LOCUS       DAOQXT010000001        92596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_211_length_92596_cov_7.684778, whole genome shotgun sequence.
ACCESSION   DAOQXT010000001 DAOQXT010000000
VERSION     DAOQXT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 92596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 92596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92596
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211_length_92596_cov_7.684778"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..1515
                     /locus_tag="PKM27_00005"
     CDS             553..1515
                     /locus_tag="PKM27_00005"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR05659.1"
                     /translation="MKKGFFIFSLSVFMVCCARKDSTVPVLGFADAFQDVTIEQAKNG
                     FLDALKEQGFDEEKGTLKLLYRNAQGNIPTLTQIINYFVASKVTLIASCPTLSTITAV
                     QNNKDIPIFMMVSPIPSLMKVNDAQDQAPRNLFGVGENLDYIDTSFLLIPTVIKPGSE
                     PLTVGMIYNQAEPQSVDALEHIKKLAADHKVNLIYQSIHSSADVQLVSASLLSKNIDV
                     FFANPDNIVFESFETIVEACNQKNIPIFTSEAGLVQRGALAAYGADLYQWGYQSGLQA
                     AQYLKTKSTEGLHWEMVKVRKNVYNPAVAAKYGIELPSNFQALQ"
     gene            1512..2387
                     /locus_tag="PKM27_00010"
     CDS             1512..2387
                     /locus_tag="PKM27_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05660.1"
                     /translation="MSQFYLTALQLALCLCPMALGIFISMKVFNIPDITTDGSYTLGA
                     VITAILLTREWPALAAMPAVMLTGAVAGVCTGLIHTKLKIDALLSGILVMTGLYSINL
                     ILLGRSNLPLINLQDLFTSRSFFTHELYNHLGISIFIVGLLVLLLHYLLKTDFGIAMR
                     ATGNNPVMTRALGINNDLVKITGLALANALTALSGYLVAQFQSFTDINMGIGIVITGL
                     GSVLIADALRVWLRVTGIGWQLLFVLSGSLIFQMVLAFTLAMGIDPNFLKLVTALFVL
                     LIVALPRVSRPLKND"
     gene            2401..3150
                     /locus_tag="PKM27_00015"
     CDS             2401..3150
                     /locus_tag="PKM27_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR05661.1"
                     /translation="MISIQNLTKIYNPGRENEVTALDEVSLEIRKNEFVVIIGSNGSG
                     KTTLLNMIQGAERPTSGSITIQGIDVTSLPDYKRSRWIARVFQNPNQGTAPDLTILDN
                     FRLAALRTQPKTLKIGTTATFKKKVRDKIATLGMGLENKTSQFMGNLSGGQRQALTLL
                     MSVMDETEILLLDEPTAALDPRSSALILNLAENLQKELGITTLLITHNLKDAHHYGNR
                     LLQFREGKIRHDVPAGEKARLSMQDIFRWFE"
     gene            complement(3678..4187)
                     /locus_tag="PKM27_00020"
     CDS             complement(3678..4187)
                     /locus_tag="PKM27_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05662.1"
                     /translation="MGIRFLGCLLWLAVPALLAAQSPCACCTPQHRQFDFWLGEWMVF
                     DTVEKKLGENSIVKLEDQCILSEHWRSTAGGTGRSFNYYDPSDSTWNQLWLDKQGTIL
                     KLKGKAESNSMILKSGWVQGQGGAKYYNRITWKKNEDESVLQLWEIMGPSDVVLSVAF
                     KGIYRRKMP"
     gene            complement(4198..4521)
                     /locus_tag="PKM27_00025"
     CDS             complement(4198..4521)
                     /locus_tag="PKM27_00025"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HNR05663.1"
                     /translation="MSKKYLVRAKYSAAGVGGVLKLGGTARKIAVEKMINDLGGKLEA
                     FYYTLNPDELYLICELQDEISGVAMSLHVDSTGMADITITPLLTPEDIDKASHIAVHY
                     RTPGS"
     gene            complement(4729..8271)
                     /locus_tag="PKM27_00030"
     CDS             complement(4729..8271)
                     /locus_tag="PKM27_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNR05664.1"
                     /translation="MPPVVHFLDTLPPPARTRLTDAPSPRVVTLNLPKKGIGIKNQSF
                     WKNTGSSYSPPEVLEALPPLVHFTNLNTKDGLALDGVTCGFLDSRGRLWFGTGGGGVS
                     CYDGMGFTNFGLSNGLASVAIMSIAEDKNGHLWFGGHEGGLSRFDGRKFTNYTTRDGL
                     SGNAIHQILLDKKGELWFITFPKGVSRYDGLNFSILDTANGLPSNYVTAMAEDREGNL
                     WFGFLNEGIYKYRNAQFIRISPESFTATTIYSIFEDSKGALWFGLLDAGLGRYHNDTF
                     TLVNLGAEKEVIPVTAIEEDVHGHLWFSTLGSGVVRYTGEKFIKYTTEEGLAGNTISD
                     MVNDQDGNLWFATMGNGLSRFNGDAIQVLSPGQGLPPQQCYSLIEDRTGKIWFVSDGG
                     GLGNYDGQSFTYYTTKQGLISNYVNGLVEDRKGNIWFGTKGEGGLNRFDGKRITTYST
                     KQGLSSEYVSILTEDRDGNIWIGTFDRGACRFNGKEMVCYSTAQGLAHNMVLSILQDS
                     RGHFWFGTAGGVSRFDGQTMTNFTTDQGLADNYINDIDEDQKGNFWFASQKGLGRYDG
                     SGIINYRAQDGLPEDLVCNILEDEQGVLWIGSYQGFSSLQYKVRSDGQATQKLIGAGQ
                     MDMGNGTLKTYDPVFDVFNTKTGFPVIDMVDGAILEKKKNLPHGVLPDKGNIWALMGG
                     GQIIQLNPKALKRKDTPREPYLKAVKIDDHAINWYDLGGQRRDSTLIAQQEVQVYGIP
                     LEGKARDSLRGIFGSIRFDSISPDYPLPQNLVLPFVHNKIHFEFGAREISRNHMIRYQ
                     YMLEGYDKDWSPITAMPSAVYGNMTEGKYAFRLKARSPEGNWSEPLIYRFRVLPPWYR
                     TWWAYALYLALAGSAIGGFVRYRYKSILKEKSRLEEQVQLRTAEVMTQKEEIASQRDH
                     LEKSLTELKNTQDQLIHSAKMASLGELTAGIAHEIQNPLNFVNNFSEVNNELIKELIQ
                     EVNGGNLAEIQAIADNLAGNSEKINYHGKRAEAIVKGMLQHSRSDTGQKEPVDINPLC
                     DEFLRLSYHGLRAKDKEFNAILETDFDPSAGKINIVAQDLGRVLLNLYNNALYAVNER
                     KKKNFKDFEPKVVVRTRRSADQLFIHIADNGSGIPPQMVDKIFQPFFTTKPPGQGTGL
                     GLSITYDVIKAHGGKIEVKSEEGRGTEFIIQLPV"
     gene            complement(8588..9073)
                     /locus_tag="PKM27_00035"
     CDS             complement(8588..9073)
                     /locus_tag="PKM27_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR05665.1"
                     /translation="MVTQDIGRVLLNLVNNAFYACAERSRSAQTPGTVNPLTQIGEPE
                     YKPLVTVGTRLVENRLEIFIKDNGTGIPESIREKIFQPFFTTKPAGEGTGLGLSLSYD
                     IVTKGHGGTIVVNKIEGPPGGTIVVNKIEGPSGGTLEVISEEGRGSEFVIRLPASQPL
                     T"
     gene            complement(9103..10719)
                     /locus_tag="PKM27_00040"
     CDS             complement(9103..10719)
                     /locus_tag="PKM27_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR05666.1"
                     /translation="MNRRDLLKNLTAAPFAAIPLSENLSTGPDEGVAGGDSIYASVGV
                     EPIINCRGTFTIIGGSMELPEVTRAMEAASGYFVQYDELAMGVGKRLAELTKSEWGIV
                     TSGCAAAMKHFTAACLTGGNPEKLLRIPDLTGFEKTEVIIPRSSRNVYDHAIRNMGVS
                     IIEVNSPEDLERAINPRTAMIYIMTGPPNDTGKELSLEVIASIAKPHSVPILADAAAE
                     NLTIPPIHLERGATAVAYSGGKAICGPQCAGILLGPKDLLMSAWQASAPHHGPGRDNK
                     VGKEEIMGMLAAVEAWTERNHEAEWNIWLERLNTIASKFKSLSGISTEIRMPTGLSNR
                     APALRISWNPNQFHITGEEVAEITARSKPRIAIGGGSGEGTSFISIVPSQMRQEQVPV
                     VADRLYAILSAERSPKSDQMASPSLQIDGPWDLEIQYFSSIAKHTLYIEQDGNWIQGT
                     HQADFSESEVLGLVEGDQIKMRSVLRRPGDSITYLFSGVVKNDEAGLPVMSGSIHLGE
                     YMTAKFKATKTKYKGARRPFMIPGGPPLSS"
     gene            complement(10766..14173)
                     /locus_tag="PKM27_00045"
     CDS             complement(10766..14173)
                     /locus_tag="PKM27_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNR05667.1"
                     /translation="MKGAGFQMVGKIQTVFLFILCCLSIPLTAQISPGHLRQYTELDG
                     LPSAEVGELLADRFGYIWAGTINGLARWDGYEFRRFLNNPNDPNSIKGLVVWSLYEDR
                     EGMIWAGTGPAYLNRFDPATRAFRQFEFSHLIDHASNVELGITSICEDDSGRLYFGVT
                     TNFQETIRGGLLFKDVRDSTIKAFPVPDSLEIQNVIHINKDQEGNIWLISYNGVFKID
                     KDRRLTRIFTREEADRSGFIPVALDPGRDGHIWITSSKGSIMDFNPKDRRYRIHNSPK
                     PQTDYYFSRISTDLKNNLWIGSNQGLIYFNKGSEQFSFFSDQAMEFPATNTVRDLEFD
                     DFGNLWIGTHRNGLYILSDKLEFKSYTPSSPGVQAFTTGWANKIIESYDGTLWITTGG
                     AIDVAGLNHLDLKSGINQKLTFYDLGIPSEAIFSIREDSPGELMVGTFNGLFNISIRS
                     KRTTPLHLKGLERMGSEAHLPTVNHFLEDEKMNIWLCTVHGLYKRAAGRDSFRRYDLS
                     AAPGGDASSNEVTGALESRKHQGLWLTTNNGLFFYDYQTDSIHRHGDGGEALDRFISQ
                     DINSIYEDSSGNTWVGTWQGGLARYDAVNKTIRNYTLDDGLPSMSIQGILGDEKNKAL
                     WLSTFEGLSRLDLEEERFYNYSLADGIQSQLFADGSCLKTSTGYMVFGGSNGITVFNP
                     SDLMQKSVPPRVYLTDLKLFNRSLLPGGDSPLKHAIYEAGEIILEYNQNNLTLEFTGL
                     HYANPSKNRYAYKMENYEDQWRDIGNQRVAFFPKLPPGKYVFRVKAANNNGVWNEEGV
                     SLKIIIRPPWWKTWTAYILYGLALLAAGYGMFRVVRMRLIEKERARSQAKELEQARQI
                     EKAYLELGKAHDALKATQAQLIHSEKMASLGELTAGIAHEIQNPLNFVNNFSEVNAEL
                     IEEMNEEILKSNYEAVQRLAKDITENEQKINYHGKRADSIVKSMLQHSRNATGQKEST
                     DLNALCDEYLRLSYHGLKAKDKTFQAEFRLDLDPDLPRISVVPQEIGRVLLNLFNNAF
                     YSVTAKCKARQGEKEFRPTVLVSTKNIKSGTGAFAEIRIKDNGQGIPREFLDKIFQPF
                     FTTKPTGEGTGLGLSLAYDMVTKGHGGTLEVVTQEGEGTEFIIKLPG"
     gene            complement(14276..15340)
                     /locus_tag="PKM27_00050"
     CDS             complement(14276..15340)
                     /locus_tag="PKM27_00050"
                     /inference="COORDINATES: protein motif:HMM:NF023431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HNR05668.1"
                     /translation="MKRFLLAGFMAIQAAFAGAQSGKQLISSTRHFVQTLSPDELKKA
                     AYSFEDTLRKKWTNLPVGLVPRPGIRYGDLSDQSRMAFHRVLSSLLSSQGYLKLTSIM
                     QLDDILNVLYQEAFDQGKINRETFTQIRNLKWEHGNYYISVWGQPQEKEAWGLSFGGH
                     HIALSCTVKGEHLSLSPLFVGTDPSEIKSSKYAGWRVLSKEEDYGFMLVNMLSESQQS
                     KAILKREVPGDIITNPNSSQRISEYYGIAAGEFNEAQRSLLEILIQEYIHNFEHAFAH
                     DLYSRILKTGLNKIYFAWVGSLEINKPHYYILHGPDFLIEYDNVGFQNDGNHIHAILR
                     ETGNDFGEDILKKHYLESKH"
     gene            complement(16667..17290)
                     /locus_tag="PKM27_00055"
     CDS             complement(16667..17290)
                     /locus_tag="PKM27_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HNR05669.1"
                     /translation="MKTKMVSAFLALSLFMLYQCAENKPAPAADAMADDAMAAKADFH
                     GYATEAEWGHHLVTVGGCNDCHTPKKMGPHGPELDSSMMLAGHPAAMPKIDVDRKAME
                     KKGYAVTMDLTEWVGPWGVSYTANLTPDSTTGTGAWTVENLFLALREGKSKGLAAGRT
                     ILPPMPWNEATRHYTDDEIRAIFAYLRTIPPIKNAVPAPLPPASAMQ"
     gene            17638..19779
                     /locus_tag="PKM27_00060"
     CDS             17638..19779
                     /locus_tag="PKM27_00060"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR05670.1"
                     /translation="MPQTRRLVAIMFTDIVGYTALMGNDEQKAFQLLKKNRDLQKPVI
                     EEFGGTWIKELGDGVMASFNTVTDAVQAAIRIQEACNRAKDFLLRIGIHHGEVVFEEQ
                     DAFGDVVNIASRLQALALPGSIWISESVHHNVVNKPGINSRYVKQEVLKNVREPVNIY
                     EILTGDQDIPEDHLVSKKTKSILFPEYDCDVYISYRAADNKQTGTTSSPGWVSELNEK
                     LRQELEATLKDKLTVCFGPSPDVENKTGETRKAINSLIFIPVISQTYCDPSSQPWNSE
                     FLKFKEEAAADKLGLNIRLPNGNSACRILPVNIHELDFEDLRLLEKELSGDLRSIDFI
                     YRDHGINRPLRPADDNLHPEAGRILYRNQINKLANAIKEIIHGIKAQAKSTDPADTGP
                     SITSRTLPLKNFITSLPSSIQVQVIDRAKPTVFLAWTSADLKEQREEMVIILQKAGFN
                     VLPTTDCPSDDELFKQKVAESLEKSSCSLHILSGEFGRRFESDEETSFPQYQFMEAKQ
                     RMEGNPDFNSFIWIHPASSNLTVKPAQQNFIKYIRNHITRNMMFSNAAGPMQLVDDMR
                     VVMMKQDSEIYDAKDTDIFFIFNQQDEAEAKVITDHISLEYPVELMNIMPDSPDQYRE
                     ISSQQIPKSKLAVVYFKYAADWALPFIKQIWKEVGGASSPTPLFLVGEDDPRTNLARN
                     FKAPKVVSSVVPKEQIPSEVKKVYTTVIELK"
     gene            19781..23833
                     /locus_tag="PKM27_00065"
     CDS             19781..23833
                     /locus_tag="PKM27_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05671.1"
                     /translation="MFEEYQICPYTGLRSFTEEESLYFKGRDQDIEQATLQLQRNKFL
                     MLTGASGDGKSSLVYAGIVPNARAGFLKSTYTQWRVAEFRPERNPFGNLCKALTRQLD
                     IPNPAITESELHHGFSALIDLYKNSDAYLDSGSIEWQQADDKEKAALKRRAGNLMIIV
                     DQFEEFFTNPENYHQGVPSKDSNLVLNLLLETARIALDENLPVYVVFTMRSDFIGQCA
                     AFRSLPEFIGFSQYFVPRLNRLQLQQVIEEPAELSGNRISRRLTERLIHDIAEGVDQL
                     PILQHALNQIWHAANKGKEEMDLIHYAMVGGMPASDLPDDQTGMFKSWFDQLPEHIRA
                     CYHRPSLQNVLDTHANKLYESAEEYYTAKTGKPLPDETSKAIIRTAFSCLTKIDQSRA
                     VRNRMTLNEIWQILNRKEVELETVGQVLNIFREPGNTFIRPFMTEDPETANLGGENVL
                     DITHESLIRNWDSLKTWAKAEYDSYQVSQDFEQQLGRWVQSGKSNNFLLSIGPLTYFE
                     NWFKQVKPNSWWIARYLPEEEDAGKKKTKAEALIANANEFLARSAQKHVVTRTVMKLG
                     PRKIAAALGLLVILTLSSFALSNYLSKRNSAVLKTIKKESLSLANNPKLGLEFYVPNL
                     TQLMQEGSLTLPEIMESIKDPWQKIHVSTGIAKLLQMQGKNEPAAEMTRAFSISDSLL
                     TLFDTTADDQVPLNSLLNEQYNLKIALGFALLHSTDSTWAGLARKNAVRSGLWAKRIL
                     ADPPGDFNEMRLLMLGLEDALHYRVLIPEDIHALIGQLSPFSPNQASERLSRLFDRDQ
                     LQVRGTQDYGYMFNALFQDLAYLYAAAGDEEKALWCIDSLLSYPQGYLENNYETMADN
                     ATNIAAHFFTYGHLDALERFIRGYSLKKGIPSHEFYNRMISRSLLDHTSTNTYNYYNV
                     VGQAYTNMNLKFSGDDFMYFAFRKYREAIQQIADPDQRSLNLAVAFKNEAILLSFRNA
                     LRGSPFPQVRLDSLFDLSLNHYRQVRAEYLSQVNPVVSSSEVDRINVPNSEQYLFPDY
                     FVPFHPFEPRGYLFQFNDPAFVDYILRQDLFKELYRTDQDIRPVQRWLNFYNAVSISR
                     DYFLKEAIPADLMTGLAEKLEAGGFEQRNDLNLLYLHLGNTLSAQNKKEEAWRYYQKI
                     QPQWVLNAFQYKGMNFLNTYSLEQYGIAIGHLFSSQHDQKALDLIGAFKKPVNRSSLY
                     AFAAQQIILQDGQAEIYRRLIDSARSEMNRIDNPAAFQPNRHNLAIALMLDDPERNAT
                     DAYQIIKNSPGKLDALGRFGTALSRKGLLYKAWSEIPTDFSDEDRSWFLYKILYGYNH
                     RIPTKPEWKCFEDNMVFVDRYFLPYINEND"
     gene            23826..27884
                     /locus_tag="PKM27_00070"
     CDS             23826..27884
                     /locus_tag="PKM27_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05672.1"
                     /translation="MTDPGAFMQHQTICPYPGLRSFTEEESLYFKGRDQQIDQISSLL
                     EQKKFLMVTGASGEGKSSLIYAGLVPNARAGFFKAKYSNWIVADFRPERNPVTNMARA
                     LADKFDLAPASVETELRRGYSSLIDLYTNSSYYADEGDEAWKSLSAEEQKQKKRQSAN
                     LLILLDQFEEFFTNPENFANEAPSQDAQVVVNLILETARLALKNDLPVYIVCTMRSDY
                     IGQCSAFRGLPEYIGFSQFFVPRLKRRDLKLVIEEPAMLSGNRITQRLVERLVYDLAE
                     GIDQLPILQHALSQVWLAADHGREEMDLLHYALVGGMPSSDLPDEDQERFNTWMKGLP
                     EYKRAYFAETGLQRIIEIHANTLYEGAWEYYSRQNPDKPISRQDSKRIIALAFACLTK
                     IDHSRAVRNRMSLAEITSIINLPHLDSRTVGQVLNIYREEENAFIRPYKSGEPETQQL
                     EPNSVLDITHESLIRNWNKLIQWANKEFDYYSTWQDFKKQMARWTESGKEPGYLLPIG
                     PLTYFENWYNNCKPNISWIRRYEEAGDPALTEQQSVQLLGNTREFLKKSASKVRFTRA
                     FIKYGPKRIATVLAILFMTVLSGFYWYDASQKQNSRVIDRLQQQASDLVRSEEVAQIV
                     KALYLITNERYRAGSLMEHLRSLQPKDALNNGIEVYKQLIKLDMKRDFGLKTGLTGFI
                     DSTYRQLASTEPDAFYLITKLNEYTLLLTYDQYYRPGPVLGERLKQASFYGYKLVEKF
                     LDHPEMASPTAGVELNCSLQNWLTFGQASAVEIQSLLKRFSPFEQDSVNPVFNAYYRK
                     GTYETNGRIPNDYNGGYHTLASLYAALGRSDQVIRCFDFLRGTGQNDYFTGSLFNNYT
                     HILGIFHQFGYREQSGDMIRWLGRYYPTNTPLTIHRNAVIRAGYLSHLYRVNVDRAVL
                     QSYRGYFFPNLCLAPPGVFESLNRDYEALIQQVPDPNERNYLFALNFKRIAMFGSKLA
                     FDRGLPADTARLHGWLAKSVAHFRAIPKEYLQQTVSSTLPYFADGVRTRQVSRKDLLI
                     YPDYRDGWFSWTYHTDLFFNYLSRENLLMEYYPTAGDLEALNFWLAKAYEIKPGNFRE
                     AYDHNYPLPDEVLTTLDQFIDRHPEGGRFDRNLLTLILANRAFEKKDSSQAMKYFLRI
                     DQSALTRSSNRYEYLEKTFFSNQVKDLAVHLADAGRLMEAARLAEWFVSDNDKALVYM
                     HLTHQLYNKGDPYSFTYLDSVFSKMEKVDFTLFNGPVLDVRYRLTRLLGQIGSDRLNQ
                     RNREMMRQLAEGPKTLALNHMMLGVSQEGNYYRAITSIPDRLTEAEELACLTLILNQA
                     AAKQESPDQRQAWASLDHWFNYFYYYIFFVPN"
     gene            27884..28684
                     /locus_tag="PKM27_00075"
     CDS             27884..28684
                     /locus_tag="PKM27_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05673.1"
                     /translation="MSRYLSYYYLLFFALITGAFASMAQNAYGMTILGIAAICFGLLF
                     ALQWLHTWNQKDRNGLALTELTCLVLLSVFLALRVFNIHFQSVEILFTGTALLLAMVY
                     GIRLFRIIQPDKRLAGRMTWLVLCYYLSLIFFLLAVSLAPLIPGAVSPSGILAFILLF
                     LFVTGSWISRNQIQEGEKISGFQVVGRFKDRSALLLSLFVLFALYTGLTRIGWLPKMY
                     TSVYPQTYYQLLNEAESGKDIPVNGKYRHEAFKEEYDRFSEKHLKNKE"
     gene            28768..29736
                     /locus_tag="PKM27_00080"
     CDS             28768..29736
                     /locus_tag="PKM27_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNR05674.1"
                     /translation="MILTLLLLIIGFVLLIKGAQYLVGGASSIARQKGISNLVIGLTV
                     VAFGTSMPEMTVSLVAALEGRNDASFGNVIGSNNFNLFFILGLTGLIYPLAVHRNTVS
                     KEIPMSLGLALLLIVLVNDTSLGLSRTGFDILSRFDALILLLAFGGFLYYVYINTVKS
                     PETEVTVEVRPYSVPKSVGFIVLGLAGLIGGGKLVVDSAVTIAQSLGLSERIIGLTIL
                     AIGTSLPELVTSAVAAWHKQDDIAIGNVVGSNIFNITLILGLTGMVHPIPFNPAINRD
                     ILLLICGTVALIFFLFIFTRHKLERWKAFLMLATYIGYTVYLVQEG"
     gene            complement(29813..32401)
                     /locus_tag="PKM27_00085"
     CDS             complement(29813..32401)
                     /locus_tag="PKM27_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR05675.1"
                     /translation="MPQTHELAAILFADIMGFTAIMQEDERMAMKYREKLKKCLETEV
                     KLYGGRIVKYTGDGALCSFKSAIDSIRAALVVQYNMLESPAVPLRIGIHQADVIVEED
                     DVFGDGVNIASRLESLALPGSIFISAKVYDDIKNQTDIQTVSLGKYILKNVKEPLEIY
                     AVSNPGLKVPLKKRLEGKGIKYTRFKSLKRKKYFLLQWLLPLLLFGGIGAVVLPAWKK
                     KQTARKDWIPQIQKMVEENSIIPTRAFDLAKAASRYIPKDSSLILLWPQIAASVSFET
                     RPSGAEVFWKDYDDLKGEWKSLGTTPLTNIWFPRGFTRVKIEKPGFITVYNPRIRNLT
                     LDSLGTYPEQMVRVPASEAGMNIVGLEQHGGKWVGEYLIDQFEVSNKAFKQFVDAGGY
                     RDSVYWPQVHSAGIPLPFEKAVTLFVDKTGRPGPSSWEAGTYPDGKDDHPVSGVSWYE
                     AMAYARFAGKTLPTVFHWSMVANTFNTWGIIPKSNFNGQGTVTAGSLEGISYWGVYDI
                     AGNVREWCSNASNKKGYRFILGGGWNDPTYAYNDGYIQPEWDRSPGNGFRCMKTFPSD
                     TTLGTLAGQVDFAFRDYSTEKPVDEKTFQVLLRQYAYDKTPLNPEVSPIEDSGAWKIE
                     KIELDAAYQHERLTAYLFLPRNHTPPYQTVVFFPGSGVIFERKFSTSSGNSSSRAFDF
                     ILKSGRAVLYPVFKGTFERGDQLSSDLQAETKFYRDHVISWFQDIGRSLDYLETREDL
                     DIQKIGYYGYSWGSAMGGIMCAAETRFKAAVFHVGGLMMQKTFPEVDPLHFFPRVKIP
                     VLMLNGKNDTFFPVETSQKPMFNLIGSPANDKKIIIYEGGHLVPRQELVKETLGWFDK
                     YLGVVN"
     gene            complement(32431..34110)
                     /locus_tag="PKM27_00090"
     CDS             complement(32431..34110)
                     /locus_tag="PKM27_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR05676.1"
                     /translation="MSPLTLKSLLNKRSDFTAWLQSWQEMQGNALAVEDQHHQLIFGE
                     AVAGQSESFPVNLNDEILGWVKGGPQARLIAQLMSLQLQKEAEKKKLGAEVLSLYQEL
                     NVIYNFSEKLSQTIEPEVIAKLTLEQAIHSIPSHSGVVVLWNEETKQLHIPARSGLPL
                     FNEEKLRSQPGLLLKIGLGGQSEIVNEFDVLKDSGILEAPVQSLIYAAMKAKHRIMGA
                     IILAGMEAEQYSAAHLKLLVTLALQSSTAIESAMLYEKNIREVREREEAILRIHEVTK
                     KFVPSEFIRALGKDSLTDVMLGDQVGKNVTVLFTDIRDFTTLSEKMTPEENFRFVSAF
                     NARLGPVIRAHRGFINQYLGDSIMALFPDAPEDALEASIEMQEAIQDFNLERSREGLQ
                     AIKAGIGLHTGPLIMGITGDEYRLDAATISDTVNTAARIESLTKYYRSPLLLSGDALL
                     QIQQAERYHIRHLGKVQLKGKYNVLNIVECINGYPAEELQLKLRTLDYFNEAMSAYLN
                     RDFGLAAEGFQQVLRLDPEDHAAKYFINIANKYQHQGVPDNWSGAEAMMHK"
     gene            complement(34169..34549)
                     /locus_tag="PKM27_00095"
     CDS             complement(34169..34549)
                     /locus_tag="PKM27_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR05677.1"
                     /translation="MAQKILIVDDEAHIRMLIEQTLEELEDEGVEFITADNGEKALHL
                     IQSEKPNLVFLDVMMPKMNGMEVCHKVKRELGLEGVFIILLTAKGQEFDRQKGQEVGA
                     DVYMTKPFDPEMILEKARQVLALS"
     gene            complement(34629..41153)
                     /locus_tag="PKM27_00100"
     CDS             complement(34629..41153)
                     /locus_tag="PKM27_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNR05678.1"
                     /translation="MKLKRPCSTRFLFLLFPVAFCWTGIFSRVFSQEHSVKGLPFITN
                     YRYQDYNADGVNWWAEEDDQGVMYFANALGILIYDGQHWDLVKPPGAPETRSLVKSKD
                     GTIYVGTNGDLGYLARGKKGQLEFISLKEKLPGPHRQFAEVWETQIFRDEVYFRTNNK
                     IFVWNGSAFRVLESKEGFHVGAEVKGRYFVRIWTRGLTELRQDSFHLVPNGEKFANER
                     IYVMLPYDDQRLLIGSRTQGMFIYDGKDFTPFKTEADPFIVNTSLYGGRILSNGLIAL
                     NTFNDGMVIIDKQGKLIQRIDKSVGLQDNSVDQVFEDSRGILWMPLFNGIAKFDLRSS
                     LTFYNEALGLPAKTVFSVGTHQDQIYAGTNNGIYKLNPVTNRFEKVEGSSGQTGNFLV
                     NGKDLLVSGAERGLIKLEGNKGIAVIPGINYDFHLRSILRSKLDTTVLICALRSGLAI
                     VRYSPLTGGYAVESFAPILNLGIGLISETPDGNLWLSGEGIGEVKLVKPDRSSGKWLL
                     NENAVEVFDSRQGLPRKNITILEYEGSVYFAAEADSVFVFDEGLKRFIRDTGVILNKQ
                     FVYNADYTLLGITDPRGRIWANFGRGVFVAIPAAGGKHKIVNAPFKELKRNYPIWDIA
                     LSAGSKGEVVAWMSGREGIIRYDGNLDESVQDPFSCILRGITLNEDSVYYAGFGPPSG
                     KALFDKGWNTIRFDYAAPFFKLENETRFSSILEGRDKHWSDWDLQNSREFGNLAAGDY
                     TFRVKAKNIYDTESSEATFPFTVLPPWYSTWWAYLAYLLLAGALVYAIVRWRTRQLQE
                     KHRELELMVNERTAELKQRVDELAVINSVQDGLARELDIQGIYDLVGDRLCTLFPDSQ
                     TLVIRTFDHEKGLEHWHYAKEKGVRQIVDPRPLNWNSRQLIQTKKPLDIQEHYVETAK
                     KYGGTGVTAGQAPKSAVFVPMMLGEVVKGSISLQNVDREHAFNESDLRLLTTLTNSMS
                     VALENARLFDETTRLLKETEQRTAELAVINSVQDGLARELDIHGIYNLVGDRLRQLFP
                     DTQTLVIRTFDHETGLEHFQYAVEKGQHIEVAARPFNWANKLLIQTKKPVDIKENYVE
                     TAHKYGGTGVTAGQAPKSAVFVPMIVGEVVKGSISLQNVDREHAFSESDLRLLTTLTN
                     SMSVALENARLFDETNRLLKETEQRTAELAVINSVQEGLAKELDMKGIYDLVGDRLCE
                     LFPDTQTLVIRTFDHDKGIEHFQYAIEKGLRLTVESQPYIWASKHLIHDRKPILINEN
                     YLETSKKYGGTGVTKGSPPKSAVFVPMLVGDLVKGSISLQNVDREHAFSESDLRLLTT
                     ITNSMSVALENARLFDETTHLLKETEQRTAELAVINSVQDGLVREMDINAIYELVGKR
                     LCDLFNTQTVLIRTFDHAAGMEHWRYVIEKGARHYSEPRPLIWANRYMIEHRKPILIN
                     ENYLETAKKFGGSGVSMGKPPKSAVFVPMIVGEVVKGSISLQNVEIENAFSESDLRLL
                     TTITNSMSVALENARLFDETTLLLAESKQRAAELTTVNNISRALASQLDVHDLVQMVG
                     DQLRDLFKANIVYLALWDPKSNMIDFPYQYGEDMPSRKLGEGLTSKIIRDGQPLLINK
                     DVDETTAQLGVQPVGVSSASYLGVPIPVGERIIGVLSVQSTEQENRFNENDLRLLTTI
                     AASVGVALNNASLFEEVKEAQAEAEAASKLAEKANEAKSAFLSTVSHELRTPLTSVLG
                     FAKIIKKRLEERIFPATDISEPRTEKAIQQVSENLNVVVSEGQRLTSLINDVLDLAKI
                     EAGKMEWNQESVFLPEVAERAIAATTSLFDQKSIRLIKEISTDLPEITGDRDKLIQVM
                     VNLISNAVKFTPQGTVTCKVFRDRDEIVVSVSDTGIGIAEKDHAAVFEQFKQVGDTLT
                     DKPKGTGLGLPICKEIVEHHGGRIWLESELGKGSTFLFSLPVTKTKTPRPQHLDELVK
                     QLKEQVALSPLSVDKPAAHILIVDDDDSIRSLLQQELGDAGYQIEEARNGKEALAKVR
                     EGRPDLILLDIMMPEMNGFDVAAVLKNDPQTMDIPIIVLSVVQDKARGFRIGVDRYLN
                     KPIDTGILFNEIGNLLEQGKSKKKVMIVDEDSGTIGTLTEVLKTKGYTVLESNVLELV
                     EKALVHQPDIIIINSLLSGNQESIQALQFEKGMENVLFLVYH"
     gene            41293..41889
                     /locus_tag="PKM27_00105"
     CDS             41293..41889
                     /locus_tag="PKM27_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNR05679.1"
                     /translation="MDPGFMGNAGMEIQSKSDEKPIRYFVALVCPSPLQDRINRLKSR
                     VKEEYHCRVPMNSPPHLTLIPPFWIEPRNEPLVFHALDVFQYPSGIPQIRLQNFSHFD
                     GRVLYIRVVEDPSLLELKTKLESHFISILGGAVVPDVRVYHPHITLAQAGRRRSAFHR
                     ALEYFATGEFEDQFHADKISLLRHDGVAWQVIAEKKIE"
     gene            complement(41892..43964)
                     /locus_tag="PKM27_00110"
     CDS             complement(41892..43964)
                     /locus_tag="PKM27_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR05680.1"
                     /translation="MSQTHRLAAIMFTDIVGYTALMGQDERKAFEFLKKNREIHQEII
                     GEYQGRWIKELGDGIMASFESARNATLAAIKIQEACRAAGLYQLRIGIHLADVVFENE
                     DVFGDGVNLAARIQAMAPVGGIWVSESVHQNLVNKRDIRSEFVKTVELKNVREPVKVY
                     RILLEGDPIKSPVVRNSPQGSKQKKYPWSGRKVLIYLLPALAAGFALIYFNLKNTRDQ
                     SAHSSDPGAAVDPSIAVLPFTDMSPDQDQAYLGDGLAEEIITVLSGINNLRVIGRTSS
                     FQFKGEQMDLREIGKRLNAGTILEGSVQKSGDRIRITAQLIRTSDHSHLWSQRFDREM
                     TDIFKIQDEIAAHIAGRLKLTLNLPGNNLPANQVTSPEAYTLYLKGLHAYNAQQYEQS
                     IAYNQEALQLDSNYAASYAYIALAKAWIINKQRDFQNKAALADAQNYAYKSIALFPGL
                     AEGYSALALLAWSIERDFPAAREFFEKSLQLNPNDALILNRYGYFLLWMADFDKASAL
                     ASKAMELDPVDYNSYFILIRVNQATGRLDKAAQLIREGNGLFPDRQNLAFFEVNNADL
                     SGDYTRVILLCDSILIHKKSLTPEFLAKLCIAQFKTRRFRESEAILAQLKTLAAEGAE
                     DAGFSLATVYASRNVPDSCFYWLNRSLERLETPLRTLKIDRVFDPFRKHPQFENIYRQ
                     YGFDRYPQ"
     gene            complement(44327..44764)
                     /locus_tag="PKM27_00115"
     CDS             complement(44327..44764)
                     /locus_tag="PKM27_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNR05681.1"
                     /translation="MKISCLKFMFCLLIFSARMNAQQTFQSMENNKHKIVIQFNEADS
                     ISQQRVTLQAAHIKDDLPGAEVEVVCLSGGIDLLITAYSKAKDAVAELTKKGVVFAAC
                     NHSMNSRMVTKTDLLKEAVVVPSAVIELALKQEQGWVYFKGGR"
     gene            45084..46514
                     /locus_tag="PKM27_00120"
     CDS             45084..46514
                     /locus_tag="PKM27_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNR05682.1"
                     /translation="MDELKMINNGSGASVSGADLETFKSNFRGRLVIPGDADYDEVRT
                     LWNGMHDKKPGIIARCTGVADVMASVNFARDHQILLAVRGGGHNVGGSASCDGGIMID
                     LSLMKGVWVDPNTRTARAQGGATWGDVDRETQVFGLATPGGVVSTTGIAGLTLGGGLG
                     WLRRKHGLTIDNLISVDIVTADGQFHTANSMHNADLFWAVRGGGGNFGIVTSFEYQLH
                     PVGPMVTLCAPWYPAEEADAILPVWNEFMKTAPEELSSTAMFWTVPAAPGFPAEAHGK
                     RALIIAAVHSGSLEDGARIIQPLRELGKPMLDLSGPIPWTALQSAFDGFFPKKQQLYY
                     FKSRNLRQLDQQTIQAIIPQACKPPAPMILIAIWNYGGAMARVGEQETAFTGRQVPIL
                     FGVDAVWADPGQTEEVLAYSRNFLSSLEPYSTGGMYVNFSGLGEEGETLVKSAYGNNY
                     ERLVSVKNKYDPHNLFRLNQNIKPTV"
     gene            complement(46528..48459)
                     /locus_tag="PKM27_00125"
     CDS             complement(46528..48459)
                     /locus_tag="PKM27_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNR05683.1"
                     /translation="MSQTRQLAAIMFTDMVGYTALMGRDEKKAFSLLQKSRDLQKPVI
                     EEYRGRWIKELGDGVLASFTNGADAVQASIVIQNKCAAAGEFELRIGIHQGDVVFESG
                     DVFGDAVNIASRIQAIAVPGGVYISEAVRFNLTSQYHITTRFVKTEILKNVKEPLNIY
                     EVVLNGAESKSDPSGAKDRTQTIEKSIAVLPFVNMSNDPEQEYFGDGIAEELINSLVH
                     IKSLKIAGRTSSAQFKGKQVDLKEIGRRLGVAHVLEGSIRKQGNRVRITAQLISVQDG
                     FHLWSEKFDRDIQDIFAIQDEIALAITEQLRITLLDKERENLVNNATQNTEAYELVLK
                     GRFYMNRRGSAIPVAVKYFEQAIAADPNYALAHSGYSWTNYLVAFYGYAPGTAVLRKM
                     EESAEKAIKLDGELAEGFFALANYYAAFVWDWDKSQKAYLKAMQLNPKFAQAFSIYGM
                     GYLCWIKGQFEEAENLSRAGIKADPLSAVDHADFAWTLYGCGKFEEGLAMARMGVELD
                     GNSFLSHRLVGLCLIELNRTEEAIAVFEHLLSISQRHQHALCGLIWAHCRNGNLIQAQ
                     AVFEELLLSKKHGYVVPFYTGVSAAHLNRLDLAFEYLEQAFADRDPALIQIKYNAFVP
                     ASLKEDPRFSDLLKRIGFP"
     gene            complement(48551..49144)
                     /locus_tag="PKM27_00130"
     CDS             complement(48551..49144)
                     /locus_tag="PKM27_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05684.1"
                     /translation="MTQKIHLLLLLACCIWVGAKAPAQILLSKTAISNGGQPVAGNTY
                     KTQVTVGQSLAGISRTIQSEAAVGFWYLPRFSSSRFKFAKNPTAIPGRIPTGENGGIY
                     ELQRHTHAEKINALKIYPNPVVSQASVEFQLAGDGNVHLALYDFKGNRMFTLLRGKLE
                     AGRYKIEFQSDQLPAAVYSVVLTSEQIRLQEKCMVMN"
     gene            complement(49149..54602)
                     /locus_tag="PKM27_00135"
     CDS             complement(49149..54602)
                     /locus_tag="PKM27_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05685.1"
                     /translation="MKTFMFPLLALLYLYPFWLRAQSPRTINIQGVLSQASGQPVLDG
                     QYLLTFRIYASAQESGFLWEEKQENVQVQAGVFSTILGKINPLTLPFDKTYFLGIRTG
                     QEPEMTPRIELTSTPYSLMAKNVENNAISSEKIQPNAVTIDKISSPLISSLNGVTNDG
                     GNINIVAGANITILSNDQDNTITISAVNPGTGTAGISRINGGNAISVTGPDGPTTTIS
                     VNPNAITNNEIQNNTITADKIVPNVLSSLSGVSNDGGNINLVAGNNISIVPNDATKTI
                     TISAAGSGGGTGTINQLSEGTGISIQNPFGPNTTIGLKPNILLGPAGSLHLLNQNSLA
                     VAGFSTSASNLGGTLSLSNGQGTQTFGVTHFPDGQPLLSLNSRLGRQVVDIKANEFSD
                     GELNLKSVLTNTTVRLGSNDNAGGLVHVFNKAGILASQLGVNARGDGRLKLNSVNNRT
                     ALELESIAEGAGILRLYNEEETPVLRLTTSESLGGRLVIYNRSGNEAVSSSSNNSGDG
                     GLFIRSGRDRLMIGMQSNTDNGGGMLGVFNEEGNETIRLSNFMDNQPFIILKNRLGNV
                     VSELKVNEFSDGELTLRSVLNNPTLSLGATDNAGGIINVFNREGQTMAAILENGNGGF
                     IDILNNDGTEVITMGITTDDLNARINVNNRLGNSSAELFANEFSDGQLTLNSVLKNPT
                     VIAGANDNAGGLIQLFSQDGKRAVRLTEVTGGFLGINNTDETEMIQIGTLEDRNGLIR
                     VNNRLGNPVADMQSNEFSDGQITLGSVEKNRTLLLAANDNAGGLINVFNRSGSVSAQL
                     TETEGDGLFKIFNRDSQLVHQLGINAYRAGLFEIKSIFNNPENVAEMGGNEDDEGHLY
                     LFNREAKTAVYLSAAGGDGDLGLNDISGKVKASLSGSRYGGSLSLRNEANQPVHYMGT
                     FGKSGEGFYSIQDKSDVNKNRLHMGGDTLGNGFLNIYNYNGLNTFFMGHNAFADGTLS
                     LKNNMGNLGAVFEALRDGGSVTVTDGKSGTNHRGVLLNGLNGGKLSLFNNQTKLVNVI
                     GVGENGEGLWQIVSKQDLTKNRVEIGGDNSGSGFAKFFNGMASLAADLGSFPGGSGFV
                     STYGPSGKRQTHLAQFGNGGAVYVMDDNENAVGTLAYNGTAGLAWVTSKERKGGAFMT
                     HNSRGGDIHVRNNAEKIGAEMITAANLGGEFNIYNANGGNTARLSNGSSGGGLIEVGT
                     AAGDKVGRMTTLEGTGYLGLDNIDKLEVVRITANQGKGGGMALRNDKGFDMIRLTQDQ
                     SHGAIVVNNATGGLMSILTRTTLGGGFFGTKDHKGGDAVWLTTNDQGGGNLTTFNDQG
                     KVAATINVNTAGQGKASVNGPGGNELATLTGTVQGTGLVNIFNNAGKVLAGMTNNTVS
                     GGGYLFVNNSAGTFLSGITTNNSSGGGYLHANTSGGKDVARMTSSNGGGVVVTDNPNG
                     AAVSYLSFNNNNGGYIGVANAAGADKARLTINNGGSGIVSVSNASGRDVVEASVTTDN
                     HGTVATYNSSGAVIAGLLATSSGHGYVRASNSAGSERASMFSGNLGGSVNVYDVSGMQ
                     RVSMSGGGNMTVTDADGSYLSNYSTGSGVNLILVDRFQHPRAEIKSGILLAKGPNGIE
                     HSFMSTTNGSNLGYIGVCNSNIVGSPVKAGMYTDNSNQGVLFADIKNFKMDYPGKPDK
                     QIWYGSLEGPELAAYIRGTGKLVNGKASIEFSDHYQKVANTGTMTVILTPLSGKSKGL
                     AVVSKKSTGFEVEELMEGSGTYEFDWEVKCVRNGHEDFKVVRNKTDDPKPFNPGVEYP
                     AKLESGYPDKMDANPILHGASIIKDIEKAQKSQ"
     gene            54766..56235
                     /locus_tag="PKM27_00140"
     CDS             54766..56235
                     /locus_tag="PKM27_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNR05686.1"
                     /translation="MVFPSLNSVRFRLERLERMMDRIAIQKSALVQTVHPAQRMGATN
                     LLQYLSLRNVDIRDLQDDLHILGLSSLASSESHIQRQLQAILQRLGKEYKRGSLNPCD
                     YRLSRKEIFRQSKLLFGIKRDENIPHIMVTFDSSFADDYFFIKQLLLDGMNVARINCA
                     HDDETYWSKMIRNLRKACRNTGKPCKIYMDLPGPKIRTQLLKKGSADGKVTVKEGQLV
                     WLADDSRGFNKNDIVIGTGEPGIIPGLKTGDHVHIDDGLIRGKIVKKSKAYAQVRISR
                     ISSSKKRIKAEKGLNFPDSSLRLPPLTEYDKSCLPFISKHADLVGYSFVRHPKDLALL
                     QDLLKTYKKQTPGIVLKIETPEAVKNLPALLFQGMREKSFGVMIARGDLAVEIGFERM
                     SEIQEEILWICEAGHVPVVWATQVLENLNKTGLATRSEITDAARAAMAECVMINKGNH
                     TLEVIKTLQDIFTRIGGHHIKKRYTFRPLSIATHFIRNR"
     gene            56564..62950
                     /locus_tag="PKM27_00145"
     CDS             56564..62950
                     /locus_tag="PKM27_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HNR05687.1"
                     /translation="MRTSKPFNLTVFLILIKLSFPALQGQTLQISDFALYGKNKVLIS
                     SSNSISGNGSIGSLSLIQSTGNIVLGGGVHSGGTIALSNGNTVYGEITAANLANLVTP
                     VLTAGSSTSFKGNLRVNGNMVIAGGMVDGNITQPTGASYSGPAPAGGRSLLPSPIPLP
                     SLPTLPVIPVFVPGNTDITNNAVLSPENGMGKWRNLSLSGNRTITFDGPGIYVFSSIN
                     LSKNNTFKFDFKGLPGTFQILVAGEMNLEKIAVEYAGVPNNDASAAASRISSFTNGTG
                     TAFSIANGNGGSKTRWLGTVYAARGNILIGSGTGSTEVMGALWSGAEVVLNSGANFSF
                     VKPADCIPPGANAGPDQVLSCTVSSVSLQGASSSPFAQFHWQAESGGLIASGASTPTP
                     VITKSGKFILTVTDPFTGCASKDTATVVYDPCIDPGYESGTTKVNDLIGSELTALFEQ
                     GDFNPEHQLFLLDSNQEKVFIEIIPLAGQYSQVLSLLQTAEYGLDQVIPNPPGILVIT
                     GRFPIMNLNKLNDLPHLIQYVRPLVSPVAGNGIANTAGDKAMGADFARNGFGLDGSGV
                     KVCVLSDSYNTLDNRAALDVQNGDLPGPANPEGNLNPVQVLLDYPFGRKLDEGRAMLQ
                     IIHDLAPKASLAFRTGFLSAGDFANGVIQLKNSGCTIITDDLTYVTEPFFTKGAVAQA
                     VDNVNSQGVSYFTSAGNFGRKSYQAIFTPGPIPEGNNLSGNAHDFGGGQVLQKIKLNK
                     AGAYTFVLQWQDEVYSLGQSVTGTQNDLDIYITDEEGRVLYGFNRNNFGGDPFEILPF
                     QVTAATEANLMIVRAQGSGNVNFKYIIFRGDAEILNYSGSSTIFGHANTQGAMTVGAV
                     LYSNTPAFGVDPPTIASFSSVGGGLINGLIPNKPDFSAPNGVNTTVSFGSQDIDGDGL
                     PNFFGTSAAAPHAAAVAALAYQARVKFGYGLPTPSDIRQLFINTARDMHAPGFDYSSG
                     YGFIQADAAISSLPSSPKPVITSLEFPEGTDLLNGSELPILLTVNGEYLTEQSVIYFR
                     DQELTTIYIDGNQLTAFIPPFSGNPAITVYTPPLSPSGQDGGYSNSITFLDIPKKNVQ
                     VIAENKSKKFGERLPEFTAGIWIDGIPLSESGLTLYQLGLDQLRFSTSATVMSPVNQY
                     LLRPYRTFDPEDSRDAGLLDLYNYEFVNGILSIRSMPLKIVPKNQTVVYGDLLPGVEY
                     DFIFDDSNMDHGDKAAFLEGIRNEYLSGFKSNHWVLFNSYPESNIVLQASDLENMGLL
                     ASNRSVSNAIPFINGVPFLNGLPEGNTSPVTSFINLNPQSILDYQINSSEAILRNGTP
                     FINGSPLFNGIPYLNYAGIFNSIPFLNGEATVRYENTNSVPFINSVPFINGTPYVNGT
                     PYINRSGYEEINGTPYVNGVPYLNSGFELINGTPYLNGTPFLNAGFELVNGIPYVNGV
                     PLINSGEIYFINSVPFVNNNGLYLINGVPYLNSGFEIINGTPYVNGTPYLNSAFSLIN
                     GTPFLNSVPFINSLLEIINGTPYVNGAPFINNSNGFTLLNGNPYINGVAFINSGIQVL
                     NGVPYLNGVPFLNGSLELVNGVPYLNGYPFINSSSDILINGTPFLNQHSELASIIDED
                     DLSLGDQSLILHSVNGLTGLTAGDHFIIPGGYTPGGLYNGNLDITYGLGNLSISKAVL
                     TVTARDTFMTYGDPVPLFTGSLSGLRYTDQVQVNYIMVDGNGQPLINPGAGSHFIQPQ
                     VILSEPVNYQVNLVQGQFTVAKRNLTITAVDTTREYGLQNPVFTLAYSGFASGDGISA
                     ITTLPAISTTASILSDVGPYPVTLSGGFSPNYNLVYSPGILTITKAPLTVTANDAGIS
                     EGEPLPAFTATYQGLRNQDALLIGPGSLQFAVFDSNQNPYSGAAGTYIITPYNLNFSK
                     GTNYQITYFNGFLYVNPTKNVRHIRPFLDCVEDMGAQATGLRYKARFYYINDNDITFY
                     IPEGEDNKITSAGVYSGHPPVLLLPGTHYFEIYFDGKKMTWSVRTKNSDKKSASSSEA
                     SSTSNKCASSSNLAGRNPSPGLVQGSPAGAWGLIAIFPNPVQSDFSIISSREVIHSTG
                     LEMVDLAGRRVNAWQANQLDEHSILIKAGPLKTGIYFLRIKLQDRIETIKWIKL"
     gene            63044..65002
                     /locus_tag="PKM27_00150"
     CDS             63044..65002
                     /locus_tag="PKM27_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR05688.1"
                     /translation="MFKNCFVFLLFLSILPRTSGQMELVDSLKLRIETAEEDSAKMKL
                     YLDLSQAFYNNSWDSAIHYAQLARTIATKLEHHRGTALAYKNIGLANYFKGEPLLTIE
                     AWENSLLFFEKAMDKQGIANILSNLGGVYERSDEARALDYYLRSLILAEEIGDHFRIA
                     TLSNNLGALYDKKPATVDKALENYQHAIAVSEQYMDTDPRFAEAMGIATGNMGEIYLK
                     RDQVDSALFYFRIGEKYLQNKSSLPLALNNIGKAFLKKLDFATALKYQQQAYEEAARP
                     NNKMYMTFALLGMADTYAQQGLTAKAIELYKKAESYALELNINDELRLAYAGLASQYS
                     KTGDFAKAYQFQLKLTSINEVIYNNETDNKLANLRLDFDLKKKENEISLLSSEKSLQD
                     LALNRQKLIKNALLAGLALVFLIIFILYRNYRQKTAVNKLLDKQKAEIENLVLNILPE
                     EVALELRQNGSATPRYYESVSVMFTDFKGFTNLTDQMSPQEIVSELNTCFMAFDNLIE
                     KYHLEKIKTIGDSYMCAGGIPTPQKDHCIRIMQAALEIQQYIQNFNLHRGKSGLPPWE
                     IRIGIHIGPVVAGVVGKKKYAYDIWGSTVNIASRMESSDEPGKICISSNAYNLVKDKF
                     ECTYRGKINAKNIGDIDMYYLVKEIHHA"
     gene            65965..66933
                     /locus_tag="PKM27_00155"
     CDS             65965..66933
                     /locus_tag="PKM27_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNR05689.1"
                     /translation="MFLSVILLLLGFGILVKGADFLVKGASSVAKRKGISNLAIGLTV
                     VAFGTSMPELTVSVLAALEGRNDASFGNVIGSNNFNLLFILGVAGLLYPLAVHRNTVN
                     KEIPISLGAAFLLWFLVNDSWNGMGGSGINGLSRLDALILLIFFSAFIYYVYLSMKEA
                     SAKNAEEGYHQYSMPFSIIMILGGLAALIGGGKLVVDHAVNIAQYFGWSEKLIGLTIL
                     AIGTSLPELATSAVAAYHKHDDIAIGNVIGSNIFNIFFILGITGMIHPIDYNTEMNRD
                     IYVLIAGTLALMVFLFILTRHKLDRWKAFIMLAAYILYTGYLIKLG"
     gene            complement(66936..67892)
                     /locus_tag="PKM27_00160"
     CDS             complement(66936..67892)
                     /locus_tag="PKM27_00160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNR05690.1"
                     /translation="MKVLFKGLAWVLVELLVFAALFVASGALIVTGIQWISGNEYLFW
                     TSADLNEQTSPYTLLTSTFIPVLLSGTLAAGIAHHSIFKRPLSGLGFGYERSFFYFGQ
                     GWLWSFALVLPGFLILWFLGQIHLLPADWNGYYFAGFILFFIIQSAGEEVMTRAYLIP
                     MIEARFNTLAALVVSASIFALMHLGNEHFTWIGFLNILAGGTLMALFFIRYRNIWVCT
                     GLHAGWNFVQASLLDFNVSGIDVYSFIQFQDTGYPGLSGGDFGYEGSWIAWLIQVAGI
                     YLFYRWNRAELSVRFNRQPAPLTETTDAAKLWMPMQEENPGE"
     gene            complement(67892..68671)
                     /locus_tag="PKM27_00165"
     CDS             complement(67892..68671)
                     /locus_tag="PKM27_00165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNR05691.1"
                     /translation="MLDLSQGNLINLIITWMALAIAIFFRYVLMAGVFYYLFYVRFAG
                     RYHSQKVNLRPAKSKQVRMEVRYSFIASLLFAVMGTLAIYFWQRGGTLIYTDLSGWKA
                     WWLLPSLFLVLMIHETYYYWLHRWMHRPGVYRLVHRTHHESLTTTAWTSFSFHPVESV
                     LQALPVFILIFIMPLHPFTLIALFLIMSVTSVVNHLNVEIYPSGTHRHWLGKWWIGAT
                     HHALHHSRFHFNYGLYFTFWDHWMKTESPEFEALFEEKTKS"
     gene            complement(68749..69882)
                     /locus_tag="PKM27_00170"
     CDS             complement(68749..69882)
                     /locus_tag="PKM27_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNR05692.1"
                     /translation="MKQAQPYLTLLLALLIIQSAAYGQIDPKATRETKALFKNLKKLS
                     RKKVLFGHQHATEYGHGWSGEEDRSDVKSVTGSHPAVIGIDFMGLSGWPPEAIGRTME
                     NLKKNVLATFARGGVTTVAWHFMNPASRGGFYWRDSISAPAMSLIKPGGSHHEKYKGI
                     LDTVALFCKSVKNEKGRLAPMIFRPFHELDGGWFWWGKPHCTREEFIEVWTFTVRYLR
                     DSQQVHNLLYAFSPDCKFNTEEEFLERYPGNAWVDLVGMDNYRDMGRDTYNIEKAAGQ
                     LTILSRIAKANKKLAAFTETGLESIPNSEWWTATLLKTIRRPGIRLSYVLVWRNDVRS
                     PTHYYAPFPGQPSVPDFLKFYEDPYTLFEKDLRKIYRRRFLFF"
     gene            70226..73339
                     /locus_tag="PKM27_00175"
     CDS             70226..73339
                     /locus_tag="PKM27_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR05693.1"
                     /translation="MNLDLDPEGKEIVFDLLGDLYTLPVEGGTARLIRGGHAFEVQPR
                     FSPDGKKIIFTSDAGGGDNCWVMDRDGQNAKSITKEDFRLLNNGVWTPDGEFIIARKH
                     FTSTRSLGAGELWMYHVSGGKGLQLTPRKNDQQDLNEPWVSPDGRYVYYSEDMYPGGF
                     FQYNKDPNNQIFVIRRYDREKGTSETVTGGAGGAFRPQVSRDGKYLAFVKRVRTKSVL
                     YVRELETAREWPVFDQLSKDQQESWTIFGIYTGFAWSPDNRSIFIWANGKINKVNVAG
                     FNQSTEVPFTCQVSQRIYEAIRLKQNLNPGSFQVKAIRHLVTDPQGKYIVFHAAGHLW
                     RKELPAGLPQRLTRNGPFEFEPAFSPDGKYLVYVALGDSASSSLWKLNTNDLRSEKIS
                     EEQGNYRTPSFSPDGKQIVFVREGSSDLMGPAHTARPGIYIMDAAGGPARFVTPHGSQ
                     PRFNARGDRIYYQSGGGMNSTLNSCKPDGSDDRTQIKSTYGSQFTISPDEKWVAFIDL
                     HKVYIAALPQSGKIIDLGSGTADFPVRQVARDAGFNLHWSSDSRRLHYTLGNEYFSIP
                     LEDRFEFIAGKPESGFKMPESGIEIGLSLPMDKPEGRIAFTNARIITMKGDEVIANGT
                     LLVEGNLIKAIGKTGTVAIPADTRIIPCQGKTILPGFIDAHAHGNHFNTGLSPHQFWP
                     YYVNLAYGVTTMHDPSANTEWVFAQSELQKAGRIVGPRVFSTGTILYGADGNFKAVVN
                     NLEDARSAIRRTKAFGAFSVKSYNQPRRDQRQMIIQAAREMDIEVVPEGGSFFYHNTS
                     MILDGHTTIEHNMPVAELHEDIIQLWKHSKTAYTPTLIVSYGSVSGEYYWYQHTNVWE
                     KKRLLQFTPRSIVDTRSRHRTMLPEEEYENGHILVSKQLKKLNDAGVKINIGAHGQLQ
                     GLGAHWETWMMTQGGMDNHQALKTATINPAQSLGLDDWIGSLEPGKLADLLVLDKNPL
                     ENIYNTESIRYTMVNGRLYDAETMNETGNYDKHRKPFSWEMEKNAGYFPIIGESQEVG
                     KCLCGKH"
     gene            complement(73419..74060)
                     /locus_tag="PKM27_00180"
     CDS             complement(73419..74060)
                     /locus_tag="PKM27_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05694.1"
                     /translation="MIRFGLLFISILVLGSCAQISTFQTARTTPKNEGEIGLSLGIAG
                     ISESFGTDESFALPNLEFWGRYGLGQKTDLGIKISTGLSGVIDVKQQLVGDQTSKFAL
                     AVGPGLGIQGIVLDEVLFQAHLPVHLSYHPGERTAWYLTPRYVYQGVVGEGGLNYLGT
                     SLGVLFGRNIKFGLEYSFSKILDPVNDIPDEFDDEDFGANLYNIGFGVKFPIR"
     gene            complement(74148..80231)
                     /locus_tag="PKM27_00185"
     CDS             complement(74148..80231)
                     /locus_tag="PKM27_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HNR05695.1"
                     /translation="MIKFVYLFTLACVIVFFPSGKTAFRLQTFKEVMPVAESGSLAPD
                     AACDSITFLVQQLRTSPPSCCFQFILDNKLRNAITQIKLDISTATFKNIAVEVNAGWV
                     VSQTPQMDILLSHNSGFIPVGGASPLTFCLMGGNNPDALNISFNYTALGTSGSCNIPV
                     QVCSDPLPCDAAFSFNSISNCGNFLFTNQSTGPAPLTYSWNFGDPGSGSANTSTAQNP
                     NHQFSKCGDFIVCLKTTGPGCVDSICKTVAYFDLIKPVITCPANVTLRCNTNTAPAST
                     GTATATDNCTPSNAIVITYSDVVTGLMPCNGTIVRTWQAKDACGNTSSCNQTILIQDN
                     VPPTITCPSNLTVNTHPGTCYYTGALPSATAMDNCDQNLVVTYSLLASGSSILITPQT
                     QFPKGVNTITVFATDDCGNQSVNCNFTLTVVDNQPPSISCPANISLTGSLDAFGQCKV
                     VVNNIAPTVSDNCPMLNTTYVISGATVASGSQDASGTNFLQGLSTVTYTVTDMGGNTS
                     TCSFEIMVRCPRPKCDSISAFMVPFQAIPYACTFQFHVNNQASGITKIQIDLNTASFN
                     SAGIVSPFISVISPANRLTITHPSGFIPPGSFHAADLGINSVSNPHSAVIRFFYNNNL
                     STCDTNIVFNCPVDTTCGLSPSCATTTISLNTGVDQNGQLLPTGTFDPNWILIQSPYT
                     GLNLPRPAEVVAPYVSWHMQPNASTPCAQWISAFQGADKGSGPRYTYSNCFCVCKDSA
                     LITIRLSALADNEADFWLYDGSGNLLTTLLGIHPPGNNPSLAFLLPPDTSTTQLVLKK
                     GNYCIRAGVKNWTRVTGISVCGSVCGEGLIRDDCCKPESYITGIKYRDSLCTGQAYAG
                     QPVLPGWQFILCDAGGNTIDTVTTDASGSYVFGPLTPGNYSVKEINQPGWTLTYPSNN
                     LVPVALGPHEVQQANFGNCPPTTCDSISFWVESLRTVPPACCFRLHIDNKLKNKITQI
                     PLELNIASYNSITVDNLNGWFTSQSSLSNISLTHASGFIPTGSFTPLSWCINNGSNPD
                     TIKIKAEYLIGQTTVSCDTSFVFFCPPQPDTTCGPACLSDSISLSTGIDHSTGQFYPN
                     GSYDPYWTIVQTPYSNLNLPIPSLVTYTDPMFWDDQPNGSQPCSKWLTLYAFKYGHDQ
                     GIFSFQRCFCVCSDGAMIQIKLSVLADNSANFILCDSTGTTVSQLLSVPSSPGVPFHL
                     PTRDTIVNLILDKGRYCIKANVYNFDGSPTGVNVCGSIKGQGLGKDICCEPSAITGIK
                     YIDTICTGTSYTGSLQTLQGWQIILCNQQGVAIDTVTTDQNGSYIFSPLLPGLYTVKE
                     LNQPGWTPSLPATGMSSIFLDANQVAQVDFANCPPEVDSCCISQANFIQNAMNAVHLS
                     VVDSLCKVEINVLRLPPCDSIYFISWGDGQIDFGPFSTGMWMHSYGQSGNYTIQIPIV
                     EFDSNGTPCFDTLLKFTVDLHCQCECGRYTMNMSQNGTLIPVHCNDTITMGCPAPNPL
                     SFSGIFSCFGDSCSTPNIQWTLAGPATNLSGQVLPGNFQINLPGPLNTGYSTMTLQTI
                     CGMQICSCKIILFQPSCILSDSCTAVCNGTSWNQLNTSWIQDMVVYQGKLIVAGSFST
                     IGNPGIPVDNIASWDGTNWAPLPGGGLNAIVNDIEVHNGILYAGGAFTMAGNIPANKI
                     ASWNGVNWASIPQGGINGTMSNVDALLSTAWGLVVGGLFNSVGTSSIAANNIARWNPG
                     PGWATFGNGFNGPVFALSTFYGNIVAGGRFGNSAGGFNNVAIWVGYWTKVGGGGAVNI
                     PASPPNSGDGVHAIASYKNLLIVAGQFPSAVSTTVPNGNKTHHIAQWNGAYWTTLGIG
                     PNPSGSSVNNGNGIYALKVINNELYVGGQFTMVNNQNINLLAKWDGTTWFDLAHPATG
                     IVRSIALFSPNADSLCNLYTGGEVLFNQWKCSGVSIAHERQPISITIYPNPATNRVNI
                     QFNDLIRDEVRIDMYSLLGHQIRHLVLPKSRVEGTIGLETGDLAAGTYFVKVATARGS
                     IAKKIIVQSDR"
     gene            complement(80465..81220)
                     /locus_tag="PKM27_00190"
     CDS             complement(80465..81220)
                     /locus_tag="PKM27_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNR05696.1"
                     /translation="MTSYCHLSLSGPLATLEFGTPFHNALPSGVLEQMGAHLEKVSED
                     PEIRLVLIRSQGEKSFCAGADLSELLSIKEEEEGRRFFFQFARLILAIRNAPQIVISR
                     VQGKAVGGGLGLIAASDLSYATDQAEIRLAELINGIGPFVVGPAIKRKTGLSAFNHLT
                     LNPASWFTASWAMEHGLYNEVFHITEAMDEHIANKIDELSGYSRPALTEIKKMMWEGE
                     PEWDKWLLDRAGTSGRLLLTEEARQALQAIRRK"
     gene            complement(81416..82195)
                     /locus_tag="PKM27_00195"
     CDS             complement(81416..82195)
                     /locus_tag="PKM27_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HNR05697.1"
                     /translation="MKNCITIFLIFLTGMTSAQQAVSDEQILKAFQGLRVADVSDGMD
                     MVGLRDAGLMDTRIEALWKDIDSMKHIFCGIAVTARYVPTTKIVKNPMPKEEFQKWEG
                     NWYGKISTEPFVEFLKPGSVVVLDVQGDGDVGSVGSFNGLFWVSKGARGIISNGGIRD
                     TDELIKQQIPVYLDYMERGRGIRPGRNEIESVNKPVTIGGVLVRPGDVLVADGDGVIS
                     VPREHALQVAEFAHEILSKDKEGRKNLYIQLGKPLDQTVKQ"
     gene            complement(82507..84810)
                     /locus_tag="PKM27_00200"
     CDS             complement(82507..84810)
                     /locus_tag="PKM27_00200"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNR05698.1"
                     /translation="MNKILLYIFLFLIAGQRLSGQFLPEEYGITPDGRRLIRGNGEVK
                     GLYDDRIIRRVDLRFSQEDWPALLFENYPVKKDIPASLIMEGKTYPEVGVRYKGYSSF
                     HIARTKYKKSLNVTLDFVDESQNLMGYTTLNLHNGAWDPSFIKEVFFENLTRKYGPSL
                     KASFVDLYLNGTPWGLYSNIQALDGRFLRQWFPDNEGVSWRAESPGLVDQWKPGFTSL
                     NFMGEDSLVYQKHYTLKRSKMADPWKYLISLTRTLRNQDRPDSILSVLDVDRALWFLA
                     REIVFEDHNGYVFDGGMDYFLYLDNKSGRASPIQYDNNQIFFDQPQGNWNLFMREQDP
                     LFPLCQKLFSIPEFRQRYLAHVRTMTKDLFRQEVYLPMIERYDSLIRKVVAKDNKKLY
                     PYSSYIEAIEDLKIRMDHRKKYILTHPEVNREGLKITDTEYKFRNVAFQAPLAGQGVV
                     VNTRVSGPTPIDAVYIYVGTGVDGPFSRLRMEDDGKHQDGEALDGLFGAVLPAREGGE
                     FVRFYLEAIAADDFKTATYDPPAAENDVYCYRVGSDQRQNKNIVINEVMSANTLSVAD
                     QDHEFDDWIELHNNSEKEVDLSRWILTDNPANLVKYRIPEGIKISGKGYLVLWSDEDG
                     KQAGLHTNFKLSAFGEPLFLLDSTGSLVDSLTIPPMEEDIAYSRLPNGSGPFQKGSHS
                     FGKENKLSAMVQSAPVLKIVKVAGKAALSLSMHPPVPLAIQIFNEGGQPVYLNQFYRD
                     EHIDISQWPAGKYILRTGSSSQEIVIP"
     gene            complement(85000..87018)
                     /gene="uvrB"
                     /locus_tag="PKM27_00205"
     CDS             complement(85000..87018)
                     /gene="uvrB"
                     /locus_tag="PKM27_00205"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNR05699.1"
                     /translation="MPFKLTSKYEPTGDQPEAIRQLVRGVKSGDRHQVLLGVTGSGKT
                     FTIANVIQQVQKPTLILSHNKTLTAQLYGEFRDFFPENAVEYFVSYYDYYQPEAYISV
                     TDTFIEKDLSINEEIDKLRLRATSSLLSGRRDIILVASVSCIYGMGNPEDYKGGIIRI
                     EKGKRISRNAFLHQLVEALYSRTDLDLKRGNFRVRGDTVDIYLPYVDYGYRVIFFGDE
                     IESIEQIEIESGKRIEPMDNAAIFPANLYVAPRERLKYIIRDIEDELHEHEKFFQSEG
                     RYLEAKRIHERVNFDLEMIRELGYCSGVENYSRFFDGRAQGTRPFCLLDYFPEDYLMV
                     IDESHATLPQVRGMWGGDRARKLNLVNFGFRLPSAIDNRPLSFEEFEGLVNQVIYVSA
                     TPGDYELEKTMGEVVEQIVRPTGLLDPPIEVRPSLNQIDDLMEEISKRAESGERVLVT
                     TLTKRMAEELSKYLGRLNINCKYIHSEVQTMDRVEILRDLRLGNFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNARSLTQTAGRAARNVNGKVIFYADKITDSMQRTIDET
                     ERRRVKQMAYNEKYGIVPRSLAKTREEIMGQRSILDVRGPRNAYLEPQDRGLEAADPV
                     LEYAGRDQLEKLVAETEKKMKAAAKDLDFISAAQYRDELFALKKRLQA"
     gene            87258..87815
                     /locus_tag="PKM27_00210"
     CDS             87258..87815
                     /locus_tag="PKM27_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR05700.1"
                     /translation="MIEETDNELMYQVKSGRLDRLSLLFDRYQAPLFGFFRSLTRDAE
                     QSADLVQNVFYRILKYRNQFQGGQFRTWMFHIARNVHRDQYRRKNKAVKVSIDDYRDQ
                     LKEEVEADFDLHTREQHHQLRKAMDALPEDKKEILLLSKYQEKNYKEIGALLGCSEGT
                     VKVRVFRALHELRVLYHQLEKKEIL"
     gene            87812..88585
                     /locus_tag="PKM27_00215"
     CDS             87812..88585
                     /locus_tag="PKM27_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR05701.1"
                     /translation="MNETELKYLVGQYHSGRISQEEWQKLEEAIAQGRIDLSEIQVLN
                     ELSTSISRLEDPKPPVEIRGKVLEMIENENRLINREGIWINREWLIRTVLAAAAMLAG
                     ILAGYHLPWKSAPSGDVEALRGEVAALKETMLLTLIEKEAATDRLKAVSYSYDLDQGS
                     RKVIEALLTTLNEDNNINVRLAAVEALQRYGSEDWVRTQLVKSIGRQDSPLVQMALAE
                     CMVALNEKTSVQELQKLIHREETPEAVKDKLKGYIRQII"
     gene            88605..89522
                     /locus_tag="PKM27_00220"
     CDS             88605..89522
                     /locus_tag="PKM27_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05702.1"
                     /translation="MNKPVLIQAMLCLVLNSSQAQSFTEKISKTYSFEKKGSQNAVMV
                     FNINGNVTIEGTSGDQVLVEVEKTIRGKTDARLALGKEKIQLGVMDLADTLILYVKGT
                     GAEFSRNSKRKGPEAGGWGYQWERNWNGNNDNDNEDDRDEFEYTLNFKIKIPSSVHIM
                     ASTINNGDVVVSGTRGLVQTRNVNGHIRIKDITGGTLAHTINGNVDIDYASNPKQDCR
                     YYTLNGDIHVYFPTTVSGSMAFKSFNGDLYTNIEPLTPLPAVVTKKESEKGIKLKVEK
                     ERFRMRGDGPLLDVETFNGDAYLKEMINK"
     gene            89540..90346
                     /locus_tag="PKM27_00225"
     CDS             89540..90346
                     /locus_tag="PKM27_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNR05703.1"
                     /translation="MKNSTWIPLVLFMFAFIALAGAQSGNEFTIPLKDPAKRGKLYAH
                     LNAGSITVKGSARKDVLVKYSAVSDKEEKAESKEGLRRISGSSLDLTASEDDNNVKVV
                     SNSWNEEIKLEIEVPSGFDVKMHTYNSGDLNISNVQGEIDLTNYNGAIYASNIRGSVV
                     ATTYNDDIKVSLDEVKAGVPMAFSTYNGDIDVTLPAAAKATLKFKTDQGDIYTGFDVD
                     IKSSGPVKKEDSKAGVYKVMINEWRQGDINGGGAEFTFKNYNGDIYVRKK"
     gene            complement(90449..90838)
                     /locus_tag="PKM27_00230"
     CDS             complement(90449..90838)
                     /locus_tag="PKM27_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNR05704.1"
                     /translation="MRKDDLTYKVIGCAMKVHATLGSGYQEVIYQRALAIELAKQGIR
                     FIREYKMQVYYDGILIGERRVDFLIEGWLVLEIKAVAILEDSFLAQVMNYIEMFKFSY
                     GLLINFGCSSLEYKRVYNLKHPDNLVR"
     gene            complement(91036..92013)
                     /locus_tag="PKM27_00235"
     CDS             complement(91036..92013)
                     /locus_tag="PKM27_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNR05705.1"
                     /translation="MNKISGLRYALGFLLFPLFLSAQGWETMQTSGHCTARSESALAH
                     VKNKIYLLGSRGIRPVNVLNLKTKAWEALPSPPVELHHFQAVVYNGEIWVAGAFTGNY
                     PHELPVDHIYIYSPELKEWRKGPAIPKDRLRGSAGATVYKNKIYILGGTQDGHWDGHT
                     GWLDEYDPAKDTWTRLADAPHARDHVCIEVIDHKIYVAGGRRTTARTNNVINLKEASV
                     DVYDFKSGTWTTLPESSNLPTLRAGSPVVAHGHQLIVIGGESDTQVPAHSEVEVLDTK
                     TMKWNSFAKLNQGRHGTGAVRVKNKIYIVGGAGNRGGGPELNSVEVIGL"
     gene            complement(92052..>92596)
                     /locus_tag="PKM27_00240"
     CDS             complement(92052..>92596)
                     /locus_tag="PKM27_00240"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNR05706.1"
                     /translation="LDNAFGAELIRLFNEPLGYPSAMISRKIFDGQSSRFLGGGFWAG
                     YVAEELDQEPIIGLELEILPDPDPLALKVKVHVRALEVQSQVLSLSVALTETNIRDAQ
                     LTPTGVDTNYVHRHVLRSFLSAISGDPAGILPTNVKKTFEYSTTLSGNWKTGDLSLIA
                     FIHEPSPAFRVLQAAEAKID"
BASE COUNT        22831 a        24062 c        22323 g        23380 t
ORIGIN      
        1 taggattggt aggattggta ggattggtag gattggtagg attggtagga ttggtgggat
       61 tggtaggatt ggtgggattg gggggattat caggattggg ggtttggggc tgggatatgc
      121 accggccttc aattcatcgg ggtcaattgg aatcgcgatg taattgcatt tatccagttc
      181 ctgtctgttg ggcttctccg aagccgtata ttcttctctt attttttaaa tgaggtttgg
      241 cttctccgaa gccgatagga gtgcagagat caaatttttg catttctaat cccagatgtg
      301 tgcgaaatag gcactaatcg ccgcagtgag aacccaaatt gggaatgggc atcgaagatg
      361 cgtgcgttta caggacctat attgagtaag actccatgga aaccgggttt cgccgctgta
      421 tgtatttatt gactgaaact caggatatcc ttcgcaagcc tcatcctgca tggagaagcc
      481 cccaccccct aacctatcct tatcattatg ttgtattttt gagccattcg tcaacctgtc
      541 atttggaatg tcatgaaaaa agggttcttt atctttagcc tgtccgtttt tatggtttgc
      601 tgtgcccgaa aggactccac ggtaccggtc ctgggatttg ccgacgcctt ccaggacgta
      661 acgatcgagc aggccaaaaa cggcttcctg gatgccttga aagaacaggg atttgatgaa
      721 gaaaaaggaa cactgaaact cctgtaccgc aatgcccagg ggaatattcc caccctcacc
      781 cagatcatca actattttgt ggccagcaag gtgaccctga ttgcaagctg cccgaccttg
      841 tccaccatca ccgcagtcca gaacaacaag gacatcccca tattcatgat ggtctccccc
      901 atcccttccc tgatgaaggt aaacgatgcc caggaccagg ctccacggaa cctgttcggg
      961 gtgggtgaaa acctggatta catagacact tccttcctgc tcatacccac ggtgatcaaa
     1021 cccgggtcgg agccgctgac cgtaggcatg atctacaacc aggccgagcc ccagtcggtg
     1081 gacgccctgg agcacatcaa aaagctggcc gcggaccaca aggtcaacct gatctatcaa
     1141 tccatccatt cttccgcgga tgtccagctc gtatccgcat ccctgttgtc caaaaacatc
     1201 gatgtctttt ttgccaaccc cgataatatt gtatttgaat cctttgaaac cattgtggaa
     1261 gcctgtaacc aaaaaaacat tcccattttt accagcgaag ccggcctggt tcagcgggga
     1321 gccctggcgg cctacggggc ggacctatat caatggggtt accagtccgg ccttcaggcc
     1381 gcccaatacc tgaaaaccaa aagcacggag ggattgcatt gggaaatggt gaaagtgaga
     1441 aaaaacgtgt acaatcctgc ggtcgccgcc aaatacggga ttgagctgcc ttcaaacttc
     1501 caggcccttc aatgagccag ttctacctga ccgccttaca actggcttta tgcctttgcc
     1561 ccatggccct gggcatattt atctccatga aggtgttcaa cattccggat atcaccaccg
     1621 atggcagtta taccctcggt gcggtcatca cggccatcct gctgaccagg gaatggccgg
     1681 ccctggcggc aatgcctgcc gtgatgctta cgggtgcggt ggccggagtc tgcaccggcc
     1741 tgatccacac aaaactgaag atagacgcct tgttgtccgg catcctcgtg atgaccggtt
     1801 tgtattccat caacctgatc ctcctcggga ggtccaatct gccactcatc aaccttcaag
     1861 acctgtttac ctcccgctcc tttttcaccc atgagttgta caatcacctg ggcatatcca
     1921 tatttatagt tggcctgttg gtcctcctgc tgcattacct tttaaaaacg gattttggga
     1981 ttgccatgcg cgccacgggc aacaaccccg ttatgaccag ggccctgggc atcaacaacg
     2041 acctggtgaa gatcaccggc cttgccctgg ccaatgccct taccgcactg agtggatacc
     2101 tggtggccca gtttcagagc ttcacggata tcaacatggg cattggtatc gtgattaccg
     2161 gattgggttc tgtactgatc gccgatgcct taagggtctg gctcagggtc acgggtatcg
     2221 gatggcagct cttgtttgtc ctttcgggga gtttgatttt tcaaatggtc ctggccttca
     2281 cgcttgccat gggtatcgac cccaatttcc ttaaactggt gacggctctg tttgtgctcc
     2341 tgatcgtcgc cctgccccgg gtaagccggc cattaaaaaa cgattaaccc gccggaaagt
     2401 atgatctcca ttcaaaacct caccaagatc tacaaccccg gaagggaaaa cgaagtcacc
     2461 gccctcgatg aggtatccct tgaaatccgt aaaaatgaat tcgtggtcat catcggttca
     2521 aacggcagtg gaaaaaccac ccttttgaat atgatccagg gagcagaaag acccacctca
     2581 ggaagcatca cgatccaagg aatcgatgtc accagcctcc ccgactataa aagaagccgg
     2641 tggatcgccc gggtatttca aaaccccaat cagggtaccg ccccggactt gacgatcctg
     2701 gacaatttca ggctcgcggc tttgaggacc cagcccaaaa cgctgaaaat agggaccaca
     2761 gccactttta aaaaaaaggt ccgggataaa atcgccaccc tgggcatggg cctggaaaac
     2821 aaaaccagcc agtttatggg caatctttca ggaggccagc ggcaggccct gaccctgctc
     2881 atgagcgtga tggatgagac ggagatttta ttgctggatg aaccgaccgc agccctggat
     2941 ccgaggtctt cggcattgat attgaatctg gcggagaacc ttcaaaaaga actgggtatt
     3001 acgaccctgc tgatcaccca caatttaaag gatgcccatc attatggaaa ccgcttgctg
     3061 caattccggg aaggtaaaat caggcatgat gtccctgcgg gggaaaaagc ccgcttgtcc
     3121 atgcaggata tctttcgttg gtttgagtga agggataatc aaggttgaag tcgtcaaggc
     3181 atcagccggg ggtttacaga tggaaaaaaa cagtgattta gcccggaatg gtgggtcata
     3241 ttgcctgctc ggttcaggca tccccaactg caagtgggag aaggcggttt caaattattc
     3301 tgaattttgg ccgactgaaa caaactcttt tgcacaggac aagcacttca cctattggac
     3361 ttctatcctg cctctttgat cagccctctc aaaaagtgtg caaggctgtt gtagtgacac
     3421 agccatcccc tggcttcagc caggggtgtg gcagaaggaa gaaatagcgg gctttagcca
     3481 gcgtggcatg ttacctatcg atgctgaagt gaaaacatct gattcctgat ctgagctaaa
     3541 gcccgattca atacaaactc cccaaacccc agccagaagg ctggggtttg catcaagaat
     3601 tcatacacac gtaatctccc taaagggaaa ccatgaagat ttgattcaaa ccatttgtgc
     3661 caaagctaag cttcctttta aggcattttc ctcctataaa tccctttaaa tgcaaccgac
     3721 aatactacat ccgacggacc catgatttcc catagttgca gtactgattc atcctcattt
     3781 ttcttccagg ttattcgatt gtaatatttc gcgccgccct gtccttggac ccagccgctt
     3841 ttgaggatca tggaattcga ttctgcttta cctttgagtt ttaatatggt tccctgctta
     3901 tccaaccaga gctggttcca ggtggaatcc gatggatcat aataattgaa acttcttccg
     3961 gtacctcctg cggtgctccg ccagtgctcg ctcagaatac attggtcttc cagtttaaca
     4021 atcgaattct cccccagttt tttctccact gtgtcaaata ccatccactc tcccaaccaa
     4081 aaatcaaatt gacggtgttg cggggtgcaa caggcacaag gcgattgagc ggcgaggagg
     4141 gccgggacgg ccaaccacaa aaggcaaccc aaaaaacgga tccccatggt cccgggatca
     4201 ggagccaggg gtacggtaat gaaccgcgat gtgggacgct ttgtcaatat cttcaggtgt
     4261 aagcaatggg gtaatggtga tatccgccat gccggtggaa tccacgtgca gcgacatggc
     4321 caccccacta atttcatcct gcaattcaca gatgagatat aattcatccg gattcagggt
     4381 gtaatagaat gcctccaatt taccaccgag gtcattgatc attttttcga ccgcaatttt
     4441 acgggcagtt cctcccaact taaggacgcc accgaccccg gcagctgaat acttcgcacg
     4501 aactaaatac ttctttgaca tagaaaaacg ggtttatttt ttgcacttat ggatttaatt
     4561 aaaaaaacag ataataaaaa taagaaattc tgtggaaaca gcacattaga actcccttta
     4621 ttgggctttg aaattgagtg atatcggtgg gctctttctg ccacgaacct gccttcaaat
     4681 atagaagcag tcacttgaca ataggaaatt ggatgaaaaa tttatgagtc aaacgggcag
     4741 ttgtattata aattcagttc cccgtccttc ctcgcttttt acttcaatct ttccgccgtg
     4801 tgctttaatg acatcataag tgatagataa acctaaaccg gtgccttgtc cgggtggttt
     4861 agtagtgaaa aagggttgaa aaatcttatc gaccatttgc ggtggaatgc cgcttccatt
     4921 atcggcaata tggatgaaca attggtcggc actccttctg gtccggacga ccaccttggg
     4981 ttcaaaatcc ttaaagttct ttttttttct ttcgtttacg gcataaaggg cattgttgta
     5041 caaattcagc aatacccttc ccaggtcctg cgctacgatg tttattttgc cggctgaggg
     5101 atcaaaatcg gtttccagga tggcattgaa ttccttatcc tttgcccgga gcccgtgata
     5161 actcaatcta aggaattcat cgcacaacgg attgatgtcg accggttcct tctgtcctgt
     5221 atctgaccgg gaatgctgca acatcccttt gacgatggcc tctgcccgtt taccgtggta
     5281 atttattttt tctgaattgc ccgcaagatt atccgcaatc gcctggatct ctgccaggtt
     5341 ccctccattc acctcctgta tcaattcttt gatcaattca ttgtttactt cactgaaatt
     5401 attgacaaaa ttcaatgggt tttggatttc atgggcgatg cctgccgtaa gttctcccag
     5461 ggaagccatt ttggctgagt gaatcaactg gtcctgggta ttctttaatt ctgtcaatga
     5521 tttttccaga tgatcccttt gcgacgcgat ctcctccttc tgggtcatta cttctgccgt
     5581 gcgaagttgt acttgttctt ccagtcggga tttttctttt aaaatggatt tataccggta
     5641 gcggacaaat ccccctatgg cagaaccggc caaagcaagg tataaagcat atgcccacca
     5701 ggtccggtac caggggggca agaccctgaa gcggtagatc agaggttccg accagtttcc
     5761 ctccggactc ctggctttta gcctgaaggc atatttcccc tcggtcatat ttccgtagac
     5821 ggcggagggc atggctgtaa tcggactcca gtctttgtca taaccctcca gcatgtactg
     5881 atagcggatc atatggtttc tcgaaatctc ccttgctccg aattcaaaat ggatcttgtt
     5941 gtgcacgaaa ggtaaaacca gattttgcgg caatggataa tccggactaa tgctgtcaaa
     6001 acggatagaa ccaaatattc cccttaaact gtcccttgct tttccttcca gtggaatacc
     6061 gtagacctgg acttcctgtt gtgcaattaa ggtactgtcg cgtctttgcc cgccgaggtc
     6121 ataccaattg atggcatgat catctatttt aactgccttc aggtagggtt ctctcggagt
     6181 atcctttctt ttgagtgctt ttgggttcaa ttgaatgatc tgcccacctc ccatcagggc
     6241 ccatatattt cctttatccg gcaagacccc atgcggcaaa ttttttttct tctccaggat
     6301 ggccccatcc accatatcga tgaccgggaa gccggttttt gtattaaaca catcaaatac
     6361 cggatcatag gtctttaatg ttccgtttcc catatccatc tggcctgctc ctattaattt
     6421 ttgagttgcc tggccatcac tgcggacctt gtattgcaaa ctgctgaagc cctggtaact
     6481 gccgatccag agcacaccct gctcgtcttc caggatattg cataccaggt cctccggcaa
     6541 tccgtcctgc gctctgtaat tgattatacc ggaaccatca taccggccca atcccttttg
     6601 actagcaaac caaaaatttc ctttttggtc ctcgtcaata tcattgatat aattatcagc
     6661 aaggccctgg tctgtggtaa aattggtcat ggtttgtccg tcaaaccggg ataccccacc
     6721 ggctgtgcca aaccagaaat ggcctcttga atcctgaagg atactgagca ccatattatg
     6781 tgccagtcct tgtgctgtgg aataacatac catttcttta ccattaaaac ggcaggcccc
     6841 acgatcaaaa gtgccgatcc agatattccc gtcccggtct tccgttaaaa tggacacata
     6901 ttcagaggat aagccctgtt tggtagaata cgtggtgatc cgtttaccat caaaccggtt
     6961 caggccaccc tctccctttg tcccaaacca aatattgccc ttcctatcct cgaccagtcc
     7021 gttgacataa ttgctgatta agccttgctt cgtggtatag taagtgaagg attgaccatc
     7081 atagttccca agaccccctc catctgaaac aaaccagatt ttgcctgtcc ggtcttcaat
     7141 caaggaataa cactgttgag ggggtaatcc ttgcccggga ctaagcacct gtatggcatc
     7201 tccgttgaac cggctcaagc cgttgcccat ggtggcaaac caaaggtttc catcctggtc
     7261 gtttaccata tccgatatgg tatttcctgc caaaccctct tccgtggtgt atttaataaa
     7321 tttttctcct gtatagcgga ccactcctga tcccagcgtt gaaaaccaaa gatgcccgtg
     7381 gacatcttct tcgatggccg ttacaggaat aacttccttc tcagcaccca ggtttaccag
     7441 ggtaaacgta tcgttatggt aacgccccaa tccagcgtcc agtaatccaa accatagggc
     7501 gccttttgaa tcttcaaata tactgtagat ggtggttgcg gtaaatgatt ccggtgaaat
     7561 ccgtatgaat tgggcattcc tgtatttgta gattccttca tttaagaagc caaaccaaag
     7621 atttccttcc ctgtcctccg ccatagctgt cacataatta gagggcaggc cattggcagt
     7681 atctaagatt gaaaaattca gcccatcata ccggctgacc cctttgggga aggttataaa
     7741 ccacaattcc ccctttttat caagtaaaat ttgatggatt gcatttccgg aaagcccgtc
     7801 cctggtcgta taattggtaa actttcgtcc atcgaacctg gataatcccc cttcatgtcc
     7861 accgaaccaa agatgaccat ttttgtcctc tgcgatcgac atgatcgcca cgctggccaa
     7921 cccatttgaa agaccaaaat tggtaaaacc cattccgtca taacagctta cgcctccccc
     7981 ccctgttcca aaccaaagcc ttcccctgct gtccaggaaa ccacaggtca ccccatccag
     8041 ggccaggcca tcctttgtat ttaaattcgt aaagtgcacc agtggcggca aagcctcaag
     8101 cacttccgga ggagaatagg aggaccctgt attcttccaa aaggattggt ttttgatgcc
     8161 gattcctttc tttggcaggt tgagtgtcac aacccgtggg gaaggggcat cggttaaacg
     8221 ggtcctcgcc ggagggggca gggtatccaa aaaatgcact accggcggca aaaaagaaga
     8281 ttgagcgatt ttgaccccag tggtttggtt ttcagaacag gaatatccag aaaggatcac
     8341 ggataggaca ccggcaaaaa gtactgcctt atctctgtat gtccagaaaa agtaaatcat
     8401 actaatcatt catagaatgc aggcgtatat agattggaat tccaaagagg gcgtattcgc
     8461 tttaaaccgc catccggatt atagatcttt tcgcacgacc tatgcttgga aagataagaa
     8521 taatctgcag ccctccccgt cttctgctca agtttattct aaccttcttg tacagtgagg
     8581 gggtgactta ggtaaggggc tgactggccg gtaaacgaat aacaaattcc gatccccggc
     8641 cctcctccga aatcacctcc agggttccac cagacggacc ttcgatttta ttaactacaa
     8701 tagtaccgcc aggcggacct tcgattttat taactacaat agtaccgccg tgccccttgg
     8761 tcacgatgtc ataagacagg gacaaaccca ggcccgttcc ttccccggca ggcttagtcg
     8821 taaaaaaagg ttggaagatc ttttcacgga tggattccgg gatacccgtt ccattgtcct
     8881 taatgaaaat ttcaagccgg ttttccacaa gcctggtacc aacagtcacc aggggtttgt
     8941 actccggttc tccaatttgg gtcagagggt ttaccgtacc aggggtttgc gcggaacggc
     9001 ttcgttcggc gcaagcataa aaagcattat tcaccaggtt cagcaatacc cgaccgatat
     9061 cctgggtaac catttttatc taaggaaggt atccggctgc tttcaactgg ataagggagg
     9121 acctcccggg atcataaaag gcctgcgggc ccccttatat ttcgtttttg tggctttaaa
     9181 cttcgccgtc atgtattcac caagatgaat ggaccccgac atcaccggta atcctgcctc
     9241 gtcgtttttg actaccccgc taaacaaata ggtaatcgaa tcgcccggcc tccgcaatac
     9301 gcttcgcatt ttgatctgat caccttctac cagtccaaga acttccgatt ctgaaaaatc
     9361 agcctggtgg gttccctgga tccagtttcc atcctgttcg atatacaggg tatgtttggc
     9421 tatgctgctg aaatattgaa tctcgagatc ccatggcccg tcaatctgta aggatggact
     9481 ggccatttga tctgattttg gacttcgttc agcggagaga atggcataaa gcctgtcagc
     9541 caccacgggc acctgttctt gcctcatctg gctgggcacg atggaaataa aagaggtccc
     9601 ttcaccggat cctccaccga tggcaatccg tggtttgctg cgtgcggtga tttcagccac
     9661 ttcctcgccg gtgatatgaa attgattggg gttccaggat atacgtaatg ccggtgcccg
     9721 gttggaaagg ccggtgggca tgcggatttc ggtgctgatg ccggacaggg atttgaactt
     9781 tgaagcgatc gtgttcagtc tttccagcca tatattccat tccgcttcat ggttgcgttc
     9841 ggtccaggcc tcgaccgcgg ccagcatgcc catgatttct tccttgccca ccttgttgtc
     9901 ccggccaggc ccatggtgcg gtgcgctcgc ctgccaggcc gacatcagca aatcttttgg
     9961 cccgagtagg atgccggcac attggggacc acaaatcgcc ttgcctccac tataagccac
    10021 cgctgtggct cccctttcca ggtgaatggg gggaatggtc aggttttcag cggcggcgtc
    10081 agcgaggatg ggaacgctat ggggtttcgc gatcgaggcg atgacttcaa gggaaagttc
    10141 tttcccggta tcgttggggg ggcccgtcat aatatagatc atggcggtcc ggggattgat
    10201 ggccctttcc aagtcttccg gactgttgac ttcaatgatg gacacgccca tattacggat
    10261 ggcatgatcg tatacattcc tcgaactgcg gggtatgatc acttctgttt tttcaaatcc
    10321 cgtcaggtcg ggtatcctca acaatttttc cggatttcca ccggtgaggc aggccgcggt
    10381 aaaatgtttc atggcggctg cacagcccga ggtgacgatg ccccattccg acttcgtcag
    10441 ttcggccagc cttttaccca cccccatggc caattcgtca tattgaacaa agtagcctga
    10501 ggcggcttcc atcgcccggg tcacttccgg caattccatg gagccgccaa tgatggtaaa
    10561 ggtccccctg caattgatga tgggttcgac gcccacagag gcatagatcg aatcgccccc
    10621 ggcaaccccc tcatcagggc ctgtcgagag gttctcggat aagggtatgg ctgcaaaggg
    10681 tgccgcagtg aggtttttga gcaaatcgcg tctattcata ccatgaagtt acagacaggg
    10741 ggtgacttaa gtgaggggat cactgtcagc cgggtaattt gattatgaat tccgtccctt
    10801 cgccttcctg cgtcacgacc tcaagcgtcc caccatgccc tttggtgacc atatcatagg
    10861 ccaaagacaa acccagcccc gtgccttccc cggtcggttt ggtggtaaaa aagggctgaa
    10921 aaatcttatc caggaattcc cggggaatgc cctggccatt gtccttgata cggatttcag
    10981 cgaatgctcc cgttccgctt tttatatttt tagtgctcac caaaacagtt ggcctgaatt
    11041 ccttctcccc ttgcctggct ttgcatttgg ccgtgacgga ataaaaagcg ttattgaaaa
    11101 gattgagcag cacccttccg atctcctggg ggaccacaga aattctagga agatcaggat
    11161 ccaggtcgag cctgaattcc gcctggaagg ttttgtcctt ggccttcagc ccatggtaac
    11221 tcaatcggag atattcatcg caaagcgcat tgaggtcggt ggactctttc tgcccggtcg
    11281 cattccggga atgctgaagc atgctcttga caatggagtc ggcgcgtttt ccgtggtagt
    11341 tgattttttg ttcgttttcc gtgatgtctt ttgcgagacg ctgcactgct tcgtaattcg
    11401 atttcaggat ttcttcattc atttcttcaa tcagctccgc attgacttcc gaaaaattgt
    11461 tgacgaaatt cagggggttc tggatctcat gggcaatacc ggcggtgagt tccccaaggc
    11521 tcgccatttt ttcagagtgg atgagctggg cctgggtggc cttcagggca tcgtgggcct
    11581 tgcccaattc gagataagcc ttttcaattt gccgggcctg ctccagttcc ttggcctggg
    11641 accttgcccg ttctttctca atcaaccgca tccggaccac ccggaacatt ccataaccgg
    11701 cagcgagaag ggcaaggcca tagaggatat aggccgtcca ggtcttccac cagggaggac
    11761 ggatgataat cttcagggaa accccctctt cattccagac gccgttgtta ttggctgcct
    11821 ttacacggaa gacatattta cccggaggaa gtttcgggaa aaaagcgaca cgttgattgc
    11881 cgatatcccg ccattggtcc tcgtagtttt ccattttata ggcatagcgg ttttttgaag
    11941 gattggcata atgaaggcct gtaaattcca gggtaaggtt gttttggttg tattccagaa
    12001 tgatttcacc ggcttcatag atggcatgtt tcagagggga atctccgccg ggaagcagtg
    12061 accggttgaa taattttagg tcggtaagat aaaccctggg cggtacggat ttttgcatca
    12121 gatcggaggg attaaaaacc gtaatgccat tggatccgcc aaaaaccata tatccggtgg
    12181 aagttttgag gcaggatccg tctgcaaaca actgactttg gatgccgtcc gccagggaat
    12241 aattatagaa cctttcctct tccaggtcca gccggctgag tccttcaaaa gtactcagcc
    12301 acagcgcttt gtttttttca tctcccagga tcccttgtat gctcatggaa ggcaggccgt
    12361 catcgagggt ataattccgg attgttttat tgaccgcatc gtagcgggca agtcccccct
    12421 gccaggtacc cacccaggtg ttcccggaag aatcttcata aatggaattg atgtcctggg
    12481 agatgaatcg gtccagggct tcgccgccat ccccatgcct gtgaatagag tcggtttgat
    12541 agtcataaaa aaacaaacca ttgttggtcg tcagccacaa accctgatgt ttccggcttt
    12601 ccagtgctcc ggtgacttcg ttgctgctgg catccccgcc gggtgcggcg ctcagatcat
    12661 atctccggaa actgtccctg cctgctgccc ttttataaag tccatgtact gtgcacagcc
    12721 atatattcat cttttcatct tccaggaaat gattgacagt gggcagatga gcctctgaac
    12781 ccattctttc caaacccttt aaatgcaatg gggttgttcg ttttgagcga atggaaatat
    12841 taaacaatcc attgaaggtt ccaaccatca actcacccgg gctgtcctcc cggattgaaa
    12901 atatcgcttc gctgggaata cctaaatcat aaaaagtcag cttttgatta atcccggact
    12961 tcagatccag gtgattcaat ccagcgacgt ctattgctcc cccggttgtg atccagagtg
    13021 taccatcata agattcgatg attttgtttg cccaccccgt ggtgaatgcc tggactccgg
    13081 gactggatgg ggtataactt ttaaattcca gcttatccga taaaatatac aagccattcc
    13141 ggtgcgtccc gatccacaga ttgccaaaat catcaaattc aaggtcacgg accgtgttgg
    13201 tcgccgggaa ttccatggct tggtccgaaa agaaggaaaa ttgttctgac cccttgttaa
    13261 aataaataag tccctggttg gaccctatcc agagattgtt cttcaggtct gtcgagatcc
    13321 ggctgaaata ataatcggtt tgcggcttgg gcgaattatg gatccggtac ctccggtcct
    13381 ttgggttaaa atccataatg ctccccttcg agctggtgat ccagatatgg ccatcccggc
    13441 cgggatcgag cgcaacggga ataaatccgc ttcgatcggc ctcctcccga gtaaatatcc
    13501 gggtcaatct ccgatccttg tcaattttga ataccccgtt gtaggagatc agccaaatat
    13561 tgccttcctg gtctttatta atatggatga cattctggat ttccaggctg tccggcaccg
    13621 gaaaggcctt tatggtcgaa tcccggacat ccttgaaaag caggccaccc ctgatggttt
    13681 cctggaaatt ggtcgtgacc ccgaaataaa gtcttcccga atcatcctcg caaatggacg
    13741 taatccccaa ttccacattg ctggcatggt caatcaggtg ggaaaattca aattgcctga
    13801 aagccctggt agcgggatca aaccggttga gataagcagg tccggtaccg gcccagatca
    13861 tgccctcacg atcttcatat aaggaccaga cgacaaggcc cttgatggaa ttcggatcat
    13921 tggggttgtt taaaaaacgt ctgaactcat atccatccca acgggccaga ccgttgatgg
    13981 tacccgccca gatatagccg aaacgatcgg ccaggagctc accgacttcg gcactgggca
    14041 ggccgtcgag ttcagtgtat tgcctgagat ggccgggact gatttgggct gttaaaggga
    14101 tggataagca gcaaaggata aaaaggaaga ccgtttgaat ttttccaacc atttgaaacc
    14161 cggccccttt catggcttta aattacgaag gtttgggaga tcgtgatcgg ggagaccgtt
    14221 tatcagttca acagtttatc ggtttaccag ttcaacagtt tttcggatta ccaggtcagt
    14281 gcttgctttc cagataatgc tttttcagaa tgtcctctcc gaaatcattt cccgtttccc
    14341 tcagaatggc atggatgtga ttgccgtcgt tctggaaacc tacgttgtca tattcgatga
    14401 gaaagtcagg gccatggagg atataataat gcggcttatt gatttccagg ctgcccaccc
    14461 aggcgaaata tattttgttc aggccggttt tgagtattct ggaatagagg tcgtgggcaa
    14521 aagcatgttc aaaattgtgg atgtattcct ggatcaggat ttccaggagg gaccgctggg
    14581 cttcattgaa ctcccccgcc gcgatgccat aatattcaga gatccgctgg ctgctgttgg
    14641 gattggtgat gatatcgccc gggacttccc gtttaaggat ggctttcgat tgttgggact
    14701 ccgaaagcat gttgaccagc ataaagccat agtcttcttc tttactgagt acccgccagc
    14761 ctgcatattt ggaagatttg atttcagacg gatcggtgcc cacgaacagg ggggacaggg
    14821 aaaggtgttc ccccttcacc gtgcaggata aagcgatatg atgtcctccg aaactcaggc
    14881 cccaggcttc cttttcctgt ggctggcccc ataccgatat ataatagttg ccgtgttccc
    14941 atttgaggtt ccggatttgg gtaaaggttt ccctgtttat ttttccctga tcgaaagcct
    15001 cctggtacag gacattcagg atatcgtcca gttgcatgat gctggtcaat ttgaggtaac
    15061 cctgcgaact caataatgaa gacaggaccc ggtggaaggc catcctgctt tgatcggaca
    15121 ggtctccata acgaatgccc ggccgcggca ccaggcccac gggtaggttg gtccattttt
    15181 tccgcagggt atcctcaaag gaataagccg cttttttcaa ttcgtccgga ctgagcgttt
    15241 ggacaaaatg ccgtgttgaa ctgatcaatt gcttaccaga ttgggctcct gcaaaagctg
    15301 cctggatggc cataaaaccg gccaggagga atcgtttcat ttttgaaaat tgactttgaa
    15361 ggtagggata tttcgcaagg tttctctctg catttccgtc gaggtggttg agctaaaagt
    15421 ctttggtctg tggctaaagc caaattgatc tctttggttt ttaccccggg ctaaagccac
    15481 ggggtttaga aaatgtgcca accattttcc caaagtgatt gccacttgct gcgcttcagg
    15541 cgggcgctaa gtgacaacca ctggccggtg gaggacacct tagaattatc ccgaggcttc
    15601 ccaaagtgat tgccactggc aacgcccaac tgttgggtcg aagtttggct tcggcctggt
    15661 acgtacgttg tagttttaca tttctacaag aaagcaaggg ggtcggagct aagctcctcc
    15721 ccaataaaaa cgttgggtcg aagcttggct tcggtccggt acccttactg tagtttcgtt
    15781 cccgaggcta tttggttttt accccgggct aaagccacgg ggtttgtggc ttcccagggt
    15841 gattgcacct tgcggtaaac ggtggccttc acctagggtt agcaagtgcg taagccggtg
    15901 gaggacacct tagaattatc ccgaggcttc cctaagtgat tgccacttgc tgcgcttcag
    15961 gcgagcgtta agtgacaacc actggcaacg cccaactgtt cggtcgaagt ttggcttagg
    16021 cctggtacgt acgttgtagt tttacatttc taggggaaag caagggtggt cggagctaag
    16081 ctcctcccca acgctaccct aagtgattgc cacttgctgc gctccaggcg ggcgctaagt
    16141 gacaaccact ggccggtgga ggacacctta gaattatccc gaggcttccc aaagtgattg
    16201 ccacttgctg cgcttcaggc gggagctaag tgacaaccac tggcaacgcc caactgttgg
    16261 gtcgaagttt ggcttcggca tggtacatac gttgtagttt tacatttcta caagaaagta
    16321 agggggtcgg agctaagctc ctccccaacg cagccctaag tgattgccag tgctgcacct
    16381 caagtgggtc ctaaaggatc cataattcgt tccagcttcc ctaagtgatt gccacttgct
    16441 gcgcttcagg cgggcgccaa gtgacaacca ctggcaacgc ccaactgttg ggtcgaagtt
    16501 tggcttcggc gcaagcgcta agtgacaacc actggcaacg cccaactgtt gggtcgaagt
    16561 ttggcttcgg cgcaagcgct aagtgacaac cactgggcgg gcgcgcgaaa aaaaggccaa
    16621 acccgcatga cgcaaggatt ggcctcataa attggttcaa acaattttat tgcatggcgc
    16681 tggcgggggg aaggggggct ggaaccgcgt tctttatagg cgggatcgtc ctcaggtaag
    16741 caaatatggc ccggatctca tcatccgtgt aatgacgggt ggcttcgttc cagggcatgg
    16801 ggggaaggat ggtccggccg gcagcaagac ctttggattt cccttcccgg agtgccagga
    16861 ataaattttc aacggtccag gctccggtgc ctgtagtgga atccggtgtc aggttagccg
    16921 tataagaaac cccccagggt ccgacccatt cggtcagatc catcgtgacg gcgtagccct
    16981 ttttctccat ggccttccgg tcaacatcta ttttaggcat ggcggcggga tggcccgcaa
    17041 gcatcatgga gctgtcgagt tcaggtccgt gcggccccat tttttttggg gtgtggcagt
    17101 cgttgcatcc cccgacggtg accaggtgat ggccccattc ggcttcggtc gcataaccgt
    17161 gaaagtcggc tttggcggcc atggcatcgt ctgccatcgc gtcggcggcc ggtgcgggtt
    17221 tgttctcggc gcattggtac agcataaaca ggcttagtgc caggaaggca gaaaccattt
    17281 ttgttttcat gattttaggt tttattgaga tttatgaaat gagattttgg ttgatgcacc
    17341 acgggggagg gggatccttt gggatttaat tcaaaacgaa gctaatcaat tttatgggga
    17401 gaaagaaata gcttaagcag aattttttag cagtgttgga aaatttcatt ttaaaaaact
    17461 gtcgcggaag aaggctaaat tttccatggc tgttccccga ttcaaaaaat atgaagtcaa
    17521 acttggacga cagggtaaaa tggttatctt gagcaattga aaacgaccgg acgatttgac
    17581 cagacctttt tttaaatcag gtttgtaaaa taagtcgacc gctaaatcaa aagtcaaatg
    17641 ccccagaccc gccgccttgt tgccatcatg tttaccgata tcgtgggtta taccgccctc
    17701 atgggtaacg atgagcaaaa agcctttcaa ttgctcaaga aaaaccgtga tctgcaaaaa
    17761 ccggtcatcg aggaattcgg aggcacctgg atcaaggaat taggggatgg ggtgatggcc
    17821 agtttcaata ccgtcaccga cgccgtacag gcggctatcc ggatccagga agcctgcaac
    17881 cgggcaaagg attttctttt gcgcatcggc attcaccacg gtgaggtggt ttttgaagag
    17941 caggacgctt ttggcgacgt ggtaaacatc gcttcccgac ttcaagccct cgccctcccg
    18001 gggtcgatct ggatcagcga atcggtccat cacaatgtgg tcaacaaacc cggaatcaac
    18061 tcgcggtatg taaaacagga agtgcttaag aacgtgaggg agccggtcaa tatctatgaa
    18121 atccttaccg gtgaccagga tattcctgag gatcaccttg tttccaagaa aacgaaatcc
    18181 attcttttcc ccgaatacga ctgtgatgtc tacatcagct accgggcggc cgataataaa
    18241 cagaccggaa ccacttcgtc cccgggttgg gtatctgaac tcaacgaaaa actcaggcag
    18301 gagctcgagg ctaccctgaa agacaagctg accgtttgtt ttggtcccag cccggatgtc
    18361 gaaaacaaaa ccggtgaaac ccggaaagcc atcaattcgc tgatcttcat cccggtcatt
    18421 tcccagacct attgtgatcc ctcatcccag ccctggaact ccgaattcct gaaattcaag
    18481 gaggaggccg ctgcggacaa gctgggcctg aatatccggc tgcccaatgg caacagcgcc
    18541 tgccgcatcc ttcctgtaaa catccacgaa ctcgatttcg aagacctccg cctgctggaa
    18601 aaagaactgt ccggggattt gcgctctatt gattttatat accgggatca cggcatcaac
    18661 cgccccctgc ggcccgctga tgacaacctc catccggaag ccggcaggat attgtaccga
    18721 aaccagatca ataaactggc caatgccatc aaggagatta tccatggcat taaggcccag
    18781 gccaaatcga ctgacccggc cgataccggt cccagcatca cctcccggac cttacccttg
    18841 aaaaatttca tcacttccct gcccagttcg atccaggtgc aggtgattga ccgggctaaa
    18901 cccaccgttt tccttgcctg gacctctgcg gaccttaaag agcaaaggga agaaatggtc
    18961 atcatccttc agaaagccgg attcaatgta ttgcctacca cggattgccc ttcggatgat
    19021 gaattgttca aacaaaaagt ggctgaaagc ctggaaaaat cttcctgttc ccttcatatc
    19081 ctgagcgggg aattcggccg ccgcttcgaa tcggatgaag aaacttcctt cccacagtac
    19141 cagtttatgg aagcaaaaca acggatggaa ggaaacccgg attttaattc gtttatctgg
    19201 atccatcccg cgagcagcaa tctcacggta aaacccgccc aacaaaattt catcaaatac
    19261 atccgcaacc acatcacccg gaacatgatg ttttccaacg cggccgggcc catgcaactg
    19321 gtggatgaca tgagggtcgt gatgatgaaa caagactcgg aaatctacga tgccaaggat
    19381 accgacatct tcttcatttt caaccaacag gacgaagcgg aagccaaagt cattaccgac
    19441 cacatcagcc tggagtatcc ggtcgaactc atgaacatca tgccggacag tccggaccaa
    19501 taccgcgaga tctcgtccca gcagataccc aaaagcaaat tggccgtggt ttattttaaa
    19561 tatgccgccg actgggccct gcctttcatc aagcagatct ggaaagaagt cgggggagct
    19621 tcttcgccca cccccctgtt cctggtcggg gaagacgacc ccaggaccaa tctggcccgc
    19681 aatttcaaag cgcctaaagt agtgtccagc gtcgttccca aagaacaaat cccctcagaa
    19741 gtaaaaaaag tttataccac cgtcatcgaa ctcaaataag atgttcgaag agtaccagat
    19801 ctgcccttac accggattgc gctctttcac ggaggaggag tccctgtatt tcaaaggaag
    19861 ggaccaggat attgaacaag ccaccctgca gcttcagcgc aataaatttc tcatgcttac
    19921 cggggcatcc ggggatggaa aatcctccct ggtctatgcg gggatcgtgc ccaacgcccg
    19981 cgccggattc ctgaagtcta cctataccca gtggcgggta gccgaattca ggcctgaacg
    20041 caatcctttc ggtaatttat gcaaggcgct tacccgtcag ctggacatcc ccaatcccgc
    20101 catcacggaa tccgaattgc atcatggctt ttccgccctg atcgatttgt ataaaaactc
    20161 ggatgcctac ctcgactccg gctccatcga atggcaacaa gccgacgaca aggaaaaggc
    20221 ggcactgaaa cggagagcgg gcaacctgat gatcatcgtg gatcagtttg aggaattttt
    20281 caccaacccg gaaaactatc atcagggagt cccttccaag gattcaaacc tggttctcaa
    20341 cctgcttctt gaaacggcaa ggattgccct cgatgaaaac ctgcccgttt atgtggtctt
    20401 taccatgagg tcggatttta ttggtcaatg cgcggctttc cgttccctgc ctgaattcat
    20461 cggtttttcc caatattttg taccccggct caaccggctc cagctccagc aggtgatcga
    20521 agagccggca gaactcagcg gcaaccggat ttcgagaagg ctcacggaac gcctgatcca
    20581 cgacattgcc gaaggggtgg accagctccc catcctgcaa catgccctca accagatctg
    20641 gcacgcggcc aataaaggca aggaagagat ggacctgatc cattacgcca tggtgggcgg
    20701 gatgccggcc agtgacttgc cggacgatca aaccggtatg tttaaatcgt ggtttgacca
    20761 gctgcctgaa cacatccggg cctgttacca ccggcccagc cttcagaatg tgctggacac
    20821 ccatgccaat aaactgtatg agtccgccga agaatattac accgccaaga ccggcaaacc
    20881 ccttccggac gaaacctcca aagccatcat ccggaccgct ttcagctgcc ttaccaaaat
    20941 cgaccaaagc cgggcggtgc gaaaccggat gaccctgaac gaaatctggc agatccttaa
    21001 ccggaaggaa gtcgaactgg aaacggtagg acaggtgctg aatattttcc gggagccggg
    21061 caacaccttt atacgtccct tcatgaccga agacccggag accgcaaacc tgggtggtga
    21121 aaatgtgctg gacattacgc acgaaagtct catccggaac tgggactccc tcaaaacctg
    21181 ggccaaggca gaatatgaca gctaccaggt ctcccaggat tttgagcaac aattggggcg
    21241 ttgggtgcaa agcggtaaat caaataattt cctgttatcc ataggccccc tgacttattt
    21301 tgaaaactgg tttaaacagg taaagcccaa cagctggtgg atcgcccgct acctaccgga
    21361 ggaagaggat gccggcaaga aaaaaactaa agccgaagcc ctgattgcca atgctaacga
    21421 attcctggcc agaagcgccc agaaacatgt agtcacccgt acggtcatga aactgggtcc
    21481 tcgtaaaatt gcagcggccc tgggattgtt ggtcatcctt accctgagtt cctttgcctt
    21541 atccaactac ctcagcaagc ggaacagcgc ggttttgaaa accattaaaa aggaatccct
    21601 ttccctggca aacaatccca aactgggcct ggagttttat gtccccaacc tgacccaact
    21661 catgcaggag ggcagcctta cccttcccga aatcatggaa tccatcaaag atccctggca
    21721 aaaaatccat gtaagcacgg gaatcgccaa gctgctccaa atgcagggga aaaacgaacc
    21781 cgcggccgaa atgacccggg ctttttccat ttcggacagt ctgctaacat tatttgacac
    21841 cacagccgat gaccaggttc ctctcaacag tctgcttaat gaacaatata acctgaaaat
    21901 cgccctgggt ttcgcccttc tgcactcgac cgattccaca tgggccggac tggccaggaa
    21961 gaatgccgtg cgatctggct tatgggccaa aaggatcctg gcagatcctc cgggggattt
    22021 taatgaaatg cgtttgctca tgctgggact ggaagacgcc ctccattaca gggtcctgat
    22081 ccctgaggat atccatgccc ttatcggtca gttatctcct ttttcaccca accaagcctc
    22141 cgaaaggtta agccggttgt ttgaccggga ccaactccag gtgcggggta cccaggatta
    22201 tggttatatg ttcaatgccc tgtttcagga cctggcttat ctctatgcgg ccgccggaga
    22261 tgaggagaag gccctatggt gcatcgattc gctcctgtct tatccccagg gatacctcga
    22321 aaacaattat gaaaccatgg cggacaatgc gaccaatatc gcggcccatt ttttcactta
    22381 tggccatttg gacgccctgg agcgcttcat ccggggatat tcccttaaaa aaggaatccc
    22441 ttcccatgaa ttttacaacc ggatgatctc ccgcagcctg ctggatcata cgtcaactaa
    22501 tacctataac tattataatg tggtgggcca ggcttatacc aatatgaacc tgaagtttag
    22561 cggggatgat tttatgtatt ttgcattccg gaagtaccgg gaagccatcc agcaaatcgc
    22621 cgatcccgat cagcgatccc tgaacctggc cgttgctttt aaaaatgaag ccattttgtt
    22681 gtccttccgg aatgcgcttc ggggaagccc cttcccccag gttcggctcg atagcctgtt
    22741 cgacctctcc ttaaatcact acaggcaggt cagggcggaa tatctgtcac aggtcaatcc
    22801 tgtggtcagc agttcggaag tggaccggat caacgtaccc aactcggagc aatatctctt
    22861 tcccgattat tttgtgccct tccacccgtt tgaacccagg ggttacctgt ttcagttcaa
    22921 tgatccggcc ttcgtggatt atatcctgag gcaggatctt tttaaagaac tgtaccgcac
    22981 cgaccaggac atccggcccg tccagcggtg gctgaatttt tacaatgccg tatccatttc
    23041 cagggattat tttttaaaag aagccattcc cgcagatctc atgactgggc tggcggaaaa
    23101 gctggaagcc ggcggttttg aacaacgaaa cgacctgaac ctcctttacc tccatctggg
    23161 caataccctt tccgcgcaga acaagaaaga ggaagcgtgg agatattatc agaaaatcca
    23221 gcctcagtgg gtcctcaatg cctttcaata caagggaatg aattttctga atacctattc
    23281 cctggaacag tacggaatcg ctatcggcca cctcttttca tcacaacacg atcaaaaagc
    23341 gctggatctg atcggggcat ttaagaagcc ggtcaaccgg tcctccttgt acgcctttgc
    23401 cgctcaacag atcatcctgc aagatggcca ggcagagatt taccgcaggc tgatcgattc
    23461 agcccgctcg gagatgaacc ggatagacaa tcccgccgcc ttccagccca accggcacaa
    23521 cctggccata gccctcatgc tggatgatcc ggaacggaac gcaacagacg cgtaccagat
    23581 cataaaaaat tctccgggca agttggatgc cctgggccgt ttcggcacgg ccctcagccg
    23641 gaagggctta ttgtataaag cctggtcgga aatccccacc gatttttccg atgaagaccg
    23701 cagttggttt ttatacaaaa tcctttacgg gtataaccac aggataccca ccaaacccga
    23761 atggaaatgt tttgaagaca atatggtctt tgtcgacaga tattttcttc cttatatcaa
    23821 cgaaaatgac tgatcccgga gccttcatgc agcatcagac catctgtccc tacccgggat
    23881 taaggtcctt caccgaggaa gagagcctgt attttaaagg cagggatcag caaatcgacc
    23941 agatcagttc cctgcttgaa caaaagaaat ttttaatggt caccggggct tcaggggaag
    24001 gaaaatcctc cctgatctat gcgggcctgg tgcccaatgc ccgcgccggt ttttttaaag
    24061 ccaaatattc caactggatc gtggcggatt tcaggcccga acgaaacccg gtcaccaata
    24121 tggcccgggc gctggcggat aaatttgacc ttgctccggc aagtgtagaa acggagctga
    24181 gacggggata ctcctcgctg atcgatcttt acaccaattc ttcctattat gcggatgaag
    24241 gagatgaagc ctggaaaagc ctttccgcag aagaacaaaa acaaaaaaag cggcaatcgg
    24301 ccaacctgct catcctcctg gaccaattcg aagagttttt taccaatccg gagaattttg
    24361 ccaatgaggc cccttcccag gatgcccagg tggtggtcaa cctgatcctc gagaccgcca
    24421 ggctcgcctt aaaaaatgat ctgccggtat atattgtatg taccatgcga tccgattaca
    24481 tcggtcagtg ttcggccttc cggggcttgc cggaatacat cggtttttcc cagttttttg
    24541 ttccccggct gaaacgcaga gacctgaagt tagtgattga ggaacctgcc atgctcagtg
    24601 gcaaccggat cacccagcga ttggtcgaac gcctggttta cgacctggcc gaaggcatcg
    24661 atcagcttcc catcctgcaa catgccctga gccaggtctg gctggcggcc gatcatgggc
    24721 gggaagaaat ggacctgctg cactatgccc tggtcggcgg catgccttcc tccgatttac
    24781 cggacgaaga ccaggaacgt ttcaatacct ggatgaaggg cctgcccgaa tataaaaggg
    24841 cttatttcgc tgaaacgggc cttcagcgga tcattgagat ccatgccaat accctttatg
    24901 aaggtgcctg ggaatattac agccggcaga atcccgataa accgatcagc aggcaggaca
    24961 gcaaaaggat catcgccctc gcttttgcct gtctcaccaa aatagaccac agccgggccg
    25021 tccgaaaccg gatgtccctg gcggagatca cctccatcat caacctgccc catcttgatt
    25081 cccggaccgt aggccaggtc ctgaatattt accgggaaga agaaaatgca tttatccggc
    25141 cctataaatc gggcgagccg gagacccagc aactggaacc caactcggtc ctggacatca
    25201 cccatgaaag cctgatccgc aactggaaca aactgatcca atgggctaat aaggaattcg
    25261 actattattc cacctggcag gactttaaaa agcaaatggc ccgttggact gaaagcggga
    25321 aggaacccgg gtaccttctt cccatcggtc cgctgactta ttttgaaaac tggtataata
    25381 actgtaaacc gaatatatcc tggatccggc gctatgaaga agcaggtgat ccggccctga
    25441 ctgaacaaca atccgttcaa ttattgggaa atacccggga atttctcaaa aaaagtgcct
    25501 cgaaagtccg tttcacgcgg gcctttatca aatacggccc taaacggatc gccaccgtcc
    25561 tggctatctt gtttatgacc gtgctcagtg gattttactg gtacgatgct tcccaaaaac
    25621 aaaattcccg ggtgattgat cgcctgcagc agcaagcgtc cgatttggtt cggagtgagg
    25681 aggtcgccca aattgtaaag gccttatatc tgatcaccaa tgaacgatac cgtgcgggaa
    25741 gcctgatgga gcatttgaga tcgctccagc cgaaggatgc cctcaataac gggatcgagg
    25801 tttacaagca actgatcaag ctggatatga agcgggattt tgggctcaag accggtttga
    25861 ccgggttcat cgattccaca taccgtcagt tggcctccac tgaacccgac gccttttacc
    25921 tgatcaccaa actcaatgaa tataccctgc ttctgaccta tgaccagtat taccgtcctg
    25981 gcccggtgct tggggaaagg ctgaaacagg ccagttttta cggttataaa ctggtggaaa
    26041 aattcctgga tcatccggaa atggccagtc cgaccgccgg ggtggaactc aactgcagtc
    26101 tccagaactg gctcaccttc ggccaggcct ccgcggtgga aatccaatcc ctcttgaagc
    26161 gtttttctcc tttcgagcag gatagcgtca atccggtgtt taatgcctat taccggaaag
    26221 ggacctacga aaccaacgga cgcatcccca acgattataa cgggggatat cataccctgg
    26281 cttccctgta tgcggccctg ggacgctcag accaggtcat ccgttgtttt gatttcctcc
    26341 gcgggaccgg tcaaaacgat tatttcactg gaagcctgtt caataattac acccatatcc
    26401 tgggaatttt ccaccaattt ggttaccggg agcagtcggg ggacatgatc cgttggcttg
    26461 gccggtatta tcccaccaat accccgctga ccatacaccg taatgcagtg attcgggcag
    26521 gatatctcag ccatctgtac cgggtcaatg tggaccgggc cgtactgcaa tcctaccgcg
    26581 gatatttttt ccccaatctc tgcctggccc ccccaggtgt ttttgaatcc ctgaaccggg
    26641 actatgaagc tttgatccag caggtcccgg atcccaatga acgcaattat ttatttgccc
    26701 tgaatttcaa acggattgcg atgttcggat ccaaactggc cttcgatagg ggtttgccgg
    26761 cggatacggc ccggcttcat ggctggctgg ctaaatccgt cgctcatttc cgtgccatcc
    26821 cgaaggaata cctgcagcag accgtatctt ccactttacc ctatttcgca gacggggtgc
    26881 gcacccgcca agtcagccgc aaagacctgt tgatctatcc cgattaccgg gatggctggt
    26941 ttagctggac ttaccatact gacctgtttt ttaactatct gtcccgtgaa aatctgttga
    27001 tggagtatta tcccaccgcc ggcgatctgg aagcgctcaa tttctggctg gctaaagcct
    27061 atgaaataaa acccggtaat ttcagggagg cctatgacca caattacccg ctccctgatg
    27121 aagtgctgac caccctggat caattcattg accggcaccc tgagggcgga cgtttcgacc
    27181 gcaatctgct aaccctgatc ctggccaacc gtgcctttga aaaaaaggac agcagccagg
    27241 ccatgaagta tttcctcaga atcgatcagt cagccctgac ccggtcttcg aaccggtatg
    27301 aatacctcga aaaaacgttc ttttccaacc aggtcaagga cctggctgtc caccttgccg
    27361 atgcaggacg cctgatggaa gcggcgcgcc tggcggaatg gttcgtaagc gacaacgata
    27421 aagccctggt ctatatgcac ctgacgcacc agctttacaa caaaggggat ccttattctt
    27481 ttacctacct ggattccgtt ttttccaaga tggagaaagt cgattttacg cttttcaacg
    27541 gcccggtcct ggatgtccgt taccggctta cgaggctgct gggccagatc ggcagcgacc
    27601 ggctcaatca acgcaaccgg gaaatgatgc ggcaacttgc cgaaggccca aagaccctgg
    27661 ccctgaacca catgatgctc ggcgtttccc aggaaggcaa ttattacagg gccatcacct
    27721 ccattcccga ccggctgacc gaagcggaag agctggcttg cctgacgctc attctcaatc
    27781 aggccgccgc gaaacaggag agcccggatc aaaggcaggc atgggcttct ctcgaccatt
    27841 ggttcaatta tttttactat tacatatttt tcgtacccaa ctgatgagcc ggtatctctc
    27901 ttattattat ctgttgtttt ttgcgttgat caccggcgcc tttgcttcca tggcacaaaa
    27961 tgcctatggt atgaccatcc tgggtatcgc tgcgatctgt ttcggcctcc tctttgctct
    28021 tcaatggctt catacctgga accaaaagga caggaatggg ctggccctta ccgaactgac
    28081 ctgcctggtc ttgctctccg tcttcttggc tttacgggta ttcaatatcc attttcaatc
    28141 ggtcgaaatc ctgtttacgg gtacggccct gctcctggct atggtatacg gcatacgcct
    28201 gttccggatc atccaacccg ataaacgcct ggctggaaga atgacctggc tggtattgtg
    28261 ttattatctg agcctgattt tttttcttct ggccgtatcc ctggccccgc tgatacctgg
    28321 tgcggtgtcg ccttccggca tcctggcttt tattttactt ttcctatttg tcaccggcag
    28381 ctggatctcc cggaaccaaa tccaggaggg tgaaaaaatt tccggtttcc aggttgtcgg
    28441 ccgtttcaag gaccggtccg cccttctgct ctccctgttt gtattgtttg ccctctatac
    28501 cgggttgacc cggatcgggt ggttgccgaa aatgtatacc agcgtgtatc cccagactta
    28561 ttatcagttg ctcaacgagg ccgaatcggg caaggatatt cctgtaaatg ggaaataccg
    28621 tcatgaagcc ttcaaggaag aatatgaccg gttttcggag aaacatttga agaataaaga
    28681 ataattcgac cgcccctgaa ttctaactaa ctggctaact ggtaaactgc ttaactggta
    28741 aactacttaa ctgactacct ttgcgccatg atccttaccc tcctactctt aatcatcggt
    28801 tttgtcctcc tgatcaaagg cgctcagtac ctggtgggcg gagcctcctc catcgcaaga
    28861 cagaaaggga tttcaaacct ggtgatcgga ttgaccgtgg tagcttttgg aacttccatg
    28921 ccagaaatga cggtgagcct ggtcgcggcc ctcgagggaa gaaacgatgc gtcgtttggc
    28981 aacgtgatcg ggagcaacaa ttttaatctg tttttcatcc tgggcctgac cggtctgata
    29041 tatcccctgg ccgttcaccg caatacggtc agcaaggaga ttcccatgtc cctgggactg
    29101 gccctcctgc tcatcgtcct tgtgaatgac accagtctgg gcctgagccg aaccggtttc
    29161 gatatcctgt cccgctttga tgcgctgatt ctcctgctgg ctttcggtgg ttttttatat
    29221 tatgtctata tcaacacggt gaaaagcccg gaaacggagg tcacagtaga agtcaggccc
    29281 tattccgtgc ccaaatccgt gggattcatt gtgctgggtt tggccggcct gatcggtgga
    29341 ggtaaactgg tggtggacag cgccgtgacc attgcccaat ccctgggttt gagtgaaagg
    29401 atcatcggcc ttaccatcct ggccattggc acctccttgc ccgagctggt gacttcggcg
    29461 gtagcggcct ggcataaaca ggatgacatt gccatcggta acgtcgtggg atcaaacatc
    29521 tttaatatca ccctgatcct tggactgacc ggcatggtcc acccgatacc ctttaatccg
    29581 gccatcaacc gggatatcct gttgttgatt tgcgggacgg tcgcgctgat ctttttttta
    29641 ttcatcttca cccggcataa gctggaaaga tggaaagcct ttttgatgct ggctacttat
    29701 atcgggtata cggtatacct ggtgcaggaa ggataaaaaa tacgtttgtc atccttatgc
    29761 ccggcccgta gggtgcccac cgcactgttt acggtttact gataactgat cactaattca
    29821 ccacacccag atatttatca aaccacccca gggtttcttt taccagctcc tggcggggaa
    29881 caagatggcc tccttcgtaa atgatgattt ttttatcatt cgccggtgac ccgatgagat
    29941 taaacatcgg cttttgggaa gtttccacgg ggaaaaaagt gtcgtttttc ccgttcagca
    30001 tgagaaccgg aatcttcacg cgtggaaaaa aatggagggg atcaacttca gggaatgttt
    30061 tttgcatcat caggccgccc acatggaaaa cagccgcctt gaaccgggtt tccgcggcac
    30121 acattatgcc acccatggcg ctgccccaac tgtatccata atagccgatt ttttggatat
    30181 ccaaatcttc ccttgtttca aggtagtcca gggatcggcc aatatcctga aaccaggata
    30241 tgacgtggtc cctgtaaaat ttggtttccg cctgtaaatc ggagctcaac tggtcgccgc
    30301 gttcaaaggt ccctttgaat accggataca aaaccgccct accgcttttg agaataaaat
    30361 caaaggcgcg ggaggaggaa ttgccggaac ttgtcgaaaa ttttcgttca aagatgaccc
    30421 ctgagcctgg aaaaaaaacc acggtctgat aaggcggggt atggtttctc ggaaggaata
    30481 aatatgccgt gagacgttcg tgctgataag ctgcatccag ttcaatcttt tcaattttcc
    30541 atgcgccgct atcctcgata ggactgacct cggggttcaa aggggtttta tcgtaggcgt
    30601 attgccgcaa caggacctga aaagtttttt cgtcgaccgg tttttcagta gaataatccc
    30661 tgaatgcaaa atcaacctgg ccggctaagg ttcccagggt ggtatcggaa ggaaaagttt
    30721 tcatgcatct gaaaccattg cccggtgagc ggtcccattc cggttggata tagccatcgt
    30781 tgtaagcata ggtggggtcg ttccaacctc ccccaagaat aaaccggtaa cccttcttat
    30841 tgcttgcgtt tgaacaccac tcccggacat tgcctgcaat atcatatacc ccccaataac
    30901 ttatgccttc caggcttccc gccgtcaccg tcccctggcc attaaaattg ctcttgggta
    30961 tgatgcccca ggtgttgaat gtattggcaa ccatactcca atgaaaaacc gtaggcaagg
    31021 tctttcctgc aaatcttgca taagccatgg cctcatacca ggaaacgccg gataccggat
    31081 gatcatcttt cccgtccgga taggtgcctg cctcccacga ggagggtcct ggccggcccg
    31141 ttttatccac aaacaaagtc acggcctttt caaaagggag ggggattccc gctgaatgga
    31201 cctgcggcca atacacactg tccctgtacc ctccggcatc cacaaactgt ttaaacgctt
    31261 tgttgctgac ctcgaattgg tcgataagat attcacctac ccacttcccg ccgtgctgct
    31321 cgagcccgac tatattcatt ccggcctcgg aagcaggaac ccggaccatc tgctcaggat
    31381 aggttcccag gctatccaaa gtaaggttgc gtattctggg attatagacc gtgatgaatc
    31441 ccggtttttc aattttgacc cgggtaaatc ctctcgggaa ccagatgttg gtgagcgggg
    31501 tcgtccccaa ggatttccac tctcctttta gatcgtcgta atccttccag aacacttccg
    31561 ccccggaagg cctggtttca aagctaaccg acgcggcaat ctgcggccag agcaggatta
    31621 gggaggaatc ctttggaatg taacggctgg ctgccttcgc aaggtcaaat gccctggttg
    31681 ggatgatgct gttctcttca accattttct ggatctgcgg gatccagtct ttccgggcgg
    31741 tttgtttctt cttccatgcc gggaggacca cagccccaat cccaccgaac agcagcaggg
    31801 gcaggagcca ctgaagcaaa aagtactttt tcctcttcaa ggatttgaac cgggtgtatt
    31861 ttattccttt tccttcgagt cgcttcttca ggggaacttt taatcccgga ttgcttaccg
    31921 catagatttc aaggggctct ttcacatttt tcagaatgta tttcccgagt gaaaccgtct
    31981 ggatatccgt ttgattcttg atgtcatcat agactttggc tgagataaaa atactccccg
    32041 ggagggccag cgattccagt ctcgaggcga tattgacccc gtcgccaaac acatcgtcct
    32101 cctcgactat gacatccgcc tggtgaatgc cgattcgcag cggcactgcg ggggactcca
    32161 gcatattata ttgtaccacc agggccgccc ggatggaatc aatggcgctt ttaaaactgc
    32221 acaaggctcc gtcgccggta tattttacga tccgcccccc ataaagttta acttcggtct
    32281 ccaggcattt tttaagtttt tcccggtatt tcatagccat ccgctcatcc tcctgcatga
    32341 tggcggtaaa acccataatg tctgcaaaca ggatagcggc aagctcgtgc gtttgtggca
    32401 tgtattgaat ggtcttaagg gatgaataaa ttatttgtgc atcatggcct ccgctccgga
    32461 ccagttgtca ggcactccct gatgctggta tttattcgcg atgttgataa aatatttcgc
    32521 cgcatgatct tccggatcca accggaggac ctgttggaat ccttccgcgg cgaggccaaa
    32581 atcacggtta agataagcgc tcatggcttc gttaaaataa tccagggtcc ggagcttcaa
    32641 ttgaagctcc tctgcgggat agccgttgat gcattccacg atattcagca cattgtattt
    32701 cccctttaac tgcacctttc ccaggtgccg gatatggtac cgctccgcct gttgaatctg
    32761 aagcaaggcg tccccgctca gcaacaaggg agaacggtaa tatttggtga ggctttcaat
    32821 cctggccgcc gtattgacgg tatccgaaat ggtcgcggca tccagccggt attcatcgcc
    32881 ggtaatcccc atgatgagcg gcccggtgtg caggccgata cccgctttta tggcctgcaa
    32941 accctcccgg ctccgctcca ggttgaagtc ctggatggcc tcctgcatct caatggatgc
    33001 ctctagcgca tcttccgggg catccggaaa caaggccatg atcgagtctc caaggtactg
    33061 gttgataaat ccccggtggg cacggatcac gggacccaga cgggcattaa aagcggatac
    33121 aaaccggaaa ttttcctccg gggtcatttt ttcggatagc gtggtaaaat cgcggatatc
    33181 ggtaaacagg accgtcacgt tttttccaac ctggtcccca agcatgacat cggtgagcga
    33241 gtccttgccc agcgcacgga tgaattcact cggaacaaat tttttagtga cttcatgaat
    33301 cctcaaaatc gcttcctccc tctcacggac ctcccggata tttttttcat acaacatcgc
    33361 gctttcaata gccgtcgagg attgcagggc cagggtcacc agcaatttca ggtgggccgc
    33421 ggaatattgt tccgcctcca tgccggccag gatgatggcc cccatgatcc ggtgtttggc
    33481 cttcatggcc gcgtagatca gggattggac gggggcttcc aggatgcctg aatcttttaa
    33541 tacgtcgaat tcatttacga tttcggattg cccgcccaag ccgattttca gaagcaggcc
    33601 aggctggctt cgcaattttt cctcattaaa aaggggaagg cccgagcggg cagggatgtg
    33661 gagctgtttg gtctcctcat tccagagtac caccactccg ctgtgggagg gaatggaatg
    33721 tatggcctgt tccagcgtca gtttggcaat gacttccggt tcgatggtct gactcaattt
    33781 ttcagaaaaa ttgtaaatga cgttcagttc ctggtagagg ctgaggacct cggcgcccag
    33841 tttcttcttt tcggcttctt tctgaagctg aagggacatg agttgcgcga tcaggcgggc
    33901 ttgcggccct cccttgaccc aacccagtat ttcatcattc aggtttaccg gaaaggactc
    33961 cgactggccg gctaccgcct cgccaaatat caactggtgg tgttgatctt ccacggccaa
    34021 agcgttccct tgcatttcct gccaggactg caaccaggcc gtgaaatcac ttcttttatt
    34081 cagtaaggat tttagagtaa gcggagacat aagttataaa gggatggccc ggttagattc
    34141 aggtgaatta gtttcaaacg actcatgttc agctcaaggc cagtacctgc cgggcttttt
    34201 caaggatcat ttccgggtcg aaaggtttgg tcatataaac gtccgcgcct acttcctgcc
    34261 ctttctgccg gtcaaattcc tgtcctttgg ccgtgagcaa tatgatgaat accccctcca
    34321 ggccaagttc ccgtttaacc ttatggcaaa cttccatacc attcattttg ggcatcatga
    34381 cgtccaggaa caccaggttc ggtttttcgg attgaatcag atgcagggct ttctccccat
    34441 tgtcggcggt gatgaattcg acgccttcgt cttccagctc ctccagggtc tgttcgatga
    34501 gcatccggat atgcgcttca tcatccacga ttaagatctt ctgagccatt ctttctattt
    34561 tttaattcat tcaatatttt tatcccttcg cctttcaccc ttcgccattc accatccacc
    34621 atccaccctt aatgatatac cagaaacaac acattctcca tccccttttc aaattgcagg
    34681 gcctgaatgg attcctggtt ccccgagaga agggagttga taatgatgat atccggttgg
    34741 tgtaccagtg ctttctccac cagttccagg acattggatt caaggacggt gtaccctttt
    34801 gttttcaata cttcggtcag ggtgcctatg gtcccgctgt cttcgtccac aatcatcact
    34861 ttcttttttg actttccctg ttccagcaaa ttcccgattt cattaaagag gataccggta
    34921 tcaatcggtt tgttgaggta acggtccacg ccgatgcgga acccgcgggc tttatcctga
    34981 accacggaca ggacgatgat ggggatatcc atggtctgag gatcgttttt gagcactgcc
    35041 gccacatcga atccattcat ctcaggcatc atgatgtcca ggaggatgag gtccggccgc
    35101 ccttccctga cctttgccag ggcctctttc ccgttgcggg cttcttcaat ctggtaacct
    35161 gcatcgccca gttcctgctg gagcagggac cggatggagt cgtcatcatc cacaatcagg
    35221 atatgggcgg ctggtttatc gacactcaag ggagacaggg ccacttgctc tttaagctgt
    35281 tttaccagct catccaggtg ctggggccgg ggtgttttgg tcttggttac gggcaatgag
    35341 aataaaaagg tgcttccttt gcccaattca ctttcgagcc agatgcgccc gccgtggtgt
    35401 tcgacgatct ctttgcaaat gggcaggccc aggccggtcc ctttcggttt atccgtaagg
    35461 gtgtccccca cctgcttgaa ttgttcgaaa acggccgcat ggtccttttc cgcaatccca
    35521 atccccgtat cggatacact gaccacgatc tcatctctgt cccggaaaac tttgcaggtt
    35581 actgtcccct ggggagtaaa tttcacggca ttggagatca ggttgaccat gacctggatc
    35641 agcttgtccc ggtctccggt gatttccggc aggtcggtac tgatttcttt aatcagccgg
    35701 atggactttt gatcgaatag agaggtcgtc gctgcaatag ccctttccgc cacttccggg
    35761 aggaatacgc tctcctggtt ccactccatt tttcccgctt cgatcttggc gagatccagc
    35821 acgtcgttga tcagactggt caggcgctgg ccttccgata ccacgacatt gagattttcg
    35881 gatacttgct ggatggcttt ttccgtcctg ggctcagaga tgtccgtggc gggaaagatt
    35941 ctttcttcca gccttttttt aatgatcttg gcaaaaccga gcacggaggt gaggggggtc
    36001 cttaattcat ggcttacggt agaaagaaaa gcgcttttgg cctcattggc tttttcggcc
    36061 agtttgctgg ctgcttctgc ttcggcctgg gcttctttta cctcttcaaa caaacttgca
    36121 ttgttcaggg cgactcctac cgaagcggca atggtagtca gcaagcggag gtcattttca
    36181 ttgaaccggt tttcctgctc ggtgctttgc acgctcagca ccccgataat acgttcgcct
    36241 acgggtatag gcactcccag gtaagatgcg gaagacaccc ccacgggctg gaccccaagt
    36301 tgggcggtcg tttcatccac atctttattg atcagcaaag gctggccatc ccggataatt
    36361 tttgaagtca gcccttcgcc cagtttgcgg gaaggcatgt cctccccgta ctggtaaggg
    36421 aaatctatca tattggattt gggatcccac aaggccaggt aaacaatatt tgctttaaac
    36481 aggtctctca actgatcacc taccatctgc accaggtcat ggacatccag ttgggaagcc
    36541 agggcccggc tgatattatt gaccgtggtc aattcggcgg ccctttgttt ggattccgcc
    36601 agcaggaggg tcgtttcatc aaaaaggcgg gcattttcaa gggctacgct catgctgttg
    36661 gtgatggtgg tcaacaagcg gaggtccgat tcactaaagg cgttttcgat ctccacattc
    36721 tgaaggctga tcgagccttt caccacttcc ccgacgatca tgggtacaaa gaccgcggat
    36781 ttgggcggct ttcccatgga tacgccggaa ccgccaaatt ttttagcggt ttccaggtaa
    36841 ttttcgttga tcaaaatggg cttgcggtgc tcaatcatgt accggttggc ccagatgagc
    36901 ggacggggtt cgctgtaatg ccttgctcct ttctcaatga catagcgcca atgttccatt
    36961 cctgccgcgt ggtcgaaggt ccggatcagc accgtctggg tattgaaaag gtcgcacagg
    37021 cgtttaccga ccagttcata gattgcgttg atatccattt cccggaccaa accgtcctgc
    37081 acgctgttga tgacggccag ttccgccgtc cgttgttcgg tttctttcag caggtgggtg
    37141 gtctcatcaa agaggcgggc attttccagg gccacgctca tgctgttggt gatggtggtc
    37201 agcagccgga ggtccgattc gctgaaagca tgttcgcggt ccacattctg aagactgatc
    37261 gaacctttta cgagatcccc caccagcatg ggtacaaaga ccgccgactt cggagggctt
    37321 cctttggtga cgccggttcc cccgtatttt tttgaggttt ccaggtaatt ttcatttatc
    37381 aggatgggct tccggtcatg gatgagatgc ttgctggccc agatataagg ttgggattcc
    37441 actgtaaggc gcaggccctt ttcgatagca tactggaaat gttcgatgcc cttatcgtgg
    37501 tcgaaggtcc ggatgaccag ggtttgggtg tccggaaaaa gttcacaaag ccggtctccc
    37561 accaggtcat agatgccctt catgtcgagt tctttggcca ggccctcctg gacgctgttg
    37621 atcaccgcca gttctgcggt acgctgttcc gtttctttca acaaacggtt ggtttcatca
    37681 aacagccttg cattctccag tgccacgctc atgctgttgg tcagggtggt cagcaacctc
    37741 aggtccgatt cgctgaaagc atgctcccgg tcgacatttt gcaggctgat ggaacctttc
    37801 accacctcac ccacgatcat cggtacaaag actgcggatt tgggcgcctg acctgcagtg
    37861 accccggtac ctccgtattt atgggcggtc tctacgtaat tttctttgat atcaacgggc
    37921 tttttggttt gaatgagcaa tttgttggcc cagttaaaag gacgggctgc cacttcaata
    37981 tgttgcccct tttcaacggc atactggaaa tgttccaatc cggtttcatg gtcgaaagta
    38041 cggatgacca gagtctgcgt atccgggaac aactggcgga gccggtcgcc caccaggtta
    38101 taaataccgt gtatatccag ttctctggcc agaccatcct gcacactgtt tatgacggcc
    38161 agttccgccg tccgttgttc ggtctctttg agcaatcggg tggtttcatc aaacagacgg
    38221 gcattttcca gcgccacgct catgctgttg gtcagggtag tcaaaagtct caaatcggat
    38281 tcgttaaagg catgttcccg gtccacattt tggagactga tcgatccttt gaccacctcc
    38341 cctaacatca tcggcacaaa aacggcggac ttgggcgctt gtcccgcggt caccccggta
    38401 cccccgtatt tttttgcggt ttcgacataa tgttcctgta tgtcaagagg tttttttgtc
    38461 tggatcagtt ggcggctgtt ccagttcagg ggcctgggat ccacgatttg ccggaccccc
    38521 ttttccttgg cataatgcca atgttcaagc cccttctcat gatcaaaggt ccggatgacc
    38581 agggtttgag aatcgggaaa taaagtacac aggcggtcgc cgaccagatc ataaattccc
    38641 tggatatcca gttccctcgc caggccatcc tgcacactgt tgatcaccgc cagttcatcc
    38701 acccgctgtt tcagttcggc ggtgcgctca ttcaccatga gttccagttc ccggtgtttc
    38761 tcctgcaatt gcctggttct ccatcggaca atggcatata ccagggcccc ggccagcagg
    38821 aggtatgcca aataagccca ccaggtggaa taccagggcg gtaaaacggt aaagggaaag
    38881 gtggcttcgc tgctttccgt gtcataaatg tttttcgcct ttaccctgaa ggtatagtca
    38941 ccggccgcaa gatttccgaa ttccctggaa ttctgcagat cccagtcact ccagtgttta
    39001 tcccggcctt ccaggatgct ggaaaaccgg gtttcatttt ccagtttaaa aaacggggcc
    39061 gcataatcaa accggatcgt gttccaacct ttgtcaaata aggcctttcc ggagggcggg
    39121 ccgaaaccgg cataatagac cgaatcctca ttcaaggtga tcccgcgaag aatgcaggaa
    39181 aacggatcct gaaccgattc atccaggttc ccatcgtagc ggatgatccc ctccctgccg
    39241 ctcatccagg caactacttc ccccttgctt cctgccgaaa gggcgatgtc ccagatggga
    39301 tagtttctct tcagttcctt aaacggggca ttgacgattt tatgctttcc tccggctgcg
    39361 ggaattgcga caaaaacgcc ccggccaaaa ttggcccaga tccgtccccg tggatcggtt
    39421 atgcctaaaa gcgtgtagtc ggcgttatat acaaattgtt tatttaggat cactcccgta
    39481 tcgcggatga accgcttcag gccttcatca aatacaaaaa ctgaatccgc ttcggcagca
    39541 aaatacacgc tgccttcata ctccaggatg gttatgtttt tgcggggcag gccttgcctg
    39601 gagtcaaaga cctccacggc attttcgttg agcagccatt taccgctgct ccggtcgggc
    39661 ttgaccagtt ttacttcccc gataccctca ccactcagcc ataaattacc atcaggcgtt
    39721 tcgctgatca ggccgatgcc gagatttaaa atgggtgcga aggactccac cgcatatcct
    39781 ccggtcaaag gcgaatatct tacaatagcc agtccgctcc gcaaggcaca gattaacact
    39841 gtggtatcga gcttcgatct gagtatggac ctgaggtgga aatcataatt aataccgggt
    39901 atgacggcaa tgcccttgtt tccttccaat ttaatcaaac ccctctccgc gccgctgacc
    39961 agcagatctt ttccatttac taaaaaattt cccgtctggc cgctggaacc ctcaaccttt
    40021 tcaaaacgat tggttaccgg atttaactta taaataccgt tgttggtgcc cgcatagatt
    40081 tgatcctgat gggtccccac actgaaaacg gttttggcgg gcaaacccag cgcttcgttg
    40141 taaaaagtca gggaggacct aaggtcaaat ttggcgattc cattaaacaa gggcatccat
    40201 aggatacccc ggctgtcttc aaatacctgg tccaccgaat tgtcctgcag ccccaccgat
    40261 ttgtcgatcc gctggatgag tttaccctgt ttgtcaatga tcaccatacc gtcgttgaaa
    40321 gtattcaggg cgatcaggcc attgctcagg atacggcctc catacaggct ggtatttaca
    40381 ataaagggat ccgcttccgt tttgaaaggg gtgaagtcct taccatcgta gataaacata
    40441 ccttgcgtgc gggacccaat aagcagacgc tggtcatcgt aaggcaacat gacataaatc
    40501 cgttcattgg caaatttttc accgttcggc accaaatgaa aactgtcctg tcgcaattca
    40561 gtcaggccgc gggtccagat cctcacaaaa taccggccct tgacttcggc ccccacatgg
    40621 aagccctcct tgctttcgag aacccggaaa gcgctcccgt tccaaacaaa aatcttgtta
    40681 ttggtacgga aatagacttc atccctgaat atctgagttt cccagacttc ggcaaattgc
    40741 cggtgtggtc cgggcaattt ttctttaagc gatataaatt caagctgacc tttctttccc
    40801 cgggccaggt atcccagatc cccattggtt cccacgtaaa tggttccatc cttcgatttg
    40861 accaaactcc tggtctccgg cgcccccgga ggcttcacca ggtcccaatg ttggccgtcg
    40921 tagatcagta tcccaagggc atttgcaaaa tacatcaccc cctggtcatc ttcctcagcc
    40981 caccaattga cgccatcggc attataatcc tggtagcggt aattggtaat gaaaggcagc
    41041 cccttcacgg aatgttcctg ggaaaaaacc cgggaaaata ttccggtcca acaaaaggca
    41101 accgggaaca ataaaaacaa aaaccgagtg gagcaaggtc tttttagctt catatcatat
    41161 ttgttaccaa gactttacgg tcaaaatgct gaggaagaat ttttgaagcg acctcattaa
    41221 acaatgatat aaaatcctta cttaaatgta gcaatatttt ccagagaaac cgaaccgggt
    41281 ataaattgca ggatggatcc cggatttatg ggaaatgccg gcatggaaat tcagtccaag
    41341 agcgatgaaa aaccgatcag atatttcgtc gcattggtct gcccttcgcc gcttcaggac
    41401 agaattaacc ggctaaaatc ccgggtgaag gaagaatacc attgcagggt gcccatgaat
    41461 tccccccctc accttaccct gatccccccc ttttggattg aaccacgcaa tgaaccgctg
    41521 gtcttccatg ccctcgatgt tttccaatac ccttccggca ttccgcagat ccgcctccag
    41581 aacttctccc attttgacgg ccgggttctc tatatccggg tggtcgaaga cccttccctc
    41641 ctggaattaa aaacaaaact ggaatcacat tttatttcca tcctgggcgg ggcggtggtt
    41701 cccgatgtcc gggtgtacca tccccacatt accctcgccc aggccggccg gcgtaggtcg
    41761 gcctttcacc gggcccttga atactttgcc accggcgaat tcgaagatca attccatgcg
    41821 gataaaattt cccttttaag acacgatggg gtcgcctggc aggtgatcgc ggaaaaaaaa
    41881 atcgaatgag tttattgagg atacctgtca aaaccgtatt gccggtatat gttttcaaat
    41941 tgcgggtgtt ttctgaaagg atcgaaaacc cggtcaattt ttaaagtcct caggggtgtt
    42001 tccagtcgct cgagcgaccg gtttaaccaa taaaaacagg aatccggtac atttctggaa
    42061 gcgtaaactg tggctaagga aaaaccggcg tcttcggccc cctccgccgc taaggttttt
    42121 aactgcgcca ggatggcttc actttccctg aaccgacggg ttttgaactg tgctatacaa
    42181 agtttggcca gaaattcggg ggtcaggctt tttttatgaa tgaggatgga atcacagagg
    42241 aggatgaccc ttgtataatc cccggaaaga tcggcattat tcacttcaaa aaaagccagg
    42301 ttctgccggt ccgggaaaag tccatttcct tctcttatca attgggcggc cttgtccagg
    42361 cgaccggttg cctggtttac ccggattaaa atgaaataac tgttgtaatc caccggatcc
    42421 agctccatgg cctttgaagc gagggcggaa gctttatcaa aatcagccat ccagagtaaa
    42481 aagtatccgt agcggttcaa aatcaaggca tcattggggt tgagttgaag ggatttttca
    42541 aagaactccc tggctgccgg aaagtcccgt tcgatggacc atgccaacag ggccagggcg
    42601 gaatatcctt cggcaagccc gggaaaaagg gcaatggact tataagcata attttgggca
    42661 tcggccaggg cagccttgtt ttgaaagtcc cgctgtttat tgatgatcca ggccttggcc
    42721 agcgcgatgt acgcgtaaga tgcggcatag tttgaatcca actgcaatgc ctcctggttg
    42781 tacgcgatgc tttgttcgta ttgctgggca ttataagcat ggaggccttt caggtaaagg
    42841 gtataggcct ccgggctggt gacctggttc gcaggaaggt tattccctgg aagatttaaa
    42901 gtcaacttta agcgcccggc aatatgggcg gctatttcat cctggatttt aaaaatatcg
    42961 gtcatctccc ggtcaaaacg ctgggaccac aaatggctat gatcgctggt ccggatcaac
    43021 tgggccgtaa tccggatccg gtccccggat ttctgaacgc tgccctccag gatggtcccc
    43081 gcattcaatc tttttccgat ttccctcagg tccatttgtt cccctttgaa ttgaaatgac
    43141 gaggtccttc caatcaccct cagattatta attcctgaga ggaccgtgat gatttcttcc
    43201 gcgagcccgt cacccaggta ggcctggtcc tggtccgggc tcatgtccgt gaagggaagg
    43261 accgcaatgg aaggatcaac ggctgcccct ggatccgagg aatgggctga ctgatcgcgt
    43321 gtatttttta aattgaaata aatcaaagca aaccctgcgg caagggccgg caacaggtag
    43381 atcaaaacct ttcttcccga ccacgggtat tttttttgtt tactgccctg aggggaattc
    43441 ctgaccaccg ggctttttat gggatccccc tccaacaaaa tccggtagac tttgacgggt
    43501 tcccgtacat ttttaagttc cacggttttt acaaactccg accggatgtc ccttttattg
    43561 accaaatttt ggtgcacgga ttcggaaacc cagatgcctc cgaccggcgc catggcctgg
    43621 atccgggcgg ctaggttgac gccatcaccg aatacatcct cgttttcaaa caccacatcg
    43681 gccaggtgaa tgccgatgcg cagttgatat agaccggctg cccggcaggc ttcctggatt
    43741 ttaatggcgg cgagggtcgc gttcctggca gattcgaaac tggccatgat cccgtccccg
    43801 agctctttaa tccaacggcc ctggtactcc ccgataattt cttgatggat ttccctgttt
    43861 tttttcaaaa attcaaaggc cttcctttca tcctgcccca tcaaagcggt gtaaccgacg
    43921 atatcggtaa acatgatggc cgccaggcgg tgggtttgtg acatggcttc agaaacggca
    43981 aataaattaa gccgttttct ccatcctgca aaataagccc cgccccgggg gggcagaatt
    44041 taaaagcttt ttaatcaaga ctgcaaaata acaggggttt cttcatcatc attcccaggt
    44101 gcttgactcc cctggttccg agatcctggc agagacagca gatcttatgt ttccaaaacg
    44161 aaacctcgga agcgtcctga cagcagacag ggaaattatg aaaacggtgg aattttgtga
    44221 aaatggagcc gaggatcctt tggacttatt ctcaggtctt gggcactgaa tatcccggaa
    44281 tttctcttct ctcgctcctc tcaaaatcgc agcaccaacc tttctattat ctcccccctt
    44341 tgaaataaac ccatccctgc tcttgtttta aggcaagttc aatgacggca gatggcacta
    44401 ccaccgcttc tttgaggaga tctgttttgg tgaccattct cgaattcatc gaatggttac
    44461 aagctgcgaa tacgactccc ttttttgtca gttccgcaac tgcgtctttc gcttttgaat
    44521 aggccgtgat cagcaagtcg atccctccgc tgaggcagac cacttcaacc tccgcgccgg
    44581 gcagatcgtc cttgatgtgc gcagcctgga gggttacgcg ctgctgggag atggagtccg
    44641 cctcgttgaa ctggatcaca attttatgtt tattgttttc catcgactgg aaggtctgct
    44701 gtgcgttcat ccgcgccgaa aaaatgagca ggcagaacat aaattttaaa caagagattt
    44761 tcatcgtatt cgaagtttga ttttgcatga caaaaattca aagtgatggc tgtacgctca
    44821 catcgccagg accgcttata ggcttcattc atatgcgcct actctaccca cccaatctgc
    44881 atgaaaaacg gcccttctaa tttccatata atttataaat atttttttga tattattcac
    44941 tccgggcgcc agcctattct tcaggactac catccggtac gcattacccg ccaaccctat
    45001 tccttttctt atcctgcacg ataataaaaa gctaaagaat atgcttaaat tggatgatct
    45061 gtatttttta aacccaataa accatggatg aacttaaaat gatcaacaac ggcagcgggg
    45121 ccagcgtctc cggcgccgat ttggaaacgt tcaaatcaaa tttccggggc agactggtta
    45181 ttccaggcga tgccgactac gatgaagtcc ggaccctctg gaacggcatg catgataaaa
    45241 aaccgggcat catcgcacgc tgtaccggcg tggctgatgt gatggcttcg gtaaattttg
    45301 ccagagatca tcagatcctg ctggctgtaa ggggtggcgg acataatgtc ggcggcagcg
    45361 cttcctgtga cggaggcatc atgattgacc tctccctcat gaaaggggta tgggtggatc
    45421 ccaatacccg tacagccagg gctcagggtg gagcgacctg gggtgatgtt gaccgggaga
    45481 cccaggtttt tggtttagcc actcccggag gggtagtttc cactacgggg attgcaggcc
    45541 ttaccctggg agggggattg ggatggctgc gccgtaaaca cggtctgacc attgacaatc
    45601 tgatttctgt tgacatcgtc accgcagacg gccaatttca taccgccaac agcatgcaca
    45661 atgccgactt attctgggca gtgcggggag gcggtggaaa ttttggcatc gtcacttctt
    45721 ttgaatacca gttacatccg gtagggccca tggtgacctt atgcgccccc tggtatccgg
    45781 ccgaggaggc agatgccatt ttaccggtct ggaatgaatt catgaaaaca gccccggagg
    45841 aattgtcgtc cactgcaatg ttctggaccg tgccggctgc ccccggtttt ccggcggaag
    45901 cgcatggaaa gcgggcattg attattgcag ccgtccatag tggctccctg gaagatggcg
    45961 ccaggattat tcaacctctg agggaattag ggaaacccat gctggacctc agcggcccaa
    46021 tcccatggac ggcacttcaa tctgcttttg atggtttttt tccaaaaaag caacaattgt
    46081 attatttcaa atcccgcaat cttagacagc tcgatcaaca aaccattcag gccattattc
    46141 cccaggcctg caagcccccg gctccgatga tcctgatagc catctggaat tacggtggcg
    46201 ctatggcgcg tgtgggagaa caggaaacgg ccttcaccgg acgtcaggtt ccgatcttgt
    46261 tcggcgtgga tgcggtctgg gctgatcccg gacaaactga ggaagtattg gcctattcac
    46321 gcaacttttt atcatcgttg gagccttact caaccggtgg aatgtatgta aacttttccg
    46381 gccttggaga agaaggggaa actttagtca agtcggctta tggaaataac tatgaaagac
    46441 tggtctctgt taagaataaa tacgatccgc ataatttatt caggttaaat cagaatatta
    46501 agccaacggt gtagggtgca tatgggccta tggaaaaccg attcttttaa gcaggtcgga
    46561 aaatcggggg tcttccttaa gggatgccgg aacaaacgca ttgtatttta tttgaattaa
    46621 ggcaggatcg cgatccgcaa acgcctgttc caaatattca aatgccagat ccaaccggtt
    46681 caaatgcgcc gcggaaaccc cggtataaaa aggtaccacg tatccatgct tcttgctgag
    46741 caataactcc tcaaatacgg cctgcgcctg gatcagattg ccattcctgc aatgcgccca
    46801 aattaaacca caaagggcat gctggtgacg ctgggatatg gacaataaat gctcaaaaac
    46861 cgctatggct tcttccgtcc ggttaagttc aataaggcat aaacccacca ggcggtgaga
    46921 aaggaatgag ttgccgtcca attccactcc cattcgtgcc atagccagtc cttcttcgaa
    46981 ttttccacat ccatataaag tccaggcaaa atcagcgtga tcaacagcac ttagcggatc
    47041 cgcctttatt ccggcccggg ataaattttc cgcttcttca aactggccct tgatccaaca
    47101 caaatagccc ataccatata tggaaaaggc ctgggcgaat ttggggttga gttgcatagc
    47161 ctttaggtag gccttttgac ttttatccca atcccacaca aaagcggcgt aataattggc
    47221 cagggcaaaa aagccttcag caagctcccc atccagcttt atggcttttt cggcagattc
    47281 ttccattttc cggagtaccg ccgtacccgg tgcgtatcca tagaaagcca ccaaataatt
    47341 ggtccacgag taccctgaat gggctaatgc ataattaggg tcggccgcaa tggcttgttc
    47401 aaaatatttt acggcaaccg gaatggcgct gccccttcga ttcatataga atctgccttt
    47461 taaaaccaat tcataagctt ccgtgttctg ggtagcgtta ttaaccaggt tttctctttc
    47521 tttatccagc aaggtaatcc tgagctgttc tgttatagcc agtgcgattt catcctgaat
    47581 ggcgaaaatg tcctgaatgt cccgatcaaa tttttcggac cagagatgaa atccatcctg
    47641 tacactgatg agctgggcgg ttatgcggac ccggttgccc tgtttgcgaa tactgccttc
    47701 caggacatgg gccactccca atctgcggcc tatctccttc aggtcaacct gctttccctt
    47761 gaattgggcg gaggaggttc ttccggcaat tttcaggctt tttatatgca ccagggaatt
    47821 gatgagttct tccgcaattc catcgccgaa atactcctgt tccggatcat tgctcatatt
    47881 gacaaagggc aatacggcta tacttttttc aatcgtttgg gtccggtcct tcgccccgga
    47941 tgggtcactt ttactttctg ccccattcaa taccacttca tagatattca agggttcttt
    48001 tacattcttc aaaatttccg ttttaacgaa ccgcgtggtg atgtgatact ggcttgttaa
    48061 attgaatcgg accgcctccg aaatatagac accacccgga acagcaatag cctgaatcct
    48121 ggaggcaata ttgaccgcgt cgccgaatac atcccccgac tcaaacacta catcgccctg
    48181 gtggatccca atcctcagtt cgaattcccc ggctgcagca catttattct gaatgactat
    48241 cgaagcttgc actgcatccg ccccattggt aaaactggct aataccccat ctcccagttc
    48301 ctttatccag cggccacggt attcctcgat gaccggtttc tgcaaatccc tgcttttttg
    48361 taataatgag aatgcttttt tctcatccct gcccatcaag gcggtatatc cgaccatatc
    48421 ggtaaacatg atggcggcaa gttgacgggt ttgggacacg tgttatcttt ggaggtaaag
    48481 atagcgttaa gaaacctgtt tcagaaagtc aagcccgtca atcaaataca gacggcagat
    48541 aaccgccggc ttaattcatc accatacact tctcctgcaa tctgatctgc tccgacgtca
    48601 ggactacgga atagaccgcc gccgggagtt gatctgactg aaattcgatt ttatatcttc
    48661 cggcctccag cttcccccgg agcaaggtga acatccggtt gcctttaaaa tcgtacaggg
    48721 ccaggtggac attcccgtcc ccggcaagct ggaattcgac actggcttgg gagaccaccg
    48781 ggttgggata gattttgagg gcattgattt tttccgcatg ggtatgccgt tgcaattcat
    48841 agatgccgcc attttccccg gtgggaatcc ttccggggat ggctgttggg tttttggcga
    48901 atttgaaacg gcttgaagag aaacggggca gataccagaa tccaacggct gcttccgatt
    48961 gaatggtccg gctgatcccg gccaggcttt gcccgaccgt tacctgggtt ttataggtat
    49021 tgccggcaac gggctggcct ccattgctga tggccgtttt ggataacagg atttgagccg
    49081 gggccttcgc ccctacccat atgcagcagg ccagcaatag gagtaaatgg attttttggg
    49141 tcatggaact attggctttt ttgtgctttt tcgatatcct ttattatact ggccccatgg
    49201 agaatgggat tcgcatccat cttatccggg tacccggatt ccagttttgc cggatattca
    49261 acgcctggat taaacggctt gggatcatcc gtcttattcc ttaccacttt aaaatcttcg
    49321 tgtccgttgc gcacgcattt gacttcccag tcaaattcat aggttcccga tccttccatc
    49381 agttcctcca cctcaaagcc ggtggatttt ttactgacca cggccaggcc tttggatttt
    49441 ccactgagcg gggtgaggat gacggtcatg gtacccgtgt tggctacttt ttgataatga
    49501 tcgctgaatt caatggaagc cttgccattg accagttttc cggtgccccg gatataggcg
    49561 gccagttccg ggccttcgag gctgccgtac cagatttgtt tatcgggttt cccgggataa
    49621 tccattttga agtttttaat atcggcaaac aagacgccct ggttcgagtt atcggtgtac
    49681 atgccggctt tcaccgggct cccgacgata ttggaattac aaactccgat ataccccagg
    49741 tttgagccat tggtcgtgga catgaaggaa tgctcaattc cgttcggacc tttcgcgagg
    49801 aggattccgc ttttgatctc ggccctggga tgttggaatc ggtccaccaa aatcaaattc
    49861 accccgcttc cggtgctgta gtttgacaaa taagaaccgt ccgcatcggt tacggtcata
    49921 ttgcccccgc cgctcatact tactctttgc atgccgctga cgtcgtacac atttacggaa
    49981 ccacccaggt tgccgctgaa catggaagcc ctttcgctgc cagcgctgtt gcttgccctg
    50041 acgtatccat gaccactgga ggtggccaac aagccggcga tcacggcgcc cgaagaatta
    50101 taagtggcca ccgtcccgtg attatcggta gtgaccgaag cttccacgac atcccttccg
    50161 gaggcattgc ttacgctgac aatacctgaa cccccgttat tgatggttaa tctggctttg
    50221 tctgcgcctg ccgcgttggc tacgccaata taacccccat tgttgttatt gaaactcagg
    50281 taagacaccg cagccccgtt cggattgtca gtgaccacca ctccacctcc atttgacgaa
    50341 gtcatccggg cgacatcttt tccgcccgaa gtgttggcat gcagataacc tcccccgctg
    50401 gaattattgg tggtgatccc actcaggaaa gtgcctgccg aattgttgac aaataaataa
    50461 ccgcccccgc ttaccgtgtt attggtcatg ccggccaata cctttccggc attgttaaat
    50521 atatttacga ggccggtgcc ctgaacggtg ccggtgaggg tggctaattc atttcctccc
    50581 ggtccattga ccgatgcctt tccctggccg gcggtattca catttatcgt tgcagccact
    50641 tttccctggt cattgaaagt cgtgagatta ccccctccct ggtcgttggt ggtcaaccaa
    50701 accgcatccc cccctttatg atcctttgtg ccaaaaaatc ctccgcccaa tgtcgtacgc
    50761 gtgagtatgg acattaagcc accggtggcg ttattcacca cgatggctcc atgagactga
    50821 tcctgggtca gcctgatcat atcaaatccc ttatcgttcc tcagggccat gcctccgcct
    50881 ttaccttggt tggccgtgat gcggaccact tccagtttat caatattgtc cagtcctaaa
    50941 tagcccgtgc cctccagggt agtcatccgg ccgactttgt cgccggcggc tgttcccact
    51001 tcgatcaatc ctcctccgct gcttccattg ctcaatctcg cagtgtttcc gccattagca
    51061 ttatagatat tgaattcacc tccgaggttg gccgcggtga tcatttcagc acctattttt
    51121 tcggcattgt tccgtacatg aatatcccct ccccggctat tgtgggtcat gaacgctccg
    51181 cctttgcgct ccttgctggt tacccaggca aggccggcag tgccattata tgcgagggtg
    51241 cctacggcat tttcgttgtc atccatcaca tataccgcgc ctccattacc aaactgagcc
    51301 agatgggtct gtcttttacc gcttggtcca taggtcgaca caaaacctga gcctcccggg
    51361 aaagaaccca gatccgctgc cagggaggcc ataccgttga aaaacttagc gaagccggag
    51421 cctgaattat cccctccgat ttcgaccctg tttttagtga gatcctgttt gctgacgatt
    51481 tgccagaggc cttcaccgtt ttctccgacc ccaatgacat tcacaagttt ggtctgatta
    51541 ttaaataaag aaagttttcc gccattcagg ccgttcagca ataccccgcg gtgattggta
    51601 cctgacttgc catccgtgac cgtgacgctg cctccgtccc ttaaagcctc aaacacggcg
    51661 cccaggttgc ccatattgtt tttgagggag agggttccgt ccgcgaaagc attgtggccc
    51721 ataaaaaaag tattcaggcc gttgtaattg taaatattca aaaaaccgtt acccagggta
    51781 tcgccgccca tgtgaagacg gttcttattt acatccgatt tatcctggat gctgtaaaat
    51841 ccctccccgc ttttaccaaa ggtccccatg tagtggacgg gctggttggc ttcatttctt
    51901 aatgagaggc ttccaccata ccgggagccg gaaagggatg ccttgacttt tccggatatg
    51961 tcattaagcc caaggtcgcc gtcacccccg gccgcggata aatagaccgc ggttttggct
    52021 tcacgattga ataaataaag gtgaccttca tcgtcttcat tcccccccat ttcggccaca
    52081 ttctcagggt tattaaaaat gcttttgatt tcaaagagtc ctgcccggta tgcattaatc
    52141 cccagttggt gcaccaattg agaatccctg ttaaatattt tgaataatcc atcgccttcc
    52201 gtttcggtga gttgggcgga aacggaaccg gatctattga atacattgat caggcctccg
    52261 gcattgtcat ttgcggccag gaggagggtc ctgttctttt ccaccgaacc cagggtgatc
    52321 tgcccgtcgc tgaattcatt ggattgcata tcggccaccg gatttcccag ccggttattg
    52381 acccggatca ggccgtttct gtcttccagg gtcccgatct ggatcatttc cgtctcatcc
    52441 gtgttattga tgcccaggaa acctcccgtc acctcagtca ggcggacagc tctttttcca
    52501 tcctggctga ataattggat gagacctccc gcattgtcgt tggcgcccgc gatcacggtt
    52561 ggattcttca ataccgaatt cagggtcagc tgcccgtcgc tgaattcgtt ggcaaacaat
    52621 tcggccgagg aattcccgag acggttattg acattaattc tggcatttaa atcatcggtg
    52681 gtaatgccca tggtgatcac ctccgtcccg tcgttattca ggatgtcgat gaagccgccg
    52741 tttccattct cgaggatagc ggccatggtt tggccctccc ggttaaaaac attgatgatc
    52801 ccgccggcat tgtcggtggc tcccaagctc aaggtgggat tgttcaggac ggatcggagg
    52861 gtcagttcac catcgctgaa ttcattaacc ttcagctccg aaaccacatt tcccaagcgg
    52921 tttttcagaa taataaaagg ctgattatcc ataaaattgg aaaggcggat cgtctcattt
    52981 ccctcctcat taaagactcc cagcatgccc ccgccattgt cggtattgga ttgcatgccg
    53041 atcataagcc ggtcccggcc ggaacggatg aataatcctc cgtcgccgga attattggaa
    53101 ctggaactca cggcttcatt ccctgaacgg ttgtagatga ccaaccttcc acccaatgat
    53161 tccgaagtgg tcagccggag cacgggagtt tcttcttcat tatagagacg tagaattcct
    53221 gcaccttcgg cgatggattc caattcaagg gcagtgcggt tattgaccga attcaacttc
    53281 aaccggccat ctcccctcgc attcaccccc agttgcgagg ccaggatccc ggccttgttg
    53341 aagacatgga ccagtccacc ggcattgtca ttggagccca ggcgaaccgt ggtattggtg
    53401 agtacggatt ttaggttgag ttccccgtcg ctgaattcat tggccttgat atcaaccacc
    53461 tgcctcccca gccggctgtt caggctgagt aatggttgtc catccggaaa atgagtgact
    53521 ccgaaagttt gtgtgccctg gccattggac aaactcaaag tccctcccag attcgaagcg
    53581 ctggttgaaa acccggcaac cgccagtgaa ttttgattta ggaggtgcag ggatccggcc
    53641 ggtcccaata atatattcgg tttcaatcca atggtggtat tcggcccaaa cggattctga
    53701 atgctgatgc ccgttccctc tgacaattgg tttatcgtcc cagtgccgcc tccggaacca
    53761 gccgccgaga tggtaatggt tttggtcgcg tcattgggca ctatggatat gttattgcca
    53821 gctaccaggt tgatgttgcc tccgtcattg ctcactccgc tcaggctgga taaaacatta
    53881 gggacaattt tatcagcggt gatggtattg ttctgaattt cattattggt tatggcgttg
    53941 gggttaacgg aaatggtcgt cgtcggccca tccggaccgg tcaccgaaat ggcatttcca
    54001 ccattgatgc gggatatgcc ggcggtaccg gtccccggat ttacggccga gatggtgatg
    54061 gtattgtcct ggtcattgct gagaatggtg atgtttgcac ccgccacgat attgatattc
    54121 cccccatcat tcgtaacacc gttcagactg gaaatcaaag gggatgatat tttatcaatg
    54181 gtcacggcgt tgggttgaat tttttcagac gagatggcat tgttttccac atttttagcc
    54241 atcaggctgt aaggggtgga ggtcaattct atccgggggg tcatttcggg ctcctgcccg
    54301 gtgcgtatcc caagaaaata agttttgtca aaaggcagag tcaggggatt gattttcccc
    54361 aatatcgtgc tgaacacccc agcctgaacc tgaacatttt cctgcttttc ttcccacagg
    54421 aaaccggatt cctgggcaga agcatagatc cggaaggtca acaaatattg tccgtccagt
    54481 accggttgac cggaggcctg ggacaaaacc ccctgaatat tgatcgtccg gggagactgt
    54541 gcccggagcc agaaagggta caaatacaat agggcaagca gcgggaacat gaaagttttc
    54601 ataaatacag gataatttca tccaaggtaa gcgggcggaa tgggtctatc catcccctca
    54661 ggagggtatt ttgtgtcccg gattatgatt taatcatccc cctgcctgat tttcatcacc
    54721 ggatcccggc atcctgaaaa ttaattttag gatttacctt gctgtatggt attcccttct
    54781 ttgaattccg ttcgcttccg cctggaaaga ctggagcgca tgatggaccg gatcgcaatt
    54841 caaaaatcag ccctggttca aacggttcac ccggcacagc ggatgggagc gaccaacctc
    54901 cttcaatacc tgtccctccg caatgtggat atccgggatc tccaggatga tctgcatatt
    54961 ctggggcttt cttcactggc cagttctgaa agccatattc aaagacaatt gcaggccata
    55021 ctgcaaaggc ttgggaaaga gtacaaacgg ggttctctca atccctgcga ttaccggctg
    55081 agccggaagg agattttcag gcagagcaag ctgttattcg gcataaaaag ggatgaaaac
    55141 atccctcata tcatggtgac ctttgattcc agcttcgcgg acgattactt tttcatcaaa
    55201 caattgcttt tggatggtat gaacgttgcc cggatcaact gcgcccatga cgacgaaacc
    55261 tattggtcaa agatgatccg gaatttgcga aaagcctgcc ggaacaccgg aaaaccctgc
    55321 aaaatctata tggatttgcc cggacctaag atcaggaccc aattgttgaa aaaaggcagt
    55381 gcggacggca aagtcacggt aaaggaaggc cagttggtct ggctggccga cgacagccgt
    55441 ggatttaata aaaacgatat tgtgattggc acgggggagc cagggatcat accaggcctg
    55501 aaaacagggg accatgtaca catcgacgac ggcctgatcc gcggtaaaat cgtaaaaaaa
    55561 agcaaggctt atgcccaggt ccggatcagc cggatatcct cttcaaaaaa aaggattaaa
    55621 gcggaaaagg ggcttaattt tccggattcg agcctccgcc tgcccccgct taccgaatat
    55681 gacaagtcct gcctcccctt catcagcaaa catgccgacc tggtcggata ttcctttgtc
    55741 cgtcatccca aagacctggc cttactccag gatctgttga aaacgtataa aaaacagacc
    55801 ccgggcatag tgctcaagat tgaaaccccg gaggcggtaa aaaacctgcc ggccctcctt
    55861 tttcagggga tgagggaaaa atccttcggg gtgatgatcg cccgcgggga cctggcagtc
    55921 gagatcggct tcgagcgaat gagcgaaatc caggaggaaa tcctttggat ttgcgaagcc
    55981 ggacatgtcc cggtggtctg ggccacccag gtcctggaaa acctaaataa aacgggcctg
    56041 gccacccggt ccgagatcac ggacgcggcc cgggccgcca tggcggaatg tgtgatgatc
    56101 aataaaggca accataccct ggaggtgatc aaaacactgc aggacatttt cacgcgcatc
    56161 ggagggcacc acatcaaaaa aaggtacacc ttccgtcccc tgagcatcgc tacccatttt
    56221 atccggaacc ggtaagcagg atggtgatgc atgagcactt atcgcaaccc agaggccggc
    56281 cattttaaaa agtggtgcgc ggttacaggc gaaggctcaa attccgggat tttacaaccc
    56341 gttcacggct ttaaaatatg caataaccat tcactttttt cccgctgttt acattataag
    56401 ctaaaggccg atattggatt tgacttttaa tgcctggatc tccaccttca gagataaagt
    56461 tcagatcata tgcctgttaa aaatatatat cggttgtccc agcgtatgat tacctttaac
    56521 ctaattcccc ttacacgctt ctatacccaa cctgcactaa accatgcgta cctcaaaacc
    56581 atttaattta accgtatttc ttatccttat taagctatcc tttccggcgc tgcaaggcca
    56641 aacgctccag atttccgatt tcgccttata cggtaaaaat aaagtcctca tctcctcctc
    56701 caattccatc tctggaaacg gttcgattgg cagtctttca ctcatccagt cgaccggcaa
    56761 tatcgtgctt ggaggcggtg ttcacagtgg aggcacgatt gccctttcca atggcaatac
    56821 cgtttatgga gaaatcactg cggcaaattt ggcaaatttg gttacccccg tgcttactgc
    56881 agggtcaagc acctccttta aggggaacct ccgggtcaac ggaaacatgg ttatcgccgg
    56941 aggaatggtc gatggaaaca ttacccaacc taccggagca agctattccg gtcctgcccc
    57001 ggcaggtggc agatctcttc tgccatcgcc cataccgctt ccctcccttc ccacactccc
    57061 ggttattcct gtattcgtac cgggcaatac ggatattacc aacaatgcgg ttctgagccc
    57121 tgagaacgga atgggtaaat ggagaaacct ttctctttcc ggtaatagaa ccatcacctt
    57181 tgatggtccc gggatttatg ttttcagttc cataaacctg tcaaaaaaca atactttcaa
    57241 atttgatttc aagggacttc ccggtacatt tcaaattctg gtggccggag aaatgaacct
    57301 ggaaaaaatt gccgtcgaat atgccggtgt cccaaacaac gatgcttcag ccgccgcctc
    57361 ccggatatcc tcttttacca atggaaccgg cacggcattc agcatagcca atgggaacgg
    57421 cggatctaaa acccgctggc tgggtacggt atatgccgcc cgcggaaaca tcctgattgg
    57481 atcgggaaca ggcagtaccg aagtgatggg tgccctttgg agcggagctg aagtggtgct
    57541 caacagcgga gccaacttca gttttgtcaa gcctgcggac tgcatcccac caggcgccaa
    57601 tgccggtccg gaccaggtct tgagttgtac ggtttcctcc gtttcccttc aaggggcttc
    57661 ctcctctccc tttgcccaat ttcattggca ggcagagtcc ggaggcctta tcgcatccgg
    57721 cgccagtacg ccaactccgg ttattaccaa atccggtaaa tttatcttaa ccgtaacaga
    57781 tcccttcacc ggttgtgcct ccaaggatac ggcaacagtg gtctatgacc cctgtatcga
    57841 tcccggttat gagtccggga ccacgaaggt taatgacctg atcggttctg agctgaccgc
    57901 ccttttcgag cagggtgatt ttaacccgga gcaccaattg tttttactgg acagcaatca
    57961 ggaaaaggtg tttattgaaa tcattcccct ggccggccaa tacagccagg tgttaagcct
    58021 tttgcagact gctgaatatg gtctggatca ggtcatcccc aatcctcccg ggattctggt
    58081 gattaccggg cggtttccca tcatgaacct gaataagctc aacgacctgc ctcacctgat
    58141 ccagtacgtg cggccattgg tgtctccggt tgccggaaac gggattgcca atacggccgg
    58201 agataaagcc atgggtgcag atttcgcccg gaatggattt ggtcttgatg gttcgggcgt
    58261 aaaagtatgc gtactctcgg acagctataa tactttagac aaccgtgctg ccctggatgt
    58321 gcaaaatggc gatcttccgg gaccggctaa tcctgaaggc aatctcaatc cggtccaggt
    58381 cctgctggat tatccattcg gccgtaaact ggatgagggc cgggccatgc ttcagatcat
    58441 ccacgacctg gcccctaagg cctccctggc tttcaggacc ggattcctaa gcgctgggga
    58501 ttttgccaac ggggtaatcc agttaaaaaa ttcgggttgt accatcatta cggacgacct
    58561 cacctatgtc acggaacctt tcttcaccaa aggggcggta gcccaggcgg tggataacgt
    58621 aaatagccag ggagtttcct attttacctc cgcggggaat ttcggccgta aatcgtatca
    58681 agccatattc acccctggcc cgattcccga aggaaataac ctcagcggaa acgcccatga
    58741 tttcggggga gggcaggttt tgcagaaaat aaaattgaat aaagcgggtg cctatacctt
    58801 cgttttgcaa tggcaggatg aggtttattc attgggtcag tccgtaaccg gaacccagaa
    58861 tgacctggat atctatatta cggacgagga ggggagggtc ctctatggat ttaaccggaa
    58921 caactttggg ggagatccgt ttgaaatcct tcctttccag gtcaccgcag cgacagaggc
    58981 caatcttatg attgtacgcg cacagggtag cgggaatgta aattttaagt acatcatatt
    59041 caggggggat gccgagatcc tgaattattc aggcagttcg acgattttcg ggcatgccaa
    59101 tacgcagggg gccatgacgg tcggcgcagt tttgtattcc aacacccctg ctttcggcgt
    59161 agacccgcct accattgcct ccttctcatc cgttggcggc ggcctgatca acgggctaat
    59221 tccaaataaa cctgattttt cagcacccaa tggggtcaat accacggtat ccttcggttc
    59281 tcaggatatc gatggagacg gtttgcctaa ctttttcggc acttcagccg ctgcgccaca
    59341 tgctgcagct gttgcggccc tggcctatca ggcaagggta aaattcggat acggcctccc
    59401 cactccctca gatatccgcc aactttttat aaataccgcg agggatatgc atgctcccgg
    59461 gtttgattac agttccgggt atggctttat ccaggccgat gccgcaattt caagccttcc
    59521 ttcatcaccg aaacctgtga ttacttctct ggaatttcct gaagggacgg acctcctcaa
    59581 cggctccgaa ttacccatac ttcttacggt caacggggag taccttacgg aacaatcggt
    59641 catttatttc cgcgatcagg aactgaccac aatttatata gatggtaacc agctgacggc
    59701 gttcattcct ccgtttagcg gcaaccctgc cattaccgtt tatacccctc ccttatcgcc
    59761 cagcggacag gatggaggtt attccaattc catcaccttc ctggatatcc ccaaaaaaaa
    59821 cgttcaggtg attgcggaaa ataaatcgaa aaaatttggg gaaagactgc ctgagttcac
    59881 tgctggaatc tggattgatg ggatacccct gagcgaatcg ggactgaccc tgtaccaatt
    59941 gggccttgac caattgcgtt tttccacgtc agcgacggta atgagcccgg taaatcagta
    60001 tctgctccgt ccctatcgaa cattcgatcc ggaggattca cgggatgccg gactgctgga
    60061 cctgtataac tatgaatttg taaacgggat tttaagcatc cggagcatgc ctctaaaaat
    60121 cgtacctaag aaccaaaccg tggtatatgg cgacctcctt cccggggtcg aatacgattt
    60181 catttttgac gattccaata tggatcacgg agataaagcg gccttcctgg aaggaatccg
    60241 gaatgaatac ctctccggtt ttaaatccaa tcactgggta ttattcaatt cctatcccga
    60301 gtccaatatt gtcctgcaag ccagtgacct ggaaaatatg ggccttctgg ctagtaaccg
    60361 gtctgtgtcg aatgccatac ccttcatcaa cggggtccct ttcctcaatg gattaccgga
    60421 aggcaatacc agcccggtga cctcgttcat aaatctgaat cctcaaagca ttctcgatta
    60481 tcaaatcaat tcatcagagg caatccttcg taacggaaca cccttcatta atggctcccc
    60541 cttattcaac ggaatccctt acctcaatta tgccgggatc ttcaatagca tccctttcct
    60601 gaacggggaa gctaccgtgc gttatgaaaa taccaatagc gtacccttta tcaattcggt
    60661 gcctttcatc aacggcaccc cctatgtcaa cggcacccct tatatcaacc ggagcggtta
    60721 tgaagagatc aacggtaccc cctatgtcaa tggagtccct tacctgaaca gcggctttga
    60781 actgatcaac ggcaccccat acctcaacgg cacccctttc ctcaatgccg ggtttgagtt
    60841 ggtgaatgga atcccttatg tcaatggggt cccgctcatc aacagcggtg agatttattt
    60901 tatcaacagc gtgcccttcg tgaacaacaa tggcttgtac ctgattaacg gcgtgcctta
    60961 cctcaacagt ggttttgaga tcatcaatgg taccccgtat gtcaacggca ccccctacct
    61021 caacagcgcc ttttcactca ttaacggcac gcctttcctg aacagcgtac ccttcatcaa
    61081 tagtttactg gagatcatca atggtacacc ttatgttaac ggagctcctt ttataaataa
    61141 cagcaatgga ttcaccctgc tcaacggcaa cccctatatc aacggcgttg cctttataaa
    61201 cagcggcatc caggtcctca acggggtccc ttatctcaac ggggtccctt ttctgaacgg
    61261 cagtctggag ctggtcaacg gggtacccta tttgaatggc tatccgttta ttaatagcag
    61321 ttccgatatc ctgatcaacg gaaccccatt tttaaaccag cattccgaac tggcaagcat
    61381 cattgatgaa gatgatcttt ccctgggtga tcaaagtttg atattgcatt ccgtcaatgg
    61441 ccttacgggc ctgaccgcgg gagatcattt catcataccc ggcggataca cccccggagg
    61501 attatacaat ggaaatctcg atatcacata tggtttaggt aacctttcga tttcaaaggc
    61561 cgtattgacc gtaaccgcca gggatacctt tatgacctat ggtgacccgg tgcccctttt
    61621 caccggatcc ctgtctggct tacgttatac ggaccaggta caggttaact atattatggt
    61681 cgacggaaat ggtcaaccgc tgatcaaccc cggcgccgga tcccatttta tccagcccca
    61741 ggtcatctta tccgagccgg tcaattatca ggttaaccta gtccagggcc aattcacggt
    61801 agccaaaagg aatttgacga ttacggcagt ggacacgacc cgggaatatg gactccaaaa
    61861 tccagtgttc acccttgctt attccggatt tgcttccggt gatggaattt cagcgatcac
    61921 cacgctaccg gcgatatcca ccacagcgtc catcctttcg gatgtgggcc cttatcccgt
    61981 caccctttcg ggtggatttt ccccgaatta taacctggtt tatagtccgg gcattcttac
    62041 catcaccaaa gcgccattaa ccgttacggc aaacgatgca ggcatcagtg aaggcgaacc
    62101 gctgcccgct tttacagcca cttatcaggg actccgtaac caagacgccc tgctgatcgg
    62161 gcccggaagt ctccaatttg cggtgtttga ttcaaatcaa aatccctata gcggtgccgc
    62221 aggcacttat atcattaccc catacaacct gaatttcagt aaagggacga attaccagat
    62281 tacttatttt aacggttttt tgtatgtaaa tcccacaaaa aacgtccgac atattcgtcc
    62341 tttcctggat tgtgttgagg atatgggggc ccaggctaca ggattgaggt acaaagccag
    62401 gttttattat atcaatgaca acgatataac attttatata cctgaaggcg aggataataa
    62461 gattacttcc gcgggagtct attccggcca tcctccggtt cttttgctgc ccggaaccca
    62521 ttatttcgaa atttattttg atggtaaaaa aatgacctgg agcgtccgga ctaaaaacag
    62581 cgataaaaag agcgcttcca gcagcgaggc cagttcaaca tccaacaaat gcgcttccag
    62641 ctccaatctt gccggccgaa atccttcacc cggactcgtt caagggtctc ctgccggagc
    62701 atggggctta atcgctattt tcccaaatcc ggtgcaatcg gatttcagta taatcagttc
    62761 aagggaagtc attcattcca ccggcctgga gatggttgac ctggcgggta gacgggtaaa
    62821 cgcctggcag gcgaatcaac tggatgaaca ttccatttta atcaaagccg gtcccttaaa
    62881 aaccggcatc tatttccttc gaattaaatt acaggatcga attgaaacca ttaaatggat
    62941 aaaattatga catagctcag tcagcggtgc atgccgtgta aaaaatgtgc atacagcatc
    63001 acccggatcc attgaagaaa agccctttat atttaatagc cgcatgttta aaaattgttt
    63061 tgttttttta ctcttcctat ccattctgcc ccggacttct ggacagatgg aattggtgga
    63121 cagcctgaaa ctccggattg agaccgccga ggaggattca gctaaaatga aactgtacct
    63181 ggacctgagc caggcttttt ataacaattc ctgggacagt gccatccact atgcccagct
    63241 ggcccggacg atcgcaacaa aactggaaca tcacagaggg actgccctgg catataagaa
    63301 catcgggttg gccaattatt tcaaaggtga gcccttattg actatagaag cctgggaaaa
    63361 ctccttactc tttttcgaaa aagctatgga caaacaaggt attgccaata ttttgagcaa
    63421 tctcggaggt gtatatgaac ggagtgatga agccagggcc ctcgattatt acctgaggtc
    63481 gctcatcctg gcagaagaga tcggcgacca tttccggatc gccaccctgt ccaacaatct
    63541 gggtgcttta tatgataaaa agcccgctac ggtggataaa gcacttgaaa attaccagca
    63601 tgccatagcg gtatctgaac aatacatgga taccgatccg cgctttgccg aagcaatggg
    63661 tattgctacc ggaaatatgg gggaaattta tctgaaacga gaccaggtcg attctgcgct
    63721 cttttatttc cggatcgggg aaaagtacct gcaaaataaa tccagcctgc ccctggcctt
    63781 gaataacata ggtaaagcct ttctaaagaa acttgatttt gccactgcct taaaatacca
    63841 gcaacaggcc tacgaagaag ctgcccgccc caataataag atgtatatga cctttgccct
    63901 gctgggcatg gcagatacct atgcccagca ggggttgact gccaaagcca ttgaactgta
    63961 taaaaaggct gaaagttatg ccctcgagct caatatcaat gatgaattgc gattggctta
    64021 tgcgggcctg gcttcccaat acagtaaaac cggggatttt gcaaaggcct accagtttca
    64081 gctaaaactg accagcatta acgaggtgat atacaacaat gaaaccgata ataagctggc
    64141 caacctcagg ctcgattttg accttaagaa aaaagagaat gaaatcagcc tgctgtccag
    64201 tgaaaagtcg ctgcaggacc tggcgctgaa ccggcaaaaa ctgattaaaa atgccctcct
    64261 tgccggactg gccctggttt ttctcatcat ctttatctta taccgcaatt accggcaaaa
    64321 aacagctgtc aataaattgc tggataagca gaaagccgaa attgaaaatc tggtcctgaa
    64381 catactgccg gaggaagttg ccctggaatt gcggcaaaac ggatctgcca ctccgcgcta
    64441 ttatgaaagc gtctcggtaa tgtttacaga tttcaagggt ttcaccaacc tgacagacca
    64501 gatgtctccc caggagatcg tctcggaatt aaatacttgt tttatggcgt ttgataacct
    64561 gatcgaaaaa tatcacctgg aaaagattaa aaccatcggg gattcctata tgtgcgccgg
    64621 gggcatacct acaccccaaa aagatcattg tatccgaatc atgcaggctg cgctggaaat
    64681 tcagcaatat atacaaaact ttaaccttca tcggggtaag tcagggctcc caccctggga
    64741 aatccgcatc ggaatacata tcgggcctgt cgtggctggg gtggtgggaa aaaagaagta
    64801 tgcctatgat atttggggca gcactgttaa tattgccagc cgcatggaaa gcagtgacga
    64861 acccggaaag atctgtattt cctccaatgc ttataacctg gtaaaagata aatttgagtg
    64921 tacctatcgg ggtaaaataa atgccaaaaa tatcggagac attgacatgt attacctggt
    64981 taaggaaata caccacgctt gaacccctca ccaagccccg acccaaccct aaacccgata
    65041 atattgcagt gggcaatgtg gtcggcaggg agggagaaca gggagttgtt tgttttaagg
    65101 tgatgcgtaa ataaattgat tatttcctcc gtgtgccccg tgtgctccgc gcgcatgcca
    65161 gccgggcgcc acaggcgccg gctgtattat gttcacacgg agggtgggag agcacggagt
    65221 tgtttgtttt aaggtgatgg gtaaataaaa tgattatttc ctccgtgtgc ccgcgcgcat
    65281 accagccggg cgccaccggc gccgaccgta ttatgttcac acggagggtg ggagagcacg
    65341 gagttgtttg ttttgaggtg atgggtaaat aaattgatta tttcctccgt gtgcccgcgc
    65401 gcataccagc cgggcgccac cggcgccgac cgtattatgt tcacacggag ggagggagag
    65461 cacggagttg cgccaccggc gccggccgtt tatgttcaca cggagggggg gggaaagcac
    65521 ggagttgttt aatttaaggg gatgggtaaa tataaatgat tgtttcctac gtgtgctccg
    65581 cgcgcatgcc agccgggcgc cacaggcgcc ggccatatta tattcacacg gagggaggga
    65641 gaacacggag ttgtttattt taagattggt aaataaaatg aatattttct ccgtgagctt
    65701 cgtgtgctcc gcgtgcgtac cagttgccgg gcgccagagg cgccggcctt gttagaccca
    65761 cacggaagga aggagatcac ggagatgatt tttgtaattc gagggcggtg ggtaaaatta
    65821 aaatgattat ttcctccgtg tgctccgcgc gcctcatttc tccgtgcgaa aagctccgcg
    65881 ccctcctttg ggtgaccgtt tccccggtcc tttaccagaa ttggcggctt ttagctaaaa
    65941 gcctgaaata tctatatttg cgccatgttt ttatccgtga tcctcctcct tttgggcttc
    66001 ggaatattgg tcaaaggggc cgatttcctg gtgaaaggcg cttcctccgt ggctaaaaga
    66061 aaaggcattt ccaacctcgc catcggcctg accgttgtgg ctttcgggac gtctatgccc
    66121 gaacttacag tgagcgtcct ggccgccctg gaaggcagaa atgacgcttc cttcggcaat
    66181 gtcattggca gcaacaattt taacctgctg tttattcttg gcgtagccgg cctgttatac
    66241 cccttggccg ttcaccgcaa taccgtgaat aaggagatcc ccatttccct gggcgcggct
    66301 tttttacttt ggttcctggt caatgattca tggaatggaa tgggcggctc cggcatcaac
    66361 ggcctatccc gcctggatgc cctgatctta ttgatttttt ttagcgcctt tatttattac
    66421 gtttacctga gcatgaagga agcttcggca aaaaatgcgg aggaaggata ccaccaatac
    66481 agtatgccgt tttcgatcat catgatcctg ggcggcctgg ccgcgctgat cggtggcggc
    66541 aaactggtcg tcgaccacgc cgtgaacatc gctcagtact ttggatggag tgaaaaactg
    66601 attggcctga ccatcctggc catcgggacc tccctgcctg aactggccac ttcagcggta
    66661 gcggcctatc acaaacacga tgacatcgcg atcggtaacg tcattggttc caatattttt
    66721 aatatattct tcatcctggg catcaccgga atgatccatc ccattgacta taatacggag
    66781 atgaaccgcg acatttatgt tttgattgcc gggaccctgg ccctgatggt atttcttttc
    66841 attctcaccc ggcacaaact ggaccgatgg aaggcattca tcatgcttgc ggcctatatc
    66901 ctttacacgg gatacctcat caaactggga taggtttatt cgcccggatt ctcttcctgc
    66961 atgggcatcc acaacttcgc cgcatccgtg gtctcggtga ggggagccgg ttgacgattg
    67021 aaccttactg ataattccgc ccggttccaa cggtaaaata aataaatgcc ggctacctgg
    67081 atcagccatg cgatccagga tccctcatat ccaaaatccc ctccgctgag tcccgggtat
    67141 ccggtatcct ggaattgaat gaaactgtac acatcgatgc cgctcacatt aaaatccagc
    67201 aggctggcct ggacaaaatt ccagcccgca tgcaggccgg tgcagaccca gatatttcgg
    67261 taacggataa aaaaaagggc catcagggtt ccgccggcta aaatattgag gaacccgatc
    67321 caggtaaaat gttcgttgcc caggtgcatc agggcgaaaa tggaagcgga caccacgagg
    67381 gcggccaggg tattgaagcg cgcttcaatc atcgggatga gataagccct ggtcatcacc
    67441 tcttcccccg cgctttgaat gataaaaaat aaaataaaac cggcaaaata atatccgttc
    67501 cagtctgccg gcagcaaatg aatttgtcca agaaaccaaa ggatgagaaa gccgggcaaa
    67561 accagggcaa agctccacaa ccatccctgt ccaaaataaa agaaggaccg ctcgtaccca
    67621 aaacccagtc ccgatagcgg acgcttaaaa atagagtgat gcgcaatccc tgcagccagc
    67681 gtgccactaa gaagcaccgg gataaaagtg ctggtcagca gggtataggg gctggtttgt
    67741 tcattcagat cggcggaggt ccaaaacagg tattcgttgc ccgaaatcca ttggatgccg
    67801 gtgacgatca aagccccgga ggccacaaac agtgcggcaa aaacaagcaa ttcaaccagg
    67861 acccatgcca atcctttaaa tagaactttc atcaggattt ggttttttct tcaaataagg
    67921 cttcaaattc cgggctttcc gttttcatcc aatggtccca gaaagtaaaa taaagaccgt
    67981 aattaaaatg gaagcggctg tggtgcaggg catggtgggt ggcgccgatc caccatttcc
    68041 caagccaatg acggtgggtc cccgaaggat aaatctcaac attgaggtga ttgaccacac
    68101 tggtcaccga catgatcaaa aacagggcga tcagcgtgaa cgggtgaaga ggcattataa
    68161 agatcaggat aaaaaccggc agggcctgca gcacgctctc caccgggtga aatgaaaagg
    68221 aagtccaggc cgtggtggtc aggctttcgt gatgggtacg atgcaccaat cggtatacgc
    68281 ccgggcgatg catccagcga tgcagccagt agtaatacgt ttcatggatc ataaggacca
    68341 ggaacaaact gggaagcaac caccaggcct tccacccgga caggtctgta tagatgaggg
    68401 taccgccccg ctgccagaaa taaatggcca gggtccccat tacagcaaaa agaagggaag
    68461 caataaagga ataccggacc tccatccgga cttgtttact cttggccggc ctcaggttta
    68521 ctttttggga atggtacctg ccggcaaacc ggacatagaa gaggtaataa aacacccctg
    68581 ccatcaggac atacctgaaa aagatcgcta tcgctaaagc catccaggta atgatcaggt
    68641 taatcaggtt tccctggctg agatccaaca tcgcctgcaa gttatagtgg aaataggaaa
    68701 atcccgaagg gcggcccaag gcgctggata ccctccccgt ctatcccatc aaaaaaacaa
    68761 aaatctccgc ctgtagattt tcctgagatc cttttcaaac aaggtataag ggtcctcgta
    68821 aaacttcagg aaatccggca cgctgggctg acccggaaaa ggcgcgtaat aatgggtagg
    68881 gctgcgcaca tcattcctcc aaaccagtac ataggacaac cggatcccgg gccggcgaat
    68941 ggttttcagc agggtggctg tccaccattc cgaattggga atggactcca ggcccgtttc
    69001 agtaaaagcg gccagctttt tattcgcttt ggcgattcgg gaaaggatgg tcaactgacc
    69061 ggcggccttt tcaatattgt aggtatccct gcccatgtcc cggtaattgt ccatgcccac
    69121 caggtccacc caggcattcc ccggatatcg ctccaggaat tcctcttccg tattgaattt
    69181 gcagtccgga gaaaatgcat agaggaggtt gtgcacctgc tgggaatcac gtaggtacct
    69241 tacagtgaag gtccacactt cgataaattc ttccctggtg caatggggtt ttccccacca
    69301 aaaccagccc ccgtccaatt catgaaatgg tctgaaaatc atgggtgcga gcctgccttt
    69361 ttcatttttt accgacttgc aaaacaaggc cacggtatcc aggatgcctt tatacttttc
    69421 atggtgagaa ccgcccggtt taatcaaaga catggccggg gcggaaatgg aatcccgcca
    69481 gtaaaatcca cccctggatg ccggattcat aaaatgccag gccacggtgg tcacccctcc
    69541 ccgggcaaag gtggccagca cattcttttt taaattttcc atcgtcctgc cgatggcctc
    69601 cgggggccag ccggacaatc ccatgaaatc aatgccgatg accgccgggt gggaaccggt
    69661 cacggatttg acatccgatc ggtcttcctc cccggaccat ccatggccgt attccgtggc
    69721 atgttgatgc ccgaacaaga ccttttttcg ggaaagcttt ttcaggtttt taaataaggc
    69781 cttggtctcc cttgtggctt tggggtcgat ctggccatag gccgctgact gaatgatgag
    69841 taaagcaaga agaagggtca gatatggttg cgcctgtttc ataaaaagga aagataaaca
    69901 agtctgcacc ttaaatagtc cttacctccc atttaatatt aatactattt tattgaataa
    69961 taacaggatc ccgcttcatc tacgatccgg tccccgaccg gaaaccaggc ttgatttaat
    70021 aagtaatgag aaatcgataa tttcaccagt atactttaaa ctctaattca aaatgatccc
    70081 caggtttcta tacttaacct taacctcaac cttaacctta accttaacct caacctcaaa
    70141 ggcccaaagc ccccaaaaat ggaaggtctc tgatccgccc ggcccttata aagaggtctc
    70201 cttcaccgtt cgggaaggca cctggatgaa tctggatctg gaccccgaag gaaaggaaat
    70261 tgtttttgac ctgctggggg acctctatac cctgcccgtt gaggggggca cagcccggtt
    70321 gatccggggc ggtcacgctt tcgaagttca gccgcgcttc agcccggatg ggaaaaaaat
    70381 aatctttacg tcggatgccg gaggagggga taattgctgg gtaatggacc gcgatggtca
    70441 aaatgccaaa tccatcacca aagaagattt tcgcctgttg aacaacgggg tctggactcc
    70501 ggatggagaa tttatcattg cccgaaaaca ttttacctcc acgcggtctt tgggggcagg
    70561 cgaactatgg atgtaccatg tgagcggagg caagggattg caactgaccc ccaggaaaaa
    70621 cgaccagcag gatctgaatg aaccctgggt atcgccggat ggacgatatg tgtattacag
    70681 tgaggacatg taccccggag gctttttcca atacaataaa gatcccaaca accagatctt
    70741 cgtgatccgg cgctacgacc gtgaaaaagg aaccagtgaa actgtgaccg gaggagccgg
    70801 tggagcgttt cgtccccagg tatcccgtga cgggaaatat ctagcctttg taaaaagggt
    70861 caggaccaaa tccgtgctgt atgtccgtga actggagacc gccagggaat ggcccgtatt
    70921 tgatcaattg tccaaagacc agcaggaatc ctggacgata ttcggaatct ataccggttt
    70981 tgcctggtcc ccggataatc gatccatctt tatctgggcc aatggtaaaa tcaataaagt
    71041 gaatgtggcc ggattcaacc agtccaccga ggtgcccttc acctgccagg tcagccagag
    71101 aatctatgag gccatccggc ttaaacaaaa cctcaacccc ggatctttcc aagtgaaagc
    71161 catacggcat ctcgtgaccg atccccaggg gaaatacatc gtattccatg ccgccggcca
    71221 tttatggaga aaggaattgc cggctggcct gccccaaagg ctgacccgga atggcccttt
    71281 cgaatttgaa cctgcttttt ccccggatgg gaaatacctg gtctatgtcg ccctgggtga
    71341 ttccgcctcc tcctcccttt ggaagctcaa taccaatgac ctccgttcgg aaaaaatatc
    71401 ggaagaacaa gggaattatc ggaccccttc cttttctccg gacggaaaac aaattgtgtt
    71461 tgtcagggaa gggtccagcg acctcatggg ccctgcccat acggccaggc caggcatata
    71521 tatcatggat gcggcaggcg ggccggcacg ctttgtcacg ccgcatggca gccaacctcg
    71581 gtttaatgcc cggggggacc ggatttatta ccagtccgga ggaggcatga attctacatt
    71641 aaacagttgc aaaccggacg gtagcgatga ccggacccaa atcaaaagca cttacgggag
    71701 ccagtttacc atttctccgg atgaaaaatg ggtggctttc atcgacctgc ataaagtata
    71761 tatcgcagcc ttgccacagt ccggaaaaat cattgacctg ggaagcggga ctgcggattt
    71821 cccggtacgg caggtagcca gggatgccgg cttcaatctg cactggagca gcgacagcag
    71881 gaggttgcac tacaccctgg gcaatgaata tttttccata cccctcgaag accgttttga
    71941 atttattgcc ggcaagccgg aatccgggtt taaaatgccg gaatccggaa ttgaaatcgg
    72001 gctgagcctg ccgatggaca aaccggaagg ccgaattgct tttaccaatg ccaggatcat
    72061 cacgatgaaa ggggacgaag tgatcgccaa tggaaccctg ctcgtggagg ggaatctcat
    72121 aaaagcgatt ggcaaaacgg gcacggtggc cattcctgcc gatacccgca tcatcccttg
    72181 ccagggaaaa accatcctcc cgggatttat tgatgcccat gcccacggca accattttaa
    72241 taccggtcta tctccacacc agttctggcc ttattacgtc aacctggctt atggggtcac
    72301 gaccatgcat gatccttctg ccaatacgga atgggtcttt gcccaaagcg aactccagaa
    72361 agccgggagg atcgtggggc ccagggtttt ttccacaggt accattttgt acggagcgga
    72421 tggcaatttt aaggcggtgg tcaataacct ggaagatgcc cggagcgcta tccgccggac
    72481 gaaggctttc ggtgcctttt ccgtaaaaag ttacaatcag ccccggaggg accaaaggca
    72541 gatgatcatc caggccgcgc gcgaaatgga tatcgaagtg gtgcccgagg gcggctcctt
    72601 tttttaccac aatacttcta tgatcctgga cggacacacc accattgaac acaatatgcc
    72661 ggtggccgaa ctgcacgagg atatcatcca gctttggaaa cattccaaaa cggcctatac
    72721 cccaaccctt atcgtatcgt atggctcggt cagcggggaa tattactggt accagcatac
    72781 caatgtctgg gagaagaagc ggttgttgca gtttacgcca aggtccatcg tggatacacg
    72841 aagccgccac cgcaccatgc tgccggagga agaatatgaa aacggtcaca ttctggtatc
    72901 caagcaactg aaaaaattga acgatgccgg agtaaagatc aacatcgggg cccatggcca
    72961 gttgcaagga ctcggcgccc attgggaaac ctggatgatg acccagggag gtatggacaa
    73021 tcaccaggct ttaaaaacag caaccattaa tcctgctcaa agtctcggcc tcgacgattg
    73081 gatcggatcg cttgaaccgg gaaaactggc cgacctcctg gtattggaca aaaatcccct
    73141 ggaaaacatt tataataccg agagtatccg ttacaccatg gtcaacggaa gattgtacga
    73201 tgccgaaacc atgaatgaaa ccggcaatta cgacaaacac cggaaaccct tttcctggga
    73261 gatggagaaa aatgccggct attttccgat aatcggggaa agccaggaag tcggaaaatg
    73321 tttatgcggg aaacattaac ggtggttagt gggtggtggg cggtttatca gttggtcagt
    73381 ttaccagtta gtttggagcg ctgattgctg attgctgact accgtatagg aaatttcacc
    73441 ccaaacccga tattatacag gttggctccg aaatcttcat catcaaactc atcggggata
    73501 tcattcacgg gatccaggat cttggaaaaa ctatactcca gtccgaattt aatgtttctg
    73561 ccgaaaagca cccccaatga ggtacccagg taattcagtc cgccttcgcc gaccacgccc
    73621 tggtaaacgt acctaggcgt caggtaccag gcggtccgtt cccccggatg ataggagagg
    73681 tgtaccggta ggtgggcctg aaaaagcact tcgtccagca cgattccctg tattcctagg
    73741 ccgggtccga ccgcaagggc aaatttggaa gtttgatctc cgaccaattg ctgttttaca
    73801 tcaatgactc cgcttaatcc ggtgctgatc ttgatgccca ggtccgtctt ttggccaagg
    73861 ccataccttc cccagaactc aagattgggc agggcgaagc tttcgtcggt accgaaactt
    73921 tccgaaatgc ccgcaatacc caggctgagc ccgatctccc cttcattctt tggggttgtt
    73981 ctcgcggttt gaaaagtgga tatctgggca caggaaccga gcaccagaat actaataaaa
    74041 agcagtccaa atcgaatcat cgcacaaagc taaaaacttt tccattctgg tttgggtgaa
    74101 gctttgcttc ggcctggtat ttatatacag acgataaaag gaaattttta tcgatccgat
    74161 tgtacgatga tctttttcgc aatggaaccc ctggccgtgg ccactttcac aaaataagtc
    74221 cccgccgcaa gatccccggt ctctaaccct atcgtcccct ccaccctgga tttgggcagg
    74281 accaggtgcc ggatctggtg tccaagcagg gaatacatat cgattctcac ttcgtccctg
    74341 atgagatcgt taaactggat attcacgcgg ttggtagctg gattgggata gatcgtgatg
    74401 gaaatgggtt ggcgttcatg ggctatgctg actccggaac atttccattg gttaaacaat
    74461 acttcgccac cggtatacag gttacaaagt gaatcggcat tggggctgaa caatgcaatt
    74521 gatctgacaa tacctgtggc aggatgggca agatcaaacc aggtggtacc gtcccatttt
    74581 gccaagagat ttatattctg gttgttgacc atggtaaact gtcctcccac atataattca
    74641 ttatttatca ccttaagggc atatatcccg ttgccattat ttacactgct gcctgagggg
    74701 ttgggtccaa tccctaaagt agtccaatat gctccattcc attgtgcgat atggtgcgtc
    74761 ttatttccat ttggaaccgt ggtagaaacc gcactgggaa attggcccgc cacaatcaac
    74821 agatttttat atgaagcaat ggcatgcact ccatcccctg aattaggtgg cgaggctggg
    74881 atatttactg cccccccgcc tcctaccttg gtccaataac caacccaaat ggctacgttg
    74941 ttgaagccac ctgccgaatt accaaaccgg ccacccgcca cgatattgcc ataaaatgta
    75001 gataatgcaa acacgggtcc attaaaccca ttaccaaatg tagcccagcc aggcccggga
    75061 ttccatcttg caatattgtt tgccgcaatt gagctggtgc caacagaatt aaagagtcca
    75121 cccacgacca gcccccaggc ggttgaaagc aaggcatcca cattgctcat agttccatta
    75181 atgcctcctt gtggaatgga cgcccaattg accccgttcc aggatgcgat tttatttgcc
    75241 gggatattac cggccatcgt aaacgctcca cctgcatata atatcccatt atgaacttct
    75301 atatcattta caatagcatt gagtcctcct ccgggcaagg gagcccagtt tgtcccatcc
    75361 caagatgcaa tattatctac gggtatgccc gggttgccta tcgtgctgaa ggagccagca
    75421 acaattaatt tgccctggta cactaccatg tcctgtatcc aagaagtatt aagttggttc
    75481 caggaagtac cgttacaaac agcggtacaa ctatcagaca ggatacagga tggttgaaat
    75541 aatattattt tacacgagca tatctgcatg ccgcaaatcg tttgcaaagt catggtagaa
    75601 tatcctgtat ttaagggtcc aggcaggttg atttgaaaat ttccgggtaa aacctggcca
    75661 gaaagattgg tagcaggtcc ggcaagcgtc cattgaatat ttggagtcga acaagagtca
    75721 ccaaaacagc taaaaatgcc actgaatgaa aggggattgg gtgcaggaca tcccatggta
    75781 atggtatcgt tacaatgaac cggtatcaac gtaccatttt gagacatatt catggtatac
    75841 ctgccacatt cacattggca gtgcagatct acagtaaact ttaagagggt atcaaaacac
    75901 ggtgtaccat tcgaatcaaa ttcgacaatg ggaatttgaa tggtatagtt tccactctgc
    75961 ccgtatgaat gcatccacat accggtactg aatggcccaa aatcgatttg cccatcgccc
    76021 caactaataa aatatatcga atcacaggga ggcaatcgca acacattgat ctcgacctta
    76081 cataaagaat caactacact gagatgcacg gcattcattg cattctgtat aaaattagcc
    76141 tggcttatac aacaactgtc cacctcaggg ggacagttgg caaaatcaac ctgagccacc
    76201 tgattagcat ccaggaaaat tgaggacata ccggtagcag gtaatgaagg cgtccaacca
    76261 ggttgattta attcttttac ggtgtataaa ccaggcagta aaggactaaa aatataagac
    76321 ccattctgat cagtagtcac tgtatcgata gctactcctt gttgattgca taatattatt
    76381 tgccatccct gcaaggtttg aaggcttcct gtataggacg taccggtaca aatagtatcg
    76441 atatatttaa taccggtgat cgccgacggt tcacaacaaa tatctttacc cagtccctga
    76501 ccttttatac tgccacatac attgacgccg gtaggactcc cgtcaaaatt atatacattg
    76561 gctttaatgc aatacctgcc tttatccaaa atcaggttga ctatggtatc ccgtgtaggt
    76621 aagtgaaaag gaactccggg agaactgggt acagacaaaa gttgacttac cgtagttccg
    76681 gtgctgtcac aaaggatgaa attggcagaa ttatctgcca ggacactgag cttgatctgt
    76741 atcatcgctc catcggagca aacacaaaaa cacctttgga aggaaaatat tccctggtcg
    76801 tgaccatatt taaacgcata caaagtgagc cattttgaac aaggttggct gccattgggc
    76861 tgatcgtccc aaaacatggg gtcggtgtag gtcaccaatg aaggtatggg caaattcagg
    76921 ttggagtacg gagtttgtac aatagtccaa tatggatcat aacttccatt cggataaaac
    76981 tggccggtgc tgtggtctat gcccgtgctt agtgaaatac tgtcagataa acaggcgggt
    77041 ccgcaggtgg tatccggctg aggcggacag aaaaacacaa agctggtatc acaacttacc
    77101 gtggtctgtc cgatgaggta ttcggcttta atctttatcg tatccggatt agaaccattg
    77161 tttatgcacc agctgagggg ggtaaaactc ccggttggaa taaagccgga agcgtgcgtg
    77221 aggctgatat tgctgagact ggactgactg gtaaaccagc cattgagatt atctaccgta
    77281 atggaattgt aggaggcgat gtttagctcc agcggtattt gtgtaatctt attcttaagt
    77341 ttattatcaa tgtgcagccg gaaacaacag gccggtggaa cggtccttaa cgattccacc
    77401 cagaatgaaa tgctgtcaca ggtggtgggt ggacaatttc caaaattggc ttgctggact
    77461 tcgtgagggc ccagggcaac gggcaccagg ttgttcgacg ggtaggtgag ggtccagccc
    77521 ggttgattaa tttctttcac ggaataattt ccaggcgtga gcgggccaaa gacataagat
    77581 ccgctggcat cggtggtgac cgtatcgatc gtatttccgc ccgcatcaca taaaataaac
    77641 tgccatcccg gtaagacggg ttggcctgca taggcctgtc cggtacaaag agaatccctg
    77701 tatttgatcc cggtgatata ggattccggt ttacaacaat cgtcccggat cagtccctcc
    77761 ccgcacacgc tgccgcaaac actgatcccg gtaactcttg tccagttttt aactccggcc
    77821 cggatgcaat aattgccttt tttcaatacc aactgggtgg tggaggtatc cgggggcaac
    77881 aggaaggcca gactggggtt gttgcccggc gggtgaatgc ccaatagggt ggtcaataaa
    77941 tttcccgatc cgtcatacag ccagaaatcc gcttcattat ccgccagggc gctcaatcgg
    78001 atggtgatga gggcactgtc cttgcatacg cagaaacaat tggaataggt atatctcggc
    78061 ccggaaccct tgtccgcacc ttgaaatgcg gaaatccatt gagcacaggg tgtacttgca
    78121 ttgggctgca tatgccaact gacgtatggg gcgaccactt cagcgggccg gggcaggttc
    78181 aagccggtat acgggctttg aatcaaaatc caattgggat caaatgtccc tgtcggaaga
    78241 agctgcccat tctgatctac gcccgtattc aggctgatgg tcgtggtcgc gcaggatggg
    78301 gacaagccac aggtggtatc caccgggcaa ttgaaaacaa tattggtatc gcaagtcgac
    78361 agattgttat tataaaaaaa cctaataacc gcactatgcg gattcgaaac gctgttgatg
    78421 cccaggtctg ccgcatgaaa acttccgggt ggaatgaatc ccgaaggatg tgtgatggtc
    78481 aggcgattgg cgggtgaaat caccgaaatg aagggagaaa caatccccgc tgaattaaag
    78541 gaagccgtgt tgaggtcaat ttgaatcttg gtaattcctg aagcctggtt gtttacatga
    78601 aactgaaaag tgcaggcata ggggatggcc tgaaaaggaa ccataaaagc ggaaatactg
    78661 tcgcacttgg gccgcggaca tcttaccatg atttcaaaac tacaggtgct ggtattccca
    78721 cccatatccg tcacggtgta ggtcaccgtc gaaagtccct gtaagaaatt tgtcccgctg
    78781 gcatcctggc tcccggacgc aaccgtcgct ccggagatga cataagtggt attcagcatc
    78841 ggacaattgt cggatacagt aggggcaata ttgttgacca caaccttgca ttgcccgaat
    78901 gcatccaggg atccggtcaa cgaaatattg gccggacaac tgatggaagg aggttggttg
    78961 tccaccacgg tgagggtgaa attgcagttg acgctttggt tgccgcagtc gtctgtggcg
    79021 aatacggtga tggtgttgac cccttttgga aactgggttt gcggggtaat taaaatcgag
    79081 ctgcccgaag cgagcaggga ataagtcacg accaggtttt gatcacagtt atccatcgca
    79141 gtggctgatg gaagggctcc ggtgtaataa caggtgcccg gatgtgtatt tacggtaaga
    79201 ttggagggac aagtaatggt tggaggcaca ttatcctgaa tgaggatagt ctggttgcag
    79261 cttgaggtat ttccacaagc atccttggcc tgccaggtgc gtacaatagt tccattgcag
    79321 ggcatgaggc cggtgaccac atccgaatag gtgattacaa tggcattgga tggggtgcaa
    79381 ttgtccgttg cggtagccgt acctgttgag gcaggagccg tgttggtatt gcatctcagg
    79441 gtcacgtttg ccggacaggt gatgaccggt ttgatcaggt caaaataagc cacagtctta
    79501 cagatggaat ctacacagcc tgggcccgtg gttttgagac aaacgataaa atccccgcat
    79561 ttgctgaact ggtggtttgg gttttgggct gttgaagtgt tggctgagcc gctgcccgga
    79621 tcgccaaaat tccaggagta ggttaaaggg gcagggccag tagattgatt ggtaaataaa
    79681 aaattaccgc aattgctgat gctgttaaag gaaaaggccg catcgcatgg caaaggatct
    79741 gaacaaacct gtaccggaat gttgcatgat ccggaagtac ccagggcggt atagttgaaa
    79801 ctgatgttca gggcatccgg attattccct cccatcaggc agaaggtcag cggactggcc
    79861 cccccgaccg ggataaaccc tgaattgtgg gataataaga tatccatctg cggggtttgg
    79921 ctgaccaccc agcctgcatt gacctcaact gcaatgttct tgaaagtcgc cgtgctgatg
    79981 tccagtttga tctgggtgat ggcatttcga agtttattat ccagaatgaa ttgaaaacag
    80041 caggaagggg gtgaggtcct caattgttga acaaggaagg tgatgctgtc acaggccgca
    80101 tcgggagcca gggaaccgct ctccgctacc ggcataacct ccttaaaagt ttgcagtctg
    80161 aacgctgttt tgcctgaagg aaaaaaaaca attacacagg ccagtgtaaa aaggtaaaca
    80221 aacttgatca tggagtaagt ttatggggtt gatcctgtcc tgccggatgg gcttccttcc
    80281 tttaatatcc ctcacagcga ttcaggcaat atctacaggc ttcgcatcaa gccatgctca
    80341 aacacactct ttttaatatt cgtaccattg aatatactaa accaatcctg gaatcccaaa
    80401 atgatttcta tcaggtcccg gccacgggat ttcatctttt aaaagtaccg aataaagatg
    80461 ccggctattt tcttcttatg gcctggagag cctgccttgc ttcttcggtc agcagcagcc
    80521 tgccactggt gccggcccgg tccagcaacc atttatccca ttccggctcg ccctcccaca
    80581 tcattttttt gatctcagtg agcgcagggc ggctgtatcc gctgagctca tcgattttat
    80641 tcgcaatatg ttcatccatg gcctcggtaa tatgaaagac ctcgttgtac aggccatgct
    80701 ccatggccca ggaggcggta aaccaggaag ccgggttcaa ggtcaaatga ttaaaggccg
    80761 acaagccggt cttgcgttta atggccgggc ccactacaaa agggccaatg ccattgatca
    80821 gttccgcgag acggatttcc gcctgatcgg tcgcataact caaatccgag gcggcgatca
    80881 ggccaagccc gcctccgacg gctttaccct ggacgcggct gatcactatt tgaggagcgt
    80941 tccggatggc caggataagc cgtgcaaact ggaaaaaaaa ccgacgccct tcttcctctt
    81001 cttttataga taacaattca ctgagatcgg cacccgcgca aaaacttttt tctccctggc
    81061 tccggatcaa gaccagccgg atctccggat cctcactgac tttctccagg tgtgccccca
    81121 tctgttccag gacgccggaa ggcaaggcat tatggaaagg ggttccgaat tcaagggtgg
    81181 ccaatggacc ggaaagggat aaatggcaat aggacgtcat gataaaaacg aataagaatt
    81241 taaaagaata agtttaacac ttcctgcgtt cccagccaaa acctgaattt ccaatcccat
    81301 ccatcttacg aatcctataa tcctacccat cctattaatc ctactaatct tattaatcct
    81361 accaatccta ccaatcctat caatcctacc aatcctatta atcctaacaa tcctattact
    81421 gcttcaccgt ctggtccagg ggcttaccca actgaatata gagattttta cggccttcct
    81481 tatccttgga taaaatctca tgggcgaatt cagcaacctg gagtgcatgt tccctgggta
    81541 ccgagatcac cccatctcca tcggcaacca atacatcgcc gggccttacc aataccccac
    81601 ctatggtcac cggcttgttc accgactcga tttcatttct tccgggccgg attcccctgc
    81661 cccgctccat ataatccagg taaaccggga tttgttgttt gatcagttca tccgtgtccc
    81721 ggatgcctcc attggatata atgccccgcg cgcctttgga aacccaaaac aaaccgttga
    81781 acgatcccac ggaacctacg tccccatcac cctggacatc caggacgacc acggaacccg
    81841 gtttaagaaa ttccacaaag ggctcagtac tgatttttcc gtaccagttc ccctcccatt
    81901 tctggaattc ctctttcggc atcggatttt tcacaatttt agtggtaggc acgtacctgg
    81961 cggtcaccgc aatcccgcag aagatgtgct tcatgctgtc aatgtctttc caaagcgctt
    82021 cgatgcgggt atccataagg cctgcgtcac gaagccccac catgtccata ccatcagaga
    82081 catcagccac gcggaggcct tgaaacgctt taaggatctg ttcatcgctg accgcctgct
    82141 gggcggaggt catgcctgtg aggaagatca aaaatatggt aatgcaattt ttcataaccg
    82201 ttggatttga tacttaaata tagggaatta cagggtacgg ctaccgggcc aatcccggag
    82261 aaatggcggt cagccattca gacaatcgtc ggccaggctg attgggtcgg tacaatccca
    82321 gtaatcgact tacctattga ccagtttttc cctggtttcc agcaccactt ccaatccatc
    82381 cgggaatgat tacgggaagg gccgggagtg tgatccgaga atgtcggttt ctaatcgaca
    82441 ccatttttct ttcaaggctt ggacgagaga ggcaggacat gcatgtttcg tggttcagcc
    82501 agagcttcag ggaattacaa tttcctgcga tgaagatcct gttcgtaaaa tatatttacc
    82561 ggcaggccat tggctgatgt cgatatgctc atcgcggtaa aactggttta aataaaccgg
    82621 ttgtccaccc tcattgaaaa tctgaatggc caagggcacc ggtggatgca tgcttaggga
    82681 aagggctgct tttccggcga ctttaacgat cttcaagacc ggagcggatt ggaccatggc
    82741 cgataactta ttctcttttc caaaactgtg ggaacctttt tggaaggggc ctgatccgtt
    82801 gggtagcctt gaataggcaa tatcctcctc catgggagga atggtcaaac tatccaccag
    82861 gctcccagtg ctatccaaga ggaacaatgg ttctccgaag gcggaaagtt taaaattggt
    82921 atgcaatccg gcttgtttgc cgtcctcatc cgaccaaagc accaggtaac ctttgccact
    82981 tattttgatt ccttccggaa tccggtattt taccaggttt gcaggattgt cggtgaggat
    83041 ccagcggctc aaatccacct ctttttccga gttgttgtgc aattcgatcc aatcatcaaa
    83101 ttcatggtcc tggtccgcaa cggacaaagt attggccgac atgacttcat tgatcacgat
    83161 gtttttattt tgacgctggt cagaacctac ccggtagcaa taaacatcat tttccgcagc
    83221 cgggggatcg tatgtggccg tcttaaaatc atcggccgca atggcttcga gataaaatcg
    83281 gacgaattca ccgccttccc gggcgggtaa aacggctccg aataatccat cgagggcctc
    83341 accgtcctgg tgtttcccgt catcttccat ccggagcctg ctgaaaggtc cgtccacccc
    83401 ggtcccgaca taaatataga ccgcatcgat gggcgtcggg cctgaaaccc tggtgttcac
    83461 aacgactcct tgcccggcaa gcggggcctg gaaggctacg ttcctgaatt tgtattccgt
    83521 atcggttatt ttcaatcctt cccggttcac ctccggatgc gtcaggatat attttttccg
    83581 gtggtccatc ctgattttca aatcttctat ggcttcgata tatgaggaat agggatacag
    83641 ttttttatta tccttcgcca ccaccttacg aataagtgaa tcataccgct caatcatcgg
    83701 aagatagacc tcttgcctga acagatcttt ggtcatggtc cggacgtgag caagataacg
    83761 ctgacgaaat tcgggaatag aaaaaagttt ctggcacagg gggaagaggg ggtcctgttc
    83821 gcgcataaac agattccagt tgccctgggg ctggtcaaaa aaaatttgat tgttgtcgta
    83881 ttgaataggg ctcgccctgc cggacttgtt atccaaataa agaaagtagt ccatgcctcc
    83941 atcgaacaca taaccgttgt ggtcttcgaa tacaatttcg cgggccagga accacaaggc
    84001 cctgtctaca tccagcacgg aaagaatact atcgggacgg tcctggttcc gcaaagtacg
    84061 ggtaagtgaa ataagatatt tccaggggtc ggccatttta cttcgcttca gggtgtagtg
    84121 cttttgatag accaaactgt cttctcccat gaagttcaga ctggtaaaac cgggtttcca
    84181 ctgatccacc agcccggggc tttccgccct ccagctgacg ccctcattat ccgggaacca
    84241 ttgcctgaga aatctgccgt ccaaggcctg gatgttcgaa taaagccccc aaggggtacc
    84301 attcagatac aaatccacaa aagaggcctt caaggagggg ccatacttcc gggtaaggtt
    84361 ttcgaaaaag acttctttaa tgaaactggg atcccaggct ccgttgtgca ggttaagagt
    84421 ggtatacccc atcaggtttt ggctttcatc cacaaaatca agggtgacgt tcagggattt
    84481 tttatacttc gttcgtgcga tatggaaaga actgtaccct ttataacgga ctccaacctc
    84541 cggatacgtt ttgccttcca tgatcaggct agccgggatg tcctttttga cgggataatt
    84601 ttcaaataat agggccggcc agtcctcctg actgaagcgg agatctaccc ttcttattat
    84661 gcggtcatca taaagacctt tgacttctcc gtttcccctg atgagtctcc gaccatccgg
    84721 ggtgatgccg tattcttcgg ggaggaactg tcctgagagc ctttgaccgg caatgagaaa
    84781 gaggaatatg tacaataata ttttattcat gcggactcat aaatttataa atctttcaga
    84841 tcccggcgat caaggggcct gtgagtatta cattactata ccctttgccg cctcatggag
    84901 ttcggaaacg aattatggca aaggaccatc cttgaagggt agcgaaaagg gccaacaagg
    84961 caaccagcca cttgtcgtac ctgaaccggg ctgcactggt caggcctgta gccttttttt
    85021 cagggcaaac aattcatccc ggtactgggc cgcgctgata aaatcgaggt ccttcgctgc
    85081 cgctttcatt ttcttttctg tttcggccac caatttctcc aactggtccc ggccggcata
    85141 ttccagcacc ggatcggcgg cttccagtcc ccgatcctgg ggttcgaggt aggcattcct
    85201 gggtccacgg acatccagga tggaccgctg gcccatgatc tcttcacggg tcttggcgag
    85261 ggaccggggg acaatcccgt acttctcgtt ataggccatc tgtttcaccc tccggcgttc
    85321 cgtctcatcg atggtccgtt gcatgctgtc ggtgatttta tccgcataaa aaatgacctt
    85381 gccgttgaca ttccgggccg cccgcccggc ggtctgggta agcgaacggg cattgcgcag
    85441 aaaaccttcc ttgtccgcat cgagtatggc taccagtgaa acttcgggca ggtcgaggcc
    85501 ttcccggaga aggtttaccc ctaccaggac gtcaaaatta cccagacgaa ggtccctcaa
    85561 aatttccacc cggtccatgg tttgaacttc cgaatggata tacttgcaat tgatattgag
    85621 ccggcccagg tatttactta attcttcagc catccttttg gtgagggtgg ttactaaaac
    85681 cctttctcca gactccgccc gcttgctgat ttcttccatc aggtcatcga tctggttgag
    85741 gctggggcgc acttcaatcg gagggtcaag caggccggtg ggcctgacga tctgttcgac
    85801 cacttcaccc attgtttttt caagttcata gtctccgggg gtggcgctga cgtaaatcac
    85861 ctggttgacc aggccttcga actcctcaaa gctcaagggc cggttgtcaa tggctgaagg
    85921 aagcctgaac ccaaagttga ccaggttgag cttccttgcg cggtctccgc cccacatccc
    85981 cctgacctgg ggtaaggtgg catggctttc atcaatcacc atgagataat cttcgggaaa
    86041 ataatccagg aggcagaaag gccgagtacc ctgggcgcgt ccgtcaaaaa accgggaata
    86101 attttcgacg ccgctacaat aaccgagttc ccggatcatt tcaagatcaa aattcaccct
    86161 ttcgtgaatg cgtttggcct ccaggtaacg gccttcggac tggaaaaatt tttcatgttc
    86221 atgcagttcg tcctcgatat cccggatgat gtacttcagc cgctcccggg gggccacata
    86281 aaggtttgcc ggaaagatcg ccgcgttgtc catcggctcg atccgttttc cggattcgat
    86341 ttcgatttgt tcaatggatt cgatttcgtc accgaaaaat atgacgcggt acccataatc
    86401 cacataaggc aggtatatat ccacggtatc ccccctcacc cggaaattac cccttttgag
    86461 gtccagatcg gtgcgtgaat acagggcttc aaccagttga tgtaaaaagg cgttcctgga
    86521 tatccgttta cccttttcga tgcgaatgat cccaccctta taatcctccg ggttgcccat
    86581 gccatagatg caggatacgg acgccaccag gatgatgtcc ctgcggccgg acaaaagcga
    86641 agaggtggcc ctcaaccgga gtttgtcgat ctcctcattg atactcaggt ccttttcaat
    86701 aaaagtatcc gtgaccgaga tataggcctc cggctggtaa taatcgtaat aggatacaaa
    86761 atactcgacg gcgttttcag gaaaaaaatc ccggaactca ccgtacaact gggccgtcag
    86821 ggtcttgttg tgagaaagga tgagggtggg tttttggacc tgctggatga cgttggcgat
    86881 ggtaaacgtt tttccgcttc cggtgactcc gagcagcacc tggtgccggt ccccggattt
    86941 cacacccctc accagttgcc ggatggcttc gggctggtct ccggtgggtt catattttga
    87001 ggtgagtttg aaaggcacgg ccgcaaaatt accgatttac ttttgacgaa gtcttcgatt
    87061 tccaaagttt ggtatagcct ctttccaatt ttgttccctt gaccctttac ccttcacctt
    87121 tttccctttt ctacttttgc tcctttcgcc cttcgccttt ttcccttgac ctttcctccc
    87181 ttcctccttt tctcccttaa aatatttgag tcccagggta accttcccta acttagggag
    87241 ttaacctact aaccattgtg attgaagaga cagacaacga actgatgtac caggtcaaat
    87301 ccggccggtt ggaccggctg agtctcctgt ttgaccggta ccaggctccg ttgtttggat
    87361 ttttcaggag cctgacaagg gatgcggagc agagtgcgga cctggttcaa aatgtattct
    87421 accggatact taaataccgc aaccagttcc agggaggcca gttcaggacc tggatgtttc
    87481 acatcgcccg gaatgtgcac cgggaccagt accggagaaa aaacaaggcg gtaaaggtat
    87541 ccatcgatga ctaccgggac cagttgaaag aggaggtgga agccgacttt gatttacata
    87601 cgagggaaca gcaccaccag ctccggaagg cgatggatgc cttgcccgaa gacaagaagg
    87661 agatcctgtt actgagtaaa taccaggaaa aaaactataa agaaatcgga gccttgctgg
    87721 gctgcagtga agggacggtt aaagtaaggg tattcagggc cctccacgaa ctcagggtcc
    87781 tttaccatca acttgaaaaa aaggaaatct tatgaacgaa accgaattaa aatacctggt
    87841 cggacaatat cattccggcc ggatcagcca ggaagaatgg caaaaattgg aagaggccat
    87901 tgcgcagggt cgaatagacc tgtcagaaat acaggtcctg aacgaattga gcaccagcat
    87961 cagccggctg gaagacccga aaccgccggt tgaaataaga ggaaaggtgc ttgaaatgat
    88021 agaaaacgaa aaccgactga tcaaccggga agggatatgg atcaaccggg aatggctgat
    88081 ccgcaccgta ttggctgccg cggctatgct cgcgggtatc ctcgcaggct accacctgcc
    88141 ctggaagagc gctccctccg gagatgtcga agccttgcgc ggggaagtgg ccgcccttaa
    88201 agaaacgatg cttctgaccc tgattgaaaa agaagcagcc accgaccggc tgaaggccgt
    88261 aagttatagc tatgatctgg accagggcag ccgcaaagtg attgaagccc tgctgaccac
    88321 gctcaatgaa gacaataata tcaatgtccg gctggctgct gtggaagccc tgcaacgtta
    88381 tggatcggaa gactgggtgc ggacccaact ggtcaaatcc atcggccggc aggattctcc
    88441 cctggttcag atggccctcg cagaatgtat ggtggccttg aatgaaaaaa cctctgtgca
    88501 ggaattgcag aagctgatcc atcgcgaaga aaccccggag gcggtcaaag acaagctcaa
    88561 aggctatatc cggcagatca tataaccatt aaaatccata aaaaatgaat aaacccgttt
    88621 taatacaggc catgttatgt ctggttctga attcaagcca ggcccagtcc ttcaccgaaa
    88681 agatcagcaa aacctattcc tttgaaaaaa aagggagcca gaatgccgtg atggtattca
    88741 acatcaatgg aaacgtcacc atagagggca cctccgggga tcaggtcctc gtggaagttg
    88801 aaaaaaccat ccgggggaaa accgatgccc ggcttgccct gggtaaagaa aaaatacagc
    88861 tcggggtcat ggacctcgcc gacaccctca tcctttatgt caaagggacc ggcgctgaat
    88921 tttcaaggaa ttccaaaaga aaaggcccgg aagccggggg ctggggttat caatgggaga
    88981 ggaattggaa tggtaataac gacaatgaca atgaggatga ccgggatgaa tttgagtata
    89041 cgttaaattt taaaatcaag attccttctt ccgtccatat catggcctcc accataaaca
    89101 acggagatgt ggtggtcagc ggcacccgcg gcctcgtaca gacccggaac gtcaacggtc
    89161 atatccggat caaggacatc accggcggca ctctggccca taccatcaac gggaacgtgg
    89221 acattgatta cgcgtccaat cccaagcagg attgcaggta ttataccctg aatggggaca
    89281 tccacgtcta ttttcccacg acggtatccg gttccatggc ctttaaatcc ttcaacgggg
    89341 acctctatac gaatattgag ccgctgacac ccctgcctgc ggtggttact aaaaaggaat
    89401 ccgaaaaagg gatcaaatta aaagtggaaa aagaacgttt ccggatgcgc ggcgacgggc
    89461 ccttattgga tgtggaaacc tttaacggcg acgcctatct gaaagaaatg attaataaat
    89521 aattcaaaaa tcaaataaaa tgaaaaattc aacttggata ccactggtcc tgtttatgtt
    89581 tgccttcatc gcccttgcgg gcgcgcaaag cggcaatgaa ttcaccattc ccctcaaaga
    89641 ccctgccaaa aggggaaaac tgtacgccca tctcaatgcc ggttcgatca ccgtgaaagg
    89701 cagtgcacgc aaggatgtgc tcgtcaaata ttctgcggtc tccgacaaag aagaaaaagc
    89761 ggaaagcaag gaaggccttc gccgaatcag cggaagcagc ctggatctca ccgcctctga
    89821 agacgacaat aatgtcaagg tggtttccaa ttcctggaat gaagaaatca agctggagat
    89881 tgaggtcccc tcgggctttg atgtcaaaat gcacacctac aacagcgggg acctgaatat
    89941 ttccaatgtc cagggcgaga tcgacctgac caattacaac ggggccattt atgccagcaa
    90001 tatccgcgga tccgtcgtag ccaccacgta caacgacgac atcaaggtca gcctggatga
    90061 agtcaaggcg ggggtcccca tggccttttc cacctacaat ggcgacatcg acgtgaccct
    90121 tcccgccgcg gcaaaagcca cgctcaaatt caaaactgac cagggagaca tttataccgg
    90181 ttttgatgtc gacatcaaaa gttcaggccc ggtgaaaaaa gaggacagta aggcgggcgt
    90241 gtacaaggtg atgatcaatg aatggaggca gggggatatc aacggaggtg gcgctgagtt
    90301 taccttcaag aattataatg gggatattta cgtaaggaaa aaatagacta ggatttatag
    90361 gattggtagg atttatagaa caatcctacc aatcctacca atcctaccaa tcctaccaat
    90421 cctactaatc ctactaatcc tactaatcct agcgaacaag attgtccgga tgtttaagat
    90481 tataaacccg tttatactcc aggcttgaac acccaaaatt aatcagtaag ccataggaga
    90541 atttaaacat ttctatatag ttcatgacct gggccaaaaa agaatcctca agtatcgcaa
    90601 ctgccttaat ttccaggaca agccaaccct ctatcaaaaa atccaccctg cgctctccga
    90661 tcaagatccc atcatagtag acttgcatct tgtactcccg aatgaagcgg atcccctgtt
    90721 ttgccaattc aatggccaag gctctttgat aaatgacttc ctgataacca ctacccaggg
    90781 tggcatgcac tttcatggca cagccaatca ctttatatgt caggtcatcc tttctcacag
    90841 tgtattttag cgtgtggcga taaaattaag gatttagact gatcaattca ctcgccgacc
    90901 ctaccaatcc taccaatcct attaatccta tcaatcctac caatcctatt aatcctatca
    90961 atcctaccaa tcctactaat cctaccaatc ctattaatcc tattaatcct attaatccta
    91021 ccaatcctat taatcctata atcctattac ttccacacta ttcaattccg gccccccacc
    91081 ccggttccct gctcccccca cgatataaat tttattctta accctcactg ctccggtacc
    91141 atgcctgccc tggttgagtt ttgcaaagga attccacttc attgttttgg tatccaggac
    91201 ctccacttcg ctgtgcgcgg gaacctgggt atcgctttcc ccaccaatta caatcaattg
    91261 atggccgtgg gccaccacag gtgaaccggc cctcagcgtt ggaagattgc tggattcggg
    91321 aagcgtggtc caggtcccgg atttaaagtc gtaaacatcc acggatgctt ctttcaggtt
    91381 gatcacattg ttggtccggg cggtcgtacg ccgtccaccg gctacataaa ttttgtgatc
    91441 gatgacctca atgcagacat gatcgcgcgc atggggggca tcggccagcc gggtccaggt
    91501 atctttggca ggatcgtatt catccagcca gccggtgtgg ccgtcccaat ggccatcctg
    91561 ggtgcccccc aggatataga tcttgttttt ataaaccgtg gccccggctg atccgcgaag
    91621 ccggtctttg ggaatagccg gacccttcct ccattccttg agttccggac tgtaaatata
    91681 aatatggtca acaggcagct catgagggta attcccggta aaggcgccgg ctacccagat
    91741 ctccccattg tacaccacgg cctggaaatg gtggagttca accggaggcg aaggcaaagc
    91801 ttcccaggcc ttggttttca ggttgaggac atttacaggt cggatccccc ggctcccgag
    91861 gagatatatt ttgtttttca catgggccag ggcgctttcg ctgcgggcgg tgcagtgtcc
    91921 ggaggtctgc atggtttccc agccctgggc cgaaagaaac aggggaaaga gcaggaatcc
    91981 cagggcatac ctcaacccgg atattttatt catacttgta aaaataagac ggattcttca
    92041 tcccggtaaa atcaatcaat cttagcttct gccgcctgta acacacggaa tgcaggggag
    92101 ggttcgtgta taaaagcgat caggctcaga tcaccggttt tccagtttcc ggatagagtg
    92161 gtcgagtact caaaagtctt tttgacgttt gtgggcagga tcccggccgg atcaccggaa
    92221 atggcgctga ggaatgaacg caatacatgc cggtggacat aattggtatc cacaccggtt
    92281 ggggttagct gggcatcccg gatattagtt tctgtcagcg cgacgcttaa ggacaaaacc
    92341 tggctctgaa cctccagcgc ccggacgtgg actttaacct tgagtgcaag cggatccggg
    92401 tcgggtagaa tctcaagctc caagccgatg atcggttcct ggtccagttc ctcagccaca
    92461 taccccgccc agaatccccc tcccagaaac cgcgaagatt gcccatcgaa aattttacgg
    92521 ctgatcatgg cggatgggta tcccagcggt tcattgaaga gccggatcaa ttctgcccca
    92581 aaagcattgt ccagcc
//
LOCUS       DAOQXT010000002        89280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_237_length_89280_cov_7.936105, whole genome shotgun sequence.
ACCESSION   DAOQXT010000002 DAOQXT010000000
VERSION     DAOQXT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 89280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 89280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89280
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_89280_cov_7.936105"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..765
                     /locus_tag="PKM27_00245"
     CDS             166..765
                     /locus_tag="PKM27_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNR05707.1"
                     /translation="MSENRLIQLQVRSLKLFRKIHRWTGISLFVFFTIVALTALILGW
                     KKNTGELIMPATKKGSTAELKAWLPLDSLHKIACQALHDSVSGALSTELDRIDVRVDK
                     GIAKFLFIERYWEIQLDGATGKILSIGKRYSDFFENVHDGLILDRYLGTSGEPIKLVY
                     TTLMGLALLVFTITGFWLWYGPKRIRHHKHAQKTSLPHH"
     gene            complement(767..1618)
                     /locus_tag="PKM27_00250"
     CDS             complement(767..1618)
                     /locus_tag="PKM27_00250"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNR05708.1"
                     /translation="MRLYPGVLNALILAALTVTGSSQSGPKGSLFIIGGGDRPMAMME
                     KMLKTADLGPGKNILVLTMASANPDTSYHYIKKDLQVLCANPVLKMQLTRVSARQPAV
                     LDSIRCAGLLYITGGVQSRFMDSIAGTGVAEAIHAAYRSGAMIAGTSAGAAVMSRVMI
                     TGNQIRGDTLYEGAVDRMAEGNIETSTGLGLLEQVIIDQHFIVRSRYNRLFTLLHQYP
                     AKVVIGIDESTAILVHGRRAEVTGEGQVVVARRPVKIRTGPGRKIGFRSARLDVCLEG
                     DRFKIKP"
     gene            complement(1629..3200)
                     /locus_tag="PKM27_00255"
     CDS             complement(1629..3200)
                     /locus_tag="PKM27_00255"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR05709.1"
                     /translation="MAKCLTRLWAVLLVSTCISFPGLAQKKQSYAEPVYPEDDRLQRM
                     KAAMPFIDSLYRDYAKEKQFPSLAYGLVADGQLIASGCLGQANTNQRIRADLSTLYRI
                     ASMSKSITALAILQLRDAGRLRLDDPVDLYIPLMKNNSLLTTDAPPITIRHLMSHAAG
                     FPEDNPWGDRQLEDTHEDLVALIRKGTWFSNVPGVAYEYSNLGFALLGQIVEKISGQP
                     LEQYTREKIFKPLGMDHTEWEYTKVPQGKLALGYRRIGQEWIEEPLLHHGVYGAMGGL
                     ITSIGDFQKYLSLHLSAWPPRDGLENPVLKRSSLREMHLPVNFSGFHPAFRYPGQRPC
                     AVVTAYGYGLSWMRDCEGRVYVGHSGGLPGYGSQWRFLPEYGIGLVAFANLTYADLGT
                     INLRVLDSLIRRTGIRPRGVPASPVLLARQKQLIQVLPDWKAENLSALSSGAGNIFAE
                     NFFEDVFLETRQTIHQRAFKAIGPILRNGPLIAENQLRGSYLVEGEKGKLSVFFTLSP
                     EDPPLIQELEITIVK"
     gene            3319..4110
                     /locus_tag="PKM27_00260"
     CDS             3319..4110
                     /locus_tag="PKM27_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR05710.1"
                     /translation="MENKVMEYTVIEDHGFKYIETNPGKPVLLLLHGLFGALSNFKGI
                     LGYFENKLNVVVPILPIFELPITEVSLEGLVKHIKAFVTYKEIYSLDLLGNSLGGHLA
                     ILYTLKYPGEVRSLTLTGSSGLFESAMGTTFPKRGDYDFIKNKTELTFYDPSVATKEL
                     VDEVFNIVNDRMKGLRIVLTAKSAMRNNVENLVSSITAPTLLVWGKDDSITPAFVGER
                     FHELMPNSELVLLDQCGHAPMMEHPQKFNEILDGFLTRVHEVVYG"
     gene            4248..5150
                     /locus_tag="PKM27_00265"
     CDS             4248..5150
                     /locus_tag="PKM27_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR05711.1"
                     /translation="MKITRRSSIQKIMSLPLAVPGLSMRPRDPFGIPPVPGQRLRSSL
                     NAYSFNGPLMEGKMDIVGMMEYCALLGFEGIDITGYYFKGYPNVPDTDYLFGIKRKAH
                     ALAIEISGTGVRNDFTIPEKSKREDEVRRVFQWVEVAARLGAPVLRIFAGQEKREGYN
                     REQITEWILEDLRRCVDYGKQNGVIIGLQNHNDFIQNSDQVISIMKAIPSPWFGLILD
                     TGSYRQEDPYLAIEKTIDYAVNWQIKEKIFVGGKEVETDIPRLFSIIKKSAYQGYLPI
                     ETLGPGDPKPKIEALLEKVTREMQ"
     gene            5152..5625
                     /locus_tag="PKM27_00270"
     CDS             5152..5625
                     /locus_tag="PKM27_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR05712.1"
                     /translation="MDIVSQLRATKKQALLYFDLPPEVYDLTYCPGKWNIRQLLNHIA
                     DAETVLYDRIRRIIAEQPNPLVWSFDQDGWARHLNYDHFPLHINKHIFASIRDGIIYL
                     TGIYYEDHGTLPFIHSQTGARTLKDEIDKVAWHCRHHLDQIELALQAKPFISQVD"
     gene            complement(5802..7898)
                     /locus_tag="PKM27_00275"
     CDS             complement(5802..7898)
                     /locus_tag="PKM27_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNR05713.1"
                     /translation="MPRACFLFGILFFFSLNLDARVRVPALFSDHMVLQQNQQVPFWG
                     WSDPNATITISCSWDNVIYKVLADHKAQWRILVKTPEAGGPYTISISERNTLVLHHVM
                     IGEVWLCSGQSNMEWTASSGIENQDQAIQNSNQPLIRQIRIPRRVSDYPQEDIDAEWE
                     VCSPETMPGFSAVAYFFALELKERLNIPVGIIHASWGGSAAEAWTPKNIMETDPEFAS
                     WKEIFPENYPWPYLPASTFNAMIYPLVPYRIAGALWYQGESNTGNALVYRRLFPAMIN
                     GWRQAWNYNFPFYYVQIAPFQYGRPLQGSLVQEAQLKTLSLSPGTGMAVTTDIGNVQD
                     IHPRNKREVGKRLALWALANNYGQTLECSGPLYKSMKVEGQKVIVSFDHAESGLYIKG
                     EKPDLYRIAGEDRRFVEAKVEIDGNQLILSSEQVPEPVAARYAFTNTAEGNLYNHDSL
                     PASPFRTDDWPVVYSNILINSVYDSTSQGFLVSLEGENADKIVYSLNGTEPGLQSQVY
                     TGPFLVKQRTRVMAKSVSGDLLSENVSSREILMHKAAYRSVAYNNPPSEKYPGQDPLS
                     LVNGQKANLNAIGDKEWIGWEGNDLDVVLDLGEATRVRRIETGFLQNQNAWIFLPNQV
                     EISVSSNGRTYNEILKQTEEIRNDRNPLYKVYAADLNNTIRYVRVVARNIGACPPWHP
                     GSGSKAFIFADEISVE"
     gene            complement(8081..8152)
                     /locus_tag="PKM27_00280"
     tRNA            complement(8081..8152)
                     /locus_tag="PKM27_00280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8118..8120),aa:Thr,seq:cgt)
     gene            8361..9323
                     /locus_tag="PKM27_00285"
     CDS             8361..9323
                     /locus_tag="PKM27_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05714.1"
                     /translation="MKTLLNFWLLLLLVSSQFQVRAQSSDDRDSTGLPGDQFSLQGAL
                     EMFKKASSPEEFEKLINTENNHVNNLDLNGDGDIDYVKVIDHMDQDVHALVLQVAVSA
                     NENQDIAVIEIEKNGTESAVLQIIGDEEIYGEAVIMEPQGEEGKEKSRKGPSLLEDDA
                     IVINVWFWPSVRYIYAPVYRPWASPWYWGYYPNWWRPWRPLGWHVWHPFRVHYHVGYV
                     FAPTHRVVRAHRVYTPVRVTSVTVKTRNHQAVSNYRVNRSKTTVTATRGNNQVKASRT
                     QTTVSGKKGNTRGTATRTSTTVSGKKGNTKVNATKTRTSVRKRN"
     gene            complement(9414..9971)
                     /locus_tag="PKM27_00290"
     CDS             complement(9414..9971)
                     /locus_tag="PKM27_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05715.1"
                     /translation="MKNLLFLILFAAVNTGLSAEIPVRILSGTKLIIVEKNKWKSPVI
                     DVTIREVNGNVLVSESIKQSTKYNLKYVPDGEYVMEINDDQKISIQRLSISKGFLFSK
                     DSEIIFKPVVKIHDDKLDCNLMTQGNKAVLSIRNAQGDVAHSERFNNQVSVTRRFNLE
                     KLEPGNYYFDVSLSGRVFTKDFEVR"
     gene            10532..11044
                     /locus_tag="PKM27_00295"
     CDS             10532..11044
                     /locus_tag="PKM27_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR05716.1"
                     /translation="MQQVKAGDLSQASVLFRKYHRKIYYFLGKMSGDYHAAEDLTQNV
                     FVKMIQYKNSYNPEMNFESWIYRIARNSYLDHVRQKKNLVYTDLEPFQQLSEKDEANP
                     EEQYDRLHQALAGLPSDDREVLVLTRFQKMKYQEVAAILGTTESNVKVKVFRALEKLR
                     TSFFQLESNQ"
     gene            11044..11820
                     /locus_tag="PKM27_00300"
     CDS             11044..11820
                     /locus_tag="PKM27_00300"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR05717.1"
                     /translation="MNEQDLIREKLIDYIDGRLPEAEKGEIQRTLDENPAVREEYEQL
                     RILLHGLEEELPGIPSQKLIGRFEDWLDGYRSQARPESKSLFFSTWFKLAASLLLLVL
                     AGGGGYLIKSALDQRAEVAKLQKELAETKSLVLNQLQDKQSASQRILGVYAANELHTA
                     DDEIIKVLAKAMEEDPSSNVRMAALEALSRFYQDERVRTLLIGSLNRQTDPVVQIALI
                     QWLVQMKEKSILQDLEKITRGNSPVKAVKDEAYKGIFKLT"
     gene            11869..12648
                     /locus_tag="PKM27_00305"
     CDS             11869..12648
                     /locus_tag="PKM27_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05718.1"
                     /translation="MNTGKILFTLCLLGMAAALQAQEYKVAKTSGRLELHIGNVTVEG
                     HAGNEIIFSSKDGENEKDERAAGLVAINAMGMEDNTRLGINVSAKGDALVVSPLSKTK
                     SPEIRVLVPKGVIVSYDYESQYGGKARFKNLENELEISATYNGIELEGITGPVTVKTI
                     YSPIDAEFANTVKGPVSLISIYSTVDVTLPQSLKADLSMKTSYGEMLVAPEFKIEILR
                     PSEKEGDMVSMGGNKVQGKINGGGIKVDLRSDYSKIYLRKK"
     gene            12692..13429
                     /locus_tag="PKM27_00310"
     CDS             12692..13429
                     /locus_tag="PKM27_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05719.1"
                     /translation="MKKILFLLAAGWTSLCPAQSPVNKSIPVNSGQSLQLYFDYPNLI
                     RVSSWDKNEIQITGSVSINNGENDDAFVLTSAVSGGQVVVKGEIPDIKSLPQQITLRR
                     GNEKIVFKNKSEYEKYKQEHGASFDFMNRGSDIEISLDIKVPASLPVSVESVYGIVEL
                     KNLKQSMPLSAISTYGGVDAALHAAQVGELYASTDFGQIYSNLDVSFSGEGLKQKDFH
                     TEIMARPGKGARYRFESKYGNVYLRKN"
     gene            complement(13834..14793)
                     /locus_tag="PKM27_00315"
     CDS             complement(13834..14793)
                     /locus_tag="PKM27_00315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNR05720.1"
                     /translation="MPGPVFRTEIKLSPSPWKIQHQDRLFCIGSCFAEHLHLRFKAGG
                     FHSDCNPCGIVYNPISIGLQIRRILDGPAWNEQDLIHDRGLVHGLHHHGSFSSPRQED
                     CLSRMNDSLHEARDRFERMDVLILTLGTAIWYKHKASGQAVANCHKIPTSEFERGFMN
                     VEEVVQSVESWLPELLKRRPGIKILLTVSPVRYLKEGFGDNSISKATLVLAADQLCRR
                     NESIFYFPAYEILLDDLRDYRFYKEDMVHPNEQAVQYIWTCFQSTYFSKETRQIADEV
                     SRLVEARSHRPLHPGSPEHKSFIEKMETRAKDLKFKYPCLNLE"
     gene            14863..16191
                     /locus_tag="PKM27_00320"
     CDS             14863..16191
                     /locus_tag="PKM27_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05721.1"
                     /translation="MSFFDRIFSKKSQSADTLCYEASFGRYTDAYKSDAQFEAWDRAI
                     EQFEQQEYRKSLLDFIEYIKNPEGSNIILHSEQPVSFDMLQGSKTIECRLKSGVVFVE
                     CRIAAVSEMNIGFMRRLLDLNYELKYVRYGLTEDRVVVLRFDTPLTDASPYKLYFALK
                     ELSIHADKQDDLLLQEFSFLHPVRSGKIEPALPAEADHKLAFFRSAIDKAFHLVDHGK
                     LKTEQYPAATGYILLAAMYKLDYLIRPEGGLVDRIEQIHKSYFAQDQKNIQLKLKHLR
                     QEMVKLRQMSDDDLKAEIYHTISTFGITTPATLPVLHELIDRETQSIQWYIDQEYNEY
                     ALGMVEYIAGFSLFNFALPLPAKDLLHLIFRVTEAEFFKNIKGNQTLYDRGKISKAAV
                     RAELNRLESIHQKKYLALAFDHKKLRYDSPLTFAYGILQMVRGLQLQKAR"
     gene            16188..17273
                     /locus_tag="PKM27_00325"
     CDS             16188..17273
                     /locus_tag="PKM27_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNR05722.1"
                     /translation="MKDIIEIYKDYVVQIATPYNTGTGFLLPREGIIVTNEHVVRDNH
                     EVVLEGRLIPKQMARVLYLDQKFDLSFIQAPALAREVEGIEIEKTRRLHEGDLIIAVG
                     HPFGLKYTATQGIVSNTMHMQNDILYIQHDAALNPGNSGGPLINAHGRVVGVNTFIVQ
                     NGNSIGFSLPASYLEETLAAFSQGGGDEATRCESCSNIVFGKSSTGHYCLHCGSKIRM
                     PSDAEPYEAIGMQRTIEDTLIRLGYAVNICRRGPNNWELVHGSATIEIAYHDQSGLIL
                     CDAVLCYLPKTQIQELYEYLLRQNHQMQGGSLSLRGNEIILSTIMVDRQLDVQQGYHQ
                     IKNLMERADYYDDQLVNLFGAIWKQED"
     gene            complement(17277..18308)
                     /locus_tag="PKM27_00330"
     CDS             complement(17277..18308)
                     /locus_tag="PKM27_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05723.1"
                     /translation="MKKTLKSPLWILLLLIFTFGSLSAQNGHTKSIFDCVLEAEGVPV
                     IKIETNWNKLINQKMQEEYLPGKITLPCNGQPVDFDIRVRARGMMRKQVCFFPPVKVD
                     FKNGQLEKQALDTSIDKLKVVFQCRTGEANSEFLFREKLSYDLYRVVNPDYFVHHKLV
                     KFECYDEGKVKYTLDGLFVEDEGEIADRMGGKVIESGKVMVPALDKDNYLKMVFFQYM
                     IGNTDWSIPNKHNVEMVKVPAVAKVIPIPYDFDYAALVGTPYAVPHESLPIKSIYERY
                     YMGHGVTEEEALSTAKYFMSKKEELFKVVDGFTLLDEKSRSGIKKYLNPFFDLIESEK
                     VVKKVFVQN"
     gene            18672..19727
                     /locus_tag="PKM27_00335"
     CDS             18672..19727
                     /locus_tag="PKM27_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNR05724.1"
                     /translation="MKNCFYSCLLILLGVDLAAQVQRYLYVADDQGWIYIYDINKNHH
                     LVRKFEVPGTGEYKGISADAARERLYLSSYTGDQFVCVDLKTEKILYSIPILGYPDSH
                     AQTPDGKFIYLPKRHGRSWDVIDADQGKIVANIPVPYGNPHNTWAGKDGTRMYLAALG
                     NENLYIADTKTHSITGQVGPFQPNPEDPWGWTQKRHNGPKGIRPFAVSSDDRFCYVNL
                     DGILGYEIGDISTGKRIGRVGVEGFERKRGSHLTTSHGINLPPDEKEIWVSNDAGPYL
                     HVFDVTASPHKKIADIKLSGKNGWITFSIDGKYVYPSSGDVIDAASKTIIAKIIESEK
                     LLEIQFERGVPVRAGTR"
     gene            complement(19750..20601)
                     /locus_tag="PKM27_00340"
     CDS             complement(19750..20601)
                     /locus_tag="PKM27_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR05725.1"
                     /translation="MFIYKEYDQAGLDRQYNNRGLVPDFADHLNLWEQKSEEALRSCL
                     IYKDLPYGSGKPEILDLYPAPRPNGKTLVFIHGGYWQSMDKSNFRFVAPAFLPHGFHV
                     ILISYPLAPSASMHEIVASCAKALVWIADHIGEYGGDPESLYLAGHSAGGHLAAMMTT
                     VKFGPPGVRLKGICALSGLYDLVPIQLSFVNDKIGMDTSTAEEFSPVRLDPIPDSRIL
                     LAVGGDESAEYHSQLEEMDSAWKNKSVSIEKHLWPGLNHFSILTAFADVTSDFHQKTM
                     DWLTSNH"
     gene            complement(20620..21195)
                     /locus_tag="PKM27_00345"
     CDS             complement(20620..21195)
                     /locus_tag="PKM27_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNR05726.1"
                     /translation="MSGKILIITGDAGESFEVLYAKQRLSEAGYQAVIAAPAVKALNL
                     VIHDFEPGWDTYVERKGYLVTADTDFESVRPEEYVAVLCIGGRAPEYLRNNPRVIELV
                     RHFDQKDKFIFAICHGIQILTTAGLVGGKTVTCYEHVRFEVESCGGRYVDRDEAVQDG
                     NLVTGKTWQSHPDFYRLVIRSLNQTRKALAN"
     gene            21322..24072
                     /locus_tag="PKM27_00350"
     CDS             21322..24072
                     /locus_tag="PKM27_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNR05727.1"
                     /translation="MDPSESNIKDRFTIQKIYFLTGMVLFLIILESCRSTRITRPLAD
                     RYKVAADDSTLYADQAPYIMPYNRVLDPAGVSVPFGDGNYENHSLDAITLADGKTLVV
                     EDRYGLAVFDIHSRKLIDRWTFNRDSLFSGLMSSYSGITSIVHRDSTFIFWGAGGRNI
                     VIGAERANSFVMQASWDGKKIRGVHSYPFQAEAPARIALPNQVIAKIEEGELYFYTVL
                     NGNNKIVKVRVRDRQTIWETKTGMAPFGLEIIGDFAYVTNWAGPVPGPDSVERAGIPW
                     GEAYTDPVTGAVSRGSVSVVNIHTGRPTKEIEVGLHPNAILSSPDKRFLYVANGNSDF
                     ISVIQVSTNSVTDSIPVGLFGTEKGYIGSTPNALAVNQEGNRLYVSNGLDNAICIIDL
                     EETPSGKNYRVLGYIPTEAYPSGIVLHGDQIIVTNLESTGARASRTVQVGDKNSKSLK
                     TFNSHHQLASISFIPLPGAARLKRYTEQVKKLSMAHRLALTQRLPRPNIAPKPVPERI
                     GEPSVFQHVLYIIKENRTYDQVLGDMPEGRGMPSLCIFGDSITPNQHQIARDYVLLDN
                     YHVSGKGSAEGHHWASAGMVTDYTEKSVRAWFRSYPHVLNDAMVYNKKGFIWNNALDH
                     GKTVRIYGEACTYEYDKSKYNWKTVYELWENGRSSELAGKSTTTISRVRPILSQNFPG
                     GDDQNINEQMRADAFIKELNEFSALPGDQLPNLMIMALPNDHTAGTSPAFPTPRSMVA
                     DNDLAVGRIVEAVMSSRFAENTVIFISEDDSQSGWDHLSSYRTTGFVVSAYSRLKKTV
                     QTNYNQTSMLRTMEQILGLPPMNVIDATALPMFDCFSDRPDYAYKYKTIKNRIPLNEM
                     NPQRQNLQGADLKFSDQSVKYMVHLIDRGHDDILNRILWRAVKRDLPYPDRFTGESDN
                     ED"
     gene            24136..26586
                     /locus_tag="PKM27_00355"
     CDS             24136..26586
                     /locus_tag="PKM27_00355"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNR05728.1"
                     /translation="MKRMVFYLFASLLLNSCNSVQEGSPGSLVLVQDVEPQPLQAQVI
                     RLVEALDFIGSPLHENDKELLLSLKNKIPDDSSARMIQTILDPYCLAMVEINPEARVK
                     VIRGPAEARIVQAGWTSFIVKVHNAGNVHASLQADSPNALLPFSSRWLWSPTQGNFAD
                     TARQPRSAAETVSRFLELSFYKKPPLLPNLSGLSLEYMILQIYCKDAGKREAELGFNI
                     GQGSQDLGFRNTIPVLFEARPAKKVILKIRDDSNPRGIASFLITDGIERISPDTMHRD
                     QLPDERTWAQMEFGQPGRGRETYVRPKRLSGIYPLPSRRMASMDEFPDFFFQPQIYRF
                     DGEHVYLPSGTFNIRYSRGPEYIEQNQTLVVDGSVDSLVANFTLKRWVDMAALGWYSA
                     DHHIHAAGCSHYDKPEEGVSPEVMWRQILGEDLHMASVLTWGPGWYHQKGNFTGQSSP
                     LSTESHVMRYDVEVSGFPSQHAGHVILLNLKEDDYPGTSTTEQWPTWTYPVLKWAKEQ
                     GAVTGYAHSGWGLEPVEPVTGLPNFVLPKMDGIGANEYIVTVTQGLIDFYSAGDTPPL
                     WELNMWYHTLNCGFRPRISGESDFPCIYDERIGMGRSYFKPSADLNFDQYTEAIRKGR
                     SYVSDGGSHIINFKVNGLEPGLEESELKLSGPATLQIEADLAAYLPEQQDESGRAIAA
                     KRMDEAPYWHIEKARKGNTHKVEAELIFNGKAVDKKEVTADGNWNKLAFQYPVSRSGW
                     LAIRVLPSSHTNPVFVVVGDRPVQARESAEWCRRAVDQCWKMKKDRIQPNERKMAGEI
                     YQKAREQYERIASAADAK"
     gene            complement(26617..26985)
                     /locus_tag="PKM27_00360"
     CDS             complement(26617..26985)
                     /locus_tag="PKM27_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR05729.1"
                     /translation="MHSNPTQFIIEHHFNSFVNNDLEALMSNYTENSKVIMLDIVASG
                     LDEIRRLLTGAINNFPAGEAEIILDTLIVDGDLGYTIWHGETNSVVVPFSTATYLIQD
                     GKIHRHTIGRITQAKTPLDQ"
     gene            27211..30285
                     /locus_tag="PKM27_00365"
     CDS             27211..30285
                     /locus_tag="PKM27_00365"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNR05730.1"
                     /translation="MIFCTGVIHAQWRSPRLTHLGPLQGMTSWTFEVTQDSTGYMYFG
                     TDQGLVRYDGSRFELFAHNPNDTHSIGPGDVQSVMASSGGLIWLGTRLSGLHSFDPLH
                     RRFRHYPYPGIISSGYATVHCILEDGSFLWLGGDNYLLHRFDKTTGQFESFRPAWISP
                     EIENDRFSLTEIFQDRLDPDRLWMALINHNPKYAGKKATWLISFHKGNKTFQRHECWG
                     SPRHQDPDGRIWLTSEGINRYDPASGTCEHFPVFLNGEKQGPEVWIRDLVRHQGSFLL
                     SAPQALLRFQDHGEVREVAYGKELGNMGDLFVDGQKNTWIGRTNGISVLRAQEESIRY
                     FSFSHFGFSGRIYPGRMAYQPDSNKIFVVDHSVDGTGRKIMVLDLDTGISSLFYESPH
                     PIHGIVVDTSGQLWMSSNGAFYTLAVRGNPSPKPKRENSHASPVPWLWNLSLSPGGWI
                     AGTGIHQFWWFKPGEPARVLNLDQLDSAGQGKSVSRLQGLSFTRQDEALLFSNRIFSV
                     DLQSGKITPLLMEDHVNPFPYTDIHSVMQDHRGHIWVSNLAMTAEFKRSGDRLDLVKK
                     YSAGDGLGAAWVHEITVDERGRIWAFAQNGLQVIDPVLKEARSFGVREGLENPYIDPR
                     QVLHLKDGRIVSVNGPGIILFHPDTLWNLYTPRAVPLVIREIRIAGQTLPPDTDLNGI
                     KSLRLKPDQNFLDVQFQALAYPTDFKMTYSYTVDGLHPGWISIGENKIITLPTLSPGD
                     YVLKIKAGEPSSPAPEKELSIHVAAPLIGQAWFRVLSVLCVAGLLAGLFFWRTRRIRL
                     EEAGKALVSRQMAELEMKALRAQMNPHFMFNSLNSIKNYIFRAEPKTAAQYLSNFAHL
                     IRLTLDHSREKRITLKQELDALLLYIELEQMRFENRFELVFDVPETLDLHRLYIPPLL
                     LQPFVENAIWHGIMHKQGKGHLRVGLSKCNGVITCVIEDDGVGRTRAGEIKKLNHSKY
                     QSMGISIMEDRIEIINKLEMLQIRIEILDKLDDLGNPAGTRVNIRIPELSED"
     gene            30302..31057
                     /locus_tag="PKM27_00370"
     CDS             30302..31057
                     /locus_tag="PKM27_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR05731.1"
                     /translation="MTKIKTILVDDEKNSLESLAFEIGEYCPAVEIVAACQDPFRGKE
                     MIERHQPDLIFLDVEMPGMNGFELLQQIPGLSSHVVFITAYDQFAVKAFEFNAVDYLL
                     KPVRISKLISSVQKVLDRVNQKLDRAGLEALVQNLQLKKLPGIENIALPTQEGFKMVR
                     LNEISYLEAESNYTWVVMADRKKYLVTRTLKEMEDQLSQGPYLRVHKSYLVNLNGVDR
                     YVRGQGGYLIMKDQSQVPVARTQKEVLLRSLKL"
     gene            complement(31091..32617)
                     /locus_tag="PKM27_00375"
     CDS             complement(31091..32617)
                     /locus_tag="PKM27_00375"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05732.1"
                     /translation="MRRYYFTILCLACLIFPLTGQVLIQDSVALVALYQSTSGPQWTN
                     KDNWLNGPVSGWYGVTTQGNRVVQLNLWNNGLSGSIPPEIGHLTALHELSLGMSDLEG
                     PIPHSLGRLTELNVLNLRRSGIQGLIPDSLGSCTQLVQLYLGENRLEGGLPSSLAQLK
                     SLRNLHLNKNNLSGPFPEVLTEMPGLLSLDISDNKFSGALPSWLNKLVQLESLAFSNN
                     SFEGSMPKLDSLVHLSSMNFNNNKLSGNLDTLLGHYPEMLYCEGSGNAFTGLLSPAHF
                     NPQKAVILDFSDNLLTELGNFSNWDPNPNFLRVVIINNRLDFDDLAQNAGLPAHKLWW
                     YPQGLIGKDTILVIQAGSKVVLSSPMKNQEIQYTWFRNGNSIPGANGAEYTIESFNPA
                     QAGGYSFRASHPWFLPENPIRGATFHLALPTTAIPELDPSLFKIIYTGSRQTLQVAFH
                     QPFHTKQVSLILPSGQVIKSFKTGGEVITIPMDYLPTGIYFVELKTEKGRGLARIFKR
                     "
     gene            32961..33662
                     /locus_tag="PKM27_00380"
     CDS             32961..33662
                     /locus_tag="PKM27_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05733.1"
                     /translation="MKYSILFIALSFWITCTKDNVQTDQSIDGTFLEERGRKVKISLC
                     HIDENGDFQALSVPQNAVQAHLDHGDYLADADGDGYSAVGACSGSGDDCDDEDPDINP
                     GSGACTGCVCSDLDELNDSNLLFYFNSDNNPCHIYNDPTGVILFFEASGDTMVALAGI
                     EEDGQVIVGLGEVQNGFIQESEHGCKQVVGMDITLEQYETCLAGLRAIIASHPTLPNI
                     CDIDMPPMFIDTNPQ"
     gene            33764..36103
                     /locus_tag="PKM27_00385"
     CDS             33764..36103
                     /locus_tag="PKM27_00385"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HNR05734.1"
                     /translation="MKKFKYSFICVLLCFQPSWQGSFFAQDLCDTVVSAWGMHEFTLE
                     SGISRISFVALGADGGNAAVRNTDIFGNVTTCTSMGGSGARVQATFNIGTGTNEIPAG
                     STIRLIVGQAGENHISDISFGTGYAYGGGGGGSAVLYRAPGSLVWTTLLAAGGGGGAY
                     QGMFANACIDNENGQGGRSGTSGGNGTGDIGPGSGGFSGTSGDGGGASGLISAGGGGA
                     FSDGGGIPCDGFNGEGKAGLPAGGAGGTQETCFLGELFKYGGFGFGGGGAGRGAGGGG
                     GGYAGGGGGGTTGSGGGGGSGVDPRAISQGITAGGNDPASLDGSIAISCSLAPIAICK
                     NVTLDIEEGDTVFVTISDIDDGSYDPGGGALEKVLFSPPFTCDSAGTRFVTLTVYNEE
                     GLSSECMAQVTILEMTPPTIHCQDPIRVDVSGSGLCGAYVPYYMACTDCSSCTILQIE
                     GLPNGAFFPVGTTINSFVAIDASGNRSDTCSFTVTVVDAEMPTAVCLSFVEVSPDTSG
                     NYIPDPALINYGSYDNCGIGSLTVFPSLIPCAHESPKQMVTLIVKDLYGNQNTCSSEL
                     TLLDDDDYDGVGNDCDQCPGGDDQVDNDHDGQADCAVFPGMDHLIDSWKCGNNSNKVL
                     ICHIESLEPFVSTPLCVHPNAVAAHLAHGDFVGPCPGPMEAGSKKDSPSGPIHSEINK
                     GSGTMIASRDWSPVYPNPNSGNFRIRLNGLEISENAPATFSVYSTLGKKIWEEKTFVG
                     ALQTLQVDLHESLPPDGIYLLHIRNGEKSIVKKFQIGRP"
     gene            complement(36174..37391)
                     /locus_tag="PKM27_00390"
     CDS             complement(36174..37391)
                     /locus_tag="PKM27_00390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR05735.1"
                     /translation="MQTPPVTSRLYSTPFLMLCLSHALFAGSFNMILPELPNYLRSMG
                     GAHYIGLILALFTFTAGVSRPFSGKLTDTIGRIPVMYFGVFACIACGVLYPLVHTVAG
                     FLFIRLIHGFSTGFKPTASAAYVADIVPWNRRGEAMGIMGISSSVGSSATPPIGSWMV
                     QAFGINIMFYFSSFLALLSMLILINLPETLGGKESFRFSHLKVKKEDLFDPLALPAAI
                     VMICCYFSYGVLLTLAPDLSDRLQAGNRGVYFTLFTLSSISTRFFAGRIADRIGRVPV
                     LKFSIYIMILSMLVFAHSVSPAWFYWASILFGLGVGIYFPTISAWTIDLGDPAKRGRA
                     LATMYIALEIGIGGGALFAGFLTGAHIDRIPYMFYISGAMCAFAMIYLYRLDRGSLYG
                     LYRQSRNKSIMDK"
     gene            37740..39293
                     /locus_tag="PKM27_00395"
     CDS             37740..39293
                     /locus_tag="PKM27_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNR05736.1"
                     /translation="MKKYLLLTGVILLCIPVLNSQNCNLICLSKVNVSLSPANGCTAN
                     PSPLNFISNPSPTPSPSCAAYQLKISYPYGTSKYNPPTKLDKSHQGYTMLYQVLDSVS
                     RNACWGYLTVNKCNPCMVPTPPVADTLIASPMSGPYPAGSMITLTATATDNVSIAKVE
                     FYDGLILLNTDLTAPYSFKVTTFALSKMYQFKAVVYDNCGARDTSNIVLITTDPPTCT
                     DGIKNGFETGVDCGGGGCSICLPPPAPPACMTPVLLPSGSLDTVRQSSTSSAYYPASK
                     ARDASLYTYSRTNAELRPWWEINLGATYNISSLEIRHRICYGCNNYLIRYNLFISPTP
                     ITTTDINVLKADPLVYKLPYVASSFTLNGVNFLGRYIRIWAEYSSPNSLSLLDVKVRG
                     CQVMMVASSISGKLSDQGSLSVLSTDLQSLTPPEGKPINWQEASDTDPKPSPITVFPN
                     PARDRIFLSIPGGYVQAEALITDILGRKVMRRPLAGTELDISTLAQGHYLLQLQVDGV
                     KTLHRFIRE"
     gene            complement(39742..40536)
                     /locus_tag="PKM27_00400"
     CDS             complement(39742..40536)
                     /locus_tag="PKM27_00400"
                     /inference="COORDINATES: protein motif:HMM:NF023805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HNR05737.1"
                     /translation="MKLLNFTILLLALFILSCQQESQIKTSEEELTGITAEIKAKIIN
                     LGFNPKDSYKTEGGYIVEGDIFLTDQDLVSLPNQRLPILEQYRTTNLVTVNGTRTISV
                     SLDSKLSTISAALDEAIARYNAENLTLKFQRVSKNGYIKIVKSSPSAGYLASAGFPSN
                     GNPYRSVSVNLGALSGQPLGTIASVLAHELGHCIGFRHTDFYDRSISCGGSAVNEGSS
                     SVGAIHIPGTPTDATLAAQSWMLSCIGSGQNRPFNNDDKTALDYLY"
     gene            41093..43516
                     /locus_tag="PKM27_00405"
     CDS             41093..43516
                     /locus_tag="PKM27_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNR05738.1"
                     /translation="MKRGLQLFALYKKNCLLSTILFVLGLQSQDLIAQFRFPPEISRM
                     SFPAFPQTELVINRLDHQVNYIRAAHFYFPDLRGEGIRISVKEYLFDTSDIDLAYAYR
                     FSPHQLPVIQTHSTIMATMIAGRGNSSPSSVGVAPAAGLYSSSFVPALPDTDNYYRAN
                     AISVQNHSYGEDINNVYGPEAAAFDQNAWQNPFLLHIFSSGNRGNAASPSGRYSNLEG
                     FANLTGNFKMSKNSIAVGAIDTADRIETLSSRGPAYDGRLKPELTAFGIEGTSGSAAL
                     TSGLAALLQQAYKKYHDDSLPPSSLIKAALINSALDLGTPGPDFTYGYGKINALQALK
                     TMTGQKYIIGLLKKDQTARHAISIPADVKNLKITLAWLEKPAEPFAPFALRNDLDLRV
                     EEPSTALAHLPYVPNTFPHPDSLTLPASPGEDHLNTVEQVMIADPGPGTYTIILGGGM
                     MDSSQAYSLCYEWEERNRLTWTHPLNIDFLEAGRSQELRWLSTYDPKVEGSLAFSFDS
                     LTWMPILTGLSLSEGKTNWLVPDTNAVVYFRLTADGAEYFTRSVILKKLEMEVGYICE
                     DSVLVSWNKLEGVDSFGIYRMDQGLMRTSGHTPDTFWIFKADPGSPYIAVSTLIAGHE
                     TRLPVLNYTDQGVACFIRQFIGRKIDESNAELTLALGTVFGIKNIRIDRINHRDSLTV
                     LNANLPTQANFTVEAGLKEGSNLFRLRLTLQNQRTVTDEARLYSLGSRIGSVFPNPVR
                     GGQSLNLKNYKTDPVSVTLFDLNGRPLFNTLLRGEGSLDLPGLSPGIYFIGFYENEKL
                     RFREKVVIY"
     gene            43693..45369
                     /gene="ettA"
                     /locus_tag="PKM27_00410"
     CDS             43693..45369
                     /gene="ettA"
                     /locus_tag="PKM27_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNR05739.1"
                     /translation="MAGEKIIFSMEGVNKVYPPNKQVLKNIYLSFYYGAKIGVLGLNG
                     SGKSSLLRIISGKDQNYQGKIIFDKDYKIGHLEQEPVLDPEKTVRQTVEEGVGALVAL
                     MKEYEEVCNKLAEPLEDNEMNRLIEKQAELMDRLDQENAWELDHKLNTAMEALRCPEE
                     NQIIKVLSGGEKRRVALCRLLLSNPDILLLDEPTNHLDAESVLWLEQYLQNFPGTVIA
                     VTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKAKRLEMEEKQETKRKKTLERE
                     LEWVRMSPKARQSKGKARLNAYDKLLSEDIKEKEATLELYIPPGPRLGDVVIEAQGLS
                     KAFGDRVLFENLSFSIPKNGVVGIIGPNGVGKSTLFKIIMGQEKPDTGKVTIGDTVHL
                     SYVDQTHKDLNPEKTIYDVISGGTDIILVGKTEINARSYLARFNFTGGDQQKKVGVLS
                     GGERNRLHLAMTLKDGGNVLLLDEPTNDIDVNTLRALEEAIEHFAGAVLVISHDRWFI
                     DRLATHILSFEDDGQVVYFEGNFSDYEAKKKERLGDLTPSRPRFKNVINR"
     gene            complement(45438..46217)
                     /locus_tag="PKM27_00415"
     CDS             complement(45438..46217)
                     /locus_tag="PKM27_00415"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR05740.1"
                     /translation="MKYTSLALLLGLILFACKGKEKESTPAMEPSTAPTATNPNRVVL
                     TPEETQNGWRPLFDGISTTGWHKYGGGAPGPAWKTVDGTLMLDASNKADWQTQGGGDI
                     VTDEEFENFHLKLEWKIDTCGNSGIIFYVNEDTAKYPYVWHTGPEMQVLDNACHRDAK
                     IKKHRAGDLYDLISCSKEPVTHALEWNQAEILSQNGAIDLFLNGEKVVSTRVGDPAWK
                     KLVAGSKFKDMPGFGAFTKGKISLQDHGNTVWFRNIKIKPL"
     gene            46517..50047
                     /locus_tag="PKM27_00420"
     CDS             46517..50047
                     /locus_tag="PKM27_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HNR05741.1"
                     /translation="MIDKLDQFIRKYYINQIIRGVLFTTGSVLLLFLAYNLMEHEFYF
                     SKGVRKGLFYSFNLFSAGALAYWVGVPLMHYFKLGQIISYEQAARIIGDHFSDVKDKL
                     LNILQLRSQLQSLEQSQLIEASIQQKSAQIQIVPFKNAIDLNKNRKYLRFALPPLLLL
                     LVLLLAAPSVIRDSSRRIIRNNEEFEKAAPFHFKLKNENPSVVQFDNYTLDLEVDGMV
                     LPAEVFIEVDQYPYRMTRVDANHYTYTFNNVQKNTGFTMFSGEVKSKGYELAVLKKPI
                     LSNFTIRMDYPSYIGRKAESLENIGDLLVPEGTRLAWQIDAENTDKVAMAFGSGTVQE
                     ISRNGERYFTHRHRAMQDQPYKIFISNQWVPVPDSILYQLRVVADQYPSIQLEQFVDS
                     LDKRLIYFAGNGSDDYGLSKLTFNYSVIHPDGKQVNAVPVAIPISNSNKTTYQYEWDS
                     RNIELKPGDQLSYYFELFDNDGIHGAKSVKSNVLSLRMPSEEELENQNSKNNDAIRDE
                     LRQALEQSRSLQQQMDQLREKMIQEKKPNWQLKKELEKLLEKQKEIQKKIEEARKKFE
                     ENLKNQELFEETSEKILEKQEQIEKMFEQLQDPKLQELMEKIQELLQQMEKDQTLPMM
                     EEMNIENQELEKELDRMMELFKTLEVEHQMQMAIDKLREMAKEEEQLSEETRMKAEEE
                     KQPQNQENPNKDPQDNKGEQKNSDQKNKQDQPVKEDPQNKSNDENKAGQDNQEKDQKG
                     EQEKQGADQKQPESKSQEELQKQQEELNEKMEKLQEEMKELEKKNEDLQRPKQMGEPQ
                     KKMEDIKQEMQNSSKQMQQKQNNKASKSQKNAAQKMKDMAQSMENQMQQEEQEQAQED
                     VKALRQLLENLVTMSFDQEGLIKEVNKTMLNTPRYTELLQQQFKIKEDFSLIEDSLQA
                     LSKRVFQLEAFVTEKVNEIKTQMGSALDELEERNKAKAGDHQQRTMKNVNDLALMLSE
                     SMENMQAQASGAACANPKKSGKGEGQKPMDKITEGQKGMKEQLEQMKKAMEEGKGQGS
                     KEFAKAAARQAALREALRELQKEKQQRGENTQELQKAIEGMDKTETELVNKKLTNEMM
                     QRQNEILTRLLEAENAERERKQDEKRKSESAQELERKLPPSMQEYLKKREAEIDLYKT
                     VSPDVKPYYRSLIEEYMKSLRTEHGNTINK"
     gene            50025..50462
                     /locus_tag="PKM27_00425"
     CDS             50025..50462
                     /locus_tag="PKM27_00425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR05742.1"
                     /translation="MEIQSISEPGTRPLMNTYWRFPSTPGCITDIEKLVEYLADKYQI
                     SAEKYPNILISLTEAVNNAVIHGNHCDECKCVHVEVEVHPQSLTLTVRDEGCGFDPAN
                     VEDPTLPENIAKAGGRGIFLIHQLCDRVRFDANGTTVKMSFDF"
     gene            50496..50903
                     /gene="ybeY"
                     /locus_tag="PKM27_00430"
     CDS             50496..50903
                     /gene="ybeY"
                     /locus_tag="PKM27_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNR05743.1"
                     /translation="MIRFHYRYPAMRVREAGRLKSWLAGVAKNEGKSVSSMDYIFVDD
                     PVLLEINRQSLHHDYFTDIITFPYQSKPIIGEIYISVDRIREHAAEYGVSFSQELHRV
                     MVHGLLHLCGYSDKSGKEKKRMTEKEDFYLDQV"
     gene            complement(50972..52501)
                     /locus_tag="PKM27_00435"
     CDS             complement(50972..52501)
                     /locus_tag="PKM27_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HNR05744.1"
                     /translation="MKLKHYFFAFLLTGSHYAAGTDYNILDYGAVPGQWSTRAIQKTI
                     DTCHAKGGGRVTVPAGTFITGSLFLKSHVELHLSPGSVLESSLNLTDFRVDNRTYGMI
                     YCEDATEVAITGSGTIHARGIDFYERDKNHLFPEFDRSRIRQRENYMPEGRFFTDGPL
                     KRKPRPGMSIVFFHCTGVRLSGITVKDTPIWAIRFGYCDNVLVDGLTIRNNPLIPNSD
                     GIHCTTSSNIRIENCDIVAGDDAIIMTGFDRIENVPDYSMAEQESHRYGNKSPYAENI
                     AVSNCRLKSSSAGIRIGYGQHPIRRGVFSNIIIQDSHRGIGIFAHDKSNIEDLIFTNI
                     IIETRLYNGQWWGHGEPIHLSCISRFEGVRAGQIKNVQFNQINATGEQGILVYGLEES
                     RLENIRFNQVQLRMRKGKETMDYGGNFDLRPATPISMQLFEHEIPGLYAQYVNQLLIR
                     DFEVQWADDLPSFFTHGIQCENITGLSIENFNGTANPNAAGSQKIFLKNTSPPVGTER
                     H"
     gene            complement(52708..53706)
                     /locus_tag="PKM27_00440"
     CDS             complement(52708..53706)
                     /locus_tag="PKM27_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05745.1"
                     /translation="MGIDILGFGVCAGVVYFAGKKLSYFGDKLAEFTGLGRAWIGMIL
                     MATVTSLPELMSGVSASVLIESADLAVGDILGSCAFNLGILSLLDVFTPKDRPLFTSL
                     SQSHILAASFGMILIVLAGLGLFLEDDLILTPSIGMTSIGFLLVYFFSIRTIYKYQRT
                     FQKPGEQHSAAVGDLNPKQVLWRFSVYALIIVLAALSLPYFADRIAGHTGLADSFVGT
                     LFLAISTSLPEIAVSIAAARMGSLDLAVGNLFGSNLFNIFILFLDDLLYTKGHLLKDA
                     SDVNLVSVFFIILMALVSVIGLLFPITRKKIVLAWDTLVIFLLYALNIFLLFRLLH"
     gene            complement(53935..55329)
                     /locus_tag="PKM27_00445"
     CDS             complement(53935..55329)
                     /locus_tag="PKM27_00445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNR05746.1"
                     /translation="MKKIVRLILVVGVFVFLVSCEREQIDIPVSEQALAQSEPGQFVP
                     NQLLVKFKPIKKSGARRTSEALKQIDGEIIERISTPAMKTANINPADGEAELFLVNSP
                     LGTGEAIQRLQRLPEIEYAEPNWIYNHFASSNDTYYSNGSLWGMYGAATSPANQYGSA
                     AASAWANNHTGSSTVYIGIIDEGYMYTHEDLAANAGKNPGEIAGNGVDDDGNGYKDDV
                     YGWDFDGNNSSVFDGTTDDHGTHVAGTIGAAGANNKGVAGVCWNVRLLSAKFLGRRGG
                     TTANAIKAIDYFTDLKTRHGLNLVATNNSWGGGGFSQGLQSAIERANQAGILFVAAAG
                     NSGSNNDSSPSYPASYPNANIIAVASITNTGALSSFSQYGANSVDLGAPGSGIWSSIP
                     KSSKGAVISGYASYSGTSMATPHVTGAAALYAASNPGASAAQIKSAILNSTTATASLS
                     GKCLTGGRLNVGGF"
     gene            55511..56599
                     /locus_tag="PKM27_00450"
     CDS             55511..56599
                     /locus_tag="PKM27_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNR05747.1"
                     /translation="MKFPLSLLFFLSLLSACKNSSPETVSSGTGSDTTSVKAEPKAIS
                     DPLITAIYTADPSAHLFDGKIYIYPSHDYEAGVPQDDLGSHFAMKDYHILSMDSIGGA
                     VTDHGVALALGDIPWAGRQLWAPDAAFTNGKYYLYFPAKDKQDIFRIGVATSTSPSGP
                     FKAEASPIKGSYSIDPAVFKDEDGKFYMYFGGIWGGQLQRWQTGKYDTTGVEPNDERP
                     AMGPRVARLQNNMLQFAEPVKEVQIVDSAGNAIQGKDHDRRFFEAAWMHKYQGKYYLT
                     YSTGDTHFICYATGDNPYGPFTYRGIVLHPVIGWTNHHSIIEIGGKWYLFYHDSSLSG
                     GQTHLRCIKVTELHYNADGSIQPVTAYL"
     gene            complement(57101..59023)
                     /locus_tag="PKM27_00455"
     CDS             complement(57101..59023)
                     /locus_tag="PKM27_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05748.1"
                     /translation="MYLFLHLLISTLFRLQPSYLSPQTIPSQNRCRSIQFEVEPPTLV
                     CAGFEWSIAGDKNRNASVEVSYRKKGDQDWKPALPLLRIGGERIYGHEQRWEYLVPDK
                     FAGSIFNLQPATWYECRFRLSDPDGIEGETEHLVNIQTRAEPQPFPQGQVYHVYPPGH
                     TGPKEQPAFTGLNEAYYGIGNTGDWWNVPEARVRPGDIILVHAGLYKGNRLRYADALA
                     LDFHGAYVFTQKGTADRPITIKAAGDGEVIFDGDGAYHLFDVMAADYHYFEGLTIRNT
                     DIAFYAGLKKVMGCSGLTVKNCRIHDVGIGVVTYSADSRDFYIADNILTGRHDPDTLV
                     GWYGLENPAPLTSYYAIKVYGQGHVVCHNTISFFHDGVCIDTHGLPEDGKACVSIDIY
                     RNDIFNMSDDFIEADGGVHNIRVFENRGFNSYHSALSAQPVFGGPVYFIRNICYHIPG
                     TALKYTVRPSGIYTLHNTFIAEAAITNFSNGHFRNNLFIGPDDTRPSLQATNYTTYSS
                     LDYNFYREKPAGPGRYRWRSPLHDSLNQADDKELGMRVFNTLEEFRQHTGMEKHGMET
                     SPDIFMQVPLPDPKQRGRIYPKDGYDFQLKAEAEVIDKGVVLPNINDGYSGKGPDPGA
                     LEYGQAGPGYGPRSRE"
     gene            59202..61400
                     /locus_tag="PKM27_00460"
     CDS             59202..61400
                     /locus_tag="PKM27_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR05749.1"
                     /translation="MNSAFLRKATGIVLGHLEDESFSGNELAEKLNLSREQTHRKIKQ
                     FTDLSTGKFIRSIRILKACCYLLEGNLSVSEIGYKVGFGDPSYFNKCFREETGISPGE
                     IKKSGDLNPLAKTPLLSFYQVPEIKEQLRNHGMVFEFPQQESINRGRPGIWLSVVPIS
                     IFLLLALFFMFGKKRSTIKIDPGENNRIAVLPFINHTGDSSLEGVGDMASSWISGQLA
                     ELKMIQTVPYFTVKQYQSYIGILPGDPLGRPTFRDLVAARYFVNGNYYLKDDRVYFDA
                     SFVDAVSLQTIYHLPTLHGAVDSVMNTIEDLRLTFAGLITNLDEVKLGKRKPPNYEAY
                     HAFLTGLHQMTVGLATPESRLYLEKAAALEPDFVMPRIFLVWFYQGKKLDTLLQEIAS
                     IPNITTYERDVYEMLYHLLNHQYKESLRIALQNLDKYPGDYFFNLFAGHNAKSQFMPR
                     LTLKVLDQIKDPFPNNEWGMWHYYKVWNYTESLAMLGQYQEALDYLQSIPLKNYSLAI
                     PQLFIQVQVRLGKTSRELEALIEDIAGEKMKYLAGRFDVDEQKVLAEYYTAAAYEYQL
                     ANHSTEARYFADKAVSHYALIPGQKAYKYDMLDALYLSGDYQKTKLRLEKELKKDPAN
                     DELLIYLAQVEAAMGKEGDALNLFAKYDSLPRMYWRRHEFPYHKDYLKARIYALLGYR
                     EQAFTLLQNALYQGQLCHNWDFDRDVFLQSLLDYAPFREMVRPVDGPIPQ"
     gene            61509..61988
                     /locus_tag="PKM27_00465"
     CDS             61509..61988
                     /locus_tag="PKM27_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR05750.1"
                     /translation="MKKIMNAFVVFLFLVVSGKSPAQSAASREKAGKPEPHREQHIAI
                     EDFPKNMAINHLNGWGGMTVAINEMPAGTDLSPLLEGLKNNSCQVPHWGYIIKGSLRL
                     KYDDGKEVVLKAGDVFYMPPGHTGSVVEDLKLLDFSPEKEMKGLISHIEKKIASPKQ"
     gene            complement(62132..62350)
                     /locus_tag="PKM27_00470"
     CDS             complement(62132..62350)
                     /locus_tag="PKM27_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05751.1"
                     /translation="MPLFHLFPYRITDTPPNRFSKGRQSSPKPLLFHSIFRWSPPTLV
                     CAGFEWSIAGDKNRNASVELSYWKKGGR"
     gene            62521..63360
                     /locus_tag="PKM27_00475"
     CDS             62521..63360
                     /locus_tag="PKM27_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05752.1"
                     /translation="MSKQFSFIILISAFLIFCMFRPFMPGRYDQLAVPISFMVQIFSI
                     TGLSWVPLGALWLISVRDESGFRKWGNRFLIYALILSGLLALLVSVAPFSQNNTSFAV
                     LFLAFVSLVLGMAYGKIKNSAPLDRSFHRAILIYLILIPLLVFTSRFLFMERAVQFSR
                     DLAIRNSAGLIQKIEDYFKSHGQYPVSLQALHSDIVPGVVGIPGYFYEPNGQAYNLYF
                     KPLSDELDADEIVMYNKQDEHRFSAHDLDLLEYSGEALALRRGDRRRLRLSAPHWISI
                     KFE"
     gene            63529..66282
                     /locus_tag="PKM27_00480"
     CDS             63529..66282
                     /locus_tag="PKM27_00480"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNR05753.1"
                     /translation="MKKFFVLNLLLLTASLTQAKITPTHLRCEYLENPQTIDVPKPRL
                     SWINTAGPGERGQFQTAWEIRVASSKEKLLVNQADLWSSGKRKSGQSTLVPYQGRPLT
                     SRQDCWWQVRTWDKNGKVSAWSETAFWSMGLLEPHEWKALWIGAPWQGEEAMPKPRFR
                     AAGDTSALPYSPPPAPYLRKSFKLSGKEVVSARAYVTGLGFFEFYVNGKKVSEDVLVP
                     NVTAYGKRPGLEKNYISIPDTFDYRVMYLSYDIKDLLQTGDNVLGAILGNGFYDAPMH
                     WTHSYGSPRFLGQVHITYQDGTEELIVSDQSWKASKSPIVMDLVYDGEHYDARLDQAG
                     WSSPGFEDGHWEPAALRKKPEGYMKAHMSPTDRVMERLKPVKIEALESGKYRVDFGQE
                     ISGWLRLIDVEGEPGRKIDIKYLSESYMGTNSYTLKGGGPESYAARFTWFVFREVVIS
                     NWPGLLRPENLVAEAVYSDVKTTGKFETSSNLFNDIHKIWWRSQTDNMHGGIASDCPH
                     RERSPYTGDGQVACVTVMHHFDAGAFYTKWIQDILGAQNRQTGYVPNCGPWQPGCGGG
                     VAWGAAINIMPWEFYLHYGDLDMLKNNYEGMKGYIKYMLTWTNPEGIMHSQAPDPAKP
                     NQWLNLGDWAQPFELPPSDLVHTFYLWRCADFTARTAKVLGDQSEFLHYQSLADKTRA
                     AFRNKYYDKVKGTYGPYGGNIFALKMGLPPDQYHRVLAALKADILARGGHLDTGIFGT
                     QFFFEVLSENGLHDLAYEAMNKTTQPSYGWWLSQGATTSWEHWNGRGSRNHPMFGGGL
                     VWFYRVLAGMNADASKPGYRHLVFKPQPAGDLSFVSYSNQTPYGLAGVNWKKHEGSFL
                     LNVDVPVGSTATIYVPASGPEAVTEGGKKIKNNKTLRFERMDGGHAVFTVGSGRYSFE
                     AKR"
     gene            66396..66824
                     /locus_tag="PKM27_00485"
     CDS             66396..66824
                     /locus_tag="PKM27_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNR05754.1"
                     /translation="MSEIKTKQTGADVYEFIRTFADTEQKRQDSLALIQMMREVTGYE
                     PKMWGPSIIGFGKYHYKSERSRQEGDWPLVGFSPRKAAISLYVYTGSPEQAHLLEGLG
                     KFKMGKACIYIKKLSDIHPGVLKNLMKETIHFLQARFGKG"
     gene            67206..68555
                     /locus_tag="PKM27_00490"
     CDS             67206..68555
                     /locus_tag="PKM27_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR05755.1"
                     /translation="MKKLKGPRPALPAGPGWARFIVYLTFLLKIEPVLAQTPNLVYIM
                     TDDMGYGDLSGYGNKKFATPNLDKLALQGVKFLNAYAAAPVCTPTRTALMTGRYPAKT
                     PVGLMEPLRSPTLDSAYGLTGDIPTLGRLIKARGYETALIGKWHLGFMPDQRPGKNGF
                     DYFFGILSGGADYVSHKGTNGTKAHDLYENDSLVYTQGYLTDLITQKSVAFIKQKHLK
                     PFFLTITFNAPHWPWQGPADPAYPDSMAFTAGGSPSIYAAMMKSLDEGIGKIMQALDE
                     EQLSRQTLVIFTNDNGGEKYSDQGGLSNRKFTLWEGGIRVPAFVRWPGKIEAGMSTPQ
                     VAITMDWTATLLKAAGAKADKNFPLDGIDLMPVLTGKKKEMHRTLFWRTFQRSKHKAV
                     RDGHWKYLQDEEGEYLFDLTADQEEKQDLRKEHPERFERMKNKMNRWEKTVLPPIPL"
     gene            68608..70473
                     /gene="mnmG"
                     /locus_tag="PKM27_00495"
     CDS             68608..70473
                     /gene="mnmG"
                     /locus_tag="PKM27_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNR05756.1"
                     /translation="MFPPYDVIVVGAGHAGCEAAVAAANMGSRVLLLTMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDALGGYSGIVTDHTMVQFRMLNRSKGPAMWSPRAQSDRNLF
                     ARKWRTLLEAHPNIDFWQDMGSGILVKEGRVTGVRTAMGLEIESKAVVLTNGTFLNGI
                     IHIGEKQYGGGRAGEKAAQGITEQLVSLGFESGRMKTGTPPRIDGRSLHYERMELQAG
                     DEPAGKFSFTDTPRLEKQLPCHITYTNGEVHEILKTGFDKSPMFAGRIQGLGPRYCPS
                     IEDKINRFADKERHQLFVEPEGWDTCEVYINGFSSSLPEDIQYKALKKIEGFEEVKMF
                     RPGYAIEYDYFPPTQLSTSLETHLIKNLFFAGQINGTTGYEEAACQGLMAGINAHLAI
                     HEEEPFVLRRSEAYIGVLVDDLVNKGTNEPYRMFTSRAEYRMLLRQDNADLRLTPLAF
                     KLGVGGLEARMDAVKTKQGGAAQIEAFVKSASVSPDQMNGYLESIGSATLSQKVKLLT
                     ILLRPEISLPALGEKIPVIGDFLSAFPEDSVELAEIDIKYEGYLQRERDLVDKMNRLE
                     EMKLLDNFNYKAITSLSAEAREKLSRHKPRTIGQASRISGITPSDISILLVHCGR"
     gene            complement(70556..71440)
                     /locus_tag="PKM27_00500"
     CDS             complement(70556..71440)
                     /locus_tag="PKM27_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR05757.1"
                     /translation="MAEMFKEITPLTQYDCFLVFERHKKDFDFPIHTHDEIELNFIHN
                     GAGVLRVVGDHSEEIGDLELVLVGSRLPHCWMTHNYKYNKEKGEMFEITIQFHKDLLD
                     EKFLRRNQLIFIKSLLEKAGRGISFPRETIAQLEPRIRKLSKLSGFDSVLELFSILHD
                     LSLTRGIQLLSNESFADEKPTFINRRVELVYDYIRSNYHKSITLGEISKMVGMTEVSF
                     SRFFKKTTCKTFVDSINDIRLGNASRMLMDSSYSISEIAFKCGFKNQSYFNRLFKEKK
                     GETPKEFRERYAGTRMFV"
     gene            complement(71847..72515)
                     /locus_tag="PKM27_00505"
     CDS             complement(71847..72515)
                     /locus_tag="PKM27_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNR05758.1"
                     /translation="MPLRPKSGRLVSAGALLFFLFIHSCGLKPPTEKGAFKATDLVEL
                     IRIDSTFKLDIRYATPNNLAGRPVYTEARAFLQRDAALALSRVNQALRSQGYGLLIFD
                     GYRPWSVTKLFWDITPKEKKIFVADPRKGSKHNRGCAIDLSLFDLRTGLEIEMPGGYD
                     EMTERSYPDYRGGTESQRTMRDLLRRHMEAEGFTVYESEWWHFDYKDWPMYRIGNIPF
                     EQIR"
     gene            complement(72562..73104)
                     /locus_tag="PKM27_00510"
     CDS             complement(72562..73104)
                     /locus_tag="PKM27_00510"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNR05759.1"
                     /translation="MNRALHRDLGYFCASLILAYCISGIALNHVDDWNPDFIINRDTV
                     YFDQSMSRENIQRDDILKLAAMVGMPSFKVYDFPTPDQLKIYFDRATLHVYFHEKMGL
                     YEGISRRPVFYQTNVLHRNSVRNWKWASDVFAALLILINLTGLIMLKGKYGIGGRGKW
                     LILLGLIPPAVALMMQVSQA"
     gene            complement(73116..74063)
                     /locus_tag="PKM27_00515"
     CDS             complement(73116..74063)
                     /locus_tag="PKM27_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNR05760.1"
                     /translation="MKRISRSRQLAVLFLLATMLLSCKRGGETLALNAGEGKNIGIVL
                     VNHGSRSATWRAALHDLEAKVKDSLLLVPGVSVVKTAFMEYTEPSLATQLKACDSAGV
                     DDIIVVPVFLTVSSHSFDDIPTIIGQKADPQSMETLKLEKIERYTPRAKTHITPLLDF
                     PGVLKQNTTRRARVLSANPAEEGLVLIGYGDAQYNREWSALFEDIAGFVKQETGIGAY
                     AYGWCGHLVHYNPDSTSAAIERVLKEKSKAVVIPVLLAHDEMFQVRIIGDGITKIQDH
                     EQRIAYRADAILPDPNVETWIKNISREFAANIQPATAER"
     gene            complement(74156..75190)
                     /locus_tag="PKM27_00520"
     CDS             complement(74156..75190)
                     /locus_tag="PKM27_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNR05761.1"
                     /translation="MKSVFVFLLVFLVPFISSAQAPVEVLDSGFVYELDAAPTPECHA
                     STLASTPQGPVIAWFGGTHEKNRDVGIWFSRMHKGQWTKPREVANGMISPAERYPCWN
                     PVLYQQKKGPLFLFYKVGPSPSEWWGMLIKSMDGGKNWTKPERIPSPFLGPVKNKPIL
                     FDSVLLCPSSTEHEGWNIHMETFNLKTGSWSRFQAIHPNSGFEVIQPTLLMHPGGQLQ
                     LLNRSKQGIIVETWSGDGGRTWSPFGASILPNPNSGIDGLSLKDKMHLLVYNHSGIPK
                     GKWGGNRYPLNLAASRDGIHWKTIMILEDQAGEFSYPAIIQSEDGLVHIAYTFNRKTI
                     KYLRVKVGGG"
     gene            complement(75187..76584)
                     /locus_tag="PKM27_00525"
     CDS             complement(75187..76584)
                     /locus_tag="PKM27_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNR05762.1"
                     /translation="MSAYTSGEKKIPAWVAGLSIFATYVSSISFLALPGKTYQSNWNA
                     FVFSLSIPLAAWIAVRFFVPLYRKVGSVSAYQYLEGRFGPWARVYAAGCYLLTQWMRA
                     GAILLLLAIPLQTAFGWNQAWIILVTGLAVTGYSMMGGIRAVIWTDALQALILISGAL
                     IVAFLLVWRIPGGISEVVRIGIEWDKFDPGSFSAGWSESTFWVVLVYGLFINLQNFGI
                     DQNYIQRYMVTGTEAGAKKSTWMGSMLYLPVSFLFCFIGTALFAYVQVHPDFLPPGLK
                     NPGAADQVFPYFIVHDLPAGLTGLLVAALFAAGMSTVSTSINSSATILLTDFYKKFIR
                     AEPGNKKEMQVLYLSSLLVGLAGISIALSLQGVTSILDAWWALASVFSGGMLGLFLLG
                     FISERTQSRHALAGVIAGVILIGWLSLSPLLLKGGPWSGLSNPFHPNLTVVFGTTIIF
                     LTGFLLSYLAAKLRK"
     gene            complement(76748..79396)
                     /locus_tag="PKM27_00530"
     CDS             complement(76748..79396)
                     /locus_tag="PKM27_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNR05763.1"
                     /translation="MKPLAFYIFLLLLLQACSSSRPSAKSVVRPAEEDSLLTEEMEFR
                     QLDTMVVSPEMDENLSYSEDEEETVPYRSSHPRRWDLLHMSLQLKFDWENEQVHGKAS
                     LRLKPLFYPTDSLELDAKSFDIHTISAGGQSKNLVYRYDGEILKISLGKKYSRKDTLD
                     LFLEYRGNPSRNQTTGSAAITDDNGLYFIDPRGTDPNLPTQLWSQGETEYNSRWFPTI
                     DKPNERCTGEISLTVPDSMLTLSNGLLVKSTRNTDHTRTDTWVMDQPIAPYLYMIAAG
                     PFAKVTESWQGIPVEYYVDKPYGAYAKEIFNHTPEMLEFFSKKLDLKYPWKKFSQVVV
                     RKYVSGAMENVTAVVFGDFIQKTHRELIDDPNDNIVAHELFHHWFGDYVTCESWANLT
                     LNEGFANYAEYLWSEHKYGKYEADRHRENELMGYLSSVSFGQAHDLIDYHYADKENMF
                     DAHSYNKGGLVLHMLRHYVGEEAFWTTLNLYLRRHAFQSVEVHDLRLAFEETTGEDLN
                     WFFNQWYLKPGHPTLSYTHTFDASTKTVLLQVRQTQEKEFPGVFRLPLDADLHFANGE
                     IVRKRLWLDHRNQQFELEVDSKPSLIVLDPDRILLTEWDEPELTANEYKIQWKSCNNL
                     QLKLNALNALTEERDYIQIWREAIQEPYWYFRLRALDYPPSLKPEDINKLKWMALRDP
                     HSQVRAAAIRILGEGTGINSLEATLDNILEADSAYVVLGEALKALARINPVKAGLAVK
                     NLQQEKNPYIQEAIAEVLAAENNPASLDYFDQKLADDPSLAANLLDKYLAILKDVPAE
                     VQFSKAGFLFEQSINPNLDKTSKFYFVRALFEVKEMILTRAVHEKDPAARAVLENRVK
                     EVKSWIQEIKEKETDVQLKSILGQFN"
     gene            complement(79425..79853)
                     /locus_tag="PKM27_00535"
     CDS             complement(79425..79853)
                     /locus_tag="PKM27_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05764.1"
                     /translation="MLRKSLFALITVQFLTAFFHSMSFLGKPRATNETEEKLIDLFTN
                     YKQDLGAGFSRSTFDLFNGLSTSFTLICLLGGWINLFFLRKRLDASLWRGLLFIEMVV
                     FGALFLFMILFTFLPPIVLTGLIFISSTMAWHYSTKDTLS"
     gene            complement(79909..80508)
                     /locus_tag="PKM27_00540"
     CDS             complement(79909..80508)
                     /locus_tag="PKM27_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR05765.1"
                     /translation="MKNRLTLIFTICTLAALNAQTKAQLFNLKNLDGWIVYGTEKWYV
                     ENQELICESGPDKGYGYLGTAGTYKNFDLELEFKQEANGNSGVFFHSSFEGTKVTGWQ
                     AEVAPPGHSTGGIYESYGRGWLIKPTKGKDTKYLKMGEWNHMRIRVKDDQVTTWLNGH
                     KMIRLKDDKIGSRSGQIALQIHDGGGIKVRWRNLFVKTL"
     gene            complement(80539..81831)
                     /locus_tag="PKM27_00545"
     CDS             complement(80539..81831)
                     /locus_tag="PKM27_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05766.1"
                     /translation="MKRTIYILFALSLFTGLSCKESKPKTEVPVIRNPNDLRKWIVDN
                     TVQDYERNTFGYDLRFIRQRKTPILLYDENFKSMVLLAPEYQGRVLTSTADGLQGYSF
                     GWLNYDLIASMEKLPHMNAYGGEDRFWLGPEGGQFSLYFKPGEPFVYDHWQVPYGFDI
                     EPFEIVSIESNEVAFFKKLELKNYSNTPMEIVVSRIVSLLSPAAFLYTHGIQQAEDIK
                     AVGFQSRNVIRNAGQNEWNKQTGMPSIWILGMFKPSPTATVVIPYKEGPESSMGALVT
                     DDYFGKVDADRLKTGDGVFYFKADGRKRSKIGVNAFRAKPLCGSFDEERNILTIVEYS
                     LPDTAGDYVNSLWKIQEAPFSGDVVNAYNDGPLENGTQMGPFYEIESSSPAASLKPGE
                     SLEHSHRTLHFQGKESSLDEIARKLLGVGLKDIRNAFE"
     gene            complement(81869..82846)
                     /locus_tag="PKM27_00550"
     CDS             complement(81869..82846)
                     /locus_tag="PKM27_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05767.1"
                     /translation="MRIPAILFLFVPSFIIFSCKQDPRHAETESALTGKAVEISGTAG
                     WEADDRFIVSQPSNIIQYNGFSYVFYVKEPVNSPIAGSGYGGTIHYAFSRDQGHTWSD
                     QGLLINVGAPDQFDGGGVSKPAAIRVGDERFYYLYYVGARLGFTNQDGSPENKTGIGL
                     AKLLFNEDGPIRLSIKLNSAAPVLEASEEGSGRFDAFRVDDPNPINLNGQVWLYYTGL
                     DKWGGTPRTGLVVSANINESHIKQNNVRALLDGSPSLIQRQGPGVIAVFTDTQNAWFA
                     ADGLKFVKLKQAFPKQVEGARGNSEKDALTWGLAPPPPGNSGFIRWEIK"
     gene            83211..84131
                     /locus_tag="PKM27_00555"
     CDS             83211..84131
                     /locus_tag="PKM27_00555"
                     /inference="COORDINATES: protein motif:HMM:NF025123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HNR05768.1"
                     /translation="MWHESLALRSLIWPHRGRLCLTYILFSLEMTGSLLRPFFIGRAI
                     NDLIDHQSRGLLELVIVHLAWVVISYLRHRLDTRTYSAIYTAIMTSILSRIYPGKEVS
                     KWSARSGLAKDLVDFLEYDVYFILEAAYNIFGSLILLSIYRSPVVWLCLAALVPVVLI
                     GFWYGRKMRSLQKAKHDELESQVDILTTGNISRIKDHYHRLQRWQIRISDQEALNFGL
                     MEIIVISVITLALLVSTGPSADKIHAGDLVGIYYYILKFLNGLDTIPYTLQRWSMLGD
                     ILRRLQPGPVSGIKTIEQEEVVIKEEKLVA"
     gene            complement(84134..84790)
                     /locus_tag="PKM27_00560"
     CDS             complement(84134..84790)
                     /locus_tag="PKM27_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNR05769.1"
                     /translation="MGANIGDTTVPMALAAGPEGTTLGFDPNPFVFKILRQNALLNRD
                     KTNIHAHPFAITVEEEEFYFISSEASFANGGISKTKESIHGKYIQPGKIKGVNLKKFL
                     EQQYPGQLNKLSFIKIDTEGYDKEIIRSISDLIEAFRPVLVAEVFKKSSPQEKSELFD
                     LIHDHHYDLFFFEDFDVRARVVPVKTREELSNFRKTINVYALPNPYSRSMERSGPKDA
                     "
     gene            complement(85098..86090)
                     /locus_tag="PKM27_00565"
     CDS             complement(85098..86090)
                     /locus_tag="PKM27_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNR05770.1"
                     /translation="MKKVLIIRFSALGDVVLTSLLVRCLKTQIGPEIHYLTKPPFAGL
                     LNANPWIDRVWTLEQGWSGILPLLKAEKFDLIIDLHKNLRSLRVKLALWRVPSTSYQK
                     GNLSKWLSVYFKINRFSKDHIAIRYLNSLRKYKVFDDGKGLDFFIPDADRVDPHGHFG
                     IKGPYLALVIGAAHFTKRPPVVKWQELISLVNGKYPIVLVGGKDEQAAADRLVTGGVY
                     NACGTFTIQQSASILQQALAVITPDTGMMHIAAALQKPIRSIWGSTLAEFGFWPFYGN
                     GREDQNLSFEIKGLSCRPCARFGRKACPKGHFNCMMQQNMSWVADSVLTRNSQD"
     gene            complement(86122..>89280)
                     /locus_tag="PKM27_00570"
     CDS             complement(86122..>89280)
                     /locus_tag="PKM27_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNR05771.1"
                     /translation="IMRFFIVFIFCSGMLTGQVPFKSIPFTSRTEKNQVLQPMKKLPL
                     PVSRHKQAPPAWNEFRPLKFTPGKTAARPTAIDYIHKDHHSFWANSSKKTGFNQRNAQ
                     SSVSAFLEPLKEAMKIKTVDQEFQIVSRETDEVNMQHIRVRQVYQNIPVYGGELVIHG
                     FKDQLSQVNGTVYPTPLSLNTHPRIQAAAALDIVKKDLEAEGRLHAVSDQLLAILNRP
                     LSTAVLTIFHDSADRPHLTYEISYVANGLEIWKYIVDAEQGQIFKKYKNTCSLLPHDH
                     CKGHPEAGLNPVTDTNTGNPSFEFAGKTSTPAMDLNGSLLSLNTYEEGGRYYLLDASR
                     SMFKSIGTDTEEPSGVIWTFDGKNNSPSNPNFSANLINGSTNTGWISPTAASAHNNAG
                     LAYQYFLSKNNRLSINGQGGNIVSMINITEEDGSQMDNAFWNGEAMFYGSGDQAFSNL
                     AKGLDVAGHEMSHGVIQSTANLDYEGESGALNESFADIFGRLIDRDDWLIGESVVKLS
                     AFPSGALRSLSDPHNGGLQFGDPGYQPRVYGERFTESADNGGVHINSGIPNWAFYQFT
                     IRINNDLDKAEKIYYRALTLYLTRSSRFVDCRRAVIQAATDLYGASGAEVNAAKAAYD
                     LVGILDGAPTPTEQNAETNPGEDFIVYADVNSTALYLADPAGNLVANPLSSKDPRPES
                     RTSVTDDGSAFVFVGQDKKIHYVIVDWNISQITDEGILQDQPLWRNVAISRDGNRIAA
                     LQDQEENRIHIYDFGLGSGGEWRTFDLYNPTYTSGITTGEVLYADAIEFDFSGEWLMY
                     DAYNSIPNTDWADINYWDISFLRVYDQTTQDWGDGRIVKLFSQLPEQTSVGNPTFSKN
                     SPYIIAFDMRVEDTDPFTYLLLGANLETGRLDTIFINSELAWPSYSTLDDKVIFNAST
                     QSGTEVIGQISVNNSKIEGTGTATALISNARQGNWFANGARDLSTPFKDQSLQAYAVR
                     LSPNPVSRDKIRVGWTQPKPSEGNRFAIYDVMGRLKWSSTGHFAGGDQTLDIPIHSLS
                     AGIYSLTLTIEGHTGSLKFIRL"
BASE COUNT        22788 a        21979 c        23020 g        21493 t
ORIGIN      
        1 tttggcacct ttatccaaaa aactaaagct taaaaggttg tagtgcaaca agcacggctg
       61 atagctgata gctgataacc gaaaaccctt atttttggaa ccgactctgg ccgttaatgg
      121 actactctgt tgatggcgtg cgcaatcatc cgaaaaagtc cattcatgtc agaaaaccgc
      181 cttatccagc tacaggtcag atcattaaaa ttattccgga aaatccaccg ctggacgggc
      241 atttcacttt ttgtattttt taccatcgtg gcgcttaccg ccctgatcct ggggtggaaa
      301 aaaaataccg gcgaactgat tatgccggcg acgaaaaaag gaagtacagc agagctcaaa
      361 gcctggctgc cgctggacag cctgcataaa atagcctgcc aggcgcttca tgattcagtg
      421 tccggggccc tttcgactga actggaccga atcgatgtca gggtcgataa aggaatcgca
      481 aaatttcttt tcatagagcg gtactgggag attcaattgg atggggctac gggaaaaatc
      541 ctaagtatcg gaaaaaggta ttccgacttt tttgaaaatg tccacgatgg cttgatcctg
      601 gaccgttacc tgggtacctc gggggaaccc atcaaactgg tttataccac cttaatgggg
      661 ctcgcccttt tggtttttac catcaccggg ttctggttgt ggtatggtcc gaagaggatc
      721 cgtcaccata aacacgcaca aaaaacaagc ctccctcatc actgactcag ggtttgattt
      781 taaagcggtc cccttccagg caaacatcca gccgggctga tctgaatcca atttttctcc
      841 ccggtccggt cctgatctta acaggccggc gggccactac tacctggcct tccccggtca
      901 cctccgcccg gcggccatgc accaggatgg cggtggattc atcgatccca atcaccactt
      961 tagcgggata ctgatgcaat aaggtaaata aacggttgta ccggcttcga acgataaaat
     1021 gctggtcgat gatcacttgt tcaagcaggc caaggccggt gcttgtttca atattgcctt
     1081 ccgccatccg gtccaccgcg ccttcataga gggtatcgcc gcgaatctga ttgcctgtga
     1141 tcattacccg gctcatgact gcggctcctg cactggtccc tgcgatcatg gccccagacc
     1201 ggtaggcggc atgaatggct tcagccaccc ctgtccctgc tatgctgtcc ataaaacggc
     1261 tttgaacgcc ccccgtgata tataatagac cggcacaccg gatagagtcc agaactgcag
     1321 gttgccgggc actgaccctg gtcaactgca tttttaagac cggattggcg caaagcacct
     1381 gcaggtcttt tttgatataa tgataggagg tgtccggatt tgcactggcc atggtcaaca
     1441 caaggatatt ctttcccggg cccaggtccg ctgttttgag cattttttcc atcatggcca
     1501 tgggccggtc ccctcctcct ataataaata agctgccttt tggaccagat tgagatgaac
     1561 cagtcactgt aagggccgcc aggatcaatg cattcaatac tccgggatat aaacgcatgc
     1621 gggaaaggtt attttacaat ggtaatttca agttcctgga tcaggggagg gtcttccgga
     1681 ctcagggtga agaatacgga gagttttcct ttttcccctt cgaccagata gcttcccctc
     1741 aattggtttt ctgcgatcaa cggaccgttg cgaaggatgg ggcctatggc tttgaaagcc
     1801 cgctggtgga tcgtttgcct ggtttccagg aaaacatctt caaaaaagtt ttcagcaaaa
     1861 atatttccgg cgcctgagct cagggccgac aggttttccg ctttccaatc cggcagtacc
     1921 tggatcaatt gtttttgcct tgccagcaat actggtgatg caggcacccc cctgggccgg
     1981 atcccggtcc gccggatcag gctgtccagt acgcgcagat tgatcgtacc caggtcggca
     2041 taggtgaggt tggcaaaagc gaccaagccg ataccatatt caggcaaaaa acgccactgg
     2101 ctaccataac ccgggaggcc gccgctgtgt cccacataaa ccctgccctc acaatccctc
     2161 atccagctca gtccgtagcc ataggccgta acgacggcac agggcctttg gccgggatag
     2221 cggaaagcag gatggaagcc tgaaaaattg acgggtagat gcatttcgcg aagactgctt
     2281 cttttaagca cgggattttc aagcccatcc cgcggaggcc aggcggaaag atgaagacta
     2341 aggtatttct ggaaatcccc gatcgaagtg atcaggccgc ccatcgcacc gtagacccca
     2401 tgatgcagga ggggttcttc aatccattcc tgcccgatcc tacggtaacc caaagccagt
     2461 ttcccctgcg gcacttttgt gtattcccat tccgtatgat ccatgcccag gggcttgaaa
     2521 atcttttccc gggtatattg ttcaagcggc tgaccggata ttttttccac gatctgaccg
     2581 agcaaggcaa agcccaaatt gctgtattca taggccaccc cgggtacatt ggaaaaccag
     2641 gtgcctttac ggatcaaggc gaccaggtcc tcgtgggtat cctccaactg gcggtcgccc
     2701 cagggattat cttccggaaa tccggcagca tggctcatca gatgccgtat ggtgatgggc
     2761 ggcgcatccg tggtaagcaa tgaattgttt ttcatcaatg ggatataaag gtctaccgga
     2821 tcatccagcc gtaatcgccc ggcatccctc aactggagaa tagccagggc cgtgatgctc
     2881 ttgctcatgg aagcgatgcg gtacagggtg gaaagatccg ccctgatcct ctggtttgta
     2941 ttggcctggc caaggcaacc ggacgcgatc agttgtccat cggccaccag cccataagcc
     3001 agggaaggga actgcttttc tttggcataa tcccggtaaa gactgtcgat gaaaggcatc
     3061 gcggctttca ttcgctgcag ccggtcatct tcgggataaa cgggttctgc atagctttgt
     3121 tttttttggg ccagtcccgg gaaggaaatg caggtggaga ccagcagaac cgcccagagc
     3181 ctggtgaggc attttgccat gttcttggat tttagaattg ggaagataac gaactcaggg
     3241 agcacttaaa caccaataaa attttgaggg ggaggctcgt ttatttatct tcgtcatttc
     3301 atttattccg gctaccaggt ggaaaacaaa gtaatggaat atacggttat tgaggatcat
     3361 gggtttaaat acattgagac gaatcctggc aaacccgtcc tgttgctgct tcatggtttg
     3421 tttggggcgc tgagtaattt taaagggatc ctgggttatt ttgaaaataa actcaatgta
     3481 gtggtcccca tccttccgat tttcgaactt cccattaccg aagtttccct ggagggattg
     3541 gtcaaacaca tcaaggcctt cgtgacctat aaggaaatct attccctgga tttgctggga
     3601 aattctttgg gcgggcacct ggccatttta tataccctga aatacccggg agaagtgcgc
     3661 tccctgaccc ttaccggaag ttccggcttg tttgaatcgg ccatgggaac gactttcccc
     3721 aagcggggcg actacgattt cattaaaaac aagaccgaac tgacttttta cgatccctcg
     3781 gtcgccacta aggaattggt ggatgaagtg ttcaacatcg tcaacgaccg catgaaaggc
     3841 cttcgcatcg tacttacggc taaaagcgcc atgcgcaata atgttgaaaa cctggtttct
     3901 tccattaccg cgcccaccct cctggtctgg ggaaaggatg attccattac tcccgctttt
     3961 gtcggggaac ggtttcatga actgatgccc aattcggagc tcgtccttct cgatcaatgc
     4021 gggcatgccc caatgatgga acacccccaa aaattcaacg aaatcctgga tggctttctg
     4081 accagggtcc acgaggtggt ctacggttga cctgttttta cctttctcct ctcttaagaa
     4141 aatatcacga ttctatcctt tgatagaacg aaggcgtcaa acctgcattt tcattcaacc
     4201 gttcggcagg tattcacgaa atttatagga aactgaaaac agaccttatg aagattacca
     4261 ggagatcctc cattcaaaaa atcatgtccc tgcccctggc tgttccgggt ttgtccatga
     4321 ggccccggga cccctttggg atcccgccgg tcccgggcca aagactgaga tccagcctga
     4381 atgcctactc cttcaacggg cctttgatgg aagggaagat ggacatagtc gggatgatgg
     4441 aatactgtgc cttgctgggc tttgaaggaa tagatatcac cggatattat tttaaaggat
     4501 atccgaatgt accggacacg gattatttgt tcggcattaa acggaaggcc catgccctgg
     4561 ctatagagat cagcggtacc ggggtccgga acgactttac aatcccggaa aaatcgaaac
     4621 gtgaggatga ggtaaggcgg gtattccaat gggtagaagt ggccgcccgc ctgggcgctc
     4681 cggtccttcg gatttttgca gggcaggaaa aacgggaagg ttacaaccgg gaacaaatca
     4741 ccgaatggat actggaagac ctccgccgat gtgtcgatta cggtaaacaa aacggggtga
     4801 tcattggact gcaaaaccac aatgacttca tccagaattc agaccaggtg atttcaatta
     4861 tgaaagcgat cccctctccc tggtttggcc tgatcctgga tacgggcagt taccggcagg
     4921 aagatcccta cctggctatc gaaaaaacca ttgactatgc cgtgaactgg caaattaagg
     4981 aaaagatatt cgtcgggggc aaggaggtgg aaaccgatat tccccggctg ttttccatta
     5041 ttaagaaatc cgcctaccag ggttatctgc ccattgaaac tttagggccc ggtgacccca
     5101 agccgaaaat cgaagctctc ctggaaaaag tgacgcgtga aatgcaatga tatggatata
     5161 gttagccagt tgagggcaac aaaaaaacag gccctgctct attttgatct gccccctgaa
     5221 gtctatgacc tgacctactg ccccggtaaa tggaatataa gacagttgct caaccacatc
     5281 gctgatgcgg aaaccgtttt gtacgaccgg atccggagga ttattgctga acagcccaat
     5341 cccctcgttt ggtcctttga ccaggatggt tgggcccggc acctgaatta tgatcatttt
     5401 ccgctccata tcaacaagca tatatttgct tccatacgcg atggcatcat ttatctgacc
     5461 gggatctatt atgaagacca tggtacactg cctttcatac atagccagac cggtgcacga
     5521 accctcaagg atgaaatcga caaggtggcc tggcattgcc gccatcacct ggatcaaatt
     5581 gaactcgcac tgcaggcgaa gccttttatt tcacaagtag attgatcttt cccctgaact
     5641 cagacaaata aaaagacagg atgcaggccg gctgggcccg ggctttttat cttgcgcccc
     5701 gaagatgagt aactcaaata agggatcaat tttccagctt gcagaattgg attttatgga
     5761 aaaatagttc ttaatcgtcc accacccacc atccaccaca cctattccac cgaaatctca
     5821 tccgcaaata taaatgcctt gcttcctgat ccgggatgcc agggagggca ggcgccgata
     5881 tttcgcgcca ccacccttac ataacggatg gtgttgttga ggtcggcagc atatacttta
     5941 taaaggggat tgcggtcgtt cctgatttct tccgtttgtt tcaggatttc attgtaagtc
     6001 cttccattcg aggaaacgct gatctccacc tggttcggaa gaaagatcca ggcgttttga
     6061 ttctgcagaa aaccggtctc gatacggcgg acgcgggtcg cctcgcccag gtccagcacg
     6121 acgtccaggt cattgccttc ccagccaatc cattctttat ccccgatggc gttgaggttt
     6181 gctttctgtc cgttgaccag gctcaggggg tcctggccgg ggtatttttc actgggaggg
     6241 ttattgtatg ccacggaccg gtaagccgcc ttatgcatca ggatctccct ggaggaaaca
     6301 ttttcagaca gaaggtcacc ggatacggat ttggccatta cccgggtcct ttgttttacc
     6361 aggaaaggcc cggtatagac ctgcgattgg agcccgggct ccgtgccgtt gaggctgtac
     6421 acgatcttat ccgcattttc cccctccagt gaaaccagaa aaccctggct ggtggaatcg
     6481 taaacggaat ttatcaggat attgctgtac accaccggcc agtcatcggt tctgaagggg
     6541 gaggcgggaa ggctatcgtg attatacagg ttgccctcgg cagtgttggt aaaagcataa
     6601 cgggctgcga caggttcagg gacctgttcg ctggacaaaa tgagctggtt cccatcgatc
     6661 tccaccttgg cttcgacaaa acgccggtct tctccggcaa ttctgtacaa gtccggcttc
     6721 tcccctttta tatacagccc gctttccgcg tgatcgaaag acacaatgac cttctggcct
     6781 tccaccttca tgcttttata cagagggccg ctgcattcaa gggtctggcc gtaattattg
     6841 gccagggccc ataaagccaa tcttttaccc acctcccttt tattcctggg atggatatcc
     6901 tgtacattgc caatatcggt cgtcacggcc atgccggtac ccggagacag cgacagggtt
     6961 ttaagctgtg cttcctggac cagggatccc tgaagcggac ggccgtattg aaagggggcg
     7021 atctgtacat aatagaaagg aaaattataa ttccaggctt gtcgccagcc gttgatcata
     7081 gccgggaaaa gcctccggta aaccagggcg ttgcccgtgt tggattctcc ctgataccat
     7141 aaagccccgg caatccggta ggggaccagc ggataaatca tggcgttgaa agtgctggcc
     7201 gggaggtaag gccaggggta gttttctgga aatatttctt tccaggaggc gaattcaggg
     7261 tctgtctcca taatattttt tggggtccag gcttccgctg ccgatccgcc ccaactggca
     7321 tggatgatcc ccaccgggat attgagcctt tctttcagct caagggcaaa aaaataagcc
     7381 acggcgctga aaccgggcat ggtctccggg ctgcagacct cccactcggc atcgatatcc
     7441 tcctgggggt agtccgacac ccttctgggt atgcggattt gacggatcag gggttggttg
     7501 ctgttctgga ttgcctggtc ttgattttcg atccccgaac tggcggtcca ttccatattg
     7561 gattgtccgg aacagagcca gacttcaccg atcatgacgt gatgcagcac gagggtattc
     7621 ctttccgaaa tagagatggt gtagggccct ccggcttcgg gtgttttgac caggatcctc
     7681 cattgcgctt tgtgatctgc aagcacctta taaatcacat tgtcccagct gcaggaaatc
     7741 gtgatggtgg cgttgggatc cgaccatccc caaaaaggga cctgttgatt ctgctgcagg
     7801 accatatgat cggaaaacaa ggcgggcacc cttacccggg catccaggtt aagactgaag
     7861 aaaaacagga taccaaacag aaaacaggct ctcggcatga cggtgattaa aaccatcaaa
     7921 ggtaaaggct ggagatgaat tacaaagggg atcccggagg ctggaatttt gtttaaaagg
     7981 agtgccggga atgggatttg aacctgcctt cgcgacgctg cggctgggat gctctaccgc
     8041 agagggtctg gaacaatatg agttttgttt taaaaggaag agccgagaat gggatttgaa
     8101 cccacggcct gctgattacg aatcagctgc tctaccgctg agctatctcg gcttaaaaga
     8161 aacaaaccgg gcgcaaagct aatgcacagg ctccatatag ggtcaggatt ggggtaaaat
     8221 gtatggcagc ccggttgaaa acctgaagaa ttacccttcg gggtgagcgc cggatgaaaa
     8281 aagttggtca aatttaagcc ttcttttaaa ccaatccttc cgaactccat ccaatccatt
     8341 gtaaactaaa tatgaaaaac atgaaaactc ttttgaactt ctggctcctg cttttactgg
     8401 tatcctcgca gttccaggtc cgggctcagt cctcggacga tagggattcc accggccttc
     8461 ccggggacca attcagcctt cagggcgctt tggaaatgtt taaaaaagcc tcctctccgg
     8521 aggagtttga aaaactgatc aataccgaaa ataaccatgt caacaatctg gacctcaatg
     8581 gcgacggcga catcgattat gtaaaggtga ttgaccatat ggaccaggac gtgcatgccc
     8641 tggttttgca ggtcgccgtt tcggccaatg aaaaccagga tatcgcggtc atcgagattg
     8701 aaaaaaacgg gacggaatcc gccgtattgc aaatcatcgg tgatgaagaa atctatgggg
     8761 aagccgtaat catggaaccc cagggcgagg aaggaaaaga aaaatcccgg aaagggccaa
     8821 gcctgcttga ggacgatgcg atcgtaataa acgtttggtt ttggccttcg gtgcgttata
     8881 tctacgctcc tgtgtaccgg ccttgggcat caccctggta ctggggatat tatccgaact
     8941 ggtggagacc ctggaggcct ttgggatggc atgtctggca tccgttccgg gtccattatc
     9001 acgtgggtta tgtctttgca cccacccacc gggtcgttcg cgcacaccgg gtttataccc
     9061 cggtaagagt gacctcggtc accgtaaaaa ccagaaatca ccaggccgtc agcaactaca
     9121 gggtcaaccg gtcaaaaacc acggtgaccg cgacccgggg caataatcag gtcaaagctt
     9181 cccggaccca aactacggtg tccggtaaaa aagggaatac ccgggggacg gctaccagga
     9241 cctcaacgac cgtctccggt aaaaagggca ataccaaggt aaacgccact aaaaccagga
     9301 cttccgtcag gaagaggaat tgatttggga aaaagcaaaa agcagggggt gtctcgaacg
     9361 acaaaaagaa agggggtatc tttcaagaca ccccctttct ttaagaagca caattatctt
     9421 acctcaaaat ctttcgtaaa cactcgtccg gaaaggctga cgtcaaaata ataatttccg
     9481 ggttcgagtt tttccaggtt aaaccggcgg gtaacgctga cctggttgtt gaaccgctcg
     9541 gaatgagcca catcgccctg tgcattccgg atgctcagga cggctttgtt gccctgggtc
     9601 atcaggttgc aatccagttt gtcatcatgg atcttgacca ccggtttaaa gatgatttca
     9661 gaatccttgc tgaaaaggaa gccttttgaa atcgacagac gctggatgct tattttctga
     9721 tcgtcgttga tttccatgac atattctccg tcgggtacgt atttcagatt gtatttggtg
     9781 gattgtttga tgctttcgct gaccagcacg ttcccgttca cttcccggat ggtgacgtcg
     9841 atcacaggcg atttccattt atttttttca acaatgatca gcttggtgcc ggaaaggatt
     9901 ctcaccggta tttcagcgga cagcccggta ttcaccgcgg cgaaaaggat taaaaacaac
     9961 aggtttttca taataatagg atttttaagt ttatttaaat cattccaagt gaccggagcc
    10021 gacaacgaaa gggctgcaca cggcataacc cagccgggtg catacacgtc gttaccggac
    10081 gatgcacgga tttagagaaa ttctttccaa taaaaattta aggagtcttt gctgtttgga
    10141 tcagaacaac cggagaatat gccctatccc cgttttataa acctgttctg ttccgattta
    10201 gaggagtcaa acaacaataa tgatgacaag tttacataaa aggttgcatg cttgtcaagt
    10261 attaacggcc cggaaaagga gtttattcga caaaaatgga ttttaaaccc gatcatagac
    10321 caatccaccg cagaaatacg tgatccttcc gggcccttgt cctgcaaggg ctgataaact
    10381 cctcctccgg ggcggcagag gtttaaaaaa atacaggcat taaaatttat ttaaaatttt
    10441 ttagcccctg cctgtaacct ttgtgttaat ttcctgtatt catttcagaa ctaacaacga
    10501 gcaaggggtt tacatgtcgg atggggtaat catgcagcag gtgaaagcgg gtgacctgtc
    10561 acaggcttca gtccttttcc ggaaatacca ccgcaaaatt tattatttcc tcgggaaaat
    10621 gagcggggat taccacgcgg cagaagacct cacccaaaac gtatttgtca agatgatcca
    10681 gtacaaaaac agttataatc ctgaaatgaa cttcgaatcc tggatatacc ggatcgcccg
    10741 gaacagttac ctggatcatg tccgccagaa aaaaaacctg gtgtatacgg acctggagcc
    10801 cttccaacag ctgagtgaga aggatgaggc taatccggaa gaacaatacg accggcttca
    10861 ccaggcgctg gccggcctgc cttcggatga ccgcgaagtt ctggtcctca cccggtttca
    10921 gaaaatgaaa taccaggaag tcgctgccat cctcggtacg acggaatcca atgtcaaagt
    10981 aaaagtgttc cgcgccctgg aaaaactaag aacctctttt tttcaactcg aatcaaacca
    11041 gtgatgaacg aacaggacct gatcagggag aaactcatcg attatattga tggacgcttg
    11101 cccgaagcgg aaaaggggga aatccaacgt acgctggatg aaaatcccgc cgtgcgggaa
    11161 gaatacgaac aactgaggat cctgctgcac gggctggaag aggaactccc tgggatccct
    11221 tcacagaaac tcattggccg cttcgaagac tggctggacg gctaccggtc ccaggccagg
    11281 cctgaatcca aatccctgtt tttttccacc tggttcaagc ttgcggcctc tctcttgctg
    11341 ctggtccttg cgggtggagg cggttatctg atcaaatccg ccctggatca gagggctgag
    11401 gtggcgaagc tgcaaaagga actggccgaa accaaaagcc tggtcctgaa tcagctccag
    11461 gacaaacaat cagccagtca gcgcatcctg ggggtttatg cggccaacga actgcacacg
    11521 gccgacgatg aaatcataaa agtcctggcc aaagccatgg aggaggatcc gagttccaat
    11581 gtccggatgg cggccctgga agccctcagc aggttttacc aggacgaacg ggtgagaacc
    11641 ctgttgatcg gctccctgaa ccgccaaacc gacccggtag tccagatcgc cctgatccag
    11701 tggctggtcc agatgaaaga aaagtccatc ctgcaggacc ttgagaaaat tacccgcggc
    11761 aattcaccgg tgaaagcggt gaaggacgag gcttataaag gcatattcaa gttaacttaa
    11821 tatacattcc atcactttct aaactcaaat ttttaaaaac aaccaaacat gaatactgga
    11881 aaaatactgt ttaccctctg cctcctgggc atggccgcag ccttgcaagc tcaggaatac
    11941 aaagttgcca agacttcagg ccgcctggaa ctgcacatcg gcaacgtcac ggtagagggc
    12001 catgccggaa atgaaatcat attctcgtcc aaagacgggg aaaatgaaaa ggatgaacgc
    12061 gcagccggac tcgtagccat caatgccatg ggcatggagg acaacacccg cctgggcatt
    12121 aatgtttcag ccaagggcga tgccttggtc gtcagccctc tttctaaaac aaagtccccg
    12181 gagatcaggg tcctggtacc caaaggagtg attgtctcct acgattacga atcccaatac
    12241 ggagggaaag cgaggtttaa aaatctggaa aacgagctgg agatctctgc cacctacaac
    12301 ggcatcgaac tggaaggcat tacgggcccc gtgacggtca aaaccatcta cagtcccatt
    12361 gatgcggaat ttgccaacac cgtgaaagga cccgtgtccc tgatatccat ctacagcacc
    12421 gtcgatgtca ccctcccgca aagcctgaaa gcggatctga gcatgaaaac ttcctacggc
    12481 gagatgctgg tcgcgccaga gtttaagatc gaaatcctca gaccttcgga aaaagaaggg
    12541 gacatggtct ccatgggcgg caataaagtg cagggtaaaa tcaacggcgg gggcatcaag
    12601 gtggatctcc ggtccgacta cagcaagatc tacctgagaa aaaaatagga tgaccggaca
    12661 ctgattttct caccagaaac aataaaaatc aatgaaaaaa atactgttcc tgctcgcagc
    12721 gggctggaca agcctctgtc ctgcacaaag ccccgtgaat aaatccattc cggtaaattc
    12781 cgggcaaagc ctgcagttgt attttgacta ccccaacctc atccgggtca gcagttggga
    12841 caaaaatgaa atccagatta ccggatcggt cagcatcaac aacggcgaaa atgatgatgc
    12901 ctttgtcctg acttcggcgg tatcaggcgg ccaggtggtc gtcaaaggtg aaatccccga
    12961 tatcaaatcc ctgccccagc agatcaccct ccggcgggga aatgaaaaaa tcgtattcaa
    13021 aaacaaatcg gaatacgaga aatacaagca ggagcacggc gcttcgtttg atttcatgaa
    13081 caggggctcc gatatcgaaa tcagcctgga tatcaaggtt ccggccagct tgccggtaag
    13141 cgtggaatcg gtatacggga tcgtggaatt gaaaaacctg aaacaatcca tgcccttgtc
    13201 cgcgatctcc acctatggag gtgtggatgc agccctgcat gcggcacagg tgggggaatt
    13261 gtacgcgtcg accgatttcg gccagatcta ttccaacctg gatgtgtcct tcagcgggga
    13321 gggattaaaa caaaaggatt ttcataccga gatcatggcc cgcccgggta aaggcgcccg
    13381 ctaccgtttt gaatcaaaat acgggaacgt ttacctcaga aagaactgag taccgtattc
    13441 aagccgcctg aacgaacaag gaaataccgg acacggaaaa gtaaccttta ttctttgccg
    13501 gagttgtcag gatatgttcc gtcgcttaac acaattttat gatgattcat tcacctttcc
    13561 cccttttagg gtgaatgacg aaaaggatgc gccggatacc tttgacagaa tgaaaacaga
    13621 attcctgaaa gccggatgga aaaaagttca taggctggat ggacgcggga ttcgccaccc
    13681 ggaacaggaa gcatgaacct ttcgaaattt agagtgagtc ttttcaataa aaacccgcag
    13741 ccttcctaac cccggaaagg ttgccgggtt tttttcttta aggagcattc agggcattga
    13801 tgattgaaac aggggaaaga ttttttcaaa tctttattca agatttaagc agggatattt
    13861 gaatttgaga tcctttgccc gggtctccat tttttcgata aaggacttat gttccgggct
    13921 tccgggatgc aagggacgat gggaccttgc ttcaaccagc cggctcacct catccgcgat
    13981 ttgccgcgtt tcttttgaaa aataggtcga ctgaaagcag gtccagatgt attgaacggc
    14041 ctgttcgttg ggatggacca tatcttcttt ataaaaccgg taatcccgca gatcgtcgag
    14101 gaggatctca taagccggga aataaaagat ggactcattg cgtcggcaga gttgatccgc
    14161 ggccagcacc agggtggcct tgctgatgct gttgtccccg aaaccttctt tgaggtaacg
    14221 aaccgggctg accgtgagca ggatttttat tccgggcctt cgttttagca gttcaggaag
    14281 ccatgattcc acggattgca ccacctcttc cacattcata aaccctcttt caaattcact
    14341 ggtgggaatc ttgtgacagt tggccacggc ctggccgctg gctttatgtt tgtaccagat
    14401 cgccgtaccc agggtcagta tcaacacatc catcctttca aaccggtccc gtgcttcgtg
    14461 gagggaatca ttcatcctgg aaaggcagtc ctcctgccgg ggggaagaaa aagaaccgtg
    14521 atgatgtaat ccgtggacca gtccgcgatc atggatgagg tcctgctcat tccatgccgg
    14581 cccgtccagg atgcgcctga tctgcaagcc gatacttatc ggattgtaca cgatgccgca
    14641 gggattgcag tcgctgtgaa agccgcctgc cttgaaccgc agatgcagat gctcggcgaa
    14701 acacgaaccg atgcaaaaga gccggtcctg gtgttggatt ttccagggag aaggcgacag
    14761 tttgatttcg gttctaaaaa caggcccggg catggccgca aggtagttgc atttgaggag
    14821 atcctttact tccttatttt gcgccttatt tgaagattga acatgagctt ttttgaccgg
    14881 attttcagca aaaaaagcca gtctgcggat accttgtgtt acgaagccag tttcggccgg
    14941 tataccgatg cttataaatc cgatgcccag tttgaagcct gggaccgggc catcgaacaa
    15001 tttgaacaac aggaatacag gaagtccctt cttgatttta tcgaatacat caaaaacccg
    15061 gagggttcca atatcatcct gcacagcgaa caaccggtgt cttttgacat gcttcagggt
    15121 tccaaaacca tcgaatgccg gttgaagagc ggggtcgtat ttgtggaatg ccgcatcgcc
    15181 gccgtttcgg aaatgaacat tgggtttatg cggcggctgc tcgatctcaa ttatgagttg
    15241 aaatacgtga ggtacggatt gacggaggac cgggtcgtgg tgctgcgctt tgatacccca
    15301 ttgaccgatg cctcccctta taaattgtat tttgcattaa aagaactgtc catacacgcc
    15361 gacaaacagg atgaccttct cctgcaggag ttttccttcc ttcacccggt ccggtccggc
    15421 aagatcgaac cggctcttcc tgccgaagcc gaccacaaac tggccttttt caggtctgcc
    15481 atcgacaaag cctttcatct tgtggatcat ggcaaactga aaaccgaaca gtatccggca
    15541 gccaccggat acatcctgct ggctgccatg tataagctgg actatctcat ccggccggaa
    15601 ggcggccttg tggaccggat agagcagatc cataaaagct attttgccca ggaccagaaa
    15661 aacattcaat taaagctcaa gcacctgagg caggaaatgg tcaaactccg gcagatgagc
    15721 gatgacgacc tcaaagcgga gatctaccac accatttcca cctttggcat caccacgccg
    15781 gcaaccctgc cggtgctgca cgaactcatc gaccgggaga cgcagtccat tcaatggtat
    15841 atcgaccagg aatacaacga atatgcactg ggcatggtgg aatacattgc cggtttcagc
    15901 ttgttcaatt ttgccctgcc cctgccggcc aaagacctgc tgcacctgat tttcagggtg
    15961 accgaagccg aatttttcaa gaacatcaaa ggaaatcaaa ccctgtacga ccggggcaaa
    16021 atatcgaagg cggcggtccg ggccgaattg aaccggctgg aaagcatcca ccagaaaaaa
    16081 tacctggccc ttgctttcga tcataaaaaa ctcaggtacg attcgccctt aacttttgct
    16141 tacggcatcc tgcagatggt ccggggcctg caacttcaga aagcacgatg aaagacatca
    16201 ttgagatcta taaggattat gtggtacaga tcgcgacccc gtacaatacc ggcacgggat
    16261 ttctactgcc ccgtgaaggc atcatcgtca ccaacgagca tgtggtccgg gacaaccacg
    16321 aagtggtgct ggaagggcgg ctgatcccaa aacaaatggc ccgggtcctt tacctcgacc
    16381 aaaagtttga cctttccttt atccaggcgc cggcattggc ccgggaggtg gaaggcattg
    16441 aaatagaaaa aacccggcgg ctccacgaag gagacctgat catcgccgtc ggccatccct
    16501 tcgggctaaa atataccgcc acccagggca ttgtgtccaa caccatgcat atgcagaacg
    16561 atatcttgta tatccagcat gatgcggccc tcaatccggg caacagcgga gggcctttga
    16621 tcaatgccca tggccgcgtg gtgggggtaa acacctttat tgttcaaaac ggcaacagca
    16681 tcggattttc ccttcccgcc agttacctgg aagaaaccct tgccgccttc agccagggcg
    16741 ggggggacga ggccacgcgc tgcgaatcct gttccaacat cgtctttggg aaatccagta
    16801 ccggccatta ttgtctccat tgcggatcca aaatccggat gccttccgat gccgagcctt
    16861 atgaagccat cggcatgcag cggacgatcg aggataccct gatccggctg ggatatgcag
    16921 tgaacatttg ccgccggggg ccgaacaatt gggaactggt tcatggcagc gccaccatcg
    16981 aaattgccta tcatgaccaa agcggtttga tcctctgtga tgcggtactt tgttacctgc
    17041 ccaaaaccca gatccaggaa ctttacgagt atctcctaag gcagaatcat caaatgcagg
    17101 gaggcagcct tagcctgcgg ggcaatgaaa tcatcctttc cacgatcatg gtggaccgcc
    17161 agcttgatgt acaacagggt taccaccaga tcaaaaacct gatggaaagg gcggattatt
    17221 acgacgacca gctcgtcaat ctgttcgggg cgatctggaa gcaggaagac tgaccgtcaa
    17281 ttctggacaa aaaccttttt aacgactttt tcgctttcga tcaggtcaaa gaagggattc
    17341 aaatatttct ttatccctga cctggatttt tcatcaagca gggtaaaccc gtccaccact
    17401 ttgaaaagct cttccttctt gctcataaaa tatttggccg tggaaagcgc ttcctcttcc
    17461 gtaacgccgt gtcccatata atagcgctca taaatggatt tgatcggaag cgattcatgg
    17521 ggcactgcat agggcgtgcc caccagggcc gcgtaatcga agtcgtaggg gatggggatg
    17581 acctttgcca cggcgggcac tttcaccatt tctacgttgt gtttgttggg gatggaccag
    17641 tcggtattgc cgatcatgta ctggaaaaaa accattttca ggtaattgtc cttgtccagg
    17701 gccgggacca tgaccttgcc ggattcgatc actttccctc ccatgcggtc ggcgatctct
    17761 ccctcatcct ccacaaaaag gccatccagg gtgtatttta ccttgccttc gtcgtagcat
    17821 tcgaatttga ccagtttatg gtggacaaaa taatccggat tgaccacccg gtacaggtcg
    17881 tagctcaatt tttcccggaa gaggaattcg gaattggctt cccccgtacg gcattgaaat
    17941 accactttta atttgtcgat gctggtatca agggcctgtt tctccagctg cccgttcttg
    18001 aaatccactt tcacaggcgg gaaaaaacaa acctgcttcc gcatcatccc ccttgcccgg
    18061 acgcggatgt caaaatccac gggctgtccg ttgcagggaa gggtgatctt tccgggcaag
    18121 tactcttcct gcattttctg gttgatcagc ttattccaat tggtttcgat tttaataacc
    18181 ggaacccctt ctgcttcgag aacacagtca aaaatggatt tggtatggcc attctgggct
    18241 gaaaggctcc cgaaagtaaa aattagaagc aggaggatcc aaagagggga tttgagggtc
    18301 tttttcattt caaggatatt tgatttagtg aagagggcct acccggccca aagggcatat
    18361 aaaattaaat aaaaatttat gtatttattt tttaattccc ggatgtgaat accgccgcct
    18421 ggtatagggc cttcgttttt ctatacgggt aatttgaatt tgccaaccgc gggatgaaag
    18481 cgggagatgg gcctcgcagg gtcaggttcc ttgggaatca atgtttttga agcactggcc
    18541 ggggatagca tcctcctcgt acattaaact ttgaccattt gctccggtat ttggaggcag
    18601 gcgcccggtt agagttccgc taccggcctt acctttgaaa cggggagggc ttccccggtt
    18661 catttattgg tatgaaaaat tgtttttatt cctgccttct tatccttctt ggggtggacc
    18721 ttgcagcgca ggtccagcga tatctgtatg ttgccgatga ccagggctgg atttatattt
    18781 acgacatcaa caaaaaccac cacctggtac gcaaattcga agtgccgggc accggggaat
    18841 acaagggcat cagtgcggat gctgcccggg aacgtttgta cctgagttcc tataccggag
    18901 accagtttgt atgtgtggat ttgaaaaccg aaaaaatact gtattccatc cccatcctcg
    18961 gatacccgga cagccatgct caaaccccgg acgggaaatt tatatatctg cctaaaaggc
    19021 atgggaggag ctgggatgtg atcgatgcag accagggaaa gatcgtagcc aatatcccgg
    19081 ttccctatgg caatccgcac aatacctggg ccggcaagga cggtacccgg atgtacctcg
    19141 ccgcccttgg taatgaaaac ctatacattg ccgataccaa aacacattcc atcacgggcc
    19201 aggtcggtcc tttccagccc aatcccgaag atccctgggg atggactcag aaaaggcaca
    19261 atggccccaa agggataagg ccatttgcgg taagttcaga tgataggttt tgttatgtca
    19321 acctggatgg catcctgggc tatgaaattg gagacatcag tacgggaaaa cggataggca
    19381 gggtcggggt ggaaggtttt gaaaggaaaa gaggcagcca cctcaccacc agccacggca
    19441 tcaatcttcc cccggatgaa aaggaaatct gggtgtccaa cgatgcagga ccttatctcc
    19501 atgtattcga tgttaccgcc agcccgcata aaaaaatcgc ggatatcaaa ttgtccggta
    19561 aaaatggctg gataaccttc agcatcgacg ggaaatacgt ttatccttcc tcaggggatg
    19621 tgattgacgc cgcgtcgaaa accattattg cgaaaatcat tgaaagcgaa aagttattgg
    19681 agatccagtt tgagcggggg gtgccggtga gggctggcac aaggtaagtc agtggatggt
    19741 ggttggtggt cagtggttgg aggtcagcca atccatggtt ttttggtgga aatcggacgt
    19801 aacatcagca aaggccgtca ggatggaaaa gtgatttaaa ccgggccaga ggtgtttttc
    19861 aatagaaacg gatttgtttt tccatgccga gtccatttct tccaattggg agtggtattc
    19921 tgcagattcg tctccgccta cagccaacag gattcttgaa tcaggtatgg gatccagccg
    19981 cacaggactg aactcttcag ccgtcgaggt gtccatcccg attttgtcat tgacaaatga
    20041 caattgaatc gggaccagat cgtacaatcc gctgagggcg cagatgcctt tcaaccggac
    20101 ccccggcgga ccaaacttta ccgtagtcat catcgccgcc aaatgaccgc cggcgctgtg
    20161 cccggccagg tagagggatt ccgggtcccc gccgtattca ccaatgtggt cagcaatcca
    20221 aaccagggcc ttagcgcagg atgcgacgat ttcatgcatc gaagccgaag gcgcgagagg
    20281 ataagaaatc aggatcacat ggaaaccgtg ggggagaaat gcaggcgcaa caaaccggaa
    20341 attggacttg tccatggact gccagtatcc gccgtggatg aaaaccaggg ttttcccatt
    20401 gggcctgggg gcgggataga gatccaggat ctccggcttg ccggagccat aggggagatc
    20461 tttataaatc aggcacgaac gaagggcttc ctcacttttt tgttcccaca gattcaaatg
    20521 atcggcaaaa tcgggtacca ggccccggtt gttgtactgc cggtcgagtc cggcctggtc
    20581 atactcctta tagataaaca tgttccgagt ggaagggatt cagttggcca atgccttcct
    20641 cgtttggtta aggctccgga tcaccagccg gtagaaatcc ggatgggatt gccaggtttt
    20701 cccggtcact agatttccat cctgcaccgc ttcgtcccgg tccacatagc gacctccgca
    20761 ggattcgacc tcgaaacgca cgtgttcata acaggtcacg gtttttcctc ccaccagccc
    20821 ggcagtggtc aggatctgga tgccgtggca aatagcaaaa atgaacttgt ctttttgatc
    20881 aaaatgccgg accagttcga tgacccgggg attgttgcgc aggtattccg gggcccggcc
    20941 cccgatgcaa aggacggcga cgtattcttc cggccgaacg gactcaaagt cggtgtctgc
    21001 cgtgacgagg taaccctttc tttccacata ggtatcccag cctggctcga aatcgtgaat
    21061 caccaggttc agggccttga ccgcgggggc cgcgatgacc gcctgataac ccgcttcgct
    21121 aagcctttgt tttgcataaa gcacttcaaa actttcgccg gcatccccgg tgatgatgag
    21181 tattttacca gacataggtt ttgttcattt gttttatggt gaatagttcg aaagataaaa
    21241 tttatcttgc aaaaagggga aaaacttaat ccggatccgg gaggcttccc ggatccttct
    21301 aatcattaaa gcaaaccagc tatggatcct tctgaaagca atataaaaga ccggtttacg
    21361 attcagaaaa tttatttctt gacaggcatg gtcttattcc tgatcatcct ggagtcttgc
    21421 cggagcacac ggataaccag gcccttggcc gaccggtaca aagtcgcagc cgatgacagc
    21481 accctctatg ccgaccaggc gccatacatc atgccctata accgcgtgct ggatcctgcc
    21541 ggggtatcgg tgccttttgg tgatggtaat tatgaaaacc acagtctgga tgccatcacc
    21601 ctggccgatg gcaaaaccct cgtggtggag gaccggtacg gcctggcggt tttcgatatt
    21661 catagccgga aactgatcga tcggtggacg tttaaccggg atagcctctt cagcggcttg
    21721 atgagcagct attccgggat tacttcaata gtgcatcggg attccacttt tattttttgg
    21781 ggagccggag gaaggaatat tgtgattgga gctgagaggg ccaattcatt tgtcatgcag
    21841 gcatcctggg atgggaaaaa aataagaggg gttcattcgt atccgttcca ggccgaagcc
    21901 ccggccagga ttgctttacc caaccaggtg attgcaaaga ttgaagaagg cgaactttat
    21961 ttttataccg ttttaaatgg aaataataaa atcgttaagg tcagggtcag agatcggcaa
    22021 accatatggg aaacaaagac cgggatggct ccatttggac tggaaatcat cggggatttt
    22081 gcttacgtca ccaactgggc agggcccgtg ccgggtccgg atagtgtgga aagggcgggt
    22141 attccctggg gagaggccta tacggatccg gtgaccgggg ccgtttcccg gggaagcgtt
    22201 tctgtggtga atattcacac cgggcgccca acgaaggaaa tcgaggtagg ccttcatccc
    22261 aacgccatcc tcagcagccc ggataagcgg tttttatatg tcgccaacgg caacagtgat
    22321 tttatcagcg taatccaagt cagtacaaat tctgtaaccg actccattcc cgtcggtttg
    22381 ttcggaacgg agaagggtta tatcggaagc acgccgaatg ccctggcggt gaatcaggaa
    22441 gggaacagac tttatgtttc caatggattg gacaatgcga tctgtatcat cgacctggaa
    22501 gagacgccat cagggaaaaa ttacagggtc ctgggttaca ttcctacgga agcctatcct
    22561 tccggaatag tattgcacgg ggatcagatc atcgtcacta acctggaatc gaccggggca
    22621 agggcttccc gtaccgttca ggttggggat aaaaattcaa aaagcctgaa aacatttaat
    22681 tcccatcatc agctggcttc catttcattc ataccgcttc ccggtgccgc aagactcaag
    22741 cgatatacgg aacaagtaaa aaagctgagc atggcgcacc ggttggcgct cacccaaaga
    22801 ttacccaggc ccaatattgc cccgaaaccc gtgccggaac ggataggaga gccctccgta
    22861 ttccagcatg tattgtatat catcaaggaa aaccgcacct atgatcaggt ccttggagat
    22921 atgcccgaag gaaggggaat gccttctctt tgtattttcg gggacagcat taccccaaac
    22981 cagcaccaga tcgccaggga ttatgtactg ctcgacaatt atcatgtgtc cggtaaagga
    23041 tctgcggaag gacatcactg ggccagcgcg ggcatggtga ccgattatac cgaaaaaagt
    23101 gtccgggcct ggttcaggag ctatccgcat gtattgaatg atgccatggt ttacaacaaa
    23161 aaaggattta tctggaacaa tgccctggat catggcaaaa cggtaaggat ctatggtgaa
    23221 gcctgcactt acgaatatga caaaagcaag tacaactgga aaaccgtgta tgagctatgg
    23281 gaaaacggga ggtccagcga gttggccggc aaaagcacca cgaccatttc ccgggtaagg
    23341 ccgattttgt cccaaaactt tccgggcggg gacgatcaaa acatcaatga acagatgcgc
    23401 gcggatgcct ttatcaagga gctcaatgaa ttcagcgcat tgcccggaga ccagcttcct
    23461 aaccttatga tcatggccct gccgaatgat cacaccgcgg gtaccagtcc cgcttttccc
    23521 acgccccggt ctatggtggc tgacaatgat ttggccgtgg gccggatcgt ggaagccgtc
    23581 atgtccagcc ggtttgccga aaacaccgtg attttcattt cggaggacga ctcccagtcg
    23641 ggatgggatc atctttcttc ttatcgaact accggatttg tcgtcagcgc ctattcaagg
    23701 ttaaaaaaaa ccgtgcagac caattacaat caaacctcga tgcttcgcac gatggaacaa
    23761 attctcggac tgcctcccat gaatgtgatt gatgccacgg ctttgcccat gtttgattgt
    23821 tttagcgacc ggccggacta cgcctataaa tataagacga taaaaaacag gatcccgctc
    23881 aatgaaatga atccacaaag gcaaaatttg cagggagccg atttaaaatt cagtgaccaa
    23941 tccgtcaaat acatggttca tctaattgac cggggacatg atgatatttt aaaccggata
    24001 ttatggcgtg cggtcaaaag ggatcttcca taccctgacc ggtttacagg agaatcagat
    24061 aacgaagatt gaatacaaca tcgttaaacc tttctggata atttgattaa tttggataaa
    24121 agcgttgaac agccgatgaa aagaatggtt ttttatctgt ttgcctccct cctgcttaat
    24181 tcctgcaatt cggttcagga ggggtccccc ggttcactgg tcttggttca ggatgtggag
    24241 cctcaacccc ttcaggcgca ggtgatcaga ctggtggaag cccttgattt tatcggcagt
    24301 cctttgcatg aaaacgataa ggagcttttg ctttccttaa aaaataaaat accggatgat
    24361 tcaagcgcaa ggatgatcca gaccattctg gacccttatt gcctggccat ggtggagatc
    24421 aacccggaag ccagggtgaa ggtgatccgg ggacctgccg aggcacgcat cgtacaggcc
    24481 ggctggacca gttttatagt caaggtccac aatgcaggga atgtacacgc cagcctgcaa
    24541 gcggacagcc ccaatgcctt gctgcccttc agttcgcgct ggttatggag cccgacgcag
    24601 ggcaattttg cggacacggc cagacagccc cgaagcgcgg ctgaaacggt cagccgtttc
    24661 ctggagctgt ccttttacaa aaaaccaccc ctccttccca atctttccgg cctcagcctg
    24721 gaatacatga tcctgcagat atattgcaag gacgcgggca aacgggaggc tgagcttggt
    24781 tttaatatcg gacagggctc gcaggacctg ggtttcagaa acaccattcc cgttttgttt
    24841 gaggccaggc ctgccaaaaa agttatttta aaaatccggg acgacagcaa tcccaggggt
    24901 atcgcttcct ttctgatcac ggacggcatc gaacgcatat ccccggacac gatgcatcgg
    24961 gatcaactgc ctgatgaaag aacctgggcc cagatggagt ttggacaacc ggggagaggc
    25021 cgtgaaacgt atgtccggcc caaaagactc agcgggattt atcccctgcc ttcccgcagg
    25081 atggcatcga tggatgaatt tcccgatttt ttcttccaac cgcagatcta ccggttcgac
    25141 ggagaacacg tctatctccc ttcgggaaca ttcaacatcc gttattcccg ggggccggag
    25201 tatatcgaac aaaatcaaac cctggtggtg gatggatccg tggattcgct ggtggcaaat
    25261 ttcaccctga agcgctgggt ggatatggcc gccctgggct ggtacagcgc cgatcaccat
    25321 attcatgccg cgggatgttc acattacgat aagccggaag aaggggtaag ccccgaagtg
    25381 atgtggcggc aaatcctggg tgaagacctc catatggctt ccgtgctgac ctggggaccc
    25441 ggctggtatc atcagaaagg caactttacc gggcagtcca gcccgctttc gaccgaaagc
    25501 catgtgatgc gttatgacgt agaagtatcc ggatttcctt cccagcatgc cgggcatgtc
    25561 atcctgctga acctgaagga agacgattac cccgggacct ccaccacgga acaatggccc
    25621 acctggacct atccggtatt aaaatgggcg aaggagcagg gagcggttac gggatatgcg
    25681 cattcgggat ggggtttgga acccgtggag ccggtaaccg gtcttcccaa ctttgtcctt
    25741 cctaaaatgg acggcatcgg ggccaacgaa tacatcgtca cggtcaccca gggactgatc
    25801 gatttttaca gcgccggcga cacgcccccg ctttgggaat tgaatatgtg gtatcatacc
    25861 ctgaactgcg gattcaggcc ccggatcagc ggggaatcgg acttcccctg catctatgat
    25921 gaacgcatcg gcatgggccg cagttatttc aaaccctcgg ccgatttgaa ttttgatcag
    25981 tatacggagg ccatccggaa aggcagatct tatgtttcgg acggggggtc gcacatcatc
    26041 aacttcaaag taaacggtct tgaacccggg ctggaggaaa gcgaattgaa actttcaggc
    26101 ccggcaactc ttcagatcga agcggacctg gcggcttatc tgccggaaca gcaggatgaa
    26161 tccggaaggg ccatcgcagc caaaaggatg gacgaagcac cctattggca tattgaaaag
    26221 gcccggaaag gaaataccca taaagtcgaa gccgaactga tcttcaacgg gaaagccgtg
    26281 gataaaaaag aggtcacggc cgacggcaat tggaataaac tggcctttca atacccggtc
    26341 agccgttccg gctggctggc aatccgggtg ctgccaagtt cacataccaa ccccgtgttt
    26401 gtggtggttg gagaccggcc ggtgcaggcc agggaaagcg cagaatggtg ccggagggct
    26461 gtggatcaat gctggaagat gaaaaaggac aggatccagc cgaatgagcg gaagatggcc
    26521 ggtgaaatat accagaaggc cagggagcaa tacgaacgga ttgcatccgc agcggatgca
    26581 aaatgactgt ccccggacgg aaaagggtct ttctcatcac tgatctaagg gggttttggc
    26641 ctgggtgatc cgcccgatgg tatgccggtg tattttgcca tcctggatca ggtaagtggc
    26701 ggtcgaaaag ggcaccacca cggaattcgt ctccccatgc cagatggtat atccgagatc
    26761 gccatccaca atcagggtat ccaggatgat ctcggcctcg ccggcgggaa agttgttgat
    26821 tgcaccggtc agcaatctcc ggatttcatc caggccgctg gccacgatat ccagcatgat
    26881 taccttggaa ttttcggtat aattgctcat cagggcttcc aggtcgttgt tgacaaagga
    26941 gttgaaatgg tgttcgatga tgaactgggt tggatttgaa tgcataattc aaaaaggtta
    27001 atgagggttt gaatatttta agccggtgca aggtcaaagt tcgggaaaac cggaacagtt
    27061 tggttgatct gatccaccgg tttttcaaaa aagggattgg agtggggcat cctgcaatcg
    27121 gggattgcct accttttcgg atattgggta attcttgttt tattcataat gcagcttccg
    27181 ggtttgttgt cgaaaatagg attgtgcctt ttgatcttct gtaccggcgt gattcatgca
    27241 caatggagga gtcccaggct tacccatctg ggcccgctgc aaggcatgac ctcctggact
    27301 tttgaggtca cccaggattc aacagggtat atgtattttg gcacggacca gggcttggtg
    27361 cgttatgatg gaagccggtt tgagctgttt gcccacaatc cgaacgatac ccattccatc
    27421 gggcccgggg atgttcaatc ggtaatggct tcatcgggcg ggctcatctg gctgggtacg
    27481 cggctgtcgg ggctgcattc tttcgatccc ctgcaccgac ggttcaggca ttatccctat
    27541 cccggaataa tatcctcagg ttacgccacg gtccattgca tcctcgagga cggtagtttc
    27601 ctttggctgg gcggcgataa ttatttattg caccgttttg acaaaaccac cggtcaattt
    27661 gaatctttcc ggccggcctg gatcagtcct gaaatagaaa atgaccggtt ttccctcacg
    27721 gaaatttttc aggaccgcct ggatccggac cggctgtgga tggccttgat caatcacaat
    27781 ccaaaatacg ccgggaaaaa agccacctgg ctgatatctt ttcataaagg caataagaca
    27841 tttcagagac acgaatgctg gggaagccca aggcatcagg atcccgacgg acggatctgg
    27901 ctgacctctg aggggataaa ccggtacgac ccggcctccg gtacctgcga gcatttcccg
    27961 gtattcctca atggtgaaaa acaggggcca gaagtctgga tcagggacct ggtgcggcat
    28021 cagggcagtt ttttattgtc ggctccccag gcccttttgc gttttcagga ccatggggaa
    28081 gttcgggaag tggcctatgg aaaggaattg gggaacatgg gagatctttt tgtggatggg
    28141 cagaaaaata cctggattgg tcgtacgaac ggcatttccg ttctgagggc ccaggaagaa
    28201 agtatccgtt atttttcgtt tagtcatttt ggattttcag ggaggatcta cccgggaaga
    28261 atggcatacc agccggactc caataagata ttcgtagtgg accatagtgt agacggaacc
    28321 ggccggaaaa tcatggtttt agacctggat accggcattt caagcttgtt ttatgaaagc
    28381 ccacacccga ttcatggaat cgtagtggat acttcaggcc aattgtggat gagcagcaac
    28441 ggtgcatttt atactttggc cgtccgcgga aacccttccc cgaagcctaa aagggaaaac
    28501 agtcatgcat cccctgttcc ctggctttgg aatttatccc tgagtccggg cgggtggatc
    28561 gccggtacgg gaatacatca attttggtgg ttcaagccgg gggagcctgc acgggtattg
    28621 aacctggatc agttggattc ggccggccag ggaaaatccg tatccaggtt gcaaggcttg
    28681 tccttcaccc ggcaagatga agccttgttg ttttccaacc ggattttttc ggttgattta
    28741 cagtctggga aaatcactcc cttgctgatg gaagaccatg taaacccttt tccgtatacc
    28801 gatatccaca gcgtgatgca agaccaccgg ggacatatct gggtaagcaa ccttgcaatg
    28861 accgcagaat ttaaaagatc cggagaccgt ttggacctgg tcaaaaaata ctctgccggg
    28921 gacgggctgg gtgcagcctg ggttcatgag attacggtcg acgagcgggg aaggatctgg
    28981 gctttcgctc agaatggact gcaggttata gatccggttt taaaggaagc ccggtcgttt
    29041 ggagtccggg aaggcctgga gaatccatac atcgatccac gacaggtcct gcatctcaag
    29101 gatggccgca tcgtgtcggt caacgggccg ggcattatcc tttttcatcc cgataccctg
    29161 tggaacctat atacccccag ggccgtcccc ctcgtcattc gcgagataag gattgccggg
    29221 cagaccctgc ccccggatac cgatttgaac ggcatcaaat ccctccgcct gaagcccgat
    29281 caaaattttt tggatgtgca gtttcaggca ctggcttatc ccacagattt taaaatgacc
    29341 tacagctaca ccgtggatgg cctgcatccg ggatggattt ctattggtga aaataaaatt
    29401 attaccctgc ccaccctgtc ccccggggat tatgtcctga agatcaaggc gggagaaccc
    29461 tcgagcccgg ctccggaaaa agaactttca atacatgtcg cggccccttt gattggccag
    29521 gcctggttca gggtattgtc tgttctgtgc gtcgcgggtt tgctggctgg attatttttt
    29581 tggcgtaccc gcaggatccg gttggaagaa gccgggaaag ccctggtgag caggcaaatg
    29641 gcggaactgg aaatgaaggc tttaagggcc cagatgaacc cgcattttat gttcaacagc
    29701 ctcaattcca ttaaaaatta catcttccgg gccgaaccca aaacggctgc gcaatacctg
    29761 tccaattttg cacacctgat ccggcttacc cttgaccata gccgggaaaa gcggatcacc
    29821 ctgaagcagg aattggatgc cctcttgctc tatatagaac tggaacagat gcggtttgaa
    29881 aaccgctttg aactggtctt cgatgttccc gaaacgctgg atctgcacag gctttatatc
    29941 ccgcccctgt tgctgcagcc ctttgtcgaa aacgcgatct ggcacgggat catgcataaa
    30001 caggggaagg ggcaccttcg ggttggtttg tcaaaatgca acggcgtcat tacctgtgtc
    30061 attgaggatg acggggtagg caggacccgg gcgggagaaa ttaaaaaact aaaccattca
    30121 aaatatcaaa gcatgggaat aagcatcatg gaggaccgga ttgagattat aaacaaactg
    30181 gaaatgcttc agatccggat agaaatcctt gataaactcg atgatttggg gaatccggcg
    30241 ggtacccggg tgaacatacg gatcccggaa ctttccgaag attaaattaa agccaggaac
    30301 aatgacgaaa ataaaaacca ttctggtgga cgatgaaaaa aacagcctgg aatccctggc
    30361 ctttgaaata ggggaatatt gcccggcggt agaaatcgtg gcggcctgcc aggacccttt
    30421 ccgtggcaag gaaatgatcg agaggcacca gcccgacctc atcttcctgg atgtcgagat
    30481 gcccggcatg aatggttttg aattgctgca gcagattccc ggattgtcga gccatgtggt
    30541 ctttatcacg gcgtatgacc agtttgcggt gaaggctttt gaattcaacg cggtggacta
    30601 tttgcttaaa cccgtgcgca tatcaaaatt gatttcctcg gttcagaagg tcctggaccg
    30661 ggtaaatcaa aaactggacc gggcagggct tgaggctttg gtccagaatt tgcaattaaa
    30721 aaaactgccc ggcattgaaa acattgcact tccgacccag gaaggattca agatggtgcg
    30781 cctgaacgaa atctcctacc tggaggccga aagcaattat acctgggtgg tcatggcaga
    30841 ccggaagaaa tatctggtga cccggacctt gaaggagatg gaagaccagt tgtcgcaagg
    30901 cccttacctc cgggtccata aaagctatct ggtcaacctg aacggcgtgg accgttatgt
    30961 gcgcggccag ggggggtatt tgatcatgaa ggaccagtcg caggtaccgg tggccaggac
    31021 acaaaaggag gttttgctga ggtcccttaa attataaacc gtcgaaacgg ccggcacgct
    31081 aaaccccggt ctatcttttg aaaatccgag ccagtcccct tcctttttct gtctttaatt
    31141 ccacgaaata gattcctgtg ggcaggtagt ccatcgggat cgtgatgacc tctcccccgg
    31201 ttttaaatga ttttataacc tgtccgctgg gcaggattaa actgacctgt tttgtatgga
    31261 agggttggtg aaaggcgacc tgaagggttt gccggctgcc ggtataaata attttaaaca
    31321 gcgaaggatc caactcagga atagcggtcg ttggaagggc gaggtggaag gtggcgcccc
    31381 ggatgggatt ttcaggtaaa aaccaggggt gactggcccg gaaagaataa cctcctgcct
    31441 gggccggatt aaaggattct atcgtgtatt cggcaccgtt cgctcccgga atggaattac
    31501 cgttccgaaa ccaggtgtat tgaatttcct ggtttttcat aggggaacta agaaccactt
    31561 tggaaccggc ttgtatgacc aggatcgtgt ccttcccgat caggccctga ggataccacc
    31621 ataacttgtg tgcaggcagg ccggcattct gggccaggtc gtcaaagtcc agccggttgt
    31681 tgataatcac gacacgaaga aagtttggat tcgggtccca gttggaaaag tttcccagct
    31741 ccgtgaggag gttgtctgaa aaatccagta tgaccgcttt ttgaggatta aaatgggccg
    31801 gggacaataa gccggtaaag gcgttcccgc ttccttcgca gtaaagcatt tcgggataat
    31861 ggcccaatag ggtatccagg ttgcccgata gtttattatt gttgaaattc atggaactca
    31921 aatgaaccag ggagtccagt tttggcatgc tgccttcaaa gctgttgttt gaaaaggcga
    31981 ggctttccag ctggaccagt ttattcagcc aggacggcag ggctccgctg aatttgttgt
    32041 cgctgatatc caaactaagc aggccgggca tttccgttaa cacttcaggg aagggtccgg
    32101 aaaggttgtt tttattcaga tgcaggttac gcaggctctt taattgggcc aggctcgatg
    32161 ggagtcctcc ctccagcctg ttttcaccca ggtataattg gaccaactgg gtgcaggacc
    32221 ccaatgaatc cgggataagc ccctgaatgc cgcttcgcct tagattgaga acattcaatt
    32281 cggtaagccg gccaagggaa tgcggaatgg gtccttcaag gtcggacatg cccaggctca
    32341 gttcatgcaa ggccgtcaga tggccgattt caggaggaat ggagccggat aagccattgt
    32401 tccacaaatt gagctggacc acccggttcc cctgggtagt tacgccatac cagccggaaa
    32461 cgggtccatt caaccaattg tccttattgg tccattgggg gccggacgtg gactggtaaa
    32521 gggcaaccag cgcgacggaa tcctggataa ggacctggcc ggtcaagggg aagatcaggc
    32581 aggctaagca cagaattgtg aaataatatc gtctcatata tgttttgacg taaaataatt
    32641 catttaaaac ggtaaatcca tccgtattgc gcgtgcggcc ggattgttga gagaacggtt
    32701 cgatacgcgg ggcgaacgga tatgccgacc cttccgggca gggaaatgga atagccctaa
    32761 gctgcattga ccactcaatt tttaaacaaa ccgtttgcag cttacccttt aaaccagtct
    32821 ctttacaaaa tatatggggc cgttcacgca tttatagtat atgaaccgga taatctaatg
    32881 gagatttaat cgggaaagga aagttcaggg accatccggt ttggtgtaat gatcttttaa
    32941 cctttaaaga ataaattaac atgaaatatt ccatcctctt tatagccttg agtttttgga
    33001 tcacctgcac caaggacaac gtacagaccg atcagtcgat tgacggaacg ttcttggaag
    33061 aacggggccg caaagtaaaa atcagcctct gccatattga tgaaaacgga gatttccagg
    33121 ccctgtcggt acctcaaaat gccgtgcagg cccatcttga tcatggagac tatctggccg
    33181 atgccgacgg ggatggatat tcggcagtcg gggcctgttc cggcagcggg gatgattgtg
    33241 atgacgagga cccggatata aacccgggaa gcggggcatg caccggttgc gtatgcagtg
    33301 acctggatga attgaatgac agtaacctgc tcttttattt taattcagac aacaacccct
    33361 gtcatattta taatgatccc accggagtca ttctgttttt tgaggcctcg ggagacacga
    33421 tggtggcgct tgccggcatc gaagaagatg gccaggtcat tgtcggcctg ggagaagtgc
    33481 aaaatggatt tatacaagaa tcggaacatg gatgtaagca ggttgtaggt atggacatca
    33541 ctctggaaca atatgagact tgcctggcag gattgcgggc catcattgca agtcacccta
    33601 cattgcccaa tatctgcgac atcgacatgc ccccaatgtt tatcgacacc aacccgcagt
    33661 aggggcataa agaagagagt caatccgctc ataaaaatga atatcccgga ctgaccagtc
    33721 aggaatccgg atttcaggta ctcaggcatt aaaaaattgg actatgaaaa aattcaaata
    33781 ctcatttatt tgtgttttac tctgttttca gccgtcctgg caaggttcct tttttgccca
    33841 ggacctgtgt gatacggtcg tatccgcctg gggcatgcat gagttcaccc tggagtccgg
    33901 aatttcaagg atttcctttg tggccttggg cgcggacggg ggaaatgcag cagtgaggaa
    33961 tacggatata ttcggcaacg tgaccacctg tacttccatg ggcggctccg gggcaagagt
    34021 gcaggcgacc ttcaatattg ggacaggcac gaatgaaatc ccagccggtt ccacgatacg
    34081 gctgattgtt ggccaggcgg gcgaaaacca tatatccgat atttcattcg gaacaggtta
    34141 tgcctatggg ggaggcgggg gtggttcagc agtgctttat cgggctcccg gctctctggt
    34201 ttggactacc ttattggccg cgggaggcgg gggtggtgcc tatcagggga tgtttgcaaa
    34261 tgcctgtatt gataatgaaa atggccaggg gggccggtcc ggcacaagtg ggggcaatgg
    34321 caccggggat attggtccgg gtagcggagg cttttcaggt accagtggag atggcggtgg
    34381 ggcgagtgga ttgatttctg ccggaggagg aggtgcattt tcggatggcg gaggtatccc
    34441 ttgtgatggt ttcaacgggg aagggaaagc cggattgccg gcgggaggag caggagggac
    34501 ccaggagaca tgtttcttgg gagaactttt taaatatggg ggctttggtt ttggcggggg
    34561 cggagccgga agaggcgctg gcgggggcgg aggcggatat gccggtggag gcggaggcgg
    34621 tactacagga agcggcgggg gcggaggtag cggagtggat cccagggcaa tcagccaggg
    34681 aatcacggcg ggtggtaatg acccggcttc tttggacggt tcgatcgcga tcagttgttc
    34741 gcttgcgccc attgcaatat gtaaaaatgt caccctggat atcgaagaag gagacacagt
    34801 attcgtcacc atttccgata tagacgacgg cagttatgac cctggaggag gcgccctgga
    34861 aaaagtactc ttttctcctc cctttacgtg tgactcggcg ggcactcgtt ttgtcaccct
    34921 gacggtgtac aatgaagagg gcttgtccag tgaatgtatg gcccaggtca cgatccttga
    34981 aatgactccc ccgaccatac attgtcagga tccaatccgt gttgatgtat ccggctcggg
    35041 tttgtgtggc gcctatgttc catactatat ggcatgtacg gattgcagct cctgtaccat
    35101 tctgcaaatc gaaggcctcc ccaatggagc ttttttcccg gtgggtacga ccatcaactc
    35161 ttttgtggct attgatgcct cgggtaatcg ttcggacacc tgcagtttta cggtcaccgt
    35221 tgtcgatgca gaaatgccga ctgcggtttg tttatccttt gtggaggttt caccggacac
    35281 atcgggtaat tatatcccgg acccggccct gatcaattat ggaagttatg acaattgtgg
    35341 cataggttca ttgaccgttt ttccaagcct gatcccttgt gcgcacgaga gccctaaaca
    35401 aatggtgacc ctgatcgtca aggacctcta cggcaatcaa aatacgtgca gctctgaact
    35461 caccctcctg gatgatgacg attacgatgg agtcggaaat gattgtgatc aatgtccggg
    35521 cggtgacgat caggtggaca acgatcatga cgggcaagcc gattgtgccg tcttccccgg
    35581 tatggaccat ctgatcgatt catggaaatg cggcaataac agcaataagg ttttgatctg
    35641 ccatattgaa tcgctggaac catttgtatc caccccgctt tgtgtccacc cgaatgcagt
    35701 ggcggcgcat ttggctcacg gggatttcgt cggtccttgt cctggcccga tggaggccgg
    35761 cagcaaaaag gattctccgt cgggtccaat ccattcggaa atcaataaag gttccgggac
    35821 gatgatcgct tcaagggatt ggtccccggt ctaccccaat cccaattccg gaaacttcag
    35881 gatccgcctg aatggcctcg aaatttcaga aaatgctccg gccacgttct ccgtttattc
    35941 cacgctgggt aaaaaaatat gggaggaaaa aacttttgtt ggagcgttgc aaaccctcca
    36001 ggtggatctg catgaatccc tgcccccgga tgggatctat ctcctgcata taaggaatgg
    36061 agaaaagagc atcgtgaaaa agttccagat cggaagacct taaagattac ctgaccggat
    36121 gaactggaac gatcctgcct catgaatccg agacgggaag ggctggaaaa atttcattta
    36181 tccatgatgg atttatttct cgactgccgg taaaggccgt acaaggatcc ccggtcaagc
    36241 cggtataaat aaatcatggc aaaggcgcac atcgccccgg atatataaaa catgtaagga
    36301 atacgatcga tatgagcccc ggttaaaaat cctgcaaaca gggctccccc cccaatgccg
    36361 atttccaagg cgatatacat ggtcgccagg gctctgcctc tcttggcggg atcgcccagg
    36421 tcaatggtcc aagcgctgat ggttggaaaa tatattccga cccccaggcc aaaaaggatg
    36481 gaagcccagt aaaaccaggc cggggaaaca ctgtgggcaa aaaccagcat cgacaggatc
    36541 ataatatata tggaaaactt aagtaccggt accctgccga tccggtcagc aatccggccg
    36601 gcaaaaaacc gtgttgaaat actggacagg gtgaaaaggg taaaataaac gccccggttg
    36661 cctgcctgga ggcggtcact caggtccggc gccagcgtca acagcacccc gtatgaaaaa
    36721 taacaacaga tcatgacgat ggcggcgggc agggccagcg gatcaaagag gtcttctttt
    36781 tttactttca gatgactgaa acgaaacgat tcctttccac ccagggtttc gggaagattg
    36841 atcaggatca gcatggagag cagggccagg aaggatgaaa aataaaacat gatattgata
    36901 ccaaatgcct ggaccatcca gctgcctatc ggaggagtgg cggatgaacc gacactcgat
    36961 gagatcccca tgattcccat agcctccccc ctgcgattcc agggtacgat atcggctacg
    37021 taagccgctg aagcggtcgg cttgaagccg gtggaaaaac catggatcaa acggataaat
    37081 aaaaaacccg ctaccgtgtg caccagagga tagaggaccc cgcaggcaat acaggcgaat
    37141 accccgaaat acatgaccgg gatccggccg atggtatccg tcagtttacc gctgaagggg
    37201 cgggaaacgc ctgccgtaaa ggtgaataaa gccaaaatca agccgatata atgcgctcct
    37261 cccatggatc tcaggtaatt gggcagctcg ggcaatatca tattgaagct gcccgcgaaa
    37321 agggcatggg acaaacaaag catcagaaag ggagtggaat acagcctgct ggtcacagga
    37381 ggggtttgca tcgggcgaag atagcgaatg gaaatgcccc atagtcctgc ttccctgaat
    37441 cctggtccgg gcgctcatct gtttgcctga tcccattcat cagaaggaac agatgtatgg
    37501 tatattgaat ttctaaatat ataacggcga tgggatccaa cttcccctgg cccatgttcg
    37561 aatttttatt ttagctgttt tcagttctat gccaggggga taagcttcgg gaggtcgatg
    37621 gcggaaaggt ttgctgcagg aatagaaaag aatgaaaaaa aataatatat gataaagttt
    37681 catttttggg ttttaccctt gggattaatt cttattttaa tttcaaattt tgaatggcta
    37741 tgaaaaagta tcttctattg accggggtta ttttattatg catccctgta ttgaattcgc
    37801 agaactgcaa tctgatctgc ctctcgaagg tgaatgtatc cctaagccct gcaaacggtt
    37861 gcacggccaa tccctccccg ctgaatttca tcagcaatcc ttcccccacc ccttcgcctt
    37921 cctgtgcggc ttaccagctc aaaatctcct atccctatgg gacgagcaag tacaatccgc
    37981 cgacaaaatt ggataaatcc caccagggct ataccatgct ttaccaggtg cttgactcgg
    38041 tttccaggaa tgcctgctgg ggttacctca cggtcaataa atgcaatccc tgtatggtac
    38101 ccaccccgcc ggtggccgat accctgattg cctcgcccat gtccgggcct tatcctgcag
    38161 ggtcgatgat taccctcacc gcaacagcta cggataatgt ttcgattgcc aaagtggagt
    38221 tttatgacgg gctaatatta ttgaataccg atctcaccgc tccctattct ttcaaggtga
    38281 ccacttttgc cttatccaaa atgtatcagt tcaaggcggt cgtttatgac aattgcgggg
    38341 ctagggatac gtccaatatc gtactgatca ccacggatcc acccacctgt acggacggca
    38401 ttaagaacgg atttgaaacc ggagtggatt gcggaggcgg cggatgcagc atttgcctcc
    38461 ctccgcccgc cccaccggcc tgtatgaccc ccgtcctgct gccttccggc tcattggata
    38521 cggtcaggca atcctcgacc tcgtctgctt attatccggc ctccaaggcg agggatgcct
    38581 cattgtatac ctacagccgg accaatgccg aactccggcc ctggtgggag atcaacctgg
    38641 gagccaccta taatatttcc agtctggaaa tcaggcaccg aatctgttat ggatgcaaca
    38701 attatttgat ccgatacaac ctgtttatat cccccactcc catcaccact acagacatca
    38761 acgtgcttaa agcggacccc ctggtttata aactgcccta tgtggcatcc agttttaccc
    38821 tcaatggggt taatttcctg gggagataca tccgcatctg ggcggaatac agcagtccga
    38881 acagcctcag cctgctggat gtaaaagtcc ggggttgcca ggtcatgatg gttgcctctt
    38941 ccatttccgg caaactttcc gaccagggat ccctctccgt cctttcaacg gatttgcaaa
    39001 gcctgactcc accggaaggg aaacccatca attggcagga ggcatccgat acggatccaa
    39061 aaccaagccc catcaccgtt ttcccgaatc ccgcccgcga ccggatattc ctttccatcc
    39121 ccggcggata tgttcaggcc gaagccctga tcacagacat tctgggcaga aaagtgatgc
    39181 ggcgcccgct ggcgggaacg gaactggaca tcagtaccct ggcccagggt cattacctcc
    39241 tgcagctcca ggtggatgga gttaagaccc tgcaccggtt tatcagggaa taagagcgat
    39301 taatagttct gtaaatgaaa aagggccgcc tgcttcaggc agcccttttt aggaaatatt
    39361 caccgggatt ttttacttgg ttactccagg attgccgttc tgaaagctca aaagctcgga
    39421 atctccgcac tcgcctggag cttttagcta aaatgttcat ggaggacttt caatataata
    39481 tttttcaatg gtgttattat tttgtctaaa tcatgtgcga attaatatac cggtaaatga
    39541 aaaagggccg cctgcttcag gaagcccttt ttaggaaata ttcactggga ttttttactt
    39601 gtaatccaat gcaggtcggt ctgctgcatg gaatattcgc tgatattaaa gtgcggcaat
    39661 cttatatttt atgactggga agcggacaaa aaaagaaggg atgaccgtca tggacatccc
    39721 ttcgtattct cataccggca tttaatagag atagtccagg gctgttttgt catcgttgtt
    39781 gaaaggacgg ttctgacctg aaccaataca ggacaacatc caggactggg cggcaagggt
    39841 cgcatccgtg ggagtgcccg ggatatggat ggcccccaca ctgctgctgc cttcattgac
    39901 ggcgcttcct ccgcaactga tcgaccgatc gtaaaaatcc gtatgcctga aaccaatgca
    39961 atgccccagt tcgtgggcga ggacggaagc aatggtgccc agcggctggc cggacagcgc
    40021 tccaaggttt acagacaccg aacggtatgg attcccgttg cttgggaaac cggctgatgc
    40081 cagatagcct gcactaggag aggatttgac gatcttgata taaccgtttt tgctcacccg
    40141 ctggaatttc agggtaagat tttcggcgtt ataccgggcg atcgcctcat ccagggcggc
    40201 agaaatggtg gacaatttgc tgtccagact tacactgatg gtacgggtgc cattgaccgt
    40261 caccaggttg gtggtccggt attgctcaag gatcggcaac ctttgattgg gaagcgaaac
    40321 caggtcctga tcggtcagga aaatatcccc ttccacgata taccccccct ccgttttgta
    40381 ggagtctttg ggattaaaac cgaggttgat gatcttggcc ttgatctcgg ccgtgatccc
    40441 ggtaagctct tcctcagagg tcttgatctg agattcctgc tggcaggaca ggataaatag
    40501 tgcaaggaga agaattgtaa aatttagcag cttcataatg aataagttta agagttaaaa
    40561 attggactat aaattggtaa aagtttcaaa tcatggatac ccgactgaaa gcggcggaaa
    40621 tggtttagga tgggtacggg ggtacaccat cttttaaagg aggatattcc cggacaccgt
    40681 ataaatcagg gtctggctta atccctccgg tttgatccgg ggaaattcac taaagaaaat
    40741 gaaagatttg catttcccac tgggtgattc ttaccaatca accggccaat cagtcaaagc
    40801 gaatgtaaag ggcccttgcg ggaatttttg aactcagggt ggacttgaag cgagccaatt
    40861 aaacaggaac ctcttaatac ccttcggaaa ggaagttatg tattaaaaag tccgattttt
    40921 gaccaatgta gtggaatcca ccagtattcg caatcggttt tttatatata ttttttttag
    40981 atattttcct tagagaaatt caggccctca gcctcttccc tggcccgatc tgccgatctg
    41041 ttttatattg aacgatttgg tttaaatttg aacggaatca aggtaattga atgtgaaacg
    41101 tggactgcag cttttcgccc tgtataaaaa gaactgcctt ctttccacca tcctgtttgt
    41161 cctgggcctg caatctcagg acctgatcgc ccaattccgg tttccgccgg aaatttcccg
    41221 catgtccttt ccggcttttc ctcaaaccga gttggtcatc aaccggcttg accaccaggt
    41281 caattatatc cgtgccgccc atttctattt cccggatctc cggggggagg gcatccggat
    41341 ttcggtaaaa gaatatctgt tcgatacttc agatatcgac ctggcctatg cttaccggtt
    41401 ttcaccccac caactcccgg tcattcaaac gcattccacc atcatggcaa ccatgatcgc
    41461 cggaagaggc aactcatccc cttcgtccgt tggagtggca cctgccgccg ggctgtattc
    41521 ttccagcttt gtcccagcct tgccggacac cgataattat tacagggcca acgccatcag
    41581 tgttcaaaat cattcttatg gagaagacat caataatgtc tatggtccgg aagcggccgc
    41641 ctttgaccaa aatgcctggc aaaacccctt tcttctccac atcttttcca gcggcaacag
    41701 gggaaacgca gccagcccgt ctggcaggta ttcgaacctg gaaggcttcg ccaatctgac
    41761 gggcaatttt aaaatgtcca aaaactccat tgccgtagga gctattgata cggcggaccg
    41821 gatcgaaacg ctcagttcga ggggccccgc ttacgatgga cgcctaaaac cggaactgac
    41881 tgcatttggc atcgagggca cttccggttc ggccgccctg acttcaggct tggccgcttt
    41941 gcttcagcag gcttacaaaa aatatcatga cgacagcctc cctccctcat ctctgatcaa
    42001 ggcagctctg atcaacagcg cccttgacct gggaaccccg ggacctgatt ttacctatgg
    42061 ttatggaaaa ataaatgcct tgcaggcttt aaaaacgatg accgggcaaa aatatataat
    42121 aggactgctc aaaaaggatc aaaccgccag gcatgctatt tccatacccg ctgacgtaaa
    42181 aaatcttaaa atcaccctgg cctggttgga aaaaccggcc gaaccctttg ccccctttgc
    42241 cctgagaaac gaccttgacc taagggtgga ggaaccctcc acggcacttg cccatctgcc
    42301 ttatgtaccc aatacctttc ctcatcccga ttccctgacc ttgcctgcaa gtcccgggga
    42361 ggaccatctg aatacggtgg aacaggtcat gatcgcggat cctgggcccg gaacctatac
    42421 aataatattg ggagggggga tgatggattc ctcccaggct tattccttat gttatgaatg
    42481 ggaagaaagg aaccggctga cctggaccca tccactaaat attgatttcc tcgaggcagg
    42541 tcgttctcaa gaactgaggt ggctttccac ctatgatccc aaggtggaag gaagtcttgc
    42601 cttcagcttc gacagcctga cctggatgcc catccttacc ggcctgagcc tgtcagaggg
    42661 aaaaacaaac tggctggtcc ccgataccaa tgcggtggtt tatttccgat tgactgcgga
    42721 tggtgcggag tacttcaccc gttccgtgat cttgaagaaa ctggaaatgg aagtgggata
    42781 tatctgcgaa gattccgtac tggtatcctg gaataaactg gaaggcgtgg attcctttgg
    42841 catttacaga atggaccagg gtctgatgcg aaccagcggc cataccccgg atacgttttg
    42901 gatattcaag gcggatccgg gctctcccta tattgcggtt tccaccctta tagcaggtca
    42961 tgaaacccgt ctgcccgtcc tcaattacac cgaccagggg gtggcctgtt ttattcggca
    43021 atttatcggg cggaagatcg acgaatccaa tgcggaactg accctggcgc tcggaacggt
    43081 ttttgggata aaaaatatcc ggattgaccg gatcaatcac cgggatagcc ttactgtact
    43141 caatgccaac cttcccaccc aggcaaattt tacggttgaa gccggattaa aagaagggtc
    43201 caatctgttc aggctcagac ttacccttca aaaccagcgg accgtcacgg atgaagcccg
    43261 cctgtacagc ctgggcagcc gcattggcag cgtcttcccc aacccggtcc gtggagggca
    43321 atctcttaac cttaagaact acaaaaccga tcccgtatcg gtgaccctgt ttgatctcaa
    43381 cggaaggccc ctcttcaata ccctgctgcg gggggagggt tcccttgacc tgcccggtct
    43441 ttccccgggg atttatttta tcgggtttta tgagaacgaa aagctccggt tcagagaaaa
    43501 ggtggtcatt tattaagtgg tcatttattt aatccatgtc tggtcgggtg aaaatgacct
    43561 cgtggggtga ccttttagcg aaaatgggat gtccgggttc tgaaaggcca ggcaaagtcg
    43621 tcaggcagac tttgggtccg gctttcaaag ctcaccgctt ttccgcatct ttgcgcccca
    43681 attccaatca atatggcagg tgaaaagatc attttttcga tggagggggt taataaagtg
    43741 tatcccccga acaagcaggt cctaaaaaat atatacctca gtttttatta cggggccaaa
    43801 atcggcgtgc tcggcctcaa cggctccggc aaatccagtc ttctccgcat catttccgga
    43861 aaagaccaga attaccaggg caagataatc tttgataagg attataaaat cgggcatctc
    43921 gagcaagaac cggtcctgga tccggaaaag accgtgaggc aaacggtaga agaaggcgtc
    43981 ggcgccctgg tcgccctaat gaaagaatac gaggaggtat gcaacaagct ggctgagccg
    44041 ctggaagata atgaaatgaa ccgcctgatt gaaaaacagg cggaactcat ggaccgcctg
    44101 gaccaggaaa atgcctggga actggaccat aagttgaata cggccatgga agccctgcga
    44161 tgccccgaag aaaaccaaat catcaaggtc ctttcgggcg gagaaaaaag gagggtcgcc
    44221 ctttgccgct tgttgctgag caacccggat atccttttgc tggatgaacc caccaaccac
    44281 ctggacgcag aaagtgtgct ctggctggaa caatatcttc agaattttcc gggtacggtc
    44341 atcgcggtta cccacgaccg ttatttcctg gacaatgtgg ccggatggat cctcgaactt
    44401 gaccgggggg aaggcatacc atggaaaggc aattattcca gctggctcga acaaaaggcc
    44461 aagcgtctgg aaatggagga gaaacaggaa accaaaagga aaaaaacctt ggaaagggaa
    44521 ctggaatggg tccggatgtc ccccaaagcc cgccaatcca aaggaaaggc ccgactcaat
    44581 gcctatgaca agttgctgag cgaagacatc aaagaaaagg aagccacgct cgaactttat
    44641 attcctcccg gcccgcggct gggggacgtc gtcattgaag cccaggggct ttccaaagca
    44701 tttggcgacc gggtcctttt tgaaaacctc agtttctcca ttcccaaaaa cggggtggtg
    44761 ggcattatcg gccctaacgg cgtggggaaa agtactttgt ttaagatcat catgggccag
    44821 gaaaaaccag acaccggtaa ggtgaccatc ggtgacacgg tacacctttc ttatgtggac
    44881 cagactcaca aggacctgaa tcccgaaaag accatttacg acgtgatcag cgggggaacc
    44941 gacattattc ttgttggaaa aacggaaatc aacgcccggt cctatctggc acgctttaat
    45001 ttcacggggg gggatcaaca gaaaaaagta ggggtcttgt ccggcgggga aagaaaccgg
    45061 ctgcatttgg ccatgaccct caaggatgga ggcaacgtac tcctcctgga tgagccgacc
    45121 aacgacatcg acgtcaatac gctaagggcc ctggaagagg ccatcgaaca ttttgccggg
    45181 gccgtcctgg tgatctccca cgaccgctgg tttattgacc ggctggccac ccatatcctt
    45241 tcctttgagg acgacggcca ggtggtttat tttgaaggaa acttcagcga ttatgaagct
    45301 aagaaaaagg agaggctcgg agacctgacg cccagcaggc cgaggtttaa gaatgtgatc
    45361 aacaggtaaa aggcgaatgg cgaaaggcga agggtaaaag gaaagtaagc taatgcgctc
    45421 acactccacc cctcatacta caacggttta atcttaatat tcctgaacca caccgtattt
    45481 ccatgatcct gcagggaaat cttgcctttg gtaaaagcgc cgaagccggg catgtctttg
    45541 aatttgcttc cggccaccag ttttttccag gcggggtcgc cgaccctggt gcttaccact
    45601 ttttcaccgt ttaaaaaaag atcgatcgct ccgttctggg aaaggatctc cgcctggttc
    45661 cattcaagcg catgggtcac gggctctttg gaacaagaga tcaggtcata gagatcgccg
    45721 gcccggtgct ttttgatttt ggcatcgcgg tggcaggcat tgtccaggac ctgcatttca
    45781 gggccggtat gccatacgta agggtatttg gccgtgtctt cgtttacata aaagataatc
    45841 ccgctgttgc cgcaggtatc gattttccat tccagcttga ggtggaagtt ttcaaactct
    45901 tcgtcggtga caatatcgcc cccgccctgg gtttgccagt cggctttatt cgaagcatcg
    45961 agcatgagcg tgccatccac ggttttccag gccggtccgg gggcgccccc gccatattta
    46021 tgccatccgg tggtggatat tccgtcgaac aagggcctcc atccgttttg ggtctcttcc
    46081 ggagtaagga ctactctgtt tggattggtg gcggttgggg ctgtggaagg ctccattgcc
    46141 ggagtgcttt ccttttcctt gcctttacag gcgaacagga tcaggcctaa gagcagggcc
    46201 agggatgtat atttcatggt taaagccggt ttattttgtg acgcccaaaa gtagaaatta
    46261 tttttaacaa gtgtagtggg ctgttaatca atctccatga tccggtttac cagaaaggcc
    46321 ggtgaagtcc aagtgaaaac caaatccagg gatgacttgc tctatggtgt acgatccgga
    46381 aaattcctga tactgaggat tttctgcggc cgggcatctt gactgcaact catttatagt
    46441 gtaccttgcg tgttttgact ccatttactt acctataata gttttgatta tgtcaggaaa
    46501 cgacaattat agcttattga tagataaatt ggatcaattt atccgcaaat actacatcaa
    46561 ccagatcatt cgcggggttt tgtttacgac cgggtctgtt cttttgttgt tcctggccta
    46621 taacctgatg gagcatgaat tttatttcag caaaggggtg cgcaagggat tgttttattc
    46681 ctttaatcta ttcagtgctg gcgccctggc atattgggta ggcgtccccc tgatgcatta
    46741 tttcaaattg gggcagatca tcagctatga gcaggccgcc cggatcattg gcgatcattt
    46801 ttcggacgtc aaggacaagt tgctgaatat ccttcaactc cggtcgcagt tgcaaagcct
    46861 ggaacaaagc caactgatcg aagccagcat ccagcagaaa agcgcccaga tccagatcgt
    46921 gccttttaaa aatgccattg atctgaataa gaaccggaaa tacctgaggt tcgccctgcc
    46981 tcctttatta ctgttgctgg tcctccttct cgcggcgccc agtgtgatcc gggacagctc
    47041 caggaggatc atacggaata atgaggaatt tgaaaaagca gcccccttcc attttaaatt
    47101 aaagaatgaa aacccctcgg tggttcagtt tgacaattat acgctggatc tcgaagtgga
    47161 cggcatggtg ctgcctgccg aagtttttat agaagtggac caatatcctt accggatgac
    47221 ccgggtggac gccaatcatt atacctatac gttcaacaac gttcagaaaa ataccggctt
    47281 cacgatgttc agcggggagg tcaaatccaa aggttatgaa ctggcggttt taaagaaacc
    47341 catcctcagc aattttacca ttcggatgga ttacccctcc tatattggcc gcaaagccga
    47401 gtccctggag aacatcgggg acttgctggt gcccgaaggc acccgtcttg cctggcagat
    47461 cgatgctgaa aacacggaca aggttgccat ggctttcggc tcagggacgg tccaggagat
    47521 cagccggaac ggggaacgtt attttacgca ccggcaccgg gccatgcagg accagcctta
    47581 taagattttt atctcaaacc aatgggttcc cgttccggat tccatcctct accagctccg
    47641 ggtggtggcc gatcaatacc ccagcatcca gctcgaacag tttgtcgaca gcctggataa
    47701 acgattaatc tattttgccg ggaacggctc ggatgattac gggttgtcca aactcacttt
    47761 taattattcc gttatccatc ctgacggtaa gcaggtcaat gcagttccgg tggccatacc
    47821 catttccaat tccaacaaaa cgacctacca gtatgaatgg gacagccgga acatcgaact
    47881 gaaaccgggt gatcagctca gttattattt tgaacttttt gacaacgacg gaatccatgg
    47941 cgccaaatcg gtcaaatcca acgtcctttc cctgcgcatg ccttccgaag aggaactgga
    48001 aaatcaaaac agcaaaaaca acgatgccat ccgggatgaa ctcagacaag ccctcgaaca
    48061 gagccgcagc ctccagcaac agatggatca gctgagggaa aaaatgatcc aggagaagaa
    48121 acccaactgg caactcaaaa aagagcttga aaaattgctg gaaaaacaaa aagaaatcca
    48181 gaaaaaaatc gaggaggccc gtaaaaagtt tgaagagaac ctgaagaacc aggaattgtt
    48241 tgaagaaact tcggaaaaaa tactggaaaa acaggaacag attgaaaaga tgttcgaaca
    48301 actgcaggat cccaaattgc aggaactgat ggaaaaaatc caagaactcc tccagcaaat
    48361 ggaaaaggac cagactctcc cgatgatgga agaaatgaat atcgaaaacc aggagctgga
    48421 aaaagaactg gaccgcatga tggaattgtt caaaaccctg gaggtcgagc accagatgca
    48481 aatggccatt gataaactca gggaaatggc taaagaagaa gaacaactca gcgaagaaac
    48541 ccggatgaag gcggaggaag aaaagcagcc tcaaaaccag gaaaatccga ataaggaccc
    48601 acaggataat aagggagaac aaaagaattc cgatcagaag aacaaacagg accaaccggt
    48661 taaagaagac ccccagaaca agtccaacga tgaaaataaa gcgggccagg acaaccagga
    48721 aaaagaccaa aaaggagaac aggaaaagca gggagcggat caaaagcaac cggaatcgaa
    48781 atcacaggaa gagcttcaga agcaacagga agagctgaat gagaaaatgg aaaaactgca
    48841 ggaagaaatg aaggaactgg aaaagaaaaa cgaagacctg cagcggccaa aacaaatggg
    48901 cgaaccccag aagaagatgg aggatatcaa acaggagatg cagaacagtt ccaaacaaat
    48961 gcagcaaaag caaaacaaca aagcctccaa atcccagaaa aacgccgccc agaaaatgaa
    49021 agacatggcc cagtccatgg aaaaccagat gcagcaggag gaacaggaac aggcccagga
    49081 ggatgtgaaa gccctccgtc agttgctgga aaacctggtc accatgtctt ttgaccagga
    49141 aggcctgatc aaagaggtga ataagaccat gctcaatacg ccccggtata ccgaattgct
    49201 gcaacagcag tttaagatca aagaagattt tagcctgatt gaagacagcc tccaggccct
    49261 cagtaaaagg gttttccagc tcgaagcctt tgttaccgaa aaagtcaatg aaattaaaac
    49321 ccagatgggc agtgccctcg acgagctgga agaaaggaat aaagccaaag ccggggatca
    49381 ccagcaaagg accatgaaga atgtgaatga cctggccctg atgctttcgg aatccatgga
    49441 gaacatgcag gcccaggcct caggggcagc ctgtgccaat cccaaaaaat ccggaaaggg
    49501 agagggccag aagcccatgg ataagatcac ggagggccag aagggcatga aggaacagct
    49561 ggaacagatg aaaaaagcca tggaagaagg aaagggccag ggcagtaagg aatttgccaa
    49621 agctgccgca cgacaggcgg ccttgaggga agcactccgc gaactccaga aggaaaaaca
    49681 acaaaggggt gaaaacaccc aggaattgca aaaagccatc gaaggcatgg ataagacgga
    49741 aacggagctc gtcaataaaa aactgaccaa cgaaatgatg cagcggcaaa atgaaatcct
    49801 gacccgcctg ctggaagccg aaaatgcgga acgggagcgt aagcaggatg aaaaaagaaa
    49861 atcagagtcg gcgcaggaat tggaacgaaa attgcctcca tccatgcagg agtatttaaa
    49921 aaaacgcgaa gcagagatcg atttgtataa aaccgtttcc cccgatgtta aaccctatta
    49981 tcggtcgctg atcgaagagt atatgaaatc tctccggaca gaacatggaa atacaatcaa
    50041 taagtgaacc cggtacgcgt ccattgatga atacgtattg gaggttcccc tctacaccag
    50101 ggtgcattac ggatattgaa aaactggttg aatacctggc ggataaatat cagatttccg
    50161 cggagaagta tccgaatatc ctgatcagcc tcaccgaggc ggtaaacaat gcagtcatcc
    50221 acggcaatca ctgtgatgaa tgcaaatgcg tccatgtgga agtggaagtc catccccaaa
    50281 gcctgaccct gaccgtacgg gatgaaggct gtggatttga ccccgccaat gtggaagatc
    50341 ccaccctccc ggaaaacatc gcaaaagccg ggggcagggg gatattcctg atacaccagt
    50401 tgtgcgaccg cgtccgcttt gacgccaatg ggacaacggt aaaaatgagt tttgattttt
    50461 aagcattcca tacattctta tttccaggac cggacatgat ccgatttcat taccggtatc
    50521 cggccatgcg ggtgcgggag gcgggccggc tgaaatcctg gttggctggg gtggccaaaa
    50581 acgaagggaa atccgtctcc tctatggact atattttcgt ggacgaccct gtcctcctcg
    50641 aaatcaaccg gcaaagcctg catcacgatt atttcaccga catcatcacc tttccttatc
    50701 aatcgaaacc gatcataggg gagatttata tcagtgtgga ccggatcagg gagcatgccg
    50761 cggagtatgg ggtaagtttc tcccaggagt tgcaccgggt gatggtccac ggtttgcttc
    50821 acctttgcgg gtattccgac aagtccggaa aggagaaaaa gcggatgacg gagaaggaag
    50881 atttttatct ggaccaagtc tgagaccgga cccagaagcc gaagcattca gggaatcatt
    50941 tttccagggg caatttctcc ggaggatata atcagtgccg ttcagtaccg acaggagggc
    51001 tcgtgttttt taaaaatatt ttctgactcc cagcggcatt gggattcgcg gtcccgttga
    51061 aattttcgat ggacaagccg gttatattct cacattgaat cccgtgggta aaaaaggacg
    51121 gaaggtcgtc cgcccattgg acttcaaagt cgcggatgag gagttggttt acatattgcg
    51181 catataggcc cgggatctcg tgttcaaaca attgcatgga gatgggggtg gccggccgaa
    51241 ggtcaaaatt gccgccgtag tccatggttt cttttccttt tctcatcctc aattgcacct
    51301 ggttgaagcg gatattttcc aggcggcttt cttccaggcc ataaaccagg ataccttgtt
    51361 cccctgtagc attgatctgg ttgaactgca cgtttttgat ttgccctgcc cggactcctt
    51421 caaacctcga tatgcaggaa agatggatgg gttcgccatg cccccaccac tgtccgttgt
    51481 aaagccgggt ctcgatgatg atgttggtga atataaggtc ctcgatattg cttttatcat
    51541 gggcaaagat gccaatgccg cggtgcgaat cctggatgat gatgttggaa aacacccctc
    51601 tccggatggg atgctggcca tatccgatgc ggatgccagc cgaactggat ttgaggcggc
    51661 aattggaaac cgcaatattt tcggcatagg gcgatttatt gccgtaacga tgggattcct
    51721 gttcggccat gctgtagtcc ggcacgtttt caatgcggtc aaagccggtc atgatgatgg
    51781 cgtcgtcccc ggctacgatg tcgcaatttt caatgcggat attgctggaa gtggtgcagt
    51841 gaatgccgtc gctgttgggg atcaggggat tgttacggat ggtgaggcca tcgaccagga
    51901 cgttgtcgca gtacccaaaa cggatggccc agatcggcgt gtcttttacg gtaatgccgc
    51961 tgagccggac ccccgtgcag tgaaaaaaga cgatactcat gcctggcctg ggtttgcgtt
    52021 ttaggggacc atccgtaaaa aaccggcctt cgggcatata gttttccctt tgccggatcc
    52081 ggctccggtc aaattcagga aacagatgat ttttatcccg ctcataaaaa tcgatacccc
    52141 gggcatggat ggtccctgaa cccgtgatgg ccacctctgt ggcatcttcg caatagatca
    52201 tgccataggt ccggttgtcg accctgaaat cggtgaggtt gagactcgac tccagcacgg
    52261 aaccggggct gagatgcagt tccacatggc tttttaaaaa aaggctgccg gtgataaagg
    52321 ttccggcggg cacggtcacc ctgcccccgc ctttggcatg acaggtatcg atggttttct
    52381 gtatggcccg ggtggaccat tgacccggca cggcgccata gtccaggata ttatagtctg
    52441 ttccggctgc ataatgcgaa cccgtgagca ggaaggcaaa gaaataatgc ttcaatttca
    52501 taaaagaaag tcttgatgtg aaaataagcc aatctatgct gtgatgggag gccggaaaga
    52561 ggatgttgac gggaagggga gtaaaaacaa ctgagatccc tcttcggttt ggcccagggg
    52621 gatagtctct catggacctg atttaagcca aatcatgcaa cagggttatg gattgggttt
    52681 aaatcaccgg gaaacggtgc agggatacta atgcaataag cggaacaata aaaatatatt
    52741 taatgcatac aggaggaata tcaccagggt gtcccaggcc agcacgattt tttttcgggt
    52801 aatgggaaaa aggagtccga tgacggacac cagggccatc aggattataa aaaagacgga
    52861 caccaggtta acgtcggagg cgtccttcag caaatggcct ttggtataaa ggagatcgtc
    52921 caggaacaat atgaagatgt tgaaaaggtt gctgccaaat agattgccta cggcgagatc
    52981 cagtgatccc atccgcgcag ccgctatgga taccgcaatc tccggaaggg aggtggagat
    53041 ggccaggaac aaggtgccga caaaggaatc cgccagaccg gtatgcccgg cgatgcggtc
    53101 agcaaaatag ggcaggctca atgctgcaag gacaatgatc agggcatata cggaaaaccg
    53161 ccataacacc tgtttaggat tcaaatctcc cacagccgct gaatgctgct ccccgggctt
    53221 ttgaaaagtg cgctggtatt tataaatggt ccttattgag aaaaaataga ccagtaaaaa
    53281 tccaatgctg gtcattccga tggatggggt cagaattaaa tcatcttcca ggaaaagacc
    53341 caggcccgcc aggacaatca ggatcatacc aaaagatgcc gccaggatat ggctttggga
    53401 aaggctggta aacaaaggcc ggtcttttgg agtgaataca tccaaaagag agaggatacc
    53461 caggttgaat gcacaacttc ccaggatatc cccgacggcg agatccgccg attcaatcaa
    53521 tacagaggcg cttaccccgg acatcagctc gggcagcgag gtgactgtgg ccatcaggat
    53581 cataccaatc caggcccggc caagaccggt aaattcagcc agcttgtccc caaaatagga
    53641 aagctttttc ccggcaaaat atacaacgcc ggcgcaaacc ccaaacccca aaatatctat
    53701 acccatatca ccgatggttt ggccccttct ttatcaattt atcctttggc ttaaaatgtc
    53761 cggataaggt ctgggtaatt ctgtaaatct ttttaaaaaa attgcagacc aggtttaata
    53821 aataatcatt ctaatttata aacagcgtcc ggtaaattca aggaatagat ctattccagg
    53881 aaacttcagt cagaagtcct taaagtcacc accgatgtgc agcttaaggt ttacctaaaa
    53941 cccaccgaca ttaagccggc ctcccgttag gcattttcct gacaaggaag cagttgccgt
    54001 agtgctgttt aaaatggcgc ttttgatctg cgcggcgctg gcgcccgggt tagaggcggc
    54061 atagagcgca gccgctcccg tcacatgggg ggtagccata gaggtcccgc tgtaacttgc
    54121 atagcctgaa atgacagcgc ctttggaact cttggggatg gaggaccaga tgccggaacc
    54181 cggggctcct aagtccaccg aattggcacc gtattgtgaa aagctggaca aggcacccgt
    54241 attggtaatg gaagctacgg ctatgatgtt ggcattggga taactggcgg ggtaactggg
    54301 acttgaatcg ttattgcttc cgctgtttcc tgcggcggca acaaataaaa tcccagcctg
    54361 gttggccctt tcaatggcac tctgaagtcc ttgggaaaaa ccaccacctc cccaggaatt
    54421 attggtggca acgaggttca ggccatgcct cgtttttaag tctgtgaaat agtctatcgc
    54481 ttttatggca ttggctgtgg ttccgcccct tcttcctaaa aacttggcgc tcaacaaccg
    54541 gacgttccag cataccccgg ccactccttt attgttggcc ccggcagctc cgatcgtgcc
    54601 ggctacatga gtaccgtgat catcggtagt gccatcgaaa acagaactgt tgtttccgtc
    54661 aaagtcccag ccatagacat catctttata tccattgccg tcatcgtcaa ccccattgcc
    54721 ggcaatttcc cccggattct tcccggcgtt ggcggccagg tcttcgtggg tgtacatata
    54781 accctcatca atgatgccga tgtagacggt tgatgaaccg gtatgattat tggcccaggc
    54841 cgaggctgcc gctgaaccat attgattagc tggtgaagta gccgctccat acatgcccca
    54901 gagagacccg ttgctatagt aagtgtcgtt agaagaggca aaatgattat aaatccagtt
    54961 gggttccgca tattcgattt ccggcaaacg ctgcaggcgt tggatggctt ccccggtacc
    55021 cagcggagaa tttaccagga acaactcggc ttctccatca gccgggttaa tattggccgt
    55081 tttcatggcc ggagtggaaa tacgctcgat gatttcccca tcgatttgtt ttaaggcttc
    55141 cgaggttctc ctagcacctg attttttgat gggtttaaat tttacgagca attgattggg
    55201 aacaaactgg ccgggttcgg attgagccaa tgcttgttca gaaaccggga tatcgatttg
    55261 ttcacgctcg caggaaacca ggaaaacgaa tacccctaca acaaggataa gacgaactat
    55321 ctttttcata acattcgaat ataaattcaa ttaaataaaa taaatgaacc tgcagcagct
    55381 atcccgtatc agtaagtttc gaatatacaa aacgattgaa ataatgaacc ggataaaatt
    55441 tgataagctc cgaactaaaa tgaaagcgat tacatcacta tatttaggcc ctaattaatt
    55501 cagttaggat atgaaatttc ccctttccct cttgtttttc ctcagccttt tatcagcctg
    55561 taaaaattct tccccggaaa cggtctcatc cgggacaggg tcggatacca cctctgtaaa
    55621 agcagagccc aaagccattt cggaccctct catcaccgct atttacaccg cggatccctc
    55681 tgcccatctt tttgatggga agatttatat ctatccctcc cacgattacg aagccggggt
    55741 gccccaggac gacctgggca gccattttgc catgaaggac taccatatcc tgtcgatgga
    55801 cagcatcggg ggcgccgtga ccgaccacgg agtggcgctg gctctcgggg acattccctg
    55861 ggcgggcagg caactctggg cccctgacgc ggctttcacc aatggtaaat attatctcta
    55921 tttcccggcc aaagacaaac aggatatttt caggattggt gtggcgactt cgacatcccc
    55981 ttcggggccc ttcaaggccg aagcttcccc gatcaaaggc agttatagca ttgacccggc
    56041 cgtgttcaaa gatgaggacg gcaaatttta catgtatttc ggcggaatct ggggaggcca
    56101 actgcagcgc tggcagaccg gcaagtacga taccacaggg gttgaaccca atgacgaacg
    56161 ccccgccatg gggcccaggg tagcccgcct ccagaataat atgctgcagt ttgcagaacc
    56221 ggtgaaggag gtccaaattg tggattccgc gggcaatgcc atccagggca aggaccatga
    56281 ccgccgcttt ttcgaagcgg cctggatgca caaataccaa ggaaagtatt acctgactta
    56341 ttccaccggg gatacccatt ttatctgtta cgcgaccggg gacaaccctt acggcccgtt
    56401 tacctatcgc ggcatcgtgc ttcatcccgt gatcggctgg accaatcatc attcgatcat
    56461 cgagatcgga ggcaaatggt atctgtttta tcatgacagc agcctgtccg ggggacagac
    56521 ccatctgcgt tgcatcaagg tgacggaact gcactataat gcggacggga gcatacagcc
    56581 tgttactgcg tatttataaa gttcgacccc ttcagggtcg tagaggatga ggctatggtc
    56641 ccggggcaat gccccgggtt atgagtgttg aatcccttcg ggatcgagac cctcatcatt
    56701 gtttccataa tatagaatag ccactatttg atctttcaat ccgattggat taagttaagt
    56761 tgacccatac agtcggatat tccattccca acccctccac ttgttgcgta tgaggggatt
    56821 gggaatggaa tattcacaaa gcagagtgta agattttttt aaatattttc caaagtgact
    56881 gattcatcaa attctgacca tctcatataa tacttgttcc caggccctgg gctgcgttcc
    56941 gggttatgag cattgattct ttggggattc cctgtcgcct tttcccttac gctgctttcg
    57001 cccttcacct tattctcttt cgctgccttc acccttcgcc gttcaccctt caccttattc
    57061 tttttcgctg ccttcaccct tcgccgttca cccttcacct ttactccctg ctcctcggtc
    57121 catacccagg tcccgcttgc ccgtactcta gggcccccgg atccggcccc tttccggaat
    57181 acccatcatt gatattaggc aagaccaccc ctttgtcgat cacctccgct tcggctttga
    57241 gctggaaatc ataaccgtcc ttgggataga tcctgcccct ttgttttgga tcgggcaagg
    57301 ggacctgcat gaagatatcc gggctggtct ccataccatg cttttccatt ccggtatgtt
    57361 gtctgaattc ctccagggta ttgaaaaccc gcatgccgag ttccttatca tcagcctggt
    57421 tgagggaatc gtgcagggga gagcgccagc ggtagcgacc cggcccggca ggtttttcgc
    57481 ggtaaaaatt atagtccagg ctggaatagg tggtgtaatt ggtggcctgc agggaaggcc
    57541 tggtgtcgtc gggaccgata aaaagattgt tcctaaaatg gccgtttgaa aaattggtga
    57601 tggccgcctc cgcgataaag gtattgtgca gggtatagat cccggatgga cggaccgtgt
    57661 atttcagggc ggtgccggga atgtgataac agatgttccg tataaaatat accggccctc
    57721 cgaatacagg ctgggcgctc agggccgagt gataggaatt gaagccccgg ttctcgaata
    57781 cccggatatt atgcaccccg ccgtcggctt ctataaaatc atccgacatg ttgaagatgt
    57841 cgttgcggta aatatcgatc gatacgcagg ctttgccgtc ctcgggcagg ccatgggtat
    57901 cgatgcagac cccgtcatga aagaaggaga tcgtattgtg acagaccaca tgaccctgcc
    57961 catagacttt gatggcataa taggaggtga ggggggctgg gttttccagt ccgtaccaac
    58021 ccaccagggt atccggatcg tgccgtccgg tcaggatatt gtcggcaatg taaaaatccc
    58081 tggaatccgc gctgtaagtg accaccccga tgcccacgtc atggatcctg cagtttttta
    58141 ccgtcaggcc gctgcagccc atcacctttt tgagcccggc gtaaaaggca atgtctgtgt
    58201 tccggatggt aaggccttca aaataatgat aatcggcagc catcacatca aacaggtggt
    58261 atgctccgtc cccgtcaaaa atgacctccc cgtctcctgc ggctttgatc gtgatcggtc
    58321 tatccgctgt gcctttttgg gtaaaaacat atgccccgtg gaaatccagg gccagggcat
    58381 cggcataacg cagccgattg cccttataaa gtcccgcatg aaccaggatg atatcacccg
    58441 ggcgaaccct ggcttcgggc acgttccacc agtcgccggt attgcctatg ccataataag
    58501 cctcattcag tccggtgaag gcgggctgtt ctttgggtcc ggtatgtccg gggggataga
    58561 catggtagac ttgtccctgc ggaaagggct ggggttcagc ccgggtctgt atattgacca
    58621 ggtgctccgt ctcaccctcg attccgtccg gatccgaaag cctgaaacgg cattcatacc
    58681 aggtggccgg ttgaaggtta aaaatgctgc ctgcaaattt atccggtacg agatattccc
    58741 agcgttgttc atgtccatag atcctctccc cacctatccg gagcaagggt aaggccggct
    58801 tccagtcctg gtcccctttt ttccggtaac tgacttccac ggaggcattc cggtttttat
    58861 cccctgcgat ggaccattca aaaccggcgc aaaccagggt ggggggctcc acctcaaatt
    58921 gaatggaacg gcagcggttt tgggagggga tggtttgagg ggaaagataa ctgggctgta
    58981 ggcggaacag ggtcgagatc aagaggtgga ggaaaaggta catggtgaaa tggaatggaa
    59041 tggtatttaa agttaaagca attaaatctt accggaccgc cgtaaggaat taaataacct
    59101 gggcaaaatt aaagcggcag aattattctc attaacttgt gttgagcccc cgaatcaggt
    59161 atggaatgaa cagtcctttt tcccctcaaa aagatttctc catgaattct gcttttctta
    59221 gaaaggcaac aggaattgta ctaggtcatc tggaagacga atctttttcc ggaaacgagc
    59281 tggctgaaaa actcaacctg agccgtgaac aaacacatag aaagattaaa cagtttactg
    59341 atctttctac gggcaaattt attcgatcta tccggatatt gaaagcctgt tgttatctcc
    59401 ttgaaggcaa tctttcggtt tcggagatcg gttataaggt cggcttcggg gacccctcct
    59461 actttaataa atgtttcagg gaagagaccg gcatatcccc cggtgaaata aaaaagagcg
    59521 gcgatttgaa tcctttggct aaaacaccgc tgctttcttt ttaccaggtg cctgaaataa
    59581 aagagcagtt acggaaccat ggaatggtat ttgaatttcc ccaacaggaa agtataaacc
    59641 gcggccggcc cggaatttgg ctttccgtcg tcccgatttc tatctttttg ctgcttgcac
    59701 ttttttttat gtttggaaaa aagcgcagta caattaaaat tgaccccgga gagaacaatc
    59761 ggatcgcggt tttgccattt attaaccata ccggcgattc ctcgttagaa ggagtaggcg
    59821 atatggccag cagctggatt tccggacagc tcgccgaatt aaaaatgata caaaccgttc
    59881 cgtactttac ggtaaagcaa taccaatcct atatcgggat tttaccggga gatccactgg
    59941 gaaggcctac attccgcgat ctggttgccg cccgttattt tgtgaatgga aattattatt
    60001 taaaggatga ccgggtctat tttgacgcca gttttgttga tgccgtctcc cttcaaacca
    60061 tttatcattt gcctaccctg cacggtgcag tggacagtgt gatgaatacg attgaagacc
    60121 tccgcttaac ctttgcaggg ctcatcacca acctcgacga ggtgaaactg ggcaagcgca
    60181 aaccgcccaa ctatgaggcg taccatgcct ttcttaccgg cttgcatcaa atgaccgttg
    60241 gactggctac cccggaatcg cgattgtacc tggaaaaagc cgccgcgctg gaaccggatt
    60301 ttgtgatgcc gcggatcttt ttggtgtggt tttaccaggg aaaaaaattg gacaccttgc
    60361 tgcaggaaat tgcttcgata ccaaacatta ccacctatga gcgggatgtg tatgaaatgt
    60421 tgtaccatct gctgaatcat cagtacaaag aatcgctgcg aatagctctt caaaatctgg
    60481 ataaataccc cggggattac tttttcaact tgtttgccgg ccacaatgcc aaatcacaat
    60541 ttatgccgcg tttaaccctt aaagtgcttg accagataaa ggaccccttt cccaataatg
    60601 aatggggtat gtggcactat tataaagtat ggaattacac cgaatccctg gctatgcttg
    60661 gtcagtacca ggaagccctg gactacctgc aatccattcc tttgaaaaat tacagcctgg
    60721 ccatccctca actcttcatt caggtacagg tcaggctggg taaaacaagc cgggaacttg
    60781 aagccttgat tgaggatatt gccggggaga aaatgaaata ccttgcgggc agatttgacg
    60841 tcgacgaaca aaaagtttta gcggagtatt ataccgcagc ggcttatgaa tatcagctgg
    60901 ccaaccacag tacggaagcc cggtattttg cagataaagc ggtttcccat tacgccctca
    60961 tacccggaca gaaagcgtat aaatatgata tgctggacgc gctctacctc tcgggcgatt
    61021 atcaaaaaac gaaattacgt cttgaaaaag aattaaaaaa agatccggcc aatgacgaac
    61081 tgcttattta cctggcgcag gtggaggccg ctatgggaaa ggaaggcgat gctttgaacc
    61141 tgtttgcaaa atacgattcc ctgccccgga tgtattggcg caggcatgag tttccgtatc
    61201 ataaggacta tctcaaggca agaatttatg ctttgcttgg gtaccgggag caggcgttta
    61261 cccttttaca gaatgccctt taccaggggc aactctgcca taactgggat tttgacaggg
    61321 atgtattttt acaatccttg ttggattatg cgccattccg ggaaatggtc cgccctgttg
    61381 acgggccgat tccacaataa gacaacaaat gcaacaggag tactacgaaa ggcaacaata
    61441 ggtaactcat tgtcattcaa tgccctataa cttgatggcg tttattaatc ctctaaaccc
    61501 atttagttat gaaaaaaatc atgaatgctt ttgtcgtttt cctatttcta gttgtttcgg
    61561 gaaaaagtcc ggcgcaaagc gctgcgtcca gggaaaaagc cggtaagccg gaacctcatc
    61621 gcgaacaaca catcgctatc gaggattttc ccaaaaatat ggcgatcaac catcttaacg
    61681 gatggggggg tatgacggtt gccatcaatg aaatgcctgc cggcaccgat ctttcgcctt
    61741 tgcttgaggg tctgaaaaat aacagctgcc aggttccgca ttggggatat attataaaag
    61801 gttctttgcg ccttaaatac gatgatggca aagaagtggt attaaaagcc ggggatgtat
    61861 tttatatgcc gcccgggcat accggttccg tggtggagga tctgaaatta ttagacttca
    61921 gtcccgaaaa agagatgaaa ggactcatca gtcatattga gaaaaaaatt gcgagtccta
    61981 aacaataagg gaagatcctg gaatcttaaa gcagctggca ggttccaagt taaaaatgct
    62041 gcctacgaag ttacccggta cgggatattc ccagcgatgt tcatgcccaa agatcccccc
    62101 gatccggagc agtgaaattg cttttttcca gtcaccggcc acccttcttc cagtaactga
    62161 gttccacgga ggcattccgg tttttatccc ctgcgatgga ccattcaaaa ccggcgcaaa
    62221 ccagggtggg ggggctccac ctgaaaattg aatggaacag cagcggtttt ggggaggact
    62281 gccttccttt ggaaaatcgg tttggagggg tatcggtaat cctataaggg aaaagatgga
    62341 acagaggcat ggcgatcagg tattaatctt aaaggtaaaa gaatagacag ataccggtcg
    62401 gtaatccgtc atgaaagaaa gcgaaaccct tccacgtact gtaaggtatt tatcccatgg
    62461 atatatttgt attggttagc ggtcttcctg gcagccattt tctaaaccaa gcttaaagcc
    62521 atgtcgaagc agttttcatt catcattctg atcagcgcat tcctcatttt ctgcatgttt
    62581 aggccgttta tgcctggccg gtatgaccaa ttggccgtgc ccatttcttt tatggtccag
    62641 attttcagca taacaggctt gtcgtgggtg cccctgggtg ccctttggtt gatttcagta
    62701 cgtgacgaat ccggtttccg gaaatggggc aaccgattct tgatttacgc tttgatcctt
    62761 tcaggcctcc ttgccttact ggtttcggtc gccccgtttt ctcaaaacaa tacttccttt
    62821 gcagtcttat ttctggcctt cgtttccctg gtcctgggga tggcctatgg caaaatcaaa
    62881 aattcggcac ctttagacag gagtttccat cgggcgatac tgatatactt gatcctgatc
    62941 cctttattgg tttttacgtc cagattcttg tttatggaaa gggccgtaca attttcccgt
    63001 gatctggcca tcaggaacag cgccgggctc atccagaaaa ttgaggatta tttcaagagt
    63061 catgggcaat acccggtttc gcttcaggcc ctgcacagcg atatcgtgcc cggcgtggtc
    63121 ggcatccccg ggtattttta cgaacccaat ggccaggctt acaatcttta tttcaaaccc
    63181 ttatctgacg aactggatgc ggatgaaatc gtcatgtaca ataaacagga cgaacaccgc
    63241 ttctccgccc atgatcttga ccttttggaa tattcggggg aggcgctggc cttaaggcgg
    63301 ggcgacagaa gaaggctcag gctttcagct ccgcactgga tttcaataaa gtttgaatag
    63361 gtggataaca acgtaccggc gtcaggatat taatgtcggg tttcttggag ggctcccggc
    63421 aaatatcctc tgaatggtag gacaaagcat tcggtcgaat cggggcgacc ctggaaacat
    63481 ttgtacattt aattgctttt ttccaaagcc gttccatatt aaccaatcat gaaaaaattc
    63541 tttgtcctaa acctccttct cttaaccgcc tccctgaccc aggctaaaat cacacccact
    63601 catctccggt gcgaatacct ggaaaatccc caaacgatcg acgtccccaa gccacgcctc
    63661 tcctggatca ataccgccgg accgggtgaa cgcggacagt tccaaacagc ctgggagatc
    63721 cgggtggcca gttcaaaaga gaaattgcta gtgaaccagg ccgacctgtg gtccagtgga
    63781 aagcgaaaat caggccaatc caccctcgtt ccctatcagg gcaggccgct gacttcccgg
    63841 caggattgct ggtggcaggt gaggacctgg gacaaaaacg gaaaggtttc cgcctggagc
    63901 gaaacggcat tctggagcat gggattgctc gagccccatg aatggaaggc cctgtggata
    63961 ggagccccct ggcagggcga ggaggccatg cccaaaccgc gcttccgggc tgccggggat
    64021 acctcggcgc tgccctattc cccgccccct gcaccttatt tgcgcaaatc atttaaactc
    64081 tccggcaagg aagtagtttc cgcgcgcgcc tatgtcaccg ggctgggttt tttcgaattc
    64141 tatgtcaacg gtaaaaaagt gagcgaggac gtcctggtcc ccaacgtaac cgcctatggc
    64201 aaacgccccg gcctggaaaa aaactatatt tccataccgg atacttttga ttaccgggtg
    64261 atgtatttgt cctacgatat taaggacctg ttgcagaccg gagacaatgt cctgggggcc
    64321 atcctgggca atggttttta tgacgcgccc atgcactgga cccactctta cggatctcct
    64381 cgctttttag gccaggttca tattacctat caggacggca ccgaagagtt gatcgtaagc
    64441 gatcaaagct ggaaggcctc caaaagcccc atcgtgatgg atcttgttta cgacggcgag
    64501 cactacgacg cccggctgga tcaggcaggc tggagcagtc cgggttttga ggatgggcat
    64561 tgggaaccgg cagccctccg taaaaagccc gaaggttata tgaaagccca tatgtctccc
    64621 acggaccggg tcatggaacg cctcaaaccg gtaaaaattg aagccctcga gtccggcaag
    64681 taccgggttg actttggaca ggagatctcc ggctggctgc gcctcatcga tgtggagggg
    64741 gagcctggac gcaaaatcga tatcaagtac ctctccgaat cctatatggg caccaattct
    64801 tataccttga aaggaggagg gccggagtcc tatgcggcgc ggtttacctg gtttgtattc
    64861 agggaggtgg tgatttccaa ctggccggga ctcttaagac ccgaaaacct ggtggccgaa
    64921 gccgtttact ccgatgtaaa aaccaccggg aaattcgaga cctccagtaa cttgttcaac
    64981 gacatccata aaatctggtg gcggagccag accgacaata tgcacggggg cattgccagc
    65041 gattgcccgc accgggaacg gtcgccctat accggcgatg gccaggtggc ctgtgtgacg
    65101 gtcatgcatc attttgacgc cggcgctttt tataccaaat ggatccagga catcctgggg
    65161 gcccagaacc ggcagacagg ttacgtcccc aattgcggcc cctggcaacc cggatgcgga
    65221 ggcggggtgg cctggggcgc ggccatcaac atcatgccct gggaatttta cttgcattat
    65281 ggcgacctcg atatgctgaa gaacaactac gaaggcatga aaggatacat caaatacatg
    65341 ctcacctgga cgaatccgga aggcatcatg cactcccagg ctccggatcc ggccaaaccc
    65401 aatcaatggc tgaatctggg ggactgggcc cagccgttcg aactgcctcc cagcgacctg
    65461 gttcacacct tctatctctg gcgatgtgcc gattttacgg cccggaccgc taaagtattg
    65521 ggtgatcaaa gcgaattcct gcattatcag tccctggcag acaaaacccg ggccgccttc
    65581 cggaataaat attacgataa ggtaaagggg acttatggcc catatggcgg gaatattttt
    65641 gccttaaaaa tgggtctgcc cccggatcaa tatcaccggg tcctggccgc cctgaaggcg
    65701 gatatcctgg ccaggggagg tcacctggat accgggattt tcgggaccca gttctttttt
    65761 gaagtcctgt ctgaaaacgg cctccatgac ctggcttatg aagctatgaa caagaccacc
    65821 cagcccagtt atggctggtg gcttagccag ggcgcaacca cttcctggga acattggaat
    65881 ggaaggggtt cgcgtaatca tcctatgttt ggcggagggt tggtctggtt ttaccgggtg
    65941 ctggcaggta tgaatgcgga tgcctcaaaa cctggttacc gccaccttgt tttcaaacca
    66001 cagcccgcag gggatctcag ttttgtttcc tattcgaacc agactcctta tggcctggct
    66061 ggggtaaact ggaaaaaaca tgaaggctca ttcctcctga atgttgacgt tccggtaggc
    66121 agtacagcta cgatatatgt gcccgcatcc ggacctgaag ctgtcacgga aggtggtaaa
    66181 aaaataaaaa acaacaaaac actccgtttt gaaaggatgg atgggggtca tgccgttttt
    66241 acagtgggtt cgggaaggta ttcgtttgag gcaaagagat aggataaggt tgaggttgag
    66301 gttaaggtta aggttaaggt taaggttgag gttgagtata agtttgatgc taacaaacga
    66361 aatctgaata ctgtatactg aatactccaa tatatatgtc cgagataaaa acaaaacaaa
    66421 ccggggcgga tgtttacgaa ttcatccgga cttttgccga tacggaacaa aaacggcagg
    66481 atagcctcgc actgatccag atgatgcggg aggtcaccgg atacgaaccc aaaatgtggg
    66541 gcccgtccat aataggattt ggcaaatacc attacaaatc ggaacggagc cggcaggaag
    66601 gggattggcc cctggtcggc ttttccccga gaaaagccgc catttcactt tatgtctaca
    66661 ccgggagccc cgaacaggcg catttgctcg aaggattggg aaaattcaaa atgggtaaag
    66721 cctgcattta tattaaaaaa ctgtccgata tccatccggg ggttttgaaa aatctgatga
    66781 aggaaaccat ccattttctg caagcccggt tcgggaaagg ataatctcca gcgctaagaa
    66841 tcgaccattt tcctggagat tctgcccttt gagaatgtcc attccctttt attaaagccc
    66901 ggtaaataaa gataccctta tgccggggtt ttcatttcgc catacatagt tccgggtaag
    66961 cccagaacca gtattcttcc gcaaacctcg agtttacctg gcattttatc atccatgtgg
    67021 ctttgaagaa atgggctcgt tgccggtctt tctcacatta aattcggaga cttgaaaatg
    67081 ggaacagcag gccaaatacc catcaaacca aaaagccgat cagtccgccg atttcatcta
    67141 aaaatgaagc tcttgactta actttatgga caatgccata tagatttttt aagaacatgg
    67201 taaaaatgaa aaaattaaaa ggacctcgcc cagccttgcc tgccgggcct ggatgggcca
    67261 ggtttattgt ctatttaacc tttcttctaa aaattgagcc ggtgctggcc cagaccccga
    67321 accttgttta catcatgacc gacgacatgg gctatgggga tttgagtggt tatgggaata
    67381 aaaagtttgc cacgcccaac ctggacaaac tggccttgca aggagtgaaa tttttaaatg
    67441 cttatgccgc ggccccggtt tgcaccccca cgcgtaccgc tttgatgacg ggccggtatc
    67501 ccgccaaaac gccggtggga ttgatggagc cactccgcag tccgaccctg gacagcgcct
    67561 atggattgac tggggatatt cctacgctgg gacgcttgat caaggccaga ggctatgaaa
    67621 cggcattgat cggaaagtgg catttgggtt tcatgccgga tcagcgaccg gggaaaaatg
    67681 gattcgatta tttttttggc atcctgagtg gcggggccga ttatgtttca cacaaaggga
    67741 ccaacggcac caaggcccat gacctctatg aaaatgattc tttagtttac acccagggct
    67801 acctgacgga tttaattact caaaagtccg ttgcttttat aaaacaaaaa catttaaagc
    67861 cttttttctt aaccattacc ttcaatgccc cccattggcc ctggcagggc ccggcggatc
    67921 cggcctatcc ggattccatg gcctttacgg ccggcggctc tccatccatc tatgccgcga
    67981 tgatgaaaag cctggatgaa ggcattggta aaatcatgca ggccctggac gaggaacagt
    68041 taagccggca aaccctggtc atttttacca atgacaacgg cggggaaaaa tattccgacc
    68101 agggcggctt atcgaatagg aaatttacct tgtgggaagg cggcatcagg gttcctgcct
    68161 tcgttcgttg gcccggtaaa atcgaggcgg gaatgagtac cccccaggtc gccattacca
    68221 tggattggac agcgaccctg ctgaaagcgg ccggcgccaa ggccgataaa aatttccctt
    68281 tggacggaat agacctcatg cccgttttaa ccggcaagaa aaaggagatg caccgtaccc
    68341 tgttttggcg aacttttcaa cgcagcaaac acaaagcggt tcgtgacgga cactggaaat
    68401 acctgcagga tgaagaagga gaatatcttt ttgatcttac cgccgaccag gaagaaaaac
    68461 aggacctcag gaaagaacac ccggagaggt tcgagcggat gaaaaacaaa atgaatcgct
    68521 gggaaaaaac agtcctgccc cccattccat tgtaagcctc cggagggcca gtcattttcc
    68581 ccttatcttt gcaccttttt aaaccttatg tttcctcctt acgatgtgat cgtggtcgga
    68641 gccggccatg ccgggtgcga agccgcggtg gccgcggcca atatgggctc ccgggtactg
    68701 ctcctgacca tgaatatgca gaccatcgcc cagatgagtt gcaatccggc gatgggaggg
    68761 gttgccaaag gccagatcgt aagggaaatc gacgccctgg gcggatacag cggcatcgtg
    68821 accgatcata ccatggtcca gttccggatg ctcaaccgtt ccaagggtcc ggcgatgtgg
    68881 agcccgaggg cgcaaagcga ccgcaacctt tttgcccgga aatggaggac cctgctggaa
    68941 gcccatccca atatagattt ctggcaagac atgggcagcg ggatccttgt gaaagagggc
    69001 cgggtgaccg gtgtgcggac ggccatgggc ctggaaatcg aatccaaagc agtagtcctt
    69061 accaacggca ccttcctcaa cggcatcatc catatcgggg aaaaacaata tgggggcggc
    69121 cgcgccgggg aaaaagcggc acaagggatc accgaacaat tggtttccct gggttttgaa
    69181 agcgggcgca tgaagaccgg cactcccccc cggatcgacg ggcgaagcct gcattatgag
    69241 cggatggagt tgcaagccgg ggacgaaccg gccggtaagt tttcatttac ggacaccccc
    69301 aggctggaaa aacaattacc ctgccatatc acttacacca acggggaagt gcatgaaatc
    69361 ctgaagaccg gatttgacaa aagccccatg tttgccggcc ggattcaggg actggggcca
    69421 cggtattgtc cttccatcga agacaagatc aaccgctttg ccgataagga aaggcatcag
    69481 ctctttgttg agccggaagg atgggacacc tgtgaagtat atatcaacgg gttctcctct
    69541 tccctgcccg aagacatcca gtataaagcc ctgaaaaaga tcgagggatt tgaagaggtc
    69601 aaaatgttca ggccgggtta tgcgatcgaa tatgattatt ttcccccgac ccagctatcc
    69661 acgagcctgg agacccacct gatcaaaaac ctgttttttg ccggacagat caatggaacc
    69721 acgggttatg aagaagccgc ctgccagggc ctgatggccg ggatcaacgc ccacctggcc
    69781 attcacgaag aggagccttt tgtcctccgg cgttccgagg cctatatcgg ggttttggtt
    69841 gacgacctgg tcaacaaggg gacaaacgaa ccctaccgca tgttcacctc ccgggcagaa
    69901 taccggatgc tgctccggca agacaatgcg gacctccgcc ttacgcccct ggcattcaaa
    69961 ctgggtgtcg gaggccttga ggcaaggatg gacgcagtca aaacaaaaca aggcggggcc
    70021 gctcagatcg aagccttcgt caaatccgct tcggtcagtc cggaccagat gaatggttat
    70081 ttggaatcca ttggcagcgc aaccttatcc cagaaagtca aattgctgac cattctcctg
    70141 agacctgaaa tttccttacc ggctctcggg gaaaaaatcc ccgtcatcgg tgatttcctt
    70201 tcggcattcc ctgaagactc cgttgaactg gcagaaatcg atattaaata cgagggttat
    70261 ctccagcgtg aacgggacct ggtggataag atgaaccggc tggaagaaat gaaactgctg
    70321 gataatttta attacaaggc gattaccagc ctctccgccg aagcccggga aaaactgagc
    70381 cgtcataaac cccgcaccat cggccaagcc agccggatca gcgggatcac ccccagcgat
    70441 atcagcattt tattggtgca ttgcgggaga tagggtagag gcgagaggta agaggcgaga
    70501 ggtaagaggc gagaggtaag aggtatgaag tattactcat ctctaacctc tgacctcaaa
    70561 caaacatcct cgtcccggca tacctctctc tgaactcctt gggagtttcc cctttctttt
    70621 ccttgaacaa acggttgaaa taagattggt ttttgaatcc gcatttgaag gcgatctctg
    70681 aaatggaata actcgagtcc atcagcatgc gggaggcatt gccgaggcgg atatcgttga
    70741 tgctgtcgac aaaggtttta caggtcgttt ttttgaaaaa acggctgaaa gaaacttcgg
    70801 tcatgccgac cattttggat atttcgccca gggtgatgga tttgtggtaa ttgctgcgta
    70861 tataatcgta aaccagttcc acccggcggt tgataaaggt gggtttttca tccgcaaagg
    70921 attcattgga aagcaactgg atgccccggg tcagtgaaag gtcgtgcagg atggaaaaca
    70981 attcgaggac cgaatcaaaa ccgctgagtt tggacaattt gcggatgcgc ggttccagct
    71041 gcgcgatggt ttcccggggg aagctgatgc cgcggccggc tttttccaaa aggcttttga
    71101 taaagataag ctggttgcgc cggaggaatt tttcgtccag gaggtcttta tgaaactgga
    71161 tggtgatctc aaacatttcc cctttttcct tattgtactt ataattatgg gtcatccagc
    71221 aatggggcaa acggctaccc accagaacga gttcaagatc cccgatctct tcggaatgat
    71281 cccccaccac acggagtaca ccggccccat tgtgaataaa attcagttcg atttcatcat
    71341 gggtatggat cggaaaatca aaatcctttt tatgacgttc gaagaccagg aaacaatcat
    71401 actgggtgag gggggtgatc tctttgaaca tttcagccat aattatttgg ggttgagaat
    71461 taaaaaaaga aaagaatagt ttggagtctt ttgagcacct aatactaaaa agatactttc
    71521 atttcccgga aaaattaaca cttttatatt acatatttat ttatatataa ttgtatataa
    71581 ttgataaaca gattattaac aaaacaaagc ccttagcatt tttttaacat tccgcaatcg
    71641 attgcgatag aatcgggtcc gtaccactta tcaacagctt tttaaatggt tacgccggtt
    71701 attgaaattt cactgctatc ggaagacgat cttttcagga gagtggtatt ctttggggag
    71761 ccttatgagg atctgaattt tcctgcactt atcatttaat ggtgcagaaa cggacttctt
    71821 tcaaaatcca tggttcggac cataggtcaa cggatttgtt caaacggaat attcccgatg
    71881 cggtacatag gccagtcctt gtagtcaaaa tgccaccatt cggattcgta aacggtgaac
    71941 ccttcagcct ccatatgcct tcgcaacagg tctcgcatgg tccgctggct ttccgtccct
    72001 ccccggtaat ccggataaga cctctctgtc atttcatcgt accctccggg catctcgatt
    72061 tccaggccag tgcgcagatc aaaaaggctc aggtcaatgg cacaaccgcg attgtgcttg
    72121 gatcccttcc tgggatcggc cacaaatatt tttttctcct ttggcgtgat gtcccagaat
    72181 aatttggtca ccgaccaggg acggtaacca tcgaatatca gtaaaccgta gccttgggat
    72241 cgcagggcct ggttgaccct ggacaaggcc agggctgcat cccgctggag gaaggccctg
    72301 gcttcggtat aaacaggtct cccggccagg ttgtttggcg tggcataccg gatgtcgagc
    72361 ttgaaagttg aatcgatccg gatgagttct accaggtcgg tcgccttaaa cgctcccttt
    72421 tctgtaggag gtttcaggcc gcaggagtgg atgaataaaa agaacaataa ggctcctgca
    72481 gacacgagcc ttccggattt gggtcgcaat ggcatggggt aaaaataagg aggggtttgg
    72541 gattactacc acaacacccc atcaggcctg gctgacctgc atcattaatg caacagcagg
    72601 gggaatcaag cccaataaaa tcagccactt gccgcgcccc ccaatcccat atttgccttt
    72661 cagcatgatg aggccggtca ggttgatgag gatcagcaaa gccgcaaata cgtccgaggc
    72721 ccatttccaa tttcgtacgc tgttgcggtg aagcacattc gtttggtaga ataccggcct
    72781 gcggctgatt ccttcgtaca atcccatttt ttcatggaag taaacgtgaa gcgtagccct
    72841 gtcaaaatag atctttaatt ggtcgggggt gggaaaatca tagaccttga aggaaggcat
    72901 gcccaccata gctgccagtt tgagaatatc atcccgctga atattttccc tggacatcga
    72961 ctgatcgaaa taaaccgtat cccggttgat gatgaagtcg ggattccagt catctacatg
    73021 attcagggct atacctgaaa tgcagtaggc taggatcagg gaggcgcaga aataaccgag
    73081 atcccggtga agggcccggt tcaatttact gaatttcacc tttcggcagt agcgggttgg
    73141 atattggccg cgaattcacg gcttatattc ttaatccagg tttccacgtt tgggtcggga
    73201 agtatggcat cggcccggta agcgatccgt tgttcatgat cttggatctt ggttatgccg
    73261 tcgcctatga tcctgacctg gaacatctcg tcatgcgcaa gtaggacggg aatcacgact
    73321 gcttttgatt tttccttcaa tacccgctca atggcggcgg aggtgctgtc gggattgtaa
    73381 tgcaccagat ggccgcacca cccgtatgca taggctccta tcccggtctc ctgttttaca
    73441 aatccggcaa tatcttcaaa cagagccgac cattcgcgat tatactgggc atcgccataa
    73501 ccaatcaaga ccaaaccttc ctccgccgga ttggccgaca agacccgcgc ccttcttgta
    73561 gtgttttgtt tcaaaacccc cggaaaatca agcagggggg tgatatgggt cttcgcccgg
    73621 ggggtataac gttctatttt ctccaacttc agggtctcca tggattgggg gtctgctttc
    73681 tggccgatga tggtcggaat gtcgtcaaag gaatgagaac tcaccgtgag gaataccggc
    73741 actacgatga tatcatcgac gcccgcgcta tcgcaggcct taagctgagt ggccagggag
    73801 ggttcggtat attccatgaa ggccgttttc accacggaaa ccccgggcac gagcaaaagg
    73861 gaatctttca cttttgcttc caggtcatgc aaagccgccc tccaggtggc cgaccgagac
    73921 ccgtggttga ccaggacaat tccgatattt tttccctcac cggcattcag tgccaaagtt
    73981 tctcctcccc tcttacagga gaggagcatg gttgccagta aaaataatac ggccaattgc
    74041 cgggagcgcg aaatcctctt catagatcaa atttcctgcg aaaatagatc tatttagatt
    74101 ttgtccaaat aaaaataata aattttaaca accaaagcta tttccaaacc acccactaac
    74161 caccccccac cttcaccctc aaatacttaa tggtcttccg gttaaaagta taagcgatgt
    74221 ggaccaggcc atcctccgac tggatgattg cggggtaaga aaattccccg gcctggtctt
    74281 ccaggatcat gatggttttc caatggatgc cgtccctgga cgcagcaagg ttgaggggat
    74341 accggtttcc tccccatttt cctttgggaa tgcctgaatg attatatacc aataaatgca
    74401 tcttatcctt caaactgagg ccgtcaattc cggaattagg gttggggagt atactcgcgc
    74461 caaaggggga ccaggtcctg cccccgtcac cggaccaggt ttcaacgatg attccctgtt
    74521 ttgagcggtt cagcaattgc agctgtccgc cggggtgcat gaggagggta ggttggatta
    74581 cctcgaaacc tgaattgggg tggatggcct gaaacctgct ccaggagccc gttttcaggt
    74641 taaacgtctc catatggata ttccagcctt cgtgttccgt actggagggg cataataaaa
    74701 cggaatcgaa gagaatgggt ttgtttttta caggcccaag aaagggagag gggattcttt
    74761 ccggtttagt ccagttcttt cccccatcca ttgatttgat cagcatgccc caccattccg
    74821 aaggggaagg acccacttta taaaataaaa acaaagggcc cttcttttgc tggtaaagca
    74881 ccgggttcca gcaggggtag cgttcagcag gcgatatcat gccattggcc acttccctgg
    74941 gtttggtcca ttgacctttg tgcatccgtg aaaaccagat tcccacatcc cggttttttt
    75001 catgggtgcc cccaaaccag gcgattacgg ggccttgagg ggtggaagcc agcgtggagg
    75061 catggcattc cggagtgggt gcggcgtcca gctcatatac aaaaccggaa tccaggacct
    75121 caaccggggc ctgggcactg gaaataaaag gaaccagaaa aacaaggagg aaaacaaata
    75181 cggatttcac tttcttagtt tggctgccag gtaagataaa agaaatccgg tcaaaaaaat
    75241 gatggtagtg ccgaatacca ccgtcagatt gggatggaag ggattggaga gcccagacca
    75301 gggtcctccc ttcaaaagca aaggggaaag agacaaccag ccgattaaaa taactccggc
    75361 aatcacgcct gccagggcat gccgggactg cgtccgttcc gagataaagc ccagaagaaa
    75421 caatcccagc atgcctccgc tgaataccga tgccagggcc caccaggcat cgagtatgct
    75481 ggtcactccc tggaggctca aggcgatgct tatcccggcc aggccaacca acagggagga
    75541 taaatacaaa acctgcattt cttttttatt gcctggttca gcccggatga attttttata
    75601 aaaatctgtg aggaggatgg tcgcagaact gttgatgctg gtcgatacgg tgctcatgcc
    75661 ggctgcaaac aaggccgcca ccagcaaacc ggtcaatccg gccggcagat catggacgat
    75721 aaagtaggga aatacctggt ctgccgcgcc agggttcttg agtccgggag gcaggaaatc
    75781 aggatggacc tgcacatagg caaacagggc ggtcccgata aaacaaaaca agaaggatac
    75841 ggggaggtac aacatagagc ccatccaggt cgatttcttg gctcccgctt ccgtcccggt
    75901 gaccatatac ctctggatat agttctggtc aatgccgaaa ttctggagat taataaaaag
    75961 cccataaacc aggaccaccc aaaaggtgga ttccgaccat ccggcactga agctcccggg
    76021 atcaaactta tcccactcga tgccgatccg gactacttcc gagatacctc cgggtatcct
    76081 ccataccagc aggaaggcca cgatcagggc tccgctgatc aggatgagag cttgcagggc
    76141 atcggtccag atcacggcgc ggatccctcc catcatggaa taaccggtga cggcgaggcc
    76201 tgtgacgaga ataatccagg cctggttcca tccgaaagcg gtctgcaggg ggatggccaa
    76261 caacaaaaga atggccccgg ccctcatcca ttgggtgagc agataacaac cggcagcata
    76321 aacccttgcc cagggaccaa atcggccctc aagatattga taagcggata cgctgcctac
    76381 tttccggtag aggggtacaa aaaaacggac ggcgatccag gcagccaggg ggatggacaa
    76441 gctgaatacg aatgcattcc agttgctttg ataggtcttg ccaggcaggg cgagaaaact
    76501 gatgctgctc acataggttg cgaaaatgga aagccccgca acccaggcgg ggattttttt
    76561 ttcgccgctg gtataggcgg acacagaggt attttgcctg aaaaaactgg ccccgaacaa
    76621 gaccgttgcg ccagtgacta gaaaaaatat aaaaatatcc caggactgca agagaaatgg
    76681 atgtaagaac aagccaagat cgggagatca acccaaaata aagaaaccct tatatggtta
    76741 tcggttatta gttaaactgg ccaagtatgc ttttcaattg aacatcggtt tctttctcct
    76801 tgatttcctg gatccagctt ttgacttctt ttacacggtt ttcaagcact gcccgggcag
    76861 cggggtcttt ttcgtggacc gcccgggtga ggatcatttc tttaacctca aataaggccc
    76921 ggacaaaata aaacttgctt gttttatcca ggtttggatt tatggattgt tcaaacaaaa
    76981 agccggcttt cgaaaactga acctccgcag gaacgtcttt taaaatggcc aggtatttat
    77041 ccaggaggtt ggcagccagg ctgggatcgt ccgcaagttt ctggtcaaaa taatccaggc
    77101 tggccgggtt attttcagcg gcgaggactt ctgcaatcgc ttcctggata taaggatttt
    77161 tttcctgctg gaggtttttg acggccagac cggctttcac cgggtttatc ctggccaggg
    77221 ctttaagcgc ttccccgagg accacataag cgctgtcggc ttccagtata ttatccaggg
    77281 tggcttccag ggagttgatg cctgtacctt ccccgagtat ccggatggcg gccgcccgca
    77341 cctgggaatg cggatcgcgc agggccatcc atttgagttt gttgatatct tcaggtttca
    77401 gggaaggcgg ataatccagc gctcttaaac gaaaatacca gtagggttcc tggatcgctt
    77461 ccctccagat ttggatataa tcccgttctt cggtgagcgc attcagggcg ttcagtttca
    77521 gctgcaggtt attgcaggac ttccactgaa ttttatattc attggcggta agttcgggtt
    77581 catcccattc ggtcagcagg atccggtcgg gatccagcac gatcaggctg ggtttggaat
    77641 ccacttcgag ttcaaactgc tggtttcgat gatcgagcca tagacgtttt cgtacgattt
    77701 ccccattggc aaaatggaga tccgcgtcca aaggaaggcg gaaaaccccg ggaaattcct
    77761 tttcctgggt ctgtctgacc tggagaagga ccgttttggt gcttgcatcg aaggtatggg
    77821 tatagctcag ggtcgggtga ccgggcttca ggtaccactg gttgaaaaac caattgaggt
    77881 cctccccggt ggtttcctca aaagccagtc tcaggtcatg cacctcgacc gattgaaatg
    77941 catgccggcg gagataaaga ttgagggtgg tccagaatgc ttcctctccc acataatgcc
    78001 ggagcatgtg gaggaccagt cctcctttgt tgtagctgtg ggcgtcaaac atgttttcct
    78061 tatccgcata atgataatcg atcagatcat gggcctgtcc aaaggacacc gaggacagat
    78121 aacccatgag ttcattctcc cggtgacgat cggcctcgta tttgccgtat ttatgttctg
    78181 accagaggta ttcggcataa ttggcaaaac cttcgttgag ggtcaggtta gcccaggatt
    78241 cacaggtgac ataatcccca aaccaatggt gaaacaattc gtgggccacg atgttgtcat
    78301 tggggtcgtc aatcagttca cggtgggtct tttggataaa atccccgaag accaccgcag
    78361 tcacgttttc catggccccg ctcacatatt ttcgcaccac gacctggctg aattttttcc
    78421 agggatattt caggtccagt tttttgctga aaaactcgag catttccggg gtatggttga
    78481 atatttcctt ggcataagcg ccgtaaggtt tgtcgacata atattcaaca gggatgcctt
    78541 gccaggattc cgtcacttta gcaaaggggc cggccgcgat catatacagg taaggggcta
    78601 tgggctgatc catgacccag gtatcggtgc gggtgtggtc ggtattcctt gtgcttttta
    78661 caagtaagcc gttggaaagc gtcagcatgc tgtcgggaac ggtcagactg atttccccgg
    78721 tgcaccgttc gttgggttta tcgatggtag ggaaccagcg gctgttgtat tccgtttcgc
    78781 cctgagacca gagttgggta ggcagattgg ggtccgtccc ccggggatca ataaaatata
    78841 aaccgttgtc gtcggtaatg gcggcgctgc ccgtggtttg attccggctg ggatttcccc
    78901 ggtactccag gaagaggtcc agggtatctt tccggctgta ttttttaccg aggctgatct
    78961 tcagaatttc cccgtcatac cggtatacca ggtttttgga ctgtccgccg gcggaaatcg
    79021 tgtgaatatc aaagcttttg gcatccagct ccaggctgtc cgtaggataa aacaggggct
    79081 tgagcctcag gctggccttg ccgtgaacct gttcgttttc ccaatcaaac tttagttgaa
    79141 ggctcatatg gagaagatcc cagcgacgcg gatgactgct gcggtaagga acggtttctt
    79201 cttcatcttc tgaatacgac aggttctcat ccatttccgg gctgaccacc atggtatcca
    79261 gctggcggaa ttccatttct tcggtcagga gggaatcttc ttccgctggc cggaccacgg
    79321 atttggccga aggcctggag gaactgcagg cttgcagcaa gagcaggagg aagatataga
    79381 aggccagtgg tttcatatta taaaggggta ttaaatgggt ttgatcagga caaggtatct
    79441 tttgtggaat aatgccatgc catggtactc gaaatgaaga tcaggcccgt caaaacgatc
    79501 gggggcagaa aagtaaacag gatcataaac aggaacaagg ccccgaaaac caccatctca
    79561 atgaacagga ggcccctcca gagcgaggca tccagtcttt tacggagaaa aaataaattg
    79621 atccagcctc cgagcaggca gatcagggta aaactggtac tcagcccgtt gaacaaatca
    79681 aaggtgctcc gagaaaagcc ggcacccagg tcctgcttgt aattggtaaa tagatcgatc
    79741 agtttttctt cggtctcatt agtggccctg ggtttgccga gaaaactcat ggaatgaaaa
    79801 aaagcggtca ggaactggac cgtaatgagg gcgaataagg atttacggag cattaatgag
    79861 gcttgagggc tgggggagcg gaactcatcc gggcaaacga ggtaaagatc aaagcgtttt
    79921 gacgaaaaga tttctccagc ggaccttgat gcctcccccg tcgtggatct gcagggcgat
    79981 ctggccgctg cggctgccga ttttatcatc tttcagccgg atcattttat ggccattgag
    80041 ccaggtggtg acctggtcat ccttcaccct gatccgcata tgattccatt cccccatctt
    80101 gaggtatttg gtgtctttgc ctttggtggg tttgatgagc caaccacgtc cataggattc
    80161 gtagattcca ccggtgctgt ggcctggggg ggctacttcg gcctgccagc ctgtgacttt
    80221 ggtgccttca aaagaagagt ggaagaaaac cccgctattg ccgttggctt cctgcttgaa
    80281 ttcgagttcc aggtcaaaat ttttataggt gccggcagtg cccaggtatc catacccttt
    80341 gtccgggccg ctttcacaga tcagctcctg gttttccaca taccattttt cggtaccgta
    80401 tacgatccag ccgtccaggt ttttgaggtt aaacaattgg gccttggtct gggcgttcag
    80461 ggcggcgagg gtgcaaatag taaagattaa agtgagtctg tttttcatgt tgcattaaaa
    80521 aaattaaaaa taaggatatt attcaaaggc attccggata tccttcaggc cgacaccgag
    80581 cagttttcgc gcgatttcgt ccaggctgct ttctttgccc tggaaatgca gggtccgatg
    80641 actgtgctcg agggattccc cgggtttgag acttgcggcg ggtgaagagg attcgatttc
    80701 ataaaaaggc cccatctgcg taccgttttc caggggcccg tcgttgtacg cattgaccac
    80761 gtcccctgaa aagggagcct cctggatctt ccagagggaa ttgacataat cgccggcggt
    80821 atccggcagg ctgtattcca caatggtgag gatattcctt tcctcatcaa aagatccgca
    80881 aagaggtttc gccctgaaag cgttcacccc gatcttgctc cttttccggc catccgcctt
    80941 gaaataaaaa acaccgtccc ccgtcttcag gcggtccgca tccactttgc caaaatagtc
    81001 gtccgtgacc aatgcaccca tcgaggattc ggggccttct ttatagggaa tgaccaccgt
    81061 ggccgttgga ctgggcttga acatacccag aatccagatg gacggcatgc cggtctgttt
    81121 attccattca ttctgtcccg cattgcgtat tacgttgcgg ctttgaaaac ctacggcttt
    81181 gatgtcttcg gcctgctgga tcccatgggt ataaagaaaa gcggcagggg aaagcaggct
    81241 gacgatcctg ctgaccacga tctccatcgg ggtgttggaa tagttcttga gttcgagttt
    81301 tttgaaaaaa gccacttcat tggattcgat cgaaacgatt tcaaagggtt caatatcgaa
    81361 gccatagggc acctgccagt ggtcataaac aaagggttcg ccgggtttaa aatacaggga
    81421 aaactgtcct ccttcgggtc ctaaccaaaa ccggtcctct cctccatagg cattcatgtg
    81481 gggcagcttt tccatcgaag cgatcaggtc gtaattgagc cagccaaaac tataaccctg
    81541 gaggccgtcg gccgtgctgg tcagtacccg gccttggtat tccggcgcca gcaaaaccat
    81601 gcttttgaaa ttttcatcgt acaataaaat gggggttttt cgctgccgga tgaaccggag
    81661 gtcgtagcca aaggtattgc gttcataatc ctggaccgta ttgtcgacga tccattttcg
    81721 cagatcgttc ggattgcgga tcacaggcac ttcggttttg ggttttgatt ctttacaaga
    81781 caggcccgtg aataaggata gggcaaatag gatatatatg gtccgtttca tatcgttttg
    81841 ttttgattga taaaggcctc atgcccgttt atttgatttc ccagcggatg aaaccggagt
    81901 ttcctggtgg aggcggggca aggccccagg tgagggcgtc cttttccgaa ttgcctctgg
    81961 cgccttccac ctgtttgggg aaggcttgtt ttagtttgac gaatttcagc ccgtccgcgg
    82021 caaaccaggc gttctgggtg tcggtgaata cggcaatcac accgggcccc tgtctttgga
    82081 taagggaggg actgccgtcc agcagggccc tgacattgtt ttgtttgata tggctttcgt
    82141 ttatgttggc cgagaccacc aaaccggtcc ggggtgttcc cccccattta tccaggccgg
    82201 tataatacaa ccatacctgg ccattcaggt tgatcgggtt gggatcgtcc acccggaacg
    82261 cgtcgaaacg accggatccc tcctcgctgg cttcaaggac cggcgctgcg ctgttcaatt
    82321 tgatagacag gcggatgggg ccatcttcat tgaaaaggag tttagccagg ccaattccgg
    82381 ttttattttc cggggacccg tcctggttgg taaatccaag ccgcgcaccg acgtaataca
    82441 ggtaataaaa ccgttcatct cctaccctta ttgccgccgg tttgctaacg ccgccaccat
    82501 caaactggtc cggcgccccg acgttgatca acagaccctg atcggaccag gtatgtccct
    82561 ggtctctgga aaatgcataa tgaatggtgc ctccataccc cgaacccgca atcggcgaat
    82621 tgaccggctc ttttacataa aagacatagg aaaacccatt gtattgtatg atattgcttg
    82681 gctgggaaac gataaaccgg tcatcggctt cccatcctgc agtaccgctg atttcgacgg
    82741 ctttgccggt cagggcagac tcagtctccg cgtgcctggg gtcttgtttg caggagaaga
    82801 taataaatga aggaacaaat aaaaacagga ttgccggaat gcgcatgagg taaatttaag
    82861 agccgcaaaa ttaatgattt atgggtggtc agtcagaggt ttcaaacaac agattccggg
    82921 atattcctgc ttgccgcccc ggtaattgat tcaaattagt taattaacaa tatataagcc
    82981 gatagaaagg acctgccccg gaacctttag aaatttgcaa aaaaaattaa caaacctgct
    83041 ttcaattcag agtcaggggc ttaggaacac ttaatcattt cttcacaaac cgccttaaac
    83101 gaataccggt atttttgatc tgaccagaaa attcaaaggc aataaggtta ttcaaaaata
    83161 aactgcacga tccgcaggct gaatactatt agccggaaaa acaggaaaag atgtggcatg
    83221 aatccctggc attgcgaagt ttgatctggc ctcaccgggg gaggctgtgc ctgacctata
    83281 tccttttttc cctcgaaatg accggctctt tgctccggcc ttttttcatc ggcagggcga
    83341 tcaatgacct gattgatcac caaagccggg gcctgcttga actcgtgatc gttcatctgg
    83401 cctgggtggt catcagctat ctccggcacc gtttggatac gcgtacctac tccgccatct
    83461 ataccgccat tatgacttcc atcctttcca ggatatatcc cgggaaagaa gtcagtaaat
    83521 ggagcgcaag gtcagggctg gccaaggacc tggtggattt tctcgagtat gatgtgtatt
    83581 ttattcttga agcggcttat aatattttcg gttccctgat ccttttgagc atctaccggt
    83641 ccccggtggt ctggttatgc cttgccgccc tggtacccgt tgtcctgatc ggtttctggt
    83701 atggccgaaa aatgcggtcg cttcaaaaag cgaaacacga tgaactggaa agccaggtag
    83761 atattctgac caccggcaat atttcccgga tcaaagatca ttaccaccgg ctccagagat
    83821 ggcagatccg gatctcggac caggaagcgc tcaatttcgg gctgatggaa atcattgtga
    83881 tctcggtaat caccctggcc ttactggttt ccacgggtcc ttcggccgat aaaatccatg
    83941 ccggggacct ggtgggcatt tattattata ttctcaagtt cctcaacgga ctggatacca
    84001 taccctatac cctccagcgc tggagcatgt tgggggatat cctccgcaga ttgcaacccg
    84061 ggccggtcag cgggatcaaa acgatagagc aggaggaggt ggtaatcaaa gaagaaaaac
    84121 tggttgcctg aaattacgcg tccttaggtc cggatctttc catggacctg gagtaagggt
    84181 tcgggagggc ataaacattt atcgttttcc ggaaattaga aagttcctcc ctggttttta
    84241 cggggacgac cctggcccgg acatcaaagt cctcgaagaa aaacaggtca taatgatgat
    84301 cgtgaatgag gtcaaacaac tcgctctttt cctgcgggct gcttttctta aaaacttccg
    84361 cgacgagcac gggccggaag gcttcaatca aatcggaaat ggaccggatg atttctttgt
    84421 cataaccttc cgtatcgatt ttgatgaagg atagtttgtt caactggccg ggatattgtt
    84481 gttccaggaa ctttttgagg ttcactccct tgattttacc cggctgaatg tatttcccgt
    84541 gtatgctttc tttggtcttt gaaatgcccc cgttggcgaa ggaagcttcg gaagaaataa
    84601 aataaaattc ctcctcctct accgtgatgg caaaaggatg ggcgtggata ttggttttat
    84661 ccctgttcaa caaggcattt tgccggagaa ttttaaacac aaacggattg ggatcgaatc
    84721 ccagggtggt accctccggg cctgccgcca gggccatggg tacggtggta tccccgatat
    84781 tggcgccgat atcaatggca aggccccggg tcggataaat tgtttgaaaa aatcgaccat
    84841 tccctgtttg atttcaaacg gcctgaccag ggggttatcc cagtttgtaa attccacccg
    84901 cccttcctga ggaaggctaa agacatctgt ccgggtagca tattcgcggg tgatccgcct
    84961 cgcaagtttc ttgcggaagg ttttttttaa atattcgagt attgccatag ttaagggaag
    85021 cgctggaaaa gccggccaaa attaagcgca cgccggacat tcctgtaatt tttgtaatga
    85081 atcctccgtt tcccttctca gtcttgggaa ttgcgggtca atacgctgtc cgcgacccag
    85141 ctcatattct gctgcatcat acagttaaag tggcctttgg ggcaagcctt gcggccaaat
    85201 cttgcacagg ggcggcagga cagtccttta atctcaaaac ttaggttctg atcttcccgg
    85261 ccattgccgt aaaagggcca gaagccaaat tccgcgaggg tgctgcccca gatgcttcgg
    85321 atgggttttt gcaatgcggc ggcgatatgc atcattcccg tatcaggggt gatcacggcc
    85381 agggcctgct gaaggatgga ggcggattgt tgaatggtga aggtaccaca ggcattatac
    85441 acccccccgg tcacgagccg gtctgccgcg gcctgttcat cctttccgcc aaccaggact
    85501 atggggtatt ttccattcac cagagaaatc aattcctgcc atttgacgac cggtggcctt
    85561 tttgtgaaat gggcggcccc aatgaccagg gccaggtagg gtcctttaat cccaaaatgg
    85621 ccgtgcggat ccacccggtc ggcatcgggg ataaaaaaat caagcccttt gccatcatcg
    85681 aaaaccttgt attttctgag gctgttcaaa tagcggatgg ctatatggtc tttagagaaa
    85741 cgattgatct tgaaatatac ggacaaccat tttgagaggt tacctttttg gtaggaagtg
    85801 gaaggtaccc gccagagggc aagctttacg cggagggatc tcaggttttt atggagatcg
    85861 atgatcaggt caaatttttc tgctttcaac agcggcagga tcccggacca accttgttcc
    85921 agggtccaaa cccggtcgat ccagggattg gcgttgagca atccggcaaa gggcggtttg
    85981 gttaaataat ggatttcagg gccaatctgg gtcttgaggc agcggaccag taaagaagta
    86041 agcaccacat cccccaatgc gctgaaccgg atgatcaaaa cctttttcac cggcttccct
    86101 gctttatgat tgttgtggat ttcagagcct gataaacttg agacttccgg tatggccttc
    86161 aatagtcagc gttaatgaat agatgcccgc ggacagggaa tggatgggaa tgtccagggt
    86221 ttgatcgccg ccggcgaaat ggccggtgga cgaccatttc agccgcccca tgacatcata
    86281 tattgcaaac cggtttcctt cgctgggttt gggctgggtc cagccgaccc ggattttatc
    86341 cctgctcacc gggttgggag ataagcggac cgcataggcc tggagggatt gatctttaaa
    86401 cggagtactc aggtcccggg cgccgttggc aaaccaattg ccctggcggg catttgagat
    86461 gagggccgtg gcggttccgg tgccttcaat tttggaattg tttacggaga tctgtccgat
    86521 cacctccgta ccggactggg tgctcgcatt gaagatgacc ttgtcatcga gggtggaata
    86581 agaaggccag gccagttcag aatttataaa tatggtatcc aacctgcccg tttccaggtt
    86641 tgctcccaac aacagatagg taaacggatc cgtatcttcc acgcgcatgt caaaggcaat
    86701 aatgtacggg ctgtttttgg aaaaggtggg attgccgacg ctggtttgtt ccgggagctg
    86761 gctgaaaagt ttgacaatcc ttccgtcccc ccagtcctgg gtggtttgat cgtaaacccg
    86821 caggaaggag atatcccaat aattgatatc cgcccagtcc gtgttcggga tgctgttgta
    86881 cgcgtcgtac atcagccact cgccgctgaa atcgaattcg atggcgtcgg cataaaggac
    86941 ttcccccgtg gtgattcctg aggtgtaagt gggattatac agatcaaagg tccgccattc
    87001 ccccccggaa cccaggccga agtcatagat atgtatcctg ttttcttcct ggtcctgcag
    87061 agccgcgatc cggtttccat ccctcgaaat ggccacattt ctccaaagcg gctggtcctg
    87121 gagtatgccc tcatcggtga tctggctgat attccagtca acgatgacat aatgaatttt
    87181 tttatcctgt cctacaaaga caaatgcgga accgtcatcg gtgacgctgg tgcggctctc
    87241 cggcctgggg tctttggacg ataagggatt ggctaccagg tttccggcag gatccgcaag
    87301 gtatagggct gtggaattta cgtcggcata tacgataaag tcctctccgg gattggtctc
    87361 ggcattttgt tcggtagggg ttggtgcacc atcaaggata cccaccaggt cataggcggc
    87421 cttcgccgca ttgacttctg caccggaagc cccgtatagg tctgtcgcag cctggatgac
    87481 cgctcgcctg caatctacaa accgggaact ccgggtcaga tacagggtca gggcccggta
    87541 atagatcttt tccgctttat ccaaatcatt attgatccgg atggtaaatt gataaaaagc
    87601 ccagttggga attccgctgt tgatgtgcac gccgccgttg tcggcacttt ccgtaaagcg
    87661 ctctccatag accctgggtt ggtatccggg gtccccaaat tgaagtcccc cgttgtgggg
    87721 atcgctcagg ctcctcaggg ccccgctggg gaaggcggat aatttcacca cagattcgcc
    87781 aatgagccag tcgtcgcggt caatcaaccg gccgaaaata tctgcgaagg attcattgag
    87841 cgcaccgctt tcgccttcgt agtccaggtt ggccgtcgac tggatcaccc catgagacat
    87901 ttcgtgaccg gcgacatcaa ggcccttggc caggttggaa aaggcctggt cgccgctgcc
    87961 gtaaaacata gcctccccgt tccaaaaagc attgtccatc tggctgccgt cctcttcggt
    88021 aatattaatc atggatacaa tgttcccgcc ctgaccgtta atggacaacc ggttgttctt
    88081 gctcaggaaa tattgataag ccaggcccgc attattgtgc gcggaggcgg cggtagggct
    88141 gatccagccg gtattggtgg atccgttgat caggttggcg ctaaaattcg ggttggaagg
    88201 gctgttattc ttgccgtcaa aagtccagat gacacccgag ggctcctccg tatccgtccc
    88261 gatgctttta aacatggacc tggaggcatc cagcaggtaa tatctgcccc cttcttcgta
    88321 agtgttcagg gaaagaaggg agccgttgag atccattgcc ggcgtgctgg tttttcctgc
    88381 aaattcaaag gagggatttc cggtatttgt atccgttacc ggatttagtc cggcttctgg
    88441 atggcctttg caatgatcat gaggaagcag ggagcaggtg tttttgtatt ttttaaagat
    88501 ctgtccctgt tccgcgtcca cgatatattt ccagatctcc agtccgttgg cgacatagga
    88561 gatttcatag gtcagatgcg gacggtcggc ggaatcgtga aaaatggtaa gcacggctgt
    88621 gctcaggggc cggttgagga tagctaaaag ttggtccgaa accgcatgta ggcggccttc
    88681 tgcttccagg tcctttttta cgatgtccag ggcagcggct gcttggatcc ggggatgcgt
    88741 gttgagggag aggggggtgg gataaaccgt tccattgacc tggctgagtt gatctttgaa
    88801 cccgtgaatc accagctctc cgccgtaaac cgggatgttt tggtaaacct gacggacccg
    88861 gatatgttgc atgttcactt catcggtttc gcgggagacg atctgaaatt cctggtccac
    88921 ggttttaatt ttcatggctt cctttaaggg ttccaggaag gccgagaccg aggattgcgc
    88981 gttgcgttgg ttaaaaccgg tttttttact gctgttagcc cagaaggaat gatggtcttt
    89041 atggatgtaa tctatggcag tgggcctggc agcggttttt ccgggagtaa attttaatgg
    89101 acggaattcg ttccatgcgg gcggagcctg tttgtggcgg ctgacaggaa gcggcaattt
    89161 tttcatgggc tgcaggacct ggttcttttc tgtcctggag gtgaagggaa tggatttgaa
    89221 aggaacctgt cccgtgagca ttccggagca aaaaatgaaa acaataaaaa atctcataat
//
LOCUS       DAOQXT010000003        78115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_318_length_78115_cov_7.425993, whole genome shotgun sequence.
ACCESSION   DAOQXT010000003 DAOQXT010000000
VERSION     DAOQXT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 78115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78115
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_318_length_78115_cov_7.425993"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..2606)
                     /locus_tag="PKM27_00575"
     CDS             complement(111..2606)
                     /locus_tag="PKM27_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR05772.1"
                     /translation="MIKQTLLFLTLISCLATRTNAQFPMGGGAAGPTVTGKITGNIID
                     STSREVVEFATVALRRVGSTKDINGTLTDEKGGFKLENVSNGKYEIVISFLGYETKTI
                     SNIELTPKKPDAQVGTILLAAEGVALQTVTVEDQASLIENRVDKMVYNAEKDATSAGG
                     SAVDVLQKVPMLSVDLDGNVSLRGSTNLRILINGKPSGMFSSNVADALKMIPADQIKS
                     VEVITNPSAKYDGEGSGGIINIITKKKNAEGTSGSINSALGTRQNNLFLNLGVRKGRF
                     GLNGGGGAFWGWPGEGTNSLDRTNFVGNKTVKTSELGVNTTSRIGYHGNIGADYDFNA
                     FNSISSTFRANGFGMDRNSVTNVSVDDQILNQKLLYGRINESDILRGGFDWSTDFRRT
                     FKKKDQEFGLAFQWSNNQSLTDNIFITENNPEYNFNQKSDNDGINNEYTLQADYTHPF
                     TSKIKMEVGAKSVLRDINSDLNYFNFDPDQQVYLPDAGRTSVFKYSQDVMAGYASMTA
                     GLSKTISMIAGVRWENTKIHGRFTTGAGDPFSNDYNNFLPNISISKQMKNFRTIKLGY
                     SKRIQRPSLFFINPFQDAQDTRNITVGNPLLRPELTDQLELALTTYLKGSMVNLSFYA
                     KHTDDIIENLTEIRNDGTSEATFKNAGDNYSYGVNAFSSVNLSKKWTLRGNVNIGSYN
                     TKSDLTQENFSAGIQYNAFIMSSFDFKTKGLKADLFGIFNSPRRSLQGTTPSFSMYSI
                     GLKKEILKKKGSLGFNTVTPFNRYRAFKSSIQGDNFIQESNFKIPFASFGINFSYQWG
                     KMNFNAPQKKRGIKNDDLKQGDGGGDMGGGNRN"
     gene            complement(2785..4332)
                     /locus_tag="PKM27_00580"
     CDS             complement(2785..4332)
                     /locus_tag="PKM27_00580"
                     /inference="COORDINATES: protein motif:HMM:NF016553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05773.1"
                     /translation="MLDHFASLYRTLLLDKFPENRILALEEFLRKNEEMENKLALDLL
                     HAYKIKPLVRRSQLLEWLLEYTKLPEWLIKESHDVTGDWSETFSLIIGNPQIRSSGYS
                     LRRFLDEIDQSKNKLENLRTLTLSFWDKLSGPELFFFHRLVLGTYKPLLEQGFLSRFL
                     SKQYRVEQALVSFRLNRQSLGPESFRELIDPVWQKTEARARPIPFREISAQDPESGSG
                     VQEANWIAEPFVSGIRVQVHLINAEVHFWAYGEEYMPSPIEDPLSFFGFLPEGTVLEG
                     ILTGPPVVKSQVKGEKRKGESGWKFLAFDLLQLEYQELKSHSLSSRKQKLDELFKDPV
                     RFGIRVITYKVLQNLEDLPLLESPTGWVLKSLDEPYHPEQIPWIRIKPPGRQVNAVLM
                     YAEAIPARLSVYQCTFGIYNEDQTLVPVVRLIPEGLPDPLVTRLHYWIQNNTLQRFGP
                     VRVVRPQQIFQIGFDDVIENQRLKARLTLKNPKILAWLPDREKSLEIGSLDQLKNPTD
                     PSVFPME"
     gene            complement(4337..5368)
                     /locus_tag="PKM27_00585"
     CDS             complement(4337..5368)
                     /locus_tag="PKM27_00585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HNR05774.1"
                     /translation="MHREPLLSFTPKGIYCSRADVYIDPHRPVHRALITHGHSDHAIA
                     GHQYYLCTQLTAAILRYRLGSFYQIQTHDFGIPLDINGVRFSFHPAGHIPGSAQVRVA
                     YRDEIWVVTGDYKTEADALCENYELVPCHHLVTESTFALPVYQWQPQPVILGEILDWY
                     QKNQEQGKSTLLLAYALGKSQRLIHLIPPEIPVYTQGSVEQTTDILRKAGLKLRKTIP
                     LSSRLTKKEIQKGITLTPASEINNPWVRSLISPVTATVSGWMTLNTIRKNQAANHGFA
                     LSDHVDWTSLNQVVRACGARKIWVTHGYTEAYSKWLKTLGYEAEAVREGYRFEANRSA
                     ENQERAGED"
     gene            5432..6046
                     /locus_tag="PKM27_00590"
     CDS             5432..6046
                     /locus_tag="PKM27_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNR05775.1"
                     /translation="MKSLPWIIMGVLTLAVANLYFKVYDKSGSGQIMTGSPSEGNPLR
                     IVYVNADSVLENFTQFRKEKDALDKKQLDIDTRLGVKGRALENEVATIQQRIQSGLLT
                     PKEIQDQEQRLGARQQALLAERDKLAKEIVDEGTAINDRLQKVLIEALEGLKKEKGYH
                     YIFSYVRGGQILLGNPADDITGDVLAILNKTGTGAAMDTLGGNQ"
     gene            complement(6119..8560)
                     /locus_tag="PKM27_00595"
     CDS             complement(6119..8560)
                     /locus_tag="PKM27_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR05776.1"
                     /translation="MGLRYCFSLLGILMFKTLLAQSSGILEGRVLDLNGNKLAFVSVY
                     AEGTDKDTVTNADGDYRLVLAPAVYTIRFASVGFEPVSQVIEVGTATTLDIRLREGLY
                     ELPEIIIGRQTMTGGSLYVKDIPGSAHYISLPELQKFNYTDIHRVLRNIPGVNLQEED
                     GFGLRPNIGMRGSGVERSSKITLMEDGVLAAPAPYAAPAAYYFPTAGRVFGVEVRKGS
                     SQIKYGPFTVGGAINFLSTPIPHEIAAGVNLSVGNFGRRVVHATAGQSFTHGGFVVET
                     FQNKADGFKKLDNGGPTGFLNQDYLAKVRFNTAPGAKIYQAVLIKIGQSEGKADETYL
                     GLTEGDFEAAPNRRYHGSQMDQIRTKQHQYSIKYSMIPARFLNVSVTAYKSDFKRNWY
                     KLDQVKYGTGSKTAIAAILDDPDTYAQQYNLLKGLTSPNADALFVRNNNREYVSKGIQ
                     GILGINISDGALEQDIEFGFRFHHDEEDRFQWDDAYAMDNGVMKLHQKGNPGSESNRI
                     SKAAATASYVQYTLKSGNLKVLPGMRYEKITLSREDFGKEDPSRTGSSLKEAVNKVGV
                     WIPGIGLEYEFNSFSMVFGGVHRGFSPPGVADGSLPEKAINYEIGSRFTRRPFNGQAV
                     VFLNDYKNLLGSDLAASGGGGVGDLFNAGKALIYGVEAEAGLILTGSGKNWSMPFNLA
                     YTYTRGEFNSAFEAANEDWGKVEKGDEMPYISAHQLVFNAGLERRRYSIHLSAKYNSP
                     MRTVPGSGRWIETERIDHNLVFDLAADHTLNRHLNLFGSINNLFNEVYAVARRPAGLR
                     PGMPRGVMVGIRAKL"
     gene            complement(8693..9400)
                     /locus_tag="PKM27_00600"
     CDS             complement(8693..9400)
                     /locus_tag="PKM27_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HNR05777.1"
                     /translation="MSAFWAECIGTAMLILLGNGVVANVNLKRTYANGSGWIVITFGW
                     AIAVFVGVMVAAPHSGAHLNPAVSIALAVMGKISWLLAVNYMIGQFLGAMIGATLVYL
                     VYRDHYDSTEDRGSKMATFCTSPAIPNTLNNLLMEAVGTFVLVFAVLHIVSPEYKLGA
                     LDALPVALIVLGIGLSLGGATGYAINPARDLGPRIMYTLLPIRDKQGSNWTYSWVPVL
                     GPILGAVAAAVLYGMLH"
     gene            complement(9467..10855)
                     /locus_tag="PKM27_00605"
     CDS             complement(9467..10855)
                     /locus_tag="PKM27_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR05778.1"
                     /translation="MKKWPLLVLTMFMGKPAIHAQEVLSLTAPSQPIVFAGVNVIRPG
                     SETILTDQIVIVENGKIKELGRAGRVKIPTSSLAIDGAGKYLLPGLAEMHAHIPAPEA
                     NGDLSMVKQTLFLYLCRGVTTLRGMLGQPFHLNLKEKLAKQGITNMPVVYTSSPSFNG
                     NSVPDVSTAEKLVKQYQSEGYDFLKIHPGIKVEVYDALTRTAKQVGIPFAGHVPVEVG
                     VRHAIQSGQLTIDHLDGYIEAMAPPLADLNQNGFFGFNLTDQADVNKIKGLADLTKQK
                     GTWLVPTQTLFTRWFSPEDPAAMMRQEEMAYMPSRTRFAWHQSKTNLIKSAGYTAEKY
                     KAYLDIRKKLLLDLHQSGVRLLLGSDAPQVMNVPGFSIHHEMESWAEAGIPAWKILQA
                     GTSEVSRFFGTEKESGDIAVGQAANLILLEQNPVERIENIKTISGVMNQGIWMPKTVI
                     EEELRKIAQANQ"
     gene            complement(10860..11105)
                     /locus_tag="PKM27_00610"
     CDS             complement(10860..11105)
                     /locus_tag="PKM27_00610"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNR05779.1"
                     /translation="MWKEEKDQLRASFQFADFAQAFSFMTEVAFACEKLDHHPDWHNV
                     WNRVDIILTTHSAGHKVTEKDWALAREIEKIYERRKN"
     gene            11208..12677
                     /locus_tag="PKM27_00615"
     CDS             11208..12677
                     /locus_tag="PKM27_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNR05780.1"
                     /translation="MSVIKKLAGETALYGLSSILARLVNFILVPLHTAVFKERGDYGV
                     VGQIYGWIALGVVLYTLRLETAYFRYASQDPRSASYRTIKTSIAGIALMGSSLIFLFS
                     GPIAVFLDIGGYGFLIRLAAGIIALDALCELPFARLRLEKRPRRFVAIKTINILLNVA
                     LNFFFLWWLPGRGAGSFDESQLVSYVFLANLIASTVTLMLLSKEFLTEGFALDRKEIK
                     KVFNYSLPLLIVGLAGVIDDAMTRQFLGWLAPGTLAEKQIQMGEYNACVKFAVFISLF
                     VQAFRYAAEPFFFKHMNHENARQDYSRITTWFTRFLMLGFVVVLLYLDFYKNLIVRTP
                     EYLRALGIVPIVLLANVCLGIYYNVSNWYRLTDRTQYGMYSALVGVGLTLGFNLLLVP
                     RYGYMGTAWSMLICYFGMTLITYIWGQKYYPVPYELGKIAGALFSGIAIYLVSLFLKN
                     QFNWSPWSQFLVNSLLVGVYIFIFFGNLIREQVKRVRVS"
     gene            complement(12698..14494)
                     /locus_tag="PKM27_00620"
     CDS             complement(12698..14494)
                     /locus_tag="PKM27_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05781.1"
                     /translation="MTKNDSKTPFAGNYWTSNPEAHGPSYRICLGLFSIFAWTFPLIS
                     QGNVIPLSSKQEYLIERMSIKYGVPSGFHRAVRPYFRKDLVDLVFYWDSLKVLDPADE
                     ADIRYLFDENNEWLMDGGHTVRSKSGTGLDSMGYRYSSRPVLKHFYRTPAHFLEVNKK
                     EFYLRLNPVLNVQAGRETGAGDWVLANQRGADLRVGLDQKIYAQSTILENQLSLPAFF
                     RNKVEKNKALPGAGFYKYFSDKLYKPQGYDFFISNAHLGFRLSPHFHIQLGHGSHFLG
                     DGLRSLFLSEYAQEYFFLKLQARVWKLHYQSLFAELSASTPTASTAGNVLLPKKYTAT
                     HTLQAQLSSRWSLGLFETVIFNRNNRFEFQYLNPVIIYRSVEGSIGSPDNVMLGINSR
                     YDFGRKISVYGQVLIDEFVRKEVFSSGKGWWGNKFGGQLGIKYIDAFGIPRLDLQAEY
                     NSVRPYTYTHFDSTANYTHQRTALAHPLGANFKEYLLNLRYQPAPRWQIHAYLMAYLW
                     GDDPMPSVNYGGDLLKPYTTRIMDYDNKTTQGRLRNIRHLGFNLSYELFHNYFIDLNG
                     YYRQDLEDASDISRFIGAGVRVNFWRKDLNLL"
     gene            complement(14436..15503)
                     /gene="lpxK"
                     /locus_tag="PKM27_00625"
     CDS             complement(14436..15503)
                     /gene="lpxK"
                     /locus_tag="PKM27_00625"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNR05782.1"
                     /translation="MIRNTLVKLLLAPFSLLYGLAILIRNLLYRQEILRPARFDIPVI
                     SVGNLSIGGTGKSPHIEYLVRWLKEYLPVAILSRGYKRESTGFRIGQQAPSVKSIGDE
                     PFQFLMKFPEVPVAVAENRELGIPLLLKSYPGTRLILLDDAFQHLSVKPFINILLTSY
                     DLPFTRDYILPSGRLREWRSSYRRADILVVTKCPSGLTPEEANELKEELKPFPHQTLY
                     FSEYHYGTPYFIFNPGLRLRWNPDHYALLVSGIANTDYLQAFVRSQIKYLHLFPFADH
                     HWFSNYDLGQIKRQFDSLPGPGKFILTTEKDATRLSIHRDYILENKIPIFVLPVEVRF
                     LFNDEERFKNTIRGKLLDFKS"
     gene            complement(15500..16786)
                     /locus_tag="PKM27_00630"
     CDS             complement(15500..16786)
                     /locus_tag="PKM27_00630"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNR05783.1"
                     /translation="MKHSEELLYYIWKHRCFDHSLLKTASGEPLQVLQPGIRNHHAGP
                     DFLQSIIRIGQTTWAGNVEMHFNASEWTSHRHETDEAYNTVILHVVWSADAEVFNEAG
                     LKIPCLELQSRVDRQMLKQYEDLMQNQKWVPCEGLFHTVNDFIKESWSHRLMAERLES
                     KAVAVRQALDVQGQNWEEVCFQKLARALGTQVNGDAMELLASCTPLHLIGKHKDQLLH
                     VEALLFGQSGLLEGPFEEDYPLRLQAEYSFFKNKYHLRPMNASHWKFLRLRPANFPTI
                     RIAQLAKIIYTTDHLFSKLMAAQTIKEIINSLDVTLGGYWTDHYSFKSESRSGRFKKL
                     GLSTIQYILINAVAPLLFCYGESQDDLQRKEKSMAYLQDLPAESNALISGWIKLGWNP
                     KHALHSQAQIQLKTQYCDEKKCLQCSIGHQILKFAP"
     gene            complement(16857..17606)
                     /locus_tag="PKM27_00635"
     CDS             complement(16857..17606)
                     /locus_tag="PKM27_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR05784.1"
                     /translation="MQKILALIPVYNEEASIGPLLKNFDLVFASSGGEVEVLIVNDGS
                     TDRTMEVVRNYQGPLPLRCLDHAVNEGLGKVIREGLTYASDHLQDEDLVVTLDGDNSH
                     HPDLIPAMVQAIRQGSDIVIASRFRPGARIHGLPWSRRQFSVLARLLFSLFAHIPGVR
                     DYTCGYRAYRVSLLKKGFAHYGDRFIQETGFSCMVEILLKLARFKPVCLELPLVLRYD
                     LKAGASKMKVRKTVYKTLALLKDYWFSRRFR"
     gene            complement(17608..18789)
                     /gene="wecB"
                     /locus_tag="PKM27_00640"
     CDS             complement(17608..18789)
                     /gene="wecB"
                     /locus_tag="PKM27_00640"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNR05785.1"
                     /translation="MALPALKVAVRAYLAATMPSGKLKRILFIIGTRPEMIKFAPLIL
                     KMRREENFEPVVCFTGQHKGLLDDTAAFFGIRPDHHLNLMRPDQTPVSFLSRSLKRLD
                     KIIEKENPDLVFVQGDTSTVLSGSLAAFYHKVRIAHLEAGLRSHDKFNPYPEEMNRLL
                     CSRLADFHFAPTRTAAENLVAEGLTRDVFVTGNTVIDSLFLCLEILDSQDETEYVDYF
                     NQIDWSKKIILVTCHRRENFGEPLDRILAAIEKFANRFPEVQIVFPVHPNPNIRQMVY
                     DRLEPIPNILLTASLPYPYLVWILNKSYFVVTDSGGIQEEAPYLDKPVLVLRTLTERV
                     ESIQAGTAVLVGNQQNTILENMVRMYEDETFYRSFQQKAHPYGTGDSADQILRILKER
                     L"
     gene            complement(18922..19353)
                     /locus_tag="PKM27_00645"
     CDS             complement(18922..19353)
                     /locus_tag="PKM27_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05786.1"
                     /translation="MKTFLFGVFSLLLISVLFSRCGVMFGGSKYHASILAKDHPNAQI
                     YVDGNKIGQGSATGLFSRNRLLSVELRQEGCEPKTQIFDKTLRTGNFILSIISWGLIG
                     VAVDLGTGSAYKPDHKTNSAIQRLTVKDYSFTVDYSGCTVK"
     gene            complement(19702..20223)
                     /locus_tag="PKM27_00650"
     CDS             complement(19702..20223)
                     /locus_tag="PKM27_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNR05787.1"
                     /translation="MKSFYCRILVVLVAACISCRTSSPSGLVLNPEFDKEISSILDFS
                     IPVISVEEALDAEGVLFLDARERPEYEVSHIPGAIHIGYDHWDKTVLDSISEDREVIV
                     YCSVGYRSEKIGIKLKSLGFSRVHNLYGSIFEWVNVGLPLEDSLGGQTQRVHTYNKNW
                     SKWVTSTTVEKIW"
     gene            complement(20252..22573)
                     /locus_tag="PKM27_00655"
     CDS             complement(20252..22573)
                     /locus_tag="PKM27_00655"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNR05788.1"
                     /translation="MKFITLFFFSLCFLATHAQTGINKDPFRQLDQLLPTPNDYRNAG
                     GAPGHRYWQQQADYSIQLELDDEKQTVSGQEVIVYKNNSPDVLPFLWLQLDQNQMAEN
                     SQTYELNSHKMDPRMTKAQIDNLDHTFDGGFKLEYVKDHQTGADLKKTVNYTMMRVEL
                     PKPLKPGETYSFRIKWWYNINDRMKLGGRSGYEYFPEDKNYLYTIAQFHPRMCVYNDL
                     EGWQNKQFLGKGEFTVGFGNFEVAITVPSDHLVAATGELQNASEVLTKEQLALLAKAR
                     NERVQPVIISSQKVAEEREMKRATTKKTWKFKAVNVRDFAFATSRKFIWDAMGVPQSD
                     GRIVMAQSMYPKEGNPLWEKYSTKAVAHTLKWYSHYTFDYPYPTAWSINADRIGMEYP
                     MICFNFGRCEKDGTYSRQVKYGMISVIIHEVGHNYFPMIVNSDERQWAWMDEGLNSFL
                     QYLSEAQWEPNYPSRRGPANQIIDYMKMDQNLLEPIMTNSESIQQLGNNAYHKVAVGL
                     NILRETVMGRELFDLAFKEYARRWAFKQPYPADFFRTMEEASGVDLDWFWNGWFYSTD
                     YCDLAMTDVKWLKVDPKNPDKLAEMKREQETHALKYISRVRNESEIKQYYSDQDTTLR
                     DFYSSYDPGEPDALARQDYERFYRNLSEDEKKLLGSGDHFYEVTFERKGGLVMPVIVQ
                     LQYEDGTKEDFRYPAEVWKSLPEPKITKVFRTSKEVKRIILDPYLETADVNGEDNFYP
                     RQQQEPSRFELFKQQQRPAGENEMQRAKRAAKT"
     gene            22767..25040
                     /locus_tag="PKM27_00660"
     CDS             22767..25040
                     /locus_tag="PKM27_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR05789.1"
                     /translation="MKRILYTWIILALASELSAQMEARTPTPEDYRRAASFQWQNLVN
                     KKIFNVNIQPVWCGDSTGMGFVTQDRERKSFNKVVWGVTKIQAWFDQEKLAKALSDST
                     KQKVTASDLALQNINYKNSSHLEFHFQGKRYELNLLTHSLKILNPPASNPLESKSPDG
                     KWIAFTKDYNLYIRSTADGSERQLSRDGKRLYEFGTFYGWSDIIEGENGERPARLFAS
                     WSPDSKWIQTYLCDMRIAKKMYLLDWSVDTLFKPRLLSYYRGSPGDTDMVRMIPVFYN
                     IETGEEQIKNHLQSTHTNPIGYEWSKESGIVYQENAIRGYQAVELHRCDLLRKTEELL
                     YRETSKTNIDQYSSMILEDLGKVLITSEREGWKQLYLLDLKTKDLESLTRGNFYVNQV
                     LHVDRKYRQVIFSASGRETGRNPYHQYLYAVGLDDRRIRLLTSNEGNHDLSVSPDGKW
                     FVDNVSTLDRPTRSLLRRVSTGTSLLELSRADIGGLDSMGLKLPETFTATGRDGQTVL
                     YGAIWKPSRFDPAKKYPVIDQSYTGPHTFMYPRNFISGLARSNQALAELGFIVITVDG
                     MGTANRSKAFHNVSYKNMGKNLEDHRLAILQLGSRYSWMDTTRVGIFGHSAGGFDAGH
                     AVLEYPDFYKVAVASSADHDFRMEKDWWPEMYMGWPVDSTYHQVSNITMAGNLKGRLL
                     ITHGGIDENVNPSATFKLAEALIRANKEFDMLILPSQRHGYQGIFNEYFTKKKWNYFV
                     EHLLGAVPLWDIDMKGD"
     gene            complement(25558..26577)
                     /locus_tag="PKM27_00665"
     CDS             complement(25558..26577)
                     /locus_tag="PKM27_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNR05790.1"
                     /translation="MDSFTHVFLGGCLGHLALGKKAGRKAVWWGALADTVPDFDIFAG
                     PFVHPVEALLIHRGFTHSIFFAALFPLVVGPLLARLYRKDRIPWWQWAGLLALGTFSH
                     IFIDSLTTYGTGLLEPFCDHRFTYATLFIIDPAFTLPMFVGFCLLLFKRFSLPFKNKV
                     ARWALILSAIYLAWTFYNRSHFEGLFRDQLQKQNIVYRDQFLSPGPLNNILWGTVARS
                     DSGYFQGFSSYFDTPGPVHFNFIPAQKQLAAPLAQNKEYRLLEKFALGYSCISILDDG
                     LPYFHDLRFGTMHGWAPDPGDFTFSYPLQKDPADSSRLVKSAPWGKAGFEGFGGLWER
                     ILGRN"
     gene            complement(26604..27866)
                     /locus_tag="PKM27_00670"
     CDS             complement(26604..27866)
                     /locus_tag="PKM27_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR05791.1"
                     /translation="MNKRDFIKKLSGAGLLSTGFLAQMEKLVAGIAHRPPAELAADED
                     FWAKVRSGYRLKPDYINLENGYYCIMPRQIQDAFIERVKEVNFQGSWYMRNHRFEDNI
                     EIRKKLAALVGTSYEEVVLTRNTTESLDTVISGYDWRPADEAVMAIQDYGAMLDMFKY
                     QARRFGLVNNRVSVPLHPADDEEIVSLYESAITPRTRLLMVCHMINITGQILPVRKIA
                     DMAHRHGVDVLVDGAHCVGHFEFNIPDLHADYYGSSLHKWLSAPLGAGLLYVKREKIA
                     KLWQLFGDTGFPDDDIRKLNHTGTPPVHTDLAVHDAIDFYELIGAARKEARLRYLQHY
                     WTSRVRDLPHIDLNTPADPARACGIANVGIKGMNPADMAKTLLDKYGIFTVAIDYANV
                     HGCRITPNVYTSTAELDVFVAALKELKN"
     gene            complement(27927..28643)
                     /locus_tag="PKM27_00675"
     CDS             complement(27927..28643)
                     /locus_tag="PKM27_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNR05792.1"
                     /translation="MCGRSSLTKTEKELEERFQASFYSEDLERYNPLPNFNVAPTHVL
                     PVITNQDPRHFRPMRWGLIPFWAKDPKIGYKMINARVETVQEKTFKNALEKRRCLVPF
                     DGFYEWKKVGKKKVPYRITVEGGKLFAMAGLWETWKSPSGEAVESFTILTQPSNKKVG
                     AIHDRMPAILLPEQEKLWIDPSLSSSEALQLIIPYPDEATEIYRVSERVNRVGENDPD
                     LVRPVKENLEGAYDLFSENE"
     gene            28792..29757
                     /gene="ftcD"
                     /locus_tag="PKM27_00680"
     CDS             28792..29757
                     /gene="ftcD"
                     /locus_tag="PKM27_00680"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNR05793.1"
                     /translation="MLECVPNFSEGRDKQIIARIRQGIESVAGVKVLHTDMGFDAHRT
                     VITFAGKPEACLEAAWQSVRIATGLIDMRHQKGAHPRLGAVDVFPFVPLQGSEMTEAI
                     DCARILAERVGRELRIPVYLYGEAALRANRKNLAEVRKGEYEGLNEKLKLEEWKPDFG
                     PTVFNEKSGALITGARKVLVAYNINLAGGDAALAQKIARQLREKNGGLKAVRAIGWWM
                     PAFNCPQVSCNLVDLDLTPLHLVFDTCCRLAEEMGTRVTGSELVGLVPVKVLTDAADF
                     YNQGKTMTEKEKLEFVKTKFGLSSVKPFDPSKQIIEKALGGWQPG"
     gene            complement(29779..30369)
                     /locus_tag="PKM27_00685"
     CDS             complement(29779..30369)
                     /locus_tag="PKM27_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05794.1"
                     /translation="MIYIKTTAFFLMWMLAAPLTGQGLNFHTGVFSTIYTDEQWPGRN
                     PRHQGYHFGFDVVIDDGNYFLMPGLYFLKTSLFPVDLDLSKPYFEYTDLKSIKLPFQV
                     GSYVYKNRFADLKMHGGIAANFLLGIDENPQIVVEDFNEIHAGLLAGMSFRVLFMTAR
                     LSYEHGLSRIYKENAPSGIEDKSRSNVLSIILGVHF"
     gene            30454..31629
                     /locus_tag="PKM27_00690"
     CDS             30454..31629
                     /locus_tag="PKM27_00690"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNR05795.1"
                     /translation="MFPDLSYIFHYLFGTPPDGAASIIKTFGLFLTLAFLASAWILYL
                     EFVRKEKQGLLHPRPIMINPADAVRPADILINALFGFFIGFKVLYAYHHMDEMKTDAV
                     ALLLSLKGNMAGGLIGALLFGLLKYRDYLKVKKQDSKPMEVLLYPHQQVPDITILAAV
                     SGILGAKIFALFEGEQSWSSFVRDPVKAFLSGSGLAIYGGLIVAFITVFYYVKKKGIK
                     PIQVMDAVAPALIIGYAVGRLGCQFSGDGDWGIVNLNPKPSWFIFPDSWWAYTYPHNI
                     LNEGVPIADCSYRHCMQLAQPVYPTPLWETFFSFVIFGILWFLRTRIRIAGILFFIYC
                     FLNGVERFFIEKIRVNEKIHIGSLSLTQAEIIAILLMITGLVGSFLLWKRHKPSVAS"
     gene            31646..32602
                     /locus_tag="PKM27_00695"
     CDS             31646..32602
                     /locus_tag="PKM27_00695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR05796.1"
                     /translation="MTAVDEYRAWAELKGNALPLFLRPWWLDAVCGDSRWNVALVKNH
                     GGEPIAALPFFQFYKRGIHVIALPFLTPYMGIHIDYPEGQKLTSRYDYEKQVLDGLID
                     QLPDTPYLQQKFYPSLTYGLPFFWKGFRLDWRYTYHLSLQEEEKTIWNQMEGAVRTQL
                     KKTSGQVEIVESTDPALMHKLVTMTFRRQQKKMPYSRDQFQRLYESVQNQGAGKILLA
                     VLPPSQEPVAGIFIVHDRDTVYNLVLGRNHLLDPGGSIHSLLWKAIRDHIGRYRIFDF
                     EGSMLEGPEHVFRSFGSLRIPVLQITRYRNRLWKAALALAGK"
     gene            32640..33902
                     /locus_tag="PKM27_00700"
     CDS             32640..33902
                     /locus_tag="PKM27_00700"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNR05797.1"
                     /translation="MPVTGLNARTIPLTFVPLPVYPMDILIKNKVLATMAGLVAHNRA
                     GLLEANQQDLDAGNLDDLTLYDRLKIDDRKIGAMTQSLEETLALPDPVGIEKYHYHHP
                     QGMEIYNRSAPFGTILIIYESRPDVTVEAAALAFKAGNKILLKGGKEARNSNRFLVSL
                     WHLALAECGLDPDHILYLDYTREQTFDLLKSSAGKIDLVVPRGGDQLIEMVKTHASAP
                     VLVSGRGNNFIYVHDKADPAMALEIILNSKLQKISVCNATDKVLIDRQLPGLEAFSKQ
                     LAAALVYEEVSLLADQSLSGLGSLYPVQIPDQENIWYEEFLARRILLGTVSGLDEAVQ
                     KINKYSGGHTAVIITADQEAAAGFMEAVDAASVIHNASSRFTDGGQFGLGAELAISTD
                     KLHHRGPLGLNQLVTNKWYVYGNGQIRK"
     gene            33915..34682
                     /gene="proB"
                     /locus_tag="PKM27_00705"
     CDS             33915..34682
                     /gene="proB"
                     /locus_tag="PKM27_00705"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNR05798.1"
                     /translation="MVNKRKRIVLKLGSTTLTRGTKLISRGKLEDIARQINQLKKENE
                     IVIVTSGAIAAAKHYIELHRGNPVAVKQALAAIGQPVLMRFYQEVFNDYDLKIGQCLL
                     TYRDFENDLSQSNTLATINTLLDNGYIPVINENDTIATDEIKFGDNDKLGALTAVLLK
                     ADLLILASDIDGLYDADPRQDPKARLIHTVKDLSEIEHLQNGGLSSLGTGGIKSKIEA
                     ARICRAHGIEMWIVNGGKDNFAVNALEGKITYTGFPV"
     gene            complement(34688..35290)
                     /locus_tag="PKM27_00710"
     CDS             complement(34688..35290)
                     /locus_tag="PKM27_00710"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNR05799.1"
                     /translation="MKSFFQKGLIEAGVDEAGRGCLAGPVYAAAVILPPGFRNPGIKD
                     SKKLQAEERDRLRSIIEKKALQWAVASCTPAEIDELNILWASVRAMHLALAQLSIPPD
                     LILVDGNRFKAYGEIRHQCLVDGDALYLSIAAASILAKTHRDEYMRRLHEQYPHYGWV
                     NNKGYATPEHKSALLLHGECEHHRKSFDWNQPVQLELFED"
     gene            35368..36660
                     /locus_tag="PKM27_00715"
     CDS             35368..36660
                     /locus_tag="PKM27_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR05800.1"
                     /translation="MLKRPPFLLFADAKGLTYEDKSLYAMGRSAWDAGEVPLEEWIEL
                     PEGGQLYTLPDRQGLGIDIASGEVKVCEKGRAVAAFVPPAYTVLRLSPYLTLPEASPL
                     PLYCYSAVGWYGGKIYVAATRIEKDIRQEAAGYDEKKISRGIAQLTSAYPNNRLVRHL
                     AENCAMRYCCPAARNYFLGRWECPVPVSPACNANCLGCISYIPESEVFQASHDRLAFK
                     PTAAEIVEYTVPHLETAPYPIVSFGQGCEGEPLLMWETIRESIVQIRKFTSKGSININ
                     TNGSKPAAVRALCEAGLNSIRVSMNSARETLYTRYYLPNNYSFRDCLESLRIVREYGG
                     WSSINYFVFPGVTDTPEEYQALCDLIDSTGLSMIQWRNFNIDPDFYLHKLNIPPDEPF
                     LGILNMMKKLRKRYPDLKFGYFNPPRERMFNYETDFAH"
     gene            36787..38223
                     /locus_tag="PKM27_00720"
     CDS             36787..38223
                     /locus_tag="PKM27_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05801.1"
                     /translation="MIKFWCFVFALGLGVTARAQKVTVSQEITLRNDLSYDLVGKIDS
                     HFILFRDRGTKYEASIYDQELKLKTEKEIILADKTCQLLASLATPQSFFIFYGFRHRG
                     DYYIQADQLNGECTIISSDTVKIFENVILNPNIRFARSEDRTKIIFFTDEKDHEMQVA
                     LYDLTAGRTVYDRKIYFKEIFLKEDFRSLLVSNAGEMFLILEKNNYRSRKEKHVFEVH
                     CFYPTAEAARMFVIPVFRFVSFHSKFAYDNVNKQLVGGGIYSQKNLNRAEGVYYLKHS
                     LQIGEEERLTTYAFESRFDEEMTRRSRDKNEFSQLEVSEIALRQDGGALLIMEIKKEF
                     ERRSVYGSGRPLNNPGGFRNYMLVDYYYEDMALFSINADGSLMWSDILHKKQYSHDDD
                     GVFSSFFLFKNPSRLRLVYNDEISSENTVSEYVVQADGQYQRKSVLSTEYVRLQLRFK
                     DAVQVSHSSFIVPSQRSMRLNLVKIEYL"
     gene            38511..40709
                     /locus_tag="PKM27_00725"
     CDS             38511..40709
                     /locus_tag="PKM27_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNR05802.1"
                     /translation="MAISRFKALDLAQNRPMPAVKLPDQKITEYYGSNTFNDDVMKSL
                     LSPDAYKKINEAILTGSKIDRESADEVAAAMKSWAVSKGATHYTHWFQPLTGSTAEKH
                     DAFFDIDFKTGKALEKFKGSALVQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAFIM
                     ENLGGTATLCIPSVFVSYTGEALDYKTPLLKAIALVDKAATAVMKEFFDRDVKRVIAT
                     LGPEQEYFVIDEALFNARPDLMMSGRTVFGHAPAKGQQLEDHYFGSIPPRINAFMVDF
                     EIEAQKLGMPVRTRHNEVAPAQFEVAPTFEELNLACDHNQLLMDLMNKVAARHKLKVL
                     FHEKPFAGINGSGKHNNWSLGTDTGINLLGPSSKPKENLRFLAFFVNVIKAVYDNSDL
                     LRASIASPGNEHRLGANEAPPAIMSVFVGNTMNRVLTEFEKNKEITLEKGDNVYLKLG
                     VNKIPSLLLDNTDRNRTSPFAFTGNKFEFRAVGSSANCAQAMTVLNTMVADQLFKFKA
                     DVDKLIEKGQKKELAISEVLQGYIKASRKIIFEGNNYSEEWVKEAAKRGLGNIKDTPS
                     ALEVYLRKPSVDLFERHGIYTAKELHARVEIEWENYIKKVQIESRVLGDLAINHIVST
                     ALKYQTQLVDNVRGQKEIGIPAKVYQPTVETIKKISEYIGKIQTLVVDMTEARKKANA
                     IEDAHKRALAYNQKVKSFFDTIRYQIDKLELIVDDDEWPLPKYRELLMVR"
     gene            complement(40935..41939)
                     /locus_tag="PKM27_00730"
     CDS             complement(40935..41939)
                     /locus_tag="PKM27_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNR05803.1"
                     /translation="MRQYAFGRRLIFSLVLATSTLFYFGCKNEANITTPAALPVKLKV
                     PVFDKDSAFAYIQTQVDFGPRVPGTEAHKRCGDWMVGRLKACGARVIEQPFTGKTFSG
                     ISFQARNIIASFNEKVRPRIILAAHWDTRFIADQDPEMKNKNTPLPGADDGGSGVGVL
                     LEIARQLQANPIPNLGIDLLFFDAEDQGGEGSDPESWCQGAQHWALHKHTAGYQAKYG
                     ILLDMVGGKSPRFTKEGTSVQYASAFVDKVWTLAQRMGYGNYFVNEVSPAIIDDHYFI
                     NRMTNIPMIDIINRPTNNEFVAHWHTLEDKIENIDREALKATGQVVLAVIYREANGDL
                     "
     gene            complement(41954..43450)
                     /gene="cysS"
                     /locus_tag="PKM27_00735"
     CDS             complement(41954..43450)
                     /gene="cysS"
                     /locus_tag="PKM27_00735"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNR05804.1"
                     /translation="MNQELFIYDSLSREYVRFEPLHEGRVGLYVCGPTVYSDVHLGNC
                     RTFVSFDLIYRYLRQLGFQVRYVRNITDVGHLEGDADEGEDKVSKKARLEQLEPMEIV
                     QRYMNGFHEMMEVFNTLPPGIEPRATGHIVEQIEMVKTILANGYAYERNGSVYFNTQK
                     LIQTHDVYGKLSGKVMDELQNESRALKKQEEKDHPADFAIWIKADERHLMHWPSPWSE
                     GFPGWHLECSAMSTKYLGSRFDIHGGGYDLKFPHHENEIAQNIGCCGHSPAKYWLHAN
                     MLLLNGKKMSKSDGNTISPAQLFSGQSPHISKSYAPMVVRFFMLQTHYRSTLDLTDEA
                     LQAAEKGYKRLMEAVRSLRNLGTEGTRLQASLDEQIRAELDSLYTEMNDDFNSPKVLA
                     VLFDLVTRINSFKDGHLDIQQISSQTLDRLKKETALWVYDILGLKDEEMSNGQGKQMD
                     EIMKLLIDLRQQARARKDFALSDQIRDRLGKIKIVLKDSKEGTTWTHE"
     gene            complement(43514..43678)
                     /locus_tag="PKM27_00740"
     CDS             complement(43514..43678)
                     /locus_tag="PKM27_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05805.1"
                     /translation="MIVKTSSGYQVLSEKGKPLSKDNLTLKQAEQRLKQVEMFKHMKP
                     KGKSQAGKKE"
     gene            complement(43684..44130)
                     /gene="smpB"
                     /locus_tag="PKM27_00745"
     CDS             complement(43684..44130)
                     /gene="smpB"
                     /locus_tag="PKM27_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNR05806.1"
                     /translation="MEIKNRKAQHEYFFRQSFEAGIVLDGAEVKSIREGNANLNDAYC
                     YFRDGELYIRNFHIAPFKQAAGWSVHDPLRERKLLLKKNELKKLRHRVDEKGMTIVPY
                     RMYLSERGLIKMEIVLGEGKKAYDKRETIRAKDNRREMERMDKGKL"
     gene            44197..44997
                     /locus_tag="PKM27_00750"
     CDS             44197..44997
                     /locus_tag="PKM27_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNR05807.1"
                     /translation="MMFLTRIDRIRIRVFILLAVSVCLYPACRHHESTPDSQTRAVTL
                     KQKDLILPDRYNVAFLVMNGVYNTELTAPYDVFQHTQYRENIKAMNVFTVANTHDLIR
                     TFEGLYIKPDFDYTRDKLPKIDVLVIPSAEHHTDSDLNDTVMIRWVRKTGENAMFVTS
                     HCDGAFLLAKAGLLDGVSCTTFPANVKTFKDMFPQVRVYENVYFVHDKNVITSSGGAR
                     SFESALYLVQLLYGKVVADKIAKGLVIDWDLNTVKKVVIHQGNGYSTL"
     gene            44994..46148
                     /locus_tag="PKM27_00755"
     CDS             44994..46148
                     /locus_tag="PKM27_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNR05808.1"
                     /translation="MSRLILLVFLFGPAGMLTGQNPFDIKKPDASKIKPAVKKEEVKK
                     TEAIDPGAATQKKTTHDSLPVAVQTRPAEIVPAPVQKTGRTSANIFDIKSGGNPNEVI
                     LNKPSQSPEGTRPPFPSSRDRSAETAGVDTSTGSIPPSSEATGDPNGRPGGVDLDVLQ
                     KKLKTFGNNTTDARGAISKNAWFLIYVFLFLLAAIAINFNRSYPASLFKATYNQNQLR
                     MLFKDAFKGNHMMVFGILYFLFVVNAGIFIYQTFQIFRIAPLSLLVALGIVLGIYLVR
                     HLVLYLMGKIFPMEKETMFFNYTIGIHNLTAGLGLMLINILLSFVDPASGKTLILVGI
                     CLIGLLYIMRQVRGLLNNLPIIVSRKLHFIIYLCAVEIAPWMLLVRYFSL"
     gene            46206..46979
                     /locus_tag="PKM27_00760"
     CDS             46206..46979
                     /locus_tag="PKM27_00760"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNR05809.1"
                     /translation="MSSTAKTSPEKFKRIKTILVSQPKPEHSPYANIEKKYNLKIDFI
                     PFNQISPVTEKEFRKSRIRPDEFTAVVFTSKTAIEHYFRICEEMRIKVNPDMKYFCLT
                     EAIGNYLQKFILYRKRKVFVGTKSIEDLSAYFNKHKNEKFLLPSSDQGAKEVLDYLLG
                     KGLKVTEAIMYRTVSCNLSHLKDIKYDVLVFFSPLCLKSLYENFPDFKQEETRIAAFG
                     TQTSKAVEDLGLKLNIKAPAPDVPSMSMALEKYLTVSNK"
     gene            46981..47784
                     /locus_tag="PKM27_00765"
     CDS             46981..47784
                     /locus_tag="PKM27_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNR05810.1"
                     /translation="MPELAGRLISTGLISLSIPVFFLCKTAPDSPQAPLPLKAARPSV
                     SSPATDTLNYVMGKFDPGSNERFAPVEQKYASREGMFLRKETLEAFVKMAKAAETDGV
                     ELKILSATRNFTSQKSIWEDKWTGKRLVEDGQNLALTTPDPVKRALKILEYSSMPGSS
                     RHHWGTDMDLNALDNSFFAKGEGKKIYDWLVAHAGKYGFCQPYTAGRPHGYHEEKWHW
                     SYMPLARPLTGFCERHLKNEMISGFLGAETAKQINILQHYILGINPDCR"
     gene            complement(47797..49881)
                     /locus_tag="PKM27_00770"
     CDS             complement(47797..49881)
                     /locus_tag="PKM27_00770"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HNR05811.1"
                     /translation="MYKFSVQYCVLTLILLTGWFPSCNKPVGTDREQDLADESNTSDW
                     LAYGRTHSERRFSPLTDIDTSNVSRLQVDWFLDLPRDHALVSTPLVIDGILYFTGTGN
                     VVRAVDATNSKALWTYDPEIAKEIGNHRKVGWSHSRGLSYYRGKIFLATWDGRLQALD
                     AGSGKLIWSTRTFDLNKPWFITGAPKAFKGKVVIGNGGTEQGPSRGYVTAYDAETGQQ
                     AWRFYMVPGNPADGFENEAMRMAAGTWTGEWWKHGGGGNAWHGFTYDPELDLLYIGTG
                     NGSPWNRKIRSPGGGDNLFLSSILALDPDTGEYRWHYQTTPGESWDYNSNMDIVLADL
                     MLEGIKTKVILHAPKNGFFYVINRETGKLISAKPFVETSWASEIDLSTGRPVEIAAAR
                     YVQGPGYVTPGPHGAHSWHAMSFNPITGLAYIPSIHDGNVYSDQGIDLASWQAVDFKG
                     GIGVGLTTPEKLPRDYPASLQAWDPVTQKLAWSVPQQELWNAGTLTTAGKLVFQGRAD
                     GKFLAYHASTGEILWTFDAGLGISAPPITYKLKGRQYLALLVGWGGAFAGVGNKALGW
                     EYGKHMRRLIVFSLEGKKALPPLPPPYFPVPLDAPDFEVNDSLAKAGQSLYGKCSGCH
                     GPGLLAAGMAPDLKASAIALNLDLFRLVVQKGERLNMGMPAYPDLTDEQLLSLMHWIR
                     KKGNEILYPKEL"
     gene            complement(49912..50565)
                     /locus_tag="PKM27_00775"
     CDS             complement(49912..50565)
                     /locus_tag="PKM27_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HNR05812.1"
                     /translation="MKVIVTGSTGMVGEGVLHECLHHTAVESILVINRKPCGIRDPKL
                     TEIILPDFFDLSGIKAQCTGYDAFFFCLGVSSVGLSETEYTRLTHSLTLYLASSMVAL
                     NPGMVFCYVSGAGTDSTEKGRMMWARVKGRTENDLQKLPFKKVFLFRPGFIEPGAGLK
                     NTLRSYKYLFWLVPIIRWLAPNRICTLKEIGMAMIHAVLKGYDKKVLEVSDIKKLAR"
     gene            complement(50579..53596)
                     /locus_tag="PKM27_00780"
     CDS             complement(50579..53596)
                     /locus_tag="PKM27_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNR05813.1"
                     /translation="MTKTGFISLLFCLGILSCKEQNKPAPGGFPPEQALTTFELADGF
                     QMELVAAEPLISDPVAMDLDEEGNIYVVEMHGYPLDKSGSGVIKKLTDSNGDGRPDKS
                     TVFADQLVLPTGIMRWKKGFLVVDVPHVLYLEDSDGDHRADIRDILLTGFALTNPQHI
                     ANTPVFGLDNWIYLAHQGAVNPKVFPEEFGDTGSAVLYPAKTGGPVLPADANGRNVRF
                     KPDESLLEMMAGESQYGQTFDPWGHQFLTSNADHLFSEIIAARYLQRSSTLLVADATE
                     NIPDHGNACEVYPITQNPEHQLLTDVGVITSSCGVTWYDGGLFPDSFSNVTFIAEPVH
                     NLVHADRIADRGASFTASRVYEQKEFLASRDAWFRPCQFYIGPDGALFVLDYYRQIIE
                     HPEWMSDEVNKSGTLYNGSHQGRIYRISPSGTPPMDWCGKLSLGSMNGEQLAALLDHK
                     NKWWRRQAQRLLLDKQDTAAALGIRTILRTGNMSPAGTVHALWTLRGMNNLVSEDLKP
                     ALEHPHPRVRENAIRLAEDGGLVQSLWNTLVSMNQDPDSKVRFQLACTLGSFNHPEAK
                     RAIRKILFMDIEDRWVQIAVLTAAGSFGPDMVVEMVRQLGTRETSGRKQFFEQLGAGI
                     ALKGEPASIIQTMGLATQSDKEPSAWWKAALLKGLYSGIRTNKPDPSSVKIYRDALPG
                     SFQANQPAEIRAAVVQLFTVLGLPEGEQKSTMALRARKFISDPASPAGLRKDAIKILG
                     LNQDTSDVLLIQGLLSPGETDEIQEAALQAFTDIAPQAARAYSLEHWKNLTPAVREAA
                     LQLLMKSPPGMSALLKAVETGQIQKSSIGWPRTVRLMNSYTDSIREYSRRLLGHPEES
                     RNAVYEKFRAALSMEGDPVQGKSLFMQHCAICHQVNGALGKSIGPDLATIRNREAGFI
                     LADILDPNRSIADEYQTWTIEKKNGEKLSGIISAQTQSSLELKDAAGTATLIARTEIR
                     LLESEPMSLMPVGLEGNLTVEDMAGLLAFLKGK"
     gene            complement(53698..54801)
                     /locus_tag="PKM27_00785"
     CDS             complement(53698..54801)
                     /locus_tag="PKM27_00785"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNR05814.1"
                     /translation="MKTVHSVLIFLFSIYAAQAQMDLPPVGGNPRAILSEEVGITGIT
                     LTYSRPDVSGREGKIWGTLVPFGFSTFSFITNGNTAPWRAGANENTTIRFEHDVLVEG
                     RPVPAGTYGIHMAVWPDSVLLILSGQHDAWGSFYYEEKNDVLRVIVKPLKHDQHVEWL
                     KYEFIDHKPNSCTVALLWEKWIIPFKIEVDVENIVLTRLREQVTSQKGFNSNNLIQAA
                     QYCLNNNINLDEALLWSQRAVGGFQGQRSYFSLRNLAAVFEKLNRMTEADSVMTEALT
                     MATAAQYTSYSRTLISQKRTGRALDIQQASLQKHGDIYLVNLGFMFVYSARGDFENAL
                     KYGQKALTQAASPGARAQLESNLSRLRENKDIN"
     gene            complement(54828..55337)
                     /locus_tag="PKM27_00790"
     CDS             complement(54828..55337)
                     /locus_tag="PKM27_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05815.1"
                     /translation="MNRMKFIRSMAAGSLGLAVPSAMLQSQNNPKPASLPVEKVKEFV
                     GVCHSDLDKVKALQAEFPNLVFASWDWGGGDFESGIEAAGHVGRKDIVAFLLEKGSRT
                     NIFLLTMLGQTAWMKSYLELYPSHLYMKGPHGFSLLHHANKGGEEAGELAAYLQQLGL
                     KETRFNLYS"
     gene            55478..55678
                     /locus_tag="PKM27_00795"
     CDS             55478..55678
                     /locus_tag="PKM27_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05816.1"
                     /translation="MAEPIRAYNVKTKEKNVEMKDAVITKTARGGYMAQGNDGKGNKL
                     TSLLSEAKALQAIQDGVAKKGW"
     gene            55860..57170
                     /locus_tag="PKM27_00800"
     CDS             55860..57170
                     /locus_tag="PKM27_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HNR05817.1"
                     /translation="MILAVFGILLVLVLLITWAKIHPFIAFLMVSILASLLLGVNPGQ
                     IGASVQKGVGDILGPVFSVIILGSMLGKIIASSGAAQQISESLVTLFGQKNLAWALAL
                     TGFIVGIPLFYNVGFILLIPLIYSLGQRFQLPLVYLAVALCSSLSVTHGFLPPHPSPT
                     AIVPMMNASLGQTLIFGLIASIPVIFLAGPLFAKSLRNIQGSPLASLKVKIFEKAEMP
                     STGISFFCALLPVVLLALGTWFTSIFEKNSPAFEISSLMADANIVMLLSLLLAYYFLG
                     MRRGDSLGKVMQEGIESTKDVFPILMIIAGAGVLKEILISAGISTQIASIMKTSSFSP
                     LVTGWFIAAVIRLSLGSATVAGLTAAGLIAQGFDPAVVNPNLMVLSIGAGSLMFSHVN
                     DTGFWMFKEYFNLNIRDTIKSWSLMETIVSFAGLIMVLIMDIFV"
     gene            57195..57677
                     /locus_tag="PKM27_00805"
     CDS             57195..57677
                     /locus_tag="PKM27_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNR05818.1"
                     /translation="MKPEYQPEKIRDRFLKNQQAYKKFLARAPLKKGIPKLAGLAQEV
                     YARHDCLSCAACCKNYSPRFKAPDLKRISKYLGMKETELQDLYLKRDEDGDYVMKTAP
                     CAFLGPDHRCSIYEKRPSDCQRFPYIDEDVFLKRKELTLKNLSFCPIAQDMMEKLMER
                     "
     gene            57733..58134
                     /locus_tag="PKM27_00810"
     CDS             57733..58134
                     /locus_tag="PKM27_00810"
                     /inference="COORDINATES: protein motif:HMM:NF021996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HNR05819.1"
                     /translation="MKRKLILLLFSFSILACNRDEVEGDPIPADLTTLLQGSFESAAH
                     PTSGTVKLAKDGAGKKYLVFENFKSDPGPDLRIWLAEDKSATNYSELSTTVPSGNFKI
                     DVPPGADTSTRTYVLIWCKAFTVLFGSAQLK"
     gene            complement(58163..58414)
                     /locus_tag="PKM27_00815"
     CDS             complement(58163..58414)
                     /locus_tag="PKM27_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05820.1"
                     /translation="MRNPDPIHEYHRMVNMFLDHQLNDEENQKVLHQAQENPEFQRVL
                     RTEQNFRDLIKKNVVQRPHVTSRLIDNIRNKISSQGDGK"
     gene            complement(58444..59043)
                     /locus_tag="PKM27_00820"
     CDS             complement(58444..59043)
                     /locus_tag="PKM27_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR05821.1"
                     /translation="MNEEERKRYDRVFEKELLPHIDALSTFAFHLTLNEDDADDLVQE
                     TYLKAYRFIDKYLEGTNAKAWLFKILKNAYINEYRRKSKAPAHVDYEDIIGYQDSEES
                     AIGEYTDLREELFNTVMGDEVTNAINSLPVDFRTVILLCDVEGFTYEEIAKILDVPIG
                     TVRSRLFRARNVLKDKLKAYAETMGFSDKRKSRSRKLEE"
     gene            59634..60137
                     /locus_tag="PKM27_00825"
     CDS             59634..60137
                     /locus_tag="PKM27_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNR05822.1"
                     /translation="MKLKTGKSQVELLSRHGLGNIYYDHHTQQRSFQATKNIKSGQIL
                     YRFGPKQIQANPTYLTVQVGEGQHMVLDPEFLAAINHGCDPNVFFDVDHGVLRSVRDI
                     KAGEQMVYFYPSTEWQMIQPFDCTCGSPKCLGYIQGAHAIEPELIQRYHLSSYIQSQL
                     KEVPTLV"
     gene            60162..61310
                     /locus_tag="PKM27_00830"
     CDS             60162..61310
                     /locus_tag="PKM27_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05823.1"
                     /translation="MKSFRRFKKLTGNRKIKVWVLFPYLKTNDEQLDYYYDFNQSVAE
                     YTRVFEKIKMPWTWQPVTLDHYREVIEGLQQELHEGEFFPVVLNLCDGDEINGTPGLS
                     VVKALEYSGLVFTGADHFFYEITTSKISMKEAFDAHGVSNAPWKTIQASNPSPAGIFK
                     RLGKPLIVKPAVSGGSLGVGIKNVVNTPSEMKTEVLKLFEGYRGWEFGAGGVIAEKFI
                     TGPEYTTLIVGSYDRPEDLIYFAPVERVFHASLPDHEKFLSYERLWEVYENESSMPLQ
                     GNFYEYKSPPFEIQDALEAISLSAYQSVRGKGYGRIDIRMDQTSGKLYVLEVNAQCGL
                     SEDENFTSIGAILKYSQKSFDYLIREIILDALIRFEVEAGVRANVLVG"
     gene            61350..62777
                     /locus_tag="PKM27_00835"
     CDS             61350..62777
                     /locus_tag="PKM27_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNR05824.1"
                     /translation="MKVCVLQPDYSASSVDYKYYDPPRDLSRLLPGHTVDHVFLNKLH
                     TYGQLSGLQHAGYDIFVNLCEGYLDWDIPSIDVIYALESLKLPFTGPTSRLYDPSKEL
                     MKYVAYTEDILTPRYALIERPEDIPKAVEKLQYPLFVKPAKAGDSLGVDDHSLVANPA
                     QLLEKARALLLEYPSLLVEEYIAGREFTVLVAADPANEKQCRTFKPVEYVFPSGFAYK
                     TYALKTSELHLEANVPCQDPELENRLRLAAQRIFRGFGGMGYARLDFRVNDKKEIYFL
                     EINFTCSVFYSDGYEGSADYILRFDGIGQAGFLQHIIREGISRYKAQLKKYTMKGDSI
                     NGFGIYANVHLAAGEVIFNNEARGHRIVTKQFVDKNWSAVEKDLFARYAVPLSGHLYI
                     LWDDDPSVWAPQNHSCDPNTGYDGLNVIALKDIPVGTELTLDYATFLDETMEPFACQC
                     GSDQCRGWIKGTPGNSMEKRESLRD"
     gene            complement(62778..64142)
                     /locus_tag="PKM27_00840"
     CDS             complement(62778..64142)
                     /locus_tag="PKM27_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05825.1"
                     /translation="MKRRIVFWGTNTENEKVLVALQLDPEQNIVHEWIFPQAHVTEEF
                     ANTMMRDWRIGKELDFPESAVHSEKALTASESLLSPGLKPDKEDIILRAQTEWHFIVL
                     SHKLKQGFENEYRDLKEKTDKLLKYDNELWEELKTFWDRVQNQVREKNLFKEHAQILR
                     DGTNALFEQLKKLRKAVDEEFDRTSRDHVNQFQKKLDEISHKLSNNLSLQPLFSELKE
                     LQNALKGIEMNRNDRSNIWDQIDKLFKQIKDKRFGHKDSREGSGSDRLSRRYEGLIEA
                     LQKMESSIKRDYNDLEFEKRKVANSEGQLEAQIRQAKIKMIEERIRSKEEKLHDMRST
                     REDLEKRQEQEKQREIRRQEKLKLEQAKHAVEDRIKEEIKKAAEARKDDTRLEKAAEA
                     ISGSPKPVEPHDNILDAIGTTLGETLEDVVDTVRAVSEVVGDKIQEFTENLKSDKPSE
                     DKNA"
     gene            complement(64195..65214)
                     /locus_tag="PKM27_00845"
     CDS             complement(64195..65214)
                     /locus_tag="PKM27_00845"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNR05826.1"
                     /translation="MKVACVGATGLVGTVMCQVLEERDFPVTEFIPVASEKSAGNKFL
                     FKGKEYKIHTLAEAVAKKPDLALFSAGASVSLEWAPRFAEVGTVVVDNSSAWRMDPSK
                     KLVVPEVNGEVITAGDRIIANPNCSTIQMVVALAPLHKAYGIKRLVISTYQSVTGTGV
                     KAVRQYDREHSGEQVPQEEKAYKYPIFENVIPHCDVFLDNGYTKEEMKLVNETKKILG
                     DPGIRVTATAVRVPVKGGHSESVNVELERDFEMDDIIKLLSQAEGVQMMDDLATFKYP
                     MPLYAKGRNEVFIGRLRKDESQERTLNMWIVADNLRKGAATNAIQIAEYMLKKELLPL
                     EAETV"
     gene            65336..67057
                     /locus_tag="PKM27_00850"
     CDS             65336..67057
                     /locus_tag="PKM27_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNR05827.1"
                     /translation="MRPLHSLLAGLFLFVCGIRVREFFLPDIHRNKTRVSPDPVLPFD
                     LLISEIMADPSPVVGLPPYEYLEIINHSGRTINLHGLTLSFESRAFRIDRDDLLLEPG
                     QYFMFCEPDAFPALSPFGPVLGLEAWPGIRNSHGTLSLYHQNQLIHRVSYTDEWYGDG
                     RKKNGGWSLEMINTSNWCDQIKNWTASTDTTGGSPGRKNAAADSNFKVEPALDSVVVS
                     GDQKIIVYVNKDIQEPSVSDLVFSPPMDETRIVPGDDLQSFMILLPAPLLSGQLYQIS
                     IRLRDCAGRNAGVHSSILTKPEKAERNDLIINEILFNPEGRGSDFVELYNRSGKVVFI
                     KGWVLENKMNRQKKTIGTAAYLLPGGYLVLSPDPVDIAFRFKKVQPDVMIAQDLPALA
                     DGSGHLTIYSQGGLLVDSVYYEETYHHPFLNSKQGVSLERVNPEAGADRTNWQSASGL
                     EGFGTPTRKNSASVSVAGSEYFQLKYSLFSPDGDGYRDQLEIEYRLPRNGYLGRVYIY
                     DDRGGLVKKLVNNLSFSQQGSIEWDGRDEAGMNCPTGIYILAAEALDMQGNHLQKKLA
                     AVLSRPG"
     gene            67128..>67702
                     /locus_tag="PKM27_00855"
     CDS             67128..>67702
                     /locus_tag="PKM27_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05828.1"
                     /translation="MKRIKQLLTYQWVEGGIILSMILITLLLLFTPNRATLAGLSSHA
                     FEAVLLFLGSAMGFMILDRPKIMFTCLGMCAILCLYLKKTSNSSIQSLFRQADIDFSL
                     CQILINSGADEYENISRRLLASKADILNIQELTPDWALVLSDGLSENYPYRAEMNRVD
                     PYGMVVYSKYPILKTDTLHFRDTMVNYDIPVL"
     assembly_gap    67703..67802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            67958..68458
                     /locus_tag="PKM27_00860"
     CDS             67958..68458
                     /locus_tag="PKM27_00860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR05829.1"
                     /translation="MSSNLHTSKELNPLNEALAVHCKPFAELVAAELYDILRLRQQVF
                     ILEQSCLYLDTDGYDEAAHHLMIYNKQKDLVAYSRLFGLNMPYAGYLSIGRVVTSPRF
                     RGLSYGRILMRHSIEQVRSIHGPHPIKIGAQAYLIGFYSSLGFRSTDQRYLEDGIPHL
                     KMILDL"
     gene            68455..69291
                     /locus_tag="PKM27_00865"
     CDS             68455..69291
                     /locus_tag="PKM27_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNR05830.1"
                     /translation="MKRNLILNTMHNLIGLFILITGISCNSSPKTDDPPPYKLVWNDE
                     FEYDGLPDSTKWSYNVGGHGWGNNELQFYTLKRLENARVENGLLVIEARKEEWEGNPY
                     TSARLISAQTGLDKPYGWKYGKVEVRAKLPEGRGTWPAIWMLGDPQPLQWPDDGEIDI
                     MEHVGWDQGRVHASIHCKKYHHSIGTQKTANISVPDCSQQFHRYGVEWDAEEIRVSMD
                     DQVYFSFKNEHHSYESWPFDNKMYLLLNIAVGGFWGGQQGVDENIWPRRMEIDYVRVW
                     QK"
     gene            69366..69854
                     /locus_tag="PKM27_00870"
     CDS             69366..69854
                     /locus_tag="PKM27_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05831.1"
                     /translation="MKGLYFIWISMAWSGLTWGQQFARQVFILSDEFSKEACEVIAAC
                     DCCASDLIFLNKKKFALVDRCLHHDTYLRGKYALKGKSMTLDFSPEAVSLRYDENTQL
                     INKEKKQMSMSPLILQIGRCGQDEVTLGHPTIREYKYGSRRKGINEKEILRQIKKSKP
                     WK"
     gene            69968..70744
                     /locus_tag="PKM27_00875"
     CDS             69968..70744
                     /locus_tag="PKM27_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNR05832.1"
                     /translation="MKNLKEKIRSGEALHGCWINLGSTVSAEIIGRAGFEWVLIDLEH
                     GSGNDLAMYQQLQVLEGTPATPLVRTDELSRSKIQRILDAGASGIMFPQIRQAAIADE
                     VCRMMYYPPKGTRGMAKMVRATGFGKNATEYIHTLENNLIGVVQIETPDALEQVDRIA
                     SLDAVDVLFVGPSDLSLSLGVFGQLDHPVYQKAIRDVALAAKKHHKATGVLLLDLAEY
                     EMYFQLGYRFLACGADATFVARGAEAMAQALSKKRSGDQV"
     gene            complement(70848..71669)
                     /locus_tag="PKM27_00880"
     CDS             complement(70848..71669)
                     /locus_tag="PKM27_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05833.1"
                     /translation="MSYKPIPIRSGVWGYRGTDALFLDLTGTDIPALAAFDLAYLNGD
                     HHVQTLIAGGKTGEPSSFLLGLGDGDNGLLGNQRDPVELKAVYYPITPPHLTRTSERS
                     ISGSSSNRQNSIVSIPRLAADEVFVLRGFEFQGTQGGNHHLRKVGVRYHSIRSEIRVD
                     FVDDSPQDDRYAYIIRYFILKRGDHPRGLILRGVYSQSFEFTDSARIPKTNRGNALLA
                     GFNFEFKDGDHHLRRIAVDVQPTNEFGVVFTDSQTNHGVKASVDYVVLDWSGAYR"
     gene            71806..72303
                     /locus_tag="PKM27_00885"
     CDS             71806..72303
                     /locus_tag="PKM27_00885"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR05834.1"
                     /translation="MVVIVMGLPGSGKSYFASKLAGTLGGEYISSDEVRMKRLSRRTY
                     SEEEKEMVYRVMGQMMSEALSLKKPVVLDATFYRESLRNYFEEKAAAFGEKIMFIEVT
                     APEHLIEERLQKPRTMSEADHPVYLKLKAAYEPLKREHLTLVSSNHNISEMLSEALAY
                     LHSVQ"
     gene            72300..73310
                     /locus_tag="PKM27_00890"
     CDS             72300..73310
                     /locus_tag="PKM27_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05835.1"
                     /translation="MNREQIDILLKAGRFPHPSLIETHISYVILGNSLAYKFKKEIKY
                     SFLDFSTLKKRKFYCEREVLLNNRFSKGVYLGVVAVRQDKKGITVDGPEGKIIDYAVK
                     MKRLKSSNQMHRMLEKKQVTRAHIQALASTIRRFHLHTDVIRAPFRPRTFSSRFNDLL
                     SVSGFVKSVLGSSWDELIKNAVRLSDRFLKLHHDLFIKRIKMGFIRDCHGDLHSRNIF
                     LYRKPILFDCIEFNDDFRKMDVLDEIAFFCMDLEAEGFYLLSKAFTDDYFHKDPGAFG
                     PREQMLFTYYKSYRANVRAKVNALRAMKAEGAQRKKYLDEVKKYLKLMDGYLETPIPK
                     FQ"
     gene            73322..74413
                     /locus_tag="PKM27_00895"
     CDS             73322..74413
                     /locus_tag="PKM27_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05836.1"
                     /translation="MRLQYLLRWGTCLMLTALGTGAKPGGPANSKKIEIRGIYGSPEP
                     FWKKDLHLDQLGVNAVFMHDGAINEKIMSRARLEGLKVFAEFATLNGKNYVDQHPEAW
                     AIDQHGKKVEAATWFMGVCPTEPGFRRYRTDALKNLLRKFDLDGVWMDYLHYHAQFED
                     PEPILPETCFCDHCLSLFQAATGLPLPGGNTAEKADWILRHQEQIWRNWRCTIIAGWV
                     RDFKKILKEEKPGALLGIYHCPWNDEDFNGARRRILGLDYDLLREHADVFSPMVYHGR
                     MGKDPEWVKENISWFTHRLDIQPDQYPKVWPIVQAHNDPGVITPEVFEKVLRYGAIGR
                     SSGVMMFTSYSVAEDEEKTAIMKKVYTGW"
     gene            74587..75279
                     /locus_tag="PKM27_00900"
     CDS             74587..75279
                     /locus_tag="PKM27_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05837.1"
                     /translation="MSLTRFWCGLLIPAAFLLVSSCKSTTVLLANFNNDHIGSPPAPN
                     QATGTVSVTSGGGSVIVTSPNDTIKSKKWCGISHPFNNSDPTELTGNFTQFGKGKYGL
                     LASLYIPVTEVVTVQFEASKQSPLPFGPFLHLDFMPEGDVRIDDGTHRFGQFPRNQNF
                     VLSVNLNITDVAATAEITLLGANASGNTTVQISQNVLPLARHFGAVKFWVGFQHRATF
                     FVDDIIVTRKNQ"
     gene            complement(75311..76432)
                     /locus_tag="PKM27_00905"
     CDS             complement(75311..76432)
                     /locus_tag="PKM27_00905"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HNR05838.1"
                     /translation="MKYFSLLMPLLVTRFMNPLIAQDDYFLIIGTYTTAPSDGIEVYR
                     YNSAKCEFQYLHKVAAQNPSYLALSPDQKYLYAVTETGNPNGGGVSAFRFDDRQGNLE
                     LINSQSSAGNHPCYVSTHKNGKWVAAGNYSSGNLVYYPVASDGSIGNPTNIQHSGSSI
                     VKGRQDAPHVHAAVYSPDYKYLMVPDLGIDKVMIYKLQPDGNIISKKPGSVQLQAGAG
                     PRHLDFHPNGKWAYLMEELSGKVTAMNYKKGKLKVIGTVNGHPADYPGPYGSADIHVS
                     PDGKFVYCSNRMESNTIGIFKVNPVDGSLSLVGHQSTLGKTPRNFTIDPTGNYLLAAN
                     QNTNDVVVFKINRETGLLTDAGKRLSVNKPVCLKWAVKK"
     gene            76629..>78115
                     /locus_tag="PKM27_00910"
     CDS             76629..>78115
                     /locus_tag="PKM27_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNR05839.1"
                     /translation="MKRYFLYFFFSFSWYVLPAQIRFTQVKTLSGTLTDIGHAGDGSN
                     RVFITHKTGTITILDQAFNTLGTLLDLSGQLATDSEKGLLGLAFHPDFENNGHFFVNY
                     NPANTNHTVIARFTASTPSANTTVSIGTLKIILTITEAANANHKGGDLAFGLDGYLYI
                     ATGDGGGGGDPQGSGQNGNILLGKILRLDIDTDQAYLVPPSNPFVSDPGIRDEIWALG
                     LRNPWRISFDRQTQDFWIADVGQGAREEINFESGGFAGGRNYGWNCREGFLSYAGCTG
                     NFTQPIFDYGHCNPCSNTAGTGNSITGGFVYRGTKPANTPMRGYYICGDYVSRHGWMI
                     KYNPGSPVESKTISRLTPGGVTTFGEMENGEILAGQSNGVLGLIESTETALPFKLSRF
                     QAHWIHPRVELRWSNHTETDLAHYTVEKSKEGLRFLSIAQVIPKKEESAPNDYIYYDE
                     QIESGESYYRLKLTGTDQSADYSDIVRVRVPDNENSLAFYRASTR"
BASE COUNT        19086 a        19917 c        19248 g        19764 t
ORIGIN      
        1 tttaatttgt taataaatca attttaaact ttgacacact tttctgaata ctctctcctc
       61 agcctcagcc ttaaccttaa cctcaaccta aacctaaacc tcagcctcca ttaattccta
      121 tttcctcctc ccatatctcc accaccgtct ccctgcttaa ggtcatcgtt tttgataccc
      181 cgttttttct ggggggcgtt aaaattcatc ttgccccact gatagctgaa attgatgccg
      241 aaggaggcaa agggaatttt aaaattggat tcctggataa aattgtcgcc ctggatgctg
      301 gacttgaatg cgcggtaacg gttgaaggga gttaccgtat tgaaaccgag ggagcctttt
      361 ttcttcagga tctctttttt caaaccgatg gaatacatgg agaaggaggg agtggtgccc
      421 tgcaaggacc tccgcggcga attgaagatc ccgaacaaat cggctttcag tcccttggtt
      481 ttaaaatcaa aggaggacat gatgaaggca ttgtattgga tgccggcgct gaaattttcc
      541 tgggtcaggt cgcttttggt attatacgac ccgatattga cattgcccct cagggtccac
      601 tttttggaaa gattgaccga actgaaagca ttcactccat aggaatagtt gtcccccgca
      661 tttttaaaag ttgcctccga ggtgccgtcg ttgcggatct ccgtaaggtt ttcgatgatg
      721 tcatcggtgt gtttagcata aaaggagagg ttgaccatgg accctttcag gtaagtggtc
      781 agagcaagtt ccagctggtc ggtgagctcc ggccgcaaga ggggatttcc caccgtgatg
      841 ttgcgggtat cctgggcatc ctggaaagga ttgatgaaaa acaggctggg gcgctggatg
      901 cgcttggaat agcccagttt gatcgtcctg aaatttttca tctgcttgga gatagagata
      961 ttgggcagga aattattgta gtcattggag aaaggatccc cggcccctgt cgtaaaccgg
     1021 ccgtgaatct tggtattttc ccaacgcacc cctgcgatca tgctgatggt tttgcttaat
     1081 ccggccgtca tggaagcata accggccatg acatcctggc tgtatttgaa cacactggtg
     1141 cggccggcat cgggcaggta aacctgctga tcgggatcga aattaaaata attcaggtca
     1201 ctgttgatgt cccgcaacac cgatttggcg ccgacttcca ttttaatttt tgaggtgaag
     1261 ggatgggtat aatcggcctg gagggtgtat tcgttattga tcccatcgtt gtcgcttttc
     1321 tggttgaagt tgtattccgg gttattttcg gtgataaaaa tattgtcggt gaggctttgg
     1381 ttgttggacc actgaaaggc aaggccgaat tcctggtcct tctttttaaa ggttcttctg
     1441 aaatcggtgc tccagtcgaa accgccgcgg agaatgtcgc tttcgttgat cctgccgtaa
     1501 agcagttttt gattcaggat ctggtcatcc acggaaacat tggtcaccga attgcgatcc
     1561 atgccaaaac cattggcccg gaaggtgctt gaaatgctgt tgaacgcatt aaaatcataa
     1621 tcggctccga tattgccgtg gtagccgatc cgggaagtgg tattgacacc cagttctgaa
     1681 gttttgaccg ttttgtttcc gacaaaattg gtccggtcca aagagttggt cccttccccg
     1741 ggccatcccc agaaagcgcc cccgcccccg tttaatccaa agcggccctt gcgcaccccg
     1801 aggttgagaa ataaattatt ctgccgggtc cccagggcgg agttgatgct gccgctggtt
     1861 ccttccgcgt ttttcttttt ggtaatgatg ttgatgatgc ccccgcttcc ttccccgtcg
     1921 tatttcgccg aaggattggt gatgacttcc acgcttttga tctggtccgc cgggatcatt
     1981 ttgagggcgt ccgccacatt ggaggaaaac atgccgctgg gtttgccgtt gatgaggatg
     2041 cgcagattgg tgcttcccct caggctgacg tttccgtcga ggtccaccga caacatgggt
     2101 actttctgca gtacgtccac cgcactaccg cccgcggagg tggcatcttt ttccgcattg
     2161 taaaccattt tatccaccct gttttcaatc agactggcct ggtcttccac ggtgacggtt
     2221 tgaagggcca cgccttctgc ggccaggagg atggtgccga cctgggcatc gggttttttc
     2281 ggggtcagct caatattgct gatggtcttg gtttcataac ccagaaaaga aatcacgatt
     2341 tcgtatttcc cgttggatac gttctccagt ttaaatccgc ctttttcatc ggtgagggtg
     2401 ccgttgatgt ctttggtgct tcccaccctg cgaagggcca ccgttgcgaa ttcgacgacc
     2461 tcccggctgg tactgtcgat gatattaccg gtgatctttc cggtgactgt cgggccggcg
     2521 gcacctcctc ccatggggaa ttgggcattg gtccgggtgg ccaggcagct aataagtgtc
     2581 aaaaaaagaa gggtctgctt tatcatattg ccgcaaaatt gaatcaatcc ggcaatgcaa
     2641 acccgtaaac tataccattt cgtcctattt gtagatgaaa agaatgattt cttcgataaa
     2701 gacaaaaaac aacgattatg actgttttat aatagattat atattaaaat aatatttaaa
     2761 gcaagtgatg gtctatatac cccatcattc cattgggaaa acggagggat ccgtcgggtt
     2821 tttaagctgg tcaagactac ctatttccag gcttttctcc cggtccggca gccaggcgag
     2881 aatctttgga tttttaaggg tcagtctcgc tttaagccgt tggttttcaa tcacatcatc
     2941 aaacccgatc tgaaaaattt gttgagggcg gaccacgcgt accggaccaa aacgctggag
     3001 agtattgttt tgtatccaat aatgcaggcg ggtgaccaat ggatcgggga ggccctccgg
     3061 gatcagcctg acaacgggta ccagcgtctg atcctcattg tagattccaa acgtgcattg
     3121 ataaacggag agccgggcag gaatggcctc ggcgtacatc aatacggcgt tgacctgccg
     3181 gcctggcggc ttgatccgga tccagggtat ttgttccggg tgatagggtt cgtccaggga
     3241 cttcagaacc cagccggtcg gagattcaag caaaggcagg tcttcaaggt tttgcaaaac
     3301 tttataagtg attacccgga taccgaaccg gaccgggtcc ttaaacaact catcgagttt
     3361 ttgtttccgg ctggataagg aatggctctt taattcctgg tattccaatt gcaggagatc
     3421 aaaagccaaa aacttccagc cggattctcc tttccttttt tcccctttga cctgcgattt
     3481 tacaaccggg gggccggtca gtattccttc gaggacggtg ccctccggta aaaacccaaa
     3541 aaaagagagg gggtcttcga tcggagaagg catatattct tccccataag cccagaaatg
     3601 tacttctgca tttattaaat gaacctgcac ccggatgccg gaaacaaagg gttccgcgat
     3661 ccagttagcc tcctgaactc ctgatcctga ttccggatct tgggcagata tttcgcggaa
     3721 tggaataggt ctggcgcggg cttccgtttt ttgccaaacc gggtcaatta attccctgaa
     3781 actttccggt ccgagggact gccgattcaa cctgaaactc accagggcct gctccacccg
     3841 atactgtttt gaaaggaacc gggacaggaa cccctgttcc aaaagaggct tataggtacc
     3901 aaggaccagt cgatgaaaaa aaaacaattc aggacctgat agtttatccc aaaaagaaag
     3961 ggtcagggtg cgcaggttct ccaatttgtt tttggactgg tcgatctcat ccaaaaaacg
     4021 gcgaaggcta tatcccgaac tccggatttg gggattcccg atgatcaggc tgaaggtttc
     4081 ggaccagtcg cccgtgacgt cgtgactttc ttttatcaac cattcaggca gtttggtgta
     4141 ttccagaagc cattcgagga gctgggatct tcgaaccaaa ggtttgattt tataagcatg
     4201 aaggaggtcc agggccagtt tgttctccat ttcttcattc tttcgtaaaa attcctccag
     4261 ggcaaggatc cggttttccg ggaatttatc cagcaacagg gtccggtaca gggaggcaaa
     4321 atggtcaagc atgatatcag tcctccccgg ccctttcctg attttccgca gaacggtttg
     4381 cttcaaaacg gtaaccttcc ctgaccgcct cagcctcgta ccccagggtt ttcagccatt
     4441 ttgaataagc ctcggtgtac ccatgggtaa cccagatttt ccgggcaccg caagccctga
     4501 cgacctggtt gagcgaggtc cagtccacat gatcggacaa ggcaaaacca tggttagcgg
     4561 cctggttctt cctaatggta ttcagggtca tccaaccgga aaccgtggcg gtgaccggtg
     4621 aaatcaggct tctcacccag ggattgttga tttccgaagc cggtgtgagc gtaatgccct
     4681 tttggatttc tttttttgtc agcctggaag ataatggaat ggtcttcctg agtttgaggc
     4741 cggcctttct caatatatcc gtggtctgtt ctacagaacc ctgtgtatac accgggatct
     4801 ccggggggat caggtggatc agtcgttggg acttacccag agcataggcc agcaataagg
     4861 tggatttgcc ctgttcctgg tttttttgat accaatccaa tatttctccg aggatcaccg
     4921 gttgcggctg ccattggtaa acaggaaggg caaaggtgga ctcggtgacg aggtggtggc
     4981 agggcaccaa ctcataattt tcacagagcg catcggcttc cgttttataa tccccggtca
     5041 cgacccagat ctcgtcccgg taagctaccc tcacctgggc cgagcccggg atatgaccgg
     5101 ccggatgaaa actaaaacgg acgccgttga tatccagggg gattccaaaa tcatgggttt
     5161 gtatctggta aaaactaccc aaccggtagc ggagaatggc cgccgtgagt tgggtacaga
     5221 gataatactg gtggccggca atcgcatgat cgctgtggcc atgggtgatc agggcccggt
     5281 ggaccggccg gtgcggatcg atataaacat cggcccgcga acagtagatt cctttcgggg
     5341 taaagctgag cagtggttca cggtgcatga ctgaataagc ggttatggcc gtttgtatta
     5401 cctttgtgcg acttaaatta aatacatttt catgaaatcg ttgccttgga tcataatggg
     5461 tgtcctgact cttgccgttg caaatctata ttttaaagtt tacgacaagt cgggttccgg
     5521 ccaaatcatg accggctccc cgtccgaagg caaccctctc cggatcgtat acgtcaatgc
     5581 cgattccgtg ctggagaatt ttacccaatt caggaaggaa aaggacgccc tggacaaaaa
     5641 acaactggat atcgataccc ggctgggggt caaaggccgt gccctcgaaa acgaagtcgc
     5701 taccatccag cagaggattc agagcggcct gcttacccca aaagaaatcc aggaccagga
     5761 acaaaggctg ggcgcaagac aacaagccct gctggcggaa agggataaac tggctaagga
     5821 aatcgtggat gaaggcacgg ccatcaatga ccgtttgcag aaagtattga tcgaagccct
     5881 cgaaggcctc aagaaggaaa aaggttacca ttatatcttc agttatgtac ggggcgggca
     5941 aatcctgctc ggaaatccgg cagatgatat cactggggat gtgctggcca tattgaataa
     6001 gacagggact ggggctgcga tggatacttt gggcgggaat cagtaatccg cattcaggcg
     6061 caagtcgttg tccacggatt ttttcattca gaggcaagcc cgttgttcga cccggtcttc
     6121 agagttttgc cctgatcccc accatgactc ccctcggcat tcccggcctt aaacccgccg
     6181 gcctccgggc tacggcgtag acctcattaa acaaattatt tatgctgccg aataagttca
     6241 ggtgccggtt cagggtatga tcggcggcga ggtcaaagac caggttgtgg tcaatgcgtt
     6301 cggtttcgat ccatctcccc gacccgggga cggtgcgcat ggggctgttg tatttggcac
     6361 tcagatgaat actgtaacgc ctacgctcca atcctgcatt aaaaacgagt tgatgtgcgg
     6421 agatatatgg catttcatct cccttttcca ccttgcccca atcttcgttg gcggcttcaa
     6481 aggcgctgtt gaattcaccc cgggtatagg tatatgccag gttgaacggc atgctccagt
     6541 ttttgcctga gccggtcagg atcagcccgg cttcggcttc cacaccataa atcaaggcct
     6601 tgcccgcatt gaataggtca ccaacccctc cgcccccgga tgccgcaagg tccgaaccca
     6661 gcagattttt atagtcgttc agaaaaacaa cggcctgacc attgaaaggc ctccgggtaa
     6721 accggctgcc gatttcataa ttgatggcct tttccggcag ggagccgtcc gcgacacccg
     6781 gaggcgaaaa cccacggtga acgcccccga agaccatgga gaaggaatta aattcatact
     6841 ccaacccgat acctggaatc cagactccca ctttattgac cgcctctttt aacgaagacc
     6901 cggtgcggga agggtcctct tttccaaagt cctcccggct gagcgtgatc ttttcatacc
     6961 tcatgccggg caaaacttta agattgcctg atttcagggt gtattgcaca taggacgcgg
     7021 tagccgcagc tttgctgatc cggttgcttt cagaacccgg attccccttt tggtggagtt
     7081 tcatgacccc gttatccatg gcataagcat cgtcccactg gaagcggtct tcttcgtcat
     7141 ggtgaaaccg gaacccgaat tcaatatcct gctccagggc gccatccgaa atatttattc
     7201 cgaggatccc ctggatcccc tttgaaacat attctctgtt gttattccgt acaaacaagg
     7261 catcggcatt agggctggtc aggcccttaa gcaggttgta ctgctgagca taggtgtccg
     7321 ggtcatccag gatggctgca atggccgttt ttgatccggt gccgtatttt acctgatcca
     7381 gtttatacca gtttcgttta aaatccgact tgtaggccgt gaccgacacg ttgaggaaac
     7441 gggcaggaat catgctgtac tttatggaat actgatgttg tttggtccgt atttggtcca
     7501 tttgagatcc atgataccgc cggttgggag cggcttcaaa atcaccctcg gtgagaccga
     7561 gataggtttc gtcggccttg ccctccgact gcccgatttt gatcaacacc gcttgataaa
     7621 ttttcgcccc gggcgcagta ttgaaccgca ctttggccag ataatcctga ttgagaaaac
     7681 ccgtcgggcc tccgttgtcc agtttcttaa atccatcggc tttgttctgg aaggtttcca
     7741 ccacaaatcc tccgtgggta aaagattgcc cggcagtggc atgcaccact ctccttccaa
     7801 aatttccgac agaaagattt acccctgccg cgatctcatg gggtatgggc gtggacagga
     7861 aattgattgc gcccccaacc gtaaacgggc cgtacttgat ctggctggat ccctttctga
     7921 cttcgactcc aaataccctg cctgctgtag gaaaataata ggcggcagga gccgcataag
     7981 gcgccggggc ggccagcacc ccatcttcca tgagggtaat cttactgctt ctttccacgc
     8041 cggaaccccg cataccgata ttgggccgca atccaaaacc atcctcttcc tggaggttca
     8101 cccccgggat attgcgcaag acccggtgga tgtctgtgta attgaatttc tgcaactcgg
     8161 gcaatgaaat ataatgcgcc gatccgggaa tgtccttgac gtataaactg cctcccgtca
     8221 tcgtctgccg gccaataatg atctccggca attcatacag gccctccctt aaccggatgt
     8281 cgagggtggt agcggttccg acctcgatca cctggctcac aggttcaaac cccacactgg
     8341 caaaccggat ggtatacacc gccggtgcca ggaccagccg gtagtccccg tctgcattgg
     8401 tcaccgtatc cttatccgtc ccttccgcat atacgctgac aaaagccagt ttattgccgt
     8461 tgaggtccag gacccgtccc tccaggattc ctgaagactg ggccagaagc gttttaaaca
     8521 tcaggatgcc gagcaatgag aaacaatacc gaagacccat aatctgaaat tggagcgcaa
     8581 tgatatctat tatttagatt taatccaaat aagaataaca atttttggac tctgtgattg
     8641 atctcaggag ccttccctcc gccgggctac ccgatccaga ccgcctgggg ttttagtgaa
     8701 gcatgccata caatacggca gccgccaccg caccaaggat cggacccagt accggcaccc
     8761 agctataagt ccagttgctg ccttgtttgt cacgaatggg caggagggtg tacataatac
     8821 gcggaccgag gtctcttgcg gggtttatgg cataccccgt tgccccgcct aagctcaaac
     8881 caatacccag gacgatgagg gctaccggca aggcatccag ggccccgagt ttgtattccg
     8941 gggatacgat gtgcaggacg gcaaagacca gcacaaaggt gcccaccgct tccatcaata
     9001 aattgtttaa ggtattggga atggccggcg aagtacagaa ggtggccatt ttggatcccc
     9061 ggtcctcggt gctgtcgtag tggtcccggt ataccaggta aaccagggta gccccgatca
     9121 ttgcgcctaa aaactgtccg atcatgtaat taacagccag gagccagctg atttttccca
     9181 tgacggccag ggcgatgctc acggcggggt taaggtgggc acccgaatga ggggctgcca
     9241 ccatgacgcc gacaaaaacg gctatggccc agccaaaagt gatgacgatc cagcccgaac
     9301 cattggcata ggtccgtttt aaattcacat tggctacgac tccgttgccc aataaaatga
     9361 gcatggcggt accaatacat tcagcccaga aagcggacat ttattataat taaggaatga
     9421 agaaatgaat aattaaagaa tgaaggtaat gaaggtaatg aaatggtcat tgattggctt
     9481 gggcgatctt gcgcagctct tcttcaataa ccgttttggg catccagata ccctggttca
     9541 tcacgcctga aatggtttta atattttcga tccgctcgac cggattttgt tcgagaagaa
     9601 tgaggttggc cgcctgaccc acggcaatat ctcctgactc tttttccgtt ccgaaaaacc
     9661 gggaaacctc cgaggtaccg gcctggagga tcttccaggc agggatgccg gcttcagccc
     9721 aggattccat ttcgtggtgg atggagaaac ccggcacatt catcacctgg ggggcatcgg
     9781 agcccagaag cagcctgacc ccggattggt gcaagtccag gagcagtttt ttgcggatat
     9841 ccaggtaggc cttgtatttt tcagccgtgt atccggcgga tttaatgaga ttggttttgc
     9901 tctgatgcca ggcaaaacgg gtccgggaag gcatatatgc catctcttcc tggcgcatca
     9961 tggcagccgg atcctctgga gaaaaccagc gggtaaacag ggtctgggta gggaccagcc
    10021 aggtcccctt ttgtttggtc aggtctgcca atcctttgat tttattgaca tcggcctggt
    10081 cagtgaggtt aaaaccaaaa aatccgtttt gattcaggtc ggccagcggc ggagccatcg
    10141 cttcaatgta tccgtcgaga tgatcgatcg taagctggcc ggactggata gcatgcctca
    10201 cccccacttc aacgggtacg tgaccggcga aagggatgcc cacctgtttc gcggtccggg
    10261 tcagggcatc gtaaacctcc accttgattc cgggatgtat ttttaaaaag tcataacctt
    10321 ccgattggta ttgtttcacc agtttttctg cggtggacac atcgggtacc gaattgccgt
    10381 tgaaggacgg actggaggtg tacacgaccg gcatattggt aatgccctgt ttggccagtt
    10441 tttcctttaa attcaaatgg aagggttgtc ccaacatccc tctcagggtg gtcacaccgc
    10501 ggcacaggta taaaaacagg gtttgtttta ccatcgacag gtccccgttg gcctccggcg
    10561 ccggtatgtg cgcgtgcatc tccgccagtc ccggcagcag gtattttcct gcgccatcga
    10621 tggccaggga agaggtcggg attttaaccc tgcccgcccg gcccagctct ttaattttgc
    10681 cgttttcgac aatcacgatc tgatccgtca atatagtctc cgaacccggc cggatcacat
    10741 tgaccccggc gaatactatg ggttgggagg gggccgtcag ggacagaact tcctgggcat
    10801 ggattgccgg tttgcccatg aacatcgtca aaacaaggag gggccatttt ttcatagcat
    10861 taattttttc ttcgttcgta tattttttca atttcccggg ccagggccca gtccttttcc
    10921 gtgaccttat gcccggcgga atgagtggtc aggatgatat ccacgcggtt ccatacgttg
    10981 tgccagtcgg ggtggtgatc cagtttttca caggcaaagg ccacttcggt catgaaggaa
    11041 aaagcctggg caaaatcggc gaattgaaag ctggccctca attgatcttt ctcttccttc
    11101 cacatggggc tggttttgat ttcctaaggt aggccatttt ttagtctttg ccaatcgggg
    11161 tttgactgcg ctaatcccta catttgcaac aacaaataca ttactgcttg tccgtaatta
    11221 aaaaacttgc cggtgaaacc gctttgtacg gactcagcag tattctcgcc cggctcgtca
    11281 attttatcct ggtccccctt catacggcag tgttcaagga aaggggcgat tacggggtgg
    11341 ttggccagat ttacggatgg atcgccttgg gagtggtttt atacacgctg cgcctcgaaa
    11401 ccgcctattt caggtatgcc agccaggatc cccgctccgc ctcttaccgc accatcaaaa
    11461 ccagtattgc aggcattgcc ctcatgggtt cttccctgat cttcctgttt tccggaccca
    11521 ttgccgtctt cctggatatc ggcggttacg gtttcctgat ccgcctggcc gcgggcatca
    11581 ttgccctgga tgctttgtgc gaactgcctt tcgcccggct caggctggag aaaaggcccc
    11641 gccggtttgt agccatcaaa accattaata tcctcctcaa tgttgcactc aattttttct
    11701 ttttatggtg gttgcccgga agaggagccg ggtcctttga tgaaagccaa ctggtttctt
    11761 atgtcttcct ggccaacctg atcgccagca cagtcaccct gatgctgctt tcaaaagaat
    11821 tcctgacgga gggattcgct ttggaccgga aagaaatcaa aaaggtattc aactactccc
    11881 tgcccttgct gattgttggt ttggccggag tgatcgacga cgccatgacc cggcaatttc
    11941 tcggctggct tgccccgggc accctggctg aaaaacagat ccagatgggg gaatacaatg
    12001 cctgtgtcaa attcgcagta ttcatcagtt tatttgtcca ggcttttcgt tatgccgcgg
    12061 aacctttctt tttcaaacac atgaaccatg aaaacgcccg gcaggattac agcaggatca
    12121 cgacctggtt tacccgtttt ttgatgctgg gttttgtggt ggtattgctc taccttgatt
    12181 tttacaaaaa cctcattgtg cggacgcccg aatacctccg tgccttgggt atcgtcccca
    12241 tcgttttgct ggccaatgta tgtctcggca tctattataa tgtgtccaac tggtaccgcc
    12301 tcaccgatcg cacccaatac ggcatgtatt ccgctttggt tggggtgggc cttacccttg
    12361 gatttaacct gttgctcgtc ccgaggtatg gatatatggg gaccgcctgg tccatgctga
    12421 tctgctattt tggaatgacc ctgattactt atatctgggg gcagaaatat tatccggtgc
    12481 cttatgaact cggaaaaata gcaggagccc tgttttcagg tattgccatt tacctggtga
    12541 gcttgttttt gaaaaatcaa tttaactggt ccccctggtc tcagtttctc gtaaatagtc
    12601 tcctggtagg agtttatatt tttattttct tcggaaattt gatcagggag caggtgaaaa
    12661 gggtcagggt ttcctgaaat ctttttatta ggcctcgcta caacaaattc agatccttcc
    12721 tccagaaatt gacccttact cccgccccga taaacctgct gatatccgaa gcgtcctcga
    12781 ggtcctgccg gtaatacccg ttcaggtcga taaaatagtt gtgaaacaat tcgtaagata
    12841 agttgaatcc caggtgacgg atattccgga gccggccctg ggtggttttg ttgtcgtaat
    12901 ccataatgcg ggtggtatag ggctttagca ggtcgccccc gtaattgacc gaaggcatgg
    12961 ggtcatcgcc ccacaggtaa gccatcaggt aagcatgaat ctgccagcgc ggagcgggct
    13021 ggtatctaag atttaaaaga tattctttaa aattggcgcc cagcggatgg gccagggccg
    13081 tgcgctgatg cgtgtaattg gccgtactgt caaaatgcgt ataggtatag ggccttacgg
    13141 aattgtattc ggcctgcagg tccaatctgg gaattccgaa ggcatcgatg tattttatcc
    13201 caagctggcc cccgaattta ttcccccacc agcccttgcc gctgctgaag acttctttgc
    13261 gtacgaattc gtcgatgagc acctggccat aaacggatat tttccggcca aagtcatacc
    13321 ggctgttgat gcccagcatc acattatccg ggcttccgat ggaaccttcg accgagcgat
    13381 agatgatcac cgggttgagg tactggaatt caaaccgatt gtttcggtta aagatgacgg
    13441 tctcaaacaa acccagactc caccgggagg acaactgggc ctgcagggta tgggtggcgg
    13501 tgtatttttt gggcaggagg acattccccg ccgtggaagc cgtcggagtg gaagcgctta
    13561 actctgcaaa caggctttgg taatgcagtt tccacacccg ggcttgcaat ttgagaaaaa
    13621 aatattcctg ggcatattcc gataaaaaca gcgatcgcag gccgtctccc aggaaatgcg
    13681 acccgtgtcc cagctggata tgaaaatgcg gactgagccg gaaacccagg tgagcgttgc
    13741 tgataaaaaa atcataaccc tggggtttgt acagtttgtc cgaaaaatat ttgtaaaaac
    13801 ccgcaccggg caaagccttg tttttttcaa ccttattccg gaaaaaagcc ggaagggata
    13861 actggttttc aaggatcgta ctctgggcgt atatcttttg atccagtccc accctcaggt
    13921 ctgcgccgcg ttggttggcc agcacccaat cgcccgcccc ggtttcccgg ccggcctgta
    13981 cattgaggac cggattgagc cgcaagtaaa attccttttt attaacttcc agaaaatggg
    14041 caggggtcct gtaaaaatgt tttaataccg gcctgctgct gtagcgatag cccatggaat
    14101 ccaggccggt ccctgatttg ctccggacgg tatggccgcc atccatcagc cattcgttgt
    14161 tttcatcaaa caggtatctg atgtccgcct cgtcagcggg gtccagaacc ttcaggctgt
    14221 cccagtaaaa gaccaggtcc acgaggtctt tcctgaaata aggcctgacc gcgcggtgaa
    14281 atccggaagg caccccgtat tttatactca tcctttcgat caggtattcc tgctttgaag
    14341 acagggggat cacgttgccc tgggagatca acggaaacgt ccatgcaaat attgaaaaca
    14401 gacccaggca gatccggtat gaaggaccat gggcctcagg atttgaagtc cagtaatttc
    14461 ccgcgaatgg tgtttttgaa tcgttcttcg tcattaaata aaaatcggac ttccaccggc
    14521 agcacaaaaa tcggtatttt attttcaagg atataatcgc ggtgtatgga caagcgggtg
    14581 gcgtccttct ccgtcgtcag tataaattta ccagggccgg gaagcgaatc aaactgcctt
    14641 ttgatctggc ccaggtcata attgctgaac caatgatgat cggcaaaggg gaaaaggtgg
    14701 aggtatttga tttgggagcg gacaaaggcc tgcaggtagt cggtgttggc tatcccgctc
    14761 accagcaagg catagtggtc cgggttccag cgcaggcgca ggccgggatt aaagataaaa
    14821 tagggcgtgc cgtaatggta ttcactgaaa taaagggtct gatgcggaaa gggcttcaat
    14881 tcttccttga gttcatttgc ttcttccgga gtaaggccgg acgggcattt ggttacgacg
    14941 aggatatcgg cccggcggta actggacctc cattccctca ggcgtcccga aggcaggata
    15001 taatccctgg tgaagggcag gtcataggag gtcagaagga tattgataaa gggtttcaca
    15061 ctgaggtgct ggaaggcatc atcgagcaga atgagccggg taccgggata agatttgagc
    15121 agcagcggaa tgcccagttc ccggttttcc gccacggcta ccggcacctc cgggaatttc
    15181 attaaaaact ggaagggctc atccccgatg cttttcaccg agggggcttg ctggcctatg
    15241 cgaaaaccgg tgctctccct tttataaccc cggctcagga tggccaccgg caggtattct
    15301 ttgagccagc gaaccaggta ttcaatatgc ggtgatttgc ccgtaccgcc tatactcaga
    15361 ttaccgaccg aaatgaccgg aatatcaaac cgtgcgggcc tcaggatctc ctggcggtac
    15421 agcaggttcc ggataaggat ggccaggcca tacagaaggg aaaaaggggc cagcagcagt
    15481 tttacaagcg tattccggat catggtgcaa atttcaggat ctggtgtcca atgctgcact
    15541 gcaggcattt tttttcatcg caatattggg ttttgagctg gatctgtgcc tggctgtgca
    15601 aggcatgctt cggattccag cccagtttta tccaaccgct gatgagggca ttggattcgg
    15661 ccgggaggtc ctggagataa gccatggact tctctttccg ctggaggtca tcctggcttt
    15721 cgccgtaaca aaacaaaaga ggcgccacgg cattgatcag gatatattga atcgtcgaca
    15781 aacccagttt tttaaaccgg ccgctccggc tttccgattt aaaggaataa tggtcggtcc
    15841 aatatccgcc cagggtgaca tccaggctgt ttatgatttc tttgatggtc tgggcagcca
    15901 tcagttttga aaacaaatga tcggtagtat atatgatttt ggccaactgg gcgatccgga
    15961 tcgtcggaaa attggcaggc ctcaacctca ggaacttcca atgcgaagcg ttcatgggac
    16021 ggagatggta tttgttttta aaaaaagaat actccgcctg caaccggagg ggataatctt
    16081 cttcaaacgg gccttccagc aaaccagact ggccaaacaa caaagcctcc acgtgcagga
    16141 gttggtcctt gtgtttccca atcaggtgca gaggagtgca ggaagcgagc aattccatgg
    16201 catccccatt gacctgggta cccagggccc tggccagttt ctgaaaacat acttcttccc
    16261 agttttgacc ctggacatcc agggcctgcc ggacggcgac cgctttggat tcgagccgct
    16321 ccgccataag ccggtgcgac caggattcct tgataaagtc gtttacagta tgaaacaagc
    16381 cctcgcaagg tacccatttt tgattttgca tcaggtcctc gtactgcttc agcatctgcc
    16441 ggtcaacccg cgactgcaat tccaggcagg gaatcttcag gccggcttcg ttaaatactt
    16501 cggcgtccgc agaccagact acatggagaa tgaccgtatt gtacgcttca tcggtctcgt
    16561 gccggtggga ggtccattcc gaagcgttga agtgcatttc gacattgcca gcccaggtgg
    16621 tctgcccgat ccggatgatg ctttgcagga aatcgggccc ggcatggtgg ttccggatac
    16681 cgggttgcag tacctgcaag ggttctcccg aagccgtttt cagcaaggaa tgatcaaaac
    16741 agcggtgctt ccagatataa taaaggagtt cttcagagtg tttcataggt attccaaagt
    16801 tagatccgcg ccgcgaaaag aatcgtgacg tttggcgaca ggcgctggcg acaggactaa
    16861 cggaagcgcc tggaaaacca ataatctttg agcagggcga gcgtcttgta aaccgtcttt
    16921 cttaccttca ttttactggc tccggctttg agatcgtatc tcagcacaag cgggagttca
    16981 aggcataccg gtttgaatcg ggcgagtttc agcagaattt ccaccataca gctaaagccg
    17041 gtttcctgga taaaccggtc accataatgg gcaaatccct tttttaacag ggagacccgg
    17101 tatgcccggt aaccgcaagt ataatccctg actccgggta tatgggcaaa cagggaaaac
    17161 aaaagcctgg ccaggacgct gaattgcctg cgggaccaag ggaggccatg tatccttgct
    17221 cccggccgaa atctcgaagc aatcactata tccgaaccct gtctgatggc ctggaccatg
    17281 gccgggatca ggtccggatg atggctattg tccccatcca gggtgaccac gaggtcttcg
    17341 tcctgcagat ggtcgctggc ataagtcaag ccttcgcgga tcaccttacc cagtccctcg
    17401 ttgaccgcat ggtcgagaca acgaaggggc aggggcccct ggtagtttcg caccacttcc
    17461 atcgtacggt cagtgctgcc atcattgacg atcaggacct ccacctctcc tcctgaggaa
    17521 gcaaaaacca gatcaaagtt ttttaaaaga gggccgatgg aggcttcttc attataaaca
    17581 gggatcagcg ccagaatttt ctgcatgtca aagcctctct tttaaaatac gcaggatctg
    17641 gtccgcgctg tccccggtgc cataaggatg ggccttttgt tgaaaactcc ggtaaaacgt
    17701 ttcgtcttca tacatcctga ccatattttc caggatggtg ttttgttgat tgcccaccaa
    17761 tacggcagta cccgcctgaa tggattccac ccgttcagtc agggtcctca ggaccaggac
    17821 cggcttatcc aggtatgggg cctcttcctg gatgcccccg gaatcggtga caacaaaata
    17881 ggatttgttg aggatccaga ccaggtatgg atagggtaaa gacgcggtaa gcaaaatatt
    17941 gggaataggc tcgagacggt catagaccat ttgccggata ttgggattgg gatgcaccgg
    18001 gaaaacgatt tgcacctccg gaaagcgatt ggcaaatttt tcgatagcgg ccaggatccg
    18061 gtccaggggt tctccgaaat tttccctgcg atgacaggtg accaggatga tcttttttga
    18121 ccaatcgatt tgattaaaat aatccacata ttccgtttca tcctggctgt ccaggatttc
    18181 caggcataaa aaaagcgagt cgatgacggt attgccggtg acaaaaacat cccgggtgag
    18241 accttcggcc accaggttct ccgcagcggt ccgggtgggg gcaaaatgaa aatcggccag
    18301 cctgctgcaa agcagccggt tcatttcctc cgggtatgga ttgaatttgt cgtggctcct
    18361 caggccggct tcgagatggg cgatccttac cttatggtaa aaggcggcca gggaaccgga
    18421 gagcaccgtg gaggtatctc cttgtacaaa tacgaggtcc ggattttcct tttcgatgat
    18481 tttatccagc ctcttcaggg accgggataa aaaggaaacc ggagtctgat ccgggcgcat
    18541 cagattgagg tgatggtcgg gtctgattcc gaaaaaagcg gctgtatcgt ccaataaacc
    18601 cttatgctgg ccggtaaaac aaacaacggg ttcaaaattc tcttccctcc gcattttaag
    18661 gataaggggg gcaaatttta tcatttcagg gcgggtgccg atgatgaaaa gaatcctctt
    18721 taatttgccg gaaggcatag tggccgcaag ataggcccta accgcgactt taagcgctgg
    18781 tagtgccatc aaaggtcaat cggataaaag taggtggttt gagctgacca aaaagtgttt
    18841 gctaaatatc tatttgatca taaaacccat tcagggattt tatatgggag ccagttagat
    18901 aggttattat ttcgaaagaa attatttcac cgtgcaacca gaataatcaa ctgtaaagga
    18961 atagtctttt accgtaagtc tttgaattgc agaattggtt ttgtggtcag gtttataagc
    19021 tgacccggta ccgaggtcaa cagctacccc gatcagcccc catgatataa ttgataaaat
    19081 gaaatttcct gttctcaacg ttttatcaaa tatttgagtt ttgggctcgc aaccttcctg
    19141 cctgagttcc accgacaata aacggttcct tgaaaataaa ccagtagccg atccctgtcc
    19201 aattttgttt ccatccacat aaatttgggc gtttggatga tccttggcca aaatggacgc
    19261 atgatacttg ctgcccccaa acatgacccc gcaccggcta aacaatactg aaatcagcaa
    19321 cagggagaat actccgaata gaaatgtttt catagcatgg tattttttta aataattaaa
    19381 tctaatataa gaattttcag aaatttaata ctgccctttt aaattttatt gaaattttct
    19441 cagggttttt agggataatg atatgttgaa ctgaaccgac aatggcgatg cctgatctgt
    19501 taaaaactca aatgctggtt aatcttgaaa accggtaccg gcatcggaga tgcctgacga
    19561 atttagaact tgataataga aataatccag gcttcggcga agcccaacaa gtaagaacaa
    19621 tattcaggac tagtaagatt atcacagcaa agactttcca ttaagacttt taaccttact
    19681 tccttactgt caatcaacaa attaccatat cttctcaact gtagtgcttg tcacccattt
    19741 actccaattt ttattatagg tatgcacacg ttgcgtttgt ccacccaggc tgtcctccag
    19801 gggtaagcca acattaaccc attcaaagat gcttccgtat aaattatgga cccggctgaa
    19861 tcccagcgac ttcagcttaa tcccaatttt ttcactccgg taacctaccg agcaataaac
    19921 gatgacctcc cggtcttcag aaatactgtc cagcacggtt ttatcccaat ggtcataacc
    19981 gatatgaatt gccccaggga tatggctgac ttcatattcc ggtctttccc gggcatcaag
    20041 gaataatacc ccctctgcgt ccaaggcttc ctcaaccgaa ataaccggga tggaaaagtc
    20101 cagaatggag ctgatctctt tgtcaaattc cggattaagg accaggccgc tgggggaaga
    20161 agtcctgcag gatatgcagg cggcaaccag aactacaaga atccggcaat agaaggattt
    20221 caaattcgag tatagtatgg acaaacttta tctacgtctt cgccgccctt ttcgcccttt
    20281 gcatttcatt ctccccggcc ggccgctgct gttgtttaaa cagttcaaag cgggaaggct
    20341 cctgttgctg acggggataa aaattatcct cgccgtttac atcagccgtc tccaggtagg
    20401 gatccaggat gatgcgtttg acttctttgg aggtgcggaa gactttggtg atttttggtt
    20461 cgggcaggga tttccagacc tcggccgggt agcggaaatc ttccttggtc ccgtcctcat
    20521 actggagttg tacgatcacc ggcatcacca gtccaccctt ccgctcgaaa gtcacttcgt
    20581 aaaaatggtc cccgctgccg agtaattttt tttcatcctc ggacaggttc cggtaaaaac
    20641 gttcatagtc ctgcctggcc agggcgtccg gctcgccggg atcatagctg ctgtaaaaat
    20701 cgcgaagggt ggtgtcctgg tcggaatagt attgtttgat ttccgattca ttccgcaccc
    20761 gggatatgta tttcagcgca tgggtctctt gttcgcgttt catttcggcc agtttgtcgg
    20821 ggtttttggg gtccaccttc agccatttta catcggtcat ggccaggtcg cagtaatcgg
    20881 tgctgtaaaa ccagccgttc cagaaccagt ccaggtccac cccggaagcc tcctccatgg
    20941 tcctgaaaaa atcagccgga taaggctgtt tgaaagccca gcggcgggcg tattccttga
    21001 aagccaggtc gaacaactca cggcccatga ccgtctcccg gaggatattg agtccgacgg
    21061 ccactttgtg gtaggcgttg ttgcccaatt gctggatgct ttcgctgttg gtcatgatgg
    21121 gttccagcag gttctggtcc attttcatat agtcaatgat ctggttggcc gggccgcgcc
    21181 ggctgggata gttgggttcc cactgcgctt cggagaggta ttgcaggaag gaattcaggc
    21241 cttcgtccat ccaggcccac tgcctttcat ccgaattgac gatcattggg aagtagttgt
    21301 ggcccacctc gtggatgatc acgctgatca tgccgtattt gacctgcctg gaataggtgc
    21361 cgtccttttc acagcggccg aaattgaaac agatcatcgg gtattccatg ccgatgcggt
    21421 cggcgttgat gctccaggcg gtggggtaag ggtagtcaaa ggtgtaatgc gaataccatt
    21481 tgagcgtatg ggctacggcc ttggtgctgt acttttccca gaggggattg ccttccttgg
    21541 gatacatgct ctgggccatc acgatgcggc cgtcgctttg cggcacgccc atggcatccc
    21601 atatgaattt gcgcgaagtg gcaaaagcga aatcgcggac attgacggct ttgaatttcc
    21661 aggtcttttt ggtcgttgcc cttttcattt ccctttcttc ggccaccttc tggctggaaa
    21721 tgatcacggg ttgcaccctt tcgtttcggg ctttggcgag cagggcgagc tgttctttgg
    21781 tcagtacttc ggaggcattt tgcaattcgc ccgtggcggc caccaggtga tcggaaggca
    21841 cggtgatggc cacttcaaaa tttccaaatc caacggtaaa ttctcccttg cccaggaatt
    21901 gtttgttctg ccagccttcg aggtcgttgt atacgcacat gcgcggatga aactgggcga
    21961 tggtatacag gtagtttttg tcctccggga aatattcgta tcccgaacgt cctcccaatt
    22021 tcatgcggtc gttgatgttg taccaccatt tgatccggaa agaataagtt tctcccggtt
    22081 tcaggggttt gggcaattcc accctcatca tggtgtagtt gacggttttt ttgagatcgg
    22141 ccccggtttg gtggtctttt acatattcca gcttgaagcc tccatcgaag gtatggtcca
    22201 ggttgtcgat ctgggctttg gtcatgcggg gatccatttt atgggaattg agttcatagg
    22261 tctggctgtt ttcggccatc tggttctggt cgagctggag ccataggaaa ggcaggacat
    22321 cgggagagtt gtttttgtac acgatgactt cctgaccgga aacggtttgt ttttcatcat
    22381 caagttcgag ctggatggaa taatcggcct gctgttgcca ataccggtgg cccggggctc
    22441 cgccggcatt gcggtaatcg tttggcgtgg gcaacaactg gtcgagctgg cggaagggat
    22501 ctttgtttat accggtctgg gcgtgagtgg ccagaaagca taaactgaaa aagaataagg
    22561 taatgaattt cattttttct ggttttggca gtataaaatt agcggttttg cacaaagtca
    22621 aagcttgtgt gcaatttaat tagttaacgg ggaaacccat ttgctagtcc tttattcccc
    22681 ggttaaaaaa ccggatacaa acgtcatttg cctctaccgt tcttcttcag ggacaattga
    22741 gtatttttaa acaaactctt cccttgatga aaaggatcct ttatacctgg ataattctgg
    22801 ccttggcatc cgaattgtcg gcccagatgg aagcccggac tccaacgccc gaagactacc
    22861 ggcgagccgc ctccttccag tggcagaacc tggtcaataa aaaaatcttc aatgtgaata
    22921 tccagccggt ttggtgcggc gacagcaccg gaatgggttt tgtgacccag gaccgtgaac
    22981 ggaaaagttt taataaagtg gtctggggcg ttacaaagat ccaggcctgg tttgaccagg
    23041 aaaaactggc caaagccctg tccgattcga caaaacaaaa agtgacggcc tccgacctgg
    23101 ctttacaaaa tataaactac aaaaactcct cgcatctgga attccatttc cagggcaaaa
    23161 gatatgagct gaaccttctg acccactcgc tgaaaatcct caacccgccc gcctccaatc
    23221 ccctggaatc aaaatccccg gatgggaaat ggatcgcctt tacgaaagat tataatttgt
    23281 acatccgttc cacggccgac ggcagcgaac ggcaacttag ccgggacggt aaaaggcttt
    23341 atgaattcgg caccttttac ggctggtcgg atatcatcga aggggaaaac ggggagaggc
    23401 ccgcccgttt gtttgccagc tggtccccgg attccaaatg gatccagacc tatctctgcg
    23461 atatgcggat cgccaaaaaa atgtacctgc tggactggtc tgtggatacc ttattcaaac
    23521 cccgcctgct ttcttattac cggggctcac ccggggatac ggacatggtg cgtatgatcc
    23581 ccgtgttcta caatatcgaa accggagagg aacaaatcaa aaaccacctg caaagcaccc
    23641 ataccaatcc catcggctac gaatggtcaa aagaaagcgg gatcgtttac caggaaaatg
    23701 ccattcgggg ataccaggct gtggaattgc accgctgcga cctcctccgt aaaaccgaag
    23761 aattgttgta ccgcgaaaca agcaagacca atattgatca atacagctcc atgatcctcg
    23821 aagaccttgg caaggtcctg atcacttccg aaagggaggg ctggaaacaa ctctatctgc
    23881 tcgaccttaa gaccaaggat ctggaatccc ttaccagagg gaatttttat gtgaaccagg
    23941 tgttgcacgt tgaccggaaa taccgccagg taatcttttc agcctccggg agagaaaccg
    24001 gacgcaatcc ctatcaccaa tatctctatg ccgttggcct ggatgaccgg cgcatccgat
    24061 tgctaacttc caatgaaggt aatcacgacc tctcggtttc cccggacggt aaatggtttg
    24121 tggacaatgt ctccaccctg gaccggccca cacgcagcct gcttagaagg gtaagcaccg
    24181 gaacctcttt actggaattg tcccgggccg acatcggcgg actggattcc atgggcttaa
    24241 aactgccgga gacttttaca gcaacaggca gagatggtca aacggtttta tatggggcaa
    24301 tttggaaacc cagccgtttt gatccggcta aaaaatatcc cgtgatcgac cagtcctata
    24361 ccggccccca tacctttatg tatccgcgaa actttatcag cggacttgcc cggtctaacc
    24421 aggccctggc cgaactgggt ttcatcgtca tcacggtgga cggcatgggc acggccaacc
    24481 gttcaaaggc ctttcacaac gtctcttaca aaaacatggg caaaaacctg gaggaccacc
    24541 gcctggccat cctccagctt ggcagccggt attcgtggat ggacacgacg cgggtgggta
    24601 ttttcgggca ctccgcggga ggctttgacg caggccatgc cgtattggaa taccccgatt
    24661 tttataaggt ggccgtggcg agcagcgccg atcatgattt ccgcatggaa aaggactggt
    24721 ggccggagat gtatatggga tggccggtgg attccaccta ccaccaggtg tccaacatta
    24781 cgatggcggg aaatctcaaa ggcagattgc tcatcaccca tggggggatt gacgaaaacg
    24841 tcaatccgtc ggctaccttc aaactggcgg aagccctgat ccgggccaac aaggagtttg
    24901 atatgcttat cctgcccagc cagcgccacg gttaccaggg catcttcaat gaatatttta
    24961 caaaaaagaa atggaattat tttgtggagc atttattggg ggcggtgccc ctttgggata
    25021 ttgatatgaa gggggactga ggccttccct tccgaaccac tgatgaatgc ttccgatcct
    25081 gtggttccat cgattggtca gatatttcaa actgcccacc ccaagctcaa caattattat
    25141 tctcccatta tttcatccct tacgggacga aattgccttt ctcaaatcat ctacccatct
    25201 taagtcccta acgggacaaa ttaccaggcg ctctccattt ctcttttttg aattcagccc
    25261 cacaaagctt gatggggtaa caaccaaaca ttgaagattt gacgttttgc actgccttca
    25321 tggttaaccc aatgagggat aaattaccag gcgcttctcc atttctcctt tttgaattca
    25381 gccccacaaa actgaagttg catatttggc cggatacggc gccctactca gggcatttga
    25441 tttgcaaact ttccttcccg ataaaatcct taataactca aaagcatagg aaatttgaat
    25501 ccgaggaata gcggatctct cccgtttaat ttcttaattc cttaattttt aatttcttta
    25561 atttctgccc aatatccgtt cccaaagtcc accaaagcct tcaaaccccg ccttacccca
    25621 gggtgcggat ttgaccagcc ggctgctgtc tgccgggtcc ttttgcaacg gataactgaa
    25681 agtaaaatct ccgggatcgg gcgcccagcc atgcatcgtt ccaaacctca gatcatggaa
    25741 ataaggcaag ccgtcgtcca aaatgctgat gcacgaataa cccaaggcaa atttctcaag
    25801 caaccgatat tccttgtttt gagccagggg agcagccagt tgtttttgag ccgggataaa
    25861 attaaaatgc accgggccgg gagtatcgaa ataggaagaa aatccctgga aataacctga
    25921 atcggaccgg gccacggtac cccacaggat attattcaaa gggccggggg aaagaaactg
    25981 gtcccggtat acgatgtttt gtttttgcag ttggtcccgg aacaaacctt caaaatgaga
    26041 tcggttgtaa aaagtccatg ccagatagat cgccgagagg atcaaggccc atctggccac
    26101 tttatttttg aagggcaagg aaaaccgttt aaacaggagc aggcagaaac caacaaacat
    26161 aggcagggta aaagccggat cgatgataaa aagggtggca taggtaaacc ggtgatcgca
    26221 gaagggttcc agcaagccgg ttccataagt cgtcagcgaa tcgataaata tatgggagaa
    26281 ggtccccagg gccagcaaac ccgcccattg ccaccagggg atcctgtcct ttctgtagag
    26341 ccgcgccagt aaagggccca caaccagggg aaacaaagcc gcgaaaaaaa tggaatgggt
    26401 gaatccccgg tgtatcaaca gggcttcgac cggatggaca aaaggcccgg caaaaatgtc
    26461 aaaatcagga acggtatcgg ccagggcccc ccaccagacg gcttttcgtc ccgccttttt
    26521 acccagggcg agatgtccca ggcagcctcc taaaaataca tgggtaaacg aatccacctt
    26581 tccctccctt aattcttaat tctttaattc tttaattcct taagcgccgc tacaaataca
    26641 tccaattcgg ccgttgaagt atagacgttg ggcgtgatcc tgcacccatg cacattggcg
    26701 taatcgatcg ctaccgtaaa gatcccgtac ttgtccagta aggtcttggc catgtccgcc
    26761 ggattcatac cctttatgcc cacgttggct atgccgcagg cccgggccgg gtcagccggg
    26821 gtattgagat caatatgcgg aaggtcgcgg accctggagg tccagtagtg ttgcaagtac
    26881 cttaggcgag cttctttgcg ggccgcgcca atcagttcat aaaaatcgat ggcatcgtgt
    26941 acggccagat cggtatgtac cggaggagtt cccgtatgat tgagtttgcg gatatcgtca
    27001 tcgggaaaac cggtatcccc gaacaactgc cacagtttgg cgattttttc cctcttgacg
    27061 tataaaagtc ccgctccaag aggtgcactg agccacttat gcaggctgct gccgtaataa
    27121 tccgcatgca gatccggaat attaaactca aaatgcccta cacaatgagc cccgtccacc
    27181 aggacatcca cgccgtgacg gtgggccata tccgcgattt tacgcaccgg caggatctgg
    27241 ccggtgatat tgatcatgtg gcagaccatg agcaggcggg tcctgggggt gatggcgctt
    27301 tcgtagaggg acacaatctc ctcatcatcg gcagggtgaa ggggcacgga aacccgatta
    27361 ttgaccaggc caaacctcct ggcctggtac ttgaacatgt ccagcatggc gccataatcc
    27421 tggatggcca tgacggcttc atccgccggc ctccagtcgt agcccgagat cacggtatca
    27481 agcgattcgg tggtgttgcg ggtgaggacc acttcttcat aggaggtccc aaccagggca
    27541 gctagttttt tccggatttc gatattgtct tcgaaacggt gattgcgcat ataccaggat
    27601 ccctgaaaat taacctcctt aaccctttca ataaaagcgt cctggatctg ccggggcatg
    27661 atgcagtaat atccgttttc cagattgatg taatccggtt taaggcggta accgctgcgg
    27721 acctttgccc agaaatcctc atcggcagcc agctccgccg gtggtcggtg ggctatacca
    27781 gctaccagct tttccatctg ggctagaaat ccggtactca acaggccggc tccgctgagc
    27841 tttttgataa aatcccgttt gttcataaat aaaaccaggc aagttaatcg cctacaatat
    27901 acaatgaaaa ccacccgggt tttaaactac tcattctccg aaaaaaggtc ataagctcct
    27961 tccaggtttt cctttaccgg cctgaccagg tccgggtcat tttctcctac ccggttgact
    28021 ctttcgctca cccggtaaat ctccgtggcc tcatccggat aggggatgat caattgcaag
    28081 gcttccgagg aggagaggct gggatcgatc cagagtttct cctgttcggg caggaggatg
    28141 gccggcatgc ggtcatgaat ggcacccacc tttttattgg aaggctgggt gaggatggta
    28201 aaggactcga cggcttcacc cgatggcgat ttccaggtct cccacaatcc ggccatggca
    28261 aaaagttttc ccccttccac cgtgatgcga taaggcactt ttttcttacc tacttttttc
    28321 cactcgtaaa atccgtcgaa aggcaccagg caccttcttt tttcaagggc gtttttaaag
    28381 gttttttctt gcacggtctc tacccgggcg ttgatcattt tataaccgat cttcggatcc
    28441 ttggcccaga aagggatcag gccccagcgc atcggccgaa aatgccgggg gtcctggttg
    28501 gtgatcaccg gcaggacatg agtgggtgcg acattaaaat tgggaagtgg attgtaccgt
    28561 tccaggtcct cgctgtaaaa gcttgcctgg aaccgttctt ccagttcttt ttcggttttg
    28621 gtgagggagg agcggccgca catggatgtg gaattgttga attgtcgaat cgttgaattg
    28681 taaggcggga ggtatacaaa gatctatctt taataggtaa aatgcgcatc aatcctcaat
    28741 cctcaatcct caatcctcaa ccctcaatcc tcatccctca atcctgtaag aatgctcgag
    28801 tgtgtgccca atttcagtga aggccgggat aaacaaatca tcgcccgcat ccgacagggt
    28861 atcgaatcgg tagccggggt gaaagtcctg cataccgata tgggttttga tgcccaccgc
    28921 accgtgatca ctttcgccgg aaaaccggaa gcttgtttgg aagcggcctg gcagtcggta
    28981 cggatcgcca ccggtcttat tgatatgcgt catcaaaaag gagcccatcc tcgcctgggg
    29041 gccgtcgatg tttttccttt tgtcccgttg cagggttcgg aaatgactga ggccattgac
    29101 tgcgcgcgga tactcgctga aagggtggga agggaactga ggatcccggt ctatttatac
    29161 ggagaagcgg ctctccgggc aaaccgtaaa aacctggctg aagtcagaaa aggggaatat
    29221 gagggtttga atgaaaaact taaactcgaa gagtggaagc cggattttgg cccaactgtt
    29281 tttaatgaaa aatcgggcgc cctgattacg ggagcccgaa aagttctggt agcctataat
    29341 ataaatctcg cgggcggaga cgccgccctg gcccaaaaaa tcgcgcggca gctccgggaa
    29401 aaaaacggtg gactgaaagc cgtgagggcg ataggttggt ggatgcctgc ttttaattgt
    29461 ccccaggtct cctgcaacct ggtcgatctt gacctcacgc cgctgcacct ggtcttcgat
    29521 acctgttgca ggctggcgga ggaaatgggt acccgggtta caggcagtga actcgttggc
    29581 ctggtccctg tgaaagttct gaccgatgcc gccgattttt acaaccaggg gaagacgatg
    29641 acggaaaagg aaaaattgga atttgtcaaa accaaattcg gcctgagtag tgtcaaaccc
    29701 tttgatccct caaaacaaat cattgagaaa gcgctgggcg gctggcaacc cggataaaat
    29761 cgcagggtct gcgattattc agaagtggac ccccagtatg atggacaata cattggacct
    29821 gcttttgtct tcaatcccgg aaggcgcatt ttctttatag atcctgctga gaccgtgttc
    29881 gtaggacaga cgggcagtca taaagagtac ccggaaactc atgccggcca gcaatccggc
    29941 gtgaatctca ttaaaatcct ccaccacgat ttgggggttt tcgtcaatgc caagcagaaa
    30001 attggcggct atgcccccgt gcattttcag atcggcaaat cggtttttat acacataaga
    30061 accgacctgg aaaggcagct taatggactt gaggtctgtg tattcaaaat aaggtttgct
    30121 gaggtcaagg tccacgggaa acagactggt cttcaggaag tacagcccgg gcatcaggaa
    30181 ataattcccg tcgtcgatca ctacatcgaa accaaaatga tatccctggt gcctcggatt
    30241 cctgcccggc cattgttcgt ctgtatagat ggtactgaac acgccggtat gaaaattgag
    30301 gccctggccg gtcagggggg ccgcaagcat ccacattaaa aagaaagcgg tagtttttat
    30361 gtaaatcatg gctgcagctg gttcgaagga ccgggtcatc ccggaaagac ccttactgat
    30421 tatttgccta aatttagccc ttaaattcat tgcatgtttc ccgacctctc ttatatattt
    30481 cattatcttt ttggtacccc ccccgatgga gctgcgtcca tcatcaaaac gtttggctta
    30541 ttcctcaccc tggcttttct ggccagtgcc tggatccttt acctcgaatt cgtgaggaaa
    30601 gaaaaacaag gactcttgca tcccaggccc atcatgatca atcctgccga cgcggtaagg
    30661 cctgcagata tccttatcaa tgccctgttt ggatttttta tcggatttaa ggtcctctac
    30721 gcataccatc acatggatga aatgaagact gatgccgtag ccctgctgct gtccctcaag
    30781 ggaaatatgg cggggggact gatcggcgcg ctcctgtttg gcctcttaaa ataccgggat
    30841 tatctcaaag taaaaaaaca ggattccaaa cccatggaag tcctgctgta tccgcaccaa
    30901 caagtgcccg atatcacgat cctggccgcc gtgtccggta tccttggagc aaagattttc
    30961 gccttatttg aaggcgaaca atcctggagt tcatttgtcc gggacccggt caaagctttt
    31021 ctttccggca gcggcctggc tatttacgga ggcttgatcg tggcctttat cacagtcttc
    31081 tattatgtaa agaaaaaggg gatcaaaccc attcaggtca tggatgccgt ggcgccggct
    31141 ttgatcatcg gatatgcggt gggccggctc ggttgccagt tttcagggga cggcgactgg
    31201 gggatcgtaa acctcaatcc caaaccttcc tggtttatct ttccggacag ttggtgggcc
    31261 tacacctacc cgcacaatat cctcaatgaa ggggtgccga tcgcggactg cagctaccgt
    31321 cattgcatgc aactggcgca gcccgtatat cctacaccgc tctgggaaac ctttttttcc
    31381 tttgtcattt tcgggatcct gtggtttttg cgtacccgga tccgcatcgc cggcatcctg
    31441 ttttttattt attgcttcct gaacggagtt gaacggttct ttattgaaaa aataagggtc
    31501 aacgaaaaga tccatatcgg ttccctgtcg ctgacccagg cagaaatcat cgctatcctg
    31561 ctgatgatca ccggacttgt aggcagtttc ctgttgtgga aaaggcataa accgtctgtc
    31621 gcctcctgat ctcacaccct tcgcagtgac cgctgtcgac gaataccggg cctgggctga
    31681 actaaaagga aatgcattgc ccctttttct caggccctgg tggctggatg ccgtgtgcgg
    31741 agacagccgg tggaacgtgg cactggttaa aaaccatggc ggagaaccca ttgctgccct
    31801 gccttttttt caattttaca aaagaggaat tcacgtcatc gccctccctt ttctgacccc
    31861 ctacatggga atccatatcg attacccgga gggacaaaaa ctgacttcaa ggtatgacta
    31921 cgaaaaacag gtactcgatg gcttgatcga ccaattgccc gatactcctt acctgcaaca
    31981 gaagttttat ccttccctga cctatggact tccttttttc tggaaaggat tccggctgga
    32041 ttggagatat acctaccatc tttcactgca ggaagaggaa aaaacgatct ggaaccagat
    32101 ggaaggggcc gtgcgtaccc aattgaaaaa aacctccggc caggtggaaa tcgtggaaag
    32161 cacggaccct gccttaatgc ataagctggt gaccatgacc ttccggcgcc agcaaaaaaa
    32221 aatgccctat tcccgggacc agtttcaacg gctttacgaa tcggtgcaaa atcagggagc
    32281 tggtaaaatc ttactggccg tcctcccccc gagccaggaa ccggtggccg ggatattcat
    32341 cgtgcatgac cgggatacgg tttacaacct ggtgttggga cgcaaccacc tgcttgaccc
    32401 cggcggaagc atacacagcc tcttgtggaa agccatccgg gatcatatcg gccggtaccg
    32461 catttttgat tttgaaggca gcatgctgga agggcctgaa cacgtattcc gttcttttgg
    32521 gagcctccgt atccccgtat tgcaaattac ccgctaccgg aacaggctgt ggaaagcggc
    32581 cctggccctg gccggtaaat aggctcccgg tttgatcatg acaaaatggg cctaaatctt
    32641 tgccggtcac cggattgaat gcccggacca ttccacttac ctttgtcccc ctgcctgttt
    32701 acccgatgga tattctgatt aaaaacaagg tactggctac tatggccggg ctggtggcgc
    32761 ataaccgtgc cggcctcctg gaagccaatc aacaagacct ggatgccggg aacctggatg
    32821 accttacctt atacgaccgt ttaaaaatcg acgaccggaa aattggggcg atgacgcagt
    32881 ccctcgagga aaccctggcc ctcccggacc cggtgggtat tgaaaaatat cattaccatc
    32941 atccccaggg aatggagatt tacaaccgca gcgccccttt cggtaccatc ttgatcattt
    33001 atgaatcccg gccggacgtc accgtagagg cggcagcgct tgcttttaag gccggaaata
    33061 aaatcttgct caaaggcgga aaagaagcga gaaactcaaa ccgcttcctg gtaagtctat
    33121 ggcacctggc cctggccgaa tgcgggctgg atccggacca tatcctctac ctggattata
    33181 cccgcgaaca aacctttgat ttgctcaaat cctcagcagg caagattgac ctggtggtac
    33241 cccggggtgg cgatcaactt attgagatgg tcaaaaccca tgcctcggct ccggtcctgg
    33301 tcagcggcag gggtaacaat tttatttatg tacacgataa agcggatccc gccatggcgc
    33361 tggagatcat cctcaattca aaactgcaga aaatcagtgt ctgcaatgcc accgacaagg
    33421 ttttgattga ccgtcagctt ccgggactgg aagctttttc taaacaactc gccgctgcct
    33481 tggtctatga agaagtttcc ctcctggcag accagagcct gtccggcctt ggaagcctgt
    33541 atcctgttca aatccccgac caggaaaaca tctggtatga agaattcctg gcacgtagga
    33601 tcctgcttgg gaccgtaagc ggcctcgacg aagccgttca aaaaatcaac aaatattccg
    33661 gagggcatac ggcagtgatc atcacagcag accaggaagc cgctgccggt tttatggaag
    33721 cggtagatgc cgcttctgtg atccataacg cctcctcgcg cttcacggac ggaggccagt
    33781 ttggactggg ggctgaactg gcgatcagca cggacaagtt gcaccaccgg ggccccctgg
    33841 ggcttaacca gctggtgacc aataaatggt atgtatatgg caatggccag atccgaaaat
    33901 aagcttcagc atccatggta aacaaaagga aacggattgt actcaaactg ggcagcacta
    33961 ccctgacccg gggcactaaa ctgatttcca ggggtaaact ggaggacatc gcccgccaaa
    34021 tcaaccagct taaaaaagaa aatgagatcg tgatcgtcac ttccggggcg atcgcggcgg
    34081 ccaaacatta tatcgaactg caccggggca atccggtggc cgtcaaacaa gcccttgcag
    34141 ccatcggcca accggtgctc atgcgttttt accaggaagt gttcaacgat tatgatttga
    34201 agatcggtca gtgcctgctg acctaccggg attttgagaa tgacctgtcc caatccaata
    34261 ccctcgccac catcaacact ttgctggaca acgggtatat cccggtgatc aatgaaaacg
    34321 acaccatcgc caccgacgag atcaaattcg gggacaacga taaactgggt gccctgaccg
    34381 cggtattgct caaagccgac ctgctcatcc tggcttcaga catcgacggg ctttatgatg
    34441 ccgacccccg gcaggacccc aaggccaggc tgatccatac cgtgaaagac ctctctgaaa
    34501 tagagcattt gcaaaacgga ggcctctctt ccctggggac cggtgggatt aaatccaaaa
    34561 ttgaagctgc ccgaatatgc cgggctcacg gaatagaaat gtggattgta aatgggggta
    34621 aggacaattt tgccgtgaat gccctggaag gtaaaataac atataccgga ttccccgtat
    34681 aatcggtcta atcctcaaac agttccagct gcaccggctg attccagtca aaactcttcc
    34741 ggtgatgctc gcattccccg tgaagcaata aagccgactt gtgttcaggg gtcgcataac
    34801 ctttattgtt gacccagcca taatgaggat attgctcgtg cagccggcgc atatattcgt
    34861 cgcggtgggt tttggccagg atcgaagctg ccgcaatgga gaggtagagg gcatctccat
    34921 ccaccaggca ctgatgcctg atttcgccat aggctttgaa tcggtttcca tccaccagga
    34981 tcaggtcagg ggggatgctc aactgggcaa gggcgaggtg catggccctt accgaagccc
    35041 aaagaatatt gagctcgtcg atttctgccg gggtacagga ggccactgcc cattgaaggg
    35101 ctttcttttc aatgattgac cggagccggt ccctttcttc agcctgcaat tttttggaat
    35161 ccttaattcc cggattccgg aatcccgggg ggaggatcac cgcagcggcg tagaccggcc
    35221 cggccaggca tccccggccc gcttcatcca ctccggcctc gatcaaccct ttctgaaaaa
    35281 atgacttcaa gtccagctgt ttgcgccaag gtatcattaa tctttgtttt atcgggggga
    35341 atggggctaa atttgcaccc tgtttacatg ttaaaaagac cgccattcct gctttttgcc
    35401 gatgccaaag gcctcaccta tgaggacaaa agcctttatg ccatgggacg gtcggcctgg
    35461 gatgcggggg aagtgcccct ggaagaatgg attgaattgc cggaaggcgg acaattgtac
    35521 accctccccg accgccaggg cctgggtata gatattgctt ccggcgaggt gaaagtttgt
    35581 gaaaaaggaa gggcggttgc cgcttttgta ccgcctgctt atacggttct aagattgtcc
    35641 ccctatctca ccctgcctga ggccagcccc cttccccttt attgttacag cgcggtagga
    35701 tggtatggag gcaaaatata cgtagccgct acccggattg aaaaggacat ccgccaggag
    35761 gccgcgggat atgatgaaaa aaagatatcc aggggcattg cccaactcac ttccgcttat
    35821 cccaacaaca ggttggtcag gcaccttgcc gaaaactgcg ccatgcgtta ttgctgcccg
    35881 gctgcccgga attatttcct cggcaggtgg gaatgcccgg taccggtttc cccggcctgt
    35941 aatgccaact gcctcggatg catttcctat atcccggaaa gcgaggtctt ccaggcttcc
    36001 catgaccggc tggccttcaa accgactgcc gcagagattg tggaatatac cgtaccccat
    36061 cttgaaaccg ctccttaccc catcgttagt ttcggacagg ggtgtgaagg cgaacccctg
    36121 ctgatgtggg aaaccatccg tgaatccatt gtacaaatcc ggaaatttac ttccaaggga
    36181 tctatcaaca tcaataccaa cggcagcaaa ccggctgcgg tgagggcttt gtgcgaagcc
    36241 ggattgaatt ccatacgcgt atccatgaat tccgcccggg aaaccctgta cacccggtat
    36301 tacctcccta acaattattc tttcagggat tgcctggaaa gcctccggat cgtgagggaa
    36361 tacggcggat ggtcctccat caattatttt gtattccccg gcgtcaccga taccccggag
    36421 gaataccagg ccctgtgtga cctgatcgat tccaccggcc tttccatgat ccagtggaga
    36481 aactttaata tcgatccgga tttttatctc cataagttga atatcccgcc ggacgagcct
    36541 ttcctgggca tcctgaacat gatgaaaaaa ctccgaaaaa ggtatcccga tttgaaattc
    36601 gggtatttca atccgccccg ggagcggatg ttcaattacg agacggactt tgcccattaa
    36661 agggtcatcc cgctgaacga acttgatcca ctcccttccg tctcatgggg atccggtcgc
    36721 cctccatgaa aaatcaagcc gggcaaaaga ttatcgttta actttgaaat cctctttttt
    36781 ccgaacatga taaaattctg gtgttttgtt tttgcccttg gattaggggt cacggcacgg
    36841 gcccagaaag tgaccgtttc gcaggaaatc acactccgta acgacctttc ttatgatctc
    36901 gtgggaaaga tcgacagcca ttttattctt ttccgtgacc gaggcaccaa atacgaagcc
    36961 agcatatatg accaggagct taaattaaaa acggaaaagg aaatcattct ggcggataaa
    37021 acctgtcagc tcctggcgtc cctggccaca ccccaatcct tttttatttt ttatggtttc
    37081 cggcacaggg gagattatta catccaggcg gaccagctga acggggagtg caccatcatc
    37141 tcttcggaca ccgtcaaaat attcgaaaac gtgatcctca atcccaatat ccggtttgct
    37201 agatcggagg atcgcacaaa aatcattttc ttcaccgatg aaaaggatca tgaaatgcag
    37261 gtggcgcttt atgatctgac ggccgggaga acggtttacg accgcaaaat ctatttcaag
    37321 gaaatttttc tcaaggagga tttccggtcc ctgctggtat ccaatgccgg cgaaatgttt
    37381 ctcatactcg aaaaaaacaa ttaccgctcc cgcaaagaga agcacgtatt tgaagtccat
    37441 tgtttttatc ccacggcaga agcggcccgt atgtttgtca tcccggtttt ccgttttgtc
    37501 agttttcaca gcaagtttgc ctacgacaat gtcaataaac aattggtggg agggggcatt
    37561 tattcgcaga aaaacctcaa ccgggcggaa ggggtctatt acctcaaaca ttccctgcaa
    37621 atcggagagg aagaacgttt gacgacttac gcttttgaat cgcgttttga tgaagaaatg
    37681 accaggaggt caagggacaa aaacgaattc agccagttgg aagtcagcga gatcgccctg
    37741 cgccaggacg gtggcgccct gctcattatg gaaattaaaa aagaattcga acggcgttcg
    37801 gtatacggca gcggccggcc gctgaacaac ccgggaggtt tccgcaatta catgctggtg
    37861 gactactatt atgaagatat ggccttgttt tcaatcaatg ccgatggcag cctgatgtgg
    37921 tccgatatcc tgcataaaaa acagtactcg cacgacgatg acggggtctt ttcttccttt
    37981 ttccttttca aaaatccatc ccggctccga ctggtttata acgacgagat cagttccgaa
    38041 aacacagtga gcgaatacgt ggtccaggcc gatggtcagt accagagaaa gagcgttttg
    38101 agtacggaat acgtgcgcct ccagttgcgg ttcaaggatg cggtgcaggt atcccactcc
    38161 tcatttattg tcccttccca aaggtcgatg cggctcaatc tggtcaagat cgaatactta
    38221 taggattttg ccttaagcgg gactttccta ccaacgacct aatgggtcag ctatctagca
    38281 taataaatta ataatcaaag ttttatgttt ttaggggtct ttgtggcaaa gcattttttt
    38341 acttcccgga cacccccacg tcccacatcc tggatgcggg gaaaaatcat gcattatttt
    38401 agaaataaca tttctttcag ccattttttg ccgaaaacaa aacctccttc aggcagatac
    38461 gcgtaaaaaa tctaatttta cagccgtttt taaaccaaaa ttcattagaa atggcaattt
    38521 cccgatttaa agccctggac ctggctcaaa accgtcccat gcctgctgtc aaacttccgg
    38581 accagaaaat caccgaatat tacggatcca acacgttcaa cgacgacgtg atgaaatccc
    38641 tgctgagccc ggacgcttat aagaaaatca acgaagccat cctcaccggt tccaagattg
    38701 accgcgaatc cgcggacgaa gtggccgcgg ccatgaaatc ctgggcggtc tcaaaaggag
    38761 ctacccatta tacccactgg tttcagccgc tcaccggatc caccgccgaa aaacacgacg
    38821 cttttttcga tatcgacttc aaaaccggta aggcgctgga aaaattcaaa ggctccgccc
    38881 tcgtacaaca ggaacccgat gcttcctcct tcccttcagg agggatccgc tccacttttg
    38941 aagccagggg ttataccgcc tgggaccctt cttctccggc attcattatg gaaaacctgg
    39001 ggggcaccgc caccctttgt attccttcgg ttttcgtttc ctataccggc gaagcccttg
    39061 attataaaac ccccctgctc aaagccattg ccctggtcga caaagccgcc acggccgtga
    39121 tgaaagaatt tttcgaccgc gacgtcaaaa gagtgatcgc aaccctgggc cccgaacagg
    39181 aatattttgt gatcgatgaa gccctgttta atgcccggcc agacctgatg atgtccggac
    39241 ggaccgtttt cggccatgct ccggccaaag gccaacagct ggaagaccat tattttggtt
    39301 ccatcccgcc gagaatcaac gccttcatgg tcgactttga aattgaagcc caaaaactgg
    39361 gtatgcccgt gcgcacccgc cacaatgagg tcgccccggc ccaatttgaa gtcgccccga
    39421 cctttgaaga actcaacctg gcctgcgacc acaaccaatt gctcatggac ctgatgaata
    39481 aggtcgccgc ccgccataaa ctgaaagtgc ttttccatga aaaacccttc gctggcatca
    39541 acggctccgg aaaacacaac aactggtccc tgggcaccga caccggcatc aatcttctgg
    39601 gcccaagttc caaaccaaaa gaaaatctcc gtttcctcgc cttttttgtc aacgtgatca
    39661 aggccgttta cgataattcc gatttgctga gggcctccat cgcttccccg ggcaatgagc
    39721 accggctggg cgccaacgaa gcacccccgg caatcatgtc tgtctttgtg ggaaatacca
    39781 tgaaccgcgt gctcactgaa tttgaaaaga acaaggagat taccctggaa aaaggagaca
    39841 atgtctatct aaaactgggt gtcaataaga ttccatccct tttgctggac aataccgacc
    39901 gtaaccggac ctcgcccttt gcctttaccg gtaataaatt tgagttccgc gccgtggggt
    39961 ccagcgcaaa ctgcgcccag gccatgaccg tactcaatac catggtggcc gaccagctct
    40021 ttaagtttaa agcggatgtg gacaaactga tcgaaaaagg ccagaaaaaa gaactggcta
    40081 tttcagaagt ccttcagggt tacatcaaag cctccagaaa aatcattttt gaaggaaata
    40141 attattccga agaatgggtt aaggaagctg ccaaacgcgg tctgggtaat atcaaagaca
    40201 ccccttccgc ccttgaggtc tacctgagga aacccagtgt ggatttgttt gaacgccatg
    40261 gcatctatac cgccaaggaa ttgcacgccc gcgtggagat cgaatgggaa aattacatca
    40321 agaaagtgca gatcgaaagc cgggtgctgg gcgaccttgc catcaaccac atcgtttcca
    40381 cggcgctgaa ataccagacg cagttggtgg acaacgtgcg cggtcagaaa gagatcggca
    40441 ttcccgcaaa ggtttaccaa cccaccgtcg aaacgatcaa aaagattagc gaatacatcg
    40501 gcaagatcca aactttggtc gtagacatga cggaggccag gaaaaaagcg aatgccattg
    40561 aagatgcaca caaaagggcg ctggcttata accaaaaggt caaatccttc tttgatacca
    40621 tccgttacca gatcgacaaa ctcgagctga tcgtggatga cgatgaatgg cctttgccca
    40681 agtaccgtga attgctgatg gtgcgataga cagcaatagt caatagtcaa tagtcaatag
    40741 tcaatagtca atagtcaatg gtcaatggct ggtgattgga aacaaagact ttaaagctaa
    40801 agcaccttag gattggaaag ttctaaggtg cttttatttt gaagataacg ggggcgctcc
    40861 cgtgatccct acccctttcc tgatcagaac ctcccggaag ccgggaggct taagtgtttg
    40921 ctgtggcggc agtattacag atctccgttc gcttcccgat aaatcaccgc caggaccacc
    40981 tgcccggtgg ctttcagggc ttcccgatcg atgttttcaa tcttgtcctc cagggtgtgc
    41041 caatgagcaa caaattcatt attggtcggt cggttgatga tatcaatcat ggggatattg
    41101 gtcatcctgt ttatgaaata atgatcgtca atgatcgcgg gggagacctc gtttacaaaa
    41161 taatttccat aacccatgcg ctgcgccagc gtccatactt tatccacgaa ggcggaagca
    41221 tattgtacgg aagtgccttc tttggtaaaa cgcggggact ttcctcccac catatccagc
    41281 aggatcccgt atttagcctg ataccctgcc gtatgtttgt gcaaagccca gtgctgcgcc
    41341 ccctggcacc aggattcggg atccgaccct tcccctccct ggtcttccgc gtcaaaaaac
    41401 aggaggtcaa tacccaggtt ggggatggga ttggcctgaa gctgcctggc gatctccagc
    41461 aatacgccta ctccgctgcc cccgtcgtcc gcaccgggca aaggcgtgtt tttatttttc
    41521 atctcgggat cctgatcggc gatgaaccgg gtgtcccaat gggctgccag gatgatccgg
    41581 ggcctgactt tttcattaaa ggaagcaata atattccggg cctggaaaga tatccctgaa
    41641 aaggtcttgc ctgtaaaagg ctgctcgatc acccgggcac cgcaggcttt cagcctgccg
    41701 accatccagt ccccgcaacg tttatgggcc tcggtacccg gcactcttgg cccgaagtcc
    41761 acctgggttt gtatataagc aaaagcggaa tccttgtcaa agaccggtac cttgagtttg
    41821 acgggcaggg cggccggggt ggtgatgttc gcttcgttct tacaaccgaa atagaacagg
    41881 gtcgaggtgg ccagcaccaa gctgaatatc aagcgccggc caaaggcata ttgtctcatg
    41941 ggatgtgcaa taattattcg tgagtccagg tagttccttc cttgctgtct ttgagtacga
    42001 ttttaatttt acccaaccgg tcgcggatct ggtccgataa ggcaaagtcc ttcctggccc
    42061 tggcttgctg cctaagatca atcaacagtt tcatgatttc atccatttgt ttcccctgac
    42121 cattggacat ttcttcgtct ttcaggccga ggatatcgta aacccagagt gcggtctctt
    42181 ttttcaggcg gtctagggtc tgcgaactga tttgctgaat gtcgagatgt ccgtctttaa
    42241 aggagttgat ccgggtgacc aggtcaaata aaaccgccag caccttgggg ctgttgaaat
    42301 cgtcgttcat ctcggtgtat aaagaatcca attcggcccg gatttgctca tccagggacg
    42361 cttgcagccg ggtcccctcc gtgcccaggt ttctgagtga tcgtacggcc tccatcagcc
    42421 ttttatatcc cttttccgct gcctgcaatg cctcgtcggt caggtccagg gtgctgcgat
    42481 aatgggtttg aagcataaaa aaccggacga ccatgggtgc ataggacttg gaaatatgag
    42541 ggctctggcc gctgaacaac tgagcgggag aaatggtatt gccatcgctt ttactcattt
    42601 ttttaccgtt cagcaggagc atgttggcat ggagccagta tttggcaggg ctgtgaccgc
    42661 aacatcctat gttctgggca atttcatttt catggtgagg aaatttaagg tcataacctc
    42721 ctccgtggat atcaaagcgg ctacccaggt atttggtgct catcgcactg cattccaggt
    42781 gccagccggg gaaaccctcc gaccagggcg aaggccagtg catcagatgc ctttcatccg
    42841 ccttgatcca gatggcaaaa tccgccggat ggtccttctc ctcctgtttt ttaagggccc
    42901 gcgattcatt ttgcagctca tccatgacct taccggaaag tttgccgtaa acatcatggg
    42961 tttgaatcag tttttgagtg ttgaaataca ccgatccgtt gcgttcgtat gcatagccat
    43021 tggccaggat cgtttttacc atttcaattt gttcgacgat atggccggta gcgcggggtt
    43081 cgatgccggg cggaagcgta ttgaacactt ccatcatttc atggaatccg ttcatatatc
    43141 tctgcacgat ttccatgggt tccagttgtt ccagcctggc ttttttggat actttgtctt
    43201 ctccttcatc ggcatcgcct tccaggtggc cgacgtcggt gatgtttcgt acatacctga
    43261 cctggaatcc aagttgccgc agataacggt agatgaggtc aaaactgaca aaggtcctgc
    43321 aattgcccag atggacgtcg ctgtataccg taggtccgca gacgtaaagg cctacccggc
    43381 cttcgtgcaa gggttcaaaa cggacgtatt cccggctcag gctgtcgtaa ataaaaagct
    43441 cctgattcat cggggcgaag gtacggattt aaaggttcgg atgagatggg cttggggagg
    43501 ggaggagggg gaattattcc tttttcccgg cttgtgattt tcctttgggt ttcatgtgtt
    43561 taaacatttc cacctgttta agccgctgtt cggcctgctt aagggtgagg ttatcctttg
    43621 acagcggttt ccccttttcg gaaagcacct ggtatccgga gctggtttta acgatcatgg
    43681 catttacagt ttccctttat ccattctttc catttccctg cgattgtctt ttgccctaat
    43741 ggtctcccgc ttgtcgtagg cttttttgcc ttctcccagc acaatttcca ttttgatcag
    43801 gcctctttcg gacaaataca tgcggtaagg cacgatcgtc atgccttttt catccacccg
    43861 gtgcctcagt tttttaagtt catttttttt aagaagcagt tttctttccc tcagggggtc
    43921 gtgtaccgac cagcctgcgg cctgtttaaa cggggctata tgaaaatttc gtatgtataa
    43981 ttcaccatcc ctgaagtaac aataggcatc attcaggttg gcattgcctt cccggatgga
    44041 ctttacctcg gctccgtcca gcacgatccc cgcttcaaag gattgccgga aaaagtattc
    44101 gtgctgcgct tttctgtttt ttatctccat ttaagctctt aatcgttgcg gcgaaagtaa
    44161 aaaaataaca attttgtaca ggattccgtt tctataatga tgtttttaac caggatcgac
    44221 aggatacgca ttcgtgtctt tattcttttg gctgtcagcg tctgcctcta tcccgcttgc
    44281 cggcatcacg agtccacccc cgacagtcag accagggcgg tcaccctgaa acaaaaagat
    44341 ctgatcctgc ccgaccggta caatgtagcc tttctggtga tgaacggggt gtacaatacg
    44401 gaactcaccg ccccttacga cgtttttcaa cacacccagt accgggaaaa catcaaagcc
    44461 atgaatgtgt tcacggtagc gaacacccat gatctcatcc gtactttcga aggactctat
    44521 atcaaaccgg attttgatta tacccgcgac aaactgccca agatcgatgt cctggtcatt
    44581 cccagcgcgg aacaccatac cgactccgat ctcaacgaca ccgtcatgat ccgctgggtc
    44641 cggaagaccg gggagaatgc gatgtttgtc acctcccatt gcgatggggc ttttttgctg
    44701 gccaaggcgg gtttgctgga tggggtctcc tgcacgacgt ttccggccaa cgtcaaaacg
    44761 tttaaagaca tgtttcccca ggttcgcgtt tatgaaaatg tttattttgt tcacgacaaa
    44821 aatgtgatta cctcctccgg gggagcccgg tcttttgaat cggcgctgta tcttgttcaa
    44881 ttgctttatg gaaaagttgt ggccgacaaa attgccaagg gcctggtcat cgactgggat
    44941 ctcaatacgg tgaaaaaagt ggtcatccat cagggcaacg gttattccac cttatgagca
    45001 ggctgatctt gctggttttt ttattcggac cggccggaat gcttaccggc caaaatccct
    45061 ttgacataaa aaaaccggat gcttcaaaaa tcaaaccggc agtcaaaaaa gaagaagtaa
    45121 aaaaaaccga agccatcgat ccgggtgcgg ccacccagaa aaagactacg cacgattcct
    45181 tacccgttgc agttcagacc aggcctgcgg agatcgttcc ggccccggtt caaaaaacgg
    45241 gtaggacttc tgccaatata tttgatatca aatcgggagg gaatcccaat gaagtcatcc
    45301 tcaacaagcc aagtcaaagc ccggaaggaa cccggcctcc ctttccatcc tcccgggaca
    45361 gatccgctga aacggccgga gtcgacacct ccacaggaag tatcccccca tcgtctgaag
    45421 ccaccgggga tcccaacggc cggcctggag gagtcgatct ggatgtcctt caaaaaaagc
    45481 tgaaaacctt tggaaacaat accaccgatg cccgcggagc gatcagtaag aacgcctggt
    45541 ttttgatcta tgtctttctg ttcctgctgg ctgccatcgc catcaatttt aaccggtctt
    45601 atccggcctc cctgttcaag gctacctaca atcagaatca actccggatg ttgtttaagg
    45661 acgccttcaa aggcaatcac atgatggtct tcggcatcct gtattttctg tttgtggtca
    45721 atgccgggat ttttatttac cagactttcc agattttccg gattgctccg ctttccctcc
    45781 tggtcgccct ggggatcgtc ttgggcatct acctggtgcg ccacctggtt ttgtatctga
    45841 tgggaaaaat ttttccaatg gaaaaagaaa ccatgttttt caattacacc attggaatcc
    45901 acaacctcac cgcagggctc ggattgatgc tcatcaacat cctgctgtcc tttgtcgatc
    45961 cggcttcagg aaagaccctg atccttgttg gaatatgcct gatcggtctc ctttacatca
    46021 tgagacaggt gaggggtctg ctgaacaacc tcccgatcat agtttcaaga aaattgcatt
    46081 ttattattta cctttgtgcc gttgaaatcg caccctggat gttgttggtg aggtattttt
    46141 ccctctaaat atccgcctcc gcaaaccggg tactatttaa aaaccaggta aacttgattt
    46201 cttgaatgag cagcacagcc aagacttctc cggaaaagtt caaacgcatc aaaaccatac
    46261 tggtttctca acctaaaccc gagcattctc cctatgcaaa tattgagaaa aaatacaatc
    46321 tgaaaatcga ttttattccc ttcaaccaga tcagcccggt caccgaaaaa gaattcagga
    46381 aaagcaggat ccgtccggat gaattcacgg ccgtggtctt caccagtaaa acagccatag
    46441 aacattattt caggatctgt gaagaaatgc ggattaaagt caatccggac atgaaatatt
    46501 tctgcctcac ggaagccatc ggcaattacc tgcagaaatt tatcctgtac cgtaaacgca
    46561 aggtatttgt gggcaccaaa agtattgaag acctcagcgc ctattttaat aaacacaaaa
    46621 acgaaaaatt cctgctcccc tccagcgacc agggtgcaaa agaagtactg gattacctcc
    46681 tgggtaaagg attaaaagtc acggaagcca tcatgtaccg gacggtcagt tgcaatcttt
    46741 cccatctcaa agacatcaaa tacgacgtcc tggttttttt cagcccgctt tgcctgaaat
    46801 cgttatatga aaacttccct gatttcaaac aggaagaaac ccgcatcgcg gccttcggaa
    46861 cccaaacctc caaagcagtg gaagatctgg gtctgaaact gaacatcaaa gcgcccgccc
    46921 ccgacgttcc atcgatgagc atggctttgg aaaaatacct gaccgtctcc aacaaataaa
    46981 atgcctgagc tggccggccg actgatttca accggtttaa taagcctttc catcccggtt
    47041 ttctttttat gcaagaccgc cccagattcc ccccaggcac ccctccctct gaaagccgcc
    47101 cggccgtcgg tgagctcccc ggctacagat accctgaatt atgtgatggg caaatttgat
    47161 ccgggcagca atgaacggtt tgcccccgtt gaacaaaaat acgcaagccg tgaagggatg
    47221 ttcctcagga aagaaaccct ggaagcattt gtaaaaatgg caaaggccgc agagacggac
    47281 ggggtagaac tcaaaattct atcggccacc cgcaacttta ccagtcagaa atcaatctgg
    47341 gaagataaat ggaccggcaa acgcctggtg gaagacggcc agaatcttgc cctcaccacg
    47401 ccagatccgg taaaacgggc cttaaaaatc ctggaatact cctccatgcc cggctcttcc
    47461 cgccatcatt ggggaaccga tatggacctc aacgcactgg acaattcctt ttttgcaaaa
    47521 ggcgaaggaa aaaaaatata cgattggctg gtcgcccacg ccgggaaata cggcttttgc
    47581 caaccctata ccgccggtcg tccacacgga taccatgagg aaaaatggca ttggtcttat
    47641 atgcccctgg ccaggccgtt aaccggtttt tgcgaacgcc atttaaaaaa tgaaatgatc
    47701 agcggttttc tgggtgctga gacggcaaaa caaatcaata tcttgcagca ctatatcctg
    47761 ggtataaacc cggattgcag atgacacggc ggattattaa agttcctttg ggtacaggat
    47821 ttcattcccc tttttccgga tccaatgcat cagggagagc aactgttcat ctgtaagatc
    47881 gggataggcg ggcatcccca tatttaaccg ttcacccttc tgaaccacca gcctgaaaag
    47941 gtccaggttt aaagcaatag ccgaggcttt taggtccggg gccatacctg ccgccagaag
    48001 tcccggacca tgacagccgc tgcattttcc atacaaggat tgtcctgctt ttgccaaaga
    48061 atcattcacc tcaaaatccg gcgcatccag aggaacggga aaatagggag gaggcaaggg
    48121 gggcaaagcc ttcttgcctt ccagtgaaaa tacgatcagg cggcgcatat gcttgccgta
    48181 ttcccaaccc agcgccttgt ttccaacgcc cgcaaaggcg ccaccccaac ctaccagcag
    48241 ggccaggtat tgtcgtccct tcaacttgta ggtaatcggt ggagctgaaa tacccaggcc
    48301 cgcatcaaaa gtccaaagga tctcaccggt ggaggcatga taagccagga attttccatc
    48361 ggccctgccc tggaaaacca atttacccgc cgtggtgagg gtccccgcat tccatagttc
    48421 ctgctggggt acggaccagg ccaatttctg ggtcaccgga tcccaagcct ggagagaggc
    48481 cggataatca cggggcaatt tctcaggcgt tgtaagtccc actccgatcc cgcccttaaa
    48541 gtcaacggcc tgccaggaag ccaggtctat gccctggtcg gaataaacat tgccgtcgtg
    48601 tatggagggg atataggcca gcccggtgat ggggttgaag gacatggcat gccagctatg
    48661 ggctccatga ggtcccggag tcacatagcc gggaccctgt acataccgcg ctgccgctat
    48721 ttcaaccggg cgaccggtgg ataaatcgat ttctgaagcc caggaggttt ccacaaacgg
    48781 cttggctgaa atcagtttcc cggtttcccg gttgattaca taaaaaaatc catttttcgg
    48841 cgcatgcagg atcaccttag tttttattcc ttccagcatc aggtcagcca ggacgatatc
    48901 catgttggag ttataatccc aactctcgcc aggcgtggtc tgataatgcc accggtactc
    48961 cccggtatcg ggatcaagcg ccaaaatgga agaaaggaac aaattgtccc cgcccccggg
    49021 actcctgatc ttccggttcc agggagagcc atttcctgtc ccgatataca acaaatcaag
    49081 ttcgggatca taggtgaatc catgccaggc attgccgcct ccgccatgtt tccaccattc
    49141 gccggtccag gtccctgctg ccattctcat ggcttcattt tcaaatccgt cggccggatt
    49201 tcccgggacc atataaaatc tccaggcttg ttgcccggtt tccgcatcat aggccgtgac
    49261 atatcccctg gagggccctt gttcggtgcc tccgttcccg atcaccacct tgcctttaaa
    49321 ggccttgggc gccccggtaa taaaccaggg tttgttcaga tcaaaggtcc tggtactcca
    49381 aatcaatttg cctgacccgg catcgagggc ttgcaagcgt ccatcccagg tagccaggaa
    49441 tatcttaccc cggtaatacg acaaacccct gctatggctc cagccgacct tgcggtgatt
    49501 gccaatttcc tttgcgattt ccggatcgta agtccagagc gctttactat tggtggcatc
    49561 gaccgcgcgg acgacattgc cggtgcctgt aaaatacagg ataccatcaa tgaccagggg
    49621 ggtcgaaacc agggcgtggt cgcggggcag gtctaaaaac cagtctacct gaagacggga
    49681 gacatttgaa gtatcaatat ccgtcagcgg gctgaaacgt ctctctgaat gggtccttcc
    49741 ataagccaac cagtctgaag tgtttgattc atcggccagg tcttgttccc ggtcggttcc
    49801 aaccggttta ttacaggagg gaaaccagcc ggtcaataga atgagggtca ggacacaata
    49861 ttgaacggaa aatttataca tcgggagcgt ggattggtta acggaacaag tttaccgggc
    49921 gagtttttta atgtcgctga cttccaggac ctttttatca taccctttca ggacagcatg
    49981 gatcatggcc atcccgattt cttttaaagt acagatccgg ttgggcgcca gccagcggat
    50041 gatcggaacc agccagaaaa ggtatttata ggatcgaagg gtgtttttaa ggccggcccc
    50101 cggttcaata aaacccggcc ggaatagaaa tactttttta aagggaagtt tctggaggtc
    50161 attttccgtc ctgcctttta ccctcgccca catcattcgg cctttttctg tgctgtccgt
    50221 tccggcgccg gatacataac aaaaaaccat gcccggattc agggcaacca tactggatgc
    50281 caggtaaagg gtaaggctat gcgtcaatcg tgtgtattcg gtttcggaaa gaccgaccga
    50341 ggataccccc aggcagaaaa aaaaagcatc atacccggta cattgcgcct ttattcccga
    50401 taggtcaaag aaatccggga gaatgatttc agtaagtttg ggatcccgga taccgcaggg
    50461 tttacggttg atgaccagaa tggattctac tgcggtatgg tgcaggcatt catgcagaac
    50521 cccttccccc accatacccg tagacccggt gacaatgact ttcatgattg attattcttt
    50581 atttaccttt taaaaaagcc aataagccgg ccatatcctc aacggtgaga tttccttcca
    50641 atcctaccgg catcagggac attggctcag actcgaggag ccggatttcc gtcctggcga
    50701 tcagggttgc tgttcctgca gcgtctttga gttccaggga actctgggtt tgtgcggata
    50761 tgatcccgct cagtttttcc ccgttctttt tttcaatggt ccaggtttga tattcatccg
    50821 caatggaacg gttggggtcc agaatatccg ccagtataaa tccggcttcc cggttgcgaa
    50881 tggtagccag gtcggggccg atggatttac ccagggcgcc gtttacctgg tggcaaatcg
    50941 cacagtgctg catgaataaa gatttccctt gaaccggatc cccttccatg gaaagggctg
    51001 cccggaattt ttcgtaaacg gcattcctgc tttcttccgg atggcccagc agtctccggg
    51061 aatattctcg aatggaatcg gtataactgt tcatcaggcg cacggtcctt ggccagccaa
    51121 tcgaactttt ctggatctgg cccgtttcga ccgctttcaa caaggcggac atcccgggcg
    51181 gggatttcat caggagctga agggccgctt cgcgtacggc gggcgtaagg tttttccagt
    51241 gctccaggct ataagcacgg gcggcctggg gagcaatatc ggtaaaggcc tggagggcgg
    51301 cttcctgaat ctcatccgtt tcgcccggcg agagcaggcc ctgtattaaa aggacatccg
    51361 aagtatcctg gtttaatccc agaattttta tggcatcctt gcgaagaccg gcgggagaag
    51421 cgggatccga aatgaatttc cttgccctca aggccatcgt cgatttttgt tcaccctcgg
    51481 gcaggcccaa aacggtaaac aattggacca ctgcggccct gatttcagca ggctggtttg
    51541 cctggaaaga accgggcaaa gcgtcccggt atatttttac cgatgaagga tcgggtttat
    51601 tcgttcttat tcccgaatac aatcctttca acaaagccgc tttccaccag gcggacggct
    51661 ctttgtctga ttgggtagcc aagcccatag tttggatgat cgaagcgggt tctcctttca
    51721 gggcaatgcc cgctcccaat tgttcgaaaa attgttttcg gccactggtc tccctagtcc
    51781 ccagttggcg gaccatctca acgaccatat ccgggccaaa gcttccggct gcggtaagta
    51841 cggcgatctg cacccatcgg tcttcaatat ccatgaacag aattttacgg atggcccttt
    51901 ttgcttcagg atggttaaaa ctgcccaggg tgcaagccaa ttgaaatcgg acttttgaat
    51961 cgggatcctg gttcatgctg accagagtat tccataaaga ctggaccaaa cccccatcct
    52021 cagccagccg gatcgcattt tctcttaccc gcgggtgagg atgttccagg gccggcttga
    52081 ggtcttcgga tacgagatta ttcattcccc ggagggtcca aagggcatgg actgtccctg
    52141 ccggcgacat gttcccggtt cggaggatag tacgtatccc taatgccgcc gcggtatctt
    52201 gtttatctag aaggaggcgc tgggcttgtc gccgccacca tttgtttttg tgatcgagca
    52261 gcgctgccag ttgttcacca ttcatggatc caaggcttag ttttccgcac caatccatgg
    52321 gcggggtacc ggaggggctg atgcggtaaa tcctgccctg gtgactgccg ttgtacaagg
    52381 tgccggattt attgacttca tcgctcatcc attccggatg ttctatgatc tggcggtaat
    52441 aatccagcac aaacagggcg ccgtcggggc caatataaaa ttgacagggc ctgaaccagg
    52501 catcccggga agccaggaat tctttttgtt catacaccct ggaagccgtg aaggaggcac
    52561 cccggtccgc gatacgatcg gcatgcacca ggttgtggac cggctccgca atgaaagtaa
    52621 cattggaaaa actgtccgga aacaacccgc cgtcgtacca ggtcaccccg caggaagaag
    52681 tgatcacgcc aacatccgtg agcaattgat gttccgggtt ttgggtaatg ggatatactt
    52741 cacaggcatt tccgtgatcg ggtatgtttt ccgtggcatc cgcgacgagc agggtggaac
    52801 ttcgctgcaa atacctcgcg gctatgattt cactgaacag atggtcggca ttgctggtca
    52861 gaaactgatg gccccaggga tcaaaggtct gaccatattg ggattcgccg gccatcattt
    52921 ccagcaggga ttcgtccggc ttaaaccgga cattccggcc attggcgtca gcaggcagga
    52981 cggggcctcc ggttttagca ggatagagta cggcggaacc cgtatctcca aattcttccg
    53041 ggaaaacctt tggatttacc gctccctggt gtgccaggta gatccaattg tccagcccga
    53101 aaaccggcgt gtttgcaatg tgctgcgggt ttgtaagggc aaaaccggtg agcaggatgt
    53161 cccggatatc tgctctatgg tccccatccg aatcttccag gtaaagcaca tgagggacgt
    53221 ctaccaccaa aaaacctttt ttccaccgca tgattccggt gggcaaaacc aactgatcgg
    53281 cgaagaccgt gcttttatcc ggccgaccat ccccattgct gtccgtcagt ttttttatga
    53341 ctcccgagcc gcttttatcg aggggatagc cgtgcatttc gacgacatag atattgccct
    53401 cttcatcgag gtccatagcc accgggtcgg aaatcagggg ttccgcggcg accaattcca
    53461 tctggaaccc atcggccagc tcaaaggtgg taagggcctg ttccggcgga aacccgcctg
    53521 gagctggttt attctgttct ttgcaggaaa ggatgccgag gcaaaataag agggagataa
    53581 atccggtttt ggtcacgcga taaaggttga tccaaaataa ataaaaaacg gaaatatact
    53641 gcttcagaca tccggtttgg tccggagacc cctggtttaa gacaaaggaa gccagcctta
    53701 attaatgtct ttattttccc tgagccttga caggtttgat tccagctggg ccctggcccc
    53761 cggacttgct gcctgggtca atgctttctg gccatatttc agggcatttt cgaaatctcc
    53821 ccgggcggag taaacaaaca taaatcccag gttgaccaga taaatatctc cgtgtttttg
    53881 caggctggct tgctgaatat caagcgccct tccggtcctt ttctgcgaga tcagggttct
    53941 gctatacgag gtgtactgag cggccgtagc catggtcaag gcttccgtca tgacactatc
    54001 cgcctcggtc atccggttta atttttcaaa aacagcagcc aggttccgga ggctgaaata
    54061 gcttctctgg ccctggaacc cgccgacggc cctctggctc cagagcaagg cctcgtccag
    54121 attgatattg ttgttcagac aatattgggc tgcctgtatg agattgttgc tgttgaatcc
    54181 tttctggctg gtgacctgtt cgcgcaagcg tgtcaaaacg atgttttcga catccacttc
    54241 tattttaaat ggaatgatcc atttttccca aagcaaggcc acggtgcagc tgttgggttt
    54301 atgatcgata aactcatatt tgagccactc cacgtgttga tcatgcttca atggttttac
    54361 gatgacccgc aggacgtcgt ttttttcttc gtaataaaag cttccccagg catcgtgctg
    54421 ccctgaaagg atgagcagga cgctgtccgg ccagactgcc atatgaatcc cataggtacc
    54481 cgccgggacc ggcctgccct ccacgagcac gtcgtgttca aaccggatgg ttgtattttc
    54541 attggctccc gcccgccagg gtgcggtgtt tccgttggtg ataaaactga aggttgaaaa
    54601 tccaaagggc accagggttc cccagatctt cccttcacgc ccggaaacat ccggcctgga
    54661 gtaagtgagg gtgatgccgg tgatccccac ttcctcagac aggattgccc gcggatttcc
    54721 tccaaccggt ggaaggtcca tctgggcctg ggcagcataa atggaaaaaa gaaaaatcag
    54781 gaccgaatgg accgttttca tttgaatctg ctcatttgct catttactca ggagtataaa
    54841 ttaaatctgg tttctttaag cccgagttgc tgaagataag ccgccagttc gccggcttct
    54901 tcaccccctt tgtttgcatg gtgaaggagg gaaaacccat gggggccttt catataaaga
    54961 tggcttgggt acaattcaag gtatgacttc atccaggcgg tttgtcccag catggtgagt
    55021 aaaaagatat tggtccttga tcctttttcc agtaaaaagg cgacaatgtc ctttctgcca
    55081 acatgccctg ccgcttcgat gcccgattca aaatcgccgc ccccccaatc ccaggatgca
    55141 aataccaggt ttggaaattc cgcctggagg gcttttacct tatccagatc ggaatgacaa
    55201 acacctacga attccttgac cttttccaca ggcaggcttg ccggtttggg gttgttctgg
    55261 ctttggagca tggcggatgg aaccgctaaa cccagggaac cggctgccat ggaccggata
    55321 aatttcattc tattcatgtc ttttggattt ggggattcta aagctatttt attttcagct
    55381 ccctttcccc ttgctccggt gttttaacga aagcttaacg ggaccgcttt taataggaat
    55441 aaatcagtat ttttgccgtt cattaaatca agataacatg gcagaaccca tcagagcata
    55501 taatgtgaaa accaaagaaa aaaacgtgga aatgaaggat gccgtcatca ccaagacggc
    55561 tcgcggaggt tatatggccc agggcaatga cggaaaaggc aacaaactga ccagcctgct
    55621 cagcgaggcc aaagccctcc aggcgattca ggacggagtc gctaaaaaag gctggtaatc
    55681 tcttttacgg attacctaaa tcatcacttc agagattgcc cgccgttcgg gcgagatcat
    55741 cagcgggcag cggataaaat gcttccattt tgagctatta tcctgctatc ggaatatatt
    55801 attgtgagca gggcaggtcc tgatcctggg atgcgtttcc gttttttcta tctttggtca
    55861 tgatccttgc cgtctttggt attctcctgg ttttggtatt gctgataacc tgggcgaaga
    55921 tccatccgtt cattgctttt ttaatggtct ccattctggc gtccctcctc ctgggagtga
    55981 atccggggca gattggtgct tctgtgcaga aaggggtggg ggatattctg ggcccggtgt
    56041 tttcggtgat tatattaggt tccatgctgg gtaaaatcat tgcctccagc ggggcggccc
    56101 agcaaatttc cgaatccctg gtcaccctct tcggccaaaa aaacctggct tgggctttag
    56161 ccctcaccgg attcatcgtg ggcataccct tgttttacaa cgtggggttc atcctcttga
    56221 tccccctgat ctattccctt gggcagcggt ttcagttgcc gctggtttac ctggcggttg
    56281 ctttgtgctc ttccctgtcg gtgacgcatg ggttcttgcc tccccacccc tcgcctaccg
    56341 ccattgtgcc catgatgaat gccagcctgg gacaaacctt gatttttggc ctgatcgctt
    56401 ccatacccgt gatcttcctc gcaggtcctt tatttgcaaa aagccttcgc aacattcagg
    56461 gatcgccgct ggccagcctt aaggtgaaga ttttcgaaaa agccgaaatg ccttcaactg
    56521 gcatcagttt tttttgcgcc ttgctgccgg tggtcctctt ggccctgggg acttggttta
    56581 cctctatttt tgagaaaaac agcccggcct ttgagatttc cagcctgatg gccgatgcca
    56641 atatcgtgat gctgctttca ttattgctgg cctattattt cctgggcatg cgaaggggcg
    56701 attccctggg caaggtgatg caggagggca ttgaatctac caaagacgta tttcccatac
    56761 tcatgatcat agccggagcc ggggttttaa aagaaatcct gatcagcgcg ggcatcagta
    56821 ctcagatcgc gtcgatcatg aaaacctcca gtttttcccc gctggtcacc ggatggttca
    56881 ttgctgccgt gatccggctc agcctgggca gcgctacggt ggcgggcctc accgccgccg
    56941 gcctgatcgc tcagggcttt gaccctgcag tggtcaaccc caatttgatg gtgctgtcca
    57001 ttggcgcggg aagcctgatg ttttcacatg tcaacgacac gggattctgg atgtttaagg
    57061 agtattttaa tctgaatatc cgggacacca tcaagtcctg gtccctgatg gaaacgatcg
    57121 taagttttgc cggattgatc atggtcctga tcatggatat ttttgtttaa ttcctcattc
    57181 cgaaatgatt tttgttgaaa cccgaatatc agcctgaaaa aatcagagac cgtttcctga
    57241 aaaaccaaca ggcgtataaa aaattcctgg cccgagcccc attaaaaaaa gggattccaa
    57301 aactggccgg ccttgctcaa gaggtgtatg cgcgccatga ttgtctttcc tgtgccgcct
    57361 gttgtaaaaa ttacagcccg cggttcaaag cccctgacct gaaaaggatc agcaaatacc
    57421 tcggcatgaa agaaaccgaa ctccaggacc tttacctcaa aagggacgaa gacggcgatt
    57481 atgtaatgaa aaccgccccc tgcgcctttc ttggaccgga tcaccggtgc agcatatatg
    57541 aaaaacggcc ctcggattgc cagcggtttc cttacatcga tgaagatgtt ttcttaaaac
    57601 gaaaagagct gacgctgaaa aacctgtcct tctgtcccat tgcccaggat atgatggaaa
    57661 aactgatgga acggtaaaaa gcggcaggat atatgccagg ggcttacctt tgagcatcaa
    57721 gaaacccgca gcatgaaacg gaagctcatt ctcctcctct tttctttttc tatcctggcc
    57781 tgcaaccggg acgaagtgga aggggatccc atccccgcag acctgactac gcttttacaa
    57841 ggcagttttg aatcggcggc ccaccccacc agcggcaccg taaaactggc caaagacgga
    57901 gccggtaaaa aatacctggt atttgaaaat ttcaaatcgg atccgggacc ggacctccgg
    57961 atatggctgg cagaagataa atctgcaacc aactattcag aattgtctac caccgttcct
    58021 tcagggaatt ttaaaattga cgtaccccca ggagcggata ccagcacccg gacttatgtg
    58081 ctgatctggt gcaaagcctt caccgtattg ttcggcagcg cacaactaaa atagtcgacg
    58141 gaaaagggtt tggggaaagg ccctactttc catctccttg gctggaaatc ttgttccgga
    58201 tattgtctat caggcgggac gtcacatggg gccgctgaac tacattcttt ttaatcaggt
    58261 ccctgaaatt ctgctcggta cggagtaccc gctgaaattc aggattttcc tgggcctggt
    58321 gtaacacttt ttggttttcc tcgtcattca actggtggtc aagaaacata ttgaccatcc
    58381 ggtgatattc atggatcgga tccggatttc tcatacgaca aaatcgttag aatgcaccat
    58441 tatttattct tcaagtttgc gagacctgga ttttcgcttg tctgaaaaac ccatggtttc
    58501 ggcatatgct tttaatttat cttttaacac attccgggcc cggaaaagcc gggaacgcac
    58561 cgtaccgatg gggacatcca ggattttggc aatctcttca tatgtaaatc cttcaacgtc
    58621 acacaataat attactgtcc taaaatcaac gggtaatgaa ttgatcgcat tcgtcacttc
    58681 atcccccatt accgtgttaa aaagctcttc ccgcaaatcg gtatattccc cgatcgcact
    58741 ctcctcactg tcctgatatc ctataatatc ctcgtaatct acatgagccg gagccttgct
    58801 cttcctccgg tattcattga tatacgcatt tttcaatatc ttaaacaacc atgctttggc
    58861 attagttccc tccaggtact tatctataaa gcggtaagct ttaagatagg tttcctgcac
    58921 gaggtcgtcc gcatcgtctt cattgagggt gagatggaag gcaaaagtgc tcagggcatc
    58981 aatatggggt aaaagttctt tttcaaatac ccggtcgtag cgtttccgtt cttcttcgtt
    59041 cacaggataa aggtgataaa aatcccccat atcagggggc tggcctttca attttgttta
    59101 ccctgccggt atccattaaa gcggcattct tcgcattctg aattattagg tctccctcct
    59161 tattccttag cgtccggttg acggagcaat ccggcataca cccccctttt gtcggtgttc
    59221 ggaatagtgc ggctggtatg gcaaaatcat tatccgggcc atctcttccg gttggataag
    59281 cccctttcat tccatggtgg tttcattcaa atacgggtca ccggtcctat ccgcattccg
    59341 gatgatcttt cctgatgcgt gcctataaac ctttccttcc tgaagatgac aaagacgtac
    59401 taaaaaatat gaaattattt ttatcaatat ttggtttata gtgattatta atatagattt
    59461 attgctttaa agttttaaag acattataac caacacgaaa actttcttgg aaactgattt
    59521 tacatcggat ctttcaactt ccacccttct ttccttaacg ctttcatttc ttccattgat
    59581 cccggcggga gctgttggct tatatcgtac tccggaaaca tcatttatta tatatgaaac
    59641 tcaaaacagg caaatcacaa gtcgagttac tcagccgcca tggtctgggt aacatctatt
    59701 atgatcacca cacccagcaa aggtcattcc aggcaaccaa aaacatcaaa tcgggacaaa
    59761 ttctttaccg cttcggaccc aaacaaatcc aggcgaatcc gacctatttg acggttcagg
    59821 tgggcgaagg ccagcacatg gtgctcgatc cagaatttct ggcggccatc aatcacggtt
    59881 gcgaccccaa cgtttttttt gatgtagacc acggcgttct gcgtagcgtc cgggacatca
    59941 aagcgggaga acaaatggtt tacttttatc cctccacgga atggcaaatg atccagccct
    60001 tcgattgtac ctgcggcagt cctaaatgcc tgggttacat tcaaggggcc catgccatcg
    60061 aacctgagct gatccagcgt tatcatttgt cttcctacat tcaaagccag ctcaaagaag
    60121 tgcccaccct ggtctaaaaa ggcccagcta ctagactttt catgaaatcc tttcgcagat
    60181 tcaagaagct gacgggcaac cggaaaatca aggtttgggt gcttttccct tatttgaaaa
    60241 ccaacgatga acagttggat tattattatg attttaatca aagcgtcgcc gagtataccc
    60301 gggtgtttga aaaaatcaaa atgccgtgga cctggcagcc cgtgacgctg gaccattacc
    60361 gcgaagtcat cgaaggcctg cagcaggaac tgcacgaagg ggaatttttc cccgtggtat
    60421 taaacctttg cgacggcgat gaaataaacg ggacgccggg tctttccgtg gtcaaggccc
    60481 tggaatacag cggcctggtt ttcaccgggg cagaccattt tttttacgaa atcactactt
    60541 ccaagatttc gatgaaggaa gccttcgatg cccatggggt ctccaatgcc ccctggaaaa
    60601 cgatccaggc gtccaatccc agccctgccg gtattttcaa aagactgggt aaacccctta
    60661 tcgtcaaacc ggcggtctcc ggcggatccc ttggggtagg aataaaaaat gtggtgaata
    60721 ccccttccga aatgaagacg gaagtcctca aactgtttga aggttaccgg gggtgggaat
    60781 tcggcgccgg gggggtgatt gcggaaaagt ttatcaccgg accggaatat accacactga
    60841 tagtaggttc gtatgaccgg cctgaggacc ttatctactt tgcgccggtt gaacgggttt
    60901 tccatgcctc cctgcccgat catgaaaaat tcctttctta cgaacgcctg tgggaagtgt
    60961 acgaaaacga aagcagcatg cctttgcagg gtaattttta tgaatataaa tcaccccctt
    61021 ttgaaatcca ggatgccctg gaagccatct ccttgtctgc ttaccaatcg gtccggggca
    61081 agggatatgg ccggattgat atccgcatgg accagacgag cggcaaattg tacgtgctgg
    61141 aggtaaacgc gcaatgcggc ctgagtgaag atgaaaactt tacctccatc ggcgccattc
    61201 tcaaatattc ccaaaaatcc tttgactatc ttatccggga aatcatcctg gacgccctga
    61261 tccggtttga agtggaagcc ggtgtccgcg cgaatgtcct ggttggctga tcaaaccatg
    61321 aaccctgacc ttttccgttc cgtttttaga tgaaagtctg tgtcttacag cccgattatt
    61381 cggcttcttc cgtggattat aagtattatg atcccccccg ggatttatcg cgcctgcttc
    61441 cggggcatac cgtggaccat gtctttctga acaaactgca tacctacggt caattgagcg
    61501 ggcttcagca tgcaggctat gatatttttg tcaatttatg cgaagggtac ctggactggg
    61561 atattccttc catcgatgtc atttatgcac tggaatccct caaacttccg tttaccgggc
    61621 ccacgagcag gttgtacgac ccctcgaaag aactcatgaa atacgtggcg tacaccgaag
    61681 acatcctcac tccaagatat gccctgatcg agcgcccgga ggatatcccg aaggctgtgg
    61741 aaaaacttca atatccattg tttgtaaaac cggccaaagc cggcgacagc cttggggtgg
    61801 atgaccattc cctggtcgcc aacccggcac aacttctgga aaaagcccga gcgttgttgc
    61861 tggaatatcc gtccctgctc gtcgaagaat acatcgccgg gcgggaattt accgtcctgg
    61921 tagccgcgga cccggccaac gagaaacaat gccgtacctt caaaccggta gaatatgttt
    61981 tccccagtgg ttttgcctat aaaacctatg ccctcaaaac atcggaactg cacctggagg
    62041 ccaatgttcc ctgccaggac cccgaactcg aaaaccggct aaggcttgcc gcccagcgaa
    62101 tattcagggg attcggagga atggggtatg ccaggcttga tttcagggtc aacgataaaa
    62161 aggagatcta tttcctggaa atcaatttta cctgttcggt tttttattcc gatgggtatg
    62221 agggatccgc cgattacatc cttcgttttg atggcatcgg tcaggccggc tttttgcagc
    62281 acatcatccg ggaagggatt tccaggtaca aagcccagct taaaaagtat acgatgaaag
    62341 gcgattccat caatggattc ggcatttatg ccaacgtgca cctcgccgca ggagaagtga
    62401 ttttcaacaa cgaagcccgc ggccaccgga tcgtcaccaa acaatttgtg gacaaaaact
    62461 ggtcggctgt ggaaaaagac ttatttgccc ggtacgccgt gcccctgagc ggacacctct
    62521 atattctctg ggatgacgac ccctccgtct gggcccccca gaatcacagt tgtgatccaa
    62581 ataccggcta cgacgggctc aatgtcatcg cccttaaaga tatccctgtg ggcacggaac
    62641 ttaccctgga ttacgctact tttctggatg aaaccatgga accctttgcc tgccagtgcg
    62701 gctccgatca atgcagggga tggatcaagg ggactcccgg caattcgatg gaaaaaaggg
    62761 aatccctgag agactgatca ggcattttta tcctctgagg gcttgtcgct tttgaggttt
    62821 tcggtaaact cctggatctt atcgcccacc acctcggaaa cggctcgtac ggtatccacc
    62881 acatcttcaa gcgtctctcc aagcgtggtg ccgatggcgt ccagaatgtt gtcatgcggt
    62941 tcaaccggct tgggactccc ggaaatggcc tcggcggcct tttcaaggcg ggtatcgtct
    63001 ttccttgcct cggcggcctt tttaatctcc tccttaatgc gatcctcgac ggcatgtttc
    63061 gcctgttcca gttttaattt ttcctggcgg cgtatttccc tttgtttttc ctgttcctgt
    63121 cttttttcga gatcctcccg ggtagatctc atgtcgtgaa gtttttcttc cttggaccga
    63181 atgcgttcct caatcatttt gattttggcc tgacggattt gagcttccag ctgcccttcg
    63241 gaattggcca cctttctttt ttcaaattcc aggtcgttgt aatccctttt gatggaggat
    63301 tccatttttt gcagggcttc gataaggcct tcgtaccttc ttgaaaggcg gtcagacccg
    63361 gaaccttcac gcgaatcctt gtgcccgaat cttttgtcct tgatttgttt gaacagttta
    63421 tcgatctggt cccagatgtt actccggtca ttgcggttca tctcaatccc cttcaaggcg
    63481 ttttgcaatt ccttcagttc agaaaaaaga ggctgcaggc tgaggttgtt ggaaagttta
    63541 tgacttattt catccagttt tttctgaaat tgatttacat ggtcccggga ggtacggtca
    63601 aattcctcat cgacggcctt tctcaacttc ttcagttgct caaacagggc attggtgccg
    63661 tcacgcagga tctgggcatg ttctttaaac aggttttttt ccctgacctg gttttgaacc
    63721 cggtcccaga aggttttgag ctcttcccaa agctcgttgt cgtatttgag gagtttatct
    63781 gttttttctt tcaggtcccg gtattcattc tcaaagccct gctttaattt atgggaaagg
    63841 acgataaaat gccattcggt ctgggccctc aggatgatat cttccttatc cggctttaaa
    63901 ccgggtgata aaagagactc tgaagccgta agggcttttt ctgaatggac cgcagattcc
    63961 gggaaatcga gctcttttcc tatcctccag tccctcatca tggtattggc aaattcttcc
    64021 gtcacatggg cctggggaaa aatccattcg tgtacaatat tttgttccgg atcaagctgt
    64081 aaagcaacca gtactttttc gttctcagta ttggtacccc aaaaaacaat tctccttttc
    64141 atacggtaaa atcaggtaat cgattaacag ttgcggtaag ttaggtttcg tcctctatac
    64201 tgtttccgct tcgagcggca gtagttcttt ttttaacata tattctgcga tctgaatagc
    64261 attcgtcgca gctcctttgc gtaaattatc ggcaacaatc cacatattta aagtcctttc
    64321 ctgagactcg tccttgcgaa gacggccaat aaaaacttca ttcctccctt ttgcatataa
    64381 cggcatggga tatttaaaag ttgccagatc atccatcatt tgcacccctt cagcctgaga
    64441 aagtaacttt attatgtcgt ccatctcaaa atcccgttcc aattcgacgt taacgctttc
    64501 agagtgaccg cccttgaccg gcacccttac cgcagtggcc gtcacccgga tcccgggatc
    64561 tccaaggatt tttttggttt cattgaccag cttcatctcc tccttggtat acccgttgtc
    64621 caggaacaca tcacaatggg ggatgacatt ttcaaaaatc gggtatttat aagccttttc
    64681 ctcctggggc acctgctcgc ccgaatgttc ccggtcgtat tgcctgaccg ctttcacccc
    64741 ggtaccggtc accgattgat aggtcgaaat gaccagcctt ttgatgccgt atgctttatg
    64801 aagcggcgcc agggccacca ccatttgaat ggttgaacag ttgggattgg caatgatccg
    64861 gtccccggca gtgatcacct cgccgtttac ttcagggacg accagttttt ttgaagggtc
    64921 catcctccag gcggaggaat tatccaccac caccgtaccg acctcggcaa accggggcgc
    64981 ccattccagg gaaaccgatg caccggctga aaagagggcc aggtccggtt tttttgccac
    65041 cgcctcggcc agggtatgaa tcttatattc ttttccttta aacaggaatt tgttcccggc
    65101 cgatttttca gaagccacgg gaataaattc ggtcaccgga aaatcccttt cttcgagcac
    65161 ctggcacatg acggtaccga ctaatccggt agcgccaacg catgcaactt tcatatggag
    65221 tacaatgata attaagcccg caaatataca tggtttcaaa atgcagttta tttttaggtc
    65281 agagacttat tatatttaat tttttaatat aagtttttaa ttaaacacac accctatgag
    65341 accactccat tccctgcttg cagggctgtt cctgtttgtt tgcggcatcc gggttcgtga
    65401 attttttttg cctgatattc ataggaataa aaccagggtt tcccctgacc ctgttctccc
    65461 ctttgatttg ctgatcagtg aaatcatggc tgatccctcc ccggtggtcg gcctgccgcc
    65521 ttatgaatac ctggagatca ttaaccactc cggaaggacg ataaacctcc acggcctgac
    65581 tttgagtttt gaatcccgtg cgttccggat tgaccgggat gacctcctcc tggaacccgg
    65641 gcaatatttt atgttctgcg aaccggatgc ctttccggct ttgagtccct tcggacccgt
    65701 cctgggactg gaggcttggc cggggatccg caacagccac gggaccctgt ccctgtatca
    65761 tcaaaaccaa ctgattcaca gggtatccta tactgatgaa tggtatgggg acggacggaa
    65821 gaaaaatggg ggctggtccc tggaaatgat caatacttcc aactggtgtg accagattaa
    65881 aaactggact gcgtccaccg ataccaccgg aggcagcccg ggcaggaaaa atgcggcagc
    65941 ggattcaaat tttaaagtgg agcctgccct ggattcggta gtggtatccg gcgaccaaaa
    66001 gatcatcgtt tatgtaaaca aggatatcca ggagccttct gtctcggatc ttgttttcag
    66061 tcccccaatg gatgaaacca ggatcgtccc gggtgacgac cttcagtctt tcatgatttt
    66121 gttgcctgcc ccgcttttgt ccggccaatt gtaccagatt tccatacggc tccgggattg
    66181 tgcaggccgt aatgccggcg tgcactcctc cattctgacc aaaccggaaa aggcagaaag
    66241 gaatgacctg atcatcaatg aaattttatt caatccggaa ggccgcggtt ctgattttgt
    66301 agaactgtac aaccggtctg gaaaagtggt gttcatcaaa ggctgggtcc tcgaaaacaa
    66361 aatgaacagg cagaaaaaaa cgatcggaac cgctgcatac ctcctgcctg gcggatatct
    66421 tgtgttgagt ccggatccgg tggatattgc tttccggttt aaaaaagttc agcccgacgt
    66481 gatgattgcc caggatttgc cggccctggc cgatggttcg ggccacctga ccatttattc
    66541 gcaaggcgga ttgctggttg attcggtata ttatgaggaa acctaccatc atccttttct
    66601 gaattcaaag caaggggtat ccctggaaag ggtcaatccg gaagccgggg cggatcgaac
    66661 caattggcag tcggcttccg gactggaagg ttttggaacg ccaaccagaa aaaattcagc
    66721 ctctgtttcc gttgccggga gtgaatattt ccaattaaaa tactcccttt tcagtccgga
    66781 cggagacggt taccgggatc agcttgaaat agagtaccgc ttgccccgga acggatacct
    66841 tggcagggtc tatatctacg atgaccgggg gggcttggtc aaaaaactgg tcaataatct
    66901 gtctttttcc cagcaaggaa gcatcgaatg ggatggccgg gacgaggccg gaatgaattg
    66961 tccgaccggg atatatatcc tggccgcgga agccctggat atgcagggga atcatctgca
    67021 aaaaaaatta gctgccgtct tatcaaggcc gggataatcc tgataaataa atcatctgct
    67081 catttcgttg atgcaatgaa gattaacttt gactcaggaa agccatcttg aaaagaatta
    67141 aacaactgct gacataccaa tgggtggaag gagggatcat cctttcgatg attttgataa
    67201 ccctgctttt attattcact cccaaccggg ccaccctggc cggtctgagc agtcatgctt
    67261 ttgaagcggt gcttcttttt ctgggttccg caatgggatt tatgatactc gacaggccca
    67321 agattatgtt tacctgcctg ggcatgtgtg ccattttatg cctttatttg aaaaaaacca
    67381 gtaattccag cattcaatcc ctgttcaggc aggcggatat cgatttcagt ctttgtcaaa
    67441 tcctgattaa ttccggggcg gacgaatacg aaaacatcag ccggcgactc ctggccagca
    67501 aggccgatat cctcaacatt caggaactca cgcccgactg ggccctggtt ttatctgatg
    67561 gtttgagcga aaactatcct taccgggcag aaatgaacag ggtggacccc tatggcatgg
    67621 tggtttattc caaatatccg atcctgaaaa cggataccct gcatttcagg gacacgatgg
    67681 tcaattacga cattccggtg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    67741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    67801 nncagccctt cgaacacatc ctttattctt cgaccatccg ttgcaataaa ctggaagaag
    67861 taaggacccc cggtggtctg catcttggga tacaaggcta ttacactttt aatccttccg
    67921 gggaagacct cggggtcaac aaaaattatt aaaccccttg agcagtaacc ttcatacctc
    67981 taaagaactt aaccccttga acgaagccct ggccgtgcac tgcaaaccct ttgcggaact
    68041 ggtcgccgcg gaactttatg atatcctccg gctgcggcaa caggtattta tccttgaaca
    68101 atcctgcctt tacctggaca ccgacggtta cgacgaggcg gcccatcacc tgatgatcta
    68161 taataaacaa aaagacctgg tcgcctattc caggttgttt ggtttgaata tgccctatgc
    68221 cggctattta tccatagggc gggtggtgac gagtccccgt ttccggggac tttcatatgg
    68281 gagaatactc atgcggcatt ccatcgaaca ggtcaggtcc attcacggac ctcacccgat
    68341 caaaatcggc gctcaggcct atctcattgg tttttattcc tctcttggat tcagaagcac
    68401 cgatcagcgc tacctggagg acggcattcc gcatctgaaa atgattttgg atctatgaaa
    68461 agaaacttaa ttctgaatac catgcataac ctcatcggat tatttatcct gattacaggg
    68521 atttcctgca attccagccc taaaacagac gaccctccgc cttataaatt ggtatggaac
    68581 gatgaattcg aatacgacgg attgccggac agcaccaaat ggtcttataa tgtgggaggt
    68641 catggttggg gtaataatga attacaattt tataccctaa aaaggctgga aaatgcccgg
    68701 gtggaaaacg gcctcctggt catcgaagcc cgtaaagaag agtgggaagg aaacccttat
    68761 acttcagccc gcctgatttc tgcccaaacc ggactggata aaccttatgg gtggaaatac
    68821 gggaaagtgg aggtgcgggc caaattgcct gaaggccggg ggacctggcc ggccatttgg
    68881 atgctgggcg atcctcaacc cctccaatgg cccgatgacg gagaaataga tatcatggaa
    68941 catgtgggat gggatcaggg ccgggtacat gcaagcattc attgcaaaaa ataccaccac
    69001 agcatcggta cacaaaagac tgcaaacatt tccgtacccg attgttccca acaatttcac
    69061 cgctatgggg tggaatggga tgccgaagaa attcgcgtct ccatggacga ccaggtttat
    69121 ttcagtttta aaaacgaaca ccattcgtat gaatcctggc cctttgataa taaaatgtac
    69181 ctgctcctca atattgccgt tggcggattc tggggcggcc aacagggtgt agatgaaaac
    69241 atctggcccc ggaggatgga aatagattat gtaagggtat ggcagaagta gtgggcccag
    69301 gccgaccgcg agccatgcct cgcccatccg aagcacatca aaaaaattct aaaccctttg
    69361 gaaaaatgaa aggactgtac tttatctgga tttcaatggc ctggtccggt ttgacctggg
    69421 ggcagcaatt tgcaagacag gtatttattc tatcggatga attttcgaaa gaggcctgtg
    69481 aggtgattgc cgcctgcgac tgctgtgctt cagaccttat ttttctgaat aaaaaaaaat
    69541 ttgccctggt cgaccggtgc ctccatcacg acacctacct ccggggtaaa tatgccctaa
    69601 aaggaaaaag catgaccctg gacttcagtc cggaagcggt gagcctgcgg tatgatgaaa
    69661 acacccaact tataaacaag gaaaaaaaac agatgagcat gtctcccctg atattgcaga
    69721 tcggtcgttg cggacaggat gaggtgaccc tcggacatcc caccatcagg gaatataaat
    69781 acggttcccg gcgcaaagga ataaatgaaa aagagatcct ccggcaaata aaaaagtcca
    69841 agccctggaa atgacccccg ccgacaggtg gtcaacgggt ttaatacctt tgtaagacct
    69901 tgtccatatt cctctacgat aaaaaaaaac agactgccaa ttccaaaatt caacctcgtg
    69961 aacattcatg aaaaatctta aagaaaaaat ccgttccggc gaggccctgc acggatgctg
    70021 gatcaatctt gggtccaccg tatccgcgga aatcatcgga agagccggtt tcgagtgggt
    70081 cctgatcgat ctggaacacg gttcgggcaa tgacctggcg atgtaccagc aattgcaggt
    70141 cctcgaaggc acccccgcaa cgcccttggt gcgcaccgat gaactttccc ggtcaaaaat
    70201 ccaacgtatc ctggatgcgg gggcttcagg gatcatgttc ccccagatcc gccaggcagc
    70261 catagcagat gaagtatgcc gcatgatgta ttatccgccg aaaggcaccc ggggtatggc
    70321 aaaaatggta agggctacgg gctttggtaa aaatgcaacg gagtatattc atactctgga
    70381 aaacaatctg atcggggtgg tccagattga aaccccggat gccctcgagc aggtggaccg
    70441 catcgcctcc ctggacgcgg tggacgtcct gtttgtgggc ccttcagatc tatccctgtc
    70501 cctgggcgta tttgggcaat tggatcaccc ggtgtatcaa aaagctataa gggatgtggc
    70561 ccttgccgct aagaaacacc ataaagccac aggggtttta ttgctggacc ttgctgaata
    70621 tgaaatgtat ttccaactgg gttaccgttt tctggcttgt ggagccgatg ctacttttgt
    70681 cgcgcgcgga gcagaagcca tggctcaagc cctgtcgaaa aaaagatccg gcgatcaggt
    70741 ttaaaactca tctccttatc cctatgcgtt ttatcctccg gccaacgacc gtgccgtccc
    70801 tggagtgaac ccgtccgcta caccaaacga tctataccgt ttttatatca acggtaagcc
    70861 ccggaccagt ccaggactac atagtcaacg cttgctttca ccccgtggtt tgtctgacta
    70921 tctgtaaaaa cgaccccaaa ttcattcgtg ggctgaacat cgacagctat ccgtcttaaa
    70981 tgatgatcgc cgtccttgaa ttcgaaatta aatccggcca acaaagcatt gcccctgttg
    71041 gtcttgggga tccgggctga atcggtaaat tcaaacgatt ggctgtaaac ccccctgagg
    71101 attaacccac gcgggtgatc ccctcttttc aggataaaat accttatgat gtaagcatac
    71161 cggtcatcct gcggagagtc gtccacaaaa tcaactctga tctcgctgcg aatggaatga
    71221 taccggaccc ccactttgcg aaggtggtgg ttcccgccct gcgttccctg aaattcaaag
    71281 ccccgtagaa caaaaacttc atccgctgcc agcctgggaa tggagacgat gctgttttgc
    71341 cggtttgaag aagatccgga aatgctccgt tcactggtcc gggtcaggtg agggggggtt
    71401 attggatagt agaccgcttt aagttctacc gggtcccgtt gattgcccaa aagaccatta
    71461 tccccgtcac ccaggcccag taaaaaactc gacggttctc ccgttttccc tcccgctata
    71521 agagtttgca catgatggtc tccgtttaaa taagccaggt cgaatgcagc aagtgccggt
    71581 atatccgtgc cggtaagatc cagaaaaagg gcatcggttc cccgataacc ccatacaccg
    71641 cttcgaatgg gtattggttt ataactcata agggagattt actctaaata tagaggatta
    71701 cctacgcatc ggggtaaaac gcaaacattt gtcacatgat gtgtttgttt tacccgggcc
    71761 tgatgactgc taaaacaaaa ccggttttgg attatattag cgcacatggt cgttatcgtc
    71821 atgggtttac ccggttccgg taaaagttat tttgcttcaa agctggccgg aacgcttgga
    71881 ggggaataca tcagttcgga cgaagtccgg atgaagaggc tgtccaggag aacctacagc
    71941 gaggaagaaa aagaaatggt ttaccgggtc atgggtcaga tgatgtccga agccctgtcc
    72001 cttaaaaaac cggtggtcct ggacgccacc ttttacaggg aatcgttgag aaattatttt
    72061 gaagaaaaag ccgctgcatt cggagaaaaa ataatgttta tcgaagtcac cgccccggag
    72121 cacctgattg aagagcgcct gcaaaaaccc aggacgatga gcgaagcgga ccaccccgtc
    72181 tacctgaaac tgaaagcggc ctacgaaccg ctgaaacggg aacatctcac cctggtctcc
    72241 tccaaccata atatttcgga gatgctctcg gaggcattgg cttaccttca ctccgtccaa
    72301 tgaaccggga acaaattgat atccttttaa aggccgggag gtttcctcac cccagtctga
    72361 tcgaaaccca tatttcctac gttatccttg gaaattccct ggcatacaaa ttcaaaaagg
    72421 aaatcaaata ttctttcctg gatttttcaa ccttaaaaaa acgcaagttt tattgcgagc
    72481 gggaagtttt attgaacaac cggttttcga aaggggtgta cctcggcgtg gtcgcggtgc
    72541 gccaggataa aaagggaata accgttgatg gcccggaagg taaaatcatc gattacgccg
    72601 ttaaaatgaa acggctgaaa tcctcgaacc aaatgcaccg gatgcttgaa aaaaaacagg
    72661 tgacccgggc tcatattcag gcactggctt ccacgatccg gcgctttcac cttcacaccg
    72721 atgtgatccg cgcgcctttc cgcccgcgca cgttttcatc caggtttaat gacctgcttt
    72781 cggtcagcgg ttttgtaaag tcggtgttgg gctcaagctg ggacgaactc attaaaaatg
    72841 cggtccgcct ctccgaccgg ttcctgaaac tccatcatga tctttttata aaacggatta
    72901 aaatggggtt cataagggac tgtcatggcg acctgcattc ccggaatatc tttttatacc
    72961 gcaagcccat cctttttgat tgtattgaat tcaacgacga tttccggaaa atggatgtgc
    73021 tggatgagat cgcctttttt tgcatggacc tggaagcgga aggattctac ctgttgagca
    73081 aggccttcac ggacgattat tttcacaagg accccggcgc tttcggcccc cgggaacaaa
    73141 tgcttttcac ctactataaa agctaccgag ccaatgtaag ggccaaagtg aatgccctga
    73201 gggccatgaa ggcagaaggc gctcagcgta aaaaatacct ggatgaagta aaaaaatacc
    73261 tgaaattgat ggatggttac ctggaaacgc cgataccaaa atttcaataa cttgtattct
    73321 tatgcgcctg caatatttgc ttcggtgggg tacctgcctc atgcttaccg cactggggac
    73381 cggggcaaag ccaggcgggc ccgcgaattc caaaaaaatt gaaatacggg ggatttacgg
    73441 gagcccggag cctttctgga aaaaggatct tcacctggac caactcggag taaatgcggt
    73501 atttatgcac gacggggcga tcaatgaaaa gatcatgtcc cgcgccagat tagaggggct
    73561 taaggttttt gccgagtttg ccacactgaa tggcaaaaat tatgtggatc agcatcccga
    73621 agcctgggcc atcgaccaac acggcaagaa agttgaggcg gccacctggt ttatgggcgt
    73681 ctgtcccaca gaacccggct tcaggcgata ccggaccgat gcgctgaaaa acctgttgcg
    73741 aaaatttgac ctcgacggag tctggatgga ttacctgcac tatcatgccc aatttgaaga
    73801 tccggagccc atccttcctg aaacctgttt ttgtgaccat tgcctctctt tatttcaggc
    73861 ggctacagga ttgccattgc cggggggaaa taccgctgaa aaagcggatt ggatcctccg
    73921 ccatcaggaa caaatctggc gaaattggcg atgcaccata attgccggct gggtgcggga
    73981 ttttaagaag atcctgaagg aagagaagcc cggggccctc ctgggcatct atcattgccc
    74041 ctggaatgac gaggatttta acggagcccg gcgccggatc ctgggactgg attatgacct
    74101 gctccgcgaa catgccgatg tgttttcgcc gatggtctat cacggccgaa tgggtaaaga
    74161 tcccgaatgg gtcaaagaaa acatcagctg gtttacccac cggttggaca tccagccgga
    74221 ccagtaccca aaagtctggc ccatagtcca ggcgcacaac gatcccgggg tcatcacgcc
    74281 agaggtcttt gaaaaggttt taaggtatgg ggccataggc cggtccagcg gggtgatgat
    74341 gtttaccagt tattccgtgg ctgaagatga ggagaagacg gcaatcatga agaaagttta
    74401 tacgggctgg taagtccggc tgtaaaaata atcatcaccg acctggtcgc atttataagg
    74461 ccatttttgt accggcttcc ggcaacctat tgaagtcctt attaccgccc ttttccctat
    74521 ttaagtaaca aaatcccggc tctttacact gtttattaaa tcaataaccg atatatgaat
    74581 ttcagaatgt ctttaaccag attttggtgc ggtctgctta ttccggccgc cttcctgttg
    74641 gtgagcagtt gcaaatcaac aaccgtctta ctggcaaact tcaataatga ccatatcgga
    74701 tcacctccgg cccccaacca agccaccgga accgtatctg tcacatccgg cggagggtcc
    74761 gtaatcgtga cttcacccaa tgataccata aaatcaaaaa aatggtgcgg tataagtcat
    74821 ccatttaata attcagatcc tactgaatta accggaaact ttacccagtt tgggaaaggt
    74881 aaatacggtc ttttagccag cctgtatata ccggttacag aggtagtcac ggttcagttc
    74941 gaagcttcca agcaaagtcc tttgccattt ggcccatttc tccatctcga ttttatgcct
    75001 gagggtgacg tgcgtattga cgatggcacc caccgttttg gacagtttcc gaggaatcag
    75061 aatttcgtat tatcagtaaa tctgaatata accgatgtgg ccgccacggc ggagattact
    75121 ctcctaggag ccaatgcttc cgggaatacc acggtgcaaa tttcccaaaa cgtccttcct
    75181 ctggcccgtc attttggcgc tgtcaaattc tgggtcggat ttcaacaccg ggctactttt
    75241 tttgtggatg atatcattgt gaccaggaaa aatcaataat tttaatccgt taattatttt
    75301 aattccttaa ttacttcttc accgcccact tcaaacacac cggtttattc accgacaaac
    75361 gtttgccggc atcggtgagc aggcccgtct cccggttgat cttgaaaacc acgacatcgt
    75421 tggtgttttg attggcggcc agcaggtaat tgccggtcgg atcgatggta aaattgcgcg
    75481 gggtcttgcc cagtgtgctt tgatggccca ccaggctcag ggaaccgtcc acgggattga
    75541 ctttaaaaat gccgatggta ttggattcca tccggttgga gcaataaacg aatttgccgt
    75601 cgggggatac gtggatatcg gctgagccgt aaggcccggg gtaatccgcg ggatgtccgt
    75661 tgaccgtacc gatcactttc agttttccct ttttgtaatt catcgcggtg acctttcccg
    75721 acagttcttc cataaggtaa gcccatttgc cgttgggatg aaaatcgagg tggcggggac
    75781 ccgcgccggc ttgcagctgc accgatcccg gttttttgga gatgatgttg ccatccggct
    75841 gaagtttata gatcatgacc ttgtcgatgc ccaggtcggg aaccatgagg tatttgtagt
    75901 cgggtgaata cacggcagca tgcacatgcg gagcgtcctg cctgcccttg acgatgctgc
    75961 tgccggaatg ctggatattg gtgggattgc caatggagcc atcggaggcc acaggataat
    76021 agaccaggtt gccggaagaa taattgccgg cggccaccca ttttccgttt ttatgagtgg
    76081 atacataaca aggatggttg cccgcagaag attgtgaatt tatgagttcg aggttgccct
    76141 ggcggtcgtc aaaccggaag gcggaaacgc ccccaccgtt cgggttgccg gtttcggtga
    76201 ctgcataaag atatttctgg tccggagaaa gggccaggta ggaggggttt tgggctgcga
    76261 ctttgtggag gtattgaaac tcgcatttgg cgctgttgta ccggtagacc tcgatgccgt
    76321 cggaaggagc cgtggtataa gtacctataa tcaggaaata atcatcctgg gcgattaacg
    76381 gattcatgaa ccgggtgacc aacaaaggca tcaataaact gaagtacttc atataaactg
    76441 gattaaattt cggaatcaga attccaggtc aaagttaaga accaggggcc actacccctt
    76501 tatttcttta atggaaagcc tttctcctct atagaaaatt tgtcgtggag ggcccggcaa
    76561 ttttgaccca tctcctgcgt tattaagaaa ccggtcaata tgaaccccct gatcgtatgg
    76621 gtaattgcat gaaacgatac ttcctttatt tcttcttttc tttttcctgg tatgtcctgc
    76681 ctgcccaaat caggtttacc caggtcaaaa ccttatcggg caccctcacc gatatcggcc
    76741 atgcggggga tggttccaac cgggtgttca tcacccataa aaccggcacc atcaccattc
    76801 tggaccaggc attcaatacc ctgggtacct tattggatct atccggacaa ctggccaccg
    76861 acagcgaaaa gggtttgctt ggcctggctt ttcatccgga ttttgagaac aacggccact
    76921 tctttgtcaa ctataacccc gccaatacca atcatacggt cattgcccga tttacggcaa
    76981 gcacaccctc ggccaatact accgtgagta tcggcacact caaaatcatt ctaacgatca
    77041 ccgaggccgc caatgccaat cataaggggg gcgacttggc ctttgggctt gacggatacc
    77101 tctatatcgc taccggggac ggcggaggcg ggggtgatcc gcaggggtcg gggcaaaacg
    77161 gcaatatcct gcttggtaaa atcctgaggc ttgatatcga taccgatcaa gcttacctgg
    77221 tgccgccttc caatcctttc gtatccgatc cagggatccg cgatgagatc tgggccctgg
    77281 gcctgcgcaa cccctggcgg atcagttttg accgccagac ccaggatttc tggattgccg
    77341 atgtgggaca gggtgcacgt gaagaaatca atttcgaatc gggcggtttt gccgggggaa
    77401 ggaattatgg atggaattgc cgggaaggat tcctttccta tgcaggctgt accggtaact
    77461 tcacccaacc gatatttgat tacggacact gcaacccctg cagcaataca gccggtacgg
    77521 gaaattcaat taccggaggt tttgtttacc gcgggacaaa acccgccaac acccccatga
    77581 ggggctatta tatctgtggg gattacgtct cgaggcatgg atggatgatc aaatacaacc
    77641 ccgggtctcc ggtcgaatcc aagaccatca gccgcctgac gccgggtggg gtcaccacct
    77701 tcggggaaat ggaaaacgga gaaatcctgg ccggccagag caatggggtc ctcggattga
    77761 tcgaatccac ggaaacggct ctgcccttta aactgagccg gttccaggct cattggatcc
    77821 atcccagggt ggagctccga tggtccaatc atacggaaac ggatcttgcg cactacactg
    77881 ttgaaaaaag caaggagggt ctccggtttt tatccattgc ccaggtgatc cccaaaaagg
    77941 aggaaagcgc tcccaacgat tatatctatt acgatgaaca aatagaaagt ggtgaatcct
    78001 attaccgttt aaaactgacg ggcacggatc agtcggcgga ctattcagac atcgtccggg
    78061 tccgggtgcc ggataacgaa aattcactgg ccttctaccg ggcttcgacc cgtga
//
LOCUS       DAOQXT010000004        77937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_319_length_77937_cov_6.048021, whole genome shotgun sequence.
ACCESSION   DAOQXT010000004 DAOQXT010000000
VERSION     DAOQXT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 77937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77937
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_319_length_77937_cov_6.048021"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2036
                     /locus_tag="PKM27_00915"
     CDS             <1..2036
                     /locus_tag="PKM27_00915"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR05840.1"
                     /translation="EAESLDMSFIDEYAEDTGSSCRVIHRIWTITDRCTYQPSAPHGP
                     DVILHDSMVADPVDRYCVYRALKDNGDGYMRYTQQIRFVDRTPPRLSFRDTVIYTGAA
                     CVSEGFSLFPTVSDDCTGTASIRIQAWLDLGMDNTLDQEFPPGIPLDIPGGFGPGIFG
                     LHIIAEDLCSNRDTCIIKIEIKEGGVPSAFCLPGTAIIDIPASGQVTIYARDLDAGSL
                     AGCTPGPLRFSFSPDPADSIRSYTCDSAGTKTLPIWLTNEGGFQAQCETQIRLLPGPD
                     ACIPSAEIRVKGRVFTGLKIGIKQVNIGFEPLETDTLTDAEGYYSSSPLPAGLSLTVR
                     PSKTDGPLNGVSTMDLLLMEKHILGQSLITDPLSLIAADVNRSGSVTVSDLIELRRLI
                     LGSINTFVNNTSWRFMPAGHLFTNPAKPWLFPETIEFKNLMGPITNADFIGVKTGDVN
                     YTVGAGLSGLESRDRDRVDLKVETTEEGGELKIRIFLAEPLTQVDALQLGIALPGSLQ
                     SIECSGGQLSIRPDQYNLLHGNQIRIAWVNGQGKNLNETEPLLEFTGKKGGALLQTQL
                     IDSGIFESFLSISGKPRTLYCQAVKAAGIQLRAFPNPSSDRVNIEFYLPEGGQNEIEI
                     FTMNGKKIYMSESTLYPGLNRILLRKSDLGGPGIYYFKIRTRHTFENLTFILF"
     gene            2684..7780
                     /locus_tag="PKM27_00920"
     CDS             2684..7780
                     /locus_tag="PKM27_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR05841.1"
                     /translation="MPADTCYKVIEPDDLLENPLEGCIYQVALAHPFGTARPPFIAPD
                     AVNRSHIGYTFIYSVYVLDENSNRVNSCWGYVTIEDKAPPQPMCKDATISCFQVEYLN
                     RLVNTVVDNCAQSGKSVITSMTFSDWGCDSAAYIGQVVRTIVSWDTWGNSGTCNDTLT
                     IRKDSLELVKCPDLIELECEDCNPAIVFSDDKDDPNYPDPDFLLELQEDGCLSDTLMI
                     VPMIRDSVLVVQGDTCYYVDSCVNMWPVLGGFCKMTVGYTDQVLTICPDGSGFKIRRE
                     WRIVDWCRNGEDTVCVQYIKVVDDEGPRFTGDPEDLFHYAFASQHDCQANVTIPGLEY
                     EDCSDGVIQTYVIEYLDESHPGKVSVLTGTLPATIKLPASAATLLPPGEGEGDEIFAI
                     GGLKCFYGDVTLQDPCYNASHVPIFICVIDNTPPTPVCDEYTQTTVDPATCWARVYAH
                     DLDNGSRDNCCNTLHFAVATMADIDTATAHLKADLEACGSSYVWHKDNKAAHEWLINQ
                     YINCYVFKDYVDLTQCGNNQVVLRVYEACGIPRYDPHVFPCSPHEWFCYNAYTTDRMW
                     RNYLKLHVDGQKLDPCSAPGQLYCKKDYFKWLINVADSDGDGVSSFIQLIIPAYDGAT
                     ILPMDILALLSIPSICTPFLFDDQIAFGLRAALPQNASDFSGAPGNTCSQLRYNDCMV
                     NILVDDKQPAVCEDPDDIFWFCDNVSDQVTVNGHRRYYNYWRSDRVEYADKVCYSNGG
                     DTPWLDGTAEHKGSKDYDPTENAEDGSCYYYPEKSVYNEIECVEEFDDDLTDTADPTG
                     KAYGWYGCNIYGGYHDGKDNCISSYSRQLGLEGTAAGRNGGYNSWAPVYCHTWLCLDR
                     KDSGGKIDPKSAFFEPVFDSNADGELLEPGEGEFLVWDNCSTPELVSSVDDGFIDNCG
                     NGWIRRTWTFSTGCENETIVCDQKIITKHRSDFEVVFPADLIVTCEEDDLGSDGKGGP
                     SPDATGRPMIMDDDCELVGVNYSDERFDVIPDACYKIVRTWKLIDWCKFNPNQHERDP
                     EVIVDDRLVADPLNRYCVYRNLKDDGDGFMTYTQIIKVIDETAPEAGVIADTTFCVYT
                     GYDGAGAEDFSDCTPGTYTSPNFSANDQCTPSAEIRFRWLLDLDSDGSIDDFSGVGVK
                     YFTHSELTPGTHTVSVIAEDRCGNADTSYFEITVKDCKRPTPYCYNGIATVIMPSSGS
                     VTIWASDLDAGSFDNCTAHDHLIFSFDEAGLQPSLTLTCDSIPNGVSFTKVIRIWVTD
                     EEGNKDYCETFIVLQDNAGNRCPDVAGVNGTIAGKVATESTEPVEHVILEAKSAGQNL
                     PVFKTDVKGTYSFSGLPMNKNYSIIPGRNDDPMNGVSTIDLVLIQKHILGVLPLNSAY
                     KVIASDIDKSNDITAVDLVELRKLILTVYDKLPNNTSWRFVPKSHTFNNINNPWGFPE
                     KIDISNLTKDELNRDFVGIKVGDVNGNVVPHSLLGGEARDNGSSLKFRTEDRLVKAGE
                     ETVIAFTADNFRNIEGYQFSLSLKGLELKGVKNGLLNLNENNFGLTRLGEGYITTSWN
                     ESNGISAGSNEILFSITVKATKAVQLSESLVINSRFTRAEAYDGVKSMGVSLEVGHKA
                     GSGYALHQNTPNPFKSATVIAYELPKAEKVTLKLTDVTGKIIRVYQREGVKGYNQLKV
                     NRNEVGGAGVLYYTIETKDFGATRKMILVD"
     gene            8058..10178
                     /locus_tag="PKM27_00925"
     CDS             8058..10178
                     /locus_tag="PKM27_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR05842.1"
                     /translation="MNFLRSLIFFLLCSSSLMAGNPKFTISSEAGNTNDEVTIKFTVS
                     DFIDIAGIQWTLRWDPTCLQYVSIQDFNLPELNINSFNVSKTSQGQINTVWYYQQGAS
                     KNNGDVIFTIKFKLIGADGRSCGVEFSNNPLPIEISDKNAMKCTETTTNGKVDIGNVT
                     TPTGFVISAGNTQAAKDQEVCVPVRVSGFKSVNAMQFSMGWDTSKIKFTKIQNFSGLT
                     SFDQSSFNLALVNAGKLVCVWFDQNTTGQTVTDNTAIFEICFRYTGVCPGSGTVNFTD
                     DPTKTVVNSTTGALTVTKTSGTITASNCSSALAVSVTKTTHPCPGQNNGGIEISATGG
                     SGAITYTWTNNSTSQNLSGVGAGSYNVTVKDGTGATATLNAAVNLAALAVTSSKTDPA
                     QGQSNGVITLTVTGGNTNYTYKWSNNATTKDINNLAAGSYSYTVTDANNCTVTGTVAL
                     GPGSTVLDIASVTPTNISCKGDATGAIDIAISGGTSPFTYAWTGPGNFSANTEDLTGL
                     KAGTYKVTVKDGANATKTSNDIIITEPATALKITARVKPVSIAGNDGEVITTVTGGTP
                     AYQYRWNNGSTNSSLTSLAQGSYSITVTDSRGCTANATFNVNPSAGSECFTSQRVFTP
                     NGDGINELFVINCAETLSNQLLVFNRWNQLVFKADNYANNWLGTDQQGINLPDGVYFW
                     VLKDASGVTSAVYKGHVTLLRTLN"
     gene            10219..11139
                     /locus_tag="PKM27_00930"
     CDS             10219..11139
                     /locus_tag="PKM27_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNR05843.1"
                     /translation="MKKILFLAVCFWSANLGLKAQEEAIYSQYLFHPILVNPAYAGFL
                     PDHELLFNFRNTHAGFPGSPQTYTLAFNGPIAPKLGFVGLLNNDKAGDLNRFRGQAGL
                     AYMFKMGTVKMNAGLAAQYQKFQLANGAILDSGIDPSDPLIRAAADGLNFFNTSIGVY
                     GENDGKLFFGLSVVDLVRTRIDDIQSPTDESSFFKYYNAWVGYRWNIANYNFTVEPSV
                     MIKRLRNVPFQTDFNVRLSFLDDQLFGGLTYTAGGFSKTSFLLGTRINTFRLYYSYDV
                     ALEDFQKYNNGSHELSLSFRIPSVVGKNNQ"
     gene            11386..13710
                     /locus_tag="PKM27_00935"
     CDS             11386..13710
                     /locus_tag="PKM27_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR05844.1"
                     /translation="MKKLMVFFIGVAMFCIPSLAKASLEIYCPNDKEITYAAYQAGKY
                     YDKPVVHGGYYTLYGPWIYKHLNCGSGYVEVRWEILDHYGRKYYCNQTIWVTNPSSPA
                     PISIWCPKEKVVYCDQVDTMQYPQPIVTGGSYWLYGPWVSKSLNECGVGSIWVEWKIV
                     DGCGKAYFCKIGVWVKPRNTYAKIWWPKDFEADLCTDQTDPKYLPWPYGYPKVDQYNP
                     CAKYGISYKDEEYTFPNDTGICKKIVRWWTVIDWCTYNPNSYGYYTPGKWTHAQLIKI
                     VSRSKPDISCVPDITVGQETYAQSAWVDIPVPQVTSSCSGKTVISHNSIYATSPGADA
                     SGIYPLGKTKVIFTVRDACGNTDTCSTTVTVLDMTAPTPYCIGSLITSIAWHADGVYT
                     ILDPKKFDVGSFDNLTPKHKLKFEAVPNRFTCDSLGARQVRIWVTDEAGNKDYCTVTL
                     ILQDNLGMCPPPVLPEDTLFIEGSLLSTGDAPIDSVTITVYSDDTTGSLALDGTYRAE
                     GLTAGSNYIITHSKSDQFLKGVSASDYKLLTDHIQGYDTLDHPYQLLAADINGDDTIN
                     VEDAYLLGYYLLSGSQVPPSQASWRFVPSDYDLSLIQVLSNSIAQVPRHRIFNPLNAS
                     VSGADFTGVKIGDLDFSLQEEISALEFQSGSPAKAEFVNPGYWVKKQRINEAEASGKR
                     AATLVTVKESTSLFPNPFNQFTSLQFSSPADGQGILRIFDLNGKLIYSRLESVSPGVN
                     LVRIEGSNLRQKGIYIYNLTLPGMNLNGKLTFID"
     gene            14098..17694
                     /locus_tag="PKM27_00940"
     CDS             14098..17694
                     /locus_tag="PKM27_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR05845.1"
                     /translation="MNPIMNLGKGIFLVLALLTLPLNTIHSQCFLENGTYYTAPCDTN
                     LADFLHPDTSDIGNCPNIVSVEANLPFQTIMNGCDSACFSLLIRQWRVNFSSGPDTLL
                     VDTICFTPISLAEVFCPRDTVIYCNGGLADTSVAALGSPLHNNGIPCNILVNGPTQIL
                     WSSTSLGCLKFTRQWLVMDWCTLEIDTCRQTIEVRDTTDPSISVIPLLQDFSTSGDAC
                     TGSVIFPVATVSDNCAAPGQISVKIEVAGQTLNSNGGIVHNVPQGTHQAVYIATDGCG
                     NSSTDTTVVSVIDKVGPIAICKSAKSIQLSQTGILVMPATAFDNGSFDLCDPEITVKV
                     KRIPSSSFCAMPYNQVHKFDDMVKFCCMDANKDIMVIVRVYQGFIPAGPISIDAFSNP
                     YTECMVMVRVLDKVGPVISCPADTTIDCRDIHKKRVRSLTDYGTPVVSDLCLDTVWID
                     SLPQLGVCNTGVIIRRINARDDAGNVSTCSQKIFVINTDPFDGKDTADIAWPKDTTFF
                     VCSANTSPANTGFPVIKDASCSQIVFGSQDEVFQYVPNACRKILRRWTATDWCQLSPT
                     DSKAGKWYYTQKIIVMDTLKPMITVPDTVTVNSMDSICGAVQVSVPLPSATDCTDSAS
                     LIWSFITDINGDGIHDGQGYGKDASQTLPVGKHKIIFSVNDHCGNTCSDTTVVIVRDA
                     KAPTPLVMHGLATDLSLMNGVVMARVPASLFFVPGSAVDNCTPSDKLIFSFSPDTSDR
                     FRIFTCDSLGMRTVYLYVTDEAGNQGEVISYILIQDNFGSCPGAASGIKTTGVGGKIQ
                     TEWGSHVEQVDIKLMHGQTVMQGVNMNQGQYKANGLQIGQSYEIIPKKNVNPYNGVST
                     LDLILIQKHILGLQPIRSPYKQIAADIDKNGEINGIDLVELRKLILGIDKAFTKNEAW
                     RFIDAGYRFQEKADPLKQPFREQYLIRDLQSPMQVDFIGVKIGDINESSIPSELLNLE
                     SREATPALAFHAIDQTYSSGDLVRLEFFGAEQTSINGFQLSLHAADLDLLSVEPGLIP
                     AGPEYVHLEGHTLKMSYHQSNALRPTVGMPLFTLVFRARNDGRLSRAVNLGSEPWLHA
                     EVYDGALKASPLKLDWKGKANQSFNMQLGQNRPNPFFESTVIEYQLPESSPVTLKVMD
                     LRGRTLLLRQESGTRGHNSITLSRKDLGTGGMYYYTVESVYGTLTRKMILAD"
     gene            17728..20931
                     /locus_tag="PKM27_00945"
     CDS             17728..20931
                     /locus_tag="PKM27_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR05846.1"
                     /translation="MKIIKANLPIPKTKVSLIQIRRVLSLLLFLSPLLVRSMVLHQGN
                     TDHSNYYTQSPWQPVYLLEASMAGMTCYTQLNLSLGAGGKAKILPSMILKPGPINDAD
                     FMVRIYGTSTDTLSCLHKGQKVKVEVTEIATGNKCWTFILVEDKMAPSIQCRDTSFLC
                     VGDLSYLDSIPGLYEVGDNCTPLQKLKITHIDKVEMLECSSDTFSVVRRTWTVVDPYG
                     WSGSCTHNIALLRPQKSDIDFPQDTTLYCPDTDASPSNTGQPTIGGQPLDKFCGWSFR
                     YDDVVFNKCGQTKKIWRTWSVLDCCALEDTTVTQWILISDTTDPVIQCAPVDSVSTLA
                     ESCEAKYIIPEILSATDSCHSSLTTIVWVDSQFIAVPGSMVLLEIGTHSLEYQVSDPC
                     GNTYSCMTSIVVEDTHAPVFWCTDTIQIGLPADRVMIGASFSEIEAVDNCSLVGVQIR
                     RKTDFCMDGSDDTVFGDSVAICCSDVNNPFDIVFIATDIYGNVDSCVVTAVVVDKSPP
                     VLTCEQDTTILCGEIVPDWKDPLAGLTDNCMDSIAITIDTLINDVNLCGVGQTVRQII
                     ATDLAGNRDTCIQRIAVSAGDTLTPDDIDLGTDTVRVMGCNLALLLPDSIGYVPPVVD
                     TPLNDCHRIFITYIDSVQPLGGTSLCRITHRSWRVGDSCYSVFPVEVLVQVILQDTTA
                     PAPLRGGITGRVTNIAAIPIEKVHIEGKNMQAQPVFEQMTGVDGQFSSGRPDPALKIR
                     LEKEDPAALNGISTLDLIKIQKHILGERKLEHPLLEYAADVDRNGQVNVLDLLTLRKT
                     IIGLSNPDLPWHFVIQSAPSARIQPGSWLPEEYMLFSKPSVEEFIGFKTGDVNMDAVV
                     NHTYQLEGRSAAVTELILEDSEEGLLIKAAKDMRMLGMQLSLVSEDGWAGTHLVSGIN
                     KPLEGHWSVIRNELKISWNENQARVLKAGTVLFIIRGAHSKNVFSGTLPSEWYDHQEK
                     ALPVRLVKSDKKTDPVRISAFPNPVSQQLNIRFPGQGPIQSWRLVNQTGRISLEGKSP
                     GHNEWSINMQILPSGLYWLELILSNGQIERHKVLKIN"
     gene            21041..25417
                     /locus_tag="PKM27_00950"
     CDS             21041..25417
                     /locus_tag="PKM27_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05847.1"
                     /translation="MKTTSFLFRRSAWVFALLVQLAVDSAFSNTLFAPARYHYYQINP
                     FEASEALGGNCHKDKQPPVAQLKMLGETECTSLQGAPWSDFLILSDNCIPNEQLYVTY
                     KDSTYLNCEEQVILVFRKWKIKDLAGNTTVIDQMIPVQKIPLKQIELPLDVKVYCPEL
                     PDAAALGEAVPTYKGKPVSHFCGYTVDYKDKAAVLCGNSTVITRHWTITDCCNSYAID
                     HEQQIYYLDTTRPVLTCPAPMVFKTNVKECYSHQRIPGIAATDGCSPLGIKIQVVVDN
                     GKTYKVGDLVILSAGNHTFEYKVTDACGNMSTCTVPVTVVDGQGPLLSCKAIEVCLIT
                     DSIRVGPEQLVDEYWDDCQGLKKVSLKIRKFVDLCGDPKDDLVFKDSVTICCAGGEDV
                     VRVEIQATDAAGNVSYCRSDIYVTSKLNVDIECLDTVEVDCGEPLPEIIPEVIACGDY
                     DLDTKIIFDNRDAFGIGTVIIRYIVTTAGGMQDSCQTVFEVGLEGNPFGPEDVICPPG
                     TVTIAGCTFPDIAGIPGISLTDTARPCSQVNVNLQLDTFTDLGIPCLRITRTWTVTDV
                     LQPGLQVICVQNIDIIDTIAPVLSGARDTTVVAGLNCTAIVDLPEVIATDCDTGIVVT
                     NTFNAQGGNLDSIVFPLGQTRITFWGVDACGNRDSLSILITVIDTSGFGIVCQNDTIV
                     NCGTVFVPRPAVIEASCTQVATNVLTSDTVSNKCDITHIHFKRVVTDTLGRKDSCTFT
                     VTFRASDTLFCNQIQWPKDTLLASCGKSIHPDSLNLKPIFTFNAGSCARVVVTYKDTS
                     LAGGGGTCTNITRRIWTVSDTCAIPPVVCRDTQLISVVDTSRPILQVPKDTCVFLAFP
                     ALCDTLLGFLGNATAQDCDPAVIIKSVVIGTTDTGGASLVRRYGLGDTRVLVIAQDAC
                     GNRALDTVLIQVKDTVRPDAVCVKSNNYFNDQGLVRVNARTFNGGSTDNCTPPAMLRF
                     SWTKNLADTILEVNCDVIKIIRASGDTILDSVIVMPFERNFNLWVTDASGNQDTCVGN
                     RFLAFFDTLNLCGKNAIKNLSSITGKISMENGLAVPKVILSAIGDEQYQHLSDEQGTY
                     MFDKINPGIYKMFPYKNDDTRSGISTADLIAIQRHILGEKILNPTQQIAADINKDEVV
                     TTLDLIELRKVIVGIYEVFPQNTSWRFFDQSLMEKMDDHSVMKEMENPFVEAAEKETS
                     QLNFMAVKIGDVNGSTVPQFAALESREQSTRWLNIPAWNLNEGEQVELPLSLNLDDLV
                     ALQASIFFEDLDILGIKEEPGALIKPHFVATNQLGKGYFNFSWIKSGPAFPAQATHLV
                     SLVLRARRDLALSEKVTLVHKSLAPEAYHQGGSVSALQLRFEPMQTGKILDRGIVLNQ
                     NIPNPFTGSTRIRFSLPEAGETKWTFMDITGRVIRSWSLPFDRGSHEMVVDKAGLGTS
                     GVLYYRLDFKGYTETRKMILLD"
     gene            25987..30336
                     /locus_tag="PKM27_00955"
     CDS             25987..30336
                     /locus_tag="PKM27_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR05848.1"
                     /translation="MLIFLFGWSASQQSAIAQGCALTCHNINLSLDTVQGGVSVILPE
                     HLLSGEASCPGGGFKVTVYAPNGALIGNSVDCSWVGYSLIGKVTDTISGNSCWAKINV
                     EDKAGPNLNCVRDTIHCLEVDEYSLGADRFPFGLAIDNCGIKSQVSISMVCTEYPCSN
                     TQFVGLCIRTVIATDKWGLTSSCQDSLWVSRDSLDNLMGVRDTVLDCAIADTMAKDAN
                     GNPIPQRVGTPTISGIPVWPNNNVCKILSKYTDVVYPICGSKSRKIRREWTIVDWCAG
                     EDTVLIQWIKIVDTTKPVLVDRGEMSAYVGPHNCQAPVDLTSPGATDCSGIKEVFYSA
                     VVLSDGHGGLGAVYTGYINGGSKRIYLPSGRHEVHFFVTDHCGNTALDTLIINVTDHV
                     PPTPVCDEHTATTLDPVSCWARIYASDLDNGSRDNCCGTLYFAVAHMDSITAYTELLR
                     GKIISKYKESVYHSNRDFFEQVIDRWINCHVFEEYIDLGYCGVNQLVLRVYEACLIPK
                     YDPHVHSDSRQSFYCKEAYNGHDGAYNQAQDNVDALIDYVLKGIGMKVQPNFPLGYRN
                     YSDCMIFVNVADKVAPTCEVDAEKKVYCDGVPYVELAWNYGGGKDLLNCYGANGIYSP
                     DYLELDQYDNGGKINPYEYFDNPVFEDNCGTVKVDTTTTGSINNCGAGVLTRSWTGTD
                     ECGLLSTTCAQKLKVLHRSDFEVLFPKDIETVCLDNPAQFNNPTGENYPKIFDDDCEH
                     IGITFSDQRFDIVADACYKIIRTWKVIDWCAYDPDQHYRTKDYIVDTATEYRAVPDKY
                     QDRFCQFRYLKDNNDGYILYTQIIKVKNTVAPTIAVEDSVVTCAGSVETCLGHIKLKV
                     NTTDDCTPGPEMRWVVYIDRGNDGSTEQTSYHSGSTVTVDSDFEVGQHKISFLVEDIC
                     GNETRKDFVIDVELCKLPTPYLLNGLAVDLMPIDNNNDGNTESGMVRIWASDFDAGST
                     AGCGQQVVAFSFSSDTSDKSRTYTCDSIGQRFVNIWVTDSYGNQDFASTYILIQNNAN
                     ACAPGTQAITGTITGRIKTEDQLDVEKVSVQLQGANSLPAVTKADGLYAFSSMKMGGS
                     YKVMPKKDINPMNGVSTLDLLLIQKHILGLQPLNSPYKLIAADINKSNEISSVDLVEL
                     RKLILGIYDKFQGNESWRFIPKSHTFKNPANPFESGFPEYQDISSLGHNSFADFVGVK
                     VGDINGTVVANQLLGSESRAPGKEFLLRADDLSFNEREQILVPVYAADLQLIQGYQFT
                     LSFDPKVLKLSQVIPGAKGMSQVNFGLQRAVEGRITTSWTTTRALESMDQPLFTLAFN
                     TLRKGRLHDQLYINSSVTVAEAYDSQHDILNVRLQVGSKDGKGEFALLQNTPNPFLHS
                     TRIGYQMPESGQGLMKIFDLNGRLVKEINLKYQKGYNEIRMSRNELKSSGVLYYQLQA
                     GNFTATKKMVVIE"
     gene            30471..30878
                     /gene="ruvX"
                     /locus_tag="PKM27_00960"
     CDS             30471..30878
                     /gene="ruvX"
                     /locus_tag="PKM27_00960"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNR05849.1"
                     /translation="MARALGIDFGFKRTGLAVTDPLRICVNRLETIPTETFDQFLEDY
                     LKREEVDLIVFGEPLHRDGTATAVSMEVHEKAKKLKIKYPSLKIDFQDEEGTSRKAVR
                     MLIDKGTPKSKRNKTSIDQMSAVLILQEYLKHY"
     gene            30880..31443
                     /gene="def"
                     /locus_tag="PKM27_00965"
     CDS             30880..31443
                     /gene="def"
                     /locus_tag="PKM27_00965"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNR05850.1"
                     /translation="MILPIYAYGQPVLKQTGEELDFDRFTGLEELISNMWDTMYAAKG
                     VGLAAPQVGLSLKLFVVDTTPYEEEGKPFTGIKKVFINPVKLDEHGEAWSFEEGCLSI
                     PDLRAEVDRPERVNLSYFDETGTVREELFDGLNARVIQHEYDHIQGILFIDLLKPLKK
                     RMLQRRLEAIKKGQVEADYKLRFYRKP"
     gene            complement(31428..32033)
                     /locus_tag="PKM27_00970"
     CDS             complement(31428..32033)
                     /locus_tag="PKM27_00970"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNR05851.1"
                     /translation="MFTGIIEASGKIIGLRPDKSNIQVFVESPISNELKIDQSVAHDG
                     VCLTVDELGPGWHRCTLIRETLDKTHFSDARQGEQINLERSIRFDSRMDGHFVQGHTD
                     TTLTCLARSDRRGSWVFEFTLPSPFKSLVIPKGSIALNGVSLTISGLLNDSFEVSIIP
                     YTYEHTNFQFIHAGRRVNAEFDLLGKYIQRSLELRSSQGLR"
     gene            complement(32035..34767)
                     /locus_tag="PKM27_00975"
     CDS             complement(32035..34767)
                     /locus_tag="PKM27_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR05852.1"
                     /translation="MKIFRSISLFLFLTCYAGIASATHNRAGDITYVQIGPLTIRLTV
                     TTYTKASSTGADRDSVTVVWGDGHSQAVYRINGPLGKGEILQNDTKRNFYVAEHTYPG
                     TGTYTVTMTDPNRVGNILNLNYPNSVGIQFHIATTLTLLNSTIQGYNNSAVLLQPPID
                     FGCIDRKFVHNLNAYDPDGDSLAYELIVPFQATGSEVPNYRFPDQIVPGADNKISLDR
                     TTGNFEWIAPQREGEYNIAILIKEYRNGILLNTIIRDMQITIENCSNEPPSLTVPNDL
                     CIIAGSKVEFQVQASDNNLSQKIAIFALGGPFNVSVNKAVFDAPSGFQNQIARGTFSW
                     QTSCEHISDIPFTVVFRAIDNFKDSSGLADLKTVRIKVIGPPPENVSSSTANNNLIRI
                     NWTYPNACAVTVNDYFKGFSVWRKINSSQFLIDSCTPGLAGRGYTRIASNVLSRDQDT
                     YYFEDDMVESGKTHCYRILAEFALTSPGGNSYNRVASLPSEEICVQLKRDLPFITKAT
                     VNTTDPTLGEIEIHWAMPLADDLDTLKNKGPYKLVLLRADGIFNTNTVFTPIPGAEFT
                     SEFLKTFTDTVFLDRNLSTSEKPYSYLIQFFTGNSPTPYGSSFPASTVYAKTIASDRQ
                     VLLSWEAQVPWNNYNFEVHRRSGSGAFELVGSSTTPFFRDKTVSNNSTYCYRIRSIGT
                     YGINGLPSPMFNFSQETCATPLDTVPPCSPDLSINRNCVTKEPDGSIANELVWGFSNT
                     DTCFIDDLKAFNIYFKNNKSAGAFRLIATLTDASATTFKHIPDSGFTGCYVITTVDFN
                     DNESRLTGEVCPANCNLEYELANTFTPNGDGSNDLFVPRINAGVLKIEFQIYNRWGEI
                     LFETEDPQILWNGQDKKGRNLSEGTYYYICRVFGFQENTTQVIEDRKGFIQIVR"
     gene            complement(34912..35781)
                     /gene="rfbD"
                     /locus_tag="PKM27_00980"
     CDS             complement(34912..35781)
                     /gene="rfbD"
                     /locus_tag="PKM27_00980"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNR05853.1"
                     /translation="MYNILITGAGGQLGHALNQLLGSQDAFNPVALSHTRLDITDADS
                     LMDCLDTHRIHLVLNTAAYTSVDLAETSPDRAWLVNAEGPRQVAIACRKKQIPLIHFS
                     TDYVYHNALRRPLLETDPCNPRNVYGASKLAGEQKTLYHHPHTLVIRTSWLYGREGHN
                     FFNTIKRLSSEKPVLEVVNDQIGAPTYVPDMAAATLTILSKVLSEAGNPSWYGIFNFA
                     NEGEASWFDFASGIVSGSSNTCSVQPVSTSVFPRPAARPSYSVFNLNKIKSTFQLSIR
                     DWKTALMDCMGNS"
     gene            complement(35788..36336)
                     /gene="rfbC"
                     /locus_tag="PKM27_00985"
     CDS             complement(35788..36336)
                     /gene="rfbC"
                     /locus_tag="PKM27_00985"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNR05854.1"
                     /translation="MALVVEPCPLDGLLLIRPSIFADHRGYFYESYSDKDYRAAGIEA
                     DFVQDNQSFSKKGVLRGLHFQNPPHAQAKLVRVTSGAVYDVAVDLRPGSLTFGRYFGV
                     ELNDANQLQFFIPRGFAHGFLVLSASAIFQYKCDEYYHPSSESGLRYNDPAVGIEWPD
                     PGVPIEISTKDLRLPLLKDLKT"
     gene            complement(36339..37646)
                     /gene="der"
                     /locus_tag="PKM27_00990"
     CDS             complement(36339..37646)
                     /gene="der"
                     /locus_tag="PKM27_00990"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNR05855.1"
                     /translation="MSNIVAIVGRPNVGKSTLFNRLFGERQAIIDDTSGVTRDRIYGE
                     CEWNGKNFTIIDTGGFVQHSDDVFEAAVRQQVRFAVDEANAIIFMVDVTTGITDLDEQ
                     VANLLRRSNKPVYLAVNKVDNHQRMLMANEFYGLGFDNTYFLSSISGSGVGELLDDVV
                     ELFAPEDKEKEQSQLPQIAIIGQPNVGKSTLLNALLGEERNIVTDIPGTTRDSIHTLY
                     NKFGHQLLLVDTAGIRRKTKVHEDLEFYSVIRAIKSLEECDVCLLMIDARHGIEAQDS
                     NLFRLAIKKHKGILILVNKWDLVEKDTNTLHQYEKALKHKLAPFNDLPILFISAQNKQ
                     RIFQVVESAMDVYERRKQKIKTSDLNEFLAEINAKNPPPAYKGRLVKIKYVTQVPKPY
                     PAFAFFCNNPDYIQNNYRQYLENQLRERYPLSGIPISIFFKEK"
     gene            complement(37654..38547)
                     /gene="era"
                     /locus_tag="PKM27_00995"
     CDS             complement(37654..38547)
                     /gene="era"
                     /locus_tag="PKM27_00995"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNR05856.1"
                     /translation="MEKTHRSGFVHLLGNPNVGKSSLVKAFCGQKLAIISDRPQTTRH
                     RIFIIYNDPGHQVVFSDAPGLIKMPQYMLQERMNLQAHAGLHDADILLFCTTAAEPVD
                     PEIAATLSRAKMPVWLVVNKADLFGKARLAEYVAKWAAVHPFQKSFIVSALTLENIPE
                     LMSSLLEALPEGPPYYPKEDLSDRHERFFVAEIVRENIFRLFHDEIPYSCEVQVQDYK
                     ETTGKEGSPFARIFVYIIVERESQKAILLGQKGQAIKDLGIRARQEIESFLGHGVYLD
                     LQVKVEKNWRNNPQALKKYGY"
     gene            complement(38735..39319)
                     /locus_tag="PKM27_01000"
     CDS             complement(38735..39319)
                     /locus_tag="PKM27_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05857.1"
                     /translation="MQDIEPFFRWRDQYVASEDPHSPFYRRQYNEFEYTHAIYNYYIH
                     PQWDEFDSSTLYLKILFVDYAEQFAIIELIGEWNDAIQNDIMYLKRNVIEKLLRSEIK
                     YFILIMDNVYNFHSSDDCYYEEWQEELSEEGGWIVMLGTLEHVEEEMVQAGLQHYIHL
                     GKNFNAIEWRRLDPVGVFAIVEKTLHSQSRRLRY"
     gene            39408..39911
                     /locus_tag="PKM27_01005"
     CDS             39408..39911
                     /locus_tag="PKM27_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR05858.1"
                     /translation="MTGPESAGKSTLSAFLALQYQDPWVEEYARVFLNQGQGIRDAGT
                     LVSLAHIQIEWENLALKDADRVVFCDTDPLVLLIWAHEKFRKRPPELVALARHHRYDL
                     TLLCKPDLDWEPDPLRENPHDRDKLFDLYLDHVNKFRFNYAIVEGLGEARLKKALATV
                     DPFLKAH"
     regulatory      39923..40026
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            40051..42489
                     /locus_tag="PKM27_01010"
     CDS             40051..42489
                     /locus_tag="PKM27_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNR05859.1"
                     /translation="MSLKIFSLTLVLLFTRPLQGQEIILQVLGPDDPVNGVLAYSHDR
                     SRFAISDREGKMNWKLVAFPDTIHISHLSFLDHTEIIQTAALAGGTLLIRLQPRTINL
                     ATAEIATTWVKPNTHVVQSTLNESQIASRFLVQDIPYIIQQFPSTVTTSDAGNGIGYT
                     NVRIRGLDPTQVNVMVNGVPLNDAESQAVFWVDLPDLIASASALQVQRGIGTSGAGQV
                     AFGSSLLLSTYKTSPVPYAQLESSVGSFATLKNSLQLNSGTLGKAWNFSGRVSRIRSD
                     GYVDRAASSLWSGAFNASYIGTGRSVRFFIFDGFERTYQAWYGLPVQYVGDKEKRTYN
                     PAGTEKPGLPYEDQIDRYRQTHFQLIHQEDQGKRWAFQNTFHFTPGRGFYEEYKSGQS
                     PSDYLLDSGDELDIVRRRNLENHFLGSIHTARFRMGDYEINGGLAWNLYSGRHFGQVI
                     QAGNKPLPDQPVYYDNEATKWSAMTFGKMTWDLHPWSLMADLQYRRIGYSYQPAYDPE
                     QGIKTVHHHFLNPKLGFTLAVGPHAQLFGFSGIARREPNRDDYVDADRTLPRAERLWD
                     HEAGFRWGRRNLQIEQNIYYMMYKDQLIPTGRLNDVGAYIRSNVEKSYRLGSETSVTW
                     NPGKLNVQASLTLSRNRTLNYQAYFDDWDSGTQEKVNYGSQPIAFSPSFIGNLSSEYL
                     FYDKQAGQTRHFLFLDGSVQAVGRQFLDGTGDPSALLPAYRHAQLALRYRLIGDKIPS
                     MDFHLQVNNLFNSRYESNGWIYRFRSPSYNPVPDDPYAGGEGDHRYYLKGLYPQAGRH
                     FMLSCRIRLEGKGG"
     gene            complement(42486..43100)
                     /locus_tag="PKM27_01015"
     CDS             complement(42486..43100)
                     /locus_tag="PKM27_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05860.1"
                     /translation="MKYTCLRVFSILMLVLCLSPSRLEAQIYRRQPQKEQSPEMKSFA
                     DHLWYGGGFTINFSSGLAGLDGNTFGIGLSPMLGYKFNSFLSAGPRFEFLYTTGRFRN
                     FGGPVYKYNGLDYGAGLFGRAKFLNVLFAHTEFSYINRVFPVGFNGNRLETERVGDNQ
                     FLVGLGYTGGGLLKSEIYLLYDLTSDSQSTTLPLVYRFGITYNF"
     gene            complement(43117..43818)
                     /gene="cmk"
                     /locus_tag="PKM27_01020"
     CDS             complement(43117..43818)
                     /gene="cmk"
                     /locus_tag="PKM27_01020"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNR05861.1"
                     /translation="MAVPKKINIAIDGYSACGKSTLAQALARHLKYKYIDSGAMYRAV
                     TLYAIRHKITGDLPASLLPHLDHIQIDCVWSESGNKIYLNGEDVTAAIRMPEIQDLVS
                     PVSTIPQVRRFLSRQQKQMAIHKGVVMDGRDIGTVVLPDAELKIFMTADLEIRTERRF
                     KELLLKGLNLDKEVIKQNLLERDHIDSTRSDSPLKKAPEARVLDNSFLNEKQQFELAL
                     SWVEETLAGKPNFEN"
     gene            complement(43897..44118)
                     /locus_tag="PKM27_01025"
     CDS             complement(43897..44118)
                     /locus_tag="PKM27_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNR05862.1"
                     /translation="MYWTLELAHHLEDAPWPATRDELIDYAIRSGAPLEVIENLQQME
                     DEGEIYDNIEDIWPDYPRKDDFLFNEDEY"
     gene            44232..44720
                     /locus_tag="PKM27_01030"
     CDS             44232..44720
                     /locus_tag="PKM27_01030"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNR05863.1"
                     /translation="MGIGAENQDFNFLTQAIQEALAGVRRKEGGPFGAVIVKDGLIIA
                     AGNNQVLLTMDPTAHAEVVAIRRACQVLGNYQLHDCIIYSSCEPCPMCLGAIYWSRPK
                     KIIYSATRLDAAGAGFDDSYIYDQFALENQDRKIETLCLPVPERMDPFNWWEKWGDKS
                     FY"
     gene            complement(44779..45843)
                     /gene="queA"
                     /locus_tag="PKM27_01035"
     CDS             complement(44779..45843)
                     /gene="queA"
                     /locus_tag="PKM27_01035"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNR05864.1"
                     /translation="MRTKLSQFNFVLPKDAIAQYPSKERDGSKLMVVHRDTGKIEHKN
                     FKDILNIFEDGDTFIFNNTKVFPARMYGKKEKTGAKIEVFLLRELNKEARLWDVLVDP
                     ARKIRVGNKLYFNDKKGNEILVAEVVDNTTSRGRTIRFFHDGSDDDFQAQLKILGHTP
                     LPKYIKRPAESLDEERYQTVFAKEIGAVAAPTAGLHFTKELMKRLEIKGAHFAEVTLH
                     VGLGTFRSIDVEDLSKHKMEAEYFRVPPAAVEAVNKAMANNKKICAVGTTVMRAIETA
                     VSAHGLMKPQEGWTNKFIYPPYDFSIANAMLTNFHLPKSSLLIMAAAFGGTELIMEAY
                     QQALKKKYRFFSYGDAMLIL"
     gene            46075..47322
                     /locus_tag="PKM27_01040"
     CDS             46075..47322
                     /locus_tag="PKM27_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR05865.1"
                     /translation="MFFRLFYEGVVQSFSQLVANKLRTFLSLLGITIGIFCIISVLSA
                     VDSLEDNIKSSFEKLGNDVLYITKMPWNEDPGQNYFKYLRRPNATYQELEAVKTKVRN
                     ASMVALQVFIGGSNLKYQNNSVGGAFVLAVSYDYNDLYKIEYQGGRFLTPFEYENGSD
                     KVIIGAEIAENLFNTLDPVGKSIKLKDRKMEVVGVIKKSGKDLINPADFDRAVIVGYN
                     TAKKLVNVKNNRLWGTNLMVKAAEGSDLEDLKAEVISVLKAERRQKPYEEPNFSINEM
                     SILTNILDNFFGVLNLVGIVIGFFAILVGMFSVANIMFVSVKERTNIIGIKKALGARQ
                     YFILLEFLIEAVILCVVGGILGLSLVFLILKVLSSVFGYVMFLSLKNILIGVSLSIFI
                     GILAGFIPALNAAKMDPVVAIRK"
     gene            complement(47579..47923)
                     /gene="rplT"
                     /locus_tag="PKM27_01045"
     CDS             complement(47579..47923)
                     /gene="rplT"
                     /locus_tag="PKM27_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNR05866.1"
                     /translation="MPRSVNHVASRARRKKLLKATKGFWGARRNILTAAKHTLERAWK
                     YAYRDRKTKKRAFRALWIARINAAARLEGLSYSKLIHGLSSKNILLNRKVLADLAMNH
                     PEAFKAVAAKAK"
     gene            complement(48005..48202)
                     /gene="rpmI"
                     /locus_tag="PKM27_01050"
     CDS             complement(48005..48202)
                     /gene="rpmI"
                     /locus_tag="PKM27_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNR05867.1"
                     /translation="MPKMKTNSSAKKRFRVTGTGKVKRFQANTSHRLRQKSKKAKLAN
                     RDMALVSQADEKRVLRMLALR"
     gene            complement(48280..48861)
                     /gene="infC"
                     /locus_tag="PKM27_01055"
     CDS             complement(48280..48861)
                     /gene="infC"
                     /locus_tag="PKM27_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNR05868.1"
                     /translation="MPYRGAPIKKDYGFRINGEIRVPEVRLVGENLHELVIDNEPPIE
                     PGVYPTRQALRWAELLNLDLVEISPNADPPVCRIIDVNKYLFQKEKKEKELKAKAIKA
                     EVKEIRFGPNTDDHDFEFKVRHARNFLQEGSKVRAYVFFKGRSVVFKDRGELLLLKFI
                     KELEDIAATEALPKLEGKKMFILLAPRKGTVKK"
     gene            complement(49047..51578)
                     /locus_tag="PKM27_01060"
     CDS             complement(49047..51578)
                     /locus_tag="PKM27_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNR05869.1"
                     /translation="MSNKRSPQFQDVIRKSREEANRLRHDFVGTEHFLLGLLKDKDNL
                     VVKVLESLEVDCTELKYKLEKVLQKELANNPPSAAVREDLPLSKHAERIYRLSHLEAK
                     EMKQDTVAPEHLMLSILKHKDSEATRILGELDVDYETFRSELQYVIQEQDLANSDYFA
                     QAQSESESNFEEEGSSKFPRKGGNKSRTPVLDNFGRDITRLAEEDKLDPIVGRENEIE
                     RVSQILSRRKKNNPILIGEPGVGKTAIVEGLALRIIQKKVSRTLYNKRIVMLDLAALV
                     AGTKYRGQFEERMKAIMNELEKSRDVILFIDEIHTIVGAGGATGSLDASNIFKPALAR
                     GELQCIGASTLDEYRQYIEKDGALDRRFQKVMVDPPSADEAIAILNNIKSKYEDYHNV
                     NYSDEAIKACVKLSDRYITDRFLPDKAIDVLDEVGARVHLKNIHVPKHIEEIEQKIEA
                     IKELKNSAVKNQQYEKAAEYRDTESKLQAKLESAKEQWEEEAKVKKYPVDTDDIAEII
                     AMMTGIPVKRVAQSESKRLVNMTDDMKKVIIGQDEAIEKITKAIQRNRVGLKDPKKPI
                     GTFIFLGPTGVGKTELAKALARYLFDNEDSLIRIDMSEYMEKFSVSRLIGAPPGYVGY
                     EEGGQLTEKVRRKPYSVILLDEIEKAHPDIYNILLQVLDDGLLTDGLGRKIDFKNTLI
                     IMTSNIGVRQLKDFGQGVGFATKARVEAAEDNTKSVIQNALKKTFSPEFLNRIDDVII
                     FNSLSEDQIFQIIDLSLNGLAKRLGNLNYELKLTPEAKKFVAEKGFDPQFGARPLNRA
                     IQKYIEDPLAEFILNEGPEEGSILEATLNEEKTGLKIMLHKEATS"
     gene            51830..52210
                     /locus_tag="PKM27_01065"
     CDS             51830..52210
                     /locus_tag="PKM27_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNR05870.1"
                     /translation="MKYAIDKEDRYAILKLNEENLNSLIAPDLKSEFIFLRNEGVRNL
                     ILDLSQVKYVDSSGLSSILTANRLWKDFGSFIVTGVQHAPVQKLIEISRLETILNLVP
                     TVQESIEFAMMEDLQKELEDGSDE"
     gene            52232..53152
                     /locus_tag="PKM27_01070"
     CDS             52232..53152
                     /locus_tag="PKM27_01070"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNR05871.1"
                     /translation="MARFEVLILGSSSALPAYGRHPSAQLVNLHENLFLIDCGEGTQS
                     RLAEFGCKMHRIRAVFISHLHGDHYFGLPGLLTSLNLLGRTGPLDVFCPEGLEPLMDE
                     ILRLGQGELQYKIQWHTLVAGGKKKIFEDRVMEVFSFPLAHRITTFGFMFCEKPGMRK
                     LRPEKIAGRAISYETLKKLKAGIDVVEESGPTYLFSEFTEDLPSPRKYAYCSDTLPLH
                     GLVSIIREADLLYHESTYMEEHLEKAGLNYHSTASQAGAMAASAKVKKLIVGHFSSRY
                     KLLDELLAEARAVFPNSELALEGKKFEVGG"
     gene            complement(53165..53237)
                     /locus_tag="PKM27_01075"
     tRNA            complement(53165..53237)
                     /locus_tag="PKM27_01075"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53202..53204),aa:Lys,seq:ttt)
     gene            complement(53326..53703)
                     /locus_tag="PKM27_01080"
     CDS             complement(53326..53703)
                     /locus_tag="PKM27_01080"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNR05872.1"
                     /translation="MQITAKLVQVLPAQTGQGKNGEWRKQEIIVETEGQYPRKICVSV
                     WGDKLGGLKLIPGTDLSIDFDIESREYNGRWYTDVKAWRVTESDSPGSPASPSAKASG
                     PQAPSSFPPATSAPPEFEDDLPF"
     gene            complement(53710..55044)
                     /gene="xylA"
                     /locus_tag="PKM27_01085"
     CDS             complement(53710..55044)
                     /gene="xylA"
                     /locus_tag="PKM27_01085"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNR05873.1"
                     /translation="MTRKITIGNREYFKGIGKIQFEGRQSKNPLAFKWYEPERVVNGK
                     KMKDHLKFAVAYWHSFCNGNADPFGSVTRPMPWLEGRDPMERAKNKMDAAFEFISKVG
                     APYYCFHDFDLVEEADTLAESEKRLQAITGYAKTKQEESGIRLLWGTANLFSHPRYMN
                     GAATNPDFRVLTYAATQVKNALDATITLGGENYVFWGGREGYLSLLNTDMQREKEHLA
                     RFLQMARDYARKQGFKGHFFIEPKPMEPTKHQYDYDCETVIGFLRAHGLEKDFKINVE
                     VNHATLSGHTFQHELQVAADAGMLGSIDANRGDQQNGWDTDQFTLNLYETVEAMLVIL
                     RAGGLQGGGINFDAKIRRNSTDLEDLFIAHIHGMDLFARSLIIADQMLRESPYSQWLT
                     ARYTSFDQGQGKLFEEGKMTLENLRDLAFQNGEPAQLSGKQELYEQLINLYI"
     gene            complement(55052..57274)
                     /locus_tag="PKM27_01090"
     CDS             complement(55052..57274)
                     /locus_tag="PKM27_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNR05874.1"
                     /translation="MEKSIGKNLIVHPMIRYLTVFLTFLAFLPACKKEAFIPTLVPFT
                     EGENETITKWKNDKLSLFVHFGLYSMYGGVYQGQPVTKGYSEQIMAHAPIPAEEYRAS
                     AAQFNPDSWNADSIANLAYRGGLKSVIITAKHHDGFCMFDSRHSEFSITKASPFKRDL
                     VSEMSEACKKIGLGFGVYYSLIDWNAPEGATPVSGHNADSISEALHRLNVNQVTELCQ
                     HYGPLREIWFDMGTLTPAQSKEIRKVVKDLQPECLISGRLGNGQGDFIVLGDNQIPEV
                     KFDQPWQTPVSINKATWGYRSWQERPDLNKRLQEMLSDLTQVVSKGGNFLINIGPKGD
                     GSVDPFEKELILGMGQWLNLHSEAIYNTRPFPISDKPWGVFTYSPGNVFIHITRIPDE
                     GSLILYGLNNPISGIWSLVNPAEIASYSVTGKVTTIDLGMIPLRFEPSTILRLQYMGD
                     QLDITPAKLVYSDASGKYELNARNANISWGMDGTNYYSAAPSVTRYSWDISNPKDIDY
                     SMMLFYTQEEKNKRIRLKVNGHEEILDLSKYKTFRQAPESTNAVPSIANLKGPYPGLD
                     MNSHPALISYMSPTEGWGSDLTPWNPIPGSEEGKFLQVTMPPMSACYYLFNILASQPE
                     KKICAMGTDDAFMIWLNGKQLYSTGYAHPPDHVRFIELPLDNGSNTLVVKHFNIGGSF
                     NTFYSFKVDQIRFEKEAGANRLEARQNNHIEIELADPDHPGENLGTPNINLVIQEKKN
                     "
     gene            complement(57232..58722)
                     /locus_tag="PKM27_01095"
     CDS             complement(57232..58722)
                     /locus_tag="PKM27_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNR05875.1"
                     /translation="MYILGIDLGSSFIKASLIELSSQRALATASVPDQEMPMRSLHPG
                     WAEQEPEVWWDHIKALVRKILVQTGIPGRDISAIGISYQMHGLVLIDHKGQVLRPSII
                     WCDSRSVPIGDAAFKDLGTSYCLGHLLNSPGNFTASKLRWVIENEPSLAEQVHAFLLP
                     GDFINFKLTGEINTTVPALSEGMFWDFAQDSLALPLFRHYRIPESWCPSLVPTFAIQG
                     RLSSQAASETGLHPGTPVCYRAGDQPNNAFSLNVLRPGEIAATAGTSGVVYGVQQKLA
                     TDEQSRINAFAHVNHTATDPRIGILLCVNGTGIANSWLKKNLGGTMTYDKLNQQAAQV
                     PAGSDHLLFYPFGNGAERMLGNRMVQSHLMNLDFNRHGIGHLARAIQEGIVYALRYGV
                     DIMTANGMTPRAIKAGKANLFLSPVFRQMFSSLLQVPLEFYDTDGATGAARGAGIGLG
                     VYTPSTAFETLHRIEELSPDPALGSVLNELYHTWKNQLEKTLLSTL"
     gene            58830..59756
                     /locus_tag="PKM27_01100"
     CDS             58830..59756
                     /locus_tag="PKM27_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNR05876.1"
                     /translation="MDSQDERLDVRVVFAFAAVYIIWGSTYLGIYFAILTWPPMILGA
                     ARFIIMGVLMYSYIHFVKKERLDLPLFRFGLISGFLMFFIGNGAVILAEKQLPSGLVS
                     IIAGTVPFWLLLLDKRSGAARFRNMLTWLGLILGFAGVILLFTGRLESVRMDSGMIWS
                     YLILIVGTLGWTTGTLYSKYHEVKGSVLAKVSVQVLCAGFLFLLAAWIKGEFRGFSLS
                     QVSDTSWIALWYLIFFGSMIGYFCYMWLLNRVSPHAVGTYAYVNPVVAVILGNVLAGE
                     ILSSREFIALFLITGGLLILYLSKKPWAPRFR"
     gene            complement(59749..61962)
                     /locus_tag="PKM27_01105"
     CDS             complement(59749..61962)
                     /locus_tag="PKM27_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNR05877.1"
                     /translation="MYGLLKNSILFLFFLLVLSACRFGNEETRFANLILIDSTDDHGD
                     ILFKAAHVIPTPRQLEWQKLEMTAFLHFGVNTFTDNEWGDGQEDPKIFLPTNLDANQW
                     VSVLKEAGFRLVILTAKHHDGFCLWPSAFTSHSVKNSLWKNGQGDVVKELADACVRQN
                     MKLGLYLSPWDRHEPTYGSEAYNDYYKRQLRELLTNYGPVDEVWFDGAVGEGPNGKKQ
                     VYDWQGYFRLVRELQPQAVIAIMGPDVRWVGTESGYGRETEWSVIPIQDSIPVTADDL
                     HDNMVKPVLDETREEIANLEQLGVAGAMKWYPSEVDVSIRPGWFYHASEDRLVKSPEK
                     LLDIYFNSVGKNSVLLLNIPPDRQGLIPEKDATILKVFSNSLRQIFSNDLARAARADD
                     NDFTISHLPKNTNDGHRDTYWSPDEGNAAPFISFEWQKPVWFNILKICEQIELGQRVA
                     GFQLEILEDGQWIKKASGTTVGYTRLLRFPWVRTQKARIIFTDFRNTVNISEIGFYQN
                     MPEVTLTPPGKPFEDSLVVTMSTNDPEANIYYSQNYSLPSVASNLYTGPVKLNHSSPI
                     LAIAINPDGLSGFIRDEVYSKAKYKARLLTTPSPQYMSEGALVLCDGLKGDLDFKSKK
                     WLGYENQDLETIFDLGQSRPVNSILIHSLSLVKKGIFPPLQVSIYGSGDGENFQLIKT
                     KVENFEAHPEDGLVTWAVPRIDRRLRFVKIVVSNRLPADDDPVNGFLFVSEIEII"
     gene            complement(62015..63067)
                     /locus_tag="PKM27_01110"
     CDS             complement(62015..63067)
                     /locus_tag="PKM27_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNR05878.1"
                     /translation="MTYVKPAFRHPEAAKNKLGYTRKEYEGALSTLCAGCGHDSISAA
                     IVQGIFELSIPPHRVAKLSGIGCSSKTPTYFLGNSHGFNSVHGRMPSVATGAYLANKD
                     LIYLGVSGDGDSASIGMGQFVHAIRRNLNMVYIVMNNGCYGLTKGQDSATADPGSINK
                     SGHTNLFETIDMAGMALELGASFVARSFSGDKEQLIPLIKAAVSFPGFAFIDVISPCV
                     TFNNNKGSTKSYDYVRDHLETVAELDFVPLAEEITSDIASGESGRITMHDGTYLQINK
                     LALDWDPTDRISSLTKIHRSRMNQEVVTGLLYYEAERKNLHDILNTVDTPLNALEYYE
                     LCPGNSVLQTINAELR"
     gene            complement(63033..64895)
                     /locus_tag="PKM27_01115"
     CDS             complement(63033..64895)
                     /locus_tag="PKM27_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNR05879.1"
                     /translation="MSRIEKINDCVIRFANVNGTGSASANNLFAKAIFRMGVPVSPKN
                     IFPSNIQGLPTWYEIRVNEKGHLGRRNGIDIMVSINPQSLAKDIASVSAGGYFIYDNT
                     KPMPKGLLRDEVNNIGIPMTEICAQNYADPRQRLLFKNMVYVGAVATLLDMEMAVLEQ
                     MVSDQFKGKEKLITPNVKALHLGRQYLIDHFELPSKFRIERRDLNKDLIMIDGNSACA
                     LGALYGGATVAAWYPITPSTSLMSAFDEYASSYRLDPKTGKKKFAFVQAEDELAAIGI
                     AIGANWNGARGFTATSGPGISLMSEFLGLAYFAEIPLVLIDVQRGGPSTGMPTRTQQS
                     DILACAYASHGDTKHPLLFPSTPRECFEMTAEAFDLAEQLQTPIILMSDLDLGMNEHS
                     SQPLVWDDRRNYKRGKVLNAAQLDEIKEFGRYLDVDGDGIPYRTIPGTHPTKGSFFTR
                     GTSRDEMARYTEESEPYVRNMDRLLKKWDTIKNYLPAPEWQNQEQENKMGMLYFGTSE
                     SSSLEAADLLEEKGIHLDLGRIRAFPFHPSIADFIQDHQKIFVVEQNRDAQMRTLLIN
                     ELDVDPARLIPVLNYDGLPITGMLIHDKIMEHIGYASTTLTDQHDLRKTSFSAS"
     gene            complement(64883..66673)
                     /locus_tag="PKM27_01120"
     CDS             complement(64883..66673)
                     /locus_tag="PKM27_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR05880.1"
                     /translation="MEPTNIKDPEYFHKVVDCQYACPAHTPVPEYIRLIAAERYSDAY
                     MVNWESNVFPGILGRTCDRPCEPACRRGRVEKEPVAICRLKRVAADFKGEVVSRMPSA
                     PETGNGKRIALVGAGPASLTVARDLAPLGYEIDLYDEQPLGGGMMRSQIPAFRLPEEV
                     LNEEVGYVLGIGGISTHFKTYVHSLKDLLESGYDAVFVGTGAPRGKDLSGLPGRKEIQ
                     ANIHIGIEWLANVAFNHVDKIGKKVLVLGGGNTAMDCCRTSRRLGGTDVKVVVRSPFD
                     EMKASPWEIEDAQHEDIPFFNLHNPKAFVHENGKLRGMLFEKVAAQYDDQGKRSLVPT
                     GEPDIFFEADDVIIAIGQENAFPWIEKDLGLEFDKWGMPLVDKITFGSTLPRVFFGGD
                     AAFGPKNVITAVAQGHQAAVSIDLFCNGEDLYNRPDPLTNLVTQKMGIHEWSYDSTVV
                     TDLRYKVPHASKSETLTNRKKEVELGFDDLLAFKEAQRCLNCDVQTVFTAERCIECDA
                     CADICPTSCITFTHNGEEEDLRGRLNAPAQLLSQDLYVSSPLATKRVMVKDENVCLHC
                     GLCAERCPTAAWDMQRFLYNTAKAGTPCHV"
     gene            complement(66878..67513)
                     /locus_tag="PKM27_01125"
     CDS             complement(66878..67513)
                     /locus_tag="PKM27_01125"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNR05881.1"
                     /translation="MALRLGDLVPNFKAQTSQGEIDFFDWLGNSWAVLFSHPADFTPV
                     CTTELGYMSKLKPEFDNRNVKILALSVDPLASHTTWIHDIEETQVTKVNYPLIADENR
                     HVAELYDMIHPGAAEKTTVRSVFIIGPDKKLKLSITYPPSTGRNFDEILRVIDSLQLT
                     ANSGVATPVNWKNGEDVIILPSISDADAEKRFPKGFRKIKPYLRYTPQPNL"
     gene            complement(67597..68775)
                     /gene="moeB"
                     /locus_tag="PKM27_01130"
     CDS             complement(67597..68775)
                     /gene="moeB"
                     /locus_tag="PKM27_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNR05882.1"
                     /translation="MTVNGEIKFSPEELARYSRHLILPEFNIEGQKKLKQAKVLVVGT
                     GGLGAPLLLYLAAAGVGTIGLVDFDVVDDSNLQRQVLFTRDDLGIPKVIAAKKRLEAL
                     NPFIKIIPHQTALTSDNALDIIRDYDVVADGTDNFPTRYLVNDACVLLGKVNVYASIY
                     RFEGQATVFNYKNKAGELGPNYRDLFPTPPPPGLVPSCAEGGVLGVLPGILGSIQASE
                     VIKVLSGVGEPLSGRLFLFDAATFETRTLKIKRRKDNPLNGENPSQTGLIDYQQFCGF
                     EKPTDQAEKLPVKSINVKQLQELIDRNANIQIVDVREAYEYEIANIHGELMPKSEVTS
                     HVQKISKDKQVIVHCRSGKRSADVIEMLQEKYGFDNLYNLEGGILAWAKEIDQSMPTY
                     "
     gene            complement(68804..69079)
                     /locus_tag="PKM27_01135"
     CDS             complement(68804..69079)
                     /locus_tag="PKM27_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNR05883.1"
                     /translation="MAKIIIPTPLRKFTSNQAAVAVQEGTVLESIRDLTQQFPDLDKH
                     LFNEDQKLRGFVRIFIGENDIEDLEGQETRVNPGDTISIIPAIAGGL"
     gene            complement(69086..69523)
                     /locus_tag="PKM27_01140"
     CDS             complement(69086..69523)
                     /locus_tag="PKM27_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNR05884.1"
                     /translation="MIMDIEIKPSVLKAIHDIAEKTYPYECCGFIFGEAGQPLRAEEV
                     REVVNNSNENHKVHYTIPPLEFMRAERYALENNKLLVGVFHSHPDHPPRPSARDLEDA
                     LPELSYFITSVHQGQALDTRSWRLSEQRVFQEETLKINYHSNN"
     gene            complement(69520..70446)
                     /locus_tag="PKM27_01145"
     CDS             complement(69520..70446)
                     /locus_tag="PKM27_01145"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNR05885.1"
                     /translation="MNETITWSNTDLVKSVESLGRWVGGTPLFPLHHFSTEKVKIFAK
                     LEWQQFGGSVKSRPAYNMLRQAIHTGELQPHHTILDATSGNTGIALATLAASLGLKIK
                     LVMPENASRMRQQILKKLGAELVLSSPYGGTDESRQLALAIYAKMDQPYFYCDQYNNE
                     NNWKAHYHSTAPEIWRQSSGYITHFVAGLGTTGSFTGTSKRLKTYNPAIRCIALQPDG
                     PLHGLEGWKHLETAEVPGIYDPGTPDQILQVSSYDAFKLIDEVAKKEGLLISPSAAAN
                     LTGALQVAKTMEEGTIVTLFADDASKYQEVYK"
     gene            complement(71030..71389)
                     /locus_tag="PKM27_01150"
     CDS             complement(71030..71389)
                     /locus_tag="PKM27_01150"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNR05886.1"
                     /translation="MENDGTQRNLSVYSHKVKAGRKRTYFFDVMKTRGNDYYLTLTES
                     TRRSDGNGYIRHQIFLYKEDFNRFVEGLQDVVNHVKTNLLPDFDYDQFAHRDENHDNH
                     DDSIHDEDKSKSDQMEW"
     gene            complement(71439..73250)
                     /locus_tag="PKM27_01155"
     CDS             complement(71439..73250)
                     /locus_tag="PKM27_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR05887.1"
                     /translation="MSAILIVASLKQLWTLNTYFLKYKWRFLAGIVFVLLMNYFRIVQ
                     PQMIRDALDLVVEELKNPDTESRQRLAPQLVRFGLIVFGCAVLMGIFMYFMRQTIIVI
                     SRLIEYDLRKNIFTHYSTLDQSFYRRQTTGDLMSRITEDVSKVRMYLGPAVLYAIDLA
                     ALIVLVVFSMVKVDLTLSLLTLLPLPFLSISIYYVSTLINRKSTLIQRQLAKLTSIAQ
                     EVFSGIRIVKSYTQEKAMSGFFREETEEYKTHTLDLSRVNALFFPLIMLLTGLSTVII
                     VWAGVYRVQSGAVTAGNIAEFVIYINMLTWPVTAIGWLASIVQQAEASMKRINEFLTL
                     KPAVESTGTYKPEKLQGRIEFKEVSFIYPNTGTLALDRVSFVIEPGSRLAVIGRTAAG
                     KTTIAELLLRLFDVTSGEILVDGVNIKDYDLNALRKKIGYVPQDHFLFSETIHNNIRL
                     GAPSLGEEVVWQTARHAAIEEEIRQLPDKMQTILGERGVSLSGGQKQRVAIARALAMN
                     PDILLLDDCLSAVDTRTEKNIIQAFEQVLNHKTAILITHRLNHLMGFNQILVLHKGRL
                     EESGTHDELLRKKGLYYKLLERQKWEESNAPGEVEVF"
     gene            complement(73323..74453)
                     /locus_tag="PKM27_01160"
     CDS             complement(73323..74453)
                     /locus_tag="PKM27_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNR05888.1"
                     /translation="MKFKPFPAFFRYTLHQRKSQFALLFMVFILGLVYLYIHFFPPQS
                     GYVILPLEKGEDVNPGPSSLESPVESKTSVPTALRIDPNRVTLDLLLEAGVPKRVAGT
                     WMKYLAKGGKIKSSQELGKIYGMDQSLLDKLTPHLVFENAGKAKGTALNLNLSLNLNL
                     SLNPNTASVRDLTAAGLPLKVAYTLDNYRKKGGRFLKPADLSRVYGLQDSLLQRILPF
                     LVFEQEEPDANPHSENPIADEIPPSPETVDLNRSDTSAWERLPGIGPVLARRIINFRE
                     ALGGFHERGQLKEVYGLPDSTYHAIEPYLIPSPITRLIRVHLDLPLKKAHPYLSVKES
                     RILANYIRQHGPFKDPAAVIRIQAFDSTFWKKLLPYLDFSTP"
     gene            complement(74450..76453)
                     /locus_tag="PKM27_01165"
     CDS             complement(74450..76453)
                     /locus_tag="PKM27_01165"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNR05889.1"
                     /translation="MRILIPFALGILCSNRTTGSLLLGLAVLVPSLLAMIGAHRRAKR
                     QVMPAPWFGLASLGAWWALGFAWAALRDDRTLPNHFFDPEKQYQELVVEIHQVDEKER
                     YWNGYSRTFLEIGDSINRNVHGNLLIRIPKPGSYNPSSGDVIQARFRIQEPFKPSFPF
                     EFDWNLFLHRRNIHHFTYLDTADFRVVHHPGPGLLESLRARLDGYIREAVPSDRDYPV
                     ASAMLLGSYKKMDQDLFNAYSATGAVHILAVSGLHVGIMAHIFQYLFGFIRIGKKKPV
                     QLQALLLIGIIWAYALLTGAAPAILRAALMFSFLTVSRLLLRDSSPVNILAAAAFVLL
                     CINPFDIYNIGFQLSFLAMAGLFLLYEPFRTLITVENRIVQVLVKVIAASCAAQLAIY
                     PVVGFHFHQFAFYFWLTGLISTPFSYLILGGGLFTLALWPLAGHLVPWISIPFRYGVL
                     WMNETVSWIYTLPLGKIQGWWPSLADTLFLILLSGGMAYVHFQKTRFSFNCMLLCGIL
                     FLSVLILEEKRNQTKTELVAGTHKNRPWLWIKHRENAFLYLIQDSMKIPRSYLEKYKL
                     REENIIYFHPGKQSLLSGPLRLEGNQIIFGEKHFEIALEDQKTKSEYSKFQPLLKNRP
                     AATPSITTQKKPGLPDLISFAADLKDGPSPRPVESPYLRIRIK"
     gene            complement(76514..77767)
                     /gene="fucP"
                     /locus_tag="PKM27_01170"
     CDS             complement(76514..77767)
                     /gene="fucP"
                     /locus_tag="PKM27_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HNR05890.1"
                     /translation="MQSLKKNYLLPFILVTSLFFLWAFLHNLNPILIPHLKKACQLTD
                     TQSALIDSAVYLGYFLMALPAGWFMHKYGYKNGILLGLGLYTVGALLFYPAAGARSYT
                     MFLTALFIIASGATFLETVANPYITKLGDPATSEQRLNFAQSFNGVGAFLAPIIGGQF
                     ILSGIEKTGEELAALPVDQLNQYLSFEASSVKIPYLVMAAVVFTVGLFFLFSHIPDIK
                     EEDSSDTGHSFSLKVFRHRHVLWAVIAQLFYVGAQVCVGSFFIRLTRFLIDMPEKTAA
                     YIWGSVAMVGFMVGRFVGTWLMKYVAPARLLSLYSLINIGLLALALVTSGKVAVYAIA
                     AVPFFMSIMFPTIFALGIKGLGEETKIASSFLVMAIFGGAVFPLFMGMISDSTGSIQK
                     AYIVPLLCFVVVWFFGRFKYSPSSS"
BASE COUNT        19073 a        19127 c        20081 g        19656 t
ORIGIN      
        1 cggaggcaga atctttggac atgagtttta ttgatgaata cgcagaagat acgggctctt
       61 cctgcagggt gatccaccgg atttggacca ttacggaccg atgcacctat cagccttcgg
      121 caccgcatgg gcctgatgtc attctccatg acagcatggt cgctgatcct gtcgaccggt
      181 attgtgttta ccgggcgctt aaagacaacg gggatggcta tatgcgatat acccagcaaa
      241 tccgttttgt ggaccggaca ccgccccggt tatcgttcag ggataccgtg atctatactg
      301 gcgctgcttg cgtttcagag ggtttttccc tgttcccaac ggtatcagat gactgcaccg
      361 ggactgcttc cataaggatt caggcctggc ttgacctggg tatggacaat acccttgacc
      421 aagagtttcc cccgggtatt ccattggata taccgggagg ctttggacct ggaattttcg
      481 gactccatat tattgcggag gatctatgca gcaaccggga cacctgtata ataaagattg
      541 aaataaagga agggggagtg ccatccgcat tttgtttgcc cggcacagca attattgaca
      601 ttcctgcctc aggccaggtg accatttacg cccgggactt ggatgccggt tcgttggcag
      661 gatgcacgcc gggccccctc aggtttagtt tcagtccgga tccggcggac agcatcaggt
      721 cctatacctg tgacagtgct ggtaccaaaa cacttccaat ctggctgacc aatgaaggcg
      781 gctttcaggc acagtgcgag acccaaatcc gactgcttcc cggacccgat gcttgtatcc
      841 cttcagctga aatccgggta aagggcaggg ttttcacagg acttaagatt ggaattaaac
      901 aggttaacat aggttttgaa cccctggaaa ctgacacgct gaccgatgcg gagggatatt
      961 acagttcgag ccctttgcca gcggggctgt ccctgaccgt gagaccatcc aaaacggatg
     1021 gtcccttgaa cggggttagc accatggatc tcctgttgat ggaaaaacac atcctgggtc
     1081 aaagcctgat cacggatcct ttaagcctga tcgctgccga cgtcaaccgg tcgggatcag
     1141 tgacggtctc cgacctgatc gaactgaggc gcctgatcct gggctcgata aacacctttg
     1201 tgaacaatac cagctggcgg tttatgcctg ccggccacct gtttaccaat ccggccaaac
     1261 cctggctttt cccggaaacg atcgaattta agaacctcat gggtccaata accaatgccg
     1321 actttatcgg ggttaaaacc ggggatgtca attatacggt tggagcgggt ttgagtggtc
     1381 tggaatccag ggatcgtgat agggttgact tgaaagtgga aaccacagaa gaaggaggtg
     1441 aactgaagat ccgcattttt ttggctgaac cattgaccca ggtggacgcc ctgcaattgg
     1501 gaatcgcctt gccgggaagc ctgcaatcca ttgaatgctc cgggggccag ctttcgatcc
     1561 gtccggacca atataacctg ctccatggaa atcaaatccg aatagcatgg gtgaacggac
     1621 agggcaagaa tttaaatgaa acagaacccc tcctggaatt tacaggtaaa aagggaggtg
     1681 cgttattgca gacgcaactt atcgactctg gaattttcga atccttccta agcatttcag
     1741 gtaaaccaag gactttgtac tgccaagcgg tgaaggctgc cgggatccag ttgagagcat
     1801 tcccaaatcc atccagtgac cgggtgaata tagaatttta tttgccggag ggagggcaaa
     1861 atgaaatcga aatttttacc atgaatggaa aaaaaatcta tatgtcggaa agcacattat
     1921 acccgggatt aaaccgtata ctattgagaa aaagtgattt aggagggcca ggaatctatt
     1981 attttaaaat tcgtacacga catacttttg agaacttaac atttatattg ttttaaacaa
     2041 gatagttatg aataataaaa gatgctaatg ttaaaaatac ccaaaaaagg gtataagtat
     2101 ccctaagcca taaaattata tttgttggac ataagacctg aattttacac atttttcttt
     2161 ctgcaaaacc catagtctca tgaaaaaaac gatactttta tttactattg ttttttgctg
     2221 tttctcagtt caaacaagca tatcccagat attacaccct gattatcagg acacggttac
     2281 cattattatc aacggagata cgacctacct agacaatggt ggcgcaggaa attacccgga
     2341 ctgcactgga gcaggcggga tgccttctga cgcgattgat acttgcacga gcattctaac
     2401 gatttgtgcg cccggaggaa ctctctgtgt gatttttgac acccttgatc tcttcgctgg
     2461 gggtgatgca ttgtctgcat ttgatggtat cttgggcagt gcaagtatag gatcttttca
     2521 agaaggagat actattgcca gttctggaga atgtctgaca ttttcattct atggaacctc
     2581 tttaaacaat gacaccggtt gggtagcgcg aattatctac aaactccccc ctcccgatcc
     2641 cggtgaatgc aacctggtct gcaatgatca tctgaacgtt tccatgcccg cagatacctg
     2701 ttataaagtt atagaacccg acgatctgtt agaaaacccc ttggagggtt gcatttacca
     2761 ggtggccctg gctcatcctt tcgggactgc gcggcctccc tttattgcac ccgatgcggt
     2821 caaccgctcc catattggat acacctttat atatagtgta tatgtactcg atgaaaacag
     2881 caaccgagtt aactcctgtt ggggttatgt gactattgag gataaagctc cgccgcaacc
     2941 catgtgcaag gatgcaacca tttcctgttt ccaggtcgaa tacctgaaca ggctggtcaa
     3001 taccgtggtg gacaactgcg cacagtccgg caaatcggtg atcaccagca tgaccttctc
     3061 ggattgggga tgcgacagcg ccgcctatat cgggcaggtg gtccgcacca tcgtttcctg
     3121 ggatacctgg ggcaacagcg ggacctgcaa cgacaccctg accatccgta aagattccct
     3181 ggaactggtc aaatgtcccg atctgatcga gctggaatgc gaagattgta atcccgccat
     3241 tgtattcagc gatgataaag atgatccaaa ttatcctgac ccggatttct tgctcgaact
     3301 tcaggaagac ggatgcctca gcgacacttt gatgatcgtg cctatgatcc gggattctgt
     3361 tcttgtagtc cagggcgata cctgctatta tgtggattcc tgtgtcaata tgtggcccgt
     3421 cctcggcggt ttctgtaaaa tgaccgtcgg atatacggat caggtcctta ccatctgccc
     3481 ggatggctcc ggtttcaaga tccgccgcga atggaggatt gtggattggt gccgcaacgg
     3541 ggaagacact gtgtgtgtac aatacatcaa ggtggtggat gatgaagggc caagattcac
     3601 aggggatcct gaagatctct tccattatgc ttttgcttcc cagcatgact gccaagccaa
     3661 tgtcaccatc ccggggttgg agtatgaaga ctgttcggat ggagtgatcc agacttatgt
     3721 tatcgaatac cttgacgaaa gccacccggg taaagtcagt gtgctcacgg gaaccctgcc
     3781 tgcaacgatc aaactgccgg cttcagcggc tacccttctc ccacccggtg aaggcgaagg
     3841 cgatgaaatc tttgcaatag gcggactgaa atgtttttac ggagacgtga ccctgcagga
     3901 cccctgctac aatgccagtc atgttcctat attcatctgt gttattgaca atactcctcc
     3961 cacaccggtt tgcgatgaat acacccagac tacagtagac ccggccacct gttgggcccg
     4021 ggtttatgcc catgacctgg acaacggcag ccgcgacaat tgttgcaata ccctgcattt
     4081 tgcagtggct accatggcag atatcgatac ggccacggct catcttaaag ccgatctcga
     4141 agcctgcgga tcttcttatg tatggcataa ggataacaaa gcggcccacg aatggctgat
     4201 caaccaatac atcaactgtt atgtattcaa ggattatgtc gacctgaccc agtgcggcaa
     4261 caaccaggtg gtcctgcggg tttatgaagc gtgcgggata ccgagatacg atccccacgt
     4321 attcccctgc agcccccacg agtggttctg ttacaacgct tacaccacgg accggatgtg
     4381 gagaaattac ctgaaactgc atgtcgacgg tcaaaaactg gatccctgtt ccgcacccgg
     4441 gcaactatac tgtaagaagg attatttcaa atggctgatc aatgtcgcag acagcgatgg
     4501 cgacggagtc agtagtttta ttcagttgat tatcccggct tacgatggag ccactatact
     4561 tccaatggac attctcgccc ttttgagcat acccagtatc tgcacgccat tcttattcga
     4621 tgatcaaatc gcctttggcc tccgcgccgc cttaccccaa aacgcatcgg acttcagcgg
     4681 ggctccaggc aacacttgca gccaattgcg ttacaacgat tgtatggtca acatcctggt
     4741 ggacgacaaa caacccgccg tctgtgaaga tccggatgat atcttctggt tctgcgacaa
     4801 tgtttcggat caggtgacgg tcaatggtca caggcggtat tacaattact ggaggtctga
     4861 ccgggtggaa tatgcggata aagtgtgcta cagcaatggt ggagacactc cctggcttga
     4921 tgggaccgca gagcacaaag gatccaaaga ctacgatcct acggagaatg ccgaagacgg
     4981 ctcctgttat tactatcctg aaaaatcagt ctacaacgaa atcgaatgtg tggaagaatt
     5041 tgatgatgac ctgaccgaca ccgctgaccc gaccgggaaa gcatacggct ggtacggctg
     5101 taatatctac ggcgggtatc acgacggcaa agacaattgc atcagcagct actccagaca
     5161 acttggtctt gaaggtacag ctgctggccg gaacggaggg tacaacagct gggcaccggt
     5221 ttattgccac acctggttat gccttgaccg gaaggattcc ggcggtaaga tcgatcccaa
     5281 atcggctttc tttgagcctg tatttgacag caatgccgac ggcgaactcc tggaaccggg
     5341 agaaggcgaa ttcctggtat gggacaactg ttccactcct gaactcgtaa gctccgtgga
     5401 tgatggtttc atcgacaatt gcggcaatgg ctggatccgc cggacctgga ctttcagcac
     5461 cggctgcgaa aacgaaacca tcgtttgcga ccagaaaatc attaccaaac accggtccga
     5521 ttttgaagtg gtattccctg ctgacttaat cgttacctgt gaagaggatg accttggttc
     5581 agatggcaaa ggcggaccca gtccggatgc aacgggccgc ccgatgatca tggatgacga
     5641 ttgcgaactg gtcggagtga attattctga cgagaggttt gatgtgatcc cggacgcctg
     5701 ctataaaatt gtcaggacct ggaaactgat agactggtgt aaattcaatc ccaaccagca
     5761 cgaacgcgat cctgaagtga tcgttgacga caggctggtg gccgatccgc tcaacaggta
     5821 ttgtgtttac cgcaatttga aagacgatgg ggacggcttt atgacttata cccagatcat
     5881 caaagtgatc gacgagaccg ctcctgaggc cggagtgatt gccgatacga ccttctgtgt
     5941 ttacacgggt tatgacggtg ccggtgcgga agatttctcc gattgcactc ctggtaccta
     6001 taccagcccc aatttcagcg ctaacgatca gtgcactcct tccgcggaga tccgtttccg
     6061 ttggttgctg gatctggaca gtgatggatc gatcgatgat ttcagcggtg tcggtgtgaa
     6121 gtattttacg cacagtgaac tgacgcctgg tacccatacc gtttccgtca ttgccgaaga
     6181 ccgctgcgga aatgccgata ccagctattt cgaaattacc gtgaaggatt gcaaacggcc
     6241 gactccttat tgttacaatg ggattgcgac ggtcatcatg ccttcttcgg gcagcgtgac
     6301 catctgggct tctgacctgg atgcaggcag ttttgacaac tgcaccgcgc acgaccatct
     6361 tattttctca ttcgatgaag cgggcctgca gccgagcctc accctcacct gcgattccat
     6421 cccgaacgga gtgagtttca ccaaggtgat ccgcatttgg gtgaccgacg aggaaggaaa
     6481 taaagattat tgcgaaacgt ttatcgtatt gcaggataat gcaggtaacc gttgtccgga
     6541 tgtggccgga gtaaacggga ccatagccgg taaagtggct acggaaagca ccgagccggt
     6601 ggaacatgtg attctggaag ccaagtccgc aggacagaac ctacctgtct ttaaaacaga
     6661 cgtgaagggg acttattcct tcagcggcct gcccatgaac aaaaactaca gcatcatccc
     6721 aggcaggaac gatgatccga tgaacggggt gagcaccatt gacctggtat tgatccagaa
     6781 acacatcctg ggtgtgctcc cgctgaacag cgcttacaag gtgattgctt cggatattga
     6841 taaaagcaac gatatcaccg ctgttgatct ggttgaactc cggaaactga ttcttaccgt
     6901 atacgacaaa ctcccgaaca acacgtcctg gaggtttgta ccgaagtcgc atactttcaa
     6961 caatatcaat aatccttggg gcttccccga gaaaatagat atctcgaacc tgacgaaaga
     7021 tgaactcaac cgggacttcg ttggaatcaa ggtgggtgac gtgaatggca atgttgttcc
     7081 ccacagttta cttggagggg aggcaaggga caatgggtct agcctgaaat tccggacgga
     7141 agaccggctg gttaaagcag gggaagaaac cgtgatcgcc ttcacggctg ataatttccg
     7201 gaatatcgag ggataccagt tcagtctttc actcaaaggc ctcgaattga aaggggtaaa
     7261 gaatggcttg ttaaacctga atgaaaacaa cttcggtttg acccgcctcg gggaaggata
     7321 tatcaccact tcctggaacg aaagcaacgg catcagtgcg ggaagcaatg agatcctgtt
     7381 cagcatcacc gtcaaagcga cgaaagcagt gcaattgagt gaaagcctgg tcatcaattc
     7441 aagatttacc agggccgaag cttatgatgg agtgaagagc atgggggtca gcctcgaagt
     7501 gggtcataaa gcaggttccg gatacgccct gcatcaaaat acgcccaacc cgttcaaatc
     7561 ggctacggtg atcgcttatg aattgcctaa agctgaaaag gtaaccttga aactcaccga
     7621 tgtcacgggg aaaataatcc gtgtttatca gcgtgaaggc gtgaaggggt acaatcagtt
     7681 gaaagtcaac cgtaatgaag taggtggggc aggggtgctc tactacacga ttgagacgaa
     7741 agattttggc gctacaagga agatgatcct ggtcgattga tcaggtcttc cttgacgttt
     7801 ttggtcggaa tatttacaaa gagccggagg gttttacctc cggctctttt ttattttgcc
     7861 aaattttaaa aattggaacg attttggcag aataagttcc gtgagataga cccctgtgcg
     7921 gagaattaac tcaaaaccaa agcgttatta acaattaaag catttttaaa atcgttattg
     7981 aaggaagccg gcaaagccca tatctttgct gacttttgat aaattataat ttgtaatgtg
     8041 ttaaacaact gatatccatg aatttcctac gcagtttgat cttcttcctc ctttgctcct
     8101 cttcccttat ggcgggtaat cccaaattca ccatatcctc cgaagcaggg aatacaaacg
     8161 atgaagtcac cattaagttt acggtgagcg attttataga tatcgcgggc atccaatgga
     8221 ccctgcgctg ggatcccacc tgccttcaat acgtcagcat tcaggatttt aacttacccg
     8281 aactcaacat caactccttt aatgtttcaa agacctctca aggccagatc aacacggtct
     8341 ggtattacca acagggagct tcaaaaaata atggggatgt catttttacc atcaaattca
     8401 agctgatcgg tgcagatggg agatcctgcg gtgtggagtt ttcaaacaat cctctcccta
     8461 ttgaaatttc ggataaaaac gccatgaagt gtacggaaac cactacaaac ggcaaagtgg
     8521 atatcggtaa tgtgactacc ccaaccggtt ttgtgatcag tgcaggcaac acccaggctg
     8581 cgaaggacca ggaagtctgt gtaccggtgc gggtctccgg atttaaaagc gtaaacgcca
     8641 tgcagttttc aatgggctgg gataccagca agatcaaatt caccaaaatc cagaattttt
     8701 ccggtctgac ttcctttgac cagagcagct tcaacctggc actggtcaat gccggtaagc
     8761 tggtatgtgt ctggtttgat cagaatacga ccggtcagac ggtgaccgac aataccgcga
     8821 tttttgaaat ctgttttaga tacacgggag tttgccccgg atcaggtacc gttaatttta
     8881 cggatgatcc tactaaaacg gtcgttaatt ccacgacagg cgctttaacc gtcaccaaaa
     8941 catccggaac catcactgct tccaactgca gttctgcact cgccgtgagt gttacgaaaa
     9001 ccacccatcc ctgtccgggc cagaacaatg gcggtattga gatcagtgcc accggaggtt
     9061 cgggtgcaat cacctacacg tggaccaata actccaccag ccagaatcta agtggtgtag
     9121 gcgcgggcag ttataacgtc acggtcaaag acggaaccgg agccaccgcc acgctgaacg
     9181 cagcggttaa cctggccgca ctggcggtga cctcctccaa aacagatccg gcacagggac
     9241 aatccaacgg cgtgatcacg ctgacggtca ccggtggaaa caccaattat acctataaat
     9301 ggagcaacaa tgcaaccacc aaggacatca acaatctggc agccggttct tattcctata
     9361 ccgttacaga cgcaaacaat tgtacggtaa cgggaaccgt ggctcttgga ccggggagta
     9421 ccgtgctcga catcgcttct gtgacgccga ccaatatctc ctgcaagggg gacgctaccg
     9481 gggccattga tattgccatc agcgggggga ccagcccctt cacttatgcc tggaccggtc
     9541 ctggaaattt ttctgccaac accgaggacc tgaccggcct caaagcggga acttacaaag
     9601 tgaccgtcaa agacggtgca aatgccacca agacttcaaa tgatatcatc atcacggagc
     9661 cggccaccgc gctgaaaatt acggcaaggg taaaacccgt gtccatcgcc ggcaacgatg
     9721 gagaagtcat tacgacggtg accggcggga cccctgcgta tcaataccgg tggaacaacg
     9781 gttcgaccaa cagcagcctg accagcctag cccagggaag ctatagcatc acggtgaccg
     9841 attcgagggg ttgcacggcc aatgccacct ttaatgtcaa tccttcggcc ggaagtgaat
     9901 gtttcacctc ccagcgggta tttaccccca atggggatgg aatcaatgaa ttgtttgtca
     9961 tcaactgtgc ggaaacctta tccaaccagt tgctggtctt caaccggtgg aaccaactgg
    10021 ttttcaaggc agacaattat gcaaacaact ggctgggtac ggatcagcaa ggcatcaacc
    10081 ttccggatgg tgtttatttc tgggtattga aggatgccag cggggtgacc agtgcagtgt
    10141 ataaaggcca tgtcaccttg ttgaggacgc tgaattaata atgtcaaaat ctacccatcc
    10201 cggtacaaaa acagtaatat gaaaaaaatt ttattcctcg cagtatgctt ttggtcggcc
    10261 aatctcggtt tgaaggcgca ggaagaggct atttattccc agtatttatt tcatcctatc
    10321 ctcgtcaatc ctgcttatgc cggatttctg cctgaccatg agttgttgtt caatttcaga
    10381 aacacgcatg ccggtttccc gggttctccc caaacctata ccctggcatt taatggcccg
    10441 atagcgccta aactcggttt tgtaggactt ttaaacaacg ataaggctgg cgatctgaac
    10501 cggttccggg gccaggccgg cctggcctat atgtttaaaa tgggtacggt caagatgaat
    10561 gcagggctgg cggcacagta tcagaaattt caactggcca acggcgccat cctggattca
    10621 ggcatcgatc cctccgaccc tctgatccgc gccgccgccg acgggcttaa ttttttcaat
    10681 acctccatcg gggtttacgg agaaaacgac gggaaactct tttttggctt atcggtcgta
    10741 gacctggtca ggacgaggat tgatgacatt caatccccca cagatgaaag ttccttcttt
    10801 aaatattata atgcctgggt gggctaccgc tggaatatcg ccaattataa cttcaccgtt
    10861 gaaccttccg tgatgatcaa gcggctgaga aatgtgccct ttcaaaccga ttttaatgtt
    10921 cggttgtcat tcctggatga tcaactgttc ggtggattga cttatactgc aggcggtttc
    10981 agtaaaacgt ctttcctgct cggaaccagg atcaatacct tccgcttgta ctattcttat
    11041 gatgtggccc tggaggattt ccagaaatac aacaacggtt ctcatgaact ttccctttca
    11101 ttcaggatac cttcggtagt gggaaagaac aaccaataag attttttgat gcttcataat
    11161 gagcccgggc atttccgggc tttttttttg taatgccctt atgcatacca tttagtattc
    11221 atatttaaga tcatcatact catccatcaa tacatggggg acgcttgtat atagaaatct
    11281 ggtggaaggg gaataaattt aacttaaatc aatccctgaa caagacgcca tttcctacat
    11341 ttatgccacc ctccttcttt ttatttatta attcaaggat atactatgaa aaagttgatg
    11401 gtgtttttta tcggggtggc catgttctgt atcccgagtt tagctaaagc ttcactggaa
    11461 atttattgtc ccaatgataa agaaatcacc tatgccgctt atcaggccgg caaatactat
    11521 gacaaaccgg tggtccacgg cggatattat actttatatg gcccctggat ttataaacac
    11581 ctgaattgcg gaagcggtta tgtagaagtg cgctgggaga tcctggacca ttacggcaga
    11641 aaatattatt gcaatcagac catctgggtg accaacccgt cttcccccgc gcccatctcc
    11701 atttggtgcc ccaaggaaaa agtggtgtac tgcgatcagg tggataccat gcaatacccc
    11761 caacccatag tgaccggcgg aagttactgg ttgtatggtc cctgggtttc caaatccctg
    11821 aacgaatgtg gagtcgggag catctgggtg gaatggaaga tcgtggatgg ttgcggaaaa
    11881 gcctatttct gcaaaattgg cgtttgggta aaaccaagga acacctatgc caaaatctgg
    11941 tggcccaagg attttgaagc ggacctctgc accgaccaga ccgatcccaa ataccttccc
    12001 tggccgtatg gctatcccaa agtcgaccag tataatccct gtgccaaata cgggatcagt
    12061 tataaagatg aagaatatac cttccccaac gataccggga tctgtaagaa aatcgttcgc
    12121 tggtggaccg taatagactg gtgtacatat aacccgaatt cctacgggta ttatactccc
    12181 ggaaaatgga cccatgccca gttgatcaag attgtaagcc gttccaaacc ggacatttcc
    12241 tgcgtcccag acattacggt tggccaggag acctatgccc aatccgcatg ggtggacatc
    12301 ccggtacccc aggttaccag ttcctgcagc ggcaaaacgg tgatctccca caactccata
    12361 tatgccactt cacccggggc cgatgcaagc gggatttacc cactcggcaa aaccaaggtt
    12421 atttttacgg tccgggacgc ttgcggcaac acggatacct gctccacgac ggttaccgtg
    12481 ctggatatga ccgccccaac cccatactgt atcggatcgc tgatcaccag cattgcctgg
    12541 catgccgacg gcgtttacac catcctggat ccgaaaaaat tcgatgtggg ctctttcgac
    12601 aacctgaccc ctaaacataa actgaaattc gaagccgtac caaaccggtt cacttgtgac
    12661 agcctgggtg cacgtcaggt gagaatttgg gtgactgatg aggcgggcaa caaggactat
    12721 tgtaccgtaa ccctgattct ccaggataac ctgggaatgt gtccgccccc ggtattgccc
    12781 gaagacacct tattcatcga aggatccttg ctcagcacag gggatgctcc cattgacagt
    12841 gtgaccatca cggtgtattc agacgatacg acagggagtc ttgctttgga tggtacttat
    12901 cgggcagaag gcctgacggc cggctccaac tacatcatta cccattcaaa atcagaccaa
    12961 ttcctcaaag gcgtgtctgc aagcgattat aagttgctga ccgatcacat tcagggctac
    13021 gatactcttg atcatcctta tcagctgctg gctgctgata tcaacgggga tgacaccatc
    13081 aatgtggaag atgcctacct gcttggttac tacctcttgt ccggaagtca ggtaccacct
    13141 tcacaggctt cctggaggtt cgttccatct gattatgatt tatccctcat ccaggtcctg
    13201 tccaattcaa ttgcgcaggt cccgcgtcac cgcatattta acccgctgaa cgcaagtgtt
    13261 tccggggctg attttactgg tgttaagatc ggtgaccttg attttagcct ccaggaagag
    13321 atctcagctc ttgaatttca atcgggcagt ccagccaaag cggaattcgt taacccgggt
    13381 tattgggtga aaaaacaaag gataaacgag gcggaggcaa gcggcaaaag ggcggcgact
    13441 ttggtaacgg taaaagagag caccagcctt tttccaaacc catttaatca gtttacttcg
    13501 cttcagttct ccagcccggc tgatgggcag ggtatcctga ggatcttcga ccttaacgga
    13561 aagctcatat attccagact ggaatcggta agtcctggtg tcaatctggt cagaattgaa
    13621 ggcagcaacc tcaggcaaaa ggggatttat atctataatt tgaccttgcc tggcatgaat
    13681 ttgaatggaa aactcacttt tatcgattga tttccattta cattagccca cccatttcgt
    13741 tataaagtat tgctataaag ttattaacat attaattata ttaataatat gttaaagcaa
    13801 tattaaatag ctaactattt aagggtattt tagcatttta tatattacat atttttgtaa
    13861 tgtttaatat tattaaaaac agttaaatat agttatcact attttttaat gtgttttcaa
    13921 ctattaaact cccatcccaa aattttgatc tgcttctgaa ggcggagttt taggcaaatg
    13981 cctctgctct gcctcctgca agctcatctc atttcaaaca gtatcgggtt gagttcctgt
    14041 cctcttcagg cgggccggcc tggaattcat tttgtcattg accaaaaatt gtttgatatg
    14101 aatcctatta tgaacttggg taaaggaatc ttcctggtgt tggcgctgct gaccctgcca
    14161 ttaaacacca tccattctca atgcttcctg gagaatggca catattatac cgcaccatgc
    14221 gatacaaacc tggcagactt tcttcatccc gacacttcgg atatcgggaa ctgccccaat
    14281 atcgtctcgg tggaagcgaa tctgcctttt cagaccatta tgaatggatg cgattccgcg
    14341 tgtttctccc tgctgatcag gcagtggagg gtgaatttca gttccggtcc ggatacgctc
    14401 ctggtggata ccatttgttt cacaccaatt tccctggcgg aggtattctg tcccagggat
    14461 accgtaatct actgcaacgg aggcctggcc gataccagtg tggcggccct gggctctcca
    14521 ctgcataaca acggaatccc ctgcaacatt ttggtcaacg gcccgaccca gatcctctgg
    14581 tcgagcactt ccctgggttg tctgaaattc acgaggcaat ggctggtcat ggactggtgc
    14641 acccttgaaa ttgacacctg caggcagacc atcgaggtcc gcgatacgac cgacccgtct
    14701 atttcagtca ttcccttgct tcaggatttc agcacctcgg gagatgcatg caccggcagc
    14761 gtgatctttc cagttgccac ggtgagcgac aattgcgcgg ccccgggcca gatttccgta
    14821 aagatcgagg tggcaggtca gaccctgaat tccaatggag ggattgtcca caatgtcccc
    14881 cagggcaccc accaggctgt ttatatagcg acggacggtt gcggcaactc cagtaccgat
    14941 accaccgtgg tatcggtgat cgataaggtc ggaccgatcg ccatttgtaa aagcgctaaa
    15001 agcatccagc tttcccagac cgggatcctg gtcatgccgg ccaccgcatt tgacaacgga
    15061 agttttgatc tgtgcgaccc tgaaataacc gttaaagtca aacggattcc gtcttcctca
    15121 ttctgtgcca tgccatacaa ccaggtgcac aagttcgacg atatggtcaa attctgctgc
    15181 atggatgcca ataaagacat tatggtcatt gtccgggtct accagggatt cattccggca
    15241 gggcccatca gcatcgatgc cttttccaat ccgtataccg aatgtatggt aatggtcagg
    15301 gtcctggata aagttggacc ggtgatcagt tgtccggccg ataccaccat agattgccgg
    15361 gatatccata agaaaagggt tcggagctta accgattacg gaacacccgt cgtatcggac
    15421 ctctgtctgg ataccgtatg gatcgacagc cttccccaac tcggggtttg taataccggg
    15481 gtcatcatcc ggagaatcaa tgcccgggac gatgccggca acgtttcgac ctgttcgcaa
    15541 aaaatatttg tcatcaatac ggatccattt gacggaaagg atacggccga tatcgcctgg
    15601 cccaaggata ccaccttttt tgtctgttcg gccaatacca gtcccgccaa tacaggtttt
    15661 ccggtgatca aagatgcttc ctgcagccag atcgtctttg gctcccagga tgaagttttt
    15721 caatatgtgc ccaacgcctg caggaaaata ctcagaaggt ggactgcgac cgactggtgc
    15781 cagctgtctc ctacggactc aaaggccggc aaatggtatt acacccagaa aatcattgtg
    15841 atggatacgc tgaaaccaat gattaccgtg ccggataccg tgaccgtgaa cagcatggac
    15901 tcgatctgcg gggctgtcca ggtctcggta cccctgccca gcgccacgga ttgcaccgac
    15961 tcggcttccc tgatctggtc tttcataacc gatattaacg gggatggaat tcacgacgga
    16021 caggggtatg gtaaagatgc cagccagacc ctgccggtgg gcaaacacaa aatcatattc
    16081 agcgtcaatg accattgcgg caacacctgc agcgacacca cggtggtcat cgtgagagat
    16141 gccaaagcgc ccacccccct ggttatgcat ggcctggcca cggacctgag cctgatgaat
    16201 ggagtggtca tggcgcgggt acctgcttcc ctgttttttg taccgggcag tgcggtggat
    16261 aattgtactc cctcggataa actgatcttt tccttctcac cggatacgag tgaccggttc
    16321 cggatattca cctgcgacag cctcgggatg cgaaccgtct acttgtatgt gacggacgaa
    16381 gcgggcaacc agggcgaggt gatctcctat atcctcatcc aagacaattt cgggtcctgt
    16441 cctggagcgg cctcgggcat caaaaccacc ggggtcggcg gaaagataca aactgaatgg
    16501 ggcagccatg tggagcaggt ggacatcaaa ctcatgcatg ggcaaacggt catgcagggt
    16561 gtgaacatga accagggcca atataaagcc aatggactgc aaatagggca atcctacgaa
    16621 atcataccta aaaagaatgt gaatccttac aatggagtga gtacgctcga cctgatcctg
    16681 atccaaaagc acattctggg actgcaaccg atccggtccc cgtataagca aattgccgcc
    16741 gacatcgata aaaacggtga aatcaacgga atcgacctgg tggaactgcg caaactgatc
    16801 ctgggtatcg ataaagcctt taccaagaat gaagcctgga gatttatcga tgccggttac
    16861 cgattccagg aaaaggcgga tccgctgaag cagcccttcc gggagcaata cctgatccgt
    16921 gacctgcaat ctcccatgca ggttgatttt atcggagtga aaatcgggga tatcaatgaa
    16981 tccagcattc cttccgaact gcttaatctt gaaagccggg aggcgacccc tgcactggcc
    17041 ttccatgcca ttgaccaaac ttattcctcc ggggatctcg taaggcttga attctttggc
    17101 gctgaacaaa cctcgatcaa cgggtttcaa ttgagcctgc atgccgcgga ccttgatttg
    17161 ctttccgtcg aaccgggttt gattccggca ggtcctgagt atgtgcacct ggaagggcat
    17221 acccttaaaa tgtcctatca tcaaagcaat gcccttcgtc cgaccgtcgg gatgccgctg
    17281 tttaccctgg tgttccgggc caggaatgac ggtagactca gccgcgccgt caacctcggt
    17341 tcagagccct ggctgcatgc ggaagtctat gatggtgccc tgaaagcctc accgttaaag
    17401 ctggattgga agggaaaggc aaatcagtcc ttcaatatgc aacttggcca gaatcgaccc
    17461 aatccatttt ttgagtcgac ggtcattgaa taccaactgc ctgagtcttc cccggtcacc
    17521 ctgaaagtca tggatctccg tggcaggacc cttctcctca gacaggaatc cggtacgcgc
    17581 ggacacaatt ccattaccct cagccgaaag gacctcggga ccggtggaat gtattattac
    17641 acggttgaat cggtttacgg gacccttacc cgaaaaatga tcctggccga ttgatcatca
    17701 attcttgatt ccctaaaaat tattattatg aaaataatta aggctaacct tcccatccca
    17761 aaaacgaaag ttagtttaat ccagatccgc agggtgctgt ccctgctcct cttcctgtct
    17821 cctttgctgg taagaagcat ggtccttcat cagggcaata cggaccattc caactattac
    17881 acccaaagcc cctggcaacc ggtctatctc ttggaagcct ccatggcggg tatgacttgt
    17941 tacacgcagt tgaacctttc cctgggggcc ggaggtaagg ccaaaatcct tccttccatg
    18001 atcctgaagc ccggtccgat caatgacgcg gatttcatgg taagaatata cggcacgagc
    18061 acagataccc tcagttgcct gcacaaggga cagaaagtaa aagtggaagt cactgaaatc
    18121 gccaccggca acaaatgctg gactttcatt ttggtcgagg ataaaatggc gccctccatt
    18181 caatgcaggg atacttcctt tctttgtgtc ggcgaccttt cctacctgga tagcattccc
    18241 ggactgtacg aggtggggga taattgcacg ccgctgcaga aacttaaaat cacccatatc
    18301 gataaggtcg agatgctgga atgcagcagc gatacttttt ccgtggtccg aaggacctgg
    18361 accgtcgtgg atccctacgg gtggtccggg agctgcacgc acaatattgc attgttgcgg
    18421 cctcagaaat ccgatattga ttttccgcag gatactaccc tttattgtcc cgacaccgat
    18481 gccagtcctt ccaataccgg ccagcccacc atcgggggcc agccgctgga taagttttgc
    18541 ggctggagtt tcaggtatga cgatgtggtc ttcaataaat gcggtcagac caaaaagatc
    18601 tggaggacct ggtcggtgct cgattgttgc gccttggagg ataccacggt gacccaatgg
    18661 atcctgatct ccgataccac cgaccctgtc atccaatgcg ccccggtcga cagtgtatcc
    18721 acccttgctg aatcctgtga agccaagtat atcattccgg agatcctttc cgctaccgac
    18781 agttgccatt cttccctcac caccattgta tgggtggaca gtcagtttat tgcggttccg
    18841 ggttcgatgg tcctccttga aatcgggacg catagcctgg aataccaggt gtccgatccc
    18901 tgcggcaata cctattcatg catgacctcc atcgtcgtgg aagacaccca tgctccggtc
    18961 ttttggtgca ccgacaccat tcaaatcggc ctgccggctg accgggtcat gatcggggcg
    19021 tccttttcgg aaatcgaagc ggtggataat tgcagcctgg ttggggtcca gatccgccgt
    19081 aagacggatt tttgcatgga tggttccgac gatacggttt ttggcgacag cgtcgccatc
    19141 tgctgcagtg atgtaaataa cccgttcgat attgtattta tcgccaccga catttatggc
    19201 aacgtggatt cttgcgtggt caccgcggtg gtggtggaca aaagtccgcc tgtcctgacc
    19261 tgcgaacagg ataccacgat actttgcgga gaaatcgtcc cggactggaa agatcccctg
    19321 gcaggcttga cggacaattg catggacagt atcgcgatca ctattgatac cctgatcaac
    19381 gatgtcaacc tatgcggtgt cggacagacc gtcaggcaga ttatagctac tgatctggcc
    19441 ggtaacaggg atacctgcat tcaacgcatt gcagtcagtg caggggatac cctgaccccg
    19501 gatgatatcg atctgggtac ggataccgta agggtgatgg gatgcaatct ggcgcttctg
    19561 ctcccggaca gcatcggata cgtaccaccg gtcgtggata ctccgttgaa tgactgccac
    19621 cggattttca taacctacat cgattcggtt cagccgttgg gcggtaccag cctttgccgg
    19681 atcacccacc gttcctggcg tgtgggcgat tcctgttaca gtgtttttcc cgtcgaggtt
    19741 ttggtccagg tcatcctgca ggacaccacc gcccctgctc ccctgcgggg cgggattacc
    19801 gggagggtga ccaatatagc agccattcca atagaaaaag tccatatcga agggaaaaat
    19861 atgcaggccc agcccgtatt tgaacaaatg accggagtgg atggccaatt ttcatcgggc
    19921 cggcccgatc ctgcgctaaa aatcaggctg gagaaagagg atcctgctgc attgaatgga
    19981 atcagcaccc tggacctgat taaaatccag aaacatatcc tgggcgaacg aaagcttgag
    20041 catcccttgc tggaatacgc agccgatgtt gaccggaacg gacaggtcaa cgtacttgac
    20101 ctgctcaccc tccgaaaaac tataataggc ctctccaatc cggatttacc ttggcatttt
    20161 gtgatccaat ccgctccgag cgcaaggata caacctggtt cctggctgcc ggaagagtac
    20221 atgttattca gtaaaccttc ggtcgaggaa tttattggtt tcaaaacagg agatgtgaac
    20281 atggatgcag tggtcaacca tacataccag ttggaaggcc gctcggctgc cgtcaccgaa
    20341 cttattcttg aagacagtga ggaagggctt ttaattaaag cagccaaaga tatgcggatg
    20401 ctgggaatgc aactgagcct ggtttctgaa gacggttggg ccgggaccca tctggtttca
    20461 ggaatcaaca aaccgcttga aggacattgg tcggtcataa ggaatgagtt gaagatcagt
    20521 tggaatgaaa accaggcgag ggtgttgaaa gcaggtacag tattgttcat tataagaggg
    20581 gcccattcca aaaacgtctt ctccggtact ttgccttcag agtggtatga tcatcaggaa
    20641 aaagccctgc cggttcgttt ggtgaaatcc gataaaaaaa cggatccggt caggatttcc
    20701 gcctttccca acccggtcag ccaacaattg aatatccgct tccccggaca ggggcccatc
    20761 caatcctgga gattggtgaa ccagaccgga aggatatcct tagagggcaa atcaccaggc
    20821 cataatgaat ggtcaataaa catgcagatc ctgccttccg ggctttattg gctcgaactc
    20881 attctgtcaa atggccaaat tgaaagacat aaagtattga agattaatta aaaaaacttt
    20941 agtaaaaagt ttaaattata ttaaattata atatattcgc attaataaat ttcattatat
    21001 atatgtaatt gaaatttatt ttgaccaaaa attcaaaaca atgaaaacta catccttctt
    21061 gtttaggcgg tctgcatggg tatttgcctt gttggttcag ctggcagtgg atagcgcttt
    21121 ttcaaatacc ctgtttgctc cggcgagata ccattattac cagataaatc catttgaggc
    21181 ctctgaggcc ctggggggca attgtcataa ggacaagcag cctccagtgg ctcaattgaa
    21241 aatgcttggc gagacggaat gtacatccct tcagggggcg ccctggtccg atttcctgat
    21301 cctttctgat aactgcattc cgaatgagca attatatgta acctataagg attccaccta
    21361 tctgaattgt gaggaacagg tgatcctcgt attcaggaag tggaaaatca aagacctggc
    21421 gggcaacaca accgtaattg accagatgat cccggtccag aaaattccgc tgaaacaaat
    21481 cgaactccct ttggacgtca aggtgtattg cccggagctg cccgatgcag cagcgcttgg
    21541 ggaggctgta cccacctata agggaaagcc cgtgtcccat ttttgcgggt ataccgtgga
    21601 ttacaaagac aaagctgccg tactatgcgg caattccaca gtgattacca ggcattggac
    21661 gatcaccgat tgctgcaact cctatgccat cgatcatgag cagcagattt attacctgga
    21721 tactacccgg ccggtcctta cctgtccggc ccctatggtg tttaaaacga atgtaaagga
    21781 atgttattcc catcagcgca ttcccggtat cgccgccacc gacggttgtt ccccgttggg
    21841 tattaaaatt caagtcgtag tcgataatgg gaaaacgtac aaggtaggtg acctggtcat
    21901 cctgtcggca ggcaatcata ctttcgaata caaggtgacg gatgcctgtg gcaacatgtc
    21961 cacgtgcacg gtcccggtca ccgtcgtcga cggccagggg ccattgctga gttgcaaggc
    22021 cattgaagtc tgcctgatca ctgattccat acgggtgggt ccggagcaac tggttgatga
    22081 atattgggac gattgccagg gattgaagaa ggtatccctt aaaatcagaa aatttgttga
    22141 tctatgcggg gatcccaagg acgacctggt ttttaaagat tccgtgacca tttgttgtgc
    22201 aggtggggaa gatgtagtcc gtgtggaaat tcaagctacc gatgccgcag gcaatgtttc
    22261 ctattgcagg tcggacatct atgttacgag taaactgaat gttgacattg aatgcctgga
    22321 tacggtcgaa gtagattgcg gggaaccgct tcccgaaatc ataccggaag tcattgcctg
    22381 cggggattac gacctggaca ccaaaattat ttttgacaac cgcgacgcct tcggcattgg
    22441 tacggtgatc atccggtata tagtgaccac ggcgggcgga atgcaagatt cctgtcaaac
    22501 cgtatttgaa gtgggtctgg aaggaaatcc gttcggaccc gaagacgtga tttgcccacc
    22561 cggaaccgtc accattgcag gttgcacttt ccctgacatc gcgggtatac ccgggatcag
    22621 tctgacggac accgcccggc cctgcagcca ggtgaatgtc aatctccaac tggatacgtt
    22681 tacagacctg ggaataccct gtttgcggat tacccggacc tggacggtga ccgatgtatt
    22741 gcaacccggc ctccaggtga tctgtgtgca aaacattgat atcatcgaca ccatcgctcc
    22801 ggtactgagc ggggccaggg acaccacggt ggtcgccgga ctcaactgta cggccatcgt
    22861 tgacctgcct gaagtcatcg ccacggattg cgataccggt atcgtcgtga cgaatacctt
    22921 caatgcacag gggggcaacc tggattctat tgttttcccg cttggacaga ccaggatcac
    22981 gttctggggg gtcgatgcct gcggcaacag ggattcctta agcatactca ttaccgtgat
    23041 agatacttcc ggtttcggaa ttgtttgcca gaacgacacc attgtaaact gcggtacggt
    23101 tttcgttccc agacctgccg tgatcgaggc ttcctgcaca caggtggcca ccaatgtatt
    23161 gacttcagac acggtgagca acaaatgcga catcactcat atccacttca aaagagtggt
    23221 tacggatacc ctgggcagga aggattcctg cacctttacc gtcactttcc gtgcatcgga
    23281 tacccttttc tgcaaccaga tccaatggcc taaagacacc ttgctggcca gctgcgggaa
    23341 gagcatacat ccggacagtc tgaatttgaa gcccatattt acatttaatg ccggttcctg
    23401 cgcccgtgtt gtagttacct ataaagacac cagcctcgcc ggtggaggag ggacctgcac
    23461 caacattaca cgtaggatat ggaccgtaag cgatacctgt gccatccctc ccgtcgtctg
    23521 ccgcgatacc cagctgattt ccgtcgtgga cacctccagg cccatcctcc aggtgcccaa
    23581 ggacacctgc gtatttcttg ctttcccggc cctttgcgat accttgctcg gtttcctggg
    23641 aaatgccacc gcgcaggatt gtgatcccgc cgtgatcata aaatccgtgg tcatcggcac
    23701 tacggacacc ggaggggctt ccttggtcag aagatatgga ctcggtgata cccgggtgct
    23761 ggtcattgcc caggacgcct gtggcaaccg ggccctggat accgtgttga tacaggtaaa
    23821 ggatacggtc aggcccgatg ccgtttgtgt caagtccaat aattatttca atgaccaggg
    23881 actggtgagg gtcaatgcca ggaccttcaa tggaggaagc accgacaatt gtactcctcc
    23941 ggctatgttg cgattttcct ggactaaaaa cctggcggat acgatcctcg aggttaactg
    24001 tgatgtgatc aaaatcatca gggcctccgg ggataccatt ctcgattcag tgatcgtcat
    24061 gccctttgaa cggaatttta atctctgggt cacggatgcc agcggcaatc aggatacctg
    24121 tgtgggcaac cgttttcttg ccttctttga taccctgaac ctatgcggaa agaatgccat
    24181 caaaaacctt tcttccatca ccgggaaaat ttccatggaa aacggactgg ccgttcccaa
    24241 ggtcattctc tcagccatcg gggatgagca atatcaacat ctttccgatg aacaggggac
    24301 ctatatgttt gataagatca atcccggcat atataagatg tttccgtata aaaacgacga
    24361 tacccggtcc ggaatcagca cggcagacct gatcgccatc caaagacata ttctcggcga
    24421 aaaaatactc aatccgaccc agcagatcgc ggcggatatc aataaggatg aggtcgtcac
    24481 caccctggat ttgattgaat tgcgcaaagt gattgtcggg atctatgagg tgttcccgca
    24541 aaatacttcc tggcgcttct ttgaccagtc tctgatggaa aaaatggacg atcattccgt
    24601 tatgaaggag atggaaaatc cattcgtgga agccgcggag aaagaaacca gccagctgaa
    24661 tttcatggct gtaaaaatcg gagatgtaaa cgggagcacc gttccacagt ttgcagccct
    24721 tgaaagccgg gaacaatcaa ccagatggct gaatatcccc gcctggaacc tgaacgaagg
    24781 ggaacaggtc gaattgccct tatccctgaa tcttgatgat ctggtcgccc tccaggccag
    24841 tattttcttt gaggacctgg atatcctggg tatcaaggag gagccgggag cgttgatcaa
    24901 accccacttc gtggccacca atcagctggg aaaagggtat tttaatttca gttggattaa
    24961 atccggaccc gctttcccgg cgcaagctac tcacctcgta agcctggttc tccgggcaag
    25021 gcgtgacctg gctttatccg aaaaggtcac cctggtacac aaatccctgg ctcctgaagc
    25081 ctatcaccag ggaggatccg tctccgctct tcaattgcga tttgaaccca tgcagaccgg
    25141 taaaatcctg gaccggggca ttgtactcaa tcaaaatatt cccaaccctt ttacgggaag
    25201 tacccggatc cgcttctcgc tgccggaagc cggggaaacc aagtggacct ttatggacat
    25261 taccggccgc gtgatccgga gctggagtct gcctttcgac cggggcagcc atgaaatggt
    25321 cgtggacaaa gcaggattgg gaacgtccgg cgtcctttat tacaggctgg atttcaaggg
    25381 atacacagag acccggaaga tgatccttct ggattaattt gcacagattt cattgtttta
    25441 tacaggcctt atccggataa ttccgggtag ggccttttta atatgcaata atccttttaa
    25501 ttgtaaaagt tttttattca gtatttaaca tatctagatt aatgtataat tataaaatat
    25561 tgatttataa ttataatata tattatataa ctatataata ttaaaaatac cacaaatagg
    25621 gtattaaccc gcatggatgg caagatatat ttgtgacata ttattatatt atgtaatatg
    25681 ataatacatg taagtcagag aatgaataaa aattagatat aatttgatca taatatctaa
    25741 tagtgctatc aaccttcttt ccaggtggac cacacccgga ctaacctttc tgagattgga
    25801 taattgattg tgtgcgccta tgctagcgag attccccatt gattgccggt atagcgctcc
    25861 cacagtttgg gctgcgaaag caacccaacc ccgattattt aatttaacta aaactaagat
    25921 ctcatggaga aaactacacg aaacagaaca agcctaaagg ctagaccggg attattccag
    25981 gccctaatgc tcatttttct ttttggttgg tcggccagtc aacagtcggc cattgcccag
    26041 ggttgcgccc tgacctgcca taatattaac ctctccttgg ataccgtgca gggaggggtt
    26101 tccgtaatcc ttccggagca tttgctttcc ggtgaggctt cctgcccagg gggaggattc
    26161 aaggtgacgg tttatgcacc gaacggcgcc ctcattggca actcggttga ttgcagttgg
    26221 gtgggctatt ccctgatcgg taaagtgacg gatactatca gcggcaacag ttgttgggcg
    26281 aagatcaatg tggaggacaa agcaggtcca aacctgaatt gtgtcaggga caccattcac
    26341 tgcctcgaag tggatgaata ttccctcggg gcggaccggt tcccttttgg ccttgctatt
    26401 gacaattgcg ggattaaatc acaggtttcc atttccatgg tatgcaccga atatccctgt
    26461 tccaatacac aatttgtagg cctgtgcata aggacggtca ttgccaccga caagtgggga
    26521 ctgaccagca gctgccagga cagcctctgg gtatccaggg attccctgga caatttaatg
    26581 ggggtccgtg acacggtgct ggactgcgcc atcgcggaca ccatggccaa agatgccaat
    26641 ggcaacccga tccctcaaag ggtgggtacc cctaccatct cgggcatccc cgtatggccc
    26701 aataacaacg tttgtaagat cctgagtaaa tataccgacg tggtctatcc gatttgcgga
    26761 agtaaaagca gaaaaatccg gcgggaatgg accatcgtgg attggtgtgc cggcgaggat
    26821 acggtattga tccaatggat taaaattgtg gacaccacaa aaccggtcct ggtcgaccgg
    26881 ggtgaaatgt ccgcttatgt aggacctcac aattgccagg ctcccgtgga ccttacaagt
    26941 cccggagcca cagattgctc cggcatcaaa gaagtttttt actccgcggt ggtcctttca
    27001 gacggacacg gaggattggg agcggtttac accggatata tcaacggggg cagcaaacgt
    27061 atctacctcc cctccggcag gcatgaagta cactttttcg tcaccgatca ttgcggcaat
    27121 acggccctgg ataccctgat cataaacgtc acggatcatg tccccccgac cccggtctgt
    27181 gacgaacaca ccgccactac cctggatccg gtaagttgct gggcgaggat ctatgcatcc
    27241 gacctggaca acggcagccg ggataactgt tgcggtaccc tctacttcgc agtggcccat
    27301 atggattcca tcactgctta taccgaactc ctccgcggta aaatcatctc caaatacaaa
    27361 gaatcggttt accacagcaa ccgggacttc tttgaacagg tgattgaccg gtggatcaat
    27421 tgccatgtct ttgaagaata tatcgatctc ggttattgcg gggttaacca actcgtgctg
    27481 agggtatacg aagcctgcct catacccaag tacgatccgc atgtgcattc cgattcaagg
    27541 cagtcgtttt actgcaagga agcctacaat ggacatgatg gggcctacaa tcaggcccag
    27601 gataatgtgg acgccctgat cgactatgtc ctgaaaggca ttggaatgaa agtccagccg
    27661 aatttcccgc tgggatatcg aaattacagc gactgcatga tatttgtaaa cgtagccgat
    27721 aaggtggctc cgacctgcga agtggatgct gaaaaaaagg tctactgcga cggggttcct
    27781 tatgtggaac tggcctggaa ttacggcggt ggtaaagatt tgctgaactg ctacggcgca
    27841 aatggaatct acagtcccga ttaccttgaa ctggatcaat acgacaacgg agggaaaatc
    27901 aatccttacg aatactttga taatccggtt tttgaagaca attgtggaac ggtcaaggtg
    27961 gataccacca ccaccggctc tataaacaat tgcggagccg gtgtcctgac ccgttcctgg
    28021 accggtacgg atgaatgcgg cctgctgtcc accacctgcg cacaaaaact aaaagtattg
    28081 caccgctcgg attttgaagt actgtttcca aaggatatag aaaccgtctg cctcgacaac
    28141 ccggcccagt ttaataatcc caccggtgaa aattatccga aaattttcga cgatgattgc
    28201 gaacacatcg gcattacctt ctcggaccag cggtttgaca tcgtagccga tgcctgctac
    28261 aaaatcatcc gcacctggaa ggtgatcgac tggtgtgcct acgatccgga ccagcattac
    28321 cgcacaaagg attatatcgt ggatacggcc accgaatacc gggccgtccc ggataaatac
    28381 caggaccgtt tctgtcagtt caggtacctt aaagacaata acgacggtta tatcctgtat
    28441 acccagatca taaaagtcaa aaacaccgta gccccgacca tcgccgtgga ggacagcgtc
    28501 gtcacctgcg ccggcagcgt cgaaacctgc ctgggccata tcaaacttaa agtaaacact
    28561 acggatgatt gtacccccgg tcctgaaatg aggtgggtgg tctatattga ccgtgggaat
    28621 gacggatcca cagaacagac ctcttatcac agcggttcca cggtcacggt ggactcggat
    28681 ttcgaggtag gacagcataa aatttctttc ctggtcgagg acatctgtgg caatgaaacc
    28741 agaaaagact ttgtgatcga tgtggaattg tgcaaacttc ccactccgta cctgctcaac
    28801 gggcttgcag tggacctgat gccgatcgac aacaataatg atggtaatac ggaatccgga
    28861 atggtaagga tctgggccag tgattttgat gccggaagca cagccggctg cgggcagcag
    28921 gtggtcgctt tctccttctc cagcgatacc tctgacaaat ccaggaccta tacctgtgac
    28981 agcataggcc aaaggtttgt gaacatatgg gttacggaca gttacggaaa ccaggatttc
    29041 gcctcaacct atatcctgat ccagaacaat gccaatgcct gcgccccggg tacccaggcc
    29101 atcaccggca cgatcacagg ccggatcaaa accgaggatc agctcgatgt tgaaaaagtg
    29161 agtgttcaat tgcagggcgc caacagcctg cctgcggtga ccaaggcgga tggcttgtat
    29221 gccttcagca gcatgaagat gggaggatcc tataaagtga tgcccaaaaa ggacatcaat
    29281 ccgatgaacg gagtctccac cctggacttg ttgttgattc aaaaacacat cctgggcctg
    29341 cagccgctga actctcccta taaactgatc gcagctgaca tcaataaatc gaacgagatc
    29401 agctctgtgg acctggtgga attgcgcaaa ctgatcctgg gcatctacga caaattccag
    29461 ggtaatgaat cctggaggtt tattcccaag tcacacacat tcaagaaccc ggccaatccg
    29521 tttgaaagcg ggtttcctga ataccaggat atttcgagcc tgggccataa ttcttttgcc
    29581 gatttcgtcg gagtgaaagt gggcgatatc aatggcactg tagtcgccaa ccagttgttg
    29641 ggaagcgaat cccgtgctcc cggaaaagaa ttccttctcc gggccgatga cctctctttt
    29701 aatgaaagag agcaaatcct ggtgccggtg tatgctgccg atttacaact gattcagggc
    29761 taccagttta ctttgagttt cgatccaaaa gtcctgaagt taagccaggt cattcccggc
    29821 gcaaagggca tgagccaggt caatttcgga ttgcagcggg cagttgaagg ccggattacc
    29881 accagttgga cgaccacgcg cgctttggaa tcgatggatc aacccttgtt taccctggct
    29941 ttcaatacgc tccggaaggg caggctccat gatcagctgt acatcaattc gagcgtaacg
    30001 gtagcagaag cttacgacag ccagcacgat atcctgaacg tccggctgca ggtgggttcc
    30061 aaagatggaa agggtgaatt tgcccttctc caaaataccc cgaacccatt cctgcattcc
    30121 acccggatcg gatatcaaat gcctgaaagc gggcaggggc tgatgaaaat ttttgacctg
    30181 aatggcaggc tcgtgaagga gatcaacttg aaataccaga agggatacaa tgaaatccgc
    30241 atgagccgca acgaactgaa atcatccggg gtcctgtatt atcaattgca ggccgggaac
    30301 ttcaccgcaa cgaaaaaaat ggtggtcata gagtaaacca tttttaaatc atactgtttt
    30361 tgggatggga cccctctttg ccaaagggca aagggggtct ttttttttat ggtttgcccg
    30421 tggtgaaatg gaatttttga gcctaaatcc gggttatttt tgcacgatca atggccaggg
    30481 cgcttggaat agatttcgga tttaaaagga ccggtctggc ggtgacagac cctcttcgca
    30541 tctgtgtcaa ccggctggaa accatcccca cggaaacctt cgaccaattc ctggaggatt
    30601 acctgaaaag ggaggaggtg gacctgatcg tgtttggaga gcctttgcac agggatggca
    30661 cggccacagc ggtaagcatg gaggtgcacg aaaaggcaaa gaaacttaaa ataaaatacc
    30721 cttccctgaa gattgatttt caggatgaag aaggaaccag cagaaaagcg gtccgcatgc
    30781 tcatagacaa gggaacccct aaatcgaagc ggaacaagac gtccattgac cagatgagcg
    30841 cagtgttgat tttacaggaa tatttaaaac actactaaaa tgattttgcc catatatgcc
    30901 tacggccagc cggtgttgaa gcaaaccgga gaagaacttg attttgaccg gtttaccggt
    30961 ctggaggaat tgatctccaa tatgtgggat accatgtatg cagccaaggg ggtgggattg
    31021 gccgctcccc aggtcgggct gagcctcaag ctgtttgtag tggataccac tccttatgag
    31081 gaggagggta aaccctttac cggcattaag aaggtattca ttaatccggt caaactggat
    31141 gaacacgggg aagcctggtc ttttgaggaa ggttgcctga gtatccctga cctgcgcgca
    31201 gaggttgacc ggccggaacg ggtaaacctc tcctatttcg atgaaacggg aacggtcagg
    31261 gaagagctct ttgacgggtt gaatgcccgg gtgatccagc atgaatatga tcacattcag
    31321 ggcattctct tcatagacct gctaaaaccc ctaaaaaaac gaatgttgca aagacggctg
    31381 gaagccatta aaaaaggcca ggtggaagca gattacaagc tgcgctttta ccgcaaaccc
    31441 tgactgctcc tcaattccag gcttctttgt atatatttac ccaaaaggtc aaactcggca
    31501 ttcacccttc gaccggcatg gataaactgg aagttggtat gttcataagt ataagggatg
    31561 atggagacct caaaactatc gtttaaaaga ccggaaatcg tcaggcttac accgtttaat
    31621 gcaatggaac ccttgggaat caccaatgat ttgaatgggc tgggaagggt aaactcaaaa
    31681 acccagctcc cgcgccggtc tgaacgggcc aggcaggtca gggtggtatc cgtatgaccc
    31741 tggacaaaat gaccgtccat ccgggagtca aaacggatgg aacgttccag attaatctgt
    31801 tcaccctgcc gggcatccga aaaatgggtt ttatccaggg tctcccggat cagggtacag
    31861 cggtgccatc cgggacccag ctcatccacc gtaagacata caccatcatg ggcaacactc
    31921 tggtcaattt ttaattcatt ggaaatcggg gattccacaa agacctgaat attggattta
    31981 tcgggcctca gaccgatgat cttgcctgag gcctctatga tacccgtaaa catatcacct
    32041 gacgatttgg ataaatcctt tccggtcttc aatgacctgg gtggtgttct cctgaaagcc
    32101 gaataccctg cagatataat agtaggttcc ttcactcagg ttccttcctt ttttatcctg
    32161 cccgttccac aggatctggg gatcctcggt ttcaaacagg atttcgcccc atcggttgta
    32221 aatctgaaat tcaatcttca gcacccctgc atttatcctt ggaacaaaaa gatcgttgga
    32281 cccatcgcca ttcggggtga aagtattggc cagttcgtat tcaaggttgc agttagccgg
    32341 acagacttca ccggtcaacc tgctctcatt gtcgttgaaa tccacggtgg tgatgacgta
    32401 gcatccggta aaaccggaat cgggaatgtg cttgaaagtc gtagcggatg catcggtaag
    32461 ggtagcgatc aacctgaaag cccctgcgga tttattgttc ttaaaataaa tgttgaaagc
    32521 cttaaggtcg tcgataaaac aggtatcggt attgctaaaa ccccagacca gttcattggc
    32581 aatactgcca tccggttcct tcgtgacgca attacggttg atcgaaagat ccgggctgca
    32641 gggggggacc gtatccaatg gtgtggcaca ggtttcctgc gaaaaattga acattgggct
    32701 gggcaggcca ttgattccat aagttccaat ggaccggatc cgataacaat aagtcgaatt
    32761 gttactcacc gtcttgtccc ggaaaaaggg ggtggttgaa cttccaacca attcaaatgc
    32821 gcctgagccg gaccttcggt ggacttcaaa attgtagttg ttccacggaa cctgggcttc
    32881 ccaggagagc agcacctggc ggtccgaagc gatggttttg gcatataccg tggatgccgg
    32941 gaaagaagat ccgtaagggg taggggaatt accggtaaaa aactggatca gataggaata
    33001 gggtttttct gaagtggaga ggttccggtc caaaaagaca gtatccgtaa aggttttaag
    33061 aaattcggag gtaaactccg ccccggggat tggagtgaaa accgtattgg tattgaaaat
    33121 cccatccgcc ctcagtagga cgagtttata aggccctttg tttttgaggg tatccaggtc
    33181 gtccgcgagc ggcatggccc aatggatctc tatctctcca agagtcgggt ctgtagtgtt
    33241 gaccgtagct ttggtaataa agggaaggtc cctcttgagc tggacacaga tctcttcaga
    33301 aggaaggctt gcgacccggt tgtatgaatt gcctccgggc gaggtcagtg caaattcagc
    33361 caggattctg taacaatgcg ttttgcccga ttccaccatg tcgtcttcaa aataatacgt
    33421 gtcctggtca cggctcagta cattggaagc gatccgggta taccctcttc cggcaaggcc
    33481 gggtgtacag gaatcgatga ggaattggct gctgttgatc ttcctccata ccgaaaaccc
    33541 tttaaaatag tcgtttaccg tcacggcaca agcattcggg taagtccagt tgatccggat
    33601 aaggttgttg ttggctgtgg atgaactcac attctccggt gggggcccga tgactttgat
    33661 tcttaccgtt ttaaggtcag cgagacctga agagtcctta aaattatcaa tggctctgaa
    33721 caccacagtg aaaggaatat cagaaatgtg ttcgcaagag gtctgccaac tgaaagttcc
    33781 cctggcaatt tggttctgga aaccggaagg ggcgtcgaaa acggctttat ttacagatac
    33841 attaaaaggg ccgccaaggg caaaaatggc aattttctga ctcaggttat tgtcggaagc
    33901 ctgaacctgg aactcgacct tcgaaccggc aataatgcac aggtcattgg gcacggtcag
    33961 ggaaggaggt tcattgctgc aattctcaat ggtgatctgc atatcccgga tgatggtatt
    34021 gagcaggatc ccattccggt attccttgat caggatggcg atgttgtatt ctccttcccg
    34081 ctgtggcgca atccattcaa aattgcccgt agtccggtcc agggatattt tattgtccgc
    34141 tccgggtacg atctggtccg ggaaacggta attcgggact tccgaaccgg tagcctggaa
    34201 agggacaatc agttcgtatg ccagggaatc cccatccggg tcataggcgt tcaggttgtg
    34261 gacgaatttc cggtcgatgc agccaaaatc aatcgggggt tgtaaaagaa ccgctgaatt
    34321 attgtatccc tgaatcgttg aattgagcag ggtgagggtg gtggcgatgt ggaactggat
    34381 gcctacggaa ttgggataat taagattgag gatatttccc acccggtttg ggtcggtcat
    34441 ggtcaccgta taagtacctg tgcccgggta agtatgttcg gcgacgtaaa aattcctttt
    34501 ggtatcgttt tgcaggatct cccctttccc caaagggccg ttgatccggt aaacggcctg
    34561 cgagtggcca tcaccccaga ccacggtaac gctgtcccgg tccgcaccgg tactgctggc
    34621 tttggtatag gtggtcacgg taaggcgaat cgtaaggggg cctatctgga cataagtgat
    34681 atcccctgcg cggttgtggg tggcgctggc tatccctgca taacaggtca gaaagaggaa
    34741 aagcgaaatg ctccggaaaa tcttcatata aaatataaac gccgcgggcc tcaaattggt
    34801 caccgttaac aaaaataccc gttttttttc ttgcttttca tctggggctt gcaggaaaag
    34861 ttcatgcatt gcgcagcgat ctttggggat ccggggcccg gccgctccct gtcaggaatt
    34921 acccatacaa tccatgaggg cggttttcca atcccggatg gatagttgaa aggttgattt
    34981 gatcttgttc agattaaata cactgtaaga gggcctggcc gcaggccgtg gaaagactga
    35041 ggtggaaaca ggctggactg aacaggtatt gctgcttccg gaaacgatac ctgaggcgaa
    35101 atcaaaccaa ctggcttcac cctcattggc gaaattgaaa atgccatacc aggaggggtt
    35161 tcctgcctcc gacaagacct tgctcagaat ggtcaaggtg gccgctgcca tgtcagggac
    35221 ataagtgggg gcgccgatct ggtcattgac cacttccagg acaggtttct cggaggaaag
    35281 ccgttttatg gtattaaaaa aattatggcc ctcccggcca tataaccatg aagtccggat
    35341 aactagggta tgcggatgat ggtagagtgt tttctgttcg ccggcaagtt ttgatgcccc
    35401 atacacgtta cggggattgc agggatcggt ttccaggagc ggccggcgaa gcgcattgtg
    35461 atagacatag tcggtggaaa aatgaatcag gggaatttgt tttttcctgc aggcaatggc
    35521 gacctgcctt ggtccttctg catttaccag ccaggcccgg tcaggggagg tttcggccag
    35581 gtcaaccgaa gtataggctg ccgtgttcaa tacaagatga atccggtggg tatccaggca
    35641 atccatgagg ctgtccgcat cggtgatatc cagtctggta tgggacaggg ccacggggtt
    35701 aaaggcgtcc tgactgccca acaattggtt caaggcatga cccagttgtc ccccagcccc
    35761 ggtgatgaga atgttataca cggggaatca ggttttcagg tccttcaata aaggcaaccg
    35821 gaggtccttg gtggatattt ctattggaac cccgggatca ggccattcga tccccactgc
    35881 ggggtcatta tagcgcagtc ccgattccga tgatgggtgg taatattcat cgcatttata
    35941 ttggaagata gccgaagcgc ttaaaaccag gaaaccatgt gcaaatccac ggggaatgaa
    36001 aaactggagt tggttggcat cattcagttc cacaccgaaa taccggccaa aggttaaaga
    36061 acccggtctc agatccacgg caacatcata taccgctccg gaggtcaccc ggaccagttt
    36121 ggcctgcgcg tgcggcggat tttgaaaatg aagtccgcgc aacacccctt tttttgaaaa
    36181 ggactggttg tcctgtacaa aatcggcctc gattccagca gcccggtaat ccttatcgga
    36241 ataagactca taaaaataac cacgatggtc ggcaaaaatg gagggcctga tcagcaacag
    36301 tccgtcgagg gggcagggtt cgaccactaa agccatcttt atttttcttt aaaaaaaatg
    36361 gaaatgggga ttcccgaaag cgggtaacgc tcccgcaact gattttccag gtattgcctg
    36421 tagttgtttt gtatataatc cgggttgttg caaaaaaagg caaaggccgg atagggcttg
    36481 ggtacctgcg tgacgtattt aattttcacc agccttccct tgtaagcagg ggggggattt
    36541 ttggcgttga tttccgccag gaattcgttc aggtccgacg tctttatttt ctgtttcctt
    36601 ctctcataaa cgtccatcgc gctttctacg acctgaaaaa ttctttgttt gttttgcgca
    36661 gatatgaaca ggatgggcag atcgttgaaa ggggcgagct tgtgttttaa tgctttttcg
    36721 tactggtgga gggtattggt atctttttcc accaaatccc atttattgac caggatcagg
    36781 atgcctttat gttttttgat ggcaagtctg aacaagttgg aatcctgcgc ttcgattcca
    36841 tgacgggcat cgatcataag gagacagaca tcacattcct ccagtgattt gatcgcccgg
    36901 atgacggaat aaaattccag gtcttcatgc accttcgtct tccggcggat accggccgta
    36961 tccaccaata gcaactggtg accgaattta ttgtacagag tatggatcga atcccgggtg
    37021 gtcccgggga tatcggtgac aatatttcgt tcttccccaa gaagtgcgtt cagcagcgtg
    37081 gacttgccca cattgggttg tccgatgatg gcgatctgcg gcaactggga ttgctccttt
    37141 tctttgtcct ccggtgcgaa aagttcaacc acgtcatcca gcagttcacc cacccccgat
    37201 ccgctgatcg aggaaaggaa ataagtgttg tcgaacccaa ggccataaaa ctcattggcc
    37261 atcaacatcc gctggtggtt gtcgacttta ttgaccgcaa ggtaaaccgg cttgttgctc
    37321 ctgcgcaaca gattggccac ctgctcatcc aggtcggtaa tgcccgtggt gacatcaacc
    37381 ataaaaatga tggcgtttgc ttcatccacc gcaaaacgga cttgctgccg gactgcagct
    37441 tcaaaaacat cgtcggaatg ctgaacgaat ccgccggtat cgatgatggt gaaattcttc
    37501 ccgttccact cacattctcc gtagatccgg tcccgggtca ctccgctggt atcatcgatg
    37561 atggcctgac gttccccgaa aagtcggttg aacagagtgg atttacccac atttggcctt
    37621 cccacaatcg ctacaatgtt gctcataatt ttttcaataa ccgtattttt tcagcgcttg
    37681 gggattattc ctccagtttt tttcaacctt tacctggaga tcgagataaa ccccatgacc
    37741 caggaaggat tcgatttcct ggcgggcccg gatgcccaga tctttgatgg cctggccttt
    37801 ctggcccagg agaatagctt tttggctctc ccgctccaca atgatataga caaaaatccg
    37861 ggcgaagggc gacccttcct tgccggtggt ttctttatag tcttgcacct gaacttcaca
    37921 ggagtaaggg atttcgtcat gaaacagccg gaaaatattt tcccggacga tttccgcaac
    37981 aaagaaccgt tcatgacggt cactcaggtc ttctttggga taatagggcg gtccttccgg
    38041 caaggcttcc aacagggagg acatcagttc ggggatattt tccagggtga gcgcagaaac
    38101 gataaatgat ttttggaacg gatggaccgc agcccatttg gccacgtact cagccagcct
    38161 ggctttgcca aaaagatcgg ctttgttgac aaccagccaa acgggcattt ttgccctgga
    38221 aagggtagcg gcgatctccg gatcaacggg ttctgctgcg gtggtgcaga aaagcaaaat
    38281 atcggcatcg tgaagcccgg catgggcctg caggttcatc ctctcctgca acatgtattg
    38341 cggcattttg attaggccag gggcatccga aaaaacaacc tgatggccgg ggtcattgta
    38401 aatgatgaaa atccggtgcc ttgtggtttg tggccggtca ctgatgatcg ccagtttctg
    38461 accgcagaac gctttaacca ggctggattt cccaacatta gggttaccca gtaaatgcac
    38521 aaatccggac cggtgtgttt tttccattta aaggtgcaaa gatactcatt cgccggcatg
    38581 gaccggggcc ccggtccggg aaccccttga gggatgaaaa ataggactca aaaccatgac
    38641 aatcagaata ttatttatcc ctgagtacct gatttttttc agcagacccc ggtcggcgga
    38701 agaggctcca taaagacaac cgcttcatcc gggctcagta cctcagtcgc ctggactggc
    38761 tatgcaaggt cttttccacg atggcaaata caccgaccgg atcaagccgc ctccactcga
    38821 tggcattaaa attcttgccc aggtggatat aatgctggag gccagcctgc accatttctt
    38881 cctcgacgtg ttcgagcgtg ccgagcatga cgatccatcc tccttcttcc gacagttctt
    38941 cctgccattc ctcgtaataa caatcgtccg aactgtgaaa gttgtataca ttgtccatga
    39001 tgagaatgaa gtatttgatt tcggaccgga ggagtttttc gatcacgttc cttttcaggt
    39061 acatgatgtc gttttgtatc gcatcattcc actccccaat gagttcgatg atggcgaatt
    39121 gctcagcata atcgacgaaa aggatcttga ggtacaaggt actggaatca aattcatccc
    39181 attggggatg gatgtaataa ttgtagatgg catgggtata ttcaaattcg ttgtattgcc
    39241 gcctgtaaaa cggcgaatgc gggtcttcgg aagccacata ttggtctcgc catctgaaaa
    39301 aaggttctat atcttgcata ccggctctaa ctaggatggt aatttaaagg attgtatcat
    39361 tacaaagttt aaatttcagg caaattaacc gcatttttaa gatcaacatc accggaccgg
    39421 aatcagctgg aaaatccaca ttgagcgctt tcctggccct gcaataccaa gacccttggg
    39481 tcgaagaata tgcccgtgta tttctgaacc agggtcaggg gatccgggat gcaggcaccc
    39541 tggtcagcct ggcccacatc cagattgaat gggaaaacct ggctttaaaa gatgcggacc
    39601 gggtcgtctt ctgcgacacc gaccccctgg tattattgat ctgggcccat gaaaaattca
    39661 gaaagcggcc ccctgagttg gtggccctgg ccagacacca ccgctatgac cttaccctgc
    39721 tttgcaaacc cgacttggac tgggaaccgg atcctttgag ggaaaatccc catgaccggg
    39781 acaaattgtt tgacctttat ctggatcatg tgaataaatt ccggtttaac tatgccatcg
    39841 ttgaaggatt gggggaagca cgcctgaaaa aggccctggc cactgttgat ccttttctga
    39901 aagcacatta actttgttcc atctcacgat atcggggtgc ctactgattg gattaaatca
    39961 tggctgagat tatacccgtt gaacctgtcc gggtaatact ggaaaggaaa cttaaaagct
    40021 ccgatcacaa actgttttaa acgttgatcc atgagcttaa aaatattttc attgaccctg
    40081 gttctgttgt tcacaaggcc cctgcaagga caggaaataa tcctgcaggt cctcggtccc
    40141 gacgacccgg tcaacggagt attggcttat tcccacgacc gctcccggtt cgccatctct
    40201 gaccgggagg gcaagatgaa ctggaaactt gttgcttttc cagataccat ccatatttca
    40261 cacctctcct ttttggacca tactgaaatc attcaaactg ctgcccttgc cggcgggacg
    40321 ctcctaatca ggctccaacc cagaaccata aacctggcca ccgctgaaat cgcaaccacc
    40381 tgggttaaac ccaataccca tgtggtacag tccaccctta acgagagcca gattgccagc
    40441 aggttcctgg tgcaggacat tccttatatc atccaacagt ttccttccac ggtgactacc
    40501 agcgacgcag gcaacgggat cggatatacc aatgtccgga tccggggcct cgaccccacg
    40561 caagtcaatg tcatggtcaa cggagtgccg ctcaacgatg cggaatcaca ggccgttttc
    40621 tgggtggacc tgcccgacct gatcgcttcg gcttccgctc ttcaggtgca gcgggggata
    40681 ggcacttccg gggccggcca ggttgctttt ggatcatccc tgctcctcag cacctacaaa
    40741 acgtcccccg tgccctatgc ccaactggag tcctccgtgg ggtcttttgc aactttaaaa
    40801 aacagtctgc aactcaattc cggtaccttg ggcaaggcct ggaatttttc cggtcgagtc
    40861 tcccggatca ggtccgatgg atatgtggac cgcgcggctt cttccctgtg gtcaggtgct
    40921 ttcaacgcct cctatatcgg gacaggacgg tctgtacggt ttttcatctt tgatggcttc
    40981 gaaaggactt accaggcctg gtatggattg ccggtgcaat atgttggcga caaagaaaaa
    41041 aggacctaca atcccgcggg aaccgagaag cccggcctgc cctacgagga ccagatcgac
    41101 cgttaccggc agacccattt tcagctcatt catcaggaag atcagggtaa acgatgggct
    41161 tttcagaaca cctttcattt cacaccgggc aggggatttt atgaagaata caagtcaggc
    41221 cagtctccct cggattacct gctggacagc ggggatgaac tcgatattgt ccgcaggcga
    41281 aatctcgaga atcatttcct gggctccatt cacacggcca ggttccggat gggggattat
    41341 gaaatcaatg ggggtttggc atggaacctg tattcggggc gccattttgg ccaggtcatc
    41401 caggccggaa acaaaccctt gcctgaccag ccggtctatt acgacaacga ggcaaccaaa
    41461 tggtcggcga tgactttcgg aaaaatgacc tgggacctcc atccctggtc cctgatggca
    41521 gaccttcagt accggaggat tgggtatagc tatcagcccg cctatgaccc tgaacaggga
    41581 attaaaacag tacatcatca ttttttaaat cccaaacttg gatttaccct tgccgttggc
    41641 ccccatgccc agcttttcgg tttctccggt atagcccggc gtgaacccaa ccgggatgat
    41701 tatgtggatg cggaccggac gcttccgagg gcagagaggc tttgggatca tgaagccggt
    41761 ttccgctggg gcagaagaaa cctgcagatc gaacaaaata tttattacat gatgtacaaa
    41821 gaccaactga tcccgaccgg aaggctgaat gacgtaggcg cgtatatccg cagcaacgtg
    41881 gaaaagtcct accgcctggg gtctgaaacc tcagtgacct ggaatccggg aaaattgaat
    41941 gtacaggcca gtctgaccct ctcccggaac cgtaccctga attatcaggc ttatttcgac
    42001 gactgggatt ccggtacaca ggaaaaggtg aattatggaa gccagcccat cgctttttct
    42061 ccctccttta ttggaaatct gtcttcggaa tacctttttt atgataaaca ggccgggcag
    42121 acccgtcatt ttttattcct ggatggctcg gtacaggcgg taggtcgtca attcctggat
    42181 ggtaccgggg acccaagcgc cttgctgccg gcttaccgcc atgcccaact ggctttgcgg
    42241 taccgcctga tcggtgacaa aattccgtca atggacttcc atttacaggt caacaatctt
    42301 tttaacagcc gctacgagtc caacggatgg atttacaggt tcaggtcgcc ttcatacaac
    42361 ccggtaccgg atgatcctta tgcgggtggg gaaggggacc accgctatta tctgaagggg
    42421 ctttatcccc aggccggccg tcattttatg ttatcctgcc ggatccgatt ggaaggaaag
    42481 ggcggttaaa aattgtaggt gatcccaaaa cggtaaacca ggggcagggt ggtgctttga
    42541 ctgtctgaag tcaggtcata aaggaggtag atctcggatt tgaggagtcc cccgcctgta
    42601 taacccaatc cgaccagaaa ctggttgtcc ccaactcttt ccgtttccag ccggttgcca
    42661 ttaaatccca ccggaaagac cctgttgata taactgaatt cggtatgcgc aaaaagcaca
    42721 ttcaggaatt ttgcgcggcc gaaaagcccg gcgccatagt caagtccatt gtatttgtag
    42781 accgggcccc caaaatttct gaaccggccg gtggtatata aaaactcaaa acgggggcct
    42841 gcggataaaa aggaattgaa tttgtatccg agcatgggag agaggccgat gccaaaggtg
    42901 ttgccgtcca aaccggccag gccacttgaa aaattgatgg taaaacctcc cccgtaccaa
    42961 agatggtcgg caaaggattt catttccggg gattgttctt tctgaggctg gcgtcggtaa
    43021 atctgggctt ccagcctgga tggactcaaa cacagaacca gcatcaggat ggagaacacg
    43081 cggaggcagg tatatttcat aatcggagga tgttgattag ttttcaaaat taggctttcc
    43141 ggccagagtc tcttccaccc aggacagcgc gagttcaaat tgttgttttt cattgagaaa
    43201 agaattgtcc aggacccggg cctccggcgc ttttttcaaa ggcgaatcgg accgggtgga
    43261 atcgatatgg tccctttcga gcaggttttg tttaatcact tccttgtcca gattcagccc
    43321 tttcaggaga agttctttaa accttcgttc cgtccggatt tccaggtccg cggtcatgaa
    43381 tattttaagt tcggcatccg gcaaaaccac cgtgccgata tcgcggccat ccatgaccac
    43441 gcctttgtgg atagccattt gtttttgctg ccgggaaagg aaccggcgaa cctgggggat
    43501 cgtgctgacc ggactcacta agtcctggat ctcaggcatc cggatcgctg cggtgacatc
    43561 ctccccgttc aggtaaatct tgtttcccga ttccgaccag acacaatcaa tttgaatatg
    43621 gtccaggtgg ggcaggaggc ttgccggtaa atccccggtg atcttatgcc ggatggcata
    43681 gagggtgacg gcccggtaca tggccccgct gtcgatgtac ttgtatttga ggtgacgggc
    43741 cagggcctgg gcgagggtgc ttttaccaca agcggaataa ccgtctatgg caatatttat
    43801 ttttttcggg accgccatcc tattttaatt tgaagaggct ataccgggtc gccgatatag
    43861 cctcttgtat tttttcggtg gtactggaaa taaggatcaa tattcatcct cattgaacag
    43921 aaaatcatcc ttgcgcgggt agtcgggcca gatatcttcg atgttgtcgt agatttcgcc
    43981 ttcatcttcc atttgctgta aattttctat gacttccagg ggagcacccg aacgaatggc
    44041 gtaatcgatc aattcatcac gggttgccgg ccagggagcg tcttccaggt ggtgagcaag
    44101 ttcaagcgtc caatacatat gctaatagtt tttcaaatta actcagaagg gaacccaaga
    44161 gttcctgaaa aaacgcacaa atgtatcaaa gaaatatttt ataatgcaat taaattctct
    44221 aatttgccag gatgggcatc ggagctgaaa accaggactt taattttcta acgcaggcca
    44281 ttcaggaagc cctggcagga gtccggcgga aagagggcgg acctttcggg gcggtcatag
    44341 tgaaggacgg gttgatcatt gctgccggga acaaccaggt attgcttacc atggatccaa
    44401 cggcgcacgc cgaggtggtg gcgatccggc gggcctgcca ggtcctggga aattaccagt
    44461 tgcatgactg catcatctac tcctcctgcg agccctgccc gatgtgcctg ggggccattt
    44521 attggtccag gcccaaaaaa atcatttatt cggccacccg cctggatgcc gccggggccg
    44581 gttttgacga ttcgtacatc tatgaccaat ttgccctgga aaaccaggac aggaaaatcg
    44641 agaccctttg tttaccggtc ccggaaagga tggatccgtt caactggtgg gaaaaatggg
    44701 gggataaatc cttttactga gccggctaaa cctgatttgg cggaaacccc ctaccggtgg
    44761 tgaaatggat ccgggggatc agaggataag catggcgtcg ccataactga aaaagcggta
    44821 ttttttcttc agggcctgct ggtaagcttc catgatcagt tccgtccctc cgaaggctgc
    44881 agccatgatg agcaggctgg atttggggag gtgaaaattg gtaagcatgg cgttggcgat
    44941 gctgaaatca tagggagggt agatgaattt attggtccat ccttcctggg gtttcatcag
    45001 gccatgggcg gagacggcgg tctcgatggc ccgcatgacc gtagtcccaa ccgcgcagat
    45061 ttttttgttg ttggccatcg ccttatttac cgcttccact gctgccgggg gcaccctgaa
    45121 atattccgct tccattttgt gcttggataa atcttccacg tcgatgctcc ggaaagtgcc
    45181 gaggccgaca tgcagggtga cttcagcgaa atgggcgcct ttgatttcca ggcgcttcat
    45241 cagttctttg gtgaaatgga gcccggcggt gggggcggcc acggcgccga tttctttggc
    45301 aaaaacggtc tgatatcttt cttcatccag gctttccgca ggccttttga tgtatttggg
    45361 caagggggta tgccccagga ttttcagctg ggcctggaag tcgtcgtcgc ttccatcatg
    45421 gaaaaaccgg atggtccgtc cacggctggt ggtattgtcc accacttcag ccaccaggat
    45481 ttcgtttccc tttttgtcgt taaagtagag tttgtttccc acgcggatct tacgggccgg
    45541 gtcgaccaaa acgtcccaga gcctggcttc cttattcagt tccctcagca ggaagacctc
    45601 gatcttggcg ccggtttttt ctttcttgcc gtacattctg gccgggaaga ccttggtatt
    45661 gttgaaaatg aaggtatccc cgtcttcaaa gatgttgagg atatctttaa aattcttgtg
    45721 ttcgatttta ccggtatccc ggtgtacgac catcaatttg ctgccatccc tttctttgga
    45781 aggatattgg gcaatggcat ctttgggtaa aacgaagttg aactgggaaa gtttggtgcg
    45841 cataagttct ttttttacgg aaaaagaggt gcaaatttac aaaaattcca gcggagaaac
    45901 cggcttattt ttcaaacaaa ttgcaaatac aacctgaccg cacggtcggc ttagacgcgc
    45961 atgcgggggt aaaaagggcg gttcgcggtc aaaacgcggc ggttcatcga aagacggccc
    46021 ggattcccga aaagcggttt aatttagagg gagcaaaaaa tattttccaa ggccatgttt
    46081 tttcgacttt tttatgaagg agtggtgcaa tccttttcgc agctggtagc caataagctc
    46141 aggacttttt tgtctctcct gggcattacc attggcatct tttgcatcat atccgtcctt
    46201 tcggcggtgg attcgctgga agacaatata aaaagcagct tcgaaaaact gggaaacgac
    46261 gtcctctaca tcactaaaat gccctggaac gaagaccccg gccagaatta ttttaaatac
    46321 ctgcgcaggc caaacgccac ctaccaggaa ctcgaggcgg tgaaaaccaa ggtcaggaac
    46381 gcatccatgg tcgccctgca ggtctttatc gggggcagca acctgaaata ccaaaacaac
    46441 agcgtcggcg gggcctttgt gctggccgtg agttacgatt acaacgacct gtataagata
    46501 gaatatcagg gtggcaggtt cctcaccccc tttgagtatg aaaacgggtc ggacaaggtg
    46561 attatcggcg ctgaaatcgc ggaaaacctc tttaataccc tcgacccggt cggcaagtcc
    46621 attaagctga aagaccggaa aatggaagtg gtcggtgtca ttaaaaaatc cggcaaggac
    46681 ctgatcaacc cggctgattt tgaccgggcg gtcatcgtcg ggtataatac cgctaaaaag
    46741 ctggtgaatg tcaaaaacaa ccgcctgtgg ggcaccaacc tcatggtaaa agccgctgaa
    46801 gggtcagatc tggaagacct caaagccgaa gtcatttccg tactaaaagc cgagaggcgg
    46861 caaaaacctt atgaagaacc caacttttcg atcaatgaaa tgtccatcct caccaatatc
    46921 ctggataatt ttttcggagt actcaacctg gtgggcattg tcatcggatt ttttgccatc
    46981 ctcgtgggca tgttcagcgt ggcaaacatc atgtttgtca gtgtcaagga acggaccaac
    47041 atcattggca tcaagaaggc gctcggcgcg agacagtact ttatcctcct tgaattttta
    47101 atcgaagccg tcatcttgtg cgtcgtcgga ggcatattgg gcctgagcct ggtctttctg
    47161 atccttaaag tgctttcctc cgtcttcgga tatgtcatgt tcctttccct caagaatatc
    47221 ctgattggag tcagcctctc catatttatc ggtatcctgg ccgggtttat accggcattg
    47281 aatgctgcaa aaatggaccc tgtggtggcg ataaggaagt agtcagtaat cagtaatcag
    47341 tagtcagtag tcagtagtca atagtcaata gtcaatagtc agcggtcagt agtcagtagt
    47401 caatagtcaa tagtcagcat tcagcattca agctcactgc tcgcaaacga ctctcgtttc
    47461 tcaataagtg gttcggtcga ctcgacgtgg ggatggtcgt gcagatcata aaagtgatat
    47521 tccagctaag ctgatagcag atagctgaaa attgaaagct gaaagctgat ccctggcctt
    47581 atttcgcttt agcggcgacg gctttgaatg cctcaggatg gttcatggcc aggtcagcga
    47641 gaaccttccg gttgagcagg atatttttgg aagagaggcc atggatcaat ttggaataag
    47701 aaaggccttc cagacgggca gcggcgttga ttcgcgcgat ccaaagggcc ctgaaagccc
    47761 ttttcttggt tttgcggtcc cggtaagcgt atttccaggc cctttccagg gtatgcttgg
    47821 ctgcggtgag aatgtttcta cgagctcccc aaaatccttt ggtggctttg agtaattttt
    47881 tccttcttgc tctggaggca acgtggttga ctgaacgtgg catttgaaga tgggttagcg
    47941 gctttccagg gactttttta aggtactttg ctcctgatgg gccgggttaa aaaatggaag
    48001 tcggttaacg caaggcgagc attcgcaata ctcttttttc atcggcctgg ctgaccaggg
    48061 ccatatcgcg gttggccagt ttggcttttt tggatttctg gcgaagccgg tgggacgtat
    48121 tcgcctggaa tcttttgact ttacctgttc cagtgacgcg aaaacgcttc ttcgcactgg
    48181 aatttgtttt catctttggc atatcgctta attaaaaagt ggggcaaaga taatcttttc
    48241 ctgtggatcc tgtcaaaaat gtttgccccg gaaatccggc tattttttga ccgttccttt
    48301 gcggggggca agcaggatga acattttttt cccttccagt ttggggaggg cctccgtagc
    48361 ggcgatgtct tccagttcct ttatgaattt gagcaataaa agttctccgc gatccttaaa
    48421 aaccacgctc cggcctttaa aaaatacata ggcccttact ttggaccctt cctgaaggaa
    48481 attgcgggca tgcctaacct taaattcaaa atcgtggtcg tcggtattgg ggccaaaccg
    48541 gatttccttg acctcggcct tgatggcctt ggctttcagt tccttttctt ttttttcctt
    48601 ctgaaagagg tatttattga catcaatgat cctgcagacg ggcgggtcgg cattcggtga
    48661 gatttccacc aggtcaagat taagcagctc agcccagcgc agggcctgcc tggtcggata
    48721 tacgcccggc tcaatgggtg gttcgttgtc tatgaccagt tcgtgaagat tttccccgac
    48781 gagccggacc tcggggaccc tgatttcccc attgatccgg aatccataat cttttttaat
    48841 cggtgccccc cggtagggca ttcgtggctt aaaagtgggc ttttgctgct gcaagtttat
    48901 ttatgtttgg ttaaaaaata tcaagctgga ataaaggcag gctcctgcaa accgctgacg
    48961 gaatattgat tgcatgaaaa atctgcaccg taatacaagg gatcagcgga caaaactaaa
    49021 taatattttt cattcaggag gctggcctag gaagtcgctt ccttgtgaag catgattttt
    49081 aatccggtct tttcctcatt cagggttgct tccaggatag acccttcttc cggtccttca
    49141 ttcaggatga attcggccag cggatcttct atgtatttct gtatcgcacg gtttaaaggc
    49201 cttgccccga actgaggatc gaagcccttt tcagccacaa actttttagc ttcaggggtc
    49261 agtttcaact cgtagttgag gttgcccagc cttttggcca gtccgttgag ggagaggtct
    49321 atgatctgga agatctggtc ttcagaaagg ctattgaaaa tgatgacgtc gtcaatccgg
    49381 ttgaggaatt caggcgaaaa ggtctttttc aaggcgtttt ggatcaccga cttcgtattg
    49441 tcctcagccg cttccacgcg ggccttggta gcaaatccaa cgccctgccc gaaatctttc
    49501 aactggcgga ccccgatatt ggaagtcatg atgatcaggg tatttttgaa atcgatttta
    49561 cggccgaggc catcggtgag taaaccatcg tccagcacct ggagcaggat gttataaata
    49621 tccgggtgtg ccttttctat ttcatccaac aggatcaccg aataaggttt tcgcctgact
    49681 ttttcggtga gttggccccc ttcttcgtac ccgacataac ccggaggcgc cccgatcagc
    49741 cggctcacgg aaaacttctc catgtactcg ctcatatcaa tccggatcag cgaatcttca
    49801 ttgtcgaaaa gatagcgggc aagggctttg gcaagttcgg tttttccgac tccggtgggg
    49861 ccgaggaaaa tgaaagtgcc gatcggtttt ttagggtctt tgagcccaac ccggttacgc
    49921 tggatggctt tggtgatttt ctctatggct tcatcctgcc cgatgatcac ttttttcatg
    49981 tcatcggtca tattgaccag ccgtttgctt tcgctttggg cgacccgttt gaccggaata
    50041 cccgtcatca tagcgatgat ttcggcaata tcgtccgtat ccaccgggta ttttttgacc
    50101 ttggcttcct cttcccattg ttctttggca gattccagtt tggcctgcaa tttagattcc
    50161 gtatcccggt attcggcggc tttttcgtat tgctgatttt ttaccgcgct gtttttgagt
    50221 tccttgatcg cctctatttt ttgctcgatc tcttcgatgt gttttggaac atgaatgttt
    50281 ttcaggtgca ccctggcgcc cacctcatcc aggacgtcaa tggctttatc cggtaaaaaa
    50341 cggtcggtga tgtaccggtc gctcagtttt acacaggctt tgatggcctc atcggaataa
    50401 ttgacgttgt gataatcttc gtacttggat ttgatgttgt tgaggatggc tatggcttcg
    50461 tcggcagatg gcggatccac catcactttc tgaaatctcc ggtcgagcgc cccgtctttt
    50521 tcaatgtact ggcggtattc atccagggtg gaggctccaa tgcactgcaa ctcacccctg
    50581 gccagggccg gcttaaaaat attggaggca tccagcgatc cggtagctcc cccggctccc
    50641 acaatggtat gaatttcgtc gatgaaaaga atgacatccc tggatttctc cagttcgttc
    50701 atgatggctt tcatgcgctc ttcaaactgg ccgcggtatt tggtgccggc caccagggcc
    50761 gccaggtcga gcatgacaat ccttttgttg taaagggtcc ggctcacttt tttctgtata
    50821 atccggaggg caaggccctc cacgatggcg gtcttgccga ccccgggttc tccaatgagg
    50881 atcgggttat ttttcttgcg gcggctcagg atctgggaaa ccctctcgat ctcattctcc
    50941 cggcctacga tggggtccag tttgtcttct tcggcaagac gggtgatgtc ccggccgaaa
    51001 ttatccagta cgggagtgcg ggatttgttg ccgcctttcc gtggaaattt ggaagagccc
    51061 tcttcctcaa aattggattc ggattcactt tgggcctggg caaaatagtc gctgttggcc
    51121 aggtcctgct cctggatcac atactgcaac tccgaacgga aggtttcata gtccacgtcc
    51181 agttcaccca ggatccgggt ggcttcgctg tctttatgtt ttaaaatgga aagcatgagg
    51241 tgctccggag ccacagtgtc ctgtttcatt tcttttgctt cgaggtggct caggcggtaa
    51301 atgcgttccg catgtttgct caggggtaaa tcctcccgca cggcagcaga aggaggatta
    51361 ttggccagct ctttctgcag cactttttca agcttatatt tcaactcggt acagtcgact
    51421 tcgaggcttt ccaggacttt aacaaccaga ttgtccttgt ccttgagtaa accaaggagg
    51481 aaatgctcag taccgacaaa atcatggcgc agccggttgg cttcttcccg gcttttgcga
    51541 atcacatcct gaaattgcgg tgatctttta ttgctcattg gtaaaaatta atggtcaaac
    51601 aagtttacgg tttttaaaac agatccacaa atcattttaa tcatgtacct gttcttgaga
    51661 aattaacaaa ttatccggca ttagtttcat gttttataaa aaattaagta aacactaact
    51721 acttccggtt ttgcatttct tttaaccgat tttaaatcct caaatttcga cccaaatgcc
    51781 ttttatggac aaaaaaatct aattttgcgt ctcaaaatga cgaaatgaca tgaaatacgc
    51841 aatcgataag gaagacagat atgccatttt aaagttaaat gaggagaatt tgaactcgtt
    51901 gatcgccccg gaccttaaat cggaatttat atttctgagg aacgaagggg tgcgaaacct
    51961 gatcctggac ctgtcccagg tcaaatatgt ggattcgtca ggtttgtctt ctatattgac
    52021 tgccaatagg ttgtggaagg attttggttc tttcatcgtc accggcgtcc aacacgcccc
    52081 cgtgcagaaa ctcattgaaa tttcccggct tgaaaccatt ctcaacctcg tgcccacggt
    52141 ccaggaatcc attgaatttg ccatgatgga agatcttcag aaagagctgg aagacggcag
    52201 cgacgagtga ccggtaagcc tctcacaccc catggcccgc tttgaagtac tcatcctggg
    52261 gagcagttcc gccctcccgg cctatgggcg gcacccttcg gctcagctgg tcaatctcca
    52321 cgaaaacctt ttcctgatcg attgcgggga aggcactcaa agccgtctcg ccgaatttgg
    52381 atgcaagatg caccgcatcc gggctgtttt tatttcgcat cttcacgggg accattactt
    52441 tggactgccc ggattgctta cttccctgaa cctcctgggc aggaccgggc ccctggatgt
    52501 gttctgccct gaaggccttg aacccctgat ggatgaaatc ctccgcctgg gacaaggcga
    52561 gttgcaatac aaaatccaat ggcataccct ggttgcaggc gggaagaaaa aaatttttga
    52621 agaccgggtg atggaagtat tttcatttcc gctggcccac cggatcacca cctttggatt
    52681 tatgttttgt gagaaacccg gtatgcgtaa attaagaccg gaaaagatcg caggcagggc
    52741 tatttcctac gaaacgctta aaaagctcaa ggcgggtatc gatgtcgtgg aagagtccgg
    52801 cccgacttat ctgttcagcg aatttacaga agatcttcca tcgcccagga agtatgccta
    52861 ttgttcggat accctgcccc tgcacggcct ggtttccatc atccgcgaag cggacctcct
    52921 ttaccacgag agcacttata tggaagaaca cctggaaaaa gcaggtctga attatcacag
    52981 caccgcctca caggccggtg cgatggctgc ctcagccaaa gtcaaaaaac tgatcgtcgg
    53041 tcatttttca tccaggtata agcttttgga tgagctcctg gccgaagcaa gagccgtttt
    53101 cccaaattcg gaattggccc tggaaggcaa aaaatttgaa gtaggcggct aaggaaggaa
    53161 aaagtgactc cgctgggact cgaacccagg gcccatacat taaaagtgta ttgctctacc
    53221 gactgagcta cggagtcaac ccttttgtat aaagcgctgc aaagatatct ttttttgata
    53281 aaataccaag caatcgtcca gatcaaaaat gggctcgacg atgcatcaga agggcaggtc
    53341 gtcttcaaat tcgggtgggg cggaggtagc cggaggaaag gaagacggag cctgtggtcc
    53401 actggctttg gcagacggcg atgccgggct gccgggggaa tcggattcgg tcacccgcca
    53461 ggctttcaca tcggtgtacc accggccatt gtattcccgg ctttcgatat caaaatcgat
    53521 actgagatcg gtccccggga tcagtttaag tcctcccaat ttatcgcccc aaaccgaaac
    53581 acagatcttt ctcgggtatt ggccttccgt ttcgacgatg atctcctgtt ttctccattc
    53641 cccgtttttg ccttgtccgg tctgtgccgg aagcacttgt accagttttg ccgtgatttg
    53701 cataagcctt taaatatata agttaatcaa ttgttcatag agttcctgct tgccgctgag
    53761 ttgggcgggt tcgccgtttt gaaaagccag gtccctcagg ttttcgaggg tcattttgcc
    53821 ttcttcaaac aatttgccct gtccctggtc gaaggatgtg taacgggcag tcagccactg
    53881 gctgtagggc gactcccgga gcatctgatc ggctatgatc agcgacctgg caaaaaggtc
    53941 cattccgtgg atatgagcga taaacaggtc ttccaggtcg gtgctgtttc ggcggatttt
    54001 ggcgtcaaaa tttatacccc ctccctggag gccgccggcc ctgaggatga ccagcatggc
    54061 ttccaccgtt tcgtaaaggt tgagggtgaa ctggtcggta tcccaaccgt tttgttgatc
    54121 gccccggttg gcatcgatgc ttcccagcat gccggcatcg gccgccacct gcagttcatg
    54181 ctggaaggta tgccccgaga gggtggcatg gttgacttcg acgttgattt taaaatcctt
    54241 ttccagccca tgggccctta gaaagccgat gacggtttcg caatcgtaat catactggtg
    54301 tttggtgggc tccatgggtt tgggttcaat aaaaaaatgg cctttaaatc cctgtttccg
    54361 ggcatagtcc ctggccattt gcaggaacct agccaggtgt tccttttctc tttgcatgtc
    54421 tgtattcaac agggagaggt aaccttcccg acccccccag aaaacataat tttctcctcc
    54481 aagcgttatg gtcgcatcca gagcgttctt aacctgagtg gctgcataag tgagaacgcg
    54541 gaaatccgga ttggtagccg ccccattcat atacctggga tgggaaaaca ggttggcggt
    54601 gccccaaagc aggcggatac cggattcttc ctgtttggtt ttggcgtaac cggtgatcgc
    54661 ctgcaacctt ttttccgact ccgccagggt gtccgcttcc tcgaccaggt caaaatcatg
    54721 aaaacaataa tagggcgccc cgactttgct gataaattca aaggcggcat ccattttatt
    54781 ttttgcccgc tccatgggat cacggccttc gagccagggc atgggacgcg tgaccgatcc
    54841 gaaaggatcc gcgttgccat tgcagaagct gtgccaataa gcaacggcaa atttcagatg
    54901 gtctttcatc tttttcccgt ttaccacacg ctccggttcg taccatttga aggccagtgg
    54961 attctttgac tggcgccctt cgaactggat ttttccaatc cccttgaaat attcccggtt
    55021 cccgatggtg atttttctag tcatgttatt tttagttttt cttttcttga ataacgaggt
    55081 tgatgtttgg ggtgccgaga ttttctcccg ggtggtcggg gtcggccaat tcgatttcaa
    55141 tatggttgtt ctgccgggct tcaagacggt tagccccggc ctccttttca aagcggatct
    55201 gatctacttt aaaactgtaa aacgtattga agcttccccc gatattaaaa tgtttgacaa
    55261 cgagggtatt gctgccgtta tccagaggga gttcgataaa tcggacatgg tcgggcgggt
    55321 gcgcgtatcc ggtggaatag agctgtttac cgttgagcca gatcataaag gcgtcatcgg
    55381 tacccatggc gcaaatcttt ttttccggct gggaagcgag gatgttgaac aggtagtagc
    55441 aggcagacat cggcggcatg gtcacctgaa ggaattttcc ttcttccgat cccggtatgg
    55501 gattccaggg agtcaggtca ctgccccatc cttcagtggg ggacatataa gagatcagtg
    55561 cgggatgact gttcatatcc agtccgggat atggtccttt caggttggca atgctcggta
    55621 ccgcattcgt tgattcaggg gcctgacgaa aggtcttgta tttgctcaag tccaggattt
    55681 cctcatgccc gtttaccttc agtctgatcc gcttgttttt ttcttcctgg gtgtagaaaa
    55741 gcatcatgga atagtctatg tctttaggat tggagatatc ccagctatac cgtgtaactg
    55801 aaggcgccgc ggaataataa ttggtaccgt ccatccccca ggagatgttc gcgttgcggg
    55861 cattcagttc atattttcca gacgcgtcgg aataaaccag ctttgcaggg gtgatatcca
    55921 actggtctcc catatactga agccttaaaa tggttgaagg ttcaaaacgg agcgggatca
    55981 tgcccaggtc aatggtagtc actttgcctg tcaccgaata gcttgcaatt tctgcaggat
    56041 tgaccaggga ccagatccca gaaataggat tgttaagccc gtagagaatc aggctccctt
    56101 catcgggaat ccgggtaata tgtataaata cgtttccggg cgaataggtg aaaactcccc
    56161 agggcttatc actgatcggg aagggcctcg tattgtaaat cgcttcgcta tgcaggttca
    56221 gccattgacc catcccgaga atgagttctt tttcaaaagg atcgacggag ccatctccct
    56281 ttggcccgat attgataagg aagttacccc ctttgcttac gacctgggta aggtcggaca
    56341 gcatttcctg gagccttttg ttgaggtccg gtcgttcctg ccaggagcgg tagccccagg
    56401 ttgctttatt aatggacacc ggagtttgcc atggttggtc aaacttaact tccgggatct
    56461 ggttgtcacc caggacgata aaatccccct ggccgttgcc caggcgcccg ctgatcaggc
    56521 attccggttg aagatccttg actactttgc ggatttcctt gctctgcgcg ggggtgaggg
    56581 tccccatatc aaaccagatt tcgcggagcg ggccgtaatg ctggcaaagt tcggtcacct
    56641 ggttgacgtt aagcctatgc agggcttcgg aaatggaatc cgcattgtgg ccggataccg
    56701 gcgtggcgcc ctctggtgcg ttccagtcga tcaacgagta ataaacaccg aatcccaggc
    56761 cgatcttctt acaggcctcc gacatttcac ttacgaggtc tcttttgaat gggctcgctt
    56821 tggtaatgct gaattccgag tgccggctgt caaacatgca aaatccatca tggtgttttg
    56881 cggtgatgat tacagacttc agtccgccgc gataggccag attggcgatg gaatccgcat
    56941 tccaggaatc cggattgaat tgagcggcgg atgcgcggta ttcctccgcc gggatgggtg
    57001 catgggccat gatttgttca ctgtagccct tggtcaccgg ctggccctgg tagactcctc
    57061 catacatcga gtacagacca aagtggacaa acaggctcag tttatcattc ttccatttgg
    57121 tgatggtttc gttttcccct tcggtaaagg gtaccagggt aggaataaaa gcttcttttt
    57181 tacaagcagg caggaaggcc aaaaaggtca ggaaaaccgt aaggtaccgg atcatagggt
    57241 ggacaataag gttttttcca attgattttt ccatgtatgg taaagttcgt ttaggacgga
    57301 acccaaggca gggtccggac tgagttcttc tattcgatgc agggtttcga aagcggtgga
    57361 cggagtatac acccccaggc caatgccggc tccgcgtgca gcaccggttg ctccatccgt
    57421 atcgtaaaac tccagaggca cctggagcaa gctgctgaac atttgacgaa agacggggct
    57481 gaggaataaa ttcgccttac cggccttgat cgcccttggg gtcatgccgt tggccgtcat
    57541 gatatccacc ccataacgga gtgcatatac aatgccttcc tggatggccc tggccaggtg
    57601 cccaattcca tggcggttaa aatcgagatt catcaaatgg ctctgaacca tccggttgcc
    57661 taacatccgc tccgccccat tgccaaaagg ataaaacaac aagtggtccg aaccggccgg
    57721 gacctgggcg gcttgctggt taagtttatc gtaggtcatg gtgcccccca ggtttttttt
    57781 caaccaggag ttggcgatgc cggtcccgtt gacacagagc agaataccaa ttctgggatc
    57841 cgtggctgta tggttgacat gagcgaatgc attgatccgg gattgttcat ccgtggccag
    57901 tttctgttgg accccatata cgactccgga agtacctgcg gtggccgcta tttctcccgg
    57961 ccggagtacg ttgagggaaa aggcattatt gggctgatct cctgcccggt aacaaaccgg
    58021 ggtgcccggg tgaagccctg tttcggaggc ggcctgtgag ctcagccggc cctggatcgc
    58081 aaaggtcggt accagggaag ggcaccaact ttctgggatc cggtagtgcc ggaataaagg
    58141 cagggccagg ctgtcctggg caaagtccca aaacattcct tcactgaggg ccgggaccgt
    58201 ggtgttgatt tcccccgtaa gtttaaaatt gataaaatcg ccgggaagca ggaaggcatg
    58261 cacctgttca gcgagggagg gttcattttc aatgacccac ctgagtttgg aggctgtgaa
    58321 attacccggt gaattcaaaa ggtggccgag gcaataggag gttccaagat ccttaaaagc
    58381 ggcatcgccg atcggtacgc tgcgactgtc gcaccagatt atggatggcc ttaggacctg
    58441 ccctttatga tcgattaata ccaggccgtg catctgataa ctgatgccga tggcggatat
    58501 atcccgcccg ggtatgcccg tctggaccag gattttccgg accagggctt ttatatgatc
    58561 ccaccatacc tccggttcct gttcggccca tccggggtgg agtgaccgca tgggcatctc
    58621 ttggtccggc acggatgccg ttgccagggc ccgctgggac gatagttcga tcagcgaggc
    58681 tttgataaaa gaactgccca aatcaatacc gaggatatac ataaaatcag ggctcaaaaa
    58741 taacaacata atgccgatcc gctccataaa taccctgcct gatcccaccg tttcaatcga
    58801 ataggcttaa tttcaccatt gattgaacca tggatagtca ggatgaacgt ttggatgtcc
    58861 gggtggtgtt tgcttttgct gccgtgtaca tcatctgggg atccacttat ctgggtattt
    58921 attttgccat cctgacctgg cctcccatga tccttggggc cgcccgattt atcatcatgg
    58981 gagtactgat gtattcctat atccattttg taaagaagga gaggctggac ctgcccttgt
    59041 tccgttttgg actgatatcc ggatttctga tgttttttat cggcaacggg gcagtcattc
    59101 ttgctgaaaa gcaactgccc tccggtctgg tatccattat cgccgggacc gtcccgtttt
    59161 ggttgttgct gctggataaa aggtccggtg ccgcccggtt caggaacatg cttacctggc
    59221 tgggcctgat ccttggtttt gccggggtca tcctgctttt taccggcagg ctggagtcgg
    59281 tacgcatgga ttcgggaatg atctggagtt acctcatcct gatcgtgggc accctgggct
    59341 ggaccaccgg gacgctttat tcgaaatacc atgaagtgaa aggatcggtg cttgccaagg
    59401 tcagcgtgca ggtcctgtgc gcaggtttcc tctttttact ggccgcctgg atcaaagggg
    59461 aattcagggg tttcagcctg tctcaggtga gcgatacttc ctggatcgcc ctttggtacc
    59521 tgatcttttt cggatccatg atcggttatt tctgttatat gtggttattg aacagggttt
    59581 caccccatgc ggtgggcact tacgcctatg taaatccggt ggtagccgtc atcctgggaa
    59641 atgtactggc aggggaaatt ttatcaagcc gggaattcat cgccctgttc ttgatcaccg
    59701 ggggattatt gattttgtac ctgagcaaga agccttgggc tcccagattc agatgatttc
    59761 aatttcgctg acgaacagga acccgttgac cggatcgtcg tcggcgggca gccgattact
    59821 gaccacgatt tttacaaaac gcagcctgcg gtcaatccgc ggaacggccc aggtcaccag
    59881 gccgtcttcc gggtgcgctt caaagttttc cactttggtt ttgatcaatt ggaaattttc
    59941 accgtccccg gagccgtaaa tgcttacctg gagtggtgga aaaattcctt tttttaccag
    60001 ggagagggaa tgaataagaa tggagttgac cggtcttgac tgcccgaggt caaatatggt
    60061 ctcgaggtcc tggttttcat aacccagcca tttttttgat ttaaaatcga ggtccccttt
    60121 gaggccgtcg caaagcacca gggcgccttc actcatatac tggggcgaag gggtggtcaa
    60181 cagccgggct ttgtattttg cttttgaata aacttcatca cgaataaaac cggacaggcc
    60241 gtcggggttg atggctatgg ccaggatagg ggagctgtgg ttcagtttta ccggaccggt
    60301 ataaaggttt gaagccactg agggcaggct gtagttttgg gaataataga tattggcttc
    60361 gggatcattg gtggacatgg tgaccaccag gctgtcttcg aagggtttgc ccggcggggt
    60421 cagggtgact tccggcatgt tttgataaaa accgatttca gaaatattga cagtgttgcg
    60481 gaaatccgtg aaaatgattc tcgctttttg ggtcctcacc cagggaaaac gcaggaggcg
    60541 ggtatagcct accgtggtcc cgctggcttt ttttatccac tggccatcct ccaggatttc
    60601 aagttgaaac ccggccaccc gctggcctaa ctcaatctgt tcacagatct tcaggatatt
    60661 gaaccagacc ggtttttgcc attcaaagga aataaaaggc gcggcattgc cttcatccgg
    60721 gctccagtac gtatcccggt ggccgtcgtt ggtatttttg ggcaggtgac tgatggtgaa
    60781 atcattatca tccgcgcggg cggcccgggc caggtcattg gaaaagatct gcctgaggct
    60841 gttgctgaac actttcagga tggtggcatc tttttcagga atgagaccct gtctgtccgg
    60901 ggggatgttc aataaaagga cgctgttttt ccctaccgaa ttaaaataga tgtccagcag
    60961 tttttcgggt gatttcacga gtcggtcctc cgaagcatga tagaaccatc ccggccggat
    61021 ggaaacatcc acttcggaag gataccattt catcgcaccg gcaacaccca attgttccag
    61081 gttggctatt tcttcacggg tttcatcgag gacgggcttt accatattgt cgtggagatc
    61141 gtccgcggtg accgggatgg aatcctgaat gggaatcacg ctccactccg tctcccggcc
    61201 gtagccggat tcggtgccca cccaccggac gtcggggccc ataatggcaa tgacggcctg
    61261 aggttgaagt tccctgacca gcctaaaata tccctgccag tcgtacacct gttttttacc
    61321 gtttggccct tccccgacgg cgccgtcaaa ccagacttca tccacggggc catagttggt
    61381 cagcaattca cgcagttgtc ttttgtaata atcgttgtag gcttcagatc cgtaagtcgg
    61441 ctcatgcctg tcccagggag agaggtataa acccaacttc atattctgtc tgacgcaggc
    61501 atcggccagt tccttgacca catcaccctg tccgtttttc caaaggctgt tttttacaga
    61561 atggctggtg aatgcgcttg gccagaggca aaaaccgtca tggtgttttg cggtgaggat
    61621 cacgagacgg aaccccgctt ctttcaaaac ggagacccat tgatttgcat ccaggttggt
    61681 gggaaggaaa atcttcgggt cttcctggcc gtccccccac tcgttgtccg taaacgtatt
    61741 caccccgaaa tgaaggaaag cggtcatctc cagcttttgc cattccagtt gacggggggt
    61801 gggaattacg tgcgcggcct taaataagat atccccatgg tcgtctgtgg aatcaatcag
    61861 gattaagttg gcaaaccggg tctcctcatt cccaaaacgg caggccgaaa ggacgagcag
    61921 gaaaaacagg aataaaattg aatttttgag caggccgtac atgagtctgt ctcaaaagta
    61981 ggaaattaaa caggttcaga cagtccggac ccccttatct gagttctgca tttatcgttt
    62041 ggagcacgga atttccggga cagagttcgt aatattccag ggcattgagt ggcgtgtcca
    62101 cggtattcag gatatcgtgc agatttttgc gttccgcctc gtaatacaac aagccggtta
    62161 ccacttcctg gttcatccgc gaacggtgga ttttggtaag ggaagaaatc cggtccgtgg
    62221 ggtcccagtc cagggccagt ttattgatct ggagataggt gccgtcatgc atggtgatgc
    62281 gcccggactc cccgctggca atgtccgagg tgatttcttc ggcaaggggg acaaaatcaa
    62341 gctcggcaac ggtctcgaga tggtcccgga cataatcata ggatttggtc gagcctttgt
    62401 tgttgttgaa ggtgacacag ggggaaatga catcgataaa ggcaaaaccc ggaaaagaga
    62461 cggcggcttt gatcaggggg atcaattgct ccttatcccc ggagaagctc cgggcgacaa
    62521 aactggcacc caattcgagg gccattccgg ccatgtcgat ggtttcaaac aaattggtat
    62581 gccctgattt gttgatgctt ccgggatcgg ccgtagccga atcctggccc ttggtgagcc
    62641 cgtaacaacc gttgttcatg acgatgtaca ccatattgag attgcgccgg atggcatgca
    62701 caaactggcc catgccgatg gaagcggagt ccccatcccc cgaaacaccc aggtagatga
    62761 ggtctttgtt ggccaggtaa gcgccggtgg ccacggaagg catccggcca tgcacggaat
    62821 taaagccatg gctgttgccc aggaaataag tcggtgtttt ggaactgcat cctataccgg
    62881 agagttttgc aacccggtgc ggcggaatgg acaattcaaa tatgccttgc acgatggccg
    62941 cgctgatgga atcatgtcca cagccggcac aaagggtgga caaggcccct tcgtattctt
    63001 tccgggtata tccgagtttg tttttggcag cctcaggatg ccgaaaagct ggttttacgt
    63061 aagtcatgtt gatcggttaa ggtggttgaa gcatacccga tgtgttccat gattttgtca
    63121 tggatcagca ttccggtgat ggggagacca tcgtaattaa gtaccgggat cagccgggca
    63181 gggtctacat ccagttcatt gataaggagg gttctcattt gggcatcgcg gttttgctcg
    63241 actacaaata ttttctggtg gtcctggata aaatcggcaa tggaaggatg gaaagggaag
    63301 gcccggatcc ggcccagatc gagatggatt cccttctctt cgagcaggtc ggccgcttcc
    63361 agggatgaac tctccgaggt cccgaaataa agcatgccca ttttattttc ctgttcctga
    63421 ttctgccatt caggagccgg caggtaattt ttaatagtgt cccatttctt cagaagccgg
    63481 tccatattcc gcacataagg ctctgattct tcggtgtacc gggccatttc gtccctggaa
    63541 gtaccgcggg taaaaaaact gcccttggtg ggatgggttc ccgggatggt ccggtaggga
    63601 attccgtctc catccacatc caggtaccgg ccgaattctt tgatctcatc gagttgggcg
    63661 gcattcagca ccttgcctct tttataattt ctgcggtcgt cccaaaccag gggctgactg
    63721 ctgtgttcgt tcatgccgag gtcgagatcg ctcatgagga tgatcggagt ctgcaactgt
    63781 tcggccaagt caaaggcctc tgcggtcatt tcaaaacatt ccctgggggt gctcgggaag
    63841 agcagggggt gcttggtgtc cccatgtgag gcataggcac aggcgaggat atcggattgc
    63901 tgggttcggg tgggcatgcc ggtcgatggg ccaccccgct ggacgtcgat cagtacgagg
    63961 gggatctctg caaaataggc cagtcccagg aattcactca tcaaggaaat acccggcccg
    64021 ctggtggccg taaatccgcg ggctccgttc caatttgccc cgatggctat ccctatggcc
    64081 gccaattcat cttcagcctg tacgaaggca aattttttct tgccggtttt agggtccagg
    64141 cggtaggagg aagcatattc gtcaaaggcc gacatcaggg aggtcgacgg ggtgatggga
    64201 taccaggccg cgacggtagc tcctccatag agggcgccca gggcacaggc actgttgcca
    64261 tcgatcatga tcaggtcttt gtttaggtcg cgtcgttcaa tcctgaattt ggacggaagt
    64321 tcaaaatgat caatcaggta ttgccggccc aggtgcaggg cctttacatt gggggtgatc
    64381 agtttttcct tacccttaaa ctggtccgac accatttgtt caaggaccgc catttccata
    64441 tccagcaggg tagccacggc tcccacatag accatgtttt taaacaggag gcgctgtctc
    64501 gggtcggcgt aattctgggc gcagatttcg gtcatcggga tcccgatatt gttcacttcg
    64561 tcccggagca aacccttcgg catgggtttg gtattgtcat agatgaaata ccctccggcc
    64621 gaaacgctgg caatgtcctt ggccaggctc tgcggattga tgctgaccat gatatctatt
    64681 ccgtttctcc gtccgagatg gcctttttca ttgacccgga tctcatacca ggtgggcagg
    64741 ccctggatgt tggaggggaa gatgttttta ggtgaaacag gcacccccat ccggaaaata
    64801 gcttttgcaa ataggttgtt tgcactggcc gatccggtgc cgttgacatt ggcaaaccgg
    64861 atgacgcagt cgtttatttt ttctatacgt gacatggagt ccctgcttta gcggtgttgt
    64921 acaagaatcg ctgcatatcc caggcagcag tcgggcatcg ttcggcgcat aaaccgcaat
    64981 ggaggcaaac gttttcatct ttgaccatta cccttttggt ggccaggggg gaggaaacgt
    65041 aaagatcctg gctcaatagc tgcgcgggtg cgttgaggcg gccccggaga tcctcttcct
    65101 cgccgttgtg ggtaaaggtg atgcaggagg tagggcagat gtcggcacag gcgtcgcatt
    65161 caatgcagcg ttcagcagta aagaccgtct ggacgtcgca attgaggcat cgctgggctt
    65221 ccttgaaagc aagcaggtca tcaaacccca gttcgacttc cttcttcctg ttggtaaggg
    65281 tttccgattt actggcgtga ggcactttgt acctgaggtc ggtgactacg gtgctgtcat
    65341 agctccattc atgaataccc attttctggg taaccaggtt ggtcagggga tccggccggt
    65401 tgtaaaggtc ctctccattg cagaaaagat cgatggacac ggccgcctgg tggccctggg
    65461 ccaccgccgt gatcacattc ttaggaccga aggccgcgtc gccgccgaaa aatacccggg
    65521 gaagggtgga gccaaaagtg attttgtcca ccaggggcat tccccattta tcgaattcaa
    65581 gcccaagatc cttttcgatc cagggaaaag cattttcctg gcctatggca atgatgacgt
    65641 catcggcctc aaaaaatata tccggttcgc cggtgggtac cagggagcgt ttcccttggt
    65701 catcatattg ggcagccacc ttctcaaaca gcattcccct gagcttgccg ttttcatgga
    65761 caaaggcttt tgggttatgg agattgaaaa aaggaatgtc ttcgtgttgg gcgtcttcaa
    65821 tttcccaggg tgaggctttc atctcatcaa acggactccg caccaccact tttacgtcgg
    65881 tgccgcccag gcggcgtgaa gtcctgcaac aatccatggc cgtgttgccc ccgcccagga
    65941 ccaggacttt tttgccgatc ttatccacat ggttaaacgc cacattggcc agccattcaa
    66001 tgccaatgtg aatattggcc tgaatttcct ttcgtccggg gagcccggac aggtctttgc
    66061 cccgcggggc cccggtaccg acaaataccg catcgtaacc ggactccagc aggtctttga
    66121 gactgtgtac ataggttttg aaatgcgtgg atatgcctcc aatgcccagg acatatccca
    66181 cctcttcatt caacacctcc tccgggaggc ggaaggccgg gatttgagac cgcatcatgc
    66241 ctccgcccag gggctgttca tcatagaggt cgatttcata tccgaggggg gccaggtcac
    66301 gggctaccgt cagggaagcg gggcctgcgc ccacaagagc aattcgttta ccattgccgg
    66361 tttccggtgc cgagggcatc ctggagacca cttcaccttt aaaatccgca gcgacgcgtt
    66421 tgagacggca tatggccacg ggctcttttt ccacccggcc gcggcggcag gcaggctcac
    66481 agggccggtc acaggtcctt ccgaggatcc ccgggaagac attggattcc cagttgacca
    66541 tataggcgtc gctgtatcgt tcagcggcta taagtcggat atattcaggt accggggtat
    66601 gggcaggaca ggcgtattga cagtcgacta ctttatgaaa gtactctggg tctttaatat
    66661 ttgtcggttc caaacaattt atcttaaaaa ttttgggacg aaaattacgg tttttaagcg
    66721 atttactgtg gtgaaaaaaa attgccgccg gggttccgac gacaatcttt ctgttcactc
    66781 ctgccggcat ttcctgccgg caagtcaagg cctggtaaac aacaggagag accgtaatca
    66841 tgtggtctct cccgaagtcg gtccaccgcg ggggtgttta aaggttgggt tgaggggtat
    66901 agcggagata gggtttgatt ttccggaacc ctttgggaaa tctcttttcc gcatcggcat
    66961 cggatatgga tggaaggatg atcacatcct ccccgttttt ccagttgacg ggggtggcca
    67021 ccccgctgtt agcggttagt tgcagggagt cgatgacccg gaggatttca tcaaagtttc
    67081 tgccggtgga tggcggatag gtgatggata atttcagttt cttatccggt ccgatgataa
    67141 atacgctgcg caccgtggtc ttttcagcgg ccccgggatg gatcatatca tacagctcag
    67201 ccacgtggcg gttttcatcg gcgatgaggg gataattgac cttggtcacc tgggtctctt
    67261 caatgtcgtg gatccaggtg gtatgggaag ccagcgggtc gacgctcagg gcaaggattt
    67321 ttacattgcg attgtcaaat tcgggcttca atttggacat gtatcccagt tcggtagtgc
    67381 agacgggggt gaagtctgcg ggatgggaaa acaggaccgc ccagctgttg ccgagccagt
    67441 caaaaaagtc aatttctccc tgggaggttt gagctttaaa attcgggacg aggtccccaa
    67501 gtctaagtgc catagttttt tgaaattaaa taattaagga attaaagaat taataaaatg
    67561 aataattaag gggttgtcat ggtcattgtt ggaatattag taggtgggca tggactggtc
    67621 aatttctttg gcccaggcca ggatgccccc ttccagatta tacaggttgt caaatccgta
    67681 tttttcctgc agcatttcaa tgacatcggc gcttcttttg ccgctgcggc aatgcacgat
    67741 cacctgcttg tctttcgaaa ttttctgcac gtggctggtg acttcgcttt tgggcatgag
    67801 ttcgccgtgg atatttgcga tttcatattc atacgcttca cggacatcca cgatctggat
    67861 attggcattc cggtcgatca actcctggag ttgtttgaca ttgatggact tgacgggcag
    67921 tttttcggcc tggtcggtag gtttttcaaa gccgcagaac tgctgatagt caatgagacc
    67981 ggtctggctg ggattttcgc cattgagggg attgtccttg cgccttttta ttttgagggt
    68041 acgggtttca aacgttgcgg catcaaacaa aaacaacctt ccgctaagag gttccccaac
    68101 cccggacagg actttgatca cctcactggc ctggatgctt cccaggattc cgggcaggac
    68161 gcccaatact ccgccctccg cacaactggg tacgagtccc gggggtggcg gggtcggaaa
    68221 gaggtccctg taattggggc ccagctcccc ggctttattt ttataattaa aaacggtggc
    68281 ctgcccttcg aaccggtaga tggaggcata gacattgacc tttcccagga gtacgcaggc
    68341 atcattgacg agataacggg ttggaaaatt gtcggttccg tccgccacga catcgtaatc
    68401 ccgtatgatg tccagggcat tgtcgctggt cagcgcggtc tggtggggaa tgattttgat
    68461 aaaggggttt agggcttcca gtcttttttt cgcggcgatc actttgggga tcccgagatc
    68521 atccctcgta aagagcacct gtctctgcag gttgctgtca tcgactacat caaaatcaac
    68581 caggccaatg gtcccaacgc ccgcagcagc aaggtacagg aggaggggag ccccgaggcc
    68641 accggtgccc acgaccagga ctttggcctg tttcagtttt ttctggcctt cgatattaaa
    68701 ttccggaagg atgagatgcc ggctgtaccg ggccagttct tccggtgaaa attttatttc
    68761 tccgttcacg gtcatttatt attgattttt aaaaggttcg caatcagagg ccgccggcga
    68821 tggccggaat gatgctgatg gtatcgcccg ggttgacccg ggtctcctgt ccttccaggt
    68881 cctcaatatc gttttccccg ataaaaatcc ggacaaagcc cctcagtttc tgatcttcat
    68941 taaagaggtg tttgtccagg tcgggaaatt gttgggtgag gtcgcggatg gattccagga
    69001 cggtgccttc ctggacggct accgcggcct ggtttgaagt gaattttcgc aagggggtag
    69061 ggatgatgat ttttgccata tgagtttaat tatttgaatg gtagttgatt tttagagttt
    69121 cttcctggaa tacccgttgt tcggaaagcc tccaggaccg ggtatcgagg gcctgtccct
    69181 ggtggacgct ggtgatgaaa taagacaact ccggaagggc gtcctcgagg tcgcgtgcgg
    69241 aaggccttgg gggatggtcc ggatgggaat ggaagacccc caccagcagt ttgttgtttt
    69301 ccagggcata acgttcagcg cgcataaatt ccaggggagg gatggtgtaa tgcactttgt
    69361 ggttttcatt gctgttgttg actacctcac ggacctcctc tgcacgcaac ggttgaccgg
    69421 cttcgccaaa tataaatccg cagcattcat aggggtaggt cttttccgcg atatcgtgga
    69481 tggccttcag taccgatggt ttgatctcaa tgtccatgat catttataga cttcctggta
    69541 tttggatgcg tcgtcggcaa acagggtgac aatggttcct tcttccatgg tctttgcgac
    69601 ctgcagggcc ccggtcagat tggctgcggc ggaagggctg atgagcaggc cttctttttt
    69661 ggccacctca tcgatcagtt taaaggcatc ataggatgaa acctgcagga tctggtctgg
    69721 cgtgccggga tcgtagattc cggggacttc cgcggtttcc aggtgtttcc agccttccag
    69781 gccgtgaagc ggcccatcgg gttgaagggc gatacaccgg atggccgggt tataggtctt
    69841 tagccttttg ctggtcccgg taaaggagcc ggtggtcccg aggccagcca caaaatgggt
    69901 gatatagccg gaagactggc gccagatttc aggggctgtg ctgtggtaat gggccttcca
    69961 gttgttttca ttgttgtatt gatcacaata aaaatagggt tgatccattt tggcatagat
    70021 tgcaagggca agctgcctgg attcgtcggt ccctccgtag ggagaactga ggactagttc
    70081 cgcgcccagt tttttcagaa tttgctgtcg catcctggag gcgttttccg gcatgacgag
    70141 tttgattttc aggcccaggc tggcggcgag ggtggccagt gcaatcccgg tattgccgct
    70201 ggttgcatcc aggatggtat gatggggctg aagctccccg gtatgaatgg cttgcctgag
    70261 catattatac gcggggcggc tttttacgct gcccccaaac tgctgccatt ccagtttggc
    70321 gaaaattttc accttttcgg tggaaaaatg atgaaggggg aaaagcgggg tgccgcccac
    70381 ccatcttccc agggattcca ccgatttcac caggtccgta ttgctccagg taatggtctc
    70441 gttcatatcc gttttataaa tcagaaagcc ttccgggttt tatctcgaaa ggctttctga
    70501 atgattctga aattatttat tgcatcatta gatgaccttt cgaaaacaac cctttacaac
    70561 aacaatacgc acaggtacaa gccggtccgg ctgcctcatt tgtggtattt gttttgtaat
    70621 aaatggtcat tttcaaatct ggtctaaaat caaaaagcct tcgggttttt ctgaccagaa
    70681 ggctttttga tattttatta tatccgttaa cttctatgtc agatgaggat gcctacagca
    70741 acaacaacat gcacaacata taccgataac agatttcatt gtttcttgtt cacaaaagta
    70801 caattgccgt gccagttttg caaatcatcg tttctttcat tccgttaatt gagtgttaag
    70861 aatccggcat ttccataccc ctggcatttg cccggaaaaa gagaaaggag cataatacta
    70921 ttttagcaaa gaatagtata aataaaaact tagaacgtgc ggaagtggtt gtcgtaatcg
    70981 gccttgccgg gaaaaaaggt tcagagggat tccggatcgc gggtccggat taccattcca
    71041 tctggtcact tttcgacttg tcctcgtcgt ggatcgagtc gtcgtggttg tcgtgatttt
    71101 catcgcggtg ggcaaactgg tcgtagtcaa aatccggaag gaggttggtt tttacatgat
    71161 tgaccacatc ctggaggcct tctacaaagc ggttgaaatc ttctttgtac aggaaaatct
    71221 ggtggcggat ataaccattg ccgtcagacc gccgggtgct ttccgtaagt gtgaggtaat
    71281 aatcattgcc ccttgtcttc attacatcga aaaaataagt gcgtttacgc ccggctttca
    71341 ccttgtggga gtacacgctt aaattacgtt gggtaccatc gttttccacg gtaaggttaa
    71401 gtttttgatt ggtaccaaat atacaatttt gaaaccaatc aaaaaacctc gacttcaccg
    71461 ggtgcgttgg actcttccca tttttgccgc tcgaggagtt tataataaag tccttttttc
    71521 cgcagcagtt cgtcgtgggt gccggattct tccaggcgcc ctttatggag gacaaggatc
    71581 tggttgaagc ccatcaggtg gttgaggcgg tgggtgatca ggatggcggt tttgtgattc
    71641 aatacctgtt caaaggcctg gatgatattt ttttcggtcc gggtatccac tgcgctgagg
    71701 caatcgtcaa gcaacaggat atcgggattc atggccaatg ccctggctat ggctaccctt
    71761 tgtttttgcc cgccagacag gctgaccccc ctttctccaa ggatggtctg cattttatcc
    71821 ggcagttgcc tgatctcctc ttctatggcc gcatgccttg ccgtttgcca gaccacctct
    71881 tcgccgagtg aaggtgcgcc caggcggata ttgttgtgga tggtttctga aaacaggaaa
    71941 tgatcctgcg gcacataccc gatttttttt ctcagggcat tcaggtcata gtctttgatg
    72001 ttgaccccat ccacgaggat ttccccggag gtgacgtcaa acagccttaa taaaagttct
    72061 gcaatggtgg ttttgcctgc ggccgtccta ccgatgacgg ccaggcgcga gccgggttcg
    72121 atgacaaagg atacccggtc cagggcaagg gtcccggtat tgggataaat aaaacttacc
    72181 tccttaaatt ctatcctgcc ctgcaacttc tccggcttgt aagtgccggt ggattccacg
    72241 gctggtttaa gggtaaggaa ttcattgatc ctcttcatcg aggcctcagc ctgctgcacg
    72301 atggaagcca gccagccgat ggcggtcacc ggccaggtca gcatgttgat gtagatcaca
    72361 aactcggcga tattcccggc cgtcaccgca ccggattgaa cccggtaaac ccctgcccat
    72421 acgatgatta cggtactcag gccggtaagc agcatgatca gcgggaaaaa caaggcattg
    72481 acccgggaaa ggtcgagggt gtgggtcttg tactcttcgg tttcctccct gaaaaacccg
    72541 gacatggctt tttcctgcgt ataggatttg acgatccgga tacccgaaaa gacctcctgc
    72601 gcaatgctgg taagtttggc cagctgacgt tggatcaggg tgcttttacg gttgatcagg
    72661 gtgcttacat aataaatgga tatggaaagg aaggggaggg gcaggagggt gagcagggat
    72721 aaagtaaggt ccaccttgac catactaaaa accaccagga cgatcagggc cgcgagatca
    72781 atcgcataca atacggcggg acccaggtac atcctgacct ttgaaacatc ttccgtaatc
    72841 ctggacatca ggtcccccgt ggtctggcgg cggtagaagg actggtccag ggtcgaatag
    72901 tgggtaaaaa tatttttgcg gaggtcatat tcaatcagcc tggagattac gatgatggtc
    72961 tgtcgcatga aatacatgaa tatccccatc aatactgcgc aaccgaatac gatcaggcca
    73021 aaacgaacca gttggggagc caacctttga cgggattccg tatcgggatt ttttaattct
    73081 tcgaccacca ggtccagggc gtcccggatc atctggggct ggacaatccg gaaataattc
    73141 atcaacagga caaaaacaat gcccgccagg aaacgccatt tatattttag aaaataagta
    73201 ttgagggtcc agagctgttt caacgaagcg acaattagga ttgcagacaa aaataataga
    73261 aatcaacggg tgggtgattt tttatcccgg tttaatcccc ctttcgaaga cggatggatt
    73321 tcttacgggg tgctgaagtc cagatagggc aataactttt tccaaaaggt cgaatcaaaa
    73381 gcctgaatcc ggattactgc agccggatcc ttaaacgggc cgtgctggcg gatatagttt
    73441 gccaatatgc ggctctcttt tacgctaagg tagggatgag cttttttaag gggcaggtcc
    73501 aggtgaaccc ggatgagtcg ggtgatcggc gaagggatca gatagggctc gatcgcatgg
    73561 taagtggaat cgggtaagcc atagacttct ttcagttgcc cgcgctcatg gaaaccgcct
    73621 aatgcctccc tgaagtttat tatcctcctg gccagcacgg gccctattcc gggcaggcgt
    73681 tcccaggcag aagtgtccga tcggttcagg tcgacggttt ccggggaggg ggggatctcg
    73741 tccgctatcg ggttttcaga atgaggattc gcatccggtt cttcctgttc aaataccagg
    73801 aaaggcagga tgcgttgaag cagtgagtct tgcaggccgt atacgcggct tagatctgcc
    73861 ggttttaaaa accgtccacc ttttttccgg tagttgtcca gggtatatgc cactttgagc
    73921 ggcaagcctg ccgcggtcag atcccggaca ctcgcggtgt tggggttgag gctaaggttg
    73981 aggttgaggc taaggttaag gttaagggct gtgcctttcg ctttgccggc attttcaaat
    74041 accaggtgag gggttaattt atccagcagg gactgatcca tgccgtaaat tttaccgagt
    74101 tcctgcgaag acttgatctt tcctcctttt gccaggtatt tcatccaggt gccggctacc
    74161 cttttgggaa cgccggcttc caggagcagg tccagggtaa cccggttggg atcgatgcgg
    74221 agcgcagttg gcactgaggt cttggattcc acaggggatt ccagggagga ggggccggga
    74281 tttacatctt cccctttctc caaaggaaga atgacatagc cggattgcgg tggaaaaaaa
    74341 tggatataca aatagaccag gccaagaata aataccataa ataagagggc aaactgggat
    74401 ttgcgctgat gaagggtgta cctgaaaaaa gccgggaaag gtttgaattt cattttattc
    74461 ggatgcgcag ataaggagat tcaaccggtc gaggggaagg accatctttt aggtccgcgg
    74521 caaatgaaat taaatcggga aggccaggtt ttttttgggt ggttattgaa ggggttgcgg
    74581 caggcctgtt ttttagcaag ggctggaatt tggagtattc tgacttggtc ttttgatctt
    74641 ccagggcgat ctcgaaatgc ttttctccga agataatttg atttccttcc aggcgcaggg
    74701 ggcctgagag caaggattgt tttccaggat ggaagtaaat gatgttctct tcccggagtt
    74761 tgtatttttc caggtaggag cgtggaattt tcatactgtc ctgaataagg tagagaaaag
    74821 cattttcccg gtgttttatc cacagccagg gccggttttt atgggtcccc gcgaccaatt
    74881 ccgttttggt ttggttcctc ttttcttcga ggatgaggac agacaggaaa aggatgccgc
    74941 acaacaacat gcagttgaag gaaaaccggg ttttttgaaa atgaacgtaa gccatccctc
    75001 cactgagcag gatcagaaaa agggtatctg ccaggcttgg ccaccagccc tggatcttac
    75061 ccagcggcag ggtgtagatc caggagaccg tttcgttcat ccaaagtacc ccataccgga
    75121 atgggatact gatccagggt accagatgcc cggccagggg ccatagggcc agggtaaaca
    75181 atccgcctcc caggatcagg taagagaacg gggtgctgat cagccccgtg agccagaaat
    75241 aaaaggcaaa ctggtggaaa tggaatccga ccaccgggta tatagccagt tgggcggcac
    75301 aggaagcggc gatcaccttc accaagacct ggactatcct gttttcaacg gtgatcaggg
    75361 tacggaaggg ttcatacaac agaaataaac cggccatggc cagaaaagac aactgaaagc
    75421 cgatgttgta aatatcgaaa gggttgatgc ataaaagcac aaaggcggct gccgcaagga
    75481 tattgaccgg tgaagaatcc cggagcagga gcctggacac ggtgagaaat gaaaacatca
    75541 gggcggcccg gagtatagcc ggcgctgccc cggtaagcag ggcataagcc cagatgatcc
    75601 cgatcaataa aagagcctgg agctgcaccg gttttttctt gccgatccgg ataaaaccga
    75661 acaaatactg gaaaatatgg gccatgattc cgacatgaag cccggaaacg gccaggatat
    75721 gcaccgcccc ggtggccgaa taggcattga acaggtcctg gtccattttt ttatatgacc
    75781 ccagcaacat ggccgaagcc accggataat ccctgtccga gggaaccgct tcgcggatat
    75841 acccgtccag ccgggccctt aaggattcca gtaaaccggg acctgggtga tgcacgaccc
    75901 tgaaatcagc ggtatccaga taggtgaaat ggtgaatatt tcgccggtgg aggaaaaggt
    75961 tccagtcaaa ctcaaaggga aatgaaggtt taaaaggctc ctggatgcgg aagcgggcct
    76021 ggatgacatc ccctgaagaa gggttataag aaccgggctt tggtatccgg atcagcaggt
    76081 tgccatgaac gttccggttg attgaatcgc cgatctcaag gaaggtcctg ctgtacccat
    76141 tccaataacg ctctttttcg tcgacctggt gaatttcaac aaccagttcc tggtactgtt
    76201 tttccggatc aaaaaagtga tttggcaagg tcctatcatc cctcagggcc gcccaggcaa
    76261 aacccagggc ccaccaggcc cccagggaag ccaaaccaaa ccatggggcg ggcatcacct
    76321 gccgtttggc ccggcggtga gccccgatca ttgccaaaag ggatgggacc aggactgcca
    76381 ggccaagcag caaggatcct gtcgtgcggt ttgagcaaag gatgcccagg gcgaaaggga
    76441 ttaggattct cacaaaagga actctcgccc agggtagctc atattccgtt cctgaccgcc
    76501 agggcatggg cggtcaggaa ctggaagggc tgtacttaaa gcgcccgaaa aaccagacca
    76561 ctacaaaaca aaggaggggt acgatatacg ctttctggat gctgccggtg ctgtctgaaa
    76621 tcatacccat gaacaagggg aacacggcgc ccccgaaaat agccattacc aggaaggagg
    76681 atgcgatttt cgtttcttcg cccagccctt tgatcccaag tgcaaaaatg gtgggaaaca
    76741 tgatggacat aaaaaacggg acggcggcta tggcatacac cgccactttc cctgaagtga
    76801 cgagggccag ggccaacaag ccgatgttga tcaggctgta aagggagagc agcctggcag
    76861 gggctacata tttcatgagc caggtgccaa caaaacggcc gaccataaat cccaccatgg
    76921 ctacgcttcc ccagatataa gcggctgttt tttcaggcat gtcgatcagg aaacgggtca
    76981 ggcgaatgaa aaaactgcca acacaaacct gggctccgac atagaagagt tgggcgatca
    77041 cggcccacag cacatgccgg tgccggaata ctttcagtga aaaggaatgg cccgtatccg
    77101 agctgtcttc ctccttgatg tccggaatat gggagaacag aaaaaacaag ccaaccgtaa
    77161 aaacgacggc agccatgacc aggtatggga tcttcacgct ggatgcttca aaagacaggt
    77221 actgattcag ttgatctacg gggagggcag ccagttcctc tcccgttttt tcaataccgg
    77281 agaggataaa ttgcccgccg atgatgggtg cgagaaaagc gcccactccg ttgaaggatt
    77341 gggcaaaatt gagccgttgt tcagaggtgg cgggatcacc caatttggtg atataagggt
    77401 tggccacggt ttcaagaaaa gtggccccgc tcgcaatgat gaacaaagcg gtcagaaaca
    77461 tcgtataact ccttgccccg gcggccggat aaaacaggag cgccccaacc gtatacaaac
    77521 ccagtccaag caggatcccg tttttataac cgtatttatg cataaaccac ccggcaggca
    77581 aggccatcag aaaataaccc aggtacaccg ctgagtcaat cagggcagac tgggtatcgg
    77641 tcaactggca ggctttcttg aggtggggaa tgaggatggg attcaggttg tggagaaaag
    77701 cccataaaaa gaaaaggctg gtcaccagga taaaaggcaa gaggtaattt tttttcaagg
    77761 attgcataag acaggttagg ctaggatgat tggaataggc cgggagggtt aaaacgcatc
    77821 atgaaatatt gatctgtcct tcatagacct gtacggcttc gccacaaagc cagatctggt
    77881 cataacgtcc gggacccagg cgctcaaaac ggacagtaag ggggcctccc ctcgctt
//
LOCUS       DAOQXT010000005        76119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_335_length_76119_cov_7.570769, whole genome shotgun sequence.
ACCESSION   DAOQXT010000005 DAOQXT010000000
VERSION     DAOQXT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 76119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 76119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76119
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_335_length_76119_cov_7.570769"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3770)
                     /locus_tag="PKM27_01175"
     CDS             complement(<1..3770)
                     /locus_tag="PKM27_01175"
                     /inference="COORDINATES: protein motif:HMM:NF039454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6443 domain-containing protein"
                     /protein_id="HNR05891.1"
                     /translation="MKVNYFSKSSTINQASSSEKSNQFDENNFLQSVASKEIETQPDI
                     FYFNAGGQSAKFYFTASKTIVMSEVKDLAITPYWNSTTDDIDSFLVKDASGINYWFTA
                     TEQTTVYYPSDDNWPRYHTNFVYKSSWYLSKVISPNATETVEIKYISEGSAYDPNQFV
                     HPGTNWSRTFSHGYQSGGCPDPTCPCGDNSTDNAASVNTFTIAGRKYLDEVIFKKGTV
                     AIEKLKFASSSNTYTGTGGGRKLDKISLYRGVNAGTELVHQQFYYSDQTGRLTLDSIK
                     ERNAVASLSKQAHAFYYNATDLPSFTSNSIDHWGFYNGASNGTWPTNKLLPVINCDGV
                     NPNDVSCPGMDNPTSGANREPNETYAQAGTLIKIVFPTRGYTEFTWEGNRAITSDFIC
                     NTNNTERLVGGLRIKNIKTYAAPGTLLTNKQYNYIQSNGNCSGILFYHPDYYTSSTFT
                     TYTWSIAQLCSQQAINRYCVSYMYFASSRVPLGSIQGSLVGYSRVEEVFAGKTVYHYK
                     NYAPGTNSWNLVNEFDIQDLGQLKLIEEYDDAGKKLHSTGYKYSFDISETRRSQSFYG
                     MIVEAKQLQDNKLKLCLNGGVYTWVIDGDNNNCSQSAVYQSKWERKHYLITQDWIYQK
                     EVFDTVFYYTGAALAGSVKKTITHTYGNTNISSPTESVVTNSDTKVYKRVTKFANDVS
                     QSDMIGKNMIGIPLENENYVDNVRVYRTKITFNQYDPSTGLPGTGAWVLPYRFYENFS
                     TGGSDVLRETIDRYHPAGTNVQEAHKEYDQVNTLIYSHNETLLSARVQNALKSECAFT
                     GFCTTEASQGGWTISNTGSLQADADGKTGSGYWTGTGNISKSSHPAGKYFLSYWIKDQ
                     ATQAGISGATTNTYVISSADANGWRFVQHTLTLNSASTLTLTVDDPFVAVDELRLYPV
                     DALMTTYSYNRNTLLASGMANENSIPGKFEYDELLRMTGVKNYNSYYLKTHEYTYKTG
                     TGTYNSVKTRDVLQDAVTSTGTVNGLSGSNVRRQFNYFDDIGRALQNIGVGQSPTSKD
                     VILHHDYDSYGREPNGYLPFTYTNGTPGNYLSTASSELVTFYNNQFGGSEGTYAKTVT
                     EYENSPLSRVNKVTHPGSVFNTKPVVYVYSCNTGSNEVRNFRTASAWYAVNSLNKVKV
                     INENGDSTVTYTDLLGLKVMEARNTVKTYYLYDDRQNLVQVIQPLAAASGHSDATKVD
                     TSTSVKNGSFIYTYDASNRLSIKKIPSGGTTTYTYDRLDREVVRSDPNGFRI"
     gene            complement(4168..5361)
                     /locus_tag="PKM27_01180"
     CDS             complement(4168..5361)
                     /locus_tag="PKM27_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR05892.1"
                     /translation="MKDAFFILAIFCQFISFPISGQTTWTKTSLQEQYPVSNILSLGK
                     DLMVSTMGGGIYKSINNGDNWSEIKSGLSKSVIFSLINNSKTLYAAAYGDGIYKSVNE
                     GESWEFISSTPDPYIYSLAVSGKNLIAGTWSGIYYSQDGLEWIKAEIKGDKKHSIAFT
                     FRTHKNKIYAGSGRYIFVSEDHGISWRSQEAFTVFDIQAFFENKDTLLAGSSGDGILA
                     SVDGELWQKKLPNKSETELKNVTSLVSDSTGLLVASANTNILNQGDKMVYGKSDLRAK
                     VVHYHKGKYYAGTYGGGLWRLDTNKKISELEVRNSLELFTALYPNPAFKNSVLEYSFN
                     QAVQIKIDIIHPEGKIYRQLNLGMQGAGQYQLKISDLNLGSGIYYLRLQADQELKVLR
                     LVIIN"
     gene            complement(5806..6408)
                     /locus_tag="PKM27_01185"
     CDS             complement(5806..6408)
                     /locus_tag="PKM27_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNR05893.1"
                     /translation="MEIYSLVPEDVRGLIFDLDGTLADTMPSHVKAWSLTGRHFGVDI
                     TAEMINEMAGAPSYQVIKTFNKRYGWDIDPLAGKTIKSDYYLSLLDELEEIQPIAEVY
                     ELVKHYHGRLPMAIGTGSSKVNADRVIRALGLDHLIPTVVSADDVLHPKPHPETFLTC
                     ARLIGIDPADCLVFEDGPLGIQAAVDAGMRVMVLPEFKLL"
     gene            complement(6505..7422)
                     /locus_tag="PKM27_01190"
     CDS             complement(6505..7422)
                     /locus_tag="PKM27_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNR05894.1"
                     /translation="MNQRRSFIKTSLALSAGTLVAPSDVLGLIKEKRKYGVQLYTANS
                     VINDDLKGVLKGLADIGYVHLESAGSPLGGYYGLPAPEFAKLAKEYGLKWVAHHASGF
                     PFRRPGGAPPSANNPNTPPRPPSRSLATHMQELVDEAAQGGLKYLVCSSTPVKTMEEI
                     NQSLEVLQKTGEACKKAGIQFAFHNHTAEFELVEGKRPFDMMATQISADLLKFELDLA
                     WATKAGVNIPELFKTHPGRFPLWHVKDLDKDQKPVEVGQGYIDFEPIFKKAKLAGTQF
                     FLVEQDAAPKPLENLKTSFTNLVKAGSKA"
     gene            complement(8302..9342)
                     /locus_tag="PKM27_01195"
     CDS             complement(8302..9342)
                     /locus_tag="PKM27_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05895.1"
                     /translation="MPWNATGTEMGSIGEPLAFADSGLFDEESILQLTITGAVNKLIQ
                     DRSDAAKAHPVTLVYEGPDGPQSLSLKMKTRGNFRRTQGNCTYPPLSMDFTGQPVDKG
                     LFAGKKKLKLVMPCRGEDYVVREYLVYKLHNLFGPKSYKVRMVHIQFREEGNSKKDFS
                     VTGYLLEEDEAMAARNEATLITKRMMRPENTAPRDFVRMALFEYMIGNTDWSVQYLHN
                     VRLLAIPGEKPVPVPYDFDHSGLVDAPYAKPSEALGLRSVTERRYRGYCMADDSLIRE
                     VLTEFTSRENEIMATVDRQPWQEKNTPETIKKYLGGFFEIIRNEKKWTEAVRYPCDKN
                     GTGNVVVKGLKE"
     gene            complement(9402..10664)
                     /locus_tag="PKM27_01200"
     CDS             complement(9402..10664)
                     /locus_tag="PKM27_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR05896.1"
                     /translation="MDHQELIPHLFRTEFRKITAVLCNAFGMDHIEAAEDIAADTFLA
                     ALQTWPYRGIPDHPSAWLYATAKNKAFNYIQRNKLYSTKILPAQIRNADHRFLPDIDL
                     SDQNIRDSQLQMLFALCKPSLPVEAQIALSLRILCGLGIDEIARAFLTSKETIHKRLY
                     RAKEKLCAEKEALRFPPEHEINERLGSVLATLYLLFSEGYYSEIHDSVMREELCLEAM
                     RLVSLLTEDRHTDRPAVHALLALMCFHASRFKARKGASGEMVLYEDQDETLWDQALIS
                     RGVECLHKASRGEVVSKYHLEAAIAYWHTVKKESREKWEHILNLYDRLLQLEASPIAA
                     LNRVFALSKIKGKEAAIKEAEKLKLLENPYYHTLLGKLYTGLDDGIAEKHFLEALSRV
                     RTRADQNILQRHLSGLKPGFRPGLDFSP"
     gene            complement(10693..11046)
                     /locus_tag="PKM27_01205"
     CDS             complement(10693..11046)
                     /locus_tag="PKM27_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNR05897.1"
                     /translation="MDEFMLIFRHQDGQKLASPEQMEIWMKQTMDWISGITAKGKFVG
                     GNGLLFEDARVVGYNNMVTNGPFGDIKETIGGYIMVRAESAEEAVDFAKGSPVLQGEG
                     NTVEVRKIARRDGIH"
     gene            complement(11731..12546)
                     /locus_tag="PKM27_01210"
     CDS             complement(11731..12546)
                     /locus_tag="PKM27_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNR05898.1"
                     /translation="MDFERLLKYDPLWLALLATLFTWLVTASGSAMVFFFKTAHRKLL
                     NSMLGFSAGVMIAASFWSLLKPAIEIAESRGQVSWIPAIIGFLSGGAFLLVMDKTLPH
                     VHWSRTMDKAEGVKTSWQRSVLLIAAITLHNIPEGLAVGVAFGALYSYTSPEALTGAL
                     TLAIGIGLQNFPEGAAVSIPLRREGFSRRKAFFYGQMSGMVEPVAGVLGAWLVSQVAP
                     VLPYALAFAAGAMIFVVIEQIIPESQTGEETDYSTIGAMLGFAAMMFLDVALG"
     gene            12644..13462
                     /locus_tag="PKM27_01215"
     CDS             12644..13462
                     /locus_tag="PKM27_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNR05899.1"
                     /translation="MKRRDFFKTASPMVLLLANGQITEAGRLGLESMDRRKPILRFAV
                     ASDGHYGQPDTPYQEYFTTLVNRINEEHARRKFAFAVINGDIIHDDKSFCPAAKAALD
                     RLHLKYYVSQGNHDMLTPEEWQANWQMPINLDFKIKKSSFLIASTSNAQGTYLCPDLA
                     WMKDRLEAHRRQDRVFVFIHINPAKLTRHAVDCPQLYELFDRYPNVKAVFNGHDHDEE
                     GIKMKGKIPFVFDAHFGGSWGTEYRGFRVVEVLRDGTIATYILNPVDRIQEGVL"
     gene            13551..14249
                     /locus_tag="PKM27_01220"
     CDS             13551..14249
                     /locus_tag="PKM27_01220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR05900.1"
                     /translation="MVNYLVVGASSGIGQHLAHDLAEAGHRVYGTWFSHPVVTFGPFI
                     QYHPLNVLDDTVSLDFLPESLNGLVYCPGSIKLRPFEKLKTADFISDYQLQTIGAVKI
                     IQAALPKLKQSGNASVVLFSTLAVQLGLNFHSLVASSKGAVEGLVRSLAAEYAPKIRF
                     NAIAPSLTDTPLASPFLDTEHKREINAQKHPLKRIGNTADMANMATFLLSEKATWITG
                     QVFHVDGGLSTIRT"
     gene            14253..15671
                     /locus_tag="PKM27_01225"
     CDS             14253..15671
                     /locus_tag="PKM27_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR05901.1"
                     /translation="MKILLTGVTGYIAHRLLPVLLQQGHQVICCVRDKNRFVADGSQN
                     LGVVEVDFLDKKTLNRIPEDVDAAFYLIHSMSAGSGDFESMEIACARNFRERLEETGV
                     KQVVYLSGIVNETELSKHLASRKKVEDILSGARFALTTLRAGIIVGSGSASFEIIRDL
                     VEKLPVMVAPRWLNTRCQPIAIRNVLEFLSGVIGKPQTYNKSYDIGGPDILSYKEMLL
                     RFAKIRGLKRRIWVIPVLSPRISSYWLYFVTATSFALAKNLVDSMKVEVICKENDLAS
                     LLGIQWIDYDTSIRLALDKIEQNQVLSSWKDAQTSELLNKGISHLIEVPVHGCFIDRR
                     KIKINDVRHTLDKIWSIGGRRGWYFGDWLWQIRGIVDQLVGGVGMRRGRKSETGISAG
                     DALDFWRVLYADKEEKRLLLYAEMKLPGEAWLEFKIDRENILRLTATFRPLGVWGRLY
                     WYAVLPFHGYIFKGMIRKLAAV"
     gene            15780..18269
                     /locus_tag="PKM27_01230"
     CDS             15780..18269
                     /locus_tag="PKM27_01230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNR05902.1"
                     /translation="MKKLARLKTGLSSEQVVASRSAHGSNRLQLQEDRVFWHVLRSVV
                     LEPMFVLLAAACSVYFILAEYTEGVIMLVAILFVSGISFFQEYRSRRAIRALKELSAG
                     QAKVLRNGVEIKVDLEEIVVGDVVLLQEGEIVPADARILASNDFSLNESILTGEAFAV
                     SKNAYDQNQVFRGTLVTSGSAWIEVTEVGLNTQFGKIGLSLKEISVIQTPLQVQIRHF
                     VRSMVWIGSAAFVIVVVFNYLQSGDITHAFLQGLTLAMSILPEEIPVAFSTFQALGAF
                     RLLKQKIVVKQPQYVETLGSATVIAVDKTGTLTQNKMEFGYLYDAPSGLSIRCDEKSP
                     LPLRLIEYAMWASETQPFDPMEITIHDWYGRKAPVDLRSQHSQVHEYPIGGIPPIMTH
                     VFQNHKGETIIAAKGAPEGILRQSRMTMNELLKAEQQALDYASMGYRVLGVGKGSWEG
                     KVWPVSQESFKFEFLGLIAFQDPPKPSISQTLKAFEDAGIQVKMITGDYAETALAIAS
                     QVHFRHNGKILSGNEIMDLREEELKEKVKDVNLYARMFPEAKLKVIEALKAGGEVVAM
                     TGDGVNDGPALKAAHIGIAMGKRGSEVAKNAASLILTDDDLAHMTDAVALGRRIFSNL
                     EKAIRYIVSIHIPIILIVTLPLILSWAFTDLFSPVHVIFLELLMGPTCSIIYENEPME
                     PGTMQRPPVRTGRVFLSFRHLALSIFQGLAITAGCLGIGYYYMQAGHDETTVRTVIFI
                     TLLFSNIFLTLVNRSFYYSVFRTLLYKNSLVPLILFITLLFILAFLYMPFMQQWFRLH
                     TLSLNSVGQCIAVALVSVLWIELWKWGKRKG"
     gene            complement(18304..18867)
                     /locus_tag="PKM27_01235"
     CDS             complement(18304..18867)
                     /locus_tag="PKM27_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05903.1"
                     /translation="MDFGFSYVVWECELIEGNTFWDFRLTLILKEDSIVYGELAKREK
                     YRFWAVQGYFPTHHERIQELMVQYNSFYDTNYRFDEFNKVLAQDYYYSMGCGETGNDL
                     PLQAKQMLRWVETGNIRKLVEWIKSPNFELQAYAVEGVTRIQKKGIQLENKVLEIIDH
                     LKGRNSIILHCAGCIQGLRTPLNNLIF"
     gene            19105..20205
                     /locus_tag="PKM27_01240"
     CDS             19105..20205
                     /locus_tag="PKM27_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR05904.1"
                     /translation="MKKILPASLFLTLVMNAWSQPSPPLRVAIDGMVHDHVNWILGRK
                     PLGDIEIVGIAEPNQQLVDAYVKRYGFDKKLVYPSLEKLVEAVRPEAVLAFNSVYDHL
                     KTVEFCAPRGIPVMVEKPLAANLEQAKTMLTLAKKHHIPLLTNYETTWYGSNEMTYDL
                     VHTQNKIGEIRKLVFHTGHQGPVEIGCKPEFLAWLTDPVLNGGGALMDFGCYGANLST
                     WLFKGKTPESVTAVALQIKPNIYPKVEDEATIILKYDQAQVIIQASWNWPFGRKDMEV
                     YGQTGYIFCQDNRHLSLREGEKKETLPFTAAELPSSRKDPFIYFKNVIRGDIKLSPSD
                     LSAPENNETVMKILEAAKRSAKSGMTIRWSEL"
     gene            complement(20373..21041)
                     /locus_tag="PKM27_01245"
     CDS             complement(20373..21041)
                     /locus_tag="PKM27_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05905.1"
                     /translation="MKHRFSLTFIVVHFTFLLSAQNYLFPVKTSGYHLAPSYSHLNNG
                     DGRSNLWALTGGYTFRGRFTGSLTIGREITKYTVQNNNVKLNTYLGALGFNYLVLKQE
                     QHHTILSFSIDAGYQFNRLIYGSPGDNASIHSFLYGLSVYRNFHFANLFSISPSFGLG
                     RGHSHYRHDPSPLGIREESYSFPYYNYQLAFKLGNFYLRPTIQVTNGNSYLNLMAGWM
                     VTLL"
     gene            complement(21200..21730)
                     /locus_tag="PKM27_01250"
     CDS             complement(21200..21730)
                     /locus_tag="PKM27_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR05906.1"
                     /translation="MKYLNLVIFVIVFNQTQAQKPDKYAACCGAEPIEYLHSDMSIYV
                     PNVFTPNKDGINDLFYPFISGKVVEIIDFTVFTDVGDTVLFYRPTVVYSNLENYGWDG
                     MRKDGLPYIGAFKYSLIMVNRQGDTRVLTGRACRIECGADADEIKQKSGCYFPEQAGP
                     DGKVDRTKGNKEKKCE"
     gene            complement(21756..22169)
                     /locus_tag="PKM27_01255"
     CDS             complement(21756..22169)
                     /locus_tag="PKM27_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR05907.1"
                     /translation="MKHILIFAIIGLHLRASSQCITFAYDAAGDRTARNICPQAIRSF
                     IEPIANNNYQVTHSQLADHYINIFPNPTNDLIEVRSDYLSSNSQIFITDILGRKFYSG
                     TLKDGKIDISSLVAGRYYLRISDGNIVRIVSVIKQ"
     gene            complement(22325..22909)
                     /locus_tag="PKM27_01260"
     CDS             complement(22325..22909)
                     /locus_tag="PKM27_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05908.1"
                     /translation="MDTTNKIKVMDFNELISEKLGLIFQKHHLIIIEQSINHMKLKSD
                     KVVILFNYNEKEKSQNLYVGKKDDLLYAVDEYVLKNAFNSDLKINNVTPDTFAKNLCT
                     FFNGRGKPLIAGNKYALSALEKYVYKEGEIYTMQLLVSQKLDAATKAWEERNYKDFVN
                     FIDQIDKRKLAPSYKIRYKMAINKLNSSKKNKNI"
     gene            complement(22888..28866)
                     /locus_tag="PKM27_01265"
     CDS             complement(22888..28866)
                     /locus_tag="PKM27_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNR05909.1"
                     /translation="MKRKTHILGLLFSIFYLIETSGQGALYTSGTFTKTVNTALPVGT
                     VAASADVDLSGAATYTIPIVVPPGTNGVVPSLSILYNSLGGYSVLGQGWSITGMSSIS
                     RIPKTIYHDGNVAPVELTTGDRYSLDGSRLVLKTGTYGAAGSTYGTESENFATITAFG
                     SIGTGPQYLTVFLKDGRTMEYGGTSNSRAMNTAQTQVMSWLLSKITYPDGNYIEYSYN
                     NGVGVSITFNEINYTGNTAAGLTPYNKIKFNYVSRSDVNTVYEAGTARTQDKFLDNIT
                     ITGESSAAFKIYQFNYGWDNINSYLNEIIEKGSNGSQLNSTIFKYGDQPTIVQRYGTN
                     TTGIQDGNYYSGDFDGDGFSDLVEANKILSDNIEYCSQIRLFKAIPAINQATSFSAPI
                     VKNLAGNFSALKKKSIPDLYTFLTNDFSGDGIDDFAAAKTTGYGGTWAFQFARIYNGS
                     NTTPFSDSVTINAYTGYSKINSTTGHFLFAGDFNGDGKGDLLSILGYTSSPTTIGIHI
                     YYGGSSPSFLTVGTTGTINIPVNNWQSAKRIQVLDFNGDGKSDLMVTSGNNSEIYTID
                     GYVARSIYYAGFPTQDHLPFFGDFNGDRKMDILYRSSTTLNNAPWYKCLSTGTSFDQS
                     AFTFTTVPTVTSEYSGDKILLMDCNGDGKTDIFHGILGTTSSTYHVYFSRGSSFYFYL
                     YTGTTTTLNTMPLTPFDYNGDGKSEILVRTNYLSYFDIYHFMPFGKELFLEKVKNGYD
                     YTTQWIYKRMNESGTFYNKGPTLSGNVNNIQLPMHFVSEIKMDNGIGGMNSMTYNYAE
                     AKLHKEGKGFLGFKKINSDNAASGFRKKSENEFNLTYYVAAPYKDSIILISNGSLVKE
                     VTNSNQFLSQGTGRYWYRINNVLENKVFEGRTLSTANSVFDSYGNVTSQVVNNNSVET
                     TTTTTTYGQYGTPVPALPTSVTVSNTRTGQTAYSSVINMTYNSLGQMLTKVEFVGQPK
                     AVTTTYYYNNLGNQISARISASGITARNIFSSQFDTMGRWPNTTTTPVGVTYTYYHSL
                     WGKPYSVTDIMGLITSFSFDAFGREISMTTPQFYNITTSYNFTTSYGSRYYKQITHPH
                     RPDIKIYNDVLDRERRTETEGWQNQWITTEKTYTANGEVFTTTGPYKSGETGLLTTFN
                     YDGYRRLASIVENKGGTNYTSTLGYVYASGNLTTTSTNPASQVSSKVSDATGKTISAT
                     DYGGTLNYAYYSHGGLKTVINGASTLVTNNYDVYARQSSMVDINAGTTSYDYDALGQL
                     ITQVNAKSQTHTMTYDTMGRILTKTMPEGTTTYEYYQAGSYASIGKIKKITGFAGDLQ
                     EYTYNGYGDLWTDKQTVDGVAHTKTYTYDASYSNVSTVSYPSSFALNYNYDANGYLST
                     VKNTDNSVTIFTNGTMNGYDQYKTYSLGNGKSSTNTYFYGIPTNYSTTGVQNLSLNWN
                     HNSGNLTSRVDGIASKTETFGYDNLNRLTSSLVTGLTTYTTVYSNNGNISSKTDAGTY
                     AYTSSRINAVTQINPSAGTISSASQSLPTYSSFLQPTSLTEGINTLNYTYGYDENRIK
                     SVLIQNGSTTNTRYFFGDYEKDITGGSTKYIHYINAGDGLMAIVIRESGTDTYYYTYT
                     DHLGSILNVTNNIGTVVHNQNFDAWGRYRNYSNWTYSGVTAPPIWLTRGFTGHEHLEL
                     FKLINMNGRLYDPIVGRMLAVDNYVQFPEFSQAFNRYSYVLNNPLKYNDPSGEIIPLV
                     FAGAALIGGGLNLWSNFSKVQNFGQGLSYFMSGFLGGGISVVNPLAGGSYTATTNVLL
                     DISYGHLPQLDNVKDITKYAFHVALDAFGASGAGKMADLLGSSIFNYSWRSFGGYTTG
                     AGTTRIIGETADDIIKYNVPELVIGAEKVIIGADGLIYNAARFATKTGTQQGGLNLFK
                     WGAQQTSKSTGWKIGDYMLHLPNKGLAKLNWKANYSALRSEMNLGKPIFDSYRLPNGN
                     LIPTGGFLNAERYILHTRGWIYSPSSGEWIPPIR"
     gene            29486..30814
                     /locus_tag="PKM27_01270"
     CDS             29486..30814
                     /locus_tag="PKM27_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR05910.1"
                     /translation="MRFFLFFLFGGFTLLSAQNKIIALVGGTLIDGFGGRPIQNSVIL
                     VEGERIKAVGHQGNLAIPPGAEVISTEGMSVLPGLWDMHVHLMINGHSDYAHWDKKYL
                     PLAEEMIMPASAHQLLMAGVTSARDLGGPLEASLKVRDRINSGEIPGPTMYMSGPFLQ
                     HEPYPGTAEFRWGIKGVADARAKVQQLAGAGVDCIKMIDHDQMTMDEVKAIVDEAHKY
                     RLPVVAHSHRPEEIRRGLKAGVDCFEHTGLASAPSYPEDILEMLKERTAQMALGPMFW
                     TPTVEGLYNYEYLRENPEALDNTSWHLGLPDSIIEDIRQSIRYPGRLSYFQLNPIRRP
                     TLQTKINQLKEAGVVLLIGTDSGIPMKFHSQSTWNELEVWVNKFGLDPMYAIRAATYW
                     PSVAMKADADFGTISEGKYADIIAVKGDVLRHIALLQNVNMVMKKGRRVK"
     gene            complement(31038..31991)
                     /locus_tag="PKM27_01275"
     CDS             complement(31038..31991)
                     /locus_tag="PKM27_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNR05911.1"
                     /translation="MKAAVYTQYGPPEVLKIKEVEKPTPKQNEILLRIKATAVNSGDW
                     RLRKADPFAVRFLYGLLRPKINILGSVFSGEVERVGEAVKDFNAGDLVFGHADMSFGA
                     YAEYKCLPENASIALKPANISHLEAAVIPFGGVTALHFIKRAMIKPGQKVIVIGASGA
                     VGSAAVQLAKSFGAEVTGVCSTANISLVKSIGADKVIDYTKEDFTKNGETYDVIFDSV
                     NTLSISRSLRSLSKNGIMILSAAGMSEMIQGLWISKTSNRKVLTGVISHTKSDIVFIK
                     ELIEAGKYKPVLDRTYSLDQIAEAHAYVEKGHKKGNVAIEV"
     gene            32288..32485
                     /locus_tag="PKM27_01280"
     CDS             32288..32485
                     /locus_tag="PKM27_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05912.1"
                     /translation="MIDQVKKIFNRIANLPEKQQIELAKLIEDEISWEQTFASSQEAL
                     AALAKEAIQEYKSGKTSEQDW"
     gene            complement(32900..34051)
                     /locus_tag="PKM27_01285"
     CDS             complement(32900..34051)
                     /locus_tag="PKM27_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR05913.1"
                     /translation="MSLVSKESRWKFYLAIAGILVMALSMIYVRYLAKKLEDGERSSV
                     ELYVKAQEALAKTGSDINKDVTFESQAIEANKTPIIATDENDQVTWGRNYGPLKDEDK
                     AFLAKRLVKLKKQGIQPIKSELNNVYFENSRVLRWLKLFPYIQLMLLLGFLMIGLIGF
                     SASQREQQNRVWVGMAKETAHQLGTPIGAIVAWVENLKLLSSEDPEKMELLDELRSDV
                     TRLELIAERFSKIGSKPTLEKTNLNQQLDQCRNYMEKRASKKVRFDFPALNGHPHFAR
                     INPPLFDWVMENLMRNSLDAMDGTGTIKAEIYEEGDYICIDLSDTGKGLSGNQFKQVF
                     EPGYTTKKRGWGLGLSLARRIIENYHKGKIFVKRSVIGVGTTFTVKLPG"
     gene            complement(34146..34538)
                     /locus_tag="PKM27_01290"
     CDS             complement(34146..34538)
                     /locus_tag="PKM27_01290"
                     /inference="COORDINATES: protein motif:HMM:NF021462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HNR05914.1"
                     /translation="MKATMLFLLTALIYAGLDLLFINGFARAFIRRQVGHLLADKPDW
                     AAGVVFYVLFTGGLLYFAVMPADSLMSAVKNGAFLGVLCYGTYELVNKALISGWPRSM
                     VWVDLAWGLLAGAVTSGLAWWIKGWLNL"
     gene            complement(34535..34957)
                     /locus_tag="PKM27_01295"
     CDS             complement(34535..34957)
                     /locus_tag="PKM27_01295"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNR05915.1"
                     /translation="MKTGPLDRKIYGGTALLCVAALTVSFLLANRTLTPPAQSCYFVS
                     QFGFPCAGCGGSHALQAMIGGNGLTSFFYNPLVAVLLLLILVMGGILLFDLLSNKRIL
                     AFGFKRVMSGLKNRKVSLGLAALLVVHWTYIFFKYFKG"
     gene            complement(34954..35577)
                     /locus_tag="PKM27_01300"
     CDS             complement(34954..35577)
                     /locus_tag="PKM27_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNR05916.1"
                     /translation="MDNSGKRLPATFYQNKDVVHLGRSLIGKVLVSEIDGLRTSGIIV
                     EAEAYRGPDDKACHAYANRRTPRTEVMYKRGGHAYIYICYGMHHLFNVVTGPEEYAHA
                     VLIRALEPLEGLEIMMKRRQLKTNEERITKGPGALSVAMGFDKSFSGHSLVGPESLIY
                     IEDRQIQIDPELIAAGPRIGVESAGACASWPWRFYIRKHRNVSAKRT"
     gene            complement(35567..37036)
                     /locus_tag="PKM27_01305"
     CDS             complement(35567..37036)
                     /locus_tag="PKM27_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNR05917.1"
                     /translation="MYGLKAAILLLSACIGLSGLKPGTKPNLVIILADDLGYGEPGCY
                     GQSRIKTPHLDRLASRGMRFTQYYAGNTVCAPSRCVLLTGKHTGHADIRDNFEMGGFP
                     DDEERGQLPMRSDEYTLARFLKSKAYRTAVIGKWGLGMPGKEGDPQAAGFDFFYGYAC
                     QKQAHNYYPSHLWKNDRWDTLRNGYFSPHQKYPLSGKPLTGDYVAEHYTGPDYAPDLM
                     RREALKFIAEDDRPFFLYYTLPMPHLGIQVPPEELQAYPDFADTPYLGERQYLPHHRP
                     RAAYASMISRLDREVGILMDALAKAGKDQNTLILFLSDNGATPPGTGGADTRFFKSNG
                     LLRGYKMDLYEGGIRVPCIVSWPGKIKPGSVNPSILAAWDWFPTLADLFGLPLKTDEM
                     DGLSFKKALLKNQVMDPDRYLYWEYSSFGGSRAIRKGDWKLIWFSKDNKGQLYNLRND
                     PGEQTDLSDSNRPMRDELYALMQGARSPARIPAWNKYGQ"
     gene            37096..38034
                     /locus_tag="PKM27_01310"
     CDS             37096..38034
                     /locus_tag="PKM27_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR05918.1"
                     /translation="MKFKFIVFTGLCIFSWASAPAQDPMIEQNIPGTSMQFKLILAPA
                     GTAELGPQKIKVNLDAFYIGVYEVTFDEFNCFRERDQDNDQSSNPAIAYKADAVTRPT
                     SQYVNLTFGMGDRDGFPMVSTTQQGALKYCQWLYQKTGRFYRLPTEAEWEYACRAGTS
                     TRFYFGDEESKLADHAWYYENSDDKYHKVGQKQPNPWGLYDMLGNVAEWTSDDYRENY
                     YSLVTADNNNPWMIPSKKHSRTVKGGSYVDDDPDHLSPSYRLKSEAKWQVRDPQIPKS
                     KWWNTDSPYVGFRIVSPLKQPSPSEVEEYFKKAIVD"
     gene            38142..39368
                     /locus_tag="PKM27_01315"
     CDS             38142..39368
                     /locus_tag="PKM27_01315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNR05919.1"
                     /translation="MNSDHPAYLKSDRLSTLSYAIRGPIFDKAHEMQLAGQNIINLNI
                     GNPAPFGLFTPDEIVQDMILNIGEAQGYSHHLGIFPARKAIMHYTQQIGIPEVTIDDI
                     YIGNGVSELIVMTMQAMLNDGDEILIPSPDYPLWTTAVSLSGGRPVHYRCEEQNEWVP
                     DVEDIRSRISPKTKGIVVISPNNPTGAVYPKSVLQQIADLAAEKNLIILSDEIYDKIL
                     YDGNKHIPIASLNPDVFYLTFGGLSKNYRATGYRSGWVILSGAKHRAASYIEGLTLLA
                     SMRLCANVPAQYGIQTALGGYQSIDDLVAPKGRLYRQGKFVHDRVNAIPGLSCALPKG
                     ALYVFPSIDLAQFRFEDDNDFVLKLLTEQKVLLVPGSGFNHYDRAHFRIVFLADTDIL
                     GTALDRMEELLHQYRA"
     gene            complement(39382..42099)
                     /locus_tag="PKM27_01320"
     CDS             complement(39382..42099)
                     /locus_tag="PKM27_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05920.1"
                     /translation="MSKQKSKAENRPSQTANLNILLLNAGRREANEIREAVSQVKNRF
                     KNADLIRADLSIPLDQEFESIPLKPDLETEAAFIQKNVLVIDLGHKNWEEELSRWTEY
                     KGPGSGSRPVALAYCGKRGSCNPGRASLFFPKGYFEKNRDGGNLKGRDLVLMIRNRMQ
                     YWDSANQLFMVNQENPQENQSPVREKWSAFWERAFAGPWSIFRQPGLSTYLKRDVLFQ
                     LLLSGLLAASLLYALLGALNVGNSSDENRYLTQAEKVYHFYRTLGADTSALTRSGVDP
                     QYYNGQIFDNALYTIGKPFGLERNFTFRHLLNALTGWFSILFASLIGLRLGGYRLAFY
                     TALLMTLSPIYMGNVFNNHRDIPLATFTIFGTWSIIRFWDYYPFIHRKYLVYAGLALF
                     LSFASRLAGGVLLTALMGLYSLLFLISQNGLTRLISLDKQTWKPVLGLILTTVLAFVM
                     NLLVWPYGLVDPVNHSLEVFRSSGNHPVALYQIFESKYRLSTSMPDHYVIKYVGITLP
                     VAVLAGVALALFAFLANRIKSGKNILALLFFSFAFPLIYSYLKLGNMYGSWRHFLFIF
                     PFIAICSAVGWQWLFERFPRIRHSLWTAGILVLCLIHPLQYILRNHPFEYLYYNEFIG
                     GPGGAYGKYELDYSLNSLKQGAQWLKKYIRDNHPPGTRLVIATNGSSELAYYFRGFDD
                     SVSVTYTRYYERGTQNWDYAIFPNMYIHPYQLANHIFPKADSLHTIKIDGKPLCFIYK
                     RNSRDDYEAMQAINRNDANLAVQKLNAYLRHNPRSEWAWFQLAYIYAQSNNWPQAQNY
                     LNESTKHHPEFLPSRMLQGIIHFNTGKTTEAKAIFTKLTDEKFDLANTSKWAGMIYEK
                     EGDYRKALEQYGFAMGAGNKQKDLYQKIANCLRQLGDVNQAAKYESMAQ"
     gene            complement(42105..43565)
                     /locus_tag="PKM27_01325"
     CDS             complement(42105..43565)
                     /locus_tag="PKM27_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR05921.1"
                     /translation="MIFIVAYNAERHIAKVLARIPRKDLDKYDYEILIIDDQSRDATF
                     ERALDYQKQNLNLNLTVLYNPENQGYGGNQKLGYHYARIHGFDIVLLLHGDGQYAPEE
                     FDRLVHPIAEGKADAVFGSRMLNKKDALKGKMPYYKFIGNIILTRLQNKILGTHLSEF
                     HSGYRAYAVKALSQLPVERNSNDFHFDTQIIIQLVMAKMQILEVPIPTHYGDEICYVN
                     GTRYAYNIIKASIHSRLQQWGIFYKREYDLEQKDSPYRFKSGYLSAHSRVVEKIPAGT
                     KVLGIGSASGLVADALIQKGCTIKLATLRNQGSQGLLDQEFVEALHKNEYGALLLMDL
                     LETLDQPEQLLDELRDQIRLTDPDIYITVANIGFFITRFQLLFGGFNYGKRGILDLHH
                     KRLFTKKSIKRLCEQSGMKVIGMEGLPAPYPEAFGLNAWSKALVRLNFLLIRLSKGLF
                     AYEYLIQVKAKPVVKHLIRHSIKASELRKQQHLISE"
     gene            43674..45251
                     /locus_tag="PKM27_01330"
     CDS             43674..45251
                     /locus_tag="PKM27_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05922.1"
                     /translation="MKSHPVPPRSSRPFPGFISILALFLLYAVVYGYVFDEKINLNGD
                     NMGYYASAKNIVQGKGFSYAYDIFATRLNEPIGYPLLIAATMLISDSMNLISLVNGVL
                     LFLSILLLLGIAGSLGISRLIQGLTALYILLNPSLMQYAYIHMSEGPFIFFSLLAIFL
                     FLKLKPGIPYTKQPLFYLMAFSLLAMYFTRTAGIILLIGFLLYLLYDKKYLPASVLAF
                     LFLVSLGTWSLYAPASNRYVQQVLMVNPYDAHLGKLTIKSGIQRFGANLNRYAGKEVP
                     TVVFPFLDPEPNKPAKWPHYLIGVGIAVLTLTGLWYKFPRDPQRLLIFLYLAGSLAML
                     LMWPSQWFGSRFITPLVPLILLFSMKGLQDFSSLIVKRTGMRTFSYVPCLALLAVPFF
                     IGIKPSRYNIGSLRDAHKYAQSKYPPAYQNFFNLGKWAKTNLPAQSVVASRKPTLFYY
                     TYQGPSCYYPTNSKTTVEFMNGLRESRAGYLVMEELGYKTTPYLSFFINNLPQHFQLL
                     HTIPNPDTKIYRILYPQ"
     gene            complement(45269..45982)
                     /locus_tag="PKM27_01335"
     CDS             complement(45269..45982)
                     /locus_tag="PKM27_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR05923.1"
                     /translation="MNKLSVLVPVYNEEKTIGRILDKLREVQLIGGLAMEVIVVNDKS
                     TDHTQEVILGYLEKNNPDNIFYYSHEKNAGKGAALHTGIAKATGDFITIQDADLEYDP
                     QEFNLLLAPMVRGHADVVYGSRFIGGRPHRLLFFWHTLGNKFLTFLSNLFNNMNFTDM
                     ETCYKMFRREVLQSLKLKEKRFGFEPEVTAKISKIQGIRIYEVGISYYGRTYEEGKKI
                     NWKDGLKAIYCIFKYKFFD"
     gene            complement(46140..48086)
                     /gene="gyrB"
                     /locus_tag="PKM27_01340"
     CDS             complement(46140..48086)
                     /gene="gyrB"
                     /locus_tag="PKM27_01340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNR05924.1"
                     /translation="MTQKAGEYTAKNIQALEGLEAVRKRPGMYIGSTDTKGLHHLVWE
                     VIDNSIDEHLAGHCNEIVMTIHPDNSITVKDNGRGIPTGYHEQLKKSALEVVLTVLHA
                     GGKFDKDTYKVSGGLHGVGVSCVNALSEYLRAEIRREGKIWVQEYSKGKPQTEVIVTG
                     ESSETGTIITFKPDSGIFEVLEYKRDTLANRLHELSFLNPGLKMTLIDEREQDDDGQP
                     IREDFLSSGGLSEFVTYLDEGRTPLIDKPISVRGKEDNIEIDVALQYNDGYNENIYSF
                     VNNINTREGGTHLRGFRMAVTKVFSEYGESNGYFSKLKFKVSGEDFREGLTAVVSVKI
                     PDPQFKGQTKGELGNNEVTTVVSRAVGDALKNHLELNPGEAKRIMDKVVLAARAREAA
                     RAARELIIGKKGISSISLPDKFADCSSRRPDECEIYLVEGESAGGTAKTGRDRNFQAV
                     LPLKGKILNVEKAFEHKIYESEEIKNIFNVLGVRIEEKDGERRLNVDKLRFHKVVIMC
                     DADIDGSHIVTLILTFFFRYMRSLIEHGYIYIAAPPLYLVSRGKQQQYCWTEDDRKKA
                     VETLAKGGNESSVKIQRYKGLGEMNADQLWSTTMDPKERLLKKVSIEDAVAADRTFSM
                     LMGSEVEPRRQFIEANAKYAKVDI"
     gene            48234..48653
                     /gene="tsaE"
                     /locus_tag="PKM27_01345"
     CDS             48234..48653
                     /gene="tsaE"
                     /locus_tag="PKM27_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNR05925.1"
                     /translation="MKKTFVYAKESLEDFVKTLIFQYPLNKKWAWYGQMGSGKTTLTK
                     SFIRQLGSVDPGSSPTFSIANTYLSATGESIVHLDLYRLHSGQEAFNAGIYELLQEPG
                     YCFVEWPERIENWLDEEWVTARIRILDGDCRLVDVFS"
     gene            complement(48656..49453)
                     /locus_tag="PKM27_01350"
     CDS             complement(48656..49453)
                     /locus_tag="PKM27_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNR05926.1"
                     /translation="MKKILVPVDFSKGTRQTLDFAIQTAEVLGSKILVLNVIDFMEKT
                     VGLRSVNQIIQQDAISRLEKFVAPYKKKSSIQILTKIAKGEPYTVISRQAEISGVDLI
                     IMGAQGDDNEENYFLGKVAGSIIKFKNIPVIVIPKSYQARKVQNIFFMFRKLNKSLFD
                     ILPPLFELSDAYKVPVKAVHLTGQGTGTIAYSPELIFENRAFTFEDLQADSFTEGIAM
                     IQARESMDMLCVIKRKRGLLEYIFESSPTPKTVIDSPVPVLFLNDDE"
     gene            49546..49842
                     /locus_tag="PKM27_01355"
     CDS             49546..49842
                     /locus_tag="PKM27_01355"
                     /inference="COORDINATES: protein motif:HMM:NF021758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HNR05927.1"
                     /translation="MARHTITIEYCPKCNWMLRAAYFAQEFLSSFTENIQGLTLVPSE
                     QNGSFIISVDGIKIFDRIESGGFPEIKVLKQMVRDRVAPHQPLGHADTPSRLKS"
     gene            49846..50442
                     /locus_tag="PKM27_01360"
     CDS             49846..50442
                     /locus_tag="PKM27_01360"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNR05928.1"
                     /translation="MKAVILCGGLSRRMGTNKALMHWKGKRLAQYGIDLVTALNLPVF
                     LSVKQADRDTFQKVFQGCGIIPDENGLSLEGPARGILSAHLLLPQEDWMVLSCDMPLL
                     TPSMGRDLLRSHGVHPEAEVSLFSSQGIYEPLFGIYTSTALGHWLRQDETGAWSSSSM
                     KYLLSRVRVRAIPAKDRQIPFFLNANRPSDLDAGVREV"
     gene            complement(50410..51897)
                     /locus_tag="PKM27_01365"
     CDS             complement(50410..51897)
                     /locus_tag="PKM27_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05929.1"
                     /translation="MNLTGIFKNYVQFKINGPGGIWPSGLLVTLTGILKEVKTERLKF
                     GLRQSMHFRVHKIYCDYCCRQLEKAGILYEADSDAYPNVMSSYPAAGIFSPASWLTEG
                     VYRDILDELQFRPRLKINICDIHQSFTPFFTGNVNWISSAIPHYWHLYIRFPGTNSVY
                     AWKELFYTNELMRFSRQLDSRMSDFRPVNEVQCKVAGEQLQLVMKSRGGTTIPKEAEP
                     DFPPFTLSYYEGFNAFQDHYWLGIYRRDEFFDVGFLHELGLLSQETRIGQICSTAWKS
                     LIIKGIEDKDRPKWIRLLNRYQINLRHALNELNFQVEDDSPSGLALKHFLVKRFNDAD
                     IRTTGICFGIKTRSKAEIFSSILIRKKYLFHLLGLDFIPVYDIFAAKDFNPNERSEYL
                     IGKSLFRYLVPDQLIACIRSYYRFRLEDPLWQEKLIPGAGVDSSDAGQPEYFFQCRQC
                     LSLAEFIPPEVLPWGYRCSVCEAPPGEFELKKKRELYFPDTGIQV"
     gene            complement(51894..52403)
                     /locus_tag="PKM27_01370"
     CDS             complement(51894..52403)
                     /locus_tag="PKM27_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase associated protein"
                     /protein_id="HNR05930.1"
                     /translation="MKNSESKPIQSFNPDFVVFEFEKEFVEEQIRCIPMIIRFKLDIA
                     GIKLSLAQWSSFAPEERNLLATLPCNHRLEKERYEASLQSLLKRHGWGEAERLDGLVN
                     AQLGPDLYDLVNSRANGLGHRIEPEQWEGLSVLQRFALGKLIRPGHESKNFVPALREF
                     GLMHQTLHP"
     gene            complement(52400..55963)
                     /locus_tag="PKM27_01375"
     CDS             complement(52400..55963)
                     /locus_tag="PKM27_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNR05931.1"
                     /translation="MTRAPHTGPQKYQSTCCYCGVGCGLVIRKEGENRIQLEGDPGHP
                     VNRGMLCSKGKNLHYTANDKSDRLLFPEMRYHRNLPRQRVSWDLALSRAAAVFKSLTG
                     KFGPDAVAFYASGQCLTEEYYVINKLMKGFIGSNNLDTNSRLCMSSAVVAYKMSLGED
                     CVPVSYDDLEACDVIFVAGANPAWCHPILWRRVERAKEKNPALTVIVTDPRKTQSCDL
                     ADLHLQIRPGTDITLHHALGRALIELGYIDTSFIQNHARGFEQYRESVFKRSLEEAAR
                     ICGLNPEDIRQAAIRIGRARAFLSMWTMGLNQSVSGVNKNLSLINLHLITGQIGKPGT
                     GPFSLTGQPNAMGGREVGGLCNLLPAHRDLLREDHRNEVERFWKIPPGTIAPKPGLTA
                     TEMFDALDTGALKAVWIIATNPLVSLPDVRKAEAALKKARFVVVQEISNKAETLEYAD
                     LILPAAAWAEKEGTMTNAGRHIGLLEKVLDPPGEALPDAEIICRLARKMGFHGFDFSS
                     SSQIFEEHAELTAGTRIDISGLDYNILKKQRTVQWPYPKKLLEDNAGFPGTERLFQDQ
                     VFYTADGKAHIHACDEENHSEPLSRDFPLILTTGRIRDQWHTRSKTSKVNKLNQHFRE
                     AFVEIHPLDALARGIEEGKLVEVINHRGQARVKAKLSPELRQGVVFMPMHWGKAGNQD
                     LLRANNLTAKLLDPVSKEPDFKYAAVNVRVWNKPVQKIILIGAGAAAFGFLKSYRALN
                     RQDRIEIFGDENHPFYDRVLLPGYITGALPWSSLVKMDREEENIPHTVFHRGLAITAI
                     DKFNKSVTDAQGQKHDYDLLVIATGSRAATIRNLPPMEGIFTMRSRRDADAFKSFVDV
                     AEETVVILGGGLLGIELAASLKEKGARTVIIQRVSRLMDRQLDPLGSQVLHEALTERG
                     IDIYYNDEIERYLGEKRVTGVRLKSGLTLECKSVILAIGTLPNDQLARDCGLECQRGL
                     VVDEYLRTSDENIYAIGEVAEFRGISYGSTAAAEHQAQILAGWVSGDTGSIYRGSLFM
                     NILKIQGLDLCSLGDALIPPGSEGCEEIVFMDLAKRYYKKCIVRDDRLVGAILIGDKS
                     EFLEFRDLIAGRMELSEKRMDLLRSGKKQEPVSGRLVCSCNQVGEGNLEEKMNAGCTD
                     LVQLCQLTGAGTGCGSCRPEIKNLLKAHLKAREQHHTPLCI"
     gene            complement(55966..57261)
                     /locus_tag="PKM27_01380"
     CDS             complement(55966..57261)
                     /locus_tag="PKM27_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HNR05932.1"
                     /translation="MSQKSLSVLNLLSFKGVQMRTFHITWLTFFVCFFGWFGLAPLMP
                     AIKADLNLDKSQIGNIVIASVSGTIVARLLIGRLCDTWGPRKTYTALLLLGALPVMAV
                     GLARDYSSFLLFRLAISIIGASFVITQYHTSMMFDDRIKGTANAVAGGWGNLGGGITN
                     MVMPLIFALLVGFGFTDKEAWRYAMIFPGLLMLVMAFFYYHYTQDSPAGNYEDLERRK
                     PGKIKTDYSVLKDWRVWALTLAYAVCFGMEITFDNVAALHFVQEYDLSQQSAGFWAGM
                     FGFMNLFARALGGIFADKAGRLYGLRGKGLLLSGVLMFEGLGLIFFAHAVSFSLAILS
                     MIGFALFLKMANGVTYAITPFVKPAQVGMVSGIVGAGGNLGGMVFGFLFKSRSLTYLD
                     AFNYIGMTVMAVAFLVFLTRFARPLTEPRLSMELSPNVL"
     gene            complement(57272..58840)
                     /locus_tag="PKM27_01385"
     CDS             complement(57272..58840)
                     /locus_tag="PKM27_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNR05933.1"
                     /translation="MYCKKAIASFACAAVLLFGPLSVPAQFSIAGQLRTRSEIRHGYG
                     NLLPSGSRPAGFTSQRTRLQMNYQNSGIGIGASVQDIRVWGQDGSTIAPADGSRLMLH
                     EGWAEILLTKTMDSLDGPPLIGYVKLKLGRQELSYDDQKLIGNLDWLQQGRRHDMALL
                     KLGIKKWQIEIGQAFNQNAEGFRATSYVPGSVPATIKNAAGTPVSVPAGLVPLTLGGQ
                     VNALSTPSGLPVYIHPSGTNAPSQHYKSFSSLYFSRKSKLSTWSFLFFRDRFTRYKLD
                     SIILDGAYLYGRRFIKSGDQDDFDYRTLSRTTIGFTISQHLGRISGLGWVGLQAAIYH
                     QTGRDRDDLRLKAWHCSFSTSLQWGPLHIQAGYDALSGDDDHTRPGHSHRFDPLYGTP
                     HRHWGYMDFFYVATGSPLAGLQDGYLKIKLVSKKENLSTGLDLHRFHTAHHFPDDRGN
                     TMKKYLGLELDWITTFSLNPYTQIESGFCYYRASPSLQYAKGLPPGTYQSAPAWAYVQ
                     FNIRPGFFLQKSTP"
     gene            complement(58918..59292)
                     /gene="nirD"
                     /locus_tag="PKM27_01390"
     CDS             complement(58918..59292)
                     /gene="nirD"
                     /locus_tag="PKM27_01390"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNR05934.1"
                     /translation="MEIGESAEPGDSGRTDEHWFFACYKEEVPEDGGVCIKYGDCQIA
                     LFYFARRNEWYATQNECPHRKQMALSRGLIGSAGDEPKVACPFHKNTFSLKSGINLNG
                     NEQSLEVYPVRLDENKVYIKIK"
     gene            complement(59283..61775)
                     /gene="nirB"
                     /locus_tag="PKM27_01395"
     CDS             complement(59283..61775)
                     /gene="nirB"
                     /locus_tag="PKM27_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNR05935.1"
                     /translation="MKIVIVGNGMVGFKCCEKLTGRAPGRFQITVFGEETRPAYDRVH
                     LSDYFSGKEEASLYLAPAEWYAGQGIQLHLGDPVCQINRQSKTVDSFKGKSVPYDLLI
                     LATGSAPFVPVIEGIEKKGVYLYRNLEDLDLIESRAAKSQKGAVIGGGLLGLEAAKAL
                     LDLGLREVSVIEYASRLMPRQLDEMGSQILMEKLKTLGLNILLGKNTVALEGDPGIRQ
                     LLFSDGTGLDTDLLVISAGIRPRDELAQMAGLETGTRGGIVVDENMQTSDPFIYAVGE
                     CALHKGMIYGLVAPGYDMAEAACCHILDQPKPFAGYDMSTRLKLIGVDVASFGDPFAN
                     GSQADVITIHHGPEGVYKRINISKDGKNLLGGILIGDASQYNLLVQTVRNKVILPAQP
                     EDLILGDRGNAVSGGLGSLPDEALICSCESISKKSICDAVRFEKCENVESIKRCTKAG
                     TGCGGCVPILKDLMTETLKAEGKYIRNRICEHFEYSRQELFDLVKLYQIKTCNEVLSR
                     FGSQDGCEICKPVIASILASLWNEMPLRSGNGTAQDSNDRFLANIQKGGSYSVVPRIP
                     GGEITPDKLVAIGLTAKKYNLYTKITGGQRIDLFGAHLHDLPLIWEDLIAAGFESGHA
                     YGKALRTVKSCVGSTWCRFGLHDSVSFAIRIEERYRGLRAPHKIKSAVSGCIRECAEA
                     QSKDFGLIATEKGWNLYVCGNGGSKPQHALLLATDLDEQTCIRYIDRFLMFYIQTADP
                     LTRTATWLNKMEGGIDYLRNVVIRDSLGLAAQWEQEMDQHVASYQCEWKVAIETPEIR
                     KRFTHFVNAPGEKDPTLDFEPMREQKKAREWK"
     gene            complement(62412..63671)
                     /locus_tag="PKM27_01400"
     CDS             complement(62412..63671)
                     /locus_tag="PKM27_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNR05936.1"
                     /translation="MIHPSIPQAVYAGDDLVGSDIAWMICASALVLIMTPGLAFFYGG
                     MVRKKNVISTMLQSFISMSVISIIWVVVGFSLAFGDSIGGIIGNPATYFMMDDILAQK
                     PWSLAPTIPLVLFCFYQLKFAIITPALITGAFAERIRFSSYVLFIILWSLLIYSPLAH
                     ITWHPEGLFFGLGVLDFAGGTVVHMSAGWAALAAALFLRRRSEATGLEPARITYVMLG
                     TGLLWFGWLGFNAGSAMGANSLAATAMATTTAASAAAAFSWIIFESIRGRKPSAMGAC
                     IGAVVGLVAITPAAGFVSIPHALTIGIVASIVSNLVVEWRSRTTLDDTLDVFPCHGVG
                     GMVGMILTGVFASQAINPANTTGNGLFFGETKLFFIHLLALIAVSIFAFFGTYILLRI
                     TNAISPLRVHDEEEFQGLDLSQHGEKL"
     gene            complement(63973..64290)
                     /locus_tag="PKM27_01405"
     CDS             complement(63973..64290)
                     /locus_tag="PKM27_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05937.1"
                     /translation="MEKDDRLPEQWMTDLEGQKPFLDEAAFLSLLEDRVSQLLADQPD
                     LLWSFLYRLDVEESKIRKALQSQADAAHALALLILDRQKQRVATKKAYREGSGRAFNP
                     PDL"
     gene            complement(64277..65779)
                     /locus_tag="PKM27_01410"
     CDS             complement(64277..65779)
                     /locus_tag="PKM27_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNR05938.1"
                     /translation="MLEFFLSRLFRNRYRQVESWMKEPIRFQNELLQDILYKGNYTEF
                     GRNHHFSAIRDYESFIRQVPVRNYPEFQPWIERMRKREENVLWPGLVDMFSKSSGTTS
                     GKSKYIPVTHEYLEFNHIQGASDTLSILYNQFPNLGLFEGKNMIIGGSLERLAEYPGI
                     VSGDISALLLNNMPWYARQVFVPSIELATAQDWEFKLAETPGQILDEPLVMFAGVPTW
                     LIVLFKNILQQTGKENLLQIWPGLKLYIHGGVNFGPVLPVFKKLIPSESFLYQEVYNA
                     SEGYFATRYNQQGEGMLLLLNHAVFYEFKDLHSGNIVPVEGIRLNTPYHLIITNNCGL
                     WRYDTGDVIECTGLDPVRIKIAGRTTQFINAFGEEVIVENAEKALEKACGEHQCMVTD
                     FTAGPKYMDGGGKGAHEWIIEFEKSPSDLKAFARSLDLALQSLNSDYEAKRTKNLALE
                     QLIIHEMPRGFFYSWMKSQGKLGGQHKVPRLKNDRSIIDAVLQFYNHGKG"
     gene            complement(65966..67126)
                     /locus_tag="PKM27_01415"
     CDS             complement(65966..67126)
                     /locus_tag="PKM27_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR05939.1"
                     /translation="MRYLTVVCLVFFVWTGLRSEGIVFHQGTWPEALAKAKESGQLIF
                     VDAYTTWCGPCKLMSAKTFPDKSVGEFYNTHFLNVKLDMEKGEGLDFSDKYGVSAFPT
                     LLYIDGEGQLVMKAVGYMDPAKFLDAGRSALKKGDKSALYAKEYDAGKRDFNTVYNYV
                     KSLNQAGKPSLKIANDYLNSQKDLTTPDNLRFILEAATETDSRIFSLAVKSKEALVKL
                     FGEEKVKSRWIQAGHRTVLKAVEFQSFDLLTGAQQEIRAAMPEEGDAFEFNSNIHYYT
                     SVKDGESLYKAMKKLPASVEKDPGLINHLSLEVEKSFNTDEKLTGLCEQLLSKLINSS
                     SAVEHQFTLARIYALNGKAEKASKLLSQLIPLAKTKGMDTVPMEQFRMKLGS"
     gene            67125..67256
                     /locus_tag="PKM27_01420"
     CDS             67125..67256
                     /locus_tag="PKM27_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05940.1"
                     /translation="MMNRLVCKNKEKRFEKKVKVEAEAEVEVEVEAEAEAEELTTDY"
     gene            complement(67275..68123)
                     /locus_tag="PKM27_01425"
     CDS             complement(67275..68123)
                     /locus_tag="PKM27_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR05941.1"
                     /translation="MNAVMITGVSTGIGLATAKYLKGFGFHVFGSVRKPEDAVKLEKE
                     MGPGFTPLIFDVRDRRAIDVAAAQVAEMIGGQYLRALINNSGIAVSGPVQHVSIDKFR
                     EQLEVNVIGLVQVTQAFLPLLGAVKPQTGKPGKIINISSVGGRLSRPFYGPYNASKHA
                     VEGLTGSLRRELLDFGIDAIVIEPGPIVSEMYEKAKYDTDTFEGTIYHDLYKHKNKFI
                     EFSQKMSIPAEEVARLIHNSIMMDKPKTRQVIVAKKWMIELMMKLPDRVVDKMMLKQM
                     KGVLEK"
     gene            complement(68130..70277)
                     /locus_tag="PKM27_01430"
     CDS             complement(68130..70277)
                     /locus_tag="PKM27_01430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNR05942.1"
                     /translation="MKKILVPLILSFWTCFAMAQPEIQVSDLTRIQSVSNVSYSPEGD
                     RILFTVTSIKPDPRDPWEYQYGTQLWIGDAQGNGLRPLTWDGASSPSWSPDGKNIAFV
                     RGIEGKAQVFILPLEGGEAWQLTREKNGAANPVWSPDGSYIVFNSLFTLADLAADSTL
                     NAGNSFPEWPMEKAGFRDNAYILPSKTKANPNGTLDEIRTYLALNEKDRKAKVINKLV
                     FQQEATTSNDLSFSHLFRIEVKAGAKARAISSGFYSFSNAGFIGNSPQLLVQTDLDQK
                     EHPDRSLESEVYTLSQDGNLQKLLGKDGLSFGQAQASPDGKWMAFTRGETNRVGIPAL
                     AIMPVRGKERDIKVIPHDRTVGNLKFSPDGTSLYFTSPSNGGVVLYRLRLEDLKKEQL
                     SGFDEGIGSFDVSRDNQLAFVKTRVSSPYELFISDRSLKNQKPVTTLNTSWLKDKHIS
                     YPEKFTFTNDQGMNIEYWVMKPVGFAAGKQYPVVLNIHGGPSAMWGPGEASMWHEFQY
                     WCGKGYGLVYCNPRGSGGYGQEFLRANIKDWGKGPTDDVLKALDGAASQGWIDTSRMV
                     VTGGSYAGYLVAWIIGHDHRFKAACSQRGVYDLKTFFGEGNAWRLVPGYFGGYPWEPA
                     TRDILERESTINYVQQVGTPLIIFHGEQDLRTGVIQSEQFYKSLKVMGKTVEYVRHPG
                     ATHELTRSGNNRQRIDQMLRTWEFFERFIHPNK"
     gene            complement(70313..71065)
                     /locus_tag="PKM27_01435"
     CDS             complement(70313..71065)
                     /locus_tag="PKM27_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR05943.1"
                     /translation="MRVILAEDEITASRKLAHLLLKADPSIEIVASPESVKAVVDWLR
                     HHEPPDLAFFDVRLSDALSFEIFDRCKIGFPVIFVTAYDDYLLKAFDHNAVHYLLKPV
                     TEEKLKKALQKVSQLKTHFLSGMDHFLKDRKVNATRILVHKGAYLIPLDASGIAYAFT
                     EHKICFVRSGEGDLYVADQTLTELEERLDPVLFFRANRQYLVNRNFIVRFKSIEQSKI
                     LLELKPSARQEVIIGKQNAVGFRKWIKDTGLH"
     gene            complement(71065..72126)
                     /locus_tag="PKM27_01440"
     CDS             complement(71065..72126)
                     /locus_tag="PKM27_01440"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNR05944.1"
                     /translation="MSNKWKSSDARLILDDTVVRLIGVPFFGLVIPSATGLIDRAALP
                     VHLILIYYLWFVFTAWMVWEGNRYLLFRYHTTILQSQSLLQKYLTMIGLNLFYTIPAA
                     LLLLFGWKWAAGADDLPPRQIYLTVAVIAICVIFVSNMYEKVLLVKRTEQEKLVAEQL
                     QRSKIQAELEALKNQIDPHFMFNALNSLSFLIENDANKARQYTENLAEVYRYILQSKD
                     KDVVLLQDEIDFMNLYVSLLELRYEDAFQVHFGFDPRAQGQTYLIPPVSMMIAVENAV
                     KHNELSKTSRLVLDIDCREDELIISNKMRVRKQGYPSTSTGLRNLDERFDKLLGKSIR
                     SFREGGRFILCMPLLKLNG"
     gene            72272..72679
                     /locus_tag="PKM27_01445"
     CDS             72272..72679
                     /locus_tag="PKM27_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNR05945.1"
                     /translation="MEPIQINHLAVLVAALSTFLLGGLWWSPVLFEKAWKEENRFTDE
                     MLRQGNMAKIFGLAFVFSLIMSYNLAYFLGDPGIKAGTGALYGFFTGFGWVSMAIFII
                     GLFERKSWRYMLINGGYMVVAFTIMGLILGAWK"
     gene            72820..73713
                     /locus_tag="PKM27_01450"
     CDS             72820..73713
                     /locus_tag="PKM27_01450"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HNR05946.1"
                     /translation="MNKAALLFFLLLSVRFPAGAQTPFSGDPDQSLFLTTDIDHFWTA
                     YDLFVKDTSVNPFGPHYLEVGSKGVKGFTPFRIRSADYLYNTVKRHRADYDSIRQNTL
                     RIHETEKQCRSAFYALKYWYPEAVYPPVYFVIGTFNSGGTFNKNGLFIGAEMQKEIGN
                     IPHIVAHELIHFQQKNWVNQPNLLRQSLVEGSADFIGELISGKHINPAAHAYGDRHLE
                     NLCREFVARMDSTDYQDWLYGVSGKDDRPNDLGYWMGYKITQQYFNKSADKPQAIRDI
                     LDIKDPLKFLRASGFLDPYLE"
     gene            complement(73858..74925)
                     /locus_tag="PKM27_01455"
     CDS             complement(73858..74925)
                     /locus_tag="PKM27_01455"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNR05947.1"
                     /translation="MPFSYFNSVDETIGNTPIIKLKTISEQVGSNVYAKFESYNPGHS
                     AKDRIALYIIDKAEKEGKLRPGGTIIECTSGNTGRALAMVGGHRGYKCIFTVPNKISE
                     EKKRILVALGAEVHVCPASVKPEDPRSYYSQAQFFADSIPNSYFVNQYYNMDNVDAHY
                     HLTAPEIWTQTNGAITHFLACSGSGGTLSGISRYLKERNPNIKVIGIDAYGSVLKKYF
                     EEGIFDESIIKPYALEGVGKNIIPMTFITEHLDQMVQVKDQLAIKRAIKLTAEEGLMI
                     GPSGGAAIQGVFEIRKDLKPTDQILILVPDHGSLYLSKLYDDEWMNENVYHKPKKNKY
                     GLPSYDKMKKLLNGIYESYKQ"
     gene            complement(75114..75542)
                     /locus_tag="PKM27_01460"
     CDS             complement(75114..75542)
                     /locus_tag="PKM27_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05948.1"
                     /translation="MKQLLMTLVCSLLLTTGALYGQSCHSKSAMSGCCKKSSAAAASL
                     VASQDPSIEVRKDVATGEVSYFRKKMCSYGGHVSYAALNFDAKSNAFVNQPPAVSSEA
                     QLLRVSNESGQKAKMDCSKMSKAECLERMARGECTDKAKT"
     gene            75704..76117
                     /locus_tag="PKM27_01465"
     CDS             75704..76117
                     /locus_tag="PKM27_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05949.1"
                     /translation="MTALLMALLPGTHTAQKFKPEKYKKAILYFGHGGGFAGAETTYA
                     LMENGRLYSKKSLTAKTFTSLIKLEKNQSAQIFNNYFFLGIPEMQLEDPGNIYKFVEY
                     TRKQNRHRLVWGGQSPVPTNLVLFYSNLIGLLPKP"
BASE COUNT        19199 a        18842 c        17887 g        20191 t
ORIGIN      
        1 aatatccgga aaccattggg atcggatcgc actacttcgc ggtccaggcg gtcgtaggta
       61 taggtcgtag tgcccccgga aggtattttt ttgatggaca agcggttgga ggcatcgtag
      121 gtgtaaataa aactgccatt ctttaccgaa gtggaggtat cgaccttggt ggcgtcgcta
      181 tgcccgctgg ctgctgctaa aggttggatg acctggacca ggttttgacg gtcatcgtag
      241 aggtaatagg ttttgacggt atttcgggct tccatgacct tcaagcccag gagatccgta
      301 taggtgactg tggaatcccc gttttcattg atgactttta ctttattcag gctattgacg
      361 gcataccagg cactggcggt ccggaagttc ctaacctcat tgctgccggt attgcaggag
      421 tatacgtaga cgacaggttt ggtattgaaa acgctgcctg gatgagtgac cttattaacc
      481 cggctgagcg ggctgttttc atattcggtc accgttttgg cataagtccc ttcggagcct
      541 ccaaactgat tattataaaa ggtcactaat tcgctggaag cggtgctcag ataattgcca
      601 ggggtgccat tggtatacgt aaacggaaga taaccattgg gttcccggcc gtaactgtcg
      661 tagtcatggt gcaggatcac atccttggaa gtaggagatt gtcctactcc gatgttctgc
      721 aaagcccggc ctatatcatc gaaatagttg aattgtctcc ggacattgga tcctgacagg
      781 ccattgacgg ttccggtaga tgtcacggca tcctgtaata catccctggt ttttaccgaa
      841 ttataggtac cggtaccggt cttataagtg tattcatggg ttttcaaata gtatgagtta
      901 taatttttta ctccggtcat ccggagcaat tcatcgtatt caaattttcc aggtatgctg
      961 ttttcgttgg ccatacccga tgccaggagg gtatttctat tataactata ggttgtcatc
     1021 agggcatcta cgggatatag ccttaactcg tccactgcga caaatggatc atcgactgtc
     1081 agggtaaggg tggacgctga attcaacgtc agcgtatgtt ggacgaatcg ccagccatta
     1141 gcatcggctg aggagatcac atacgtattt gttgtagcgc cggaaatacc ggcctgggta
     1201 gcctggtctt taatccaata actgagaaaa tattttcctg ccggatgact ggacttgctg
     1261 atatttccag tcccggtcca atagccgctg ccggttttgc catccgcatc cgcttgcagg
     1321 ctgcctgtat tgctgatggt ccagcctccc tgacttgcct ctgtcgtaca aaatccggta
     1381 aacgcgcatt cgcttttcaa ggcattttga accctggcgg ataaaagcgt ttcattatgg
     1441 ctgtaaatca aggtgttgac ctggtcatat tccttatgcg cctcctggac attagtccca
     1501 gcagggtggt atcgatcgat ggtctccctg agcacatcac ttccacccgt ggaaaagttt
     1561 tcataaaaac ggtaaggcaa tacccaggcg ccggtcccgg gtaagccggt ggatgggtca
     1621 tattggttga aggtgatctt ggttctgtac acccttacat tatctacata attttcattt
     1681 tcaagtggga tgccgatcat gttttttcct atcatatcgg attgggacac atcattggca
     1741 aatttagtca cccttttata cactttagta tcgctgttag taaccacaga ctctgtgggc
     1801 gatgaaatat tggtattccc gtaagtatgt gtaattgttt ttttaaccga accggcaaga
     1861 gccgcaccgg tgtaataaaa aacggtatca aaaacttcct tttgatagat ccagtcctgg
     1921 gtgatgagat aatgctttct ttcccattta ctttgataaa ccgcggattg agaacaattg
     1981 ttgttatctc cgtcgattac ccaggtgtac actccgccat tcaggcatag ttttagttta
     2041 ttgtcctgca gctgtttagc ctctacgatc atcccgtaaa aactctgaga ccggcgcgtt
     2101 tcactgatat cgaacgaata tttatatccg gtagagtgta attttttacc agcatcatca
     2161 tattcttcaa tcagctttag ctgtccaagg tcctggatgt caaattcatt gaccaaattc
     2221 caggaattgg tgccgggcgc gtagttctta taatggtaaa cggttttccc ggcaaagacc
     2281 tcctccaccc ggctataacc aaccagactg ccttgaatgg atcctaacgg gactcttgat
     2341 gaagcaaaat acatgtagct gacacagtat ctgttgatcg cctgctggga gcaaagttga
     2401 gctatggacc atgtataggt agtaaatgtg gaagaagtat aatagtcagg gtgatagaat
     2461 agaattccac tgcaattacc attactttga atataattat attgtttgtt ggtaagcaga
     2521 gtaccgggag ctgcatacgt tttaatgttt tttattctaa ggccccctac caaccgttct
     2581 gtattattcg tattgcagat gaaatcggaa gtaatcgctc ggtttccttc ccaggtaaac
     2641 tcagtataac ccctggttgg aaatacaatt ttaataagtg tacctgcctg ggcatatgtt
     2701 tcattgggtt ccctgtttgc accggatgta gggttatcca tgccggggca actaacatcg
     2761 tttggattta cgccgtcaca atttattacg ggcagtagtt tgtttgtggg ccaggtgcca
     2821 ttagatgctc cattgtaaaa gccccaatga tcgattgaat tcgaggtaaa tgaagggaga
     2881 tccgtggcat tataataaaa agcatgggct tgtttgctta atgaagcgac agcattcctt
     2941 tctttaatgg aatccagggt cagcctgccg gtttggtctg aataataaaa ttgctggtgg
     3001 accaattcag taccggcatt gacacctctg taaaggctta ttttatccag tttcctccct
     3061 ccaccagtac cggtataagt attggaagat gaagcaaatt taagtttttc aattgccact
     3121 gttcctttct taaaaatgac ttcatccagg tatttcctgc cggcaatggt aaaggtgttt
     3181 accgatgctg cattgtcagt agaattgtca ccacatggac acgttgggtc cggacatcct
     3241 ccagattgat atccatggga gaaggtcctg gaccaattgg taccagggtg tacaaactgg
     3301 tttgggtcat aggcgctgcc ttccgagata tatttaatct cgacggtctc tgttgcattc
     3361 ggactgatca cttttgataa ataccaggaa gatttataca caaagttggt atggtacctg
     3421 ggccagttgt catcactggg gtaataaacg gtggtttgct cagtagctgt aaaccaataa
     3481 tttatgcctg atgcatcttt caccaggaaa ctgtcaatgt catcggtagt ggagttccag
     3541 taaggtgtaa ttgccaaatc tttgacttcg ctcatgacaa tcgttttgga tgccgtgaaa
     3601 taaaatttgg cgctttgtcc tccagcgttg aaatagaaaa tatcgggctg tgtttctatt
     3661 tctttggatg caacagattg caggaagttg ttttcatcga attgattaga tttttcactg
     3721 gaggaagcct gatttatggt agatgatttg ctgaaatagt ttactttcat atcgggatcc
     3781 ccctttacag atcttgaaat cactccacct ccattcaagc tccatccgac acctacccag
     3841 gtgggtactt gttctactct gattccacca ccatcgtatg ttaaactgaa tggcgcactc
     3901 acctcggctc ctttgagttc atacaaggga atggaaatgt ttggaattcc agtataaggg
     3961 cttattttat aatcaccaaa tttacccagg gatgcagctt ccggggccga aggaagagtg
     4021 accttatttt gcaaaaatga gacccgacga aatttaaaat cagaaggtcc atctccctgc
     4081 gcaccaagag atattcccgt aaacagaaaa aaggtaaacc agcttaaaaa aatgaaattt
     4141 ttcatatcgc tctctattga attcccatta attaatgata accagcctta atactttcaa
     4201 ttcctgatcg gcctggagac ggagataata aattccgctg ccgagattta agtcactaat
     4261 tttcaattga tattgacctg ccccctgcat tccaagattt aattgccggt aaatctttcc
     4321 ctcagggtgg atgatatcaa ttttgatttg gaccgcctga ttaaaggaat attcaagcac
     4381 cgaattctta aaggccgggt tagggtatag ggctgtaaat aattcaagcg aatttcttac
     4441 ttcgagttcc gaaatctttt tgttcgtatc cagccgccag agaccacctc cataagtgcc
     4501 cgcgtaatat tttcctttat gataatgaac tactttggca cgcaaatcag atttgccgta
     4561 aaccatttta tcaccctgat ttagaatatt tgtatttgcg ctagccacta ataatcccgt
     4621 agaatcactg accagagagg taacattctt caactctgtc tccgatttat ttggaagttt
     4681 cttttgccaa agctcaccat ctacggaggc caatatccca tcccccgaag aacctgccag
     4741 cagggtatcc ttgttttcaa aaaaggcttg gatatcaaaa acagtaaaag cctcctggct
     4801 tctccaggat atgccatgat cttcgctgac aaaaatgtac cggccggatc ctgcatagat
     4861 tttgttttta tgcgtccgga aagtaaatgc tatggagtgt tttttgtctc ctttgatttc
     4921 cgccttaatc cattctaatc catcctggct gtaataaatt ccgctccacg tacctgctat
     4981 taagtttttc cctgagacgg caaggctata gatataagga tcgggggttg aagaaatgaa
     5041 ctcccaactt tctccttcgt taacggattt gtatattccg tccccatatg ccgccgcata
     5101 aagagtcttg ctattattga taaggctaaa aatgaccgac ttgcttaagc cggatttaat
     5161 ttcagaccaa ttgtccccat tattaatcga tttataaatt ccgcctccca tggtggacac
     5221 catcaggtct ttgcccagac ttaaaatgtt tgaaaccggg tattgctcct gaaggcttgt
     5281 ttttgtccag gtggtttgtc cagaaatggg aaatgaaatg aactggcaaa aaatagccag
     5341 tatgaaaaaa gcatctttca tttagtttaa attgaatgag gttaaatgga atgaattttt
     5401 ttgtcttgga tatagaattt tgatcagatt gtttttacgg acatatatgt ttaatctcca
     5461 aattcatatc aaatgaatgg gatgaacaac aaataattgt ggatttgtaa gtaggaaatt
     5521 cagattataa aatgatgctt tttagtaaat ctttaattta agattccaga gtatatgtaa
     5581 gaagtcggcg aaatgtcgtc ccttccttaa agaaattttt tgggccaaaa agggacaaat
     5641 caaggattaa ttaagaaaat gattacctac ccagcccgtc tctccactat ccggtcggga
     5701 ggatcagctc caaagaacac atggatctaa taaaatacct tatatgcaag gagtcgttcc
     5761 tgaagcataa actctgggac cgctcccaaa gctgaagaat ggggtctaca acaacttaaa
     5821 ctccggcaaa accatcaccc tcatcccggc gtccacggca gcctggatcc ccagggggcc
     5881 gtcttcaaag accaggcagt ctgcagggtc gatgccgatc aaacgggcgc aggtcaaaaa
     5941 ggtctcgggg tggggtttgg gatggaggac gtcatcggcg ctgactaccg tggggatcag
     6001 gtggtccagc cccagggccc ggatcacgcg atccgcattg accttgctgc tgcccgtacc
     6061 gatggccatc ggcagcctgc cgtggtaatg ttttacgagt tcatacactt cggcgatggg
     6121 ttggatctct tcgagctcat cgagcaggga caggtagtaa tcgcttttaa tggttttgcc
     6181 cgccagcgga tcaatgtccc agccataacg tttattgaag gtcttgatca cctggtaaga
     6241 gggcgccccg gccatttcat tgatcatttc cgcagtgatg tccaccccaa aatgcctgcc
     6301 cgtcagggac caggccttga cgtggctcgg catggtatcc gccagggtgc cgtcgaggtc
     6361 aaaaatcagt cccctcacgt cttcggggac caatgaataa atttccatag ggatcgcttg
     6421 ttgccggcag gctgtggaaa taaacacaag gcgtgcctcg tgcaacacag gccctcgcct
     6481 tcactgtccg gctgtttaat aaaatcaggc cttactgccc gccttgacca aatttgtaaa
     6541 actggttttc agattttcca ggggttttgg ggccgcatcc tgctccacca ggaaaaattg
     6601 cgtaccggcc agttttgcct ttttaaagat gggttcaaag tcgatgtaac cctgtccgac
     6661 ctctaccggt ttttgatctt tgtcaaggtc tttgacatgc cagagcggga atcgcccggg
     6721 atgagtcttg aacagttccg ggatattgac ccccgccttg gtcgcccagg ccaggtcgag
     6781 ctcaaacttg aggaggtccg cgctgatctg ggtggccatc atatcaaaag gccttttccc
     6841 ttccacgagc tcaaattcgg cggtatgatt atggaaagca aactggatgc ccgctttttt
     6901 gcaggcttct ccggtttttt gcagcacttc cagggattgg ttgatttctt ccatggtctt
     6961 caccggagta ctggagcata ccaggtattt gagtccgccc tgggccgctt cgtccaccag
     7021 ttcctgcata tgggtggcca gggaacggga gggggggcgg ggaggcgtgt tgggattatt
     7081 ggccgaagga ggagcgccgc ccggccttct gaaaggaaaa cctgaagcgt ggtgcgccac
     7141 ccacttaagg ccgtattctt tggccagttt ggcaaattcc ggagcgggca ggccataata
     7201 tccacccaat ggggaaccgg ccgattcaag atggacatat cctatatcgg ccaatccttt
     7261 cagcacccct ttgaggtcat cgttgatgac cgagttggcg gtgtataact gaacgccgta
     7321 tttccttttt tccttaatca ggcccagcac atcggaggga gccaccaggg tgccggcgga
     7381 caaggccagc gaagttttaa taaacgatct tctttgattc atgcctgcga aattaccgga
     7441 aattaaaaga aatagttagc aagttggcaa gtttgtaagt tagcaagttt gtaagttgga
     7501 ttattcactc tcctgcccct tgcctctcct caaattactt tgccatcagg caggcaagcc
     7561 tcaaggggag tcaaaccctt ggcccatttc tattaataat tttgatctcg ccttgtgctt
     7621 attctaaata agggcagagc ggaattgtcc ggtttaaaat tgcggccgag atctttggtt
     7681 ccccagcggc ttgccccact tacaggattt gagaattagt agtctggggg gacaggggca
     7741 aagtagcttt atatataacc tcccccaccc cctaaagggg agtcaaaccc ttgacactct
     7801 tgcatttatt cttttcatct cgctctgttt ttattcgtaa acttaattta ttcccctccc
     7861 cttgcctctc ctcaaattgc attgccattc ggcgggcagg cctaaagggg agtcaaaccc
     7921 ttggcccttt tacattaata attttgatct cgccctgtgc ttattctaaa taagggtaga
     7981 gtggaattgt ccggtttaaa attgcggccg agatctttgg ttccccagcg gcttgcccca
     8041 cttacaggat ttgagaatta gcagtctggg gggacagggg caaagtagct ttgatatata
     8101 acctccccca ccccctaaag gggagtcaaa cccttgacaa tcttgcattt atttttttca
     8161 tctcgctctg tttttattcg aaacttaatt tattcccctc ccccgcccct cctcaaattg
     8221 cattgccatg aggcgggaag gccttgagga gagtcctacc ctcagcgagt aggcaactta
     8281 attggttgat agggaatcac ttcattcctt gaggcctttc accaccacat tgccggtgcc
     8341 gtttttatcg cagggataac ggacggcttc cgtccatttt ttttcattcc ggatgatctc
     8401 gaaaaaaccg ccgaggtatt ttttaatcgt ttccggagtg tttttttcct gccagggctg
     8461 gcggtccacg gtggccatga tttcgttttc gcggctggtg aattcagtca ggacttcacg
     8521 gatcaggctg tcgtccgcca tacaatatcc ccggtacctt cgctcggtca ccgacctcag
     8581 cccaagcgct tcggaaggtt tggcataggg cgcgtcgacc agtccggaat ggtcaaaatc
     8641 ataagggacc gggaccggtt tttccccggg tatggccagg agacggacat tgtgcaggta
     8701 ctgaacggac cagtcggtat tgccgatcat gtattcaaaa agggccatgc gtacaaagtc
     8761 acggggggcc gtgttttccg ggcgcatcat tctttttgtg atcagggtcg cctcatttct
     8821 cgccgccatg gcctcatctt cttcgagcag gtaaccggtg acgctaaagt cctttttaga
     8881 atttccctct tcccggaact gaatatgcac cattcgtacc ttataacttt tagggccgaa
     8941 aagattgtgc aatttgtaaa ccaggtactc ccggaccaca taatcttcgc cgcggcaggg
     9001 catgaccagt ttcagtttct tttttcccgc aaaaagcccc ttgtcaaccg gttgaccagt
     9061 gaagtccatc gacagtggcg ggtaggtgca attgccctgg gtgcgcctga aattgcccct
     9121 cgttttcatt ttcagcgaaa ggctttgcgg tccatcgggg ccttcgtaaa ccagggtcac
     9181 gggatgcgct ttcgctgcat cgctgcggtc ctgaatcagt ttgtttaccg ctccggtaat
     9241 ggtcagttga aggatggact cctcatcaaa aagcccggaa tcggcgaagg caaggggttc
     9301 accaatggaa cccatttctg tacctgtcgc attccaaggc aagaaataaa acaaaaacaa
     9361 taacaaaatc atctcttggg ttccggttct tacaactaaa attaagggct aaaatccaat
     9421 cccggtcgga aacctggctt caggccgctt aaatgccttt gaaggatgtt ttgatcggcc
     9481 cgggtcctta ccctggaaag ggcttcaagg aaatgttttt ccgcgatccc gtcatccagg
     9541 cctgtataca atttacccag caaagtatgg taatagggat tctccaggag tttgagcttt
     9601 tccgcttctt ttatcgccgc ttctttgccc ttgattttgg acaaggcaaa aacccggttg
     9661 agggcggcaa tgggtgaagc ttccagttgc aaaagccggt catagagatt caggatatgc
     9721 tcccattttt cacggctctc tttttttacg gtatgccaat aagcgatggc tgcttccaaa
     9781 tggtatttgc tgaccacttc gccccgggaa gccttgtgca ggcattcaac acccctggaa
     9841 atcagggcct ggtcccagag ggtttcatcc tgatcctcat acaaaaccat ttctccggag
     9901 gctcccttcc gcgctttaaa ccgcgaagca tgaaaacaca tgagggcgag cagggcatgg
     9961 acggctgggc ggtccgtatg ccggtcttcg gtcagcaggg agaccaggcg catagcttcg
    10021 agacataatt cttccctcat gaccgaatcg tggatctctg aataataccc ctcgctgaac
    10081 aaaagataaa gggtggcgag gacggagccc aggcgctcat tgatttcatg ttcgggcgga
    10141 aaacggagag cctctttctc cgcgcacaat ttttcctttg cccggtacag gcgtttgtgg
    10201 atcgtttcct tgctggtcag aaaagccctg gcgatctcgt caatcccgag gccgcacagg
    10261 atccgcaggg ataaggcaat ctgggcttcg accggcaggg agggcttgca cagggcaaaa
    10321 agcatttgga gctggctgtc ccggatattc tggtcggaga ggtcaatgtc cgggagaaag
    10381 cggtgatctg cattcctgat ctgggcgggc aggattttag tggaatacaa tttgtttcgc
    10441 tgaatataat tgaacgcttt gtttttggcc gttgcataca accaggccga ggggtggtcc
    10501 ggtatccctc gataaggcca ggtctgaagg gccgccagga aagtatcagc ggctatgtct
    10561 tcagccgctt cgatatggtc cataccgaaa gcgttgcaaa gcacagccgt gatcttcctg
    10621 aattcggtcc gaaagagatg ggggatcagt tcctggtgat ccatctggta aaattatccg
    10681 gaaaaggcgc ttttaatgta ttccatccct ccgggcaatt tttctgacct ccaccgtatt
    10741 gccctcgccc tgcagcaccg ggctgccttt ggcaaagtcc accgcttctt cggccgattc
    10801 ggccctgacc ataatatagc ccccgatggt ttccttgata tccccaaacg ggccattggt
    10861 caccatattg ttgtacccga ccacgcgggc atcctcaaag agcaggccgt tgccgcccac
    10921 aaactttcct ttagcggtga tgccgctgat ccagtccatg gtctgtttca tccagatttc
    10981 catttgttcc ggtgaagcca gtttctggcc atcctggtgc ctgaagatta acataaactc
    11041 gtccattttt taaaaatttt aggtgaatga atatcgcaat tacctgtata agacaatcgg
    11101 gttggcgtaa atgggacatt caggagcagc ccggaattta atcagacgga ggatataaac
    11161 catagattca atggacggta tagggttaat ggcctcctga ttaaggaacc cgacggcgtc
    11221 tggttcgtcc cttggtccac ttggtgtgtc ctagataact aaatgccaaa caattggtaa
    11281 tcaataattt aatttctaat caccacaaag gcttcttatg cccgattttt accaaagtta
    11341 tctttcctga cgttgcagtt cacccaactg ctgcacactc cgtgggcaac acatagtgtc
    11401 ctggtgggaa acctcttaaa gcatcgaaga tgctccacgt ttttaatact cattcaaagc
    11461 aaaaccccgg cttcggagaa gcccgacatc ttcgaacata aatatttaaa ggtcttatcg
    11521 gaaaccccac ccaaacgatg gccaccgccc tcatctttat cgaaaaaaag aatgaaaatt
    11581 tttttattgg gactgcaata gttgggcttc tccgaagcca ttgttttgtt cttatttttt
    11641 accaataagg tcatgcatct ccgatgccgg aataaacagt aggaattaat ccttgctcca
    11701 atatgcgcct tgccgtgggc tatcgctggg ctacccgagg gccacatcca gaaacatcat
    11761 ggccgcaaag cccagcatgg ccccgatcgt ggaataatcg gtttcttctc cggtttgtga
    11821 ttcgggaatg atctgttcga tgactacaaa gatcatggcc ccagcggcaa atgcaagggc
    11881 atagggcaat accggcgcta cctggcttac caaccaggcg cccagtacgc ccgccacagg
    11941 ctccaccatt ccggacatct ggccgtaaaa aaatgccttg cgccttgaaa acccttcccg
    12001 tctcagggga atgctcactg ccgccccttc cggaaaattc tgcaagccta ttccaatcgc
    12061 cagggtaagg gctccggtca gggcttccgg cgaagtatag ctgtataaag cgccaaaagc
    12121 cactcccaca gccagccctt cgggaatatt gtgaagggtg atggcggcaa tcaacaatac
    12181 gctgcgctgc caggatgttt tcaccccttc cgccttgtcc atggtccggc tccagtgtac
    12241 atggggaagg gttttgtcca tgaccagtaa aaaagctccg cccgataaaa aaccaataat
    12301 ggccgggatc caggaaacct gtccgcgcga ttcggctatt tctatggcag gtttcaacaa
    12361 agaccaaaaa ctggcggcga tcatcacccc ggctgaaaaa cccagcatgg aattcaacaa
    12421 ttttctgtga gccgttttaa aaaagaatac catggccgag ccggaagccg tcaccagcca
    12481 ggtaaaaagg gtggccagca aagcaagcca aaggggatcg tatttcaaaa gcctttcaaa
    12541 atccatgcgg agaaataaag ataaaaagcc ggcccgaagt acagacctcg cactcctcaa
    12601 tcctcaatcc tcaatcctca atcctcaatc ctatctttac cccatgaaac gacgtgactt
    12661 tttcaaaacc gcttccccca tggttcttct tttggcaaat gggcagatta cggaagcggg
    12721 cagacttggt ctggaatcca tggaccggcg aaagcccatc cttcgttttg cagtggcttc
    12781 agatggccac tatgggcagc cggatacccc ttaccaggaa tatttcacca ccctggtcaa
    12841 ccggatcaat gaggaacatg cccggcggaa attcgctttt gcagtgatca acggtgatat
    12901 catccatgat gacaagtcct tctgtccggc agccaaggcg gccctggacc gtttgcatct
    12961 taaatattat gtgagccagg gcaaccacga tatgctcacc ccggaggaat ggcaagccaa
    13021 ctggcagatg cccataaatc tggactttaa gatcaaaaag tccagtttcc tcatcgccag
    13081 tacctccaat gcccagggca cctatctctg ccccgacctg gcttggatga aagaccggtt
    13141 ggaagcccat cgccgccagg accgggtttt tgttttcatt cacatcaatc ctgccaagct
    13201 cacccgtcat gccgtggatt gcccgcaact ctacgaattg tttgaccgct accccaatgt
    13261 caaagccgta ttcaacggcc acgaccacga tgaggagggg atcaaaatga agggaaaaat
    13321 tccttttgtt ttcgatgctc atttcggcgg atcctggggc acggaatacc ggggtttccg
    13381 ggtggtggaa gtgctccggg acgggaccat cgccacctat atcctgaatc ccgtggaccg
    13441 gatccaggaa ggggtactct gagagaaacc atcttttttg taagtacgca aagtaaacca
    13501 agactggatt tatttgttta aagcctgact ttgaacatct ttttcatttc atggttaatt
    13561 acctggtagt cggtgcctcc tccggaatag gacaacatct ggctcatgat ctcgcggaag
    13621 ccggccaccg ggtctatgga acctggttca gccatcccgt ggtgacgttc ggccccttca
    13681 ttcagtatca ccctttgaat gtcctggatg acacggtctc tctggatttt ctccccgaat
    13741 ccctgaatgg cctggtctat tgtcctggga gcatcaaact gaggcctttt gaaaaactga
    13801 agacggcaga ttttatttcg gattaccagt tgcaaacgat cggtgcggta aaaatcatcc
    13861 aggcggcctt gccgaaactg aaacaatccg gaaatgcatc ggtcgtgctg ttttccaccc
    13921 tggctgtaca gttaggattg aattttcatt ccctggtagc ttcttccaag ggtgcggtcg
    13981 aagggctggt ccggtcactg gctgctgaat atgccccaaa gatccggttc aacgccatag
    14041 ccccttccct cacagacacc ccgctggcaa gcccttttct ggataccgaa cacaagcggg
    14101 aaataaatgc tcaaaaacat cccttgaaaa gaataggaaa taccgcagat atggcaaaca
    14161 tggccacttt cctgctttca gaaaaagcca cctggatcac cggtcaggtt tttcatgtag
    14221 acggaggact ttcgacaatc cggacctgat ttatgaagat cctactgacc ggggtcaccg
    14281 gatatattgc acaccgactg cttccggtcc tgttgcagca gggccaccag gttatttgtt
    14341 gcgtaaggga taaaaaccgt tttgttgcgg atgggtccca aaacctgggc gtcgtggaag
    14401 ttgatttttt ggataaaaag accctgaacc gcatcccgga ggatgtggat gccgcctttt
    14461 acctgatcca ctccatgtcg gccggatcag gggattttga atcgatggaa atcgcttgcg
    14521 cccggaattt cagggaaagg ctcgaagaga ccggggtgaa gcaggtggtt tatctgagcg
    14581 ggatcgtcaa tgaaacggaa ctgtccaaac acctggcttc aagaaaaaaa gtggaagaca
    14641 tcctttccgg agcccgtttt gcactcacta cactgagagc gggtatcatt gtgggttccg
    14701 gcagcgcctc ttttgaaatc atccgcgacc tggtcgaaaa attaccggtc atggtggccc
    14761 cccgctggct caatacccga tgccagccca tcgccatcag aaatgtactt gaattcctga
    14821 gtggggtcat aggcaaacct caaacctaca ataagagtta tgatattggc ggcccggata
    14881 tcctgagcta taaggaaatg ttgcttcggt ttgccaaaat aagaggcctg aaaaggcgaa
    14941 tctgggtgat cccggtgctt tccccccgga tctcctctta ctggttgtat tttgtgaccg
    15001 ccacttcatt tgctcttgcc aaaaacctgg tcgacagcat gaaggtggaa gtgatatgca
    15061 aagaaaatga cctggcctcc ctattgggaa tccaatggat cgactacgat acttccatcc
    15121 ggctggccct ggacaagatt gaacaaaacc aggtcttgtc cagttggaaa gatgcccaga
    15181 ccagcgaact ccttaacaaa ggcatttcac atctcatcga ggtccctgta catggatgtt
    15241 tcatagaccg gcgcaaaatc aagatcaacg acgtccggca taccctggat aaaatctggt
    15301 ctattggagg aagaagaggc tggtattttg gcgactggct ttggcaaatc cgcggtattg
    15361 ttgatcaatt ggttggcggg gtcggcatgc gcaggggacg gaaaagcgag accgggatat
    15421 cagccgggga cgcgctggat ttctggcgtg ttttatatgc cgataaggaa gaaaaaagat
    15481 tgctgctcta cgcggaaatg aaattgccgg gcgaagcctg gcttgaattt aaaattgacc
    15541 gggaaaatat actgcgtctc acggcgacgt tcaggcccct gggagtctgg ggaaggctgt
    15601 actggtatgc ggtactgcct tttcatggtt atatttttaa agggatgatc cggaaacttg
    15661 cagcggttta attacccttt ggcggtcata gcgcctcatt tcgcataagg gttctggcaa
    15721 ccatgacgta gatttgaggg cattggcagg ttatccagcc tgccacccat tcattgagca
    15781 tgaaaaagtt agccaggctt aaaacgggct tgagttcaga acaggtcgtt gcgagccgca
    15841 gtgcgcacgg cagcaaccgc ctccagctcc aggaagaccg ggtattctgg catgtcctgc
    15901 gatccgtggt cctggaaccc atgtttgtcc tgctcgcggc agcctgctcg gtttatttca
    15961 ttcttgcgga atatacggaa ggcgtgatca tgctggtggc catccttttt gtctcgggca
    16021 tttctttctt ccaggaatac cgcagccggc gggccatccg ggccctgaag gaattatctg
    16081 ccgggcaagc caaggtactc cgcaacgggg ttgaaatcaa agtggatttg gaagaaatcg
    16141 tggtggggga cgtcgtcctt ttgcaggaag gtgaaatcgt gcctgcggat gcccggatcc
    16201 tggcttccaa tgatttttcc cttaatgaat ccatcctgac aggagaggct tttgccgtat
    16261 ccaaaaacgc ctacgaccaa aaccaggttt tccggggcac cctggtcacc agcggcagcg
    16321 cctggatcga ggtcacggag gtgggcctca acacccaatt cgggaagatc gggctttccc
    16381 tcaaggaaat ttccgtgatc cagactcccc tccaggttca gatccggcat tttgttcgtt
    16441 ccatggtctg gatcggatcg gcggcttttg tgatcgtagt cgtattcaat tatctgcaat
    16501 ccggcgatat cactcatgct tttttgcagg gtttgaccct ggcgatgagc atccttccgg
    16561 aagaaatccc ggtggctttc agcacttttc aggccctggg ggctttccgc ctcttaaagc
    16621 aaaaaatcgt tgtcaaacaa ccccaatacg tggaaaccct gggctctgct acggtgattg
    16681 ccgttgataa aacgggcacc ctgacccaga ataaaatgga gtttggatac ctctatgatg
    16741 ccccctccgg cctttccatc cggtgcgatg aaaaaagccc tttgccgctc cggctgatcg
    16801 aatatgctat gtgggccagc gaaacccagc cctttgaccc catggaaatc accatccacg
    16861 actggtacgg ccgcaaggcc ccggtggatc tccggtcaca gcacagccag gtccatgaat
    16921 atcccatcgg gggcatacct cccatcatga cccatgtctt ccaaaaccat aaaggagaaa
    16981 ccattattgc cgcaaaggga gcgcccgaag gcatcttgcg acaaagcagg atgacgatga
    17041 atgaactgtt gaaagcagaa caacaggccc tggattatgc atccatgggt taccgcgtgc
    17101 tgggggtggg caaaggaagc tgggaaggaa aagtatggcc ggtttcccag gagtcgttca
    17161 agtttgagtt tttgggcctc atcgctttcc aggaccctcc caagccctcc atttcccaaa
    17221 cgctcaaggc ctttgaggat gccgggatcc aggtcaaaat gatcaccggg gactatgccg
    17281 aaaccgcttt ggccatagca agccaggtcc atttcaggca caacgggaaa atactcagcg
    17341 gcaacgaaat catggatctc cgtgaagaag aactgaagga aaaagtaaag gatgtaaatc
    17401 tttatgccag gatgtttccc gaggcgaaac tgaaggtgat cgaggcgctg aaagccggtg
    17461 gcgaagtggt ggccatgacc ggcgacgggg tcaatgacgg gcccgccctg aaggcggccc
    17521 atatcggtat agcgatggga aaacggggca gcgaggtggc caaaaacgcc gcttccctga
    17581 tcctcacgga tgatgacctg gcccatatga cggatgcggt ggcgctgggc aggagaattt
    17641 tcagcaacct ggaaaaggcc atccggtata tcgtctccat ccacatcccg atcatcctga
    17701 tcgtcaccct gccgctcatc ctgtcctggg cattcaccga cctgttctca cccgtgcacg
    17761 tcattttcct tgaattgctg atgggaccca cctgctccat catctatgaa aacgaaccca
    17821 tggaaccagg cacgatgcaa cgcccccccg tgagaacagg ccgggttttt ttatccttcc
    17881 ggcaccttgc actcagcatc ttccaggggc tggccatcac cgcgggctgc ctggggatcg
    17941 gatactatta tatgcaggca ggccatgatg agacgaccgt ccgcacggtc atattcatca
    18001 cccttttatt cagcaatatt ttccttaccc tggtcaaccg ctctttttat tattcggttt
    18061 tcagaaccct gctttataaa aattcattgg ttcccctcat cctgttcatt accttattgt
    18121 ttatcctggc cttcctgtat atgcctttca tgcaacaatg gttccggttg cataccctgt
    18181 ccttaaacag tgttgggcag tgcatcgctg tggccctggt ttccgtattg tggatagagc
    18241 tctggaaatg gggtaaaagg aaggggtgat atgccaaatt ttaaaaattt ggcatatctg
    18301 catctaaaat atgagattat tcaaaggggt cctgaggccc tgaatacaac cagcgcaatg
    18361 aagtatgatg ctgtttcgtc ctttcaaatg gtcaattatt tctaacactt tattttccag
    18421 ttggataccc ttcttctgaa ttcgcgtgac gccttccaca gcataggcct gcaattcaaa
    18481 atttggtgat ttaatccatt caacaagctt ccggatattc cccgtttcaa cccaccttaa
    18541 catttgtttt gcctggagtg gcaaatcatt gccggtttcc ccgcatccca tggagtaata
    18601 ataatcttgt gcaagcactt tattaaattc atcaaatcga tagttcgtat cgtaaaatga
    18661 attgtattga accatcaatt cctgaatccg ctcgtgatgg gttggaaaat atccctggac
    18721 tgcccagaat cgatattttt cccttttggc caattcaccg tacacgatcg aatcctcttt
    18781 taaaattagg gttaaccgaa aatcccaaaa ggtattcccc tcaatgagtt cacattccca
    18841 gaccacatag gaaaacccaa aatccatcgg cctgttcaaa aaaggctcag gcggtgcata
    18901 aatgtatttg gaatggacag atttaattcc ttccagattt ttactggcca ataattcctg
    18961 aacaaacttc tcccgggttg atgacctggc gtcgttatag agccatatag ccaaaatgac
    19021 aagaagatgc ttcataaggc caataattca atatgcatag gttcttaacc gcacctggat
    19081 ttaaaattaa agttatttta gttcatgaaa aaaatattgc ctgcctccct gttcctgacc
    19141 ctggtcatga atgcctggtc ccagccctcc cctccccttc gtgtagccat cgacggcatg
    19201 gtccacgacc atgtcaactg gatcctgggc cgaaaacccc tgggagatat tgagatcgtt
    19261 gggatcgcgg agcccaacca acaattggtg gacgcttatg tcaaaaggta tggctttgac
    19321 aaaaagttgg tctacccttc cctggaaaaa ctggtggaag cggtgcggcc cgaagcggta
    19381 ctcgccttca actcggtcta cgaccacctc aagaccgttg aattttgcgc accgaggggt
    19441 atcccggtca tggtggaaaa accccttgcc gccaatcttg aacaagccaa aaccatgctc
    19501 accctggcga aaaaacacca tattccttta ttgaccaatt acgagaccac ctggtatggc
    19561 agcaacgaaa tgacctatga cctggtccac acccaaaata agatcggaga aatccgcaag
    19621 ctcgtttttc acaccgggca ccaggggccg gtggaaatcg gctgcaagcc ggaattcctg
    19681 gcctggctga ccgatcccgt gctgaacggc ggcggcgccc tcatggactt cggttgttac
    19741 ggggccaacc tcagcacctg gctgtttaaa ggaaaaacac cggaatcggt cacggcggtt
    19801 gctctgcaga tcaaacccaa tatctatccc aaggttgaag acgaggctac catcatactg
    19861 aaatacgatc aggcccaggt catcatccag gcttcgtgga actggccctt tggccgcaaa
    19921 gacatggaag tctatggaca aaccggatat atcttttgcc aggacaaccg tcatttaagc
    19981 ctgagagaag gtgaaaaaaa agaaaccttg cccttcaccg ctgccgaact gccatcctcc
    20041 cgcaaggacc ctttcatcta tttcaaaaac gtgatccgcg gagatataaa attatcgccg
    20101 tccgaccttt ccgcaccgga aaacaatgaa acagtgatga agatcctgga agcggcgaaa
    20161 agatcggcta aaagcgggat gaccatcagg tggagtgaac tgtgagaaat cagctgtgag
    20221 ctatcataaa tcagctatca gctatgtgct atgagcgatc agctatgagt gcattgaata
    20281 gtacaggtat gacgaataaa agtgacccat tacaaaatgc cggcagcgcg ttgggagaag
    20341 ttattaagat ttgtttacac tcacgtttga gcttacaata aggtaaccat ccacccagcc
    20401 attaagttta aataagaatt accattcgta acctgtattg tcggcctcag gtaaaagtta
    20461 ccaagtttaa aggccagttg gtaattgtaa taaggaaagc tatatgattc ttcccggatt
    20521 ccaagaggtg aggggtcatg tctgtaatga gaatggcccc ggcccaaacc aaaagatggg
    20581 gaaatcgaga acagattggc aaaatgaaaa ttcctgtaaa cgctcaggcc atacaaaaag
    20641 gaatggatac tggcattgtc tccggggctt ccatagatga gccggttaaa ttggtatccg
    20701 gcatcaatgg aaaaagatag tatcgtatgg tgttgctctt gttttagcac caggtaatta
    20761 aatcctaaag caccgagata agtgtttaat tttacgttgt tgttttgaac ggtatactta
    20821 gttatttctc gaccaatggt caggctgccc gtaaatcttc ccctgaaggt atatcctcct
    20881 gtcagtgccc agaggtttga acggccatcc ccattattca gatgagaata cgatggggcc
    20941 aggtggtatc cactggtctt taccggaaac aaataattct gagcagacaa cagaaatgtg
    21001 aagtgaacta caataaatgt tagggaaaag cggtgtttca tttaaaaaat attttgttcg
    21061 tttcaatttt tctttatcta tggatttttc agtcactttt atttgaccgg aagcccttac
    21121 cctttgaccc ctatgaccct tgacccctct tgacccttga ctcttgactg ttgactattg
    21181 cctattggcc cttcccctcc tattcacact tcttctcctt attcccctta gtccggtcca
    21241 ccttcccgtc cggacctgcc tgctcaggga aataacaacc gcttttttgt ttgatttcat
    21301 ctgcatcggc tccgcattct atccggcagg ctcttcctgt taaaaccctg gtatcacctt
    21361 gcctattcac cataattaaa gaatatttaa aagctccgat gtacggcagg ccatctttcc
    21421 tcataccatc ccaaccataa ttttcaaggt ttgaatagac aacggttggt ctataaaaaa
    21481 ggactgtatc accaacatct gtaaacacag taaaatcaat gatctctacc actttaccac
    21541 taataaatgg ataaaacaaa tcatttatac catccttatt tggcgtaaat acattgggta
    21601 catagataga catatcacta tgaagatact ctatgggctc agccccacag caagcagcat
    21661 atttatcagg cttttgagct tgggtctgat tgaaaacaat tacgaaaatt actaaattta
    21721 aatatttcat aacttttgat attaatacta aaaaactatt gtttaattac gctgactatt
    21781 cttacaatat ttccatcaga aattcttaaa taatatctac ctgctactaa acttgaaata
    21841 tcaattttac catcctttaa tgtccctgag taaaattttc tgccaagaat atctgtaata
    21901 aagatttggc tgttagaaga taagtaatca gatctgactt ctatgaggtc attagttgga
    21961 ttagggaaaa tattgatata atgatcggct aattgggaat gagtaacctg ataattatta
    22021 tttgcaatag gctcaataaa gcttcttata gcttgtggac aaatattccg ggcagttcga
    22081 tcccctgctg cgtcgtaagc gaaagtaatg cactgagatg aagctctaag atgtaagcca
    22141 attatcgcga atattaaaat atgtttcata aataaattta aatttaatac gaattaagcc
    22201 aaagcaatat tgaaatgact tcatttataa agtcttttaa aaaatagttt ttattcttca
    22261 tctaccatat tatttttcat aggcaatgca atttcactac tattaaaatg gtttttttat
    22321 atttctaaat atttttattt tttttagaac tgttcaattt atttatagcc attttatatc
    22381 ttattttata ggatggagct aattttcgtt tatctatttg gtcaataaaa tttacaaaat
    22441 ctttgtaatt tctttcttcc caagcttttg tagcagcatc taatttttga ctaaccaata
    22501 gttgcatcgt gtatatttct ccttctttgt aaacatattt ttccagcgca cttaaagcat
    22561 atttattacc tgcaatcaat ggtttccctc ttccattaaa aaatgtacaa agattcttgg
    22621 caaacgtatc tggtgttaca ttattgattt ttagatctga gttaaaagca tttttaagca
    22681 catactcatc aacagcatat aataaatcat ctttctttcc aacataaaga ttctgagatt
    22741 tctctttctc attgtaatta aatagaatta ctactttatc tgatttcaat ttcatatgat
    22801 ttatagattg ctctatgatg atcagatggt gtttctgaaa aatcaaacct agcttttcgg
    22861 atataagttc attaaagtcc ataactttta tcttattggt ggtatccatt ctccgctact
    22921 tggactatat atccaaccgc gagtatgtaa aatgtatctc tcagcattta agaatccgcc
    22981 ggtaggaata agatttccat taggcagtct atatgaatca aaaattggtt tacctaaatt
    23041 catttcgcta cgaagggcgc tataattggc tttccagttt aattttgcca aacccttatt
    23101 tggaagatga agcatataat cccctatttt ccagcctgta gatttacttg tttgctgggc
    23161 accccactta aataaattta atcctccttg ctgtgttcca gtcttggtgg caaatcttgc
    23221 agcattataa attagcccgt ccgccccaat tatcactttc tcagcaccaa taacaagctc
    23281 agggacgtta tattttataa tatcatcagc agtttctcct attatccttg tagttcctgc
    23341 acctgttgta tagcctccaa atgatctcca agaatagtta aaaatgctgg agccaagtag
    23401 atctgccatt ttaccggcac ctgaagctcc aaaggcgtct aacgcaacat gaaaagcata
    23461 ttttgttata tctttaacat tatctaattg aggcaaatgc ccatatgata tgtccaatag
    23521 tacattcgta gtagctgtgt atgaaccgcc tgctaatgga ttgaccacgc taatgccccc
    23581 acctaaaaac cctgacataa aatagcttaa accctgtccg aaattttgga ctttactaaa
    23641 attactccat aaattaagac ctccacctat tagtgctgct ccggcaaaaa ctaatggtat
    23701 aatttcacct gagggatcgt tatatttcaa tgggttattt aaaacataac tatacctatt
    23761 aaaggcctga ctaaattctg gaaattggac ataattatca acagccaaca tccttcccac
    23821 aatcggatca tataatctgc cattcatatt tattaacttg aatagctcca gatgttcatg
    23881 tccagtaaat cccctggtca accagatagg tggtgcagtt actccgctat aagtccagtt
    23941 ggagtagtta cgatatctac cccaggcatc aaagttttga ttatggacta cagttcctat
    24001 attattggta acatttaaga ttgagcctaa atgatctgta taagtgtaat agtatgtatc
    24061 ggtcccgctt tctcttatga ctattgccat caaaccgtca cccgcattaa tgtaatggat
    24121 atattttgta ctacccccgg ttatatcctt ttcataatct ccaaaaaagt aacgggtatt
    24181 ggtggtggat ccgttttgga tcaaaacact ttttattcta ttttcatcat atccataagt
    24241 ataattcagt gtattaattc cttctgtgag ggaagttggc tgtaagaagg aagaataagt
    24301 tggaagggac tgactggcgg aagatatggt tcctgcagaa ggattgattt gagtgacggc
    24361 atttattctt gaacttgtat atgcatatgt acccgcatca gtcttactgg aaatgtttcc
    24421 gttatttgaa tagacggttg tataagttgt taatcctgtt accagactgc tcgtcagcct
    24481 attcaggtta tcataaccaa aagtttctgt tttgctggcg ataccatcta ccctgcttgt
    24541 taaatttcca ctattgtgat tccagttaag acttaagttt tgaacacccg tagtactata
    24601 gttggttgga atcccataaa aataagtatt cgtacttgat ttgccattcc ccaggctata
    24661 tgttttatat tgatcataac cattcatggt tccatttgta aaaatggtaa cagaattatc
    24721 agtattctta actgtactaa ggtatccatt tgcatcataa ttataattga gcgcaaagga
    24781 tgaaggatag gaaactgtac ttacattact ataactagca tcgtaagtat atgtcttagt
    24841 gtgtgcaact ccgtccacgg tctgtttatc tgtccataaa tcaccatatc cattatatgt
    24901 atactcctgc agatcccctg cgaagccggt aatctttttt attttgccaa ttgaggcata
    24961 acttcctgcc tgatagtact cataagtagt tgttccttcc ggcatagttt tggtgagaat
    25021 tctacccatg gtatcatagg tcatagtgtg cgtctggctc tttgcattta cttgtgtaat
    25081 taattgaccc aaagcatcgt aatcatatga ggtcgtacca gcattgatat ctaccatact
    25141 ggattgcctg gcatagacat cataattatt agtaactaaa gtcgatgcac cattgattac
    25201 agttttaaga cctccatgac tgtaataggc gtaattgagt gttcctccat aatcggtggc
    25261 actgattgtt ttcccggttg cgtccgatac tttagaagat acctgacttg caggattggt
    25321 ggaagtggtt gtaagattcc cacttgcata aacatatcct aatgtgctgg tatagttagt
    25381 tcctccttta ttttccacaa tagaggcgag ccttcgatac ccatcgtaat tgaatgtagt
    25441 caataaacct gtttctcctg atttgtaggg tcctgtggtg gtgaatactt ccccattagc
    25501 tgtataggtc ttctcagtgg ttatccattg gttttgccat ccttctgtct ccgtcctccg
    25561 ttctctatcc aatacatcgt tgtaaatttt aatatcaggc ctatgtgggt gagtaatttg
    25621 tttataatat cttgacccat aactcgtggt aaaattatat gaagtagtta tgttataaaa
    25681 ttggggagtg gtcatagata tctccctgcc aaaagcatca aaagaaaatg aagtaattaa
    25741 gcccattatg tcagtcacac tataaggctt accccataag ctatgatagt aggtgtaagt
    25801 aactcctaca ggagtggtgg tagtattggg ccatctgccc attgtatcaa actgtgatga
    25861 gaagatattt ctagcagtaa tacctgatgc gctaatacga gcggatattt gatttcccaa
    25921 attattataa taataagtag tggtgacagc tttgggttgg ccaacaaact caacctttgt
    25981 tagcatctga ccaagactat tgtaagtcat atttatgaca gagctatatg ctgtttgccc
    26041 ggtacgggta tttgacaccg ttactgaggt tggtagtgca ggtaccggag tgccgtattg
    26101 cccataagtt gtagtagtgg ttgtagtttc aacactatta ttgttaacta cttgtgaggt
    26161 tacatttcca tatgaatcaa atacagaatt tgctgttgag agagttcttc cttcaaaaac
    26221 tttattctca agcacattat taatgcgata ccaatatcga ccagtgcctt gagataaaaa
    26281 ttgattagaa ttggtaactt ccttgactag tgagccattt gaaattaata taatactatc
    26341 cttatacggt gcagctacat aataagtcaa attaaattcg ttctctgact tttttctgaa
    26401 tcccgaggct gcattgtcgc tatttatttt tttgaatccc aaaaatccct tcccttcttt
    26461 atgtaatttt gcttctgcat aattatatgt catggagttc ataccgccga ttccattatc
    26521 catctttatt tcagatacaa aatgcatggg taattgtata ttatttacat tcccggagag
    26581 tgtagggcct ttattataaa aggttcccga ttcattcatt cttttataaa tccattgggt
    26641 ggtatagtcg taaccatttt tgactttttc aagaaaaagc tccttgccaa atggcataaa
    26701 atgataaata tcaaagtagg acagataatt agttcgaact aatatttcag atttgccatc
    26761 tccattatag tcaaatggcg taagcggcat ggtatttaaa gtagtagtag ttccagtgta
    26821 gaggtaaaaa tagaaactac ttcctcgaga aaaatataca tgatacgttg aagaagtggt
    26881 tccaagaatg ccatgaaaaa tatcagtttt gccatcaccg ttacagtcca tcaacagtat
    26941 tttatctccg gaatattcag atgtaactgt gggtactgta gtaaaagtaa aagcactttg
    27001 gtcaaatgaa gtaccggttg ataggcattt ataccatggg gcattattta gagtagtact
    27061 actcctatat aaaatatcca tcttcctgtc cccattaaaa tctccaaaaa atggaagatg
    27121 atcctgggta ggaaatcctg cataatatat gctacgagcc acatatccat caatagtata
    27181 tatctctgaa ttgttaccgc tcgtaaccat tagatcactt ttaccatcgc cattaaaatc
    27241 caatacctga atccgcttgg ccgattgcca gttattgact ggaatattaa tcgtaccagt
    27301 cgttcctaca gtaagaaagg atggcgaact tcctccatag taaatatgta tgccgattgt
    27361 agtaggggaa gaagtatatc ctaaaatact caataaatcc cctttgccat ctccattaaa
    27421 atccccagca aacaaaaagt gtcctgttgt agagttgatt ttactatacc cggtataggc
    27481 attgatagtg acagagtcac taaaaggggt agtattggat ccattataaa ttctggcaaa
    27541 ctgaaaagcc catgttccac cgtatcctgt tgttttagca gccgcaaaat cgtctatccc
    27601 atctccggaa aagtcattag tcaggaaagt atataagtca ggtattgatt ttttctttaa
    27661 agctgaaaaa tttccagcta agtttttaac aattggggca ctaaatgagg tagcttgatt
    27721 aatagcagga attgctttaa ataatcttat ttgggagcaa tattcaatat tatcggataa
    27781 tattttgttg gcttccacta aatctgaaaa accatctcca tcgaaatcac ctgagtagta
    27841 attgccatcc tgaatacctg tagtattagt tccgtatcgt tgaacaatgg tcggttgatc
    27901 accatatttg aagatcgtac tatttaattg actgccatta ctacctttct cgataatttc
    27961 attaagatag ctattgatat tatcccatcc atagttaaat tggtaaattt taaatgctgc
    28021 actactttca ccggttatag taatattatc caaaaatttg tcctgtgttc gtgcagtacc
    28081 agcttcatac actgtgttaa catctgacct ggagacataa ttgaatttga ttttattata
    28141 aggagtgaga cctgctgctg tgttacctgt ataattgatt tcattaaacg ttatagaaac
    28201 accgactcca ttgttatacg aatattctat atagttcccg tcgggataag tgatcttaga
    28261 gagcaaccag ctcatgacct gggtttgggc agtattcatt gctcgtgagt ttgaggttcc
    28321 accatattcc atggttctgc catctttcag aaatacagta aggtattgag gcccggtacc
    28381 tattgaacca aatgcagtaa ttgttgcaaa attttcagat tccgtaccat atgtagaacc
    28441 agcagcgcca taagtcccgg tttttaaaac caatctgctg ccatccaaag aatatctatc
    28501 accagtagtg agctcgacgg gtgccacatt accatcatga taaatcgttt ttggaatcct
    28561 ggaaattgaa gacattccag taatggacca cccctggcct aaaacactat agcctcctaa
    28621 agagttatat agtattgaaa gtgaaggtac tactccatta gtgccggggg gtactactat
    28681 cggaatcgta taggtcgcag caccggataa gtccacatcg gctgaagctg cgactgtacc
    28741 cacaggcagg gccgtattga cagtcttggt aaatgtgccg cttgtgtata aagctccctg
    28801 cccgcttgtt tcaattaaat aaaaaattga aaataacaat cccagaatgt gtgtcttcct
    28861 tttcatttat taacctattt aataataata taatttatcc ttcctaccaa atcacttggc
    28921 tggaatgtgt attttaatcc gtgtttattt catatttgaa ttaaacaccc attgattcat
    28981 tgatcaacaa tgaataaatt cagaatatgg agtttaggaa acttaaattt gagaaaagaa
    29041 agacacagcg ttcaaattca atcgacaggg tattctcaaa agcaagccta agagtaaatt
    29101 tgatggaaac aaatataata tatatgtttt taatttttcc aaatttaata tgttaaatta
    29161 tcaagatatt tgtaatttga taggggtcct gtttaatgtg tatattaatt cagccagagt
    29221 atttttaaaa gataattaca aatagatctg gggagtatgt ctcattcgtg tagaaacaga
    29281 gatcatcggc aaatttgaaa tcggtaattc ttgttttaag tgtgctaatc caggtgtatc
    29341 ttgatttgat tccttgaatt tcgcaagggt gtactcaatg tctcaattcg gggcaggtta
    29401 acaatttgcc ttccgttcta attgctcatc tcgatttgtc catcagtcag aacttttcga
    29461 tctttaacgg tatcaatttt tatcaatgcg tttttttcta ttttttctgt tcggaggatt
    29521 tactcttctt tcagctcaaa ataaaatcat agccctggtc ggcggcaccc tcatcgatgg
    29581 ctttggcggc cggccgatac aaaacagcgt catcctcgtg gaaggcgaac ggatcaaggc
    29641 ggtcggacac cagggcaatc tggccatacc ccccggcgca gaagtcatca gtaccgaagg
    29701 catgagcgtg ctgccgggcc tctgggatat gcatgtgcac ctgatgatca acgggcacag
    29761 cgactatgcc cattgggata aaaaatacct tccccttgca gaagaaatga tcatgcccgc
    29821 ctcggcgcat cagttgctca tggccggggt caccagcgcc cgggacctgg gcgggcccct
    29881 ggaagccagc ctcaaagtcc gggaccggat aaacagcggg gaaatacccg gcccgacgat
    29941 gtatatgtcc ggcccctttc tgcaacacga accctatccc gggactgcag aattccgctg
    30001 gggtatcaaa ggagtggcgg atgccagggc taaggtccag caactggctg gggcgggcgt
    30061 ggattgcatc aaaatgattg accacgacca gatgacgatg gacgaagtga aggccatcgt
    30121 ggacgaagcc cacaaatacc ggctgccggt ggtcgcccac tctcaccgcc ctgaggaaat
    30181 acgccgcgga ttaaaagcgg gagtggattg ttttgaacat accggcctgg cttcggcgcc
    30241 ttcatatccc gaagacatcc tggaaatgct caaagagcgg accgcgcaaa tggccctggg
    30301 ccccatgttc tggaccccta cggtcgaagg actgtacaat tatgaatacc tgcgcgaaaa
    30361 cccggaagcc ctcgacaaca cctcctggca tctgggactg cccgattcca tcatcgagga
    30421 tatccggcaa tccatccgtt accccgggcg gctctcctat tttcagctca accccatccg
    30481 gagacctacc ctgcaaacca aaatcaatca actcaaagag gccggcgtgg tgctcctgat
    30541 cgggaccgac agcggcatac ccatgaaatt ccacagccag agcacctgga atgaacttga
    30601 ggtatgggtc aataaatttg gcctcgatcc catgtacgcg atacgggcgg ccacttactg
    30661 gccttccgta gccatgaaag cggacgccga ctttggcacc atcagcgaag gcaagtatgc
    30721 ggatatcatc gcggtgaaag gggatgtcct ccggcacatc gctttattgc agaacgtgaa
    30781 tatggtgatg aagaagggga ggagggtgaa gtaggtgatt ggtggatggt gggtggtggg
    30841 tggtgacgta tactctgatc aatccagtag cctttgtgtt tgggaggagc tgagctccga
    30901 cctttttggc aaatacctcg taaatgaagt aaatagtagg taggattgag ggttgagggt
    30961 tgaggattga ggagccgaag ccaagctttg tctaaaccac aggggtgttt gcgtgccgga
    31021 aatggcttaa aataatatca tacttcaatc gctacatttc cctttttatg ccctttttca
    31081 acataagcat gcgcttcagc aatttggtcc agggaatagg ttcggtcgag caccggttta
    31141 tatttaccgg cttcgatcag ctctttaata aaaacaatat ctgattttgt gtggcttatt
    31201 actcctgtca aaacctttct gttgctggtc tttgaaatcc ataaaccctg aatcatttcc
    31261 gacatccctg cggcgcttaa aatcattatt ccgtttttag acaatgatct taaactccgt
    31321 gaaatggaca gggtattcac cgaatcaaaa atcacatcgt aggtttcgcc gtttttcgta
    31381 aaatcttctt ttgtataatc aatgacctta tcggctccga tagattttac taatgaaata
    31441 ttggcggtgc tgcaaacgcc cgtgacctct gccccaaacg actttgccaa ctgcaccgct
    31501 gcgcttccca ctgcgccgga agctcctatg acaatcactt tttgtcctgg cttgatcatt
    31561 gcccttttta taaaatgcaa ggcggtaaca ccaccaaaag gaatgaccgc tgcttccagg
    31621 tgggaaatat ttgcaggttt taatgctatg gacgcatttt caggaagaca tttgtattcg
    31681 gcgtaagcac caaaactcat atcggcatga ccaaagacaa ggtcacctgc attaaaatcc
    31741 tttaccgctt ctccgaccct ttcaacttca cctgaaaaaa cacttccaag aatatttatt
    31801 ttcggcctca acaaaccgta taaaaacctg accgcaaagg ggtcggcttt ccgcaatcgc
    31861 caatcccctg aattgactgc ggtagctttt atccttaata aaatttcatt ttgctttgga
    31921 gtgggctttt cgacctcttt aattttaagc acctctggcg gaccatactg ggtgtacaca
    31981 gctgccttca tttctactgg ggttgatttt tgcaaggggc aaaagtagca ctttcgataa
    32041 cttggttaag aaataaaaga ctacctctga ccactgaccg atgaccactg accgatgttc
    32101 gggaggagct tagatccgac cttccggttc gtcagctgaa atgaaataac ttccatacag
    32161 ccgaagccaa gcttcgacta aaccagagga ttgaagatgc aagtttgggt atcactcctc
    32221 attcctctga tgcctttacc catcatttca tcttatccat ttcagaccaa tcccttaaat
    32281 ttactttatg attgaccagg taaagaagat atttaaccga attgcgaatt tgcctgagaa
    32341 gcaacagatc gaactagcca agctcataga ggatgaaatc agttgggagc agacttttgc
    32401 atccagccag gaggcactcg ctgctttagc caaggaggca attcaggaat acaagagcgg
    32461 aaaaaccagt gaacaggact ggtgatgaaa tctgtaacca cctcaagatt cagaaaggca
    32521 tttgaaaaac ttcccaaaag aacccaaatc aaagcccgag acaactaccg cctttggcag
    32581 gaaaatccga atcatcccag cctaaacttc aagcaaatcc attcttctca acccatccac
    32641 tccgtccgtg ttgggatcgg ttaccgggca ttgggcgtta aacaagatca aaccatggtg
    32701 tggttctgga tcggatcaca tgaggaatac aatacaataa ttgattcctt atgaaaatta
    32761 aggatcgatt accccttcta tcaaagaaat atttacgaca cagcctaagt caggccaaac
    32821 aggatagagg tatgaggctg gaggtatgag tggatcgctt acctctcacc tctcacctct
    32881 tacctctaac ctctaacctc taccccggca atttcaccgt aaacgtcgta cccacaccaa
    32941 ttaccgaacg ttttacaaaa atttttccct tgtggtaatt ttcaataatt ctgcgggcca
    33001 gggataggcc caggccccag cctcgttttt tggtggtgta tcccggctcg aagacctgct
    33061 taaactggtt gcccgacaag ccttttcccg tatcggaaag gtctatgcag atataatccc
    33121 cttcttcgta gatctcggcc ttgatggtgc cggtgccgtc catggcatcc agggagttcc
    33181 tcatcaggtt ttccatcacc cagtcaaaca ggggcggatt gatcctcgca aaatgaggat
    33241 ggccgtttaa tgcaggaaaa tcaaagcgga cttttttgga tgcccttttt tccatgtaat
    33301 tccggcattg gtccagttgc tgattgaggt tggtcttttc cagggtaggt ttggagccga
    33361 ttttcgaaaa ccgttcggcg atcagttcca gccgggtcac gtcgctgcgc aattcatcca
    33421 gcagttccat tttctccgga tcctcgctgg acaacagttt caggttttcc acccaggcta
    33481 caatggcccc gatcggggtg cccaactgat gggccgtctc cttggccatg cccacccaga
    33541 cccggttttg ttgttcccgc tggctggcgc tgaacccgat cagcccgatc atcaggaatc
    33601 ccaaaagcaa catcaactgg atatagggaa acaatttgag ccagcgaagc accctcgagt
    33661 tttcaaaata gacattgttc aattcagatt tgatgggctg gatgccctgc tttttgagtt
    33721 tgaccagtct tttggccagg aatgccttgt cttcatcttt cagggggcca tagtttcggc
    33781 cccaggtcac ctggtcgttt tcatcggtgg cgatgatggg cgttttgttg gcttcaatgg
    33841 cctgggattc aaaggtgacg tccttattga tatcgcttcc ggtcttcgcg agggcttcct
    33901 gggctttcac atacagctcc acactgctgc gttccccatc ctccagtttt ttagccagat
    33961 agcggacata gatcatggac aaagccatga ccaggatgcc tgcaatggcc agataaaact
    34021 tccagcggga ttccttactg accagactca tggttttgta gccaacgcta tctattgatt
    34081 aacgcaaaga tcaagagctg tggtttacca aacaaactaa aatttgatta ctggaaatca
    34141 gagtattata aatttagcca ccctttgatc caccaggcca accccgaggt gacggctccg
    34201 gcaagcaggc cccaggccag gtctacccat accatggaac ggggccagcc cgaaatcagg
    34261 gccttattga ccagttcata agtgccgtaa cataagacac ccagaaaggc cccgttttta
    34321 accgccgaca tcaggctgtc cgcgggcatt actgcaaaat acaagagccc gccggtgaac
    34381 agaacataaa acacaacccc tgccgcccag tccggtttgt ccgccaacaa atgccccacc
    34441 tgcctgcgga taaaagccct tgcaaaccca ttgatgaata aaagatcgag gcccgcatag
    34501 atcagcgcgg tgagcaaaaa cagcatggtc gctttcatcc cttaaaatat ttgaaaaaaa
    34561 tatacgtcca atgcaccacc agcaaggcgg caagtccaag gctgaccttc cggtttttta
    34621 atccgctcat cacgcgttta aacccaaacg ccaatatcct tttattggac agcagatcaa
    34681 aaagcaagat accgcccatt acaaggatca aaagcaagag cacggccacc aaaggattat
    34741 aaaagaagga agtcagtcca ttccccccga tcatagcctg cagtgcatgg ctgcccccgc
    34801 atcccgcgca gggaaatcca aactggctga caaaataaca ggattgggcg ggtggggtca
    34861 atgtccggtt cgcaagtaga aatgaaaccg tcaaggccgc cacgcatagc aaggctgtac
    34921 ctccataaat cttccggtcc agcgggccgg tcttcatgtc cgttttgcgc ttacattccg
    34981 atgtttccgg atgtagaacc gccagggcca gcttgcacag gccccggcgc tttccacccc
    35041 gatccgggga cctgcggcga tgagttccgg atcgatttga atctgccggt cttcaatata
    35101 aatcagggat tccgggccca ccaggctgtg gccgctgaag gatttgtcaa aacccatggc
    35161 gacggacagt gcacccggtc ctttagtgat cctttcttcg tttgttttca attgcctccg
    35221 tttcatcatg atttcaaggc cttccagggg ttccagggcc cggatcaaaa ccgcatgggc
    35281 atattcttcc ggcccggtga ccacattaaa caaatggtgc atgccataac aaatatagat
    35341 ataggcgtgg ccccctcttt tgtacatgac ttctgtgcgc ggggtccggc ggttggcata
    35401 ggcatgacag gctttgtcat ccgggccccg gtatgcttcc gcttccacaa tgatgccgga
    35461 ggtcctcaaa ccatcgattt cagaaaccag gaccttgccg atcagggacc ggcccagatg
    35521 caccacatct ttattctgat agaaggtggc cgggagcctt ttgccgctat tgtccatatt
    35581 tgttccaggc tgggatccgg gcagggcttc gggcgccttg catcagcgca tacagttcat
    35641 cccgcatagg cctattggag tccgaaaggt cggtttgttc ccccgggtca ttccggagat
    35701 tatacaattg tcctttattg tctttggaaa accagatcaa tttccaatct cctttgcgga
    35761 tagccctgga ccctccaaaa gacgaatact cccaatagag atagcggtca gggtccatga
    35821 cctgattttt taataaggct tttttaaagg aaagaccatc catttcatct gttttcaatg
    35881 gtagtccaaa gaggtcggcc agggtgggaa accagtccca ggcggccagg atggaaggat
    35941 tgacgctgcc gggttttatt tttcccggcc aggacacaat gcacggtacc cgaatccccc
    36001 cttcatataa atccatttta taaccccgca gcaggccatt gcttttaaaa aaacgggtgt
    36061 cggcgccacc ggtgcccggt ggggtagccc cattgtcact gagaaagagg atgagggtgt
    36121 tttgatcttt tccggctttg gccagggcat ccatcaggat tcccacttcg cggtccagcc
    36181 ttgagatcat cgaggcataa gcagcccgcg gccgatggtg cggaaggtat tggcgctcgc
    36241 ccaggtaagg ggtgtcggca aaatccggat aggcctgtag ttcttccgga ggtacctgga
    36301 tacccaggtg gggcatgggt agggtataat aaaggaaaaa aggccgatcg tcctcagcga
    36361 taaatttcag ggcctcccgg cgcatcagat cgggggcata atccggtccg gtatagtgtt
    36421 ccgccacata atccccggtg aggggcttcc cggacagggg atatttttga tggggggaga
    36481 aatacccatt acgcagggta tcccaccggt cgtttttcca aagatgggaa ggataataat
    36541 tatgggcctg tttctggcag gcataaccat aaaagaagtc aaaacccgcc gcctgggggt
    36601 ccccctcctt ccccggcatg cccagccccc attttccaat gaccgccgtc cggtaagcct
    36661 tggacttcag gaatcgggcc agggtatatt catcggaccg catgggcaat tgtccccttt
    36721 cttcatcgtc cgggaaaccg cccatttcaa aattgtcgcg gatatcggca tgtccggtat
    36781 gtttgccggt gagcaataca cagcgactcg gggcgcagac cgtatttcct gcataatatt
    36841 gggtgaatcg catgccccgg gaagccaggc ggtcaaggtg aggggttttg atcctggact
    36901 ggccatagca acccggttct ccatatccca ggtcatcagc caggatgatg accaggtttg
    36961 gctttgtgcc cggctttaga ccggatagtc cgatgcaggc cgacaacaga aggattgcag
    37021 cttttaagcc atacatattc gcaatatagc cattcccgga atgtaaccaa atatggataa
    37081 cttcgtcttc cgattatgaa gttcaaattc attgtattca ccggcttatg tattttttca
    37141 tgggcaagcg ccccggcgca agaccccatg atcgaacaaa atatcccggg cacttccatg
    37201 caatttaaac tgatcctggc tcccgccgga accgcagaac ttgggcccca aaaaattaaa
    37261 gtgaacctgg atgcctttta tatcggagta tacgaagtaa cctttgatga atttaattgt
    37321 ttccgtgaaa gggatcagga caacgaccaa tcctccaacc cggctattgc ctataaagcc
    37381 gatgccgtga ccaggcccac ctcccagtat gtaaacctga cctttggtat gggcgaccga
    37441 gacggctttc ccatggtaag caccacccag cagggcgcct tgaaatattg ccaatggctg
    37501 taccagaaaa ccggccggtt ttaccgcttg cctaccgaag cggaatggga atatgcctgc
    37561 agggcgggca cctccacccg tttttatttt ggggatgagg agtccaaact ggcagaccat
    37621 gcctggtatt atgaaaactc ggatgataaa taccataaag tagggcagaa acaacccaat
    37681 ccctggggcc tttacgatat gctgggaaat gtggcggagt ggacctccga cgattaccgg
    37741 gaaaactatt actccctggt caccgcagat aacaacaatc cctggatgat cccttcaaaa
    37801 aagcacagcc gcaccgtcaa agggggatcc tatgtggacg acgacccgga ccacctcagt
    37861 ccctcctacc ggctcaaatc cgaagccaaa tggcaggtgc gggatcccca gatccctaaa
    37921 agcaaatggt ggaatacgga ttctccttat gtggggttca ggatcgtaag cccattaaaa
    37981 caacccagcc cttccgaagt ggaggaatat tttaaaaagg ccattgtaga ttgaggctga
    38041 ggctgaggtt aaggctgagg gcttctcaac cttaacctta acctcaacct taacctcaac
    38101 cttaacctca actttttgca ttaatttcgc cttcccggta tatgaattct gaccatcccg
    38161 cctatctcaa aagcgaccgg ctcagcaccc tgagctatgc catccgcggc cctattttcg
    38221 ataaggcgca cgagatgcag ttggccggcc aaaacatcat caacctgaat atcggcaatc
    38281 ccgcaccttt cggtttgttt acccccgatg aaatcgttca ggacatgatc ctcaatatcg
    38341 gggaggccca gggctattcc catcacctgg gcatcttccc ggcccggaaa gccatcatgc
    38401 attataccca gcaaatcggc attccggagg tcaccatcga cgatatctat atcggcaacg
    38461 gggtgagcga attgatcgtc atgaccatgc aggccatgct caacgacggg gatgaaatat
    38521 tgatccccag tcccgattat cccttgtgga ccacggcggt atccctttcc gggggccggc
    38581 cggtccatta ccgttgcgaa gaacaaaacg aatgggtccc ggatgtcgaa gatatccggt
    38641 ccaggataag tcccaagacc aagggcatcg tggtgatcag cccgaataac ccgaccggtg
    38701 cggtctatcc caaatccgta ttgcagcaaa ttgcggacct cgcggccgaa aaaaacctga
    38761 tcatcttgtc agacgaaatc tacgacaaga tcctctatga cggcaataaa cacataccca
    38821 tcgcttccct taacccggac gtcttttacc tgacttttgg cggcctttct aaaaattacc
    38881 gggccaccgg ttaccggagc ggctgggtga tcctcagcgg ggccaaacac cgggcggctt
    38941 cctatattga agggctcacg ctgttggcca gcatgcggtt atgcgccaat gtccctgccc
    39001 aatacggcat tcagaccgcg ctgggaggct accagagcat cgatgacctg gtagcgccca
    39061 aaggcaggtt gtaccggcag ggaaagtttg tacacgatcg ggtcaatgcc attcccgggc
    39121 tttcctgcgc ccttcccaaa ggcgccctct acgtttttcc ttctatagac ctggcacagt
    39181 tccggtttga ggatgataac gattttgtat taaaactcct taccgaacaa aaggttttgc
    39241 tggtacccgg ctcgggattc aatcattacg accgcgccca tttcaggatc gttttcctgg
    39301 cagatacgga tatcctgggc acagccctgg accggatgga ggagctgctt caccaataca
    39361 gggcctgacc ccggcttaac ctcactgggc catcgattcg tatttcgcgg cctggtttac
    39421 atcacccagc tgtcggaggc aattggcaat tttctggtaa aggtcttttt gtttgttccc
    39481 ggcacccatt gcgaatccgt attgctcaag ggccttgcgg taatctcctt ctttctcata
    39541 gatcattcct gcccatttgc tggtattggc caggtcaaat ttttcatccg tcagtttggt
    39601 gaagatcgcc ttggcttcgg tcgtttttcc ggtattaaaa tggatgattc cctgcagcat
    39661 ccgggacggc aaaaattcgg gatgatgttt ggtgctttcg ttgagatagt tctgcgcctg
    39721 gggccagttg ttgctctgag catagatata agccagttga aaccaggccc attcgcttct
    39781 cggattgtgc ctcaggtagg cgttgagttt ttgcaccgcc aggttggcat cattacggtt
    39841 gatggcctgc atggcttcat aatcgtcccg gctgttccgt ttataaatga aacacaacgg
    39901 cttcccgtca atcttgatcg tatgcaggga gtcggctttg ggaaatatat ggttcgccag
    39961 ctggtaagga tgaatgtaca tattcgggaa aatggcatag tcccagtttt gagtcccgcg
    40021 ttcgtaataa cgggtataag tcacggatac gctgtcgtcg aatcccctga aataataggc
    40081 cagttcagag gaaccgttcg tggcgatcac cagccgggta ccggggggat gattgtcccg
    40141 gatatatttt ttaagccact gggctccttg tttgaggctg tttaaggaat aatccaattc
    40201 atactttcca taggcgccgc ccggtcctcc tataaattca ttataataaa ggtattcaaa
    40261 gggatgattg cgcaggatat actgcaaagg atggatcagg cataatacca ggatccctgc
    40321 agtccagagc gaatggcgga tccggggaaa ccgctcaaac aaccattgcc agcccaccgc
    40381 cgaacaaatg gcgataaaag gaaagatgaa caaaaaatgt ctccagcttc catacatatt
    40441 tccaagcttc agataggaat agatcaaagg aaaggcgaag gagaaaaaaa gaagcgccag
    40501 aatattcttt ccggacttaa tcctgttggc caggaaagca aagagggcaa gagccacacc
    40561 cgcgaggact gccacaggca gcgtgatccc aacgtattta atcacgtaat ggtccggcat
    40621 ggaggtggaa agcctgtatt tggattcaaa aatctggtag agggcaaccg ggtggttccc
    40681 ggaactccgg aaaacttcca gggaatgatt gaccggatcc acaagaccat agggccagac
    40741 caaaagattc atcacgaagg cgagcacggt agtcaggata aggccaagca ccggtttcca
    40801 ggtttgtttg tccaggctga tcagccgcgt cagaccattt tgggagatca ggaagagcag
    40861 cgaatacagc cccatcaatg cggtaagtaa aaccccgccg gccaacctgg aagcaaaact
    40921 caaaaacagg gccaagccgg cataaacaag gtatttccgg tggatgaaag gataataatc
    40981 ccagaaccgg atgatggacc aggtgccgaa aatcgtaaag gtggcaaggg ggatatcccg
    41041 gtgattattg aaaacattgc ccatataaat cgggctgagc gtcatcagta aagcggtata
    41101 aaaggcaagg cggtagcccc ccaaacgtaa cccgatcagg gatgcaaaca ggatactgaa
    41161 ccaaccagtc agggcattta aaagatgcct gaaagtaaaa ttccgttcaa gaccaaaggg
    41221 tttaccgatg gtgtacaggg cattgtcaaa gatctgtccg ttgtaatatt ggggatccac
    41281 cccggatctg gttaaggcag aggtatcggc tcctaaagtt ctgtaaaaat gatacacttt
    41341 ctccgcctgg gttagatacc ggttttcatc gctgctgttg ccgacgttta gggcgccgag
    41401 caaggcgtac aagaggctgg cagcgagcaa tccggacaat aggagctgaa agagaacatc
    41461 cctttttaaa taagtggaaa ggccaggttg ccggaagata ctccagggtc cggcaaaggc
    41521 ccgttcccaa aatgcggacc atttttcccg gacaggggat tgattttcct ggggattttc
    41581 ctgattcacc ataaacagct ggtttgccga atcccaatat tgcatcctgt tgcggatcat
    41641 cagcacgagg tccctgccct ttaaatttcc accgtcccgg ttcttttcga aatatccttt
    41701 cgggaaaaaa aggctggccc tcccggggtt gcaactgccc ctctttccgc agtaggccag
    41761 ggcaaccggc cgggagccgg atcccggccc tttgtattcc gtccagcggg aaagctcctc
    41821 ttcccagttc ttatggccca ggtcaataac cagcacattc ttttgaataa aggcggcttc
    41881 cgtttccagg tctggtttta ggggaatcga ttcaaattcc tgatcgaggg ggatagaaag
    41941 gtcggcacgg attaaatctg cattcttaaa cctgtttttt acctggctga ccgcttcacg
    42001 gatttcattg gcttccctgc gccctgcatt gaggagaagg atattgagat tagcggtttg
    42061 gcttggcctg ttctctgctt tactcttttg tttggacata cacgttattc ggatatcagg
    42121 tgttgctgct ttctcaattc ggaggccttg atggagtgcc ggataaggtg tttcaccaca
    42181 ggtttcgctt tgacctggat caggtattca taggcaaaca ggcctttgga aaggcggatc
    42241 aataaaaagt tcagccggac cagggctttt gaccaggcgt tgagcccgaa agcctccgga
    42301 tagggtgctg gcaagccttc cattccgatc accttcatgc ccgattgttc acaaagccgt
    42361 tttatggatt tttttgtgaa cagcctttta tggtgaaggt caagaatgcc cctttttccg
    42421 tagttaaatc cgccaaaaag caattggaac cgggtgataa aaaagccgat gttggccacc
    42481 gtaatataaa tatcggggtc ggtcagccgg atctggtccc tcagttcgtc cagcaattgt
    42541 tcaggctggt ccagcgtttc cagcaggtcc atcaggagga gggccccata ttcgtttttg
    42601 tgcagcgcct ccacgaactc ttgatccaaa aggccctgcg aaccctgatt ccgcagggtt
    42661 gccaatttaa tggtgcaccc tttttgaatc aaggcgtccg caaccagtcc ggaggcgctg
    42721 ccgatgccca ggaccttagt tccggccggg attttttcaa ccacccggct gtgcgcggag
    42781 aggtaaccgg atttgaaccg gtaggggctg tccttttgtt cgaggtcata ctccctttta
    42841 tagaaaatgc cccactgttg gagtctgctg tggatggaag ctttgatgat gttgtacgcg
    42901 taccgggtcc cgttgacata acagatttca tctccgtaat gggtgggaat cgggacttcc
    42961 aggatctgca ttttagccat aaccagctga atgatgatct gggtgtcaaa atggaaatcg
    43021 ttggaattcc gttccaccgg caactggctg agggctttga cggcataggc ccggtagccg
    43081 gaatgaaatt cagacagatg ggttccgagt attttgtttt gaagccgggt caggatgata
    43141 ttcccgataa acttgtaata aggcatttta cctttcaggg catccttttt attgagcatc
    43201 ctggacccga aaacggcgtc tgcctttcct tcggcgatcg ggtgaaccaa gcggtcaaac
    43261 tcctccgggg catactggcc gtcgccgtgc agcaaaagga cgatatcaaa accgtgtatc
    43321 cgcgcataat gatatcccag tttctgattc cccccataac cctggttctc ggggttatac
    43381 agaaccgtga gattgagatt cagattttgt ttctgatagt ccagcgcccg ttcaaaagtg
    43441 gcatcccggg actggtcatc gatgatcagt atttcataat cgtatttatc cagatctttt
    43501 ctggggatgc gcgccagcac ttttgctata tgtctttccg cattgtacgc gacaatgaaa
    43561 atcaagagcc tcgttcggtg cggtgtcatg gtatgaataa aagaagtaaa attaaaataa
    43621 ttaaatatat ttaatttccg ttttcagccg gcccgaccgg taatgcatcc tgtatgaaat
    43681 cccatccggt cccgccccgt tccagccgcc cctttccagg ttttatttcc atcctggccc
    43741 tcttcctgct gtatgcagtg gtctacggat acgtttttga tgaaaaaata aacctcaacg
    43801 gggacaatat ggggtattat gcttcggcca aaaatatcgt tcaggggaaa ggcttttcct
    43861 atgcctacga catttttgcc acccggctca atgaacccat cggttatccc ttgctgatcg
    43921 cagccacgat gctgatcagc gattccatga atttgatttc cctggtcaac ggagtactcc
    43981 tttttttaag cattcttttg cttctgggca ttgccggcag cctgggcatt tccaggctga
    44041 tccagggcct caccgcgctt tatatcctgc tcaacccttc gctcatgcaa tatgcctaca
    44101 tccatatgag tgaaggcccc tttatttttt tcagcctttt ggccattttt ctctttttaa
    44161 aactcaaacc gggaatcccg tatactaaac agccgctttt ttacctgatg gctttctccc
    44221 tcctggctat gtatttcacc cggaccgccg ggattattct gctgatcggc tttctgctct
    44281 atttgcttta tgataaaaaa tacctccccg catccgtgct cgccttcctg tttcttgttt
    44341 ccctgggaac ctggagtctt tatgcccccg cttccaaccg ttacgtccaa caggtcctga
    44401 tggtcaaccc atacgatgcc cacctgggca agctgacgat taaatcaggg atccaacggt
    44461 ttggcgcaaa tcttaatcgg tatgccggta aggaagttcc tacggtggtt ttcccgttcc
    44521 ttgatccgga accgaacaaa cctgccaagt ggcctcatta tcttatcggg gtgggcatcg
    44581 cggttttgac tttaaccggc ttatggtata aatttccaag ggatccgcaa cggcttttga
    44641 tttttttata cctggccgga tcgctggcga tgttgctgat gtggcccagc caatggttcg
    44701 gcagccggtt tatcactccc ctggttcctc ttattctcct gttttccatg aaaggactcc
    44761 aggacttttc gtccctgatc gttaaacgca cagggatgcg gaccttttcc tatgtgccct
    44821 gcctggcctt actggcggtg cccttcttca ttggaataaa acccagccgg tacaacatcg
    44881 gttccctgcg cgatgctcac aaatatgcgc agtccaagta tccgccagcc tatcagaatt
    44941 tttttaatct tggaaaatgg gccaaaacaa acctgcccgc tcagtccgtc gtggccagcc
    45001 ggaagcccac cctcttttat tatacttacc aggggccgtc ctgctactac cccacaaaca
    45061 gcaaaacgac cgtggaattc atgaatggat tgcgggaatc cagggcaggc tacctggtca
    45121 tggaagaact gggctataag accacccctt atttatcctt ttttatcaat aacctgcctc
    45181 aacatttcca gctgctccac accattccca atccggacac caaaatctac aggatccttt
    45241 atccccaatg attcaatgca tggagcgatc aatcaaaaaa tttgtattta aagatgcaat
    45301 aaatggcttt tagcccatcc ttccagttta tttttttccc ttcttcgtag gtccttccgt
    45361 aataggatat acccacttca tagatccgga ttccctggat ttttgaaatc ttcgcggtga
    45421 cttcgggctc gaagccaaac cttttttcct tcagtttaag cgactggagc acttcgcggc
    45481 ggaacatctt gtaacaggtt tccatgtccg tgaagttcat gttgttgaac aggttggaca
    45541 ggaaggttaa aaatttgttg ccgagggtat gccagaaaaa gagcaggcgg tgcggcctgc
    45601 ccccgataaa gcgggaaccg tataccacat cggcatgacc gcgcaccatg ggggcaagca
    45661 acaaattaaa ctcctgggga tcgtattcca ggtccgcatc ctggatggtg atgaagtcac
    45721 cggttgcttt ggctatgccg gtatgcaagg cggcaccctt gcccgcattt ttctcatggg
    45781 aataataaaa aatattgtcc ggattatttt tttcaaggta tccgaggatc acctcctgcg
    45841 tatggtctgt gctcttatcg ttgaccacaa tgacctccat ggccaggccc ccgatcagct
    45901 gcacttcgcg caatttatcg aggatccgcc caatggtctt ttcttcgtta taaaccggga
    45961 ccagtactga cagtttattc atatccttgg ttcaacaaaa gcgtggagga attgagatcc
    46021 ggaagaccgg aggaattgcc ggatcccctt tccggtaatt tctttgatct ccctagccga
    46081 ccgctaacaa atgtaaatca aatcatgaaa ataccaggca agcggagaga ggagggaact
    46141 caaatatcca ccttggcgta cttcgcatta gcttcgataa actgcctccg gggttccact
    46201 tccgacccca tgagcatgga gaaggtccgg tccgcggcaa ccgcgtcttc aatactgacc
    46261 tttttgagca gtctttcttt gggatccatg gtggtcgacc aaagctgatc ggcattcatt
    46321 tcacccaagc ctttgtagcg ttggattttg acggaagatt cattaccgcc cttggccagg
    46381 gtttccacgg cctttttccg gtcgtcttcg gtccagcaat attgttgttg tttgcccctg
    46441 gataccaggt acagaggcgg cgccgcaata taaatatatc cgtgttcgat cagggaacgc
    46501 atgtaccgga aaaagaaagt aaggatcagg gtcacgatat ggctgccgtc gatatcggcg
    46561 tcgcacatga ttacgacttt atggaaacgg agtttgtcca cattcagccg cctttccccg
    46621 tctttctctt cgatccggac cccgagcaca ttaaaaatgt ttttgatctc ctcgctttcg
    46681 taaattttat gttcgaacgc tttttcgaca ttgaggattt ttcctttcag ggggaggacg
    46741 gcctggaaat tccggtccct cccggtcttg gcggtgcccc cggcactctc tccctccacc
    46801 aggtaaattt cacattcatc cgggcgccgg ctcgaacaat cggcgaattt atccgggagt
    46861 gaaatgctgc ttatgccttt tttgccaatg atgagttcac gggcggccct ggcggcttcc
    46921 cgggcccggg cggccagcac cactttatcc ataatccgct tggcttcgcc gggattcagt
    46981 tccaggtgat ttttaagcgc atcgcccact gcgcgggaga cgacggtagt gacttcgttg
    47041 ttacccaatt cccccttggt ctggccctta aactgggggt ccggaatttt aacggaaacc
    47101 accgcggtga gtccttcacg gaaatcttct ccggatacct tgaatttaag ttttgaaaaa
    47161 taaccgttgg attcgccgta ttccgaaaag actttggtta cggccatccg gaatccccgg
    47221 agatgggtcc ccccttcccg ggtattgata ttgttcacga agctgtaaat gttctcattg
    47281 tagccgtcgt tgtattggag ggcgacatcg atttcgatat tgtcttcctt acctctgacc
    47341 gaaatcggtt tatcaatcag cggcgtcctc ccttcgtcca ggtaggttac aaattccgaa
    47401 agacctcctg atgataaaaa atcctcgcgg ataggctggc cgtcatcatc ctgttcccgt
    47461 tcatcgatca aggtcatctt caggccggga ttcagaaaag acagttcatg caaccggttg
    47521 gccagagtat cccgcttgta ctccaacacc tcaaaaatcc cggaatcggg tttaaaagtg
    47581 atgatggttc ctgtttctga ggactccccc gtaacgatta cttctgtttg gggtttgcct
    47641 ttactgtatt cctgtaccca gattttaccc tccctgcgaa tttccgcccg caggtattca
    47701 ctcaatgcat tcacgcagga gacgccgacc ccgtggaggc ctccggagac tttataggta
    47761 tccttgtcaa atttaccacc cgcatgcaga accgtaagca ccacttccag ggcggatttt
    47821 ttgagctgtt catggtatcc ggtcggtatt ccccggccat tgtccttcac ggtgatggaa
    47881 ttgtcagggt gaatggtcat tacgatttca ttgcaatggc cggccaggtg ttcatcgatg
    47941 gaattgtcta tgacctccca gaccagatga tgcaatccct tggtatccgt agaaccgatg
    48001 tacatccccg gccttttgcg gaccgcctcc agtccttcga gtgcctgtat attttttgcg
    48061 gtatattcac ctgctttttg tgtcatttat cctttccctt ttttcaggtg caaaggtacg
    48121 aattataagt cagaaaggat caggaaaaat ccaaatttta aacgcctatt ttaaatgtaa
    48181 aatattgtaa aatagaggaa tatatattac tactgtctgt tatgcggtga gaaatgaaaa
    48241 aaacctttgt ttatgccaaa gaaagcctgg aagactttgt gaaaaccctg attttccaat
    48301 accccttgaa caaaaaatgg gcctggtacg gccaaatggg atcgggcaaa acgaccctca
    48361 ccaaatcgtt tatacgtcag ctgggttccg tggacccggg ttcaagccct accttttcca
    48421 ttgccaacac ctacctgtcg gccacgggag aatccattgt gcatcttgat ttgtaccgcc
    48481 tgcattcggg ccaggaggcc tttaatgccg gaatctatga attgttgcag gagccgggct
    48541 attgttttgt ggaatggccg gaacggatcg aaaactggct ggatgaggag tgggtgaccg
    48601 caaggatcag gatccttgac ggggactgcc ggttggttga cgtcttttcc tgaatttatt
    48661 cgtcgtcatt taaaaacaag accggcaccg ggctgtcgat cacagtttta ggggtggggc
    48721 tggattcaaa aatatattcc agcaggcctc ttttcctttt gattacacat aacatgtcca
    48781 tgctttcccg ggcctggatc atggcaatcc cttcagtaaa actgtctgcc tggaggtctt
    48841 caaaagtgaa agcccggttt tcaaaaatca attccggaga ataggcaatg gtccctgttc
    48901 cctggcccgt gaggtgaacc gcttttaccg gaaccttata cgcatcgctg agctcaaaca
    48961 agggaggcag tatatcgaaa aggcttttat tcaacttcct gaacatgaag aaaatgttct
    49021 gcactttcct ggcctggtaa cttttgggga tcacgatgac cggaatattt ttaaacttga
    49081 tgatgctgcc ggccactttg cccaggaaat aattttcctc attgtcatct ccctgagcac
    49141 ccatgatgat caggtccacc ccgctgattt ccgcctggcg ggaaatgacg gtataaggtt
    49201 cccctttagc gatcttagtc aggatctgaa tcgaagattt tttcttgtat ggcgctacga
    49261 atttctccag ccggctgatg gcatcctgtt ggatgatctg gtttacggaa cgcaagccca
    49321 cggttttttc cataaaatca atcacgttga ggaccaatat cttgctgccc agtacttctg
    49381 ccgtctggat tgcaaaatcc agggtttgcc gggtaccctt gctgaaatca acgggaacga
    49441 gaattttttt caaagtacaa tgaggatttg aaccaaattt acgacatctg ctgtaaatcc
    49501 aggcccgcat accctatctt tggtagcctg attcaagaat gccccatggc ccgacacacc
    49561 atcacgattg aatattgccc caaatgcaac tggatgctgc gcgcggccta ttttgcccag
    49621 gaatttttaa gctccttcac agagaatatc caggggctca ccctggtacc cagtgagcaa
    49681 aacggcagtt tcatcatatc cgtggatggg ataaaaatat ttgaccgcat cgaatccggt
    49741 ggtttcccgg aaattaaagt tttgaaacag atggtcaggg accgcgtcgc gccgcaccag
    49801 ccgctcggac acgccgacac cccttcccgt ttgaaatcct gaaatttgaa ggcagtcatt
    49861 ctctgtggcg gactcagcag gcgaatgggg accaacaagg cccttatgca ttggaagggt
    49921 aaacgcctgg ctcaatatgg gatcgacctg gtcaccgcat tgaatctgcc cgttttttta
    49981 tccgtaaagc aggcagaccg ggataccttc caaaaggttt tccagggatg cggtattatt
    50041 cccgatgaga atgggttaag cctggagggc cctgcaagag gcattctgag cgcacacctg
    50101 cttttacccc aggaagactg gatggtcctt tcctgtgaca tgcccttgct gactccatcc
    50161 atgggacgcg atctccttcg atcccacgga gtacacccgg aggcggaagt cagcctgttt
    50221 tccagtcagg gcatttatga accccttttc ggcatttaca cttccactgc cctgggtcac
    50281 tggcttcggc aggatgaaac cggcgcatgg tcatcatcga gtatgaaata tttactcagc
    50341 cgggttcggg tacgggccat tcccgcaaag gacagacaga ttcccttttt cctgaatgcc
    50401 aaccgccctt cagacctgga tgccggtgtc cgggaagtat aattcccgtt ttttcttaag
    50461 ttcaaattcc cctggaggcg cttcacaaac cgaacaccgg tatccccagg gcaggacttc
    50521 cggcgggatg aactcagcca ggctgagaca ttgtctgcat tgaaaaaaat actcaggttg
    50581 accagcatcc gatgaatcaa ctcctgcacc aggaatcagt ttttcctgcc aaagggggtc
    50641 ttccagcctg aaccggtaat aagaccggat acaggcaatc aactggtccg ggaccaggta
    50701 tctgaacagg gatttcccga tcaggtattc actgcgttcg ttgggattaa agtctttggc
    50761 cgcaaaaatg tcgtacaccg gtataaagtc cagtccaagc aagtgaaaca ggtatttttt
    50821 cctgatcagt atggagctga atatttctgc cttgctccgc gtcttgatgc caaaacaaat
    50881 acctgtagtc cggatatcgg catcgttgaa tcgttttacc aggaaatgct tcaaagccag
    50941 gccggaaggg ctgtcatcct cgacctgaaa gttcaattca ttgagggcat gcctgagatt
    51001 gatctggtac cggttgagca gccggatcca cttcggacgg tctttgtctt caattccttt
    51061 gataatcaat gacttccagg ctgtggaaca gatctgacca attcttgttt cctgactgag
    51121 caatccaagt tcatggagaa atcccacatc aaaaaattca tccctccggt aaatacccaa
    51181 ccaatagtgg tcctgaaagg cattgaagcc ttcataataa gaaagcgtaa aaggcggaaa
    51241 atccggctcc gcttctttag gtatggtggt cccaccccgg cttttcatta ccaactgtaa
    51301 ctgctccccg gccaccttac attgaacttc attgaccggc cggaaatcag acatcctgga
    51361 gtccagctgc cgggaaaagc gcatcagttc attggtgtaa aacaattcct tccatgcata
    51421 aacggaattg gtcccgggaa agcggatgta caaatgccaa tagtgaggaa tcgcagaaga
    51481 aatccagttg acatttcctg taaaaaatgg ggtaaaagac tgatggatat cgcaaatatt
    51541 gatcttgagc ctgggccgga actgcaattc atcgagaata tccctgtaaa ccccttcggt
    51601 cagccaggaa gcggggctaa aaatcccagc ggcgggataa gaactcatga cattgggata
    51661 agcgtccgaa tccgcttcat acaggattcc cgctttctcc agttgccggc aacagtaatc
    51721 gcaataaatt ttatgaacgc ggaaatgcat ggactgacgg agtccgaatt tcagacgttc
    51781 cgttttaact tcctttaaaa tacccgtcag ggtaaccaat aaaccggatg gccatatccc
    51841 accggggccg ttgatcttga actggacgta gtttttgaag ataccggtta agttcatggg
    51901 tgaagagttt ggtgcatcaa gccaaattcc cggagagccg gcacaaagtt tttactttca
    51961 tgacccggcc gaatcaattt gcccagggca aatcgttgca acaccgaaag tccttcccat
    52021 tgttccggtt caatccgatg ccccaggcca ttggcccttg aatttaccag gtcatataaa
    52081 tccgggccta attgagcatt gaccaaacca tccagccttt ctgcctctcc ccatccgtgc
    52141 cgtttcagga gggattgcag gctggcttca tatcgctcct tttcaagccg gtggttacaa
    52201 ggaagggtag ccagcagatt tctttcttcc ggggcaaagg aggaccattg ggcaaggctc
    52261 agtttgatgc cggcgatatc caatttgaac cggatgatca tgggtatgca ccggatctgt
    52321 tcttcaacaa attctttttc aaactcgaac accacgaagt ctgggttgaa cgattggata
    52381 ggtttggatt cagaattctt catatgcata gtggggtgtg atgttgctct ctggccttaa
    52441 ggtgcgcttt caacaggttc ttgatctccg gtcggcagct gccacagccg gtaccggcgc
    52501 cggtcaactg gcaaagctgg accagatcgg tgcatcccgc attcatcttc tcttccaggt
    52561 tgccctctcc tacctggttg cagctgcaga ccagtctgcc ggaaaccggc tcctgctttt
    52621 tgccgcttcg caataaatcc attctctttt cgcttaattc catccgccct gcaatcagat
    52681 cccggaattc aaggaattct gatttatccc caatcaggat agcccccacc aaacggtcat
    52741 cgcggacaat acatttttta taataccgtt ttgccaggtc cataaaaacg atctcctcac
    52801 atccttccga accgggagga atcagggcat cccccaggga acaaaggtca agcccctgga
    52861 ttttcaggat attcatgaac agggaacccc tgtaaatgct tccggtatct cctgaaaccc
    52921 agccggcgag gatttgcgcc tggtgttccg ctgccgctgt ggagccataa ctgatgccgc
    52981 ggaattctgc tacctccccg atggcataaa tattttcatc gctggtcctc aggtattcat
    53041 ccaccaccag tccccgctgg cattccaggc cgcagtctct cgccagctga tcattgggca
    53101 gggtcccaat ggctaaaatg acggacttgc attccagggt caatccgctt ttcagcctga
    53161 ccccagtaac ccgtttttca cccaggtatc tttcgatttc atcgttataa tagatgtcaa
    53221 tacccctttc ggtcaaagcc tcatggagca cctggctgcc gagagggtcc aattgccggt
    53281 ccatcagtct ggatacccgt tgaatgataa ccgttctggc tcctttttct ttcaggctgg
    53341 cggcaagttc aatccccagc agccccccgc ctaaaatgac aacggtttct tccgccacat
    53401 ccacaaagga tttaaaggca tcggcgtccc ggcgggaccg catggtaaaa ataccctcca
    53461 tgggcggcaa attccggatg gtggccgccc tgctgccggt ggctatgacc agcaggtcat
    53521 aatcgtgttt ttgaccctga gcgtcggtaa ccgatttatt aaatttatcg atggcggtga
    53581 tggccaggcc gcgatggaat accgtgtgcg gtatattttc ttcttccctg tccatcttga
    53641 ccaggctgct ccagggtaag gcacccgtta tataaccggg gagcagcacc cggtcataaa
    53701 agggatggtt ttcatccccg aatatttcaa tccggtcttg tcggttgagg gcccggtatg
    53761 atttcaggaa tccaaaggcc gcggccccgg ctccgatcag gatgattttc tggaccggtt
    53821 tattccaaac acggacattc actgcggcgt atttaaagtc cggttctttg ctgaccgggt
    53881 cgagcaattt tgcggtgagg ttgtttgccc ggagcaggtc ctggttcccg gctttacccc
    53941 agtgcatcgg cataaataca accccttgcc tcagctcagg gctgagtttt gctttaaccc
    54001 tggcctgtcc ccggtgattg attacttcga ccagcttccc ttcttcaata ccccgggcca
    54061 gcgcgtccaa agggtgaatt tccacgaatg cttcccggaa atgctgattg agtttgttga
    54121 cttttgaagt tttgctccgt gtatgccatt ggtcccggat gcgaccggtg gtcaatatga
    54181 gtgggaaatc ccgggagagc ggttcagaat ggttttcttc atcacaggcg tgaatatgcg
    54241 ccttgccgtc agccgtataa aagacctgat cctgaaacaa ccgttccgtt ccgggaaaac
    54301 cagcattgtc ctccagcaat tttttcggat agggccactg gaccgttctc tgttttttaa
    54361 ggatgttata atccaatcca ctgatgtcga tacgggtacc cgctgtaagc tccgcatgtt
    54421 cttcaaaaat ctgagaagac gagctgaaat caaatccatg aaatcccatt ttccgggcca
    54481 gccggcagat gatttcagca tcgggaaggg cttctcccgg cggatccagt actttttcca
    54541 gaaggccgat atgacgtcct gcattggtca tggtgccttc tttttctgcc caggctgcag
    54601 caggaaggat gaggtcagca tattcaaggg tttcagcttt gttgctgatt tcctgaacta
    54661 cgacaaacct ggcttttttc agagccgctt cggccttccg gacatccggc agactgacca
    54721 gagggtttgt agcaatgatc cagactgctt taagcgcacc cgtatccagc gcatcaaaca
    54781 tttcggtagc ggtgaggccg ggtttcggag caatggttcc tggcgggatc ttccagaacc
    54841 gttccacttc atttcgatgg tcttcccgga ggagatcccg gtgagccggt aataaattgc
    54901 ataagccgcc cacttccctt ccgcccatgg cattgggctg gccggtcagt gaaaaaggac
    54961 cggtgccggg tttgccgatc tgaccggtga tcaggtggag attgatcagg gaaagatttt
    55021 tatttactcc tgaaacgctt tgattcagac ccatggtcca catggagagg aaggccctgg
    55081 ccctcccgat ccggatggcc gcctgtcgta tatcctccgg gttcagcccg cagatccggg
    55141 ctgcttcttc cagggatctt ttgaatacgg attcacggta ttgttcaaat cctctggcat
    55201 ggttttgaat aaaagaggta tcgatatacc ccagttcaat gagggcgcgg cccagggcat
    55261 gatggagggt aatgtccgtc cccggtcgga tttgcaggtg caggtctgcc aggtcgcagg
    55321 actgggtttt gcgcggatcc gtaacgatga cggtgagggc aggatttttc tccttggccc
    55381 tttccaccct ccgccatagg atggggtgac accaggctgg attggccccg gctacgaaga
    55441 tcacatcgca agcctccagg tcatcgtacg aaacgggcac acaatcttct cccaggctca
    55501 ttttataggc caccacggca ctgctcatgc acaaccggct gttggtatcc aggttattgc
    55561 tgccgataaa acccttcatc agtttgttga tcacataata ttcttcggta aggcattgac
    55621 cactggcata aaaagctacc gcatccggtc cgaatttgcc ggtcagggat ttaaaaaccg
    55681 cagcggcccg cgacaaggcc aggtcccagg acaccctttg acggggaagg ttccggtggt
    55741 acctcatttc aggaaaaagc aggcggtcag atttatcatt ggcagtatag tggaggttct
    55801 tccccttgct gcacaacatt ccccggttga cgggatgccc ggggtctccc tccagttgta
    55861 tccggttttc cccttctttc cggatgacca ggccgcatcc gaccccgcaa tagcagcagg
    55921 ttgattgata tttctgaggt ccggtgtggg gtgccctggt cataattaca atacatttgg
    55981 gctgagttcc atactcaggc ggggctcagt aagcggcctg gcaaaacggg tcaggaagac
    56041 caggaaggcc acggccatca ccgtcatacc tatataattg aaagcgtcca ggtaggtcag
    56101 gcttctactt ttaaataaaa agccgaatac catgcccccc agattacctc ccgcaccgac
    56161 gataccactg accataccca cctgggcggg cttgacgaag ggggtaatgg cataggtcac
    56221 tccattggcc attttcagga agagggcaaa tccgatcata gataaaatgg ccaggctgaa
    56281 tgaaacggca tgggcaaaaa agatcaggcc caggccttcg aacatcagaa cgcccgacaa
    56341 tagcaatccc ttccctctta acccgtaaag ccgcccggct ttgtcggcaa aaatccctcc
    56401 cagcgcccgg gcaaataggt tcatgaagcc aaacatgccg gcccaaaaac cggctgattg
    56461 ctggctcaaa tcgtattcct ggacaaaatg gagggcggct acattatcaa aggtgatttc
    56521 catgccaaag cagaccgcat atgcaagggt aagcgcccac acccgccaat ccttcagcac
    56581 cgaataatcg gttttgattt taccgggttt ccttctttca agatcttcat agttccccgc
    56641 aggggagtcc tgcgtataat ggtaatagaa aaaagccatg accagcatga ggagacccgg
    56701 gaagatcatg gcatacctcc atgcttcctt gtccgtaaag ccgaatccga ccaggagcgc
    56761 gaagatgagg ggcatcacca tattggtaat gcccccgccc agattgcccc agccgccggc
    56821 taccgcattg gcggtcccct tgatccggtc atcaaacatc atcgaagtat ggtactgggt
    56881 gattacaaag gaggctccga tgatgctgat cgccaggcgg aaaagaagaa aagaactgta
    56941 gtcccgggct aagccgactg ccatgaccgg taaagcgccc agcagcagga gtgcggtata
    57001 ggtttttcgc ggcccccagg tatcgcaaag ccggccgatc agcaagcggg ccacgatggt
    57061 accggatacc gaagcgatca caatattgcc aatctgcgat ttatccaggt tcaggtccgc
    57121 cttgatggcc ggcatcagcg gagccagtcc gaaccaaccg aaaaaacaaa caaagaaagt
    57181 cagccaggta atgtgaaagg tgcgcatctg cacccctttg aaggaaagca ggttgagtac
    57241 ggataaggat ttttgagaca tggttttttt attatggggt ggatttttgc aggaaaaacc
    57301 cgggacggat attaaattgc acataagccc aggcaggtgc agactggtac gtacccggag
    57361 gtaacccttt ggcatattgc agggaaggac tggctcggta ataacagaac ccggattcga
    57421 tctgggtata gggattgagg ctaaaggtgg tgatccagtc gagttccagg ccgagatatt
    57481 ttttcatggt attgccgcgg tcgtcgggga agtgatgggc tgtatggaac cggtgcaggt
    57541 caagcccggt ggacaggttc tctttttttg aaaccagttt aattttcagg tatccatcct
    57601 gcagtccagc aagcggagac ccggtggcta cataaaaaaa atccatatag ccccaatgcc
    57661 ggtgaggcgt gccgtacaaa ggatcgaagc ggtggctgtg gcccgggcgg gtgtggtcat
    57721 catcaccgga taaggcatca taaccggctt ggatatggag agggccccat tggagggagg
    57781 tcgaaaaaga acagtgccag gctttcaggc ggaggtcgtc ccggtcccgg ccggtctgat
    57841 gataaattgc tgcctggagc cccacccagc cgagacccga aatccggcca aggtgctggc
    57901 tgatcgtgaa accgatggtc gttctggaga gtgtgcggta atcaaaatca tcctggtctc
    57961 cgctttttat aaatctcctg ccgtagagat aggctccatc caggattatg gaatccagct
    58021 tgtaacgcgt aaaccgatct ctaaaaaaaa ggaaagacca ggtggagagc ttgcttttgc
    58081 gggaaaagta taaactcgaa aatgatttat aatgttgtga aggggcgttg gtaccggacg
    58141 gatgaatata gacagggagt cccgatggag tgcttaatgc gttgacctga ccgcccaaag
    58201 tcagggggac aaggcctgca ggcaccgata ccggcgtgcc cgctgcattt tttattgtag
    58261 cgggcacaga gcccgggaca taggaggttg ccctaaaacc ttctgcattc tggttgaaag
    58321 cctggccgat ctcaatctgc cattttttaa tccctaattt gagaagggcc atgtcatggc
    58381 gccgtccttg ctgaagccag tccaggttac cgatgagttt ttggtcatcg taactgagtt
    58441 cttgcctgcc gagtttgagt ttcacatatc caatcaatgg gggtccgtcc aaagaatcca
    58501 tcgtcttcgt caggaggatc tccgcccagc cttcgtgaag catcagcctc gaaccatcgg
    58561 ccggggcaat ggtggacccg tcctggcccc agacccggat atcctgaacg ctggctccaa
    58621 tgccaatccc tgaattttgg taattcatct ggaggcgggt acgctgggaa gtaaaaccag
    58681 ccggcctaga gccggagggc aggagattac cgtaaccatg tctgatttct gagcgggtac
    58741 gaagttgtcc cgctatggaa aactgagctg gtacagacaa aggcccgaaa agcaagacgg
    58801 ccgcgcatgc aaaggaagct attgcttttt tgcaatacat ttgaacatgg attaagttgt
    58861 tcgtcaattt aatttcacca ggaccccgtc tcgactccta catcagccgg ggtcttttta
    58921 ttttattttg atatacactt tgttttcatc cagcctgacc ggatagacct ccaggctttg
    58981 ttcatttcca ttcaggttta tccccgattt gagagagaag gtatttttat ggaaaggaca
    59041 ggccactttg ggttcatcgc ctgcactgcc gatcaagccc cggctcaggg ccatctgctt
    59101 gcggtgcgga cattcattct gcgtggcata ccattcattt ctgcgggcga aataaaacag
    59161 ggctatttgg cagtccccgt attttataca aacgcctccg tcttccggga cctcttcctt
    59221 gtaacaggca aaaaaccagt gttcgtccgt ccgcccgcta tctccgggct ctgcagattc
    59281 tcctatttcc attccctggc ttttttttgt tcccgcatag gttcaaagtc cagggtggga
    59341 tccttttcac ccggtgcatt gacaaaatga gtaaaacgtt ttcttatttc aggggtctcg
    59401 atggccactt tccactcaca ctggtaggag gccacatgct ggtccatttc ctgttcccat
    59461 tgagccgcca ggcccaggct gtcccggatc acgacattcc ggaggtaatc gatgcctccc
    59521 tccattttat tgagccaggt ggccgtgcgg gtcaacggat cagcggtttg aatataaaac
    59581 atcagaaacc ggtcgatata ccggatacag gtctgctcat ccaggtccgt agccaaaagg
    59641 agggcatgtt ggggtttgct ccccccattc ccgcatacgt aaaggttcca gcccttttcc
    59701 gttgcaatga ggccaaagtc cttgctctgg gcttccgcgc actcccggat gcacccactt
    59761 actgccgatt ttattttgtg gggagccctc agcccgcggt aacgttcttc gatccggatg
    59821 gcaaaactga cgctgtcatg caggccaaaa cgacaccagg tgctgccgac acagcttttg
    59881 accgtccgca gggctttccc gtaggcatga ccgctttcga atccggcggc gatcaggtct
    59941 tcccagatca ggggcaagtc gtggagatgg gccccaaaca gatcaattcg ttgtccaccg
    60001 gtaatcttgg tatataaatt gtatttttta gcggtcaaac caatggccac caatttatcg
    60061 ggggtgattt ctcctcccgg aatgcggggt actaccgaat aactccctcc tttctggata
    60121 ttggccagga agcggtcatt gctgtcctgg gccgttccgt ttccggaccg gaggggcatt
    60181 tcattccaaa gggaggcaag gatggatgcg atcaccggtt tgcagatttc acatccatcc
    60241 tggctgccaa aacgggacaa cacttcgtta caggtcttga tttggtacaa tttcaccagg
    60301 tcaaacaatt cctgccggct gtattcaaaa tgttcacaga tccggttgcg aatgtatttc
    60361 ccttcggcct taagggtttc cgtcatcaga tctttaagta taggcacaca tcctccgcaa
    60421 ccggttccgg ctttggtgca tcgtttgatg gattccacat tttcacattt ctcaaatctt
    60481 acggcatcgc aaatagactt tttggagatg ctttcacaac tgcagatcag ggcttcatcg
    60541 ggcaggctgc caagacctcc tgaaacagca ttcccccggt cgcccaggat gagatcttcg
    60601 ggctgagccg gcaggatgac tttattcctt accgtctgga ccagaaggtt gtattgagag
    60661 gcatccccga tgagtatccc gccgagcagg ttttttccgt ctttggagat attgatgcgt
    60721 ttgtagaccc cctccggtcc atgatgaatg gtaatgacat ccgcctgaga accgttggca
    60781 aatgggtcgc caaaacttgc cacatccacc ccgataagtt tcagccgcgt actcatatcg
    60841 tatccggcaa agggctttgg ctgatccagg atgtggcaac aggctgcttc ggccatatca
    60901 taacccgggg ccaccaaccc gtaaatcatt cctttgtgca aggcgcattc tccgaccgca
    60961 taaataaaag gatccgaggt ctgcatgttt tcgtcaacca caatacctcc ccgggtcccg
    61021 gtttccagtc cggccatttg tgccaattcg tcccttggcc ggataccggc cgaaataacc
    61081 aacaagtccg tatccagacc ggtcccgtca ctgaacaata attgcctgat ccccggatct
    61141 ccttccaagg cgaccgtatt cttgccgagc aggatattca gtcctagggt tttgagtttt
    61201 tccatcagga tttgactccc catttcgtca agttgtcggg gcataagccg ggaagcatat
    61261 tcaatcacag atacctcccg gagtcccagg tcaagcaggg ccttggccgc ctccaggcca
    61321 agcaggccgc ctccgatgac cgctcctttc tgggattttg cagcccggga ttctatgagg
    61381 tcgaggtctt ccaggttgcg gtacaggtat acgccttttt tctcaatacc ttctatgacg
    61441 ggaacaaagg gggcagaacc cgtagccaaa atcagtaaat cataagggac gcttttgcct
    61501 ttgaaggaat ccactgtttt cgactgacgg tttatctggc aaaccgggtc ccccagatgc
    61561 agttgaatgc cctgtccggc ataccattcg gcaggagcca ggtaaaggct ggcttcttcc
    61621 tttccggaaa aataatcgct gaggtgaacc cgatcatagg caggcctggt ctcttcgcca
    61681 aacacggtga tctgaaaacg gcccggggcc cggcccgtca gtttttcaca gcatttgaag
    61741 ccaaccatac cgttgccaac gatcacaatt ttcatagacc aattgtttgg gttgattgca
    61801 aatctagttg aggcacaggg ggctggtggg ataaaaaaac ccttttattg tgtcatcgaa
    61861 tcgattgata cggttttaac taaaatttga atttttcatg aaataggttc aattattggc
    61921 ttattttgct ttataaacaa tatatctttc acttttactt tgtaattcgg ttaaaatgga
    61981 ataaaaaata gaaaatatac catatataag tttactgcat atataggttt aaaacgagag
    62041 gccggttgtt tttccttgcc ctgacctcct taagctttca gattaaccgg tttattgtga
    62101 tgcccagcgg gataaggagg gttcaagcta taaccctggg aatactctgg aagtattggg
    62161 tccgaaaggc cggcagaggt cctgaaaact caggcattaa gtaaacgttg acggatcgcc
    62221 tccacgggcc ggatgcaaga cctggtaaaa acccggtcag ggagagcaag gctatcattc
    62281 tgatcccaga gcatccaccg gtttgtgaac aggaccggat taggatacaa atcctccggg
    62341 ggaaaaacaa aaaaccccgg gttaccgctt tcgcgctccc cagggtttcc cgatagatgt
    62401 agcaataggt tttataactt ttctccgtgc tgcgaaagat ccaggccctg gaattcttct
    62461 tcgtcatgca cccgaagggg agaaatggcg ttggtaatcc tcagcaggat ataagtacca
    62521 aagaaggcga aaatggaaac tgcaatcaaa gccagcagat ggatgaagaa caatttggtc
    62581 tctccgaaaa ataatccgtt gccggtggta ttggccgggt tgatcgcctg gctggcaaag
    62641 accccggtga ggatcatgcc taccatgcct ccaaccccgt ggcaagggaa tacgtccagg
    62701 gtgtcgtcca gagtggtccg gctcctccat tccaccacga ggttgctcac aatgctggcg
    62761 acgatcccga tggtcagtgc atggggtatg ctgacaaatc ccgctgcagg cgtaatggcg
    62821 accaggccaa ccaccgcgcc gatgcaggcc cccatggcgg aaggtttgcg gccgcgtatg
    62881 gattcaaaga taatccagct gaaagcggcg gcggcagatg cggccgtagt cgtagccatg
    62941 gccgtagccg ccagggaatt ggctcccata gccgaacccg cattaaaacc tagccagccg
    63001 aaccaaagta aaccggtacc cagcatgaca taggtgatcc gggccggttc gagccccgtc
    63061 gcctccgacc tgcggcgcaa aaacagggcc gcggcaaggg ccgcccaacc cgcgctcatg
    63121 tgcaccacgg ttcctcctgc aaaatcgagt acacccaaac caaaaaacaa gccttccgga
    63181 tgccaggtaa tatgcgccag cgggctgtat atcagcaaag accaaaggat gataaacaag
    63241 acataggaag aaaagcggat ccgttccgca aaggccccgg taatcagggc cggggtgatg
    63301 atggcgaatt tcagctggta aaaacagaag agcaccaggg gaatggttgg ggccagggac
    63361 cagggtttct gagccaggat atcatccatc atgaaatagg ttgcggggtt gccgatgatg
    63421 cctccaatgc tgtcgccgaa agccaggcta aatccaacca ctacccaaat gatgctgatc
    63481 acagacatgc tgataaagct ttgcagcatg gtcgaaatca catttttctt tctcaccatc
    63541 cctccataaa aaaaggccag tcccggggtc atgatcaata ccagtgcgct ggcgcagatc
    63601 atccaggcaa tatcactgcc taccaggtca tctccggcat ataccgcctg gggaatgctg
    63661 ggatggatca aactcaataa gacaattaac agtagaatcg aaaacggaat ccatgcaagt
    63721 cgtttgttga aagacatatt cagtactttt ttttgattag gactcaaatc tataaccggg
    63781 aagagccccc agaaatccaa aaagtcccaa gatttgtaat tttcattttt tcaagaatca
    63841 gtcccatttt aaggtatata gtgtgaaata tttacatttc ttgtaaaaat gacttctaaa
    63901 tccaattaga aatgctgaaa aatgtgtgtt ttaacgaaaa tcaactttga aagaatacac
    63961 ccggatccac ccctaaagat ccgggggatt aaatgctctt ccggagcctt cccggtaagc
    64021 ttttttggtg gccacccgtt gtttttgcct atccaggatc aataaggcga gggcatgcgc
    64081 tgcatccgcc tgggattgaa gcgccttcct gattttagac tcctccacat ccagccggta
    64141 aaggaaactc catagcaaat ccggttgatc agccagcagc tggcttaccc ggtcttccaa
    64201 taagctcaga aaagccgcct cgtccaggaa gggcttttgg ccttccaggt ccgtcatcca
    64261 ttgctccggg agccggtcat ccttttccat gattgtaaaa ttgaaggacg gcatcgatga
    64321 tcgaacggtc atttttgagg cggggtacct tatgctggcc ccccagctta ccctgggatt
    64381 tcatccagct gtaaaaaaag cccctgggca tctcatgtat gatcaactgt tccagggcaa
    64441 ggtttttggt cctttttgcc tcgtaatccg aattcaggct ttgcagggcc aggtccaggg
    64501 accgcgcaaa cgctttcagg tcggaagggc ttttttcaaa ttcaataatc cattcatgcg
    64561 ccccctttcc gcccccgtcc atatacttcg gtcccgctgt aaaatcggta accatacatt
    64621 ggtgctctcc gcaggccttc tccagggcct tctcggcatt ttccacgatg acttcctcgc
    64681 cgaaagcgtt gataaactgg gtcgtccggc ctgcgatctt tatcctcacc gggtccaggc
    64741 cggtgcattc gatgacatcc ccggtatcgt acctccacaa tccgcaattg ttggtgatga
    64801 tcagatggta aggggtgttc agcctgatcc cttccaccgg gacgatattt ccggaatgaa
    64861 ggtccttaaa ctcgtaaaag acggcatggt tcagcaaaag cagcatgcct tccccctgct
    64921 gattgtaccg ggtggcaaaa tacccctcgg aagcgttgta cacctcctgg tacaaaaagg
    64981 attcgctcgg gatcagtttc ttaaatacag gcaggacagg gccaaaattg accccgccat
    65041 gaatgtataa tttcaggcca ggccagatct ggagcaaatt ttccttgccg gtttgctgga
    65101 ggatgttttt aaaaagaacg atgagccagg tcggcactcc ggcaaacatc accaggggct
    65161 catccagaat ttgcccgggc gtttctgcca gtttaaactc ccagtcctgg gccgtggcta
    65221 gttcaatgga gggtacaaaa acctgccggg cataccaggg catattattc aaaagcaagg
    65281 cagatatatc gccacttaca atacccggat actccgccag gcgctccaga ctccccccaa
    65341 tgatcatatt tttaccctcg aaaagcccga ggtttggaaa ctggttgtat aaaatggaca
    65401 gggtatcgct tgcaccctgg atatggttaa actccaggta ttcatgggtg acgggaatgt
    65461 atttactttt tccactggtc gtgcctgaag attttgaaaa catatccacc aggcccggcc
    65521 aaaggacatt ttcttccctc tttcgcatcc gctctatcca gggctgaaat tccgggtaat
    65581 tgcgaaccgg cacctgccgg atgaaggact cgtaatcccg gatcgccgaa aaatgatgat
    65641 tcctgccaaa ttccgtataa ttccccttgt ataatatatc ctgcaacagt tcattttgaa
    65701 aacgtatggg ttccttcatc caggactcca cctgcctata tctgttgcgg aacaatcggc
    65761 ttaaaaaaaa ctcaagcatg ctggctaaaa atagccagaa atcaggaaac gtaatttgtt
    65821 aatcgggtcc ggaaatatgt tgaatcagcg ctggcggttg aagggcggaa gcagaaggct
    65881 ggaaagatgt gccaatcgct gaccatttcc cattgactat tggctattga ccattgactc
    65941 ttgactcttg actcttgact cttgctcacg accccagctt catcctgaat tgctccatcg
    66001 gcaccgtatc cattcctttg gttttggcaa ggggaatgag ctggctcagc agtttgctgg
    66061 ctttttccgc cttgccattc agggcgtaga tccgggcaag ggtaaactgg tgttccaccg
    66121 cggaagacga attgatcaat tttgaaagca actgttcgca gagacccgtc agtttttcgt
    66181 ccgtattaaa ggatttttcc acttcaaggg aaagatggtt gatcagaccc ggatcttttt
    66241 ccacggaggc aggaagtttt ttcatggcct tgtacaggct ctccccatcc ttgacggaag
    66301 tataataatg gatgttggag ttgaattcaa aagcatcgcc ttcttctggc attgctgcac
    66361 ggatctcctg ttgggcacct gtaaggagat caaaagactg aaattcaacg gcttttaata
    66421 ccgttcgatg acccgcctgg atccatctgg attttacctt ttcttccccg aacaatttga
    66481 ccagggcttc tttcgatttg acggcaaggc taaagatgcg tgaatcggtt tcggtagcgg
    66541 cctctaagat aaacctgaga ttatccggtg tggtaagatc cttttggctg ttgaggtaat
    66601 cattggcaat cttaagactg ggttttcccg cctggttcaa cgacttgaca taattgtaaa
    66661 cggtattaaa atcgcgcttg ccggcatcgt attccttggc atagagcgcc gatttgtcgc
    66721 ctttcttcag ggcagatctg cccgcatcca ggaatttggc gggatccatg taacccaccg
    66781 ccttcatcac caactggcct tccccgtcga tatacaacaa ggttggaaaa gccgaaaccc
    66841 cgtatttatc cgaaaaatca aggccttccc ccttttccat atccagcttc acattgagga
    66901 aatgggtatt gtaaaattca ccgacggact tgtccggaaa ggtctttgcg gacatcagtt
    66961 tgcacgggcc gcaccaggtg gtataagcat ccacaaagat caattgcccg gattcttttg
    67021 ctttggccaa ggcttccggc caggtccctt gatgaaaaac gataccttcc gacctgagcc
    67081 cggtccagac gaaaaacacc aggcaaacca ctgttaaata tctcatgatg aatcgtttag
    67141 tctgcaaaaa taaggaaaaa aggtttgaga aaaaggttaa ggttgaggct gaggctgagg
    67201 ttgaggttga ggttgaggct gaggctgagg ctgaggaact gactactgat tactgactac
    67261 tgactactga ctacttattt ctccaatacc cccttcattt gtttcagcat cattttgtcc
    67321 accacccggt ccggcaattt catcatcaat tcgatcatcc attttttcgc cacgatcacc
    67381 tggcgtgttt tgggtttgtc catcatgatg ctgttgtgga tcagcctggc cacttcctcg
    67441 gccggaatgg acatcttttg agaaaattcg atgaatttat ttttatgctt gtacagatcg
    67501 tgatagatcg tgccttcaaa agtatccgta tcgtatttgg ctttctcata catttcgctg
    67561 acaatagggc cgggttcgat gactatggca tcgatgccga aatccagcaa ttcgcggcgc
    67621 agggacccgg tgagtccttc caccgcatgt ttggaggcgt tataagggcc gtaaaaggga
    67681 cggctgaggc gtccacccac cgaactgata ttgatgattt tacccggctt cccggtctgc
    67741 ggtttcacgg cgcccaacag cggcaggaaa gcctgggtca cctggaccag tcctatgaca
    67801 ttcacttcaa gctgttccct gaacttgtcg atggacacat gctggaccgg tccggaaacc
    67861 gcgatgccgc tgttattgat cagcgccctg aggtattgac cgccgatcat ttcggccacc
    67921 tgtgccgcag cgacatcaat ggctctccgg tcacgcacat caaaaataag aggggtgaaa
    67981 cccggcccca tttctttttc cagttttacc gcatcttccg gtttccggac cgatccgaaa
    68041 acatggaaac caaatccttt gaggtatttg gcggtcgcca gcccgatacc ggtagaaacc
    68101 cccgtgatca ttaccgcatt catacgtgat tatttattgg gatgaataaa ccgttcaaaa
    68161 aactcccagg tgcgaagcat ctggtcgatc cgttgcctgt tgttcccgct gcgggtgagc
    68221 tcatgggttg ctcccgggtg acggacatac tccacggttt tacccattac tttcaaactt
    68281 ttataaaact gttcgctctg aatgaccccg gtacggagat cctgttctcc gtgaaaaatg
    68341 atgagtggtg tgcccacttg ctggacatag ttgatcgtgg attccctttc caagatatcc
    68401 cgggtggccg gttcccaggg ataccctcca aaatatcccg gcaccaggcg ccaggcattc
    68461 ccttccccaa aaaaggtctt caagtcataa acgcctcgtt gggaacaggc ggccttaaaa
    68521 cgatggtcat ggccgatgat ccaggcgacc aggtaacccg cataggatcc gcccgtgacc
    68581 accatcctgg aagtatcgat ccatccctgc gatgcggccc catccaacgc cttcaaaaca
    68641 tcatcggttg ggcctttccc ccagtccttg atgttggctc gcaggaattc ctgcccatag
    68701 ccccccgatc cccggggatt gcagtacacg aggccataac ctttgccgca ccagtattga
    68761 aattcgtgcc acatgctggc ttctccagga ccccacatgg ccgagggccc tccgtgaata
    68821 ttgagaacca ccgggtattg cttgcccgcg gcaaatccga ccggcttcat cacccaatat
    68881 tcgatgttca tgccctggtc gtttgtaaaa gtgaattttt ccggatagct gatatgtttg
    68941 tctttcagcc aggaggtatt gagggtggtc acgggtttct gatttttcag gctgcggtcc
    69001 gagataaaca attcataggg gctggacacc cgggtcttga caaaagcaag ctggttgtcc
    69061 cggctcacat caaaactacc tatcccctcg tcaaaaccgc tcagttgttc ttttttcaga
    69121 tcctccaggc gcaaccggta caagactacc ccgccattgg aaggcgaagt aaaatacaag
    69181 gaggtgccat ccggtgaaaa cttcaggttg ccgacagtcc gatcgtgcgg gatgaccttg
    69241 atatccctct ctttgccacg gaccggcata atagcgaggg cggggatacc gacacggttg
    69301 gtctcgcccc gggtaaaggc catccatttc ccatccggag aagcctgggc ctggccgaag
    69361 gacagcccgt cttttccaag caacttttgc aagttcccgt cctggcttag agtatagact
    69421 tcggattcca ggctgcggtc agggtgctcc ttttggtcga gatcggtttg taccagcaac
    69481 tggggggagt tgccaataaa tccggcattt gaaaaggaat aaaaaccgga gctgatggcc
    69541 cgggcttttg caccggcttt gacctcaatc cggaacaggt ggctgaagga gaggtcgttg
    69601 ctggtggtgg cctcttgttg aaataccagt ttgttgatga ccttggcttt tctatccttt
    69661 tcattgagcg ccaggtaagt cctgatctca tccaatgtgc cattggggtt ggctttggtc
    69721 ttggaaggaa ggatataggc attatcccgg aaacctgctt tttccatcgg ccattcggga
    69781 aaggaattgc cggcattgag cgtgctgtcc gcagccagat cggccagggt aaataaactg
    69841 ttgaaaacaa tatagctgcc gtccggtgac cagaccggat tagccgcccc gttcttttcc
    69901 cgggtcagtt gccaggcttc accaccttcc agcggaagaa tgaacacctg ggctttgcct
    69961 tcaatccccc gcacaaaagc aatatttttt ccatcggggc tccaggacgg actggaagca
    70021 ccgtcccagg tcaaggggcg cagtccattg ccttgggcat cccctatcca aagctgggtg
    70081 ccatattgat attcccaggg gtccctgggg tctggtttta tcgaagtgac ggtaaacagg
    70141 atccggtcgc cttccgggga ataggacaca ttggaaacag actggatgcg ggtgagatcg
    70201 gaaacctgga tttccggctg ggccatggca aaacaggtcc aaaaactcaa aatgagcggg
    70261 actaaaatct ttttcatcgg cttccttata aagaggaaaa atacaaaaat cgtcaatgaa
    70321 gaccggtatc ctttatccat ttcctgaatc caacggcatt ttgttttccg atgatcactt
    70381 cctgccttgc ggagggtttg agctccagca aaatcttgct ctgttcaatg gacttgaacc
    70441 ggacgatgaa gttccggttg accaggtatt gccggtttgc cctgaaaaaa agaaccgggt
    70501 cgagtctttc ttccagttca gtcagggtct ggtctgccac gtacaaatcc ccttcccccg
    70561 accgcacaaa acagatttta tgttcggtaa aagcataggc gatgcctgaa gcatccaggg
    70621 gtatcaaata tgcgcccttg tgaaccagga tgcgggttgc gttcactttc cggtctttga
    70681 gaaaatggtc catgccggac agaaaatgcg ttttgagctg acttaccttt tgaagcgcct
    70741 ttttcaactt ttcctccgtg accggcttga gcaggtaatg gacggcattg tggtcaaagg
    70801 ccttcagcag gtaatcatcg taggccgtga caaatatgac cgggaaaccg attttgcacc
    70861 ggtcaaagat ttcaaaggac agcgcatcgg aaaggcgtac atcaaaaaat gcaagatctg
    70921 ggggctcatg gtgccgaagc cagtcgacca ccgcttttac gctttcaggg gaagccacga
    70981 tttcgattga ggggtcggcc ttcagcagca agtgggccag cttgcgggaa gcggtgatct
    71041 cgtcttcggc caggatcact cgcatcagcc attgagtttc agaaggggca tacacaatat
    71101 gaaacggccg ccttcccgga aggacctgat gcttttgccc agcaacttgt caaacctttc
    71161 gtccaggttt ctaagcccgg tcgaggtgga aggatagcct tgtttccgca ccctcatttt
    71221 attgcttatt atgagttcgt cctccctgca gtcaatgtcc agcaccaggc gggaggtttt
    71281 ggaaagttca ttgtgtttta ccgcattctc cacggcgatc atcatggata cgggcggaat
    71341 gagataggtc tgtccctggg ccctggggtc aaacccgaaa tgtacctgga aggcatcctc
    71401 ataacggagt tccagcaggg atacgtagag gttcatgaag tcgatttcat cctggagcag
    71461 gaccacgtcc ttatccttgc tttgtaaaat gtaccggtac acctcggcca ggttttcggt
    71521 gtattggcgg gccttgtttg catcgttttc gatcagaaaa gaaaggctgt tgagggcatt
    71581 gaacataaag tggggatcga tttgattttt taaggcttcc agttcggcct ggatcttcga
    71641 tcgctggagt tgttcggcca ccagtttctc ctgttcagtc cgctttacca gcagcacttt
    71701 ttcatacatg ttggacacaa agattacgca gattgcaatg acggccaccg tgaggtaaat
    71761 ctgccggggg ggcaggtcat cggcaccggc cgcccatttc cagccaaaaa gaagaaggag
    71821 ggcagccgga atagtataaa acaaattgag cccgatcatg gtcaggtatt tctgaaggag
    71881 ggactggctt tgcagaatgg tggtgtgata ccggaacagg aggtagcggt tgccttccca
    71941 gaccatccag gcagtgaaaa caaaccataa ataataaatc aggataaggt ggaccggcag
    72001 ggctgcccgg tcgatcaggc cggtcgcact gggtatgacc aagccgaaaa aaggaacacc
    72061 gatcaacctc accacggtat catccaggat caagcgggca tcggaggatt tccacttatt
    72121 gctcattgtg cttccgtttc agcagccggt ttttccgctt gggtaaaagg agcccggcgg
    72181 gtaattcgcg gcttcctaga tttaaaccgt aaatcccaag ggcaggcaag cccatctttt
    72241 ttcattactt caaaaataaa aacaaattat tatggaaccc atccagatca atcacctcgc
    72301 agtcctggtc gctgccctgt ccaccttttt actcggaggc ttatggtggt ctcccgtgct
    72361 tttcgaaaaa gcctggaaag aagaaaaccg gttcaccgat gaaatgctcc ggcaaggcaa
    72421 tatggcaaag attttcgggc tcgccttcgt cttctcttta atcatgtcct acaacctggc
    72481 ttatttcctg ggggatccgg ggatcaaagc cggaaccggg gccttatatg gtttttttac
    72541 cggtttcggg tgggtaagca tggcgatatt tatcatcggg ctctttgaaa gaaaatcctg
    72601 gcggtatatg cttatcaacg ggggttacat ggtggtggct tttaccatca tgggattgat
    72661 cctcggggca tggaaataat gggtagaggc cgcgcttgcc ctcgacgatg ccaaaagatt
    72721 cggcaggatc ggattaaaag accggtagct cccttttgca agctatggga aatgcacgga
    72781 ctccgtttcc ttttatttaa ttttgcggct cgtttcaaca tgaataaagc tgcacttctt
    72841 ttctttttgc ttttgtccgt ccgttttcct gcaggggccc agaccccgtt ttccggggac
    72901 ccggaccaat ccctttttct taccacggac attgaccatt tttggacggc ttacgatctt
    72961 tttgtaaaag atacctcggt caatcctttt ggaccccatt acctcgaggt gggaagcaag
    73021 ggcgtgaaag gattcacccc cttccggatc cggagtgcgg actatctcta caacaccgtc
    73081 aaacggcaca gggcggatta tgatagcata aggcaaaaca ccctgcgcat ccatgaaaca
    73141 gaaaaacagt gcaggagcgc tttttatgcg ctgaaatact ggtaccccga agccgtttat
    73201 cctccggtat attttgtgat cggtaccttc aattccggag gaacctttaa caaaaacggt
    73261 ttatttatcg gagcggaaat gcaaaaagag attggcaata tcccccacat cgtagcccat
    73321 gaactgatcc actttcagca aaaaaactgg gtcaatcaac ccaacctgct gaggcaaagc
    73381 ctggttgaag gcagcgccga ttttatagga gaactcattt cagggaagca tatcaaccct
    73441 gctgctcatg cctacgggga ccgccacctg gaaaacctgt gcagggaatt cgtggcccgg
    73501 atggacagta cggattacca ggactggctt tatggggtat cgggaaaaga cgacaggccc
    73561 aatgacctcg gatactggat gggctataag atcacccagc aatattttaa taaatccgcg
    73621 gacaaacccc aagcgatccg ggacatcctg gatatcaaag accctttgaa attcctgcgg
    73681 gcgagcgggt ttctggatcc ctatctggag tgattcagcg gggaaaacag ttgaacaagg
    73741 aaggagcgcc tggcgcattc cgtggttacc atgaaaaaaa ctaaggttta aggtgtccgg
    73801 aaaaaccgga ccgggcagct tttaaaaagc ggagcctacc gggcataccg ccgatactta
    73861 ttgtttataa ctttcgtaaa tgccgttcag caacttcttc attttatcgt aggaaggaag
    73921 gccgtacttg tttttcttgg gtttgtggta tacgttttca ttcatccatt catcgtcata
    73981 cagcttgctg aggtataagc ttccgtgatc ggggaccagg atcaaaatct gatcggtggg
    74041 tttgaggtct tttcggatct caaacacccc ctggatcgcg gccccgccgc tgggcccgat
    74101 catcaggcct tcttcagccg tcagtttaat ggcccttttg atggctagct ggtctttgac
    74161 ctgaaccatc tgatccaggt gttcggtgat aaaggtcatg ggaatgatgt tcttacccac
    74221 tccctcgagg gcatagggct tgatgatcga ttcatcgaaa atgccttctt caaaatattt
    74281 tttaagcacg gagccatagg cgtcgatgcc tatgactttt atattcgggt ttctttcttt
    74341 aagatatctt gaaatgccgc tcagggttcc cccgctgccc gaacaagcga ggaaatgagt
    74401 gatggccccg ttggtctggg tccagatttc aggcgccgtc aggtgataat gggcatccac
    74461 attatccata ttgtaatact ggttgacaaa ataggagttg gggatgctgt cggcaaaaaa
    74521 ctgggcctgg ctgtaataag accgcgggtc ttccggcttg actgaagccg ggcagacatg
    74581 tacttccgct cccagggcaa ccaggatcct ctttttctcc tcactgattt tgttgggaac
    74641 cgtgaaaatg catttatatc cccggtgtcc gccgaccatg gcaagcgcgc ggccggtatt
    74701 acccgaggtg cattctataa tcgttcctcc gggccgcagt ttgccttcct tttccgcctt
    74761 gtcaatgata tacagggcga tccggtcttt ggccgaatgc ccgggattat aagattcgaa
    74821 tttggcgtaa acatttgaac ctacctgttc tgagatcgtt ttaagcttga tgatcggcgt
    74881 gttccctatg gtctcgtcta cagagttgaa ataactgaat ggcataggca aaaaaggttt
    74941 tggtatatgg cctgagggtc aacaaagata aaattatatt taaattttaa agtctgattc
    75001 tggatggtgc cgcccatgct gtgcagcata aaaaaaccgt tatccggcag aaccggataa
    75061 cggtttatat attatggatt aatttaaaac gttagcgctt cgcggctgtc gttttaggtc
    75121 ttggctttgt ctgtgcactc accccgggcc attctttcca ggcattctgc ttttgacatt
    75181 ttactgcaat ccattttagc tttttgcccg gattcattgg aaactctcag gagctgggcc
    75241 tccgaagaaa ccgccggagg ctgattgaca aatgcgttgg atttggcatc aaagttcagc
    75301 gcggcataag aaacatgccc tccgtaagaa cacatttttt tacggaaata cgatacctcg
    75361 ccggtggcca catctttacg aacttcaatg gaaggatcct gcgaagcaac cagggaagcg
    75421 gccgctgcgg aagatttttt gcaacaacct gacatcgcgg atttggaatg acaggattgt
    75481 ccgtacaatg cccccgtagt caacagcagg gaacaaacca aagtcatcag caattgtttc
    75541 atagacaata tcttttgagg tgattaaaga tttcaaaaat aagtccattc agggataaaa
    75601 gcaatacctt cgatttgaca tataataacc agttaccgtt tcgttttacc atcaaataag
    75661 gaaggccgta ttgacaaccc gcatcttcat attgaaaata ttaatgaccg cattgctcat
    75721 ggcccttttg cccggaaccc atactgccca gaaattcaaa cctgaaaaat ataaaaaggc
    75781 cattctctat tttggacacg gcgggggctt tgcaggcgct gaaaccacct atgcgctgat
    75841 ggagaatgga cggctgtact ccaaaaaaag ccttacggcc aaaaccttta cctctttaat
    75901 caagctggaa aagaatcaat cggcccagat tttcaataat tatttcttcc tggggattcc
    75961 ggaaatgcaa ctggaagatc ccggcaatat ttacaaattc gttgaatata cgcggaaaca
    76021 aaaccggcac cgtctggtat ggggtggcca atcccctgta ccgacaaacc ttgttttatt
    76081 ctattccaac cttatcggcc ttctgcccaa accataatc
//
LOCUS       DAOQXT010000006        75083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_341_length_75083_cov_8.640494, whole genome shotgun sequence.
ACCESSION   DAOQXT010000006 DAOQXT010000000
VERSION     DAOQXT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 75083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75083
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_341_length_75083_cov_8.640494"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..956)
                     /locus_tag="PKM27_01470"
     CDS             complement(351..956)
                     /locus_tag="PKM27_01470"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR05950.1"
                     /translation="MKKTIAFLFSILFGQLAFTQSLTRDERKEALEFIDWTTKELKKS
                     LKGLSAAQLNFHPAEDAWSPLECLYHIAYSEKALRATLDAALQAPADPATKARLTTTD
                     LQIKNNIMDRSSKFKTAAPFEPLNTGFKSFEEAWTVFEERREALAELIKNSDLDFRNH
                     IVTRAFGKMDAYQFVIFIAGHSNRHTQQIEEAKGIAGYPKR"
     gene            complement(1155..3143)
                     /locus_tag="PKM27_01475"
     CDS             complement(1155..3143)
                     /locus_tag="PKM27_01475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNR05951.1"
                     /translation="MKKTVLSVFLLSCGYYLLAQSAWTAEKCLELKNITAVTASPDGS
                     KVLYAVREAVMTADRSEYINQIWMTDIKTGKQMPLTRGDKNSAQPAWSPDGQWISFVS
                     NRDGKNNLYLLPVAGGEAEKITDVKSGIGNYKWSPDGRSLACVIADPVTGEDEKNTKG
                     KEDWYYMDEKIKQNRLYVLKLDQKDSAGARVMKKLTEQDLNVNAFDWSPDGKQIIFSH
                     GKSPEVNDNVYSDISLINLEDGKIKSIANSGAGESNPLFSPDGKYLAYASTTDPVDWS
                     GPEHIRIYSLADGKTWKLKATPDENASLIGWTADSRSLLVNESNKTMSSVYILSVDGS
                     SAKEWNAGSRMHLSAANLNKTGGHLAFVLQNASTLPQVYLSTIHEYKPILVSNINEAW
                     KSKTLPRTEVIRWKSKDGKEIEGLLTYPVNYQAGQKVPFILNVHGGPAGVFQQTCIAS
                     NQAVYPLAGLAEKGFAILRPNPRGSSGYGADFRMANRKDWGGMDYEDLMDGVDEVIRI
                     GVADTSRMGVMGWSYGGFMSSWIVGHTNRFKAASIGAPVVDLAHQNLTDDIEGFLPSY
                     FKADPWNDWSLYDKHSPLRFVQNVNTPVLLQHGEADKRVPFSNAVMFYNALRRREVPV
                     RLLALPRQPHGPQEPKMVLKTLQSNAEWFDEYLMNKKS"
     gene            complement(3260..5686)
                     /locus_tag="PKM27_01480"
     CDS             complement(3260..5686)
                     /locus_tag="PKM27_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNR05952.1"
                     /translation="MKLKQAASFFSLLFLFIPFGIKGQTAKNAQVKGRIVDSLSGQPL
                     GFASIQVLSVMDSSLVNGDLSTDDGSFAISAGYGEFFLRLDFMGFRGKTTSSFILSKE
                     HSRHDLGTIQLAPLVSTLEEVVVQAEKSSMQLALDKKIFNVGKDLANAGGSASDILTN
                     IPSVSVDAEGGVKLRGSDNVRILIDGKPSGLVSIKGGSGLQQLQANLVERVEIITNPS
                     ARYEAEGMAGIINIVLKKDRSQGFNGSVDLITGNPANFGLAANFNYRRNKLNFFINYG
                     ISYRDQLIRSSLYQEVYGDTTRLLEQTNRGTFTGFNNNVRGGLDYFFSEKSILTAAYL
                     YRRSQGNRLLNIQYQDYLENLGGSPNLSKRQQDEDEIEPNSEFSINYKRAFDQKGHEL
                     IAEVKYIDYWENSDQTYGQQFFLSDGTEDLSRNLVQKSVNDEFEKQYLLQLDYVRPIG
                     KEGKMETGIRSSFRDMVNDYVVSQKNETGTFIPLPGLDNIFLYDENIHAAYGILGNKS
                     GRFSYQAGLRAEWTDVKTTLQETNQVNPRDYVNLFPSVHLTLSLPRENALQWSYSRRV
                     RRPFYNDLSPYVTFSDARNFFSGNPDLDPEFSNALELGHIKTFEKGSLTSSVYFRNTR
                     DRIERIRLVDESGNSVTRTENLLSERALGVEFTSGYSPLPWWKLDFNFNYFYADIDGT
                     NILPDYRAYTYSWFTRQTSRFTLPRGLDIQLRANYEAPQRTAQGKRKALYFADLSVSK
                     DLWKDKGTLNLSVLDVFNSRRMRIVSEGPNFFTEGNIRPRVRQVNLSLSYRIKQAKPM
                     KPRRMEGEEG"
     gene            6244..7086
                     /locus_tag="PKM27_01485"
     CDS             6244..7086
                     /locus_tag="PKM27_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNR05953.1"
                     /translation="MKKLIFFFIVLVLAADTNAQKPLYTAPFGIQAFTFRRSFPNSIE
                     KTLDAIAMMGFTEIEGSGGRIAPEDFRKLCEARGISIPSTGTGYEQLVKNPMEVAARA
                     KALGAKYVMCAWIPHKNGAFNFENAKKAVEDFNAAGKVLKEQGITFCYHVHGYEFWPY
                     EKGTLMDYIIKNTNPEYVSFEMDVLWTQFGGGDPVKLLKKYKDRWKLIHLKDLKKGVK
                     KDLTGGTSTENDVALGTGEIDIPGIIRQANKIGIKHFFIEDESNNILSQVPQSVAYLK
                     SLKK"
     gene            7176..8111
                     /locus_tag="PKM27_01490"
     CDS             7176..8111
                     /locus_tag="PKM27_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05954.1"
                     /translation="MTPFKLFYHSACILWMVCISSSCAARGDSPDLILVGSTPGDDLI
                     KSLLAIPPETKIDFIKWNLALSPSNTGQETFVLGMVVGESQPNTLGFIGGGKPLTIKG
                     TYRVSKEMGGHRGAETVRLTSESLPSGISLAKLNDNLFHLLTPEGKLMIGNGGWSYSL
                     NRKYPVPLTGPSAFSNPFKPAGEPSFEVVFDGRTPCREIAAEHPEMNASPSCFKLKWR
                     LILHRDSANHLPTTYTIRKVVDNEPRDVTGTWTILKGIPSNPDAVIYLLDPDKPAESI
                     SFLAGDENVLFFLNRNHEPYIGNEDFSFTMNRRIQ"
     gene            8225..9331
                     /locus_tag="PKM27_01495"
     CDS             8225..9331
                     /locus_tag="PKM27_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05955.1"
                     /translation="MKTIPFLSVLILAHILCGSGPAKAQIELRVKPNEIRSTVSPQMW
                     GIFFEDINFAADGGLYAELIKNGSFQFPLPFTGWKVNKVDFKDKVLVVNQSSVKNGNT
                     RYVRIAKSSDSSVYNIVNEGFRGIGLHKNAGYTFSMRARTISGNLRLRVEVLRESGQS
                     LVRGDIANLKGGWTDYTLPLTAPDSESKGLLKIAFLGTGVVEIDLISLFPVNTYKNRP
                     GGLRADLAAMLDSLKPGFVRFPGGCIVEGHTLASRYQWKKRWDQNRSGNCPSTGGIWN
                     SQEGRHRTIISPSAWVFMNISSCRKIWAPNLSPSSIAAWPASSTAARSLPWMTWGLIS
                     RMPWTLLNLPMALYKPNGANSVRTWDIRLPSTSR"
     gene            9328..10209
                     /locus_tag="PKM27_01500"
     CDS             9328..10209
                     /locus_tag="PKM27_01500"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNR05956.1"
                     /translation="MIGIGNEQWDEQYFERYQKFEQALKAKYPAIQLVSGSGPSAQGN
                     LFDYAWSQFKDSKAAFVDEHYYLPPAWFFANARRYDHYDRNRPKVFAGEYAAHDKEDA
                     QPESRNTWLSALAEAAFMTGLERNADLVRMCSYAPLLAHVEAWQWRPDLIWFDNLRVM
                     ATPNYYVQKLFSTNRGSHMIPITRQDSVIAGQDSLYASAVLDKNQNQVIIKLVNAARK
                     AQKINILIDGLPPDKFQARGTVLSSNADLAFNRMNSPSVITPRETSYTIESGVLTEDF
                     PARSVQVIVLQYGSIRR"
     gene            10509..11519
                     /locus_tag="PKM27_01505"
     CDS             10509..11519
                     /locus_tag="PKM27_01505"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HNR05957.1"
                     /translation="MKVLNCTTPGHFEYQDKPEPASRAGYSLLRIQKVGICGTDLHAY
                     KGRQPYFEYPRILGHEVAAEYVSGDAPDIRPGDLCTFLPYISCGSCIACRSGKTNCCS
                     RLVVCGVHLDGAMAEYYLVPSPLIIPGENLSEDELALVEPMSIAAHAIRRAENIKGKQ
                     VLIFGAGPIGMCTSILALQAGAVVGLADVHAGRLAFASSFYSIHTFLNRDPSHFINEV
                     RNWTNGDMAPVVIDATGNLQAIESGLQYLAHGGQFILVGLQKEAFHFSHPEFHKREAT
                     LKSSRNATRKDFIRVRDLIKNKTLSVDPLITHRIPFGVVAKQFETLYDPGNLVMKAVI
                     EF"
     gene            11665..12762
                     /locus_tag="PKM27_01510"
     CDS             11665..12762
                     /locus_tag="PKM27_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNR05958.1"
                     /translation="MIVLYFILLAPFLSGFFQENQPVEKIQIDGPYVVYKNDLIYVQY
                     IYQEGGIKSVTTDSFPVSQKKNIELRVATDEPGKYFTVKLKNRLSNEKSEFGKPAKLL
                     AISDIEANFKALRTLLQVNGVIDQEFNWTYGKGEVVFTGDFFDRGSQQNEVLWLMYAL
                     EEKAREAKGYVHFVLGNHEIMNMNGDFRYVHPRYMEHAKMMGVPYISFYWDNTELGRW
                     LRTKNIAQRIGDILFVHGGISTPVNYMNVPAPAINELARPFYPDTTYNYPDIWTEILF
                     SDLGPFWYRGYYSKKSPATSGQVDTTLNVFGVKYITTGHTVVADTISVLYNGRVFNTD
                     VYHAGGNSEAMLVEGGKFYRVNSKGEKFLMK"
     gene            12843..15698
                     /locus_tag="PKM27_01515"
     CDS             12843..15698
                     /locus_tag="PKM27_01515"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNR05959.1"
                     /translation="MLSILSANKEVRGLSILLLFCFPFLAKSQIQWPEIGRDTRPWTR
                     WWWQGSAVDTPGLKWAMKQYQEAGLGGLEITPIYGVQGHEARFIDYLSPQWMGVFDFV
                     LREAKKLGLGIDMATGTGWPFGGPWIGEDQACKYLAHKKYQLREGQSLKDTIQYFQEG
                     FVRTANGERLSVADVIYPITDNEDLQALAIDQVRHPVALPLIGLFAFNDLGQWMDLSQ
                     KVNKGLLEWTAPQGTWTLYALFQGLHGKMVERAAPGGEGNVIDHFDEAAVREYLSFFD
                     RAFENTDLSGLRGFFNDSYEVDDARGQSDWTPRFFEEFNSRRGYDLKAHLPSLFSETP
                     DEKSALVLYDYRETVSELVLEKFTRTWTHWGHQKNKWIRNQSHGSPANILDLYGAVDI
                     PETEGTDLLRFKFASSAAHVLGKPLASAEAATWLHEHFISSLSDVRAAADQYFLGGVN
                     HLVYHGTNYSPPNESWPGWLFYAAVHLNPANPAWKDFKALNDYISRCQAFLQKGNPDQ
                     DILLYFPFADRNQQLGNSVLHHFDGMQGFEGSIFKSAAEELIQSGYAWDLISDRQIQS
                     LQCLNGKLIAPGGDYQTMVISGAEFMPIQTLFKIDSLIRKGAKIIFHVYPTHVPGFYL
                     HEENQRLLDSLVNALQLKKNQAGKLYMDHGLGRVYKGPELQALLDAAEIRSEKAIYQK
                     NLACIRRKTETGYYYFIKNTGDSELKDWLTVNASAASVLQFDPMKNQSGLARWKKTAS
                     GTEVWISLLPGETVILETTRQKIEGPSFPFFEPAGEAVDQDSGWNLKFISGGPALSES
                     VRIDQLQDWTKLPLPGAPTFSGTASYSITFKLPSKRYDHYLLDLGTVFETAEIKLNDS
                     VLATTIGPVHRVLFPSSLLKRTNTLEILVTNSMANRIIDLEKRGVPWKKFYNINFPAR
                     LAENRGPDGLFTAIHWDPRPSGLLGPVTLKPFNKAKE"
     gene            15762..16166
                     /locus_tag="PKM27_01520"
     CDS             15762..16166
                     /locus_tag="PKM27_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR05960.1"
                     /translation="MQLNKIQHIGIPVTDLGKSVPFYQRLGFEAAMESPFDYQGERGT
                     CVMMKQGEILIELYQFPARFLPEIRNRKDGPVDHIAFDVQDIDLVFSGMKARGFNPIE
                     ESPVFLPFWKNGCRYFYILGPDGERLEFNQIL"
     gene            16228..17001
                     /locus_tag="PKM27_01525"
     CDS             16228..17001
                     /locus_tag="PKM27_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNR05961.1"
                     /translation="MEKWYPFWRISVLSLVVSSCVYDSAVIPTDCTNIHWTYTMTAGG
                     QDHWADLCTGYNACGGMSQSPVNITGAVAQPNLSKLTFGYSTTAAEIENNGHTIEFVC
                     DPGNKLTIGGTQYELLQFHYHAQSEHQVDGKKSPLEVHFVHKATDSDFAVIGVLFEEG
                     ASNSLFNQYLSRFPAEEGTYTDHSEIELTELLPLNKSYYHYSGSLTTPPCSEVVHWYV
                     LKNKITASASQLAQFQALLKNNYRNIQNLNGRKIYSFDE"
     gene            17029..17367
                     /locus_tag="PKM27_01530"
     CDS             17029..17367
                     /locus_tag="PKM27_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05962.1"
                     /translation="MELSKKDKKTCRELIETGLQREFTTGLEKAESILCNWKAKNKDV
                     KETYHALYSMIRKFDNHIGRRYNGITGSHYLSCVAGLVIDQIITLEELSPFSDEARAT
                     ILSMSSIKWL"
     gene            17379..18656
                     /locus_tag="PKM27_01535"
     CDS             17379..18656
                     /locus_tag="PKM27_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNR05963.1"
                     /translation="MFSFSFFKLFFRPESGMAVFLTALLNWPLSVQAQYPGIADDINR
                     HRKGVLKVRANPGDMVTVEQLRHDFWFGCALSNGFGGGTMKEEDHKQYEEKFLQNFNS
                     AVTENAVKWPSMEPRRSEVNYKVVDQLLQWTEKHQIPLRAHNLFWGIPRFVQDWVKEL
                     DDQSLLQTLENRAETVTRRYKGRFAEYDLNNEMIHGNYYEDRLGPDITRLMAQWAHQG
                     DPEAKLYLNDYDILTGNKLAEYLSQIRKLLAQGAPIAGIGVQGHLHSDTFDRNQLKNA
                     LDSLGLFGLPVRITEFNMPGQRSKYHIEKITEMSPEEEALRAREMVDYYKICFAHPAV
                     EGILMWGFWAGANWIPSSSLYKRDWSPTPAAQAYQDLIYKEWWTKESGTAGSEGLFSV
                     PAFYGKYKVTVGGLSKEIHLTKGKGQIVVDFKK"
     gene            18681..19211
                     /locus_tag="PKM27_01540"
     CDS             18681..19211
                     /locus_tag="PKM27_01540"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR05964.1"
                     /translation="MSDLKKIEKPEPGEYPFYASMYIDLLPDDGLLLKHLQENLNLVK
                     QMVSAIPGEKWTYRYAPEKWTIKESLVHIMDDERIYAYRALCFARNEKIPLPGFDQDA
                     YVLLSNANERSIDNIMEEYEAVRLATIALFNGFDEAALMRMGTANKNNATVRALGYHI
                     AGHELHHINIIKEKYL"
     gene            complement(19307..21469)
                     /locus_tag="PKM27_01545"
     CDS             complement(19307..21469)
                     /locus_tag="PKM27_01545"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR05965.1"
                     /translation="MFSKKIIPLLFSVFYIQVACAQVPGQGIIENRYDQLIEAPQVAD
                     QWENWRREISDWKDSVLHRMAYRGEIYRKPEYQWASKAYSTLFLMANDKNLYDPYGNF
                     AIKKYLEKYEEGYGGVDVVILWPTYPQLGFDERDQYFFYRNLPGGVKALKALCEELHA
                     MGKKLMIAYNPWDHTARLAGRNDVDELLSLLKEIGADGFFLDTISNFESLFQVLKTSS
                     PGAVFQSEIPIVPEALDQVHQSWLEMAWTRNHALEFNEVPTLVRNRWLEPRHMIYRLS
                     RHSHEQSTLIQNAWINGCGIVIWENVFGTVNELNPRDRSLLDAMLPAQRRYSRFFTEG
                     RWTPLVPVWLNRVYASQWELEGKKLWTLINRQEQWAKGTVLADDFIPGMKYYDLITGK
                     EVKTKTAGGKTFLIIELRPKAISGILSIPENERTADFSNFLQSQASKDKNSDFSTAYS
                     IPGHHLKPVPNTVKYLKNKLPQGMVEIPVSRDPVILEFQFRQRECGFYPLVGIEDFSY
                     SRAINQILSAPVKGMLNSYAMDETTVTNAQFKRFLEDSGYKPAHPENFLKHWKGNKIP
                     EGLEAHPVVWVSLDDARAYARWARKRLPTEAEWQWAAQNGARETTYPWGNEFDSTVCN
                     HGQVGGLSPVKQYEKGRTQSGLYDMSGNVWQWTESERTDGYNNYCILRGGAGYVNRNS
                     HWYADQGAQKTKFGAKYLLTYPGLDRCATVGFRCVVDL"
     gene            21622..22941
                     /locus_tag="PKM27_01550"
     CDS             21622..22941
                     /locus_tag="PKM27_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR05966.1"
                     /translation="MKKNKISGNDRRTFIKSTGAAIMGGTVAFNLGLPGQAFARKTFK
                     TANTLKVGLIGCGGRGTGAAAQALAADPDVVLTAMGDVFLDRLEESYKSLVEAAAQKV
                     KVDPSHKFVGFDAYKKVIDSGVDVVILTTPPAFRPDHLIAAIDAGKHVFCEKPVAVDA
                     PGIRKVLAAAKKASDKKLSLVSGFTFRYEYAKRAFFERITQGEIGEVRTVSSTRNGGY
                     LWYKPRQENWTDMEYQMRNWYYQNWLSGDYLVEMIVHSLDMMSWAVGDKLPLQATGTG
                     GRQARVDKMYGNIYDHFAIEYEYGNGIRGFSFSRQQAGCSTRNSVEIAGSLGHAVVKS
                     TGIPEINGKTRWTYQGEKNDPYQTQHNELFASIRNGKPMNDGQMMANSTMVAILGRMV
                     AYSGQTISWEDALQSNQVLGPSIDQFNWDLKFAGPEVAIPGITKVLG"
     gene            22969..23709
                     /locus_tag="PKM27_01555"
     CDS             22969..23709
                     /locus_tag="PKM27_01555"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR05967.1"
                     /translation="MARFIKPCCWLFLVSNFQYSVAQPSTLSFGKEVSSIQEKRKGTT
                     INWINVNTDPGTWTLKKNELVCSGRPIGVMRSEKQYENFILHVEWKHMEPGGNSGIFV
                     WSDAIPGEKNRLPNGVEVQMLELDWVKLHTKDGVEPPIAYVHGELFGVGGVETVPDNP
                     RNTRSKSIENRCRGRGEWNTYDVVCVDGVVKLSVNGKFVNGLAKATQKKGYLCLESEG
                     AEIHFRNFYLMELPPGVTSASQAAPEIN"
     gene            complement(23750..24238)
                     /locus_tag="PKM27_01560"
     CDS             complement(23750..24238)
                     /locus_tag="PKM27_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNR05968.1"
                     /translation="MKQENLILEPKPLLTRSWSGLAASLLLGIGLFAAGYFTGKPDGS
                     RDSMAMPAPKASKFILLVHNDDLPAQDPMQQVREYRAWLEDIQAKRIAGGEHLHSSGW
                     ILSGKNRENRVEARPEFMGRQELGGYFTFEADNADEALAIAKTCPHLNYQGTLELREV
                     YR"
     gene            complement(24347..24862)
                     /locus_tag="PKM27_01565"
     CDS             complement(24347..24862)
                     /locus_tag="PKM27_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNR05969.1"
                     /translation="MNFIDQALVTTYLRTRDETAFRLLYSRHAQALWNMAMKLAAGNN
                     SQAEEMVQDTWLRTIIGLSSFEWKSSLRTWMIKILINRWREMNRKKSEVPLEESHMQS
                     HAHTIKINDPQPLMALLPEGYRTVLLLHDLEGYKHEEIARMLDIAPGTSKSQLHQARR
                     AFRNLIQKENI"
     gene            complement(24983..25822)
                     /locus_tag="PKM27_01570"
     CDS             complement(24983..25822)
                     /locus_tag="PKM27_01570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR05970.1"
                     /translation="MMACRPMTEADIPRGLDLCRQAGWNQLERDWQIFLKLSPGACLA
                     MEEGKVIGTAVTLPYGDRFSWIGMVLVDREYRGRGIGMELLRKALDLINKETTAKLDA
                     TPEGRQLYLKMGFVDEYPLSRMIKPGVNHSPYISAAKVFQKNEWGAIKEADSPIFGAD
                     RLSLLEWIWTWAPEMAFVLKKNNKLLGYCFGRPGHRYFHIGPVVAADAETARHLVLAA
                     INQGRGRSLLLDVPHFDPSWPVWLKSIGFEEQRPFYRMYRGSNRFPGEPEKQYAILGP
                     EFG"
     gene            complement(25819..27015)
                     /locus_tag="PKM27_01575"
     CDS             complement(25819..27015)
                     /locus_tag="PKM27_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR05971.1"
                     /translation="MKEDKLSPGHFMVGATGFLSLFSLVGIMFYGLPFFYDFWVQEFG
                     WTRATVTSGNAFAKVFVGLLGFFAGWFIDRYGPRRLMLAGILMGGIAVIGLSQMTQLW
                     HFYVFYLFNALGYMCGGPLPNQVLISRWFSKSRGKAMGIAYLGIGIGGMLVPQAARWL
                     NVHFGWHLALMILGMAMIAIAFPMAWFVKENPEVNLQEKKTETEKIHLAGVLKSWPFY
                     LLVIGSMCSIGAVSGTSQNLKLFFSLDLKYSQGEAANIISLILASSIFGRLFMGWLAD
                     RIPKKYVMVLIYCLVAGSIPLLYSVSTPGVVYLFAILFGIGLGGDYMIIPLMAAELFG
                     VRVMGRVMGLILTMDGLGEAFAPMLVGWLRDISGSYANGFAALIALAAIGALAIAFLP
                     KKMNKA"
     gene            complement(27037..28314)
                     /locus_tag="PKM27_01580"
     CDS             complement(27037..28314)
                     /locus_tag="PKM27_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNR05972.1"
                     /translation="MKNKRRDFIKYSGLAGMGLGLKGWRDLESFDRLNAVYPGFNEWQ
                     NESLSIIGQYGPWAAKRMEGKRPSFSFRNPEWTDPEAWRKSARQRLKERMAIPELGST
                     PRVTVHKQYVYDGLYIEELSWPLPYGRPTEAVLLKPANAKGPLPGILAFHDHGGNKYF
                     GTRKITKTSDQQHPLMVSHQRDYYSGFAWANEIAKKGYTVLVPDAFVFGSRRVMMHDV
                     PVSQRRGLSDEDPENPEKIKAYNEWAGQHEHILAKSLFCAGTTWPGVFFAEDRVALDV
                     LAARSDVDANRLGCGGLSGGGLRTVFMAGLDERIRCAVCVGFMSTWKDFLLNKSYTHT
                     WMTYVPLLPNELEFPEILGLRAPLPTLVLNDVDDALYTMDEMKKAEQILKEVYGKAEA
                     ARQFECSYYPGPHKFDAAMQKEAFVWFDKHLKS"
     gene            complement(28350..29363)
                     /locus_tag="PKM27_01585"
     CDS             complement(28350..29363)
                     /locus_tag="PKM27_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05973.1"
                     /translation="MKEEVFGLLHEGTVIPAHPLALDSRLKLDEARQRGLTRYYMEAG
                     AGGIAVGVHTTQFEIRNPGIGLLEPVLRLAAEEVDQAALNRPFIKVAGICGPVEQAIK
                     EAELAKRHGYHFGLLSPGGLAAYSENELLERAKAVSEVLPVFGFYLQPAVGGRVLSYD
                     FWLAFSEIPKVHAIKVAAFNRYQTLDVVRAVCESSRKDQIALYTGNDDNIVADLLTPY
                     QFMVSGRQVTKRFVGGLLGHWAVWTGKAVELLKEIKTCIGQGYAGTERLLAKGIAVTD
                     INAVVFDAKNNFKGCIPGIHEILRRQGLLEGVHCLNPEETLSPGQGEEIERIIRMYPD
                     FRD"
     gene            complement(29397..30413)
                     /locus_tag="PKM27_01590"
     CDS             complement(29397..30413)
                     /locus_tag="PKM27_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR05974.1"
                     /translation="MEKIGEIYQKLLRPSSELIADISSLDGDILILGAGGKMGPALAR
                     KAKQAIDAAGINKKVIGVARFSEAGLEKELNREGIETLRADLLRDDQLQALPTVKNVL
                     YLAGTKFGTQGNESFTWAMNAYLPGRVAEKFKDSRIVVFSTGNVYPFVSVKSGGAHES
                     IQPEPLGDYGQSCLGRERLFQYFSILHRTPILIYRLNYANDVSYGVLLEIGKSVKSRK
                     PIDLNMGHVNVIWQGDANEIALRALPHCAVPCKLLNVTGPETASVRWIANEFGSLFGE
                     PPIFTGEEQDTALLSNAAECFRLFGYPRVGLKQMIGLLARWIEEGGKTINKETHFQER
                     KGKF"
     gene            30642..30908
                     /locus_tag="PKM27_01595"
     CDS             30642..30908
                     /locus_tag="PKM27_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HNR05975.1"
                     /translation="MQWIDYVGLFGAFLSSVTFVPQVYKAWQTRSTGDLSYWMLLILL
                     GNVSTWLFYGIVKKDLAIIIANAIILSLALILLYFKITFKSKPS"
     gene            30941..31549
                     /locus_tag="PKM27_01600"
     CDS             30941..31549
                     /locus_tag="PKM27_01600"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNR05976.1"
                     /translation="MPSASYPPSPVTLTGEQVSLIPLGETHENDLIEATRDGELWRLW
                     YTSVPSPETVKSYIETALNEQALGKSLPFAVLRHADKKIVGTTRYMNIEPGIRRLEIG
                     STWYATHAQRTAVNTECKYLLLQYAFESLECIAVEFRTHRFNEASRRAIQRLGAQQDG
                     ILRNHRLQPDGTLRDTVVYSILIQEWPVVKSHLRFKLAGGGR"
     gene            31697..32863
                     /locus_tag="PKM27_01605"
     CDS             31697..32863
                     /locus_tag="PKM27_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR05977.1"
                     /translation="MESLIDRQILPEARKALRQFRALCITGPRQSGKTTLSKQLFKGK
                     TYINFENPSTQFEAELNPDRFLKQYLSGAILDEVQRVPHIFRYLQEILDNNPSRGQFI
                     LTGSNNFLLHEQVSQSLAGRAGYLSLLPFSYAELKASNLAYSDVHRLILTGGYPEIWQ
                     EGLNPGQWLNSYVQTYVQRDVRLIRNISNLAVFTRFLHLCANHAGQILNREELARQTG
                     VDTKTILAWLGLLENSYILFLLQPWYQNLNKRIVKSPKLYFYDTGLLCHLLGIKNVQA
                     LTRHPACGALFENWIITEIRKNTFNTGSQQGMYFFRDNVGNEVDLILDREEGPLAIEI
                     KSSTKAEGAMLRNLRFWLKNTRDGRALLIYRGKKNEFTEDYIGIAPWTEVSDLS"
     gene            complement(32902..34302)
                     /locus_tag="PKM27_01610"
     CDS             complement(32902..34302)
                     /locus_tag="PKM27_01610"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HNR05978.1"
                     /translation="MYQDLKEKLKTLPPEYPRPLLPAIRSEFLKSGKTILVLDDDPTG
                     TQTSSEATVITSWRPEEIVAELDKKPSVLFILTNSRSLPEEDAIKLSQDIGRNLNIAI
                     QKSGREIVLISRSDSTLRGHFPAETDALVEAMGLQQAIRVLVPAFIEGGRYTIEEVHY
                     LAENQQLVPVSETAFARDRVFGYKHSNLKNWVEEKSKGKVRAAEVLSVSLEDIRTGGP
                     EKVAGIFMDCREGKTVIINAAGYRDLEVVALASILAGQNGKTILFRSSATFVPIRAGM
                     ESGRPFKPSPLQQAGGSGTLVVAGSYVSKTSQQLNALLARNTHKAIEVDVVKVLKGLE
                     KPEPITGQINQWLAADKKVLVYTSRLLQEGHDDISNLHINAQVSDFLVSLLKGLAVRP
                     RYILAKGGITSSDIASKGLLVKSARILGPVIPGVPVWQLGPESRFPGMYYIVFPGNVG
                     DERSLETVCQIMEGIK"
     gene            34393..35346
                     /locus_tag="PKM27_01615"
     CDS             34393..35346
                     /locus_tag="PKM27_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR05979.1"
                     /translation="MQIIITGGAGFLGQRLAKAFTDGNFDFKSLQLVDQVMPANPAND
                     HRIECRQADLTEDGVIKTILKPDTSLLFHLAAVVSSHAERDFDLGWKVNLDLTRQLLE
                     ACRHHNPSIRFVFASSLAVYGGLLPETVGDTTALTPLSSYGSQKAMGELLVNDYSRKK
                     FVDGRVLRLPTVCIRPGRPNQAASSFVSSIIREPLNGEEALCPVSPELALWLSSPDTV
                     IQNIIHGALLDASAFGAWRSVNLPGLRVTVKDMLDALGKVAGRNTLDKIQFKPDPAIN
                     NIVSSWPGRIDNDPALKLGFQADRLFEDFIRQYLQSYQSRP"
     gene            35346..36809
                     /locus_tag="PKM27_01620"
     CDS             35346..36809
                     /locus_tag="PKM27_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNR05980.1"
                     /translation="MSSFSFPIVGLVVAVFVLIFLVLRTRVHALIAMILAACIAGFTG
                     GLSVTATLDAITRGFGSTLGTIGIVIGLGVMMGRILEISGAADQIAHFFIQWLGRRKE
                     EWALALTGYLVSIPIFADSAFIILFPISSALAKKAGLSVLSLGVALAGGLIATHTLVP
                     PTPGPLGVAGLFGVDVGEMILLGLSLAVPCTLSFVWYAKWLAKKYPGFIDPAPTSEIP
                     EPDYEKHFNDRTGKALPGLTVSLLPIVVPILLIMIKSAISLFPSLSADQSAPVYWIVT
                     AFHFLGSPIIALCISTLLAVYTLVPHLDRRQTATKLEEGLQSAGIILMVTGAGGALGF
                     VVRESGTGAQIADQISRLPFSPVMIPFFVASAMRLIQGSATVAMITSASITAPILQQV
                     PGVNMLFAAQAAAIGGFLFSYFNDSLFWVVNRMIGVTDTRQQIMAWSVPTGISWAVGG
                     ILIALINLIWGSGGSWLDPVIPGLILAILLATAFSTK"
     gene            36831..37559
                     /locus_tag="PKM27_01625"
     CDS             36831..37559
                     /locus_tag="PKM27_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05981.1"
                     /translation="MEKSKTTLAEKYLSLDFSEKDSIGLTEQKTLRILIGVLGVLLPL
                     LLYLSLYVDSGHTVPLESISHYYYTRANSFFTITVSLLAVFLLIYKGKAAVDFYLSAL
                     AGIFALFLVLFPTDNLSLVCCDQEKPYAVTLLPGNPLREGFHYLSAAIFLTSLAVLSY
                     FIFTKSNKPPGERSPGKIYRNLVYRTCGLLMALALLVVLAGTLNLIHEPYYTGHHLTF
                     WMETLAVECFGISWLTKAEVFFKD"
     gene            37661..40726
                     /locus_tag="PKM27_01630"
     CDS             37661..40726
                     /locus_tag="PKM27_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05982.1"
                     /translation="MSIQNPIKRLPLLTGILLFICACQTKAPQDDRLYTGDLFHENIR
                     STDPRTPEEERQGFKLPPGFEIQLFASEPDIDKPVNMAFDAKGRMWVTQSFEYPFPSP
                     PGKKSTDKLTILEDTDHDGRADKFTLVSDSLNIPIGLLPLSDGHLSFSVPHLYRFTDA
                     DGDEKPEGSRILLGPFGYEDTHGMVSNFIRGYDGWVHACHGFTNLSTVAGRDGDSIRL
                     QSGNTFRFRLDGSRVEQMTFGQVNPFGLAYDERGYIYSTDSHTSPLYQLIRGADYPHF
                     SKPEIMAFGPDMKPLENEATALCGIAYYGDTQFPEEFQGNFFIGDALISRVHRYSWTN
                     KGSTPLGKSETDFIQSADPWFRPVNIKLGPDGALYVADFYNAIIGHYEVPLGHPKRDK
                     QRGRIWRITYKGNHHDLQDLTQASIPDLLATLDADNISTRMAAADQLTDRFGEAALAQ
                     VRTVLDETGTLPRKYIHALWVTYRLGGMDDALLNKSLVHDSPLVRLHSLRILREWKPG
                     DASFQSMVARSLNDPDAHVQRAAAELLSSYPEISSVESALQILRAAPAEDTHLRYTAR
                     LNLRNLLRVESLMKTVAAKEWNEQDAGHIAGTLVDVHSADAALFLSNHLARYAFPKEK
                     IQLAYQQIMRFLPASKLDEVIRSAGAKQAEADLAARIFRGVKDGLAQRGGDAKTGLMQ
                     SWGTEIAKGLIQRYPADSISRSLAPQQLFAIQLAGEYKIRPLQPELVSFVENNPGHDL
                     KTAALSSLMKMSGDRSTLDLASKWLMSDSTPVTLKKNIATVLGESPGSDVNKILLGVT
                     NAPADLEVVVATALAGSPEGKDLVLQQVRNGAFKARTLIDPRVEEKMLFRISPQQQKT
                     YSELTAHIEPISEERQGLIEDRLEAFKLFKASPSQVDTGARLFNANCGICHRRQTESG
                     IGPQLHGIGKRGANAIAEKILDPNRNITEAFRNYTIKLKDGKILTGLYRGEQGAVVIF
                     GDLTGKEFSIRRADIVEQKPSRYTIMPDHFGTTLTQHEFNMLLSYVLTW"
     gene            40921..42108
                     /locus_tag="PKM27_01635"
     CDS             40921..42108
                     /locus_tag="PKM27_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNR05983.1"
                     /translation="MKKNTGPLFLLLILIYSFSPAQTPGKEVRFKKFHLWDEFYTEGA
                     TVADVNKDDRLDIIAGARWFEAPDWKAHDIWKHKKFDYTKGYSDSFLNFALDVNEDGW
                     VDLICHDFPGREVYWFQNPGNHEVLWKRHLIDSVASNESPMAVDVDGNGKKDLVFGNE
                     KTGQMNWFKAVVKEGRVTWVRTAISEPKAKGVGLFSHGLGWGDVNGDGLNDVMIIGGW
                     WEAPNDIKVSPWIFHEATLGKPCAQMITFDFDQDGDADVVSSSAHDYGLWWYEQLVEP
                     GANGDPKRTFITHTIDSSISETHSLVLEDINRDGLPDLVTGKRFFAHQGKGPGGLEPA
                     VLVWYELLKDGRNRPVWIKHLIDDTSGVGIQFVVEDVNKDGLPDIVIGNKNGVFYFEQ
                     EKK"
     gene            42160..43572
                     /locus_tag="PKM27_01640"
     CDS             42160..43572
                     /locus_tag="PKM27_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR05984.1"
                     /translation="MVTFEELREIHALSELPDEHLQWLLDHSVYQEFEDGALIGKYGE
                     AAENMWFLLEGKVTFYMNVNGRLVYYFTFENNATTGGVSGLIPYSRMKTYPGNSYATG
                     KVRSIALHKKFFHELEDLNPDFIQKLIGYMTERAKAFATTQLQQEKVSALGKLAAGIA
                     HELNNPAAAINGIASELTKRLNRNYQLTESLLAGHLLPEQIRDIGAMVQKKENDRAVT
                     PKPSLIQRMEIEEDLAGWLSRMNIPNPMEASETFSEAGYITEELEGLCRQVGKEAAVH
                     ALQWLENLLSSQKIIKDLGNASARISNLVGAIKSHVHMDRTGDIQSTHLHQDIENTLT
                     LFGYKLREKNITVRKKFSDHMVPVPAYVSELNQVWTNIIDNAIYAMDKNGELVIETAV
                     EDHRVRVSIIDNGPGIPKEIRSRIFDPFFTTKKVGEGTGIGLDMVNRAVKHHNGEIRV
                     SSDPGRTEFAIYLPFEEKQQ"
     gene            43596..45257
                     /locus_tag="PKM27_01645"
     CDS             43596..45257
                     /locus_tag="PKM27_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR05985.1"
                     /translation="MKLPYILIVDDDLQVLRAIQQDIRNQYRNDYKVAATGSALEALE
                     LIKALKLRNETVALFISDQRMPEMEGIPFLEKAHALFPFAKKILLTAYSDIEAAIRAI
                     NLVKLDYYLQKPWHPPEEKLFPIVTDLLNEWHALYKPQQDALRIIGFQWSPKSHRLKE
                     FLSGNLVPYLWLDVERDADAERYLVSAQVSKSALPLAVLKDGAFLTDPSLAELAGRIG
                     LQSKASKEMYDVLIIGAGPAGLAASVYGSCEGLRTVLIEKSNPGGQASSSARIENYLG
                     FPTGLSGAELTQRAIAQTLRFGTEVLTPQEVKTITVKDGYKITEMMDGSLIHSKTIVI
                     ASGVAYKKLDIPGMDQFSGSGVYYGSASVEAHACRNEIIYIVGGGNSACQAAMYMSQF
                     CKEVNIVIRRDALSQTAAHYLVENIRNAPNIRVIPNTDVIGCAGSTVLENITFKNTLT
                     GEEKTLPAKALFVYIGMKPGTDWLNDLVLKNEKGFIITGNNLLQEKTYHTFWKLPREP
                     YMPETSIPGIFASGDVRFEAMTGISSAVGEGAMAIRFVRKYLQEI"
     gene            46389..47501
                     /locus_tag="PKM27_01650"
     CDS             46389..47501
                     /locus_tag="PKM27_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR05986.1"
                     /translation="MTNKKINIAIVGLGFGAEFIPIYQRHPHANMYAICQRTQSKLDE
                     IGDAFGIEKRYSDFAELLKDPGVDAVHINAPIHLHAEQSLAALRAGKHVACTVPMATT
                     VEECRAIVEAVQETGLTYMMMETVLYSREFLFVKEMYDKGEMGRIQFLRASHQQEMAA
                     WPGYWEGLPPMHYATHCVGPVLGLTRSEAEYVSCLGSGRIDEHLIPKYNSPFAIETCH
                     IKFRNSDLAAEVTRSLFNTARQYRESFDAYGSLKSFEWTQIEHEDHVIHTGEIPTRVK
                     VPDYAHLLPPEIASFTQAGVYDADSHQHLSFIQGAGHGGSHPHLAHQFLMALVENKDP
                     YPNAKQSANITCVGILAHESAMSGGQIIRLPEFTLS"
     gene            complement(47522..48460)
                     /locus_tag="PKM27_01655"
     CDS             complement(47522..48460)
                     /locus_tag="PKM27_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05987.1"
                     /translation="MNAPAGNDKILSWLLEGDVSIRYQVYRDLLGHEHKGLRNKIEKE
                     GWGFGFLNRRHSNGHWGRGFYQPKWTSSHYTLLDLKNLGISPANKAIKETLRLIFDKE
                     KASDGGINPIGSTQSSDVCVNGMLLNYACYFNVKEKHLKSVVDFLLGQQMKDGGFNCQ
                     SNRKGAVHSSLHSTLSVAEGIWEYEKNGYGYRLDELKKAAEESQEFILIHRLFRSDKT
                     GEIINPAFLRMCFPGRWHYDILKALDYFQQARVKYEPRMAEAIEVILGKRTDAGLWKL
                     ASPYPGETHFEMEKAGKPSRWNTLRALRVLQYYQFQ"
     gene            48609..50942
                     /locus_tag="PKM27_01660"
     CDS             48609..50942
                     /locus_tag="PKM27_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR05988.1"
                     /translation="MASFKIISLKSFLLLLVPYLLNAQTLDLPRSTPEAEGVSSEGIG
                     MFLDAWAKSKHEPHSFMMLRHGKVIAEGWWSPYRADLKHTMYSCSKSFAATAIGFAVT
                     EKRLSIDDPVISFFPESLPDTISPFLAEMKIRHLLSMTVGQQPDPTGAVVTQHDDWVK
                     AFLALPVLYQPGSRFLYNSLATYMLSAIVQKVTGEKVVDYLRPRLFEPLGITGADWES
                     DPHGINTGGWGLRIKTEDMAKFGQLFLQKGQWNGKQVLPASWVEEASTMKILQNPSAS
                     QALRDSSDWLQGYGYQMWRSRHNAYRGDGAFGQYIIIMPDQDAVIAITSETGDMQDEI
                     NLVWNHLLPAIKKDKLPAKPRASNLLKQRLAALSLPLASKTPAPAWSKTISGKHFLLS
                     PNDQFLGSLRLDFQAESSKLGLFSQKDSFIFSFGSGKWISGETKRLGPSLVAGAKGHF
                     AGLPPSKIAGSHAWKNDSTLELSLRYIESPHHETWTFVFNGDKIRVETLSSNNRNRIQ
                     YKGAITPKPTRLIVRGDDMGYSHSGNLALIQSSKGIQTSIEVIVPSPWFPEAVKMLEQ
                     NPGLDVGVHLAITSEWDNIKWRPVSDCPSLRDPDGYFYPMVFPNKNYPGRSIRENAWK
                     IEEVEKEFRAQIELALKKIPRISHISGHMGCSNLSDEVRAMTKRIAQEYNLDIDLQAL
                     GVKYMSYSGPKNSSKEKIQSFINGLKNLDPGQTYLFVDHPGLDDAELRAIHHIGYEQV
                     AEDRQGVTDLFTNSAVRDFIEKNGIRLISYADLAKDK"
     gene            50958..51698
                     /locus_tag="PKM27_01665"
     CDS             50958..51698
                     /locus_tag="PKM27_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR05989.1"
                     /translation="MHTDYDTIAKEYQASKLLPWRQHTEAYTFFKLIGNLEGLQVLDL
                     ACGEGFYTRQVMLRGASRAVGVDISAGMIQLAREAETSRPFGIQYHVCDVLELDLGQT
                     FDLVCASYLLNYSRTAAEIGQMCAVISKHLKPGGRFVTINSNPDHRCPPGALRHYGFT
                     RENESYQESAEVIYRFYQEDGSHIAVTNYHLEKKTHEAAFLKAGLTDVRWHSLELSPE
                     GLQEFGSDYWKAIVDCQPVIGISCMKPR"
     gene            complement(51785..52945)
                     /locus_tag="PKM27_01670"
     CDS             complement(51785..52945)
                     /locus_tag="PKM27_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNR05990.1"
                     /translation="MQAERPEGHAARIRSIDVIRGLVMVLMAIDHVRVYSGIPAGGPD
                     PGVFFTRWITHFCAPGFAFFAGTSAFLYGLKKGNAGSLSRFLLSRGLILVVLELTLLR
                     FFWAFHINYQEFILAGVIWMLGWCMVLLATLVWLPPRVVGLIGTAVIVFQSWFSRVPQ
                     VLPESIRASFGKGWEFIYPSGFETFSGVSVLYSLVPWIGVMAAGYGFGYILSLGEIQR
                     RKAALRTGLLFILLFIILGSIQIIRSPEDTDAAPFLFRLLNQQKYPASPLFLLMTLGP
                     LLALLPVAEKLKGGISKVLEVFGRVPFYYYLLHIPLIHLSALFIYKIKTGSMHQEFFT
                     YAPYAQVPQEYRWSLPLLFLVFAVNVGILYFLCKRYAGYKARNPGKKWLTYI"
     gene            53137..54114
                     /gene="gguC"
                     /locus_tag="PKM27_01675"
     CDS             53137..54114
                     /gene="gguC"
                     /locus_tag="PKM27_01675"
                     /inference="COORDINATES: protein motif:HMM:NF040903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GguC family protein"
                     /protein_id="HNR05991.1"
                     /translation="MRLIQLFHKQGQRRLALVQGDDLVLILNYTTAYDLACAAIDQQK
                     NPMDCVGQNLSDEKLPYEGIYDGRSEWKILPAFDHPINPRYCMLSGTGLTHKASAENR
                     QKMHELQEKGSLTDSMIMYQWGLEGGKPANGRSGVQPEWFYKGNGSQLRGHLDPLVIP
                     SYGLDGGEEPEIAGIYVNDEYGNPHRIGFATANEFSDHAMEKKNYLWLAPSKIRNCSL
                     GPELSLSQHFKSLSGKVSIARKGKTLWEKTIHTGEDHITHSLENLEYHHFKYDNHRLP
                     WDVHIHFFGADAFSYGAGIELEHGDLMSIQWEGMGWALVNPLHSTALVH"
     gene            complement(54154..56142)
                     /locus_tag="PKM27_01680"
     CDS             complement(54154..56142)
                     /locus_tag="PKM27_01680"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR05992.1"
                     /translation="MAPNAASPMPVKKEAPKQGSPAAPDPGNHKKQRPEQSGVSFENQ
                     HLYNPKVFTILSLAVCLFMCLMSFDYGVNGDEKFQVSYSKKLWSYYSTFGEDTSALYV
                     PEGNMHLYGGLFDLTAISLNKLLGLDNEYTPEFHYTRRVLCALFGFITILFSALFARQ
                     VAGWPLAFMTLVFMLFSTRFFGDCMVNPKDVPFAAGYIMALYFIVRFIRELPRPGISS
                     MSGIVFGVMIALNTRAGGLLLIVYLFFFTALLLTRKAIKKESFSNKELVISIGYTLVV
                     GFLAYLAGILFWPYALQHPFTHPLKALYEFTRQPITMNVLFNGEHINSARLPWNYLPE
                     WILRTIPLFSIFGLFFFIRLLKQRLQDIPFFEGSVLAFAAFFPVFYAIFKDSTLHDGW
                     RHFIFIYPPLVLIATFGWYLWFTSFRSKIFRIVSFLLLAGLLSENLYALVSLHPYQYA
                     YFNPLFGGIKKAYGHFETDYWMLSIKEASSWLKKQERVELNKKRHTVITNGMYPAQVY
                     LGDSSNNMDLKYTAYYYRSEKYWDYALFYSRFINRNQLLNRSWPPKGTIHIVKAGGVP
                     LCAVVKRENQEDYLGFEAMKRNDYKAAIHHLVEALEYTPYNETVSYALAVIYKNICEY
                     DKAERAIEMSLNAYPNNPAGIQLLNDIKLEKENYLFKR"
     gene            56143..56913
                     /locus_tag="PKM27_01685"
     CDS             56143..56913
                     /locus_tag="PKM27_01685"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR05993.1"
                     /translation="MYYLKLTKWDPPVRNGRIEFFPYISSPLPPDSSGGGPKMIARLK
                     KLLSNPFFYHYSRQVLLLGMPLKTWAAYGNYYNKNERIADLGCGPADILRYTDPPNKP
                     AFYLGIDQSGRYLKRAASRAAQKNISSAFINLNLDLLPSNEGIKTQLIRSLDEYQITT
                     VNLFGVLHHLNDLAVVTTLNAVFDSASVRSLNTQDVLFLARNPINNFYASLDRGEYVR
                     TEAQYDALLNKSKWQKMDKAWSRAGVRKVKYIHYRLKK"
     gene            complement(56935..57648)
                     /locus_tag="PKM27_01690"
     CDS             complement(56935..57648)
                     /locus_tag="PKM27_01690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR05994.1"
                     /translation="MFEITLPVLNEEKNLKTQVLILHHFLLTEYSLYPDWKIVIADNG
                     STDNTQGVGEKLAEEFARIKYIRVGERGVGLALQSSWSQSKAPIIGYMDLDLATNLKH
                     LPEAISALTSGFDLVYASRLAKGSVIKGRKFIREFSSRAFNMIARSYLGVHFSDGMCG
                     FKFMKREVYEKLVAGGARSKTWFFSTELLSVAEWQGFRICELPVEWTDSPESHVRILP
                     LAVQYLKAMKKLKRYKPRN"
     gene            57962..58213
                     /locus_tag="PKM27_01695"
     CDS             57962..58213
                     /locus_tag="PKM27_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05995.1"
                     /translation="MYQNYPNPFDQGTVIGLRLAQEGWGTLSIHDVTSKTIRSIESDW
                     TKGYHEVWPDRREIGATGVLYYRFESGFFTDSRKMVLIE"
     gene            complement(58326..59024)
                     /locus_tag="PKM27_01700"
     CDS             complement(58326..59024)
                     /locus_tag="PKM27_01700"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNR05996.1"
                     /translation="MKLKSFIPERWYLRYLKPLEMHIVQSRGKRIYSQEGEDILTDRL
                     LGGKEIGFYIDVGAHHPRRFSNTYYFYRKGWSGINFEPNPELFKFFLKERPRDLNFNT
                     GLYRKPAQLKYHYFEEPALNTFSVDAAAQYQAGNHKLIKTETIAVTTLSKALEGVQVP
                     EVIDFMSIDTEGLEGDILRAIDWNEIKPEVLLIELKYETVEQLLDSERVKYLKGFQYR
                     PVSKLMNTVIFKKE"
     gene            complement(59021..59866)
                     /locus_tag="PKM27_01705"
     CDS             complement(59021..59866)
                     /locus_tag="PKM27_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05997.1"
                     /translation="MKPSRLFIAADAARTEIPGEKELCEEVKHIVSGIDWPCEVHKLY
                     RNQHLGCKNAVSGAITWFFDQVDEGIILEDDCLPSVSFFSFCTQMLGQYKNENTIFHI
                     NGNNFQRAILRGDGSYYYSKYAHVWGWATWKRAWSHYSLELKNWETFKQSEDYIKQFT
                     SPEEKRYWSGKFEKYQRKPAVNNYWSYQWLYACWYYGGISITPQKNLVTNIGFGMGTN
                     TIIALHHLIIKAEEWPPPYIKPKDRSVDKEADDYTFKNIYKYKGPISSRIKYHLHRIF
                     GKRMP"
     gene            complement(60061..60879)
                     /locus_tag="PKM27_01710"
     CDS             complement(60061..60879)
                     /locus_tag="PKM27_01710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR05998.1"
                     /translation="MNDSNKPWLSIITVCYNDAKNLKRTLQNIREQKIKRVQLIVVDG
                     DSSDHTASVIQDFQDVITDWISEKDEGLYFAMNKGIRLAKGDYVVFLNAGDVFHDPQV
                     ISYIYNHHENKDILYGDAVFVDQEGRFKSRRHKKIPKELNWLSFRNGMVICHQALIVK
                     KTKTLFYDTQYQVCADLDWAIRITQGAESTRNLGITVCDFQRGGISDQRRNTALLERW
                     RIQQKHFGLKATLWSHLKIPMAFLLWKWEGLKWKVPAVAFQIKIEKASLNKNGT"
     gene            complement(60892..63345)
                     /locus_tag="PKM27_01715"
     CDS             complement(60892..63345)
                     /locus_tag="PKM27_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR05999.1"
                     /translation="MAASILAGINLVFFSPELSGKVLFQSDMINNRGMAREAMAHFEK
                     TGRPTYWTNAMFGGMPTYQIYVPQTKSNLFEFLIKGLSLSFSGSLKYYLILSLGAFLG
                     LCCLGMGPWLSLIGAYSIAFATNHVVLVSSGHLTKIGALGFVPMILAGAYLLFNKNWK
                     WGSVVFAFGLAGSLRMNHIQMTYQAGILLAIYVLAETILRVKKKRVNTLIRPALGLLF
                     GVIASFLVNYSLLMGMRSYAGETSRGGSVLSGHTAKESDGSAGGKNGLDWEYAMQWSE
                     GYKDLMSLLVPGAVGGSTNEVWSKKSKMAAALEKNGIAVPDRFTLPMYWGELPNTKGP
                     DYLGILLVLAFIMGLILVKGPLKWMVLTAAAFLVLQSLGHHFSILNKPLFEFVPYYNK
                     FRAPNSILNVLSTLVPLFSIYGLYLFMQHAWTKKSILSLFKKTVLPLAGALILILVLG
                     PQIFEMKSDLGDPDWKTNRELYEALLQERRSYLNSDTVRALVILFIGAGLLYLYALKK
                     LKPQDFILAFGLLVGFDLWMVAKRYVNAGNFVTEPSIHSIFKPRAVDQEILRDSSLSY
                     RVLDLSVNTFNTAIPSYFHKTIGGYSATKLSRYQDMVERHFSQNNRNALNMMNTKYII
                     DKKGVLEQNPDALGNAWFVSRVNTVNTPREEIEAINSMDPAEEAVLLQKEFKNLEVLP
                     SYQKNGIIRLVQYSPDHLIYETETSSEQFAVFSEVWYGPDKGWHARIDGRPANHVRVN
                     FLLRAMPVPAGKHQIEFKFAPPEMAANNISFYAGTFLGLWVLTSIGTALKKQWTPIPD
                     LDGPQKNTDRHPSGSGKTG"
     gene            63584..64066
                     /locus_tag="PKM27_01720"
     CDS             63584..64066
                     /locus_tag="PKM27_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNR06000.1"
                     /translation="MKLSKFGYLSKHRMALLYGMALAFLLFLLKWLQWRFLIVDHSIE
                     IYIGLIAFLFTLLGIWIARKLTKPRLKTVMVEKTVLIPADPGFKLNENELSRLNLSKR
                     ELEVLQLMAQGLSNEEIASRLFVSLSTVKTHGSRLFEKMEVRRRTQAVEKAKRLQIIP
                     "
     gene            64177..64716
                     /locus_tag="PKM27_01725"
     CDS             64177..64716
                     /locus_tag="PKM27_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNR06001.1"
                     /translation="MKKNILIFGLIMGAVLSANMVYTVNLCYHSGDFKGNDILGYAAM
                     IVIFSFIFIAVRNYRNKFNGGLIGFGSAFKMGALIAFIAATCYVLTWLFYYYLFVPDF
                     LDKYIPYVLQNAAEKGASASEVAKQTADMDKFKEMYKNPLFVILVTYAEVLPVGLIVA
                     LLSALILKKKTKDPEMAVP"
     gene            64782..65831
                     /locus_tag="PKM27_01730"
     CDS             64782..65831
                     /locus_tag="PKM27_01730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNR06002.1"
                     /translation="MKRIESMLDNLFRPLEFIGHRFILAPFRALLKKIDPLAEAIEGT
                     WLKKSLQVTIFPLFMILTVWVGFKLVEGGYTIRTFGGTIVMFLIMGFIFAPLERLLPF
                     SKKWQNDEDMPVDVLLFFGNKVWGDYINKPLQLATISLVVQKISPEIGRDLWPGFLHP
                     ALQVFVLLSVNDFFRYWYHRWMHENEFMWRWHAVHHSSERLYWFNGTRSHPLEGLVSN
                     FLWAIPLAFVKAPVEIVFVTGLLGRTIGRFQHTNMDVILGPFDYIFSSPKNHRYHHSK
                     KIEEGNSNYGGDVIFWDLLFGTFHMPRGQQPGDDIGIDQMPNYPKTFIGLMLAPFTYG
                     RIKKEAKKIMEKGGS"
     gene            66053..67063
                     /locus_tag="PKM27_01735"
     CDS             66053..67063
                     /locus_tag="PKM27_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNR06003.1"
                     /translation="MHSGSHYSFKEVLLWTRRDIYLHLVIALFPTVLYVLFDWKWLAL
                     PWLPIALLGTAVAFVVGFKNNASYDRLWEGRRIWGSIVNSSRSWAILVKDYVTNRHVL
                     NKLTVGGLSAVHKTLYERHFAWLTALRFQLRERRPWESIYKIHNLEYKNKWFRVEEHE
                     TRLADALRPYLSPEEYEVVMSKTNKAVQIIALQSSHLKELLEQGLIEDFRHMELEKIL
                     VELYAHQGASERLKNFPYPRQYATLNLWFIRLFIFLIPFGMFQEFEKLGDHLVWLSVP
                     FSALSGWIFTTMEKIGESSESPFEGSANDVPITAMSRNIEIDMREIFRGNPSTQTYSN
                     SE"
     gene            67119..67736
                     /locus_tag="PKM27_01740"
     CDS             67119..67736
                     /locus_tag="PKM27_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNR06004.1"
                     /translation="MKITILGTGNVGSALGKGWIVAGHSVLFGARNSASPKAQKIQDL
                     LPDVRIQSVEDAVKGAEVLVITTPPEVVLDLIPSLGDLSGKIIIDATNSVRTRPGPYP
                     TAFHAIKALAPSAEVVKCFNTTGFENLADPVYQGTGIDMFAAGNSLVAKQAASKLARD
                     LGFETCYDFGGDDKVELLEKLALSWINLALMQGLGRNIAFKLVKR"
     gene            67851..68741
                     /locus_tag="PKM27_01745"
     CDS             67851..68741
                     /locus_tag="PKM27_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNR06005.1"
                     /translation="MKSIYFLLFCILILMPRPSGLHAQAADGKLRIIMFGGHPDDCDQ
                     KGGGTAALWAKMGHAVKFVSVTNGDAGHQNMKAPELAKRRYEETQECARRLGVSYDVL
                     DNHDGLLVPSLDVRLQIIKKIREWNADVVLAPRPNDYHPDHRYTGVLVQDAAYMVAVP
                     LIMPEAPALKKNPVFLYFQDHFQRPNPFRPDISVDITSVFDQKIQCIDAHESQMYEWL
                     PWIGHYADQVPADKADRVKWLTKRLSRPIDAKIKATLVKWYGSSRAGQVRHAESFEIC
                     EYGSQPNAEEIKRLFPMLGK"
     gene            68752..69456
                     /locus_tag="PKM27_01750"
     CDS             68752..69456
                     /locus_tag="PKM27_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNR06006.1"
                     /translation="MKVRFFIPVILSLYALGVSGQASEIPVWMVKKTPDFTFSGNGAS
                     PEWTKTDWLELSRRKNDQVAYQTAVKLLYSETGMYCLFRCEDQTITATMKEDFLDLWN
                     EDVVEAFFWPDRSFPVYFEYELSPLNYELPILVPNHKGNFLGWRPWHYEGDRKTRHAT
                     HVHKNGGDIQSWTAEFFIPFVLLKPLPNSIPQKGSRWRANFYRVDYDQGSTTWSWAQV
                     RTNFHDYERFGILVFE"
     gene            69513..70559
                     /locus_tag="PKM27_01755"
     CDS             69513..70559
                     /locus_tag="PKM27_01755"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR06007.1"
                     /translation="MRSVFFLLFFIHLGFLFAQPAAYDTIILRHFKEQNLASLSVGLI
                     ENGKISYTKSLGMANLETLSPATDTTVYKIGSLSKQFIAAAVVKLAWEGKLSLTDPVN
                     KFFPDAPGHWKAITVTHLLNHTSGLRRESPAFDAMENKADLDLIRASYGDTLVFETGT
                     RWQYCNLGYFMLAEIVRQVSGQAFSTYMEQQIFQSAGLLKTRTTTVSELVPARADGYW
                     LGVTKQWLNSPNYIALRPSGAFLSTIPDLLKWELAIQQNQFLSKEAWSMLWRPGTPTG
                     NKQTDGTGIDYGLGWNVSRFKNHLWVYHGGSLPGFRAIYYRFPEENNAILILSNTDHA
                     NLGRLANDLAELWY"
     gene            complement(70590..71543)
                     /locus_tag="PKM27_01760"
     CDS             complement(70590..71543)
                     /locus_tag="PKM27_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR06008.1"
                     /translation="MISKEVTLPGSLRANEKIKIYSRIDGYIKRIYVDLGTPVTKGQV
                     LAEIDAPELLMQWAERNERKHAAYAQYLTSKDESERAADLARTPGTIPISEVIQARNK
                     MIADSLNWEAETFSLSILKESINFLQIRSPFSGVVTARYSDEGELTGSGGKQHILELE
                     ANGLMRVEIGVPETYSSAVLKDKNLSFTVPSIPGRVFRAVFARRSNALTENTKSEIWQ
                     FDLANPEGQLKSGMYVQAKMKLGRKDSTFLIPPDALVTTQESQYLVGIRSDTTWFIPV
                     SLGFSWKDQIEVFGGLSRGDRIVRFANDLIREGEKIKQSNE"
     gene            complement(71677..74793)
                     /locus_tag="PKM27_01765"
     CDS             complement(71677..74793)
                     /locus_tag="PKM27_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR06009.1"
                     /translation="MNLIVAALKKPVTVLVLVLAMILSSVLSLKKVPLDMFPDLGLPT
                     IYISQPYGGLSPEQMEGFVTSNLEYYCIYLTGLAYVESKSVQNYALIKLQFHPGTNMN
                     QAMAEVVNNVNRAKSKMPEGTVPPFIVRYDAGSVPVGQLVFSSENHSLTEIQDLALFK
                     VRPMFSSLEGVSAPPPFGGNQKAIIVKVNPEKMTSLGISPDEVVDAIAAGNMVTPAGN
                     IRIGDENLITPINSVAGNIKDLENLVVKKGDRFMAFIRDVATVQNGSDLTTGYALING
                     RRSVYIPVSKRSDASSWQVVQNVKKALPAMQAAVPDDIRVTYEFDQTEYLIKSLKSLL
                     TEALLGALLTGLMVLFFLKDLRSAFIVVLTIPLALLGAVTGLSITGQTLNLMTLGGLA
                     LAVGILVDEATVTVENIHRHQESGKPKRRAILEASLEISFPKLLILLCILMVFVPSFF
                     MSGLPKAMFQPLAMAVGFAMIISFLLSQTFVPVISAWLLNNRLSQHHNGKAFRMQQKY
                     RERIAGWMAWKRPITITYFLILALSLVGLFASIGTEIFPRADGGQFQFRLRLKPGTRM
                     ERTEEATRKALMRIETLAGRENVEITSAFVGAQPSSFPNNTIYLWTNGPYEAVVQVKL
                     NSASGVSLADLREKIRDDFSKNMQDVSISFEPADLVERVMSLGSTTPIDIAVLGRNME
                     QSKAIAESLMVKLTTLTYLRDLQIGIPLDYPSLKIDIDRERVGLMGLTIEDINNAIVT
                     ATSSSRFVSPVYWLDQESGNTYQIQAEFPQNQMNSIESLENIPLGKEGMRILLRDVAR
                     IFPASSYGVYQRYNQQRMVNVTANLEEKDFGSAMKEIDDILNTLELQPGTTILKKGQS
                     LVLDQTLSELKNGLLVALVAIFLLMAANFQSLRIGFVILLSASASIVGALLLLTLANQ
                     TLNIQSFTGTVMALGVSFANGILMINAAEGFRKMGLSPENSISEAAGTRLRPILMTAV
                     AMIAGMIPLSVGMGETGKQIVPLGLAVIGGLTLSTIITLWILPSIYGSFMTGVGNHSI
                     SLLPGDEQ"
BASE COUNT        18921 a        19231 c        17569 g        19362 t
ORIGIN      
        1 ttttttggaa tccataccgc aaatctttgt cggattattg atcgaatcca gtcaccaaaa
       61 aataaaatca aagcagctta gatcatataa ataaggatca ctcaggcgaa tgggatggaa
      121 tacctgtgtt ttccaccaag ttatgaacat actaacagcg attttcctct tgaagcgcta
      181 ttaaattggc ttgtttgacc cggaacaacc catgtttata gaatccacta ccacaataac
      241 aagaaaataa aattcggtcg ttctaaaaaa tgaccttact cctgaatttc cctgctttaa
      301 tgcgatatcc ggagagcaag aaaaggccga tcaggcagta tcgaattcct ttatctcttc
      361 ggatatcctg cgatcccttt ggcttcttcg atctgctggg tatggcggtt ggaatgtccg
      421 gcaatgaaga ttacgaactg gtaggcgtcc atttttccaa aagcgcgggt cactatatga
      481 ttccgaaagt ccagatccga atttttgatc agctcagcca gggcctctct tctctcttca
      541 aaaaccgtcc aggcttcttc aaaggattta aagccggtat tgaggggctc aaaaggcgct
      601 gcggttttga acttgctgct ccggtccatg atattgtttt taatctggag atcggtggtg
      661 gtcaggcggg ctttggtggc gggatcggcc ggggcctgga gggctgcatc cagggtggct
      721 ctcagggctt tttcagaata ggcaatatga tataaacatt ccagcgggga ccaggcatcc
      781 tcggcaggat ggaaattcaa ctgcgctgca gaaagtcctt tcaggctctt ttttaattcc
      841 ttggttgtcc agtcgataaa ctcaagcgct tcctttcgtt catccctggt gagactttga
      901 gtgaacgcaa gctgaccaaa caagatggaa aacaggaaag cgatggtttt tttcatgcaa
      961 agtattttgg tagttcaaag gtaactgccc tcgttcatac tggttaatcc tattcgaccc
     1021 gcttcggggt cgcgggatac ccggttaacc cggaggctgc gccttcggtt agtcgtttta
     1081 gaggttatta cttttacctg tgattcgtat tagagtttag ggccattcac caaccactga
     1141 ctaccaatca cccctcagga ctttttattc atcaaatact catcaaacca ttccgcgttg
     1201 ctctgcaggg tcttcagaac cattttgggt tcctgcggtc cgtgcggttg acggggtagg
     1261 gccagcaggc ggaccggcac ttcccttctc cgaagtgcgt tgtaaaacat gaccgcattg
     1321 ctgaagggca cccgtttatc cgcctccccg tgctgaagca gtacaggcgt attgacattc
     1381 tgcacaaaac gaaggggaga atgtttgtca tacaaactcc agtcgttcca gggatccgct
     1441 ttgaaatagg agggtaaaaa tccttcaata tcatcggtca ggttttgatg tgccaggtcc
     1501 acgaccggag ctccgatgct ggcggcctta aaccggttgg tatgtcctac gatccagctg
     1561 ctcataaacc cgccataact ccatcccatc acgcccatgc gggaggtatc ggccacacca
     1621 atgcggatga cttcatcgac cccgtccatg agatcttcat aatccatacc cccccagtct
     1681 ttccggttgg ccatgcggaa gtccgctcca tatccggatg aacccctggg attgggtctg
     1741 aggatcgcaa atcctttttc agcgaggccg gccaggggat agactgcctg gttggaggca
     1801 atacaggttt gttggaatac cccggcgggc ccaccatgta catttaatat aaaaggaact
     1861 ttttgcccgg cctggtaatt caccggataa gtcagcagac cttcgatctc tttaccgtct
     1921 tttgatttcc agcgaatgac ctcggtcctg ggcagggttt tggatttcca ggcttcgttg
     1981 atattggaaa ccagtatggg tttgtattca tggatggtgc ttagataaac ctgaggcagg
     2041 gtacttgcat tctgaaggac aaaggcaaga tgaccccctg ttttgttaag gttcgcagca
     2101 ctgaggtgca tgcgactgcc cgcattccat tcctttgcag aactcccatc gacggaaaga
     2161 atatagacgg agctcatcgt tttattggat tcattgacca gtaaagaacg gctgtctgcg
     2221 gtccaaccga tcaacgaagc gttttcatca ggtgtggctt tgagcttcca ggtctttccg
     2281 tcagccaaag aataaatccg gatatgttcc ggaccggacc aatccacagg atccgtggtg
     2341 ctggcgtagg ctaggtactt gccatccggg ctgaacaggg gattggactc gcccgcaccg
     2401 ctatttgcta tggattttat tttgccgtct tccaggttta ttaaggaaat atcgctatac
     2461 acattgtcgt tgacttcggg gcttttaccg tggctgaaga tgatttgttt tccatccggg
     2521 ctccagtcaa atgcattgac atttaaatcc tgttcggtta gttttttcat taccctggcc
     2581 ccggccgaat ccttttgatc cagcttcagg acatagagcc ggttttgttt tatcttttca
     2641 tccatataat accagtcctc ttttcccttg gtatttttct catcctcccc ggtgacgggg
     2701 tctgcaatca cacaggccag ggaccgtcca tcgggactcc atttataatt gcctattcct
     2761 gattttacgt ccgtgatttt ttcagcttcc ccgccggcca ccggaaggag gtaaagattg
     2821 tttttcccgt cccggttgga tacaaaactg atccattgtc cgtccgggct ccaggccggt
     2881 tgggccgagt ttttatcccc ccgggtcaat ggcatctgtt ttcccgtttt gatgtccgtc
     2941 atccagatct ggttgatgta ttcactgcgg tcggcggtca tcaccgcttc ccggactgca
     3001 tacaatactt tggagccatc gggagaagcc gtgactgcag taatgttttt gagttcaagg
     3061 catttttctg cggtccaggc ggattgggca agcagataat aaccgcaaga gagcagaaat
     3121 acggaaagga ctgttttttt catttgggtc aatggatttt ttgatttgaa gttaaggaaa
     3181 tttgagagct gaataccggg gtgaaggcta atactcttat ggtcctttga cccttacccg
     3241 ttgacctttt ctcctttgat tagccttcct ccccttccat tctcctgggc ttcatcggct
     3301 tcgcctgttt gatccgataa ctcagggata agttgacctg ccttaccctg ggccggatat
     3361 tcccttccgt gaagaaattg gggccctcgc tcactatacg catgcggcgg ctgttgaata
     3421 cgtccaggac actgaggttg agcgtgcctt tgtctttcca gaggtccttg ctgaccgaca
     3481 ggtccgcaaa atagagcgcc tttcgtttcc cctgggcggt tcgctgaggc gcttcataat
     3541 tggcccgtaa ctggatgtcc aatcctcggg gcagggtaaa gcgggaagtc tggcgggtaa
     3601 accagctgta ggtataggcc cggtaatccg gcaggatatt ggtgccgtcg atgtccgcgt
     3661 aaaaataatt gaaattgaaa tccagcttcc accagggtaa gggagaatac ccgctggtga
     3721 attccacccc caaggctcgt tcggataaaa ggttttcggt ccgggtgacc gaatttccgg
     3781 attcgtccac caggcggatg cgctcgatcc ggtcccgggt attgcggaaa taaacggacg
     3841 aagtcaggga tcctttttca aaagttttga tatgccccag ttccagggca ttgctgaatt
     3901 cgggatccag gtccggattg ccgctgaaaa aattgcgggc atccgaaaaa gtcacataag
     3961 ggctcaaatc attgtaaaag ggccgtctca cccggcggct gtagctccat tgcaaggcgt
     4021 tttcacgggg caggctcagt gtgaggtgta cgcttgggaa cagattgaca tagtcccggg
     4081 gattcacctg gttggtttcc tgcagggtag ttttgacatc ggtccattcc gcgcgcaggc
     4141 cggcctggta cgaaaaccgg ccgcttttat tccccaggat cccataggcg gcatggatgt
     4201 tttcatcgta aagaaagata ttatccagac ccggcaacgg aataaaggtt cctgtttcat
     4261 ttttctggct gaccacataa tcgttgacca tgtcccggaa actgcttcgg atgcctgttt
     4321 ccattttccc ttcctttccg atgggcctga cataatccag ttgcaacagg tattgtttct
     4381 caaactcgtc attgaccgac ttttggacca ggttccggct gaggtcctcc gttccatccg
     4441 ataaaaaaaa ttgttgcccg taggtttgat cggaattttc ccaataatcg atgtatttca
     4501 cttcggcaat caattcatga cctttttggt caaaagctct tttataattg attgaaaatt
     4561 cggaattggg ttcgatctcg tcctcatcct gctgtctttt actgagattc gggcttccgc
     4621 ccaggttttc gaggtaatcc tggtattgaa tgttgagtaa tcgattgccc tggctgcgac
     4681 ggtagaggta ggccgcagtg agaatgctct tttctgagaa aaaatagtcg agtccgccgc
     4741 gtacattgtt gttaaatccg gtgaaggtcc cccggttggt ttgttcgagc aggcgggtgg
     4801 tatcgccata tacttcctgg taaagggaac ttcggatcaa ttgatcccgg tacgaaatac
     4861 cgtaattgat aaaaaaattg agtttgttgc gccggtaatt aaaattggct gccagcccga
     4921 aattagccgg gttcccggtg atcaaatcca cggagccgtt gaatccctgg ctgcggtcct
     4981 ttttcaatac aatattgata atgcctgcca tgccctcggc ttcataccgg gcggaagggt
     5041 tggtgatgat ttccactctt tcgaccaggt tggcctgcaa ctgctggagg ccgctgcccc
     5101 ctttgatgct taccaggccg gatggtttgc cgtcgatcag gatccggaca ttatcgctgc
     5161 ccctcaactt gaccccgcct tccgcgtcca ccgacacgga gggaatgttg gtaaggatat
     5221 ccgaagcgga gcctccggca ttagcaagat ctttgcccac attaaaaatc tttttgtcca
     5281 gggcaagctg catggagctt ttttcggcct gaacgactac ttcttccagg gtgctgacca
     5341 gaggcgccag ctggatggtc cccaggtcgt gccgggaatg ttcttttgat aatataaagg
     5401 aagaagtggt tttccctcta aagcccataa aatccaatcg cagaaaaaac tcaccgtatc
     5461 ctgctgaaat cgcgaagctt ccgtcgtcgg tggagaggtc gccgttgacc aggctgctgt
     5521 ccatcaccga tagcacctgg atgctcgcaa agcccagcgg ctgtccgctt aaggagtcaa
     5581 cgatccggcc ttttacctgt gcatttttag ccgtctggcc ctttatgcca aaaggaataa
     5641 acaagaataa gagggaaaaa aaagaggcgg cttgttttaa tttcattgga tgcctttgat
     5701 ataagcccgc aaaggtaccc aatgtatttc cttaagggca tggccgttcg acggtccggg
     5761 aagttttttc gtcaatgatg ccggggtagg tgcagatttt gatcatatac tctgtttata
     5821 ttctaattcc gggctaaagc cccttacttc attaaggtca gcagacccca ggctaaagcc
     5881 atggggttgg ctatgttatc tgtctataat cccggttttc aggccagggg ttatgcgcca
     5941 accacatgat tgggctttag cctcaaacac ttcagattgc ttgttattag tttaatgagc
     6001 ttaccttagg ctaaatccaa aggctgcaat actgcctata caggtgctgc taaccctaac
     6061 ttccatatcc ccggccttta ggccgggggt tatacgccaa ccacatgatt gggctttagc
     6121 cccaggccgc tcgaatgctt ataatgtatc actatccgtc aattcccttt tataaataaa
     6181 aaccaaagcg aatccttagc tcggataagt cgcttatttt tatccccttc aatccacttt
     6241 catatgaaaa aactgatatt cttttttata gtcctggttt tagccgctga taccaatgcc
     6301 caaaaacccc tctacaccgc cccctttggc atccaggcct tcacctttcg acgcagtttt
     6361 cccaacagca tagaaaaaac cctggatgcc attgccatga tgggatttac agagatcgaa
     6421 ggcagcggcg gccgcattgc ccctgaagat ttcaggaaac tttgcgaagc ccggggaatc
     6481 agtattccgt ctaccggaac gggctatgaa caactggtca aaaaccccat ggaagtggct
     6541 gcccgcgcca aagcccttgg ggccaaatac gtgatgtgcg cctggatccc ccataaaaac
     6601 ggagctttta attttgaaaa tgccaaaaaa gccgtggagg attttaacgc ggccggaaaa
     6661 gtgttgaagg agcaaggcat tactttctgt tatcatgtcc acggctatga attctggcct
     6721 tatgaaaaag gcacgctgat ggattatatc atcaaaaaca ccaatccgga atacgtttcc
     6781 tttgaaatgg atgtgctctg gacccagttt ggcgggggtg atccggtcaa actactgaaa
     6841 aaatacaaag accgctggaa gctcatccat ctcaaggacc tcaaaaaagg agttaaaaaa
     6901 gacctgaccg gaggcacgtc caccgaaaac gatgtcgccc tgggcacagg agaaatcgat
     6961 atccccggca tcatccggca agccaataaa atcgggatca aacatttttt tatcgaggat
     7021 gaaagcaata atattctgag ccaggtgccc cagagcgtgg cttacctgaa gagcttaaaa
     7081 aaataagctt tttttcattg ccttgggatc agtaatcctg cagggatctc cgggagacct
     7141 tgactgctga gataaagaac cgttatcttt attaaatgac cccttttaaa ctcttttatc
     7201 attcagcctg tattttatgg atggtctgca tttcctcatc ctgtgcagca aggggagatt
     7261 cgccggactt gattctggtg ggcagcaccc ccggcgatga tttgatcaaa tctttactgg
     7321 ccattcctcc ggagactaaa attgacttta tcaaatggaa ccttgccctt agtccttcta
     7381 atacgggtca ggaaaccttt gtacttggaa tggtagtggg agaatcgcaa cccaataccc
     7441 tgggttttat cgggggcgga aagccgctta cgattaaggg gacctaccgg gtttctaaag
     7501 aaatgggcgg tcaccgggga gcggaaacag tcaggctgac cagtgaatcc cttccctccg
     7561 gaatttcgct ggcaaaactc aacgataacc tgtttcatct cctgaccccg gaaggcaaac
     7621 tgatgatcgg gaatgggggc tggagttatt cgctcaaccg gaaataccct gttcccctga
     7681 ccggaccatc cgcattttca aatcctttca agcccgccgg tgaaccttcc tttgaggttg
     7741 tttttgacgg ccgcaccccc tgccgggaga ttgctgccga acatccggaa atgaatgcga
     7801 gcccctcttg tttcaaatta aaatggagat taatcctgca ccgggactcc gcaaatcatc
     7861 ttcctacgac ctatacgatc cgtaaagtgg tggacaatga accccgggat gtgacgggta
     7921 catggaccat cctgaaagga attccctcca atccggatgc ggtcatttac ctgctcgatc
     7981 ccgacaagcc cgctgagtcc atttcattcc ttgccgggga tgaaaatgta ttgttttttc
     8041 tcaacaggaa tcacgagccc tatattggaa acgaagattt cagttttacc atgaaccgga
     8101 gaatccagta aaccgttcct gcccgatttt ccccaatcct tcggtacctc tggatactga
     8161 accttcctgt taaaatccct tcaggtaaac ttcttaattt cattcagcca aagcccgacc
     8221 ggtcatgaaa accatcccct ttttatccgt tttaatactt gcccatatcc tttgcggatc
     8281 cggaccggct aaggcccaga tcgagctgag ggtcaaaccc aacgagatcc gttccacagt
     8341 ctccccccag atgtggggca tcttttttga agacatcaat ttcgcggcgg acggcggact
     8401 ctatgccgaa ttgatcaaga acggatcctt ccagttccct ttgcccttca cagggtggaa
     8461 agtaaataag gtggatttta aagacaaagt gcttgtggtg aaccagtcca gcgtaaaaaa
     8521 cggaaatacc cgttatgtac ggattgccaa atcatcggac agttccgttt ataatatcgt
     8581 caatgaagga ttccggggaa tcggtttgca taaaaatgca ggatacactt tttctatgcg
     8641 ggccaggacc atcagcggga atctccgcct gcgggtcgag gtccttcgtg aatcgggaca
     8701 atccctggtc cggggcgata tcgccaacct caagggaggg tggactgatt acacccttcc
     8761 cctcactgcc ccggactctg aatcaaaggg tctcctgaaa attgcctttc tgggcactgg
     8821 ggtggtggaa atagacttga tctccctctt cccggtcaat acttataaaa accggcccgg
     8881 cggcttaagg gccgacctgg cggctatgct cgacagcctc aagccggggt ttgtccgctt
     8941 tcccggtggt tgcatcgtgg aaggccatac cctggccagc cggtatcaat ggaaaaaaag
     9001 gtgggaccaa aacaggagcg ggaattgtcc atcaaccggt ggaatatgga attcccaaga
     9061 agggcggcac cggactatta tcagtccttc ggcctgggtt tttatgaata tttccagttg
     9121 tcggaagatc tgggcgccga acctctcccc atcctcaatt gcggcatggc ctgccagttc
     9181 aacagcggcg aggtcgttgc catggatgac ctggggcctt atatccagga tgccctggac
     9241 cttattgaat ttgccaatgg ccctgtacaa accaaatggg gcaaactccg tgcggacctg
     9301 ggacatccgg ctcccttcaa cctcaagatg atcggcatcg gaaacgaaca atgggacgaa
     9361 caatattttg agcgttatca aaaatttgaa caggccctga aagccaaata cccggctata
     9421 caactggtct ccggttcggg cccttccgcc cagggaaatc tttttgatta tgcatggtcc
     9481 caattcaaag attccaaagc cgcctttgtg gacgaacatt attatttgcc cccggcctgg
     9541 ttttttgcca atgcccgccg ttacgaccat tacgaccgca accgccccaa agtctttgcc
     9601 ggagaatacg cggcccacga caaggaggat gcccagccgg aatcgcgcaa cacctggcta
     9661 agcgccctgg ccgaagccgc attcatgacc ggattggagc gaaatgccga tctggtgcgc
     9721 atgtgctctt atgcccccct gctggcccat gtggaggcct ggcaatggag gcccgacctg
     9781 atttggtttg acaatcttcg ggtcatggcc acaccgaatt attatgtaca aaaattattt
     9841 tccaccaacc ggggaagtca catgatcccc attacccggc aggattcagt catagcgggg
     9901 caggacagcc tttatgcgtc cgctgtcctg gataaaaatc aaaaccaggt aattatcaaa
     9961 ctcgtgaatg cggcgcgaaa ggcacaaaaa ataaacatcc tcatcgacgg gttgcccccg
    10021 gataaattcc aggcccgggg cacggtcttg tcttccaatg cggacttggc gtttaaccgg
    10081 atgaatagcc cttcggtcat aacgccaagg gaaaccagct atacgattga gagtggggtg
    10141 ctgacggagg atttcccggc gaggtcggtc caggtgattg ttttgcagta tgggtctatc
    10201 cggcgatgag ggttcgcgtg gagagtttca cacggagaac acggggaaca cggaggtatg
    10261 tttttataaa aaaaacggaa agacttttta aaaacgcctt gagctctacc tgcccagtgc
    10321 aaaaaattcc atagcttttt ataaattccg tgctctccat ttagtttcgc acggagaaca
    10381 cggggaacac ggagaaatat tttttataaa aaaacagtaa cgctaattta aaactccgtg
    10441 agcaatgtct gacccttgca aaaactccag agctttttat aaattccgtg ctctccattt
    10501 aaaaaaaaat gaaagtactt aattgcacca ctcctggcca ctttgaatac caggacaaac
    10561 cagaaccagc atcccgggca ggttattctc tactccgcat acaaaaagtc gggatatgcg
    10621 gtaccgacct tcatgcctat aaaggaaggc aaccttattt tgaatatcca agaatccttg
    10681 ggcacgaggt ggccgcagaa tatgtcagtg gggatgcgcc ggatatccgt ccgggggatc
    10741 tatgcacttt tctgccttat atatcctgcg gttcctgtat cgcctgccgg tctggcaaaa
    10801 cgaattgttg cagccggctt gtggtctgtg gagttcattt ggatggcgcc atggctgaat
    10861 actatctggt cccctctccg ctcatcatcc ccggagaaaa tttaagcgaa gacgaactcg
    10921 ccctggttga acccatgtcc atcgccgcac atgccattcg gagggcagaa aacatcaaag
    10981 gaaaacaggt gctgatcttt ggtgcgggcc cgatcggtat gtgtacatcc atcttggcct
    11041 tgcaggccgg agcggtggtt ggactggctg atgttcacgc gggcaggctg gcctttgcat
    11101 cctcctttta ttcaatccat accttcctca accgggatcc atcgcatttt ataaatgaag
    11161 tccgaaactg gaccaacgga gacatggccc cggtggtcat cgacgcgacg ggaaatttgc
    11221 aagccattga atccgggctt cagtacctgg cccatggagg acaatttata ctggtcggcc
    11281 ttcaaaaaga agcctttcat ttttcccatc ctgaatttca taaacgggaa gccaccttaa
    11341 aaagcagccg gaatgctacc cgtaaagatt ttatccgggt ccgggatctg ataaaaaata
    11401 aaaccctatc cgtagatccg ctgattaccc accgcatccc ttttggcgtc gtagcgaagc
    11461 agtttgaaac actttacgat cccggcaatc tggtcatgaa agcggtgata gagttttagc
    11521 ctgaggccgg gaatgtttgg atatgctgga aaaatagaaa cttccgggtt atacaaagat
    11581 tatcctctta tctttatttt aaggtttcct tatgaactct gtgtgaatgg ccagcgtctt
    11641 atttttatca aatacctttg ctgaatgatt gtgctttatt ttattttact ggctcctttc
    11701 ctttccggct ttttccagga aaaccagccg gttgaaaaaa tacagatcga cggaccctat
    11761 gtcgtgtaca aaaacgacct gatttatgtc cagtatatct accaggaagg gggcatcaaa
    11821 tcggtcacca cggacagttt tcccgtttcc cagaaaaaga acattgaact tcgggtagcg
    11881 acggacgagc ccggaaaata tttcaccgtt aaacttaaaa acaggctcag caatgaaaaa
    11941 tcagagtttg gcaaaccggc caaattgctg gccatctccg atatcgaggc taatttcaaa
    12001 gcgctgcgga cgctgctcca ggtcaacggg gtcatcgacc aggaattcaa ctggacctac
    12061 ggcaaggggg aggtagtgtt taccggagat tttttcgacc ggggcagcca gcagaatgaa
    12121 gtgctctggc tcatgtacgc cctggaagaa aaggcccggg aagcaaaagg ttatgtgcac
    12181 ttcgtgctgg gcaaccacga gatcatgaat atgaacgggg atttccgata tgtgcacccc
    12241 cgatatatgg aacatgccaa gatgatggga gtgccctata tctcctttta ttgggacaat
    12301 acggaactcg gccgctggct gcgcaccaaa aacatcgctc aacggatcgg cgatatcctt
    12361 tttgtgcatg gcggaatctc caccccggtg aattatatga atgtgccggc gccggcgatc
    12421 aacgaactgg cccgtccctt ttacccggat actacataca attatccgga tatctggacg
    12481 gagatcctgt tcagcgacct ggggcccttc tggtacaggg gttattattc gaaaaagtcc
    12541 cccgcaacaa gcggccaggt cgacaccacc ctcaatgtat ttggcgtaaa atacatcacc
    12601 accgggcata ccgtagtggc cgataccatc agcgtattgt acaacggcag ggtattcaat
    12661 accgatgtat atcatgccgg gggcaattcc gaggccatgc tggtggaggg aggtaagttt
    12721 taccgggtca acagcaaagg agaaaaattt ctgatgaaat aaatcgctgc ccacttcaaa
    12781 accgtttatc ggttattgaa ccttcctgaa aattttgatt tacggtatct tgacctcccg
    12841 taatgctgtc aatactatcc gcaaataaag aggtcagggg cctgtcgatc ctgctccttt
    12901 tttgttttcc ttttcttgca aaatcgcaga tccagtggcc cgaaatcggt agggacaccc
    12961 ggccgtggac gcgctggtgg tggcagggca gcgctgtgga tacgcccggg ctgaagtggg
    13021 ctatgaaaca ataccaggaa gccggtttgg gaggcctgga aatcacgccg atttacggag
    13081 tacagggcca tgaagcccgg tttatcgact atctctcgcc tcaatggatg ggggtgtttg
    13141 actttgtttt gagagaagca aaaaaactcg gcctggggat cgatatggct acgggcaccg
    13201 gctggccttt cggcggaccc tggatcggtg aagaccaggc ctgcaaatac cttgcgcata
    13261 aaaaatacca attgcgtgaa ggccaatccc tgaaagatac catccaatac ttccaggagg
    13321 gatttgtgcg cacagccaat ggggaacgcc tttcggtggc ggatgtcatt tatcccatta
    13381 ccgacaatga ggatctccag gcccttgcca ttgaccaggt caggcatccg gtggccttgc
    13441 ctctgatagg gttgttcgct ttcaatgatc tgggtcaatg gatggacctt agccaaaaag
    13501 taaacaaggg ccttctggaa tggacggccc cgcagggcac ctggaccctt tacgccctat
    13561 ttcagggtct tcatggcaaa atggtggaga gggccgcgcc cggaggagaa ggaaatgtga
    13621 tcgatcattt tgacgaagcg gccgtccggg aatatttatc cttttttgac cgggcttttg
    13681 aaaacacaga cttgtccggc ttgaggggat tttttaatga cagttacgaa gtagacgatg
    13741 cccgtggcca gagcgactgg acaccccggt tttttgagga atttaattcc aggagggggt
    13801 atgatcttaa agcccacctt ccttctttat tttcagaaac gccggatgaa aaaagcgcac
    13861 tcgttttata tgactaccgg gaaaccgttt cggaactggt ccttgaaaaa ttcacccgga
    13921 cctggaccca ttggggacat cagaaaaata aatggatccg gaaccaaagt catggatcac
    13981 cggccaatat cctggacctt tacggggccg tcgacattcc cgaaaccgaa ggcaccgatc
    14041 tcctccgatt taagtttgcc tcctctgccg cccatgtgct tggaaaaccc ttggcttccg
    14101 ctgaagcagc cacctggctc catgagcatt ttatctcctc cctgtctgat gtccgggcag
    14161 cagccgatca atattttctc ggcggagtca atcatctggt ttaccatggc accaattatt
    14221 ccccgcctaa tgaaagttgg ccgggctggt tgttttacgc tgcggtgcac ctcaaccccg
    14281 caaacccggc ctggaaagac tttaaagcgc tcaatgatta catcagccgc tgccaggctt
    14341 tcctgcaaaa aggaaatccg gaccaggata tcctgttgta cttccctttc gcggaccgca
    14401 accagcagtt gggtaattct gtgttgcacc attttgacgg catgcagggt tttgaaggca
    14461 gcatctttaa atccgctgcg gaagaattga tccaatccgg gtatgcctgg gacctgatca
    14521 gtgaccggca gattcaatcc ctccaatgtt tgaatggaaa attaatcgct ccgggaggcg
    14581 attaccagac tatggtcatc agcggtgcgg aatttatgcc catccagacc ctctttaaaa
    14641 tcgacagcct gatccggaaa ggagctaaaa tcatttttca tgtatatccc acccatgtcc
    14701 ccgggtttta tttgcacgaa gaaaaccaaa ggctgttgga ttccctggtg aatgcgctgc
    14761 aattaaaaaa aaaccaggcg gggaagttgt atatggatca cggcctcggg cgggtatata
    14821 aaggaccgga actccaggcc ctgcttgatg ccgcagagat aagatcggaa aaggccattt
    14881 atcaaaaaaa ccttgcctgc atccgtcgca agacggagac cggctattat tatttcataa
    14941 aaaatacggg tgattctgaa ttaaaagact ggctgaccgt aaatgcctcc gctgcttccg
    15001 tgcttcagtt tgatcctatg aaaaaccaat ccggcctcgc gcgctggaaa aaaaccgctt
    15061 ccgggaccga agtatggatt tcattgttgc ccggggaaac cgtgatcctt gaaacgaccc
    15121 gtcaaaaaat cgagggtcct tcttttccat tttttgaacc ggcaggagaa gccgttgacc
    15181 aggattcagg ttggaattta aaattcattt caggcgggcc tgcgctttcc gaaagtgtcc
    15241 gtatcgatca gttgcaggac tggaccaaac ttccgcttcc cggtgcccct accttttccg
    15301 gaacggcttc ctactctatt accttcaaat tgccctccaa aagatatgat cattacttgc
    15361 ttgacctggg tacggtcttt gaaaccgctg aaataaaact gaatgacagc gtcctggcca
    15421 ccaccatcgg gccggtacac cgggtcctct tcccctcctc ccttcttaaa aggaccaata
    15481 ctctggaaat cctggttacc aacagcatgg caaaccggat cattgacctg gaaaaaagag
    15541 gcgtgccctg gaaaaagttt tacaatatca acttcccggc ccgtttagcc gaaaaccggg
    15601 gaccggacgg cctgtttacc gccatccact gggatccgcg cccttccggg ctcctgggcc
    15661 cggtgaccct gaaacctttt aataaagcga aggaataact cagtccgtaa cctcggcaca
    15721 ccccatccct ttacttatat atttgataga ataatctgac aatgcagcta aataaaatcc
    15781 agcacatcgg catacccgtt accgaccttg gaaaatccgt tcccttttat cagcgactgg
    15841 gatttgaagc ggcgatggaa tccccctttg attaccaggg ggagcgcggc acctgcgtca
    15901 tgatgaaaca aggggaaatc ctgattgagt tgtatcagtt tcctgctcgt tttttacctg
    15961 aaatcagaaa cagaaaggac ggacctgtag atcatattgc ttttgatgta caggacattg
    16021 acctggtctt ttcaggcatg aaagcccggg ggtttaatcc aatagaagag agccccgtat
    16081 ttttgccttt ctggaaaaac ggctgccgtt atttttatat tttgggcccg gatggggagc
    16141 ggcttgagtt taatcagatt ttataaggtc aatggaatca atatttgatc ggatggtctc
    16201 ccgaaaatcc ttatcctaat gttttgcatg gaaaagtggt atcctttttg gagaatatcg
    16261 gtcctttccc tcgtcgtctc cagctgtgtt tacgacagcg ccgtcattcc cacggattgc
    16321 accaatatcc actggaccta tacgatgacg gcaggcggac aggatcattg ggctgatctt
    16381 tgcacaggat acaacgcctg cggcggaatg agtcagtcgc cggtcaatat tactggtgca
    16441 gtggcccagc caaacctttc aaaattaact ttcggctatt caaccaccgc cgccgaaata
    16501 gaaaacaacg ggcataccat cgagtttgtc tgtgatcccg gcaataaatt aactattggt
    16561 ggaacccaat atgaattatt gcaatttcat tatcatgcac agagcgaaca ccaggtggat
    16621 ggaaaaaaat ctcccctgga agttcatttc gtgcacaagg ccaccgattc agactttgcg
    16681 gtgatcggcg tgttatttga agaaggggct tccaattcct tatttaacca atatttgtcc
    16741 cgcttccctg cggaagaggg aacctacacc gatcattcag aaatcgaatt gacggaactt
    16801 ctgccattga ataaaagcta ttatcactac agtggatcac tgaccacgcc tccttgttct
    16861 gaagtagtgc attggtatgt gttaaaaaat aaaattaccg cgtccgcaag ccagcttgcc
    16921 cagttccagg ctttattaaa aaacaattac cggaatattc agaacctgaa cggcagaaag
    16981 atttatagct ttgatgaata gcgtttaatt cacctttaag tttttcttat ggaactgtcc
    17041 aaaaaagaca aaaaaacctg ccgggaactt attgagaccg gtcttcagcg cgaattcacc
    17101 accggattgg aaaaagccga atcaatattg tgcaactgga aagccaaaaa taaagacgta
    17161 aaagaaacct atcatgctct atatagtatg atccgaaaat ttgataacca tatcggcaga
    17221 agatataatg gcatcaccgg gtcgcactat ttatcctgtg tagcaggcct ggttatagat
    17281 caaatcatta ctctggagga actcagcccc ttttcggatg aggcccgcgc caccattttg
    17341 agcatgtcga gcattaaatg gttataaata taattccaat gttttctttt tcttttttta
    17401 agttattctt ccggcccgaa tccggcatgg ccgttttttt aaccgcttta ttgaactggc
    17461 ctctttctgt ccaggcacag tatccgggca tcgccgacga catcaaccgc caccggaaag
    17521 gggttttaaa agtccgggcc aatccggggg acatggtcac ggtggaacaa ctgcgtcacg
    17581 acttctggtt tggctgcgcc ctcagcaacg gttttggcgg cggcactatg aaagaggagg
    17641 accataaaca atatgaagaa aaattccttc aaaatttcaa ttccgccgtt acggaaaatg
    17701 cggtcaaatg gcccagcatg gaaccccgta ggagcgaggt caattataag gtggtagatc
    17761 agttgctgca atggaccgaa aaacaccaga tccccctccg tgcacataat cttttctggg
    17821 gcataccgcg ctttgtccaa gactgggtta aggaactgga tgaccaaagc ctgcttcaaa
    17881 ccctcgaaaa ccgcgcagag acggtcacca gacggtacaa gggccgcttt gccgaatacg
    17941 acctcaacaa tgaaatgata cacggcaatt attatgaaga ccgccttggc cccgatatca
    18001 ccaggctcat ggcccaatgg gcccaccagg gcgacccgga agccaaactg tatttgaacg
    18061 attacgatat cctcaccgga aataaactgg cggagtacct gtcccagatc cggaaattac
    18121 tcgcacaagg ggcgcccatt gccggaattg gcgtccaggg acatctccat tcggatacct
    18181 tcgaccggaa tcagcttaaa aacgccctgg attccctggg cttattcgga ctgccggtcc
    18241 ggattacgga gtttaacatg cccggacagc gttcgaaata ccatatcgaa aaaatcacgg
    18301 agatgagccc ggaggaagaa gcccttcggg ccagggaaat ggtagactat tacaaaatct
    18361 gttttgcgca tccggccgtc gaggggatcc tgatgtgggg attctgggcc ggggccaatt
    18421 ggattccctc ctcctccctg tataagcgcg actggtcacc cactccggcg gctcaggcat
    18481 accaagattt gatttataag gaatggtgga ccaaagagtc cgggaccgct ggaagcgaag
    18541 ggctgttttc ggttccggct ttttatggaa aatataaagt gaccgtaggc ggcctttcaa
    18601 aagaaatcca tttgacaaaa ggaaagggac aaattgtggt ggattttaaa aaataatacg
    18661 tccaatcggt cttattaaaa atgtctgact tgaaaaagat tgaaaaaccg gaacccgggg
    18721 agtacccatt ttatgcgtcc atgtatatcg accttttgcc ggacgatggc ctgttattaa
    18781 aacatcttca agaaaatctt aacctggtta agcagatggt ctccgcgata cctggagaaa
    18841 aatggacgta ccggtatgcg ccggagaagt ggaccataaa agaaagcctg gtccacatca
    18901 tggatgatga acgcatttac gcctaccggg ccttgtgttt tgccagaaat gaaaagattc
    18961 ctttgccggg ctttgaccag gatgcatacg ttttgttatc caatgccaat gaaaggagca
    19021 tagacaatat catggaggaa tacgaagccg ttcgcctggc gaccatagcc ttatttaatg
    19081 gctttgacga ggccgccctg atgcggatgg gaacggcaaa taaaaataac gcaacggtcc
    19141 gggccctggg ttatcatatc gccggacacg agttgcatca tataaatatc attaaagaaa
    19201 agtacctgta gcaggcgaat ttattcgcct tgggcgattg tagcgggcga ataaattcgt
    19261 atgttacatc aactacatac ccgacctggg cgaattaatt cgcccgctac aggtccacca
    19321 cgcatctgaa accgaccgtt gcacatcggt ccagtcccgg ataagtcaaa aggtacttgg
    19381 ccccaaattt tgttttttgc gctccctggt cggcatacca atgtgaattc ctgtttacat
    19441 acccggcgcc gccccgcaat atgcaataat tattgtatcc atccgtgcgt tcgctttccg
    19501 tccattgcca gacatttcca ctcatatcat aaagaccgga ttgggttctt cccttttcat
    19561 actgtttcac ggggctgagg cccccgacct gaccgtgatt gcatacggtg gaatcaaatt
    19621 catttcccca gggataggtg gtttcccggg ctccgttttg ggctgcccat tgccactcgg
    19681 cttcggtggg caatcgtttt cgcgcccacc gggcataggc tcgtgcatca tccaggctga
    19741 cccacactac gggatgtgct tccaggccct ccgggatctt gttgcctttc caatgtttca
    19801 agaaattttc gggatgggcc ggtttatatc cggaatcttc caggaatcgt ttaaactggg
    19861 cattggtcac cgtggtttca tccatggcat aagaattgag catccctttt accggtgcag
    19921 acaagatttg gttgatggcc cggctgtaag agaagtcttc gatgcccacc agcgggtaaa
    19981 aaccgcattc cctttgtcgg aattggaatt ccagaattac cggatcacgg gataccggga
    20041 tctccaccat gccttggggt aatttatttt tcaaatattt gacggtgttt ggcactggtt
    20101 taagatggtg gccgggaatg gaatatgccg tactgaaatc ggaattttta tcctttgagg
    20161 cctggctttg caggaaatta ctaaaatccg cggtcctttc gttttccggg atggatagaa
    20221 tcccgctgat cgctttgggt cttaattcga taattaaaaa agtttttccg ccagccgttt
    20281 ttgttttcac ctcttttccg gtgatcaggt cataatattt catacccggt ataaaatcat
    20341 ctgccaggac cgtccctttg gcccattgtt cctggcgatt gatcaaggtc cagagttttt
    20401 tgccttccag ctcccattga cttgcataga cccggttgag ccatacggga accaggggag
    20461 tccaccggcc ctcggtaaaa aaccgggagt atcggcgctg tgccggaagc attgcatcca
    20521 gaagagaccg gtcccgggga ttcaattcat tgaccgtgcc gaatacgttt tcccaaatca
    20581 cgattccaca gccgttgatc caggcattct gaatcagcgt ggattgttcg tggctgtgcc
    20641 tcgacagcct gtaaatcata tgcctgggtt cgagccaacg gttgcgaacg agggtcggaa
    20701 cttcattgaa ttccagcgca tggtttctcg tccaggccat ttccagccag gactgatgga
    20761 cctggtccag ggcctccggg acgatgggga tctccgactg aaatacagcg ccggggcttg
    20821 aagttttcag tacctgaaac aggctttcaa aattggaaat ggtatccaga aaaaagccgt
    20881 ccgctccaat ttctttcaac aggctcaata attcatccac atcgttcctg cccgccaacc
    20941 gggctgtgtg gtcccatgga ttataagcga tcatcagttt tttacccatg gcatggagct
    21001 cctcgcacaa agctttcagg gcctttaccc ctcccgggag gttcctgtaa aaaaaatact
    21061 ggtccctttc gtcaaaaccc agctggggat acgtgggcca gaggatcacc acatccacgc
    21121 ccccgtatcc ttcctcatat ttttccagat attttttgat tgcaaagttc ccgtatgggt
    21181 catacaggtt tttatcgttg gccataagga ataaggtgga ataggctttc gaagcccatt
    21241 ggtattccgg ctttcggtat atttctcccc ggtacgccat ccggtgcagg accgagtctt
    21301 tccaatcgct gatctccctg cgccaattct cccattgatc cgcaacctgg ggcgcttcga
    21361 tcagctgatc atacctgttt tcgattatac cctgcccggg gacctgggcg catgcaacct
    21421 gaatatagaa tacggagaac aggagaggaa tgattttttt tgaaaacata gtcattgatt
    21481 caggtaaaga acgggcttgg ctgttttcat caagaccttc tgtcctcttg cgcaaaaatt
    21541 tgtttaaatc taactatttt gtttgtccaa tcgtgttcag taaatgaata cgttgtcttt
    21601 ctttcaatta aactgttgaa tatgaaaaaa aacaaaattt ctggcaatga tcgcaggact
    21661 tttataaaat cgaccggggc agccataatg ggcggtactg tggcctttaa cctgggcctg
    21721 ccgggacagg cctttgcccg gaaaacgttc aaaacggcaa atactctgaa agtaggcctt
    21781 atcggctgcg ggggaagggg cacgggcgcc gcggcacagg ctttggcagc cgatccggat
    21841 gtggtgctta ccgcgatggg ggatgttttc ctggaccgac ttgaagaatc ctacaaatcc
    21901 ctcgtggaag cggctgccca aaaagtaaag gtggatccct ctcataaatt tgttggtttt
    21961 gacgcttata aaaaagtcat cgactcgggg gtggatgtgg tcatcctgac gacccctcct
    22021 gccttccgac cggatcatct gatcgccgcc atcgatgcgg gaaaacacgt tttttgtgaa
    22081 aagccggtcg cggtcgacgc ccccgggatt cgcaaggtgc tggccgcggc gaaaaaagct
    22141 tcggataaaa aattgtctct cgtatcgggt ttcaccttcc gttatgagta tgcaaaacgg
    22201 gccttttttg agcggatcac gcagggtgaa atcggcgaag tacgcactgt atcctccacc
    22261 cgcaacggtg gttatctctg gtataaacca cgccaggaaa actggacgga catggagtat
    22321 caaatgcgca actggtatta ccaaaactgg ttgtcgggag attatctcgt ggagatgatc
    22381 gttcacagcc tggatatgat gtcctgggcc gtcggcgata aactgcccct gcaggccacc
    22441 gggaccgggg ggaggcaggc ccgggttgat aaaatgtacg gaaacattta tgaccacttt
    22501 gccattgaat acgaatatgg caacggaatc aggggcttca gtttcagccg tcaacaagcc
    22561 ggttgttcca cgcgcaattc ggtggaaatt gcgggttccc tgggtcatgc cgtggtgaaa
    22621 agcacgggga tccctgaaat caacggcaaa acccggtgga cgtatcaggg agaaaaaaac
    22681 gatccctacc agacccagca caatgaactt ttcgcctcta tccggaacgg caaacccatg
    22741 aatgacggtc aaatgatggc caacagcacc atggtggcca ttctgggaag gatggtggcc
    22801 tatagcggac aaacgatcag ctgggaggac gccctccaat cgaatcaggt gctcggcccc
    22861 tccatagatc agtttaactg ggatttgaaa tttgccggac cggaagtggc catccccggc
    22921 attaccaagg ttttgggctg acccgcattc atataaaaca agaacctctt ggcgcgattt
    22981 attaaaccct gttgctggct gttcctggtg agcaacttcc aatattcggt tgcgcaacct
    23041 tcaaccctgt cttttggaaa agaggtctct tccatccagg agaaaagaaa aggaacaacc
    23101 ataaactgga tcaacgtcaa cacggatccc ggtacctgga ctttgaaaaa gaatgaactg
    23161 gtttgttcgg gaaggcctat cggggtgatg cgaagcgaaa aacaatacga aaattttatc
    23221 ctccacgtgg aatggaaaca tatggaaccc gggggaaatt cggggatttt cgtctggagt
    23281 gacgccatcc cgggagaaaa gaaccgtctt cccaatggag tggaagtcca aatgcttgaa
    23341 ctggattggg tcaaactgca taccaaagac ggcgtcgaac cccccattgc ttatgtgcac
    23401 ggcgaacttt ttggagtcgg gggcgtggaa accgttccgg acaacccaag aaatacccgg
    23461 agcaagtcca tcgaaaaccg gtgcaggggc cgtggtgaat ggaataccta cgacgtggtc
    23521 tgtgtcgatg gagtggtgaa gctgtctgta aacggcaaat ttgtaaacgg tcttgcaaaa
    23581 gccacccaaa agaagggcta tctatgcctg gaatcggagg gggcggaaat ccactttcgg
    23641 aatttttacc tgatggagtt gccgcccgga gttacttcag cgtctcaggc tgcgccagag
    23701 ataaactgac caactagcta actggcaaac tgactgactg accaactaat tacctataaa
    23761 cctccctcaa ttccagcgtc ccctgataat tcagatgcgg acaggttttt gcaatggcca
    23821 gggcctcgtc tgcattatcc gcttcaaaag taaaatatcc tcccagctcc tgtcttccca
    23881 tgaattccgg tcttgcctca acccgatttt cccgattttt cccggataag atccaaccac
    23941 tgctatgcaa gtgctccccc ccggcaattc ttttcgcctg gatgtcctcc aaccaggccc
    24001 tgtattccct gacctgttgc atgggatcct gggccggaag gtcgtcatta tgtaccagga
    24061 gtatgaattt cgaggctttg ggcgcaggca tcgccattga atcccgcgat ccgtccggtt
    24121 tgcctgtaaa gtatcccgcg gcaaataatc cgatacccag caagagggat gcggcaaggc
    24181 ctgaccatga cctggtcaac agtggtttgg gttccagaat gagattttcc tgtttcattt
    24241 ttttgatgat tttggtaatt aaataagcag ggacccgctc atggggtgta tggatttgga
    24301 gtcttttttt ttcttccggc gtcaatggat tttccatcgg tttgaattaa atgttttcct
    24361 tttgaataag gttgcggaaa gctcttctcg cctggtgcag ctggcttttg ctggttcccg
    24421 gtgcaatgtc cagcatccgg gctatctctt catgtttgta tccttccagg tcgtgcaaca
    24481 ggaggaccgt ccggtacccc tcgggcaaca aggccatcaa gggttggggg tcgtttattt
    24541 ttattgtatg cgcgtgcgac tgcatatggc tttcttccag cggcacttct gatttttttc
    24601 tattcatttc cctccaccgg ttgatcagga ttttaatcat ccaggtacgg agactggatt
    24661 tccattcaaa cgaggacagg ccgatgatcg tcctgagcca ggtatcctgg accatttctt
    24721 cggcctggga attattgccg gctgccagtt tcatagccat attccacagg gcctgggcat
    24781 gcctggaata caacaaacgg aaagcggtct catcccgggt cctgagatag gtcgtcacca
    24841 gcgcctgatc aataaaattc atgacttgta aaaatcaata tccttctttc tcccagacca
    24901 ccggttttgg accggttttt tgaagcgggc atcacccatt agtgcgctga agtggaaaaa
    24961 ggttgtatga cttgtatttt ttttatccga attccggacc gaggattgcg tattgttttt
    25021 ccggttcgcc tggaaaacgg tttgaccccc gatacattct gtaaaacggg cgttgttctt
    25081 caaagccaat agactttaac caaactggcc aggaggggtc aaaatgcggt acatccaaaa
    25141 gaagagaccg gcctctgccc tggtttattg ccgccagcac aaggtgccgg gcggtctctg
    25201 catcggcggc caccacggga ccaatatgaa aataccggtg ccccggccgg ccaaaacaat
    25261 accccaaaag tttattgttt tttttcagaa caaaagccat ttccggagcc catgtccaaa
    25321 tccattcaag caaagaaagg cgatcggcgc caaaaattgg actgtccgcc tcctttatgg
    25381 ctccccattc attcttttga aaaacctttg cggcgctgat ataaggggag tggttcaccc
    25441 ccggtttgat catccggctc aaaggatatt cgtccacaaa acccatttta agataaagtt
    25501 gccgcccttc gggagtggca tccagtttag ccgtagtttc tttatttatc agatccaggg
    25561 cctttcgcag caattccatg cctatgcccc gacctctata ttccctgtcc accaagacca
    25621 ttccaatcca gctgaaacga tccccgtaag gcagggtgac cgctgtgccg atgaccttgc
    25681 cctcttccat cgccaggcaa gcccctggac tgagcttcag gaaaatttgc cagtcccttt
    25741 caagctggtt ccatcccgcc tgcctgcata agtccaatcc ccgcggtatg tccgcttccg
    25801 tcattggacg gcaggccatc atgctttatt cattttcttt ggtaaaaagg caatcgccag
    25861 ggctccaatg gcagccagtg caataagcgc ggcaaaacca ttggcataac ttccgcttat
    25921 atctcttagc caacccacca gcataggggc aaaggcttcg cccaaaccat ccatagtgag
    25981 gataagcccc atgacccggc ccataaccct taccccaaac aattcagcgg ccatgagcgg
    26041 aatgatcata taatcccccc caagaccaat cccaaacagg atagcaaaca gatagacgac
    26101 acccggggtg gatacagaat acaataaggg aatggagccg gccaccaaac aatagatcaa
    26161 taccatgacg tattttttag ggatgcggtc ggccagccag cccataaaca agcgtccaaa
    26221 tatgctggat gccaggatca gtgaaatgat atttgcagct tctccctgtg aatatttcag
    26281 gtccaggctg aaaaagagtt tgaggttttg actggtgccg ctcactgccc cgatggaaca
    26341 catactgccg atcaccaaca ggtaaaaagg ccaggacttc agcactccgg ccaaatggat
    26401 cttttccgtt tccgtttttt tttcctgtag gttgacttcc ggattttctt tgacaaacca
    26461 ggccatggga aaggcaatgg caatcatggc catgccgagg atcattaaag cgagatgcca
    26521 tccaaaatgg acattcagcc accgggcggc ctgggggacc agcattcctc caatgccaat
    26581 gcccagataa gcgatgccca tggcctttcc cctggattta ctgaaccaac gagagatcaa
    26641 aacctggttg ggcaggggcc cgccgcacat gtatcccagg gcattaaaca ggtaaaaaac
    26701 ataaaaatgc cagagctggg tcatttggct caggccgatc acagcaatac ctcccatcag
    26761 gatcccggcc agcatcaacc ggcggggacc gtagcggtca ataaaccatc cggcaaaaaa
    26821 gcccaacaaa ccgacaaaga ccttggcgaa agcatttcct gaagtaaccg tcgcccgggt
    26881 ccatccgaat tcctgcaccc agaaatcgta aaaaaagggc aggccgtaaa acatgatccc
    26941 gaccagggaa aacaggctta aaaagccggt cgcacccacc ataaaatgcc cggggcttaa
    27001 tttatcttct ttcattatgc ttggttctgc cccaggtcag cttttcaggt gtttatcaaa
    27061 ccagacaaat gcttctttct gcatcgcggc gtcgaattta tgcggccccg ggtaatagga
    27121 acattcaaac tgtcgggcgg cctccgcttt gccatacact tctttcagaa tctgctccgc
    27181 ctttttcatt tcatccatgg tatacaaggc gtcgtccaca tcattcagaa ccagggtggg
    27241 caggggagcc ctcagtccaa ggatctccgg aaattccagt tcattgggca gcaggggcac
    27301 ataggtcatc caggtatggg tataagattt attgagaagg aagtctttcc aggtagacat
    27361 aaaaccaacg caaaccgcac accggatccg ttcgtccagg ccggccataa ataccgtccg
    27421 caggccgccc cccgatagtc cgccgcatcc aagtcggttt gcatccacgt cactgcgcgc
    27481 cgccaggaca tccagggcta cccggtcttc tgcaaaaaat acccccggcc aggtggtccc
    27541 ggcgcagaac agggacttcg ccagaatatg ttcatgttgt ccggcccatt cattgtatgc
    27601 ttttattttc tcaggatttt ccggatcctc gtcagacagt ccgcgtctct gagaaaccgg
    27661 tacatcatgc atcatcaccc ggcggcttcc aaatacaaag gcgtccggta ccaaaaccgt
    27721 gtatcctttt ttggcgattt cattggccca ggcaaatccg ctgtaataat cccgttgatg
    27781 ggacaccatc aggggatgct gttggtcaga agttttggtg attttccggg taccaaaata
    27841 tttgtttccc ccatgatcgt gaaaagcaag gatgccaggc aggggtcctt ttgcattcgc
    27901 cggcttcagc aaaaccgctt cagtaggcct tccgtagggc aatggccaac tcagttcttc
    27961 aatgtacaag ccatcgtata catattgttt gtgtaccgtt accctcgggg tgcttcccag
    28021 ctccggaatg gccattcttt ccttaagcct ttgccgcgcc gattttcgcc aagcctccgg
    28081 atcggtccat tccgggtttc ggaatgaaaa ggatggtcgc tttccttcca tccttttagc
    28141 agcccagggc ccgtactggc cgataatgct cagggattca ttttgccatt cattaaatcc
    28201 gggataaacc gcattcaacc gatcgaagga ttccaaatcc ctccaacctt ttaaacccaa
    28261 tcccattccg gccaatccag aatatttaat aaaatccctg cgtttgtttt tcatttcgaa
    28321 acgataaaag gaaatgaatc aaatttaatt caatcccgga agtctggata catcctgatg
    28381 atccgttcta tttcttcccc ttgtcccggt gagagggtct cctccgggtt aaggcagtgg
    28441 accccttcca ggagcccctg cctcctcagt atttcgtgga tgccgggtat acatccctta
    28501 aaattattct ttgcatcaaa aaccacagca ttgatatccg tgaccgcgat ccctttggcc
    28561 aataagcgtt ccgtccctgc gtagccttgg ccgatgcagg ttttgatttc tttcaacagc
    28621 tccaccgctt tgccggtcca tactgcccag tggcccaata agccccccac aaatcttttg
    28681 gtcacctgcc ttccggacac cataaattga tatggggtca gcaagtctgc cacgatattg
    28741 tcgtcgttgc cggtatacag ggcaatttga tccttcctgg acgattcaca gaccgcacgc
    28801 accacatcca gggtctggta ccggttgaat gcggccactt taatggcatg taccttggga
    28861 atctctgaaa aggcaagcca gaaatcatag gacaataccc gtccccctac cgcaggttgc
    28921 aaataaaaac caaaaacggg aaggacctcc gaaaccgctt ttgctctttc gagcagttca
    28981 ttttccgaat aagcagccag acctcccggg ctcaataatc caaaatgata accatgcctt
    29041 tttgccagct ccgcttcttt tatcgcctgc tcaacgggac cgcagatccc ggccactttg
    29101 ataaaaggcc tgtttaaggc ggcttgatcg acttcctcgg ccgcgagtcg caatacgggt
    29161 tccagcaaac cgatacccgg attcctgatt tcaaactggg tagtatgcac cccaaccgct
    29221 atcccacccg ctccggcttc catataataa cgggtaagcc ccctttgccg ggcttcatcc
    29281 agtttaagcc tgctgtccag ggccagcgga tgggccggga tcaccgttcc ttcatgcaat
    29341 aagccgaata cctcctcctt catcccttaa ttcttaattc ttaattcttt aattacctaa
    29401 aacttccctt tcctctcctg gaaatgggtc tccttgttta tggtcttccc cccttcctca
    29461 atccaacgcg ccagcagccc gatcatttgt tttaagccca ccctgggata cccaaacaac
    29521 cggaaacatt cggcagcatt gctcaacagg gctgtatcct gctcttctcc tgtaaaaatg
    29581 ggtggctctc cgaacaagct gccgaattca ttggcgatcc agcgcaccga agcggtttca
    29641 ggtccggtga cattgagcaa tttgcacgga acggcacaat gaggcagcgc gcgcagggcg
    29701 atctcattgg catctccctg ccagatcaca ttcacatggc ccatgttgag gtcaatgggt
    29761 tttcgggact ttacggactt gccgatttca agcagaaccc cataacttac atcattggca
    29821 taattgagcc ggtagattaa gatgggtgtc cggtgcagaa tcgaaaaata ctggaacaac
    29881 ctttcccttc ccaggcagga ctgtccgtaa tctcccaaag gttccggctg aatggattca
    29941 tgcgctccgc cgctttttac ggaaacgaag ggatatacgt tgccggttga aaacacgaca
    30001 atcctggaat ctttaaactt ttcagctacc cttcccggca aataggcatt catggcccag
    30061 gtaaaggatt cattgccctg ggtgccgaat ttagtcccgg ccaggtacag cacgtttttg
    30121 accgttggca gggcctgaag ctgatcatcc cgtaacaaat ccgcccggag ggtttcaatc
    30181 ccttcccggt ttaattcctt ttccagcccg gcttccgaaa accgcgcaac accgattact
    30241 tttttattta ttcctgccgc atcaatggcc tgcttcgctt tccgggccag ggccgggccc
    30301 atctttcccc cggcgccaag aatcaatata tcgccatcca ggctgcttat atcggcaatc
    30361 agttccgaag aaggcctcag cagtttttga tatatttccc cgatcttttc caaaaaaatg
    30421 ttattaacat taaatcgtat aaatctaaat actttctcgc agggtggtat caggtgcccg
    30481 aaaatgaatt tattcattca ctcctctttc ctgattaccc cggctcaaat ctgctaacta
    30541 aatccactct atctttgtcc ccttatcaat cctcaatcct caatcctcaa tcctcaatcc
    30601 tcaatcctca atcctcaatc ctataaatcc tataatccta tatgcagtgg atcgactacg
    30661 taggcctttt cggcgccttt ctctcctccg tcacctttgt tccccaggtg tataaggcct
    30721 ggcaaacccg gtcgaccgga gacctttctt attggatgct tttgatttta ctggggaatg
    30781 tatcgacctg gcttttttat ggcattgtca aaaaggacct ggccatcatc attgccaacg
    30841 ccattatcct ttccctggct ttgatcctcc tgtattttaa aatcaccttt aagtccaaac
    30901 cctcttaatt cccttaattc tcttaatttc tctaattact ttgccctccg catcctatcc
    30961 cccttcccca gtaacactca ccggcgaaca ggtcagcctg atccccctgg gagaaaccca
    31021 tgaaaacgac ctgatcgaag ccacccggga cggcgaattg tggaggctgt ggtacacctc
    31081 cgttccctcc cccgaaaccg taaaatccta tatcgaaact gccctgaatg aacaggccct
    31141 gggcaagagc cttccctttg cggtcctgcg gcatgccgat aaaaaaatag tgggcaccac
    31201 acgttatatg aacatcgagc cggggattcg ccgcctggag atcggttcca cctggtatgc
    31261 cacccacgcc cagcgtacgg cggtcaatac cgaatgcaaa taccttcttt tgcaatatgc
    31321 ctttgagtcc ctggaatgta ttgccgtcga attcaggact caccggttca acgaagcatc
    31381 ccgccgcgcc attcagcgtt taggcgccca gcaggacggg attttacgaa accaccgcct
    31441 ccagccggac ggcaccctcc gggataccgt ggtctacagt attctcatcc aggaatggcc
    31501 ggtggtcaaa agtcatttgc ggtttaaatt ggcgggcggc gggcgctgag ctgttgcata
    31561 acgaggtttg ctttaacccc tggtcggata cccttttccc aacatgcctt ttcacagggt
    31621 cctccccatc aattccagat ttgtaatttt ttacaaaaat ggagtctttg tccagttttt
    31681 gactattttt acaaaaatgg agtctttgat tgaccgtcaa atattgccgg aagcccgcaa
    31741 ggccttgcgc cagtttaggg cactttgcat caccggcccc agacaaagcg gtaaaaccac
    31801 cctgagtaaa caactgttta agggaaaaac ctatatcaat tttgaaaacc caagcacgca
    31861 gtttgaagcc gagctaaatc ctgatcggtt tttgaaacaa tatctttccg gtgcgatcct
    31921 ggatgaagtt caacgtgtac ctcatatatt cagatatctc caggaaatcc ttgataataa
    31981 tccgtctcgc ggtcagttta tcttgaccgg ttctaataat tttttattgc atgaacaagt
    32041 atctcaatct cttgccgggc gggcgggtta tctttctctg cttccattca gttatgccga
    32101 attaaaggcc tcaaacttgg cttattctga tgtacaccgg ttaatcttaa ctggcggata
    32161 tcctgagatt tggcaggagg gcctcaatcc ggggcagtgg ctgaatagtt acgtacaaac
    32221 ttatgtgcaa agggatgtaa ggcttatccg taatatatca aaccttgcag tttttacccg
    32281 ctttcttcac ttatgtgcaa atcatgccgg tcaaatactc aaccgggaag agcttgcgcg
    32341 tcaaaccggc gtagacacga aaaccattct tgcctggttg ggattgctgg aaaatagtta
    32401 tatcctcttt ttacttcaac cctggtacca aaatttaaac aaacggatcg tgaaaagccc
    32461 taaactgtat ttttatgaca ccggcctgct ttgtcattta ttggggatta aaaatgtcca
    32521 ggcccttacc aggcatcctg catgtggtgc gctatttgaa aactggatca taactgaaat
    32581 tcgaaaaaat acatttaata caggcagtca acaaggcatg tattttttca gggacaatgt
    32641 gggaaacgaa gtggatttga tactggaccg ggaggagggt ccattagcaa ttgaaatcaa
    32701 atcatcgact aaggcagaag gcgccatgtt gagaaatctg agattttggt taaaaaatac
    32761 gcgtgacggc cgggccctcc tgatctatcg gggcaaaaaa aacgaattca ctgaagatta
    32821 tattggcatt gctccttgga cagaagtatc ggacctttct taaattccgg caatccttca
    32881 atttcaatgg tgtgattgag cttattttat gccctccatg atctgacaaa ccgtttccag
    32941 gcttctttca tcacccacat tgcccggaaa cacgatataa tacatcccgg gaaacctgct
    33001 ttcgggaccc aattgccaga ccggaacccc ggggatcacc ggccccagga tcctggcgct
    33061 ttttaccagg agcccctttg aagcgatatc gctggaggta atgccgcctt tagccagtat
    33121 ataccggggc cttaccgcaa gtcctttcaa aagagatacc aggaaatccg acacttgggc
    33181 gttgatgtgc aggttgctta tatcgtcgtg tccttcctgt aaaagccggc tggtatacac
    33241 taaaactttt ttatcggcgg ccagccattg attgatttga ccggtgatgg gttccggctt
    33301 ttccaggccc ttcaaaacct tcaccacatc aacctctatg gccttatgcg tattccttgc
    33361 cagcaaggca ttaagttgtt ggcttgtttt cgacacataa gacccggcca cgaccagcgt
    33421 accactgccc ccggcttgtt gaagggggga tggcttaaag ggcctgccgg attccatgcc
    33481 ggcgcgaatg ggcacaaagg tggctgagct gcggaataaa atcgttttcc cgttttgacc
    33541 tgccaagatg gaagccaggg cgacgacttc caggtcccgg taaccggcgg cattgatgat
    33601 caccgttttt ccttcccggc agtccatgaa tattcccgcc actttctccg gtccgcccgt
    33661 gcgaatatcc tccagggata ccgaaaggac ctccgcagcc cggacctttc cttttgattt
    33721 ttcttccacc caattcttca ggttcgaatg tttatacccg aagaccctgt cgcgggcaaa
    33781 tgcagtttct gaaaccggga ccagttgctg gttttccgcc aggtagtgca cttcttcaat
    33841 ggtataccgg cctccttcga taaaggcggg caccaatacc cggatggcct gttgcaagcc
    33901 catagcttca acaagggcat ctgtttctgc aggaaaatgg cccctgagcg tggaatcact
    33961 ccggctgatc aacacgattt cccgcccgct tttttggatg gcaatattca gattccggcc
    34021 aatatcctgg cttagtttta ttgcatcctc ctccggaagg cttcttgaat tggtcagaat
    34081 aaagagcacg gaaggttttt tatccaattc cgccacgatt tcttccggcc gccatgaggt
    34141 gatgacggtg gcctcgctgc tcgtctgcgt gccggtcggg tcatcgtcga gcaccaggat
    34201 ggtctttccg ctttttaaaa attctgaacg gatggccgga aggaggggcc ggggatattc
    34261 cgggggcaag gttttcagtt tttccttcag gtcctggtac atgaccggca aacataaaga
    34321 attattttat cggggattca gttactcttc caagccaaat taatttggcc gctacacctt
    34381 atcttcaaac tcatgcagat catcatcacc gggggagccg gttttcttgg ccagcgcctg
    34441 gccaaagcct tcacggacgg aaactttgat tttaaatcgc ttcagctggt agaccaggtt
    34501 atgccggcaa acccggcaaa cgaccatcgc atagaatgcc gccaggcaga tcttaccgaa
    34561 gacggggtca taaaaaccat cctgaaaccg gataccagcc ttctcttcca tctcgccgca
    34621 gtagtgagca gccatgccga aagggatttt gaccttggat ggaaagtcaa cctggacctc
    34681 acccgtcaac tccttgaggc ctgccgccat cacaatccct cgattcgttt tgtgtttgca
    34741 agctccctcg cggtctatgg gggcttgctt cccgagaccg tcggggacac caccgccctc
    34801 acccctttgt cttcttacgg atcacaaaaa gccatgggtg aactgctcgt caatgattat
    34861 tcccgtaaaa aatttgtaga cggcagagta ttgcgcctgc ctaccgtctg tatacgtccc
    34921 ggccggccca accaggctgc ttcttctttt gtaagcagca tcatccgcga accgctgaat
    34981 ggcgaagagg ccctctgccc cgtatcccct gaacttgcat tatggttatc cagtccggac
    35041 accgtcattc aaaacatcat ccacggcgcc ttgctggatg cttccgcatt cggagcctgg
    35101 cgctccgtaa acctgccggg cctgcgtgtc acggtaaagg acatgctgga tgccctgggc
    35161 aaagtcgcag gcagaaacac cctggataaa attcaattta agcccgatcc cgccattaat
    35221 aatattgtga gcagctggcc gggccgtata gacaacgatc ctgccttaaa actcggtttt
    35281 caggcggacc gtctttttga agattttatc cgtcagtacc tccaatccta tcaatcccgg
    35341 ccctgatgtc ctctttttct tttcctattg tcggcctggt cgttgcggtt tttgtattga
    35401 tctttcttgt gttgcgcacc agggtgcatg ccctgatcgc catgatcctg gctgcctgta
    35461 ttgccgggtt taccggtggg ctttcggtca ccgccaccct tgacgccatt acccgtggat
    35521 ttggttccac cctgggcacc atcggaatcg tgatcggcct gggtgtaatg atgggccgta
    35581 tcctggaaat atccggtgca gcggatcaaa tcgctcattt ctttattcaa tggctgggcc
    35641 gccggaaaga agagtgggcc ctagccctca ccggatacct ggtgagcata cccatatttg
    35701 cagactccgc ttttataatt ctgtttccca tttccagcgc cctggccaaa aaggccggcc
    35761 tgtccgtttt atccctcgga gtggcgcttg ccggtggcct tattgcaaca cataccctgg
    35821 tgccgcccac gcccggcccc ctgggtgtgg cgggactttt cggggtggac gtcggggaaa
    35881 tgatcttgtt gggactgtcc ttggccgtac cctgcaccct ttcttttgtg tggtatgcca
    35941 aatggctggc caaaaaatac ccgggattta ttgatcctgc acccacatca gaaatcccgg
    36001 aaccggatta tgagaaacat tttaatgacc ggaccggaaa agcccttccc gggctgaccg
    36061 tctctttgct tcctatcgtg gtgcccatcc ttctgatcat gatcaagtcg gccatttccc
    36121 ttttcccttc attaagcgct gaccaaagcg ccccggtcta ttggattgta actgccttcc
    36181 attttctcgg ttcgccaatc attgccctat gcatcagtac cctgctggct gtttataccc
    36241 tggtacccca tctggatcgc cgccagacgg cgacaaaact ggaagaaggc cttcaatcgg
    36301 ccggaattat cctgatggtc accggcgctg gcggtgccct gggttttgtg gttcgtgaaa
    36361 gtgggaccgg cgcccaaatc gctgatcaaa tctcccgcct gcccttttcc ccggtcatga
    36421 ttcctttttt cgtggcttct gcaatgcgtc tgatccaagg ttcggcaacc gtggctatga
    36481 tcacttcggc ttcgatcacg gctcctattc ttcaacaggt accgggggtg aatatgttgt
    36541 ttgccgccca ggcagccgcg atcggcggct tcctgttcag ttattttaat gacagcctct
    36601 tttgggtggt caaccggatg atcggggtca cggatacccg gcaacagatc atggcctggt
    36661 ccgtgccgac cggcatctcc tgggcggtcg ggggaatttt gatcgcgttg atcaatctga
    36721 tatggggatc gggtggttcc tggcttgatc cggtcatccc gggtctgatc ctggcgattt
    36781 tgctggcgac ggccttttcg accaaatgat gcattaaata acctgtttat atggaaaagt
    36841 ccaaaaccac cctggcggaa aaatatctgt ccctcgattt ttcagaaaaa gacagcatcg
    36901 gtctgaccga acaaaaaacg cttcgtatac tgatcggggt tcttggagtg ttattgccgt
    36961 tattgttgta cctgtccttg tatgttgatt ccgggcatac ggttccgctg gaatccataa
    37021 gccattatta ttacacccgg gccaacagct tttttaccat taccgtgagt ttgctggcgg
    37081 ttttcctgct gatctataaa ggcaaggctg cggtggattt ttatttatcc gccctggccg
    37141 ggatttttgc gcttttcctg gtgctctttc caacagataa tctttccttg gtttgctgtg
    37201 atcaggaaaa accatacgcg gtcacgcttt tgcccgggaa ccccctccgt gagggttttc
    37261 attatttatc cgccgccatt ttcctgacca gtctggccgt tctgtcttat tttatattca
    37321 ccaaatccaa caagccgccc ggcgaaagaa gccctggcaa aatataccga aatcttgttt
    37381 acaggacctg cggcctgttg atggccctgg ccttactggt tgtcctggcg gggactctta
    37441 atctgattca tgagccctat tataccgggc atcacctgac tttttggatg gaaacccttg
    37501 cagtcgaatg tttcggtatt tcatggctga ccaaagcgga agtttttttc aaggactgac
    37561 ccttcctccc ttttccctgg gtttattttt ctatttttaa tgctcatcca aaaccactaa
    37621 aaaaactccg gagaatccgg aattcaagac ttcgctactc atgtctatcc aaaatccaat
    37681 taagcgactg cccctgttga ccgggatatt gctttttatc tgcgcatgcc aaaccaaggc
    37741 cccgcaggac gaccgtcttt acaccggcga cctcttccat gaaaatatcc ggagtaccga
    37801 tccccggacg cctgaagaag aacgccaggg cttcaaactg ccccccggtt tcgagatcca
    37861 gctctttgct tccgaacccg atatcgataa gcccgtcaat atggcctttg atgccaaagg
    37921 acgaatgtgg gtcacccagt cgtttgaata tcctttccct tcacctccgg gtaaaaaaag
    37981 cacggacaaa ctgaccatcc tggaagacac cgaccacgac gggcgggcgg ataaatttac
    38041 cctggtgtcg gacagcctca atattcccat cggcttattg cccctctccg acggacacct
    38101 gtcctttagc gtaccccatt tgtaccgttt taccgatgcg gatggcgatg aaaaacctga
    38161 agggtcacgg attctgctcg gcccctttgg atacgaagat acccatggta tggtgagcaa
    38221 tttcatacgc ggttatgacg gctgggtcca cgcttgtcac ggctttacca atctttccac
    38281 cgtggccgga agggacggag attccatacg cctgcaatcg gggaacacgt tccgtttcag
    38341 gctggacggc agccgggtcg aacaaatgac tttcggacag gtcaatcctt ttggattagc
    38401 ctatgatgaa cgggggtata tctattccac cgacagccat acttcccccc tttaccagct
    38461 gatccgcggc gccgactatc cccatttcag taaaccggaa atcatggctt tcgggcccga
    38521 catgaaacca ttggaaaacg aagccaccgc cctttgcggc attgcttatt atggcgatac
    38581 tcaattcccg gaggaattcc agggtaattt tttcatcggg gacgccctga tctcaagggt
    38641 gcacaggtac agctggacca ataaaggatc tacccctctg ggcaaatccg aaaccgattt
    38701 tatccaaagc gccgatccct ggttccggcc ggtcaacatc aaactcggcc ccgacggcgc
    38761 cctttatgtg gcggactttt acaatgccat catcgggcat tacgaagtac ccctgggcca
    38821 ccctaaaagg gataaacaac gcggtagaat atggaggatt acctacaagg gcaatcacca
    38881 tgaccttcag gacctgactc aagcttccat ccctgacctg ttggccaccc tcgacgcgga
    38941 caatataagc acccggatgg ccgctgccga tcagttaacg gatcgtttcg gcgaagcggc
    39001 ccttgcccag gtccgtactg ttttggatga aaccgggacc cttccccgga aatacatcca
    39061 tgcactctgg gtcacctacc gactgggcgg tatggatgat gcccttctga ataaatccct
    39121 ggttcatgat tcgcccctgg tccgcctgca ctcgctcaga atattgaggg aatggaagcc
    39181 gggagacgct tctttccaat ccatggttgc ccgctcgctc aatgacccgg atgcccatgt
    39241 acagcgggcc gccgctgaat tgctgtcttc ttatccagag atttcatccg tcgaaagcgc
    39301 gctgcaaatc ctgcgcgcag ctcccgcaga agatacccac ctccggtaca cggcgcgtct
    39361 caatctacgt aacttattac gcgtagaatc cttgatgaaa acagtagccg caaaggaatg
    39421 gaatgaacag gatgcaggac atatagccgg caccctggta gatgttcatt cagcggatgc
    39481 cgccctgttc ctttccaatc acctggccag gtatgccttt ccaaaagaaa agatacagtt
    39541 ggcttatcag cagatcatgc gctttttgcc cgcttcaaag ctggatgaag tcattcgcag
    39601 tgcgggtgcc aaacaggccg aggccgacct ggccgcccgg atattcagag gggtcaaaga
    39661 cgggcttgcg caaagagggg gagacgctaa aaccggtctg atgcaatcct ggggcacaga
    39721 gatcgcaaaa ggcctgattc agagataccc ggccgattcc atttcgaggt ccctggcgcc
    39781 gcaacaactg tttgccatcc aacttgcggg tgaatacaaa atccggcccc ttcaaccgga
    39841 actggtttct tttgtggaaa ataacccggg ccatgatctg aaaacggccg ccttatcttc
    39901 tcttatgaaa atgtcgggcg accggagcac cctcgacctt gccagcaaat ggctgatgag
    39961 cgacagcacc cccgtaaccc tcaaaaaaaa tattgccact gtcctgggtg aatcgccggg
    40021 atccgacgta aataaaatcc tgctcggggt caccaatgcg cccgctgatc tggaggtcgt
    40081 ggtcgctaca gcccttgccg gttcgccgga aggcaaagat ctggttctac aacaagtccg
    40141 taacggggct tttaaggcaa gaaccctgat cgatccgagg gtggaagaaa aaatgctctt
    40201 cagaatttcc cctcaacaac aaaaaacgta ttcagagctc accgcccata tcgaacccat
    40261 cagcgaggaa agacagggcc tcattgagga tcggctggaa gcattcaaac tattcaaagc
    40321 ctccccatct caggtggata ccggcgccag gctgtttaat gcaaattgtg gtatttgcca
    40381 ccggcgtcag accgaatccg gcatcggtcc ccagttgcat ggtattggca aacggggcgc
    40441 gaacgccatt gcagaaaaga tcctggaccc caaccgcaac atcacggaag ctttcagaaa
    40501 ctataccatc aaactgaaag acggaaagat cctcaccggc ctgtaccggg gagaacaggg
    40561 agcggtggtg atttttggcg atctcaccgg aaaggagttt tccatccgcc gggcggacat
    40621 agtcgaacag aaaccttccc gctataccat catgcccgac cattttggca ctacccttac
    40681 ccagcatgaa tttaatatgc tactgagtta tgtactgacc tggtagcccc agccaagtgg
    40741 cggtccattt gctcctgcca aggtgatcgc caatcggccg tccttcgcgc aaccccggtg
    40801 ttccccttta atcgtccttt aggggtcgga ttttgcgttc tgtcatacta aattccaact
    40861 tgcattgcca attaactaac ttgcaaattg accaacttgc taactaactt tacttaaact
    40921 atgaaaaaaa atacaggtcc tttgttttta ctgttgattc ttatttattc cttctctccg
    40981 gcccagaccc caggcaaaga ggtccgcttc aaaaaatttc atttatggga cgagttctac
    41041 acggaagggg ctaccgtggc cgatgttaac aaggacgaca ggctggatat catagcaggc
    41101 gcccgctggt ttgaagcgcc tgactggaag gcgcacgata tctggaaaca caaaaaattc
    41161 gattatacca aaggatatag cgactccttc ctcaatttcg ccctggatgt caacgaagac
    41221 ggatgggtgg atctcatttg ccatgatttc cccggcaggg aagtgtattg gttccagaac
    41281 cccggaaatc acgaggtttt gtggaaacgg catctgattg attccgtcgc atccaatgaa
    41341 tctccgatgg cggtggatgt tgacggcaat ggtaagaagg atctggtatt tggcaatgaa
    41401 aaaaccggcc agatgaactg gttcaaggcg gttgtgaaag aagggcgcgt aacctgggtc
    41461 cggacggcca tctcagaacc caaggccaaa ggcgtaggcc tcttctccca tggacttgga
    41521 tggggggatg tcaacggtga tggcctgaac gatgtgatga ttatcggtgg atggtgggaa
    41581 gcccccaacg acataaaggt ttcgccctgg atatttcatg aagccaccct tgggaagccc
    41641 tgtgcgcaga tgatcacctt tgattttgat caggacgggg atgccgatgt ggtttcctcc
    41701 tccgcgcacg attatggctt gtggtggtac gagcagcttg tagagccggg cgccaacggg
    41761 gaccccaaac gtacattcat cacccatacc atcgacagca gtatttccga aacccatagc
    41821 ctggtcctgg aggatatcaa ccgggacggc ctgcctgacc tcgttaccgg taaacggttt
    41881 tttgcccacc agggcaaggg gcccgggggt cttgagcccg ccgttctggt ctggtatgaa
    41941 ttgctgaaag acggacgcaa ccggccggtt tggatcaaac accttatcga tgatacctca
    42001 ggagtaggca ttcagttcgt ggtggaagat gtaaataaag acggactgcc ggatattgtc
    42061 attggaaata aaaatggagt attctatttt gagcaagaaa aaaaataaat acgcggcctg
    42121 gccggcttat tctgaccgcc ggctgccgac tactgaaaaa tggtaacttt tgaagaatta
    42181 agggaaatac acgcgctgag tgagttgccg gatgagcacc tgcaatggct tttggaccac
    42241 tccgtttacc aggaatttga agacggcgcc ctgatcggga aatacgggga agcggccgaa
    42301 aacatgtggt ttttgctgga ggggaaggtt acgttttata tgaatgtaaa cggccggctg
    42361 gtgtattatt ttacttttga aaacaatgct acaaccggtg gagtgagtgg gctgatccct
    42421 tattccagga tgaaaaccta tcccggaaat tcgtatgcca ccggcaaggt caggtccatc
    42481 gccctgcata aaaaattctt tcacgaactc gaagacctca atcccgattt catccagaag
    42541 ctcatcggct atatgaccga aagggcaaag gctttcgcca ccacccaatt gcaacaggag
    42601 aaagtaagcg ccctgggcaa gcttgccgcc ggcatagccc atgaactgaa taatccggcc
    42661 gcagcgatca atggcattgc cagcgaactg accaaacgtt taaaccggaa ttaccagctc
    42721 accgaatccc ttcttgccgg gcaccttctg ccagaacaaa tcagggatat cggtgcaatg
    42781 gtccaaaaaa aggaaaacga tcgggccgtg acgcccaaac cttccctgat acagcgaatg
    42841 gaaatcgaag aggacctggc cggttggctt agccgtatga atatccccaa tcccatggaa
    42901 gcctctgaaa ctttttccga agcgggctat ataacggaag agctcgaagg cctttgcaga
    42961 caggtgggaa aagaagccgc ggtccatgcg cttcaatggc tcgaaaacct gttgagttct
    43021 caaaaaatca tcaaagacct gggcaatgct tcggcccgca tttccaacct ggttggggcg
    43081 atcaaaagcc atgttcatat ggaccgtacg ggcgatattc aatccaccca cctccaccag
    43141 gatattgaaa acaccctcac ccttttcgga tacaaactcc gcgaaaaaaa catcaccgtt
    43201 cgcaaaaaat tcagcgacca catggtcccc gtgcccgctt atgtgagcga gttgaatcag
    43261 gtatggacca atatcattga caatgccatt tatgcaatgg ataaaaacgg cgaactggtg
    43321 attgaaaccg cggtggaaga ccaccgggtc agggtgagca tcattgacaa tggccccgga
    43381 attccaaaag aaatccggtc gcgcattttt gacccctttt tcaccaccaa aaaagttggg
    43441 gaaggaaccg gcatcggcct ggacatggta aaccgtgcag tcaaacacca taacggagaa
    43501 atcagggtaa gttctgatcc gggcaggacg gaatttgcca tttacctccc ctttgaagaa
    43561 aagcagcaat gaattttaat ttattaagat aacccatgaa actgccttat atcctaattg
    43621 tcgatgacga ccttcaggta ttgagggcca tccagcagga tatccgcaat caataccgca
    43681 acgattataa agtggcagcc acgggatctg ccctggaagc cctggaactg atcaaagccc
    43741 tgaaattgcg taacgaaacg gtagcccttt ttatatccga ccagcgcatg ccggaaatgg
    43801 aggggattcc ctttctggaa aaggcccacg cacttttccc cttcgccaaa aaaatcctgc
    43861 tcaccgctta ttcagatatc gaggcggcca tccgcgccat caatctggtg aagctggatt
    43921 attaccttca aaaaccctgg catccgcccg aagaaaaact ttttcccatc gtaaccgatt
    43981 tgctgaacga atggcatgcc ctgtacaaac cccagcagga tgccctgcgc atcatcggtt
    44041 ttcagtggtc tccaaaatca caccgcctga aggaattttt atcgggcaac ctggttcctt
    44101 atctctggct ggatgtggaa cgtgatgcgg atgctgaaag atacctggtc agcgcacagg
    44161 tttcaaaatc ggccctgccc ctggccgttt taaaagacgg ggcctttctg accgaccctt
    44221 ccctggccga actggccggc cggataggcc tccagtcaaa ggcatccaaa gaaatgtacg
    44281 atgtgctcat catcggcgcc gggcctgcag gactggctgc gtcggtgtat gggtcctgcg
    44341 aagggctcag gaccgtgctg attgaaaaaa gcaacccggg cggccaggcc agcagcagtg
    44401 cccgtatcga aaattacctg ggtttcccta ccggcctttc cggggccgaa ctgacccagc
    44461 gggcgatcgc ccaaaccctg aggttcggca ctgaagtcct caccccgcag gaggtcaaaa
    44521 ccatcaccgt aaaagacggt tataaaatca ccgaaatgat ggatggttcc ctgatccaca
    44581 gcaaaaccat tgtgatcgca tccggggtag cctataaaaa actggatatt ccaggtatgg
    44641 accaattcag tggttcgggg gtttattacg gttcagcctc tgtagaagcc catgcctgcc
    44701 gcaatgaaat tatttacatc gtgggcgggg gaaattcagc ctgccaggcg gccatgtaca
    44761 tgagccaatt ctgcaaagaa gtaaacatcg tgatccgccg ggatgccctt tcccagacag
    44821 cagcccacta cctggtcgaa aacatcagga acgcccccaa tatccgcgtc atccccaata
    44881 cagacgtcat aggctgcgcg ggatcaaccg tgcttgaaaa cattaccttt aaaaacacgc
    44941 ttaccgggga agaaaaaacc ttgccggcca aagccctttt tgtttatatc ggcatgaaac
    45001 caggcaccga ctggctgaat gacctggtgc taaaaaatga aaaaggattt attatcaccg
    45061 gaaacaacct gctccaggaa aaaacatacc ataccttttg gaaactcccc cgcgaaccct
    45121 atatgccgga aaccagcatc cccggtattt ttgcttccgg agatgttcgg tttgaggcca
    45181 tgaccggtat ttcttccgcc gtgggagagg gcgctatggc tatccgtttt gtgcgaaaat
    45241 accttcagga gatataggcg tcccaaagaa gggcgcggag tttttcgcac ggagaaatga
    45301 ggcgcatgga gctcacgggt ctcacggagt tagctggagt tccgcaaccc gaatgcacgc
    45361 ggagatcacg aaggtcacgg agaaaataaa cattcttttt tcacatcaca ttaaaataaa
    45421 caactccgtg ctctccctcc ctccgtgtga acctcatcag ggtgaccggc gcctctggcg
    45481 cccggcaacc ggtatgcgca cggagatcac gaagctcacg gaggaaataa tcattttatt
    45541 ttccaccacc ataaaataaa caactccgtg ctctccctcc ctccgtgtga acctaatacg
    45601 gccggcgcct ccggcgcccg gcaaacccta tgcgcacgga gctcacggag cacacggagg
    45661 aaataatcat attattttcc accaccgccc acgaaataca aaaataatct ccgtgctctc
    45721 ccaccctccg tgtgaacata atacagccgg cgcctctgga gcccggcaaa cagtatgcgc
    45781 acggagcaca cgaagctcac agaggaaata atcattttat tttccgccac ctccttcgaa
    45841 ttacaaaaat catctccttg ctctccctcc ctccgtgtga acctcatcag ggtgaccggc
    45901 gcctctggcg cccggcaaac agtatgcgca cggagcacac gaagctcaca gaggaaataa
    45961 tcattttatt ttccgccacc tccttcgaat tacaaaaatc atctccttgc tctccctccc
    46021 tccgtgtgaa cctcatcagg gtgaccggcg cctctggcgc ccggcaaccg gtatgcgcac
    46081 ggagatcacg aagctcacgg aggaaataat cattttattt tccaccacca taaaataaac
    46141 aactccgtgc tctccctccc tccgtgtgaa cctaatacgg ccggcgcctt tggcgcccgg
    46201 caaacagtat gccacggaga tcacggagga aataaacatt cctttttccc atcaccttaa
    46261 aataatcttc tccgtgctct ccctccctcc gtgcgaaccc aaaaacaaaa aaactctgtg
    46321 ctctccaacc ctgattttat atttttaagc cctggcctca caggccttca attttaacct
    46381 cttttacaat gaccaataaa aaaatcaata ttgccatcgt cggcctggga ttcggcgccg
    46441 aatttatccc catttaccaa aggcatcctc atgccaacat gtacgccatt tgccagcgca
    46501 cccaatccaa actggatgag atcggcgatg ccttcgggat tgaaaaaaga tattccgatt
    46561 tcgcagaact gttaaaggac cccggagtgg atgccgtgca catcaacgcc cccatccatc
    46621 tccatgcaga acagtccctg gccgccctgc gcgccggtaa acacgtggcc tgcacggtac
    46681 ccatggccac cacggtggag gagtgcaggg ccattgtcga ggcggtgcag gaaaccggcc
    46741 ttacctatat gatgatggaa accgttttgt acagccggga atttcttttt gtcaaagaaa
    46801 tgtatgataa gggggaaatg gggaggatcc agtttctgcg cgcctcccac cagcaggaaa
    46861 tggctgcctg gccgggttac tgggaaggtt tgcctcctat gcattatgcc actcattgtg
    46921 ttgggcctgt gctcggactg accagaagtg aagcggaata tgtgtcctgc ctgggatccg
    46981 gacgcattga cgaacacctg atcccaaaat acaattctcc ctttgccata gaaacctgcc
    47041 atatcaaatt cagaaattcg gaccttgccg ccgaagtcac ccgttccctt ttcaatacgg
    47101 cccgccaata ccgggaaagt tttgatgcct atggttcgct gaaatccttt gaatggacgc
    47161 aaatcgaaca tgaagaccac gtcattcata ccggcgaaat ccccacccgg gtaaaagtgc
    47221 cggattatgc acatcttctc cctcctgaaa ttgcttcctt tacccaggca ggagtttacg
    47281 acgccgacag ccatcagcat ctttcgttta tccagggggc cggccatgga ggctcgcatc
    47341 cgcatctcgc acatcagttc ctgatggccc tggttgaaaa taaggatccc tatcccaatg
    47401 caaaacagtc cgccaacatc acctgtgtgg gtattttggc gcatgaatca gccatgagcg
    47461 gaggtcagat catacggctt ccggagttta cgctttcata attcacccgg gtgattatga
    47521 attattggaa ctggtaatat tgtaaaaccc ttaaagccct taaggtattc caccggctgg
    47581 gtttcccggc tttttccatt tcaaaatggg tctctccggg ataaggagac gccagtttcc
    47641 acagcccggc gtccgttcgt ttaccgagaa ttacttctat ggcttcggcc attctgggct
    47701 cgtatttaac ccgggcctgt tgaaaatagt ccagggcttt gaggatatcg taatgccatc
    47761 gtccgggaaa acacattctt aaaaaagccg gattgataat ttccccggtt ttatctgagc
    47821 ggaacaggcg gtgtattaaa ataaattcct ggctttcctc tgcggccttt tttaattcat
    47881 ccaaccggta accatacccg tttttttcat attcccaaat gccctccgca acagaaaggg
    47941 tactgtgcag ggagctgtgg accgcccctt tccggttgga ctggcaatta aagcctccgt
    48001 ccttcatttg ctggcccaga agaaagtcaa ccacggattt taaatgcttt tctttcacat
    48061 taaaataaca ggcatagttc agcaacatgc cattcacgca tacatcgcta ctctgcgtcg
    48121 atccaatggg gttgattccc ccatcagagg ctttctcctt atcaaaaatc agccgaagcg
    48181 tttcttttat tgctttattt gccggcgaaa tacccaggtt ttttaaatcc agcagggtat
    48241 aatgactgga tgtccatttg ggctgataaa aaccccttcc ccaatgcccg ttggaatgac
    48301 ggcggtttaa aaagccaaac ccccagcctt ctttttcgat tttatttctg agacccttgt
    48361 gttcatgacc cagcaggtcg cggtaaacct ggtacctgat ggaaacgtcg ccttccaaaa
    48421 gccaggacag gattttatcg tttcccgcag gagcattcat ttatattcgc ggtttttgac
    48481 ccatacacgc atagctgcct tttaattcaa gcctggtcct ttttcagggg attcttcacc
    48541 tccagggtgt ccttttttca caggcaagtc ttttttcgca tatttgatgc ctttaaaaat
    48601 aagcagatat ggcaagcttt aaaatcataa gccttaagag ttttctcttg cttctggttc
    48661 cttatttact gaacgcccaa acccttgacc tgccccgcag tacgcctgaa gccgaaggcg
    48721 tttcttccga aggcattggt atgtttctgg atgcctgggc aaaaagcaag catgaaccgc
    48781 atagttttat gatgcttcga cacggcaagg tgatcgcgga aggatggtgg tcgccttacc
    48841 gggccgacct gaaacatacg atgtattcct gcagtaaaag ctttgccgcg acggccattg
    48901 gttttgcggt gaccgaaaaa agactgagca tagacgatcc ggtcatttct ttttttcctg
    48961 aaagcctgcc ggacaccatc agcccctttc tcgcagaaat gaaaatccgg catttgctga
    49021 gcatgaccgt tggacagcaa ccagacccta ccggcgcagt ggtcacccaa cacgacgact
    49081 gggtaaaagc cttccttgca ctaccggttc tgtatcagcc gggaagccgg tttttataca
    49141 acagtctggc cacatatatg ctttctgcca ttgtacagaa agtcaccgga gaaaaagtag
    49201 tggactatct ccggcccagg cttttcgaac ccctgggcat caccggggca gactgggagt
    49261 ccgatcccca cggcatcaat accggtggtt ggggacttcg catcaaaact gaagacatgg
    49321 ccaaattcgg ccagttgttt ctgcaaaaag gacaatggaa tggaaaacaa gtccttcccg
    49381 catcctgggt ggaagaggca tcgacgatga aaatccttca gaacccttcg gcctcacagg
    49441 ccctcaggga ttccagcgac tggctgcagg gttatggata ccagatgtgg cgctcccggc
    49501 acaatgccta tcgcggggat ggcgctttcg gccagtatat aatcatcatg ccggaccagg
    49561 atgccgtgat cgccatcacc agtgagaccg gagacatgca ggatgaaatc aacctggtct
    49621 ggaatcatct gcttccggcc attaaaaagg ataaactccc cgcaaagccc agggcgtcca
    49681 atcttctgaa acaaaggctg gctgcccttt ccctgcccct ggcttccaaa acgcctgcgc
    49741 cggcatggag caaaaccatt tcgggcaaac attttttact ctcccccaat gatcagttcc
    49801 tgggcagcct gcggttggat tttcaagcgg aatccagcaa gcttggtttg ttttcccaaa
    49861 aagacagttt cattttttcc tttggatcgg gaaaatggat ctccggcgaa accaaaagac
    49921 tggggccgtc cctggtcgcc ggcgcaaagg gtcatttcgc gggccttcct ccttctaaaa
    49981 ttgccggaag ccatgcctgg aaaaacgaca gcaccctgga actcagcctg cgttatatcg
    50041 aaagcccgca ccacgaaacc tggacctttg tttttaacgg ggataaaatc agggtcgaaa
    50101 ccctcagcag caataaccga aaccggattc agtataaagg ggccattacc ccaaaaccca
    50161 cccggctgat tgtccggggc gatgatatgg gttactccca ttccggaaac ctggccctga
    50221 tccaatcctc caaagggata caaacttcga tcgaagtcat tgtgccctcg ccctggtttc
    50281 cggaggcggt gaaaatgctg gaacaaaatc cggggctgga tgtgggggtt cacctggcta
    50341 ttaccagcga gtgggacaat atcaaatggc gccccgtttc ggattgcccc agcctccggg
    50401 atcccgatgg atatttttac cccatggttt ttccaaacaa aaattatccg ggacggtcca
    50461 tccgggaaaa tgcctggaaa attgaagagg tggaaaaaga attccgggcc cagatcgaac
    50521 ttgccctaaa aaaaattcca cgcatcagtc atatttcagg acatatgggt tgttccaacc
    50581 tttcggatga agtgcgcgcc atgaccaagc ggatcgcgca ggaatacaat ctggatatcg
    50641 atcttcaggc cctgggggtt aaatacatgt cctattccgg ccccaaaaac agttcgaaag
    50701 aaaaaatcca gagttttatc aacgggctta aaaacctgga tcccggccaa acctatcttt
    50761 ttgtcgatca tccgggcctg gatgatgcgg aactcagggc catccatcat atcggatacg
    50821 aacaggtggc ggaagaccgg caaggggtca ccgacctttt taccaattcg gccgtacgtg
    50881 attttataga aaaaaacggg atccgtctga tcagctatgc cgatcttgca aaagataaat
    50941 aatcaactac cttccccatg cataccgatt acgataccat tgccaaagaa taccaggcct
    51001 ccaaactgct gccctggaga cagcacacgg aagcctatac tttttttaaa ctgattggaa
    51061 acctggaagg tcttcaagtc ctggaccttg cctgcggcga aggtttttac acccgtcagg
    51121 tcatgcttag gggtgcttcc cgggcagtcg gagtcgatat ctcggcgggc atgatccagc
    51181 tcgccagaga agcggaaacc agccgtccgt ttggtattca atatcatgtt tgtgatgtgc
    51241 tggaactcga tctcggccaa acttttgacc tggtctgcgc ttcttacctt cttaattatt
    51301 caaggacggc tgccgaaatc ggtcagatgt gcgcggtcat ttccaaacac ctgaaaccag
    51361 gcgggcgttt tgtcaccatc aacagcaatc cggatcaccg ttgcccgccc ggggccttgc
    51421 ggcattatgg ttttacccgt gagaatgaat cttatcagga aagtgctgaa gtgatctacc
    51481 gcttttacca ggaagacggt tcgcatatcg cggtcaccaa ttatcacctt gaaaaaaaaa
    51541 cccatgaagc cgcattttta aaagccgggt taaccgatgt ccgctggcat tccctggagc
    51601 tttcacccga aggcctgcag gaatttggat ccgattattg gaaggctatt gtggattgcc
    51661 aaccggtgat tggaatttcc tgcatgaaac cccggtaatc ttcccccgga cagggccttg
    51721 tgggaatttc cgaatacttt aaacattgca ttcaaagaag ggctttcaaa tggtcattcg
    51781 ttggtcagat ataggttagc cattttttcc cgggatttct ggctttatat ccggcatacc
    51841 ttttacacag gaaatacaaa atccccacat tgaccgcaaa aaccaggaat aataacggaa
    51901 ggctccagcg atattcctga gggacctgcg catagggggc ataggtgaaa aattcctgat
    51961 gcatgcttcc tgttttaatt ttatatataa acagggcgct gaggtggatc aggggaatgt
    52021 gcaataaata ataataaaaa ggcacccttc caaagacttc aagaactttt gaaattccgc
    52081 ctttcaattt ttcagccaca ggaagaaggg ccagcaaagg gccaagcgtc attaaaagga
    52141 ataagggcga agccgggtat ttttgctgat tcagcaaccg gaacagaaag ggagcagcgt
    52201 ccgtatcctc cgggctcctt ataatctgga tactgcccag gataataaaa agcaggataa
    52261 acagaagccc ggttcgaagc gccgctttac gcctttggat ctctccaagg gaaagaatat
    52321 acccaaatcc ataaccggca gccatcactc cgatccaggg caccagcgaa tagaggacgg
    52381 aaaccccgct gaacgtttca aatcctgagg gataaataaa ttcccatcct tttccaaaag
    52441 acgccctgat ggattcgggc aaaacttgcg gtacccggct aaaccaggat tgaaagacga
    52501 ttacagccgt tccgattaaa cccacaaccc ggggggggag ccataccagg gtggccagca
    52561 ataccataca ccatcccagc atccagatca cgccggcaag tataaattcc tgatagttaa
    52621 tatgaaaagc ccagaaaaaa cgaagtaggg taagttccag cacgaccagg atcaggcccc
    52681 ttgaaaggag aaagcggctc aggctcccgg catttccctt ttttaaacca tataaaaaag
    52741 cgctcgtgcc tgcaaaaaat gcaaaaccgg gcgcacaaaa atgggtgatc catcttgtaa
    52801 aaaatacacc cggatccgga cctcctgccg gaatcccgga gtacacccgg acatgatcga
    52861 tggccatcag caccatcacc aggccgcgaa tcacatcgat ggacctgatc cgggcagcat
    52921 ggccttctgg cctttccgct tgcatggata taaatttttg gggttatcgc gtttagttta
    52981 atggttttcc tttcaaaaac ctttccgtta aatctgattc ctttatacta ccaccggcct
    53041 aatattgtta aaaatacgaa aaccgctaaa caaaaatttc cggttttatt gaacgaatcc
    53101 ggatctttaa ctattcagtc caaatttttt tgtcccatgc ggctcattca attatttcac
    53161 aaacagggac aacggaggct tgccctggtt cagggagatg atctggtcct tatcctgaat
    53221 tataccaccg cttacgatct cgcctgtgcg gctatagatc agcaaaaaaa tccgatggac
    53281 tgtgttggac aaaacctttc ggatgaaaaa ctgccttatg aaggtattta cgatggacga
    53341 agcgagtgga aaatacttcc tgcctttgat catccgatca atccccggta ttgtatgttg
    53401 tccggaacag gcctgaccca caaagccagt gcggaaaacc gtcagaaaat gcatgaattg
    53461 caggaaaaag gttccctgac cgacagcatg atcatgtatc aatggggcct ggaggggggc
    53521 aagcccgcga atggacggtc tggggtccag cccgaatggt tttataaagg aaatggttct
    53581 cagttaagag ggcatctcga tcccctggtc atcccctcat acggcctgga cgggggagaa
    53641 gaacccgaaa tcgcgggaat ctatgtcaat gatgaatacg gcaatcccca ccgtatcgga
    53701 tttgccacgg ccaatgagtt ttccgaccat gccatggaaa agaaaaatta cctgtggctg
    53761 gccccgtcca aaatccggaa ttgcagcctg ggccccgagc tttccctttc ccaacatttt
    53821 aaatcactca gcgggaaggt ttccattgcc cggaaaggaa aaaccctatg ggaaaaaacc
    53881 atccataccg gcgaagacca tatcacccat agcctggaaa atctggaata ccatcatttt
    53941 aaatacgaca accaccggct tccctgggac gttcatattc attttttcgg tgcggatgcc
    54001 tttagttatg gagccgggat cgaactggag catggcgacc tgatgagcat ccaatgggaa
    54061 ggaatgggct gggctttggt gaatccgctg cactcaacgg ctctggttca ttagcaagaa
    54121 tccttataag gttttgtgta aaacacccgg actttagcgt ttaaacagat aattctcctt
    54181 ttccaatttt atgtcattca ataactgtat gcccgccgga ttgttcggat aggcattgag
    54241 gctcatttca atggcgcgct cggctttatc atattcacaa atgtttttat aaattaccgc
    54301 cagggcataa gataccgttt cattataagg cgtgtattcc agggcttcga ccaggtgatg
    54361 gattgcggcc ttataatcat ttcgtttcat ggcttcaaat cccaggtaat cctcctgatt
    54421 ttcccgtttc accacggcgc acaagggtac gcccccggct ttgacgatat ggatggttcc
    54481 tttcgggggc cagctcctat tcagcagctg gtttctgttt ataaatcttg aatagaacaa
    54541 ggcgtaatcc cagtattttt cgctgcggta ataataggcg gtgtacttaa gatccatatt
    54601 attggaggaa tcccccaaat aaacctgggc cgggtacatg ccgttggtga tcaccgtatg
    54661 gcgtttcttg ttcaactcca ccctttcctg tttctttagc cagctggaag cttcttttat
    54721 ggaaagcatc caataatccg tttcaaaatg gccataggcc ttttttattc ctccaaacaa
    54781 aggattaaaa taagcgtatt gatatggatg cagtgaaact aaagcataaa ggttttccga
    54841 aagcaagccc gccaacagca aaaaggacac gatcctgaaa attttagatc gaaatgaagt
    54901 aaaccataaa taccatccaa aggttgcgat cagcaccagg ggcggataaa tgaatataaa
    54961 atgcctccaa ccatcgtgca gggtagagtc cttgaatatg gcatagaaca ccggaaaaaa
    55021 ggcggcgaag gccaggacag acccttcaaa gaagggaata tcctgtaatc tttgtttgag
    55081 caaacggatg aaaaaaaata aaccgaaaat ggaaaacaat ggaatggtcc ggaggatcca
    55141 ttccggaaga tagttccagg gcagcctggc ggagtttata tgttccccgt taaataatac
    55201 gttcatggtg atgggctgcc tggtaaattc atacagtgcc tttaaaggat gggtgaaggg
    55261 gtgttggagc gcataaggcc aaaaaaggat tccggccagg taggccaaaa aacccacgac
    55321 cagggtatac ccaatgctta ttacaagctc tttgtttgaa aaagattctt ttttgattgc
    55381 ttttctcgtc aacaacaagg ctgtgaagaa gaataaatac acgatcaaca acaatcctcc
    55441 cgccctggta ttcagcgcaa tcatgacccc gaacacaatg cccgacatgg agcttatgcc
    55501 tggcctgggc aattcccgga taaaccgtac tataaaatat aaagccatga tatatcccgc
    55561 tgcaaaaggg acatcctttg gattgaccat gcagtctccg aaaaaccggg tagaaaacag
    55621 cataaagacc agggtcatga acgccagggg ccatcccgcg acctgccttg cgaataaggc
    55681 cgaaaacaga atggtgataa aaccgaagag cgcgcataat acccgccggg tatagtgaaa
    55741 ttccggagta tattcattgt ccaggcccaa aagcttattc aggctgatcg cggtcagatc
    55801 aaacaatcct ccatacagat gcatattccc ctccgggacg tataaagcag aagtgtcctc
    55861 cccgaaagtg gaataatagc tccatagctt ttttgaatag ctgacctgga atttttcatc
    55921 tccgtttacg ccataatcga agctcatcag gcacatgaac aggcatactg ccaacgataa
    55981 aatggtgaaa actttcgggt tgtataaatg ttgattttcg aaggatacgc ctgattgttc
    56041 cggcctttgt tttttatggt tacccggatc tggcgccgca ggggatccct gttttggcgc
    56101 ttcctttttc acaggcatgg ggcttgctgc attaggtgcc aaatgtatta tttaaaattg
    56161 actaaatggg atccgccagt acgtaacggc cggatagaat tctttcccta tatttccagc
    56221 ccgcttcctc ccgattcttc cgggggaggt cccaaaatga tcgcccggtt aaaaaagctt
    56281 ctttccaatc ccttctttta ccactattcc aggcaggtcc ttttattggg tatgcccctg
    56341 aaaacctggg ccgcttatgg caattactac aataaaaatg agcggattgc ggacctgggc
    56401 tgcggacctg cggatatcct gagatatacc gatccgccga ataaacccgc cttttatctg
    56461 ggaatcgacc aatccggccg gtatttaaaa cgggccgctt cgcgagccgc ccaaaagaat
    56521 atttcctcgg cttttataaa tttaaatctt gacctgctcc cttcaaacga agggataaaa
    56581 acccagttga tccgctccct ggacgagtat caaattacca ccgtcaatct gttcggggta
    56641 ttgcatcatt tgaatgatct ggccgtcgtc actaccctca acgccgtgtt tgattccgct
    56701 tccgtccgtt cattgaacac ccaggacgtc ctgttcctgg cccggaatcc aatcaataat
    56761 ttttacgctt ccttagaccg aggcgaatac gtaagaacag aagcacaata tgatgccctg
    56821 cttaataaat cgaagtggca aaaaatggat aaagcctggt ccagggccgg ggtccgtaag
    56881 gtaaaatata tacactaccg gttgaaaaaa taattacctg aatggtaaac ccggttaatt
    56941 acgcggctta tatcttttta attttttcat ggctttcaga tattgcaccg ccaggggcag
    57001 gatccttaca tggctctccg gagaatccgt ccactcgacc ggaagttcac atatcctgaa
    57061 gccctgccat tcggccacgg acaataattc ggtagaaaaa aaccaggttt tgctcctggc
    57121 tccgcccgca accaattttt cataaacctc tcttttcata aacttaaaac cgcacatgcc
    57181 gtctgaaaag tgaacgccta gatagctcct ggcaatcata ttaaatgccc gtgaactgaa
    57241 ttcccggata aatttcctgc cttttattac ggacccttta gccagcctgg aggcgtatac
    57301 caggtcaaag ccggatgtca aagcgcttat tgcttccggc aaatgtttca ggttcgtagc
    57361 caggtcgagg tccatatacc caatgatagg ggccttcgat tgactccagg atgattgcaa
    57421 ggccaggcca acacctcttt ctcccacgcg gatatatttt atgcgcgcaa attcttctgc
    57481 cagcttttct ccaacgcctt gggtattatc cgtcgatccg ttgtccgcaa tcacgatttt
    57541 ccaatccggg tacagggaat attctgttaa taaaaaatga tgcagaatga gcacctgggt
    57601 ttttaggttc ttttcttcat tcaggacggg gagggtaatt tcaaacatat tcagacctct
    57661 gtcgcagata tacaaattta ggcaaagacc tggtttgaat cgaccttacg tctgattttt
    57721 tactttttat tcgatccatt tcattgaagt ttaaacgttc aattaattat ctgcattagg
    57781 aaaatggcag actgagtgaa tgcttcgtat cggttcgcag ctgactgtcg atgagtctta
    57841 cgaaggtgga ggctccgtgg gccgagatcc agatcgaagc atcgggacaa agggcgaagc
    57901 aggtaacctc gatacccgat gtgtaactcc tgaaggaaat aaggtaaact caaagagcga
    57961 attgtatcag aattatccca atccctttga ccagggtacg gtgatcggcc tgcggctggc
    58021 acaggagggc tgggggacgc tgagcataca cgacgtgacc agcaaaacga tccgctccat
    58081 agaaagtgac tggacaaagg gctatcacga ggtttggcct gaccggaggg agatcggggc
    58141 gacgggagtc ttgtattacc ggtttgaatc gggattcttc acggacagca ggaagatggt
    58201 tttaatagag taggttgttt ttaaataagt cgccggctga tttcttttct ggaccctttc
    58261 gttatttatg atccatatac tatggccgta ttcattcaac tatttttact aagctgaagc
    58321 cggttttatt cctttttaaa aattacggta ttcataagtt ttgatacggg tctgtattgg
    58381 aagcctttta aatactttac cctctcggaa tccaacagtt gttctaccgt ttcgtatttt
    58441 aattcgatga gcaatacctc cggcttgatt tcgttccagt caatggcccg gaggatatct
    58501 ccttccagac cttcggtatc gatgctcata aaatcaatga cttccggcac ctggacgcct
    58561 tctaaggctt ttgataaagt agtcactgca atggtttctg tttttataag tttgtgattg
    58621 cctgcctggt attgggccgc ggcatcgact gaaaacgtat tcaatgccgg ttcctcgaaa
    58681 taatgatact ttaattgcgc cggcttgcgg tataaaccgg tgttgaaatt cagatcccgg
    58741 ggcctctctt ttaaaaaaaa tttaaagagt tcaggattgg gttcaaaatt tattcccgac
    58801 cacccctttc ggtaaaaata ataggtgttt gaaaacctcc gcggatgatg cgcgccgaca
    58861 tcaatataaa acccgatttc ctttccccct aaaagacggt ctgttaatat atcctctcct
    58921 tcctgggaat atatccgttt gcccctgctt tgtacaatat gcatttccag aggtttgagg
    58981 tacctcagat accatcgttc cggtataaaa gactttaact tcatggcatt cttttaccga
    59041 atattcgatg caggtgatat ttgatacggc tcgaaatcgg gcctttgtat ttatagatgt
    59101 tcttaaaggt atagtcgtcc gcctccttat ccactgaccg gtctttgggt tttatatagg
    59161 gcgggggcca ttcttccgct tttatgatca ggtggtgaag tgcaattatg gtattggtgc
    59221 ccatgccaaa gcctatgttt gttaccaggt tcttttgggg agtaatcgat attccgccgt
    59281 aataccagca ggcatataac cattggtaag accagtaatt atttaccgcg ggtttacgct
    59341 gatatttttc aaatttacca ctccaatatc ttttttcctc cggggaagta aattgtttta
    59401 tgtagtcctc cgattgttta aatgtttccc agtttttaag ctccaaacta taatgtgacc
    59461 aggctcgctt ccaggttgcc catccccaaa cgtgggcgta tttggaataa taataagacc
    59521 catcgccccg aagtattgcc cgctgaaaat tatttccgtt tatatgaaaa atggtgtttt
    59581 catttttgta ttgccccaac atttgggtac agaatgagaa aaaacttact gaaggcagac
    59641 aatcgtcttc caaaatgatc ccttcatcca cctgatcaaa aaaccaggtg atcgcgccgc
    59701 ttacggcgtt tttgcaaccc aggtgttggt tccggtacag cttatgaacc tcacaaggcc
    59761 agtctatccc ggatactatg tgtttcactt cttcacataa ttccttttca cccggaattt
    59821 ccgtcctggc cgcatcggcc gcaataaaca gtctcgaggg tttcactttt tgcaattggc
    59881 caaataccaa tcttgtggta gatggcctgt taaacaccaa aaaaagcacg ggagtatcgg
    59941 tcatctgtaa aaaatgaatc cgggttcaaa tttatcaagc attatccatc cccttatccg
    60001 gactatcttt ttgcgcgcgt tctcctttta acaggaatac gataatgggt ttccctttct
    60061 tcatgttccg tttttgttaa ggctcgcttt ttctatcttt atctgaaaag caactgcagg
    60121 aaccttccat ttaaggcctt cccatttcca caataaaaat gccatgggga ttttcaggtg
    60181 gctccataag gttgccttca agccaaaatg tttttgctgt atccgccatc tttccagtaa
    60241 agccgtgttt ctcctttggt cagatatccc gccacgctgg aaatcacaaa ccgttatacc
    60301 caaatttcgg gtgctttcag ccccctgggt aatccgtatt gcccagtcca ggtccgcgca
    60361 aacctggtat tgcgtatcgt aaaaaagggt tttggtcttc tttacgatca atgcctggtg
    60421 acaaatcacc atgccgttcc tgaaactgag ccagtttagt tctttgggga tttttttatg
    60481 cctgcggctt ttaaatcttc cttcctgatc cacaaaaaca gcatccccat ataatatatc
    60541 tttattttca tgatggttat aaatataaga gattacttgt gggtcatgaa atacatcccc
    60601 cgcgttcaga aaaacaacat agtccccttt tgccaatcgg attcctttat tcatggcaaa
    60661 atataacccc tcatcttttt cgcttatcca atctgtaatc acatcctgaa agtcctggat
    60721 taccgatgcc gtatggtcgc ttgaatcccc atcgacaacg attaactgta ctcttttaat
    60781 tttttgctca cggatattct gcagggtccg tttcaggttt ttggcgtcgt tgtagcacac
    60841 cgttatgatg cttaaccatg gcttgttgct gtcgttcatg cctttctgtg gttatcctgt
    60901 ctttcctgac ccggaaggat gccggtccgt attcttttgc ggcccgtcca ggtcgggtat
    60961 aggggtccat tgctttttaa gggcagttcc tatggatgtc aagacccata accctaaaaa
    61021 agtgccggca taaaaagaaa tgttgttggc ggccatctct gggggggcga atttaaattc
    61081 aatttgatgc tttccggccg ggaccggcat cgctctcaac aagaaattga ccctcacatg
    61141 gtttgctggc cggccatcaa tcctggcatg ccaccccttg tccgggccat accagacttc
    61201 cgaaaaaaca gcaaattgtt cactggaggt ttcagtttca tagataagat ggtccgggga
    61261 gtattgcaca agccggatga tcccgttttt ctgataggac ggcaggacct ctaaattttt
    61321 aaattccttt tgcagcagga ccgcctcttc tgctggatcc atactattta tagcttcaat
    61381 ctcttcgcgg ggcgtattca ctgtatttac cctggataca aaccaggcat tgcccagggc
    61441 atccgggttt tgctccaaaa cccctttttt atcaatgatg tatttggtgt tcatcatgtt
    61501 cagcgcattc ctgttgtttt gactgaagtg ccgttcgacc atatcctggt accggctcaa
    61561 tttagtcgca ctgtatccgc caatggtttt atgaaaatac gaagggatag ccgtgttgaa
    61621 agtatttacg ctgagatcca aaacacggta ggagagggaa gagtccctga gtatttcctg
    61681 atccaccgct ctgggcttga atattgaatg gattgacggt tcggttacaa aattaccggc
    61741 attgacgtac cttttggcga ccatccaaag atcaaagccg acgagcaggc caaaggccaa
    61801 tataaagtcc tgtggcttca gttttttcaa ggcatataag tataaaaggc ccgcaccaat
    61861 aaataaaata accagcgccc gtaccgtatc tgaatttaaa taactcctgc gttcctgcag
    61921 caaggcctca taaagttctc gattggtttt ccaatccgga tcgcccaaat cggatttcat
    61981 ttcaaagatt tggggcccta aaaccaagat caaaatcaag gctcccgcca aaggcaaaac
    62041 agtcttttta aataggctca gaatgctttt ttttgtccag gcatgctgca taaacaggta
    62101 caacccataa atggaaaaca agggcactag ggtggataaa acgttcaata tggaattcgg
    62161 cgccctgaat ttattataat acggcacgaa ttcaaacaat ggtttattca atatcgaaaa
    62221 atgatgaccc agcgattgca ggaccagaaa agccgcagcg gtcaagacca tccattttaa
    62281 cggacctttt accaggatga gccccataat aaaagccaac accaataaaa tacccagata
    62341 atccggccct ttggtattgg gtaattcccc ccaatacatc ggcagcgtaa aacgatcagg
    62401 aacggctatt ccgttttttt ctaatgcagc agccattttt gacttttttg accaaacttc
    62461 attggttgat ccgcctaccg cgcccggtac caataaggac atcaagtctt tatatccctc
    62521 actccactgc atggcatact cccaatccag cccgtttttg cctcctgctg aaccgtcgct
    62581 ttccttggcc gtatggccac tcaggaccga tcctcccctg gacgtttcgc cggcataaga
    62641 ccgcataccc atgagcagcg aataattgac cagaaaggaa gcgatcaccc cgaacaagag
    62701 acccagggcg gggcgtatca atgtgtttac tctttttttc tttaccctca gaatggtttc
    62761 tgccagcaca tatatggcaa gcaatatacc ggcctggtag gtcatttgga tatgattcat
    62821 cctgagactc cccgccagtc caaatgcaaa aaccacagat ccccatttcc agtttttatt
    62881 gaacaataaa taggctcctg ccagaatcat gggcacaaat cccagcgcgc caattttggt
    62941 caaatgaccg gagcttacca ggacaacatg gttcgttgca aaggcaatgg aataagcccc
    63001 gatcaatgaa agccagggcc ccattcccag gcagcacaat cctaaaaagg cgcccaatga
    63061 caaaatcaga taatatttta atgacccgga aaaagaaagg ctcaggcctt tgatcagaaa
    63121 ctcaaagagg ttgcttttgg tctggggaac ataaatttga taggtgggca tgcctccaaa
    63181 cattgcattg gtccaatacg ttggacgacc ggtcttttca aaatgcgcca tcgcttcgcg
    63241 ggccatgccc ctattattga tcatatcgct ttggaacaat actttgccac ttagctcagg
    63301 ggaaaaaaag accaggttga ttccagccag gatcgaagct gccataaggt atggacctag
    63361 gaatttaacc agatggggca taatgttaaa gctttgctgc tgcttcgttt aactcaaggc
    63421 gctttcaagc cgaagatact acttaaatag agacctgaat ccggccgatc ccggttgaat
    63481 cgaaggcctg ttttgttaat ttttaaaaag ccctgctcct gcttaaatcc attattaatt
    63541 ttgtccccaa tagaggattg tccttatttt attttctttt tccatgaagc tttcaaaatt
    63601 cggttattta tccaaacacc ggatggccct tctttatggg atggccctgg ccttcctttt
    63661 gtttttgctc aagtggctgc aatggagatt tttgatcgtg gatcactcta ttgaaattta
    63721 tattggcctc attgcctttc tctttaccct cctcggcatc tggatcgccc gcaagctgac
    63781 caaaccccgg ttgaaaaccg tcatggttga aaaaacggta cttattcccg cagatcccgg
    63841 atttaaactc aatgaaaacg aattatccag gttgaatctg agcaagaggg aactggaggt
    63901 gctccaactg atggcccagg gcctgagcaa cgaagaaatt gcctcccgct tatttgtctc
    63961 cctgagcacg gtcaaaacac acggttcgag gcttttcgag aaaatggagg tccggcgccg
    64021 gacccaggct gttgaaaagg ccaaaaggct gcaaatcata ccctgatctt atcctccttt
    64081 ggtatgaata tgggcgaaag agccaaaaat cagacttaag tatgaatggg agggcgaccc
    64141 aggggcctat ttttaggcct tcaataaatt ttttctatga aaaaaaacat tctgattttc
    64201 ggattgatca tgggcgcggt actttccgcc aacatggtct atacggtaaa cctgtgctac
    64261 cacagcggtg attttaaggg caacgacatc cttggctatg cggccatgat cgtgatcttt
    64321 tcgttcattt tcattgccgt gcgcaattac cgcaacaaat tcaacggagg cctgatcggt
    64381 ttcggttccg cttttaaaat gggagcgctc atcgctttta ttgcagccac ctgttatgtg
    64441 ctgacctggt tgttttatta ttatcttttt gttcccgatt ttctggacaa atacatcccc
    64501 tatgtcctgc aaaacgccgc ggaaaagggg gcgagtgcgt ccgaagtggc aaaacagacc
    64561 gcggacatgg ataagtttaa ggaaatgtat aaaaatcccc tgtttgtcat attggtcacc
    64621 tatgccgaag tgctcccggt ggggctgatt gtggccctcc tcagcgcttt gatcctgaag
    64681 aaaaaaacaa aggaccctga aatggcggtc ccttaaatcc ggtttctgcc aggcggcgga
    64741 gtggtttatc tgatgtattt gatatattta tttcaaaaac catgaagcgg atcgaatcca
    64801 tgctggataa cctgttcagg cccctggaat tcatcggcca tcgctttatc ctggctcctt
    64861 tccgggcctt actgaaaaaa attgatcccc tggccgaggc gattgaaggc acctggctta
    64921 aaaaatccct ccaggtcact attttcccgc tctttatgat ccttacggtt tgggtggggt
    64981 tcaaattggt agaaggcggt tataccataa ggactttcgg gggcaccatc gtcatgttcc
    65041 tgattatggg tttcattttt gcccccctgg aacggctatt gccctttagt aaaaaatggc
    65101 aaaacgatga ggatatgccg gtagatgtct tactgttttt tgggaataag gtatggggag
    65161 attatatcaa taaaccgctt caactggcga cgatttccct ggtggtccag aaaatatccc
    65221 cggaaatcgg tcgtgacctt tggccgggat ttctccatcc agccttgcag gtatttgttt
    65281 tattatcggt caatgatttt ttccggtact ggtaccaccg ctggatgcat gaaaatgaat
    65341 ttatgtggcg gtggcatgcc gtacaccatt cttccgaaag actgtattgg ttcaacggca
    65401 cccgttcgca cccgctggaa ggcctggttt ccaattttct atgggcgatc ccgctggctt
    65461 ttgtaaaagc tcccgtggag atcgtttttg tcaccggcct gctgggtcgc accattggcc
    65521 gtttccagca taccaatatg gatgtgatcc tgggcccttt tgattacatc ttttcttcac
    65581 ccaaaaatca tcgttaccat cattccaaaa agatcgaaga gggaaattcc aattatggcg
    65641 gagacgtgat cttctgggac ctgctttttg gcacctttca tatgcccagg gggcaacaac
    65701 ctggggatga tatcgggatc gatcaaatgc ccaattatcc gaaaacgttt attggcctca
    65761 tgctggctcc ctttacctat ggccggataa aaaaagaagc aaagaaaatt atggagaaag
    65821 gaggatcgtg aaaagcggat ggcgtaagtg atgcccctaa atgaaactgt gtataatcag
    65881 tatactttta atccatgaat ctccaacgta ttggttccca aggtaatatt ttcacccgtg
    65941 gataagggga ttcatcaaaa actaaattta agggaccctg aaccaattag aacagtgttt
    66001 tttcattcaa tggactgaac catcggatta aacatttgtt aactttccac gtatgcattc
    66061 cggaagtcat tactcgttta aggaagtttt actgtggacc cggcgggata tttatctcca
    66121 tctcgtcatt gctcttttcc ccactgtgct ctatgttctt tttgattgga aatggctggc
    66181 cctgccctgg cttcccattg cattgcttgg aaccgcggtc gcttttgtgg tgggttttaa
    66241 aaacaatgca tcttatgacc ggctatggga aggccgtagg atatggggat ccattgtcaa
    66301 ttcaagcagg agctgggcta tccttgttaa agattatgtg accaaccggc acgtcctcaa
    66361 taaacttacc gtcgggggtc tgtccgctgt ccacaaaacg ctttatgaac gacattttgc
    66421 ctggcttacc gcattgcgat ttcaactccg tgagcgaagg ccctgggaat ccatttataa
    66481 aatccataac ctggaatata aaaacaaatg gttccgggtt gaagaacacg aaacccggct
    66541 tgccgatgcc cttcggccct atctctcccc tgaggaatat gaggtcgtga tgtcaaaaac
    66601 caataaagcg gtccagatca ttgccctcca gtcttctcat ttgaaagaat tgctggaaca
    66661 agggcttatt gaagatttcc gtcacatgga gcttgaaaaa atactcgttg aactgtatgc
    66721 acaccaagga gcctccgaac gacttaaaaa ctttccctat ccccgccagt atgccacttt
    66781 aaacctttgg tttatccgcc tttttatttt cctgattccc tttggcatgt tccaggaatt
    66841 tgaaaaactg ggagaccacc tggtttggct cagtgttccc ttcagcgccc tatcgggttg
    66901 gatattcact actatggaaa aaataggcga atcttctgaa agcccgtttg aaggaagtgc
    66961 caacgacgtg ccgataacag ccatgagcag gaatattgaa attgatatga gagaaatttt
    67021 taggggaaac ccatcaactc aaacctattc caattcagaa taatatcctg gtttagtctg
    67081 gcaccttttt aaacttctga tttaaattcc taattgccat gaaaatcacc attctcggaa
    67141 cgggaaacgt cggctccgcc cttggaaaag ggtggattgt tgccggtcat tccgttctgt
    67201 ttggagcaag aaactccgct tctcctaaag ctcaaaaaat ccaggactta cttccagatg
    67261 tccggatcca atccgtagaa gatgcggtaa aaggtgcaga ggtgctcgtg atcactaccc
    67321 cgcctgaagt cgtgcttgac cttattccct ctctcggaga cctttccgga aaaattatta
    67381 tcgatgccac caattcggtg aggaccaggc ccgggcctta tcccactgct tttcatgcaa
    67441 tcaaagccct ggctccttcc gccgaagtgg tcaagtgttt taatacgacc ggatttgaaa
    67501 acctggcaga tccggtctat caagggaccg gaatcgatat gtttgctgcc ggaaacagcc
    67561 tcgtggccaa acaagccgcc agtaagcttg cgcgagacct gggttttgaa acctgttatg
    67621 attttggggg cgacgataag gttgaattgc tggaaaaatt ggccctgagc tggatcaatt
    67681 tggccctgat gcagggactt ggaaggaata tagcatttaa actggtaaaa aggtaagcgg
    67741 agcggaccct ccctggaaag gagctccagg gaccagctca tgttgatcct ccgattcata
    67801 gcgcgatttt cctattccct gtttatcttc acaggaatta atcctttcct atgaagtcga
    67861 tttatttcct cctgttttgt attcttattt taatgccacg cccctccggc ctccatgcac
    67921 aggccgcgga cggcaaactc cggatcatca tgttcggcgg ccatccggat gattgcgacc
    67981 agaaaggggg gggtactgct gccctttggg ccaaaatggg ccatgccgta aaatttgttt
    68041 ccgtcaccaa cggggatgcc ggccatcaga atatgaaggc ccctgaactg gctaaacggc
    68101 ggtatgaaga aacccaggaa tgcgcccgtc ggctgggtgt gagttatgat gtgctggaca
    68161 accacgatgg tttattggtg cccagcctgg acgtaagact tcagatcata aaaaaaatac
    68221 gcgagtggaa tgccgatgtt gtgcttgccc cccgcccaaa cgattatcac ccggaccacc
    68281 gttataccgg ggtgcttgtg caagatgccg cttatatggt ggccgtaccc ctgatcatgc
    68341 cagaagctcc cgcccttaaa aagaatccgg tttttcttta tttccaggac cattttcaaa
    68401 gacccaatcc attccggcct gatatttcag tcgatatcac ttcggtcttt gatcagaaga
    68461 tacaatgcat cgatgcccat gaatcccaga tgtatgaatg gctgccctgg attggccatt
    68521 acgccgacca ggtgcctgca gataaggcgg atcgcgtcaa atggctgacc aaaaggttga
    68581 gccggccgat cgacgcaaaa atcaaggcca ctctggtcaa atggtatggt tcatcgaggg
    68641 cgggccaggt caggcacgcc gaatcctttg aaatctgcga atacgggagc cagcccaatg
    68701 ctgaagagat taaacggctg tttcccatgc ttggcaaata aacctttttg catgaaggtc
    68761 aggtttttta taccggttat tctttctttg tatgcgcttg gggtaagcgg acaagcgtct
    68821 gaaatcccgg tctggatggt gaaaaaaacc ccggatttta ctttttcagg caacggggcc
    68881 tcacccgaat ggaccaagac ggattggctc gaattaagcc gtcggaaaaa cgaccaggta
    68941 gcctaccaga ccgcggtaaa actgctgtat tccgaaaccg gtatgtattg cctgttccgg
    69001 tgcgaagacc aaaccattac cgccaccatg aaggaagatt tcctggacct ttggaatgaa
    69061 gacgtggtgg aagccttttt ctggccagac cgctcatttc cggtctattt cgaatatgag
    69121 ttgtcgcccc tgaattatga actgcccatt cttgtaccca accataaagg caatttttta
    69181 ggctggaggc cctggcatta cgagggtgac cggaaaaccc ggcatgccac ccatgttcat
    69241 aagaacggcg gggatatcca atcctggacc gctgaattct ttatcccctt tgttttatta
    69301 aagccgcttc ccaacagcat tcctcaaaag ggtagccgtt ggcgggccaa tttttaccgg
    69361 gtggactatg accagggaag cacgacctgg tcctgggcgc aggtgcgtac caattttcat
    69421 gattacgaac gctttggcat cctggtcttt gaataaatga taagccgcct ggaagttttc
    69481 ccttaattct taatttcttt aattcatttt ccatgcgctc cgtgtttttc ctgctttttt
    69541 tcattcatct gggttttctg tttgcacagc cggcggctta cgacacgatt attctccggc
    69601 attttaagga acaaaacctg gccagtctca gtgtgggcct cattgagaat ggtaagatca
    69661 gttataccaa aagtctgggt atggccaacc tggaaaccct gagtcctgcc acagacacga
    69721 ccgtctataa gatcggctcg ctcagcaaac agtttatcgc cgcagctgtg gtcaaattgg
    69781 cttgggaagg gaaattgtct cttaccgatc cggtaaataa atttttcccg gatgcccccg
    69841 ggcactggaa agccattacg gtcacccatt tgctcaatca tacttccggc ttgcgaaggg
    69901 aatccccggc cttcgacgcc atggaaaaca aagcggacct tgacctgatt cgcgcctcgt
    69961 atggggatac cctggtattt gaaaccggga cccgctggca atattgcaat ctcggatatt
    70021 ttatgcttgc ggagatcgtc cgtcaggtat ccggccaggc tttttccacc tatatggagc
    70081 aacaaatatt ccaaagtgcg ggattgctga agacgaggac cactaccgtt tcagaacttg
    70141 ttccggcacg tgcagacgga tactggctcg gcgtcacgaa acaatggctg aactcgccaa
    70201 attatatcgc cctccgtcca agcggcgctt ttttatcaac gatccctgat cttcttaaat
    70261 gggaactggc cattcaacaa aatcaatttc tttcaaagga ggcatggtcg atgttgtggc
    70321 ggccaggcac gcctaccggg aataaacaaa ccgacggaac cggtatagat tacgggttgg
    70381 gctggaatgt cagccgtttt aaaaaccatc tttgggttta ccatggcggc tccctgcccg
    70441 gattccgggc catttattac cgctttccgg aagaaaacaa cgccatattg atactttcca
    70501 ataccgatca cgccaatttg ggccggctgg caaacgacct ggctgaattg tggtactgat
    70561 tatccttctg ttttgaaaag gttttatttt tattcattgc tctgtttgat tttttcgcct
    70621 tcccggatca gatcattggc aaaccggaca atgcggtctc ccctggataa accgccgaat
    70681 acttcaattt gatctttcca actaaatccc aggctaaccg gaataaacca ggtggtgtct
    70741 gatcttattc caaccaggta ttggctctcc tgggtcgtaa caagggcatc gggcggaata
    70801 agaaaggtgc tgtctttgcg tcccagtttc atttttgcct gaacatacat tccgcttttt
    70861 aattgacctt ccgggttggc caaatcaaac tgccagattt cactttttgt attttccgtt
    70921 aatgcattgc ttcggcgggc aaacaccgcc ctaaataccc ggccggggat ggagggaaca
    70981 gtaaaagaaa ggtttttatc cttcaataca gccgaggaat aggtttccgg aacgccaatt
    71041 tccactctca tcaggccgtt agcctccagt tctaatatat gttgcttccc tccgctgccg
    71101 gttaattccc cttcatcgga atagcgggct gtaacaacgc ctgaaaacgg actgcggatt
    71161 tgtaaaaaat taatggattc ttttaaaatg gataaggaaa aagtttccgc ctcccaattc
    71221 aggctgtcgg caatcatttt attgcgggcc tgaatgacct cagatattgg aatggtgccc
    71281 ggtgttcttg ccagatcagc tgccctttca gattcgtcct tactggtgag atattgggca
    71341 taggcggcat gtttgcgttc attccgttct gcccattgca taagcaattc cggtgcgtct
    71401 atctctgcca ggacctggcc cttcgtaacc ggggtaccca ggtcaacata tattctttta
    71461 atataaccat caattcggga atagattttt attttttcgt ttgcccttaa acttccggga
    71521 agggtgacct ctttggatat catcgttggc aaaagctcca aggtgttata aatacgttca
    71581 tcggtgtgtt ctattaatga tcgccctttt gggggggtct tgcaggaaaa aatggaaaac
    71641 aagacaatga atgaacaaga tatttttgac ctatagttat tgttcatcgc caggaagtaa
    71701 agatattgaa tgattcccca cgccggtcat gaatgacccg tagattgatg gcagaatcca
    71761 taaagttata atagtggaca gcgtcagccc cccaatcacg gccaggccca ggggtacaat
    71821 ttgttttccg gtttccccca tgccaacgga caaggggatc atgcctgcaa tcatggctac
    71881 ggcagtcata aggatagggc gaaggcgcgt tccggctgct tcggaaatac tgttttcagg
    71941 ggaaaggccc atttttcgaa atccctctgc cgcattaatc atcaatattc catttgcgaa
    72001 agatactccc agcgccatca cggttcctgt aaacgactgg atgtttagcg tttgattcgc
    72061 caatgttaat aacagtaaag cccccacaat ggaggcgctg gctgaaagca agatcacaaa
    72121 accaatacgc aaactctgaa aatttgcggc cattaataga aaaatggcca ccagggccac
    72181 cagcaaacca tttttgagtt cggataaagt ctgatccaag acaagagatt gtcctttctt
    72241 aaggatcgtg gtacccggct gaagttcaag ggtgtttaaa atatcatcaa tctccttcat
    72301 ggccgaacca aaatcctttt cttccagatt ggctgttaca ttgaccattc tctgttgatt
    72361 gtatcgctga tagacgccgt aactgctagc gggaaagata cgggctacgt ccctgaggag
    72421 tatacgcatg ccttccttcc caaggggtat gttttcaagg ctttcgattg aattcatctg
    72481 attttgagga aactccgcct ggatctggta ggtattccct gattcctgat ccagccaata
    72541 taccggggag acgaatcggc tgctggatgt ggcggtcacg atagcattat taatatcctc
    72601 gatagtcaag cccattaatc cgacccgctc ccggtcaata tcaatcttca aggaaggata
    72661 atccagtgga atcccgattt gcaggtcgcg taaataggtt agggttgtaa gttttaccat
    72721 taatgattcg gctattgcct tactttgctc catatttcgg cccaggacag caatgtctat
    72781 gggtgttgtc gagccaaggc tcatcacccg ttccaccaaa tctgcgggtt cgaaagatat
    72841 gctgacgtcc tgcatgtttt tagagaaatc atcccttatt ttttctctta aatccgccaa
    72901 tgaaaccccg ctggcagagt ttagttttac ctgcaccacg gcctcatatg gcccgtttgt
    72961 ccataaatag atggtgttgt tggggaaact tgatggctgc gccccgacaa aagctgaagt
    73021 gatttccaca ttttctcttc ccgcgagcgt ttcaatgcgc atcagcgcct tcctggtcgc
    73081 ctcttccgtg cgctccatac gggttccggg ttttagtctg agcctgaatt gaaattgacc
    73141 cccgtctgcc cgtggaaaaa tttcagtacc aattgaagca aacaaaccca ccaacgatag
    73201 ggcaaggatt agaaaatagg taatggttat agggcgtttc catgccatcc aacctgcaat
    73261 gcgttcacga tatttttgct gcatccggaa ggcttttcca ttatggtgtt gcgataaacg
    73321 gttgttgagc aaccaggctg aaatcaccgg tacaaaggtt tgtgataata aaaaggatat
    73381 gatcattgca aatccaacgg ccatggcaag cggctggaac atagcctttg gcagtccgct
    73441 cataaaaaaa gagggcacaa acaccatcag aatacacagt agaatcagga gctttggaaa
    73501 agaaatttcc agcgaagcct ccaggatggc ccggcgtttg ggttttcctg attcctgatg
    73561 ccggtgaata ttttcaaccg tgaccgtagc ttcgtcaacc aggatgccca cagccaaagc
    73621 caggcccccc agggtcataa ggttgagggt ttggccggtt atcgacaggc cggtcaccgc
    73681 cccgagcaaa gccaggggta tggtcagtac aacgatgaat gcgctgcgca aatctttcag
    73741 gaaaaacaat accatcagac ccgtaagcag ggccccaagc agggcttccg tcaataacga
    73801 tttgagtgat ttgatcaggt actcggtctg gtcaaactca taagtcaccc ggatgtcgtc
    73861 aggcaccgcc gcctgcatgg ccggtagcgc tttctttacg ttttgcacga cctgccatga
    73921 agaggcatcg cttctttttg ataccggtat atagactgac cgccttccat tgatcagcgc
    73981 gtaacccgtg gtcaggtcgg aaccgttttg cacggtggct acgtcccgga taaaagccat
    74041 aaaacgatct ccctttttaa caaccaggtt ttccaggtct ttgatgtttc cggccactga
    74101 attgataggt gtgatcaagt tttcatcacc aatcctgata ttgcccgcag gcgtcaccat
    74161 gttgcctgca gctatcgcat ccacgacctc gtcaggagaa atacccaggc ttgtcatctt
    74221 ttccgggttg acctttacaa ttatcgcttt ttgattcccg ccgaaagggg gcggagcgct
    74281 cactccttca agggaagaaa acatgggtct taccttaaac agggccaaat cctggatttc
    74341 agtcagggaa tggttttcac tgctgaatac caattgtccc accggcacgc tgcccgcatc
    74401 gtatctcacg atgaaagggg gcaccgtccc ttccggcatc tttgatttgg cacggtttac
    74461 gttattcacc acttccgcca tcgcctggtt catattggta ccagggtgaa actgaagttt
    74521 gatcagggcg taattttgca ccgattttga ttccacataa gccaggccgg ttaggtaaat
    74581 acaataatac tccaggttgg aggtgacaaa accttccatt tgttccggtg acaaacctcc
    74641 ataaggctga gaaatatata tggtcggcag tcccaggtcc gggaacatat cgaggggaac
    74701 tttttttaaa gaaagaaccg atgaaaggat catcgccaac accaaaacca gcacggtcac
    74761 tggttttttt agtgccgcca caatcagatt catacctgtt taagttgatt ggaataattg
    74821 gactaaggta atctggctgc ttttaaaggg acgattcagt ttctaagaaa agccttagaa
    74881 ggactgctgc ctgattgttt ttatccttgt ttcccttcct ttcttattcc ctccacataa
    74941 aaatttctgt caatagaaat acctgaactt ttaacccttc tggacttatt cgaacttttt
    75001 gggcgaaggg ttttcacggt ttcattatct agtgccgcgc caactattat tgcctaaaaa
    75061 cagctaatgg ttttcggttg ctg
//
LOCUS       DAOQXT010000007        74684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_344_length_74684_cov_7.082903, whole genome shotgun sequence.
ACCESSION   DAOQXT010000007 DAOQXT010000000
VERSION     DAOQXT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 74684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74684
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_74684_cov_7.082903"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..509)
                     /locus_tag="PKM27_01770"
     CDS             complement(42..509)
                     /locus_tag="PKM27_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06010.1"
                     /translation="MSRKNPWLLTSIACKSGSAARGIQRTLKKPNLSKTATLDELKVA
                     VRSTNRPFKIVWVSRLRGGSGQIQTNLTFGRKALMLANAPGKTLVLVKHKITSGPNEM
                     ALETHFLFIFLLQASTGKNLCINCPADPPFRKAFCSFRGSKTSITKPDFKAAL"
     gene            718..1017
                     /locus_tag="PKM27_01775"
     CDS             718..1017
                     /locus_tag="PKM27_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR06011.1"
                     /translation="MDQTHFMSANMRFFEIPVAGGLQLSSACPEMEGSFHNMKHVIYF
                     SEVHKITEQLKWIFNHPLEADKVRRAGNKMVMGKHTYIQRFDLILEHLGLKQDKT"
     gene            1021..2526
                     /locus_tag="PKM27_01780"
     CDS             1021..2526
                     /locus_tag="PKM27_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06012.1"
                     /translation="MGKSEVSLTEAKGFIGHSAQPQILLNDVLKFPVSLLLLLLLGIY
                     WITGNEVPVIFSLAVLLFLYMISGLGHKLIIPEAMAFYIIMIYLLFPLWGYQRYPAQH
                     PLSTLFDVYMRVPREEYFEFAIPAVILFISAMFNAQRNNDISFTSSLFTSARTRLAGV
                     QQVGVVLIVTALMAYFLGPRMPSGLQYVLQLGFLAIIPGLFYLYFQPGRGLGKTVMVL
                     TGLAWLFYLAIKSTMFTLIVYMGIASLGFLFIGRKISMAGKVAVFSILLIVGLVMQYT
                     KANYRNLIRVNKAESGDIRVFSRLFLQNLVNIDQAVLDGAFFPIYMRGNQGYLLSRVM
                     EHVPGRKPHDGGRLLGKTLVSSFIPRLFWPNKPEAGGRFNIEYYTSLKLSRRTSMNVG
                     PIGEGYGAFGKWGGMLYMIFFGTALGLANRVFLNTCTKLPLLLFWQPLVFYEVIYCME
                     NDSMQAFNSLIKIAFLLFILNKIFPKLFKGKPLDSYAGYGDGGFPKLQRLD"
     gene            2727..4913
                     /locus_tag="PKM27_01785"
     CDS             2727..4913
                     /locus_tag="PKM27_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR06013.1"
                     /translation="MDGLSVYPRGFNQGKKSYSTVHLKRGKYFISETLILRGKIGFRI
                     IGEDPQHTILEWRGKEKDTMLWADGSAYFKVSNLTFHGGFRTGMEGIGIHWATRASGG
                     VNHFASLNIEINNCRFTGGMDVGVGGGSYASGDWTGANDSEVTIRYCEFDGCASAGIR
                     IAGFNALDYWIWNCRFMNCVIGVDCRHGNYHVYNSYFRQSALADVHNTGGYYTSLRGC
                     FSENSMAFSVDGGSSSNPFKRVFENNIVKQPKELPIQYHHIGKLSLYGNRIDRSQADT
                     LNRKNPVRSPDPQMDPSPVSVTYGSWYPSIFTILSLRNKFFYAAPFRFSSAHPSMLYQ
                     ADDKVGHTVSSEGLESFEKTGIQAVPVLKETVFRVPLNSTTDELQAIIQRASASGIEN
                     PVVQFEYGRYALNKSVFIPAHSRIKLRGEGLIYSSMLVIENPEIFKGEALFIVRGPTN
                     VSIQDLHIVGNTTRGGSKLIGIDFRNVDTRNARVVMDQLYCPAETSVFVDRYNYTLFE
                     KNNSFFSDGNIIIGGDEQKAGRGTLRFHAFGGQYAGTQVFNGARAVFKDCWYEGSRKI
                     PLSLVGDGEILIDGAMCAPNKLDSTTTIAIEGFRGKVTLANMYMYGSILLKPSMDSKI
                     FLWNINMNQVARPSLFAQETYPGQVAIAGITTDCNFPGELDCGGFKSYEDRFHNISDK
                     NAFILSNMEPGMNVMPVEGLLPGDLNQISVSRVSMEGMGIGLKFSK"
     gene            5170..6075
                     /locus_tag="PKM27_01790"
     CDS             5170..6075
                     /locus_tag="PKM27_01790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR06014.1"
                     /translation="MSHLVHLISEYDPDVVYLNSLYSVKFTFYPILAHRWLKHRSRII
                     VAPRGMLKESALRLKFIKKFLFIKAVRGMQLQKGIEFHGTNVKEGEEIRKALGGVRVH
                     IIGNLAKEIKSEWVQIGKSRGQLNIITVSRVHPVKNLHYFLTILRQLAQPVKFSIIGN
                     VEDMEYFRKCQEILSQLPVHIHVDFLGDIPHHEISPRLESNHLFVLPTLGENFGHAIL
                     EALSSGRPVLISDQTPWRNLEEAKAGWDLPLSDPGQWLKKLEEAASWDQEEFDAWCRG
                     AQEYARVHTNTTELIEKYKKMFSES"
     gene            6162..7889
                     /locus_tag="PKM27_01795"
     CDS             6162..7889
                     /locus_tag="PKM27_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNR06015.1"
                     /translation="MTILGLNAYHADSSAALFIDGRLQAAAEEERFTRIKHWAGFPME
                     SIRFCLRDKGQQLKDLDFITIGRNPKAKLWKKILLLVRQPALLLNLSDRVKNQSVIFD
                     LKQALAGVDGSLDLLSIKKRLMLIEHHRSHLASAFYPSPFEQAAVLSLDGSGDFTTTM
                     RAIGKDYKLQVLDSVDFPVSCGLFYTAFTQFLGFPNYGDEYKVMGLASYGQPRYADQV
                     KKLLRFLPNGLFDWDQAYFVHPTQAGFRYENHVPRVGDLYSGKFVKLFGPPRQKEEEL
                     SAYHKDLAASVQRVTEELIFHIASDLRKKTNLDNLCLAGGVAQNSVANGKLTDATGFR
                     QVYIPSAGHDAGISMGSAMYAWHHILGHKRTEPVRHAYNGAHFSNADIEQLLKSKSVE
                     FRKYEDEELYDIVTDKLIEPGVVGWFTGRAEFGPRALGGRSILADPRNPHAKELLNLK
                     IKRRESFRPFAPSILKEYTSEYFTRNGEVPFMEKVYPIREDKRALIPAVTHVDGSGRL
                     QTVDKNTAPRYYKLIERFRQKTGLPILLNTSFNENEPIVNTPGEALDCFLRTRMDMLV
                     MENCVVTRS"
     gene            8708..9448
                     /locus_tag="PKM27_01800"
     CDS             8708..9448
                     /locus_tag="PKM27_01800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR06016.1"
                     /translation="MTISIITPSLNNETVIRDCLESVRTQDYPEIEHLLLDGGSMDRT
                     LEIARSFPHLADILSGADEGIYDAINKGINRAAGEVIAVLNADDFYLQPGVISRVMKS
                     FREGKVDSVYGDLIYVSRKDPSKIIRTWKSGPFQKELFLQGWMPPHPAFFLRKDCYLK
                     YGLYRKELSISADYELMLRMLYLHSLPCDYLPETLVAMRTGGASNRSFKARWLANRQD
                     RAAWTMNGLKPEWLTLWKKPLSKLKQYL"
     gene            9942..10499
                     /locus_tag="PKM27_01805"
     CDS             9942..10499
                     /locus_tag="PKM27_01805"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNR06017.1"
                     /translation="MQHPDLKEFRLVGGTALALQFGHRKSMDIDLFGDAEVSGDQILE
                     LLNQYGFVVPVTRSKNINIFLLDGIKIDFVKYRYPWIEGCIVEDVIRMASPADIGAMK
                     INAITGRGSRKDFIDLYYLLKKYTLLEILGFFQTKYEDGSMFLALKSLCYFKDADLQQ
                     PPFLFENISWHDMKQSILNEYKKIV"
     gene            10503..11615
                     /gene="gmd"
                     /locus_tag="PKM27_01810"
     CDS             10503..11615
                     /gene="gmd"
                     /locus_tag="PKM27_01810"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR06018.1"
                     /translation="MKKALITGVTGQDGAYLSHFLLRKGYEVHGIKRRASLFNTARID
                     DIYQDPHVDNQRFVLHHGDLSDSTNIIRIVQEVQPDEIYNLGAMSHVHVSFEEPEYSA
                     DVDGIGTLRVLEAIRILGLEKKTRIYQASTSELYGLVQEVPQTEKTPFYPRSPYGVAK
                     LYAYWITVNYREAYNIFACNGILFNHESPLRGETFVTRKITRAAAKIALGLQDTLYLG
                     NLDAQRDWGHAKDYVELMWKILQYHQAEDWVIATGVTTRVRDFVRMAFAELGIELEFQ
                     GEGKEEKGVVIRSNLPEFQLPAGKSVVRVDPGYYRPTEVELLIGDSTKARTLLDWEPR
                     YTVQQLCKEMVQADVALFRRDQLLKESGLKIRNEFE"
     gene            11915..12292
                     /locus_tag="PKM27_01815"
     CDS             11915..12292
                     /locus_tag="PKM27_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNR06019.1"
                     /translation="MTENELATIVVDLCYQIHTQLGAGLFESVYEEVLYYELIQMGFK
                     VERQKGIPVRWKDKKMDIGFRADLIVDDKLIIEIKSIETVLPVHKRQMLTYLKLTGMK
                     LGLLVNFNEALIKDGITRIVNNL"
     gene            12574..13500
                     /locus_tag="PKM27_01820"
     CDS             12574..13500
                     /locus_tag="PKM27_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNR06020.1"
                     /translation="MKPDSKIYIAGHRGMVGSAILRKLKSLGYNNLVYAPSSELNLLD
                     QAAVDDFYRREKPEYVFMAAARVGGIAANNTYRAQFLYENLMMQNHVIHGAWQHGVNK
                     LLFLGSSCIYPKLAPQPLKEEYLLTGPLEPTNEPYAVAKIAGIKMCDAYRAQYGCRFI
                     SAMPTNLYGPNDNYDLKTSHVLPALLRKFIEAVKSGAPEVVLWGTGTPYREFMHVDDC
                     ADGCVFLMLHYDQEGHINIGTGLDLQIKDLAALIAGITGYKGKIVHDSSKPDGTPRKL
                     MDVSRINQMGWTAKIKLEDGIKMVFEEIKDQF"
     gene            13537..14580
                     /locus_tag="PKM27_01825"
     CDS             13537..14580
                     /locus_tag="PKM27_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNR06021.1"
                     /translation="MYHAIIMAGGAGTRFWPLSTESKPKQFLDILGTGKTFIQMTVAR
                     FEGWIPPQNIYIVTNEKYRGLINEQLPDLPDDQIICEPSRNNTAPCILLAALKIRKRD
                     PEATCLFVPSDHLIRDTDAFRENALEALEYASRHEAIVTIGIIPTRPETGYGYIKMEE
                     NQDGSVKKVEQFVEKPNLDSAKFYLSSGKYLWNAGMFAWHVNTIIREYHSLAPGIYKI
                     LSHGESHYNTGAEEAFLASQYPLTENVSVDFAIMEKSAHVYTVPAGFDWSDLGIWSSV
                     YEASDKDKQGNVCIGGKLLQEEAANNLVTLPQGKQAIIRGLEDFIVIDSGEALLIYPR
                     KNEQEIKKSVAAV"
     gene            14611..15726
                     /locus_tag="PKM27_01830"
     CDS             14611..15726
                     /locus_tag="PKM27_01830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR06022.1"
                     /translation="MHILFIPSWFETPQHPVKGKAMKDLALALAREGIKVNVLFQTGE
                     KLPPVSLAGPGVEIWYSFFPGRGQLYPIWNAGSLRSYLGTFKKYLTLHGRPDLVHIHS
                     YPVLAIAHLLKKKYGIPFLYTEHSSKIAEKKINILEQFLIKRYLGKQAPVFAVSRYLK
                     ESYAEYIHNDIHVMPNTIDFKVFKPGRTAFPRHLMMVNTLDQNKQVGLGIKAFTKWQK
                     GYPDACLHIIGDGPERHNLEQLIHQLGMKGKILLWGEKKVEDWLPLLQSASCFLLMSR
                     SESFGVAALEALACRVPVVALQNRGIDEFPHVPGLQILPLFSNELDISQRIDQAIREF
                     DKGQMGLTRAELLARFDFPVVAGKYLEIYKQFLKPLA"
     gene            15732..17009
                     /locus_tag="PKM27_01835"
     CDS             15732..17009
                     /locus_tag="PKM27_01835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR06023.1"
                     /translation="MTKVKILFISYYYHQQTLTATRNYYLSKALADAGYEVHVMGRMA
                     SDLVLPDKIKMHPVSAWDYRRILMFFGVRNGVTSSVVRPGRWIRWGYELLLTHPFNKW
                     LAEGGGFYLNKARRTGLEILGRHGITHLFSSYRPMADHFIAQDLKHKHPGLVWIGDFR
                     DVLWMEKENPHYQEKWIRSLIRDMDHLTAVTRGVAEFWASVYCKKAEVIYNGLPGKAV
                     QAVPRQSPSEKFIINYTGMIYTLLQGAGVFFDVLRELCATEDRFSESLSIQYSGLHAN
                     YWRTWMKQHGLLDWASIRHQLPVNEALEKQTNAQINLVLTWATLDVKGIIHGKFNEYL
                     MARKPILCLIEGDRDTELEGIYRKLPNSLLAYNREEDKNLIRQFILRHYRQWRQNGRV
                     EEIPQEVLDSYDWSKQIKGIEAYLSKSFNSLIL"
     gene            17024..18322
                     /locus_tag="PKM27_01840"
     CDS             17024..18322
                     /locus_tag="PKM27_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR06024.1"
                     /translation="MTVLIVTFTYPPAMSVNGFRPRYFAKAMAEQGWKVRVLTRHFSG
                     KETSAEEYRKPLSTAFSISEEGAVHVYRTPFKNSWFTYYEKGFLRRTGLWKAVYLIQL
                     LAGRTCQESYNAYFRKYLAQVLETNKPDIILVESGPTNLVRLVSKTAVKYGMPYVIDF
                     RDLYYHEMYRDFGLLPFNKKVKILLEKWYMRRSITSAQLVFSLNKEWLDILHIPTDKR
                     LMISNGFDSEIWNEIPPQKEFEDFTISIIGTLYPREFIHAFLGSLNVFLQNRPEKVKV
                     KFIAPGSKALVNLIRETLPFEQVEVVESRLPYREAIVIMAKSQILMYHGWPGYKCYIS
                     TKVYDYLRSGAKILIVPSDENGLDRLIEETGSGASCSTAAEGAAQLEKWYCEWLEGKL
                     IRRGIDTGQQDIFSRNSQSLILVKSLRNYVESHRIRLGRD"
     gene            18388..19260
                     /locus_tag="PKM27_01845"
     CDS             18388..19260
                     /locus_tag="PKM27_01845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNR06025.1"
                     /translation="MATIARQSIYTYRFRILGYGLNILLRPFKKTLPSAWIVIHNYDH
                     NLSICVNPVEHIGSTIFYTGFYEAKEIWALKRLIKPKEVILDIGANIGAWTLILAKLV
                     PEGRVYAFEPSCFYKQLNWNIQLNREIKNIVAQNIALGAYPAQATISNPNTEHRSSII
                     NYGNLNLKLDPDQSLMGDRIRVITLDDWVRQEKISRLDWIKLDVEGMELMVLLGGLDS
                     LIKFHPNLLVEINENTLGNYGSTALQLSSFLMQTGYRFFYRVGKKGVLKKYDSMSTSM
                     GESFNLICSFRALD"
     gene            19322..20455
                     /locus_tag="PKM27_01850"
     CDS             19322..20455
                     /locus_tag="PKM27_01850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR06026.1"
                     /translation="MFISDLIASIQVSQPQHYHTVGLHAHKIKWISPHHLWRDLHAYF
                     KDETPLQPRGSTFIELFELSKTIGLNPITRLNFIHTLIRKHIHLVGGMKCRPDLIHVH
                     VSFPSLPVGYELSKQFGIPFILTEHSSRFPDPRFESYGYDRETLNKYFLRSSANIAVS
                     ADLAKKFGFYDIQDMQVIPNLVSWAPPEIRIPGPPFSFILISHYLDPRKRIDLLIDAV
                     HLLRSSGKEFIVRMVGEGPMRNLYIEKVKAAGIADVIQWYGHVSDGHKQELLSASHCL
                     VVCSDYETFGVTVIEANAFGIPVLATRCGGVEEIISSVNGRLVDKGNSIRLAEGMEWM
                     MRNYSYFDRKRIQQDCYLRFSATRIAEKYLNVYDEILLKNKLS"
     gene            complement(20447..25405)
                     /locus_tag="PKM27_01855"
     CDS             complement(20447..25405)
                     /locus_tag="PKM27_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNR06027.1"
                     /translation="MNASETYRLLGLICIFQCLPGWLKAQMVQAGDTLYGPEWIRYDQ
                     TYFKFYISKDSLYQIRYNDLIQAGVPAGQINGAQFQLWRMGREVPLYVSNTGAWSEGD
                     FLQFWGIKNRSDMDAFLFSNPARDMLNPEYSLYTDSMAYFLTWVAPGNATRRVTLRPS
                     NTMLSAPDYVWVERLYEFHRLPFDQRYDFENTVSYSTYDACEGFGNGESRNYIQVIPA
                     ASPYTGNINGKLRIRLATNARQHNLRISINDAVQVIDSFWGYQVKNYELDIPNETLSG
                     NINLKIEGLDSPLDYFVLSNIRLVYPKQPGKPLAVGEVLKSAGSPYKTLVPPGLSQPV
                     LIDPDGAIWTQAIREDNQDYIELPQSADSGQVYILEAAAASPVGNLAKIELAPLLSDP
                     NSDYILIHHKSLLTSTQEYASYRQSSAGGGFRVRTVDVDEIYDQFAYGIPRHVMGLRN
                     FGNFIARKWAAPKYLLLVGRAINYRDLRVNNNINLYGHLHLIPTFGYPGSDNLIFASH
                     NSSVPVLPFGRIAATKNEEVITYLNKVKEYEGKLKNPSANEDLYWRKRVLHMAGGSPQ
                     ERFDAYLESMKEKIEGNAFHAQVTTVTKTSSDPVQGGLSEIVKNAINNGVALKVYLGH
                     GATSATEIGLDDPALFDNEGKYPLSFSLGCLTGNMHTTGISLSESFVLSPKGTIGYIA
                     SSGFGYPYALSNYGNEFYNLLGNKQYVGSIGNIHRESLRKFDRLRDFPTYSLNQQLTF
                     HGDPAVQLNYYESPDFTFDYSSFRFEPANVQSDQDSIRFKVDIWNIGKYSDQPVGIKV
                     EHGVPGGGTLEYRFSVVLKGTFETVSFSLPMPPNAVGINTLRCFLDPDNDFQELPAPF
                     AENNNPLRDNTGHSEISFAIYSNEARPIYPRNFGIISDADLVLKAYSSNAFSPESDYY
                     FEMDTARLFSSSLKRSATVHQRGGMIQWRPGLALSPNTVYYWRVAADTVGTNRPLIWN
                     ESSFIFLPGQGPGWNQSHDYQFNQNPQSQLIFFDTLHHRWENSPEQISIVASSINQSL
                     DPAEYSKVLFNGIRHTRNNRNFLSEFILTIWDPKIGMIRNPVGGRDGALNVFDVPAPG
                     YYFPMEKNTIEERSNLIRFLETGIEDGQYVIVINHIEPGASYYPEFWAHDSTILGKNL
                     FQAFESHGATLIRSLATELISYPYVFAFRKGHEVIDEKVSTDGLQVRSSFSLPLSRSF
                     GTFESQLVGPSIHWQKFEWSVNDERMDTENTMVVLGKNNQAFDTLIKTSASLVDLTGI
                     DPNSYPYLKFEWQASDTSKSGSLNMEYWRVYYTGYADLAMNSNEDYEFYKDTVDRGED
                     ARIRFSLENVGDYPTDSIRVTFSLTDPQNITTRDTLSTGPLGAGQKRSISNSFSTDQR
                     SKLQSLVIDARSKNGLQETTLLNNTGSISFYVADDLVPPTVSVLFDGRQILNNEIVSR
                     QPFIQIDLSDNKLFSPEDTAQIALSLKYPGSDQFIRIPSREYTLSVSGSKASVTYRPG
                     FVQTGSYTLRVQARDRSRNEAGRVPYEIDFKVITENTVSAVLPYPNPFSTQCRFAYTL
                     TGEKPAVFKIQVLTVNGRVVRELTEFDLGPLREGTHLTEGAWDGHDEYGNKLANGTYL
                     YRVVMKDNQGKSYSSFEDFEDSGGQDARRFFTKGIGKLVILR"
     gene            25441..26526
                     /locus_tag="PKM27_01860"
     CDS             25441..26526
                     /locus_tag="PKM27_01860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR06028.1"
                     /translation="MVFADINCLLFIGACLAQVFFLGYFIRPLRKNTVPVSKSSPPLS
                     ILIVGRNERSNLEKLLPLLVNQKYPDFDIHLVDDASEDGTADMVRDLIPRFPSIRLQV
                     LPRHCGKKKALETGLMQLDRELVLLTDADCLPATDQWISLMVESLDPGYDVVLGYSPY
                     EQREGPLNQVIRLETAYTAALYFGFARRNLAYMGVGRNLLYRRTALVHAFNLNAQRGL
                     KSGDDDLTIQELSGQGKINFRYDPPSWVYSLPEKNWKAWLNQKKRHVGTARHYRLRHQ
                     ALLLMWYGSLAGTWVFGLVAAFHFPLMLLVWGGYKGVSFKWIVRSMLELFGVSLSPSK
                     WLGAEGLYVGVLLYLLPFSTFKKWDKW"
     gene            26586..27485
                     /locus_tag="PKM27_01865"
     CDS             26586..27485
                     /locus_tag="PKM27_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNR06029.1"
                     /translation="MSINAQYSISDLSHYTGIKPHTIRIWEKRYNLLAPKRSCTNIRG
                     YDGCQLRKLLNINTLLHSGWKISHISQLSEEEMDAEVRMVMDRSEGSSKIDFLINGLM
                     VHMLNFDEQRFSQVFTQAVEQYGLKNSIVEIVYPFLKKIGVLWQIDDISPAQEHFASA
                     ILRRKVLSEIDRMPQADHSKNRIVLCLPPDEFHELPLLLAHYILLEKGINTVYLGSSV
                     PPEMAAETAKQTKSGFLFTLFMSGAPAEKIKGYLETIHENAPDLKIYFSGSPETCKKS
                     GLNDRIFSLEGIEAFERFAASLQ"
     gene            complement(27512..28600)
                     /locus_tag="PKM27_01870"
     CDS             complement(27512..28600)
                     /locus_tag="PKM27_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06030.1"
                     /translation="MEHQRDGGVSLRFRSWADSAWTETRTVASGQDWFVNWADYPALS
                     VFPGSEYNLLAHFLQKRPGGKTYDYDVKLALSVDQGAHFKVFEGLHDTVPAEHGFVTL
                     LPFSFDKILVTWLDGSQMNPGVDEPGHEGHMGHEGAMNLRAALVSVSGEISHNEVIDD
                     RVCECCQTDAAMTGRGPVIIYRNRNEQEERDIYSVRYVSGQWTTPQPVFEDHWKIGGC
                     PVNGPTICSRGNQLAIAWYTEAGGRPAVKAIFSNNAGETYGQPVEIPSANTSGRVDIA
                     WIDAGRVAVSQLEKTDEINQGDALIKLTILSPDGQVLSDHRLAETSSIRKSGFPVIES
                     KDSTLYLAYTQVLDSVNTRVIVKEIILP"
     gene            28843..30003
                     /locus_tag="PKM27_01875"
     CDS             28843..30003
                     /locus_tag="PKM27_01875"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HNR06031.1"
                     /translation="MAAILQEAKNYVKDLLTSQLNGTYWYHNWEHTEYVFEEAMALGE
                     SAGLSESEKEILGLSALFHDTGFVKAYQDHVSNSKVIAKEYLLSRGYGQEQTAGVLEN
                     IEATRMPHTGTGKLGALLQDADLALLASEDAIQKSENLRRELAVFENKHFSDAEWVKH
                     NLDFYKAVVYQTAEGKRKYEAGKEKIRDILKEKNKKSKKDESSLINSNRTAEMLFKTA
                     LRNHINLTQIADNKSNIMLSINALIVTFALPLMYEQMQSQQWYFLIPVFILGGTCLIS
                     IIYAALATRPGTVKGKFNLADIQAGKGSLFFFGNFFKTPLKDYIPAMKQALAKAENLD
                     DSAIIDLYFLGKSVGLKFELLRKCYSFFLVGITLSSVLFGIVFIIQAIGNQG"
     gene            complement(30085..31284)
                     /locus_tag="PKM27_01880"
     CDS             complement(30085..31284)
                     /locus_tag="PKM27_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNR06032.1"
                     /translation="MKFLNKTIYLLLCLTVMYTSKAQDSIPPKAFKVSGYADMYYNFN
                     FNNAGVGANLWGPAAGSRAFDINSNEFSLGLIQGKLSYTHDKVELVADLLAGPNTSLA
                     TLASFQGVSFPVSLGAGVFGIKQLYGTWKATDKLSFTIGQFGTHIGYEVIESSINFHY
                     SLSNLFNNGPFFHTGLKANYTISDKIGIMAGLVNTWDNFDDNNEFKSPVFQVSLFPMD
                     GLSIYLNYLSGKGDRAGSLNPVFDELPDANGFNTSIYDLTAGYNTGKFTFGVNAAYGI
                     YKAKENSVKTQFKKITKEEKDNPDWGGVAGYASVALSEMFSLGARFEQFSDYYSVRYL
                     GAQNSSFTLTSVISLSGGDLLLKPELRIDSSKGKNSINGHTDLYLDADGTTDSQATLG
                     MSAIFKF"
     gene            31641..33038
                     /locus_tag="PKM27_01885"
     CDS             31641..33038
                     /locus_tag="PKM27_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06033.1"
                     /translation="MNKSSRRRFLRQFGTGSLALSGGWFSQAVAEEYYERQVLPYNKI
                     YSANDRIGIGVIGCGIQGNFDLRAALQVPGAQLAGACDLYTGRLTRMKEVYRQDLYTT
                     RDYRELLQRKEVDAVIVATSDHWHSRIAIDALKAGKHVYCEKPMVHLVNQGHAVINAQ
                     KESGKVLQVGSQGISGIDFAKARELYRSGTIGKLNCIEASNDRQNAIGAWQYSIPSDQ
                     SPETVDWDKYVEVLSKKPAYDAKKFFWWRNYREFGTGMAGDLYVHLITGIHYVTGSLG
                     PSRILSSGELSYWKDGRNVPDVLLTILDYDETPQHPAFQVMLRCNFVSGAGGSGGTKF
                     IGSEGVLTKTGNGVKVQHSLMSKAPGIGGYDSLFTYPQALQDKMMAEYNAKWSEEDKK
                     RPTRPNIEYSAPEGANAHVDHFSNFFESIRNNKPNIEDATFGFRAAGPCLAANESYFQ
                     KKIIKWDPTAMKLKK"
     gene            33052..33594
                     /locus_tag="PKM27_01890"
     CDS             33052..33594
                     /locus_tag="PKM27_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06034.1"
                     /translation="MIMPPGRKALPAVLLGFGLCFASCRKEDDAFLFRMEYDQIIALP
                     PGLNTVETYTFVIRDLPTHFQALLSTHGVAEESVRSVNPGAIRLSDELNQLDFNRFEK
                     VSLLASTGDFKNEKEIGYQETIPLNSSNQLQLFPTLVDARDIFSQNTFNLRLKIKLRN
                     FLSSTSNMRLRISMNARESQ"
     gene            complement(33686..34108)
                     /locus_tag="PKM27_01895"
     CDS             complement(33686..34108)
                     /locus_tag="PKM27_01895"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 family protein"
                     /protein_id="HNR06035.1"
                     /translation="MNSKVIVAALLGALTSFILGFICYGLLLMDYMSTNMIPGAMKTE
                     AEMGASSYVFIFLGSFASSLLFAYVFDRWANIRTARGGASAGALLGLLMILSFDFGMY
                     ATTNLLKMNFMLLDIALNVVFAAAAGAVIGWWLGRKPA"
     gene            complement(34286..34714)
                     /locus_tag="PKM27_01900"
     CDS             complement(34286..34714)
                     /locus_tag="PKM27_01900"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNR06036.1"
                     /translation="MNSKVLLAALIGAVASFIAGFIVFGLLLGNYMEAGMSPGLMKTA
                     EEMTGASYVYIFLASFVSSLLIAYIFDSWANIRTLQGGLRAGALIGVLMALSVDLGLL
                     ATTHMFSGLGKVIVDVLASTLVTAAGAAAIGWWLGRNPAG"
     gene            34881..35837
                     /gene="metF"
                     /locus_tag="PKM27_01905"
     CDS             34881..35837
                     /gene="metF"
                     /locus_tag="PKM27_01905"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNR06037.1"
                     /translation="MKVTEYFEKAQGQTLISFEILPPLKGGSIDSIFKILDPLMEFRP
                     PFVDVTYHREDFIYRKNDDGFFEKIAIRKRPGTVVIAQAIQNRYGVDAVPHLICGGFT
                     RQETEDALIDMAYAKVNNVLALRGDARKFEGRFIPEPEGHHYAIDLIRQIMDMNQGKY
                     LDADIVDGAKSNFCIGIAGYPEKHFEAPNMESDIHYIREKIKAGGEYIVTQMFFDNKK
                     YFEFVDLCRQNGITVPIVPGLKPLTRKNQIQSLPASFYINLPEDLVKAAEGAKNTEAV
                     KQVGIEWAVAQCQELKKAKVPSLHFYTMGDSETTRKIAEKVF"
     gene            complement(35856..37625)
                     /locus_tag="PKM27_01910"
     CDS             complement(35856..37625)
                     /locus_tag="PKM27_01910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNR06038.1"
                     /translation="MVNVILILLTAFVNPPAPAYIDPLVLEQFHKHPTADVLVLFKSG
                     TDGWLPLAGKMETKEDKGRFIFEKLRAEARVSQRSVRRHLTRNQINHRAFFIINGIHI
                     PAADYPLIEYLSGLEEVARIEFNSPYALVKPVQTVSDIRIPESRSPDLAAEWGIERIQ
                     ADTLWSMGITGKGTVLGSEDTGVELHPAIRGSYRGTLDFNTLDHHYNWHDAIHSLHPL
                     NKDSTDNPMNNPCGLDVQTPCDDHNHGTHTVGTMAATDFQIGVAPEAKWIGCRCMERG
                     WGTLTTYIECFEWFLAPTDLNGQNPDPSKAPHVINNSWGCPKEEGCNPSNFQTMEQVI
                     RTLKAAGIFVTVSTGNEGSLGCSSIQNPPAIYEPSFSVGATAFNDTIASFSSRGPVLT
                     DSSFRIKPNITAPGVAVRSTIRNGAYAAYNGTSMASPHVSGTVALLISAFPSLAGKVE
                     LLEDILEKSAEQKTLRFTCGADSGMVIPNIVYGYGRLNAFRAWKTAKEMLSTGIVNST
                     VVNIRAFPNPFSRGLVFDFAGGTLSGKLLIHDLHGRSMFTQSFISVDRIELVTSGWAS
                     GMYFYKIEAGRRLYTGKVVLVGK"
     gene            37799..39298
                     /locus_tag="PKM27_01915"
     CDS             37799..39298
                     /locus_tag="PKM27_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNR06039.1"
                     /translation="MDIAHYTLSDTERQSFEKLQEELIPQFQLQFPDPMAPKTVIIVP
                     SLSLDSEILSKISGHIHYEERLLCLLLLLRMPRTQVIYLSSMPIDEVIVDYYLHLIQG
                     ITHMHARKRLSLLACYDASNIPLTEKILERPRLMERIRSLVPPGHVAHIAAFNVTPLE
                     EQLALKLGFPLYGCPSSLSYWGSKSGSRELFRNAGIPMPPGYENLKSTEDVLQALMNL
                     KAEQPHLTKAVLKLNEGFSGDGNAIYTFGKLPKITEDLLRNNLKMVASDMVFDQFMEK
                     FNQMGGIVEAFLPGVQASPSVQCLINPLQEIDVVSTHDQILGGESGQVYLGARFPADD
                     AYRREIGELGYKLSKHMSTLGVLGRFGVDFVSVKDGEEWKHYAIEINLRKGGTSHPYL
                     MLQLLTGGRYDYKEGKYIMPNGQTRSYIATDALVYDNFKKLTPPDLIDVAICNNLHFD
                     GTTEEGVGFHLIGAMSQHGKLGVVSIGTTAERAEALYNRTISVLLGESR"
     gene            39340..41454
                     /locus_tag="PKM27_01920"
     CDS             39340..41454
                     /locus_tag="PKM27_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNR06040.1"
                     /translation="MFFKKALILFQVFGISLQAQSPLPQIKKWLDDSSYLETRIEDMK
                     SKTYRVFVSSGKAVPYAEPAASVSPELPDSLRMYSWGQSKEGKFVLKRGTDLFLYDGA
                     LRTLTADAEEEKVPQFSPDGKKLAYVKGGNLWVADLQNNLHRQLTSDGNESVYNGYAS
                     WVYFEEILGRSSRYRAFWWSPDSRMLAFLHTDDTPVPKFPVYRSEGNRGHLEMEHYPK
                     SGDPNPRVEMRLAELQTGDIQRVEEDSMADQYTAWPFWTPDSKKLIFQELNRDQTHLR
                     FYAYDPAARTKTFLFDEKYPTWVEFHEQIWFLKDNSFLLIHPLDGWWNIYKYQFPEKK
                     LSQLTRFDWRVNDIVRVDETQNRILFTGTGENKMNNHLYRINLDGSGLRQLTHGDGSH
                     RTDLSPGGSFFIDSWSNLSTPRSMYIANTEGKKIRELGIANDGNKIAGIKTEYFTVPS
                     GDGYDLPVVWNLPPDFESGKAAGKVFPVVISIYGGPDAGTLRNAYRDLSNDKLIQQGV
                     ILLSTDHRASGKFGKKGLDFMHRNLGKWEIHDYAKVAEWLKQQPFIDSSHIAIEGGSY
                     GGYITALALTKAPHAFTHGISFAPVIDWALYDNVYTERYMDTPKDNPEGYKQGSVLNY
                     TDQLKGKLFIVHGSADDNVHMQNTFQLIEKLQLTGKQFEMMIYPNVRHGWGGPRASHL
                     NNLELDWWSKQNFVAKKPEPKS"
     gene            41482..43404
                     /locus_tag="PKM27_01925"
     CDS             41482..43404
                     /locus_tag="PKM27_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR06041.1"
                     /translation="MKSSFNRPTVFPKTWLYFLIGLWASLSHCGQIPELPYEDRSHAS
                     RVFGKEKQYRLYLPKGYDRSTVRYPVVYFFHGWGGRHFTDPSAKLEYEKLGELVNKYQ
                     AILVMWDGNIDEKEPRPYNIGNHENITSNIQLSEYFPELVDYIDSTYRTLADRDHRGI
                     IGFSMGGFMSWFLAGKYPHLVTAAVNMTGSPEFFVGYPENHTLYPLRYTFKNLKGVRL
                     RFHNSTADELCKLNLEVHQGALWEGGLDYEYWTFEGGHKVDDPGKTEVFEKAVSFVAT
                     AFNNPLSKPDQWGHYDLYPQFSVWDYQVKSDKNEPGFIFLRRVSGKGFGLYTHRWLPD
                     GPPVNACRIEVKTAPVYKPGTAYIISRYSKADKKVSQDTVTSDPEGCLTIRADQNGYE
                     FGVALQEVSPDLVVGGYQMENQFRYLRTDRANKLTLTLFSRGPEITGNHKARIEVRSK
                     DTSVKVRMENPVIDLFSGERVFQSPVFTLECKKQAPPNAQPSQVKINLILHLDSMKFE
                     DELILPVLFEAPEATDLLIDDGRRWNDSLPILGIGNGDGLAAPGEEIMIYVNGHRTRF
                     YTDDSFVLQDKERLVDEVVPAKWPDGFTLNSVIRMDPSCPDGHQVEGLASYETKGFMP
                     IDRKLTWHRVKFRVKK"
     gene            complement(43433..44125)
                     /locus_tag="PKM27_01930"
     CDS             complement(43433..44125)
                     /locus_tag="PKM27_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06042.1"
                     /translation="MTRGIKSNLITYGFFLSVAAAGLVYGKSILPSFNHLRVWDVQTL
                     LLLLIGVPFLFFQTKAGLPDFWDSQISHRTRILLPVLIGMVFGILDVLVFKVLLHPEP
                     YTELPPFLQPFPYSLFLYFSGALEVEVFYRLIPLTLICAIGHGYKKGKYAHLFFWTAA
                     ILTSVREPLEQYSGGSALLIFYSFATGFTMNLFQAAWYKKAGFPATLFIRLGHYLLWH
                     ILLGIYVESVEL"
     gene            complement(44177..44509)
                     /locus_tag="PKM27_01935"
     CDS             complement(44177..44509)
                     /locus_tag="PKM27_01935"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNR06043.1"
                     /translation="MTEELKKEGFGILTEIDVQSTFKKKLDVDFKKYKILGACNPQFA
                     FKSISSEDKIGVFLPCNVIVEENPGGEVEVSAVDPVASMMSVENEALGDVAADVRQKL
                     MKVIENLN"
     gene            44850..45797
                     /locus_tag="PKM27_01940"
     CDS             44850..45797
                     /locus_tag="PKM27_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06044.1"
                     /translation="MKAFLPDGKTLDIKALDQEGRIYDVKALQQGDNLHMMDIKALFN
                     GTTLPVKMLVSDEKYAPVKAIGEDGTLYDIKALTGDGQRLDVKGINLSGNVISLKAIN
                     QAGEYYGVKALSRNGQLYDVKGIKLSSDLVEGRVNGVDFYAHIKALPQIGQEGQDSIW
                     HIKAIHPEGRILDVKALDKEGRIHDVKAVIKDGDLHLIDVKAFIGGKQQPVKMLVSDE
                     SNIPVKAIGEDGTIYDIKALTAEGDKLDVKGIRTPGNIIQIKALGKEGALYGIKAISP
                     TGNLHDVKGVKVSGEEVELKLNGVEVYAHVKALPVVPDR"
     gene            complement(45830..46330)
                     /locus_tag="PKM27_01945"
     CDS             complement(45830..46330)
                     /locus_tag="PKM27_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNR06045.1"
                     /translation="MIFFISWSGCRSIPKGGTAIKPFEVDRYLGKWYEIARLDFRFER
                     NLNNTSATYTANPDGSIKVVNRGFNYKTQEWKEATGKAKFRGDKELAQLKVSFFGPFY
                     SGYNVIALDPEYKYALVAGKNLSYLWILSREITIPDEVKQNYLKIAKDLGFKTEELIW
                     VEHNKK"
     gene            46471..49428
                     /locus_tag="PKM27_01950"
     CDS             46471..49428
                     /locus_tag="PKM27_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNR06046.1"
                     /translation="MKRTRLSFMLRGKPSGDHRRQWMTFILVFSCFFITGQVSDPEAV
                     KSMISRFKQDARGPYRDIRWFCKDGSTRAARDPCTDQTGNQRARYKEEVDLLAARDHI
                     FLGQILATTPYTDIWDESSAHSRMIQYQLEQYLRSMDNGWIHRRAQFYRGAVQIEDEH
                     RWGAGFYQSLLEDTFSIHSRYFLLRQSARDIPHSGDDNTAQRIRSLCKEISDSLPSFM
                     DLRVKIHGAPDAGDIQKVNHFRQGIGKEMTAALNEKFDLLLRELTIMYQPFMVSDLED
                     YRRVLPETSPAKGLVTVFMNGYPLLAKPEQRCRLISRTAMMLRHQLASPMQGISRLAL
                     IDVSNKMENLIIIEASRWKAGSVRELISQVYCLSEAVAAFGYLELWEWEQVSSSLELP
                     AGDSISLNQLNALHEGSRRVLEWATGLFRAHYQPVVNRYRDFEPLAAGFLDDRVRSSV
                     LLHLGRAVSQLGEAWAREAGFSNSVLGIAGQSSIRGLNPGFALGELVVVTGTPEKEDL
                     SSSKIYVFHHPPGNLKPVAGILTVSEGNMVSHVQLLARNLGIPNAVISQENMESLKAF
                     HGTPVFYAVSQKGTVIIKPAAAMEPREKELFEQKKRKAEKISVPMDRIVLDQAQILNL
                     RQVSASESGKTCGPKAAHLGQLKQIFPGQVVEGLVLPFAVFKQHMDQRIPSYETSYWD
                     LMKGIFTTAERMRARGDPEAETEAFILKGLDSLRRLIRKMPLLYNFRKELEKQFQTVF
                     SQPLGKIPVFVRSDTNMEDLRDFSGAGLNLTVFNVLDSEKIYQGIRDVWASPYTERSF
                     RWRQHYLENPENVFPSILIIPSVDPDCSGVLITKGLSSGSEGDLTVAFNRGVAGAVEG
                     QVAETWLLDAEGKEHLVSPARELFYLTIPATGGSLKVRTGLEKRILTPERLGALRKIS
                     QHLIEELSASPEVNNRGPLDVELGFLGDKIWLFQVRPFVENKLAAATEYLRQITPAFD
                     GDRSVSLNSKLDE"
     gene            49425..50684
                     /locus_tag="PKM27_01955"
     CDS             49425..50684
                     /locus_tag="PKM27_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR06047.1"
                     /translation="MMVRCSVFCFVLICIFILEAASYPIDGYSHTHIRRLKYLQMVVD
                     GELKGTKPIPGAMKSYSDIRLHLTGERGDSLAVIPSPDADLQEKIHGLFPVLGENYSI
                     ALLDITPGRPLRYAGRKEHEGFQPGSVGKLAVLLGFFTELAKIYPDSFELRRHLLCNK
                     SVRAGGWALYDEHTVSIFDTETKVLKRRTVQAGDVFLLYEWLDYMMSVSNNGAATVCW
                     REAILMRVFGKEYPALSEVQAEAYFNKTPKSELSETAISVVNEPLRAMGITADEWRLG
                     TMFTRGGSGRIPPKGGSTGTPMGLMKFLIGLERGKLVDEESSLEMKKLLYMTDRRIRY
                     AASPALKDAAVYFKSGSLYKCRPEEGYVCQKYKGNVDNFMNSIAIVEHPDQTTYLVAL
                     MSNVRKKNSASDHMALAASIDRVVRGD"
     gene            complement(50687..53518)
                     /locus_tag="PKM27_01960"
     CDS             complement(50687..53518)
                     /locus_tag="PKM27_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR06048.1"
                     /translation="MLEALLSKWRDFHLRIFDIREGEFRRVWLMQLTIFLIIQSLWII
                     KPVVNAQFLSRAGIEKLPLVFLLVALTALVVASAYSRWLNRIPLGALITRTHLISIFC
                     LLTFAILLRLDLFKDWMSYLIYIGVALFGLITTSQFWLLGNMVFSSLEAKRLFGIIGA
                     GAIAGGIVGGYVTSLLASVSDSINLLFYAAGLLIISMAVNRRIWSRFIPEYNRSFQLR
                     QTKALHEYPLRLIRNSKHLTFLALIMGISVVVAKLVEFQYSAIASSKIRDPDQLTAFF
                     GFWFSTANMVSLVIQLLITRKVVEYLGVGRSLFVLPGALFAGAATVFYTPVLWTGTLL
                     KLTDISLKQSINKAATELLIMPVPPAIKNQVKTFIDVFVDTTATGIGGITLIFLINGL
                     DLSVRAVCIMIILLAGLWIYFTILVKKEYVLAFQKILGLNPNISAKKNFQLSDETVAD
                     GIKRTLQSGSVHQIQFLLERLEEIKGHSIMPEVIPLLTHKSPEIRQAALRCLYYHTDH
                     SVINHIEPLLKDPNDEVRARAFSCLLAHTRDRRVHFIEESLKDPDPAINRAALVGLAT
                     EARDNLEMQQQFNLEQRLRETFEHSARFPDPKEQEAQKMMVARAIGYGKLTSLYSVLA
                     DLMKDANPAVSGKAILAAGYSQDPVFVKILLAFLPDKSTRSVAQKALAQFEPHQILPI
                     LTDVSIEKSTLREILIQLPALAKTMDTQQAIDFLFELVQHRDPLVKLKAIQTLHKMKV
                     NFPHLTISGKRVVPHLLREAGLYRDALAINYAAQHQWKTDQESEGIILARKEFTRLLE
                     RKLNRILKRIFWLLGLTYPPGIILPLFKDIRNSDANIRINTVELLDNIIDPSLKKVVI
                     PLVETAALEHLPDEALKRLQLKSPDEYACFESLLKGPEVRLKLAVLNLIEAMDNPEFD
                     PLIHLATGDEHYRVRLLAERLSEIRRLDV"
     gene            complement(53532..54527)
                     /locus_tag="PKM27_01965"
     CDS             complement(53532..54527)
                     /locus_tag="PKM27_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR06049.1"
                     /translation="MKKFTAYSLLISMSSILIVFFFTLTSWSSVPSTSLPGNPVGKAE
                     AVANPGHPVLDHLPLPVPEGVVKPLEALKDQELQDQLETILNSDRRWMELARNKSLSI
                     GVVDMYDPMNSRFAAINGNHMMYAASLPKIAVLLASEEAIAQGTLQETPEVKQDMKWM
                     IAKSSNEAASRMIQRVGIGQIARVLQHPSYKLYDKDNGGGLWVGKPYGGGGTRMGDPL
                     KNLSHAASVEQVCKYYYKLAFGQLVNPERSRDMLQILVDPQLHHKFVSVLDRVAPKAK
                     VYRKSGTWENWHADSALVWDDSRRYIVVALAMDSQGEKLLRDLMLKIDSALVANG"
     gene            54780..56120
                     /locus_tag="PKM27_01970"
     CDS             54780..56120
                     /locus_tag="PKM27_01970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNR06050.1"
                     /translation="MSNSHMTGLKRHSLRMYVFLLLTATNPPFTNGQTSISKWQFGKQ
                     GAVSITYDDGSINQFRKALPIMNHLKLPATFFIITGQIPGSQYQGKFIGRPVSEIIEE
                     SSSVLTNKDNFFERASAAGFLGYRGTLAYHTRAGALYDAGKEEEAYAILDDLYQKVRN
                     QEFLPDTGTRYGEIPADRVTWDEIRSFAAQGHEFASHTVTHPYLCALDEVNINYELEK
                     SRDEIQRQLGPEYTFSAEIPYGTEHERAMKYAHRVFPALRNRMPEHYLVELNRASTKN
                     PVDFTEEYVQWQRGAVARTSLPMMKSWIDTTLLKDNIWLVLVIHGVDGIGWEALSSEV
                     LDEYFRYLKSKENHLWIATFGEVTKYMRSRMNARVQSKSQDGKITVSLTHSLDKSLYQ
                     VPLTLKTYVPGHWNEVRIKQGNEIKRIRQQLDSAGAYVLYQAYPNSEEIELTAN"
     gene            56165..57376
                     /locus_tag="PKM27_01975"
     CDS             56165..57376
                     /locus_tag="PKM27_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNR06051.1"
                     /translation="MAESNNPSRVSGPPRIASIDILRALTMVLMIFVNDLWSLKSIPA
                     WLGHVEQGVDGIGLADVVFPAFLFIVGMSLPFAIDNRRKKGDTGIQITGHILLRTISL
                     LVMGVFLVNGETFNAAATGMARYLWNPICCLSFILIWNAYPPTTGKALKYGLKSIGMI
                     ALLVLAFIYRGGTEGDIYRFSPQWWGILGLIGWAYLVSALVVLWSKNRLAYLLLAWTF
                     FALLSIAAKAKWFSIDALSFIPGAISGGTLTGLALGGVVASQVFRYYRERRDDRTMTY
                     LFGAAIVILVALSVLTRPYWGLAKLGATPAWLFLCSAFTLGAFVIIYWISDVYGKAAW
                     FNLIRPAGTDTLLCYLMPYFVYFILRVLHVRWPEVLVTGGVGLVKSFLLALLCVWLTR
                     ILNGVGVKLKL"
     gene            complement(57496..58485)
                     /locus_tag="PKM27_01980"
     CDS             complement(57496..58485)
                     /locus_tag="PKM27_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNR06052.1"
                     /translation="MINTESNAVGVAGLGYMGCSIAAAFLIWGYKVVGLAGLESDLDT
                     APGRIRHSLDEARKQGIHDRNVEDLLSGISYTKHHADLEPCRLISENVLEDLEVKKSQ
                     LALIEEAVSAETIITTNTSAIPITDLQANLKIPGRFLGMHWAEPAFTSPYLEIICGPD
                     TDPSVAESLYLLASTWGKEPTLLRKDIRGFITNRLMYAMYREALYLVANGYTGLEDID
                     RACRNDAGRWMTFCGPFRYMDLTGFQAYYHVMKDLFPELSNLSEVPPFMEKIVQKGGK
                     GIFNGHGFYEYSREEAEAWAKAFEEFRFAVHDFSKQYPVDLVQQRLGKDIKEK"
     gene            complement(58593..60299)
                     /locus_tag="PKM27_01985"
     CDS             complement(58593..60299)
                     /locus_tag="PKM27_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06053.1"
                     /translation="MILHAGPVQMLYESGFLRYITYGETEILRMIYFALRDENWATLS
                     LQTEQEVTDIQADHFTIEYDAYHEKDGRRIFQWRVSISGFADGSLEFMIRGRALTDLL
                     KNRAGFCILHPISAARQPCKILHPTGAVSKSFFPDQVDPVNPFKLIRGFQWEAGGRWY
                     SLDFEGDLFETEDQRNWSDASFKTFCTPLSLPFPVKLHTGETVFQKVQFKPLETLAAL
                     RTPDPTIKIRSTHRRFPTPRIGTGASTEIGSLDPETLAVLRSLQLDHYRIDLNLSRPA
                     WEAEGLREYQNSVSLGCSPYFALHLTAPCLHQIERFIGFCHSHRIRPQYILLLSEDHP
                     VTSPDALSCLPVIRSAFTEAKTGAGTDFNFTEINRHRMETSGLDFVSFSVNPQVHAFD
                     DRTLIENTGSLEELVLSAKHKFGANMGIHISPITLRMRRNPNATDPEKRNFTNQQKTD
                     PRQKTFLGAAYALGSLKRLALGGCEAVTLYQSAGNQGLLSAEGEAYPVFHALKTWLGS
                     CGELIETLSSQPLILDALLLDKEGRKKLILINYANTTQVAEFEGALFTLSPHEIRTLD
                     CP"
     gene            60432..61628
                     /locus_tag="PKM27_01990"
     CDS             60432..61628
                     /locus_tag="PKM27_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNR06054.1"
                     /translation="MKLKSILAVFLFFSTFSIPAQEIGIIMGTARHIMQEDVEQGLIR
                     LKELGIRHLEGAGSRTMSREEYKKLLDKHGFDVVANGVNFERLEHPDSIKRIIDNMKF
                     YEAEYAVCYWIPHKGDDFTFADMQKGVEVFNRAGKQFAESGISLLYHPHGYEFRPYDG
                     PGTMFDYMMEKTDPRYINFEMDVFWIRNPGQNPAALLRKYPGRFPLTHLKDRLPGSVD
                     NPNGRQDPQRNVVLGSGDVNIAEVMKAARETGVKYHLIEDESARAGVQLPMHLKYLRS
                     LDLDMQAVELSVSSLRKAMLSADSLALRNLTCEELTYGHSGGLIEGRETFVNNLVTKK
                     TDFITMDLKDQEITVKGDVAWVRHLLSAEIVDNGNKNNVNLKILLIWTKVDGIWKLLA
                     RQAVRA"
     gene            complement(61699..63996)
                     /locus_tag="PKM27_01995"
     CDS             complement(61699..63996)
                     /locus_tag="PKM27_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06055.1"
                     /translation="MKIICFTCLIIYSLPGFCKTWFVTNSGNSFSPATITISPGDSVQ
                     FNLAVAHNVREVSQGTWNANGTSSLAGGFQLPFGGGLVLPAQLGAGTHYYVCVPHASQ
                     GMKGTIVVQGCSPPSTPGLITGNNTVCSGSPNTYSVGPVNGATSYTWSLPGGWSGTSV
                     TNTINTFAGPNNGNITVTANNACGPSQARILTVTVNQSPSTPGLISGNTSICSGSNNI
                     FSVNTVSGANSYTWVLPGGWTGSSMTNTISTTAGTGGGTITVMANNACGSSSVRSLAI
                     TVNSPPATPGTINGNTTICSGSTNTYNIGTISGATSYAWVLPDGWTGNSMTSSITVTA
                     GTSGGNISVTANNACGLSQARNLVVTVTPSPGMPGSISGDTAVCQGNAKTYTIAAVTG
                     ADSYNWTLPAGWSGSSSSTSINVVPGPTGGNITVSANAACGASAIRILAVAVRSTPAV
                     PGPINGDTSVCPGSSLTYSIQPVNGATAYEWSIPFGWSGNSIGTSISLITGNTSGILT
                     VAAMNACGSSSFRPLLVRANSIPSLPGPITGDTNVCSGSIHAYQITPANGATDYTWIV
                     PAGWQGQSHSSIINLVAGTSSGHLMVFASNLCGSSETKMLPVNALSLDTAVLQTNGLL
                     MARDTTAAYQWVSCPGLVPLEGQSSVSFMPEDHGLYAVILSKNGCVDTSSCLGRLTVS
                     NRDVPENKKIKIWPVPGRGIFQIQTDGALLSGRTSIEITNLQGKTVFKGPLTQSIDLS
                     TQPDGIYFIRVFYGNQVRVNKIIKE"
     gene            complement(64305..64736)
                     /locus_tag="PKM27_02000"
     CDS             complement(64305..64736)
                     /locus_tag="PKM27_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HNR06056.1"
                     /translation="MKNMETQLLREEKVYPSAEVLGKALGKPFVLFKAFIQTVTGEKY
                     HLEPKWNYYKDGKAWLCKVCVKSRTVCWLSIWDTGFKTSFFFTSKTISGVAALDIAQK
                     IKNELGTLKPIGKLVPLVIAVNNKKKLMDVLKIMEYKMSIA"
     gene            complement(64758..65852)
                     /locus_tag="PKM27_02005"
     CDS             complement(64758..65852)
                     /locus_tag="PKM27_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNR06057.1"
                     /translation="MKKNNVQTEVDACFLYLKLAEHESDPTLVNVFRQMSEIEKSHAE
                     AFAKKENLPFESLLRPSWRAKTMNVIGKVFGYDYVLGFLMDTEKNLSNAVISTKQKNK
                     QAITGMETTHVTILRSILEKEKNVTGAQLSKMEKSHRSVGGNAIRAAVLGGNDGLVSN
                     FSLIMGIAGATSGQEGVLLAGLAGLLAGALSMALGEWISVRSSQELYENQMKLEMEEL
                     ETNPEGEMKELALIYMAKGIPEEQAHHMASELLKNKDLAHETLVREELGINAEELKGS
                     AVEAALYSFILFALGAIVPVIPFMFSSGMPAIVLSVTASAAGLFLIGAAITLFTGRNV
                     WFSGFRQVLFGLAAATVTFGIGKLIGISMG"
     gene            complement(66056..66583)
                     /locus_tag="PKM27_02010"
     CDS             complement(66056..66583)
                     /locus_tag="PKM27_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR06058.1"
                     /translation="MMNFDLNKSVEILSRTPVLLEALLGGLSEEWIYNNEGEMTWSPF
                     DVVGHLIHGDRTDWIPRMEIILSGGPDRKFEPFDRFAQFEASKGKNIRQLLDEFQSLR
                     SNNLRILKSKNLDTRELAKTGIHPEFGEVRLDQLLATWTTHDLSHIAQIARVMARQYT
                     REVGPWIQYLPILNR"
     gene            complement(66642..68180)
                     /locus_tag="PKM27_02015"
     CDS             complement(66642..68180)
                     /locus_tag="PKM27_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNR06059.1"
                     /translation="MKKFNFLWMAMWFFHPSIGQEANKGGQWPPLVTMTAQEDHDDMM
                     RQLGIKKLRPGPSGNESAPNHANYDESKANPCPQLPEILTTRSGQKVSSAEQWWKQRR
                     PEIVEDFEKEVYGRLPEHIPAVTWTVKITDREFVGRVPVIAKQVIGHVDNSAYPLIQV
                     DINMILVVPANVKGPVPVLMMFGRPAFPSPAQPPAEDMEKLNAVFKEMMIKADPSLKA
                     IFDRYPAYSPVTRLPAPNFFAPPPAGDLPPTEQLLAAGWGYATIDPASIQADNGAGLT
                     RGIIGLVNKGQARKPEDWGALRAWAWGAARGLDYLETDTLVDAKKVGIEGVSRYGKAA
                     LVTLAFEPRFATGLIGSSGKGGATLHRRVFGEAVENLTGGLYYWMAGNYMKYGAAEAD
                     FGSKTGCDLSVDSHELLALCAPRPVFVSYGIPEKGDARWLDQQGSYMATVAAGPVYKL
                     LGARDLGVSHEYRTATMPPVLEGLLDGELAWRQHDGGHTDAPDFKYFIPWANKLWKYE
                     RAAQ"
     gene            complement(68207..70174)
                     /locus_tag="PKM27_02020"
     CDS             complement(68207..70174)
                     /locus_tag="PKM27_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNR06060.1"
                     /translation="MTRMPWSVNRRPFLFYLTLLLNPAPSFTQIRLPQIIRDSMILQR
                     DQPIPLWGWASAGEKVSIIFDGRRHETTASPEGKWKLVLPAMKAGGPHLIKISGKNDI
                     TLKDILFGDVWFCSGQSNMVHQMNIHDVTYAHDIASANQPEIRQFLIPTRTSLSGPNE
                     DLSAGSWRPAVREEVRPFSAVAYFFALKLYEKYRIPIGLINASVGGTPIEAWTSEEGF
                     REFPQVLQTINKNKDTAYIHEMNRRAAPPNNPAARPSQDKGLTGPKTWFAEDYLPSAW
                     RTIHIPGYWEDQGVKDLNGVVWYRREIELPTAMAGKAARVWLGRIVDADILYINGKQV
                     GQVTYQYPQRRYSVPAGLLKAGKNTFVIRVTNFNGKGGFVPDKPYCIFTANDTVDLKG
                     DWKYKVGEVFIPRPPSPGGINAQNQPTALYNAMVAPVAHYPIKGILWYQGETNAGRPK
                     EYAGFLPTLIRDWRNQFNQPEAPFIYAQLPNYMDYNYLPSESNWAELRESQTKALSLP
                     NTAMTVNIDLGEWNDIHPDNKKDVGERMALAALKIAYGESLVHSGPLYHSHAIEGDKI
                     RITFTQVGSGLISLDGEELSDFAIAGEDKKFVWAKAVIRGNELMVWNQEVPNPRHVRY
                     AWADNPVNPNLYNREGLPASPFRTDQPVSEK"
     gene            complement(70660..>71328)
                     /locus_tag="PKM27_02025"
     CDS             complement(70660..>71328)
                     /locus_tag="PKM27_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06061.1"
                     /translation="STLINQDINTGAIDSRTILDSTILNQDLGTGSIDSRAILDGTLN
                     NADIADSTIDLATKVTGLLPVSRGGTGTDTLTANSLIIGNDRGALRSLGKATDGQIPV
                     GVTDSLPKLKVLAGTDGIKVSVTPDSVIISSGIANIISTVADTIDPGIIFINTTWLSP
                     PIAIPGVDLGNIVVGSIPADLKGCQMTTFVSEPGMIKVSIYNGTFNNVNLGTNRILKV
                     MVVQ"
     assembly_gap    71329..71428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<71429..72215)
                     /locus_tag="PKM27_02030"
     CDS             complement(<71429..72215)
                     /locus_tag="PKM27_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06062.1"
                     /translation="MNKLFYISIFFSIGILPHALSQNKIGDLSGIYYQAVAVDETGKE
                     IVGTDISGKPVYKKAIGVRFTITKGLNGPVQWEETHTTTTDKYGMFTLIIGKGTVSGS
                     NPYPRMLDIPWIDADQFLKVELAIKNDGNYRLVSNQQFMSVPYSFYTDDIADNAITTY
                     KILDSAILNQDIHTAAVDSRTVLDSTLLNQDINTGAVDSRTVLDSTLINQDIKTGAVD
                     SRTVLDSTLINQDIKTGAVDSRTVLDSTLINQDINTGAIDSRTI"
     gene            complement(72246..72875)
                     /locus_tag="PKM27_02035"
     CDS             complement(72246..72875)
                     /locus_tag="PKM27_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNR06063.1"
                     /translation="MKTMKLFLLLCLFVAFSTVLPAQSGLVLEASQLFTSFKFKDRAG
                     VSHNKEYQGLYTGAYGIGYRYVNDIGLIIKPGIGMRNGGANLVYDDQNYSWKLQYADL
                     KLGLGYMTRFETVNPYFLASGYYAFLLRGLQVTHNENLNITESNLLSNIDYGLIFSPG
                     LNIKLSDYISTYLELNYLLGLTNIEKDPGQKTKNFGYGLSVGVSISFSE"
     gene            complement(72884..73399)
                     /locus_tag="PKM27_02040"
     CDS             complement(72884..73399)
                     /locus_tag="PKM27_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06064.1"
                     /translation="MNQILVMKKWSMAGLFVMAFILPGTAWTQSIKRQSISSYGSSGL
                     TENILIGQTAGQSFHTELGSIGVTVAQGFQQPVLFTVKEISNPFLNNLNVVVFPNPAS
                     RSVTLASEEETGPSVITVSDVNGRFILSEKVPNLSSHTIHCASWANGMYLITIHDSKQ
                     NSKTLKLIISN"
     gene            complement(73855..74232)
                     /locus_tag="PKM27_02045"
     CDS             complement(73855..74232)
                     /locus_tag="PKM27_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06065.1"
                     /translation="MIKTAAVCLWFAGLGFGIPCLYGIWSLMKGKGIPYVLGFPSYGY
                     GPFERMGIHTTLPLLSGFLLICVVECVAGYGLWNGDKGSAVLSLAIVPVELLFYYGFA
                     LPFGPPVLVLRLILLFLSWSSLN"
BASE COUNT        19033 a        17761 c        18893 g        18897 t
ORIGIN      
        1 atcattggtg agtttggcgt cgggtccctt gaagggtcgt attatagagc ggccttaaaa
       61 tctggcttcg ttatagaagt ctttgaaccc ctgaaagaac agaaagcctt tctaaaaggt
      121 ggatcggccg gacaattgat acacaaattt ttaccagtcg atgcctggag caaaaagatg
      181 aacagaaaat gggtctccag ggccatttca tttggacccg atgtgatttt atgttttact
      241 aataccaggg ttttgccggg ggcattggcc agcatcaaag ccttgcgacc aaaggttaaa
      301 ttggtttgga tctggccgga tcctcctctt agcctggaaa cccatacgat tttaaatggg
      361 cgattggttg atctcaccgc tactttcagt tcatccagtg ttgcggtttt ggataaattg
      421 ggtttcttca atgtccgttg gattcccctt gcggcagatc ctgatttgca tgcaatggaa
      481 gttaggagcc aagggttttt gcgggacatc ggatttgttg gggggtggcg gccggaaaga
      541 gaggaggttt taagcgcaat ttgcgaggcc tttccggatc tcgtcattga gattcacgga
      601 ccttactgga aaagggactg caaaaacagg caggtaagga aaagagtaaa aggaccaggg
      661 ctttatgaaa aggacctggc cacctttttc aatactacaa aagtaaacct gaatgtgatg
      721 gatcaaaccc attttatgtc tgccaacatg agattttttg aaattcctgt cgccggggga
      781 ctccagctca gttccgcatg cccggaaatg gaaggttcat tccataatat gaagcatgtt
      841 atctattttt cagaggttca taaaatcacg gaacaattaa aatggatttt taaccacccg
      901 ctggaagcgg ataaggtccg aagggctgga aataaaatgg taatgggtaa gcacacttat
      961 atccaaaggt ttgacttgat tttagagcac ctcggattaa aacaggataa gacttgattc
     1021 atggggaaga gtgaggtcag cctgaccgaa gcaaaaggat ttataggaca ttcagcacaa
     1081 ccgcagattc tgttgaatga tgtgttgaaa tttccggtgt cccttttatt gctcttattg
     1141 ctgggaattt attggattac cggaaacgaa gtgccggtta ttttctcatt agctgtactg
     1201 ttgtttttat acatgatctc aggtttgggc cataaactca tcattcctga agcgatggcc
     1261 ttttatatca ttatgatata ccttcttttc cctctatggg gatatcaaag gtatcccgcc
     1321 caacatcccc tttccacctt gttcgatgtt tatatgaggg tgcccaggga ggaatatttt
     1381 gaatttgcta ttcctgccgt tattcttttt atttcagcca tgttcaatgc gcagcgaaac
     1441 aatgatatct catttacaag ctccctgttt accagcgcca ggaccaggtt ggctggggtc
     1501 caacaggtgg gtgtggtttt gattgtaacc gcattgatgg cctatttcct gggccctcgg
     1561 atgccttccg gtttacaata tgtattgcag cttggtttcc tggcgatcat ccccggcctc
     1621 ttctatttat actttcaacc tgggcgtggc ctgggaaaaa ctgtgatggt cctgacgggc
     1681 ctggcgtggt tgttttatct ggccattaaa tccactatgt ttaccctgat cgtctatatg
     1741 ggcatcgcct ccctgggttt cctttttatt ggaaggaaga tcagcatggc tggtaaagtg
     1801 gctgtattct caatactctt gatcgttggg ctggttatgc agtacaccaa ggccaactac
     1861 cggaatttga ttagagtgaa taaggccgaa agcggggata tccgggtatt cagcaggttg
     1921 tttttgcaaa atctggtgaa cattgatcag gcggtattgg acggggcatt ttttcctatt
     1981 tatatgcgag gaaaccaggg atatcttttg tccagggtca tggagcatgt acccggtcgc
     2041 aaacctcacg atggaggaag gttattaggc aaaaccctgg tttcaagttt tattccgcgt
     2101 ttgttttggc ccaataaacc tgaagccggg ggtaggttta acatcgaata ttatacctct
     2161 ttgaaattaa gccggcggac ttccatgaat gtcggcccga tcggtgaagg ttatggcgca
     2221 ttcggcaaat ggggaggtat gctctatatg atttttttcg ggaccgcact ggggctggcc
     2281 aaccgggtat ttctcaacac ctgcacaaag cttcccctgt tattgttctg gcaaccgctt
     2341 gtcttttatg aggtgatata ttgtatggag aacgacagca tgcaggcatt taattcgctt
     2401 ataaaaattg cctttttgtt atttatcctt aataaaattt tcccgaaact tttcaagggc
     2461 aagccattgg attcctatgc ggggtatggg gatggagggt tcccaaaact tcaacgattg
     2521 gattgagcag gttttataat tttcttatcc gttcagaccg cgttatactg gtatacggat
     2581 tggttatcct ttgtgcgacc tgtaggatct cccgacccgc gcttttaccg gaaggggacg
     2641 gaaagaccgg gcaggccatt gggttggaat ttttccctaa tgctgatcaa ccggatcggg
     2701 atattaccca tgaattgcag gaagcgatgg atggactttc ggtttaccca agaggcttta
     2761 accagggtaa gaaatcctat tccaccgttc atttgaaaag aggtaaatat tttatttccg
     2821 aaaccctgat cctccgtgga aagatcgggt ttcggatcat cggtgaagat ccgcaacata
     2881 ccattttgga atggagggga aaagaaaagg ataccatgct ttgggctgac ggttcggctt
     2941 actttaaggt atcaaacctg acttttcacg gtggatttcg gaccggcatg gaagggattg
     3001 gtattcattg ggccacccgg gcttcaggag gggtaaacca tttcgcatct ttaaatattg
     3061 aaattaataa ctgccggttt accgggggca tggacgttgg cgtggggggc gggtcttatg
     3121 cttcagggga ttggaccgga gcaaacgaca gcgaggtgac catccggtat tgcgaatttg
     3181 acggttgtgc ctccgcggga atcaggattg ccggattcaa tgcactggat tattggatat
     3241 ggaattgcag attcatgaat tgtgttatag gagtggactg caggcatggg aattaccatg
     3301 tgtacaattc ttatttcaga caatccgctc tggcagatgt gcacaacacg ggaggatatt
     3361 atacttcttt gagaggatgt ttttctgaaa acagcatggc cttctccgtt gatgggggga
     3421 gcagcagcaa tccgtttaag cgtgtttttg aaaacaatat tgtgaaacaa cctaaggaat
     3481 tgcccattca atatcatcat attgggaaat taagtcttta cggtaaccgg attgaccgct
     3541 cccaggcaga taccctgaac cggaagaatc cagtaaggag cccggacccc caaatggatc
     3601 cttccccggt ttccgtcacc tacggcagct ggtatccttc cattttcacg attttatcct
     3661 tgaggaataa attcttttat gctgccccct ttaggttttc atcggcacac ccgagcatgc
     3721 tttatcaggc ggacgataaa gtgggtcata ccgtttcctc ggagggactt gagtcttttg
     3781 aaaaaacagg aattcaggcc gttccagtcc taaaagaaac cgtattccgg gtgccgttaa
     3841 attcaacaac ggatgaactt caggcgatta tacaaagggc atctgcatcc ggtatagaaa
     3901 atccggtggt tcagtttgaa tatggaaggt atgccttaaa caaatctgtt ttcattcctg
     3961 cccacagccg aatcaaacta aggggagaag gactgattta ctcaagcatg ttggtaatcg
     4021 agaatcccga gatctttaaa ggggaggccc tgtttattgt ccgtggtccg acaaatgtca
     4081 gcattcagga tttacatata gtcgggaata caactcgagg agggagcaaa ctcattggaa
     4141 ttgatttcag gaatgtagat accaggaatg cgcgggtggt catggatcag ctgtactgcc
     4201 ccgctgaaac cagcgtcttt gtagaccgtt acaattatac tttatttgaa aagaacaact
     4261 cctttttcag cgatggcaat atcatcattg ggggagatga acaaaaagca ggcaggggca
     4321 ccttaaggtt ccatgcattt ggagggcaat atgccggaac gcaggttttc aacggggcca
     4381 gggcggtatt taaagattgc tggtatgaag ggagccgcaa aattccgtta agcctggttg
     4441 gggacgggga gatcctgatt gatggagcga tgtgcgctcc gaataaactg gacagtacga
     4501 ccacgattgc cattgaaggt ttccggggaa aggtcacact ggccaatatg tatatgtatg
     4561 ggtcgattct tctaaaacca tccatggact caaagatttt cctttggaat ataaacatga
     4621 accaggtggc caggccaagc ctatttgcac aagaaactta tccaggtcaa gtggccatcg
     4681 cgggcatcac tacggactgc aatttccctg gggagctgga ttgtggcggg tttaaatcct
     4741 atgaagaccg cttccataat atttccgata agaatgcttt cattttatcc aatatggagc
     4801 ccggtatgaa tgtaatgccg gttgaaggat tattgcctgg tgatttaaac cagatctccg
     4861 tttcgagagt ttccatggaa gggatgggga ttggtttgaa attttctaag tgaatagaag
     4921 ttttcttttt ttgatttgcg caggttgttg attttcaccg actggtaccc acctgcatat
     4981 aaggccggag gaccagtcca gtccgtacat ttaatgtccc ggcagcttgg aggtactttt
     5041 caccagagaa tcgtgaccag ggcttatgat ttgggtgtgc atgcccttct tcagggaata
     5101 cggcctgatt cctggctcag tcaggaatca ggagtggaag tatattatga ttcattaaaa
     5161 cctggccgtt tgtcgcactt ggttcaccta ataagtgaat atgacccgga cgtggtctac
     5221 ctcaatagtt tatattcggt aaaatttacc ttctatccca tcctggctca tcggtggtta
     5281 aaacaccgtt ccaggatcat cgtggccccc aggggtatgc ttaaggaaag tgcgctgcgg
     5341 ttgaagttta ttaaaaagtt tctttttata aaagccgtaa gagggatgca gcttcaaaag
     5401 ggcattgagt ttcatgggac gaatgtcaag gagggggaag aaatccggaa agcattgggg
     5461 ggagtgcgcg tgcacatcat cgggaatctg gccaaggaaa ttaaatcgga atgggttcaa
     5521 atcggaaaat ccagaggtca attaaatatt attacggtat cgagggtaca ccccgtcaag
     5581 aacctccatt atttcctcac aattcttagg caactggcac aaccagttaa attcagcatt
     5641 atagggaatg tggaagacat ggaatacttt cggaaatgcc aggagatttt atctcaattg
     5701 ccggtacata tccatgtgga ttttctcggg gacattcctc atcatgaaat atctccgagg
     5761 ttggagtcca atcatctttt tgtgctccct accttaggag aaaattttgg ccatgccatc
     5821 cttgaggcat tgtcatcagg gcgccccgtc ctcatcagcg accagacccc atggagaaac
     5881 ctggaggaag ctaaggcggg ttgggacttg cccctctcag atcccgggca gtggctcaag
     5941 aaattggaag aagctgcatc ctgggatcag gaagaattcg atgcctggtg ccggggagcc
     6001 caggaatatg ccagggtaca taccaataca acggagctga ttgaaaagta taaaaagatg
     6061 ttcagtgaat catgaaattt aaaagttaaa atagtgttcc actctgacct ctgacctctg
     6121 acctctgacc tctgacctct gacctctgac ctctgacttc gatgactatt cttggcctca
     6181 acgcgtatca tgccgattct tccgcagccc ttttcatcga tggccgtctg caggcagccg
     6241 ccgaagaaga acgtttcacc cggattaaac actgggccgg ctttcccatg gaatccatcc
     6301 gcttttgcct cagggacaag ggccagcaat taaaggactt ggattttatc accataggga
     6361 ggaatcctaa agccaaactt tggaaaaaaa tcctgctcct ggtgcggcag cccgccctct
     6421 tgctgaactt atctgatcgg gtaaaaaacc agtcggtaat ttttgatctg aaacaggccc
     6481 ttgcaggggt ggatggcagc ctcgacctgc tttcaataaa aaagcgcctg atgctgattg
     6541 aacaccaccg cagccatctg gcctctgctt tttatccctc cccttttgaa caggctgccg
     6601 tcctgtcttt ggacggatcc ggtgatttta ctaccaccat gagggctatt gggaaagatt
     6661 acaaactgca ggtcctggac agtgtggact ttccggtgtc ctgcgggctg ttctatacgg
     6721 cttttaccca attcctgggc tttccgaatt acggggatga gtataaggtg atgggcctcg
     6781 cctcctatgg acagccgagg tatgcagacc aggtgaaaaa attattgcgg tttcttccca
     6841 acgggctgtt tgactgggac caggcttatt ttgtacatcc aacacaagcc ggcttcaggt
     6901 atgaaaatca tgtgccccgg gtaggcgatc tttactccgg caaatttgta aagctgttcg
     6961 gccctcccag gcaaaaagaa gaagagctga gcgcttatca taaggacctg gcggcatcgg
     7021 tacagcgggt gacggaagaa cttatcttcc acatcgcctc agacctgaga aaaaagacaa
     7081 atttggacaa tctctgcctg gccggagggg tggctcagaa ttccgtggcc aatggtaaat
     7141 tgacggatgc gacaggattc aggcaagtct atatcccttc cgcggggcac gatgccggca
     7201 tttccatggg cagcgccatg tatgcctggc accatatcct tggccacaaa agaacagagc
     7261 cggtgcgcca tgcatacaac ggtgcacatt tttcgaacgc ggatatcgaa caattgctca
     7321 aaagcaagtc ggtggaattc cggaaatacg aggatgaaga gttgtacgat atcgtcacgg
     7381 ataaattgat cgagcccgga gtggtgggtt ggtttacggg aagggccgaa ttcggccccc
     7441 gcgccctggg tggccgatcc atcctggccg acccgcgcaa tccccatgcc aaggaattgc
     7501 tcaacctcaa aatcaaaagg agggaaagct tccgcccttt tgcaccgtcc atcctcaagg
     7561 aatacacctc ggaatatttt acccggaacg gagaggtgcc tttcatggaa aaggtatatc
     7621 ccataagaga agataaaaga gcgcttatcc ccgcagtcac ccacgtggac ggcagcggga
     7681 ggctccagac cgtggataaa aacaccgctc cgcgctatta caagctaatc gaacgatttc
     7741 ggcaaaaaac gggactgccc atcctgctaa atacctcttt taatgaaaac gaacccatcg
     7801 taaacacccc cggtgaggcg ctggattgtt ttttacggac gaggatggat atgctggtga
     7861 tggagaattg tgtggtaacc agaagttagc agctggaagt tagaagctag ggcgttttgg
     7921 tctggcattt ccggggattt agtagaccct ccccagcttc caggttagaa gcccggagtt
     7981 agaagctagg gcgttttggc ctggcagttc cggggggttt agtagaccct ccccagcttc
     8041 caacttctag cttccagctt ccaggttaga agctcggagt tagaagctag ggcgttttgg
     8101 cctggcattt ctggtgattt agtagaccct ccccagctcc caacttccag cttccaggtt
     8161 agaagcccgg agttagaagc tagggcgttt tggtctggca tttccggggg tttagtagac
     8221 cctccccagc ttccagcttc caacttccag cttccaggta gaagcccgga gttagaagat
     8281 ggggcgtttt ggtctggcag ttcctggggt ttagtagacc ctccccagct tccagcttct
     8341 agcttccaac ttctagcctc cagcttccag gttagaggct cgaagttaga agatagggcg
     8401 ttttagcctg gcagttcctg gggttttgtg gttcctcccc agctaccagc taccagctac
     8461 catctaccat cttcgagctt ccagcttcca gcttccaggt tacccgcttc actaaaaaag
     8521 tgacagaaga tcgaaaaatg aataagaaaa caacaggatg gccaggctcc atcgccccaa
     8581 cttccagctt ccaagttaga agctagggcg ttttggcctg gcatttccgg gggttttgtg
     8641 gttcctcccc agctaccagc taccagcttc gagcttccag cttccaattt ataacttccg
     8701 tttcacagtg accatttcca tcatcactcc ctccctgaac aatgaaacgg tcatcaggga
     8761 ctgccttgaa agcgtccgaa cccaggatta tccagaaatt gaacatctac tcctggatgg
     8821 tggatcgatg gacaggaccc tcgaaattgc aagatccttt cctcacctgg cagatatcct
     8881 ttccggagcg gatgaaggca tctatgacgc aatcaataaa ggaataaaca gagcggcggg
     8941 cgaagtgatc gctgtcctga atgccgacga cttctatctc cagccgggtg ttatttcccg
     9001 cgtgatgaaa tcctttaggg agggaaaagt ggactccgtt tatggagacc tcatctatgt
     9061 gagcaggaaa gacccatcca aaatcatccg cacctggaaa tccggcccct ttcaaaaaga
     9121 acttttttta caaggttgga tgccgccgca cccggctttc tttttgagaa aggactgtta
     9181 tttgaaatat ggtttgtacc ggaaggagtt gagcatttct gcagattatg aattgatgct
     9241 ccgaatgctt tatctccata gcctcccttg cgactacctg cccgaaaccc tggtggccat
     9301 gcgtacgggg ggcgcaagca accgctcttt caaggcccgc tggctggcta accggcagga
     9361 ccgtgctgcc tggacaatga atggattgaa gccggagtgg ttgacgctgt ggaaaaaacc
     9421 tctgagtaag ttgaagcagt atctctagag aaggagctcg gagttaggag ttaggagtta
     9481 gggcgttttg gcttggtggt ttcttggtca ttttgatgtt tcacagtaat aagggtgtct
     9541 cgctgaataa gaattctgct gtgcagccag gagccatcgc cccaacttcg agctcctaac
     9601 ttctaacttc catctcccta tctttacccc ttcatggaat accagatttc tgacctgtcg
     9661 ccacatttgt tctgggacgt ggatcgaacc ggagtggaat ggggaagaca tgagaaaatg
     9721 attgttgaaa aggtgttgat gtatgggttg ataagcgatt ggagaataat caatgccgtt
     9781 tatggaaagg aaaagataaa ggaaatcagt aaaacgatcc ggtccatgga tgaggtcact
     9841 ttgacctttt tatgcaccat atacgatctc aaaccggagg atttcagatg ttacagactc
     9901 agaccgtttc aaccgaactt ctgggaataa tcaggtacct tatgcaacat ccagacctta
     9961 aagaatttag gttggttggt ggcacggccc tggctttaca attcggccat aggaagtcaa
    10021 tggatataga cctgtttggc gacgctgaag tgtccggaga tcaaattttg gagcttttga
    10081 atcaatacgg gtttgtcgtc cctgtcacca gatcgaaaaa tatcaatatt tttcttttgg
    10141 atggtataaa aattgacttt gtaaagtaca gatatccatg gattgaggga tgcattgtgg
    10201 aggatgtcat tcgaatggcc tctcctgctg acatcggcgc catgaaaata aatgccatta
    10261 cgggcagggg aagcagaaag gattttattg atttgtatta tttgttgaaa aaatatactt
    10321 tattggagat ccttggtttt tttcaaacga agtatgaaga cggttcgatg tttttagctt
    10381 tgaaaagcct atgttatttt aaggacgcag accttcagca accccctttc ctatttgaga
    10441 atatttcctg gcatgatatg aaacaatcta tactaaacga atacaaaaaa attgtctgag
    10501 gcatgaaaaa agctttaatc acgggagtca ccggccagga cggagcctac ctttcccatt
    10561 ttttattacg aaaaggatat gaggtccatg gcattaaaag gagggcttcc ttgttcaata
    10621 ccgcccgcat cgatgatatt tatcaggacc cccatgtgga caatcagcgt tttgtgcttc
    10681 atcacggcga tctctccgac agcacaaaca tcatacgcat cgtccaggag gtccagccgg
    10741 acgaaatcta taatctgggc gctatgagcc acgtgcacgt cagttttgag gaaccggaat
    10801 attcagccga cgtggacggg ataggtacct tgcgggtact ggaagccatc cgcatcctgg
    10861 gcctggaaaa gaaaacgcgc atttaccagg cctctacctc agaactttat ggcctggtgc
    10921 aggaagtgcc ccagacggaa aaaaccccgt tttatccccg gtcgccttat ggagtagcca
    10981 aattgtatgc ttactggatc acggtcaatt atcgtgaagc ctataatatt tttgcctgca
    11041 atgggatttt gtttaaccat gaaagccccc tcaggggcga aaccttcgta acccgcaaaa
    11101 tcacccgggc ggccgccaaa atagccctgg gccttcagga taccctgtac ctgggcaatc
    11161 tcgatgcgca aagggattgg ggccatgcaa aggattatgt ggaactgatg tggaaaatcc
    11221 tacagtacca tcaggccgag gattgggtga ttgcgaccgg agtgactacc cgggtccgtg
    11281 actttgtacg tatggccttt gcggagttgg gtattgaatt ggagtttcag ggtgaaggaa
    11341 aagaagaaaa aggggtagtg atccgatcca acttgccgga attccagctc ccggcaggta
    11401 aatccgtggt ccgagtggat cccggatatt atcgccccac cgaagtggaa ctgctgatcg
    11461 gggactctac caaggcaagg accctgctcg actgggagcc ccgctatacg gttcaacaac
    11521 tttgtaaaga aatggtacag gcagatgtcg ccctgttcag aagggaccag ttgttgaagg
    11581 agtcggggtt gaagatcagg aatgaatttg agtgaaaggt ttctcgcaaa agcgcagagg
    11641 cgcttgaatt agattgctcg caaaggtgta tggattagtt tgctcgcaaa gtcgcaaagg
    11701 cgcaaagacg caaagaggtt tgattaaaat gtttagcgcc tttgtaccca aaaggataaa
    11761 aaatctgtgc gccttagcgc cttatcgaga aacaaaattc ttagcgcctt agcgtctttg
    11821 cgagaaataa attcaaaatt cttagcgcct tagcgtctta gcgagaaata aattctccgt
    11881 gccttagcga gaaacaaaat tcttagcgag aaaaatgact gaaaacgaat tagccaccat
    11941 tgtcgtggac ctttgttatc aaatccacac tcagctagga gcagggctat ttgaatctgt
    12001 ttatgaagaa gtattgtatt atgaattgat ccaaatggga tttaaggttg aaagacaaaa
    12061 gggaatcccg gtacgctgga aagataaaaa aatggatatt ggcttcaggg cagatttgat
    12121 tgttgatgat aagctgatca ttgaaattaa atcaatcgaa accgttttac cagtacataa
    12181 aagacaaatg cttacctatc tgaaactgac cggtatgaaa ttgggattac tggtcaactt
    12241 taatgaagcc ttgattaaag atggcattac cagaatcgtt aacaatctat aaatcagctt
    12301 tcaaactttg cgtcttcgcg cctttgcgag aaacaccatc aaactttgcg ccttcgcgcc
    12361 tttgcgaaaa acacatcaaa ctttgcgcct tcgagtcttt gcgagaaaca cgatcaaact
    12421 ttgcgccttc gcgtctttgc gagaaacacc atcaaacttt gcgccttcga gtctttgcga
    12481 gaaacatccc ctaatttctt ccctcctatg cactaaaaac tacctcttat ctttgcgcct
    12541 tcgcgccttt gcgagaaacc atcagaagga cctttgaagc cggattccaa aatatatata
    12601 gcaggccacc ggggaatggt cggtagcgcc atcctccgaa aacttaaaag cctgggttat
    12661 aacaacctgg tctacgcccc ttcctcagaa ctgaacctcc tggaccaggc tgccgtggat
    12721 gatttttaca ggcgggagaa accggagtac gtattcatgg cagccgccag ggttggaggg
    12781 attgccgcga acaacaccta ccgggcccag ttcctgtatg aaaacctgat gatgcaaaac
    12841 cacgtgatcc atggcgcctg gcagcatggg gtaaataaac ttctgtttct gggatcctcc
    12901 tgcatctatc ctaaactggc gccgcagccc ctgaaagaag agtatttact gacagggcct
    12961 ttggaaccga ccaatgagcc ctatgccgtt gccaaaatag caggcattaa aatgtgcgat
    13021 gcctaccggg cccagtacgg ttgccgtttt atttcagcca tgcccaccaa tctttatggc
    13081 ccgaatgaca attatgacct gaagaccagc catgtcttgc cggccttgct gcgcaagttt
    13141 attgaagcgg taaaatccgg ggcccctgag gtggttcttt ggggaacggg cacgccttac
    13201 cgggaattca tgcacgtgga cgattgcgcg gatggctgcg tctttctcat gctgcactat
    13261 gaccaggaag gccatatcaa tatcggcacc ggcctggacc ttcagatcaa agacctcgcg
    13321 gccctgattg ccgggattac cgggtacaag ggaaaaatag tacatgactc ctccaagccg
    13381 gatggtaccc cccgcaagct aatggatgtg agccggatca atcagatggg ctggaccgca
    13441 aaaatcaaac tcgaagacgg catcaaaatg gtattcgaag aaatcaagga tcaattttaa
    13501 accttggttt tttctcaaac ctcaaacctt ctactaatgt atcacgcaat aatcatggcc
    13561 ggcggagcag gaacccgctt ctggccgctc agcacagaat ccaaacccaa acaattcctg
    13621 gatatcctgg gtaccggaaa gacatttatt caaatgaccg tcgcgcgctt tgaaggctgg
    13681 atacctcctc aaaatatcta tatcgtcacc aatgaaaaat accggggcct catcaacgaa
    13741 caattgccgg accttccgga tgaccagatc atttgtgaac ccagccgcaa caatacggca
    13801 ccctgcatcc tgctggcagc cttgaaaatc agaaagagag accccgaagc cacctgtcta
    13861 tttgtaccct cagaccatct gatccgggat acggatgcat tccgggaaaa tgccctggaa
    13921 gccctggaat acgcctccag gcatgaggcc atcgtcacca tcggcattat accgacccgg
    13981 ccggagacgg gctacggtta tatcaaaatg gaagaaaacc aagacggctc agtgaaaaaa
    14041 gtcgaacagt ttgtggaaaa gcccaacctg gattcggcca aattttatct gagctccgga
    14101 aaatatctct ggaacgcggg tatgtttgcc tggcatgtca ataccattat ccgggagtat
    14161 cattcactgg ctccaggtat ttataagatt cttagccatg gtgaaagcca ttacaatacc
    14221 ggagcggaag aagctttcct ggccagccag tatcccctca ccgaaaatgt atccgtcgac
    14281 ttcgcaatca tggaaaaatc ggcgcacgtt tatacggtcc cggccggctt tgactggtca
    14341 gacctgggca tctggtcctc cgtctatgaa gccagtgata aagataaaca gggcaacgta
    14401 tgcattggcg gtaaactgtt gcaggaggag gccgccaaca acctggtcac ccttcctcaa
    14461 ggcaagcagg ccattatccg tggcctggaa gattttattg tgatcgacag cggggaggct
    14521 ttgttgattt acccgcggaa gaatgagcag gagattaaaa agagcgtagc ggcggtttaa
    14581 accaccgccg tcgttccgga gcatcaaaac atgcacatcc tcttcattcc ctcctggttc
    14641 gaaacacccc aacatccggt aaaaggtaag gcgatgaagg atctggctct tgccctagcc
    14701 agggaaggga tcaaagtcaa tgttctgttc cagactggcg agaaacttcc cccggtcagc
    14761 ctggcaggac ctggagtgga gatctggtat tcttttttcc cgggcagggg ccaattgtat
    14821 ccgatttgga atgcgggttc cctccggtcg tatttaggca cttttaaaaa atatttgact
    14881 ttgcacggcc ggcctgacct ggttcacatc cacagctatc ccgtacttgc catcgcacac
    14941 cttcttaaaa aaaaatacgg catcccattt ttgtataccg aacacagttc taaaattgcc
    15001 gagaagaaaa tcaatatttt ggaacaattt ctgatcaagc gatatcttgg aaaacaggcg
    15061 cccgtatttg cagtcagccg gtacctcaaa gaaagttatg ctgaatatat ccataacgac
    15121 atccacgtga tgcccaatac cattgatttt aaagtcttta aacctggccg gacggctttt
    15181 ccccggcacc tgatgatggt aaataccctc gaccaaaaca aacaagtggg tcttgggatc
    15241 aaagctttta ccaagtggca aaagggatac cccgacgcct gcctgcacat cattggtgat
    15301 ggcccggaac ggcacaacct ggaacagctg atacatcagc ttggcatgaa ggggaagatc
    15361 ctgctttggg gagaaaagaa ggtggaggac tggttgccct tattgcaatc cgcatcctgt
    15421 tttctcctga tgagccggtc cgaatctttc ggagtagccg ccctggaagc tcttgcctgc
    15481 cgggtgcctg tagttgccct ccagaacagg gggattgacg aattccccca tgtacccggc
    15541 cttcaaattc tgccgctttt ttccaatgaa ttggatatca gtcaaaggat tgatcaggcc
    15601 attagggaat tcgataaggg gcaaatgggg ctgacccgtg ctgagctcct ggcccggttt
    15661 gattttcctg tggtggccgg caaatacctg gaaatttata aacagttcct gaagcctttg
    15721 gcctgagacc ggtgacaaaa gtcaaaatcc tattcatttc ctattattat caccagcaaa
    15781 cgctgacggc tacccggaat tattatttaa gcaaggcttt ggccgatgcc gggtatgaag
    15841 ttcatgttat gggcaggatg gcgtccgacc tggtcttgcc ggataagatt aaaatgcatc
    15901 cggtatctgc ctgggattat cgcaggatcc tgatgttttt tggagtgagg aatggagtga
    15961 cttcctccgt cgttcgtccg ggccgctgga tccgttgggg atacgaattg ctgctgactc
    16021 atcccttcaa caaatggctg gccgaaggag ggggctttta tttgaataag gccaggcgta
    16081 ccggattgga gattctcggg cgccacggca tcacccatct cttttcttcc tatcgcccca
    16141 tggcggacca ttttatcgcg caggacctca aacataaaca tcccggcctg gtctggatcg
    16201 gggatttcag ggatgtgctt tggatggaga aggaaaaccc tcattaccag gaaaaatgga
    16261 tccggtccct gatccgggat atggatcacc tgactgcggt gacccgtggt gtggcagaat
    16321 tctgggccag tgtttactgc aaaaaggcgg aggtcattta caatggactt cccggtaagg
    16381 cggtgcaggc agtgccgcgg cagagcccat ctgaaaaatt catcatcaac tacacgggca
    16441 tgatctatac cctcctccag ggggctgggg tattttttga cgtattaaga gaattgtgtg
    16501 caacggaaga ccggttttcg gaatcgcttt cgattcagta ttccggtttg catgccaact
    16561 attggcgaac ctggatgaaa cagcatgggt tactggactg ggccagcatc cgtcatcagc
    16621 tgccggtgaa cgaagccctt gaaaaacaaa ccaacgccca gatcaacctc gtcctcacct
    16681 gggccaccct cgacgtcaag ggaatcatcc atgggaaatt caacgaatac ctgatggcgc
    16741 gcaaacccat cctctgcctg attgaagggg accgggatac ggaacttgaa ggaatatacc
    16801 gcaagttgcc caatagtctt cttgcctata accgggagga ggacaaaaac ctgatccggc
    16861 agtttattct ccggcattac aggcaatgga ggcaaaacgg aagggtggag gaaattcccc
    16921 aggaagtcct ggattcctac gactggtcaa aacagatcaa ggggattgaa gcatacctgt
    16981 ccaaaagctt taattcttta attctttaat tatttattta aagttgaccg tactgatcgt
    17041 caccttcacc tatcctccgg ccatgagcgt caatgggttc aggccccggt attttgctaa
    17101 agccatggcg gaacagggct ggaaagtccg ggttttgacc aggcatttca gcggaaagga
    17161 aaccagcgcg gaagaatacc gcaagccctt gagcacggca ttttccatca gtgaagaggg
    17221 tgcggtgcat gtgtacagga ccccctttaa aaattcctgg tttacgtatt atgaaaaggg
    17281 gttcctgagg cggaccggtt tatggaaggc ggtttacctg atccaattgc ttgccgggcg
    17341 cacctgccag gaatcctata atgcctattt caggaaatac ctggcccagg tactggaaac
    17401 caataaaccg gatatcatcc tggtagagtc cgggccgact aacctggtcc ggctggtttc
    17461 taaaaccgct gtaaaatacg ggatgcccta tgtcattgat ttcagggatc tttattatca
    17521 tgaaatgtac cgtgatttcg gccttttgcc atttaataaa aaggttaaga ttctgttgga
    17581 gaaatggtat atgcgcagaa gcataacatc ggctcagttg gttttcagtc ttaacaaaga
    17641 gtggttggat atactccata ttcccacgga taaaagatta atgatctcca atggattcga
    17701 cagtgaaata tggaatgaga tacctcctca aaaggagttt gaagatttta ccatttccat
    17761 tatcggaacc ttgtacccaa gggaattcat acatgccttc ctgggctccc taaatgtgtt
    17821 ccttcaaaat cgtccggaaa aggtaaaagt aaaatttatt gcaccaggat caaaggcgtt
    17881 ggtaaatctt atcagggaaa cccttccatt tgaacaggtg gaggttgttg aatcaagatt
    17941 gccgtatagg gaagccattg tgatcatggc gaaatctcaa attttgatgt atcacgggtg
    18001 gccggggtac aaatgttaca ttagcaccaa agtctatgat tatctgaggt ccggagcaaa
    18061 aatcctgatc gtgccttccg atgaaaacgg gctggaccgg ttgatcgaag aaacaggttc
    18121 cggggcctcc tgttcaacgg cagccgaggg agctgcgcaa ctggaaaaat ggtattgcga
    18181 atggcttgaa ggtaaattga tccgcagagg aatagataca ggccaacagg atattttttc
    18241 ccggaattct cagagcctga ttttggttaa aagtctaaga aattatgtag aatcacatcg
    18301 aatccgttta gggagggatt aaatgtgtgt ttcacggttg gatcagattt tgtgaacatt
    18361 cccagcaggt accgtttcat tgaatttatg gcaaccattg cccgtcaatc catttatacc
    18421 tataggtttc gaattttggg ctacggctta aatatattgt tgaggccctt taagaaaaca
    18481 ttgccctctg cctggattgt tattcacaac tacgatcata acctttcaat atgtgtgaat
    18541 ccggttgagc atattggaag cactatattt tacactggtt tttatgaggc taaggagata
    18601 tgggctttaa aaaggctaat taaacccaag gaagtgattt tggatatcgg agcgaatatc
    18661 ggagcctgga ctctgatatt ggcaaagttg gtccctgaag gtcgggttta tgccttcgag
    18721 ccctcttgtt tttataagca actcaattgg aatatacaat taaatcgtga aattaagaat
    18781 atagtagccc agaatattgc cctaggtgca tatcccgcgc aagcgaccat ctcaaatcca
    18841 aacactgagc atcggtcgag cataattaat tatggaaatc taaacttaaa attagatcct
    18901 gatcaatccc tgatgggaga tcgaatccgg gttataactc ttgatgactg ggtcaggcag
    18961 gaaaaaattt ccagactgga ttggataaaa ttagacgtgg aaggtatgga gctcatggtt
    19021 ttgttgggcg ggttagattc acttataaaa ttccacccta atttgttggt tgaaattaat
    19081 gaaaatactc ttggcaacta tggctctact gctttgcaac tcagttcatt tttgatgcaa
    19141 acaggatatc gtttttttta tcgggtgggt aaaaagggag ttttgaaaaa atatgattca
    19201 atgtccactt cgatggggga atccttcaat ttgatttgtt cgttcagagc attggactga
    19261 tatggcccat atttaccata taccttcttg gtggccatcg cgaaaacatc ctgccactgc
    19321 cttgtttata tctgatttaa ttgcatcgat ccaggtgtcc cagcctcaac attaccatac
    19381 ggttggcttg catgcccata aaattaaatg gatatcgcct catcacctat ggagagacct
    19441 ccatgcatat ttcaaggatg aaactccact gcaacccaga ggctcaactt ttatagagtt
    19501 attcgaatta tcaaaaacaa ttgggcttaa tccaatcaca aggctaaact ttattcatac
    19561 actgatccga aaacatattc atttggttgg aggaatgaaa tgcagacctg atttgataca
    19621 cgtgcatgtt tcatttccat ctttacccgt tggatatgaa ttatcaaaac aattcggaat
    19681 tccgtttatt cttactgaac actcatctcg gtttcccgac ccgcgttttg aatcatatgg
    19741 gtatgaccgt gaaaccctaa ataaatattt tttgcggtca tcagcaaata tcgcagtcag
    19801 tgctgactta gctaaaaaat tcggatttta tgatattcag gatatgcaag ttattcccaa
    19861 tttagtatcc tgggctcctc ctgaaattag aatcccaggg cctcctttta gctttatact
    19921 catttcccat taccttgatc ctcgaaaaag aattgattta ctgattgatg ccgtccattt
    19981 acttcgatca tccggaaaag agtttattgt cagaatggta ggagagggcc cgatgaggaa
    20041 tctttatatc gaaaaggtaa aagcagcagg tattgctgac gtaatccaat ggtatggcca
    20101 tgtgagcgat gggcataaac aagaactatt gtctgcttca cattgcttgg ttgtatgtag
    20161 cgactatgaa actttcggag tcacggtcat tgaagcaaat gctttcggca tcccggtact
    20221 tgccacccgg tgtggagggg tagaggaaat catttcgtcc gtgaacggga gactggttga
    20281 caagggaaat tcgatccgac tggcggaagg gatggagtgg atgatgagga attactcgta
    20341 ttttgaccgg aaaagaattc aacaagattg ttatttgcgt ttttcagcaa cccggattgc
    20401 agaaaaatac ctcaatgtat atgatgagat actcctaaaa aacaaattat cttaagatta
    20461 ccaacttacc aattcctttg gtaaaaaacc gacgggcgtc ctgtccgccg gaatcctcga
    20521 agtcttcaaa cgaggaatac gatttcccct gattatcttt catgactacc cggtacagat
    20581 aagtgccgtt tgccagtttg ttgccatact catcgtgccc gtcccaggcg ccttcggtga
    20641 gatgggtgcc ttcccgcaaa ggccctaaat cgaattcggt cagttccctt accacgcggc
    20701 catttacggt caggacctgg attttaaaaa ccgctggttt ttctccggtc aaagtataag
    20761 caaagcggca ttgggtactg aatggatttg gataaggcaa tactgctgag accgtgtttt
    20821 cggtgatcac tttaaaatcg atttcataag gcacgcgacc ggcttcattc cttgaccgat
    20881 ccctggcctg gaccctcagg gtatagctgc cggtttgcac aaaaccgggc cggtaggtca
    20941 ccgaagcctt gctgccggag accgaaaggg tatattccct ggaggggatc cgaatgaact
    21001 gatcagaacc cggatatttc agactcagcg cgatttgggc ggtatcctcc ggtgagaaga
    21061 gtttattatc gctcagatcg atctggataa agggctgccg ggatacaatt tcattgttta
    21121 atatctgcct accgtcaaat aggaccgata ccgttggcgg caccaggtca tccgcgacgt
    21181 aaaaggaaat actgccggta ttgttgagaa gggtagtttc ctgcaaaccg tttttcgacc
    21241 tcgcatcgat gaccagggac tgcaatttgg acctctggtc ggtggaaaag gaattggaaa
    21301 tgctacgctt ctggccggcg cccagaggtc ctgtactcaa agtgtccctg gtagtgatat
    21361 tttggggatc agtgagtgag aaggtcaccc ggatggaatc ggtgggataa tctccaacgt
    21421 tttccaatga gaaacggatc ctggcatcct ctccgcggtc cacggtgtcc ttgtaaaact
    21481 catagtcttc atttgaattc attgctaggt cagcataacc tgtataatat acgcgccagt
    21541 attccatatt aagggaaccg gatttggatg tatccgaggc ctgccattca aatttcaagt
    21601 aaggatagga gttcggatct attcccgtta gatcaaccag actcgccgaa gttttgatca
    21661 gagtatcaaa ggcttgatta ttttttccta atactaccat tgtattttca gtatccattc
    21721 tttcatcgtt gaccgaccat tcaaattttt gccaatgaat tgaaggtcca accagctgtg
    21781 attcaaatgt gccaaaggat ctgctcaagg gtaaggaaaa agaagatctg acttgcaagc
    21841 catccgttga tactttttca tctataacct catgtccttt tcggaacgcg aagacatatg
    21901 gataggaaat caattcggtt gcaagacttc taattagcgt tgcgccatgt gactcaaagg
    21961 cctgaaatag atttttaccg agtatggtgg aatcatgagc ccaaaattca ggataatagc
    22021 tcgctcccgg ctcaatgtga tttattacta ttacatattg tccgtcctct ataccggttt
    22081 ccaaaaaccg tatcagatta cttctttctt ctatggtatt tttttccata gggaaataat
    22141 accccggggc aggaacatca aatacattta aagcgccatc acggcctccg accggatttc
    22201 tgatcattcc aatttttgga tcccaaattg tgagaatgaa ttcagaaaga aaatttctgt
    22261 tgttccgggt atgcctgatt ccgttgaata atactttaga atattccgct ggatctaaac
    22321 tttggtttat acttgatgcg acaatgctta tttgttctgg actgttttcc caacgatgat
    22381 gaagggtgtc gaaaaagata agttgacttt gggggttttg attaaattga tagtcatgag
    22441 actggttcca gcctggcccc tgccccggta ggaagatgaa tgaactttca ttccagatca
    22501 aaggccggtt ggtgcctacg gtatccgctg caacccgcca gtaataaacc gtattcggag
    22561 agagggccag gcccggccgc cattggatca tcccgccccg ctgatgcacc gtggctgagc
    22621 gttttaagga agaggaaaaa agccgggcgg tgtccatttc aaaataataa tccgactccg
    22681 gactgaacgc attggatgaa taagctttaa gcaccaggtc ggcatcactg atgatcccga
    22741 aatttcgggg atagatcggc cgcgcttcat tgctatagat cgcgaagctg atttccgaat
    22801 gacctgtgtt atcgcgcaat ggattgttgt tttcggcaaa aggggcgggc aattcctgga
    22861 aatcattatc ggggtccagg aaacatctca gcgtattgat ccctacggcg tttggaggca
    22921 ttggcaggct gaagctcacc gtttcaaacg ttcctttcag cacgacgctg aagcgatact
    22981 ccaaggtgcc gcctcccggc acaccgtgtt cgaccttgat ccctaccggt tgatctgaat
    23041 attttccgat attccagata tccaccttga aacggatgct atcctgatcc gattgaacat
    23101 tggccggttc aaacctgaag ctggaatagt caaaggtaaa atccgggctt tcataataat
    23161 tcagctgtac cgccggatcc ccgtgaaaag ttaattgttg attaagagaa taggtgggaa
    23221 aatccctcaa ccggtcaaat tttctaaggg attccctgtg aatattccca atcgatccta
    23281 cgtattgttt gttgccgagc aaattgtaaa attcattgcc ataattggag agggcgtagg
    23341 gatatccgaa ccccgaagag gcaatatacc ctatggtgcc cttcggggag agtacaaagg
    23401 attcacttag actgatgccg gtggtatgca tgttcccggt aagacagccc agggaaaaac
    23461 tcagcgggta ttttccttca ttatcaaaca gggccgggtc atccagccct atttctgttg
    23521 cactcgtagc tccatgtcct aaatatacct tcaaggcaac cccgttgttg attgcattct
    23581 tcacgatttc agacaatccg ccctgtaccg gatcggagga agttttggtt accgtggtca
    23641 cctgggcatg aaatgcgttg ccttctattt tttctttcat gctttccagg taggcgtcaa
    23701 accgttcctg gggactgccg cctgccatat gcagcactct tttcctccaa tacaggtctt
    23761 cgttggccga gggatttttc agcttgccct cgtattcctt cactttgttg agataagtga
    23821 tgacttcttc atttttcgtg gcggctatcc ttccgaaagg caataccgga actgagctgt
    23881 tgtgagaggc gaaaatgagg ttgtcagatc cgggatatcc aaaggtcggg atcaggtgga
    23941 gatgtccgta tagattgatg ttgttattta ccctcaggtc cctgtagttg atcgcccttc
    24001 cgaccagaag aaggtatttg ggagccgccc atttcctggc gatgaaattt ccgaaatttc
    24061 taaggcccat gacatgcctg gggatgccgt aggcaaactg atcatagatc tcatccacat
    24121 ccacggtcct cactcggaat ccccccccgg ctgaggattg gcgataagaa gcgtattcct
    24181 gggtactggt aaggagcgat ttgtggtgga ttaggatgta atccgaattc gggtcggaaa
    24241 gaaggggagc gagctcaatt ttagcgaggt ttccaacagg ggaagccgca gcggcctcca
    24301 ggatataaac ctggccgcta tccgcagact ggggcaactc tatataatcc tgattgtctt
    24361 cgcggatcgc ctgcgtccag attgccccgt ccggatcaat caggaccggc tggcttaaac
    24421 cggggggcac cagggtctta taagggctgc ccgctgactt caggacctca cccaccgcca
    24481 atggtttgcc gggctgtttg gggtagacca agcgaatatt ggatagtaca aaataatcca
    24541 gggggctgtc gagtccttca atcttcaggt ttatattccc ggagagggtc tcattcggga
    24601 tatccaattc gtaatttttt acctggtagc cccagaatga atcaatgacc tgaacggcat
    24661 cgttgattga aatcctgagg ttatgctgtc gggcattggt tgccagccgg atcctgagtt
    24721 taccatttat attgcctgta taaggcgagg cggcggggat cacctgaata taattccggg
    24781 attccccgtt gccaaaccct tcgcaggcat cgtaggtgga gtatgaaacg gtattttcaa
    24841 aatcgtaccg ctgatcaaaa ggcaagcggt ggaattcata aaggcgctca acccacacgt
    24901 aatccggggc ggacaacatg gtattcgatg gccttagcgt caccctcctg gtcgcgttgc
    24961 ccggagccac ccaggtcagg aaataagcca tggaatcggt ataaaggctg tattccgggt
    25021 tgagcatatc cctggccggg ttggaaaaca ggaaggcatc catatccgat ctgtttttta
    25081 tcccccagaa ctgcaggaaa tccccttccg accaggcccc ggtattggag acatacaaag
    25141 ggacttcccg gcccatcctc cacaactgaa attgtgcccc gttgatttgg cccgcgggga
    25201 ctcccgcctg aatgaggtca ttataacgga tctgataaag gctgtccttt gaaatataaa
    25261 atttgaagta ggtctgatcg tacctgatcc attcgggtcc atacagggta tcccccgcct
    25321 gaaccatctg ggctttcagc caccccggga ggcattgaaa gatgcaaatc aatcctaaga
    25381 gccgatacgt ctcagaagca ttcatccggt attaaacgaa tttaaggtag ttttggtttt
    25441 gtggtatttg cagacatcaa ttgcctttta tttatcggag cctgcctggc ccaggttttt
    25501 tttctcggtt attttatccg ccccctgcgt aaaaatacgg tgcccgtttc aaagtcctcc
    25561 ccgccgctga gcatcctgat cgtggggcgc aacgaaaggt caaatctaga gaagttattg
    25621 cccctcctgg taaaccagaa ataccctgat tttgacattc atcttgtgga tgatgcttcc
    25681 gaggatggca ccgccgacat ggtcagggat ttaatacccc ggtttccatc catccgtctg
    25741 caggtcttgc ccaggcattg cgggaaaaaa aaggccctgg agacagggtt gatgcaactg
    25801 gaccgggaac tggtccttct gaccgatgcc gattgcctgc cggccaccga ccaatggata
    25861 tccctgatgg tggaatcgct tgatccaggg tacgatgtcg ttttgggata ttctccctat
    25921 gaacagcggg agggaccatt gaatcaggtg atccgtcttg aaaccgcgta cacggcggcg
    25981 ctttattttg gttttgcaag gagaaatttg gcttatatgg gcgtgggcag gaacctgttg
    26041 taccggagaa ctgccctggt ccatgctttt aatctgaatg cccaaagagg actcaaatca
    26101 ggggatgacg accttaccat ccaggaattg tccgggcagg ggaaaataaa tttccgttat
    26161 gatccgcctt cctgggttta cagcctgcca gaaaagaact ggaaggcctg gttgaatcaa
    26221 aaaaaaaggc atgtcggcac tgcccgtcat tatcgactcc gccatcaggc tttgctgctg
    26281 atgtggtacg ggtcactcgc aggtacctgg gtgttcggcc tggtggctgc ctttcatttt
    26341 cccctgatgc tgctggtgtg gggggggtac aagggggtca gtttcaaatg gatcgtccgt
    26401 tccatgctgg agctcttcgg ggtttcgctt tcgcccagta aatggcttgg ggccgagggc
    26461 ctatatgtag gggttttact gtatcttttg cctttcagca cattcaaaaa atgggacaaa
    26521 tggtaaactt ttgggcttcc catttgttgg ctgcttagta aattggccaa ttcaggccgg
    26581 agatcatgtc cataaatgcc caatattcga tttcggacct ttcccattat acagggatca
    26641 aacctcacac gatccggatt tgggagaagc ggtacaattt gctggctccc aaaagaagct
    26701 gtaccaatat ccgcggctat gacggttgcc aactgaggaa attgctcaac atcaataccc
    26761 tgctgcattc cgggtggaaa atttcacaca tcagccagtt gtcggaagag gaaatggatg
    26821 ccgaagtgcg catggtgatg gaccggagtg aaggaagcag caagatcgat ttcctgatca
    26881 acgggcttat ggtacatatg ctgaatttcg atgagcagcg gttttcccag gtatttacac
    26941 aggccgtgga gcaatacggg ctgaaaaaca gcattgttga aatcgtctat ccttttctaa
    27001 agaagatcgg cgtcctttgg caaatcgatg atatttcccc ggcccaggaa cattttgcat
    27061 cagccatcct ccggaggaaa gtcctttcgg agattgaccg gatgccccag gcagatcatt
    27121 cgaaaaaccg gatcgtcctt tgcctgccac ccgatgaatt ccatgaattg cccctcctgc
    27181 ttgcccatta tatactgctt gaaaaaggga taaatacggt ttatctcgga agctcggtac
    27241 cgcctgaaat ggctgccgaa acggcgaaac aaaccaagtc gggcttttta tttacgctct
    27301 ttatgtccgg tgcccccgcg gaaaaaatca aaggctacct ggaaacgatc catgaaaatg
    27361 cccctgatct caaaatctat ttttccggct ccccggagac gtgtaaaaaa tcgggcctga
    27421 atgaccggat tttcagcctg gaaggcatcg aagcctttga acggtttgcc gcatcgctgc
    27481 aatgatcaaa tcctttatgc cttcgtaagg atcagggcag tatgatttct tttacgatga
    27541 ccctggtatt cacgctgtcc aggacctggg tataggcgag gtataaggtg gaatccttcg
    27601 attcaatcac cgggaagccg ctttttctta ttgaggaggt ttccgccagt cgatgatccg
    27661 agaggacctg accatccggt gagagtatag tcagtttaat cagcgcatct ccctggttta
    27721 tttcatccgt tttttccagc tgactgacag ccacccttcc cgcatcgatc caggcgatgt
    27781 ccacccttcc ggaggtattc gcggatggga tttccacggg ctggccataa gtttctcctg
    27841 cattgtttga aaagattgct ttgaccgcgg gccgtccacc ggcttccgtg taccaggcaa
    27901 tggccagttg gtttcccctg ctgcaaatgg tagggccgtt gactgggcat cctccgatct
    27961 tccagtggtc ttcaaatacg ggttggggcg tggtccactg tccggataca tatcgtacgc
    28021 tgtaaatgtc cctttcttcc tgttcattgc ggttcctgta gatgattacc ggtccccggc
    28081 ccgtcatggc cgcatccgtc tggcagcatt cacatacgcg gtcatcgatc acctcgttgt
    28141 gactgatttc accacttacg gagacgaggg cggcgcgtag attcatagca ccttcatgtc
    28201 ccatatggcc ttcatgacca ggctcgtcca cacccggatt catttggctg ccgtccagcc
    28261 aggtaaccag gattttatca aaagagaagg gaagcagggt gacgaatcca tgttcagccg
    28321 gcactgtatc atgcagtcct tcgaaaacct tgaaatgggc cccctgatca acggagaggg
    28381 ccagtttcac atcataatca taggttttgc cgccgggcct cttttggaga aaatgggcca
    28441 aaagattata ctcacttccc gggaataccg agagggccgg ataatcagcc cagttgacaa
    28501 accagtcctg gccggaggct acggtgcgtg tttccgtcca agctgaatcg gcccaggacc
    28561 tgaagcgaag actgacgcca ccatcccgct gatgttccat ccacgaaagg taaagcacac
    28621 tctgtgggga aagaaaccaa tttggggtct ctgtccgccc ctccgaaaca ggaattaact
    28681 ctctgatatg cagtgaatta tctattttac cacaggagtg gaacagtatg gtcgccaaaa
    28741 tcaaaccgaa cgggaatttc atggccttaa aattagcacc cttcaaatta aagaagaagt
    28801 ccgctgaatt aaagatattt gtaaactatc actgaatcgt ttatggccgc aattctccag
    28861 gaggcaaaaa attacgtaaa ggacctctta accagccaac tcaacggaac ctactggtat
    28921 cacaactggg aacacaccga atatgttttt gaagaagcta tggccttggg tgaatccgcc
    28981 ggcctgagtg aatccgaaaa ggagatcctg ggcctgagcg ccctgtttca tgataccggg
    29041 tttgtgaaag cctaccagga ccatgtaagc aacagtaaag taattgctaa ggagtattta
    29101 ttatcccggg gatacggcca ggaacaaact gccggggtat tggagaatat cgaagcgacg
    29161 cgcatgcccc acacggggac cggcaaactg ggtgcccttc ttcaggatgc cgatctggcc
    29221 ttgctggctt cggaagatgc cattcagaaa tcggaaaatc tccgccggga actggccgtt
    29281 ttcgagaaca aacatttttc tgacgcggaa tgggtcaaac ataacctgga tttttataaa
    29341 gcggtcgtct atcaaaccgc cgaaggcaaa cggaaatacg aagcgggaaa agaaaaaatc
    29401 agggatatcc tgaaagaaaa aaacaaaaag tccaaaaaag acgagagttc cctgatcaac
    29461 agcaaccgta ctgcggaaat gctgtttaag accgccctcc gcaatcacat caacctgact
    29521 cagatcgccg acaataagtc caacatcatg ctcagcatca atgccctgat cgtcaccttc
    29581 gccctgccgc tgatgtatga gcagatgcag tcccagcaat ggtattttct gatcccggtc
    29641 tttatcctgg gaggcacctg cctgatttcc attatttatg cggccctggc cacaaggccg
    29701 ggaacggtca agggcaagtt taatcttgcg gacatccagg cgggaaaggg cagtttgttt
    29761 ttcttcggca atttttttaa aactccgctc aaggactaca tacccgccat gaaacaggcc
    29821 cttgccaaag cggaaaattt ggacgacagc gccatcatcg atttgtattt tttaggcaaa
    29881 tccgttggcc tgaaattcga gctgctccgc aaatgttatt ctttcttttt ggtgggcatc
    29941 acgctttcca gcgtgctttt tggaattgtc tttattatcc aggccattgg aaatcagggt
    30001 taaggattaa ggtttaaata aaaaaccctg cagcctttga aagccgcagg gtcccgcttg
    30061 ttgatgatca aagggatgta ctggtcagaa tttgaaaatg gcggacatgc ccagggtggc
    30121 ctggctgtcg gtcgtaccgt ctgcatcgag atacaggtcg gtgtgtccat tgatgctgtt
    30181 tttacccttt gaactgtcga tgcgcagttc cggtttcagc aggaggtcgc cgccggagag
    30241 gctgattacc gaggtcaggg tgaaggagct gttttgggct cccaggtacc tcaccgaata
    30301 gtagtcgctg aattgttcga agcgggctcc gagggaaaac atttcggaaa gggcaacgct
    30361 ggcataccct gccacacctc cccagtccgg attgtccttt tcttccttgg tgatcttttt
    30421 aaattgggtt tttacgctgt tttccttggc tttgtaaata ccataggccg catttacccc
    30481 aaaggtgaat tttcccgtat tgtaacccgc ggtgagatcg taaatgctgg tgttgaatcc
    30541 gttggcatcg ggcaattcat caaagaccgg attgagggaa ccggcccggt cgcccttccc
    30601 ggaaaggtaa ttcaggtaaa tcgataagcc gtccatgggg aaaagcgata cctggaagac
    30661 gggggattta aactcattgt tgtcatcaaa attgtcccag gtattcacga ggccggccat
    30721 gatcccgatt ttatcggaaa tggtatagtt ggccttcagg ccggtatgaa agaagggacc
    30781 gttgttgaac aggttggaca gggaataatg gaaattgatg gaagattcga tcacttcata
    30841 accgatatgg gtcccaaact ggccaatggt gaaagacaat ttgtccgtgg ctttccaggt
    30901 gccgtaaagc tgtttgatcc cgaagacccc ggcgccgagg gataccggga aggaaacccc
    30961 ctggaagctg gccagggttg ccaggctggt attgggtccg gccaggaggt cggctaccag
    31021 ttccacctta tcgtgggtat aggacaattt gccctggatc aaacccaggg agaattcgtt
    31081 gctgttgatg tcaaaggcgc gggacccggc agccggaccc cagagatttg ccccgacccc
    31141 ggcattgtta aaattgaagt tgtagtacat gtcggcatag ccgctcactt taaatgcctt
    31201 cggcgggatg gaatcctggg ctttggaggt atacatcacg gtcaggcaaa gcagcaagta
    31261 aatggttttg tttaaaaatt tcatagtctt gagtttgtta ctggctcaaa tgtaaatggg
    31321 ggtggaccga ggaaaaattc aaaaaccagt aattcttgta attttcaatt tttataaaaa
    31381 cagcattaaa atttaagtga tatgctatta aaaaatggaa aatcagtaaa aacaggttta
    31441 aaatgggctt tatttttgta aaatacagga aaaatagcca atactaagtt tatgggattg
    31501 tgccgttaaa gccgagtccg ccaatccttt tttccttcgt ttccagggta atcaacgctg
    31561 aaaacgggtc ttatgggttt gcattccgga gccgggaatg aataaaaacc gtattttcaa
    31621 cgcaattaaa ttgaatgatc atgaataagt cttccaggcg cagattcctt cgccagtttg
    31681 gcaccggttc cctggcttta tccggcggtt ggttcagtca ggcggtcgca gaagaatatt
    31741 atgagcggca ggtcctgccc tataataaaa tctattcggc caatgaccgg ataggaatag
    31801 gcgttattgg ctgtgggatc cagggcaatt ttgaccttcg ggcggccctc caggtccccg
    31861 gagcccaatt ggcaggggcc tgtgaccttt ataccggcag gctgaccagg atgaaagaag
    31921 tctataggca ggatctttac accacccggg actacaggga attgcttcag cgaaaggagg
    31981 tggatgcggt catcgtggct accagtgacc actggcacag ccgaatagcc atagatgcgc
    32041 tcaaagccgg caaacatgtg tattgtgaaa aacccatggt acatctggtc aatcagggtc
    32101 atgccgtcat caatgcccaa aaggaatccg gcaaggtact ccaggtggga agccagggga
    32161 tcagcggcat tgactttgct aaagccaggg aattgtaccg gagcgggaca atcgggaaac
    32221 tgaattgcat cgaagcttcc aacgaccgtc aaaacgccat aggggcctgg caatacagca
    32281 tcccctcgga ccagtcgccg gaaacggtgg actgggacaa atacgtcgag gtcctcagta
    32341 aaaaacctgc atacgatgcc aaaaaattct tttggtggcg gaattaccgg gaattcggga
    32401 cgggcatggc cggcgatctt tatgtacatc tcatcacagg aattcattat gtgaccggat
    32461 ctctggggcc cagccggatc ctgtcatccg gggaattgtc ttattggaaa gacggaagga
    32521 atgttcccga tgtcctgctc accatcctcg attatgacga aacaccccaa catcccgcct
    32581 tccaggtcat gctgcgatgc aattttgtca gtggagcggg aggaagcggc ggcaccaaat
    32641 ttattggcag cgaaggcgtt cttaccaaga ccggaaatgg ggtgaaggtc cagcacagtc
    32701 tgatgtccaa ggcacccggc atcggcggtt acgattccct gtttacttac ccccaggccc
    32761 ttcaggacaa aatgatggcc gaatacaatg ccaaatggtc ggaggaagat aaaaaaaggc
    32821 ctacccggcc caacattgag tattcggcgc cggagggggc caatgcccat gtagaccatt
    32881 tcagcaattt ttttgaaagt atccggaaca acaaacccaa tatcgaagat gccacctttg
    32941 ggttccgggc agccgggccc tgcctcgccg caaacgaaag ttattttcag aagaaaatca
    33001 taaaatggga tcccacggcc atgaaactga aaaaataatc gtttcagttc catgattatg
    33061 cccccgggcc gaaaagccct ccctgcggtc cttttgggat ttggcctttg ctttgcctcc
    33121 tgcagaaaag aggacgatgc ctttcttttc cggatggaat acgaccagat cattgccctg
    33181 cccccgggac taaataccgt ggagacctat acgtttgtga tccgggacct gcccacccat
    33241 tttcaggcct tgctttccac acacggggtt gctgaggagt cggtgcggtc ggtcaatccc
    33301 ggagcgatcc ggttgtccga tgagttgaac caactcgatt tcaaccggtt tgaaaaagta
    33361 tccctgctgg caagcaccgg ggattttaaa aatgaaaaag aaatcggtta ccaggagacg
    33421 attccgctga attccagcaa ccaattgcag ttgttcccca ccctggtcga tgcccgggac
    33481 attttttcac aaaacacctt caacctccgt ttgaaaatca aacttagaaa ctttctttct
    33541 tccacttcca atatgcggct caggatttcc atgaatgcaa gggaaagtca gtagtcaaga
    33601 gtcaatagtc aagagtcaat agtcaatagt ctagagtcag taattgggaa tcagtgggca
    33661 ggattggaat tttacccgga tcgatttaag ccggtttacg gcccagccac catccgatta
    33721 ccgcaccggc cgcagcggca aagacgacgt tcagggcgat atccagaagc atgaaattca
    33781 ttttcagcag gttggtcgtg gcgtacatgc cgaaatcaaa actcaggatc atgagcaaac
    33841 ccaggagggc gccggcgctt gctccgccgc gtgcggtgcg gatattggcc cagcggtcaa
    33901 aaacgtaggc aaacaggagg ctgcttgcga aagaaccgag gaagataaag acgtaactgg
    33961 aagcccccat ttctgcctcc gttttcatgg caccgggaat catgttggta gacatgtaat
    34021 ccataagtaa aagtccgtag caaataaagc cgaggatgaa actggtcaat gcgcccaaca
    34081 gggcggcaac aataacttta ctgttcataa aaaggtattt ttaaggtttt tgattttaaa
    34141 tggaatggga atgaagcagt caataatctt taatccgttt gaaaaggtgg gaaggccgga
    34201 agttctgcat tcagaaagtc ggtagcccgg aaatctaaat aaaagaaaag ctccatgacc
    34261 attcgatgtc tgaatgcccg ggattttatc cagcaggatt acggcccagc caccatccga
    34321 tggcagcagc cccggcagcg gtgaccaggg tacttgcgag tacatcgaca atgaccttgc
    34381 caagcccgct gaacatatgg gtggtggcaa gcaggcccag gtccacactc agagccatca
    34441 ataccccgat cagggctcct gcccttaagc cgccctggag cgtacggata ttcgcccagc
    34501 tgtcaaagat gtatgcgatc agcaggctgg aaacaaaaga agcgaggaat atatatacat
    34561 aggaggcgcc agtcatttct tcagccgttt tcatcaggcc cggactcatg ccggcttcca
    34621 tgtagtttcc aagaaggagt ccgaagacga taaatccagc gataaaactg gccacggccc
    34681 caatcagggc cgccagcagg actttactgt tcatatgtat tttatttaag gtttagggtt
    34741 ttgaatataa taaaataaat tcatattagt taaaataatt tatatactat cagctttcag
    34801 ctttcagaaa tccgctttca gctcatggct gatagctgat tgcttatctc tatatttgcc
    34861 tccgtttttt taatttataa atgaaagtca ccgagtattt tgagaaggcc cagggccaaa
    34921 ctttgatttc ttttgaaatt ttacctcctt taaaaggtgg cagcattgat tccatattca
    34981 aaatcctgga tcccctgatg gaattccgtc ctccctttgt ggatgttacc tatcaccggg
    35041 aagactttat ttaccggaaa aacgatgacg gtttttttga aaagatagcg atccggaaaa
    35101 ggccgggtac ggtggtgatc gcccaggcca ttcaaaaccg gtacggcgtg gatgcagtgc
    35161 cgcacctgat ctgcggggga tttacccggc aggagacgga agatgccctg atcgacatgg
    35221 cttatgctaa agtaaacaat gtgcttgccc tgagggggga tgcccggaaa tttgaaggcc
    35281 gttttattcc tgaacccgaa ggccatcatt acgccattga tctgatcagg cagatcatgg
    35341 atatgaacca gggcaaatac ctcgacgcag acatcgtgga cggtgcaaaa tctaattttt
    35401 gtatcgggat agccggatat ccggaaaaac attttgaagc gccgaacatg gaatcggaca
    35461 tccactacat ccgggaaaaa ataaaagccg gcggggaata tatcgtgacc cagatgtttt
    35521 tcgataataa aaaatatttt gagtttgttg acttgtgcag gcagaatggg atcaccgtcc
    35581 ccattgtgcc gggcttaaag cccctgaccc ggaaaaacca gattcaaagc ctgcctgcgt
    35641 ctttctacat caacctccct gaagaccttg tgaaagccgc ggaaggcgcc aagaatacgg
    35701 aagccgtcaa acaggttgga atcgaatggg ccgttgccca atgccaggaa ttaaaaaaag
    35761 caaaggtgcc ctccctgcat ttttatacca tgggcgacag cgaaaccacc aggaaaattg
    35821 cggaaaaggt attctaagcc caacttgcca actgattact tgccaactaa aacgaccttc
    35881 ccggtgtata atctcctgcc ggcctcaatc ttataaaaat acattccgga agcccatcct
    35941 gaggtgacca gttcgatccg gtcaacagaa ataaacgact gggtgaacat tgagcgaccg
    36001 tgcaaatcgt ggatgaggag tttaccggat agggtaccgc cggcaaagtc aaacaccagg
    36061 ccccggctaa atggattggg gaatgcccgg atatttacca cggtggaatt cacaattccg
    36121 gtgctgagca tttcttttgc cgttttccag gcccggaacg cattcagtct tccgtaacca
    36181 tagacaatat tcgggatgac catgccactg tccgctccac aggtaaaacg aagggtcttt
    36241 tgctctgcgc ttttttccag gatgtcttcc agcagttcca ccttgccggc gaggcttggg
    36301 aacgctgaaa tcaggagggc taccgtgccg cttacatgcg gagaggccat gctggtgccg
    36361 ttgtaagcgg cataagcccc attgcggatg gttgaccgga cggccacccc gggcgcggtt
    36421 atgtttggtt ttattctgaa actggagtcg gtgagtaccg gcccccggct gctgaaagaa
    36481 gcaatggtat cgttgaaagc cgtggcgcct acactgaagg agggttcata aatagcaggt
    36541 ggattctgga ttgaactgca acccagggag ccttcgttcc ccgtagaaac ggtaacaaaa
    36601 attcctgccg ctttcagggt cctgatcacc tgttccatag tctgaaaatt ggaaggattg
    36661 catccttcct ctttgggaca gccccagctg ttgttgatga catggggtgc ctttgaagga
    36721 tccggatttt gtccgttgag gtcggtgggt gccagaaacc attcaaaaca ttctatatag
    36781 gtagtcaggg taccccaacc cctttccatg cagcggcaac cgatccattt tgcttcaggg
    36841 gccactccga tctgaaaatc cgtagccgcc atggtgccga cggtatgggt gccgtgattg
    36901 tggtcgtcac aaggggtctg gacatcgagg ccacaaggat tgttcatggg gttatcagtg
    36961 ctgtccttat tcaggggatg aaggctgtga atggcatcgt gccagttgta atggtgatca
    37021 agcgtattaa aatccaaggt cccccgatag ctcccccgga tagccgggtg gagttcgacg
    37081 ccggtatctt cacttccgag tacggtcccc ttcccggtga tacccatgga ccataaggta
    37141 tccgcctgga tccgttcgat tccccattcc gcggcaagat caggacttcg ggactccggg
    37201 atacggatat ccgataccgt ttgcacgggt ttgaccaggg catacgggga gttgaattca
    37261 atccgggcca cctcctccag cccggacaga tactcgatca atgggtaatc ggctgccgga
    37321 atatggatgc cattgataat aaaaaaagcc cggtggttta tctgattccg cgtcaggtga
    37381 cggcgtactg atctttgact gacccgggct tctgcccgga gtttttcaaa aataaaccgg
    37441 cccttatctt ccttggtttc catcttgccg gctaagggaa gccatccgtc agtccccgat
    37501 ttgaataaga ccaggacatc tgcagtcggg tgcttgtgga actgttccag gaccaaaggg
    37561 tcaatatatg caggggcagg cggattaacg aaggccgtca gcaggatcag gattacattc
    37621 accatagaat aaataaaagg tcaaagatat gggaatacct cgccggatta aagcaagtca
    37681 accgtccctg aaccgttatt ccgggcgtct gtcaaagcat tgaacccccg aattttatga
    37741 gcggaatccg gagaatttag ctaactttcc cacccttcgt ttaatcacca gctgaaccat
    37801 ggatatcgcg cattatactt tatcggatac cgaacgacag tcattcgaaa aattgcagga
    37861 agaactcata ccccagttcc agttgcagtt tcctgacccg atggccccta aaacggtgat
    37921 catagtgccc agcctgagcc tggattcaga gatcctgtca aaaatttcgg gtcatattca
    37981 ttacgaagaa cgtttgcttt gcctgttgct gttgctccgc atgcctcgca cccaggtcat
    38041 ctacctgagc agcatgccca ttgatgaggt cattgtggat tattacctcc acctgatcca
    38101 gggcatcacc catatgcatg cgcgcaaaag attgagcctg ttggcctgtt acgatgcctc
    38161 caatatcccc ttgactgaaa aaatcctgga aaggcctcga ttgatggagc gcatccgtag
    38221 cctggttcct cccgggcatg tagcccatat cgccgcattt aatgtcacgc ccctggagga
    38281 gcagctcgcc ttgaaactgg gatttcccct ctacggctgc ccttcttccc tgtcttattg
    38341 gggttccaag agcggcagca gggaattgtt ccgcaatgcg ggcattccca tgcccccggg
    38401 ttatgaaaat ctcaaaagca ccgaggatgt cttgcaggcc ctgatgaatc taaaggcaga
    38461 acaaccgcat cttacaaagg cggtattgaa acttaatgaa gggttttcag gcgacggcaa
    38521 tgcgatctat actttcggca agctgcctaa aatcaccgaa gacctcttgc gcaacaatct
    38581 caaaatggtg gcctccgaca tggtctttga tcagtttatg gagaaattca accagatggg
    38641 cggcatcgtg gaagcttttt tgccgggggt ccaggcttcg ccctccgttc aatgcctgat
    38701 caatccgctg caggagattg atgtcgtttc tactcacgat cagatcctgg gcggcgaatc
    38761 gggccaggtt tatcttggcg cccgctttcc tgccgatgat gcttaccgga gggaaatcgg
    38821 ggaattgggc tataaattat ccaaacacat gtccacgctt ggtgtcctgg gccggtttgg
    38881 ggtggatttt gtatcggtga aggacggcga agagtggaaa cattatgcca ttgaaatcaa
    38941 cctgcgcaaa ggcgggacct cacatcctta cctgatgctg caattgctca cgggcggcag
    39001 atacgattat aaggaaggga aatacattat gcccaacggc cagacccgga gttacatcgc
    39061 aaccgatgcc ctggtctatg acaattttaa gaaactgact ccgccggacc tgatcgatgt
    39121 ggccatttgc aacaatcttc attttgatgg gaccaccgaa gaaggagtgg gttttcattt
    39181 aatcggagca atgtcccaac atggaaaact gggagtggtt tccataggca ccacggcaga
    39241 aagggccgaa gccttataca acaggaccat ttccgtattg ctgggcgaaa gccgctaacg
    39301 taccagacct ctttaaccat ttacataaat caaaccaaca tgttttttaa aaaagcttta
    39361 atcctttttc aggtcttcgg catttccctc caggcccaga gcccactgcc ccagataaaa
    39421 aaatggctgg atgattcgtc ttacctggaa acccggatcg aggacatgaa gtcaaaaaca
    39481 tatagggtat tcgtaagttc aggcaaggcg gtaccctatg ccgaacccgc tgcatccgtc
    39541 agcccggaat tgccggatag tcttcgcatg tattcctggg gacaatccaa ggaaggcaaa
    39601 ttcgtattaa aaaggggcac ggatcttttt ctttatgacg gagccctgcg caccctgacg
    39661 gcggatgccg aagaagaaaa agtcccccag ttttcacccg atgggaaaaa actggcctat
    39721 gtaaagggag gcaatctatg ggtggcagac ctccagaaca acctgcatcg ccaattgacc
    39781 agcgacggaa atgaatcggt ttacaacggg tatgcttcct gggtttattt tgaagagatc
    39841 ctgggcaggt cctccaggta tcgcgccttt tggtggagcc ccgattcccg catgcttgct
    39901 tttttgcata ccgacgacac tccggtgcct aaattcccgg tttaccggtc ggaaggtaat
    39961 cggggtcatc tcgaaatgga gcattatcca aaatccggtg acccgaatcc ccgggtagag
    40021 atgcggcttg ccgagctgca aacaggggat atccagcggg tggaagagga ctcgatggct
    40081 gatcaatata cggcctggcc cttctggacc cccgacagta aaaaactgat tttccaggag
    40141 cttaacaggg atcagaccca tctccgtttc tatgcttatg atcccgctgc caggacaaaa
    40201 actttcctct tcgatgaaaa atatcctacc tgggttgaat ttcacgaaca gatttggttt
    40261 ttgaaggaca acagtttcct gctgatccat cccctggacg gctggtggaa tatctacaag
    40321 taccaatttc cggagaaaaa actcagccag cttacccgtt ttgactggag agtgaatgat
    40381 atcgtccgcg tggatgaaac ccaaaaccgg atcctcttta ccggcaccgg ggagaataaa
    40441 atgaacaacc atctttaccg tatcaacctg gatgggtctg gccttcgcca gctgacgcat
    40501 ggggatggaa gccaccggac agatctttct cccggtggaa gcttttttat tgattcctgg
    40561 agcaacttat cgacaccccg gtccatgtat attgcaaata cggaaggaaa gaaaatccgc
    40621 gaactgggaa tagccaacga tggcaataaa attgcaggga tcaaaaccga atattttacc
    40681 gtgccttccg gggatggtta cgatctgccg gtggtctgga acctaccccc tgattttgaa
    40741 agcggaaaag cagcgggtaa agtctttccg gtggtcatca gcatttatgg cggcccggac
    40801 gcgggcactc ttcgtaatgc ttaccgggac ctgtccaacg acaaactgat ccaacagggg
    40861 gtcatcctcc tgtccacgga ccacagggcc tcgggcaagt tcggcaagaa ggggctggat
    40921 tttatgcacc ggaacctcgg caaatgggaa attcacgact atgccaaggt tgcggaatgg
    40981 ctgaaacaac aacctttcat cgacagcagt cacatcgcca tcgaaggcgg aagttacgga
    41041 ggatacatca ccgccctcgc tttgaccaaa gcgccccatg cctttaccca tggaatttcc
    41101 tttgctccag tcatcgactg ggcgctatat gataacgtgt ataccgaacg gtatatggat
    41161 acccctaaag acaatccgga aggttataaa cagggatcgg tgctcaacta taccgaccag
    41221 cttaagggca agctgtttat cgtccacggc agcgcggacg ataacgtaca tatgcagaat
    41281 acgtttcagt tgatcgaaaa attgcagctc accggcaaac agtttgaaat gatgatttat
    41341 cccaatgtgc gccatggatg gggagggcca agggccagtc acctgaacaa cctggaactg
    41401 gattggtggt cgaagcaaaa ttttgtggcc aaaaaaccgg agccgaaaag ttaaggacgt
    41461 ttaacgattg gaaatggttg tatgaaatca agttttaacc ggccaaccgt tttccctaag
    41521 acctggttat attttctcat cggtctctgg gccagcctga gtcattgcgg ccaaatcccc
    41581 gaactgcctt atgaagaccg gtcccatgcc agccgggtat ttggaaagga aaaacaatac
    41641 cggctttatc tccctaaggg ttatgaccga tccaccgtaa ggtatccggt cgtctatttt
    41701 ttccatggct ggggcggccg gcattttacc gaccccagcg ccaaactgga atatgaaaaa
    41761 ctcggggaat tggtgaataa ataccaggcc atcctggtga tgtgggacgg gaatattgat
    41821 gaaaaagaac caaggcctta taatatcggc aaccatgaaa acatcacttc caatattcag
    41881 ttgtcggagt attttccgga attggtggac tatattgact ctacctacag gacgctcgcg
    41941 gaccgggacc accgcggcat cattggattc agcatgggtg gttttatgtc ctggttcctg
    42001 gccggtaaat atccccatct ggtcacggct gcggtgaata tgaccggcag cccggagttt
    42061 tttgtaggct atccggagaa tcataccctg tacccgctgc gatacacttt taaaaacctg
    42121 aaaggagtgc ggttgcgttt tcacaacagt acggccgatg agctctgcaa actcaacctg
    42181 gaagtacacc agggcgccct gtgggaaggg ggcctggatt atgaatactg gaccttcgaa
    42241 ggcggtcata aggtggatga tccgggcaag acggaagtct ttgaaaaggc ggtgtctttt
    42301 gtggccaccg ctttcaataa cccattgtca aagccggacc aatggggaca ttatgacctt
    42361 tatccccaat tttccgtctg ggattaccag gtaaaatcgg ataaaaatga acccggattc
    42421 atcttcctta gaagggtttc cggcaaagga tttggccttt atacgcaccg ttggttgcct
    42481 gatggtccgc ctgtgaatgc ctgccggata gaagtaaaaa ccgcgccggt ctataaaccc
    42541 gggactgcct atataatcag ccgctattct aaagcggaca agaaggtaag ccaggatacg
    42601 gtcaccagtg acccggaggg ttgtttgacg atccgggcgg atcaaaacgg atatgagttc
    42661 ggggtagccc tgcaggaggt ctcgccggat ctggtggtcg gcggttatca aatggaaaac
    42721 cagtttcgtt atctccggac tgaccgggca aacaaattaa ccctgacgct gtttagccgg
    42781 ggtccggaaa tcacaggtaa tcataaagcc aggatagaag tccgttccaa ggatacctcg
    42841 gtcaaagtgc gaatggaaaa tccggtaatc gacctgtttt cgggcgaacg tgtttttcag
    42901 agcccggttt ttaccttgga atgcaaaaag caggctcccc ccaatgccca accttcccag
    42961 gtcaaaataa acctcattct gcacctggac agcatgaaat ttgaggatga actgattttg
    43021 ccggtgctgt tcgaggcccc tgaggcaacc gacctcctga tcgatgacgg gcgacgatgg
    43081 aatgactccc tgcccatact tggaataggc aatggagacg gcctcgccgc acccggtgaa
    43141 gaaatcatga tctatgtgaa tggacaccgg acacgattct atacggatga ttcctttgtg
    43201 cttcaggaca aagagcggct ggtagatgaa gtggttcctg cgaaatggcc cgatggtttt
    43261 accctgaatt ccgtgatccg catggatcct tcttgtcctg acgggcatca ggtagaaggc
    43321 ctggccagct atgagaccaa agggtttatg ccgatcgaca ggaaactaac ctggcacagg
    43381 gtgaagttcc gggtgaaaaa gtaagtctaa gggaaccggg gaggactatg ggttaaagct
    43441 caaccgattc tacataaata cccagcaata tatgccagag caaataatgc ccgaggcgaa
    43501 taaatagggt tgccgggaat cccgcttttt tataccaggc cgcctggaaa agattcattg
    43561 tgaatccggt ggcaaaagaa taaaaaatca gcaaagcgct gccacccgaa tattgttcca
    43621 ggggttcccg gaccgaagtg agtatcgctg ccgtccagaa aaacaggtgt gcgtatttcc
    43681 cctttttata cccatggcca atcgcgcaaa tcagggtcag cgggatcagg cggtaaaaaa
    43741 cttccacttc cagcgctccg gaaaaataca ggaagagaga ataaggaaaa ggctgcagaa
    43801 aagggggaag ctcggtatac ggttcagggt gcaacaggac tttgaacacc agtacatcca
    43861 ggatgccgaa gaccatccca atcaaaaccg gcaataaaat gcgggtcctg tggctgatct
    43921 ggctgtccca aaaatcgggt aaacctgctt tggtttggaa aaaaagaaag ggcaccccaa
    43981 ttaaaagcag caagagggtt tgcacatccc ataccctcaa atggttaaac gaaggcagta
    44041 tggatttccc gtagactaag cccgcagcgg cgacggacag aaaaaaacca taggtaatca
    44101 gattggattt aattccccgg gtcatgccgg gaaatatagt ttaatccttg caaacccaat
    44161 aatcccgcta ccgatttcag ttcaggtttt cgattacctt catcagtttt tgcctgacgt
    44221 ccgcagccac atcacccaat gcctcgttct ccacggacat catggaagct accggatcca
    44281 ccgcggagac ttcgacctcc cctccgggat tttcttccac gatcacgttg catggcagga
    44341 agaccccgat tttgtcttcg ctgcttattg atttaaaggc aaattgtgga ttgcaggcac
    44401 caaggatttt gtattttttg aaatcaacat ccagtttttt cttaaaagtg gactggacat
    44461 ctatttccgt aagaatgcca aatccctctt tctttaattc ctccgtcacc ctttcaatgg
    44521 cccggtcgaa ggggattccc ttcaggacct agttaaaata atatttcatt tattttcaat
    44581 ttggttatta atgcaaaggt actggtaagc aggcccgcaa agcacctccc gggaaaggaa
    44641 gattgaaaaa atgtggcttt ttaggtattt ttagatcctg ccttattgca aggctgaaac
    44701 aaacccctat ggatgatcgt cggatttttt taggtatcaa attcctgcta tttccttttt
    44761 tattcagctg gaccggatgt gaccaaccgc ccgggacagg agatacgact acgaaaataa
    44821 cccctgctcc ggaacaaata atctggcatg tgaaagcctt cctgcccgat gggaaaactc
    44881 tggatattaa agcactggat caggaaggcc ggatatatga tgttaaagcc ctccagcaag
    44941 gagacaacct ccatatgatg gatattaaag ccctgttcaa cgggacaacc ttgccggtta
    45001 aaatgttagt gagtgatgaa aaatacgctc cggtgaaagc catcggggag gacggcacct
    45061 tgtatgacat caaagcactg acgggggatg gtcaacggct ggatgtgaaa gggattaacc
    45121 tttctggaaa tgtgatcagc ctcaaagcca tcaaccaggc cggtgaatat tacggagtca
    45181 aagccttgtc caggaatggt caactttatg atgtgaaagg aataaaactt tcatctgacc
    45241 tcgtggaagg aagagtgaac ggggttgatt tttatgcgca catcaaagcc ctaccccaga
    45301 ttggccagga gggccaggac tcgatctggc atataaaagc cattcatccg gaaggaagga
    45361 tcctggatgt caaagccctg gacaaggagg gccgtatcca tgatgtcaag gcggtgatca
    45421 aagacgggga tctccacctg atcgatgtca aggcattcat tggaggcaaa caacaaccgg
    45481 taaaaatgct ggtgagcgat gaatcgaaca ttcctgtaaa agccattgga gaagacggaa
    45541 cgatttatga tatcaaggcc cttaccgccg aaggagacaa attggatgtc aaaggaatcc
    45601 gtactcccgg gaacattatc cagattaagg ctctcggcaa ggaaggagcc ttgtacggca
    45661 taaaagccat ttcaccaacc ggcaatcttc atgatgtcaa gggagtcaaa gtttccggcg
    45721 aagaggtgga actaaaactc aacggagtag aagtttatgc ccacgtcaaa gcgttgcccg
    45781 tggtccccga ccggtagagg aaatcaattg ccttttctct atggcggagt cattttttat
    45841 tatgttccac ccaaatcagt tcttctgttt taaaccccag gtcctttgcg attttcaggt
    45901 agttttgttt tacttcatcc gggatcgtga tctcccgtga aaggatccag aggtaagaaa
    45961 ggtttttgcc tgcaacaagg gcatatttat attccggatc taaagcaatg acattataac
    46021 cggaataaaa tggtccgaaa aaggagactt tcaattgggc aagttcctta tcgcccctga
    46081 atttggcctt accggtagct tctttccatt cctgggtttt ataattgaat ccgcggttta
    46141 ccacttttat gcttccgtca ggattggcgg tataggttgc agaagtattg ttgaggttac
    46201 gttcaaacct gaaatccagg cgggcgattt cataccactt gcccagataa cggtcgacct
    46261 caaacggctt tatggccgtt ccccccttgg gtatggacct gcagccggac catgaaataa
    46321 aaaaaatcat cacaatcaga atattataaa accctctgaa catttcctta aagatgtctc
    46381 ttttacaatc cggatgacca tgattctaat caggggcatc catgatttaa acttcaaaag
    46441 gcaaggtatt gttcttttat attggaaaag atgaaaagga cgagattgag ttttatgctg
    46501 cgaggcaaac catccggtga tcaccgcagg cagtggatga ctttcattct ggtcttttca
    46561 tgttttttta tcacaggcca ggtctccgat cctgaggccg tgaaatcaat gatcagccgg
    46621 tttaagcagg atgcccgcgg gccctaccgg gatatccgct ggttctgcaa agacggttcc
    46681 acaagggctg ctcgcgaccc ctgcacggac caaacgggta accaaagagc acggtataaa
    46741 gaagaagtag acctgttggc ggccagggac catatcttcc tggggcaaat cctcgccact
    46801 actccctata cggatatctg ggacgaatcc tccgcccatt cccgaatgat ccaataccag
    46861 cttgaacaat atctccgttc catggacaat ggctggatcc atcgtcgcgc gcaattttac
    46921 cggggagcgg tccaaattga agacgagcac cgctggggag cgggctttta tcaaagcttg
    46981 ctggaagata ccttcagcat ccattcccgt tattttctcc tgcggcaaag tgccagggac
    47041 attcctcatt ccggggatga taatacagcg cagcggatac gctctttgtg caaagaaatt
    47101 tcagacagcc tgccttcttt tatggacctc cgggtgaaga tccacggcgc ccccgatgcc
    47161 ggagatatac aaaaggtcaa ccatttccgg caggggatcg ggaaggaaat gactgccgca
    47221 ttgaacgaga aatttgacct cttgctccgc gaattaacca ttatgtacca acccttcatg
    47281 gtcagcgatc ttgaggatta ccggagggtc ctgccggaaa cctctcccgc aaagggattg
    47341 gtgaccgtct ttatgaatgg atatcctctt ttggccaagc ctgaacaacg ctgccggctc
    47401 atcagccgga cagctatgat gttgcgccac cagttggcct ctccaatgca ggggatttcc
    47461 aggttggccc tgatcgatgt atccaataaa atggaaaacc tgatcattat tgaagcctcc
    47521 aggtggaagg cgggatcggt gagagaattg atatcccagg tttattgcct ttccgaagcg
    47581 gtggcagcct ttggctacct ggagttatgg gaatgggagc aggtcagcag cagtttggaa
    47641 cttcctgccg gagattcgat ttctttgaat caattaaatg cactccatga aggtagccgg
    47701 cgtgtcttgg aatgggcaac gggcctgttc agggcgcatt atcagcctgt ggtcaaccga
    47761 taccgggatt ttgaaccgct tgctgccgga tttttggacg accgtgtacg ttcctcggtc
    47821 ctgttgcatt tggggcgggc ggtcagccaa ttgggtgaag cctgggcccg ggaagccgga
    47881 ttttcaaatt ctgtattggg gatagcaggc caatcttcaa tacggggact caacccgggt
    47941 tttgctttgg gtgaattggt cgtggtcact ggaaccccgg aaaaggaaga tctttccagc
    48001 agcaagattt atgtcttcca tcatccgccc ggcaatttaa agccggtggc cggcattttg
    48061 acggtcagcg aaggcaatat ggtttctcat gtgcaattgc tggcccgaaa cctgggaatt
    48121 cccaatgcgg tcatctcaca agaaaatatg gaatccctca aagccttcca tgggaccccg
    48181 gttttttacg ccgtatccca gaaaggcacc gtgatcataa agccggcggc cgccatggaa
    48241 ccgcgagaaa aggaactttt tgaacagaaa aaaaggaagg cagaaaagat atccgtaccc
    48301 atggaccgta tagtactgga ccaagcccaa atccttaacc tgcggcaggt cagcgcctct
    48361 gaatcgggca aaacctgtgg gcctaaggct gcccatcttg gccagttaaa acaaatattc
    48421 cccgggcagg tcgtggaagg acttgttctc ccctttgccg tattcaagca acacatggat
    48481 caaaggattc cgtcttacga aaccagttac tgggacctga tgaaaggaat ctttaccacc
    48541 gccgaacgga tgcgggcaag aggagacccg gaagcggaga ccgaggcttt catccttaag
    48601 ggcttggatt cactgcgaag gctgatccga aagatgcctt tactttataa cttcagaaaa
    48661 gagctggaaa aacaatttca gaccgttttc agccagcctt tgggaaaaat tccggtcttt
    48721 gtgcgcagcg ataccaatat ggaggacctc agggatttca gcggggctgg gctcaactta
    48781 accgttttta atgtattgga ctccgaaaag atctaccagg gtatccggga tgtctgggct
    48841 tccccttata cggaacgcag tttcagatgg agacaacact acctggaaaa cccggaaaat
    48901 gtcttccctt ccattctgat cattccaagt gtggatcccg attgttcggg ggtgttgatt
    48961 acaaaaggcc tcagctccgg aagcgagggg gatctcacgg tagccttcaa ccgcggagta
    49021 gctggcgcgg tcgaaggcca ggtggctgaa acctggttgc ttgatgcaga aggtaaggag
    49081 cacctggtct cccctgccag agagcttttt tacctgacca ttccggcgac gggcggcagt
    49141 ttgaaagtcc ggaccggatt agaaaaacgg atcctcactc ctgagcgtct tggggcactg
    49201 agaaaaattt cgcaacacct tattgaggaa ctgtcggctt cgccggaggt gaataaccgg
    49261 ggtccattgg atgtggagtt ggggtttttg ggtgataaga tctggctgtt ccaggtcagg
    49321 ccatttgtcg aaaacaaact ggccgccgcc acggaatacc tgaggcaaat caccccggca
    49381 ttcgatggcg atcgttcggt atccctgaat tcaaaattag atgaatgatg gtcaggtgct
    49441 ccgtattctg ttttgtcctc atctgcatat tcattttgga agcggcctcc tacccgatcg
    49501 acggatacag tcacacccat atccggaggc tgaaatatct tcaaatggta gtggatgggg
    49561 aactcaaggg aaccaagcct attccgggcg ccatgaaatc ttattccgac atccgccttc
    49621 atctgaccgg tgaaagggga gattccctgg ctgtcatccc gtcgccggat gcggacttgc
    49681 aagaaaaaat ccatggcttg tttccagtcc tcggtgaaaa ctacagcatt gcccttctcg
    49741 atatcacccc gggtcgcccc ctgagatatg ccggtcgaaa agaacatgaa ggttttcaac
    49801 ccggcagtgt ggggaaactc gccgttttgt tgggcttttt taccgaactc gccaaaatct
    49861 atcccgattc cttcgagctg aggcgccacc tgctatgcaa caaatctgtc cgtgcaggag
    49921 gctgggctct ctatgatgaa catacggttt ccatttttga caccgaaacc aaggtcctga
    49981 agagacggac agtacaagcg ggcgacgtgt tccttttgta tgaatggctg gactatatga
    50041 tgtccgtcag caacaatggc gctgccaccg tatgctggag agaggccatc ctcatgcgtg
    50101 tttttggaaa ggaatatcct gcgctcagtg aagttcaggc ggaggcctat tttaacaaga
    50161 ccccgaagtc cgaactttca gaaaccgcga tctccgtcgt caatgaaccc ttaagagcaa
    50221 tggggatcac agccgatgaa tggcggctgg gcaccatgtt tacgcgcggc ggatcaggcc
    50281 gtatcccgcc gaagggagga agcacaggca ccccgatggg actaatgaaa ttcctgatcg
    50341 gattggagcg cgggaaactc gtggacgaag aaagcagcct ggaaatgaaa aaactcctgt
    50401 acatgaccga ccggaggata agatatgcgg cctccccggc attgaaggat gcggccgtgt
    50461 attttaaatc cggcagtctt tacaaatgcc ggcctgagga aggttatgtg tgccagaaat
    50521 acaaaggcaa tgtggataat tttatgaaca gcatagccat tgtggagcat cccgaccaaa
    50581 ccacctacct ggtagccctg atgtccaacg tacgaaagaa aaacagtgct tccgatcata
    50641 tggctctggc ggcatccatt gaccgagtgg tccggggaga ttgagtttat acgtccaagc
    50701 gcctgatttc agacaatctt tccgctaata atcggaccct gtaatgctca tctccggttg
    50761 ccaaatggat cagggggtca aattcgggat tgtccatggc ttcgatcagg ttcaacaccg
    50821 caagtttcag acggacctcc ggccccttca gcagggattc aaaacaggca tattcatccg
    50881 gtgattttaa ttgcagccgt ttgagggcct catccggtaa atgttcaagt gcggcggtct
    50941 ccaccagggg aatcaccact tttttcaggg aaggatctat gatattgtcc agcaattcga
    51001 ccgtgttgat gcggatatta gcgtcgctgt tgcggatatc tttaaatagg ggcagaatga
    51061 tgcccggagg ataggtcagg cccagcaacc agaaaattct ttttaaaatc cggttcaact
    51121 tgcgttccag gaggcgggta aattcttttc tggcaaggat aattccttca ctttcctggt
    51181 ccgttttcca ctggtgttgg gcggcataat tgatggccag ggcatcccgg tacaggcctg
    51241 cttccctcaa gagatgaggc accactcgtt tgccgctgat ggtcaggtgg gggaaattga
    51301 ccttcatttt atggagggtc tgtatggctt tcagttttac cagggggtcg cggtgttgga
    51361 caagctcaaa caggaagtca atggcctgtt gggtatccat ggttttggcg agggcgggca
    51421 attggatcag gatctctctt aatgtacttt tttctatgct tacatccgtg agtataggca
    51481 ggatttggtg gggttcaaat tgggccaggg ctttttgggc aaccgaacgg gtggatttat
    51541 ccggcaggaa ggccaataat attttaacaa atacagggtc ctggctgtaa cctgctgcca
    51601 ggatggcctt cccggatacg gcaggatttg cgtccttcat taaatcagcc agtaccgaat
    51661 agagagaggt cagcttacca tatccgatag ctctcgcgac catcattttc tgcgcttcct
    51721 gttctttagg atccggaaac cgggcactgt gctcgaatgt ttcacggagg cgttgttcca
    51781 ggttgaattg ttgttgcatt tcaaggttat cacgcgcttc ggtagccagt ccaaccaggg
    51841 cggcccggtt gatcgcggga tccgggtcct tcagtgattc ttcgataaag tgcacccggc
    51901 ggtcacgggt atgggcgagg aggcaggaaa aggcccgcgc gcggacttca tcgttggggt
    51961 ctttgagcag gggctcaata tggttgatga cggagtgatc ggtatgataa tataggcaac
    52021 gcaaggcggc ctggcgaatt tccggggatt tatgggttag cagggggatc acttccggca
    52081 tgatgctatg gcctttgatc tcttcgagcc gttccagtaa gaattgaatt tggtgtacgg
    52141 aacccgattg aagggtgcgt tttattccat ctgctacggt ctcgtcggag agctgaaaat
    52201 ttttctttgc ggatatattc gggttgaggc ccagtatttt ctgaaatgcc aggacatatt
    52261 cctttttgac aagtattgta aaatagatcc atagcccagc aagcaggatg atcattatac
    52321 aaacggcgcg gaccgaaaga tccagtccgt taatcaggaa tatgagcgtg atgcccccga
    52381 tgcccgtcgc cgtggtatcg acaaaaacat cgatgaaggt ctttacctgg tttttaatcg
    52441 ccggtggaac aggcatgatc aggagttccg tagccgcttt attgatggat tgtttcaggg
    52501 aaatatcagt tagtttgagc agggtacccg tccacaggac cggggtataa aataccgtgg
    52561 cggctcccgc aaaaagagcg cccggcagca caaacagact tctgcccact cccagatatt
    52621 caaccacttt acgggtgatc aggagctgaa tcaccaggga caccatattg gcggtggaaa
    52681 accagaaacc gaaaaaagcg gtcagctggt cagggtcccg gatcttcgac gaagcaatcg
    52741 cgctgtattg aaattcaacc aatttggcga ccaccacgct gatccccatg atcagggcca
    52801 ggaaggtcag atgttttgaa ttccgtatca ggcgcaaagg gtattcatgc agggctttcg
    52861 tttgccttag ttgaaaggag cggttgtatt cggggataaa ccgcgaccag atcctccggt
    52921 tcaccgccat gctgatgatc aaaagccctg ctgcataaaa gaggaggttg atgctatccg
    52981 acacggaggc cagcagggag gtcacataac ccccgacgat ccctccagca atcgctcctg
    53041 caccgataat gccaaaaagc cgtttggcct ccagcgaact gaataccata ttcccgagaa
    53101 gccagaattg ggaggtcgtg atcagaccaa acagggccac tccgatataa atcaggtaac
    53161 tcatccagtc tttgaacaaa tccaggcgca acaggatggc aaaagtgagc aggcaaaaaa
    53221 tggaaataag gtgggtgcgg gtgatgagcg cgcccagggg gatccggttc aaccaccggg
    53281 aataggccga agcaaccacc agggccgtca aagctaccaa aaggaagacc aggggcagtt
    53341 tttcaatgcc tgcacgggac aggaattggg cgttcaccac gggtttaatg atccacaggc
    53401 tctggatgat caggaaaatg gtcagctgca tcagccaaac ccgcctgaat tcgccttcac
    53461 ggatgtcaaa aattcgtaaa tgaaagtccc tccacttcga caaaagggct tcaagcataa
    53521 tttatgctaa gctagccgtt tgccaccaat gcgctgtcga ttttgagcat caggtcccgg
    53581 agaagttttt ccccctggct gtccatggcc agggctacca cgatgtatcg gcggctgtca
    53641 tcccaaacca gggcggaatc ggcatgccag ttttcccagg tgcctgattt gcgatatact
    53701 tttgcttttg gagccacccg gtcaaggacc gaaacaaact tgtggtggag ttgtggatcg
    53761 accaggatct ggagcatatc ccgggaccgt tccggattga ccaactgacc aaaggccagt
    53821 ttataataat atttacaaac ctgttcgacg gaagcggcgt ggctcaggtt tttaagcgga
    53881 tcacccatgc gggtaccgcc gccgccatag ggtttcccaa cccacaggcc cccgccgttg
    53941 tccttatcgt acaatttata agaggggtgc tggaggaccc gggcgatttg cccaatgccg
    54001 accctttgga tcattctgct ggcagcctca ttgctggatt tggcaatcat ccatttcata
    54061 tcctgtttta cctccggggt ttcctgcagg gtgccctggg caatggcctc ttcagaagcc
    54121 agcaggacgg cgattttggg caggctggcc gcatacatca tatgattccc gttgatagcg
    54181 gcaaaccgcg aattcatggg gtcgtacata tcgaccaccc cgatactcag ggatttattt
    54241 cttgccaact ccatccaccg ccggtctgag ttcaggatgg tttccagttg gtcttgcaac
    54301 tcctgatcct taagggcttc aagaggtttt accacccctt ctggtacggg gagcgggaga
    54361 tggtccaaaa ccggatgccc gggatttgct accgcttcgg ccttgccgac cggatttccg
    54421 ggtaatgagg ttgaaggaac cgaactccag gaagtgaggg taaagaaaaa aacaataagg
    54481 attgaactca ttgagatcaa cagggaataa gcggtgaatt ttttcatata aatacttcat
    54541 tctgcttatc aggcagataa tacaaagtta accccctcaa aaggaaaaag gacaggcttt
    54601 gatgcttgtt aacatccgcc tcatttttta agccggccat aactatggct gttttttttt
    54661 gaggttaaaa caattttccg ccgcattttt ttaacgagcc gctaacgtat ttttgggcgg
    54721 gtggagataa agccgaaaag tccaggggaa aagttaaaca acctgtatta aatttgaatg
    54781 tgtccaatag ccatatgacc ggtttgaaaa ggcattcttt gaggatgtat gtcttcctgt
    54841 tgctcaccgc cacaaaccct ccgttcacca acgggcaaac ctcgatctcc aaatggcaat
    54901 ttgggaaaca aggcgcggta tccattacct atgacgatgg ttccatcaat cagttccgta
    54961 aagcgcttcc aatcatgaac catttaaaat tgccggctac cttttttata attaccggac
    55021 agatacccgg atcccaatac cagggaaaat ttatcggtcg ccccgtaagt gaaattatcg
    55081 aagagtcttc aagcgtcctc accaataaag acaatttttt tgaacgggct tcggcagcgg
    55141 gcttcctggg ttacagaggc accttagcct atcatacgcg ggcgggggct ttgtacgatg
    55201 ccggaaaaga agaagaggcc tatgccattc tggatgacct ttatcaaaaa gtacggaacc
    55261 aggagtttct gcctgacacc ggaacccggt acggggagat tccggcggat agagtcacct
    55321 gggatgaaat cagaagcttc gcagcccaag gacatgaatt tgccagccat acggttaccc
    55381 atccttattt atgcgcactg gatgaggtga acataaatta tgaactggaa aaaagcaggg
    55441 atgaaataca aaggcagctt ggcccggaat ataccttttc ggcggagatc ccttacggca
    55501 cggaacatga gcgggccatg aagtatgcac accgggtatt ccccgccctg agaaaccgga
    55561 tgcccgaaca ctacctggtt gaactcaacc gagccagcac caaaaaccct gtagatttta
    55621 cggaagagta tgtgcagtgg cagcggggag cggtggccag gacctccttg cccatgatga
    55681 agtcctggat cgacaccact cttttaaaag ataacatttg gctggttctg gtaatccatg
    55741 gagtggacgg tatcggatgg gaagcgctga gcagtgaagt cctggatgaa tatttccgtt
    55801 acctgaaatc caaagaaaac catctctgga tcgcgacgtt tggagaagtc accaaatata
    55861 tgcggagccg catgaacgcc agggtccaat cgaaaagcca ggatgggaag ataaccgtaa
    55921 gccttaccca ttccctggac aaatccctct accaggtccc cttgacctta aagacttatg
    55981 tgcctggcca ttggaacgag gtcaggataa aacaaggaaa tgaaatcaag cggatccggc
    56041 aacaacttga ttccgccggc gcctatgtgc tttatcaggc ctatccgaat tcggaagaga
    56101 tcgaactgac cgcaaattag gatgccatgc cgccctcaat cctttaccat ataaaataac
    56161 taccatggcc gaatccaata acccgagcag ggtctccgga cctccccgca tcgcttcaat
    56221 tgatattttg cgtgccctga ccatggtcct catgatcttt gtaaatgatc tgtggtcttt
    56281 gaagtccatc ccggcctggc tggggcatgt ggaacaagga gtggacggca ttgggctggc
    56341 cgatgttgtt tttccggcct ttctttttat cgtaggaatg tccctgccct ttgccattga
    56401 caaccggagg aaaaaaggag atacagggat acagatcacc ggtcatatcc tcctgcgcac
    56461 gatttccctt ctggtcatgg gggtattcct ggtcaacggg gaaaccttca atgccgcagc
    56521 caccggtatg gcccggtatc tgtggaatcc aatctgttgt ctttctttta tcctgatctg
    56581 gaatgcctat ccgccaacga caggtaaggc cttgaaatac ggcttaaaat caataggcat
    56641 gattgcgctg ttggtgcttg cgttcattta ccgcggaggc acggaagggg acatctaccg
    56701 attttcaccc cagtggtggg gtatccttgg actgatcggc tgggcttatt tggtcagcgc
    56761 cctggtcgtt ttgtggtcaa aaaacagatt ggcctacctg ctgctggcct ggactttttt
    56821 tgcccttctc agtattgccg ctaaagcgaa atggttttcg atcgacgcct tgtcctttat
    56881 cccgggggct atcagcggcg gaacgctgac gggtcttgcc ctgggtgggg ttgtcgcttc
    56941 gcaggtgttc aggtattacc gggagcgccg ggacgaccgg accatgacct acctgtttgg
    57001 tgcggccatc gtaatcctgg tggcgctttc ggtccttacc cgtccttact ggggcctggc
    57061 aaaactggga gccaccccgg cctggctgtt tttatgcagc gcttttaccc tgggcgcttt
    57121 tgtaatcatc tattggattt cggatgtata cgggaaagcc gcctggttta acctgatcag
    57181 gccggccgga accgataccc ttctctgcta cctgatgccc tattttgttt attttattct
    57241 gcgggtcttg catgtacgct ggcctgaggt cctggttaca gggggcgtcg ggctcgtaaa
    57301 atcatttttg ctggccttgt tgtgcgtatg gctgaccagg attttgaatg gtgtaggcgt
    57361 gaaacttaag ctgtaaggag gtcgatccgt cggtgaagtc atcctgcaaa tagcgaagaa
    57421 agtgagaaag ggagaaaggg agaaagacga aggatgaagg gagcgaaagg cctcaactcc
    57481 cagctcccag ccattttatt tttccttgat atcctttccg agcctttgtt gcaccaggtc
    57541 caccggatat tgttttgaaa aatcatgtac ggcaaaccgg aattcttcaa aggcttttgc
    57601 ccaggcttcc gcttcctccc ggctatattc ataaaagcca tgtccgttga aaattccttt
    57661 accccctttc tgcacgatct tttccataaa agggggtacc tcggacaggt tgctcaattc
    57721 gggaaacagg tctttcatca catgataata ggcctggaac ccggtcaggt ccatataacg
    57781 gaaaggcccg caaaaagtca tccagcggcc ggcatcgttt ctgcaggccc tgtcgatgtc
    57841 ttccaatccg gtgtaaccgt tggcaaccag gtagagcgct tcccggtaca tcgcgtacat
    57901 gagccggttg gtgatgaatc cgcggatgtc tttgcgcagc agggtaggtt cctttcccca
    57961 ggtcgacgcc agaagataaa gggattcggc aaccgacggg tcggtatctg gcccgcaaat
    58021 gatctccaga tatggactcg taaatgcagg ttccgcccaa tgcatgccta aaaagcgccc
    58081 ggggatttta aggttggctt gtaagtccgt gatgggtatg gctgaggtat tggtggttat
    58141 gatcgtttct gcggacaccg cctcttcgat cagggccagt tgcgattttt taacttccag
    58201 gtcctccagt acgttttcgg agatgagacg gcagggctcc aggtccgcat gatgctttgt
    58261 gtaggagatc ccggataaaa gatcctccac attccggtca tggatcccct gtttcctggc
    58321 ttcatccagg ctatgccgga tgcgcccggg agcggtatca aggtcggatt caagtccggc
    58381 caggccaacc actttatagc cccagatcaa aaatgcagcg gcaatactgc aacccatata
    58441 acctaagccg gccacaccca ccgcgttgga ttccgtattt atcataaaac ctgatgtttt
    58501 tttgatgacg agtttcaggt cacttgctgg taaacttatc aaaaacccgg gtcagggaga
    58561 aacatttctc accccggcat tcgataaaaa tttcaagggc agtccaaagt cctgatttca
    58621 tgtggggaca gtgtaaacaa agccccttca aactcagcca cctgggtggt gtttgcatag
    58681 ttgatcagga tcagtttttt ccggccctct ttatccagga ggagggcatc caggatcaag
    58741 ggttggctgc tcagggtttc aatgagctcc ccacaggatc caagccaggt tttcaaagca
    58801 tgaaacacgg gataagcctc gccctctgcc gataataggc cttggtttcc cgcgctttgg
    58861 tataaggtca cagcctcaca tccaccgagt gcgagtcttt tcaggctgcc cagggcatag
    58921 gctgcaccca ggaatgtttt ctgccgggga tccgtttttt gctgattggt aaaattcctt
    58981 ttttccggat ccgtggcatt ggggttccgg cgcatgcgta aagtgattgg cgagatatga
    59041 atgcccatat ttgcaccgaa tttatgtttt gcggacagca ccagttcttc caaggacccg
    59101 gtattctcta taagggtacg gtcatcaaat gcatgtacct ggggattgac ggaaaatgaa
    59161 acgaagtcca ggccgctggt ttccatccgg tgccggttta tctcggtaaa attaaaatca
    59221 gtgcctgccc cggttttcgc ttccgtaaat gccgaacgga tcaccggaag gcaagacagc
    59281 gcgtccggtg aagtcaccgg atggtcttcg ctcaaaagga ggatgtattg aggccgtatc
    59341 cgatgactat ggcaaaagcc gataaaccgt tcaatctggt gcaggcaagg ggccgtcagg
    59401 tgaagtgcaa aatagggtga acaaccgagc gaaacactgt tttgatattc cctcaggcct
    59461 tcggcttccc aagccggccg ggagagattc aaatcaatcc ggtaatggtc gagttgaagg
    59521 ctgcgtaaga ccgccagggt ttctggatcc aggcttccga tctccgtgga tgccccggtc
    59581 ccgatccttg gcgtagggaa gcggcgatga gtggatctta ttttaatcgt cgggtccggg
    59641 gtgcgcaagg cagccagggt ttcgaggggc ttgaactgca ctttttgaaa aacagtctcg
    59701 cccgtatgga gtttgaccgg gaagggcagg gacaatgggg tgcaaaacgt cttgaaagaa
    59761 gcgtcagacc agttgcgctg atcttcggtt tcgaacaggt ctccctcgaa gtccagggaa
    59821 taccaacggc ccccggcttc ccattgaaag ccccggatca acttaaaggg gtttaccgga
    59881 tcgacctggt caggaaaaaa agatttgctc accgctcccg tgggatgtaa tattttacaa
    59941 ggttggcgtg cggcgctgat cgggtggagg atgcaaaagc cggccctgtt ttttaaaaga
    60001 tccgtgaggg ctctgccccg gatcataaac tccagggagc cgtctgcaaa tccggaaatg
    60061 ctgacccgcc attggaaaat ccgcctgccg tctttttcgt gataagcatc gtattcaatg
    60121 gtaaaatggt cggcttggat atccgttacc tcctgttctg tctgaaggga aagggttgcc
    60181 cagttttcat ccctcagggc aaaatagatc atgcggagaa tctcggtttc gccgtaagtt
    60241 atataacgca gaaacccgga ttcgtagagc atttggaccg gtccggcatg gaggatcata
    60301 tcgttttcct gtaaaactaa aaccagtccg cgaacctttc gaattctttt attccccggc
    60361 tctgtgaagg tgggttcgca gtatggccat tattgtataa atttgaaggc ctgaagcaac
    60421 aatgaattag tatgaaattg aaatccattc tcgccgtatt cctgtttttt tccacctttt
    60481 ccatccctgc ccaggaaata ggcatcatca tgggcacggc ccgccacatc atgcaggagg
    60541 atgtggagca aggcctcatc cggctgaaag aactgggtat ccggcacctt gaaggggccg
    60601 gttccaggac catgagccgg gaagaatata aaaaactgct ggacaagcat ggatttgacg
    60661 tagtggccaa cggggtcaat tttgaacgac tggagcatcc ggacagcatc aaaaggatca
    60721 ttgacaacat gaagttttac gaagcggaat atgcggtttg ttactggatt ccgcataaag
    60781 gggatgattt cactttcgcc gatatgcaaa agggcgtgga ggtcttcaat agggcgggca
    60841 aacaatttgc cgaatccggc atttcgcttt tataccatcc gcacggttat gaattcaggc
    60901 cttacgacgg gccgggcacc atgtttgact acatgatgga aaaaaccgac ccgcgttata
    60961 tcaattttga aatggatgtt ttctggatcc gtaaccccgg ccaaaatccc gcggcccttc
    61021 tcagaaaata tccgggccgt tttccgctga cccacctgaa agaccggctt ccgggatcgg
    61081 tggataatcc caacggccgc caggatcccc agcgtaacgt agtgctgggt tcgggcgatg
    61141 tcaatattgc cgaagtcatg aaggccgcca gggaaacggg ggtgaaatac cacctgattg
    61201 aggatgaatc cgcccgtgcc ggggtccagt tgcccatgca tctcaaatat ctccgttctc
    61261 tcgacctgga catgcaggct gtggaattat ccgtcagttc gctccgcaag gctatgctca
    61321 gtgcggattc cctggctctt cgcaatctga cctgtgaaga gcttacctat ggacattccg
    61381 gcggattgat cgaaggccgc gaaacctttg tcaacaacct ggttaccaaa aagaccgact
    61441 ttatcaccat ggacctcaaa gaccaggaga ttacggtcaa gggggatgtg gcctgggtgc
    61501 gccacctcct ctcggcggag atcgtggaca atggaaataa aaacaacgtt aacctgaaga
    61561 tattattgat ctggactaag gtggacggga tctggaagtt gctggccagg caggcggtga
    61621 gggcttgatg gagggaatta aataattaaa aaattaaaga attaagaatt aaggattaag
    61681 ggggggggag tttgtcggct attccttgat tattttattt acccttacct ggtttccata
    61741 gaatacccgg atgaaataaa tgccatccgg ttgagtgctg aggtcaatgg attgcgttaa
    61801 agggccttta aaaaccgtct tgccctgaag gtttgtgatt tcaatgctgg tgcggccgga
    61861 taataaggca ccatcggtct ggatttgaaa tattccccgg ccgggcaccg gccagatttt
    61921 tatttttttg ttttccggta catcccggtt ggaaaccgtc agcctgccca ggcaggaaga
    61981 ggtatccaca cagccgttct tggagaggat cactgcatag agtccgtggt cttcaggcat
    62041 aaagcttacc gaactttgac cttccagggg gaccaaacca gggcaggaaa cccattggta
    62101 agcggccgtg gtgtcccggg ccatcagcaa gccattcgtt tgaaggacag cggtatcgag
    62161 gcttagggcg tttaccggca gcatttttgt ttccgaagag ccgcagaggt tagacgcgaa
    62221 aaccatcaga tgacctgagg acgtacccgc taccaggttg atgatggaac tgtgggattg
    62281 accctgccac cctgccggaa caatccaggt ataatccgta gctccgttgg ccggcgttat
    62341 ctggtaggcg tggatactgc ctgaacacac attggtgtca ccggtaatag gtccgggaag
    62401 agaggggatg gagttggccc ggaccaacag gggcctgaag gaagaagaac cgcatgcatt
    62461 catagcggct acggtcaaaa taccgcttgt attgcccgta atgagggata tcgaagttcc
    62521 tatagagtta ccggaccagc caaaagggat ggaccattcg taagccgtag ccccgtttac
    62581 aggctggatg ctgtaggtca gggaacttcc cggacagacg ctggtatctc catttatagg
    62641 acctggaaca gccggtgttg accttacggc gacggccagg atccggatcg cggaggcacc
    62701 gcaggcagca ttcgcggaca cggtgatgtt gcccccggtg ggtccgggca caacgtttat
    62761 cgaagtgctg cttgaacttc ctgaccaacc tgccggcaag gtccagttgt aggaatctgc
    62821 gcctgtgact gctgcaatgg tatatgtttt ggcatttccc tgacaaacgg cggtatcccc
    62881 gcttattgac cccggcatcc cgggggaagg ggtcacggtg accacaaggt ttcttgcctg
    62941 tgaaaggccg caggcgttgt tggccgtcac ggaaatattc cccccggaag tcccggcggt
    63001 gaccgtaatg gatgatgtca tcgaattgcc ggtccagcca tccggcaaaa cccaggcata
    63061 ggaagttgca ccactgatgg taccgatatt ataggtattt gtactgcctg aacaaatggt
    63121 ggtatttcca ttgatcgtgc ctggtgttgc cggtgggctg ttgacggtta ttgccaggga
    63181 ccgcactgaa gacgatccgc aggcattatt ggccatcacg gtgatcgtac cgccgccggt
    63241 ccctgcagtg gtcgatatgg tattggtcat ggaacttcct gtccatcccc cgggtaaaac
    63301 ccaggtgtat gaatttgcgc cgctcacggt gtttacactg aaaatattgt tgctccctga
    63361 acaaatactg gtattgccgc tgatcaatcc cggggtggac gggctttgat ttaccgtgac
    63421 tgtgaggatt cgggcctggg agggtccgca ggcattgttt gcagtcaccg taatgtttcc
    63481 attgttgggc ccggcaaagg tattgatggt attagtgacc gaagttccgg accatccacc
    63541 gggcagggac caggtgtatg aggtggctcc gttcaccgga ccgacactgt atgtatttgg
    63601 acttcccgaa cagacggtgt tgttgccggt gatcaggccc ggtgttgagg gtgggctgca
    63661 tccctggacg acgatggtcc ctttcattcc ctgggaagca tggggcacac agacgtaata
    63721 atgggtgccg gcccccagct gggcgggcag gaccaaacct ccgccaaatg gaagctggaa
    63781 gcctccggca agggagctgg ttccattggc attccaggtc ccctggctga cttcccggac
    63841 attgtgcgca acggcaagat taaattgcac actgtcgccc gggctgatgg taatcgttgc
    63901 aggtgaaaaa ctgttgcctg aattggttac gaaccaggtt ttgcagaatc ccggaagaga
    63961 ataaataata aggcaggtaa aacaaataat tttcatttta ataattatga gtcccggtaa
    64021 tttgattttg gacagaagtt ctctgccccc tgcaattctg gtccggaggt tcttggtatt
    64081 aaaagattaa cgactcccgg attaattttg tgacttcgcc cggaccgaag cgctgttaat
    64141 atttaaccac gcagggactc cattcattat tatttacctg gaaataaaca gatttaaagc
    64201 acatagcggc gatcctgtag ccgatgaagc cgtattcatg attttggata aaatggatta
    64261 aaataaaccg agttgattgg acgaatgaaa gcccgtaaat ggggtcaggc aatgctcatt
    64321 ttgtattcca ttattttaag cacatccatt aattttttct tattgttcac agcaatgacc
    64381 aaagggacca gtttaccgat gggtttaagg gtcccaagtt cattttttat tttttgggcg
    64441 atatccaaag ccgcaacgcc tgaaatggtt tttgacgtaa aaaagaaact tgttttaaaa
    64501 cccgtatccc atatggacaa ccaacaaacg gtcctggatt ttacgcaaac cttgcacagc
    64561 caagctttgc catccttgta ataattccat tttggttcta aatggtattt ttcaccggtc
    64621 actgtttgaa taaacgcttt aaataaaacg aatggtttac ccagcgcttt gcctaatacc
    64681 tccgctgaag gatagacctt ctcctctctc aacaactggg tttccatgtt tttcatagtt
    64741 aataaggatc cttgttttta acccattgaa atcccaatga gtttcccaat cccaaaagtc
    64801 acggttgcgg cagccaaacc aaaaaggacc tgcctgaagc cggaaaacca gacatttctt
    64861 ccggtaaaca aggtgatggc cgcaccgatc agaaacaagc cggctgcact ggctgttacg
    64921 ctgaggacaa tggcgggcat gccgctggag aacatgaatg ggatcaccgg aacgatggcc
    64981 ccaagggcaa acaggataaa ggaataaagg gccgcttcca ctgctgaacc tttcaattcc
    65041 tccgcattga tgcccagttc ctccctgacc agcgtttcat gggccagatc cttattttta
    65101 agaagctctg atgccatgtg atgagcctgt tcctcgggga tgccttttgc catatagatt
    65161 aaagccaatt ctttcatttc tccttcagga ttggtctcca gttcttccat ctccagtttc
    65221 atctggtttt cgtacagttc ctgggaactt ctgacagaaa tccattcacc gagggccatg
    65281 gataaagctc ccgccaacag gcctgccagg ccagccagca gcaccccttc ctggccggag
    65341 gttgcgccgg cgatgcccat gatcaggctg aaattggaaa ccaatccgtc attgcccccc
    65401 aaaacagccg cccggatggc attgcctccg acagaacggt ggcttttttc cattttggag
    65461 agttgggcgc cggtgacgtt tttttccttt tccaggatgg accgaaggat ggttacatgg
    65521 gtggtttcca tgccggtgat ggcctgttta tttttttgtt tggtggaaat gacggcattg
    65581 gacaggtttt tttcggtatc catcaggaat cccagcacat agtcataacc gaagactttc
    65641 ccgatcacat tcatcgtctt ggccctccag gaaggccgga gcagggattc aaaggggagg
    65701 ttctcctttt tggcaaaagc ctcggcatgg cttttttcta tctcgctcat ttgcctgaac
    65761 acgtttacca gggtcggatc ggattcatgc tcagccagtt ttagataaag aaaacaggcg
    65821 tccacctccg tttgtacatt attttttttc atttggcagg aattgcattt ctgaaattac
    65881 aaaatacgcg ccaaaggtct tgaaagcgat gcctttaagg gcgaacaggc attcccgagg
    65941 aataaaaggg ggagggcctg aacctatccg ccacttggga agtttgccat ttttaattta
    66001 acgttaatag ctacaatgat catgaaaggc tccttatcct ctggaaaaat tccattcacc
    66061 ggttcaggat cggtaaatat tgtatccagg ggcccacttc cctggtgtat tgcctggcca
    66121 tgactctggc tatttgtgca atatggctaa ggtcatgggt ggtccaggtg gcaagcaatt
    66181 ggtcaaggcg tacttctccg aactccgggt ggatccccgt cttggcaagt tcccgtgtat
    66241 caaggttttt ggatttcaga atccggagat tgttgctgcg cagggattga aattcatcca
    66301 acaactgccg gatatttttc cctttactgg cctcgaattg ggcaaaccgg tcaaaaggct
    66361 cgaattttct gtcggggcca ccggagagga tgatctccat tctggggatc cagtctgtcc
    66421 ggtcgccgtg gatcagatga ccgaccacat caaaaggact ccaggtcatt tccccttcat
    66481 tgttgtaaat ccactcctcg ctcagtccgc ccagtagggc ttccaataac accggggtgc
    66541 ggcttaggat ttcaacgctt ttattcagat caaaattcat catttgttaa acgcttacaa
    66601 gaggcaatcc tcgattcgat tacccacgca tactttatta atcattgagc cgccctctcg
    66661 tatttccata atttattggc ccaggggatg aagtatttaa aatcgggtgc atccgtatga
    66721 ccgccatcgt gttgcctcca ggccagctcc ccatcgagaa gcccttccag taccgggggc
    66781 atggtcgcgg tcctgtattc gtgcgatacc cccaggtccc ttgctcctaa aagtttgtat
    66841 acaggcccgg ctgccacagt ggccatatag cttccctgct gatccagcca acgggcgtcc
    66901 cctttttcag gtatgccata acttacaaat accggccttg gggcgcagag ggcaagcaat
    66961 tcgtgggaat ccacggaaag atcacaaccc gtcttgcttc caaaatcagc ttccgccgcc
    67021 ccgtatttca tatagttgcc ggccatccag taatataaac caccggtcag gttttccacg
    67081 gcttcaccaa acactctgcg atgcagggtt gcaccgcctt tcccggaaga gccaatcagg
    67141 ccggtggcaa agcggggttc aaaggccagg gtgaccaggg ccgctttacc ataacgcgag
    67201 accccttcaa tacccacttt tttcgcgtcg accagggtat ccgtctcaag gtaatcgagg
    67261 ccgcgcgccg caccccaggc ccaggcccgg agcgcacccc agtcttcggg tttcctggct
    67321 tgacctttgt tgaccaggcc aatgatcccc cgggttagtc ccgcaccgtt atctgcctga
    67381 atgcttgcgg ggtcaatggt ggcatatccc caaccggccg ccaggagctg ttccgtgggg
    67441 gggaggtccc cggcgggagg cggcgcaaaa aaattgggag ctggtaagcg ggtgacgggc
    67501 gaataagcgg gataacgatc gaaaatcgct ttcaatgaag gatcggcttt gatcatcatt
    67561 tctttaaaaa ccgcgttgag tttttccatg tcttcagccg ggggctgtgc cggggaaggg
    67621 aaggccggcc gtccgaacat catcaaaacc ggcaccggcc cctttacatt ggcggggacc
    67681 accaggatca tgttgatgtc aacctggatc agtgggtagg cgctgttgtc cacatggcca
    67741 atcacctgtt ttgctatgac cggtacacgg ccgacaaatt cgcggtccgt gatttttacg
    67801 gtccaggtca ctgcagggat atgttctgga agccgtccat atacttcttt ctcaaagtcc
    67861 tccacaattt caggccggcg ttgtttccac cattgttcag ctgaggacac tttttgtccc
    67921 gaacgggtgg tcaggatttc cggtaattga gggcagggat tggcttttga ttcgtcatag
    67981 tttgcatggt tgggagcgga ttcgttgccg cttggtccgg gccttagttt ttttatgccc
    68041 agttggcgca tcatatcatc atggtcttcc tgggcggtca tggttaccag cggaggccat
    68101 tggccaccct tattcgcctc ctggccgatg gatgggtgaa aaaaccacat agccatccat
    68161 aaaaagttga attttttcat gtttcaaaaa atggtttatt ggaattttat ttttcgctga
    68221 ctggctggtc ggtcctgaac ggggaagccg gcaatccttc ccggttgtat agattgggat
    68281 tgaccgggtt gtccgcccag gcataacgca catgtcgtgg gttagggacc tcctgattcc
    68341 agaccatcaa ctcgttcccc cggatcaccg cctttgccca aacaaatttt ttgtcctcgc
    68401 ccgcgatggc aaaatcgctc agctcctccc cgtcaaggga tatcaggccg ctgccaacct
    68461 gggtgaaggt gatcctgatt ttatcgcctt cgatggcatg ggaatggtaa agggggccgg
    68521 aatggaccaa ggattcacca taagcgattt tcagggcggc cagggccatt ctttccccga
    68581 cgtctttttt attgtccgga tgaatgtcgt tccattcacc caggtcaatg tttaccgtca
    68641 ttgcggtatt gggcaggctg agggcctttg tctgggattc cctcaattcg gcccagttgc
    68701 tttccgaagg cagatagtta taatccatat aattgggcag ctgggcatag atgaagggag
    68761 cttcgggctg attgaattgg ttgcgccagt cccggatcaa agtgggcaag aaacctgcat
    68821 attctttggg tcgtccggca ttggtctccc cctgatacca aaggatccct ttgatggggt
    68881 agtgggcgac cggggccacc atggcattat acagggcggt gggctgattc tgggcattga
    68941 tcccgcccgg cgacggaggc cggggtataa aaacttcgcc cactttgtat ttccagtccc
    69001 ccttgaggtc aaccgtatcg ttggcggtga aaatgcaata aggtttatca ggcacaaaac
    69061 cgcctttgcc gttgaaattg gtaacccgga tgacaaaggt atttttacct gctttcagaa
    69121 gtccggcggg gaccgaatac cttctttggg gatactgata ggtgacctgg ccgacttgtt
    69181 taccgttgat gtataaaata tctgcgtcca cgatgcggcc gagccaaacc ctggctgctt
    69241 tgccagccat ggcagtgggc agttcgattt ctctccggta ccaaaccacc ccattcagat
    69301 ctttgacgcc ctggtcttcc caataaccgg gaatatgaat ggtccgccaa gccgaaggga
    69361 ggtagtcttc ggcaaaccat gtttttggcc ccgtaagccc cttatcctgg gaaggccttg
    69421 cggctggatt gtttggaggg gctgccctcc ggttcatttc atggatatag gccgtgtcct
    69481 tgtttttatt aatggtctgg agaacctggg gaaattccct gaacccctct tcgctggtcc
    69541 aggcttcgat cggagtgccc cccacgctgg cattgatcaa accaatgggt atcctgtatt
    69601 tttcatagag tttcagggca aaaaaatagg ctacggcgga gaagggcctg acctcttccc
    69661 tgaccgccgg tctccaggag cctgcggata agtcttcatt cgggccggaa aggctggtcc
    69721 gcgttgggat aagaaattgc ctgatttccg gctggttcgc ggaggcgata tcatgggcat
    69781 aagtcacatc atggatgttc atctggtgga ccatattgga ttgtccggaa caaaaccaga
    69841 cgtctccaaa caggatgtcc ttcagggtta tgtcgttttt accggaaatt ttaatgaggt
    69901 gggggccgcc cgccttcatg gccggtaaaa cgagtttcca tttgccttcc ggggaggcgg
    69961 tggtttcatg gcgccggccg tcaaagataa tgctgacttt ttctcctgcc gatgcccaac
    70021 cccaaagggg aatgggctga tccctctgca ggatcatgct gtcccggatg atttgcggaa
    70081 gcctgatctg ggtaaaggag ggcgccggat tcagcaataa agtcaaataa aataaaaaag
    70141 ggcgccggtt tacgctccaa ggcatcctgg tcataaatcc atttgattat ctaagtttta
    70201 aagcgaatac cctttcgggt gttcaaatcc taatctatgg ataaaaatag gaaaaattaa
    70261 aggttaaggt atcgggggtg tatgacaaat tgccggttta cactttagga ttgttctatc
    70321 agggatgaaa ccccggccaa ccgtatacag tgcttcttct ttccgggccg gctctccggt
    70381 catgttatac gaaggaacca gaacttcaaa ttggtaatta cagcctggta agttttgtaa
    70441 aagcctaccg gatatttaat tctttataaa atttcggaag attataaaga agcatgcaat
    70501 ccggttcatg acagggtagc ccctaccaat cccgtaacga accatccctg caaataaatc
    70561 caattccttg gacttggcta agcagaaaag gcaggtccgg acaaatgacc tgctggtgac
    70621 ccaggccccg gcaacagatt aaaatctaca gccgtcggac tattgcacta ccatcacttt
    70681 taaaattctg ttggtcccca ggttgacgtt attgaatgtt ccgttgtaaa tggaaacttt
    70741 tatcattccc ggctccgata caaaggtggt catttgacaa cctttaaggt ccgcgggaat
    70801 ggaccctacc acgatgtttc ccagatccac tccaggtatt gcaatgggag gggacagcca
    70861 ggtggtattt ataaatataa tgcccgggtc tattgtgtcg gcgacggtgc tgatgatgtt
    70921 ggcaattccg gaggaaatga tcaccgagtc gggggtgact gaaactttga tgccgtctgt
    70981 tccggcgagg actttcaatt taggcaggga atcggtgacc ccgacgggga tctgcccatc
    71041 ggtggctttg cctaaggaac gcaaagcccc ccggtcattg cctatgatca gcgaattggc
    71101 ggtcagggta tcggtgcctg ttcctccgcg gctcacgggc aggagaccgg ttactttggt
    71161 ggccaggtct atcgtggaat ctgcaatgtc tgcattgttc agggttccgt ccaggatcgc
    71221 cctggaatcg atggatccgg tcccaaggtc ctgattgagt atagtggagt ccaggatggt
    71281 ccttgaatca atggccccgg tattaatatc ctggttgatc agggtggann nnnnnnnnnn
    71341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    71401 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg atggtccttg aatcaatggc cccggtatta
    71461 atatcctggt tgatcagggt ggaatccagt acggtccggg agtctacggc ccccgttttg
    71521 atatcctggt tgatcagggt ggaatccagt acggttcggg agtctacggc ccccgttttg
    71581 atatcctggt tgatcagggt ggagtccagt acggtccggg agtccacggc gccggtattg
    71641 atatcctggt tgagcagggt ggagtccagt acggtccggg agtccacggc agcggtgtgg
    71701 atgtcctggt taagtatggc tgaatccaaa attttatacg tagtgatggc attgtcggcg
    71761 atatcgtccg tgtaaaagga atagggcacc gacataaact gttggttgct caccagccgg
    71821 tagttgccgt cgtttttgat ggccagttcc acctttaaaa actggtcggc atcgatccag
    71881 gggatgtcca gcatccgggg atagggattg ctgccgctga ccgttccttt gccgatgatg
    71941 agggtgaaca ttccgtattt atccgtagtg gtggtatgcg tttcttccca ttgaacaggg
    72001 ccattcagcc ctttggtaat ggtaaaccgc accccaatgg cttttttgta cacgggtttt
    72061 cccgaaatat cggttccgac aatttctttg ccggtttcat ccacggccac cgcctggtaa
    72121 tagatcccgc tcagatctcc tattttattt tgggaaagag catggggcag gattcctatc
    72181 gaaaagaaaa tacttatata aaaaagtttg ttcattttat atcgttttag gttttaagct
    72241 atattttatt ccgaaaaaga aatggacacg ccgacgctta agccgtagcc aaaatttttc
    72301 gttttttgcc ccggatcctt ttctatattg gtcaggccca gcaaataatt taattccagg
    72361 taagtagaaa tataatcgga cagtttgatg ttcaggccag gggaaaagat taacccgtaa
    72421 tcgatattgc tcaggaggtt ggattccgtg atgttgaggt tttcattatg cgtcacctgg
    72481 aggccccgga gtaaaaaggc gtaatagccg gaggcgagaa aatagggatt caccgtttca
    72541 aaccgggtca tgtagcccag cccgagtttt aaatcggcat attgaagttt ccaggaataa
    72601 ttctggtcgt catacaccag gtttgcccct ccgttgcgca tgccgatccc cggtttaatg
    72661 atcaatccga tgtcattcac ataacggtag ccgatgccat aggccccggt gtataagccc
    72721 tggtattcct tgttgtggga tacgcccgcc cggtccttga atttgaagga agtaaaaagt
    72781 tgggaggctt ccaggaccag cccagattgt gcgggcagga ccgtcgaaaa ggctacaaag
    72841 aggcagagca ataaaaagag tttcattgtt ttcataatgc attttaattg gaaatgatca
    72901 atttcaaggt tttcgaattt tgtttggaat catgaatggt gattaaatac atcccgtttg
    72961 cccaggaggc gcaatggatg gtatgcgacg aaaggttggg gactttttcg gaaaggatga
    73021 accggccgtt tacatcggac accgtaatga cggagggccc tgtttcctct tcactggcta
    73081 aggtcaccga acggctggcc gggtttggaa atacgactac gttcaaatta ttcaagaagg
    73141 gattgctgat ttccttcacc gtaaaaagta cgggttgttg aaaaccctgt gcaaccgtta
    73201 ccccaatgga acccaactca gtatgaaaac tttgaccggc cgtctggccg atcaggatat
    73261 tttcagtgag ccctgaagaa ccataacttg aaatcgattg ccgtttgatg gactgggtcc
    73321 aggcggtacc cggcaggatg aatgccatga caaacagtcc agccatggac cattttttca
    73381 ttacaagaat ttgattcata aaattgatat ttatagaaag tgcctctcac ctggaatccc
    73441 gtgcaaatcc ccgcaccccc tatttattgt tttaactaaa ctctaagtcg ctcccgatta
    73501 ttatgttttt gataaagaaa ttttcacaaa tccttaagct attgagatta agccgtttcc
    73561 tcaagggggg ggccgggttt aaattccaat ggatcaagtg aattgttatt aaaattatca
    73621 agcccgtggc ttcaataatc caggagcagg tcatccgttg aaaattttgt tttatttgca
    73681 gaaggacctg ccattggcca aatcatggga aagacgaata taatcattta atttacaaac
    73741 aaatatatat attgagcgga acgatatacg gatggaatgt tcccccttgt tttaaattca
    73801 cccgctttcg gccagtttca gtgtcgggac ccggagacca aggaggacca tccctcaatt
    73861 cagcgatgac caactgagaa acaacagaat cagacgaagt acgagaactg gagggccaaa
    73921 gggcagtgca aaaccatagt agaataataa ttcaaccggg acaatggcca aagacagaac
    73981 ggcgcttcct ttgtcaccat tccaaagtcc atagcccgca acacattcga ccacgcaaat
    74041 gaggagaaat ccactgagga gcggcagggt cgtatggatg cccattcttt caaaaggccc
    74101 atacccgtag gacggaaatc caagcacata tggtattccc ttgcctttca tcaggctcca
    74161 aatcccatac aaacacggaa ttccaaatcc caagcctgca aaccataaac ataccgctgc
    74221 tgttttgatc actttattat tggctattga ttcattgaaa ttattgaaaa attaaacttc
    74281 caccgtattt tccagggtaa aacggagttc cgcgaacttt atgcgtctcc cggttttaca
    74341 accggtttgc gggcatttat tccggtcaat cgaaggagga tattcttatc ttattgatat
    74401 ctaagatatt atataaaaaa ggtatccgtt taggaattgc cttcccaaac cggaataaca
    74461 tatcagttac attcaggatg accagggcgg gtaagtccgg gatccggaac cttccgttta
    74521 acccaaatca tgcaatagag tttcgtaatg aaagccggaa ggccaataaa gactggcgct
    74581 cccggactat ttttaccgca aacataggca ctcctatggt gaggataaaa atgggaggcc
    74641 gaccttcata ctaaatcaac ctgttcggcc gggaagtgcc gtta
//
LOCUS       DAOQXT010000008        74000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_350_length_74000_cov_7.995510, whole genome shotgun sequence.
ACCESSION   DAOQXT010000008 DAOQXT010000000
VERSION     DAOQXT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 74000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74000
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_350_length_74000_cov_7.995510"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PKM27_02050"
     CDS             complement(<1..867)
                     /locus_tag="PKM27_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNR06066.1"
                     /translation="MKKYAYTLFIVFAVIVAYFFPQLFIEVNGVKLTTLVLPMLQVIM
                     FGMGTTMTTEDFMGIIKNPKGVFIGLVCQFSIMPFVGLALTLIFKFPPEIAAGIILIG
                     SSPSGLASNVMAYIAKANVPLSITITSCATLLAPFLTPFFMKTLGGGYIEIDFWKMMW
                     DITKIVILPVALGFLINTYLKVVAQRLQKVLPLLSMAGIAYVIVVVTAAGHDSLVTVG
                     GMLLIAVLLHNLSGFFLGYNLARLFKLPVQDCRTVAIEVGLQNGGLASALAIQMGKGA
                     TVGLAAALFGPLM"
     gene            complement(932..1726)
                     /locus_tag="PKM27_02055"
     CDS             complement(932..1726)
                     /locus_tag="PKM27_02055"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNR06067.1"
                     /translation="MSKSDQPKRPSSIPDTPEEVRLNKYVASCGIASRRAADELIRAG
                     KVWVNGKAVSEPGLRIGPKDRVTYQGKLLKPESNLVYLLMNKPKDTITTASDEKGRLT
                     VLDLVRKKVKERVYPVGRLDRMTTGLLLLTNDGDLARMLSHPSSEVRKIYHVVLDKPL
                     DPEELLRIKKGILLEDGRAEVDGISYIDGAGPNEAGITLHSGKNRIVRRIFEHLGYKI
                     IKLDRVAYAGLNKKDLPRGHFRHLSPREVTLLKNFGNRKKNKQVKG"
     gene            complement(1767..4298)
                     /gene="bamA"
                     /locus_tag="PKM27_02060"
     CDS             complement(1767..4298)
                     /gene="bamA"
                     /locus_tag="PKM27_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNR06068.1"
                     /translation="MKWMWKGLLLILGLVLTTGLRAQQDTVLPFIDYDLPKAYEVGQI
                     SVTGTFNSDANALIGVTGLKSGSKINIPGPEIQKAIRALWKLKLFTQVEIIQARVTDN
                     IVDLEIRLLERPRLARYSYTGIKKLYHEDLNEKLKRFINRNDVVTENKKQTAVNEIKQ
                     FFKDKGFYDVDVKVLEKRDTKMRNGLVLEFDVSPNDRVKIDQIIFEGNQLVSDKKLRG
                     KLSETKQKGKIFGKSKLVKEDLEKDKVKVIQYYNTVGFRDARFVRDSIWRNEDGTLNL
                     KLVVEEGPRFYIGDITWKGNSIHDEKQLNAYLGINKGDVYNQELMDQRLKYSQDGRDV
                     SSLYMDDGYLFFRVEPIEVSIENDSIDFEMRIYEGPQATIDKVNILGNDRTHEHVIRR
                     ELRTRPGEKFSRSDIIRSQRQIINLGYFDQEKVGIDTDVNPQRGTVDITYSVVEKPSD
                     QLELSAGWGGFQGVIGTLGVTFNNFAMRNLFNKKQWDPLPTGDGQKLSLRGQSNGKFY
                     QSYNFSFTEPWLGGKKANSFTLGAFLTKFNQELFGLGKLQIARGYIGLGSQLKWPDDN
                     FVTNTTLNIENIRLDGSLGSDRSFIDPRTGQPIIGGSYNNFSIKQTFARSTIYDPLFP
                     RSGSNISLTVSLTPPYSLFRKNLNTDGTPQEVFKYVEYHKWRLDAQWYSTLAGKLVLK
                     MQAKMGLLGFYNKKIGLVPFERFELGGDGLSNQNYGITGRDILALRGYEVADLAANAA
                     TSGGATIFNKFTMELRYPLTLNPNSSIFLLTFLEGGNAWIGSKNYNPFDLKRSAGLGM
                     RVFLPMFGLLGFDYGFGFDKDLPENDKKRFGSYGKFSIILGFEPD"
     gene            complement(4375..5136)
                     /locus_tag="PKM27_02065"
     CDS             complement(4375..5136)
                     /locus_tag="PKM27_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNR06069.1"
                     /translation="MDLKSGIDVSRLPQHVAIIMDGNGRWAKGQGMPRIYGHHNGVKA
                     VRACTEAAAEIGIRYLTLYAFSTENWNRPEQEVNALMALLVETIGQEVKTLNENNIRL
                     EAIGDISALPAASLQALKKGMRDTAQNTGMTLILALNYSSRWEITRATRQIASRVKQG
                     LLHPDQIDEHTVQSELETHNYPDPDLLIRSSGEQRLSNFLLWQCAYTEFYFTPVFWPD
                     IQKKDFWQAILEYQQRERRYGMTSDQLMMDHTPNI"
     gene            5299..5781
                     /locus_tag="PKM27_02070"
     CDS             5299..5781
                     /locus_tag="PKM27_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06070.1"
                     /translation="MKYLIKSGIGLILAWCLYPACNPVSCPKTKKELISHFNQFVSST
                     VRDKEFYSIREWEAKDKEFSGYLQDCYPLLDTTFYLDDKQRFWSDALRYYFKRYDNRV
                     TTELLNNKNPNSVILQTQIRSNWANPDEAFTRIFQEMTGLKFDEALKAARDSIAEEDQ
                     "
     gene            5792..6844
                     /locus_tag="PKM27_02075"
     CDS             5792..6844
                     /locus_tag="PKM27_02075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNR06071.1"
                     /translation="MYDVILSFITAFSLTYLVIPSIIRVAKSKHIYDEPDEFRRSHDA
                     PVPRLGGIAIFAGVVLSVTLWTPFQVFAGLQYILCALVIIFLVGFHDDIQPVSAVYKM
                     IGQVLAAFILVFKADIKLTSLYGIFGIYQLPELASIALSIFTIIVIINAFNLIDGING
                     LSGSISALISLTLGIWYLLINRLEIAVLAFSLAGAVIAFLKYNFTPARIFMGDTGSLL
                     IGLVCSTLTIKFIELHRELTDSAYSFKAVPAVAIGILILPLFDTLRVFTMRILRGRSP
                     MSPDRNHIHHLLIDSGLNHMQATGILVAVNLLFIFMVWSLQEIGTLNLLLVILAFATL
                     LSGMLFLIARKRRMGR"
     gene            7115..7978
                     /locus_tag="PKM27_02080"
     CDS             7115..7978
                     /locus_tag="PKM27_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HNR06072.1"
                     /translation="MAVAISTSGFIRFDGKRYVGDCETTSDVLGPFYRPDSPLRNNFV
                     IQGEKGEPIELSGKIKHEDCTTPYQNAKVELWHCDHNGLYDNESPDFKYRGTVYSDEE
                     GNYSFKTILPVPYGEGNNYRPAHFHLMISAEGYQPLVTQLYFVGDPWIGKDEMASSPT
                     AKRRILEVKNLKDGSKKVAFDISMSKKLAADPAIFHRLEGIYIEEKEAGKAKELFKKD
                     NQLWFKSNGMPFGQNLEYVSNNTFIIPGIPPKYNFSFVFEFMPSGAVKMMEVFQDEKG
                     EKQVIVSIKKQ"
     gene            8185..9405
                     /locus_tag="PKM27_02085"
     CDS             8185..9405
                     /locus_tag="PKM27_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06073.1"
                     /translation="MIRSVCFAVLTILFFVFPAAAQYGLRCDGTRYLQPVFDSISVST
                     VQFGKNKGPAGDSITLRMDVYQPAGDPAAARPAIVLAFGGVFITGDRTQLRPLALEFA
                     RRGYVVSSIDYRIWPFFVLGFPDSADIVDISVKAMGDMKAAVRYMKAAADQYRIDTSL
                     VFSGGVSSGSITALISAYLDPADSVQPYLKEVIEQNGGLEGSSNPATLSFSSRVRGVL
                     NLSGAMLDLSWLDEGEPILASIHGTADDFVPYAYGLAAGLVHLYGSSLIHPALEDKKI
                     KNQLISVPGGGHSNIYDEPRFLPYTDSFIQVAARLFHPIVCGEVSTRLREQQVQTAGF
                     YPNPATDHIQLILKDNRPPQYLRITDAMGRSVEFIGGGSRIDFLPGRLSPGLYVLELI
                     YEGNRRERGKLMIK"
     gene            9455..9988
                     /locus_tag="PKM27_02090"
     CDS             9455..9988
                     /locus_tag="PKM27_02090"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR06074.1"
                     /translation="MNSLEIEIERVRIDDLDLLQTIGRQTFYDTFSPANTEENMNKYM
                     DEGFSSEKLAAEINNKDSEFYFAKLEGKVIGYLKINSGSSQTDLKDHKALEIERIYVL
                     KEFQGKKVGPLLFDKALQIAKRKKVEYVWLGVWEENHRAINFYKKKGFMEFDKHIFKL
                     GEDEQTDLMMKLELKNR"
     gene            complement(10068..11201)
                     /locus_tag="PKM27_02095"
     CDS             complement(10068..11201)
                     /locus_tag="PKM27_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06075.1"
                     /translation="MSVRLVEINPSNRSDLKKFIGYPFELYRKHPVWVPPLWGDELDI
                     FSPVHNPVLKYCKLRLLLAYRDGRAVGRIAGIINQRENQLYHKAKARFGWFDFEDDEE
                     VSRTLLREIEDWSKARGMTELEGPMSFTNLDKAGLLVEGFEELPTSATWYNYPYYAKH
                     LEKLAYVKAADWIEHELDCPTEVSERFIRFNELLKEKYKIHSVEIRSRADIKKYALEV
                     FELINKTHNALHGFVAFEPEQVNLYVRKYSKILRPEFLSLVADQNNRLIAFGISLPSI
                     SRALQKSGGRLFPTGWYHLLQALKQNDRLDLYLIGTDPAWRNKGVHATIFYEITRSAV
                     KFGIKKVESNPELESNVQVQLLWKGYEYRQHKRRRTYKKELTP"
     gene            complement(11204..12163)
                     /locus_tag="PKM27_02100"
     CDS             complement(11204..12163)
                     /locus_tag="PKM27_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNR06076.1"
                     /translation="MLYVEQLLTLFEAYRKDHPFRGPVPELYEPARYLMELGGKRLRP
                     VFTLAVHQLFRNQLNEALPAALAIEVFHNFSLMHDDIMDQSPLRRGKPTVHSKYNINA
                     AILSGDWMLIHTYEILNQYKSKLAQSIIRLMNETAIKICEGQQRDINFETRTTIKKEE
                     YLDMIRDKTAVLLAAAARMGAMTAGASEEDQKHLYHMGLNAGLAFQLMDDYLDAFGDQ
                     TGKMNGGDILQNKKTLLFIKTLEKANDEDRKALLDLYEGREEDKVPKVLELFTRYGAG
                     DKLKALSQEYNQKASDALAAVEASSSRKEILNSLLTELLKRNI"
     gene            complement(12368..12517)
                     /locus_tag="PKM27_02105"
     CDS             complement(12368..12517)
                     /locus_tag="PKM27_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06077.1"
                     /translation="MQQLRLQGMYLAYRSQLELPMDHQLEGHDLIAHLLQSEELNRAN
                     EKTAY"
     gene            complement(12572..13009)
                     /locus_tag="PKM27_02110"
     CDS             complement(12572..13009)
                     /locus_tag="PKM27_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06078.1"
                     /translation="MEIYLDQERIALHLRKVRNKAYTTLRDHMPPHHQRMEQIKGWNR
                     DDILEQALRIGSSAYQAARLMMDNCIYIEQNYKACFGMIVLQKKYGSDRLEAPCKRSL
                     QGSRVNYTLIKNILERGLDRHATQISPSVFFDHDNIRGKEHYP"
     gene            complement(13031..13282)
                     /locus_tag="PKM27_02115"
     CDS             complement(13031..13282)
                     /locus_tag="PKM27_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06079.1"
                     /translation="MKDLNKALFEQLELLNEKPYKNTPYSRRYFFDQQEKLLLKPLPA
                     LPFLPKKAVRLTVQRNYHVQLTEDHHYYSVPYRYVGKKV"
     gene            complement(13279..14109)
                     /gene="istA"
                     /locus_tag="PKM27_02120"
     CDS             complement(13279..14109)
                     /gene="istA"
                     /locus_tag="PKM27_02120"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNR06080.1"
                     /translation="MAQKPIPMEKLKQVFQLHRDGIPIREIARRVGISRNSVRQYLSL
                     LSDCQGPDDVELASKVYSNDLLEMEAELMRQVTVHFSTTGHELTKTGVTRQLSWREYL
                     DTHPDGYSYSRYCFHLKQYLKNRDLSMHLEYSPGDMTMIDFAGKKLYYTDLETGELIK
                     CEVFISILPFSGLIYCQAVHTQQTADFTHLTNSMTKFYGGVTSTIICDNMRTAVKRSD
                     RYEPEFADICLQLSEHYGTTFSATRPYSPRDKAMVERAVNILCTIIFMGHYATEILPA
                     "
     gene            15155..15709
                     /locus_tag="PKM27_02125"
     CDS             15155..15709
                     /locus_tag="PKM27_02125"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNR06081.1"
                     /translation="MMQNFILILGLLLGCIFKLNAQSAPLKDRVFKDDSISLNLDKRP
                     RTALFLGLLLPGAGQAYNGRWWKIPLAYGAYGFSFYMISSTRKTYRVWNNNFNQAVRT
                     GKKVEVAPGIEFDSRQIKLIRDQARDNKDRAVFLMIGIHGLSALEAFVDAHLKNFNID
                     DDLGVHFYSPSVPGLSLCFTYNLH"
     gene            15804..16868
                     /locus_tag="PKM27_02130"
     CDS             15804..16868
                     /locus_tag="PKM27_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06082.1"
                     /translation="MNYLGRKCNYSFDYKFLLTCFIVFLTLFACQKESDSIIKLEIKP
                     EASQSLETHYKLAQIFENKKIIEFEGYKEFQRVAKFCIDNIDLLTGKIENGEIIFESP
                     FRDFIMAEIEMNKIKSEEDWFVFKNKWSEKVTIRDHSIDPKYLMYSKNILCNLNGILK
                     IGNKYHKYFDDFYASSNELNLLVNYKETDRYKVSQNVTVYEIKNSCNIPRSGSRANDG
                     SISCVYTVNPSGCNKRRIQGFLDWESEVFDEPGLPTAYTFNSQSTTRYQYLSIVWLNR
                     DADTINATVSHRIDIVSPPFSDIFSGSDVRTNSKLAIAYANPLPFAHPFTVSQHTSTH
                     IVKRDECNTINLSCNNYSAN"
     gene            complement(17275..18429)
                     /locus_tag="PKM27_02135"
     CDS             complement(17275..18429)
                     /locus_tag="PKM27_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06083.1"
                     /translation="MKKVLFILFLMLPAGLAAQDSLEEKSGKFTAGGYLKELAYLTLD
                     EINQEEYITSILHHRLNMQWMPNERFSASVSFRNRLIWGDEVRLIPGYVALLRNKNEG
                     LDLQKAWISGKSMVLHTSVERLNVEYTGTRWNFRLGRQRINWGMTTTWNPNDIFNTYN
                     FLDVDYEERPGNDGARIQYFSGGNSNVEWAIAYNHFKDKPVTAIKYTFNRWKYDFQLI
                     GGWYFDRPSLGMGWAGNIRNLGFKGEAQFFFNKNNSPSNLNLSMEWNYLTDNNWFFNL
                     GILFNKQGKYKPIQNWAEIDLNLSAENLMPTRYNVIGTVSREFTPLFSGNAGILFAPG
                     TNLLILLPSLRYSLANNLDADLVWQSFFVEDQNRFSSMSHVAFVRLKLSY"
     gene            complement(18430..19134)
                     /locus_tag="PKM27_02140"
     CDS             complement(18430..19134)
                     /locus_tag="PKM27_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR06084.1"
                     /translation="MNQTVIDAHDVSKTYNPEAIPVPAVNHVHLHIEQGEFTALVGPS
                     GSGKTTLLNLIGGLDRPTGGKIFINGVDITQLSDNQLIDFRLHHIGFVFQSFNLIPVL
                     TAKENIEFILLLQGVDKTTREKRVLQLLKEIGLEDKMNVRPSQLSGGQQQRVAVARAL
                     ASKPQFILADEPTANLDSKAAANLLDIMYKLNQEENMTFIFSTHDKRVIDRARRVVTL
                     VDGRIDSDTAFKPLEH"
     gene            complement(19153..20355)
                     /locus_tag="PKM27_02145"
     CDS             complement(19153..20355)
                     /locus_tag="PKM27_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR06085.1"
                     /translation="MAWRNIWRNKMRSLIIMASVALGVFSGVAILGIYKGILKSRVRT
                     VIDQEVGHLQIHHPEFKKDFGAAYIIPGSDSLIQQLLGWTEIRALAARTVSTAMLSAA
                     TGTSGVQVTGIRPEEEEQVSHISRKIVEGSLFDPAKKNQILISRKLSDKLKLKLGNKI
                     VLTLTNKDNDIQAGAFRICGLFQTINTPLDERTVYLRQDELNDLLAIPGQSHEITLIL
                     QSDDQLDTIHQRLKSRFPALLTENWKEISPETNLMVVSLDEYMFIYIIIIMLALAFGI
                     INTMLMAVLERTREVGMMTALGLTKARLFGLILSETILLTLAGVPVGMGLAWILIWYF
                     ARHGINIAAVAKEAMSSFGFESWIFPEFPYDQLVMVLSIVAITALLSSILPSLKAVNM
                     RPSEALRR"
     gene            complement(20396..21613)
                     /locus_tag="PKM27_02150"
     CDS             complement(20396..21613)
                     /locus_tag="PKM27_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNR06086.1"
                     /translation="MWLIKLAWKNIWRNRNRTLISMSAIFFAVIFSVVANSLWKGTFG
                     ALIQNLVGYYSGYIQIQQAGYWDEQVLDNSLEWSDSLKAEISGENNIDALSPRLESFA
                     LMSSGDITKGCLVVGIDPMRESEVTQLQERLKKGRYLESGDQAILLTEGLAEQLQLSL
                     EDTVYIIGQGFHGATAAGKYPIKGILKYGAPDLNNRLAFLSLPAAQELYGAENRATSV
                     VISLHDQNLLEETVLGLKKRLDSGLVAMTWKDMMPEIVQHMKTDHFTMQIIQYLLYLL
                     IAFGIFGTLTMMMVERQFEMGMLIALGMKKGNMILLMVIESVFTVITGCLAGLAASIP
                     VAFYFKNNPIRFGGEFGKAYERFGFEAVMPGSTEPGIFWSQGLIVLCLGLLLSLYPLY
                     KILRMDPVKAMRR"
     gene            complement(21613..22260)
                     /locus_tag="PKM27_02155"
     CDS             complement(21613..22260)
                     /locus_tag="PKM27_02155"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNR06087.1"
                     /translation="MRGNTSQVEMNIKTIRPSWSREMDLRVWSKGNDFAMILIQSPAR
                     DRGITFLKRGKEVWNWIPTLEKTIKLPPSMMSQSWMGTDFTNDDLVRESSVIRDFEHS
                     LGTDTLILGRPCHTIWMVPKPEAAVVWGKLMVCIDKKDFMELHTRFYDEEGQLVNIMN
                     AYDPALLGGRLIPTRFEIIPVEKRNQKTEMRYKKVVYDQPIADGFFSINNMKNIR"
     assembly_gap    22356..22455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22493..23512)
                     /gene="add"
                     /locus_tag="PKM27_02160"
     CDS             complement(22493..23512)
                     /gene="add"
                     /locus_tag="PKM27_02160"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNR06088.1"
                     /translation="MESDHSSPAISLLPKVELHLHLDCSLSYHALHLLDPQITRDQYH
                     RQFMAPAKCSGLMELITPVFQSLKYMQTAEQLEVVVLDLFEQLKKDKVFYAEIRFAPL
                     LHTEKGLTPGEAVSIVEQATASAIKHYGIHARLILCTLRHFTEAQSMETVELVQTFKN
                     SHVTGFDLAADEAGYPVDQHRKAYDYAHQTGIPCTCHAGEARGADSVWECLRELRPSR
                     IGHGVRSIEDPALIQYLRDQHIHLEICPSSNVQMNLVETYGEHPVDKLYRLGVSLGIN
                     TDNRTLSDITLTREYEKLQSHFSWGIRHFLDCNLHAIRAAFIPEPLKQELAERIMAEY
                     KSMNV"
     gene            complement(23553..24554)
                     /locus_tag="PKM27_02165"
     CDS             complement(23553..24554)
                     /locus_tag="PKM27_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNR06089.1"
                     /translation="MAKTIFNYVKLGIFVIAGMAFLVILLYYIGKNRNIFHPSFLLKT
                     HFQHIQGLQPGNNVRYAGIQAGAVKSINILNDTLIEVVLDIDNKFKNIIRQNARVSIG
                     NDGFVGNKIVNIEPVKEEAALVEEDNVLASKEALDTDAALRTLSGTNQDIADIARELK
                     TLLTNINQNPALVKLVTSDEIPNDIRAALADIRRAAHQLQLSAAEVNTLLDSVSRGKG
                     SLGALFQDTLIYANLLNTSEKIKLAGEESNRITRQVSQVVQSLKDHIDQGKGPVQAIL
                     RDSSMTHSLQNSLENIEKGTSSFNQNMEALKTNFLFRGYFRRLDKKEKKDSLRAVRE"
     gene            complement(24572..25378)
                     /locus_tag="PKM27_02170"
     CDS             complement(24572..25378)
                     /locus_tag="PKM27_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR06090.1"
                     /translation="MADPDSNDTVISGVAPGISRSAPQSKEEVIVISHLYKSFGSNRV
                     LQDFNLTLYKGQNIVVLGKSGSGKSVLIKCIIGLLRPDAGEIFVFGKNVPELNHFALD
                     ELRARIGFLFQSNALYDSMTVRDNLEFPLRRHWIQVTQAQVNDMVMEALEDVGLAHTV
                     DMMPVELSGGMRKRIALARTLILKPDIILYDEPTTGLDPITGKEIIDLIVEIQNKYNT
                     ASLIISHDMNCVRLASDLVAVLIEGKCYALDTYDRLKSSTDPNVKPFFEQ"
     gene            complement(25371..26141)
                     /locus_tag="PKM27_02175"
     CDS             complement(25371..26141)
                     /locus_tag="PKM27_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR06091.1"
                     /translation="MATDTTLKKFLDETGRIAVFTGRFLNEWFRPRYEISEFFRQCYI
                     IGYKSLPLIGLTGFIIGLVMTMQLRPSMVNYGVESQIPAIVGISIIREIGPIITALIF
                     AGKIGSSIGAELGSMKVTEQIDAMEVSGTNPFKYLVVSRVLATTFMLPVLVLLSDCIA
                     LYGSYLGVNIKGITSWSLFWNKVFTNLIYADIFPAFIKTFFFGFIVGIIGCYKGYHSS
                     KGTEGVGRSANSSVVVSSVLIFITDLLVVQVTDLIGYG"
     gene            complement(26238..28400)
                     /locus_tag="PKM27_02180"
     CDS             complement(26238..28400)
                     /locus_tag="PKM27_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06092.1"
                     /translation="MYAVIWRNWNLVDPDRIAQTIGCTAEDIHSIAASMGLPPAQPIP
                     PGFRKQLYITVLRRNWHLLPYDQLLNLADMTPQELEFSLKEDDFLFHKFGNLKPACLP
                     LKYSAPNPQERAIAWEIKQLVGKKFKKSFKIPAEPLFGFIQDMRNRDLSVPIPVADLS
                     AAEGLRFIYSYFGVFGDPLIDDHSDPYPEGLLAKLASKGINGVWMHIVLNQLAPGGKE
                     FPEFGAGHQTRLANLKRISQQAKKYGIKISLYVNEPRAMPLAFFKNRSSLAGVQQGNY
                     MTMCTSNETVLNWITHSLAYVFKEVPDLGGIFTITASENLTHCASHDHQAGCPRCSKR
                     DYADIIAEVNQAMELGVHRANPDAKVIAWDWGWHGHGDAPDIIARLPKSVWLMSVSEW
                     AKPIERGGIVSQVNEYSMSAVGPGPRAQRHWALAKEAGLKTVAKVQFNNSWELSSVPW
                     IPALDLVAQHASNLSRVDVDGFMLSWTLGGYPSPNLEIAQRFSQDPGADPKAVLDSLA
                     ISRYGSQAAPFARKAWTSFSNAFLEFPYHQSVLYSGPQQYGPSNLLYPKPTHYLASMV
                     GFPYDDLNKWRGPYPADGFAKQFEKVAAGWKKGLDQFKKVLITVRRENKETALKDWGI
                     AKAAWLHFASSANQARFILLRDSLGKASLSSANKQMLKKQINHLLDEEIKMAVQLFEI
                     IRRDSRIGFEASNLYYYVPQDLMEKVINGEFVRKTLTH"
     gene            28704..28868
                     /locus_tag="PKM27_02185"
     CDS             28704..28868
                     /locus_tag="PKM27_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06093.1"
                     /translation="MSDEEKKIPPDEFNRSLSRLLNQIAECYRYLGPAQHFRANASGN
                     LIKGRAWLRE"
     gene            complement(28986..30521)
                     /locus_tag="PKM27_02190"
     CDS             complement(28986..30521)
                     /locus_tag="PKM27_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06094.1"
                     /translation="MKYLFRCLLMFLFVSHAPAVQAQIIETTYNWGSFDHSCWKVQPN
                     EYGYVMVGSKFFEPNNTSLYVQGFDHQGKSLFLKSHTASGFSRLDVFWKSFVLSHDKS
                     KIFAVSSGSQSGQKAYALLTDSEGRKIWDRVSDLPNGITFGGVTNAKNGGWVAVGSSN
                     FYDLAVVKFDPQGRIEWTKIIPISGFGWSIVASWDGGYFIGSTGSRITKIDHSGNLEW
                     STAVVLPPSPSGAYTYTEFEELFEYILPAGGISLPVGIILTGSCFSNSHSGAYTAMIN
                     HGGTVKWSMVHAPQNTALAGTPVSWISNAILEGPYVTTSWRTGPVSSGGTMHYARQVF
                     YNGAVAESGSLDNTIPVQEAFLIKSKDKYIVGGTRGGYSAAYSYINDQLSPWWLEGFR
                     RSEGENLPIAEASRITETLVRVNFNNTKPIYKYHPASRVFNSEMQVFPNPSTGLVKVG
                     GRIEHGALLRVTDITGRIVLEKQIRPGEAQVELDLKNQGKGLYNVEMIGSDHIISQKI
                     ILQ"
     gene            30948..31892
                     /locus_tag="PKM27_02195"
     CDS             30948..31892
                     /locus_tag="PKM27_02195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR06095.1"
                     /translation="MSILLNQIAECYRYLGPAQHFRANAYSAAAKTIGQMKTSVLALD
                     HDVKKLSQLKIIGESIAENILEYADTGKISRYEELKKIVPVDLLELMQVRGIGPSTIR
                     LLHEKLHINNKEELLKALEENKLSDLENFGPRKIENLKKALKIFKGDRRMPLAEAGRV
                     GKALLEYVLKIPQVERAELAGSLRRRKETVGDIDLVIQAEDRHRNAITNRIVAYPLVA
                     EVIARGPTRVSMVLNPGHVQVDIRVVNKDAFGSSLLYFTGSREHTLQLRRQAQKMGLK
                     LNEYGLFEVGSGKKLAGETEEGVYKALGLEYLEPEERV"
     gene            complement(31979..32818)
                     /locus_tag="PKM27_02200"
     CDS             complement(31979..32818)
                     /locus_tag="PKM27_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNR06096.1"
                     /translation="MKKILSAFDGLKYSVSTQDYSVDLAHKLNAHIVAVFLDDREYHG
                     YKIYDLITEDGVSEKLKKELDNQDRAHRKASAEKLDEVFKKGKLQFTIHHDKNNALKD
                     LLHESIYADLLVINGTETFKPRNEKAPTSFIRHLLEDVQCPVIVVPSKFKAIEQVVVL
                     YDGQTVSVFALKMFNYIFGKNDGSNVEILSISTGGQTMHTPDSRFIKEYLKRQLPKTK
                     YTVLKGYPDEQIVNHLKEKPAGTLVVLGAYRRGKVSRWFRESMADVLMSELKLPLFIA
                     HNK"
     gene            complement(32918..33274)
                     /locus_tag="PKM27_02205"
     CDS             complement(32918..33274)
                     /locus_tag="PKM27_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNR06097.1"
                     /translation="MLSATFIKSIALSFPETEQHPHFDLVSYRVNKKIFVTVNAPQKR
                     CTLKFDKEYQDLFTSVGRGAIYPVPNAWGRLGWTTAELEKLNPEILKDALLIAWRCVA
                     PKAFKKKYPQMYIDEE"
     gene            33398..34948
                     /locus_tag="PKM27_02210"
     CDS             33398..34948
                     /locus_tag="PKM27_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HNR06098.1"
                     /translation="MKKTTISTLFSLMMFAAFTQNRPAPGPPPPAPSAPAPKEEKKQS
                     TDIVFHPESSVVTQHEITIKGQRVPFKATAGTLPVWDEEGKTLAGLFYTYYERTDVRD
                     RDSRPIIVSFNGGPGSASVWMHIAYTGPVVLNIDDEGYPVQPYGFKDNPHSILDVADI
                     VYVDPVNTGYSRPVSKDIPRNRFFGVNADIKYLAEWIGTFMTRYQRWASPKYLIGESY
                     GTTRVSGLALELQESQWMYFNGVILVSPTNLGLVRSAPQTAALRLPYFAATAWYHKKL
                     PADLQQKDLTAMLPEVEHFTMNELLTAINKGSMLPDVERKNIAQKMARFSGIDAEVYL
                     QNNLDVSTSLFWKELLRDEGYTVGRLDSRYKGMDLKDAGERPDYNSELTSWLHAFTPP
                     INMYLREKLNYKTDLKYNMFGPVRPWDDSNNNTGMNLGKAMSSNPYLHVMVQSGYYDG
                     ACDYFSAQYNMWQIDPAGKFKDRVSWKGYRSGHMMYLRKEDLASSNEDIRQFLKMAGT
                     KPGQPAKY"
     gene            35088..37127
                     /locus_tag="PKM27_02215"
     CDS             35088..37127
                     /locus_tag="PKM27_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06099.1"
                     /translation="MNLSGTGRNVKRTIYYLTAVLCIAGIAFECSNQPAEIRDPLERN
                     LVLAAGNRPELEKVIDHFRKDPDSLKLKATLLLLSNMEDKFHYSGEWLRQADSLFFSR
                     EGGLGEEDLQLLRDSVYGLIGSPAHADIRKIQDLQHLTSSYLIQNIEDAFESWQKAPW
                     KEAVSFDAFCNYILPYKSYNEYPENWRPLLRARYNNLLLENGVPQAMEDICCALVDEE
                     KKWLKYSEEPRGYPGALSISSILKLQKGSCVEWSAYGAEAARAFGIPVAIDYVPQYGN
                     RYSTHKWNALVLSDTSFLSFLGAESRPGDHAPIREGETKFAKVFRWRLGADPESFAVK
                     ARKAGIKAIPPYLEDPRIKDVTAHYTLVSDLSFEVKGDPGMPLYLCVFKNWDWEAIAG
                     GLIDQDKVRFPQMGRAVLYMPMFYQNGRYRPAGPPVGLMADNKIQVYRPGGEGNLVLK
                     RKYPFKRTEEWLREHSLQNARFEGSHDPEFTKPALLYYVLRNVRPYRGARVNNLALKD
                     QWLYDSLWREAPVRHKDSFQYVRLLFKKGNEFRLGEIEFYADGKTRPLSGKILGNIPQ
                     APLAFDGFPGKCIKLPKDTSQARWVGLDLGKKTAVEKIRFIPADDDNFVEAGQTYELF
                     YWKDKWVSAGKQKALSDSLEYQKVPQGTVYLLRCWDCNNLEERPFTYEGGKQIWW"
     gene            37174..37620
                     /locus_tag="PKM27_02220"
     CDS             37174..37620
                     /locus_tag="PKM27_02220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR06100.1"
                     /translation="MAVYTITALNESDYPRILEIWESSVRATHHFLKEEDIQLFRKWI
                     PLHYLKAVELSGIRDEEGKLWGFSGIHGQSLEMLFIHADQRGKGLGRQLITQAIRTRQ
                     INKVDVNEDNPEAIGFYNKFGFRVVGRSELDGSEKPYPILHMERMK"
     gene            38251..39042
                     /locus_tag="PKM27_02225"
     CDS             38251..39042
                     /locus_tag="PKM27_02225"
                     /inference="COORDINATES: protein motif:HMM:NF023805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HNR06101.1"
                     /translation="MKLLHFITILLALFIISCQQDPQVRTTEEELTGITAEVKQKIIN
                     LGFNPENSFKMEGGYMVEGDIFLTEEELHSLPNNRIPVLEQYRTTNLVTVNGTKTISI
                     SVNKDVASFTQALDSAIARYNAENLTLKFKRVSSSGNIKISKAASSATYLANSGFPSS
                     GRPYSNISVNLTLLGAQPFGTIATVLAHEIGHCIGFRHTDYFNRSISCGGSALGESSS
                     GGAIHIPGTPTDATQAAQSWMLSCIGSGHDRPFNADDKVALDYLY"
     gene            complement(39172..41211)
                     /locus_tag="PKM27_02230"
     CDS             complement(39172..41211)
                     /locus_tag="PKM27_02230"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HNR06102.1"
                     /translation="MEHKHHHQPLHQETPPVHGNPNPPMGHAGHDHHSMMMDDFKKRA
                     WAVLLLTLPILLLSEMIQHWLGFHLVFAGSKGLLWLLSSVVFFYGGWPFLRGWLDEMK
                     SWQPGMMTLIGFAITVAYIYSSATVLGLQGMDFFWELATLILIMLLGHWIEMKSVAGA
                     SRELDLLVRLMPDDAHLVHGNHIMEVQTTSLKTNDIILVKPGEKVAADGLITEGETYV
                     DESMLTGESRPVAKKHGDQVIAGSINGNGAIKIRVSHGAKDSYLARVVKLVQDARQSK
                     SKTQLLADRAARWLTLIAIAAGLITFAVWYFSGNGLAFAMERMVTVIVICCPHALGLA
                     VPLVVAKSTALSAKHGLLIKNRTAFENARKISTLVFDKTGTLTLGRFEVSRVVPVLPE
                     LNEYELIRLAAALEQNSEHPIASGIMDKAKSMGIPVPSVQKFNAITGKGIEAHVEGKD
                     IKVVSPGYLKENQIPFPPDYTADLSETVVFVLGGDEVSGFISLSDTLRPESAGAIRDL
                     RRENIKPVLLTGDRLEVAAAIAQTLNMEHYFAEVLPHQKLDKIKELQQEGEFVAMTGD
                     GVNDAPALAQAQVGIAVGSGSDIAAETADIILVNSNPADVVSLIRFGKATYRKMIQNL
                     VWATGYNIVALPLAAGILYPQGFLLSPAAGAVLMSLSTVIVAINAGMLKIKGRQP"
     gene            complement(41215..41772)
                     /locus_tag="PKM27_02235"
     CDS             complement(41215..41772)
                     /locus_tag="PKM27_02235"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNR06103.1"
                     /translation="MIKFQQIFVFCCLSGIGWGQSGEINYNPNQKSDTTRKSIPARAI
                     LRLEQDSITIDYHSPGVRRRIIWGGLVPFGEVWVTGAHNATKLSIPFDFVMGGKRLPA
                     GEYAIFSIPGKKEWTVILNKHWEQHLTSEYDPKDDLIRIRVKPRKTEHTERLQYFMET
                     HQDGKIDIAIVWEKIRIPFNLTIQR"
     gene            complement(41789..42985)
                     /locus_tag="PKM27_02240"
     CDS             complement(41789..42985)
                     /locus_tag="PKM27_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR06104.1"
                     /translation="MKSLIFSVILLFLLACNQKTGPVKDPELYYTCSMDPQVVEYKPG
                     KCPICKMELTPVKISKGNRRDELELSDQQILLGNIQTDTLATSRIGKKILLNATLNFD
                     QRKINSVSSRIMGRIEKLYYKNLGDYVVSGKPLYELYSEELNTARLEYLQALENKNTY
                     PKDGQINMDHLIQNAKNKLILWGMSERQISEWTILSNPLATTFLSPSSGYITELNVLE
                     GDYAMAGSLIVKLADLSTLWAEAQIYSSQMADVNLKSKALVRFPDLYNRDVSGTIEFM
                     TPELKADSRILLLRVSIPNPDHTLKPGMQAQILLTSPLDQVLTLPLEAVLKDSRGSTV
                     WVRSGKNKFRPRMVQEGRESEGNLEILSGIAPGEEVVTRGAYLLNSEYLFKKGADPMA
                     SHTHDQ"
     gene            complement(42982..44106)
                     /locus_tag="PKM27_02245"
     CDS             complement(42982..44106)
                     /locus_tag="PKM27_02245"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR06105.1"
                     /translation="MHPEIMEDEPGSCPICNMKLVQKNQTEQDPSIPRLEDLLKPTHE
                     FIISTVRDTTLLKKEVSLIGEATGVIDYDTREVYRISSNTEGRIEKLYIRYRFQKIHE
                     GQRIMDVYSPELVTAQQNLLFNLRQDPDNESLVRAAKQKLILLGMQTSQMEEVIKSGE
                     PSLTVGVYSKRNGYVIETERMEGRSEGAMANKEGEEEPLTLKEGMYVQKGQPVLSVYD
                     PKRTWAIINIHGENQGWIKIGNEAILRAETSPQTILRGKINFIEPFYQKGSKTLTARI
                     YLDHRYGQLPIGSRITAKITGPSKTSYWLPKSAVLSSGLQKVVFLKKEGGFKAIPVQT
                     GLVMEDLVEFKDGLSIHDRFAINAQYLLDSESFITVKNKP"
     gene            complement(44334..45590)
                     /locus_tag="PKM27_02250"
     CDS             complement(44334..45590)
                     /locus_tag="PKM27_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR06106.1"
                     /translation="MKTRLTLIPVLSFLFLRALSAQEIYTLDRILDSIRQNHPVVKMY
                     TREIRSMDEAAEGARSWMPPQFAIGSFMTPYNINRWSREGEMTGMGSIMISGEQMLPN
                     KKKLDAEERYMKAMSSAEYDQRNNVLHELVHDAKSLFFETMILNKKLAVLGSNRQILE
                     FMVQNAESRYQFGLGKIGAYYKAKAALGNLKNMQWMIESEIRDKHFRINTLMGRNPTI
                     PLELDTLFHLNDFSLWVFDQALFFSNRSDIRALDQRIRLNWLRQDAEKQTLRPQFGLR
                     YEHMFGFGGQPLLFNLMGMVSIPMVKWSSKMTRANIESLKWQAMSYEAKKEMLANEYS
                     GMAYAMRNELQLKIRQLSGYEETIIPALKNNLQTMQLGYEQNTEELFMLYDAWEALNM
                     KQLEYFDLLNQALRSQVELERLIQQK"
     gene            complement(45625..47514)
                     /locus_tag="PKM27_02255"
     CDS             complement(45625..47514)
                     /locus_tag="PKM27_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR06107.1"
                     /translation="MLDPKERMRRVEESSKLIAKAVVSSILVTLVSFSPILFLSGQEH
                     KLFSPLVWTKTFAMAGSALVAVFLVPVLMVVLLKGKVYSENKHPISRFIISLYSPVIR
                     WCLKWKKLTLFFCIALVAGSIPYLLRLGREFMPPLDEGSLLFMPVTLPDISNAEIKRI
                     MQVQDKLMLTVPEVQNVLGKAGRASTATDNAPISMIETIVLLKPQALWRKGITKNDII
                     NELNAKLQIPGVINGWTQPIINRINMLSTGIRTDVGIKIFGQHLDSINQLAQLFKKTL
                     EGLDGVKDLYVEPIIGGKYLDIRIKREELGKYGLTIDDVNMFIESALGGMTLSQTVEG
                     RQRFSINLRLGQDFRNSIEKMKQIPVMTMDFGPVPLGTLADIELTEGPPMIASENAML
                     RGTLLFNVRGRDLGSTVDEAKNKLAEAMNKLPKGYFLQWSGQYENQIRAEQRLKLIMP
                     VVLAIICIILYFTFRSFREVLVVLSAIPVALVGGVYSLYYFDINFSVAVAVGFIALFG
                     IAVETGVLMLVYLNHSWEKKIKTFREQNLKLNPHALEEAIYDGAVLRVRPKLMTVIVD
                     ILGLLPVLLATGTGSDVMKPIAYPFVFGLITSTLFVLIVLPVLYALIRNADLKKDLQE
                     PEDLT"
     gene            complement(47520..48785)
                     /locus_tag="PKM27_02260"
     CDS             complement(47520..48785)
                     /locus_tag="PKM27_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR06108.1"
                     /translation="MIDKLIEGALRNRIIVLLVAAIIAAYGFYSVRTTPVDAIPDLSE
                     NQVIIFTEWMGRGPQIMEDQVTYPLVSNLQGLPKVKNIRANSMFGMSFVFVIFEDQVD
                     IYWARTRVLERLNSAQQLLPKGVVPTLGPDGTGLGHVYWYTLDAPGHDLGELRALQDW
                     YIRFALQTVEGVSEVASFGGFQKQYQVVVNPLKLQYYRIPIMDVVRALEANNKDVGGR
                     KFEQADMGYIIRGLGYIQNIKQVEDIAVTHINSIPVRIKDLADVQMGGELRLGIVEEN
                     GEGEKVGGIVVMRYGENANDVIARLKSRMQEVEKGLPQGIRFKMAYDRSELIQATIAT
                     LKEAVLEEILVVSLVLFVFLLHLRSALIVVITIPMSVLIAFILMKWFGITSNIMSLAG
                     VALAVGDLVDAGIVMVENTMKSLSGELEQ"
     gene            complement(48878..49291)
                     /locus_tag="PKM27_02265"
     CDS             complement(48878..49291)
                     /locus_tag="PKM27_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06109.1"
                     /translation="MKKTSNYLHSVLFCASSLLLVIQSCSSTQKGEVTAKIASSSKEY
                     SVINTGESILLYKYMHKAHSAKEVDKYAPKYYFTTASSDVLKDLTKENLKKAFPTNHA
                     FHNALDAHFNSDAELINYDTFHKMYKLNWLLKNNP"
     gene            complement(49322..51595)
                     /locus_tag="PKM27_02270"
     CDS             complement(49322..51595)
                     /locus_tag="PKM27_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNR06110.1"
                     /translation="MKFQCSVHGLRSSIFHLYFGKIGLPLISFLLPVLGFAAGAKIVR
                     YDLFITDTLVNYSGKTRKAIAVNGTIPMPTLEFTEGDSAVIYVHNRLKEETSIHWHGL
                     ILPNQYDGVPYLTTRPILPGQSHLYTFRLIQNGTYWYHSHTKLQEQSGMYGAFIIRKK
                     SGDPTWREEDQIPDYALVLSDWSDEKPFEVHRKLHNATDWYAIKKGATQSYAEAIREG
                     HFKTKLVNEWKRMLAMDVSDVYYDRFLSNGQAELEAAEFKAGDKIRLRIINGSSSTYF
                     WLNYAGGKITIIANDGPDVVPVEVDRMIIAVAETYDVVVTVPESGLAHEFLATSEDRV
                     KSTSLWLGKGTKQPATPLPRLKYFEGMKMMNEMMKMNGDLDDMGMNMSLQQMDMNVVM
                     YPEITGPGKDRKKQEHQHGQPEQNQNDATPEIVTLNYAQLRSPFKTTLPQAPVKTMRF
                     ELSGNMNRYVWSLDNKTVSETDKVLIRKGENIRIILYNGSMMRHPMHLHGHFFRLLNG
                     QGDYAPLKNVVDIFPMETDTIEFAATETNDWFFHCHILYHMMSGMGRIFSYEDSPPNP
                     EIPDPAASLKKLYRDDREFHFMFQNDFASNGNDGEMMLQNTRWSLGAEWRLGYHDDHG
                     YETETHIGRYLGKMQWLMPYLGFDWRYRKSDEREKNWFGQVNTKDQRGALCLGLRYTL
                     PLLFKADARVDMDGRLRLQLMREDIPLSKRFRMALMYNSDREYMAGLQYILTKNLALS
                     THYDKDMGWGAGITMTY"
     gene            complement(51635..52147)
                     /locus_tag="PKM27_02275"
     CDS             complement(51635..52147)
                     /locus_tag="PKM27_02275"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HNR06111.1"
                     /translation="MKTINPILILAVIFIGTAFRSRAQAGSELFKDLFQSYFQIKDAL
                     VKSEAENASLHGGQLVAALDKMEMNKLGHEEHLAWMKVEKELKQDAKDLAGTKKITRQ
                     REIFIRLSENIYSLIKSSKPLEAVYYQHCPMANGGKGAHWLSKENPVKNPYYGASMLS
                     CGRTVETIQQ"
     gene            complement(52227..52628)
                     /locus_tag="PKM27_02280"
     CDS             complement(52227..52628)
                     /locus_tag="PKM27_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06112.1"
                     /translation="MKKLLSSFLLILYLGTSSGLTLHTHYCMGRLAGWSLGYELPDIC
                     GKCGMNKSEGKDKGCCRDEHHVLKTSVDQKTLGSSFSLNLALAETVLPALHPEPVYLH
                     FQDRALPAPVNAVPPPLNTVAVYIRNCVFRI"
     gene            complement(52757..53473)
                     /locus_tag="PKM27_02285"
     CDS             complement(52757..53473)
                     /locus_tag="PKM27_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNR06113.1"
                     /translation="MTQTYTVTGMTCGSCELAVKNALQSLPHVKSAEVSKTAQQAKLE
                     LEKAIPLSELQQALGPNKYKITPHPSAHEATPEKSWIQTYKPILLVFANLLGLTLFIE
                     AYRGSFGWMRWMNHFMAGFFLVFSFFKLLDLKGFAESYARYDILAKRWAPWGYVYAFV
                     ELGLGLLYLGGWFPLVTNTITFAVMSLSLVGVLQTVLNKRVIQCACLGKVFDLPMSTV
                     TIAEDALMIGMSLIMLLYMI"
     assembly_gap    53827..53926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(54370..54942)
                     /locus_tag="PKM27_02290"
     CDS             complement(54370..54942)
                     /locus_tag="PKM27_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR06114.1"
                     /translation="MRLYIKNMVCNRCVMAVNQELEKLGLHPASISLGEVEWSHEPTA
                     QQLQNLGQRLRALGFEVLDNQKQKQIEKIKSLLIGLVQQAAIEEHFSLSEFLGKALHK
                     DYSYVSRLFSGVEGITIEQFFILQKIEKAKELLVYDELSLSEISWKLGYSSVAHLSSQ
                     FKKFTGLTPTAFKKIGGLRKSIDKVIVSGQ"
     gene            complement(54951..56180)
                     /locus_tag="PKM27_02295"
     CDS             complement(54951..56180)
                     /locus_tag="PKM27_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06115.1"
                     /translation="MMTRPLLGFLVFWTYWLPAHAQIDPDLLKIPGDSSKQMLNMDAV
                     YNRPFTSAGKLPVSLGGYLEANWQHLGTDGISEGHQFQFRRMTLFVSSTIARRLKFLS
                     ELEFEDGTKEIALEFAALDLEWFPLLNLRGGVILNPIGAFNQNHDGPKWEFIDRPISA
                     TQLLPATWSNAGFGLYGKYNKRDWMFGYEAYLSGNFDQSIIDNGLNRTSLAEAKKNAD
                     RFEDIHSGLPLFTLKTAARHNQLGEVGLSYMGGVYNKYQEDGLPLDEKRRVDVFAIDF
                     NGTLPVLNTFITGEWAWIRVNVPPTYTEQYGNKQRGGFVDLVQPLVKKRILEWEDAVM
                     NLAVRLEYVDWNRNKFVSTGDPIADHLWSLVPGLSFRPSGQTVIRLNYRIQRQWDLLG
                     NPPARTGGFSFGFSSYF"
     gene            complement(56177..56611)
                     /locus_tag="PKM27_02300"
     CDS             complement(56177..56611)
                     /locus_tag="PKM27_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNR06116.1"
                     /translation="MDRNTFIKTCGLACLAGLGGLSACGSIRRVRGDIDADDLVLPLD
                     RFESGKEGKTVFRDSVIVYHEILKYPVCVFRVSGQEYRALWMRCTHQGTELQVFGDRL
                     QCPAHGSEFDSSGRIRNGPADENLRTFPVRIENDRLKISLKK"
     assembly_gap    56648..56747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(56770..57972)
                     /locus_tag="PKM27_02305"
     CDS             complement(56770..57972)
                     /locus_tag="PKM27_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNR06117.1"
                     /translation="MHRIVQWIGPCITPSLALLISCSSLEPAAPAEDELLDGPVEGLS
                     FEQNSRFLAGDRAFNDEVFTSPTGLGPLFVATSCGSCHAGDGKGHPFSSLVRFGQTDS
                     TGNRYLHAGGPQLQHRSIPGYAPESLPPGASHSTFIPPANTGLGYLELVPDEDLLAMA
                     DPEDANWDGISGVPNWAVLPAYIRPGSDKISRNGKYIHRFGKKASAFDLMHQTVNAYH
                     QDIGISSSFAPTDIYTGLDIEPEISDLNIHQVVFYLQALKAPIQRKPDDPKIKAGKSL
                     FTATGCEKCHKESLRTGPSPIAALSDKTFHPYTDLLLHDMGNALDDGYTEGNAKTYEW
                     RTPPLWGLGLSPDSQGGQYFLLHDGRARSIEEAILLHGGEAETSRNKFNQLNVEDREA
                     LIRFLESL"
     gene            58074..58733
                     /locus_tag="PKM27_02310"
     CDS             58074..58733
                     /locus_tag="PKM27_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNR06118.1"
                     /translation="MHSHTEENYLKALFNLANEKGEVSVNDLSKILAIKMPTVNSMVK
                     KLAGKKLVHYESYKPLRLTEKGKKEAGLIIRKHRLTEMFLVQKMGFGWEQVHEIAEQI
                     EHIHSPYFFDKMDEMLGYPKIDPHGSPIPDKTGKVDWIRYEKLSDCGAGETVRLGAVI
                     HSTEEFLKFLNSRKLHLGLKIKIKSVESFDGSMVVSYGKNSTETLSLTVSERLLVEKI
                     G"
     assembly_gap    58889..58988
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(59339..60175)
                     /locus_tag="PKM27_02315"
     CDS             complement(59339..60175)
                     /locus_tag="PKM27_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNR06119.1"
                     /translation="MKKKYQTFLLFCCLLGCFTLSSAQDSGGLRVAGLQMNVSDDIST
                     NKNRILTAIREAAAGKASFLITPEGSLSGYRSTFNQKELEPALNEVLGLARELNLGLM
                     LGTCFKDEEGLCYNQVRIYSPEGTFLGAHSKILVCSPVDKPGTGEMAQYTAGTLQTFN
                     WNQLRFGALVCNDLWATPGYTTILNPYLPLKLKEMGAQVIFHAINSGTSQRYRPFHES
                     SAELWALHLGIPIVEVNAAYGDKKGNAQSGLIDRQGARLVRVPDFGERLFFCDIDLEV
                     KQ"
     gene            complement(60248..61021)
                     /locus_tag="PKM27_02320"
     CDS             complement(60248..61021)
                     /locus_tag="PKM27_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNR06120.1"
                     /translation="MEILILCLASFSVSLLTFFSGFGLGTLLMPVFALFFPIDAAIAL
                     TGVVHLLNNIFKFILLNRKVDVTTVLRFGVPAILGAYLGAKLLLNLSAWSPLFEYQLG
                     GKLFYGTPIKLIIAALLVFFALMESLPSLKKIEFDKNKMVAGGLLSGFFGGLSGHQGA
                     LRSAFLIRSGLSKEAYIATGVLIACLVDVSRLFVYSEKYMHSELMENSILLALASFSA
                     FLGAVLGNLLLKKVTLGQVQVLVTVMLILLAAGLALGLV"
     gene            complement(61032..61904)
                     /locus_tag="PKM27_02325"
     CDS             complement(61032..61904)
                     /locus_tag="PKM27_02325"
                     /inference="COORDINATES: protein motif:HMM:NF005537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNR06121.1"
                     /translation="MEKIILALPGNEALAEKLSTHTEIKTGRIELRTFPDGETLVRLH
                     SSVENKKVFLICSLVQADTRSLPLYFLGRLCKDLGAWEVVLVAPYLGYMRQDMAFQTG
                     EAVTTSYFAEFISGFVDRLITVDPHLHRFKQLSDIYSVPSRVVRSAPAIASWIKIHIP
                     KPLVLGPDSESRQWVSEVASDAGASYEVLQKIRQGDRQVEIGLPDLNAYRGHTPVLVD
                     DIISTGTTLSTAAGLLEVLGFEKPVCIGVHAVFSEGAYASLKKSYAGQIATVNTIAHE
                     SNRIDIAPLIATHL"
     gene            complement(61913..63427)
                     /locus_tag="PKM27_02330"
     CDS             complement(61913..63427)
                     /locus_tag="PKM27_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HNR06122.1"
                     /translation="MEMHDHRSYNQLKVKSIGIDTYKEHAIYMRIDCEICNSEGFTSL
                     TRLQVSANGQTIVATLMAVLSNLVRHDEAGLSPEAMKKLGVREGDIINIHHLKPVRSF
                     AYVRGKMYDKFFLEDQLMQIIQDVAKGQYSNIELAAFVTACAGDNLSLPEITALTRAM
                     VSAGSSLSWSSPVVLDKHSVGGLPGNRTSPIVVSIVAAAGFTIPKTSSRSITSPAGTA
                     DTMEVITRVDLGIESIKNVVYNTGGCLAWGGAMELSPADDILIAVEKALDIESEGQLV
                     ASVLSKKAAAGSTHVVIDMPVGSTAKVRSHDDAKRLKQYFHEVGESIGLNVDTYISDG
                     SQPVGVGIGPALEARDVLSVLKNETEAPADLKHKSAALAGLMLEMVNYSEKGKGKADA
                     LQFIENGKAYEKFKTICLAQGAFREPPLAQYQFDVSSTKAGVITEINNRNLAKVAKLA
                     GAPKSPAAGIEFFAPIGKKVNAGDLLFRIHSESTGEMAYSKEYLASIEDLITIQ"
     gene            complement(63483..63950)
                     /locus_tag="PKM27_02335"
     CDS             complement(63483..63950)
                     /locus_tag="PKM27_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06123.1"
                     /translation="MKNILMISLLGILWITCKTKQNSASGPEQPAMENHQVHDHPDHG
                     LSLNQGAKWKSDVPTLENVKNLQSIVERAVNMQGPTQESYLKTAAELQAGLDKMIREC
                     RMKGPDHDALHLWLEPLIQQVSAFKESKTTEEASANLDLIQKQIHLFADYFEG"
     gene            64137..65534
                     /locus_tag="PKM27_02340"
     CDS             64137..65534
                     /locus_tag="PKM27_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNR06124.1"
                     /translation="MKTIIEPFKIKSIEPIYFNTREEREKILEEAYYNLFLIHSRDVL
                     IDLLTDSGTSAMSSGQWAGIMQGDESYAGSPSFYRFEEAVRTVTGLPLVIPTHQGRAA
                     EKILFHLLGGKGKFILSNNLFDTTRANIEFSGAEGIDLVCEEGKHPSFPAPFKGNVDT
                     AALQKFIEKTGAQNIPLVILTVTNNSGGGQPVSMRNIRETRAICDRHGIPLFIDACRF
                     AENAYFIKLREEGYAGKPVPEIVKELFSYADGCTMSAKKDAFANIGGFLALRDEKLAR
                     QCRNLLIITEGFPTYGGLAGRDLEAIAIGLREVMDEHYLQYRIRSIEYLANKLIDAGV
                     PIIRPAGGHAVYLDAKAFNTHIPPDQYPGQALACALYLAGGIRGVEIGSLMFGKYDAA
                     RRLVPAPMELVRLAIPRRVYTQSHIDYVAEVVIEVFRKKREIQGLEITEEAPMLRHFT
                     AKLRPVSGNGPAALF"
     gene            65631..66308
                     /locus_tag="PKM27_02345"
     CDS             65631..66308
                     /locus_tag="PKM27_02345"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNR06125.1"
                     /translation="MKTSLRYGVLFGALALMPLLNSCTKEESITEDYANFDVAAMRQR
                     VNQYPVASLSAEEINSLNFMREEEKLARDVYNGLARHWKVNIFTNIAASEQTHMDAIL
                     QLLNKYGLPDPIVSDGEGVFANEALRTLYLQLMERGSVSLLAAYQTGAYIEDLDINDL
                     NNASEFIDNEDILYVYGNLNKGSRNHLRSFYRNVLRLNGTYTPSFLSQETFDAIISGQ
                     METGPWW"
     gene            66341..66943
                     /locus_tag="PKM27_02350"
     CDS             66341..66943
                     /locus_tag="PKM27_02350"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNR06126.1"
                     /translation="MKTYWLIALVLMLAPSLRAQETARLSAEDIDAILFMREEEKLAR
                     DAYELFYVKWGVNPFGHIRFSEQNHMDQVKTLIVSYKLEDPIEKTADEPGKFENGQLQ
                     NLYAEVVKEGSTSMMAGLKTGALIEEIDILDLDARMARSQNADIRRVFSYLKRASENH
                     LNAFVRKLKVNGVDYNPVKMNKKQFDEILGGNERGCCRAK"
     gene            67179..68438
                     /gene="dgoD"
                     /locus_tag="PKM27_02355"
     CDS             67179..68438
                     /gene="dgoD"
                     /locus_tag="PKM27_02355"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HNR06127.1"
                     /translation="MKTKPDPKGISRRSALHSLLALTGSAALPVAARGHELTEGPSRL
                     LARDRTKIKITKLETFLVKPRWIFLKVHTDAGVVGLGEPLLEGRALTIQTAIKEIEPY
                     LIGKDPRDVVHHWQAIYRHAFYKGGPILTSALSGIDQALWDIKGKLLNLPVYELLGGP
                     TRDRVRIYGRASNAEQMKERVKEGYTVIKTGVAKQNPSRAVENPKFIKHAVENFASLR
                     EAGGDTMDIGIDFHGAIPPQTAKILIRALEPYQPMFIEEPCPPENVDVLADIAKGTFL
                     PIATGERIFTKWGFRQILEKGAASILQPDLCHAGGITECRLIAGMAEAYYAVIAPHNP
                     MGPISLASGIQLAASIPNFLVQEQVSLGEGYLKQPFKLEKDGTVLIPTAPGLGIELDE
                     NKLADKIGHDWKNPETYDATDGSVVDW"
     gene            complement(68795..69550)
                     /locus_tag="PKM27_02360"
     CDS             complement(68795..69550)
                     /locus_tag="PKM27_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06128.1"
                     /translation="MLDNLKILIHQGRQYIPDVTRAIVPGIFRGRPVISEVDVKEKEL
                     AELCPTNAISLNPLSIDLGKCTFCGECAFAFPDKISFTTDYMLATNDRNRLIVKSGQE
                     HPIKLDPVLVRKEIHGYFGSSLKLRQVSAGGDNSCELELNACGNVNFDMGRFGIEFVA
                     SPRHADGLVITGPITANMASPLQICYDAIPEPKIIILAGTDAISGGIFEGSPALDRSF
                     ISKYNIDLYVPGNPIHPLTFINGVLDLIKKQET"
     gene            complement(69566..71062)
                     /locus_tag="PKM27_02365"
     CDS             complement(69566..71062)
                     /locus_tag="PKM27_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06129.1"
                     /translation="MNFISVRNHQTIPLSGIPMLDYDSFLELNVFNLIREKGAHCVNY
                     FGFRLDGLIRLVCCIANDESHSIQVSSSAVPRMALPSFSKHNLVFEKFEREIHENFGI
                     AYTDHPWLKPVRFPFNRFDPGSRIADYPFFKVDSEELHEVGVGPIHAGIIEPGHFRFI
                     CNGEQIVHLEIQLGYQHRGVESLFLEKNKLLQRCTLAENIAGDSVVGHSLAFAHLWES
                     LCKHQTGDHLKMARTIALELERIAIHTGDLSAMCTDVAYQLGSAVFGRLRTPVINFFQ
                     KWCGNRLAKGLIRPGKSHFPFTRDLAIGLMDFLDEYENDFEDISKELFSLPSALARFE
                     KTGVLTTGQALAIGTVGLSARMSGVSRDIRASHPHDAYEALDHQPVLKTHGDVYARAR
                     LRKKEINQSINHIRHLIKILAAPEVYDQNPLKPAPDSFCVSLVEGWRGEICHCAITDG
                     KGELIHYKIKDPSFHNWLGLALAVRNNEISDFPIANKSFDLSYCGHDL"
     gene            complement(71065..72492)
                     /locus_tag="PKM27_02370"
     CDS             complement(71065..72492)
                     /locus_tag="PKM27_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNR06130.1"
                     /translation="MTGLYLAGGFTIGGLLFFTRNRRYMFLLILGFLLLQTGFTIYEY
                     AHLNTPELVFFTPDALAVLLLMVLSLISIPAFYHSRVFLISNRDHIRHRAIYFGAMVL
                     LITSISAAYLANHIAVAWIFVEITTLSASALIYHRRNNRSLEGTWKYVFVCAISITLV
                     FIGILFLSFSLPQAVANDLSFAALLKNADRLNLFWLRLAFIFIFTGFTAKLGLVPMFT
                     AGIDAKDKAPSPAGALFSSALVNVGFVGIFRFYGVVAQTPLHSWVNTIILITAFLSVF
                     VASVYILKVKNLKRLFAYSGVEHMGIVMLGIAAGGVGYYGAVLHIVCHALVKSSFFFQ
                     MGQVYRVYQSKNVYDVGDYFKYNLHGAIVLLLAFFSASAMPPSGLFISEFMIFRSLFE
                     ANHLLILAGVVLLLTVIIWAFAKNIFKLVFSPATGFNDKNLEIIKPVESLSQFVLLAL
                     AVYLGLNPPDVFVRLIEEAIHHLPR"
     gene            complement(72497..73126)
                     /locus_tag="PKM27_02375"
     CDS             complement(72497..73126)
                     /locus_tag="PKM27_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06131.1"
                     /translation="MTNVLLIIFTITLLYLGIAHRLLTYIKILAFQGILLFGIAFIEL
                     IEIDLANLSFVLLETVIFKTIAIPLFLQYVMKRNRITRDAEPYLPNFMSLIIITLIII
                     STFLLSNTIPDARISKIYFVVALSTLFTGLFIIASRNKLITHVMGYLVIENGVFILSL
                     AVGTEMPVLVNLGILLDIFASIFLLGIFVNRIGDVLRDVDVDQLSQLKD"
     gene            complement(73153..>74000)
                     /locus_tag="PKM27_02380"
     CDS             complement(73153..>74000)
                     /locus_tag="PKM27_02380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNR06132.1"
                     /translation="TKSITSGRKGPGIFQPLKDLARLFKKGSVYSQTTSFIFQLAPSV
                     YLASILMAVLVIPLGQYKGILSFEGDFVFFAYVLGAGKFFSILSAMDTGSSFEGMGAS
                     REALFSMFAEPAFFLLMGSFALLTGYTSFQQIFHSLHFGSSVSYVLMVLATYVLVMIA
                     LIENSRMPIDDPKTHLELTMVHEVMVLDNSGFDLGMILYGTPLKLAMYGGLIANLFMG
                     DFPLVLTIPIFLGLQIAFAVTVGIIESFMARFRMNHNAQYILTLSSVSLLILLGVLLI
                     LGQFN"
BASE COUNT        18862 a        17385 c        18390 g        18963 t
ORIGIN      
        1 catcagcggg ccaaagaggg cggctgcaag cccgaccgta gcgcccttgc ccatctggat
       61 ggccagagcg gaagccagtc cgccattttg cagtccgact tctatggcca ccgtcctgca
      121 atcctgcacc ggcagtttga acaacctggc cagattgtag cccaggaaaa aaccggacaa
      181 attatgcagc agcacagcga tcaacaacat gccgccgacc gtaacgaggg aatcgtggcc
      241 tgcggccgtg accaccacga ttacataagc gatgcccgcc atggacaaga ggggcaatac
      301 tttctgcagc ctctgggcta ccaccttgag ataggtatta atgagaaaac caagggctac
      361 gggaaggatc acgatttttg tgatgtccca catcattttc cagaaatcga tttcaatata
      421 tccgcccccg agtgttttca taaaaaaggg ggtcaggaag ggagccagca gggtggcgca
      481 ggaggtgatg gtgatggaaa gcggtacatt ggccttggcg atataagcca tcacgttgga
      541 agccagcccg ctcggactgc ttccgataag gatgatgccg gccgcgatct cggggggaaa
      601 tttaaaaatc agggtcaggg ccagtccgac aaaaggcatg atcgaaaact ggcagaccag
      661 cccgataaaa acccctttgg gatttttaat aatccccata aaatcctcgg tagtcatggt
      721 ggtccccatg ccaaacatga tcacctggag catgggcagt accagggtgg tcagcttgac
      781 gccgttcact tcaatgaaca actgggggaa aaagtaagcg acaatgactg caaatacaat
      841 gaaaagggta tacgcgtatt ttttcatgct atccgggaga tttagcggca aaatacaaaa
      901 accgatacca aagtgaaggg gtgacttgag gtcatccctt cacttgcttg ttttttttcc
      961 ggttgccgaa attttttaac aaggtcactt ccctggggct gagatgacgg aaatggcccc
     1021 ggggcaggtc ttttttattc aaaccggcat aagccacccg gtccagtttg atgattttat
     1081 aacccaggtg ttcaaaaatg cgacggacaa tccggttttt accggaatgc agggtgatac
     1141 ccgcttcatt ggggcctgcc ccgtcaatat aactgattcc atccacctcc gccctgccat
     1201 cttccaggag gatgcctttt ttgattctaa gaagttcctc gggatcgagg ggcttatcca
     1261 aaaccacatg gtaaatttta cgcacttcgc tgctgggatg gctgagcatc cgggccaggt
     1321 cgccgtcatt ggtcagcaga agcaagccgg tggtcatccg gtccagacgg ccgaccggat
     1381 aaaccctttc tttcactttt tttcgcacga ggtccaggac ggtcaggcgg cctttttcat
     1441 cggaggcggt cgtgatggta tccttgggct tgttcataag caggtatacc aaattggatt
     1501 ccggtttgag caatttgccc tgatacgtta cccggtcttt gggaccaatg cgcagcccgg
     1561 gttccgatac ggcttttcca ttgacccaga ccttgcccgc cctgatgagt tcgtccgcag
     1621 cccggcggct agcaatgccg caggaggcca catatttgtt gaggcggacc tcttccgggg
     1681 tatcaggaat ggatgatggg cgtttgggct gatcggattt cgacaaggtg gcctgggatt
     1741 gttagatcat caattgtcaa aggacgctaa tccggttcaa aaccaagaat gatgctgaat
     1801 ttgccataac tgccgaaccg ttttttatcg ttttccggaa gatctttgtc aaaaccaaag
     1861 ccataatcaa aaccaagcaa accaaacatg ggcaggaata cgcgcatgcc gagacccgcc
     1921 gaacgcttga ggtcaaaggg attgtaattt ttactcccga tccaggcatt tccgccctcc
     1981 aggaaggtga gcaaaaagat ggatgaattg ggattgaggg tcagcggata ccggagttcc
     2041 atggtaaact tgttgaatat ggtggcgccc ccgctggtag cggcattggc agccaggtcg
     2101 gccacttcat aacctctcaa ggcaagaatg tcccgaccgg tgatcccata gttttggttt
     2161 gaaaggccat cccccccaag ttcaaaccgc tcaaagggca ccaggccgat ttttttattg
     2221 taaaagccca gcaagcccat cttggcctgc attttcagga ccagtttacc cgccagggtc
     2281 gaataccact gggcgtccag cctccatttg tggtattcca cgtatttaaa aacctcctgg
     2341 ggcgtcccgt ccgtgttcag gtttttacgg aaaagggaat agggcggagt aagggacacg
     2401 gtcagcgaga tattggatcc agaccttggg aaaagaggat cgtagatggt gctgcgggca
     2461 aaggtttgtt tgatgctgaa gttgttgtaa ctccccccga tgatgggctg gccggtgcgc
     2521 ggatcgataa aggaccggtc gctgccaagg cttccgtcca gcctgatgtt ttctatgttg
     2581 agcgtggtgt tggtgacaaa attgtcatcc ggccatttca actgggaacc gagcccgata
     2641 taacccctgg cgatctggag tttcccgaga ccaaacaatt cctggttaaa tttggtaagg
     2701 aaggcgccta aggtaaagga gtttgctttc ttccctccca gccagggttc ggtgaaagaa
     2761 aaattgtagg attgatagaa tttgccgttg gactggcccc ggagggagag tttttggccg
     2821 tcgcccgtag gcagggggtc ccattgtttt ttattaaaca aattgcgcat ggcaaaattg
     2881 ttgaaggtca ccccgagggt tccgatcact ccctggaaac cgccccagcc ggcggagagc
     2941 tccaattgat cggaaggttt ttccaccacg gaataggtaa tgtccaccgt gcccctttga
     3001 gggtttacgt cggtatcaat gccaactttc tcctggtcaa aataaccgag gttgatgatt
     3061 tgccgctggc tccggatgat gtcggagcgg ctgaattttt caccgggccg ggtgcggagt
     3121 tcgcgccgga tgacgtgttc atgggtccgg tcattgccca atatattgac tttgtcaatg
     3181 gtggcctggg ggccctcgta aatgcgcatt tcaaaatcga tgctgtcgtt ttcgatggat
     3241 acctcgatcg gttccacgcg gaaaaaaaga tatccgtcat ccatgtagag gctgctcacg
     3301 tcacggccat cctggctgta tttcaaccgc tggtccatca gctcctggtt gtatacatcg
     3361 cccttattaa taccgagata agcattgagc tgtttttcgt cgtggatgga attgcctttc
     3421 caggtgatgt ccccgatata aaagcggggc ccctcctcca ccaccagttt caggttgagg
     3481 gtgccgtctt cgttcctcca gatgctgtcc cggacaaacc gggcgtcgcg gaaacccacg
     3541 gtattgtaat actggatgac tttgaccttg tccttttcga ggtcttcctt aaccagcttt
     3601 gatttgccga aaattttccc cttttgtttg gtttcggaaa gtttgccgcg cagttttttg
     3661 tcggatacga gctgattgcc ttcaaaaata atctggtcga tttttacccg gtcgttgggc
     3721 gaaacgtcaa attccaggac caggccgttg cgcattttgg tatctctttt ttcaagcact
     3781 ttcacatcca catcataaaa tcccttgtct ttaaaaaact gcttgatctc attgacggcg
     3841 gtttgttttt tgttttccgt gaccacgtcg ttgcggttga tgaaccgctt cagtttttca
     3901 ttaaggtcct cgtggtataa ttttttgatg ccggtatagc tgtagcgggc caggcggggc
     3961 ctttccagga ggcggatttc caggtcgacg atattgtccg taaccctggc ctggatgatt
     4021 tccacctggg taaacagttt gagcttccag agcgcgcgta tggctttctg gatttccggc
     4081 ccgggaatat ttattttcga accgctttta aggccggtga ccccgatcag ggcgttggca
     4141 tcgctgttga aggtaccggt gacgctgatc tggccgactt cataagcttt cggcaggtca
     4201 taatcaatga aaggcagtac cgtatcctgc tgggcccgca ggccggtagt caggaccagg
     4261 ccaaggatta aaagcaaacc tttccacatc cacttcatgt tgttcttcct gagtttatta
     4321 ttaacgaatg ccggcaaacg ctcatcactc gtttttgttt cgtttaacaa tgttttaaat
     4381 gttgggggta tggtccatca tcaactgatc ggaagtcatg ccataacgcc tttccctttg
     4441 ctggtattcc aggatagcct gccagaagtc ctttttctgg atatccggcc aaaacacagg
     4501 ggtaaaataa aattcagtat aagcgcattg ccacaacaaa aagttgctta gccgttgttc
     4561 tccactgctc cggatcaaaa gatccgggtc gggatagtta tgggtctcga gttctgactg
     4621 gacggtatgt tcgtcaattt ggtcgggatg gagcaggcct tgttttacac ggctggcgat
     4681 ctgccgggtg gcgcgggtaa tctcccacct tgaactgtaa ttcagggcca ggatcagggt
     4741 catgccggta ttctgggccg tatcccgcat tccttttttc aaggcctgga gggaagcggc
     4801 aggcagggcg gaaatatcgc cgatggcttc caggcggata ttgttttcat tcagggtttt
     4861 gacttcctgg ccaatggttt cgaccaggag ggccatgagg gcattgactt cctgttccgg
     4921 gcggttccag ttttctgtgg aaaacgcata gagggtcagg taacggatac ctatttcagc
     4981 agcggcttcg gtgcaggccc ttacggcctt gaccccgttg tgatgcccgt aaatgcgggg
     5041 catgccctgc cccttggccc atcgtccgtt gccatccatg atgatggcga catgctgggg
     5101 aagcctggaa acgtcgatcc cggattttag atccatggca agtttacaaa attgggtgca
     5161 aaagtactta aaatccaaca agttagccga taaatttttg agcaatcagc tgtcagctat
     5221 cgagaacatc gggacggcct tcagcaccat tgctgaaaac tgatggctga tggctgaaat
     5281 ctctctactt ttatcccaat gaagtatttg atcaaatcgg gcattggcct gatcctggcc
     5341 tggtgcctct atccggcctg caatccggta tcctgtccga aaacgaagaa agaactgatc
     5401 agccacttca accaatttgt cagcagcacg gtcagggaca aggaatttta ttccatccgc
     5461 gaatgggagg ccaaagacaa ggaattctcc ggataccttc aggattgtta tcccttgctg
     5521 gatacgacct tttacctgga tgacaaacag cggttttgga gcgatgcgct ccggtattat
     5581 tttaaaagat atgacaaccg ggtaaccacg gaattattga acaataaaaa tcccaattcg
     5641 gtcattcttc aaacccagat caggtccaac tgggccaatc cggatgaagc cttcacccgg
     5701 atttttcagg aaatgaccgg attaaaattt gacgaagccc tgaaggcagc cagggattcc
     5761 attgcggaag aagatcaata gaaaagaacc catgtatgac gtcatcctga gttttattac
     5821 cgctttttcg cttacttacc tggtcatccc cagcatcatc cgggtagcca aatcaaaaca
     5881 tatttatgat gaaccggatg agttccgccg gtcccacgac gctcccgtcc cacgactcgg
     5941 cggtattgcc atttttgccg gagtggtttt gtcggtgacc ctatggacgc ctttccaggt
     6001 atttgccggc cttcaatata ttctttgcgc cctggtcatt atttttctgg taggttttca
     6061 cgatgacatt cagccggtat ccgccgtcta taaaatgata ggtcaggtcc tggcggcctt
     6121 tatcctggta tttaaggcag atatcaaact gaccagcctg tatgggattt tcggtattta
     6181 ccaattgccg gagctggctt ctatcgcgct gagcattttt accatcatcg tgatcatcaa
     6241 cgcctttaac ctgatcgatg gcatcaatgg actgtcgggg agcatctccg cgctgatttc
     6301 cctcaccctg ggcatttggt atttgctgat caaccgtctg gaaattgcgg tactggcctt
     6361 ttcccttgcg ggtgcagtca ttgctttcct gaaatacaat tttactccgg ccaggatttt
     6421 tatgggggac accggctccc tgctcatcgg cctggtttgt tcgacgctga ccattaagtt
     6481 cattgaattg caccgcgaac tgaccgattc cgcctattcc ttcaaagcgg tgccggcagt
     6541 cgccattggg atattgatcc ttcctttatt cgacacgctg cgcgttttca ccatgcggat
     6601 cctgaggggc cgctccccga tgagccccga ccgcaatcat atccatcacc tgctcatcga
     6661 ttccggcctc aatcacatgc aggccacggg catcctggtg gccgtcaacc tcctgtttat
     6721 ttttatggtc tggtccctgc aggaaatcgg caccctgaat ttattgctgg tgatccttgc
     6781 cttcgccacc ttattgagcg ggatgttgtt tttgattgca aggaaaagaa ggatgggaag
     6841 gtaaggcatg gatcaaataa atacggggat acctgctcat ggacgaaaat caaccagcca
     6901 caacagggag tttctgcatt gaaatgggtg aagggccaaa aactcaaaac gcatcatcga
     6961 gcggcagtgc gtaacccaaa aggtccatga aaagaaagta atataaggct gggtgacagg
     7021 tttaaataca gcaagtgatc ttaactatta ggtatattcg ttcaataaat tgaattttat
     7081 gcaacgaaag gactttatta aaaactccgc cttattggca gtagccataa gcacatcggg
     7141 ctttattcgt tttgacggca aaagatatgt cggggattgc gaaacgactt ctgatgtatt
     7201 gggaccattt taccgccccg atagccccct tcgaaacaac tttgtaattc agggtgagaa
     7261 aggcgagccg attgaacttt cgggtaaaat caaacacgaa gattgcacaa cgccatacca
     7321 aaatgcaaaa gtagaacttt ggcattgcga ccacaacggc ctgtatgaca acgaatcccc
     7381 cgatttcaaa tatcgtggca cggtctattc cgatgaggaa gggaattatt cgttcaaaac
     7441 tattttgccg gttccctatg gtgagggcaa caactatcgg cctgcacatt ttcacctgat
     7501 gatttctgct gaagggtatc aacctttggt gacccagctc tatttcgtgg gcgacccctg
     7561 gatcgggaag gacgagatgg cttcctctcc aactgccaaa agaaggattt tagaagtaaa
     7621 aaatcttaaa gacgggagta aaaaagtggc tttcgacatc agtatgtcaa aaaaacttgc
     7681 tgccgaccct gcaatattcc atcggttgga gggcatttat attgaagaaa aagaagcagg
     7741 caaagcaaag gagttgttca aaaaagataa ccagttgtgg ttcaaatcca atgggatgcc
     7801 ttttggccag aaccttgaat atgtttccaa taataccttt atcataccag gcattccacc
     7861 caagtataat ttttcttttg tttttgagtt catgccttct ggcgcagtga aaatgatgga
     7921 ggtttttcaa gatgagaaag gggaaaaaca agtaatcgtt tcgattaaga aacaatagta
     7981 taatcatttt ttctctcttt cagtatccgt tgcgaaagat tagtttctcc cgtgagcagg
     8041 agcttcaccg gaatgtacga ccttcagacg tttaagggga gagggaattt tcaaggggtt
     8101 cggatgccct ctaacaccac cattgtttta gaacagacat cttaccaacc taccacttcg
     8161 tcaataaaac atagttttgc agccatgatt cgaagcgttt gttttgcggt cctcacgata
     8221 ttgttttttg tttttcctgc ggccgcccaa tatgggcttc gctgcgacgg tacgcgttac
     8281 cttcaaccgg tttttgactc catcagtgta agtacggtcc agtttggcaa aaacaaagga
     8341 ccggcaggag acagcatcac gctccggatg gatgtatacc agccggcagg agatcctgca
     8401 gcggcccggc cggctatcgt cctggctttc ggaggggttt ttatcaccgg cgaccggacc
     8461 cagctcaggc ccctggccct ggaatttgcc aggagagggt atgtggtttc ctccatcgat
     8521 taccggatct ggcctttttt tgtactgggt tttccggatt cggccgacat cgtggacatc
     8581 agcgtgaaag ccatgggcga tatgaaggcc gccgttcggt acatgaaggc cgcggcggac
     8641 caataccgga tcgataccag cctggtattc agcggcgggg tatcctccgg ctccatcacc
     8701 gccctgattt ccgcctacct cgaccctgcg gacagcgttc agccttatct gaaggaagtg
     8761 attgaacaaa acggcggcct ggaaggcagc agcaatccgg caaccctctc cttcagcagc
     8821 cgggtacgcg gagtgctgaa tctctccgga gccatgctgg atctttcctg gctggatgaa
     8881 ggggaaccga tcctggccag catccacggg acggccgacg actttgttcc ttatgcttat
     8941 ggccttgccg ccggcctggt tcatctttac ggtagcagcc tgatccaccc ggctttggaa
     9001 gataaaaaaa ttaaaaacca actgatttca gtgcccggag gaggacattc caatatttat
     9061 gatgaacccc ggtttctgcc ctataccgac agttttatcc aggtagccgc ccgcctgttc
     9121 catccgatcg tctgcggcga ggtcagcacc cggctcaggg aacaacaggt ccaaacggct
     9181 ggtttttatc ccaacccggc gaccgaccat atccagttga tcctgaaaga caaccgacct
     9241 ccccaatacc tccgtatcac ggatgccatg gggcgtagtg ttgaatttat agggggaggc
     9301 agccgtattg attttcttcc cggccggctt tcccccggct tatacgtcct ggaactcatt
     9361 tatgaaggaa accgcaggga aaggggaaaa ctgatgataa agtgatccgc ccttgtcctg
     9421 ctcagaaaag gaccacccgc gacacgaaat aaaaatgaac agcctggaaa ttgaaataga
     9481 aagagtcagg attgacgacc ttgacctatt gcagaccatt ggcaggcaaa cgttttacga
     9541 tactttttca ccggcgaaca ccgaggagaa tatgaataaa tacatggatg aagggttttc
     9601 gtccgaaaaa ctggctgctg aaataaacaa caaggactct gaattttatt ttgcaaaact
     9661 agagggtaaa gtcatcgggt acttaaagat caattccgga tcttcgcaga ccgatttgaa
     9721 agaccacaaa gcccttgaaa ttgagcggat ttacgtgtta aaggaattcc aggggaaaaa
     9781 ggtgggacca ttgctttttg acaaggccct ccagattgca aaacggaaga aagtagaata
     9841 tgtttggttg ggggtttggg aagaaaatca cagagcgata aatttttata agaaaaaggg
     9901 cttcatggaa tttgataaac atattttcaa attgggcgag gacgagcaga ccgacctcat
     9961 gatgaagctg gaattgaaaa acagataggg catctggccg aatttttaac gggacgaaat
    10021 tttcccgttg cggatcggcc acccgtatgg gatttcaaaa taaaggtcta tggcgtcagt
    10081 tccttcttat acgtcctcct tcttttatgc tgacggtact cataaccttt ccagagcaac
    10141 tgcacctgca cgttggattc cagttcggga ttggactcca cttttttgat tccgaatttg
    10201 actgcgcttc gggtgatttc ataaaaaatg gtggcgtgca cccctttgtt gcgccaggcc
    10261 ggatccgtgc cgatcagata gagatcgagc cggtcgtttt gttttaaggc ttgcaagagg
    10321 tggtaccagc ccgtagggaa taaccggccc ccgctttttt gcagcgcccg gctgatcgaa
    10381 ggcaggctga tgccgaaagc gatcagccgg ttgttttgat cggccaccag ggaaagaaat
    10441 tcaggcctca ggatttttga atacttccgc acatagagat tgacctgctc gggttcgaaa
    10501 gccacaaaac catggagcgc gttatgggtt ttattgatca gctcaaatac ttccagggcg
    10561 tattttttga tgtccgcccg ggagcggatt tccaccgaat ggattttata tttttccttg
    10621 agtaattcgt tgaagcggat gaaccgttca gaaacttccg tcgggcaatc cagttcgtgc
    10681 tcgatccagt ctgccgcctt gacataagcc agtttctcca ggtgctttgc ataataagga
    10741 taattatacc aggtggcgga ggtaggcagt tcttcaaaac cttccacgag caggccggct
    10801 ttgtccaggt tggtgaaact catcgggcct tcgagttcgg tcattcccct tgccttcgac
    10861 cagtcttcaa tttcgcggag gagggtccgg ctgacttcct cgtcgtcttc aaaatcaaac
    10921 cagccaaacc gggctttggc cttatggtac aattgatttt ccctttggtt gatgatgccc
    10981 gcaatgcgtc ccaccgcccg gccgtcccgg taagcaagaa gcaggcgaag tttgcagtat
    11041 ttgagaaccg gattgtgcac cggcgaaaaa atatccaatt catcgcccca aagcggaggg
    11101 acccatacgg ggtgtttcct gtacaattca aacggatagc cgatgaattt ttttagatct
    11161 gagcggtttg agggattgat ttcgaccagg cgcacggaca tattcagata ttccttttca
    11221 gcaattccgt cagcaacgaa tttaaaattt ctttcctgga agaactggcc tccacggcgg
    11281 ccagggcgtc ggaagccttc tggttgtatt cctggctcag ggctttgagc ttatcgccgg
    11341 ccccatatcg agtaaaaagt tcaagcactt taggaacctt gtcctcctcc cggccttcgt
    11401 aaagatcgag cagggctttc cggtcttcgt cattggcttt ttccagggtt ttgataaaaa
    11461 gcagggtctt tttgttctgt aaaatatctc ctccgttcat ttttcccgtc tggtccccga
    11521 aagcatcgag gtaatcgtcc atcaactgga aggcaagacc ggcatttaaa cccatatggt
    11581 acaggtgttt ctgatcttcc tcagaagcgc cggccgtcat ggcgcccatc cgggcggcgg
    11641 cggccagtaa aaccgccgtt ttgtcgcgga tcatatccag gtattcttcc ttttttatag
    11701 tggtccgcgt ttcgaaattg atatcccttt gctggccttc gcaaatttta atggcggttt
    11761 cgttcatcag gcggatgatg gattgagcca gtttggattt gtattgattc agaatctcat
    11821 aggtgtggat cagcatccag tccccggaaa ggatcgcggc attgatatta tacttggagt
    11881 gaacggtcgg cttgcccctg cgcagcggag actggtccat aatgtcgtcg tgcatcaggg
    11941 aaaaattgtg aaaaacctct atggccagtg cggccggtaa ggcctcgttc aactggtttc
    12001 tgaaaagttg atggacggca agggtaaata ccggcctcag ccttttgccg ccaagctcca
    12061 tgagataacg cgcgggttcg tacagttcgg gaacgggtcc cctgaagggg tggtccttcc
    12121 ggtaagcttc aaaaagggtt aacaactgtt ctacataaag catggcggaa aaataaggag
    12181 aaatggggag tcggcaatca ggagtcagca atcagcagtc agcagtcagt gggttggatg
    12241 tcctacaatt cagcaattga accgtaatca tgcaatagac tagcttttaa aggctggaaa
    12301 actgcatgta agcactgcat tcgatttggt ctaccgaggc cggtaaccgg agcttggcaa
    12361 gcttgaatta gtaggctgtt ttttcattgg cacggttaag ttcttcggat tgtaaaaggt
    12421 gggctatcag gtcatgtcct tcaagttgat ggtccatagg tagctccaac tgggagcgat
    12481 aagccagata catgccttgc aggcgaagtt gctgcatttg ttgtaaagtt tgatttgtat
    12541 tcattgtgat ttgaatttca tgtgagaaga tttacggata atgctccttg ccgcggatgt
    12601 tgtcatggtc aaagaaaacg gaaggcgaga tttgggtggc atgtcggtca aggcctcttt
    12661 ccagtatatt tttgatcagc gtatagttga cccgagaacc ttgcaaggat cttttgcagg
    12721 gtgcttccag gcggtctgaa ccatattttt tttgaagcac aatcatgccg aagcaggctt
    12781 tataattttg ttcgatatag atacagttat ccatcatgag tcttgcggcc tgataagcgg
    12841 aggatccgat cctcaaagcc tgttccagta tatcatcccg gttccaccct ttaatttgtt
    12901 ccatgcgttg atgatgcgga ggcatatgat ctctcagggt cgtgtatgct ttattccgga
    12961 cctttcgcaa atgcaaggcg attctctcct ggtccagata gatctccacg gtggttgcat
    13021 cgtaaagtac ttatactttt ttccctacat accgataagg aacactatag tagtgatggt
    13081 cctcggtgag ttgcacatgg taattgcgtt gcacggtcaa tcgaacggct tttttaggta
    13141 agaatggcag agccggcaga ggtttaagca ggagtttttc ctgctgatca aaaaaatacc
    13201 ggcggctata gggggtgttt ttatagggct tttcgttgag cagctccagt tgttcgaaca
    13261 gggctttgtt caggtccttc aagctggtaa aatttcggtg gcgtaatggc ccataaatat
    13321 gattgtacac aatatattta cagcacgttc caccatggct ttatccctcg ggctataagg
    13381 acgtgtagcg ctgaaggtgg ttccgtaatg ttcactgagt tggaggcaga tatcggcaaa
    13441 ttccggctca tagcggtctg accgttttac cgcggttctc atgttgtcac atataattgt
    13501 ggatgtgact ccaccataaa acttagtcat cgagttggtc aagtgggtaa agtctgcagt
    13561 ttgttgggtg tgaaccgcct gacaataaat tagaccactg aaaggaagaa ttgatataaa
    13621 tacttcacac ttaataagtt ctcctgtttc cagatcagtg tagtatagtt tttttccggc
    13681 aaaatcaatc atggtcatgt cacctggtga atattccagg tgcatggaca gatcacggtt
    13741 cttgaggtat tgcttcagat ggaaacagta ccggctataa ctgtatccgt ccggatgagt
    13801 gtccaagtat tcccgccacg aaagctgtct tgtgacaccg gttttagtca gttcatggcc
    13861 tgtcgtgctg aaatgaacag tgacttgtcg catcagttcg gcttccatct ccaataagtc
    13921 attggagtag accttcgagg ctagttctac atcatctggc ccctgacaat cagaaagcaa
    13981 cgaaaggtac tgtcgaacgc tattacggct gatacctact cgacgcgcta tctcccggat
    14041 ggggataccg tcacgatgaa gttgaaaaac ttgttttaat ttttccattg gtatcggttt
    14101 ttgtgccatg cttctggttt ttagaagcaa atgactacaa aagccctttc ctaattgccg
    14161 aataaggggg gtcaatcccg aagcctcggg agcaggaatc tcccaggggc accctagatc
    14221 agacccaaaa ggggtgggtc aacatgcaag aatctccctt ggagacccca gatctgactc
    14281 ccagcaggtg ggtcaaaatg caggaatcct cccttttaga cctggatctg atcccttata
    14341 ggcgggtcaa catgcaagaa tctcccggtg atacccctgc tcagtcctcc aaagggtagg
    14401 tcaacatgca ggaatggtgg gtcagcttaa acaagaatgg tgggtcaaca tggtcaggaa
    14461 tctacaactc tgtgtatttc ttttttgaat tgaagatcca ctgtccccga agacagacca
    14521 aattggttcc catcatcaat ggctgggaaa tggtcaccgg ttgatccgga tttgatcttc
    14581 taaacaattt actaacacgt attatttgtt ccttgatacc caccttcatg ttcgcccgga
    14641 tcctgatatc gaaggggatc tcctgttcta tcagatattt tatccagaga tctccagcat
    14701 cttgattcgg gcaagatgga atccaatgaa aaataagcgg aaaatgttaa tgaatttact
    14761 atcttgaact ttcgtcgcca tatctgtgag atttaagttt aaaaacctga atttcaattc
    14821 acaatatctg gcgattttaa tttatgtcat gtgctaaggt gaatgttatt tttatatttg
    14881 aaataataaa cgcatagtat gttgagttct tcatagaatg tgagccaatc gagaagagag
    14941 aaaggaatac aaaaaattgc ctattcgata ctgtactggt taaaaactta ctgctccttt
    15001 gaaagctagc taacaccata agccccattc acttctccca tggcttattt gaatgcgttt
    15061 agtatactga aggcaataag gagtgaatta ttgtattttt gtgaaaccta tgtaactaat
    15121 tagttacgaa cgtgaaattc aagcaatatt actaatgatg caaaacttca ttctcatttt
    15181 aggtttactt ctcggatgta tatttaaatt aaatgcacaa tcagctccgc ttaaagatag
    15241 agtctttaaa gatgacagta tttcattaaa tctcgataag agaccacgca cagctttatt
    15301 tcttggacta ttattacctg gagcaggcca agcctataat ggtaggtggt ggaaaattcc
    15361 attagcatat ggagcatacg gtttctcctt ttatatgatt agttcgacaa gaaagactta
    15421 cagagtttgg aataataact ttaatcaagc agtaagaaca ggcaaaaagg ttgaagtggc
    15481 tcctggaatc gaatttgatt cacggcaaat aaaacttatt cgtgaccaag ctcgtgataa
    15541 caaagatagg gctgtttttt taatgattgg catccatgga ttaagtgcat tggaagcatt
    15601 tgtggatgcc catctaaaaa atttcaatat tgatgatgat cttggagttc atttctattc
    15661 accttcggtt cctggattat cactctgttt tacatacaat cttcactaaa atgtttaaaa
    15721 atccttatat cagctaaggt caacctcaaa ttaccctaaa gcttctaagc agaattttat
    15781 aacaatttaa atacttaaca attatgaatt atttagggcg aaaatgtaat tattcttttg
    15841 attacaaatt tttattgacc tgttttattg tttttcttac attatttgct tgtcaaaaag
    15901 aaagtgacag cataattaag ttagaaatca agcctgaggc aagtcaaagt ttagaaacac
    15961 attataaact agcacagata tttgaaaata aaaagattat agaatttgag ggatataaag
    16021 aatttcaaag agtagctaaa ttttgtatag ataacatcga cttattaaca ggcaaaatag
    16081 aaaatggtga aattattttt gaatctcctt ttagagattt cattatggct gagatagaaa
    16141 tgaataaaat taaaagcgaa gaagattggt ttgtttttaa aaataaatgg agtgaaaaag
    16201 tgacaataag ggatcattca atagatccaa agtatttaat gtattccaag aatattttgt
    16261 gcaatttaaa tggcatatta aaaattggaa ataaatacca taaatatttt gatgacttct
    16321 atgcttcatc caatgaatta aatttacttg tgaattataa agaaacggat agatataaag
    16381 tatcacaaaa tgttactgta tatgaaatca aaaattcttg taatatacca agatcaggaa
    16441 gtagggcaaa cgatggtagt atatcctgcg tgtatacagt taatccaagt ggctgcaaca
    16501 aaagaagaat acaaggattt ttagactggg aatcagaagt ttttgatgaa ccgggattgc
    16561 caacagccta tacattcaat tctcaatcaa ctacaagata tcaatattta agtatagtat
    16621 ggttaaatcg agacgctgat acaattaatg ctacagtttc tcatagaata gatatagtaa
    16681 gcccaccttt tagtgatatc ttttctggaa gtgatgtgag aacaaattca aaactagcca
    16741 ttgcatatgc caatcccctt ccttttgccc acccatttac cgtttctcaa cataccagca
    16801 cacatattgt gaaaagagat gaatgtaata caataaatct ttcttgcaac aattattctg
    16861 ctaactaaat cttttttaaa actttaagta gactttgtta aatgaataat ttttaatgaa
    16921 gtctacttaa atcaaaataa ttttaatgtg ttaaaaaaag gctgttattc ttaaaaatga
    16981 attcaaaaag aataatatca ctatagcttt acctaattcc atgtattggt aattcacagt
    17041 cctcgtggaa tataacatca ggaataggtc atttttcatt tattcacaaa cctcaatttg
    17101 tgttaaaccc aaaacctaca ataggatagc ttgaaattgg tggcaatccc cggtattgct
    17161 atcttaatgg gacgaactat gatacacaca aaagatgcac gagttagcga acgaattcac
    17221 ggaaagaaag attagtccct ggggtgaaat taaattcaac agttcagcag tttatcagta
    17281 gctgagtttc agccgaacaa aagccacatg gctcatggaa gaaaaccggt tttggtcttc
    17341 cacaaaaaag ctttgccata ccaggtcggc atccaagttg ttggccaggc tgtatcgcag
    17401 ggatggaagc aggatcagca ggttggtgcc cggagcaaaa aggatcccgg catttcccga
    17461 aaacaggggg gtgaattccc gggatacggt tccgatgacg ttataacggg ttggcatcag
    17521 gttttcggcg gagaggttga ggtcgatttc tgcccagttt tgaatgggtt tgtattttcc
    17581 ctgtttgttg aataagatcc ccaggttaaa aaaccaatta ttgtccgtga ggtaattcca
    17641 ttccatggag aggttgaggt tggagggact gttgttttta ttgaaaaaaa actgggcttc
    17701 gcctttaaaa cccaggttcc tgatattacc cgcccaaccc atgcccaggg aaggccggtc
    17761 gaaataccag ccgccgatta actgaaaatc gtatttccaa cgattgaaag tatattttat
    17821 cgcggtgacc ggtttgtcct taaaatggtt gtaggctatg gcccactcca catttgagtt
    17881 tcccccggag aaatactgaa tgcgggcgcc atcgttgccg ggcctttctt cataatccac
    17941 atccaggaaa ttgtaggtat taaaaatgtc gtttgggttc caggtggtgg tcatgcccca
    18001 gttgatgcgc tgcctgccca gcctgaaatt ccagcgggtc ccggtgtatt caacattcag
    18061 ccgttccaca ctggtatgga gcaccatgga tttgccgctg atccaggctt tctgcagatc
    18121 aagtccttcg tttttatttc gcagcagggc cacataaccc ggtatcagcc tgacttcgtc
    18181 gccccagatg aggcggtttc ggaatgaaac cgaggcacta aacctttcat taggcatcca
    18241 ttgcatattg agccggtgat ggaggatcga ggtgatatat tcttcctgat tgatttcgtc
    18301 gagggtcagg taggccagtt ccttgagata gcctcctgcg gtaaattttc cggatttttc
    18361 ctcaaggctg tcctgggcgg ccagtccggc cggtaacatc agaaataata taaacagtac
    18421 ttttttcact caatgctcca gtggtttaaa ggcggtatca gaatctatcc ggccgtcaac
    18481 cagggtgacc acccgacggg cccggtcgat cacgcgtttg tcgtgggttg aaaaaataaa
    18541 agtcatattt tcttcctggt tgagtttgta catgatatcg aggaggttgg cggcggcttt
    18601 agaatcgaga ttggcggtag gttcatccgc caggataaac tggggcttgg aagccagcgc
    18661 gcgggccacg gccacgcgct gttgctggcc gccggaaagc tgtgagggcc gtacattcat
    18721 tttgtcctcc aggccgatct ctttgagcaa ttgcaggacc cttttttcac gggttgtttt
    18781 gtcaacgccc tgaagcagca ggataaactc tatgttttct ttggccgtga ggacagggat
    18841 gaggttgaag gactgaaata caaatccaat atgatgaagc ctgaaatcaa tgagctggtt
    18901 atcggaaagc tgggtgatgt ccaccccgtt gatgaaaatc tttccccccg tgggccggtc
    18961 cagtcccccg atcaggttga gcagggtggt ttttcctgaa cccgagggtc ccaccagggc
    19021 ggtgaattca ccctgttcaa tatgcaaatg gacatgattg accgccggta ccgggatggc
    19081 ttcggggttg taggtcttac tgacatcgtg cgcatcaatt acggtctgat tcatactttt
    19141 ttcatcaagg gtttatcttc ttaaggcttc ggaaggccgc atattcacgg ctttgagaga
    19201 ggggagtatg gaggaaagca aggcggtgat cgcgacgatg gacaaaacca tcaccagttg
    19261 atcataagga aattcgggga aaatccaact ttcaaaacca aaactggaca tggcttcttt
    19321 tgcaacggca gcgatattta tcccgtggcg ggcaaaatac cagatgagga tccaggccaa
    19381 tcccatgcca acgggcactc cggcgagggt caacaatatg gtttctgata aaatgagccc
    19441 gaacagccgg gccttggtca atcccagggc ggtcatcata ccgacttccc gggtacgctc
    19501 gagaacggcc atcagcatgg tattgatgat gccgaaggcc agggccagca tgatgatgat
    19561 gatatagata aacatgtatt catcgagcga aaccaccatc aggttggttt cgggggatat
    19621 ttctttccag ttttcggtga gcagggcggg gaagcggctt tttagccttt gatgaatggt
    19681 atcgagctgg tcatcggatt gcaggatcag ggtgatttca tgggattgcc cgggaatggc
    19741 caggaggtca ttgagttcat cctgcctgag gtagacggta cgttcatcca gcggggtatt
    19801 gatggtttga aacaaaccgc atatgcggaa ggcgccggcc tggatatcgt tgtccttgtt
    19861 tgtaagggtc agtacgattt tgttgcccag tttgagcttt aatttatcgc tcagtttccg
    19921 gctgatgagg atctggttct tttttgcagg atcaaacagg ctgccttcca cgattttacg
    19981 ggatatatgg gatacctgtt cttcttcctc cggccggatg cccgttacct ggacgccgga
    20041 ggtcccggtg gcggccgaaa gcatggctgt ggataccgtc ctcgcggcaa gagcccggat
    20101 ttcagtccag ccaagcagtt gctgaatcag gctgtcggaa cccggaatga tataggctgc
    20161 gccgaagtct tttttaaatt ccgggtggtg aatttgaagg tgcccgactt cctggtcaat
    20221 gaccgtcctg acccgggact tgagtattcc tttataaatg ccaagaatcg caactccgga
    20281 aaaaacgccg agggctaccg aagccatgat gatcaggctt cgcattttat tgcgccagat
    20341 atttctccat gccatggaaa aaatcagata ggccggcctg cttttgtggt tcatcttatc
    20401 ttctcatggc tttgacgggg tccatccgca ggattttata caatggatag agtgaaagaa
    20461 ggagtccaag gcataaaacg atcaggccct gggaccagaa aatgcccggt tcggtcgatc
    20521 ccggcatgac cgcttcaaaa ccaaatcgtt cataggcctt tccaaattca cctccaaagc
    20581 ggatgggatt atttttaaaa taaaaagcca ccgggatgct tgccgccagt ccggccaggc
    20641 aaccggtgat caccgtgaat accgattcaa tcaccatcag caggatcata tttccttttt
    20701 tcatgccgag cgcgatcagc atacccattt caaactgcct ttccaccatc atcatggtca
    20761 gggtaccgaa gatcccgaaa gcgatcagca ggtaaaggag gtattggatg atttgcatgg
    20821 tgaaatggtc ggtcttcatg tgctggacga tttcgggcat catgtccttc caggtcattg
    20881 ccaccagccc ggaatcaagg cgctttttca atccgagtac agtttcttcc aaaaggtttt
    20941 ggtcatgcag ggaaattaca acggaggtgg cccggttttc ggcgccataa agttcctggg
    21001 ccgcgggaag ggacaaaaaa gccagtcggt tattgaggtc cggtgcgccg tatttgagga
    21061 tcccttttat cgggtacttc cccgcagccg tagcgccatg aaaaccctgg ccgatgatgt
    21121 atacggtatc ctccaggctg agttgcagtt gttcggcaag gccttcggtg agaaggatgg
    21181 cctggtctcc ggattcaagg taccgccctt tttttaaacg ttcctggagt tgggtgactt
    21241 ccgactctct catggggtcg atccctacca ccaggcatcc cttggtaata tcccccgacg
    21301 acatcagggc aaaggactcc agtcttggac tcaaggcatc gatattgttt tctccggaaa
    21361 tttcggcttt cagggaatcc gaccattcca ggctgttgtc cagcacctgc tcatcccaat
    21421 acccagcctg ctggatctgg atataccccg agtaatagcc taccaggttt tggatcaggg
    21481 cgccgaaggt ccctttccag aggctgttgg ccaccacgga aaagatcact gcaaaaaaaa
    21541 tggccgacat ggagatcagg gtgcggttgc ggttgcgcca gatgtttttc caagccagtt
    21601 tgatcagcca catcaacgga tgtttttcat attgttaatt gaaaaaaagc catcggcgat
    21661 cggttggtca taaaccactt ttttgtagcg catctcggtt ttctggttcc ttttttcgac
    21721 ggggatgatt tcaaatcgcg tgggaatcag acgcccgccc aggagggcgg gatcataggc
    21781 attcatgata ttgaccagtt gtccttcttc gtcgtaaaac cgggtatgca attccataaa
    21841 gtccttttta tcaatacata ccatcagttt tccccagact acagcggctt cgggtttagg
    21901 gaccatccag atggtatgac agggtcgacc caggatcagg gtatccgtcc ccaggctgtg
    21961 ttcaaaatca cggatcacgg aagattcccg gaccaggtcg tcgttggtaa agtcggtccc
    22021 catccagctc tggctcatca tggacggggg cagtttaatg gttttttcca gggtgggtat
    22081 ccaattccat acttccttgc ctcttttcag aaaagtaatg ccgcggtccc gggccgggga
    22141 ttggatcagg atcattgcaa aatcattgcc cttactccat acccggaggt ccatttcgcg
    22201 ggaccatgag gggcggatgg ttttgatgtt catttcaacc tgggaagtat tgcctctcat
    22261 tttagcatcc gcctttttta cgatttccag tgcagtctgg cccgttgata ggatgggtag
    22321 gattaatagg attggtagga ttgataggat tcgtannnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22441 nnnnnnnnnn nnnnncattc tgatgattca acaatatttc gggtcaggga attcatacat
    22501 tcatggattt atattcggcc ataatacgct ccgcaagctc ctgctttaaa ggttcaggga
    22561 taaacgcagc ccggatggca tggagattgc aatccaggaa gtggcggatt ccccaggaaa
    22621 agtggctttg cagtttttca tattcccggg taagggtgat atccgacagg gtccggttgt
    22681 ccgtattgat ccccagggag actcccagcc ggtataattt atcgaccgga tgctccccgt
    22741 aggtttcgac gagattcatc tgaacattgc tggaaggaca gatctcgagg tgaatgtgtt
    22801 gatccctgag gtattgaatc aaggccggat cttctatgct cctgacccca tggccgatcc
    22861 gggaaggccg gagttcgcgc aggcattccc atacgctgtc cgcccccctt gcctcgccgg
    22921 catggcaggt gcaggggatg ccggtttgat gggcatagtc ataagcttta cggtgttggt
    22981 ccaccgggta accggcttcg tccgcagcca ggtcaaagcc agtcacatga ctgtttttaa
    23041 aggtctgtac cagttccacc gtctccatgc tctgggcttc cgtaaaatgc cgcagggtac
    23101 ataatataag ccgggcgtgg atgccgtaat gcttgatcgc ggaagcggtg gcttgttcga
    23161 caatgctcac ggcttctccc ggagtcaaac ctttttcggt atgcagcagg ggggcgaaac
    23221 ggatttccgc gtaaaagacc ttgtcctttt ttaactgttc aaaaaggtca aggactacca
    23281 cttcaagttg ttccgccgtc tgcatgtatt taagggactg aaatacgggg gtgatcaatt
    23341 ccatcaggcc tgaacatttg gccggagcca taaactggcg gtggtattga tcccgggtga
    23401 tttgcgggtc caggaggtgc agggcatgat aactcaggga gcaatccagg tggagatgca
    23461 attcaacttt tggcaagagg gagatcgccg gggaagaatg atctgattcc attttattaa
    23521 tatctccttt cagccgcaac gggataaacg ggttactccc ggacggccct taaactgtct
    23581 tttttctctt ttttatccaa tcttctgaaa tatccgcgga ataaaaaatt ggttttcagc
    23641 gcttccatat tctggttgaa agaagaggtc cctttttcaa tattctccag ggaattttga
    23701 agagagtggg tcatggagga atcccgcagg atggcctgga caggcccttt accctggtca
    23761 atatggtctt tcagcgattg cacgacctgg ctgacctgcc gggtgatgcg gttactctct
    23821 tctccggcca gctttatttt ttcagaggta ttcagcaggt tggcatagat caaggtgtcc
    23881 tggaacagtg cgcccaggct tcctttgccc cggctcacgc tgtcaagcag ggtattgacc
    23941 tcagcggcgg aaagttgcaa ttgatgggct gcccggcgaa tgtcagcgag ggcggctctg
    24001 atgtcgtttg ggatttcgtc agaggtgacc agtttgacca gggccgggtt ttggttgata
    24061 ttggtgagca gggttttcaa ttcccgcgcg atgtccgcga tgtcctggtt ggttccggac
    24121 aaggtgcgca gggcggcgtc ggtatccagc gcttctttgg aggccaggac gttgtcctct
    24181 tccaccaggg ccgcctcttc tttcaccggt tcgatgttga cgattttatt tccgacaaaa
    24241 ccgtcgttcc cgatagagac ccgggcattt tggcgaatga tgtttttaaa tttattgtct
    24301 atatccagga ctacttcgat cagggtatcg ttgaggatgt tgatgctttt caccgctccg
    24361 gcctggatgc cggcataccg cacattgttg cccggctgga ggccctggat atgctgaaaa
    24421 tgggttttca gcaggaagga aggatgaaaa atattccggt tttttccgat ataatagagc
    24481 aggatgacca ggaaggccat accggcaatg acgaaaatac ccagcttgac ataattaaaa
    24541 atcgtttttg ccatctcgat gcattagctc attattgttc aaaaaacggt ttcacgttcg
    24601 ggtccgtact cgacttcagg cggtcatagg tgtccagggc ataacatttc ccttcaatca
    24661 gaacggctac caggtcgctg gccagccgca cacaattcat gtcgtgggaa atgatgaggg
    24721 aggcggtgtt gtatttattt tggatttcca cgatcaggtc gatgatttcc tttcccgtga
    24781 tcgggtccaa tcccgtagtc ggctcgtcgt agaggataat atcgggtttg aggatcaggg
    24841 tgcgcgccag ggctatcctt ttgcgcatgc ccccggacag ttccaccggc atcatatcca
    24901 cggtatgggc cagtcctaca tcttccaggg cttccatcac catgtcgttt acctgagcct
    24961 gggtcacctg gatccagtgg cgtcgcaggg ggaattcgag gttgtccctt acggtcatgg
    25021 agtcatagag ggcattgctt tgaaacaaaa atccgatcct ggccctcagt tcatccaggg
    25081 cgaaatggtt cagttcggga acattttttc caaatacgaa aatctctccc gcatccggcc
    25141 tgagcaagcc gatgatgcat ttgatcaaaa ccgatttgcc ggaaccggat ttaccgagca
    25201 cgacgatgtt ttggccttta tagagagtga gattaaaatc ctggaggacc cggttggacc
    25261 cgaacgattt gtaaagatgg gaaatgacga tcacctcctc cttggactgc ggagcagacc
    25321 gggaaatccc cggcgccaca ccggaaatga cggtatcgtt tgaatccggg tcagccatat
    25381 cctattaaat ccgtgacctg aaccacgagg aggtcagtga taaaaataag cacggaagaa
    25441 acaactacgg aggaattggc gctccggccc accccttccg tacccttgct gctgtggtaa
    25501 cccttataac aacctatgat tcccacgata aagccaaaaa agaaggtttt aataaaagcg
    25561 gggaaaatat cggcatagat caggttggta aaaaccttgt tccaaaacag agaccaactg
    25621 gtgatccctt taatgttcac ccccagatag gacccataga gcgcgatgca atcgctcagg
    25681 aggaccagca ccggcaacat aaaggtggtg gccagcaccc gcgataccac caggtattta
    25741 aaagggtttg tgccgctgac ctccatggca tcgatctgtt cggtgacctt catggagccc
    25801 agttctgctc cgatgctgct tccgattttg ccggcaaata tgagggcggt gatgatgggg
    25861 ccgatttccc ttataatgga aatgcccacg atggccggga tctgggactc cacgccataa
    25921 ttgaccatcg aaggccgcaa ttgcatagtc atcaccaggc ctatgataaa cccggtcaat
    25981 ccgatcaggg gcagggattt gtaaccgatg atataacatt gcctgaaaaa ctcggaaatt
    26041 tcataacggg gcctgaacca ttcattaagg aaccggcccg taaatacggc gatccttccg
    26101 gtttcgtcca gaaatttttt cagtgtggtg tcggtggcca taggctatcg tgttcaaaaa
    26161 taggtttttg tgccgagaat agagattgaa ggagatcaga cgggagggtg acggaaatca
    26221 gggtgcggat gacgcggtta atgggttaag gttttacgca caaactcgcc attgattact
    26281 ttttccatca ggtcctgagg cacataataa tataggttgg acgcttcaaa tccaatacgg
    26341 gaatcccgcc ggataatctc aaacaattga acggccattt taatctcttc atccaacaga
    26401 tggtttattt gttttttcaa catttgttta ttggccgaag agaggcttgc tttgcccagg
    26461 gaatcccgca acaggataaa ccgggcctgg ttggcagagg atgcgaagtg cagccaggcc
    26521 gctttggcaa taccccagtc tttgagcgcc gtttccttat tttctctgcg gaccgttatt
    26581 aatacttttt taaactgatc gagccccttc ttccatccgg cggcaacctt ttcgaattgt
    26641 tttgcgaacc catctgccgg ataggggccc ctccacttat tgagatcatc ataagggaaa
    26701 ccgaccatgg aagcaaggta atgggtaggc ttgggataca gaagattgga aggtccatat
    26761 tgctgggggc ctgaatagag cacactttgg tgatatggaa attccaggaa ggcattgctg
    26821 aaggaagtcc atgctttccg ggcaaaggga gcggcctggc tcccataacg ggaaatggcc
    26881 aggctgtcca gcacggcttt tggatccgca ccggggtcct gtgaaaatcg ctgtgcaatt
    26941 tccaggttgg gtgaaggata accgcctaaa gtccaactga gcataaagcc gtcaacatcc
    27001 accctggaca aatttgaggc atgttgtgcc accagatcca gggcggggat ccagggaaca
    27061 gacgagagct cccaactgtt gttgaattgg acttttgcca ccgttttaag acctgcttct
    27121 ttggccagcg cccaatgcct ttgcgccctt ggccccgggc caacagcaga catggaatat
    27181 tcattgacct gcgaaacgat ccctcccctt tcaatgggtt tggcccattc gctcaccgac
    27241 atcagccaga ccgatttcgg caaacgggca ataatatccg gtgcatcccc atggccatgc
    27301 catccccagt cccaggcgat gactttagcg tccggattgg cccggtggac cccaagttcc
    27361 atggcttggt ttacttctgc aataatatct gcatagtcgc gtttgctgca tcgcggacag
    27421 ccagcctgat ggtcatgcga agcacaatga gttaaatttt ctgaggccgt aatggtaaaa
    27481 atgccaccca gatccggcac ttctttaaat acataggcca gagaatgggt gatccagttc
    27541 aggactgttt cattgcttgt gcacatcgtc atataatttc cctgttgtac tcctgccagg
    27601 gacgaccggt ttttgaaaaa agccaaaggc atggcacgcg gttcgtttac gtagagggat
    27661 attttgatgc cgtatttttt ggcttgttgt gaaatccgtt tgaggttcgc caggcgggtt
    27721 tgatgtcctg caccgaattc aggaaattcc ttaccgccag gagccaactg gttcaggacg
    27781 atgtgcatcc agacgccgtt tatcccttta gacgccagct tggcaagcag tccttccgga
    27841 tagggatcgc tgtggtcatc gatgagagga tctccaaaaa caccaaaata agaatagata
    27901 aaacgcaaac cctctgctgc cgaaaggtca gcgaccggga tcgggacaga caaatcccgg
    27961 ttacgcatat cctgtataaa accgaaaagg ggttcggccg gaatcttaaa cgatttttta
    28021 aatttttttc cgacaagttg tttaatttcc caggctatgg cccgttcctg tggattgggt
    28081 gcagaatatt tcagggggag gcaggccggt ttgagatttc caaacttatg aaataaaaag
    28141 tcatcctctt tgagcgaaaa ttccaattcc tgaggagtca tatcagccag gttaagcaat
    28201 tgatcataag ggaggagatg ccagtttcgc ctcaggacgg tgatgtaaag ttgttttcta
    28261 aagcctggtg gtataggttg ggccggcgga agacccatag aggcggcaat ggagtgaatg
    28321 tcttcagccg tacagcctat cgtttgtgcg atccggtccg ggtcaaccag attccagttg
    28381 cgccatatca cggcatacat gcggtttgga aaatgttcaa acgggagtgc tggcggatga
    28441 gatccaaggg gcaaggatcc tgcgaaagga gttgatccat gacaaggaaa ttcagtgcac
    28501 aaagaagaac acttaaagct ttcatgatta ttttacaaat actatattaa gaggctgtca
    28561 tgggttaaat atacagaaca ggtatacggg tttccctata aaaccgcatt aatacaaatc
    28621 catcatgggg agaatgccaa tgggttaaat atataatttt gatggaccgc tcacccggat
    28681 gacatacaag ggcgggtaca accatgtcag acgaagagaa aaaaatccct ccggacgaat
    28741 ttaatcggtc gctttcccgg ttattaaatc aaatcgccga atgttaccgc tatcttggcc
    28801 ccgcccagca tttccgcgcc aacgcttcgg gaaatcttat aaagggaagg gcctggctcc
    28861 gggagtaatg gtcaagaaca gcccggcaga attagctgcc ggaggggaca agacccttca
    28921 gaaagcgatt gaacaattga actggagcct gagcttagtc tacaattcag attttcacca
    28981 aatggttact gcaggatgat tttttgggaa attatatgat ccgagccaat catttccaca
    29041 ttatacagac ccttaccctg gtttttaaga tcaagttcaa cctgggcctc tccaggtcta
    29101 atttgttttt ctaaaacgat gcgcccagtg atgtcggtca cgcgcaacaa ggccccgtgt
    29161 tcaattctgc cgcccacttt gaccaggccg gtgctgggat ttgggaatac ttgcatttcc
    29221 gaattaaaca cccggctggc gggatggtat ttgtagattg gttttgtatt attaaaattg
    29281 actcggacaa gagtctctgt tattcgagag gcctcagcaa tgggcaggtt ttcgccttca
    29341 gacctcctga atccctccag ccaccaaggg gacagttggt cattaatata tgaataggcc
    29401 gcagaatagc cgcccctggt cccgcctaca atatacttat ccttggattt tatcaaaaag
    29461 gcttcctgaa cagggatggt attgtcgagg ctgccactct ccgccaccgc accattataa
    29521 aatacttgac gcgcataatg catcgtccca ccgctgctga ccggcccggt gcgccaggaa
    29581 gtcgtcacat aggggccttc caaaattgca tttgagatcc aggaaaccgg agtcccggcc
    29641 aaagcagtat tctgaggggc atgtaccatg ctccacttta cggtgccacc atgattaatc
    29701 atggcggtat aagcgccgga atgactattg gaaaaacagg aaccggtcaa aataattccc
    29761 accgggaggg aaattcctcc ggccggaagg atatattcga acaattcttc aaattcagta
    29821 tacgtatatg caccgctcgg tgaggggggc aggacaacgg ccgtggacca ttccagattg
    29881 cccgaatgat cgattttggt gatcctgctg ccggtacttc ctataaagta tccaccgtcc
    29941 caagaagcca cgatggacca tccaaaaccg gaaatgggaa taatttttgt ccattctatg
    30001 cggccctgag ggtcgaattt cacaacagcc aaatcgtaaa agttgctgga accaacagcc
    30061 acccagccgc catttttagc gttggtcact ccgccaaaag taatgccgtt gggcagatcg
    30121 gacacccggt cccagatttt acggccctcg ctgtcggtca ggagggcata ggccttttgg
    30181 ccggattggc tgcctgaaga aactgcaaag attttactct tgtcatgact caatacaaaa
    30241 cttttccaaa atacatcaag tcttgaaaat cctgaggcgg tatgcgattt cagaaacaag
    30301 gatttaccct ggtgatcaaa tccctggacg tataaactcg tattgttggg ttcaaaaaat
    30361 ttcgagccca ccatgacgta tccgtattcg ttgggttgca ccttccagca ggaatgatcg
    30421 aaggaacccc agttgtatgt ggtttcaatt atctgggcct ggaccgcagg agcatgactg
    30481 acaaaaagga acatcaacag gcatctgaat aaatatttca tagttgaaca tttttaagct
    30541 caattcacaa ataatttggt ttgttatgtg cattcccata ggtcgcacat aaagactgat
    30601 tgtttaaaat ttgtaaaaaa ggaaagtgtc cgttatgcac aaatctttta accatcaatt
    30661 caagaacaca taaacgaaat taataaattg ttttgcaaag ttcatccgat ccctaatata
    30721 aacttaatca cattgagtct ttactgtatc ctaaatacat tggttcgtct gcaacctttt
    30781 caaaacctat gatccctaag agattgaaaa tcagttatcc agatagcgaa cccggttaac
    30841 ctccatcctc cttgtccctg aatggattaa attcaaacca atgactaact ttataaagta
    30901 tggtcaggga aaagaataat agtgccccag gtgagttcaa ccgatccttg tccattttat
    30961 taaatcaaat cgccgaatgc taccgctatc tcggccccgc ccagcatttc cgcgccaacg
    31021 catacagcgc cgccgccaaa accatcgggc agatgaaaac atccgtcctc gccctggatc
    31081 acgatgtcaa aaaactcagc cagcttaaaa tcatcgggga aagcatagcc gaaaatatcc
    31141 tggaatatgc ggataccgga aagatctcga ggtacgaaga actgaaaaag atcgtgccgg
    31201 tcgacttgct ggaactcatg caggtgaggg gaataggccc ctcgaccata aggctcctgc
    31261 atgaaaagct gcatataaac aacaaggaag aacttttaaa ggccctggag gaaaataaac
    31321 tgtcggatct tgaaaacttc ggcccgcgaa aaattgaaaa tttgaagaag gcattaaaaa
    31381 tattcaaggg agaccggcgg atgccgctgg ctgaagccgg ccgggtcggg aaagcccttc
    31441 tggaatatgt gcttaagatc ccgcaggtcg aaagggccga actcgcaggt agcctgagga
    31501 ggagaaagga gacggtaggg gatatcgacc tggtcatcca ggcggaggac cgtcaccgca
    31561 atgccatcac caaccgcatc gtggcatacc ccctggtggc ggaggtgatc gcccggggcc
    31621 ctacccgggt gagcatggtc ctcaacccgg ggcacgtcca ggtggatatc cgggtggtaa
    31681 acaaagatgc atttggttca agcttgctgt attttaccgg ttccagggag catacccttc
    31741 agttgcgccg ccaggctcag aaaatgggcc tgaaactgaa tgaatatggt ttgtttgaag
    31801 tgggaagcgg gaaaaagctg gccggggaaa ccgaggaagg ggtgtacaag gcgctgggat
    31861 tggaatattt ggaaccggaa gaacgcgtgt agcggacggt tttaaccgtc cgtcgtcctg
    31921 gacactcgtg taactgacgg gttaaccgtg cgtcgtccgg gacgaataaa ttcgtcccct
    31981 acttgttatg cgcgataaac aagggcaatt ttaattcact catcagcaca tccgccatgc
    32041 tctccctgaa ccatctggaa acctttcccc tgcgataagc gcccaaaacc accagggtcc
    32101 ccgccggttt ttctttgagg tgattgacga tttgctcatc ggggtatcct tttaataccg
    32161 tgtatttggt ttttggaagt tggcgtttca ggtattcttt tataaaccgg ctgtcgggcg
    32221 tgtgcatggt ttgtccgccg gtgctgatgg aaaggatctc cacattggac ccgtcatttt
    32281 tgccaaaaat gtagttgaac attttaaggg caaaaaccga tacggtctgg ccatcgtaaa
    32341 ggaccaccac ctgttcgatg gctttaaatt ttgaaggcac gacgatcacc ggacattgta
    32401 cgtcttccag caagtgacgg ataaaggaag tcggcgcttt ttcatttcgt ggtttaaagg
    32461 tttccgtgcc gttgatcacc aggagatcgg catagatgct ttcatgaagg aggtccttca
    32521 acgcgttgtt tttgtcgtgg tgaatggtaa actgcagctt ccctttttta aacacctcgt
    32581 ccaacttttc ggcgctggct tttcggtggg cgcggtcctg gttgtccagc tcttttttta
    32641 atttctccga cacgccgtcc tccgtgatca ggtcgtagat cttataaccg tggtattccc
    32701 ggtcgtccag gaagaccgcc acgatatggg cattgagttt atgggccagg tccacggaat
    32761 aatcctgggt ggataccgaa tatttcagcc catcaaaagc agacaggatc tttttcataa
    32821 ataaaaattg tgactataaa gttaaatcct tagaaaggga atcttctgac cccaacaaga
    32881 tgaccacctg atttccgtca ggccaatgcc ctttccctca ttcttcgtca atatacatct
    32941 gcgggtattt ttttttaaaa gctttcgggg ctacacatct ccaggcgatc agcagggcat
    33001 ctttcagtat ttccggattt aatttttcca gttctgccgt ggtccagccc aggcggcccc
    33061 aggcattggg cacggggtaa atggcgcccc ggccgacaga agtgaagaga tcctggtatt
    33121 ccttatcaaa cttgagcgtg catctttttt gaggggcatt gacggtaacg aatatttttt
    33181 tattgacgcg gtaggagacc aggtcaaaat gaggatgctg ttctgtttcg gggaaggaga
    33241 gtgcaatgga ttttatgaaa gtggctgaca gcatgatgga gcggtaaaga gatgaaatta
    33301 acaaacagat ctgagctggc ggaaaggtac gctcaaacgg aggcctccgc actgcggacc
    33361 catcttttcc ttaagtttga tcccaaattc agtccaaatg aaaaagacca ccatctccac
    33421 gcttttttct ttaatgatgt ttgccgcttt tactcagaat cgacctgcgc cgggcccgcc
    33481 accacccgcg ccatccgctc ccgcgccaaa agaggaaaaa aaacaatcca ccgacatcgt
    33541 cttccatccg gagtcctcgg tagtcaccca gcatgagatc accatcaagg gccagcgggt
    33601 gccctttaag gccactgccg gcacccttcc ggtctgggat gaggagggca aaacccttgc
    33661 cggattgttt tacacctatt atgagcgcac cgatgtccgt gaccgggaca gccggcccat
    33721 cattgtttcg ttcaatggcg gacccggcag cgcttccgta tggatgcaca tcgcctacac
    33781 cggtccggtg gtgctgaata tagacgatga agggtatccg gtgcagcctt atggatttaa
    33841 agacaatccg cattccatcc tggatgtggc agacatcgtc tatgtggacc cggtcaatac
    33901 cggatactcc cggccggtca gcaaggatat tcccagaaac cggtttttcg gggtaaacgc
    33961 cgacatcaaa tatctggcgg aatggatcgg gacctttatg acgcgttatc agcgatgggc
    34021 ttctcccaaa tatctgattg gagaaagtta tggaaccacc cgggtctccg gcctggccct
    34081 ggaacttcag gaaagccagt ggatgtattt caacggggtt attttggtgt cgcccaccaa
    34141 cctgggattg gtgcgcagcg ctccgcaaac cgccgccctg cgcctgcctt attttgccgc
    34201 cacggcctgg taccataaaa aactgccggc tgatctgcag caaaaggatc ttacggccat
    34261 gcttccggaa gtggaacact tcacgatgaa cgaattgctg acggccatta ataaaggaag
    34321 catgcttccg gatgtggaaa gaaaaaacat cgcccagaaa atggctcgtt tttccggcat
    34381 cgacgcggaa gtctacctgc aaaacaacct ggacgtatcc acttccctgt tctggaaaga
    34441 attgttgagg gacgaagggt acaccgtggg caggctggat tcccggtata aggggatgga
    34501 cctcaaagac gccggtgagc ggcccgatta caattcagaa ctcaccagct ggctccacgc
    34561 cttcaccccg ccgatcaata tgtacctcag ggaaaagctc aattataaga ccgacctgaa
    34621 atacaatatg tttggccccg tgcgtccctg ggacgacagt aacaacaata ccgggatgaa
    34681 cctcggcaag gccatgtctt ccaatcctta cctccacgtg atggtccagt ccggttatta
    34741 cgacggggcc tgcgattatt tcagcgccca gtacaatatg tggcagatcg atccggcggg
    34801 caaattcaag gaccgggtca gctggaaggg ataccggagc ggacatatga tgtacctgcg
    34861 caaggaagac ctggcgagca gcaatgagga catccgccag tttctgaaaa tggcgggtac
    34921 aaaaccgggg cagccggcga agtattgaag ggtcaaaggg taaaggttaa ggggaaaggg
    34981 agaaaaagga ttgaaaaagg agacaggagg aagggaggaa gggagaaatg gaggaaggaa
    35041 gggaatgagt caaaacacta actttggaat cctgctcctg taaaaccatg aacctgtcag
    35101 ggactggccg gaatgtcaag cggacgattt attatctgac cgcggttctt tgtattgcag
    35161 gcattgcctt tgaatgttca aatcagccgg cagaaatcag ggaccccctt gaaagaaatc
    35221 ttgttttagc tgccggcaac cggcctgagc tggaaaaagt aatcgaccat ttccgaaaag
    35281 atccggacag cctgaaactc aaagcgaccc ttttgctgct gagcaatatg gaggataaat
    35341 ttcattattc cggggaatgg ttaaggcagg cagactccct tttcttcagc cgtgaagggg
    35401 ggcttggaga agaagatctg cagttactcc gggacagcgt ttatggactc ataggaagcc
    35461 cggcccacgc ggacatccgt aaaatccagg atttgcagca cctgacctcc tcctatttaa
    35521 ttcaaaacat agaggatgcc tttgaatcct ggcaaaaggc tccctggaaa gaggcggtca
    35581 gttttgacgc tttttgcaat tatatccttc cttacaaatc gtataatgaa tatcctgaaa
    35641 actggaggcc cttgcttcgg gcccgttata ataatttgct cctggaaaac ggggttcccc
    35701 aagccatgga agacatttgt tgcgcactgg tcgatgagga aaaaaaatgg ctcaaatatt
    35761 ctgaggaacc gcggggttat ccgggagcct taagcatcag ctccattctc aaactgcaaa
    35821 aaggatcctg cgtggaatgg tcagcatacg gagcggaagc ggcacgggcc tttggcatac
    35881 cagtggccat tgattatgtg cctcaatatg gtaaccggta ttctacgcac aaatggaacg
    35941 ccctggtcct gtcggacacc tcctttcttt cattcctcgg cgcggaaagc cggccagggg
    36001 accatgcccc gatccgggag ggggaaacca agtttgccaa agttttccgg tggcggttgg
    36061 gggctgatcc ggaaagtttt gcggtcaagg cccgaaaggc cgggatcaaa gccatccccc
    36121 cgtatcttga agacccgagg ataaaagatg tgaccgccca ttacacgctg gtgtctgatc
    36181 tgagctttga agtgaaaggc gacccgggta tgcccctgta cctgtgtgtg tttaaaaact
    36241 gggactggga ggccattgcc gggggactga ttgatcagga caaagtgcgg tttccgcaga
    36301 tgggaagggc cgtgctgtac atgcctatgt tttaccaaaa cgggcgttac cggcccgcag
    36361 gcccgccggt cggtctgatg gcagataata aaatacaggt gtaccggcca ggcggcgaag
    36421 gaaatctggt cctgaaacgc aaatatccct ttaaaagaac ggaggaatgg cttcgggaac
    36481 attccctgca gaatgcacgg tttgagggaa gccatgatcc ggaattcacc aagcctgctt
    36541 tattatatta tgtacttcga aatgtaaggc cttatcgggg ggcccgggtg aataacctgg
    36601 ctctcaagga ccaatggtta tatgattcac tctggagaga ggcgccggtg cgtcataagg
    36661 atagtttcca atacgtccgg ttgttattca aaaaaggaaa tgaattcagg cttggggaga
    36721 tcgaatttta tgcagatggg aagacccgac ctctgtcggg caaaatcctt ggtaatattc
    36781 ctcaagcccc cctggccttt gatggtttcc ccgggaaatg catcaaactg ccaaaagaca
    36841 cctctcaagc ccgctgggtg gggttggatc tcgggaaaaa aacggcagtt gaaaagatcc
    36901 gttttatacc ggcggatgat gacaattttg tggaggccgg ccaaacgtat gaattgtttt
    36961 actggaaaga caagtgggta tcggcgggaa agcagaaagc cctgagcgat tcgcttgaat
    37021 atcaaaaagt accgcaggga accgtttatt tgttgcgttg ctgggactgc aacaatctgg
    37081 aggaaagacc ctttacttat gaggggggta agcagatatg gtggtaatgg ggaactatta
    37141 aaaattaaag aattaagaat taagggaatt aagatggctg tatacacgat aaccgccctg
    37201 aacgaatccg actatccccg tatcctggaa atctgggaat cctccgtccg ggccacccat
    37261 cattttttaa aggaagagga tattcaatta ttcaggaaat ggattccgct gcattatctg
    37321 aaagctgtgg aattatcggg catccgggat gaagagggaa agctctgggg tttttcggga
    37381 atccacggtc aaagtctgga aatgttgttt atccatgcgg accagcgggg aaaaggattg
    37441 gggagacaac tgattacgca agcgatccgc acccgtcaga tcaacaaggt ggatgtgaat
    37501 gaagacaatc cggaggcgat cggtttttat aataagtttg gcttcagggt tgttgggcgt
    37561 tctgaactgg acggcagcga gaagccctat ccgatactgc atatggaacg catgaaatga
    37621 ggggcaatgg gaataggatt caaaaccatg caatagattt tctattacaa gccagaatgc
    37681 ctgcaaatat tggcacttcc tcccatttag tcagatggga acaaaaaaag gtgatttgaa
    37741 aggagccccc ttcagggcgc gtattcatga ccatcctcag caggaaggat gacctccatc
    37801 atcgcaggca gtttgtgtat tgtggatcgg atggtcttat ttaaaagggg aatagctatt
    37861 gaattgacgc cttgcccgaa ccatccccta taaaagtttt gccgctggaa aatccccgga
    37921 ggccttttca gaaaagggtt tccggccatg gcctggtatc gtaagcatcc ggctcgctgt
    37981 tttcggaatg ccggccgatg cctgccttgg aaaacagaac aagtcctccg caagggtaca
    38041 tttttagggc tcccggatga ataaaactta tctgatcagc ttcatcttga ggtttgactg
    38101 ccatcattca aagtccggtg agtctgatcg catctttgtt ttatcaaagg acccggtttt
    38161 aaaccatcac taaataaaaa ataaaggggg tgagggatta aaaatcaggc tctttgcaaa
    38221 cttgattatt taatttttta aaaccttttt atgaagctgc tacattttat tactatccta
    38281 ttggcattat ttatcatctc ctgccaacaa gatccacaag tcagaaccac agaagaagaa
    38341 ctcaccggaa tcactgctga agtcaaacaa aaaatcatca acctcggatt taatcctgaa
    38401 aactccttca aaatggaagg aggttacatg gtcgaggggg atattttttt gaccgaagag
    38461 gagctccatt ccctgcccaa caaccgtatc cctgtactgg agcaataccg taccaccaac
    38521 ctggtgaccg ttaatggaac caagacgatt tcgatcagcg taaacaagga cgtggccagt
    38581 tttacgcagg ccctggattc cgccatcgca cgttacaatg ctgaaaacct gacccttaaa
    38641 ttcaagcggg tcagcagcag cggaaacatc aagatttcca aagccgcctc ttcggccact
    38701 tacctggcaa attccgggtt cccgtccagc ggcaggccgt acagcaatat ctctgtgaac
    38761 ctgacccttc tgggtgccca gccgtttgga acgatcgcga ccgtactggc tcatgaaatc
    38821 ggccattgca ttggtttccg ccacacggat tatttcaacc gttcgataag ttgcggagga
    38881 tccgccttag gcgaaagcag cagtggaggc gccattcaca tcccgggtac tcctacggat
    38941 gccacccagg ccgcccagtc ctggatgctt tcctgcatcg gttcaggcca tgaccgcccc
    39001 ttcaatgccg acgataaagt ggcactggat tatctttatt gatatcaaag ccaataaatt
    39061 ttacaagggc tgcacctttc tgcagccctt ttttattttt ctgtcgggga gagcctgtga
    39121 attgaaaggg gttgggagaa tggaagcccc atgcagtttg tgctgcaacg gttaaggttg
    39181 ccttcccttt attttgagca tgcccgcgtt gatggccacg attaccgtgc tcaggctcat
    39241 caataccgca ccggctgcgg gactcaataa gaatccctgg ggatacagga tccctgcagc
    39301 caatggcagg gcgacaatat tataccccgt tgcccagacc aggttttgaa tcattttccg
    39361 gtaggtggct ttgccaaaac ggatcaggga gaccacatcc gcgggattgc tgttgacgag
    39421 gatgatgtcc gccgtttcag cagcaatatc agaacccgaa ccgactgcaa taccgacctg
    39481 ggcctgggca agcgcagggg catcattgac cccatctccc gtcatcgcca caaattcccc
    39541 ctcctgctga agttctttga ttttgtcgag tttttgatgc ggtaatactt ctgcaaaata
    39601 gtgttccata ttcagggttt gggcaatggc ggcagcgacc tcgaggcggt ccccggtcag
    39661 caaaacgggt tttatgtttt cccggcgcag gtcccggatg gcgccggcag actccggacg
    39721 aagggtatcg gaaagtgaaa taaaaccgga tacttcatcc cctcccagga caaataccac
    39781 ggtctcgctc aaatctgcgg tataatccgg gggaaaagga atttgatttt ccttaaggta
    39841 acccgggctt accactttga tgtcttttcc ttccacgtgg gcttcgatgc ctttgcccgt
    39901 gatggcatta aacttttgta ccgacggcac agggatgccc atgcttttgg ctttgtccat
    39961 gatgccggaa gcaatcggat gctctgaatt ttgttcgagg gctgcggcca gacggattaa
    40021 ttcatactcg tttaactccg ggagcacagg caccacacgt gaaacttcga atcggcccag
    40081 ggtcagggtt cccgttttat caaaaaccag ggtgcttatt tttcgcgcgt tttcaaaggc
    40141 cgtccggttt ttaatcaata aaccatgttt tgcggaaagg gcggtggatt tggccaccac
    40201 cagaggtacg gcaaggccca gcgcgtgggg acaacaaatc acaatcacgg tgaccatcct
    40261 ttccatggca aaagcaaggc cgtttcctga gaaataccat accgcaaaag tgatcagccc
    40321 cgcggcgatg gcgatcaggg tgagccagcg cgctgcacga tcggccagca attgggtttt
    40381 tgatttggat tgccgggcat cctgtaccag tttgaccacc cgggccaggt aagagtcctt
    40441 tgccccgtgc gataccctga tttttatcgc cccgtttcca ttgatagatc ctgcaatgac
    40501 ctggtctccg tgctttttgg ctacgggtcg ggattccccg gtgagcatgg attcatccac
    40561 atacgtttcc ccttccgtaa tcaggccgtc ggcagcaact ttttcaccgg gtttgacgag
    40621 aatgatatcg tttgttttga gactggtggt ttggacctcc atgatatggt ttccgtgcac
    40681 cagatgggca tcatccggca tcagccggac cagcaagtcc agttccctgg acgccccggc
    40741 caccgatttc atttctatcc aatggcccag gagcatgatc aggataaggg tggccagttc
    40801 ccagaaaaaa tccatccctt gtaaacccag caccgtggcg gaggaataga tgtaggctac
    40861 cgtgatggca aaaccgatca gggtcatcat cccgggctgc caggatttca tttcatcaag
    40921 ccatcccctc aaaaaaggcc agcctccata aaaaaacact acagaagata ataaccagag
    40981 caaacctttt gagcctgcaa aaaccaaatg gaaacccagc cagtgctgga tcatttccga
    41041 caagagcagg atgggcaggg tcagcagaag gacggcccag gcacgttttt tgaaatcgtc
    41101 catcatcatg gaatggtggt cgtggccggc atgacccatg ggtggattgg gattgccgtg
    41161 gaccggaggg gtttcttgat gaagcggttg atggtggtgt ttatgttcca tatctcaacg
    41221 ttgaatggtt aaattaaacg gaatccgaat tttttcccag acaatggcga tgtcgatctt
    41281 tccatcctga tgtgtttcca tgaaatactg gagacgctct gtatgttctg tttttctcgg
    41341 cttgacccgg attcttatca aatcatcttt tgggtcatat tcggaggtca ggtgttgttc
    41401 ccagtgttta ttcagaataa cggtccattc tttcttgccg ggaatggaaa atatggcata
    41461 ttcaccggcg ggtagacgtt tgccacccat cacgaaatca aaagggatgc tgagtttagt
    41521 ggcattatgt gcaccggtga cccaaacttc accaaacgga accaaacctc cccaaatgat
    41581 tctgcgacga actccgggtg aatggtaatc aatggtgatg ctgtcctgct cgaggcgcag
    41641 gatagccctg gcagggatgc ttttcctggt ggtgtccgat ttctgattcg ggttataatt
    41701 gatttcaccg gactgacccc aacctatgcc tgaaagacaa caaaaaacaa aaatctgctg
    41761 gaatttgatc atgattataa aattttaatt attggtcgtg agtgtgcgaa gccatgggat
    41821 ccgctccttt tttgaaaagg tattcactgt ttaataaata ggcgccccgg gtcaccactt
    41881 cttctccggg agctattccg gacagaattt ccagattacc ttcagattcc cggccttcct
    41941 gcaccattcg gggtctgaat ttattttttc cacttcgaac ccagaccgta gaacccctgc
    42001 tgtctttaag caccgcctcc aggggtaagg tcaggacctg atccaggggg cttgtgagca
    42061 agatctgggc ctgcatgccg ggtttcaaag tatgatccgg attgggaatg gatacgcgca
    42121 gcagaaggat acgactgtct gctttgagct ccggagtcat gaactcaata gttccggaaa
    42181 catccctgtt gtataaatcg gggaagcgga ctaaggcttt gctcttcaaa ttcacatcag
    42241 ccatttgcga ggaatatatt tgcgcttcag cccacaaggt ggagaggtca gccaatttga
    42301 cgatcaggct tccagccatg gcgtaatccc cttccagcac gttgagttcg gtaatatatc
    42361 cgctggatgg actgaggaag gtcgtcgcca gggggtttga taaaatcgtc cattctgaaa
    42421 tttgacgttc actcatgccc caaagaatca atttgttttt tgcattctga atcaggtgat
    42481 ccatgtttat ctggccgtcc ttcgggtagg tgtttttatt ttcaagggcc tggagatatt
    42541 cgagtctggc ggtattcagt tcttcgctgt aaagttcgta caatggtttt ccggacacca
    42601 catagtctcc cagattttta taatacagct tttcaattct gcccatgatc cttgaactga
    42661 ctgaattgat ttttctttgg tcaaaattca atgttgcgtt taataatatc tttttaccga
    42721 tcctggaggt tgccagcgta tcggtttgaa tattacccag gaggatttgc tggtcactga
    42781 gttccaattc atcccggcgg ttccccttgc tgatttttac cggggtcaat tccattttac
    42841 atattggaca ttttccaggt ttgtattcca cgacctgagg atccatggaa caggtataat
    42901 aaagctcggg gtctttcacg ggcccggttt tttggttaca ggccagcagg aaaagaagga
    42961 ttacggaaaa aataagggat ttcatggctt gtttttgacg gtgatgaaac tctcgctgtc
    43021 caataagtac tgggcattta tggcgaaccg gtcatgtatc gaaagaccat ccttgaattc
    43081 aaccaggtcc tccattacta aaccggtctg caccggaata gctttaaagc cgccttcttt
    43141 tttaagaaaa accactttct ggaggcccga ggagagtact gctgatttag gtaaccagta
    43201 agaggttttt gaaggccctg tgatttttgc agttatgcga ctgccaatgg gcaattgccc
    43261 gtaacgatgg tccaaataga tcctggcggt aagggtttta cttcccttct ggtaaaaggg
    43321 ttctataaaa ttgatttttc cgcggaggat ggtttgaggt gaggtttctg cccggaggat
    43381 ggcctcattt cctattttaa tccatccctg gttttctccg tggatattaa ttatagccca
    43441 tgtacgtttg ggatcgtaaa cactcaacac aggctggcct ttttggacat acatcccttc
    43501 tttcagggtt aagggctcct cctccccctc tttattggcc atcgcgccct ctgaccggcc
    43561 ctccatcctt tcggtttcga tgacgtaacc gtttcgtttg ctgtacacgc caacggtaag
    43621 ggagggttca cctgacttga tgacttcttc catctgggat gtttgcattc ccaacaggat
    43681 taatttttgt tttgccgcac ggaccaaact ttcattgtcc ggatcctgcc gcaagttaaa
    43741 aagcagattt tgttgagcag tgaccagctc ggggctgtag acatccatga tccgctgtcc
    43801 ttcatggatt ttctggaacc tataccggat atataatttt tctatacggc cttctgtatt
    43861 ggatgaaatc cggtacactt cccgggtatc gtagtcaatc acgccggtag cttctccaat
    43921 cagggagact tcctttttta gaagggtagt atcccggact gtcgaaatga tgaattcatg
    43981 agtgggtttt aacaaatctt ccaaacgtgg aatggaagga tcttgttcgg tttgattttt
    44041 ttgcaccagt ttcatattgc agatcggaca actgccgggt tcatcctcca tgatttcagg
    44101 atgcatagga caagtgaaga cgggcggatg acctttatgc agagcaaagt ttgtttgcac
    44161 caggtccatt ccgcatttgg gacattgccc gggctccggg ctatagaagg aatcctccgg
    44221 catgggacaa gtataggtta gcggaatttc ctgaaccgaa ggtcgatctg gctgaagctt
    44281 gcaggctata acatgcagca tgacgaacag aaaaagacag ccttttaaaa ttttcatttt
    44341 tgttgtatta accgttccag ttcaacctga gaccggaggg cttggtttaa caaatcgaag
    44401 tattccagtt gcttcatatt tagggcttcc caggcgtcgt acaacatgaa tagttcctcg
    44461 gtattttgtt cgtaacccaa ttgcatggtt tgtaaattgt ttttcagagc cggaataatc
    44521 gtttcttcat atcccgacag ttgcctgatt ttaagttgca actcattgcg catggcatag
    44581 gccatcccgc tgtattcgtt cgccagcatt tcttttttgg cttcataaga catggcctgc
    44641 catttgagac tttctatatt agcgcgggtc attttggaag accattttac cattggaatg
    44701 ctgaccatgc ccataaggtt aaataaaagg ggctggcccc cgaatccaaa catatgttca
    44761 taacgaaggc caaactgggg tctgagggtc tgcttctccg catcctgccg aagccagttc
    44821 aggcgaatgc gttggtccag cgctctgata tcggaacggt tggaaaagaa aagggcctgg
    44881 tcaaaaaccc aaagactaaa atcattcaaa tgaaacaaag tgtctaattc caagggtatg
    44941 gtgggatttc ggcccatcag cgtattgatg cggaagtgtt tatcccggat ctcgctttca
    45001 atcatccatt gcatgttttt aagattgccc agggctgctt tggctttgta ataggctccg
    45061 attttgccca atccgaattg atagcggctt tctgcattct gcaccatgaa ttcaaggatc
    45121 tgccggtttg aaccaagcac ggcgagtttt ttatttaaaa tcatggtttc aaaaaataaa
    45181 cttttggcgt cgtgtaccaa ttcatgcaac acgttgtttc tttggtcata ttctgcagag
    45241 gacatggcct tcatatatcg ttcctctgca tccagtttct ttttattggg caacatttgt
    45301 tcgcctgaaa tcatgatgga tcccatcccg gtcatttctc cttccctgct ccatcgattg
    45361 atgttgtagg gggtcataaa tgacccgatg gcaaactgtg gaggcatcca actgcgggcc
    45421 ccttcagccg cttcatccat ggatctgatc tcgcgggtgt acatttttac aaccgggtga
    45481 ttttgacgaa tggaatccag gatccgatcc agggtgtata tttcctgggc tgaaagagcc
    45541 cgaaggaaga ggaaactcag tacaggaatg agggttaaac gggttttcat gagaagctgc
    45601 atttatttat ccaaggatgg tttgttaggt taaatcttca ggttcctgaa gatctttttt
    45661 caaatcggca tttcggatca acgcatacag gaccggcaag acgatcaaaa caaacaaagt
    45721 ggaagtgatc aatccaaata caaaggggta ggcaatgggt ttcatgacat cacttccggt
    45781 tcccgttgcc aacaagacgg gcaacaaacc caggatatcc acaatgaccg tcatcaattt
    45841 gggcctgacc cgcaatacgg cgccgtcgta gatggcctct tccagggcat gggggttgag
    45901 tttaagattt tgttcacgga aagtctttat ttttttctcc caggaatggt tgagatacac
    45961 cagcatcagc acccctgttt caacggcaat cccaaacagg gcgatgaaac cgacagcaac
    46021 ggcaacggaa aaattaatgt caaaataata aagggaataa accccgccca ccagcgcaac
    46081 cggaatggcg gataatacga cgagcacttc cctgaaggac cggaaagtaa aatacaaaat
    46141 gatgcaaata atggccaata cgacaggcat tattagtttc agcctttgtt cagcacggat
    46201 ttgattttca tattgcccgc tccactgaag gaaatatcct ttgggaagct tattcatggc
    46261 ctctgccaat ttgtttttgg cttcgtcaac cgtacttcca aggtccctgc ctctgacatt
    46321 gaacaacagt gttccccgca acatggcgtt ttcggaggca atcatgggag ggccttccgt
    46381 gagttctata tccgctaagg ttccaagcgg gactgggcca aaatccatgg tcattaccgg
    46441 tatttgtttc attttttcaa tggaattccg gaaatcctga cccaggcgca ggttaattga
    46501 aaagcgctgg cggccttcaa ccgtttgaga cagcgtcata ccacccaggg cactttcaat
    46561 gaacatgttg acatcgtcga tagtcagacc gtacttgccc aattcttcgc gtttgatccg
    46621 gatatccaga tatttgcctc cgatgatggg ttccacatac agatctttaa ctccatcgag
    46681 gccttccagg gtttttttaa acaattgggc caattggttt atggaatcga ggtgctggcc
    46741 gaaaattttt attcctacat ctgtgcggat tccggtggaa agcatattta tgcggttgat
    46801 gatgggttga gtccagccat tgatgacccc gggtatttgc agttttgcgt tcaattcatt
    46861 gatgatgtca tttttagtga ttccttttcg ccacagcgcc tggggtttta aaaggacaat
    46921 cgtttcaatc atggaaatcg gagcattatc cgttgccgtg ctggctcttc cggcttttcc
    46981 caggacgttt tgtacttccg gcaccgtgag catgagttta tcctggacct gcatgatccg
    47041 ttttatctcc gcattggaga tgtccggtaa ggtgacgggc atgaacagaa gagagccctc
    47101 gtccagaggg ggcataaatt cacggccgag tcttaatagg taagggatgc tgcccgcgac
    47161 cagggcgata cagaaaaaca gcgttagttt tttccatttc aggcaccagc gaatcacggg
    47221 tgagtacaaa gagataataa aacgggatat gggatgcttg ttttctgaat acacctttcc
    47281 tttcaacagg acgaccatca ggaccggcac gaggaagacg gcgaccaggg ccgacccggc
    47341 catggcaaat gtttttgtcc aaaccagggg ggaaaacagt ttatgttcct gaccggataa
    47401 aaacaaaatc gggctaaatg aaaccagggt caccagtatt gaactgacta ctgctttcgc
    47461 tatcagcttg gaagattcct ctacccgtcg catccgttct tttggatcca gcatagacat
    47521 tattgttcta attctccgct taatgatttc atggtatttt caaccatgac gattccggca
    47581 tcgaccaagt ccccgacggc aagggctaca cctgccagag acatgatgtt ggaagtaatg
    47641 ccaaaccact tcatcaggat gaaggcgatc aggacagaca taggaatggt gatcaccaca
    47701 atcaatgcgc tgcgcagatg gagcagaaag acaaacagta ccagggagac gaccaggatt
    47761 tcttccagca ccgcctcctt caaggttgcg atggtcgcct gaatcaactc cgaacgatcg
    47821 taagccattt tgaaccgaat cccctggggt aatccttttt cgacttcctg catgcgtgat
    47881 tttaaccggg caatcacatc gtttgcattt tctccatacc gcatcaccac gatccccccg
    47941 actttttctc cttctccatt ttcctcgact atacccagtc tcaattcacc ccccatttga
    48001 acatctgcaa ggtcctttat cctgaccgga atggaattga tatgggttac cgcgatgtct
    48061 tcaacttgtt tgatgttctg tatataaccc agaccccgga tgatatagcc catatccgcc
    48121 tgttcgaatt ttcgtccgcc gacatcttta ttgtttgctt ccagggcgcg gacaacatcc
    48181 attatcggaa tcctgtaata ctggagtttc aatggattta ccacgacctg atattgtttt
    48241 tgaaagcctc cgaaggaagc cacttcactc accccctcca cggtttgcaa agcaaatcgg
    48301 atgtaccaat cctgcaaggc acgaagttcg cccagatcat gccctggcgc atccagggtg
    48361 taccaataaa catggcccag gccggttcca tcgggcccca gcgtgggaac cacccctttg
    48421 ggcaagagtt gttgggctga atttaatctc tccaataccc gggttctggc ccaataaata
    48481 tcgacctggt cttcaaagat tacaaaaacg aaactcatcc cgaacatgct gttggccctg
    48541 atgtttttga ctttaggcaa gccctgtagg ttgctgacca acggataggt tacctggtcc
    48601 tccatgatct ggggtccccg tcccatccat tctgtgaaaa tgatcacctg gttttcagaa
    48661 agatccggaa tggcatccac cggggtggtt ctgaccgaat aaaatccata ggccgcgatg
    48721 atggcggcaa caagcaagac gatgatccgg tttcggagcg cgccttctat gagtttatca
    48781 atcattggta attatataag ttaatgattt ttacaaaatg caacccaatt gatgcgtacg
    48841 tatttaaagc ggttatatca ggattttttt ttgagtttta cggattgttt tttaaaagcc
    48901 agttcagctt atacatttta tggaaagtat cgtaatttat aagctctgca tcggaattaa
    48961 aatgagcatc cagggcattg tgaaaagcat ggttggttgg aaaggctttt ttcaggtttt
    49021 ctttagtcag atccttcagc acatctgacg atgcggtggt gaaataatat ttaggagcat
    49081 atttatccac ctccttggcc gaatgtgcct tatgcatgta cttgtacagc agtatggatt
    49141 ctcccgtgtt gatgaccgag tattctttcg atgaagaagc gattttagca gtaacttccc
    49201 ctttttgagt agaagaacaa ctttgaataa ccaataacag gctgcttgca cagaataata
    49261 cactgtgtaa ataattcgat gtctttttca ttttaaaaaa attaaataag atttaaaata
    49321 atcaataagt catagtaatc ccggcacccc aacccatatc cttgtcataa tgagtggaca
    49381 aagccaggtt tttagtcaat atatactgca ggccggccat atattcccgg tccgaattgt
    49441 acatcagggc cattctgaat cttttactta agggaatatc ttccctcatg agctgaaggc
    49501 ggagcctgcc gtccatatca acacgggcat cggccttgaa aagcagggga agcgtgtacc
    49561 ggaggccaag acataaagct ccacgctgat ctttggtatt gacctggcca aaccagtttt
    49621 tttcacgttc atccgacttc cggtatctcc agtcaaaacc caggtaaggc attagccatt
    49681 gcatttttcc gagataccgg ccgatgtggg tttccgtttc gtagccgtgg tcgtcgtggt
    49741 aacccaggcg ccattccgct cccaggctcc atcgggtgtt ttgcaacatc atttccccgt
    49801 cattgccgtt gctggcaaaa tcattttgaa acataaaatg aaactcccgg tcgtcgcgat
    49861 acaatttttt gagagaggcg gcagggtcgg gaatctcggg attgggcgga gaatcttcat
    49921 aactgaatat tctgcccata ccgctcatca tatgatataa aatatggcaa tggaaaaacc
    49981 aatcattggt ttcagtcgct gcaaattcaa tggtatcggt ttccatggga aagatatcca
    50041 ctacgttttt aaggggtgca taatcgccct gaccgttcaa caacctgaaa aaatgcccgt
    50101 gcaaatgcat gggatggcgc atcatggacc cgttgtacag gatgatcctg atgttttcgc
    50161 ctttgcggat gaggacttta tcggtttccg aaactgtttt attgtcgaga ctccagacat
    50221 accggttcat gttgccgctc aattcaaaac gcatggtttt cacaggcgcc tggggcagtg
    50281 ttgttttaaa cggggagcgc aactgggcat aattaagggt tacgatttcc ggcgtggcgt
    50341 cgttttggtt ttgttccggc tggccgtgct gatgctcttg ttttttcctg tcctttcccg
    50401 ggccagtgat ttcggggtac atgaccacat tcatatccat ttgctgcagg ctcatattca
    50461 tgcccatatc gtcaaggtca ccgttcattt tcatcatctc attcatcatc ttcatgcctt
    50521 cgaaatattt taaccggggt aggggggtgg ccggttgttt ggtccccttc cccaaccaga
    50581 gagaggtcga ttttacccgg tcttcggagg tggctaaaaa ctcatgggca aggcctgatt
    50641 ccggaacggt cacgaccaca tcataagttt ctgcgacggc aatgatcatc cggtcaacct
    50701 ccaccggtac gacatccggc ccatcgtttg cgatgatcgt tattttccct cccgcatagt
    50761 tcagccagaa ataggtggaa gaactcccgt tgatgatcct gagccggatt ttatccccgg
    50821 ccttaaattc cgcagcttcc aattccgctt gcccattgct caggaaacgg tcataataca
    50881 catcactcac gtccatggcc agcatccgct tccattcatt gaccagtttt gtcttgaaat
    50941 ggccttcgcg gatggcttcg gcataactct gggtggcacc ttttttgatg gcataccagt
    51001 cggtggcatt atgcagttta cggtgaacct caaaaggctt ttcatcgctc cagtcgctga
    51061 gcaccagggc ataatccggt atttgatctt cctccctcca ggtcgggtcc ccggattttt
    51121 tacggatgat aaaggccccg tacatgccac tttgttcctg gagtttggta tgactgtggt
    51181 accagtaagt gccgttttgt atcagcctga aggtatagag gtggctttgt ccgggcagta
    51241 tcggacgggt tgtgagataa ggtactccgt catattggtt aggcaggatc aggccgtgcc
    51301 agtgaatgga ggtttcttct ttgagccggt tgtggacata aatgaccgcg ctgtcccctt
    51361 cggtaaattc aagagtgggc attgggatgg ttccgttgac ggcgatggcc ttcctcgttt
    51421 tgccgctata gttgaccagg gtatccgtga tgaataagtc gtagcgtacg atttttgccc
    51481 cggctgcaaa ccccagaacc ggcaatagaa aacttatcaa cggcaacccg attttaccaa
    51541 aatacaggtg aaagatcgaa gatctcaagc cgtgcacaga gcattggaat ttcataaaca
    51601 gcagtggatt tgaaataaaa aacgagcccc tggtttattg ctgaatggtt tcaacggtgc
    51661 gcccgcagct gagcatcgaa gccccgtaat aggggttttt tacggggttt tccttactca
    51721 gccaatgggc tcctttgccc ccattggcca tggggcaatg ctgataataa acggcttcca
    51781 ggggtttcga ggatttaatc agggagtaga tattttcaga aagccggata aaaatttcgc
    51841 gttgccgggt gatttttttt gtgccggcca ggtctttggc atcctgcttc agttcctttt
    51901 ccaccttcat ccaggccagg tgttcttcat ggcccagttt attcatttcc attttgtcga
    51961 gcgcggcgac caattggcct ccatggaggg atgcgttttc ggcctcggat tttaccaggg
    52021 cgtcttttat ttggaaataa gattggaaga ggtctttaaa taattcactt ccggcttgtg
    52081 cacgggacct gaaagcggtt ccaatgaata ttacagccag gatcaggata ggattgatgg
    52141 ttttcataat aaaaaagata ggatttagaa aaataaatta ttgaacatcc ggtgaggaac
    52201 cggagtcaaa cctgggaagg gacaggctaa atcctgaaaa cgcagttgcg gatataaacc
    52261 gcgacggtat ttaagggagg aggtaccgcg ttcaccgggg ccggcaaggc gcggtcctgg
    52321 aagtgtaaat aaacgggttc gggatgcagc gcaggcagta ccgtttcggc cagggccagg
    52381 ttcagggaga aggaagaacc gagtgttttt tggtctacgc tggttttcag tacatggtgt
    52441 tcatccctgc agcagccctt gtcctttcct tcggacttat tcatgccgca tttgccgcag
    52501 atatccggga gttcataacc caggctccaa ccggccagcc gccccatgca ataatgggta
    52561 tgtaaggtca ggcccgagga ggttccaagg tagaggatca ataaaaaact ggataatagt
    52621 tttttcatga ctgcattaca aagataagcc tgatttgtag tcaaaaggtt ataggattct
    52681 gaacggcttt tataataaaa ttcaagggag ctaagctcct cccgaacatt cccgcacaaa
    52741 aagggcctgc aggcatttaa atcatgtata ggagcatgat caggctcatt ccaatcatca
    52801 gggcgtcctc cgcgatggtc actgtgctca tgggtaaatc aaaaaccttg cccaggcagg
    52861 cgcattgaat gacccttttg ttcagcacgg tttgaagcac acccaccagg ctcaggctca
    52921 tcacggcaaa ggtgatcgtg ttggttacca gggggaacca tccgccgagg tataaaagac
    52981 caaggcccaa ttcgacgaag gcatatacat atccccaggg agcccatctt ttcgccagga
    53041 tatcgtagcg ggcgtaactt tccgcaaatc ctttcaggtc aagcagtttg aaaaacgaaa
    53101 aaaccaggaa aaagccggcc ataaaatgat tcatccagcg catccagcca aaggaccccc
    53161 ggtacgcctc gataaaaagg gtaagcccca ggaggttggc gaaaaccagc aggatgggtt
    53221 tgtaggtctg gatccaggat ttttccggag tagcctcatg agcggatggg tggggggtga
    53281 ttttgtattt attggggccc agggcttgct gtagttcaga caacggaatg gctttttcca
    53341 attccagttt tgcctgttgc gctgttttgg atacttcagc cgattttaca tggggcaggg
    53401 actgcaaggc atttttgaca gctagttcac aggatccgca ggtcattccg gtgacggtat
    53461 aagtttgtgt catgaataat agtttttatg atacaaaatt gagaggaact gatccgtagg
    53521 ccgttatatt attccgtcaa agagttgtaa ttaattgttg atcagtaggg agcattgttg
    53581 gggggtgact acaaagggtt cccacgacgg cacagcgaag cgaagggcac aaaaagttta
    53641 gtccacgacg acatggtgat ggttccccac aacggcacaa tgagcataaa agggttcctc
    53701 aagtcaaggc gcatttaaga gcaaggattg aattccccca gtttattccg gtctttgctc
    53761 aatggattat gttagaaagg ttaggcttct gcgaagcctt gatcagtatt gtggttggac
    53821 ttagaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttag aaaggttagg
    53941 cttctgcgaa gccttgatca gtattgtggt tggacttaga agggttgggc ttctgcgaag
    54001 ccttgatcag tgttgtggtt ggacttagaa gggttgggct tctgcgaagc cttgaccagt
    54061 gttgtggttg gacttagaaa ggttgtgctt cgaagccggg atgatggatt gcaaatttct
    54121 aagtgttgat tataaaggta agcgggcaaa ttatgaggtg ggcttcaggt gtcccgcctg
    54181 aaacttttta gcaaaatgcc tggatgaaat aaaggatatg aaagttgcca aactttttgt
    54241 ttcccttcct aacgttttgc tctgtttaag gcctgcccga ctgtatttga caagaaggcg
    54301 gttaggcggg cgaatgttga ctattgacta ttgactattg actattgacc attgactatt
    54361 gaccattgac tattgaccac tgacgatcac cttatcaatg gacttcctta atccccctat
    54421 ttttttaaaa gcggtgggtg tgagcccggt aaatttttta aactgggaag ataaatgagc
    54481 cacgctgctg tatcccagtt tccaggaaat ttcgctgagc gagagttcat cgtagacgag
    54541 cagttccttt gccttttcta ttttttgcag gataaagaat tgttcgatgg tgatgccttc
    54601 aaccccggaa aacaagcggg atacataaga atagtcttta tgcaaagcct tgcccaggaa
    54661 ttcactgaga ctgaaatgct cttcgatggc cgcttgctgc accaggccga tgagcaggct
    54721 ttttattttt tcaatttgtt tttgtttttg gttgtccagt acttcaaagc caagggccct
    54781 caggcgttgc cccaggtttt gtaattgttg cgcagtgggt tcatgggacc attccacttc
    54841 accaaggctg atggaggccg gatgcaggcc cagcttttcc agttcctggt tgacggccat
    54901 cacacagcgg ttgcacacca tgtttttgat gtaaagacgc atgattaggt ttaaaaatag
    54961 gacgaaaatc caaaactgaa tcctcccgtc cttgcaggag gattccccag caggtcccat
    55021 tgccgctgaa tccggtaatt taaccggatt acggtttggc cggatggccg gaaactcagg
    55081 ccgggtacca ggctccagag atgatcggca atcgggtcac cggttgaaac gaacttattt
    55141 ctattccaat cgacgtattc cagacgaacc gccaggttca tcacggcatc ctcccattcg
    55201 aggatccgct ttttcacaag gggttgaacc agatccacga aaccgcctcg ttgtttgttg
    55261 ccgtattgtt cggtataggt tggcgggaca ttcaccctga tccaggccca ttctccggtg
    55321 ataaaagtat tcaggaccgg caaggtgccg ttgaaatcga tggcaaagac atccaccctt
    55381 cttttttcat ccagcggcaa gccatcctcc tggtatttgt tgtacacccc gcccatataa
    55441 gaaagcccga cttcacccag ttggttatgc cttgcggcgg ttttcagggt aaaaaggggc
    55501 aagccgctgt ggatatcttc aaaccggtcc gcattttttt tggcctctgc cagggaagtc
    55561 ctgttcaagc cattgtcaat gatggactgg tcaaaattac cggagaggta agcctcataa
    55621 ccgaacatcc agtccctttt attgtatttc ccgtacaggc caaaacctgc attgctccag
    55681 gtcgccggaa ggagttgggt tgctgaaatg ggccggtcta tgaattccca tttgggcccg
    55741 tcgtgattct gattaaaggc accgataggg ttcaggatca cccctcccct caggttgagc
    55801 aggggaaacc attcaaggtc cagggctgca aattcaagag ctatttcttt ggtgccgtct
    55861 tcaaattcga gttcactgag aaattttaat cgccgggcaa tggttgatga aacaaacaag
    55921 gtcatccgcc tgaactgaaa ctgatggcct tcggatatcc cgtccgtgcc caggtgttgc
    55981 cagttggctt ccaggtaacc gcccagcgaa accggcagtt taccggcgct ggtaaaagga
    56041 cggttgtata ccgcatccat gttcagcatt tgttttgaac tgtctccggg gatctttaat
    56101 aaatccggat ctatttgggc atgagcggga agccaatagg tccaaaaaac gaggaatccc
    56161 agtaaaggac gcgtcatcat ttttttaaag agattttaag ccggtcattt tcaatccgga
    56221 ccggaaaggt tcggagattt tcgtccgcgg gtccattacg gatccggccc gaagaatcaa
    56281 attcactgcc atgcgccggg cattgcaggc ggtccccgaa gacctgcagt tcggtgccct
    56341 ggtgggtgca ccgcatccaa agcgcccggt attcctgccc tgagacccgg aaaacacaaa
    56401 ccgggtattt caggatttca tggtaaacga tgacggaatc cctgaaaacc gtttttcctt
    56461 cttttccgga ttcaaaacga tccaggggca ggaccaggtc atctgcatca atatcccccc
    56521 ttacccgccg gatacttcca caggcggaca aacctccaag gccggccagg caggccaatc
    56581 cacaggtctt tatgaaggta ttccggtcca ttcttcaaag ggattccaga aaccggatca
    56641 gggcttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    56701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga aggtattccg
    56761 gtccattctt caaagggatt ccagaaaccg gatcagggct tcccggtctt caacattcag
    56821 ttgattgaat ttattccggc tggtttcggc ttcaccgccg tgcaacaaaa tagcctcttc
    56881 aatgctacgg gcacgtccat catgcaggag gaagtactga cctccctggg aatccggtga
    56941 aaggccaagg ccccaaaggg gtggagtgcg ccattcatat gttttggcgt tgccttcggt
    57001 ataaccatca tccaaagcat tgcccatatc gtgaagcaaa aggtcggtgt acggatgaaa
    57061 ggttttatca gacagggcgg caatgggaga aggccctgtc cggagggatt ctttgtggca
    57121 tttttcacaa ccggttgccg taaaaagcga tttaccggct ttgatcttcg ggtcatccgg
    57181 tttcctttga atgggagctt tcagggcctg gagataaaaa acgacctggt ggatgttgag
    57241 gtccgaaatt tccggttcaa tgtccagacc ggtgtatata tccgtgggtg cgaaactgga
    57301 ggatatgccg atatcctggt ggtaggcgtt gaccgtctga tgcataagat cgaaagcgga
    57361 ggcttttttg ccaaaccggt gaatatactt tccattccgg ctgatcttat cgctgcctgg
    57421 ccgaatataa gccggcaaaa cggcccagtt gggtacgccg gagatgccat cccagttggc
    57481 atcttccgga tcggccatgg ccagcaggtc ctcatccggt accagttcga ggtatcccag
    57541 gccggtgttg gccggaggta taaaggtgga gtggcttgcg ccggggggta aggattccgg
    57601 ggcataacct ggaatggacc ggtgttgcag ctggggacct ccggcatgga ggtaccggtt
    57661 gcccgtgctg tcggtctgtc caaaacggac cagggagctg aacgggtggc ccttgccgtc
    57721 ccccgcatgg caggagccgc agctggtagc tacgaataag gggcccaatc cggtcgggga
    57781 ggtgaaaact tcatcgttaa aggcccggtc ccctgccagg aaacggctgt tttgttcaaa
    57841 actcaggccc tccaccggac cgtccaggag ttcgtcttcg gcaggagcgg cgggttccag
    57901 gctgctgcag gaaattaaaa gagctaaact tggggttata cagggtccga tccactgaac
    57961 gatccggtgc attattttat tcgtaaaagt ttaaagttag acaaatctaa caattatttt
    58021 tatacttcca taatacattt tttatgcatc cccggaaagc ctacctttaa gtcatgcatt
    58081 cccataccga agaaaattac ctcaaggcct tgtttaactt ggcgaatgaa aaaggcgagg
    58141 tgagtgtgaa tgacctgagc aagatcctgg ccatcaagat gcccacggta aacagcatgg
    58201 tcaaaaaact ggccggcaaa aagctggttc actatgagag ttacaaaccg ctccgcctca
    58261 cggaaaaagg caagaaggaa gccggcctga tcatccgtaa gcaccggctg accgagatgt
    58321 tcctggttca gaaaatgggc ttcggctggg aacaagtgca tgaaatagcg gaacaaatcg
    58381 agcatatcca ttccccctat ttttttgata aaatggatga gatgctgggt tatccgaaaa
    58441 tcgacccgca tgggtccccc atccccgata aaaccggaaa agtggattgg atcagatatg
    58501 agaagctgag tgattgcgga gccggtgaaa ccgtccggct gggcgcggta attcattcga
    58561 ccgaggagtt cctgaaattt ctaaacagcc ggaaactcca tctcggttta aaaattaaaa
    58621 tcaaatcggt ggaatctttc gatgggagca tggtggtgtc ttatgggaaa aactccacgg
    58681 agacgctaag cctgacggtc agtgagcggt tgctggtgga gaaaatcggg tgagagtagt
    58741 gcgcacggag ccaaggagaa cacggaggtt gattttgtca agttctggat ttcgttggat
    58801 aacattttga gacttcaaat tcattaaaaa gataatctcc gtgttctcct ttcctccgtg
    58861 cgaggtcaaa taggcggggc cgcctgaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58981 nnnnnnnncg gagatcaatc attgcaatcc cggtataaat aaaaaaatct ccgtgttctc
    59041 ctttcctccg tgcgaggtca aataggcggg gccgcctgaa ggcgtcccgg cattaatctc
    59101 acggagaaca cggagcgcac ggagatcaat cattgcaatc ccggtataaa taaaaaaatc
    59161 tccgtgttct ccttacctcc gtgcgaggtc aaagtaggtg ggggccgcca aaggcgtccc
    59221 gttattattc acaaggagtg cacgaagagc acggagatca atcattccaa tgctggtata
    59281 aaataatctc cgtgttctcc cttactccgt gcgaacctta ccccggtctc cgtgatattt
    59341 attgttttac ctccaaatcg atatcacaga aaaaaaggcg ctccccgaaa tctggaaccc
    59401 tcaccaaacg ggctccctgc cggtcaatca atcctgactg ggcattccct tttttatctc
    59461 cataagccgc attcacttcc actatgggta tcccgagatg gagcgcccaa agctcggcgg
    59521 aggattcatg gaaaggacgg tatctctggc tggtacccga attgatggca tggaaaatga
    59581 cctgggctcc catttctttg agtttcaggg gcaggtatgg gttgagaatc gtggtatatc
    59641 cgggagtagc ccagagatcg ttgcaaacca gggcgccaaa gcgtaattgg ttccagttaa
    59701 aggtttgtaa agtaccggca gtgtattgtg ccatctcacc ggtaccgggt ttatccaccg
    59761 ggctgcatac caggatcttg gaatgggcgc ccaggaaggt tccctccggg gaatagatcc
    59821 ggacctggtt gtaacagagt ccttcttcat ctttaaagca agtgcccagc atcaggccga
    59881 gattcaattc ccgcgccagg cccaggactt catttaaagc cggttccaat tccttttggt
    59941 taaaagtcga cctgtaaccg gataaggaac cttcgggagt gatcagaaaa cttgctttgc
    60001 cggcggccgc ctcccggata gcggtaagaa tacggttttt attcgttgag atgtcatcgc
    60061 tgacgttcat ctgcaagccg gccactctga gcccgcctga atcttgtgct gaagacaggg
    60121 taaaacaacc cagcaggcag caaaacagga ggaaagtttg gtatttcttt ttcatagata
    60181 attacaaatt acggcaaatg gttaggaaat aattatgctt tgttttccat tgatttacag
    60241 gcccggtcta aaccaggccc agagccaatc cggctgccaa caaaatcaac atcaccgtca
    60301 ccagcacctg tacctggccc aaggtcacct ttttgaggag caggttgccc aggaccgctc
    60361 ccaggaaagc ggaaaaggaa gccagggcca gcaggattga attttccatg agttccgagt
    60421 gcatgtattt ctccgaataa acaaaaagcc tggagacatc caccaggcag gcgatcaaca
    60481 ccccggtggc gatataggct tctttggaca agcctgagcg gatcagaaaa gcgcttcgga
    60541 gcgctccctg gtggccggac aatcctccga aaaaaccact gagcaggcct ccggccacca
    60601 tcttattttt atcaaattct atctttttaa gggagggcag gctttccatc agggcaaaaa
    60661 agaccagcaa agcggcgatg atgagtttaa tgggggtacc ataaaataat ttacctccaa
    60721 gttgatattc gaacaacggg gaccaggcgc tgaggttcaa caggagtttg gcgccgagat
    60781 aggcccccag tatggccggt acgccaaaac gcagaacggt ggtgacgtcc accttccggt
    60841 ttaataaaat gaatttaaat atattgttca gcaaatggac caccccggtc agggcgatgg
    60901 cggcatcgat cgggaaaaac agggcgaaga cgggcatcag cagggttccc agcccaaatc
    60961 ctgaaaaaaa ggtaagcagg gaaaccgaaa aactggcgag gcaaaggatc agtatttcca
    61021 tttctgcagg attataaatg ggtggcgatc aaaggcgcga tgtcaatccg gttgctttca
    61081 tgcgcaatgg tgtttaccgt tgctatttga cctgcatagg attttttcaa agaggcataa
    61141 gcgccttctg aaaatacggc atgaactcca atacaaaccg gtttttcaaa gccgagaacc
    61201 tccagcaaac ctgccgcggt gcttaaggta gtgcccgtgg aaatgatgtc gtccaccagg
    61261 accggggtat ggccgcggta ggcattcaga tcaggcaacc cgatttccac ctgacggtca
    61321 ccctgcctta ttttttgcaa tacttcatac gaagcccctg catcagaagc cacttccgac
    61381 acccattgcc ggctttcgct gtcggggcca aggacaaggg gtttcgggat atggattttg
    61441 atccaggaag cgatagccgg tgcggagcgc accacccggc tgggcaccga ataaatatca
    61501 gacagttgct taaatctgtg caaatgagga tccaccgtga tcagccggtc cacaaagcca
    61561 gaaataaatt ccgcaaaata agaggtcgta accgcctcgc cggtttgaaa agccatatcc
    61621 tgccgcatat acccaagata aggagccacc aataccactt cccatgcgcc cagatcttta
    61681 caaaggcggc ccaggaagta gaggggaagg ctgcgggtat cagcctgaac caaactgcag
    61741 atcaaaaaga ccttcttatt ttcgacggat gaatgcagcc tgaccagggt ctccccgtcc
    61801 ggaaaggtcc ggagttcaat tcgcccggtt ttaatctctg tgtgggtgga caatttttcg
    61861 gcaagggcct catttccagg cagggctaaa attatttttt ccatctgccg ctttattgaa
    61921 tggtgatcag gtcttcaatg gatgccaggt attctttgct gtacgccatt tccccggtag
    61981 attcactatg tatgcggaac aacaggtcac ccgcatttac ttttttgccg atcggggcaa
    62041 aaaattcaat gccggccgcc ggggatttgg gcgctccagc taatttagcc accttggcca
    62101 ggttacggtt gttgatttcc gtgattaccc cggctttcgt ggagcttaca tcaaattgat
    62161 attgagccaa agggggttcc ctgaaagcgc cctgggccag gcaaatggtt ttaaactttt
    62221 cataggcctt cccattttcg ataaactgca gggcatccgc ctttcctttc cctttttcag
    62281 aataattgac catttccagc attagtccgg ccagggcggc ggacttatgt tttaaatcgg
    62341 ccggagcttc ggtttcattt ttcagtaccg aaagcacatc ccgggcttcc agggccggtc
    62401 cgatgcccac gcctaccggc tggctgccgt cgctgatata ggtgtctacg ttgaggccga
    62461 tggattcacc cacttcgtgg aaatattgtt ttaatcgctt ggcatcgtca tggcttctca
    62521 ctttggccgt actgcctaca ggcatatcga tcaccacatg ggtggagccg gccgcggctt
    62581 ttttggaaag tacggaagcc accagctggc cttcactttc tatatccagg gctttttcca
    62641 cagcaatcag aatatcatct gcagggctga gttccatggc tccgccccag gcaaggcagc
    62701 ccccggtgtt gtagaccaca tttttaatgc tttcaatccc gaggtccacc cgggtgatga
    62761 cttccatggt gtcggccgta ccggccggtg aagtaatgga cctggaggag gttttcggga
    62821 tcgtgaaacc tgccgcggcg acaatactca ccacgatggg actggtccgg ttgccgggca
    62881 aaccgcccac gctgtgtttg tccaaaacga ccggtgaact ccaggaaagg cttgatccgg
    62941 ccgaaaccat cgcccgggtg agcgcggtga tctccggaag ggacagattg tccccggcgc
    63001 aggcggtgac gaaggcagcc agttcgatat tggaatattg tcccttggct acatcctgga
    63061 tgatttgcat caattgatcc tccaggaaaa atttgtcgta catttttccc cgtacataag
    63121 caaaggaacg aaccggtttt aaatggtgta tattgatgat atccccttcc cggacaccca
    63181 gttttttcat ggcttccgga gaaagacccg cttcatcgtg gcgcaccagg tttgacaaaa
    63241 cagccatcag ggtggctacg atggtttgac cattggcgct gacctgcagc cgggtcagcg
    63301 aggtgaaccc ttccgaattg cagatctcac aatcaatccg catataaatg gcatgctctt
    63361 tataggtatc gatgccgatc gattttacct taagttggtt gtaggacctg tggtcgtgca
    63421 tttccataga agtccaaaaa tagggatcag gaatcagtaa tcagtagaca gtttaaaagg
    63481 ggtcagcctt cgaaatagtc ggcaaacaaa tgtatttgtt tttgaatgag gtccaggtta
    63541 gcggacgctt cttcggttgt tttactttct ttgaaagcgc tcacctgttg aataaggggt
    63601 tccaaccaga gatggagagc atcgtggtca gggcctttca tccggcattc acggatcatt
    63661 ttgtccaggc ccgcctggag ttcggcggcc gttttgagat atgattcttg agtagggcct
    63721 tgcatgttta cggcccgttc cacgatggac tgaagatttt ttacgttttc cagggtgggt
    63781 acatccgatt tccatttggc cccctggttc agggataggc catgatccgg atgatcgtgg
    63841 acctgatggt tttccattgc tggttgttcg gggccggatg ctgaattttg tttggttttg
    63901 caggtgatcc agaggatgcc aagcagggag atcataagga tatttttcat ttcagccgga
    63961 tttggtttag aggcaaagat aaggggctat ggaataaaaa gcatttaaat acttatatta
    64021 tggaggcgaa tccggagcac gattaaacct ttgtccgccg gaaaaatctt tttcaacagg
    64081 gcttcacaat aaacctattt ttaggaaaag gaaattcacg atcaaccgcc aaccgtatga
    64141 aaaccatcat tgagcctttt aaaataaaat caatagaacc gatttatttt aatacccgtg
    64201 aagaaaggga aaaaatcctg gaggaggctt attacaatct ttttctcatt cactccaggg
    64261 acgtcctgat cgatctgctc acggacagcg gcaccagcgc catgagcagc gggcaatggg
    64321 cgggcataat gcaaggggat gaatcttatg ccggcagtcc aagcttttac cgttttgagg
    64381 aagcggtgcg tacagtcacc ggacttcccc tggtgattcc cacgcaccag ggccgggccg
    64441 cggagaaaat cctgtttcac ctcctgggcg gtaaaggcaa attcattctg agcaataacc
    64501 tttttgatac cacccgcgcc aatattgaat tttccggggc agaaggcata gacctggtgt
    64561 gcgaagaagg caaacatccc tcgttcccgg ctccgtttaa gggaaacgtg gacaccgcgg
    64621 ccctccaaaa atttattgaa aagacggggg cgcaaaacat tcccctggtc attcttactg
    64681 tgaccaacaa ttcgggcggc ggccagccgg tgagcatgcg aaacatccgg gaaaccaggg
    64741 ctatttgcga ccgtcacggc attccccttt ttatcgatgc ctgtcgtttt gcggagaacg
    64801 cctattttat caaactcagg gaagagggtt atgccgggaa accggttccg gaaattgtaa
    64861 aggagctttt ttcctatgcc gatggctgta ccatgagcgc caaaaaggac gcctttgcca
    64921 atatcggcgg tttcctggcc ctgcgcgatg aaaagcttgc ccgccaatgc agaaacctgc
    64981 tcattattac ggaagggttt ccaacgtatg gcggcctggc gggcagggac ctcgaagcca
    65041 ttgccattgg actccgggag gtgatggatg aacattacct tcagtaccgc atccgcagca
    65101 tcgagtacct ggccaacaaa ctcattgacg cgggcgtccc catcatccgt cccgccgggg
    65161 gccatgccgt gtacctggat gccaaagcgt ttaacacaca tattccgcca gaccaatacc
    65221 ccgggcaggc cctggcctgt gcgctttatt tggccggagg gataaggggc gtggagatcg
    65281 ggtcccttat gtttggcaaa tacgatgctg cccgtcgact ggtccctgca ccaatggagc
    65341 ttgtgcgcct ggcaattccg cgaagagtat acacccagag ccatatcgat tacgtggccg
    65401 aggtcgtcat cgaagtattc cggaaaaaaa gggaaatcca gggcctggaa atcacggaag
    65461 aggctcctat gctcaggcat ttcacggcga agctgagacc ggtgtccggg aatggaccgg
    65521 cagccctatt ttgaaggccg gcccgcgtgc actatttcct ttactatgta acaaaggata
    65581 cccttaaccc cttcccgaag aggcgaaatt tgtagtataa attatttatt atgaaaacgt
    65641 ctttaagata tggggtgctc ttcggagcat tagctctgat gcccctgctc aatagctgca
    65701 ctaaagagga atccattacc gaagactatg cgaattttga tgtagccgct atgcgtcagc
    65761 gggtaaacca atatcccgtc gcatccctga gcgccgaaga aatcaacagc ctcaatttca
    65821 tgcgggaaga agaaaaactc gcccgtgatg tctataacgg cctggcacgc cattggaaag
    65881 tcaacatttt caccaatatt gcggccagcg agcaaactca tatggacgcc atactgcaac
    65941 ttctgaataa atacggatta ccggatccga tagtatccga cggggagggg gtatttgcca
    66001 atgaggcgct gcgcaccctg tatctgcaat tgatggaaag gggatccgtg tccctgctgg
    66061 ctgcttatca gacgggcgct tatattgaag atctggatat caatgatttg aacaacgcca
    66121 gcgaattcat cgacaatgag gatatcctat atgtgtacgg caacctgaat aagggcagcc
    66181 gcaatcacct gagatccttt taccggaatg tcctccgact gaacggcacg tataccccct
    66241 cattcctaag ccaggaaacc tttgatgcca tcatcagcgg ccaaatggaa acgggaccct
    66301 ggtggtaaac cgaatgaaaa aggattaaca aattgaggtc atgaaaacct attggttgat
    66361 cgcgctcgtt ttgatgctgg ctcccagctt gcgggcccag gagacggcca ggctcagtgc
    66421 agaagatata gacgctatcc tgtttatgcg ggaagaagaa aaactggccc gggacgctta
    66481 tgaattgttt tatgtaaaat ggggagtgaa tcccttcggc catatccgtt tcagcgaaca
    66541 aaaccatatg gaccaggtaa aaacattgat cgtgtcttac aagcttgaag atcccattga
    66601 aaaaacggca gacgaaccgg gcaaatttga aaacgggcaa cttcagaact tatatgccga
    66661 agtggtaaaa gagggatcca cctcgatgat ggccggactg aaaacagggg ccctgattga
    66721 ggaaatcgat atcctggatc tggatgcccg aatggcccgg agtcaaaacg cggatatccg
    66781 tcgtgtattc agttatttaa aaagggcttc agaaaatcac ctgaacgctt ttgtcagaaa
    66841 gctcaaagtc aacggggtgg actataatcc ggtaaagatg aacaaaaaac aatttgatga
    66901 aatcctggga ggcaatgaac ggggatgctg cagagcaaag tgagttattg aatgatggcg
    66961 ttttaaggca gtacgtaaca attgatacca acctcctttg cgtattccga taactttgaa
    67021 atatcatatt gagataagtt tttgaataat agttcgagtt cagggagttg ggaatccggc
    67081 tccctttttt attggaggta tgagggcctt atctgaattc ggtttgtttg tataaaaagc
    67141 taacttgggc tatacttaca atcaaaacca gtccagatat gaaaacgaaa ccagatccga
    67201 aaggaatatc cagaagatcc gccttgcatt ctctcctcgc cctgaccgga tcggcggccc
    67261 tgccggtggc cgccaggggt catgaactca ctgaaggccc gagccgatta ttggcccggg
    67321 acaggacaaa gatcaaaatc accaaacttg aaaccttcct ggtcaaaccc cgttggattt
    67381 ttctcaaggt ccataccgat gccggagtag tgggcctggg tgaaccgctg ctggagggaa
    67441 gggcgctcac catccaaacg gccatcaaag aaattgagcc ttatctgatc ggcaaggacc
    67501 cccgggatgt ggttcaccac tggcaagcca tataccgcca tgctttttat aaaggaggcc
    67561 ctattctgac cagcgccctg agcggcattg atcaggctct ttgggatatc aagggaaaac
    67621 tgttgaacct tcctgtgtat gaattgctcg gcgggccgac ccgggaccgg gtccgcattt
    67681 atggaagggc cagtaacgcg gaacaaatga aagaaagggt caaagaaggc tacacggtga
    67741 ttaaaaccgg ggtagccaaa cagaatccct cgcgggcggt ggagaatccc aagtttataa
    67801 aacatgccgt ggagaatttt gcctccctcc gggaagccgg tggggatacc atggacatcg
    67861 gaattgattt ccacggggcc attcctccgc aaaccgccaa aattctgatt cgtgccctgg
    67921 aaccttatca acccatgttt atcgaagaac cctgtccgcc ggaaaatgta gatgtcctgg
    67981 ccgatatcgc caaaggcacc tttttaccaa ttgccacagg tgaacggata tttacaaaat
    68041 ggggtttccg ccagatcctg gaaaaaggag ccgccagcat tcttcaaccg gatctttgcc
    68101 atgcaggagg gattacggaa tgccggctta ttgccggaat ggccgaagcc tactatgccg
    68161 tcattgcccc gcacaacccc atggggccga tttccctggc ctcaggcatt cagcttgccg
    68221 ccagtattcc caatttcctg gtccaggaac aagtctcatt gggagagggt tatttaaaac
    68281 agcccttcaa actcgaaaaa gacggaacgg tattgatacc cacggccccg ggcctgggaa
    68341 ttgaactgga tgagaacaag ctggcggata agatcggtca cgactggaaa aaccctgaaa
    68401 cctatgatgc aacggatggt tcggtggtgg actggtgatt gtgcagatcc actttggtat
    68461 ctcagtgctg cgcgtgaatc atttgtggat ttattcgacc cctccagggt cgggactcag
    68521 gcggtttttc ccggggcgaa gccccgggtt accgacattc aatcccgggg ggattgtgat
    68581 gatcggccgt ttccgactat gcgtcacaga acccgctgaa ttttacgaaa ttttttgaac
    68641 actcactcac agacaacctg gatctgtccc gtaagggata agctgtgggt agatgattta
    68701 tgaattggat atttcgtccc gtcagggatg aaattccgga tgggacatct tttctttttc
    68761 gacctattta tttagtgcga tttcagcaca aaacttaagt ttcctgtttc ttaatcaaat
    68821 ccaacactcc attgataaaa gtcagcggat gaataggatt gccaggcaca taaagatcaa
    68881 tattatattt cgaaataaaa ctcctgtcca aggcagggct gccctcaaaa atgcccccgc
    68941 tgatggcatc ggtaccggca agaatgatga tcttgggttc cgggatggcg tcataacaga
    69001 tctgcagggg agaagccatg tttgcagtga tgggaccggt aatgaccagg ccatccgcat
    69061 gtcgcggtga agccacaaat tctatcccga aacggcccat gtcaaaattt acatttccgc
    69121 aggcattgag ctccaactca cagctgttgt ctccaccggc ggagacttgt ctcaatttta
    69181 aggaacttcc aaaataccca tgaatttcct ttctcaccag gacgggatcc agtttgattg
    69241 gatgttcttg ccctgattta acgatgagac ggtttcgatc attggtggcc agcatgtagt
    69301 ccgtcgtaaa acttatttta tccggaaatg caaatgcaca ttctccgcaa aaagtgcatt
    69361 tacccaggtc gatgcttagg ggattgaggg atatggcatt cgtcgggcaa agctccgcca
    69421 attctttttc cttcacgtcc acttcgctga ttaccggccg gcctctgaat atacccggga
    69481 cgattgcccg ggtgacatcg ggtatgtact gcctgccctg gtgaatcaat atttttaaat
    69541 tatccaacat aatcttcaaa ctgttttata aatcatggcc gcaataagac agatcaaaac
    69601 ttttatttgc aatggggaaa tccgaaattt cattatttcg tacggccagg gccaggccca
    69661 gccagttgtg aaatgaggga tctttgattt tataatgaat caattccccc ttcccgtctg
    69721 taatcgcaca atggcagatc tctcccctcc atccttccac caaggacaca caaaaggaat
    69781 ccggggctgg ctttaaaggg ttttgatcat aaacttcggg cgccgctaat atttttatta
    69841 aatgccggat atggtttatg gattggttga tttctttttt ccgcaaacgg gccctcgcat
    69901 agacatcccc atgcgttttc agtaccggtt gatgatccaa ggcctcataa gcatcgtgcg
    69961 gatgtgaggc cctgatatcc cggctgaccc cgctcatccg ggccgaaagg cccaccgtgc
    70021 caatggcgag ggcctggccg gtagtcaaca ctccggtctt ttcaaaccgg gccagggcgc
    70081 tgggcaggct gaatagttct ttggagatgt cctcaaaatc attttcatat tcgtccaaaa
    70141 agtccatcag gccaatggcc agatcccggg taaacggaaa atggctttta ccgggccgga
    70201 tcaaaccctt ggccaggcgg ttaccacacc atttctgaaa aaagttgatc accggtgttc
    70261 tcagcctgcc aaaaaccgca ctgcccaatt ggtacgccac atccgtgcac atggcgctta
    70321 aatccccggt atggatggca atcctttcca gttccagggc tatggtgcgg gccattttta
    70381 aatgatcccc ggtttgatgt ttacaaaggc tttcccaaag atgggcaaag gcaaggctgt
    70441 gtcccacgac ggaatccccg gcgatgtttt ctgccaaggt acatcgctga agcaacttgt
    70501 ttttttccag gaataaggac tcgactcccc ggtgctgata acccagctgg atttccagat
    70561 gtacgatttg ttcgccattg catataaacc ggaaatggcc cggctcaata attccggcat
    70621 ggatcggccc cactccgacc tcatgtaatt cctcactatc cactttaaaa aacggataat
    70681 cggctatccg ggaacccgga tcaaaccgat tgaatggaaa ccggacgggc ttcagccagg
    70741 gatggtccgt ataggctatt ccgaaatttt catggatctc ccgttcaaat ttttcgaata
    70801 cgagattatg tttggagaag gagggcaatg ccatccgggg caccgcagaa gaagatacct
    70861 gaatcgaatg ggattcatca ttcgcgatac aacaaaccag ccggatcagg ccatccaagc
    70921 ggaacccgaa atagttgaca caatgggctc ctttttcacg gatcagatta aatacattca
    70981 gttccagaaa ggagtcgtaa tccagcatgg gaatcccgga cagcgggatg gtttgatggt
    71041 tgcggacgct gataaaattc atatttaacg gggcagatgg tgaatggcct cttcgatgag
    71101 ccggacaaaa acgtccggcg ggtttaagcc caggtagacc gccagggcaa gcagcacaaa
    71161 ctgagaaagg gattccaccg gttttatgat ttccaggttt ttatcgttaa accccgtcgc
    71221 cggagaaaat acgagcttaa agatattttt ggcaaaggcc catatgatga cggtcaataa
    71281 gaggacgacg ccggccagga tcaataaatg attggcctca aacaaagagc ggaaaatcat
    71341 gaattcgctg atgaacaatc ccgaaggcgg catggcggag gcggaaaaaa aagccagcag
    71401 cagcacgatc gctccgtgca ggttgtattt aaaatagtcg ccgacatcgt acacgttttt
    71461 gctttgatag acccggtata cctgtcccat ttggaaaaaa aagctggact tgaccagggc
    71521 atggcatacg atatgcagga cggcgccata atagcccacg ccgcccgccg caatgcccag
    71581 catgacaatc cccatatgct ccaccccgga ataggcaaac agccttttca ggttcttcac
    71641 tttgaggata taaacggatg cgacaaacac ggaaaggaaa gccgtgatca ggatgatcgt
    71701 attgacccag gaatgcaacg gagtttgggc taccaccccg taaaaccgga agatgcccac
    71761 aaaacctaca ttgaccaggg cactcgaaaa cagagcgccc gccggtgaag gagccttgtc
    71821 ctttgcatca atacccgccg taaacatggg caccaggccc agcttggccg taaaaccggt
    71881 gaaaataaat ataaaagcca ggcgcagcca gaacaggttg agccggtcag cgttttttaa
    71941 cagggcggca aaggaaagat cgtttgcaac ggcctggggg agggagaaac ttaagaataa
    72001 gatcccgatg aataccaggg taatgctgat ggcgcacaca aaaacatatt tccaggtccc
    72061 ctccagggaa cggttgttcc tgcggtgata gatcagggct gaagcgctga gggtggtgat
    72121 ttccacaaaa atccaggcta ctgcaatatg attggccagg taggcggcgg agatcgaagt
    72181 gatcagaaga accatggcgc caaaataaat ggcacgatgc ctgatatgat cccggttact
    72241 gatcaaaaac acccggctgt ggtaaaaggc tgggatactg atcaggctca ggaccatcag
    72301 taaaagaaca gccagcgcgt caggggtaaa aaaaaccagt tccggcgtat tgagatgggc
    72361 gtattcatag atggtaaatc cggtctgaag taaaagaaaa cccaggatca aaaggaacat
    72421 atacctcctg ttcctggtaa aaaacagtaa accgcctatg gtgaatccgc cggccaaata
    72481 caatccagtc attcctttaa tctttcagtt ggctcaattg atcgacatcc acatcccgca
    72541 gcacatcgcc gatccggttg acaaatattc ccagcaggaa tatgctggca aaaatgtcga
    72601 gcaggatgcc cagattaacc agtaccggca tttccgtacc tacggccagg gacaggataa
    72661 aaaccccgtt ttcaatcacc aggtatccca taacatgagt gatgagtttg tttctgctgg
    72721 caataatgaa caagccggta aaaagcgtgg acagggcgac cacaaaataa attttactga
    72781 tccgcgcatc gggaatcgtg ttggaaagca gaaaggtgga aataataata agggtgatga
    72841 taatgagtga cataaaatta ggcaggtaag gttctgcgtc ccgggtaatc cggtttctct
    72901 tcatgacata ctgtaaaaac aggggaatgg caatcgtctt aaaaatgacg gtctccaata
    72961 aaacaaaaga caggttggct agatctattt caatcagttc aataaacgcg atgccaaaaa
    73021 gcaggatccc ctggaaggct aagattttaa tatacgtcag caggcggtgg gcaataccca
    73081 ggtaaagcag ggtaatggta aagatgatca gcaggacatt ggtcatgaat ggtttatttt
    73141 atgggtcatt tttcaattga attgtcctaa aatcagtaaa acacccagca ggatcagcag
    73201 cgagaccgaa gacagggtaa ggatatattg cgcattgtga ttcatgcgga accgggccat
    73261 gaaggattcg atgatcccaa cggtgacggc aaaagcgatt tgaagtccga gaaaaatggg
    73321 aatggtaaga accagcggaa agtctcccat aaaaaggttg gcgatcagcc cgccatacat
    73381 ggccaatttc aacggggtgc cgtataagat catacccagg tcaaatccgc tgttgtccag
    73441 caccattact tcatggacca tggtcaactc cagatgggtt ttagggtcgt ctataggcat
    73501 ccggctgttt tcaatcagcg cgatcatgac caggacataa gtggcaagca ccatcaggac
    73561 ataagaaacg gaggacccga aatgcaggga atgaaaaatc tgttgaaagg aagtatagcc
    73621 ggtgagcagg gcaaaggatc ccatcagcag gaaaaaggcg ggctccgcaa acatggaaaa
    73681 cagggcttcc cggctggcgc ccattccctc aaaactgctg ccggtatcca tggccgaaag
    73741 gatggaaaaa aactttcctg cgcccagcac ataggcgaag aaaacaaaat ctccttcaaa
    73801 ggacaggatg cccttgtatt gtcccagcgg aatgaccagg actgccatga gtatggaagc
    73861 caggtaaacg ctgggtgcaa gctggaaaat aaaactggtc gtctgactgt agacggaacc
    73921 ctttttaaac aggcgggcga ggtctttcag gggctggaaa atgccgggcc ctttcctgcc
    73981 ggaggttata cttttggtcc
//
LOCUS       DAOQXT010000009        73664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_352_length_73664_cov_7.328003, whole genome shotgun sequence.
ACCESSION   DAOQXT010000009 DAOQXT010000000
VERSION     DAOQXT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 73664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 73664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73664
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_352_length_73664_cov_7.328003"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1169)
                     /locus_tag="PKM27_02385"
     CDS             complement(150..1169)
                     /locus_tag="PKM27_02385"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNR06133.1"
                     /translation="MKVRTFNFFTMHATRLQRFITIKGLLFDLTGYFKALGLTIPGLV
                     LVWVSGCKPGVQHSEADLTTLGVLQRQCLGCHHPYRELPGQLAPPMNQIRKHYLDRLG
                     ARDPFIVGMSSFMINPASGTSLMPEEVKKYGLMTKIDLPEGDLHQVAAYIYKHDMEMP
                     AWQETWTTFKNQSGFRDLSVSYATLVSNIAGEAKAVLGKNLLSAIQQKDAAHAVAFCN
                     TRAFPLTDSVAALYQARIKRVSDKPRNPMNRANEKELAYILELKEKLAKGEKISPKIT
                     ERNGIVTGYQMIETVPMCLQCHGIPGKNILPETQAKLRSLYPYDEATGYAENQVRGIW
                     VIEVR"
     gene            1306..3399
                     /gene="pta"
                     /locus_tag="PKM27_02390"
     CDS             1306..3399
                     /gene="pta"
                     /locus_tag="PKM27_02390"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNR06134.1"
                     /translation="MSKIIYIATTEAYSGKSLVVLGIVNMLLGKAFKVGYFKPIIDED
                     PSGKRDVDIETMLGHFNLDLPYEDTFAFSRHQAMTHLEEETRGDLIDTIIRKVKILEE
                     SHDFTVIEGSDYLGEGAAFEFETNITIAKNLNAPAIFVISGAGKTTDQILGTVQTALR
                     NFNARDVEVLAVIINKVDREKSDEIKKVLEAHNTNRAIIAVIPYLKDLQNPTMKEIFE
                     QLGGRLITGEAQLGNQVDNFITGAMQLPSFLNYITENVLIVTPGDRADIIIGTLQANL
                     SVNYPRAAGIVLTADMMPDRSVLKLIEGLPNSIPILLVPGNTFATTTRLGSIHSRLTA
                     DNTKKLELAIATFEKYVDMATLEERIITFVPEGITPHMFQYQLVKKARMAIKHIVLPE
                     GKDERILKAAARLINQEIVRLTLLGDPSDLAASIKQLGLNLDLQKVSVIDPIHDPRFN
                     DYVETLYELRKHKNVTLAMARDLMSDVSYFGTMMVYKGHADGMVSGAMHTTQHTIRPA
                     LQFIKTKPGISTVSSVFFMCLPDRVSVFGDCAVNPDPTAEQLAEIAISSAESSLQFGI
                     EPRIAMLSYSSGTSGEGADVEKVRQATALVRQKRPDLKIEGPIQYDAAVDPVVGAQKL
                     PGSQVAGQASVLIFPDLNTGNNTYKAVQRETDALAIGPMLQGLNKPINDLSRGCTVDD
                     VFNTVLITAIQAQEN"
     gene            3408..4616
                     /locus_tag="PKM27_02395"
     CDS             3408..4616
                     /locus_tag="PKM27_02395"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNR06135.1"
                     /translation="MHVFVINCGSSSLKYQLFRMPLEKPLCVGLVDRIGLDGGKWVHR
                     VSPGAEEKSIEINQSMADHGTALLEVVRLLTDPNAGLIKSTAEIDAVGHRVVHGGETF
                     ASTTRITDEVKSKIRDLFSLAPLHNPANLSGIEVAEKLFPGAIQVAVFDTAFHQSMPE
                     VAFRYAIPRTFYTREKIRAYGFHGTSHKYVTEQAGRLLNKTDAKLISMHLGNGCSMTA
                     VRGGRSLDTSMGFGPVGGLIMGTRSGDMDTAVIFHLIDQLKFSPSEVNELLNKKSGML
                     GLTGYSDMRDIRQAMEKGDREAQLAFHMYAYRIKKYIGAYTAVLNGLDALIFTGGVGE
                     HDEMMRQTICSELEYLGVCLDKGKNQSGPAGQRDISREDAPVRIFVIAANEELEIARQ
                     CYALIKPGEK"
     gene            4613..5776
                     /locus_tag="PKM27_02400"
     CDS             4613..5776
                     /locus_tag="PKM27_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNR06136.1"
                     /translation="MTMRGLGSIRLKGVSLLMPVMLWVNASPAQNPKRTADYAIRIGV
                     MPAGKWNAITDVAGVKVGHTTLILGDSVRTGVTAVLPHEGNVFKQKVPAAVYTGNGFG
                     KLAGVTQVKELGNLETPIVLTNTLAVGTAMNAVVGYSLHLVGNELVQSVNALVGETND
                     GYLNDIRGRHVKEADVLDAIRTASKGPVMEGNVGAGTGTVCFGFKGGIGTASRVLPEK
                     LGGYTVGVLVQSNFGGVLVIDGVPVGEELKKYYLSDVLNDPADGSCMIILATDAPLDA
                     RNLERLAKRAFMGLARTGGIASNGSGDYVIAFSTDSSSRIPFQAPALIQTKTLSNDAL
                     SPLFMAAIEAAEEAIINSLLAAETMKGYKGRMVEALPGDKLKEILKKYGRVSK"
     gene            5856..6818
                     /locus_tag="PKM27_02405"
     CDS             5856..6818
                     /locus_tag="PKM27_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR06137.1"
                     /translation="MKKVAILVPETAVPAAIVDPRYLFTAVNEFFRSAGHQPFFDVQL
                     VGMSRKVALNGGLVVIHPELLLEKSGQMDLILVPALSGNMAEAVKRNRAFVPWILSQY
                     KRGAEVASLCLGAFLLASTGLLNGKTCSTHWLFANDFRNMFPEVGLAADKVITEQHGL
                     YSSGGASSYWNLLLYLVEKYTNREMAVMATKFFQLDIGRQSQSPFMMFRGQKEHEDVE
                     ILKAQEFIEKNYRCKINVDELAYRFKIGRRTFERRFKKATSNTPIEYIQRIKIEAAKK
                     QLESGRKTVNEVMYEVGYNDHKAFRDVFKKIAGMSPQEYRNKFN"
     gene            complement(6811..7293)
                     /locus_tag="PKM27_02410"
     CDS             complement(6811..7293)
                     /locus_tag="PKM27_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06138.1"
                     /translation="MFFSFFAWVFAFQALFHPLKKHMPVYAMAGFFIAVFSCIGGNNF
                     GIDGIYGTQMGVDSLEEKTALHEAIGPSTLAYLFIPGALFPLSLLIMGINLVRKKVLP
                     DWVGIMICIGAVGFPISRIPRIDLLAHADNLILWSSHLLAARYLSIRGYLKSGNSMLS
                     "
     gene            complement(7440..7991)
                     /locus_tag="PKM27_02415"
     CDS             complement(7440..7991)
                     /locus_tag="PKM27_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR06139.1"
                     /translation="MKISYPHIIENCIGEQIIFREVVQEPDGDRVLLENFVQPGHGPA
                     MHTHFKQEEMLEVISGKLGYQLLGEEPKYAGPGESVLFKRGIAHRFWNAGEVVLNCKG
                     WIKPAHSIVFFLTALYAAQNKSGKSEPESFDGAWLMTRYASEYDLPGIPVFIKKCIMP
                     LTCLIGKLLGKYKKFSDAPPPLR"
     gene            8050..8685
                     /locus_tag="PKM27_02420"
     CDS             8050..8685
                     /locus_tag="PKM27_02420"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR06140.1"
                     /translation="MNTEEKILAKALEMFNERGIEYVGLRELAGILGIRVSNITYYFA
                     TKDDLVNRISLELSAANEAVLRAEREPGLHTFLDTMRIIFNNHMRYRCIALSLVHILD
                     QNEVISERFKKTQLARRNFLTTSLSILADGGWVAFPDPADRDALASMIALLARFWIPD
                     SRFTVHKLQPPEQIRYYLHLLVALFNPFLTSKGKKETRKFIDEISGPSDKK"
     gene            complement(8714..9553)
                     /locus_tag="PKM27_02425"
     CDS             complement(8714..9553)
                     /locus_tag="PKM27_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HNR06141.1"
                     /translation="MDENPDVPIPVAHLFHELDLKLIELLHSLSPAEWTRQTLARQWT
                     VKDVTAHLLDTNIRVLSGLRDSHRTESPVIRNYEELIQYLNQLNADWVKAMKRLSPKV
                     LILLHEVTGPLTCDVYQSLKPYDTAPFAVSWAGETESKNWFHLAREYTEKWHHQQQIR
                     EATGRPGIMERKFFYPFIDTYMQALPYTYRDVYAPEGTVIKVHIPGEAGGSWYLTRSG
                     KAWVLGKSEINRPVAEVSISADLCWSLFSKNIRAGEIQQGIQLNGNLALGKQVLDMVS
                     VMA"
     gene            complement(9619..10152)
                     /locus_tag="PKM27_02430"
     CDS             complement(9619..10152)
                     /locus_tag="PKM27_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNR06142.1"
                     /translation="MKNLFVLICFNLVLSGLSAQVMLHPAIKNFGAMYDVPFGKDKPD
                     PSIDYKIIVDLGEKMEAPGQLYAPLEHISRMYNLHIYGGIPKNQLHVAVAIWGPSIAV
                     VMDNETYKKKYGVDNLNLQILREMKEAGIHIYGCGQSVAKFGLDPVHVNPDVEISISR
                     FTTVSTHQMHGYAVFHL"
     gene            complement(10236..10964)
                     /locus_tag="PKM27_02435"
     CDS             complement(10236..10964)
                     /locus_tag="PKM27_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNR06143.1"
                     /translation="MKTFILIHGSWHSSWNWHKVIPLLEAEGHRAIALDLPGMGRDKT
                     PIEQVRMKSTVDLICRRIDEIEGKVILVGHSKNGIMISQAAEYRPDKIEKLVYLAAYL
                     IPNGKTQSEYSAMDQAGMLKPYVTRHGHLNAHTLQPSIYREGLYADCPEEITELAKLL
                     LSYEPVESGLVPLELSETNYGRVPRYYIECTEDKAVTPFIQRKMYTETPCKKVYSMAT
                     SHSPFFSKPAELTEILNDIAQLQN"
     gene            complement(10986..11843)
                     /locus_tag="PKM27_02440"
     CDS             complement(10986..11843)
                     /locus_tag="PKM27_02440"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNR06144.1"
                     /translation="MADTENLNYQRISEAIEYLRLHFKEQPNLDEVAGQIHLSPFHFQ
                     RLFKEWAGVSPKKFLQYISVEHAKSMLKAHKVSLSDAAFETGLSGTGRLHDLFVQIEG
                     MTPGEYKNGGENLTINYSFAESPFGNLLVASTTKGICFMAFADDGKQAWNQLQHHFPR
                     AALTQKTDLLQQNALFIFSHDWNKLHEIRLHLKGTDFQLKVWEALLKIPMGKLATYGQ
                     VAEQVKSPGASRAVGSAIGSNPVAFLIPCHRVIQSTGLIGQYHWGPTRKTAMIGWEAA
                     RTKQAPSFS"
     gene            12064..13182
                     /locus_tag="PKM27_02445"
     CDS             12064..13182
                     /locus_tag="PKM27_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06145.1"
                     /translation="MNSRRHFLHKLSASLIALPAFSGRELNEIQLLNEKAYEGPVLRV
                     AILGLGSYGTRVADAMKTCTRAKLVGAVSGTPSKIKDWQTKYGIPEKNCYNYENFDQI
                     KNNPDIDAVYIITPNSLHAPFAIRTAKAGKHVICEKPMALNAKEGMTMIAACKAANVK
                     LLVGYRVHFEPKTLEIIRMRNEGELGKVMFFQGLCGFRIGDPSQWRLNKALAGGGSMM
                     DIGIYAINGSRYLVGEEPVWVTAQETKTDPVKFKEGVDETITFQLGFPSGVIASCLST
                     YSMNNLDRFYLNCEKGWAEMQPSTGYGPIKARTNKGELTHTHVTHQTVQMDEMSDIIL
                     GGKQPLVPVDGEEGLKDLKIIDAIFMAVKTGKKESLSL"
     gene            complement(13200..14126)
                     /locus_tag="PKM27_02450"
     CDS             complement(13200..14126)
                     /locus_tag="PKM27_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06146.1"
                     /translation="MIQTVLGKVSGEDTMTWLTHEHILVDFTGADSLDPHWNHDTVLQ
                     ATLPFLQELRDHRVAYFVDATPAYLGRDVRLLEKAAQKTGMQILTNTGFYGAVQNKYI
                     PAFAFALEPEALADLWTGEWTHGIEGTTVKPGFIKIGVDATDPLHPMQEKLVKAAAIC
                     HLKTGLTIASHTGEARGLWPQLKILEESRVSAGAFIWVHAQNEKENQNYLRAAASGCW
                     ISFDGLGWELPNHVDKLVYAREQGFLDQVLISHDAGWYDPGKTDQNIRAYTAIFRDLI
                     PALKAKGFRDEDLDLLLEKNPARAFSIAKRKL"
     gene            complement(14321..14686)
                     /locus_tag="PKM27_02455"
     CDS             complement(14321..14686)
                     /locus_tag="PKM27_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR06147.1"
                     /translation="MPLSPVNLAHKLSQFSDYFNPRIIAELNGQQVKLVKFEGEFIWH
                     KHDHEDELFMVLNGEFEMHFRDGIVTVRENEFLVVPRGIEHKPFARKEVAILLFEPSS
                     TLNTGDRNHDFTRKQLESI"
     gene            complement(14716..15219)
                     /locus_tag="PKM27_02460"
     CDS             complement(14716..15219)
                     /locus_tag="PKM27_02460"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR06148.1"
                     /translation="MKTRSLTFGFSLLFSLFLLSWDLPAQSEYKATHEGWLTNIDEAY
                     ALSQKTGKPILANFTGSDWCGWCKRLSAAVFLTEDFKKWAAKKVVLLELDFPRRKTLP
                     EKIRNQNYSLQQAFQVTGYPTVWVFDLEQEKATKKYNIKALGKTGYMASAKDFIAGVD
                     QMIAKRT"
     gene            15406..16017
                     /locus_tag="PKM27_02465"
     CDS             15406..16017
                     /locus_tag="PKM27_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HNR06149.1"
                     /translation="MKKWFFILILLNLHYLFAQAPIGGRCEGCEAVFENPVPFNQLDH
                     EVYLPDWNDPGPRMVIRGKVFKKDGKTPAEGVVLYVYHTDQKGIYPVRDQDKGWARRH
                     GYLRGWLKTNALGEYAIYTLNPAAYPNERFAAHIHVFVKEPDKNPYYIDEFLFEGDPF
                     LTENEINPSRPRGGKGLISLSKQNGLLAGKRDITLGLNIPGYH"
     gene            16026..16856
                     /locus_tag="PKM27_02470"
     CDS             16026..16856
                     /locus_tag="PKM27_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06150.1"
                     /translation="MQARIILCILAGALTQRSPAHPGPGIVKDSKGNIYYTDLTAVWK
                     INPSGIKTKVVTGIHTHLLHIDSQDNLYGEHLWYNGEHADTWGSYAWRLSADGRLDTV
                     LGPVTGFLEGLFSFDRDRWENEYWVQRGPVKKFFRKKPNGKIEKIGEARMENPGWIKV
                     SPDGRVYFVAGNHLYQLDTTGAFSILSRNIGSIILTRPPTSDPYVAGLWIHRDRIFVA
                     DYTGRQVKEVGMNGTVRVVARSEPPWSPVGGIFDNQGYLWLLENNENNTVNVRKIRTN
                     "
     gene            complement(16877..17326)
                     /locus_tag="PKM27_02475"
     CDS             complement(16877..17326)
                     /locus_tag="PKM27_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06151.1"
                     /translation="MANSDEINVLSICVDLLDGLDGSAAHRISTFLNNKYAESLKPSD
                     SKPILKKRGRPAKAALPAIGAGKKKRGRPAKAITLVAGTEKKRRGRPAKAVEPAATPV
                     KKRRGRPAKAVMPATALVAKKRGRPAKAITLVVGTEKKRRGRPKKSV"
     gene            complement(17462..17710)
                     /locus_tag="PKM27_02480"
     CDS             complement(17462..17710)
                     /locus_tag="PKM27_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06152.1"
                     /translation="MINSSVISAIRHINLGLICLRRYAFLPQQADAKVTLCGKGHFTQ
                     DPRWPAADGLASLMPPGLTMVERNHCDFNEKEMGFYWY"
     gene            17876..18355
                     /locus_tag="PKM27_02485"
     CDS             17876..18355
                     /locus_tag="PKM27_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNR06153.1"
                     /translation="MKTSIFNFLLALFFISGLYLVSCQSKGVKSDTPPLDVQELTKNQ
                     PETHGAEIKESDIVLATPLDQAMATKGNATYDVKCKSCHRLSEERLVGPGWKGVTKLR
                     KPVWIMNMITNVDMMLETDPEAQKLLEQCLVRMPNQNVTLTEAREILEFMRKNDGEL"
     gene            18407..20383
                     /gene="nosZ"
                     /locus_tag="PKM27_02490"
     CDS             18407..20383
                     /gene="nosZ"
                     /locus_tag="PKM27_02490"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNR06154.1"
                     /translation="MTSNKFFTKSALLMLILITWSACKPRGIQQAISSDASGKVYVAP
                     GTHDEFYNFVSGGFSGQMSVYGIPSGRLFRVIPVFSVDPEKGYGYSEESKPMLNTSHG
                     MVPWDDLHHIALSVTDGIHDGRWVFGNANNTPRIARVDLTTFRTAEIIEIPNSAGNHS
                     SPFITENSEYVVAGTRFSVPMDNGSTDVPISTYKDNFKGTVSFVKVDPSSGRMSIAFQ
                     ILLPGVNFDLSRAGKGKSHGWFFFSCYNSEQANSLLEVNASQRDKDFILCVNWKKAEE
                     YVNQGKGTKKKVRYAHNFYDEKKHMGTSTMETEVLVLDPKELADMVYMMPCPKSPHGC
                     DTDPSGEFIVGSGKLAATMPVFSFTKIQQAIADKTYDGDYEGIPVLKYEAVLHGEVQK
                     PGLGPLHTEFDANGNAYTSFFVSSEVVKWNVQSLQVLDRVPTYYSVGHLSVPGGPTKK
                     PWGKYMIAYNKITKDRFLPTGPELSQSAQLYDISGDKMKLILDFPTIGEPHYAEAIPA
                     ELVAKNSVKFYKIEENENPWAAKGDDFARVERKGNEVHVYMTAIRSHFVPDNIEGVKL
                     GDVVYFHVTNMEQDWDVPHGFAVKGANNAELLIMPGETQTLMWKPDRIGVFPMYCTDF
                     CSALHQEMSGYIRISAPGSNVPIKFSTGDLGPEQ"
     gene            20419..20970
                     /locus_tag="PKM27_02495"
     CDS             20419..20970
                     /locus_tag="PKM27_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNR06155.1"
                     /translation="MKTMVFIMIVLGMLSCKNTTTPDSTVGSSAEAPAAGQANVVDDE
                     SMKDVVKIAVGSPDHTTLVAALQQADLVNSLANAGPFTVFAPTNAAFDKLPAGTVDGL
                     MKPDKKTDLQDILQYHVTTSALKTSFFKDGMTLGMVNGGNISFQVKDGKIMINNSATI
                     VASVPASNGIVHVIDAVLLPPAK"
     gene            21010..22038
                     /locus_tag="PKM27_02500"
     CDS             21010..22038
                     /locus_tag="PKM27_02500"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNR06156.1"
                     /translation="MKNNNLTVFSRIVMAFATGMLVAVFFLPAWRIDLFAPQYPEGLK
                     MNIWLDRLTGDVDIINGLNHYIGMKPFSNESFPEFGWLKYVVYFFIAMGLLISLTGKK
                     RLLILYLILTAIGGTLAMYDFYKWGYEFGHDLDPKAAIQVPGMSYQPPVFGHKRLLNF
                     DSYSFPDIGGWLVISSAILAFLILVYEWYLHRKPKTTSPVAVLLITGALSATLSACQV
                     KPEPLVSGQDMCFHCKMTIVEPRYGCEIVTQKGKVYKFDDFSCMKSFIRSNTVKQEEI
                     RFTLVADFNNEKDWLPAETAIYYTGPLMKSPMGSNTGAFPDQAEASRFKGENEGTLSD
                     WNGLMQSQ"
     gene            22038..23267
                     /gene="nosD"
                     /locus_tag="PKM27_02505"
     CDS             22038..23267
                     /gene="nosD"
                     /locus_tag="PKM27_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNR06157.1"
                     /translation="MSRLIFFFWLLPAGLAAKVTDLYPHEDSTSIHNHFKEARDGDTL
                     RFNEGVYYIHSLVVTKPLVILGRGNCILDGSGKYEILTISARAGTVQGLTLRNSGYSS
                     MNDFAALKVIDAANLLITDNSILDSYFAIHLSNASRCTIRNNTIEGKAISEQLNGNAI
                     HAWKSHHLIIENNTVSRHRDGIYLEFVTHSRITGNHSHLQMRYGLHFMFSNDDVYTRN
                     IFEENGAGVAVMYSKRVTMKENLFRNNWGPSAYGLLLKDISDSEITQNRFEENTRAIH
                     MEGTSRIQLRQNLFKDNGWGVAIQASCNENSFSNNNFINNSFDVSTNGQVILNVFEHN
                     YWDEYEGYDLNKDGTGDIPYHPLSLFGLLIEQNPSMLILVKSFMMQLFERMEKAIPSL
                     TPEHFVDLQPLMKPFTP"
     gene            23264..23992
                     /locus_tag="PKM27_02510"
     CDS             23264..23992
                     /locus_tag="PKM27_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR06158.1"
                     /translation="MIRIEHISKEYRRIQALDDINLFFSRGQAVSLIGPNGSGKTTLI
                     KCILGLVLPDHGRIFVHTQPIETSPAYRNQIGYMPQIGRYPDQMKVGQLFKLLKDLRH
                     SDPAKLDYELYEEFNLSNLFESRMHTLSGGTRQKVSAALAFLFNPEILILDEPTAGLD
                     PLSSELLKKKIIVEKEKNKLVLITSHILSDLEDLCTHVVYLQEGKLIFYKDLPTLQQE
                     TREQKLSRAIAHFMKKNNPIAPLN"
     gene            23999..24766
                     /locus_tag="PKM27_02515"
     CDS             23999..24766
                     /locus_tag="PKM27_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR06159.1"
                     /translation="MLRLPRYIIYDILRNKIILAYTLLLFLISTGLFQLEENNDKALL
                     GLLSVVLIVVPLVSLIFTTIHYYNSYEFMELLLSQPQSRWSILLSEYSGVAVSLLVAY
                     GTGVGLPLLIYSPGPTGFYLLLAGLLLSLIFCSLAFLIAVLSRDKAKGIGIALLLWFY
                     FALIYDGVLLLLLFSLSDYPLEKLTFLFSALNPIDLGRIFVMLKMDIGALMGYTGAVY
                     KNFLGKETGILFTCLVNLVWVIVPLLLAKKIFNKKDM"
     gene            complement(24784..25572)
                     /locus_tag="PKM27_02520"
     CDS             complement(24784..25572)
                     /locus_tag="PKM27_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNR06160.1"
                     /translation="MSDHEIDYKIYGEEMQYVEIELDPGETAIAEAGAFMMMDEAIQM
                     ETIFGDGRHEDKGFFGKLMSAGKRVLTGESLFMTAYTNMGHGKNRVSFASPYPGKIIA
                     LDLYRLGGKMICQKDAFLCAAKGVEVGIEFQRKLGTGLFGGEGFIMQKLEGDGMAFVH
                     AGGHVLQRELQYGETIKIDTGCIVAFTPSVHYDIQFVGGIKNTIFGGEGLFFATLEGP
                     GSVWIQTLPISRLASRILAYGTVKRKDEGSILGGLGNLLDGDGR"
     gene            25659..26729
                     /locus_tag="PKM27_02525"
     CDS             25659..26729
                     /locus_tag="PKM27_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNR06161.1"
                     /translation="MKIRSIRFRLENLQLSKPYTIAWKTVDQVENLFCFLETDQGITG
                     YGSCNPENEVVHVSTKDTLEAMDLFDRDLLLNKSLDRPADLIESLLPSLHGVPTLLAA
                     LDIAVWDAWSKGLGKPLVEALGKKIGSLPTSVTIGIMEDDQTVKEAKEFLDRGFTHLK
                     IKIGLDPERDAARLVRLRKELGPGPFIRVDVNQGYRLEDFKKLYTACAELNIELFEQP
                     LRTDWFDQIDALPREVKSSIAADESLHGESDARQIASAGRCGIYNIKLMKCGGLHQAG
                     KLARIAETHDINLMWGCNDESRLSITAALHLAYACGRTRFLDLDGSFDLARDPASGGF
                     DLIDGKLIPLERPGLGIFMDND"
     gene            26738..27043
                     /locus_tag="PKM27_02530"
     CDS             26738..27043
                     /locus_tag="PKM27_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HNR06162.1"
                     /translation="MESFNTLIEAIQAYKEEGYTEDFNLQENCMVCSNGKYKLKAGDF
                     VIDKHYRFEDNTDPSDQAIIYAISSDKYDVKGILVNAYGIYSDDMASEMIEALSGRK"
     gene            complement(27090..28253)
                     /gene="uxuA"
                     /locus_tag="PKM27_02535"
     CDS             complement(27090..28253)
                     /gene="uxuA"
                     /locus_tag="PKM27_02535"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HNR06163.1"
                     /translation="MSMIQTMRWYGPHDAVSLWDLRQAGCTGVVTALHHIPVGEIWTP
                     EEIRKRKSLIEEAGMSWKVIESLPVSEDIKKGSGDYLRHIENYKTSIRNVAAEGLEVV
                     TYNFMPVLDWIRTDLEYPMPDQSKALRFEKPAFMAFDLFMLKRPGAEQDYREDQISRA
                     KRWYEEADEEKRSRVFHNCLQGLPGSTESFTAAEVLALLESYHHIDRDSLKKNLKYFL
                     RQIVPVAEERGVKMAIHPDDPPYPVLGLPRIVCTEEDAAELLDAVPSPSNGLCFCTGS
                     YGARADNDLVAMIRRFGDRIHFLHLRNTARDPEGNFFEADHLAGDTDMYAIMKEIVLL
                     QRRRKTNLPMRPDHGHQMLDDLNKKTYPGYSAIGRLRGLAELRGLELGVERSL"
     gene            complement(28269..28940)
                     /locus_tag="PKM27_02540"
     CDS             complement(28269..28940)
                     /locus_tag="PKM27_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNR06164.1"
                     /translation="MKTIETTAPPGTLNQNEILRMIYERRAVRKYKDLAVDRKDIEAI
                     VDAGRMAPSAINRQPWRFYVVDKKEDIAGLEKEVLKSAAKGILKSGFKTIIKSATRSL
                     HLAHGIDFLKNKNPIFHGAPVVVFLTAPEDNEWACLDLGMCAQNMMLAAKSMGLDSCP
                     IGLGKYVQGTKGYAKLKIPEKEKVMLALIFGYGDEKPSVHDRIRTNLFYPDLDAACGD
                     CETAE"
     assembly_gap    29291..29390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29391..30391)
                     /locus_tag="PKM27_02545"
     CDS             complement(<29391..30391)
                     /locus_tag="PKM27_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNR06165.1"
                     /translation="MPDSSSLYPGKSRQIVVIGMILGIMAFLLYSLNEFIVSFLGALI
                     FYVLFRKFMKRLVMVYRWKRWMAATLIIFISFLLIIIPLFGLVYLLIGQLQTTLSNPQ
                     DLMQGFNALLLKINRFFDRPVFSSDTLVEYIRGLADSVPAILNMSILALGSLLMMYFL
                     LYYLLVNTGHIEPALSRYLPFTREGIQMLTQELDDMTKANAIAVPMIALAQGIVSTFG
                     YWLFGMKDPLFWGVLTGCSGILPVVGAALIWMPAGIIMLVTAESWQGLGILLYGTLII
                     SLMDNFFRLIFARWFANVHPVITILGVIIGLQWFGLPGLVFGPLLIAYFFLLVKIYQ"
     gene            complement(30455..30967)
                     /locus_tag="PKM27_02550"
     CDS             complement(30455..30967)
                     /locus_tag="PKM27_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNR06166.1"
                     /translation="MNRTSSNRILILGIGNYLMGDEGVGVHAARRLEAQNRFPGVEVL
                     DGGTGGFHLLGCLEEYETIILVDATLDRHPPGTIRLIRPRFAQDFPKAMSTHDIGLKD
                     MMGALQLLGKMPDIHLFVVSIENIQLQGVSLSPEIERVMPQLLEKIEHLASSLTSPAA
                     QRMPQDLNPV"
     gene            complement(30974..31696)
                     /gene="cybH"
                     /locus_tag="PKM27_02555"
     CDS             complement(30974..31696)
                     /gene="cybH"
                     /locus_tag="PKM27_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HNR06167.1"
                     /translation="MEPKGLKIQMLRRVYVWELPVRFYHWLNALVMVALVATGFYIAD
                     PLALQSQREATGKFTMGWIRYIHFAAAYLFFFNFLARIYWGFVGNKFANWKQFIPVSG
                     TFFKEMWTVIKMDVLMMKNREHMSIGHNALAGFTYFLTFILFLIQCLTGFGLYAAMSD
                     WWFPQLFAWVPALFGGDILTRQIHHWAMWFFILFVVIHIYLVFYHDYIEGRGEISSMG
                     GGWKFIEEEAFEKEHTHTPNPE"
     gene            complement(31713..33440)
                     /locus_tag="PKM27_02560"
     CDS             complement(31713..33440)
                     /locus_tag="PKM27_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNR06168.1"
                     /translation="MSTRIVVDPITRIEGHLRIEADIENGKIKDAFSSGTMVRLLEEI
                     LKGRDPKDAWAFVGRVCGVCTSVHSLTSVRAVEDALDIVVPPNAEMVRNIMFCTQYMH
                     DHVVHFYHLHAMDWVDVVNALKADPKKTSELAQSISHWPKSSPGYFSDIQTRIKKFVE
                     SGQLGIFANGYWGHPAYKLPPEVNLIGLAHYLEALEWQKEIVKIHAIFGGKNPHPNYL
                     VGGMACAIGIDDVSGINAERLALVGQLLQDAKEFVEQVYVPDLMAIASFYKDWGAIGG
                     GLGNYLVYGDLPTNGFRDMASFKFPAGAILNKDLGNIQEVDLRKDEEVQEFVTNSWYE
                     YREGKTVGLHPWKGESKVNYNGPKPPFEHLDTAKEYSYLKTPRWNGHAMEVGPLARVL
                     VGYARGREDFKAVVDKALTDLKVPVTALFSTLGRTAARGLESVLIAQWGLEFFDQLIS
                     NIKNGDTRMAEMSKFDPATWPAQALGVGYSEAPRGALAHWINIEDQKIANYQLVVPTT
                     WNASPRDGKGQLSAYESALLNTPVADEKQPLEILRTVHSFDPCMACSVHLYDEEGKHI
                     SRISVVGGE"
     gene            complement(33464..34591)
                     /locus_tag="PKM27_02565"
     CDS             complement(33464..34591)
                     /locus_tag="PKM27_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HNR06169.1"
                     /translation="MIHETLPSPPTYYEEVERKGYSRREFMAFVTTMAAFMGLENSAI
                     GQVVHALETKSRVPVIWLHFQECTCCSESFIRSSHPIVADILLDKISLDYTETLMAAS
                     GVQAEEAMKHTMEKYRGEYILCVEGSVPTGADGVYCMIGGKTSLQILEEAAAGAKAVI
                     AWGSCASNGCVQAARPNPTNATPIHKLLKGIPLIKVPGCPPIGEVMAGVIVHLVTFGK
                     IPELDRLGRPKAFYSKRVHDSCYRRAYYDAGLFVESFDDENAKKGYCLYKVGCKGPST
                     YNACGVMRWNGGTSFPIQAGHGCIGCSEENFWDNGRFYERASAFAGFGIEANADTLGK
                     AALAATGVGIAAHAIATNVRKHKLIAGKVQEGKISEKEFES"
     gene            34747..34962
                     /locus_tag="PKM27_02570"
     CDS             34747..34962
                     /locus_tag="PKM27_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06170.1"
                     /translation="MKKFLLPVLTLLIGMLPLTGWSHPGHGESDGFTLIHYFREPLHA
                     VGPLGILVTLLIYRVRWSRNKARQQRA"
     gene            34965..35306
                     /locus_tag="PKM27_02575"
     CDS             34965..35306
                     /locus_tag="PKM27_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNR06171.1"
                     /translation="MHELSIVMCILDIAEEYAGKTKYRAIEEIELDIGCLTTIEMNAF
                     EFAWKEGVRNTRLQDAAKKINRIKGKAKCMDCENTFTIENLFDPCPSCGQHLLDILEG
                     KELRVKSLMMS"
     gene            35338..36099
                     /gene="hypB"
                     /locus_tag="PKM27_02580"
     CDS             35338..36099
                     /gene="hypB"
                     /locus_tag="PKM27_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNR06172.1"
                     /translation="MCATCGCGTVDSVHGHEGGLPHDHSHDHSTHTHKVVDVEKDILQ
                     QNNLLAERNRGYFEAKNILALNLVSSPGSGKTALLEKTLTDLKGQLEFAVIEGDQQTT
                     NDADRIHATGTKVIQINTGKGCHLDAHMVLHAVQGIKPKQNSVLFIENVGNLVCPALF
                     DLGERERVVIVSVTEGEDKPLKYPDMFHSSTLCIINKTDLLPYLPFNIEKLISNAKKV
                     NPKLEIIELSCTQGTGLNLWYDWLKSKVPVPEPVE"
     gene            36128..38473
                     /gene="hypF"
                     /locus_tag="PKM27_02585"
     CDS             36128..38473
                     /gene="hypF"
                     /locus_tag="PKM27_02585"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNR06173.1"
                     /translation="MQTYRIHISGLVQGVGFRPYVSRLAKEYNLKGWVSNTNTGVYIE
                     ITTEKEEALKFYHTLLEHPPENAVISRHHLVETAYATYARFTIRPSTGDDKPDLMPAP
                     DLALCADCRKELLDKTNKRFQYPFTTCLQCGPRYSITAGLPYDRANTTMQPLPMCKAC
                     EEEYRDTENRRHYSQTNSCKTCGIPMHLYSPPGVLLTSDPEKIMDSVQQALRSGLILA
                     VKGIGGYLLMCDATQESSIHTLRSRKHRPSKPFALLYPDCETAAGDVELRPFEIKALE
                     SKAAPIVLCTLKAGPESGICHAAIAPGLDKIGLMLPGTPLLFLISKAFGKPLIATSGN
                     LSGSPILFKDEDALQHLFEVADHIVTYDREILVPQDDSVMQFTAQGQQVILRRSRGLA
                     PNFFPNPFDPADGPVLAMGGELKSAFALLEDRNFYISQYLGDQGKLESQASYIQTLDH
                     LTHLLKAHPRHILIDKHPEYEVSRMGKQRAAEKNIPVTSIQHHKAHFGAVLAENDLLD
                     TREPVLGFIWDGAGYGDDGQIWGGEIFVYTDHHMTRMAHLDYFPQLLGDKMSREPRLS
                     ALSLLRGLPGKHHRLLPFFTLQEWTYYSSMVKKEPPLQTSSVGRLLDGIAALLGLCAH
                     NRFEGEAAMLLEAHARKCKDPSTGIYPLPLVHSRLDWRDLIAGVLEDLEQNRKTEDIA
                     WKVFYSLARGAAEVSRQCGIKRIAFSGGVFQNVLLNELLHRVFDEKNSLYFHRKTSPN
                     DECIGLGQLACYHILNRQPDKTGKLNRSTPVLTIRSNPLIL"
     gene            38475..38750
                     /locus_tag="PKM27_02590"
     CDS             38475..38750
                     /locus_tag="PKM27_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNR06174.1"
                     /translation="MCLAIPGKILSISRQLDETFQFGTVSFGGIHKEVNLCMVPEAKV
                     NDYVLVHVGVAISVIDEAEARLTFEYLKQMGEVNELKTGSDPGVSFN"
     gene            38770..39873
                     /gene="hypD"
                     /locus_tag="PKM27_02595"
     CDS             38770..39873
                     /gene="hypD"
                     /locus_tag="PKM27_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNR06175.1"
                     /translation="MKYLSEYRDPEMVDEFLQEIHRVTTKPWTLMEVCGGQTHSLVKN
                     GILDMLPPRITMVHGPGCPVCVTSVRVIDEAVFLAKQPNIIFTSFGDMLRVPGSDKNL
                     LEAKAEGADVRMLYSPLEALEIAKENPDKEVVFFAVGFETTAPANALSVIQASRLGIR
                     NYSILASHVLVPPAMEAILSDPENKIDGFLAAGHVCTIMGMEEYYPIAEKFKTPIVIT
                     GFEPVDLLQGILMTVRQLEDGKARVENQYSRYVRRAGNPMAIQTIQEVFAVSDKTWRG
                     IGSIPQSGYEVNEAYKWYNARLKFKLDLPAAPENPACISGAIMKGIKKPKDCPNFGTA
                     CTPEHPLGAPMVSSEGACAAYYHYSSPVEWAQP"
     gene            39945..40964
                     /gene="hypE"
                     /locus_tag="PKM27_02600"
     CDS             39945..40964
                     /gene="hypE"
                     /locus_tag="PKM27_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNR06176.1"
                     /translation="MPKFDYDKITLGHGSGGLLTHRLLKSGVFDLFKNSMMDQQHDGA
                     SFEIQGRQAFSTDSYVVSPIFFPGGNIGDLAVNGTVNDLAMCGASAKYLSLSFIIEEG
                     LTMESFWEILVSIKKAADKAGVMVVTGDTKVVEKGKGDQLFINTSGIGPIHPRAQIHH
                     HRIEAGDSIVVSGNIATHGITIMSLRKGLGFETDVRSDTTNLNHTVIHLIDKFGEDIK
                     FVRDPTRGGLASVLNEIAELTQLCYQLDQKSIPIDPQVEGACEMLGLDPLYVANEGVF
                     VAVIKKEITGEFLDLLKRDERGRQAALIGEVNTSYPGKVILKSRIGGSRVVNYLTGEQ
                     LPRIC"
     gene            41060..42316
                     /locus_tag="PKM27_02605"
     CDS             41060..42316
                     /locus_tag="PKM27_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR06177.1"
                     /translation="MYQIFRVNKVVSLIPALALVFLSYTISSGQKIKRAALREEMDSI
                     LLAHVDRGDIPGAVVQIKKGKKRIHRQAYGYARLYDSQKRVLPDPEKMTVNHIFDLAS
                     LTKVIGTTTALMWLVDHRQISLDDPVAKYLEAFNTPEKMGIRIRHLLSHSSGLYEWYP
                     LYYRASSRAESYSLIASLPLKYKLGEDRHYSDLGFVILGQLIERVSGMTLDQFMEQKI
                     FKPLGMRKTLYNPQQKKHFRKIAATSHGNPYEKRMVYDSTLGFQVKGIDPEAWNGWRT
                     HTLMGEVNDGNAWYANGGVSGAAGLFSTARDVQKVIDLILNPDRVRPFISKKTIDSFL
                     SKDQYKNGLGWMMDTTYSFIRGGPEGSFGHTGFTGTSIAVIPACNISIVLLINRQHTG
                     LSDQGLYYNVGPIREQVFKAVLKYCR"
     gene            complement(42475..44208)
                     /locus_tag="PKM27_02610"
     CDS             complement(42475..44208)
                     /locus_tag="PKM27_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR06178.1"
                     /translation="MTFKKKILVLLSVGCLFFNQGCDRILDEQERSSLSPEYFKTPEG
                     IQAGVVAAYSLMRFLWGTEGFSYSTSAGTDEMLRGDGSSSNLFTYGILTSEGTAQAIW
                     DISYQAINNCNGVIELGPAANMPAATKQAVIAEAKFLRAWYYFLLVQTFGEVSLNLNF
                     VTEPSTSASRQPIADVYAAIIKDLKEASVELPDKPSPSPGRAAKAVALHVLAKVYLTK
                     GWSSAATGDDFANAQATAQQLIADKAKYGLDLWQDFFDANKEGNEYGKETIWVIDRNT
                     DATASESGYGLGFGANNPKQNGSLFYWRPNYSGVALNVNAGISGLPVNNNVMMDRDVV
                     NGRPFLRFRPSAYTQNVAFGERVNDTRYNKTFQTVWIYNRTITINTLQGPLRRGIDTA
                     IWMIGREVTEEERRAFRGVLFTPSQYTGSMYPAMKKYDDITRLTPNDPSDRPFIMFRF
                     ADTYMVAAEAAFKAGKLDDAANHINVIRQRAAFLSSRNAEQNAAAANAMKITTADLNI
                     DFIMDERTREFYGEYQRWYDLVRTKTLKDRLAKYNPIAGFKDFHYLRPIPQSQINLVT
                     TGTPYPQNPGY"
     gene            complement(44235..47345)
                     /locus_tag="PKM27_02615"
     CDS             complement(44235..47345)
                     /locus_tag="PKM27_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06179.1"
                     /translation="MIKDPMLFMVRVRKLLIPLLMVLNAPVFAQSITVTGTVSDENGE
                     GVPGATILVKGTSTGTATDVNGYYSLNAAPDAILVVSYVGYQVNEVAVNGQASINIQI
                     QPSASSLEEIVVTGYGTQKRRDVTGSIVSIKEQTLREVPSANVIGALQGQIPGVDIAR
                     NGVRPGTAGSIRIRGNRSLGRTVGENEAQNSPLIIVDGIPYGGSINDLNPDDIAGLEV
                     LKDASATAIYGSRGSNGVILITTKRGKPGKAQISYNGYIGQVSQTDQYRLLTGPEFKK
                     FKEDARYNAGGGIVEFSVDELAGIANGTSTDWYDLVYQDGSVMNHELSVAGGSETTQY
                     GLSGGFYKETGNLPGISFNRASIRSTIDARIGNRIKVGLNTLNTLSNNHGEGINPMFN
                     IMRISPLLSPYLPDGSLNLFPLLGTVDGTATANPLTLNNKDAIKDSRRRIRTFNSLYG
                     EVDLVKGLKYRANIGLDYRQDYRGQYRGTNTIINPSGQTQANIDNGEAWSYVIENLLT
                     YEKTFAERHRLTLTGLFSVQEDQGFSSAFQATSLPADYVQEYNFTLANSVVVNNNGNG
                     YSKRGLVSYMGRLNYVLDDKYLLTATLRRDGSSVLSPGNQWFTYPAVALGWNIANEAF
                     LKNSKFITGLKLRLGWGITSNQSVQPYGTLGGLSRNNYNFGATNVLGYFVSALPNKNL
                     KWESTTNQNLGLDFGLFRDRIMGSIDVYTQKTKDILVSKSLPISNGAGSFLTNAAQTK
                     GHGVEVSLSSINVQTPGGFRWSTDLNFSINREEIVALEEPGKLRDIGNGWFVGQPLTV
                     IYDFKKLGVWQTSEAEEAKKFNSIPGRIKLEDANKDGKIDANDMQVLGSFQPDWIGGL
                     TNRISYKNFELSAVMFARMGGQVVATYFQSNAGGAGGYAFFGQGRVNQMKVDYWTPDN
                     PTNEFPQPDGLTNNADYYSTLGYHDGSFIKLRSVNLGYNFPEKLLTKYGIGSLKVYVT
                     AQNPLILYSPFVRDGYGIDPEGTGYGGALAPQGSGTAGAPGRAITVGINTPPTRMFNA
                     GINLKF"
     gene            47479..48504
                     /locus_tag="PKM27_02620"
     CDS             47479..48504
                     /locus_tag="PKM27_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR06180.1"
                     /translation="MEREKEVTIYDLAERLNLSIATISRALKNDPVVNPKTKKRILEL
                     AQKMGYRTNYFAKNLRTRRTNTIGVIIPRLNSYFMSTVIAGMESVANKAGYNLIISQS
                     AESFEKEINSARTMFNNRVDGLLVSLAYESNNLDHFKAFLEKKIPVIFFDRTEDQQSF
                     TNIVLDNRKAGYDATAHLISQGCRRIVHITANTMRNVYIDRMRGYQEALRDAHIPFDE
                     QLVLKGNLSMEFGHAAADQVLALPTRPDGIFAANDNCAVSCMLRLKQAGIQIPRDIAV
                     VGFNNDPVSVVVEPNLTTIDYPGYEMGELAASHLISHLSGSQLLQKTKTILLHHELLV
                     RKSSQRS"
     gene            48551..49297
                     /locus_tag="PKM27_02625"
     CDS             48551..49297
                     /locus_tag="PKM27_02625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR06181.1"
                     /translation="MEQEKKVALITGGGSGIGFGIAEKFIEKGMQTLLVARDEEKLKA
                     ACAQLGASCHYKTCDLADLKTIPALIQNILSEYGRIDVLVNNAGINMKKDFTEVTDED
                     FQKIITVNLCSVFSISREVVKDMIRLKSGSIINISSMAAQYGLPRVIAYSASKSAIEG
                     MTRAMASELSPHGIRINCIAPGFIETAMTAKALNDDPPRKAKALGRTPMGKMGKPADI
                     GAAAAFFASDESAYITGTVLCVDGGNAIGF"
     gene            complement(49452..50924)
                     /locus_tag="PKM27_02630"
     CDS             complement(49452..50924)
                     /locus_tag="PKM27_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06182.1"
                     /translation="MNKKINRERRDFVKKSGIAAASFFIVPRYVLGGRGYTAPSDRLN
                     LAAIGAGGKGQSDIANAWNNGANQVVALCDVDTARCKASIDKFSSAKLFKDFRRMFDE
                     MKNDIDAVTISTPDHTHAIAAMAAMQNGKHVYVQKPLTHNLAEARMLTEAARKYKVVS
                     QMGNQGASNPGQQQMIEWFSKGLIGPVDQVYVWTNRPVWPQGIPVPKDKPALPASMSR
                     EDWDLFIGPAEYVDYHPLFHPFKWRGWWNFGTGALGDMGCHLIDPPFRVLGLGYPTEV
                     ECSVGQVFTQDWQPEYIPEGCPPSSHVQLKFPATAKNKKPITLTWYDGGIRPFHPEWI
                     PASEDLGDAGSTNGVMMIGTKGVMTCGTYGLIPKIYFKNGKKIEMPKDYMTSNPNEKL
                     PEYGHQVSWTQACKAGFGSKEHQALTSSFDYSGPLTETVIMGNLAIRSYSLGTPGQNN
                     RLSFNGRRKLLWDGKNMKVTNYDEANQFVSRPYRQGWKMA"
     gene            51128..53707
                     /locus_tag="PKM27_02635"
     CDS             51128..53707
                     /locus_tag="PKM27_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNR06183.1"
                     /translation="MNKLWQKILRITNPSGKAIPPGRYKLARLLWGLMMGGVLFIILF
                     FIFLSFQNLPTFDELENPQYNYATVVYFDDETELGRLFKQNRVGVNYDDLSPYLVQAL
                     LSTEDNRFYSHAGVDFEATARVIGRTMLLADKSSGGGSTITQQLAKLLYSNRDFRGMS
                     NMEKKLALVLIKFKEWITAIKLERSYTKEEIIAMYLNQVEFVYDAFGIKAAAETYFDT
                     SQDSLKIEQAATLVGMLVNPARYNPKRFPQNSLYRRNLVLDRMHTSGYLSRAQRDSLK
                     QIPLETSAFRRRDVSEGNAPYFILELSKSLQDMFRNSDLKKPDGSNYDIYKDGLKIYT
                     TLDPVMQELAEQAVWAHMPKIQKQLFTVWKNSDPWVFEADDRQKEIRRYKLNSLVRES
                     DRFLGIRDKILGVLADSIDRRFDFVLTDNDILRMLEEENKAGKINDYIHQGILSNERA
                     SKYRQLMDDPLWPELVKAWKLVLAEADKEFNTRIRMKVFDYNESHKKDTSMTPMDSLR
                     YMQMFLQTGTMAIEPGTGKIRTWVGGIDHRYFKFDHITADRQVGSTFKPFIYATAIGF
                     KAISPCLKVVDRPYTISPGEGNFRLISPWSPKNSEGRWTETPLSLYQGLAYSVNSVSV
                     FLMKELGNTEIVCGLVNAMGIDSSKRRPDGEYRVPRQPSICLGSSDLTVMEMTGAYSA
                     FANDGKFIKPYFIDRIEDKNGRVIFQAKIEESRALPSDVNYVMVDLLRRASPVRDGSV
                     KSIHGGKTGTTNSYKDGWYMGVTPGLVVGTWVGGDLPWIRFKTLAQGQGSVMAKPIFA
                     EFLKRLEADPRANWDITRDFYKPDRIGIEMNCAAYDSLHIQDQNLIKQSKKLEDEFDD
                     FEY"
     gene            53716..54933
                     /locus_tag="PKM27_02640"
     CDS             53716..54933
                     /locus_tag="PKM27_02640"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR06184.1"
                     /translation="MFLLIDLPVIRIRPFLFVFWFIPVFPFIGCTGPEVPVEHVHSRP
                     PTALDSFMIDFSDYFSHQVSICNCPGAALAIVEDTTISLVRGYGLRSVDAADPVDEHT
                     LFRLASVSKGFGAVLTAKLVDKGLLHWNDPVNQYVPDFKVNPEEETPKIAISSILSHS
                     LGLPYHSYTNLIEAGKSITEIIPLFRDVRLNLLPTQKYAYQNASFALIEKVAEKASGG
                     KPFTSLLQQELFSPLRLKDMSFTYEDMINSPNKALPHRADHTGRYHPVKITPKYYNAV
                     SAGGINASASDMAVWLHLLLGHYPEVLSQNALDSVFAPHILTSTDRRHYNFWPGVKET
                     YYGLGWRILDLGDHYRYYHGGYANEYRAEIAIDPKYKIGICGMFNAPCDVADYLIPAF
                     FQHYEQWKQLRNN"
     gene            55015..56052
                     /locus_tag="PKM27_02645"
     CDS             55015..56052
                     /locus_tag="PKM27_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06185.1"
                     /translation="MGKNRIKRQPRIPSLKGRQLSLVQTKKEWEGKSLLAKKDQKTYG
                     KIIESNLLAFNQDFLRAKGDSDKIHKAKKEARTRLMKELKRSIPGVENLLKSKLNPPA
                     EFVRFRELQKNILGNTNIDTSLETAPAGLLDYRVFEPPFALGISGEATPWFLDDYTDG
                     HLTRNSSTLEPSGFMSLNFDAKHDSTPVIPFVSWGFYSMLLSAGTDYTIPQTGYLNIT
                     AVARNLYNQVLISILDKAGLSVSEMNISHEIGLLIVRSGEFYFYPQVIFKDGLINHGG
                     LDQVHLLPGFPTHIPHFLNLTTSDAFLKGETVQIRFYSRLSYSYKTVCMTNYVLARLN
                     WKLDKLYLKMT"
     gene            complement(56234..58684)
                     /locus_tag="PKM27_02650"
     CDS             complement(56234..58684)
                     /locus_tag="PKM27_02650"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06186.1"
                     /translation="MKTTVLILFSMLSLARPLVAQNTGGITGRVVDKNSQQALEAVNI
                     QVEGTSRGTSTDSAGYFQITNIPVGTYSVLFSSLGYKTYTLYNIVITSGNESNYTIEL
                     EEEISTLSEIVVRGTTRTVKAATIETPLSVQKVTTEEIRSNPGGNFDISKVIQTLPGV
                     GGGAGGGSFRNDIIIRGGAPNENVFYLDGIEVPVINHFQTQGSSGGPQGILNVSFIEE
                     VKLASSAFDAKYDNAVSSVFQFKQKNGNPNQFQGNTRLSATEFAATLEGPVSREKNST
                     FLVSARRSYLQFLFQAIDLPIRPNFWDFQAKLNLPLDAKNTLTFIGLGALDEFRFAAP
                     KNSTPEKLYVINSNANINQWNYTAGVSLKRLLNNGYLNLALSRTAFDNDIVKFEDNQN
                     PEPSTQTLDLNSRETENKLRLDINKNLGQWKLAYGASVQYVEYSNKTSALLRKEIRDP
                     DNNILQPGVTANFDSPLDPFARIGAFVQAGKRFASNRLGVSAGLRTDMNTFTTGAMNP
                     FNTLSPRISLSYSLADAWTLNASAGKYFKLAPYTILGFADNQNRLVNRDADYLGSTHY
                     ATGLEFIPGEDLRFTLEGFYKKYSRVPVSVRSGISLSNLGRDYSVLGNEAVITNGKGR
                     AYGLEFFAQKKLTERFFGILSYTWYHSQYSGADGIFYPSSWDNRHLLSLTWGYKFKRN
                     WELGLKFRYQGGAPYTPFDETASRLNYLSQGTGVLDYSMLNSQRLNSFHSSDIRIDKK
                     WNFRRTTLDLFIDVTNWYVARNPEIPEYTFRRNEDNTAFLTSDGQALKPDGSNAIPTR
                     VKNDDPFFTPTIGFIVEF"
     gene            complement(58880..59242)
                     /locus_tag="PKM27_02655"
     CDS             complement(58880..59242)
                     /locus_tag="PKM27_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HNR06187.1"
                     /translation="MKLITHHIDNIKIAELTSEEILINKTEDGLDLLGNLYFQGFDRI
                     LIKERDINPAFFDLKNGMAGELLQKFSNYRVRLAVVGDFSKYKSKRLKEFIFESNKLG
                     HIHFVNSQSDAIKALSSN"
     gene            complement(59283..>59700)
                     /gene="arr"
                     /locus_tag="PKM27_02660"
     CDS             complement(59283..>59700)
                     /gene="arr"
                     /locus_tag="PKM27_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HNR06188.1"
                     /translation="TKNASPFAQTYFHGTRSDLKTGEFIEVGFNSNYGQRKNAKYIFL
                     TATLDAAIWGAELASGEGRERIYLVEPTGPIEDDPDLTDKKFPGNPTQSYRSTHPFKI
                     VGEVVHWQNHAPEQVKAMKEGLEKLKAQGIDSLNDQ"
     assembly_gap    59701..59800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<59801..60395)
                     /locus_tag="PKM27_02665"
     CDS             complement(<59801..60395)
                     /locus_tag="PKM27_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06189.1"
                     /translation="MIKRYSVLICVILSLILMLAATWVYPGGSLLDKNSTGFDWTKNF
                     ISNLFSVKAMNGVDNPSRFWAIPGMAFHALGYGLFFINMASKISANHAARVLKMIGYT
                     NIFLNILITTRLHDLMIIITSTSFLMGLFYITVYLLRTRLHFIKVCCLLGLLTFYYTL
                     YLYGAGDWALLAIMQKVSLFGSMGLVLGLEYFTEKNDF"
     gene            complement(60448..60975)
                     /locus_tag="PKM27_02670"
     CDS             complement(60448..60975)
                     /locus_tag="PKM27_02670"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNR06190.1"
                     /translation="MSILSFFYYERVSFNKYLENQIVQYNMNIPINEKAPVRSQNQIE
                     IEASLDVVWETLTDIPKWPEWKKDIKKAVVHGEIKQGTEFDWKAGGLSFQSRIHTSNP
                     KSGFGWTGTTFGASAIHNWFFELKGNTTVVRVEESLQGVFPKLFKGYFQKNLDSGIIK
                     DLQELKGAAEKKVQR"
     gene            complement(61160..61858)
                     /locus_tag="PKM27_02675"
     CDS             complement(61160..61858)
                     /locus_tag="PKM27_02675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNR06191.1"
                     /translation="MGILAFVITPGVVSVTNWPRLIVYWVAVLLGLVWQFILSLLILK
                     QEGHALNWQTVAKRTQYRKPVNPKTGKSSYWLFLWAIPFILLSAVLQSGISGLPNVDG
                     LMAPLIQDLPKYDLSSLAAAEYRGAWWILGLYLITAIFNYLLGEEFFYRGILLPKMKG
                     VFGNWDWFFNGVLFGFYHLHKPQIILSTALYFGFEFALPSKLFQSSWMAVIIHGLEGV
                     LGIVVILGIILGYT"
     gene            complement(62012..62656)
                     /locus_tag="PKM27_02680"
     CDS             complement(62012..62656)
                     /locus_tag="PKM27_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNR06192.1"
                     /translation="MQTILSKKWLSLTIYCGLSAFLSYFLAAFAPFHEKISVVLAFAF
                     GPLFMLASVGLYFILRQWRDSINLRIAVLFNIVATAMLTMMLTVQLTIQAYHQQFKAA
                     DRGSVSEEQLKWIYQEVNSVQLGMDLAWDIFISIGTFFFALVMYKHPKIHKILSVLGM
                     VFAILLLGFNLAYFPEPPGEAGSVDFGPFVAIWYMALMVWMLINKKTVNAEEYT"
     gene            complement(62828..63472)
                     /locus_tag="PKM27_02685"
     CDS             complement(62828..63472)
                     /locus_tag="PKM27_02685"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNR06193.1"
                     /translation="MRLLLVFIGFAALVLLAFALWGDSLMLAFSQEGTLQWLDRFGPW
                     AWLGAIMLLMADLVLPLPATIILSALGFLYGPWLGMLIGTAGSFLAGSLGYWLCRMMG
                     QKTALVLLGRKDFERGQKISSKLGSWIVVLSRWLPVFPEVVSGMAGLTRMNAPLFHLA
                     LLTGTLPMAAVYAWVGASGYEHPYWAVGLSMGLPPLIWWGVGGIINRELKNERN"
     gene            complement(64130..64579)
                     /locus_tag="PKM27_02690"
     CDS             complement(64130..64579)
                     /locus_tag="PKM27_02690"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNR06194.1"
                     /translation="MPKPKPNPVQPSEIPALARDLVKKARFPTLATLDGDQPRVRPVS
                     PVRTEGFVVYIANLRSYGKTAEIASNPKVELCYMDEDHNQVRITGRAEMVRDRALLEE
                     IWSANRLLQHYLGSIDNPELIVYKILPERVRYMVEWALEYYEVPING"
     gene            64657..65247
                     /locus_tag="PKM27_02695"
     CDS             64657..65247
                     /locus_tag="PKM27_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNR06195.1"
                     /translation="MEKDYKQVLLRILNTYAPVDKTAADLMFASGSMEKVKKNEIVFQ
                     ENRFEAFEYFQLEGVSHRYNLDEDMQALTTGIYQGETVITPHFARTTNGQSIFTLQAL
                     MDSVYLKIPAGLFREISDQNQQIRMFGRAVIEKEFIRSLNFEILFRAFNAKDRLIWFR
                     TKYSSLENLIPHTIIASFLGITPVSFSRLRNDLARK"
     gene            65353..66522
                     /locus_tag="PKM27_02700"
     CDS             65353..66522
                     /locus_tag="PKM27_02700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR06196.1"
                     /translation="MVSFLRKMKRILILISLLTLGLLIVIRPANKSYRMKNIDLFLKD
                     QVDQGRTPSIQYAFFDTGRVIFESHYGWRDLISRKAVDASTTYHLFSITKTFTALAVL
                     QLAQSGKIGLDKPVSTYLPVFPYPEDITIEQLLSHTAGIPNPLPLKWIHLAEEHPSFR
                     RDPFFAEVFKAHPKTDFEPGTKFKYSNLGYVLLGQLVEAVSGQPFEEYVNQNILARCG
                     PGLSFELDPSSHAVGYHKWWTFTNAIFGFLIDRSKYMGRKEGKWVPFKPFYNNGTAYG
                     GLFGSASGLIRYAQNLLDPHSVLLNNDYKEILFSEKVVRNKTTGMSLSWFTGKLKGHE
                     YLAHAGGGGGYYCELRIYPRLGVGSVILYNRSGMRDERILDRADAFFITEKPRQE"
     gene            66736..67302
                     /locus_tag="PKM27_02705"
     CDS             66736..67302
                     /locus_tag="PKM27_02705"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR06197.1"
                     /translation="MKQKLKTTLAFTKNLFTTGAFMETSEKVEKEICKHLSPEENTLV
                     VEYGMGHGNITQNILKHISKTSRVYAFEVNPDFCDFVRKAIPDDRLTIIQDSAENVKN
                     HVKQEVDSVISSIPFTFLSKHQSEKILADSYELMRKGSYFSQVLYTKFNFKKFVPVFD
                     QCEIIRLKHFPTEYIYHCLKNGKRKELA"
     gene            67439..69388
                     /locus_tag="PKM27_02710"
     CDS             67439..69388
                     /locus_tag="PKM27_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNR06198.1"
                     /translation="MDLRPGVILLCLLCFAGSLTAEDSVRYETDVRPLLQKRCISCHN
                     TNAPKGGVNIDNYKEQDRVIKDGQFWLKVLDQIKTGAMPPKSEPKLSEADYHGLVDGI
                     NSILQTSLRQRSAGHVVIRRLSHSEYRYTILDLMQVDFDARNYFPSDGSGGGGFDNQG
                     RALFFTPLKLERYYDAADHIVEQAYKDPKIWGDLVPVEFRQNVFQRLGNWFLSLFTDS
                     YRQFNAPENAAEKVIYPFATRAYRRFLKDEEKTRLIDLFSSVYHRKDSVKNPQRFNES
                     IAQVYKAVLVSPNFLYRTEEEPEKMGSYPLTQFEVATRMSYFLWSALPDQELFELARA
                     GRLDDTLVLEAQVKRMLADPKSRRFAENFSSQWLGITKLIDPQPIADPELFPDFNMRI
                     RKAMYDESVEYFYHVLTQSKNFLDLIQSNYTFLNKDLAEYYGIPGITGDQLVKTTLPD
                     STRGGVLGMGSVLTTTSFATRTSPVNRGKWVMEQIMGISPPPPPEVVAELTEDKAIHD
                     KLGLRKILERHRADPNCNSCHVKMDPLGLGLENFDPIGRWRDSYGMVPVDASGVLSDG
                     RPFKGPGELKSLLLTEKAKFARNLSTKMLSFALGRSILFTDEPALQVLESTLLATDFN
                     PEPFIVELVKSYPFRMKLNDFEKKI"
     gene            69393..70772
                     /locus_tag="PKM27_02715"
     CDS             69393..70772
                     /locus_tag="PKM27_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNR06199.1"
                     /translation="MNKKWHITRRRMLKGLGASIALPYLEAMALPAWKPSGVVKAPIR
                     SAFLFMPNGVHPDRWTPSATGRDFALTQQLQPLANLKKDILVLGELMNKNSIFKGADG
                     HYAKSASLLTCMPIKQTVGDNISSGGVSVDQLIASKIGHETLFPSLEYGLDRITTGVD
                     INVGFTRLYGSSISWATPAQPMSKEIDPRLAFDRLFRTFVPGKKIKEDPWKQSILDAV
                     MEDATSLRKNLGRADQDKLTEYLESIRSVEKRISNGTNLEKFKANITPDIRKELVRVN
                     TRIDEYVEVYGGVDITEKTRLMLDIMALAFWSDASRVSTFMFGNSVSNRNFSFLEGVK
                     GNHHSISHHMDKASFLEEYTRITAWHVEQYAYLLEKLKSLKEGDSNVLENSMIVFASD
                     LRDGNRHSPRNLPILLGGQGGRKIKTGQNLNYAKETPLANLYLTMLQAMDIEEERFGD
                     STGTLAGIA"
     gene            70805..72283
                     /locus_tag="PKM27_02720"
     CDS             70805..72283
                     /locus_tag="PKM27_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06200.1"
                     /translation="MMHAKPGIPRPLLAVLLGLIGLGFLFLPGLAPPAEIPVIALLLG
                     RAHPLVLHFPIVLIMLALVFELFCRFKVVERSETFIRVILILSLLSFVAAIASGYFLF
                     ASGEYSGRLMTSHFWGAAISGAAAFLTAGIYFFLPKNSGLFSLYLSGLFVSNVAIVYT
                     GHLGGSLTHGRDFLSEYTPLLFQKPESSGPKDASEMLVYTDLIAPVLEAKCISCHNSQ
                     RAKGGLAMDSYASLFSPGESGQPSVTAGDPEKSESLKRIYLPADHPDHMPPEGKSPLS
                     QDEIRLLRYWVQTGASDSLRLTEASKTDSIALAIENMRPVLARYQHKAEAASLNQKII
                     RAELESLAEKLQVIIEPDSLGEANEYSLSMKFPPAPFTNDQIQSLKPYFPYFSKLSLV
                     SSGIDDDGLYYLGKMENLRELYLLKTGLDGSGLVHLQHLKKLEILNLSYTKTDDQSAF
                     DLMKIPNLKTVYLYRTNVSRQVMEAIMKYKRGLILLEEEGVF"
     gene            72368..72886
                     /locus_tag="PKM27_02725"
     CDS             72368..72886
                     /locus_tag="PKM27_02725"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HNR06201.1"
                     /translation="MSPSLFPRMHVSLYVSDLDQTIAFYNSFFGQSPVKVKTHYAKYV
                     LEQPSLIISFVEHKDRVQSHFGHLGFQVETPEVMHAALDRMKESGLETREEMGVNCCF
                     AKQDKFWVMDPDGVEWEVYYFHEDAEFNDPRYEMEDQKVQKTAMTEIFSTPVKEPCCA
                     PGEGSSGGKPCC"
     gene            72980..73552
                     /locus_tag="PKM27_02730"
     CDS             72980..73552
                     /locus_tag="PKM27_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNR06202.1"
                     /translation="MDIHFFKNQQEFRDWLDKNHGEAREIHVGFYKKETGKPSLTWPE
                     SVDQALCYGWIDGIRRSLGPESYTIRFTPRKPRSIWSAVNLAKMEILIREKKVRPAGM
                     AIYEKRDLDKIKKYSFEQEKDPTLSPALLRSFKSNKKAYAWFKASAPSYQKAAIHWVM
                     SARREATQNTRLNTLIEDSAQGRRIGLMRR"
BASE COUNT        18523 a        18991 c        18029 g        17921 t
ORIGIN      
        1 tcacataacc gaataattta atgtgttttt tatgagttgt gcaacatgca cagctatcag
       61 ctatcagcta tcaaataaac ttctgattac tgactattga ctattgacta ctgactactg
      121 actactgact attgactact gactactttt tacctcacct caatcaccca tatccccctc
      181 acctggtttt ccgcataacc cgttgcctca tcatagggat agagggacct gagctttgcc
      241 tgggtttccg gtaaaatatt tttgcccggg attccatggc actgcaggca catgggtacg
      301 gtctcgatca tctggtagcc ggtgactatt ccattgcgtt cggttatttt ggggctgatt
      361 ttttctcctt tcgccagttt ttccttgagc tcaaggatat aggcaagttc tttttcgttg
      421 gcgcgattca tcgggttcct gggtttgtcg ctgacccgtt ttattctggc ctggtacaat
      481 gcggccacac tgtcggtcag gggaaaggcc ctggtattgc aaaacgccac ggcatgggcg
      541 gcatcttttt gctggatggc ggacagcagg tttttaccca aaacggcttt cgcttctccg
      601 gcaatgtttg aaaccagggt ggcataactg acggataaat ccctgaagcc ggactggttt
      661 ttaaaagtcg tccaggtctc ctgccaggcg ggcatttcca tatcatgctt ataaatatag
      721 gcggcaacct gatgaaggtc cccttccggc aggtctatct tggtcatcaa tccgtatttc
      781 ttaacctctt ccggcatcag ggaggtcccg gaggcggggt ttatcataaa ggaggacatc
      841 ccgacgataa atgggtccct ggcacccagc cgatcaagat aatgtttacg gatttgattc
      901 atgggtggag caagttgccc gggcaattcc cggtaaggat ggtggcaacc cagacattgt
      961 ctttggagca cacccagggt agttagatcc gcttccgaat gttggacgcc gggtttgcag
     1021 ccggaaaccc agaccaggac caggcccggg atggttaatc caagtgcttt aaaatatccg
     1081 gtcagatcaa aaagcaggcc ttttattgtg atgaatcgtt gaagccgggt agcatgcatg
     1141 gtaaaaaaat taaacgttct gaccttcatt aagccagaaa tctaaaaata tccatttatc
     1201 gtcatattca tactgtccgc ggtcaccgcc ttcaaacctc caaagccgct tccggtccac
     1261 tgactgcgat taagctattt ttgtaccgca tttcgtaatt tttatatgtc aaagatcatt
     1321 tacatcgcca ccaccgaagc ttacagcggt aaatccctgg tcgtcctggg cattgtcaat
     1381 atgcttttgg gcaaagcctt taaagtgggt tatttcaagc ccatcatcga cgaggatccc
     1441 tcgggcaaac gggatgtgga tattgaaacc atgctcggcc atttcaacct ggatctgccc
     1501 tatgaagaca cctttgcatt cagccgccat caggcgatga cccatctcga agaagagacc
     1561 cgcggcgatc tgatcgatac catcatccgc aaggtgaaaa tcctcgaaga atcccatgat
     1621 tttaccgtga ttgagggcag cgactacctg ggggaaggcg ccgcctttga atttgaaacc
     1681 aatattacca ttgctaaaaa cctcaatgcc cccgccattt ttgtgatttc cggcgccggt
     1741 aaaaccaccg accagatcct gggtaccgtg cagaccgccc tgcgcaattt caatgcccgc
     1801 gatgtggaag tgcttgccgt gatcatcaac aaggtcgacc gggaaaaatc ggatgaaatc
     1861 aaaaaagtcc tggaagcaca caatacgaac cgtgcgatca tcgccgtgat tccctatctc
     1921 aaagacctgc aaaatccgac gatgaaggag atctttgaac agctgggggg aaggctgatc
     1981 acgggggaag cccaactcgg caaccaggtc gacaatttta tcacgggcgc catgcaattg
     2041 cccagtttcc tcaattatat cacagagaat gttcttattg taactcccgg agaccgggcc
     2101 gacatcatta ttggcaccct ccaggctaac ctgtcggtca attatcccag ggcggccggc
     2161 atcgtgctca ccgccgatat gatgcccgac cgatcggtat taaaactgat cgaaggcttg
     2221 cccaattcca ttcccatcct tctggtgccc ggaaatacct ttgccaccac tacccggttg
     2281 ggttccattc attccaggct taccgccgac aataccaaaa agctggaact ggctattgcc
     2341 acctttgaaa aatatgtgga catggccacc ctcgaagaac gaatcatcac ctttgtgccg
     2401 gaaggcatta ccccgcatat gtttcagtac cagttggtga aaaaggccag aatggccatc
     2461 aaacatattg tactgcctga aggtaaggat gaacgaatct taaaagcagc cgcccggctg
     2521 atcaaccagg aaattgttcg cctgaccctc ctgggcgatc cgtcggacct tgccgcatcc
     2581 attaaacaat tgggtttgaa tctcgacctg caaaaagtat ccgtcatcga tcccatccac
     2641 gacccgcgtt tcaatgacta tgtggaaacg ctttacgaat tgcgcaaaca caaaaacgtc
     2701 accctggcca tggcccgcga cctcatgtcg gatgtttcct attttgggac catgatggtt
     2761 tataaaggcc atgccgatgg catggtatcc ggggccatgc atacgaccca gcatacgatc
     2821 cgcccggctc tccagtttat taaaacaaag cccgggatat ccaccgtatc ctccgtcttt
     2881 tttatgtgcc tccccgaccg ggtctcggtg tttggggatt gtgccgtcaa cccggatccg
     2941 acggcggaac aattggcgga gattgcgatt tcgtccgctg aaagcagcct ccaattcggc
     3001 atcgaaccgc gcattgccat gttgtcttat tcttcgggta cttccgggga gggcgccgac
     3061 gtggaaaaag taaggcaagc taccgccctg gtcagacaaa aaaggcccga tctgaaaata
     3121 gaaggaccga tccagtatga cgccgccgta gatccggtgg taggagctca aaaactcccg
     3181 ggttcacagg ttgccggcca ggccagtgta ttgatctttc ccgacttaaa caccggaaac
     3241 aatacttata aagccgttca acgcgaaacc gacgccctgg ccataggccc catgctgcag
     3301 gggctcaaca aacccatcaa cgacctcagc cgggggtgta ccgtggatga tgtttttaac
     3361 accgttctca tcacggccat ccaggcccag gaaaattaat ccctgccatg catgtctttg
     3421 tgatcaattg cggcagcagt tccctgaaat accagctttt caggatgcct ttggaaaagc
     3481 ctttgtgcgt gggtctggtt gaccggattg gtttggacgg aggtaaatgg gttcaccggg
     3541 tttcccctgg cgctgaagag aaaagcattg aaataaacca atccatggcc gatcacggga
     3601 ccgctttgct ggaagtggtc cgcctcctga ccgatccaaa cgccggtttg attaaaagca
     3661 cggcggaaat tgatgcggtg ggacaccggg tggtccacgg tggagaaacg tttgcctcca
     3721 cgacccggat cacggatgag gtcaaatcaa aaatcaggga cttgttttcc ctggcccctc
     3781 tgcacaatcc ggctaattta tcgggtattg aagttgcgga aaagctattt ccgggagcga
     3841 tccaggtggc cgtttttgat accgcctttc accagtccat gcctgaagtg gctttccgat
     3901 atgccattcc ccggaccttt tatacccggg aaaaaatcag ggcctatggg ttccatggaa
     3961 cctcccataa atacgtgacg gagcaggccg gccgcttgct gaataaaacg gatgcaaaat
     4021 tgatttccat gcatctgggc aatggatgca gcatgaccgc ggtacgggga ggacggtccc
     4081 tggatacctc catgggtttc ggaccggttg gagggctcat catgggtacc cgttccgggg
     4141 atatggatac tgcggtgatc tttcacctga tcgatcagct aaaattcagt ccctcggaag
     4201 taaacgaact tctgaacaaa aaaagcggta tgctgggcct caccggatac agcgatatgc
     4261 gcgatatccg ccaggcaatg gaaaaaggcg accgggaagc acagctggct ttccacatgt
     4321 atgcctaccg gatcaaaaaa tacatcggtg cttataccgc cgtgctcaac ggactggacg
     4381 cccttatttt tacaggcgga gttggggaac atgatgaaat gatgaggcaa acgatttgct
     4441 cagaactaga atacctgggg gtttgcctgg acaaaggaaa aaatcaatcg ggtccggcag
     4501 gccaaaggga tatcagcagg gaggatgctc cggtgaggat ttttgtaatc gccgccaatg
     4561 aggaactgga gatcgcgcgt caatgttatg ccctgataaa accgggtgaa aaatgacgat
     4621 gcgaggcctt ggttccatta gactcaaggg agtgagcctc cttatgccgg tgatgctgtg
     4681 ggtgaacgcc tctcctgcgc agaatccgaa acggaccgca gattatgcga tccgtatcgg
     4741 ggtgatgcct gccgggaaat ggaatgccat caccgatgtg gccggggtga aagtcgggca
     4801 taccactttg atcttgggcg attcggtgcg gaccggtgtc accgccgtcc tcccgcatga
     4861 agggaatgta tttaaacaga aggtgccggc ggctgtatat acgggaaacg ggtttgggaa
     4921 gcttgccgga gtcacccagg tgaaagaatt gggaaatttg gaaaccccca tcgtgctgac
     4981 caataccctg gctgttggca cggccatgaa tgcagtggta ggttattcat tacatctggt
     5041 gggtaatgaa ctggtccaat cggtgaatgc cctggtggga gagaccaatg acggttatct
     5101 caatgatatt cgcggcaggc atgtaaagga agccgatgtg ctggacgcca tccggaccgc
     5161 gtccaaggga cctgtaatgg aaggaaatgt gggcgccggg acgggcaccg tttgttttgg
     5221 tttcaaaggg ggaatcggga ccgcttcacg ggttctgcct gaaaagctcg ggggctatac
     5281 ggtaggggtc ctggttcaaa gcaattttgg cggagtactg gtcatcgatg gggtcccggt
     5341 aggcgaggaa ttaaaaaaat attacctgag cgatgtctta aacgatccgg cggatggaag
     5401 ttgcatgatc atccttgcca ccgacgctcc attggatgcc cgcaacctgg aaaggctggc
     5461 caaaagggcc tttatggggc tggcccgtac cggaggaatt gccagcaacg gcagcgggga
     5521 ctatgtcatt gctttctcca cggacagcag ttcaaggatc ccgtttcagg ctccggcctt
     5581 gattcaaacc aaaacattga gcaacgacgc cctgtcccca ctcttcatgg ctgccattga
     5641 agccgccgaa gaggcgatca tcaattccct tttggcggca gaaacgatga aagggtataa
     5701 gggaaggatg gtggaggcct tgcccggtga caaattaaaa gagatcctga aaaagtatgg
     5761 ccgggtctcc aaatagaaag ccgatcccaa accccatagt tcaaaaatcc atggttgcgg
     5821 gtggattgcg acagaaaggc tgctatcttt aataaatgaa aaaagtggcc atcctggtcc
     5881 cggagactgc ggttcccgcg gccatcgtgg atccccgata cctgtttacc gctgtaaatg
     5941 aattcttccg aagcgcaggg caccaaccct tttttgatgt acaattggta ggcatgtcca
     6001 ggaaggtcgc gctgaatggg gggctggtcg tgatccaccc ggaattgttg ttggaaaaat
     6061 ccgggcagat ggacctgatc ctggtgccgg cattaagcgg gaatatggcc gaggcggtca
     6121 aacgaaaccg ggcttttgtc ccctggatcc tatcccagta caagcggggc gcggaggtgg
     6181 cgagcttatg ccttggagcc tttttacttg cctccaccgg tttgttgaat ggcaaaacct
     6241 gttcgacgca ttggcttttt gccaatgatt tcaggaatat gtttccagag gtgggcctcg
     6301 cggcagataa agtaattacc gaacaacatg ggctctattc cagcggaggc gcgagttcat
     6361 actggaacct gctcttgtac ctggtcgaaa aatatacgaa ccgggaaatg gcggtgatgg
     6421 ccaccaaatt ttttcagctg gatatcggca ggcagagcca atcgcctttt atgatgttcc
     6481 gggggcaaaa ggaacatgaa gatgttgaga tcctgaaagc ccaggaattt attgaaaaaa
     6541 attaccggtg caaaataaat gtcgatgagc tggcataccg attcaagatc ggccgccgga
     6601 cctttgaacg gcggtttaaa aaggcgactt caaacacgcc cattgaatac atccagcgga
     6661 taaaaatcga agccgccaaa aaacaacttg aatccggccg gaagacggtc aatgaggtga
     6721 tgtatgaggt gggttataac gaccacaaag cattcaggga cgtttttaaa aaaatagcgg
     6781 gtatgtcccc gcaggaatac cggaataagt tcaactgagc attgaattac cagatttcag
     6841 atagcctctt atggacaaat acctcgcggc gagcaggtga ctggaccata aaatcaaatt
     6901 gtcggcatgg gccagcaaat caatacgggg tatgcggctt attggaaagc ccactgctcc
     6961 aatgcaaatc ataataccca cccagtctgg caataccttt ttccgaacaa gattaatacc
     7021 catgatcaaa agggagagtg ggaataaggc gccgggaata aataaataag ccagtgtgga
     7081 aggacctatc gcctcatgaa gggcagtttt ttcttccagc gaatccacac ccatctgggt
     7141 accataaatg ccatcgattc cgaaattgtt tccccctatg cacgaaaata cggcgatgaa
     7201 aaacccggcc atggcgtaaa ccggcatatg cttcttgaga gggtgaaata aagcctggaa
     7261 agcaaaaacc caagcgaaaa aggaaaaaaa cataagcact cctgcagttt tagtgactac
     7321 cccctgatac caaaaaaact gggagaggac cagaaaaaac ggggccagga tcaagccggc
     7381 cagccatatc cggctacgaa tggactcata tgaatttgga gtattcatca ttacagggat
     7441 tatctcaggg ggggaggtgc atcgctgaac tttttgtact ttcccaaaag cttcccgatt
     7501 aaacaagtca ggggcatgat gcactttttt ataaaaaccg gaatgcctgg taggtcgtac
     7561 tccgaagcat aacgggtcat gagccaggct ccatcaaaag attcgggttc gctttttccg
     7621 gatttgttct gagccgcata cagagcggta agaaaaaaca cgatggaatg ggccggtttg
     7681 atccatcctt tgcaattcag gaccacctcg ccggcattcc aaaacctgtg ggctattccg
     7741 cgtttgaaca ataccgattc tcccgggcct gcatatttcg gttcttctcc caggagctga
     7801 taacccaatt tacccgatat cacctccagc atttcttcct gcttgaaatg ggtatgcatg
     7861 gccggtccgt gcccgggctg gacaaaattt tccagcagga ccctatcgcc gtccggctcc
     7921 tgtaccactt cccggaaaat gatttgctcc ccgatacagt tctctatgat gtgcggataa
     7981 ctgatcttca tttttttgcc gttttaagga taaaaataac taattttgga cattgtccaa
     8041 attcttcgga tgaatacaga agaaaaaatc ctggccaaag ccctggaaat gtttaacgaa
     8101 cggggtattg aatatgtcgg cctgcgtgaa cttgccggga tcttgggcat ccgggtaagc
     8161 aatatcacct attattttgc caccaaggac gacctggtca accggatttc tcttgaacta
     8221 agtgctgcca acgaagccgt attgcgcgca gaacgggaac ccggcctgca taccttcctg
     8281 gatacaatgc ggatcatttt taataaccac atgagatacc gctgcatcgc cctgagcctg
     8341 gtccacatcc tggatcagaa tgaggtgatt tccgaaagat ttaaaaaaac ccagctcgcc
     8401 cgccgaaact ttctgacgac cagcctctcc atccttgcag atggaggttg ggtcgccttt
     8461 cccgatcccg cagaccggga tgccctggca tccatgatcg ctctgctggc cagattttgg
     8521 atcccggatt caagatttac ggtgcataaa ctccagcctc cggaacaaat ccggtactat
     8581 ttgcatttat tggtggccct gttcaaccct tttcttactt ccaaggggaa aaaagaaacc
     8641 aggaaattca tagatgaaat cagtggcccc agtgacaaaa aatgaacacc taaacttgag
     8701 ctcccggacc tgctcaggcc atgacggaca ccatgtccag gacttgtttt cccagggcca
     8761 ggttgccatt aagttgtatt ccttgttgta tttccccagc ccgtatattt tttgaaaaca
     8821 ggctccaaca aaggtcggcg gatatgctta cttctgcgac cggtctgttt atttctgatt
     8881 tacccaggac ccaagccttc cccgaacgcg tcagatacca ggatccgccg gcttcgccgg
     8941 gtatatgtac tttaatgacc gtgccttccg gtgcataaac gtcgcggtag gtatacggca
     9001 aagcttgcat ataagtatca atgaaaggat aaaagaattt cctttccatg attccggggc
     9061 gcccggtggc ttcccggatc tgttgctgat ggtgccattt ctcggtgtat tccctggcca
     9121 ggtggaacca gtttttactt tcggtctccc cggcccaact gaccgcaaag ggcgcggtat
     9181 catagggttt cagggattga taaacatcgc aggtgagcgg cccggtgacc tcatgaagaa
     9241 ggatcaggac ctttgggcta agtcttttca tcgcctttac ccagtctgca tttaactggt
     9301 tcaggtattg gatcaattct tcgtaattgc ggatcacagg gctttccgtc cgatgactat
     9361 cccggagtcc ggacaggacc cggatattgg tgtccagcag atgggccgtt acatccttga
     9421 ccgtccattg ccgggcgagg gtctgcctgg tccattcagc cgggctgagg gagtgcagca
     9481 attcaatgag tttcaggtca agctcatgaa acaaatgcgc aacgggaatc ggtacgtctg
     9541 gattttcgtc caaaatcgtt aagctgtttt ttcaaggttg attcgaacag gaataagcca
     9601 gattttccat caaaaccctt atagatgaaa gacggcataa ccatgcatct ggtgagtgga
     9661 aaccgtggta aacctggata tggagatttc cacatccgga ttcacatgga cgggatcgag
     9721 tccgaatttc gctaccgatt gcccgcaacc ataaatatga atgcccgctt ctttcatttc
     9781 ccggagaatt tgaaggttta ggttgtccac cccgtatttc tttttgtagg tctcgttgtc
     9841 catgacgacg gcaatcgaag ggccccaaat cgctacggcc acgtgaagtt gattcttggg
     9901 aatgccgccg taaatatgga ggttgtacat cctggatatg tgctcaaggg gggcgtacaa
     9961 ctgcccgggg gcctccattt tttctcccag gtccacaatg attttgtaat cgatggacgg
    10021 gtccggttta tctttcccaa agggcacatc atacatggcc ccgaaatttt tgatggccgg
    10081 atgcagcata acctgggcgc tcagtccgga aaggaccaga ttgaagcaga ttaaaacaaa
    10141 cagatttttc atactattag gttttatggg ttaatgaatc acaagccccg aatttactca
    10201 ttttctatcc cgggctcaac agcccccttt ctcttttagt tttgaagctg tgcaatgtca
    10261 ttcaaaattt ccgtcagttc ggcgggcttg ctgaaaaaag gggaatggct ggtggccatg
    10321 ctgtacacct ttttgcaagg ggtttccgta tacattttcc gctggataaa gggagtgacc
    10381 gctttgtctt ccgtacattc aatataatac cgcggcaccc ggccatagtt tgtttcgctc
    10441 aactccaggg gaaccaatcc cgattccacc ggctcataac taagcaggag cttggccaat
    10501 tcggtaatct cctcagggca gtcggcgtac aaaccctccc ggtatatgga aggctgaagc
    10561 gtgtgtgcat tcaggtgccc gtggcgggtc acatagggtt ttagcattcc agcctggtcc
    10621 atggccgaat attcactttg agtcttaccg ttcgggatca ggtaagcggc caggtatacc
    10681 agtttttcga ttttatccgg ccggtattcc gcggcctgtg aaatcataat gccatttttg
    10741 ctgtggccga ccaggatcac cttcccctcg atttcatcaa tccgcctgca gatcaggtcg
    10801 accgtcgatt tcatccggac ctgctctatg ggtgttttat cccggcccat tccgggaaga
    10861 tccagggcaa tggcccggtg gccctcggcc tcaagaagcg ggatcacttt atgccagttc
    10921 caggatgaat gccaggaacc atgaatcagt ataaatgttt tcattgactt tgttttatga
    10981 tctatttatg aaaaagaggg agcctgttta gtccgggctg cttcccagcc gatcatggcg
    11041 gttttacggg tagggcccca atggtattgg ccgatcaggc ccgtggattg gatgacccgg
    11101 tgacagggga tcaggaaagc caccggattg ctgccaatcg ctgaacccac ggccctggaa
    11161 gccccgggac tttttacctg ttccgcaacc tgaccatagg tcgctaattt ccccatagga
    11221 atttttaaaa gcgcctccca tacttttaac tggaaatccg tacccttgag gtgcagcctg
    11281 atctcatgga gtttgttcca gtcgtggctg aatataaaaa gggcattctg ttggagcaga
    11341 tccgtctttt gagtgagtgc ggccctgggg aaatgatgct gcaattgatt ccatgcctgc
    11401 ttcccatcgt ccgcgaaagc cataaaacag atccctttgg tcgtggaggc caccagtaaa
    11461 ttcccaaaag ggctttcggc gaaactgtaa tttatggtga ggttttcacc cccgttttta
    11521 tattcaccgg gagtcatgcc ttcgatttgg acaaaaagat catggaggcg ccctgtgcct
    11581 gaaagtccgg tctcaaaggc ggcatccgac agggaaacct tatgggcttt cagcatggac
    11641 ttggcgtgtt caacgctgat gtattgaaga aattttttgg gactcactcc cgcccattct
    11701 ttaaacaggc gctgaaagtg aaacggactg agatggatct ggccggccac ttcatccagg
    11761 ttgggttgct ctttgaaatg gaggcggagg tattcaatgg cttccgatat tcgttgatag
    11821 tttaaatttt cagtgtctgc catttgccgt taattgtccc gcaaagatga attccggcgg
    11881 aaaagcggga aacccgaaac ttgctgaatt tacctttctc cggttttcat ttttccaaaa
    11941 attgatatgt attttgatct aatccgcgta cattcactcc aacatgaaat aaaccaaatt
    12001 tcatggcctt tacatcccct tttttgaaaa aataatataa aacccggata aaatcttttt
    12061 aacatgaatt cacgtcgcca ttttttacac aaactcagcg cttccctgat cgccctgcct
    12121 gcattttccg ggcgggaact gaatgaaatc caattgctaa atgaaaaggc ttatgaagga
    12181 cccgttttga gagtggccat cctgggcctg ggctcctatg ggaccagggt agccgatgcc
    12241 atgaagacct gcacccgggc aaaactggtc ggcgccgtca gcggcacccc ctcaaaaatc
    12301 aaagactggc aaaccaaata cggaatacct gaaaaaaact gctacaacta cgaaaatttt
    12361 gaccagatca aaaacaatcc ggacattgac gccgtataca tcattacccc caactccctg
    12421 catgcccctt ttgccatccg cacagccaag gcgggcaaac atgtcatctg tgagaaaccg
    12481 atggcgctca atgccaaaga ggggatgacc atgatcgcag cctgcaaagc ggccaacgtt
    12541 aaattactgg tggggtaccg cgtccatttc gaacccaaaa cccttgaaat catccggatg
    12601 agaaatgaag gcgaactggg gaaagttatg tttttccagg gcctctgcgg tttccggatc
    12661 ggggacccca gtcaatggcg gctgaacaaa gccctcgctg gaggaggctc catgatggac
    12721 attgggattt atgccatcaa tggatccagg tacctggttg gggaagaacc ggtctgggtg
    12781 accgcccagg aaaccaaaac cgacccggtt aaattcaagg agggagtgga cgagaccatc
    12841 acttttcagc tgggtttccc cagcggagtc attgcctcct gcctgtccac ctattccatg
    12901 aacaacctgg accgctttta cctgaactgc gaaaagggat gggccgaaat gcagccctct
    12961 accggttacg gcccgatcaa agccagaacc aataaaggag agcttacgca tacccacgtc
    13021 acccaccaga ccgtccagat ggatgaaatg tccgatatca tcctgggcgg aaaacagcct
    13081 ttagtacccg tagatggcga agaaggctta aaggacctga aaattattga cgctattttt
    13141 atggcggtga aaaccggcaa gaaggaaagt ctgtccctct aggatgggtg ggcttggatt
    13201 taaagcttcc tttttgcaat actgaacgcc cttgccgggt ttttttcaag gagcaggtcc
    13261 aggtcttcgt ccctgaaacc tttcgctttt aatgccggga tgagatctct gaaaatggcg
    13321 gtgtaagccc tgatgttttg atccgttttt ccgggatcgt accacccggc atcatgagag
    13381 atcaacacct ggtccagaaa accctgttcg cgcgcataaa ccagcttgtc cacatggttt
    13441 ggcagttccc agcccaaacc gtcaaaactg atccagcatc cagaagctgc agcccggaga
    13501 tagttttgat tctctttttc gttttgggca tgtacccata tgaatgcgcc ggcagaaaca
    13561 cggctttcct ccaggatttt taactgtggc cacaaaccgc gtgcttcccc ggtatgggat
    13621 gcaatggtaa gccctgtctt cagatggcaa attgcggccg cctttaccag tttttcctgc
    13681 atgggatgca gaggatctgt agcatcgacc ccgattttga tgaagcccgg tttcaccgtg
    13741 gtaccctcga tcccgtgggt ccactccccg gtccatagat cagccaatgc ttcgggttcg
    13801 agggcaaaag caaaagcggg tatgtatttg ttttggactg ccccgtaaaa ccccgtattg
    13861 gtgagaattt gcattccggt cttttgagct gctttttcaa gcaggcggac atcccggccc
    13921 aaataggcgg gggtggcatc cacaaaataa gccacccggt ggtcacgcag ctcctgcaaa
    13981 aacggcaggg tcgcctgtaa aaccgtatcg tgattccaat ggggatccag gctgtcggcc
    14041 ccggtaaaat ccaccaggat atgttcatgg gtgagccagg tcatggtgtc ttcaccagat
    14101 accttaccca ggaccgtttg aatcataaaa ggctcatgct ggcttttgca ggaaatacat
    14161 aaaataccgc aaatcatgca gaaccttgaa aattggaata agattcctgt cattcccggt
    14221 ggcagccttt tattatatac gggcattttg taatctgaat tcaggatatt ccagtggcag
    14281 ctgctggtcc tataaccagg tttccagcac aaacatgaat ttaaatggac tccagttgtt
    14341 tacgggtgaa atcatgattt cggtcgccgg tattcagcgt tgaagaaggc tcaaacaaca
    14401 ggatggcaac ttccttacgc gcaaacggtt tatgttcgat accgcggggc acgaccagaa
    14461 attcattttc ccggacggtg acgatgccgt cacggaaatg catttcaaat tctccgttaa
    14521 ggaccatgaa taattcgtct tcatgatcgt gtttatgcca aataaattca ccttcgaatt
    14581 tgaccagttt gacctgctgt ccatttagct cagcgatgat acgcggatta aaataatccg
    14641 aaaattgcga aagtttatga gcgaggttaa caggggaaag gggcatccgg aatattcttt
    14701 actggccctt taggatcaag tccttttggc gatcatctga tccacgccgg caataaaatc
    14761 tttggcggag gccatatacc ccgtcttgcc cagggctttg atattgtatt ttttggttgc
    14821 tttttcctgc tccaggtcaa aaacccagac ggtaggatac ccggtgacct ggaaagcctg
    14881 ctggagggaa tagttctgat tgcggatctt ttccggaagg gttttccggc gcgggaaatc
    14941 gagctccaga aggaccactt ttttggccgc ccattttttg aaatcttccg tcaggaaaac
    15001 cgcagcggaa aggcgtttgc accaaccaca ccagtcgctg cccgtaaaat tggccaggat
    15061 gggtttaccg gttttttggg ataaagcgta ggcttcatcg atgttggtca accagccttc
    15121 gtgggtcgcc ttgtattccg actgggccgg caggtcccat gaaagaagga ataaggaaaa
    15181 caaaagggaa aaaccgaaag tcagggaacg cgttttcatg caaactataa ttatatcgcc
    15241 gtcaaaaata atggtttctc cggattgtgg tctttcaaat agccgggcga attgattaaa
    15301 aacaaaatct taaatgaagg gattgaaatt tatgaggtat ttaacatccc ggtaaatatt
    15361 ttccttccgc ttagtttata attttagtaa cctaactgct ctcccatgaa aaaatggttt
    15421 tttattttaa ttcttctgaa tcttcattac ctgttcgcgc aggccccgat tggcggccga
    15481 tgtgaaggct gtgaggccgt atttgaaaac ccggttcctt ttaaccaatt ggaccatgag
    15541 gtctatttac cggactggaa tgacccgggc ccccggatgg tcatacgggg aaaggttttt
    15601 aaaaaagacg ggaaaacccc tgcggagggc gtggtcctct atgtctacca caccgaccaa
    15661 aaaggaatat acccggtgag ggaccaggat aagggatggg caaggcggca cgggtacctc
    15721 cgcggctggc tgaaaaccaa cgcccttggc gaatatgcca tttatacgct gaatcctgcg
    15781 gcttatccca acgaacgttt cgcagcacat atccatgtgt ttgttaaaga accggataaa
    15841 aatccctatt acattgacga gttcctgttt gaaggggacc ccttcctcac cgaaaacgaa
    15901 atcaaccctt cccgcccaag gggaggcaag ggcctgatca gcctttcaaa acaaaatggc
    15961 ctgctggcag ggaaaaggga tattacttta ggcctgaaca ttcccggata tcattgattt
    16021 cagtcgtgca agcccgaata atcctatgca tcctggcagg ggctttgacc caaaggagcc
    16081 cggcccatcc cggcccgggt atagtcaaag acagcaaggg gaatatttat tataccgatt
    16141 taacggcggt atggaaaatc aatccttcgg gtataaaaac aaaggtggta acagggattc
    16201 atacccatct gctgcatatc gattcccagg acaaccttta cggagagcac ctttggtaca
    16261 acggagagca cgccgatacc tggggcagtt atgcctggcg cctttctgcc gacggacggc
    16321 tggataccgt actcggaccc gtaaccggtt tcctggaagg cctattttca ttcgaccggg
    16381 accgttggga aaatgaatac tgggttcagc gtggaccggt taaaaaattt ttccgaaaaa
    16441 agccaaatgg gaaaattgaa aaaatcgggg aggcccgtat ggaaaatccg ggttggatca
    16501 aggtcagccc ggacggacgg gtttattttg tggccggaaa ccacctttac caactcgata
    16561 ccaccggagc gttttccatc ctaagtcgaa atatcggatc cataatcctc acccgccctc
    16621 caaccagcga tccctacgtc gccggcttgt ggattcaccg cgaccgcatt tttgtggccg
    16681 attatacggg gagacaggtg aaagaagtcg ggatgaacgg tacggtccgt gtcgtggccc
    16741 gctcggaacc gccctggtcc ccggtaggtg gaatatttga caaccaggga tacctctggt
    16801 tgcttgaaaa caacgaaaac aatacggtaa atgtgcgaaa aatcaggaca aactgatttt
    16861 taacccgcgt gttattttat accgattttt taggtcttcc tcttcttttc ttttcagtgc
    16921 cgaccacaag ggtaatggct ttggcgggac ggccgcgttt ttttgccaca agggctgttg
    16981 caggcatcac tgccttagcg ggcctccctc ttcttttctt cacaggggtc gctgcaggtt
    17041 caaccgcttt ggcgggcctc cctcttcttt tcttttcagt gccggccaca agggtaatgg
    17101 ctttggcggg acggccgcgt tttttctttc cagccccgat ggccggtaaa gcggctttgg
    17161 cgggacggcc gcgttttttg aggattggtt tggaatcgga aggtttaagg gattctgcat
    17221 atttattgtt caggaaggtg ctgattcggt gtgcggccga tccgtccagt ccgtcaagca
    17281 gatctacaca gatcgataat acattgattt catcggaatt cgccatcgta ggaaaattta
    17341 gtgattagtt atcggttaaa aaacaaaaac gcaaagttac cggaaatacc gcaaataaaa
    17401 ccgcataacc tgaataataa tgcaacttta aaaggcacaa cgggcgatgt atgagttcaa
    17461 atcaatacca ataaaaaccc atttcttttt cattgaaatc gcagtggttt ctttcaacca
    17521 ttgttaggcc tggtggcatc agggaagcaa gaccgtctgc cgccggccac cgcggatcct
    17581 gagtaaaatg gcctttaccg caaagcgtta ccttggcatc ggcctgctgt ggtaaaaagg
    17641 cataacgccg caggcagata agccctaaat ttatatggcg aatggctgat attaccgaag
    17701 aattgatcat aaataaacgg aaccgcagtg aatgaaattt aatttaatat ctattttgca
    17761 tcggttaaac attgctgacc gcaatcagtt ttttgaatga gtccgatcat caaggataaa
    17821 aatgtcccga atgaaatttg tcatggctga aaaaacagta ataaaatcct gatatatgaa
    17881 aacttcaatc tttaattttc tcctggcctt gttttttata agcgggctct atcttgtttc
    17941 ctgccaatcc aaaggcgtca agtcggacac tcctcctttg gatgttcagg aactgaccaa
    18001 aaaccaacct gaaacccacg gcgccgaaat aaaggagtcc gatatcgtcc ttgccactcc
    18061 gcttgaccag gccatggcca ccaagggaaa tgcgacctat gatgtcaaat gtaaaagctg
    18121 ccatcgcctc tccgaagaac gactggtcgg accgggctgg aaaggggtga ccaaattgcg
    18181 caagccggtc tggatcatga acatgatcac caatgtggac atgatgctcg agaccgatcc
    18241 tgaagcccag aaactgctgg agcagtgtct ggtacgtatg cccaatcaaa atgtgaccct
    18301 gacggaagcc agggagatcc ttgaattcat gcggaaaaac gacggagaac tctgagcctc
    18361 ctccgtttga tcagatgtag taatcattca ataaaactta tcaaatatga cttcgaataa
    18421 attcttcacc aaatcggcgc tgttgatgct gattttgatt acctggtccg cctgcaagcc
    18481 cagggggatc cagcaggcta tcagttcgga tgcctctggc aaagtttatg tggcgccagg
    18541 cacccatgat gaattttaca attttgtatc cggcggcttc agcggccaaa tgtctgtcta
    18601 tggcataccc tcaggcaggt tgttcagggt cattcccgtg ttttcagtag atcctgaaaa
    18661 aggttatgga tacagcgagg aatccaaacc catgctgaat acctcgcatg gcatggttcc
    18721 ctgggacgac cttcaccata tagccctttc cgtaaccgat ggcattcacg acggacgctg
    18781 ggtttttgga aatgccaaca ataccccgag aattgcccgg gtggacctga ccaccttccg
    18841 cacggcagag atcatcgaga tccccaacag cgccgggaac cactcctctc cctttatcac
    18901 cgagaattcc gaatatgtgg tagcgggcac ccggttcagc gtacctatgg acaatggcag
    18961 cacggatgtg ccgatcagca cttataagga taatttcaaa ggcacggtaa gttttgtcaa
    19021 agtagaccca agctcaggcc gaatgagcat tgccttccag attcttctcc ccggggtcaa
    19081 ttttgacctt tcccgcgccg gcaaaggaaa atctcacggt tggtttttct tcagttgtta
    19141 taattcggaa caggccaatt cccttcttga agtcaacgct tcccaacggg ataaggactt
    19201 tattctttgt gtaaactgga aaaaagcgga agaatatgtg aaccagggca aaggcaccaa
    19261 gaaaaaagtc aggtacgccc acaatttcta tgatgagaaa aagcatatgg gcaccagcac
    19321 tatggaaacc gaagtcctgg tcctggaccc gaaagaactg gccgatatgg tatatatgat
    19381 gccctgtccc aaatcgcccc atggctgcga tacagacccc agtggggaat ttatcgtggg
    19441 aagcggcaaa ctggctgcga ccatgccggt attttccttt accaaaatac agcaggccat
    19501 cgcggataaa acctatgacg gggattatga aggcatcccc gttctcaaat acgaagccgt
    19561 gctccacggg gaagttcaaa aaccgggatt aggccccctg cataccgaat ttgatgccaa
    19621 cggcaatgcc tatacttctt tctttgtatc ctccgaggtg gtgaaatgga atgtgcaaag
    19681 cctccaggtg ctggaccggg ttccgaccta ttattcggtg ggccacctaa gtgttccggg
    19741 cgggccgacc aaaaaaccct ggggaaaata catgatcgct tacaataaga ttaccaagga
    19801 ccgcttcctg cccacaggac ctgaactttc ccaaagcgcc caactgtatg acatcagcgg
    19861 ggataaaatg aaactgattc tcgactttcc caccatcggg gaaccccact atgcggaagc
    19921 catcccggcg gagctggtag ccaagaacag cgttaagttt tacaaaatcg aggaaaatga
    19981 aaatccctgg gccgccaaag gagatgattt tgcccgcgtg gaacgcaaag gcaatgaagt
    20041 ccatgtttac atgaccgcca tccgttccca ttttgtaccg gacaatattg aaggggttaa
    20101 attgggggat gtcgtctatt tccatgtgac caatatggaa caagactggg atgttcccca
    20161 tggttttgcg gtcaagggag caaacaatgc ggaactgctg atcatgcccg gtgaaaccca
    20221 gacactgatg tggaaacccg accggatcgg tgttttcccc atgtactgta ctgatttttg
    20281 cagcgctttg caccaggaaa tgtccggata tatccggatc agcgcacccg gttccaacgt
    20341 gcccattaaa ttcagcaccg gggatctcgg tcctgaacaa taggccgctt tgtattcaga
    20401 atctttaaac ctttacatat gaaaacgatg gtttttatta tgatagtcct tggtatgcta
    20461 tcctgtaaga ataccacaac tccggattct accgtcggtt cctccgcgga agcccccgcg
    20521 gcgggccagg ccaacgtggt ggacgacgaa tccatgaagg atgtggtcaa aatcgcggtg
    20581 ggatcccctg accatactac cctggtcgca gccctgcaac aagccgacct ggtaaattca
    20641 ttggccaatg cggggccctt caccgtcttc gcgccaacca acgcggcctt tgacaagttg
    20701 ccggccggca cggtggacgg cttgatgaaa ccggataaaa aaacggatct tcaggacatt
    20761 ctgcagtacc atgtgactac ttccgcactc aaaacctcct tctttaagga cggcatgacc
    20821 ctcggaatgg taaatggagg caatatttct tttcaggtca aggacggaaa aatcatgatc
    20881 aacaattccg caacgatcgt ggcttccgta ccggcatcca acggcatcgt gcatgtgatc
    20941 gatgccgtcc tgttgccacc tgccaagtaa accgggatct ttgccaaagc ctaacacaaa
    21001 cgacctgtta tgaaaaacaa caatctgacc gttttttcca ggattgtcat ggccttcgcc
    21061 acaggcatgc tggtagccgt attctttttg ccggcctgga gaattgatct tttcgccccg
    21121 caatatcccg aaggtctgaa gatgaatatc tggctggacc ggttgaccgg ggatgtggac
    21181 attatcaacg gtctgaatca ctatatcgga atgaaaccct tctccaatga atccttcccg
    21241 gaattcggct ggttgaaata tgtcgtttat tttttcatag caatgggttt gttgatttcc
    21301 ctgaccggga aaaaaaggct tttgatcctg tatctgatcc tgaccgcaat cggtggaacg
    21361 cttgcgatgt atgattttta caaatggggt tatgagtttg gccacgatct ggatcctaaa
    21421 gccgccatcc aggtccccgg gatgtcctac cagcctccgg tattcgggca taaaagattg
    21481 ttgaatttcg attcttattc atttccggat atcggcggct ggctggtcat ctcctcggcc
    21541 atcctggctt tcctgatttt agtttatgaa tggtacctcc accgaaaacc aaaaacgaca
    21601 tccccggttg cggtcctgtt gatcaccgga gccctgtccg ccaccctttc cgcctgccag
    21661 gtcaagccag aacccctggt gtcggggcag gatatgtgtt ttcattgcaa aatgaccatt
    21721 gtcgaacccc gatacggctg tgaaatcgtg acccaaaagg ggaaggttta taaatttgac
    21781 gacttctcct gtatgaaatc gttcatccgg tccaacaccg tgaaacagga agaaatccgg
    21841 tttaccctgg ttgccgactt caacaatgaa aaggactggc tccccgcgga aaccgcaatt
    21901 tattataccg ggcctttgat gaaaagtccc atgggttcca ataccggggc tttcccggac
    21961 caggcggaag cgtcccggtt caaaggtgaa aacgaaggca ccctttccga ctggaatggc
    22021 ctgatgcaaa gccagtgatg agcaggctga tctttttctt ctggctcttg cctgccggcc
    22081 tggcggcaaa agtgacagac ctctatcccc atgaggacag cacctcgatc cataaccatt
    22141 ttaaagaagc ccgggatggg gacacgctgc ggtttaacga gggggtttat tatatccaca
    22201 gcctggtggt cactaaaccc ctcgtcattc tgggccgcgg gaattgtatc ctggacggct
    22261 ccggtaaata cgaaatcctg accatcagcg cccgtgcggg aaccgttcag ggattgaccc
    22321 tgagaaattc cggttattct tcaatgaatg attttgcggc gctcaaagtc attgatgcgg
    22381 ccaatttgct gatcaccgac aactcgatcc tggattccta cttcgccatt cacctgtcca
    22441 atgcctcccg gtgcaccatc cgcaacaata ccattgaagg aaaagccata tccgagcaat
    22501 tgaacggcaa tgcgatccat gcatggaaaa gccaccattt gatcatcgaa aacaataccg
    22561 tcagccggca ccgggacggc atctacctgg agtttgtcac ccattccagg attacgggaa
    22621 accacagtca tctacagatg cgctatggcc tgcactttat gttttccaat gatgatgtgt
    22681 acacccgcaa tatttttgaa gaaaacgggg caggggtggc cgtcatgtac agcaaacggg
    22741 tcaccatgaa agaaaacctg ttccggaaca attggggccc ctccgcttac gggctgctcc
    22801 tcaaggacat ttcggacagt gaaatcaccc aaaaccgttt tgaagaaaac acccgggcca
    22861 tccatatgga agggacctcc cggatccaac tccgtcaaaa tttattcaag gacaacggct
    22921 ggggtgtggc gatccaggcc agttgcaatg aaaactcctt ttcaaacaac aattttatca
    22981 acaattcctt tgatgtttcc accaatggcc aggtcatcct gaacgtattt gaacacaact
    23041 actgggatga atacgagggg tatgacctca acaaagacgg caccggagat attccttacc
    23101 atcccctcag cctttttgga ctgctgattg aacaaaatcc cagcatgctc atcctggtaa
    23161 aaagttttat gatgcaattg tttgaaagaa tggaaaaagc cattccgagc ctcactcccg
    23221 aacattttgt cgatcttcaa cctttgatga aacccttcac gccatgatcc ggatcgaaca
    23281 tatcagcaaa gaataccgca ggatccaggc gctcgacgac atcaaccttt ttttcagccg
    23341 cggccaggct gtttctttga tcggccctaa cggttccggc aaaaccaccc tgatcaaatg
    23401 catcctgggg ctggtgctcc ctgaccatgg acgcatcttc gtccataccc aacccattga
    23461 aaccagccct gcctacagaa accagatcgg atacatgccc cagatcggac ggtaccccga
    23521 ccagatgaaa gtcggccagc tctttaaact gctcaaggac ctgcgtcatt cggatcctgc
    23581 aaaactggat tacgaattgt acgaagaatt taatctttcc aatttgtttg aaagccgcat
    23641 gcataccctc agcggcggca cccggcagaa agtcagcgcc gccctggcct ttttatttaa
    23701 cccggaaata ctcattctgg atgaacccac cgcgggactg gaccccttgt cttcggaatt
    23761 gctgaaaaag aaaatcattg ttgaaaaaga aaaaaacaaa ctggtcctga tcacttccca
    23821 cattctcagc gacctcgaag acctgtgcac ccatgtggtt tacctgcagg aaggcaaact
    23881 gatcttttac aaagacctgc ccaccctcca gcaggaaacg agggaacaga aactcagccg
    23941 ggccatcgcc cattttatga aaaaaaacaa tccgatagct cccctgaatt aaacccccat
    24001 gcttaggtta ccccgttata ttatttatga tatcctccgg aacaagatca tccttgccta
    24061 caccctgctc cttttcctga tctccaccgg tttgtttcag ctggaagaga acaacgacaa
    24121 ggctctgctg ggccttttga gtgtggtgct cattgtcgtg ccgctggtaa gcctgatttt
    24181 cacgaccatt cattattaca attcttatga atttatggaa ctgctcctct cacagcccca
    24241 aagccgctgg agcatcctgc tgagtgaata ttccggagta gccgtttccc tgctggtagc
    24301 ctacgggacc ggggtgggcc tgccactcct gatttattcc ccggggccga caggctttta
    24361 cctgctgctt gccggtttgt tgctttcatt gatcttttgt tcgctggcct tcctgatcgc
    24421 cgtcctgtcc cgggacaaag ccaaaggaat cgggatcgcc ctgcttttgt ggttctattt
    24481 tgccctgatc tacgatgggg tcctcctgct cctgcttttt tcattgagtg actatcccct
    24541 ggaaaaactc acttttttat tttccgccct caaccccatt gacctcggaa gaatcttcgt
    24601 catgcttaaa atggatatcg gcgcactgat gggttacacc ggtgcggtgt ataaaaattt
    24661 cctgggcaaa gaaaccggga tcctgtttac ctgcctcgtc aatttggtct gggtgatcgt
    24721 acccctgctc ctggcaaaaa aaatatttaa taaaaaagat atgtgatcac cggggcgcgg
    24781 tcctcatcgg ccatctccgt ccaggaggtt gcccaggccg cccaggatgc ttccttcatc
    24841 ctttcttttg accgtcccat aggccaggat acgggaggcc aggcggctga tcggaagggt
    24901 ttggatccat acagagcccg ggccttcaag ggtggcaaaa aacaaacctt ctccgccaaa
    24961 aatggtgttt ttgatcccgc cgacaaactg gatatcataa tgcacggagg gggtgaacgc
    25021 aacgatgcat ccggtatcga tttttatggt ctccccatat tgcagttccc gctgcaggac
    25081 atggcccccg gcgtgaacaa aagccatgcc gtccccttcc agtttttgca tgataaaacc
    25141 ttcgccgccg aataatcccg tgcccagttt tcgctggaat tctatgccca cttcgactcc
    25201 ttttgcggcg cataaaaaag catctttctg gcagatcatc ttgcccccga gccggtaaag
    25261 gtccagggca ataatcttac cgggataggg cgaggcaaaa gaaaccctgt ttttgccatg
    25321 ccccatattg gtataggccg tcatgaacag gctttcgccg gtcaggaccc ttttgccggc
    25381 cgacatcaac tttccgaaaa atcctttgtc ttcatgacgc ccatccccga aaatggtttc
    25441 catctggatg gcctcatcca tcatcataaa ggcgccggcc tcggcgatgg cggtttcccc
    25501 gggatcgagt tcaatttcca catattgcat ttcttccccg tatatcttat agtctatttc
    25561 gtgatccgac atcatgtttt ttttggggca aagataataa ggggagggga tcgaaacccg
    25621 gatacggaat aggccatcca ccttcatatc ttcgttccat gaaaatccga tccatccggt
    25681 tccgactgga aaatcttcag ctctccaaac cctatacgat cgcctggaaa accgtggacc
    25741 aggtggagaa ccttttttgt tttctggaga cggaccaggg cattaccggg tatggcagct
    25801 gcaatcctga aaacgaggtc gtgcatgttt ccaccaagga tacccttgag gccatggacc
    25861 tgtttgaccg ggacttgttg ctgaataagt cattggaccg tcccgctgac ctgattgaat
    25921 ccttgttgcc ttccctgcac ggagtcccca ccctgcttgc agccctggat atcgccgtct
    25981 gggatgcctg gtccaaagga ctgggaaaac ctttggtgga agcactgggg aaaaaaatcg
    26041 gatcccttcc tacttcggtc acgatcggga tcatggagga cgatcagacg gtaaaggaag
    26101 ccaaagaatt ccttgaccgg ggtttcacgc acctgaaaat aaaaatcggc ctcgaccccg
    26161 aaagggatgc cgcaaggttg gtaaggctca gaaaggagct tggccccggc ccttttatcc
    26221 gggtggacgt caaccaggga taccgcctgg aagattttaa gaaattgtac accgcctgtg
    26281 ccgaattgaa tattgaattg tttgaacaac ccctgcgcac ggattggttt gatcagatcg
    26341 acgccctgcc ccgcgaggtg aaatcctcca tagccgccga tgaatcgctg cacggggaat
    26401 cggacgcaag gcagattgcc tctgccggac gctgtggcat ttataacatt aaactgatga
    26461 agtgcggcgg actgcatcag gccggcaaac tcgcccggat cgcggagacc cacgacatca
    26521 acctcatgtg gggctgcaac gacgaaagcc ggctcagcat tactgctgcc ctgcacctgg
    26581 cttatgcctg cggcaggacc cgcttcctgg acctggatgg cagtttcgac ctggcccggg
    26641 accccgcttc gggcggattt gacctgattg acggaaaact gatacctttg gaaagacccg
    26701 ggctcgggat ttttatggac aatgactaat cctcaacatg gaatccttta atacgctgat
    26761 cgaagccatc caggcataca aggaagaagg ctataccgaa gattttaacc ttcaggaaaa
    26821 ttgtatggtt tgctccaacg gaaaatacaa actgaaggcg ggtgattttg taatcgacaa
    26881 acattaccgt tttgaggaca ataccgatcc ctccgaccaa gccattatct atgccatctc
    26941 ctcggacaaa tacgatgtaa agggcattct ggtcaatgct tatggtattt acagcgacga
    27001 catggcaagc gaaatgattg aagcgctatc aggcaggaag tagatgttat cagtttatca
    27061 gttcaacagt tcaacagttc aacagtttat cagaggcttc tttccacgcc caattccaat
    27121 ccccttaatt ccgccagccc cctcaacctg ccaatggcgc tgtaacccgg ataggttttc
    27181 ttattcaggt cgtccagcat ctggtgtccg tggtcgggcc gcatggggag attcgtcttt
    27241 ctgcggcgct gaagcaatac gatttctttc atgatggcat acatatcggt atcgccggcc
    27301 aggtggtccg cttcaaaaaa atttccttcc ggatcccggg cggtattgcg aaggtgcagg
    27361 aaatggatcc ggtcgccaaa acggcggatc atggccacca ggtcattgtc ggcccttgca
    27421 ccataagaac cggtgcaaaa acaaagcccg ttggacgggg agggtacggc gtccagcaat
    27481 tccgcggcgt cctcttcggt gcatacaatc ctgggaagtc ccagcacggg ataaggcgga
    27541 tcatcgggat ggatggccat tttgacccca cgctcttcgg ccacggggac tatctgccgg
    27601 agaaaatatt tcaggttttt tttcagggaa tcccggtcga tgtgatgata ggactccagt
    27661 aaggccaata cttcagcagc ggtaaaactt tcggtacttc cgggcagtcc ctggaggcaa
    27721 ttgtggaaaa cccggctcct tttttcttca tccgcctctt cataccatcg ttttgcacgg
    27781 ctgatctgat cttccctata gtcctgttcc gcgcccggcc ttttcagcat aaacaaatca
    27841 aaagccatga aagccggttt ttcgaatcgc agggccttgc tttgatcggg catcgggtat
    27901 tccagatcgg tgcggatcca gtcaagcacc ggcataaaat tataagtgac cacttcgagt
    27961 ccttccgccg ccacatttcg gatgctggtt ttataatttt caatatggcg gagataatcc
    28021 ccggatccct ttttgatgtc ttcgctgacc ggcaggcttt cgatgacttt ccaactcatc
    28081 ccggcttcct caataaggga tttccgtttg cggatctcct ccggggtcca gatttctccg
    28141 acgggaatat ggtgcaaggc cgtcaccacc cccgtgcaac cggcctgccg caggtcccaa
    28201 aggctgacgg catcgtgggg gccataccat cgcatcgttt gaatcatact catggatgca
    28261 aacatacatc attccgccgt ttcgcaatcc ccacaggctg cgtccaggtc cggataaaaa
    28321 agattggttc ggatccggtc gtgtacagaa ggcttttcat ccccataacc gaaaatcagc
    28381 gccagcatga ccttttcctt ttcgggaatt ttcaactttg catatccctt cgttccctgc
    28441 acatacttac ccagaccgat ggggcaggaa tccagcccca tggatttagc cgccagcatc
    28501 atattttgag cacacatgcc gaggtccagg caggcccatt cattgtcctc aggagcggta
    28561 agaaaaacca ccacaggagc tccgtgaaaa atggggtttt tatttttgag gaaatcaatt
    28621 ccatgcgcca aatgcaaaga acgggtcgct gatttgatga tcgttttaaa tcccgatttt
    28681 aagattcctt tggcagccga cttgagcact tccttttcca agccggcgat gtcctccttt
    28741 ttatcaacta cataaaacct ccacggctgg cggttgattg ccgagggcgc catacgcccg
    28801 gcatccacta tggcttcgat gtccttccgg tctaccgcaa ggtccttata cttgcggacg
    28861 gctctgcgtt catagatcat cctcaggatt tcgttctgat tcagggttcc cgggggtgct
    28921 gtggtttcta ttgtcttcat gaccaaaatt tgacgtaatc aatttaccaa aatccgaagg
    28981 agggttttat gatcccgatc aggaggccga ctagctgggg cagccgatga ccttcaaatt
    29041 gaatcggcct atcccccaaa acgcctttat ccttaggggt tatatttcgt tacttttagc
    29101 gtcccgaata ggaaacttat tttaattatg gtgaaaccac ttctataagg tatatatgct
    29161 tttgacaggt gcacgaatga gtggggtccc ttacctcagg cgctttttca ggcctgtttt
    29221 tccacggcga attccttctg atatatttta acgagcagga aaaaataggc tatcagtaag
    29281 gggccaaaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttctgatata
    29401 ttttaacgag caggaaaaaa taggctatca gtaaggggcc aaagaccagc cccggcaatc
    29461 caaaccactg aagcccgatg atgactccca atatggtgat cacggggtga acgttggcaa
    29521 accatcgggc aaagatcagc ctgaaaaaat tgtccatcag ggaaataatc aaagtaccat
    29581 aaagcaagat tcccaatcct tgccaggatt ctgcggtcac cagcatgatg atcccggccg
    29641 gcatccagat cagggctgcg cccaccaccg ggagtattcc ggaacaaccc gtgagcaccc
    29701 cccagaacag cggatccttc atgccaaaca accagtatcc gaaagtggat acaatgccct
    29761 gggccagggc gatcatgggg accgcaatcg cattggcctt ggtcatgtca tcgagttcct
    29821 gggtgagcat ttgaatgcct tccctggtaa aaggaaggta tcgcgataaa gccggttcga
    29881 tatgccccgt attcaccagg agataataca ggaggaagta catcatgagt aaacttccca
    29941 aagcaaggat ggacatattg aggatggcag gaactgaatc ggccaggccc cggatatact
    30001 ccaccagggt atcgctgctg aataccggtc ggtcaaaaaa ccggttgatt ttcaacaaca
    30061 acgcgttgaa cccctgcatc aggtcctgcg gattgctcag ggtggtttgc aattggccaa
    30121 tcaataaata caccaggcca aacaaaggaa tgatgatcaa taagaaggaa atgaagatga
    30181 tcagggtggc cgccatccac cttttccagc gataaaccat gaccagcctt ttcataaatt
    30241 tcctgaacaa gacataaaag atcagtgcgc cgagaaagga tacgataaac tcgtttaaac
    30301 tgtaaaggag gaaggccatg atgcccagga tcataccgat cacaacgatt tgccggcttt
    30361 taccgggata caaactggat gaatcaggca tgaacggatt tacgggttgt ccatccaaat
    30421 ttaggatttt aaaacagaac ggtggttagc ccggtcaaac cgggtttaag tcctgcggca
    30481 ttctttgagc cgccggggaa gtgaggctgg acgccaggtg ctcgatcttt tcaagcaatt
    30541 gcggcatgac cctttcgatt tccgggctca ggctgacgcc ttgtagctgg atgttctcga
    30601 tgctgaccac aaataaatga atatcgggca tcttacccag gagttgcaat gcgcccatca
    30661 tatccttcag tccaatatca tgcgtactca tggccttggg aaaatcctga gcgaatcggg
    30721 gccggatgag gcggatggtc ccgggcggat gacggtccag cgtagcatcc accaggataa
    30781 tggtttcata ttcttccagg caacccaaca ggtgaaaccc ccctgtgccc ccatccagca
    30841 cctcaacgcc cggaaaccgg ttttgggctt ccagcctccg ggcggcatgg accccgaccc
    30901 cttcatcgcc catgagataa ttcccaatac ccaggatcaa aatacggttt gaggaggtcc
    30961 ggttcataag gctttactcg gggtttgggg tatgggtatg ttccttttca aatgcctctt
    31021 cttctataaa tttccaacct ccccccatgc tgctgatctc tccgcgtccc tcgatataat
    31081 cgtgataaaa caccagataa atatggatca ccacaaaaag gataaaaaac cacatggccc
    31141 agtgatggat ctgacgggtc aggatatccc cgccaaacaa agcgggtacc caggcaaaaa
    31201 gttgcgggaa ccaccagtcg ctcatggcgg catacaagcc aaaaccggtg aggcattgga
    31261 tcagaaaaag aataaaagtc agaaaatagg taaagccggc aagtgcattg tgaccgatgc
    31321 tcatgtgttc cctgtttttc atcatcagca catccatttt aatcaccgtc cacatttctt
    31381 taaaaaacgt tcctgaaacc gggataaact gtttccagtt ggcaaattta ttaccgacaa
    31441 atccccaata gatcctggcc aggaagttga aaaaaaacaa atatgcagcg gcaaaatgaa
    31501 tataccggat ccagcccata gtgaattttc cggtagcctc ccgctggctc tgtaaagcca
    31561 atggatcggc gatataaaaa ccggtggcga ccagggcgac catgaccagg gcattcaacc
    31621 agtgataaaa ccgcaccggc aattcccaaa cataaaccct gcgaagcatt tgaattttca
    31681 aacctttagg ttccatacat atcggtttaa acttattcgc cgcccactac actgatccgg
    31741 ctgatgtgtt tgccttcctc gtcataaagg tggacactgc aagccataca ggggtcaaaa
    31801 ctgtggacgg ttctcaatat ttcaagcggt tgtttttcat ctgccaccgg ggtattcaaa
    31861 agggcggatt catatgcgga caactggccc ttgccgtccc ggggtgaggc gttccaggtc
    31921 gtagggacca ccaattgata gttggcgatc ttctgatctt cgatattgat ccagtgggca
    31981 agggccccgc ggggcgcctc cgaatagccg accccgagtg cctgggccgg ccaggtagcc
    32041 ggatcaaact tgctcatttc ggccatccgg gtatccccgt tctttatatt ggagatcagt
    32101 tgatcaaaga actcaagccc ccattgcgcg atcaatacac tttccagccc gcgggccgcg
    32161 gtccttccca gggtagagaa caaagcagtc accggtacct tcaaatcggt cagcgccttg
    32221 tccaccaccg ctttaaaatc ttcccttccc cttgcatagc ccaccaatac ccgggccaac
    32281 ggaccgactt ccatggcgtg tccgttccag cgcggcgttt taaggtaact gtattctttg
    32341 gcggtgtcca ggtgttcaaa gggtggtttt ggcccattgt aattcacctt gctttctccc
    32401 ttccaggggt gcaggcccac ggttttccct tcacggtatt cataccagga attggtcaca
    32461 aactcctgca cttcctcatc cttgcgaaga tcgacctcct ggatgttgcc gaggtcttta
    32521 ttcaggatgg cgcccgccgg aaacttaaac gaggccatat cgcggaatcc gtttgtgggg
    32581 aggtccccat acaccaggta atttccaagc ccgcctccga tcgctcccca gtctttgtaa
    32641 aaagaagcga tggccatcag gtcagggaca tatacctgtt cgacgaattc ttttgcatcc
    32701 tgcagcaatt gcccgaccag ggcaaggcgc tcggcgttga tcccgcttac atcgtcgatg
    32761 ccgatggcgc aggccatgcc tcccaccagg taattcgggt gcgggttttt cccgccgaaa
    32821 atggcgtgaa ttttaacaat ttccttctgc cattccaggg cctccaaata atgggcaagc
    32881 ccgatcaggt ttacttcggg aggcagtttg tatgccggat ggccccagta tccattggca
    32941 aaaatgccga gctggccgct ttccacaaat tttttaatgc gggtctgaat atcactgaaa
    33001 tagccgggtg aacttttggg ccaatgcgaa atgctctgag ccagttcgga cgtttttttg
    33061 gggtccgctt tcaaggcatt gaccacatcc acccaatcca tggcgtgcag atgatagaag
    33121 tgcaccacat ggtcatgcat gtactgggtg caaaacatga tgttccggac catctcggcg
    33181 ttgggcggca cgacaatatc gagcgcgtct tccaccgccc ttacagaggt cagggaatgc
    33241 acggaggtgc acaccccgca aacccttccg acaaaagccc aggcatcctt cggatccctt
    33301 ccctttaaaa tctcttccaa caggcgcacc atggtgccac tgctgaaggc gtcttttatt
    33361 tttccgtttt cgatatctgc ttcgatgcgg agatgacctt caatccttgt gatcgggtca
    33421 acgacgattc ttgtactcat acgattatac ttatgattaa cggttaggat tcaaattcct
    33481 tttcacttat ttttccttcc tgcactttac ccgcgatgag cttatgcttg cgcacattcg
    33541 tcgcgatggc atgggcagca atgcccactc cggtcgcggc cagcgccgcc tttccaaggg
    33601 tatcggcatt cgcctcgatg ccaaaccccg caaaggcgga ggctctttca tagaagcggc
    33661 cattgtccca gaagttctct tcactgcaac cgatgcaacc gtgtccggcc tgaatgggga
    33721 aactggtccc gccgttccat ctcatgactc cacatgcatt ataggtagac ggccccttgc
    33781 atcccacctt atagagacaa tagccttttt tggcattctc atcgtcaaag ctttccacaa
    33841 acaaaccggc atcgtaatag gctctccggt aacaactgtc gtggaccctt ttgctgtaaa
    33901 acgcctttgg tctgcccagg cggtccagtt caggaatttt accaaaggtg accaaatgga
    33961 caatgacgcc ggccatgact tcaccgatcg ggggacaacc cggcactttg atcaagggga
    34021 tccctttcag caatttatga atgggggtgg cattggtcgg atttggccgg gcggcctgga
    34081 cacaaccatt gctggcacaa ctgccccagg cgattacggc tttggctccg gcggctgctt
    34141 cttccaggat ctggagggag gttttccctc caatcataca atatacgccg tctgcccccg
    34201 taggaacgga tccttccaca caaaggatgt attccccgcg gtatttttcc atggtatgct
    34261 tcatggcttc ctcagcctga acccccgatg cggccatcag ggtttccgta tagtcgaggg
    34321 aaatcttgtc cagcagaata tcggctacaa taggatgcga agaccggata aaactttcac
    34381 tgcaacaggt acattcctgg aaatgcaacc agatcaccgg gaccctcgat ttggtttcca
    34441 aagcgtgtac cacctgtcca atggccgaat tttcaagtcc cataaaagca gccatcgtgg
    34501 taacaaaagc cataaattcc ctgcgggaat aacctttcct ctccacttcc tcgtagtagg
    34561 tgggagggct tggcaaagtt tcgtgaatca tgtgaacggg attaagtaaa ataatttaaa
    34621 accacagcct aaaataattt ttaatactgc tagattttat gacttttatt tggctgaatg
    34681 ctgacctggg tcatagaacc aggatctcat tttgctaatt ttgattattt aatcaaattg
    34741 ggttatatga aaaaattcct tcttccggtt ttaactctac tcatagggat gcttcctttg
    34801 accggatggt cccaccccgg ccatggagaa tccgatggat ttacgcttat tcattatttc
    34861 cgtgaacccc tgcacgcggt gggtcccctg ggcatactgg tgacgctact catataccgc
    34921 gtccgctgga gcaggaacaa agcccggcag caaagagcct gaacatgcac gaactgtcca
    34981 ttgtcatgtg catcctcgat atcgcagagg aatatgcggg taaaacaaaa taccgggcaa
    35041 tagaagaaat tgaacttgat atcggctgcc tgaccaccat tgaaatgaac gcctttgaat
    35101 ttgcatggaa ggagggtgtg cggaacacgc ggttgcagga tgccgcaaaa aaaataaacc
    35161 ggataaaagg aaaagcgaaa tgcatggatt gcgaaaacac ctttaccatt gaaaatcttt
    35221 ttgatccctg cccctcctgc gggcagcact tgctggatat tctcgaaggc aaggaattgc
    35281 gggtcaaatc attgatgatg tcatgaatta ttttaaaccc ttaaaccaaa attagcgatg
    35341 tgcgcaacct gcggatgcgg tacggtggat tccgtgcacg ggcatgaagg cggccttccc
    35401 cacgatcact cccatgatca ttcgacccat acccacaagg tggtggatgt ggagaaagat
    35461 atattgcagc aaaacaacct gctggctgaa cgaaaccggg gctatttcga agccaaaaac
    35521 attcttgcgc tgaacctggt cagctccccc ggttccggga aaaccgccct cctggaaaag
    35581 accctgaccg atctcaaagg acaactggaa tttgccgtga tcgaagggga ccagcaaacc
    35641 accaatgatg cggaccgcat ccatgccacc ggaaccaaag taatacagat caataccggc
    35701 aagggatgcc acctggatgc ccacatggtc ctccatgccg tccagggcat caaaccgaag
    35761 caaaacagtg tattgtttat tgaaaatgta ggcaacctgg tttgtcctgc cctgtttgac
    35821 ctgggtgaac gggaacgcgt ggtgatcgta tcggtgactg aaggggaaga caagcctttg
    35881 aaatatcccg acatgttcca ttcgtccacc ctttgcatca tcaacaagac ggacctcttg
    35941 ccctacctgc cttttaatat tgaaaaactg atttccaatg ccaaaaaagt aaatccgaaa
    36001 ctggaaatca ttgagctgag ctgcacccag ggcacgggat tgaacctttg gtatgactgg
    36061 cttaaatcga aagtacctgt tccggaaccc gtagaatgaa tattgttttt cacacgcatc
    36121 gtccttcatg caaacctatc ggattcatat cagcggattg gttcaaggcg ttggcttcag
    36181 gccttacgtc agccgactgg caaaagaata caacctgaaa ggctgggtga gcaatacgaa
    36241 taccggggta tacattgaga ttaccacaga gaaggaggaa gcgctaaaat tctaccatac
    36301 cctcctggaa catcctccgg agaatgccgt gatcagtcgc caccacctgg tggaaaccgc
    36361 ttacgcaacc tatgctcgtt ttaccatccg gcccagcacc ggtgacgaca aaccggacct
    36421 gatgccggct cctgacctgg ccctgtgtgc ggactgcagg aaagaattgc tggataaaac
    36481 gaacaaacgg tttcaatacc cctttaccac ctgtctgcaa tgcggcccca ggtattcgat
    36541 aaccgccgga ttgccttacg acagggcaaa caccaccatg cagcccctgc ccatgtgcaa
    36601 ggcctgcgaa gaagagtacc gggacactga aaaccgaaga cattacagcc agaccaattc
    36661 ctgtaaaacc tgcgggatcc cgatgcacct ctacagccct cccggagtgc tcctgaccag
    36721 cgatccggaa aaaattatgg attccgttca gcaagcgctg aggtccgggc ttattctggc
    36781 agttaaaggc atcggcggtt atcttttgat gtgcgatgcc acgcaggaat ccagcatcca
    36841 taccctcagg agccgaaaac accggccttc caaacctttt gcacttttat atccggattg
    36901 tgaaaccgcg gcaggagatg tcgaattgcg tccatttgaa atcaaagccc tagaatcgaa
    36961 ggcagcgccc attgtattat gtaccctgaa ggcgggcccc gaatccggaa tttgccatgc
    37021 cgccatcgcg cccggcctgg ataaaatcgg cttaatgctg cccggcaccc cattactgtt
    37081 tttaatttca aaagcattcg gaaaacccct gatcgccacc agcggaaacc tcagcgggtc
    37141 gccgatcctc tttaaggacg aagatgcctt gcagcattta tttgaggtag cggaccatat
    37201 cgtcacctat gaccgggaga tcctcgtacc ccaggacgac agcgtaatgc agtttaccgc
    37261 ccagggacaa caggtcatcc tgcgcaggag ccggggtctg gctcccaatt ttttccccaa
    37321 tccatttgat cctgcggacg gaccggtttt ggccatgggc ggagaattga aaagcgcctt
    37381 cgccctcctg gaagaccgca atttttacat aagccaatac ctgggtgacc agggaaaact
    37441 ggaatcccag gcgagttata tccaaacgct ggatcattta acccatctgc ttaaagccca
    37501 tcccaggcat atcctcatcg acaaacatcc ggaatatgag gtctcccgca tgggaaaaca
    37561 aagggccgcc gaaaaaaaca taccggtcac ctccattcaa catcataaag cccattttgg
    37621 cgccgtgctg gccgagaatg atctgctgga tacccgggag cctgtattgg gttttatctg
    37681 ggatggcgcc ggatacgggg acgatggcca aatctgggga ggggagatct ttgtttatac
    37741 ggatcatcac atgaccagaa tggcccattt ggattatttt cctcagcttc ttggcgataa
    37801 gatgagcagg gaaccccgtt tgtccgccct gagcctgctc cgcgggctac ccggaaaaca
    37861 tcaccggttg cttccgtttt ttacattgca ggaatggacg tattattctt cgatggtgaa
    37921 aaaggagccc ccgctgcaga ccagcagtgt gggccggttg ctggacggga ttgcggctct
    37981 gctgggactg tgcgcgcata accgttttga aggggaagcc gccatgctcc tggaagccca
    38041 tgccagaaaa tgcaaggatc cgtcaaccgg tatttacccg cttcccctgg ttcattcccg
    38101 cctcgactgg cgggatttga ttgccggcgt cctggaagac ctggaacaaa accggaaaac
    38161 cgaagacatc gcctggaaag tattttattc cctggcccga ggcgcagcag aggtgagcag
    38221 gcaatgtggc ataaagcgga ttgcgttcag cgggggagtt ttccagaatg ttttattgaa
    38281 tgaattgctc caccgcgtct ttgatgaaaa aaacagtctt tattttcacc ggaaaaccag
    38341 ccctaacgac gaatgcattg gtctcggcca actggcctgt taccacatcc tgaaccggca
    38401 acccgataaa acaggcaaat taaacaggag tacgccggtc ctgaccatcc gatctaaccc
    38461 tttaatttta taaaatgtgc cttgcgattc caggtaaaat cctttccatc agccgtcagc
    38521 tggatgagac tttccagttt ggaacggttt cttttggcgg cattcacaaa gaagtgaatc
    38581 tctgtatggt gcccgaagcc aaagtcaatg attatgtact ggtgcatgtc ggggtggcga
    38641 tcagtgtcat cgacgaagca gaggcccggc tcacctttga gtacctgaaa caaatgggag
    38701 aagtgaatga actgaaaacc ggctccgacc ccggggtttc ttttaattaa agaatgataa
    38761 aaatatagca tgaaatattt atccgaatac cgcgaccccg aaatggtgga tgaattcctg
    38821 caggagatcc atcgcgtgac caccaaaccg tggacgctga tggaagtctg cggagggcag
    38881 acccacagcc tggtcaagaa cggtattctt gatatgcttc ccccccggat cactatggtg
    38941 catggtccgg gttgcccggt ttgtgtcacc agtgtccggg tgatcgacga agcggtcttt
    39001 ctcgccaaac aacccaacat catctttacc tccttcgggg atatgttgcg ggtacccggc
    39061 agcgataaaa acctcctgga agcgaaagcc gaaggggctg atgtgcgcat gctctattcc
    39121 cccctggaag cgcttgaaat tgcgaaagaa aacccggaca aagaagtggt attctttgcc
    39181 gttggctttg aaaccacggc gcccgctaac gctttaagcg tgatccaggc cagccggctg
    39241 ggcatccgga attacagcat tctggcttcc catgtcctgg tgccgcccgc tatggaagcc
    39301 atcctgagcg acccggaaaa caaaatcgac ggatttttag ctgcggggca tgtatgtacc
    39361 atcatgggca tggaggaata ttacccgatt gcggaaaaat tcaaaacccc catcgtgatc
    39421 accggttttg agcccgtgga cttgttgcag ggaatactga tgaccgtgcg gcaattggaa
    39481 gacggaaaag caagggtgga aaaccaatac tcccgatacg tccgaagggc cggcaaccct
    39541 atggccatcc agacgataca agaggttttt gcagttagtg ataaaacctg gaggggcatc
    39601 ggttccattc cgcaaagcgg atatgaagtc aacgaagctt ataaatggta caatgcccgg
    39661 ctcaaattca agctggattt accggcagcc cccgaaaacc cggcctgtat cagcggcgca
    39721 atcatgaaag gaataaaaaa acctaaagat tgccccaatt ttggaacggc ctgcacccct
    39781 gaacatcccc tcggagcccc gatggtcagc agtgaaggcg cctgtgccgc ttattaccat
    39841 tattcttcgc ccgtggaatg ggctcaaccc tgattcaccc cggtcctgtc cggaacaaaa
    39901 agcatatcat gacccacacc gtaaaaatga atttaaattg cccgatgccc aaatttgatt
    39961 atgataaaat cacgctggga cacggcagtg gcggattgct gacccaccgg ttattgaaaa
    40021 gcggtgtgtt tgatctgttc aaaaacagca tgatggacca gcaacacgat ggggcctcct
    40081 ttgaaatcca gggccggcag gccttcagca ccgacagtta tgtcgtttcc cccatttttt
    40141 tccctggcgg aaacataggc gatctcgccg tcaacggcac cgtcaatgac ctggccatgt
    40201 gcggagcttc cgccaaatac ctgtccctga gttttatcat tgaagaaggc ctgaccatgg
    40261 aatcgttttg ggaaatcctg gtcagcatca aaaaagctgc ggataaagcg ggtgtgatgg
    40321 tggtcaccgg ggataccaag gtggtggaaa aaggcaaggg cgaccagctt tttatcaata
    40381 cctccgggat cggacccatc cacccccgtg ctcaaattca ccaccaccgg atcgaagcgg
    40441 gggattccat tgtcgtaagt ggaaatattg ccacccatgg cattaccatc atgagcctgc
    40501 gcaagggact cggtttcgag accgatgtgc ggagcgatac caccaatctc aatcatacgg
    40561 tcattcacct gatcgataag ttcggggaag acatcaaatt cgtccgcgac cccacccggg
    40621 gcggccttgc ttcggttttg aatgaaattg ccgaactcac ccagctgtgt tatcaactgg
    40681 accaaaaatc catccccatt gacccccagg tcgaaggggc atgtgaaatg cttggactgg
    40741 accccctgta tgtcgccaat gaaggcgtct tcgtagccgt gataaaaaaa gagatcaccg
    40801 gggaattcct cgatctgctg aaacgggacg aaagaggcag gcaggccgcc ctcatcggcg
    40861 aagtcaatac cagttacccg ggaaaagtga tcctgaagag ccgcatcggg ggaagccggg
    40921 tagtcaatta tctgaccggg gaacaattgc ccaggatttg ttgaagggga tccggtaaac
    40981 gcggtttacc aataaaagga tatatagtcc ttattccgat ctttgttttc tgcgttccgt
    41041 ttaaaccgct ccagacaaga tgtaccaaat ttttagagtc aacaaagttg taagcctgat
    41101 cccggccctg gccctggtct tcctgagcta taccatttca tccggtcaga aaataaaaag
    41161 agcagccctc cgggaggaaa tggatagcat acttcttgcc catgtggatc gtggcgacat
    41221 tccgggtgcg gtggttcaga tcaaaaaagg gaaaaaaagg atccatcgcc aggcctatgg
    41281 ttatgcccga ctttatgatt cccaaaaacg ggtcttgccg gacccggaga aaatgaccgt
    41341 gaatcatatt tttgacctcg cctccctgac caaggtgata ggtaccacga ccgccctcat
    41401 gtggttggtc gatcaccggc agatttccct ggatgatccg gtcgcgaaat acctagaagc
    41461 ttttaatacc cctgagaaaa tggggatccg gatacgccat ttattgtcgc acagcagcgg
    41521 cctttacgaa tggtatccct tgtattaccg ggcatccagc cgggcggaaa gttattccct
    41581 cattgccagc ctgcccctga aatacaaact gggagaggac cgtcattaca gcgacctggg
    41641 atttgtgatc ctcgggcaac tcatagaacg ggtgtccgga atgaccctgg accagtttat
    41701 ggagcaaaaa atatttaaac cactgggaat gagaaaaacc ctttacaatc cgcaacagaa
    41761 aaaacacttc cggaaaatcg cggccacctc ccacggcaat ccctatgaaa aaagaatggt
    41821 atacgattcc acccttggct ttcaggtcaa agggatcgat cccgaagcct ggaacggctg
    41881 gaggacccat accttgatgg gggaggtgaa tgatggcaat gcctggtatg ccaatggcgg
    41941 cgtttcgggg gccgcaggct tattttccac agccagggat gttcaaaagg tgattgacct
    42001 gatcctgaac cctgaccggg tcagaccatt catttccaaa aaaaccatcg attccttttt
    42061 gagcaaagac cagtataaaa acgggttggg ctggatgatg gatacgacct attcctttat
    42121 ccggggcggg cccgaaggca gttttggcca tacgggtttc accggtacca gcatcgcggt
    42181 cattcctgcc tgtaatattt ccattgttct cctgatcaac cggcagcaca ccggcctttc
    42241 ggaccagggg ctctattata atgtggggcc catccgggaa caggttttta aggcggttct
    42301 aaaatactgc cgttgaagcg cttttataaa accgacgttt cggaacaaca attccacaat
    42361 tcagcaattc agtaattcaa ccctctgtat cctctccttt ccaaaaacaa gaaaggccgg
    42421 ctttcaccgg cccttcctta aaagactata cttgcattga aaaacctcgc tgtatcaata
    42481 gcccggattt tgagggtaag gggtccccgt ggtcaccagg ttgatctggc tctgcgggat
    42541 gggcctcagg taatgaaaat ccttgaatcc ggcgatggga ttgtatttgg ccaggcggtc
    42601 cttgagtgtt ttggtgcgca ccaggtcata ccatcgctgg tattccccgt aaaactcgcg
    42661 ggttcgctcg tccatgataa agtctatgtt caggtcggcc gtcgtgattt tcatggcatt
    42721 cgccgccgcc gcattttgct ccgcattccg gctgcttagg aaggccgccc gctgacggat
    42781 cacatttata tgattggccg cgtcatccag ttttccggcc ttaaaagcgg cttcggcagc
    42841 caccatatac gtgtccgcaa aacggaacat gataaagggg cggtccgacg gatcgttggg
    42901 cgtaaggcgg gtgatgtcat cgtatttctt catggccggg tacattgaac cggtatactg
    42961 gctcggggtg aaaaggaccc cgcggaaagc acgcctttcc tcttcggtga cttccctgcc
    43021 gatcatccag atggccgtat caatgcctct tctcaatgga ccttgcaggg tgttgatcgt
    43081 gatggtccgg ttatagatcc acacggtctg gaaggtttta ttgtatcggg tgtcgttgac
    43141 cctttcaccg aaggcgacat tctgggtata agccgaaggg cggaagcgga ggaagggacg
    43201 gccgttgacc acgtcgcggt ccatcatcac attattgttg acgggcaaac cggaaatacc
    43261 cgcgttgaca tttagggcta ccccgctgta attgggcctc caatagaaga gggacccgtt
    43321 ttgtttgggg ttgtttgctc cgaatcccag tccatagccc gattcactgg ccgtggcatc
    43381 ggtattccgg tcgatcaccc agatggtttc cttgccgtat tcattccctt ccttgttggc
    43441 atcgaaaaaa tcctgccaga ggtcaaggcc gtatttggcc ttatccgcaa tcagttgctg
    43501 ggcggtggcc tgagcattgg caaaatcatc gcctgtcgca gcggaggacc atcccttggt
    43561 caggtaaacc tttgccagga catggagggc gacggctttc gcggcacggc ccggtgaagg
    43621 ggagggtttg tcgggcaatt ccacgctggc ttctttcagg tctttgatga tggccgcata
    43681 aacatcggca atgggctgcc ggctggccga ggtcgaaggt tcggtcacaa aattgaggtt
    43741 caggctcacc tcgccgaagg tctgaaccag caggaagtaa taccaggccc tcagaaattt
    43801 ggcctccgcg atgaccgcct gtttggtagc cgccggcata ttggctgccg gacctaactc
    43861 tatcaccccg ttacaattat tgatggcctg ataagaaata tcccagatgg cttgggcggt
    43921 cccctcggaa gtcaggatcc cataggtaaa caggttgctg gaagatccat caccccgcaa
    43981 catttcatcc gttccggcag aggtggaata ggaaaaccct tcggtgcccc agagaaaacg
    44041 catcagggaa taggcggcga ccaccccggc ctggataccc tcaggcgttt tgaaatattc
    44101 gggggaaaga gaagaccttt cctgctcgtc caggatccgg tcgcagccct ggttgaaaaa
    44161 caggcatcca acggaaagaa ggaccaatat ttttttcttg aaagtcatat tcataatttt
    44221 tgaaaatcga aggattaaaa tttaagattg attccggcat tgaacatccg ggtcggcggc
    44281 gtattgatgc cgaccgtgat ggccctgccg ggagcaccgg cggtcccgct gccctggggt
    44341 gccagggcac ccccgtaacc ggttccttcc ggatcgatgc catagccgtc gcggacaaaa
    44401 ggtgaataga ggatcaatgg attctgcgcc gtcacataaa cttttaatga accgattccg
    44461 tatttcgtca gcagtttctc cggaaaatta tatcccaggt tgacgctgcg gagtttgatg
    44521 aaggacccgt cgtggtaacc cagggtggaa taataatcgg cattgttggt caggccgtcc
    44581 ggctgcggaa attcattggt cggattgtcc ggagtccaat aatcgacctt catctgattg
    44641 acccttccct gtccaaagaa ggcatatcct cccgcacctc cggcattgga ttggaaatag
    44701 gtggctacga cctgaccccc catccgcgca aacatcactg cagagagttc gaaattttta
    44761 taggaaatgc ggttggtcag gcccccgatc cagtccggct ggaaagaacc gagcacctgc
    44821 atatcgtttg cgtcgatctt gccgtccttg ttggcgtctt ccagtttgat cctgcccggg
    44881 atggagttga atttttttgc ctcttccgct tccgaggtct gccatacgcc cagttttttg
    44941 aaatcataaa tgacggtcaa gggttgtccc acaaaccagc cgttgccgat atccctcagt
    45001 tttcccggct cttccagtgc cacgatttcc tcccggttga tggagaagtt caaatcggtg
    45061 gaccaacgga agcctccggg agtctggaca ttgatggaac tgagcgaaac ttccacgccg
    45121 tgtcctttgg tttgggcggc attggtcagg aaggaaccgg ccccgttgct gatgggcagg
    45181 cttttcgata ccaggatgtc tttggttttc tgggtatata catcgatgga acccataatc
    45241 ctgtcccgga acaaaccaaa atcaagtccc aggttttgat tggtggtaga ttcccatttc
    45301 aggtttttat tgggcagggc gctgacaaaa tatcccagta cgttggtcgc cccgaaattg
    45361 taattgttcc gggacaggcc ccccagggta ccataaggct gaacggactg gtttgaggtg
    45421 atcccccatc ccaagcggag tttgaggccg gtgatgaatt tggaattttt gagaaatgct
    45481 tcgtttgcga tattccaacc caaggcaacc gccggatagg taaaccactg gttgcctgga
    45541 gagagcaccg aagacccgtc cctcctgagg gtggcggtca gcagatattt gtcatccagg
    45601 acataattta atctccccat ataagatacc aaacctcttt tggaataacc gttgccgttg
    45661 ttgtttacca cgacgctgtt ggccagggtg aagttgtatt cctgcacata gtccgcgggc
    45721 aggctggtag cctggaaggc gctgctgaac ccctggtctt cctggacact gaacaaaccc
    45781 gtgagggtaa gccggtgtct ttcggcaaag gttttttcat aagtcagcag gttttcgatg
    45841 acgtaggacc aggcctcccc gttgtcgata ttggcctggg tctggccgga tggattgatg
    45901 atggtattgg tcccacggta ctggccccgg taatcctggc ggtaatccag gccgatgttg
    45961 gccctgtatt tgaggccttt gaccagatcc acctcgccat aaaggctgtt gaaagtccgg
    46021 attctcctcc tgctgtcctt gatcgcatct ttattattga gggtcagcgg attggctgtg
    46081 gccgtcccgt ccaccgtacc cagaagggga aagaggttga gacttccgtc ggggaggtat
    46141 ggagacaaca gcgggctgat ccgcatgatg ttaaacatgg gattaatgcc ttccccgtga
    46201 ttgttgctca gggtgttcag ggtgtttagg ccgaccttga tcctgttccc gatgcgcgca
    46261 tcaatggttg aacggatgga agccctgttg aaggaaatgc ccgggagatt gccggtttct
    46321 ttataaaaac caccggataa tccatactgc gtggtttccg atccgccggc aacgctcagt
    46381 tcatgattca tgacagaacc gtcctgataa accaggtcat accagtcggt actggtcccg
    46441 ttggcaatcc cagccaattc atccacgctg aattccacga tgcccccacc cgcattataa
    46501 cgggcatctt ccttgaattt tttgaattcg gggccggtca gaagccggta ttgatcggtt
    46561 tgactgacct ggccgatata tccattatag ctgatctgcg cttttcccgg ttttcctctt
    46621 ttggtcgtaa tcaggatcac accgttggaa cccctggaac catagatggc cgtggcggag
    46681 gcatctttca aaacttcaag accggcgatg tcatcgggat taaggtcgtt gatactccct
    46741 ccgtaaggaa tgccgtccac aatgatcagg ggactgtttt gagcttcgtt ctccccgacg
    46801 gtcctgccca ggctgcggtt gccccggatc cggatggacc cggcagtgcc cggcctgacc
    46861 ccgttgcgtg caatgtccac tccggggatc tggccctgga gggccccgat cacgtttgct
    46921 gaggggactt cgcggagagt ctgctccttg atgcttacga tagacccggt gacatccctc
    46981 cttttctgag tcccgtagcc ggtgaccacg atttcttcca gggaagaggc ggaaggctgg
    47041 atctgtatat tgatgctggc ctgtccgttc accgcgactt cattgacctg gtaacccaca
    47101 taggaaacca ccaggatcgc atccggtgcg gcattcaagc tatagtaacc gtttacatcg
    47161 gtagctgtgc cggtggaagt gcctttaacc aaaatggtgg ctccgggaac cccttcccca
    47221 ttttcatcag aaaccgtacc ggtaacggtg atggactggg cgaaaacagg ggcgttgagg
    47281 accatcagca atggtatcaa cagcttgcgc acccgaacca taaataacat tggatctttt
    47341 atcatatcat ttttttttgt cgtgaaaccg attttttcag atacgaatat aatcaggtgc
    47401 cctgaaaaat tgtaatcgtt tacatttttt ttctagtttt tttttagatt tgtagcgctt
    47461 ttgaaatcgg gttagccaat ggaaagggaa aaagaagtga ccatctacga cctggccgaa
    47521 aggctgaacc tatccatagc gaccatcagc cgggccctga aaaatgaccc ggtcgtcaac
    47581 cccaagacca aaaagcggat cctggaactg gcccagaaaa tgggctaccg caccaactat
    47641 ttcgccaaaa acctccgcac ccgcaggacc aataccatcg gggtcatcat tccccggctc
    47701 aacagctatt ttatgtccac cgtgatcgcg ggcatggaat ccgtggccaa caaagccggc
    47761 tacaacctga tcatctccca atcggcggag tctttcgaaa aggagatcaa cagcgcccga
    47821 accatgttta acaaccgggt ggacggattg ctggtgtccc tggcctatga gtcgaacaac
    47881 ctggaccatt tcaaggcctt cctggaaaaa aaaatcccag tgatcttttt tgaccggacg
    47941 gaggaccagc aatcctttac caatatcgtc ctggacaacc ggaaagccgg atacgacgcc
    48001 accgcccacc tgatcagcca gggctgcagg cgcatcgtgc acatcactgc caataccatg
    48061 aggaatgtct acatcgaccg gatgcgggga taccaggagg ccctgcggga cgcccatatt
    48121 ccttttgatg agcagttggt tctgaaaggg aatctgagca tggaattcgg ccacgcggcc
    48181 gcggatcagg tactggccct tccaacgagg ccggacggga tctttgccgc caacgacaac
    48241 tgcgcggtaa gctgcatgct gcggctcaaa caagcaggga tccaaatccc ccgggacatt
    48301 gccgtggtgg gatttaataa tgacccggtt tcggtcgtgg tggaacccaa tttgaccacc
    48361 atcgactacc cgggctatga gatgggagaa ctggcagcca gccacctcat cagccatctc
    48421 agcggttccc aattgcttca aaagaccaag actattttgt tgcatcatga attgctggtg
    48481 cggaaatcat cccagcgaag ctgagggttt tctactttta tcattgtttt aaaaagcgct
    48541 aatactaaat atggagcaag agaaaaaagt cgccctgatc accggaggcg gttcaggaat
    48601 cggattcggc atagcggaaa aattcattga aaaagggatg caaaccctgc tggtagcccg
    48661 ggacgaagaa aagttgaagg ccgcctgtgc ccagctgggc gcgtcctgtc attacaaaac
    48721 ctgtgacctg gcggacttaa aaacaattcc cgccctgatc caaaacatcc tttccgaata
    48781 cgggcggata gatgtcctgg tcaacaacgc gggcatcaat atgaaaaagg acttcacaga
    48841 agtcaccgat gaggattttc aaaaaataat caccgtcaac ctttgttccg tgttttccat
    48901 atccagggaa gtggtcaaag acatgatccg ccttaaaagc gggtccatta tcaatatcag
    48961 ttccatggcc gcccaatacg gactgccaag ggtgatcgct tattcggctt cgaaatcggc
    49021 catcgaaggc atgacccggg ccatggcctc ggaattatcc ccacatggta tccggatcaa
    49081 ttgcatcgcg cccggtttta ttgagactgc catgactgcc aaagccctga acgacgaccc
    49141 gccacgcaaa gccaaggcct tgggccgcac ccccatgggt aaaatgggaa aacccgcgga
    49201 tatcggtgcc gccgccgctt ttttcgcttc cgatgaatcg gcttatatca caggaaccgt
    49261 cctctgtgta gatggaggaa atgctatcgg attttaggtg gaagcgaagg ttgattgcga
    49321 acccactgca aaactctcca aaaaaaaagg cccgcaaccc atttagttgc gagcctaata
    49381 ttataaaggt ttgtggtgac tgagtaagta cttgttctcc tttaaccgtt caccgttcac
    49441 ccttaacctt ttcaggccat cttccatccc tgcctgtacg gccggctgac aaactgattg
    49501 gcttcgtcat aattcgtcac cttcatgttt ttgccatccc agagcagttt cctgcggccg
    49561 ttgaaggaca ggcggttatt ctgacctgga gtacccagtg aataacttcg tatcgcgagg
    49621 ttgcccatga tcacggtctc ggtcagcgga ccggaataat caaaggagga agtcagcgcc
    49681 tgatgttcct tgcttccgaa accggctttg caggcctggg tccaggaaac ctgatggccg
    49741 tattcgggaa gtttttcgtt gggattggag gtcatataat ccttcggcat ttcgatcttc
    49801 tttccgtttt tgaaatagat ttttgggatc aggccgtagg tcccgcaggt catgacccct
    49861 ttggtgccga tcatcatcac gccgttggta ctgccggcat ctcccaggtc ttcgctggcc
    49921 gggatccatt cggggtggaa ggggcggata cccccgtcgt accaggtgag ggtgatgggt
    49981 tttttgtttt tagccgtggc ggggaattta agctggacat gggatgaagg cggacaacct
    50041 tcagggatgt attccggctg ccagtcctgg gtaaatacct ggcccacact gcattccacc
    50101 tcggtcggat aacccaggcc aagcacccgg aagggcggat cgatgaggtg gcaacccata
    50161 tctcccaagg ctccggtccc gaaattccac cagcctctcc atttgaaagg atgaaagagg
    50221 ggatgatagt ccacatattc ggcaggaccg atgaaaaggt cccagtcttc gcggctcatc
    50281 gatgccggca gggccggttt gtccttgggt acggggatgc cctgcggcca taccgggcgg
    50341 ttggtccaga cgtaaacctg gtccacgggg ccgatcaagc ccttgctgaa ccattcgatc
    50401 atctgttgtt ggcccgggtt ggaagccccc tggttcccca tctgggaaac cactttatat
    50461 ttccgcgcgg cctcggtcag catccttgct tcggccaggt tatgggtaag cggtttttga
    50521 acgtaaacat gcttgccgtt ttgcatggca gccatggcgg cgattgcatg ggtatggtcc
    50581 ggggtggaga tggtcacggc atcgatatcg tttttcatct catcaaacat ccggcggaag
    50641 tccttaaata atttggccga tgaaaattta tcgatggagg ccttgcagcg ggcagtgtcc
    50701 acatcgcaga gggcaaccac ctggttggcc ccgttgttcc aggcattagc aatatcggac
    50761 tgacctttcc cgccggcgcc gatggcggcc aggttgagcc ggtcactggg ggcagtataa
    50821 ccccttcctc ccaggacata ccggggcacg ataaaaaagg aagcggcggc aatcccgctc
    50881 ttttttacga aatcacggcg ttcacggttt atttttttat tcattttagt taatttttag
    50941 gtgcccaatt tagggagatt tttaatacca tagtgctaat ccgggataaa tatgaaccat
    51001 tgatcccttt ctccctttct cctccatttg ataacaattt ccaaacaaag ccaacggtat
    51061 tttatatttt cggaaatttg cagaccggta tcccataata atttggccac cattcagtta
    51121 aacatacatg aataaactct ggcagaaaat ccttcgcatc accaatcctt caggcaaagc
    51181 catccccccg ggccggtaca aattggccag gctcttgtgg gggctgatga tgggaggggt
    51241 cctgtttatc atcctgtttt tcattttcct ttccttccag aacctgccca cctttgacga
    51301 actggaaaac ccccagtaca attatgccac cgtggtttat ttcgatgatg agaccgagct
    51361 gggcaggctg ttcaaacaaa accgggtcgg agtcaattac gatgaccttt ctccctacct
    51421 ggtgcaggcc ctgctctcca cagaagacaa ccggttttac agccatgccg gagtggactt
    51481 tgaagcgact gcccgggtca tcggccgcac catgctcctg gccgataaat cttccggcgg
    51541 aggaagcacc attacccaac agctggccaa attgctttat tccaaccggg atttccgggg
    51601 catgagcaat atggaaaaaa aactcgccct ggtgctgatc aaattcaagg aatggatcac
    51661 ggccatcaaa ctggaacgca gttataccaa ggaagagatc atcgccatgt acctcaacca
    51721 ggtggagttt gtttatgatg cctttggcat caaagcagcc gcggaaacct atttcgacac
    51781 cagccaggat tccctgaaaa tcgaacaggc ggccaccctg gtcggcatgc tggttaaccc
    51841 ggcccgttac aatcccaaac gattccccca aaattccctg tatcgcagaa acctggtcct
    51901 ggaccggatg catacctcag gatacctcag ccgggctcag cgggattccc tgaaacaaat
    51961 cccgctggaa acttcggcat tccggcgccg ggacgtttcg gaaggaaatg caccctattt
    52021 tatcctggag ctcagcaaat ccctccagga catgttccgc aactcggatt taaaaaaacc
    52081 ggacggaagc aactacgata tttataagga cggactgaaa atttatacta ccctggatcc
    52141 ggtgatgcag gaactggctg agcaggccgt gtgggcccat atgcccaaga ttcagaaaca
    52201 gctttttacg gtatggaaaa acagcgatcc ctgggtgttt gaagccgatg accggcaaaa
    52261 agaaatccgg aggtataaac tgaacagcct ggtccgggaa agcgaccggt tcctggggat
    52321 ccgggacaaa atcttgggcg tcctggccga cagcatagac cgtcggtttg attttgtcct
    52381 tactgacaac gatattctgc gcatgcttga ggaagaaaat aaagccggga agatcaacga
    52441 ttacatccac cagggcatat tgagcaatga acgggcgtcc aaataccggc aactcatgga
    52501 tgatcccctt tggccggaac tggtcaaagc ctggaaactg gtattggccg aagccgataa
    52561 agaattcaat acccggatcc gcatgaaggt gtttgattac aatgaatctc ataaaaaaga
    52621 tacttccatg acccccatgg acagcctgcg gtacatgcaa atgtttcttc agacgggcac
    52681 catggccatt gagcccggaa cgggcaagat ccggacctgg gtcggaggga tcgatcaccg
    52741 gtatttcaaa tttgaccaca tcacggcgga ccgccaggtc ggatccacct tcaaaccttt
    52801 catttatgcc acggccatag ggttcaaggc gatttccccc tgtcttaaag tcgtggaccg
    52861 tccttatacc atttccccgg gtgaaggcaa tttcaggctg atttctccct ggtcacccaa
    52921 aaactccgaa ggccggtgga ccgaaacccc gctaagtcta taccagggcc tggcttattc
    52981 ggtaaattcg gtttccgtct tcctcatgaa ggaactgggc aatacggaaa ttgtatgcgg
    53041 gctggtcaat gccatgggga tcgacagttc caaaagaaga cctgacggcg aataccgggt
    53101 gccgagacaa ccctccattt gcctgggttc atccgatctt acggtcatgg agatgaccgg
    53161 ggcttattcg gcttttgcca acgacgggaa attcataaaa ccctatttta tcgaccggat
    53221 tgaagataaa aatggaaggg tgatcttcca ggccaaaatt gaagaatcca gggcactgcc
    53281 ctccgatgtc aattacgtaa tggtcgacct cctccgtcgg gcaagcccgg tgagggatgg
    53341 ctccgttaaa tccatacacg ggggcaaaac cgggaccacc aacagttata aagacggctg
    53401 gtacatggga gtgaccccgg gcctggtcgt cggtacctgg gtgggcggcg accttccgtg
    53461 gatccggttc aaaaccctgg cccagggaca aggatcggtg atggccaaac ccattttcgc
    53521 cgaattcctg aaacgtttgg aagccgaccc gcgggccaac tgggacatta cccgtgattt
    53581 ttacaaaccg gaccggatcg gcatcgaaat gaattgcgca gcttacgact ccctccatat
    53641 acaggatcaa aacctgatca aacaatccaa aaaactggaa gatgaatttg atgactttga
    53701 atattgaaat tggccttgtt tttgttgatt gatctcccgg tgattcgtat caggcccttc
    53761 ctctttgttt tctggtttat cccggtcttc cctttcatcg gctgtaccgg tccggaggtc
    53821 ccggtggagc atgtgcacag caggccacct acggcattgg atagttttat gatcgatttt
    53881 tcggattatt tttcacacca agttagcatt tgcaattgtc ccggcgccgc cctggccatc
    53941 gttgaggata ccaccatcag cctggtcagg ggttatggcc ttcgctccgt ggatgccgcg
    54001 gacccggtgg atgaacacac cctgttcagg ttggcgagcg tgtccaaagg attcggcgcc
    54061 gtgctcactg ccaaactggt cgataaggga ctcctgcact ggaacgaccc ggtgaatcaa
    54121 tacgtcccgg attttaaagt caatccggag gaagaaacgc ccaaaatagc catctccagc
    54181 atcctttccc atagccttgg attgccctac cacagttata ccaatttgat agaagccggc
    54241 aaaagtatca ccgagatcat cccgctgttc agggatgtcc ggctcaacct ccttccgacc
    54301 cagaagtacg cctaccagaa cgcgtccttt gccctcattg aaaaagtggc ggaaaaagct
    54361 tcggggggta agccttttac ctcgctgctg cagcaggaac tattcagccc ccttcgactc
    54421 aaagacatga gttttactta tgaggacatg atcaacagcc ccaacaaggc ccttccccat
    54481 cgtgcagatc ataccggaag atatcatccc gtgaagatca cacccaaata ttacaatgcg
    54541 gtgtccgcgg gagggatcaa cgcttccgcc tccgatatgg ccgtatggtt gcacctcctc
    54601 cttggccatt acccggaagt cttgtctcaa aatgccctgg acagcgtctt cgcgccccat
    54661 atcctcacca gcacggaccg ccgccattac aatttctggc cgggagtcaa agaaacttat
    54721 tacggcctgg gctggaggat cctggacctg ggggatcatt accggtatta tcacggcggt
    54781 tatgccaacg aataccgggc ggagatcgcc atcgatccga aatacaagat cgggatctgc
    54841 gggatgttca atgcgccttg cgacgtggcg gattacctga tccccgcttt tttccagcat
    54901 tatgagcagt ggaaacaact tagaaataat taaggaatta agaattaaag gaattaaggc
    54961 aggatgtgca tccattgatt tccttagaat aaattaaatc gcaaacactt gtttatgggt
    55021 aaaaacagga tcaagaggca accaaggatt ccctcgctta aaggccggca attatccctg
    55081 gttcaaacca aaaaagaatg ggaaggaaag tccctcctgg caaaaaaaga ccaaaaaacc
    55141 tacggaaaga ttatcgaaag caacctcttg gcttttaacc aggacttcct gagggcaaaa
    55201 ggagattcgg ataagatcca taaggctaaa aaagaagcaa gaaccaggct catgaaggag
    55261 ctgaagcgaa gcatccctgg tgttgagaac ctattaaaat caaaactgaa tcctcccgct
    55321 gagtttgtcc ggttcaggga actgcagaag aacattctcg ggaataccaa tatagatact
    55381 tccctggaga cggcgccggc agggcttttg gattataggg tgtttgagcc gccgtttgcc
    55441 ctgggcatat ccggtgaagc cactccatgg ttcctggatg actatacaga tggacatttg
    55501 acccgcaaca gcagtaccct ggaaccctcg ggttttatga gcctgaattt tgacgccaaa
    55561 catgattcca ccccggtcat accctttgtt tcctggggct tttattccat gctgctctcc
    55621 gccggcacgg actatacgat ccctcaaacc ggatatttaa atatcaccgc ggtggccagg
    55681 aatttataca accaggtatt gatttctatt ttggataaag ccggcctcag cgtttcggaa
    55741 atgaatatct ctcatgagat cggcttgttg atcgtccgtt caggagaatt ttatttttac
    55801 cctcaggtga tttttaagga cgggctcatc aatcatgggg gactggacca ggtgcatctt
    55861 ttgcccggat tccctaccca tatcccgcat ttcctgaacc tgactacctc cgatgccttt
    55921 ttaaaaggcg aaacggtaca aatccgcttt tacagcagat tgagttattc ctataaaacg
    55981 gtttgtatga caaattatgt cttagcccgg ttgaattgga aactggataa actgtatttg
    56041 aaaatgactt aatccgtttc tttataaaga catagcgcgg tctaaattag gcctgtatcc
    56101 tttatatgac ttgctaaact agacctggaa ggtttattcg gtatacaata tgaaaacctt
    56161 ccaggtcttg ttatgaggta ataagtccgc gcgcttaatt ccttaatcct taattcttaa
    56221 ttttttaatt ccctcaaaac tccacaataa acccgatcgt cggcgtaaaa aacgggtcgt
    56281 cgtttttcac gcgcgtagga atcgcattgc tgccgtcggg tttcagggcc tggccgtccg
    56341 aagtcaggaa agccgtgttg tcttcgtttc tccggaaagt gtattccgga atttcaggat
    56401 tccgcgccac ataccagttg gtcacatcga taaagaggtc cagggtcgtg cgcctgaaat
    56461 tccatttttt atcgatgcgg atgtcggaac tgtggaagga gttcaggcgc tggctgttga
    56521 gcatggaata atccagtacg ccggtgccct gggacagata attcagcctc gaagccgttt
    56581 cgtcaaaagg ggtgtatggg gcccctccct ggtacctgaa cttcaggccc aactcccagt
    56641 ttcttttgaa tttatacccc caggtgaggg aaagcaggtg gcggttgtcc caactggagg
    56701 ggtaaaaaat accgtcggct ccgctgtatt ggctgtggta ccaggtatag gacaagatgc
    56761 cgaagaaccg ttccgtgagt tttttctggg caaaaaactc taagccatag gccctgcctt
    56821 taccgttggt gatgaccgcc tcattgccca ggactgaata atcccggccc aggttggaca
    56881 gggagatccc ggaacggacg gagaccggca cccgggaata ttttttataa aatccttcca
    56941 gggtaaatcg caagtcctcg cccgggataa actccagccc ggttgcgtaa tgggtgcttc
    57001 ccaggtagtc cgcatcccgg ttgaccagcc ggttttggtt gtcggcaaag cctaatatgg
    57061 tatagggggc cagtttaaaa tatttacccg cagaagcatt cagagtccag gcatcggcaa
    57121 gggaataact cagggagatc cttggagaca aggtattgaa agggttcatg gcaccggtag
    57181 taaacgtatt catatccgtc ctcagtcctg cgctgacgcc taagcggttg gaggcaaacc
    57241 gtttgcccgc ctgtacaaag gcgcctattc tggcgaaagg atcgaggggg ctatcaaaat
    57301 tggccgtcac gccgggctga aggatgttgt tgtcggggtc cctgatttcc ttgcgaagga
    57361 gggcggaggt tttatttgaa tattcgacgt attggaccga agcgccatag gccagtttcc
    57421 attgccctaa gtttttgttg atatcgaggc gcaatttgtt ttccgtttcc ctggaattga
    57481 ggtccagggt ctgggtactg ggctccgggt tttggttgtc ttcaaacttt acgatgtcgt
    57541 tgtcaaaagc cgtgcggctg agggccaagt tgaggtatcc attattgagc aggcgtttga
    57601 gtgaaactcc ggcggtgtag ttccactgat tgatgttggc gttggagttg atcacataga
    57661 gtttttccgg agtgctgttc ttaggggcgg caaagcggaa ttcatccagg gccccaaggc
    57721 cgataaaggt gagggtgttt ttagcatcca ggggcaggtt gagtttggcc tgaaaatccc
    57781 agaaattggg gcggatgggc aggtctatgg cctggaagag gaattgaaga taggatcttc
    57841 gggcggagac cagaaaggtg gagttttttt ctcgggatac cggtccttca agggtggcgg
    57901 caaattccgt agcgctcagg cgggtattgc cctgaaactg gtttgggttg ccgtttttct
    57961 gcttaaactg aaagactgaa cttactgcat tgtcgtattt cgcgtcgaag gccgaggagg
    58021 ccagttttac ctcctcgatg aaactcacat tgaggatccc ctggggcccc ccgctgctgc
    58081 cctgggtctg gaaatggttg atcaccggca cctcgatccc atccaggtaa aacacgtttt
    58141 cattaggcgc ccctccccgg atgatgatat cgttccggaa actgcctccc ccggcgcctc
    58201 cccccacccc gggcagggtt tggatgactt tggagatgtc aaaattgccc ccgggattgc
    58261 tgcggatctc ttccgtggtg actttttgga cggagagagg ggtttcgatg gttgccgctt
    58321 tgacggtgcg tgtggtgccg cggaccacaa tctcggacag ggtgctgatc tcttcctcca
    58381 gttctatggt atagttgctc tcattcccgc tggtgatcac gatattatac agggtatagg
    58441 ttttataccc caggctgctg aacaagaccg aataggtccc caccgggata ttggtgatct
    58501 ggaaataacc cgccgaatct gtggacgtgc cgcggctggt gccctccacc tggatgttga
    58561 ccgcctccag ggcctgctga ctgtttttat ccaccaccct gcctgtgatg ccgccggtgt
    58621 tttgggcgac caggggcctg gccaggctga gcatagaaaa gaggattagg accgtggttt
    58681 tcatacgggg gtagaacaac agaaaattgt ttttgtttaa tgatcactcc tgaatgatca
    58741 cttcctatgg tttatccatt caatacgaac tctccggcgc cccaggcacg aaggatagtt
    58801 tcttaaaggg tattgttttg accttttgac cccaaatcca atgtaccggt accttgatgc
    58861 gggctttttg tccatgaatt caattggatg aaagcgcttt tattgcatcg gattgtgaat
    58921 tgacaaagtg gatatgcccg agtttgttgc tctcaaaaat gaattccttc aaccttttac
    58981 ttttatattt tgagaagtca ccgactacgg ccaggcggac ccggtaattt gaaaactttt
    59041 gaaggagttc accggccatt ccgtttttta ggtcaaaaaa tgcagggttg atgtcccttt
    59101 cttttattaa tatccggtca aacccctgaa aatacaggtt acccaataag tccaaaccgt
    59161 cttccgtttt gtttatgagg atctcctccg aagtcagttc agcaatcttt atattgtcaa
    59221 tatggtgggt gatcagtttc atttctcttc ctttggaacg aatggcttcc tttggctaag
    59281 gactactggt cgtttaatga atcgatgcct tgtgctttaa gcttttccag gccttctttc
    59341 atggctttga cctgttcggg cgcatgattt tgccaatgaa ctacttcccc gacaatttta
    59401 aaagggtggg ttgaccggta ggattgggta gggttgccgg gaaatttttt atcggtcaaa
    59461 tccggatcgt cttcgatggg tccggtgggt tcgaccagat atatcctttc ccgtccctcc
    59521 ccggaggcca gttcagcgcc ccaaatggcg gcgtccaggg tcgcggttag aaaaatgtat
    59581 ttagcatttt tcctttgtcc atagttcgag ttgaaaccca cttcaataaa ctcccccgtt
    59641 tttaagtcgg atctggttcc gtggaaatag gtctgggcga aggggcttgc gtttttggta
    59701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    59761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taaaatcatt cttttccgta
    59821 aaatactcca gtcccaaaac caggcccatg gaaccgaaca gagaaacttt ctgcatgatc
    59881 gccaataaag cccaatcgcc cgcaccgtat aagtataaag tataataaaa agtcaaaaga
    59941 ccaaggaggc agcagacctt aatgaaatga agtcttgtcc tcagcaggta aaccgtgatg
    60001 taaaacaagc ccatcaggaa cgaggtgctg gtgatgatga tcatgaggtc gtgcagtcgg
    60061 gtggtgatga gtatgtttaa aaatatattc gtataaccga tcatttttaa gaccctcgcg
    60121 gcatgatttg ctgaaatttt tgaagccata ttgataaaaa acaatccata acccaaagca
    60181 tgaaatgcca tacccgggat tgcccaaaat ctggacggat tgtccacgcc attcattgct
    60241 ttgaccgaaa acaggttgct gataaaattc ttggtccagt caaaccccgt ggagttcttg
    60301 tcgagcaaag agccgcctgg ataaacccag gtggccgcca gcatcaaaat caaggaaagg
    60361 atcacacata tcaaaacgga gtatctcttg atcatttgcc tctggttatt taaaattagt
    60421 attaaccggt gagtgcctaa aggagggtca tcgctgcacc tttttctcag ccgccccctt
    60481 gagttcctgc agatctttaa tgataccgga gtccagattt ttctgaaaat aacctttaaa
    60541 taatttgggg aatactccct ggaggctctc ttccactctg accaccgtgg tatttccttt
    60601 taattcaaaa aaccaattgt gaatcgcgct tgccccgaag gtggtcccgg tccagccaaa
    60661 gcctgatttt ggattggagg tatggatcct ggattggaag gaaaggccgc cggctttcca
    60721 gtcaaattcc gttccctgtt ttatctcccc gtgaaccact gcttttttaa tatccttttt
    60781 ccattccggc catttaggga tgtcggtcag cgtttcccaa accacatcca aggaagcttc
    60841 gatttcaatt tgattttggg atctaaccgg tgccttttca ttgattggaa tattcatatt
    60901 gtattgaacg atctgatttt caaggtattt attgaacgaa acacgctcat aataaaaaaa
    60961 tgacaatata ctcaatatca ccaaagttaa aatagagaat cccttaaacc tcatattggc
    61021 agcagcaata ctcaatggtc ttttaaattt tgttcgtcag atacaaccgt ctgtttttgt
    61081 ttacctcctt cactccctga acggataccg tctgcttcac tttcgtcatt ttccttttta
    61141 accttaattc ttaattcaac tacgtatacc ctagaattat tcccaatatc actacaatgc
    61201 ccagcacgcc ttccagtcca tgaataatca ccgccatcca ggatgattga aaaagttttg
    61261 acggtaaagc aaattcaaac ccgaaataaa gcgcggtgga caatatgatc tgcggcttat
    61321 gaaggtgata gaacccaaat aacaccccgt taaaaaacca gtcccagttg ccaaaaactc
    61381 ctttcatttt aggcaatagg atgcctctgt aaaaaaattc ttcacccaat aagtagttga
    61441 atatcgcggt gatcagatag agccccagga tccaccaggc gccccggtat tcggccgcag
    61501 ctaaactgga taaatcatat tttggcaaat cctggatcaa tggagccatt aagccatcca
    61561 cgtttggaag gccagaaata ccagattgca gaaccgcact taataagata aaagggattg
    61621 cccataaaaa gagccaatag cttgacttgc cggttttggg attcactggt tttcgatact
    61681 gcgttctttt tgcaaccgtc tgccagttca atgcatgccc ttcctgctta agaattaata
    61741 aggagaggat gaattgccaa accagtccaa ggagtacagc cacccagtag acgattaacc
    61801 tgggccaatt tgtaaccgaa accacgcctg gtgtaatgac aaatgccaga atgcccatgg
    61861 gcaatgccga taaagcccat aatattatga tctttccaat gctatactga tcccccgctt
    61921 tacccaaatg ctttgaattc acggcttaaa cacttcatta ttaattatta attattaatt
    61981 ttcttagtcc tgcccacagc ccaattatga cttaggtgta ttcttcagca ttaacggttt
    62041 ttttattaat cagcatccaa accatcaatg ccatatacca aatggctaca aaaggtccaa
    62101 aatcaacgga tccggcttcg cctgggggct ccgggaaata agccaggttg aagccaagca
    62161 aaagaatggc aaaaaccatt cccaggacac ttagtatttt atgaatttta gggtgtttat
    62221 acattactaa agcgaaaaag aaagtcccga ttgaaataaa tatatcccag gccaaatcca
    62281 ttcccaactg aactgagttt acttcttgat atatccattt cagttgttct tctgaaacac
    62341 tgccccggtc agccgcttta aactgctggt gataggcctg gatggtaagc tggacggtaa
    62401 gcatcatcgt aagcatggcg gtggctacaa tattgaaaag caccgcaata cgcaagttga
    62461 tggaatccct ccattgccgc aatataaagt aaagtcccac tgaggccaac atgaacaaag
    62521 ggccaaaagc aaaggcaagc acaacagata ttttttcatg gaaaggggca aaggccgcaa
    62581 gaaaatacga aaggaatgcg gagagcccgc aataaatggt caatgagagc cactttttac
    62641 ttagaatggt ttgcatatga cttaagataa aagggtattg ttttgatggt aagcgggctc
    62701 gatattattt tcgatttaaa aataaggcaa acgtttacct tctcatgatg attttatcta
    62761 aaactctcct ttcgccaaac agtcggccca tcttgctttc ccatgaaatg ggtaagcttc
    62821 ttcattttta attcctttca ttctttaatt ccctattaat gattccaccc accccccacc
    62881 agatcaaggg aggcaaaccc atgctcaaac ccacagccca atacggatgt tcataccccg
    62941 aagcccccac ccaggcatag accgcggcca tcgggagggt cccggtcagc aaggccagat
    63001 ggaataaagg ggcattcatc cgggtcaggc cggccatacc actgactact tccgggaaaa
    63061 cgggcaacca gcgggacaag accacgatcc agctgcccaa cttagacgag atcttctggc
    63121 cccgttcaaa atccttcctg cccaggagga ccagggcggt cttttggccc atcatccggc
    63181 aaagccagta tcccagcgaa ccggctaaaa aagaacccgc agttccaatc agcatgccca
    63241 gccagggccc gtataaaaac cccagggcag ataatatgat ggtggcgggc agcggcagga
    63301 ccagatcggc catcagcagc ataatggcac ccagccaggc ccagggccca aaacggtcca
    63361 gccattgcag ggtgccttcc tggctaaaag ccagcatcag gctgtccccc cacaaggcaa
    63421 aagccaggag gaccagggcg gcgaaaccta tgaaaaccag gagcaatcgc atggagtaaa
    63481 gataagagaa ggggcgactg caagtttccc accaaggcca aatgttcaaa gctggccctc
    63541 tgttggggat aagtttggct atgtttccca cgacggccac caagcacaca aaaacatact
    63601 ttgatattgt gttattattt tcatagccct catagtgtcg ttgtgtcgtt gtgggaaccc
    63661 tttgttgggg aggagcttag ctccgacccc ctgtttccca ttcactcccc atgttcatat
    63721 tcatggccaa agtcaaactt tgtccttagt tcccacgacg gccaccaagg acacaaaaac
    63781 atactttgtt attgtggtat tattttcgta gcccgctgca tagtgtcgtt gtgtcgttgt
    63841 gggaaccctt tgttgcgatg gagcgttggg gaggagctta gctccgaccc cctgtttcct
    63901 attcaatact tatgtaaata tacctggcca aacccccata tttactacac ggccaaagct
    63961 aagctttgtc ctaacatcta tacctggcca ggtcaagttt tgtcctcagt ttcccacgac
    64021 gggcacaaag gacgcaaaaa caaacttgct catgcgttgt gtcgttgtgg gaaccctttg
    64081 ttggggagga gcttagctcc gaccccctgc ttcccattac ctcctcgtcc taaccattaa
    64141 tgggcacctc ataatattcc agcgcccatt ccaccatata cctgacccgc tctggcagta
    64201 tcttatatac gatgagttcc ggattgtcga tggatccgag ataatgctgc agcaaccggt
    64261 tggctgacca gatctcttcc agcagggcgc ggtcccggac catttcggcc cggccggtga
    64321 tccggacctg gttgtggtcc tcgtccatat aacacaattc cacctttgga ttggaggcga
    64381 tctccgccgt ctttccgtag gacctgagat tggctatata aaccacgaat ccttcggtgc
    64441 ggaccgggga tacgggcctg acccgcggct gatctccatc cagggtggcg agcgtgggga
    64501 agcgggcctt ctttacgagg tcccgggcca atgccgggat ctccgatggc tggacggggt
    64561 tgggtttggg tttaggcata aacaaaagta aatttaggac ttatttcaag ggttcatttg
    64621 cactccgcaa ataacagggt ctaaaaaaac atttctttgg aaaaagatta caaacaggtc
    64681 ctcctcagga ttctcaatac ttatgcaccg gtggacaaaa ccgcggcgga tttgatgttc
    64741 gcaagcggga gcatggagaa agtaaaaaag aatgaaatcg tcttccagga aaataggttt
    64801 gaggccttcg agtactttca gttggaaggc gtttcgcacc ggtacaacct ggacgaggat
    64861 atgcaggccc tgaccaccgg gatctatcag ggcgaaaccg tgatcacccc tcattttgcg
    64921 cggaccacca acggccagag catattcacc ctgcaggcat tgatggacag tgtctacctg
    64981 aaaatcccgg ccggcctttt cagggaaatc agcgatcaaa accagcagat ccggatgttt
    65041 ggaagggccg tgattgaaaa agaattcatc cgcagcctca attttgaaat cctcttccgt
    65101 gccttcaatg ccaaagaccg cctgatctgg ttccggacca aatattcatc actggaaaac
    65161 ctcatccccc acaccatcat cgcctccttc ctggggatta cccctgtttc cttcagcagg
    65221 ttgcggaatg atttggccag gaaataggga tctgtaattc ttatcatttg ataagccgct
    65281 tcttatcaat tgataaggtc cgggagccgg ggggcccttt cctttgtacc cggaatattt
    65341 attcaggctt ttatggtatc cttcctccgc aaaatgaaac gcatcctgat cctgatttct
    65401 ctcctcacac tgggcttgct gatcgtcatc cggcccgcca ataaatcata ccgcatgaaa
    65461 aatatagacc tatttctaaa agaccaggtg gaccagggcc ggaccccttc catccaatat
    65521 gccttttttg acaccggaag ggtgattttt gaatcgcatt acggatggag ggacctgata
    65581 tcccggaaag ccgtggacgc ttccaccacc taccacctgt tttccattac caaaaccttt
    65641 acagccctgg ccgtccttca actggcccag tcgggaaaga tcggattgga caagccggtt
    65701 tccacctacc tgcccgtgtt tccttacccg gaggacatca ccatcgaaca attgctgagt
    65761 cataccgccg ggatccccaa tcccctcccc ctcaaatgga ttcacctggc cgaagagcac
    65821 ccttctttca ggcgggaccc cttttttgcc gaagtgttta aagcccatcc caaaacggat
    65881 tttgagccgg gtacaaaatt caaatacagc aacctgggtt atgtccttct cggccaattg
    65941 gtcgaggcgg tttcgggtca gccttttgaa gaatacgtaa atcaaaatat cctggcacgc
    66001 tgcggtcccg gcctgagctt tgaactggat ccctcaagcc atgcggtggg ctatcacaaa
    66061 tggtggacct tcacgaatgc gattttcggc ttcctcattg acaggtcaaa atatatggga
    66121 aggaaagaag gaaagtgggt ccccttcaaa cccttttaca acaacggtac ggcctatgga
    66181 gggctgtttg gctctgccag cggcctgatc cggtacgccc aaaacctcct ggacccccat
    66241 tcggtgctgt taaataatga ttacaaagaa atcctgttct cggaaaaagt cgtcaggaat
    66301 aaaactaccg gcatgtccct ttcctggttc accggaaaac tgaaagggca cgaatacctt
    66361 gcccatgccg gcggtggagg cggatattat tgcgaactca ggatttatcc ccggctggga
    66421 gtgggcagcg tcatcctgta caatcgctcg gggatgaggg atgaaaggat tcttgaccgg
    66481 gcagacgctt tttttattac tgaaaagccc aggcaggaat gagcgttaat tagttaatca
    66541 gttgccagtt ggtcagttaa ttccttccgt ctccctcctg gtgattcgct ttggggactg
    66601 caatttatgg tcccttcctc ctgtctcctg tctactttgt ccgtttcgct cccttcgccc
    66661 ttaaccttta accctttccc ttaaaattcc tattttgtcc agggaatata agtatatacc
    66721 ggacctccca aaaaaatgaa acaaaaactc aaaaccaccc tcgctttcac caaaaatctc
    66781 tttaccaccg gagcctttat ggaaaccagc gagaaggtgg aaaaagaaat ttgcaaacac
    66841 ctctccccgg aagaaaacac cctcgtggtg gaatatggta tggggcacgg caacatcacc
    66901 caaaacatcc tgaaacacat ttcaaaaacc tcaagggtgt atgcttttga agtgaatccg
    66961 gatttctgcg actttgtgcg caaggccata cccgatgacc gcctgaccat tatccaggac
    67021 agtgccgaaa acgtgaagaa tcacgtaaaa caggaggtgg acagcgtcat ttcatccatc
    67081 ccctttacat tcctgtccaa acatcagtca gaaaaaatac ttgcagattc ctatgaattg
    67141 atgcgcaagg gttcctactt cagccaggtt ctttatacca aattcaattt taaaaaattt
    67201 gtacccgttt ttgaccaatg tgaaatcatc cgtttaaagc actttcccac ggagtatatc
    67261 taccactgcc tgaagaacgg aaaacgaaag gaattggcct gacaaaaggg gacatgaatc
    67321 gaagccaaaa ccaggttgcg ccgcttggcg gatcatttgt acccattgag gccaccggct
    67381 atttcatttc cagataaaat cccctaattt tacccaaacc ggtttttaac ttttgcacat
    67441 ggatcttagg ccaggcgtga tattattatg tctattgtgt tttgccggca gcttgactgc
    67501 cgaagactct gtgcgttatg agaccgatgt gcgtcctctt cttcaaaaaa gatgcatcag
    67561 ttgtcacaat accaatgccc ccaagggcgg ggtaaatatc gataactaca aggagcagga
    67621 ccgggtgatc aaagacgggc aattctggct taaggtcctc gaccagatca aaacgggggc
    67681 catgcccccc aaaagcgagc ccaaactttc cgaagcggac taccacggac tggtggatgg
    67741 gattaacagc atcttgcaga cttcgcttcg ccaacggtcc gccggtcatg tggtgatccg
    67801 caggctcagc cattccgaat accgttacac catcctggac ctgatgcagg tggattttga
    67861 tgccaggaat tattttcctt ccgatggttc cggcgggggc ggctttgaca accagggcag
    67921 ggccttgttt ttcacccccc tgaaactcga gcggtattac gatgcggccg accacatcgt
    67981 ggaacaggcc tataaggacc ccaaaatctg gggcgatctg gtcccggtcg agttccggca
    68041 gaacgtattt caacggttgg gaaactggtt cctgtccctg tttaccgata gctaccgtca
    68101 gttcaatgcc ccggaaaacg cggccgaaaa ggtgatttat ccatttgcca ccagggccta
    68161 ccggcgcttt ttgaaagacg aagaaaaaac gaggctgatc gatttgttca gctcggttta
    68221 ccatcggaaa gattccgtaa aaaacccgca gcgtttcaac gaaagcattg cccaggtcta
    68281 taaggcggtc ctggtttccc ccaatttttt ataccggacc gaagaagaac cggagaaaat
    68341 gggctcctat ccgctcaccc aatttgaggt ggccacccgc atgtcttatt tcctttggtc
    68401 ggccctgccg gaccaggagc tttttgaact ggcccgggca ggcaggctgg atgataccct
    68461 cgtcctggaa gcccaagtca agcgcatgct cgctgatccc aaatcccgcc gttttgcgga
    68521 gaatttttca agtcaatggc tgggcattac caaactgatc gatccccagc ccatagccga
    68581 tccggaattg tttcccgatt ttaacatgcg catccgcaag gcgatgtacg atgaaagcgt
    68641 ggagtacttt tatcacgtcc tcacccaaag caaaaacttc ctcgacctca tccaaagcaa
    68701 ctatactttt ctcaataaag acctggcgga atattacggt ataccgggca tcaccgggga
    68761 ccaattggtt aaaacgactt tgcctgattc cactcgcggc ggggtgctgg gcatgggcag
    68821 cgtgctgacg acgacctctt ttgccacccg gaccagcccc gtaaaccggg gcaaatgggt
    68881 gatggaacag atcatgggca tttcccctcc ccctccccct gaggtcgtgg ccgaactgac
    68941 cgaagacaag gccattcacg acaaactggg gctgcggaag atcctcgaaa ggcaccgggc
    69001 agaccccaat tgcaactcct gccatgtgaa aatggacccc cttggcctcg ggctcgaaaa
    69061 ttttgacccg atcggccgat ggagggatag ttatggtatg gtcccggtcg atgcttcggg
    69121 agtcctctcg gatggaagac cctttaaagg gccgggggaa cttaaatccc tgctgttgac
    69181 cgaaaaagcc aagtttgccc gtaacctttc cacaaaaatg ttatcttttg ccctcggcag
    69241 gtctatcctc tttacggatg agcccgccct tcaggttcta gagtccaccc ttctggccac
    69301 tgatttcaac cccgaacctt ttatcgtcga actggtgaaa agttacccgt tccggatgaa
    69361 attaaatgac ttcgaaaaaa agatctgaac cgatgaataa gaaatggcat atcacacgca
    69421 gaaggatgct caagggcctc ggggccagca tagctcttcc ctatcttgaa gcgatggccc
    69481 tgcccgcatg gaaaccttcc ggcgtggtga aggctcctat ccgctccgcc ttcctcttta
    69541 tgcccaatgg agtgcatcct gaccgctgga cgccttcggc gaccggccgc gattttgcgc
    69601 tgacccagca actccagccc cttgcaaatt tgaaaaagga catcctggtc ctgggggaac
    69661 tgatgaataa aaattccatc ttcaaagggg cggatggcca ctatgccaaa tcggccagcc
    69721 tgctgacctg catgccgatc aaacaaacgg tgggggacaa catcagcagc gggggcgttt
    69781 ccgtcgacca gctcatcgcc tccaaaatcg gacatgaaac ccttttcccc tccctggaat
    69841 atggtttgga ccggatcacc accggggtgg acatcaatgt gggatttacc cgcctttacg
    69901 gatccagcat ctcatgggcc acaccggcac aacccatgtc taaggaaatc gatccccgcc
    69961 ttgcttttga ccgtctgttc cggaccttcg tacccggtaa aaaaataaag gaggatccct
    70021 ggaaacaaag catcctggat gcggtcatgg aggatgccac cagcctccgg aaaaaccttg
    70081 gacgggccga ccaggacaaa ctcaccgaat acctggaatc catccgcagc gtggaaaaac
    70141 ggatctccaa cggaaccaac ctggaaaaat tcaaagccaa tatcaccccg gatatccgga
    70201 aagaactggt tcgcgtcaat acccggatcg atgagtatgt ggaagtttac ggcggcgtgg
    70261 acatcacgga gaagacccgc ctcatgctgg acatcatggc cctggctttc tggagcgatg
    70321 ccagtcgggt ctccacattt atgtttggca attccgtcag caaccgcaac ttctccttcc
    70381 tggaaggggt gaaaggaaat caccattcca tttcccacca tatggacaag gccagtttcc
    70441 tcgaagaata tacccgcatc accgcctggc atgtggaaca atatgcctac ctcctcgaga
    70501 aactgaaaag cctgaaagaa ggagacagca atgtcctgga aaactccatg attgtcttcg
    70561 cctcggatct tcgtgacggc aaccggcatt ctccgcgcaa cctgcctatt ttgctgggtg
    70621 gacaaggggg aaggaaaatc aaaaccggcc aaaacctgaa ttatgccaag gaaacccctc
    70681 tggccaatct ttatctgacc atgctccaag ccatggatat tgaagaagaa agattcggag
    70741 acagtaccgg cacccttgca ggaattgcct gacgggcggc agaaaaatcc attgaaagga
    70801 tccgatgatg catgcgaaac ccgggatacc gcggccgctc ctggcggttt tattgggcct
    70861 gatcggcctg ggattcctgt ttttgcccgg gcttgcgccg ccggctgaaa tacctgtaat
    70921 tgctctattg ctgggcaggg cccaccccct ggttttgcat tttccaattg tgctgatcat
    70981 gctggccctg gtttttgaat tgttctgccg ctttaaggtg gtggaaagga gtgaaacgtt
    71041 tatccgcgtc atcttgattt tgtccttgtt gtcttttgtc gccgcgatcg caagcggcta
    71101 ttttctcttt gcttccggag aatattcagg caggctgatg acctcgcatt tttggggggc
    71161 agcgatctcg ggggcagctg cctttctcac tgcgggcatc tacttttttc ttccgaaaaa
    71221 ctccgggctt ttttccctgt acctcagcgg tttgtttgtc agcaatgtag ccattgtgta
    71281 tacggggcac ctggggggct ccctcaccca cggccgtgat ttcctcagcg aatacacccc
    71341 cctgcttttt caaaaaccgg aaagttccgg cccaaaggat gcttccgaaa tgctggttta
    71401 caccgacctg atcgctccgg tccttgaagc taaatgtatc agttgccaca acagccagcg
    71461 ggcaaaaggg ggcctggcca tggatagtta tgcctccctg ttttcacccg gcgaaagcgg
    71521 ccagccctcg gtcacggccg gagaccccga aaaaagtgaa tccctaaaaa ggatctacct
    71581 gccggcggac catcccgacc atatgccccc ggaaggcaaa agccccctct cccaggatga
    71641 gatccgcctg ctccggtact gggttcagac cggggcatcg gatagtctgc ggttgaccga
    71701 agcttcgaag acggattcca ttgccctcgc gatagaaaac atgaggccgg tattggcgcg
    71761 ttatcagcac aaagcggaag cggccagcct gaatcaaaaa atcatccgcg cggaactgga
    71821 atcccttgct gaaaaacttc aggtaatcat tgaaccggat agcctgggag aggccaatga
    71881 atattccctg tccatgaaat ttcctcccgc tccttttacc aatgaccaga ttcagtcatt
    71941 aaaaccttat ttcccttact tcagcaaact gtccctggta tccagcggca ttgacgatga
    72001 cggactctat tatctcggga agatggaaaa tctccgtgaa ttgtacctgc ttaaaacggg
    72061 cctggacggt tccggcctgg tccacctcca gcacctgaaa aaactggaga tcctcaatct
    72121 ttcttatacc aaaaccgacg atcaatcggc cttcgacctg atgaaaatcc cgaatttaaa
    72181 aaccgtgtac ctgtaccgga ccaatgtttc ccggcaagtg atggaggcga tcatgaaata
    72241 taagcgtgga ttgatcctgc tggaagaaga aggggtgttt tgaacccacc gctacgcgtt
    72301 tctttctgaa acgccgtctt tcttcaattt tctgcaggca cttcataatc ttgtttaact
    72361 tcatcccatg tcaccttccc tatttccccg catgcacgtc agcctctacg tcagcgacct
    72421 ggaccaaacc atcgcatttt ataactcgtt ctttgggcag tcgccggtca aggtcaagac
    72481 tcattacgcc aaatacgtgc tggagcagcc ttccttgatc atttctttcg tggaacataa
    72541 agaccgggta caatcccatt tcgggcacct cggtttccag gtcgaaacgc cggaggtcat
    72601 gcatgcggcc ctggacagaa tgaaagaatc aggccttgaa acccgggagg aaatgggggt
    72661 caattgctgt tttgcaaagc aggacaaatt ctgggtcatg gatcccgatg gagtggaatg
    72721 ggaggtttat tattttcatg aagatgcgga attcaacgat ccgcgttacg aaatggagga
    72781 tcaaaaagtg cagaaaacag ccatgactga aatattcagc acaccggtca aagagccctg
    72841 ttgcgcaccc ggggaaggtt catccggagg gaaaccctgt tgttaatctg aacaacaacc
    72901 ttcctgtcta tcccacaaat cctgttaatc ctattaatcc taccaatcct attaatccta
    72961 ttaatcctat taatcctata tggatataca tttttttaaa aaccagcagg aattcagaga
    73021 ctggctggat aaaaatcatg gcgaagcacg ggaaatccac gtgggattct acaaaaaaga
    73081 aaccgggaaa ccctctctga cctggccgga atcggtggac caggcgctct gttatggatg
    73141 gatcgacggc atacgccggt ccctggggcc ggaatcctac accatccggt ttaccccacg
    73201 caaacccagg agcatctgga gcgccgtcaa cctggctaag atggaaatcc tgattcgcga
    73261 aaagaaagtc aggcctgccg gtatggccat ttatgaaaag cgcgatctgg ataaaataaa
    73321 gaaatattcc ttcgagcagg agaaagatcc caccttaagc ccggccctgc tccggtcatt
    73381 caaatccaat aagaaggcct atgcctggtt taaagcctcg gctccttctt atcaaaaagc
    73441 ggccatccac tgggtgatga gcgccagacg ggaggccacc caaaacacca gattaaatac
    73501 cctgattgaa gatagcgcgc agggaaggag gattgggctg atgaggcgct gaggttgaga
    73561 ttgaagttga ggttaaggtt aaggttgagg ttaagtaggt cctcctcagc gggtgtattc
    73621 aataaagtgt gtcaggtact tcagggcagg ctgctgagtc tgag
//
LOCUS       DAOQXT010000010        72062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_375_length_72062_cov_7.576902, whole genome shotgun sequence.
ACCESSION   DAOQXT010000010 DAOQXT010000000
VERSION     DAOQXT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 72062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72062
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_72062_cov_7.576902"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..503)
                     /locus_tag="PKM27_02735"
     CDS             complement(210..503)
                     /locus_tag="PKM27_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06203.1"
                     /translation="MEEAQYQKKSLQFTNLKSYTTTVFLFMYVIYGANKHIIKLDIIY
                     LGILFAAIINLGLSFYMLRLNSALRDINYSKNTWRIMAAIVFNGLMIFLAYYS"
     gene            complement(580..2451)
                     /locus_tag="PKM27_02740"
     CDS             complement(580..2451)
                     /locus_tag="PKM27_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNR06204.1"
                     /translation="MNRLKAIARGEENLLKEMKELLNSGQNLPTATRGNIDSKLVRAV
                     IQDFDKADHLPQVEAVVVPYMRPVLFVKNGKIELPESNELKDRMIKYRPIVERPLKSV
                     GRIELKNHQFKNIGTGWLVSDEVVVTNRHVAEVFAVKSKPTALGASFKPNFIGDPIQA
                     VIDFKEEYALNTPAKNQFEIRIEKVLFLADSIKGAPDIALLKIKKSNRLPDPIPFISN
                     KLRVNQFIGVVGYPFQDPRGVTDPEMERRIFGNVFGVKRYAPGQIIGNPRSKWYFLHD
                     ASTLGGNSGSLVQDMETGGAIGLHFGGLLLEANYAVKGAEILETLAKLNIKNFHAFSD
                     QGSKVLVEHGFVQEKAFPVKEYSDRKGYELNFLDGNRVSLPTVKTRSDILKFGKNKKE
                     SVIKYTHFSVLMSKSRRLCFYSAVNIDGKQYVRLKRPGWRYDSRIPKGAQIMNECYGD
                     APRFSRGHMTRREDPNWGPLASAGNEDSMHVTNVVPQMQPFNGGVWLTLENYALENSK
                     QDKQKISVFTGPFFHDNDPVRDGVRIPVAFWKVIAFIHDDTGKLCATGYTISQEDYLN
                     MEEFIFGEFQTYQVPIRWIEKKAGISFGKLRTIDPLAADVFESIPSPLQSLRQIRFY"
     gene            2875..3255
                     /locus_tag="PKM27_02745"
     CDS             2875..3255
                     /locus_tag="PKM27_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06205.1"
                     /translation="MTYSYLERTTDAEQIKMRTESFSSHSDEALRHIVQEARETGGPH
                     SHDQALDLIALHPILIRRFGKSPVQMEGRKFIRLDPEPLPDPAKEEKLRQFRSKHQQM
                     LSALARKGYDQLNDEERAYWNSVY"
     gene            complement(3289..3801)
                     /locus_tag="PKM27_02750"
     CDS             complement(3289..3801)
                     /locus_tag="PKM27_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNR06206.1"
                     /translation="MQYDWTTFTKRIPVHAPLADVYTAWATRDGIESWFLRTSLFTRA
                     DGEERGPHDYIQLGDNYTWRWHGWGDEVTHYGNILDANGQDRLAFTFDAGNKSNDMQV
                     RVDLSREKGQTIVELTQYHIPTDEESKFMYHLGCMEGWTFYLANLKSILEGGIDLRNK
                     DVEIKKVINS"
     gene            complement(3931..4365)
                     /locus_tag="PKM27_02755"
     CDS             complement(3931..4365)
                     /locus_tag="PKM27_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HNR06207.1"
                     /translation="MDDNQAPGLKKIYDEKGNALSPILPEGVKNFLIDIDGTICDDVP
                     NEEPERMRHVAPYPDALEIINKWYDQGHIIYYFTSRTEEHRQITEEWLRKHGFKYHGM
                     LMGKPRGGNYHWIDNHIVRATRFKGKFTELIIETKEIEVFKD"
     gene            complement(4407..5231)
                     /locus_tag="PKM27_02760"
     CDS             complement(4407..5231)
                     /locus_tag="PKM27_02760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNR06208.1"
                     /translation="MVILNHETYKEVGSYQLNLLNVYVLLSMLMVVGALLVTLLLFFT
                     PMKRLIPGFASESSGDYIELYKKVERMETEIGAQQLYISKMQKLINGEVEIPNPDQYK
                     SEIKALPANQTPVPKSEEEMALEDEQLLENQIANVKNTNLSNVSRSLPIEQLFFIAPV
                     VGEVSEKMSSQKSHLGIDIIVPKNTEVKATMDGNIIAADWTQETGNSLVIQHPNEIIS
                     VYRHNNKLLKKAGDYVKAGEAVAIAGNSGDLTSGPHLHFELWYRGKAVNPEDHIKF"
     gene            5366..8347
                     /locus_tag="PKM27_02765"
     CDS             5366..8347
                     /locus_tag="PKM27_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06209.1"
                     /translation="MKLSPISRLSLVALAFLANACAVQKSKKDISGFGLFYHNLTAKY
                     NGYFNARELVDLSQETLTNQYRDNFSKILELYEYNGVDNPAAATANLDIAIKKASTVI
                     NLHRPSHWVGDSYLLIGQAQYLKQDYETAEETFKYMVQNYNKLNEGIEKKKSGSRSAK
                     TKEQIKAQEEAKKEREEQKKDLEKERKKKIKTREQENKEKAKARAQMIKDRKKGIKTT
                     PPAKSAVNAPKPKSSADSTGAAGTSASAVKKTSALKKAKEETEEKTGTPRPPKGKHRP
                     VLQDGQLWLAKTYIMRKNGIAAGILLNQLKGDPGLFDEIKPEIPVLQGYNHIRQEEYR
                     EAIPYLREALASKDLPNKRKARIAFVLGQLYDLQHEEAMAYEAYDQVRHFSPSYELEF
                     FARLRGAQSAIASGKRDKAQLLDELKKLSRDEKNADYLTSIYHTISLVNLSEGQRDLA
                     KENLVAGLGEGKDPIQKLESYYLLASMYNEEEDYLNAKKYYDSTLKVMSPKDLRHSEV
                     SRYSESLTDIAKHLGIIQTQDSLLYISTLDEKDQKQWARRMLREREKTANVKVASTPG
                     LTFDKNAFDRADLAATTAQGAGKSSFFAYDDKQLKKGIKDFERTWGNRKLQDNWRIGQ
                     KIAGNLLAADQQAPAAETEEITAGEDLTSILKDIPNTPELRELAHEKIRSSTYALGVL
                     YREKLENHPKAIAVLEGLLTRYPKCNLEQDALYQLYLACLQNGDQVKAGQYLDRLKQE
                     YPGSKYTQSLSDPNWMAGNQNKAAQLQKYYDETYKMFNEGQCNGAMDRIQQVDSLFKE
                     NPFRAKFALVQVLCTGRSQGRDAYINALKDFIARYPQSEERDRAKDMLRYILGDEKAF
                     EVADLQKSAFNPNAFEFLEDELHYVIAVVYDKKESTLSNIKISISDFNQKYFQNDDLR
                     ISNIFLDQEATIPIIMIRKFDSATQALRYFNTVKSNANQFISDQIRHEVFPISQSNYR
                     KLYATKDIESYKEFFNSNYKK"
     gene            complement(8521..9804)
                     /locus_tag="PKM27_02770"
     CDS             complement(8521..9804)
                     /locus_tag="PKM27_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNR06210.1"
                     /translation="MITITEVLALDIRFPTSKDLDGSDAVNTDPDYSAAYVILKTNRE
                     GLEGHGLTFTTGRGNEICVTAIRSLSYLVKGRTLESITSNYSAFWASLTRGDSQLRWL
                     GPEKGVIHLAAAALVNATWDLYAKSENKPLWKLLYDLTPEQVLSCLDFTYLTDEISPE
                     EALAILIKAQEGKEKRLAYLEENGFPAYTTSAGWLGYSDEKLRRLCREAVAEGWKYLK
                     MKVGSDPSNDQHRAAIIREETGPDMKLMMDANQKWEVDEAIRCMKALSRFDPWWIEEP
                     TSPDDIAGHKKIKDAIFPIGVATGEHVHNRVMFKQFLAGGAMDFCQVDVCRLAGINET
                     LMVMILAAKHGIPVCPHAGGVGLCEYVQHLSMFDFMGISGQIENRILEYVDHLHEHFI
                     DPVVIRQGAYRLPARPGCSIEMKEESLREYEFSGQ"
     gene            complement(9922..11058)
                     /locus_tag="PKM27_02775"
     CDS             complement(9922..11058)
                     /locus_tag="PKM27_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNR06211.1"
                     /translation="MKFDILNKPFLPVLAGLFLLFQQCKPVHRDYPVNSKTVTVLGRT
                     VYEEGQGLVLISSMSTVQFSFTGDSVQVFFKNLAPGDDYGYIVYELDVKKVVRVKITD
                     RKEQVLTVYPEIKAPVHVLKIYKATEAQNGDLAVVRIRGRDIKPVESLSKLKIEFIGN
                     SITCGMGADTSEVACGEGKWYDQHNAYYSYASRVARELKMRYTISAVSGVGIYRNWNS
                     DGPAMPELYGSTYFSKERSAAWDFSRESPDYVSICLGTNDFSNGDGVTPREPFDSTRF
                     TETYLDFVKTLYGHYDSARVVLLTSPMLSGEKADLLYRLLLKIADQVNQAYPDRKEAV
                     VFQFEPMKPGGCDFHPSVDDHRIIAFQLVSFLNRYVKQVDPKIK"
     gene            complement(11177..12325)
                     /locus_tag="PKM27_02780"
     CDS             complement(11177..12325)
                     /locus_tag="PKM27_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNR06212.1"
                     /translation="MNKTGIKTGIIAACLFLCGLTQVQALDTLFEVRGFAIGAPKPAE
                     LDRFLKFVDEEMAPRKVNTLILRVDFNYQYESHPELRDSIALSGADVDRIVATCRKNN
                     IRIVPQINLLGHQSWASRTNKLLAVYPQFDETPHVKMPETYKWPNPDGLYCKSYCPLH
                     PEVHGVVFALVDELCDRFQADAFHAGMDEVFYIGDDKCPRCGGIDKAELFAGEVNKIR
                     NHLRLKNRELWIWGDRLIDGKSTGIGLWEASLNYTHRAIDMIYKDVVICDWHYERPDQ
                     TAVYFAMKGFRVLTCPWNRQQVAVMQAEDMARWRKYATNQMKPRYYGMVQTVWSGPSQ
                     FIDGFYGIQTSANNINNSATRNNRSPWDTFRALYKQMDELEMEQKKTD"
     gene            complement(12416..12985)
                     /locus_tag="PKM27_02785"
     CDS             complement(12416..12985)
                     /locus_tag="PKM27_02785"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNR06213.1"
                     /translation="MPDTVHQAPWTYTRMLKELKGDDRYELRNNELIDMSSPSFTHQQ
                     LLQELFQLFYQFVNIDKKGSLILAPFDVILDENNVVQPDLLFVAKDHEDRIRERGVFG
                     CPDLVVEIVSKSSIIRDYVEKKEDYERFGVREYWIIDPQNKFIQVFHLEGNKFKTYSS
                     ADGEEHLKAMSSVLTGLEINWPDLFGSAK"
     gene            complement(13076..14455)
                     /locus_tag="PKM27_02790"
     CDS             complement(13076..14455)
                     /locus_tag="PKM27_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNR06214.1"
                     /translation="MLKIKFGTDGWRAIIADEYTVDNVRRVAFGTAAWMKQKGMSKAV
                     VGYDTRFGGPLFAQATAEVLSGEGMEVILSHDYVSTPMVSLAVLQLKADLGVVITASH
                     NPPSYNGYKLKASYGGPMIPGEIAEIEALIPDEVPAKKAALGKIEKTDLEILYEEKVR
                     AAFDIPAIQATAMPFAFDAMYGAGQRIMKKLFPEAIHLHSDFNPSFHGQAPEPIDRNL
                     TELARTVSTRKDIKLGVANDGDADRIGMMDEHGQFVDSHHLLLLLMYYQHHFKKQQGK
                     VLITFSVTDKVKKLADLFGVECVVTKIGFKYISEIMLTTPILVAGEESGGIAVAGHIP
                     ERDGVWVALTILEFMAKTGKSLTALINEIYSMVGAFKCDRDDLHLSEEKKQAIIKACK
                     ERAYTAFEDYQVQEVEDMDGYKFLFGYDEWIMVRPSGTEPVLRVYAQAADKVKVRQLL
                     DAARNTLLG"
     gene            14718..15416
                     /locus_tag="PKM27_02795"
     CDS             14718..15416
                     /locus_tag="PKM27_02795"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNR06215.1"
                     /translation="MNKKNTTATIAPDHGRLEDNHWHRGLRKKMVEDLRHKGIKDEKV
                     LQAMLRVPRHFFVPKGFESWAYRDVPFPIGSDQTISQPYTVAFQTALLDIRPGERVLE
                     IGTGSGYQAAVLFELGAKVYTLERQEKLYHQTASFLKKMQYLSVRCYLRDGFEGLPKY
                     APYDKILVTCGAPELPQNLLDQLTPGGCLVIPMGDGEDQVMKKITKKSDGEWEEEAYG
                     VFRFVPFLKGLNKG"
     gene            15445..15873
                     /locus_tag="PKM27_02800"
     CDS             15445..15873
                     /locus_tag="PKM27_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR06216.1"
                     /translation="MKYALILIALCFTAGLSGQSNHPLALFNQSIDDAVVKKDTLNLK
                     KWYAEDFIFTHGTGLVEGKSSWLRAVANPNSRFISRQHDSVTVELHHKNIGIVYGKLT
                     VTRHNRENQVHYALWYVRVFHKMKKQWQMVSHRTVQESHL"
     assembly_gap    16461..16560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17097..18008)
                     /locus_tag="PKM27_02805"
     CDS             complement(17097..18008)
                     /locus_tag="PKM27_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNR06217.1"
                     /translation="MYDLIGDVHGHARGLIRLLELLDYKPQKGLYQHPGRQAIFVGDL
                     VNRGPESREVLQIVRPMVQSKHAYAILGNHELNLMAFFTLDNNNKPLYSHSIRNMLQL
                     LPVFESFRSAEAELYDYIDWMYTLPLFLEFEDLRVVHACWHQDSINYVRKHFPGHKFN
                     RDLLIQSLQSRGPEYKAVQMLLKGLEIPLPRNKKGLDAESVRRGFTRVKWWEDHHGKS
                     YRQISTQAKSNLPKYKIPSNLISLDLAYPELAVPVFFGHYSLNGKPNLLKKNICCLDY
                     GVVKNEVVTAYRWNGEMKLDRRNFVQS"
     gene            19834..>21133
                     /locus_tag="PKM27_02810"
     CDS             19834..>21133
                     /locus_tag="PKM27_02810"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNR06218.1"
                     /translation="MIGNLLTGSIPSDLGNIRNLREVFLDNNSLTGSIPASLGNLTSL
                     RFLYLNDNQLTGSIPAELGNLSGLSSMILHNNQLTGTIPSTLGSSLSNLDYLNLSHNQ
                     LSGSIPASIGEIPNILSIKLNHNQLSGSIPGNIGDSPNLNYLFLNNNELSGCLPSQLS
                     VLCEKLVEGDFSGNPELPGGGNLAAFCMDSTGSCCSAGTELNWFLDADGDGHASALVQ
                     ACSAPGMYYTSDSIPVDDCNDMNPAIFPGAPDSTCNGIDEDCDGSADDGFIVVSCQTC
                     TGGTIHYSGPPAPIASVTKQLTCTIPTGTITVSNPTTGHTYSFDNGLSFQTSRIKSGL
                     DSGLYFVKIKSNLTGCISESIQLIINGFPPDPPAPEVIVSAQPTCAIPTGTLKVTDTL
                     PGFKYSFDQGLTFQTSKTKSGLTPGSHQVFAQSIATGCISA"
     assembly_gap    21134..21233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21234..22966
                     /locus_tag="PKM27_02815"
     CDS             <21234..22966
                     /locus_tag="PKM27_02815"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06219.1"
                     /translation="GTITVSNPTTGHTYSFDNGLSFQTSRVKADLQPGWVQIIVKSNA
                     TGCISDFTVVHVDTIPENPAPPQVIVSAQPTCTTPTGTLKVTDTLTGFKYSFDNGLSF
                     QTSKTKSGLAPGSHQVFAQSIATGCISAPTLVFIDSIPTSGPPAPIASVTKPPTCNTP
                     TGTITVSNPTTGHTYSFDNGLSFQTSRVKSGLDSGDYHVRVKSNLTGCISEATFIYIG
                     GLPDNPPAPQVMVSAQPTCDIPTGTVKVTDTLPGFKYSFDQGLTFQSSKTKSGLAPGY
                     HQVLAQSITTGCVSTPTPVFIDAVPTSGPPPPIGGVTVQPTCPSPTGTITIFFPTSGH
                     TYSFDDGMSFQTSRIKNGVTSGTYLLRVKSNATGCLSGPISVTVNELPFVKAPDASVT
                     VQATCDSPSGTIVVSKPTTGHTYSFDDGQSFQDSGTLSGLPPSIYYVRVKSNTTGCIS
                     EGRIVIIDSVFCDSLISGITFPSRIYSLSPTFQNGSLPKSHTGSFKLYPNPASGYFRI
                     KMDQLPSQGKITVRMTDSYGRVLRQFERSITELGNESFQTQDLPRGIIYIQLISEEIR
                     YPVGRLVLVD"
     gene            23649..24269
                     /locus_tag="PKM27_02820"
     CDS             23649..24269
                     /locus_tag="PKM27_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR06220.1"
                     /translation="MFGSLNFLLYIHPRHQFMHRDTTHIIDRIRSGDEDSLREIYREY
                     RQPFIQWIKGEFFCSDDDAIEFFQTAVVILYDNIITGKLTELNSHVKTYLFGIGKNKA
                     HEWLRHRQRQGVHSDELLINYLHEENPEVDEADFAESLRAVDAGMNQLGEPCRSLLSY
                     FYYQKLSMREICERLDYKNEDTAKNQKYKCLKRLQSICADIKGVKV"
     gene            24266..25120
                     /locus_tag="PKM27_02825"
     CDS             24266..25120
                     /locus_tag="PKM27_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06221.1"
                     /translation="MTNTPEHIRETLELYDRGELDPSRLLEVQALLRSDPAWQAAWRS
                     FRSETAGIRLNRLKTNLSVLESLEAELKDPEKKTAAGGDPGAVLTSSPESNHIKQASE
                     TSQPESMEETIDRNTSEGIRFSRLHQALNGLKAEEKKTADPRPAGGRILVIRRIWLGA
                     IAAGIIGFFLVMQLFNNLNRPKYINQYLITHFDEYILHEKSRSKSEKLTFNKDKEIGY
                     DLFVLQEFTEALPYLQKSWLNSNDTLSFFYLTVSHAALGNNKYFREYKSSPVLQSQLY
                     QILLNKIK"
     gene            25122..25880
                     /locus_tag="PKM27_02830"
     CDS             25122..25880
                     /locus_tag="PKM27_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06222.1"
                     /translation="MKLLLISTISFMILYSCEFEGKNHTLLPTQLLETITFDTVISFN
                     PFMSIAQIKTDTFFFFNNDSIEAHKPYSNIVQYDFRAPRNWDFSQPQSVLYKFVSTDY
                     QLSGQGLMPLGHHGNLSLNGVEILAQKGRLCSHRNASFYNGTLYAINKDSLYFIPFDG
                     IYDHEWNCLFSHHGLQKNYIHGFFIRLTKSEFYSATQTGYVQHNINCDGINHIRCINF
                     KSYHTKIGTTLCPRKAIEQGNNNYFVYFFSDIKN"
     gene            complement(25908..28418)
                     /locus_tag="PKM27_02835"
     CDS             complement(25908..28418)
                     /locus_tag="PKM27_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNR06223.1"
                     /translation="MEIASADSLFYGHLLEKAIERYDDLISKSSESLKNYLYFRRNEA
                     AQRLNAHMVLTTELFDHTPIQLSEFSTTKDLLLYFMDEINLYNEIEFGVFTTLCDSIL
                     LNEKVPDYWRAKMDLLRGEYTFYVLNDLYGSYAYYIKANSKFDSLPFITHETVYTKKE
                     LILLTIGQRQYLFGGQLGEEMKDLSIYFTKDTILQSYAFYNSGYIRAIVDRDSGYIKD
                     YNEALLMVKGTKYIKLYQQVLYSFSYSCINRGNERLAELLNNDLMKIVASSDTFVNVD
                     KISAEAFYSNGKYLNTINHSQKAILYLFSLKRFPPSVYLTLSTLLIESYRRIGQLSES
                     TAEAFNNINYKSPIKAFTVEDILMGMADYGYFNFIIVGDIIKNLFLEFKLSGNENKLN
                     MAIRLLDKAEDMINKENYSIEETRRLEVLSYSKSIYEMAMEIYFVMWKSNENVWAFEK
                     FYRCMEKVKASILSGEMNDVKLKLMIPEDVVRKEEFLKNEIRACRMGKKKEAKLDELY
                     FQYDEFNKYLKMNFIEYFNAKTVGEVKSLKEVAIPDTVLFLSIYLIDSNLYILNISTV
                     NQIFKEKVDYRFLQTLDSLIEYQRLGKYDHLEYIRMSNIVYKVILGKLNLDDKKSIVF
                     CPSGKFYNLNFNLLLFPDGKDYLKEHYNLFYTFSASTMKQEGMIQRNDLKTAAFSFSD
                     PNTIRNPTSSFVELPGGYHEILQFKKKANNISYSGLKSTRARFIDCLTNGKYNHLYIA
                     HHGVVISNERENVYLIFRTNTFSEFDTLFGYDLLKYRSKTFQITLAGCETGIGDIIDY
                     EGTYNMVRYFKIIGARRIIASLKKINDVKNEFEFVVFI"
     gene            complement(28562..29866)
                     /locus_tag="PKM27_02840"
     CDS             complement(28562..29866)
                     /locus_tag="PKM27_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR06224.1"
                     /translation="MGNTLKCTALLFLWGFLLAACRTDKTGIDINQYILDTDAAYRSS
                     VRFIADSLFRLHRGKEDSLYAGLGKDEIFKERNNTRYLDIKYLERKLNNRNYSLFDSL
                     YARLAGDSLGILPELQIEWKLLFAVRIGIKGQFENSDRLFKAITGEGERIFLPQYDLM
                     LRIREKHAEMWRYDANNPGKAMAILQVLDTILAGRPGFTGFHRNQLYTLATTHRVLGK
                     FDDALAHALILREKLNGEPVIDSNYLISVSKVLANIYTDMDDPNEANRYFEEHIRYNL
                     SKNRLKAHDLINFAIQLSRNYQFEKADTFLDRALPMVQTPEDSFYYQRTMAFHMMHQQ
                     RQEEARPYLAWVRTYCSNRYDYQLCPSSTYWLGEYYDLTGKPDSAIPLYREAVSHVCG
                     KPFERIQPADLVNKKDVELYYSMLAMTHVYLNILLNNRKIWC"
     gene            30418..30729
                     /locus_tag="PKM27_02845"
     CDS             30418..30729
                     /locus_tag="PKM27_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06225.1"
                     /translation="MINENILLPAMGGFAIQLLSLLELAKVPISQRPDLKSLVYWIPY
                     LVNPLLGGLLGFTYFHNEQTINPVLAIHIGASAPITLRMMASSRPPKTIEPQRSIPEN
                     P"
     gene            31076..31687
                     /locus_tag="PKM27_02850"
     CDS             31076..31687
                     /locus_tag="PKM27_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06226.1"
                     /translation="MIRISRLSSKDLCHFQTRYLDLSGLSIPPGYLRGQTVYGFFRRG
                     QMIGGFILGKEQPLRTTEVFCSPDSRDRLNRSLESKGRVLEICCFWIERSFRKIPWIN
                     AWCWLRMAWAASMERSFDILLFGTNSSGLAKMYGYPKNAFLLHSDQIGGRDTYIFYAK
                     RTHFLAGVFEIVLAKVFRKTRGFRSSTDRGLTAKLQTMKEMCF"
     gene            31744..31869
                     /locus_tag="PKM27_02855"
     CDS             31744..31869
                     /locus_tag="PKM27_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06227.1"
                     /translation="MKNQSISVLLVYLLFGIILLLCLYLLAGLGFRVEALVFEKY"
     gene            31882..32664
                     /locus_tag="PKM27_02860"
     CDS             31882..32664
                     /locus_tag="PKM27_02860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR06228.1"
                     /translation="MESLILDKSWNKLKVSKRIQSSDYDPVFWSHYFHTYDVLLKVIP
                     YRQLLQSLVDAACIRPGHIILDAGSGTGNLAYHLPQGVKTIFVDQSGEALALANKKNP
                     EALTLKTSLTNPLPLEPDSVDHIICNNALYTLPYDQWPLVLDEFYRVLRPGGSLVLSN
                     IREGFSVLRIYREHIRESVRLRGWTHTLAEVLRLFIPTLKMFWYNARIIRADAAGTFH
                     FIHRGEQNSLMLGHGFWPVGPEQAVYAGQAWMAVGIKRYSKF"
     gene            33125..33721
                     /locus_tag="PKM27_02865"
     CDS             33125..33721
                     /locus_tag="PKM27_02865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR06229.1"
                     /translation="MESSACQSVRWRALHLAEKIDEVHQSNQKRINILSLASGHLREL
                     HYVRDFDQKVERFYGIDQDPRSNEEARRSLPYLQLDIREESILNVIREKYKPNRPLDF
                     IYSAGLFDYLNDKVAQKLITSSFRLLAPGGRILVANFAPDLVEQAYMEAFMDWYLIYR
                     DENQMEALAEDLPISQVGEVKTYRDAMGNVVYLQIIKN"
     gene            35119..37479
                     /locus_tag="PKM27_02870"
     CDS             35119..37479
                     /locus_tag="PKM27_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06230.1"
                     /translation="MKKTNLPFTLSFLLLIMGSFLVRAQCPDDLFFNTQEKVDSLAIK
                     YPNCTVLNGDVNIQGGANITNLQGFKNITAIEGSLFLAGNSALASLAGMDNITSISTW
                     FAAYGNGLTSFNGLNKLKIIGASIDIHNNGSLTSFEGFESLTSIGTFAAVNHNNNLAS
                     FEGLSALVSIGHDFNVHDCAQLADFTGLENLKSIGGYFGATRCGTLRNFQGLNSLENI
                     GKYVGIDDNAQLSDLTGLDNLKNVGESFILVRNPSIKSLYGLSKLTHVGHDFYFGSNP
                     ALESTDGLENLSMIGRNLHFDIHAALIKLSGFENLTTIGGHFELWENNNLSDLNSLNS
                     LRSIGGDLSLVRNTKLGSLEGLASLENIGRNLVIDNNIGLTSLKGLGNLKSIGSGPDP
                     GVYIYNNDKLSECAIEALCNVIFTKNQSIYNNGGSQGGCRSQDEIRLACGLAAPCPET
                     AMLKTQKEVDEFVQLYPNCTELKQLVIGDPSGITSDPIVNLHSLHKIQRITSGHLVIF
                     NTRLVDLTGLNNLKSVSRAINISASPLLSSLEGFSSLESIGGGLFLSKLQAFSSLQGL
                     KPGVVNHYFLQIQECPALTSLESLSAILTGNRIRGLFLLNNPGLVNINGLEQLTHLPG
                     PLTIRDNASLVNLSGLKNLVSTGDLITIQNNPVLNYCCAIQPLLNSVKSVPGKINISN
                     NKYGCHSLDEINNACSPSPAMPYFKAVLFATLTASPNPTTGLVRIRASMPTLLLDVVD
                     MMGGSQGISHPAAMDFEIDLGAKPAGMYVFKLFQGDQLKTLSLIKQ"
     gene            complement(37566..38543)
                     /locus_tag="PKM27_02875"
     CDS             complement(37566..38543)
                     /locus_tag="PKM27_02875"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR06231.1"
                     /translation="MQGLDRYTRKLAAILFTDMVGYTAVMQRDEMLAIQMQKRHQAAV
                     DSCVKRFGGEVKNDMGDGSMSILPSAIQVIKCAMAIQEELRKEPRVNIRVGIHIADIL
                     EEENGKVHGDGINIASRIETLGVAGAILFSKEVYNKIKNQPEFKTVLVGSFPFKNVEE
                     PMDIYAIANEGFSVPTSQEALSAGKLKKQDGTLTAEVKNKSYSPWWWLLLIPVLIGVI
                     YLFNRAGKNPEGNSSVLSEPKPELQSWTGRVVHFDSRPAAGVILDINAGVARDTTGPE
                     GQFELLLPTHYQGQQITLSLFYRDKLNLSHEIRLSGPEVLKTLKLKPEE"
     gene            39287..40375
                     /locus_tag="PKM27_02880"
     CDS             39287..40375
                     /locus_tag="PKM27_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06232.1"
                     /translation="MKPRFLTLLLYLWIAGPLFAQQSVTLRGKVVEITPEGTVGVPGV
                     VVSVSGESYDVTAGDGSFKLFAPGGLDQITITIKGSSSMMISPYEGKVSIPPLNEPIE
                     IKLCQEKNEQLINKITQLNTRIRSLQKTQKLTNRQLEQMHRTMLDTITFYQARLDLAN
                     RQLEKAETANQELKNKITELEKTNALLEEKLFLALGEKFTAQQKHFESISTGLNDYIS
                     RLKDLHHILPTDAEACLANTPGACTRFYGYIEKYNQARNQIHEHLEEDLRAVGHFWSD
                     PSVAAGLKEIYGYVLDTIHEPYLFEKMNKTVIQPIKQRSQGNAGLNKTKKQVSEAGHA
                     LAADLEPLIRTLDEKKAFVFNLMTNSIQ"
     gene            40414..41262
                     /locus_tag="PKM27_02885"
     CDS             40414..41262
                     /locus_tag="PKM27_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06233.1"
                     /translation="MLLILIQCFLHSGCNPDCTPLLGLQVLPAVGESGTEVLIKATPL
                     ESLVSQDLEIRFNSKPVTQKKFHPQLGLVVTVPEGVEGQVDLVIANPDCSERLPFNAV
                     PASFFEANKSFIPPSIPQIFIPINPLPPFPPSLENAWLCPENTDYCIWFKFLKDPKGK
                     CTTSIDPTQSFEQSTCNGNNTALLYNKNPIFGVVDKDKNLVHFWIDRTKHPGGNLGTE
                     EFIGSFININDTPYKPFEQLACSPAVGWKRERNHMILVTSTTTNRQLVIYQQAFNDPN
                     FMIDCK"
     gene            41277..42218
                     /locus_tag="PKM27_02890"
     CDS             41277..42218
                     /locus_tag="PKM27_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06234.1"
                     /translation="MFRMHKTVGRSIFLLVFGVFGILTGNAQEQAAEERFSFGNISPV
                     ILPKDGLEVTATGNLTSYWVTSQFGKVVLDRYRISRSENYLNLNYGFSESGRWDLGVQ
                     VRYSRLRLDENARNSPFKVFGSPDATGIGYQSISTVGLRVRALPFENIPEFTLQGAVH
                     FPVAKDMETRSFLGADRIQPELTATYFKNLSPDFYLYLQGRYLLQIANEDHDQTTHFP
                     GLSAFLVKSFLDQKLYVFPGLSWLGAYEQNYQGGRLVSEAQFVMVSLGAQVQPLSYLG
                     VFLNAQRPLYYKSGLSFYSDLVKSSYSDWSLGLRFIL"
     gene            42266..43585
                     /locus_tag="PKM27_02895"
     CDS             42266..43585
                     /locus_tag="PKM27_02895"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR06235.1"
                     /translation="MIPKNDKKLIHAWAMYDWANSVYALVITSAIFPAYYNSVTRNGD
                     HSRVSLFGIQIENTAAYAINLGIAFGIVALLSPLLSSISDYSGNQKNFMRFFCYLGAA
                     GCISLFFFTGSAQVYLGLGGMMLATIGYSGSIVFYNSYLPVLATEDRQDKVSARGYAY
                     GYLGSTTLLILVLFFILNQNRLGISDETLLPRLSFLITGCWWMGFAQISFNKLPRGLN
                     VKPVEKKYLFHGYLEMLKIWRRLKTEKNLRTFLAGFFFYIMGVQTVMFMAASFGEKEI
                     HLNMMNLILTVLILEYIAIPGAFLFAWISKKWGNIRSLSLAVMTWILICIAAYFISTA
                     FHFYLVALFIGLVMGGIQSLSRSTYSKLIPKTGNNAGYFSFYDVCEKMAMMCGLVLFG
                     YLDHASGSMRGSILALALWFIIGLTFLISLFKDAPLPHTNPDRPSIS"
     gene            complement(43578..43913)
                     /locus_tag="PKM27_02900"
     CDS             complement(43578..43913)
                     /locus_tag="PKM27_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR06236.1"
                     /translation="MNKIPIPVHRGYELASADDILYIQADGNFCLVIYQENQFVQSTH
                     SLKELENQLRPLGFCRIHHKYLVNLEHIYRYLKGDGGEVVLRNGSKLPVSRGKKGNLI
                     RELKGQSIN"
     gene            complement(44104..44442)
                     /locus_tag="PKM27_02905"
     CDS             complement(44104..44442)
                     /locus_tag="PKM27_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR06237.1"
                     /translation="MNRIPIPIHEGIEFVLLQEILYFKSNGNYSILFHPEKTKKFTLR
                     SLKELERQLEGRGFCRIHHEFLVNLSHIVRYYKGDGGEVELTNGERLAVSRGKKANLL
                     SALRLNGDLI"
     gene            complement(44487..44837)
                     /locus_tag="PKM27_02910"
     CDS             complement(44487..44837)
                     /locus_tag="PKM27_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR06238.1"
                     /translation="MDTIPERTERVALPCSQGILWMERKRILWLQSEGNYSWAHFADG
                     CKTLLPCLLKQLEAGLSPAGFYRIHHQYVVNLNQLVRFEKADGGQVVLTDGTVLPVSR
                     ARKQGFLQKIYTVQ"
     gene            44937..45524
                     /locus_tag="PKM27_02915"
     CDS             44937..45524
                     /locus_tag="PKM27_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR06239.1"
                     /translation="MNNKIRAENEHILEGLRRGDLRVIEKVFSTCKPAVIQMVVRQGG
                     NEADAEDVFMDAFEAIYNRLQSNGLTLDRASFKTYFIQVCLFQWSKKIRKKNRVHQVT
                     NPAGEVQTGMEEVHEALADLDRKKIVAEKFQLLSEGCRQLLDWFISEDKNISEVALLL
                     GISEGSTRKRKYECKEKLFRLVKEDPMYQELKFPG"
     gene            45521..46267
                     /locus_tag="PKM27_02920"
     CDS             45521..46267
                     /locus_tag="PKM27_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06240.1"
                     /translation="MIEEEWDEAVDRFLRGQMDPEESARFADRLKNDPSLQEYLRLNQ
                     ALHQALRNSPSPFRQTLERINVSSDLRVASKRGWILVVVTAIALSIFAFLGIYLNRKK
                     NSGPEALYLAYMKAPASLSPDLAPDRTGEAQSRSISFQDSVLMETDRLYINGQIDQAL
                     QLLQSTQEDLLSDRMLFQTGLLLLIKNQAGETLRVFEKINAYSPGEILWYGALAHLKQ
                     GEPSAARSKLELIPAESRWYSQSRELLSRL"
     gene            complement(46271..49339)
                     /locus_tag="PKM27_02925"
     CDS             complement(46271..49339)
                     /locus_tag="PKM27_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNR06241.1"
                     /translation="MKFPLREYLFAASLFLFTFCSQPQNPETPAEGMKPLLLDKPASY
                     LQDSTSAFDQFHRAEIYAYMYKVDSALMLAQDCVQRFDHLLQYTADTLTWIYYIDSYF
                     NVGYLYQLKQENDSAFKYLNLSLDWCRKKLGEDHLIATKCLIKLGNCYRNYGELVKSK
                     EYFLKAYEIRVRTMDSLNNFLVNVYNNMASFYWEMGDVDHAEIFASRMQSLVKTLYDN
                     FVIRHSDIPDAKFYKQFMGTNSRPPVVRNLLLSNIPRQYIGATINKVWIHLNNDEPEI
                     ATYLADLADSLIKKHEPGNDFLQRSALDSRAAIHLYHGEWKKAEELMTQFRKSESGAA
                     LVNRHFLRYRLADFFLKTGKYQDCINLVDASLRDPVVSSSNPEFVLLELKARALLKGG
                     QPGDCIRFCKEILDQIHQVPSMDSLVAGLVQWERFTGQEVLRVQDFLKPLIQSMVSIG
                     IQENSINVLEQGYRLLQTFHASMKRLQDELYRRQSKTNRLHYLYPFYEDALELCAHLY
                     EQTGNESYAREAFYISDLVKAFQIKELLDKQNISAYGQEAAPRSQAEQLKTEIAYLRD
                     QILEMERTSPVKDTLKLFNLKKTQAELAGQLVMLAEKEPMETVHVPNPYSLDRFPFDR
                     LRDYLKQEQADLLDYFTGTKHLYLLHMNQSQFRIIKQEANSGLEALAIKFSDALNPET
                     DQTARDSLGVWSAGLYDLLIKPVEDQISGNHLIVIPDGFLARLPFECLQSSIRGFEDS
                     PLGKRTIRYEYASGLLLKEPPLLKPDENYAGFAPRYEGHEIFLQRGLDSLITYPLYAA
                     NREAYGNLLFNEPEVLESANIWKGEAYTGLEVDKSKLLRNGANARILHLAMHAIADDK
                     HPEYSQLILKNPEGPGRNEAIYAYEIARMHLNAELSVLSACNSGKGKYLKGEGVMSLA
                     RAFKAAGCPNVVMSLWQANDASTKDLIVSFFKHLKTGLGKADALRKAKEDYLANAIPE
                     LKHPYYWAGLVLVGDNEPLILEGMDSISYWILAALLAGTVLLAFWLRRQT"
     gene            complement(49477..51078)
                     /locus_tag="PKM27_02930"
     CDS             complement(49477..51078)
                     /locus_tag="PKM27_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNR06242.1"
                     /translation="MKKLLFLTIVSVLLVRCNTDELQDLNINPQAVNQIDMNFLFSAA
                     LLGIASNGSSGDNRYIDWRTNIGLCAHAIQHLGNANSGGISPGDKYTDNGETRAAPFE
                     MSYRDQLKNIAEVLKQTGDGGFDAGNKQNTRNAARILRAWTYARLVDMYGNVPYSEAN
                     LGTSGIFFPKYDDGKAIYTDILKELDEAAAALNASDKDQASFAKADFVYKGDVTQWKK
                     FAYSLMLRLALRAADADGALAGTYAGKAVTGGVMTSNADNFVVPMALSPSEWTNQNGI
                     SRAFYPGDGGQSPYLGQTLINFLKGTDPNSVADDDPRLMIISGGIGNWTANDFTIIGD
                     GANPLNQKGMPHGLDKTDVDALEGRNVDFNTTYSKINVKLMQDDEPYVIMRYAEVEFM
                     LALAKLKNLGGVAGTDKEHYEKGVKAAMQEYTIHDASLTVSDAAVTNYLSIYQYGVTK
                     PAIQMIADQLWVANFLNWYEVWSYWRQLDFPVLVAAKHPSNITNGTIPVRIPYPTSET
                     ASNPNLATGGVTPDTYTTKVWWDTK"
     gene            complement(51097..54300)
                     /locus_tag="PKM27_02935"
     CDS             complement(51097..54300)
                     /locus_tag="PKM27_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNR06243.1"
                     /translation="MIKKTLLILLVFCGWQAIAQRTITGKVTNGEDSEPLVGVTILIL
                     GTTQGTVTDFDGNYSLDNVPDNASLRFTYTGFQEQVIVVGNQSSINVTMQPGVALSEV
                     VVTALGVSREKKALGYSVSEVGGDNFIQARENNIANALTGRVAGVNVSKVASGPGGSS
                     RVIIRGNKSLNGSNQPLYVVDGVPMDNSGFGQAGLWGGSDEGDGMSSINPDDIESITV
                     LKGANAAALYGARAANGVINIITKRGAKRKGIGVEFNSNFVFETLNDLSDLQNDYGSG
                     GFTGALPNGVSTKPSTIQEAGNWGHQGWGPKFDGSSVIQFDGISRPYSYAGDRFKEYF
                     ETGTSFTNSLAFTGGTDNSSFRISATDLRGSTILPNTGFDRTNVSLAANSKFGKRITM
                     DAKILYSLENNKNRPTLSDSPGNGIQNVWKVAPNIPYSVLRGDPNKIGAIPAEGIPAN
                     VLSYWGRIPGEELQVNPVNDQWSSGGYWAAYQFINNDKRDRVISSGRLRYDITDFLYV
                     EGQIGLDKYARRAKGLTPQGTGYNRAGAISEGIDRVQETNFQYTVGFNKTFGDISVNV
                     FGGGNRMRRESDVINISSDGGFNVPFQPFINNSVRRNWGFGQSETGINSLLGQAELGY
                     KGYLYLTGTVRNDWFSVLNPEFNSILYPSVQASFVASDAFKNLPSFISYLKLRGGWAQ
                     VGNATVGAYSTAITYGLNGAPHVGTPLGGFAFAIGQGGTIPNPNLKPLTSTEIEFGVD
                     IRLFQNRVYADLTYYSQKTTDDILPATISRAAGFDATAVNLGKLTNRGFEALIGVTPI
                     QGKVTWDVSLNMAKNNNEVVSLIEGSKELVLEEPRTRNVFIKHIVGYPYGMITGRVQK
                     TDPSGNLVFDQWGSPIGDPNYQIIGNGVADLTGGLNNSLTWNNFNLGALVDFKFGGDL
                     FSGTNMRLTQWGYHKQSLIGRQGEAPLHVKGVIERSPNVFEPIDRDLTPQEAQRYWST
                     TGSEQNGKADMYVYDASFIKLRQLTLGYNFPNKMLSKTPFQNLSLSFVGRNLAILHKN
                     VDNIDPESSYTVSNSQGMDYFGFPSTRSYGFNLKVGF"
     gene            complement(54564..57917)
                     /locus_tag="PKM27_02940"
     CDS             complement(54564..57917)
                     /locus_tag="PKM27_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNR06244.1"
                     /translation="MKYFLFLLLVFSGSISFEVAPSSAPPEKKLFTLLSPDHTRIRFN
                     NQIKDTKEANITIYSNFYGGAGVGIADINNDGLQDIFMAGNLVEDKLYLNKGNLQFED
                     ITKKAGILSNGGWSSGVLMEDINKDGYIDIYITRELYDDKPELRKNKLYINNGNSTFT
                     EKSEQYGVADSARTRHASFIDYDKDGDPDLLLLNQPPNPGDYSSYYGTELIKEEYSIK
                     LLENLGDRFVDITEKAGLKKTGFPNSVSASDLNGDGWTDLYISNDFWIPDWLFINNGD
                     GTFTNKIHDNLKHISYFSMGVDAGDINNDGRLDLVVVDMVAEDNYRLKANMSGMNPAA
                     FWKVVQDSGHYQYMFNTLQLNSGGAHFSDIAQLAGTATTDWSWSSLFADFDNDGWKDL
                     FITNGLMRDIRNKDAHKAFVDNIQAAMAEYMAEHPTLDQKVSIWDIVDINKALSLTPS
                     VKLRNYIFKNEGDLRFSKQMEEWGLTEKTFSNGAAYADLDNDGDLDLVVSNINDLASI
                     YRNNATENNHYLRIQPVADAGNITAMGTKIWIETTAGPQFFEITGVRGMYSTSETIAH
                     FGTGKNPIIKKVKVRWPDGKENLFTDVKADQVLKINYSTAAFPEPETKTVPAPLFTEI
                     NKEINFSHRHIENSFDDYKVQVLLPHKMSSFGPVLSTGDVNADGLADLFVGGAAGTPG
                     VLYCQKADGSFEELINDCFTRDKNHEDMGSAFLDFDLDGDLDLYVVSGGNEFRPGSKS
                     YQDRIYVNDGKGRFTKGEGILPELLFSGSKVRPCDFDGDGDPDLFIAGRHTTWAYPVP
                     ASSALLKNDQGKFTDVTAAMAPGFKDLGMINDAVWTDLDGNQKPDLVLAGEWTPILVF
                     LNKGDQLVRNTDDFTLQSSTGWWFSIKAADMDRDGDQDLIAGNLGLNYKYKASEEEPF
                     EVYYYDFDDNGSKDVVLTYYNFGVQFPLRGRQCSSEQVPALSQKFPTYDMFASSNVAQ
                     VYGKDKLKNALHLEAKTFASTYFENLGNGLFRPHILPTMAQLSSVNDIITEDFNKDGH
                     MDLLLAGNLYQAEIETTRNDAGYGMILLGDGKSGFTALDRIKSGIFMPWDVKSLASMD
                     SPSGRVILAGCNDDILRVFRQNPAEKKGK"
     gene            58021..59763
                     /locus_tag="PKM27_02945"
     CDS             58021..59763
                     /locus_tag="PKM27_02945"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HNR06245.1"
                     /translation="MMVPRCVLMLFFCPPFLFKVFATGPAVLFFRANADTVAKFEKFE
                     ISLDFKADFTNPFDPAQVDLRASFTSPTGKVWQIPGFYNYSSWTAVWMIRFSPDETGT
                     WKYTVSIKDKTGVNDSINRVFQVRPSAHKGPVKIAANKRFLQHANGDSYFGVGLWYND
                     SYAAYNQGRINPQELDELKRLGVNFISTFITPLETHGSGLGRYDQSICGRLDEVLQLC
                     EERDIQLSLNIWFHSYLSETVWGGGNIRWNTNPYKQITSAKDFYRSEEAWKFQQLLYR
                     YMIARWAYSRSLLLWFVIDEVNGTDGWVSGDSLMAAQWGKKVHDYFKAHDPYGHLTTG
                     TRSGGIGEFWHEGYQIFDLSNREIYEAQGFPIVRSGRLDSADTHPLALSYMNYAGQIE
                     RLWKGYEKPAIIGETGWDHTFYEPSMPGYQAMYHNALWVTLAKGTAMTPFWWAHSGYL
                     NDNIITNQVRNIARFSQEIPFAKLSKPKPVEAITGGSNAYAMSSDQLIYGWVVNPDSD
                     VSGAQVSLTLTGSPASKYKVRIYHTWRGAFIQESEVVPQGNQIRFEIPVLKIQGGRGF
                     YIGQDAAFILSPIE"
     gene            complement(59834..61660)
                     /locus_tag="PKM27_02950"
     CDS             complement(59834..61660)
                     /locus_tag="PKM27_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNR06246.1"
                     /translation="MTIERVFEFFDYQLKHYPQEKSFGSRQNGEWKFYSTAQIIELAR
                     QLAQGLLDSGYEPGDNMAIISYKNQPEWIVIDLAIQYAGMVSVPLYPTISPGEYEYIL
                     TESACKAVFIGKGDLDQKIGNVRANLPALKQIIGIDEGVGEIHWKDLLRSDRTGEVDQ
                     SIARIKPSDLVTIIYTSGTTGKPKGVMLSHFNLASCIREVKDILPVSCGEKVLSFLPI
                     CHIFERAATYAFVYVGLNVYQTGTDNLGGDQGDLKSVSPHFFTCVPRLLEKVYEKIYN
                     KGSDLTGIKKKLFFWAISLTEEYEFDFKPTGLKAIQWAIADKLIFSKWRAALGGQVKG
                     VVTGSAPCPTRIIRVFSAAGIPVREAYGLTEASPGISISLFEPHKALIGTVGPLMSNV
                     EVRLDNSEAIYKEGEGEILASGPNIMIGYYKHPEWTAESLKVIDGKTWLCTGDVGTFV
                     TGKDGIRFLKITDRKKELFKTSGGKYVAPSPIENKLKEDFLLDNIMLVGDRMKFVTAL
                     IVPAEESLKNWFEEEKIPWTGLKEAVKLPEVIKLYQQIVDQYNPLFGHIEQIKKFTLV
                     PEVWVPIKSDGREGELTPTLKLKRRLLLEKYKDAIERMYVEG"
     gene            complement(61671..63101)
                     /locus_tag="PKM27_02955"
     CDS             complement(61671..63101)
                     /locus_tag="PKM27_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR06247.1"
                     /translation="MQAEGFRLWIDRNGFPVFVLISALAGIVFQETWCFLAPLFLLFF
                     QISVHRLHWIFYLLLISIPLSTEVDLPGGFATDFPDELLMWWLFGFGLVYVLHPATAD
                     IRKILKHPITVALAVHWLWILFTSLSSQDVWVSLKYWISKTWYLFVFYILAYLFLKNE
                     KRLFRFALLSIGTAALTVMIIEWKHAGSGFAFDQINTAVRPFYRNHVNYACLLVCLLP
                     YAWPVWHLLKRKAFFRMVWIGLVLVLLAGIFFSYTRAAYLALPAVILVYPVVRWKWVN
                     RAMLILPALGIAAMIYFTSGNRYLELAPDYNKAVTHVEFGTLLEATPQGKDISTMERL
                     YRWVAGVRMVGEKYLVGFGPAGFYSAYPAYTLHRFSTYVSENPDRSGIHNYYLMTAVE
                     QGLPGLIFFLVLIWLFFLEAQPLLRAKLPALRHWGFAATMSMSSILVILFFNDMVETD
                     KVGTYFFLNFAILLRLNQIRILHESN"
     gene            complement(63107..64198)
                     /locus_tag="PKM27_02960"
     CDS             complement(63107..64198)
                     /locus_tag="PKM27_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06248.1"
                     /translation="MEEPEQIRDAIRILWDKKKPIFTWALLVGICAALLSLTLKNYYK
                     ATTVFYAASPDLAKPELIFGNSLSDPEFYGSGEDLDRLLTLCHSNEIKDHLISTFDLY
                     RHYKMDSTRPFAHHKIRKKLDKRMEVVKTKYDAIELSIEDQDKALAASMSNEARESVN
                     RAAVKLITDRLSAVSVTYRASIQEKQALLHALNDSLYRLRKIYPIYNIEAQTESMSAI
                     AAQVSSDLAGEQARYEVLKKSNAPRDTLMYLAARIKGLETQWRNITRSNAGVASFSLE
                     NFNEGVSRIQSLDLNIERLNAQLNEDRIRYQHAVNTAGAAAPALITVSQAEIPIYKSR
                     PTRSLLVIAAALFSALSLSLYYILKSFWK"
     gene            complement(64239..65741)
                     /locus_tag="PKM27_02965"
     CDS             complement(64239..65741)
                     /locus_tag="PKM27_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06249.1"
                     /translation="MPSSANVFLNLNDTFADTPVRMAIYRREFVLNIGFLLLANLLIK
                     PFYVLFIERDFQNQAGNEAWGLYFTLFSLSMIPQILLDLGMTVYVNQKISSDRSAALE
                     IWKNTAWLRPFFALAFLVLYFLLAGNSMRSQPFLILGIAINQILLAGILYLRAFVSGL
                     GHYRLDSLFSVADKLIFILLALFIFPAFEGQPVFIFLSIQFYSLVIPLVTGLFWFWFK
                     TKPGLRMGTLPAAIGILRQCFPYAMIFILMVLFSRMEPVWLNGLRNDGAQQSGIYAAA
                     YRLLDAANMLGFLFAGILMPMFAHATGQKLWEQRQSLFDLAWKLMIVLASLIAMPLAF
                     QHRYIMQALYTDPAEDILHLTILNLVPLTINYLLSTLLTAVKEAGAMNRLFLISICIN
                     AAGHLLVTRSYGAAGAAGVALVTQTITAFLLAGLIHLRKIIRFQWKHFGLMVLVLGMA
                     VLTGTGVLYSGLPAPAAIPVHVLALLILVFSSNLLPLAQVFRLVFKPGVD"
     gene            65808..66611
                     /locus_tag="PKM27_02970"
     CDS             65808..66611
                     /locus_tag="PKM27_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNR06250.1"
                     /translation="MFKSRLLLPCILISLGLSAQPQRGFEAGPWLGGAHYFGDLNTTY
                     KINRPGPLGSLALRYNFNQRLAAMAKLNAFRVSADDALSTNAFEKNRNLSFFSTVFEG
                     SAQFEFNFFPYKHGDKERYFTPYLFAGASAFNYNPKTRYNGAVVKLQPLGTEGQPIGE
                     EYSLFQPALLYGMGFKYDLSPSWSLNIEISARTLYTDYIDDVSTVYPSPDQLANRDPL
                     SAILSDRSLQADTEPGRQRGDRRESDGFGMLSIGLMYFFNPLPCPKILK"
     gene            66790..68562
                     /locus_tag="PKM27_02975"
     CDS             66790..68562
                     /locus_tag="PKM27_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNR06251.1"
                     /translation="MRQIITLLYFLTALSLHAQVHIRTNLVGYQPADHKSAIAFTDLD
                     LSGEFQVVDVSGKLVFSGPILPAKAPGYGPFKNYYELDFSGLTAGGQYQLVIPGRLQA
                     TSPSFHIDPGLYKNLPDVLLEFMRQQRCGYNPFFDHVCHQKDGRIMDGPIPDSTYVDV
                     TGGWHDAGDQLKYLITGSNATARMIMAYQHFPQVFKDQFNALGQPGANKIPDVLDEAK
                     WGLDWILRLHYAPGHLIHMVADDRDHVGWKLPMYDPSNFGWGKDSYRVAYYATGKPQG
                     LGKYKSQATGIANVAGRSAAALALGHQVWKQKGYDPAFALKCLQAAEDLYRMAKQQAG
                     YQQGNSYGAPYRYNEDTWTDDMEWAAAELYRATRKPSYLTDAKTYATQTGELSWIQHD
                     TSAHYQFYPFMNIAHYALYNLVDPAFKAKLAGYYRNGIKQTLKQRAGLNAFQHGVPFI
                     WCSNNLLIDLVLQIHLYEKMTGDKQFSAYKLNMRDWLFGRNPWGSSMFMNIPTQGPYP
                     RDVHTSTWALTKKEVPGGLVDGPIYYTTYQKQIGLQLTHADPYAAFQNGYVVYHDDIG
                     DYCTNEPTMDGTACAVFFMALFTE"
     assembly_gap    68591..68690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            68810..71200
                     /gene="lon"
                     /locus_tag="PKM27_02980"
     CDS             68810..71200
                     /gene="lon"
                     /locus_tag="PKM27_02980"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNR06252.1"
                     /translation="MFDSLKFSAPEEIEFPLLATEEEEEPSRETDEPEILGVLPLKNT
                     VQFPGIVLPITIGRDKSIQAVNEAYKHDKRIAVVTQKEAGNEDPNFNGLYRIGTIARI
                     LKVLKMPDGTTTAILQGRKRIKILEELTEEPIMKARVAALVYKPVSNKLEFEALIATI
                     KDLGKRIIDLSPHIPGEAATMLRNISDDSFLLNFIASNLGVNLQQRQQLLEMNDLLKK
                     GQAILEYMHKDLQLLELKEQIESKVRGDIEKQQRDYFLNQQLKTIQDELGQNPQEDEL
                     KKLEVRAAAKKWPKAAKDAFDRELAKLRRSNPQMAEYGVTLNYVEFLLDLPWQEYTKD
                     NFDLNKVKAVLDQDHFGLDDVKERILEHLAVLKLKGDMKSPILCLVGPPGVGKTSLGK
                     SVSKALKRKFVRMSLGGLHDEAEIRGHRKTYIGALPGRILQSIKKSASSNPVFILDEI
                     DKLGKDFRGDPSSALLEVLDPEQNTTFYDNYLELEYDLSKVLFIATANSLQNIQPALL
                     DRMEIIELSGYSTEEKIEIAKRHLIPKQLAAHGLKPKHIKWNDEVIAQVIDNYTRESG
                     VRALDRQIAGLMRRVAKEVALKSSYSITLTKADVTRMLGLEKVNPDTYQDVTMPGVAI
                     GLAWTRVGGDILYIESSLSKGKGKLTLTGNLGDVMKESATTALSFVKSKSKSLGIDEN
                     IFETHDIHIHIPEGAIPKDGPSAGITMLTSLVSAFTNTPVKAHLAMSGEITLRGKVLP
                     VGGIKEKVLAAKRAGIKEIILCRENEKDIREIKPEYIAGLQFKYVDRMEEVIGLAI"
     gene            complement(71247..71816)
                     /locus_tag="PKM27_02985"
     CDS             complement(71247..71816)
                     /locus_tag="PKM27_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNR06253.1"
                     /translation="MKNLFIVILVALNFSGLAAQNFFELDKSPLDMVYYPNDYAHDRR
                     FAPQRIGSDTAMIRVIYSRPAKNGRELLGKLIPYGKVWRTGANEATEIKFYRDLLFGG
                     MELKAGTYALFTIPETNEWTIILNSALDHWGAYSYKAEQDVLRIKVPVRTNTKTLENF
                     SIVFEKVDDKNAIMYLGWDTLLVSIPIGL"
BASE COUNT        18547 a        18280 c        16539 g        18396 t
ORIGIN      
        1 ggcccacatt gtaatggctt gataaacaga attttatcag gccattttta tttgggcttt
       61 aaaaatacta cctgataatc ggaaggatcc aaaacaaata aaccggacat aaagctggac
      121 ataaataatc ttgaatacca tatccgatcc aaacgggtgc agggaatgtt ctacccattt
      181 aattgaaatg aaaatcccct tcttcaagct cagctataat aagctaaaaa aatcattagc
      241 ccgttaaaaa cgattgcagc cataatacgc catgtatttt tactatagtt tatatccctg
      301 agggcagaat tcagccgaag catataaaaa cttaaaccca gattaatgat tgctgcaaat
      361 aggatcccaa ggtaaataat atctaattta atgatgtgtt tattagcgcc ataaatcaca
      421 tacataaata gaaatacggt ggttgtataa ctttttaaat ttgtaaattg taatgatttt
      481 ttctgatact gtgcttcttc catattagga aatgaattca aacaatagtt aaattttttt
      541 tatcttctta tttttaaccc aacaagaaca aactcaaaat taataaaacc ggatctgcct
      601 cagcgactgc aaaggagaag gaatactctc aaaaacatcg gccgccaggg ggtcaatggt
      661 cctgagctta ccaaaagaaa taccggcctt cttttcgatc cagcggatgg gcacctggta
      721 agtctgaaat tccccgaaaa taaactcttc catattcaaa taatcttcct gggaaatggt
      781 gtaacccgta gcgcagagtt tgccggtatc atcgtggatg aaggcaatca ctttccagaa
      841 agccaccgga atcctgaccc catcccggac cggatcattg tcatgaaaaa acggccctgt
      901 aaacaccgaa attttttgct tgtcctgctt agaattctcg agggcatagt tctccaaagt
      961 cagccatact ccgccattaa agggttgcat ctgcggcacc acattggtga cgtgcatgga
     1021 atcttcgttt cctgcactgg ccaggggccc ccagttggga tcctccctgc gggtcatatg
     1081 tccgcgggaa aacctggggg cgtcaccata acattcgttc atgatctgtg ccccctttgg
     1141 gatccgggaa tcatacctcc agccgggtcg cttcaagcgg acatactgct tgccgtcaat
     1201 atttaccgcg ctatagaaac acaggcgcct ggatttgctc ataagcactg aaaaatgggt
     1261 gtatttgatc accgattcct tcttattctt accaaatttt aaaatatcgg atctggtttt
     1321 caccgtgggc aaagagaccc tattaccgtc caggaaattc aattcatatc ccttccggtc
     1381 ggagtattcc ttcacgggaa aagccttctc ctgtacaaag ccgtgctcca ccaggacttt
     1441 agaaccctgg tcgctgaacg catgaaagtt cttaatattt aatttggcca gggtttccag
     1501 tatttccgct cctttcacgg catagttcgc ctccagcaac aatccaccaa agtgaaggcc
     1561 tatggctccg cccgtttcca tatcctggac cagggaacct gaattcccac ccagggtgga
     1621 tgcgtcgtgc agaaaatacc acttgctcct gggattgccg atgatctgac ccggggcata
     1681 tcttttaact ccgaacacat tgccgaaaat gcggcgttcc atttcaggat ccgtcacgcc
     1741 cctcggatcc tggaatgggt aaccgaccac cccgatgaat tggttgaccc tcaatttatt
     1801 tgagataaaa ggaatagggt cgggaagacg gttcgatttt ttaatcttta ataaagcgat
     1861 atccggcgcc cctttgatgc tgtctgccaa aaacaatact ttttcaatcc ggatctcgaa
     1921 ttggtttttg gccggggtat ttaaggcgta ctcctcctta aaatcaataa ctgcctgaat
     1981 ggggtcgccg atgaaattgg gcttgaagga cgcgcccagt gccgtcggct ttgacttgac
     2041 ggcaaaaact tcggccacat ggcggttggt aactaccacc tcatcggaaa ccagccaccc
     2101 ggtcccgata tttttaaact ggtgattttt aagctcaatg cggcccacgg attttaaggg
     2161 ccgttcgacg atcggccggt atttgatcat ccggtctttg agttcattgg attccggcaa
     2221 ttcaattttg ccgtttttta caaaaagaac gggtcgcata taaggcacta cgacggcctc
     2281 cacctggggc aaatggtccg ccttatcaaa atcttggatc acggccctga ccagtttgga
     2341 gtcaatattc ccccgggtcg cggtgggcag attttggccg gaattcagca gttccttcat
     2401 ttcctttaaa aggttttctt ctcccctggc gatggctttc aagcgattca taaggcgtga
     2461 atttttaaaa gttctttcaa atgttttagg tgcgatccct gcctgaagca ggtgtaagca
     2521 gcaggtaaaa agtcactcca ttaaaacgga ataccatgtt ttttttatgt aagccgggct
     2581 tctttttaat taatgaagga gtccccatct ttgaatttat aattaaaaag caattccttt
     2641 ttggtttcac ctgatggtgg aatccagcct cggccaaata aactcttcag tcctaatcct
     2701 tggtaaaaac caggctttca acgcttacca ggataatgaa ctttggattt ttgcattccc
     2761 ctgtgaccag gatacttcag gagtgtaaag ggtgttaaaa cttttataaa tgcagggtgg
     2821 cgggcaaaca gagctgtttc agcctctaaa ttgcaatctc aatatacgat cttcatgact
     2881 tattcttatc tcgaacggac cacggacgcg gaacaaatta agatgcgaac cgaatccttt
     2941 tcatcccatt ccgatgaagc cttgcggcat atagtccagg aagcccggga aaccggaggc
     3001 ccacattctc atgaccaggc cctggacctg atcgccctcc accccatcct gatccgacga
     3061 tttgggaaaa gcccggttca gatggaaggg cgaaaattta taaggcttga tcctgaacca
     3121 ttacccgatc cggcaaaaga ggaaaaactc cggcaattca ggagcaaaca ccagcaaatg
     3181 ctctccgccc tggcccgcaa agggtatgat caactgaatg atgaagagcg ggcatactgg
     3241 aacagcgttt attaaaacaa aaggcccggt cctgcgcagg ggattccctt atgaattgat
     3301 caccttcttg atctccacat ccttgttcct cagatcgatc cctccctcca aaatggactt
     3361 caggttggcc agataaaagg tccatccctc catgcaaccg aggtgataca tgaatttgga
     3421 ttcctcatcc gtgggaatat ggtattgggt cagttccacg atggtctgcc ctttctcccg
     3481 gctgaggtcc accctgacct gcatgtcgtt ggatttattg cccgcatcaa aagtaaaggc
     3541 gaggcggtcc tggccgttgg catcgagaat attgccgtaa tgggtcactt catcgcccca
     3601 accgtgccac cgccaggtat aattgtcccc cagctggatg taatcatggg gaccccgttc
     3661 ctcgccatcg gcccgggtaa acaaggaggt ccgtaaaaac caggattcaa tgccatcccg
     3721 cgtggcccag gcggtataaa catccgcaag cggggcatgc acgggaatgc gcttggtgaa
     3781 ggtggtccag tcgtattgca tgtaaacaaa tataggtgga gatggaagga aaaacaagaa
     3841 gggtgggtgg cgaatggtga gtggtgggtg gtggatggtg gtctcaacct taaccttaac
     3901 ctcaacctta acctcaacct caacctcaac ctaatcctta aacacctcta tttccttcgt
     3961 ctcgatgatc agctcggtaa acttgccttt gaagcgcgtg gcgcgcacaa tatgattgtc
     4021 gatccagtgg tagttgcctc cgcgcggttt acccatgagc atgccgtgat atttaaaacc
     4081 gtgtttgcga agccattctt cggtgatctg ccggtgctct tcggtgcggg aagtgaaata
     4141 atagatgata tggccctggt cgtaccattt gttgatgatt tcgagcgcat cgggataggg
     4201 cgctacatga cgcatgcgtt cgggctcttc attgggaaca tcgtcacaaa tggtgccgtc
     4261 gatatcgatg aggaaatttt ttaccccttc cggcagaatc ggactcaggg cgttcccctt
     4321 ttcatcgtaa atttttttta gcccgggcgc ttgattgtca tccatgaatc ggtaataaaa
     4381 atgaggcgca aaggtaacct gaattcttaa aattttatat ggtcctcggg gttcacggct
     4441 ttgccccggt accacagttc aaagtgcagg tgcggcccgc tggttaggtc gccgctgttg
     4501 ccggcaatgg caacggcctc cccggccttt acataatccc ctgctttttt cagcagttta
     4561 ttgttgtgcc ggtacacgct gatgatctca ttcgggtgct ggatcaccag gctgtttccg
     4621 gtctcctggg tccagtctgc ggcaatgata ttgccatcca tggtcgcctt cacctcggtg
     4681 tttttgggaa cgatgatgtc tatacccagg tggcttttct gactggacat tttttcactg
     4741 acctccccga ccaccggagc gataaaaaag agctgttcaa tgggcagtga ccggctcaca
     4801 ttggacaagt tggtgttttt tacattggcg atctggtttt caagcagctg ctcgtcttca
     4861 agggccattt cttcttcact tttgggtacc ggggtttgat tggccggaag ggctttgatt
     4921 tcacttttat attggtccgg attagggatc tccacttccc cgttgatcag cttctgcatt
     4981 ttgctgatat aaagctgctg ggctccgatt tccgtttcca tcctttccac ttttttgtat
     5041 aattcgatgt aatcgcccga actctccgag gcgaagccgg ggatcagcct tttcatggga
     5101 gtaaaaaata aaagcagggt gaccagcagg gccccgacga ccatcagcat gctgagcagg
     5161 acatatacat tcagcaggtt gagttggtag gagcccacct ccttataagt ctcgtggttg
     5221 agaatgacca tcctataagg agtttttatt ctatgtaaaa ctctttgtat cagtgtcttt
     5281 tgcatgaagc ggttgattta ctccaaaaaa taaaataaat ttgccgatta ttggtttaat
     5341 acaggaccat tcatccattc cttttatgaa actgagcccg attagccgtt tgtccctggt
     5401 cgccctggcc tttttggcca acgcctgcgc ggttcaaaaa agcaaaaaag acatttccgg
     5461 ttttgggctg ttttaccaca atctcacggc caaatataac ggctatttta atgcaagaga
     5521 gctggtggac ctcagccagg aaaccttaac caatcaatac cgcgacaatt tcagcaagat
     5581 cctggagctc tatgaatata atggagtgga caatcccgcc gcggccacgg ccaatctcga
     5641 catcgccatc aaaaaagcga gcaccgtgat caacctgcac cggcccagcc attgggtggg
     5701 ggacagctac ctgctgatcg gacaggccca gtacctcaaa caggattacg aaaccgccga
     5761 agagaccttt aaatacatgg tccagaacta caataagctc aacgaaggca ttgaaaagaa
     5821 aaagtccggc tcccgctccg ccaaaaccaa agaacaaatc aaagcccagg aagaggctaa
     5881 aaaagaaaga gaagagcaga aaaaagacct ggaaaaagaa aggaaaaaaa agataaaaac
     5941 cagggaacag gagaataaag aaaaagcgaa agcccgggcc caaatgatca aggaccggaa
     6001 aaaaggaatc aaaaccacgc cccctgcaaa atccgccgtg aatgccccca agcccaaatc
     6061 aagcgccgac tctaccgggg ctgcggggac ttcagcttcc gccgttaaaa aaacaagcgc
     6121 tttaaaaaaa gcaaaagaag aaacagagga aaaaaccggt actccccggc ccccaaaagg
     6181 caaacaccgg ccggtactcc aggatggcca gctttggctt gccaaaacct atatcatgag
     6241 gaagaacggc atagcggccg gcattttgct caaccagtta aaaggcgatc ccggcctgtt
     6301 tgacgagatc aaaccggaaa ttcccgtctt gcagggatac aaccacatcc gccaggaaga
     6361 ataccgggaa gccatcccct atctccgcga agcccttgcc tccaaagacc tgcccaataa
     6421 aaggaaggca agaatcgctt ttgtactcgg ccagctctac gacctgcagc atgaggaagc
     6481 catggcctat gaagcctacg accaggtgcg ccacttcagc ccaagttatg aattggaatt
     6541 tttcgccagg ctccggggcg cccagtcggc catcgcttcc ggcaaaaggg ataaagccca
     6601 gctgctcgac gaactgaaaa aactgagccg ggatgaaaaa aatgcggatt atctgacctc
     6661 catctaccat accatctccc tcgtcaacct ttccgaagga cagcgggacc tggccaaaga
     6721 aaacctcgtc gcgggcctgg gggaaggcaa agaccccatt caaaaactgg aaagttatta
     6781 cctcctggcc tccatgtaca atgaagagga ggattatctg aatgcgaaaa aatactacga
     6841 cagcaccctc aaggtgatga gccccaaaga cctccgtcat tcggaagtca gccggtacag
     6901 tgaatcgctg accgatatcg ccaaacacct cgggatcatc caaacccagg acagcctttt
     6961 gtatatcagc accctggacg aaaaagacca gaagcagtgg gcgaggcgga tgttgaggga
     7021 aagggaaaaa acggcaaatg taaaagtggc ctccacgccc ggcctgacct ttgataaaaa
     7081 tgcctttgac cgtgccgacc tcgcagccac cacggcccag ggcgcgggca aaagcagttt
     7141 ttttgcctac gacgacaaac aacttaaaaa aggcataaag gattttgaac ggacctgggg
     7201 caaccggaaa ctgcaggaca actggcgcat cggccagaag atcgcgggta acctcctcgc
     7261 tgcggatcaa caggccccgg cggcagaaac cgaagagatc actgccggag aggacctgac
     7321 cagcattctg aaagacattc ctaatacccc cgaactcaga gaactggcgc atgaaaagat
     7381 aaggtcaagc acctatgccc tgggcgtgtt gtatcgggaa aaactggaaa atcaccccaa
     7441 ggccattgcc gtcctcgaag gcttgttgac ccggtatccg aaatgcaacc tggaacagga
     7501 tgccctctat caactctatc tcgcctgtct ccaaaacggg gaccaggtga aagccggcca
     7561 atacctcgac cgattaaaac aggaataccc cggcagcaaa tacacccaat ccctttccga
     7621 tccaaactgg atggccggca accagaacaa ggcggctcaa cttcagaaat attacgacga
     7681 gacctacaag atgttcaatg aaggccaatg caacggggcc atggaccgca tccagcaggt
     7741 ggacagcctt ttcaaggaaa atcccttccg ggccaaattc gccctggtcc aggtgctctg
     7801 tacgggcagg agccagggcc gcgacgccta tatcaacgcc cttaaggact tcattgcccg
     7861 ctatccccaa tccgaagaaa gggaccgcgc gaaagacatg ctccggtaca tcctgggcga
     7921 tgagaaagcc tttgaagtcg ccgatcttca gaaatcagcc tttaatccca atgccttcga
     7981 atttctcgaa gacgaactgc attacgtcat cgccgtggtg tacgacaaaa aagaaagcac
     8041 cctttcaaac atcaaaattt ccatctccga cttcaaccag aaatattttc aaaacgacga
     8101 cctgaggata tccaatatct tccttgacca ggaagcgacc atccccatca tcatgatccg
     8161 gaaattcgac agcgccaccc aggccctccg ttatttcaac accgtcaagt ccaacgccaa
     8221 tcagtttatt tcagaccaga tccgccacga agtttttccc atctcccagt ccaattaccg
     8281 gaaattgtac gccaccaaag acatcgagtc gtacaaggaa ttttttaata gtaattataa
     8341 gaaataggcg gaattcaatt cttccgatcc atattattca cggagggtat gaagtgcacg
     8401 gagaccaaac tttctaccat ttatcatata aaataatctc cgtgttctcc tttgctccgt
     8461 gcgaaccaga acagaatcgg gggagactct gtgagcctga ctacagatac tgactcctga
     8521 ttactgacca ctaaactcat actccctcag actttcctcc ttcatctcaa tactgcaacc
     8581 cggcctggcg ggcaagcgat aagctccctg ccggattacg acggggtcga taaagtgttc
     8641 gtgaagatgg tcgacgtatt ccaggatccg gttttcgatc tggcctgaga tacccataaa
     8701 atcaaacatg gacaggtgtt gcacgtattc gcaaaggcct accccgcctg catgaggaca
     8761 gaccggaatg ccgtgttttg cggccaggat catgaccatc agggtttcgt ttatcccggc
     8821 cagccggcac acgtcgacct ggcagaaatc catagccccg ccggcgagaa actgtttaaa
     8881 catgacgcgg ttgtgcacat gctctcccgt agccacccca atagggaaaa tcgcatcctt
     8941 gatcttttta tgccccgcga tatcatccgg gctggtcggt tcttcaatcc accagggatc
     9001 gaaacgggac agggctttca tgcaccggat cgcctcatcc acttcccatt tctgattggc
     9061 atccatcatc agtttcatat cgggcccggt ttcttcacgg atgatggccg cccggtgctg
     9121 atcattggag ggatcggaac cgactttcat cttaaggtat ttccagcctt ccgctacggc
     9181 ttcccggcaa agccgcctga gtttttcatc ggaatatccc agccagccgg cggaagtggt
     9241 ataggcagga aatccgtttt cctccaggta ggccagcctt ttttcctttc cttcctgagc
     9301 cttgatcagg atcgccaacg cttcttccgg gctgatctca tccgtcagat aggtaaaatc
     9361 caggcaggaa aggacttgtt ccggcgtcag gtcatataaa agtttccaaa ggggtttgtt
     9421 ttccgatttc gcgtagaggt cccaggtggc attgaccagg gcggcggccg ccaggtggat
     9481 cactcccttt tccgggccga gccagcgtaa ctggctgtcc ccgcgcgtaa gggaagccca
     9541 gaaggcactg taatttgacg taatggattc gagcgtcctt cccttcacca ggtaggacag
     9601 ggaccggatg gcggtgacgc agatttcatt gcccctgccc gtagtaaaag tcaagccatg
     9661 accttcaagt ccttcccggt tggtttttaa tataacgtag gctgcggaat aatccggatc
     9721 ggtgttgacc gcatcggacc cgtccaggtc tttgctggtg ggaaagcgga tgtctaaggc
     9781 caggacttca gtgatggtga tcatgtttta ggctttatca ttaaatcgcg ggcggctgat
     9841 tttgaaacaa gccggcggat gccgcatcca tcgacttaaa aataaggaat acgggcaggt
     9901 ttacagcgga tagcggcacc cttattttat tttcggatcg acttgcttta cataccggtt
     9961 tagaaaggag accagttgaa aggcaatgat ccggtggtca tccacgctgg gatgaaagtc
    10021 acaacctccg ggcttcatgg gttcaaactg aaataccacg gcttctttgc gatccggata
    10081 ggcctggttt acctggtcgg ctatttttag gagcaagcgg tacaataagt ccgccttttc
    10141 tccggacaac atcgggctgg tcaacaaaac caccctggcg gaatcatagt ggccgtatag
    10201 ggttttgaca aaatccaggt aggtctccgt aaaccgggtc gaatcaaaag gttcccgggg
    10261 ggtgacgccg tctccattgg aaaaatcatt ggtgcccagg catatgctta cataatccgg
    10321 ggactcccta ctaaaatccc aggctgccga acgctctttc gaaaaataag tggagccgta
    10381 cagttcaggc atcgcgggtc catcgctgtt ccagttacgg taaatgccaa cccccgaaac
    10441 ggcactgatc gtgtaccgca tctttaattc gcgggccacc cggctggcat aggaataata
    10501 agcattgtgc tggtcatacc acttgccttc cccacaggcg acctctgagg tatcggcgcc
    10561 catcccgcag gtgatggaat ttccgatgaa ctctattttc agtttggata aggattcgac
    10621 cggtttaata tcccggcccc tgatgcggac tacggccagg tcgccgttct gggcctcggt
    10681 ggccttatat attttcagga cgtggacggg tgctttgatc tcaggataaa cggtgaggac
    10741 ttgttctttc ctgtcggtta tttttacccg aaccaccttt ttgacatcga gttcataaac
    10801 aatataaccg tagtcatcgc ccggggccag gtttttaaaa aacacctgga cactgtcgcc
    10861 cgtaaatgaa aactgtaccg tcgacatgct gctgatcagc accaggccct gcccttcctc
    10921 atataccgtc ctccccagga ccgtcacggt tttggaattg accggatagt cccggtgaac
    10981 aggtttacat tgctggaata atagaaaaag acccgccagt accggtaaaa aaggcttgtt
    11041 gagaatgtcg aatttcatgc acgtcaatta aaaccgcaaa gattggaatt tcggatcaga
    11101 ccattcctta gaattggatt aacgggtgaa accataaaac ccataaaatc gaatccacag
    11161 ggattcccat tccctgttaa tccgttttct tctgctccat ttccagttcg tccatctgtt
    11221 tgtacaaggc gcggaaagtg tcccaagggc tcctgttgtt gcgggtggcg ctgttattta
    11281 tattattggc cgaagtttgg atcccgtaaa acccgtcgat gaactggctt ggccctgacc
    11341 ataccgtttg caccatgccg taatacctgg gcttcatttg attggtggca tattttctcc
    11401 aacgggccat gtcttcggcc tgcatcaccg ccacctgctg gcggttccag gggcaggtca
    11461 ggacccggaa tcccttcatg gcaaaataaa cggccgtttg atccggccgc tcataatgcc
    11521 agtcgcagat caccacatcc ttgtagatca tgtcaatggc gcggtgggta tagttcaaac
    11581 tggcttccca caagccgatc cccgtgctct tgccgtcaat caggcggtcg ccccagatcc
    11641 acagttcccg gtttttgagt cggaggtggt tgcggatctt gtttacctcc ccggcaaaaa
    11701 gctccgcttt gtcgatgccg ccgcagcgcg ggcacttgtc gtccccgata taaaacactt
    11761 catccatgcc ggcgtggaag gcatcggcct gaaagcggtc acagagttca tcgaccaggg
    11821 cgaagaccac tccatgcact tcagggtgga gggggcaata acttttgcaa tacaatccgt
    11881 ccgggttggg ccatttataa gtctccggca ttttcacatg cggggtctcg tcaaactggg
    11941 gatagacggc cagcaattta ttggtccggg aagcccagga ctggtgtcca agcaggttga
    12001 tctgaggtac gatccggata ttgtttttcc tgcaggtggc gacgatcctg tcgacgtccg
    12061 caccggacaa ggcgatggaa tccctcagtt ccggatggga ttcgtattgg taattaaaat
    12121 ccacgcgcag gatcagggta ttcactttcc tgggagccat ctcctcatcc acaaatttta
    12181 aaaagcggtc cagttccgca ggtttgggag cgccgatggc aaaacccctg acttcaaaaa
    12241 gcgtgtccag tgcctggact tgggtcagac cgcaaagaaa aagacatgcg gcgatgatcc
    12301 ctgttttaat gcctgtttta ttcattccat tacgatttta cagtcccttg ggctttgtgt
    12361 gtcggattaa gaataggatg accaggaagt gaattgctgc cggaggcaat ttcttttatt
    12421 tcgcggagcc gaacaagtcc ggccagttta tttctaaacc cgtaagcacg gaagacatgg
    12481 cttttaagtg ttcttccccg tccgccgatg aataggtttt gaatttattg ccttccaggt
    12541 gaaacacctg gatgaatttg ttttgaggat caatgatcca gtattcccgg actccaaatc
    12601 gctcataatc ctcctttttc tccacataat cccggatgat tgaggatttg gaaacaatct
    12661 cgaccaccaa gtctgggcaa ccaaagaccc cgcgttcccg gatgcggtct tcatgatcct
    12721 tggccacaaa aagcagatcc ggctggacca cattgttttc atccaggatg acgtcaaaag
    12781 gggcgagaat aaggctaccc ttcttgtcta tattgacaaa ttgataaaaa agctgaaata
    12841 attcctgaag caactgctga tgggtaaaac tgggtgagga catatcgatg agttcattgt
    12901 tcctgagttc atagcggtca tcccccttca gttccttgag cattcgggta taggtccatg
    12961 gtgcttgatg tacggtatcc ggcatcctgt cttttttaat tcaagttaat ggctttttac
    13021 cattcttagc cttaattcct ggcctcctta attcccttaa tttattaatt cttaattatc
    13081 ccaaaagggt gtttcttgca gcatccagca actgcctgac cttaaccttg tcggccgcct
    13141 gggcatatac ccgcaaaacg ggttcggtgc ccgaaggcct gaccatgatc cattcatcat
    13201 aaccgaaaag gaatttgtaa ccgtccatgt cttccacttc ctggacctgg taatcctcaa
    13261 aagcggtata agcccgctcc ttgcaggctt tgatgatggc ctgttttttc tcctcgctga
    13321 gatggaggtc gtcgcggtcg catttgaagg cgccgaccat ggaatagatt tcattgatca
    13381 gggcggtcag cgatttgccc gttttggcca tgaattcaag gatggtgagg gccacccata
    13441 ccccatcccg ctcggggata tggcccgcta cggcaatgcc gccggattct tcaccggcca
    13501 ccaggatggg ggtggtgagc atgatctcgc tgatgtattt aaacccgatc ttggtcacta
    13561 cgcattccac gccaaacagg tccgccagtt ttttgacctt atccgtaacc gaaaacgtaa
    13621 tcagcacttt accctgctgt tttttaaaat ggtgctggta atacatcagc aaaagcaata
    13681 aatggtggga gtctacaaac tggccgtgct cgtccatcat gccgatgcgg tccgcgtccc
    13741 cgtcattggc caccccgagt ttgatatcct tccgggtcga gacggtcctg gccagttcgg
    13801 tcagattccg gtcgatcggc tccggggcct gaccgtgaaa ggaaggatta aaatcggaat
    13861 gcaggtggat ggcttccgga aacaattttt tcatgatgcg ttgccccgcc ccgtacatgg
    13921 cgtcaaaggc aaagggcatg gcggtggcct ggatggccgg gatatcgaat gccgccctta
    13981 ctttttcctc ataaaggatt tccagatccg ttttttcaat ctttcccaaa gcagcctttt
    14041 ttgccgggac ttcatccggg atcagggcct cgatttcggc gatctctccc gggatcatcg
    14101 gccccccata ggacgccttg agtttatatc cgttgtagga cggagggttg tggctggcgg
    14161 tgatcacgac tcccaggtcg gcttttaatt ggaggacggc cagggacacc atgggcgttg
    14221 aaacataatc atgggaaagg atgacctcca tcccttcccc gctgagcact tccgccgtgg
    14281 cctgtgcaaa aagcggtccg ccaaaccggg tgtcataacc caccaccgct tttgacatgc
    14341 ccttttgttt catccaggcc gctgtcccaa aagcaacccg ccttacattg tcgacggtat
    14401 attcgtccgc gatgatggct ctccagccat cggttccaaa tttgattttc aacatagatc
    14461 ccgtatttat ggacgcaaga taaaaaagat gcttgagtcc cttcctccct tccttggcta
    14521 ctgacgctcc cttcaacttt cgcccttcac ccttcacctt tcgcccttcc tcctccctcg
    14581 actattgacg ctcccttcgc cttttaccct tcgcctttcg cccttctcct gcctcggcta
    14641 cttacgctcc tttcaccctt cacccttcgc ccttaacctt tatattgcta tttttacatc
    14701 caatagaaaa ctactccttg aacaaaaaaa ataccacagc caccatcgcc cccgatcacg
    14761 gccgcctgga agacaatcac tggcaccggg gcctccgcaa aaaaatggtg gaagacctcc
    14821 ggcacaaggg gataaaggac gagaaggtcc tccaggccat gctccgggtg ccccggcatt
    14881 tttttgtgcc caagggattt gagtcctggg cttaccggga tgtgcccttc cccattggga
    14941 gcgaccagac catcagccag ccttatacgg tggcttttca aacggcctta ttggatatcc
    15001 gccccgggga gcgggtcctg gaaatcggga ccggcagcgg ctaccaggcc gcggttttgt
    15061 ttgaactcgg tgcgaaagtc tacaccctgg agaggcagga aaaattatac caccagaccg
    15121 cctccttttt gaaaaaaatg caatacctct cggtccgctg ttacctgcgc gacggttttg
    15181 aaggcctgcc caaatacgcg ccttacgaca agatcctggt cacctgcggc gcgccggaat
    15241 tgccccaaaa tctcctggac cagctcactc ccggcggatg cctggtgatc cccatgggtg
    15301 acggcgagga ccaggtgatg aaaaagatta ccaaaaaatc ggacggcgaa tgggaggagg
    15361 aagcgtatgg ggtcttccgt tttgtcccct ttttaaaggg cctgaacaag ggttgactcc
    15421 gcgataatca agaatactgc agaaatgaaa tatgcattga ttttaattgc tctttgtttt
    15481 accgccggtc tatcgggtca atccaaccat ccccttgccc ttttcaacca atccatcgat
    15541 gacgccgtag tgaaaaaaga taccctcaac ctgaaaaagt ggtatgcaga ggattttatt
    15601 ttcacccacg gtacgggcct ggttgagggc aaatccagct ggctgcgcgc cgtagccaac
    15661 ccaaactccc gcttcatttc ccgccagcac gattccgtca cggtggaact tcaccataaa
    15721 aacatcggga tcgtttacgg caaactgacc gtcacccggc ataaccggga aaaccaggtc
    15781 cactatgcgc tctggtacgt ccgcgtattt cataaaatga aaaaacaatg gcagatggtt
    15841 tcccaccgga cggtgcagga aagccattta tagatgtcga attgcggaat tgtccaattg
    15901 ctgaattgtt ttagatgtac ttccctcaaa ttgttctccg agcccttcga ttcgctgtgt
    15961 ccttgtggga aacatttgcg ggaaagccgc gtcgcggcac atttcccaca ataaacacaa
    16021 agttggctcc cacaacgaca caaagaacac gaagaaaaat acaatggata accaatttct
    16081 ctgtggcttt gtgtccttgt gggaaatatt tgcgggaaag ccgcatagcg gcacatttcc
    16141 cacaataaac acaaagttgg ctcccacaac gacacagcga agcgaagacc acgaagaaac
    16201 aagcgatagg ataacctatc cctccgtgcc ccttgtgcct ttgtgggaaa tatttgtggg
    16261 aaagccgcgt agcggcacat ttcccacaat acacacaaag ttggttccca caacgacaca
    16321 aagaacacga agaaaaatac aatggataac caatttctct gtggctttgt gtccttgtgg
    16381 gaaatatttg cgggaaagcc gcatagcggc acatttccca caataaacac aaagttggct
    16441 cccacaacga cacaaagaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 ccgcatagcg gcacatttcc cacaataaac acaaagttgg ctcccacaac gacacgaagt
    16621 ggatgcaacg gtcaaaggtc aaaggagaaa aattacaggg agatctttct attaataaat
    16681 cctccgtgct cccagtgccc tccgtgcgca acaccccaca acgacttgac gaccacaatg
    16741 aaatatgcca tgggatgacc tatctctccg tgcccttcgc ttcgctgtgt ccttgtggga
    16801 aatattcgcg aaaaagccgc gtagcggcgc ccacgacgat tcgaatgaga ggtaagggtt
    16861 aaaggtcaaa ggagaaagga gaaaaattac aaggagatcc tccttttaaa aaatcctccg
    16921 tgccctttgt gccctccgtg cgcaacaaac cctgtgcgtg cagtgtatcc cgcgaatgcg
    16981 ggagataacg gcagggtcaa aggacaaaaa attacaaaga aatcctccca ttaaaaaatc
    17041 ctccgtgccc cccgtgccct ccgtgcgcaa caccccccgt gtgaacccac ccggccctaa
    17101 ctctgcacaa aattcctcct atccagtttc atctccccat tccaccggta agccgtcacc
    17161 acctcattct tcaccacccc ataatccagg cagcagatat ttttcttcaa cagattgggt
    17221 ttgccgttga gggaataatg cccgaaaaag accggcaccg ccagttcggg ataggcaagg
    17281 tcgaggctga tcaggtttga agggatctta tatttcggca gattggattt ggcctgggtg
    17341 gagatctggc ggtaggattt gccgtggtgg tcctcccacc attttacccg cgtaaagccg
    17401 cggcggacgg attccgcatc caggcctttt ttgttccggg gcagggggat ctcgaggcct
    17461 ttgagcagca tctgcactgc cttgtattca gggccgcggg actgcaggga ctggatcaac
    17521 aggtcccggt tgaatttatg tccaggaaaa tgtttgcgca catagttaat gctgtcctga
    17581 tgccagcagg catggaccac gcgcaagtcc tcaaattcca gaaacagcgg cagggtgtac
    17641 atccagtcga tgtagtcata aagttccgct tcggcgctcc ggaaactctc aaaaaccggc
    17701 agcagctgga gcatgttgcg gatgctgtgg ctgtaaaggg gcttattgtt attgtccagg
    17761 gtaaaaaagg ccatcaggtt gagttcatgg ttgcccagga tggcgtaggc gtgtttggat
    17821 tggaccatgg gcctcacgat ctgcaggact tccctggatt ccggcccccg gttgaccagg
    17881 tcgccgacga aaatagcctg gcgtccgggg tgttgataaa gtcctttttg cggtttataa
    17941 tccaggagct ccaggaggcg gatcaggccc cgggcatggc cgtggacatc gccgatgagg
    18001 tcatacataa tttcaattcc ggaatcaaag ataattcata ataagaagct tccatacgat
    18061 ccttcgcaca tcatacgcca agatccttca ttccatatcc aagatggctg aggttacctt
    18121 tctcctcttc catcattagg aatattgaaa ttctaaatga ctttagttat gaaaaccaaa
    18181 accctttacc tgagcatctt acccctttta tcattattgc tttttggggc ccgcaaatca
    18241 aataccgtct accaagccga tgaattccac cacttgttgc ttgaggaagt ttcctgtcat
    18301 ccagattacg atgccctcgt ggatttatat tattctacaa acggtccaaa ctggaacctc
    18361 aaccctggct ggctggccaa ttgtgacgtt tgtatttggc ctggcataac ctgtgctggg
    18421 ggtcgggtaa ctgaagtaag gcttgtaagt aaaaatttaa acggaccgct tcctgcatcc
    18481 attggaaact tgtcatccct tacctacctg gatttggcca acaattccat caatggttct
    18541 atacccaatt ctatcggaaa cttgacgaat cttgagtatt taaggtttga caacaactcc
    18601 ctgtccggta gccttcctcc atcaataacg aatctgattc agttgaaaca tttaaagatg
    18661 gagcaaaata caattaccgg gcagatccct caagatatcg ggaatctgat ccacctggaa
    18721 agcctggatt ttaataattg cgatctgtct ggatctttac cggaatccat tggaaactta
    18781 atatatctca agtccttgct tttgtcttac aatcaactgt cgggcccttt accaacttca
    18841 atcggtcagc tttccgatat tcagagcctc atgctttctg gcaatcaatt ttccggcata
    18901 ttgcccagaa ccctgggaaa ccttgagcaa ttaataaact tacatgttaa ccaaaatagg
    18961 ttcaccggct gttttcctcc acaactaaac aacctgtgtg gccagctgat cagctataat
    19021 ttttccggga acctgataaa taataataac aattttaatg cgttttgcag tacaaatgtg
    19081 ggagtatgtc cgtgtcagga tggctatccc gtcacctggt atcttgatgc agatggagat
    19141 ggacatgcat caataagtac tcaggcttgc gatacccctg ggattggtta cgttaatact
    19201 acgattccag tgaatgattg caacgacagc atttcatgga tccatcctgg agtgttagac
    19261 agcacctgca acggcattga cgagaattgt gacggatcgt atgatgaaag ctatacaaat
    19321 ccggaatgcc aaatatgcat caatggaatt ctaaacgatt ccaccaatac ctggtttctc
    19381 gatgcagacg gtgacggaca tgctatctta tctttgacga cctgcctctg gcctggtgaa
    19441 ggctatactg cggattcatt gcctttggat gattgcgacg atgtcaatgc cctggtttac
    19501 cccggggccc cggattccct atgcaatgga attgataaca attgcaatag cactgttgat
    19561 gagggttatg taggtgttaa ctgctcgctt tgtactaatg gagaggaatc cgccaatttg
    19621 gactatttta ctctggttga atttttttcg gccactaatg gggatgcctg gaccaacaat
    19681 tccggatggc tatccggctg taatatttgc gagtggtatg gagttacctg caatactact
    19741 ggcgaggtaa gccagctaaa cctggcctat aataatctga caggaatttt gccagcctca
    19801 ttgaccaaac tttcaaaact tggcaaatta aatttgatcg gtaatttact caccggaagt
    19861 attccgtcgg acttaggaaa tatcaggaac cttcgtgaag ttttcctgga caacaactcc
    19921 ttaaccggat ccattcccgc cagcctgggt aatctgacct cccttcggtt tttatatctc
    19981 aatgacaatc aattgaccgg atccattccc gccgaacttg gaaatttatc aggactgagc
    20041 tcgatgattc ttcacaataa tcaactcacc ggaactatac cctccactct tggcagcagt
    20101 ctctccaacc tggattattt aaatctgagt cacaaccagc tttccggttc aattcctgcc
    20161 tccatcggtg aaattccgaa tatcctctcg attaaattaa atcacaatca gctttccgga
    20221 tccattcctg gaaatatagg ggattctcct aacctgaact atttgttcct taacaataat
    20281 gagttatccg gttgtcttcc ttcccaacta tctgtattat gcgaaaagct ggtggaagga
    20341 gatttttccg gaaatcctga gcttcccgga ggaggcaacc tggcagcctt ttgcatggat
    20401 agtaccggat cctgttgctc agccggaaca gaattaaatt ggtttctgga tgcagatggg
    20461 gatggccatg cctctgccct ggtccaggcc tgcagtgccc cagggatgta ctatacttcc
    20521 gattctatcc cggtagatga ctgcaacgat atgaatccgg ccatctttcc cggggcaccg
    20581 gattccacgt gcaatggcat cgacgaggat tgtgatggat cagccgatga tggatttatc
    20641 gtcgtatcat gccaaacctg cacaggtggt acaattcatt attcaggccc acctgccccc
    20701 atcgccagtg tgaccaaaca actcacctgc accatcccta cgggtactat taccgtatcc
    20761 aatcctacca cggggcatac ctatagcttt gacaatggct tgagttttca aacttcccgc
    20821 ataaaaagcg gtttggattc aggcctatat tttgtaaaaa tcaaatcaaa cctcaccgga
    20881 tgtatttcgg aatccatcca gcttatcata aatgggtttc cccccgatcc tcccgcccct
    20941 gaggtgattg tcagcgcgca acctacctgt gcgattccta ccggtaccct caaggtgacc
    21001 gataccctcc caggctttaa gtacagcttt gaccagggtt tgaccttcca gacttcaaaa
    21061 accaaatccg ggcttactcc cggatcccat caggtcttcg cccaatccat cgctaccgga
    21121 tgcatctccg ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaggaac
    21241 catcacggtt tccaatccca ccaccgggca tacctatagc tttgacaacg gcttgagttt
    21301 tcagacatcc agagtgaaag cggacttgca accgggttgg gttcagatca ttgtaaaatc
    21361 caatgccacc gggtgtatct ccgattttac tgtagtgcat gttgatacca ttccggaaaa
    21421 ccccgctcct ccccaggtca tcgtcagtgc ccagcctacc tgcactaccc ccaccggtac
    21481 actcaaagta accgataccc tcaccggctt taaatacagt tttgacaacg gactcagctt
    21541 tcaaacttcg aaaaccaaat caggactcgc acccggatcc catcaggtct tcgcccaatc
    21601 catcgctacc ggatgcatct ccgctcccac cctggttttc attgactcca tacccaccag
    21661 cggcccgcct gctcccattg ccagcgtgac taaaccaccc acctgcaaca ccccgacagg
    21721 aaccatcacg gtttccaatc ccaccaccgg gcatacctat agctttgaca acggcttgag
    21781 ttttcagaca tccagagtaa aaagcggcct ggattcaggt gattatcatg tcagggtcaa
    21841 atctaatctt accggatgca tttcggaagc gacctttatt tacatcggcg gactccctga
    21901 taatcctccg gctcctcaag tgatggtcag tgcccagccc acctgtgaca tacccacggg
    21961 gacggtgaaa gtaaccgata ccctgcctgg ctttaaatat agctttgacc agggattgac
    22021 cttccagtct tcaaaaacaa aatccgggct tgctcccgga tatcatcagg tcctggcaca
    22081 atccatcaca acgggttgtg tttccacacc cactccggtt ttcattgacg ctgtcccaac
    22141 cagtggacct ccacccccca ttggcggcgt gaccgtgcaa cccacctgtc cttctcctac
    22201 cggaaccata accattttct ttccgaccag cgggcatacc tatagttttg acgatgggat
    22261 gagtttccag acatcccgga taaagaatgg ggtgacgtcc ggtacttatc tgctgcgggt
    22321 taaatcgaac gccaccggct gcctttctgg tcccatttcc gtgacggtaa atgaattgcc
    22381 atttgtaaaa gctcccgatg cttcggttac agtgcaagct acttgtgatt ccccttcagg
    22441 tacaattgtg gtcagtaaac ccacaaccgg ccatacttat agttttgacg atggccaaag
    22501 ttttcaggat tccggaaccc tatccggcct gccgccttcc atatattatg tccgggttaa
    22561 gtccaacact accggttgta tatcggaggg aagaattgtg attatcgaca gcgtgttctg
    22621 tgattctttg atttcaggaa ttacctttcc ttccaggata tacagcttga gccccacatt
    22681 tcaaaacggt agccttccca aatcccacac cggatcattt aaactctacc ctaatcctgc
    22741 atccggttat ttcaggataa aaatggatca actcccatct caaggaaaga tcacggtcag
    22801 gatgacggat tcttatggcc gggtgctcag acagtttgag cgttccatta cggaattggg
    22861 aaacgaatca ttccagacac aagatttgcc caggggaatc atttacatcc aactgatttc
    22921 agaggaaatc cggtacccgg tcggaagact ggtgctggtg gattgatcta tacccttgag
    22981 catattcatt tcgattttaa ccctgcacga aagtgcgggg ttttttataa tgttatacat
    23041 gaaacaatga aataccacaa atccataaaa tcccacaagg gcttacaaat ctcctaagaa
    23101 tctcctcctt cccaaaacac ttcatgttct tcgtgccttc atgggcaacc ctttgcgtct
    23161 tcgcaccttc acgagaaacc atcccctgtg actatgtgca tcaagcttcg cgtcttcgcg
    23221 ccttcgcgag aaaccatcca ccttgcgcct tcgcgagaaa ccatctcctg tgcctatgta
    23281 catcaagctt cgtgttcttc gcgccttcgt gggaaaccat ccacttcgcg ccttcgcgag
    23341 aaaccatctc ctgtgcctgt gtacatcaag cttcgtgttc ttcgcgcctt cgcgagaaac
    23401 catccacttc gcgccttcgc gagaaaccat ctcctgtgcc tatgtacatc aagcttcgcg
    23461 tcttcgcgcc ttcgcgagaa accatccact tcgcgtcttc atcccttcgc gagaaaccat
    23521 ctccttttgc ctgtgtcatc aagcttcgtg ttctttgcgc cttcgtggga aaccatcccc
    23581 tgttggctcc gtgagaataa aacaataatt ggttaaatac ccactacccc gaatattttc
    23641 tacccctcat gtttggtagt ctgaatttcc ttttatatat tcatcccaga caccaattta
    23701 tgcaccggga taccacccat atcatagacc ggattcgcag tggagatgag gatagcctcc
    23761 gtgagatata ccgcgaatac cgccagcctt tcatccagtg gatcaagggc gaatttttct
    23821 gttcagatga tgatgccatc gaatttttcc agactgccgt agtcatcctc tatgacaata
    23881 tcatcaccgg caaactcacc gaactcaaca gccatgtcaa aacctatctc ttcgggatcg
    23941 gcaaaaacaa ggcccacgaa tggttgcggc accggcaaag gcagggggtt cattccgacg
    24001 aactcctgat caactaccta catgaagaaa accccgaagt ggatgaagcc gactttgcag
    24061 aatcgctccg ggccgtggat gccggcatga atcaattggg cgagccttgc cgcagcctgc
    24121 tcagctattt ttattaccag aaactcagca tgcgcgagat ctgtgaacgc ctcgactaca
    24181 aaaacgaaga cacggccaag aaccagaaat acaaatgcct caagcggtta caatccattt
    24241 gtgcggacat taagggggta aaagtatgac gaacaccccc gaacatatca gagaaaccct
    24301 ggaactctat gaccgcggag agctcgaccc ttcccgtttg cttgaagtcc aggccttatt
    24361 acgctcagac ccggcctggc aggcagcctg gcgtagcttc cgctcggaaa ccgcggggat
    24421 caggctgaac aggctcaaaa caaacttgtc cgtcctggaa tccctggagg ccgaactaaa
    24481 agatccggaa aaaaaaacgg cggccggggg tgaccccgga gctgttttga catcaagtcc
    24541 tgaatcaaac catataaaac aggcatcgga aacgtcccag cctgaaagca tggaagaaac
    24601 catcgatcgg aatacctctg aaggcatccg tttttccagg ctgcatcagg ccttgaacgg
    24661 cctcaaagcg gaagaaaaaa aaactgccga cccacgtccg gctggcggcc gcatcctggt
    24721 tattcgcagg atctggctgg gggctattgc tgcgggcatt atagggtttt ttcttgtaat
    24781 gcaattattc aataacctga atcgtcctaa atatataaac caatatttaa ttacacactt
    24841 tgacgaatat attttacatg aaaaatcaag aagcaaatcc gagaaattaa cttttaacaa
    24901 agataaagaa attggttacg atctttttgt attgcaagaa tttacagaag cattacctta
    24961 tctacaaaaa agctggttaa actcaaacga tactttatcc tttttttatt taactgtcag
    25021 tcatgcagcc cttggtaata ataaatattt tcgcgaatac aagtcaagtc ctgtcttaca
    25081 atctcaattg tatcaaattt tattaaacaa aataaagtga tatgaaactt ctattaatct
    25141 caactatatc tttcatgatt ctatattcct gcgaatttga gggaaagaat cacactcttt
    25201 taccaactca gttattagaa actattactt ttgacacagt aataagtttt aatccattca
    25261 tgagcatagc ccaaatcaaa acagatacct tctttttttt taataacgat tccattgaag
    25321 ctcataaacc gtattccaat attgttcaat acgactttag agctcctaga aattgggact
    25381 ttagtcaacc tcaatcggta ttatataaat tcgtctcaac agattatcaa ctttctggtc
    25441 aaggtttaat gccgttaggt caccatggaa atctttcatt gaatggagtg gaaatattgg
    25501 cccaaaaggg ccgcttatgc tctcatagaa acgcatcatt ttacaatggt actttatatg
    25561 ctattaacaa ggattcatta tattttatcc catttgatgg catatatgat cacgaatgga
    25621 attgcttatt ttctcatcat ggcctgcaaa aaaattacat tcatggcttt tttataagac
    25681 ttactaagtc cgaattttat tctgcaacac aaacaggata tgtgcagcat aatataaact
    25741 gtgatggtat aaaccatatc agatgtataa acttcaaatc ctatcataca aaaattggaa
    25801 ccacactttg tcctagaaaa gcaatcgaac aaggaaacaa taattatttt gtttacttct
    25861 ttagtgatat taaaaactaa aaacttactc caaagcaaat tgttttccta tataaatacc
    25921 acgaactcaa attcattttt aacatcatta atttttttta acgatgctat aattctacga
    25981 gctccaataa ttttaaaata tctcaccata ttatatgtcc cttcataatc aattatgtcc
    26041 ccaattcctg tttcacaacc tgcgagtgta atctgaaatg ttttagatct atattttagc
    26101 aaatcatatc caaatagagt atcaaattct gaaaatgtat tggttctaaa tattagatat
    26161 acattttccc tttcattcga aattacaaca ccatggtgtg caatatacaa atggttatat
    26221 tttccattcg ttaaacaatc aataaacctt gctcttgtgg attttaaacc cgaataactt
    26281 atattgttcg cctttttttt aaactgaaga atctcatgat atcctcctgg aagctccacg
    26341 aatgaggaag taggatttct aatggtatta gggtccgaaa acgaaaatgc agcagtcttt
    26401 aaatcatttc tttgaatcat accttcctgt ttcatggtgg aagctgaaaa agtgtaaaac
    26461 agattataat gctcctttaa ataatctttc ccgtctggaa ataataataa attaaaattt
    26521 aagttataaa attttcctga tgggcaaaat acaatactct tcttgtcatc taaatttagc
    26581 tttcctaaaa taactttata cactatattg gacatcctta tatattctaa atgatcgtat
    26641 ttccccaatc gctgatactc tattaatgaa tccagcgttt gaagaaaacg gtaatccact
    26701 ttttctttaa atatctgatt tacagtacta atatttaata tatacagatt tgaatcaatt
    26761 aaatatatgg ataaaaacaa tactgtatct ggaatagcaa cttcttttaa tgattttacc
    26821 tctcctactg tcttcgcatt aaaatattca ataaaattca tttttagata tttattaaac
    26881 tcatcatact ggaaatacaa ttcgtccagc ttcgcctctt tcttcttccc catgcggcag
    26941 gctcgaattt cattttttag gaattcttct tttctaacaa catcctcagg aatcattaat
    27001 tttaatttaa cgtcattcat ttcaccactt aaaatgctcg ctttaacttt ctccatgcat
    27061 ctataaaact tttcaaatgc ccaaacattt tcattacttt tccacattac aaaatatatt
    27121 tccattgcca tttcatatat acttttactg tatgacaata cctccaaccg acgggtttct
    27181 tcaatgctat agttttcttt gttgatcatg tcttctgctt tatccaataa tcgaatggcc
    27241 atgtttaatt tattctcatt tccacttaat ttaaattcca aaaacaagtt ttttattatg
    27301 tcaccaacga ttataaaatt aaaataacca taatccgcca tccccatcag aatatcttca
    27361 acagtaaatg ctttaattgg acttttataa ttgatattat taaatgcttc cgccgtggat
    27421 tcacttaatt gaccgattct acggtaactt tcaataagaa gtgtgcttaa cgtgaggtac
    27481 acagagggag ggaaccgctt aagtgaaaaa agatagagaa tagctttttg ggaatgattt
    27541 atggtattta aatacttacc attcgaataa aacgcttctg cactgatctt atccacattg
    27601 acaaaagtgt cacttgaagc aacgattttc attaaatcat tgttcaataa ttcagccaaa
    27661 cgctcattgc cacgattaat gcaagaatag gaaaatgaat acaaaacttg ctggtaaagt
    27721 ttgatgtatt tagtaccttt aaccattaaa agcgcttcgt tataatcctt aatgtatccg
    27781 gaatcccggt ccacaattgc ccgtatatat ccggaattat aaaaagcata ggattgtagg
    27841 attgtatctt ttgtaaaata tatactcaaa tccttcattt cctctcccag ttggccccca
    27901 aacaggtatt gacgttgccc gatggttaaa agaataagtt ctttttttgt gtaaactgtt
    27961 tcatgcgtaa taaagggtaa ggaatcaaat ttggaattag cctttatata atatgcataa
    28021 gaaccataca ggtcattcag aacataaaag gtatattctc cgcgaagtag gtccatcttt
    28081 gctctccaat aatcaggaac cttttcattc aatagaatgc tgtcacacaa agttgtaaaa
    28141 actccgaatt cgatttcatt gtacaggtta atttcatcca taaaatataa cagcaaatcc
    28201 tttgttgttg aaaactcact taactgaatg ggagtatggt caaataattc agtggtaaga
    28261 accatatggg cattgagcct ttgcgctgcc tcattacgtc tgaaatagag ataattttta
    28321 agactttctg atgatttaga aatcaagtca tcatatcgtt caatagcttt ttctaacaag
    28381 tgaccataaa acaaagaatc agccgaagct atttccacaa gcatggattt ttctatgttg
    28441 ggttcaataa attcaatgat ctcataaaat gaattggtct gtgaagagtc aatacctttt
    28501 gtataacaac atatccagct agtgcaaaaa cttatgcata acatccaata tttaagcaac
    28561 gttaacacca tattttccgg ttatttagca atatattcaa gtagacgtgg gtcatggcca
    28621 gcatactata ataaagctcg acgtcttttt tattgaccag gtccgcgggt tggatccgct
    28681 caaagggctt tccgcataca tggctcaccg cttcccggta cagcgggatg gccgaatccg
    28741 gttttcccgt aaggtcataa tactcaccca gccagtaggt ggaactcgga cagagctgat
    28801 aatcgtacct gttgctgcaa taggtccgca cccaggccag gtagggccgg gcctcctcct
    28861 gcctttgttg atgcatcatg tgaaaggcca tcgtacgctg ataataaaaa ctgtcttctg
    28921 gagtctggac catcggaagg gcccggtcca ggaaagtatc cgccttttca aactggtaat
    28981 tcctggaaag ctggatggca aaattgatca ggtcatgggc tttcaggcgg tttttggaaa
    29041 gattgtaccg gatatgttct tcaaaatagc ggttggcctc attggggtcg tccatatcag
    29101 tatagatatt ggccaatacc ttggatacgg agatcaggta attggaatcg atcacgggct
    29161 ccccgttgag tttttcgcga aggatcagcg catgggccaa tgcatcatcg aatttgccga
    29221 ggacccggtg ggtggtggcc agggtataga gctggttacg gtgaaatccg gtaaaacccg
    29281 gccggccggc caggatggta tccaatacct gtaatatggc catggccttt cccgggttgt
    29341 tggcatcgta gcgccacatc tcggcatgtt tttcccggat gcggagcatc agatcgtatt
    29401 gtggcagaaa aatccgctcg ccctccccgg tgattgcttt gaacaggcgg tctgaatttt
    29461 caaattgtcc tttgattcct atccgcacag caaacaaaag cttccattcg atttgcagtt
    29521 caggcaggat ccccagacta tcccctgcca gccttgcata caaagaatcg aaaagggaat
    29581 aattccggtt gtttaatttc ctttccaggt atttaatgtc cagataacgc gtattatttc
    29641 tctccttgaa tatttcatcc ttacctaaac ccgcataaag gctgtcttct ttacccctgt
    29701 gcaagcggaa aaggctatcg gcaatgaatc gtaccgaaga ccgatatgcc gcgtccgtat
    29761 ccaggatata ctggtttata tcgatgccgg tcttatctgt ccggcaggct gccaataaaa
    29821 acccccaaag gaagaggagg gcggtgcatt tcagtgtatt tcccaagagt ggcattttga
    29881 ttctgtaaat taagtgaaaa gaggaaagca tccaaaatag atgttccagg cccaccacaa
    29941 acaatccggt ggggataaac tatgcacaag cggtaatgac taatagcagc ctttaagatt
    30001 accggctccc cgcttcttta attcttaatt cattaattct taattcccaa gttgggttac
    30061 ctcctgaccc aaattacaca ataagaaaac caacctatta agcacacaag ggaattcaat
    30121 aaaatatgga taattctatg gctaataagc aaaaaatcat cgatgccttc cattcacgtc
    30181 caaatgataa gtggataagc ataaatgaac tcgcctcctt aacaggtatt gatcagaatc
    30241 aggtatcagg gatactgagg tcttcacctg actttgtgca atcgacccga tggataaaaa
    30301 acggagagcc ggtatatgcc accaaagaat cattcaggtc cggtaccgca ttcatcgaca
    30361 aggtgctggg tgcattcaaa aacagaatag attaaaattt ataaaatgat agccaatttg
    30421 atcaatgaaa acatcctttt accggcaatg ggcgggtttg cgatccagtt gctgtctctg
    30481 ctggaacttg caaaagttcc gattagccag cgacctgatc taaaatctct ggtgtactgg
    30541 attccttatc tcgtaaatcc attgctcggc ggattgttag ggtttaccta tttccacaat
    30601 gagcaaacca taaatcccgt attggccatt catatcgggg cctctgcccc gatcaccctg
    30661 agaatgatgg cttccagccg tcctcctaaa actatagaac ctcaaaggag tatccctgaa
    30721 aatccttaaa agagtaaggg cgagaacgcg gaaaatcgaa tagagtacgc actaaaatat
    30781 tttttataac ctaatttcta tgtaaaattt atcatttatt gaagacgatc agttcctttt
    30841 tcaattcgaa gaactgaatg cagacaattt caaagcagat caaatgttga cactaaacca
    30901 gtcacagaaa ctggtaagga tatgtgatct ctataaaatc atcaaaccaa tattattggt
    30961 tatccttagc attccctttg ttccaaggaa aataaaagaa gccattaaaa ttttcatcga
    31021 aaacctggac ctggtctgtc cgattccaac cgcttaatcg cacgtttccc gtctcatgat
    31081 acgcatatcc cgcctgtcct ccaaagatct ctgccatttc caaaccaggt accttgactt
    31141 gtccggccta tccatccccc ctggctatct tcgcgggcag acggtttatg gtttctttcg
    31201 gcgtgggcaa atgatcggag gattcatcct gggtaaagag cagccgcttc gcaccacgga
    31261 ggtcttttgt tcccctgatt ccagggaccg tctcaaccgg tccctggaat caaagggacg
    31321 ggtcctggag atctgttgtt tttggatcga acgttctttt cgcaaaatcc cctggataaa
    31381 tgcctggtgt tggcttagga tggcctgggc agccagtatg gagcgttcgt tcgatatcct
    31441 gttgtttggt actaattcct ccggcctggc caaaatgtat ggatatccaa aaaatgcttt
    31501 tcttcttcat agcgaccaaa tcgggggtcg cgacacctat attttttatg ccaagcgaac
    31561 gcattttctg gcaggcgtat tcgaaatagt cctagctaaa gttttccgaa aaacaagagg
    31621 attccgaagt tctactgacc gcggattgac ggccaaactt caaacgatga aagaaatgtg
    31681 cttctgaaaa tgaatcagga atcaggagca gggttacttt ttcaacctcg tctcattaca
    31741 atcatgaaaa atcaatccat ttctgtttta ttggtttacc tcctttttgg aataatcctc
    31801 ctcctttgcc tctatctcct ggccggtctg ggtttccggg tggaagcatt ggtttttgaa
    31861 aaatattaac ccaatatgaa tatggaatcc ctgattcttg acaagtcttg gaataaatta
    31921 aaggtttcca aaagaattca atcctcggat tatgaccctg tgttctggtc tcactatttt
    31981 catacctatg atgtcctgtt aaaagtaatt ccatatcgcc agctcctgca atccttagtg
    32041 gacgcagcat gcatccggcc cggccatatt attcttgatg ccggcagcgg caccggcaac
    32101 ctcgcctacc atctcccgca gggcgtgaaa accattttcg tggaccagtc cggggaagcg
    32161 ctggcccttg cgaataaaaa aaatcctgag gccctcacct taaaaacatc cctgacaaac
    32221 ccattgcctc ttgaaccgga ctccgtggac catatcatct gcaacaatgc cctgtatacc
    32281 ctgccctatg atcaatggcc tctggtcctg gatgaatttt accgggtgct ccggccgggc
    32341 gggtccctgg tcctttcaaa tatccgggaa ggcttttcgg tcttgcggat ctaccgggaa
    32401 catatccggg aaagtgtcag gctcaggggc tggacacata ccctggccga ggtccttcga
    32461 ctgttcattc caaccctgaa aatgttctgg tacaatgccc ggatcataag ggccgatgcc
    32521 gccggtacct ttcattttat tcacagggga gaacaaaact ccctgatgct cggtcatggc
    32581 ttctggccgg tcgggccaga acaggcggta tatgcgggac aggcctggat ggctgtgggg
    32641 attaaacgat attccaaatt ttagatattc caaattttaa aaaatttgga atatctcatt
    32701 tcttcaaggg ttactttacc cgtataggtg cataaaacat attaatcaat catttaaagg
    32761 taaaaccatg catcagtata cagtctcaga tccttcagcc ttagaaactt ctctattaga
    32821 attcctggct aaaagcaaaa cggatttaga gttggaaaat attaattgca tttttaattc
    32881 attcatccct cagttggcaa accattataa attattttgc caatctgaaa gatggaattc
    32941 ttttattaat atctgcaatc actcttcact tcgaaaaatt ttattggaag acccatatac
    33001 cagaagatct gttgaaaaac ccaggggata tgcaggggat gcctgtttaa ttgatttcat
    33061 gtacaaactt tctgctccca atccttctga tacatatttt ggcagagaac tcttttggca
    33121 tcttatggaa agcagtgcat gccaaagcgt ccgctggcgt gccctccacc tcgctgaaaa
    33181 aatagatgaa gtccaccagt caaatcaaaa acgaataaac atcctttccc tcgcctccgg
    33241 tcacctacgc gagctgcatt atgtccgtga ttttgatcaa aaagtagagc gcttctatgg
    33301 aatcgaccag gatccaagaa gcaatgaaga agcccgccgg tccctgccct acctacaatt
    33361 ggatatccgg gaggaatcaa ttttgaatgt gatcagggaa aaatacaaac caaaccgccc
    33421 gctagatttt atttattccg caggtctctt tgactatctc aacgataaag tggcccaaaa
    33481 gctcatcacc tcctccttca ggctcctggc tcctggtggc cgtatcctgg tcgctaattt
    33541 cgctcctgac cttgtagagc aagcttatat ggaagctttc atggactggt acctgatcta
    33601 ccgggatgaa aatcaaatgg aggcattggc ggaggatttg cctatttcac aagtcgggga
    33661 ggtaaagaca tacagggacg cgatgggaaa tgtagtttac cttcaaataa ttaaaaatta
    33721 aggaattaag aattaagggg gccttcattt tttaattttt aattttttaa ttcttatatc
    33781 gcggcctatt gatatcagcg cattaccgcc aggcctatat ttcatcatgg gaagcaagcc
    33841 gggctccgaa tttgaccctg ctaaatttat caaacattga tccactcctg tgatgtttaa
    33901 aacgatctgc atctgggttg catcgtgcct tcaggcacct aatttggata ggtcggattt
    33961 agaagctcat ctcgtcccgg atgggacgaa atatattccc tttcgaatgt gctacccata
    34021 taaaatccct gacgggatat ttggatctca acccttgaat cccttttttc agttgccctg
    34081 tatcaccttt tgaacaattt ccggggattt ggaatgatgc ccttttaggg cgatccgacc
    34141 tggccttccc gagcatcggg ctattcgccc gatgctcggt gaataatgcc ccttcagggc
    34201 aaactcggag atggggtttc caaattatca atgtctctat aggtgttagt tcggatggaa
    34261 cgacatatat tccttttcga atgggccacc catataaaat ccctgatggg atatttagat
    34321 ctcaaagcat gataaccatt tttcggtctg ccctgtatca cccttttgaa caatttctgg
    34381 ggatgatggg gaatgatgcc ctttcagggc gaattcgacc tggccttccc gagcatcggg
    34441 ctattcgccc gatgctcggt gaataatgcc ccttcagggc aaactcggag atggagtgct
    34501 caaattatca atgcctctat aggtgtaggg tcggagctaa gctcctcccg aacgtggggt
    34561 cggtggtaat ctggcggaat ctacccgcag ggatccaata tgggtaggtg atcctcctta
    34621 gatctcaaac cttgaatacc ctttttcagc cgccctgtat taccttttga acaatattcc
    34681 aagatgtcaa tatcccttct gtcggtttta ttcagatatt taaaccctgc aggtatggtc
    34741 agcccccatt aatgctccta tgcagtaaca tagacgatcg gtatatccca tgcatcgtgc
    34801 cttcaggcac ctaatgtggg tagatcggat ttagaaggtc atctcgtcca ggatggggcg
    34861 aaatatattc atcatcgaat gggctaccca tatcaaatcc ctgacgggac aaggtggtaa
    34921 tgcccaggca taccacccac ttcctcccgg aattagacca acctaattcg tccccgataa
    34981 aaaagaggat cttcttaatt catttaattt ctttaattct taattcccaa aaagggttac
    35041 ctcccccctc ctcctacatc caaatcagag aacagagtcc aagtcaccta aatccattat
    35101 ccataaatac ccatcattat gaaaaaaaca aatctaccct ttaccttatc cttcctgctt
    35161 ctgatcatgg gatcattcct ggtcagagcg cagtgtcccg acgatctttt cttcaacacc
    35221 caggaaaagg tggacagcct ggccattaaa tatcccaatt gtacggtatt gaacggggat
    35281 gtcaacattc agggaggagc aaatattact aaccttcagg gattcaaaaa catcaccgct
    35341 attgaagggt ccctcttcct ggcaggaaat tctgccttgg ccagcctggc gggtatggat
    35401 aacattactt ccatctccac ctggtttgcc gcatacggaa atggactaac cagttttaat
    35461 ggtttaaaca aacttaaaat catcggggcg agtattgata ttcataacaa cggatccctt
    35521 accagtttcg aggggtttga atccctcacc tccattggaa cctttgcggc ggtcaatcac
    35581 aataataatc ttgcaagttt tgagggccta agcgccctag tgagtattgg ccatgatttt
    35641 aatgtacacg attgtgctca attggctgac tttaccggtt tagaaaattt aaagtcgatc
    35701 gggggatatt tcggtgcaac ccgctgcggc accctgcgca atttccaggg cttaaacagc
    35761 ctggagaata tcggaaaata tgttgggatt gatgacaatg ctcaactgtc ggatctcacc
    35821 ggattggata acctcaaaaa cgtgggcgag agttttatac ttgtacgaaa cccaagtatt
    35881 aaaagccttt atggtctgag caaactgacg catgtcggac atgattttta ttttggctcc
    35941 aaccccgccc ttgaatccac agacggcctg gaaaatctga gtatgatcgg tagaaacctg
    36001 cattttgata ttcatgccgc tctgatcaaa ttgagcggct ttgaaaacct gacaaccatt
    36061 ggtggacatt ttgaattatg ggagaacaac aacctttccg atctgaacag cctgaatagc
    36121 ctcagatcaa tcggtggcga cctcagcctg gtcaggaata caaaactcgg ttctctcgaa
    36181 gggctggctt cccttgaaaa catcggtaga aacctggtca tcgacaacaa tatcggactg
    36241 accagcctca aaggcctggg caatttgaaa tccatcggtt caggtcctga tcccggggtt
    36301 tatatctata ataatgataa gctcagcgaa tgcgccatcg aagcgctctg caatgtcatt
    36361 tttacaaaaa accaatcgat ctacaacaac ggtggttccc agggcggttg ccggtcacag
    36421 gacgaaatcc ggcttgcctg cggccttgct gcaccctgcc ctgaaacagc tatgctgaag
    36481 acccagaagg aagtggatga atttgtccaa ctttacccca actgcaccga attaaaacaa
    36541 ctcgtcatag gcgatccatc cggcatcacc tccgatccga tcgtcaacct tcattccctg
    36601 cataagattc aacgcatcac ttccggccac cttgttattt ttaatacccg acttgtagat
    36661 cttaccggac tgaacaacct gaaatcggtt tcccgggcca taaatatcag cgcctccccc
    36721 ctgttgtcca gcctggaagg attttcctcc cttgaaagta ttggaggcgg actattccta
    36781 tccaaactgc aggctttctc cagcctgcag ggcctaaagc cgggcgtggt gaatcattat
    36841 tttcttcaga tccaggaatg ccctgccctg acgagtctcg aaagtttatc tgcaattctg
    36901 accggcaacc ggataagggg gctcttcctt ctgaacaacc cgggcctggt caatatcaat
    36961 ggactggaac agctcaccca tctccctggc cccctgacca tccgcgacaa tgccagtctg
    37021 gtcaatctct ccggcttaaa aaatctggtg agtaccgggg atctcatcac tattcagaac
    37081 aaccccgtat taaattattg ctgcgcgatc cagcccctcc tgaattccgt aaaatccgta
    37141 cccggaaaaa tcaatatctc caacaataaa tacggatgcc acagcctgga tgaaatcaac
    37201 aacgcctgca gcccctctcc ggccatgcct tatttcaaag cggtcctttt cgcaaccctg
    37261 accgcttccc ccaatcccac gaccggtctg gtgcgaatcc gggccagcat gcccaccctc
    37321 ctgcttgatg tggtcgatat gatgggcggc tcccagggta tcagccatcc cgcggccatg
    37381 gattttgaaa tcgacctcgg tgcaaaaccc gccggaatgt atgtattcaa attatttcag
    37441 ggagaccagt tgaagacctt gtccttgatc aaacaataac ccttcattct gttaatttct
    37501 ataattacct taattctctt aatttttaac agatatgcca gattttgaaa atctggcata
    37561 tctgtttatt cctccggctt caatttgagc gtcttaagca cttccggccc gctcagccgg
    37621 atctcatgac tcagattgag tttgtcccgg taaaacaaac tgagggtgat ttgctggccc
    37681 tggtaatggg taggcaataa gagttcaaac tggccttcgg gtccggtagt atcccgcgcc
    37741 acccccgcat tgatatcgag gatcaccccg gcggcggggc gggagtcaaa atgtaccacc
    37801 cttccggtcc aggactgcaa ttccggtttt ggttcagaaa gtactgagct gtttccttca
    37861 gggtttttac ctgctctgtt gaaaaggtaa atcacgccaa tcaataccgg gatcaacaac
    37921 aaccaccacc agggcgaata agatttattt tttacctcag ctgttagggt tccatcctgt
    37981 tttttcaatt tcccggcaga caaggcctcc tggctggtgg gcaccgaaaa gccttcattg
    38041 gcaatggcat agatatccat gggctcttcc acattcttaa agggaaagga gcctaccagc
    38101 accgttttaa actcaggctg gtttttaatt ttattataca cctccttgct gaacaggatt
    38161 gcgccggcca cgcccagggt ttcgatgcgg gaagcgatat tgatgccgtc gccgtggacc
    38221 ttgccgtttt cctcctccag gatatcggcg atgtggatgc ccacccggat attgacccgc
    38281 ggttctttcc ggagttcttc ctggatcgcc atggcgcatt tgatcacctg gatggcactc
    38341 ggcaggatgc tcatgctccc gtcgcccatg tcgtttttga cttcgcctcc aaaccgtttt
    38401 acacaggaat ccaccgcggc ctggtgtcgt ttttgcatct ggatggccag catttcatcc
    38461 cgctgcatga cggccgtata tcccaccatg tcggtgaaga gaatggcggc cagcttgcgg
    38521 gtatatcggt ccaggccctg caatggtttt aaattaatgt ataaaattat caatttccgg
    38581 ggatggggaa tgaagccctt tcagggcgat ccgacctgac cttcccgagc atcgggctat
    38641 tcgcctaatg ctcgggaaat gatgcccctt cagggcaaac tcggagatgg ggtaccctga
    38701 ttatcaatgt ttcaataggt gtaaggtcgg agcgaagctc ctccttaacg aagggggtat
    38761 ttataggtga aaaaatatta tctcaataag gattgccagg tcggagcgaa gctcctccca
    38821 aacattggca aaaatcatct cttgtattta accctttcat aatgctaaag agcttcgggc
    38881 gggacgcccg cctgaccaca cctttatctt attgatcagt cgggtaggca ctgaagcaac
    38941 cttgcctttg tttggctgct aacgtggagt ttaaagtttt ttggtcagcc ccaaatatgg
    39001 gcaatctatc ccgtcaggga ttcgatatgg gtaggcgcac cgtacaaggg acattcgtcc
    39061 cgtcagggat gaaatagccg gattgaatga gtggttacac ggtcggagct aagctcctcc
    39121 caaacattgt tgtttcaggc gtcctcgcct gaaacgtatt agttcttctc ttgtatttta
    39181 tgaacattat cgccacgacc cattttttaa cgtcttccca gcatcctttt ttgttaattt
    39241 gcccttcgat tcgtcatctt acccgcactg tttacattct tattcaatga aaccccgatt
    39301 cctgaccctt ctattgtatt tatggatcgc cgggccgctt tttgcccagc aaagcgtgac
    39361 tttaaggggc aaggtcgtgg aaatcactcc ggaggggacc gtcggcgtgc ccggagtggt
    39421 ggtcagcgtc agcggggaga gttacgatgt gaccgccggg gacggcagct ttaaattgtt
    39481 tgcacccggc ggactggacc agatcaccat caccatcaag ggatcttcct ccatgatgat
    39541 ctctccttat gaaggaaagg tcagcatccc cccgcttaat gaacccattg agatcaaact
    39601 ctgccaggaa aaaaacgaac agctgataaa taaaataacc cagctcaata cccggatccg
    39661 ctccctgcaa aagacgcaga aactgaccaa ccggcaactg gagcagatgc accggaccat
    39721 gctggacacc atcacctttt accaggcccg gctggacctg gcgaaccgtc agcttgaaaa
    39781 agccgaaacg gccaaccagg aattgaaaaa taaaatcacc gaactggaaa aaaccaacgc
    39841 cctactggaa gaaaaactct tcctggccct gggcgaaaaa ttcaccgccc agcaaaaaca
    39901 ttttgaatcc atcagcaccg gcctcaacga ctatatctcc cggctgaaag acctgcatca
    39961 tatcctgccc acagatgccg aagcctgcct ggccaatacc ccgggggcct gcacccgttt
    40021 ttacggttac atcgaaaaat acaaccaggc caggaaccaa atccatgaac acctggagga
    40081 agacctccgg gcggtgggac atttttggtc tgacccgtcg gtggccgccg gcttaaaaga
    40141 aatttacggg tacgtcctgg acaccattca tgaaccctat ctcttcgaaa aaatgaacaa
    40201 aacggtcatt caacccatca aacagaggtc ccaggggaat gccggcctca ataaaacgaa
    40261 aaaacaggtt tcggaagcag gccatgccct ggccgccgat ctggaacccc tgatccgaac
    40321 cctggatgaa aaaaaagcat ttgtttttaa cctgatgaca aactcgattc aatgattagc
    40381 cccaagcccc gcaaatcctg gctacctgcc acaatgctgt tgattttgat tcaatgcttc
    40441 ctccattccg gctgcaaccc ggactgcacg cccctactgg gcttgcaggt actgcctgcc
    40501 gtgggagaga gcgggaccga ggtcctgatc aaagccactc ccctggaatc cctggtcagc
    40561 caggatttgg aaatcaggtt taattcaaaa ccggtgaccc agaaaaaatt tcatccccag
    40621 ctgggcctgg tggtgaccgt acccgaaggg gtcgagggcc aggtggacct ggtcatcgcc
    40681 aatccggatt gttcggagag actgcctttc aatgcggtcc cggcctcctt ttttgaagcg
    40741 aataaaagtt ttatcccccc gtctatcccg cagatcttta tacccatcaa cccgcttcct
    40801 ccctttcctc catccctgga gaatgcctgg ctatgccctg aaaacacgga ttactgcatc
    40861 tggttcaaat tcttaaagga ccccaaaggc aaatgcacga cctccattga tcccacgcag
    40921 agcttcgagc aatccacctg caacggcaac aataccgccc tgctttacaa taagaatccg
    40981 attttcggag tggtggacaa ggataaaaac ctggtccatt tctggattga ccggaccaaa
    41041 catcctggcg gcaacctggg gaccgaagaa ttcatcggca gctttatcaa tatcaacgat
    41101 acgccttaca aacctttcga acaattggct tgcagcccgg cggtcggctg gaaaagggaa
    41161 cgcaaccata tgatcctggt gacctccacc acgaccaacc gccaactggt gatttaccag
    41221 caggccttca acgatcccaa ttttatgatc gactgcaaat aaaaccggaa gacaccatgt
    41281 ttcgcatgca taaaacagtt ggccggagta ttttcctcct ggtcttcggg gtcttcggga
    41341 tccttaccgg gaatgctcag gagcaagccg cagaagaacg gttttcattc ggcaatatct
    41401 ccccggtcat tcttcccaaa gacgggctcg aagtgacggc caccggcaat ctgacctcct
    41461 attgggtgac ctcccagttt ggcaaagtag ttcttgaccg gtaccgcatt tccaggtcgg
    41521 aaaactacct gaatctgaat tatggctttt ctgaaagcgg gcgatgggat ctgggggtac
    41581 aggtccggta ctcccgtttg cggctggatg aaaacgcgcg caattctccc tttaaggtct
    41641 ttggctctcc ggatgccacg ggtatcggtt atcagtccat ttccaccgta gggctgcggg
    41701 tgagggccct gccttttgaa aatattccgg aatttaccct gcagggggcg gttcatttcc
    41761 ccgtagccaa agatatggag accaggtcct tccttggagc ggaccggatc cagcccgaac
    41821 tcaccgccac ttattttaaa aacctgagcc ccgattttta tctctatctg cagggccggt
    41881 atttattgca aatagccaat gaggaccacg accagaccac tcatttcccc ggtctgagcg
    41941 ccttcctggt caaatccttc cttgatcaaa agctctatgt gttccccggg ctgagttggc
    42001 ttggggctta tgaacaaaac taccaggggg gccgtttggt cagcgaagct cagtttgtca
    42061 tggtcagcct gggcgcccag gtccagccgc tttcttacct gggagtattc ctgaatgccc
    42121 agaggcccct gtattataaa tccggattga gtttttattc cgacctcgtg aaaagcagtt
    42181 attccgattg gtcgctgggc ttgagattta ttttatagtg agcgatagat atgccagatt
    42241 atagaaatcc ggcatatctt ttcctatgat ccccaaaaac gataaaaaac tcatccatgc
    42301 ctgggccatg tatgactggg ccaattcggt ttatgccctg gtcatcacct ccgcaatctt
    42361 cccggcttat tacaattcgg tcacccgcaa cggagaccat tcccgcgtct ctctcttcgg
    42421 tattcagata gagaatacgg cggcctatgc catcaatctg ggcattgcct tcggcatcgt
    42481 tgccttgctt tcccccttgt tgtcctccat ctcggattat tcgggaaatc aaaaaaattt
    42541 tatgcggttt ttctgttacc tgggagcggc cggttgtata tccctctttt ttttcaccgg
    42601 cagcgcccag gtgtacctcg gactcggtgg gatgatgctg gcgaccatag gctattccgg
    42661 cagcatcgtt ttttacaatt cctatttgcc cgtcctggcc acggaggacc ggcaggacaa
    42721 ggtgagcgca aggggctacg cttacggtta cctgggctcc accaccctcc tcatcctggt
    42781 gctgttcttc attcttaatc aaaaccggct ggggatttcg gacgaaaccc tccttccccg
    42841 tctttccttc ctgatcaccg gatgctggtg gatgggtttt gcacagatct ccttcaacaa
    42901 actgccccgg ggcctgaatg tgaaacctgt tgaaaaaaaa tacctcttcc acggctacct
    42961 tgaaatgctc aaaatctggc gccgtctgaa aacggaaaag aacctgcgca cttttctggc
    43021 cggctttttt ttctacatca tgggggtaca aaccgtcatg ttcatggcag cttcattcgg
    43081 agaaaaggaa atccatctga acatgatgaa cctgatcctc accgtcctga tcctggaata
    43141 cattgccatt cctggcgcct ttctatttgc atggatctca aaaaaatggg gcaatatccg
    43201 ctccctcagc ttggccgtga tgacctggat cctcatctgc atcgccgcct atttcatatc
    43261 cactgccttt catttttacc tcgtcgcctt atttatcggc ctggtgatgg gtgggattca
    43321 gtcgctctcc cgttccacct acagcaagct gatccccaag acgggaaaca atgcgggtta
    43381 tttcagtttt tatgacgtct gcgaaaaaat ggccatgatg tgcggtttgg ttctttttgg
    43441 ttacctcgac catgcctccg ggagcatgcg cgggtcgatc ctggccctgg ccctttggtt
    43501 tataatcggc ctgacctttc ttatatccct gtttaaggat gcccctctgc cccataccaa
    43561 tcctgaccgg ccatccatta gttaatggat tgtcctttta attccctgat cagattgccc
    43621 ttcttcccgc gggataccgg caatttactt ccgttacgaa ggaccacctc tccgccatcc
    43681 ccctttagat agcggtagat atgttccaga ttgaccaggt acttatgatg gatgcggcaa
    43741 aatccaaggg gccgcagttg gttttcaagt tctttcaggg aatgagtgga ctggacaaat
    43801 tgattttcct ggtagatcac caggcaaaaa ttgccatccg cctgaatata taaaatatca
    43861 tcagcggagg ccagttcata accgcgatga accggaatgg gaattttatt catagtgaag
    43921 ggttgtcttt tggtgtttaa atcttcacct ccggagctat tttaaaatta caaaaaacaa
    43981 aaaggtaatt ttctcgtcac cagatattct tgataaccgg ctctcctgat gagataacaa
    44041 gcgcctcttc aattgcaaat gataattccc ttcattcgca ttaattcttg aatcaggata
    44101 tttctatata aggtcgccat tcaacctcag ggcgctaagc aggttcgcct ttttacccct
    44161 tgaaacagcc agccgctcgc cgttggtgag ttcgacttcg cctccatcgc ccttgtaata
    44221 ccggacgatg tgggacaggt tgaccaggaa ctcgtggtgg atcctgcaaa aaccacgtcc
    44281 ctcgagctgg cgttccagtt ctttcaggga ccggagggta aattttttag ttttttccgg
    44341 atgaaataga atggaataat ttccattgga tttgaaatag aggatttcct gtaataggac
    44401 aaactctatg ccttcgtgta tgggtatcgg aatacggttc atgcgcttta aatcaaaggc
    44461 ccggattgtg cggggctcct ggtccgttat tgcacggtat aaattttctg taaaaaccct
    44521 tgttttctgg ccctggacac cggaaggacg gtaccgtcgg tgagaaccac ctggccgccg
    44581 tccgccttct cgaatcggac cagctggttc agattgacta catattgatg atgaatgcgg
    44641 taaaatccgg caggcgaaag ccccgcttcc agttgtttaa gcaaacaagg aagaagggtt
    44701 ttgcaaccat ccgcaaaatg cgcccaggaa taattgcctt ccgattgcag ccacaggatc
    44761 ctttttcttt ccatccataa aatcccctgg ctacagggca gggccacccg ctctgtccgc
    44821 tctggtatcg tgtccataat gatttcaggt aatttgtaat tcatttatac cccgaaaccc
    44881 taaaatgtta cccggttcta aactttttta taattttaag ctgaccacac ccaccattga
    44941 ataataagat ccgggccgaa aatgaacata tcctggaggg cctccgaagg ggcgatctgc
    45001 gcgtcattga aaaggtcttc agcacctgca agcccgctgt aattcaaatg gtcgtccggc
    45061 agggagggaa cgaagccgat gccgaagatg tatttatgga cgcgtttgaa gccatctaca
    45121 accggctgca atccaacggc ctgacgctgg accgggcttc ttttaaaact tatttcatcc
    45181 aggtctgtct ttttcagtgg tccaagaaaa tccggaaaaa aaaccgcgtc catcaggtaa
    45241 caaatcctgc cggagaggta caaaccggga tggaagaggt gcatgaggcc ctggcagatc
    45301 tggaccgtaa gaaaatcgtc gccgaaaaat tccaattgct ctccgaggga tgccggcaat
    45361 tgctcgactg gttcatttcc gaagacaaaa atatttctga agtagccctt ctgctgggaa
    45421 tctccgaagg cagtacccgg aaacgcaaat atgaatgcaa ggaaaaactg ttccggctgg
    45481 taaaagagga ccctatgtac caagaattaa aatttccagg atgatcgaag aggaatggga
    45541 tgaagccgtt gaccgttttc tcaggggcca aatggacccg gaagaaagcg cccgttttgc
    45601 ggaccggttg aaaaacgatc cgtccctcca ggaatatctc cggttgaacc aggcgctgca
    45661 ccaggccctg aggaattctc cttccccttt caggcagacc ctggaaagaa ttaatgtctc
    45721 ttctgacttg cgggtggctt caaaacgtgg ctggatcctc gtggtggtga cggcaatcgc
    45781 cctctccatc tttgctttcc tgggcatata cctgaacagg aaaaagaatt ccggtccgga
    45841 ggccttgtac ctggcttata tgaaggcccc tgcttcgctc agcccggacc tggctcccga
    45901 ccggaccggc gaagcgcaat ccagaagcat ttcttttcaa gattccgtat tgatggaaac
    45961 ggaccgtctt tacatcaatg gacaaatcga ccaggctttg caattattgc agtcgaccca
    46021 ggaagatttg ttatccgacc ggatgctttt tcagaccggc ctgcttttgt taataaaaaa
    46081 ccaagccgga gaaacactcc gggtgtttga gaaaataaat gcctatagcc ccggtgaaat
    46141 cctatggtat ggagcgctgg ctcatctgaa gcaaggggag ccatcggcag ccaggtcaaa
    46201 gctggagctc attcctgctg agagccgatg gtactcgcaa agtcgggaac tgttgtcccg
    46261 gctttagtgt tcaggtctgt ctgcgaagcc aaaaggctaa taagacggtg cctgccaaaa
    46321 gcgccgcaag gatccagtaa ctaattgaat ccatgccctc taagatcaaa ggttcattgt
    46381 cccctaccag gaccagcccg gcccaataat agggatgttt taattccggt atggcgttgg
    46441 ccaggtaatc ctcctttgct ttacggaggg cgtctgcctt gccgaggccg gtttttaggt
    46501 gtttaaaaaa acttacgatg aggtccttgg tactggcatc gttggcttgc cagagcgaca
    46561 tgactacatt tggacaaccg gcggccttaa aagcccgtgc gagcgacatc accccttctc
    46621 ccttcaagta tttgcctttt ccgctgttgc aggcgctcag cacggagagt tccgcgttta
    46681 aatgcatcct ggcgatttca taagcataaa tcgcttcatt tcttccggga ccttccggat
    46741 tttttaatat cagctgggag tattcagggt gtttgtcatc cgcgatggcg tgcatggcca
    46801 ggtgcaggat cctcgcatta gccccgttcc ggagcaattt tgatttatct acttccaggc
    46861 cggtataggc ttcccccttc catatattgg ctgattccaa tacttctggt tcgttaaaca
    46921 acagattacc atatgcctcc cggttggcgg catacaaagg ataggtaatc agggagtcca
    46981 atcctcgctg aaggaaaatc tcatggccct catagcgggg agcaaacccg gcatagtttt
    47041 catccggctt cagcaaaggg ggttccttca acagcaaacc gctggcgtat tcgtaccgga
    47101 tggtgcgttt gcccagcggg gaatcctcaa atccccttat gctggattga agacattcaa
    47161 aagggaggcg ggccagaaag ccgtccggga tgacgatcag gtggttcccg gagatctggt
    47221 cctccaccgg tttgattaat aaatcgtata atccggcgga ccatacacct aaggaatccc
    47281 gggccgtttg atcagtttcc ggattcaaag catctgagaa tttaatagcc agagcttcca
    47341 gtccagaatt ggcttcctgt tttatgatcc tgaattgact ttgattcatg tgcaggaggt
    47401 ataaatgttt tgtgccggta aaataatcca gcaggtcagc ctgctcttgt tttaaataat
    47461 cgcgaagccg gtcgaaggga aaccggtcca gggaataagg gtttgggaca tggaccgtct
    47521 ccattggttc cttctcggca agcatgacca actggcctgc cagttcagcc tgtgtttttt
    47581 tcagattaaa caattttaaa gtatccttca ccggcgaagt ccgctccatt tcaaggatct
    47641 gatcccggag gtaggcaatt tccgttttaa gttgttcggc ctggctcctg ggcgccgcct
    47701 cctgcccata ggcggatatg ttctgcttgt ccagcagttc tttgatctga aatgccttga
    47761 ccagatccga aatatagaat gcttccctgg cataactttc gtttcctgtt tgttcataaa
    47821 gatgggcgca caattcgagg gcgtcctcat aaaacgggta cagataatgc aagcggttgg
    47881 ttttggactg gcggcggtag agttcgtcct ggaggcgctt catcgaagca tgaaaggttt
    47941 gcagtaaacg atacccctgc tccagaacgt ttattgaatt ttcctgtatt ccaatggaaa
    48001 ccatactctg gattaatggc ttcagaaaat cctgaacccg gagaacctcc tgtccggtaa
    48061 acctttccca ttgaaccagc ccggcaacca aagaatccat tgaaggaact tgatgtattt
    48121 ggtccaggat ttctttacaa aatcgtatac aatctccggg ctgtcctccc ttgagcagtg
    48181 ccctggcttt taattccaaa agaacaaatt cagggttgct tgaagaaact acgggatctc
    48241 tgagggatgc atcgaccaaa tttatacaat cctggtattt acctgttttt aaaaaaaagt
    48301 cagccaatcg gtatctcaga aaatgcctgt ttaccaatgc ggcgccggat tccgatttcc
    48361 ggaattgcgt cattaattct tccgctttct tccactcccc atgatacaaa tgaatggctg
    48421 ctctactatc caaagcactg cgctgaagga agtcatttcc cggttcgtgt ttcttaatca
    48481 gggaatccgc caggtcagca aggtaggtgg ctatttccgg ctcatcattg ttcaggtgta
    48541 tccagacttt attaatcgta gcaccaatat attgcctggg gatattgctt aaaagcaggt
    48601 tgcgaaccac gggcggacgg ctgttcgtgc ccataaattg tttatagaat ttggcgtccg
    48661 gaatatccga atgtctgatg acaaagttgt cgtacagggt cttcaccagg gattgcatcc
    48721 tgcttgcaaa aatctccgca tgatctacgt cgcccatttc ccaataaaaa gaggccatat
    48781 tattatagac attcaccagg aaattattta aactgtccat ggtgcgcacc ctgatttcat
    48841 aggcttttaa aaaatactct ttggacttta ccagttcgcc gtaattcctg taacagtttc
    48901 ccagcttgat caggcatttg gtggcaatga ggtggtcctc ccccagcttc tttctgcacc
    48961 agtcaaggga caggttcagg tatttaaagg cgctgtcgtt ttcctgtttc agctgataga
    49021 ggtatccgac attgaaataa ctgtcaatat aatagatcca ggtcagggta tctgctgtat
    49081 attgaagcaa atggtcgaaa cgctgcacac agtcctgcgc gagcatcagg gcgctgtcca
    49141 ctttatacat ataggcataa atctccgccc ggtgaaactg gtcaaaagct gaggtggaat
    49201 cctgaagata agaggcgggc ttatcaagca acaggggctt catcccctca gccggcgttt
    49261 ccggattttg gggctgggag caaaatgtaa ataaaaacag gctcgccgcg aataaatatt
    49321 cacgtaaagg gaatttcata gcaggatgtt aagtcactta agatagggaa ttaaatatta
    49381 attaagaatt aagaattaag aattcaatta caataaaaaa aacccccgtt acttgcataa
    49441 cgagggttta aagtagtctt aaaaaacttt acgatcctat ttggtatccc accatacctt
    49501 ggtggtatat gtgtctggcg tcacccctcc tgttgccagg ttggggttgc tcgctgtttc
    49561 actggttgga taagggatcc gtacgggaat ggtgccattt gtgatgttgg agggatgctt
    49621 ggcggctacc agcaccggga aatccagttg cctccagtaa gaccacacct cataccagtt
    49681 gaggaaattg gccacccaaa gctggtcggc aatcatctga atggcaggct tcgtcactcc
    49741 atattggtaa atgctgagat aattggtcac agcggcatcc gatacggtca gcgaggcatc
    49801 gtgtatggta tactcttgca tggctgcttt cacgcctttt tcataatgct ccttgtccgt
    49861 gccggcaacc ccaccgaggt ttttcagctt tgccagggca agcataaatt caacctctgc
    49921 ataacgcatg attacatatg gttcgtcgtc ctgcatcagc ttgacattga ttttgctgta
    49981 ggtcgtgttg aaatccacgt ttctgccttc cagggcatcc acgtccgttt tgtccaaacc
    50041 atgaggcatg cccttttggt ttaacggatt ggcgccgtcg ccgatgatcg tgaaatcatt
    50101 ggcggtccag ttgccgatcc ctccgctgat gatcatgaga cggggatcgt catcggccac
    50161 ggaattggga tccgtgcctt tcaggaaatt aatcagggtt tggcccaggt agggcgactg
    50221 accgccgtct ccgggataga aggcccggga tatcccgttc tggttggtcc attcgctggg
    50281 gctcagagcc atggggacaa caaaattatc cgcattgctc gtcatcacgc ctccggtcac
    50341 cgccttcccg gcataggtcc ctgcaagggc cccgtcggca tcggctgcac gcagggccag
    50401 tcggagcatg agcgaataag caaacttttt ccattgggtc acatctcctt tatatacaaa
    50461 atccgctttt gcaaaactgg cctggtcttt atcggaagcg ttgagtgctg cggcggcttc
    50521 atccagttcc ttcagaatgt ccgtataaat ggctttgccg tcatcgtatt tcggaaagaa
    50581 tatgcctgag gtcccaagat tggcctcgga ataaggcacg ttgccataca tgtcgaccag
    50641 acgtgcatag gtccaggctc tgaggatacg ggccgcattg cgggtatttt gtttgttgcc
    50701 ggcatcaaaa ccgccgtcac cggtttgttt gagcacttcc gcgatgtttt tcaactggtc
    50761 gcggtaagac atctcaaagg gcgcggcgcg ggtctcccca ttgtcggtgt atttatcacc
    50821 gggagaaatt ccaccgctgt tggcgttgcc caggtgctgg atggcatggg cgcacaggcc
    50881 gatattggtc ctccagtcga tgtaccggtt gtccccggaa gacccgttgg aagcgatacc
    50941 caatagggcg gctgagaata ggaaattcat atcaatctgg ttgaccgcct ggggattgat
    51001 gttcagatcc tgcagttcgt cggtattgca acgcaccagc aggacggata ctatagtcag
    51061 aaataataat tttttcattt cgaattgatt taactgttaa aacccaactt ttaaattaaa
    51121 accatagctc cgtgtggaag ggaatccgaa ataatccatg ccctgggaat tgctgaccgt
    51181 ataagaagat tccggatcga tattgtccac attcttgtgc aggatcgcca ggttccttcc
    51241 tacaaaggaa aggctgaggt tctggaacgg agttttgctc aacattttat tcgggaaatt
    51301 atatcccagg gtaagctggc gaagcttgat aaacgaagcg tcgtaaacat acatgtcggc
    51361 tttaccgttt tgctcactgc cggtggttga ccagtaacgc tgggcttcct gtggagtgag
    51421 atcacggtcg atgggctcaa atacgttcgg agaacgttcg atcacccctt tgacatggag
    51481 gggtgcttca ccctggcgtc cgatcagaga ttgtttatgg taaccccatt gggtcagcct
    51541 catattggtt cccgagaaca gatcaccgcc aaatttgaag tcaaccaatg ctccaaggtt
    51601 aaaattattc caggtcaggc tgttgtttaa tcctcccgtc agatcagcca ctccatttcc
    51661 gatgatctgg taattgggat ctccgatcgg gcttccccac tggtcaaata cgagattgcc
    51721 gctggggtct gttttctgaa cccgcccggt gatcatgccg tatggatagc ctacaatatg
    51781 tttgatgaaa acgttcctcg tacgaggctc ttccaatacc agttcttttg acccttcgat
    51841 caggcttaca acttcattgt tgtttttagc catattgaga gaaacgtccc aggtcacttt
    51901 gccttgtatg ggggtgaccc cgatgagggc ttcaaatcca cggtttgtca atttgcccag
    51961 gttcacagcc gtggcatcaa atccggcagc tctggaaatg gtagcaggga ggatgtcgtc
    52021 ggtcgttttc tgggaataat aagtcaggtc tgcataaacc ctgttctgga acagacggat
    52081 atctacgcca aattcaattt cggtggaagt cagaggctta agattcggat tgggtatggt
    52141 ccctccctgg ccgatggcaa aggcaaaccc accaagaggg gtaccaacgt gcggtgcgcc
    52201 gttcaggccg taggtaatgg ctgtagaata agctcccacc gtagcatttc ccacctgggc
    52261 ccatccaccc ctcagcttga gataactgat gaaagaaggc aaatttttga aggcgtcgga
    52321 agccacaaaa ctggcctgga cggaaggata aagaatactg ttgaattccg ggttcaatac
    52381 ggaaaaccag tcgttcctca cagtcccggt caggtagagg taacctttgt agcccaattc
    52441 agcctggcca aggagggaat tgatccctgt ctcgctttgg ccaaatcccc agttcctccg
    52501 gacggaattg ttgataaaag gttggaaggg aacgttaaag cccccgtcgc tgctgatatt
    52561 gatcacatcg gattcgcggc gcatgcggtt gccaccgccg aatacattga ccgaaatatc
    52621 cccgaaggtt ttattaaacc ctacggtata ttggaaattg gtttcctgta cgcggtcgat
    52681 gccttcactg atggccccgg cgcgattata tccggttccc tgcggggtca ggccttttgc
    52741 ccttctggca tatttgtcca aaccaatctg gccttccaca taaaggaagt cagtgatgtc
    52801 atacctcagt ctccctgagg aaatgacgcg gtcccttttg tcgttgttga tgaattggta
    52861 ggcagcccag taacctccgg aactccactg gtcgttgacc gggttgacct gcaattcttc
    52921 accagggata cgtccccagt aggagagcac gttggcagga atgccttcag cgggtatggc
    52981 gccgatttta ttcgggtccc cgcggagcac ggagtaggga atgttgggag ccactttcca
    53041 gacgttttga atcccgttgc ccggagagtc tgaaagggtt ggccggtttt tattgttttc
    53101 caaagaataa aggattttgg catccatggt gattcttttc ccgaatttgg aattggccgc
    53161 cagcgataca ttggtccggt caaatccggt attgggaagg atcgttgagc ctctgaggtc
    53221 cgttgcggat atgcggaaag aggaattgtc cgtaccgccc gtaaaggcaa ggctgttggt
    53281 aaaggaggta ccggtttcaa aatattcctt aaaccggtcg ccggcatagg aatagggcct
    53341 ggatatacca tcaaactgga tcacggatga accgtcaaat ttgggtcccc atccctggtg
    53401 accccagttc ccggcttcct gtatggtgga aggtttggtc gataccccat tgggcaaagc
    53461 cccggtaaaa ccgcctgatc cgtaatcatt ctggaggtcg gacaaatcat tgagggtttc
    53521 aaatacaaag ttggaattga attccactcc gattcctttt cttttggctc cccttttggt
    53581 gatgatgttg atgaccccgt tggctgcacg agcgccgtaa agagcggccg cgttggctcc
    53641 ctttaacacg gtgatggatt cgatgtcatc ggggttgatg ctggacatcc cgtccccttc
    53701 atcggaacca ccccaaagac cggcctgtcc aaagcccgaa ttgtccatgg ggacgccgtc
    53761 aactacgtat agcggctgat tgcttccgtt gagggatttg ttcccccgta taatgaccct
    53821 ggaagatccg cccgggccgg aagccacctt gctaacgtta acccctgcca cgcggccggt
    53881 gagggcgttc gcaatattgt tttccctggc ctggataaaa ttgtctccgc cgacttccga
    53941 cacggagtaa cccagcgcct ttttctcccg gctaacaccc aaagcggtaa ccaccacctc
    54001 gctcagggca acgcccggct gcatggttac attgatggag gactggttgc ccaccacaat
    54061 cacctgctcc tggaatccgg tgtatgtaaa tctcagcgat gcattgtcgg gcacgttgtc
    54121 gagggaataa ttcccgtcga aatcggtcac tgtaccctgg gtagtcccca gaatgaggat
    54181 agtaacgcca acgagaggtt cgctgtcttc tccattggtt actttgcctg tgatcgtgcg
    54241 ctgggcaatg gcttgccacc cgcagaatac aagcaggatc agtaaagttt ttttaatcat
    54301 aacaatacaa atattagttt tttaaaatct gaataaagcg gagtgttttt tacctgaaaa
    54361 aacgtataag agacctctaa tgtacctcat tgttaagaaa ttatttagtt ttccaacaaa
    54421 aggattaaaa aattcaaata ttggataata ccgtgtaagc agcctctgaa aaacgaatag
    54481 tgagtgtaaa attttaacta tttctgccta aggggtattt tacaacgatt gggcaggtgg
    54541 gcttgtgccc ttccggatta aaattatttg cccttttttt ccgccgggtt ttgccgaaat
    54601 acccggagaa tgtcatcgtt acagccagcc agaatgaccc tgcccgaagg actgtccatg
    54661 gaagccaggg atttgacgtc ccaaggcata aatattcctg atttgatgcg atcaagggcg
    54721 gtgaatccgg acttgccatc cccaagcagg atcatcccat agccggcatc gttgcgggtg
    54781 gtctctatct cggcctggta aaggttgcct gccaggagga ggtccatatg accatcctta
    54841 ttgaaatctt cggtgataat atcgtttaca gaagacaatt gggccattgt gggtaaaata
    54901 tggggtctga ataaaccatt ccccagattt tcaaaataag tgcttgcaaa agtctttgct
    54961 tcgagatgca gtgcattttt caatttatcc ttgccataaa cctgagccac gttggatgag
    55021 gcaaacatat cgtaggttgg aaatttttga ctgagcgcgg ggacctgttc cgaagaacat
    55081 tgcctgcccc ggagcggaaa ctgcaccccg aaattgtaat aggtcaggac cacgtctttc
    55141 gaaccgttgt cgtcgaaatc ataatagtac acctcgaagg gttcttcttc actggctttg
    55201 tatttataat tcagacccag gttacctgcg atcaggtcct gatcgccatc ccggtccata
    55261 tcggcggctt taatgctgaa ccaccatccg gtactggatt gaagcgtgaa atcatccgta
    55321 ttcctgacca actggtcccc tttgttcaaa aacaccagga taggggtcca ttccccggcg
    55381 aggacaaggt cgggtttctg gttgccgtcc agatcggtcc agaccgcatc gttgatcatg
    55441 cccaggtcct taaatccagg ggccatggcg gcggtgacat ccgtaaactt cccctggtca
    55501 ttctttaaca atgcactgct ggcagggacc gggtaggccc aggtcgtatg ccttcctgct
    55561 atgaagaggt ccgggtcccc gtccccgtca aaatcgcagg gcctcacctt ggaaccgctg
    55621 aataggagtt caggtaaaat gccttcaccc tttgtaaacc ggccttttcc gtcattgacg
    55681 tagatccggt cctggtagga cttggaaccc ggcctgaatt cattgcctcc gctgaccaca
    55741 taaaggtcca agtctccgtc caggtcaaaa tccaggaagg ccgagcccat atcttcatga
    55801 tttttatccc tggtaaaaca atcattgatc agttcctcaa aactgccgtc cgccttctgg
    55861 caataaagca cccccggtgt gcctgccgct ccgccgacaa acaggtccgc caggccatcc
    55921 gcattgacgt ccccggtgga caataccggc ccgaaggagg acattttatg gggaagcagg
    55981 acctggactt tataatcatc gaagctgttt tcaatgtgcc ggtgggaaaa gttgatctct
    56041 ttattgattt ccgtaaaaag cggcgccggg accgttttgg tttccggttc ggggaaggct
    56101 gcggtcgaat aatttatttt gagcacctgg tcggctttga cgtcggtaaa caaattttct
    56161 ttcccatccg gccagcgcac ttttactttt tttatgatcg ggtttttgcc cgttccgaaa
    56221 tgggcaatgg tttcggaagt ggaatacatg ccccgcactc ccgtaatctc aaaaaactgg
    56281 ggaccggcgg tggtttcaat ccagatcttg gtacccatgg ccgtgatatt cccggcatcg
    56341 gctaccggct ggatgcgcag gtagtggttg ttttcggtgg cattgttgcg gtaaatggaa
    56401 gcgaggtcgt tgatattgct gacgaccagg tccaggtctc cgtcattatc gaggtccgcg
    56461 taggcggctc cgttggaaaa ggttttttcc gtcaggcccc attcttccat ttgtttggaa
    56521 aaacggaggt cgccttcatt tttaaaaata tagttcctca gtttgacgga aggggtcagg
    56581 gaaagcgctt tgttgatatc cacgatatcc cagatggata ctttctgatc cagggtggga
    56641 tgctccgcca tatattcggc catggcagcc tggatgttgt ctacaaaggc tttgtgcgcg
    56701 tccttattcc ggatgtcccg cataaggccg ttggttataa acaaatcctt ccagccgtca
    56761 ttgtcaaaat ccgcaaaaag cgaagaccag ctccagtccg tggtagccgt cccggctagc
    56821 tgcgcgatat cgctgaaatg ggcccctccc gaattcaact ggagggtatt gaacatgtac
    56881 tggtaatgcc cgctgtcctg gaccactttc cagaacgcgg cgggattcat accgctcata
    56941 ttggccttca gccggtagtt gtcctccgca accatatcca cgacgacgag gtcgagcctg
    57001 ccgtcgttgt tgatatcccc ggcatccact cccatgctga aataggaaat gtgcttcagg
    57061 ttgtcgtgaa ttttattggt gaaggtgcca tcgccattat tgataaaaag ccagtcgggg
    57121 atccagaaat cattggaaat atacaggtcg gtccagccgt ccccgttcag gtcgctcgca
    57181 gatacactgt tggggaaacc cgtttttttc aggccggcct tttccgtgat gtccacgaaa
    57241 cgatccccca ggttttcaag cagtttgatg ctgtattcct ctttgatgag ttcggtgccg
    57301 taataactgg aatagtcacc cggattgggc ggttggttga gcaggaggag gtccgggtcg
    57361 ccgtccttgt cgtaatcgat aaaactggcg tgccgcgtcc gggcggaatc cgccacccca
    57421 tattgttccg acttttcagt aaaggtgctg ttcccgttgt tgatgtacaa tttgttttta
    57481 cgcagttccg gtttgtcgtc gtacaattcc cgggtgatgt aaatatcaat atacccatcc
    57541 ttattgatgt cttccatgag aactcccgaa gaccatccac cattgctgag gatgcccgct
    57601 tttttggtaa tgtcttcaaa ctggaggttg cccttgttga gataaagttt atcctccacc
    57661 aggttgccgg ccataaatat atcctggagg ccatcgttgt tgatgtccgc gatccccacc
    57721 ccggcgccgc cataaaaatt ggaatagatg gtgatgttgg cttccttggt gtccttgatc
    57781 tggttgttga accggatccg ggtgtggtcg ggtgaaagca gggtgaacag ttttttctcg
    57841 ggaggcgcgg aagagggggc cacctcaaac gaaatacttc cggagaatac cagtaaaaga
    57901 aaaagaaagt acttcataat acggggttac ggaaatttaa agttttcaaa aataggatat
    57961 tcggcctaca ttgcctggag aattttgttc aattcaatcg atgccttaat tatgaaacga
    58021 atgatggtcc ccagatgcgt gttgatgctc tttttttgtc ctccattcct gttcaaggtc
    58081 tttgctaccg gacccgctgt gctttttttc agggccaacg cggacacggt ggctaaattc
    58141 gaaaaatttg agatcagcct cgattttaaa gccgatttta ccaatccctt tgatccggcc
    58201 caggtggacc tccgggcttc gttcacttcc ccgacaggaa aggtctggca gattcccggc
    58261 ttctataatt acagcagctg gacggccgtc tggatgatcc gtttctcccc cgatgaaacc
    58321 gggacctgga aatacacggt gagcatcaaa gacaaaacgg gcgtaaacga cagcatcaac
    58381 cgggtgttcc aggtgcgccc ttccgctcac aagggcccgg taaaaatagc tgcaaacaaa
    58441 cgttttctcc agcatgccaa cggcgactcc tatttcgggg tcggcctttg gtataacgac
    58501 agttacgcgg cctataacca gggcaggatc aacccgcagg aactggatga gctcaaacga
    58561 cttggggtaa attttatcag caccttcatc accccgcttg aaacccatgg aagcgggctg
    58621 ggacggtacg atcaaagcat ctgcggcagg ctggatgaag tgctgcagct ttgtgaggaa
    58681 agggacatcc agctcagcct caatatctgg tttcattcct acctctccga aaccgtatgg
    58741 ggagggggca atatccggtg gaataccaac ccctataaac aaatcacctc agccaaagat
    58801 ttttatcgca gtgaagaagc ctggaaattc cagcaactgc tgtaccgata tatgatcgcc
    58861 cgctgggcat acagccggtc cctcttgctt tggtttgtca tcgatgaagt caacggcacc
    58921 gacggctggg tttccggcga cagcctgatg gctgcccaat ggggtaaaaa agtacatgat
    58981 tattttaagg cccacgatcc ttatggccac ctcaccacgg gtacccgcag cggaggcatt
    59041 ggtgaattct ggcatgaagg ctaccagatc tttgatttgt ccaaccggga aatatatgaa
    59101 gcccagggat ttcccattgt ccggagcggc aggctggaca gcgccgatac ccatcccctg
    59161 gccctgagtt atatgaatta tgccggccaa atagaaaggc tctggaaagg ttatgaaaaa
    59221 ccggccatca taggggaaac cggctgggac cacactttct atgaacccag catgccgggt
    59281 taccaggcca tgtatcacaa tgccctgtgg gtaaccctcg caaaaggcac cgccatgacg
    59341 cctttttggt gggcccactc cggctacctc aatgacaaca tcatcaccaa ccaggtccgg
    59401 aatatcgccc ggttttccca ggaaattccc tttgcaaaac tcagcaagcc aaaaccggtg
    59461 gaagccatca cgggaggcag taatgcctac gccatgagca gtgaccagct gatctatggc
    59521 tgggtggtca atccggattc cgatgtcagc ggggcacagg taagtttgac gttgacaggc
    59581 agtcccgcct caaaatataa ggtcaggatt taccatacct ggcggggggc ttttatccag
    59641 gaatccgagg tggtcccgca gggcaatcag atccggtttg aaataccggt gctgaagatc
    59701 cagggcggac ggggatttta cattggacag gacgcggctt ttatcctaag ccccatagaa
    59761 taagccgtgg cgaactcgaa gtcttcccaa ctgctaactt gccaactagc caactagcca
    59821 acttactaac taattaacct tccacataca tcctctcaat cgcatccttg tatttctcca
    59881 gcaataacct tcgcttgagt tttaaggttg gtgtcagttc tccctcccgg ccatcggatt
    59941 tgatgggaac ccatacctcg ggcaccaatg tgaatttttt tatttgttcg atatgtccaa
    60001 aaagcggatt gtattggtcc acgatctgtt gatagagttt aataacctcg ggaagcttca
    60061 ccgcctcttt aaggccggtc cagggtattt tttcttcttc aaaccagttt ttaaggcttt
    60121 cttccgcagg gacgatgagg gcggtcacaa atttcatccg gtcgccgacc agcatgatat
    60181 tatctaacag gaaatcttcc tttaatttgt tttcaatggg gctgggcgct acatatttcc
    60241 cacctgaagt tttgaacaat tcttttttgc ggtccgtaat cttcagaaaa cggatgccgt
    60301 ccttgccggt gacaaaagtg ccgacatctc cggtgcacag ccaggttttt ccatcaatga
    60361 ccttaagcga ttcggcggtc cactccgggt gcttgtaata accgatcatg atattgggac
    60421 cggaagcgag aatttccccc tccccttcct tatatatggc ttcgctgttg tcgagccgca
    60481 cctccacgtt tgacatcagg gggccgaccg tcccgatcag ggctttatgg ggttcaaaca
    60541 ggctgatgct gatgccgggc gaagcttccg taagtccata ggcttcccgg accgggatgc
    60601 ccgctgccga aaaaacgcgg atgatccggg tggggcaggg cgccgaaccg gtgaccaccc
    60661 ccttgacttg tcctcccagg gcagccctcc attttgaaaa aatcagttta tcggcgatgg
    60721 cccactggat ggctttcagt cctgtgggtt tgaaatcgaa ttcatattct tcggtgaggc
    60781 tgatggccca aaaaaataat ttctttttaa tcccggtgag gtctgaaccc ttattgtata
    60841 ttttttcata aaccttttcc agcaaacgcg gaacacaggt aaaaaaatga ggactgaccg
    60901 atttgagatc gccttggtct ccacccaggt tgtccgtccc ggtctggtat acattgagcc
    60961 cgacataaac aaaggcatag gtcgcagccc tttcgaagat atgacagatg ggcaggaagc
    61021 tgagtacttt ttccccgcag cttaccggaa ggatgtcttt gacttcccgg atgcaactgg
    61081 ccaggttgaa atgggacaac atcactcctt tgggcttccc ggtggtgccg ctggtataaa
    61141 tgatggtcac caggtctgaa ggttttatgc gggcgatgct ttggtccact tcaccggtgc
    61201 ggtcgctgcg caataaatcc ttccagtgga tttcgccgac tccttcatcg atgcctatga
    61261 tttgttttag cgcaggaagg tttgcccgga catttccgat tttttggtcg aggtcgcctt
    61321 ttccgataaa aacggccttg caggcggatt cggtcaggat atattcatat tcacccgggc
    61381 tgatggtcgg ataaagcggc acggagacca tgcccgcgta ttgaatggcg aggtcaatga
    61441 cgatccattc gggctggttt ttataggaga tgatggccat attgtccccc ggttcgtaac
    61501 cggagtccag aagaccctgg gccaattggc gggccagttc aatgatctga gcggtgctgt
    61561 aaaatttcca ttccccattc tggcgcgatc cgaacgattt ttcctgcggg tagtgtttta
    61621 actggtaatc aaaaaattca aaaacgcgct caatcgtcat gggtcagaat ttaattggat
    61681 tcatgcagta tccggatctg gttcaatcgt agcaatatag cgaaatttag aaaaaagtaa
    61741 gtccctactt tgtcggtttc caccatatca ttgaaaaaca ggatgacgag gatggaggac
    61801 atggacatgg tggccgcaaa gccccaatgt cgcagcgccg gaagctttgc ccggagcagg
    61861 ggctgggctt ccagaaaaaa caaccagatc agtaccagga aaaagatcag tccggggagg
    61921 ccttgctcca ccgcggtcat caggtaataa ttatgaatcc cgctccggtc cgggttttca
    61981 ctcacatagg ttgaaaaacg gtgaagcgta taggcgggat aagcggaata aaaacctgcc
    62041 gggccaaatc ccaccaggta tttttctccc accattctca ccccggccac ccagcggtaa
    62101 agcctttcca tggtggagat atcctttccc tgcggagtgg cttcaagcag ggtgccgaat
    62161 tccacgtggg tcacggcttt gttgtaatcc ggagccagtt ccaggtaacg gttacccgaa
    62221 gtaaaataaa tcatggccgc aatgcccagg gccggcagga tcaacatagc cctgttgacc
    62281 catttccatc ttactacagg ataaaccagg atcaccgccg gcagggccag gtaggccgcc
    62341 cgggtataag aaaaaaaaat accggcaagc aggaccagga ccagtccgat ccataccatt
    62401 ctgaaaaaag ccttacgctt taacagatgc cagaccggcc aggcgtaagg aagaagacag
    62461 acgagcaggc aggcgtaatt cacatggttc cggtaaaagg gccgcaccgc ggtattgatc
    62521 tggtcaaagg caaatcccga accggcatgt ttccactcga tgatcatcac cgtgagggca
    62581 gccgtgccga tggaaagaag agcgaaacgg aaaagccttt tttcattttt cagaaaaaga
    62641 taggccagga tatagaatac aaaaagatac caggttttgg atatccagta tttcaacgaa
    62701 acccatacgt cctggctgga aaggctggta aataatatcc acaaccaatg tacggccagg
    62761 gcaaccgtga tggggtgttt cagaattttc cggatatccg cggtcgcagg atggagcaca
    62821 tacaccaggc cgaagccaaa caaccaccac atcaaaagtt catccggaaa atccgtggca
    62881 aaaccgcccg gcaggtccac ttcggtactc aaggggatac tgatcaataa caggtaaaat
    62941 atccagtgaa gacggtgaac gctgatctgg aaaaaaagca aaaacagggg ggccagaaaa
    63001 caccaggttt cctgaaaaac gattcccgcc aaggcggaaa tcaatacaaa taccggaaat
    63061 ccgtttcggt caatccacag ccggaatccc tcagcctgca taaaaactat ttccagaagg
    63121 atttcagaat ataatacagg ctgagcgaaa gggcggaaaa caaggcagcg gcgattacca
    63181 ggagactgcg tgtcggcctc gatttgtaga tgggtatttc ggcctgggat acggtgatca
    63241 gggcgggggc tgcagcgcca gccgtattga ccgcatgctg atagcggatc cggtcttcat
    63301 ttaactgggc attcaacctt tcaatattga ggtcaagcga ctggatccgt gaaacccctt
    63361 cattaaaatt ttcaagactg aaggaagcca cccctgcgtt tgacctggtg atgttccgcc
    63421 attgcgtttc caggccttta atgcgggcgg ccaggtacat gagggtatcc ctgggggcat
    63481 tgcttttttt caatacttca tagcgggctt gttctccggc caggtcgctg gacacttgtg
    63541 cggcaatggc ggacatggat tccgtctggg cctcaatatt gtaaatgggg taaattttcc
    63601 tcaaccggta caaactgtcg ttgagcgcat gcagcaaagc ctgcttttcc tggatgctcg
    63661 cccggtaggt gaccgatacg gcactcaggc gatcggtgat cagtttgacc gcagcccggt
    63721 ttaccgattc ccgcgcttca ttggacatgc tggcagccag ggctttgtcc tggtcttcta
    63781 tgctcagttc gatggcatcg tatttggttt tcacgacttc catccttttg tccagttttt
    63841 tccggatttt atgatgggca aaaggacggg tggaatccat cttataatgc cggtagaggt
    63901 caaaagtgga aatcaggtga tctttgattt cgttggaatg acaaagagtg aggagccggt
    63961 ccaggtcttc cccgctgcca taaaactcag ggtcggataa ggaattgccg aagatgagct
    64021 cgggcttggc caggtcgggg ctggccgcat aaaataccgt agtggcctta taataatttt
    64081 ttaaagtcag ggaaagcagc gccgcacaga tcccgaccag gagtgcccag gtgaaaatcg
    64141 gttttttttt atcccagagg attcggatag cgtccctgat ttgctccggt tcttccatac
    64201 aacgaaagta tgaatttctg gtcagccgac gcccgggctc aatcgacgcc tggtttaaag
    64261 actaaacgga aaacttgggc caggggcaac aaatttgagg agaacaccaa aatcaacagg
    64321 gccagcacat gaaccggaat cgcagccggt gcaggaagcc ctgaataaag cactcctgtc
    64381 ccggtcaaca cagccatccc cagaaccaat accataaggc cgaagtgctt ccattgaaac
    64441 cggatgatct tgcgtaaatg gatcaaaccg gcgagtaaaa aggcagtgat ggtttgggtg
    64501 accagggcca cccctgccgc cccggcggct ccatagctgc gcgtgaccag caaatgaccg
    64561 gctgcgttga tgcagatgga aattaaaaac aaccggttca tggcaccggc ttccttcacc
    64621 gcggttaaca gggtgctgag caggtagttg atggttaggg gaaccaggtt cagaatcgta
    64681 aggtggagaa tgtcttccgc cggatcggta taaagggcct gcatgatata ccggtgctgg
    64741 aaagccaggg gcattgcgat cagagaggca agcacaatca tcagtttcca ggcgaggtca
    64801 aaaagggact ggcgctgttc ccacaatttc tggccggtcg catgggcaaa catgggcatc
    64861 agtattccgg caaataaaaa accgagcata ttggccgcat caaggaggcg gtaggcggcc
    64921 gcataaatcc cggattgttg ggctccgtcg ttccgcagcc catttagcca gaccggctcc
    64981 atcctggaaa agaggaccat aaggataaaa atcatggcat aaggaaagca ttgccttaaa
    65041 atccctattg ccgccggcaa ggtgcccatc cgtaatcccg gctttgtttt aaaccaaaac
    65101 caaaacaatc cggtaaccag gggaatcacc agggaataaa actggatgga caggaaaata
    65161 aaaacaggct gaccttcaaa ggcagggaaa ataaacaagg caagaaggat aaaaattaat
    65221 ttatccgcaa ctgaaaagag gctgtccaga cggtaatgac ccaggccgga cacaaaggcc
    65281 ctgaggtaca agattcctgc cagtaagatc tggttgatcg cgatacccag gatgaggaaa
    65341 ggttgtgacc gcatggagtt cccggccaaa aggaaatata aaaccaggaa agccagagca
    65401 aagaaaggcc tcagccaagc cgtatttttc cagatttcca gggctgcgga ccggtcagag
    65461 gagatttttt gattgacata aaccgtcatg cccaaatcca gcaggatctg gggtatcatg
    65521 gacagggaaa acaaagtgaa atacaggccc caggcttcat tgccggcctg gttttgaaaa
    65581 tccctttcta taaaaagaac atagaacggt ttgatcaaca ggttggcaag caggagaaaa
    65641 ccgatattga ggacaaattc acgccggtaa atggccatgc gaacgggagt gtccgcaaag
    65701 gtatcattta ggtttaagaa aacattagca cttgatggca ttgcgtttgg atacccaggg
    65761 gaccagcgaa ttcgttttat tggtctattc gtacctttga cccttttatg ttcaaaagcc
    65821 ggcttctgct tccatgcatc ctgatttctt tgggtctttc ggcccagccc cagcgtggtt
    65881 ttgaagcggg accctggctg ggaggggcac attatttcgg cgatcttaat accacttata
    65941 aaattaacag gcccggtccg ctgggcagtc ttgccctgcg gtataatttt aatcagcggc
    66001 tcgccgccat ggccaaattg aacgctttcc gcgtgtcggc ggacgacgct ttatccacca
    66061 atgcctttga aaaaaaccgc aatctcagtt ttttctctac cgtttttgaa ggcagcgccc
    66121 agtttgaatt taattttttc ccctataaac acggtgataa ggaacgttac ttcaccccct
    66181 atctctttgc aggagcatcc gccttcaact acaatcccaa aacgaggtac aacggagccg
    66241 tcgttaaatt gcagcccctc ggtacggaag gccagcccat cggtgaggaa tacagcctct
    66301 ttcaacccgc cctgctctat ggcatgggat tcaaatacga tctcagtccg tcctggagcc
    66361 tcaatatcga aatcagcgcc cggaccttat acaccgatta tatcgatgat gtgagcaccg
    66421 tctatccttc accggatcaa ctggcaaacc gggacccgct ttccgccatc ctgagcgacc
    66481 gcagcctaca agcggacacg gaacccggaa gacagcgggg cgaccggagg gagagcgacg
    66541 gctttggcat gctcagtatc ggcctcatgt atttttttaa tcccctgccc tgtccgaaga
    66601 tccttaagtg atgcggaatt gtggaattgt aaaattgtgg aattgttttg tgtctacatt
    66661 tttataactg tgtaaccaca acctactaac cactatccac cgcccactat ccacgcccac
    66721 tgcccacccc tttgcttttc aaacggattg tctctatctt tcacgcttac cgataaccga
    66781 tagacgatca tgcgccagat tataactctt ttatattttt tgaccgctct ttccctccac
    66841 gcccaggtcc atatccggac caacctggtc ggataccagc ctgcagacca taaatccgcc
    66901 atcgcgttca cagacctgga cctgtccggg gaattccagg tcgtggatgt ttccggtaaa
    66961 cttgtttttt caggtccgat ccttcctgcc aaagcccccg gctacgggcc ttttaaaaat
    67021 tattatgaat tggatttttc cgggctgact gcaggcgggc aataccagct ggtgattccc
    67081 ggccgcttgc aggcgacctc accctccttc cacattgatc ccggcttata taaaaatctc
    67141 cccgacgtgt tgcttgaatt catgcggcag cagcgatgcg gatacaatcc attttttgac
    67201 cacgtctgtc accagaagga cggccgcatc atggatggac ccataccgga ctcaacctat
    67261 gtcgatgtga ccggaggctg gcacgatgcc ggggaccagc tcaaatacct gatcaccggg
    67321 agcaatgcca ccgcccgcat gatcatggct taccagcatt ttccccaggt gttcaaggac
    67381 caatttaacg ccctgggcca gcccggcgcc aataagatcc cggatgtgct ggatgaagcc
    67441 aaatggggac tggactggat tctccgtttg cattacgccc ccggccacct gatccatatg
    67501 gtggcggacg accgcgatca tgtaggctgg aaactgccaa tgtacgaccc ctccaatttt
    67561 ggctggggca aggattctta ccgggttgcc tattatgcca cgggcaaacc ccagggactt
    67621 ggcaaatata aaagccaggc tacgggcatt gcaaatgtgg ccgggcggtc ggccgctgcc
    67681 ctggccctgg gtcaccaggt ctggaaacaa aagggttatg acccggcctt tgccctcaaa
    67741 tgcctgcagg ccgcggaaga tttgtaccgc atggccaaac aacaagccgg ataccaacag
    67801 ggcaattcct atggggcgcc ataccggtac aacgaggata cctggacgga cgatatggaa
    67861 tgggccgccg ctgaattgta ccgggccacc cgcaagccct cttatctcac cgatgccaaa
    67921 acgtatgcca cccaaaccgg ggaactgtcc tggatccagc acgatacctc cgcccactac
    67981 cagttttatc cttttatgaa tattgcgcat tatgccctgt acaacctggt ggatccggct
    68041 tttaaagcca aactggcagg ctactaccga aacgggataa aacaaacact gaaacaacgg
    68101 gccggactca acgccttcca gcatggagta ccctttatct ggtgttccaa caacctgctc
    68161 atcgacctgg tgctgcaaat ccatctttat gaaaaaatga ccggggacaa acaattcagc
    68221 gcatacaaat taaacatgcg ggactggctc ttcggccgca atccctgggg cagcagcatg
    68281 tttatgaata tccccacgca gggtccgtac ccgcgtgatg tccatacctc cacctgggcc
    68341 ctgaccaaaa aggaggtccc gggcggactg gtggacggac cgatttatta taccacttac
    68401 caaaaacaaa tcggcctcca actcactcat gcggatccct atgccgcctt tcaaaacggt
    68461 tacgtagtct atcacgacga tatcggtgac tactgtacca atgaaccgac catggacggc
    68521 actgcctgcg ccgttttttt catggccctg tttacggaat gatcttaggg gtgaggagtg
    68581 aggtttgagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    68641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgttgacca
    68701 acttatgtca tggattctca taatttcctg actttttggc aggatactga taagggaata
    68761 ttatttgatg cttctatttg aatcgactaa tacgttttaa gtaataacca tgtttgactc
    68821 gttaaaattt tcagctcctg aagaaattga attccccctg ttggcgaccg aagaagaaga
    68881 ggagccttca agggaaaccg atgaaccgga gattttggga gtgcttcccc tgaaaaacac
    68941 cgtgcagttt ccgggtattg tactgcccat caccataggc cgggacaagt ccatacaggc
    69001 tgtgaatgaa gcctataaac acgacaagcg catagcggtc gtcacgcaaa aggaagccgg
    69061 caatgaggac cccaatttta acggcctata ccggatcggg accatcgccc gcatcctcaa
    69121 agtgctgaaa atgccggacg gaacgaccac ggccattctc cagggacgca aaaggataaa
    69181 aatcctcgag gagctgacgg aggagcccat catgaaagca agggtggccg ccctggtgta
    69241 caaacccgtg agcaacaagc ttgaatttga agccctcatc gccaccatca aggatctggg
    69301 caaacgcatc atcgaccttt cgccgcatat cccgggagaa gccgccacca tgctccgaaa
    69361 tatcagcgac gacagtttcc tgctcaattt tatagcttcc aacctgggcg tcaacctcca
    69421 gcaaaggcag caattgctgg aaatgaacga cctgcttaaa aaaggacagg ccatcctgga
    69481 gtacatgcac aaagacctcc agctgctcga gctcaaagaa cagatagaaa gcaaggtgag
    69541 gggggatatc gaaaaacaac aaagggatta tttcctcaat cagcaactca aaacgatcca
    69601 ggatgaactg ggccaaaatc cgcaggaaga cgagcttaaa aaactggaag tacgcgccgc
    69661 cgcaaaaaaa tggcccaaag cagctaaaga tgcttttgac cgcgaactgg ccaaactgcg
    69721 caggtccaat ccccagatgg ccgagtatgg ggtaaccctg aattatgtgg aatttctcct
    69781 ggatctgccc tggcaggagt acaccaagga taattttgac ctcaataaag tcaaggccgt
    69841 cctggaccag gatcattttg gactggatga tgtgaaggaa agaatcctgg agcacctggc
    69901 cgttctcaaa ctcaaggggg atatgaaatc gcccatcctt tgcctggtag gccctcccgg
    69961 agtgggcaaa acgtcactgg gcaagtccgt ttccaaagcc ctcaagcgga aattcgtgag
    70021 aatgtccctg ggagggcttc acgatgaagc cgagatccgg ggtcaccgca aaacttatat
    70081 cggcgccctt cccggccgga tcctgcagtc cattaaaaaa tccgcctctt ccaacccggt
    70141 attcatcctc gacgaaatcg acaaactggg caaggacttt cgcggcgacc cttcatcggc
    70201 tctcctggaa gtattggacc cggaacaaaa cacgactttt tacgacaatt atctcgagct
    70261 cgaatacgac ctgtccaagg tattattcat cgccaccgcc aattccctgc aaaacatcca
    70321 gcccgccctg ctcgaccgga tggagatcat tgagctcagc ggttattcga ctgaagaaaa
    70381 aattgagatt gccaaaaggc acctgatccc caaacagctt gcagcccacg gcctcaaacc
    70441 caaacacatc aagtggaacg acgaggtgat tgcccaggtg atcgacaatt atacccggga
    70501 atccggcgtc cgggccctgg atcgccagat cgcaggactg atgcgccggg tggccaagga
    70561 ggtagccctg aaatcctcct attccatcac cctcaccaag gcggatgtga cccggatgct
    70621 cggcctggaa aaggtcaacc cggataccta tcaggatgtg accatgcccg gcgttgccat
    70681 aggactggcc tggacccgag tgggcggaga catcctctac atcgagtcca gcctgagcaa
    70741 ggggaaagga aaactcaccc tcaccggaaa cctgggggat gtcatgaagg aatcggcgac
    70801 cacggccctc agcttcgtta aatccaaaag caagtccctg ggcatcgatg aaaacatttt
    70861 cgaaacgcac gatatccata tccacattcc ggaaggcgcc atacccaagg acgggccttc
    70921 ggccgggatt acgatgctga ccagcctggt ttccgccttt accaatacgc cggtcaaggc
    70981 ccacctggcc atgagcgggg aaatcactct tcgcggaaaa gtactgccgg tcggcgggat
    71041 caaggaaaaa gtgctggctg ccaaaagggc cggcatcaaa gaaatcattc tctgccggga
    71101 aaatgaaaaa gacatccggg aaatcaaacc cgagtacatc gccggattgc agtttaaata
    71161 tgtagaccgg atggaggagg tgattggttt ggcgatatag tgaggattga ggattgagga
    71221 ttgaggattg aggttagtcc ttggtgttag aggccgattg gaatactgac cagcagagta
    71281 tcccagccaa ggtacatgat cgcattttta tcatccacct tttcaaatac gattgaaaaa
    71341 ttttcgagtg ttttggtatt tgtcctcacc ggtactttaa tccgcaacac atcctgttct
    71401 gccttataac tataagcacc ccagtgatcc aaagcggagt tgaggatgat cgtccattca
    71461 ttggtttcag gaatagtaaa cagggcatag gtacctgctt ttaattccat cccgccaaat
    71521 agcagatccc ggtagaattt tatttcagtg gcttcgttgg ctcctgttcg ccatacttta
    71581 ccatacggaa tcagtttacc taaaagttcc ctgccgtttt ttgcaggccg gctgtaaatc
    71641 acccggatca tggccgtatc cgaacctatg cgttggggtg caaatcgccg gtcgtgggcg
    71701 taatcgttcg gataatagac catatccagg gggcttttat ccagctcgaa aaaattttgc
    71761 gcagctaatc cggaaaaatt cagggcgacc aggataacga tgaaaaggtt cttcatgcct
    71821 taaagataaa aattagcagt ggcatggttt gatcaccaaa ggatggtttc gccctgccag
    71881 tcggggaaaa gaggccggcg ctcaccggca tggttagata ccggacgaat tgatttataa
    71941 gtgatcgaac ataaagttta tgaagttcaa ccaggggggt gtctaaaaag tacaaaattc
    72001 acatgaatgg ccaaacaaag gcgaggttgc ttcagtgcct gcccgactga tcaataagat
    72061 aa
//
LOCUS       DAOQXT010000011        71332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_385_length_71332_cov_7.203684, whole genome shotgun sequence.
ACCESSION   DAOQXT010000011 DAOQXT010000000
VERSION     DAOQXT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 71332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71332
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_385_length_71332_cov_7.203684"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2746
                     /locus_tag="PKM27_02990"
     CDS             <1..2746
                     /locus_tag="PKM27_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNR06254.1"
                     /translation="MSIIKTPDQRVRVFISSTINELADERKAAREAIGNLRLIPVFFE
                     AGARPHPPRDLYSAYLDQSHIFLGIYWNSYGWVAPGAKISGLEDEYRLCGNNKPKLIY
                     VKRSSERQSRLQELLADIEKSETACYQTFSDADELQRLIENDLSVLLSESFENALSAR
                     QPKGGSDGDWSLAPHHRFEEIPNLRSEIYGREEDLARVNELLIKPGVGLLTLLGAGGT
                     GKTTLSVHVAHAVKDRFRNGAIFVPLAPVTDHRLVGATLAGVMGIQDSGKQPIEQTLV
                     DFLSDKQILMVLDNFEQVVEASRFLSDILGQCKDVKILVTSRTSLHIRNERIYNLAPL
                     VLPGEHQILSPEELRKYPATLLFVERALEVNQGLRLNKENTEAILEICRRLDGLPLAI
                     ELAAARTKFFQPAAMMARIEKSLDLVSKGQKDLPERQQTLRAAIEWSYGLLAEDTRKA
                     FRQLGIFKRSWTLDAMDVVLNGEKPTVDVEEMTERLLDVSLIKPSMAGHASEPRFNML
                     QTVHEYAMEMLEQAPEALETKKRYADYFLDLSKAASEYAWWAHGESWLDKLETEYQNI
                     RSAFYFFIEQKEYEKAWDLFYYMVVYWNIRGGFSEGLKWIEDAGILNPELQESELISV
                     ESRGRTLTWAALVRLMLMQIDEGYQLLHKAEEMLAPTDNRRDYAYALLFDGCFGAFLG
                     FPDAPVKIEQARVLCEGLKEPLPLTMFYVWGYEYYRQQNRMDLVNKHFEQAQKIASEL
                     GITYILGALFIQKFSLDVMNPENDWEQTAREAQSMYKLLPEKGYKGLKSAAMNAYAYA
                     QLMQGKPEASHLPLMKALEFARECGEKESEFYGIMLAIGYFKVVQNNEKAYKLFGALD
                     TFLEVTQYPLIGASEVQYAQVKSKLEPNFTLAENNRWYDEGRKWRIEEAVMYAMQEQT
                     "
     gene            complement(2772..4268)
                     /locus_tag="PKM27_02995"
     CDS             complement(2772..4268)
                     /locus_tag="PKM27_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter TolR"
                     /protein_id="HNR06255.1"
                     /translation="MKKSISVILLLFSIAGFGQMNPIGQFDHHQDVGNPKLPGSAVYD
                     EKEQTYTVTGAGINMWAKADQFHFLWKKIKGDFTISATIKFIGKGAAEHRKIGIIARD
                     ELTTNSRYADACVHGDILSSLQYRPTDTSNTYQVVLSSYHPTDIEFSRRGNLFTFSAG
                     TFGENYKSVSKEIELNEEVYAGLFICSHVEDVLETAVFSNVRIIIPVNPDYRPYRDYI
                     GSHLEVMEVATGHRKILYSVPNSIQAPNWTKDGKHLIYNSSAGLLYKYALADGKITEL
                     NTGEARSNNNDHVISFDGTKLAISNHVGERRISTLYTLPITGSDKPVQITSPDSGHSY
                     LHSWSPDGRKLIFTGQRKGQYDIWAVDIATRKETQITNSPTLDDGPEYSPNGQWIYFN
                     SVRTGTMKLWRMKPDGSGQQQVTFDEYNDWFPHFSPDGKQILFISFPKEIDPTTHPFY
                     QRVYLRIMPASGGVPRTIAYIYGGQGTINVPSWSPDGKKVAFVSNSKL"
     gene            4518..4901
                     /locus_tag="PKM27_03000"
     CDS             4518..4901
                     /locus_tag="PKM27_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR06256.1"
                     /translation="MTSETNSINWFEISVSDLPRARKFYETLFGIEMPTDTMMGMEMA
                     FFPYENGSGKASGALVKSDYHIPSENGVKIYLNGNPDLQNALSKVEGAGGRITMPKTH
                     IGDDVGYMAFFLDTEGNSIALHSNG"
     gene            5059..5373
                     /locus_tag="PKM27_03005"
     CDS             5059..5373
                     /locus_tag="PKM27_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06257.1"
                     /translation="MSQFQTLEDFKSSLSLAEPPKEMSLHLKALWYDANEDWDSAHKA
                     IQDEGDAFSARIHAYLHRKEGDLGNADYWYRRSGSSRPRLSLEEEWMELVRHGVQIDT
                     GH"
     gene            5817..8225
                     /locus_tag="PKM27_03010"
     CDS             5817..8225
                     /locus_tag="PKM27_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNR06258.1"
                     /translation="MAFAQESIQIKMQVMDENQRPLEGANVLIYRLPDSVFVKGDITN
                     ETGEAKELTGFKSGPYFSKVSMLGYSPSVSGSFTLQAGSAVHDLGIIQLKDQAVNLST
                     VTVTAQKPFIERRLDRLIVNVDGSIVGAGSSAFDVLERSPGILIDANDNISMRGRSGV
                     IIYVDGKPSPMSGADLANFLRGLPANAIDRIDLITNPSARYDAAGNAGIIDIKMKKDQ
                     RLGTNGTFSAGYGQGVYPKANTGLSLNHRNKKINVFGNYNYAYRLGLNHLLLDRNFYD
                     NFEFSGKDEKDNYTKIPGHFHALRWGADFSPNEKTILGVVLNANFNAFNPFNRNSSRV
                     FDQNRNPVFNFNTNTDNHNQNNNLIANLNFKRSLDSTGREITADIDYGRFSTLGTSSN
                     TTRYFQLNGEVLRPDYTLLGDQDGLLDFITAKADYTHPINKTTKWEAGFKTSFVSSDQ
                     DAKFWEKNGQEVTVDETKTNRFYYKEGNNAGYLNWSKEFKKFSLLVGLRGEHTQINTR
                     QEKGNIRFDSSYFQLFPSAFLNYRLDKNQTFSLSVSRRIDRPGYNSLNPFLFLIDVTT
                     YATGNPVLLPQFTWNFEAGYTRKTVNLTLAYSRTLQNQTVILSRFKDVFPHIPSEENV
                     TVQIPVNLISSDYYGLTMSTPLAVKSWWNMINNYSLFYNHFNGNLSGTSLNNGKAVFS
                     GSTNNNFTMKKGWAAELSFNFNTGNQYGYLTFEPQWALGAGIQKKVLKEQGTLRFNVT
                     DIFWTNLPKATIRFNNYIENWHAYRETRVANLNFTYRFGNTKVAQARRRTTGSEEERQ
                     RAGN"
     gene            8301..8738
                     /locus_tag="PKM27_03015"
     CDS             8301..8738
                     /locus_tag="PKM27_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR06259.1"
                     /translation="MKIKFLLFLLFIQAVPGFCQYQAKSKAEQSILDNFNEQVRCWNA
                     GDIECYCQGYLKSGETRIVSSRGITSGYEAILASYQKNWPKERMGQLHFDQLRMDKLS
                     SKAYLVHGRFNLTYPEAKALSGYFTVVMKKVGGKWLMFADHSS"
     gene            8986..10938
                     /locus_tag="PKM27_03020"
     CDS             8986..10938
                     /locus_tag="PKM27_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR06260.1"
                     /translation="MPERLMANNGALAIAYPIHQIKIDGDLSDWSWIKTSYPIAIHLS
                     DIKPRDASDFQAWFKIGYHLESRSLYIAVEVTDDDHIRDTAKNASWNSRDAVELDLDL
                     LHLPGRSGVTSYLLSVDHKNYNKSRWDPVTPAGWDKTEGAVIRQGNKSFYEWRVVLEG
                     PLSTPKSIGLDLQVFDKDAGGDFSYSAWGPEGYKYRNPNSLGDLILLKEGEEPHPLSG
                     RVKWAGEKKINFPATIGLNSEKEPGVFLDLALDSTGRYSASVPAGSYRMAVRNPYLSF
                     ENKWYVLSASPSPSIRVSPGARVESPDLELVYTPVPGLIPAKGILHDFNDLRAKELDA
                     FIETYQKQYQIPGVSLALIKDGKVIYSRAYGVKNSQTREKTDENTLYEAASVTKPVFA
                     YAVMRLVEKGKIDLDKPLYEYLPYPDIAHDARYKKITARHVLSHRTGFPNWRYMNADG
                     KLDIKFEPGSRYGYSGEGFEYLKKVVEKITGKKVEKILREEVLDPLGLYHTYFSKNDT
                     LAKLVATGHNDNIPSNDGLPEEPGMAYSMHTEAKAFTRFMLALLHQQGLKPETFREML
                     SLQSEYNTPEGQEKPKFREYMGISLAIRESPYGLVFGHGGNNGDFRCGFELYKDLKMG
                     YVIFTNANSALPLIEAMPKVLVEGKE"
     gene            complement(10968..12626)
                     /locus_tag="PKM27_03025"
     CDS             complement(10968..12626)
                     /locus_tag="PKM27_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNR06261.1"
                     /translation="MNTNKQTVHPPVIEPEDKNQDQAVPFPVFDPLDMNNYRVEKLKK
                     IPRKGFEKWMEQWGGLFAILAFVWIYFFADISFLNDIKPEILQKEGAQRLSEIGAEQF
                     SRINYAMLGVFVASIILWITEAVPNYLTSLLVILTMVLTGVTSENLAYAQLGHPVMWL
                     NILSFILASMLVTTQAAKRFALWFVLRFGKNAFWIFFSFIVINIVLSVFISATTAKAT
                     ILLPIFMVIAAIYGATPGKRNNFGRNIVLQNLFQINMGATGFITGSGANLLAGSLIAG
                     AMGKSIFGYQDWFVAAFPLAMILILAGWWLGTKIIFPMKPEEQRPQIEGGMARLKQEY
                     ESLGKMNRNEYKSIAIFLVVLALWATDKQHGISQTAVAFMGAVVALLPGIGVVKWNDV
                     DIPWHLMLFSAGAYALGTGLDSTGLPSSLVDALFRYFGISAETPFWVLYLSLTFIMLF
                     SGILFQAKTMRTLIFVPIAIGIAQKFNYPVMSLAFPVALLIEHVYVLPFNSKPAALLY
                     TTNQYSVIDSFKFGFTMMVISWIMIIIWGETVLRFLGHTVNGVF"
     gene            complement(12630..13118)
                     /locus_tag="PKM27_03030"
     CDS             complement(12630..13118)
                     /locus_tag="PKM27_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNR06262.1"
                     /translation="MAQEIEKKFLVTSDAYKAIALKKIRITQGYLSSVPERTVRVRIK
                     GESGFLTIKGIGNDSGASRYEWEKEIPLAEAQELMKICEPGVIDKTRYEVPAGTRRWE
                     VDEFHGDNEGLVVAELELANENDAFIKPGWIGNEVTGKVEYYNAMLMKTPYRRWPKSQ
                     RP"
     gene            complement(13129..13770)
                     /locus_tag="PKM27_03035"
     CDS             complement(13129..13770)
                     /locus_tag="PKM27_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06263.1"
                     /translation="MQTTVPSEGLGIKPRFEFRSFGQGFEIPAKRMARLSMPVPENIW
                     ERQSDEIYIVSRNNDINNTKIRDGMMDIKTLVQTLDGLEQWNPVMKGQFPLTAQILLT
                     EVFPAFGAALPELTKQAYSFDDFMELVRRHQDLQAVRVHKRRFAYLVNNTICETGQVL
                     INGARVETISSESTETADVHKTLKDLGLEGLENINYLQAIKRVIGMIDQPLAN"
     gene            complement(13770..14594)
                     /locus_tag="PKM27_03040"
     CDS             complement(13770..14594)
                     /locus_tag="PKM27_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNR06264.1"
                     /translation="MKRKYLIWSVFALLLFASSCVKDELFNGPATLSNVKQVPVAPNS
                     SEDVLVTARITDLKGIASVNLLYRVIAGGTFSSIPMTSTAQYEYQGTIPATPFGTKVE
                     YYIEVLNSGGFTSRYPEVENQFANYTVGASNLIKLYINEVFADGTKDETDPDWVEIYN
                     GSDIPVDISGYSFYDQGIKTGGKPKRVLNAGTIIPSKAFFVLKTEYTGGEYAIEFGLS
                     TSGDAVYLENAAGILVASLDFQTISLSGKKSYGRQPDGSDNLVVFTTPTRGTSNNH"
     gene            complement(14617..15975)
                     /locus_tag="PKM27_03045"
     CDS             complement(14617..15975)
                     /locus_tag="PKM27_03045"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNR06265.1"
                     /translation="MKTTAITAALAFLIQIAFAQDTSRQDRFNQYGQMVSRSPLDVEN
                     RNGILVFESADQDYRLWFDIRVQVDGHYFPDKTLNAIGNGTSIRRARFAVKTNLTRNW
                     YGELDLDFSNAVLELKDAYLMYDFRNGLAVRAGNFKERFSMLQNTSSRNVNFQERSMA
                     VEAFTPSRHIGLEGSYSGKYFMTAAGVFFQEVDDAELRTFVEDNNKDYGRDEGISLTE
                     KLVLQPFGDAADYGVHLAVAHSYRQPKTSVEVAEWGGLRYSTRSLSNVNRKKYLDTDI
                     IPDVKYSSINNFEFAAFHRGLVFQTEYLTSRVHRESDLAKLDFGGFYAQAGWLLFGGR
                     QYYNKLEGEFGQPYRGQKWGDVELTFRYDNIDMNDKDIYGGAAEAYTAGINFYAAKNI
                     RLMMNYSYVNHDRYASGKGKLFVGHDLTGALTKDPRQVADPKGKAGDDFGMLGVRFEI
                     NF"
     gene            complement(15992..17728)
                     /locus_tag="PKM27_03050"
     CDS             complement(15992..17728)
                     /locus_tag="PKM27_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNR06266.1"
                     /translation="MIFLFLASGLFLGWSLGANDAGNVFGTAVGTGMIKFRKAAIVAG
                     LFVILGATLQGAGATRTLSQLGSVNALGGAFTVALCAATVVAWMTYKNLPVSTSQAIV
                     GAIIGWCFFTTSPVDYTTLVVIMTSWLTGPVLGAVFAALLYLLMRTYIRKSRVHVIKL
                     DGRIRVGLLVAGAFGAYSLGANNIANVMGVFVNASNYTVQIGNLIIPSEQVLFFAGGL
                     AIAAGILTYSKRVMLTLGKGVLELTPETALVVVLSQALVLFLFSSSALSAFVVRMGLP
                     PIPLVPVSSTQVIVGSLIGIGMVKGVQEIRFKLLGSIFGGWLLTPFFAGGLTFISLYV
                     ISNVFEIPVMSRQQAVNAPQDLPVHFHFPAYPMVLWLFLALLVVALFAGGWYFRTAGQ
                     GVRSRYPLGQWDGNNGYRPLSGEFLGAEGIPGKAIIKPEPGLSDTNRNAVVKHALHLS
                     DERRFLESMRDSLEQAQREPELQKKNERIQGMLTALHQKMNDSAEIRRFYMELETAIG
                     PLPSKLTAAGSHLTVQEKKLALLLRLGLSSKDIAPLLGISHKSVDIARYRLRKKLDLR
                     KEDNLIQYINTV"
     gene            complement(17731..18375)
                     /locus_tag="PKM27_03055"
     CDS             complement(17731..18375)
                     /locus_tag="PKM27_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNR06267.1"
                     /translation="MFSSRSNKSIELIDGFFNGVDQSLLIFKEGVKNYLHQNTASFAN
                     NLQELAKLEAESDLIRRKIEETLYTQSLMPQLRGDIWRLLEELDNLIDLSKKNLFQFD
                     VENPLIPEELVLDFIKLTQLSLSAAEAVIPAAKSYFNQPAAVSEKIHRVYFYEKETDV
                     FADVVRRKIFKEMPALGLSEKFHLRYFTLHIETISDTAEKVADLLTIMAIKRIV"
     gene            complement(18575..19456)
                     /locus_tag="PKM27_03060"
     CDS             complement(18575..19456)
                     /locus_tag="PKM27_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HNR06268.1"
                     /translation="MKASRFWMTILPACLLFIQCKNNRNEDAIDMDRVFAWCIVPFDS
                     MQRTPQQRIEMLKELGIKKYAYDWRDRHLEEMVHEWQLARENLIEVSAVWLWLDERTD
                     TIGKLSPANERLFANLDSAGLHTDLWLSFNANYFDSLTQDQALAKGIAIVDYIASRAK
                     SLGCKLVLYNHGDWFGEPENQVQIIRALPGQDIGIVYSFHHVHHQLDRYNSLLDTMLP
                     YLKAVNLNGMRRNGPKILPLNEGDEEQAMIRTLLAKGYTGPLGILGHVEEADVKQVLE
                     KNKTGLEAILTGIAKRE"
     gene            complement(19485..20381)
                     /locus_tag="PKM27_03065"
     CDS             complement(19485..20381)
                     /locus_tag="PKM27_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR06269.1"
                     /translation="MSKLKRREFVKYSTLASASMVLPLNIPYGPGQPLTKKSLKYGMI
                     KEGATVLEKFKLIKDLGFDGVELDSPNPLQEKEILDARDKTGLEIPGVVNSLHWNSPL
                     SDPDPAVRGKCVESMKTSLRDCKKYGGTTVLLVPAVVNEKVSYAEAYERSQAEIRKIL
                     PFAEETGIKIALENVWNNFLLSPLEATRYIDEFNHPMIGWYFDVGNIVRYGWPEHWIR
                     TLGKRILKVDIKDYSRKKASDEGIWKGFDAELGEGDANWAAVNKALREVGYQGWGSAE
                     VNGGDRLRLKEISDRMDRCYAM"
     gene            complement(20453..21772)
                     /locus_tag="PKM27_03070"
     CDS             complement(20453..21772)
                     /locus_tag="PKM27_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06270.1"
                     /translation="MNKKLTNASRRNFIRLSGSTLAASTMGFNVIANPYPKHQFSAET
                     LKVGIIGCGGRGTGAALQAVRGDKNIVLTAMADVFEDKLEIAYNSLMEEAPDKIKVPA
                     NQKFLGFDAYKKVIESDVDIIISGTPPNFRPAHLEAAINAGKHVFFEKPVAVDAAGIR
                     KVIEVAKKAKEKKLGFMSGFCWRYDFPKRETFQRVLDGEIGDVLSIYNTYNTGAGGWR
                     EIKPSWGKFKKELRNWVYYTWMSGDHIVEQAVHSLDLMSWAKGDVLPIKASGTGGRQS
                     RIEPEFGNIFDHFAITYEYPDGSRGFHHSRQQKGTSGAYGIELLGTKGRCDINVWNRH
                     EIKGKQSWSWEKTHTEKDKNNMYQTEHDELMASIRKGDPINDGIRSAHSTMLAIWGRM
                     VAYTGKDLTWDEALNSQEMLTPHIDEYNWDLDWKIKEVARPGVTPFT"
     gene            complement(21867..23741)
                     /locus_tag="PKM27_03075"
     CDS             complement(21867..23741)
                     /locus_tag="PKM27_03075"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNR06271.1"
                     /translation="MMGIWKLMVVLAFLIPGSPAQTPVKPVVQDQLKAASSAQIHGYL
                     GRRLDSSYENRILAQDPDRLVAPFRKRTETSCWQSEFWGKWFTSAVLAYRYRPEARLK
                     TVLDKAVESLLATQSPDGYIGNYAPDRHLQSWDIWGRKYCMLGLLSYYDLTADKKALD
                     ATGRLANHLMMELKQKQVSIVRLGNHRGMAATSILEPICLLYARTGNKQYLDFAREIV
                     NQWESPDGPQLISKAGVDVASRFPKPQKNWYGWEQGQKAYEMMSCYEGLLELYRLTGE
                     AKYLQAVESTWDNIKETEINIAGSGSSVECWFHGREHQSEPVYHYQETCVTATWIRLS
                     QQLLRLTGQAKYADAIEQSYYNALLGALKPDGSDWAKYTPLSGQRLEGSEQCGMGLNC
                     CNASGPRALYTLPLTAVMEDESGIQVNFFTAGSYRLKTPNGKQIKVLQETDYPASGII
                     KLILEMEQPEEFSLKIRIPGWTSRMKFKVNEEDRMEGKAGSLFEIGRRWRNGDLVYLE
                     TDLRGRIHNSGAGHRHEAITWGPLVLARDGRLPGPSLESILTPVGDSTGYIQLEKVSP
                     SNQEIWMQFKAAFLPESYAEGGPEPIAVHLCDYASAGNSGEGLPFFKTWLPQFFDPRK
                     "
     gene            23817..25886
                     /locus_tag="PKM27_03080"
     CDS             23817..25886
                     /locus_tag="PKM27_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR06272.1"
                     /translation="MKKCILLLLSTLAPALKAQFSIQNVLSPPFPTELKASGNGHTLA
                     WVFNDRGERNIFVSESPFDQSRPVTSYTGDEGLELGQLNISTDGQRIAFVRGNAANSR
                     GEAANPAFLQVPTGQSIWIMDRDGSHLRNIGPGTSPSISPDGRQLVFLHRGEVWISDL
                     NDTAKPVQKLFQSRGTMAQLRWSPDGNKLAFVSHRGDHSFVGIYDFSIRSVYFVKAGV
                     DRDQYPAWSPDGGQLAYIRVPHINKIPPFTAVRTSNPWSIRVFDLGSGTDSEVWKADE
                     GRGSAFTNEIPVVENPLWWLDENQMLFPYEKTGWQLLYTLDIGKQSVSLLTPGKGEIE
                     NVCLSPDRKTLYYTTNIDDPERRHIWKMDLPDGKPFCITPGMGIEWSPVPISSGLAFL
                     QSSAKRPAWPAVLKGPARQDLAAERFPADFPSALVEPKLVKIKSTDGMMVSGDLFLPP
                     GYHPGLKYPALIFLHGGSRRQMLVGFHYSQYYSNAYALNQYFASRGYIVLSLNYRSGI
                     GYGLDFREALNYGAGGATEVRDLIAAGHFLRNRTDVIMNKISLWGGSYGGYLTAHGLS
                     QASQHFACGVDIHGVHNWNDEIPTFAPWYDAAKFPAIAKIALESSPVHYVKSWIRPVL
                     FIHGDDDRNVPFSETVNMIQQLRTQGVHTESLVLPDEVHGFLLHKSWLKVYEASFEFI
                     DRQMTGK"
     gene            25944..27269
                     /locus_tag="PKM27_03085"
     CDS             25944..27269
                     /locus_tag="PKM27_03085"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR06273.1"
                     /translation="MMKYFIALLIPLALHSQKHYSARLDQLMQAKYEVEGFSGTVLVM
                     HKGEKVYHKAFGLADREWNVPNTPDTKYRIGSVTKQFTAACILKLEEEGKLKVSDPLS
                     QYFPDYPYGDSITIHMLLNHSSGIKNYTSLPEFWPKAVLPLSHDSMVAIFKNKPLDFS
                     PGSRWAYSNSGYYLLGLIVEKASGQTFQDFLRDWIIEPAGLKNTGMDRLDSILTKRAK
                     GYGWKSPGGWRHADYISMEGPFSAGAMFSTVEDLYTWKKALMSNKILYPGSVAKMTTP
                     YLGAYGYGLSIDSLHTRPRIWHNGGIPGFSAHLAYYPQDDLCVVVISNNQSSADRAGI
                     GLASILFDKTVYIPYLPKETYLDPNLLDRYTGKFQASSPLEIRREGNALFRHTENGPP
                     IELKAESLTRLFYTDRSGRFLEFEFDPQGRVTRAWFIQNGEKTEMKKVD"
     gene            complement(27294..28133)
                     /locus_tag="PKM27_03090"
     CDS             complement(27294..28133)
                     /locus_tag="PKM27_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR06274.1"
                     /translation="MNIKLWGVRGSIPVSDPDTRQYGGHTSCVVVSQDGHFLVLDGGS
                     GMQKLNFGQELTGKRVDILLTHFHLDHLQGLGFFKSLFNPSMEVHIWGPASSTHSLHA
                     RLSRYLSPPLFPVLIRDLPCQLHLHEIANSEFEIGPFRIRSRYVIHPGPTVGFRISNG
                     NGAFAYIPDHEPALGLAGILDDPHWISGYDLAAGADLLYHDAQYTDEEYRYKQGWGHS
                     GLSDTMKFATLSGVKKLLLAHHDPGRTDVQIQDLLTELLLTGQYDFPFEMAGEGMEYK
                     IGT"
     gene            complement(28130..28525)
                     /locus_tag="PKM27_03095"
     CDS             complement(28130..28525)
                     /locus_tag="PKM27_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNR06275.1"
                     /translation="MPSYEIEIILSRQLADSLSMPVFITDTEGNLIYYNEPAEEILGK
                     RFEESGVMPVEEWGKIFKPVDEFKNPLSPEDLPLVKTLRNVYPYHKTFWIESLQGKTQ
                     KISVTSFPIIGRSGNFSGGIAIFWKDERL"
     gene            28628..29011
                     /locus_tag="PKM27_03100"
     CDS             28628..29011
                     /locus_tag="PKM27_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNR06276.1"
                     /translation="MRTLLFLIITLFVMPDVAFSQTASAGKVLRHVVLFKFKETSSAA
                     DIQKVETAFRNLPAKIKEIKDFEWGTNNSPENLNQGFTHCFQLSFASEADRAVYLPHP
                     DHKAFGKVLGPHLDKVLVVDYWAEK"
     gene            29197..29859
                     /locus_tag="PKM27_03105"
     CDS             29197..29859
                     /locus_tag="PKM27_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06277.1"
                     /translation="MKKYNIILLTLFLSRHAFAQPAVKDEPRHHLVFENEYLRILDVH
                     MAPNDTTQFHLHQTPSVFISLSKTLTTNQSLGQPVNPVRYDEIKAWYDPIPSPRIHRV
                     WNSDSSWLHIMDVELTAVRQKSNPPVIQSPFLQLLFNELQVTGYRLELPADNSIALPI
                     SDTGYLLVSLANSVIRFDMHGGSQKRMMKAGHYIWIPAGGASTLTNLDQSKAELVLLH
                     LK"
     gene            complement(29940..30836)
                     /locus_tag="PKM27_03110"
     CDS             complement(29940..30836)
                     /locus_tag="PKM27_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNR06278.1"
                     /translation="MKPRWIYYALALSLAAFLLLSWAGNRYALADVDPDQDEVRLRLM
                     GHEILKVIGDDSSRILPLRRLKGTEYILGFERVFSFVPDSIVRVVRQHLEGVSFSRAY
                     LFQVLDCGTSDIVYSFSVDPRDHQPVIPCEGRAAPEACYQMRVVFKEPPAAFKWYGFL
                     LFPLFISAGYFLQKRTNKPSGPAAHKPAEEQHTRSSPVVFLGRFRFNPSDQTLFFEED
                     GIPLSAKESELLAQFAQAPNQLIPRDQLMKTIWEDQGIIVGRSLDVFVSRLRKKLSVD
                     PEVKLIGVHGKGYKLLIPVEQA"
     gene            30968..32539
                     /locus_tag="PKM27_03115"
     CDS             30968..32539
                     /locus_tag="PKM27_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HNR06279.1"
                     /translation="MIKSFTTGSFPAGLVLVRVITGFLLIRVGWEITSVDKMSGYFQW
                     LTDIRFPLPVPMAYAGKIAELAGGVSFITGFLVRWTSIPVMITMAVITFIMGEGNLMS
                     DSFLLLLLAWVFFCTGAGKWSLDGFLPGLRPGKPIMAKSQILFFPALFFSLNAITGQP
                     VEPWIHKPFDQWPEIALVNRFSLKDGSGYDDPSLSYAGTAFLVRKDDAVFAVTVKHIL
                     FLAWRKFNNRVFISDKIGRWTMHLKNNPGLRLAAGPLLNEDPDEKIQDGFENGVLQRD
                     WLIFKTDSIPEKIHPLTIGSHLPSLGDKVYLTGNPYRIDHTVHFRGKVVKQEGPVFLV
                     QFEMPDTVFLGGASGSPVLDENGHLVGIFSTSRRDPASGRIQYIMNTTHYLHAVLENN
                     KPLNLNRRPLATLVDSLVRKHSVKTAMDQVKKMYRDPDAYFRYNLSWPDYKALDQTGR
                     RLILSGRTREANRYYRGLLDQYPGMHFFVLSMLDILVHAGKKEKALRFLEQAIQKADP
                     ETRTILEKKRNELTC"
     gene            32723..32878
                     /locus_tag="PKM27_03120"
     CDS             32723..32878
                     /locus_tag="PKM27_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06280.1"
                     /translation="MKSYTMMYYAFFFFSAFGAAAFGAFATGAGLTTAAATGLAAVFT
                     LCGRVLP"
     gene            33085..33705
                     /locus_tag="PKM27_03125"
     CDS             33085..33705
                     /locus_tag="PKM27_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR06281.1"
                     /translation="MSIADRKDREKKELTELILKEARALFLEEGFEKTSIRRIADRIE
                     YSPATIYLYFKDKNDLLLALHQESFTHLFEALSSVASVPDAFERLVALGKAYIEYGLK
                     NPEEYELMFMLSAPLEALECRHELWCDGQKAIDLVKMMVDDCKAHGYFRKDLDPDTTA
                     IMLWAQVHGLVSLYLKQRFKMFQDQDQKKLIDKTFGLFVKTLQCFR"
     gene            33745..35076
                     /locus_tag="PKM27_03130"
     CDS             33745..35076
                     /locus_tag="PKM27_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR06282.1"
                     /translation="MTFRNSAWILALIFLYPGLSAQAQQNALDDYIRQALEKHGLIKE
                     QDALIDEKKAGFMLANRQSGPELTLSTSYTLAAGGRDISFPLGDLLNPVSNALNDLTR
                     TQDFPQYENQNILFLPNNFYDLRTRISQPILRPEIKINRLIKQEQIGLQELEKEMVER
                     NIIRDVKKAYYQYIQAGDMISILRQALTVLDEGERVTQSLIRNGIALPSAILRIQAEQ
                     SKLATQVYAAENQRLNARAFFNYLTGRPDSDSIRAISLMALPDPASFTDSIRTEEINS
                     LEKGLAIQRLALDLENKFHAPKLGAQVDLGSQNFNFKWGGYLLAGLQLDVPLYDNKKN
                     KYRKEEILAGMKAASEKKDWAAKSLGLQLQQAKRQMASAVDQYHSYSPILNMSQRYYW
                     EILRRYKEGQATPAELVEAQAQITSAQLQQNIALYESWIRAAEIESLIKTQ"
     gene            35118..36167
                     /locus_tag="PKM27_03135"
     CDS             35118..36167
                     /locus_tag="PKM27_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR06283.1"
                     /translation="MKIHSFLLVLPLFFVFCGREKTEPAVQIHPDSLEIHKVKVAPVT
                     GTETNGIIRATGLLVSDLESRPAFKIGGVIEKTYFGEGDKVSKGALLARLNQSEISAQ
                     VRQARMAVEKTSRDFDRVNNLHADSVATLEQLQNAGTALDMAKESLRIAEFNQQFAEV
                     RSPVSGTIVKQLLRQGEIAGPGMPVYAIMGTGRQDWKIKSAVSDKEWARIKKGDPAEV
                     WFEAYPGLKLQGVVDQVAEMSNPGTATLDIEIRLPGQNQKLAAGLLCKVEIRAAARMG
                     SLKTIPIEALVSSNQGLGIIYVIEDGKAVRKTVRIDKILGDQVAVSSGLEKVGEVITA
                     GAFYLEEGHKVQIIQ"
     gene            36198..39257
                     /locus_tag="PKM27_03140"
     CDS             36198..39257
                     /locus_tag="PKM27_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR06284.1"
                     /translation="MNLTSFSVKNYQFTIIIFILALALGLNSLLNMPRGEDPPFGAPI
                     FVVLAVYPGTNPADMEQLVADPIEEALYNLGDVKRIVSTCSDGLMLCQIDFNYGVNVD
                     TKNNDVNREMNKIRANLPEGLLELRVERASSSDVVILQSALVSETATPRELNDLADEL
                     KRELERVKDLKWVKIQGEPVERIDIQLKLDRMATMGIGINQVIQAVNYNNINIPGGTI
                     DLGTKRFNIKTNSDFKTVDDIRNLIVHAGHEGQLVRLHEVADVYPILAEQNHIARQNG
                     KPAIWILSALKDKKNIINAREQMEGILKAYEARLPEQVQLVHAFDQSVSVQRRLGGLT
                     RDFSIAICLVLLTLLPLGMRASFVVLISIPLSLAIGLALLHLTGFTLNQLSIVGMVIA
                     LGLLVDDSIVVVENIERFMRQGYSRKEAAIVATKQIFIAVLGCTATLIFAFLPLAFLP
                     EGAGEFIRSMPMAVLLTILASLFVALTIIPFLSSLLLQKHERPEGNIFMRAFKRYVND
                     PYAGLLRWAFRHPVLTLLGTVVIFAGSLMLVPKIGFSLFPKSEKPMFTIDIKAASGSS
                     LKETNRIARRIEQDLIGTPGVVSVSTNVGKGNPRVYYNVFQQNYTPTYAQLLVQVPSS
                     MDVPAITAFTDSLRRNYEGVPDAEVEVKMFQQGTPVPAPIEFRLIGNNLDTLKHYSSI
                     LEEVIANTKGTIAIRNDIRTPKTDLRVNIDKAKAGMLGVLPGAVAQTVRLGLAGLETG
                     SLIEEDGKEKVIQISLGKPDARQALEVFDQLYVQSVSGAQVPLKQVASFSLEDSPLLI
                     QHINKERYTAVTSFVETGYNTIQLINDISQKTDQLPLPAGYRIVAAGEKESREESFGG
                     MGTIVLISVFFLFAILVLEFRTFKSTLIVLSVVPLGIVGALAALYIGNETLSFVATIG
                     IIALMGIEIKNSILLVDYTNHLREQGMELREAIMNGAETRFLPILLTSMTAIGGMIPL
                     VLENSPLISPLAMVLIGGLISSTLLSRIVTPLLYYLIPPAIVVKEEG"
     gene            39320..39649
                     /locus_tag="PKM27_03145"
     CDS             39320..39649
                     /locus_tag="PKM27_03145"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNR06285.1"
                     /translation="MSSHSYFDLVYEVTRHIPPGRVSTYGAIADFLSLGSARMVGWAL
                     NHCHGLKDVPAHRVVNSKGELSGRMHFKPPSSMAARLKKEGVKVKVDRVVDFERLFWH
                     PREMAVD"
     gene            39763..40323
                     /locus_tag="PKM27_03150"
     CDS             39763..40323
                     /locus_tag="PKM27_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06286.1"
                     /translation="MKKLLLFVMFLFCMAGLQAQREETLLRNMSFSGAWGAWNSSIGK
                     VADENVYFRGGYGGVEFGGDLFLGWGGYRSSDRFYAEGERMRMKWNGPMVSYAPHSYR
                     AVHPVFTLMLANGKLDPDNGPFDRIFVVQPSLGAELNLLRWCHVGAHGGYRFVNNVNL
                     NQFNDADFSGLYGELTLKFGFSWGRW"
     gene            40387..42192
                     /gene="mutL"
                     /locus_tag="PKM27_03155"
     CDS             40387..42192
                     /gene="mutL"
                     /locus_tag="PKM27_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNR06287.1"
                     /translation="MADIIKLLPDSIANQIAAGEVIQRPASVVKEILENAIDAGATRL
                     HLLIREAGKTLIQLIDNGSGMSETDARMAFERHATSKIRKAEDLQFIRTMGFRGEALA
                     SMAAVARVELKTRLQDRELGTRLQIAASVVENQQPVQAPVGTSLAISNLFYNVPARRK
                     FLKSDAVEFRNILDEFERIALAYPEIELSLHHNDQELYALPRTQLKQRIIGVFGKNIS
                     DKLLPIEENTDLVNIGGYIGTVDACRKKRGEQYLFVNRRFIRSSYLHHAVRAAYESLI
                     REEDHPFYVLFLDIDPARIDVNVHPSKHEIKFLDERIIYQYLRVACRHALGQYSEPVL
                     DFEQPDRGMVHTLSTSAPPPGLKATINQAHYSESAIQQDWRKIYEGIKPEQSGQALVL
                     GSNMSQAPAAPEEGADETFSPAPEKTPYQIHQSFIISPIASGFLLIDQEAAHQRILFD
                     QCLKKLESQKGDSQQILFPTTLQFSGSDKALLLELMPGLKALGFDLEEFGQNAFIVHG
                     LPVDWKETESIQAFIESLLRQTLEESDRKTNHRERLARHYARYQSIRAGQRLGAEEMQ
                     HLIDRLFACEDSAWSPFGGRCYTTFDLGEIRKKLK"
     gene            complement(42858..43439)
                     /locus_tag="PKM27_03160"
     CDS             complement(42858..43439)
                     /locus_tag="PKM27_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06288.1"
                     /translation="MEDKILVAHDTEELVSAPGLEDLYLKPIQKLFKQNRGRISKVKD
                     YAPVLMIDIKSEAGATLPALVQLIEPYLKMIHPVKRKNGVQIIISGNRGSPDTWLQWP
                     EWLFFDGRPNESYTLEVLQRVGLISDSYSKYKEAPGEILTVANRAHDQGKLLRLWGAP
                     DEAPSWDRLKTLGMDIINTDKVTECRRYFGGRE"
     gene            complement(43662..44411)
                     /locus_tag="PKM27_03165"
     CDS             complement(43662..44411)
                     /locus_tag="PKM27_03165"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNR06289.1"
                     /translation="MKKFQLLLLFCCFVSSMGLAQKQFRALLFTKTNGFHHESIHDGV
                     TAVKELADRHVFDVEWHEDPGRFTDNNLARYDVIIFLNTTGDILNESQQAAMEKFIRA
                     GKGFVGVHSASDTEYEWEWYTKMVGRMFKIHPPTQTAQLRFTTNKFPGLELFRESNPW
                     TDEWYEFGPEKTTGLNYILAVDENTYNTKVQWGQNKGEGMGFHPVAWYHEYDGGRAFY
                     TALGHVPAVYRDPAFLNHLYAGIYYAAKGKK"
     gene            44702..45814
                     /locus_tag="PKM27_03170"
     CDS             44702..45814
                     /locus_tag="PKM27_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNR06290.1"
                     /translation="MFSMFKNGFPLFRVVLALLSPFISLKSFAQDAVSLAQVYKDYFL
                     IGAAVTPQSLKTDEAGLILSQFNSLTAENAMKMGPIHPKENEYQWAGGDSIAAFAQRH
                     GLKMRGHTLCWHNQVAPWMFKTETGDTVTKEILMQRLKDHITTVVSRYKDQVYAWDVV
                     NEVISDSPGEFYRPSIWYRICGEEFITKAFEWAHAADPDALLFYNDYNETHPVKREKI
                     FQLVKTLKEAGVPIHGVGLQGHWSIYEPTAEVLEQTLDRFSQLGLQVQVTELDISVYA
                     KGQGRREPWPKDSLSVFTPQMESLQIERYKICFDLFRKYRHTLNSVTFWNISDRRSWL
                     DNFPVRGRKDYPLLFDKDLKPKKAFWPVADFQKQGG"
     assembly_gap    45887..45986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(46045..47037)
                     /locus_tag="PKM27_03175"
     CDS             complement(46045..47037)
                     /locus_tag="PKM27_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNR06291.1"
                     /translation="MPLKLVLVLPFALHIFSCKNKEEVSSSRIRVIFDTDANNELDDQ
                     HALAYLLFNQDRFEVEGVTVNATRSGGGIDQQYQEAERILRLCNEDSLPLLKGADASF
                     IEILPQLDSVTYDGKDAVEFISDRAREEPTKKLTIIAVGKLTTLALAFMKDPGLVSRI
                     RLVWLGSNYPEPGEYNLNNDTAALNFILNLPVDFEIVTVRYGKPSGTDAVKITQKEIN
                     EFMPGKGPKVKTPVTGRHGGTYRCFGDYSVSLFEHIDYHSDPPSRALFDMAAVAVVKD
                     STWAGKVVIPAPLLVDGQWVERPDNSRKITIWENFNKEAILQDFRESMAKGAKG"
     gene            complement(47099..47977)
                     /locus_tag="PKM27_03180"
     CDS             complement(47099..47977)
                     /locus_tag="PKM27_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR06292.1"
                     /translation="MPDLTEKNTVKSENQTSLFQDEGVFFDRWENHSDQAVSVPSFLR
                     KDRVHFYFGLEGHPRLHFGPYIRQIDEHSVTFLYNPLQDHAYVLELKPGVQLYTLFIS
                     LDKLHRLFSQDELPILHSEQIQQKFYQDKPMSQAILLTLSALARVQLNKSLEHTFYYA
                     KLLELVSLYFSDKESDNSSCPFLNDVNVLKKIKDAKEILIRDLKNPPTIENLAQQVSI
                     PEYQLKSGFKEIYGNTVYGYLLARKMEQARRLMDSSSPHVNEVAYALGYSNPSHFIAA
                     FKKHFGVTPKKYLMGK"
     gene            48126..50168
                     /locus_tag="PKM27_03185"
     CDS             48126..50168
                     /locus_tag="PKM27_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06293.1"
                     /translation="MIFRNPVFLLSLVSVCGLVRAQTVEDSFSIQNIPEIVVTGEFEP
                     LEAKRSVYQVRTIGQEAIRARGATRLQDVLTTETNIRFSQDLALGGSNLSMQGLAGQN
                     VKILIDGIPMVGRQGSSNEININQIDINSIQRIEIVEGPMSVIYGADALAGVINIITK
                     KAVEGRADLSLRIHEETVGKEYGWPQGLHNQSLNGGYKKGDWYFKGDLSRNAFGGWQG
                     TGMGRDLQWHPKTQYLAGGVAGLEKTKTTAHFRLDLMHEDVYNPGAAEAGQAIDQNYR
                     TTRWMNQVQWNHTLGYRFKLNGSVAFTHFQRKTQTTLFNEATGDRRLSLAPDSQDTTF
                     FRGFTTRLLLPWKINSAFSLLPGLDINFESGKGGRIEPGTHTIGDYGLILTGEWKGGE
                     KLQVRPGLRFTHNSAYQAPPLLPSLNLRWVFAKQQDLRLAYGRGFRAPSLRELYFSFF
                     DSNHSIVGNTELNAELSQSFTASWNWTVWQKETSQLTAGLTGFYNDVNNMIGYGQKPG
                     NALLISYINIDRFKTKGLTANMSWKTPTLHFTLNGTYTGRYNQAGLNEAILEEFTWSP
                     EIAGTLTYSLARWGTSFSLFHKYTGRTPYFEIISEGNEQKLRQAEVEAYSWTDLSLKK
                     ELKNFGSLTLGMRNLFNVININTATLNGTGVHSGGANRPVGYGRSFFATIHYTIIK"
     gene            50215..51606
                     /locus_tag="PKM27_03190"
     CDS             50215..51606
                     /locus_tag="PKM27_03190"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HNR06294.1"
                     /translation="MFLFVALQACKDDPALPDNLIGFESNQLGFTSTENEITLQVKFT
                     YPAYESGTVLINILAEGLVYDTDFTTEPATVSNALSIPFLAGETAKSIKVIKKPGLLV
                     DGDENIGFTLASAPAGTILGPNPSLKLTFAEIISASGAATVDGGGATYPNKVFIDLSA
                     NRQVAIPRTGWDLGFAAGNEFRVILNASNGMMAIATDKNSMAAVTAADTAGLSTRLSL
                     NAVFAAINLDPVPDWVPNAITWIDDPSGNLSKTALAEINTTDSENKVYIVNLGNGPGT
                     PAPELGWKKIRVLRKAAGYTLQHADIGSATFTEVSITKDNNFNFQYVDFEKGLVQVEA
                     QKDKWDISWTGFTNSTNFGSGPVPYYFQDIVLQNTHQVQTAQVLTSAIAYEAFSETNL
                     ATLDFGTQSQVKIGPNWRSGGGPGAAPAIRTDRFYIIKDAAGNVYKLRFTALTSDGER
                     GKPKYEYSLVKKG"
     gene            51745..53202
                     /locus_tag="PKM27_03195"
     CDS             51745..53202
                     /locus_tag="PKM27_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR06295.1"
                     /translation="MPVIKNAGLRIWILTVLWMSGPLLAAQDNLVPNGDFEEELPCPG
                     NLATLAHWFVPQNSVVDLNNLCPYIDWWRFIRDKKEGINGSQCGYAETYYRGFADDNI
                     YSGRIYLAAKLRKPLEAGKEYYFEMQFRAVDTFPNKKLVNTVFTNSQDIAFTKEPPLF
                     DFNIPRNYMELKPVVASPLYTDYEWHKLSHCFVADGTEHYLVIGNFKNDANTLTVSTG
                     KRNPNFPNGLIANYAIDEVVLTAMEIPLTDTAMCVGDTLSLNVGKSVPRALAYRWQDG
                     STRPVYPAYKQETVRVEMIYSAQCRRDKQIRVTPFSREFPVQDQFWDTVICEGQFTPV
                     LAGMHLLGENIRWEDGRSDPMRIIHDPGTYTADLSHRCFNSIRQVFKVEVESCLAGIY
                     LANLFTPNGDGKNDEFRPVFKPDFPAIQSYELSIYNRWGELVFISRNLETGWDGRRFN
                     QPVPPGVYLYSLRVTTQSRSGQRTHRKSGDVTLVY"
     gene            53307..53990
                     /locus_tag="PKM27_03200"
     CDS             53307..53990
                     /locus_tag="PKM27_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR06296.1"
                     /translation="MVSESLKEKIYKELSRGTRALSNPKRMELLDLLSNKAWSVEELS
                     KELSMSVANTSQHLQVLKSAMLVKTRREGNFRFYTISDDRVLRLVAAVKELGFSQYAE
                     VEKIINDFKADKNILDSFTLDDLIRRARTEKILLIDVRPKDEYLAGHIPEAVSFPLSK
                     LKKRIHELPKGKTIVAYCRGPLCVMAVEAVKILQGKKIKAVRMEDGYVEWKLKQLETI
                     GSGPAFKNG"
     gene            54058..54411
                     /locus_tag="PKM27_03205"
     CDS             54058..54411
                     /locus_tag="PKM27_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNR06297.1"
                     /translation="MKILIIINDPPYGTEKLYNAMRLTHQLVKDHPEIELRVFLMADA
                     ATAALTNQVTPNGYYNVERMLKLALSKGVKIKICGSCAEARGINKLPLIEGTELSNMS
                     ELAHWVVDSDKVLTF"
     gene            54570..55796
                     /locus_tag="PKM27_03210"
     CDS             54570..55796
                     /locus_tag="PKM27_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR06298.1"
                     /translation="MEHIRLGLKENWRQFTLLVIINGFVGGMVGLERSILPKIAEQEF
                     EIAAKTAILSFIVVFGIVKALTNYYTGVLANRFGRKKLLIAGWVAGLPIPFMLMFAPD
                     WNWVIAANILLGINQGLAWSSTVVMKIDLVGEKQRGFAMGLNEFAGYLSLALVAFITG
                     WIAGEYGLRPYPFYTGIVLVGLGLLASIFLVKDTRHHVDREKLTSTLPALRNVFWDTT
                     WRDKNLGSVTQAGLINNLNDGMAWGLLPILLAGKGFTLGEIGLVTAVYPAVWGLGQLI
                     TGAMADRFCKKNMLYLGMFLQSLALFFLVMAHTLTHYIFLSSLLGWGTALVYPTFLAT
                     VAENTNPEDRAKSIGIFRLWRDLGYAIGAMLTGIVADLVNINMSILLIGTLTFASGCI
                     LLFRMKCRTVIRSEIS"
     gene            complement(55813..56508)
                     /locus_tag="PKM27_03215"
     CDS             complement(55813..56508)
                     /locus_tag="PKM27_03215"
                     /inference="COORDINATES: protein motif:HMM:NF005841.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNR06299.1"
                     /translation="MQNIGAETVDDYLSAFPAEIRIRLEQIRKVILKAAPEAREGISF
                     YIPAYRYHGKLVRFAAYDRHIGFYPGAGAIQAFKTALAPYVCSKGAVHLPLHGPLPLK
                     LLREMILFRVKENMEKQDLKSMTSIAGKKKKTCPRGHVFNKSSDCPVCPVCASLARPR
                     EGFLSKISAPARRALEAKGILELSQLSKYTEQEILSLHGIGNKAMIELRLALEEKGLR
                     FKAAGGPVSAEKA"
     gene            complement(56514..56882)
                     /locus_tag="PKM27_03220"
     CDS             complement(56514..56882)
                     /locus_tag="PKM27_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNR06300.1"
                     /translation="MNSRPVTIDEYIAGFSGEVRVILNKIRSTVRDAAPEATETINYQ
                     IPTFTLNGNLVHFAAFKNHIGFYPTPSGMSAFAGELSAYKGAKGSVQFPLDQPIPYDL
                     IARIVRFRVVENKQKSGKKK"
     gene            complement(56981..57496)
                     /locus_tag="PKM27_03225"
     CDS             complement(56981..57496)
                     /locus_tag="PKM27_03225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR06301.1"
                     /translation="MEKRIKLRKASLEDLELLRYWDKQEHVLESDPNDDWNWEYELQR
                     EPEWREQLIAESDGKPVGFLQIIDPALEESHYWGEVPRGLRAIDIWLGEQADLGKGLG
                     TEMMKQTIARIFENAGINAILVDPLESNVRAHRFYERLGFRLLEKRRFGQDDCLVFRL
                     VRKDWDQDSGL"
     gene            complement(57577..58650)
                     /locus_tag="PKM27_03230"
     CDS             complement(57577..58650)
                     /locus_tag="PKM27_03230"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNR06302.1"
                     /translation="MQIFPFHAHIPDLKKVKFDDLFYQGIREDFYKAFRKGVFKPVPG
                     EAFFILEIKEGQTTSTGLIAMTDIRDYLNQHIRKHERTLDKKQKLHHQLFIERRAMIK
                     PAALMIPAHARLNDLYREVKSGSKPLLKIRFPDKTVIHTLWKVDDPRRISSLSRQYKQ
                     SVRTAVIADGHHRFATSALLYSRAGGWAHQRILTVYFTPDQLKISGFFRVVDPLKSSS
                     AQKIAQRLKQATEQWEPVQELLNKKDFLYMAHDHQLYRFRIRARAGRVALPLLFSEKI
                     LNEVFEIRDETRSPRISYFEKTYDVEVLKTLMEQYPQKYIFILPSLTPREILSNKKIL
                     PPKSTYFMPRIINGLVVALGQGL"
     gene            complement(58869..59972)
                     /gene="gcvT"
                     /locus_tag="PKM27_03235"
     CDS             complement(58869..59972)
                     /gene="gcvT"
                     /locus_tag="PKM27_03235"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNR06303.1"
                     /translation="MKELALAEIHKSLGARMGEFAGFNMPIYYLRGITEEHLQVRNKV
                     GVFDVSHMGEFIVKGKEALNLIQSLTTNDASKLEIGQAQYSCLPNLEGGIVDDLLVYR
                     LDEDQCAEGEQAFMLVVNAGNIDKDWAWINASNTFDTRLINISDQTALLAVQGPLAAR
                     ALQPLTSISLEEMAYYTFTKGQFAGVDNVLISATGYTGAGGFELYFDEKDAIPLWQAI
                     FDSGKSLDIQPVGLGARDTLRLEKGYCLYGHDIDDTTSPLEAGLGWITKLSKGPFSSS
                     EIFAQQKKDGVKKKLVGFKVNDRRVPRQHYSILDSEGLPIGTVTSGTISPSLGYPIGM
                     GYVETSCSTPGSLIRIKAGERMLDAEVVKLPFL"
     gene            complement(59969..61978)
                     /locus_tag="PKM27_03240"
     CDS             complement(59969..61978)
                     /locus_tag="PKM27_03240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR06304.1"
                     /translation="MKFLLKNLLVFAFFASSCVPVKQEGAASDAIDITPVELDLRDSV
                     FASVLKAQNDQDLVVLASYFSAPGPSIRYAAALALGSVRDSSMADTLAHLLHDANVHV
                     ASAAAYSLGLMGATHSQDALLNAFRQYDTSGINSPINSSILEAVGRLGTPDFLQAMAT
                     VETYEPTDTLLLLGQMRGIYRYMLRGMTDPAGTATALKYLADKSYPGEVRMLAAHYLS
                     RAKDLDLSGQADQLLALLQGESVQEIRLPLVLALGKTKSEGAWNALVEIIRTDTDTRM
                     VCNALRAMNLYDYSQTKNIMLAALRNKDISIARTALEYLDNFGRVADAGDYRNIAREN
                     LPWQVKVPLLGIANENYSYAYGITKGNINTELKSILSRAAGIDEKTACLRALATDPKN
                     YSFILETGRQSALPAIHSAVMEAGIEILKSKYFSSAFGNGEGVKKEILAYITGHLSTG
                     DEAALEIVESLARDGSSGLKTLVDIPTLEQIKSSLAANPYAQYMLGKTLNSLKGTASP
                     LARMDNYKKLSLTDFTSSAAPAFADIHTDKGTIRLRLLNALAPATVINFVNLARNEFY
                     NGKTFHRIVPNFVIQGGCPRGDGFGSMEYSIRTETPPVYYNKAGLVGMASSGPHTESC
                     QFFITHSPTPHLDGNYTVFAEVVSGMDVVNQVQPGDKIQRINITE"
     gene            complement(62157..62369)
                     /locus_tag="PKM27_03245"
     CDS             complement(62157..62369)
                     /locus_tag="PKM27_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06305.1"
                     /translation="MAKNITLKNQLVSTVIGFGASPDPFNKTAEEDQQVREFLNQIKN
                     LRIRPSLSGIRKITEYARMQTSAPHS"
     gene            complement(62496..62933)
                     /locus_tag="PKM27_03250"
     CDS             complement(62496..62933)
                     /locus_tag="PKM27_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNR06306.1"
                     /translation="MNNVIIAFVILLFSMSWLPVRAQTMTGTWRTVDDRDKQSKSYVR
                     IYEETGLLFGKITKLLKDKPDAICEVCKGPRKNQALLGMVVIEGLRKIRDQYGDGRIY
                     DPVTGNDYKCTIWIEAGKPDELKVRGTHWSGIYRTQTWYKVTD"
     gene            complement(62959..65817)
                     /locus_tag="PKM27_03255"
     CDS             complement(62959..65817)
                     /locus_tag="PKM27_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR06307.1"
                     /translation="MPFKQFLIGLLSLFCLPDSLVLAQLLEWAPAFPKAEDTLRIVMD
                     ATKGNKGLLDYNGKVYVHIGVITSASSSSSDWKYVPFTWGSEMAGSQAVNLGKNRWQY
                     SIPGIRSFFKVPSSEKITHIAILFRAGGCSNCAAQRNRDGSDMYIPVYDDKLATRFIK
                     PAFQPLYKPIPEIIEVFPGQSLPIEAVSNAEAEWELFLNQQKVGQYPARDTLAVNLDI
                     PAGGSYTVTVAAHSANQAARDSFAFFLADSIVKKPLPEGVKPGINYEEGDTSLILVLV
                     APFKNRVAVIGDFPGSNWSEKQAFQMNKADDGRTWWLRITGLSPGVEYAFQYLVDGSL
                     KITDPYTEKILDPVHDPFLSAETYPGLKAYPTGQTTGIVGVVETRRPVYAWQNTFNRP
                     EKTNLIIYELLLRDFLAKHDWKTLTDTLNYLSRLGVNAIELMPVQEFDGNQSWGYNPS
                     FFFAPDKYYGPENDLRQFIDSCHGRGIAVILDLVLNHATGACPLAALYWDGINQRPSS
                     DNPWFNVTATHPFNVFNDFNHQSPDTRYFSLRVMEHWLKNFNIDGYRFDLSKGFTQVK
                     NPNDVGRWSDYDPSRVAIWRLYHDSIQQYSPGAYTILEHFADNGEESALANYGMLLWG
                     NANYAYGAATRGIHEASTFDWSVYQTRGWSQPHLVSYMESHDEERLMYLNLQSGLSSG
                     TYNIRDFNTAVERNKMAAAFFLLTPGPKLIWQFGELAYDFSINTCQNGMIDNNCRLDN
                     KPPGWPLLQNAKRKELYDWYKRLIEIRNFPWFKGKFSQASLQYRLNSPMKYLSLQSDS
                     ISLVVAGNFDIQPVTASVRFPRTGTWYDLKDSSSISVETLFTDLNLMPGEYHVYTDRK
                     LDVTTPVKYVDAGIHALRVLPNPSRDLAFLEISAGREDRMEIGIFGVDGKPLPWKHQL
                     HVIPGTQVIELPSLVSPGMYLIRVAGARGVKTVKWSVN"
     gene            65900..67369
                     /locus_tag="PKM27_03260"
     CDS             65900..67369
                     /locus_tag="PKM27_03260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNR06308.1"
                     /translation="MKRLNLALTLAILGLIFYQCSRNPVTGKKQLNLMSEEQEKAMGA
                     ESNPAVLAEFGKYEDANLQAFINEKGQQMAAISHRPQHGYTFQVISSPVVNAFAVPGG
                     YVYFTRGIMAHFNNEAEFAGVLGHEIGHITARHANAQAKDQLLTQIGVIGLIIASPAA
                     AQMAETLMQGAQLLLLKFSRAHETQSDELGVEYSTKIGYDAHQMAEFFKTLARLSDQA
                     GARIPSFLSTHPDPGDRYNRVHKLAEAAQASINKSTLKVNRDEYLRRIHGLMYGEDPN
                     EGYVEGNVFYHPQLRFRFPIPTGWQTQNSPVQFVMAEPNGKAMMLLVLGQGANFDEVI
                     ANSNKQYGLAPIRSENRKVNGYNTVVVLATQQVAQGQQAPPEAEKPMILSYYIQKDQY
                     IYCLHGVCANKDFQGYQNLFLNTMEGFSNLTEASKINVQAEYIYTVNAPSQSTLSALL
                     TRYGIEQKRLNEFAILNGMELTETVPAGKLIKIAKKGKA"
     gene            67382..67636
                     /locus_tag="PKM27_03265"
     CDS             67382..67636
                     /locus_tag="PKM27_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06309.1"
                     /translation="MMSLADVLGALGVFLILLAYFFNLYKKIETGSLLYILMNLAGAI
                     LAFLSSVMIHSLPFMVLEGTWAVVSLVALIRLYLKPWRKT"
     gene            67700..68011
                     /locus_tag="PKM27_03270"
                     /pseudo
     CDS             67700..68011
                     /locus_tag="PKM27_03270"
                     /inference="COORDINATES: protein motif:HMM:NF023159.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
     gene            68202..69374
                     /locus_tag="PKM27_03275"
     CDS             68202..69374
                     /locus_tag="PKM27_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR06310.1"
                     /translation="MLYLSGIILSFFLCFLLFTKRNKTAADFILTAWLGIIGIHLLSF
                     YVLFTGQFVKYPTFVALGFSLPLTHGPFLYLYTRQQTSSDPFQAKELFHFLPLFLSYL
                     LFAGFFLMPLEQKIEVFRQAGKGYETQSRINIFAINISGIVYSGLSIARLLKYRKNLV
                     HQFSNTDKINFNWLLYLIIWIVLIWITIIFVQKDQWIFGMAALFVLWLGYFGIKQVRV
                     FSQNAPLLKTETSSTVQRNMEDKRLYGQEIQRETEALASEKESENLKYQKSTLSERDA
                     SEIHARLERLMAEQKPFKNPDLTLNELANSLDVHPNHLSQVINSRVKKTFYELINDRR
                     VEEFILLSGQPESQQFTLLSLAHDCGFNSKASFNRNFKKYTGLSPREYLKRQLTEA"
     gene            69531..70541
                     /locus_tag="PKM27_03280"
     CDS             69531..70541
                     /locus_tag="PKM27_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR06311.1"
                     /translation="MKNTSSLLALAFLALVSFSCSKERFLNDPGNLVPKTVDQDPGLP
                     SILVNGVLLHSEAFGHPDSTMIVCIHGGPGGDYRYLLNCKDLANYGYRVVFYDQRGSG
                     LSQRLPKASYTSLGLAALDAVFNELGGVIAHYRTNPNQKVILLGHSWGGILATGYAGK
                     NPGAIQGLIVGEPGGLKWAEISEYIKKSNAFNLWSESLNDATYTDQFVTGREDRHEIL
                     DYKLALLAGTNEITGENNTEPGSAWRSGAVIRDAFLEIGENYKPDFSEGIGQFKTPVL
                     FFFSEKNKAYPDSWAHKISGAYPLADLHKVAGVGHSGIFQDKKTWQEKTLPKLLTYLN
                     SL"
     gene            70578..71252
                     /locus_tag="PKM27_03285"
     CDS             70578..71252
                     /locus_tag="PKM27_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06312.1"
                     /translation="MKTYIKSILLSLLTPFVSTLDAQTLNWANLQEENRHIIHAQFGM
                     EYGAVFGIGYGYQMNTGLFPMVASMEYSFPSGKNLLDDLKSKIGVQIRLVQYRHFRFS
                     AKLQGVFRRHETGLVRLLNFGSDFSGILGIYRPKWFFAGEFGFDKAIVSHFKHSKAYR
                     DQHPQVVDGWYEPATGGNFYYGVQTGFSIKNQDIFLKGGKMFAQDFKTKPLLPFYGQL
                     GVNLKF"
BASE COUNT        17696 a        18234 c        17639 g        17663 t
ORIGIN      
        1 aatgtcgatc atcaaaactc cggatcagcg ggtcagggta tttatcagtt ctacgataaa
       61 cgaattggca gatgaacgca aagccgcccg ggaagccatc ggtaaccttc gtttaattcc
      121 cgtatttttt gaagcgggcg cccgccccca ccccccccgg gacctttatt ccgcctatct
      181 ggaccaaagc catattttcc tgggcatcta ctggaacagt tacggctggg tggcccccgg
      241 tgcaaagatt tccggtctgg aagatgagta ccgcctttgc gggaataata aaccgaaatt
      301 gatctatgtg aaacgttctt ccgaacggca atccagattg caggaattgc tggccgatat
      361 cgaaaaaagc gaaaccgcct gttaccagac tttctcggat gcggacgaat tgcaaagact
      421 cattgaaaac gacctctccg ttctcctttc tgaatctttc gaaaacgctt tgtcggcccg
      481 gcagccaaag ggagggagcg acggggactg gtccctcgcc ccgcatcatc ggtttgaaga
      541 aatccctaat ctccgttcgg agatttatgg ccgggaggag gacctggcca gggtaaatga
      601 actcctgatc aagccgggtg tcggcctgct tacccttctg ggcgccggag gtacgggtaa
      661 aaccaccctt tcggtacatg ttgcccacgc tgtaaaagac cgattccgga acggagcgat
      721 atttgtcccc ctggctccgg tcacggacca tcgcctggtc ggagctaccc ttgccggggt
      781 aatgggcatc caggacagcg gcaaacaacc cattgaacag accctggtgg acttcctgtc
      841 ggataaacaa atcctgatgg tcctggacaa tttcgaacag gtggtggaag cctcgcgatt
      901 tctgagtgat atcctcggcc aatgtaaaga cgtcaaaatc ctggtgacca gccggacttc
      961 ccttcatatc cggaatgaac gcatttacaa cctggccccc ctggttttac cgggcgaaca
     1021 ccagattcta agcccggagg aactgagaaa atatcccgcc accctgctgt ttgtcgagcg
     1081 ggcccttgag gtcaatcagg gactccggct taataaagaa aataccgaag ccatcctgga
     1141 gatttgcagg cggttggatg gtttacccct ggccatcgaa ctggctgcgg cccggacaaa
     1201 atttttccag cccgccgcca tgatggcccg catagaaaaa agcctggacc tcgtatccaa
     1261 aggccaaaag gacctgcccg aaaggcagca aaccctccgc gcggctatag aatggagtta
     1321 tggtctgctg gccgaggata cccgcaaggc atttcgccaa ttgggcattt ttaaaagaag
     1381 ctggaccctc gatgccatgg atgtcgtcct gaatggcgaa aagccaaccg tggatgtgga
     1441 agagatgacg gaacgcttat tggatgtcag cctgatcaaa ccatccatgg caggccacgc
     1501 ttcagaaccc cggttcaata tgctgcaaac ggtgcatgaa tatgcaatgg aaatgctgga
     1561 acaagctccg gaagcattgg aaactaaaaa acgctacgcg gattactttc tggatttaag
     1621 taaagcggcc tctgaatatg cctggtgggc tcatggagaa tcctggttgg acaagctgga
     1681 aacggaatat caaaacatcc gttctgcctt ctattttttt attgagcaaa aagaatatga
     1741 gaaggcatgg gatctgtttt attacatggt agtatactgg aatatccggg ggggattttc
     1801 ggaaggcctg aaatggattg aagacgccgg aattttaaat cctgagctcc aggaatccga
     1861 actcataagt gtcgagtccc ggggaagaac cctcacatgg gcagccctgg tgcgccttat
     1921 gctcatgcaa attgatgaag gataccaact gttgcataaa gcggaagaaa tgttagcacc
     1981 caccgataac cgcagggatt acgcctatgc cctactgttt gacggctgtt tcggtgcctt
     2041 cctgggcttt ccggatgccc cggttaaaat agaacaagcc agggtgcttt gcgaggggct
     2101 aaaggaaccc cttccgttga ccatgtttta tgtatggggc tatgaatact accggcaaca
     2161 aaaccggatg gacctggtaa ataaacactt tgaacaagcc caaaaaattg cctccgaact
     2221 cggaattacc tatatccttg gtgctttgtt tatacaaaag ttcagtttgg atgtcatgaa
     2281 tccggaaaac gattgggagc agacggcccg ggaggcccag tccatgtata agttattgcc
     2341 cgaaaaggga tataaaggac tcaaatcagc cgctatgaat gcttacgctt acgcccagct
     2401 tatgcaaggc aaaccggaag caagccacct tcccctgatg aaagcgctgg aatttgccag
     2461 ggaatgcgga gaaaaagaaa gcgagtttta tggcatcatg ctggccattg gatattttaa
     2521 agtcgtccaa aacaatgaaa aggcctacaa acttttcgga gcgttggaca ccttcctgga
     2581 agtaacccaa tacccgctga ttggcgcatc agaagtccaa tatgcccaag tcaaatctaa
     2641 acttgaaccc aattttacac tggccgaaaa caatcgatgg tacgatgaag ggcgaaaatg
     2701 gagaattgaa gaagccgtga tgtacgccat gcaggaacag acctgatcaa aaaccttagg
     2761 ctaaaggccc tttacaactt actgttgctt acaaaggcaa cctttttccc atccggcgac
     2821 cagctgggta cattgatggt cccctggcct ccgtatatgt aggcgatcgt tctgggcacc
     2881 ccgccacttg ccggcatgat cctcaggtac accctttggt aaaaaggatg ggtggtcgga
     2941 tcaatttctt tgggaaagga aataaaaagg atttgtttgc cgtcgggtga aaaatgggga
     3001 aaccaatcgt tgtactcatc aaaggtcacc tgctgctgac cgctgccatc gggtttcatc
     3061 cgccagagtt tcatggtgcc cgtgcggact gaattgaaat agatccactg gccgttgggg
     3121 ctgtactccg gcccgtcatc cagggtgggg ctgttggtga tctgggtttc tttccgggtg
     3181 gcaatgtcca cggcccagat atcatattgt ccttttcgtt ggccggtaaa gatgagtttg
     3241 cggccatcgg gagaccaact gtgcaggtaa gaatgaccgg aatccgggga agtgatctga
     3301 accggtttat cggaaccggt gatgggcaaa gtgtacaaag tggagatcct tctttccccc
     3361 acatgattgc tgatggccag tttggtccca tcgaaggaaa tcacatgatc gttattgttg
     3421 gaccgggcct cccccgtgtt cagttccgtg atctttccgt ccgccagggc gtacttgtac
     3481 aagagcccgg cggaactgtt gtaaatcaga tgtttcccgt ctttggtcca gttgggagcc
     3541 tggatggaat tgggtacgct gtataaaatt ttccggtgac cggtggccac ctccatcact
     3601 tccaggtggc tgccgatata atcccggtaa gggcggtaat ccgggttcac gggaatgatg
     3661 atccgcacat tgctgaatac cgcggtctcc agcacatcct ctacatgaga gcaaataaac
     3721 aggcctgcat agacttcttc attgagttcg atttcctttg aaaccgattt ataattctcc
     3781 ccgaaggtcc cggcagagaa agtaaacaga ttgcctctgc gcgaaaattc aatatccgtc
     3841 ggatgatagg aggacaggac gacctggtag gtgttggagg tatccgtggg ccggtattgc
     3901 agggaactga ggatatcccc gtgtacgcag gcgtcggcat accgggaatt agtggtcagt
     3961 tcatcccggg cgatgatgcc tattttgcgg tgctccgcgg cgcctttgcc aataaacttt
     4021 atggtggcgc tgatcgtaaa atcccctttg atttttttcc ataaaaaatg aaactggtcc
     4081 gccttggccc acatattgat tcctgctccc gtgacggtat acgtctgctc tttttcatcg
     4141 tacacggccg aacccggcag tttagggttt cccacgtcct ggtgatggtc aaactgcccg
     4201 atgggattca tttgcccgaa gccggcaatc gagaataaca aaaggattac agaaatgctt
     4261 ttcttcatga ttaaatgatt gggccactaa gttaggacgg gatctgcaaa gggggctata
     4321 ccgccttggg atttcggcct gaaatcgttg gcccatatca cattcttaat ccaaaatgaa
     4381 acaaaaccct caaaaaaggc gagtggtctt gagtggcctc aaataataat tttcacgggt
     4441 tttgatttgt tgtctaattt tggtcactcg ctgaatcacc gattcatttc ctttattaaa
     4501 gtttaaaacc ttattttatg acctcagaaa ccaacagcat caactggttt gagatttccg
     4561 tgagcgattt gccgcgtgcc cggaaatttt acgaaactct tttcggcata gaaatgccca
     4621 ccgacaccat gatgggcatg gaaatggcct ttttccccta tgaaaatgga agcgggaaag
     4681 catccggcgc attggtcaaa agcgactatc acattcccag tgaaaacggg gtaaaaattt
     4741 atctcaacgg taacccggac cttcaaaatg ccttgtctaa agtggaagga gcgggtggac
     4801 gaatcaccat gcccaaaacc catatagggg acgacgtcgg gtacatggcc ttctttttgg
     4861 atacggaagg gaattcgatt gccctgcatt cgaatggttg aattgtggaa ttgttgaagt
     4921 gcggaattgt tttaaggcta cctgcctcga tccaagcacc aatcacaact aagggaatgt
     4981 ttggaaaagg gaggatgggg gaaagggagg aagaagacga cctatctgtc gtgcagttca
     5041 atctgctaat tttgtccaat gtcccaattc caaactctcg aagattttaa gtcaagcctt
     5101 agcctggccg agccaccgaa ggagatgagt cttcacctca aggccttgtg gtatgatgcc
     5161 aatgaggatt gggattcagc ccataaagct atccaggatg agggggatgc cttttcagcc
     5221 cggatccatg cctatctcca ccgaaaggaa ggcgacctgg ggaatgcaga ttactggtac
     5281 cggagaagcg gcagctccag gcccaggctc agcctggagg aggagtggat ggagttggtc
     5341 cgtcatggag ttcagatcga tacagggcat tagcgcccag gtatcgatca cccgatgtcc
     5401 aaagagagtg tagaggcctg tttgtctcgc acggagcaaa ggagcacacg gagattatat
     5461 aacaaagaag ggaaaggcga gtgagctttg gggttttatt tattgaccaa ccctgatcaa
     5521 aactccgtgt tctccatccc tccgcgcgac aacgatatgg aagtcgttcc ccatcggaga
     5581 tagtagcgga gtgaaggggt gattgcgagt caccccttca ctcccacccc agattccccc
     5641 catccggggg aaatgcgatc tttccaaacc gggtttattt gcctctttgc ttccaaactc
     5701 cggttcatcc aatgacaaag tccctgaaaa atcgaatacg cccatacgcc gccactctcc
     5761 aatcaatatc aaataaaggt tttaacctcc ttctgatctc catttttgga ctcaatatgg
     5821 cttttgccca ggaatccatt caaattaaaa tgcaggtcat ggatgagaat cagcgtcccc
     5881 tggaaggagc caatgtcctg atttaccggc tgccggattc cgtttttgtc aagggggaca
     5941 ttaccaatga aacgggggaa gcgaaggaac tcacgggttt taagagtggt ccgtattttt
     6001 ccaaagtgag catgctgggg tatagtcctt cggtcagcgg gtcttttacc cttcaggccg
     6061 gatccgccgt ccacgattta ggaattatac aattaaaaga tcaggccgtc aacctttcca
     6121 cggtaacggt gaccgcacag aagcccttta ttgagcgcag gctggaccgg ctgattgtga
     6181 atgtagatgg cagcatcgtc ggggcgggca gttctgcctt tgatgtactc gaaagatctc
     6241 cgggcatcct tatcgatgcc aacgacaata tatccatgag gggccgcagc ggggtcatca
     6301 tctatgtgga cggcaaacca tcccccatgt ccggggccga cctggccaat ttcctgagag
     6361 gtttgccggc caatgccatc gaccgcatcg acctgatcac caatccttcc gcccggtacg
     6421 atgcggcagg gaatgcaggg atcatcgaca tcaaaatgaa aaaggaccag cggctgggca
     6481 ccaacgggac tttttcggca ggttatggcc agggagtgta tcccaaggcc aataccggac
     6541 tgagcctgaa tcaccgcaat aaaaaaatca atgtcttcgg caattacaat tacgcttacc
     6601 gcctcggact caaccacttg ttgctggacc gcaattttta cgacaatttt gaattcagcg
     6661 gaaaggacga aaaggacaac tataccaaga tccccggcca ttttcacgcc ctccgctggg
     6721 gagcggattt ttctcccaat gaaaaaacca tcctcggggt ggttttgaat gccaatttta
     6781 atgccttcaa tccttttaac cggaattcct cccgggtctt tgatcaaaac cggaatcccg
     6841 tttttaactt caatacgaat accgacaatc acaatcaaaa caacaacctg atcgccaacc
     6901 tcaattttaa aagaagcctg gacagtaccg gcagggaaat cacggcggat atcgactatg
     6961 gccggttctc caccctgggc acttcatcca ataccacccg ctatttccag ctcaacggtg
     7021 aagtgctgcg cccggattac accctcctgg gggaccagga cggcctgctg gacttcatca
     7081 ccgcgaaggc agactatacc cacccgatca ataagaccac caagtgggag gccggtttca
     7141 aaacttcctt tgtctcctcg gaccaggatg ccaaattttg ggaaaagaac ggccaggaag
     7201 tcaccgtgga tgaaaccaaa accaaccggt tttattataa ggaaggcaat aacgcgggct
     7261 atctgaactg gagcaaagaa tttaaaaaat tcagtttgct ggtcggtttg cggggagaac
     7321 acacgcaaat caatacccgg caggaaaagg gaaacatccg tttcgattcc tcttatttcc
     7381 aattgttccc cagcgccttc ctgaattacc gcctggacaa aaaccagacc ttcagccttt
     7441 cggtcagccg caggatcgac cgccccggat acaattctct gaatcccttt ttattcctga
     7501 tcgacgtgac cacctatgcc acgggcaatc ccgtcctgct gccccagttt acctggaatt
     7561 ttgaagccgg gtatacgaga aaaacggtca acctgaccct ggcttacagc aggaccctgc
     7621 aaaatcaaac cgtgatcctt tccaggttca aagacgtttt cccccatatt ccctcagaag
     7681 aaaatgtgac ggtccagatc ccggtcaacc tgatctcctc ggactattac ggcctcacaa
     7741 tgtccactcc cctggccgta aaatcctggt ggaacatgat caataattat tccctgttct
     7801 acaaccattt caacggcaat ttgtccggca cctcgctcaa caacgggaaa gccgtgtttt
     7861 ccgggagcac caacaacaat ttcactatga aaaaaggctg ggcggccgaa ctttcattta
     7921 atttcaatac cgggaatcag tatggatacc tcacctttga acctcagtgg gccctgggtg
     7981 ccggcatcca gaaaaaagtg ctcaaagaac aggggaccct gcgtttcaac gtaaccgata
     8041 tcttctggac caatctgccc aaggccacca tccggtttaa caattatatc gaaaactggc
     8101 atgcctaccg ggaaacccgg gtggctaacc tgaattttac ctaccgtttt ggaaacacta
     8161 aagtggccca ggccaggaga aggactacag gttccgaaga ggaaagacaa agggctggaa
     8221 actgagcgct tcctttcctc ccttcctccc tcccgcggcc gctggactcc ttttcgaaga
     8281 aaacgtccta tcttgttttt atgaaaataa aatttctttt gttcctgctg ttcattcagg
     8341 ctgtgccggg attctgtcaa taccaagcca aatccaaggc tgagcaatcc atcctggata
     8401 attttaatga acaggtccgc tgctggaacg ccggtgatat agaatgttat tgccagggct
     8461 acctcaaaag cggcgaaacc cggatcgtct ccagccgcgg catcaccagc ggttatgagg
     8521 ccattttagc aagttatcaa aaaaactggc caaaggagag gatgggacaa ctgcatttcg
     8581 atcagctaag aatggataaa ctttcttcca aagcttacct ggtccatggc cgttttaacc
     8641 tgacttatcc ggaggccaaa gccctttccg gttattttac ggtggtcatg aaaaaagtcg
     8701 ggggaaaatg gctgatgttc gcggatcatt catcataggg tgtggaattg tggaattaca
     8761 gaattggtaa atggttacac gggcttccat ccattaaatt caaaagaaaa taatcaaatc
     8821 gcgtaaccct ttcccggtca ggaagtatat aacaggcgac ctggtctctg caaccttgca
     8881 cagagtttaa ttgtctttca tttatttcca acatactcca ccgccatgcc tgtcaaatat
     8941 agatacctga ctgccttttg gggcctactg atttgttgcc cacttttgcc agaacggttg
     9001 atggcaaaca atggggcctt agccattgcc tatcccatcc atcaaatcaa aatcgatgga
     9061 gacctctccg actggtcctg gataaaaacc agttatccga tcgccatcca cctttcagat
     9121 atcaaaccgc gggacgcctc tgatttccag gcctggttca agatcgggta ccacctggag
     9181 tcccgatccc tttacatcgc ggtcgaagtc accgatgacg accacatccg ggatacggca
     9241 aaaaatgcat cctggaactc gcgggatgcg gtagaacttg accttgattt gctgcacctt
     9301 cccggacgtt cgggtgtgac ttcttatttg ttgtccgtag atcataaaaa ctacaacaag
     9361 tcaaggtggg acccggtgac cccagccggt tgggacaaga cagaaggtgc ggtcatccga
     9421 cagggcaata aatcctttta tgaatggcgc gtggtgctgg aaggaccttt atccactccg
     9481 aaatccattg gcctggatct acaggttttt gacaaagatg ccggcgggga ttttagttat
     9541 tcggcctggg gaccggaagg ttataaatac agaaatccca acagcctggg tgacctcatc
     9601 ctgttgaaag aaggcgaaga accccatcct ctttcgggca gggtaaaatg ggctggtgaa
     9661 aagaaaatta attttccggc caccatcggc ctgaactccg aaaaggagcc tggtgtgttc
     9721 ctggacctgg ccctggattc aaccggaagg tacagcgcct cggttccggc cgggtcttac
     9781 cgtatggccg tccggaatcc ttacctgtca tttgaaaata aatggtatgt tctttcagcc
     9841 agcccttcgc cttccattcg ggtctctccg ggcgcgaggg tagaaagtcc ggacctggaa
     9901 ttggtatata ctccggtgcc cggtttaatc ccggccaaag gcatccttca tgatttcaat
     9961 gacctgaggg ccaaagaact ggatgccttc attgaaacct accaaaaaca atatcagatc
    10021 cccggtgtat ccctggccct gatcaaagac gggaaagtga tttacagccg ggcttatggc
    10081 gtcaagaaca gccagacccg ggaaaaaacc gatgaaaata ccctttatga ggccgcttcc
    10141 gtcaccaagc ccgtctttgc ctatgcggtc atgcggctgg tggaaaaagg aaagatcgat
    10201 ctggacaaac ccctgtatga atatctgcct tatccggaca ttgcacacga tgcccgctat
    10261 aaaaagatca cagccaggca tgtgctttcc caccggaccg gtttccccaa ctggcggtat
    10321 atgaatgcgg acggaaaact ggatatcaaa tttgaaccgg gcagccgtta cggctactcc
    10381 ggggaaggat ttgaatacct gaaaaaggtg gttgaaaaaa tcacaggtaa aaaagtggaa
    10441 aaaattttac gggaagaagt cctggatccc ctgggtctat accataccta tttttccaaa
    10501 aacgataccc tggccaaact ggtagccaca gggcataacg acaatatccc ctcgaacgac
    10561 gggttgccgg aagaaccggg catggcttac agcatgcata ccgaagcaaa agcgtttacc
    10621 cggtttatgc tcgccctgct gcatcaacaa gggctaaaac cggaaacctt tcgtgaaatg
    10681 ctcagcctgc aatcggaata taatacccct gaaggacagg agaaaccaaa attcagggag
    10741 tatatgggaa tcagcctggc tataagggaa tccccctacg gcctggtttt tggccacgga
    10801 gggaacaacg gggacttccg ttgcggcttt gaactttata aggacctgaa aatgggatat
    10861 gtgattttta ccaatgccaa ttcggctttg cctttgattg aggcgatgcc aaaagtattg
    10921 gtggagggga aagaatagat attccaattt ttgaaaattt ggaatatcta aaacacccca
    10981 tttaccgtat gacccaggaa acgcagcacc gtctcccccc agatgatgat catgatccag
    11041 ctgatcacca tcatcgtaaa cccgaattta aaggaatcga tgacgctgta ttgattggtc
    11101 gtatacagga gggccgccgg cttgctgttg aaggggagca catagacgtg ctcgatcagg
    11161 agagctaccg gaaatgccag gctcatgacc gggtaattga atttttgggc aatgccgatg
    11221 gcaatcggta caaagatcag cgtcctcatg gttttggcct ggaacaatat gccgctgaac
    11281 agcatgataa aagtcaggga gaggtataaa acccaaaacg gcgtctccgc ggaaatgcca
    11341 aaatacctga acagggcatc caccaggctg gaaggcaggc ccgtggagtc gaggccggtc
    11401 cccaatgcat aggcgcctgc cgaaaacaac atgaggtgcc agggaatgtc cacatcattc
    11461 catttgacca cccctatccc cggcagcaga gccactacgg ctcccataaa ggccaccgcg
    11521 gtctgtgaaa tcccatgctg tttatcggtt gcccataagg ccaggaccac cagaaagatt
    11581 gcgatggatt tgtattcatt cctattcatt ttacccaggg attcatactc ctgcttcaac
    11641 cgggccatgc ctccctctat ctggggcctt tgttcttccg gcttcatggg gaaaatgatt
    11701 ttagtgccca gccaccagcc ggccaggatc aggatcatcg caagcggaaa ggctgccaca
    11761 aaccagtcct ggtatccaaa aatggatttc cccatggccc cggcgatcag ggaaccggcc
    11821 aggaggtttg cccccgaacc cgtgatgaat ccggtagccc ccatgttgat ttgaaaaagg
    11881 ttttgaagga cgatgttccg tccaaaattg tttctcttgc ccggggtagc cccgtaaatc
    11941 gccgcgatca ccataaaaat gggcagtagg atggtggcct tggccgtggt ggccgaaata
    12001 aaaacggaca atacaatatt gatcacaata aaactgaaaa agatccagaa cgcatttttc
    12061 ccaaaacgca gcacgaacca caaggcaaac cgtttggccg cttgggtggt caccagcatg
    12121 ctggcaagaa tgaaggagag gatgttgagc cacatcaccg ggtgacccag ttgggcatag
    12181 gccaggttct ccgaggtgac tcccgtaagg accatcgtga ggatcaccag gagggaggtc
    12241 aggtaattag gcacggcttc cgtgatccag aggataatgg aggccacaaa taccccgagc
    12301 atggcatagt tgatgcggga aaattgttct gccccgattt cgctgagccg ctgcgccccc
    12361 tctttttgca ggatctcagg cttgatgtcg ttgagaaagg aaatatcggc aaaaaaataa
    12421 atccagacaa aggccaggat ggcaaaaagg ccaccccact gctccatcca tttctcaaat
    12481 cctttccggg ggattttttt caatttctcc acccggtaat tgttcatatc cagggggtca
    12541 aaaaccggga aggggacggc ctggtcttga tttttatcct ccggttctat gaccggtggg
    12601 tggaccgtct gtttgtttgt gttcatggct taaggccttt gggacttggg ccagcggcgg
    12661 tatggggttt tcatcagcat ggcattgtaa tactccacct ttccggtcac ctcattcccg
    12721 atccagccgg gttttataaa agcatcattt tcattggcca gttcgagctc ggccaccacc
    12781 aggccttcgt tgtccccatg aaattcgtcc acttcccacc ggcgcgtgcc ggccggcact
    12841 tcatacctgg tcttgtcgat aaccccgggc tcgcagatct tcatcagttc ttgcgcttca
    12901 gccaggggaa tttccttttc ccattcgtag cgggaagcgc ctgaatcatt tccgattcct
    12961 ttaatggtta aaaatccgga ttcccccttt atcctcaccc tgaccgtccg ttccggtacg
    13021 gaggaaagat atccctgggt aatccggatt tttttcagtg caatggcctt ataagcatct
    13081 gaagtcacca aaaatttttt ttcaatttcc tgtgccatgg tattttattt agttggccag
    13141 cggttggtcg atcatgccaa tcaccctttt aatggcctgg agataattga tgttttccaa
    13201 tccttccagg ccaaggtcct tcagggtttt atggacatcg gctgtttctg tggattccga
    13261 actgatggtt tccaccctgg ccccattgat caaaacctgg ccggtttcac aaatggtatt
    13321 attgacgaga taagcaaacc gccgtttgtg tacacgcacg gcctgcaaat cctggtgccg
    13381 cctgaccagt tccataaagt catcaaaaga atacgcttgc ttggtaagtt caggcaaggc
    13441 ggccccgaaa gcgggaaata cttccgtcag caggatttgg gcggtaaggg ggaactgccc
    13501 tttcatgacc ggattccatt gttccagccc atccagggtc tgtaccagcg ttttgatgtc
    13561 catcatgccg tcccggattt tggtattgtt aatatcgtta ttgcgggaga cgatatagat
    13621 ttcatcactt tgtctttccc agatattttc cgggacgggc atggacaagc gggccattct
    13681 tttggcgggg atttcaaatc cctgtccgaa agacctgaat tcaaacctgg gctttattcc
    13741 cagtccctcc gatggaaccg tagtttgcat caatggttgt tggaagtacc ccgggtcggg
    13801 gtggtgaata caacgaggtt atccgatcca tccggttggc gtccatagga ttttttaccg
    13861 gaaagactga tcgtctggaa atccaggctg gctacgagta tccctgcggc attttccaga
    13921 tagaccgcat ccccggaggt gcttaaaccg aactcgatgg catattcccc gcccgtatat
    13981 tcggttttca gcacaaagaa cgctttggat gggatgatgg tccctgcatt caggacccgt
    14041 ttgggtttcc cgccggtttt aataccctga tcgtaaaagg aataaccgct gatgtctaca
    14101 gggatatccg atccattgta tatttcaacc cagtccggat cggtttcgtc cttggtaccg
    14161 tctgcaaaga cttcgttgat ataaagctta ataaggttgg atgccccgac ggtatagttg
    14221 gcaaactggt tttcaacttc cggataccgg gaggtaaatc ccccgctgtt gaggacttcg
    14281 atataatact ccaccttggt accgaacggg gttgccggaa tggttccctg gtattcatat
    14341 tgcgcggttg aagtcatggg tatcgatgaa aaggtgccgc cggcaatgac gcggtacaac
    14401 aaatttaccg aggcaatgcc ctttaaatcg gtgatccttg ccgtgaccag cacgtcttcc
    14461 gaggaattgg gagcaaccgg cacctgtttc acattggaaa gggttgccgg accattgaac
    14521 aattcatctt ttacacagga actggcaaag agaagaagtg caaatacgct ccatatcaaa
    14581 tatttccgtt tcataggaat gagttttaga ttgtggttaa aagttaattt caaaacggac
    14641 gcccagcatg ccgaaatcat cgccagcttt gcccttcggg tctgcgacct gtctgggatc
    14701 tttggtaagg gctcccgtca ggtcatgacc cacaaatagt ttacccttgc cgctggcata
    14761 ccggtcgtga ttgacgtagc tgtaattcat catgagtcgt atgttctttg ccgcataaaa
    14821 attgatccct gccgtatagg cttcggcggc tccgccgtag atatccttgt cgttcatatc
    14881 gatattatcg tagcggaagg tcaattcgac atccccccat ttctgacccc tgtagggttg
    14941 gccaaactcc ccttcgagct tgttgtaata ttgccgccct ccgaaaagca gccagccagc
    15001 ctgtgcatag aaccctccaa aatcgagttt ggcgagatcg ctttcacgat gtacgcggct
    15061 ggtcaggtat tccgtctgga agaccaaacc ccggtgaaaa gcggcaaact caaaattgtt
    15121 tatgctgctg tatttcacat ccgggatgat atcggtatcc aggtattttt tccggttgac
    15181 attgctcagg gaacgggtgc tgtatctcaa tcctccccat tcggctacct ccacactggt
    15241 tttaggctgc ctgtaggagt gggccaccgc aaggtgaact ccgtaatccg ctgcatcccc
    15301 aaagggctgc aagaccaatt tttccgtgag ggagatccct tcatccctgc catagtcttt
    15361 attgttgtct tccacgaagg ttctcagttc ggcgtcatcc acctcctgga aaaagacgcc
    15421 ggcggcggtc ataaaatatt ttccgctgta ggagccctca agtccaatat gcctggaagg
    15481 ggtaaaagct tccacggcca tgctcctttc ctggaaattg acatttcgtg aagaggtgtt
    15541 ctgcagcatg gaaaaccgtt ctttgaaatt tcctgcccgg acggccagtc cgttgcgaaa
    15601 atcgtacatg agataggcgt ctttcaattc cagtaccgcg ttggagaaat caaggtcaag
    15661 ctcaccgtac cagttccggg taaggttggt tttgactgcg aaccttgccc gccggatgga
    15721 ggtgccgttt ccaatggcgt ttaaggtctt atccggaaaa tagtgtccat ccacctgtac
    15781 ccggatgtcg aaccagagcc ggtaatcctg gtccgccgat tcgaaaacca gaatgccgtt
    15841 ccggttttcc acatccaggg gcgatctgct caccatctgg ccgtactggt taaaccggtc
    15901 ctgcctgctg gtgtcctggg caaaagcaat ttgtatcagg aaggctaatg ccgcagtaat
    15961 tgctgttgtt ttcataatta ataatttatg gttaaaccgt attgatgtat tgaattaaat
    16021 tgtcttcttt cctgaggtct aattttttcc tcaaccgata cctggcgatg tccacacttt
    16081 tgtgagaaat cccaagaagg ggggcgatgt ctttgctgga aaggccaagc cggagcaaca
    16141 gggccagttt tttttcctgt accgtcagat gagaaccggc tgccgttagt ttcgaaggga
    16201 gcggcccgat cgccgtttcc aattccatgt aaaaccggcg gatttctgcc gagtcattca
    16261 ttttttgatg aagtgcggtg agcatcccct ggattcgttc attctttttt tgcagttccg
    16321 gctcccgctg ggcttgttcc agggaatccc tcatggattc cagaaaacgg cgttcgtcgg
    16381 aaagatggag agcatgtttg accactgcat tccgattggt atcggatagc ccgggttccg
    16441 gtttgattat cgcttttcca ggtataccct ctgctcccaa aaactccccg gacaaagggc
    16501 gatatccatt atttccgtcc cattgcccca atggataccg ggaccggacc ccttgtccgg
    16561 ccgttctgaa ataccatccc cccgcaaaca gagccacaac caggagggcc aaaaagagcc
    16621 ataaaaccat gggataagcc gggaaatgga agtggaccgg aagatcttgc ggcgcattca
    16681 cagcctgttg ccggctcatc acagggattt caaatacatt gctgataaca tacagactga
    16741 taaaagtcag gccgcctgcg aagaacgggg tcagcaacca acccccgaaa atgctgccca
    16801 gcagtttgaa gcggatttcc tgcactccct tgaccatgcc aatgcctatg agggatccca
    16861 caatgacctg ggtgctcgat accggaacca gggggattgg cggaaggccc atgcgcacca
    16921 cgaaggcaga cagtgcagac gaagaaaaca ggaaaagcac cagggcctgg gacaggacga
    16981 cgaccagtgc ggtctccgga gtgagttcga ggactccttt tcctaaggta agcatcactc
    17041 ttttgctgta agtgagtatc cctgcggcaa tcgccaaacc ccctgcaaaa aacaggacct
    17101 gctcggaagg aatgatcaga ttcccgattt gaaccgtata attggaagca tttacaaaca
    17161 cccccatcac attggcaata ttgtttgccc caagactgta agcgccaaat gcaccggcca
    17221 ccaacaggcc cacgcggata cggccgtcaa gtttgatcac atggactctt gatttccgga
    17281 tatacgtccg catcagcaag tacaggagag cggcgaatac cgctcccaaa acaggtccgg
    17341 tcaaccagga agtcatgatc acgaccagag ttgtataatc caccgggctt gtggtaaaaa
    17401 aacaccagcc gatgatggcg cccacaatgg cctggcttgt ggagaccggc agatttttat
    17461 atgtcatcca cgctactacc gttgccgcgc aaagtgcaac ggtaaaagcg ccgcccaagg
    17521 cattcaccga ccccaattgg gaaagggtcc tcgtggcccc ggctccttgc agagtggccc
    17581 ccaaaatgac aaaaagaccg gcaacgattg ccgctttcct gaacttgatc atgccggtgc
    17641 ccacggctgt tccgaataca ttccccgcgt cgttggcccc gagggaccac cctaaaaaca
    17701 aaccgcttgc caggaataag aagatcatag tcagacaatc cttttaatgg ccataatggt
    17761 taaaagatca gctacctttt ccgccgtatc cgaaatagtt tctatgtgca gggtgaaata
    17821 tctcaggtgg aacttttcac tgagccccag ggccggcatc tccttaaaaa tctttctcct
    17881 gaccacatcc gcgaaaacat ctgtttcttt ctcatagaaa taaacccggt ggattttttc
    17941 acttactgcg gccggctggt tgaaatagga tttggccgcc gggatgacgg cttccgcagc
    18001 cgacaacgaa agctgggtga gtttaataaa gtccaaaacc agttcctccg ggatcagggg
    18061 attttccaca tcaaactgga acaagttctt tttagagagg tcgatcaggt tatccagttc
    18121 ttccagcagg cgccagatat cgccccttaa ctggggcatg agggactggg tataaagggt
    18181 ttcttcgatt tttcgccgga tcaggtcgga ttccgcttcc agtttcgcga gctcctgcag
    18241 attattggca aaactcgcgg tattctggtg gagataattt ttgacgcctt ctttaaagat
    18301 cagcagactt tgatccaccc cgttaaagaa tccatcaatt aattcaatcg atttattcga
    18361 tctcgaagaa aacatttttc ctccttttct atcaaaatta ccctcagggc cagtcctttg
    18421 tcctgataga catcagcact taatatgact gaaggaatgg tagggccttg gtatggttat
    18481 acccgcttga aaatcaaaag cgatcggagt ggactgtata ggacgatgtt caccagggat
    18541 cccggcgccc gtagtgaatg cccggctgca ttatttattc ccttttggca ataccggtca
    18601 atattgcctc aagacctgtc ttgttttttt ccaatacctg ttttacatcg gcttcttcca
    18661 catgacccag gatacccagt ggcccggtat agcctttggc cagcagagtc cggatcatgg
    18721 cctgttcttc atcgccttcg ttgagaggta agatcttggg tccatttcgg cgcatgccgt
    18781 tcaggttgac cgctttgagg taggggagca tggtatccaa aagcgagtta tagcggtcaa
    18841 gctgatggtg gacatggtga aaactgtata cgatgccgat atcttgcccg ggcaaggccc
    18901 ggatgatctg tacctggttt tcgggttccc caaaccagtc cccgtgattg taaaggacca
    18961 gtttacagcc cagggacttt gcacgactgg caatatagtc cacgatggcg atgcctttgg
    19021 ccagggcctg gtcctgggta aggctgtcaa aataattcgc attaaagctc agccagaggt
    19081 ccgtatgcag gccggcggaa tccaggttgg cgaaaagcct ttcattggcc ggactgagtt
    19141 ttccaatcgt gtccgtcctt tcatcgagcc aaagccagac tgcgctgact tcaatcaggt
    19201 tttcccgggc cagttgccat tcgtgcacca tttcttccaa atgcctatcc ctccagtcgt
    19261 aagcgtattt tttaataccg agttccttga gcatttctat gcgctgctgc ggggtccgct
    19321 gcatggagtc aaaaggcacg atgcaccagg caaagacacg gtccatatcg attgcatctt
    19381 cattcctgtt gtttttacat tgaatgaaca acaaacaggc cggcaggatt gtcatccaga
    19441 atcttgaagc cttcatgggt gtattttaaa agaatcgatt gggtttacat ggcataacaa
    19501 cggtccatcc ggtctgatat ttctttcagc ctcagccggt ctccgccatt cacctcggcc
    19561 gaaccccaac cctggtaacc tacttcccgg agtgctttat tcaccgccgc ccagttggca
    19621 tcgccctccc cgagttcggc gtcaaagcct ttccatatcc cttcatcgga agctttttta
    19681 cggctgtaat ctttgatatc caccttgagg atccgtttgc ccagggtgcg gatccagtgt
    19741 tccggccagc cgtacctcac aatattgccc acatcgaaat accatccgat catgggatga
    19801 ttgaattcat cgatgtaccg cgtggcttcc agggggctca gcagaaaatt gttccagaca
    19861 ttttcgaggg cgattttaat gcccgtttct tctgcaaaag gcaggatttt ccggatctcc
    19921 gcctgggacc tttcataggc ttcggcatag gatacctttt cattgaccac ggcaggaacc
    19981 aacagcaccg tggtgccccc gtatttttta caatccctca atgacgtttt catcgattcc
    20041 acgcatttgc cgcgcacggc cggatcggga tcggacaggg gcgaattcca gtgcagggag
    20101 ttgaccacgc cgggaatctc aaggcccgtc ttatcccggg catccaggat ttctttttcc
    20161 tgcagcgggt tcggactgtc caactccacc ccgtcaaaac ccagatcctt aatcagcttg
    20221 aatttttcca gtacggtggc gccttccttg atcatgccgt atttgaggct ttttttagta
    20281 aggggctggc ctggtccgta aggaatattt aaaggcaaaa ccatggaggc tgaggccagg
    20341 gtgctgtact tcacaaactc cctgcgtttt aattttgaca taaaattctg tttatgttcc
    20401 gggatcgaaa cggtcgttga ataaaataga acaaaaaggc tgtcagggcc tattatgtaa
    20461 acggggttac tccgggacgg gccacctcct tgatcttcca gtccaggtcc cagttgtatt
    20521 catcgatgtg gggagtcagc atttcttgcg aattcagcgc ttcgtcccag gtgaggtctt
    20581 tgccggtata agcgaccatg cggccccaga tggccagcat ggtgctgtgg gcgctgcgga
    20641 tgccgtcatt gatggggtcc cctttccgga tggatgccat caattcgtcg tgctcggtct
    20701 ggtacatatt gtttttatcc ttttccgtat gggtcttctc ccaactccag ctttgtttcc
    20761 ctttgatctc gtgacgattc cagacattga tgtcgcaacg gcctttggtg cccagcaatt
    20821 caataccgta agcgccactt gtccctttct gttgcctgct gtgatggaag cccctgctgc
    20881 catcgggata ttcataggta atggcaaaat gatcgaaaat attgccaaac tcgggttcga
    20941 tccggctctg cctacccccg gttccggatg ctttgatagg aagtacgtcc cctttggccc
    21001 aggacatgag gtccaggctg tggacggcct gttccacgat atgatccccg gacatccagg
    21061 tataatacac ccagttgcgc agttcttttt tgaattttcc ccagctcggt ttgatctccc
    21121 gccagcctcc ggcaccggta ttgtacgtat tatagatgga gaggacatcc ccgatttcgc
    21181 catccaatac ccgctgaaag gtttcgcgtt taggaaagtc gtaacgccag caaaaacccg
    21241 acatgaagcc cagttttttc tccttggctt ttttagccac ttcgatgact ttccggatac
    21301 cggctgcgtc cacggctacg ggtttttcaa aaaatacatg tttgcctgca ttgatcgccg
    21361 cctccaggtg ggcaggtctg aaattcgggg gagtgcctga aatgatgatg tctacgtcgg
    21421 attcgattac ttttttataa gcgtcgaagc ccaggaattt ctgattggcc ggcaccttga
    21481 ttttatccgg agcttcttcc atcagcgaat tgtaggcgat ctccaatttg tcttcaaaga
    21541 catccgccat cgcggtcaat acaatgtttt tatccccccg gacggcctgc aaagcggcgc
    21601 ccgtgccacg gccaccgcag ccaatgatac cgaccttgag ggtttcggcg ctgaactgat
    21661 gcttgggata aggattggct attacattaa agcccatggt gcttgctgcc agggttgaac
    21721 cggaaagcct gataaagttc ctcctggatg cattggttag ttttttattc ataacagatt
    21781 ttatattcgt gaataaacaa aaagggactg atcaacaaag tgttcgtaaa attacgggta
    21841 taaatgattg aatcatatcc taatattcat tttcttggat caaaaaactg gggcaaccag
    21901 gttttgaaaa atggaaggcc ttccccgctg ttgccggccg aagcataatc gcaaagatgg
    21961 acggctatgg gttcagggcc tccttccgcg taggattcgg gtagaaaggc cgctttgaat
    22021 tgcatccaga tctcctgatt tgatggactt actttctcca actgaatata accggtggaa
    22081 tcccccaccg gggtcagaat ggattccagg gatgggccag ggagccggcc atcccttgcc
    22141 aggaccaggg ggccccaggt aatcgcctcg tgccgatggc ctgcaccgga attatggatc
    22201 cgacccctca aatcggtttc cagataaacg agatccccgt ttcgccacct gcgtccgatt
    22261 tcaaaaagac ttccggcctt tccctccata cggtcctcct cattgacctt aaatttcatc
    22321 ctgctggtcc aaccgggtat cctgattttc aaagaaaatt cttccggttg ttccatttcc
    22381 agaatgagct ttataattcc cgaggccgga tagtcggttt cctgcaggac tttgatttgt
    22441 tttccattcg gagtcttcag acggtatgaa cctgctgtaa aaaagttgac ctgaatgccg
    22501 gactcatctt ccatcaccgc ggtgagcgga agggtgtaca aggcccttgg gccactggca
    22561 ttgcagcagt tgaggcccat gccgcattgt tcgctccctt ccagtcgctg accggaaaga
    22621 ggggtgtatt tggcccagtc cgacccatct ggtttcaggg cgcccagcaa tgcattgtaa
    22681 taactttgtt caatggcatc tgcatatttt gcctgcccgg tcaggcgcaa caattgctgg
    22741 ctcagcctga tccaggtggc ggtcacacag gtttcctggt aatggtacac cggttcggac
    22801 tggtgttcac gtccatgaaa ccaacattcc acggaagaac cggagcccgc gatattgatc
    22861 tccgtctcct ttatattatc ccaggtcgac tcgacggcct ggaggtattt tgcttctccg
    22921 gtcaggcggt acaattccag gagcccttca taacaggaca tcatttcgta ggctttttgg
    22981 ccttgttccc agccatacca gtttttttgc ggttttggaa accgggaagc cacgtccact
    23041 ccggccttgg atatcagttg cgggccatcc ggactttccc actggtttac gatttccctg
    23101 gcaaaatcaa gatattgttt gtttcccgta cgggcataaa gcaggcagat gggctcgagg
    23161 atggaggtgg cggccatgcc gcgatgattg cccaggcgga ctatgctgac ctgcttttgt
    23221 ttgagttcca tcatgaggtg attggccagc cggcctgtcg catccagtgc ttttttatct
    23281 gcggtcagat cgtaatagga aaggagtccg agcatacaat atttccgacc ccagatatcc
    23341 cagctctgaa ggtggcggtc cggtgcatag ttgccgatat aaccatccgg gctttgagtc
    23401 gcaagcaggc tttcaacggc tttatccagg actgttttaa gtcgtgcttc aggccgatac
    23461 cggtaggcca gcaccgcgga ggtaaaccat tttccccaga attccgattg ccagcaactg
    23521 gtttcggtgc gttttctgaa gggagcgacc aggcgatcgg ggtcctgggc caggatcctg
    23581 ttttcataag atgaatcgag cctgcggccg aggtaaccat gtatttgggc tgaacttgca
    23641 gccttcaatt gatcctgcac tactggcttc accggcgtct gtgcggggct tcccgggatc
    23701 agaaaagcca ggacgaccat taatttccat atacccatca tggcttgaat ttaaatgatt
    23761 tagtgataaa gtaccataac ctatctttat ttcccaatgc cctaaaatcc ggctcgatga
    23821 aaaaatgtat tctccttctg ctctctactt tagccccggc tctcaaagcc cagttttcga
    23881 tccagaatgt actgtctccc ccctttccaa cagaacttaa ggcttcaggt aatggccata
    23941 ccctggcctg ggtatttaat gaccggggtg aacggaatat ttttgtttct gaatcccctt
    24001 ttgatcaaag ccggcccgtg acgtcttaca ccggggacga aggattggaa ctgggtcaat
    24061 tgaacataag taccgacggg caaaggattg cttttgtccg gggtaatgct gccaacagtc
    24121 ggggcgaggc cgccaatccc gccttccttc aagtaccaac cgggcaaagt atttggatca
    24181 tggaccggga tgggagccat ctccggaata tagggccggg caccagccca tccatatccc
    24241 cggatggcag gcaattggtt tttctccacc gcggggaagt ctggatttct gatttaaacg
    24301 atacggcaaa accggttcaa aaactatttc aatcccgcgg taccatggct caactcagat
    24361 ggtccccgga tggaaataaa ctggctttcg tgagccaccg gggcgatcat tcttttgtag
    24421 ggatatatga tttcagtatc cggtcggtgt actttgtaaa ggccggcgtg gatcgtgatc
    24481 agtatccggc ctggtccccg gacggagggc agttggcata tatccgggtc cctcatatca
    24541 ataaaattcc tccctttacc gcggtcagga cttccaatcc ctggagcatc cgggtattcg
    24601 atttgggctc cggaacagat agtgaggtat ggaaggccga tgaagggagg ggcagtgcct
    24661 ttaccaatga aattcccgtc gttgaaaatc cgctctggtg gctcgatgaa aaccagatgc
    24721 tctttcctta tgaaaaaacc ggatggcaac tcctgtatac tctggatatc ggcaaacaat
    24781 ccgtttccct gctcaccccg ggcaaagggg agattgaaaa tgtttgcctt agccctgacc
    24841 ggaagaccct gtattatacg accaatatcg acgatcccga aaggcgccat atctggaaga
    24901 tggatttgcc cgatggtaaa ccgttttgta tcactccggg catgggcata gaatggtcac
    24961 ccgtacccat atcatccggt ttagccttct tgcaatcttc tgcaaaacgg ccggcctggc
    25021 ctgcggtgct taaaggcccg gcaagacagg acctggctgc cgaaaggttt ccagcggatt
    25081 tcccttccgc cctggttgag cccaaattgg taaaaatcaa atctaccgat gggatgatgg
    25141 tcagcggaga tctctttttg cccccgggtt atcaccccgg attgaagtat cccgccctga
    25201 ttttcctcca tggcgggtcg aggaggcaga tgctcgtggg atttcattac agccagtatt
    25261 attccaatgc ctatgctctc aaccagtatt ttgcttcgcg gggttatatc gtactttccc
    25321 tgaattaccg gagcgggatc ggatatggcc ttgattttcg agaggccctc aactatggcg
    25381 ccggaggggc gaccgaagtc cgcgacctga tcgctgcggg ccatttcctc cgaaaccgga
    25441 ccgatgtcat catgaataag atatccctct ggggcggcag ttatgggggt tacctcacgg
    25501 cacatggcct ttcacaggct tcccagcatt ttgcctgcgg ggtggatatc catggcgtgc
    25561 acaactggaa tgacgagatc ccaacttttg ccccctggta cgatgccgcc aaatttcccg
    25621 ccatcgcgaa gatcgctctt gaatcttcac ctgtgcacta cgtcaaaagc tggatccggc
    25681 ctgtgctgtt tattcacggc gacgatgacc gcaatgtacc tttcagtgaa acggtcaata
    25741 tgatccaaca gctccgtaca cagggcgttc ataccgaatc cctggtcctg cctgatgaag
    25801 tgcatggttt tttgctgcat aaaagctggc tgaaggttta tgaagccagc tttgaattta
    25861 tagacaggca gatgaccgga aaataaactt attccgcatt tgacgtttca aatgttgaaa
    25921 tttgatgaaa tgctccagaa atcatgatga aatatttcat tgccctgctt atccccctgg
    25981 cccttcattc ccagaaacat tattcagccc gcttggatca actgatgcag gcaaaatacg
    26041 aagtggaggg cttcagcggc accgtcctgg tgatgcataa aggggaaaaa gtctatcaca
    26101 aagccttcgg actggccgac cgggaatgga atgtgcccaa taccccggat accaaatacc
    26161 gcattggttc ggtgaccaaa caatttactg cagcctgcat cctcaaactg gaagaggaag
    26221 gcaaactcaa ggtgagcgat ccactgagtc aatatttccc ggattatcct tatggggaca
    26281 gcatcaccat tcacatgctg ctgaaccata gctcggggat taaaaattat accagccttc
    26341 ctgaattctg gccaaaagcc gtcctgccgc tatcacatga ttctatggtg gcaatattta
    26401 aaaacaaacc gctggatttc tcccccggca gccggtgggc atacagcaat tcagggtatt
    26461 acctcttggg cctgattgtt gaaaaagcat ccggtcaaac attccaggac tttctccggg
    26521 attggatcat tgagccggcg ggactaaaaa ataccgggat ggaccgcctc gactccatat
    26581 tgaccaagcg ggcaaaaggg tatgggtgga agtcccccgg tggatggcgg catgccgatt
    26641 atatttctat ggaagggccc ttcagtgcgg gggccatgtt ttcgaccgtt gaggatctgt
    26701 acacctggaa aaaagccctg atgtcaaata aaatacttta cccgggttcc gtggccaaaa
    26761 tgactacccc ctacctggga gcttatggtt acggattgtc catcgattca ctgcataccc
    26821 gtccacgcat ctggcacaat ggaggaatac ccggtttttc cgcccacctg gcttattatc
    26881 cccaggacga tttgtgcgtg gtggtcattt caaacaatca gtccagcgcc gaccgggccg
    26941 ggatcgggct ggcctccatt ctttttgaca aaacggttta tattccttat ctgcccaaag
    27001 aaacctacct cgatccgaat ctgctggatc gatacaccgg aaaattccag gccagcagcc
    27061 cgcttgaaat ccggagggaa ggcaatgccc tgttccggca cacggaaaac ggcccaccca
    27121 ttgaattaaa ggcggaatcg ctgacccgtt tattttacac ggaccgctcc gggcggttct
    27181 tggaatttga atttgatccc cagggtcgcg taacccgggc ctggtttata cagaatgggg
    27241 aaaaaacgga aatgaaaaaa gtggattgac cctgaccgat tcccatgcgg tacttaagtt
    27301 ccaattttat attccatgcc ttctccggcc atctcaaaag gaaaatcata ttggcccgtg
    27361 agcaataatt cggtgagcag gtcctgaatc tgcacgtcgg tcctgcccgg gtcgtgatga
    27421 gcaagcaaaa gcttttttac tcccgaaagg gtggcaaatt tcatggtatc gcttaggccg
    27481 gaatggcccc agccttgttt ataccggtat tcttcatccg tatactgggc atcgtggtat
    27541 aacagatccg ctccagccgc aaggtcgtag cccgatatcc aatgcggatc atcaaggata
    27601 ccggccaacc caagggccgg ttcgtggtcc gggatatagg caaatgcgcc atttccattg
    27661 gagatccgga aacccacggt gggccccgga tggatcacat atcgcgaccg gatccggaag
    27721 ggcccgattt caaattcgct gttggcaatt tcatgcagat gcagttggca gggaagatcc
    27781 cggatcagga ccggaaacaa agggggcgaa agataccggc tcaaccgggc atgcaggcta
    27841 tgggtgctgc tggcgggccc ccagatgtgg acctccatgg agggattaaa aagggactta
    27901 aaaaaaccaa gcccctgcag atgatccagg tggaaatggg tcagcaggat gtctaccctt
    27961 ttaccggtca gttcctgccc gaaattcagt ttttgcattc ctgaaccacc atccaggaca
    28021 aggaaatggc cgtcttggga aacgacgacg caggaagtat gcccgccata ttgccgggta
    28081 tccggatccg atacgggtat ggaaccgcga acgccccaaa gtttgatgtt catagtctct
    28141 cgtctttcca gaagatggct atgccccccg aaaagttgcc cgacctgcca ataatgggaa
    28201 aagaagtgac cgagattttc tgggtttttc cctgcaggct ttctatccag aaggttttgt
    28261 gataaggata aacattccgg agcgtcttga ccaggggcaa atcctcagga gaaagagggt
    28321 ttttaaattc atccacgggt ttgaagattt taccccactc ctccaccggc atcaccccgg
    28381 attcttcaaa ccgtttgccg aggatttctt cggccggttc attgtaatag atcaggttgc
    28441 cttccgtatc cgtaatgaat accggcatcg aaaggctgtc tgccagctga cggcttagaa
    28501 tgatctcgat ttcgtaggat ggcatttcgg ggttgcttac ttcagttaaa tataggcaga
    28561 tttctcaaat attatcccgg aggacggagg cccctttccc gcagttactt atctttcgcc
    28621 aaaaaagatg cgtacccttt tatttttaat tataaccttg tttgtcatgc cggatgtcgc
    28681 tttttcgcaa actgcctctg caggcaaagt actccgtcat gtcgtcctgt tcaaatttaa
    28741 agagaccagc agcgccgcgg acattcaaaa ggtggaaacg gctttccgga acctccctgc
    28801 aaaaatcaag gagatcaaag attttgaatg gggcaccaac aacagccccg aaaatctgaa
    28861 ccagggtttt acccactgct ttcaattgag ttttgccagt gaagccgacc gcgcagtcta
    28921 tctcccccat cccgatcaca aggcttttgg caaggtcctc gggccgcacc tggataaggt
    28981 gctggtggtg gattattggg cggagaagtg agatgccaga tacgagaagc gagaagcgag
    29041 atacgagaag cgagaagcga gatacgagaa acgagatacg agaagcgagg ggcgaggtac
    29101 tcagccttag tctcaacctc aaccttagtc tcaacctcaa ccttagtctc agcctcaacc
    29161 tcaacctcag cctcatcctc atcctcaacc cacaaaatga agaaatacaa tattattcta
    29221 ctgaccttgt ttttatcccg gcatgccttt gcccagccgg ccgttaagga tgaaccaagg
    29281 catcacctgg ttttcgaaaa tgaatatctg cggatcctgg acgtccatat ggctccgaac
    29341 gacaccactc aatttcacct gcaccaaacc ccttcggttt tcatttctct cagcaagacc
    29401 ttaaccacca atcaaagcct gggccagccg gtcaacccgg tccgttacga cgagatcaaa
    29461 gcctggtacg atcccatccc cagtccacgg atccaccggg tttggaattc agacagctcc
    29521 tggttgcata ttatggacgt ggaactcact gcggtccggc aaaagtccaa tccccctgta
    29581 atccagagtc ctttcctcca attgcttttt aacgaactgc aggtgaccgg gtaccggctt
    29641 gaacttccgg cggataattc catagcattg ccgatatccg atacggggta tctcctggtc
    29701 agcctcgcaa attcggttat ccgttttgac atgcatggcg ggtcacagaa acgaatgatg
    29761 aaagccgggc attatatatg gatcccggcg ggaggggcct ccacattaac caacctggat
    29821 caaagcaagg cggaactggt attgctgcat ttgaaataga aagcgattta ggaaaatcca
    29881 tttggtaaaa taataccgat gcgcggaggg tgaaaaatca aacggaagct tttatccgct
    29941 caagcctgtt cgaccgggat caacaattta taccctttcc catgcacccc tatcagtttc
    30001 acctcaggat ctacggacaa tttttttctc aggcgggaaa caaagacgtc caggctgcgg
    30061 ccgactatga tgccctgatc ctcccagatg gttttcatca actggtctct ggggatcagt
    30121 tgattgggag cctgggcaaa ctgggccagc aattccgatt ctttcgcgga tagcggaatc
    30181 ccgtcctctt caaaaaacaa ggtctgatcc gatggattaa aacggaaacg gcccagaaaa
    30241 acgactgggg aagaccgggt atgctgctct tcagcgggtt tgtgggcggc aggaccggat
    30301 ggcttgtttg tcctcttttg caaaaaataa cctgcgctga taaaaagggg gaacaggagg
    30361 aaaccatacc atttaaaggc tgccggaggc tctttaaaaa ccactctcat ttggtaacaa
    30421 gcttcaggag cggcccggcc ttcacaggga atgaccggct gatggtctct gggatcgaca
    30481 gaaaagctgt aaacgatgtc gctggtcccg caatccagga cctggaataa ataagccctc
    30541 gaaaaggaaa cgccttccag atgttggcgt acgacccgga ctatggaatc gggtacgaat
    30601 gaaaataccc gttcaaaacc aaggatgtat tccgttccct tcagccgccg gagcggcaat
    30661 atccttgaac tgtcatcccc tatgaccttc aatatttcat gtcccatcag ccggagcctc
    30721 acttcatcct gatccgggtc gacgtccgca agcgcatacc ggttgcctgc ccaggaaagc
    30781 aggaggaagg cggccaggct aagcgccagg gcataataaa tccaccgggg tttcatgttt
    30841 ttgcaaagct aaggccggga ggagcaggac gaggggattt ttactctttt ttacaatcat
    30901 tttacatccg ctttaccatg gggagtccgt ccaaccggaa tcttgtcccc attaaaaaat
    30961 cagaattatg ataaaatcgt ttacgaccgg gtcttttcct gccggactgg tcctggtaag
    31021 ggtgattaca ggatttttac tcatcagggt gggttgggaa attacttccg tggataaaat
    31081 gtccggttat tttcaatggc ttaccgacat ccggtttccc ctgccggtcc ccatggccta
    31141 tgccggaaaa attgccgaat tggctggggg cgtgagtttt attaccgggt ttctggtgcg
    31201 ctggaccagc ataccggtaa tgatcaccat ggcggtcatc accttcatca tgggggaagg
    31261 aaatctgatg agcgattcct ttttattgct gctgctggcc tgggtatttt tctgcaccgg
    31321 cgcgggaaaa tggagcctcg atggattcct gcctggcctc cggcccggca aaccaatcat
    31381 ggccaaaagc caaatcctgt tttttcctgc cttgtttttc tctttaaatg ccatcacggg
    31441 gcagccggtg gagccctgga tccacaaacc ctttgaccaa tggccggaga tcgccctggt
    31501 caaccggttt tctttgaagg acggaagcgg atacgacgat ccctccttgt cctatgccgg
    31561 cacggccttc ctggtgcgta aggacgatgc cgtttttgcc gtcaccgtca aacacatctt
    31621 gttcctcgcg tggagaaaat tcaataaccg cgtttttatt tcagataaaa tcggtcgatg
    31681 gaccatgcac ttaaaaaata atcccggact ccgtttggct gcggggccgc tcctgaatga
    31741 ggacccggac gaaaaaatac aggacggatt tgaaaacggg gttcttcaaa gggattggtt
    31801 gatttttaag acggattcca tccctgaaaa aattcatccg cttaccattg gttctcatct
    31861 tccttcctta ggcgacaagg tttacctcac cggcaatccc taccggatcg accatacggt
    31921 ccatttccgg ggaaaggtgg tcaaacagga aggtcctgtt tttctggttc aatttgaaat
    31981 gcccgatacg gtatttcttg gcggggccag cggatcccct gttttggatg aaaacgggca
    32041 cctggtgggt atcttttcga catccaggcg agatccggcg agcggtcgga ttcagtatat
    32101 catgaatacc acccattatc tccatgccgt tttggagaac aataaacctt tgaacctgaa
    32161 ccgaaggccc ctggccacat tggttgactc cctggtaagg aagcattcgg taaaaaccgc
    32221 catggatcag gttaaaaaaa tgtatcggga tcccgatgct tatttccgtt ataacctgag
    32281 ttggccggat tataaagctc tggatcaaac cggccgtaga ttgattcttt caggaaggac
    32341 ccgggaagcc aaccgctatt accggggact gcttgatcag tatcctggta tgcatttttt
    32401 tgtgttatcg atgctggata ttcttgtaca tgccggtaag aaggagaagg ccctgaggtt
    32461 tttagaacaa gccattcaaa aagccgatcc ggaaaccaga accatcctgg agaaaaaacg
    32521 aaatgaactt acctgttagt tcgaagctga gttccggttt tttgtactcc tcaaaatgca
    32581 ttacgttggg tcgaagcctg gcttcggcca gcctttaata tgaaaaccac ccggtcggag
    32641 tcgtctaagt gaagggttaa atcaaattca ccccttcact tagacgaaaa aggccatcct
    32701 tattcagaat ggcctcataa ccatgaaaag ctatacaatg atgtattatg cttttttctt
    32761 tttttcggct tttggcgcgg ccgcctttgg cgctttcgca accggagccg gtttgactac
    32821 agcagcagca acagggctgg ccgcggtctt taccttgtgc ggacgggttt taccgtagga
    32881 tccgattgtt atttttccgc gttttgatct cttgtcacct tttcccatat aaaattttga
    32941 ttatgaatcg gcaaagatag gaagattatt tcagtaatca gctttcagct ttactgacca
    33001 ttgactgtta actgttgact attgatttat cactattgac aaaatgaaca ctgttcatta
    33061 tatttgcatc attaatttaa ttttatgagt atagccgacc gaaaagaccg ggaaaaaaag
    33121 gaactcaccg aactgatcct gaaggaagcc agggccttgt ttctggagga aggttttgaa
    33181 aaaacatcga tccgccggat tgccgaccgg attgaataca gccccgccac gatctacctc
    33241 tattttaagg acaagaatga cctgttgctg gcccttcacc aggaatcgtt cacccatctc
    33301 tttgaagccc tttcgtcggt ggcttcggtg ccggacgcgt ttgaaagact ggtggctctg
    33361 ggaaaggcat atatcgagta tggcctgaaa aacccggaag aatacgaact gatgttcatg
    33421 ttgtccgccc ccctcgaagc cctggaatgc aggcatgaac tctggtgcga cggccaaaaa
    33481 gctattgacc ttgtaaaaat gatggtggat gattgcaaag cacacggcta tttcagaaaa
    33541 gacctggatc cggataccac cgcaatcatg ttgtgggcgc aggtacacgg actggtatcc
    33601 ctgtacctga aacagcgctt caaaatgttc caggaccagg accagaaaaa actcattgat
    33661 aaaaccttcg ggctttttgt aaaaaccctg cagtgtttcc gctaaaccac acccctcttt
    33721 ccccattaag ctcaattcaa ctctatgact tttcgaaatt cggcttggat ccttgccctg
    33781 atcttcctct atcccgggct ttcggcccag gcccagcaaa atgccctgga tgattacatc
    33841 cggcaggccc tggaaaaaca cggcctgatt aaagagcagg atgccctgat cgacgagaaa
    33901 aaagccggct tcatgctggc aaaccgccaa tccgggccgg aactcaccct gtccacttcg
    33961 tatacccttg ccgccggagg acgggatatt tcttttccac tgggcgacct gctcaatccc
    34021 gtgagcaatg ccctgaacga ccttacccgg acccaggact tcccccagta tgaaaaccaa
    34081 aatattttat tcttacctaa caatttctat gatctcagga cccggatcag ccaacccatt
    34141 ctcaggcctg agataaaaat caaccggctg atcaaacagg aacagatcgg actccaggaa
    34201 ctggaaaagg aaatggttga aaggaatatc atacgggacg taaaaaaagc ttactatcaa
    34261 tatatccagg ccggggacat gatatccatc ctccgccagg cgctcaccgt actggatgaa
    34321 ggagagcggg tgacccagag cctgattcga aacggcatcg ctctgccttc ggccatcctg
    34381 cgaatccagg cggaacagtc caagctggcc acccaggtct atgctgcgga aaatcagcgc
    34441 ctcaacgcca gggctttttt caactacctg accgggaggc ctgactcgga tagcatccgg
    34501 gccatttctc tgatggccct gcccgaccct gcttccttta cggattcaat ccggaccgaa
    34561 gagatcaaca gcctggaaaa aggcctcgcc attcagcggc tggcccttga tcttgaaaat
    34621 aaatttcatg ccccgaaact tggagcccag gtggatcttg gatcccaaaa cttcaatttt
    34681 aaatgggggg gatacctcct tgcaggtttg cagcttgatg tccccctgta tgacaataaa
    34741 aaaaataaat accgcaagga ggagatcctg gccggcatga aagccgcatc agaaaaaaaa
    34801 gactgggcgg ccaaaagcct ggggctgcaa ttgcagcagg ccaaaaggca aatggcttcc
    34861 gccgtggatc aataccattc ctacagccca atattaaata tgtcccagcg ctattactgg
    34921 gaaatcctgc gcagatacaa agaaggccag gccacccctg ccgaactcgt ggaagcacag
    34981 gcccagatca catcggccca gctccagcaa aacatcgcac tttatgaatc ctggatccgc
    35041 gccgcggaaa tcgaaagcct gattaaaacc caataaaccc aaaccccatg gtttcatacc
    35101 tcttcaattc cccaaaaatg aaaatccatt ctttcctcct ggtcctgcct cttttttttg
    35161 ttttttgcgg cagggaaaaa acagaaccgg cggttcaaat tcatccggac tccctggaaa
    35221 tccataaagt aaaagtagcc cccgtcaccg ggacggaaac caatggcatc atccgggcaa
    35281 ccggtcttct cgtctcggac ctcgagtcca ggccggcctt taaaataggc ggggtgatcg
    35341 aaaaaactta tttcggagaa ggagacaaag tctccaaagg cgccctgctg gccaggctga
    35401 atcaatctga aatttcagcc caggtcaggc aggccaggat ggccgtggaa aagaccagca
    35461 gggattttga ccgggtcaac aacctccatg cagacagtgt ggccaccctg gaacaattgc
    35521 aaaatgccgg gacggccctg gatatggcca aagagtccct gcgtatagcc gaattcaacc
    35581 agcaatttgc cgaagtccgt tcccccgtga gcggcacgat tgtcaaacaa ttgcttcgcc
    35641 agggagagat tgccggaccc ggaatgcctg tctacgccat catgggcaca ggccggcagg
    35701 attggaaaat aaaatccgcg gtatcagaca aggaatgggc ccggataaaa aaaggtgacc
    35761 ctgcagaagt atggtttgaa gcctatcccg gcctgaaatt gcagggcgtg gtcgaccagg
    35821 tagcggagat gagcaatccg ggcacggcca ccctcgatat tgaaatccgc ctgccgggac
    35881 aaaatcagaa actcgcggcc ggattgttgt gcaaggttga aatccgggcg gcagccagga
    35941 tgggttcttt aaaaaccatc ccgatcgagg ccctcgtctc ttcaaaccag ggattgggga
    36001 tcatctatgt catcgaggat ggaaaagcgg tccgtaaaac ggtccgcatt gataaaatac
    36061 tgggtgacca ggtagcggtc agcagcggcc tggaaaaagt cggagaggtg atcaccgccg
    36121 gcgctttcta cctggaggaa ggtcataaag tccagatcat tcaataagcc ggttttttat
    36181 ccttcaaaat tctaaaaatg aacctgactt cattctcggt caaaaactac cagtttacca
    36241 tcattatatt cattcttgcc ctggccctcg ggctgaattc attgctcaac atgccccggg
    36301 gggaggaccc gccattcggt gctcccattt ttgtcgtcct tgccgtctac ccgggaacca
    36361 acccggcgga tatggaacaa ttggtggccg acccgatcga agaggcgctt tacaatctgg
    36421 gggatgtcaa acggatcgtt tccacctgtt ccgacggatt gatgctctgc cagattgatt
    36481 ttaattacgg ggtgaatgtc gataccaaga acaacgacgt caaccgtgaa atgaataaaa
    36541 tccgtgccaa ccttccggaa ggactgctgg aattgcgcgt ggaaagagcc tcctcctcgg
    36601 atgtggtgat ccttcaaagc gctttggttt ccgagacggc caccccccgc gaattgaacg
    36661 acctggccga tgaattgaaa cgcgagcttg aaagggtcaa ggacctgaaa tgggtaaaaa
    36721 tccagggcga accggttgaa agaattgata tccagctcaa gctcgaccgc atggccacca
    36781 tgggcatcgg gatcaaccag gtgatccagg ccgtgaatta caacaatatc aatatcccgg
    36841 gaggcaccat cgacctgggc accaaacggt tcaatatcaa aaccaacagc gattttaaga
    36901 ccgtggacga catccgcaac ctcatcgtgc acgccggcca cgaaggccag ctggtcaggc
    36961 tgcacgaggt ggctgatgta tatcccatcc ttgccgaaca aaatcatata gcccgccaaa
    37021 acgggaagcc ggccatctgg attctatcgg ccctgaaaga caaaaaaaac atcatcaacg
    37081 cgagggaaca gatggaaggc atcctgaaag cctatgaggc gcgcctgccg gaacaggtgc
    37141 agctggtgca cgcttttgac cagtcggtca gtgtacagcg caggctcgga ggcctgaccc
    37201 gtgatttttc catagcgatt tgcctggtct tgctgaccct gctaccactg ggcatgaggg
    37261 cttcttttgt cgtactgatc agcattccgc tctccctggc tattggcctg gccttgttgc
    37321 acctcaccgg atttaccctc aaccagctga gcatcgtagg aatggtcatc gccctgggcc
    37381 tgctggtgga tgattcgatc gtagtggtgg aaaatatcga acggtttatg aggcaaggct
    37441 attccaggaa agaagccgcg atcgtagcca ccaaacaaat attcatcgcg gtattgggct
    37501 gtaccgccac cctgatcttt gctttccttc cgctcgcctt tttacctgaa ggggctggtg
    37561 aatttatccg gagcatgccc atggcggtgt tgttgaccat cctggcttcc ctttttgtag
    37621 ccctgaccat tattccattt ttatccagcc ttttattgca aaaacacgaa cgccccgaag
    37681 gaaatatttt tatgagggct ttcaaaagat acgtcaacga tccttatgcc ggcttgcttc
    37741 gctgggcatt cagacacccg gtacttaccc tcctggggac cgttgtgatt tttgcaggaa
    37801 gcctgatgct ggttccgaaa atcggtttca gcctcttccc caaatcggaa aaaccgatgt
    37861 ttaccatcga catcaaagcc gcctcgggat caagcctcaa ggaaaccaac cggatcgcgc
    37921 gccggattga gcaggacctc attggcaccc cgggggtcgt ctcggtaagc accaatgtgg
    37981 gtaaaggaaa tccaagagtg tactataatg tgttccagca aaactatacc ccgacttatg
    38041 cccagttgct ggtccaggtg ccgtcttcca tggatgtgcc cgccattacc gcgtttacgg
    38101 attctctccg ccggaactac gagggggtac cggatgcgga ggtggaagtc aaaatgttcc
    38161 agcagggcac tcccgttccg gcacccatcg aattcaggtt gatcggcaac aacctggata
    38221 ccctgaaaca ttattcctcc atcctggaag aggtgattgc caataccaaa ggcaccatag
    38281 ccatacgcaa cgatatccgg acgcccaaga ccgacctgag ggtaaatatc gataaggcga
    38341 aagcaggcat gctgggggta ttgcccggtg cagtcgccca gaccgtaaga ctgggcctcg
    38401 cgggcctgga gacgggatcc ctgattgaag aagacggtaa agaaaaagtc atccagatca
    38461 gcctgggcaa accggatgcc cggcaggcgc ttgaagtgtt tgaccagttg tatgtgcagt
    38521 cggtcagcgg cgcccaggtg cccctcaaac aggtggccag tttcagcctg gaggattccc
    38581 ccctgctgat ccagcatatc aacaaggaaa gatataccgc ggtgaccagt tttgtggaaa
    38641 ccggatacaa tactatccag ctgatcaacg acatctccca aaaaacggac cagcttccat
    38701 tgcccgcggg atatcggatc gttgcggccg gggaaaagga aagccgggaa gaatctttcg
    38761 ggggtatggg taccatcgtg ctgatctccg tatttttcct cttcgcgatc ctggtcctcg
    38821 aattcaggac ctttaaaagc accctgatcg tactatccgt cgttccgctt ggcatcgtgg
    38881 gcgccctggc cgctttgtat attgggaatg aaaccctttc attcgtagcc accatcggca
    38941 ttattgccct gatgggcatt gagatcaaga actccatctt gctggtggat tataccaatc
    39001 atttgcgcga gcagggcatg gaactccgcg aagccatcat gaacggggcg gagacccggt
    39061 ttctacccat cctgctcact tcgatgacag ccatcggggg catgatcccc ctggtgcttg
    39121 aaaattcacc cctgatctct ccccttgcca tggtcctgat tggcggtctg atcagttcga
    39181 ccctgctgag ccggatagtg acgccgctat tgtattacct gatcccaccg gcgattgtgg
    39241 tgaaggagga gggatgactc gaagacaggg ggtgtctcca aaataccctc tgtcttaatc
    39301 acttaatcaa aaaaatctca tgtcttccca ttcctatttc gatctcgtct atgaagtcac
    39361 ccgacatatc ccgccgggcc gggtttcgac ctatggcgcc atcgctgatt ttttatccct
    39421 gggttccgcc cgcatggtcg gttgggcatt gaaccactgt catggactga aagacgttcc
    39481 ggcgcaccgg gtggtcaaca gcaagggtga gctaagcggt aggatgcatt tcaaaccgcc
    39541 ttcgtccatg gcggccaggc tgaagaaaga aggggtgaaa gtaaaagttg accgggtagt
    39601 cgactttgaa cgcttattct ggcatccccg ggaaatggca gtggattaga cccctcccat
    39661 cctcaagctt atattttttt taacactttt tccgtgcaac accggccccc ggaaactgtc
    39721 ttttcataaa gtatcctgcg tcttaactat aaaatgattt ctatgaaaaa actattacta
    39781 tttgtgatgt tcctgttttg catggccggc ctgcaggccc agcgggaaga aaccctcctg
    39841 cgaaacatga gtttttccgg tgcctgggga gcctggaaca gttccattgg caaggttgcg
    39901 gatgaaaatg tttacttccg cggaggttac ggaggagtgg aattcggagg cgatttattc
    39961 ctgggctggg gaggataccg ttcctcggac cggttttacg cggaaggcga acggatgcgc
    40021 atgaaatgga atggcccgat ggtcagttac gcccctcatt cataccgggc ggtgcatccg
    40081 gttttcaccc tgatgctggc caacggcaaa ctggaccccg acaacggccc ttttgaccgc
    40141 attttcgtag tccagccttc gctgggcgcc gagcttaatt tattgagatg gtgtcatgtg
    40201 ggcgctcacg gcggttatcg ctttgtcaac aacgtcaacc taaaccaatt caatgatgcg
    40261 gatttttccg ggctatatgg ggaactgact ttgaagtttg ggtttagctg gggtcgctgg
    40321 tgatccccca gcccctcgca tctcgtatct ggtttctcgc ttctcgattg attacctttg
    40381 tctgctatgg cggacatcat caaactccta cctgattcta tcgccaacca gatcgccgcc
    40441 ggtgaggtga ttcagcgccc cgcttccgtg gtcaaggaga tcttggaaaa cgccatcgat
    40501 gcgggggcca cccggttgca cctgctgatc cgggaagccg gcaaaaccct gatccagctc
    40561 atcgacaacg gcagcgggat gtctgaaacc gatgcccgga tggcctttga aaggcatgcc
    40621 acttctaaaa tcagaaaagc cgaagacctg caattcatcc gcaccatggg ttttcgcggc
    40681 gaagccctgg cctccatggc tgcggtcgcc agggtagaac tgaaaacgcg gctccaggac
    40741 cgggagcttg gtaccaggct tcagatcgcc gcttcggtgg tggaaaacca gcagccggtc
    40801 caggcgccgg tgggcaccag ccttgcgatt tccaatttat tctacaatgt tcccgcccgg
    40861 cgcaagtttt tgaaatcgga tgccgtggag ttccgtaata tcctggacga attcgaacga
    40921 atcgccctgg cttaccccga aatcgaactg agcctccacc acaacgatca ggaattgtat
    40981 gctttgccca ggacccagct caaacaaagg atcatcgggg tttttgggaa aaacatcagc
    41041 gacaaactat tgcccatcga agaaaacacc gacctggtca atataggagg atatatcggc
    41101 accgtggatg cctgccggaa aaaaagaggc gagcaatacc tgtttgtcaa ccggaggttc
    41161 atccgaagtt cttatctgca tcacgccgtc cgcgctgctt acgaatccct gatccgcgaa
    41221 gaggaccatc ccttttatgt tttatttctg gatatcgacc cggccaggat tgatgtgaac
    41281 gtccacccca gtaaacatga gatcaaattc ctggatgaac gcatcatata ccaatacctg
    41341 cgcgtcgcct gccggcatgc actcggacaa tactccgagc ccgtgctgga ctttgaacag
    41401 ccggaccgcg ggatggtgca taccttatcc acctcggcac cccctcccgg tctgaaagca
    41461 accatcaacc aagcccacta cagcgaatcg gccatccagc aggactggcg taaaatatat
    41521 gaagggataa aaccggagca aagcggtcaa gctctggtac ttgggagcaa tatgtcccag
    41581 gcgccggccg cgccggaaga gggtgctgat gaaacgttca gcccagcccc cgagaaaacg
    41641 ccctaccaga tccatcagtc ctttatcatc agtcccattg cttcgggttt cctgctgatc
    41701 gaccaggaag cggcccatca gcggatcctt tttgatcaat gcctgaaaaa gctggaaagc
    41761 cagaaaggag acagtcagca gatacttttc cccaccaccc tgcagttttc cggttccgac
    41821 aaagccctct tgctggagct gatgcccggc ctgaaagccc tgggctttga cctggaggaa
    41881 tttgggcaaa acgccttcat tgtgcatggc ttgcccgtgg attggaagga aacggaatcc
    41941 attcaggcat ttattgaatc cctgttaagg caaaccctgg aagaatcaga ccggaaaacc
    42001 aatcaccggg agagactggc ccggcattat gcccgctacc agtcgatccg ggccggccag
    42061 cggctcggcg cggaggagat gcagcacctc atcgaccgtt tgtttgcctg tgaggattcg
    42121 gcctggagtc cttttggagg gcggtgctat acgacctttg acttaggtga gattagaaag
    42181 aagctgaaat agggaatctg ttgcatcatt cttcgtttat atttttttac ctgtttttct
    42241 tacctgcccc tgttttttat tagaaaaaaa gtcaaatgag tatgcttttc ttaattccac
    42301 cttgttccgt tctttcctat catactaatg ccctgttctg tcctttcctt tcacagagcg
    42361 caatattctc cttatatgtt cagatagaag aataagcata agaaataata ccaagagggc
    42421 aactaccatc aatattatta tttggaaggc actcattttt aattaaaatt aattggttta
    42481 tcaatttctt cacaataata acaagtagcc cctaagtact taggtgaaag tctgaggttt
    42541 tgtttttaag gtggcccttt ggagaatgga gaagattggt attctcaata atttttaacc
    42601 caaaacacag ttgtggatac tccatggtag tttggttggg tcaaaggggt gccttaacgt
    42661 atgtcatgat catggacgca ttggtaaaat cacattgtat tttctattga atctcaccag
    42721 gcgtcattta acttgtaagg ataccgcgag agcggatgga attggatgcc aaacttgatt
    42781 gtagtcaaca aatgccaaca tactgatttg acataaatac gaaacccttt cctatcagaa
    42841 cattaagaat ctacttctca ttccctcccc ccaaaatacc tcctgcattc cgtcacctta
    42901 tccgtattga tgatgtccat cccaagggtt ttaaggcggt cccaggatgg cgcttcatcc
    42961 ggggcacccc agagacgaag caatttaccc tgatcatggg cccggttggc aacagttaag
    43021 atctcaccgg gagcctcttt atattttgaa taggaatcgc tgatcagacc gaccctttga
    43081 agcacttcca gggtataaga ttcattgggc cggccatcaa aaaacaacca ttccggccac
    43141 tggagccagg tatccggtga gccccggttg ccgctgatga tgatttgaac tccgtttttt
    43201 cttttgacgg ggtggatcat ttttaagtaa ggttcgatca gctgcaccaa ggctggcagg
    43261 gtggcgccgg cctcggactt gatatcgatc atcagtacgg gagcgtaatc cttgaccttg
    43321 ctgatgcggc cccggttttg tttgaatagt ttttgtatgg gcttgaggta taaatcctcg
    43381 aggcccggtg cggaaaccag ttcttcggtg tcgtgggcta ccaggatttt atcttccacc
    43441 agataaatat ccgcctcaat ggaccaggcc tggttccgga gggcattaaa aagcggctcg
    43501 gcctggtggt aatcgttgtg cgaatggatg cgaacctcct ggccgagggc cagtcccgtc
    43561 cacaggaaaa agcaggcccg gatcagaaaa gaccgacccg gccggtatgg gatttgcgcc
    43621 tgcaggattc tcagtgttaa cgcgtcaggg gaaaccggtt attatttttt acccttcgcc
    43681 gcataataaa taccggcata caaatggttg aggaaagccg gatccctgta caccgcagga
    43741 acatgaccta gagcggtata aaaggccctt cccccgtcat attcgtgata ccaggccacc
    43801 gggtggaaac ccatgccctc tcccttgttc tggccccact ggactttggt attataggtg
    43861 ttttcgtcca cggccaggat ataattcagg cccgttgttt tttccgggcc gaattcatac
    43921 cattcatcgg tccagggatt gctttcccgg aaaagttcaa ggccggggaa tttattggtg
    43981 gtgaaacgaa gctgggcggt ttgggtcggg ggatggatct tgaacatgcg gccaaccatt
    44041 ttggtatacc attcccattc gtattcggta tcggaggcgc tgtggacccc tacaaatcct
    44101 ttgccggccc ggataaattt ttccatggcc gcctgctggc tttcattgag gatatcgcct
    44161 gtcgtgttca ggaatatgat cacatcgtaa cgggcgaggt tgttgtcggt gaagcgtccc
    44221 gggtcttcat gccattccac atcaaaaaca tgacggtcgg ccagctcctt tacggccgta
    44281 accccatcgt ggatggattc atgatgaaac ccgttggtct tggtaaataa aagggccctg
    44341 aattgttttt gggccagtcc catagaagaa acgaaacagc agaaaaggag aaggagttga
    44401 aattttttca tgatgtacac caatgttttt agtggaatga aagtactgat tttttgaaat
    44461 cctgtttgga taatggagac cgggatggtg cttttgtttc aaaaccggct taaaatgtat
    44521 tgggtcgctt ccggattttg agaattattc cttttcatgg cttgcgcact ttttgtagtc
    44581 cttaaatttg gataggattg gtaggattaa taggattggt aggattggta ggattggtag
    44641 gattaatagg attagtagga ttggtaggat tggtaggatt ggtataatta gtaagattag
    44701 tatgtttagt atgtttaaaa atggtttccc tctttttcgg gtggttttag ccttgctttc
    44761 tcctttcatt tccctgaagt cctttgccca ggatgcggtc agtttggccc aggtttataa
    44821 agattatttc ctgatcgggg ccgccgtcac tccccagtcc ctaaaaacgg atgaagccgg
    44881 gctgatcctg agccagttca acagcctgac cgctgaaaac gccatgaaaa tgggacccat
    44941 tcatccgaag gaaaacgaat accaatgggc cggcggggat tccattgccg cctttgccca
    45001 aaggcacggc ctgaaaatga ggggccacac gctttgctgg cacaaccagg tggccccctg
    45061 gatgtttaag acggaaaccg gggatacggt caccaaagaa atattgatgc aaaggctcaa
    45121 ggaccatata accaccgtag tcagccgcta taaggaccag gtctatgcct gggacgtggt
    45181 caacgaagtg atttcagatt ccccgggaga attttaccgt ccatccatct ggtacaggat
    45241 ctgcggggag gaattcatta ccaaagcttt tgagtgggcc catgccgccg accccgacgc
    45301 cctgctcttt tacaacgact acaatgaaac ccacccggtg aaacgcgaaa aaatcttcca
    45361 gcttgttaaa actctcaaag aagcaggggt ccccatccat ggcgtcggac tgcaaggcca
    45421 ttggtccata tatgaaccca ccgccgaagt attggaacaa accctggacc gcttctcgca
    45481 actgggactc caggtccagg tcactgaact ggacatctcc gtttatgcca aaggccaggg
    45541 acggcgggaa ccctggccca aagacagctt gagcgtgttt accccgcaaa tggaaagttt
    45601 gcaaatcgag cggtataaaa tttgttttga cctgttcagg aaataccgcc ataccctgaa
    45661 ttccgtgacc ttttggaaca tctcggaccg gagaagctgg ctggacaact ttccggttcg
    45721 cggaagaaaa gattatcccc tgctctttga caaagacctg aaacccaaaa aagcgttctg
    45781 gccggttgcg gattttcaaa aacagggagg ttaaccggac tttgtcgaag aacttctact
    45841 tacgctccct tcgcccttcg ccttttacct ttcgccttct acttttnnnn nnnnnnnnnn
    45901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45961 nnnnnnnnnn nnnnnnnnnn nnnnnngctc ccttcgccct tcgcctttta cctttcgcct
    46021 tctacttttt gctcccttcg cccttcaccc ttttgctcct ttcgccattg actccctgaa
    46081 atcctgcaaa atcgcttcct tattaaaatt ttcccaaatc gtaatttttc ttgaattgtc
    46141 cggccgttcc acccattgac cgtcgaccag cagaggtgca ggaataacca cttttcccgc
    46201 ccaggtactg tccttaacca cagccaccgc tgccatatca aaaagggcgc gggagggggg
    46261 atcactgtgg taatcgatgt gctcaaacag gctcaccgaa taatccccga agcaacgata
    46321 ggtcccgcca tgccttccgg tcaccggagt tttgactttc ggacccttgc ccggcataaa
    46381 ttcattgatt tccttttgcg tgattttcac cgcatcggtg ccgcttggtt tgccgtaacg
    46441 caccgtgacg atctcaaaat ccaccggaag gttcaggata aaattgaggg ccgcggtgtc
    46501 gttgttcagg ttgtattcgc cgggttcggg ataattggag cccagccaga ccaggcggat
    46561 gcgcgacacc aggcccgggt ccttcataaa agccagcgcc agggtggtca gttttccgac
    46621 ggcgatgatc gtcagttttt tggtgggttc ctcccgggca cggtcggaaa taaattcaac
    46681 cgcatccttg ccatcgtaag ttacggaatc cagttggggc aggatttcaa tgaaagaagc
    46741 atctgcccct tttaataagg gcaggctatc ctcattgcag agccggagta tacgttccgc
    46801 ttcctggtat tgctgatcga tcccgccgcc gctgcgggtg gcattgacgg tgacgccttc
    46861 cacctcaaac cggtcctggt taaacaacag gtaagccagg gcatgctggt cgtccagttc
    46921 attgttggca tcggtgtcaa aaatgacccg gatcctggag gaagaaactt cttctttgtt
    46981 tttacaggaa aaaatatgga gcgcgaaggg cagcaccaaa accaatttca gcggcatttt
    47041 cacctgataa aggtaggcat tgcctggttt ggttttatcc tcaggcctgc cggggtcctt
    47101 atttgcccat caggtatttt ttaggcgtca cgccgaaatg ttttttaaag gcggcgataa
    47161 aatgactggg attggaataa cccagggcat aagccacttc atttacatgg ggggaggatg
    47221 aatccatcag ccggcgggcc tgctccattt tacgggccag gagataacca tatacggtat
    47281 tgccgtaaat ttccttgaag ccggatttga gctggtattc aggaatggac acctgttggg
    47341 ccaggttttc gatggtaggc gggtttttca ggtcccggat caggatctcc ttggcatcct
    47401 tgatcttttt cagcacgttg acatcattga gaaagggaca ggaactgttg tcgctttctt
    47461 tgtccgaaaa ataaagactg acgagttcaa ggagcttggc gtaataaaag gtatgttcca
    47521 gggatttgtt gagttggacc ctggccaggg cggacagggt gagtaaaata gcctggctca
    47581 tgggtttgtc ctggtaaaat ttttgctgga tctgttcgct gtgcaggatg ggcaattcat
    47641 cctgtgaaaa aaggcggtgg agtttatcca ggctgataaa aagggtgtac aactggaccc
    47701 cgggtttcaa ttccaataca taggcatggt cctgcaaggg gttgtataaa aaggttacgc
    47761 tgtgctcgtc gatctggcgg atataggggc caaaatgaag gcggggatgg ccttccaggc
    47821 caaaataaaa atgcacccgg tctttacgta aaaaggaagg caccgacacc gcctggtctg
    47881 aatgattttc ccaacggtca aagaataccc cttcatcctg gaaaagcgaa gtctggtttt
    47941 cgcttttaac ggtatttttt tcagtaaggt ccggcatatt ttttataaaa attttattca
    48001 aaaatacaat aaattgaatt cctgagcatt atacaagaat agattacaag caaaaattac
    48061 ctttcggggt aataaaattt ccttttaggg tactttatga ttgatcctcc cttttaattt
    48121 gcaccgtgat tttccgaaac cctgtttttc tcctgtcttt ggtatcggtt tgcggcctgg
    48181 tccgggccca gaccgtggag gacagctttt ccattcaaaa catacccgag atcgtagtca
    48241 ccggtgaatt tgagcccctt gaagccaaaa gatcggtgta ccaggtcagg accattggtc
    48301 aggaagccat ccgtgcccgg ggagccaccc ggctgcagga cgtattgacg accgaaacca
    48361 atatccgttt ttcccaggac ctggctcttg ggggctccaa tttgtccatg cagggactgg
    48421 ccgggcaaaa tgtaaaaatc ctgatcgacg gaatacccat ggttggccgc cagggcagtt
    48481 ccaatgaaat caacatcaac caaatcgaca tcaacagcat ccagcggatt gagattgtgg
    48541 aaggtccgat gtcagtcatt tacggcgccg atgccctggc cggcgtcatc aacatcatta
    48601 ccaaaaaagc ggtggaaggg agggcagacc tctccctcag gatccatgaa gagacggtgg
    48661 gaaaggaata cggctggccc caagggctgc acaatcaaag cctgaacggg ggatataaaa
    48721 agggggactg gtattttaaa ggggaccttt cccgcaatgc ttttgggggc tggcagggta
    48781 caggcatggg cagggatctt cagtggcatc ccaaaaccca atacctcgcg ggcggagtag
    48841 ccggcctgga gaaaactaaa accaccgctc atttccggct tgacctgatg cacgaagatg
    48901 tttacaaccc cggagccgct gaagccggcc aagccatcga tcaaaattac cggacgaccc
    48961 ggtggatgaa ccaggttcaa tggaaccata ccctgggcta caggttcaaa ttgaacggtt
    49021 ctgtggcttt cactcatttc cagcgaaaaa cccaaaccac cctttttaac gaagcgactg
    49081 gcgaccggcg tttgtccctg gcccctgatt cccaggatac cacttttttt cgtggtttca
    49141 ccacccgttt actgttgccc tggaaaatca attccgcatt ttccctgttg cccgggctgg
    49201 atatcaattt tgaatccggc aagggggggc gcatcgaacc cggtacccat accattgggg
    49261 attacggcct tatcctcacc ggggaatgga aaggcggtga aaaacttcaa gtccggcctg
    49321 gtctccgttt tacccataat tcagcctacc aggcccctcc cttattgcct tccctcaacc
    49381 tccggtgggt ttttgcaaaa caacaggatc tccgcctggc ctatggcagg ggattccggg
    49441 cgccaagctt aagagaactt tatttcagct tttttgattc caaccattcg atcgtaggga
    49501 atacagaact taatgcagaa ctgtcccaga gttttaccgc atcctggaac tggacggttt
    49561 ggcagaagga aaccagccaa ttgactgccg gcctcactgg cttttacaat gatgtgaata
    49621 atatgatcgg ctatggtcaa aaaccaggca acgccctgtt gatcagttat attaatatcg
    49681 atcgttttaa aaccaagggg ctcaccgcaa acatgagttg gaaaaccccc accctccatt
    49741 ttaccctcaa cggcacctat accggacggt acaaccaggc aggtcttaat gaagccattt
    49801 tggaagaatt tacctggtct ccggaaattg caggcacctt gacttattct ctggctcgct
    49861 ggggaacttc attttccctg tttcacaaat acaccggccg gaccccttac ttcgagatca
    49921 tttccgaagg caatgagcaa aaactccgtc aggcagaagt agaggcctat tcctggaccg
    49981 acctgagcct gaaaaaagaa ttgaaaaatt tcgggtcgct gactttgggg atgagaaacc
    50041 tgttcaacgt gatcaatatc aacaccgcca ccctcaacgg taccggggtg cactccgggg
    50101 gggcaaaccg gccggtgggg tatggcaggt ctttctttgc cactattcat tataccatca
    50161 taaaataatc tcaatatgtc ctttagttca aaaaagcatt taattctttt ttccttgttt
    50221 ttatttgttg ccctgcaagc ctgcaaagac gaccccgctt tgcctgacaa tctcatcggg
    50281 tttgaaagca accagctggg tttcacctcc actgaaaatg aaataaccct gcaggtaaaa
    50341 tttacttacc ctgcttatga gtccggtacg gttctgatca atatcctggc cgaaggcctg
    50401 gtctatgaca ctgattttac cacagaaccg gccaccgttt ccaacgccct ttcgattcca
    50461 tttctcgccg gggagaccgc caaatcaatt aaagtgatca aaaaacccgg tctcctggta
    50521 gacggggatg aaaacatcgg ttttaccctg gcctcagccc cggcaggaac aatactgggt
    50581 cccaatccca gtcttaaact gaccttcgcc gaaatcattt ccgcctccgg cgcggcgacg
    50641 gtggatggag gaggcgccac ttatccaaac aaggttttta tcgatctcag cgccaacagg
    50701 caagtcgcca ttccacgcac cggctgggat cttggatttg ccgccgggaa tgagttcagg
    50761 gtcatcctca atgcatccaa tgggatgatg gcgatcgcta cggataaaaa cagtatggca
    50821 gcggttacgg ctgcagacac cgcaggtctg tccacccgcc tgtctctgaa tgccgttttt
    50881 gccgccatca acctcgatcc ggtccccgac tgggtgccca acgccatcac gtggatcgat
    50941 gatccctcgg gcaacctcag caaaaccgcc ctcgccgaaa tcaacacgac cgactcggaa
    51001 aataaggtct atatcgtcaa tctcggcaat ggaccaggca ctcccgctcc ggaactcgga
    51061 tggaaaaaaa tcagggtgct gcgcaaggcc gccgggtata ccctgcaaca cgccgacatc
    51121 ggttccgcca cctttacgga agtttccatt accaaggaca ataatttcaa tttccaatat
    51181 gtcgattttg agaaaggact tgttcaggtt gaagctcaga aagacaaatg ggatatcagc
    51241 tggacgggct ttaccaacag caccaatttc ggttcaggcc cggttcccta ttattttcag
    51301 gacatcgtat tgcaaaacac ccaccaggta caaaccgccc aggtcctgac cagcgccatt
    51361 gcctatgagg ccttttcaga aacgaacctg gccaccctgg attttggaac ccaatcccag
    51421 gttaaaatcg ggcccaactg gagatccgga gggggtcccg gtgccgcacc ggccattcgc
    51481 acggaccgtt tttatattat caaagatgct gccggcaatg tgtataaact ccggttcacg
    51541 gccctcacct ccgatgggga aaggggtaag ccgaaatatg agtatagttt ggtgaaaaaa
    51601 ggataatagg ataataggat ttataggatt tataggattt ataggattgt aaaggtttaa
    51661 aaaatcctac aatcctacca atcctaccaa tcctactaat cctactaatc ctactaatcc
    51721 tactaatcct ataatcccag taacttgcct gtaataaaaa acgcaggcct gagaatctgg
    51781 atcttaacgg ttttatggat gagcggcccc ctgctggcag cccaggacaa tcttgtgccc
    51841 aatggggatt ttgaagagga acttccatgt ccggggaacc tggctaccct ggctcattgg
    51901 tttgtgcccc aaaacagcgt cgtagacctg aataacctct gcccttatat cgattggtgg
    51961 aggtttatcc gcgacaaaaa agaagggatc aatggcagcc agtgcgggta tgcggagact
    52021 tattaccgcg gctttgccga tgataatatc tacagcggaa ggatatacct cgccgccaaa
    52081 ttgcggaaac ccctggaagc gggtaaagaa tactattttg aaatgcagtt ccgggcggta
    52141 gatacttttc cgaataaaaa gctggtcaat acggtgttta ccaacagcca ggacatcgca
    52201 ttcaccaaag agcccccgtt gtttgatttt aatatcccca ggaattatat ggagctcaaa
    52261 cccgtggtcg ccagtccctt gtataccgat tacgagtggc acaaactgag ccattgtttt
    52321 gtcgcggatg gaaccgagca ttacctggtg atcggcaatt ttaaaaacga tgccaatacc
    52381 ctcacggtga gtaccgggaa aaggaacccc aatttcccta acgggctgat cgccaattat
    52441 gccatcgatg aggtggtcct cacggccatg gaaatcccat taacggatac cgccatgtgt
    52501 gtgggcgata ccctcagcct gaacgtcggt aaatcagtgc cccgggccct ggcctaccgg
    52561 tggcaggatg gaagcacccg gccggtttac cctgcataca aacaggaaac ggtccgggtc
    52621 gaaatgattt attccgccca gtgccggcgg gacaaacaaa tccgggtaac ccctttcagc
    52681 agggaatttc cagtccagga tcaattttgg gatacggtga tttgcgaagg gcaatttacc
    52741 cccgtgcttg ccgggatgca tcttttggga gaaaatatac gctgggagga tggccgttcg
    52801 gatccaatgc gcatcatcca tgatccggga acctatactg ccgatctcag tcaccgttgt
    52861 tttaacagca tccggcaggt ctttaaagtc gaagtcgaat cctgcctggc cggcatttat
    52921 ctggccaatc tttttacccc gaacggcgat ggaaaaaacg atgaattcag gcccgttttt
    52981 aaacccgact tcccggctat ccaatcttat gaactgagca tctacaaccg ttggggagaa
    53041 ttggtgttta tatcccggaa cctggaaacc ggctgggatg gccgaaggtt caaccaaccc
    53101 gttccgcccg gggtctatct gtattccctc cgcgtgacca cccaaagccg gagcggtcaa
    53161 cgcacgcacc ggaaatccgg ggatgtgact ttagtgtatt gatttacttc ccctcctccc
    53221 gcctactgat cagaatcatc acccttctat tcaattattt tattgaatac ttgatttagg
    53281 ggtatttttg tacaaacctt taagatatgg tctccgaatc cctgaaagaa aaaatttaca
    53341 aagaactgtc ccggggcacc cgggcccttt caaatcccaa gcgaatggaa ttattggatc
    53401 tcctttccaa taaagcgtgg tctgtggaag agctttccaa ggagttatcc atgtcggtgg
    53461 ccaatacctc ccagcatctc caggtcctga agtccgccat gcttgtgaaa acccggcgtg
    53521 aaggtaattt tagattttac accatctccg atgaccgggt actgcgtctg gtagctgcgg
    53581 tcaaagagct gggatttagc cagtatgccg aagtcgagaa aataataaac gattttaaag
    53641 ccgataaaaa tattttggat tcttttaccc tagacgattt gattaggagg gcaagaacgg
    53701 aaaaaatcct gctgatcgac gttcggccaa aggatgaata cctggctggg catattccgg
    53761 aggcggtttc ttttcccctt tccaaattaa aaaaaaggat tcatgaactg cccaagggca
    53821 agaccatagt ggcctattgc cgaggcccgc tttgtgtcat ggccgtggaa gccgtaaaaa
    53881 tattacaggg gaaaaaaatc aaggcggtcc gaatggaaga cggatacgtg gaatggaaat
    53941 taaagcagtt ggaaaccatc ggatcgggtc cggcttttaa aaatggatga ttatcagtca
    54001 atatcgtttt atgcacttcg gaccggaccg gatttcaatt tcttcaatat ccttaaaatg
    54061 aaaattttaa ttattatcaa tgatccccct tacggcacgg aaaagcttta caatgccatg
    54121 cgcttgacgc atcaactggt caaagaccat cccgagattg aattgcgagt attcctgatg
    54181 gcggatgccg ccaccgccgc gctgaccaac caggtgaccc caaatggtta ttacaatgtg
    54241 gaaagaatgc tgaagttggc cctttcaaaa ggagtgaaaa taaaaatatg cgggtcctgc
    54301 gcggaagcca ggggtataaa taagctcccc ctgatcgaag ggactgaatt aagcaatatg
    54361 tctgaactgg cccattgggt cgttgacagc gacaaggttt tgacttttta aatcccctgc
    54421 cccccagcat ctgaatcgaa ataaacaaaa caaccgtgga acgcagaata ccaatagata
    54481 ccggcactct cctgacctgg cttaaagaag gcaaaccgag gcgccgtatc atgattctaa
    54541 atttcaatag aaggcgttaa gcaggtttta tggagcatat caggttgggc ttgaaagaaa
    54601 actggaggca gtttaccctg ctggtgatca tcaatggttt tgtcggcggc atggtcggcc
    54661 tggagcgatc gatcctgcca aagattgctg aacaggaatt tgaaattgcc gcaaagacgg
    54721 cgattctttc gtttatcgtc gtatttggga tcgtaaaggc cctcaccaat tattataccg
    54781 gggtcctggc caaccgattt ggcaggaaaa aactgctcat cgcgggctgg gtcgctggac
    54841 tacccatccc ttttatgcta atgtttgccc cggactggaa ctgggtcatc gctgccaata
    54901 tcctccttgg gatcaaccag gggctggcct ggagcagtac ggtggtcatg aagatcgacc
    54961 tggtcggtga aaaacaaaga ggttttgcca tgggcctgaa tgaatttgca ggctacctct
    55021 ccctggccct ggtcgctttt atcaccggct ggattgccgg ggagtacggc ctcaggcctt
    55081 atccgtttta taccggcatt gtattggttg ggctcggcct gctggccagt atattcctgg
    55141 tcaaagacac ccggcatcat gtggaccggg aaaaactgac cagtaccctt ccggccctga
    55201 ggaatgtatt ttgggatacc acctggagag ataaaaacct gggctccgta acccaggcgg
    55261 ggctcatcaa caacctgaat gacgggatgg cctggggcct cctcccgatc ctcctggccg
    55321 gtaaagggtt taccctcgga gaaataggcc tggtgaccgc ggtttatccc gctgtctggg
    55381 gtttgggcca attgatcacc ggcgccatgg ccgaccggtt ttgtaaaaaa aacatgcttt
    55441 acctgggcat gtttttgcag tccctggctt tgtttttcct ggtcatggcc cataccctga
    55501 cccactatat ttttctgtcc tctcttttag gttggggcac cgccctggtt taccccacct
    55561 tcctcgctac ggtggcggaa aacaccaatc cggaagaccg ggcaaaaagt attggcatat
    55621 tccggctatg gagggatttg ggttatgcga ttggcgccat gctcacaggg atcgtcgcgg
    55681 atttggtcaa tatcaatatg tccatcctcc ttatcggaac cctgactttc gcatccggct
    55741 gtatcctatt attccggatg aagtgcagga ccgtaatccg atcggagatc tcataatgtt
    55801 caggatcatt catcaagctt tctccgcgga aaccggtccg ccggcagctt taaaccttaa
    55861 acctttttct tccagggcca ggcgcaattc aatcatggcc ttgttgccta taccgtgcag
    55921 gctcaggatt tcctgttccg tatattttga aagctgcgaa agctccagga tcccctttgc
    55981 ttcaagggcc cgccgggcag gtgcgctgat tttcgacagg aaaccctccc tgggcctggc
    56041 taatgatgca cataccgggc aaaccgggca gtcgctgctt ttattaaaaa catggcccct
    56101 cgggcaggtt tttttctttt ttccggcgat tgacgtcatg gattttaagt cctgtttttc
    56161 catgttttcc tttacccgga acaggatcat ttccctcagt aacttcaaag gcaagggccc
    56221 atgtaaagga agatggacag cccctttgga acagacatag ggtgcaagag cggttttgaa
    56281 agcctgaatg gcgccggcgc ccggataaaa tccaatgtgc cggtcatagg ctgcaaaccg
    56341 gaccagcttg ccgtgatacc gataggccgg aatataaaag gagatgcctt cccgggcttc
    56401 cggcgcagcc ttaagaatga cctttctgat ttgttcaaga cggatccgga tctccgccgg
    56461 gaaagcggaa aggtagtcat ccaccgtttc ggctccgata ttctgcacgg gctttatttt
    56521 ttcttcccgg atttttgttt gttctcaacc accctgaacc ggacgatccg ggcaatgaga
    56581 tcatagggta tgggctggtc cagtggaaat tgtacggatc cttttgcgcc cttataggcg
    56641 gacaattccc cggcaaaggc gctcatgccg gaaggggtag gataaaaccc gatatgattt
    56701 ttgaaggcgg caaaatgcac gaggtttccg ttgagggtaa aagtggggat ctggtaattg
    56761 atggtttccg tcgcttcggg ggccgcatcc cggacggtcg acctaatttt attcaatatc
    56821 acccggactt cccctgaaaa tcctgcaatg tattcgtcaa tggtcaccgg cctggagttc
    56881 attggatatc tttttttaca aatttgaaaa ataaatccct tttcccctga aggaaacccg
    56941 acattcagga gggggtattc cgccgatgtc acccgaacag tcagagaccg gagtcttgat
    57001 cccagtcttt tcttaccaag cggaaaacca gacaatcatc ctgaccaaac cgccttttct
    57061 ccagcaaccg gaatcccaat ctttcataaa accggtgtgc tcggacgttg ctttccaggg
    57121 gatccacaag gatggcgtta atcccggcgt tttcaaaaat tcgggcaatg gtctgcttca
    57181 tcatttccgt acccaatccc ttgcccaggt ccgcttgttc gcccagccag atatcaatgg
    57241 cgcgcaatcc ccggggcacc tccccccaat aatgactttc ttccagggcc ggatcgatga
    57301 tctggaggaa acccacaggc tttccatctg attcggcgat cagctgttct ctccattcgg
    57361 gttcacgctg cagttcatac tcccagttcc agtcgtcgtt gggatcggat tccagcacat
    57421 gctcctgttt gtcccaatac ctgagtaatt ccaaatcctc cagggaagct tttcggagtt
    57481 tgattctttt ttccataatg ggtcatccat tgaacttttt tcttcttgtg aagaggggtt
    57541 taacccaaat cggaaatcta tttgcatggt ttgggtttac agcccctgtc ccagcgccac
    57601 caccaggccg ttgataatcc tgggcataaa ataggtgctt ttcggaggaa ggattttttt
    57661 attgctcagg atttcccggg gagtaagaga aggtaagatg aaaatatatt tctgagggta
    57721 ttgttccatc agtgttttta aaacctccac atcatacgtt ttttcgaaat aggagattct
    57781 cgggctccgg gtttcatccc ggatttcaaa gacctcattc agaatttttt ccgaaaaaag
    57841 caaaggcagc gcaacccttc cggcccttgc ccggatccgg aaccggtaaa gttgatgatc
    57901 atgggccatg tacaaaaaat cctttttatt cagcaattcc tgcacgggtt cccattgctc
    57961 cgttgcctgt ttcagtcgct gtgcaatctt ttgagcgctg ctgcttttca ggggatccac
    58021 gacccggaaa aagcccgata ttttcaactg gtcgggcgta aaatagaccg tcaggattcg
    58081 ctgatgggcc cagccgcccg ccctggaata caataaagcc gaggtggcaa aacggtgatg
    58141 gccgtccgca atcaccgcgg tacgcaccga ttgtttgtat tgacggctaa gacttgagat
    58201 cctgcgggga tcgtccactt tccaaagggt atggatcacg gttttatccg ggaaacggat
    58261 tttcagcagg ggttttgaac cggatttaac ttcccggtac aagtcgttca acctggcatg
    58321 agcagggatc atcagggcgg ccggtttgat catggctctt ctttcgataa acaactgatg
    58381 atgcagtttt tgttttttat ccagggtgcg ttcgtgtttt cggatatgct ggttgagata
    58441 gtcccggata tccgtcatgg cgatcagtcc cgtggaggtc gtttgccctt ccttgatttc
    58501 caaaataaaa aaagcctccc ccggtaccgg tttgaatacc cctttccgga atgctttata
    58561 aaaatcttcc cggattccct gataaaaaag atcatcaaat tttacctttt tcagatcggg
    58621 gatgtgggca tggaacggaa aaatttgcat aaggaaaccg ggttttcttg gcgtacccaa
    58681 gggtgtttat tcaattcctg ttttcgggac gggcttggta caccaagata taaaattctg
    58741 accatcactt taattcaccc cttgccctcc taaatcccgg taacttttca cacaaaagtc
    58801 ccgggttcct ttcaagtaac caaaatcaat gaatgctgcc cacgttaacg gtgcccttca
    58861 cgggaaacct aaagaaaagg caatttcacc acctccgcat ccagcatcct ttcccctgct
    58921 ttgatccgga tcagagaacc cggtgtggaa caggaggtct ccacataacc catgccgatg
    58981 ggataaccca ggctgggaga aatggtccct gaggtcacgg ttccgatcgg caagccttct
    59041 gaatccagaa tggaataatg ctgcctggga accctgcggt cattgacttt aaatccaacc
    59101 agctttttct tcaccccatc ctttttttgt tgagcgaaaa tttcagaaga ggaaaagggt
    59161 cctttggaca gtttggtgat ccagcctaag ccggcctcga ggggtgaagt agtatcgtcg
    59221 atatcatggc cataaaggca atatcctttc tccagcctca gggtatcccg ggctcccaaa
    59281 cccaccggtt ggatatccaa ggatttaccg gagtcaaata tggcctgcca gagggggatc
    59341 gcatcctttt catcaaaata caattcaaac ccgccggccc cggtatatcc cgtggccgag
    59401 atcaaaacat tatccacccc ggcaaactga cccttggtaa aggtataata ggccatttct
    59461 tcaaggctta tcgaagtaag cggttgcaaa gcccgggccg ccaggggtcc ctggacggcg
    59521 agcagggcgg tctggtcgga gatattgatc agccgggtgt caaaagtatt ggacgcattg
    59581 atccaggccc agtccttgtc gatattgccg gcgttgacca ccagcataaa ggcctgttct
    59641 ccttcggcgc attggtcctc gtccaggcgg taaaccagca ggtcgtcgac gattcctccc
    59701 tccaggttgg gcaggcagct gtactgcgcc tgtccgatct ccagttttga ggcatcgttg
    59761 gtggtcaggg actggatcag gttcagggct tcttttcctt tcacgataaa ttcgcccata
    59821 tgggatacgt caaacacccc caccttattc cttacctgca aatgctcttc cgtgatccca
    59881 cggagataat aaatcggcat gttaaagcct gcaaattcgc ccattctagc ccccaggctc
    59941 ttatggattt ctgccagcgc gagttctttc attcggtgat attgatgcgt tgtattttat
    60001 ctcccggctg gacctggttg accacatcca tcccggaaac cacttcggcg aaaaccgtat
    60061 aattgccatc gaggtgagga gtcgggctgt gggtgatgaa aaactggcaa ctttccgtat
    60121 gcggtccgct ggaagccatg cccacgaggc cggctttatt ataatataca ggcggggttt
    60181 ccgtgcgaat gctgtattcc atcgaaccaa agccgtctcc gcgcggacat ccgccctgga
    60241 tgacaaaatt gggaacgatc cggtggaaag ttttgccgtt gtaaaattca ttacgggcaa
    60301 ggttgacaaa attgatcacg gtagccggtg ccagggcatt cagcaaacga aggcgtatgg
    60361 tccccttatc cgtgtggata tccgcaaaag caggggccgc ggaggaggta aaatcagtca
    60421 aagacagttt tttataatta tccatccggg caagggggct tgcagtccct ttaagactat
    60481 tgagggtttt acccagcatg tactgggcat agggattggc ggccaggctg gatttgattt
    60541 gttccagggt gggaatgtcc accagggttt tcaaaccgga agaaccgtcg cgggccagag
    60601 attccacgat ttcaagggcc gcttcatcgc ccgtggaaag atgaccggta atataagcca
    60661 gtatttcttt ttttacccct tctccgttgc caaaggcgct gctgaaatat ttgcttttca
    60721 ggatttcgat gcccgcttcc attaccgccg aatgaatggc gggcagcgcc gattgcctgc
    60781 cggtttcaag gataaaggag taatttttag gatcggtggc aagggcgcgg aggcaagccg
    60841 tcttttcgtc gatgcctgcg gcacggctca ggatcgattt gagttcagta ttaatattcc
    60901 ctttagtgat tccgtaggca taggaataat tttcgttggc aatgccaagc agggggactt
    60961 tcacctgcca gggcaggttt tccctggcga tattccggta gtcgccggcg tcggctacgc
    61021 gcccgaaatt gtccaggtat tccagggctg tccgtgcgat gctgatgtcc ttgtttctaa
    61081 gagcggccag cataatgttt ttggtctgcg aataatcata gaggttcatc gccctgagcg
    61141 cattgcatac catacgggta tccgtatccg tccggatgat ttcaaccaag gcgttccagg
    61201 cgccttcgga ttttgtttta cccagggcga gaaccagggg aagccggatt tcctgaaccg
    61261 attccccctg caggagggcc agcaattgat ctgcctgccc gctaaggtcc aggtccttgg
    61321 ccctggacag gtaatgcgct gccagcatcc ggacttctcc gggataggat ttgtcggcga
    61381 ggtatttcaa tgccgtggcg gtcccggccg gatccgtcat gcccctcagc atataacggt
    61441 aaatgccgcg catctggccg agcaagagaa gggtatccgt aggctcatag gtttccacgg
    61501 tagccatggc ctgtaaaaag tccggggtgc ccagccttcc aaccgcctca agaatggaac
    61561 tgttgatcgg ggagttgatc cctgaagtat cgtattgccg gaaggcattc agtagggcat
    61621 cctggctgtg ggtggccccc atgaggccca ggctgtatgc cgcagcagaa gcaacgtgga
    61681 cattggcatc gtgcaggagg tgagccaagg tgtcggccat gctgctgtcg cggacggagc
    61741 ccagggcaag ggcggcggca taacggatgg atggaccagg tgcgctgaaa taacttgcaa
    61801 ggacaaccag gtcctggtcg ttttgtgcct tcagtacgga agcgaatacc gaatcgcgga
    61861 ggtccagttc aaccggggtg atatcgatgg catcggaagc cgcgccctcc tgcttgaccg
    61921 gaacgcagga ggaggcaaag aaggcaaaaa ccaggagatt tttcaacaag aatttcatga
    61981 ggaacaactg gatttaacgg aaaaccaatc cggatatacc accccttaac aagggatcaa
    62041 aggtagcgaa tcgcacttaa ttcttccctc ccggtaaacc gaatttgatc aaataaaaaa
    62101 acctttgatc tttaacgact caaaggtttt tctgagtaaa ggaatctttc tttgcttcag
    62161 ctatgggggg cggaagtctg catacgggca tattccgtga tcttgcggat gccggataaa
    62221 gaaggacgga tccggaggtt tttgatttga ttcagaaatt ccctgacctg ctgatcttct
    62281 tcggccgttt tattaaacgg atccggcgaa gccccaaaac caataacagt tgaaaccagt
    62341 tgattcttta gtgtaatgtt ttttgccata gactttattt tgtaatacaa tgcttttcac
    62401 atgtattaaa acttgagccc cgggcgaatt attgtaaatc cgggtatctt tcaaatgtta
    62461 attaggcaag tatttgtttt tcagacaaat atacattaat cggtcacttt ataccaggtc
    62521 tgtgtccgat agattccaga ccagtgggtc cccctgacct tcagctcatc cggcttgccg
    62581 gcctcgatcc agatcgtgca tttgtaatca tttccggtca ccgggtcata gatgcggcca
    62641 tccccatact gatccctgat tttccggagc ccctcgatga ccaccatgcc gagcagggcc
    62701 tgattctttc gtggaccctt gcagacttcg cagatggcat cgggtttatc cttcaacagt
    62761 ttggtgatct tgccgaagag caagccggtt tcttcataaa tacgcacata ggactttgat
    62821 tgcttgtccc ggtcatccac tgtcctccag gtgccggtca tcgtttgagc gcggaccggg
    62881 agccagctca tggaaaacaa aagaattaca aaggctatta taacattatt cataaaggaa
    62941 gacaaagaaa cgggaaaatc aattcacgct ccactttacg gtcttcactc cccgcgcgcc
    63001 ggccacccgg atcaggtaca taccgggaga aaccaaactg ggaagttcaa tcacctgggt
    63061 tccgggaatg acatgcaact ggtgtttcca gggcagcggt ttcccgtcaa ccccgaaaat
    63121 cccaatctcc atccggtctt cacggccagc ggatatttcc aaaaaggcca agtcccggga
    63181 aggatttgga agaactcgaa gggcatgaat ccccgcgtcg acgtatttca ccggagtggt
    63241 cacgtccagt ttccggtccg tatacacatg gtattcccca ggcatgaggt taagatcggt
    63301 aaacagggtt tcaaccgaaa tggaactgct gtctttaagg tcataccagg tccctgtccg
    63361 cgggaatcga acactggcgg tgacgggttg gatatcaaaa ttaccggcca cgaccaggct
    63421 gatggagtcg ctttgaaggc tcagatattt cattggactg tttagccggt attgcaggct
    63481 ggcctggctg aattttcctt tgaaccatgg aaaattccgg atctcgatca gccttttata
    63541 ccagtcatac aactctttcc ttttggcatt ttgaagcaag ggccaaccgg gtggtttatt
    63601 gtccagccgg caattgttgt cgatcattcc gttctggcag gtgttgatgc tgaagtcata
    63661 agccagttca ccgaactgcc agatcagttt gggacccggg gtgagcagga aaaatgcggc
    63721 ggccatttta ttacgttcca ccgccgtatt aaaatcccgg atattatatg tgccgctgct
    63781 gagaccggat tgcaggttga ggtacatgag gcgttcttcg tcatggcttt ccatatagga
    63841 caccaggtgg ggctggctcc aaccccgggt ctggtacacc gaccagtcga aggtggatgc
    63901 ctcatggatc ccccgggtag ccgctccgta ggcgtaattg gcatttcccc aaagcagcat
    63961 gccataattg gcgagcgccg actcttcgcc gttgtccgca aaatgttcca ggatggtata
    64021 ggcgccgggg gaatactgct gaatgctgtc gtgataaagg cgccagatgg ccacccggct
    64081 cggatcatag tcactccagc gtcccacatc atttggattt tttacctggg taaacccttt
    64141 ggagaggtca aaccggtacc cgtcgatgtt gaagtttttc agccagtgct ccatcacgcg
    64201 caggctgaaa taccgggtat ccggactttg gtgattaaaa tcgttgaata cattaaaagg
    64261 gtgggtagcc gtgacattga accagggatt gtccgaagag ggccgttggt tgatcccatc
    64321 ccagtacaaa gccgccaggg ggcaggcgcc ggtggcatgg ttgagcacaa gatccaggat
    64381 caccgcgatg ccccgtccat ggcaggaatc aataaattgc cgcaggtcat tttcaggccc
    64441 ataatattta tccggagcaa agaaaaagga agggttgtat ccccaactct ggtttccgtc
    64501 aaattcctgt accggcatga gttcgatcgc attgaccccc agcctgctca ggtaattcag
    64561 ggtgtcggtc agcgttttcc agtcatgttt tgccaggaaa tcacggagta aaagttcata
    64621 gatgatcaga ttggtttttt caggacggtt aaacgtattt tgccaggcat agaccggtct
    64681 ccgggtttcc accactccca caatcccggt cgtttgacct gttgggtaag ccttgagtcc
    64741 gggataggtt tccgctgaaa ggaagggatc atggacggga tcgagtattt tttcagtata
    64801 agggtcggta atttttaaac tgccatccac caggtactgg aaggcatatt ccacaccggg
    64861 actcaggccg gtgatcctga gccaccaggt ccttccgtca tccgctttgt tcatctggaa
    64921 agcttgtttt tcggaccagt tgctgcctgg aaaatctcca ataacggcca ccctgttttt
    64981 aaaaggcgcc accaggacca ggatcaggga ggtgtccccc tcttcgtaat tgatgccggg
    65041 ttttaccccc tccggcaaag gttttttcac tatggagtcc gctaaaaaaa aggcaaagga
    65101 atccctggcg gcctggttcg ctgaatgagc agcaacagta accgtataac ttccgccggc
    65161 cggaatatcc agattcacag ccagggtatc cctggccggg tattgtccga ccttttgctg
    65221 atttaaaaac aattcccatt ccgcttcagc attactgacc gcttcaatag ggagggattg
    65281 gccagggaaa acctcaatga tttccgggat tggcttgtac aaaggctgaa aggcgggctt
    65341 gatgaaacgg gtggccagtt tatcatcgta aacagggata tacatatcgc tcccgtcgcg
    65401 gtttcgctga gccgcgcagt tggagcagcc tccggcccgg aaaaggatgg ctatatgcgt
    65461 gattttttcg gatgaaggga ccttgaaaaa acttctgatg ccggggatcg aatattgcca
    65521 ccggttttta cccaggttga cggcctggga tccagccatc tccgaccccc aggtaaaggg
    65581 aacgtatttc caatcggaag aagaagagct ggccgaagtg atcaccccga tatgaacata
    65641 gaccttgccg ttgtagtcca gcaatccttt gttccccttg gtggcatcca tgactatccg
    65701 cagagtatct tcagccttgg gaaaggccgg agcccactcc agcaattggg ccaggaccag
    65761 ggaatcgggc aggcaaaaca aggacagaag tccgattaaa aattgcttaa acggcatgta
    65821 ataaaggaat ttcgtatttt aattctgaaa ttaacttgtt ccggacttcg ttatacttta
    65881 agcaccaaaa ttaaggaaaa tgaaaagact gaatttagca ttaacgctgg ccattcttgg
    65941 tctgatcttc taccagtgtt cacgaaatcc tgttaccggg aaaaaacagt tgaacctcat
    66001 gtccgaagaa caggaaaagg cgatgggggc cgaaagcaat ccggcggttc tggctgaatt
    66061 tggcaaatat gaagatgcca acctgcaggc ttttatcaat gaaaaaggcc agcaaatggc
    66121 ggccatcagc caccgtcctc agcatggcta tacctttcag gtgatcagtt ctccggtagt
    66181 caatgctttt gcagtgcccg ggggatatgt ttattttacc cggggcatca tggcccattt
    66241 caacaacgaa gcggaattcg cgggtgtgct gggccatgag atcggccata tcacggcgcg
    66301 gcatgccaat gcccaggcca aggatcagct cctgacccag atcggggtga tcggactgat
    66361 cattgcctct ccggcggcgg cacagatggc cgaaaccctg atgcagggcg cccagctttt
    66421 attgctcaaa ttcagcaggg ctcatgagac ccagtctgat gaactgggcg tggagtattc
    66481 cactaaaatc ggttacgatg cccatcaaat ggcggagttt ttcaaaactt tggcccggct
    66541 ttccgatcag gctggtgcaa ggatccccag ttttctaagt acccatcccg atcccggtga
    66601 ccgctacaac cgagtacata aactcgcgga ggctgcccag gcttcgatca ataaatcgac
    66661 cttaaaagtg aaccgggacg aatacctccg caggatccac ggcctgatgt acggtgagga
    66721 tcccaacgaa ggctatgtgg aaggaaatgt tttttatcat ccccagttga gattccgttt
    66781 tccgataccc acgggctggc aaacccagaa ctcaccggtc cagtttgtaa tggccgaacc
    66841 caatggaaaa gccatgatgc tgctggtcct gggacagggt gcgaatttcg atgaggtcat
    66901 tgccaattcg aacaaacaat atggcctggc gccgatccgt tccgaaaaca ggaaagtaaa
    66961 cgggtacaat acggtcgtcg tattagccac ccagcaggtc gcccagggcc agcaagcgcc
    67021 cccggaggct gaaaaaccga tgatcctcag ttattatatc cagaaggacc agtatatcta
    67081 ttgcctgcac ggcgtctgcg ccaataaaga tttccagggt tatcagaacc ttttcctaaa
    67141 taccatggaa ggattcagca acctcaccga ggcttccaaa atcaatgtgc aggcggaata
    67201 catttatacg gtcaatgcgc ccagccagtc cacgctctca gcccttctta cccgctatgg
    67261 aatagaacaa aaaaggctga atgaatttgc cattctgaac gggatggaac tgacggaaac
    67321 ggttcctgca ggcaaattga tcaagatcgc aaaaaagggc aaggcctgaa gatttgatat
    67381 tatgatgagt ctggccgatg tcctgggtgc tttgggggtt tttctgatcc ttctggctta
    67441 tttttttaat ctgtacaaaa aaatagaaac cggcagcctg ctctatatcc tcatgaacct
    67501 ggccggggcg atccttgctt ttctgtcctc ggtaatgatc cactccctgc cctttatggt
    67561 attggaggga acctgggcgg tggtatcgct tgtggctttg atcaggctgt atttaaagcc
    67621 atggaggaaa acctaggata aaccatgccc aaagtgagca ttcctatcga cctgagtcat
    67681 cgatcaaacc tggtaaccta tgccaccttc aacccataaa ataaaatccc gcgttgagga
    67741 ggagcaggag agcctgaaaa acctgcgcac catacccgga gtaggcaagt ccattgcacg
    67801 ggacctgatg gatatcggca tccggtcggt gggaaacctg aagggtaagg atccccgaaa
    67861 gctctaagat cagtcaaacc ggcatgcggg aaagatacag gacccttgtt tgctttatgt
    67921 attccggtgc gtggtctatt tcgcggagac gccgccggaa gaacgggagg aagaaaaact
    67981 gaaatggtgg tggtggaagg agcaagactg atgctggtct tctatactca accctttagt
    68041 gtcccttgat ccggcccggg atgccggcaa tcccttccat ccaccctttc cctgccgtcg
    68101 tctgaaatcc aaaccggatt aagcggacaa tccgtatttt cccaatggaa ttccccgcaa
    68161 acgggagaga tgtcatgtat acggaccaag aaccataagc catgctctac ctttcaggaa
    68221 tcattctttc ctttttcctt tgttttttat tatttacaaa aaggaataaa acggcggcgg
    68281 atttcatcct gaccgcctgg ctgggcatta tcgggatcca tttgctctcc ttttatgtgt
    68341 tgtttaccgg tcagtttgtg aaatatccta ctttcgtggc gcttggtttt tcgttgcccc
    68401 tgacccacgg ccccttttta tatttataca cccggcagca aacgtcctcc gaccctttcc
    68461 aggcgaaaga actgtttcat tttttacccc tgtttttatc ctacctgctg ttcgccgggt
    68521 ttttcctgat gcccctggag caaaaaattg aagtgttccg gcaggccgga aagggatatg
    68581 aaacccagtc caggatcaat attttcgcga tcaatatttc cgggatcgtg tattcaggcc
    68641 tttccatcgc ccgtttgctg aaatacagaa aaaacctggt tcaccagttt tccaatacgg
    68701 acaaaatcaa ttttaactgg ctgctttacc tgattatctg gatcgtgttg atctggatca
    68761 ccatcatctt cgtacagaaa gaccagtgga ttttcgggat ggccgccctc ttcgttcttt
    68821 ggctgggata ttttgggatc aagcaggttc gtgtttttag ccaaaacgca cccctgctta
    68881 aaacggaaac ttcttcaacc gtccagagga atatggaaga taaaaggctt tacggtcagg
    68941 aaatacaaag ggaaacggaa gcattggcct ctgagaaaga atcggaaaac ctcaaatatc
    69001 aaaaatccac cttgagtgag cgggacgcaa gtgagattca tgcgcgcctg gaacgcctga
    69061 tggctgaaca aaaaccattt aaaaatccgg acctgaccct gaatgaactt gccaatagcc
    69121 tggacgttca ccccaaccat ctgtcgcagg tcatcaattc cagggttaaa aaaacctttt
    69181 acgaactgat caacgaccgt cgggtagaag aatttatcct cctgtccggc cagccggaaa
    69241 gtcagcagtt caccctcctt tcccttgccc atgattgcgg gttcaactcc aaggcttcgt
    69301 tcaaccggaa ttttaaaaaa tacaccgggc tgagtccccg ggaatacctg aaaaggcagc
    69361 tgaccgaagc ctgagcgggt ctcaaggctc atgaaaaggt ctcatcgctc ataatttatt
    69421 gtttttctgt tttatataag ctggcaagcc tgaggtctca accttcaggg tgaagcgttt
    69481 ggagggacct tgaacttcat ttttgtggtt caatttaaac ttcaaaaaac atgaaaaaca
    69541 catccagtct tctggccctg gcattcctgg ccctggtcag cttctcctgc tccaaagagc
    69601 gttttttaaa tgatcctggc aacctggtac ccaaaacggt ggaccaggac cccgggcttc
    69661 cttccatcct ggtaaatggc gtccttcttc attccgaggc ctttggccat ccggacagca
    69721 ccatgattgt atgcattcac ggcgggcccg gcggggatta ccgttacctg ttgaactgca
    69781 aagacctggc aaattatggt taccgcgtcg tgttttatga ccaaaggggg tcggggcttt
    69841 cccaaagact tcccaaagcg tcctacactt ctttgggatt ggctgccctg gacgccgttt
    69901 ttaatgaatt gggcggggtg attgcgcact acagaacaaa cccaaaccaa aaagtaatcc
    69961 tgttggggca ttcctggggc ggcattctgg ctacagggta tgccggcaaa aatcccggtg
    70021 ccatccaggg tttgatcgtt ggtgaacccg ggggcttgaa atgggcggag atctcggaat
    70081 atattaaaaa atcgaacgct tttaatttat ggagtgaatc gttaaatgat gccacgtata
    70141 cggatcagtt tgtcaccggc agggaagacc ggcatgaaat cctggattat aaactggccc
    70201 tcctggcggg caccaatgag atcaccggag aaaacaatac cgagccgggc agcgcctgga
    70261 ggtcgggtgc ggtgatccgg gacgcttttc tggagatcgg cgaaaattac aagcccgatt
    70321 tctccgaagg cattggccag tttaaaacgc ccgtattgtt tttcttcagt gagaaaaaca
    70381 aagcctatcc ggactcctgg gcccataaaa tatcgggagc ctatcctctg gcggaccttc
    70441 ataaagtcgc cggagtcgga cattcgggca ttttccagga taaaaaaaca tggcaggaaa
    70501 aaactttgcc caaattgctc acttatctga actcccttta aatccatttt acttaaaccc
    70561 aaataaaaaa atcattgatg aaaacttata ttaaatccat actccttagc ctgctgaccc
    70621 cgttcgtcag caccctggat gcccaaaccc tgaactgggc aaatctgcaa gaggaaaacc
    70681 ggcatatcat ccatgctcaa tttggcatgg aatacggtgc cgtgtttgga ataggttatg
    70741 gttatcagat gaataccggc ttgtttccca tggtggcaag catggaatac tccttcccct
    70801 ccggcaaaaa tttattggac gatctgaaaa gcaaaatcgg ggtacagatc cgtttggtac
    70861 agtaccgtca tttccggttc agcgccaaac tgcagggtgt tttccgcagg cacgagaccg
    70921 gcctggtgcg cctgcttaat tttggaagtg atttttccgg tatcctcggg atctatcgcc
    70981 ccaaatggtt tttcgccggt gaatttggtt tcgataaagc tattgtaagc cacttcaaac
    71041 attcaaaggc ctacagggac cagcatcccc aggtcgtcga cgggtggtat gaaccggcaa
    71101 ccggaggaaa tttttattac ggtgtgcaaa caggattttc cattaaaaac caggatattt
    71161 ttctaaaggg cggaaaaatg tttgctcagg attttaaaac aaagcctctt ctgccctttt
    71221 atggccagct aggggttaac ctcaaattct gattcctgac catttttata aatgttcaac
    71281 ccaaaccatg caatagaatt tctattacaa accggaatgc ctgcaaatac tg
//
LOCUS       DAOQXT010000012        63370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_494_length_63370_cov_6.967148, whole genome shotgun sequence.
ACCESSION   DAOQXT010000012 DAOQXT010000000
VERSION     DAOQXT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 63370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63370
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_494_length_63370_cov_6.967148"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1422)
                     /gene="manA"
                     /locus_tag="PKM27_03290"
     CDS             complement(232..1422)
                     /gene="manA"
                     /locus_tag="PKM27_03290"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HNR06313.1"
                     /translation="MSSFERLEPYLQHYAWGGFSYLPQWLGIENPGHQLFAEAWFGAH
                     PNHPSLILSGHERSSLKERIEADPLKELGAESVSTYGPRLPFLLKILDVRDMLSIQLH
                     PDKKGAEVGFEEENNRGIALNDPKRNYRDDNHKPEMLLALSDFWMLQGFAPLEHIHQR
                     MSLYLPELEGRTGGLDWKGLLELFLSLDQEMVNHSCSQLASRLSPGQTANKKNIGFWL
                     ARAMETYPAVKGQYDRGLLVMLLLHIQYLRPGQAGYQAPGVLHAYLEGQNVELMSNSD
                     NVLRGGLTPKHIDIREFLKHADTSRAGQHILKPDYLMDDELVFTPPVPDFLLKHFKLN
                     PGDSKTIQFNTASIVLIHSGKVETPEGQVFGKGQAVWLPCFNVLKIKAMDDQAELFLA
                     TATV"
     gene            complement(1422..3074)
                     /gene="pgi"
                     /locus_tag="PKM27_03295"
     CDS             complement(1422..3074)
                     /gene="pgi"
                     /locus_tag="PKM27_03295"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNR06314.1"
                     /translation="MIAAINPTKTQAWEKLKTHFRETRDLRMKDQFAWDPDRFEKFSI
                     RWGDFLVDFSKNRINETTLALLLELAEECKLGEGIESMFTGEKINTTEGRAVLHTALR
                     NISGSPVWVDGENVMPEVYKVLGQMQAFCEKLHRGQWTGYDGSTISDIVNIGIGGSDL
                     GAVMVTEALKPYWDRGITPHFVSNVDGSHIYQVFQKIKPATTLFIISSKTFTTQETMT
                     NAHSARQWFVHQAGEVHIAKHFVAVSTNAAEVTGFGISAENMFPFWDWVGGRYSLTSA
                     IGLPIACTIGFERFRELLEGFHAMDLHFRNTPFERNIPVLLALIGIWYNNFYQAESEA
                     ILPYDQYLHRFPAYFQQGNMESNGKGVDRNGQIIDYQTGGIIWGEPGTNGQHAFYQLI
                     HQGTKMIPCDFIAMAQSHNPLSDHHPKLMANFFAQTEALLNGKSLEEIKEEMKKAGLS
                     PAEMERLAPFRVFEGNRPTNSILIKKLNPQTLGSLIALYEHKIFTQGWIWNIYSFDQF
                     GVELGKKLATQILGELASEQPTEKHDASTRGLINAYKQMRHS"
     gene            3121..3486
                     /locus_tag="PKM27_03300"
     CDS             3121..3486
                     /locus_tag="PKM27_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNR06315.1"
                     /translation="MSRNQKTGKEGEDLAARHLEGAGYEILERNWRFSRAELDIVCRK
                     EGILIFVEVKTRSYTFFGEPAEFVRSKKERLIMDAANAYMASIGHEWQFRFDIISIVL
                     SPDGRHRLEHVEDAFFPGW"
     gene            3587..3835
                     /locus_tag="PKM27_03305"
     CDS             3587..3835
                     /locus_tag="PKM27_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNR06316.1"
                     /translation="MKKDIHPSNYRMVVFKDFSADEAFLCRSCTPTKDTIVWQDGKEY
                     PLIKLEISNKSHPFFTGKMKFVDTAGRIDKFNKKFKKA"
     gene            complement(3938..4366)
                     /locus_tag="PKM27_03310"
     CDS             complement(3938..4366)
                     /locus_tag="PKM27_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR06317.1"
                     /translation="MVKVELLTGIDQVGADRLNELMAQLCPECPAHPLTHFRDILYHP
                     TTRLLVAMKEGEIRGCLTLVWYKIPSGIRFWIEDLVVDSPYRGQGIAKALIQHAMEQV
                     RALGGHTIDLTSRPSRIEANQLYRSLGFEKRKTNVYRISL"
     gene            4486..4559
                     /locus_tag="PKM27_03315"
     tRNA            4486..4559
                     /locus_tag="PKM27_03315"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4520..4522,aa:Ile,seq:gat)
     assembly_gap    4677..4776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4814..4887
                     /locus_tag="PKM27_03320"
     tRNA            4814..4887
                     /locus_tag="PKM27_03320"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4848..4850,aa:Ala,seq:tgc)
     gene            5721..7088
                     /locus_tag="PKM27_03325"
     CDS             5721..7088
                     /locus_tag="PKM27_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06318.1"
                     /translation="MRKISAYVFTPVVGYISYSIAVILNSILAEDWSSSIPGLGLFIF
                     GNMAAIVGMAKFTSWLTPSDNLPYIVSVLLYICYTIWGMYLNPQFTILYYLIILLTGF
                     GALVYSQHEMKTKNISDEDDSCENTISVDSNSQTKLPWPKYESGPKDKRQQNDDSDNN
                     IKIVLLLSMLLVGFFIYQFLNEKDNNNHNKPSAEKSKPNEIPNNPNQINNLNANLNVL
                     ADNRPNRTELNASMNESSDPNEFILAFLRYINAKNFDKAYLMTLNPKWEPKDSFSTQG
                     IWGGFRALEDIVIDSDVGYSSGFGDKVLRVKYTAYGVNAAYPQKYEWDYHLKKIDYKW
                     QIIKMTLPKITDYKLGNINSPLDAVNLFFRSLEQGDFSVAFALTRNPEWKKFSIFIQS
                     WGTISDLKVYEIKEDIGTLSPSFNVKYYSNNAKTGLSGIFIYNFQLEDINNVWRITKV
                     IKLYN"
     gene            7229..7741
                     /locus_tag="PKM27_03330"
     CDS             7229..7741
                     /locus_tag="PKM27_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR06319.1"
                     /translation="MRYYLKTHLLILVYLVLHSSLQSQTYKELFYAGSIKYQKGDYKG
                     AIIDYSKAILLNSNNFEPFLLRGLAKIELGDFRGAILDYNQAIQINPNYDEAYINRSY
                     AKAKLNDFTGQILDLNFAIKLKPSPENYIERAKAYIKTGNKENGCLDFSKAGELGHPN
                     AYEYIKLYCN"
     gene            complement(7834..9087)
                     /locus_tag="PKM27_03335"
     CDS             complement(7834..9087)
                     /locus_tag="PKM27_03335"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNR06320.1"
                     /translation="MKLLIFIITCLTTTIVNSQTPLNKLNLILEFYNNGDYHSISEIL
                     KDNGFKLNTVIPRIKNGSENILENLTYIKKTEVKLICRICPNEYEPKIANESFSIVKD
                     INLANLGPIPTSPYNDDKFVKMVNIMKTDEDFLRLYRNTVGEYMSIEDYIWSYLEPRY
                     GSLPSTPLEVIKISYEEYIPFGSAMDILSLYSRTIKILSPYKILSNPTFYIEYTKDDK
                     DLGWGKYYYYYKNNNKSDKLISDYKYILQEIDIEFLGVTDLNSSNSGHDIKLKTNIKV
                     RNNRYYEDKIGSSEQVPIYHGPGGIYQVVIELNGSLKTRITFDTGAGDVILSPEIADL
                     LRKSGALSASDYVGKSTYSYADGSIESLQQYLLKSIRIGNKTIQNVICAIGNKPNSSS
                     LLGQSFLKRFGAYEFDYKRNLLIFK"
     gene            complement(9142..11472)
                     /locus_tag="PKM27_03340"
     CDS             complement(9142..11472)
                     /locus_tag="PKM27_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR06321.1"
                     /translation="MNEAETRAELIDPKLKGCGWGVVEGSKILREYYITAGRIQTGGI
                     RAKRLKVDYVLVYKGLKLAIVEAKSDELEVGEAVAQAKQYALKMDLETTYATNGKSIY
                     SISLKTGVEGLVDNYLSPEELWNKTFANQNLWREKFASIPFEDRSGTWHLRYYQEIAI
                     NNTLEAIANKQNRILLTLATGTGKTAIAFQIAWKLFHARWNLKYDGSRRPRILFLADR
                     NILADQAYNAFSAFPEDALVRIKPDAIRKRGAVPTNGSIFFTIFQTFMSGTDSEGKSA
                     PYFGDYPPDYFDFIVIDECHRGGANDEGNWRAIMEYFSPAVQLGLTATPKRLDNADTY
                     KYFGDPVFIYSLKEGINDGYLTPFKVKRIKTTLDDYIYTSDDQIVEGEIEQDKLYTEP
                     DFNKKIEILEREAKRVKIYLDEVNQSEKTLIFCATQEHAAIIRDLVNQYKKSKDPNYC
                     VRVTANDGEIGDQFLREFQDNEKTIPTVLTTSQKLSTGVDARNIRNIVLLRPVNSMIE
                     FKQIVGRGTRLFDGKEFFTIYDFVDAYHHFADPEWDGEPVEPDVPKRQGIGEENPPKD
                     LHEPEEEEVKRELPQKIKIKLKDGKEREIQHMISTSFLSSDGKPISVEEFLQNLFGSL
                     PEFFKSEEELRTLWSNPLTRRTLLEKLDEAGFGKEELTTLQKLIDAEKSDLFDVLEYV
                     FNSDFKPITREARVLAAQATIFALLNDRQKEFIEFVLSKYVETGVEELDQDKLPVLLT
                     SKYQSLPDAGGKLGDLTKVKDLFIGFQKFLYERRVG"
     gene            complement(11585..13030)
                     /locus_tag="PKM27_03345"
     CDS             complement(11585..13030)
                     /locus_tag="PKM27_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR06322.1"
                     /translation="MRDQELKDLLNDLTKASTESEWVEFKLNFHSIEEIGMCISALSN
                     SACIQNQPLGYLVFGVEDKTHNIKGTSFKAKEYKKGNEDCEHWLATRLNPRIDFLVFE
                     FDYDVNRHISIYIIPATKNRPVEFLHQAYIRINTIIRKLNEFPEKQAKIWKKDFPPFE
                     KEIARDNLVASDIIKYLSTETYFDLMKLPYPSIQQAVIDKLIEEGIVVKNKGYAITKL
                     GALLFAKQLKDFESVERKSVRVIVYKGKNKVETEREQMGVKGYALGFVGLVDWINSQL
                     PANEEIGKALRKDTRMYPEIAIRELVANALIHQDLAEKGFPMIEIFSDRIEISNPGTP
                     LVKPERFIDAYQSRNESLADLMRRMGFCEEKGSGLDKVIFYNELFQLPPINVTASENR
                     TRVTMYCYKSLNDLDKKEKISACYQHACLKYVSNEKMTNQSLRQRFKIEDHNYSIASR
                     IIRDAVVDGVIKEDDPESKSRKYASYIPFWA"
     gene            complement(13049..14185)
                     /locus_tag="PKM27_03350"
     CDS             complement(13049..14185)
                     /locus_tag="PKM27_03350"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNR06323.1"
                     /translation="MKQNWQIKKVGEVCEVIAGQSPEGKYYNNELKGLPFYQGKKEFG
                     EKYIGEPTTWTTQITKEAEANDILMSVRAPVGPINFSTQKICIGRGLAAIRASKLIDK
                     EFLFYFLLKHENEIVGNAGAVFDSINKSQIEAIEIPVPPLSEQKRIASILDSAFATID
                     RAKANSEQNLQNARELFESYLQSVFENNGEGWEEKTLGEVIDLVSGQHIDSKEYNFKG
                     KGIGYLTGPSDFGVIHPVVSKWTEFPKQSAIKNDILITVKGSGVGKINLMSESELCIS
                     RQLMAIRAKTVNVSFLYAFLSTKYYYFQSLSNGAAIPGITRVNVQSLKIKIPPIRHQQ
                     VIVQKINAFSSETKKLEDIYQAKINDLEELKRSLLKKAFSGELN"
     gene            complement(14182..15648)
                     /locus_tag="PKM27_03355"
     CDS             complement(14182..15648)
                     /locus_tag="PKM27_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNR06324.1"
                     /translation="MFEQAFKNIDDILHKDAGCSSELDYVEQTSWVLFLKYLDDLEKD
                     KATAAQLTGKPYKNIVSPEYQWSKWAMPKDKTGKLNHHRTLTGDDLKDFVDHKLFPYF
                     KRFKTSADSADTIEYKIGEIFSELRNKIQSGYNLREVINRIDELRFRTHVEKHEMSHL
                     YEDKIKNMGNAGRNGGEYYTPRPLIKTIVKVVAPKIGDKIYDGAVGSAGFLCESYEYL
                     RHSKGKPENLTSNEVETLQKKTFYGKEKKSLAYIIGIMNMILHGIEAPNIIHTNTLAE
                     NISDIQEKDRFDIVLANPPFGGKERAEVQQNFPIKSSETAYLFLQHFIKILKAGGKAG
                     VVIKNTFLSNTDNASVSLRKLLLESCNLHTILDLPGGTFTGAGVKTVVLFFEKGASTR
                     KVWYYQLNLDRNLGKTNPLSENDLSEFIKLQKSLSNSENSWTIDIKAIDKFTYDLSVK
                     NPNKKEKTGLRKPHEILEEMKSLDSESADILNSILMIL"
     gene            complement(15931..17541)
                     /locus_tag="PKM27_03360"
     CDS             complement(15931..17541)
                     /locus_tag="PKM27_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06325.1"
                     /translation="MSSSKKMTVIVPVPKRICNIITTVFGNTTKGVKALSIIKEMIFQ
                     HQKCFAPMLHYLPYDYKTKKKLLGGSEFYPIWNELEKLEILEPKVLPGNRAYSYSGHI
                     CKSYRISPKWLDGDVMYLEHARNLNDFKVDDENMCRLIENTLKYVNVSTSDGQINNKS
                     DSLSQDYIDNKFKITMKGIKYKYAKISYKESLSSEYIKPYLSFEKINISESYKGGLKL
                     LNLKHFYISKSTTNGRINYNLTNLPSELLKLLDLDNNCLCEIDLSCSQPLILSYLIKS
                     IINNNMEPISADRFLNQSIKKHLSPPLLFSSNIPPSDNTSKSDIEVFMEKTEEGQLYE
                     YFSTQLFGSCNETTRKEAKLSFISSLYSKSESRNVGKLKFAKVFPKLTKLLDSVKIDM
                     IETFEDHLKNGSFPELNKYTFSNRKGNKDAKSAGNDFLAVKLQEIESTIFLDHILPDL
                     YRSGLMVLPKHDAILCRQEDLSIVMEIVTTHLDRIFGAGNYKSRTKVLNEVPNSCSNL
                     SSATIKNERTKESSDMDSQEKNLLKQFS"
     gene            complement(18111..19358)
                     /locus_tag="PKM27_03365"
     CDS             complement(18111..19358)
                     /locus_tag="PKM27_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNR06326.1"
                     /translation="MNRPKFNLKNKSEKETLISMIFRPLKQRLVYSTGLKIPPKYWND
                     NTHRAKATQDFIDYQFINHELDKLESLALSIAKQYISQSKILTSLAFKGEMDALYHGK
                     IRHSGVKDFSSFMDKFIAERSRSPKYKPKSIAVYQTTKNHFNRYAKGKVVYFEDLSIE
                     FLLGFIQHLYDKDYSNNHINKIITTIRTMLNDATDRGFNKNMDYKSRKITVSKTETDN
                     IYFTESDLKKLILLDLSGNPRLDKIRDLFIVGSYTGLRYSDYSILQPQNLRFLDGVKV
                     FDTTVTKTGDRLIIPLHPIVEPILLKYSNKLPRPISNQKMNDYLKELCKMAELDDIII
                     KREYRGGKMYEASYKKYEMVASHTARRSFATNAYKAGVPMLSIMRITGHKTPEIFLKY
                     IKFDNEENALLMARHSFFNAISN"
     gene            19845..20222
                     /locus_tag="PKM27_03370"
     CDS             19845..20222
                     /locus_tag="PKM27_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06327.1"
                     /translation="MNQKNVFTALAILLMVQALAFYFMGGKIAMDAFGDLGEPGNMAA
                     KHLMEIMAAFAFNFGLLAYATRSNSQVLWVFTLGFAIVVVVTLKHILADGIHVPIWAI
                     AFQILSLLAVGYLWMRHNKVKPA"
     gene            20382..20987
                     /locus_tag="PKM27_03375"
     CDS             20382..20987
                     /locus_tag="PKM27_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06328.1"
                     /translation="MFPFQFLLILLLTGAERSDATNDQFLLFDVEFTYTKEEADHSKP
                     SKSHYYVTGDMLNTQRPADWTSPVDYRNGSVHIRLEVIEKPQGGAPTTWTLCYIPNQG
                     QGNGYGCTGTGLYTDKGIYEKDIAMTSFWENESIIWSKGIKQMDLVIKDDSGGQGHAH
                     KRQDHERFFPTRVRITMVQVAAGSTYDPGILPKTALYREDR"
     gene            21199..22002
                     /locus_tag="PKM27_03380"
     CDS             21199..22002
                     /locus_tag="PKM27_03380"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNR06329.1"
                     /translation="MYKASRLLFRLFLGLFVLYLIGFGIIYSMQDRFIFQRQVLDPNH
                     RFGHAAQELYIPAGQDIRLNALLFKPEGTPKGLVLYFHGNRHSLDRWGRFVQFLINFG
                     YEVLIFDYRGYGKSNGEPSEEGLYEDAEAVYDYARTHFPEKPLIYYGRSLGTGVATHL
                     AAKHAPQKLVLETPFDELKNVVPAYFRFMLHIIPLRHAFRNNERLASITCPILILHGT
                     QDPVVPLSSALGLKPLLKPGDQFRIVPGGRHNNLTRLDEGIDALKSFLN"
     gene            22044..22973
                     /locus_tag="PKM27_03385"
     CDS             22044..22973
                     /locus_tag="PKM27_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HNR06330.1"
                     /translation="MKNKIFFALGLLLSAALLQAQHLGAPPEYVKALTPLWKGERFAD
                     GRPKVPDIVLESLESATLEQIWGFLGRKGFRNQVEKDWIILKPGETMVGRVVTAQFMP
                     SRPDLDTLVRSLGKAEGRSQKGGINIWPIDILTKGDIYVADGFGKIKDGTLIGSSLGN
                     AIVGKTGKGVIFYGSVRDMQELKDTKGFNAWVKGHDPSFIRDMTPTSINAPIRIGQVT
                     VFPGDVVFANEYGVVFIPAYLVEELVSSSTLVGLRDEFERLLLQQGKYPSGQIHGDWS
                     EEIKNEFRAWFAKYPKQLPVTKEDIEAYLKRGE"
     gene            23032..23961
                     /locus_tag="PKM27_03390"
     CDS             23032..23961
                     /locus_tag="PKM27_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HNR06331.1"
                     /translation="MKFSLTILFCSFCLLTWNATEAQTISREELIFLTSEWQGERFAD
                     GRPKIPDDLLDRARRINIDDAWTVLKNYGYVNQFEGGWKMVKEDLPVIGRAMTAMFMP
                     SRPDVERKIKERGTSRDGRKGNTNSWPIETLVKGDVYVADGFGKIGGGTLMGSTLATS
                     IYSKSGNGVVFNGASRDLQEISNMENFNAFVRDFHPSFLEEMVLMGLNTPVRIGGVMV
                     LPGDLVIARRTGVLFVPAHMAEEVVSTAEFVIRKDQFGYEMVRTGKYSTGQIDSQWTE
                     ELKAEFLKWLEKRPDLGKMTKAELDQRMSKRTW"
     gene            24155..25693
                     /locus_tag="PKM27_03395"
     CDS             24155..25693
                     /locus_tag="PKM27_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HNR06332.1"
                     /translation="MKKAIHQLIQSNKNQEAERAKAITGEINNPATLPARRDFLKKTA
                     LGGIALTGLGHLSAAETIEKASSKVSRMSAPTGLKITDMRYTTISNGTGATNARNVIL
                     RIDTNQGIYGLGEVRDGGDARFALYLKSRILGLNPCNVEMIFKIIKQFGGHGRMGGGV
                     SGVEMALWDLAGKAYGVPVWALLGGRYRDKIRLYSYIPNSGGRPLAEMDLEQFKADVK
                     RRMEVQGFTWLKMHPGIEKVAKIPGTTVNTKFIPGFNDRVSSSRSQSYLHYQNTRHAF
                     TSVQLTDKGLEELARYVETIRNAVGYEVPLSADHQGHFDINTCIRVGHALERFNLASL
                     EDFLPWDQTEQLKKITDTINTPTMTGEDIYLKEAFNNLCDHHAVDIIQPDMGSSGGIL
                     ETKKIGDYAEEKDTPMVQHFAGTPVCFMANVHCAAATQNALALEVPNQIVDNPWWPNL
                     VKMVGKQPLYEKGYANVPDDAPGLGVELNEEEMKKHLHPEDPSYFVPTPQWNEVRSYD
                     RLWS"
     gene            25741..26712
                     /locus_tag="PKM27_03400"
     CDS             25741..26712
                     /locus_tag="PKM27_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06333.1"
                     /translation="MKSYLALFMVLLAVRVGAQGQFPSASISNGLIKAGFYLPDKDKG
                     YYRGSRFDWSGVMPSLEFQGHQYFGQWFERYSPTLHDAIMGPVEAFYPIDFETANPGE
                     DFLILGIGVLTRPDDKKYSIATTYPIKDPGKWKVRKKTDQVVFRHKLHGLGYAYDYQK
                     TVRLEKGKPELVLSHSLKNTGNRAIETNVYNHNFFVMDSQVTGPDFVVQFPFKITGEV
                     QRGNELGGIRENEIVFYKSIEKNKSLYFGALEGFGQDPRDYDIRIENHKTGAAVRITS
                     DQPLMKLAFWATQMTLCPEPFTRISVKPGEEFQWEVRYEFYTCAIRD"
     gene            27093..27545
                     /gene="tnpA"
                     /locus_tag="PKM27_03405"
     CDS             27093..27545
                     /gene="tnpA"
                     /locus_tag="PKM27_03405"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNR06334.1"
                     /translation="MGQSLVKIYTHIVFSTKHRQAFITDKIEQELFSYIGGICNRLGC
                     QTITVGGYRDHVHILCLLSKQITIMKLMEEVKGHSSKWIKTVGQEFRNFYWQNGYGAF
                     SVYSKDVDRVVQYIRSQREHHSGRSWQDEYRDILIEHEMDFNEKFVWD"
     gene            28074..30254
                     /locus_tag="PKM27_03410"
     CDS             28074..30254
                     /locus_tag="PKM27_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNR06335.1"
                     /translation="MKIIHYITLSGILWFAACKKPDSYANLLADGTGKDYPAYGGNKA
                     NTRYSSLDQITPDNVNQLKIAWSYFANNPPDTGRPRSGGEIQCQPIVVHGVLYGTSAE
                     LNLFALDAATGTELWKFTPPQENLRRHASRGVMHWESGEDQRIYYTAGYHLYCIDAGS
                     GKIINSFGDSGRVDLHAGLGENLDHEVNKLHVTATSPGIIFKNTLVIGSSVSEGGDAA
                     PGHIRAFDVNSGKLKWVFRTIPQPGDPGYETWPPDAWKKFGGVNNWSGMSLDEKRGLV
                     YFGTGSPSSDYYGGDREGANLYGNCVIALEAETGKLKWYFQAIRHDLWDRDFPCPPNL
                     ATITQNGKKREVVVQAGKDGYIYVLDRDTGESVFPLEEHPVPVEGLPGEHPYPSQITP
                     AKPRPLIRQLFTEADITDISPESHAFVKKRLQEFPPTTNRHQPPREAGTILFGYSGGA
                     EWGGGAVDPEGILYQNANEAPWELTMIDKASRDKETATLSPGHSLYLRNCAACHGADR
                     RGNSNLVPTLVDIGKKRSAEDIASFIQTGNARMPAFIYLSEKERKALAAYLLETEGSV
                     MPAPRTAEVMKEKNRPDFPYEPPYMIKVWQRFVDQNGYNAVNPPWGTLNAIDLNTGEY
                     LWRVPLGEFPELTQRGIPVTGTETYGGPVVTAGGLVFIASTRDERIRAFDKRTGKVLW
                     EYQLPAGGFATPITYEVSGKQYIALAVGGGRGAKPGGWYMAFSL"
     gene            30348..32375
                     /locus_tag="PKM27_03415"
     CDS             30348..32375
                     /locus_tag="PKM27_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNR06336.1"
                     /translation="MKGSLYGLVFLTILLAEVGILSCRNGGSGMESRLERSLAKAGSN
                     RPELEKVLEHYAFAPSDSLKYKAAIFLLANMEDLIHFRSKWQEQFDPVFFKAMAGKTV
                     EEVGPLRKSVDSLIGKPDWKSFEKIPDLRTLTSEYLIQNIDQAFEAWRSAPWSGDVSF
                     DAFCNYILPYKNHNEYPEVWRKTLRDRYGHLLTDSVVPKTMTDICCALVDEQRWFDWT
                     DDYGFYPSVFGFSQLMEGRKGSCTEMAHLGAYTARALGIPVAVDYIPQYGNMHGSHVW
                     NALITSDSSYLYFEGAGGTRPGDMFYTREKDFRLAKVFRRHFDLVQGSYAARAMEAGL
                     KDIPELLQNPRIIDVTGGYTQVANLRIHGLGREGEPVFLCIYKWRNWTAVAGDWVKDG
                     QASFPQMGRGLVYMPMYYRLGEYEPAGPPFVLPVHGNIWYPEADEKNRQSVLLKRVDL
                     FKRNWEYNLANQMTGARLEGSADSNFSQPVVLHQIQAPVKIYRGALVNDLDQKGRASY
                     DSTWKTVTLTRPEKFRYVRLIFDEPLLFRLGELEFYDSESGPPLTGIPAGNVPEPSRA
                     FDGFPGKTIKMTEETNRSHWVSLDLGSPKSIRKIRYLPAHDARTVQEGKSYELFYWKG
                     RWESLGSQKCNGLPLEFQEVPSNGLYWLHCRACDDKEERIFTYEDGKQIWW"
     gene            32541..35429
                     /locus_tag="PKM27_03420"
     CDS             32541..35429
                     /locus_tag="PKM27_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNR06337.1"
                     /translation="MSASTIIPPDLNIKQWSKNIKGEVLTDRYSLGMYATDASFYQIW
                     PVAVVLPRDEEDVKKAVAFAGQHKLKILPRGGGTSLAGQTVGDALVLDFSKYMNQVLE
                     FNEQEKWVRVQPGLVRDELNEYLAKYKLHFAPDPATASRANVGGMVGNNSSGTKSILY
                     GKTVDHVLEARVLLADGTVLKLRELDPEEYRRKTSQHCREGQIYRGFQEIIEKNREEI
                     KNRFPKVMRRVGGYNLDEFVYTDRWNLAKLITGSEGTLAVTLELKLNLVPLPKFKSVV
                     VAHFADLLESIRAVEPMLPYQPSAIEILDRTVLHLSAENLTTRHLCYLIEGDPAAIQI
                     VEFYGDTRESVMQRARDMIADLHQRGLGYAFPLFPEGPAFEDVWTLRKKGLGLMMGIK
                     SEKKALSFIEDAAIPTQVLPEYIDQVLKICAKYHTEVSMYAHASVGVIHVQPLLDLRQ
                     AEDIENLKHITDETFELVVKYGGSWSGEHGDGLVRSAYNERFFGRTLYEAFRSIKKLF
                     DPENRMNPGKIVDSTTIEHHLRYGTAYRDREVKTVYHYRQENSFRESVHMCTGVGECR
                     KMLGGTMCPSFKATRDEEHSTRGRANALRLAMSGQLDEEGIGSERLHQVMDLCLSCKA
                     CKSECPSNVDMAKLKGDVTQIYYDQHGTTWRDRLIRDSSSMAVRLAGWPARVVNGIQG
                     SALFRYVMDKCTGIDSRRILPGYATEPFYKWFEKNKNKVNPPAQKKVVLFADTYLNFH
                     EPHIGVSALELLNSCGYEVILANVGCCQRPKISHGFLRDAKAAGTATLEKLRPYMEQG
                     LKIIVCEPSCASALNDDLPDLVEDEKLAQLAKDHILMIDQFLSRELESGNINKKFKSI
                     AGDILIHGHCHQKALYGTRAMKEVFGQTGNSLSEIPSGCCGMAGSFGYEKEHYDLSRK
                     IGDQILFPAVNNRKPGTTLVANGFSCRHQIEHFTGVKAKHWVEVIRGV"
     gene            complement(35709..36278)
                     /locus_tag="PKM27_03425"
     CDS             complement(35709..36278)
                     /locus_tag="PKM27_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR06338.1"
                     /translation="MRYQSHQVYHIFNQGNNREKIFFEPCNYEYFKAKLRRYVCPNAD
                     LLAYCLMPNHFHLLIYTSDLSVQEYKNSGLQNISFAISQLLSSYTKGINRRFNRTGSL
                     FRSKTKKKDGWNNDNDFRSIAEFEGKALSHVENVYALRVFNYIHENPVKARLVRTAEE
                     WVYSSAREFSKVEEKPLCNIERAKYLLGV"
     gene            37282..39045
                     /locus_tag="PKM27_03430"
     CDS             37282..39045
                     /locus_tag="PKM27_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002191185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HNR06339.1"
                     /translation="MTWTQNTDPLNNIAWSALVAALPVILIFWLLIKKMKGYQASLLT
                     VLFSIVIAVVVYGMPLKLSLLSGLHGALYGLFPICWIILSAVFLFNLTIKAGQFEIIK
                     NFMASVTPDRRLQALLIAFSFGAFLEGAAGFGAPVAISAAMLVGLGFNPLYAAGICLI
                     ANTAPVAFGSVGTPIIMAGRVSEIPEMAISQMVGRTLPILSVVLPFYLVVLMSGFKKS
                     LQVFPAIVVSGLSFAFFQWFAANYLGPMLPDVMAGIASIFCLMILLKYWKPSTVWRFN
                     EEPEQTLDSSLKYSAGQVLRAWSPFIIMTIVIVAWGLQPVKDSLNHLGMLRFDIPGLN
                     GAILKNDGSPLVIRAFEFNYLSAPGTAVLFATLISIPLIGLTYRQGLEIFRITLKQLR
                     FPILTVAAVVGFAFIANNSAMSITMALALAGTGALFPFFSPILGWLGVFLTGSDTSSN
                     ALFCKLQSTTADTLGIDPVITVSANASGGVTGKMISPQSIAIGAAAVGLIGKESDLFR
                     FTLKHSFIMLLVICLLTTLQAYTWEGLIPSYTKPDGQTMAAVADGTHGFTYLMIMALA
                     VLLLVMTVYLTNRKSKERGKP"
     gene            39091..40971
                     /locus_tag="PKM27_03435"
     CDS             39091..40971
                     /locus_tag="PKM27_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06340.1"
                     /translation="MHAENLIIRDNLKDLYPDVYTPEALSRLSSMASFNRDIKEIMDL
                     RMQRRSERQEKQKRIGFLDPESLIPGTSIRVGDAREGRFEGSAIPGDLQRQWIQGTGP
                     AAKPKAPLESSIRNVAYALLSGADGWMFDGEDALGQINTMSLDNQRNLKLAIHRDPVF
                     LKVAEQVAKEMNRWSMDFFGRAIISDWEQQLKFTTIIFRARGLHLDDRHIREAGGLGL
                     AASIVDLVLYVVNNHEALAGAGSSIVLYLPKIQTAGEAALWNDMLTALENLLGLQHGT
                     IKVYVLVEQLEATYQLMEIRAALGQHFIGFNTGRWDYINSVADAMAWDPSFINPNIEA
                     ITMTYGYMRNYEDRVRRAVNTPDLRGGYALWQGGMEPNIPVGSAEGVSAGMKKAVAGA
                     QREQHDGASGKWVSHWKMVHIVRPVWEKAGALNQAGRAFPPLTYTQADADGLVLLEPA
                     PRSIRGARNLISVALQYGNAFLQGMQAAALKPADFFGDEDILYLMEDMATGEIRLSIL
                     WEWVHKGAVLTEDDEASGIRAGSRFDSDLFIRLLDEEYQKLLSASDKDVHDDSKTTTL
                     PIAREIAAAYVSEEIKAPWFIDLLNLNLNNQDLERAKERIGLYLDTFRKEGRRITENL
                     NF"
     gene            41118..41636
                     /locus_tag="PKM27_03440"
     CDS             41118..41636
                     /locus_tag="PKM27_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06341.1"
                     /translation="MRSYFILGLWMFAGICLAQKPAAPLTSNLNLSKSNINRLIYPEP
                     LLTAVNARLLLADLEKINPPDEAGLKTWLVANFKRFGIQPDQVKQIVIYSSRSYSDCT
                     ICKKNCKGHCVQDPGSDCVCIHKSEPNLRTIDQGKAGNVIYFSLSAVGEKEALDKILN
                     TIQAQKTAAVTN"
     gene            complement(41687..42637)
                     /locus_tag="PKM27_03445"
     CDS             complement(41687..42637)
                     /locus_tag="PKM27_03445"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HNR06342.1"
                     /translation="MPQKKIITIFGATGAQGGGLARAILSDPNSEFAVRAVTRDAHSE
                     KAKALAGLGAEVVQADLTDPESVRKAMEGAYGAFCVTFFWEHFSPEREAQDAKVMAEA
                     AKATGLKHVIWSTLEDTRDWIPVHDDRMPTLMGKYSVPHFDSKGESNELFTRLGVPTT
                     FMATTFYWENMIYFGMGPKRGPDGVLAITFPMADKRLSGIAAEDIGKCAYGIFKKGPS
                     MAGQTVAIAGEHITCADMAAAMTKAFGEEVRYNAVSPAVYRSFGFPGSEDLGNMFQFY
                     QEFEQMFTEKRDIAKTKELNPELQSFGQWLAANAKRIPLN"
     gene            complement(42739..44037)
                     /locus_tag="PKM27_03450"
     CDS             complement(42739..44037)
                     /locus_tag="PKM27_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR06343.1"
                     /translation="MRFVFILILAALSPNPVSSQDLYFPPLLGNAWQTLDPSTLGWCP
                     DRIDSLYGFLHREQTKSFLVLKDGRIVLEKYFGTYTRDSLWSWYSAGKSLKAVLVGIA
                     QEEGFLDLDDRVSKYLGQNWTSLPAEKEDKVTIRHQLTMTSGLNELFFFCVNPNCLVY
                     TADAGTRWVYHNGPYTLTKAVLEKASGQALNPYMTSKIKLKTGMGGLWIYTGTNSLYY
                     STARDMARFGLLILNRGRWGNTTVIQDTAFFNQMISSSQTLNPSYGYLWWLNGKSSFI
                     APTSPLSIPGPIAPDAPADVVVAGGSQGQLVSISFSKNMVMIRQGVSGEEDLVPLDLH
                     NEIWKKIGELNCPSTPVHQLTRKTIRIHPNPARDKVRISMSDARGMEARIYDLNGRRI
                     KIFQNREDLDLEGLHEGAYLLEIIKGGSRWFEKIILSPGY"
     gene            44379..44699
                     /locus_tag="PKM27_03455"
     CDS             44379..44699
                     /locus_tag="PKM27_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR06344.1"
                     /translation="MKQDLFQAIADPTRRAILSLIAVQALTPNAMAEKFDMSRQAVSK
                     HIKVLHECELIKPEYAGREIYYHFNPKKMHELDLWLDQFRKIWETQFDQLDQVLLTLK
                     KRKK"
     gene            44696..45226
                     /locus_tag="PKM27_03460"
     CDS             44696..45226
                     /locus_tag="PKM27_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNR06345.1"
                     /translation="MTNSLFFDFTVDKAAKKVFITREFDAELSLVWEAFTVPEILDQW
                     VAPKPFTARTKYMDFRVGGRRFYAMVSPEGHERWLIQTYTSISPKTHFKLFNAFADKD
                     ENPELPGSEWDYSFSEKDGKTTVRVTIYNDSLARMEKMIELGFKEGFTASLANLDLAL
                     ESRKRSESLKMDEMKK"
     gene            45257..45682
                     /locus_tag="PKM27_03465"
     CDS             45257..45682
                     /locus_tag="PKM27_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNR06346.1"
                     /translation="MDVKEQIKNHISDQAEPKRSELQELHRRILQIAPKTRLWFFDGK
                     NTENQTVSNPTIGYGFHTMKYANGKTADCFKIGLSANKTGISVYILGIQDKTYLAKTF
                     GEKLGKAKVTGYCIRFKALKDINLEVLEEALRYGIETAD"
     gene            45846..46409
                     /locus_tag="PKM27_03470"
     CDS             45846..46409
                     /locus_tag="PKM27_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR06347.1"
                     /translation="MKKHKMIFWTAITVAVTFLTTSCFNNMKHNTMAETNQKITPYLW
                     VEKDAKAVADYYLSIFKNGKLKDYRKFNIDESGNDAGIETAVIEIEGMEFSILAAGPL
                     FKFNEAVSFVINTKDQAETDYYWDALTADGGEEGSCGWCKDKYGLSWQVVPTEYFELI
                     HNEDPKIREKAMKNTLKQKKLILAELK"
     gene            46497..47150
                     /locus_tag="PKM27_03475"
     CDS             46497..47150
                     /locus_tag="PKM27_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR06348.1"
                     /translation="MRFYKNGLFNKGTMTGPVLMILCLTIAVIPIDGFSQTKRLWTES
                     DRDFLVSHLERTKKEIINETQNLTLRQWSFREDSAKWSIGQVLEHLGLYERIFMQEAD
                     IMLSTNPEPEMDKNALPDSSYIHWMNDPSPHKAEWNAEPLGFMDGKENLNFFLFGRDR
                     IIDFIRNTTYDLKAHYTFRWGSEKRRSIHALMVVHFAHTDRHLRQISRIKLSKNFPE"
     gene            47834..48232
                     /locus_tag="PKM27_03480"
     CDS             47834..48232
                     /locus_tag="PKM27_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06349.1"
                     /translation="MKQLTAIIERNKDAFFAYIKEIDGCVAGGKNYQEVKLNLDKMLT
                     IAQKEDKDLKKILSRGYKLKFEVDLESVFDLIPEVNISQLAKTGNLNPGLLRQYVSGT
                     KKASEAQTEKVMKAIKDVSMKLNSITLTVS"
     gene            48408..49544
                     /locus_tag="PKM27_03485"
     CDS             48408..49544
                     /locus_tag="PKM27_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR06350.1"
                     /translation="MEITLPISSLIVIAIGSVGIAVSIFIGIMLQSQKDKKHISISLL
                     ACLLILSGLTLLNDVLVTSGISNRIKQLYFVPIYYSLSVAPLFYLFIKSKFSYRLNKS
                     DLLHLVIPTIQAFVYFSIGFRSVAFKSMLYDENAFRMYLQIESFLFPIFLVGYTVLAL
                     LFLQGQDQEKYFWTKDIKKWLSNFSAGMLLIAIMEFCFSLMEYNTSANPLPGFPFYLV
                     HTFTLSAFVFWISINGFKQYYPLQIFISKPSRQEANIDEYELSKLLEKLKLLMTNDKI
                     FLNPDLTLELLSNYLGISEKQCSSILNKGTNLNFNQYVNEFRIKAFKEKIKEGQNKTY
                     TLTSIAYECGFNSKSTFNRVFKSTCGVTPSEFVKMTTQDTTVSD"
     gene            49660..50535
                     /locus_tag="PKM27_03490"
     CDS             49660..50535
                     /locus_tag="PKM27_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNR06351.1"
                     /translation="MNSKIKAFLFGGNHQNSISQNIGFLLLRFFVGLALCTVFEKFFP
                     KNGIWGPQDWFIQDTANMGFPFPTFFAWAAVISEFFGGILLMLGLFTQPAALLNILVT
                     FTATFIYHKGDIGNAGLLSFFFMIMCLCILLFGPGKFSLDYYINKRMAKRMTTITTIV
                     LLIFSFNFGEAKANRQSIPYQIEVLIADSSQIRFCLKNNSLFPKKRIFIIYQLQQGGN
                     NTIVRWFLPFQKIQFDVPIGSKIYIAADSQVEKVMQGQRIDSDKPFLTEDDRINNITL
                     QSQDSVKFKVLFLSS"
     gene            50629..51441
                     /locus_tag="PKM27_03495"
     CDS             50629..51441
                     /locus_tag="PKM27_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR06352.1"
                     /translation="MTHSTDIYFPTTETEKYFIKSIWRLQEFNNHNLTETILPKGTVE
                     LIFNLSDKIIYLNSVADVRTTLPPCFINGINFKPFQLIKNGQQLFVGIQLNVIALKAL
                     FEIPAMEFNDAIVESSQVCKPLSDLYNQLFSERAFERQVQIIRKWLYHKISTSRYLAA
                     LQKFYNWFYFPNVNTMTVKELGNRACISERQLRRITTEWLGMNTEAFLLYNKYLSSLH
                     LLHSSDLSLTQIGLEAGYYDQSHFIREFKSYTDLTPKEYQASVTGLPGHIFG"
     gene            51526..52227
                     /locus_tag="PKM27_03500"
     CDS             51526..52227
                     /locus_tag="PKM27_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06353.1"
                     /translation="MMNAVKFKRTVGGITVLSGIIGFTSYFLVAGSVSFNFEFFSNPS
                     LIFSMPDVNIVMLRWSMITDLFGYYLLLLPFIFFMHEWLLNKSAWRKLFTFCGTAYVM
                     AGAIGAAILAVTWPAFLTKYPGSPHEQQETIKLLFESFSLMVGNGIWNLFDTLVCGVW
                     MIGIGVYIKRVKSFLGWFTVAVGIISLLDGFGNMLELSEVSDLALNLFLFLAPLWAIF
                     IGFAVMRNSFSLEKS"
     gene            52240..53277
                     /locus_tag="PKM27_03505"
     CDS             52240..53277
                     /locus_tag="PKM27_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNR06354.1"
                     /translation="MIYKFSLIEKMLLANDVIPHPFADASFSVGLGFALGSSIKLKIA
                     DQLSMEYKEVGTIAHAANVSEVGAELILDCLDALGYVQKKGSHYAFTKRGYKNLSPKS
                     PHNFRYFILFCDYLYKGYINLDETIRLGRTANSNMLEQMTDYEWELFSRAMIDISSTN
                     YKEVAGKIPVAKSAQKMLDLGGSHGLYSIELCNRNPNLKSTIVDLPPVKKYADECIAQ
                     YNSGKTVSFLPADFMKDELPKNVDVILAFNIIHGLNPSENETLAQKVYQSLNSGGIYV
                     ILDQIKGIGGSSQMSKATTSYMALNLLHQAGGKTYSKNEVDTFTKKAGFKNTVLKKLN
                     APGFGVIICTK"
     gene            complement(53759..54874)
                     /locus_tag="PKM27_03510"
     CDS             complement(53759..54874)
                     /locus_tag="PKM27_03510"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR06355.1"
                     /translation="MNIRTIISGLIISLTIVSCNWDKFLFPSGPKFNIELFEQSLLDS
                     MTGQVPGFAYTINQNQIMYSMGVRGFADEANRIPFTPVMRMHIASVSKTITAAAVLKI
                     LARKPGANIDSKISRYLPSGWVRGPNVDLLTFRHLLTHKSGFIQSGGNDEYGALRSMI
                     EGGINKAALGNHYEYKNANYSLFRVILPILAGKKDKSQIDAFGAAVFYSWVIHEEIFK
                     PMGIPDAFVFQLPDSPMKYYNCVSDVTGGGGDYRMRSGAYGWHLSAFDLAAFMAHLRY
                     NNDILAPVTRKQMDDGLLGWENEHGSANGEHGTYLYHSGGWHAGDDPAPGLRTCIMKY
                     ADTKVEVSLLMNCVDHPNPNIASPRKLLRIIFDNSWE"
     gene            complement(55345..57519)
                     /locus_tag="PKM27_03515"
     CDS             complement(55345..57519)
                     /locus_tag="PKM27_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNR06356.1"
                     /translation="MKMKMTWVKFLPVNTSVTPLFLVLVASAIILFSCKADRGEKYMV
                     WDTYRGSPDALQYSALHQIDTSNVHLLQPAWTFHTGDSGNRTTIECNPIIIGDRMYIT
                     SPGLDLIALDAASGQEVWRYEPAESATGGGINRGLTYYKTKDKACLFLPVGHYLYAID
                     AGTGKLVESFGDYGKIDLRIGLGKDTAKLSVSLTTPGIIFKNLIIIGSATGEGYDASP
                     GHIRAYDAGTGEMAWIFHTIPGQGETGHESWAWMEGENYGGANNWGGMSLDEKKGVVY
                     ASTGSPTYDFYGANRIGQNLFGNCILALDAASGVLKWHYQAVHHDIWDYDLPCAPTLA
                     DISWEGRSREVLLQPTKMGELLVLDRHTGEALLDPEERSVPASGVPGEAAFPSQPFGQ
                     GIRLTRQGWDTSYLTNISDSARAFVLKESAQYQGGGLYTPPSLQGTLGMPGTRGGMLW
                     GGISFDPVQRIAYANCNDFAMIFQLQKVVPPGGGEASLLARGYNTYMLNCTSCHGADR
                     KGQNQAVPMLTGLGKKYSKSALTGVITRGKGLMPAYSQFSEPELDALAAFLLDENTGS
                     GDHSTVNPDGPGERSERFVLRSYKILTDKDGYPGVRPPWGTLNAVDMNTGRLKWQVPL
                     GYYPALKEKGLGATGTQNFGGCAVTAGGLVFIGATADERFRAFRASDGKELWSFQLPA
                     GGYATPSVYQVKGKQYVVIAAGGGNRNGTPSSDVYLAFALTE"
     gene            complement(57516..58781)
                     /locus_tag="PKM27_03520"
     CDS             complement(57516..58781)
                     /locus_tag="PKM27_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06357.1"
                     /translation="MIRRSILTFAASAILFHQIPAQDPANYDEDRAGQYVLPSLLVTE
                     EGHPIRSAEEWEKKQRPAILQKFATHVYGRMPGRPKDFHFKENALEPNALDGKAIRKE
                     ITLYFTKGDQGPALPLLVYLPKNHKGPVPVCLGLNFQGNHSTQTDPGITITDQWKSLH
                     PGTTNPERGGQARRWPVEEILSNGFALVTAYYEELEPDLPEGWRTGIRTTLSKELEIK
                     LHEWGAIGAWSWGLSRIMDYLESEPAVNPAQVVLTGHSRLGKAALWAGANDRRFAIVV
                     SNDSGEGGAALARRNFGETVQRINTAFPHWFIEKYKTYNHAVDQLPVDQHMLLALMAP
                     RPLYVASASEDLWADPKGEFLGALHAGPVYALYGQKGLMTEQQPPIGQPVGETIAYHI
                     RAGKHDILLYDWKQYLRFASRHFKTGQTK"
     gene            58926..60002
                     /gene="serC"
                     /locus_tag="PKM27_03525"
     CDS             58926..60002
                     /gene="serC"
                     /locus_tag="PKM27_03525"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNR06358.1"
                     /translation="MKKHNFNPGPAVLPAPVLQEASQAILDYNHSGLSILEISHRGSD
                     FVAILDEAISLTKELLGLSDDYHVLFTTGGASSQFFMLAMNLLNEGDTAAYTDTGTWA
                     NKAIKEAKAYGQIKVIASSKDKNYSFIPKDLGALPADARFLHLTSNNTIFGTQIHSWP
                     EVPVPIVCDMSSDIFSRTIPVDKFGLIYAGAQKNAGAAGVTMVIIRKDMVRTDLTHHL
                     PPMLDYKTFISENSLYNTPPVFPIYVSMLTLRWIKKLGGLEAMEKINRHKAKLLYDEI
                     DANPLFKGTVAVEDRSLMNVCFVAENETLENEFAAEAKNAGCVNLKGHRSVGGFRASI
                     YNAMPTESVQVLVDTMKEFARKKG"
     gene            60018..60755
                     /locus_tag="PKM27_03530"
     CDS             60018..60755
                     /locus_tag="PKM27_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNR06359.1"
                     /translation="MVPLQEETYIEYTGHRIPLVIIRERRRTVRVAFSRKTVTLRLPW
                     TLAASENNKHIENSKKWVIDQLKRKPGLIHHFYTRIYRHGETIRVAGRQYVLYMLEHE
                     GKTSKAQLKGQLIELKLAPGKEEEKNEIIKYLVSRLIANDCHEMVEARVRELNRMTYN
                     RPIQTVRLKLNQSNWGSCSSKGNINLSARLLFAPREVMDYVIIHELAHLVEHNHGPRF
                     WALVAKAMPGYQSCEQWLKDNSHLCEF"
     gene            60939..62477
                     /locus_tag="PKM27_03535"
     CDS             60939..62477
                     /locus_tag="PKM27_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNR06360.1"
                     /translation="MTAMQAISPGWILVLILGYFVMLIGIAWWTSRGSASNENFFLAG
                     RNAPWYLVAIGMIGASISGVTFISVPGFVQANQFSYFQVVIGYIFGYMVIGLILMPMY
                     YRLNLTSIYGYLEKRFGWKSYKTGSAFFLLSRSVGSACRLYLVALVLYQFVFGPLGLP
                     FWLTTLITIFLIWVYTFRGGIKTIVYTDVFQTFFLLASLIISVILISKSMGWGPGDLF
                     TNVRESEYSKTLFFDSGWSDNKHFIKQFLGGMFITIVMTGLDQDLMQKNLSCRNLGDA
                     QKNMFSFTIIMVIANALFLVLGALLFLYAGAKGIEVPTREIAGVMKAQPDLLFPTLAL
                     QHFGGIIGIFFILGLTAANYASADSALAALTTAFCVDFLGFERDRVAGDRIVKVRTRV
                     HFAFSMILFLMVLVLWFMNDRSVIDTVLTLAGYTYGPLLGLYAFGFFVKRQVRDPWVP
                     WICVASPLITFILNKYSADLFWGYKFSFELLILNGLLTFAGLWLVSYPSPISPNLLGY
                     KNRV"
     gene            62474..63292
                     /gene="murQ"
                     /locus_tag="PKM27_03540"
     CDS             62474..63292
                     /gene="murQ"
                     /locus_tag="PKM27_03540"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNR06361.1"
                     /translation="MTDRITEQSSHYRHLEQMSVEEILTNINREDQTVPLAVEKSIPQ
                     IAALVEAIVAKMKEGGRLFYIGAGTSGRLGILDASECPPTYGVPFDLVIGLIAGGDTA
                     IRRSVEHAEDDPKQAWLDLEAWDISAKDIVVGIAASGTTPYVVWGLSDCRAHGILTGC
                     ITCNPGSPLETNADFPVVAVVGPEFVTGSTRMKSGTAQKLILNMISTSVMIRLGRVLD
                     NKMVDMQMTNVKLKDRAAHMIVQKLNVPYEEAVEKLRVHGSVRAVLDEYEKLRN"
BASE COUNT        16964 a        14426 c        14548 g        17332 t
ORIGIN      
        1 gaatatttta acaatctggg aaacctgaaa aaattgcata aaacaaacat ataagacgca
       61 aagttcgcga agagtgtgga gttcccacaa agacgcaaag atcacggcac gaaggttttt
      121 tatattattg ataatcaata atttgtgttc attgtgcctt tgtgggagaa aatattttgt
      181 gtccttcgtg gcctttgggg aaacattggg aggatgccta ctttgtgggc cctacaccgt
      241 agctgttgcc aaaaacaatt ccgcctggtc atccatggct ttgatcttca aaacattaaa
      301 acaaggcagc caaacagcct gccccttgcc aaaaacctgg ccctccgggg tttcgacctt
      361 gcctgaatgg atcagcacga tagaggccgt attgaactgg atggttttgc tgtcgcctgg
      421 gttcagttta aaatgtttga gcaggaaatc gggcacagga ggggtgaaga ccagctcgtc
      481 gtccatgaga tagtccggct ttaaaatatg ctgtcctgcc cggctggtat cggcatgttt
      541 caaaaactcc cggatgtcaa tatgtttagg ggtcaatccc ccgcgcagga cattatcgga
      601 attggacatg agttccacat tctgcccttc caaataagcg tgaagcaccc ccggcgcctg
      661 ataccctgcc tggcccggac gtaaatattg aatgtgcaga aggagcatga ccagtaatcc
      721 gcggtcatat tggcccttta ctgccggata cgtctccata gcccttgcaa gccaaaaacc
      781 aatattcttt ttgtttgctg tttggcctgg gcttaacctg gaggccaatt gggagcagga
      841 gtgattcacc atttcctggt ccagggataa aaagagttcc aataaaccct tccagtccag
      901 tccaccggtc cttccttcca gttcaggtaa ataaaggctc attcgctgat ggatatgttc
      961 aagcggtgca aatccctgga gcatccagaa atcggaaagg gcaagaagca tttcgggttt
     1021 gtggttgtcg tcccggtagt tgcgttttgg atcattcagg gctatccccc gattattttc
     1081 ttcttcaaat cccacctccg cccccttttt atccggatgg agctggatgg aaagcatatc
     1141 ccggacatcc agtattttca gcaggaaggg caggcggggc ccataggtgg aaaccgattc
     1201 ggcacccagc tccttcaagg gatctgcctc aatccgttct ttcagggaag aacgttcgtg
     1261 gccgcttaaa atcaaagaag gatgattggg gtgggctcca aaccaggcct cagcaaaaag
     1321 ttggtggccg ggattttcaa tgccgagcca ctggggaagg taggaaaagc ctccccaggc
     1381 gtaatgctgg aggtatggtt ccaggcgctc gaaggaagac attaggaatg gcgcatttgt
     1441 ttataggcat tgatcaggcc cctggtggag gcgtcgtgtt tttcagtggg ctgctccgac
     1501 gccaactccc cgaggatttg agtagccagt tttttgccca actccactcc aaactggtca
     1561 aaggaataaa tattccagat ccaaccctgg gtgaaaattt tatgctcgta cagggcgatg
     1621 aggctgccca gagtttgtgg gttgagtttt ttgatcaaaa tggaattggt gggacggttg
     1681 ccctcaaaaa cacggaaggg cgccaggcgc tccatctcgg caggagacag ccccgccttt
     1741 ttcatctcct ctttgatttc ttccagggac ttgccattta aaagggcttc cgtctgggcg
     1801 aaaaaattgg ccatgagttt gggatggtgg tcggaaaggg gattgtggga ctgggccatg
     1861 gcgataaaat cgcaggggat cattttggtg ccctggtgga tcaattggta aaaggcatgc
     1921 tggccattgg tgccgggttc tccccagatg atgccccccg tctggtaatc aatgatctgg
     1981 ccgttccggt ccactccttt gccattgctt tccatattgc cctgctggaa ataagccggg
     2041 aatcggtgga ggtactggtc ataggggagg atggcctcgg attcggcctg gtagaaatta
     2101 ttgtaccaga tgccgatcag ggcgagcaat accgggatgt ttctctcgaa cggggtgtta
     2161 cggaaatgca ggtccatggc atggaagcct tcaagcaatt ccctgaaccg ttcaaagcca
     2221 atcgtgcagg ctataggcag tccgatggct gaagtcaggg agtagcggcc accgacccaa
     2281 tcccagaacg gaaacatatt ttccgcggag atcccgaaac cggtgacttc tgcagcattg
     2341 gtggacactg ccacaaaatg tttggcgata tggacttctc cggcctggtg cacaaaccat
     2401 tgcctggcgc tgtgggcatt ggtcatggtc tcctgggtgg taaaagtctt ggaggaaata
     2461 ataaacaggg tggtagccgg cttgattttc tgaaagacct ggtagatgtg gctgccgtcc
     2521 acatttgata caaaatgcgg ggtaatgccc ctgtcccaat agggtttaag cgcttcggtc
     2581 accatgacgg cgcccaggtc ggatcccccg atcccgatat tgacgatgtc cgagatggtc
     2641 gaaccgtcgt atccggtcca ttggccgcgg tggagttttt cgcaaaaggc ctgcatctgg
     2701 ccgagcactt tgtatacttc gggcatgaca ttctccccat cgacccagac cgggctgccc
     2761 gaaatgttcc ggagggcggt atgcaatacg gcgcggcctt cggtggtgtt gattttttct
     2821 ccggtaaaca tggattcgat gccttcaccc agcttgcact cctcagccag ttcgagcagg
     2881 agggcgaggg tggtttcgtt gatcctgttt tttgaaaaat ccaccaggaa atccccccag
     2941 cggatgctga atttttcgaa gcggtccggg tcccaggcaa actgatcctt cattcggagg
     3001 tcccgggttt ccctgaaatg ggttttgagt ttttcccagg cctgggtttt ggtcgggttg
     3061 atggcggcta tcatatttta tttaactaat ggggcaaaag taataagtta gacggctaaa
     3121 atgtccagga atcagaaaac cggcaaggaa ggggaggacc tggcggcccg tcatctggaa
     3181 ggagccggat atgagatcct cgaacgcaac tggcggttca gcagggcgga gctggacatc
     3241 gtttgcagga aggagggaat cctgatcttt gtcgaggtca aaacgagatc atacaccttt
     3301 tttggggaac cggcggagtt tgtccggagc aaaaaggaaa ggctgatcat ggatgcggcc
     3361 aatgcctata tggcctccat cgggcacgaa tggcaattcc ggtttgatat tatcagcatc
     3421 gtgctctccc cggacggcag gcaccggctg gagcatgtgg aagacgcttt tttcccgggt
     3481 tggtaagaat taagtgaagg gttgactcaa aatcaaccct tcacttaatt cctttttgag
     3541 aattaaccgg gaacccttat atttgcgctc caaattttag cgaaagatga aaaaagacat
     3601 tcacccgtcc aattaccgca tggtggtttt caaagatttc tctgcggacg aggctttcct
     3661 ttgccggagt tgcacaccta ccaaagacac catagtatgg caggatggta aggaatatcc
     3721 cctgatcaaa ctggagattt ccaacaaatc gcatcctttt ttcaccggca agatgaaatt
     3781 tgtggacacc gccggaagga tcgacaagtt caacaagaag ttcaaaaaag cctaaggctt
     3841 taataagtca tagataaagc gaagcctggc aggaatgccg ggctttgttg ttttggggtc
     3901 ccggaggaaa tttgaatata aacgaatacc atccatttca taggctgatg cggtaaacat
     3961 tggttttcct tttttcaaag cccaggctcc ggtaaagttg attggcttcg atgcgcgaag
     4021 gcctggaagt caggtcaata gtatggcccc ccagagcccg gacctgttcc atggcatgtt
     4081 ggatcagggc cttggcaata ccctgtcccc ggtatggact gtccacgacc aggtcttcga
     4141 tccaaaaccg gatcccgctg ggtattttat accagaccag ggtcaggcag cccctgattt
     4201 cgccctcttt catggcaacc agcaggcggg tggtggggtg gtagaggatg tcccggaaat
     4261 gggtcagggg atgggcggga cactccgggc agagttgggc catcagctca ttgaggcggt
     4321 cggccccgac ttggtcgatt ccagtcagta attcaacttt taccatggca ggattaagat
     4381 ggcgaggatt ttttactttc actaaaaaaa ttatctttgc actcccaaaa aagacttggg
     4441 cgaccggctt gtttgccgta caggaccggc cggattatga aattcagtcc catagctcag
     4501 ttggttagag cgctacactg ataatgtaga ggtcggcagt tcaaatctgc ctgggactac
     4561 ccattcaggc caccagggtt gcataaaaaa gacttagttt taccggcctg atcgaaagaa
     4621 aggggaatta gctcagctgg ccagagcatc ccgaccgaag cgtcgggagg gtcaagnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcaa gcggggctac ccaaaagaga
     4801 aaattgaaat gaaggggaat tagctcagct ggccagagca tctgctttgc aagcagaggg
     4861 tcaagggttc gactccctta ttctccaccc tattttaagt ttaaaacctt gtatatcaat
     4921 gatatatgag gtttttttat tttttggatg tcgtattggt tgtctaattt tcaactttaa
     4981 ctaaatataa attgctcggt aagactaaca accattttcc gaacagtgcc caattattca
     5041 atgttgccga ggaagaattt tccatcaagc tgaaagttaa ttaaattaac ttaattggga
     5101 ctgaaagtta aaatgcagat tgtagaggtt catagggggg ataatcgaaa aatcggatta
     5161 accggagggg aggaaagatg gatctacctg agaccatgca tatgaatgaa tattttaaag
     5221 ataatcgtat agaatttgcc tttggaggca gcaaatagaa ctcccataaa tacttccaaa
     5281 atcaaaccaa ggaggtgatg tcattctggc tgagcacatt tccgaattct taagacgagg
     5341 tttcaatctt attgatgaaa gaataattct aattaagcag tataagacac aggctgtcat
     5401 ggagatagat aatttgaatc attcgaacaa aacccactaa atttgaatat aacaacttat
     5461 gtactatggg attatattca atcctgttat cgattgtatt atttatattc tcgatcgatt
     5521 ctaaatcccc tgaatctgct tattatttga tttgcactgg aaaatatgcc aaatcccatc
     5581 acagagatta taaaaatcct tctcaatatt gtagaggact gaatgcatgt aaaggtgata
     5641 tcattatatt agattctgca catgccgtta ttcttagacc cgatgcttgt gattattgtt
     5701 acaaataaat ctatgattct atgcgaaaaa tttccgctta tgtttttacg ccagtagtgg
     5761 gatatatttc ttacagtata gcagtcatat tgaattcaat tcttgcagag gattggtctt
     5821 cttcgattcc tggacttgga ttatttatat ttgggaatat ggctgctatt gtagggatgg
     5881 caaaatttac cagttggtta actccatccg acaatttgcc ttatatagta agcgtactcc
     5941 tatacatttg ctatacaatt tggggtatgt atttaaatcc tcaattcaca attctttatt
     6001 atttaataat attattgact ggattcggag ctttggttta tagtcaacac gagatgaaaa
     6061 ctaaaaatat tagtgatgaa gatgattcat gtgaaaatac tattagtgtt gattccaatt
     6121 ctcaaacaaa acttccttgg cctaaatatg aatcagggcc aaaagataaa agacaacaaa
     6181 atgacgattc ggataacaat atcaaaattg tcctgctact ttcaatgcta ttggtaggat
     6241 tttttattta tcaattccta aatgaaaaag acaataacaa tcataataaa ccctctgctg
     6301 aaaaatctaa gcctaacgaa attcccaata atccaaatca aataaataat ttaaatgcaa
     6361 atcttaatgt tcttgcagat aatagaccaa atagaacgga gcttaatgca agcatgaatg
     6421 aatcatcaga cccaaatgaa tttatattgg ctttcctgag atatattaat gccaaaaact
     6481 ttgacaaggc atatttaatg acattaaacc caaaatggga gccaaaagat tctttttcaa
     6541 cacaaggtat ttggggagga ttccgtgcat tggaggacat tgttatagat tctgatgtgg
     6601 gatattcatc tggatttggg gacaaagtac taagagtaaa atataccgcc tatggagtaa
     6661 atgcagcata tccccagaaa tatgaatggg attatcattt aaagaaaatt gattataaat
     6721 ggcaaatcat taagatgaca ttgcctaaga ttactgacta taaattaggt aacattaaca
     6781 gccctcttga tgctgtgaat ctattcttta gaagtcttga acaaggtgat ttttcagtag
     6841 catttgcttt aacacgcaat cctgaatgga aaaaattttc aatatttatt caaagctggg
     6901 gaacgatatc tgatttgaaa gtgtatgaaa taaaagagga tattgggaca ttatcgccaa
     6961 gttttaatgt caaatactat tcaaataatg caaagactgg attatctggc atctttatat
     7021 acaacttcca gttagaagat attaataatg tttggagaat tactaaagtt ataaagctat
     7081 ataactaatc tatttcgcta tgtgattata tatagtattt atgattatat aaatagccaa
     7141 cattatgtta taattgtttt ggacctagtt aaaatatttc gtatacattg aataataata
     7201 aattagtttc agagttaaaa ttaatataat gagatattac cttaagacac acttgttaat
     7261 attggtctac ttagtattac attcttccct ccaatcccaa acttataaag aattatttta
     7321 tgctggtagt attaaatatc agaaaggaga ctataaaggg gcaattattg actattctaa
     7381 ggcaatttta ctaaattcaa ataatttcga gccatttctt ttaaggggat tagcaaaaat
     7441 agagttgggg gattttagag gagccatttt agattataac caagcaattc aaataaatcc
     7501 taactacgac gaggcatata ttaatcgtag ttatgcaaaa gcaaaattga atgatttcac
     7561 gggacaaatt cttgatttga attttgcaat taaattaaaa ccttccccag aaaattatat
     7621 tgaacgcgcc aaagcataca taaaaacagg taataaggaa aatgggtgtt tagattttag
     7681 taaggcaggc gaattaggtc atcctaatgc gtatgaatat attaaacttt attgcaacta
     7741 agattagttg attgatcgtt tattcttacc ttaattcttt aaatgattaa taagagaact
     7801 ttaaaaaatt agagagaggt tagagttata aagttattta aatattaaca aatttctttt
     7861 ataatcaaat tcataagccc caaatctttt taaaaaactt tgacctaata gggatgaaga
     7921 attgggttta tttcctattg cacaaataac attttgtatt gttttatttc caattcttat
     7981 agattttaat agatattgct gtaatgactc tatgcttcca tcggcatagc tataggtgct
     8041 tttaccaaca taatccgatg cagacaaagc tcccgatttt cttaataaat ctgcaatttc
     8101 tggagagaga attacatccc cagcacctgt atcaaatgta attcgagtct ttaaactgcc
     8161 gtttagctcg ataacaactt ggtaaattcc accaggtcca tgataaatgg gaacttgctc
     8221 tgaagaacct attttatcct cataatacct attatttcta acttttatat tagtttttaa
     8281 cttaatatcg tgtccagaat tggatgaatt gagatcagta accccaagaa attcaatatc
     8341 aatttcttga agtatgtatt tataatcaga aattagtttg tcgcttttat tgttattttt
     8401 gtaataataa tagtattttc cccagcctaa gtctttatca tcttttgtat attcaatata
     8461 aaaagtggga ttacttaaga ttttataagg acttagtatt tttatagttc tcgaatacaa
     8521 actcagtata tccatagctg atccaaaagg aatgtactcc tcataactaa ttttaattac
     8581 ttcaagagga gtcgaaggca ggctaccgta tcggggctct agatatgacc aaatataatc
     8641 ctctatcgac atgtattcac caacggtgtt tcgatataac ctgaggaagt cttcatctgt
     8701 tttcatgatg ttcaccattt taacaaattt atcatcatta tatggagatg taggtattgg
     8761 tccaaggtta gccaagttaa tatccttgac aattgaaaat gattcatttg caattttagg
     8821 ctcatattca tttggacata tacggcatat taacttgact tcagtttttt taatatatgt
     8881 caaattctca agaatatttt cagaaccgtt tttgattcta ggaataacag tgttcaattt
     8941 gaatccatta tcttttaata tctcagaaat tgaatgataa tctccattat tataaaattc
     9001 taatattaga ttgagcttat ttaatggagt ttgagaatta actatggtag tagtcaaaca
     9061 cgttattatg aaaatcaaaa gtttcatatt atcttaatta tggtatttat aaaaataatg
     9121 taaatgtatt ctaatacaaa attatcctac tctcctctcg tataaaaact tctgaaaccc
     9181 tataaacaaa tccttcacct tcgtcagatc cccaagtttc cccccggcat ccggaaggga
     9241 ttgatattta gaagtgagaa gtacaggtag tttatcttgg tcgagttcct ctactcctgt
     9301 ttcaacgtac ttactcaata caaactcaat aaactctttc tgcctgtcat tgagcaaggc
     9361 aaagatagta gcctgggctg caagaactct tgcttccctg gtgataggtt tgaagtcact
     9421 gttaaataca tattccagta catcaaagag atcgctcttt tcggcatcaa taagcttttg
     9481 caaggtcgtt agttcctcct tcccaaagcc agcttcgtcc agtttctcca ataaagttct
     9541 tctggtcaaa ggattgctcc aaagggttcg aagttcttct tcgcttttaa agaattcagg
     9601 cagggaaccg aataggtttt gcagaaactc ctcaactgag ataggttttc catctgaact
     9661 caaaaaggat gtagaaatca tatgttgaat ttctcgttcc tttccatcct ttaatttgat
     9721 tttaatcttt tgtggaagtt cacgcttgac ctcttcttcc tctggttcat gaaggtcctt
     9781 tggtggattc tcttctccaa ttccttgtcg ttttggtaca tctggttcta caggttcacc
     9841 atcccattcc gggtctgcaa aatggtgata ggcatcgaca aaatcgtaaa tggtaaaaaa
     9901 ctcctttccg tcaaacaatc gggttcccct gcccacaatt tgtttgaatt caatcattga
     9961 gtttacaggt ctcagaagca cgatattccg gatatttcgg gcatctactc cggttgataa
    10021 cttttgagaa gttgtaagga ctgttggaat agttttttcg ttgtcttgga attctctgag
    10081 aaactgatca cctatttccc catcattggc cgtgactcga acgcaatagt ttggatcttt
    10141 tgattttttg tactggttta caagatcacg gatgatggct gcatgctcct gcgtagcaca
    10201 gaaaataagt gttttttcgg actggttgac ttcatccagg tatatcttta ccctctttgc
    10261 ttcccgctct aagatttcta tcttcttgtt aaaatccggt tctgtataca gtttgtcttg
    10321 ttcaatctcg ccttcaacaa tttgatcatc ggaggtataa atataatcgt caagagtggt
    10381 ttttattcgt ttgaccttga atggagtgag atatccatca ttgattcctt cctttaaaga
    10441 atagatgaag actggatctc caaaatattt ataagtatcg gcattgtcca atcgttttgg
    10501 ggtggctgtc aatccgagtt gaacggcagg ggagaagtat tccattattg cccgccaatt
    10561 gccttcatca tttgctccac cccgatggca ttcatctata acaataaaat caaaatagtc
    10621 aggagggtaa tcgccaaaat agggagcgga ttttccctca ctatccgtac cgctcatgaa
    10681 tgtttgaaat atggtaaaga aaatgctgcc atttgtgggc acagctcctc ttttcctgat
    10741 cgcatccggc tttattctta caagggcatc ctctggaaag gccgaaaagg cattataagc
    10801 ttgatctgct aaaatatttc tatccgcaag gaacaaaata cgaggccgtc tacttccgtc
    10861 gtattttaaa ttccacctgg catgaaagag tttccaggct atttgaaaag caattgccgt
    10921 tttacctgtg cctgtagcca gggtcaataa aatacgattt tgtttattgg caatagcctc
    10981 taatgtatta ttgattgcga tttcctggta atatcgcaaa tgccaagtcc cacttctgtc
    11041 ttcaaatgga attgaagcga atttctccct ccaaagattc tgattcgcaa aggttttatt
    11101 ccaaagttcc tcagggctga gataattatc taccaaaccc tcaactccag tttttaaaga
    11161 gatactataa atcgatttgc cgttagtagc ataggtggtc tccaaatcca tttttagagc
    11221 gtattgtttc gcctgggcaa ctgcttcacc tacttccagt tcatcacttt tggcttcaac
    11281 tatagccagt ttaagacctt tataaaccaa tacatagtct actttcagtc ttttggctct
    11341 tatacctccg gtttgaattc taccagccgt gatatagtat tcacgaagta ttttggaacc
    11401 ttccacaacg ccccagccac aacctttaag cttggggtct atgagttctg ctctggtttc
    11461 ggcctcattc atatatttct tctattaagt tctatcacat aaccatcaca taatatgtaa
    11521 ttgataatca actatataca ttacaaaaag tagcgttcta tcacataacc atcacatacc
    11581 aaacttaagc ccaaaatgga atataacttg catattttct tgatttgctt tccggatcat
    11641 cttctttgat cactccgtcc actacagcat ctcttattat gcgagaagca atagaataat
    11701 tgtgatcttc gattttaaat cgctgtctta gactctgatt tgtcattttt tcgtttgaaa
    11761 catacttaag acaagcatgc tgataacatg cgcttatttt ttccttttta tccaagtcat
    11821 ttagagattt atagcaatac atcgttaccc ttgtcctgtt ttccgaggcg gtaacgttta
    11881 taggtggaag ttgaaacaat tcgttataaa aaataacttt atctaatcca cttcctttct
    11941 cttcacaaaa tcccattctt cgcatgagat cagctaaact ttcatttcta gattgatagg
    12001 catcaataaa tcgttcaggt ttaacaagtg gagttccagg atttgaaatt tcaattctgt
    12061 cactaaaaat ctcaatcatc ggaaagccct tttcagccaa atcttgatga atgagtgcgt
    12121 ttgcaaccaa ctctcgaatt gcaatttctg gatacattct agtgtctttt ctcaaagcct
    12181 ttccaatttc ttcatttgca ggtaattgac tattaatcca atctaccaat cctacaaaac
    12241 ccaaagcata acccttaaca cccatttgct ctcgttcagt ttcaaccttg tttttacctt
    12301 tgtagactat tacgcgaact gattttcgtt caacgctttc aaagtccttt aattgttttg
    12361 caaataataa agctcccaac tttgtaatgg catatccctt attcttgaca actatcccct
    12421 cttcaattaa cttgtcaatt acagcttgtt gtatggatgg atatggtagt ttcatcaagt
    12481 caaaatatgt ctcagtgcta aggtacttga taatatcaga ggcgactaaa ttatctctgg
    12541 caatttcctt ttcaaaaggt ggaaagtctt ttttccaaat cttagcttgt ttttcgggaa
    12601 actcattaag tttccgaata attgtgttta ttcttatata tgcttgatga agaaattcaa
    12661 caggtcgatt ttttgtagca ggtataatgt aaatagaaat atgacgatta acatcataat
    12721 caaattcaaa taccaaaaag tcaattcttg gatttaaacg ggtagctaac caatgttcac
    12781 agtcttcatt tcccttttta tattctttag ccttaaatga ggtgcctttt atattgtgtg
    12841 ttttatcctc tacaccgaag accagatatc ccaaaggttg gttctgtata caagcactat
    12901 ttgataatgc tgatatacac atgccaattt cttcaattga atgaaagttc agtttgaatt
    12961 caacccattc actttcggtt gaagctttag tcaaatcgtt taaaagatct ttaagttctt
    13021 gatcacgcat attgcaaata atttagaatt aatttaactc accgctaaat gcctttttca
    13081 ataaactcct tttcaactct tctaaatcat tgattttggc ttgataaatg tcttccagtt
    13141 ttttggtttc agaggaaaag gcattaattt tttgaacgat gacttgttga tgtcttatag
    13201 gtggaatttt aatttttaaa ctttgcacat taactctggt aattcctgga attgcagccc
    13261 cgtttgataa agattggaag taataatatt tagtagaaag aaaagcatac aaaaatgata
    13321 cattaactgt ctttgctctt atcgccatta gctgtctgct tatacataac tcactttctg
    13381 acattaagtt aattttgcct acgcctgaac ctttaactgt aattagtata tcattcttga
    13441 ttgcggattg ttttggaaat tccgtccatt ttgaaactac aggatggatg actccgaaat
    13501 ctgacggacc agtaagatat ccaattcctt ttcctttaaa attgtattcc tttgaatcaa
    13561 tatgttgacc tgaaactaaa tcaatgactt cccccaaagt cttttcttcc catccctcac
    13621 cattattttc aaacactgac tgcaaataac tctcaaaaag ctctctggca ttttggaggt
    13681 tttgttcaga attggctttt gccctgtcta tggtggcaaa ggctgaatct aagatggatg
    13741 caatgcgttt ttgttcagag aggggtggaa ccgggatttc aattgcttca atttgagatt
    13801 tgttgatcga atcgaacact gcccccgcat ttcctactat ttcattttca tgttttagta
    13861 agaaatagaa gagaaactct ttgtcgatta attttgaagc tcttattgca gctaagcctc
    13921 tgccaataca tattttttga gtagagaaat taattggtcc aaccggagcc ctaacagaca
    13981 tcaatatgtc atttgcttcc gcttctttgg ttatttgcgt cgtccatgtt gttggttcac
    14041 caatatactt ttcaccaaac tctttctttc cttgatagaa gggtaaccct tttaactcat
    14101 tgttgtaata cttcccttca ggagattgtc cggcaattac ttcacaaacc tcacctactt
    14161 tttttatttg ccagttttgt ttcataaaat catcaaaata gaatttaaaa tgtctgcact
    14221 ttcactatcc agacttttca tttcttctaa tatttcatgt ggttttctta aaccagtttt
    14281 ctctttttta tttggattct taacactaag atcataagta aacttatcaa tagctttgat
    14341 atcaattgtc catgagtttt cactgttgga aagtgatttt tgaagcttta taaactcact
    14401 taagtcattt tcacttagag gattggtttt gccaagattg cgatctaggt ttaattgata
    14461 ataccatact ttcctggtag atgctccttt ttcgaagaat aggacaacag tttttactcc
    14521 tgcacctgta aatgtgcctc cggggagatc gagaatggta tgaagattgc aactttccaa
    14581 taataacttt ctcaggctga ctgatgcatt atcggtatta ctcagaaaag tgtttttgat
    14641 gactacccca gcttttcccc cggccttcag gatctttata aaatgctgca gaaagaggta
    14701 ggcagtttca ctggatttga tgggaaagtt ctgctgaact tctgcccgtt ctttccctcc
    14761 aaaaggaggg ttcgccaaaa cgatgtcaaa ccgatctttt tcctggatgt cagaaatgtt
    14821 ttcagccagg gtattggtat gtatgatatt gggtgcctct ataccatgaa ggatcatatt
    14881 catgatccca ataatatatg ctaaggattt cttttccttg ccgtaaaagg tttttttctg
    14941 tagcgtttca acttcattgg aagtaaggtt ttccggcttg cctttgctgt gcctgagata
    15001 ttcataagac tcgcataaga aaccagcact tccgacagct ccatcataga tcttgtcccc
    15061 aattttaggt gcaactactt taactattgt tttgatcaaa ggacgtggag tatagtattc
    15121 ccctccattt cgtccggcat tgcccatgtt tttgatctta tcctcataga ggtggctcat
    15181 ttcatgcttt tcgacatggg tgcggaacct aagttcatct atgcggttga taacttccct
    15241 aaggttatat ccactttgta ttttgttcct gagctcacta aaaatctccc cgatcttgta
    15301 ttcaatggta tcagcactgt cggcagaggt tttgaacctt ttaaagtaag ggaaaagctt
    15361 atggtcaaca aagtccttta ggtcgtcccc ggtcaaggtt ctgtgatgat tcagtttacc
    15421 cgtcttatcc tttggcattg cccatttgga ccattggtat tcgggagaaa cgatgttttt
    15481 ataaggcttc cctgtcagtt gggcagcagt ggctttatct ttctccaggt catccagata
    15541 ttttaggaat aagacccagg aggtctgctc tacatagtcc aattcggagc tgcatcctgc
    15601 gtccttatgg agtatatcat cgatattttt gaaagcttgt tcaaacatga agtattcttg
    15661 gttaaattga gttccgaaga tatgaaattt ggccgttgga cattttacta tccatccagg
    15721 taaatgagga taaaaacctg ccaaaatgaa ggtttttctg tcctaagaca ggcaaatcag
    15781 taaagtgtta ataaattctt aagaattcaa tcaaaccttt tatggcttca ttacagccat
    15841 tctgagcgat ctttattcca attggctggt tagcaccaat ccgtaggaga tcgtcgatcc
    15901 tgggctttcc tctgaattaa tatgaccaaa ttaactaaat tgtttaagta aatttttctc
    15961 ttgagagtcc atatctgaag attctttggt cctttcattt tttatggttg cgcttgataa
    16021 attagaacat gaatttggta cctcattcaa tactttagtc ctacttttat aattgcctgc
    16081 accaaaaatc ctgtcgagat gggtggtaac tatttccata acaatggaaa gatcctcttg
    16141 tctgcacaga attgcatcat gtttcggtaa aaccataaga ccacttctgt atagatcagg
    16201 caaaatgtga tctaagaata tggtactttc aatctcctga agtttaacgg ccagaaaatc
    16261 gttgcctgca ctcttggcat ctttattccc ctttctattg gaaaaagtat atttattcaa
    16321 ctcaggaaaa ctcccatttt tcagatggtc ttcaaaggtt tcaatcatat cgattttaac
    16381 cgaatcgagc aacttagtga gttttggaaa aactttggca aacttgagct ttcctacatt
    16441 tctgctttca ctttttgaat acaatgatga aataaagcta agctttgctt cttttcgtgt
    16501 tgtctcatta catgaaccga ataactgggt ggaaaagtat tcatacaatt gtccctcctc
    16561 agttttttcc ataaaaactt caatatctga cttagaagta ttatctgaag ggggtatatt
    16621 ggaggaaaac aataagggag gagaaagatg tttcttaatt gattgattta ggaacctatc
    16681 cgcagagata ggttccatat tattatttat tatactttta attaaataag ataaaattaa
    16741 tggttgacta caactaagat caatttcaca taaacaatta ttatctaaat ctaataattt
    16801 tagcaattca gaaggtaaat ttgtaagatt gtaatttatt cttccatttg ttgttgattt
    16861 acttatataa aagtgtttta agttaagtag ttttaaacca cctttgtagc tttccgatat
    16921 atttattttc tcaaatgaca agtagggctt aatgtattca gaacttaagc tctctttata
    16981 tgaaattttt gcatatttgt acttaattcc tttcattgta attttaaatt tattatcaat
    17041 ataatcttga cttaaagaat cacttttatt gttaatctgc ccatcggaag tcgaaacatt
    17101 aacatatttt aatgtatttt caatcaacct acacatgttt tcatcgtcaa ccttgaaatc
    17161 attcagattt ctggcatgtt caagatacat aacatcacca tctaaccatt tcggagaaat
    17221 gcggtaagat ttgcatatat gtccagaata actgtaagct ctatttccgg gcaatacttt
    17281 cggttctaaa atttctaatt tttccaattc attccaaata ggatagaact cacttccacc
    17341 caaaagcttt ttcttggttt tataatcata aggtaaataa tgtaacatgg gtgcaaagca
    17401 tttttgatgt tggaaaatca tttcctttat aatggataat gcttttacac ctttggttgt
    17461 attaccaaat acggtggtaa ttatattaca aatcctcttg gggactggca caatgaccgt
    17521 catttttttg ctactactca tcgcatttat tttaaagtga ataagtagtg tttggtcgat
    17581 tgtactttcg gaaagtgttg atgtttgcat caatttctga cttgtcaaat cgtggtgatc
    17641 cgctcaaatc aattttattg agctttccga tcctcgccca ataatcaaca gtacttactg
    17701 caataccaag gtaggttgct acttgagatc gggttaggta ccttgaatgg tccgggagac
    17761 tctttgtgga aaataaacta gaaccctttt ctgcaaagaa ttctttaata gctgccttga
    17821 cttcagtttt taagaaatcg gcaaactggc ctgaattgta aaacatagac attgttttga
    17881 aagacctttt gatatccgcc cacactccat tttactggaa ttggcttctc caccgtaaca
    17941 caatgtgaac ggttggcaac tcaaagattt gagtactcta tagataccta agctcatgat
    18001 taaaaaataa aattatttat gccctttagg cgttgaaatt aacgtgatta attgagggtg
    18061 ggttcctcct aaattgttaa ggaaataata aaatattgaa acaaattggc ttagttagaa
    18121 atagcattga aaaaactgtg ccgggccatc aataaagcat tttcttcatt atcgaattta
    18181 atatacttca ggaagatttc tggtgtctta tgccccgtaa tcctcataat gctaagcatg
    18241 ggtacaccag ccttgtaagc gttcgtggca aaggatcttc ttgccgtatg ggatgctacc
    18301 atctcatatt ttttgtatga agcttcatac attttccctc ccctatattc tctctttatg
    18361 attatgtcat ctaactctgc cattttgcac aattctttta agtaatcgtt cattttctga
    18421 ttgctgatag gtcttggtag cttattacta tatttgagca gtataggttc gacaattggg
    18481 tgaagcggaa taattaaccg atcccctgtt ttcgtaacgg tggtatcaaa aactttaact
    18541 ccatcgagaa acctcaagtt ctgtggttgg agaatggagt aatctgagta ccggagacca
    18601 gtataggagc cgacaataaa caggtcacgt attttatcaa gtcttgggtt acctgataag
    18661 tccagaagta tcaatttttt gagatcggat tctgtgaaat atatattatc ggtctcggtt
    18721 ttggaaacgg ttatttttct gctcttataa tccatatttt tattaaatcc tctatctgtt
    18781 gcatcattga gcattgttct aatggttgtt attatcttat tgatatgatt gttggagtaa
    18841 tctttgtcgt agagatgttg aataaatcct aagagaaatt caatactgag atcctcaaaa
    18901 taaaccactt tacctttggc atatctattg aagtggtttt ttgttgtttg gtaaaccgct
    18961 attgatttag gtttatattt tggagatcta ctgcgttctg caataaactt atccataaaa
    19021 gaactgaaat ctttaacccc tgaatgtctg attttcccat gatataaagc atccatttcc
    19081 cctttgaagg caagtgaagt caggatcttg ctttgtgaga tgtattgctt ggctattgat
    19141 agagccaggg attctaattt atccaattca tggtttatga attggtaatc aataaaatcc
    19201 tgggttgcct tggcccggtg agtattatca ttccaatatt tagggggaat ttttaatccg
    19261 gttgaataga caagacgctg cttcaacgga cgaaaaatca ttgagattag ggtctctttt
    19321 tcggatttat tctttaaatt aaactttggt ctgttcattg agtaaaaact ttagttgtcg
    19381 aaatagttgt cgtaatttat gcactaaatt acaagaattt agtcataaaa attgcataaa
    19441 gcgttgatat taagcgatat tagtattaat taagaataaa ggttctggtt ccttattctc
    19501 cacggaatca tagggttgtg taaactttac ataacccttt tttatttccg gccatttgca
    19561 gtcaaatctc attcccttaa taccctattg ggtcaagacc tggctccggc ctgtaacaat
    19621 taatttttaa gttcatcctg aatttcctat cttcagagag aattatagtc ctttttcccg
    19681 cttttctccc agcttcggtt tcggtcaggg agtgagtcaa gcaggctcga tccattgaaa
    19741 acatttgccg gttatttctc aggaatatgc cttgagccct aaatcaaaac cgggacatcc
    19801 atgaagcgcc cgtttatcca aaaaaccttt taaatgtttt caaaatgaat cagaaaaatg
    19861 tatttaccgc actggccatt ttattgatgg tacaggcact ggccttttat tttatgggcg
    19921 gaaaaatagc tatggatgcc tttggggatc tgggcgaacc gggcaatatg gcagccaaac
    19981 acctcatgga gataatggcc gccttcgctt ttaatttcgg actgcttgct tatgccaccc
    20041 ggtctaattc gcaggtattg tgggtcttta cccttggctt tgccattgtt gtggtcgtca
    20101 cccttaaaca catcctggca gacggcatac atgtccccat ttgggctatc gctttccaga
    20161 tcctcagcct tctggccgtc ggttatctgt ggatgaggca caacaaggtc aaaccggctt
    20221 aagaaccgat aggtttagat tgtctgttac cagccggctg ttgaagggcc cagtttgacc
    20281 cggatccggc ggtttccaaa tcaaacatcc gctaaccgga cagaatgaat aattttgtcc
    20341 ggttaaattt gattcctctt tttgaatcct aacacggcgc catgtttcca ttccaattcc
    20401 tgctgatcct tttattgacc ggggctgaac gctccgacgc gaccaatgac caattcctcc
    20461 tttttgatgt cgagtttacc tataccaagg aagaagcgga ccattccaaa ccgagcaaat
    20521 cccattatta cgttaccggc gatatgctca atacccaaag gcccgcggac tggacctcgc
    20581 cggtggacta ccggaatggc agcgtgcaca tccggctgga agtgatcgaa aagccccagg
    20641 gaggggcgcc caccacctgg actctttgtt atatccccaa tcaaggacag ggcaatggat
    20701 acggctgcac cggcaccgga ttgtatacgg ataaaggcat ctatgaaaaa gatatcgcca
    20761 tgacttcttt ctgggaaaat gaatccatca tctggtccaa gggcatcaaa caaatggacc
    20821 tggtgatcaa ggatgacagc ggaggtcagg gacatgccca caaaagacag gaccatgagc
    20881 ggtttttccc gacccgggtg cgcatcacca tggttcaggt ggcggcggga tcgacctatg
    20941 acccgggcat tttgccgaaa acagccttgt accgggagga ccgttaagac gaagcagcat
    21001 gttcggacga agctttgctt cggcctggta ggattaatca tgaattacaa tttttcatgg
    21061 ggtcagagcc gttcggatga agcttagctt cggcttccac acacctcgag atgtaactta
    21121 gccacggttt accctatctg aattacggaa gccgcacctt gcaagcaccc caccaatacc
    21181 cttaatttta cccgcctgat gtataaagcc tccagactgc tcttccgcct atttctcggc
    21241 ctctttgtgc tttacctgat cggatttggg atcatctatt ccatgcagga ccgctttatt
    21301 ttccagcgcc aggtattgga ccccaatcac cggtttgggc atgccgcaca agagctttat
    21361 attccggccg ggcaggatat ccgcctcaac gcccttttat ttaaaccgga agggaccccc
    21421 aaaggactgg tcctgtattt tcatggcaac cggcattcgc tggaccggtg gggacgtttt
    21481 gttcaattcc tcatcaactt tggttatgag gtccttattt tcgactaccg gggatatggt
    21541 aaaagcaatg gagaaccttc agaagagggg ctttatgaag atgccgaagc ggtttatgac
    21601 tacgcacgga cccattttcc cgaaaagcct ctgatctatt acggtcgttc tctcggtacc
    21661 ggggtggcca ctcacctggc tgctaaacac gctcctcaaa aactggtgct tgaaacccct
    21721 ttcgatgaac tgaaaaacgt ggtcccggcc tatttccgtt ttatgctgca tatcattccg
    21781 cttcgtcatg ccttccggaa caacgaaagg cttgccagca tcacctgtcc catcctgatc
    21841 ctgcacggca cccaggatcc ggtagtgccc ctgtcttcgg ccctcgggct caaaccccta
    21901 ctgaaacctg gggaccagtt ccgcatcgta cccgggggaa ggcataacaa cctcacccgg
    21961 ctggatgagg gaatcgatgc actaaaatcc tttttaaatt aacgggtatg cctatcttca
    22021 aacctgattc taaaaccgta tgtatgaaaa ataaaatctt tttcgctctt gggttgcttt
    22081 taagcgcggc cctcctgcaa gcccagcacc tgggcgcccc gccggaatac gtcaaagcgc
    22141 tgacccctct ttggaaaggg gaaaggtttg cagacggaag acccaaggta cccgatattg
    22201 tattggagag cctggagtcg gccactctgg aacagatctg gggtttcctg ggccggaaag
    22261 gatttcgcaa ccaggtcgaa aaggactgga tcatccttaa acccggggag actatggtcg
    22321 gccgggtagt caccgctcag tttatgccct caaggcctga tctcgatacg ttggtccgct
    22381 cccttggaaa agcggaaggc cggtcgcaga agggcggcat taacatctgg cccatcgaca
    22441 tcctgaccaa aggggatatc tatgtggccg atggtttcgg aaagatcaaa gacggaaccc
    22501 tcattggctc cagcctgggc aatgccatcg tcggcaaaac cggcaaaggc gtgatttttt
    22561 acgggtccgt ccgggatatg caggaattaa aagataccaa aggattcaat gcctgggtga
    22621 aggggcatga tccttctttc atccgggaca tgacgcccac ctccatcaat gcacctatcc
    22681 gtatcggcca ggtgaccgta tttccgggtg atgtggtttt tgcgaatgaa tacggggtgg
    22741 tctttattcc cgcctacctc gtggaagaat tggtttcctc ttccaccctg gtgggcctgc
    22801 gcgacgagtt tgaacgcttg ctgctccagc agggcaaata cccttcgggg cagatccacg
    22861 gcgactggag cgaggagatc aaaaatgaat tcagggcctg gtttgcaaaa tacccgaaac
    22921 aactcccggt cacaaaagaa gacatcgaag cctacctgaa acggggagaa taacggcccg
    22981 ggtaatattg ttaaaccaag accacaattt ctatttttta atatcaacca catgaaattc
    23041 agtctcacca ttttgttttg ctccttttgt ctattaacat ggaatgccac cgaggctcaa
    23101 accatttcca gggaagaact catctttctc acttccgagt ggcagggaga acggtttgcg
    23161 gacggaaggc ccaaaatacc cgacgatctc ctggaccggg ccaggcgcat caacatcgac
    23221 gatgcctgga cggtattaaa aaactacggt tatgtcaatc aatttgaagg cggctggaaa
    23281 atggtcaaag aagacctgcc ggtgatcggg cgtgccatga cggccatgtt catgcccagc
    23341 cgtccggatg tggagagaaa aatcaaggaa cgcggaacct cccgcgacgg aaggaaggga
    23401 aataccaatt cctggcctat tgaaaccctg gtaaagggcg atgtctatgt ggcggacgga
    23461 tttggaaaaa tcggcggcgg gaccctgatg ggttccaccc tggctacctc catttacagc
    23521 aagtcgggca acggcgtggt cttcaacggg gcatcccggg acctccagga aatctcgaat
    23581 atggaaaact tcaatgcctt tgtacgggat ttccatccct cctttcttga agagatggtc
    23641 ctgatgggac tcaacacccc ggtccggatc ggtggggtga tggtccttcc cggtgatctc
    23701 gtcattgcgc gcaggacggg ggttcttttt gtgcccgctc atatggccga agaagtggtc
    23761 tccacggcgg aatttgtgat ccgcaaagac cagtttggtt atgaaatggt ccggaccgga
    23821 aaatattcca cgggccagat cgacagccag tggacggaag aattaaaggc ggaatttctg
    23881 aagtggctgg aaaagcgccc ggacctgggt aagatgacca aggcggagct ggatcagagg
    23941 atgagtaaaa ggacctggta gccatcgggt aattggtatt ccggggcttg ctactaattg
    24001 gatttctgac actgtgagca ccttttataa gccatttcta attattgagt ccagaccacg
    24061 gaattggatc gaatcaaccg gacaggagga tcttatattg tgattcattt tttagttagt
    24121 caaacaatat tttaccttca atttaatcgt cgccatgaaa aaagcaattc atcaactcat
    24181 ccaatccaat aaaaaccagg aggcagaaag ggccaaagcc atcaccggag aaatcaataa
    24241 cccggccacc ctgcccgccc ggcgcgattt tctgaaaaag accgccctgg gaggcattgc
    24301 cctgaccggg ctgggccatc tctccgcggc agaaaccatc gaaaaggcaa gttccaaagt
    24361 cagccggatg agcgcgccga ccggcctgaa gatcaccgat atgaggtata ccaccatcag
    24421 caacgggacc ggggccacca atgcgcgcaa tgtcatcctc cgcatcgata ccaaccaggg
    24481 catatacggc ctgggtgaag tgagggatgg cggggatgcg cgttttgccc tttacctgaa
    24541 aagccggatc ctgggcctca acccctgtaa cgtggagatg attttcaaga tcatcaaaca
    24601 attcggaggg catggccgga tgggcggagg ggtcagcgga gtggaaatgg ccctctggga
    24661 cctggccggc aaagcttatg gggtgcccgt ctgggcttta ttgggaggcc gttaccggga
    24721 taagatccgc ttgtattcct atatcccgaa ttccgggggc cggcccctgg ccgaaatgga
    24781 cctcgaacaa tttaaagcgg atgtcaaacg gaggatggaa gtccagggtt tcacctggct
    24841 caagatgcac ccgggaattg agaaggtggc caagatcccg ggcactacgg taaataccaa
    24901 attcatcccc ggattcaacg accgggtttc ctcttcccgc tcccaaagct acctgcacta
    24961 ccagaataca cggcatgcct ttacttcggt ccagcttacg gataaaggac tggaggaact
    25021 ggccagatat gtggaaacca tccgcaacgc ggtgggttat gaggtgccct tgtccgccga
    25081 ccaccagggg catttcgata tcaatacctg catccgggtg ggccatgcgc tggagcgctt
    25141 caacctggcc tccctggagg actttttacc ctgggatcag acggaacaat taaaaaagat
    25201 caccgatacg atcaatacgc ccaccatgac cggggaggac atttatctca aggaagcctt
    25261 taataattta tgcgatcacc atgccgtgga catcatccag ccggacatgg gttcttccgg
    25321 aggtatcctg gagaccaaga agatcgggga ttacgcggaa gaaaaagata cgcccatggt
    25381 gcagcatttt gccggtaccc cggtctgttt tatggccaat gtacattgcg ccgcggctac
    25441 tcaaaatgcc ctggccctgg aagtgcccaa ccagattgtg gacaacccct ggtggcccaa
    25501 cctagtgaaa atggtgggga aacaacctct gtatgaaaag ggttacgcca acgttccgga
    25561 cgacgcgccc ggcctgggtg ttgaattgaa tgaggaagaa atgaagaaac atttgcatcc
    25621 ggaagatccc agctattttg tgcccactcc tcaatggaat gaggtcaggt cttacgacag
    25681 gctgtggagt taatcagggt gtaaacgaaa gagtgcggcc aaatgggctg gtaaaaaatt
    25741 atgaaatcat atcttgcttt attcatggtc ttgttggccg tccgggtagg agcccagggc
    25801 cagtttccat cggcatccat ttccaatggc ttgatcaaag ccggctttta tcttccggat
    25861 aaagacaagg ggtattaccg cggttcccga tttgattggt ccggggtcat gccttccctg
    25921 gaatttcagg ggcatcagta tttcgggcaa tggttcgagc gttacagccc gaccctgcac
    25981 gatgccatca tggggccggt ggaagcgttt tatcccattg actttgaaac cgccaacccc
    26041 ggtgaagact tcctgatact tgggatcggg gtcttgaccc ggccggatga taaaaagtat
    26101 tctattgcca cgacttatcc cattaaagat ccgggcaaat ggaaggttcg gaaaaaaacg
    26161 gaccaggtgg ttttccggca taagctgcat ggcctgggtt atgcctatga ttatcaaaaa
    26221 acggtaaggc tggaaaaagg aaaacccgaa ctggtgcttt cgcattccct gaaaaatacc
    26281 ggcaacaggg ctatagagac caatgtgtac aatcataatt tttttgtgat ggacagccag
    26341 gtgacaggcc ccgactttgt ggtgcaattc ccttttaaga tcaccgggga ggttcagaga
    26401 gggaacgaac tgggaggtat ccgggaaaat gaaattgttt tttataaatc tattgaaaaa
    26461 aacaaaagcc tctattttgg ggcccttgaa gggtttgggc aggatccccg ggactatgat
    26521 atccgtattg aaaaccataa aaccggggcc gccgtccgga tcacctctga tcagcccctg
    26581 atgaaactgg ccttctgggc cacccaaatg accttatgtc cagaaccttt cacccggatc
    26641 agtgtgaaac cgggagaaga gttccaatgg gaggtgcgat atgaatttta tacctgtgcc
    26701 atcagggatt aaggaaaaat gatgcccttt cagggcggat atggcatatg accccgcagc
    26761 catgggcgtt gcgcccttgg ctgcgggaat agagcccttt cagggcgatg agaacggatg
    26821 ggccccaaca atgctcatct cacccaatgt tggtggaatg aagccctttc agagttggcc
    26881 tatttagaga gggcttctat aattttagtc aagaacggat tgtttcctgt gatttaatcc
    26941 tagtcaggca gcaccatcgg cctgaaaggc cctcattctc tagcattggg cgatctcgcc
    27001 caatgctaga gaatcaatga cgcaattcgc cctgaaaggg catcatttag ttttgacatg
    27061 gatattattt ttgtctccta aacacctgcc ccatgggaca atccctcgtg aaaatttata
    27121 cccatatcgt attttctacc aaacatcgtc aggcattcat aacggacaaa attgagcagg
    27181 aattattcag ttatatcgga gggatctgta atcggctcgg ttgccagaca ataacggtag
    27241 gaggttatag ggaccatgtt catatccttt gcttgttgtc aaagcagata acgatcatga
    27301 aactcatgga ggaggtcaaa gggcattcat caaaatggat taagactgta ggtcaggaat
    27361 tccggaattt ttattggcaa aacgggtatg gcgccttttc ggtttattcc aaggatgtgg
    27421 accgcgtggt gcaatacatc cgttcacaac gggaacatca ttccgggcgt tcctggcagg
    27481 atgaatatcg cgatatatta atagaacatg agatggactt taatgaaaaa tttgtttggg
    27541 attgaatgta tgatgcaatt agaataatgc ccttgcaggg cgggtttagc acatagcccc
    27601 gcagccttgg gcatttcgcc caagcctgcg ggaatagggc cctttcaggg cgggttcaac
    27661 gcgcggcctc gcagcattgg gcctcccgcc caatgctgcg gggaatgtag cccctacagg
    27721 gcgggaatat gtgaaaaaac cagatgaatg agagttgact acccgattgc aaatcagcat
    27781 gcgtttgtct accaggggct tcccaccgtg ttctatagtt attccatccg aattgtgctt
    27841 ggcggggtac tgggggtccg tcatcctggc aagtttcgcg aagatattgg gagcgtgcgg
    27901 agatattcat tcggcctgaa aggccctcat ttccctagca aggggcggac ccgcccattg
    27961 ctagggaaat caacatgacc ccattcgccc tgaaagggca tcatttgttt cactaatgtt
    28021 cacatccccg aaatcctgag tattttcgaa tcgatttccc aagaaaataa ggcatgaaaa
    28081 tcattcatta catcacgcta tctggaatcc tgtggtttgc cgcttgtaaa aagcccgatt
    28141 catatgcaaa ccttttagca gatggaaccg gcaaggacta ccccgcttat gggggtaaca
    28201 aagccaatac ccggtattcc tccctggacc agatcactcc ggacaacgtg aaccaactca
    28261 aaatcgcgtg gtcctatttc gccaacaacc caccggacac cgggcggccc cgttccggcg
    28321 gcgagatcca atgccagccc atcgtggtcc atggcgtgct ctacggcacc tccgcggaat
    28381 taaacctgtt tgccctggac gcggccacag gaaccgaact ctggaaattc acgcctcccc
    28441 aggaaaacct cagacgccat gccagccggg gagtcatgca ttgggaatcg ggagaagacc
    28501 agaggattta ttacaccgcg ggatatcatc tttattgcat tgatgccgga tcaggcaaaa
    28561 tcataaactc tttcggggat agcggccgcg tagacctgca tgcgggattg ggtgaaaacc
    28621 tggatcatga agtcaataag ctccatgtca ctgccacctc cccggggatt atttttaaaa
    28681 ataccctggt catcggttca agcgtttccg aagggggcga tgcggctccg ggccatatcc
    28741 gtgcctttga cgtgaactca ggcaaactca aatgggtctt ccgcaccatc ccgcaacccg
    28801 gcgatcccgg gtatgaaacc tggcctcccg atgcctggaa aaaattcggg ggagtcaata
    28861 actggagcgg gatgtctttg gatgaaaaga gaggcctggt ttatttcggc acgggatccc
    28921 cttcatcgga ctattacggc ggggaccggg aaggggccaa cttatacgga aactgtgtca
    28981 tcgctctcga agcggaaacc ggtaaactca aatggtattt ccaggccatc cggcatgatc
    29041 tctgggaccg ggattttccc tgcccgccaa acctggctac catcacccaa aacggaaaaa
    29101 agcgggaggt ggtggttcag gcaggcaagg atggttatat ctatgtgctg gaccgggata
    29161 ccggggagtc ggtctttccc ttggaagaac acccggtacc ggtggagggc ctgcccgggg
    29221 aacatcctta ccccagccaa atcactccgg ccaaacctcg gccgctgatt cgccaattgt
    29281 ttacggaggc cgacattacg gacatatccc ccgaatccca tgcctttgtc aaaaaacggc
    29341 tgcaggaatt tccacctacg accaacaggc atcaacctcc cagagaggcg ggtaccatcc
    29401 tgtttggata cagcgggggc gcagaatggg gtggaggggc cgtggacccc gagggcatcc
    29461 tttatcaaaa cgccaatgaa gctccctggg aactcaccat gatcgataag gcctcccggg
    29521 ataaagaaac cgccaccctt tcccccggcc attcattata tctccggaat tgtgccgcct
    29581 gccacggagc cgaccgcagg gggaacagca acctggtgcc gaccctcgtg gatatcggca
    29641 agaaacgatc cgcagaggat atcgcctcct ttatccagac cggcaatgcc cggatgcccg
    29701 catttattta tttatcggaa aaggaaagga aagcccttgc agcttatttg ttggaaaccg
    29761 aaggctccgt gatgcctgct ccaagaactg ctgaagtcat gaaggaaaag aatagaccag
    29821 actttcctta cgagcccccc tatatgatca aggtttggca acgcttcgtg gatcaaaacg
    29881 ggtataatgc ggtcaacccc ccctggggca ccttaaatgc catcgacctc aatacgggtg
    29941 aatacctctg gcgcgtgcct ttgggtgagt ttcccgaact gactcaaaga gggatacccg
    30001 tcaccgggac cgaaacttat ggcggtccgg tggtcacggc gggtggcctg gtcttcatcg
    30061 cctccacccg ggatgaacga ataagggcct ttgacaaaag gacaggtaaa gtcttatggg
    30121 aatatcagtt acctgcaggc ggattcgcga cacccattac ctacgaagta tcgggcaaac
    30181 agtatatcgc cctggccgta ggcgggggac gaggggcgaa accgggcgga tggtatatgg
    30241 cgttttcatt gtaaaatagg ggatcaccgg ttggctttta tcataccagg gcgtattgat
    30301 atggcaggaa ttttgtttaa ttttagattc ctttttttcc tttacttatg aaagggtctt
    30361 tatacggcct ggtattttta accatcctgc tcgcagaagt agggatcctg tcttgccgga
    30421 acggcgggag cggaatggaa tccaggctcg aaagaagttt ggccaaggca ggtagtaacc
    30481 ggcccgaatt ggaaaaagta ttggagcact atgccttcgc cccttccgac agcctgaaat
    30541 acaaagctgc aatctttctg ctggcaaata tggaagacct gattcatttc cggagtaaat
    30601 ggcaggagca gtttgatcct gtctttttta aagcgatggc cggaaagacg gttgaggaag
    30661 tgggtccctt aaggaagtcc gtggattcat taattggcaa acccgactgg aaatcctttg
    30721 agaaaattcc tgatttgcgg accttgacct ccgaatacct gatccagaac atagatcagg
    30781 cctttgaagc atggcgatcg gccccttggt ccggtgatgt aagttttgat gctttttgta
    30841 attacatcct gccttataaa aaccacaacg aatatccgga agtatggcgt aaaacgcttc
    30901 gcgaccgtta tggtcacttg cttaccgatt cggtggttcc caaaacgatg acggatatct
    30961 gctgcgccct ggtggatgaa caaagatggt ttgactggac cgatgactat ggattctatc
    31021 cttccgtatt tggtttcagc caactgatgg aaggccgcaa agggtcctgt accgaaatgg
    31081 cccacctggg agcctatacg gcaagggcat tgggtattcc ggttgccgtg gattatattc
    31141 cgcagtatgg caatatgcac gggtcccatg tatggaacgc cttgatcacg tcggattcga
    31201 gctaccttta ttttgaaggg gcgggcggca ccaggcccgg cgacatgttt tatacccgcg
    31261 aaaaagattt caggctggcc aaggtattcc ggaggcattt tgacctggta cagggaagtt
    31321 atgccgcccg tgccatggaa gccggcttga aggacattcc ggaattgctg caaaatccaa
    31381 ggatcatcga tgtcacggga ggttataccc aagtggccaa tctcagaatc catggactgg
    31441 gccgggaggg ggaacccgtg ttcctctgta tttacaaatg gcggaactgg accgcggtcg
    31501 ccggagactg ggttaaggat ggacaggctt ccttcccgca gatgggcagg ggactcgtgt
    31561 acatgcccat gtattaccgg ctcggcgaat atgaaccggc cgggccaccc tttgtgttgc
    31621 cggtgcatgg aaatatctgg tacccggagg cagatgaaaa aaacaggcaa tccgtgcttc
    31681 tgaaaagagt ggatctcttc aagcgcaatt gggaatataa tctcgccaat caaatgaccg
    31741 gggcccgcct ggaagggtct gcggattcaa acttcagtca gccggtggta ttacatcaaa
    31801 tccaggcgcc ggtaaagatt taccggggag ctctggtgaa tgatctcgat cagaaaggga
    31861 gagcaagtta cgattccacc tggaagaccg taaccttgac ccggcctgaa aaattcaggt
    31921 acgttcgcct gatctttgat gaacccttgc tgttccgtct gggagaactt gaattttatg
    31981 attcagagag cggtccacca ctgacaggta tacctgccgg aaatgtgcct gaaccttcca
    32041 gggcctttga cggcttcccg ggcaagacca ttaaaatgac ggaggagacc aaccggtctc
    32101 attgggtaag tctggacctt ggctcaccca aatctatccg caagatccgt tatttgccgg
    32161 ctcatgatgc ccggaccgtc caggaaggca aatcttacga attgttttac tggaaaggga
    32221 gatgggaatc cctgggcagt caaaaatgca acggattgcc tctggaattt caggaggttc
    32281 cttccaacgg attgtactgg ctgcactgcc gggcctgcga cgacaaagaa gaacgcatct
    32341 ttacctacga agacggtaaa cagatttggt ggtaaaacgg gcgcctcttt atccggattg
    32401 gtggtccaga tcagaagagg tttttgatta agcctgacca cgagggcctg ccggcaatct
    32461 ttacttttat tgtaaaatgt attttggatt acggaaccgt ctttttcact acttttcctt
    32521 ttcagaatta atcatccatc atgtcagcca gtaccatcat ccctccggat ttgaatatca
    32581 aacaatggtc gaaaaacatc aagggcgagg tgctgaccga ccggtattcc ctggggatgt
    32641 atgccacgga tgccagtttt taccagatct ggccggtggc cgtcgtcctt cccagggacg
    32701 aagaggatgt caaaaaggcc gtggctttcg ccgggcagca caaactgaaa atcctgcccc
    32761 ggggaggtgg gaccagcctg gccggccaga cggtcggcga cgcgctcgtc ctcgattttt
    32821 ccaagtatat gaaccaggtc ctggaattca atgaacagga aaaatgggtg cgtgtacagc
    32881 ccggcctggt ccgggatgaa ctcaatgaat acctggccaa atacaaactg cattttgccc
    32941 cggatcccgc tacagccagc agggccaatg taggcggcat ggtgggcaat aattcatccg
    33001 gcaccaaaag catcctctac ggcaaaaccg tggaccatgt gctggaggca agggtattgc
    33061 tcgccgacgg gacggtacta aaactcaggg aactcgaccc ggaagagtac cgtcgcaaaa
    33121 cgagccagca ctgccgggaa gggcagattt accggggttt ccaggaaatt atagagaaaa
    33181 acagggaaga aattaagaac cggtttccca aggtaatgcg gcgggtaggc ggatacaacc
    33241 tcgatgaatt cgtttatacc gatcggtgga acctggctaa actcatcacc ggcagcgaag
    33301 gcaccctggc ggtcaccctc gaactgaaat taaacctggt ccccctgccg aaattcaaat
    33361 cggtggtcgt cgcccatttt gcggacctgc tggaatccat acgggcggtg gagcccatgt
    33421 tgccttacca gccctctgcc attgagatcc tggaccggac cgtcctccat ctcagtgcgg
    33481 aaaacctgac cacccggcat ctttgttacc tgatcgaagg cgatcccgca gccatccaga
    33541 tcgtcgagtt ctacggggat acccgggaat cggtcatgca aagggccagg gatatgatcg
    33601 cagacctgca tcagcgcggt ttgggttatg cctttccttt atttcccgaa ggcccggctt
    33661 ttgaggatgt atggacctta cgtaaaaaag ggcttggcct gatgatgggt ataaaaagtg
    33721 agaaaaaagc cctgagtttt atagaggatg ccgccattcc cacccaggtg cttccggaat
    33781 acatcgacca ggtgctgaag atctgtgcca aataccatac ggaggtgtcc atgtatgccc
    33841 atgccagcgt gggcgtcatc catgtgcagc ccctgctcga cctccgccag gcggaggata
    33901 ttgaaaacct gaaacacatc acggacgaga ccttcgaact ggtcgtcaaa tacggaggtt
    33961 cctggagcgg ggaacatggc gacggcctgg tcagaagcgc ctacaatgag cggtttttcg
    34021 gaaggacctt atatgaagct tttcgcagca taaaaaaatt attcgaccct gaaaaccgga
    34081 tgaacccggg aaaaatcgtg gactccacga ctattgagca ccatttgcgt tacggtacag
    34141 catataggga ccgggaagtt aaaaccgttt atcactaccg gcaggaaaac agtttccggg
    34201 aatcggttca tatgtgcacc ggggtggggg aatgccgcaa aatgctgggg ggtaccatgt
    34261 gcccgagttt taaagccacg cgggatgaag aacattcgac ccgtggcaga gccaatgcct
    34321 tgagattggc catgtcgggc caattggacg aggagggcat cggcagtgaa cggttgcacc
    34381 aggtcatgga cctttgcctg tcctgcaagg cctgcaaatc ggaatgcccg agcaatgtgg
    34441 atatggcgaa attgaaaggg gacgtcaccc agatctacta cgatcaacac ggcactacct
    34501 ggagggacag gcttatccgc gactcctcct ccatggcagt ccggctggca ggatggccgg
    34561 cgcgggtagt caatgggata caaggttctg ctttgttcag gtatgttatg gataaatgta
    34621 cggggatcga ttccaggaga atccttcctg gatatgcaac ggagcctttt tataaatggt
    34681 ttgaaaagaa taagaataaa gtcaatcccc cggctcaaaa aaaggtggta ctgtttgcgg
    34741 atacttattt gaattttcat gagccccaca ttggcgtatc cgccttggaa ttgctcaatt
    34801 cctgcggtta tgaagtgatc ctggccaatg tgggttgctg tcagcggccg aaaatttccc
    34861 atggttttct tcgcgatgca aaggccgcgg gaacggctac actggaaaaa ctcaggcctt
    34921 atatggagca gggattaaag atcatagttt gcgaacccag ttgcgcctcc gctctcaacg
    34981 atgatctgcc cgacctggtg gaagatgaaa aactggccca attggccaag gaccatatcc
    35041 tgatgatcga tcagtttctt tccagggaac tggaaagcgg aaatatcaat aagaagttta
    35101 aatccattgc cggggatatc ctcatccacg ggcattgtca tcaaaaagcg ctttacggca
    35161 ccagggcaat gaaagaggtt ttcgggcaga ccgggaattc cctcagcgaa attccgtcgg
    35221 gctgttgcgg catggccggt tccttcggat atgaaaagga gcactacgac ctttcccgga
    35281 aaatcgggga tcaaatcctc tttccagcgg tcaataaccg aaaaccgggt acgaccctgg
    35341 tcgccaatgg cttcagctgc cgtcaccaga tcgaacattt taccggggtc aaagccaaac
    35401 actgggtgga ggtgataaga ggtgtgtaag caggtggaag ccaccttgca ggcgccgaag
    35461 taagtagaaa acccggtggc tttcaccttg ggaatgaagc gggcggagcc accttgcagg
    35521 cgcctcagta agtaaaaaac ccggtggctt tcacctggag aatgcaagtg cagaaacggg
    35581 tgaaagccac tttagacctt tcctggggaa tgcaaagcgg gcggaagcca cctaacgacc
    35641 ttggaataaa acggggtgga agacaccttg gaatcaagct tggaagcaag tggcagaaac
    35701 cttcaaattc aaactccaag tagatatttt gcccgctcaa tattgcaaag aggtttctcc
    35761 tctaccttcg aaaactcacg ggccgatgaa tatacccact cctcggcagt cctaactaga
    35821 cgggccttta ccggattttc atggatataa ttgaaaaccc tcaaagcata aacattttct
    35881 acatggctga gtgctttacc ttcgaactca gcgatggacc gaaaatcatt gtcattattc
    35941 caaccatctt tttttttcgt ttttgacctg aatagagacc ctgtccggtt aaaccgtcga
    36001 ttaatccctt tagtatatga actcaataac tggctaatgg caaaggagat gttttgaagc
    36061 ccggaatttt tatactcttg cacggataaa tccgaagtat aaatcaatag atggaaatga
    36121 ttcggcatca aacaataagc caggaggtca gcgtttggac atacatatct cctcaatttt
    36181 gctttaaagt attcataatt acatggttcg aagaatattt tttcccggtt attcccttgg
    36241 ttaaaaatat gataaacctg atgcgactgg tatctcattc tagtgtagtg tgtaatccca
    36301 aatttatgca aattgagtgg aagtccggtg tctttcacct cgggaagcca tttcgcagca
    36361 ggtggaagac accttggatt cgccgcttgg ctacgcaaag tgattgccac atgcttcgcc
    36421 cgaggcttgc gctaagtgac aaccacttgc gccgctcggt gtctttcacc tagggatggc
    36481 cagtgcgcag caggtggaag acacctttgg atcacatctg gggatggcaa gtacggaaac
    36541 gagtggaagt ccggtgtctt tcacttgggg gagccattgc gcagctggtg gaagacacct
    36601 tggattcgcc gcgtggctac gcaaagtgat tgccacctgc ggtgcttagc actggcgcta
    36661 cgtggcaacc acttacgccg ctcggtgtct ttcaccaagg gaagcaagtg cggaaacgag
    36721 tggaagtccg gtgtctttca cctaaggaag ccagtgcgca gccggttcgc cgcgtggcta
    36781 cgcaaagtga ttgccacatg cttcgcccga ggcttgagct acgtggcaac cacttacgcc
    36841 gctcggtggc tttcacctag ggatggcaag tgcgcagcag gtggaagaca ccttggattc
    36901 gccgcttggc tacgcaaagt gattgccaca tgcttcgccc gaggcttgcg ctaagtggca
    36961 accacttgcg ccgctcgttg gctttcactt gggggagcca ttgcgcagca ggtggaagac
    37021 accttggatt cgccgcgtgg ctacgcaaag tgattgccac atgcttcgcc cgaggcttgc
    37081 gctaagtgac aaccacttgc gccgctcggt gtctttcacc tcgggatggc tggtgcggaa
    37141 gcagcgtgga gttctggtgg ctttcaccta gggaagcaag tgcggaagca aggtggaagc
    37201 caccttagaa gcagaggaat tccccatcat tccttaaatt cacgacgccc cgggcattac
    37261 cttacctcat ccatactatc catgacctgg acccaaaata ccgacccctt aaacaacatc
    37321 gcctggtccg ccctggtggc cgccctgccg gtaattctca tcttctggct gctgatcaaa
    37381 aagatgaaag gctaccaggc gagcctgctc accgttttgt tctcgatcgt gatcgcggtg
    37441 gttgtttatg gcatgccatt gaaactttcc ctcctttccg gtctgcacgg cgccctgtat
    37501 ggattgttcc cgatttgctg gatcatcctg agcgcggtat tcctgtttaa cctgaccatc
    37561 aaggccggcc aatttgaaat catcaaaaat tttatggctt cggtcactcc ggaccgcagg
    37621 ttgcaagccc tgctgatcgc tttttctttc ggagccttcc tggaaggggc ggcgggcttt
    37681 ggcgctccgg ttgccatatc cgcggccatg ctggtaggcc tgggctttaa tcctctttat
    37741 gccgccggaa tttgcctgat tgccaatacg gcgccggtgg cctttggttc cgtgggcacc
    37801 ccgatcatca tggcaggccg ggtctcagaa atcccggaaa tggccatttc ccagatggtg
    37861 ggccgtacgc tgcccatcct ttccgtcgtc cttccttttt acctcgtggt cctgatgtcc
    37921 gggttcaaaa agtccctgca ggttttcccg gccatcgtgg tctccggcct gtctttcgcc
    37981 tttttccaat ggtttgcggc aaattacctg ggccccatgc tgcctgacgt aatggccggg
    38041 atcgcttcaa tcttctgcct gatgatcctg ctgaaatact ggaagccctc aacggtctgg
    38101 cgcttcaacg aagaaccgga acaaaccctg gacagttcac ttaaatattc cgccggccag
    38161 gtgctgagag cctggtcccc gtttatcatc atgacgatcg tgatcgtggc ctggggactt
    38221 caacccgtaa aagacagtct gaatcacctg gggatgctcc gttttgatat ccctggcttg
    38281 aatggggcta ttctgaaaaa cgacggcagc cccctggtta tccgggcttt tgaattcaat
    38341 tatctttctg cgccgggaac ggccgtgctt tttgccaccc tgatttcaat ccccttgatc
    38401 ggactgacct atcgccaggg ccttgaaata ttccggatta ccttaaaaca actgagattc
    38461 ccgatcctga ccgttgctgc cgtggtcgga tttgccttca ttgccaacaa ttccgcgatg
    38521 tccatcacca tggcccttgc cctggccggc acgggcgccc tgttcccttt cttttcacct
    38581 atcctgggct ggctgggcgt cttcctcact ggttcggata cttcatccaa tgcccttttt
    38641 tgcaaacttc agtccacgac cgcggatacc ctgggtatag accccgtgat cacggtgagc
    38701 gccaatgcct cgggcggggt gaccgggaaa atgatctccc cgcaatccat agccattggt
    38761 gcggcggccg taggcctcat cggcaaggaa tcggacctct tccgttttac cctgaaacac
    38821 agttttatca tgttgttggt catttgcctg ctgaccaccc tccaggctta tacctgggag
    38881 gggctcattc cctcttacac aaaaccggat gggcagacga tggccgcggt agcggatgga
    38941 acccatggct ttacctacct catgatcatg gccctggccg tgctgcttct cgtgatgacg
    39001 gtttacctga caaaccggaa aagcaaggaa aggggaaagc cctgaacccc agctaagatt
    39061 tttttatttt atttgaatca agtacaaatt atgcatgccg aaaatctgat catccgggac
    39121 aatctcaagg acctgtatcc cgatgtttat accccggaag cgctttccag gctttcttcc
    39181 atggcctctt tcaaccggga catcaaagaa atcatggatc tccggatgca aagaaggtcg
    39241 gaacgccagg aaaaacaaaa gaggatcggt tttttggatc ccgaaagcct gatcccgggt
    39301 acttcgatcc gggtaggcga tgcccgcgaa gggcgatttg aaggatcggc catacccggg
    39361 gacctccagc ggcaatggat ccagggcacc ggaccggctg ccaaacccaa agcccccctg
    39421 gagagcagca tccgtaatgt agcctatgcc ttgctttccg gcgcggatgg ctggatgttt
    39481 gacggagaag atgccctcgg gcagatcaat accatgtcgc tggacaacca gcggaatctc
    39541 aagctggcga ttcaccggga ccccgtattc ctaaaagtgg cggaacaggt ggcaaaagaa
    39601 atgaaccgct ggtccatgga tttttttggc agggccatca tcagcgactg ggagcaacaa
    39661 ttaaaattta ccactataat attcagggcg cgcggccttc atctcgatga ccggcatatc
    39721 cgggaagccg gtggcctggg cctggcggct tccatcgtgg acctggtatt gtacgtggtc
    39781 aacaatcacg aggcccttgc cggggccggt tcttccatcg tcctttacct gccgaaaatt
    39841 caaaccgcag gggaagccgc tttatggaat gatatgctca ccgccctaga aaacctgctc
    39901 gggctgcagc acggtaccat taaggtgtac gtcctggtgg aacaactgga agctacctac
    39961 cagctgatgg agatccgggc cgccctgggc cagcatttta ttggcttcaa taccggacgt
    40021 tgggattata tcaacagcgt tgccgatgcc atggcctggg acccgtcctt catcaatccc
    40081 aatatcgaag ccattaccat gacctatggc tacatgcgca actacgaaga ccgcgtgcgt
    40141 cgggcggtaa acactcccga tctcagaggc ggctatgccc tttggcaggg gggcatggaa
    40201 cccaacatcc cggtgggttc tgccgaaggg gtttcggccg gaatgaaaaa agcagtggca
    40261 ggcgctcaac gggagcaaca tgacggagca agcggaaaat gggtgtccca ttggaagatg
    40321 gtgcatatcg tgaggccggt ttgggaaaaa gccggagccc tgaaccaggc cggcagggcc
    40381 ttcccgccac tcacttacac ccaggcagat gcggacgggc ttgtccttct cgaacccgcc
    40441 cccaggtcca tccggggcgc ccgcaacctg atttccgtag cccttcaata cggcaatgcc
    40501 tttctgcaag gcatgcaggc cgccgccctg aagccagccg attttttcgg agacgaggat
    40561 atcctttatc tcatggaaga catggccacc ggggaaatcc gcctgagcat cctctgggaa
    40621 tgggtgcata aaggcgcggt tctgacagag gatgacgaag cctccgggat cagggcaggc
    40681 agccggtttg attcagattt attcatccgc ctactggatg aggaatacca aaagttgctc
    40741 tccgcaagtg acaaagacgt gcacgacgat tcgaagacca ccaccttgcc gattgctcgg
    40801 gagatcgcgg cagcctatgt ttcggaagaa atcaaggctc cctggtttat agacctgctc
    40861 aacctcaatc tgaataatca ggatctggaa agggcaaaag aaagaatagg attgtacctg
    40921 gataccttca gaaaggaagg caggcggatc acggaaaatt taaattttta accggagccg
    40981 gattttactt taccgacgga accatttacc gtgggattaa cttggaatac tttctccttt
    41041 tctccttttt ccacgtttgg atttaaaatt gaatgcgacc tgattttatc gtattttcag
    41101 ttaaataaac cccaaatatg agatcttatt ttatcctcgg gctgtggatg tttgccggga
    41161 tctgcctggc ccaaaaacct gccgctccat tgacttccaa tttgaatttg tcaaaaagca
    41221 atatcaacag gttgatctat ccggagccat tgctcactgc tgtgaatgcc cggctgttgc
    41281 tggcggatct cgaaaaaatc aatcctccgg atgaggctgg gctgaaaacc tggctggtgg
    41341 ctaattttaa aagattcggt atccaaccgg atcaggtaaa acagattgtc atttattcaa
    41401 gcagatcgta ttcggattgc accatctgta aaaaaaattg caaggggcat tgtgtccagg
    41461 accccggttc agattgtgta tgcatccata aatcagaacc caacctcagg accatcgatc
    41521 aggggaaagc gggtaacgtc atttatttct ccctttcagc cgtaggtgaa aaagaagccc
    41581 tggataaaat tttaaatacc atccaggctc aaaaaacggc ggccgttaca aattaaaaag
    41641 ccggatttaa ggacaaatcc ggcttcatgc tttgattgat tatcttttaa ttcagcggaa
    41701 tccttttggc attggccgcc agccactggc caaaggattg caattccgga ttcagttctt
    41761 tcgttttggc gatatcgcgc ttctcggtaa acatctgttc aaattcctgg taaaactgaa
    41821 acatattgcc caggtcttcc gaaccaggga atccaaaact ccggtaaacc gcagggctca
    41881 ccgcattgta tctgacctct tccccaaaag ctttggtcat ggccgctgcc atatcggcac
    41941 aggtgatgtg ttcgccggcg atggctacgg tctgcccggc catggacggc ccttttttga
    42001 aaatgccgta agcgcatttg ccgatatcct ccgccgcgat gcccgacaat cttttgtctg
    42061 ccatagggaa agtgatcgcg aggaccccgt cagggccccg ttttggaccc atcccgaaat
    42121 agatcatatt ttcccagtag aaggtggtcg ccataaaggt cgtcgggacg cctaatctcg
    42181 tgaacaactc gttggattct cctttcgaat caaaatgagg cacggaatat ttgcccatca
    42241 gggtgggcat ccggtcatcg tgtaccggga tccagtcccg ggtatcttcc agggtggacc
    42301 agatcacgtg cttcaggccg gtggctttgg cagcctcagc catcactttt gcatcctggg
    42361 cttcacgctc cggggaaaag tgctcccaga aaaaggtcac acagaaagcg ccataagccc
    42421 cttccatggc tttgcgcaca ctttcaggat cggtcaggtc tgcttggaca acctccgctc
    42481 ctaatccagc cagggctttg gctttttccg aatgggcatc ccttgtgact gcccttacgg
    42541 caaattcact gttgggatcc gataaaatgg cacgggcgag cccgcccccc tgggcgccgg
    42601 tggcgccgaa aatggtgatg atctttttct gtggcatatt gaatttttag acctgttaaa
    42661 caaaaccagc ccgaagagtg ttcaccaaac ctatttcttt ttgtttaagg gccgggtagg
    42721 aaattcttgg atgtggtttt aataccccgg actcaggatt attttttcaa accaccgcga
    42781 tcctccttta atgatttcca ggaggtaagc gccttcgtgc aggccttcta aatccagatc
    42841 ctcccgattt tgaaaaattt ttatcctccg tccgttcagg tcataaatcc tggcctccat
    42901 cccccgggca tcggacatgg agatgcggac cttgtccctc gccggattgg gatgtatccg
    42961 gatggttttg cgggtgagtt ggtgcaccgg ggtcgaagga caatttaatt cccctatttt
    43021 tttccagatc tcattgtgta aatccagcgg caccaggtct tcttccccgc tgaccccctg
    43081 acggatcatc accatattct ttgaaaaaga aatactgacc agctgtccct gggacccccc
    43141 ggccaccacc acgtccgccg gtgcatccgg ggcgatcggc ccgggaatac tgagcgggga
    43201 agtgggtgcg ataaaggatg attttccatt gagccaccag aggtacccat aggatggatt
    43261 gagcgtttgg gaactgctta tcatctggtt gaaaaaggcg gtgtcctgga tcacggtcgt
    43321 attgccccat cttcctctat tgagtataag caggccaaag cgggccatat cccgcgcggt
    43381 actgtaatac aggctattgg tcccggtgta gatccagagg ccgcccatac cggttttcaa
    43441 ctttattttg gaggtcatgt acgggttgag ggcctggccg gaggctttct caagcaccgc
    43501 tttggtcagg gtataggggc cgttgtgata aacccagcgg gtgcctgcgt cggcggtgta
    43561 aaccaggcag tttggattga cgcaaaaaaa gaacagttca ttcagccccg aagtcatggt
    43621 caattggtgt cggatggtga ccttatcttc cttttccgcc ggcaggctgg tccagttttg
    43681 tccaaggtat ttggacaccc ggtcatcgag gtcgagaaat ccttcttcct gggcaatccc
    43741 aaccaatacc gctttcaggc ttttgcccgc ggaataccag gaccagaggc tgtcccgggt
    43801 ataggtcccg aaatattttt caaggacgat ccggccgtct tttaatacca ggaaagactt
    43861 ggtttgctcc cggtgcagga agccgtacag ggagtctatg cggtccgggc accagccgag
    43921 ggtggaggga tccagggtct gccaggcatt gccaagaagg ggagggaagt agagatcctg
    43981 agaacttaca ggattggggc tcagggcagc gaggattaaa ataaatacaa agcgcatacc
    44041 ggtcagattt ctgaaaggtc ttatggttta aaggattaaa aatataacct tgaacacaat
    44101 ccccctatcg ctgtttaagt caggtgcatg tcaaaataca ggtctgtttt aggtgtataa
    44161 caagacctgg aaggtttcaa cccacataaa tacgaagtaa aaccttccag gtctcgttta
    44221 tccgaaaatg taaaactgca cctgcatgcg cgggatagac atacacccta taagtccctc
    44281 aaaggtttct tcagaaaaaa tattttcaaa tttatttgcg caactcaaaa gttgcatata
    44341 tatttgcaac ctgtgagttg cataataaac ataaaaatat gaaacaggac ctgttccaag
    44401 ccatcgcgga ccccacccgc agggcgatct tgtctttgat tgccgtccag gcattgaccc
    44461 cgaatgccat ggctgaaaaa tttgatatga gccggcaggc cgtatccaaa cacattaaag
    44521 tattgcacga gtgcgaattg atcaaacccg agtatgccgg cagggaaatt tattaccatt
    44581 ttaatccgaa aaaaatgcat gaattggatc tctggctgga ccaattcagg aagatttggg
    44641 aaacccaatt cgatcaatta gaccaagtat tattaaccct taaaaaaaga aaaaaatgac
    44701 gaactctttg ttttttgatt ttaccgtgga caaggcggct aaaaaggtat tcatcactcg
    44761 cgagtttgat gccgaattgt ccctggtatg ggaagctttt accgtaccgg aaatactgga
    44821 ccaatgggtg gcgccgaaac cctttacggc caggacaaaa tacatggact tcagggtggg
    44881 ggggcggagg ttttacgcca tggtgagccc ggaaggacat gaacgttggt taattcaaac
    44941 ttacacatcc atcagcccaa aaacccattt caaattgttc aacgcgtttg cggataagga
    45001 tgaaaacccc gaattgccgg gttccgaatg ggattattcc ttcagtgaaa aggatggtaa
    45061 aaccacggtg cgcgtgacca tttataatga ttccctggcc cggatggaga agatgattga
    45121 actgggcttc aaagaaggat tcacggcgag cctggccaac cttgatctgg cattggaatc
    45181 ccggaagcgg tcggaatcat taaaaatgga tgaaatgaaa aaataatccc ccgttattgt
    45241 ttaacatgca atcattatgg acgtaaaaga acaaatcaaa aaccatattt cagaccaggc
    45301 tgaacccaaa cggagtgaac tgcaggaatt gcaccgtcga atccttcaaa tagcgccaaa
    45361 aaccaggtta tggtttttcg acggcaaaaa caccgagaat cagacggttt ccaaccctac
    45421 cattggttat gggtttcaca ccatgaaata cgcgaatggc aaaacagcgg attgctttaa
    45481 aatcgggcta agcgccaata aaaccggcat ctcggtctat attctcggca tccaggacaa
    45541 gacctatctg gccaaaacgt ttggggaaaa actgggcaag gcgaaggtga ccgggtattg
    45601 catccggttt aaagccttaa aggatataaa cttggaggtt ctcgaagagg ccctgcggta
    45661 tgggattgaa acggccgatt gactgcttgc taaaacagat acttcatcga atgccgcgcc
    45721 gctgagagga gaccggattt tatgggttta tttgaagagt taaaaaatca tgaagaatta
    45781 aatcggtata tttcggggcc tctaataatt agaaaacgta tgatcaataa taacaaaggt
    45841 attaaatgaa gaagcataaa atgatctttt ggactgcaat cacagttgca gtaacttttt
    45901 taaccacttc ctgttttaac aatatgaaac acaacacgat ggctgaaacc aatcaaaaaa
    45961 taacgcccta tttgtgggtg gagaaagatg ccaaagcggt ggcagattac tacttatcca
    46021 tttttaaaaa tggtaagttg aaagattatc gaaagttcaa cattgacgaa agcggaaatg
    46081 atgcagggat agaaactgcg gtcattgaaa tagaaggaat ggagtttagc atattggcgg
    46141 ctggtccgtt attcaaattt aatgaagccg tttcatttgt gatcaataca aaagaccagg
    46201 ccgaaacgga ttattattgg gatgcactta ctgctgatgg cggtgaagaa ggttcttgcg
    46261 gatggtgtaa agataaatat ggattatcct ggcaagtggt acccacggaa tattttgaat
    46321 taatacataa tgaggatcct aaaataaggg aaaaagcaat gaagaatacc ttgaaacaga
    46381 aaaaattgat acttgcagaa cttaaataaa cttaaaaata tcggatcgga tggaggggtc
    46441 ggacttctga ttcgattata aaccaatact gccaatattt tatttcgatt ggatatatgc
    46501 gattttataa aaatggcctg ttcaataagg gaactatgac gggccctgta ttaatgatcc
    46561 tatgcctcac catagctgtt attcccattg atggattcag tcaaaccaag cggctgtgga
    46621 ccgaatccga tcgtgatttt ttagtcagcc atctggaacg aacgaagaag gaaatcataa
    46681 acgaaacaca aaatttaacc ctgcggcaat ggtccttccg ggaagattcc gccaaatggt
    46741 ccataggcca ggtcctggaa catttgggat tgtatgaaag gatatttatg caggaggcgg
    46801 acatcatgct cagtacaaac cctgaaccgg aaatggataa aaacgccctg ccggacagct
    46861 cctatatcca ttggatgaat gatcccagcc cgcacaaagc ggaatggaac gcggaacctt
    46921 tgggattcat ggacggtaag gagaatctta acttcttttt attcggcagg gatcgtatta
    46981 ttgactttat caggaatacc acctatgatc taaaggccca ttatactttc cgctggggca
    47041 gcgaaaaaag acgcagcatc catgccttga tggtggttca tttcgcccat accgaccggc
    47101 atttgcggca gatcagccgg attaaacttt cgaagaattt ccccgaataa aaatcgtcca
    47161 cagcatctga atggatggaa aattactggc cggaccctga aaaaaggccg ggagagacca
    47221 cgcataaaat atgccattct atacggtcat ttatttgagg ggtgacggta tggaattgca
    47281 tcaagcagag accgggacaa tgtaccactg acaaaggtct tggccgagag tttcgtcagg
    47341 taaatcggta tccacgaact gtattcccac ttatccaggc cttttttatt ctgctttaat
    47401 cccgggtaca aacgcgtaca gacagataca aacggctaca aacggccaaa tccttttgta
    47461 ttcgcttaaa gccgatgaat aaaggaagga tcacaaaagt ataccgactg tccctaatgg
    47521 gcatcctgaa ggtttgagat tttttttggg cctgtcggat acttttataa atgttatgcc
    47581 tcatattgaa aaaaatatta aatttatttg aattaaagta gctaatatgc tacctttgac
    47641 ttatgaaagc ccttgacctt gaaaagctga tagaatcaaa agggctggta ttttattaga
    47701 caaagtggaa gtcataaaat ttacaaacat gaaacaattg ctggaattat agtaattccg
    47761 tttcatggat caaaagattt accaaaaggt acagaaataa gtatattgaa aaaggcaaaa
    47821 ataaagaagt agtatgaaac aattaacagc gattatcgaa aggaataaag atgcattttt
    47881 tgcatatatt aaagaaatag acggctgtgt tgctggcgga aaaaattatc aggaggttaa
    47941 attaaattta gacaaaatgc tcactatagc ccaaaaggaa gataaggatc ttaaaaagat
    48001 attaagtagg ggttacaaac taaaatttga agttgattta gaatctgtat ttgatttgat
    48061 tccagaagtt aatataagcc aattggctaa aacagggaac ctaaacccag gcttacttcg
    48121 tcaatatgtg tcaggcacaa aaaaagcatc tgaagctcag acagagaaag ttatgaaagc
    48181 tataaaagat gtaagtatga aattaaattc aattacgctt acagtcagtt aaaaaatacg
    48241 aggcataaca tgagattgaa tcaagtagag caagcatctt tcattcaaga aaaaaccgta
    48301 aatttggcat aagttgttta ctcttggcag acatcgaaaa ttctctgcct gcctcaatct
    48361 caaatccgtt atgccttatt tagaaaaaac gacaataccg taagccaatg gaaattacac
    48421 ttcctatatc gagtttaatt gtcattgcca taggcagtgt gggaatagcc gtatcaattt
    48481 tcatcggtat tatgttgcaa tctcaaaaag ataagaaaca catatccata agcttattgg
    48541 cttgcttgct cattctctcg ggattgaccc tattgaatga cgtcttagtt acatcaggaa
    48601 tcagcaaccg aatcaagcaa ctttactttg ttcctatcta ttactcattg agtgttgccc
    48661 cgctttttta ccttttcatc aagtcaaaat tcagttatcg cttaaataaa tctgatttac
    48721 ttcatttggt cattccgact atacaggctt tcgtttattt ttcgattggt ttccgttccg
    48781 ttgcattcaa atcaatgctc tatgacgaaa atgcatttcg gatgtacctt caaatagaat
    48841 catttctttt cccaattttt ttggtgggat atactgttct tgctttgctt tttttacaag
    48901 gccaagacca ggaaaaatat ttttggacaa aagatataaa aaaatggctc tctaattttt
    48961 cggctggaat gttattgata gctatcatgg aattttgttt ttcattgatg gaatacaata
    49021 cctctgctaa ccctttgcct ggctttccat tttatttagt tcatacattt acgctttctg
    49081 cttttgtttt ttggatttcg ataaacggat tcaaacagta ttatccctta cagattttta
    49141 tttcaaaacc cagccgacaa gaagcgaata ttgacgaata tgaattgtcg aaacttttag
    49201 aaaagctgaa attgttaatg actaacgaca agatttttct taatcctgac ttaactcttg
    49261 aactgctatc gaactatctt gggatttccg aaaaacaatg ttcttccata ttgaacaaag
    49321 gaacgaactt gaacttcaac caatatgtaa atgaattccg aattaaagct tttaaagaaa
    49381 aaatcaaaga agggcaaaac aagacctata ccctcacaag tattgcgtat gagtgtggct
    49441 ttaattccaa atccacattc aacagggtat tcaagtccac gtgtggagta acaccctccg
    49501 aattcgtcaa gatgacgacg caggacacaa ccgtatccga ctaataattg cactaaaact
    49561 tgtcccaaat cttgatttga catgtcccaa atcttcattt gaagcgatca agcggatgtt
    49621 agctctcatt tttaccactc accaattaat gtagtaatta tgaatagcaa aatcaaagcc
    49681 ttcctttttg gtggcaatca tcaaaactct attagtcaaa acatcggctt cttactcctt
    49741 cgtttctttg taggacttgc actttgcaca gtcttcgaaa agttctttcc aaaaaacggt
    49801 atttggggac ctcaagattg gttcattcaa gataccgcta atatgggttt cccgtttcca
    49861 accttctttg cctgggctgc ggtaatatct gaattctttg gcggtatatt actgatgctt
    49921 ggcttgttta cccaacctgc cgcactgctg aatattttgg taacttttac agcaacattt
    49981 atctatcata aaggagatat tggaaacgca ggattactat cattcttttt catgattatg
    50041 tgcctttgta tcctactttt tggaccaggg aaatttagcc ttgactacta tattaataaa
    50101 aggatggcca aaagaatgac aaccatcaca acgattgttt tattaatttt ctcatttaac
    50161 ttcggagaag cgaaggccaa tagacaatca atcccttatc agatagaagt cctaatcgca
    50221 gactcttcgc aaatacgatt ttgtttgaaa aacaatagct tatttccaaa aaaacgaatt
    50281 ttcattatct accaactcca acaaggagga aataacacga ttgttagatg gtttttacct
    50341 tttcaaaaga tacaatttga tgtgccaatc ggttccaaaa tctatattgc tgcggattca
    50401 caggtagaaa aggtgatgca agggcaacga atagacagtg ataaaccctt tttaaccgag
    50461 gatgatagga ttaataacat taccttgcaa tcgcaggata gtgttaaatt taaagttctg
    50521 ttcttaagtt cttaagttct tcgtaggaag ttcagtggtt aaaatccctg cctgcgtata
    50581 gctgtgaact tttatgcaca agactaaaag accgaccaac agtaagcaat gacacactcc
    50641 actgacatat attttcctac aactgaaact gaaaagtatt ttatcaagag catttggcga
    50701 cttcaagaat ttaataatca caacctgacg gagaccattt tgccaaaggg aactgttgaa
    50761 ctaattttca atctatcaga caaaatcatt tatctaaact cggtagcaga tgtaaggaca
    50821 accttgcctc catgttttat caacggtatc aattttaaac cattccaatt aatcaagaac
    50881 ggacaacaac tatttgtggg cattcaacta aatgtcatcg cactaaaggc cttgtttgaa
    50941 attccggcta tggagttcaa tgatgctatt gttgagagtt cgcaagtatg taaaccattg
    51001 agtgatttat acaatcagtt gttttcagaa agggcgtttg aaaggcaagt gcaaatcatt
    51061 aggaaatggc tataccataa aatttccact tcaaggtatt tagcagcgct tcaaaaattt
    51121 tacaactggt tctatttccc aaatgttaac accatgacgg taaaagaact cggtaataga
    51181 gcttgcattt ctgaaaggca attgagacga ataaccactg aatggctggg aatgaacacg
    51241 gaggcatttc tactctataa caaatatctt tcgtctctac atctattgca cagttctgat
    51301 ttatcactta ctcaaattgg cttggaagct gggtattatg accaatcaca ttttatccgg
    51361 gaattcaaat cctacacaga cctgacacca aaggaatatc aagcatctgt taccggctta
    51421 ccggggcata ttttcggata gattaaaaaa tgtccgaatt atacaatttt cacagttact
    51481 ctgtttctaa atttgcttta aatttttaat ttattatata cagatatgat gaatgctgtc
    51541 aaatttaaaa gaaccgtcgg cgggataact gtattatctg gtatcatcgg atttaccagt
    51601 tactttttag tggccggctc agtaagtttc aactttgaat tcttttcaaa cccatcgctg
    51661 attttttcta tgcccgatgt caacattgtc atgctcaggt ggagtatgat tacagacctt
    51721 tttggatatt acctattgct gcttcctttc attttcttta tgcatgaatg gttattaaat
    51781 aagtcggcat ggagaaaatt atttactttc tgcggcactg catatgtaat ggcaggtgca
    51841 ataggagccg caatactcgc tgtaacttgg cctgcctttt taaccaaata tcccggttcc
    51901 ccacacgagc aacaagaaac catcaaactg ttgtttgaaa gcttttcgct gatggtaggg
    51961 aacggaatct ggaatttgtt tgataccttg gtttgtggtg tatggatgat tggcatcggt
    52021 gtttatatta agcgggtgaa atcattcttg ggatggttta ccgttgctgt aggcatcatc
    52081 tcgttgttgg atggttttgg caacatgctt gaattatcgg aggtatcgga ccttgcactc
    52141 aacctgtttc tttttctagc gccgctatgg gctatattta ttggttttgc tgttatgaga
    52201 aattcgttct cactggaaaa atcataaaac gaccaaaaga tgatttacaa attctcctta
    52261 atagaaaaaa tgcttcttgc aaacgatgtt atcccacatc cgtttgctga cgcttcattc
    52321 tccgtaggtc tgggttttgc cttgggtagt tcaataaaat tgaaaattgc tgaccagctt
    52381 tcaatggagt ataaagaggt cgggactatt gcccatgcag ctaatgtaag tgaagtagga
    52441 gccgaactaa ttcttgactg cttagacgct ttaggatacg ttcaaaaaaa aggcagtcat
    52501 tatgccttta caaaaagagg ttacaaaaat ctatcgccaa aatcgcctca taatttccga
    52561 tattttattc tattctgtga ttatttgtat aaaggatata tcaatttaga cgaaaccatt
    52621 cggcttggga gaacagcgaa ttccaatatg cttgaacaaa tgacggatta tgagtgggaa
    52681 ttgttttcta gagccatgat agatatttca agcacaaatt ataaagaagt tgcaggaaaa
    52741 ataccagtag caaaaagtgc tcaaaaaatg ttagaccttg gtggttcaca tgggttatac
    52801 agtattgaac tttgcaaccg aaatccaaat ttaaaatcta caattgttga cttacctcct
    52861 gtgaaaaaat acgctgacga atgcatcgcc caatataatt caggtaaaac ggtttcattt
    52921 ctcccagcag attttatgaa agatgaactc cccaaaaatg ttgatgtcat tcttgctttc
    52981 aacattattc atggactcaa tccgtcagag aacgagacgc ttgctcaaaa ggtttatcaa
    53041 tcgctaaaca gtggaggaat atatgtaata cttgaccaga taaaagggat tggaggaagt
    53101 tcccaaatgt caaaagctac cacttcatat atggctctga atttattaca tcaggctggc
    53161 ggaaagacct attcaaaaaa tgaggttgac acattcacta aaaaagctgg tttcaaaaat
    53221 acagttctta aaaaattgaa tgctccaggt tttggtgtaa ttatttgcac taagtaaatc
    53281 gtaaatcaac aatcagagcg taattttatc tttgactaag tatgtatatt ccgggccatc
    53341 taaccccctg ttccgaggga tattgacccc cattccgcga tactgacccc tcggtaaaaa
    53401 ggtaaaaaag ggcattccgc gatatttacc cccacttaag gacttaaggt gttcattccg
    53461 ggatactgac cccgcattcc gggatactgc caccccccct taccttgggg taattacggg
    53521 ttccgagacc tcggaactaa cgaactgatt tcataattga atctggattg taaccgtgtc
    53581 aagcgtgctt tgcataaaat caaagcacag cggtatggca ggaaattcaa tccgaagcct
    53641 cggatcgtat gagtcaactc aaacaaatat gatatatttt gaagatggat tgaattcttt
    53701 gattaatttg gtatattata cttttttatg tacgaaatga agaagactgg aaaaactttc
    53761 attcccatga attgtcaaaa atgattctga gtaatttgcg ggggcttgca atatttggat
    53821 tggggtggtc cacgcaattc atcagcaatg aaacctcgac tttagtgtct gcatatttca
    53881 ttatgcatgt tctaagtccg ggcgcgggat catcgcctgc gtgccatccc ccggaatggt
    53941 acaaataagt tccatgttct ccgtttgccg agccatgttc attttcccag cctaataggc
    54001 cgtcatccat ctgtttgcgg gttaccggtg ccaggatgtc gttattgtag cggagatggg
    54061 ccatgaatgc agctaaatca aatgccgaaa gatgccagcc ataagctccg cttctcatac
    54121 gataatcccc tcctccgccg gtaacgtccg agacgcaatt atagtacttc atcggtgaat
    54181 ccggaagttg gaagacaaag gcatcaggta tgcccattgg tttaaatatt tcctcatgaa
    54241 tcacccagga ataaaagacg gcagcaccga aagcgtcgat ttgcgattta tctttctttc
    54301 cggccagtat gggaagaatg actcgaaaca atgaataatt tgcgtttttg tattcataat
    54361 ggttacccag agccgctttg tttatgcctc cttcaatcat tgatcttaga gccccatatt
    54421 cgtcgttgcc tcccgattga ataaaaccgg atttatgagt taataaatgt ctgaacgtga
    54481 gtaaatcaac atttggcccc cttacccaac ctgaaggtaa atagcgggaa attttactat
    54541 ctatgtttgc gcccggtttt ctcgctaaaa ttttcaggac tgcagcggcg gtgattgttt
    54601 tactcacact ggcgatatgc attcgcatga cgggagtgaa gggtatccga ttggcttcat
    54661 ccgcaaaacc ccggacaccc atgctataca tgatctgatt ttgatttatg gtataagcaa
    54721 agccgggcac ctgacctgtc atgctgtcta atagactttg ttcaaacaat tcaatattga
    54781 atttaggccc tgaagggaat aagaatttgt cccaattaca agatacgatg gtaaggctta
    54841 tgatcaaccc agaaataatt gttcgaatgt tcatggttcc atctatctat gattttctta
    54901 tgatgcctcc ctaatttaac tgatttcatc catataattt gctttctaag caaaatatta
    54961 ggtttaatct ccgtcgaatg gttcagcaat tctgccccgt taatcagaat ttagggcagc
    55021 tctaaatacc cgttcatggt ttgatcggct ttttaaatca atagcctaaa ttgctctttt
    55081 gtcccgattg gttcgggaga gagccctgct atgctccttc gattcgcttt gtctaatccc
    55141 gaacccatcg ggacaaaaga gctaattttc aaactcattc agggtattta gaggtgccct
    55201 ttaattttat taattactta tgtacttaat gtacttatgt tttcttacgt ccacttgtga
    55261 atggctcatg aataacagtt ggtacccctt cagtgtctgc cggaggcagc atcttctatt
    55321 aaaggcccga tcaagcagga taggtcattc cgtcaaggca aaagctaaat atacatccga
    55381 tgaaggcgtc ccattccggt tcccgccacc ggcggcaatg acgacatatt gtttcccctt
    55441 aacctgatac acggagggtg tggcataacc gccggcaggt aattgaaagg accacagttc
    55501 tttgccatcc gaagcccgga aggccctgaa cctttcatcc gccgtagctc caataaatac
    55561 caggcccccg gctgtaactg cacatccgcc gaaattctgg gtacctgtgg cgcccaggcc
    55621 tttttccttt aaggcgggat aataacccag gggaacctgc cattttagcc tgcctgtatt
    55681 catatccacc gcattcagcg tgccccaggg aggacgtacc cctggataac cgtccttgtc
    55741 ggtgagtatt ttataacttc tgagtacaaa tctttcggat cgttcaccgg gtccgtccgg
    55801 atttacagtg ctatgatctc cggatccggt attttcatcc aacagaaagg cagccagggc
    55861 atccaactcc ggttcggaga attggctgta agcgggcatc aggccctttc cccgggtgat
    55921 gacaccggtc aatgcggatt ttgaatattt tttgccaagg ccggtcaaca tgggaacggc
    55981 ctggttttgt cctttacggt cggcgccatg gcaggaggtg cagttcaaca tataggtatt
    56041 atagcctctg gcgagcaggg aggcctctcc accgccgggt ggcactactt tctgaagttg
    56101 gaaaatcatg gcgaaatcat tgcaattggc ataggcaatg cgttggaccg gatcgaatga
    56161 aatgcctccc cacaacattc ctccccgggt acccggcatt ccaagggttc cctggaggct
    56221 cggaggggta tacaatcctc ctccctggta ttgcgcagat tctttcaaaa caaaggcccg
    56281 ggccgaatcg gagatattgg tcaaatagct ggtatcccaa ccctgtcggg tgagccggat
    56341 gccctgcccg aaaggctgac tgggaaaggc cgcttcaccg ggcaccccgg aagccggcac
    56401 ggatctttct tccggatcca gcagggcctc cccggtatgc ctgtccagga caagcaattc
    56461 tcccatcttg gttggctgaa gcagaacctc cctggacctg ccttcccatg aaatatcggc
    56521 cagggtgggg gcacaaggaa gatcatagtc ccagatgtcg tgatgcacgg cctggtaatg
    56581 ccatttcaat actcctgaag cggcgtccag ggcaaggata caattgccaa acaggttctg
    56641 accaatccgg tttgccccat aaaaatcata agtcggcgat cccgtggaag catatacgac
    56701 tccttttttc tcatccaggc tcatgccgcc ccagttattg gctcctccat aattctcccc
    56761 ttccatccag gcccagcttt catgacccgt ttcgccttgc ccgggaatcg tatgaaagat
    56821 ccaggccatc tccccggtcc cggcatcgta agcccggata tggccgggcg aggcgtcgta
    56881 gccttccccg gtggcagaac cgataatgat cagattttta aaaataatcc cgggggtggt
    56941 cagggaaaca gacaatttgg cggtatcctt tccgagaccg attctcaaat caatttttcc
    57001 ataatcccca aaagattcca caagctttcc ggtgccggcg tcgatcgcat agaggtaatg
    57061 tccgacggga aggaagaggc aggccttgtc cttggttttg taataggtca ggccccggtt
    57121 gatcccgcct cctgtagcgg attcagccgg ttcatatctc cagacttcct gtccgctcgc
    57181 ggcatccagg gcgatcaggt ccaggccggg tgaggtaata tacatcctat ccccgatgat
    57241 gatgggattg cattcgatgg tcgttctgtt gccggagtcc ccggtatgaa aggtccaggc
    57301 cggttggagc agatgcacat tggaagtatc gatttggtga agtgcagaat attgcagggc
    57361 atcgggactg ccccggtaag tatcccaaac catatacttt tctccccggt ccgccttaca
    57421 tgaaaataag attattgcgc ttgcaaccag aaccaggaac agaggtgtaa ccgaagtatt
    57481 taccggcaaa aacttcaccc aggtcatttt catcttcatt tggtctgacc cgttttaaaa
    57541 tgccggctgg caaagcggag gtattgtttc caatcataaa ggaggatatc gtgtttgccc
    57601 gccctgatat ggtaggcgat ggtttcaccc accggctggc caatgggggg ctgctgttcg
    57661 gtcatcaggc ctttttggcc ataaagggca tataccggcc cggcatgcag ggcacccagg
    57721 aattcccctt tgggatcagc ccaaaggtcc tcggaagcgc tggccacata gaggggacgg
    57781 ggggccatga gggcaagcaa catatgctga tccaccggga gttggtccac cgcatgattg
    57841 taggtcttgt atttttcaat aaaccagtgc ggaaaagcgg tattgatccg ctggacggtt
    57901 tctccaaaat tgcggcgggc cagggccgct ccgccttcgc ccgaatcgtt ggaaaccact
    57961 atggcaaacc tgcggtcgtt ggctccggcc cataaagcgg ctttgcccag tcgggaatga
    58021 ccggtgagca cgacctgagc cgggttcacc gccggttccg attccaggta atccatgatc
    58081 ctgctcagtc cccaggacca ggcgccgatg gctccccatt catggagttt aatttccaat
    58141 tccttgctta gagtggtccg gattcctgtc cgccagcctt cgggaaggtc gggttccagt
    58201 tcttcataat aagcggtgac cagggcaaaa ccgtttgaca ggatttcttc cacgggccag
    58261 cggcgggctt gcccaccgcg ctcggggttc gtagtccctg gatgcaggct tttccattgg
    58321 tcggtgatgg ttatgcccgg atcggtttgg gtggaatgat tgccctggaa attgaggcca
    58381 agacatacgg ggaccgggcc tttatgattt tttggaaggt acaccaggag aggaagcgct
    58441 gggccctgat cgcctttggt gaaatacaag gtaatctcct tccggatggc tttaccgtcg
    58501 agggcattgg gttccagggc attttcttta aaatgaaaat cttttggacg gccgggcatc
    58561 cttccgtaaa catgggtggc aaatttttgg agaatggcgg gtctttgttt tttttcccat
    58621 tcttccgcag agcggatggg atggccttcc tcggtgacca gcagggacgg caggacatat
    58681 tggcctgccc ggtcttcatc ataattggca gggtcctggg ctgggatttg gtgaaaaagg
    58741 attgcggatg cggcgaaggt caggatggag cggcggatca tggcggaaag gtttgaatga
    58801 gctaatttag agattctccc ccaggccttt taaaataaca atcccggccc aactttccta
    58861 ccggcacaga tggaccattc acctaccttt gcctgccaaa ccgataaccc tttaggtgtg
    58921 caattatgaa aaaacacaat tttaacccgg gcccggccgt attgccggcg ccggtgctcc
    58981 aggaggcttc ccaggccatc ctcgattaca atcattcagg cctttcgatc ctcgaaattt
    59041 cccaccgcgg atcggatttt gtggccattc tggacgaagc cataagcctg accaaagaat
    59101 tgctgggact gtctgatgat taccacgtat tgttcaccac cggaggggcc agctcccagt
    59161 tttttatgct ggcgatgaat ctcctcaatg aaggggatac cgcagcctac accgataccg
    59221 ggacctgggc taataaagcc atcaaggaag ccaaagctta tgggcaaatt aaagtcatcg
    59281 cttcatccaa ggataagaat tattctttca ttcccaaaga cctgggagcc ctgccggccg
    59341 atgcccggtt tcttcacctg accagtaaca ataccatttt cggcacgcaa atccattcct
    59401 ggccggaggt gccggtcccg atcgtctgcg acatgtcttc ggacatattc agcaggacta
    59461 ttccggtgga caaattcgga ctgatctatg ccggggctca gaaaaacgcc ggagccgccg
    59521 gggtgaccat ggtcattatc cggaaggaca tggtccgcac cgaccttacg caccacctgc
    59581 ctcccatgct tgattacaaa acctttattt ccgagaactc tttgtacaat acaccgcccg
    59641 tattccccat ctatgtaagc atgctgactc tgcgctggat caaaaaactg ggcggcctgg
    59701 aggccatgga aaagatcaac cggcacaagg cgaaactgct ttatgatgaa atcgacgcaa
    59761 atccgctctt caaggggacg gtcgctgtcg aggaccggtc cctgatgaac gtatgttttg
    59821 tggcggagaa tgaaaccctg gaaaacgaat ttgccgccga agccaaaaat gccggttgtg
    59881 tcaatttgaa aggccatcgt tcggttggag gcttccgggc atccatttac aatgccatgc
    59941 ccacggagag cgtccaggtc ctggtggata cgatgaaaga atttgcaagg aaaaaaggtt
    60001 aatttagccg ggcatacatg gtcccgcttc aggaagaaac gtatatcgaa tataccggcc
    60061 atcgtattcc attggtcatc atacgcgaga gaaggaggac cgtaagggtc gccttcagcc
    60121 gtaaaaccgt caccctccgc ctcccctgga ctttggcggc ttcggagaat aacaaacaca
    60181 ttgaaaacag taaaaaatgg gtgatcgacc agctcaaacg taaaccgggc ctgatccacc
    60241 atttttatac ccgcatctac cggcacggcg agaccatccg ggtcgccggc aggcaatatg
    60301 tgctgtatat gctggaacac gagggcaaga ccagcaaggc tcaacttaaa gggcaattga
    60361 ttgaactgaa gctcgccccc gggaaggaag aggaaaaaaa tgagatcatc aagtatctgg
    60421 tcagccgcct catcgccaac gattgccatg aaatggtcga ggcccgggtc cgggaactga
    60481 accggatgac ctataaccgt cccatccaaa cggtgcgtct caaactgaat cagtcgaact
    60541 ggggctcctg cagcagcaag ggaaatatca acctatcggc ccgcctgctt ttcgcgccca
    60601 gggaggtgat ggactatgtc atcatccacg agctggccca cctggtggaa cacaatcacg
    60661 gcccccggtt ctgggccctg gtcgccaaag ccatgcccgg ataccagtcc tgcgagcaat
    60721 ggctgaagga taacagtcat ttgtgtgaat tttgaagtaa aacggttaaa ggcgaagggg
    60781 taagggcgaa aggagcaaaa agcggataac cgcttgtggg ctcctcaatc ctcaatcctc
    60841 actcctcaac cctcactcct caaccctcac tcctcaaccc ccagttcaca accctcaata
    60901 ttatcttacc ttagcccatc attttttaaa gcgcatagat gactgccatg caagccatat
    60961 cgccgggttg gatcctggtc ctgatcctgg gttattttgt catgctgatc gggatcgcat
    61021 ggtggaccag ccggggcagc gcttcgaatg aaaatttttt tctcgccgga cgtaatgctc
    61081 cctggtacct ggtcgccata ggcatgatag gggcttcgat cagcggggtc acctttatat
    61141 cggtgccggg gtttgtacag gccaaccagt tttcttattt ccaggtggtc atcggttata
    61201 tattcggata tatggtgatc ggcctgatcc tgatgcccat gtactaccgg ctcaacctca
    61261 cttcgattta cggctacctg gaaaaaaggt ttggatggaa atcgtataag accggctcgg
    61321 cttttttcct cctctccagg tcggtgggat ccgcctgccg cctctacctg gtggccctgg
    61381 tcctctacca gtttgtgttc ggtcccttgg ggcttccctt ctggctgacc accctcatca
    61441 ccatttttct gatctgggtg tacaccttcc ggggcggcat caagaccatt gtgtataccg
    61501 atgttttcca gacctttttt ttattggctt ccctgatcat ttccgtgatc ctgatcagca
    61561 agagcatggg ctggggcccg ggtgacctct tcaccaatgt cagggaatcg gaatactcta
    61621 aaaccctgtt ctttgacagc ggatggagtg acaacaaaca ctttatcaaa caattcctgg
    61681 gaggcatgtt tatcaccatc gtcatgacgg gcctggacca ggacctgatg caaaaaaacc
    61741 tcagttgccg caacctggga gatgcccaga agaatatgtt ttccttcacc atcatcatgg
    61801 tcattgccaa tgccttgttt ctggttctgg gagccctgtt gtttctctat gccggcgcca
    61861 aagggatcga agtccccaca cgggaaatcg cgggggtgat gaaagcccag ccggacttat
    61921 tgttccccac gcttgccttg cagcattttg gcgggattat cgggatcttt tttatcctgg
    61981 ggctgaccgc cgccaattac gccagcgccg attccgccct tgcggcactg accaccgctt
    62041 tttgtgtgga ttttctgggt tttgaaaggg accgggtggc cggggaccgc atcgtaaaag
    62101 tccgtacccg ggtgcatttt gccttcagca tgatcctgtt tttgatggtc ctggtcttgt
    62161 ggtttatgaa cgaccggagc gtcatcgaca ccgttctgac cctggccgga tatacctatg
    62221 gtcccctgct cgggctttat gcttttgggt tttttgtaaa aagacaggtg cgcgacccat
    62281 gggtgccctg gatctgcgtg gcatcccccc tcatcacctt tattctgaat aaatattccg
    62341 cggacttgtt ctggggatac aaattcagtt ttgaattgct gatcctgaac gggctgctca
    62401 cctttgccgg cctctggctg gtttcctatc cgtcacccat ttcaccgaat ttactgggtt
    62461 ataaaaacag agtatgaccg accgaatcac cgaacaaagt tctcattacc gccacctcga
    62521 acaaatgagc gtggaggaaa tcctgaccaa tattaaccgg gaagaccaga ctgtgcccct
    62581 ggccgtggag aaaagcattc cccagatcgc agccctggtg gaggcgatcg tagccaaaat
    62641 gaaggagggg ggccgtttgt tttatatcgg cgccggcacc agcggccggc tgggcatcct
    62701 ggatgcttcc gagtgcccgc ccacttacgg cgttcccttt gacctggtca tcgggctcat
    62761 tgccggggga gacaccgcta tccgccgttc cgtcgaacac gccgaagatg atcccaaaca
    62821 ggcctggctg gacctggagg cctgggacat cagcgccaag gacatagttg ttggaatcgc
    62881 agcctcgggg accacgccct acgtcgtttg ggggctgagc gattgccgcg cccacgggat
    62941 cctcaccggc tgcatcacct gtaatccagg ctcgccattg gaaacaaacg ccgactttcc
    63001 ggtcgtcgcg gtggtgggcc ctgaatttgt gaccgggagt acgcgtatga aatccgggac
    63061 ggcccaaaaa ttgatcttaa atatgatcag cacttcagtc atgatccggc ttggaagggt
    63121 gctggacaat aaaatggtgg acatgcagat gacgaatgtg aagctgaaag accgggctgc
    63181 gcacatgatc gtgcagaaac tgaatgtacc ctatgaagag gctgtggaaa agttgcgggt
    63241 gcatggaagc gtccgggcgg tgctggatga gtatgagaaa ttaaggaatt aagaaataaa
    63301 ttaaggaatt aaaaagggtg tattcaataa agtgtgtcag gtacttcagg gcaggctgct
    63361 gagtctgagg
//
LOCUS       DAOQXT010000013        62467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_504_length_62467_cov_5.989585, whole genome shotgun sequence.
ACCESSION   DAOQXT010000013 DAOQXT010000000
VERSION     DAOQXT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 62467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62467
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_504_length_62467_cov_5.989585"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKM27_03545"
     CDS             <1..446
                     /locus_tag="PKM27_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR06362.1"
                     /translation="ILPYAREAGVKLAIEPLHPMYAADRSAINTLAQANEMAAAIDSP
                     WVGVAVDVYHLWWDPDLETEIKKCGRMNKLFAFHICDWKTNPEDLLNDRGLMGEGCID
                     LPLIRTWIEAAGFNGFIEVEIFSNRYWAMDQHRFLSEIKEAYLNC"
     gene            466..1626
                     /locus_tag="PKM27_03550"
     CDS             466..1626
                     /locus_tag="PKM27_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06363.1"
                     /translation="MKQHKIGIIMNGVTGRMGTNQHLMRSIAEIIKQGGVKTGADEVI
                     IPDPILVGRDENKLGQLCNRSGVDRFTTDLDLALSDEHNIIYFDAQTTGRRAAAVRKA
                     VRAGKHIYCEKPCAVSTQEAMELYRLCTDAGLKNGVVQDKLWLPGLTKLKRLIQNDFF
                     GRILSVKGEFGYWVFEGHTIPAQRPSWNYRKEDDGGIIVDMLCHWRYVLDNLFGKVQS
                     VSCLGATHIPERIDENGKAYACTADDAAYATFLLEGGIVAHFNSSWATRVRRDDLLTI
                     QVDGSKGSAVAGLRECYTQHYGNTPKPVWNPDIEQPIRFFEGWSRVPDQENYDNAFKI
                     QWELFLKHVVKDEPFPWTLLEGAKGVQLAEKGLESWEKRRWVEVEDLEVGGG"
     gene            1732..2502
                     /locus_tag="PKM27_03555"
     CDS             1732..2502
                     /locus_tag="PKM27_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HNR06364.1"
                     /translation="MSKTALITGGSRGIGLGIAVALSRQGFNLAINGVRPEPEVQEVL
                     EQLRANGKEVIYCQGDIASEEDRKSILARIKEHFGRLHILVNNAGVAPRERNDLLKTS
                     RESYDHVMDTNLKGPYFLTQAVANWMVEQKSLDPTYEAQIIIVGSISATVASVNRGEY
                     CLSKAGLAMLAQLFAVRMSEYNIPVYEVRPGIIKSDMTAGVTEKYDKLIAGGLTLQPR
                     WGTPEDVGKAVAAITSGHFPYSTGQIFMVDGGLTLLRL"
     gene            2534..3829
                     /locus_tag="PKM27_03560"
     CDS             2534..3829
                     /locus_tag="PKM27_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06365.1"
                     /translation="MKKIKRREVLKSVLPGALALTMPTIIPARAFGANERVRVAVIGF
                     NSRGKDHISNFMKLANVEVAGLCDVDKKVLQSTAADFEKKYGKKPKIAQDMRTLFDDK
                     DIDAVSIATPNHWHALATVWACQAGKDVYVEKPASHDFNEGRIMIRAAEKYQRIVQNG
                     IQLRSSAAIQEAIQHLRNGLIGNVYMARGVVFKWRPDIGDKGNSPVPEGLDWNLWQGP
                     AAERPFSENYVHYNWHWFWDYGNGDIGNQGAHEIDLCTWGLDVGFPDEVSCTGGKFLW
                     KDCKETPEVLSATFKYPKAGKLIQFEVRPWMTNREDELEVGNIFYGDKGYMTVNWYDD
                     YKTFLGQKREPGPSRKAGGDHYLNFIEAVRSRDKSKLNGPIETGHVSAGISHLANISY
                     RLDRSLKFDPKNEKFIDDAEANQMLSRKYRAPFELSEKI"
     gene            3835..5058
                     /locus_tag="PKM27_03565"
     CDS             3835..5058
                     /locus_tag="PKM27_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR06366.1"
                     /translation="MQHNHSTWRNLLRMPVLVAALGYLVDMYDLFLFSVVRVPSLTDL
                     GVSQDQLLPIGVSILNWQLIGLLIGGILWGIMGDKKGRLSVLFGSILMYSIANLVNGL
                     VQTVPQYQALRFIAGIGLAGELGAGITLVAELLPQRLRSYGAVIVSTMGVLGGILAFL
                     VADLFEWRTSYFVGGGMGLVLLVLRIRVFESGLFIKLKEKKVARGDLLMILTNGKRLL
                     KYVMGILVGIPMWFVVGVLMTFSPEFGNAHGLSGINAGKAVMLLFGGQAFGNMVSGLV
                     SQYFQSRKIGIGIFMIGSFLTMLAYMLLPVKTEFQFYGLILVLGFFNGYWTLFIIQAA
                     ELFGTNIRATVATTIPNFVRGTAVILTTAFAALRPGMGIISGGVLLASASVFIALIAL
                     FLLQETYHKDMNYEE"
     gene            5288..7120
                     /locus_tag="PKM27_03570"
     CDS             5288..7120
                     /locus_tag="PKM27_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNR06367.1"
                     /translation="MNKSLCFLIFLFAAQACKTVQDDKSTPMIRKDIHSYAEDAVIKQ
                     LILDLEADFENRVLKGSGTYQIETNGADYIVLDLRGPEIFTVLVDGEKADFKLGTEDP
                     ILGQALVIPVKPGSKTVSIFYQTRPDADALQWLSPQQTSGHQYPFLFSQGQAILTRTW
                     IPIQDSPGIRFTYQATIRVPKELMAVMSARNVQVKNPDGIYRFTMLQPVPAYLMALAV
                     GDLAFKATGARSGVYAEPSVLEKAAWEFGDTEAMIAAAEQLYGPYAWERYDLLVLPPS
                     FPFGGMENPRLTFATPTVIAGDRSLVSLVAHELAHSWSGNLVTNADWNDFWINEGFTT
                     YFERRIMEALYGREYSEMLASLALAELHRLVLEIGPQSKDSHLHLDLKGRNPDDGMNS
                     IAYEKGYFFIRLLEEKIGRSRFDEFLKKYFSEHAFQTMNSERLLEYMKRHLPLDSLQI
                     NAEEWIYHPGIPANCPAPVSDRFDKVEQAYRQMAASQHWDTLLTSGWSSHEWVHFIAQ
                     VPDTVHPAQLKKMDQAYRFSESGNGEILDIWFEKAIRGGYSPEILERIEDFLVHIGRR
                     KYLMPLYKAFKATGQIETARRIFNRARPNYHSVSANSVEQLLKE"
     gene            complement(7207..7539)
                     /locus_tag="PKM27_03575"
     CDS             complement(7207..7539)
                     /locus_tag="PKM27_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06368.1"
                     /translation="MKKIKPMILLAVTFIFGGILESQAQTEIPKAVNELLVKHTCFTC
                     HKADVKLIGPSWMEIASKGLKKAELVQAVYAPDQSRWPGYPPMMPMPQVPKKDVEKIA
                     DWLVKLKK"
     gene            complement(7707..8387)
                     /locus_tag="PKM27_03580"
     CDS             complement(7707..8387)
                     /locus_tag="PKM27_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNR06369.1"
                     /translation="MKLTYYGHSCFSVKIKQKILLFDPFITGNELAGHIDISKITADY
                     ILLSHGHSDHTADAVAIAQATLATVVGAFEVAMWMRDQGVTQFHPMNTGGKWTFDFGT
                     VKCTHAVHSSSMPDGSYGGSPMGFIITAEEGTFYYSGDTALCLDMQWFRSSGPFRFVI
                     LPIGDNFTMGVEDAVQAAKWLGAEKVVGVHYDTFGYIKIDKGAAVAAFQKEGIDLLLP
                     GIGESIEL"
     gene            8465..8998
                     /locus_tag="PKM27_03585"
     CDS             8465..8998
                     /locus_tag="PKM27_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HNR06370.1"
                     /translation="MTDLEALKFPVGKFHKPDFVSPHHLKAWIQDIRDFPGLLAKELE
                     GLSPADLLRTYRPGGWTISQVVHHVADSHMNSFIRFKLSLTEEHPTIKPYLEGLWAEL
                     PDGKDLPPVISLNLLTALHDRWCHLLDRMTPKDFHKGYFHPEKNRVVNLDEATCLYAW
                     HCRHHLAHIRLAKQKAS"
     gene            8998..9405
                     /locus_tag="PKM27_03590"
     CDS             8998..9405
                     /locus_tag="PKM27_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNR06371.1"
                     /translation="MAWRLLFLIFFTWSAACKPSVSGSAPPGKKDSRTLNVQDFLEAY
                     RKEPAGQAMLIDLRTPPEFEDGFIPGAVMINYLEDDIDLQLGRLDKSKTYYIYCQQEG
                     RSVLCIQKMENMGFKKVYLMKGGYDAFLLSGPN"
     gene            9478..10236
                     /locus_tag="PKM27_03595"
     CDS             9478..10236
                     /locus_tag="PKM27_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNR06372.1"
                     /translation="MKSLLTWMAFLLVSPAFGQGNQGLITFEQKINLHRRIPAENEDL
                     KSRMPEFQTTLHELLFKENESLFRPLEEEEDAPMSGPGNGGMIMRFRRPETIIYRNHD
                     AEYQLAQRSLFEKKYLVEDTLRIAPWKLGMESKEILGYPCLKATLETETKRDTQWIRT
                     RVTAWFTQDIFSPFGPDTYGGLPGMILLLDINEGETVYSATRIDPSADLGDLKAPVKG
                     EKISGTQFKALMEERMKEFQRNGRGPGQRMIRMN"
     gene            complement(10299..10832)
                     /locus_tag="PKM27_03600"
     CDS             complement(10299..10832)
                     /locus_tag="PKM27_03600"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNR06373.1"
                     /translation="MNKSKKVSESKSVMTEMIMPNDTNALGNAMGGNLMRWMDIAAAI
                     CANRHAETYCVTLSVDHLSFRKPIPLGHVITLEACITRAFNTSMEIYVQVFTSDSRRR
                     DAELSTHAFFTFVALDADTHVPVQAPELIPETELEKQLYEGAMLRREMRLLLSGRLDG
                     KNARVLKEVIEGKKIFF"
     gene            complement(10864..11574)
                     /gene="lipB"
                     /locus_tag="PKM27_03605"
     CDS             complement(10864..11574)
                     /gene="lipB"
                     /locus_tag="PKM27_03605"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNR06374.1"
                     /translation="MAVPLEKQVILEDWGLVPYREAWERQTALHQSIIQKKTGPLKQK
                     ETGPFYLIFCQHPPVFTLGRSGKEQNLLINEELLGNKGIEFYRINRGGDITYHGPGQW
                     VVYPILDMEWFYTDVHRYVRSLENIVMLTLAEYGLEAFRNPKYTGVWLNSRHGGLPKK
                     ICAIGVHMSRWVTLHGLAFNILADLTPFDYIVPCGIRDPDKGVTSLHLETNRPPDLTQ
                     VKARLCYHFEQEFQCALV"
     gene            11715..12080
                     /locus_tag="PKM27_03610"
     CDS             11715..12080
                     /locus_tag="PKM27_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06375.1"
                     /translation="MKWSLTLVLFLICGLSRAQSKSDLNQHKSFEVIYTSLKVYPNPV
                     ASLATLEFKLAQPAQVRVSLLNILGAEVREILRESLPQGNQNIRFSADQLEKGTYFLR
                     LSVNGDIVKTIRVAVSNRT"
     gene            12143..13249
                     /locus_tag="PKM27_03615"
     CDS             12143..13249
                     /locus_tag="PKM27_03615"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNR06376.1"
                     /translation="MKICLIIWFTAICFLPCFGQKKAKDFKVTTTDLKTIELYKDYLN
                     KGKVVMVQIFFVDCPPCNEIAPNISALYKKYGSGSKRVEFIELSNKSWDNNAAVQGYK
                     TRYNLNFPSVSSEGGSVQAAALYSDNSYGSFLGTPTFIIIDPTGGVTFDPRGQHADAT
                     TVRLDTALARALRVPTVPGSGSGGEGNNPSTGIKRDTLNLHGKLSLGSSTLGLVTLKL
                     SWNKKSYTFRTDIQGSFKFQAIDTARTTNDIRMEVEYPLDYITSVSTIDLILIQKHIL
                     GVKPFGDYKQVIAADADLNGEINVVDLVELRKLILGLQDKLTAPSSQVTWINHRNLIR
                     NLSQAIPLEDIKSLGSNGIQLEIVKTGDVSLYNQ"
     gene            complement(13426..15183)
                     /locus_tag="PKM27_03620"
     CDS             complement(13426..15183)
                     /locus_tag="PKM27_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06377.1"
                     /translation="MKIQTKPLLTLSSLLLLGAWMLIQTSCKKEDEVFPAPVVTPGTA
                     ASGLAGTVVNITVSLQSERGLKTLQVLKNGADFDYKTYSEGTKTDQYSKTYTIEALPS
                     GTNINFTFVVSDYDDQVTVGGPQVITVSALPVKPIVDVSGILEGNVSWTKDKVYRLKG
                     FVRVGEDLTKDGNPTKTGVLTIQAGTVIIGERATKGTMIVHRGSRIIAEGTAAEPIIF
                     TSERAIGEREPGDWGGLVLCGKAKNNLPGGTGELEGQYGAFHGGNDDEDNSGVLKYVR
                     IEYAGIPINPNQEVNSLTMGSVGRGTKIEYVQCSYGLDDAFEWFGGTVNAKYLVAYRG
                     LDDDFDVDNGYSGNVQFAIGLRGATQADQSGSNGFEVDNDGSGSAAMPFTAPYFSNVS
                     IIGPKAEVATSISPQFQNGMHLRRNTKIKIYNTVVTGYPNGIYIDGSTTQANAANGEL
                     QLKNVVVAGLDGWGDNGFGNGTNHNPRGYAIRDLNTANPPADIKIGDKKPSEWFAGIE
                     GNKILLKRSQTGLQADIFTAKPTLVLTTGLSDGLEKGGSNTGLPAFFENVDYIGALKS
                     TNWTNGWSEFNPSAKDYMK"
     gene            complement(15209..17995)
                     /locus_tag="PKM27_03625"
     CDS             complement(15209..17995)
                     /locus_tag="PKM27_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06378.1"
                     /translation="MKQTFFTLLILVSGFIRLNAQNGTIQGIITDQKLSEPLIGATVK
                     VIGTELGSATDIEGFFSIDHVPAGEYTLQISYVSFRTENLAGVKVEAGRITQINLDLV
                     EETETLQEVVVTGDKVRNSEVAILSEIKASMQVVSGISEQQIKKSMDGNAAQIVRRVP
                     GITIVGDRFINIRGLNSRYNNVMLHNAFTPSMETDIKSFSFDIIPAGQIERMLVFKSP
                     SADLPGEFAGGLVKIFTKGIPSKSGLSVEYAAGFRQGTTFNDFYQPQLGKNYWTGFNS
                     GTFDLPKNFPASVNEVINNPIQLELAGKSLKNNWVATPTVAVPDQKINLTNNLRTVFG
                     GVQVGNITSVTYQDSKTQFGINRGDYNEQANGIESRIFSYDDAQYNRNIRIGVLHNWA
                     AKWDNHQIEWKNLFNQNSTGSYVYRSGKNFESNYIPNNHSFDQVYKNILSSQLVGTHQ
                     FLQDESLNLEWTFGYNRATRNQPDYRRYRSDFDESSGTSSLYVPFGAAQAFFLGRFSS
                     EMLEKTYSGNLAVSKKINDLEIKAGGALEDKNRTFDARNLGYVRGSAFDFNNDLINGD
                     ITELFNNINNRTGVKIDEQTNPSDSYRSSNKLYAGFVHVLTPLGKKLSFSGGLRLEHN
                     TQNLESALIGGTPVALHYPITKWLPSATMSYYFSQKSILKASYGMSLNRPEFRELAPF
                     AYYDFDYNYVYKGSPLKTAVVQNFDTRFEYYPTPFEVFNVALFYKKFKDPIESLVVPG
                     SGSGGAKTFTFANAESSVNYGVEVELKKSLSNLTPSRFLGRFSTVFNATLIHSAVRLG
                     DGISEGQSDQRPLQGQSPFIVNAALNYLDADQGLQVNVLYNVIGKRIFAVGFQGYPDL
                     YEMPRNVLDLMISKELGSRIILKAGVSDLLNQPARILQDGNQDKKWDPSRDQIIQQYA
                     PGRLVQAGLTYQIY"
     gene            complement(18269..19162)
                     /locus_tag="PKM27_03630"
     CDS             complement(18269..19162)
                     /locus_tag="PKM27_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06379.1"
                     /translation="MRLKGTCSILFVALCSFIGLNGHAQSGNLSGTDLLRIQNYEDTI
                     AVWARGVLNAEIEDERIYACHQMIPTLAKALKVDHSFQYKFPRLENVAITYPSDSSFR
                     IFSWQLKVNEADYRYFGALQWNSPSLKLTSFADRSHELADPESVSLPADRWYGLVYYN
                     MVPFEMADKSRAWLLFGYDGHSVYERRKVIDVLHFREGKPVFGAPVFRMGKNEIRHRI
                     IYQYSADAAVRVNYDENEKMIICDHLMEQPGNLPGQGRTRVPEGSYEGFRLDKGIWKY
                     VEEVFPSGNAENQRALRSKKN"
     gene            complement(19215..20147)
                     /gene="mdh"
                     /locus_tag="PKM27_03635"
     CDS             complement(19215..20147)
                     /gene="mdh"
                     /locus_tag="PKM27_03635"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNR06380.1"
                     /translation="MSKVTVIGAGNVGATCANVLAHQDIVNEIVMLDIAGDLAKGKAL
                     DTWQQAPIDDYSTRVIGTSDYADTAGSDIVILTAGVPRKPGMSRDDLITINAKIVSGV
                     TKSILEHSKDPILIVVSNPLDVMTYAAQKTSGLPTAKVIGMAGILDTARYRAFLADAL
                     QVSPKDIQAVLLGGHGDTMVPLPRYTTVSGIPVTELIDTETLNKIMERTKVGGGELVK
                     LMGTSAWYAPGAAAAQMATAILKDENRIFPCCIKMEGQHGLRDVYVGVPVKLGAGGVK
                     QVIELKLNEDEMALLNASAQAVKSVMDAYDALGL"
     gene            20273..21772
                     /locus_tag="PKM27_03640"
     CDS             20273..21772
                     /locus_tag="PKM27_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06381.1"
                     /translation="MRWFLIAGSLLFFSAVKAQEDKPFLCGTENLEEIKQVMLKNREE
                     FKNFQFQRGAISYIPVKIFLVAYNDGSGQMPVLNALRMLCQLNTAYADMELQFYIKEI
                     KTLNNSTITETPRSSGGTLQMQLNKDPKAVNIFITHKILDGVAGYYIGPAGSNNDFIV
                     IQKDYMGDVRVAAHEVGHYLSLPHTFHGWDSEAWDPVKHGNPVQKFAPDGITINEYAD
                     SSNCGPKNVGDGFCDTPADYNFGANTCSYSLKAMDPNGKLVSPQTNNMMNYFFGCSKY
                     LFTPQQKAAILSSYNSTSRRDIRGTSPSEVTPINQAVKLLKPADKSATASADSVLLEW
                     EAVAGAASYLVQYDIVPTFEVFVQTFITKETKQKITGLVLNRTYYWRVIPFNDYSTCY
                     PDPPRFSFKAGALTSVRDIPEVKSLSIYPNPVANQQLLNVYLDNHTGFSGSISIFDLN
                     GRLVLQVPDQKFTAGASSKGLDISGLRPGVYAFVLSSRGGLASRRLTVF"
     gene            complement(21783..22259)
                     /locus_tag="PKM27_03645"
     CDS             complement(21783..22259)
                     /locus_tag="PKM27_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNR06382.1"
                     /translation="MQVQTTKMVHSKWMMFWYFLLHLPGAFFFGLKVVSLTEERAQVN
                     IPYRWSTKNPFKSIYFAALAAAAELSTGIIALTSLDPSRISMLVTRVEGQFLKKAKGP
                     VLFECNQVQDMRKTAAIALEQDAGVKIIMNSTGRNREGEVVAIFSFEWSLRKKQAG"
     gene            22316..23524
                     /gene="sucC"
                     /locus_tag="PKM27_03650"
     CDS             22316..23524
                     /gene="sucC"
                     /locus_tag="PKM27_03650"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNR06383.1"
                     /translation="MNLHEYQGKELLKKFGIPVQEGFLATTPEEAIKAYDQVVESSKS
                     PVAVIKAQIHAGGRGKGGGVKLVKSREQAAQTAPQILGMMLKTPQTPGGMNGPGKLVR
                     KILVTEDSFKPDFESCKEYYVSILTDREKKANVIIYSTEGGMDIEAVAEKTPHLVHKE
                     YVHPLAGFQAFQARNIAFKLGLSGESFKNMVRFVTNLYKAFTESDATLFEINPCLKDG
                     YGNILAVDCKVSLDDNALFRHSDLEAMRDTDEEDPAEVEAKAYNLNYVSLDGNVGCMV
                     NGAGLAMATMDIIKLSGGQPANFLDVGGTADAARVEQAFRIILKDPAVKAILINIFGG
                     IVRCDRVAQGVVDAYKNIGEIKVPIIVRLQGTNAELAKDIIDQSGLRVRSAILLKDAA
                     ELVREALLEK"
     gene            23540..24766
                     /gene="xseA"
                     /locus_tag="PKM27_03655"
     CDS             23540..24766
                     /gene="xseA"
                     /locus_tag="PKM27_03655"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNR06384.1"
                     /translation="MTRISLFELNEYVRQVIALNFTEPLWIRAEINQVSFSRGHCYLE
                     LIEKKGDTDEIIAQASAAIWYRNLRFIEQKTGPNLNQLLQKGIQTLLKVRVEFNERYG
                     LKLSVEDIDTTFTIGQLELKRREILQQLGALGLMDKNKGHALPPVLQRLAVISSEKAA
                     GFQDFIQHLQANDYGFAFECELYSAAMQGRNVEKEILAAFQEIRQARQPYDAVIIIRG
                     GGSRLDLAAFDSLQLCVAIANLEYPVITGIGHETDQAVADLVAHTSLKTPTAVAHFVL
                     EHNFKFETQLYAGYRILQKSALDIIESFQSRLRRQSQMIKDISLRRVGNENYFMAAAK
                     KDLKMYTLQLLREKQAELHNLQQRTALMDPELLLKRGYSMTFKDGVLLKSPDQAESGD
                     LIETRLSGGSIRSTVK"
     gene            24788..24994
                     /gene="xseB"
                     /locus_tag="PKM27_03660"
     CDS             24788..24994
                     /gene="xseB"
                     /locus_tag="PKM27_03660"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNR06385.1"
                     /translation="MSKTRKDKGSFEEAKRALDEIIAELQNENTSIDKLASQVKRAQE
                     LIQYCQSRLRTIEKEVHSLLDEEE"
     gene            complement(24967..26115)
                     /locus_tag="PKM27_03665"
     CDS             complement(24967..26115)
                     /locus_tag="PKM27_03665"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNR06386.1"
                     /translation="MKILLALNSFKGSCSSLEANNALRKGLAGTLNPDSLTTFPLCDG
                     GDGSVLALAHHLHLKPVWITASDALGHELKAPYLFEQESSTAYIEMAAASGLAVLNGR
                     EKNIWISNTMGTGELVQHALKRKARRVILMVGGSATNDAGLGIMHRLGIRFLDKKGRV
                     LDPVPANLIQIHAVDTRMEMEGSRTIEIWTDVTNPFYGPEGAVEVYGPQKGAGPPIRK
                     KLEKGMIHFARVIHNQFGTDLQKVKGSGAAGGIGGGMHGIMGCTIHHGTSRIFELLRF
                     REKVQAHDLVITGEGALDFQSRFGKLVHGVLKTAGKCGKPVIGLCGSLQMNTAQNRKM
                     GFSAAFSIVRGPEDLETAIKMGKENLEAFGANLAALIRVVYSSSSRRE"
     gene            complement(26112..27104)
                     /locus_tag="PKM27_03670"
     CDS             complement(26112..27104)
                     /locus_tag="PKM27_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06387.1"
                     /translation="MFKWGIIGLGRIANKFAKELKETGVGELAAVGSRDITKAEAFAK
                     EYGAARAFGSYEELVGHGGLDAVYVATPHHLHAYCTSKCLEAGLPVLCEKPFTINKHE
                     LTGLIHQARVHQVFLMEAMWTRFLPHILYLKSLHDSGRLGRLLHLRADFAFKGKERAS
                     AQGVSRLVVNELGGGSLLDIGVYPVFLAQLLLGVPAVIQAEATILNEIDETCQVILKD
                     AEGITAYLESSILWESDTSAQLFYEKGSVLLPRRWHERGEMIIKPENGEAEIKNWDYP
                     NRGFYYQIKEVQDCVQAGKLESDLMPLHFSLQIMDTLDRIRTLIGLKYPADLMK"
     gene            27264..28496
                     /locus_tag="PKM27_03675"
     CDS             27264..28496
                     /locus_tag="PKM27_03675"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR06388.1"
                     /translation="MAINLLELLQSQVGGELVGQASKFLGETEVNTGKAVGSIFPTLL
                     GSLVGKASTESGSRGILDFIKTNNIDGSVLNSLGGLFSGGQATDSLLGSGGGILKFLV
                     GDKLGSIVDVISNVSGVKTGTSSSLLKMAAPLLMGFVGKFVKEKALDAMGLKNLLGGQ
                     REFISKGIPGALTGLLGLATNAGGGAPKMPAGPSSTTGSSNLLKWALPILLLLAVIYF
                     FGRKGCDRQMETTPTEMVDSAKLAAEAAAAEQKRIADSIAASRSVYTLPGGISLNTKK
                     GSFTDKFVSFFADSLAKVDPKMAFTFDGVHFKTGSDSLTMESGTQLDELASVMTAYSG
                     VNIRVVGHTDNVGDAAMNKKLSDARAKSVKAYLVGKGIAGRRIETLGMGQASPIGDNN
                     TEEGKAMNRRVEVFVTRK"
     gene            28672..29904
                     /locus_tag="PKM27_03680"
     CDS             28672..29904
                     /locus_tag="PKM27_03680"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNR06389.1"
                     /translation="MLAIVFLVFLNSSIEAQSVLNRFQVIGRPKQIDIPFQFENNFIL
                     VKSLFNRSFPLNFIFDTGAEHALLFKREYADILGLKYDRRIRLIGADQSEEMYALIAR
                     GVSFGLSPTVSYSQDILVLEQDFFHLDEVTGLYVDGIMSSSFFKGYIQHIDYRKRILS
                     FIQPSVFRYNSRKYTKVPVEIINGKLYVTGQVEIQNKKIPVKLLVDSGAGLPLLLYTN
                     ANPDFAPPEKAIPGRLGKGLGGYLEGHIGKITELQLEKFKFPNIVTSFQQLDSTLIPA
                     GAEETRFTRDGLLGNQLLSRFDIYIDYNKNLMYLSPIKDLSKEFLVDRSGMEILATGH
                     ELKQYIVNSVIPGSPADEAGLKKGDIIKRIGFLSTNILSLEQIIHSLARKPGKKVRIQ
                     YFRNATEYKTTVTLRSLI"
     gene            29970..31802
                     /locus_tag="PKM27_03685"
     CDS             29970..31802
                     /locus_tag="PKM27_03685"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNR06390.1"
                     /translation="MYSSLKACVEDLERQGQLFRVKEEVDPDLEMAEIHRRVFDSGGP
                     ALLFEKIKGCRFPAVSNLYGTRDRVLYLFRNSLEDLKALGRLKNDPGLILKKPFSYLP
                     AVLKARFALPRKIRFSRSMVNTCSISDLPQIRCWPMDGGPFVLLPQVLSLPPGSRDLL
                     QSNLGMYRIQLGGNAYLPDQEIGLHYQLHRGIGIHHKMYLESDEPFRVSVFIGGPPSH
                     SLSAIFPLPEDMSELTFAGLLNRKRFSWFLKDGYVLSDQADFVITGTIDKSLKKPEGP
                     FGDHLGYYSLTHDFPVMKVDKVYHRKDALWHFTVVGRPPQEDSFFGFLIHEIVKELIP
                     REFPGLTGLHAVDVAGVHPLLLATGKERYMPFREPRPEEILTIGSRILGSGQTSLAKY
                     LFIAAPVEREKLDLYDLPGFFDYILRRIRFDRDLHFITNTTIDTLDYSGEGWNAGSKV
                     MVACCGPVIRDLSERLPEHLVFPPGFMRPVLIQAGIVCVEGTRFTDHSKESQALDQWT
                     ASQEPDNWRGIALIVLCDDSAFTASNWDNFTWVTFTRSNPSHDMYGIGSFTSFKHWGC
                     RGPLIIDARIKPHHAPVLEADPATTAKVDRKFSKGGSLYALHRK"
     gene            31809..33176
                     /locus_tag="PKM27_03690"
     CDS             31809..33176
                     /locus_tag="PKM27_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06391.1"
                     /translation="MKPSTPYPLPDAFLKRMQDQLGTSYSSFLDAVEKISPPAVRLHP
                     LKLAGHYEFPLDLNIPWEDHGYYLRERIKFTLDPSFHGGAYYVQEPSSMLVGPLVKRL
                     LESYDQPLVLDLCASPGGKSTAILSALNGRGLLLANETIAGRISGLQHNLIKWGYANA
                     LISQSDPIQFQAFPGMFDLILVDAPCSGEGLFRKDHSSRKEWTPDLALQCSLRQKRIL
                     RDILPALKPGGILLYSTCTFNPAENMEQLHMLGGLGFESIPLKEWDDFPLVRLEENGV
                     TGYQAYPHLMNGEGFFVGALRKGTGSQGPVPTTAPTGVEWVNPPRESQRFIPSGSGLK
                     CFLHRDQYFVFPSAMADALDQFIGRLRLVQAGTALGQLKNKDFVPAHGWAQSLDCPGT
                     VISLNVEKAEALQYLRKDPLPRQGLDPGYHLIRYEGISLGWIKSLPGRINNLYPMAYR
                     IMQAG"
     gene            33242..34744
                     /locus_tag="PKM27_03695"
     CDS             33242..34744
                     /locus_tag="PKM27_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNR06392.1"
                     /translation="MKLFSGAQIREWDKTTLEEEGIPSIELMERASSAFTKKFMEHYD
                     RSRPIAVFCGPGNNGGDGLAIARMLNTQGYEVKVWLADWGLPKSPDYEIMLKRLPGFG
                     QVPVYSVDMKEPPILGEDDLIIDALLGTGLNRPLDGNFEDLIYWLNSLHKTVVSVDLP
                     SGMFTDQHTELAIRASHTITFQTPKMAMLLPEYEEYTGSWEIVDIGLSHSYYLKTTVK
                     HYLVEKKMLKGFLTTRKKFSHKGLFGHALLINGSHGKGGAAILAAKACLRSGVGLVTN
                     HIPGALYAIIQMAVPEAMASVDEHNYYWSTLPKDLDKYAAIGLGCGLDQKDSTARVLS
                     QLFDEYDGRMVIDADALNLISKSKDLLGKLNPGSILTPHLKEFERLFGKSINCFERLS
                     LLSEKAKQYQVTIILKGAFSSVANPEGTIFFNPSGNPGMATAGSGDVLTGLLTGLVAQ
                     GYDSTQAAILGTYIHGAAGDLALKKQGGESLVASDIIKRLGEAFKDLRAA"
     gene            34741..35430
                     /locus_tag="PKM27_03700"
     CDS             34741..35430
                     /locus_tag="PKM27_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNR06393.1"
                     /translation="MKKLVVLTGAGVSAESGLKTFRDADGLWEGHRVEEVATPDAWER
                     NPSLVLQFYNERRQQLSKALPNLAHRLLAELEKHFKVSIVTQNVDDLHERAGSSQVIH
                     LHGELKKMRSERYSHLVYDCPGDILLGDLCEKGFQLRPHIVWFGESVPLLALAVPEFE
                     SADICIIVGTSLQVYPAAGLIGYLPTSCRTYYIDPRPHPSYEIGLLNNISLISKKAQE
                     GMKEVFDRITS"
     gene            complement(35545..43092)
                     /locus_tag="PKM27_03705"
     CDS             complement(35545..43092)
                     /locus_tag="PKM27_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06394.1"
                     /translation="MIIRLTNFVRRPFISVRLLSLILTGYFTFKVLSKPDLISNPPLQ
                     LSPYSFEEAPTLLNAGMGGDTITDVRVEGDDIEQGACNLAAPWLMAGNTTASDDAYAT
                     NSRVLAIGSAVSNCLDLQFLGFSIPSGAEILGITVTVERSANVRTATNNVTDNKITLI
                     RGASNTEAQNKALSITWSTTDSTITYGSSSDSWAYPSWTPADINSPLFGVRIQNNRNR
                     ASGTITARVDYVSIAVTYVTPKKSSVASTVGFGCPSSDGSASWTGATAANLASDDGLI
                     VLVPSIYTGYATVTNNIGDAVDTSACLDLTDFGFCICPNAIIDSITVEVDRARGFDIL
                     QQVVGQDTVTDVKIALLQPGGAESANKANTATNWPGSTASVVNLITYFTGDQRVSYGG
                     DNWGVNWTPAMINDPGFGVRIQVRLADNAPILPPLASARIDEVLVKIHCSFPRKIYVD
                     QTATGANTGLSWEDAFTDLQVALNACFVDTICVAAGTYYPAVTGRDTSFNIPDSAVVM
                     GGFPAGGNGERDPWCYKTILSGEIQQDDDSTNNSYHVVTTHNVSDATVVDGFCIQDGY
                     ASAPPNEYNRYGGGWFNFATAPDSSNPTILNCTITGNAALRGGGMANFGDMGPTLPTL
                     INCIISDNEASRGGGMFNYGLNYESSPTLINCILSGNLATDFGGAMYNDAEGNGDDNT
                     ATSHPMVINSTFNGNQARQGGATFNNGASGGDLNLFIRNSAFWNNIATQPNRGPVFWI
                     QDGTLDIDYSLIQGGGGPPGDLTHSSITRDNGGNTFTIMTGPNLKFGSEGFDPLFFNA
                     PLGNTAPTPVGNFRFPYNSPLVNMGNNADNPYPTDIAWLPRIAQGTIDIGPYEAYLNC
                     LNDTIYVNADGTVPVLPLDSVLLGELPDGTTFLVNGIETTGDIVLDCEQLGKNNLLLE
                     IVRNCLVDSCTKMVWVLDTFTKDLVCNDLVNVSLGTSCMKVIDPDDVLEDVLGCRNYW
                     DIQLIYPFGTARYPEADKVDLSHIGYTLVYNAYEVSTGNKCWGYIHVEDKQPPQLNCS
                     VDTITCYELPDLPLLAEGEDSCSGVEVKIVSDHWTEYGCDEDPVLSGYVVRTIQGSDK
                     WGNFARCTDTIFIRKTSLDSLVCPELIELPCEITHQLYGSKSELKTPFTVDKSKVSPE
                     YLLSLQKANWELKAGGRGAILDPAIIVVPQIEGKNIYPAAGGSCKITAIYKDTDLDVC
                     GAGFKVYRNWIVTDWCTLEEEECEQYIKVIDTTGPRMLKSLATIYKNTNAHECVAGVV
                     VPALVAGTDYKDCSGVKQSYRITYEDKSHPGKVIVLAGDLPSKTLYLPQGEHMVQIFL
                     VDDCYNRTETSRLIVIKDITPPTPVCDEVTQVTVDPEGCWAKVKAKDLDNGSRDNCCE
                     VLHFAVAHMDSVTYWRNYWRDSLEARCGSYKYEKDKEFYEFQIERWINAYVFKDEIKF
                     DDCGPNQVVLRVYEACGVPIYDPHVWPCTEHQWFWYNTDQRYRIEHNWNFFHKDGKKD
                     CNYRYKIECQKEHQQRGREICEFGNNTYPKEGGLCYAQYEGAVENLNAFCESDFYFEE
                     TVMLTGGTANPPGSYCSHRLYSDCMVQVLVDDKQAPEVHHLEDITVYCDDAPYYAGYP
                     KDCSTGKKNGIWPGYLKDSEGTIHGYYGGLGYYDEEEHGSDCGHYDHQNNWAPKYCRE
                     WLYLDSFDTSGQIDPKSYFDTYVLFDKNRPQRELKKNEFSITENCTLNDATLTWTDEG
                     DINSCGEGWIQRTWTIRDKCDNVVVAKQKVLVKHRSDFEVVFPEDKVVECDFLNSTEP
                     EDTGEPEVTDDECEQVGIRYKDEIFTIEGDACYKIVRTWTLIDWCIYDPGRQERYPEV
                     IVDDRLRADTGNRECVYRNLKDNNDGYMQYIQIIKVVDKVAPIVNTRDTIVCVYNEDC
                     SADVIIPLSASDNCADPSNIRFRVQIDIDASEDAYNGRLYDANSIDQGGTRYETEFIF
                     LSGTPGKHLVHIIGIDNCGNADTSTFRFELRDCKRPTPYCYRGLATVIMPSTGTVTVW
                     AKDLDAGSGDNCTEYDNLVFSFSSDITQTSRVFTCADLPDGRSRTVPVEIWVTDEAGN
                     QDHCSTYILLQDNTGNVCPDVAGLNVQIAGSVQTEGKEPVEFVTMNINGGGLQLNYET
                     GVQGTYHFEALPSKSDYTIRAKRKDRPMNGVSTLDLILIQKHILGTEPLDSPYKIIAA
                     DADDDKQVTAIDLVELRKLILSTYEELPNADSWKFVPKTQQFSDPKNPWDFKTWMEMK
                     EVEKDYMQEDWIGIKTGDVNASAAPHSLMGTEVRGNNPGLIFDVVDQQFIAGELVIVE
                     FRSPNFRGISGFQGTVEVEGGRWVVDGSRTPKTGALPMSDQNFGNRKAAEGLITMSWN
                     SNTGVDIPDQEVLFTLTFKAGQNGRLSEALRMGSQVTIAESYEGKGEVSNLGIRFVTP
                     EGNEVNARSELYQNYPNPFDQRTVIGLRLAKEGKGTLSIYDVTGRIIRSIERDWTKGY
                     HEVWFDRKEIGATGVLYYRFESGFFTDSKKMVIVE"
     gene            complement(43450..43821)
                     /locus_tag="PKM27_03710"
     CDS             complement(43450..43821)
                     /locus_tag="PKM27_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06395.1"
                     /translation="MHRSLGGPWKFFQSKERIWGRLFGSGFVTPEGTEVNAKSELFQN
                     YPNPFDQRTLIGFRLAKEGRGTLSIHDVTGRTLRSIEKDWTKGYHEVRFDRKEIGATG
                     VLYYRFESGFFTDSKKMVVLE"
     gene            complement(43778..49294)
                     /locus_tag="PKM27_03715"
     CDS             complement(43778..49294)
                     /locus_tag="PKM27_03715"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNR06396.1"
                     /translation="MNPSIRLLLLAGAPFFLLYCKQGPSPETSQESLGTVLRSYTQGI
                     ISSKSPLRYVFTQDMVEQEEVGSPVAEGMVKIIPAAPGKAYWESRNTLIYKPAGPLKP
                     GTRYEIKIDLKSILPRGMQGPEMLSYSVRTREHSFAVAKPMLQPASGELNFYTLTGTL
                     STSDFMEPGLVEKLLMASQDRQALRASWQHDTFSNVSTFTFHDIKRIKDSRLELNWSG
                     APADISKKGKYEIRIPAPGEFMILDVFFEKSPESRIVISFSDPLLANQNLTGLIYLKN
                     QAGPLRTMTEGHKIYAYLPRGLKGEQTVLISPGIQDSGRKKLQESSEWEIQIPATDPA
                     VRFTGRGTIIPQHDEVILPFEAVGLKAIDLEVFKIFDQNILQFLQVNQLEDEQELYRV
                     GKLIRQSRIDLATVNPRADPENWSHYALDLQPLLEADPNAIYQVRIGFQPSYTHLNCM
                     AGFEDKPGPDPGDELISFYEDNFYGPEGYYEGYEWEDRNDPCKAAYYNNEHFISKNVL
                     ISNLGIIGKKSDKHLFAAVTDLRTAEALAGCSIEVYNFTLEKIAQWTTDQNGLVNQLL
                     TEPPYALVATFQGQKGYLRMADGHNLSMSAFEVDGEDVKKGLKGLVFCERGVWRPGDS
                     IHFSFLLNQKEHLLPEGHPVSLQVFDAQNRKVIQKISSDHIQGFYSFPFKTEQDAITG
                     NWRAVVKAGGVEFNKILKIESIKPNRLKINWDVGEEITPSQQTAVLKAQWLTGLKASG
                     LKAMVTTQWRPSSGSWPAYKEFVFQDPARISNENPVITLFDGPLDEEGGTSIPLGISK
                     DFKPAGRMNLQFKIEVSEPGGDFSTFTHSALYHPYLQYAGIRLPQDPWGYRTLDKGKS
                     STIQFVSVDPAGKAQAGRKLTVGLYQMDWRWWWEEYGGSYAAYNSSDHLKAIQKTNLT
                     TNANGLAEWKVTVNQWGRYLIRVCDPVSGHCSGDFAYAGWPEDESRSFDMATLLRLQA
                     SKEKYLADEEVEINIPAPLGSRMLITIENGSQVLESHWANADKSPFIFRFKPKPEMSP
                     AVYVQVSLLQQHGDSGNDLPMRMYGVIPVYFDHPSTLLKPVINSSASLRPDRNESIEV
                     SETEGRPMAYTLDIVDEGLLDLTNHKTPDPHGHFFAKEALGVRTWDIYDQVLGAFGGR
                     IESILSIGGDEGGAKLNDSKVVSRFKSPVIHLGPFQLKKGEKKKHNINIDNYIGSVRV
                     MVVAAAEGAYGKAEKTLPVISPLMILPTLPRILSTGEQVTLPVNLFTSKDITQPITLT
                     AQDKNKRLKWDDPSKTLPAGGEGERLEYFSFNTDGRTGETTIRIEAKSGKESAKQDIE
                     IEIRNPNPMVSTVMNEVIEPGQSKTLAFQAQDAVTTQKASLEIATLQPVSLGKHLDYL
                     VRYPYGCAEQIISAAFPQLYLDQYVTLNENQSRELKINIESAITSLSRFSLQDGSFSL
                     WPGQAYASDPWVTSYAGHFLTEAEKKGYAVPQYLMSNWKAHQKKLAGLWDPAQKAFYK
                     ANHGLDQAYRLYTLALSGTPDLGAMNRLKEYKELGNNARWRLAAAYAVAGQTEAARTL
                     VQIPFVAEEYSESGFTYGSALRDLAMALETWLEIGDRTKAASMALDLNKQLNLEKPWN
                     TQSLAYALQALGKFHTGAASDGTTWSFKYSYDGGAKSNIHSRNASILIDPIRDGKMPG
                     SVTIENTSTQPIYLQSVQVGQPEQAPERPISNNLTMNVRYRSPERQAIDPGQLKKGDL
                     ILVELTVTHTGSRPRPFAHLALNHILPSGWEIINQRLSGMTAGGQEFDYQDIRDDRVF
                     TFFQLEPGKSKTFTLEARATYEGRFHLPAIVCEEMYDASVYAQVPGRTVEVLPK"
     gene            49718..50458
                     /locus_tag="PKM27_03720"
     CDS             49718..50458
                     /locus_tag="PKM27_03720"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNR06397.1"
                     /translation="MEANPKQIESLTGLLSGQEDLRLALQLACRTFSGRAVFSTSFSE
                     EDQIIFHYLNEIDHPASTFTLDTGRLFPETYSTWSRTLERYKLPIRSFHPDATALQAF
                     IESRGPNSFYESVENRKTCCHIRKVEPLKKALQGQALWITGIRAAHSPDRNDMTPVEW
                     DEANRIIKYHPLLHWTHEQVKACIAENQIPYNPLHDKGFVSIGCAPCTRAIRPGEDFR
                     AGRWWWETNKNKECGLHVHADPSKPSIT"
     gene            50455..51363
                     /gene="cysD"
                     /locus_tag="PKM27_03725"
     CDS             50455..51363
                     /gene="cysD"
                     /locus_tag="PKM27_03725"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNR06398.1"
                     /translation="MSDYRIDHLKELEAESIYIMRETAAQFERPALLFSGGKDSITMV
                     YLAIKAFSPAPIPFPLVHIDTGHNFQEALDYRDALAARTRSRLIVRKVEDTIREKNLT
                     EPRGKFASRNWLQTHTLLDTIEEFKFDACLGGARRDEEKARAKERIFSVRDEFGQWNP
                     KLQRPELWNIFNGRIHKGENVRVFPISNWTEIDVWNYIRQENIPLPTIYFSHEREVIS
                     HEGQLIAVSPFITLEESDTRLIKKVRYRTVGDMTCTAAVESQAKTLNEVIEEILSSRI
                     SERGETRIDDKQTEAAMEDRKINGYF"
     gene            51388..52656
                     /locus_tag="PKM27_03730"
     CDS             51388..52656
                     /locus_tag="PKM27_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR06399.1"
                     /translation="MELLRLITAGSVDDGKSTLIGRLLYDSKNIWIDQIEQLEKISKT
                     RKTTQIDLALLTDGLRAEREQGITIDVAYRYFSSPRRKFIIADAPGHIQYTRNMVTGA
                     SNSELMILLIDARQGVIEQTRRHSIIASLLKIPHIVVTINKMDLVDYSENRYLEIIRD
                     YQEVASRLGLPDVHFIPISALGGDNVVNHSSNMPWYEGPALLPLLEEIEIRRGDTLEQ
                     SRFQVQFVLRPQTDDLHDYRGYAGQVSGEAFKKGDAVRVLPQGLASRIAKLEHHGKEV
                     EQAEPGHPAVIHLEDDLDIGRGDLIVRAGEEPLVTKSHEAVICWMDEKPLVTGNKYLL
                     QQHSRLIKTAVREIAYRINVNTLDHETGIEKLELNQVAHIRLQTAGPLAYDTYEQSKA
                     TGSAILIDETSNRTVAAVLFLHPQAVDSTH"
     gene            52661..53482
                     /gene="cobA"
                     /locus_tag="PKM27_03735"
     CDS             52661..53482
                     /gene="cobA"
                     /locus_tag="PKM27_03735"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNR06400.1"
                     /translation="MDKPIPKIILAGAGPGDPDLITVKLAEALKTAEVILVDRLVNPE
                     ILKKYASGQASILYVGKQGYSGNSFPQHEINRLLTEYALAGKKVLRLKGGDVSVYSNA
                     FDEIQTLQNHSISYEIIPGITAASGAAASLGIPLTARNLSPGLRILSLSAGQEPGPGE
                     FNAWAKSPETLAFYMSIENLPSLINGLLEAGADPEMPLALIERATLENQNSAVYPLGR
                     FLTETANQSFASPTLVLIGRILNHIQPGSTPSSTGKEITKPESNPPTLNNFLHVI"
     gene            53472..55151
                     /locus_tag="PKM27_03740"
     CDS             53472..55151
                     /locus_tag="PKM27_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNR06401.1"
                     /translation="MLSEGRLRTLNDFIEKTTKEELIWINGYIAGLISSAGPSVAEPA
                     QPSKKFTILYGTDTGNSKKLALEFTALAKKQGGKVKVSGMDAYKPEDLAQEKQLCIIM
                     STHGDGDPPPAAKKFYDHLMETGTQYPNLEFALLALGDSSYPLFCKAGEEVHNRLLAL
                     GATSVFPLQKCDIDYQEEANNWFQLYLNSFPSAAPAPPVPPKKAPHTKKWYSGVIKTN
                     INLNGHTSSKETHHLEIRTLEPVLYEPGDALGVIPKNRKSTVDEILSLLQVDSNLSVP
                     YKGSAYTLSNLLTQTFNICHLKESQVQKYADLTGHEIPKVRMDLLDLLKIYPVRNSRQ
                     FIEFTAHLTEQLPRLYSICSSPQAREFEIHILVGKNSFEAQGENRFGLCSEQLNELYE
                     GTPVEFYIHKNKSFRLPSPDTDVILIGPGTGIAPMRAFIEDRDASGASGRNWLIFGEQ
                     HFVSDFYYQTEIQQYASTGILHQVDLAWSRDQKEKQYVQHRMREKGEELYEWLRGGAH
                     VFVSGTRSPMSEDVEKAWIDIIYTHSGKSAPESEAWWNEIKESGQYKADVY"
     gene            55173..56861
                     /locus_tag="PKM27_03745"
     CDS             55173..56861
                     /locus_tag="PKM27_03745"
                     /inference="COORDINATES: protein motif:HMM:NF010029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HNR06402.1"
                     /translation="MSNPKTGEPNLSQVEKIKMKSDGLRGSLAESLQDQLTGALREED
                     QSLIKHHGMYQQDDRDLRESRAQKKLEWLYSYMIRLRLPGGFLNPSQWVALHHIAGEH
                     STCVIKITTRQTIQLHGILKSHLKPTLKAFATQRLDSIAACGDVNRNVICSAHPKQSR
                     AHEEVFAYADKISTLALPRTNAYYEIWLDREALTNKEEEIDPLYQDRYLPRKFKIAIC
                     IPPNNDVDVFANDIGLIAILDGDKLLGFNVAIGGGLGTTHGNADTYPRLATVIGFVAS
                     EEKILKAVYEIITVQRDFGNRSDRKQARLKYTIDRMGLDAFKSEVEKRCGFEFEPAKP
                     FSFSERTDYFGWHQNHEGNWYYTLFVENGRIADENRAIKTALYEAAQTGKTNFRFTAN
                     QKVILSDIHPSDKKAIEQILEKYDLIKHTDEAIPMRQNAIACVALNTCPLALAEGQRY
                     LPTLISKLEPLLKKYKLENDPISIRMTGCPNGCGRPYLAEIGLIGTSYGRYNLHLGGD
                     RLGERLNEKKYEQVNEEEIISILDPLFKKYAAAKKKYQSFGDFIHQNPELVTTR"
     gene            56867..59626
                     /gene="hemA"
                     /locus_tag="PKM27_03750"
     CDS             56867..59626
                     /gene="hemA"
                     /locus_tag="PKM27_03750"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HNR06403.1"
                     /translation="MPVNPSHLSDPLAPFDYQGWAENTLQGLKLEGAYRYFLGIEKRA
                     ETYPQFDFTDSSGQTKSAINWCGNDYLAMSTQEAVLKVMHKNIALAGAGSGGTRNIAG
                     TTRLHQELEQELASWNGKEAALLFNGAYQANQTTMTTLGRLLPDLVFISDEENHASLI
                     EGMRAVPNKKYIFRHNDLNHLEEILRALPPAAPRLLVFESVYSMSGTIAPVAEIVALA
                     KKYQSLTYIDEVHAAGLYGPEGSGLSEELGARAEIDFINGTLSKAVGVFGGYIAASAL
                     WVDLIRSFGPGFIFTTSLPPALCAAAAASIRYIREHPEIRLAFFENVHLLRSHLDHHA
                     IPYSGNQSHITHIRIGPEDLCKQRSETLLHSFGHYLQPINFPTVPKGAACLRLTITPR
                     HTEEHILRLTEALDQVLDRTIVLTGRGSKLSRLQIDIAKNKIQDAFPRLRVETKYKDS
                     LGDQLSDIPLDTQEGVDFFTRVFADDLQAGRADIAVHSLKDLSGDHFFGSNRFAVIDR
                     EETRDIAIFNGDVGERLRQGLPVRIGTCSFRRQLMAEQFLSKALPRTGPEIKIECLPI
                     RGNVDSRLRKLGTDSYDGIILALAGINRMLRDEGTRKEMQVLLQDKKWMILPLIECVP
                     APCQGAIVTEACPDNVLARQVFQRIMDPVLHADCSDEKYAARQYGSGCLQRFGVTTLR
                     AASEKWIYAAGLDEKGRTFRDWYGIASPVFPLDPASIITSDELGFKAFRKPVPVQDGS
                     PSSLVYFIAHRHALDGFTNQQALEQGRIWAAGTGTWYVLAQKGFWVEGCADSFGLESL
                     TGLFDSPAVGIRREEILILTNAESTNRWKARGWNALGTYEARYESSLIDKYKLKEARL
                     IFWNCFAHFDEVKDLAGKDLIHACLPGRTAEGLKKSGIHPILFPSLDSFNQWKKNYIQ
                     SIIAA"
     gene            59587..60585
                     /gene="hemB"
                     /locus_tag="PKM27_03755"
     CDS             59587..60585
                     /gene="hemB"
                     /locus_tag="PKM27_03755"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNR06404.1"
                     /translation="MEEKLYSIHHRRLRLGKRIRELVNPFRISHRNFIYPLFVDESLT
                     ARREIEHMNGICSETRETVFEAISSTLASGINKFLLFPIPAKKRKDHFDFSFAADLVA
                     ELKQSFGDGIWLATDICLCSYTDHGHCGILNEKGDQVLNHESVKVLGEYAYLLAKAGA
                     DCVAPSDMMDGRVGHIRQQLNAFALEQVTIMSYSAKFSSQWYGPFRDACHSAPDQSLG
                     IKDRKSYQLSPLSSRDAVQCALRDEAEGADILMVKPAALYTDVIWSVKQKTNLPIAAY
                     HVSGEYASVELMAAQGVVDRAKAHLEVWAALQRSGADIIISYAAPFAKSWIENYDF"
     gene            60669..62153
                     /locus_tag="PKM27_03760"
     CDS             60669..62153
                     /locus_tag="PKM27_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNR06405.1"
                     /translation="MAVTKQIERNVLYPVFLKLDDLNILVVGGGNVALEKLHSILSNS
                     PSARITLVAPRILDAVKTLTAQKTNCTLINRPFEDSDLDGRDLAFLTTDQPDLHRHIR
                     DLARQKKVLVNTADTPELCDFYLSSIVQKGQLKLAISTNGMSPTAAKRIKEMLKSALP
                     DSLDEIILNLNTIRKSLKGDFEYKTQTLNEITRIWVENKNDKAARRWKKMASISLLVF
                     ASMLIGYFLFSEINLRELSGRGADWVVSLDRSFYIMVLAGFLAQLVDGSLGMGYGVTS
                     AIVLLSSGISPAAISGSIHTAEIFASGASGYSHYRFGNVNMKLFKALVVPGVIGAILG
                     ALLLVFFGNSGFGWLRPLLATYTLFLGIKFFINAFQPPRPKRKFKFYRTLAGFGGFLD
                     SFGGGGWGPLVTSTLVSRGRTVKYVIGSVSLTEFFVTMASALTFFSLLGLSHWKTILG
                     LIIGGLLAAPLAALLIGKIPRKTAFILLGTIVVIWSIRILWGAF"
     gene            complement(62222..>62467)
                     /locus_tag="PKM27_03765"
     CDS             complement(62222..>62467)
                     /locus_tag="PKM27_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06406.1"
                     /translation="GWKWSASLKESSGTKSCEAPHYQYHVSGVLRILMDDGTTFDCKA
                     GDVSSLPSGHDAWVVGNEPVVVIDFQGMVDYAKMKSE"
BASE COUNT        15213 a        16580 c        15682 g        14992 t
ORIGIN      
        1 aaatccttcc ctatgcgcgg gaggctggcg taaaactggc catcgaaccg cttcacccga
       61 tgtacgcggc cgaccgttcc gccataaata ccctggccca ggctaatgaa atggcagcag
      121 ccattgattc gccctgggtg ggagtggctg tggatgtgta tcacctttgg tgggatcccg
      181 atctggaaac ggaaataaaa aaatgcgggc ggatgaataa gttgttcgct ttccacatct
      241 gcgactggaa aacaaatccg gaggacctgc tcaacgaccg gggactgatg ggggaaggct
      301 gcatcgacct gcccttgatc cgcacctgga ttgaagctgc aggttttaat ggttttatcg
      361 aagtggagat cttttcaaac cggtattggg ccatggacca gcaccgcttt ctttcagaga
      421 taaaagaagc ttatttgaac tgttaaccta acaagcaaac agaatatgaa acaacataag
      481 atagggatca tcatgaacgg cgtaaccgga aggatgggca ccaatcagca tctgatgcgt
      541 tcgattgccg aaatcatcaa acaggggggg gtgaaaacgg gtgcggatga agtgatcatc
      601 cccgacccca tcctggttgg gagggacgaa aataaactcg gccagctctg caaccgttcg
      661 ggggtggacc gttttaccac cgacctggac ctggctttgt cggatgaaca caacatcatc
      721 tatttcgatg cccagacgac gggccggcgg gcggcagcgg tgcgtaaagc ggtccgtgcc
      781 ggcaaacaca tatactgtga aaagccttgc gcggtgagta cgcaggaagc catggaactt
      841 tacaggttat gtaccgatgc cggcttaaaa aacggcgtag tccaggataa actctggctg
      901 ccgggcctta ctaaactgaa aagactgatt cagaatgatt tttttggaag aatattatcg
      961 gtcaagggag aattcggcta ctgggtgttt gaaggccata ccataccggc acagcgaccc
     1021 tcctggaatt accgcaaaga agacgacgga ggcatcatcg tcgacatgct ctgccactgg
     1081 aggtatgtcc tggacaatct tttcggcaag gtccaatccg tctcctgcct gggcgccacc
     1141 catatccccg agcggatcga tgaaaacggc aaggcttatg cctgcaccgc ggacgatgcc
     1201 gcctatgcca ccttcctcct ggaagggggg attgtggcgc attttaattc ctcctgggct
     1261 acccgcgtgc ggcgggatga cctcctgacc atccaggtcg acggctcaaa aggctccgcc
     1321 gtggcaggcc ttcgggaatg ttatacccaa cactatggca ataccccaaa acccgtatgg
     1381 aaccccgata ttgaacaacc catccgcttt ttcgagggat ggagccgggt accggaccag
     1441 gaaaattatg acaatgcttt taaaatccaa tgggaactct tcctgaagca cgtcgtaaaa
     1501 gatgaacctt tcccctggac ccttctcgaa ggggcaaagg gggtccagtt ggcagagaag
     1561 ggcctggaaa gctgggaaaa aagaaggtgg gtggaggtgg aagatttgga ggtgggtggt
     1621 gggtagtgat tggtggtcag tggtcagtgg tcagtagtca gtagtgaata aaactgtaat
     1681 cacaaaacaa ttttacaatt cgacaattca gcaattcaac aatcacaaat catgtctaaa
     1741 acagcactga tcaccggagg tagccgcggt ataggtttgg ggatcgcagt cgcgctttcc
     1801 aggcagggtt ttaacctggc catcaatggc gtcaggcctg aaccggaagt acaggaagtc
     1861 ctggagcagc tccgggccaa cggtaaagaa gtgatctact gtcagggtga tatcgcctcg
     1921 gaggaagacc gcaagtccat cctcgccagg atcaaagagc atttcgggcg tttacacatc
     1981 ctggtcaata atgccggggt agcgcccagg gaaagaaatg acctactgaa aacaagccgg
     2041 gaaagctatg accacgtgat ggacaccaat ctcaaaggcc cctatttcct gacccaggct
     2101 gtggccaact ggatggtgga acaaaaaagc ctggacccaa cttatgaagc acagattatt
     2161 atagtaggct ccatctccgc aaccgtggct tccgtgaacc ggggagaata ttgcctttca
     2221 aaagccggcc tcgcgatgct ggcccagttg tttgccgtcc ggatgagtga atacaatatc
     2281 ccggtttatg aagtccggcc gggtatcatc aaatccgata tgaccgccgg ggtgaccgaa
     2341 aagtatgata aactgattgc tggcggactg accttgcagc ccaggtgggg tacccccgaa
     2401 gatgtgggca aggcggtggc ggccatcact tccgggcatt tcccctactc caccggccaa
     2461 atatttatgg tggacggtgg actgaccctg ttaagattgt aaattcggtt tttattaaat
     2521 aaatccaact gccatgaaaa aaatcaagcg acgcgaggtc cttaagtcgg tcctccccgg
     2581 agcccttgct ttgactatgc ccaccattat accggcccgt gctttcggag ccaacgaacg
     2641 cgtccgggtg gcggtgatcg gtttcaacag ccgcggcaag gaccatatca gcaatttcat
     2701 gaaactggcc aatgtggagg tggccgggct ctgtgatgtg gacaaaaagg tcctccagtc
     2761 cacagcagcc gattttgaaa aaaaatacgg caaaaaacca aagatcgccc aggacatgag
     2821 gaccctcttt gacgataagg acatcgacgc ggtcagtatc gcgacgccca atcactggca
     2881 tgccctggct acggtttggg cctgccaggc cggcaaagac gtctatgtgg agaaacccgc
     2941 ttcccatgat tttaatgagg gcaggatcat gatccgggcg gccgagaaat atcagcggat
     3001 cgtccagaac ggcattcaac tcaggagttc cgccgccatc caggaagcca tccaacattt
     3061 gcgcaacggg ctcatcggca atgtttacat ggccagggga gtcgtcttta aatggcggcc
     3121 ggatatcggt gacaagggca acagtcccgt ccccgaaggc ctggattgga acttatggca
     3181 ggggcccgcg gcggagcgcc ctttcagtga aaactatgtg cattacaact ggcactggtt
     3241 ttgggattat ggcaacggcg acatcggcaa ccagggagcc catgaaatcg acctgtgcac
     3301 ctggggactg gatgtaggtt tccctgatga ggtttcctgc accgggggca agtttctttg
     3361 gaaggattgt aaggaaaccc ctgaagtcct gtccgccacc tttaagtatc ccaaagcggg
     3421 caaactcatc cagtttgaag tcaggccctg gatgaccaac cgcgaagatg aactggaagt
     3481 aggcaatata ttttatggcg ataaaggcta tatgaccgtc aactggtacg atgactataa
     3541 aaccttctta gggcagaagc gggaacccgg gcccagccgc aaagccgggg gcgaccatta
     3601 tttaaacttt atcgaggccg ttcgaagccg ggataaatcc aaactcaacg gccctattga
     3661 aaccgggcat gtctcggccg gcatttccca cctggccaat atttcatacc gcctggaccg
     3721 gtcgttaaaa tttgatccta agaatgaaaa atttatcgat gatgccgaag ccaatcaaat
     3781 gttgtcccgg aaataccgtg ccccttttga gctcagcgaa aaaatctgag gctcatgcag
     3841 cataaccatt cgacctggag gaatctcctg cgcatgccgg tcctcgttgc ggccctgggt
     3901 tatctcgtgg acatgtacga tctcttttta ttcagtgtag tgcgggtacc cagcctcacc
     3961 gacctggggg taagccagga tcagctgctt cccatcgggg tcagcatcct caactggcag
     4021 cttatcggct tgctcattgg aggcatcctc tggggcatca tgggggataa aaaaggccgg
     4081 ctttctgtat tgttcggatc catcctgatg tattccatag ccaatctggt caacggcctg
     4141 gtacagacgg tgccccaata ccaggccctg cggtttatcg caggcatcgg actggccggg
     4201 gaactgggcg ccggaattac cctggtggcg gaattgctgc ctcagcgctt gaggagttac
     4261 ggggccgtga tcgtttccac catgggagtg ctgggcggca tcctcgcttt cctggtcgcg
     4321 gacctgtttg aatggcgcac ctcctatttt gtcgggggcg gcatggggct ggtcctgctg
     4381 gtcctccgga tccgggtttt tgagtccggg ctgtttatca agcttaaaga aaaaaaagtg
     4441 gcccggggag acctgctgat gattttgacc aatggcaaaa ggttgcttaa atatgtaatg
     4501 ggcatcctgg tgggtatccc aatgtggttt gtagtcggcg tgctgatgac tttttcacct
     4561 gaattcggaa acgcccatgg gctcagcggg atcaatgcgg gcaaggccgt catgctcctc
     4621 ttcgggggcc aggccttcgg aaatatggtg agcgggctgg tgagccagta ttttcaaagc
     4681 cggaaaatcg gcatagggat ctttatgatc ggatctttcc tgaccatgct ggcttatatg
     4741 ttgctcccgg tcaagactga atttcagttt tacggactca tcctggtgct gggcttcttc
     4801 aacggttact ggactttatt tatcattcag gccgccgaat tattcgggac caatatccgc
     4861 gctacggtgg ccaccaccat tcccaatttt gtccggggta ccgcggtcat cctcaccacc
     4921 gcctttgcgg ctttgaggcc cggcatgggg atcatttccg gaggagtcct gttggcttcg
     4981 gcctccgtct tcattgccct gatcgccctc tttttattgc aggagaccta tcacaaggat
     5041 atgaattacg aagagtgatc cggggaacac tcaggcaata cctttagggt acctctaaaa
     5101 acccctcatg gtttaatcgg ttttttatat taaaaaacct aaattgctct tttgaattta
     5161 tactgcgttg aatctcagtc acagagagcc ccgctatgct ccttcgattc gccttgtcta
     5221 aattcaaaat agctaatttt caaactcatt cagggtattt agaggtaccc tttagttttg
     5281 tagcaatatg aataaatccc tctgtttcct gattttcctt tttgcggccc aggcctgtaa
     5341 aaccgtacag gacgataaat ccactcccat gatcagaaaa gacattcaca gttatgccga
     5401 agacgcggtg atcaaacaac tcatactgga cctggaagcc gattttgaaa accgggtcct
     5461 gaagggcagt gggacctatc agattgaaac caatggagcg gattatattg tgctcgacct
     5521 gcggggcccg gaaatattca cggtcctggt cgacggagaa aaggcggatt tcaaattggg
     5581 aaccgaagac cccatcctgg gacaggcatt ggtgattccg gtaaaaccag gctccaaaac
     5641 ggtatccatt ttctatcaaa ccaggcccga tgccgatgcc ctgcagtggc tcagcccgca
     5701 gcagacctcc ggccaccaat atcccttcct gttttcccag ggccaggcta tcctgacccg
     5761 gacctggatc cccattcagg acagccccgg cattcgcttc acttaccagg cgaccatccg
     5821 ggttcccaaa gaactgatgg ccgtgatgag tgcaagaaat gtacaggtca aaaatccgga
     5881 cggcatatac cggttcacca tgttgcagcc ggttcctgcc tatctcatgg ccctcgcagt
     5941 gggtgacctt gctttcaaag ccaccggagc ccgcagcgga gtgtacgccg agccatccgt
     6001 cctggagaaa gccgcctggg agtttgggga cacggaggcc atgatcgccg ccgccgaaca
     6061 actgtacgga ccctatgcct gggagcgata tgatctcctg gtgctccccc ccagtttccc
     6121 cttcggagga atggagaatc cgcggctaac ctttgccaca cctacggtga tcgcagggga
     6181 cagatccctg gtttcactgg tggcccatga acttgcccac agctggagcg gcaacctggt
     6241 gaccaatgca gactggaatg atttctggat caacgagggg ttcaccactt atttcgaaag
     6301 aaggatcatg gaagcgctgt atggaaggga atattcggaa atgctggctt ccctcgcttt
     6361 ggctgaattg cacaggctgg tcctggaaat cgggcctcaa agcaaagaca gccacctgca
     6421 ccttgacctg aagggcagga atcccgatga cggaatgaac agcatcgctt acgaaaaggg
     6481 ttattttttc attcgcctgc ttgaagaaaa aatcggaaga tccaggtttg acgaatttct
     6541 aaaaaagtat ttcagtgaac atgcctttca aaccatgaat tcggaaaggc tgctggaata
     6601 tatgaaacgc catctgcctt tggacagcct ccagatcaat gcggaggaat ggatttacca
     6661 ccccggcatc cctgcaaatt gcccggcccc ggtatctgac cgttttgaca aagtggaaca
     6721 ggcctaccgc cagatggctg ccagccagca ttgggatacc ctcctcacct ccggctggtc
     6781 cagccatgaa tgggtccatt tcattgctca ggtcccggat acggtccatc ctgcccagct
     6841 caaaaaaatg gatcaggcct accgtttttc agaaagcggg aatggagaga tcctggacat
     6901 ctggtttgaa aaggccatcc ggggtggcta ttcccccgaa attctggagc gcatcgagga
     6961 ctttttggtc catatcgggc gcagaaagta tctcatgcct ctttacaagg cttttaaagc
     7021 tacaggccag attgaaaccg cgcgaaggat cttcaaccgg gcgaggccca attaccattc
     7081 ggtttcggcc aacagcgttg agcagttgtt gaaggaataa tttagttagc tagttgacag
     7141 ttagcaagtt agtaagtcag gaattacaac ttgctaactt actaacttac taacttgcca
     7201 actaacctac ttcttcagtt ttacaagcca atccgcaatc ttctctacat cctttttggg
     7261 cacctgcggc atcggcatca tcggtggata gccgggccag cgggactggt cgggagcgta
     7321 gaccgcctgg actaattccg cctttttaag ccctttggag gcgatctcca tccaggatgg
     7381 gccgatgagt ttgacgtcgg ccttatgaca ggtgaaacag gtatgtttga cgagcaattc
     7441 attgacggcc ttgggtatct ccgtctgagc ctggctttca aggatccctc cgaaaatgaa
     7501 ggtcacggcc aggaggatca tcggtttaat ttttttcatt ggaatatcgg acatcttctt
     7561 tcaaatctca tttaaaacgg atggcaaaga taaaaggtta cagggatatc aatagtcaat
     7621 agtcaatagt caatagtcaa tggtcaatgg tcaaatcggt tgccagtaat cggtagtgag
     7681 gaaaggtttt ttactctgtt cacagctcaa agctcaatac tctcccctat ccccggcagc
     7741 aacagatcaa tgccttcctt ttggaaagcg gccaccgcgg cccctttgtc gattttgata
     7801 taaccgaagg tatcgtaatg gaccccgacc accttttcag cgcccaacca ttttgcggcc
     7861 tgaacagcat cttccacacc catagtaaaa ttatcgccga tgggaaggat gacaaaccgg
     7921 aaaggcccgg aggacctgaa ccattgcatg tcgaggcaaa gggcggtgtc gccgctgtaa
     7981 taaaaggttc cctcctctgc ggttataata aaacccatcg ggctgccccc ataagagcca
     8041 tccggcatgg aagaggagtg gaccgcgtgg gtgcatttga cggttccgaa atcaaaggtc
     8101 cacttgcccc cggtattcat ggggtgaaat tgggtcactc cctggtcgcg catccacatg
     8161 gccacctcaa aggccccgac cacggtggca agggtggcct gcgcaatggc caccgcatcg
     8221 gccgtgtgat cactgtgccc gtggctgagc aggatatagt ccgcggttat ctttgaaata
     8281 tcgatatgcc cggcaagttc gttgccggtt ataaaagggt cgaagagcag gattttttgt
     8341 ttgatcttca cgctgaagca ggaatgtccg taataggtca gtttcatcgg taaatgctta
     8401 tctgcctaaa tgtaatgctg tccgtcgaaa agaaatttat atttgtgttt taataatata
     8461 tcggatgacc gacctcgaag cattaaaatt tccggtgggc aagtttcata aaccggattt
     8521 tgtatccccg catcacctca aagcctggat acaggatatt cgcgattttc ccggcctgct
     8581 cgccaaagaa ttggaggggc tctcccccgc tgacctgctc aggacctacc ggccaggggg
     8641 atggaccatc agccaggtgg tccaccatgt ggcggacagc catatgaaca gttttatccg
     8701 gtttaagctt tccctcaccg aggaacaccc aaccatcaaa ccatacctgg aaggattgtg
     8761 ggccgaattg cccgatggca aggatctccc ccccgttatt tccctcaacc tgctgacagc
     8821 ccttcatgac cgctggtgcc acctgctcga ccgcatgacc cccaaggatt ttcacaaagg
     8881 ctatttccat cctgaaaaaa acagggtggt caacctggat gaagccactt gcctttatgc
     8941 ctggcattgc agacaccacc ttgcacatat ccgacttgcc aaacaaaagg cttcctgatg
     9001 gcatggaggt tgctttttct gatttttttc acctggtccg ccgcatgcaa accgtctgtt
     9061 tccgggtctg cgccgccagg taaaaaggat tccaggacat tgaatgttca ggacttcctg
     9121 gaggcctacc gcaaagaacc cgccggccag gccatgctta ttgaccttcg tacacctccc
     9181 gaatttgagg atggttttat ccccggtgca gtgatgatca attacctgga agacgacatc
     9241 gatctgcaat tggggcggct ggataaatcg aaaacctact atatctattg ccagcaggag
     9301 ggacgcagcg tcctttgtat acaaaagatg gagaatatgg ggttcaaaaa agtgtatctc
     9361 atgaaaggcg gctatgacgc tttcctcctt tccggcccca attaaaacgg ctgatttaat
     9421 tgaattgatt ttttcccctt tgaggatact gcttaactta gttgtacata ccggcccatg
     9481 aaatcacttt taacctggat ggctttcctg cttgtgtccc ctgcctttgg ccagggcaac
     9541 caaggcctta taacctttga acaaaaaata aacctgcacc ggaggatccc agcggagaac
     9601 gaagatttga agagcaggat gcccgaattc cagaccacac ttcatgaatt gctttttaaa
     9661 gaaaatgaat ccctgtttcg gcccctggag gaagaagagg atgccccgat gagcggcccg
     9721 ggaaacggag ggatgatcat gcggttccgc cggcctgaaa ccattattta ccgaaaccac
     9781 gatgccgaat atcaacttgc ccagcgcagc ctttttgaga aaaaatattt ggtcgaagac
     9841 accctcagga ttgcaccctg gaaactcgga atggagtcaa aagaaatcct gggatacccc
     9901 tgcctgaaag ccaccctgga aacggaaaca aaacgcgata cccagtggat tcgcacccgc
     9961 gtcaccgcct ggtttaccca ggatatcttc agtcctttcg gcccggatac ttatgggggc
    10021 ttgcctggca tgatccttct cctcgatatc aatgaaggcg aaacggtcta ttcagccacc
    10081 cgtatcgatc cgtcggccga ccttggtgac cttaaagcgc cggtgaaggg ggaaaaaatc
    10141 agcggaaccc aattcaaggc cctgatggag gaacgaatga aggaatttca gcggaacggc
    10201 agggggccgg gccagcgcat gatccggatg aattaaaaat tagtagtcaa tagtcaagag
    10261 tcagtagtca agagtcagta gtcagggtga gggaacagtt aaaagaagat tttctttcct
    10321 tcaatgactt ctttcagtac cctcgcgttt ttgccatcga gtcttccgct gagcagcaat
    10381 cgcatttcac ggcgcaacat cgctccttcg tacaattgtt tttccagttc ggtctcgggg
    10441 atgagttccg gagcctgcac cggcacatgg gtatccgcat cgagggccac aaaggtaaaa
    10501 aaggcgtgcg tggacaattc ggcatccctg cggcgggaat cgctggtgaa aacctgcaca
    10561 taaatttcca tggaagtgtt gaaagcccgg gtaatgcagg cctcaagggt gatgacatgg
    10621 cccaggggaa tcggtttccg gaaggaaaga tgatccacgg acaaggttac gcaataggtc
    10681 tctgcatgcc ggttggcgca gatggcggcc gcgatatcca tccaccgcat gagattaccg
    10741 cccatcgcat tgcccagggc attggtatcg ttgggcatga tcatttcggt catgaccgat
    10801 tttgattccg agactttttt tgacttattc atactgagtt ttaaaatgga tggagccacc
    10861 cggtcatacc aaggcgcatt gaaattcctg ttcgaaatga tagcagagcc gggccttgac
    10921 ctgcgtgaga tccgggggac ggttggtttc gagatggagg gaagtcactc ctttgtccgg
    10981 atcgcggatc ccgcagggca ctatgtaatc aaagggcgtc agatcggcaa ggatattgaa
    11041 ggccaggcca tgcagggtca cccaacggct catgtggacc ccgatagcac atattttttt
    11101 aggcaggccg ccatgcctgg agttaagcca aaccccggtg tattttgggt ttcggaaagc
    11161 ctcaaggcca tactcagcca gtgtaagcat cacgatgttt tcgaggctgc ggacatagcg
    11221 gtggacgtcg gtgtaaaacc attccatgtc gaggatcggg taaaccaccc actgacccgg
    11281 gccatgatag gtaatatccc ctccccggtt gatccggtaa aattcaatgc ccttattccc
    11341 caggagctct tcattgatca aaagattttg ttcttttcca gaccgtccca gggtaaacac
    11401 cggcgggtgc tggcaaaaga tgaggtaaaa cggcccggtc tctttctgtt tcaggggccc
    11461 ggtttttttt tgaatgatgg attgatgcag ggccgtctgc ctctcccaag cttcccggta
    11521 gggcacgagg ccccagtcct cgagaatgac ctgtttttct aagggcacag ccatggtcca
    11581 atgatcttcg tatccataat cgtcgttttt agtatgtcgg cagccgattt aacgcaaaat
    11641 taaccgcctt taagatatcg ttcatacctg cctgaagcct ttggttatat ttttgattca
    11701 tcgatatttt tcccatgaag tggagtctga cactggtttt atttttaata tgcggcctgt
    11761 cccgggcaca gtccaaatcc gatttaaacc agcacaagtc ttttgaggtg atttacacct
    11821 ccctaaaggt ctatcccaac ccggtcgcga gcctggccac tctggaattt aaattggcac
    11881 agccggccca ggtgcgggta agcctgctca atatccttgg tgcggaagtc agggaaatct
    11941 tgcgcgaatc cctcccccag ggcaatcaga atatccggtt cagcgcagac cagttggaaa
    12001 aaggcactta ttttcttcgg ctttcggtca acggggatat tgttaaaacc atccgggtgg
    12061 ccgtcagcaa ccgcacctga tcccttcctc ttgacttttg gcacgatctg tgttagcttt
    12121 agtacttgtg attcaggcaa gtatgaaaat ctgtttgatt atttggttta cagccatctg
    12181 ctttctgccc tgctttggcc agaagaaggc aaaggacttt aaagtcacca ccacggacct
    12241 caaaaccatt gagttgtaca aggactacct caacaagggc aaggtggtga tggtccagat
    12301 cttttttgtg gattgcccgc cctgtaacga gatcgctccc aatatttcag cgctctacaa
    12361 aaaatacggc agcggcagca aacgcgttga attcattgaa ctcagcaaca aatcctggga
    12421 caacaatgcc gcggtccagg gttataagac aagatataac ctcaattttc cttccgtgag
    12481 cagcgaagga ggcagcgttc aggcggcagc cctgtattcg gacaattctt atggctcttt
    12541 cctgggcacg cccactttta taatcattga tcctacgggc ggcgtcacct ttgatccgag
    12601 gggacaacac gcagatgcaa cgacagtcag gctggacact gccctcgcca gggccctgag
    12661 ggtgcccacc gttcccggtt cgggttccgg gggagaggga aacaatccct ctaccgggat
    12721 caaaagggat accctcaacc ttcatggaaa gctaagcctg ggtagttcaa cgctcgggct
    12781 ggtgaccctg aaactcagct ggaacaaaaa atcttatacg ttcagaacgg acatccaggg
    12841 cagttttaag tttcaggcga tcgatacggc aagaacgacc aacgacatcc gcatggaagt
    12901 ggaatacccg ctcgattaca ttacttccgt ctccaccata gacctgatcc tgattcaaaa
    12961 acatatcctc ggagtaaaac cttttggcga ttacaaacaa gtgatcgcgg ccgatgccga
    13021 cctcaacggg gaaatcaatg tcgtcgacct ggtggaactt cgaaaactca tccttggcct
    13081 ccaggacaaa ctcaccgctc catcctccca ggtcacctgg atcaaccacc gcaacctgat
    13141 ccggaatctt tcccaagcca tcccgcttga agatattaag tccctgggca gtaacggcat
    13201 acagctggaa atcgtaaaaa cgggggatgt gagtttgtat aatcagtagt caatagttag
    13261 tagtcagtag tcagttaaca gtttatcagc ctgacaattc agcaattcaa caattctaca
    13321 attcaacaaa aaagggcctc cccgaccagg agaccctcca ctatataagt gtgaaacaaa
    13381 cagttcagat aatagtattc agcagtcagc catcagcagg caggcctatt tcatataatc
    13441 cttggcactg ggattaaatt cagaccagcc attggtccag ttggtggatt ttaaagcgcc
    13501 tatataatcg acattctcaa aaaaggcggg cagacccgta ttgcttcctc ccttctccag
    13561 tccgtcgctc aggccggtgg tgagcaccaa agtaggcttg gcggtgaaaa tatccgcctg
    13621 gaggccggtc tggctccttt tcaacaggat cttattccct tcaatccccg caaaccattc
    13681 cgatggtttt ttatcgccga tcttgatatc cgcaggagga ttggcggtat tcaggtcacg
    13741 gatggcataa ccacgggggt tatggttggt cccgtttcca aatccgttat caccccatcc
    13801 atccagtccg gcaaccacca cattcttcaa ttggagttcc ccgttggcag cgttggcctg
    13861 ggtggtggaa ccatcaatgt atatcccatt cgggtacccg gtgacgaccg tgttatatat
    13921 tttgattttg gtatttctgc gcaggtgcat tccgttctgg aattgagggc tgatggaagt
    13981 cgccacctcg gctttggggc caataatgct gacgttggaa aaatagggcg cggtgaaagg
    14041 catcgcggcg ctgccgcttc catcattgtc cacttcaaat ccattggagc cggattgatc
    14101 ggcctgggtg gctcctctca ggccaatggc aaattgtaca ttcccggaat acccgttgtc
    14161 gacgtcgaaa tcatcatcca ggccccgata ggcaaccagg tatttggcgt tcaccgtgcc
    14221 gccaaaccat tcgaaggcgt catccaaccc ataggagcat tgcacgtatt ctattttggt
    14281 gccccggccg accgatccca tcgtcagtga attcacctcc tggttgggat tgatgggaat
    14341 gccggcatat tcaatccgga catatttcag gacaccggaa ttgtcctcat cgtcattgcc
    14401 gccatggaaa gctccatatt ggccttccag ttcaccggta ccccctggca aattgttctt
    14461 ggctttcccg caaaggacca gtcctcccca gtctccgggt tcacgttcgc cgatcgcccg
    14521 ttctgaagtg aagatgattg gttcggctgc agtgccttca gcaataatcc tgctcccgcg
    14581 gtgcacgatc atcgtgcctt tggtggcccg ttcaccaatg atcacggtac cggcctggat
    14641 ggtgagtacg ccggttttag tgggatttcc gtctttggtc agatcttccc ctacccggac
    14701 aaaacctttg agccggtaaa ccttgtcctt ggtccagctg acgttacctt ccaggatccc
    14761 gctgacatcc acgatgggtt ttacaggaag tgccgatacg gtaatgacct ggggcccgcc
    14821 gactgtgacc tggtcgtcgt agtccgacac cacgaaggtg aagttgatgt tcgtacccga
    14881 aggcagggct tcgatcgtat aggttttgct gtactgatcg gttttggtcc cttcggaata
    14941 ggttttatag tcaaagtctg ctccgttttt cagcacctga agggttttta agcccctttc
    15001 agattgcagg ctcacggtta tgtttacaac ggttccggct aatccggaag cagcggttcc
    15061 gggggtcaca acgggtgccg gaaatacttc atcctccttt ttacaggagg tctggatcaa
    15121 catccaggcc cccagcagca agagggatga aagggtaagc agtggtttag tttggatctt
    15181 cataataatt acatttatac cttttggttt aataaatctg ataagtcagg ccggcctgta
    15241 ccagacggcc gggagcatat tgttgaataa tctggtccct ggacggatcc cattttttgt
    15301 cctggttgcc atcctggagg atgcgcgccg gttgattcaa gaggtccgat acgccggctt
    15361 ttaatattat cctggagccc agttccttgg aaatcatcaa gtccagcaca ttccggggca
    15421 tttcgtagag gtcgggataa ccctggaatc caacggcaaa aatcctcttg ccgatcacgt
    15481 tgtagaggac atttacctgc agtccttgat cggcgtcaag gtagttcaag gcggcattca
    15541 cgataaaggg agactggccc tgaagaggac gctggtcgga ctggccttcg ctgatgccat
    15601 cacccaggcg gaccgctgaa tggatcaggg ttgcgttaaa cacggtgctg aatcgcccca
    15661 ggaaccggga tggcgttagg ttcgataagg acttctttaa ttccacctcg acgccataat
    15721 tcacgctgct ttcggcattg gcaaaggtga aggtctttgc cccccccgag cccgaacccg
    15781 ggaccaccag ggattcgatc gggtctttga acttcttgta aaacagggcc acattgaaaa
    15841 cctcaaaggg agtcggataa tattcaaaac gggtgtcgaa attctggacc accgcggttt
    15901 ttaacgggga acccttgtac acgtaattgt agtcaaaatc ataataggca aaaggcgcca
    15961 gttccctgaa ttccgggcgg ttaaggctca tcccatacga tgctttcaga atggattttt
    16021 ggctgaaata ataggacatg gtcgcggagg gaagccattt ggttattgga taatggagtg
    16081 ccaccggggt ccccccgatc agggcgctct ccaggttttg ggtattgtgc tcaaggcgga
    16141 gcccaccgga aaaggaaagc tttttgccaa gtggagtcag gacatggaca aaaccggcat
    16201 ataatttatt tgaggaacgg taggaatcgc tgggattggt ctgctcgtcg attttgacgc
    16261 cggtccggtt attgatattg ttgaacagtt cggtgatatc cccattgatc agatcattgt
    16321 tgaagtcaaa agcgcttccc cggacatatc ccaggttccg ggcgtcgaaa gtccggtttt
    16381 tatcctccag ggcgcccccg gctttgattt ccagatcgtt tatttttttc gaaacggcca
    16441 ggtttccgga ataagttttt tcaagcatct ccgaggaaaa ccggccgagg aaaaaggcct
    16501 gagccgcgcc aaacggaacg tataagcttg aggtgcccga tgattcgtca aaatcggacc
    16561 ggtatctccg gtaatcgggt tgattccggg tggcccggtt atacccaaag gtccattcca
    16621 agttcaggga ttcgtcctgc aggaattgat gggttcccac cagttgactg gaaaggatgt
    16681 tcttatagac ctggtcaaag gaatggttat tgggaatgta gttggattca aagtttttcc
    16741 ctgaacggta aacataactt ccggtgctgt tctgattgaa aaggtttttc cattcgatct
    16801 gatggttgtc ccattttgcg gcccagttgt gaagtacccc gatccggata ttccggttgt
    16861 actgggcatc atcataggag aagatccggg attcaatgcc attggcctgt tcattgtaat
    16921 ccccgcggtt tatcccgaat tgggttttgg aatcctggta ggtaacggaa gtgatgtttc
    16981 cgacctggac cccgccgaac accgtcctca ggttattggt caggttgatt ttttggtcag
    17041 gcaccgcgac agtcggggtg gccacccagt tgtttttgag ggactttccg gcgagttcca
    17101 gctggatggg gttattgatg acctcattga ccgaggcggg gaaatttttc ggaaggtcaa
    17161 aagtcccgga attgaaaccg gtccaatagt tttttccaag ctggggttga taaaaatcgt
    17221 tgaaagtggt tccctgcctg aagccggccg catattcgac actgaggccg gatttgctgg
    17281 gaattccttt ggtaaaaatt ttgaccaaac ctccggcaaa ttcacccgga agatcggcgg
    17341 aagggctttt aaacaccagc atgcgctcga tctgcccggc cgggatgata tcaaacgaaa
    17401 acgatttgat atccgtttcc atggaagggg tgaaggcatt gtgcagcatc acgttgttgt
    17461 accttgaatt cagaccgcgg atattgataa accggtcccc gacaatggtg atgcccggca
    17521 ccctccgcac aatttgggct gcattaccgt ccatggattt tttgatctgc tgttcggaga
    17581 ttccactgac tacctgcatg gaggctttaa tctcggagag gatggccact tcactgttcc
    17641 gcactttatc cccggtgacc accacttcct gcaaggtttc ggtttcctcc accaggtcca
    17701 gattaatctg tgtgatcctg ccggcttcca cctttacccc cgcaagattt tccgtacgga
    17761 atgaaacgta ggatatctga agggtgtact ctcctgcagg tacatggtct atcgaaaaga
    17821 atccttcgat atccgtcgcc gatcccaatt cggtcccgat caccttgacc gtggctccaa
    17881 tcaggggctc ggataatttc tggtcggtaa taattccctg gatggtcccg ttttgtgcat
    17941 tcaaccggat aaaacccgaa acgagaatca acagggtaaa aaacgtttgt ttcatgtcta
    18001 ctctttgttt gtgcagcaaa agtagacggg ctgtattaag ccaatgttaa taccggatta
    18061 aggctatatt acgttttatc atattgcaat tcagcatttt atgtaataaa tgcaataata
    18121 tatgtggatt tagtctggaa ggtgaggagt ttgaatactc aaacagagat ctgatcgctg
    18181 atgtctgata actgatcact gacaaccgaa atctgattgc ttatcgctga tcgctgattg
    18241 ctgatcgctg gtcgctgata gctgtctgtt aatttttctt tgatcttaaa gccctttggt
    18301 tttccgcatt cccggaagga aagacctcct cgacgtattt ccaaatcccc ttatccagcc
    18361 ggaaaccttc gtaagatcct tcggggaccc tggtcctgcc ctgccctggc aagtttcccg
    18421 gctgttccat aagatggtcg caaatgatca ttttttcatt ttcatcgtaa ttgacccgga
    18481 cggcggcgtc ggcgctgtac tgatagatga tgcggtggcg gatttcgttt ttacccatcc
    18541 ggaacaccgg ggccccgaag acgggtttgc cttccctgaa atgaaggaca tcgatgactt
    18601 ttctacgctc atagaccgaa tggccgtcat aaccaaaaag cagccaggcc ctggatttat
    18661 cggccatttc gaagggaacc atattgtaat agaccagtcc ataccatctg tccgcgggca
    18721 gcgacaccga ttccggatca gcaagttcat gggagcggtc ggcaaaggaa gtgagtttga
    18781 gggagggact gttccactgg agagcgccga aataacggta atctgcctcg ttcactttca
    18841 attgccagct gaaaatgcga aaactgctgt cactcggata agtgatggct acattttcca
    18901 atcggggaaa tttgtattgg aaggaatggt cgaccttgag cgccttcgcc agggtgggta
    18961 tcatctggtg gcaggcatag atccgctcgt cctcgatttc ggcattcagg actcccctcg
    19021 cccagacggc aatggtgtct tcataattct ggatccggag taaatcggtt cctgaaaggt
    19081 ttcccgactg ggcatggccg tttaacccaa tgaatgagca taaagctaca aacaagatcg
    19141 agcaggtccc tttcagccgc atgctgaaga taacgctgat taaagaaggg ggtttgctaa
    19201 ccaaaagtta aaaatcagag cccgagggcg tcataagcgt ccatcacgga cttcacggct
    19261 tgggcggaag cattgagcaa ggccatttca tcttcgttca atttgagttc gatgacctgt
    19321 ttcacgccgc ccgcgcccag ttttaccggt actcccacat acacgtcccg gaggccgtgc
    19381 tggccctcca ttttaataca acaggggaaa atccggtttt catccttgag gatcgcggtg
    19441 gccatttgcg cggcggcggc acccggagca taccatgcgg aggtgcccat gagttttacc
    19501 agttctccac ccccgacttt tgtcctttcc atgattttat taagggtctc ggtgtcgatc
    19561 agctcggtga ccggtattcc gctgacggtc gtatacctgg gaagcggaac catggtatcc
    19621 ccgtgccccc cgagcaatac ggcctggatg tctttcgggg acacctggag cgcatccgcc
    19681 aggaaagccc tgtacctggc cgtgtccaga atgccggcca tgccaatcac tttggcagtg
    19741 ggcaggcctg aggttttctg ggctgcataa gtcatgacat cgagggggtt tgaaaccacg
    19801 atcaggatgg ggtctttgga atgttcaagg atggatttgg tcaccccgct tacgatcttg
    19861 gcattgatgg tgatcaggtc atccctgctc atgcccggtt tccggggcac gccggcggta
    19921 aggatcacga tgtctgaacc tgcggtatcg gcatagtcgg aggtcccgat aacccgagtg
    19981 ctgtaatcat cgatcggggc ctgctgccag gtatccaggg ccttcccctt ggccaggtct
    20041 ccggcgatat ccagcatgac gatttcattg acgatatcct ggtgggcgag gacattggca
    20101 caggtagcac ccacatttcc ggcgccgata acggtcactt tactcatatt atacttttat
    20161 ttaatattta tttatggaga tcaaaggtaa gcggatgcgg ccaatcggca aaagatatcc
    20221 ctcatctttg tacaccgggg gatgaatatt ttctgttaat tttgtaggct atatgagatg
    20281 gtttctgatc gcaggaagtt tgttgttttt tagtgcagtg aaagcccagg aggacaaacc
    20341 ttttttatgc ggtacggaaa atctcgagga aatcaagcag gtgatgctta aaaaccggga
    20401 agaattcaaa aacttccagt ttcagcgtgg ggccatcagc tatattccgg tcaaaatatt
    20461 cctggtcgcc tacaacgacg gttccggcca gatgcccgtc ctcaatgccc tgcgcatgct
    20521 ttgccagttg aataccgcct atgcggacat ggaattgcaa ttttacatca aggagatcaa
    20581 gacgctcaac aacagcacca ttaccgaaac gccaaggtct tccgggggaa ccctgcaaat
    20641 gcagctcaac aaagatccca aggccgtcaa tatattcatt acgcataaaa tcctcgatgg
    20701 cgtggcgggt tattatatcg gaccggccgg atccaataac gactttatcg tgatccagaa
    20761 agactacatg ggagatgtcc gggtggcggc acacgaagta ggccattacc tgagcctccc
    20821 gcataccttt catggctggg attccgaagc ctgggacccg gtcaaacacg gcaatcccgt
    20881 ccagaaattt gcacccgacg gcatcaccat caatgaatat gcggacagct ccaattgtgg
    20941 tccgaaaaat gtgggtgatg gattttgtga taccccggca gactataatt tcggggccaa
    21001 tacctgttct tattctttaa aagccatgga ccccaacggg aagctggtct cgccccagac
    21061 caacaacatg atgaattatt ttttcggctg cagcaaatac ctcttcaccc cgcaacaaaa
    21121 agcagcgatc ctgtccagct acaacagcac ctcgcgccgc gatatccggg ggacttcgcc
    21181 ctccgaggtc actccaatca accaggcggt aaaattgctc aagccggccg ataaatcagc
    21241 cacggccagc gcggacagcg tcctgctgga atgggaagcg gtggccggag ctgcttcata
    21301 cctggttcaa tacgatatcg taccgacctt cgaggttttt gtccagacgt ttatcaccaa
    21361 agagaccaaa caaaaaatta ccggcctcgt actcaaccgc acctattatt ggagggttat
    21421 cccttttaat gactattcga cctgttaccc tgaccctccc cgcttttctt ttaaggcggg
    21481 cgccctcacc agcgtgcggg atatcccgga ggtcaagagt ttatccattt atcccaatcc
    21541 agtcgccaat caacaactgc tcaacgtcta tttggacaac cacaccggat tttccggctc
    21601 catatccata ttcgatctga atggccgcct ggtcttgcag gtaccggatc agaaattcac
    21661 ggcaggcgct tccagcaaag ggctggatat ttcaggtctt cgcccggggg tctatgcttt
    21721 tgtattaagc agtaggggcg ggctggcgag ccggcggttg accgtttttt aagcgaccgg
    21781 gttcaaccag cctgtttttt ccgcaaggac cattcaaacg agaaaattgc gaccacctcc
    21841 ccctcccggt tcctcccggt ggagttcata ataattttta ccccggcatc ctgttccagg
    21901 gcaatcgctg cggttttccg catatcctgg acctggttgc attcaaataa aacagggcct
    21961 tttgcctttt tcaaaaactg cccctccacc cgcgtcacca gcatggatat ccgcgacgga
    22021 tccagggagg tcagtgcgat aatgccggtg gacaattccg ccgcggcggc cagtgccgca
    22081 aaatagatgg atttaaaagg gtttttggtg gaccacctgt acgggatatt cacctgtgcc
    22141 cgctcttccg taagagagac caccttcagt ccaaagaaaa aggcgcccgg aagatggagt
    22201 aaaaaatacc agaacatcat ccatttgcta tggaccattt ttgtggtttg tacctgcatg
    22261 gaatgtgatt aatccttcaa taataagtaa tttcgcggca tcattatctg aacttatgaa
    22321 tctgcacgaa tatcagggaa aggaattact taaaaagttt ggcataccgg tccaggaggg
    22381 atttcttgcc accacaccag aagaagccat aaaagcttat gaccaggtgg tagaatcttc
    22441 aaagtcgccg gtagcggtca tcaaagccca gattcatgcc ggtggccggg gcaaaggcgg
    22501 tggcgtcaaa ctggtcaaat cccgggagca ggccgcccaa acggcacccc agatccttgg
    22561 tatgatgctc aaaactcccc agacgccggg agggatgaac ggccctggaa aactggtccg
    22621 taaaatcctg gtgacggaag attcattcaa accggatttt gaatcctgca aggaatatta
    22681 cgtctccatc ctcacggacc gggaaaaaaa agccaatgtg atcatctatt ccacggaagg
    22741 gggtatggat attgaagccg tggctgaaaa aacaccccac ctggtccaca aggaatatgt
    22801 gcatcccctg gccggattcc aggctttcca ggcccggaac atcgccttca agcttggcct
    22861 gagcggggag agttttaaaa acatggtccg tttcgtgacc aatctctaca aggcctttac
    22921 cgagtccgat gccactttgt tcgaaatcaa tccctgcctg aaagacggat acggcaatat
    22981 cctcgcggtg gattgcaagg tttccctgga tgacaacgcc ctgttccgcc acagcgacct
    23041 ggaagccatg cgcgatacgg atgaagaaga tcccgcagaa gtcgaagcaa aagcctataa
    23101 cctcaactac gtgagcctcg atggcaacgt gggatgcatg gtcaatggcg ccggcctggc
    23161 catggccacc atggacatca tcaaactctc cggcggccaa cctgccaatt tcctggatgt
    23221 ggggggcacc gccgacgccg cccgcgtgga acaggcgttc cggattatcc tgaaagaccc
    23281 ggctgtgaaa gccatactca tcaatatctt cgggggcatc gtgcgctgtg accgggtggc
    23341 ccaaggggtg gtagacgctt acaaaaacat tggagagatc aaggtgccga tcatcgtccg
    23401 gctccagggc accaacgcag agctggccaa ggatatcatc gaccagtctg gcctgcgggt
    23461 gaggtccgcc atcctgctga aagacgcggc agaactggtc agggaagcat tgttggaaaa
    23521 ataatttata cccaccggct tgaccaggat ctctttattc gaattaaatg aatacgtcag
    23581 gcaggtcatc gcccttaatt ttaccgaacc actctggatc cgggcagaaa tcaaccaggt
    23641 aagtttttcc aggggccatt gttatctcga actcatcgaa aaaaaggggg atacggatga
    23701 aatcatcgcc caggccagcg cggcgatctg gtatcgaaac ctgcgcttta tagaacaaaa
    23761 aacaggtcct aatcttaacc agctcttaca gaagggaata caaacattgc ttaaagtaag
    23821 agtcgaattc aatgaacgtt atggactgaa attaagtgtt gaagacatag acaccacctt
    23881 tacgattggg cagcttgaat tgaaaagaag ggaaattctt caacaattgg gagccctggg
    23941 actgatggat aaaaacaaag gccatgccct gccgccggta cttcaaaggc tggcggtcat
    24001 ttcatcggaa aaggctgcgg gctttcaaga ttttatccag cacctccagg ccaatgacta
    24061 cggttttgcc ttcgaatgtg aactctattc cgcggccatg cagggaagga atgtggaaaa
    24121 ggagatcctg gctgctttcc aggaaatcag gcaggcgcga cagccttatg acgcggtcat
    24181 catcatccgg ggcggcggat cccggctgga cctggccgct ttcgacagcc tgcagctctg
    24241 tgtggctatc gcaaatctcg aatacccggt gattacaggc attggccatg aaaccgatca
    24301 ggccgtggca gacctggtgg cgcataccag cctcaagacc cccactgcgg tcgctcattt
    24361 tgtcctggag cacaacttca aattcgaaac ccagctgtat gccggatacc ggatccttca
    24421 aaaatccgcc ctggatatca tcgaatcgtt tcagtccagg ctgaggcggc aaagccagat
    24481 gattaaggat atcagcctgc gccgggtggg caatgaaaac tactttatgg cagcggccaa
    24541 aaaggattta aaaatgtata ccctgcaact cctccgggaa aagcaggccg aactccacaa
    24601 cctgcagcag agaacagcat tgatggatcc tgaactcctg ctgaagcggg gatacagtat
    24661 gaccttcaag gacggagtgc tgttgaaaag tccggaccag gcggaaagcg gggacctcat
    24721 cgaaaccagg ctttccggag gcagcatccg gagcacggtt aaataataag ttttacattt
    24781 accgggtatg tcgaaaacca ggaaggacaa agggagtttt gaagaagcca aaagagcgct
    24841 ggacgagatc attgcagaac ttcagaacga gaacacctcc atcgacaaac tggcctccca
    24901 ggtaaaaagg gcccaggaac tgatccaata ttgccagtcc aggctgagga ccattgaaaa
    24961 ggaggttcac tccctcctgg atgaagaaga atagaccacc cggatcaagg cagccaggtt
    25021 ggcaccaaaa gcctccagat tttctttgcc cattttaata gccgtttcca gatcttctgg
    25081 accccttaca attgaaaatg cggcactaaa acccattttg cgattttgcg ccgtgttcat
    25141 ttgaagactt ccgcaaagcc cgatcaccgg ttttccgcat tttcccgcgg ttttcagtac
    25201 cccatgcacc agtttaccga accgggactg gaaatccagc gctccttccc cggtaatcac
    25261 caggtcatgc gcctgcactt tttcccggaa ccggagcagt tcaaatatcc gtgaagttcc
    25321 atggtgtatg gtgcagccca tgatcccgtg catgccgccc ccgatcccgc ctgccgcccc
    25381 actccctttt accttttgaa ggtcagtacc gaactgattg tgtattaccc gggcaaaatg
    25441 aatcatcccc ttttccagct ttttccgaat gggaggtccg gcaccttttt gcggcccgta
    25501 aacctcaacc gccccttccg ggccataaaa aggattggtc acatcggtcc agatctcaat
    25561 ggtgcggctg ccttccatct ccatccgggt atccacggca tggatctgga tgaggttggc
    25621 aggtaccggg tccaacaccc tccccttttt atccaggaac cggatgccga ggcggtgcat
    25681 gatccccagg cctgcgtcat tggttgcgct ccccccaacc atgagtatga ccctcctggc
    25741 ttttcgtttt aaggcatgct gaaccagttc gccggttccc atcgtgttgg agatccagat
    25801 gtttttttcc cgtccgttga gcacggccag gcctgaagcg gccgccattt cgatgtaggc
    25861 tgtagaggat tcctgttcaa acaaataggg tgctttaagt tcatggccca gggcatccga
    25921 tgcggtgatc caaaccggct tcagatgcag atggtgggcc agtgccagga ccgagccgtc
    25981 ccctccgtca cacaggggga aggtggtcag ggaatcgggg ttcagagtac cggcaaggcc
    26041 tttccggagg gcgttatttg cttcaaggga agagcaggaa cctttgaaac tgttcagggc
    26101 gagcaggatt ttcatttcat caggtctgcg gggtatttca aaccgatgag tgtccggata
    26161 cggtccaggg tatccatgat ttgcagcgaa aaatgcagcg gcatgaggtc gctttccaat
    26221 tttccggctt gcacacaatc ctggacttct tttatctgat agtagaaacc acggtttggg
    26281 tagtcccagt ttttaatttc cgcttcgccg ttttcgggtt tgatgatcat ttctcctctt
    26341 tcatgccatc gcctgggcaa aagcaccgat cctttttcat aaaatagctg agccgaagtg
    26401 tccgactccc agagaatgct ggattccagg taggcggtga tgccttccgc gtccttgagg
    26461 atgacctggc aggtctcatc gatctcattc aatatggtag cttccgcctg gatcaccgca
    26521 ggcaccccta ataaaagctg cgccagaaag accggataca ctccgatatc aagcagggat
    26581 ccgcctccaa gttcatttac caccagacgg ctgacccctt gcgccgaggc tctttccttc
    26641 cctttaaagg caaaatcggc cctgaggtgg aggagcctcc ccaacctgcc ggaatcgtgc
    26701 aatgacttca ggtaaaggat atggggaaga aagcgggtcc acatcgcctc catcagaaaa
    26761 acctgatgga cccttgcctg atggatcagc ccggtcaatt catgtttgtt gatggtaaag
    26821 ggtttttcgc ataatacggg cagaccggcc tccaggcatt ttgaagtgca ataagcgtgc
    26881 aaatgatgcg gggtcgcaac gtatacggca tccaggcctc cgtgaccaac cagttcctca
    26941 taactgccaa aggctctcgc cgccccgtat tctttggcga aagcctccgc cttagtgata
    27001 tctctggagc ccaccgcggc cagttccccc accccggttt ctttcaattc tttggcgaat
    27061 ttgttggcga tcctgcccag tccaattata ccccacttaa acatgtatta aaatttacaa
    27121 tttgttcaca aaattaaatc cttcaaggac attcgcggtg ctatctttgt aagacgtttt
    27181 acaagaaccc cttgtacccg attccggcaa gatttttacc catattaata atatttactt
    27241 aaacctattt aaacaaatga tctatggcta ttaatctatt ggagttgttg caaagccagg
    27301 tggggggaga attggtcggc caggccagca aattcctggg cgaaaccgaa gtcaacaccg
    27361 ggaaagcggt gggttcgatt ttcccaactc ttcttggcag ccttgtaggc aaagcatcca
    27421 cggaatccgg ttcaaggggg atccttgatt ttattaaaac taacaatata gacggcagcg
    27481 tactgaattc actgggaggt ttgttttcag gaggccaggc caccgattcc ctcctgggtt
    27541 ccgggggagg gatcctcaag tttttagtgg gcgataaact cggttcgatc gtggacgtca
    27601 tatcgaatgt ttccggggta aaaacaggga cttcatcctc cctgctcaaa atggcagctc
    27661 cgctcctcat ggggtttgtg ggcaaatttg taaaagaaaa agccctggat gccatgggtt
    27721 tgaaaaacct gctgggcggc cagcgtgaat tcatttccaa ggggatcccc ggagccctga
    27781 ccggtctgct gggcctggca accaatgccg gtggcggagc tccaaaaatg cccgcaggcc
    27841 catcctcaac aaccggaagc agtaacttgc tcaaatgggc cttgcccatc ctgctgttgc
    27901 tcgcggtcat ttatttcttc ggacgcaaag gctgcgaccg tcaaatggag accacgccca
    27961 ccgaaatggt tgacagcgcc aaactcgccg ccgaggccgc cgcagccgaa caaaaacgga
    28021 ttgcggacag cattgccgct tccagatctg tttatacgct gcccggaggc atatccctga
    28081 ataccaaaaa aggtagtttc acggacaaat tcgtatcctt ctttgcggac agccttgcaa
    28141 aggtcgatcc caaaatggcc tttactttcg acggggtcca ttttaaaacc ggttccgatt
    28201 ccctcaccat ggagtccggt acccaattgg atgaactggc cagtgtaatg acggcttatt
    28261 ccggggtaaa catccgggtg gtcgggcata ccgataatgt gggggatgcg gctatgaata
    28321 aaaaattgtc cgacgcccgg gccaaatccg tcaaggccta tctggttggc aaaggcattg
    28381 ccggcaggcg cattgaaacc ctcgggatgg gacaagccag cccaatcggg gacaacaata
    28441 cggaagaagg gaaagcgatg aaccgccgcg tggaggtttt cgtcacccgc aaataaatgt
    28501 tggatttatt caatgaaagc cagtctcccc ccggactggc ttttttgttt caacggacaa
    28561 gccctggcag ggttacttgt ccaggatccc gatcacaaga aatcccggcc ggacgttcag
    28621 ataataccat aaatttgaac gtatatgcta ctgcgcgtga aattgccagg aatgctggca
    28681 attgtttttt tggtgttttt aaactccagc attgaggctc aatctgtttt aaaccgcttc
    28741 caggtcatcg ggaggccaaa acaaatcgac atccccttcc agtttgagaa taattttatc
    28801 ctggtcaagt ctttgttcaa ccgctccttc cccctcaatt ttattttcga tacaggagct
    28861 gagcatgccc tgctttttaa acgggagtat gcggatatcc tcggactcaa atacgaccgc
    28921 aggatccggc tgatcggggc ggatcagtcg gaagaaatgt atgccctgat tgcccggggg
    28981 gtttccttcg gtctgagtcc gacggtttcc tattcccagg acatcctggt cctggaacag
    29041 gattttttcc atctggatga ggtgacggga ttgtatgtgg acggcatcat gagctcttcc
    29101 ttttttaagg ggtatataca gcacatcgat taccgtaagc ggatcctgtc ctttattcaa
    29161 cccagcgttt tccgctataa ttcacggaaa tacaccaagg ttcccgtgga gatcatcaac
    29221 ggaaaactct atgtaaccgg ccaggtcgaa atacaaaaca aaaagatacc tgttaaattg
    29281 ctggtggact ccggcgccgg cctccccctg ttgctataca ccaatgccaa tcccgatttt
    29341 gccccccctg agaaagccat acccggacgc ctgggcaagg gcctgggtgg ttaccttgag
    29401 ggtcatatcg gcaagatcac ggaactccaa ttagaaaagt ttaaattccc caacatcgta
    29461 accagttttc agcaactgga ttcgaccctg atccccgcgg gagccgagga aacccggttt
    29521 actcgcgacg gattgctggg gaatcagctg ctcagccgat ttgacattta tatcgattac
    29581 aacaaaaacc tcatgtacct gagcccgatc aaagacctca gcaaagaatt tctggtcgac
    29641 cgcagcggaa tggaaatcct ggctaccggg catgaactga aacaatacat cgtgaattcg
    29701 gtgatccccg gaagcccggc cgacgaagca ggcctcaaaa aaggggacat catcaaacgt
    29761 atcggattcc tttccaccaa catactttcc ctggaacaga tcatccattc cctggctcgg
    29821 aaacccggca aaaaagtcag gatccagtat ttcagaaatg ccaccgaata caaaaccacg
    29881 gtaaccctcc ggtccttgat ttgatgccat gcgtggataa aatatcggcc tccctttttt
    29941 ctccacatcc cgcatgatac ctttgtttgg tgtactccag tttaaaagcc tgtgtggaag
    30001 accttgaacg acaagggcaa ttgttcaggg tgaaagagga agtggacccc gacctggaaa
    30061 tggcggagat ccatcgccgt gtatttgatt ccgggggccc cgccctttta ttcgaaaaaa
    30121 taaaaggttg ccggtttccc gccgtttcaa atctttatgg caccagggac cgggtcctct
    30181 atttatttag aaacagcctg gaagacctca aggcgttggg caggcttaaa aacgacccgg
    30241 gactgatcct aaaaaaacca ttttcctacc tgccggccgt tttgaaagcc cgctttgcct
    30301 tgcccaggaa aatcaggttc agccggagta tggtgaatac ctgtagcatc tcagacttgc
    30361 ctcagatccg atgctggccc atggacggag ggccttttgt cctgcttccc caggtactga
    30421 gcctgccgcc gggctcccgc gacctgctcc agtccaacct cgggatgtac cggatccagc
    30481 ttggtggaaa cgcctatctc cccgaccagg aaatcggttt gcattatcag ctgcaccggg
    30541 gcatagggat ccatcataaa atgtacctgg agagcgacga accattccgg gtaagcgtgt
    30601 ttataggcgg cccgccttcc cacagcctct ccgctatctt tcctttgccc gaggacatgt
    30661 cagaactcac tttcgccggc ttgctcaacc gtaaacggtt ttcctggttc ctgaaagacg
    30721 gttatgtgct ttcagaccag gcggactttg tcattacggg caccatcgac aaaagcctga
    30781 aaaaaccgga aggcccgttt ggggaccacc tgggatatta ttccctgacc catgattttc
    30841 cggtgatgaa ggtggacaaa gtttaccacc gtaaggatgc cctctggcat tttacggtcg
    30901 ttggacgccc tccccaggaa gattcctttt ttggattcct catccatgaa attgttaaag
    30961 aactgattcc ccgagagttt ccgggcctca ccgggcttca cgcagtggat gtagccgggg
    31021 tccatcccct cctgctggcc accggaaaag agcgctacat gcccttccgt gaacccaggc
    31081 cggaagaaat cctgaccatc gggtcacgca tcctcggctc ggggcaaacc tcactggcta
    31141 aatatctgtt catcgcggcg ccggtggaac gggaaaagct tgacctctat gacctgcccg
    31201 gtttttttga ttatattctt cgccggattc gctttgatcg cgaccttcat tttatcacca
    31261 acaccacgat cgacaccctc gattattcag gagaaggctg gaatgcggga tctaaggtca
    31321 tggtcgcctg ttgcggcccg gtgatccggg acctgtcgga acgcttgccg gagcacctgg
    31381 ttttcccacc cgggtttatg cgtccggtct tgatccaggc cggtattgtc tgtgttgaag
    31441 gtacccgctt cacggaccat tcaaaggaaa gccaggccct ggatcaatgg accgccagcc
    31501 aggaacccga caattggagg ggaatcgccc tgatcgtact ttgtgatgac tccgctttta
    31561 ctgcgtccaa ttgggataat ttcacgtggg tgacctttac ccggtccaat ccctcccacg
    31621 acatgtacgg aatcggctca ttcacttcgt ttaagcattg gggttgccgg ggaccgctca
    31681 ttattgacgc ccggatcaaa ccccaccatg ccccggtcct cgaagccgat ccggctacca
    31741 ccgcaaaggt ggatcgtaaa ttcagtaaag gaggatctct gtatgcgctc caccggaagt
    31801 aaatttggat gaagcccagc accccttatc ccctgcccga tgcatttctc aaaaggatgc
    31861 aagatcagct gggtacctca tattcttctt tcctggatgc cgttgaaaaa atctcacccc
    31921 cggcggtccg gctccatcct ctgaagttgg ccggccatta tgagtttccc ctggatctaa
    31981 atattccctg ggaagatcac gggtattatc tccgggagag gatcaaattc actttggatc
    32041 cctcgtttca tggaggagcc tattatgtcc aggagcccag ttccatgttg gtcggcccat
    32101 tggtaaaaag actcctggag agctacgatc agcctctggt cctcgacctt tgcgcatcgc
    32161 ccggtggtaa aagcacggcg atcctcagcg ccttgaacgg gcgcggcttg ctcctggcga
    32221 acgaaactat tgccggcagg atcagcggcc ttcagcacaa cctcatcaag tggggatatg
    32281 ccaatgccct gatcagccaa tccgatccca ttcaatttca ggcttttccc ggcatgttcg
    32341 atctcattct cgtggatgcg ccctgttcag gagaaggttt gttcagaaaa gaccattcca
    32401 gccgaaagga atggacaccg gacctggccc tccaatgcag ccttaggcag aaaaggatcc
    32461 taagggatat tcttcccgca cttaaaccgg gcggaatact cctctattcc acctgcacgt
    32521 ttaatccggc tgaaaacatg gaacaactcc acatgcttgg aggcctggga tttgaatcca
    32581 tccctctgaa ggaatgggat gatttcccat tagtccgcct ggaagaaaac ggggtcaccg
    32641 gttaccaggc ctacccgcat ttaatgaacg gcgaaggatt ttttgtcggt gcactgagaa
    32701 aagggaccgg cagccaggga ccggttccca caactgctcc aaccggagtg gaatgggtaa
    32761 atcctccgcg ggaatcacaa aggtttattc catccggttc cggattgaaa tgttttcttc
    32821 accgggacca atattttgtt tttccctccg cgatggcgga tgccctggat caattcattg
    32881 gccggcttcg tttggtccag gcgggtacgg ccctgggcca attgaagaac aaggattttg
    32941 tgcctgccca cggctgggct cagagcctgg attgccccgg gacggtaatc tccttaaatg
    33001 tggaaaaagc ggaagcgctc cagtacctga gaaaggatcc tttacccagg cagggcctgg
    33061 accccggtta ccatctcatc cgttacgaag gaattagcct gggttggata aaaagtctcc
    33121 cgggccgcat caataatctt taccccatgg cctaccggat tatgcaggcc gggtgataat
    33181 aatcctgttg cgggcaggat tagtccggta actttattat tttggatcaa accggcatca
    33241 catgaaatta ttttcaggcg cccagatcag ggaatgggac aaaacgacgc tcgaggagga
    33301 aggcatccct tccatcgaac ttatggaaag agcctcctcc gccttcacaa agaagtttat
    33361 ggaacattac gaccggtcaa ggcctatcgc ggtcttctgc ggtccgggca ataacggggg
    33421 ggatggtttg gctatcgcac gcatgctcaa tacccagggt tatgaagtca aggtctggct
    33481 ggccgactgg ggcctgccca aaagtccgga ttacgaaata atgctcaagc ggctgcctgg
    33541 ctttggccag gttccggtct attctgtgga tatgaaggaa ccccctatcc ttggagaaga
    33601 cgacctcatc atcgatgcgc ttcttggcac ggggctgaac cggccactgg atggaaattt
    33661 tgaggacctg atttactggc tgaacagcct ccataagacc gtcgtttccg tcgaccttcc
    33721 ttcaggcatg tttacggatc aacatacgga gctggcgatc cgcgcaagcc ataccattac
    33781 tttccagact cccaaaatgg ctatgttgct accagagtat gaggaataca ccggcagctg
    33841 ggagatcgtg gatatcgggc tcagtcacag ctattatctg aagaccacgg taaaacatta
    33901 cctcgttgag aaaaaaatgt tgaaaggttt cctgaccacc cggaaaaagt tttcccataa
    33961 aggattgttt ggacatgccc tcctgatcaa cggttcacat ggaaaagggg gagcggcgat
    34021 cctcgcggcc aaagcctgcc tccggagcgg cgtgggattg gtcaccaatc atattccggg
    34081 ggccctctat gccatcatcc agatggccgt acctgaagcc atggcttcgg tggatgaaca
    34141 caattattat tggtcaaccc tgcctaaaga cctggataaa tatgcggcga tcggcctggg
    34201 ctgcggactt gatcagaagg attccacagc gcgggtcctc agccagttgt tcgatgaata
    34261 cgacggccgc atggtcatcg atgccgacgc actcaacctg atcagcaaat caaaggactt
    34321 gctcggaaaa ctgaatccgg gatccatcct taccccgcat ctcaaagaat ttgagcggct
    34381 ctttggaaaa agcataaact gttttgagcg tttatcctta ttgtccgaaa aagcaaaaca
    34441 ataccaggtg accatcattc tgaaaggagc cttcagttct gttgccaatc cggaagggac
    34501 catttttttc aacccttcag gtaatccggg gatggctacc gccggaagcg gcgatgtgct
    34561 caccggcctg ctgacgggcc tggtggcaca gggttatgat tccacccagg cggcgatcct
    34621 tggcacttat attcatggtg cagccgggga cctggccctg aaaaaacagg gaggagaatc
    34681 cctggtggcc tcagacatca tcaaacggtt gggagaagct ttcaaggact tgcgggcagc
    34741 atgaaaaaac tggtggtact gaccggggca ggcgtgtctg ctgaaagtgg cctcaagacc
    34801 ttccgcgatg ccgatggttt atgggaaggt catcgcgtgg aagaagtcgc tacaccggat
    34861 gcctgggagc gaaacccctc cctggtcctt caattttata atgaaagaag gcaacaactt
    34921 tcaaaagcct tgcccaacct ggcccaccgc ctgctggcag aattggaaaa acattttaaa
    34981 gtaagcatcg tcacgcaaaa cgtggacgat cttcatgaaa gggccggcag ttcccaggtc
    35041 atccacctcc acggagaatt aaagaaaatg aggagcgaac gatattccca tctggtctac
    35101 gattgcccgg gggacatcct gctgggagac ctttgcgaaa aaggatttca attgaggccc
    35161 cacatcgtct ggtttgggga gtccgtaccc ctgctggcgc tcgctgtacc ggaattcgaa
    35221 tccgcggata tctgcatcat tgtagggact tcgctacagg tttatccggc cgcgggcctg
    35281 atcggctacc ttcccacttc atgccgtacc tattatattg acccgcgccc ccatccttcc
    35341 tacgaaatcg gcttattgaa taacatcagc ctgatctcaa aaaaggctca ggaaggcatg
    35401 aaggaggtgt ttgaccggat tacgtcttaa ataagacagt taaaactggc tgatacggag
    35461 tacttatctt gtacacaccg gccagttgaa actggccgca acgggggcct gtacagacag
    35521 gccagttaaa actggccgct acaattactc aacaatcacc attttcttgc tgtccgtaaa
    35581 gaatcccgat tcaaaacggt agtacaggac tcccgtcgcc ccgatctcct tacggtcaaa
    35641 ccagacctcg tgatatcctt tggtccagtc tctttctata gaacggatga tccttccggt
    35701 cacatcgtaa atactcagcg tacccttccc ctctttcgcc agccgcaggc cgatcaccgt
    35761 tcgctggtca aaaggattgg gataattctg gtataattcg ctccttgcat ttacttcatt
    35821 cccttcaggc gttacaaacc ggatgccgag gttactcact tcacccttgc cttcgtaaga
    35881 ctctgcgata gtgacttgtg atcccatccg cagcgcttcg ctcagccgcc cgttctgtcc
    35941 ggctttgaag gtcagggtga ataatacctc ctggtccggg atatccacac ccgtatttga
    36001 attccagctc attgtgatca agccttcggc agcttttcgg tttccaaagt tttgatcgct
    36061 catgggcaat gcaccagtct ttggggtgcg gctgccatcc accacccacc gaccaccttc
    36121 cacctccacc gtaccctgga atccactgat ccctcggaaa ttaggggagc ggaattctac
    36181 aattaccagt tcaccggcaa taaattgctg gtctaccaca tcaaagatca aacctgggtt
    36241 atttcccctc acttcagtac ccataaggct atggggggcc gcgcttgcat taacatcacc
    36301 cgtcttgatc ccgatccagt cttcctgcat ataatctttc tccacctcct tcatttccat
    36361 ccaggtcttg aagtcccagg gattctttgg atcgctgaac tgctgggtct tcggcacaaa
    36421 cttccagcta tcggcattcg gcaactcttc gtaggtagat aatatcagtt ttctcagttc
    36481 caccaggtcg atggccgtca cctgcttgtc atcgtccgca tccgcggcga tgatcttgta
    36541 gggtgaatcc aggggttccg tgcccaggat gtgtttctgg atcagaatca ggtccagggt
    36601 gctcaccccg ttcatcggcc gatctttcct ctttgcccgt atcgtataat ccgatttgga
    36661 cggtaaagct tcaaagtggt aggtcccttg caccccggtt tcataattca attgcaggcc
    36721 tcccccattg atattcatcg tgacaaattc caccggctcc ttcccttccg tttgaacact
    36781 tccggcgatc tgaacattta ggcccgctac atccgggcag acgttgccgg tattgtcctg
    36841 caacaggata taagtactgc aatgatcctg gtttccggct tcgtccgtca cccagatctc
    36901 caccgggacc gtccgcgatc tgccgtccgg cagatcagca caggtgaata cccggctggt
    36961 ttgtgtaata tccgatgaaa aactgaatac cagattgtca tactccgtac aattgtcccc
    37021 gctacccgca tccaggtcct tcgcccacac cgtcaccgta cccgtactgg gcatgatcac
    37081 cgtggctaga ccccgataac agtatggcgt cggccgcttg caatccctca gttcaaaacg
    37141 gaaggtcgaa gtatcggcat tgccgcaatt gtctataccg ataatatgca ccaggtgttt
    37201 gcccggcgtg cccgacagga atataaattc ggtttcatac cgggtgcccc cctgatcaat
    37261 actgtttgca tcatacagcc gcccattata ggcatcctcg cttgcatcga tatcgatttg
    37321 aactcggaaa cggatgttgg aggggtcagc gcaattgtcc gatgcactta agggaatgat
    37381 cacatcagct gaacaatctt cattataaac gcatacgatt gtatcccgcg tattcacgat
    37441 cggggccacc ttgtccacca ccttgatgat ctggatgtat tgcatatacc cgtcgttatt
    37501 gtctttcagg ttccggtaca cgcattcccg gttacccgta tcggcccgaa gtctatcgtc
    37561 tacgatcacc tcaggatacc tttcttgcct tcccggatca taaatgcacc agtcgatcag
    37621 ggtccaggtc cgtacaatct tataacaagc gtccccttct atggtaaaga tttcatcctt
    37681 ataccgtata ccgacctgtt cacattcatc atccgtcacc tcaggctcac ccgtatcctc
    37741 cggttccgtg gaattcagga aatcacattc caccaccttg tcttccggga agaccacttc
    37801 aaaatcactg cggtgtttta ccaatacttt ttgtttggct accactacat tatcacactt
    37861 atcccggatg gtccaggtcc gctggatcca tccttccccg caggaattga tgtccccttc
    37921 gtccgtccag gtcagcgtgg cgtcattcag cgtgcaattt tccgtaatcg aaaactcgtt
    37981 tttcttcaat tcccgctgag gtcggttctt atcaaaaagc acgtaagtgt caaaataaga
    38041 tttaggatct atttgacctg aagtgtcaaa tgaatccagg tacagccatt cccgacaata
    38101 tttcggagcc cagttgttct ggtggtcata atgcccgcag tccgatccat gctcctcctc
    38161 atcatagtat cccaagcctc cataatagcc gtggatcgtg ccctcactgt ccttcagata
    38221 gcccggccag atcccgtttt tcttgcccgt ggagcagtct ttgggataac ccgcgtagta
    38281 gggagcatcg tcgcaataga ccgtaatgtc ctccagatgg tgtacttcag gagcctgttt
    38341 gtcgtccacc agcacctgca ccatgcagtc gctgtataat ctgtgggaac aataacttcc
    38401 ggggggattc gccgtcccgc cggtcagcat caccgtttct tcaaaataaa agtcactttc
    38461 gcagaatgca ttcaggttct ccaccgctcc ttcgtattga gcgtaacata aaccgccctc
    38521 tttggggtat gtgttatttc caaattcaca gatctcccgc cctctttgct gatgctcctt
    38581 ctgacattca attttatacc ggtagttgca gtcctttttg ccgtccttgt ggaaaaaatt
    38641 ccagttgtgc tcgatccggt agcgctggtc ggtattgtac cagaaccact gatgttccgt
    38701 gcagggccat acatgcggat cgtatattgg tactccgcag gcttcataca ccctcaatac
    38761 cacctggttg ggaccgcaat catcaaattt gatctcatcc ttaaacacgt aggcgttgat
    38821 ccatctttcg atctggaatt cataaaattc cttgtctttc tcatatttgt agctgccgca
    38881 gcgggcttcc agggaatccc tccagtaatt tctccaatag gtcacgctgt ccatgtgggc
    38941 cactgcaaaa tgcaggactt cacaacaatt gtcccggctt ccgttgtcca ggtctttggc
    39001 tttcaccttc gcccagcagc cttccgggtc cacggttacc tgggtcacct cgtcgcatac
    39061 cggggtcgga ggcgtgatgt ctttaatcac gatcaaccta ctggtttccg tccggttata
    39121 acagtcatcc accaggaaga tctgaaccat atgttccccc tgcggtaagt acagggtctt
    39181 ggacgggagg tcacccgcca atacaatgac tttacccggg tggctcttat cctcgtaagt
    39241 gattctatag gattgtttca ccccgctgca gtctttataa tcggtacctg ctaccagggc
    39301 cggaaccact acacctgcca cacattcgtg ggcattggta tttttataga tcgtcgccag
    39361 gctctttagc atcctggggc cggtcgtatc gatgaccttg atgtattgtt cgcattcttc
    39421 ctcttccagc gtgcaccagt ccgtcacgat ccaattgcgg tacaccttga agcccgctcc
    39481 gcagacatcc agatcggtat ccttgtatat cgccgtgatc ttgcagctgc ctcccgctgc
    39541 cggatatata ttcttccctt cgatctgggg caccactatg atcgcaggat caaggatagc
    39601 tcctctaccc cctgctttta attcccagtt ggctttctgc aatgaaagca ggtattccgg
    39661 agatactttg gacttgtcga ccgtgaaggg ggtcttgagt tcggatttgg atccatacaa
    39721 ttgatgggtg atctcgcagg gcagttcgat cagttccggg cagaccaggc tgtccaggct
    39781 cgtcttgcgg ataaagatcg tatccgtgca acgtgcgaaa ttgccccatt tgtccgaacc
    39841 ctggatcgtc cgcaccacat aacccgacaa gaccgggtct tcgtcgcaac catattctgt
    39901 ccaatggtcc gaaacgattt ttacttccac cccggagcag ctgtcttctc cctccgccaa
    39961 caaggggagg tcaggcagtt cgtaacaggt aatcgtatcc accgaacaat tcagctgggg
    40021 cggctgcttg tcctccacat ggatatagcc ccagcatttg ttgccggtag atacttcata
    40081 tgcattgtag accagggtat aaccgatatg gctcaggtct actttatcgg cctccggata
    40141 tcttgccgta ccaaaaggat aaatcaactg gatatcccaa taattgcggc aacccagcac
    40201 atcttccagg acgtcatccg ggtcgatcac tttcatgcaa ctggtaccca gcgaaacatt
    40261 caccaggtca ttgcagacca ggtccttggt aaaggtatcg agcacccata ccattttggt
    40321 acaactgtcc accagacaat tgcgcacaat ttccagcagg agattgtttt taccgagctg
    40381 ttcgcagtcc aaaacgatgt cgcctgtggt ctcaattcca ttgactaaaa acgttgtgcc
    40441 atccggcagt tcacccagga gtacggaatc cagcggcagc accggtaccg tcccatccgc
    40501 attgacatat atggtatcat tgaggcaatt taaataagct tcataggggc cgatatcgat
    40561 ggttccctgg gcgatccggg gcagccaggc aatgtcagta ggatacgggt tatcagcatt
    40621 attccccata ttcactaatg gagaattata tggaaaacgg aaatttccta ccggggttgg
    40681 tgctgtattt ccgagaggtg cattaaagaa caaaggatca aagccttcgc ttccaaattt
    40741 aagattcggt ccagtcataa tggtaaaggt attaccccca ttatcacgcg tgatagagga
    40801 atgggtaagg tcccccggag ggccgcctcc accctgaatc aggctgtaat cgatatccaa
    40861 ggttccgtcc tgaatccaaa ataccggacc ccgattgggc tgggtggcaa tattattcca
    40921 gaaagcagaa ttccggatga aaagattgag atccccaccg ctggctccat tattgaatgt
    40981 ggctccccct tgtcttgcct ggttgccgtt gaaagtgctg ttgatgacca tagggtggga
    41041 tgtcgctgta ttatcatcac catttccttc cgcgtcatta tacattgcac ctccgaaatc
    41101 ggtcgccagg ttaccgctca gaatacagtt gatcagggta ggactgcttt cataatttag
    41161 cccataatta aacatacccc ctccgcggga ggcttcatta tcactgatta tacagttgat
    41221 taaagtgggt aaagttggtc ccatatcacc gaaattagcc attccccctc ccctcaaagc
    41281 agcatttcct gtgatcgtac aattcagaat ggttggattg gacgaatccg gagccgtggc
    41341 aaaattaaac cagcctcccc cataccggtt gtattcattc ggcggtgccg atgcatatcc
    41401 atcctgaatg caaaatccgt ccactaccgt tgcatcgctc acattatgcg tcgtgacgac
    41461 atgataactg ttgttcgtcg aatcatcatc ctgctggatc tctccgctta aaatggtttt
    41521 atagcaccag ggatcacgtt ccccattccc gccggcaggg aatcccccca tgactaccgc
    41581 tgaatccgga atgttgaaag aggtatccct tcctgtgaca gcaggataat aagtgccggc
    41641 agccacacag atggtgtcca caaagcaggc attgagcgca acttgcaggt cggtaaaagc
    41701 atcctcccag gataagccgg tatttgcccc ggtagccgtc tgatcaacat atattttcct
    41761 cgggaaggag caatgaattt ttaccaggac ctcatcaata cgagctgaag ccaagggcgg
    41821 gagaatgggt gcattatccg ccagccttac ctgtatgcgg accccgaaac cagggtcatt
    41881 gatcatggcc ggggtccagt tgactcccca gttgtcccct ccataagaaa cccgttgatc
    41941 acccgtaaaa taggttatca agttaactac gcttgccgtc gagccgggcc agttggtggc
    42001 ggtatttgcc ttattggctg actccgctcc gccaggttgt aataaagcaa ttttaacatc
    42061 ggtgaccgta tcttgaccaa caacctgctg taaaatatca aatccccttg cccggtcaac
    42121 ctcaacggta atactgtcaa taatggcgtt gggacaaatg caaaaaccaa aatccgtgag
    42181 gtccaggcag gctgaggtat cgactgcatc tccaatatta ttagtaaccg tggcatatcc
    42241 tgtataaata ctggggacta aaacgatcaa accatcatcg gatgccagat tagcagcggt
    42301 ggctcccgtc cagctggctg aaccatcgct cgaagggcat ccgaacccca ccgtggaggc
    42361 aacactactt tttttcggag tgacataggt cactgcaatc gatacatagt caacccttgc
    42421 tgtgatagtg ccagaagccc tgttccggtt attttgaatg cggacaccaa ataagggcga
    42481 attaatatcc gccggggtcc aggacgggta tgcccaagaa tcagagctgc ttccataggt
    42541 aatggtggaa tcggtggttg accaggtaat ggataaggcc ttattttgag cttcggtgtt
    42601 gcttgcaccc cggatcaggg taattttatt gtccgtgaca ttgttggtag ccgtcctgac
    42661 gtttgcactt ctttctaccg tgacagtgat ccctaatatt tcagctccgg aaggaatgga
    42721 aaatccaagg aattgaagat ccaggcagtt ggaaacggcc gaccctatcg ccaatacacg
    42781 agaatttgta gcataagcat catcactggc cgtcgtgttg ccagccatca gccatggggc
    42841 cgctaggtta caagctcctt gttcaatgtc atctccttcc actctgacat ccgtgatggt
    42901 atctcctccc atccctgcat tcaggagggt cggggcttct tcaaaggaat atggagaaag
    42961 ttgaagtgga ggattggaaa tcaagtccgg ttttgaaagg actttaaaag tgaaataacc
    43021 ggtcaaaatc aaactcagaa ggcggacaga aataaaaggc ctcctgacaa agttggtcag
    43081 gcgaatgatc atgtgtaaag gtttggtaag ttgaagataa caagccccct tgaaataaaa
    43141 aaattaagtt aaaaatatta tatcagctaa ttctcaacga ctttgactat gtatcgcgta
    43201 attctccgct attatgaaga acgactgtgc gctaagtcca tatttatgaa ataccactct
    43261 gtatgggtga ctgaatgcga cgttccgggg tatttctgct ccacaggggc cactgaattc
    43321 gccactatgt agaatgctca acatggcgac tgaagacgca gatacggaga ataactctgt
    43381 ggtagcgact gaggtcgccg ctaccggaaa gtactcaaat atggcgactg aattcgccgc
    43441 tactgggatc tactccagga ctaccatttt cttactatcc gtaaagaagc ctgattcaaa
    43501 acgatagtac aggactcccg tcgccccgat ctccttacgg tcaaaccgga cctcatgata
    43561 tcctttggtc caatcctttt ctatggagcg gagggtcctg ccggtcacgt catggatgct
    43621 cagggtaccc ctgccctctt tggccagccg gaaaccgatc aacgtacgct ggtcaaaagg
    43681 attgggataa ttctggaata actcgctctt tgcgtttact tcagttcctt caggcgttac
    43741 aaacccggaa ccgaaaagcc gtccccaaat cctctcttta ctttggaaga acttccacgg
    43801 tcctcccagg gacctgtgca tacaccgaag cgtcgtacat ttcctcgcag acgatggccg
    43861 gaagatggaa acggccttca taggtggccc tggcctccag ggtaaaggtc ttggacttgc
    43921 cgggctcaag ttggaaaaaa gtaaacacgc ggtcatcccg gatgtcctgg taatcgaatt
    43981 cctgtccacc ggccgtcatt ccgctaagtc tttgattgat gatctcccag ccggagggca
    44041 ggatatgatt cagagccaga tgggcaaatg gtcttggcct ggaaccggta tgggtcacgg
    44101 taagttccac caggatgaga tcccccttct tcagttggcc gggatcgatg gcttgcctct
    44161 cgggcgaccg gtaacgtaca ttcatcgtca ggttattgga gatcgggcgt tcgggggcct
    44221 gctccggctg acccacctgg accgactgca gatagatcgg ctgcgtagag gtattttcga
    44281 tcgttacgga acctggcatt ttgccatcgc ggattgggtc gatcaggatg gaagcattcc
    44341 ttgagtgtat attcgacttg gctcctccat catagctgta tttgaaactc caggtggttc
    44401 cgtccgaagc agcccccgta tgaaatttac ccagggcctg aagggcatag gccagggatt
    44461 gggtattcca gggtttttcc aggttgagtt gtttgttcag gtccagggcc atggaggcgg
    44521 ctttggtacg gtctccgatt tccagccagg tctcgagggc catggccagg tcgcggaggg
    44581 cactgccgta ggtaaatccg gattccgaat actcttctgc tacgaaggga atctgcacaa
    44641 gggtcctggc cgcttcggtt tgcccggcca ccgcataagc tgcggccagc ctccaccggg
    44701 cgttattgcc cagttctttg tattccttga gccggttcat cgctccgaga tccggggtgc
    44761 cggaaagggc cagggtatac aaacggtacg cctggtccag gccatgattg gccttataaa
    44821 aggctttctg ggcaggatcc catagcccgg ccagtttttt ctggtgtgct ttccagttgc
    44881 tcatcaaata ttggggaacc gcatagcctt ttttctccgc ttcagtgagg aaatgaccgg
    44941 cataactggt tacccaggga tcactggcat aagcctgccc gggccacagg ctgaatgaac
    45001 catcctggag actgaatctt gaaagggaag tgatcgcgct ttcaatattt attttcagtt
    45061 cccggctttg attttcattc agggtgacat attgatccag gtataattgg gggaaggcgg
    45121 cagaaatgat ttgttccgcg caaccgtaag ggtagcggac caggtagtcc agatgtttgc
    45181 ccagggatac cggttggagg gtggctattt caaggctcgc cttttgagta gtgaccgcat
    45241 cctgcgcctg gaaggcaaga gtcttcgatt gtccgggttc aatcacttca ttcatcaccg
    45301 ttgaaaccat gggattggga ttacggattt caatttcgat gtcctgtttg gcggattctt
    45361 ttccggattt tgcctcgatc cggatcgtgg tctcaccggt tcggccatcc gtgttgaatg
    45421 aaaagtattc caggcgctcc ccttcccctc cggcaggcag ggtcttcgag ggatcgtccc
    45481 atttgagccg tttgtttttg tcttgagccg taagggtgat gggctgggta atgtctttcg
    45541 aggtaaacag gttgacgggc agggtcacct gttctcccgt acttaaaatc ctgggtaagg
    45601 tggggagaat cataagcgga ctgatcaccg gcaaggtttt ctctgccttg ccgtatgccc
    45661 cttctgccgc ggccaccacc atgaccctta ctgagccgat gtagttatct atattaatgt
    45721 tgtgtttttt cttctcccct tttttaagtt gaaacggccc caaatggatc acgggagatt
    45781 tgaacctgct gacgaccttg gagtcattca gtttcgcgcc gccttcatcc ccgccaatgc
    45841 ttagaatgct ctcaatcctt cccccaaagg cgccgagcac ctgatcgtag atatcccagg
    45901 tgcgcacccc cagcgcttct ttcgcaaaaa aatgaccgtg gggatccggg gttttgtggt
    45961 tggtgaggtc caggagccct tcatccacga tatcaagggt ataggccatg ggccgtcctt
    46021 ccgtctctga aacttcaatg ctttcgttcc ggtccggacg caaagaggcg gaagagttga
    46081 tgaccggctt caggagcgtt gaaggatggt caaaatagac cgggatcacc ccatacatcc
    46141 gcataggcag gtcatttccg gaatccccat gttgctgcaa aagggatacc tgtacgtaaa
    46201 cggccgggga catctccggt tttggtttaa accggaagat aaacgggctt ttatccgcat
    46261 tggcccaatg ggattccaaa acctggctgc cgttttcaat ggtgatcagc atccgggagc
    46321 ccagaggggc ggggatgttg atctctacct cttcatccgc caggtatttt tcttttgagg
    46381 cctgcaaccg gagcagggtg gccatatcaa aactcctgga ttcatcttcc ggccagccgg
    46441 cgtaagcgaa atctccactg caatggccgg agaccggatc acaaacccgg atgagatacc
    46501 ttccccattg attgacggtc accttccatt cggccaatcc gttggcgttg gtcgtgaggt
    46561 ttgttttttg tatggctttc aaatggtcgc tgctgttgta ggcggcatac gatcccccat
    46621 actcttccca ccaccagcgc cagtccatct gatagagccc cacagtcagt ttccttcccg
    46681 cctgggcttt gccggccgga tcaacggaaa caaactgaat ggtggaggat ttgcctttat
    46741 ccagggtccg gtaaccccag ggatcctgcg gcagcctgat cccggcatat tgaaggtatg
    46801 gatgataaag ggcggaatgc gtgaaggtgc tgaaatcgcc tcccggttca gaaacttcaa
    46861 ttttaaactg gagattcatc ctgcccgccg gtttaaaatc tttggaaatt ccaagcggga
    46921 tggatgtacc gccctcctca tccaggggac cgtcgaaaag ggtgatcacc gggttttcat
    46981 tgctgatcct ggccgggtcc tggaacacga attctttgta tgcaggccag gagccggagg
    47041 agggcctcca ttgagtggtg accattgctt ttaatccgct tgccttgagg ccggtgagcc
    47101 actgggcctt caaaacggcg gtctgttgac tgggtgtgat ttcttctccc acatcccaat
    47161 tgatcttcag ccggttgggt tttatacttt caattttaag gattttgttg aattccactc
    47221 cgccggcttt aaccaccgcg cgccaattgc ccgtgatggc atcctgttcg gttttaaaag
    47281 gaaaagaata aaaaccctgg atgtggtcgc tggaaatttt ctggatgact ttacggttct
    47341 gcgcatcaaa tacctgaagg gataccggat ggccttccgg gaggaggtgc tctttctgat
    47401 tgagtaaaaa actgaagtgg atgctgtccc ctggcctcca cacccccctt tcgcaaaaca
    47461 ccaatccttt gaggcccttt tttacatcct ctccgtccac ttcaaaagcg ctcatggaaa
    47521 gattgtgccc gtcagccatt ctaaggtagc ctttctggcc ctgaaaggtg gctaccaggg
    47581 catagggtgg ttcggtcagc aactggttca ccaggccgtt ctgatccgtg gtccactggg
    47641 cgattttttc caaagtaaag ttatatactt caatgctgca gcctgccagg gcttccgcgg
    47701 tgcgcaggtc cgtaacggct gcgaagaggt gtttatccga ttttttacca atgatgccca
    47761 ggttggaaat caggacattt ttggagatga aatgttcgtt attgtagtag gccgctttgc
    47821 agggatcgtt gcggtcttcc cattcgtatc cttcatagta tccttcgggc ccgtaaaaat
    47881 tatcttcata aaagctgatc agttcatctc cggggtccgg gccgggctta tcctcgaagc
    47941 ccgccatgca attgaggtga gtataagaag gctggaatcc aatccggacc tggtagatgg
    48001 cgttggggtc cgcttccagc aggggttgca gatcaagagc ataatgggac cagttttcag
    48061 ggtcggcacg gggatttaca gttgccagat cgatcctgga ctggcggatc agtttcccca
    48121 cccggtataa ttcttgttcg tcttccagtt gattaacctg gagaaattga aggatattct
    48181 gatcaaagat tttaaatact tccagatcga tggctttcag accgaccgct tcaaatggca
    48241 ggatgacctc atcgtgttga ggaatgatgg taccgcgacc cgtaaaccgg accgcaggat
    48301 ctgtggccgg gatctggatc tcccattccg aactttcctg caattttttg cggcctgaat
    48361 cctggatacc cggggaaatc aggacggttt gctccccttt caaaccccga ggtaggtatg
    48421 cataaatctt atggccttcg gtcatggttc gcagagggcc ggcctggttt ttcagataga
    48481 tcagtccggt caggttctgg ttagcgagga ggggatcaga aaaagaaatg acgatccggg
    48541 attcagggct tttttcaaaa aaaacatcaa ggatcataaa ttctcccggt gccgggatcc
    48601 ggatctcgta tttccctttt ttggagatat ccgccggggc cccgctccag ttcaactcaa
    48661 gcctggaatc cttgatcctt ttgatgtcat gaaatgtaaa agtgctgaca ttggaaaagg
    48721 tatcgtgctg ccagcttgca cgcagggctt gccggtcctg gctggccatg agtaattttt
    48781 caaccaggcc gggttccata aaatcggaag tgctcagggt gccggtaagt gtatagaaat
    48841 taagttcccc ggaagcaggt tgaagcattg gctttgccac ggcaaaactg tgttcacggg
    48901 tcctgacgct gtaactcagc atctccggtc cctgcatgcc ccggggcaga atggatttca
    48961 ggtcaatttt tatttcatac cgggtacctg gtttgagcgg accggccggt ttatagatca
    49021 gggtattcct ggattcccaa taggcctttc ccggtgcggc agggatgatt ttgaccatgc
    49081 cttccgcaac cgggctgccg acctcttctt gctccaccat atcctgggta aacacataac
    49141 gcaaagggga cttcgatgaa atgatcccct gggtgtagga ccgcaatacc gttcccaggg
    49201 actcctggct ggtctccggg gaagggccct gcttacaata taaaaggaag aagggggcgc
    49261 cggcgaggag caataaccgg atggatggat tcatggaagg ctggttttat tttctttggt
    49321 aaagctatgg atatacccgg tacccgggtc aatttaaaaa ggaatatttt ggaagtcggc
    49381 cctgggttcc cggagggcca ggaagtcaat aacccggcaa agtctagcga tttggtagag
    49441 aatattaata ccttcttaac tttttagaaa agacaagggc tggtgaattt cttcgggccg
    49501 gtttattttt ggtattatct ttgtttcgcg ttaaatcaaa taatgagaag gatacggcac
    49561 gacgatttat tcaagcacgc tcacgggtgt tgttgttgct gttgttgccg ttgattgcaa
    49621 ccgccttctt catttccctc attcattcca aaaatcaata aggacggtaa aaaaaatata
    49681 gccagagccg gcttaaagag tacatttaga aataaacatg gaagctaacc ctaaacagat
    49741 cgaatcgctc accggcctgc tctccggcca ggaagacctc cggctagccc ttcaactggc
    49801 ttgccggacc ttcagcggac gggcggtctt ttcgaccagc ttcagcgaag aggatcagat
    49861 catttttcat tatctcaatg aaatagacca cccggccagc acctttaccc tggacaccgg
    49921 gcgcctgttc ccggaaacct attccacctg gtccaggacc ctcgagaggt ataaactgcc
    49981 catccgctcc tttcaccccg atgcaacggc actgcaggcc tttatcgaaa gccgcggacc
    50041 caattcattt tacgaatcgg tggaaaaccg gaaaacctgc tgtcatatcc ggaaggtgga
    50101 accccttaaa aaggcgctgc agggccaggc gctctggatc accgggatcc gggcagccca
    50161 ttccccggac cggaacgata tgaccccggt ggaatgggat gaagccaacc ggatcatcaa
    50221 ataccatccc ctgctccact ggacccatga acaggtaaaa gcctgcatcg cggaaaacca
    50281 aattccctac aacccgcttc acgacaaagg attcgtatcc atcggttgtg caccctgcac
    50341 ccgcgcgatc cggcccgggg aggatttccg tgcgggacgc tggtggtggg aaacaaataa
    50401 aaataaagaa tgcggtttgc atgtccatgc cgatccgtcc aaaccatcca taacatgagc
    50461 gactaccgta tcgatcatct caaagaactg gaagctgaaa gcatttacat catgcgggaa
    50521 acggctgccc agtttgaaag gccggccctc ctgttcagcg ggggcaaaga ttccatcacc
    50581 atggtctacc tggcgatcaa ggcctttagc cctgcgccca taccctttcc cctggtacat
    50641 attgataccg ggcacaattt tcaggaagcc ctcgactacc gggatgccct ggctgcccgc
    50701 acccggtcgc gcctgatcgt ccgcaaagtg gaagatacca tccgcgaaaa aaacctcacc
    50761 gaacccaggg gcaagtttgc cagccgcaac tggttgcaga cccacaccct tctcgatacg
    50821 atcgaagaat ttaaatttga cgcctgtctt ggaggcgccc gcagggatga agaaaaggcc
    50881 cgggccaaag aaaggatctt ctcggtccgc gatgaatttg gccaatggaa tcccaaactt
    50941 caaaggcctg aattgtggaa tatcttcaac ggacggatcc ataagggaga aaatgtccgg
    51001 gtcttcccga tcagcaactg gaccgaaatt gatgtctgga attatatccg gcaggaaaac
    51061 atacccctgc cgaccatcta tttcagccac gaacgcgaag tgatttccca cgagggtcag
    51121 ctcatcgccg tctccccttt cattaccctg gaagaatcgg atacccgtct gatcaaaaaa
    51181 gtaaggtacc gcaccgtggg agacatgacc tgtaccgcgg ccgtcgagtc ccaggctaag
    51241 acgctcaatg aagtgatcga agagatcctt tccagccgga tttcggaacg gggtgaaacc
    51301 cgcatcgacg acaaacagac cgaagccgcc atggaagacc gtaaaatcaa cggttatttt
    51361 tagaaacccg accaatccac agcaagtatg gaattactca gattgatcac ggcgggcagt
    51421 gtggatgacg gtaaaagcac cctcatcggc cgcctgctct atgacagcaa aaacatctgg
    51481 atagaccaga tcgaacaact tgaaaagatc tccaaaacaa ggaaaaccac ccagatcgat
    51541 ctggccttgc tcaccgacgg gctccgtgcg gaaagggaac aggggatcac cattgatgtg
    51601 gcctaccgtt acttttccag tccgcggaga aaatttatca tagccgatgc cccgggccat
    51661 atccagtata cgcggaacat ggtcaccggt gcatccaatt ccgaactcat gatcctcctc
    51721 atcgatgcga ggcagggggt catagaacaa acccgccggc attccatcat tgcctccctc
    51781 ctgaagatcc cgcatatcgt ggtcacgatc aataaaatgg acctggtgga ctattcggaa
    51841 aaccgttacc tcgaaatcat ccgggattac caggaggtgg cttcacgcct tgggcttcct
    51901 gacgtacact ttattcccat atctgccctg ggcggagaca acgtggtgaa tcattcctca
    51961 aacatgccct ggtacgaggg ccccgccctg ctgcccctgc tcgaagaaat agaaatccgg
    52021 cgcggggata ccctggaaca atcccggttt caggttcaat tcgtcctccg tccgcagacc
    52081 gatgatttac acgattaccg gggctatgcc ggccaggtat ccggtgaggc ctttaaaaaa
    52141 ggggatgcgg tcagggtcct gccccagggc ctggccagca gaatcgccaa actcgaacat
    52201 catggcaaag aggtggaaca ggcagaaccc ggtcaccctg cggtgatcca tcttgaagat
    52261 gatctggata tcggccgggg cgatctcatc gtaagagcgg gcgaagaacc attggtcact
    52321 aaaagccacg aggcggtcat ctgctggatg gatgaaaaac ccctggtcac cggcaataaa
    52381 tacctgcttc aacagcacag cagactgatc aagaccgcgg tccgggaaat tgcataccgg
    52441 attaatgtca ataccctgga tcacgaaacc gggattgaaa aactggaact caaccaggtg
    52501 gcccacatcc gccttcagac ggcgggacca ctggcttatg atacctacga acaatccaaa
    52561 gccaccggca gcgcaatcct gatcgatgag accagcaacc gcaccgtggc agccgtcctc
    52621 tttttacatc cccaggccgt ggattcaacc cattaaaacc atggacaaac ccattccaaa
    52681 aataattctt gccggggcag gcccagggga tccggacctc attaccgtca agctggccga
    52741 agcccttaaa accgcggagg tcatcctggt ggaccggctg gtaaaccccg aaatattaaa
    52801 aaaatatgct tccggccagg ccagtatcct gtatgtggga aagcagggat attcgggaaa
    52861 ttcctttccc cagcatgaga tcaacaggct cctgacagaa tatgctctag ccggcaaaaa
    52921 agtattgcgg ctcaaaggag gggatgtatc ggtgtacagc aatgcttttg atgagataca
    52981 aactctacaa aaccactcga tttcctatga gatcatcccg ggcattaccg cggcttcagg
    53041 ggccgcggcc agtctgggca tacccctgac cgccagaaac ctgtctcccg gcctccggat
    53101 cctaagcctg tcagccggac aagagccggg ccccggtgaa tttaatgcct gggccaaatc
    53161 tccggaaacc ctggcctttt atatgtccat tgaaaacctg ccatccctga tcaacggatt
    53221 gttggaggcc ggcgccgatc cggaaatgcc actcgccctg attgaaagag cgacccttga
    53281 aaatcaaaac agcgcggtct atcccctggg gcggtttctg actgaaacgg caaaccagtc
    53341 cttcgcttcg ccgaccctgg tcctgatcgg cagaatcctg aaccatattc agcctggctc
    53401 caccccttcc tccaccggaa aggaaatcac caagcccgaa tccaatcccc caaccttaaa
    53461 taattttttg catgttatct gaaggaaggc tacgaacctt aaacgatttt atcgaaaaga
    53521 cgaccaagga agaactgatc tggatcaacg gctatattgc aggactcatc tcctccgcag
    53581 gcccttccgt tgcggagccg gcccagccat ccaaaaagtt tacaatcctt tatgggaccg
    53641 ataccggcaa ttcgaaaaaa ctagcccttg aattcaccgc ccttgccaag aaacaaggcg
    53701 gcaaagtcaa agtcagcggc atggacgctt ataagcctga agacctggcc caggaaaaac
    53761 aactctgcat catcatgagc acccatggcg acggagatcc ccctcctgcg gcaaaaaaat
    53821 tctatgacca cctcatggaa accggcaccc aataccccaa tcttgagttt gccttactgg
    53881 ccctgggcga ttcgtcctat ccgctttttt gcaaagccgg cgaagaggta cacaaccggc
    53941 ttttggcatt gggggccacc tcggtctttc cgctccaaaa atgcgatatc gattaccagg
    54001 aggaagcaaa caattggttt cagctttacc tgaattcctt cccctcggct gccccggccc
    54061 cccctgtccc accgaaaaaa gcgccccaca cgaaaaaatg gtactccggg gtcatcaaaa
    54121 ccaatatcaa cctcaacgga catacttcct ccaaggaaac gcaccacctg gagatccgta
    54181 cccttgaacc ggtactctat gaacccggcg atgcgctcgg ggtgattccc aaaaacagga
    54241 agtccactgt ggatgaaatt ttatccctgt tgcaggtgga ttccaacctg agcgtcccct
    54301 ataaaggatc tgcttatacc ctttccaacc tgcttaccca aaccttcaac atctgtcatc
    54361 tgaaagaatc ccaggtgcag aaatacgcgg acctgaccgg tcatgaaatc cccaaagtga
    54421 ggatggacct gctggattta ttgaaaatat atccggttcg gaacagcagg cagtttatag
    54481 aattcaccgc tcacctcacc gaacaattgc ccaggctgta ttccatctgt tcatcccccc
    54541 aggcccgtga attcgaaata cacatcctgg tcggcaaaaa ttccttcgag gcccaaggtg
    54601 aaaaccggtt tggactttgc agtgaacaac tgaacgaact gtacgaagga accccggttg
    54661 aattctatat ccataaaaac aaatccttcc gcctcccttc cccggataca gacgtcatcc
    54721 tgatcggccc cggtacaggc attgccccca tgcgggcttt tattgaggac cgcgacgcct
    54781 caggcgcttc aggaaggaac tggctcattt ttggcgaaca acattttgtc tccgattttt
    54841 attaccagac cgagattcag cagtatgcca gcaccggcat ccttcaccag gttgatctgg
    54901 cctggtcccg ggaccagaaa gaaaaacagt atgtccagca ccgcatgcgc gaaaaaggag
    54961 aagaacttta cgaatggctc aggggcgggg cccatgtatt tgtcagcggg acgcgaagcc
    55021 cgatgagcga ggatgtcgag aaagcctgga tcgacattat ttacacccac agcgggaaaa
    55081 gcgctcccga atcggaggcc tggtggaatg agatcaaaga atccggacaa tacaaggccg
    55141 atgtttattg ataaacccat aaaccgacaa tcatgagcaa tccaaaaacc ggtgaaccca
    55201 acctgagcca ggtagaaaaa ataaaaatga aaagcgacgg cctgcgcggc agcctggcgg
    55261 agagccttca ggaccagctc accggcgccc ttcgggaaga agaccagtcc ctgatcaaac
    55321 atcacggcat gtaccagcag gatgaccggg acctccgcga aagccgggcc cagaaaaaac
    55381 tggaatggtt gtattcctat atgatccgcc tccggcttcc aggcggtttt ttaaacccct
    55441 cccaatgggt ggccctgcat cacattgcag gagaacacag cacctgcgtg atcaaaatca
    55501 ccacccgtca aaccatacag cttcacggca ttttaaaatc tcacctcaaa cctaccttga
    55561 aggcttttgc cacccaaagg ctggacagca tcgcggcttg cggggacgtc aaccgcaatg
    55621 tgatctgcag cgcccacccc aaacaaagcc gggcgcacga agaagttttt gcctatgccg
    55681 ataaaatcag caccctcgcc cttcccagga ccaatgccta ttatgagatc tggctcgacc
    55741 gtgaagcgct tacgaataaa gaagaggaga tcgaccccct gtaccaggac cggtatctgc
    55801 cccggaaatt taaaatcgca atctgcatcc cgcccaacaa cgacgtcgat gtgtttgcca
    55861 acgatatcgg cctgatcgcc atcctcgatg gggacaaact cctgggattc aacgtggcca
    55921 tcggcggggg actggggacg acccatggca atgcggacac ctatcccagg ctggccacgg
    55981 tcataggctt tgtggcctcc gaagaaaaga tcctgaaggc cgtctatgag atcatcacgg
    56041 tacaacggga ttttggcaac cgcagcgacc gcaagcaagc ccggctgaaa tataccatcg
    56101 accgcatggg tctggacgcg ttcaagtcgg aagtggaaaa aagatgcgga tttgaattcg
    56161 agcccgcgaa gcccttttcc ttctctgaac gtacggacta cttcggctgg catcagaatc
    56221 atgaaggcaa ctggtattat accctcttcg tcgagaacgg aaggattgcg gatgaaaacc
    56281 gggccataaa aaccgcttta tatgaggcgg ctcagactgg aaaaaccaat ttcaggttta
    56341 ccgccaatca gaaagtcatc ctcagcgata tccatccctc cgacaagaaa gcgatcgaac
    56401 aaatcctgga aaaatacgac ctcatcaagc atacggatga agccatcccc atgcgtcaga
    56461 atgccatagc ctgtgtagct ctgaatacct gccccctggc actggctgaa ggccaacggt
    56521 acctccccac cctcatcagc aaactggagc ccctcctgaa aaaatataaa ctcgaaaatg
    56581 atccgatatc catccgcatg accggttgtc caaacggttg cgggcgcccc tacctggcgg
    56641 aaatcggttt gatcggcacc tcctacggtc gttacaacct gcatcttggc ggcgaccgcc
    56701 ttggtgaaag actgaatgaa aaaaaatacg agcaggtgaa tgaggaggag attataagca
    56761 tcctggatcc actctttaaa aaatatgcag cggccaaaaa gaaataccaa tcttttgggg
    56821 acttcattca ccaaaacccg gaactggtca ccaccagata agagccatgc ctgtgaaccc
    56881 ttcccatctt tccgatcctt tagccccctt tgattaccag ggctgggccg aaaacacatt
    56941 gcagggcctg aaattggagg gcgcttaccg gtattttttg ggcattgaaa agcgtgcgga
    57001 aacctatcca caattcgact ttacggactc ttcaggacag accaaatcag ccattaattg
    57061 gtgcggcaat gattacctgg ccatgagcac acaggaagct gtgctgaagg tcatgcataa
    57121 aaacatcgcg ctggcaggtg ccggcagcgg aggcacgcgc aatattgccg gaaccacccg
    57181 cctccaccag gaacttgagc aggaactggc ttcctggaat gggaaagaag ccgcactgct
    57241 gtttaatggt gcctatcagg ccaatcaaac caccatgacc accctgggca ggctcttgcc
    57301 ggaccttgtt tttatttcgg acgaagaaaa ccatgcctcc ctgattgaag gaatgcgtgc
    57361 agtgccaaat aaaaaataca ttttcaggca caatgattta aaccacctgg aggaaatcct
    57421 ccgggccctg ccgccggcgg caccgcgctt gctggtgttc gaatcggtct acagcatgtc
    57481 gggcacgatc gccccggtcg ccgaaatcgt cgccctggca aaaaaatatc agtccctcac
    57541 ctacatcgat gaagtgcatg cggcaggcct ctatgggccc gaaggttcag ggctttcgga
    57601 agaactgggc gcccgggcgg aaattgattt tatcaacggg accctttcca aggcggtcgg
    57661 agtttttggc ggatatattg ccgcttccgc cctatgggtg gatttgatcc ggagctttgg
    57721 accgggattt atttttacaa cctccctgcc cccggccctg tgcgcggcag ccgctgccag
    57781 catccggtat atccgggaac acccggagat caggctggcc ttttttgaaa atgtgcacct
    57841 cctgagatca catcttgacc atcatgccat cccttattcc ggaaaccaga gccatatcac
    57901 ccatatccga atcggcccgg aagatctctg caaacagcgc agtgaaaccc ttttgcattc
    57961 ttttggccac tatcttcagc cgatcaattt tccaacggtg cccaaaggag cagcctgtct
    58021 gcgcctgacc atcacaccca ggcatacgga agagcatatt ctccggttga ccgaagccct
    58081 ggaccaggtc ctggaccgga cgatcgtgct gacgggaaga ggatccaaat tgtcccgatt
    58141 gcaaatcgat atcgctaaga ataaaattca agacgctttc cccaggctca gggttgaaac
    58201 aaaatataaa gacagtctgg gtgatcagtt gagcgacatt cccctcgata cccaggaagg
    58261 cgttgatttt ttcacccggg tattcgcgga cgatttacag gcaggaaggg ctgatatcgc
    58321 ggtgcactcc ctgaaggacc tgagcggcga tcactttttc ggatcaaacc gttttgcagt
    58381 cattgaccgc gaggagaccc gggatatagc cattttcaac ggggatgtcg gagaaaggtt
    58441 gcgccaggga ctgcctgtcc ggatcggcac ctgttcattc aggcgccaat tgatggcaga
    58501 gcaattttta tcaaaagccc tgccacggac cggccctgaa ataaagattg agtgcctccc
    58561 catccggggc aatgtggaca gccggctccg gaaactcggc acggacagct acgacggcat
    58621 catactggcc ctggcaggca tcaaccggat gctgagggat gaaggaaccc gtaaggaaat
    58681 gcaggttctg ctccaggaca aaaaatggat gatcctgccg cttattgaat gtgtgcccgc
    58741 tccctgccag ggggctattg tgacggaggc atgcccggac aacgtccttg cacgacaggt
    58801 ctttcaacgc atcatggatc cggtcctgca tgcggattgt tccgatgaaa aatacgctgc
    58861 aaggcaatac ggcagcggtt gcctccagcg gtttggggtc accaccctgc gggccgcttc
    58921 tgaaaaatgg atctatgcag caggcctgga tgagaaagga aggaccttta gagactggta
    58981 tggtattgcg tcccctgtat ttccattgga tcctgcgtcg atcatcactt cagatgaact
    59041 gggattcaag gcctttcgca agcccgtccc tgttcaggat ggatcgcctt caagccttgt
    59101 atattttatc gcgcaccggc atgccctgga cggctttacc aatcaacaag ccttagagca
    59161 aggaaggatt tgggcggcag ggaccggcac ctggtatgtg ctcgcccaaa aaggattttg
    59221 ggtggaaggc tgtgccgatt cttttggact ggaatcgctc accggccttt ttgactctcc
    59281 cgcagttggg atccgccggg aagaaatcct cattcttacc aatgccgaat ccacaaatcg
    59341 atggaaagcc cgcggatgga atgccctggg cacctatgaa gcccggtacg aatcgagtct
    59401 gatcgataaa tacaaactca aagaggcccg gttgatcttc tggaattgtt tcgcccattt
    59461 tgacgaggtc aaagacctgg caggaaagga tcttatccac gcctgcttac ccggccgcac
    59521 ggcagaaggg ttgaaaaaat ccggtattca tcccattctg tttccaagct tagattcatt
    59581 taatcaatgg aagaaaaatt atattcaatc catcatcgcc gcctgagatt gggtaaacgg
    59641 atccgggaat tggtaaatcc attcaggatc agccaccgga attttattta ccccctcttt
    59701 gtggatgaat ccctcaccgc ccggcgggag attgaacata tgaacggaat ctgttccgaa
    59761 acccgggaga ccgtgttcga agccatctcc tccaccctgg cttccggaat aaataaattc
    59821 ctcctgttcc cgattcccgc aaaaaaacga aaagaccatt ttgatttttc ctttgccgcg
    59881 gacctggtgg ctgaattaaa acaatcgttt ggggacggga tctggctggc aacggatatt
    59941 tgcctttgca gttataccga tcatggccat tgcgggatac tcaatgaaaa aggggaccag
    60001 gtcctgaatc atgaatccgt aaaggtcctg ggtgaatatg cctacctgtt ggcgaaagcc
    60061 ggggcagact gtgtggcccc cagcgatatg atggatggac gggtgggcca tatccggcag
    60121 cagttgaatg ccttcgcttt ggagcaggta accatcatga gctattcggc caaattcagt
    60181 tcgcaatggt atggtccgtt ccgggacgcc tgtcattccg caccggatca aagccttggc
    60241 attaaagacc ggaaatccta tcagttatcc ccgttaagta gccgggacgc cgttcaatgt
    60301 gccttacgcg atgaagccga aggggccgat atcctgatgg tgaaaccggc tgcgctctat
    60361 acggacgtca tttggtctgt gaagcaaaag acaaacctcc ccattgccgc atatcatgtg
    60421 agcggggaat atgcctcggt cgaactgatg gcagcacagg gtgtggtcga ccgggctaag
    60481 gctcacctgg aagtctgggc ggccctgcaa cgaagcggag ctgatatcat tatatcctac
    60541 gccgcaccct ttgcaaaatc ctggattgaa aattatgatt tttaattggc ctgccggatg
    60601 aatcaagcaa aatcattcta tttaccggat cgtccttcta gggttgatcg caatgtcctg
    60661 cggaagccat ggcggtaacg aagcagattg aacggaatgt attgtacccg gttttcctga
    60721 aactggacga cctgaatata ttggtggtgg gcggcggcaa tgtcgccttg gaaaagctgc
    60781 attccatcct gagtaattca cccagcgccc ggatcaccct ggtggccccg aggatcctgg
    60841 atgccgtcaa aacgctcacg gcacaaaaaa ccaattgtac attgatcaac cggccctttg
    60901 aggactccga cctggacggc agggatctgg cctttctgac taccgatcaa cctgacctgc
    60961 atcgccacat ccgggattta gcccggcaaa aaaaagtact ggtcaatacg gccgacacgc
    61021 ccgaactctg tgatttttat ctttcgagca ttgtccagaa gggccagctc aaactggcca
    61081 tatccaccaa cggcatgtcg cctacggcag ccaaacggat aaaagaaatg ctcaaatcgg
    61141 ccttaccgga cagcctggat gaaatcatcc tgaatctgaa taccatccgc aaatccttaa
    61201 aaggcgattt tgaatacaaa acccaaaccc tgaatgagat cacccggatc tgggttgaga
    61261 ataagaatga caaggcggcc cggaggtgga aaaaaatggc gagcatctcc ctcctggttt
    61321 ttgcatccat gttgatcggc tattttttat tttcagaaat caacctgcgc gaactctccg
    61381 gaagaggcgc ggactgggtg gtcagcctgg accggagctt ttatatcatg gtgctggccg
    61441 gtttcctcgc acagttggtg gacggttctc tgggaatggg atatggggtc accagcgcca
    61501 tcgtattgtt gtcttcgggc atcagcccgg ctgccatcag cgggagcatc cacaccgcgg
    61561 aaatatttgc cagcggggca tcgggttaca gccattacag gtttgggaat gtaaacatga
    61621 aattgtttaa ggcgctggtc gtcccggggg tcatcggggc catcctgggc gcactcctcc
    61681 tagtgttttt tgggaattca ggcttcggat ggttaaggcc attgctggcc acctacaccc
    61741 tgtttttggg gatcaaattt tttatcaacg cctttcagcc tcctcgccca aaaaggaaat
    61801 tcaagtttta ccggaccctt gcaggttttg gaggtttcct cgactctttt gggggcgggg
    61861 gctggggtcc cctggttacc agcaccctgg tgagtcgtgg ccggaccgtc aagtacgtca
    61921 tcggctcggt aagcctcacg gagttttttg taaccatggc cagcgcattg actttctttt
    61981 ccctgcttgg tctcagccat tggaagacca tccttggttt gatcatcggc ggtctgcttg
    62041 ctgctcccct ggccgccctg ttgattggca agattccccg gaagaccgca tttattctcc
    62101 ttggaaccat agtcgtcatc tggagcatac gcatcctttg gggagcgttt taaggttcaa
    62161 aaggaaagag aagttatccg gacagacagc ttctccattc atccgttcgg aaacgggcgc
    62221 ctcattcgga tttcattttg gcataatcca ccataccctg gaaatcaatc accactacgg
    62281 gttcattacc cactacccag gcatcgtgtc cgctgggcag ggaggacaca tcaccggcct
    62341 tgcaatcaaa ggtcgttccg tcgtccatca ggatgcgcaa gaccccgctc acgtgatatt
    62401 ggtaatgcgg cgcttcacag cttttggttc ctgatgattc ttttaaggaa gcagaccact
    62461 tccatcc
//
LOCUS       DAOQXT010000014        62213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_507_length_62213_cov_7.646610, whole genome shotgun sequence.
ACCESSION   DAOQXT010000014 DAOQXT010000000
VERSION     DAOQXT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 62213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62213
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_507_length_62213_cov_7.646610"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1063)
                     /locus_tag="PKM27_03770"
     CDS             complement(20..1063)
                     /locus_tag="PKM27_03770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR06407.1"
                     /translation="MIKFSICIPHFNRFEYLKKSLKTLELQSYPELEIIVGDDCSSDE
                     TKLGIQDIQLTFRHKLVYHRFESNQGYDRNFRKSIELASGHYAIVIGNDDTINPDFSL
                     LKLADFIENNGFPELGFSNFLEDEIKRKFVQRAAATAILGSGYGVAMNYYSCFSFVGG
                     TIWKKESFDRFNSESQDGSIYSQIYLAVLMISRGCRLFSIKEPMVIKDLVLENQKERN
                     SYLQTIARKWTDYKPLNGGLHSVIHVLIEGLRAAGVLNQARTYLVFRRIYTLTYPYWL
                     LDYRTHGAFPAAVGLMQGMVPHRSPDIGLLNGMQTIRLWVWYSLASLAGLVTPLFIFT
                     RTKSLLYKMVKRR"
     gene            complement(1079..1744)
                     /locus_tag="PKM27_03775"
     CDS             complement(1079..1744)
                     /locus_tag="PKM27_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR06408.1"
                     /translation="MDLIKINLGCGHVRPAGWINTDSSWNANLQRSPFLRKIVVPLFK
                     HVVYKNHNLTYMNLNRTWKWGNDSVDIVYASHLYEHLTLDSARLMLRESFRCLKPGGV
                     IRLVVPDLFKLCKKYILEFENQNSKASESLLWALNLHREGQYGKLNFLKKLIFEIQGY
                     PHQHKYMYDSRSLSEKLLHAGFMDLKECRYGQSVYMESCIKEVEGNVESYLSVYLEAL
                     KPF"
     gene            complement(1756..2904)
                     /locus_tag="PKM27_03780"
     CDS             complement(1756..2904)
                     /locus_tag="PKM27_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06409.1"
                     /translation="MKKLCILHFQPVEKYPPAFNLTRLLAATHMQHVEIHLITTDAGN
                     GKQKMYIPNVNIHREAKWQSDMSRTSRLFLYLRFFICAFLRLSKVKPSALLYYETVSA
                     AAPYFYNRWLNSSCRIFIHYHEYISPGEYSSGPIFNRWLHRLEKHLYKKSYWVSHTNE
                     DRMRLFIKDLGKDAPTKTYILPNYPPASWAKTGPVTIKDGLNRIGFVYVGALSSETMY
                     FKEMANFISSRPSECYWDIYSDNYSPEIIQYLDSVVPRNIHFKGFVKYDQLPDILPKY
                     RIGLVLYKGHIPNYTYNIPNKLFEYHVCGLDVWFPKQMVTSDTLSTKGTYPKISSVDF
                     EKLERISLSAMVDRSGLEYRKNEFICENTYEPLIRELLNEKNALRITP"
     gene            complement(3980..4576)
                     /locus_tag="PKM27_03785"
     CDS             complement(3980..4576)
                     /locus_tag="PKM27_03785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNR06410.1"
                     /translation="MLIKKSIEFYKKHKGKGLTRKILFYLNKKLTFPIGRVLRSRLFF
                     NNSTVIIGRNVIIHGLSNRVFCGMNVNIYDNVIIEFGSESELVVGDNCLFSYGVVIAV
                     NSKIKIGNDVQIGEYTSIRDTTHRYDRKDVPMKIAGDTSDPISIGNNVWIGRGCLILP
                     GTIIEDGVVIGANSVVKGALRKDGIYAGNPCQFIKLRF"
     gene            complement(4583..5470)
                     /locus_tag="PKM27_03790"
     CDS             complement(4583..5470)
                     /locus_tag="PKM27_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR06411.1"
                     /translation="MVSYNSARFIKDSIQSILNQSYVDFELIICDDNSTDNTWEIISE
                     YNDTRILKIKNEVNIGENANRNNAISLSKGQWLIFIDADDILYDYGLEIFVRHVTNNE
                     DCGMIIARPWDERIRYPFKIYPLQFYQFEYLDNSIIGRNFTKVLFKKSAIIETGCFDN
                     MQIKMGDAYIQYKIGMLYPSIIIQDGLSWWRRRKGQASEKLIQNTDLFIIDSYKYLVP
                     LLANKNNPLNNEQKEIALNNLYGSYSRFVLKLLITFKFSKVISLLKIHPIPIKYFKSI
                     LRPQKRDYFRNYSGENPLV"
     gene            complement(5524..6444)
                     /locus_tag="PKM27_03795"
     CDS             complement(5524..6444)
                     /locus_tag="PKM27_03795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR06412.1"
                     /translation="MMNNIKSKLAGLLNRFPYIKTLYKFRSDYYKNSCYEPGHYYSPI
                     VNMDDIKRNEIQIWKNKSVEVIPGINLDSEEQLLLLNEFGKYYQGIPFSAIKQKNRRY
                     YFENDLFSYTDGIMLYSFIRHFKPRRLIEAGSGFTSALFLDVNSIYFEKSIKLSFIEP
                     YPERLYSLISEEDKKNTEIIVDGIQSVDESYFEQLKENDILFIDGSHISKTGSDVNFV
                     LFEILPKLHSGVLIHFHDIFYPFEYPKEWVYGGFNWNEIYLIHAFLMNNAEYKIKLFA
                     HYLHTHHPEAFREMPLCYKNSGGSLWLQKK"
     gene            complement(6441..7571)
                     /locus_tag="PKM27_03800"
     CDS             complement(6441..7571)
                     /locus_tag="PKM27_03800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR06413.1"
                     /translation="MKKILLVSHSQKLGGAERCLYELASGLMKQYEVIITLPSKGENF
                     QMFNELGCKLIFVSYPWWLHFSYEKISYYKIIRKTFSQLYAILKFCLYILKIEPAFII
                     SNTSTVAAAALASKILGKKHYWFLHELLDEDHGLKFDYGWKFSTIVISHTSKKIFVNS
                     KFVKSKFGTFIKEEKLQVINMNVPKPSITLAQENNLDPRVINLYVIGQIQSGKGQLMA
                     IKAHQILLKKNYISRLTIIGSVSDKDYFELITNYIDSNSITAVEFKSFAIHPFQHIAS
                     NAIGLMCSEFEAFGRVTIEFMKVGIPVVGCNSGETKNIIQDGYTGLLFMKNDASDLSA
                     KILALVTNSDMKNEIVKRAKLFAEETFNATDYFQQIHRVLNA"
     gene            complement(7568..8443)
                     /locus_tag="PKM27_03805"
     CDS             complement(7568..8443)
                     /locus_tag="PKM27_03805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNR06414.1"
                     /translation="MITIGLLAYNKPQFISRAINSVLNQNFKSFSLIISDDSSNNDVE
                     NIVNSKYSDDSRITYIRRPRIGMTNNFVATMLTCTTKYFIWLCDDDYLSEDYLEKTIT
                     FLEENNEYALVTGTTRFFDGNGILDRHEFLNLEDDNPSKRVINYFRKVNSNIILYGVM
                     KTAEVKHFIYPDIFGADLFWSSQVVCSGKVKILGDTFFYYNLEGISSDTQKLAEYYNR
                     NQKKKNPYLIVRNVAFNMIIKRKGAFRKLKFIQSKLLSFRVWLILRDRFCLTKLESTI
                     RQNLKLRSRWKFIFR"
     gene            complement(8443..9375)
                     /locus_tag="PKM27_03810"
     CDS             complement(8443..9375)
                     /locus_tag="PKM27_03810"
                     /inference="COORDINATES: protein motif:HMM:NF016602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06415.1"
                     /translation="MIIDAFLMSSETDLLDLRLNYYKDYFDFFLILESSFTFSGQTRN
                     LVFPNVKHKYLKFEDKIIYVPINEFPKFSDSWGIEFYVRDQIRKISINKFSDEDLIVI
                     SDVDEIINIEKINLKATKVPTLIKLDTYYHFLNIKASEVINVNLITPLKFIKSIDIGN
                     RYTYYQFVVQQIENNGWHLTYMFEGNIDAYQKKIEAFSHQELNLAKYKNKENINNCLK
                     YALDIYNRKWVKYKFVNISNEFSANFLKVIRDLGLLNKWTTKNLNLLNPRYWLNLTSL
                     VMYYRNQFWFYRNSFVKKSKIYPFYRFVKQLINF"
     gene            complement(9372..10121)
                     /locus_tag="PKM27_03815"
     CDS             complement(9372..10121)
                     /locus_tag="PKM27_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNR06416.1"
                     /translation="MGKLLNKFSKFCQWFLLTSSIKTSLKLLGKSNGQEINFQFHGND
                     LFLRKNEADLAVFESVFQHKHHRSVVKLFNDYYVIVDLGSNIGLTLIDYAEEYKNAKI
                     YGVELDFDNYEIAKKNINSFRNIFLINAGIWNEDGWVHYEGKDPQSFAISDSAIGTKV
                     PSITMTSLIKKFGLKKIDYLKMDIEGAEYQIFKKDIDWLYLVECLNIEIHDTIEIKKE
                     DGISIISEILKGKGFNVFTNSNHWSSISAYK"
     gene            complement(10106..11326)
                     /locus_tag="PKM27_03820"
     CDS             complement(10106..11326)
                     /locus_tag="PKM27_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HNR06417.1"
                     /translation="MPVITAENLSKKFVIAHESRERYTALRDVMARGVKKIFSFPGSL
                     WSGSTSRATKEEFWALKDLNFEINQGDRVGIIGRNGAGKSTLLKILSRITPPTEGKVT
                     IKGRVASLLEVGTGFHPELTGRENIFLNGAILGMSRAEIRKKFDEIVAFAEVEKFLDT
                     PVKRYSSGMYVRLAFSVAAHLEPEILIVDEVLAVGDAQFQKKCLGKMEDVSKNEGRTV
                     LFVSHQFESLRAFCNTGVLLANGILASQSSLEECIEVYQRSTNVQRNCTYTNDDGLFF
                     KKMILKEDQIQIGNSITIKIAHSLKYNPNNLIIAVNIKNEFGETITQILNVDNKVNSI
                     SKPGEFNIEIQNLNFIPGIYNIALWVGFDMINEIEKIDNVLQFEVSNQNFSDRHTPFS
                     KSLKTFHKSKWESF"
     gene            complement(11469..12290)
                     /locus_tag="PKM27_03825"
     CDS             complement(11469..12290)
                     /locus_tag="PKM27_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR06418.1"
                     /translation="MRTDEITITPDSVERHYWMDLWRYRELFYILSWRDLKVRYKQTV
                     LGLAWSVLRPVLTTLVFYIVFNNIAKLESPGAAPYILLVFAGMVPWQFFGNALSEASN
                     SLVGNSNLISKVYFPRMIIPASSVITSLVDLLISLVILVILFIYFQYLPDLKVLLLPV
                     FILMAFIAAFGTGLYLTALNVKYRDFRYIIPFIIQFGLYITPVGYSSAVIPDQYRLWY
                     SLNPMVGVIDGFRWCLLDEPFYRDGFLISLAICLFFLWIGIRHFRRTEKSFADVI"
     gene            complement(12742..12942)
                     /locus_tag="PKM27_03830"
     CDS             complement(12742..12942)
                     /locus_tag="PKM27_03830"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR06419.1"
                     /translation="MKFRVSLIPNENGGFTVYVPVLKGCISEGNSKEEALQNIKEAIE
                     LYLEEVEDESLLEESFTEEINI"
     gene            complement(13152..14216)
                     /gene="wecB"
                     /locus_tag="PKM27_03835"
     CDS             complement(13152..14216)
                     /gene="wecB"
                     /locus_tag="PKM27_03835"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNR06420.1"
                     /translation="MIATILGARPQFIKAAVVSAELKKLGIEEYIIHTGQHYDANMSE
                     VFWKELELPSPAINLNVGSASPGKQIALMLERLEAYLMPFLDKIRLVLVYGDTNSTLA
                     GALAAANLGIPVAHIEAGLRSFDKTMPEEINRVLTDHLSRIFFCSSAQGVKWLKREGI
                     DDQVYEVGDVMADALTRYLPLAEEKIRLENILPFDAGEQFILLTIHRQSNTMSKDRLA
                     SLIEAVGTLGMRVFWPVHPRSQQALKDLKLPNNIHLAGPLSYLEMLKVLNHSYKLITD
                     SGGLQKEAYWLKKPCITLRTETEWVETLENKWNRLAPDTESLRSVFGHGPEAGSWKPL
                     YTYAEPASKAIARILGEIYG"
     gene            complement(14340..15305)
                     /locus_tag="PKM27_03840"
     CDS             complement(14340..15305)
                     /locus_tag="PKM27_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06421.1"
                     /translation="MNTFALIGAAGYVAPRHLKAIKDTGNVLVAAFDKFDSVGVMDSF
                     FPGADFFVEFERFDRHLEKRKREGKKLDYISVCSPNYLHDAHIRFGLRQGADVICEKP
                     VVLNPWNIDALKEIETESGHRVNTILQLRLHPKIVELRDRILKGPPDKIYDIDLCYIT
                     ARGHWYYTSWKGDITKSGGIATNIGVHFFDVLGWIFGPVRQNTVHLHTHDRAAGFLDL
                     EKARVRWFLSIEASTLPEEIKEKNGRSYRSLTIEGEEVDFSEGFTELHTLCYRDILDG
                     KGYGLEDARPSIQTVYEIRNAAPAGLKGDYHPFAAKPLTKHPFNL"
     gene            complement(15302..16660)
                     /locus_tag="PKM27_03845"
     CDS             complement(15302..16660)
                     /locus_tag="PKM27_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNR06422.1"
                     /translation="MIQKIVIWLRSQYRLSASSGAVRDIAKLMTGSLLSQVAPILAAP
                     ILTRIYQPSDYGLVGLYISISGLIGLVATLQFNQAIVVAKNDEEAVNLITLCAQLLVG
                     GSILSFLLIWGLMFAGIVPPLPFSFNYFWLLPFSVLCTGLASVFGSYALRIRQIRLIS
                     SGNILTAMVSTFCSIGFGLWFRNLTGLFLGLWLSQFANSVYLFSGTIYKNPAFLFKKL
                     FLNQWATAKKYANFFRFSLPASFINNFTHQVPVLMLNAWSLPAAAGSFNLSRRMLGLP
                     MAAVSGPIGEIYFQRASKAYREKGGFSRIFLWTLKSLSLFSILPFALLILFAPWLFVF
                     IFGESWEEAGQFARIMGLMYFLRFTTSPLSRTMLIVDKQYITLLIDLYLFISNALIFY
                     ISLYLLGSVSKAVLFYSLNYSLLSLFSLWISYKYSKSEMEGNSDAARLPLTNNHKDAT
                     QP"
     gene            complement(16657..17688)
                     /locus_tag="PKM27_03850"
     CDS             complement(16657..17688)
                     /locus_tag="PKM27_03850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR06423.1"
                     /translation="MGISPQEGMTEVVHFQRKPRPFGNYSLESYFKSIRELAPADIRI
                     NLKISRFESRGLWRRIYNVIEAAFSQKQVNHVTGDVHFLTLLMNRKKTLLTILDCGRL
                     KELSGIRLKLFKYFWFTLPAKKAAFITVISLATRDDLLQHIHYDPARIRVIYVNIPDL
                     YRPSEKPFNEARPLILQVGTARNKNLERIIPALEGLSCRYRILGPLTEAHSALLKKHK
                     LEYEHIDRVDTDAEVFDLYKSCDIVSFASTVEGFGMPILEGNATGRVVVTGNTSSMPE
                     IAADAACLVDPWDIESIRNGFRKVIADPDYRNQLIRNGFDNVKRFNGNLIAGQYFELY
                     RELARKSLS"
     gene            complement(17661..18698)
                     /locus_tag="PKM27_03855"
     CDS             complement(17661..18698)
                     /locus_tag="PKM27_03855"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR06424.1"
                     /translation="MQSNSTIRQFNNSTTRTAIITGIGGQDGAYLAKYLISLGYRVTG
                     ITRSLTTFQDFRLKYLGILDQVRLIECREMDRASLVGILEEVRPEEVYNLAAQSSVGL
                     SFEQPYATVQFNILSVLSWLEAILETNKAVKFYQASSSEMFGNIDRNKLPLRENLMFH
                     PASPYGISKAAAHWLVVNYREARKMFAANGILFNHESALRGMNYVVKKIINHLVKIKL
                     QWTDQPLTVGNTAIRRDWGYAPYYVAAMHRILQHHSADDFLVCSGNVMSLDDLIDKAC
                     NRLGLDKERYVKTDPALYRPNELFEIYGDPSKARLELGWKYELSNDELIAKLIEDEID
                     FIHWESAHRKA"
     gene            complement(18785..20563)
                     /gene="asnB"
                     /locus_tag="PKM27_03860"
     CDS             complement(18785..20563)
                     /gene="asnB"
                     /locus_tag="PKM27_03860"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNR06425.1"
                     /translation="MCRLAGFIDPGLKLTDLIRMRDSMYRGGPDDQGTFVDEDSRVYL
                     GHNRLSIIDLGPGGHQPMSDEGGQVHLIYNGEVYNYVALRDELTALGHKFKTRSDTEV
                     ILQAYLQWGNMGFSRLNGMFALAIWDKRKNSMILARDHAGIKPLYYAVRGGNLYFASE
                     IKAFRALQPDWPEDKNWKIFFLAFGHIPAPRTTLQQVQSLAKGSILEFEIGQSRIKLH
                     SFIPGHDIKEKVAPGEAALRVRSTLEQAIRRHLISDAPLGIFLSGGIDSSLLTLLAKP
                     WVKEHLHTLSVHFNESGYSEKKYQDQVVQMAGSRHHEYVVTSADFDLQLPDALRAMDQ
                     PSTDAINVYMISGYAHRAGLKSVLSGIGADELFGGYPSVKNSSWWQWLVKCPGPVLEL
                     ATLGTSDRYRKIKFATLKNSTGEYLFYRGLHTIDRIADILEADETEVRHQLDSMQTAS
                     WNGEDRLEKASWLEYHAYMHNQLLKDADYMSMWHSLEIRVPFLDKEFVELVNTMPSDI
                     RFKPGQPKFLLIESCKDILPEAIWNRPKQGFTLPFELWMKTSEQVRPQTPKEEKYFQA
                     FKQGRLSWARYWAIKMAGSWKLEARS"
     gene            complement(20591..21154)
                     /locus_tag="PKM27_03865"
     CDS             complement(20591..21154)
                     /locus_tag="PKM27_03865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNR06426.1"
                     /translation="MIHPSSYIDPGAIIGEGTRIWHFCHIMPGSVIGKECSLGQNVVV
                     MPGVRLGDRVKVQNNVSIYTGVTCEDEVFLGPSMVFTNVINPRSAIERKDEYQKTLVR
                     KGATIGANATILCGIEIGEYAFIGAGAVVTRDVPPYALVIGNPAYQAGWMSRAGHKLF
                     FDNQGLATCPETNQLYRLENDKVHPIP"
     gene            21153..21308
                     /locus_tag="PKM27_03870"
     CDS             21153..21308
                     /locus_tag="PKM27_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06427.1"
                     /translation="MVEAEDEVEAKAKVEVEAKIKIEGEVEVEVEVEVEVEVEVNGST
                     LTLTLTF"
     gene            complement(21297..21656)
                     /locus_tag="PKM27_03875"
     CDS             complement(21297..21656)
                     /locus_tag="PKM27_03875"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR06428.1"
                     /translation="MKYITFKEMLVWQKAMHVATEIFILTKSLPRAEDYGLTSQIRRA
                     ALSVSNNIAEGFGRHNNRDKAYYYIVSRDSSFETQNCLEYGLRVEYFKKEETNKICES
                     LNEVIHELNKIIKTLKG"
     gene            complement(21774..22952)
                     /gene="lhgO"
                     /locus_tag="PKM27_03880"
     CDS             complement(21774..22952)
                     /gene="lhgO"
                     /locus_tag="PKM27_03880"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HNR06429.1"
                     /translation="MTIIGGGIVGLATAYQLLQSRPDLRVRVYEKEKSVAMHQTSHNS
                     GVIHSGIYYRPGSKKALNCLRGYAMMLQFCDEHAIPYRICGKVIVATEESERPGLKKI
                     YERGIQNDMKGLELIGRDQLKEIEPHAEGIEAIWVPQAGIIDYKSVALKLKSKIEEAG
                     GRVICGSPVTTIEKNDSGWSVKTAEEETQAGQVISCGGLHSDRLARMTDPSYDLRILP
                     FRGEYYTLRPEAYHLVKTLIYPVPNPNFPFLGVHFTSLVHGGVEAGPNAVWALGREAY
                     NWKIFNWKDTWDTLSYPGFRKLAGKYWKDGIFEMYRSLSKPAFVNALQKLVPEIQSKD
                     LAPGGSGVRAQAVDRQGNLVEDFLFRENDQLLHVVNAPSPAATSSLSIGKTIADRVLG
                     "
     gene            complement(23060..24352)
                     /locus_tag="PKM27_03885"
     CDS             complement(23060..24352)
                     /locus_tag="PKM27_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNR06430.1"
                     /translation="MFESVKNKDSTIAVLGLGYVGLPLALEFARRFRVIGFDINPDRV
                     ALMQGKEDPSGEVRAADFEGRDILFTSVLDDLRDCQVYIVAVPTDIDENKVPDLKPLR
                     TASGFIGQVIKPGDYVIFESTVYPGCTEEECIPVIEEVSGLRLNQDFKAGYSPERINP
                     GDKTRPLASILKIVSGSDEEALENITALYGSIIHAGLHQAPSIKVAEAAKVIENIQRD
                     LNISLMNELSILFDLMEIDTHEVIEAAATKWNFIKFYPGLVGGHCIGVDPYYLLHKAK
                     ALGLEPQVIASGRRINDEMPAYIAKKLVQKLLAAGKNPINCRVLILGVTFKENVSDIR
                     NSKVSDLIHELLEYSLQVEAVDPNVDPGAFSREYGFELSSRETPVYDAVIVAVAHRQF
                     QSNPVQYYKDLLDPAFPLLLDLKGIIYEWREGFEYWKL"
     gene            complement(24361..25683)
                     /locus_tag="PKM27_03890"
     CDS             complement(24361..25683)
                     /locus_tag="PKM27_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06431.1"
                     /translation="MLEICLPQKFYPETKYTFEILLEEFLDLSCSFRMDAGLKDFEIR
                     GPGLKTIIIKNQFFIRYEESEGYIRKEAIPGAIMSWSDPEWSLKNLPVIYGEGILEDT
                     REAYVLHADLIASTYFMLSRWEEVVDDQKDEHDRSTASLSLAFRYQFLDRPVVNEYAD
                     LLWLLLLKAGYVGKRKHHMYTPVVTHDVDQPYQWPDWYTSLKHLGGDLLKRRDLRMFS
                     DNLGSLVRTRFFNQPDPFDQHQYMLDLAERYGLKVYFNFLISRSSKYDEALSSQDHRL
                     VSLIRSVEDAGHSIGFHPGYNRYLEPEPFNQELAELQNLVRQKIKSGRQHFMRFRVPF
                     TWRLWDQAGFEWESSLAYSGQPGFRCGTCYTFTVYDCLERKKLRLREKPLLLMDATLV
                     FYQKKPVDSGLTRLKDQCKKHGGEWVTLWHNNLVHHPLLNGFEELIYT"
     gene            complement(25779..26906)
                     /locus_tag="PKM27_03895"
     CDS             complement(25779..26906)
                     /locus_tag="PKM27_03895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR06432.1"
                     /translation="MQMVDLKSQYHRIKTEIDEAVLHVLEHAQFINGPEVREFQQALE
                     RYLDIPHVIPCANGTDALQIALMALDCKPGDEIIVPAFTYVATAEVIALLQLRPVMID
                     VDYDSFNCTPELIRQAITPKTKAIVPVHLFGQVAEMQGILDLAGKLHIPVIEDNAQAI
                     GADYVFADGKKQKAGTLGHIGCTSFFPSKNLGCYGDGGAMMCRDEALAEKLRTIANHG
                     QRKRYYHDLIGVNSRLDSIQAAVLRIKLGHLDEYCRARQNVAYAYNEGFKDIPFLCTP
                     AESPHSTHVYHQYTLKVVDGRRDALASFLTAEGIPNMIYYPVPLYDQEAFKPFRGKVD
                     FLPVTDRLCKEVISLPIHTEMKAHDQEKVIDTVRRFFIDTE"
     gene            complement(26918..28234)
                     /locus_tag="PKM27_03900"
     CDS             complement(26918..28234)
                     /locus_tag="PKM27_03900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNR06433.1"
                     /translation="MDIGVIGTGYVGLVTGTCFAEMGHQVICVDIDAQKVSRLNQGRI
                     TIYEPGLENLFERNTRQGRLTFTTELKKAVDHAEIIFLALPTPPGEDGSADLSAIIMT
                     ADQLGDLIQKYTIVVNKSTVPVGTAEKVAAVLGDKLDNNLFDVVSNPEFLREGVAVED
                     FMKPDRVVIGSSSEKALERMKHLYDPFVRQGNPILTMDLRSAEMTKYAANAYLATRIT
                     FMNEIANLCERAGADVDMVRRGIGSDNRIGKRFLFPGIGYGGSCFPKDVMALIQTARE
                     FDYEMNILNSVVRTNQDQKHILTAKLEKYFMQNLGGKQIAIWGLAFKPNTDDIREAPA
                     LTVIDDLLARGAKVRVFDPEAMDHVKDRYGDQIQYGENEYEVLDQADALVIATEWSLF
                     RAPEFDRIKSLMKTAALFDGRNLYDLDDIPPGFYYESIGRKTISTL"
     gene            complement(28269..28628)
                     /locus_tag="PKM27_03905"
     CDS             complement(28269..28628)
                     /locus_tag="PKM27_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR06434.1"
                     /translation="MKDNIIREKSYQFSLDSIRIYKILIVDKKEFILSKQFLRCATSI
                     GANIEEGLAGHSKRDFIARFQISYKEAKEVHYWIRLLKDSGYLDEEKATGLLTHAEEI
                     IRILNAILKSSKQISSP"
     gene            complement(28768..29301)
                     /locus_tag="PKM27_03910"
     CDS             complement(28768..29301)
                     /locus_tag="PKM27_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HNR06435.1"
                     /translation="MIAAACQNDLSPDTTKWFAVLTRVKAEKAVSQALTRAGIKNYIP
                     LQKVVRVYTRKKRTQMLPLIYRYVFVHIRKAEYVPVLETQHVEGFVRFNKNLIAIPDQ
                     EIEWLQRVTGEINGISLENRPFSPGEAVEVVLGNLTGLRGKLIRSLGKNELLIELDRI
                     GFGLRIQIDPAHLRSLR"
     gene            complement(29303..29881)
                     /locus_tag="PKM27_03915"
     CDS             complement(29303..29881)
                     /locus_tag="PKM27_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNR06436.1"
                     /translation="MIEDLISSKTRIKLLLKFFLNSNTTSYLRSLESEFGESTNAIRL
                     ELNKMEDAGMLNSFQEGNKKYFKANPLYPLYQEIHSILLKYIGIDQILDNILKRLGLL
                     ERAFLTGTFAQGRDSGVIDLILVGQLDKSYLIQLVEKSEKLIKRKIRYVIYDNEEELR
                     IGWSSFHPEPYLIWSAPYLEPHPVNPQNELNP"
     gene            complement(29921..31018)
                     /locus_tag="PKM27_03920"
     CDS             complement(29921..31018)
                     /locus_tag="PKM27_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06437.1"
                     /translation="MPEQININDPKIPGAYYDQDLSLKDIIVKGKGFVLLLWKGRYLI
                     LVLSLLTGTLMYLKARFQPVTFNATLTFALNEGENLGYSSISGLLGQFGLGGSGTGKV
                     SLDKIVELSRSMRIVNAALFDKAAVGGTEDFLANHIIRIYALDKKWAEADEAYRDFRF
                     RSDSLAGFSLLENAALKRVYGFTVGGEKSGLLSCTYKPESTIFSLSSQTENEELSLAL
                     TDRIFYHLSRFYIQQSSDKQKQTFDLVKAKADSIYERWTSSEYAISNMDESEGALWAP
                     TDRTRKTIQSKQSNMYAIAYGEALKNLEIADYALKNSTPFIREIDRPFPPLDAQAPNR
                     LRQLLMGLILGGVLASAWILGKNLVHEAIKS"
     gene            complement(31011..34688)
                     /gene="metH"
                     /locus_tag="PKM27_03925"
     CDS             complement(31011..34688)
                     /gene="metH"
                     /locus_tag="PKM27_03925"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNR06438.1"
                     /translation="MSDSIHEIIKQRILVLDGAMGTMIQKHALDEKGYRGERFKDYAY
                     DVKGNNELLSLTQPKIIRDIHKAYLEAGSDIIETNTFNANAVSLADYHMEGLAYELNL
                     CSAQLAKNAVEEYLASHPYRPCFVAGAIGPTNKTASLSPDVNNPGFRSIYFDELVTIY
                     YEQIRGLVDGGVDLLLVETVFDTLNCKAALFAVQQYLEDTGKLLPLMVSGTITDASGR
                     TLSGQTAEAFWISVKHANLFSVGLNCALGAKELRPHIEALSKVADCYLSAYPNAGLPN
                     ELGGYDQTPEEMESYMRDFADNGFVNIVGGCCGTTPDHIRVMARSVKGLKPRNIPKVP
                     PYTELSGLEPLIFHPDLNFVNIGERTNVTGSKAFARLIKEDKYAEALSVARQQVEGGA
                     QVIDVNMDEGLLDSEAAMKKFLLLIAAEPEIARLPIMVDSSKWKVIETGLKCLQGKGI
                     VNSISMKEGEAEFIRQAKLVHRYGAAAVVMAFDEQGQADTVERKVSICKRAYDILTKQ
                     VGFKPQDIIFDPNIFAIATGIEEHNEYAINFIEATRQIKKLCPGSLVSGGVSNLSFSF
                     RGNDHVREAMHSAFLYHAIQAGMDMGIVNAGMIEVYEEIPKDLLKLVEDVLFNRNEKA
                     TEALIQYADRIKGGGKVLQKDESWRKEPVRQRLIHSLVKGIDEYIETDTEEIRKEYPM
                     ALSVIEGPLMEGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLTPFIEAQKQSAGDS
                     SKGKILMATVKGDVHDIGKNIVGVVLGCNNYTIVDLGVMVPCDKILETALAEKVDIIG
                     LSGLITPSLDEMVYVAKEMQRRGMNLPLLIGGATTSKLHTAIKIEPQYNLAPVIHVLD
                     ASRSVAVVSALLSKDEVGRQVFIEKTRDEYTQVRKSRSERETTKTYVSLETARKQKLG
                     IDWDRYEPPKPKWLGTKVLLDYSLEELSDFIDWTPFFSSWQLNGKYPALFEDPLVGVE
                     AKKLYLDAQSLLRRIIDEKWIRAEGIVGIFKANSLETDDIEIRNEAGEVVAVAHHLRQ
                     QREKSAGIPYLSLADFIAPKSSGKTDYIGGFAVTAGIGLDEKVRELQAGHDDYHAILL
                     KSLGDRLAEAFAECMHYRMRTEWWAYSDEKLTNEELIAEKYQGIRPAPGYPACPDHTE
                     KQTLFDLLGVESKIGMNLTESMAMHPASSVSGWYFSHPQSKYFGLGNIGRDQVEEYAL
                     RKNQDISLTEKWLSPLLNYDA"
     gene            complement(34971..35135)
                     /locus_tag="PKM27_03930"
     CDS             complement(34971..35135)
                     /locus_tag="PKM27_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06439.1"
                     /translation="MKIATSLNVKLSINTFVQVNSLTRVKALNFRLRYNTKDGNDFTW
                     STTKALTTLA"
     gene            complement(35210..35830)
                     /locus_tag="PKM27_03935"
     CDS             complement(35210..35830)
                     /locus_tag="PKM27_03935"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNR06440.1"
                     /translation="MKHRIWLAISFIRVISWGQPMEMSNPVPTNDKSGKAATLTSISR
                     LRNTIDFDGQVDPLEWGSDTLDFVSHWPSYSNKPNTRTRFSMAYDEKYLYFSAICYDD
                     PGQIQGSYFERDKCGLSMDQIAILLDTYKDQENGLLFVVSPTGFRIDVSVSNDGQGEL
                     SVDLSWNSYWEAKVTRNGEGWMMEARIPFSSLRFQLTVPSASFRNT"
     gene            complement(35861..37573)
                     /locus_tag="PKM27_03940"
     CDS             complement(35861..37573)
                     /locus_tag="PKM27_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06441.1"
                     /translation="MKNIFYGICLCILFHCKSKDTPVPSGGMVAPVSMCGAQPTDAAW
                     YSTDQVAPLFEGLGDLHYPITTSHAQVQQYFDQGLKLSYAFNHAEAARSFYYATKLDS
                     ACAMCHWGFAYVLGPNYNAGMEPDNYQRAYAAVQQAIKYSGPASPKEKAMIQALATRY
                     VPDPVEDRRALDSAYSAAMKKLHETYPDDSEIAALYVESVMDLHPWDLWDKKGNPKPW
                     TPEIVAIIERLLKQYPNHPGFHHFYIHAVEASFNPGRGLESARKFDRGMVPGAGHLVH
                     MPSHIYIRTGDYHEGSLANLNAVKIDSHYVSSCHAQGVYPLAYYPHNYHFLAATATME
                     GKKDWALMAAYKMRDHVNKDLMPDPAWATLQHYYTIPYFVQVKFGEWDSILIQPEPSM
                     LPYVQAIRHYARGFARLGKGELSQAKGELSTLDSLARDSSLQELTIWGINRISDIVAI
                     ASKVLQAEILAKEKSFDASITLLKEAVSIEDALNYNEPPDWFFSVRHHLGAVLVESGH
                     YDEAIETYKADLAWLPRNGWAQHGLKKAYQLKKDPKALAAIEKDLQQSWQYAEVKLDG
                     SRMF"
     gene            37725..39185
                     /locus_tag="PKM27_03945"
     CDS             37725..39185
                     /locus_tag="PKM27_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNR06442.1"
                     /translation="MQFPILIILFVFHAINSLDNHKPPSPFGALPSARQLAWHETEVY
                     GLIHFTPTTFENKEWGFGDADPSLFNPRDFDAGQIISAAKAGGLKGLILVAKHHDGFC
                     LWPTKTTEYNISKSPWRNGKGDMVREFEQACRKAGLKFGVYCSPWDRNNEYYGQPEYL
                     DIYRAQLRELYTEYGPLFMSWHDGANGGDGYYGGKREIRKIDNTVYYDWENTWNNITR
                     KLQPMAAIFSDIGPDVRWVGNEKGIAAEKHWATFNPEAETGSIAVPGNVNTRMSNEGT
                     HRGKYWIPAECDVPLRPGWFYHPEQDGKEKSTDQVFDLYLKSVGRGACLDLGLAPGPN
                     GLLHENDVRILKQFGEKVRATFETNLIRGATLIPSHTRGKAYAAKNMQDGDRYTYWST
                     SDKVQKAKIEIVLAEKKELDLIQLRENIKLGQRVERADIEIMEQGGWRALVSVPTIGA
                     NRLIRLDNPIITDRLRITLEAPAPLCISEIGLYKIQ"
     gene            39334..40815
                     /locus_tag="PKM27_03950"
     CDS             39334..40815
                     /locus_tag="PKM27_03950"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNR06443.1"
                     /translation="MSLETLQDKIKGGWAGQAIGVTFGGPVEFRFNGTIIQSYQPLPW
                     YRGYVAEMMKSNEGLYDDLYLDLTWVEVLEKYGLHAPAEHFAKAFAYKGYKLWHANQA
                     ARYNILHGIAAPHSGHWMNNPHADCIDFQIEADFAGLMSPGMSQSAALICDRVGHIMN
                     YGDGWYGGVFIASMYSWAFISSNIPNIVSQALKSIPANTRYHQVIADVLAWYKKYPKD
                     WTKTWLEVQNKWSEDIGCPDGVYAPFNIDAAINSAYVVIGLLYGKGDFTQTIEITTRC
                     GQDADCNPASAGGILGTILGYQQIPAYWLQGLEGAEDLKFAQTDYSLNKAYEVSLKHA
                     IEHIRLNGGWVEEGTVRIVAQKPVPVKFEQSFENTYPVQKTYIGTVLENEYYFPFEGT
                     GIVVKGYVSNWDGLGPAYSAKAEIYLDEIKTETVDLPSNFLTRRHELFWKYGLPKGRH
                     TLRIKLLNRDKQYEVRITDILTYSNTRTQMEKVHHFGQTPKKN"
     gene            40887..41522
                     /locus_tag="PKM27_03955"
     CDS             40887..41522
                     /locus_tag="PKM27_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNR06444.1"
                     /translation="MHIPSHFKIEDPLIIDQFIQDHGFATLISQGSPYPLATHIPLEL
                     ETLPSGIQILHGHMSKSNPQWTYFAGHPFVLAVFMSPVHQYISSSWYRHANVSTWNYL
                     SVQVSGRIRLIEGEKLKEALRRLTHKYEKHSEQPLEFDQLPPSVQNQMRGVAGFEIEI
                     EKKEASFKLSQNRNEEDFANIIRELRKKGNPEADKMAELMIAAAQSKSNHV"
     gene            41515..42153
                     /locus_tag="PKM27_03960"
     CDS             41515..42153
                     /locus_tag="PKM27_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNR06445.1"
                     /translation="MSEFPRLFLYSLQLNEAHHRSFSELVGKHANNISIGLIENAADV
                     IPDSAGWVRDIRERLRNEGYQIEAIDLREWNGKPEALRNLMFSKDVIWTGGGHTYYLN
                     WILKSSGADGVIRDFVQAGRVYAGWSAGAAVAGPTTRFYDAMGDDPSAAPEQSWDGLG
                     LTEISIVPHINHPDFRGGALETETLLKTAGYIPLALEDDQVLIIQGEKKWVV"
     gene            complement(42164..43300)
                     /locus_tag="PKM27_03965"
     CDS             complement(42164..43300)
                     /locus_tag="PKM27_03965"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNR06446.1"
                     /translation="MKTIFNSSAGILILGAFAACKSGEQKETPAPMPPSTHQMITIAP
                     FGMIDSDTVSLFTFKNNNGMEVSVTNFGATITQLMVADRTGVPGDVLLGFNTLEGFRQ
                     AGNPFFGCIVGRYANRIAKASFSLNGKTYKLAANNGKNSLHGGIRGFDKALWKALSHT
                     DSSVTLGYLSVDGEEGFPGNLNVEVTYTLTWDNQLKIDYMATTDQATPVNLSNHAYFN
                     LSAGTDSTILGHELYINADQFTPVDESLIPLGNLALVKGTPMDFTEAKPIGRDISQIK
                     GGYDHNFVLNKPGTLAATAYHPGSGRLMTVHTSEPGVQFYTGNFLRGGHTDTKEGRNY
                     QKHAGFCLETQHFPDSPNQPAFPNTILQPGETFRSSTSYGFSVK"
     gene            43434..44666
                     /locus_tag="PKM27_03970"
     CDS             43434..44666
                     /locus_tag="PKM27_03970"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR06447.1"
                     /translation="MQHDPFAALRIREFRFFILGRLLFITGLRMMATVVAWWLYETTR
                     DPFAIGLIGLAEVIPAVGLALYAGHFLDTHERSAVLKKAVMLYGVVSFLMFILSTSLV
                     QAHTVQKTWIAGVYLLIFFTGAIRAFAGSSFQSLLGQLVPFALLPNAITWSSGTWLIS
                     SIIGHAAGGFLIALIAVKGALLISFLCFVVACLVLWKIAPKPLPESRRHASTWSSVKE
                     GLSFVYKTKDLMASMTLDLFAVLFGGAVAMIPAFAKDILNVGPLAFGWLNAATDIGSI
                     LTVTTLTLSPLKRKQGKILMIAVAGFGISIIVFGLSRSYLLSFIALFLSGMLDGISAI
                     VRGTIAQIKTPNELKGRVMSVNSMFVNSSNEFGQFESGLAARLMGLVPSVIFGGSMTL
                     LVVIITWFKAPSLRKLEY"
     gene            44785..45249
                     /locus_tag="PKM27_03975"
     CDS             44785..45249
                     /locus_tag="PKM27_03975"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNR06448.1"
                     /translation="MIHTEASIVIKRPIDEVFRLQTDLGRYKDWQDGLVEAAWTSPGP
                     AGVGSTYKFITANAGMRFELPGEIIEWDLPRGWKWKARSGPITIEGGYRFEPTGQGTK
                     LTMFSDSELKGMMKLMPAWLYKYLGERSQKRSLNQLKSLIESGNQHIQEAST"
     gene            complement(45278..46069)
                     /locus_tag="PKM27_03980"
     CDS             complement(45278..46069)
                     /locus_tag="PKM27_03980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNR06449.1"
                     /translation="MPAAVKYLPLLILSIGAGPQILTGQTGQYQNKTYVQGAITRGHP
                     AVPEIALIFSGDEFGEGLTYVRKQLKRNKVPASFFFTGRFYRNPLFRKDLRRLIRDGH
                     YMGAHSDQHLLYCDWVRRDSLLVDKHEFSTDLDLNYKAMEEAGADVSRALYYLPPFEW
                     YNDTISAWSAEKGIQLINFSQGTLSHTDYTTLADKNYRSNETIYESVVRFEKARGLNG
                     FILLMHIGAGPARPEKFYSLLPRLLVLLKERGYVFRAVDELLSNQ"
     gene            complement(46075..46716)
                     /locus_tag="PKM27_03985"
     CDS             complement(46075..46716)
                     /locus_tag="PKM27_03985"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNR06450.1"
                     /translation="MQNKADPERASAMARYMKNRFQYFGISSTERNLVFKPYRNPLLA
                     FAKKGHLETILLQTWAFDQREMQYCGLEILWAARKVYTTGTIDLIEKLILRKSWWDTV
                     DLLAAKAAGHWFSVFPEKKAARIRRWTRHPDLWLNRSAILHQLGYKGNTDFELMKKTI
                     LPHIHSKEFFLRKAIGWALRQYSRVNPQAVLEFVKEHDALSGLSKKEALRLIK"
     gene            complement(46773..47066)
                     /locus_tag="PKM27_03990"
     CDS             complement(46773..47066)
                     /locus_tag="PKM27_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06451.1"
                     /translation="MESYMVNVHLDKLDEEFWELLPAHRKIVNDLLIEGVIEMYAISE
                     DRSSGWIVFHAENREQVADLVENFPIRDYFTYEITGLFILNNAVSLWPKVHLN"
     gene            complement(47170..50451)
                     /locus_tag="PKM27_03995"
     CDS             complement(47170..50451)
                     /locus_tag="PKM27_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06452.1"
                     /translation="MAWRLIWSIGFIFGAFWSYAQSGIETEFGKNRIQHHDDFDDWYM
                     YETQNFVNYWYGKARNVGQITLSLAEYDFRSIESILEHKLNDKIEIICYTDISDLKQS
                     NIGIDEVFISRNDRTTVLGNRVFVYFDGDHNHLRRQIREGIGEVYLNAMIYGSGLQGF
                     VQKSIQLKIPDWFREGLIAYLGEDWNTRADNQFRDLLQHSPPNRKFETLAKDHPRLMG
                     YAFWNYLTREYGRSNVSNILYLARINRNIKSSFLYILGTPFEEIAAASLSYFRKNYQE
                     DRSAFRAVNENQALTIKNKKKLPVSAVRVSPDGNRFAYAVNDRGRYKVFVQDRRSGKP
                     ARIFRGGVKNNVQETDYQIPVFAWHPNGNEMVLVTEKHDVWMLYRIDLRTGKSSQDKL
                     SPEYQRVYGIDWIDDKTLLLNASTDALSDLYYYLPLTRESRRLTQDFYDDLDASYISL
                     NGEKGVLFKSNRALDTLFTMPVDTLLPIQPFNIFFMPFDLGKNIESKKLRRLTSNIHF
                     ELDKPCLSGENQVSFLFNDHGVTQRKTMVIDADWSGSLVHSSRGDHDFITFHGADQSA
                     VGVIKNGRKVLAFTEAIRVTDTLAPGYSVYIKNRMDLKGMAPQGLSTGRITGEVTSTP
                     KTIALFQSEFPDPPGMTQAPLRPAMDSEKKPSVSSPVRDSAVFKPQRTEIPEPFQFTR
                     MVASRLKFKLDNVVTTMDNGLLFGGLNSYAGQKRGFEYPPTGFLVKAQTKDLFEDYSF
                     EGGVRIPTTFNGTEYFLIFDDNKRRIDKRYAIYRQSRYSTVEISQILSQRVRNSSVIG
                     VYQLKYPFDTYQSARLAATFRFDRSTLLATDPTTLPADPETNQRFGLRAEYVYDNTTP
                     IDINRFTGTRAKFYGELVKKMQIQIVDDWKLNLAKGFMTVLGFDARHYIGLDRRTILA
                     MRLSGATSYGSERILFFLGGVDNWLTPQYDDDTPIPGDNNYAYYALGAHLRGFKQNIR
                     NGSSHVLFNTELRIPLIQYLSRRELRSVFLRNFQVVGFVDAGTAWFGRNPFDQDSPLN
                     TTSVSNPKVSISLVYARDPLVIGYGTGLRLNMLGYQVRADYAWGIETREVLKPRFYIS
                     LGSDF"
     gene            complement(50532..52682)
                     /locus_tag="PKM27_04000"
     CDS             complement(50532..52682)
                     /locus_tag="PKM27_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06453.1"
                     /translation="MWKGKQRFYTYCCWWILFISSNLVSAQAPIDRKAVVNRHRISIT
                     RFDSLSALTVGNGAFAYTLDATGMQTFPEAYAGGIPLGTQTDWAWHSFPNKGGYTFEE
                     TLREYVFNGKKTAYAVQDHEAKRKQEATDYFRSNPHRLHLANVGLEMQHDDGRLVRVA
                     DLGQIHQQMDLWTGTVTSSFALDQRLVRVITAAHPKMDAVSFRLEGPLLESNQIGIRI
                     RIPSPTGLWKDTGINWTYDSTQQSRLARHKGGAEIYASIDRSHYLIQMQWNAAADIYE
                     VQPQYFVIKPGTKGPFELTVLFSEQSSKRKVPDFEKTRLASAQAWKKFWMSGGALDLS
                     GSLDQRADELERRIVLSQYLTRVQGVSPTPPQETGLTYNSWFGRPHMEMHWWHAVHFA
                     LWGRPELMKKSLDWYFKALPNAEKLALRQGYKGVRWQKMTDPWGNETPSSIGSFLVWQ
                     QAHVIYMSELLYKIQPSRKKLNKYKELVFATADFMADFLAWDSSSHTYNLGPGLIPAQ
                     ESHDPLTTVNPTFELAYWHWALSVAQRWRKRLGLPEHPEWKDKIERLAPLPHREGVYL
                     AARSAPDSYTNPQYLRDHPSVLGASAGIPPSNGLDTSIMNHTLNTILEKWNWQTTWGW
                     DYPMMAMAACRLNRPEAAVEALLMPVQKNTYLINGHNYQDTRLTLYLPGNGGLLAAIA
                     MMCAGTESDPGGKAPGFPDNGKWKVRYEGMVKIF"
     gene            52866..53162
                     /locus_tag="PKM27_04005"
     CDS             52866..53162
                     /locus_tag="PKM27_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06454.1"
                     /translation="MNKLRYILIFTLVLALSPCRDLMILYLPNNIVLNLQPLIEEENH
                     SDTQEKSGKEKQDKYFGLYMASIRESDSRASMLIVDQKIAFENHCDSLTQPPNA"
     gene            53248..53880
                     /locus_tag="PKM27_04010"
     CDS             53248..53880
                     /locus_tag="PKM27_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNR06455.1"
                     /translation="MRTHKDILRTEMTPPQALEILKEGNLRFLNNIRINRDLLKLVDE
                     HTESQAPFAAILGCSDSRVPVEIVFDQGLGDLFVVRLAGNIASINAIGSLEFSCKLLG
                     SKIIVVLGHTSCGAVKAACDKTYLGNLETLVKSIEPAVHDESVAHLKRDSNDKDFLKK
                     VTILNIRHQINTIYHESEILREMVEKGQIILVGALYDVNSGEVQFLDPMD"
     gene            53952..55622
                     /locus_tag="PKM27_04015"
     CDS             53952..55622
                     /locus_tag="PKM27_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNR06456.1"
                     /translation="MNRISIKLSKGDLFGGLTAGIVALPLALAFGVQSGMGATAGLYG
                     AVFLGFFAALLGGTPQQISGPTGPMTVVTAATVAALIAKAGDFTTAFPAIIATFVLAG
                     LMQIVVGTLRLGSYIRYIPYPVISGFMSGIGVIIILFQFFPALGLNSPATTLEVFGAI
                     PGALAQVNIWALIFTLLTIGIIYLFPKLTRSVPSTLVALLAVTGLSLVLGHEIPVIGN
                     IPQGFPPIHLDKIFSVDPSLFMVILEAAATLAALGAVDSLLTSVIADNVTKTKHSSNK
                     ELIGQGVGNALSGLFGGIPGAGATMRTLVNVRSGGKTRLSGMIHALVLLFIALGLGTY
                     AALIPKSVLAGILITVGISILDYRALRQIRQTPLTDALVMVIVLVITVLFDLLIAVGV
                     GILLASILFMKKMGDLMEANSYILSLEKVKAEEKKINESIPESLKDSVFVKQMDGPLF
                     FGSATGFQDLLKKMPDTHFVIIRMDKVPYIDQTGFYALEDSILALEQRNIEVLLCGIN
                     EQPLQRLKAVRLIPGLISKEHVFPDFSACLEWLSVNPVSDNRHFTVPA"
     gene            55724..57343
                     /locus_tag="PKM27_04020"
     CDS             55724..57343
                     /locus_tag="PKM27_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNR06457.1"
                     /translation="MNLSNIKENLKSDLLSGFVVFLVALPLCLGIALASGAPLVAGLI
                     AGIVGGLVIGPISKSQLSVSGPAAGLTAICLAGINSLGSYELFLLAVLLGGGIQVLLA
                     FVRAGSIAYYFPSNVIKGMLTGIGIIIILKQLPYMLGYRTDAGSATASSSSFDLFDYI
                     HPGAALIGLISLAILFAWDKYKPVKMLPGALLAVVSAVLINQVFILTGAGSMVAGSGF
                     LVNLPTFDNFSSLTSQLSFPDFGGITNKEVWILALTLGIVASLESLLTIEATDRLDPN
                     KRVTPTNRELLAQGMGNITSGLIGGLPLTTVIVRSSANINAGAKSKMSTIVHGFLLLI
                     GALFFARYLNHIPLAALAAILVATGYKLSHPVVIKKMFDKSKYQWLPFVVTVAAVVLT
                     DLLTGVMIGMAFSVLAILIGNIKHAYFFKADEYKSGKLITLVLAEEVSFLNKAAIRLT
                     LSKVPKGSRVVIDASHSTYIDHDVLEIIREFQHDTAPTQGISLILKGFKEEYEIKNND
                     HVYIGEIPEPLRKLEAKKEFITQRETLNLLT"
     gene            57472..58146
                     /locus_tag="PKM27_04025"
     CDS             57472..58146
                     /locus_tag="PKM27_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNR06458.1"
                     /translation="MRIHTYENKDTLTPENALTYLKEGNLRFVNNIRVNRDLLQLVDQ
                     YSTGQFPFAAILGCSDSRVPVELIFDQGLGDLFVVRLAGNIASTNAIGSLEFSCKVLG
                     SKIIVVLGHTECGAVKSACDSVELGNVTSVLKHIEPAIALEKEIQNNRNSKNKEFVNR
                     ILHHNLDYQIETILDQSDILREMFFQHQIAIVGALYDIKTGLVSFYDNAAQKYRERAL
                     MRSAMG"
     gene            complement(58231..59373)
                     /locus_tag="PKM27_04030"
     CDS             complement(58231..59373)
                     /locus_tag="PKM27_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNR06459.1"
                     /translation="MFRREFLQNSALAAASVQLPGQGQFLDFLKPKNKLPAWRGFNLL
                     DFFNPNPSSSRPQTKEEHFKWMQDWGFDFVRIPMAYPAYLDIDRSRNISPEETYQIST
                     QATDRIEQLVHLAHKYGMHVSLNLHRAPGFCVNAGFHEPFNLWKDKAAVEAFNFHWNF
                     WAKKFKDVSRKKISFDLLNEPCVREDMNDQHSARSAVPGLLYREIAVAASEAIWKENK
                     RHLIIADGNNVGNLVVPELADLKIAQSCRGYFPGMISHYKASWSSNNRENMPELLWPG
                     QVGDKYFSRAMLEEYYKPWIDLKNQGVGVHCGECGCWIKTPHPVFLAWFGDVLDILKQ
                     NQIGFALWEFSGDFGILNSRREDVQYEEWYGNKLDRKLLQLLMKQA"
     gene            59461..60324
                     /locus_tag="PKM27_04035"
     CDS             59461..60324
                     /locus_tag="PKM27_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR06460.1"
                     /translation="MKSMLLGFIATQLSLASIFAQSGKVYDNLSLPSKILNMERKYAI
                     YLPPDYESSSRSYPVLYLLHGGGDDQTGWVQFGEVLHITDKAIAEGKATPMIIIMPDA
                     NTTRRGYSNNASGTWLYEDFFFKELMPFVEKKYRIKGEKRYRAVAGLSMGGDGSFTYA
                     LHHPELFSSACPLSAGTGPLTLDVAKTNVRRFDSTATDAQVEAFFNRQSVPLLVNNMP
                     DSLKRAVRWYIDCGDDDFLYEGNSLVHIAMRKKEIPHEFRIRNGGHTWTYWRQALPEV
                     LEFVSQAFHQY"
     gene            60426..60836
                     /locus_tag="PKM27_04040"
     CDS             60426..60836
                     /locus_tag="PKM27_04040"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNR06461.1"
                     /translation="MITSTINYEGELHTTAIHLRSGEAIVTDAPVDNMGKGAAFSPTD
                     LLATSLACCALTTMGIVANKNGINVDGASAEITKHMASDPRRVSKIDLLIKMPSDKTY
                     TPEQKTLMEYTAIHCPVAKSLHSDLLQNVLFQWG"
     gene            60827..62086
                     /locus_tag="PKM27_04045"
     CDS             60827..62086
                     /locus_tag="PKM27_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNR06462.1"
                     /translation="MGVNKIRLHFPKPVIQGKIHLDGSKSISNRVLIMLALAEDKPAG
                     LEHLSTSRDSQLMQNLLGSREEVLDTGAAGTTYRFLTAYLATRGRKVILTGSERMKQR
                     PIGELVSTLNAMGAGIEYVEKEGFPPLRFPGTPLKQIARRISIRSTISSQYISALLMI
                     APCLPLGLELQLVGEVISRPYIEMTLAMMDHFGIRSAWENDTITIEPQPYVSKPLFVE
                     GDWSAASYYYSLAAIAEEAELDLYGISNHSLQGDAAIAEIGKLFNIQTTWHPDHVNLK
                     KLPGPLPKSFEWDFIKCPDLAQTVIALCAATGVHGVFTGLQTLRIKETDRIAAMKTEL
                     KKLQVPFYRLPAKMSKASKIEYHMVEGKAVFDHTPVFATYEDHRMAMSLAPLSLIHPI
                     EIEEPEVVAKSYPGFWKDLQSLGCEIY"
BASE COUNT        15845 a        15128 c        14458 g        16782 t
ORIGIN      
        1 atcattctca tcatgctttc tatcttcttt tgaccatttt atacaaaaga cttttggtgc
       61 gggtaaaaat aaacaatgga gtcactagtc cagccaggct ggccaggctg taccataccc
      121 aaagccgaat ggtttgcatg ccattcaaca acccaatatc cgggctccgg tgaggcacca
      181 taccttgcat taggcctact gcagcaggaa aggctccatg agtcctgtaa tccagtaacc
      241 aatagggata ggtcaacgta tatattctcc tgaatacaag gtaagtcctt gcttggttca
      301 ggacgcccgc cgctcttaat ccttctatta aaacgtgaat caccgaatgt aaaccgccgt
      361 ttaacggctt ataatcggtc catttcctgg caatagtctg caggtacgag ttcctctcct
      421 tttgattctc cagcaccagg tctttaatga ccatcggttc ttttattgaa aacaaacggc
      481 atcctcggga aatcatcaaa acggccagat atatctgtga ataaatactt ccgtcctgac
      541 tttccgaatt aaaccggtca aaactctctt ttttccaaat cgttccgccc acaaaactga
      601 aacaggaata ataattcatg gccacaccat aaccgcttcc gagtatggcg gttgcggctg
      661 ccctttgcac aaattttcgt ttaatttcat cttccaggaa attggaaaat cccaattcgg
      721 gaaaaccgtt attttctata aaatccgcca atttaagaag agaaaagtct ggatttatgg
      781 tatcatcatt accgatcact atcgcatagt gtccgctggc aagttctatt gattttctaa
      841 agttgcgatc atatccctgg ttggattcga aacggtgata taccagttta tgcctgaagg
      901 ttaattgaat atcctgaata cccagtttgg tctcatcaga cgaacaatca tcgcctacaa
      961 tgatttcaag ttcagggtat gattggagtt caagggtttt cagggatttt tttaggtatt
     1021 caaacctatt gaaatgagga atgcaaatag aaaatttaat cattcttcat ttcctatttc
     1081 aaaatggctt aagcgcctcc aggtaaacag acaaatagga ttccacattc ccttccacct
     1141 ctttaataca actctccatg tatacactct ggccatacct gcattctttt aaatccataa
     1201 aaccggcatg aagcagcttc tctgataaac tcctgctgtc gtacatatat ttatgctgat
     1261 gggggtatcc ttgaatttcg aagatcagtt ttttcaaaaa attcaattta ccatattgcc
     1321 cttccctgtg caaattcaat gcccacaata atgattctga ggcctttgag ttttgatttt
     1381 caaattctaa aatgtatttt ttgcagagtt taaataaatc cgggacaacc aatctgatca
     1441 cccctccagg ctttaagcaa cgaaagcttt cccttaacat taaccgggcg gaatcaaggg
     1501 ttaaatgttc atacaagtga gaggcatata caatatccac tgaatcattt ccccatttcc
     1561 acgttctgtt aagattcata taggtaagat tatggttctt atagactaca tgcttaaata
     1621 gaggaaccac aatttttctc aaaaagggac ttctttgaag attggcattc caggaggaat
     1681 cagtattaat ccaccccgca ggtcttacat gaccacatcc caggtttatt ttgatcaggt
     1741 ccatggtacc gtcttctatg gggttatgcg taatgcattc ttttcattca atagctcccg
     1801 aatcagaggt tcataggtat tttcgcaaat aaactcgttc ttccggtatt ctaagccgga
     1861 gcggtccacc attgcagaca aggatatcct ttccagtttc tcaaaatcca cagaactgat
     1921 tttcggataa gtccccttgg tagataatgt atccgaggta accatctgct tgggaaacca
     1981 tacgtcaaga ccacatacat gatattcaaa aagtttattg ggaatattat atgtgtaatt
     2041 gggtatatgc cccttataca gcactaaacc gattctatac tttggaagaa tgtcaggaag
     2101 ttgatcatat ttgacaaaac ccttaaaatg aatattccgg ggaacaacag aatccagata
     2161 ttgaatgatc tcgggggagt aattatcgct ataaatatcc caataacact cagaaggtct
     2221 cgaagaaata aagttagcca tctccttgaa atacatggtt tcagaactga gcgctccaac
     2281 atacacaaat ccaatcctgt ttaacccatc cttgatggtc acagggcctg tcttcgccca
     2341 cgacgcaggc gggtaattgg gcagtatata agtttttgtt ggcgcatcct ttcccaaatc
     2401 ttttataaaa agtcgcatcc ggtcttcatt ggtatgcgaa acccaatatg atttcttata
     2461 taaatgtttt tccaaccggt gtagccaccg gttaaaaatc gggcccgaag aatattcccc
     2521 aggactgatg tattcatgat agtgaataaa tatcctgcag gaggaattca gccatctatt
     2581 ataaaaataa ggagcagccg ctgataccgt ttcataatat aacaaagccg aaggtttcac
     2641 ctttgacaac cgaagaaagg cacaaatgaa aaaccttaga tataagaaca atcgtgaagt
     2701 cctgctcatg tcactttgcc atttcgcctc acggtgaata ttaacatttg gaatatacat
     2761 tttttgtttt ccgttcccag catcagtagt gatcaaatga atttcaacat gttgcatatg
     2821 ggttgcagca agtagtcttg tgagattaaa agcgggtggg tatttctcca ccggctgaaa
     2881 atggaggata caaagtttct tcaaatccga aattcgttga tatcgcttaa gaacttcctt
     2941 gttctgctaa aataatcata tcttttgctc aaggagagtc ttcgggctct gcgacccaga
     3001 tctaaagcgg cctggagtgt catattcaaa atgacattta cctgtttgag gcagatttcg
     3061 gtatcggtga atagagtgac caagttgtcg ttgccgaaat actctctgtg atcaatccgg
     3121 ataaagacca atacctccct ccgccggaat ttcgaaggaa cacatattgt gcgatgttgg
     3181 attgtatggg cgcattaaac tcaattgaac cctatatcta tagaatgctt tccagaactc
     3241 ctctccatac actggaggat gcacagtgat tacttgatgg tgagtatggc ggtctcagtg
     3301 gttaccataa acatgcaaag gtattgaatc ggcgactttt tccagaaaat gcaccagata
     3361 cttgtcagga ttaccaatga agcagggttt gagcacttcc tcctgtttgg tacatcgctc
     3421 atacgaggtt gatgataatt caaagccaaa cggaaatata aaacaaggca gtttaaactg
     3481 gtacaagatg gttttctgaa caccgcggtc ataagtataa ttgatatcat acaggctgat
     3541 cgaccgggtc acatttatat ttccacaacc cctgctactg aaataaaaag tattgtctgg
     3601 attgtaattc actagtataa ttccctgctt tattagttgt ctcaaagtag aaggcaacaa
     3661 ttccattctt ttaaataccc agatcagatc tggcttatcc atttcgacta gtttgtatat
     3721 atcatcatta atggcgtaga tattttttga aagtccagat ctgtattgga gtttattaaa
     3781 aagactttca ttgaagtaat aataaaatat atcctcggct gtgaataatt ctgtttcgat
     3841 cccaaaacta ttaagatgtt ttttgtagat cctctctcca atgaccatac ttgaatggtt
     3901 cctataatta atatttttat aattttcact agaaataatt gattgttatt gaattgaact
     3961 taaactcatt atttaacaac taaaatctca atttaataaa ttgacaaggg ttgcctgcat
     4021 aaataccatc ttttcttaaa gcacccttaa ccacgctatt agcaccaatt actactccgt
     4081 cttcaataat agtacccggc aatattaagc agcctctccc tatccacaca ttattaccta
     4141 tgctgatagg atcagaagta tcccctgcaa tcttcattgg aacatcttta cggtcgtaac
     4201 gatgagtggt gtcacgaata gatgtatact cgccaatctg aacgtcattt ccaattttta
     4261 ttttagaatt tactgctatc actactccat aagagaataa acaattatcc ccaaccacta
     4321 attcactttc tgatccaaac tcaataataa cattgtcata aatatttaca ttcatcccac
     4381 aaaaaaccct atttgaaagt ccatgaatta taacatttct tcctattata acagttgaat
     4441 tattaaaaaa taacctacta cgtaaaactc tgccaatagg aaatgtcagc tttttattta
     4501 aataaaacag gattttcctt gtaagtcctt tacctttgtg cttcttataa aattctatcg
     4561 atttttttat taacattatt tcttatacaa gtggattttc acccgaataa tttctaaaat
     4621 aatctcgctt ttgaggcctt aatattgatt tgaaatattt tataggaata ggatgaatct
     4681 tcaaaagtga aattaccttt gaaaatttaa aggtaatcaa aagtttaagg acaaaacgag
     4741 aataacttcc ataaagatta ttgagtgcaa tttctttctg ttcattatta agaggattat
     4801 ttttgttagc taaaagaggt actaaatact tataactatc tatgataaaa aggtcagtat
     4861 tttggataag tttttcagat gcttgcccct ttctccttcg ccaccagctc aatccatctt
     4921 gaataatgat tgacggataa agcatcccaa ttttatattg aatatatgca tcccccatct
     4981 ttatttgcat attgtcaaaa catcctgttt caataatggc ggatttcttg aacagaacct
     5041 tggtgaaatt tctgccaatt atagaattat cgagatattc aaattgataa aactgtagtg
     5101 gatatatttt aaaaggataa cgtattcttt cgtcccaagg tcttgctatg atcataccgc
     5161 aatcttcatt attagtgaca tgcctaacaa atatttcaag gccataatca tacaaaatat
     5221 catcagcatc tataaaaatt aaccactgcc cctttgataa tgaaattgca ttgttccggt
     5281 ttgcattttc gcctatatta acttcatttt ttatctttag aattcttgta tcattatact
     5341 ctgaaattat ttcccaggta ttatcagtag agttatcatc acatatgatt aattcaaaat
     5401 ctacatatga ttgatttaaa atactttgta ttgaatcctt aataaaacgt gcagaattat
     5461 aagaaaccat taatacagaa attacaggta tagtcttcaa aataaattat aataatttgt
     5521 agtttatttt ttctgcagcc acaaactacc tccggaattt ttataacata aaggcatttc
     5581 tctaaatgct tctgggtgat gagtatgtaa ataatgggca aacaatttta tcttatattc
     5641 cgcattattc ataaggaaag catgaattaa atatatttca ttccaattga agcctccata
     5701 cacccattct ttagggtatt cgaatggata aaatatgtca tgaaaatgta tcaatacacc
     5761 ggaatgtaat ttaggaagaa tttcaaaaag tacgaagttg acatcactcc ctgtttttga
     5821 aatatgagag ccatcaataa acagtatatc attctccttc aattgttcaa agtatgattc
     5881 atctactgat tgaatcccgt caactataat ttccgtattt ttcttatctt cttcagatat
     5941 cagtgaatat aggcgctcag gatacggttc tataaagctt agctttattg atttctcaaa
     6001 ataaatgcta ttaacatcaa gaaatagagc agatgtaaaa ccagacccgg cttcaattaa
     6061 acgcctaggt ttaaaatgcc ttataaatga gtaaagcatt ataccgtctg tgtatgagaa
     6121 aagatcgttt tcaaaataat atcttctatt cttctgcttt atagcactaa acggaatccc
     6181 ctgataatac ttaccaaatt catttagtag caaaagttgt tcttcagaat ctaaatttat
     6241 tcccggtata acctcaactg atttattttt ccaaatttga atttcgttcc tttttatgtc
     6301 atccatattc actattggcg aataataatg gcctggttca taacaggaat ttttataata
     6361 gtcggatctg aatttataaa gcgtttttat atatgggaaa cggtttaata atcctgctaa
     6421 cttgctttta atattattca tcatgcattt aaaactctat gaatctgttg aaaataatct
     6481 gttgcattga aggtctcttc agcaaaaagt tttgctcgct tcactatttc attcttcata
     6541 tcactgttgg taacaagagc cagtatcttt gcacttagat ctgacgcatc atttttcata
     6601 aaaagtaacc cggtatatcc atcctgtata atatttttag tttcgcctga attacaacca
     6661 accaccggta tacctacttt catgaattca attgttactc ttccaaatgc ttcaaattct
     6721 gaacacatta aaccaatagc attgcttgca atatgttgaa aaggatggat tgcgaaagac
     6781 ttgaattcga ctgctgtgat agaatttgaa tcaatgtaat ttgtgatcaa ttcaaaataa
     6841 tctttatcag atactgatcc aattatagta agtcgggata tataattttt cttcagtaaa
     6901 atttgatgag cctttatagc cataagctgc ccctttccag attgtatctg gcctatcaca
     6961 tatagattta ttacccgcgg atctaaatta ttctcttgag ctaaagttat cgaaggtttt
     7021 gggacattca tatttataac ctgaagtttt tcctccttaa taaatgtccc aaatttactt
     7081 tttacaaact tggaatttac gaatatcttt ttgcttgtat gcgaaatcac tatcgttgag
     7141 aatttccatc catagtcgaa tttcagtcca tgatcttcat ctaataattc atgtagaaac
     7201 caatagtgtt tcttaccaag gatttttgag gccaatgcgg cagcagcaac agttgaagta
     7261 tttgaaatta taaacgctgg ttcaattttt aaaatgtaca gacagaactt taaaatagca
     7321 tataactggc taaatgtttt tcgaataatt ttataataac taattttttc ataagaaaag
     7381 tgtaaccacc atggataaga aacaaaaatc aatttacatc caagttcatt aaacatttgg
     7441 aaattttctc ctttggacgg caatgttatt attacctcat attgcttcat taggccagat
     7501 gctaattcat ataaacatct ttctgcccca ccaagttttt ggctatgaga aactaacaga
     7561 attttcttca tctaaaaata aacttccatc tactcctgag ttttaaattt tgtcttattg
     7621 ttgactctaa ttttgtaagg caaaatctat ctcttaaaat cagccatacc ctgaaggaaa
     7681 gtaatttaga ttgaataaat ttcaatttcc taaatgcccc tttccttttt ataatcatgt
     7741 taaaagctac atttctaaca atcaggtatg ggttcttctt tttttgattt ctattgtaat
     7801 attctgctaa tttttgtgtg tcagaggaga taccttctag attataatag aaaaaggtgt
     7861 cacctaatat ttttactttg ccagagcaga caacctgact gctccagaat aaatcagcgc
     7921 caaagatgtc gggataaatg aaatgcttta cttccgcagt tttcattacc ccatataaaa
     7981 taatattgct gttaaccttt ctgaaatagt ttattactct ttttgaagga ttatcatctt
     8041 caaggttaag aaattcatgt ctgtctaaaa taccatttcc atcaaagaac ctagtagttc
     8101 ctgttactag agcatattcg ttattttctt ctaaaaatgt tattgttttt tccaaataat
     8161 cttcagaaag ataatcatca tcgcaaagcc agatgaagta ttttgtagta caagttaaca
     8221 ttgttgcgac aaaattattt gtcattccaa tccttggtct tctgatatat gttattcggc
     8281 tgtcgtctga atatttacta ttaacaatat tttcaacatc gttgttacta ctgtcgtctg
     8341 aaataattaa actaaaactt ttaaagtttt gattgagaac tgaattaatt gcccgggaaa
     8401 taaattgtgg cttattgtat gctaataatc cgattgttat cattaaaaat taattaattg
     8461 tttaacaaaa cggtagaaag gatagatttt ggattttttc acaaatgaat tcctgtaaaa
     8521 ccaaaactga tttctatagt acattaccag agaggttagg ttgagccaat atctggggtt
     8581 taataaattt aaattttttg tagtccattt attcaataaa cctaaatctc tgataacctt
     8641 taaaaagttt gcactgaatt cattggaaat atttacaaac ttgtatttga cccattttct
     8701 attatatata tccaaagcat atttcaaaca attattgatg ttctccttat tcttgtattt
     8761 agctaaattt aattcttgat gagaaaaggc ttctattttt ttttgatatg catcaatatt
     8821 tccttcaaac atatacgtta aatgccaacc attattttct atttgttgaa caacaaattg
     8881 gtaatatgta tacctattac caatatcaat actttttata aattttaaag gcgtaattaa
     8941 attcacatta ataacttcag atgcttttat gttcaaaaaa tgatagtagg tgtctaactt
     9001 aataagagtt ggcacttttg tagccttcaa atttatcttt tcgatattta ttatttcatc
     9061 aacgtcagat ataactatca aatcctcatc ggagaattta tttatagaaa tttttcggat
     9121 ttgatctctt acataaaatt cgatacccca agaatcagaa aattttggga attcattaat
     9181 tggtacgtaa ataattttat cctcaaattt taaatattta tgtttgacat ttggaaaaac
     9241 cagattcctg gtttgtccag aaaatgtaaa agaagattct aatataagaa aaaaatcaaa
     9301 atagtctttg taataattca atcttaaatc taaaagatct gtctcgctgc tcattaaaaa
     9361 agcatcaata atcatttata tgcgcttatt gatgaccaat gattgctgtt cgtgaaaaca
     9421 ttaaatccct ttccttttaa tatttcagat attattgaaa taccatcttc tttctttatt
     9481 tcgattgtgt catggatttc aatgtttaaa cattctacaa gatagagcca gtcgatatct
     9541 ttcttaaata tttgatattc tgctccttcg atatccattt tcaaataatc tatttttttt
     9601 aatccaaact ttttgatcag ggaggtcatt gtgatggatg gaactttggt cccaatagct
     9661 gaatcactta tagcaaatga ttgtggatcc ttcccttcgt aatgaaccca gccatcttca
     9721 ttccatatac ccgcattgat taaaaaaata tttctaaaac tattaatgtt cttttttgct
     9781 atttcataat tgtcgaaatc caactcaacc ccataaattt ttgcattttt atactcctcg
     9841 gcataatcaa ttaaagttag tccaatgttt gaacctaagt ccacaataac gtaatagtcg
     9901 ttaaataatt taaccacact tcggtgatgt ttgtgctgga acacagactc aaagactgcc
     9961 agatcagctt cattctttcg taagaaaagg tcattaccat gaaattggaa attaatttct
    10021 tgaccattac ttttccccaa aagctttaat gaggttttaa tgctcgaggt taataaaaac
    10081 cattgacaaa attttgaaaa tttatttaga agctttccca tttagatttg tgaaaagttt
    10141 taagagactt tgaaaatggg gtatgtctgt ccgaaaaatt ttgatttgaa acttcaaatt
    10201 gcaaaacatt atcaattttt tcaatctcat ttatcatatc aaacccaacc catagcgcta
    10261 tattataaat ccctggaata aaatttaggt tttgaatttc tatgttaaac tcaccgggct
    10321 tactgatgga attgacttta ttatcaacat ttaatatttg agttatggtc tcgccaaatt
    10381 cattttttat gtttactgca attatcagat tattcggatt atattttaac gaatgtgcaa
    10441 ttttaattgt tatgctattg ccaatttgaa tttgatcttc ctttaaaatc atttttttga
    10501 aaaatagtcc atcatcattg gtgtatgtac agtttctttg aacatttgta ctgcgttgat
    10561 aaacctcgat acattcttcc aacgaacttt gactagccaa aattccgtta gctaataata
    10621 ctccagtatt acaaaatgct cttagagatt caaattgatg actcacgaac aacactgtcc
    10681 ttccctcatt cttactcaca tcttccatct tgcccaaaca ttttttttga aattgtgcat
    10741 caccgacggc caatacctca tcaactatca atatctccgg ttccaaatgg gccgctaccg
    10801 aaaaagccag ccgcacatac ataccgctgc tataccgttt cactggtgta tccaggaatt
    10861 tctccacttc ggcgaaagcc acgatctcgt caaacttttt ccggatctcc gcccggctca
    10921 tgccaagaat agcgccgttg aggaagatgt tttcacggcc ggtgagttcg ggatggaaac
    10981 ccgtgcccac ctcaagcagg ctggcgaccc ggcctttgat cgtcaccttg ccttcagtag
    11041 gcggggtgat ccggctgagg attttcagga gggtgctctt acccgccccg ttcctcccga
    11101 taatgccaac gcgatccccc tggttgatct caaaattcag gtctttgagg gcccagaatt
    11161 cttccttagt tgccctggag gtgcttccgg accataagct gccggggaaa gaaaaaatct
    11221 ttttcactcc ccgggccatc acatcccgca gggcggtata acgctccctg gattcgtggg
    11281 caatcacgaa ctttttagag agattttcgg cggttatgac gggcaattga ggttaagatt
    11341 aaggttaagg ttgaggttga ggttgaggtt gaggttgagg ttgaggttga ggttgaggtt
    11401 gaggctaagt aactgactat tgactgttta ctattgacta ttgactattg actactgact
    11461 actgaccact aaatgacatc cgcaaaactc ttctccgtcc tcctgaaatg ccggatccca
    11521 atccataaaa aaaacaaaca aatggccagt gagatcaaaa acccatccct gtaaaaaggt
    11581 tcatccagga ggcaccaacg gaatccgtcg atgacgccca ccatgggatt cagggaatac
    11641 cagaggcggt attgatccgg gatgacggcg ctgctgtatc cgaccggtgt gatgtacaat
    11701 ccaaactgga taataaacgg gataatatac ctgaagtccc ggtatttgac gttcagggct
    11761 gtcaggtaca ggcctgtgcc gaaagcggct ataaaagcca tcaaaataaa aacgggaagc
    11821 aataatactt tcagatccgg caggtactga aaatagataa acaggatcac caggatcacc
    11881 agggagatca acaagtcgac cagggaagtg attacggaac tggccgggat gatcatcctg
    11941 ggaaaataga ccttggagat caggtttgaa ttgcccacca ggctgttgct ggcctcgctc
    12001 agggcattgc cgaaaaactg ccagggcacc atgccggcaa agaccaaaag gatatacggg
    12061 gccgcccccg gcgattcgag tttggcgata ttattaaaga cgatgtaaaa gaccagggtg
    12121 gtcaacaccg gccggagtac actccaggcc aagcccagga ccgtctgctt atacctcacc
    12181 ttgaggtccc tccaactcag gatataaaac aattcccggt aacgccaaag atccatccaa
    12241 taatgccttt ccacggagtc cggcgtgatg gtgatttcgt cagttctcac tcggaggttt
    12301 tattaataat agtgaatatt cagggctaaa gcccaatcct gctgctgggt ttaagcccca
    12361 gcctaaaggc aggggttggg gatgagtacc tgatcgtcac acggaggtta ggagatcacg
    12421 gagttaagcc gtgcaggccc tgcctgacca gttctgtcaa taacttccat attgggcagg
    12481 gcatacaggg acccaaaaat taatgaagtt gctttatctc tataagttat ctaacaattc
    12541 ctctaactca atttcacctt gctgtcaaaa taatttttaa agtagttggc tggactggct
    12601 tgtgcgctgg tacggtaagc tttaatcttt ctccatgaac tcctttatgc aaacggatat
    12661 gactgccttt ttgtcgaacc actatccagc ccattctttt aagtgcattt acaatctcta
    12721 tatacgatag agagggatac ttcatatatt aatctcttca gtgaatgatt cctctaataa
    12781 ggattcatcc tccacttctt caaggtacaa ttcaatagcc tctttaatat tttgaagagc
    12841 ttcttccttc gaattgcctt cactaataca accttttaat acagggacat agacagtaaa
    12901 tcccccattt tcatttggaa taagcgaaac tctgaatttc ataaaatggc aaaatatata
    12961 tactaaagtt aaaagatttt atggaacatc ctgaaatggg gcaatcggca caaggaggcc
    13021 aggagtagac ggcgttcggc gcacggagga taggagggca cggagaaagg aatttttatt
    13081 ccattggctt taacccgtat ttttcttgta gcctctcatc gcatttgttt tcttatcctt
    13141 ttagggaaat gttacccata tatttctccc aggatcctgg caatggcttt agaagccggc
    13201 tcggcataag tatataatgg tttccagctg ccggcttccg gtccatgccc aaatacagac
    13261 cggaggcttt ccgtatccgg ggccaaccgg ttccacttgt tttcaagggt ctcgacccac
    13321 tcggtttcag tcctcagggt gatacagggc tttttcagcc aataagcttc tttttgcagc
    13381 ccccccgaat cggtgatcag tttataggaa tggttcagga ccttgagcat ttcaaggtat
    13441 gacaagggcc cggccaggtg tatattattt ggcagtttta agtccttcag ggcttgctgg
    13501 ctgcggggat gaaccggcca gaaaacccgc attcccaggg ttccaacagc ttcgatcagg
    13561 ctggccagcc tatccttgct cattgtattg gattgccggt ggatggtcaa aaggataaat
    13621 tgttcaccgg catcgaaggg taaaatattt tcgagcctga ttttttcttc tgcaagaggc
    13681 aaataacggg tgagggcgtc tgccatcaca tcacccactt catatacctg atcgtcgatg
    13741 ccttcccttt tcaaccattt tacgccctgt gcagaggaac agaaaaatat ccgggacagg
    13801 tgatcggtca gcacccggtt gatttcctcc ggcatggttt tgtcaaaact tctaagtccg
    13861 gcttcgatat gtgccaccgg gatgcccagg ttggctgcgg ctaaggcacc ggccagggtc
    13921 gagttggtat ccccgtaaac gaggaccagc ctgatcttgt ccaggaaagg cattaaatag
    13981 gcttccagcc gctcgagcat gagagcgatt tgtttgcccg ggctggcaga tccaacattc
    14041 aggttgattg ctggcgatgg aagttcgagc tccttccaaa aaacctcact catgttggcg
    14101 tcatagtgtt gacccgtatg tatgatgtac tcttctatac ccagtttttt taattcggcg
    14161 gaaaccacgg cggctttgat gaactggggc ctggctccga ggatggtggc gatcattatt
    14221 gcgggttcac accattggat cccacgaaga cacaaggctc acaaagaaaa aaagaagtat
    14281 aaaaaatcat ttcttcgata tcgccgtgtt ctttgtgtct tggcgggaca ccacaatcat
    14341 cacaaattaa aagggtgttt ggtcaatggc ttggctgcaa agggatggta atcccctttc
    14401 agtccggccg gcgccgcatt tcggatctca tagacggttt ggatcgaggg gcgggcgtct
    14461 tccagcccat aacctttccc gtccaggata tcccggtaac acaatgtatg cagttccgta
    14521 aacccttccg aaaagtcaac ttcctctcct tcgatggtca gggagcggta actgcggccg
    14581 tttttctcct taatttcttc gggcagcgtg gatgcttcaa tactcagaaa ccagcgtacc
    14641 cgggcttttt ccaggtccaa aaaaccggcc gcccggtcat gggtatgcag gtgcacggta
    14701 ttctgccgaa cgggaccaaa gatccagccc aggacatcaa aaaaatgcac cccgatattg
    14761 gtggcaatgc ctccggattt ggtgatatcg cctttccagc tggtataata ccaatgaccc
    14821 cgggcggtga tataacagag atcgatatca taaattttat ccggaggtcc cttcaatatc
    14881 ctgtctctga gttcgacgat tttcggatgc aacctgagtt gcaggatggt gttcacacga
    14941 tggccgcttt cggtttctat ttctttgagg gcgtcgatat tccaaggatt gaggacgacg
    15001 ggtttttcgc agatcacatc ggccccctgc ctcagtccga accggatatg ggcgtcgtgc
    15061 aggtaattgg gggagcatac acttatataa tccagttttt ttccctcccg ttttcttttc
    15121 tccaggtgac ggtcaaaacg ttcgaattcc acaaaaaaat cggcaccggg aaagaagctg
    15181 tccatgaccc cgacggaatc aaatttgtcg aaggctgcca ccaggacatt tccagtgtct
    15241 ttgatggctt tcaggtggcg gggcgccaca tagccggcgg cgccgatgag ggcaaaggta
    15301 ttcatggttg tgtggcgtct ttatggttgt tcgtaagcgg gaggcgggca gcatccgaat
    15361 tcccctccat ttccgatttt gaatatttat aacttatcca aaggctgaaa agacttagca
    15421 ggctgtaatt cagggaataa aacaacactg ctttagatac agatcccagt agatacaggg
    15481 aaatataaaa gatcagggca ttggatataa aaagatagag atcaatcaac agggtgatat
    15541 actgtttatc cacgatgagc atggtccggc tgaggggact ggtagtaaaa cgtaaaaaat
    15601 acataagccc catgatccgg gcaaactggc cggcttcttc ccagctttcc ccgaatataa
    15661 atacaaataa ccaaggtgca aacaggatca atagggcaaa gggaaggatc gaaaacaggc
    15721 ttaatgattt taaagtccat agaaagatcc tggaaaaacc gcccttctcc cggtatgcct
    15781 tggaagccct ttgaaaatat atttccccaa tcgggcctga aaccgctgcc atcggcagcc
    15841 caagcatccg cctgctcaaa ttaaaactgc ccgcggcagc gggtaaagac caggcattga
    15901 gcatcagaac agggacctga tgggtaaaat tatttatgaa ggaagcgggt aaactgaacc
    15961 tgaaaaaatt ggcatatttt ttagcggtag cccattgatt cagaaacaac tttttgaaca
    16021 agaaggcagg gtttttatat atcgtaccgg aaaacaagta cacactgttt gcaaactgac
    16081 tgagccaaag gccaagaaat aagccggtaa ggttacggaa ccaaagtcca aatccaatgg
    16141 aacaaaaagt ggataccatg gcagtcagta tattgccgga cgaaatcaat cttatttgcc
    16201 tgatccgaag cgcataactt ccgaaaaccg aggccagccc tgtgcataaa accgaaaacg
    16261 gaagcaacca gaaataatta aatgaaaacg gaaggggagg gacgattccc gcaaacatca
    16321 gcccccagat caacagaaaa gacaggatgc tgccacctac caggagttgg gcacaaagcg
    16381 ttatgagatt caccgcttcc tcatcatttt tggcaaccac gatggcttga ttaaactgta
    16441 aagtggctac caggccaatc agtccagata tacttatata cagacccact aaaccataat
    16501 ccgaaggctg ataaatacgg gtcaggatag gtgcagccag gatgggtgcc acctgggaca
    16561 gcagtgatcc ggtcattagt tttgcgatat cccttacggc gccggatgaa gcagacaggc
    16621 ggtattggct cctcagccag ataacaatct tttgaatcat gatagtgatt tccgggccag
    16681 ttcccggtac agttcgaaat attggccggc aatgaggttc ccgttaaatc gtttcacatt
    16741 gtcaaatcca ttcctgatta attgattcct gtaatccgga tcagcaatca ccttccggaa
    16801 accattgcga atggattcaa tatcccaggg gtccaccagg caagcggcat ccgcagctat
    16861 ctccggcatc gaggaggtat ttcccgtaac cacgacccgg cccgtggcat ttccttcgag
    16921 gattggcatg ccaaaacctt ccaccgtgga ggcaaaggat acgatgtcgc aggatttata
    16981 caagtcaaac acttccgcat ccgtatccac cctgtcaata tgttcgtatt ccagtttatg
    17041 ttttttcaac aacgcggaat gcgcttccgt cagcgggcca aggatgcgat aacggcagga
    17101 taatccttcc agggcgggta tgatcctttc caggttttta ttccgggccg tgccgacctg
    17161 caatatcaat ggcctggcct cattaaatgg cttctcgctg ggacggtaca ggtcggggat
    17221 atttacataa atcaccctga tcctggccgg atcgtaatgg atgtgctgca acagatcatc
    17281 ccgggtcgcc aatgaaatca cggtgataaa agccgctttt ttcgcaggaa gggtaaacca
    17341 gaaatactta aacagtttta acctgatgcc gctcagttct ttaagccttc cgcaatcgag
    17401 tatggtcagc aaggtctttt tccggttcat caaaagggtc aaaaaatgca catcgccggt
    17461 cacatggttt acctgttttt gcgaaaaagc cgcttcgatg acattataga tcctgcgcca
    17521 gaggcctctg gactcaaacc gggatatttt gagatttatc cggatatcgg caggcgccag
    17581 ttcgcggata cttttaaaat aactttcaag actgtagttg ccgaagggcc tgggttttcg
    17641 ttgaaaatgg accacttctg tcatgccttc ctgtgggctg attcccaatg gataaaatcg
    17701 atttcgtcct ctataagttt cgcgatcaat tcgtcgttgg acagctcgta tttccatccc
    17761 agttccagcc ttgccttgga aggatcgcca tagatttcaa agagttcgtt ggggcggtac
    17821 agcgccggat cggttttgac ataccgctcc ttgtcaagcc cgagccggtt gcaagccttg
    17881 tcgatcaggt catcgaggga catgacattc ccggaacaaa ccaggaaatc atcggcagaa
    17941 tggtgctgta aaatacggtg catggctgcc acataataag gcgcgtaacc ccagtccctg
    18001 cggatggccg tattgcccac cgtcaaaggc tgatctgtcc attgcagctt tattttcacc
    18061 aggtggttga tgattttttt taccacgtaa ttcatgccac ggagcgccga ttcatgattg
    18121 aacaggatcc cattggctgc aaacattttc cgggcttccc ggtaattgac caccaaccaa
    18181 tgggccgcag ccttcgaaat gccgtaaggg ctggccggat ggaacatcag gttttctctg
    18241 aggggcagtt tattcctgtc aatgtttcca aacatttcgc tgctggaagc ctggtagaat
    18301 ttaacggctt tgttggtttc caggatggct tcaagccagg acaaaacgct cagaatattg
    18361 aattgtaccg tggcataggg ttgctcaaaa gaaaggccga ccgaactctg cgcggccagg
    18421 ttgtacactt cttccggacg gacttcttcc aggatgccga ccaggcttgc cctatccatt
    18481 tcccgacatt caatgagccg gacctggtcc aggatgccca ggtatttgag cctgaaatcc
    18541 tggaaagtgg tgagagaccg ggtaatgccg gtcacccggt aacccaatga tatcaggtat
    18601 ttagcgaggt aggcgccgtc ctggcctccg atgccggtga tgattgccgt ccgggttgtt
    18661 gaattgttga attgtcgaat tgtcgaattg ctttgcaagt ataattatca gatatgttgt
    18721 ttcatgatat gctgccggga gccattgccc cagcttccag ctcctatctt ccagcttcta
    18781 actcctaact cctagcttcc aacttccagc ttcctgccat tttgatcgcc caataccggg
    18841 cccagctgag cctgccctgc ttaaacgcct gaaagtattt ttcctcctta ggggtctggg
    18901 gcctgacctg ctcacttgtt ttcatccata attcaaaagg aagggtgaat ccctgtttgg
    18961 gccggttcca aatggcttcg ggcagaatat ctttacagga ttcaatgagc agaaatttag
    19021 gctgtccggg tttgaaccgg atatcggagg gcattgtatt gaccagttct acaaattcct
    19081 tgtcgagaaa aggcacccgg atctccaggc tatgccacat gctcatataa tctgcatctt
    19141 tcagcaattg gttgtgcata taggcatggt attcgagcca actggctttt tccaaccggt
    19201 cctccccgtt ccaggaggcc gtctgcatgc tgtcgagctg gtgtcggacc tctgtttcat
    19261 ccgcttccag gatgtccgct atccggtcta tggtatgtaa tccccggtaa aacaaatatt
    19321 ctccggtgct gttttttaaa gtcgcaaatt ttattttacg gtaacggtcc gaagtcccga
    19381 gcgttgctaa ttccagaaca gggccaggac attttaccag ccattgccac caactggaat
    19441 tcttcacgga aggataacct ccaaacaatt catcagcacc gattcctgag agcaccgatt
    19501 tgagcccggc ccggtgcgca tagccggata tcatatacac attgatcgcg tcggtactcg
    19561 gctgatccat ggctcgcagc gcatcgggca gttgcagatc gaaatccgcg gaggttacga
    19621 catattcatg atgcctggag ccggccattt gaactacctg gtcctggtat tttttttcgg
    19681 aatatccgga ttcgttaaag tggacggaga gggtatgcaa atgctccttt acccagggtt
    19741 ttgccaggag ggtcaacagg cttgaatcaa tgcctccact aaggaagatg cccaatgggg
    19801 cgtctgagat caggtgcctt cgaatcgcct gttccagggt cgagcggacc cttaaagccg
    19861 cttcaccggg tgcaaccttt tcttttatat catgaccggg aataaagcta tgcagcttaa
    19921 tcctggactg gccgatttca aactccagta tggaaccttt agccagggat tgaacctgtt
    19981 gcagtgtcgt gcggggggca gggatatggc cgaaggccag gaaaaagatt ttccagtttt
    20041 tgtcttcagg ccagtccggc tgcagggccc ggaatgcctt gatctcagaa gcgaaataca
    20101 ggttcccgcc ccggacagcg tagtacaggg gcttaatgcc ggcatgatcc ctggccagga
    20161 tcatcgaatt tttcctttta tcccagatcg cgagggcaaa catgccgttc agcctgctga
    20221 aacccatgtt accccattga aggtatgcct gcagaatgac ttccgtgtcc gatcgcgttt
    20281 taaatttatg tccaagcgcg gtgagttcat cccgcaacgc tacatagttg tacacctcgc
    20341 cgttataaat caaatggact tgtccacctt catccgacat gggctggtgg cccccggggc
    20401 ccaggtcaat aatggacagc cggttgtgcc caaggtaaac ccgggaatct tcatccacga
    20461 aggttccctg gtcatcaggg ccgccccggt acatactgtc ccgcatccgg atcagatcgg
    20521 taagcttgag accggggtca ataaagccag ccaggcggca cattggagaa ttaaattaaa
    20581 gaattaagaa ttaaggtatg ggatgcactt tgtcattctc cagccggtaa agctgatttg
    20641 tttccggaca agtagccaat ccctggttat cgaaaaacag tttatgcccg gccctgctca
    20701 tccaccctgc ctggtatgcc ggattaccga tgaccagggc atagggggga acgtcgcggg
    20761 tgacgaccgc ccctgcaccg atgaaggcat attccccaat ctctatcccg cataggatgg
    20821 tggcattggc gccgatggta gctcctttac ggaccagggt tttctgatat tcatctttcc
    20881 tttcaatcgc gctgcgggga ttgattacat tggtaaagac catggagggg ccgagaaaaa
    20941 cttcgtcttc gcaggtcact ccggtataga tgctgacatt attttgcact ttgacccggt
    21001 cacccagccg cactccgggc attaccacta cattctgccc cagggaacat tcctttccaa
    21061 tcacacttcc gggcatgata tggcagaaat gccagatgcg cgtgccttcg ccgatgatag
    21121 ctccggggtc gatgtaggag gagggatgga tcatggttga ggctgaggat gaggttgagg
    21181 ctaaggctaa ggttgaggtt gaggctaaga ttaagattga gggtgaggtg gaggtggagg
    21241 tggaggtgga ggtggaggtt gaggttgagg ttaacggctc aaccttaacc ttaaccttaa
    21301 ccttttagag ttttaataat tttattcaat tcatgaatga cctcatttaa ggattcgcaa
    21361 attttatttg tttcctcttt tttaaaatat tcaactctga gcccatattc caaacaattc
    21421 tgtgtttcaa acgaagaatc tctggaaaca atgtaataat atgctttatc cctattattg
    21481 tgccttccaa atccttccgc aatattgttt gatacgctta acgccgctct tcttatttgc
    21541 gaagtaagtc cataatcctc tgcacgaggt aaagatttgg taagaataaa aatttccgtg
    21601 gccacatgca tagctttttg ccaaaccaac atttctttaa aggtgatgta tttcatagtg
    21661 tagtgtttta aaatccgtaa aatttgcaaa tatcccaacc ttaacctcaa cctcaacctt
    21721 aacctcaacc tcaacctcaa ccttaacctt aacctctacc ttaacctcta cctctaccca
    21781 agcacccggt ctgcgatggt cttgccgatc gacaaactcg aggtggcagc aggtgaaggc
    21841 gcgttgacca catggaggag ctgatcgttt tctcggaaca ggaagtcctc tacaaggttt
    21901 ccctggcggt caacagcctg ggcccttacc ccggacccac ccggtgccag gtctttgctt
    21961 tgtatttccg gtaccaattt ctgaagtgcg tttacaaagg ccggtttgga cagcgaccgg
    22021 tacatttcaa atatcccgtc tttccagtat ttgcctgcca gctttctgaa accgggatag
    22081 gacagggtat cccaggtgtc tttccagttg aagattttcc agttataggc ttcacggccc
    22141 agggcccaaa ccgcattggg tcccgcttcc acgcccccat gcaccaggga tgtaaaatgc
    22201 accccgagga atgggaaatt gggattcggt accgggtaga tcagggtttt caccaggtga
    22261 tacgcttcgg gccttagcgt ataatattct ccccggaaag gcaggatccg aagatcatag
    22321 gaagggtccg tcatgcgggc cagacgatca gaatgcaggc cgccgcagga gatcacctgg
    22381 ccggcttgcg tttcttcttc agctgttttt acagaccaac ctgaatcgtt tttctcaatc
    22441 gtggtgaccg gagatccgca gatcaccctg ccccccgctt cttcaatttt tgattttaat
    22501 ttcagggcca ccgatttgta gtcgatgatg cccgcctggg gcacccagat ggcttcgata
    22561 ccctccgcgt ggggttcaat ttcctttaat tggtcccggc caatgagctc caggcctttc
    22621 atatcgtttt gaattcccct ttcgtagatt tttttaaggc cgggcctttc ggactcttcc
    22681 gtggccacga tcaccttgcc acaaattcga tatgggatgg catgttcatc acaaaattgc
    22741 agcatcattg cataacccct caggcagttc agggcctttt tgctgccggg gcggtaatag
    22801 atgccggaat ggatcactcc gctgttgtga ctggtctggt gcatggccac ggacttttcc
    22861 ttttcatata ccctgacccg gagatccggt ctggattgca acaactggta tgctgtggcc
    22921 aggccgacaa taccaccgcc aattattgtc acggattcct gaaccattta tgtgtgaaat
    22981 aaagaattaa agaaattaag aattaataaa ttaagggaga gttcttaatt ctttaatccc
    23041 tttaattttt ttaatcaatc taaagtttcc aatactcaaa gccctcccgc cattcataaa
    23101 tgattccctt gagatccagc aggagtggga aggccgggtc gagcaggtct ttataatact
    23161 ggaccggatt cgattgaaac tgccggtggg ccacggcgac gatgacggca tcatataccg
    23221 gggtttcccg gctgctcaat tcgaagccat attcccggga gaaagccccg gggtccacat
    23281 tgggatccac ggcctccacc tggagagaat actcaagaag ttcgtggatc aaatcggata
    23341 cttttgaatt gcggatatcc gaaacgtttt ctttgaaggt gacgcccagg atcagcaccc
    23401 ggcagtttat aggattcttt ccggcggcga gcagtttctg caccagtttt tttgcgatat
    23461 aagccggcat ctcatcgttg atccgccttc cggaggcgat gacctggggt tccaatccga
    23521 gcgccttcgc cttatgcaaa aggtaatagg gatccacccc gatgcaatgg ccacccacca
    23581 gccctggata aaattttata aaattccatt tggtagccgc ggcttcaatc acctcatggg
    23641 tatcgatttc catcaagtcg aagaggatgg aaagttcatt catcagcgaa atgttcaggt
    23701 cgcgctggat gttttcaatg acctttgcgg cttccgccac tttaatggaa ggagcctggt
    23761 gcaaacccgc atggatgatg cttccataca atgcggtgat attctccagg gcttcctcat
    23821 ccgatcccga gactattttc aagatggaag ccaggggcct cgttttgtct cccgggttga
    23881 tccgttccgg cgaatatccc gctttaaaat cctggttcag gcgaagtccg gatacctctt
    23941 cgatgacagg gatacattct tcttcggtac agccgggata gaccgtagat tcaaagatca
    24001 cgtagtcccc gggtttgatc acctggccga taaaaccgga ggcggtgcgc aggggtttga
    24061 ggtcgggcac tttgttttca tcgatatccg tcggcaccgc gacgatataa acctgacagt
    24121 cgcggaggtc atccaggacg gaagtaaaga ggatgtcccg gccttcaaag tcagccgcgc
    24181 ggacttcacc ggacgggtcc tcttttccct gcatcaatgc cacccggtcc ggattgatgt
    24241 caaaaccgat gacccggaaa cgccttgcaa actcgagcgc cagcggaagg ccgacataac
    24301 cgaggccaag gacggcaatg gtggagtctt tgttttttac agattcaaac atcgacaggg
    24361 tcaggtataa atcaattcct caaatccgtt caataaggga tgatgcacca ggttgttatg
    24421 ccataaggtg acccattctc ccccgtgttt tttacattgg tccttcagcc tggtcaatcc
    24481 ggagtccacc ggttttttct ggtaaaaaac cagggtcgca tccatcagca gcaggggctt
    24541 ttcccgaagg cgaagctttt tcctttcaag gcaatcatat acggtgaaag tgtaacaggt
    24601 cccgcaacgg aatcccggtt ggcccgaata agccagtgaa ctctcccatt cgaaacctgc
    24661 ctgatcccat aacctccagg taaagggtac cctgaaccgc atgaaatgct ggcggccgct
    24721 tttaattttt tgacggacga ggttttgcag ttcagccagt tcctgattaa aaggttcagg
    24781 ttccaggtat cggttataac ccgggtgaaa accaatggag tgaccagcat cctccacgga
    24841 acggatcaga gagaccagtc tgtggtcctg gctggacaag gcttcgtcgt acttgctgct
    24901 gcggcttatt aaaaaattaa aataaacttt caagccatac ctttccgcga ggtccagcat
    24961 atattggtgc tgatcgaaag gatcaggctg gttgaagaaa cgggtccgga ccaagctgcc
    25021 gaggttatcg gaaaacatcc tgaggtcccg tcttttcaag aggtccccac ccagatgctt
    25081 caggctggta taccagtccg gccattgata aggttggtcc acatcgtggg tgaccaccgg
    25141 ggtatacata tgatgttttc ttttaccgac ataacccgct ttcaaaagga gcagccagag
    25201 caggtcggcg tattcattga ctaccggcct gtcgagaaac tgataacgaa aagccaggga
    25261 aagcgaagcg gttgaccggt cgtgttcatc cttctgatcg tccacgacct cttcccagcg
    25321 gctgagcata aaataagtgg aagcgatgag gtctgcatga aggacataag cctcccgggt
    25381 gtcctccaat attccttcgc catagatcac cggcaggttt tttaatgacc attccgggtc
    25441 agaccaggac atgatcgcgc cgggtattgc ttctttcctg atataacctt ccgattcttc
    25501 gtaccggatg aaaaactgat ttttgatgat tatcgttttc aggcccggtc ctctgatctc
    25561 gaaatccttt aatcccgcat ccatcctgaa actgcaggaa aggtccagga attcttccag
    25621 caggatttca aaagtatatt ttgtttccgg ataaaatttt tggggaaggc agatttcgag
    25681 caatgtcaat cagttttcat aggttaggct gtgatctccg tgcgttattt gcattttgct
    25741 cccttcgccg ttcgcctttc tcccttcgcc cttcaatatc attccgtgtc aataaaaaat
    25801 ctccggaccg tatcgatcac tttttcctgg tcgtgcgcct tcatctccgt atggatgggc
    25861 agggagatca cctccttaca gaggcggtcg gtcaccggga ggaaatcgac cttgccgcgg
    25921 aagggtttga aggcctcctg atcataaagg gggactggat aatagatcat attcgggatg
    25981 ccctcggcag tgaggaaaga agccagggca tcgcggcggc catctaccac cttgagggtg
    26041 tattggtgat aaacatgggt ggaatgcggg gattccgcag gagtgcagag aaagggaata
    26101 tctttaaacc cttcgttgta cgcataggcc acgttctgcc tggcccggca atattcatcg
    26161 agatgaccta atttgatcct gagcacggca gcctggatgg aatcgagccg cgaattcact
    26221 ccgatcaggt catgataata tcttttgcgt tggccgtgat tggcgatcgt gcgcagtttt
    26281 tcagccaggg cttcgtcgcg gcacatcatc gctccgccgt ctccgtaaca gcccaggttt
    26341 ttggaaggga aaaaagaggt acatccgatg tggccaaggg tcccggcctt ttgtttttta
    26401 ccgtccgcga atacatagtc cgctccgata gcctgcgcat tgtcctcaat gacggggatg
    26461 tggagttttc cggcaagatc caggatgccc tgcatttccg cgacctggcc aaaaagatgc
    26521 accggcacga tcgctttggt tttgggagtg atggcctgac ggatgagttc cggcgtacaa
    26581 ttgaaactgt cgtaatccac atcaatcatc accggacgga gttgcaacaa agcgatcact
    26641 tccgcggtgg ccacataagt aaatgcaggt acgatgattt catcccccgg cttgcagtcc
    26701 agggccatca aggcgatctg tagggcgtcc gtaccattgg cgcagggaat gacatggggg
    26761 atgtccaggt acctttccag ggcttgctga aactccctga cctcaggccc gtttataaat
    26821 tgggcgtgct ccaggacatg gagcaccgcc tcgtcgattt ccgttttgat gcggtggtac
    26881 tgggatttga ggtctaccat ttgcataaca tactgtttta taaggttgaa atggtttttc
    26941 ggccaatgct ctcataataa aacccagggg gaatatcgtc caggtcatac agattccgcc
    27001 cgtcaaaaag agccgccgtt ttcataaggg atttgatccg atcaaattcc ggggcgcgga
    27061 acaggctcca ttcggtcgcg atcaccaaag catctgcctg gtccaggact tcgtattcgt
    27121 tttccccata ctggatttga tccccatacc tgtcttttac atggtccatg gcttcagggt
    27181 caaagaccct cacctttgct ccccgggcaa gcagatcatc aatcaccgtc aaagccggcg
    27241 cttcccggat atcgtcggta ttgggtttaa aggccaggcc ccagatggca atttgcttcc
    27301 ctccgaggtt ttgcataaaa tatttttcaa gctttgccgt caggatgtgt ttttgatcct
    27361 ggttggttct gaccaccgaa ttcaggatat tcatttcata atcaaattcc cgggccgtct
    27421 ggatgagggc catcacatcc ttggggaaac aacttccgcc ataaccgatt ccgggaaaca
    27481 agaaccgttt acctatccgg ttgtcgcttc caatccccct gcgcaccata tccacatcgg
    27541 ctcctgcccg ttcacagagg ttggcgattt cattcataaa ggtgatgcgc gtggccaggt
    27601 aagcgttggc tgcatacttg gtcatttccg cagacctgag gtccatggtc agaatgggat
    27661 tgccctggcg tacaaaaggg tcgtacaaat gtttcattct ttccagggct ttttcgctgc
    27721 tgcttcctat gaccacccgg tccggtttca taaaatcctc caccgccacc ccttccctta
    27781 aaaattccgg gttggaaacg acatcaaaca aattattatc cagtttatct cccagcacag
    27841 cagccacttt ttcagcggtg cccacaggta ccgtgctttt attgaccacg atcgtatatt
    27901 tttgaatcag gtcccccagt tgatccgcag tcatgatgat cgcgctgagg tcggcggagc
    27961 cgtcttctcc cggcggagta ggcaaggcaa gaaatatgat ttcggcgtga tcaaccgctt
    28021 ttttcagttc ggtggtaaaa gtcaggcgtc cctggcgggt gttcctttca aaaaggtttt
    28081 ccagccctgg ttcataaatc gtgatccttc cctggttcaa ccggctgacc ttttgggcgt
    28141 cgatgtccac acaaatcacc tgatgcccca tttccgcaaa gcaggtaccc gtgacaaggc
    28201 ctacgtatcc ggtgccgatt actccaatgt ccattttatt ttaattaagg aattaaagaa
    28261 ttaaagaatt aaggagacga gatctgcttg gaagatttta aaattgcatt taatatccta
    28321 ataatttctt ctgcatgcgt taaaagtccc gttgcctttt cttcatccag gtatcctgaa
    28381 tcttttaaca gcctgatcca ataatggact tcttttgctt ctttatagga tatctggaat
    28441 cttgcaataa aatccctttt gctatgcccg gctaatcctt cctcaatatt tgcacctatc
    28501 gaagtggcac atctcaaaaa ttgttttgat aatataaatt ccttcttatc aacaattagt
    28561 atcttataaa tccggataga atccaaagag aactgatagg atttctctct tataatattg
    28621 tccttcatag tgggtgtgtt ctttcagcga ataaatatcc attaatcctt aatttttaat
    28681 tattaatttt taattttttc catggcttcg cccccgatct gtcgcagtgg aagatcgcaa
    28741 ggaagaatat ccttcaatga ccggggatca ccgaaggctg cggagatggg ccggatcgat
    28801 ttggatacgt aatccaaatc cgatgcggtc cagttcgatc aacagctcgt ttttaccgag
    28861 cgagcggatc agtttgcccc tgagtccggt caggttgccc aagaccactt cgactgcctc
    28921 cccggggctg aaaggccggt tttcaagaga gatcccgttg atttctcccg ttacgcgctg
    28981 gagccattcg atttcctggt ccgggatggc aatcaggttt ttattgaagc ggacaaagcc
    29041 ttccacatgc tgggtttcca gcacgggcac gtactcggct ttgcggatgt ggacaaaaac
    29101 gtagcggtag atcagcggga gcatctgggt ccttttttta cgggtgtaca cccggaccac
    29161 tttttggagg ggaatataat ttttaatacc tgcccgggtc aacgcctggc tcaccgcttt
    29221 ttcggccttc accctcgtga ggacggcaaa ccatttggtg gtatccgggg acaaatcgtt
    29281 ctggcaggcc gctgcaatca tatcaggggt ttaattcatt ttgaggattg actggatgag
    29341 gctccaggta gggcgcggac cagatcagat atggctccgg atgaaaggaa ctccagccaa
    29401 tcctaagctc ctcctcatta tcgtaaatca cgtaacggat cttgcgtttt atcagttttt
    29461 cggatttttc cacgagctgg atcaggtagg acttgtccaa ctggccgacc aggatcagat
    29521 caatcacccc actatccctg ccctgtgcga aagtaccggt aagaaaggcc cgctccagca
    29581 ggcccagtcg tttgaggatg ttgtccagga tctggtcgat cccaatatat ttaagaagga
    29641 tactgtggat ctcctggtac aagggataga ggggattggc tttaaaatac tttttgttgc
    29701 cttcctggaa ggagttgagc atacccgcat cttccatctt attgagttcc agccgaatgg
    29761 cattggtaga ttctccaaac tccgattcca ggctgcgcag gtaggaggtc gtattgctgt
    29821 tgaggaaaaa cttcaacaac agtttaatac gggttttgga agagatcagg tcctcaatca
    29881 agattgagta gttttattac tcaaatataa atggaaatct tcaggatttt attgcttcat
    29941 ggaccagatt ctttcccagg atccaggccg aggctaaaac cccgcccagg atcaggccca
    30001 taaggagttg acggagccgg tttggggcct gggcgtccag gggaggaaat ggacggtcta
    30061 tctcccggat aaaaggcgta ctgtttttaa gggcgtaatc cgcaatttcc aggtttttca
    30121 gcgcctcccc gtaggcaatg gcatacatgt tggattgttt gctttgaatg gtcttccggg
    30181 tgcggtcggt tggagcccag agagcgcctt ccgactcgtc catgttgctg atggcgtact
    30241 ccgaactggt ccacctttcg tagatcgaat ccgccttggc tttcaccagg tcgaaggttt
    30301 gtttttgttt atcgctggat tgctggatgt aaaaccggga aaggtggtaa aaaatccggt
    30361 cggtgagggc cagggaaagt tcttcgtttt cggtctgaga gctaagcgaa aagatagtgc
    30421 tttcgggttt ataggtgcaa gacagcaaac ccgacttttc ccctcctacg gtaaatccat
    30481 aaaccctttt cagggccgcg ttttcaagca aactgaatcc ggccagtgaa tcggagcgga
    30541 accggaaatc cctataggct tcatcggcct cagcccattt tttatccagt gcatagatcc
    30601 ggatgatgtg gtttgcgagg aaatcttccg ttccccccac cgccgcctta tcaaacaagg
    30661 ccgcattgac aatgcgcatg gacctggaca attccacaat cttatccagg gagaccttgc
    30721 cggtgccgct gccccccagc ccaaactggc ccaataaacc gctgatggaa ctgtatccca
    30781 gattttcccc ttcgttcagg gcaaaagtga gtgtcgcatt aaaggtgacc ggttggaacc
    30841 gggcttttaa atacatcagt gttccggtca ggagggacag tacgagtatc agataccttc
    30901 ctttccataa aagcaggacg aatccctttc ctttgacgat gatgtctttt aaactgaggt
    30961 cctggtcgta atatgcgcct gggatctttg gatcgtttat attgatttgc tcaggcatcg
    31021 taatttaaaa gcggggaaag ccatttttcc gtaagactaa tatcctggtt tttccggagt
    31081 gcatactctt ccacctggtc ccggccgatg ttgccgaggc caaaatattt agactgggga
    31141 tggctgaagt accagccgct gacggaagag gccggatgca tggccatgct ttccgtaagg
    31201 ttcatgccga ttttactctc tacgccaagc aggtcaaaca gggtttgttt ttcggtatgg
    31261 tccgggcagg cggggtagcc gggtgccggc cggatgccct ggtatttttc agcgatcagt
    31321 tcttcattgg tcagcttttc atcactgtaa gcccaccact ccgtccgcat ccggtaatgc
    31381 atacattccg caaaggcttc cgccaagcgg tctcccaggg atttgagcag tatggcatgg
    31441 taatcgtcgt gtccggcctg caattcccgg actttttcat cgagaccgat ccctgccgtg
    31501 acggcgaaac ctccgatata atcggttttc ccggatgact tcggcgcaat aaaatcggcc
    31561 agggacaaat aagggatgcc ggctgacttt tcccgctgct gcctgaggtg gtgggctacc
    31621 gcgacgacct ccccggcttc attccggatt tcgatatcat cggtttccag gctgttcgct
    31681 ttgaaaatac ccacgattcc ttccgccctg atccattttt catcaatgat gcgccgaagg
    31741 aggctttggg catccaggta aagttttttg gcttccacgc ccaccagggg atcttcaaag
    31801 agggcgggat atttgccatt gagttgccag gaagaaaaga aaggagtcca gtcaataaaa
    31861 tcactcagtt cttccaggga gtaatccagc aggactttgg tccccagcca ctttggtttg
    31921 ggaggttcgt aacggtccca gtcgatgccc agtttttgtt tgcgagcagt ctccagggaa
    31981 acgtaggttt tggtggtctc ccgctccgac cgcgatttcc tcacctgggt gtattcatcc
    32041 ctggtctttt caataaaaac ctggcggccc acttcatcct tgctcaacag ggcgcttacg
    32101 acagccacac tgcgggaggc atccaggacg tggatgaccg gagcaaggtt atactggggt
    32161 tcaattttga tcgccgtgtg cagtttggac gtagtggcgc caccgatgag caaaggcagg
    32221 ttcatgcccc ggcgttgcat ttcttttgcc acatacacca tttcgtccag ggaaggggtg
    32281 atcaggccgc tcaggccgat gatatccacc ttctccgcca gggcagtttc caggatcttg
    32341 tcgcagggta ccatcacccc cagatccaca atggtatagt tgttgcagcc cagcacgacg
    32401 cccacgatgt tttttccgat atcgtgcacg tcgcccttta cggtagccat caggatcttg
    32461 cctttggaac tgtccccggc ggattgtttc tgggcttcga tgaaaggggt gagataagcc
    32521 accgccttct tcatcacccg ggcgcttttg accacctggg gcaggaacat tttaccggat
    32581 ccgaaaaggt cgcctaccac gttcatgcct tccatcagcg gcccttcgat gacggacagg
    32641 gccatggggt attccttccg gatctcttcg gtgtccgtct cgatgtactc gtcaatccct
    32701 tttaccaggg aatggatcag gcgctggcgc accggttcct ttcgccagga ttcatccttc
    32761 tgcaacactt tgccgccccc tttgatccgg tcggcgtatt gaatcaatgc ctccgtggcc
    32821 ttttcattcc ggttaaataa aacatcctcc accagcttga gcaggtcctt gggaatttct
    32881 tcatagactt cgatcatacc ggcgttcacg atacccatat ccatgcctgc ctggatggca
    32941 tggtacagga aggccgaatg catggcctcc ctgacgtggt cgtttccgcg gaacgaaaac
    33001 gaaaggttgc tcacgccccc gctgaccagg ctgcccgggc agagtttttt gatctggcgg
    33061 gtggcttcaa tgaaattgat ggcatattca ttgtgctctt cgatgccggt ggcgatggca
    33121 aaaatatttg gatcaaaaat gatgtcctgg ggtttgaatc ccacttgttt ggtcaggatg
    33181 tcataggccc ttttgcaaat cgacactttc cgttcaacgg tatcggcctg tccctgctcg
    33241 tcaaaagcca tgaccacggc ggcggcgcca taacggtgta ccagtttggc ttgccggata
    33301 aactccgctt ccccctcttt catggaaatc gaattgacaa tgcctttgcc ctggaggcat
    33361 ttaaggccgg tttcgatgac cttccatttg gaggaatcca ccataatggg caggcgcgcg
    33421 atttccggtt cggcggcgat caacaataaa aactttttca tggccgcttc cgaatcgagc
    33481 aaaccttcat ccatgttgac atcaatcacc tgggccccgc cttccacctg ctggcgcgcc
    33541 accgacaagg cttcggcgta tttgtcttct tttatcaggc gggcgaaagc tttagacccc
    33601 gtgacattgg tgcgttcgcc gatattgaca aaattaaggt cggggtgaaa aatcaatggc
    33661 tccaggccgg acagttccgt gtatggaggc accttgggaa tgttccgcgg ttttaaccct
    33721 ttcacgcttc gggccatgac ccggatatgg tcaggcgtgg tcccgcagca gccccccacg
    33781 atattgacaa aaccgttgtc cgcaaagtcg cgcatataac tttccatttc ctcaggggtt
    33841 tgatcgtaac cccccaattc attgggcagg ccggcattgg gataggcgct caggtaacag
    33901 tccgccactt tggacaaggc ttctatatgc ggccggagtt ctttggcacc cagtgcgcaa
    33961 ttgagtccca cactgaaaag gttggcatgt tttacagaaa tccaaaaggc ttctgcagtc
    34021 tggcccgaca aggtgcgtcc gctggcatcg gtaatcgtac cggataccat gagcggaagc
    34081 agcttgccgg tgtcctccag atattgctga acggcaaaaa gggctgcttt gcagtttaag
    34141 gtatcaaata cggtttccac cagcaggagg tcgactcccc cgtccaccaa tccccggatc
    34201 tgctcgtaat atatggtgac cagttcgtca aaatagatgg accggaatcc cggattgttg
    34261 acatcgggag aaagggaggc cgttttattg gtcggcccga tggcgcccgc cacaaaacag
    34321 ggacggtagg gatgggaggc caggtattct tccaccgcat ttttcgccag ttgtgctgaa
    34381 cagaggttca actcataagc caggccctcc atatggtaat cggccagcga aaccgcattc
    34441 gcattgaatg tattggtttc aataatgtct gagccggctt ccaggtaggc cttgtggatg
    34501 tcccggatga tcttgggctg ggtcaggctg agcaattcgt tattgccttt tacgtcatag
    34561 gcataatcct tgaagcgttc gccgcggtat cctttttcgt ccagggcatg tttctggatc
    34621 atggtgccca tagcgccgtc aagaaccagg atgcgttgtt tgatgatctc gtgtatggag
    34681 tcgctcattt tgggtgagca aaggtaagga cttgaatgct attaaactat ggactttggt
    34741 ccctgtacct tttatctatc tccttggata gttggccggc agaatgtccc tgaccggttt
    34801 taaagccgtg gtccattcac tcccttgcca tggccaaata tgcctcgcgt gaagaataag
    34861 ttaggaacat gctttgagca tcttttttta cgaacccgtt tacaactgaa aagtatgcac
    34921 aaccttcaac aggagggccc ggtattcgga ataagggaga tgggggtctt tcaggccaag
    34981 gtcgttaagg ctttcgttgt agaccaggta aaatcatttc cgtcctttgt attgtaacgg
    35041 aggcggaaat tcagggcctt gacgcgggtc aatgaattga cctgcacaaa agtgttgatg
    35101 ctcagtttca cattcaggga agtggcgatc ttcatgcggg caacatgggc tatataatcg
    35161 ggggatgctt taaaccggat ttggttaaat tggtaaaatc cactcagggt caggtatttc
    35221 gaaaagatgc agaggggacg gtcaattgaa aacgaaggct gctgaaaggg atgcgtgctt
    35281 ccatcatcca tccttcccca ttccttgtga ccttggcctc ccaatacgaa ttccaggaca
    35341 gatccacgga gagttcgccc tgcccgtcat tacttacact cacatcgatg cggaaaccgg
    35401 tcggggaaac cacaaaaagc aatccgtttt cctggtcttt ataggtatcc agcaatatgg
    35461 cgatctggtc catggacagc ccacatttat cccgttcaaa ataagatccc tggatctggc
    35521 ccgggtcatc gtaacatatg gcggagaaat aaagatattt ttcatcgtaa gccatgctga
    35581 accgggtcct ggtattgggc ttattggaat aagaaggcca gtggctcaca aagtccaggg
    35641 tatctgaacc ccattccagg ggatcgacct gtccgtcaaa atcgatggta ttccggagcc
    35701 ttgatatgct ggtcagggtt gccgcctttc ctgatttatc gtttgttgga actgggttgg
    35761 acatttccat cggttgaccc catgaaatga cgcggataaa agaaatagcc agccagatcc
    35821 tatgtttcac acctggagtt aaaagactga tttaaatgat tcaaaacatc ctggatccat
    35881 ccagtttcac ctccgcgtat tgccagcttt gttgcaggtc tttttcaata gcagccagtg
    35941 cctttgggtc ctttttcaac tggtacgctt ttttaaggcc atgttgtgcc catccattcc
    36001 tgggcagcca ggcaaggtct gccttatacg tttcgatcgc ttcgtcatag tggccgcttt
    36061 ctaccaaaac cgcacccagg tgatgacgga ctgaaaaaaa ccagtcggga ggttcgttgt
    36121 aattcagggc atcttcgatg gaaacagcct cttttagcag tgttatgctt gcatcaaaag
    36181 atttttcctt tgccagtatt tcggcttgta aaactttgga ggcgatggcg acaatgtccg
    36241 aaatgcggtt gatcccccag atggtgagtt cttgcagact gctgtcccgg gccagggaat
    36301 cgagggtgct cagctcccct ttcgcttgtg aaagttcccc cttgccaagc cgggcaaagc
    36361 ccctggcgta atgccggatc gcctgcacat aagggagcat cgagggctcg ggttggataa
    36421 gaatggaatc ccattctccg aattttacct gaacaaaata agggatggta taataatgct
    36481 gcagcgtcgc ccaggccgga tccggcatca ggtctttatt gacatggtcc ctcattttat
    36541 aagcggccat cagggcccag tcctttttcc cctccatggt ggcggtggct gcgaggaaat
    36601 gataattgtg cggataatag gccagcgggt aaacgccctg ggcatggcaa ctgcttacgt
    36661 agtggctgtc gatttttacg gcgttcaggt tggccagtga gccttcatgg taatccccgg
    36721 tgcggatata aatatgagag ggcatatgga ccaggtggcc ggcgcccggg accatgcccc
    36781 ggtcaaattt tcgcgcgctc tccaggcccc tgccaggatt gaatgatgct tccacggcat
    36841 ggatataaaa atgatgaaag ccgggatggt tgggatattg tttgagcagg cgttcgatga
    36901 tggccacgat ctccggcgtc cagggttttg gattaccttt cttatcccaa agatcccagg
    36961 gatgaaggtc catgaccgat tccacataca gggcggcgat ctccgaatca tcgggatagg
    37021 tttcatgcaa ttttttcatc gctgcggaat aggcgctgtc cagggctctc cggtcttcca
    37081 cagggtccgg tacgtagcga gttgccaggg cctggatcat ggctttttct ttagggcttg
    37141 ccggtccgga atattttata gcctgttgaa cggctgcata agcccgttga taattgtctg
    37201 gttccatgcc tgcattgtaa ttgggaccca atacgtaagc gaagccccaa tggcacatgg
    37261 cacaggcaga atccagtttc gtagcataat aaaaagaccg ggctgcttcg gcatggttaa
    37321 aggcatagga taatttcaat ccctggtcaa aatattgttg aacttgggca tgtgaggttg
    37381 tgatcggata atgcagatca cccaaacctt caaacagggg ggccacctgg tcagtggaat
    37441 accaggcggc gtcggtgggt tgtgcaccgc acatggatac cggcgcaacc ataccgccgg
    37501 aaggaaccgg tgtatccttc gatttacaat gaaacaaaat acaaaggcaa atgccataaa
    37561 aaatattttt catgtattgg aatttggggt tttgaaattt attccaaaaa taatcatttc
    37621 atcaggcagg tcttaaacga ttccgggggc ggctgatctt tatttgctcc aatcattccc
    37681 agattaatat ttaatttacc ggccactact cgttaatcca tttcatgcag ttcccgattc
    37741 tgatcattct atttgttttc cacgccataa acagcctgga caaccacaag ccgccaagcc
    37801 catttggcgc cctgcccagc gcaagacaac tggcctggca cgagaccgag gtctatgggc
    37861 tgattcattt tacacccaca accttcgaga ataaagaatg gggctttggc gatgccgatc
    37921 cctccctatt taacccccgg gattttgatg ccggccagat tatttccgcc gccaaagccg
    37981 gtggtttaaa aggtttgatc ctggtggcca aacaccacga tggcttctgc ctctggccga
    38041 ctaaaacgac cgaatacaat atttccaaaa gcccctggcg caacggtaag ggagatatgg
    38101 tgcgcgagtt tgagcaggcc tgcagaaagg ccgggctgaa attcggagtg tattgttccc
    38161 cctgggaccg caacaacgaa tattatggcc agccagaata cctggatatc tatcgagccc
    38221 agctccgcga attgtatact gaatatgggc ccctgtttat gagctggcat gatggagcca
    38281 atgggggcga tggctattac ggcggaaagc gggaaataag aaaaattgac aatacggttt
    38341 attatgactg ggaaaacacc tggaataaca tcacgcggaa acttcaaccc atggctgcta
    38401 ttttcagcga tatcggacca gacgtccgat gggtaggcaa tgaaaagggg attgctgccg
    38461 aaaaacactg ggcgaccttc aaccctgaag ccgagaccgg gtctattgct gtacccggaa
    38521 atgtaaatac ccggatgagt aatgaaggaa cccatcgggg aaaatattgg atacccgctg
    38581 aatgtgatgt ccctttgcgg ccgggatggt tttatcatcc tgaacaggac ggcaaggaaa
    38641 aatctacgga ccaggtattt gacctttatt taaagagcgt gggccggggc gcctgcctgg
    38701 atctcgggct ggcacctggg ccaaatgggc tgctgcatga gaatgatgtc cgcatcctaa
    38761 agcaattcgg tgaaaaagtc cgggctacgt ttgaaaccaa tctcatccgc ggagccaccc
    38821 tcatcccatc ccatactaga ggcaaggctt atgcggctaa aaatatgcag gacggtgacc
    38881 gctatactta ttggtcaact tcagacaagg ttcagaaagc aaaaattgaa attgttttag
    38941 ctgaaaaaaa ggaattggac ctgatccagc tgcgggagaa tatcaagctg ggacaacggg
    39001 ttgaaagggc cgatattgaa atcatggaac agggtggttg gagggccctg gtatcagtac
    39061 caaccatcgg ggcaaaccgg ctgattcgcc tggacaaccc aataattaca gaccggctcc
    39121 ggattacgct cgaagcgccg gcgcccctgt gcataagtga aatcggcttg tacaaaatcc
    39181 aatagcctgg gcgtccggtg tattccatag tcattaacca atgatcccat acagccaatg
    39241 gtatccggat agtattttta ctttatgatc agaaggtctt gtattctatt gaacctgtgc
    39301 ttatgccttc ctttcttttt accgggtcag aacatgtccc tggaaaccct ccaggataaa
    39361 attaaaggag gttgggcagg acaggccatc ggggtcacct tcggcgggcc ggtcgaattc
    39421 cgcttcaacg gcaccatcat ccagtcctat cagccccttc cttggtacag gggttatgtg
    39481 gcggaaatga tgaaatcaaa cgaaggcctc tacgatgatc tttaccttga cctcacctgg
    39541 gtcgaagtgc tggaaaaata tggcttgcac gccccggcag aacattttgc taaggctttt
    39601 gcttacaaag ggtacaagtt atggcatgcc aaccaggccg cccggtacaa tatccttcat
    39661 ggaatagcgg ctccgcacag cggccattgg atgaacaatc cccatgctga ttgcatcgat
    39721 ttccagatcg aagcggattt tgcaggcctg atgtcccccg gcatgtccca gtccgcagcc
    39781 ctgatctgcg accgggtagg gcatatcatg aactacgggg acggttggta tggtggcgtc
    39841 ttcattgctt ccatgtattc ctgggcattt atttcatcca atatccctaa tatcgtatcc
    39901 caggccttaa aatccattcc ggccaacacc cggtatcacc aggtgattgc cgacgtgctg
    39961 gcctggtaca aaaaataccc gaaagactgg accaaaacct ggctggaagt acagaacaaa
    40021 tggtcagagg atatcggttg cccggatggg gtttatgccc cttttaacat tgacgctgcc
    40081 atcaattcag cttatgtagt tattggatta ttgtatggga aaggtgattt cacccagacc
    40141 atcgagatca ccacccgttg cgggcaggat gcggattgca atccggcttc cgcaggagga
    40201 atcctgggta ccattctggg ttaccagcaa atcccggctt attggctcca gggcctggaa
    40261 ggggctgaag atctcaaatt tgcccaaacc gattattccc tgaacaaagc ctatgaagta
    40321 agcctgaaac atgccatcga gcatatccgg ctcaacggag gctgggtcga ggaaggaacg
    40381 gtgcgcatcg tggcgcaaaa accggtcccg gtgaaatttg agcagtcttt tgaaaatacc
    40441 tatccagtcc aaaaaaccta tatcgggacg gtccttgaga acgagtatta ctttcctttt
    40501 gagggcaccg gcattgtggt caagggttat gtcagcaatt gggacggcct tggcccggcc
    40561 tattccgcca aagcagaaat ctacctggac gagattaaaa ccgaaaccgt ggatctgcct
    40621 tccaatttcc ttacccgcag gcatgaactg ttctggaaat atggcctgcc caaaggacgg
    40681 cataccctca ggataaaatt gctcaaccgg gataaacaat acgaggtacg gatcaccgac
    40741 atcctgactt atagcaatac ccgcacccaa atggaaaaag tccaccattt tggtcaaaca
    40801 ccaaaaaaaa attaataatt aataattaaa gaattaagga ggatctaccc ttaattcctt
    40861 aaatctttaa tttttttaat tgcattatgc atattcccag ccattttaaa atcgaggacc
    40921 ccctgatcat cgatcagttt attcaagacc atggattcgc cactttgatc tcccaagggt
    40981 ccccctaccc cctggcgaca catatccccc ttgaactgga aaccctgcct tccggcattc
    41041 aaatcctcca tgggcatatg tccaaatcca atccccaatg gacctatttt gcaggtcatc
    41101 cttttgtgct cgccgtattt atgtccccgg tgcatcagta catctcgtcc tcctggtacc
    41161 gccatgccaa tgtatccacc tggaactacc tgagcgtcca ggtatcgggg agaatccggc
    41221 tgatcgaagg ggaaaagctt aaagaagcgc tccgccgtct cacccataaa tatgaaaaac
    41281 attccgagca gccgcttgaa tttgatcagt tgccgccgtc ggtgcagaat cagatgcggg
    41341 gggtagcggg ttttgaaatc gagatcgaaa aaaaggaggc ctccttcaaa ctcagtcaga
    41401 accggaacga agaggatttt gccaacatca tccgtgaatt acgcaaaaaa ggaaacccgg
    41461 aagccgataa aatggcggag ctgatgatcg ctgccgccca atccaaatca aatcatgtct
    41521 gaatttcccc gccttttcct ttattcgctt caattaaatg aagcccatca ccggtctttc
    41581 tcagaactgg tcggaaagca tgcaaacaat ataagcatcg gcctgatcga aaatgcggcc
    41641 gatgtcatcc ccgattccgc aggctgggtg cgggatatcc gtgagcgcct aaggaacgag
    41701 ggatatcaaa tagaagccat agatctcagg gaatggaacg gtaaaccgga agccctccgg
    41761 aatctgatgt tttcaaaaga cgtgatctgg accggcgggg gccataccta ttacctgaac
    41821 tggattttga agtcatccgg ggccgacggg gtcattcggg attttgtgca ggcaggccgt
    41881 gtgtatgccg gctggagtgc cggggctgca gtggccgggc caaccacccg tttttacgat
    41941 gccatgggcg atgatccttc ggctgcccct gaacagtcgt gggatgggct gggcctgacg
    42001 gaaatatcca tcgtgcccca tatcaatcat cccgatttca gagggggggc gctggaaacg
    42061 gagacccttc taaaaacggc gggatatata cccctggcat tggaagatga ccaagtcttg
    42121 atcatccagg gagaaaagaa atgggtggtt taatacccta ttctcatttc acggaaaaac
    42181 cataggaggt gcttgacctg aaggtctctc caggctggag gatggtattg gggaaggccg
    42241 gttggttggg ggaatccgga aaatgctggg tctccaggca aaagccggcg tgtttttgat
    42301 agttcctccc ctctttggta tccgtatgcc cgccccttaa aaagttgccc gtataaaact
    42361 gaactcctgg ttctgaagta tgcacggtca tcaaacgacc gctccccggg tgataagccg
    42421 tcgcggcaag ggtgccgggt ttattcaata caaagttatg gtcgtaacct cctttgatct
    42481 gactgatatc cctgcctatg ggtttggctt ctgtaaaatc catgggcgtg cctttgacga
    42541 gggccagatt gcccaggggg atgaggcttt catccaccgg ggtgaattgg tccgcgttga
    42601 tatacaattc atgtcccagg atggtgctgt ccgtgcccgc agagaggtta aaataagcat
    42661 ggttggacag gttgaccggt gtggcctggt ccgtcgtggc catataatcg attttcaatt
    42721 gattgtccca ggtcagcgta tacgtcactt ccacattaag atttcctgga aatccttcct
    42781 ccccatccac gctcaggtag ccaagagtga cgctgctgtc ggtatggctc aaagccttcc
    42841 agagggcctt gtcaaaaccg cggattcccc cgtgcaggct gtttttgccg ttgttggccg
    42901 ccagcttgta ggttttgcca ttcagggaaa aactggcttt ggcgatacgg ttggcatacc
    42961 ttccgacaat gcagccgaaa aaaggattgc cggcttgcct gaacccttcc agggtattaa
    43021 agcccagcag gacgtcgccg ggaacgccgg tgcggtccgc gaccatcagt tgggtaatgg
    43081 tggcgccaaa attggtgaca gagacttcca tcccattgtt gttcttaaac gtaaagagcg
    43141 aaaccgtatc ggaatcgatc atgccaaaag gagcgatcgt gatcatttga tgagtgcttg
    43201 gcggcatggg cgccggcgtt tctttttgtt ctccggactt gcaggcagcg aatgccccaa
    43261 gaattaaaat cccggcggag gagttaaaaa tggttttcat acactctaag attcctgcaa
    43321 ataaagatat gaagataaaa aagggtttgg cccacagggt aaatattttt tggagccggt
    43381 ccgaattatc agatggactt gcattttcct tatacatttg acctcattaa gtcatgcaac
    43441 acgacccatt cgcagcgctg cggataagag agttcaggtt ttttatttta ggaagattat
    43501 tgtttatcac cggcttgcgc atgatggcga ccgtggtggc ctggtggctg tatgagacaa
    43561 cgcgcgatcc tttcgccatc ggtttgatcg gactagcgga agtgatcccg gcggttggat
    43621 tggctcttta tgccggccat ttcctggata cccacgaaag gtccgccgta ctgaagaagg
    43681 cggtgatgct ctacggagtg gtatccttcc tgatgtttat cctttccacc tccctggtcc
    43741 aggcgcacac ggtacaaaaa acctggatag ccggagtgta tctcctcatc tttttcaccg
    43801 gcgccatccg ggcttttgcc ggctcttcct tccagtccct cctggggcaa ctggtccctt
    43861 ttgcccttct tcccaatgcc attacctgga gcagcgggac ctggctgatt tcgtccatta
    43921 tcgggcatgc ggcaggcgga ttcctgattg ccctgattgc cgtaaaaggc gccctgctga
    43981 tttcattcct gtgctttgtg gtcgcctgcc tagtgctttg gaagattgcc cccaaacccc
    44041 ttcccgaatc ccgtcgccac gcttccacct ggagcagtgt aaaagagggc ttgtcctttg
    44101 tgtacaaaac caaagacctg atggcttcca tgaccctgga cctcttcgcg gtcctctttg
    44161 ggggggcggt agccatgatc ccggcctttg ccaaagacat cctgaatgtg gggcccctgg
    44221 cttttggctg gctcaatgca gccaccgata tcggttccat tctcacggtc acgacgctca
    44281 ccctttcccc gcttaaacgc aaacagggaa aaattttgat gatcgcggtt gccggtttcg
    44341 gcatttccat catcgtgttt ggtttatcca gatcctacct gctttcgttc atagcgctgt
    44401 tcctgagcgg tatgctggat gggatcagcg ccatcgtccg cgggaccatt gcccagatca
    44461 aaacgcccaa cgagcttaag ggccgtgtca tgtccgtcaa ttcgatgttt gtcaattctt
    44521 ccaatgaatt cggacagttt gaaagcggcc tcgctgcccg cctgatgggt cttgttccct
    44581 ctgtgatctt cggaggcagc atgaccttgc tcgtggtgat cattacctgg tttaaggcgc
    44641 cttctctaag gaaactggaa tattgacaac ccagcccctg ccatcctttc aacagccttt
    44701 tttatcttta ccgaaaaccc ggtttgccac tcctggaccg gtccgggcag atacaaagca
    44761 ttcaccgata tattcataaa gcaaatgatt cataccgagg cgagcatcgt gatcaaacgt
    44821 cccatcgacg aagtttttag attgcagaca gacctgggcc ggtacaaaga ctggcaagac
    44881 gggctggtcg aagcagcctg gacttcaccg ggcccggcgg gcgtaggatc gacatacaaa
    44941 ttcatcaccg cgaatgcagg catgcgtttc gaacttccgg gggaaattat tgaatgggac
    45001 ctcccgagag gctggaaatg gaaagcaagg agtggtccca taaccattga gggtggttac
    45061 cgctttgaac caacgggaca gggtacaaaa ttgaccatgt tctccgactc cgaattaaaa
    45121 ggaatgatga aacttatgcc ggcctggttg tataaatacc ttggggaacg ctcacaaaaa
    45181 cgttcattaa accagttgaa atccctgatt gaatccggta accagcatat ccaggaagcc
    45241 tcaacctgat tactgactac tgattactga ctactgacta ctgattactc aacaattcat
    45301 ccaccgccct gaacacatat cccctttctt tcaaaaggac gagcaaacgg ggcaaaaggg
    45361 aatagaactt ttccggtctg gccggtcctg ccccgatatg cataagtaaa ataaatccgt
    45421 tgagcccgcg ggccttttca aaccggacta cggactcata gatcgtctca ttgctccggt
    45481 aatttttatc ggccagtgtg gtataatccg tatgactcag cgtaccttga gaaaaattga
    45541 tcagttgaat gcctttctcg gcggaccagg ccgagatggt gtcgttatac cattcgaagg
    45601 gaggaagata atacaaggcc ctgcttacat ccgccccggc ttcttccatg gctttgtagt
    45661 ttaagtccag gtcggttgaa aattcgtgtt tgtcgaccag caggctgtcc ctgcggaccc
    45721 aatcgcaata aagcaaatgc tgatccgaat gcgcgcccat ataatgcccg tcccggatca
    45781 gtctgcgcaa atctttccgg aaaagcgggt tgcggtaaaa ccggccggtg aaaaaaaagg
    45841 aggccggcac cttattcctt ttaagttgtt tccggacata ggtcaaacct tcgccgaatt
    45901 catcacccga aaaaatcagg gcgatttccg ggaccgcggg atggccccgg gtaatggctc
    45961 cctggacata ggttttgttc tgatattgac ccgtttgtcc ggtcaggatt tgaggaccgg
    46021 ccccgatact taatatcaag aggggaagat attttacagc cgcaggcatg gcgcttattt
    46081 gatcaggcga agggcttcct ttttactcag tccggacaag gcatcgtgtt ctttcacaaa
    46141 ttcaaggacg gcctgaggat tgacccttga atattgcctc aaggcccatc caatggcctt
    46201 cctcaaaaaa aattcctttg aatgaatatg aggcaatatg gtctttttca tcagttcaaa
    46261 atccgtattc cccttatatc caagttggtg aagaatggcg ctgcggttga gccaaaggtc
    46321 cggatgacgg gtccaacgac ggatccgggc ggctttcttc tccgggaaca ccgaaaacca
    46381 gtggcctgcc gccttagcgg ccaggaggtc aaccgtatcc caccaggatt tgcggaggat
    46441 cagtttttca ataaggtcta tggtccctgt cgtgtaaacc ttccgggcgg cccagagaat
    46501 ttccaaaccg cagtattgca tctcccgctg atcgaaggcc caggtttgaa gaaggatggt
    46561 ttcgagatgg ccttttttgg caaatgcgag gaggggattc cggtagggtt tgaagaccag
    46621 gtttcgttcg gtggaagaaa ttccgaaata ttgaaaccgg tttttcatat accgggccat
    46681 ggccgacgcc cgttcagggt cggccttgtt ttgcatgtcc ctgtagcatg ttttaaggca
    46741 ggattcggga gtcccgcagg aagccgggga gtctaattga ggtgaacttt gggccaaaga
    46801 ctgacggcat tattgaggat aaacaacccg gtgatctcat aggtaaaata atccctgatc
    46861 ggaaagtttt ccaccaggtc ggcaacttgt tcccggtttt cggcgtggaa aacgatccag
    46921 ccgctggaac ggtcctcact gatcgcatac atttcgatga cgccttcgat gaggaggtcg
    46981 ttgacgatct tccggtgggc aggcaacaat tcccagaatt cctcgtccag tttgtccaga
    47041 tgcacattga ccatgtaact ctccatacga ataaaattaa aggaattagc cagaacttcg
    47101 tgaattaaat aattaaagga atccctcttt tggaaaagcc tgcatgccct gtttccctcc
    47161 ccggtgggtt taaaagtctg aacccaggga tatgtaaaac cgcggcttta gcacttcacg
    47221 ggtttcaatg ccccaggcat aatcggccct tacctggtaa cccagcatgt tcagcctcaa
    47281 gcctgtacca taaccgataa ccagcggatc cctggcgtaa accaggctga tactgacctt
    47341 gggattggat acggaagtgg tgttgagcgg gctgtcctga tcaaaggggt tccgcccaaa
    47401 ccaagccgtt ccggcatcca cgaaaccgac cacctggaaa ttccggagaa agaccgaacg
    47461 taattcccgc ctggacaagt attggattaa tgggattctc agttccgtat tgaacaatac
    47521 atggctggat ccgttccgga tattttgttt gaatcccctc aggtgagccc cgagtgcata
    47581 ataggcataa ttgttgtctc cgggtattgg ggtatcgtcg tcgtattggg gggtgagcca
    47641 attgtcgacc ccgcccagga aaaacaaaat acgctctgag ccataagagg ttgccccgga
    47701 aaggcgcatg gccaagatcg tcctgcggtc caagccgata taatgacggg catcaaaacc
    47761 cagaacggtc ataaatcctt tggccaggtt gagtttccag tcatccacga tctggatctg
    47821 cattttttta accagttcgc cataaaactt ggcacgcgta cctgtaaaac ggttgatatc
    47881 aatgggcgtg gtgttgtcat agacatattc tgcccggagg ccaaaccgct gattggtttc
    47941 agggtcggcg ggcaacgtgg tgggatctgt ggcaagcaaa gtgctccggt caaaccggaa
    48001 ggtggcggcg agccgggcgc tttgataagt atcaaaggga tatttcaatt ggtagacccc
    48061 gatcacgctg gaattgcgta ccctttggga aaggatctgg ctgatttcca ccgtggaata
    48121 cctggactgc cggtagatgg cgtatcgttt gtcaatacgt cttttattat catcgaaaat
    48181 caggaaatat tccgtgccgt tgaaggtggt gggtatccgg acccctcctt caaagctgta
    48241 atcttcaaac aaatctttgg tctgggcttt gaccagaaag cccgtggggg ggtactcaaa
    48301 tccccttttc tgacccgcat agctgttgag gccgccaaac aacaggccat tgtccatggt
    48361 ggtcacgaca ttatccagtt tgaatttcaa cctggaggcg accatcctcg taaattgaaa
    48421 cggctccgga atctcggtcc tctgtggttt aaaaacagcg gaatcccgga ccggggaact
    48481 taccgatggt tttttttccg aatccattgc aggcctgagg ggggcttggg tcatgccggg
    48541 aggatcggga aattcgcttt ggaataaagc gatcgttttg ggggtactgg tcacttcccc
    48601 cgtgatccgg cccgtggaaa gaccctgagg agccattcct ttgaggtcca ttcgattttt
    48661 aatatagacg gagtaaccgg gagccagggt atccgtgacc cggatggctt ccgtaaatgc
    48721 caggaccttt cgaccgtttt tgatcactcc taccgcactc tggtccgcac catggaaggt
    48781 gataaaatcg tggtccccac gggaagaatg taccaggctg ccggaccagt ccgcatcaat
    48841 aaccatggtt ttacgctggg tcacgccatg gtcattaaaa agaaaggaaa cctggttttc
    48901 accggaaaga cagggtttgt ccagttcaaa atgaatattg gaagtcagcc gacggagctt
    48961 tttggactca atgtttttgc ccaggtcgaa gggcatgaaa aaaatattga aaggctgaat
    49021 cggaaggagg gtgtccaccg gcatggtaaa taaggtgtcc agggcacggt tggatttaaa
    49081 caggaccccc ttctctccgt tgagcgaaat ataactggcg tccaggtcat cgtaaaaatc
    49141 ctgggtcagc ctgcgacttt cccgggtcag gggaaggtaa tagtacaagt ctgacaaagc
    49201 atccgtggac gcattgagca aaagggtttt atcatcgatc cagtcaatcc cgtacacccg
    49261 ctggtattcc ggggacaatt tatcctggct ggatttgccg gtcctgagat cgattcggta
    49321 caacatccat acgtcgtgtt tctcggtgac caggaccatt tcatttccat tcggatgcca
    49381 ggcaaatacc gggatctggt aatcggtttc ctgtacattg tttttgaccc ctccccggaa
    49441 tatcctcgcc ggtttgccgg acctgcggtc ctggacaaaa accttgtacc ggccgcgatc
    49501 gttgaccgca taagcaaacc ggttgccgtc cggggacacc cggaccgcgg acaccggcag
    49561 tttcttttta tttttaatcg tcagcgcctg gttttcattt actgcccgga aagcgcttcg
    49621 gtcctcctgg taatttttcc tgaaataact gaggctggcg gccgctattt cttcaaatgg
    49681 cgtgcccagg atatacagga aagaagattt aatgttccgg tttatccggg ccaggtagag
    49741 gatattggag acattggatc tcccgtattc gcgggtgaga taattccaga atgcataacc
    49801 catcagcctg gggtgatctt tggccaaggt ttcgaatttg cggttggggg gactgtgttg
    49861 caggaggtcc ctgaattggt tgtcggcccg ggtattccag tcttctccca ggtaagcgat
    49921 caggccctcc ctgaaccagt ccggaatttt taactggatg gatttctgca caaaaccctg
    49981 caggccgctg ccatagatca tggcattgag gtatacctct cctatgccct cccggatctg
    50041 cctgcgcagg tgattgtggt ccccgtcaaa atagacaaag acccggttgc ccagtaccgt
    50101 ggtccggtca ttccggctga taaaaacttc atcgataccg atattggatt gtttcaggtc
    50161 actgatatcg gtataacaga tgatttcaat tttgtcattc agcttatgct ccaggatgct
    50221 ctcaatgctt ctaaaatcgt attccgccag ggatagggtg atctggccca cattcctcgc
    50281 ctttccatac cagtaattga caaaattctg ggtctcatac atgtaccagt catcaaagtc
    50341 atcgtgatgc tggatcctgt tctttccgaa ttccgtctcg ataccgcttt gggcatagct
    50401 ccaaaatgcc ccaaaaatga atccaatgga ccaaatcagc ctccaagcca tggaataaag
    50461 ataaaggaaa atggatttag gggttaaggt taaggctaag gctaaggtta aggggacacc
    50521 atccaccaac tctaaaatat cttcaccatc ccctcgtacc tcaccttcca cttcccatta
    50581 tcagggaatc ccggggcctt accgcccgga tctgattccg tgcctgcgca catcatggct
    50641 atggcggcaa gcaggcctcc gttgccggga aggtaaaggg tcagtcttgt gtcctggtaa
    50701 ttgtggccgt tgatgagata ggtgtttttt tgcacaggca tgagcagggc ttcgacggcc
    50761 gcttccggcc ggttcaggcg gcaggcggcc atggccatca tcgggtaatc ccatccccag
    50821 gtagtctgcc agttccattt ttcaagaatg gtattgaggg tatgattcat aatggaagta
    50881 tccaggccgt tggaaggcgg tatgccggct gaggctccca ggacggaagg gtggtcacgg
    50941 aggtattggg ggttggtata tgagtctggt gcagaccggg cggccaggta taccccctcc
    51001 cggtgaggca agggcgcgag cctttctatt ttgtctttcc attccggatg ttccggcagc
    51061 cccaggcgct tacgccatcg ctgggccacc gaaagggccc aatgccaata ggccaattca
    51121 aaagtcggat tgacggtggt gagcggatcg tggctttcct gggcagggat caagccgggt
    51181 ccaagattat aggtatgact gctgctgtcc caggccagga aatcagccat aaagtccgcc
    51241 gtggcaaaaa caagctcttt atacttattc agttttttcc gggaaggctg gattttgtat
    51301 aacaactcac tcatgtaaat gacatgggct tgctgccaga ccaggaaaga tccgatcgag
    51361 gatggggtct cgtttcccca gggatcggtc attttctgcc agcgaacccc tttataacct
    51421 tgccggagtg ccagtttttc cgcattcggc aaggctttga aataccaatc caggcttttc
    51481 ttcatcaatt cgggcctccc ccaaagcgca aagtggacgg catgccacca atgcatttcc
    51541 atatgaggac gtccaaacca actgttatag gtcagcccgg tctcctgcgg cggggtcgga
    51601 gacaccccct gtacccgggt aaggtactgg gaaaggacga tccttcgttc cagctcatca
    51661 gccctttgat ccaggctgcc ggacaggtcc agggctcccc cgctcatcca gaattttttc
    51721 caggcctggg cgctggccaa acgggttttc tcaaagtcag gaaccttcct tttggaggac
    51781 tgctctgaga acaatacagt caattcaaat gggcccttag tcccgggttt tattacaaaa
    51841 tattgaggtt gcacttcata aatatccgct gccgcattcc attgcatttg gatcaaatag
    51901 tggctgcggt caattgatgc atagatttct gcgccccctt tgtgtcttgc gagtctagat
    51961 tgttgggttg aatcgtaggt ccagttgatg ccggtatcct tccaaaggcc ggtgggggag
    52021 ggaatcctga tccgtatccc gatttggttc gattccagca agggtccttc cagccggaag
    52081 gacacggcgt ccattttcgg atgggctgcc gtgatcaccc tgaccagccg ctggtccaag
    52141 gcaaagctgc tggtcaccgt tccggtccac aaatccattt gttgatggat ctgcccgaga
    52201 tcggccaccc gaaccaacct tccatcgtca tgctgcattt ccaggccgac attggcgagg
    52261 tggagtcggt gcggattgga cctgaaataa tcggtggctt cctgtttcct tttagcctca
    52321 tggtcctgga cagcgtatgc ggttttcttg ccattaaaga catactccct cagggtttcc
    52381 tcaaaagtat agcccccctt attgggaaat gaatgccagg cccagtcggt ttgcgtcccc
    52441 aggggtatgc cgcccgcata ggcttccgga aaagtctgca tgccggtggc atccagggta
    52501 tatgcgaagg ccccattgcc gacagtcagg gctgacaagc tgtcgaatcg ggtgatggaa
    52561 atccggtgcc ggttgacgac tgcttttcgg tcgattgggg cctgggcgga aaccaggttt
    52621 gaggaaataa ataaaatcca ccagcaacaa taggtataaa atcgttgttt tcccttccac
    52681 atcggaatcc tcaagataag gtccgaaccg gaaagttcaa gaagtaattt gaaatcaatg
    52741 aattatcctg ccacccattg cctggttcat cctcagccac ttactatcct gttactggcc
    52801 ttaatagatt tttaacactc cccacttaca atttttgttg aagcaataac ctatatttgt
    52861 aaatcttgaa taaactgagg tatatattga tcttcaccct ggtgcttgcc ttgtcaccct
    52921 gcagggacct tatgatcctg taccttccca ataatatcgt attgaatctt cagccgctca
    52981 ttgaagaaga aaaccacagc gatacccagg aaaaatccgg taaagaaaag caagacaaat
    53041 attttggcct ttatatggcc tccatccggg aatccgattc ccgcgcctcc atgctgatcg
    53101 tcgatcagaa gattgctttc gaaaaccact gcgactccct tacccagcct cccaacgcct
    53161 gacattgaat agtcattccc ttcgcggaac caggcactcc tgtgcccggc tgtttcttat
    53221 ttattctttt ctaaaaactt ctattcaatg cgtactcata aagacatcct tcggacagag
    53281 atgaccccgc cgcaggcact ggaaattctc aaagaaggca accttcgttt tttaaataat
    53341 atccggatca accgggacct gttaaaactg gtggacgaac ataccgaaag ccaggccccg
    53401 tttgcggcga tattgggttg cagtgattcg agggtacccg ttgaaattgt gttcgaccag
    53461 ggattgggtg acctctttgt ggtccggttg gcgggcaata tcgccagcat caatgccatc
    53521 ggcagcctgg aattttcctg caaactgctg ggctccaaaa tcatcgtggt gcttgggcat
    53581 acgtcctgcg gggctgttaa agcggcctgc gacaagacct acctgggcaa cctggagacg
    53641 ctggtgaaaa gcattgaacc tgctgtacac gatgaaagtg tggcgcatct caaaagggat
    53701 tccaacgaca aggattttct caaaaaagtc accatactga atatccggca ccagatcaat
    53761 accatttacc acgagagcga gattctccgg gagatggtgg aaaagggcca gatcattctg
    53821 gtcggggctt tgtatgatgt gaattcagga gaggtccagt tcttagatcc gatggattaa
    53881 ccggaaggga ggtatggaat agaattaagg taccgatttc catttcagaa atatccaaaa
    53941 aacgcaaccc aatgaatcgc atttccataa aactcagtaa aggggacctc tttgggggtc
    54001 ttactgcggg tattgtggct ttgccgttgg cactggcttt tggagtccaa tctggaatgg
    54061 gggccaccgc tggcctttat ggtgccgttt ttcttggatt ttttgccgcc cttttagggg
    54121 gcactcccca gcagatcagc ggaccaacag ggcctatgac ggtggtgacc gcggcaaccg
    54181 tagccgcatt gattgcaaag gccggggact tcaccacggc cttccccgcg atcattgcca
    54241 cctttgtttt ggctggtttg atgcagatcg ttgtggggac cctgcggctg ggatcctata
    54301 tccggtacat tccctacccg gtgatttccg gttttatgag cgggatcgga gtgatcatca
    54361 tcctgtttca gttttttcct gccttgggcc tcaacagtcc ggcgaccacg cttgaagttt
    54421 ttggagcaat acccggcgcc ctggcgcaag tcaatatctg ggcattgatt tttaccctgc
    54481 tgaccatcgg gatcatttat ctgtttccta aactgacgcg atcggttcct tccaccctcg
    54541 tggctttgtt ggccgtgacg gggctgtccc tcgtattggg ccacgaaatc ccggttatcg
    54601 ggaacatccc gcagggtttt cctcccattc acctggataa gatcttctcc gttgatccat
    54661 ccctgtttat ggtgatcctg gaagccgcgg ccacgctggc ggcgctgggt gcggtggatt
    54721 ccctgctgac ctcggtgatc gcggataacg tgaccaaaac gaaacattcc agcaataagg
    54781 aattgatcgg ccagggagtg ggcaatgcgt tgagcggctt atttggaggg attccgggag
    54841 cgggtgctac catgcggacg cttgtcaatg tcaggtcagg cggcaaaacc aggctctccg
    54901 ggatgatcca tgccctcgtc ctcttgttta tcgccctcgg cctcggcacg tatgcggccc
    54961 tgatccccaa atcggttttg gcagggatcc tgattacagt gggtataagc atcctggact
    55021 accgggcgct tcgccagatc cggcaaactc cgctcacgga tgccctggtc atggtgatcg
    55081 tcctggtgat caccgtcttg tttgacctgc tgattgccgt cggggtgggt attttgctgg
    55141 cctcgatcct gtttatgaaa aaaatgggag acctgatgga agcgaattcc tatatcctta
    55201 gcctggaaaa agtaaaggct gaagaaaaaa agatcaatga gtccattcct gaatcgctta
    55261 aagattccgt gtttgtaaaa caaatggatg gtccgttgtt ttttggatca gcaaccggct
    55321 ttcaggacct gcttaaaaag atgcccgata cgcattttgt gatcatccgg atggataagg
    55381 tgccctacat cgaccagacc ggattttatg ccctggaaga ttccatactc gcgctggaac
    55441 aaagaaatat agaagtattg ctctgtggaa taaatgaaca gccgctgcag cggctgaagg
    55501 cggtaagact gattcccggc ctcatcagca aggaacatgt tttcccggat ttttcagcct
    55561 gcctggagtg gttatccgtc aacccggtat ccgataacag gcacttcacc gtgcctgcct
    55621 gagatactta ttgtacctga ttccgtccaa aaccattatt cagttcgggc ctgttcccgg
    55681 aagtccgggg cccgtgtatt caattaatat aaataataaa aaaatgaact tatccaatat
    55741 aaaagagaat cttaaatccg atctgctgtc cggatttgtt gtattcctgg tggcgctgcc
    55801 gctctgcctg ggaatcgcac tggcctcagg cgcgccattg gttgccggcc tgattgccgg
    55861 cattgtaggc ggcctggtaa tcggtccgat cagcaagtcc cagctcagtg tgagcgggcc
    55921 ggcagcgggg ctcacggcca tttgccttgc ggggatcaac agcctgggtt cctacgaatt
    55981 gtttctcctt gcggtcctcc taggaggagg catccaggtc ttgctggcct ttgtcagggc
    56041 cgggagcatt gcttattatt tcccgtccaa tgtgatcaaa ggcatgctca ccggcatcgg
    56101 gatcatcatt atactcaaac agttgcctta catgctggga tatcgtacgg atgccggaag
    56161 cgccaccgcc tcttcctcct cttttgacct gtttgattac attcatcccg gcgctgccct
    56221 catcggcctc atcagccttg cgatcttatt cgcctgggac aaatacaaac cggtaaaaat
    56281 gttgcccggg gcgctgttgg cggtggtttc ggccgtattg atcaaccagg tttttatttt
    56341 aaccggtgct ggctcaatgg ttgcgggaag cggtttcctg gtcaaccttc ccacctttga
    56401 taatttcagc agtctgacca gccaattgtc ctttcccgat tttggaggca tcaccaataa
    56461 ggaagtgtgg atcctggccc tcaccttggg catcgtggcc tccctggaat cgttattgac
    56521 catcgaagcc accgaccgat tggacccaaa taagcgggtg actcccacca accgtgaatt
    56581 gctggcccag ggaatgggaa atatcaccag cggtttaatc ggcggtttac ccctcactac
    56641 ggtgatcgtg aggtcgagcg ccaacatcaa cgcaggcgca aaatcaaaga tgtccaccat
    56701 cgtgcatggt tttctcctgt tgattggggc cttgtttttt gcaaggtacc tcaatcatat
    56761 cccccttgca gccctggccg ccatcctggt ggctaccgga tataaattaa gccaccccgt
    56821 ggttatcaag aaaatgtttg ataaatcaaa ataccaatgg cttccctttg tggtcaccgt
    56881 cgcggccgtg gtgctcacgg atctgctcac cggagtgatg atcggcatgg ccttcagcgt
    56941 actggccatt ctcataggca atatcaaaca cgcttacttc ttcaaagcag atgaatataa
    57001 atccggcaaa ctcatcaccc tggtgcttgc tgaagaagtc tctttcctga acaaggccgc
    57061 catacggttg accctttcaa aagttcccaa aggcagccgg gtggtcatcg atgcttccca
    57121 ttccacctat atcgaccacg acgtgttgga aatcatccgg gaattccagc acgataccgc
    57181 cccgactcaa ggcatttcac tcatcctgaa aggctttaaa gaggagtacg aaatcaaaaa
    57241 caacgatcat gtctatatcg gggagatccc ggaaccgctt cgaaagctgg aagccaaaaa
    57301 agaattcatc acgcagcggg aaaccctcaa tctcctgacc tgaaaaaaaa tttgtgagtt
    57361 ttttcaaagt ttgaggtgct ggcctccctt ttggtcagca cctttttgaa atcgcccttt
    57421 aatttttaat tcttaattct taattcttta atttcttaat tacttaaaca catgcgtatc
    57481 catacttatg aaaataaaga cacactgacc cctgaaaacg ccctcactta tttgaaggaa
    57541 ggcaacctcc gctttgtaaa caatatccgg gtaaaccgag atctcctgca attggtggat
    57601 cagtattcca ccgggcaatt cccctttgcg gccattttgg gctgcagcga cagccgcgta
    57661 ccggttgaac tgatttttga ccagggactc ggcgacctct ttgtcgtccg cctggccggt
    57721 aacatcgcca gcaccaacgc catcggcagc ctggaattct cctgcaaggt actgggctca
    57781 aaaatcattg tggtcttggg ccataccgaa tgcggggccg tcaaatcggc ttgcgactcc
    57841 gtggaactgg gtaacgtcac ttcggtcctc aaacacatag agccggccat cgctctggaa
    57901 aaagaaatcc aaaataaccg caattccaaa aacaaggaat ttgtcaaccg gattctgcac
    57961 cacaacctgg attaccagat cgaaacgatt ctcgatcaga gcgatatcct gagagaaatg
    58021 tttttccagc accagatcgc catcgtgggg gccttgtacg atatcaaaac cggtttggtg
    58081 agtttttatg ataatgcggc gcaaaaatac cgcgaaagag cattgatgag atccgcaatg
    58141 ggttaatgtt catttttttg attaaaaggt accggtctaa ggtttggccg gtgccttttt
    58201 ttattatatg aataatccgg cggaacaagc tcaggcctgt ttcatcagca attgaagcag
    58261 tttccggtcc agcttattgc cgtaccattc ttcatattgc acatcttccc gccggctgtt
    58321 gaggattcca aaatccccgg aaaattccca gagcgcaaaa ccgatctggt tctgcttcag
    58381 gatatccaat acatcgccaa accaggccag gaatacgggg tgcggggttt tgatccagca
    58441 gccgcattcc ccgcaatgta cgcccacccc ctggtttttc agatcgatcc agggtttata
    58501 atattcttcc agcatggccc tgctgaaata tttgtcccca acctgtccgg gccacagcag
    58561 ctcgggcatg ttttcccggt tattggagga ccaggacgcc ttgtaatggg agatcatgcc
    58621 cgggaaataa ccgcggcaac tctgggcgat ttttaaatcc gccagttcag ggaccaccag
    58681 gttgcccacg ttattgccat ccgcgatgat gaggtgcctt ttgttttctt tccaaatagc
    58741 ttcagaagca gctacagcga tctctcggta gaggagtccc ggcaccgcgc tccgggccga
    58801 gtgctgatca ttcatatctt cccgcacaca aggttcattc aacaggtcga aactgatttt
    58861 cttgcgggaa acgtctttaa attttttggc ccagaaattc caatgaaaat taaaggcttc
    58921 tactgcggct ttgtctttcc agaggttaaa aggttcgtga aagccggcgt taacacaaaa
    58981 gcccggcgcc cggtgcaggt tgaggctcac gtgcatgccg tatttatggg ccaggtgcac
    59041 cagttgttcg atgcggtcgg tagcctgggt gctgatctgg taggtttctt cggggctgat
    59101 gttccggctt cggtcgatat ccagataagc cgggtaagcc atcgggatgc gcacaaaatc
    59161 aaagccccag tcctgcatcc atttgaaatg ttcttctttc gtctgggggc gcgaggaaga
    59221 ggggtttgga ttaaaaaaat caagcaggtt aaagccccgc caggccggca gcttgttttt
    59281 gggtttgaga aaatcaagga actggccttg tcccggcagc tggaccgagg ccgcggcaag
    59341 ggccgagttt tggagaaatt cgcgacgaaa cataatataa atttaagtga agggatcact
    59401 taagtcaccc cctcacttca aaaaacctgt ttatctttcc atcctaaacc ggtaaacgtt
    59461 atgaaatcca tgctcctcgg cttcatcgcc acccaattgt ccctggcttc catttttgcc
    59521 cagtccggaa aagtctacga caacctttcg ttgcccagca agatcctcaa tatggaacgc
    59581 aagtacgcca tctacctgcc tcccgattat gaatcctctt cccgctccta tcccgtgctc
    59641 tatctcctcc acggcggagg ggacgaccag accggttggg tccagtttgg agaggtgctt
    59701 catattacgg ataaagctat tgccgaagga aaggccaccc cgatgatcat catcatgccc
    59761 gatgccaaca ccacccgcag aggctattcc aacaacgcct ccggcacctg gctctacgag
    59821 gattttttct tcaaggaact gatgcccttt gtggaaaaaa aataccgcat caagggcgaa
    59881 aaaaggtacc gcgccgttgc cggtctctcc atgggaggcg acggcagttt tacgtatgcc
    59941 cttcatcatc cggaattgtt ttcttcagcc tgtccgctga gcgcgggtac gggtcccctc
    60001 accctggatg ttgccaaaac caatgtgcgc cgtttcgatt ccacggccac cgacgcccag
    60061 gtcgaagcct tcttcaaccg gcaaagcgtg cccctcctgg tcaataatat gcccgacagc
    60121 ctgaagcggg ccgtacgttg gtacatcgac tgcggagatg acgacttcct gtatgaaggc
    60181 aattccctcg tccatatcgc catgcgtaaa aaagaaattc cgcatgaatt ccgcatccgc
    60241 aacggaggac atacctggac ctattggagg caggcattgc ccgaggtgct ggagtttgta
    60301 tcgcaggcgt ttcatcaata ttaatagtca gtagtcagta atcagtagtc agtaatcagt
    60361 agtcagtttc ttagccttag ccttagcctc aatcttagcc ttaaccttag cctcaatctt
    60421 agctcatgat tacctcaacc attaactatg aaggcgaact tcacactacc gccatacacc
    60481 tccggtcggg tgaagccatt gtgaccgatg ctccggtgga caacatgggc aagggcgccg
    60541 ccttttcccc taccgacctc ctggccactt cccttgcctg ctgcgctctg acgaccatgg
    60601 gcatcgtggc caataaaaac gggatcaatg tggacggggc aagcgcggaa atcaccaaac
    60661 acatggcttc ggacccgcga agggtgagca aaattgacct actgatcaaa atgccttccg
    60721 ataaaaccta tacccctgaa caaaaaaccc tgatggaata tacggccatc cattgccccg
    60781 tagctaaaag ccttcattcc gacctgctgc aaaacgtgct gtttcaatgg gggtaaacaa
    60841 aatcaggctt cactttccaa agccggtgat ccagggaaaa atccacctcg acggatccaa
    60901 gagcatcagc aaccgggtcc tgatcatgct cgccctcgca gaggacaaac cggccgggct
    60961 ggaacacttg tccacctcaa gagacagcca gcttatgcaa aacctgctcg gaagcaggga
    61021 ggaagtcctg gataccgggg ccgccggcac cacctaccgc ttcctcaccg cctacctcgc
    61081 cacccggggc cgcaaagtga tcctgaccgg cagcgaacgc atgaaacaaa gaccaatagg
    61141 tgaactggtc agcacgctca acgccatggg cgcaggcatt gagtatgtgg aaaaagaggg
    61201 ttttccccct ctccggttcc cgggaacccc cttaaaacaa attgcccgcc ggatctccat
    61261 ccggtccacc atcagcagcc aatacatctc ggccctactc atgatcgcgc cttgcctgcc
    61321 cctgggcctg gaactgcaat tggtcggtga ggtgatctcc cggccttata tcgagatgac
    61381 cctggccatg atggaccatt tcgggatccg cagcgcctgg gagaacgata ccattaccat
    61441 tgagccccag ccctatgtat caaaaccgct ttttgtggaa ggcgattggt ctgccgcctc
    61501 ctattattac agcctggcgg ccatcgccga ggaagccgaa ctggaccttt acggcatttc
    61561 gaaccacagc ctccagggtg acgcggccat cgcggagatc ggcaaactat tcaatatcca
    61621 aacgacatgg cacccggatc atgtaaacct gaaaaaatta cccgggcccc tgcctaaatc
    61681 ctttgaatgg gattttatca aatgtcccga cctggcccag accgtgatcg ccctttgcgc
    61741 agccaccggg gtgcacggag tatttaccgg gctgcaaacc ctgcgcatca aggaaacgga
    61801 caggatcgcg gccatgaaaa ccgagctcaa aaaattgcag gtcccttttt accggttgcc
    61861 ggccaaaatg tccaaggcaa gcaaaatcga atatcatatg gtggaaggca aagccgtctt
    61921 cgatcacacc ccggtctttg ccacttatga ggatcaccgg atggccatga gcttagctcc
    61981 cttatccctg atccatccca ttgaaattga agaaccggag gtagtggcaa agtcctatcc
    62041 cggtttctgg aaggatttgc agtcgctggg atgtgaaata tactaggatt cagcgatcag
    62101 cagtcagcaa tcagcagtca gcgatcagca gtcagcgatc agcagtcagc tttcagcagt
    62161 gcttgttgca ctacaacctt ttaagcttta gttttttgga taaaggtgcc aaa
//
LOCUS       DAOQXT010000015        60910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_522_length_60910_cov_7.922964, whole genome shotgun sequence.
ACCESSION   DAOQXT010000015 DAOQXT010000000
VERSION     DAOQXT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 60910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60910
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_522_length_60910_cov_7.922964"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..1264)
                     /locus_tag="PKM27_04050"
     CDS             complement(221..1264)
                     /locus_tag="PKM27_04050"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06463.1"
                     /translation="MIFRLQLKLHRLLHWEYWPMWTLYLSLAPWYLACSLRARSLGFF
                     NAVNPSFKYGGMSPESKDEMHLLFPPGMLPKSILIKKGEPLPDIEKRVDEQNLAFPMI
                     VKPDRGYRGKQVKLVRDLNELRQYAAGAGFDFLIQEYIPLPLEIGVFYMRYPDQSQGF
                     ISGIVEKKGVTITGDGIHTAGQLVGKSYRYHPQSSWLEKENKSVWNTILGKDEQILLS
                     PIGNHARGSTFYDSSHKASEKLNVVIDRLSQSIPGFYYGRYDIKFNSWEELENGLNFK
                     VIELNGAFSEPTHIYDPGHSYLFGLKEMARHWRHMAKIARLNHNLGHPYLPVTEIVPM
                     ISDIYQNKTDLDR"
     gene            complement(1261..1980)
                     /locus_tag="PKM27_04055"
     CDS             complement(1261..1980)
                     /locus_tag="PKM27_04055"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNR06464.1"
                     /translation="MCTVTYFHSGKTRILTSSRDEKTSCPPSAEPEMVLYGSRQLFFN
                     RDSRSGGTWFISDNQHRVAVLLNGAFRKHSPRPARRKSRGLVLIDLFKSEPFVPGFRA
                     YDLAEIEPFQVIYMDSHGLCRLVWDGEGKHIMGLDPYQPYIFSSVTLYDEAASQLRQN
                     WYSTFIKNHHRIQRQDLLNFHLHHQNEDPVNGLRILRENQYKTHNISQVEFSAEGALY
                     TYLDLAKQNPTEKWLSRAQPI"
     gene            complement(1968..2453)
                     /locus_tag="PKM27_04060"
     CDS             complement(1968..2453)
                     /locus_tag="PKM27_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06465.1"
                     /translation="MIQSIKEALTSLAQVLEESGQVIYIKPCPLLGNASIGMHTRHIL
                     EMFDCLDEGYYLGTISYDERKREGQIETDLSHALRLIQVIIHKIDRPNQDLHIKYSFH
                     GHPTTVSTNYYRELIYNLEHCIHHQALIRVALESMTGLALPEQFGVAPSTLHYRARCA
                     Q"
     gene            2732..5206
                     /locus_tag="PKM27_04065"
     CDS             2732..5206
                     /locus_tag="PKM27_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR06466.1"
                     /translation="MEKPNPVNARLELAQQFVQYTNRNIFLTGKAGTGKTTFLKNLKH
                     HTPKRIVVVAPTGVAAINAGGVTIHSFFQLPFGPFIPGQDRQGDSREMKFSREKIRTI
                     RAMDLLVIDEISMVRADLLDGMDRVLRRFRNPDKPFGGVQVLMIGDLHQLAPVIREEE
                     WELLRPHYPSVYFFESQALKLSRPLTLELTHIYRQEDEKFIGLLNRIRQKKMNPQDLE
                     WLNSRYRPEFEPTGEDGYITLTTHNATAQSINQNRLMVLKGTMQVFHALVEGEFPEST
                     YPNEFRLELKKGAQVMFIKNDTSREKNYYNGKIGTITSIREEEIRVLCQGEEEEIRVG
                     RVSWQNIKYVLDENKQLVEQILGTFTQFPLKLAWAITIHKSQGLTFEKAVIDAQASFA
                     HGQVYVALSRCKTFEGLILSTPLSNQSIKTDQAISHFSEEASQQNLDASTLYDSRKQT
                     QEEWIRDLFDFQALKKNLAFLSRLTEENKNHLVESAPVAVQELKNRLNAEVLVVVEKF
                     LLQLPYYLEQAVLPEENQGLQDRIQKGCSYLLPKLEGPWMDTLQQLDLDCDNKEMKKK
                     LRQALDQVLQEARVKMEVLKACRAGFESTHYLQAKTNAGLEPEIKKSSPKGSKEESTS
                     PEQPGLYQVLKQWRDGLAETNKLPVYMIMPQKAMKQISALLPGNLAALASIKGIGAQR
                     LAQHGEQILHLIEEYCNRHQLSPHCLEETNLPPQKKIQKGETQQRTYQLFTQGKSIPE
                     IAQERGLAVSTIENHLAKYIAMGLLRVEQIMAPDRIGRLKTYMEDHSHAGNKEIKDHF
                     GEEFSYGEIHMVRESMTGPGQNNIPV"
     gene            5753..5944
                     /locus_tag="PKM27_04070"
     CDS             5753..5944
                     /locus_tag="PKM27_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06467.1"
                     /translation="MAHFEELRFNEKMEVDGGWVVTFSRAVLKKTIVGSIALFVIDNW
                     STTKEAVLDGVQDGMKTWN"
     gene            5957..6094
                     /locus_tag="PKM27_04075"
     CDS             5957..6094
                     /locus_tag="PKM27_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06468.1"
                     /translation="MKDKINKLLEYLDQLHPFIYIFFVIGFLYVLKFVIKELYSLFFN
                     H"
     gene            6107..6283
                     /locus_tag="PKM27_04080"
     CDS             6107..6283
                     /locus_tag="PKM27_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06469.1"
                     /translation="MIELTFNETTQITGGAPTRKTSFVYDFLYAISYGVTWFLGQGDE
                     MSEAIVRQDLKHVW"
     gene            6544..7629
                     /locus_tag="PKM27_04085"
     CDS             6544..7629
                     /locus_tag="PKM27_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNR06470.1"
                     /translation="MLGLFLGLVFLFIGFAPVPVNLKLQGMIRPSSERAELVCLTAGF
                     IDSIYVKEGEAVDAFQPIVSIINKERATKMDQLLTEIGQCLSDLRDLDLLCKVTGAVS
                     PEQISNPAFKQELLKYGFEVKKLELSLAKSENEFKITKELFMNKIIAPKEYSDKEFEY
                     LHHLAEAETVKRQQMILWQQARQTKGRELNALQSQLKLYQDNETWDMVRAPVSGIVLG
                     TRSIYKGSAVRSGQVLGSVSPEGELIVECYAGADKAGLLEKGHTGRFQVDAYNYKYFG
                     WLEGKIVSIDNDFTVLDNRPAYRIRCSIDQPRLLMKNGFVGNLKKGMTVQAQFTLAER
                     KLWQLLWDKLDDWLNPQSGSQSPAKPR"
     gene            7648..9798
                     /locus_tag="PKM27_04090"
     CDS             7648..9798
                     /locus_tag="PKM27_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNR06471.1"
                     /translation="MSIPARVKIRQRDITDCGAACLASVSAFYHLKIPIARIRQKAGT
                     DQKGTNILGMIEAAEYLGFDARGVKGPFEALETVPLPVIVHLVVREILYHYCVLYKID
                     AKSVTLMDPATGKFEKKKIQEFREQWTGVAVLISPSDRFRKGNKKTPNSIRFWQLIAP
                     ESGLISLAFIGALLYTLLGLSTSIYVQKIVDFVLVDGNTRLLNLLSVAMVCLLLFQMS
                     FGFLKSWFSMKAGQHIDRRLILGYYKHLFRLPKRFFDTMRVGEIISRVNDAVKIRSFI
                     NEAALGLMVNLLILLFSLAVMFVYYWKLAILLLFILPVYFLLYTLSNWINKKWVRRIM
                     EESAELEAQWVESLNAAATIKRFGVETQWNERTARKFNALLYSVFRSGTYGLALSTGV
                     EWSNRIFTILILWAGSYFVIAREMSAGELFSFYSLIAYFTGPAASMIGANRAVQDALI
                     AADRLFEIIDLDTEQNEDTRICLSPELLDDIQFTRVGFRYGTRKEVFRDLNLVIRKGK
                     STAIVGDSGCGKSTLLSLLQNLYPLQKGKITIGIFELNQMSNASLRKIVGVVPQEISL
                     FDGTIAENIALGEFYPDLRKITEIGHRLGITEFVEQLPGGYHSRISEQGTNLSSGQRQ
                     RIAIARALYRDPEILILDEATSALDPIAESRVRETLNWFKDRDKTIILIAHRLHTIRH
                     CDEIVVLHEGRVSEAGTHENLMNLKGRYFQMFSA"
     gene            9912..12065
                     /locus_tag="PKM27_04095"
     CDS             9912..12065
                     /locus_tag="PKM27_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06472.1"
                     /translation="MKKTGNGKSLDLVAIILLLLLISCAEKKQKRYSDPLTPAEAMQH
                     FKIAEGFHLELFAAEPQVMSPVDMAMDEQGILYVIEMGDYPYMPEPGSAKGLIKALQD
                     KNKDGRVDTAIVFATKLPSATSLLPWKGGLLVAAAPDIFFMKDTTGDFVADIKEVLFT
                     GFFDDNSEAHITSFRFCVDNWVYANNNGQTGVITAPGHPSLPAVTISGGDFRFRLDTV
                     LFEKESGTGQFGMTMDDWGNRFYTQNTWHIQQPSIKGRYLFRHKFLPDQKADVNISDH
                     DLEMFQLTPPPYWRLERSNRRQRNFDSLKLDRREWAEDHFTGASGGTFYGGDAYPESY
                     YGSIFTGEVAGNLVHRDIIAPRSDKPVFSARRGPLEKDREFIASDDPWFRPVNLWLGP
                     DGNLYLIDMYLQHIETPLSIPPDLLADMDFSAGDKYGRIYRIVSDKAPARKMPEFNLR
                     KNSSAELVQNLAHPNQWWRLQSQKLLLERQDQSVVPLLKNMFATHTDPRARLHALYML
                     DGLKQLNADLVSQALKDQSWGIRKQACLLAERYPSLFDPLNSLLSDTSIQVAFQAALS
                     IGEFPPSRSMPALIRVLEKYGSEPLFRSAVLSSLPGSSMPFLNLLFTKSQWLRTQQED
                     SVAFMTDFAYIIGVRRQADEVRALITLLSDTDLMNEGLQKSALAGLAMGIKSLKNKNK
                     ADPGLFSAVQNLKLGKTEKLKSAVDQFMAAIRDTL"
     gene            12092..12490
                     /locus_tag="PKM27_04100"
     CDS             12092..12490
                     /locus_tag="PKM27_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HNR06473.1"
                     /translation="MDNLISRRLFVRKWFFSGFLLGGAAALAGGCASNEKKSPGPAEK
                     EAAAEEKSGPPSGPETNHTGDPCTDYSGVSEADLKIREAMGYVPLSTVEGKQCSNCNL
                     YLPGTACGSCQLFKGPVTANGNCTYWAPQV"
     gene            12513..13898
                     /locus_tag="PKM27_04105"
     CDS             12513..13898
                     /locus_tag="PKM27_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06474.1"
                     /translation="MKSYFSYGLFRIKIRDRICLLLGSVLFFSLKAQELRIGTFYTDA
                     TPPIGSPVAYAIARSVTEPLSAKGIVILSDQKPIVLCAVDWIGISNEGLEVFANQLAL
                     AANTSPERVSIHALHQHDGVRCDFTIERILAEYGLGGRHFDNVFLNKVIQNTSAALKK
                     AMQDNQPFTHIGFGQAKVDSVASNRRILGPDGKVAIIRFSKSVDSAAIHAPEGLVDPW
                     LKTVSFWNGEKTLAVLTYYTTHPQSYYGEGDVTCEFVGIAREARQAKMNGIPHIHFNG
                     ASGNIAAGKYNDGSKARRPVLAARVEKGMTEAWKNTVKMQVSAGMLEWKNLKVKLPLG
                     KNIVESDLRARITNDQNTPPQKFVAAEKLAWYQRSKAGVKVNISVIKLGTIHLLNLPG
                     ELFVEYQLAAQEMKPGEQVCTAAYEEYGPGYIGTKISYTQGGYETSGIVSGVAPGVEK
                     VLLRAIHKVLK"
     gene            13913..15286
                     /locus_tag="PKM27_04110"
     CDS             13913..15286
                     /locus_tag="PKM27_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNR06475.1"
                     /translation="MDRRTFVKNTAAMAALNPFMERKEVFITPKAPLVKVNHTQSNFE
                     REKLLRPFGFKGGYLTELWQTAVKLQSEKYSGIGLATQSVLYGDADLFVRYSETGGNA
                     LMYALTEKALQQVKETPFSDPVTLMDKIHPGLYEEAKRMTGKRDLNENFLMNALVSVD
                     NAAWLLYAAENGLENFYDMLPEPYKPALSYKNNKVAFMFQVSYGMPVEELVKAVEEGY
                     FIIKIKTGQPGTQSEMLQKDMARLSQIHQALKDFRTNHTPDGKLIYTLDANARYEKKE
                     TLLRYLDHARVIGAFPQILFIEEPLNENNSEDVSDTGVRIGADESVHDERTALQRLQQ
                     GYRLMVLKGIAKTLSKTLKIARLAHERNVPCILADLTVNPILIDWNKNLAARVKPFPG
                     MDMGMMETNGDANYVHWKTMQSRHPAAGAPWTRVNKGVFELDEEYFNRSGGIFEKSTH
                     YEELLKG"
     gene            15294..16421
                     /locus_tag="PKM27_04115"
     CDS             15294..16421
                     /locus_tag="PKM27_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNR06476.1"
                     /translation="MNRRSGFLFLLEKCFRVLVSGIFLFLVQCKPSVPQKGLLTYTEE
                     NGNYKPVTNPAEWEIKRKQVLKGMEEAMGALPSREGLPPMDVEYTDSLEEAAYTRYDI
                     RFVAAVNEILPAYLYIPRDKGENQKFPAMLALHETDSIGKKSVDGQGKNKNLAYAKEL
                     AVRGYVVIAPDYPGFGDLQPYDFSSDRYWSGTMKGIFDHIRCIDLLVSRPEVDSGRIG
                     VIGHSLGGHNALFVGAFDTRLKAIISSCGWTPFADYDIGPFAVEKYGGRLGPYAQERY
                     MPLLRDKYHLEDALFPFDFTDIIASLAPRAFFSNSPERDDNFDVRGVRKGIGEAMKVY
                     RMFVQEDRLQVRYPDTKHDFPEEVRREAYRFLDSILGHKGE"
     gene            16436..17056
                     /locus_tag="PKM27_04120"
     CDS             16436..17056
                     /locus_tag="PKM27_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNR06477.1"
                     /translation="MAPSKTKIIHHLSKDPVLQPVLETVPFPKLQPSSVPLSEALIES
                     IVYQQLSIKAAATIYQRFLDRFENKQLELKKLARMKTESLRSVGLSYQKADYVRNIAS
                     FFLQKENQDIDWPTLPEEELLKRLVTIKGVGQWTVQMILINPLGKLDVFPALDLGVQQ
                     GIVRLYELKETGKNLISRMHEIAEPWRPYRSVACMYLWRWKDQFRK"
     gene            complement(17086..17652)
                     /locus_tag="PKM27_04125"
     CDS             complement(17086..17652)
                     /locus_tag="PKM27_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR06478.1"
                     /translation="MSYTESTMLPLGTRAPEFYLPDVISGKSFSLDELKSGRATLIMF
                     ICNHCPYVKHVNPEIVRIAKEYIPRGVSFIAISSNDAQKYPADGPDQMKITAAEQGYP
                     FPYLYDESQEVARTYDAACTPDFYLFDDELKLVYRGRLDGSRPKNDVPLTGTDLRKAL
                     DAVLNHEPVTDKQYPSGGCNIKWKPREA"
     gene            17735..18184
                     /locus_tag="PKM27_04130"
     CDS             17735..18184
                     /locus_tag="PKM27_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR06479.1"
                     /translation="MITLMRTTPGHPEFRRLVHQLDETLKELDGEDHVIYAPLNQIHV
                     LKHAVLAMEGDEALACGAFKHYSRELAEIKRMFVVSSKRGEGLGALVLNELESWAKEL
                     GHSACILETGRNNPIAIRLYLKSGYEEIPNYGPYEKLMSSICMRKNL"
     gene            complement(18194..18514)
                     /locus_tag="PKM27_04135"
     CDS             complement(18194..18514)
                     /locus_tag="PKM27_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR06480.1"
                     /translation="MHISLETAQSRLSGTGGLFESLFEHGSLRVEIYKPVKEDHQKPH
                     DRDEVYIIISGQGRFINGDQEVLFGPHDFLFVPAGQEHRFMDFTDDFSTWVIFYGPVG
                     GEKG"
     gene            18643..20016
                     /locus_tag="PKM27_04140"
     CDS             18643..20016
                     /locus_tag="PKM27_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06481.1"
                     /translation="MTKKFRSGRRQFIKQGLQTSFALSLPTIVPARVFGRFAPSNRIT
                     VGAIGNGRISRTHDMPGVWRQDYAQIIAVCDLDTHRAQDGKKLVNEYYSKKYGKPYDG
                     VTAHYHYEELLQNKDIDAVLISTPDHWHARIAMDAAKAGKHIYMQKPASLTISEGRKV
                     SDAVQKSGVVFQIGSQQRSSTQFRYAAELVRNGRIGQLKTVSVGLPGDPSGDMEPEMP
                     VPQNLNYEMWLGSTPEVYYTEKRVHPQNGYDRPGWLRCEQFGAGMITGWGSHHIDSAH
                     WGMGTERTGPIEIWGHAEFPKSGLWDVHGIFRTEARYANGVHMIVSNELPNGVKFEGT
                     EGWIFVTRGNYRATASDPVVNADGTKPLDASDPKILTSEIGPNEFHFIVSSNHHANWL
                     EAIRDKKQPIAPVEEAHRSCSACLLHHIAMKLDRKVHWDPVKEDFVNDPQASALLSRP
                     QRKPYGI"
     gene            20038..20466
                     /locus_tag="PKM27_04145"
     CDS             20038..20466
                     /locus_tag="PKM27_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06482.1"
                     /translation="MNSLGILSFSETAAGFIDHSLDKSRWTHEAHITTAIWFLLNYEK
                     QEALCRLRSGIISYNLATGGENTGSSGYHETITVFWWTLLERFVQANPGKDYDQLCRA
                     FLNSRYAHRETPFEFYSREKLLSARARAYYLAPDIKEIHL"
     gene            20466..21839
                     /locus_tag="PKM27_04150"
     CDS             20466..21839
                     /locus_tag="PKM27_04150"
                     /inference="COORDINATES: protein motif:HMM:NF027805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNR06483.1"
                     /translation="MKNNLFLLCCSFIFMHCSAPMPVNPNPYQIITLDPGHFHAALVQ
                     KSMYPGVDSTVRVYAPGGPDLDMHLQRIKNYNERKDQPTRWNEVVYTGPDFFERMIAD
                     KKGNLVVLSGNNRLKPDYILGSLEAGMHVLADKPMIIQYEDFEKLKTAFELAKQKNIL
                     LYDIMTERDEVSTLMQRTLSMNKDLFGELEKGSLEQPAITKESVHHLYKTVSGNVLVR
                     PSWFLDVRQQGEGLVDVMTHLVDLVQWECFPDQIIDTQDIRILKARRWTTDLNLEKFT
                     AITQSPAFPDFLHQDLVQDTLLKVYCNGEINYTIKGIHAKTSVIWNYKAPEGSGDTHY
                     SIMRGTKSDLIIRQGANENYQPTLYLEPRRKGDTSIALALKEQIKNLDFDGLGIQFIP
                     TEHGWRVSFPANMLEGHEAHFARVMEKFLSYLKDKNMPAWEVPNMLAKYYTTTKALEW
                     AMKDNGL"
     gene            complement(21888..23453)
                     /locus_tag="PKM27_04155"
     CDS             complement(21888..23453)
                     /locus_tag="PKM27_04155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR06484.1"
                     /translation="MMDLGRRQFLQYGSLLSAGSLFPPAFSFLSMNPVPDLPRATPES
                     TGVLSESIIQYLTEADKSGVEHHGFILMRHGKVIAEAYWKPFERNQVHTLYSLSKSFT
                     STAIGFAVQEGKIRLTDKVISFFPEHLPETVSENLSRMEVRHALNMSTGHIKDTIPVM
                     RAAQGVSWIKSFLACPVEKEPGTHFLYNTGATYICSAIISRVTGQSLQEYLGPRLYRP
                     LGITQSDWEVSPEGINTGGYGLRVTADDIARFGQFYLQKGKWEGKALLGEAWVDEATS
                     SHIASNPGDGDWSQGYGYQFWRCKPGFYRGDGAFGQYCVVMPQYDAVIALNSESSDMQ
                     KQMTLMWDHLLPGMKAEALAENKSKYEELSALSNSLSLTSQLGSNGSPLVNRINGKEI
                     QLAENPWSWKSIKVNAGNKGGHILLDGSRGKVEIPFAWNQWQVNDHRIQNPFNNPNQA
                     PIGSRVAATAGCIPNGSLKIRLKYTEGIHGDLLTLGSDQEHEIHVNFLYSMSEKNPNN
                     KDNRAGLKGRLTS"
     gene            23606..24952
                     /locus_tag="PKM27_04160"
     CDS             23606..24952
                     /locus_tag="PKM27_04160"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNR06485.1"
                     /translation="MKRIVLLLFVLETTFNLFGQSTPVVSGPMLGPVELRDALIWLEV
                     SPSVRTVSLQYRKTGTGVYKTKAYRGPLGNEFNPIQIQVGGLDLNTEYEYRFNLDGKP
                     SAASGKFRTKDLWQWRKPAPDFSFLTGSCAYVNEPAFDRPGRPYGGDSTIFETMAREQ
                     AVFMLWTGDTWYTREVDYYSKWGLWYRAHHDRAVPVLQPFLKAMPHWASWDDHDYGPN
                     DIGSNYILKEESRKVFMHYFCNPSYGENNQGIYTMNSWGDVDIFLLDDRWWRSADQLP
                     DSIHGRPNPDKIVIGSQQMNWLKNSLVYSQATFKIIVLGSQVLNPVSAFDKFLDFPVE
                     YQELMDFLMQHKIDGVLFLSGDRHHSEIIRVERPGAYPLYDITVSPLTSGTHRFAPNE
                     QNNPYRVLGVDQKQNYGRFTISGPRHERVMKVELLGVKGDLLADWQVHEKELKFQK"
     gene            25040..26248
                     /locus_tag="PKM27_04165"
     CDS             25040..26248
                     /locus_tag="PKM27_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR06486.1"
                     /translation="MKKKWLLYAFFSALIHVFQACDNNQFPPETIEVRGLFSLTGNWS
                     SLGIASQAAIDLAAEDINDYLEDRKSGFKLSVSVADTKLDTGLAKTLFTSAQEDKMGF
                     IIGPQSSAELSAIKPLSDAAEIIVISQSSTAGSLAIAGDAIFRFCPSDKIEGAAMAAT
                     IAKRGFKGLVTVARDDAGNKGLQTSTGAAFTEKGGQVAAIPPYAATLTDYRTVVASIK
                     EKLGTLLAVHGMDKVGVYLASFDEGTEIFRLAAMDPVLSNIKWFGGDGAVLSTVFLND
                     AEVADFAIKTSFFAPSFGLPVANEADWKPVAARIKARTGTEPDAFAIAAYDALWTIAY
                     TIEANQGTTKGFSNLKNHFVEQANSRSGIIGARALDEAGDRANGTYDYFGIAKEGNTY
                     VWKLVGKSTD"
     gene            26586..27413
                     /locus_tag="PKM27_04170"
     CDS             26586..27413
                     /locus_tag="PKM27_04170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR06487.1"
                     /translation="MIRKLFYQLSPLLRFLVRYVAYLPADLILGRKNKILPPRRLIYT
                     GRGDFERQGREWLEFFIREAGLQPSSSVLDIGSGIGRIAIPMMDWLKGPYTGFDAVKA
                     GVNWCRKNISSRRPNFFFQFVDLRNDLYRHKGRNAAHFDFPFEPKSFDFICAISVFTH
                     LLPEETENYFRQIRKVIKPGGYAVLTFFLTDEETHRLMAKNKDGLLFLYRFGHYSLMN
                     LSVPSANVAYQREYIYALAESNDLKIIKEIKGSWCGREKSHYLDFQDILVLHKPAGA"
     gene            complement(27445..28953)
                     /locus_tag="PKM27_04175"
     CDS             complement(27445..28953)
                     /locus_tag="PKM27_04175"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNR06488.1"
                     /translation="MSAEPILVLCRMTVLLILFHGFYLLFLRKLTFYKEIRFYFLTGL
                     VLSLVLPFVSFEMSVSGSGPVLQELSNTIPALSTPVNPYGIRELTPAGNPSFQWLFWA
                     WFAGMLFLAARLIIRILSFRRIKQNTRPVHMAGVRIHQLQAPHAPFSFGKDIFIDSDA
                     FRGKDYEKILLHEFIHVKQRHSLDMMLAEWITIINWYNPAAWSLKRAIRENLEYLTDD
                     AMLRLGVDAKAYQYLLLQATGTPRFSFASAFNFQSLKNRIIMMNSRKSHRLQRIRFLL
                     FLPAMALALFAFRTIQEEAPVIIRPFADQPAEVPKDTVPGKSSVQRSSFSYNGFGDNS
                     RIRSIRVENKNAEVHLKNGTNENYNLNDEEQALEFQRKYGELIIPPAPPAPPVAPAPP
                     VPPVPPIPPVPPIPPMPPINENGYAPPVPPVPPVPTVPPVPPAPPVPPAPPVPPVPPV
                     IEWDLPDEVTHFNINHDKVKIKYKNGDIETYDLNDPKEKEKFKEKYKLKNKD"
     gene            complement(28950..29315)
                     /locus_tag="PKM27_04180"
     CDS             complement(28950..29315)
                     /locus_tag="PKM27_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNR06489.1"
                     /translation="MHKLSPPEEQAMKVIWQLGETHIRAILDALAGDQVPYTTLASTI
                     KNLEKKNFVSSRKIGNVSLYKPLISEAEYKKANLNQMVRDYFDNSYKDVVNFFVEQQA
                     LSPKELKEIISLIEKGKKS"
     gene            29527..30882
                     /locus_tag="PKM27_04185"
     CDS             29527..30882
                     /locus_tag="PKM27_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNR06490.1"
                     /translation="MTVFIVLFTLVMLSHGLHLLNPGILHPRFLTTLRWLTIGALVWI
                     GLKKNNLTTWIFLSMVIGAEIGHDYPGVGANLKVISDIFLRMIKTIIAPLLFSTLVVG
                     IAGHADMRQVGRMGWKSLVYFEVVTTIALVIGLLAINISKAGVGIDLSAADAQTVQVA
                     KQSWQDIVLHTFPENIAKAVAEGQVLQIVVFSILFGLGLSMVKSGRHKENMLQWCESL
                     SEVMFKFTHLVMYLAPLAVGAAIAYTIGKMGFDVLRNLFLLLLTLYAALVVFVLGVLL
                     PIALYIKLPIKKFLNAVSEPVLLAFATASSESALPHAMKALEGFGIPRKVVSFVLPTG
                     FSFNLDGSTLYLSLALVFVAQAAKVDLSIGEQIVMMLTLMLSSKGVAGVARASLVILL
                     GTAVSFNLPTEPIFIILGIDALMDMARTATNLLGNCLATAVIAKWEGEVDFENLPEPV
                     A"
     gene            complement(30929..31846)
                     /locus_tag="PKM27_04190"
     CDS             complement(30929..31846)
                     /locus_tag="PKM27_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06491.1"
                     /translation="MNTPDFIKRDDFPMPDRYKVFLSEQDYNQILRIALDCLQKLEYP
                     VQRIEEGDIVVLFREQEAHCYLDNLVKKIAPLSAEDRAKEIERHFRQLRIQPEAYDYF
                     YKDFDHAKQYLKVLVRPVDTTPNPGEYVHRVDFPGTHTYLVFDFNHQFNYIRRDRIAE
                     WEIAEEDLFHIALSHISREEVQTREYLLGDRFPLYLLFNANFSAAFAIELEKNLRLSA
                     GNLGCLVAIPAKNIALVHCIEGPQVLQLMALLFPEILRIFQDAPGGISTDFYWYRKGR
                     FEPFPQKPIRENELTIHLPQKLALLLNQN"
     gene            complement(31936..33042)
                     /locus_tag="PKM27_04195"
     CDS             complement(31936..33042)
                     /locus_tag="PKM27_04195"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNR06492.1"
                     /translation="MDKYHLPGQRLLSLDFLRGLIMVLLALESTRLYEHLQEATVPGG
                     FLHQALDQFFHHTWNGLRFWDLIQPGFMYMAGVAMAYSLHKQQENGMSWNESFLKIFK
                     RCGWLFFWGVLDYAVRPHGLSFELWDVLTQLSFTTLVAFLIFRWPAAYQILACAGILL
                     LTEILYRYTQVPGFDQPFTDQRNFGNYMDLVLMNKINKGGWVAINCIPTSVHTIGGAL
                     TGIWLIKYKKDALSGLLLWALIALAAGYFLDLAGVTPIIKRIATSSFTLASLGWCLLA
                     MAAVYWWVDIRDHKKYVWFFTVVGMNSIFIYLFFEIVGSRWFNGYVTAISGGLLALIS
                     TPQWLSAILSSLCIFALEWGLCYFLYRKGIFFKV"
     gene            33184..33540
                     /locus_tag="PKM27_04200"
     CDS             33184..33540
                     /locus_tag="PKM27_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06493.1"
                     /translation="MKNFGLLLILTLALACERAAGPLNGVCFTLTERQCAMDPFNSYV
                     KDTKTLEERANGVAAYLRDQGIALSEVKSNPKFTGAVCLACTCPSEYSYRLVISSEDT
                     VKLKTLDLALTEKDCD"
     gene            complement(33579..35804)
                     /locus_tag="PKM27_04205"
     CDS             complement(33579..35804)
                     /locus_tag="PKM27_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR06494.1"
                     /translation="MKRGGKLAWFLLFLHAWTLPAQQGIEKLGPSINSDAYDEISPVI
                     SRDGQILYFTRVGSADFNRTLIQEEIDLSKTETDSTYLVVLKEIYSQIAGRRIESPVS
                     ASINQDIWIAESRDQLFDQVYHPGFPINNALPNSVCSLSPDENRMIVINQFGRDGSLY
                     KGFSVIERQVDGAFAFPEPIHIRDHYSNSPEVNLCMTRDGDVILLSVNRNDGRGDNDL
                     YVCFRVKNNLWSEPLNLGSVINTPFREITPFITDDKTTLFFASNRPGGPGGMDIYMSR
                     RLDFSWTQWSAPELLNPPINSIFDDSNPVLMDPYTRLYLTSRRDGTSDIFVADFAGKS
                     PDFRPEIPFLSPPMVFRGRMINSLTGEAVASNLTYGPSRMKKHENTFKSPDGLFEISI
                     DKKEKYRLTPYKEGFASKGLLIDIPLLDQFDNRIYDIDFLMFPLEKEQKIELKNIYFE
                     KGTPVVSPSSFESLDGLAALLNQNPGMQIRIEGHTDSVGKFYELLDLSRNRSNAIKKY
                     LVGKGIMSGRVSTAGYGGTRPIAPNDTEENRAQNRRVEVIVTRSGAPSKVQYKDLPDP
                     VIRKASVPLAQANPPIEPLPMTVSRSLPEMRTKTKQNPVADPPIRNRRDSPDEVSRPS
                     KKPSQNSGLSITSHDRKSQAVYTIDFIPGQLALLQNAFAEMQKLIEFLNRNPEKKIRL
                     SGVCNSQEFSTKPAGFCLQRALGIKEYLVYKSVDPSRVAISEEFLMGEGTPGVMVFLQ
                     D"
     gene            35920..36540
                     /locus_tag="PKM27_04210"
     CDS             35920..36540
                     /locus_tag="PKM27_04210"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNR06495.1"
                     /translation="MKVRKGLLIISFLAYAWYAVGQNGADPVTEEKRVINGQVVKLMI
                     SGTDTLYIADLQDVHISSPRQFKNYEEYRMYMRYKRYAADVYPYAKEAIEVYRQIKEE
                     TEDLKRGKRKKYIRSVNKEYKNDYKETFKNMTRLQGKVMIKMIERELDVSFFDLIKEV
                     RGGFTATYWDTFSRLYGYRLRDKYEKGEEPILDAVLQDFNITAKLK"
     gene            complement(36627..38849)
                     /locus_tag="PKM27_04215"
     CDS             complement(36627..38849)
                     /locus_tag="PKM27_04215"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNR06496.1"
                     /translation="MFIRLQAFFITISLVAGASPAGSQNSLNTPYSSSRPTTKAYLLS
                     KAPVLDGEILEDSVWQTIPPFVELIQTQPYLGKPVSEHTEIRIAYDRDFFYLSAACYD
                     ATPDKIIISDSRRDASLDATDAFLFILDTYKDGQNGFVFGTNPAGIEYDAQVDNEGQS
                     NPNANRQQGGRVGGINVNWDASWTVKTKVDKNGWMAEFAIPFRTLRYNSGKNQVWGVN
                     FQRNIQKNNEAAYWSPIPVEFNLNRLSLEGTLTGLDLENPGNLKVIPYVLGKTTSNNL
                     VVPLKTTTDANFGGDIKYSITPALTLDLTYNTDFAQVEVDDQQVNIDRFNLFFPEKRP
                     FFLENAGQFGVGSTGEVDLFFSRRIGIGSGGVQVPIIGGARLSGKINHTNLGLLSLFT
                     DEVDGAGIAKNNFSIARVNHEFAKRSTIGGLFINKQGMGPGEDHYNRTFAIDGKLGMG
                     NKAQFSGFFAKTATPGIKESNHSFKLLYNYEWNKLNVIMGYSELGTGFNPEVGFLLRN
                     SFRKPEATVLYHWRMNGKAGLLELRPHISYRSYWNYGNKKLETGFLHIDNHWEWESGL
                     EIHTGINFTTEGVFVPFEISSGITVPSGTYNHHEAQLVFMSNTSKNVSVNIRSIIGGF
                     FGGRRTSTTGSVNVRLGDKFTSEWGINNNVVRLPYGDFNTNIFRSRISYSFTPRLYVQ
                     SLIQFNDVLDKWSMNMRLGWLQQSNTGLFLVFNENRGLDRFDNRSFTLKYSRMFDVIK
                     "
     gene            complement(38927..39949)
                     /locus_tag="PKM27_04220"
     CDS             complement(38927..39949)
                     /locus_tag="PKM27_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNR06497.1"
                     /translation="MSKPFQRIFNAGSILAGLFLLASWWSPRIDPRQFWPAAFLGLVF
                     PYLYLTCLILAGLGLLWKQHKAWLLVLLLLLSAGLFRKSFFNFSFPPGEEGIRLLTHN
                     IGSSFKSNRAPEWAFYHRLNPDILCFQEWHRKSPAFRFRDSLIKKYKHTQLTGTNFWP
                     IFSRWPIIREGELKSKALGNGCTWADIVYQKDTFRLYNVHLVSNKISDQTEQLLQTRD
                     LTDRSTWQKVKNVLRRYKRSLLLRADQAAELKKHMRQSPHPLVLAGDFNDIPASYVYR
                     LLHEDLMDGFLQGGNGLAYTYAGKLPFLHIDNILISKPLQVNRVEIQRVRYSDHFPVL
                     AEIQIH"
     gene            complement(39970..40863)
                     /locus_tag="PKM27_04225"
     CDS             complement(39970..40863)
                     /locus_tag="PKM27_04225"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNR06498.1"
                     /translation="MFDSIWQDFKYHLGKGTRLNQLMLVNVSVFILVLFVRIGFNIAG
                     EEGRAGFASFTEALSFHSNTGYLLTHPWILITHMFLHIGIWHLIWNMLMLYWFGNIVG
                     DLIGNRVILPLYLVSGCMGVFSIYIFSQIFAYPSAEVVAYGASAAVMGFVLAATIIAP
                     DYLMHLILIGPVKLKYIALVVVLLDLVGLAENSNTGGHIGHLGGGLGGILFIWWLKNG
                     SALPGWPFTNKPKTKIIPIRKAVSAPAAGRKSKLVKPMPEEDDEDQLRNEIDRILDKI
                     RTQGMDSLTKEERHTLEKASK"
     gene            complement(40872..41492)
                     /locus_tag="PKM27_04230"
     CDS             complement(40872..41492)
                     /locus_tag="PKM27_04230"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNR06499.1"
                     /translation="MIRLTDVVKQLLIINGILFLISRLPVYSQLPDLALYYPGSSDFR
                     PYQLVTHMFMHGNLPHLFFNMFMLATFGPMLEALWGSRRFLFFYLFCGIGAILIHLLF
                     WYFEVSQLDVLQHARYMQNSGSVVGASGALFGVLVAFGMYFPNQQLMLLFPPIPMKAV
                     YMVIFAIVLELVMVAQGAQTQIAHFAHLGGALFGFLIILYWRKTGK"
     gene            complement(41726..43162)
                     /locus_tag="PKM27_04235"
     CDS             complement(41726..43162)
                     /locus_tag="PKM27_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNR06500.1"
                     /translation="MKSHFLLLLVWFFHLSKAQVQEYYPDPDPLIQQKLEQWQDLKFG
                     LLMHWGPYSQWGVVESWSICPEDVGWAAWAWKKGITEDYDEYRKKYEELPLTFNPTRF
                     NPEKWAAAAKAAGMKYLVFTTKHHDGFNMFDTRYSDYKITGKLVPFSSNPRSDVTKEI
                     FDAFRKEGFWAGAYFSKPDWHNNDYWWSKFPPADRNPNYSINKYPERWKNFADFTRNQ
                     LNELASNYGKLDILWLDGGWVRPKTDEEIKAQLLEVYEGSRYARNPQSQDIDMAGIVR
                     EVRQKQAGLIVVDRAVPGVHQNYLTPEQHIPEKGLPYPWETCMTMANSWSYVPDDLYK
                     PADELIEKLVDIVSKGGNLLLNIGPGPDGEWDPAAYDRLKKIGTWMKINGEGIYNTRM
                     GPSYIDGDRVRLTQNKDGKASYIFLFDFPKDKIILTKLKPGPISGVSMLGVHSDLKWE
                     QTPAGLEIVLPSELQSRGEYVWTLKVEY"
     gene            43396..44202
                     /locus_tag="PKM27_04240"
     CDS             43396..44202
                     /locus_tag="PKM27_04240"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNR06501.1"
                     /translation="MAAKLALCIGINDYPETGSDLAGCVNDANDWADTLTARGYVVKK
                     LLDKKATRANILGEMKSVISAAKKGDSILIQYSGHGTYIPDQDGDEPDGTEECLCPYD
                     LKTRGVITDDELFDLFNSKAAGVRLVMISDSCHSGTVTRFAPPAVRGRKAPQTRVKFL
                     PPASFLSSRELNKFGVSRAIRKSSPPGRYGGLLMSGCQDVEYSYDAYFGGRPNGAFTF
                     AALATLKKLKSGASYKDWHKAIRSMLPSQQYPQSPNLFGSSTMKKWKVLQ"
     gene            44319..45131
                     /locus_tag="PKM27_04245"
     CDS             44319..45131
                     /locus_tag="PKM27_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNR06502.1"
                     /translation="MQGYFYTPPATRFYYAEAQQKKRIVLHFTAGNLAGDMNQLTIQD
                     YHVSVPFVIARDGKIYQLFYSKYWSHHLGLKSGNPAKVYDRQSIGIEISNYGWLESRD
                     GQLETAYSRQGSNPSDVYCGLGDTEAYLQLNRRYRDHEYYASFTPAQLESTIVLVRYL
                     LALYSIPPSFLPPAERYEFIPSNMGFAGILSHVNYRDNGKWDIGPAFDWEGLIQGVQA
                     ESFVPVLNPLRSVRGKPSQQEKEFAPAQNRGSKYKPGSRTVIRQYNPFDWEE"
     gene            complement(45137..46177)
                     /locus_tag="PKM27_04250"
     CDS             complement(45137..46177)
                     /locus_tag="PKM27_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNR06503.1"
                     /translation="MKRSQFLRTSLMGTGALLAGSPLLSSLASCKQTSTLSNRDRMLQ
                     WLEGKTEPGYTPAAFFLHFGDDFKLGSAAAQEHLRYFRHTGMDFVKIQYEQQYPLLDF
                     LKTPADWSKLRPNPVEFYQPQLQAVKEIVQASKKEALVIMTLYSPFMWAGHSATREVL
                     LKHMEEDPEAVKKGLDILTESQLIFVRACIDLGVDGFYMSTQGSETGQFSDPRLFTEY
                     VKPSDLVAMKEVTAACPFNILHVCDYNAPYASYDAVLDYPGQVVNCNPQLTGRLLTWQ
                     EISEMFNRPCMGGMDRHGVIGKGSRTEIENEVRNVLKQVPDQFILGADCTVPGDTDWD
                     RLKAAISVAHGV"
     gene            46512..46811
                     /locus_tag="PKM27_04255"
     CDS             46512..46811
                     /locus_tag="PKM27_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNR06504.1"
                     /translation="MKKLKNIHPGEILLKEFLIPLDISAYRLSKEISIPQTRISEIIK
                     GNRRITADTALRFSKFFGNSAKFWLGLQDDFDIEEEKQNKQKEINSIKPLESKVA"
     gene            47073..47786
                     /locus_tag="PKM27_04260"
     CDS             47073..47786
                     /locus_tag="PKM27_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06505.1"
                     /translation="MKQTFIFILTIFILSSCNEEKKSDSNITIDTVSNTTSNNTVPTN
                     QLTPNLTFDCSIFKFKSKKEQADTLTKYFGKKQLATDEKFFCAFPNSFNEMQNIFGFD
                     DIRGEAPMYDYPMGENIIRYFANLNTIDKEIYYNKYINICVDGIWEADNIREAFGFSN
                     RLANDTEAGCLSLSKRTDKEIKSVFHFIFDGPHPKNEYNEKIYNELLPIVTKQNDRLG
                     KLLTESYKKVITEDGGNGH"
     gene            48001..48537
                     /locus_tag="PKM27_04265"
     CDS             48001..48537
                     /locus_tag="PKM27_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06506.1"
                     /translation="MIKQLTIFAFILTICLISCGKTNTESKKTEKLTTIQIDTSVITI
                     LPLDTNLHWIFKTGMPTELTTEDLLKIETILKKCVNEYNPEQERQFKEINDKHPEYKL
                     ERKSFIIDLARYKRQYMAIVNSKGEKEIWVNCFCGNWDKNSRTSPVMVDDGGNCYFNL
                     KINLTTGQYYELMVNGDA"
     gene            48756..49244
                     /locus_tag="PKM27_04270"
     CDS             48756..49244
                     /locus_tag="PKM27_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06507.1"
                     /translation="MDASKNPRLFLLEGLQRFIKQVTKLDGVIRISLLGSIVTPKTNL
                     KDIDVLVLVADHIDLAPLATYTRQLMGQVQSINRGADVFLADERGNYIGRICHWRDCR
                     PGIRRACQAMHCGRRTYLYDDLSVVTLNAEMVRNPPVTLWPTVEIRCPLPRDVREWIE
                     KL"
     gene            49668..50027
                     /locus_tag="PKM27_04275"
     CDS             49668..50027
                     /locus_tag="PKM27_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06508.1"
                     /translation="MNKSSTSLTVFGVYLLGMGFGLVFMPNTLIGILGLPPTDEVWIR
                     VLGVVTLVLAYYYIQAARTNLRTFASWTVPARILVFFFFTGFVLAGWVGPVMSALGAI
                     DLVGALWTAWALRKEKS"
     gene            complement(50110..51153)
                     /locus_tag="PKM27_04280"
     CDS             complement(50110..51153)
                     /locus_tag="PKM27_04280"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNR06509.1"
                     /translation="MIATKAYAAYQADQPLRPFEFNRREVGPHDVHLEIRYCGVCHSD
                     LHRARGEWDGTHFPLVPGHEIVGTVKSVGPAVTKFKPGNLAAIGVIVDSCRSCPSCRQ
                     HQENYCDEGPTGTYNVMARDGSGYTRGGYSSQIVTDERFVYHISSRLDQSAVAPLLCA
                     GITTYSPLKYAGVGKDTRVGVAGLGGLGHMGVKFAAAFGAEVTLISHTPSKETDGKRL
                     GAHHFLLSSDQAQMKKHNGQFDVILNCISAQHDYKKYLDLLGVNGKMLVVGLPPEFPV
                     LDPFALIKNRRSIIGSMIGGTEETQEMLDFCAEKNIVSDVEMIPIQKIDKAYDRMVKG
                     DVRYRFVIDMASL"
     gene            complement(51268..53310)
                     /locus_tag="PKM27_04285"
     CDS             complement(51268..53310)
                     /locus_tag="PKM27_04285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNR06510.1"
                     /translation="MKYLYYFFLALLPGMLTGQDASVSFEEWISLKQSGTPQISPDGK
                     HVLFSVTSTDWKENVYDTEIWLSKNGQAPFQLTRTPKGSSFGARWSPDSKWIAFLTDR
                     GNKSQIHLLSLEGGEAVAISNETEGINSFTWSPDGKKILFSKTESETKRDKQIKERFG
                     AFGTEGEEFRMNRLWTIPFDKDSIDRYGLYPCYSDSVSKTKLPCFNFPKATAVTDSNY
                     SVAGYGYSPDGKYIAYTRLPDPLINSGVFGDIALYETGTRSSKILVDNPSGDFFLGWS
                     PDSRQLIYSSAVDDTVSFYYKNNRFFIRHLDSASAKEIGKAFDENKSYLDWTRGGIWF
                     GASQKTASQLFRLDPEIDLWSKSEVPYDVFNTATFSKDGQTCALSGRMYDGLNEIALY
                     NFNTSSRQLTFFTDQIKNWKTPTSEIIRWQSRDGTEIEGVLIRPRDFRTDKKYPLLCL
                     IHGGPTGVDRPEPTPSYVYPVMQWCEKGALVLRVNYRGSAGYGQLFRSLNVRNLGIGD
                     MWDVMSGIDYVNKLGIVDTTKMGCMGWSQGGYISAFLTTHTNVFKAISVGAGISNWST
                     YYVNTDITPFTRQYLQSTPWSDPAIYLRTSPMNNINKASTPTLIQHGEFDRRVPIPNA
                     YELYRGLQDKKVPSKLIVYKGFGHGITKPKERLAAIWHNWQWFNKYIWGEEVVLPE"
     gene            complement(53332..54663)
                     /locus_tag="PKM27_04290"
     CDS             complement(53332..54663)
                     /locus_tag="PKM27_04290"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNR06511.1"
                     /translation="MFLSGIKYFLWGFLAAACFSCSTPAYLGSVKIAENKGERFGPSE
                     PSIAVSPVNPDIVVAGSILDRVHYSTDGGKSFSHSRLLSPLGVYGDPVVWADHEGRFY
                     YVHLGNPDGRGRQSGRWLESLVIHQSSDGGKTWDQGSAIGTNPPKNQDKPWISSNPNN
                     GDLVVTWTEFDQYGSEKETDRSRIRFSLSKDQGKTWTQAVTLSDHEGNCLDDDLTPEG
                     AVPGFGPSNEIYVCWAYDQKLWFDKSADGGNTWLLADQALVDQPGGWSLEVPGVGRAN
                     GMPVLATDLSPGKYRGSIYICWADLKNGQNNMDIWLLSSRDGGNSWSERVRVNQDRTS
                     SIQFFPWLSVDQSTGYLYIVYYDRSRHRNLQTDVVVAYSKNGGKSFKELRLEGNTFTP
                     PGNEAFFGDYNNISAANGRVRPVWTSYMDKKLSIWTALIETKSQKKQKARS"
     gene            54913..56058
                     /locus_tag="PKM27_04295"
     CDS             54913..56058
                     /locus_tag="PKM27_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNR06512.1"
                     /translation="MDHRSPLRIIHCLWLIYCLNLPLSGFSQVDKKATAETKALYGNL
                     MKIRSRGVMFGHQDALAYGLNADLSRWIGQPVKTDIRSDIRTLTGQHPAVVGHDLGHL
                     ELGKPENLDAVPFDQMRESIIAVYRYGGINTISWHPNNPLDLTKTTWDRVDSTIRRIT
                     ADRKNLRNYKKILDKLAVFFKSLKGPGGALIPVIFRPYHEHTGSWFWWGAGHCTPSEY
                     KAFWQVTVKHLTRKRKVHNLLIAYSTDNFINEAHYLERYPGDEYVDLLGFDTYHRNAP
                     QSDSAFTANAKRMVATLKKLGEEKNKLYAITETGLETVKEHDWWTRIVYPIISDTGLS
                     YVLVWRNGRADHYYAPFEGQQSAEDFKSFFKLPQTLFEKDLLNLYKP"
     gene            56097..57269
                     /locus_tag="PKM27_04300"
     CDS             56097..57269
                     /locus_tag="PKM27_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNR06513.1"
                     /translation="MNFKERLNALKAEKEVLLNRSNEIVLPGNGIYFRYKNPVVTAGH
                     VPLFWEYDLNPENNPFLMKRFPINATFNAGAIKWKGKYLLVLRVEGADRKSFFAIAES
                     PDGISRFTFWDHPVTMPETGNPDINVYDMRLTAHEDGWIYGIFCSERKDPFAPKGDES
                     SAVASAGIARSRDLVNWERLPDLVTPSPQQRNVVLHPEFVQGKYLIYTRPQDGFIEAG
                     SGGGIGYGYSDSMNPAIIEKELILDEKRYHTVYEIKNGLGPAPIKTSKGWLHLAHGVR
                     NTAAGLRYVLYLFLTDLADPTKILYKPAGYFLAPEGDERVGDVSNVVFSNGWIADENG
                     NVFIYYASSDTRLHVASSTVEQLIDYAMHTCADGFRSMTSVKTLNNLIDRNITLQG"
     gene            57283..58473
                     /locus_tag="PKM27_04305"
     CDS             57283..58473
                     /locus_tag="PKM27_04305"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNR06514.1"
                     /translation="MDSKLQSFKLEIREEMQRILEWWRKYSPDERNGGFAGEIDQFNR
                     QLPQADKGSVLHARILWTFSSAYRHSRQEVDLDLAFRAYEFIVQKFYDKTWSGVFWSV
                     RADGTILSDRKQSYAIAFAIYGLAEFYKVTGDAGGLQLAVELYHALESRSLDPLEGGY
                     FEAFTRDWQKLEEVRLSQKDRNDPKTMNTHLHLIEAYANLYRVWRDDRLEHSIRHLLK
                     VFEQRIMDKTNHHLRLFFEADWKPTSERISYGHDIEASWLLHDAAKELQDPILDKRWS
                     AIAIQIADASVGGLQADSSFIHEADPRSGYLDQHREWWVSAEGMVGYLNAWKLSGDRV
                     YLERVYGLWNFIKKHLLDLDRGEWHWGVYPDYTRMPEYKMGFWKCPYHNFRACLEILD
                     LLKE"
     gene            58592..>60910
                     /gene="thrA"
                     /locus_tag="PKM27_04310"
     CDS             58592..>60910
                     /gene="thrA"
                     /locus_tag="PKM27_04310"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNR06515.1"
                     /translation="MRILKFGGSSVGSPERILSVIGILRDYTRKKIPYTVVFSAFSGV
                     TDSLIRMALAAAKGDPAYEAMWTVFHERHILAIESLVDEDRRKTRLSQQFNEMAGTLK
                     NLLQGTYLVMEASPRTMDYVLSFGERASCMIITEAMNQRGLPAECMDAREFIKTDKNF
                     GAARVDHNLTNSLIRKKFKPSKKIRVVTGFISSAKGGLTTTLGRGGSDYTASLLGAGL
                     GAERIEIWTDVDGVLTADPRLVDKAFTIPKLSYVEAMEMSHFGAKVIYPPTIKPAMDL
                     GIPIFIKNTFNPGFPGSLITKEKVKHQHPVTGISSISNVSLLTLQGSGLFGVPGVAAR
                     VFEALAEKGINVMLITQGSSESSISFAVRPEDGQEAKTALDHAFARDISASTVEPAHL
                     EKDLSVVALIGENMRYTPGIAGRMFRSLGKNGINVVAIAQGSSELNISAVISRADEAK
                     ALNALHEGLFLSDTKVLHLFIIGTGLIGGTLIRQIQAQAEYLLKSKHLEIKIVGLANS
                     RKMIFDEKGLNTENWKENLMGKGSPMKLARFIDQMKQMNLSNSIFIDNTASEIVASHY
                     DKILDSSISISTPNKVAASSKFARYNKLKEIADNRGVQFQYETNVGAGLPVLSTLKNL
                     MISGDRILRIEGVLSGSLSYIFNKFKTGTHFSDLVREAREKGYTEPDPRDDLSLKDIS
                     RKICILAREAGYRVELETVELEKILSDKALKAKTVADFLKILEQNDSHYNKLIQDAES
                     RGAKIRVIAKVEAIASGLSSCRVSVGLQEVSPD"
BASE COUNT        15964 a        14488 c        15321 g        15137 t
ORIGIN      
        1 attatgcata gaaataaata tcataaaaga tttggaggat cgtagaaatt cgtccagaac
       61 ccaagaagcc aaacagggat ccacaaatca atgcaggtca aagccaaggt ttatccaaaa
      121 taaaaagaga tagttggtga accgtggacg gtttccgttg tgcacagggc ggtctgcaga
      181 aattagggaa tagttaataa ttgctgcaaa aaataaaact tcagcgatcg aggtctgttt
      241 tgttctggta gatatccgaa atcatgggga caatttcggt gaccggaaga taaggatggc
      301 cgaggttatg gttcagccgg gcgattttcg ccatatgcct ccaatgcctg gccatttcct
      361 tcaagccgaa taaataactg tgcccgggat cgtaaatatg ggtgggttcg ctgaaggctc
      421 cgtttaattc gatcacctta aaattcaggc cattttccag ttcttcccag gaattgaact
      481 ttatatcgta tcgcccataa tagaaaccgg gaatggattg actgagccgg tctatgacaa
      541 cattgagttt ttcactggct ttatgactgc tgtcgtaaaa ggtggagccc cgggcatgat
      601 tgccaatagg cgataagagt atttgttcgt ctttccccaa aatagtattc caaacagact
      661 tattttcttt ttcaagccag gatgactggg gatgataacg gtagcttttg ccaaccagct
      721 ggccggccgt gtgaatacca tccccggtaa tagtcactcc ctttttttca acaatgccgc
      781 tgatgaatcc ctgcgactgg tccggataac gcatataaaa cacccctatc tccaggggca
      841 aagggatgta ttcctggatg aggaaatcaa accccgcacc ggcagcatat tgcctgagtt
      901 cattgagatc ccggaccagt tttacttgtt ttccccggta accccggtcc ggttttacaa
      961 tcatcggaaa agccagattt tgttcatcca cccttttttc aatatccgga agcggttctc
     1021 cttttttgat caaaatggat ttgggcagca tgccgggtgg aaacaataaa tgcatttcat
     1081 cttttgactc cgggctcatc ccgccgtatt taaaagaagg gtttaccgca ttaaaaaaac
     1141 ccagggaccg ggccctcagg ctgcaggcca ggtaccaggg cgccagggac agatataggg
     1201 tccacatggg ccagtattcc caatgaagca accggtgcag ttttaattgg aggcggaaga
     1261 tcaaatgggc tgggcccggc ttagccattt ttccgtaggg ttctgttttg ccaggtcaag
     1321 ataggtgtag agggcacctt cggcagaaaa ttcaacctgt gaaatattat gggttttata
     1381 ctggttttcc ctcaggatcc gcaggccgtt cacggggtcc tcgttttgat gatggaggtg
     1441 gaagtttaaa aggtcctgcc tttggatacg atggtggttt tttataaatg ttgaatacca
     1501 gttttgccga agttgactgg ccgcttcatc atataaagtg acggatgaaa agatataggg
     1561 ttggtatgga tccaatccca tgatgtgttt cccttcgccg tcccagacca ggcggcagag
     1621 tccatgacta tccatataaa tgacctggaa gggttcgatt tcagccaggt cataagctct
     1681 aaaacccggg acaaagggtt ccgatttaaa taagtcaatc aataccaaac cccggctttt
     1741 tctgcgtgcc ggccgggggc tatgcttccg gaaggcgccg ttgagcaata cggccacacg
     1801 atgttggttg tcggaaataa accaggtgcc cccggaacgg gaatcccggt tgaagaacaa
     1861 ctgcctgctt ccgtataaaa ccatttccgg ttcagcggat gggggacagg aggttttttc
     1921 atcccggctg gaggtaagga tcctggtttt tccggaatgg aaataagtca ctgtgcacat
     1981 cgggcccggt aatgaagggt ggagggtgcg accccaaact gttcaggcaa ggccaggccg
     2041 gtcatggatt caagggctac ccggatcagg gcctggtggt ggatgcagtg ttcgaggttg
     2101 tagatcagtt cccggtaata attggtggac accgtagtgg gatgtccatg aaaggaatat
     2161 ttgatatgca gatcttggtt gggacggtct atcttatgaa taataacctg gatcaaccgg
     2221 agcgcatggc ttaaatcggt ttcgatctgt ccctcccgct ttctttcatc ataggaaata
     2281 gttcccaggt aataaccttc atccaggcaa tcgaacattt ccaggatatg acgggtatgc
     2341 atgccaatgg atgcattccc gagcagggga cagggtttta tataaataac ctgaccgctc
     2401 tcttccagta cctgagccag gctggtcagc gcttccttga tggattggat cattgccggg
     2461 atttgcacat ttttaaaagg cgaaagatat cttctgagag ggatacggca aaatttttac
     2521 cccaattcaa acccggcccg gtagcagtgt ttttaaaagg ggaataataa aggagtgttc
     2581 ctacagaaaa ccaacaggag aaccgtgttc aaatgtctgt aaacggctaa aaacgaaaag
     2641 aaacgattgt acaccggctc tccggcattc cgggctttac tttgcgataa aaaacatggg
     2701 gacgatgatc actagcttaa ttttgcctaa aatggagaaa ccgaatccgg taaacgcccg
     2761 actggaactg gctcagcaat tcgtgcaata taccaaccgg aatatattcc tcacgggtaa
     2821 agccggtacc ggtaaaacca cttttcttaa aaacctgaaa caccacactc ccaaaaggat
     2881 cgtcgtggtg gctccaacag gggtggccgc catcaatgcc gggggagtga ccattcattc
     2941 cttttttcag cttcctttcg gaccctttat tccgggacaa gaccggcaag gggacagccg
     3001 ggaaatgaaa ttcagcaggg aaaagatcag gaccatcagg gccatggacc tgttggtcat
     3061 cgatgaaatc agcatggtgc gtgcggactt gctggacggc atggataggg tcctgaggcg
     3121 attccggaac ccggacaaac cctttggagg agttcaggtc ctcatgatcg gggacctgca
     3181 ccagctcgct cccgtcatcc gggaagaaga atgggaattg ctcaggcctc attatccgag
     3241 tgtttatttt tttgaaagcc aagcccttaa gctcagcagg ccgcttaccc tggaactgac
     3301 ccatatctac aggcaggaag atgaaaaatt tattggtctg ctgaacagga tcaggcaaaa
     3361 aaagatgaat ccgcaggacc ttgagtggct aaattcccgt taccggcccg aatttgaacc
     3421 aaccggagag gacggatata ttaccctgac cacccacaat gccacggctc aatccatcaa
     3481 tcagaacagg ttgatggttc tcaaggggac aatgcaggtt tttcatgcgc tggtggaagg
     3541 tgagtttccg gaatcgacct atcccaatga gttcaggttg gaacttaaaa agggcgccca
     3601 ggtcatgttt attaaaaatg acaccagccg ggagaaaaat tattacaatg gtaaaatcgg
     3661 taccattacc tccatccgcg aggaagaaat cagggttctc tgccagggag aagaagagga
     3721 gatcagggta ggccgggtaa gctggcagaa tataaaatat gtgctggatg aaaacaaaca
     3781 actggtggaa cagatcctgg ggaccttcac gcagtttccg ctcaaactgg cctgggcaat
     3841 caccatacat aaaagccagg gcttgacttt tgagaaagca gtcattgatg cccaggcctc
     3901 ctttgcccat ggacaggtat atgttgccct cagccgttgt aaaacttttg aaggccttat
     3961 tctcagcacc cccctgagca atcaaagcat caaaaccgac caggccattt cccatttcag
     4021 cgaagaagcc agtcaacaaa accttgacgc atccaccctc tatgattcca ggaaacaaac
     4081 ccaggaggaa tggatcaggg atttgtttga tttccaggcc ctcaaaaaaa acctggcttt
     4141 tctgtcccgg ctgacagagg aaaacaaaaa tcacctggtt gaatctgccc ctgtcgccgt
     4201 acaggaattg aaaaaccggt tgaatgcgga agtgctggtt gtcgtggaaa aatttcttct
     4261 tcagctgccc tattacctgg aacaggccgt gctgcccgaa gaaaaccagg gtttacagga
     4321 tagaatacaa aagggttgtt cttatttgct cccaaaattg gaagggccct ggatggacac
     4381 cctccagcag ttggacctgg attgcgacaa taaggagatg aagaagaaac tccggcaggc
     4441 cctggatcag gttttgcagg aagcccgcgt aaaaatggaa gtgttgaaag cctgccgtgc
     4501 aggatttgaa tctacccatt acctgcaagc taaaaccaat gccggactgg aaccggaaat
     4561 aaaaaaatct tcgccgaaag ggtcaaaaga agagagtaca agcccggaac aacccggttt
     4621 gtatcaggtg ctcaaacaat ggcgcgacgg cctggcggag acaaacaaac ttcccgtata
     4681 tatgatcatg ccccagaaag caatgaaaca gatcagcgcc ttgcttccgg gcaatcttgc
     4741 cgccctggct tccataaaag gcattggcgc gcaaaggctg gcgcaacatg gagaacaaat
     4801 ccttcatttg attgaggaat attgcaatcg gcatcagctc agcccccatt gccttgagga
     4861 aacaaatttg cccccccaaa agaaaattca aaaaggcgaa acccagcaac ggacctatca
     4921 actttttacc cagggcaagt ctatcccgga gattgcacag gaaaggggcc ttgccgtttc
     4981 cacgattgaa aatcacctgg cgaagtatat agccatgggc cttttgcgag tggagcagat
     5041 catggccccg gacaggatcg gtaggcttaa aacgtatatg gaagaccact cccatgcggg
     5101 caataaagaa atcaaagatc attttgggga ggagttcagt tatggggaaa tccatatggt
     5161 ccgggaatcc atgaccgggc ccgggcaaaa taatataccc gtataaaagg aagtcatctc
     5221 aggacaatat gagccagcat cttgtgcatt aaaatttggc agtttaaatt cgaaacagaa
     5281 attaaaagga tcgcagggac cttgtgatga aatttgaatt ttgaaacaca tgggaattgt
     5341 ctccctgtta aagaacaggg aacattcgga aagtagaatt attataaaag gaaagacttt
     5401 gaataagctg gagtactgac tttttataat aacccgttta cctcaagggt gacagaaaca
     5461 ctatgagaac ggagtccagg cagcccggag agatattcgc cggaccggga aaaccggtgg
     5521 atatcaatcc ggcaagcctg cggcttatcg atttcgcatg gtgattttgg ttggggaagg
     5581 agcattgggc acattgcctc cggaatggta aatgcccttt gcctaatgaa ttcgttagag
     5641 cagtgtctta tataaaaccg gggaatttat atcggcggga gtgcctgcct tgtatgcctg
     5701 gcttccaagc aaaaaatgaa ggcatttttt aatcacgcta aaaaattgaa ttatggcaca
     5761 ttttgaagaa ctgcgtttta atgaaaaaat ggaagtggat gggggatggg tcgtcacttt
     5821 ctcccgggcg gtccttaaaa aaacaatcgt gggatccatc gccttatttg tgatagacaa
     5881 ctggtccacc accaaagagg cggtcctgga cggagtgcag gacggcatga aaacctggaa
     5941 ttaaaatcaa ctcgccttga aagataaaat caataaactg ctggaatacc tggaccaatt
     6001 gcatccgttc atctatatct tttttgtgat cggattcctg tatgttttga aatttgtgat
     6061 caaggaactt tattcattat tttttaatca ttaatcaaac acaactatga tagaactgac
     6121 ttttaatgaa acgacccaga ttacaggggg agcccctacc aggaaaacaa gtttcgtata
     6181 tgacttcctt tatgcgattt cgtatggagt gacctggttt cttgggcagg gcgatgagat
     6241 gagcgaagcc attgtgaggc aggatctaaa gcacgtctgg tagaaaggat tcaaagggcg
     6301 cctggtttaa tccgaattag ccgggaccct gtccctgaaa accaggccta acccttcata
     6361 taaggatccg catttcatcc acaagtattt agggcttata gtatctattt attaatggtg
     6421 ttccgggagg gcctgcttcc cagcaggcct tccccctttt ttccttggac ggaccaggcg
     6481 ggttcagctt taattttcac cttttccttt ggttttacag gtttgaaatc tgttttttac
     6541 atcatgctgg gccttttcct gggcctggta ttcctgttta tcggttttgc cccggtaccg
     6601 gtgaacctta aacttcaggg catgatcagg ccttcctcag aaagggccga actggtttgt
     6661 ttaacggcgg gttttataga ttccatttat gtaaaagaag gagaagcggt ggatgccttt
     6721 caacccattg tctccataat aaataaggaa agggcaacga aaatggatca gcttctaacc
     6781 gaaatcgggc aatgcctttc cgacctccgg gacctcgatc tcctatgcaa ggtcaccggt
     6841 gcggtatccc cggaacagat ttcaaatcct gcatttaaac aagaactttt aaaatatggg
     6901 tttgaggtaa aaaagctgga attaagcttg gccaaatcgg aaaatgaatt caaaatcacc
     6961 aaagaacttt ttatgaataa aatcattgcc cctaaagaat attcagacaa agaatttgaa
     7021 tacttgcacc acctggcgga ggcagaaacg gtcaaaagac agcaaatgat actctggcag
     7081 caggcaaggc aaaccaaagg gcgggaactg aatgccctgc aatcccaact aaaactctat
     7141 caggacaacg aaacctggga tatggtccgg gccccggttt caggaattgt cctgggaaca
     7201 cggtcgattt ataagggatc cgcggtccgg tccggtcagg tccttggcag tgtttccccc
     7261 gaaggggagc tcattgtgga atgttatgcg ggtgcggata aagcgggttt gctggaaaaa
     7321 ggccatacag gccggttcca ggttgatgct tataattata aatattttgg ctggctggaa
     7381 ggaaaaatag taagtataga taacgatttt acggtattgg acaaccgtcc ggcttatcga
     7441 atccggtgct ccatagatca acccaggtta ttgatgaaaa acggctttgt tgggaatctt
     7501 aaaaaaggga tgaccgtaca agctcagttt accctggctg aacgaaaact ttggcaattg
     7561 ctttgggata aactggacga ttggttgaac ccacagtccg gaagccagtc gcctgcgaag
     7621 cccagatagc ccaaccggat cagatcgatg agcattccgg ccagggtgaa aataaggcaa
     7681 agggatatca ccgattgcgg tgcggcctgc ctggcctccg tatccgcttt ttaccatttg
     7741 aaaataccta ttgcccggat ccggcagaag gcgggcactg accaaaaagg aacgaacatc
     7801 ctgggtatga tagaagcggc ggagtatctg ggttttgatg ccaggggagt caaaggacct
     7861 ttcgaggcct tggaaaccgt acccctcccg gtaattgtcc acctggtggt ccgggaaatc
     7921 ctttaccatt attgcgtttt gtataaaata gacgcgaaat ccgttacgct gatggaccct
     7981 gcaaccggga aatttgaaaa aaagaaaatt caggaattcc gggaacaatg gacaggggtc
     8041 gcggttttga tttccccctc cgaccggttc cggaaaggca ataaaaagac cccaaacagt
     8101 ataagattct ggcaattgat agcgccggaa tccggcctga tcagcctggc gtttataggc
     8161 gccctgcttt acactctttt gggcttgtcc acctcgatct atgttcagaa aatcgtggat
     8221 tttgttctgg tcgatgggaa taccaggttg ttgaacctgc tgagtgtggc catggtttgc
     8281 ctgctcctct ttcaaatgag ttttggattt ttaaaaagct ggttttccat gaaagctggt
     8341 caacatattg accgaagact gatattgggt tattacaaac acctcttcag gctgcccaag
     8401 cggttttttg ataccatgag agtgggggaa atcataagcc gggtcaacga tgcggtcaag
     8461 atccgcagtt tcatcaatga agccgccctg ggtttgatgg tcaatcttct cattctcctc
     8521 ttttcgctgg ccgtgatgtt tgtgtattac tggaagttgg ccatactcct gctttttatc
     8581 cttcccgttt atttccttct ttatacgttg agcaactgga tcaacaaaaa gtgggtccgg
     8641 cgcatcatgg aggaatcggc ggaactggaa gcccaatggg tggaaagcct gaacgcagca
     8701 gctaccatca aaaggttcgg agtcgaaacg cagtggaatg aaagaacggc ccggaaattc
     8761 aacgctcttt tatactccgt attcagatcg ggaacatacg gcctggcttt gtccaccggg
     8821 gtcgaatgga gcaaccggat cttcacgatc ctgattcttt gggcggggtc ttattttgtg
     8881 attgcaaggg agatgtcggc gggagaattg ttttcttttt attccctcat cgcctatttc
     8941 accggtcccg ccgcatccat gatcggagcc aatcgggccg tccaggatgc cctgattgcc
     9001 gcggatcgtc tgtttgaaat cattgacctg gacaccgaac aaaatgaaga tacccggatc
     9061 tgcctgagcc cggaacttct ggatgatatc cagttcaccc gggtagggtt tcgttacggc
     9121 acccggaagg aggttttccg ggacctcaac ctggttatcc gaaaaggaaa atccacggcc
     9181 attgttgggg atagcggttg tggcaaatcg accctccttt ccctgcttca gaatctatac
     9241 ccgcttcaaa aaggaaaaat aaccattgga atttttgaac tgaatcagat gtccaatgcc
     9301 agtcttagaa aaatagttgg agtggttccc caggaaatca gtctttttga cggtaccatc
     9361 gctgaaaata tagccctggg agaattttat cctgacctcc ggaaaataac ggaaatcggt
     9421 caccgtcttg gcatcactga atttgtcgaa caacttcccg gaggatacca ttccaggatc
     9481 agcgaacagg gaaccaatct tagcagcggc caacgtcagc gcattgccat agcgagggcg
     9541 ctttaccgtg accccgaaat cctgatcctg gatgaggcaa cctcagccct tgaccccata
     9601 gccgaatcca gggtccggga aacattaaat tggtttaagg accgtgataa aaccatcatc
     9661 cttattgccc accgcctaca taccatccgt cattgcgatg aaattgtagt tcttcacgag
     9721 ggcagggtgt cggaagcggg gacacacgaa aatttaatga acttaaaggg ccgttacttc
     9781 cagatgtttt cggcataaac tccgggatca tctaacattg ttcctgaaag cgtcccagcg
     9841 ggtgaatgaa atggacaggc agggatagtc ctttcttttt ctaattttac ttccattata
     9901 aaaaatagtg aatgaaaaaa acgggaaacg ggaaaagcct ggacctcgtg gcaataatcc
     9961 tgttgctttt attgatttcc tgtgcggaaa agaagcaaaa gcggtattca gatcctctga
    10021 ccccggcaga agccatgcag cattttaaaa tagcagaggg tttccatttg gagcttttcg
    10081 ccgcagaacc ccaggtcatg tccccggtag acatggccat ggatgaacag gggattctct
    10141 atgttattga aatgggcgat tatccatata tgcccgaacc cgggtccgcc aaaggcctga
    10201 tcaaagcgtt gcaggacaaa aacaaggacg gaagggtgga tacggccatt gtatttgcaa
    10261 caaaactacc gagtgcaacc agcttgttgc cctggaaagg cggcctcctc gtggcggccg
    10321 ccccggatat tttttttatg aaagatacca ccggggattt tgtagcggat atcaaggagg
    10381 ttttatttac tggatttttt gatgataatt cggaagccca tatcaccagt ttccgttttt
    10441 gcgtggacaa ctgggtatat gcaaacaaca acggacagac aggggtgatt actgcgccag
    10501 ggcatccctc attaccagcc gtgacgatca gcggcggcga ttttcgtttc cgcctggata
    10561 cggtattgtt tgagaaagaa tccgggaccg gtcagtttgg catgaccatg gatgactggg
    10621 gcaaccgctt ttacactcag aatacctggc atatccaaca accttcgatc aagggcagat
    10681 atctttttcg tcacaagttt ttgcccgatc aaaaggcgga tgtaaatatt tcagatcatg
    10741 atctggaaat gttccagctc accccacccc cttattggag actcgaacgc agtaaccggc
    10801 gccagagaaa tttcgattct ttaaaactgg accgaaggga atgggccgaa gatcatttca
    10861 ccggggcttc cggagggact ttttacgggg gggatgccta tcccgaatct tattatgggt
    10921 ccatttttac tggcgaggtg gccggcaacc tggttcaccg ggatatcatt gctcccagga
    10981 gcgacaaacc cgtttttagc gcccggcgcg gccccctgga aaaagacagg gaatttatcg
    11041 cgtctgacga tccctggttc cggccggtta acttatggct tggacccgac ggtaaccttt
    11101 acctgattga tatgtatctg cagcatattg aaacccccct ttctattccc ccggatcttt
    11161 tggcggatat ggatttctcg gccggagata aatatggacg tatttaccgt atcgtttcgg
    11221 ataaagcacc agcccgtaaa atgcctgaat ttaatctgag gaagaacagt tctgcagaac
    11281 tcgtacaaaa tcttgctcac ccgaaccagt ggtggagact gcagtcacaa aaattgcttc
    11341 ttgaaagaca agaccaatcc gtggttccat tgctgaaaaa catgttcgcc acccatacgg
    11401 atccccgggc aaggctgcat gctttatata tgctggatgg ccttaaacaa ctgaatgccg
    11461 atctggtgag ccaggctctt aaagaccagt catggggtat aaggaaacag gcctgcctgc
    11521 ttgctgaacg ttacccttct ttatttgacc cattaaacag ccttctatcc gatacctcca
    11581 tacaagtggc ttttcaagcc gctttaagca tcggggaatt tccgccttct cggtccatgc
    11641 ccgccttgat ccgggtattg gaaaagtacg gatccgaacc cctgtttcga tcagctgtat
    11701 tgagttcttt accgggatca tccatgcctt ttcttaacct attgtttacc aaaagccaat
    11761 ggctcaggac tcagcaggaa gatagcgtag cttttatgac ggattttgca tacattatcg
    11821 gtgtccgcag gcaagccgat gaggtgaggg ccctgatcac cctcttgtcg gatactgacc
    11881 tgatgaatga gggcctgcaa aaatccgccc tggccgggct cgctatgggg attaaaagcc
    11941 taaagaataa aaataaagca gatcctggct tattttcagc agtgcaaaac ctgaaattgg
    12001 gaaaaacgga aaaattgaag tctgcggtag atcaatttat ggcggcaata agggatactt
    12061 tatagcaaaa tatttaaaca gcatattctg aatggacaat ttgatatcac gaaggctttt
    12121 tgtacggaaa tggttttttt cgggtttcct ccttggggga gcggcggccc tggccggcgg
    12181 ttgtgcttca aatgaaaaaa aatcgcccgg gcctgccgaa aaagaggccg cggcggagga
    12241 aaagtcaggg ccgccatcag gaccggaaac taatcatacg ggcgacccct gcaccgatta
    12301 ttcaggagtt tcagaagcgg acttaaaaat cagggaagcc atgggttatg tccctctttc
    12361 gaccgtggag ggaaaacaat gcagtaattg taacttgtat cttcccggaa ccgcatgtgg
    12421 aagttgccag ttgtttaaag ggccggttac agccaatgga aattgtactt attgggcgcc
    12481 gcaagtctaa gggtggatgg gataaatcaa tcatgaaatc atatttcagt tacggcttgt
    12541 tcaggataaa gatcagagac cggatctgcc tgttattggg atcagtcctg tttttttcat
    12601 taaaggccca ggaactgagg atagggacct tttatacgga tgccacgcct ccgatcggca
    12661 gcccggtagc ctatgccatc gcccgttcgg ttacggaacc cttaagcgcg aaaggcattg
    12721 tgatcctttc agatcagaaa cctatcgtgt tgtgcgcggt ggactggata ggcatatcca
    12781 acgaagggct ggaggtcttt gccaatcaac ttgcccttgc agccaatacc tccccggagc
    12841 gggtgtccat tcacgccctg catcagcacg acggggtccg gtgcgatttc accatcgaac
    12901 ggattctggc cgaatatggc ctgggcggaa ggcattttga caatgtgttt ttaaataaag
    12961 taattcaaaa tacctcggcc gctttgaaaa aggccatgca ggacaatcag ccctttaccc
    13021 atatcggttt tggccaggcc aaagtggaca gtgtggcttc caaccggagg attttgggcc
    13081 cggacggcaa ggtcgccatc attcgcttca gcaaatcggt ggattcggcc gccatccatg
    13141 cgccggaagg attggtcgat ccctggctga aaacagtgag tttctggaat ggcgaaaaaa
    13201 ccctggcggt gttgacctat tataccacgc atccccaaag ctattatggc gaaggagacg
    13261 tcacctgcga gtttgtgggc attgcccgcg aagcaaggca ggcgaaaatg aacggaatcc
    13321 cgcacattca ctttaacggg gcaagcggca atatcgccgc gggcaaatac aacgacgggt
    13381 ccaaagctag aagaccggtg cttgccgccc gggtggaaaa gggaatgacc gaagcatgga
    13441 agaataccgt taaaatgcag gtctcggccg gtatgctgga atggaagaac cttaaagtga
    13501 aactgcccct gggtaagaat atcgtggaaa gcgacctgag ggcaaggatc acgaatgatc
    13561 agaacacccc tccccagaaa tttgtagcgg ccgaaaagct ggcttggtac cagcgttcaa
    13621 aggcaggcgt aaaagtgaat atttcagtca ttaaattagg gacgatccat ctcctgaacc
    13681 tgccgggcga actttttgtg gaataccagc ttgccgccca ggaaatgaaa cccggtgaac
    13741 aggtttgtac ggcggcctat gaagaatatg gacccggata tatcggtaca aaaatttcgt
    13801 atacccaggg tggttatgaa accagcggta tcgtctcggg agtagctccc ggcgtggaaa
    13861 aagttttatt gcgggcgatc cataaagtat tgaaataagc ctcccgaaga acatggacag
    13921 aagaaccttt gtaaaaaaca ccgcggcaat ggccgcttta aatcctttca tggaaaggaa
    13981 ggaagttttt attactccca aagcaccctt ggtaaaagtc aaccataccc aatccaattt
    14041 tgagcgcgaa aaattgttgc ggccgtttgg attcaaagga ggatacctca ccgagttatg
    14101 gcaaaccgcc gttaagcttc aatcggaaaa atacagcgga atcgggctgg cgacccaaag
    14161 cgtattgtat ggcgatgccg acctgtttgt ccgatattcg gaaacgggag gcaacgcgct
    14221 catgtatgcc ttgacggaaa aagctttgca gcaggtcaaa gaaaccccct tttctgatcc
    14281 ggtcaccctg atggataaaa ttcaccccgg cctttacgaa gaagcgaaaa gaatgaccgg
    14341 taaaagggat ctcaatgaaa acttcctgat gaatgcactg gtcagcgtcg ataatgcagc
    14401 ctggttgttg tatgctgcgg agaatggcct ggaaaacttt tatgatatgc tgccggagcc
    14461 ctataaaccg gctttatcgt acaagaataa taaagtagcc tttatgttcc aggtatctta
    14521 cggcatgccg gtggaggagt tggtgaaagc cgtcgaggaa ggctatttta tcattaagat
    14581 caaaaccggc cagcccggca cccagtccga aatgctgcag aaggatatgg ccaggctcag
    14641 ccagatccac caggcgttaa aggattttcg tacgaatcat acacccgatg gtaaattgat
    14701 ctataccctg gatgccaacg cccggtatga aaaaaaggaa acccttctgc ggtatctgga
    14761 ccatgcccgg gtcatcggag cctttcccca aattcttttt attgaagaac cgctgaatga
    14821 aaacaattcg gaagacgtat ccgacaccgg ggtgcggatc ggcgccgatg aaagtgtgca
    14881 cgatgaaagg accgccctgc agcgattgca gcaaggatac cgcctgatgg tattgaaagg
    14941 aattgccaag accctgagta aaactttaaa aattgccagg ctggctcatg aacgcaatgt
    15001 gccctgcatt ttagccgatc tcacggtaaa ccctatcctg atcgattgga ataaaaacct
    15061 ggccgcccgg gttaaaccct ttccgggcat ggatatgggc atgatggaaa ccaacgggga
    15121 tgcgaattat gtccattgga aaaccatgca atccaggcac ccggccgcag gcgccccctg
    15181 gacaagggta aacaaggggg tctttgaact ggatgaagaa tatttcaacc gcagcggggg
    15241 catttttgaa aagtccacgc attacgaaga attgttaaaa ggataaacat tccatgaacc
    15301 ggcgcagcgg gtttcttttt ttattggaaa aatgtttccg ggttctggtt tccggaatat
    15361 tcctgtttct cgtccaatgt aaaccgtccg tacctcaaaa aggtttgcta acctatacgg
    15421 aagaaaacgg gaattataag ccggtgacga atccggcgga atgggaaatc aaaaggaaac
    15481 aagtccttaa gggcatggaa gaggctatgg gggccttgcc ttcacgggaa ggtcttcccc
    15541 ccatggatgt cgaatatacc gatagcctgg aagaggccgc ttatacccga tacgacattc
    15601 gattcgtcgc agcggtcaat gaaatccttc ctgcatatct ctatataccc agggacaagg
    15661 gggaaaatca aaagtttccg gcgatgcttg ccctccatga aacggattcc attggcaaaa
    15721 aaagtgtgga cggccaggga aagaataaga acctcgctta tgcaaaggaa ttggcagtca
    15781 ggggatatgt ggtgattgca ccggattatc ccggcttcgg ggatttgcag ccttatgatt
    15841 ttagttccga ccgctactgg tccggcacta tgaaggggat ttttgatcat atccgttgca
    15901 tcgacctgct ggtctcccgg cctgaagtgg attccggtcg tatcggggtc atcgggcatt
    15961 cattgggcgg gcacaatgcc ctgtttgttg gggcttttga tacaaggttg aaagcgatca
    16021 tttccagttg cggctggaca ccctttgccg attatgatat tggccccttt gccgtggaga
    16081 aatacggagg aaggcttggc ccctatgcac aggaaaggta catgcctttg ctcagggata
    16141 aataccatct ggaggatgcc ctgtttccct ttgattttac cgatatcatt gcttcattgg
    16201 ctccccgcgc ctttttctcc aactcgcctg aaagggatga caactttgac gtgcggggag
    16261 tacgtaaggg aattggggaa gcgatgaaag tataccggat gtttgtacag gaagaccggc
    16321 ttcaggtgcg ttatccggat accaaacacg atttcccgga ggaggtccgg agggaagctt
    16381 accggttttt ggattctatc ctgggtcata agggggaata attatttttg cgcccatggc
    16441 tccttcaaaa accaaaatca ttcatcactt gtccaaagac ccggtgctcc agcccgtcct
    16501 ggaaaccgtc cctttcccaa aactacaacc cagttccgta cccctcagcg aagctttgat
    16561 tgaatccatt gtctatcagc aattgtccat caaggccgcg gccaccattt accagcgttt
    16621 cctggaccgg tttgagaaca aacaacttga attaaaaaaa ctggcccgca tgaaaacgga
    16681 atcgctccgg tcggtcggct tgtcttacca gaaggcggat tatgtccgca acattgcttc
    16741 gtttttcctt cagaaagaaa accaggatat agactggccg accctgccgg aggaggaact
    16801 tcttaaaaga ctggtcacca taaaaggagt gggtcaatgg acggtccaga tgatcctgat
    16861 caacccttta gggaaactgg atgtctttcc ggccctggac ctgggtgttc agcaggggat
    16921 cgtgcgattg tatgaattga aggagacagg caaaaacctt atttcacgga tgcacgaaat
    16981 tgcggaaccc tggcgtccct accggtcggt tgcctgcatg tacttgtgga gatggaaaga
    17041 tcagtttcga aagtagggcg ccctttttcc gttttgaagg attgttcagg cctcccgggg
    17101 tttccatttg atattgcacc ctccgctggg atattgttta tccgtcaccg gctcgtggtt
    17161 taagacggca tccagggcct tgcgtaaatc tgtccccgta agaggaacat catttttggg
    17221 tcgcgatccg tcaagccgtc cccggtaaac cagtttcagc tcgtcgtcaa acaaataaaa
    17281 atccggagtg catgccgcgt cataggtccg ggcaacctcc tgggattcat cataaaggta
    17341 agggaatgga tatccctgtt cggcggcagt gatcttcatt tgatccggac catctgccgg
    17401 gtatttttgg gcatcgttgg aactgatggc gataaatgaa actcccctgg gaatatactc
    17461 cttcgcaatg cggacgattt ccggattcac atgttttaca taagggcaat gattgcatat
    17521 aaacatgatg agggtagccc ggccggattt aagttcatcc agggaaaagg atttcccgct
    17581 gatcacatcg ggtaaataaa attcgggggc cctggtgccc agggggagca tggtggattc
    17641 ggtgtaggac atctgtcgct tagaatggga taaattaaaa aacaaaatta atgactaatt
    17701 tgacccgggt tgaaatgaaa atacttcttc tgcaatgatc accctcatgc ggaccacccc
    17761 cggtcatccc gaattcagaa gattggtgca ccagctggat gaaaccttaa aggaactcga
    17821 tggagaagac catgtgattt atgctccgtt gaaccaaatc catgtgctta aacatgccgt
    17881 tctggcaatg gaaggcgatg aagccttggc atgcggggcg tttaaacatt attccaggga
    17941 actggctgaa atcaaaagaa tgtttgtggt ttcttcaaaa cggggagagg gattgggagc
    18001 gctggtgctg aatgaactgg agtcctgggc caaagaactc ggccattcgg cctgcatcct
    18061 ggaaaccggc cggaacaatc ctatcgccat ccgcttgtat ctgaaatccg gttatgaaga
    18121 aattcccaat tacggacctt atgagaaact gatgtccagt atttgtatga gaaaaaacct
    18181 ttgaaattca gcactaacct ttctctcccc ccaccggacc gtaaaagatc acccaggtgg
    18241 aaaaatcatc ggtaaaatcc ataaaccggt gctcctgccc agccggcaca aacaggaagt
    18301 catgagggcc aaacaaaact tcctggtcgc cgttaataaa acgaccctga cctgaaataa
    18361 taatataaac ctcatcgcgg tcatggggtt tttgatgatc ttcctttacc ggcttataga
    18421 tttccaccct caggctgcca tgttcaaaca aggattcaaa caagccgccc gtaccggata
    18481 atctgctttg agccgtctct aaggaaatat gcatggtttg atcaacatta attcatcttt
    18541 tcaatcccaa cgaacgtttt ttgtctgcat ccgtaaaaat attttgaaac cgattacatt
    18601 ttacgtctta ttccttaatt ttagcatgaa ataccggata aaatgacaaa aaaattcaga
    18661 tcaggaagaa ggcaatttat caaacagggc cttcagacct ctttcgccct cagcctcccc
    18721 acgattgttc ccgcaagggt atttggccgt tttgcgccca gcaaccgcat taccgtcggc
    18781 gccattggca acggccggat ctcccgcacc cacgatatgc ccggggtctg gaggcaggac
    18841 tatgcgcaga tcatcgcggt atgtgacctg gacacccacc gtgcacagga cggtaagaaa
    18901 ctggtcaacg aatattattc caaaaaatac ggaaaaccct acgacggggt tacggctcac
    18961 tatcattacg aagagttgct gcaaaacaag gacatcgatg ccgtcctcat cagcacgccg
    19021 gaccattggc atgcgcgcat tgcgatggat gccgccaaag ccgggaaaca catctacatg
    19081 caaaaaccgg catccctgac catctcggaa gggcgcaagg tcagtgatgc ggtccaaaaa
    19141 agcggggttg ttttccagat cggcagtcaa caacggtcct ccacccagtt tcgttatgcg
    19201 gctgaattgg ttcgcaatgg aaggataggc cagttgaaaa ccgtttcggt cggtttgccc
    19261 ggggatcctt ccggggacat ggagcccgaa atgcccgtcc cccaaaattt aaactacgag
    19321 atgtggctgg gcagcacccc cgaggtatat tataccgaaa aaagggttca tccccaaaat
    19381 ggttatgatc gtccgggctg gcttcgctgt gaacaattcg gggcaggcat gatcaccggc
    19441 tggggctccc atcatatcga ctcggcacac tggggtatgg gcactgagcg caccggcccg
    19501 attgaaatct ggggccatgc cgaatttccg aaatccggcc tgtgggacgt acatggtatt
    19561 tttcgcaccg aggcccgtta tgccaacggg gtgcacatga tcgtcagcaa tgaactgccc
    19621 aacggggtca aatttgaagg cacggaaggc tggatttttg taacccgcgg caattaccgc
    19681 gccaccgctt ccgatcccgt ggtcaatgcg gacggcacca aacccctgga tgccagcgac
    19741 cccaagatcc tgacttcgga aatcggcccc aatgaatttc attttatagt cagcagcaac
    19801 catcacgcca actggcttga ggccatacgg gataaaaaac agcccatagc gccggtggaa
    19861 gaagcccacc gctcctgttc agcctgcctc cttcaccaca tcgccatgaa actcgaccgg
    19921 aaggtccatt gggatccggt taaggaagac ttcgtcaatg atccccaggc ctccgccctg
    19981 ctctccaggc cgcagcggaa accttatggt atttaggaat cagggattgc agcaagggtg
    20041 aacagccttg ggattttatc tttttcagaa acggcggctg gattcattga ccatagcctg
    20101 gataaaagcc ggtggaccca tgaagcccat atcactacgg ccatttggtt cctgttgaac
    20161 tatgaaaaac aagaggccct ctgccggctc cgctcgggca tcatttcata caaccttgcg
    20221 accggagggg aaaacacggg atcaagcgga taccatgaaa ccatcaccgt cttttggtgg
    20281 accctgctgg aaaggtttgt gcaggcaaat ccgggcaaag actatgatca attgtgccga
    20341 gcctttttaa attcccggta tgcccatcgc gaaacaccct ttgaatttta ttccagggaa
    20401 aagttgttgt ccgcccgggc ccgggcctat tatcttgcac cggatattaa agaaatccat
    20461 ctctgatgaa aaataacctt ttcctgctgt gctgcagttt tatctttatg cattgctcag
    20521 cgccgatgcc ggtaaacccc aacccttacc agatcataac ccttgatccc ggacattttc
    20581 atgcggccct ggttcagaaa tccatgtacc ccggggtgga ttccaccgtc cgggtctatg
    20641 cacccggggg accggacctg gatatgcatt tgcaaaggat aaaaaattac aacgaaagaa
    20701 aagaccagcc tacccggtgg aatgaagtgg tttacacggg accggatttt tttgaacgaa
    20761 tgatcgcgga caaaaaaggc aacctggtgg ttttatccgg caacaacagg ctcaaaccgg
    20821 attatatcct gggttccctg gaggccggga tgcatgtcct ggcagataaa cccatgatca
    20881 ttcaatatga ggattttgaa aaactgaaaa cggcctttga actggctaaa cagaaaaata
    20941 tattgctcta tgatatcatg acggagcggg atgaggtttc aacattgatg caacgcacgc
    21001 tttccatgaa taaggacctg ttcggagaac tggaaaaagg cagcctggaa caaccggcga
    21061 tcaccaaaga gagtgtacat catttgtaca aaaccgtctc cggcaatgtc ctggtccggc
    21121 caagctggtt tctcgatgtc cggcaacagg gagaaggact ggtggacgtg atgacgcacc
    21181 tggtggacct cgttcagtgg gaatgtttcc cggaccagat cattgatacc caggatatcc
    21241 gcattctgaa agcaaggcgg tggactaccg acctgaacct tgaaaaattc accgccatta
    21301 cccaatctcc ggctttcccg gattttcttc accaggacct tgtacaggat accctgctca
    21361 aagtgtattg caatggagaa atcaactaca ccataaaggg gatccatgcc aaaacttccg
    21421 tgatctggaa ttataaggcg ccggaaggca gcggggatac ccactattcc atcatgaggg
    21481 gcaccaaaag cgacctcatc atccggcaag gggcaaatga aaattatcag cccaccttgt
    21541 atctggaacc gagaagaaaa ggggatacca gcattgccct tgcattaaag gagcaaatta
    21601 agaatcttga tttcgatggg cttggtattc aatttattcc tactgaacac ggatggaggg
    21661 tttcctttcc ggccaacatg ctggagggtc acgaagccca ttttgcacgg gtcatggaaa
    21721 aattcctttc ttacctgaaa gataagaata tgcccgcctg ggaagtgccc aatatgctgg
    21781 ccaaatatta tacgacaaca aaagccctgg aatgggccat gaaggacaat gggctgtagt
    21841 gggaaaagca gccccttaat tccttaatta ttaattctta attgtaatta tgaagtcaac
    21901 ctccctttaa gccctgctct gttgtccttg ttattcggat ttttttccga catgctatac
    21961 aggaaattta catggatttc atgttcctga tcagatccca gggtcaaaag gtccccatga
    22021 atgccttcgg tatacttcaa cctgattttt aaactgccat tcggaatgca tccagcggtg
    22081 gcggctaccc tggacccgat gggcgcctga ttgggattgt tgaatggatt ttgtatccgg
    22141 tggtcattga cctgccattg attccaggca aaagggattt ccactttgcc gcggctccca
    22201 tccagcagga tatgcccgcc tttgttgcca gcgttgacct ttatcgattt ccaggaccag
    22261 ggattttctg caagttgtat ttctttgccg ttgatccggt ttaccagggg gcttccattg
    22321 gaacccagtt gggaagtgag ggaaaggcta ttgctcaagg cgctcaattc ctcgtatttg
    22381 gatttattct cagccaaggc ctccgctttc atcccgggca gcaggtgatc ccacatcaag
    22441 gtcatttgtt tctgcatgtc cgagctttcc gaattgaggg cgatcaccgc atcatattgc
    22501 ggcatgacta cgcaatattg tccaaaagcg ccatcgcccc ggtaaaatcc gggtttacag
    22561 cgccagaatt gataaccata accctgggac cagtccccat cgccgggatt ggaagctata
    22621 tgagaactgg tggcttcatc cacccatgcc tcccccagca gtgctttgcc ttcccatttg
    22681 cctttctgta aatagaattg gccaaaacgg gcgatatcgt ctgcggtaac ccggagaccg
    22741 taaccgccgg tatttatgcc ttcggggctc acttcccaat cggattgagt gatccccagc
    22801 ggccggtaaa gcctgggacc caggtattcc tgaaggcttt gtccggtaac cctggaaata
    22861 atggcgctgc agatataagt cgcccccgta ttataaagaa aatgggtgcc gggttctttt
    22921 tcaacgggac aagccaggaa agatttgatc caggaaacgc cctgcgcggc ccgcatgacc
    22981 gggatggtat ccttgatatg gcccgtggac atgttcaacg catggcggac ttccatcctg
    23041 gacaggtttt cagaaaccgt ttcaggcagg tgttcaggga aaaaagaaat taccttgtcg
    23101 gtgagcctga tctttccttc ctgtacggca aaaccgatgg cggtgctggt aaaactcttg
    23161 ctcagggaat acagggtgtg tacctggttg cgctcgaagg gtttccaata ggcctcggca
    23221 atgactttgc catgcctcat caggataaaa ccatgatgct ccactccgct tttatcagcc
    23281 tccgtaagat attggatgat cgattcggaa aggacccctg tactttcagg cgtggccctc
    23341 ggtaagtccg gaaccggatt catggataaa aaagaaaacg cgggagggaa cagggaccct
    23401 gcggaaagaa gggatccgta ttgtaaaaac tgcctgcggc caaggtccat catccgtatt
    23461 gttttttatg aaatggtaaa ttatatattg ctgcggatac tttgttggtc taagctttcc
    23521 tattttgcgc cgactgttag aaaaacggat tgaataaaac aattttatct tcgattgttc
    23581 attttcttta agagttaacc tgttcatgaa aagaattgtc ctgctcctgt ttgtccttga
    23641 aaccaccttt aacctttttg gccaatcgac gcccgtcgtc tccggtccca tgcttggtcc
    23701 ggtggagctt agagatgccc tgatctggct ggaagtgagc ccttcggtcc gcacggtttc
    23761 ccttcaatac cgcaaaaccg gtaccggtgt ttataaaacc aaggcctacc gcggcccatt
    23821 ggggaatgaa ttcaatccca ttcaaatcca ggtcggcgga ctcgatttga atacggaata
    23881 cgaataccgc tttaacctcg acggcaagcc ttcggctgca tccggtaaat tcaggaccaa
    23941 ggacctatgg caatggcgca agccggctcc tgatttttct ttcctcaccg gaagctgcgc
    24001 ctatgtcaat gagccggcct ttgaccggcc gggcaggccc tatggcggcg attctaccat
    24061 attcgaaacc atggcccggg aacaggccgt tttcatgctg tggaccggag atacctggta
    24121 cacccgcgaa gtggattatt acagtaaatg gggcctttgg taccgcgccc accacgaccg
    24181 ggcggtaccg gtgcttcagc cgtttttgaa agccatgccg cattgggcca gctgggacga
    24241 tcacgattac ggacccaacg atatcggatc gaattatatc ctgaaggaag aaagccggaa
    24301 ggtgtttatg cattatttct gcaatccttc ttatggggaa aacaaccagg gcatttacac
    24361 catgaacagt tggggggacg tggatatttt cctcctggac gaccgctggt ggagaagcgc
    24421 ggaccagctc ccagattcca tccatggacg gcccaatccg gataaaatag tgattgggtc
    24481 ccagcagatg aactggctca aaaacagcct ggtctatagt caggccactt ttaaaatcat
    24541 tgttttgggc agccaggttc tcaacccggt gtcggctttt gacaaattcc ttgattttcc
    24601 cgtggaatac caggagctga tggattttct catgcagcat aaaatagacg gggtgctttt
    24661 tctttccggg gaccgtcatc attccgaaat cattcgcgtg gaacggcccg gagcttaccc
    24721 cttgtatgat atcaccgtat ctcctttgac ttcggggacc catcgttttg ctccgaatga
    24781 gcagaacaat ccctaccggg tcctcggggt ggatcaaaaa cagaattatg gccggtttac
    24841 gatttcaggc ccgcgccatg agcgggtgat gaaagtggaa ctcctgggcg tgaaagggga
    24901 tcttctggcc gactggcaag tccatgaaaa ggaacttaaa ttccaaaaat aagcgcacaa
    24961 ttttttaaag aacaaggctc aaagctcctg ccgttttaat gatctgactg cagggggatg
    25021 ccgaaattga attgatccaa tgaaaaaaaa atggttactc tatgcctttt tctctgccct
    25081 gatccatgtt tttcaggcct gtgataataa ccagttccct ccggaaacca tagaagtgag
    25141 gggcctgttt tcattgacgg gcaactggtc ctcgctgggc attgcttccc aggccgccat
    25201 tgatcttgcg gcggaggata tcaatgatta cctggaagac aggaaatccg gttttaaatt
    25261 gtcggtttcc gtggccgata ccaaactgga taccgggctt gcaaaaacat tattcacctc
    25321 agcccaagag gataaaatgg gttttatcat cggaccgcag tccagtgcag aactttcggc
    25381 catcaaacca ttgagcgatg cggcggaaat catcgtgatc agccaaagca gcacagcagg
    25441 tagcctggca attgccggag acgctatttt tcgtttttgc ccttcggaca aaatcgaggg
    25501 ggcggccatg gcggcaacca ttgccaaaag gggattcaag ggattggtca ctgtggccag
    25561 ggatgatgcc ggaaacaagg gcctgcagac ttcaaccggt gcggccttta cagagaaggg
    25621 cggacaggtt gccgcgattc caccctatgc ggccacgctt acggattacc gtaccgtagt
    25681 agcttcgata aaggaaaaac tcggtacgtt gcttgctgtg catggaatgg ataaagtcgg
    25741 agtctatctg gcatcctttg atgaaggaac tgaaattttc aggcttgccg cgatggaccc
    25801 ggttttgagc aatatcaaat ggtttggagg agatggcgcg gtgctgagca cggttttttt
    25861 aaacgatgcg gaggtggccg actttgcaat aaaaacctct ttttttgccc cttcctttgg
    25921 attgcccgtg gcaaacgaag cggattggaa accggttgcg gcccgcatta aggcaaggac
    25981 cggcaccgaa cccgacgctt ttgccattgc ggcctatgat gccttgtgga ccatagctta
    26041 taccatcgaa gccaatcagg gaactacgaa aggcttttcc aatttaaaaa accattttgt
    26101 agaacaggcc aatagccgca gcggcatcat tggtgcgagg gccctggatg aagcggggga
    26161 ccgggccaat ggaacctatg actattttgg tattgcaaag gaaggcaaca cctatgtctg
    26221 gaaactcgtg ggaaaatcta cggattaagg ctaactttct ctgcggaatg accctgaaaa
    26281 aatcatgata tcgtcatgga tcttttccct ggccggatcg ctgatctttc caattttgat
    26341 cggttgcaac ggccagactc aaaacaagat taatggatca gaggcaaatg taaggcattc
    26401 agatggaatc ggctgggggt tccatgactg tttatcggat gcgtttgaac atttctttag
    26461 cgatttgatt gaacctggtc gggtcaggaa gattgcaaat acaaagcgaa aaacaagagg
    26521 cagaatcctt aaactgggca ggccatgata ccagggatcc cttattttcc tgaagttgat
    26581 accaaatgat acgcaagctc ttttatcaac tttcccccct gctccggttt ctggtcaggt
    26641 atgtggccta tctgccggct gacctgatct tggggcggaa aaacaaaatc ctgcctccaa
    26701 gacgactgat ctataccggg cgcggcgatt ttgaaaggca aggccgtgag tggctggaat
    26761 tctttatccg ggaggcgggc ctgcaacctt cctcctccgt cctggatatc ggaagcggga
    26821 ttggccgcat cgccataccc atgatggatt ggctgaaagg tccttatacc ggctttgacg
    26881 cggtgaaagc aggagtgaac tggtgcagga aaaacatcag cagccgcaga ccgaatttct
    26941 tttttcaatt tgtggacctt cgaaatgatc tttacaggca caagggccgg aatgccgccc
    27001 attttgattt tccttttgaa ccaaagtcct ttgattttat ctgcgccatt tccgtgttta
    27061 cccacttatt gcccgaagaa acggaaaact acttcaggca aatcaggaaa gtgataaaac
    27121 ccggaggtta tgcggtgctt actttctttt taacggatga ggagacccac cgcctgatgg
    27181 caaaaaacaa agacggcttg ttgtttttat accgtttcgg ccattattcg ctgatgaatc
    27241 tctccgtgcc ctcggcaaat gtggcctatc agcgggaata catctatgcc ctcgcggaat
    27301 caaacgatct gaaaataatc aaagaaatca aaggctcctg gtgcggaagg gaaaaaagtc
    27361 attacctgga tttccaggat atcttggtgt tgcataaacc tgccggagct taatagggtt
    27421 ttaagttaaa ccggcagggt ccagttagtc tttgtttttc agcttgtatt tttctttgaa
    27481 cttttctttc tccttgggat cgttcaggtc gtaagtttca atatccccgt ttttgtattt
    27541 gattttgact ttatcgtgat tgatattaaa atgtgtgacc tcgtccggta agtcccattc
    27601 aattacagga gggaccggag gaaccggagg tgcgggggga acgggtggcg ccggaggaac
    27661 cggaggaaca gttggtactg gaggtaccgg gggtacgggg ggagcatatc cgttttcatt
    27721 gatgggaggc attgggggta tcggaggaac gggaggaatg ggtggaacag gaggtactgg
    27781 tggtgccggg gctacgggag gggccggggg cgccgggggg attattagtt ctccgtattt
    27841 tctctgaaat tcaagcgctt gttcttcatc gtttagatta taattttcat tggtgccgtt
    27901 cttcaggtga acttctgcat ttttattttc aacccggatt gaccggattc ggctgttatc
    27961 gccaaatcca ttgtaagaga aagaggatcg ctgtacactg ctttttccgg gaaccgtatc
    28021 cttcggtact tccgccggtt gatcagcaaa gggcctgatg atcaccggag cctcctcctg
    28081 gatggttctg aatgcaaaca aggccagggc catcgccgga aggaacaata aaaaccggat
    28141 gcgctgcaat ctgtgggatt tacgactatt catcataatt attctgtttt ttaaggattg
    28201 aaaattgaag gcggaagcaa aggaaaaacg cggggtaccc gtagcctgaa gcagtaagta
    28261 ttggtaagct ttggcatcga ccccaaggcg caacatggcg tcgtcggtga ggtattccag
    28321 gttttcccgg atggctcttt tcaaagacca ggcggccggg ttgtaccagt tgatgatggt
    28381 gatccattcg gccagcatca tgtccaggct atgtctttgc ttgacatgga taaattcatg
    28441 gagcaggatt ttttcataat cctttccccg gaacgcatcg gaatcaataa agatgtcctt
    28501 gccaaaagaa aagggggcat ggggggcttg cagttgatga atgcggactc ccgccatgtg
    28561 aaccggcctg gtattttgtt ttatccgacg gaatgaaagg attcgaatga tcaggcgggc
    28621 ggccaggaac aacattccgg caaaccaggc ccaaaacaac cactggaacg aaggattccc
    28681 ggccggcgtc aactcccgga ttccataggg attgaccggg gtggacaggg ccggtatggt
    28741 atttgacaat tcctgcaaga caggccctga accggaaacg ctcatctcga aggaaacaaa
    28801 ggggagtacc agggatagga ccaggccggt cagaaaatag aaccggatct ccttataaaa
    28861 ggtgagcttc ctgagaaaaa gcaggtaaaa tccgtgaaac aggatgagca ggacggtcat
    28921 cctgcatagg acgaggatcg gttcggcgct catgactttt tacctttttc aatcaaggaa
    28981 atgatttctt tgagctcttt cggactgagg gcctgctgtt caacaaaaaa attgaccaca
    29041 tccttataac tgttgtcaaa ataatccctg accatttggt tgagattggc ctttttatat
    29101 tccgcttcgg agatcagggg tttgtagagg ctgacattcc cgattttccg gctggataca
    29161 aaatttttct tttccaggtt tttaatggtg gatgccaggg ttgtgtaggg aacctggtcc
    29221 ccggccaagg catcgaggat ggcccggata tgggtttcgc ccagttgcca gatcactttc
    29281 atggcttgtt cttccggagg acttaattta tgcatggtca atcaaataaa aatcaaatct
    29341 acgacatttt cgtagatgtg tcaatgatgt cgtaaattat tcggccaatc aacgttccgg
    29401 atcgaccaaa gtgggtctgt ccccttttct ccctttctcc agcctcccgg cttccggctt
    29461 actgaatttt cggtatgtta gtgccaaaat cgcatcgata tgaccgggtg gagcctaaaa
    29521 cagatcatga ccgtatttat tgtcctcttt accttggtga tgttaagtca tggactgcac
    29581 ctgctcaatc cgggcatcct gcatccccgc ttcctgacca ctttgcgctg gcttaccatc
    29641 ggggccctgg tctggattgg cctcaaaaaa aacaacctga ccacctggat ctttttatcc
    29701 atggtcatcg gcgcggagat tggccacgac taccccgggg ttggcgccaa tctcaaggtg
    29761 atcagtgata tatttctccg gatgatcaag accattattg cgcccttgtt gttttccacc
    29821 ctggtggtcg ggatcgccgg tcatgccgat atgcggcagg tgggccggat gggctggaaa
    29881 tccctggttt attttgaagt cgtcacgacc atcgctctgg tcatcggcct gctggcgatc
    29941 aatatttcca aagcgggggt tgggattgac ctgagtgcag cggatgccca gaccgtgcag
    30001 gtggccaaac aaagctggca ggatatcgtc ttgcatactt ttcctgaaaa catcgccaaa
    30061 gcggtcgccg aaggccaggt tttgcagatc gtagtattca gtatcctgtt tggcttgggg
    30121 ctttccatgg tcaaaagcgg ccgccataaa gaaaatatgc tgcaatggtg cgagagcctg
    30181 tctgaggtga tgttcaaatt cacccacctg gtgatgtacc tggcacccct ggccgtcggc
    30241 gccgccatcg catataccat cggaaaaatg ggttttgacg tattgcggaa cctgtttttg
    30301 cttctactca ccctgtacgc agccctggtc gtcttcgttc tgggggtcct tttgcccata
    30361 gccctttaca tcaagctacc cattaagaaa ttcctgaacg ccgtgtctga acccgtactc
    30421 ctggcctttg ccaccgcgag ctccgaatcc gcgcttcccc atgccatgaa agcccttgaa
    30481 ggctttggca ttccgcgtaa ggtcgtctcc tttgtgctgc ctacgggatt cagttttaac
    30541 ctggatggca gcacccttta cctgagcctg gccctggtgt ttgttgcaca ggctgcaaaa
    30601 gtggatctca gcataggcga acaaatcgtg atgatgctta ccctgatgtt gtccagtaaa
    30661 ggggtcgccg gcgtagcgcg ggcctccctg gtcatattgc tggggacggc ggtttcattc
    30721 aacctgccca cagaacccat ttttattatt ctgggtattg acgccctgat ggacatggcc
    30781 cgtaccgcca ctaatctgtt gggcaattgc ctggccaccg ccgtcattgc caaatgggag
    30841 ggcgaggtgg attttgaaaa tttgccggag ccggtggctt gaggaagagg ggtcaacggt
    30901 atcctccggc cggtttaatg ccgcgcgctc aattctgatt caacaacaag gccagttttt
    30961 ggggaagatg tatggtcagt tcattctccc ggatgggctt ttgagggaat ggctcaaaac
    31021 gccctttccg gtaccagtaa aaatcggtac tgatcccccc gggggcatcc tgaaagatcc
    31081 ttaaaatttc cgggaacaac aaggccatca attgcaagac ctgcgggcct tcgatgcaat
    31141 gaaccagcgc tatgtttttg gccgggatgg ccacgaggca acccaggttg cctgcggata
    31201 agcggagatt tttttccagt tcaatggcaa aagccgccga aaaattggca ttaaataaaa
    31261 gatatagggg aaagcggtcg cccaaaagat attccctcgt ttgtacttct tcgcgtgaga
    31321 tatggcttaa ggcgatgtga aaaaggtctt cttcggcaat ttcccattcg gcgatccggt
    31381 cccgccggat gtaattgaac tggtggttga aatcaaaaac gagataggta tgtgttccgg
    31441 gaaagtccac gcggtgcaca tattctcccg gattgggcgt ggtgtcgacc ggccggacca
    31501 gtactttcaa atactgtttg gcatggtcaa aatccttgta gaaataatca tacgcctcgg
    31561 gttgaatgcg cagttgccgg aaatgtcttt ctatttcctt tgccctgtct tctgcggaca
    31621 ggggggcaat ctttttgacc agattgtcca ggtaacaatg cgcttcctgc tccctgaaga
    31681 ggaccacgat gtctccttct tcaatccttt gtacgggata ttccagtttt tgaagacagt
    31741 ccagggcgat ccggagaatc tggttgtaat cttgttcgct gaggaagacc ttgtaacggt
    31801 caggcatagg gaaatcatcc cttttgataa aatcgggtgt attcatagtg ttgtgttttt
    31861 actaaatcct gcttccgctt atcccgggtg atgaataaag cggaatactc aaaaatataa
    31921 aaaacccgtt aaaaactata ccttaaaaaa tatgcctttc cggtataaaa aataacacaa
    31981 accccattcc agggcaaaaa tgcaaagcga agaaaggatg gccgataacc attgaggggt
    32041 ggaaatcagt gcgagcaatc ccccggaaat ggcggtgaca tatccgttga accatcgcga
    32101 acctacgatt tcaaaaaaca ggtaaataaa aatactgttc atgcccacca ccgtaaaaaa
    32161 ccagacgtat tttttatggt cccggatatc aacccaccag tacacggcag ccatggccag
    32221 caggcaccag cccagggaag caagggtaaa ggaggaggtg gctatccgct taatgatcgg
    32281 cgtgacgccg gccaggtcca gaaaataacc ggcggccagg gcaatcaaag cccagagaag
    32341 gaggcctgaa agggcgtctt ttttatattt tatcagccag attccggtaa gcgcccctcc
    32401 tatggtatgt acggaagtcg gaatgcagtt gatggctacc caaccccctt tgttgatttt
    32461 attcatcagc accaggtcca tataattccc aaaatttcgc tggtcggtaa agggctggtc
    32521 aaaaccgggg acctgggtgt acctatacag aatttcggta agcaaaagaa taccggcgca
    32581 agccaggatc tgatatgcgg caggccatcg gaagatcaaa aaagcgacca gggtggtaaa
    32641 ggacaactgg gtcagcacat cccagagttc aaacgaaagt ccgtgaggcc tgacggcata
    32701 atccaggact ccccagaaaa aaagccagcc gcagcgtttg aatattttca ggaaggattc
    32761 gttccagctc atgccgtttt cttgctgctt gtggagggaa taggccatag ctaccccggc
    32821 catgtacatg aatcccggtt ggatcaggtc ccagaatctc aggccattcc aggtatggtg
    32881 gaaaaactgg tccagggcct gatgtaaaaa cccgcccggc acggtggctt cctgcaaatg
    32941 ctcataaaga cgggtgcttt ccagggccag gagcaccatg atcaggcccc ggagaaaatc
    33001 aagggataaa agccgttgtc ccggcaaatg gtatttgtcc atgatcgcag gtaagggtgg
    33061 ttttgcctta aagataattt aagatcccca ccctccggac cgggtttttc atatatgaat
    33121 ctttctgtcg gaaaatctgt tggacgtaca aacctccggt ggatccaaac cgtctgaaag
    33181 gatatgaaaa actttggttt attactgatt ttgaccctgg ccctggcttg tgagcgggcg
    33241 gcgggacccc tgaatggggt ttgttttacc ctgacggaaa gacagtgtgc gatggatccc
    33301 ttcaattctt atgtgaagga taccaaaacc ctggaggaac gcgccaatgg ggtagcggct
    33361 tatctccgcg accaggggat cgctttgtcg gaagtaaaat ccaatcccaa atttaccggt
    33421 gcggtttgtc tggcctgtac ctgtcccagt gagtacagct accggttggt tatttcctcg
    33481 gaagacacgg taaaattgaa aaccctggat ttggccctga cagaaaagga ttgcgactga
    33541 atgattaatt ccatcccgaa gtttacgttc ggaagagttc aatcttgaag gaaaaccatt
    33601 actcccggcg tgccctctcc catcaaaaat tcttcactga tggcaaccct tgagggatcg
    33661 acggatttat aaaccaggta ttcctttatt cccaaggcac gctgaaggca aaaccccgcc
    33721 ggcttggtgg aaaattcctg ggagttgcat acccctgaaa ggcggatctt cttctcagga
    33781 ttccggttaa gaaattcgat caatttctgc atttcagcaa aggcattttg aagcaaggcc
    33841 agttggcctg gtatgaaatc aatggtataa accgcctggg atttgcggtc atggctggtt
    33901 attgaaagac cggaattttg agatggtttt tttgaaggcc tggaaacctc atccggtgaa
    33961 tccctcctgt tccggatggg gggatctgcc accggattct gttttgtctt cgtgcgcatt
    34021 tccggcaagg atctcgagac cgtcatgggc agaggttcga ttggaggatt tgcctgggcc
    34081 agcggcacgg aagcttttct gatcaccgga tccgggaggt ctttatactg gaccttcgat
    34141 ggagcgccgc ttcgggttac aatcacttcg acccgccggt tttgggccct gttttcttct
    34201 gtgtcattgg gagctattgg ccgcgtcccg ccgtatcccg ctgtagaaac cctgcccgac
    34261 atgattcctt ttcccaccag gtattttttt atcgcattgg atctgtttct ggataagtca
    34321 aggagttcat aaaattttcc aacactgtcc gtatggcctt cgatgcgtat ttgcatcccg
    34381 ggattttggt ttaaaagagc ggccagtccg tccaggctct caaacgacga aggggatact
    34441 accggagtgc ctttttcaaa atagatattt ttaagttcaa ttttttgctc cttttccaag
    34501 ggaaacatca aaaagtcgat atcatagatc ctgttatcaa actgatcgag gaggggaata
    34561 tcgatcagga ggcctttaga tgcaaatcct tctttatagg gcgtcaaccg gtatttttct
    34621 tttttatcaa tggaaatctc gaataaacca tccggcgatt taaaagtgtt ttcatgtttt
    34681 ttcatccggc tggggccata ggtgaggttc gaagccactg cttcccccgt caggctgttt
    34741 atcattcttc ccctgaaaac catgggaggt gaaaggaaag ggatttcagg gcgaaaatcc
    34801 gggctttttc cggcaaagtc cgccacaaaa atatcgctgg taccatcccg cctcgaggtc
    34861 aggtagagtc tggtgtaggg gtccatcaaa acggggttgc tgtcatcgaa tatggaattg
    34921 atagggggat tcaaaagctc cggggcactc cattgggtcc aggaaaaatc aaggcgcctg
    34981 ctcatgtaaa tatccatgcc gcctggtccg ccggggcggt tgctggcaaa aaaaagggtg
    35041 gttttatcat ccgtgatgaa tggagtgatc tcccggaagg gggtgttgat cactgaaccc
    35101 aagttcagag gctcactcca caggttgttc ttgaccctaa agcacacata cagatcgttg
    35161 tctccccggc cgtcatttct gttgaccgac agaagaatca catcaccgtc ccgggtcata
    35221 caaagattga cttccgggga attggaataa tgatcacgga tatggatggg ctccggaaag
    35281 gcaaaagccc cgtccacctg cctttcaatc accgaaaatc ctttatacaa gcttccgtca
    35341 cggccaaatt gattgatgac aatcatcctg ttttcatccg gggaaaggct gcatacgctg
    35401 ttgggcaggg cattatttat cggaaaaccg ggatggtaaa cctgatcaaa gagctgatcc
    35461 cggctttctg caatccagat gtcctgattg atggaagcgg aaacagggga ttcaatgcgc
    35521 ctgccggcga tctgggaata gatttctttc aggacgacca ggtaagtgct gtccgtttcc
    35581 gttttcgaaa gatcgatttc ctcctggatc agggtgcggt taaaatccgc gctgccgaca
    35641 cgggtgaaat aaagaatttg accgtcccgg ctgattaccg gggaaatttc atcataagca
    35701 tccgaattga tggagggccc cagtttttct atcccttgct gggcaggcag ggtccaagca
    35761 tggagaaata gtaaaaacca cgctaatttg ccccctcgtt tcatgtttat tcctgttttt
    35821 ccacgacccg tcctcactcc ccttactaac ggttttttag ttataaaatt attaaggtat
    35881 aaagggaggc aaactttaag gcaaaggtcg gtggttttaa tgaaagtgcg aaaagggtta
    35941 ttaatcataa gcttcctggc gtatgcgtgg tacgcagtgg gccaaaacgg tgcggacccc
    36001 gtaacggagg aaaaaagggt gatcaatggc caggtggtta aattgatgat ttccggcacg
    36061 gacaccttgt atatcgccga tcttcaggat gtccatatct cctctccccg ccagtttaag
    36121 aattatgaag aataccggat gtatatgcgt tacaaaaggt atgccgcgga tgtttatccc
    36181 tatgccaagg aagccatcga agtataccgg cagattaagg aggagaccga agacctgaaa
    36241 aggggaaagc gtaaaaagta catccggagc gtcaacaaag aatataaaaa cgactacaag
    36301 gagacgttca aaaacatgac ccgcctgcag ggcaaggtga tgatcaaaat gattgaaaga
    36361 gaactggatg tatccttttt tgacctgatc aaagaggtca gggggggatt cacggccacc
    36421 tattgggata cgttcagccg gttatacggc tatcgcctca gggacaaata tgaaaaggga
    36481 gaggaaccca tactggatgc cgtgctgcag gattttaata tcactgcaaa actcaagtag
    36541 gggcgactta attcgcccta cttgagtgtt cttaaagttt atttggatta aaataagagg
    36601 agaaaaacgg cgaccgaatt cacccgtcat ttgatcacat caaacattct tgaatatttc
    36661 agggtaaaac tgcggttgtc aaaacggtcc aggccccggt tttcgttaaa gaccaggaaa
    36721 aggccggtat tggactgctg cagccagccg agacgcatat tcatggacca tttgtccagc
    36781 acgtcgttaa actggatcag gctttgaaca taaaggcggg gggtaaatga ataggaaatt
    36841 ctggaacgga aaatattggt attaaagtcg ccgtagggca gccgcaccac gttgttgttg
    36901 atgccccatt ccgaggtaaa cttgtcaccc aatcttacgt taaccgagcc cgtggtgctc
    36961 gtgcgccgtc cgccaaaaaa accaccgatt atcgaccgga tgttcacgga aacattctta
    37021 ctggtgttgg acataaaaac cagttgggct tcgtggtggt tataggttcc ggaagggact
    37081 gtaataccgg aagaaatttc gaaaggcaca aagaccccct cggtggtaaa gttgatgccg
    37141 gtatggattt caagaccgct ttcccattcc caatgattgt caatatgcag gaagccggtt
    37201 tccagtttct tgttcccgta attccagtag ctgcggtagg aaatatgggg gcgcaattcc
    37261 agcagtcccg cttttccgtt catgcgccag tggtacaata ccgtggcctc gggtttgcgg
    37321 aaactgttgc gcaataaaaa acccacctca ggattaaaac cggtgcccag ctcgctgtaa
    37381 cccatgatga cattcagctt attccactca taattgtaaa gcaatttgaa ggaatgattg
    37441 gactctttga tgccgggggt ggcggttttg gcaaaaaaac ctgaaaactg ggctttattt
    37501 cccataccca gtttcccgtc tatggcaaac gtccggttgt aatggtcttc cccgggcccc
    37561 atgccctgtt tgttgataaa aaggcctccg atggtggacc ttttggcaaa ttcatgattg
    37621 acccgggcaa tgctgaaatt atttttggct attcctgctc catccacctc gtccgtgaac
    37681 agagaaagta aacccagatt ggtatgattg atcttgccgg acaagcgggc accgcctata
    37741 atgggtacct gcacccctcc ggagccgatg cctatccggc ggctgaaaaa caagtccacc
    37801 tcaccagtgc tcccgacccc aaactggccc gcattttcca ggaaaaaagg ccttttctcc
    37861 gggaaaaaca ggttgaaacg gtcaatattt acctgttgat cgtccacttc cacctgggca
    37921 aaatcggtat tgtaagtcag gtcaagggtc agggcaggtg tgatgctgta tttgatatct
    37981 cccccgaaat tggcatcggt ggtggttttt aaaggaacca ccaggttgtt ggaagtggtt
    38041 ttacccagga cataagggat gactttgagg ttgccggggt tttcaaggtc taggccggtg
    38101 agcgtacctt ccagggaaag ccggttgagg ttaaactcga cagggatggg cgaccagtag
    38161 gctgcttcat tgtttttctg aatattgcgc tggaagttga cgccccagac ctggtttttg
    38221 ccgctgttgt aacgaagagt ccggaagggt atggcaaatt cagccatcca gccgttttta
    38281 tccaccttgg ttttcacggt ccaggaagca tcccagttga cgttgatgcc cccgacccgg
    38341 ccgccctgct ggcgattggc gttgggattg ctttgaccct cgttgtccac ctgggcatcg
    38401 tattcaatcc cggccggatt ggtcccgaat acaaaaccgt tctgaccgtc tttatacgtg
    38461 tccaggataa ataaaaaagc gtcggtggca tccaggctgg catccctgcg ggaatcggaa
    38521 atgatgattt tatcaggggt tgcatcataa caagcggcag acaggtaaaa aaagtcccgg
    38581 tcgtacgcga tccggatttc ggtatgttcc gaaaccggtt ttccaagata tggctgggtc
    38641 tggataagtt ctacaaaagg cgggatggtc tgccagacgg aatcttccag gatttctccg
    38701 tccaaaacgg gcgccttgct gaggagatag gcttttgtag tgggccggct gctcgaataa
    38761 ggagtattca ggctgttttg tgaccctgcc ggtgaagctc ctgcaaccag gctgatcgtt
    38821 atgaaaaatg cctgcaaacg gataaacata tcatttgacc agtgagaaac cgaaaaaaat
    38881 ctccctgctt tgcaaatgta tcaaacccgg attattcgga atagaattaa tggatttgaa
    38941 tttccgccag gacgggaaaa tgatcggaat accggactcg ctggatttcg acccggttga
    39001 cctgaagggg ttttgaaatt aaaatattgt cgatatggag aaatggcaat tttccggcat
    39061 aagtataggc cagaccattg ccgccctgca ggaaaccgtc catgaggtcc tcatgcaaaa
    39121 gccggtaaac ataggaagcc gggatgtcgt taaaatcgcc tgccaggacc agcggatgag
    39181 ggctttgccg catatgtttt tttaattctg cagcctggtc agccctgagg agaagtgatc
    39241 ttttataacg ccgtaaaaca ttctttactt tttgccaggt ggaccggtcg gtcaggtccc
    39301 gggtttgcag gagctgctcg gtctggtcgg atattttatt ggacaccagg tggacattgt
    39361 acaggcggaa agtgtctttt tgatagacga tatccgccca ggtacatcca ttgcccaggg
    39421 ccttgctttt aagctcccct tcccggatga tgggccagcg ggaaaaaatg ggccagaaat
    39481 tggtacccgt cagctgcgtg tgtttgtatt tttttatcaa ggaatccctg aaccggaagg
    39541 ccggagattt cctgtgccat tcctggaaac agaggatgtc gggattcagc cggtgataaa
    39601 acgcccattc cggcgcccgg ttgcttttaa aactggatcc gatattatgg gtcaggagcc
    39661 gtatcccttc ttcccccggg ggaaaagaaa aattgaaaaa cgactttctg aaaaggccgg
    39721 cgctgagcag gagcaggagc accagcaacc aggctttatg ttgtttccat agaagcccta
    39781 atccggccag gatcaggcaa gtgagataaa gatagggaaa caccagcccc agaaaggcgg
    39841 caggccagaa ttgtcttgga tcgatgcggg gggaccacca gctggccagc aggaacaatc
    39901 ctgcaagaat gctgcccgca ttaaagatcc tttgaaacgg tttactcaac gctgcaccgg
    39961 gttatcaaat tatttgctgg ccttttcgag ggtatggcgt tcttctttgg taaggctgtc
    40021 catgccttgg gtccgtattt tatccaggat ccggtcgatt tcattccgga gctggtcttc
    40081 atcgtcctct tccggcatgg gtttcaccag cttgcttttg cggccagccg ccggagctga
    40141 aaccgcttta cgaattggga tgatttttgt ttttggtttg tttgtaaagg gccaacccgg
    40201 aagggcggaa ccgtttttta gccaccagat gaaaagaatg ccgcccaaac ctccgcccag
    40261 gtggccgata tgcccgccgg tattgctgtt ttcggccagc cctaccaggt caaggaggac
    40321 cacgaccagg gcaatgtatt taagtttgac cggcccgatc aggatcaggt gcatcaggta
    40381 atcgggtgca atgatggtgg cggccaggac aaagcccatt actgctgcgg aagcgccata
    40441 ggcgaccact tcggccgaag gataggcaaa gatttgggaa aaaatataaa tactgaaaac
    40501 acccatgcag ccggatacga ggtacaaggg aagaatgacc cggttgccga tcaggtcgcc
    40561 cacgatattg ccgaaccagt acagcatgag catattccaa atgaggtgcc agatcccgat
    40621 gtgcaaaaac atatgcgtga tcaggatcca gggatgagtc agtaaatacc cggtgttgga
    40681 atggaaagat aaagcttcgg taaagcttgc gaatcccgcc cgtccttctt ctccggcgat
    40741 attgaacccg atgcggacaa aaagcaccag gatgaatacc gatacattga ccagcatgag
    40801 ctggttgaga cgggtgcctt ttccaagatg gtatttaaaa tcctgccaga tggaatcaaa
    40861 catataccct gctatttacc ggtttttcgc caatagagga tgatcaggaa accaaataaa
    40921 gcgcctccca ggtgtgcaaa atgagcgatc tgggtctgag cgccctgggc gaccatcacc
    40981 agttcaagca caatggcaaa aatgaccatg tatacggcct tcatggggat gggcggaaat
    41041 aaaagcatca gttgctgatt tgggaaatac atgccaaagg cgaccaggac accaaaaagc
    41101 gctcccgatg cgcccactac ggagcctgaa ttttgcatat accgggcatg ttgaaggaca
    41161 tccagctgac tgacttcaaa ataccagaaa agcagatgga tcaaaatggc tccgatgccg
    41221 caaaagaggt aaaaaaataa aaatctgcgg ctgccccaga gcgcctccag catgggacca
    41281 aaggtggcca gcataaacat gttgaaaaac aggtggggca gattgccgtg cataaacata
    41341 tgcgtcacca actgataggg cctgaaatcg ctggaccccg ggtaataaag ggccagatct
    41401 ggaagttggg aataaaccgg aagtctgctg attaagaaaa ggataccgtt aatgatcagc
    41461 aattgtttga ctacatcagt caatcgaatc atggggtaaa attagttgaa attttgaaga
    41521 tggccggttg tagagatggc tgggcgtatg tcaaaaagca gatctcgttt aaccgaaaat
    41581 gtaaaacctg cattcagaca tgcacccggc ttggctcatc ccatcccaaa ctaccccagg
    41641 ttaggacagt gaatatggaa ataagtttgg gattccccgg aacctctcaa cccgccggtt
    41701 tcaaccggct cgttgccctt ttacctcaat actccacttt taaggtccac acatattctc
    41761 ccctggactg taattccgaa ggcaagacga tttccaggcc tgccggcgtc tgttcccatt
    41821 tcagatccga atgcacgccc agcatgctta ctccggaaat cgggcccggt ttcaatttgg
    41881 tgaggatgat tttgtctttg ggaaaatcaa aaagaaaaat ataactggct tttccatcct
    41941 tattttgagt caggcggacc ctgtccccgt caatataact cgggcccatc cgggtgttgt
    42001 atatgccctc cccgtttatt ttcatccagg tgccgatttt tttaaggcgg tcgtaagcgg
    42061 cagggtccca ttccccatcg ggtcccgggc caatgttcaa caataaattt ccacccttgc
    42121 tcacaatatc gaccagtttt tcgatcagtt catcggcggg cttgtacaga tcgtccggca
    42181 cataactcca gctgttggcc atagtcatac aggtttccca gggatagggc aggccctttt
    42241 cgggaatatg ttgttcaggg gtcaggtaat tttgatggac tccgggcacg gcacggtcga
    42301 ccacgatcag cccggcttgt ttttgccgga cttcccggac aatcccggcc atgtcgatgt
    42361 cctggctttg gggattgcgg gcataacggc tgccctcata aacttccaat aattgtgctt
    42421 ttatttcttc atcggtcttg ggtcgcaccc agcctccgtc cagccacagg atatcgagtt
    42481 ttccgtagtt ggaggccagt tcattcaact ggttgcgggt gaagtcggca aagtttttcc
    42541 agcgttccgg gtatttgttg atggaataat tcgggttgcg gtcggcggga gggaatttgc
    42601 tccaccagta atcgttgtta tgccagtccg gtttggaaaa ataagctcca gcccagaaac
    42661 cctctttgcg aaaagcatcg aatatttctt tggttacatc gctccggggg ttgcttgaga
    42721 aaggcaccag cttgccggtt attttataat cggaataccg ggtatcaaac atattgaaac
    42781 cgtcgtggtg cttggtggtg aacacgaggt atttcatgcc cgctgctttc gcggcggcgg
    42841 cccatttttc cggattaaat cgggtgggat taaacgtaag cggcagttct tcgtattttt
    42901 tacggtattc gtcataatct tccgtgatcc cttttttcca ggcccaggcc gcccatccca
    42961 catcttcagg acaaatgctc cagctctcta caacacccca ctgactgtag gggccccagt
    43021 gcatcagcag gccgaatttt aaatcctgcc actgttccag tttttgttga atgaggggat
    43081 cggggtccgg ataatattcc tggacttgcg ctttggaaag atgaaagaac catactaaaa
    43141 gaaggaggaa atgagatttc ataaaggatt gttggaataa ttaacctgag gctgcggcat
    43201 gtcctgaccg ggtgaaatta gttgaatttt tgagactttg aaggagaagg aaaacacagt
    43261 aaatagggat aactcagaag ttaaaatcaa tcttttacgg cattcccggt aactacttgt
    43321 tttttaaata cattatttta cagaacgcgg acaccccgtt tacccttgtt ttataaacct
    43381 ttaaaactcc atattatggc tgctaagctt gcattatgca ttggaatcaa tgattaccct
    43441 gaaacgggga gtgacctggc tggttgtgtc aacgacgcca acgattgggc ggacaccctg
    43501 actgcccgcg gatatgttgt aaaaaaactg ctggataaaa aagcgacccg ggcgaatata
    43561 cttggggaaa tgaaatccgt catttccgca gcaaaaaagg gggactccat actgatccag
    43621 tattccggcc acggcaccta tattccggac caggacgggg atgaaccgga cggtaccgaa
    43681 gaatgcctct gcccttatga tctgaaaacc cgcggggtga tcaccgacga tgaattgttt
    43741 gacttgttca acagcaaggc tgccggcgtc aggctggtca tgatctccga cagctgtcat
    43801 tccggaacgg tgacccgttt tgccccgccg gcggtcagag gccggaaagc gccgcagacc
    43861 agggttaaat ttctgccccc ggccagtttt ttatcctccc gggaactcaa caaattcgga
    43921 gtttccaggg ccatccgaaa atcctctcct cccggccgtt acggcggcct gttgatgtcg
    43981 ggatgccagg atgtggaata cagctatgat gcttatttcg gaggccggcc caatggcgct
    44041 ttcacctttg ccgctcttgc aacactgaaa aagctcaaat ccggagcctc ttataaggac
    44101 tggcacaaag ccatccgctc catgctgcca tcccaacaat acccgcaatc tcccaatctt
    44161 tttggttctt ccacgatgaa gaagtggaag gtgctgcaat aaaccggaat cttaatatgt
    44221 ccaaccgtta taccaggtta ccggctacgg agagcaggtt tttctccggc gcggcgcccc
    44281 ggctgaggcc tttcgattac taagaaaacc gggagcgggt gcagggttat ttttatactc
    44341 cgcccgccac ccggttttat tatgcggaag cccagcagaa aaaaaggatc gtcttgcatt
    44401 tcaccgcagg caatctggcc ggcgatatga atcaattgac gatccaggat taccacgtat
    44461 ccgttccttt tgtcattgca cgggatggga aaatttacca attgttttat tccaaatact
    44521 ggtcccatca tctcggcttg aaaagcggca acccggccaa ggtttatgac cggcagtcca
    44581 tcggcatcga aatctcgaat tacggctggc tagaatccag ggacgggcaa ctggaaaccg
    44641 cctattccag gcagggatca aacccatcgg acgtctattg cggcttgggg gataccgaag
    44701 cttatctgca attgaaccgc aggtaccgtg accatgaata ttatgcgtcc tttaccccgg
    44761 cccaattgga aagcaccatt gtcctggtcc gttatctcct tgccctgtat tccattccgc
    44821 cttccttcct gccgcccgcg gagcgttatg aattcatccc atccaatatg ggatttgcag
    44881 ggatactcag ccatgtgaat tatcgagata acggcaaatg ggacattggc ccggcctttg
    44941 actgggaggg cctgatccaa ggggttcagg cggaatcgtt cgtgcccgtc ctgaatccct
    45001 taaggtctgt acggggaaaa ccatcccaac aagaaaagga atttgcccct gcacaaaacc
    45061 ggggatcaaa atacaaaccg ggctccagga ccgtaatcag gcagtataat ccttttgact
    45121 gggaggaata aatgaatcaa accccatgtg ctaccgagat cgcggctttc aaccgatccc
    45181 agtccgtatc acccggcact gtacaatcag cacccaggat aaactgatca ggtacctgct
    45241 ttaatacatt ccggacttca ttttctatct ctgtccggct gcccttgccg atgaccccgt
    45301 gacgatccat accacccatg cagggtctgt tgaacatttc cgaaatctcc tgccaggtaa
    45361 gcaacctacc ggtaagctga ggattgcagt ttaccacttg cccgggataa tccaggacgg
    45421 catcgtaaga ggcataaggt gcattgtaat cacagacatg aaggatatta aaagggcaag
    45481 cagccgtgac ttctttcatg gccaccagat cggagggttt gacgtattcg gtaaataggc
    45541 ggggatcgct gaattgcccg gtttccgagc cctgggtgga catataaaaa ccgtccacgc
    45601 ccaggtctat gcaggcgcgt acaaagatca gctggctttc ggtcaggata tccaggccct
    45661 tttttacggc ttccggatct tcttccatgt gtttcagcaa cacttcgcgg gtggcggaat
    45721 ggccggccca cataaagggt gaatacaggg tcatgatgac cagcgcctct tttttggagg
    45781 cctgcacgat ttcttttacg gcctgcaact ggggctggta aaattcaacc ggattagggc
    45841 gcagttttga ccagtcggcc ggagttttca gaaaatcgag aaggggatat tgttgttcgt
    45901 attgaatttt tacgaaatcc attccggtgt ggcggaaata ccggaggtgt tcctgtgcgg
    45961 cggctgatcc cagtttaaaa tcatccccga aatgcagaaa aaaagcggcc ggagtataac
    46021 cgggctccgt tttcccctcc agccattgga gcatgcggtc cctattgctg agggtcgacg
    46081 tttgtttgca ggaggctagt gaactaagca ggggggatcc tgccagaagg gccccggtac
    46141 ccatgagtga agttctgagg aattgggatc ttttcataaa aaggagttaa tttaaagatt
    46201 cagttaaaat aataaaatct gagcatagag actgtttggg tgtatgacaa aaagcagttt
    46261 tacattttct ggataaacga gacctggtat gttttacttc gtttttatgc gagttgaaac
    46321 cttccaggtc ttgttatcga gtaaaaagac ctgcattttg acatgcaccc gactgtttat
    46381 ggctcattca ggacaacatt ggaattttta ctttcgattc aggtaaatgc tccatggcgc
    46441 cttattgtcc tttggaacaa tggtagcgca tttgatgttg aattaattga ttatcattaa
    46501 aataatgaac aatgaaaaaa ttaaaaaata tccaccctgg agaaatctta ttgaaggagt
    46561 ttcttatccc gcttgatata agcgcgtacc ggctttcaaa ggaaatatcc ataccgcaga
    46621 cccgcatatc tgaaattata aaaggaaaca ggcggatcac cgcggacacg gctttgagat
    46681 tttctaaatt tttcggaaat tctgccaagt tttggctcgg gcttcaggac gattttgaca
    46741 ttgaagagga aaaacaaaac aagcaaaagg aaattaattc aataaaacca ttggaaagta
    46801 aggtggccta atacggtttt accacaggcg ggtggtcgca cttcgagtta ataagatatg
    46861 taaatttgaa tattattgat caggaatgcg attgggtatt tgccattgcg aactccttat
    46921 gctggccggc tccaacatac tgacatcaat aaattgtgcc cgcaaaactc tttgaacact
    46981 tggaagttta actaaaatag gatgatataa ggaaatatac tcaataaact ttcgtaaata
    47041 acgatgttgg cactcatttt aaggacaact acatgaaaca gacattcata tttatattga
    47101 caatatttat tctgtcatcc tgcaatgagg aaaaaaaatc ggattcaaat ataactattg
    47161 acactgttag caacaccaca tctaacaaca cagttccgac aaatcaattg acaccaaatc
    47221 taacgtttga ctgttcaatt tttaagttta aaagtaagaa ggaacaagca gacacattaa
    47281 ctaaatactt tggaaaaaaa caattagcga ctgacgagaa atttttctgt gcatttccaa
    47341 actcatttaa cgagatgcaa aatatctttg gctttgacga cattagaggt gaagctccaa
    47401 tgtatgacta tcctatggga gaaaatatta ttcgatattt tgctaacctg aacactattg
    47461 acaaagaaat atattacaac aaatacataa atatttgtgt ggacggaatc tgggaggctg
    47521 acaacattag ggaagcattt ggattttcaa atagactagc aaacgacaca gaagctggtt
    47581 gtttatcatt atctaaaaga accgacaagg agattaaaag cgttttccac tttatttttg
    47641 acggacctca tccaaaaaac gaatacaatg aaaaaattta taatgagctc cttcctattg
    47701 tgaccaagca aaacgacaga cttggtaaac tgctaactga atcctataaa aaagtcataa
    47761 cagaggacgg tggaaacgga cattagaaaa acgagtgcaa acacgggttt tgcatcaggt
    47821 ggggttggtg ctaacgtgaa aatctcaatt catatttact tggtgcgggg ggctgagcgg
    47881 cttcgattcg aaactttttg caaggttaaa gttttgttct tcgattgaat ttttggttct
    47941 aaaaatctcc actttcggta atacccgaac tgttatgtga cattttaaag acggaaaaca
    48001 atgataaaac aattaacaat ttttgctttc attttgacaa tttgtttgat tagttgcgga
    48061 aagacaaata cggaaagcaa aaagacggaa aagctgacaa caattcagat tgacacttct
    48121 gttattacta tattgccatt agacacgaac ctacattgga tttttaaaac agggatgcca
    48181 actgaactaa cgacagaaga ccttttgaaa attgaaacca ttcttaaaaa atgtgttaac
    48241 gaatacaatc ctgagcaaga aagacagttt aaggaaataa acgacaaaca cccagaatac
    48301 aaacttgaaa ggaaaagttt cattattgac ttggcacgct acaaaagaca gtatatggcc
    48361 atagttaact ctaaaggtga aaaagagatt tgggtaaatt gtttttgtgg aaattgggac
    48421 aaaaattcga gaacaagccc agtcatggtt gatgacggtg ggaattgtta ctttaatcta
    48481 aaaatcaact tgacaactgg acagtattac gaattgatgg taaacggaga tgcttaacag
    48541 acaaagaata atgccacatg acaggtattg cgtcatacgg ggtgaccccg ctttggcggg
    48601 gcaggcgctg caatcccgat agctatcggg atggagcttt gtgcttctat tcaagttcag
    48661 tccaggttga tggttttgtg ctccaaaatc cgctgctatt taaagccgca aaacattaac
    48721 ttcgtactta agaataagca ggaaaaaaaa tccaattgga cgcctccaaa aatccccgcc
    48781 tatttttact cgaaggcctt caacgattca taaaacaagt cacaaaactg gatggtgtaa
    48841 tccgcatctc gcttctggga tccatcgtga ccccgaagac taatcttaaa gacattgatg
    48901 tgcttgtgct tgttgccgac cacatcgatc tcgctcccct ggcgacttat accaggcaat
    48961 tgatgggaca ggtgcaaagt ataaaccggg gagctgatgt atttctggcc gatgaaaggg
    49021 ggaattatat cggacgaatt tgtcattgga gagattgccg tccaggtatc cggcgggcct
    49081 gccaagccat gcattgcggt cgacggacct atctttacga tgatctttca gtggtcacct
    49141 taaatgctga aatggtacgt aatccaccgg tgaccctgtg gccaacagtt gagatacgat
    49201 gtccattacc aagggatgtg agggaatgga ttgaaaaatt atgatagctg tcaaaggatc
    49261 atgtcatgaa accgcaaaga gtgcggagat cgaagagtgg gcgaaaagaa aaagattata
    49321 gcatttggac tcactttacg tgttttgcgt cctttgcggt taatcaaagc gattaatgaa
    49381 accgaagaaa gcgctgaggt ggcagagtgg gcacaaagaa aaagattatg gcctttgggc
    49441 tcactttgcg tgttttgcat ccttttcggt taatcaaggt agtcaatgta aacgcatttc
    49501 caattcttat caaaagaacc ccaattaatg tcgtaatttg gaattcagta taagcgatga
    49561 aatataatcc aactccgttc agcgcacctc atcgtcaatt agaaccccat aattgaaata
    49621 aatcagacaa ccagatcttt tgtattagct ttatctaatc ggaaatcatg aataaatcct
    49681 ccacctccct gacggtcttt ggcgtttatc ttttgggtat gggcttcgga ctcgttttta
    49741 tgcccaatac cctgatcgga atattgggtc tgccacctac cgatgaagtc tggatccgcg
    49801 tgctgggggt cgtgaccctc gtcctggctt attattatat ccaggcggcc aggacaaacc
    49861 ttcgcacttt cgcttcatgg actgttccgg caaggatcct tgtatttttc ttttttacag
    49921 gatttgtcct cgcgggctgg gtgggtccgg tcatgagtgc gctgggggca atagatctgg
    49981 ttggggcatt atggaccgct tgggccttga ggaaggaaaa atcttaaagg aagtgattca
    50041 cggaagcatc ggagtcagaa tgacaatgca atggatcaat acagggtgta atttaggctc
    50101 taacctgagc tacaaactcg ccatatcaat cacaaaccgg taccgcacat ccccctttac
    50161 catcctgtcg taggctttgt ctattttctg gatgggtatc atttccacat ccgagacaat
    50221 atttttttcg gcgcaaaagt caagcatctc ctgggtttct tccgtgccgc cgatcatgga
    50281 accgatgatg ctcctgcggt ttttgatcag ggcaaaagga tccaggacgg gaaattcagg
    50341 aggcaggcct accacgagca tttttccatt cacgcccagc aggtccaggt attttttata
    50401 atcatgctgg gcggaaatgc aattcaaaat gacgtcaaac tgcccgttgt gctttttcat
    50461 ctgcgcctgg tcactggaga gcaggaaatg atgggcgcca agccttttgc cgtctgtttc
    50521 tttggaaggc gtatggctga tcagggtgac ttccgctccg aaagccgccg caaatttcac
    50581 tcccatatga cccagtccgc ccaatccggc cactcccacc cgggtatctt tgcccactcc
    50641 tgcatacttt aagggcgaat aggtggtgat gccggcacac aacaggggcg ccacggcgct
    50701 ttgatccagc cggctggaaa tatggtatac aaaccgttca tcggtgacga tctgcgaaga
    50761 atacccgccg cgggtatatc ctgaaccgtc ccgggccatt acattataag tgccggtcgg
    50821 gccttcatcg caatagttct cctggtgctg ccggcagctg ggacaggagc ggcacgaatc
    50881 gacgatgacc cctatggccg ccaggttccc gggcttgaat ttagtcaccg caggaccaac
    50941 ggatttgacg gtgcccacga tttcatggcc gggcaccaat gggaaatggg taccatccca
    51001 ttcccccctg gcccggtgaa ggtcactgtg acaaactccg caatatctaa tttccaaatg
    51061 cacatcgtgg ggccccactt ccctgcggtt gaattcaaat gggcgaaggg gttgatcggc
    51121 ctggtaggcg gcataagctt tggtggctat catggcggaa aattaaactt ctttgagaat
    51181 ataattgagg ttgaggttaa tgttgaggtt aaggttaagg ttaagggaat ggcttctcct
    51241 caaccctcaa ccctcaatct tcaatcctca ttccggcagg accacctcct ctccccagat
    51301 atatttatta aaccactgcc aattgtgcca gatcgcggca agcctttcct tgggtttggt
    51361 gatcccgtga ccgaatcctt tatatacgat cagtttggag ggcacctttt tgtcctggag
    51421 tccccggtac aattcatagg cattgggaat gggcaccctt cggtcgaatt ctccatgctg
    51481 gatcagagtg ggggtggaag ccttgttgat attattcatg ggagaggtcc tcagatagat
    51541 tgccggatcg gaccaggggg tggattggag atactgcctg gtgaaggggg taatgtccgt
    51601 atttacataa taggtggacc agttggagat tccggcgccc acggagatgg ctttaaaaac
    51661 attggtgtgg gtggtgagga aggcggagat gtacccgccc tggctccagc ccatgcaacc
    51721 cattttggtg gtgtccacga tccccagttt gtttacataa tctatgccgc tcatgacatc
    51781 ccacatatcc ccgatgccga ggttgcgtac attcagagac cggaacaact gtccataacc
    51841 tgccgatccc cggtaattga cccggagcac caaagcccct ttttcacacc attgcatcac
    51901 agggtataca taagagggcg tgggttctgg ccggtcgacc ccggtaggcc ctccgtgaat
    51961 caggcaaagc aaggggtatt ttttatccgt acggaaatca cggggccgga tcaggacccc
    52021 ttcaatctct gtaccgtccc tgctttgcca gcggatgatt tcactggtcg gggtcttcca
    52081 gttcttgatc tgatcggtaa aaaaggtaag ttgacggctg gaagtattaa aattatacaa
    52141 ggcgatttca ttgagtccgt catacatgcg gccggataag gcacaggtct gcccatcctt
    52201 gctgaaggta gcggtattga atacatcgta aggcacttcc gatttgctcc agagatcgat
    52261 ttcaggatcc aggcggaaca gttgcgaagc ggttttttga gaagctccaa accagatgcc
    52321 tccccgggtc cagtcgagat aggacttgtt ttcatcaaag gccttgccta tttctttggc
    52381 agaagcagag tccaggtgcc ggataaaaaa cctgttgttt ttgtaataaa aagaaacggt
    52441 atcgtccacc gcactggaat aaatcaactg cctgctgtcc ggcgaccacc cgagaaaaaa
    52501 atcaccggaa ggattgtcca ccagtatttt tgacgacctg gtcccggtct catacaaggc
    52561 aatatcgcca aacaccccgc tgttgatcag cgggtccggc aaacgggtat aagcaatgta
    52621 ttttccgtcc ggggaataac catatcctgc gacagaataa ttcgaatcgg tcactgccgt
    52681 cgccttgggg aaattaaaac agggaagttt agttttggaa acgctgtcgg aataacaggg
    52741 atacaaacca taccggtcaa tcgaatcctt gtcaaaggga atggtccaca ggcggttcat
    52801 ccggaattcc tccccttctg tgccaaaggc gccgaaccgt tcttttattt gtttgtcccg
    52861 cttggtttca ctttcggttt tggaaaacaa tatttttttt ccatccgggc tccaggtgaa
    52921 actgttgatc ccctctgttt cattggagat ggccaccgcc tctccccctt ccagggaaag
    52981 caggtggatc tgacttttgt tgccccggtc ggtgagaaaa gcaatccatt ttgaatcggg
    53041 gctccaacgg gcgccaaagc tgcttccttt tggggtgcgg gtcagttgaa agggagcctg
    53101 gccatttttg gaaagccaga tttccgtgtc gtaaacgttt tccttccagt cggtggaagt
    53161 tacgctaaat aaaacatgtt taccgtcggg ggagatctgg ggagtcccgc tttgtttaag
    53221 gcttatccat tcttcaaagg agaccgaggc atcctggccg gtgagcatac cgggtaataa
    53281 ggccaggaaa aaataataca ggtacttcat atttgggaat taagaggtca atcaggatct
    53341 ggctttctgt tttttttgtg atttggtttc aataagggcg gtccatatgc tcaacttttt
    53401 atccatatag gaagtccata ccggccgcac ccttccatta gctgcggaaa tattgttgta
    53461 atcaccgaag aacgcttcat ttccgggagg agtgaaggta ttgccttcca gccggagttc
    53521 tttaaagctt tttccaccgt ttttagaata agccactacg acatcggttt gcaagttgcg
    53581 gtgccggctt ctgtcataat acacgatata caggtacccg gtggattggt ccacacttaa
    53641 ccaggggaag aactgaatac tggaagtcct gtcctggttg acccggacgc gttctgacca
    53701 ggaattgccc ccgtcacggc tggaaaggag ccagatatcc atattgtttt ggccgttttt
    53761 gaggtcggcc cagcagatat aaatgcttcc ccggtatttg cccggactca gatccgtggc
    53821 caagaccggc attccgtttg ccctgccgac ccccggaact tccagggacc agcctcccgg
    53881 ttggtccacc agggcctggt cggcaagcaa ccaggtgttg ccgccatcgg cgcttttatc
    53941 aaaccacaat ttttgatcat aggcccagca cacatagatt tcgttggacg gtccgaatcc
    54001 gggcaccgca ccttccgggg tgaggtcgtc atcgaggcaa ttgccttcat ggtcgctgag
    54061 ggtcacggct tgcgtccagg ttttcccctg gtctttggaa agggaaaaac ggatccggga
    54121 gcggtcggtt tctttttctg aaccgtattg gtcaaattcg gtccaggtta cgaccaggtc
    54181 gccattattg ggattgctgc tgatccaggg tttgtcctga tttttgggag gattggttcc
    54241 gatggcggat ccctggtccc aggttttccc tccgtccgag ctttgatgga tgaccaggct
    54301 ttccaaccat ctccctgact gcctgcctct tccgtcggga ttgccgaggt gcacataata
    54361 aaatcttcct tcgtgatcgg cccagactac gggatcacca tacacaccaa gcggtgaaag
    54421 gagccggcta tgagaaaaac tctttccgcc gtctgttgaa taatgtacgc ggtcaagaat
    54481 ggaaccggcc accacgatat cgggattcac cggggacacg gcgatgctgg gttcagaagg
    54541 cccaaacctt tcccccttgt tctccgctat ttttaccgat cccagataag ccggggtcga
    54601 acaggaaaaa caagcggccg cgagaaaacc ccacaaaaag tatttaatcc ccgaaaggaa
    54661 cataaaacca aaattacttt attacgaaga aaaccggtct gaaacagaca aatcatgaag
    54721 cggttactta taagaatgtc attaatatat atacggcggg cgtttcaata gaagggggat
    54781 gagaagctga cactatttta tctatccttt tcatttatta ttcctttgac ccataattat
    54841 agttatggat gtaccgaatt caggccaatc gctttacatt ggtctgaaga agaggaatta
    54901 agacacataa aaatggatca taggtcgccg ctccggatca tacactgcct gtggctcatc
    54961 tattgtttga atttgcctct cagcggtttt tcacaggtgg ataaaaaggc cacggctgaa
    55021 accaaagccc tgtacggaaa tttaatgaaa ataaggtccc ggggtgtgat gtttggtcac
    55081 caggacgcac tggcctatgg gctgaatgcg gacctgagcc gctggattgg ccagcccgtc
    55141 aaaacagata tccgctccga tatccggacg cttaccggcc agcatcccgc cgtggtgggc
    55201 catgacctgg gacatctgga actcggcaaa ccggaaaacc tggatgccgt cccattcgat
    55261 caaatgcggg aatccataat tgccgtttac cggtatgggg gaatcaatac catcagctgg
    55321 catcccaaca atcccctgga tttgaccaag accacctggg accgggtgga ctccaccatc
    55381 cgcaggatca cggcggatag gaaaaacctt cgcaactata aaaaaatcct ggacaaactg
    55441 gccgtgtttt ttaagagcct gaagggcccc gggggagcat tgatccccgt cattttccgt
    55501 ccttaccacg aacacaccgg cagctggttt tggtgggggg cgggacattg taccccttca
    55561 gagtacaagg ctttctggca agtgacggtc aaacacctga cccggaaaag gaaggttcat
    55621 aacctactga ttgcgtattc caccgacaat tttatcaatg aagcccatta ccttgaaaga
    55681 tatcccgggg atgaatatgt ggacctgtta ggtttcgaca cctaccaccg gaatgccccc
    55741 caatccgact ccgcgtttac cgccaatgcc aaacgtatgg tggctaccct gaaaaaactg
    55801 ggcgaagaaa aaaacaaatt atatgccatc accgaaaccg gcctggaaac ggtcaaagaa
    55861 cacgattggt ggacccggat cgtttatccc atcatcagcg ataccggact gagttatgtt
    55921 ttggtatgga gaaatgggag ggccgatcat tattatgcgc cctttgaagg gcagcaaagc
    55981 gcggaggatt ttaaatcctt ttttaagctg ccgcaaacct tgtttgaaaa agacttactg
    56041 aatctttata aaccctgaag taccggaatc atccggactc gatcatttta aaaattatga
    56101 attttaaaga gcggctgaat gcattgaaag ccgaaaagga agttttgctg aaccggagca
    56161 atgagatcgt gcttcctggc aacggcatct atttcagata caaaaatccg gtggttaccg
    56221 ccggtcatgt gcctctgttt tgggagtatg acctcaatcc ggaaaacaat cccttcctga
    56281 tgaaacgctt tcccatcaat gccaccttta atgcgggagc catcaaatgg aagggcaaat
    56341 atcttctcgt cctccgcgtc gaaggggcag accggaaatc cttttttgca atcgccgaaa
    56401 gcccggacgg gatcagccgg tttacgtttt gggaccatcc ggtcaccatg ccggaaaccg
    56461 ggaatccgga tatcaatgtg tacgacatgc gactcactgc gcatgaagac gggtggatct
    56521 acgggatttt ttgttcggaa cggaaggacc ctttcgctcc gaaaggcgat gaatcgtctg
    56581 cggtcgcctc ggccggcatt gcccgcagcc gggacctggt aaactgggaa cggttgccgg
    56641 atctggtgac accctctccc caacaaagaa atgtagtcct gcatccggaa tttgtccagg
    56701 ggaaatacct gatttatacc cgtccccagg atggttttat tgaggcgggg agcgggggag
    56761 ggatcgggta cgggtattcc gattccatga atccggccat tattgaaaaa gaactgatcc
    56821 tggatgaaaa aaggtaccac acggtatatg aaataaaaaa cggcctgggg ccggcaccga
    56881 ttaaaacctc caaaggctgg ctgcacctgg cccacggggt cagaaatacg gcggccggct
    56941 tgcgctacgt gctttacctg ttcctgaccg acctggccga cccgaccaag atcctttata
    57001 aaccggcggg ctatttcctg gcccccgaag gagatgaacg ggtgggggat gtcagcaacg
    57061 tggtattttc aaacggatgg attgccgatg aaaacggcaa cgtatttatt tattatgctt
    57121 cttcggatac ccggcttcat gtagcaagtt ccaccgtgga acaattgatc gattacgcca
    57181 tgcatacctg cgcggacggg ttccggtcca tgacctcagt aaaaaccctg aataacctta
    57241 tagaccggaa catcaccctg caaggttgac caccggttgc ctatggattc aaaacttcaa
    57301 agttttaagc tggagatccg ggaggaaatg caacgaatcc tggagtggtg gaggaagtat
    57361 tctccggatg agcggaatgg aggttttgcc ggagaaatag atcagtttaa ccggcagttg
    57421 ccccaagccg ataaaggcag cgtactgcat gcccggatcc tctggacttt ttcctcggcc
    57481 tatcgccatt cccggcagga ggttgacctt gaccttgcct ttagggctta tgaatttatt
    57541 gtccaaaaat tttatgacaa aacctggtct ggtgtgtttt ggtcggtccg ggccgatgga
    57601 acgatcctca gcgaccggaa acaaagctat gccatcgctt ttgccattta tggcctggct
    57661 gaattttata aagtgaccgg tgatgccgga ggccttcaac tggccgtgga attgtaccat
    57721 gcccttgaaa gccggagcct tgatcctctg gagggcggat attttgaggc ctttacccgg
    57781 gattggcaaa aactggagga agtgcgactg agccaaaagg accgcaacga tcccaaaacc
    57841 atgaataccc acctccacct catcgaagct tatgccaatc tttaccgggt atggcgggat
    57901 gaccggctgg aacattccat ccgccatctg cttaaggttt ttgaacaacg gatcatggat
    57961 aaaacaaatc atcacctgcg gctatttttt gaagcggact ggaagccgac ttccgaaagg
    58021 atttcctatg gacatgatat cgaagcttcc tggttgcttc acgacgcagc gaaagagctg
    58081 caggacccta ttttagataa aaggtggtcg gccatagcca ttcaaattgc cgatgcttcg
    58141 gtcgggggat tacaggccga tagcagtttt atccacgaag cggatccgcg gtccgggtac
    58201 ctggaccagc accgggaatg gtgggtcagc gctgaaggca tggttggata tttgaatgcc
    58261 tggaaattga gcggcgaccg tgtttacctg gaacgggtgt acggactttg gaattttatc
    58321 aaaaaacacc tccttgacct ggaccgcggg gaatggcact ggggagttta tccggattat
    58381 acccggatgc cggaatataa aatgggcttc tggaaatgcc cttatcataa tttccgggcc
    58441 tgcctggaaa tcctggatct attaaaagaa taatatccct tcctcccttc ctcctttcct
    58501 ccctttctcc ctttctcctt cctccccatt atttatcttt gcgcgctcat tgccccgata
    58561 accgttagct taaggtaatc tgtcaaactc tatgcgaata ttaaaatttg gtggttccag
    58621 tgtcggcagc ccggaaagga tcctttcagt catcgggatc ctccgggact acacccgtaa
    58681 aaagatcccc tataccgtcg tcttttctgc tttcagcggg gtcaccgatt cattgatcag
    58741 gatggccctg gcggcggcaa aaggagatcc cgcctacgag gctatgtgga ctgttttcca
    58801 cgagcggcat atcctggcga ttgaatccct ggtcgatgag gaccggcgaa aaacccgcct
    58861 gagccagcaa ttcaatgaaa tggcaggcac cctgaagaat ctgttgcaag gtacgtatct
    58921 ggtaatggaa gcctctccgc gcaccatgga ttatgtattg agctttgggg agcgggcttc
    58981 ctgcatgatc atcaccgaag ccatgaacca gcggggcctg ccggcagaat gtatggatgc
    59041 ccgggaattt atcaagacgg ataaaaactt tggcgctgcc cgggtggacc acaacctgac
    59101 caacagcctg atccgtaaaa aattcaaacc ttccaaaaaa ataagagtgg tcacgggttt
    59161 tatcagcagc gcaaaaggcg gcttgacgac taccctcggg cgcggaggct ctgattacac
    59221 cgcttcctta ttgggagccg gcttaggcgc ggagaggatt gaaatatgga ccgatgtgga
    59281 cggggtcctg acggcggatc ccaggttggt ggacaaggcc tttaccattc ccaaactgtc
    59341 ctatgtggaa gccatggaaa tgtcccattt cggggcaaag gtcatctatc ctcccaccat
    59401 taaaccggcc atggacctgg gcataccgat tttcattaaa aacaccttca accccggatt
    59461 tcccggttcc ctgatcacca aagagaaagt aaaacaccag catccggtca cggggatctc
    59521 ctcgatcagc aatgtatccc tgctcaccct gcagggcagt ggcctttttg gagtgcccgg
    59581 cgtggcagcc cgggtatttg aggccctggc cgaaaaaggg atcaatgtca tgctgatcac
    59641 ccagggttct tccgaatcct ccatcagttt tgcagtcagg ccggaagacg gacaggaagc
    59701 caagaccgcg ctcgaccatg cttttgcacg ggacatcagc gccagcaccg tggaacccgc
    59761 ccacctggaa aaagacttgt cggtggtggc ccttatcggg gaaaatatgc ggtatacccc
    59821 gggcattgcc ggccggatgt tcaggtctct tgggaaaaat gggatcaacg tagtcgccat
    59881 cgcccagggc tcctctgaat tgaacatatc cgcagtgatc agcagggccg atgaagccaa
    59941 agcgctcaat gcccttcatg aaggcctgtt tttgtccgat accaaagtcc ttcacctctt
    60001 catcatcggg accggcctga tcgggggcac cctgatccgc cagatccagg cacaggccga
    60061 atacttgctg aagtccaaac acctggaaat taaaatcgtc ggcctggcca acagccgcaa
    60121 aatgatcttc gatgaaaagg gcctgaatac ggaaaattgg aaagaaaacc tgatgggaaa
    60181 aggcagtccc atgaaactcg cccgcttcat tgatcagatg aaacaaatga acctctccaa
    60241 ttccatattt atagacaata cggcttcaga gatcgtggct tcccattatg ataaaatcct
    60301 ggattccagc atcagcatca gcacacccaa taaagtggcg gcttccagca aatttgcccg
    60361 ttacaataaa ctgaaggaaa tcgcggacaa ccggggggtc cagtttcaat atgaaactaa
    60421 tgtaggggcc ggcctccccg tcctttccac gctgaaaaac ctgatgatct ccggcgaccg
    60481 catcctcagg atcgaagggg tgctgtcggg ctccctttcg tatatcttca acaaattcaa
    60541 aaccggaacg catttttccg acctggtcag ggaagccagg gaaaaaggct ataccgaacc
    60601 ggatcccagg gacgatttat ccctcaagga catcagccgg aagatttgca tcctggcccg
    60661 ggaagccggc taccgggtgg aactggaaac cgtcgaattg gaaaaaatat tatcggacaa
    60721 ggccctcaag gcaaaaaccg tggccgattt tttgaaaata ctggaacaaa acgattccca
    60781 ttacaataaa ctgatccagg atgccgaatc ccggggagcc aaaattcgcg tgattgccaa
    60841 ggttgaagcc atagcttcag gcctttccag ttgccgggtc tcggtgggcc tccaggaggt
    60901 atcccccgac
//
LOCUS       DAOQXT010000016        59497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_548_length_59497_cov_6.041334, whole genome shotgun sequence.
ACCESSION   DAOQXT010000016 DAOQXT010000000
VERSION     DAOQXT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 59497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59497
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_548_length_59497_cov_6.041334"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2044)
                     /locus_tag="PKM27_04315"
     CDS             complement(<1..2044)
                     /locus_tag="PKM27_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06516.1"
                     /translation="MIFSSLKFFEVYHLIRFAKIQRRRFKATPPGLFVILFNLVLLSG
                     LYAQTGFRGTVMEQETGNGLAGATVRIGERGIFTDFEGHFQLALEPGNYLVTISYIGF
                     KTRSVDITIPPSGWAEMDIRLEALIGILETAVISDSRYQKPLGESTVSLELIKKGLID
                     HLNSTSVDEVLDKVPGLNMIGDQANIRGGSGFSYGAGSRVLLMVNDIPALQADAGYPN
                     WDDLPVENIEQIEVIKGAASAIYGSAALNGLVHVRTAFARTEPLTRASLFYNPVMAPK
                     DKSQQWWSSAPYTAGMSLSHARKAGKWDLVGGLYALNNKSYNQTSRSDYQRVNGSVQY
                     RITDRLVAGTHFNINNREGNSFFYWKDGVHAFYRPDSLTLSQSNSFRYHVDPYVHYFD
                     QRGNKHSFLSRVYVVDNSVSSGQSNSSSLWYGEYRFQKAWTGLKLVTTAGLVYAHNRS
                     ESPLLNLLPLKSNQAAAYIQAEKKLGSGTTVSLGWRWEDYRLSRPEVLAGDTLLGGKK
                     RDHKNLFRLGLNTKVGEGTFLRASLGQGFRFPTLTEQFITTSFGSTFISPNPGLKSET
                     GWTAEIGAKQGFRINRLTGFVDGALFWSRYRDMMEFVFTGFVKGFQSQNIGNTDIKGY
                     ELSLGAGSTGRHRWSVLGGYTYINPKFVHFTEEDNRRSSADFNILKYRSRHLFKLDV"
     gene            2032..2856
                     /locus_tag="PKM27_04320"
     CDS             2032..2856
                     /locus_tag="PKM27_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06517.1"
                     /translation="MKKSWVLFILASFQMYNIRAQNPACLPDPVYKDSTAGIYPRPYN
                     DSTMTGGITRPVCIGQDYEYPLTVNIPDLITVPFGNTTITIGLESAILDTSNAVSGLP
                     KGIRYHCNPGNCIMAKNQLGCVVLKGRATEENTPGTYDLVINLKLITQAGSFDVAFPG
                     PFFPGKYFLTVTEPGSQACLTSPARELQADQVSHRAFPVPADDQLNIRVHAFQPGQGH
                     LILRDLSGRTLSDMPMSLDKGENSFSIPVSHLQNGTYWYTLQIGQSVNTRRFSVLH"
     gene            complement(2860..3354)
                     /locus_tag="PKM27_04325"
     CDS             complement(2860..3354)
                     /locus_tag="PKM27_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06518.1"
                     /translation="MSNALKSLNENEVQSLTNALPLIAILIGKADNTLDVKELDLAKK
                     ITHIRTYNSPEYLQAFYEKVEEQFEPLLAKFNSELPEDAALRGETISGKLAQLNPILA
                     KLHPKAGAFLYRGFVRFAQEIAKASGGFLGFMAVNPEESKWVKLPMINPVEYEDQENG
                     DEEE"
     gene            3498..6137
                     /gene="mutS"
                     /locus_tag="PKM27_04330"
     CDS             3498..6137
                     /gene="mutS"
                     /locus_tag="PKM27_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNR06519.1"
                     /translation="MARPSKNTKSVAREEGTTTPLLSQYAQVKSKYPDAILLFRVGDF
                     YETFGEDAIVAARVLGIIQTKRNNGATVTELAGFPYHALNLYLPKLIKAGYRVAICEQ
                     MEKPVPGRVVRRAVTEMITPGVITGDQFLDQKKNNFLGCLYFGNKSRHGLAFIDISTG
                     EFLVAEGDTSYVEKLLQGFNPAEIILARNSARLFESTWGNAYYTYPVDDWIFQHAYAR
                     EKLLEQFEVHSLKGFGIEDMDLAQVAAGAALHYLKTTENQIRHIRNIQRIPESEYVWL
                     DRFTIRNLELLESPLDKGKSFLQVMDQTLSPMGSRLMYKWVMFPLLDLRSINQRLDAV
                     TYFHRQYDQLDRYGSLIRQVGDLERLINRAAMDRISPRETVQLRRALEALPPFKDLLV
                     SSGNEELRHHGEALQLCAELRTNIEEKIKEDAPALTNKGGIFKEGCDAALDEHRYVVQ
                     HAQELLLQLQQQEYEKTGIPLKVGFNSVFGYYLEVTNRYKDQVPPGWVRKQTLANGER
                     FVTDELKKLENQILGAEEKIIDLEESLFRDLVNLIKEYVHPIQHDAQLIARVDCLMSF
                     ARLAYQNQYHRPVLDDSYIIDIRQGRHPVIEKNLPPGETYVPNDLYLDNENQQILMIT
                     GPNMSGKSAILRQTALICLMAQMGSFVPAESAIIGIIDKIFTRVGASDNISSGESTFM
                     VEMNETALILNNTSARSLILLDEIGRGTSTYDGISIAWAIAEFLHDSPARPKTLFATH
                     YHELNELADKHPRIKNFHVSTKEVGNKVIFLRLLKEGGVRHSFGIHVARMAGMPQSVV
                     SRAHNILKHLEQKSIEAAGPDTKTADKLKGMPAPFQLNIFESSDPRAQAVIRMLEETD
                     IDRLSPIESLMKLNEIKGMLKEN"
     gene            6187..8286
                     /locus_tag="PKM27_04335"
     CDS             6187..8286
                     /locus_tag="PKM27_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06520.1"
                     /translation="MITFTSRGLVPLFILSAWPAPSFLLYAQQDSASWKPTTLPFEEA
                     LQSLAEHGENPDDTDLGLATELLESWRKKPLNINDPDHPDLQDFLLLDPVRLEALRHY
                     IRSQGPLISLYELQVVPGFDTELIRRISPFIQLRPDLDRFTISLRDQFLSSQHQWLIR
                     LSRNLEPLTGSGTAAGTGFTGSPLALLVRFRHFYENRMSIGFLGEKDPGETFFRKDNK
                     AGFDHYSFHAGIRQVGRILEELVVGDYTVSLGQGLLMHAGMGGRKGALVTGIRKPDRV
                     FKPYTAAGEFNYFRGLCALLKPKPPFQLAVFYSNRQKDANTGSASMQGLGVITGFLES
                     GYHRSPAELEDEKRVNEQMAGGSVRHRWRSGHLAINLVSSRYDKFFKKKESAYTAFSF
                     SGHKLTQASLDWGIKFRHFNWFSETAFSSPAGIAIVSGLQTSLDKRLDLALLYRRYDK
                     KYHSFNANAFGEFADPVNEEGFYAGLQFAPASRWKINAYWDFWVRPWYSYQADGPSAG
                     NEQLVRVQYAVRYKMDSYLQLRFRSGEENGMEPSVLTPLLERRQAQIRIHLNLFHGKG
                     LETRSRIEFNRNKLAGAKASTGFLAFQDLLYQPVQSVFSGTARFCFFSTRDYDSRIYA
                     YENDLLYNYSVPAFSGKGLRLYLNQRWALSKQLVLEGRWACTFKFKSRLKDPDSYENE
                     GNKNMTLKFQCRWQFGI"
     gene            8426..10273
                     /gene="yidC"
                     /locus_tag="PKM27_04340"
     CDS             8426..10273
                     /gene="yidC"
                     /locus_tag="PKM27_04340"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNR06521.1"
                     /translation="MDRNNIVGFILIFVVLLLWSYFSTRQHQQEAIQTRIQDSITRVQ
                     IIQDSLRNQIRASGTGTDTLAMAAPAGPQAGMAGVLVDSTAKASDYTLENEVLKLTLN
                     SLGGQITKAELKEYKKMVVVSKGPDQKAPLYLLHHPSNLLNLTLPLRDRPLETKNLIF
                     SVPGSDPSSVTFRAVTPDGKQLDLEYRLHPGSYLVDFKISLNGFDQDLAEKEIVLDWQ
                     NYIEPLERGIAYEKTLTSLHYKPLADDKERLKAGGAEKESFEGPVSWISNAQQFFNTS
                     LLADEPFRNLTVETTPGPEGSEYLARFNSRVSIPFQVSGAQGLKWYIGPNEFGLLKAQ
                     GNGMEDIISFGASILGSINKWVIRPVFNFLSGFIGSKGIVILVLTFIVKLMLFPLSYK
                     MLHSQAKMGALKPELNQLREKFKGDQQAMQMESMKLYREFGVNPLGGCLPMLLQMPIW
                     LALYQFFPASIEFRQASFLWADDLSSYDVFAKLPVSIPFFGAHISLFTLIWVVSTLIF
                     TYYSTKDQDFSMNPAMKYMQYIMPVFFLFFFNNSASGLTAYMAFSNVLNIGQTLGGKA
                     LLFPETKMKEELHAARHKPKKKGGFQERLETMMKEQQKKMDERQKQVKK"
     gene            10270..10884
                     /locus_tag="PKM27_04345"
     CDS             10270..10884
                     /locus_tag="PKM27_04345"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HNR06522.1"
                     /translation="MKYAPPVFFLALLLLLACASTARLKPASPGSASPPPQAAIPENK
                     PAENEENKPPAAVMIPESTKSRDTIPPPAFKIDTLFTVSYGPCQGKCPVYRLSVTNDG
                     MVFWNGFQHVERTGPHITRLNEEQRGKLEAAIGRVFHSSLQASYPGKDEFIPTFPMKT
                     YTLKLNNQMKTIEVNHSPPRQLIDFERQLFSWLEKADWRPVPAR"
     gene            10928..11968
                     /locus_tag="PKM27_04350"
     CDS             10928..11968
                     /locus_tag="PKM27_04350"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNR06523.1"
                     /translation="MKEVVVIGAGSFGIAVSKLLAYNVRVRVFTRNPETLHKINVEHQ
                     MFNVALSERIVATQSMEEAIRSARVIFPIVPSDSFNAMIQEAAPFLSPAHVLIHGTKG
                     FVVKEFGEEDLGKSELRKEKVLTISELIRQQTSVVRIGCLSGPNLAKEILDGQPTATV
                     IASEFDEVVRIGTELLSSNKFFVFGSNDLKGAQIAGALKNIIAIGSGILDGLGLGKNI
                     QAILITRGLGEIIHFGQRMGAGSSAFLGTAGIGDLIATATSTDSRNYQFGKRLGKGEK
                     ITTVLNTSEELAEGARTLKIVYHLLKSFRMRSPIIEILYRTVYEDYNIQEGINFLMRY
                     PVKKDVDFIEED"
     gene            11987..12496
                     /locus_tag="PKM27_04355"
     CDS             11987..12496
                     /locus_tag="PKM27_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06524.1"
                     /translation="MKVIFRSLVFLILAGVLAFPFVRIMYIHLRVVSHRKIMQGEEMG
                     DHPVETLNLTVAELNRFRLDERELIYEGKRYDIRTEKRRGDSVRLFAVHDWYEQYLLQ
                     EIIDLHEKGKQPDQQVARMLTPDWIIVKILRLNGPNKPAHPLPFPEYLIRWMHTPWLT
                     WFPPPDGNI"
     gene            13010..15193
                     /locus_tag="PKM27_04360"
     CDS             13010..15193
                     /locus_tag="PKM27_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06525.1"
                     /translation="MKIRWLVLGLVLTTRAGSQDTIAYTQETDELVISATRSAMSFRQ
                     IAQTVKSITAKDLEYSMPQTMADALQKTGGITVQKSQQGGGSPILRGFEANRILLVVD
                     GVRLNNLIFRGGHLQNIITIDPNMLEKTEILFGPASVAYGSDALGGVIHVLSRKPALG
                     LPKAFQSSVAARYSTSNQEKMIHYEGAWSGKRWAALLSGTYSDFGDLNGGRRKNGFYD
                     HRYGYRYHYFNFENGKDVLKTNDKPWKQVGSGYWQTDWLGKIRYSSGKRTDHILNLQY
                     STSSDIPRYDRLTDPDKMTVLRNGDWYYGPQKRLLMSYSGEFKTGFPLRINLNYQQVE
                     ESRHNRRAGNPNLQSRFEKVEVLGFDLLHQSGNRVHQWIFGLDGQYESLHSSAFLENK
                     TSGTRSPLDTRYPAGRNRMMRSGLYGMFTQVISERLALQEGLRFGFTSLNSRFGTKDF
                     FDFPFDRVRQTHFTYSGNFGLSYNPGPDTKWGAVLASGFRVPNVDDLAKVFESTPGVL
                     IVPNDALRPEKSLGLEFNYTHWGKDRRSQIQISSYLTYLFDAIITSPFRYLGQDSLWY
                     NGVMSRVFANQNQNKARIAGLSLEGRTPVTDRILVQLVTAMTRGRILRKSGNTPLDHI
                     PPLSGKVGLFYEENRFNLEFNSLFNGWKRLDQYLLNSEDNEAYATPDGMPAWLTINIY
                     AGWQVSESTRLRLSLENLLDTSYRVFASGINASGRNLSVMIKTGF"
     gene            15315..16550
                     /locus_tag="PKM27_04365"
     CDS             15315..16550
                     /locus_tag="PKM27_04365"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNR06526.1"
                     /translation="MQKIVCPACNHAFHAEEVISRQLTRDLEKKFREKEESLLTDFQK
                     KMNLLEGREQELEAKIRKENTLFAERIEKEKIKIQEQIRIQQERDFRIKLDTLEKERN
                     EQAARLEALTQAAVENEQLKRKLGTLKSDLELQMQIKMTEELGKDREQIRREESRKAE
                     LRQRELEKQLDDQKKLIEDLQRKTRQGSSQAVGEAQELAIEDYLREQFPLDEIEEVRK
                     GVRGADCIQRVHTRLIPNAGTIYYESKRTKDFQAGWIEKLKEDMRLVGADLGILVTQA
                     MPKDMPHMGERQGIWICSYEDFKPLAGILRHGLIEVATALRSQENKSDKLHLLYTYLT
                     GNDFKMQIEAIVEGFKQIQDDLQKEKIAMHRLWAQREKIIDKVLLNTSQFYGSVRGIA
                     GSAIPVISVLDLPGEPESL"
     gene            16653..17201
                     /locus_tag="PKM27_04370"
     CDS             16653..17201
                     /locus_tag="PKM27_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06527.1"
                     /translation="MLNQLNELFQSANLDGKSLEFLNNAIKAQALQGFDYLKFKQSIK
                     ALQDMSMSEEMAIRSAFSTASTMGITKQKILENIQHYQGVLNKEKSEFDSALQKQTNT
                     RINQKQNETSYLQEKIKAHQAQILELEKQIKEFQFKIDNADKEIEEARQKILDTKERF
                     EETYTHYMDVLRSDIEIFNKYL"
     gene            17226..18239
                     /locus_tag="PKM27_04375"
     CDS             17226..18239
                     /locus_tag="PKM27_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06528.1"
                     /translation="MAETPFNPAPAKPKSFWKRPEGVTGTIVLLGLLAGGGFLLYKAL
                     PTLIALASNMLTLVLMLLALGAIIYMVLDPKMRALVGYAYKSIMRWVTGLFITIDPIG
                     ILKNYVEDLEDNLSKMSTQIGNLKGQIRKLTTMVEENTKEIQQNMVIASKAKELGQEN
                     QMILASRKAARLKEVNVKYDELRGKMDILYRVLTKMYQNSEVLLEDTRDQVKIKEEER
                     KAIRASHGAMRSAMAVLSGDKDKRAMFDLALENIADDVAAKVGEMERFMELSSNFMNS
                     VDLQNGVFEEEGARMLEQWEKESHLLLMSGKEKANNDYLNLGEPQKATRSSGGYEGMF
                     DAK"
     gene            complement(18298..19410)
                     /locus_tag="PKM27_04380"
     CDS             complement(18298..19410)
                     /locus_tag="PKM27_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNR06529.1"
                     /translation="MNKITFFLILLFSAIAESPAQGTLDSILPVRGICLSAPSPDGVE
                     KFTEFIRSELVPRKVNTLVFLIGYRYQFKSHPELVADNALSEMQVKKIVNACKAGGIR
                     IIPQINLLGHQSSTNKVGPLLKAYPQFNETPWIPSRQDSSTVTWPNEWGLYCFSYCPL
                     HPEVHQVVFEAMDELVEVFEADAFHAGMDEVFFLGEEKCPRCSGRDKAELFAGEVTAI
                     RNHLAKNNKELWIWGDRLIDGKATGVGFWEGSYNNTSRAIDLIPKDVLICDWHYERPD
                     PTAILFALKGFRVLTCPWRFPDVTLEQLKIMVNQRKFATPAVKNNYLGVLHTTWTGGD
                     NFIEQFYGRQPVNTRRGGNYVDAFKALFDEINKLGN"
     gene            complement(19421..20542)
                     /locus_tag="PKM27_04385"
     CDS             complement(19421..20542)
                     /locus_tag="PKM27_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNR06530.1"
                     /translation="MANLFIRTVSLFLLISLSGTATSQSVLDSVLPVRGICLSVPKPE
                     GVDRFVKFIKDELVTRRVNTLVLLVDYNYKYKSHPELVAENALSKKEVKKIVKACKAG
                     GIRIIPQINMLGHQSWFQQTGQLLKAYPQFNETPWVPMKEGSGDLKWPNEWGLYCLSY
                     CPLHPDVHKVVFKVIDELMDVYEADAFHAGMDEVFYLGDEKCPRCAGLDHAELFAGEV
                     TRLRNHLASKNRELWIWGDRLIDGKTTGIGYWEASYNNTHRAIDLIPKDVVICDWHYE
                     RPDPTAILFALKGFRVITCPWRFPDVSLEQLKIMVDSRKSASRTVKNNYQGMLHTTWT
                     GAEEFIDQFYGKKEVNTGRGGNYVEAFKVLFEEINKLDK"
     gene            20634..21047
                     /locus_tag="PKM27_04390"
     CDS             20634..21047
                     /locus_tag="PKM27_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR06531.1"
                     /translation="MKTIFFLLAGLFPVFLVSQNEHPVEYKIVSIFFSGGSYYISEEE
                     KMKVREFLANEILGNYEIHIHSHTDNIGGVRFNEWLSKMRSESTLDFLIEDGVPTNKV
                     FIKDHGLFNPEFDNSTWDGRLKNRRVDVVLWPLPF"
     gene            complement(21058..21894)
                     /gene="purU"
                     /locus_tag="PKM27_04395"
     CDS             complement(21058..21894)
                     /gene="purU"
                     /locus_tag="PKM27_04395"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNR06532.1"
                     /translation="MDKNHVVRIQCMDQKGLIARISGILFQHNHNVLVMKEFVEQSTN
                     TFFARLELTGTLNADLLSSRFRNVLPEGAEVTIIPRRKKKLVLFVTREHHCLSDLLVR
                     HHFDELHASILAVIGNHGDLGPFTERMGIPFFHIPHEEDRTVFEKQMIETLDRFDPDY
                     LVLAKFMRILSPHFVSRYQNRILNIHHSFLPAFKGANPYKRAFERGVKIIGATAHLVS
                     DDLDEGPIVVQKTIEIDHEHELRDMIEAGHEIEKAVLAEALKKIVEDKVFVSGNKTIV
                     LG"
     gene            complement(21920..23284)
                     /locus_tag="PKM27_04400"
     CDS             complement(21920..23284)
                     /locus_tag="PKM27_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNR06533.1"
                     /translation="MVNLLHLVQTNMHMIKTVGNVTFWSLAVSLGGFLFGFDTAVISG
                     GEQAIQKLWDLSDVMVGQMVAMGLYGTIIGALTGGIPAERWGRKNTLFVIGILYLICS
                     IGSAMALNVEMLMAFRFIGGLGVGASSVVAPMYISEIAPQHKRGQLTALFQFNLVLGI
                     LMAYFSNFLIGAGTGDSWRLMLGIMAIPSAIFIISLLFVPESPRWLITRRSRTEEAKQ
                     ILKTIDPGTAEASLAAIQSPHPENETSLAHFVSGKFNFPILLAFLFAFFNQWSGINAI
                     IYYAPRILEETGLGKSAALLSTVGIGVVNLIFTMLGLSLIDRYGRRFLMFIGSFGYIL
                     SLSLVSIAFFRGTGGTMVPWLLFLFIASHAIGQGAVIWVFISEIFPNRVRSWGASLGS
                     STHWLFAALIAGLFPHFNSKYGGGTIFLFFALMMVLQLLFVWKLMPETKGVSLEDLEK
                     KLVK"
     gene            23315..24796
                     /locus_tag="PKM27_04405"
     CDS             23315..24796
                     /locus_tag="PKM27_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06534.1"
                     /translation="MKTSGIKSFLSGVLQGSLVFLIHGILRAQTLQLHPVNPHYFNYK
                     NKAIVLVGSGEHYGALMNTAFDLETYFATLKKDGLNHTRVFMGAYFEKPGAFGIEKNT
                     LAPKPEQMLLPWAFQGGSYDLTLWNPAYFQRLHQLMALAGEAGVIVEVTLFSSYYGAG
                     WDYHPFHAKNNIHGLDETLDYRKAHTPDNGKLLQYQETYVRKLVAELNAYDHFYFEIQ
                     NEPWADIRDTLIVWNQNFRQEELKVAGNFWKNTIEIPSGLSLDWHRTVSGWIRDEEKN
                     RPKKHLISHNIANFKLPVPVTDDRISIYNFHYAFPESVDLNYKLNKAIGFNETGFAGR
                     LDSIYRRQAWRFMMSGGALFSHLDYSFSTDHPDGTDTLQKAPGGGSPALRKQLRFLRT
                     FVEGLNLAGLRPAPDFIYHVEGAFRWGMQDGRHYALYLEALSPNPAKVQLLLRPGNYL
                     AEWYEVNTGRKIHSEPVKAVKKWYEGKAPAGMADLVLLLKKTH"
     gene            24797..25735
                     /locus_tag="PKM27_04410"
     CDS             24797..25735
                     /locus_tag="PKM27_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06535.1"
                     /translation="MISGFTFLRNAGALYYPYVESILSILPLVDEFIVVLGEGDPGDR
                     TREKLDELRSPKLKILDSVWDTGRYRGGSIYAQQTDLAKAACRGDWLFYLQGDEVIHE
                     EDHALIRQACEAYIQDPRVEGFVFDFLHFYGDYRHYFRDHCWYKKEIRIIRNLPGIHS
                     WRDAQSFRYIPDFKDPDYFRTEGTRKLNCIALSARVFHYGWVRPPDRMALKNAEAGKN
                     YQKPGYEKFQGRFDYGRLDRARVYDGKHPSVMNDWMTQLNWQDLLRFSGPAALNRPLM
                     KHEKLRYRILHSIEERILGGRVIGGFRNYHLLQAEQ"
     gene            complement(25758..27101)
                     /locus_tag="PKM27_04415"
     CDS             complement(25758..27101)
                     /locus_tag="PKM27_04415"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNR06536.1"
                     /translation="MVTGLHDPDQGQALPVLHPACLHAEIRYFCAMKKIHFLVLFLMG
                     TNGWMTAQNPQDLLQAKIDVVYLSSDYLAGRETATEGEKLAAEYIRHRFESLGLKPAG
                     LKGGWLYPFNFQETLNPHDTAKRSLGPVKTGNNVLALLDHGAPNTIVIGAHYDHLGFG
                     GTGSGSLAANDTSIHNGADDNASGVAGLLAIAAKLKNNLKAKNNNYLFIAFSGEEKGL
                     FGSNAYTKDSTLFQAGKVNYMINLDMIGRLNHEKILAINGVGTSPSWKPRFENQAMAN
                     GMKISATESGQGPSDHTSFYLKDIPVLHFFTGQHQDYHKPSDDAHKVNYEGILQISDL
                     IIGLIEDLDSKGKLAFTKTKDESGKRAVAFKVSMGVMPDYLYQEGGMRVDGVTEGRPA
                     ALAGLQKGDIIVKMGEKAIKDIYAYMEALGSFQKGDRVHVEIKRNGEVKSLEVQF"
     gene            27157..27396
                     /locus_tag="PKM27_04420"
     CDS             27157..27396
                     /locus_tag="PKM27_04420"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNR06537.1"
                     /translation="MKRNLSNLDRWIRMGLAFLIAVLYFTNAISGTTAIILALLGIVF
                     VLTSLVNWCPLYHVLGISTYPKKGQQVSTGNPKPV"
     gene            complement(27370..28356)
                     /locus_tag="PKM27_04425"
     CDS             complement(27370..28356)
                     /locus_tag="PKM27_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNR06538.1"
                     /translation="MMESPNLQDQKTKNREARLFSVGILILLFMVSFFPACEVEFITR
                     NIDLEPEIVVEGFIEAGPEALPAYVILTRSKPFFSELSPAELDALFVRNAVVTVNDGQ
                     QTTTLDELCFNELPPDLRRLAAARLGLNPDSILVNFCVYIDLNAAIKPEAGKSYHLNI
                     VAGDQRIEASTTVPAYVPLDSLWWREAPGKPVDTLLQLMARIQDPPGQKNFYRYFCKV
                     NEGPFETPFSSVNDDILIDGKTFDFRLIRPRQEGEEFDQSTFGLFRTGDSITLKWTSI
                     DEAHFNFWNTFEFNKGNQGPFSSYTTIDTNIKGGLGVWGAYSTGLYRLRVAR"
     gene            complement(28353..30683)
                     /locus_tag="PKM27_04430"
     CDS             complement(28353..30683)
                     /locus_tag="PKM27_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06539.1"
                     /translation="MKKAFLYFLLLTLSLDLLAQQKTFTLSGYLRDSLSGETLTGANV
                     YVRDRPQIGTSSNHYGFYSLSLPAGEYAVVFSYLGFEDRIETVRLSQDQKLDILFNPG
                     IQLEELVVEENKSLEQVQSTAMGRVDLPTDQIKRMPALFGEVDVLKALQLLPGVLSAD
                     EGGAGFYVRGGGADQNLLLLDEAPVYNSGHLLGFFSVFNSDAIKHTSLIKGGMPAYYG
                     GRLSSVVDIQMKEGNARDYVVEGGLGLIASRLTVQGPLVEDKSSFIVSARRTYLLDLA
                     QPFIRKTDFAGTNYYFYDLNAKVNHRFSDKDRIYLSGYFGRDILDYRSGERDFALRMP
                     YGNNTATLRWNHVVSNKLFMNTTAVYNGYRFRLDGEQDLFSFRLNNGVRDFQLKLDTD
                     YYPAPGHFIRFGANVIHHRFTPNILSGASGETTFTNDYKPKYGLESAVFFQDEIRISE
                     RLTTNLGLRWSRFTQIGPFRDGDKVYDDFEAVKSFDGLEPRLFLTWMLNPRTSLKFGY
                     SQNQQYIHLVSNSASTLPTDVWVPSSLYVKPQRGNQWSAGWYKTLKDGTWNLSAETFY
                     KSLRNQIDYHETYTSNQSTEIEEAFVFGKGQAYGLELFVQKSRGKLTGWLGYTFTRSW
                     RWFRDIEQGRKFPASFDKPHDLEMVASYQVSRKWSLSANFVYGTGRPFTPLRSVYFID
                     QDLVTRYGPRNSARYQDYHRADFSANYTPKPDSKKAFSGSWTFSVYNLYNRKNPFFIN
                     YDLDSDLSAGRAKATAYQVTLFPLIPSVTWNFKWKS"
     gene            complement(30745..31542)
                     /locus_tag="PKM27_04435"
     CDS             complement(30745..31542)
                     /locus_tag="PKM27_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNR06540.1"
                     /translation="MSAGNALLWSIEAGPASPASYLFGTMHSASPASQQFVPRVEPYI
                     LGCSAFYGETDIGQLLETSLPAPPLEWKGLKSLLAPPSYAKARHFLEKWQGLDLDALQ
                     YLPPLFIPALIHEKYLSRSGGDPLDIRLWHLAKRAGLETGGVESLERQLEIYQKIPFR
                     IQLLQVKAQMKSLSASFRQLKRLEEAYAHEDLTHLYKLSRRQLNGMRRLMIDQRNREM
                     TAFIKNILVKGGPPVFFALGAAHLPGSKGILRMLKAAGFALRPVSLM"
     gene            complement(31545..32021)
                     /locus_tag="PKM27_04440"
     CDS             complement(31545..32021)
                     /locus_tag="PKM27_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNR06541.1"
                     /translation="MTDYKLDKLDYRILKILQENSKITNLDLSKLIGLSPAPTLERVK
                     KLEQAGIIASYHAKLDSKAMGFQVKTFVLVSLNWKSPDTMDRFLEKIRRIDEVTEVYI
                     ITGEADALIKIVCKDIPSYESILFKKLSQIEEIERLKSMVTLSTAKESMVLPLQYE"
     assembly_gap    32126..32135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(32159..33505)
                     /locus_tag="PKM27_04445"
     CDS             complement(32159..33505)
                     /locus_tag="PKM27_04445"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNR06542.1"
                     /translation="MNKKNQISFYESVSRNFDKAALHSNLDKGLLAQIKACNAVYHIT
                     FPAKIGDEYVVIEAYRVQHSHHRQPTKGGIRYSMEVNQEEVMALASLMTYKCAIVDVP
                     FGGAKGGVKINPFILKPEEIERITRRYTLELIKKNFIGPGIDVPAPDFGTSSREMAWI
                     YDTYMTFRGGELDGIACVTGKPVSQFGIRGRTEATGRGVYYGIREFCDELPEMKALKM
                     DRGVAGKTMVIQGLGNVGSYTGTICQEEGDVKVIGVSEYEGSIYDPKGISVTELLKHR
                     KETGSILNFKGAKNLPDRMSALELECDILVPAALENQVTMQNARKIKAKMIAEAANGP
                     VSSDAEELLLKKGVAIIPDMYLNAGGVTVSYFEWLKNLSHMRFGRIEKRFDQQAYGNL
                     VSMVENLTGKYIGQREKNLLVKGADEIDLVRSGLEETMVSSLQQILEIKRSKNNYT"
     gene            complement(33483..33686)
                     /locus_tag="PKM27_04450"
     CDS             complement(33483..33686)
                     /locus_tag="PKM27_04450"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06543.1"
                     /translation="MNFSIPPIFLLLMQDADFFRSTGKIYTVAVVALVIWLLVLFYLI
                     RLEKKLKSIEDQMTKHNEQEKPN"
     gene            complement(33711..34715)
                     /gene="ccsA"
                     /locus_tag="PKM27_04455"
     CDS             complement(33711..34715)
                     /gene="ccsA"
                     /locus_tag="PKM27_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNR06544.1"
                     /translation="MPYVILMGFMVPLGQGIVNINPFIVKSLNTNTLTITGYNTRFNS
                     EGKIKAWLRQGEWHLEADQVLVQDERHASLVFSIPVFPDSMQNQVDLSLVMDTPDGPL
                     VLPSAVSLSQDVAHATPGFQAAGQWSKEMLTVREESNFNYPFRNILGETIRNTYFHVP
                     LWFAMVMVFMFSVYYSYRYLRFKEIQDEWKTESLVAAGVLFGILGTVTGAIWARFTWG
                     AFWSFDVKQNMTAISLLIYGGYFLIRSNLGDPDARARISSIYNIFAFVLLIPLIFIIP
                     RRFDSLHPGSGGNPAFGSQDLDNTMRLVFYPAVIAYICIAFWISQIRYRIKLLYNKID
                     "
     gene            complement(34766..35419)
                     /locus_tag="PKM27_04460"
     CDS             complement(34766..35419)
                     /locus_tag="PKM27_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNR06545.1"
                     /translation="MLREVFGLLGKDFKSELRNKQALTSISLYLFSIILVLYYSLMRV
                     ERMTWTGLLWIVLIFLSINTVQGCFSREVKQRHWYIYTLARPLSVYFSKVLYNLILLL
                     GLNALAILLIGLFFTLPVTHWPLFILTMVLSTSGISLIFTFISLITAKSGDQAVVMTI
                     LATPLIFPILMAGSRLCLVAIELIQDSNYYKDLLSIFAMNLITFALSVFLFPILWRD"
     gene            35555..36925
                     /locus_tag="PKM27_04465"
     CDS             35555..36925
                     /locus_tag="PKM27_04465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNR06546.1"
                     /translation="MKQCIYPKGKAQIIGIFMLLSAYSPLIAENYISVKIKSGDGLYS
                     LFRRYQLPANSCNINQFKQINQLKDINKLLLDRTYRLPVLLMPFNGKSVSTSVPGIDK
                     STVVRIINYNKTVYTNKLRKLPYNQSKLVWIPYHELNCSSGSAMKSSSASVRTTLAVS
                     SAKPASAVKPAASPSVKSVNSKPETVKVENDLESTAETTSNLEPAVSEKAGLRTRTVP
                     LFGPDFKEIRIESELLKNKVFYLVSGHGGPDPGTMYQGQNMTLCEDEYAYDVTLRLAR
                     KLMERGATVHMIVQDPNDGIRNNMYLPMDKDEKCITGYPLVLNQRLRLTQTTDAVNDL
                     YRMHRKQGIAEKDQVAIHIHVDSQHKDLRKDVYFYYQEDNKHSLEIARKIQKTLDNKY
                     QDLAGRSYNGTISSRNLFVMRNTIPSTIFMELGNIQNTMDHKRLLISSNRQALAEWIY
                     DGIAGE"
     gene            complement(36954..37934)
                     /locus_tag="PKM27_04470"
     CDS             complement(36954..37934)
                     /locus_tag="PKM27_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HNR06547.1"
                     /translation="MHWETERKGIMDLIRLGGFVFDELALRLYEAQYAFNPVYRAYLD
                     LLYGSAPAVNTCEEIPCLPIEFFKTKEVRTGSYNPSLVFESSATTGQQRSRHFLREPG
                     WYHEMASVCFSHIFGQQPGQFRHLAVLPSYAENQASSLLYMLDQFMKRGQGGYYHSDP
                     AGLSVELEKTIKEPTILWGVSYALYNWPMRLQLSGDTLVMETGGMKGREKEMTRQELH
                     QKIMENFGVTSVCSEYGMTELMSQAYALSEGLFVPGPCMRVVPRQINDPLSREQPGQQ
                     AALNIIDLANVDSCAFIATDDLGRVYGDGRFEVLGRLDRSDIRGCNLLLA"
     gene            38008..38502
                     /locus_tag="PKM27_04475"
     CDS             38008..38502
                     /locus_tag="PKM27_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06548.1"
                     /translation="MMGKRILFLTLFLVVVLIQACIIAPDYPIIPEISFEGVNKTVVD
                     QGRARDDSLYIRIAFTDGDGDIGDEQNKANIFMEDDRDGMLVTYSMPLIPQKGVANGV
                     SGTITILHTTLFNVCCYYPNADPCSIPDKPTTDTVTYSIYIEDRAGHKSNVIKMQPIV
                     INCK"
     gene            complement(38513..39652)
                     /locus_tag="PKM27_04480"
     CDS             complement(38513..39652)
                     /locus_tag="PKM27_04480"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNR06549.1"
                     /translation="MNETRQIVIAGAGGIGRAVALMLADHYGDGLRVFIGDQNPRACE
                     SALQWLRSGQVNTEQVKTFSMPAAGSNEEMDSILRSSSIVLDCLPGTQAPRIARLARQ
                     FNLHYVNLTEYVKETEEVIRIAQGADTAFVLQTGLAPGFIDILAHRLYLDLFREYPVE
                     KADKIVMRVGALPLNCRDPHFYGFTWSPVGVATEYLEDCVVIRNYQKTLVPALSSRER
                     LIIDGIHFEADLTSGGAADLPDFFKGKLAELDYKTLRFPGHYDWVKISASTIPQNRNK
                     IDELQRIMLENIPMVEEDFVLIYSSAQGRDANNTLWLNERSYWINQINWGELKLKAIQ
                     ATTAAPMAEIARMLLTENWKGPVFQSQIDPYRFMEGPFVTRVYAK"
     gene            39750..42638
                     /locus_tag="PKM27_04485"
     CDS             39750..42638
                     /locus_tag="PKM27_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNR06550.1"
                     /translation="MNEENLKALLASLEGSLSWDETNRTIYATDASVYRKLPAAIALP
                     KSVDDLKKLIRFAHENKTSLIPRTAGTSLAGQCVGEGIVVDFSAHLNQVLEINKEEKW
                     VRVQPGMVRDKLNQVLAPLALFFPPETATANRAMIGGMVGNNSCGANSIVYGTTRDYV
                     LEIKALLSDGSEAVFRSIRPEEVNRKTGLEKKVYDHVLDELGHEETVHEIRSQFPKKN
                     IHRRNTGYAVDMVLNELEKGQVDLTRLICGSEGTLVMVTELTLKLVDPPPPFNLIVVP
                     HFHSIHECMKAVVAIMKHRPFACELMDRVILKCTDGHEVYKTYKFFVDGDPEALLMVE
                     FRGNHQEEIEHQARELIEELKSLSFGYAWPIVRSPETKKVWDLRKAGLGLLANMEGKA
                     RAIECIEDTAVALEDLPAYIEEFAALMETYGQKPLYYAHAGAGELHLRPVLDMHDPEE
                     VKEFRAICEDSARLVKKYGGSLSGEHGDGRLRGEFIPLVMGQKNYELFIRIKETWDPH
                     YIFNPGKIVHAPRIDEDLRYTYERAGKEPETQFDFSGQGGFFHASARCSGSADCRKQA
                     SMGGTMCPSFMATRLEKDTTRARANALTAFYSDPSLSSIRDMDSVKEILDLCLSCKGC
                     RNECPSNVDMTLLKAEFTHQYRMKKGLPWRSMLFGGIDHINSVAGLMPVLANKIQNNR
                     ILKKWIGIHPDRSLPALGSHSLRQWTSRFKPSASSGKQPVLFFIDEFTNRYDVQAGIK
                     AILLLDRLGFPVRFAKHRSSGRAQFSKGLLPEAKKLAAWNLRVLSEFIPTHQVVGLEP
                     SAILSFRDEFPKLFRGAEQAKAEALAAHCFTIEEFLFHEMQKGNISGQDFDQTPRILL
                     IHGHCHQKALSKMEYTSFLLGLPAGHSVSTIASGCCGMAGSFGYETEHYSLSMQIGEL
                     VLFPAVRAASPETRVVAAGTSCRHQILDGTARKAFHPVEILWEALKSA"
     gene            complement(42640..47418)
                     /locus_tag="PKM27_04490"
     CDS             complement(42640..47418)
                     /locus_tag="PKM27_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNR06551.1"
                     /translation="MSIPEKYKATFKGKIFRGLLTLVVGCILLILFLALVIQAPPVQN
                     WARTKAEVYLSGKLGQAVTIDRFSLEWFNRFMVSGVHVSDARQDTLVHIDRFAVDLNM
                     AITSLLRKDFRFKGAFLEGLTLKDLQYEGDSLSSLEQALRKLNLKSGGKKSGGGGISL
                     DLESLALRRIHFIQDSPNSTSDIYLDDAAINVDLLDLKNKRFEIDRIVLTRPSISLSS
                     KGPKTKSKAFKWIEDSLLTVKIRELSIRDGRFDRYDIKPVVSWQAVTNHLQDISLEVE
                     SLSLSQGRLEGTLGKLTALNPQQMGIRNLSAGSIVVDYQTIQLNGLDLQTRDSRLKDS
                     IQLHFKGFENFARFEDEVKWKMVLNGSSLSVSELLEWLPSLKKNRFLQANSAKKLEMD
                     GLLTGSINDLKGSKVVLKIGNTLSLNGDFDLNNVTIPGESFLSLRLKNLQTDMASLRQ
                     LLPANEKLANFDKLGKLRFRGNFDGSLADFVAYGVLQTDIGSSRMDMRLNTRKGAEVA
                     SYSGEIDLIDFDLGKWAENEELGKTSLKAKITDGKGLTAATAEATLEAVVSQLDFKKY
                     AYKDITFDGHLNQKLLDGKLILRDANADLAFIGKIDFTNTQPAYNFTAEISQLDLYRL
                     NISKKPLAFSGNTEISMIGRNFDELIGKVDLDQVIIQVDSVPVLLQNMEVDADRSADG
                     TRGITVHSDWIDGELRGNYLIKNIWPALNQQLKNQFPDFLKSLPIQEALKQNPDSVIS
                     CSYDFTLDVKDLRGLSRILKQDMEASQPFRLKGSVHSAQKYMVANWNIVDFRWKDFKV
                     FNAIGRLEAKGAVAYTTSLIDSIYSGNFRIPQLVLTADLSYNLLNFSIRTPEVSKLLN
                     NVSLNGAIRLIDSSWHLSLAPSNLSFMDKNWSILPNNEIVYRPGYLRTQNLRFEHGTE
                     RIEIVSRDNKGLRVQFTDFDINWVNSFNPVKAWDAGGRLDLNAEIADVFTFRGVRLSG
                     RVDSFYINDTYFGLLDLNLYNVNTNQPFNLSVALLDGSRQLMGEGFFDPSGKNSNGVK
                     NNYQFKVLFKDFPLKLFEFMIDDIVDQTRGNIVGTFEINKVGGKPEFKGRVNIGDGSL
                     KINYLGTSFQIGNQPVTITNNLIDASGVSLRDELGNTGTIQGGIEHDRFNRFRLNAGI
                     RSNRFLVLKTTKVQNPDYYGTVVGNVNARFTGPFNRIDIDVQGTTARPTVLYIPISQT
                     TEITSERLVKFRPVQASTASETQEPRVVAANGLSVSIDLTVNDDAEISLIFDEKRGDI
                     LKGRGNGDLQMRFERNGDIAMFGNYEVEQGEYLFTLLGVVNKPFVIRQGGTIRWDGSP
                     TDADINLDADYKGLTSSLINLLPEYENAIESAELRTQSAVDLSMHLFGKLFKPEISFG
                     LDIPNLTGNLRNIVDNKLNLLKSDQNALNQQVLGLMVWGSFLPPNQLVASSGVIGSTI
                     NNLSQFISNQLSILVENALKELVADNAVISGFDFDVNYYNNNNAIDINNLSVFDEVAI
                     SLGPRFFEDRLSVGVGANFINSTLFNRLITPHFEVEYALTRDRRLKIRAYAKKDDINQ
                     GQLRDRIGGGLSWRKEFDSIREFKQKLKDDLNQRGPASDL"
     gene            complement(47487..48785)
                     /locus_tag="PKM27_04495"
     CDS             complement(47487..48785)
                     /locus_tag="PKM27_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR06552.1"
                     /translation="MKLKNTIGLVLHSCLIFVCTVQLYSQTIYLHCGNLVNVNSGKVE
                     PGMTLVVEQKKIREVLHGYVDPPASAKLIDLKDKTVMPGWIDMHVHIESVIERGSYIN
                     RFTLSESDVAYLAASFGEKTLLAGFTTVRELGGTGVNVAYKNAILKGLATGPRIYTSF
                     KAISSTGGHGDQTSGAKKGIYNAPGPESGVADSPDECRKAVRQMLKDGADVIKVTATG
                     GVTSLTRDGSRPQFSQEELNAIVETARDYGVHVAAHAHGDEGIRRAILAGVTTIEHGS
                     QMTDSTMDLLVRKGVYYVPTLTAGMSVSDSARIPGFFPEMVRVKAIETGRLIRNVFEK
                     LVKKGARIAFGTDAGVFPHGKNGLEFQYMHELGMSPMDCIRAATVINASILGISDQTG
                     SLEAGKFADIVAVEGDAMRDFNRIQQVRFVMKEGRIYRHD"
     gene            complement(48797..49693)
                     /gene="folP"
                     /locus_tag="PKM27_04500"
     CDS             complement(48797..49693)
                     /gene="folP"
                     /locus_tag="PKM27_04500"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNR06553.1"
                     /translation="MNILTTFVLSMYTDPFFVPPSLNCRGTLVSLERPVILGILNLTT
                     DSFYKGSRAIREDEILEKAGRMISEGARFIDLGAMSTRPGAPELEEEVEKERLHLACS
                     CIRSRFPEVLLSIDTYRVSVLEAIAPYGIHLVNDVSGGAGGEIWDWAAQYKLPYILMH
                     MKGTPRTMNSLVQYDDLIREVLDYFIQRIPLLLKKGVHDLILDPGFGFAKTVDQNYVL
                     LKNLHVFKLLEFPILVGLSRKSMIWKTLGVTPEDALHGTTALHIQALNAGARLLRVHD
                     VRAANDTIKVWEKLQGSAPENS"
     gene            49937..51277
                     /locus_tag="PKM27_04505"
     CDS             49937..51277
                     /locus_tag="PKM27_04505"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNR06554.1"
                     /translation="MRISHRQNQGDRFASSHTRYGIFLFCSFLLFSSFFLFPARFGKL
                     SAPPAIQEEQCVEPVYLYGFDSNIFHIEEGKILRNDYLGKILSNCGIPGDLIHQISEH
                     AKDIFSVNKLQAGKKFALVSGQPCGKPDYFVYEPNPWSFVIYGLGEKPEAKVVQRPIV
                     TSTHFKEGHIESSLWNAMADQGSTLQLISLMEEALGSQVDFYHVQQGDSFRLIYETRA
                     VEGEIVDIGKLTGAYFKNENKEYYAIYYETSKHKGFFDLKGAPTKKSFLKSPVEIGYI
                     SSRFNPSRLHPILKYRRPHLGTDYAARHGSPIRAVANGTVTEAQRKGGNGLYVKIRHD
                     KTYETQYLHMSKLGSGIRPGTRVNQGQTIGYVGQSGLATGPHVCFRFWKNGRQVNHLR
                     ENFPDPDPMPKEDLPAFFQWRDKIMDQLRSGNSLDAQNHPDFTDYPRPIPLIKS"
     gene            complement(51299..52348)
                     /locus_tag="PKM27_04510"
     CDS             complement(51299..52348)
                     /locus_tag="PKM27_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR06555.1"
                     /translation="MKNITPRQLAILTGAVVCAAVYFNQWFFTQILYSEQQNILRLVI
                     ALFTGLMAYGVTRFIINKYLVPKVRHIYEMIHRPSSTVDAPTQIYPDKPLFQQVEKEV
                     SQFIKSQNKEMSALRDLERYRQDFVGNVAHELKTPIFNIQGYVHTLLDGALTDPKVNQ
                     LYLRKTAENTDRLIKIIEDLDSIYKLESDQLSLEWEEIEMIGFTEAMLHEMELMAAEK
                     KISLDLKTETDHEIWVKADPDYLRQVFVNLLENSIKYGNPGGHTTISLHDLGEQVLVE
                     VADDGPGIEARHLRHIFDRFYRVDKARSRQAGGSGLGLSIVKHIVEAHGQQIKVRSSP
                     GKGTVFSFTLAGKVE"
     gene            complement(52354..53028)
                     /locus_tag="PKM27_04515"
     CDS             complement(52354..53028)
                     /locus_tag="PKM27_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR06556.1"
                     /translation="METKAKVLVVDDEDDIREFLAYNLSKNGYQVEVAADGLEALKLA
                     ERFNPHIFVLDIMMPGMSGVELCRKIRAKPEFKDVLITMLTARQEDQTQIEALNVGAD
                     DYITKPLKPSVFLSRINALLRRSDIKTNESLKFEDLVIDPSSFTVSIKGTPVDLPKKE
                     FEILHLLASKPGKVFGRNEILAKIWGREVIVGDRTIDVHIRKLREKIGDKYVQTIKGV
                     GYKFVF"
     gene            complement(53120..53464)
                     /locus_tag="PKM27_04520"
     CDS             complement(53120..53464)
                     /locus_tag="PKM27_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNR06557.1"
                     /translation="MKAMIRLAFLISLTCPAGSCGAHERKLSKEEYRDIILDLQVAET
                     IILNSVLTNKDSLRFVYQQRICEIYGFKDIASLKQSLQPLEADPELMLEITKEMTKRL
                     DQLEDSSNLSFE"
     gene            complement(53448..53957)
                     /locus_tag="PKM27_04525"
     CDS             complement(53448..53957)
                     /locus_tag="PKM27_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNR06558.1"
                     /translation="MKTVCFIRHAKSSWDQAGLADIDRPLNLRGKVTAPLMAKILAER
                     GFKPDLIISSTAKRARQTAKYFRKQYGIKKEEMQVEPLIYGGDEQAIFDILRKLPDTV
                     DTVFLFGHNPTMNYLAALFSEDHIHVPTCGILVVQLNIHSWKDVQPDHTALKHFIYPK
                     QFMPDEGDD"
     gene            54420..57584
                     /locus_tag="PKM27_04530"
     CDS             54420..57584
                     /locus_tag="PKM27_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06559.1"
                     /translation="MKKRFALLILLHLSLGAALAQRTVTGTVSDDQGELLIGASVQVK
                     GTTTGTLTDVDGRFSIQVPEENTVLIFSYVGFERMEIVVGNQSHIEVILQSASQLQEI
                     IVVGYGTQKKADLTGSVASVDVPSMLTRPAADLTQMFQGRVAGVVSSGSNQPGGNGYV
                     RIRGISSFGSNEPLVIIDGVQTNGTNSLNPNDIESVNFLKDASSAAIYGARGAGGVII
                     ITTKKGVPNKTRITYDGYYGVSKVIRYPDMVNTAELGQLLWKQAAGAGIAPSSPQYGK
                     GASPVIPDYILAGSSGGLLEGNPAVDPSKYVYDQNGFYQIVRANKEGTDWFKELTQTA
                     PAQSHNLSASGGSDRAIYNLSLGYYSEDGLQKYTFYDRYSARANSEFKLGKRIRFGET
                     LFGSFRKRKGSADNGEGAPWSHAYRMQAIVPVYDIMGNFAGSKAPGTGNGANPVAILY
                     RERNNTEKDIRILGSVYAEADLITGLLFRSNYGIDFNNNFSKAFRDVNPEHSEGNFQT
                     TYSLQSGYQFRSTFTNTLNYAKQIGNHSFNVLAGTEIVEYKNEQLGGNRSGYYPFTDE
                     SFWVLDRGNPVGQGNFSSLGEEALFSVFGRIDYAFAGKYLLNATLRRDGSSKFAPDVR
                     YGNFPSISAGWKISQENFMQDIRFISSLKLRAGYGVVGNDQIDGNNQFSFYRSDPARS
                     FYDITGTNTSTVSGYDLDRKGNLNSKWEETSTLNLGLDLALFDHRFEMNLDLYNKKTS
                     DLLVQIPRPGAEGDFTAPFVNVGNSQNKGIDLMLTYRSKAAAKWYYAASVNMTAYKNK
                     VVSSGVDFFTNTTRNSPVSRTLTGQAIGMFYGYETNGFFNSGEEVAAAPTQPGINKNS
                     PQQAVGRWKLKDQDGNGAVNANDRTFLGSPHPKFQAGFNVDVAYKNFDLNVFTFWNYG
                     NQIYNNTKWWTDMNGFTGNRSRRMLYDSWTPENMNASLPKLDANDNVSNSVPHSYFVE
                     SGSYFRAKTVQLGYTLNEGFINRVGASSLRIYLQGQNLFTITEYSGPDPDLLDVGRGD
                     IGLGVDHGRVPNPRQFLAGINLVF"
     gene            57628..59367
                     /locus_tag="PKM27_04535"
     CDS             57628..59367
                     /locus_tag="PKM27_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR06560.1"
                     /translation="MKNHKIYFLLAVSMLFTNACVDKDFLDRPPVGVFSEDALKTKAG
                     INGILIGAYAGLNGSMYGNGSSAGADVNTFSNWVFGSLTSDDGQKGADVGGSRSNIER
                     YELTADNSKLNDRWAFCYDGVARCNDVIRLTPSVEGLSDTEIKNLLGQARTLRGLFYF
                     HATVIFGRLPWIDEKTTDYYQKNERLLWPEMEADLKFGFDNLPETFAELGRVNKWAAG
                     VMLAKVYMYQKKFTEAKALLDMIISSGKTTKGIKYALMAKYHDNFKIETQGSNTDSES
                     VLDAQYSVNDNGRGFNGGLGENLNFPHGGLPTQPGGCCGYHQPSQDLVNAYQTKDGLP
                     YLDNHNAQEVKNDQGIESSAPFTPHTGPLDPRLDWTVGRRGIPYLDWGPHPGKAWIRD
                     QAYAGPFSPKKNVYYKANEGTLTEGTSRQLSANNTRIIRFADVILLAAEAEVELGNLE
                     KGREYVNQIRRRAANPDGFVKNPDGTPAANYVIAEYTAPWTDKAMALKAVRFERRLEL
                     GMEGQRFFDLVRWGIAKETLNAYLAYEVTKRANLKGAAFKDHNILFPVPQRQIDATKL
                     PDGTVTLTQNPGY"
BASE COUNT        14913 a        15129 c        15074 g        14371 t
ORIGIN      
        1 cgacatccag cttaaacaaa tgccgggacc ggtatttgag gatattaaaa tccgccgagg
       61 accgccggtt gtcttcttcc gtaaaatgga caaactttgg gttaatatag gtgtaccccc
      121 ctaggacgga ccaacggtgc ctgccggtgg aacccgcacc cagactcagc tcataaccct
      181 tgatgtcggt gttgccaatg ttctgggact gaaacccttt gacaaagccg gtaaaaacaa
      241 attccatcat atcccggtac ctggaccaga acaaggcccc gtccacaaag ccggtcagcc
      301 ggtttatccg gaaaccctgt ttggctccga tctccgcagt ccagccggtc tcacttttca
      361 agcctgggtt gggggaaata aatgtggagc caaaactagt ggtgataaat tgttcggtca
      421 gggtgggaaa acggaaaccc tggcccaggg aagcccggag aaaagtccct tctcccacct
      481 tcgtgttcag ccccagccgg aaaagatttt tatggtccct ttttttgccc cccaaaaggg
      541 tatcgccggc caggacctcg ggccggctga ggcggtagtc ctcccaacgc cagcccagcg
      601 atacggtggt accggacccg agtttttttt ctgcctggat ataagcggca gcctggttcg
      661 atttcaacgg tagaaggtta agtaaggggc tctcactcct gttgtgggcg tacaccagtc
      721 ccgccgtcgt gaccagttta agaccggtcc aggccttttg gaaccggtat tcaccgtacc
      781 agagcgagga actgttggat tggccgctgg aaacggaatt gtcgaccaca tagaccctgg
      841 acaaaaaact atgcttgttt cccctttgat caaaatagtg gacgtaggga tcgacatggt
      901 acctgaatga attggattga ctcagcgtga gggaatccgg acggtaaaac gcatggaccc
      961 cgtctttcca ataaaaaaag ctgttacctt cccggttgtt tatattaaaa tgagttccgg
     1021 ctaccaggcg gtcggtgatc ctgtattgca cactgccgtt gaccctttga taatcggatc
     1081 gggaagtctg gttataactt ttgttgttca gggcataaag gcccccgacc aggtcccatt
     1141 ttccggcctt gcgcgcatgg ctcagggaca ttccggccgt ataaggcgct gaagaccacc
     1201 attgctggga tttgtccttt ggggccatca cggggttgta aaacagggaa gcccgggtga
     1261 ggggctccgt ccttgcgaaa gcggtcctta catggaccag gccgttgagt gcggccgaac
     1321 catatattgc cgaagcggct cccttaatca cttcaatctg ttcaatattt tctaccggga
     1381 ggtcgtccca gttgggataa ccagcatcgg cctgcagcgc ggggatatca ttgaccatca
     1441 acaatacccg gctgcctgcc ccgtaactaa agcctgaacc cccgcgtata ttggcctggt
     1501 cgccaatcat gttgagcccg ggtaccttat ccaggacttc atccacagaa gtggaattga
     1561 ggtgatcgat caggcctttt ttgatcagtt cgagggagac cgtgctctcc cccagcggtt
     1621 tttggtagcg ggaatcgctg atcaccgcgg tctcaaggat cccgataagg gcttccagcc
     1681 tgatatccat ttcggcccag cctgagggcg gaattgttat gtccaccgac ctggtcttga
     1741 atccaatgta ggaaatggtc accaggtagt ttccgggttc caaagccaac tggaaatgcc
     1801 cttcaaaatc ggtaaaaatg cctcgttcac cgatccggac ggttgccccg gccaggccat
     1861 tgcccgtttc ctgttccatc acagtccctc tgaaaccggt ctgggcatac aagcctgaaa
     1921 gcaaaaccag gttgaaaagg atcacgaaca agccgggagg cgtggcctta aatcgcctgc
     1981 gttgtatctt cgcaaaacga atcagatggt aaacttcaaa aaatttcagg gatgaaaaaa
     2041 tcatgggtcc tttttattct tgcctctttc caaatgtaca atatccgggc gcaaaacccc
     2101 gcctgccttc cggaccccgt gtacaaagac agtacggcag gcatatatcc gcggccttat
     2161 aacgacagta ccatgacggg gggcatcacc cggcctgttt gtatcggcca ggattatgaa
     2221 tatcccctca cggtcaatat tcccgatctg attacggtcc cctttggcaa tacgaccatc
     2281 accataggtc ttgaaagcgc catcctggat acctctaatg cggtatccgg cctgcctaaa
     2341 gggatccggt atcattgtaa cccgggcaat tgtatcatgg caaaaaacca gttgggctgt
     2401 gtcgtgctga aaggaagggc cacggaggaa aataccccgg gcacttatga tctggttata
     2461 aatctaaaac tcatcaccca ggcaggctct ttcgatgtgg cctttcccgg gccatttttt
     2521 cccggtaagt attttctgac ggtcaccgaa cccggatccc aggcctgcct gaccagcccg
     2581 gccagggaac ttcaggcgga ccaggtgagc caccgtgcct ttcccgttcc ggccgacgat
     2641 caattaaata taagagtgca tgccttccag cctggacagg gtcatctgat cctccgggac
     2701 ctctctggaa ggacgctttc tgatatgccg atgagtctgg acaaggggga gaattcattt
     2761 tccatcccgg tcagccacct tcaaaacggg acttactggt atactttaca aataggccaa
     2821 tcggtaaata cccggcgttt ttcggtcttg cattgaggat tattcctctt catccccgtt
     2881 ttcctggtct tcgtattcca ccgggttgat catgggcagc ttcacccatt ttgattcttc
     2941 ggggtttacc gccatgaagc caaggaatcc accggaagct ttggctattt cctgggcaaa
     3001 gcgtacaaag ccccggtaca ggaaggcacc ggccttgggg tgcagctttg caaggatggg
     3061 gttgagttga gccagttttc cggatatggt ttctccgcgc agcgcggcat cttcaggcag
     3121 ttccgaattg aattttgcca gtaaaggctc aaattgttct tcgacctttt cgtaaaaggc
     3181 ctggaggtat tccggggagt tataggtccg gatatgggtt atttttttgg ccagatccag
     3241 ttctttgaca tccagcgtat tatccgcttt tccgatcagg atggcaataa ggggaagggc
     3301 gtttgtcagc gattgaacct cgttttcatt caaagatttg agagcattgg acatatcatc
     3361 aattttatta atattcgggg taaagataag cacgaattga ttaatttctg ctgaacaggg
     3421 acaagtgaaa gagccggccg ctccgggctt tagaacggat ttgggagcaa ctgtttaaat
     3481 ttgcccaaaa gcagggattg gccagaccat cgaaaaatac aaagtccgtt gccagggaag
     3541 aagggaccac taccccattg ctgagccagt atgcccaggt caaaagcaag tatcccgacg
     3601 ccatcctgct gttcagggtt ggcgactttt atgaaacttt tggtgaagat gccatcgtgg
     3661 ccgcccgggt gcttgggatc attcaaacca aaagaaacaa cggggctacg gtcacggaac
     3721 tcgcgggctt cccttaccat gccctgaacc tgtacctgcc caaacttata aaagccggat
     3781 accgggtagc gatctgcgag caaatggaaa aaccagtccc tggaagggtg gttcgcaggg
     3841 cggtgacgga aatgatcact cccggtgtga tcacggggga ccagtttctt gaccagaaaa
     3901 aaaataattt cctgggctgc ctgtattttg gaaacaaatc caggcacggt ctggccttca
     3961 tcgatatctc caccggggaa tttctggtgg ctgaaggcga cacttcctat gttgaaaaac
     4021 tgctccaggg atttaatccg gccgaaatca ttctggccag aaacagtgca cggttatttg
     4081 aatccacctg gggcaatgcc tattacacct atccggtcga tgactggatc tttcaacatg
     4141 cgtacgccag ggaaaaattg ctggaacaat ttgaagtgca cagcctcaaa gggtttggca
     4201 tcgaggacat ggaccttgcc caggtggctg ccggagctgc tttgcattac ctcaaaacga
     4261 ctgaaaacca aatcaggcat atccgcaata ttcaacgcat accggaatcc gagtacgtct
     4321 ggctcgaccg cttcaccatc cggaacctgg aattgctgga aagccccctt gacaaaggaa
     4381 aatccttttt acaggttatg gaccagacct tgtcccccat gggttcccgg ttgatgtata
     4441 aatgggtgat gtttcctctg ctggatctca ggtcgattaa ccaaagactg gatgccgtga
     4501 cctattttca ccggcaatac gatcagctgg accgctatgg atccctgatc aggcaggtgg
     4561 gagacctgga aaggctcatc aacagggcgg ccatggaccg gatcagcccg agagaaaccg
     4621 tccagttgcg ccgcgccctg gaagcccttc cccccttcaa agacttgctg gtctcctcgg
     4681 gaaacgaaga gttgaggcac cacggcgaag cccttcaatt atgcgccgaa ctaaggacga
     4741 acattgaaga gaagatcaag gaggatgcgc cggctctgac caataaaggc ggcattttca
     4801 aggagggttg tgatgccgcg ctggatgaac accgctatgt ggtgcagcat gcccaggaac
     4861 ttctgctcca acttcaacaa caggaatatg aaaagaccgg gatcccgctc aaggtgggct
     4921 tcaacagcgt cttcggttat tacctcgaag tgaccaaccg gtacaaggac caggtcccgc
     4981 cgggctgggt aaggaaacag accctggcaa acggggaacg attcgtaacc gatgaattga
     5041 aaaaactgga aaaccagatc ctgggcgcgg aagaaaaaat cattgacctg gaggaaagtc
     5101 ttttccggga cctggtgaac ctgatcaagg agtacgtcca tcccatccaa cacgatgccc
     5161 aactcattgc ccgggtcgat tgcctgatgt cctttgcccg cctggcctat cagaatcaat
     5221 accaccgtcc ggtgctggac gacagttata tcatcgatat ccggcaaggc aggcatcccg
     5281 taatcgaaaa aaaccttcct cccggggaaa cctatgtgcc gaacgatctt tatctggaca
     5341 atgaaaacca gcagatcctg atgatcaccg ggcccaatat gtcaggaaaa tcggccattt
     5401 tgcgccaaac cgccctgatc tgcctgatgg cccaaatggg tagttttgtc ccggctgaat
     5461 cggcgatcat cggcatcata gacaagattt ttacccgggt aggcgcctcg gacaatatct
     5521 caagcggaga gtccacgttc atggtggaaa tgaacgaaac ggccctcatc ctcaacaata
     5581 cctccgcccg aagcctgatc ctgttggatg aaatcgggag aggcacgagt acttacgacg
     5641 ggatatccat tgcctgggcc atcgcggaat tcctccacga tagccctgcc aggcccaaaa
     5701 ccctttttgc cacccattac catgagctga acgaactggc cgataaacac cctaggatca
     5761 agaacttcca tgtgtccacc aaggaagtcg gcaacaaggt catatttctc cggctgctca
     5821 aagaaggcgg ggtccggcac agctttggga tccatgtcgc ccgtatggcg ggaatgcccc
     5881 agagtgtggt cagccgggcg cataatattc tcaaacacct ggagcaaaaa tccattgagg
     5941 ctgcgggtcc tgatacaaaa acggccgata agcttaaagg catgcccgcc cccttccaac
     6001 tcaatatatt tgaaagttcc gacccaaggg cgcaggccgt gatcaggatg cttgaagaaa
     6061 ccgatatcga ccggctcagc cccatcgaaa gcctgatgaa gctcaatgaa atcaagggga
     6121 tgctgaaaga aaattaattt cccgcttttt gcctgatctc atagcgtgta tcttcgggta
     6181 cacactatga tcaccttcac ttcgcgggga ctggttcccc tattcattct atctgcatgg
     6241 cccgcacctt ccttcctgct atatgcacaa caggacagcg cttcctggaa gcccacaacc
     6301 cttccttttg aagaggctct tcaatccctt gccgaacatg gtgaaaatcc ggacgataca
     6361 gacctgggtt tagctacaga acttcttgaa tcatggagaa aaaagcctct gaatatcaat
     6421 gacccggacc atccggatct tcaggatttc ctcttgcttg atccggtccg gctggaagct
     6481 ctcaggcatt atatccgcag ccaagggcct ctgatcagcc tttacgaact gcaggtggtg
     6541 cccggtttcg ataccgaact gatcaggcgg atcagccctt tcatccagct tcggcccgac
     6601 ctggaccgat tcaccatcag tcttcgggac cagtttctct ccagccagca tcaatggttg
     6661 atcagactga gccgcaattt agaaccgttg actggttcag gtaccgccgc agggactgga
     6721 tttacaggca gcccattggc tctgttggtc cgattcaggc atttttatga aaaccgtatg
     6781 agcatcggct tcctgggtga aaaagaccct ggggaaactt ttttccgaaa agacaacaag
     6841 gcgggtttcg accattattc tttccacgca gggatccggc aggtcggcag aattcttgaa
     6901 gagctggtgg tgggtgatta cacggtaagc cttggccagg gtttgttgat gcatgccggc
     6961 atggggggaa ggaagggggc cttggttaca gggatcagga aaccggaccg cgtgttcaaa
     7021 ccgtataccg cggccggtga gtttaattat tttcgagggc tttgcgccct attaaaaccg
     7081 aaaccccctt ttcagctggc cgttttttat tccaaccgtc aaaaggatgc caataccggg
     7141 tccgcttcca tgcagggact tggagtgatc accggttttc tggaaagcgg atatcaccgg
     7201 agccctgccg aactggaaga tgaaaaaaga gtgaacgaac aaatggcggg cggttcggtc
     7261 aggcatcgct ggagatcggg ccatctcgcc atcaatctgg tttcgagccg ctatgacaag
     7321 tttttcaaaa agaaggaatc ggcttacacg gctttctctt tttcagggca taagttgacc
     7381 caggccagcc tggactgggg aataaaattc aggcatttca attggtttag cgaaacggcg
     7441 ttcagcagcc cggccgggat cgccattgtc tccggcttgc agaccagcct cgacaaaaga
     7501 ctggatctag cccttcttta ccgcaggtat gataaaaaat accactcctt taatgccaac
     7561 gcctttggcg aatttgcgga tccggtcaac gaagagggtt tttatgcagg ccttcaattc
     7621 gcaccggcat ccagatggaa aatcaacgcc tattgggatt tctgggtccg gccctggtac
     7681 agttatcagg ccgacggtcc gtcggccggc aacgaacaac tcgtccgggt ccaatatgcc
     7741 gtgaggtata aaatggattc ttaccttcag ttgcggttca ggtcgggtga ggaaaacggt
     7801 atggaacctt ccgttttaac tccgctcctt gaacgaagac aggcgcaaat caggatccat
     7861 ctgaatttat tccacgggaa aggcctggaa acacgaagcc gcatcgaatt caacaggaat
     7921 aagctggccg gagcaaaggc gtccacgggt ttcctggctt ttcaggatct tttatatcaa
     7981 ccggtgcagt ccgtgttttc cggaacagcc agattctgct ttttttcgac cagggactat
     8041 gattcacgaa tttacgccta tgaaaacgac ctcctgtaca actatagtgt ccccgcattc
     8101 agcggaaagg gcttgagact gtatttgaac cagcgatggg ccctgtccaa acaactggtt
     8161 ctggaaggaa ggtgggcctg cacttttaaa tttaaaagcc gcttgaaaga tcccgattcc
     8221 tatgaaaatg aaggaaataa gaacatgacc ctgaagtttc aatgcaggtg gcaatttgga
     8281 atttagttaa caagttggca agttggcaag ttagcaagtt ggcaagttgg gttgactggc
     8341 cgcagacccg ggtctttccg gtcatgcgct gattcttgtc catcgcataa actaaatgta
     8401 cctttgcggc tctttaataa ctataatgga caggaataat attgttggtt ttatcttgat
     8461 ttttgtcgtt ttgcttttgt ggtcctattt cagcaccagg caacatcaac aggaggcgat
     8521 tcagaccaga atccaggact ccatcacccg ggtccagatc attcaggaca gccttcggaa
     8581 tcaaatccgt gcttccggta cgggaactga taccctggcc atggccgcgc ctgcaggacc
     8641 tcaggccggg atggccggag tgctcgtgga cagcacggcc aaagccagcg attataccct
     8701 cgaaaatgaa gtgctgaagc tgactttaaa cagcctgggc ggccagatca ccaaggctga
     8761 attgaaagag tataaaaaaa tggtcgtggt ctccaaaggg ccggaccaga aagctccact
     8821 ctacctgctc catcacccca gcaatctatt gaaccttact cttccactca gggacaggcc
     8881 gctcgaaacc aaaaacctga tcttctcagt acccggatcg gatccctcgt ccgtgacttt
     8941 cagggcggtc accccggatg ggaaacaatt ggatttggaa taccggctgc acccgggcag
     9001 ttacctcgtt gactttaaaa tcagcctgaa tggatttgac caggacctgg ccgaaaaaga
     9061 aatcgtgcta gattggcaga attacataga gcccctggaa agaggcatcg cttatgaaaa
     9121 aacactgacc tcgcttcatt acaagccctt ggcggacgat aaagaaaggc tgaaggcggg
     9181 cggggccgaa aaagaatctt tcgaaggtcc ggtaagctgg atctccaacg cccagcaatt
     9241 cttcaatacc agcctacttg ccgacgaacc cttccggaac ctcaccgtcg agacgactcc
     9301 gggcccggag ggttccgaat acctcgcccg attcaattcc cgggtatcca tacccttcca
     9361 ggtctccggc gcccagggct taaaatggta tatcggaccc aatgaattcg gcctgctgaa
     9421 agcccagggc aatggtatgg aagacatcat ttcctttggg gcaagcatcc tgggaagcat
     9481 taacaaatgg gtgatccggc ccgtgtttaa tttcctttcc ggttttattg gcagcaaggg
     9541 cattgtcatc ctggttttga cctttatcgt aaaacttatg ctttttcccc tgagctataa
     9601 aatgctgcat tcccaggcaa aaatgggggc cttaaaaccc gagttgaacc agctgaggga
     9661 aaagttcaaa ggggaccagc aggcgatgca gatggaatca atgaaattat acagggaatt
     9721 tggagtgaac cccctgggcg gttgccttcc catgttgtta caaatgccca tctggctggc
     9781 tctctaccag tttttcccgg catccatcga attccgccaa gcttccttcc tttgggcgga
     9841 tgacctttct tcttacgatg tgtttgccaa attgccggta agtattccgt ttttcggagc
     9901 ccacatcagc ctgtttaccc tgatttgggt ggtctccacc ttgattttta cttattacag
     9961 caccaaagac caggattttt ccatgaatcc ggcgatgaaa tacatgcagt acatcatgcc
    10021 cgtttttttc ctcttcttct tcaacaattc tgcctccggc ctcacggcgt acatggcctt
    10081 cagtaatgtg ctcaatatcg gtcagaccct cgggggtaag gcgcttttat tccctgaaac
    10141 caaaatgaaa gaggaactcc acgccgcccg gcataaaccg aaaaagaaag gcggattcca
    10201 ggaaagactg gaaaccatga tgaaagaaca gcagaaaaaa atggatgaaa gacaaaaaca
    10261 ggtgaaaaaa tgaaatatgc ccccccggtc tttttcttgg ctctcctttt gttgctggct
    10321 tgcgcttcta ccgcgagatt aaagcctgcg tcgccgggtt cggcgtctcc tccaccccaa
    10381 gcagccattc cggaaaataa gccagcggaa aatgaagaga acaagccccc ggcggccgtg
    10441 atgattcctg aaagcaccaa atccagggat accattcccc cgcctgcttt taaaattgat
    10501 accctgttca cggtatccta tggcccctgc cagggtaaat gtccggtata ccggttgagc
    10561 gtgaccaatg acgggatggt gttttggaac ggctttcagc atgtggagcg gacagggcct
    10621 catattaccc gtttgaatga ggagcaaagg ggaaaactgg aagcagccat tggcagggtc
    10681 tttcattcga gcctgcaggc cagttatccg ggcaaggatg aatttattcc aaccttccca
    10741 atgaagacct ataccctgaa gctgaacaac cagatgaaaa ccatcgaagt caaccacagc
    10801 cctccccgcc aactgataga tttcgagagg caattgtttt cctggctgga aaaagccgac
    10861 tggcgaccgg taccggcccg gtgaaatcgg gaaagttttt tttgaaatct gcctacattg
    10921 gaacaggatg aaggaagtgg tagtcattgg agccggcagt tttggcattg ccgtttcaaa
    10981 attgcttgcc tataatgtcc gggtccgggt atttaccagg aaccccgaaa ccctgcataa
    11041 gatcaatgtg gaacaccaga tgttcaacgt ggccctttct gagcggatcg tagccaccca
    11101 gtccatggaa gaggcgatcc ggtctgccag ggttattttt cccattgtac catccgacag
    11161 ttttaatgcc atgatccagg aggcggcgcc cttcctcagt ccggcgcatg tgctcattca
    11221 tggtaccaag ggatttgtcg tgaaagagtt cggagaggaa gacctgggta aaagcgaact
    11281 gcggaaagag aaagtactca cgatcagtga attgatccgc cagcaaacca gtgtggtcag
    11341 gatcggatgt ttgagcggcc ccaaccttgc caaggaaatc ctggatggcc agccgacggc
    11401 cacggtgatc gcgagtgaat ttgacgaagt ggtccggata ggaaccgaat tgctttccag
    11461 taacaagttt tttgtttttg gaagcaatga cctcaaaggg gcccagatcg ccggggcgct
    11521 gaaaaacatc atcgccattg gcagcggtat acttgacggt ctggggctgg gcaagaatat
    11581 ccaggcgatc ctcatcaccc ggggcctggg ggagatcatc cattttggcc agagaatggg
    11641 ggccggcagc agtgcttttt tgggtaccgc cgggataggt gacctgatcg ccaccgcaac
    11701 cagcaccgac agccgcaatt accagttcgg caaacgcctg ggcaaggggg agaaaattac
    11761 gacggtcctg aacacttccg aggaattggc tgaaggagcc cggaccctga agatcgtcta
    11821 tcaccttctc aaatccttcc gcatgaggtc tcccatcatt gagatccttt accggaccgt
    11881 ctatgaggat tataacatcc aggaagggat caatttcctg atgcgttacc cggtcaaaaa
    11941 agatgtggat tttatcgagg aggattgacg taattttgtc cacagcatga aggtgatttt
    12001 ccgaagcctt gtgtttttaa tcctggcggg ggtccttgca tttccctttg tcaggatcat
    12061 gtatattcac ctgagggtgg tctcccaccg gaaaatcatg cagggtgagg aaatgggcga
    12121 ccatcccgtg gaaaccctga atctgaccgt tgccgaacta aacaggtttc gcctggatga
    12181 acgggaactc atttatgaag gaaaaaggta tgatatccgg acagaaaaac gccgggggga
    12241 tagcgtacgg cttttcgccg ttcacgactg gtatgaacag tatcttctcc aggaaatcat
    12301 cgatcttcat gaaaagggaa aacaaccaga ccagcaggta gcccggatgc tgacacctga
    12361 ctggatcatt gtgaaaatcc tccggctgaa tggtcccaac aaaccggctc accccctgcc
    12421 cttcccggaa tacctaatcc gctggatgca tactccctgg cttacctggt ttccacctcc
    12481 ggatgggaat atttaaaccc aaaccatgca atagattttc tattacaaac cggaatgcct
    12541 gcaaatactg gcacttcccg gccgaagaag ttgatttatt attaaggtcg gcctcccatt
    12601 tttatcctca ccataggagt gcctatgttt gcggtaaaaa tagtccggga gcgccagtct
    12661 ttattggcct tccggctttc ataacgaaac tctattgaat gatttgggtt aaacccttga
    12721 caagcaatag aatttctata cctgcggaag ttccctttaa ttccagaacc tccggacttg
    12781 atgggcgggc ctcccccctt tcttacaaaa cctggttttg atctattccg aaaaatggat
    12841 agaccggatt ttaccgggga taaagtaaaa acattcggga ttttgcccat tctgatcgga
    12901 ttttcgccct gcaggatgaa aagctatgca ttgatttggg ttaacactga cgttttcggt
    12961 accctgccgg tatctggaat tatttataaa aaattaaacc aaatatcata tgaaaatacg
    13021 atggctggtc ctgggcctgg tactgaccac ccgggctggc agccaggata cgattgccta
    13081 tacccaggaa accgatgagt tggtgatcag cgccacacgc agcgccatgt ctttccgcca
    13141 gattgctcag accgtaaaat cgatcactgc gaaagatctg gaatattcca tgccccagac
    13201 catggccgat gccctgcaaa aaacaggtgg aatcaccgtt caaaaaagcc aacaaggagg
    13261 gggaagcccc atccttcggg gattcgaggc aaaccggatc ctccttgtag tggatggtgt
    13321 aaggctaaac aacctgattt tccgaggtgg ccatttgcag aatatcatca ccatcgatcc
    13381 gaacatgctc gaaaaaacgg aaattctgtt cgggccggct tcagtggctt acggttccga
    13441 cgcacttggc ggggtaatcc atgttctttc ccgtaaacct gccctgggct tgccgaaagc
    13501 ttttcaatcc tccgtggcag cccgctacag tacttcaaac caggagaaaa tgatccatta
    13561 cgaaggggcc tggagcggta agcgctgggc agcccttctc tcgggaacct attctgattt
    13621 tggagacctc aacgggggaa ggaggaaaaa tggtttctac gaccaccgct acggataccg
    13681 ctatcattac ttcaattttg aaaacggaaa agatgttttg aagaccaatg acaagccctg
    13741 gaagcaggtg ggttcaggct actggcagac cgattggctt ggaaagatcc ggtattcaag
    13801 cggaaaaagg acggaccata tcctgaacct tcagtattca acctcttctg acatccccag
    13861 gtatgaccgg ttgacggatc cggataaaat gaccgtgctt cggaacggcg actggtatta
    13921 cggacctcaa aaacgcttgc tgatgtccta ttcgggggaa ttcaagaccg gtttccccct
    13981 gcggataaac ctgaattacc aacaggtgga agaaagcaga cacaaccgtc gggccggcaa
    14041 tcccaacctg caaagccggt ttgaaaaggt ggaggtgctg ggcttcgatc tgctgcatca
    14101 atccggaaac cgggtccacc aatggatctt cggactggac ggtcaatacg aaagccttca
    14161 ttcttcagct ttcctggaaa acaagacaag tggtacccgt tcaccgctcg acacgcgtta
    14221 ccctgccggt agaaacagga tgatgcgatc gggcctttat ggcatgttca ctcaagtcat
    14281 ttcggaaagg ctggctttac aggagggttt acgtttcggt ttcacgtccc tgaattcaag
    14341 gtttggaacg aaggattttt ttgatttccc atttgaccgg gttcgccaga cccatttcac
    14401 ttattcagga aattttggac tgagttacaa cccgggacca gataccaaat ggggagcagt
    14461 tctggctagc ggattcaggg ttcccaatgt agatgacctg gccaaggtat ttgaatctac
    14521 cccaggagtt ctgatcgtgc ccaatgatgc actccgtccg gaaaagagcc tgggcctgga
    14581 gtttaattat acgcattggg gaaaagaccg gcgttcacag atccagatca gttcctacct
    14641 gacttatttg tttgacgcta ttatcacctc ccctttccgt tacctgggtc aagacagctt
    14701 atggtataat ggggtcatga gcagggtttt tgccaatcag aatcaaaaca aagcccggat
    14761 tgccggcttg tccctggaag gccgaactcc ggtgacagac cgtatcctgg tccagttggt
    14821 caccgccatg acccggggcc gcattttgcg taaatcgggc aatacccctc tggaccatat
    14881 tcccccctta tccggtaagg tgggcttgtt ttacgaggaa aatcggttta acctggaatt
    14941 caacagctta ttcaatggct ggaagcggct ggatcaatac ctcctcaatt cggaagacaa
    15001 tgaagcttat gctaccccgg atggcatgcc agcctggctg accataaata tttatgccgg
    15061 gtggcaggtt tcggaatcca ccaggctccg gctcagcctt gaaaacttgc tggatacctc
    15121 ttaccgggtc tttgccagcg ggatcaatgc ctcgggcagg aatttgagtg ttatgattaa
    15181 gacgggattt taatgaagta aagggtggag gggagattcg tcccctcttc cctattcctt
    15241 cccacctcgc acatcatctc aacatattac cactatttta aatatattgc aggtttaacc
    15301 cgatcaaaca acctatgcaa aaaatagtat gtccggcctg taatcacgct tttcacgcgg
    15361 aagaagtcat tagccgccaa ctgacccggg accttgaaaa aaaattcagg gagaaggaag
    15421 aatccctgct caccgacttt cagaagaaaa tgaacctgct ggaaggccgg gagcaggagc
    15481 tggaagccaa aatccggaag gaaaacacct tatttgcaga acgcatagaa aaagaaaaga
    15541 taaaaatcca ggaacaaatc cggatccagc aggaacggga tttcagaatc aaactcgaca
    15601 ccctggaaaa agaacgaaat gaacaggccg cccggctgga agccctgacc caggccgcgg
    15661 tcgagaatga acaactgaaa cggaagctgg ggactttaaa atcggacctc gaactccaga
    15721 tgcaaatcaa aatgacggaa gagctcggca aggaccgtga gcaaatacgc agggaggaat
    15781 ccagaaaagc ggaattgcgc caaagggaac ttgaaaaaca actggatgat cagaaaaaac
    15841 tcatcgaaga cctgcagcgg aagacccgcc agggctccag ccaggcggtc ggtgaagccc
    15901 aggaactggc cattgaggat tatctcaggg aacagtttcc cctggacgag attgaagaag
    15961 tgcgcaaggg ggtccggggg gccgattgca tccaaagagt ccatacccgg cttattccca
    16021 acgcgggaac gatttattat gaaagcaaaa ggaccaagga tttccaggcc ggctggattg
    16081 aaaaattaaa ggaagatatg aggctggtgg gcgcagacct gggcattctg gtgacccagg
    16141 ccatgcccaa ggatatgccg cacatgggtg agcgccaggg gatttggatc tgcagctatg
    16201 aagactttaa acccctggcg ggcatcctgc gtcacggttt gatcgaagtg gctaccgccc
    16261 ttcgttccca ggagaacaaa tccgataaac tccacctgct ctatacctac ctcaccggca
    16321 atgatttcaa gatgcagata gaagccatcg tggaagggtt taaacaaatc caggatgatt
    16381 tgcagaaaga aaaaattgcc atgcaccggc tctgggcgca aagggagaaa atcatcgaca
    16441 aggtactgct caatacctcc cagttttacg gcagtgtcag gggtattgcc gggagtgcga
    16501 tcccggtgat tagcgtactg gacctgcccg gcgaaccgga gtccctttga tctgaaaaaa
    16561 gccgaacttg gtaagggctt ttaaggcact cagcgataag atgaaagaat tcctgttaaa
    16621 tccccgaatt ttacacgcat taaacacaag gcatgcttaa ccaactcaat gaattgtttc
    16681 aatcggccaa cctggacggg aaaagccttg aatttctcaa taatgccatc aaagcccagg
    16741 ccctgcaggg ttttgactac ctcaaattca aacaatcgat caaggcgctg caggacatga
    16801 gcatgagcga ggaaatggcc atccggtccg ccttttccac cgcttccacc atggggatca
    16861 ccaaacagaa gatcctggaa aacatccagc attaccaggg tgtgttgaac aaagagaagt
    16921 ctgaattcga ttctgccctg caaaaacaga ccaatacccg catcaaccag aaacaaaacg
    16981 aaacgtccta cctgcaggaa aaaatcaaag cgcatcaggc gcagatcctc gaattggaaa
    17041 aacagatcaa ggaattccag tttaaaatcg acaatgccga caaggaaatc gaagaggccc
    17101 gccaaaagat cctggacacc aaagagaggt ttgaagaaac ctatacgcac tacatggacg
    17161 tgctccggag cgacatagaa atattcaaca aatatttata attttaaact taaatcattg
    17221 atatcatggc agaaacaccg ttcaacccgg ctcccgctaa acccaagtcc ttctggaaaa
    17281 ggccggaagg agtcacgggc accattgtcc tgcttggttt gctggcaggc ggagggttcc
    17341 tgttgtacaa ggccctgcct accctcattg ccctggccag caatatgctg accctggtcc
    17401 tgatgctcct ggcgctgggc gccatcatct acatggtcct tgacccgaag atgagggccc
    17461 tggtggggta tgcctataaa agcatcatgc gctgggtcac cggcctcttc atcaccattg
    17521 accccatcgg cattctcaaa aattatgtgg aagacctgga ggacaattta tccaaaatga
    17581 gcacccagat cggcaatctg aaaggccaga tccgcaagct caccaccatg gtcgaagaaa
    17641 ataccaagga gatccagcag aatatggtga tcgcctccaa agccaaagaa ctgggccagg
    17701 aaaaccagat gatcctcgcc agccggaaag cggcccgcct taaggaagta aatgtgaaat
    17761 acgacgagct ccggggtaaa atggatatac tttaccgggt gctcacaaaa atgtaccaga
    17821 attcagaggt cctcctggaa gatacccgcg accaggtcaa aatcaaggaa gaggaaagaa
    17881 aagccatccg ggcttcccat ggagccatgc gttcggccat ggcggtattg tccggcgata
    17941 aagacaaacg ggccatgttt gacctggccc ttgaaaacat agccgatgat gtggccgcca
    18001 aagtgggtga gatggaacgt ttcatggaat tgtcttcaaa cttcatgaac agcgtggacc
    18061 tgcaaaacgg cgtatttgaa gaagaagggg cccgaatgct ggaacaatgg gagaaagaaa
    18121 gccacctgct cctgatgagc ggcaaggaaa aagccaacaa cgattacctc aacttgggag
    18181 agcctcagaa ggccacccgc agcagcggag gttatgaggg catgtttgac gccaaataat
    18241 tttccggttt ttcgtgtacc aggggaccgg gtccctcaat gaaataccat ccgtcgatca
    18301 gttccccagt ttgtttattt catcaaacaa ggctttgaag gcatccacat aattgccacc
    18361 cctccgggtg ttgaccggtt gtcttccgta aaattgttct ataaaattat ctccgccggt
    18421 ccaggtggta tggaggacgc ccaggtaatt gtttttcacc gccggagtgg cgaatttcct
    18481 ttggttgacc atgatcttca attgttccag ggtcacatcg ggaaagcgcc agggacaggt
    18541 aagcacccgg aaacctttta aggcaaacag gatggccgtc ggatccggcc gttcataatg
    18601 ccagtcacat atcaaaacgt ctttcgggat cagatcaatc gcacgggagg tgttgttgta
    18661 acttccctcc cagaaaccga ccccggtggc ctttccgtcg atcagccggt ccccccagat
    18721 ccagagttcc ttgttgtttt tggccaggtg gttccggatt gcggtcactt cgccggcaaa
    18781 caattccgcc ttgtcccgtc ccgaacaacg gggacatttt tcttccccga gaaaaaagac
    18841 ttcatccatc cctgcgtgaa aggcatcggc ttcaaagact tcaaccagtt cgtccatggc
    18901 ctcgaagacc acctggtgaa cctccgggtg cagcgggcaa tagctgaaac aatacaatcc
    18961 ccattcattg ggccaggtca ccgtactcga atcctgcctc gaaggaatcc agggggtttc
    19021 attgaactgg ggataagcct tcagcaaggg tcctacctta tttgtggacg attggtggcc
    19081 gagcaggttg atctggggga tgatccggat cccgccggct ttacaggcat tgacgatttt
    19141 tttaacctgc atctcggaaa gggcgttgtc cgccaccagt tcaggatggg atttgaattg
    19201 ataccggtac ccgatcagga ataccagggt attgacttta cggggcacca gttcggaccg
    19261 gatgaattcc gtaaactttt ccacgccatc gggacttggg gccgacaggc agattccccg
    19321 gaccggaagg attgaatcca gcgtaccctg tgccggcgat tcggcaatcg cggagaagag
    19381 gaggatcagg aaaaaggtta ttttgttcat agccggcaac ttatttatct aatttattaa
    19441 tttcttcaaa caggaccttg aaggcttcga cgtaattgcc tcctctgccc gtgttgactt
    19501 cttttttgcc gtaaaactgg tcgatgaatt cttccgcccc cgtccaggtg gtatgcagca
    19561 tgccctggta attgtttttc acggtccggg aagcagattt ccggctgtcc accatgattt
    19621 tcagttgttc aaggctgacg tcaggaaaac gccacggaca ggtgatgacg cgaaacccct
    19681 ttaaggcaaa caggatcgcc gtgggatcgg gtctttcata atgccagtcg caaatcacta
    19741 cgtctttggg gataaggtct atggcccggt gcgtgttgtt gtaactggct tcccaatagc
    19801 cgatgcctgt ggttttcccg tcgatcaggc ggtctcccca gatccagagt tcccggtttt
    19861 tcgatgccag gtggttcctg agcctggtca cctccccggc aaacaattcg gcatggtcca
    19921 gtccggcaca gcggggacat ttttcatcgc cgagataaaa cacttcgtcc atcccggcat
    19981 ggaaggcgtc cgcttcatat acatccatca attcatcgat gactttaaac accactttat
    20041 gaacgtccgg gtgcagggga caataactga ggcagtataa accccattca ttcggccatt
    20101 tcaggtcccc tgagccctct ttcatgggta cccagggcgt ttcattaaac tggggataag
    20161 cctttaacaa ctgacccgtt tgctgaaacc aggactggtg gccaagcata ttgatctgcg
    20221 gaatgatccg gatccctccc gccttacagg cctttactat tttttttacc tcttttttgg
    20281 ataaggcgtt ttccgccacc agttccggat gcgatttgta tttataattg taatccacga
    20341 ggaggaccag ggtattcacc cttcgggtca ccaattcatc cttgataaat ttcacaaaac
    20401 ggtctacccc ttccggtttg ggcaccgaaa ggcatatgcc gcggacggga agaacggaat
    20461 ccagcaccga ctgggaagtg gcggtaccgg aaaggcttat tagaaggaat aaggagacgg
    20521 ttcgaatgaa aaggtttgcc ataggacggg tcgtttcagg tttcggtttt ccgaagatat
    20581 aaaaagcgaa aataggatca aaaccgttat tttagtaaag caagcgcttc aatatgaaaa
    20641 ccatcttctt cctgttggcc ggcctgtttc ctgtttttct tgtttcccag aacgaacacc
    20701 cggttgaata caagatcgta agtatttttt tcagcggggg cagttattat atatccgagg
    20761 aagagaagat gaaggtccgg gaattcctgg ccaatgaaat cctgggcaat tacgagatcc
    20821 acatccacag ccatacggac aatatcggcg gagtgcggtt taacgaatgg ttatccaaaa
    20881 tgcggagcga atcgaccctg gactttttga ttgaagacgg ggtgcccacc aataaagtat
    20941 tcatcaagga tcacggcctt tttaaccccg agttcgacaa ctccacctgg gacggcaggt
    21001 tgaaaaaccg gcgggtggac gtggtcttgt ggcccctgcc tttctgatcc aggcgattca
    21061 gcccaatacg atggttttgt tgccggagac aaataccttg tcctccacga tctttttcag
    21121 ggcttccgcc agcacggctt tttctatttc atgcccggct tcgatcatat ccctgagttc
    21181 gtgttcgtga tcgatttcga tggtcttttg cacgacgatg ggcccttcgt ccaggtcgtc
    21241 gctgaccaga tgagccgtgg ctccgatgat tttcactccc cgctcaaaag cccgtttata
    21301 tgggtttgcg cctttgaagg cgggcaaaaa agaatggtgg atattcagga tgcggttttg
    21361 ataccggctc acaaaatgcg gggagaggat cctcatgaat tttgccagga ccaggtagtc
    21421 cggatcaaac cggtccaggg tttcgatcat ttgcttttca aagacggtcc tatcctcctc
    21481 atgcgggata tgaaaaaagg gaatgcccat cctttcggta aatggcccca ggtccccatg
    21541 gtttccgatc accgccagga tcgaagcatg caactcatca aaatgatgcc tgaccaggag
    21601 gtcgctgagg caatgatgtt cccgggtgac gaagagaacc agctttttct tcctgcgcgg
    21661 gatgatggtg acctcggccc cttcgggaag gacgttccgg aagcggctgg acaataaatc
    21721 ggcattcagt gtgccggtca gctcaagacg tgcaaaaaaa gtattggtgg attgttccac
    21781 aaattctttc atcaccagca cattgtgatt gtgttgaaaa agaatacccg atatcctggc
    21841 gatcagcccc ttttgatcca tgcactggat gcggaccaca tggttcttgt ccataggcgg
    21901 taaccggtat tgatgggaat tatttgacca gctttttctc caggtcctcc agggataccc
    21961 ctttggtctc aggcataagt ttccatacaa aaagaagttg gagcaccatc atgagggcga
    22021 aaaacaggaa gatggtgccg cccccgtatt tgctgttgaa atggggaaat aaaccggcga
    22081 tcagcgccgc aaaaagccaa tgcgtgctcg atccgaggga agccccccag gacctgaccc
    22141 ggttggggaa aatttcagag ataaaaaccc agatgactgc cccctggccg atggcgtggg
    22201 aagcaataaa aagaaacaaa agccagggca ccatggtccc gccggttccg cggaaaaaag
    22261 caatggatac cagggaaagg gagaggatat atccgaaact gcctataaac atcagaaaac
    22321 gccggccgta gcggtcgatc agactgagtc cgagcatggt gaaaataagg ttcaccaccc
    22381 caatacctac ggtggataag agggccgctg actttccaag tccggtttct tcgaggatcc
    22441 gcggggcgta atagatgatg gcattgatcc ctgaccattg attaaaaaaa gcaaacagga
    22501 atgcaagcag gatggggaaa ttgaatttac cagaaacaaa atgcgccagg ctggtttcat
    22561 tttccgggtg cgggctctgg atggctgcga gggaagcctc cgccgtaccg ggatcgatcg
    22621 tcttcaggat ttgttttgct tcctccgtcc tggatcttct ggtaataagc catctggggc
    22681 tttcaggtac aaacaggaga ctgatgataa aaattgctga tggaatggcc atgattccaa
    22741 gcattagcct ccaggaatcc cccgtcccgg cgccaatcag aaaattggag aaataggcca
    22801 tcaggatccc aagcaccagg ttgaattgaa acaaggcggt cagctggcct cttttatgct
    22861 ggggggcgat ctccgatata tacatggggg cgaccacgga cgaagcgccc accccgaggc
    22921 ctccaatgaa ccggaaagcc atcagcatct ctacgttcaa ggccatagcc gagcctatcg
    22981 aacagatcag atataaaatc ccgataacaa aaagggtgtt tttccggccc catcgttctg
    23041 ccggtatccc cccggtaagc gcgcctatga tggtcccgta caatcccatg gccaccatct
    23101 ggcccaccat gacatcactg aggtcccata atttctggat ggcttgttcg cctcccgaaa
    23161 ttaccgcggt atcgaaacca aataaaaagc cgcccaggga taccgcaaga gaccagaagg
    23221 tcacatttcc caccgtttta atcatatgca tgtttgtttg aaccaaatgt aacaaattga
    23281 ccatctttcc ggtacaatgt tttattttcc ttccatgaaa acaagcggaa tcaaaagctt
    23341 cctgtccggg gtgctccagg gcagcctggt attcctgatt catggcattc tcagagccca
    23401 gacgctgcaa ttgcatccgg tcaatcctca ttatttcaat tataaaaaca aagccatcgt
    23461 gctggtcggg tccggggagc attacggggc ccttatgaat acggcctttg acctggaaac
    23521 ctatttcgcc acccttaaga aggacgggct caaccatacc cgggtgttta tgggtgcata
    23581 ttttgaaaaa ccgggcgctt ttgggattga aaagaatacc ctggctccaa agcctgaaca
    23641 aatgctgctg ccctgggcct tccaaggcgg ctcatacgac ctgaccctct ggaacccggc
    23701 ttatttccaa aggctgcacc aactgatggc cctcgcgggg gaagcgggcg tcatcgtgga
    23761 agtgacttta ttttcatcct attatggggc gggttgggat tatcaccctt ttcatgccaa
    23821 aaacaatatc cacggcctgg atgaaacact ggattaccgg aaagcccata ctccggacaa
    23881 cggcaaacta ttgcaatacc aggaaactta tgtccggaaa ctggtcgcgg aattgaatgc
    23941 atacgatcat ttttattttg aaattcaaaa cgaaccctgg gcggatatcc gggataccct
    24001 gatcgtctgg aaccagaatt tcaggcagga agaactcaag gttgcaggaa atttctggaa
    24061 aaacaccatt gaaatacctt ccggactttc cctggactgg caccggacgg tgtccggatg
    24121 gatccgggat gaggaaaaaa accggcctaa aaaacacctc atttcccata acatcgccaa
    24181 tttcaaactt ccggtacccg tcacggatga ccgcatttcg atttacaatt ttcactatgc
    24241 ctttcccgaa tcggtggatt taaattataa actgaataaa gccattggtt tcaatgaaac
    24301 cggttttgcg gggaggctgg actccatcta ccgcaggcag gcctggcgtt ttatgatgag
    24361 cgggggcgcg ctgttcagcc acctggacta ttctttttct acggaccatc cggatggaac
    24421 ggataccctg cagaaagccc cgggcggggg cagcccggcc cttcgtaaac aattgaggtt
    24481 tctcaggacc tttgtggaag gcttgaacct ggcgggcctc aggccggccc ctgattttat
    24541 atatcacgtc gaaggagcct tccgttgggg tatgcaggac gggcgccatt atgccctcta
    24601 cctggaggcc ctctccccca accccgcgaa agtgcagtta ttgcttcgcc cgggaaatta
    24661 cctggctgaa tggtatgaag taaacaccgg ccggaaaatt cacagcgaac cggtcaaggc
    24721 tgttaaaaaa tggtacgagg gcaaggcacc ggccgggatg gccgatctgg tcctcctgtt
    24781 gaaaaagacc cattgaatga tcagcggatt cacttttctc cggaatgcgg gagccctcta
    24841 ttatccctat gttgaaagca tcctgagcat cctgccgctg gtcgatgagt tcattgtagt
    24901 gctgggggaa ggggacccgg gcgaccgcac ccgtgagaag ctggatgaac tgaggtcgcc
    24961 caagctgaaa atcctggatt cggtttggga taccggccgg taccggggag ggtccatcta
    25021 tgcccagcaa acggacctgg ccaaagcagc ctgccgcgga gattggttgt tctatctgca
    25081 gggcgatgag gtgatccatg aagaagatca tgccttgatc cggcaagctt gtgaagctta
    25141 tatccaggat cctcgcgtcg aagggtttgt ttttgatttt ttacactttt acggggatta
    25201 ccggcattac ttccgcgacc attgctggta caaaaaagaa atcaggatca tccggaacct
    25261 ccccggaatc cactcctggc gtgatgccca atcgtttcgc tatatccccg attttaagga
    25321 tcctgattat ttcaggacgg agggcactcg taaactcaat tgcattgccc tttccgcccg
    25381 ggtttttcac tacggttggg tcaggccccc cgacaggatg gccctgaaga atgccgaagc
    25441 agggaaaaac taccagaagc ccgggtacga aaaatttcaa ggtcggtttg attacgggag
    25501 gttggaccgg gcccgggttt atgatggaaa gcatccttcg gtcatgaacg attggatgac
    25561 ccagttgaat tggcaggacc ttctgcgttt ctccgggcct gcggctttaa acaggcccct
    25621 gatgaaacat gagaaactca ggtaccgaat ccttcattct atcgaagaaa ggatactggg
    25681 aggccgtgtg atcggcggtt tcaggaatta ccacctgctg caggcggaac aataaagcct
    25741 gtctttgacc atccggttta aaattggact tcaagggatt tgacctcgcc attccttttt
    25801 atttctacat ggacccggtc ccccttctga aaggacccca gggcctccat ataggcatag
    25861 atatccttga tggctttctc acccattttg acaatgatgt cccctttctg cagccctgcc
    25921 agcgcggcgg gccttccttc ggtcaccccg tcgacgcgca tgcccccttc ctggtacagg
    25981 taatcgggca tgacccccat gcttactttg aaggccacgg cgcgtttgcc ggattcatcc
    26041 tttgttttgg taaaggccag cttgcctttg gaatccaggt cttcgatcag gcctatgatc
    26101 aggtctgaga tttgcaggat cccctcgtaa tttaccttgt gggcgtcatc cgaaggtttg
    26161 tgatagtcct ggtgctgacc ggtaaaaaaa tgtaaaacgg ggatatcctt gagataaaaa
    26221 gaggtgtggt cggaggggcc ttgcccggac tcggtggcgc ttattttcat tccgttggcc
    26281 atggcctggt tttcaaaacg gggtttccag gacggggagg tccctacccc gttgatcgcc
    26341 aggatctttt catggttgag tctgccgatc atatcgagat tgatcatgta attgactttg
    26401 ccggcctgaa acaaggtgga gtccttggta taggcattgg acccgaagag gcccttctct
    26461 tcgccggaaa aagcaataaa aagatagttg ttgtttttgg ccttcaggtt gtttttaagt
    26521 ttcgccgcaa tggccagtaa acccgccaca ccggatgcat tgtcgtcggc cccgttatgg
    26581 atggacgtat cgtttgcagc cagagaaccg gaccccgtac cgccaaagcc caggtgatca
    26641 taatgcgcgc cgatgacaat ggtattcggg gcgccgtgat ccagcaaagc cagcacgttg
    26701 ttgcctgttt tgaccgggcc cagggacctt ttggccgtat cgtggggatt gagggtttcc
    26761 tggaaattaa acggataaag ccatccgccc ttcaagcctg ccggcttcag cccaagcgat
    26821 tcaaaccggt gcctgatgta ttccgccgcc agcttttccc cttccgtggc cgtttcccgc
    26881 ccggccagat aatccgagga aaggtaaacg acatcgattt tcgcttgcag cagatcctgc
    26941 ggattctggg cagtcatcca gccgtttgtg cccatgagga ataaaaccag gaagtgtatt
    27001 tttttcatgg cgcaaaaata ccggatttcg gcatggaggc aagccggatg caggaccggc
    27061 agggcttgtc cctgatcagg gtcatggaga ccggtgacca tggtatcaaa aggtcatgga
    27121 cgcggagact aacttgccat aataaattaa aggattatga aaaggaattt atcaaacctg
    27181 gaccggtgga taagaatggg actggccttt ctaatcgctg tgctttattt taccaatgcc
    27241 atcagcggca cgacggccat catcctggcc ctcctgggca ttgtattcgt tttgaccagc
    27301 ctggtaaact ggtgcccact ttatcatgtt ttgggtattt ccacctaccc caaaaaagga
    27361 caacaagtct caacgggcaa ccctaagccg gtatagtccg gtggaatagg ctccccatac
    27421 cccgaggccc cctttaatat tagtatcaat ggtggtatag gatgaaaagg ggccttgatt
    27481 gcctttgttg aattcaaagg tattccagaa attaaaatgg gcttcatcaa tggaagtcca
    27541 tttgagggta atggaatcgc cggtccggaa aagaccgaaa gtggactggt cgaattcctc
    27601 tccttcctgt cggggacgta tcaggcggaa atcaaaagtc tttccgtcga tcaggatatc
    27661 atcgttgacc gaactaaaag gggtttcaaa ggggccttca ttgactttgc agaaataacg
    27721 gtaaaagttt ttttgacctg gcgggtcctg aattctggcc atgagttgga gcagggtatc
    27781 caccggcttg cccggcgcct ccctccacca aagagaatcc aggggtacgt aagcgggcac
    27841 agtggtagag gcttcaatcc gttgatcacc ggctacgatg ttcagatggt aagattttcc
    27901 ggcttcgggt ttgatggccg cgttgaggtc gatgtataca cagaagttaa ccaatatact
    27961 gtcagggttc aatccaaggc gtgccgcggc cagtcggcga aggtccggag gaagttcatt
    28021 aaaacacaat tcgtccaggg ttgtggtttg ttggccatcg tttaccgtga ccacagcgtt
    28081 ccggacaaag agggcatcca gttccgccgg ggaaagttcg ctgaaaaagg gttttgaccg
    28141 ggtcaggatg acataggcgg gcagggcttc gggtccggct tcgataaacc cttccactac
    28201 gatttcaggt tccaggtcga tattcctggt tataaactca acttcgcagg ctggaaagaa
    28261 ggataccata aaaagcagga taaggatccc gacggaaaag agcctggctt cccggttctt
    28321 ggttttttga tcttggagat tgggggattc catcatgatt tccatttaaa attccaggtg
    28381 actgaaggga tgagcgggaa aagcgtaacc tggtatgccg tggctttcgc cctgccggca
    28441 gaaaggtcgg agtccaggtc atagttgata aaaaacggat tcttgcggtt atataaatta
    28501 taaacagaaa aagtccatga accggagaac gctttttttg aatcgggttt aggggtgtag
    28561 tttgctgaaa aatctgcgcg gtgataatcc tggtagcggg cgctgttccg cggcccgtaa
    28621 cgggtcacca ggtcctgatc aatgaaatag acactgcgca ggggggtaaa gggcctgccc
    28681 gtcccgtaaa caaaattagc cgacaggctc cacttgcgtg agacctgata gctggctacc
    28741 atttcaaggt catgcggctt atcgaaagaa gccgggaatt tccggccttg ttcaatgtcc
    28801 cggaaccagc gccaactccg ggtaaaggtg tacccgagcc agccggtcag cttgccacgg
    28861 gatttttgca caaacagttc caggccatac gcttgacctt ttccaaagac aaaagcctct
    28921 tcaatttccg tactttgatt gctggtatac gtttcgtggt aatcaatctg gttgcggaga
    28981 cttttataaa aggtttccgc cgaaaggttc caggtcccgt ccttcagggt cttgtaccag
    29041 cctgccgacc actggttgcc ccgctgaggt ttcacataaa ggctgctcgg gacccagaca
    29101 tcggtgggca gggtgcttgc gctgttggaa accaggtgga tatattgttg gttctggctg
    29161 tagccgaatt taagggaagt ccttgggttt agcatccagg ttaaaaacag gcggggttcc
    29221 agtccgtcaa acgatttcac tgcttcaaaa tcatcataga ctttatcccc gtcccggaaa
    29281 ggaccgatct gggtaaagcg gctccaccgc aatcccaggt tggtggtcag tcgctcgctg
    29341 atccggattt catcctggaa aaagaccgcg gattcaaggc cgtacttggg tttgtaatca
    29401 ttggtaaaag tggtttcgcc gctggccccg ctaaggatgt tgggggtaaa ccggtgatgg
    29461 atcacattgg ccccaaagcg gataaaatgg ccgggtgcag ggtaataatc cgtgtccagt
    29521 ttgagctgaa aatcgcggac cccgttgttc aatcgaaagc tgaaaaggtc ttgttctccg
    29581 tcgagcctga aacggtagcc attgtaaacg gcggtggtat tcatgaacaa tttattgctg
    29641 accacgtggt tccatctcag ggtggccgta ttgtttccgt atggcatccg gagggcaaaa
    29701 tcccgttctc cagaccggta atccaggata tcccggccaa aatagccgct cagatagatg
    29761 cggtccttgt cggaaaaccg gtgattgacc tttgcgttga ggtcataaaa gtaatagttg
    29821 gtcccggcga aatcggtctt gcggataaaa ggctgggcaa ggtccagcag ataggtccgt
    29881 ctggcggaga cgatgaagga acttttgtcc tccaccaagg gcccctggac ggtgagtctg
    29941 gacgcgataa gtccaaggcc cccttccacc acgtaatccc tggcgttgcc ttctttcatc
    30001 tggatgtcca ccacactgga cagacggccg ccgtaatagg cgggcatccc ccccttgatc
    30061 agcgaggtgt gtttgatggc atcggaattg aacacactga agaaacccag caaatggccc
    30121 gaattataca ccggggcttc atccaacaga aggaggtttt gatccgctcc cccgccccgc
    30181 acataaaagc ctgcgcctcc ttcatccgcg ctgagtaccc ccggcagaag ttgcagggct
    30241 tttaaaacat cgacttctcc gaacagcgcg ggcatccgtt tgatctgatc ggtgggcaga
    30301 tcgaccctac ccatggccgt actctgcacc tgctccaggc ttttgttttc ttccaccacc
    30361 agttcttcga gctggatccc tggattaaac aggatgtcca gtttttgatc ctggctcagc
    30421 cggacggttt caatcctgtc ttcaaaaccc aggtaggaaa aaaccaccgc atattcgccg
    30481 gccggcaggg acagggaata aaaaccataa tggtttgaac tggtaccgat ctggggcctg
    30541 tcccggacat aaacattggc gccggtcagg gtttccccgc tcagcgagtc gcgcagatag
    30601 ccgctgaggg taaatgtttt ttgctgggcc aggaggtcca acgataaggt tagcagtaaa
    30661 aaataaagga atgccttttt catcagtaca ccgttgttca acaaaaatgg ggtggaaagg
    30721 atcaaatatt tccagtgctt tttattacat gagtgaaacg ggccttaagg caaagccggc
    30781 cgctttgagc atccttaaga tacccttgga gcccgggagg tgggccgcgc ccagggcaaa
    30841 gaaaacggga gggcctcctt tgaccaggat atttttgata aaagcggtca tctccctgtt
    30901 tctttggtca atcatcagcc ttctcatgcc gttgagttgt ctccggctta atttgtatag
    30961 atgggtaagg tcttcgtgag cataggcttc ttccagtctt tttagctggc ggaatgaagc
    31021 ggagagcgat ttcatctgcg ctttgacttg caggagctgg atccggaaag gaattttctg
    31081 atagatttca agctgccttt ccagggattc cactccgcct gtttcgaggc ctgcccgctt
    31141 tgccaaatgc cagagcctga tgtccagggg atctcccccg gaacgggaca ggtatttttc
    31201 atgaatcagg gccggaataa acaaaggggg taaatattgc agggcatcca ggtccaggcc
    31261 ctgccatttt tcaaggaagt gccttgcttt ggcataggat ggcggagcaa ggagggattt
    31321 caggcctttc cattccagtg gcggggcggg caatgaagtt tccaggagct ggcctatatc
    31381 cgtttctccg taaaaggcgc tgcaaccgag gatatagggt tccaccctcg gtacaaactg
    31441 ctgggatgcc gggctcgcgc tgtgcatggt gccaaaaaga taggaggccg gcgaggcagg
    31501 acccgcttca atgctccaga gcaaggcatt ccctgcgctc atgctcattc atattgcagg
    31561 ggcaatacca tggattcttt tgccgtggat aaggtaacca tacttttcag tcgctcgatc
    31621 tcttcgattt gggacagctt cttgaaaagg atactttcat aacttggaat atccttgcag
    31681 acaatcttaa tgagggcatc tgcctcgccg gtgatgatat acacttcggt gacttcgtcg
    31741 atgcgtctta ttttttccaa aaatcggtcc atcgtatccg gacttttcca gttcagggaa
    31801 accaggacga aggttttgac ctgaaaaccc atggccttac tgtccagttt ggcatggtag
    31861 gaggcaatga ttccggcctg ttcgagcttt tttacccgtt cgagcgtggg cgccggggat
    31921 aaaccgatta atttggacag atcgagattg gtaatcttgc tgttttcctg caggatttta
    31981 aggattcggt aatccagttt gtctaattta tagtcagtca tatcgggaat gttttaaggt
    32041 aaagggaaat aattctaaat caggggaata cacccaaatt ggaatagtcg ctggcgatct
    32101 tgtcaagagc gcagacaaag gccgcnnnnn nnnnncgtct tcacctttat ctgctatatc
    32161 aagtataatt gttcttgctt cttttgattt caaggatttg ctggagtgaa ctgaccatag
    32221 tttcttccag gccggaacgc accaggtcaa tttcatcggc cccttttacc agcaggtttt
    32281 tctcgcgttg gccgatgtat tttccggtga ggttttcgac catggaaacc aggttgccgt
    32341 aagcttgctg gtcgaatctt ttttcaatcc ttccgaatct catgtgggac aggtttttca
    32401 accattcaaa gtaggaaacc gtgacccctc cggcattgag gtacatgtcc gggatgatgg
    32461 ccacgccttt tttaagcaac aattcttccg catcggaact caccgggcca ttggccgctt
    32521 cggcgatcat tttagctttg atcttccgtg cattttgcat ggtcacctgg ttttccaggg
    32581 ccgcaggcac caggatgtcg cattccagtt cgagggccga catccggtca ggcaggtttt
    32641 tggccccttt gaaattaagg atggaaccgg tttccttgcg gtgcttcagc aattcggtca
    32701 cggaaatacc cttggggtcg taaatggacc cctcgtattc ggataccccg atcaccttga
    32761 cgtctccttc ttcctggcaa atggtgccgg tatagcttcc tacattgccc aggccttgaa
    32821 tgaccatggt tttaccggcc actccccggt ccattttcag ggctttcatt tcaggtaatt
    32881 cgtcgcagaa ttcccggatg ccgtaatata ctcctctccc ggtcgcttcc gtacggccac
    32941 ggataccaaa ctggctgacc ggtttaccgg tgacgcaggc aatgccatcc agttctccac
    33001 cccggaaggt catgtaggta tcgtaaatcc aggccatttc ccggctgctg gtcccgaaat
    33061 ccggcgccgg cacatcgatg cctggcccaa taaagttttt cttgatcaat tcaagggtgt
    33121 accgcctggt gatcctttcg atttcttccg gcttgaggat gaaaggattg attttaaccc
    33181 caccctttgc cccgccgaag ggcacgtcga caatcgcaca tttataggtc atcagggatg
    33241 ccagggccat gacctcctcc tggttgacct ccatgctata gcgaatccca cccttggtag
    33301 gctggcgatg atgactgtgc tgaacccggt aggcttcgat caccacatat tcgtctccga
    33361 tttttgcagg gaaagtgatg tgatagactg cattacaggc ctttatttgt gccagcaagc
    33421 ccttgtccag gttgctgtgc agcgcggcct tgtcaaaatt gcggcttacg gattcataaa
    33481 aactaatttg gtttttcttg ttcattatgt ttggtcattt gatcttcaat gctttttaat
    33541 tttttttcca ggcgaatgag atagaataag accaggagcc agattaccag cgcaaccacc
    33601 gctacggtat aaattttacc ggtgcttctg aaaaaatcgg catcctgcat caggaggaga
    33661 aatatcggtg gaattgaaaa attcatgatt aaactaatat tctaaataga ctaatctatt
    33721 ttattgtata acaatttgat acgatatctt atctgggaaa tccaaaaggc gatacagata
    33781 taagcgatca ccgccggata aaagaccagt ctcatggtat tgtccaggtc ctggctgcca
    33841 aaggccgggt ttcctccact gcccgggtgc agggagtcga acctgcgggg gatgataaaa
    33901 atcaggggga tgagcaggac aaaggcaaag atgttataga tgcttgaaat ccgggccctg
    33961 gcatccggat cgcccagatt gctgcgtatc aggaaataac caccgtatat gagaagggat
    34021 atggccgtca tattttgttt cacatcgaaa ctccagaaag cgccccaggt gaacctggcc
    34081 catatcgccc cggtaaccgt ccccaaaatg ccaaacaaga caccagctgc gaccagggat
    34141 tctgttttcc attcatcctg tatttctttg aatctcaggt acctgtaact ataataaacg
    34201 gaaaacatga aaaccatgac catggcaaac cagaggggaa catggaaata ggtatttcga
    34261 atggtttctc ccaaaatgtt ccggaaagga taattaaaat tggattcctc tcttacggtg
    34321 agcatttcct ttgaccattg gccggcggcc tgaaatccgg gtgttgcatg ggccacatcc
    34381 tggctcaggc tgaccgctga aggcaatacc agggggccgt ccggggtatc catgaccagg
    34441 gagagatcga cctgattttg catggaatcg ggaaaaacag gaatcgaaaa aaccaggctg
    34501 gcatgtcttt catcctggac caggacctga tcggcctcca gatgccattc accctggcgg
    34561 agccaggctt tgatcttgcc ttcagaatta aaccgcgtat tatacccggt aatggtcaag
    34621 gtattggtat tcagcgattt gactataaaa ggattgatgt taacgatacc ctgcccaagg
    34681 ggtaccataa agcccatcag gatcacgtaa ggcataatta ttatcgccac ccacttccac
    34741 caggccaact ttaccatatt ttgttttaat cccgccaaag gataggaaat aaaaataccg
    34801 acagtgcaaa ggtaatcaag ttcatggcga agatggacag gaggtcctta taataattac
    34861 tgtcctggat caattcgatc gccacgaggc acagacgact gcctgccatg aggatcggga
    34921 aaataagagg ggtagccaat atggtcatca ccaccgcctg gtccccggac ttggcagtaa
    34981 tcagggaaat aaaggtaaaa atcaaggata ttccactggt actcaatacc atagttaaaa
    35041 tgaacaaggg ccaatgggta acgggaaggg taaaaaacaa accgatcaga agaatagcca
    35101 gggcattgag cccgagcagg aggatcaggt tgtagaggac tttggaaaaa taaacggaca
    35161 aaggcctggc cagggtataa atataccagt gcctttgttt cacttcccgg ctgaaacagc
    35221 cttgtaccgt attgattgac aggaatataa gtacaatcca caacagtccg gtccaggtca
    35281 ttcgttccac cctcatcagg gaatagtaca gcaccagaat gatggaaaac aagtacaaac
    35341 tgatcgaagt cagagcctgc ttattgcgca attcactttt aaaatccttt cccaataatc
    35401 caaagacctc tctcagcatt atttttcttc tcgggcaaaa ttaacagtat tttataccaa
    35461 gtcgattaaa attaagaaaa agtggcatct taattgtctt atatattaca caataaatac
    35521 atttgtttat atttgtttaa caattctttg tcccatgaaa cagtgtatct acccaaaagg
    35581 taaggcccag attatcggca tttttatgct attatcggct tattccccgc ttattgctga
    35641 aaattatatc agtgtcaaga ttaaaagcgg ggatggtctg tattccctgt tcaggcggta
    35701 ccagcttccc gccaacagtt gcaatatcaa ccagtttaaa caaatcaacc agttgaagga
    35761 tataaacaaa ttgctccttg accggaccta taggcttccg gtcctgctca tgccgttcaa
    35821 tgggaaaagt gtaagcactt ccgttccggg tattgacaag tcaacggtgg ttagaattat
    35881 caattacaac aaaaccgttt ataccaataa gcttagaaaa ttgccttaca accagagcaa
    35941 attggtatgg atcccttatc atgaactgaa ttgttcatcg ggatcggcga tgaagtcctc
    36001 ttccgcttcc gtgcggacta cccttgcggt gagttctgcg aaaccggcat ccgccgtcaa
    36061 gccggcggca agcccttcag taaagagcgt gaacagcaag cctgaaacgg tcaaagtaga
    36121 aaatgacctg gaatctaccg ccgagaccac ttccaatctt gaacccgcgg tcagcgaaaa
    36181 agcagggctg cgtacccgta ccgttccgct cttcggacca gattttaagg agatccggat
    36241 cgaaagcgaa ctcctgaaaa ataaagtatt ctacctggtc agcggtcatg ggggacctga
    36301 cccgggcacc atgtaccagg gccagaacat gacgctttgc gaagacgaat acgcctatga
    36361 tgtgaccctc cgactggcca gaaaactgat ggaacggggt gcaaccgtac acatgatcgt
    36421 acaggaccca aacgacggca tccgaaataa tatgtacctg cccatggaca aggatgaaaa
    36481 atgcattacg ggttaccccc tggtattgaa ccagcgcctg cgccttaccc agactaccga
    36541 tgccgtgaat gacctgtacc ggatgcaccg caaacaaggc attgccgaga aggatcaggt
    36601 agccatccat atccatgtcg attcacaaca caaggacctt cggaaagacg tctattttta
    36661 ttaccaggaa gacaataaac attcgctgga aatcgcccgg aagattcaga aaaccctgga
    36721 taacaaatac caggacctgg cgggcaggag ttacaacggc accatcagca gccggaacct
    36781 gtttgtcatg cgcaacacca tcccttcaac catttttatg gaactgggca atatacagaa
    36841 cacgatggat cataaaaggc tcctgatctc ctccaaccgc caggcgctgg cggaatggat
    36901 ctatgatggc attgccggag aataagcgtt cctagggatt tctttgaacc ttctcaggcc
    36961 aataacaaat tacagcccct gatatcgctg cggtccaacc tgcccagcac ttcaaaccgg
    37021 ccgtccccgt aaacccggcc gaggtcatca gtcgcaataa aagcgcagga atccacattg
    37081 gcgaggtcga tgatgtttaa ggcggcctgc tgcccaggct gttccctgct gagcggatcg
    37141 ttgatctgcc ggggaaccac cctcatgcag gggcccggaa caaataaccc ctccgaaagc
    37201 gcataggcct gggacatcaa ttcagtcatc ccgtattccg aacagaccga ggtcacgcca
    37261 aaattttcca tgatcttttg atgaagttcc tgccgggtca tttctttttc cctgcctttc
    37321 atcccgccgg tttccatgac gagggtatcc ccggaaagct gcaagcgcat gggccagttg
    37381 tatagcgcat aactcacccc ccagagaatc gttggttctt ttatggtttt ctccagttcg
    37441 acgcttaaac ctgcaggatc ggagtgataa taccccccct ggcccctctt catgaattga
    37501 tccagcatat acagcaggga cgacgcttga ttttccgcat aggatggaag gactgcgagg
    37561 tgcctgaatt ggccaggctg ctgaccgaag atatggctaa aacaaacaga ggccatttca
    37621 tgataccaac cgggttcccg gaggaaatgc cggcttcttt gctggcctgt ggtggcgctg
    37681 ctttcaaata ccagggaggg attgtaggaa ccggtccgga cttctttggt tttaaaaaac
    37741 tcgatgggca ggcaggggat ttcttcgcaa gtattgacgg caggcgcact gccataaagc
    37801 aggtcaaggt atgccctgta gaccgggtta aaggcatact gggcttcgta caacctcagg
    37861 gcaagctcat caaacacgaa gcccccaagc cggataaggt ccatgatccc cttccgttcc
    37921 gtttcccaat gcatgctgca atgatcgtaa aaatatgcca ggccaaagcc ggtacgtgaa
    37981 ccaaaaaacc cggatattta cctgttcatg atgggaaaac gaatcctctt tctgacctta
    38041 ttcctggtgg tcgtcctgat ccaggcctgc atcattgcgc cggattatcc gatcatccct
    38101 gaaatcagtt ttgaaggggt caacaagacc gtggtcgatc agggcagggc ccgggacgat
    38161 tccctgtaca tccggattgc cttcacagac ggggacgggg atatcggaga cgaacaaaac
    38221 aaagcgaata tttttatgga ggatgaccgg gatggaatgc tggtgaccta cagcatgcct
    38281 ctgatcccgc aaaaaggggt ggccaacggg gtttccggaa ccattacgat ccttcatact
    38341 accctgttca acgtctgctg ttattacccc aatgccgatc cctgcagcat cccggacaaa
    38401 cccaccacgg ataccgtgac ctacagcatt tatattgaag accgggccgg acataaaagc
    38461 aatgtgatca agatgcagcc catcgtgatc aattgcaaat aaacagactc tcctattttg
    38521 cgtaaacccg ggtcacgaag ggcccttcca taaagcggta ggggtcgatc tggctttgga
    38581 ataccgggcc tttccagttt tccgtcaaca gcatcctggc gatttccgcc atcggagcgg
    38641 ccgtggtggc ctggatcgcc tttaacttga gctcgcccca gttgatctgg ttgatccaat
    38701 aagacctttc attcagccaa agggtattgt tggcatccct gccctgggcg gaactgtaga
    38761 tcaaaacaaa atcttcctcc accatcggta tgttttccag catgatgcgc tgcaattcat
    38821 cgattttatt ccggttttga ggaatggtgg atgccgagat cttgacccag tcgtaatggc
    38881 ccgggaatcg aagggtttta taatccagtt ccgccaattt tcctttgaaa aaatcgggca
    38941 ggtcggcggc ccccccggaa gtgaggtccg cttcaaaatg aatgccgtcg atgatcagcc
    39001 gctcccttga agacagcgcc ggcaccaggg ttttctggta attgcggatg accacacaat
    39061 cttccaggta ttccgtagcc actcccacgg ggctccaggt aaatccgtaa aaatgagggt
    39121 cccggcaatt caacggcagg gcccctaccc gcataacgat cttatccgcc ttttccacgg
    39181 ggtattccct gaagagatcc aggtaaagcc ggtgcgcaag gatatcgata aatccgggcg
    39241 ccagtccggt ttgcagtaca aaggcggtat ccgccccctg ggcgatccgg atcacttctt
    39301 ccgtttcctt tacatattca gtgaggttta cgtaatgcag gttgaattgc cgggccagcc
    39361 gggcgatgcg gggcgcctgg gtgccgggta gacaatcgag tacaatactg ctcgatctca
    39421 ggatggaatc catctcttca ttgctccccg ccgcaggcat ggagaaggtt ttgacctgtt
    39481 ccgtattcac ctggccggat cgcaaccatt gtaaggcgct ttcgcaggcc ctgggatttt
    39541 gatctccgat aaagacacgg agcccgtccc cgtaatgatc cgcaagcatg agcgctacgg
    39601 ctctgcctat cccgccggca cctgcgatca caatctgcct ggtttcgttc attgatttgg
    39661 tttaaagtcg ccgcaaaact aagcattccc ggaagggatt gagcagctaa agttattttt
    39721 accgctccaa ttgtattaga attgcattca tgaatgagga gaatctcaag gcgctgctag
    39781 cctccctgga aggatcgctt tcctgggacg aaaccaaccg gaccatttac gctacggacg
    39841 cctccgtata ccggaaatta cccgccgcca tcgcccttcc aaaatccgtg gacgacctga
    39901 aaaaactgat ccggtttgcc catgaaaata aaaccagcct tattccaagg accgccggga
    39961 cttccctggc cggccaatgt gtcggggagg gcatcgtggt cgatttctcc gcccatttaa
    40021 accaggttct cgaaatcaac aaggaggaaa aatgggtcag ggtgcagccg ggtatggtcc
    40081 gggacaaatt gaaccaggtc ctggctcccc ttgctttgtt tttccctccg gaaacggcca
    40141 cggcaaaccg agctatgatc ggcggaatgg tgggcaacaa ttcctgcggg gccaattcga
    40201 ttgtatacgg caccaccagg gattatgtgc tggaaataaa agccctgctc agcgacggga
    40261 gcgaagcggt attccgatcc atccgccctg aggaagtgaa ccggaaaacc ggtcttgaaa
    40321 aaaaagtgta tgaccacgtt ctggatgaac tcggccacga agaaaccgtt catgagatcc
    40381 ggagccagtt tccgaaaaaa aacatccacc gccgcaatac gggttacgcg gtggatatgg
    40441 tcctgaacga actggaaaaa ggacaggtcg acctgacccg cctgatctgt ggatcggaag
    40501 gcaccctggt gatggtcact gaactgaccc tgaaactggt ggaccctccc ccgcccttta
    40561 acctgatcgt ggtaccccat ttccattcca tacacgaatg catgaaggcg gtggtggcca
    40621 ttatgaagca ccgtcctttt gcctgcgaac tgatggaccg cgtgatcctg aaatgcacgg
    40681 atggccacga agtgtacaag acatataaat tctttgtgga cggggacccg gaggccttgc
    40741 tcatggttga attcaggggt aaccaccagg aggagattga acatcaggcc cgggagttga
    40801 tcgaagaact caaatccctc tccttcggtt atgcctggcc cattgtaagg tccccggaaa
    40861 ccaaaaaggt ctgggacctc cgcaaagcgg gtttgggctt attggccaat atggaaggaa
    40921 aagccagggc cattgaatgt atagaagaca ctgccgtagc actcgaagat cttcccgcct
    40981 acatcgagga attcgctgcg ctgatggaaa catatggtca aaaacccctg tattatgcgc
    41041 acgccggggc gggtgaattg cacctccggc cggtcctgga catgcacgac ccggaagaag
    41101 taaaggaatt cagggccatc tgcgaagaca gtgcccgcct ggtgaaaaaa tacggcggat
    41161 cgctcagcgg agagcatgga gatggccgcc ttcgggggga attcattccc ctcgtgatgg
    41221 ggcagaaaaa ctatgaactt tttatccgga tcaaggaaac ctgggacccg cattacattt
    41281 ttaatccggg taagatcgtt catgctccaa gaatagatga ggacctgagg tatacctatg
    41341 aaagggcagg aaaagagccg gaaacccagt ttgatttcag tggacagggg ggattttttc
    41401 atgcctcggc caggtgttcc gggtccgccg actgccgtaa acaggcctcc atgggaggga
    41461 ccatgtgccc cagttttatg gccacccgac tcgaaaagga caccacccgt gcgcgcgcca
    41521 acgccctgac cgccttttac agcgatccat ccttgtcttc catccgggat atggattcgg
    41581 tgaaggaaat cctggacctc tgcctgtcct gcaagggttg tcgcaacgaa tgcccttcca
    41641 atgtggatat gaccctgctc aaggccgaat ttacccacca ataccggatg aagaagggcc
    41701 tcccctggcg ttccatgttg ttcggcggaa ttgaccatat aaactcggta gccggtttga
    41761 tgcccgtttt ggcaaacaaa atccaaaaca accgtatcct gaaaaaatgg atcgggatac
    41821 atccggaccg cagccttccc gccctgggca gccatagcct gagacaatgg accagtaggt
    41881 tcaaaccttc ggcatcttcc ggcaaacaac cggttttgtt ttttattgat gaatttacca
    41941 accggtacga cgtgcaggcc ggaatcaagg cgatcctttt attggaccgc ctgggttttc
    42001 ccgtgcgatt tgcaaaacac cgttccagcg gcagggccca gttttcgaag ggcctcctcc
    42061 ccgaggctaa aaaactggct gcctggaatc tccgagtcct gtcggaattt atacccacac
    42121 accaggtggt ggggcttgaa ccctcggcaa tactcagttt ccgggatgaa tttcctaaat
    42181 tgtttcgggg ggcggaacag gccaaagcag aagccctggc ggcccactgt tttaccatcg
    42241 aagagttcct tttccatgaa atgcagaagg gaaatatcag cgggcaggat tttgaccaga
    42301 cccccaggat cctcctgatc catggtcatt gccatcagaa agccctttca aaaatggaat
    42361 acacctcctt cctgctgggt ttgcccgcag gccattcggt gtcaaccata gcttccggat
    42421 gttgcggcat ggcgggaagt tttggatatg agacggaaca ttattctctg tccatgcaga
    42481 ttggtgaact ggttttattc ccggcggtcc gtgccgcatc tccggagacc cgggttgtgg
    42541 ctgccggtac cagttgcagg caccagattt tggatggcac ggccaggaaa gcatttcatc
    42601 ccgtggaaat cctctgggaa gcccttaaat ccgcatgaac taaagatcgg aagccggtcc
    42661 tctctggttg agatcgtctt tcagtttttg tttgaactcc cggatggaat caaattcctt
    42721 ccgccagctc aaacctcctc ctatccggtc ccgcagctgc ccctggttga tgtcatcctt
    42781 tttggcataa gcccttattt tgagcctgcg gtccctggtc agggcatatt caacctcaaa
    42841 atggggagtg atcagccggt tgaacagggt gctgtttata aaattggccc ctacgccaac
    42901 gctgagacgg tcctcaaaaa aacggggacc aagactgatc gccacttcat cgaaaacgga
    42961 aaggttgttg atatcgatcg cgttattgtt attgtaataa tttacgtcga aatcgaaacc
    43021 ggaaatcacc gcgttgtccg cgaccaattc ttttaaggca ttttccacca ggatggacag
    43081 ctggtttgat ataaactggg aaagattgtt gatcgtggat ccgatcaccc cgcttgaagc
    43141 aacgagttgg ttgggcggga ggaaacttcc ccagaccatc agcccgagca cttgctggtt
    43201 caacgcgttt tgatcggatt tgaggaggtt gagtttattg tccacaatat tcctgaggtt
    43261 gccggtgagg ttggggatgt ccagtccgaa actgatttca ggcttaaaga gtttgccgaa
    43321 taaatgcatg ctcaggtcga cggcagactg ggtgcggagt tctgcgcttt caatggcatt
    43381 ttcatattcg gggagcaggt taatcaggct cgaggtaagc cctttgtaat ccgcatccag
    43441 attgatgtcc gcatcggtgg ggctgccgtc ccatcggatg gtgcccccct gccggatcac
    43501 gaatggttta ttcaccaccc cgagcaaggt aaagaggtac tcaccctgtt ccacttcgta
    43561 attgccaaac atggcaatgt ctccgttccg ttcgaaccgc atttgaaggt caccgttgcc
    43621 ccggcctttc aggatgtccc ctcttttttc gtcaaaaatg agggagattt ccgcatcgtc
    43681 atttacggtg aggtcaatgc tcaccgaaag accgttggcc gccaccacgc gcggctcctg
    43741 ggtttcgctg gcggtcgagg cctggacggg cctgaatttg accagtcgtt cgctggtgat
    43801 ttcggtagtc tggctgatcg ggatataaag gaccgtgggc ctcgccgtgg tcccctgcac
    43861 atcgatgtca atccggttga agggaccggt aaaacgggca ttcacattgc cgaccacggt
    43921 cccgtaatag tccgggttct gaacttttgt ggtcttaagt acgaggaaac ggtttgaacg
    43981 gatcccggca tttaatctga accggttgaa ccggtcatgt tcaatacctc cctggatggt
    44041 accggtgttg ccaagttcgt ccctcaggct aacccccgag gcatcgatca aattattcgt
    44101 gatggtcacg ggctggttgc cgatctggaa actggttccg aggtaattga tcttgaggga
    44161 gccatcgccg atatttaccc gtcctttaaa ctccggtttc ccgcccacct tattgatctc
    44221 aaaagttcct acgatgttgc cgcgggtctg gtccacgatg tcgtcgatca tgaactcaaa
    44281 caacttcaat gggaaatcct tgaaaagtac cttgaactgg taattgtttt ttaccccgtt
    44341 tgaatttttc cccgaagggt caaaaaaacc ttcccccatc agctgcctgg agccgtccag
    44401 caaagccacg ctcaggttaa aaggctgatt ggtatttaca ttataaagat tgaggtcaag
    44461 caggccaaaa taggtgtcgt tgatataaaa ggaatcgacc cttccggata accgcacccc
    44521 acggaaagtg aatacatccg cgatttccgc gttaaggtcc agccttcctc cggcatccca
    44581 ggcttttacg ggattgaagg aatttaccca gttgatgtca aaatccgtaa actggacccg
    44641 gaggcctttg ttgtccctgc tgacgatttc aatccgttcg gtaccgtgtt caaacctcag
    44701 attctgcgtg cggagatatc cggggcggta cacaatttca ttgtttggca gaatgctcca
    44761 gtttttgtcc atgaaagaaa gattggaagg agccagggac aaatgccagg aggaatcgat
    44821 caggcggatc gccccgttga gcgagacgtt attgagcaat ttggacactt ccggggtacg
    44881 gatggaaaaa ttcagcagat tgtaagaaag gtcagcggtc aataccaatt ggggtattct
    44941 gaagttgccg ctgtagatcg aatcgatcag ggaggtggta taggccacgg ccccttttgc
    45001 ttccagcctg ccaatggcat tgaatacttt aaagtctttc cacctgaaat cgacgatatt
    45061 ccagttggcc accatatatt tttgagccga atggacggat cctttaagac ggaatggttg
    45121 tgaggcttcc atatcctgtt tcaggatcct gctcaatccc cttaaatcct ttacatccag
    45181 cgtaaagtca taggaacagg aaatcaccga atccggattc tgtttcaggg cttcctggat
    45241 aggcagggat tttaagaaat cggggaattg gtttttcaat tgttggttta gagccggcca
    45301 aatgttttta atcaggtagt tccctctgag ttcaccatcg atccagtccg aatggaccgt
    45361 gatgccccgg gttccgtccg cgctgcggtc ggcatccacc tccatgtttt gaagcaggac
    45421 cggtacggaa tccacctgga tgatgacctg gtccaaatcc accttgccaa tgagttcatc
    45481 gaaattccgc ccgatcatgg aaatttccgt attgcccgaa aaagccaggg gcttcttgga
    45541 aatgttcaac cggtaaaggt ccagttggct gatttccgct gtgaaattgt aggcaggttg
    45601 ggtattggta aaatcaattt ttccgataaa cgccaggtct gcattggcat cccgcagaat
    45661 cagtttcccg tccagcagtt tttgattgag atgaccgtca aaggtgatgt ctttataagc
    45721 gtacttttta aagtccagtt ggctgacgac ggcttccagc gtggcttcgg cggtcgcggc
    45781 agtcaagcct ttcccatccg taattttggc tttcaagctg gtttttccca attcctcgtt
    45841 ttcagcccat ttcccaagat caaaatcaat caggtcgatt tctccggaat aactggccac
    45901 ctctgcccct ttccgggtat tcagacgcat atccatcctg gacgaaccga tgtcggtttg
    45961 caagaccccg taggccacaa agtccgccag ggagccgtca aagtttcccc ggaagcgaag
    46021 cttgcctaat ttatcaaaat tggccagctt ttcatttgcg ggcaggagct gcctgaggga
    46081 ggccatatcc gtctgcaggt tttttaatcg cagggacaaa aaactttctc ccggaatggt
    46141 cacgttgttg aggtcaaaat ctccattcag actcagggta tttccgattt ttaaaaccac
    46201 ttttgaaccc ttcaggtcat tgatcgagcc ggtgagcaat ccgtccatct ccagtttttt
    46261 ggcggaattg gcctggagaa accggttctt tttaagggaa ggcagccatt cgagcagctc
    46321 cgacaccgac aaactggaac cgtttaaaac cattttccat tttacctcat cttcaaaacg
    46381 cgcaaagttt tcaaaaccct taaaatggag ctggatgctg tccttcaggc ggctgtcccg
    46441 ggtttgcaga tccaggccgt tgagctgtat ggtttgatag tccacaacaa tggatcccgc
    46501 gctcaggttg cggatgccca tttgttgcgg attcagggct gtcagtttac ccagggtccc
    46561 ttccagccgg ccctgggata agctgaggct ttcgacttca aggctgatgt cctggagatg
    46621 attggtgacg gcctgccagg aaaccaccgg ttttatatcg tagcgatcga agcgtccgtc
    46681 ccttatgctc aattccctga tctttaccgt gagcagggag tcttctatcc atttgaaggc
    46741 tttgcttttg gttttaggcc ctttggagct caagctgatg cttggcctgg tcaggacgat
    46801 ccggtctatt tcaaaccttt tgtttttgag atcaaggaga tcgacattta tcgcagcgtc
    46861 gtcaaggtat atatctgaag tggaattcgg ggaatcctgg ataaaatgaa ttcttcggag
    46921 agccagggac tccaggtcca gggaaatgcc gcctcccccg gattttttgc ctcctgactt
    46981 caggtttagt ttccgaagtg cctgctccag ggaactcagg gaatctcctt cgtattgaag
    47041 gtccttgagg gtcagccctt ccagaaaggc ccctttgaaa cggaaatctt tacgcagcag
    47101 tgaagtgatc gccatattga ggtccaccgc aaatcgatcg atatgaacca gggtatcctg
    47161 gcgggcatcg gatacatgaa caccgcttac cataaaccgg ttgaaccatt ccagggaaaa
    47221 gcggtcaatg gttacggctt ggccgagttt cccggacaaa tagacttcag ccttagtcct
    47281 tgcccaattt tgtaccggag gggcctggat caccagggcc agaaacaaaa tcagcaaaat
    47341 acaacccacg accagggtca aaaggccccg gaatatcttt cccttgaaag tggctttata
    47401 tttttctggt atggacatta atttattaac gcagcccggg cttcaataat ttctgttcgg
    47461 aaggccttaa cccctgttta accctgtcaa tcatgcctgt agatcctgcc ttctttcatt
    47521 acaaaacgga cttgctgaat ccggttgaaa tcccgcatcg cgtcgccttc aacggccaca
    47581 atgtctgcaa acttcccggc ctccagggac ccggtctggt ccgatatgcc gaggatggaa
    47641 gcattgatga cggttgccgc ccggatgcaa tccattgggc tcatgccgag ctcgtgcatg
    47701 tattggaatt ccaagccgtt tttgccatgg ggaaataccc cggcatccgt tccgaaagcg
    47761 atccgggcgc cttttttgac cagtttttca aatacgttcc ggatcaatct gccggtttct
    47821 atggctttta cgcggaccat ttccggaaaa aaaccaggta tccgtgcaga atcggacacc
    47881 gacatgcccg cggtcagggt cggcacataa taaactcctt tccggaccag cagatccatg
    47941 gtggaatccg tcatctgtga accatgctct atggtggtta cccccgcgag gatggccctg
    48001 cggatcccct cgtccccgtg ggcatgagcg gcgacatgaa ccccataatc ccgggccgtc
    48061 tccacgatgg cattcaattc ttcctgtgaa aactgaggcc tgctcccgtc tctggtcagg
    48121 ctggtcaccc ccccggtagc agtcactttg atcacatccg caccgtcttt caacatttgc
    48181 ctcacggctt tgcggcattc atcggggctg tcggccacac ccgattccgg gccgggagcg
    48241 ttgtagatcc ctttttttgc accggaggtt tggtcgccgt ggccaccggt actggaaata
    48301 gccttgaagg aagtatatat cctcggcccg gtggccagcc ctttgagaat cgcgttttta
    48361 taggctacgt tcactcctgt tccgcccagt tccctgactg tggtaaatcc ggcgaggagg
    48421 gtcttttcac caaaagaagc ggccaggtaa gccacatcgg attcggaaag cgtaaaccgg
    48481 ttgatataac ttccgcgctc gatcacggac tcgatgtgga catgcatatc gatccagcct
    48541 ggcattacgg tcttgtcttt gagatcaatg agtttggcgg aagccggagg gtccacatat
    48601 ccatgcagaa cctcccggat ttttttctgt tcgaccacca gggtcattcc gggttctact
    48661 ttaccactat ttacattcac cagattaccg caatggagat agatggtttg cgagtacagc
    48721 tgaacggtgc aaacgaaaat caggcatgaa tggagaacga gtcctatggt attctttaac
    48781 ttcataaaag gcgaatttag ctgttttcgg gggcgctccc ttgcaatttc tcccacactt
    48841 ttatagtatc gttggcggct cgcacatcat ggaccctcag cagccgggcg ccggcattca
    48901 gggcctgtat gtggagggcg gtggtgccat gcagggcgtc ctccggtgtt acgcccagcg
    48961 ttttccaaat catggacttt ctcgacaaac cgaccaagat ggggaattcc agcaatttaa
    49021 aaacgtgaag gtttttcagc agaacataat tctggtccac ggttttggca aatccgaacc
    49081 cgggatcgag aataaggtca tggactcctt ttttaagcag caatggaatc cgctggataa
    49141 aatagtccag cacttcgcgg atgaggtcat cgtattgcac cagactgttc atcgtcctcg
    49201 gcgttccttt catatgcatg aggatataag gcaatttata ctgggcggcc cagtcccaga
    49261 tctctccccc cgcccccccg ctgacatcgt tgaccaggtg gatcccatat ggggctatcg
    49321 cttccagcac ggaaacccgg taggtatcga tggacaagag gacttcggga aagcgggagc
    49381 ggatgcagga acaggccaga tgaagccttt ccttttcaac ttcttcttcc agttcggggg
    49441 cgcccggcct tgtggacatc gcacccaggt caataaaccg ggccccttcg gagatcatcc
    49501 gccctgcctt ctccaggatc tcgtcttccc ggatcgccct cgaaccttta taaaatgaat
    49561 ccgtggtcag gttgagaata cccagtatta cgggcctttc cagcgacacc agggttcccc
    49621 ggcaattgag gctaggcggg acaaaaaagg gatccgtata catgcttaaa acgaaggtcg
    49681 tcaaaatatt cacagggaga ccattaagag aaaaaaggct atatgttaaa ttgtttgttt
    49741 cataaagatt ttaaattact tttaggactt cagggcttga ccagaagggt tcccataaac
    49801 gatgccataa agtgcgtgaa tggaagaggg tccattaaaa aatggctggt gtgcttcccg
    49861 gtcttcatgg cagcgttttt ttcttttggg catcccctga aacgaattga tttattaacc
    49921 aaaaacttgt ttgaccgtgc ggattagtca tcgacagaat cagggggatc gttttgcctc
    49981 atcgcataca cgttatggta tttttctctt ttgcagtttc ctccttttca gttctttctt
    50041 tttatttccc gcccgttttg gaaagctctc tgcgcctccg gctattcagg aagaacaatg
    50101 cgtggaaccg gtctatctct acggttttga cagtaacatt tttcacattg aagaaggtaa
    50161 aatccttcga aacgattatc tcggtaaaat tctctccaac tgcggcatcc ccggcgacct
    50221 gatccaccag atttccgagc acgccaaaga tattttttca gtcaataaat tgcaggccgg
    50281 taaaaaattt gccctcgtct ccgggcaacc ctgcggcaaa ccggattatt ttgtttatga
    50341 acccaatccc tggtccttcg tgatttatgg gctgggtgaa aaaccggagg ccaaagtggt
    50401 ccagaggccc atcgtcacga gcacccattt taaagaaggc catattgaaa gttcgctctg
    50461 gaatgccatg gctgatcagg gatccaccct gcaactgatc tccctgatgg aggaagccct
    50521 gggcagccag gtggattttt atcacgtcca gcagggtgat tctttccgcc tcatctatga
    50581 aacccgggcc gtcgaagggg aaatcgtgga tatcgggaaa cttacgggcg cctattttaa
    50641 gaatgaaaac aaagaatatt acgccatcta ttatgagacc tcaaaacaca agggtttctt
    50701 tgacctgaag ggcgcaccga ctaaaaagtc ctttctgaaa tccccggtgg agatcggata
    50761 tatttcctcc cgcttcaatc cgagccggct ccatcccatt ttgaaatacc gcaggcctca
    50821 cctgggcacc gattatgcag cccggcacgg ctcgcccatc cgggcggtcg ctaacggaac
    50881 cgttacagaa gcccagcgta aaggcggcaa tggattatat gttaagatca gacacgacaa
    50941 gacctatgag acccaatacc tgcacatgtc taaactggga agcggcataa ggcccggaac
    51001 ccgggtaaac caggggcaga ccataggtta tgtcggccaa tccggattgg ccaccggccc
    51061 gcatgtttgt ttcaggtttt ggaagaacgg caggcaggta aaccatctcc gggaaaactt
    51121 tccggatccc gatcccatgc ctaaagaaga ccttcctgct tttttccagt ggagggataa
    51181 gatcatggat caactgagat cgggcaattc cctcgatgca caaaatcacc cggattttac
    51241 cgactatcca agacccatcc ctctgattaa atcctgagtc ttaaccttcg cacggagctc
    51301 attccacttt gcccgcaagg gtaaaactga aaaccgttcc tttgcccgga ctgcttctga
    51361 ccttgatctg ctgcccgtgt gcttccacaa tgtgtttcac gatggacaag cccaggccgc
    51421 ttccgcctgc ctgacgggat ctggccttat cgacccggta aaaccggtcg aaaatatgac
    51481 gcagatgccg ggcttctata cccgggccgt catccgccac ttccaccagc acctgctccc
    51541 caagatcatg aaggctgatg gtggtatgac ctccgggatt gccgtatttt atggaatttt
    51601 caagtaaatt gacaaatacc tggcgcaggt agtcgggatc cgctttaacc cagatttcat
    51661 gatccgtctc cgtcttgaga tccagggaaa tttttttctc agcggccatc agttccattt
    51721 catgaagcat ggcttccgta aaaccgatca tttcaatttc ttcccattcc agggaaagct
    51781 ggtccgattc cagcttatag atcgagtcga ggtcttcgat gattttgatc aggcggtccg
    51841 tgttttccgc tgttttccgg agataaagct gattgacctt cggatcggtc aaagccccgt
    51901 caaggagggt atggacataa ccctggatat taaagatagg ggtcttcagt tcatgggcca
    51961 cattcccgac aaagtcctgg cggtaacgct ccaggtccct cagggcgctc atttccttat
    52021 tctgggactt gatgaactgg gagacttcct tttcaacttg ttggaacaag ggtttgtccg
    52081 gataaatctg agttggagca tctaccgtcg agcttggacg gtggatcatt tcgtaaatat
    52141 gcctgacttt gggaaccagg tatttgttta taataaaccg ggtgacccca taggccatca
    52201 ggccggtaaa cagggcaatg accaggcgga ggatattttg ctgctcgctg tacaggattt
    52261 gggtaaaaaa ccattggtta aaatagacgg ccgcgcagac gacggctccg gtcaaaatgg
    52321 ccaattgcct gggggtgatg tttttcattc catttaaaag acaaatttat agcccacccc
    52381 tttaatggtc tgaacatatt tgtccccgat cttttcccgg agtttacgga tatggacgtc
    52441 gatggtccgg tcccctacga tcacttccct gccccagatt ttggcaagga tttcattcct
    52501 gccaaaaact tttccgggtt tggatgccag caggtgcagg atctcaaatt cctttttggg
    52561 taaatccacg ggtgtccctt tgatggaaac ggtaaaactt gaaggatcga tgacgaggtc
    52621 ttcaaacttc aggctttcat tggttttaat atcgctgcgc ctgagcagcg cgttgatccg
    52681 gctaagaaaa acggagggtt tcagcggttt ggtgatgtaa tcgtcggcgc ctacattcag
    52741 cgcttcgatc tgggtctggt cttcctgccg ggcggtgagc atggtgatca ggacatcttt
    52801 aaactccggt ttcgcgcgta ttttcctgca gagttccacg ccggacatgc caggcatcat
    52861 gatgtccagt acaaaaatat ggggattaaa ccgttcggcg agcttcaggg cttccaggcc
    52921 atctgcagcg acctccacct ggtatccgtt tttggacaga ttataggcta aaaattcccg
    52981 gatatcatcc tcatcgtcca ccaccagcac cttggctttg gtctccatac tttgactttt
    53041 gtggtaaatt aaaggctatt cttagggttg gcccataaat taaagattaa gttaatatta
    53101 aattttgtat gacggtcctt tattcaaacg acagattgga ggaatcctcc agctgatcca
    53161 gtcttttggt catctctttg gtgatctcga gcatgagctc cggatcggct tccaggggct
    53221 gcaggctttg tttgagggag gcgatatctt taaacccata gatctcgcag atcctttgct
    53281 gatagacaaa acgcaggctg tctttattgg ttagtacgga attgaggatg atggtttccg
    53341 ccacctggag gtccaggatg atgtcccggt attcttcttt ggataatttt cgctcgtggg
    53401 cgccacagga accggccggg caggtcaggc tgatcagaaa agccagtcta atcatcgcct
    53461 tcatccggca tgaattgttt gggatagata aagtgtttga gcgccgtatg gtcaggctgg
    53521 acgtctttcc aggagtggat attgagttgg accaccagga tgccgcaggt aggcacatgg
    53581 atatggtctt cggaaaaaag ggcggccagg taattcatgg tcgggttgtg cccgaacaaa
    53641 aaaacagtat ccacggtatc gggcagcttg cgcaggatgt cgaagatggc ttgttcgtcc
    53701 cctccataga tcagaggttc gacctgcatt tcctcttttt ttatcccgta ttgttttctg
    53761 aaatatttgg cggtctgacg cgcccttttg gcagtggaac tgatgatcag gtccggttta
    53821 aagcctcttt cggcgagtat cttggccatc aggggagcgg tcaccttccc tctcaggttc
    53881 agaggacggt caatgtccgc aagtccagcc tggtcccagc tggatttggc gtgtctgatg
    53941 aagcagacgg ttttcatggg tcgaggagct tgaagtactg tttgttcaaa atgaagataa
    54001 tattattcag gttatgatcc tgtccgcttc aaagatagag tatgggattc atctcttatc
    54061 aaaccgggtc cgataaaata cagaggaccc cgggaaaagg acaggaatgg ctttgaaact
    54121 ggcaaaacgc tgaagtgaac cagggatttt ctggattagg atcacagcag atacacggaa
    54181 agtgaatttt atatcaaaat gggttaaatc agtggaaata ttcaattctt ttatatattt
    54241 aaaacaaatt gaatatttga gccattacct ggttttcata ctgaagcgta gaccatcttt
    54301 accggctggt ttaatacatt ccttattcct tttcccttta cccttttttc tcgcggcccg
    54361 caataacatg ccaggcattc atattcaatc cgttgtgtca cgaaacaaac agtttatata
    54421 tgaaaaaacg attcgcttta ttgattttac tccaccttag tctgggggca gcgctggcac
    54481 agcgaaccgt cacgggtacg gtgtccgacg accaggggga attactcatc ggggccagtg
    54541 tccaggtgaa agggactacc acaggaacgc ttacggatgt ggatggtcgt ttttccattc
    54601 aggtccctga agaaaatacg gtcctgatct tttcttatgt cgggtttgaa agaatggaaa
    54661 tcgttgttgg gaaccagagc catatcgagg tcatccttca atcggcaagc cagttgcagg
    54721 aaatcatcgt ggtgggttac ggaacccaga aaaaagcgga tttgaccggt tcggtggcat
    54781 ccgttgacgt gccgagcatg ctcaccagac ccgcggcgga cctgacgcaa atgttccagg
    54841 gacgggtggc cggggtagtg tcttcaggct ccaatcagcc cgggggcaat ggatatgtgc
    54901 ggattcgggg gatcagttca tttggcagca atgaaccgct ggtcatcatt gacggggtcc
    54961 aaaccaacgg gaccaattcc cttaatccca acgacattga aagcgtcaac tttctgaaag
    55021 acgcttcctc cgctgcgata tatggcgcaa ggggagccgg cggggtcatt atcatcacga
    55081 ccaagaaagg ggtgccaaac aaaacccgca tcacctatga cggctattac ggcgtctcca
    55141 aagtgatccg ctacccggat atggtgaata ccgcagaatt gggacaactc ctctggaaac
    55201 aggccgcggg ggcgggaatc gctccctcct cccctcaata tggaaaaggg gcgagtccgg
    55261 tcataccgga ttatatcctg gcgggatcat ccggcggtct attggaaggc aacccggccg
    55321 tggatccgtc caagtatgtc tatgatcaaa acggctttta tcaaatagtg agggccaaca
    55381 aggaaggcac cgactggttt aaggaactca cgcagaccgc tccggcccag agccataacc
    55441 tgagcgcctc gggcggcagc gaccgggcga tttacaacct cagcctgggt tattattcgg
    55501 aagacggttt acagaaatat accttctatg accgctacag tgcccgcgcc aattcggaat
    55561 tcaaactcgg gaaacgcatc cgttttggag agaccctgtt cgggtccttc cggaaaagga
    55621 aagggtccgc ggacaatgga gaaggcgccc cctggtccca tgcctaccgc atgcaggcca
    55681 tcgtgcccgt ctacgatatt atgggcaact tcgcaggatc gaaggcgccg ggaaccggaa
    55741 acggggcaaa cccggtggct atactctacc gtgaacgaaa caatactgaa aaagatatcc
    55801 ggatcctcgg aagcgtttat gcggaggcgg acctcatcac agggctcctg ttcagaagca
    55861 attacggcat tgatttcaac aacaatttca gcaaagcttt ccgggatgtg aatccggaac
    55921 attccgaggg caacttccag accacctaca gcctgcaaag cggctaccag ttcaggtcga
    55981 ctttcaccaa caccctcaat tatgcaaagc aaatcggcaa tcacagtttt aatgtactcg
    56041 ccgggacgga aattgtggaa tataagaatg aacagttggg cggcaaccgg tccggatatt
    56101 atccgttcac cgatgaatcg ttctgggtat tggacagggg aaatccggtc ggacaaggta
    56161 atttttcgag cctgggggaa gaagccctct tttccgtatt tgggcggatt gactatgctt
    56221 tcgcaggcaa atacctgctc aatgccactt tgcgccggga tggttccagc aagtttgctc
    56281 ccgatgtccg ctatggcaat ttcccttcga tcagcgccgg ctggaaaata tcacaggaaa
    56341 actttatgca ggatattcgc ttcatcagca gcctcaagct ccgtgcagga tatggggtgg
    56401 tcggcaacga tcagatcgac gggaacaacc agttcagttt ctaccgctct gatccggcac
    56461 gctcctttta tgacatcacc ggtaccaata cttctacggt aagcggatac gaccttgacc
    56521 ggaaaggcaa cctgaattcc aaatgggagg aaacctccac cctgaacctg ggactggacc
    56581 ttgccctttt tgaccaccgc tttgaaatga acctggatct atacaataaa aaaacgtccg
    56641 acctgctggt ccagatccca cgccccgggg cggagggtga ttttaccgca ccctttgtca
    56701 atgttggcaa ttcccaaaac aaagggattg acctcatgct gacttacaga agtaaagcgg
    56761 cagccaaatg gtattatgcc gcctcggtaa atatgacggc ctataaaaac aaggtagtga
    56821 gcagcggcgt tgactttttt acgaatacca cccggaactc cccggtttcc aggacgctta
    56881 caggccaggc cataggcatg ttttatggtt acgagaccaa tggctttttc aattcggggg
    56941 aggaagtcgc cgcggcccca acccagcccg ggataaataa aaattcgccc cagcaggcgg
    57001 tgggtcgctg gaaattgaaa gaccaggatg gaaacggtgc ggtaaatgcc aatgaccgga
    57061 cgtttttagg cagcccgcat cccaaattcc aggccggctt taatgtggat gtcgcctata
    57121 aaaactttga cctgaacgtt ttcacttttt ggaattatgg caatcagatc tataacaata
    57181 ccaaatggtg gacggatatg aatggcttca ccggcaaccg gtccaggaga atgctttatg
    57241 acagctggac gcctgaaaac atgaatgcca gtctgccaaa acttgacgct aacgacaacg
    57301 tcagcaactc ggttccccat tcctattttg tggaatctgg atcctatttc cgtgcaaaga
    57361 cggtgcaact gggctataca ctaaacgagg gattcatcaa ccgcgtggga gcaagcagcc
    57421 tgaggatcta cctgcaaggg cagaatttgt tcaccatcac cgaatattcc ggccccgacc
    57481 cggacctgtt ggacgtgggt cgcggggaca ttggtttggg tgttgaccac ggcagggttc
    57541 cgaaccccag gcagtttctg gccggaatta atcttgtttt ttaatgaacc tcatgatcgt
    57601 atttataaat tcaataaatt cagaaaaatg aaaaatcata aaatatattt cctgctggca
    57661 gtttccatgt tgtttacgaa tgcctgcgtg gacaaagact ttctcgaccg gccacccgtc
    57721 ggggtattca gtgaagatgc cttgaaaacc aaggccggca tcaatgggat attgatcgga
    57781 gcctatgccg ggctcaatgg ttccatgtat ggcaacggat ccagtgcggg agcggatgtg
    57841 aacacttttt ccaactgggt ttttggcagc ctcacttccg atgatggcca aaagggagcg
    57901 gatgtcgggg gcagccggag taatatcgaa cgttatgaac tcacggccga taattccaaa
    57961 ctcaatgacc gctgggcttt ttgttatgac ggtgtagccc gttgcaatga tgtcatccgc
    58021 ctgacgcctt ccgtggaagg gctcagcgat acggaaatta aaaacctgct gggtcaggca
    58081 agaaccctcc gcggcctttt ttattttcat gccacggtca tttttggccg tttgccatgg
    58141 atcgatgaaa agaccaccga ttattatcaa aagaatgaac ggttattgtg gcctgaaatg
    58201 gaagccgatc tgaaattcgg gttcgacaat ctgcccgaaa cctttgcgga actgggccgg
    58261 gtaaacaaat gggcagccgg agtgatgctt gccaaagtgt acatgtacca gaaaaaattt
    58321 acggaagcga aggccctcct ggatatgatc atcagctccg ggaagaccac caaggggatc
    58381 aaatatgcgc tcatggccaa ataccatgat aattttaaaa ttgaaaccca gggcagcaat
    58441 acggactcgg aaagcgtttt ggacgcccag tattcggtga atgacaacgg aaggggattt
    58501 aatggcggtt tgggagaaaa cctcaatttc cctcacgggg gtctccctac ccagccggga
    58561 ggttgttgcg ggtatcatca accttcccag gacctggtaa atgcctacca gaccaaggac
    58621 ggccttccct atctggacaa ccacaatgcc caggaggtga aaaacgacca gggcattgaa
    58681 agttcagcgc cctttacgcc ccataccggc cctctggatc ccaggttgga ctggacggtt
    58741 ggccggcgcg gcatccctta cctggattgg ggtccccacc ctggcaaggc gtggataagg
    58801 gaccaggctt atgccggacc cttttctccc aaaaagaatg tttattataa ggccaatgaa
    58861 ggcaccctta ccgaaggcac cagccgccag ctatccgcca ataatacccg gattatccga
    58921 tttgccgatg tcatcctcct ggctgccgaa gccgaagtgg aactgggtaa tctggaaaaa
    58981 ggccgcgaat atgtcaacca gatccgccga agggccgcca atcccgacgg ttttgtcaaa
    59041 aacccggacg gtactccggc cgccaattac gtgatcgccg aatataccgc accctggacc
    59101 gataaggcca tggcattgaa agcggtccgg tttgaaaggc gcctggaatt gggcatggaa
    59161 ggacaacgct ttttcgattt ggtgaggtgg ggtattgcca aagaaacgct caacgcctac
    59221 ctggcttatg aggttactaa acgcgccaat ctgaaagggg ctgcctttaa ggatcacaat
    59281 atccttttcc ccgtaccgca gcgccagatt gatgccacca aattgcctga cgggactgta
    59341 accctgacac aaaacccggg ttactaaata ttcacgccaa tccggaaagc aggaattcca
    59401 aatgcccggt ttctgctttc cggataaaag cgtgttgaat tcgggaaatt cgggagtaag
    59461 gtcatttttt agaccgaccg aattttattt tcttgtc
//
LOCUS       DAOQXT010000017        57364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_580_length_57364_cov_5.908932, whole genome shotgun sequence.
ACCESSION   DAOQXT010000017 DAOQXT010000000
VERSION     DAOQXT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 57364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57364
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_580_length_57364_cov_5.908932"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2796
                     /locus_tag="PKM27_04540"
     CDS             <1..2796
                     /locus_tag="PKM27_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06561.1"
                     /translation="DGALPAGFRVWGILYSDSIAFNIGNDVSTIRAVSGCARLSENLI
                     PVFRDVPRSLDVMFSNGRVDTNICGSNSTLDTIRLKTVGSSVSKQIFVLTFFNGLILD
                     TFSTLWFPIDQLSIGNYRVYAMTYTGSVTIQKNVSRLTDKTLATGCFNTGGVPASFAV
                     DTLTVGSLVSLTSQSVFCPKDGSPDLYTVGVEDGESPFVATLLVDQNQVCRQVAPGLT
                     IDLDNNPEGTYDIMVLQYSGQLLVKPGDTVSRGGVTKFSSDCYALALNEFEVRLVEPF
                     AGSILAEGQKTLLFACPGDNLPDRFTFNGFGQSPNSYAVVAVGAGNKVLALTTGGGFI
                     DFERFPLGQSRVYGISYTGTLRIGVDSLFDGDLSTDCYSVTSNFITVLQDVAKGGTVR
                     LADGSTSYFVCPGDNLARTVPLVNTGASNSPYQYVLTSETGTVLDFIINDTLKIASST
                     LTSMRIYGVAYTGNIVIAKASNLFVSAFSDGCFNVSSNFIRVLRDAPRGGGVRLLDGS
                     NKELFCPSDTQSRILEVRNVSVSQNAYNLVLTDSNLVVLSIDTAFKFNFGGFAEGQYY
                     IYGVSFIGERSLKPGDLLDFSEFSTGCFSFSSNFVLVTIGTIDGGVLTSSEGSDLAFV
                     CPANLDLDIIRIIPQNAKSLSYLYVITDDKNIIEGFSSSDQINFGSGVINTTSRVYGV
                     AYKGVFQATVNQNALQAVYSNQCFDLSDNFVTIRKTVPPPHRIQSSTLDTVLSFCKTD
                     DRGDTVRLSTSDSVGFKTAYILLDSTSKILRISSVHSMIFDQEDTGTYRLYSSIYTGR
                     LLVTPGLTLIPGVSISDDCFSLSSNFLTISMVNEGPACVITSTKQEDWYSGFKVYPNP
                     SKEEIRVEFNLQNHKGGEGRLDLVDLAGRSVYTMPLNLQIKNRFIVPLSKLGAGIYLL
                     QIRTQNLVTSRKLVIGK"
     gene            complement(2819..3205)
                     /gene="rplS"
                     /locus_tag="PKM27_04545"
     CDS             complement(2819..3205)
                     /gene="rplS"
                     /locus_tag="PKM27_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNR06562.1"
                     /translation="MNPIQFVQEQLLKDRSSETVKPGDNVNISYKIIEGDKERIQNFK
                     GDVIKITGSGRTKTICIRKISNGVGVERIFPLNSPSIVDITLLKKGKVRRSKLYYLRA
                     LSGKKARIKEKKQALAPKVEVDAPQA"
     gene            3446..4003
                     /locus_tag="PKM27_04550"
     CDS             3446..4003
                     /locus_tag="PKM27_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HNR06563.1"
                     /translation="MINTSRQYRKVLMEARQLFIKKNKDYGTAWRLLRPASITDQIYI
                     KAARIRSVDTKKEQKIGDSIENEFIGIVNYCLMALMLLRSGEGASPDHLTRENVLAYY
                     DACAEEVFRLMEAKNYDYDEVWRSMRVSSMTDLILMKIVRIKEIEDREADSLVSEGVD
                     ANYMDIMNYAIFALILLHESHTPNF"
     gene            4007..5482
                     /locus_tag="PKM27_04555"
     CDS             4007..5482
                     /locus_tag="PKM27_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNR06564.1"
                     /translation="MTLSTLIIAFAVAGGLLTFLISQKVKQPKSWLISFLQNFCGVWF
                     IFSGMVKAVDPMGTAFKMEQYFAAFQQTFEPTWMGFIAPVFPWMAQSAVTISILMIIL
                     EILVGVALIIGFKPKLTAWVFFLLLLFFTLLTGFTFLTGYVPSNANFFEFKAWGDFEP
                     LQMRVSDCGCFGDFLKLDPKVSFLKDLGLLLPAIVFLVWTRLEHQLWTPFTRFLFIVL
                     AGLGSWGFCHYNTYVNEPLWDFRAFRVGSDIASTRKAELEAAQQVQIEYAELKNKQTK
                     AVTRISYGDYMKEFKKYPKEDWDTKFVYAESKVPKTKVSDYIILDTSGSDITQALLDE
                     KGYSLMISAYKVHGQVQEVKTSVSDTAFSDSLVITGTDTLRFRVIKEVVHRESTLSSF
                     TANAEEAALFRDQINPLVHGFLDKGVKVFAVTGGLGNSQMRDFAAQTGARYPIYQADD
                     ILLKTIMRSNPGLILWKEGKLIDKWHYRHLPSAEALERKMK"
     gene            5624..6526
                     /gene="nusB"
                     /locus_tag="PKM27_04560"
     CDS             5624..6526
                     /gene="nusB"
                     /locus_tag="PKM27_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNR06565.1"
                     /translation="MQILFARNRDAFIQEAHLPKMLDQSLRQSFDLYLFQLYLIQKIC
                     EQAKNVADKIQNKYLPTTEDKNFEAVLYENPLIQNLVQNKKLQKLFVKGEFDKWVEED
                     VFKKIYSEFAKLDWYKAYSDKSLAEKNHLETLLELYRFLRKNELFNEIVEDQLYNWED
                     DDSLIIGAIKKTLKALPASEEFYKEFMVTEEETLLFGKTLLKKVIEQEDELAAIIKPT
                     LKNWDSERVAIIDMILLKMAVCEFLYFNTIPTKATINEYVELSKLYSTPKSKDFINGV
                     LDRIMKDLTESNRIQKAGRGLLAE"
     gene            6528..8060
                     /gene="guaA"
                     /locus_tag="PKM27_04565"
     CDS             6528..8060
                     /gene="guaA"
                     /locus_tag="PKM27_04565"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNR06566.1"
                     /translation="MHEQVLILDFGSQYTQLIARRIRELNIFCEIKPYHQYQAGDHTR
                     AVILSGSPFSVRDANALRIPLDLLLGKVPVLAVCYGAQLLADCLGGSVVKSDHREYGK
                     ASLQILDLNDPFFTGVEDGSQVWMSHADTVQDLPPHFKLLASTDSIRVAAFKSDHYGA
                     PVYAIQYHPEVTHSLQGKRMLENFLFNISGMKGDWTANAFIEEQVGMIRRTVGEDHVL
                     MAISGGVDSTVAAALIHRAIGPRLHGFYIDHGLMREGESEKVMTLYHQHGYNVKRVDA
                     SGYFLKALHGVSDPETKRKIIGKSFIDVFEEEARALSGVKWLAQGTIYPDVIESISVH
                     GPAVTIKSHHNVGGLPEKLHLKVLEPLRMLFKDEVRRIGAELGIPQAILGRHPFPGPG
                     LAIRILGDITPEKIGLLQKADEIYITLLKKYNLYDSIWQAGAILLPVRSVGVMGDERT
                     YEYTLALRAVNSMDGMTAEWSRLPYDFLAEASNEIINKVKGINRVVYDISTKPPATIE
                     WE"
     gene            8017..9408
                     /locus_tag="PKM27_04570"
     CDS             8017..9408
                     /locus_tag="PKM27_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06567.1"
                     /translation="MISAPNRRPPLNGNKLLLLVSAVLWTLISSCSAAKKSTAAPKEP
                     VPAEIAKEVYNPRSGRYEPATPSNTRVDTVHWTRPAEAWITDEDLMTVGADEASVKSA
                     YRIAVLLPLSDDDLNKDLNAEGSDKFFHYYAGMKMGMEDMAIQYPNIRVDVLDVNANP
                     YAMQPILQSAALQQADLLVGPLRRDHLNEAARFSLQKDIPIAAPWNAFRTIENINPNY
                     ILLRSSLPTHCEALTRYLLENFNGEEICLLGRERFKPLMNYFQSFLTEFKGPLQKPFR
                     EILIKDDFRFGDDYQYLDSTKKVYIVTEYEDPNVVFNLLRHINLMRNNRPVTVIGMPS
                     WQDYGTDFINLFSQLGVTISASSHPDYSQEKVNEFKKLFFRRYQTFPLKEAFEGYDAI
                     QYLVQRLASSGRKFHLQEDLQTYDGLASSFRLEKVINTQEPVDDRLRNVICVENKALH
                     ILRFEEFRFNIIR"
     gene            9405..10064
                     /locus_tag="PKM27_04575"
     CDS             9405..10064
                     /locus_tag="PKM27_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNR06568.1"
                     /translation="MNGHLLERRAQKIRNLIAKRQSHFTVILENVRDPHNIGAVLRTC
                     DAVGIREVFLVYSDQREKDRFVLGKRASSGARKWVDVYEFDAIKTAVDTLRDKGYEQI
                     WATRIVEGSKSLFELDLSRKVALLFGNEHLGVSDEAAALVDGNFVIPQVGMVQSLNIS
                     VACAVSLYEGYRQRKLKGFYDDSPTLSPDEQQKMFEYYSSRELVRYKGKTAIRPPEIK
                     P"
     gene            complement(10061..10900)
                     /locus_tag="PKM27_04580"
     CDS             complement(10061..10900)
                     /locus_tag="PKM27_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR06569.1"
                     /translation="MRLRFWGTRGSLPTFNSDTQVYGGNTSCLSVENEGELIILDAGS
                     GIKPLADSELVKSIREFHILITHMHFDHIQGLGFFTPFFNSECRVNIYGPASNSEELF
                     KILTKYLSPPLFPVRIRDFKADLQFHALPRRSLRTSHLSINASFVCHPGPTLGFRISD
                     DTHSFCYIPDHEVALGVPAFPKSAEWTSGYELAAGCDVLIHDAQYDTEQYPAKTGWGH
                     STYDQAIKFAELCKVRQLYLFHHDPMHSDLKLEEMLARHKDEARNTTVSLAREGDCID
                     LPV"
     gene            complement(10897..11292)
                     /locus_tag="PKM27_04585"
     CDS             complement(10897..11292)
                     /locus_tag="PKM27_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06570.1"
                     /translation="MSSQDIELILSRQLSEYLAVPMALIDHDGKMVYYNEAAGFILGK
                     HFDEDGEIDLRDWGGRFFNHEGFTESTDLHEIPFFKVLSVRHLVQGEYYMRNFEEINQ
                     KLMIVCVPLVGLSQRELGALIFINLVNRL"
     gene            complement(11362..11955)
                     /locus_tag="PKM27_04590"
     CDS             complement(11362..11955)
                     /locus_tag="PKM27_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR06571.1"
                     /translation="MAKVIPYQAKRFQPIDFKRADDHAGNPGQEGQLDLFSQVQPLQL
                     PLAENTFELALKLDLRNDPSAEQYYLKSIEKKEHIAHSYCNLGVIAAHRQKMAQAIDY
                     FSRALVEEPRHCESHFNLANVYFVAGNTGLAVLHYEVVTLIDPEFADVYYNLGLAFLE
                     MEELKSAKDCFQKFKKLYPGHESDSVNVLLKSLSSLV"
     gene            complement(12055..12903)
                     /locus_tag="PKM27_04595"
     CDS             complement(12055..12903)
                     /locus_tag="PKM27_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNR06572.1"
                     /translation="MRSIWKGHIRFSLVTIPIRLYNAIETSQSISFRQLHKKDMGPIG
                     YDKKCKTCLESVKSEDIVKGYEYASDQFVIMEPEDLQKVRLKSTKIIDIEAFVSKDEV
                     HPALFDTPYFAGPDGEVATKAYGLLKSCLDQTNRLAVGRVVLREKEEVVLIAPSGPGL
                     MMYMLRYPHELRSIENIPQLDLHAKIDEAQLKLANVLVETMTKSFSQLELKDRYYESL
                     MEIIQAKIAGKEIVSVKEEEAPVIDIMTALKESISQAKSEIKPMVKATGTAGKQAAKT
                     SKKKSA"
     gene            complement(12957..15290)
                     /gene="ligD"
                     /locus_tag="PKM27_04600"
     CDS             complement(12957..15290)
                     /gene="ligD"
                     /locus_tag="PKM27_04600"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNR06573.1"
                     /translation="MAKSTFHNTRVGKRILKLSNLEKAIYKQEGILKAEVIQYYLQVA
                     PTLLKYIRGRPLSLIRYPDGIEGEAFYQKNRPDWTPEWIDSIELGKEKKNYLLAEEEA
                     TLVWIANLAGLELHMATCKKPEYNKPDLLVFDLDPPEEGWWDKTKGIALALRQFLSRY
                     DYTAFVKTSGNKGLHLFVPIEPDHPYEVVIEVLKNLTREFAALHPDLCTLNIRKNQRT
                     DKLFIDIQRNHASQTIIAPYSLRGKPGAPVSMPVDWNALDQLESAKAWSIHNAMEQIS
                     RHGDAWDGFYSLAVKLHPFRPVQAKPRAHNPEGSKHKSPEQLARYKQKRDFAKSPEPA
                     VSDADEYTSPNAFVIHRHHASRLHYDLRLQLDGVLRCWAVPRGMPDQPGIKRMAVETE
                     PHPVQYLTFEGIIPKGEYGGGAMWIFARGRFEITKQKKDGFYFRLYSPELEGEFRMHN
                     LKEKEWLLERVDRAQWDRNTYIPPMLASAAQDLPVAEHQFQYEIKWDGIRVGLQIQED
                     GLKIFSRSGRDITAQFPEISQAREHLNANVAVLDAEIVCLDGQGKPVFKDVISRMHAG
                     QMAIEALRKQKPACCYLFDVMYLDGRPLLKEPLLRRQEWLKDILKVKNTYRISDVFAD
                     GRQLKMAAERLGLEGIMAKKKDSLYTPGRRSTDWIKIKFRNTMDCYIVGFTAGKGDRE
                     PLFGALQLLSRNEDGSWVYRGRVGTGFDSALMKTIRKEILEPSRVHKKPVGLTMEEEK
                     NTTWVEPEHICEIQYASITSNKTLREPVFIQLKPDLE"
     gene            15477..20489
                     /locus_tag="PKM27_04605"
     CDS             15477..20489
                     /locus_tag="PKM27_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06574.1"
                     /translation="MYTSIPARRYQTRIIAANNLARVFPVLILIFFLTGLLRAQSACP
                     LNCNDQINISLDENCQRILVPDDVLENPDSCQNAVVSLTYPYGTHSYSSPLVDRTHLG
                     YTIIYRVTDTVNRNSCWGYILVEDKFPPQPVCKSIEISCFQLARFNDILGKVQDNCSQ
                     VGKAVISALYWVEYGCDSAFLGRVFRTIRTYDQWGNSSLCMDTLRIRKDSLHHIKCPD
                     HLQLNCRINCEKPGGGPELIQFSPDPGHPFYPSPEFLLQLQERDTFGSNTRTCIPRDS
                     MIVPGIRDILIVAVLKNTPFPAHSGLSGLASSDTCVALDTCVNMWTTEGSHRGGLCKI
                     SLGYKDHIVPVCGSGFKIRREWKIADWCAQRDTVCVQYIKIEDQEAPVITGGRTRYYS
                     SVKAHDCFVPIPLEALQAEDCDPVLQTYYLEYADGDKGKKIVLNGTLPASLNLTARGG
                     INGKRCFEIRLALTDPCLNKTETLLEACISDLSPPTPLCDESAQVTVDPATCWARVYA
                     KDLDNGSRDNCCNLLHFAVAPMSQVESTRAQAISKIETVCGRNEYWGKKAFYDAYIEN
                     WINCYVFNDYVDLTECGNVQLVLRVYEACGIPLYDPHTFPCSPHDWFCYNTSHLFRAE
                     FNFNWFDKHDKWFKDLTPRNCDWAPPVFCGDSLYAWLTHLKIRDHDKKEGFPGSIEFE
                     DFRHFRHIFYLSKNCLELDFTPDYVNKMKALTVFEPGNTCSKNLYHDCMVRVAVADKT
                     PPVCEKPKDLTVYCDGQSGNYTYAQAKYWSCSDGKEKGKWPYAIECRKEGDGDLSDAT
                     DPTGKPFGYYGGPILVYNHDDHGPSPADCEEPYNTWKPVYCKQWLCLDSLDTGRKTDY
                     KSFFSKAIFKNGGKPAEPAGSNAFYIWDNCQLDTAVKIEDTEYLDPCGAGWLKRTWTV
                     TDRCGNKTICDQKINAVHRSDFEVLFPPDVVISCMDSASLYPGLDQITGQVMIMDDEC
                     ELVGVNYEDTRYDIVVDGCYKIVRKWTLIDWCKYDPLQKERAPDIIVNDSLVADKNNR
                     ACVIRNLKDDGDGYMVYTQIIKVVDELAPVLTCKDSLLCTYVDGCKERFSFSFSATDN
                     CTPSGQIGYKWELDAGGDGTIEKRSIPNVKNFSEELASGQYRLTVYATDHCGNYDTCV
                     INLTVKDCKQPTPYCFNGISTVVMPRTGEITLWASDFNAGSFDNCTAKENLKFYFGNE
                     DCTGPGEPSKTFTCKELGFQLLCIWVVDEAGNGDYCTTYVEIQDHSNPRVCSVASLLG
                     LGGNVKTENAEPVQDVQVELKSPSGLPFQMITGNDGRYAFEGLQFNKNYQVVPARTGD
                     WMNGVSTLDLVIIQQHVLGVKPLQSMYKLIAADIDHSSEVNVIDLVELRKLILGIYDK
                     LPANPSWRFAEKSSTDNRYPYAIREIIEMKGLLQSGFQHDFVGIKIGDVNQSVTAHNL
                     LGTESRNNEKEWVLAVDDLVLEPGQSYEVPFRNAIGSLLLGYQFTFGLDPQALEFDGY
                     QSGSLRLDPSNFGFNHLDQGWISTSWNTEPVLSLQKDETLFTIRLKAKKKVRLSEVLS
                     IHSKLTPAEAYSSTDETKAVRLMFTGSKQVTSKMVLHQNTPNPFKLYTLIGLELPQAG
                     SAVLTITDISGKTLKAIRGDFGKGYHQIRVEKADLPASGVLYYRLAFGDQVLVKKMVV
                     LE"
     gene            20579..21091
                     /locus_tag="PKM27_04610"
     CDS             20579..21091
                     /locus_tag="PKM27_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR06575.1"
                     /translation="MKIGLLSDTHSFLDPHILTVFEHCDEIWHAGDFGSQEVIDRLRE
                     FKPLKGVYGNIDAPAIRHQFPEVLRWKVGGFRIFMTHIAGYPGKYSSKILKALSSEPC
                     DLLLCGHSHILKVMKDQRLGHYHINPGACGHEGFHLVRTVMRFEIKEDRLLQPEIIEL
                     GQRGRLPISP"
     gene            21149..22219
                     /locus_tag="PKM27_04615"
     CDS             21149..22219
                     /locus_tag="PKM27_04615"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNR06576.1"
                     /translation="MQDQGDEPMMLTDSDDLQDQLIIEVDKNQSPLRIDSFLNNRIKN
                     VSRTRIQQGIRAGAITVDGKNIKPNFKIAPLQTIRVIVPRHEAIDILPEDLDLDVVYE
                     DDFLLVVNKPAGMVVHPGLGNPRHTLLNAVAFHLNIDPRDPPEASQDLERMGLVHRID
                     KETSGLLVIAKTEYALSHLAKQFYDHTSSREYLALVWGEPVPDKGTVDLPIGRHPRFR
                     QLFSAFPERDQGKHAVTHYEVVEKLYYVSLIRCRLETGRTHQIRVHMQYLGHPVFHDE
                     RYGGDRIVKGTVHTKYKQFVDNLFKDFPQHALHAKSLGFVHPESGKWMQFDSELPVEF
                     EVILNKWRKYTSQKKLTGHGPE"
     gene            22206..23219
                     /locus_tag="PKM27_04620"
     CDS             22206..23219
                     /locus_tag="PKM27_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNR06577.1"
                     /translation="MDLNERIRLMASLGRMLREYPESLRDAIREASQQNKWFDEMNIR
                     LALSNIADHYLDEKKLSAWIQSYALNEPVPKTVGIVMAGNLPLVGFHDLLCVFITGHR
                     SLVKLSQKDQVLWPAIRELLIREDQAAADYFMIADRLKGLDAVIATGSDNSARYFHYY
                     FDRYPHIIRKNRVGVAVIFGGESQDILAAIGRDVFSYYGLGCRNVSSLLVPAGYDFSS
                     LLKSWEPFSELLDHHAYRNNFDYNRTLFIMNNIPFLESRSALLLEHPDLVSRIACLHY
                     QYYDSPEQVAGKLNAVKDKIQVVVSAQPIPGLATILPGEAQSPRLEDYADEVDTIKFL
                     ARL"
     gene            23216..23848
                     /gene="nth"
                     /locus_tag="PKM27_04625"
     CDS             23216..23848
                     /gene="nth"
                     /locus_tag="PKM27_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNR06578.1"
                     /translation="MTRSEKAKAILQILDDYFPEVKVPLSHRDPYTLLIAVLLSARCT
                     DERVNKTTPELFALADNPADMMKVPVEKIKQIIKPCGLSPAKSKAIAATSRILVEKYQ
                     GKVPEDMAALEALPGVGHKTASVVLCQAFNIPAFPVDTHIHRLAYRWGLSDGSSVEQT
                     EKDLKSLIPRSKWIKAHLQIILFGRKYCPARAHEASKCPVCSRYGVKNRM"
     gene            complement(23865..26219)
                     /locus_tag="PKM27_04630"
     CDS             complement(23865..26219)
                     /locus_tag="PKM27_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNR06579.1"
                     /translation="MHGFLKAFKSKIKVIRIAGQNRCFFLLILYVFIQSSCAINKFIP
                     EGKVLLTKNRVKLEDSELTRKKADRNRIKYVISSPEYGLGKSYLYFYFKHSDPGDTTW
                     WDRLFINKLSERPQWLDTTQIVQNQSDLIKHLNAAGYYQGSVLYEVAQRNKKASVTYT
                     VSPGRALKVKSMAYTCTNRHIQGLLDSLKPLLRMQPGLPLDEQLLYLDQGRIIHTLQD
                     AGYFQLGKANFDYFFSDSTNQSIDLKCTILSAETQEGFEPFRIGKIKVFSFDRLSNPA
                     YRTDTTINLIQYYESDQAMALRPNILEKYLKFRGDELYNLSALQQTRNTLQNLGIYRN
                     ISILPERAGDHVLDISLHLPPVKKQFFQVDLESSYVTNRDQAAGNKLFDFRVALQYRH
                     RNLFKGAEQFTVSIIPNIGLQAISKKILVPWGLNTQASLTVPRFWEIGFVRFANQIGL
                     MSDRFYENIKTTTRTRISIGNIYDLFYNFYSGRNEQSVQRETKASFGYEFSKDNRIFY
                     RFNPIGFDYLNYDLSPGFDSLAPQFLRKSFEDRLLTGIFIKEITADINNQYSNNNASR
                     ILISLETSGLEAGAVNLFSKKQLLPKISRFVRTELDARYTWRLSRTNDLGLRLATGLA
                     LPFGREDNAIPFVRQFYVGGPNSIRGWAIREIGPGIVKNEQASNNRFSFYQTGNFKLE
                     FGSEYRFDIFSILKGAILFDGGNVWILKKDPLLPGGELSSKFLSQLYLSTGMGFRLDF
                     TYFLIRFDSGVKLRTPYLQENGTHRPPTRSLRDLQYNLSLGLPF"
     gene            26198..26986
                     /locus_tag="PKM27_04635"
     CDS             26198..26986
                     /locus_tag="PKM27_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNR06580.1"
                     /translation="MLSENHAKIIRSLSHKKYRYKYNLFLAEGAKTVFDILRSGFKEV
                     QQIFISRAPDDPNSTLLMPFKEKVIEVTDREMSRISNLDTHPEILMTGSIPPPMVPEG
                     SYFETGIHLYLDQVQDPGNLGTILRTAEWFAVRSIGFSEGCADIVHPKVIQASMGSFC
                     RVKYWCGAISALSLDDHYPVIGAALEGESLYQNFLPDSGLLVIGSEGQGISKQVRTRL
                     TCTVRIPAYQALPESLNAAMATGIILAEWQRQLHTRNDKGQPIA"
     gene            complement(27022..28089)
                     /locus_tag="PKM27_04640"
     CDS             complement(27022..28089)
                     /locus_tag="PKM27_04640"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNR06581.1"
                     /translation="MIKRFIYILLGAGLLISGLNCAGTGSKGLTIKGRFSDASNLRIF
                     FDQLPIVTQKVEVVAQSDIGSNGKFSLSTDVPMEAGIYRIRVGSRNLTLILDGSERVI
                     EINGTLNDMDNGSCSIKGSDESIKANELAGKLFRREIGMNDLKKAVAATDNPLTSLFF
                     VLGTINETREDIDLLKNIVAKLREKHPKSAYTTDLDNIATTLEKNLMAKESAMLIKEG
                     AEAPNIALPNPEGKVIELNSLRGKVVLLDFWASWCGPCRRENPNVVNVYNKYKDQGFT
                     VYSVSLDRPGGMENWKQAIAADKLSWPNHVSDLQFWNSAAGRLYGVDAIPRAYLLDRE
                     GRIAGVNLRGPALEPAVKKLL"
     gene            complement(28136..30382)
                     /gene="purL"
                     /locus_tag="PKM27_04645"
     CDS             complement(28136..30382)
                     /gene="purL"
                     /locus_tag="PKM27_04645"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNR06582.1"
                     /translation="MLATAETNLTMATALGLTPEEFEKIQQILGRLPNYTELSIYSVM
                     WSEHCSYKNSIKYLKTLPREGKSLLVSAGEENAGLVDIGDGLACAFKIESHNHPSAIE
                     PYQGAATGVGGIHRDIFTMGARPIAALNSLRFGLPDTSHMKNLVRGVVKGIGNYGNCF
                     GVPTVGGETYFMDCYQQNILVNAMSAGIVKVGETISATAHGAGNPVFIVGSATGKDGI
                     HGATFASADLSEDSAEDLPSVQVGDPFQEKLLLEASLEVIRSGCIIGMQDMGAAGITC
                     STSEMSAKGNSGMVIHLDKVPLRQTDMQPYEILLSESQERMLVVCRKGEEKRLMEIFE
                     KWDLECAEIGRVTDSPYLEYYFHGEEVARVPAHSLVLGGGAPVYDRSFKAPGYLEKIA
                     GFDPRQVPQPDHLGEAASILLGSLNIASKKWIYRQYDSTVRTGSMNTVEPSDAALVWI
                     RGTDKALALTVDCNSAYVFANPNTGTQIAVAEAARNIVCSGGTPLAITNCLNFGNPYD
                     LEVYWQFVEAIKGMGIACRAFETPVTGGNVSFYNQSQVGDQTIPVYPTPTIGMLGLLQ
                     ADQKTNLSFPYAGLEVYLVGPVTDDLGSSEYLRQIHGIQYSPAPWFDLSMELQVQKAV
                     SKLIKNKFILSAHDVSEGGLLVCLFEACLGGNLGFDLVSMPGIRKDSFLFGEGQSRIV
                     IGVDPIREDDVVQALAGDLIPYIKLGRTRQDGIFTLDNEPFGSVTDWNKQYASSLEHL
                     MESQEQPS"
     gene            30613..31278
                     /locus_tag="PKM27_04650"
     CDS             30613..31278
                     /locus_tag="PKM27_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06583.1"
                     /translation="MKNIIFLLLIITPLSLLQAQIQKDHMLSVTIDGKEFTTQPQHIK
                     VGAYRYITGNAVKPDQSLRIWIGSWDGGFVRESGSYLIVNGDKPDTKENFKTAQDLGK
                     YKGIAAIKYVLETKSPRMEYHVGYSQNNDESLDVKIGEDGYMEFTFHCTLAGTWWKEK
                     TSATVFGGLGRIVDKMEDKAVTSATGFEQDIDPEGNGYKKQKTTDKIELKNGVVKLKM
                     KAE"
     gene            complement(31283..33526)
                     /locus_tag="PKM27_04655"
     CDS             complement(31283..33526)
                     /locus_tag="PKM27_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNR06584.1"
                     /translation="MRRKTRIPGSLHTEYGPVKMKNSFLVFFSWFSCSCILNAQDQVP
                     AWNPESHSSSGRDWLVTPITEPAGVFTQNQGKELVLYNGLLRRVFRLQPNLACIDFTN
                     TTNQQQLLRATSPEARLVLDGETFNIGGLYGQKEKAYLRAEWMDSLSGHENDFQFTNY
                     SIAGISPRINWKTSMWHMEREKASGKTVSFEYRHPRFQGLKVSVHYDLYDGLPLICKW
                     VSIRNDGRQTVRIDRVINEILAMVEEESAVIGKPDQMKTPQGLYIESNYAFNNAMRYA
                     LSDQTTHWKTDSSYTSQVNYNYETPCLLEVYPTHGPGIELPAGAYFESIRTYELLLDS
                     YDRERRGLAVRKMYRVIAPWTNTNPIFMHLVSRSDEEVRQAIDQCAATGYEALILSFG
                     SHCNIEDTSAANLIRWKTLADYAHSKNIFIGSYSLFSSRRISDEEDVINPATGKPGGA
                     FFGNAPCFGSAWGQAYSARLKTFFQATGFDLFENDGPYPGDLCASKNHPGHQGLEDSQ
                     WRQMEMQKDLYRWCTERGIYINAPDWYFLDGTHKIALGYREVNFSLPREQQVLLNRQN
                     IYDGTWEKNSSMAWGFVPLTKYHGGGPEAVLEPLHAHLEDYEKLMTQYYGAGVQACYR
                     GPRLFDTEETKSMVIRIIGWYKIYREILNSDLIHLRRADGRDWDGWLHVNPGLKDKGF
                     LQVFNPLNHELEREIRIPLYYTGLTTKAILAEKDGRPVKYRIDRDYSIKVRVRIPAAS
                     STWFVIR"
     gene            complement(33563..34330)
                     /locus_tag="PKM27_04660"
     CDS             complement(33563..34330)
                     /locus_tag="PKM27_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR06585.1"
                     /translation="MNVIITGASSGIGKAAAKEFARHGHSLILCARRQDRLDNLAMDL
                     RKSFGVKVHNLSFDIRNLAETESAIGQIPDEFKPIDVLLNNAGLASGLDYIHEGSLED
                     WDLMIDTNVKGLLYITRLISPEMVARKTGHIINICSTAGKDIYPKGNVYSATKAAVDM
                     LTKGMRLDLYTHGVRVSQVAPGMVEETEFARVRFHGDEKKASIYEDFNPLKSSDVAEI
                     IYFVASRPPHINIQDVLVMGTQQAGSNFTDRSGRKFD"
     gene            complement(34327..35646)
                     /locus_tag="PKM27_04665"
     CDS             complement(34327..35646)
                     /locus_tag="PKM27_04665"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNR06586.1"
                     /translation="MNRLCLFSLLVYTLIGGCIRPKESYTSLAPGIWRGALKLSTIPT
                     LSLKEEIERYENEGTQPVYDPEDELPFLFEVFYRPEDQKPYIEIINGSERIRIDEVYT
                     GRNRNTAEDTVWIEFVAFDSHIRGILKEGIIQGEWVARNRVNYSIPFNAHFGKKDRFQ
                     VVPSDPVSDLTGTWECLFDLNLEKTEKAIGEFKQNGASLTGTFRTETGDHRYLEGTVT
                     GNKFFLSAFDGSHAYLFFGKIAGDSLLGTFKSGKHFLSVWTAVRNPNATLSSATRRNM
                     ETGLPVHFNFLNPEGETRSLADFDQDIKILQIMGTWCPNCYDETRFLKEYLQKNLEPD
                     IAVIGLACERHRDTTKALNAIRNYRKKLEVPYEILLAATTTSKAVVSKQLPFLDSILA
                     YPTLVILDKNNKIHSIHTGFDGPATSKYPEFVREFGETIQTLKEKIK"
     gene            complement(35643..36182)
                     /locus_tag="PKM27_04670"
     CDS             complement(35643..36182)
                     /locus_tag="PKM27_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06587.1"
                     /translation="MVLENLLGIGSRVKHPAYGEGVIVRLHPVAYDVCFLHYGIKGVG
                     KEYTAWEIIEKLPADEAVTYTEAEKSLARILRQWSDITTITPIGDKWDKGLLVLHPSE
                     PGLKPKEMPIEAFFHKIVMVRDRLRVLEQKINAHPKMTDAEKVEMQQYITRIYGSLTS
                     FNILFKYKEDTFVGEKSES"
     gene            36219..37733
                     /locus_tag="PKM27_04675"
     CDS             36219..37733
                     /locus_tag="PKM27_04675"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNR06588.1"
                     /translation="MTYYYFKQRILLACVAGFLGVTTLFAQKTVKKINKKVDQATIQK
                     DMMYLASDQLQGRKTGEAGNHLAAAYIAGEFRKAGVRMIPGMKDYFQSIPFQKFIPPK
                     QGEVMVLGSDFTLGSNLVILYGGPANTKTEAVYADYGWIDESAGRDDYKGLDVKGRIV
                     LVQGGLPQGGSPTEIFASMAKKREMARSRGAVGLLEIYNLSFPWNFFRSYFSRERLEV
                     VPSPSTENDSFFHAWLQLPSGAKPSLEIGKSFPMQINTSGALSSPVQADNVLGYIPGK
                     KRKDEYVMLSAHFDHVGTRAGQVIDNDTIWNGARDNAWGTVGLIAAARYFAQNPPDRS
                     ILLAAVNGEEMGLLGSKYLADHPVLPWKQVIFNLNSDGAGYSDTTIVSVIGLNRVGAA
                     AEINAGCEAFGLATFADPAPEQGLFDRSDNASFAALGIPAPTIAAGLRSFDETVRKYY
                     HQSIDNPDNISYSYLTRFTKAFVNSANNIANMKEKPKWQSGDKYEAAAKKLYGY"
     gene            37963..38985
                     /locus_tag="PKM27_04680"
     CDS             37963..38985
                     /locus_tag="PKM27_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNR06589.1"
                     /translation="MKRILLLSLFFAGVIILTPKNIKAQDLHYSQFYNAHVNINPGLT
                     GIFNGDVRIAANYRNQWKVLPIPYMTVTGAYDMKIYGKPDRKDFFGAGLVFNYDQAGD
                     SKLNLATLGLNGSYSLPAGNNSFFTIGAQLAFSRRAFSLDDLTFDEQFDGLKFDPNRT
                     ITEAFDRRSVFVPDFSAGLNYRFQKSSRTKADIGVAAFHLNNPRNPFYTDDKSVKLPP
                     RFAFNALVQAKVANPVDILVHGLVQFQKPHREYVIGAGPKFYLNQKRGKNFAVGLFGM
                     YRFKDALAPAFHLYLNELQVGFSYDVNTSAFKTVTNKRGGPELSLIYTITKVKPLSKF
                     KACPIF"
     gene            38997..41420
                     /locus_tag="PKM27_04685"
     CDS             38997..41420
                     /locus_tag="PKM27_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06590.1"
                     /translation="MNWKKLYIPLLLALSVYFPGQSQTYKAFVKAADEAADKKDHYSA
                     LVYNLNALEFDSTRIDRLHAAAEAARNINSYKVAEKYYQKVVELDKKETYPLDKFYLA
                     EMKQRQGKYPEAKEVYQMYLAENRGDDAYFTQKSEKEVKACDWAADQVVQTQENVSIT
                     RMGDDVNTGYSEFGALRKGDSLFYSSLRFEMPGDPSYPRKTYSNLLLTVKGVASEALA
                     PINSPLTHTAHTAFSEKTGRLYFTLCEYLNSSDIRCDLFYRENVNGQFGEAVKLPEPI
                     NLAGFTNTQPNIGLDPQNLQETLYWVSDRTGGKGKLDIWYAQVNPDGSFSAPANLASI
                     NTAENDITPFFHNGSQELYFSSEGYIGLGGYDVYKSILRNKNWSEPEHQGVPINSSFN
                     DVYFSLAPDSRRGLMSSNRLGSAYLDELQEACCYDIYDVDLEPTRLDLEVTTFDRHTL
                     EALAGATVRLKNLTNPGVPDVVLTNPTANDFKFALERNKDYLITATREKYRPDTVSVS
                     TFGISRSQTLSKKVYLETDIIDLNVLTFDKKTLEELQGAEVTLIDLTDSSAVTKINPS
                     GNDFHFPLIRGRQYRVIANKKGYNPATLLLDTRAIQANTITEKMYLDIGDIYSFLPLI
                     LFFDNDQPDLRSYQPRTEKLYSETYPPYYARKEEYKDQWASKLKQEEIPASDRDYENF
                     FNDRLRKGNNDLFRFLDILIGMLERGDKFEIFLKGYASPRASERYNLILGQRRIFSVK
                     NEFLKYREGVLNQYLKNGQLKIAEKSFGETTAPRRVPDSITDQRLSIFSLDASQERRV
                     EIVEVKPSK"
     gene            41429..45349
                     /locus_tag="PKM27_04690"
     CDS             41429..45349
                     /locus_tag="PKM27_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNR06591.1"
                     /translation="MKKIYMPIIMKTYRSLLWSLGTLVLILSAQRAQATHLVGGNISY
                     RCISKDIYEVTVIVRRDCLLGDPEAYFDNPASIGIFDKNGELLQFLGVRGQLLLKLQV
                     DDTLTPAITEKICTINGSQSVCVHEATYKGSIHLPYRAGGYYLMYQRCCRNMPIQNIT
                     EPLETGGSWVAYISEEAMTACNTNPVFKNWSPIFLCAGSTFNFDHGATDKDGDSLVYK
                     LWTPYTGANRTFPLPQPPNKPDFNPVPWQAPYSEADMMGGDPFRIDSRTGMLHAVPNT
                     IGVFLLGVQVEEYRNGKLIGTVYRDFEVQVIDCSNLIDAKISAPALQCDELAVQFKSE
                     SINATHVRWYFDFDRNLNATSTEINPTYQYTDTGTYRVALVITKDSACFDTAYHVVHV
                     KSSASIKAAFGVKTGPCKDGFIPVSLTDQSTGVDPTGVYTWTVTYGSTTLTSSEKNPV
                     FSVPNGVLAVIRLDIVEEGSGCLASSTQTLLATFIQPGDHLDSLSVCTGDTVIVNFIL
                     SDSIRGKYDYTWDPSPVIIGGANTPEPVILSPVDQEVYIYATVDNKDGCTGRDSILIR
                     SLHKPKLAFTFSNVCGSLLVKVINQGDTLDHYHWDFGDGTTYSVRSPEHTYTIAGRYT
                     VTLSTADDCGTSLSKTFDIGNFPCLELKDTIAGCEGRTLQINPFANPLHGYLWRPTDK
                     LSDLNIPNPSFVMDSSRIFIADIYDLNTGIGLGTHTIFVINLLSDEIDKIRDTVLACL
                     GQGVPLNPEGNPALNYNWSPAQFLDNPTSANPLATVSSQTVFTVMITNPADSCVASSQ
                     VVVVVPIAIAAEIIPDSLEACLNIPVALNPNGIQDPNLEYLWSPADKLDNPASVNPKA
                     TIDSPTVFTVTISDKRFENCQITKSVVVDIPLIEEVDKIKDSLTVCAGVSTPLNPGGD
                     PRFVYEWSPAEGLSDPNVPNPTVTPVATTVYTVKVTDTQNGGCFLTKEVKVIVPPGFT
                     LVPAFRDTVSCNPTGFTLKVSSDNSKVTFEWFNPAGASIGTGDTLNVNPTQGTIYRVT
                     GTDEFGCQKSDTVRVIPAPVRIAISVPGGGLVCQGDSIRLTVTNQNPAQVLTYSWTPT
                     TDILSGANTASPLIRPTASRKYFVTATNQQGCSAVDSAEIRFSPVQEVTVTANPTTIV
                     ISQESQITATNVPGYTYRWSPAEGLSNPNIPNPVAKPTQTTTYTLTVTNPDGCEQIRQ
                     VTITVQTLPCARPNIFLPNAFTPNGDGKNDVLYLRGVIVESMSLIIYNRWGEKVFESR
                     NQSVGWDGTFRGKLLDPDVYAYYLTVNCIGGEVFTEQGNVTILR"
     gene            complement(45411..45740)
                     /gene="yajC"
                     /locus_tag="PKM27_04695"
     CDS             complement(45411..45740)
                     /gene="yajC"
                     /locus_tag="PKM27_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNR06592.1"
                     /translation="MTQLIILQASGGSGIFNLLMFPLLLVIMYFFFIRPQQKRQKAQT
                     AFQNEIKKGDEVVTMSGIIGRVNKIENEIVSLQIDTKSYVNVIRSAVSKEMTESYRKG
                     TPAKMEG"
     gene            45786..46328
                     /locus_tag="PKM27_04700"
     CDS             45786..46328
                     /locus_tag="PKM27_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNR06593.1"
                     /translation="MASVIFLTGFMGSGKTAAGRLLASRLGYDFLDLDDWIEKKEGRT
                     ISQIFASDGEGVFRDLEQKALHETGNRERMVISCGGGTPCHHNNMEWMNQHGFTVFLN
                     PSSQVLAQRLLSGKAHRPLIRDLDREGLVAFIDDRLKERMPYYTRARLQLVGIHDQET
                     VVSAILEALKPEISFPEGKA"
     gene            complement(46303..47844)
                     /locus_tag="PKM27_04705"
     CDS             complement(46303..47844)
                     /locus_tag="PKM27_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNR06594.1"
                     /translation="MNNLVHWYSNGFWLASFALILWHYFRDREGLIKLFQLLFVIGIS
                     VYAISFSFLPKSAGQKLVILSRDLLIMGVISLMFQFLREFRRLYWLAVILLYALSRLF
                     IGQWQRASVHQASLSYTENEWEILLELKDKDRKILAPIIKKYHLGIETAFKMVDEVGT
                     SLDNVVALEVPEDRENLIPAIREELAALESVTWVEENEVVSEIPVIPITPYKSNFKPL
                     TNDPHTDKQWSIELLGWNQVMSQFREGRIIAKKKARIFILDTGVDGKHEDLNKNYESV
                     ESRFDDDPKGHGTHCAGIAAAVTNNAVGISSLNPGPEYVSVSSIKVLGNFGMGTQRDI
                     INGILKAADQGADVINLSLGGRSIDSRQKAYSEAVRYANARGVIVVVAAGNDNSDARG
                     FAPANADGVITVSAIDTSSNKASFSNSVARIKNKIAAPGVSIFSTIPNNQYASFNGTS
                     MAAPCVSGLVGLMKSIQPALSTEEVYSILAETGSLSGDPESTGPVIHAGRAIDRLLKL
                     YPPEN"
     gene            47992..48924
                     /gene="trxB"
                     /locus_tag="PKM27_04710"
     CDS             47992..48924
                     /gene="trxB"
                     /locus_tag="PKM27_04710"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNR06595.1"
                     /translation="MEKHHCVIIGSGPAGYTAAIYAGRAGMDPVLYTGTEPGGQLTLT
                     SEVENFPGYPHGVLGPEMMEDFRKQAERFGTILRYERIEKVDFTGPKHLLTSEADLEI
                     EADAVIIATGASAKWLGLPSEKTFLNKGVSACAVCDGFFFRGKDVAVVGGGDTATEEA
                     LYLSKLCKQVHLIVRRHELRASKIMEERVMNAANIHIWWDTETQEIRGDQSVRSILLK
                     NNQTGQTTELEVSGFFVAIGHQPNTSIFKGWIDMDESGYLLTLPGTSRTNREGVFASG
                     DAQDKIYRQAVTAAGSGCMAALDAERYLSARGII"
     gene            48951..50024
                     /gene="fbaA"
                     /locus_tag="PKM27_04715"
     CDS             48951..50024
                     /gene="fbaA"
                     /locus_tag="PKM27_04715"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNR06596.1"
                     /translation="MISTKLKPGVIFGPQLKELYDYANAEGFAIPAVNVIGTNTINAV
                     LETAREVNSPVIIQFSHGGASFVAGKGLKNEFQQASILGAVSGAMHIHIVAKTYGVPV
                     VIHTDHCAKKLLPWIDGLLDIGEKYFKTRGFPLFSSHMLDLSEEPIHENISISKKYFK
                     RMARIGTGIEIELGVTGGEEDGVDNSGVENARLYTQPEHVAQAYEEMSKISPLFTVAA
                     AFGNVHGVYAPGNVELKPVILKNSQDYIREQYKTNSGQPVNFVFHGGSGCSREEIREA
                     IGYGAVKMNIDTDMQWAFWEGIKDYYEQKKDYLQAQLGNPEGAEKPNKSHYDPRKWLR
                     AGEENFKKRLKSAFEDLNCINRN"
     gene            complement(50025..50609)
                     /locus_tag="PKM27_04720"
     CDS             complement(50025..50609)
                     /locus_tag="PKM27_04720"
                     /inference="COORDINATES: protein motif:HMM:NF017939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNR06597.1"
                     /translation="MKLSLDIAEASPPGWIKAVMDDFDAFLRDHADCERKASAMALSM
                     VAKYPDRTAILPDLIDTALEELEHFKLVYQIMEMRGVQLPASMPEDPYIRALMALMKT
                     PVLPRFLDRLLVASLVEMRGAERFRLVEEALEDPELKRFYKMLWTSEAKHGNIYVKMA
                     LQYFDEKEVYDRLRWWTEQEGRIIASLPLRAALH"
     gene            complement(50606..50737)
                     /locus_tag="PKM27_04725"
     CDS             complement(50606..50737)
                     /locus_tag="PKM27_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06598.1"
                     /translation="MKKSGTKKSFVKKDARQMKNFFTVVIVITLVLFLLIYLLFQST"
     gene            complement(50753..52054)
                     /locus_tag="PKM27_04730"
     CDS             complement(50753..52054)
                     /locus_tag="PKM27_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR06599.1"
                     /translation="MENTDGSYFITGKAGTGKSTLLQLFKDTTRKKVVVLAPTGIAAL
                     NVRGQTIHSFFSFPPYPLGRKQIHRLSNYQLYKRMEMMIIDEISMVRADLLDQIDLFL
                     RVNRGQDEPFGGVQLVFFGDLYQLPPVLATQAERDHLSQIYDTPYFYSAKVFKEGFEV
                     RLIELHHVYRQAEFRFIQLLDRVRTHEFDPDDLDQINERAGTHELTSDFVITLSSTNR
                     IADQINTSRLMALEGKSYQYQAVVEGEFNANSYPTDPLLVLKEGAQVMFVKNDPAKEF
                     VNGSIGKVTSLEHDRIRVCIEKDAGTRILDLAPLEWQMVRYRLNKEKELEAEVTGLFR
                     QYPIRLAWAITIHKSQGKTFDRIRIDLGRGAFEFGQTYVALSRCRTLEGIQLSRPLMP
                     ADILVDQRVTEFYESIRKIKLAEYKPWTRPGIEGSSLDLGI"
     gene            52186..52923
                     /locus_tag="PKM27_04735"
     CDS             52186..52923
                     /locus_tag="PKM27_04735"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNR06600.1"
                     /translation="MARMIMEETKPFFFKQFKVAQDKVVMKVGTDGVLLGAWIDVTGA
                     ATILDVGTGSGLIALQLAQRLEGKADIHGIDIDPAACEQARENFTASPWGGCMTVIQD
                     SIQDYSGRESKKYELIVSNPPFFTGGLLSLNPDKNDARHTTKLPHGDLLRAVSRLLKP
                     AGRLGLILPYLQGLRFIEISESYSLFPLRITEVRTREDKPVERMLIEFTYDKRVTPQG
                     NTLVIHQGENDFTPQYKDLTRAFYLNF"
     gene            52933..54210
                     /locus_tag="PKM27_04740"
     CDS             52933..54210
                     /locus_tag="PKM27_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR06601.1"
                     /translation="MSFSPIPWIFNSNIYEVNLRQYTTSGSLRAFTGHLRRLRDMGIE
                     ILWFMPLTPISIKARKGTLGSYYAASDFATINPEFGSEQDFRELVDQAHRLGLKVIID
                     WVANHTGWDHTWTRSHPEFYLKNDAGQFFEKNGWEDVIDLDYNSKAMRQAMTEAMAGW
                     VRRFNIDGFRCDMAMLVPLDFWVETRNALDRIKPLVWLAECEDPAYYAAFDTMYGWEW
                     MHATSAVYRGQHDKFHLEHILFKYRDRMPRGRRICLFTSNHDENSWNGTEFERYGDSA
                     LLFAALNIILPLSIPLIYSGQELPLRHRLKFFDKDEIPWSGSPDLADFYDGLLEIRKT
                     NPLYGLPEVALSLNKLCDDPRILSLQAGIPGQRDVAGIFNLSPMEVSSQLNGYHLPGV
                     YKNILTRESFNPASNHPFKLSPWEFRLFENTSV"
     gene            complement(54224..54481)
                     /locus_tag="PKM27_04745"
     CDS             complement(54224..54481)
                     /locus_tag="PKM27_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNR06602.1"
                     /translation="MHITFEELRQIKHSLPTGGVSKIAHDLNIDEQTVRNYFGAKKFV
                     DGKIVDQHIQPGPNGGIVELEDSTILQKAKELISQTHAMSN"
     gene            complement(54626..55783)
                     /gene="ald"
                     /locus_tag="PKM27_04750"
     CDS             complement(54626..55783)
                     /gene="ald"
                     /locus_tag="PKM27_04750"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNR06603.1"
                     /translation="MAPTIQLTKYHYLMIIGVPKEIKSNENRVAVTPAGVMELSKRGH
                     TVQIQSTAGEGSGFSDEDYFKAGAKVLSGIEDTYRSSDMILKVKEPIESEYDLIRAGQ
                     LVFTYFHFASSEALTRAMISNKAVCLAYETVEAPDRSLPLLVPMSEVAGRMAIQQGAK
                     YLERPSQGRGILLGGVPGVPPGKVLILGGGIVGTQAAKMAAGLGAMVTILDVNLNRLR
                     HLADILPPNVVTMYSNEYNIRRLIGNHDLIVGAVLIPGAKAPHLITREMLKSMRPGTV
                     LVDVAVDQGGCIETCRPTTHADPVFIIDHIVHYCVANMPGAVPYTSTIALTNATLPYA
                     LELAGKGWKKACNENPGLRKGLNIVDGKVVYQGVAEAFDLPYQPVEAVLEA"
     gene            complement(55866..>57364)
                     /locus_tag="PKM27_04755"
     CDS             complement(55866..>57364)
                     /locus_tag="PKM27_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06604.1"
                     /translation="PTTGEIKVWAKDLDAGSYDNCTARENLKFSFGPSIADSCKVFKC
                     EDIPNGISFTVQIDIYVWDEAGNFDYCATFISIQDGAGDVCDDAASVAGSISGMVRTQ
                     QNEPVEHVLVEAQSNLPIPAFKTSVSGQYAFVNLPMHSQYILKPTRNDQASNGVSTID
                     LLMIQKHILGVQPFSTGYDMVAADVNNDREITALDLIELRKLVLFLYNDFPANKSWRF
                     VPKSFKFNMAKPYEFPEMIDIKGLSSDELNRDFTGIKIGDLNNTVQAHSLLGMEIRSA
                     VKPLTFQTTDLTLTSGEEATITFTSPNFRDIEAFQFSLKHEGLQILDIQGGSLSIDQQ
                     HIGHIRNNYLTASWDHVSGQTIAAGHPLFTLRVRALKPLRLSDNLAINSMYTRSESFN
                     GKETGEVRLVFGNQENEAHFSLYQNVPNPFQTYTLISFDLPREETITLRVTDLTGKTI
                     HVQKMNGIKGFNQIRLDKGVVGGSGVYYYTIETAAERATRKMILMGTH"
BASE COUNT        13935 a        14427 c        14738 g        14264 t
ORIGIN      
        1 gatggtgcgc tccctgccgg tttccgggtc tggggcatcc tttattcaga ttctattgcc
       61 ttcaatattg gaaacgacgt cagcacgatc agagcggttt cagggtgtgc ccgattatct
      121 gaaaacctga tccccgtttt cagagatgtc cccaggagcc tggacgtcat gtttagcaat
      181 ggccgggtag ataccaacat ctgtggttca aactccaccc tggatacgat ccggctcaaa
      241 acggtaggtt catccgtttc caaacagatt tttgtattga ctttcttcaa tggcctcatt
      301 ttggacacct tttccaccct ttggtttccg atagatcaac tttcgatcgg aaactacaga
      361 gtttatgcca tgacctatac cgggagtgta accattcaga aaaacgtttc caggctgacc
      421 gataagaccc tggccacggg ctgttttaat acggggggtg tgcctgcaag ttttgcggtg
      481 gataccctga cggtcggttc actggtcagc cttaccagcc aatctgtttt ttgcccgaag
      541 gatggcagtc cggatctcta cacggtaggt gtggaggatg gcgagtcacc ctttgtagcc
      601 accctcttgg tggatcagaa tcaggtatgc agacaggtgg ctcctgggct gacgattgac
      661 ctggataaca acccggaagg gacctatgac atcatggttt tgcaatattc agggcaactt
      721 ctggtgaagc cgggggatac ggtatccagg ggaggagtga cgaaattcag ttcggactgt
      781 tatgccctgg ctctgaatga attcgaagta cgcctggttg aaccctttgc agggagcatc
      841 ctggccgaag gccagaaaac cttgctattc gcctgcccgg gggataatct tcctgaccgt
      901 tttactttca atggattcgg ccaaagtcca aacagttacg cggtagtggc cgtcggggca
      961 ggcaataaag tccttgccct gactaccggt ggcgggttta ttgattttga aaggtttcct
     1021 ttaggtcaga gcagggttta cggtatctct tacacgggca cgctgagaat tggtgtggat
     1081 tccctttttg acggggacct gagtaccgac tgttactccg taacctccaa ctttattacc
     1141 gtattgcagg atgttgccaa aggaggcacc gttaggcttg cagatggctc gacaagctat
     1201 tttgtctgtc ccggggataa ccttgcaagg acggtcccac tggttaatac cggtgccagc
     1261 aattcaccct accaatatgt actcacctcg gaaaccggaa cggtgctgga ttttattatc
     1321 aatgatacgc tgaaaattgc ttccagtacc ctgacttcca tgcgtatata tggggtggcc
     1381 tataccggca atatcgtgat cgccaaagcc agtaacctgt ttgtttctgc cttctcagat
     1441 gggtgtttta atgtgtccag caattttata agggtcctcc gcgatgcccc caggggtggg
     1501 ggggtacggc ttctcgacgg gagtaataaa gaattgtttt gtccttctga cacccaatcc
     1561 aggatcctgg aagtgcggaa tgtcagcgta tcccaaaacg catataattt agtgctgacg
     1621 gacagcaatc tggtggtctt gtccatcgac accgccttca aattcaattt tggcggattt
     1681 gcggaaggac agtattatat ttatggagta tctttcatcg gcgaacgatc ccttaaaccg
     1741 ggagatttgc tggacttttc agaattttcc accggctgtt tttccttcag ttccaatttt
     1801 gtcctggtca ccattggtac gatcgacgga ggagtcctca cttcatccga aggttcagac
     1861 cttgcttttg tatgcccggc caacctcgat ctcgatatca tccggatcat tcctcagaat
     1921 gcaaaatccc tgagctacct gtacgtcatc accgatgaca aaaacatcat cgaaggtttt
     1981 agttcatccg atcagatcaa ttttggcagc ggggtgatca ataccaccag ccgggtgtat
     2041 ggggttgcct ataagggggt tttccaggcc acggtcaacc aaaacgcctt gcaggccgtt
     2101 tattccaacc agtgttttga cctgtccgat aatttcgtaa ccatacgcaa gactgttcca
     2161 ccgccgcacc ggatccagtc cagcactctg gataccgtgc tctccttttg taaaaccgat
     2221 gacagggggg atacggtccg gttgtccact tcggattccg tcggattcaa gacggcctat
     2281 atcctgctgg attcaacttc caaaattttg cgaatttcct ctgtccattc catgatcttt
     2341 gatcaggagg ataccggcac ctacaggcta tattcttcga tttacacggg acgactgctg
     2401 gtgactccag gcctcacctt gatccccggc gtttccattt cggacgattg tttttcattg
     2461 tcctccaatt ttctgaccat cagcatggtg aatgagggcc cggcctgtgt gattacttcg
     2521 acgaagcagg aagactggta ttcgggattc aaggtctatc caaatccttc aaaagaagaa
     2581 ataagggtgg agtttaacct tcagaaccat aaagggggtg agggtaggct ggatcttgtc
     2641 gacctggccg gcaggtcagt ctatacgatg cccttgaacc tgcagatcaa gaacaggttc
     2701 attgtgccgc tttcaaaact tggagcggga atttacctgc ttcagatccg gacacaaaat
     2761 cttgtaacca gccgtaaact ggtcatcgga aaataaaaat gtgtatgagg gagcccgctt
     2821 aggcctgggg cgcatctact tcaaccttgg gggccaaggc ctgctttttt tccttaatcc
     2881 gtgctttttt gccggagagc gcacgcaggt aatacaattt ggatcttctt acttttccct
     2941 ttttcagcag ggtgatgtcc actatgctgg gtgaattcag ggggaaaata cgctctaccc
     3001 cgaccccatt ggagatttta cggatgcaga tggtttttgt tcttcccgaa ccagtaattt
     3061 tgatcacgtc ccccttgaaa ttctggatgc gttctttatc cccttcaatg attttataac
     3121 tgatgttcac attgtctcca ggcttaacag tctcactcga acggtctttc agcaactgct
     3181 cctgcacgaa ttgaatcggg ttcatctttt aaattttagg gtgcaaaaat agcactttcg
     3241 gttcatttat tcttgaaaat ttatctctga ccttccttcc accgtgcgga gcaccggtga
     3301 gacacgaaag gtgggaggct cccccggcag ccgcatgtag ccggtccctg cagggaagct
     3361 tgaaaatgag agacttgtaa gattaccaac ggggggcaag ctctcccgga aaagcttact
     3421 gcgggtcgcc ttatctttgt cgaccatgat caatacatcc aggcaataca ggaaggtcct
     3481 catggaggcc aggcagttgt ttattaaaaa aaacaaggat tatggaaccg cctggaggct
     3541 gcttcgtccc gcctccatca ccgatcagat atatatcaaa gcggccagga tccggtcggt
     3601 cgacaccaaa aaagaacaaa aaatcgggga cagcattgaa aacgagttta tcggtatcgt
     3661 caactattgc ctgatggccc tgatgttgct tcggtcgggg gaaggggcat ccccggacca
     3721 cctcacccgg gaaaatgtac tggcttatta tgatgcctgt gccgaggagg ttttccgttt
     3781 gatggaagca aaaaattacg attatgacga agtgtggagg tccatgcgtg tttcttccat
     3841 gaccgacctg atcctgatga aaatcgttcg cataaaggaa atagaggacc gggaagcgga
     3901 cagcctcgtt tccgaaggag tggatgccaa ttacatggac atcatgaatt acgctatttt
     3961 tgccctcatt ctgcttcatg aatcccatac gcctaacttt taagccatga ccttaagtac
     4021 actgatcatt gccttcgcgg ttgccggggg gctgctgacc ttcctgatca gccagaaagt
     4081 aaaacagccc aaatcctggc tgatttcttt ccttcagaat ttctgcgggg tctggtttat
     4141 tttttccgga atggtgaaag cggtggatcc gatgggcacg gcttttaaga tggaacagta
     4201 ttttgcggcc tttcagcaaa cctttgaacc cacctggatg ggttttattg ccccggtgtt
     4261 cccctggatg gcccaatcgg ccgtaacgat ttccatcctg atgatcatcc tggagatcct
     4321 ggtcggcgtg gccctcatca tcggtttcaa acccaaactg acggcctggg tgtttttcct
     4381 tctcctcctg ttctttaccc tgctcacggg cttcaccttt ctgacgggtt atgtaccttc
     4441 gaatgcaaac tttttcgaat tcaaagcctg gggtgatttc gaacccctgc aaatgagggt
     4501 ttcggactgc ggctgtttcg gcgattttct taaactggat cccaaggttt ctttcctgaa
     4561 ggacctcggc ctcttgctgc cggccatcgt ttttctggtc tggacgcggc tggaacatca
     4621 gttgtggacc cccttcaccc gattcctgtt tattgtactc gcaggactcg gttcctgggg
     4681 attttgccat tataacacct atgtgaatga gcccctttgg gatttccggg ctttccgggt
     4741 cggatcggat atcgcctcca cccgcaaggc ggaactggaa gccgcccaac aggtccagat
     4801 tgaatatgcc gaattaaaga acaaacaaac caaagcggtt acccgtatct cctatgggga
     4861 ttacatgaaa gaattcaaaa aatatcccaa ggaggactgg gacaccaaat ttgtttatgc
     4921 ggaatcgaag gtccccaaga ccaaggtcag tgattacatc atattggata ccagcggttc
     4981 agacattaca caggccttgc ttgatgaaaa aggatattcg ctcatgatca gcgcctacaa
     5041 agtgcatgga caggtacagg aagtgaagac cagcgtttca gatacggctt tttcggacag
     5101 cctggtgatc acgggtacgg ataccctcag gttccgggtc atcaaagagg tggtccaccg
     5161 cgaatccacg ctttcgagtt tcacggccaa tgcggaagaa gcggcgcttt tcagggacca
     5221 gatcaatccc ttggtacacg ggttcctgga caagggagtg aaggtgtttg cggtcaccgg
     5281 aggtcttggc aactcgcaga tgcgggattt tgccgctcaa accggcgccc ggtatcccat
     5341 ttatcaggcg gacgatatcc tgctgaaaac gatcatgcgc tccaatccgg gcctgattct
     5401 ctggaaggag ggtaaactga tcgacaaatg gcactaccgt cacctgcctt cggctgaagc
     5461 cctggaaaga aaaatgaaat aacaaacccc cacggcctgc ccgccgaccg aaaaatgcgc
     5521 ggtctttttc atggaattca cattcaatat ttaactatat ttgtgcgttc agaagtgacg
     5581 acatcaaccc atgcttagta ggagaaattt acgtgtaaaa gtgatgcaaa tcctgtttgc
     5641 cagaaaccgg gatgcattta tccaggaagc ccatcttccg aaaatgttag atcagtccct
     5701 gcggcaatcc tttgatttat accttttcca gttatacctg atccagaaaa tctgtgaaca
     5761 ggccaaaaat gtagcggata aaatacagaa caaatacctg cccactacgg aggataaaaa
     5821 ctttgaggcc gtcctttatg agaacccgct gatccagaac ctggtgcaga ataaaaaact
     5881 tcagaaactt tttgtcaagg gtgaatttga caaatgggtg gaagaagacg tttttaaaaa
     5941 aatctacagc gagttcgcca aactcgattg gtataaggct tattccgata aatccctcgc
     6001 cgagaagaat cacctcgaaa ccttgctgga actctaccgt tttttgcgga aaaacgaatt
     6061 attcaatgaa atcgttgaag atcagttgta caactgggaa gacgatgatt cgctgatcat
     6121 cggcgccatc aaaaaaaccc ttaaggccct tccggcatcc gaagaattct ataaggaatt
     6181 tatggtcacg gaggaagaaa ccctgctctt tggcaagacc cttctgaaaa aggtcattga
     6241 gcaagaggat gaacttgcag cgatcatcaa acccaccctt aaaaactggg attccgaacg
     6301 ggtggcgatc atcgacatga tcctgctgaa aatggccgtc tgcgaattcc tgtatttcaa
     6361 cacgattccg accaaggcca ccatcaatga gtatgtggag ctttccaaac tttattccac
     6421 ccctaagagc aaagacttta tcaacggggt gcttgaccgg atcatgaaag acctgaccga
     6481 atccaaccgt atccaaaaag cgggcagggg gctgctagca gaataatatg catgagcagg
     6541 tattgatcct ggattttggt tcccaataca cccaattgat cgccaggcgc atccgggaac
     6601 tgaatatctt ttgtgaaatc aagccctatc accagtacca ggccggcgac cacacccgtg
     6661 cggtgatctt atcgggcagt cctttttccg tgagggatgc caatgccctg cggatccccc
     6721 tcgaccttct attgggtaag gtgcccgtcc tggcggtttg ttacggagcg caactcctgg
     6781 ccgactgcct cggaggaagc gtggtcaagt cggaccaccg tgaatatggc aaagcctcac
     6841 tgcaaatcct ggaccttaac gatccctttt tcaccggggt agaagatggg tcacaggtat
     6901 ggatgtccca tgcggatacc gtgcaagacc tccctccgca ttttaaactg ctggcctcca
     6961 cggattccat tcgcgtagcg gcattcaagt cagatcacta cggagcgcct gtttatgcca
     7021 ttcagtatca tccggaggtc acccattccc tccaggggaa acggatgctg gaaaatttcc
     7081 tgtttaatat cagtgggatg aagggggatt ggacggccaa tgccttcatt gaggaacagg
     7141 tggggatgat ccgcaggacg gtgggagaag atcatgtact catggccatc tccggaggcg
     7201 tcgacagcac cgtggcggcg gcgctcatac accgcgccat cggccccagg ctccatggat
     7261 tttacattga ccatggtctg atgcgggaag gagaatccga aaaggtgatg acgctttatc
     7321 accaacatgg gtataatgtg aaaagggtgg atgcgtccgg atatttttta aaggcgctcc
     7381 atggggtcag cgacccggag acaaaacgta aaatcatcgg gaagtctttc atagacgttt
     7441 ttgaagagga agccagggct ctgagcgggg tgaaatggct tgcccaaggc accatctatc
     7501 cggatgtcat tgaatccatt tccgttcacg ggccggcggt aaccatcaaa tcgcaccata
     7561 atgtgggcgg ccttcctgaa aaactccatc tcaaggtgct cgaaccgctt cgaatgttgt
     7621 ttaaagacga ggtgcgacgg atcggggccg agctcggaat tccccaggcc atcctgggcc
     7681 gtcatccatt cccgggcccc ggactggcca ttcgtatcct cggggacatt accccggaga
     7741 aaatcggctt gttgcagaaa gccgatgaaa tctacattac cttgcttaaa aaatacaatc
     7801 tttatgattc gatttggcag gccggggcca tcctgctccc ggtgcggtct gtaggggtaa
     7861 tgggagatga acgcacctat gaatataccc tggccctgag ggcagtgaat tcaatggacg
     7921 gcatgacggc agaatggagc aggcttccct atgattttct ggccgaagcg tccaatgaaa
     7981 taataaataa agtaaaaggg ataaacagag ttgtttatga tatcagcacc aaaccgccgg
     8041 ccaccattga atgggaataa gctgctcctg ctggtcagcg cggtgctatg gactctcatt
     8101 tccagttgtt cggccgccaa aaagtccacg gcagcgccca aagaacccgt tcccgcggaa
     8161 atcgccaaag aggtttataa tcccaggagc gggaggtatg agccggccac tccctccaat
     8221 acccgggtcg atacggttca ttggacacgg cctgccgaag cctggattac cgatgaagat
     8281 ttaatgaccg ttggggccga cgaagcttcg gtcaaatcag cctaccggat cgccgtgctg
     8341 cttcccctct ccgacgatga tttgaataaa gacctgaacg ccgaggggtc cgataaattt
     8401 tttcactatt acgcagggat gaaaatgggt atggaggata tggccatcca atatcccaat
     8461 atccgggttg atgtgctgga tgtcaacgcc aatccttatg ccatgcagcc cattctccaa
     8521 tccgctgcgc tgcagcaggc cgatctcctg gtcggcccac tccggaggga ccatctcaat
     8581 gaggccgccc ggttttcttt gcagaaggat attcccatag cggcgccctg gaacgcattc
     8641 aggaccatag aaaacatcaa tccaaactac atcctgctcc gttcttcgct cccgacacat
     8701 tgcgaggcgc tcacccggta cctgcttgaa aacttcaatg gcgaagaaat ctgccttttg
     8761 ggaagggaaa gattcaaacc cctgatgaat tattttcaat cctttttgac tgaattcaag
     8821 gggccccttc aaaagccgtt cagggaaatc ctgatcaagg atgacttccg ttttggggat
     8881 gattaccaat acctggacag caccaagaaa gtatatatcg tcacggaata tgaagacccc
     8941 aatgtggttt ttaacctgct tcggcatatc aacctgatga gaaacaaccg gcccgtcacg
     9001 gtgatcggaa tgccttcctg gcaagattac ggtactgatt ttattaacct tttttcacaa
     9061 ctgggagtga ctatcagcgc ttcctcccat cccgattaca gccaggaaaa ggtgaacgaa
     9121 ttcaaaaagt tatttttccg gcgttatcag acctttccgc tgaaggaagc atttgagggt
     9181 tatgatgcca ttcaatacct ggtccagaga cttgccagta gcgggcgcaa atttcatctg
     9241 caagaagacc ttcagactta tgatggcctg gcgagttctt tccgcctgga aaaagtgatc
     9301 aatacccagg agccggtgga tgaccgtttg agaaatgtca tttgtgtcga aaacaaagcc
     9361 cttcatatcc tccggttcga agagttcagg tttaatatta tacgatgaat ggccacctgc
     9421 tggaaaggcg tgctcagaag atccgcaacc tgatcgcaaa acggcagtcc catttcacgg
     9481 tgatcctcga aaatgtccgg gaccctcaca atatcggggc ggtcctgcgc acctgcgacg
     9541 cggtgggcat ccgggaggta ttcctggttt attccgacca acgggaaaaa gaccggtttg
     9601 tgctggggaa aagggcctca tccggagcgc gcaaatgggt ggatgtctat gagtttgatg
     9661 ccataaaaac cgcagtggat acccttcggg acaaaggcta tgaacagatt tgggccaccc
     9721 ggattgtgga ggggagtaaa agcctgtttg aactggacct cagccggaag gtggctttat
     9781 tgttcggcaa tgaacatctt ggagtgagcg atgaagccgc cgccctggtc gacggaaatt
     9841 ttgtgattcc ccaggtagga atggtacaaa gccttaatat ttcagtggcc tgcgccgtca
     9901 gcctttacga aggttacagg cagcgcaaac tgaaaggctt ttatgacgac tctccaacgc
     9961 tttccccgga cgaacagcag aagatgtttg aatattactc ctctagggaa ttggttcgtt
    10021 acaagggaaa gacggccatc cgcccccctg aaatcaagcc ttaaacagga agatcgatgc
    10081 agtcgccctc tcgcgccagg ctgacggttg tattccttgc ttcgtcctta tgcctggcca
    10141 gcatttcttc aagtttgagg tcggaatgca tggggtcatg atggaaaaga tataattgcc
    10201 ggaccttgca caactcggca aactttatgg cctggtcgta ggtgctgtgt ccccatcccg
    10261 ttttagccgg gtattgctcc gtatcatact gggcatcgtg gatgagcaca tcacaacctg
    10321 cagcgagctc atatcccgat gtccattcgg cggatttcgg gaaggccggg acacccaggg
    10381 cgacttcatg atcgggaata taacaaaagc tatgagtgtc atcgcttatc cggaacccaa
    10441 gggttggccc gggatgacag acaaaggacg cgttgatcga caagtggctg gttcgaagac
    10501 ttcgcctggg cagggcgtgg aactggagat ctgccttaaa atcccttatc cgcacaggga
    10561 aaagaggagg ggagaggtat ttggtcagta ttttgaagag ttcttcggaa ttggatgcag
    10621 gcccataaat attaacccgg cattcgctgt tgaaaaaggg ggtgaaaaat cccaatccct
    10681 ggatatggtc aaaatgcata tgggtgatca ggatatggaa ttcccggatg gattttacca
    10741 gttcgctgtc ggcaaggggt ttgattcccg agccggcgtc cagaatgatc aactcccctt
    10801 cgttttccac ggacaggcaa ctggtattgc cgccgtatac ctgggtatcg ctgttgaagg
    10861 tgggcagcga gcctcgggtt ccccagaatc gcagcctcat agcctgttga cgaggtttat
    10921 aaaaataagc gctcccaatt ccctctggga aaggccgacg agggggacgc atacgatcat
    10981 gagtttctga ttgatctcct cgaaatttct catatagtat tccccctgga cgaggtggcg
    11041 tacgcttaat actttaaaga agggaatttc atgtaaatcc gtgctttccg tgaaaccctc
    11101 gtggttgaag aaccgaccgc cccaatcccg aaggtcaatt tccccgtctt catcaaaatg
    11161 tttacccagg atgaatccgg cggcttcatt ataataaacc attttaccat catggtctat
    11221 cagggccatg ggtacggcca ggtattccga cagttgtcgg gataaaataa gttcaatatc
    11281 ctgggaagac atatgtggcg cagtttggtg cgcccaattt aatgaaaaaa ggatagatgc
    11341 tgaaagtttg aagccggcag gctaaaccag gctggacaac gatttcagaa ggacgtttac
    11401 gctgtcggat tcgtggcctg ggtacagctt cttgaatttc tgaaagcagt ctttggcgga
    11461 tttgagttct tccatctcga ggaaggcgag gcccaggttg tagtagacat cggcaaattc
    11521 agggtcaata agggtgacca cttcgtaatg aagcaccgcg agtcccgtat tgccggccac
    11581 gaagtataca ttggccaggt taaaatgtga ttcacaatga cggggttctt ccactagggc
    11641 cctgctgaaa taatcaatgg cctgggccat tttctggcgg tgggcggcga tcacgcccag
    11701 gttgcaatag gaatgggcaa tatgctcctt tttctcaatg gatttcaggt agtattgttc
    11761 ggcagaaggg tcgttgcgca ggtcaagttt cagcgcgagt tcgaatgtat tttcggcgag
    11821 ggggagctga agaggctgga cttgggaaaa caaatcaagc tggccctctt ggcccggatt
    11881 acccgcgtgg tcatccgccc gcttgaaatc tatgggctga aaccgcttcg cctgataggg
    11941 gatcactttc gccatggatg gtaaagatag gcgatttggg aatgatggaa tggtctgaat
    12001 tgaccctcat cacagggctg gggaaaccgg aaatggtttt caagctcccc tggatcaggc
    12061 ggatttcttt ttgcttgtct tagccgcttg ttttcctgcc gttccggtgg ccttcaccat
    12121 gggtttaatt tcggacttgg cctggctgat gctttctttg agcgctgtca tgatgtcgat
    12181 gaccggggcc tcttcttctt ttactgaaac aatttccttg ccggcaattt ttgcctggat
    12241 tatttccatg agggactcat aataacggtc tttcaattcc agttgggaaa aggatttggt
    12301 catggtttcc acgaggacat tggccagttt tagctgggct tcatcgattt tggcatgaag
    12361 gtccagttgg ggtatgtttt caatgctcct cagttcgtgc ggatagcgca acatatacat
    12421 catcaggccc gggccgctgg gggcgatcag gaccacctct tccttttccc tcaggaccac
    12481 cctgcccacc gccaggcggt tggtctggtc gagacaagac ttgagcaggc cgtatgcttt
    12541 ggtggctact tcgccatcgg gacctgcaaa gtacggggtg tcaaacaagg ccggatgcac
    12601 ttcgtctttg cttacaaagg cctcaatatc aattattttt gtacttttta gacggacttt
    12661 ctgaaggtcc tccggttcca tgatcacaaa ctggtccgag gcatattcat accctttcac
    12721 gatatcctcg cttttgacgg attcaaggca ggttttacat ttcttatcat acccaatagg
    12781 gcccatgtct tttttatgca actgcctgaa actgatggac tggctggttt ctatcgcatt
    12841 gtataaacgg atggggatgg tgaccagcga aaagcggata tggcctttcc agattgacct
    12901 catgttttca aagttaagcg ggttaaaccg acggatctgc aaaaattatt ccatccttat
    12961 tcaaggtctg gcttcagctg gataaataca ggttcgcgaa gggtcttgtt ggacgtgatg
    13021 gaagcgtatt ggatttcaca gatatgttcc ggctctaccc aggtcgtatt tttttcctcc
    13081 tccatcgtca ggccaaccgg ttttttgtgg accctggagg gttccaggat ctcttttcga
    13141 atcgttttca tcagggctga atcaaatccg gtgcctacgc gtccccggta aacccagctt
    13201 ccgtcttcgt tcctggatag gagttgaagg gcgccgaata agggttcccg gtctcctttt
    13261 ccggccgtaa acccgacaat ataacaatcc atggtattcc tgaatttgat cttgatccag
    13321 tccgtgcttc gccttccggg agtatagaga ctgtcctttt ttttagccat gatgccctcc
    13381 agccccagcc gctccgctgc catttttaat tgacgcccgt ccgcaaatac gtcactgatc
    13441 cggtaagtgt tttttacctt gagaatgtcc ttgagccatt cctggcgccg cagcaagggt
    13501 tctttgagca ggggccggcc atcgaggtac atgacatcaa agagatagca gcatgctggt
    13561 ttttgtttgc gtagggcttc gatcgccatt tgaccggcgt gcattcttga aatcacatcc
    13621 ttgaacaccg gcttgccctg gccgtcgagg caaacgattt ctgcatccag tacggctaca
    13681 ttcgcattga gatgctccct tgcctgactg atttcaggga attgtgcggt gatatcccgt
    13741 cccgaacgcg agaatatttt aagcccgtct tcctggatct gcaagccgac ccggattcca
    13801 tcccatttta tttcatactg aaattggtgc tctgcgaccg gcagatcttg tgcagcactg
    13861 gccagcatcg gggggatata agtattccgg tcccattgtg cacgatctac ccgctccagc
    13921 agccattcct tttccttaag gttgtgcatt ctgaattctc cttcaagttc cgggctgtag
    13981 aggcgaaaat aaaatccatc ttttttctgt ttggtgatct caaaccggcc gcgggcaaaa
    14041 atccacatgg cccctccgcc gtattctcct ttgggtatga tcccttcgaa ggtcagatat
    14101 tgcacgggat gtggttcggt ttccaccgcc atgcgtttga tgcccggctg atccggcatg
    14161 cctctgggaa cggcccaaca ccggaggacc ccatccaatt gcagccgcag gtcatagtgc
    14221 agccgggagg catggtggcg gtggatgaca aaggcgtttg gggaggtgta ttcatcagca
    14281 tccgatacag ccggttcggg gcttttggca aagtcccttt tttgtttgta cctggcgagc
    14341 tgctcggggc tcttgtgttt tgaaccttcc gggttatggg ccctgggctt agcctggacc
    14401 ggccggaaag gatgcagctt cacggcaagg ctgtaaaatc catcccaggc atccccatgc
    14461 ctggagattt gttccatggc attgtggatg gaccaggctt ttgcactttc aagttggtcc
    14521 agggcgttcc aatccacggg catggatacc ggcgctcccg gcttacccct gagtgaataa
    14581 ggagctatga tggtctgtga ggcgtggttt cgctggatat cgataaacag cttatccgtt
    14641 ctctggttct ttcggatgtt gagagtgcat aaatcagggt ggagggcagc aaattcccgg
    14701 gtgaggtttt taagcacctc gatgaccact tcataaggat gatcgggttc tatgggaaca
    14761 aagagatgca ggcccttgtt gccgctggtc tttacaaatg cggtataatc ataccgcgat
    14821 agaaattgcc tgagtgccag ggctattcct ttggttttat cccaccatcc ctcttccggg
    14881 gggtccagat cgaaaaccag gaggtccggc ttattgtatt ccggtttctt gcaggtggcc
    14941 atgtgaagtt ccaatccggc caggtttgcg atccatacca gggtggcttc ttcttcggca
    15001 agaaggtagt ttttcttctc cttgcccaat tcaatactgt cgatccattc cggtgtccag
    15061 tccggcctgt ttttctggta aaacgcttcc ccttcgatgc catccggata gcggatcagg
    15121 ctcagtggcc tcccccggat gtatttgagc agggtcgggg ccacctggag gtaatattgg
    15181 atgacttccg ccttaaggat gccttcctgt ttgtagatgg ctttttccag gttgctgagc
    15241 ttcaggatcc ttttccctac ccgggtattg tggaaggtgg attttgccat aaaaaggaaa
    15301 caaattaaaa aaaagtttaa tatgataaaa aactttatta aataaagaaa atcaatagtt
    15361 tatattttat aaaatattat catattataa atttttataa tttatggaac atttcttgct
    15421 gccaaggatt cggaaggtaa gccaaatttt aaataacctg taatcccaat ccgtacatgt
    15481 acacttcaat tccggcaagg agatatcaga caaggatcat cgcagctaat aacttagcca
    15541 gggtttttcc ggttttgatt ttaatttttt tcctgaccgg gctgctccgg gctcaaagcg
    15601 cctgcccgct gaactgtaat gaccagatca atatttccct ggatgaaaat tgtcaaagga
    15661 tcctggtgcc ggacgatgtg ttggaaaacc cggacagttg ccagaacgcg gtggtttcac
    15721 tgacttaccc ttacggcacg cattcgtatt cgtctccctt ggtcgaccgg acgcatctgg
    15781 ggtacaccat tatttaccgg gttacggata cggtaaaccg gaattcctgc tggggttata
    15841 tcctggtgga ggataaattt cctccgcaac cggtatgtaa aagtattgaa atcagttgtt
    15901 tccagcttgc ccgcttcaac gacatcctgg gtaaggtcca ggacaattgt tcccaggtgg
    15961 gtaaggcggt catcagcgcc ctgtattggg tagaatacgg ttgtgattcg gcgtttcttg
    16021 gccgggtctt ccggaccatc cgtacctatg accagtgggg caattcatcc ctttgtatgg
    16081 acacccttcg catccgcaag gacagtttac atcatatcaa atgtccggac caccttcagc
    16141 tcaactgccg catcaactgt gaaaaaccgg gaggcggccc tgaactgatc caattttctc
    16201 cagaccccgg tcatcccttc tatccaagcc ccgaattcct gcttcaactt caggaaagag
    16261 atactttcgg cagcaatacc aggacctgca tcccgcggga ttccatgatt gtgccgggca
    16321 ttcgggacat tctcattgtc gcggttctca agaatacccc tttccctgcc cattcaggac
    16381 ttagcggcct ggcctccagc gatacctgtg tagccctaga cacctgtgta aacatgtgga
    16441 ccaccgaagg cagccatagg ggaggccttt gcaagatcag cctgggatat aaagaccata
    16501 tcgtaccggt ctgcggcagc ggttttaaga tcaggaggga atggaaaata gcggactggt
    16561 gtgctcagcg ggatacagta tgtgtgcaat acatcaaaat tgaggaccag gaggcaccgg
    16621 tcatcaccgg gggcaggacc cgctattaca gcagtgtaaa ggcgcatgat tgcttcgttc
    16681 caataccgct ggaagcctta caagctgaag attgtgaccc cgtcctccaa acctattacc
    16741 tggaatatgc cgatggcgac aaaggcaaaa aaatagtatt gaacggtacc ttgcccgcca
    16801 gtcttaattt gacggcaagg ggagggatta acggcaaacg gtgttttgaa atcaggctcg
    16861 ctctgacgga cccctgcctc aataagaccg aaaccctgct cgaagcctgt atctccgatc
    16921 tcagcccccc aaccccattg tgcgatgaaa gcgcccaggt caccgtggac ccggccactt
    16981 gctgggccag ggtttatgcg aaggacctgg acaacggcag cagggacaat tgctgcaacc
    17041 tccttcattt tgccgtggcc ccaatgagtc aggtcgaaag cacccgggcc caggccatct
    17101 ctaaaattga aacggtctgc ggaagaaatg aatactgggg caagaaggcc ttttatgatg
    17161 cgtatatcga aaactggatc aactgttatg tattcaatga ttacgtcgat ctcactgaat
    17221 gcgggaatgt gcaactcgtg ctccgggtgt atgaagcctg cggcataccc ctgtacgacc
    17281 cgcacacctt tccctgttcc cctcacgatt ggttttgtta caataccagc cacctgttca
    17341 gggcagaatt taacttcaac tggtttgaca agcacgacaa atggttcaaa gacctcacgc
    17401 ccaggaattg cgattgggcc ccaccggttt tttgcgggga ttcgctgtac gcctggctga
    17461 ctcacctcaa aatcagggat cacgacaaaa aagaaggatt tccaggctcc attgaatttg
    17521 aagatttccg gcatttccgg catatctttt atctgagcaa aaattgcctg gaactggact
    17581 ttacacccga ttatgtgaat aaaatgaaag ccctcacggt ttttgaaccg ggcaatacct
    17641 gttcgaaaaa cctgtatcac gattgtatgg tccgcgtcgc ggttgcggac aaaaccccgc
    17701 cggtctgtga aaaaccgaaa gacctcacgg tttattgcga cgggcagtcc ggtaactata
    17761 cctatgccca ggccaaatac tggagctgca gtgacgggaa ggaaaaaggg aaatggcctt
    17821 atgccattga atgccgcaaa gaaggggatg gggacctttc cgatgccacc gatcccacgg
    17881 gtaaaccatt cggatattac ggcggaccca ttttggtgta taatcatgac gatcatggcc
    17941 cttccccggc ggattgcgaa gagccgtaca acacctggaa acccgtttat tgcaaacaat
    18001 ggttgtgcct ggacagcctg gataccggcc gtaaaaccga ctataaatcc ttcttttcca
    18061 aagccatctt caagaacggc ggaaaaccgg cggagccggc gggttccaat gctttttata
    18121 tctgggacaa ttgccagctg gacaccgcgg tgaaaatcga agataccgaa tacctcgacc
    18181 catgcggcgc cggatggctc aaaaggacct ggacggtaac ggaccgctgc ggcaataaaa
    18241 ccatctgtga tcagaagatc aatgccgtcc accgctcaga cttcgaagtt ctgtttcccc
    18301 ccgatgtcgt gatttcctgc atggattccg cgagccttta tcccgggctg gatcaaatca
    18361 cagggcaggt gatgatcatg gatgacgaat gtgagttggt cggggtcaac tatgaagaca
    18421 cccgctatga tatcgtggtg gacggttgtt ataaaatcgt gcggaagtgg acgctcatcg
    18481 actggtgtaa atacgatccc ttgcaaaagg aacgcgcacc ggatattatc gtaaacgaca
    18541 gcctcgtagc ggataaaaat aaccgcgcat gcgtgatccg gaacctcaaa gacgacgggg
    18601 atgggtatat ggtctacacc cagatcatta aagtggtgga cgagttagcc ccggtactga
    18661 cctgcaaaga cagcctgctc tgcacttatg tcgacgggtg caaggaaaga ttcagtttca
    18721 gcttctccgc tacggacaat tgtaccccat ccggccagat cggctacaaa tgggaactgg
    18781 atgcaggagg cgatgggact attgagaaaa ggagcatccc gaatgtaaaa aatttcagtg
    18841 aagagctggc cagcggccaa taccggctta cggtttatgc aacggaccat tgcggcaatt
    18901 acgatacctg cgtgatcaat ctgacagtca aggattgcaa acagcctacc ccttattgtt
    18961 tcaacgggat atccaccgtc gtgatgccgc ggaccggaga aatcacgctc tgggccagtg
    19021 actttaacgc gggcagtttc gacaattgca cggccaaaga aaatctgaaa ttttattttg
    19081 gcaatgagga ttgtaccgga ccgggtgaac ccagtaaaac gtttacctgc aaggaattgg
    19141 gtttccagtt gctttgcatc tgggtggttg atgaagcggg caacggggat tattgcacca
    19201 cctatgtgga aatccaggac cacagcaatc ccagggtctg ttccgtggct tccctccttg
    19261 gcctgggggg aaatgttaaa acggaaaatg cggaaccggt tcaggatgtt caggtggagc
    19321 tcaagtcccc cagcggcctg cccttccaga tgatcaccgg gaacgatgga agatatgcct
    19381 tcgaagggct tcagtttaat aaaaattacc aggtggttcc cgcgcggacc ggggattgga
    19441 tgaatggggt gtccaccctg gacctggtga tcatccagca acacgtcctg ggggtcaaac
    19501 cactccaatc catgtacaag ctgattgccg ccgacatcga ccattcgtcg gaagtcaatg
    19561 tcatagacct tgtggaatta cgcaaactca tcctgggcat atatgataaa ctgccggcca
    19621 acccctcctg gcgtttcgcc gagaaatcca gtaccgacaa ccgttatcct tatgcgatcc
    19681 gggaaatcat tgaaatgaaa ggcttgctcc aatcgggttt ccagcacgat tttgtcggca
    19741 tcaaaatagg agatgtcaac cagtcggtga ccgcccacaa tttattgggc accgaatcca
    19801 ggaacaatga aaaggaatgg gtcctggccg tggatgacct ggtcctggaa ccggggcaaa
    19861 gctatgaagt acccttcagg aatgccatcg gcagtttact gctgggttat cagtttacat
    19921 ttggattgga cccgcaggcg ctggaatttg atggatacca gagcggaagt ctccggctgg
    19981 atccttccaa tttcgggttc aatcacctgg accagggctg gataagcacc agttggaata
    20041 ccgaacctgt tttatccttg caaaaggacg aaaccttgtt cacgatccgc ctcaaagcca
    20101 aaaagaaggt gcgcttgtcg gaagtgctgt ccattcattc taaactgacc ccggccgaag
    20161 cctattccag caccgatgaa accaaggccg tccggcttat gtttaccggc agtaagcagg
    20221 tcacgtccaa aatggtcctg caccagaata ccccgaatcc ttttaaactt tataccctga
    20281 ttggcctgga acttccacag gccggctcag cagtgctgac catcaccgac atttcaggta
    20341 aaaccctgaa agcgatccgt ggtgattttg gcaaaggtta tcaccaaatc agggttgaga
    20401 aagcggactt gcccgcaagc ggggtcctgt attacagact ggcctttggg gatcaggtat
    20461 tggttaagaa aatggtggtt ttggaatgag gcatttttgg ccggctgccc gtttttgatg
    20521 cccaatacca ggggaaaata tccggttaaa ccttgatctg gataaatttg gcctgtatat
    20581 gaaaatcggc ctgctttccg atacgcattc gtttctcgac ccgcacatac ttactgtttt
    20641 cgagcattgt gatgaaatct ggcatgccgg cgattttggt tcacaggagg tgatcgaccg
    20701 tctccgtgaa ttcaagccat tgaagggggt gtacggaaac atcgatgcgc ccgccatccg
    20761 ccaccagttt ccggaagtgt tgcgctggaa ggtgggtgga ttccggattt ttatgacgca
    20821 tattgcgggt tatcccggaa aatacagctc aaaaatactg aaggcacttt cttcggagcc
    20881 ttgtgatctc ctcctttgcg ggcattccca catcctgaaa gtgatgaagg atcagcggct
    20941 cggacattac catatcaatc cgggcgcctg cggacatgaa ggatttcacc tggtccggac
    21001 ggtcatgagg tttgagataa aggaagaccg gctcctgcag cccgaaatca ttgaactggg
    21061 tcaaagggga agattgccca taagcccttg aagattattt attccgggaa gccgggcggg
    21121 ctccctgtgc gggcgtattt ttgcagccat gcaagaccag ggtgatgaac cgatgatgct
    21181 cacggacagc gatgacctgc aggatcagct gatcattgag gtggataaaa accaatcgcc
    21241 ccttcggatc gattcttttt taaataaccg gataaaaaat gtatcgagaa cccgtatcca
    21301 gcaggggatc cgtgcggggg ccatcaccgt tgacggcaaa aacatcaaac ctaattttaa
    21361 aattgccccg cttcaaacca tccgggtgat tgtgccgagg cacgaagcga tcgatattct
    21421 gccggaagac ctggacctgg atgtggtgta cgaggatgac tttttgctcg tggtcaataa
    21481 gccggcaggg atggtggtcc accccggatt gggaaacccg agacataccc tcctcaatgc
    21541 ggtcgccttc cacctgaata ttgatccgcg ggatcctcct gaagcttcgc aggaccttga
    21601 gcgaatgggt ctggtccacc ggatcgataa ggagacctcg ggtcttctgg ttatagccaa
    21661 aacggaatat gccctgtccc acctggccaa acaattttat gatcacactt cctccaggga
    21721 ataccttgcc ctggtttggg gcgaaccggt gccggacaag ggtactgtgg acctccccat
    21781 tgggcggcat cccaggttca ggcagttgtt ttcagccttt cccgaacgcg atcagggaaa
    21841 acacgcggtc acccattatg aggtggttga aaaattgtat tacgtgtccc tgatccgctg
    21901 caggctggag accgggcgga cccaccagat ccgggtgcat atgcaatatc tggggcatcc
    21961 cgtcttccac gatgaaagat acggagggga ccgcatcgtg aagggtaccg tgcataccaa
    22021 gtacaaacaa tttgtggaca atctcttcaa ggattttcca cagcacgccc tgcatgccaa
    22081 atcgctgggc tttgtgcacc cggaatccgg aaaatggatg caatttgatt ccgaacttcc
    22141 ggtggaattc gaagtgatcc tgaataaatg gagaaaatat accagtcaaa aaaaattaac
    22201 cggccatgga cctgaatgaa cgaatccggt tgatggcctc cctgggaagg atgctgcggg
    22261 aatatccgga aagccttcgg gacgccatcc gggaagcctc tcagcaaaac aaatggtttg
    22321 acgaaatgaa tatccggctg gccctgtcca atatcgcgga tcattacctg gatgaaaaaa
    22381 aactttcggc ctggattcaa tcttatgctt taaatgaacc ggtccctaaa accgtgggca
    22441 tcgtgatggc gggcaacctg cccctggtgg gattccatga tttgctttgc gtctttatca
    22501 cgggccatcg ttccctggtg aaactttcgc aaaaggacca ggtcttatgg cccgcgatcc
    22561 gggaactgct gatccgggaa gatcaggcgg ctgcggatta ttttatgatt gcggaccgtc
    22621 ttaaaggcct ggatgccgtc attgcgaccg gcagcgataa ttccgcccgg tatttccatt
    22681 attattttga ccgctatccg cacatcatcc gcaaaaacag ggtgggggta gccgtgatct
    22741 ttggtgggga atcccaggac atactggccg ccatcggcag ggatgtattc agttattatg
    22801 gtctgggttg cagaaatgta tccagccttt tagtacctgc cggttatgat ttttcttcct
    22861 tgctgaaaag ctgggaaccg ttttcagaac tcctggacca tcatgcctac cgcaataatt
    22921 tcgattacaa caggaccctc tttatcatga ataatatacc cttcctggaa agccggtcag
    22981 cgcttttgct cgagcatccc gacctggtgt cccggatcgc ctgtctacac taccaatact
    23041 acgattcccc ggaacaggtc gccggcaagt tgaatgccgt aaaagataaa atccaggtgg
    23101 ttgtttccgc tcagcccatt ccgggcttgg ccaccatcct gcccggagaa gcccaatcgc
    23161 cccggctgga agattatgcg gatgaagtgg acaccataaa atttcttgcc cgattatgac
    23221 gcgcagtgaa aaagcaaagg ccatcctgca aatcctcgat gattattttc ccgaggtcaa
    23281 agtgccgctg agccaccggg atccgtacac cctgttgatc gccgtccttt tgtctgcccg
    23341 ctgtaccgat gaaagggtaa acaaaaccac tcctgaactt tttgccctgg ccgacaatcc
    23401 ggcagacatg atgaaagtcc cggtcgagaa aatcaaacag atcatcaaac cctgcggact
    23461 cagcccggcc aagtccaagg ccattgctgc tacttcgaga atactcgtgg agaaatatca
    23521 ggggaaagta ccggaggata tggcggccct cgaagcgctc cctggcgtcg gacacaagac
    23581 ggcctccgtg gtattgtgcc aggctttcaa tatccctgcc tttcccgtgg atacccatat
    23641 ccacaggctt gcttaccgtt gggggctcag cgacggttcc agtgtggaac aaacggaaaa
    23701 ggacctgaag tccctgattc cacggagcaa gtggatcaaa gcgcaccttc agatcatcct
    23761 ttttggaagg aaatattgtc cggcccgggc ccacgaggcc tccaaatgcc cggtttgttc
    23821 acggtatggg gtgaaaaaca ggatgtaaat accgttcgga ccggtcagaa cggtaaaccc
    23881 agggaaaggt tgtactgcag gtcccgaaga gagcgagtgg gtggacggtg ggtgccgttt
    23941 tcctgaagat agggagtcct caatttgacc cctgaatcaa accggatgag aaagtaggta
    24001 aaatccagcc tgaagcccat tccggtactg aggtacaatt gtgataaaaa ttttgaactg
    24061 agttcgccac ccggcagaag aggatctttt tttaatatcc agacattgcc cccgtcaaac
    24121 aggatcgcac ctttaagaat ggagaaaatg tcaaagcggt actccgatcc gaattccagt
    24181 ttgaaattgc cggtctggta aaaactgaaa cggttattgc tggcctgttc attttttaca
    24241 atgccgggac cgatttcgcg gattgcccac ccccggatgc tgttgggccc gccgacatag
    24301 aactgcctga caaaaggaat ggcattgtcc tccctgccaa aaggaagggc aaggccggtg
    24361 gcaagccgca ggcctaggtc attcgtccgg gacagcctcc aggtatatct cgcatccagt
    24421 tccgtgcgca caaaccggga gatctttggt aataattgtt ttttggaaaa caggttgacc
    24481 gcgccggctt ccaggcctga ggtttccagg ctgatgagga tccgggaggc gttgttattg
    24541 gagtactggt tgttgatatc ggcggttatt tccttgatga agattccggt caggagccgg
    24601 tcctcaaaac ttttacggag gaattgaggg gccaggctgt cgaacccggg ggagaggtca
    24661 taattgagat aatcaaaacc gatgggattg aagcggtaaa agatgcggtt gtctttggag
    24721 aattcatacc caaaactggc tttggtttcc cgctggacgg attgttcgtt ccggccgctg
    24781 tagaaattgt aaaacagatc gtagatatta ccaatggata ttctggtcct ggtggttgtt
    24841 ttgatgtttt cgtaaaaacg gtcggacatc agcccgatct ggttggcaaa ccgtacaaac
    24901 ccaatttccc aaaaacgggg cacggtcagg ctggcctggg tgttgaggcc ccagggcacc
    24961 aggatttttt tagaaatggc ctgcagaccg atattgggga tgatggagac ggtaaactgt
    25021 tccgctccct taaacagatt gcggtgccgg tattgcaggg ccacccggaa atcaaacaat
    25081 ttgttgcccg ccgcctgatc gcggttggtt acgtaactgg attcaaggtc gacctggaaa
    25141 aactgttttt ttaccggcgg caggtgaagg ctgatatcca gcacatggtc gccggcccgt
    25201 tccgggagga tgctgatatt ccggtatata cccaggtttt gaagggtgtt gcgggtttgt
    25261 tgcaaggcgc tgagattgta gagctcatcc ccacggaatt tgaggtattt ctccagaata
    25321 tttggcctga gcgccatggc ctggtcgctt tcgtaatact ggatgaggtt gatcgtcgta
    25381 tccgtccggt atgccggatt ggacagccgg tcaaaggaaa acaccttgat ctttccgatc
    25441 cggaaaggtt caaatccctc ctgcgtctcc gcactgagga tggtgcattt caggtcaatt
    25501 gattgattgg tggagtcgga aaaaaaataa tcgaaattcg cctttcccaa ctggaaataa
    25561 ccggcatcct gaagcgtatg gatgatcctg ccctggtcca ggtagagcaa ttgttcgtcc
    25621 aggggaagac cgggctgcat gcggagcagg ggcttcaggg agtcgagcag accctggata
    25681 tgccgattag tgcaggtgta ggccatggat ttgactttaa gcgccctgcc cggggaaacc
    25741 gtgtaggtga cgcttgcttt tttgtttcgt tgggctacct catagagtac cgatccctgg
    25801 taatacccgg ccgcgttgag gtgtttgatc aggtcggact ggttttgaac gatctgggtc
    25861 gtgtcgagcc attgcggcct ttcggagagt ttattgataa acaaacggtc ccaccaggtg
    25921 gtatccccgg gatcgctgtg tttgaaataa aaataaaggt aacttttacc aaggccatat
    25981 tccgggctgg agatgacata tttgatccgg ttgcggtcgg cttttttccg ggtgagttcg
    26041 gaatcctcca gttttaccct gtttttggtc aggagtactt tcccttccgg gataaatttg
    26101 ttgatggcgc aggaggactg gatgaataca tacagaatca gtaaaaaaaa acaccgattt
    26161 tgccccgcga tacggataac ttttattttt gatttaaatg ctttcagaaa accatgcaaa
    26221 aataatacgt tccctttccc ataaaaagta tcgatacaag tataatcttt tcctggcaga
    26281 aggcgcgaaa acagtgtttg atattctgcg atccgggttc aaagaggttc agcaaatttt
    26341 tatatcccgg gcgcccgacg acccgaattc caccttgttg atgcctttca aagaaaaggt
    26401 gattgaggtg acggaccggg agatgagccg gatttccaac ctggataccc atccggagat
    26461 cctgatgacc gggagcatcc ctcctcccat ggtcccggaa gggtcctatt ttgaaaccgg
    26521 aatccacctt taccttgacc aggttcagga tccgggcaac ctgggcacca ttctgcgtac
    26581 cgctgaatgg tttgcggtcc ggagcattgg attcagtgaa ggctgtgccg atatcgtcca
    26641 tccgaaggtg atccaggcat ccatgggcag tttttgcagg gtgaaatact ggtgcggcgc
    26701 catatccgcc ttatccctgg acgatcatta tccggtgatc ggagcggccc tggaaggaga
    26761 atcattgtat cagaatttct tgccggattc gggtcttttg gtcatcggga gcgaaggcca
    26821 gggcatttcc aaacaggtaa gaacccggtt gacctgtact gtccggatcc cggcttatca
    26881 ggcgctccct gaatccctca atgccgcgat ggccaccgga atcatattgg cggaatggca
    26941 aaggcagttg cataccagaa atgacaaagg gcaacccatt gcctgaattg ccctttgccg
    27001 ggagtagggt gaatgttttc tttaaagcag tttttttact gcgggctcaa gggcggggcc
    27061 gcgcaggttg acgcctgcga tccggccttc ccgatccagc aaataagccc tggggatcgc
    27121 atcgactccg tacaatcttc cggcagcgct gttccagaac tgaagatcgc tcacatggtt
    27181 cggccaactg agtttgtcag cagcaatggc ttgtttccag ttttccatgc ctcccggccg
    27241 gtcgagagag acgctgtaaa ccgtaaagcc ctggtctttg tatttgttgt aaacgtttac
    27301 cacgttggga ttttcccggc ggcagggtcc gcaccaactg gcccagaaat cgagcaatac
    27361 cactttcccg cgaagggaat ttaattcgat cacttttcct tccgggttgg gcagggcaat
    27421 gttgggggct tccgctcctt ccttgatcag cattgcggat tctttggcca tcaggttttt
    27481 ttccagggtg gtggcgatgt tgtccaggtc ggtagtatag gcgcttttcg ggtgtttttc
    27541 cctcaactta gcgacaatgt ttttgagcaa atcgatgtct tccctggtct cattgatggt
    27601 acccaggacg aagaacaggg aagtcagcgg gttatcggtg gcagccaccg cctttttaag
    27661 gtcattcata ccgatttctc tccggaataa tttgccggcc agttcattgg ctttgatgga
    27721 ttcgtcagag cctttgatag aacaactccc gttgtccata tcgttcaggg ttccgttgat
    27781 ttcaatcact ctttcactgc cgtccaggat aagggtcagg ttacggcttc ccacccggat
    27841 ccggtatatg ccggcttcca tcgggacatc cgtgctcaag gaaaatttac cgtttgatcc
    27901 aatatccgac tgggcgacca cttccacctt ctgggtaacg atcggaagtt ggtcaaaaaa
    27961 gatccgcaga tttgaggcgt ccgaaaatct tcctttaatg gtcaggcctt tggaaccggt
    28021 ccctgcgcaa ttcaggccag agatcagcaa tccggcaccc aataaaatat agataaatcg
    28081 tttgatcata ttattaaaaa atttaatgca aaattaaaca ttttcccact cagtttcaag
    28141 acggttgctc ctggctttcc atcaggtgtt ccaggctgga tgcatactgc ttattccagt
    28201 ccgtcaccga tccgaagggt tcattgtcga gggtaaaaat accgtcctgc cgggtccttc
    28261 ccaatttgat ataaggaata aggtcaccag cgagggcttg tactacatca tcctcgcgga
    28321 tagggtccac gccgataacg atcctggact ggccttcccc gaaaagaaaa ctgtctttgc
    28381 ggatgccggg catgctgacc aggtcaaaac ccaggttgcc ccccagacaa gcttcaaaca
    28441 aacaaaccag aagaccgcct tcactcacat catgggcaga gaggatgaat ttatttttga
    28501 tcagcttgct gaccgccttt tgcacctgga gctccatgga aaggtcaaac cagggagccg
    28561 gagaatactg aataccgtga atttgcctga ggtattcact ggaaccgagg tcatcggtta
    28621 ctgggcctac caggtaaacc tccaggcccg cataagggaa agacaggttc gttttttgat
    28681 ctgcctgcag cagtccaagc attccgatgg tcggagtagg ataaaccgga atcgtttggt
    28741 cacccacctg tgattgattg taaaaactga cattccctcc ggtgaccggt gtctcaaaag
    28801 cccggcaggc aatacccatc ccttttatgg cttcaacaaa ttgccagtag acttccaggt
    28861 catagggatt tccgaaattg agacaattgg tgatggcaag gggagtgccg ccggaacata
    28921 caatattccg ggccgcctca gccacggcga tctgggtgcc tgtatttgga ttggcaaaaa
    28981 cgtaggcgct gttgcagtcc acggtgaggg ccagggcctt gtccgtacct ctgatccaaa
    29041 cgagggctgc atccgaaggt tccaccgtat tcattgagcc agtccttacc gtggaatcat
    29101 attgacggta aatccatttt ttggacgcga tattcagtga gcccaggagg atgctggctg
    29161 cttctcccag gtggtcgggc tggggcacct ggcgggggtc gaagcctgca attttttcga
    29221 gataaccggg tgccttgaaa gatcggtcat aaacaggggc ccccccgccc aggaccaggc
    29281 tgtgggcggg tacccgggcg acctcttccc cgtgaaaata atactccagg taagggctat
    29341 cggtcaccct gccaatctct gcacattcga ggtcccattt ttcgaatatt tccatcagtc
    29401 ttttttcctc ccctttccgg caaaccacca gcatcctttc ctggctttcg gaaagcagga
    29461 tctcataggg ttgcatatcc gtttgccgta agggcacttt atccaggtgg atgaccatgc
    29521 cggaattgcc tttggccgac atttcggatg tcgaacaagt gatgccggcg gcgcccatgt
    29581 cctgcatacc aatgatgcaa cccgaccgga tgacctcgag ggaggcctcc agcaggagtt
    29641 tttcctggaa agggtcgcct acctgcaccg aaggaaggtc ttccgcgctg tcctcggaca
    29701 ggtccgcgga ggcaaaagtg gccccatgaa tcccatcctt gccggtcgcc gaacccacaa
    29761 tgaacacggg attgcccgcc ccatgggccg tggccgatat ggtttcgccc acttttacga
    29821 ttccggcaga catcgcgttc accaaaatgt tttgctggta acaatccata aaataggttt
    29881 cgcctcctac ggtgggaacg ccgaaacaat tgccgtaatt accaatgcct tttaccacgc
    29941 cgcggaccag gtttttcata tgggacgtgt ccggcaggcc gaacctcaat gaattcaggg
    30001 cggcaatcgg ccgggccccc atggtaaaaa tatcccggtg aatgccccca accccggtcg
    30061 cagcaccctg gtagggttca atcgctgacg gatgattgtg cgattcgatc ttaaaagcgc
    30121 aggccaggcc atcgccgatg tccaccaggc ccgcgttttc ttctccggca ctgaccagca
    30181 gggatttgcc ttcgcggggc aaggtcttta ggtatttgat cgaattttta taactgcaat
    30241 gttcggacca catcaccgaa taaatactga gttcggtata gtttggcagc ctgccgagga
    30301 tttgttggat cttttcaaat tcttcagggg tgaggcccag ggcggtggcc attgtcaggt
    30361 tcgtttcagc ggttgctaac atggggcaaa gatagagaga aaccattgca acccggaatg
    30421 tggctgccta ataccagttc tgaatcttca gagcggttgt gccggcgggt aaagtaagga
    30481 tagcgtcgtg atcccgcatc gccggatacc cactgacgac ggcctgcatc cggcatatcc
    30541 tgaattttaa ccagcaatcc ttgccataat gtttcaaagc cttataactt tggctttcaa
    30601 taccatgaaa ccatgaaaaa tattatattc ctccttttga taattacccc tttaagcctt
    30661 cttcaggcgc agatccagaa agaccacatg ctcagcgtga cgatagatgg taaagaattc
    30721 accacacagc ctcagcatat caaagtgggc gcctaccgtt acattaccgg caacgccgtc
    30781 aaaccggatc agtccctgcg tatctggatc gggagctggg acgggggatt tgtcagagaa
    30841 tccggcagtt atcttatcgt caacggcgat aaaccggata ccaaggaaaa tttcaaaacc
    30901 gcccaggacc tcggcaaata caaagggatc gcggccatca aatatgtcct ggaaaccaaa
    30961 tcgcccagga tggaatacca tgtagggtac agccagaaca acgatgaatc cctggacgtc
    31021 aaaatcggag aggacggcta tatggaattc accttccatt gcaccctggc cggtacctgg
    31081 tggaaggaaa aaacctcagc cacggtcttc ggaggactgg gcaggatcgt ggataaaatg
    31141 gaggacaaag cggtcaccag cgctaccggc ttcgaacagg atatcgatcc cgaaggcaac
    31201 ggatataaaa aacaaaaaac cacggacaag atcgaattga aaaacggggt ggtcaaactc
    31261 aaaatgaagg cggaataatc cttcaccgga tcacaaacca ggtggaggaa gctgccggta
    31321 ttcttactct gacttttatg gaatagtccc ggtcaatccg gtatttgacg ggacgtccgt
    31381 ccttttccgc cagaattgct tttgtggtca gaccggtata atacaatgga atccgtatct
    31441 cccgttccag ttcatggttc aacggattaa agacttgtaa aaatcctttg tccttaaggc
    31501 cggggttgac gtgcaaccag ccatcccagt ccctgccgtc tgcacggcga aggtggatca
    31561 ggtccgagtt caggatttcc ctgtatattt tgtaccagcc aatgatccgg atgaccatgg
    31621 attttgtttc ttccgtgtca aagagccttg gtccgcggta gcaggcctgg accccggcgc
    31681 cgtaatattg ggtcatcagt ttctcatagt cctccaggtg tgcatgcagc ggctcgagta
    31741 cggcttccgg cccgcctccg tgatactttg tgagtggaac aaaaccccag gccatggaac
    31801 tgtttttttc ccaggtgccg tcatagatgt tttgccggtt gaggaggact tgctgttccc
    31861 ggggcagtga aaaattgact tcccggtatc ccagggcgat tttatgggtt ccatcgagga
    31921 aataccagtc cggagcattg atatagatgc ccctttccgt acaccagcga tagaggtcct
    31981 tttgcatttc catctgtctc cactggctgt cttccaggcc ctggtgtccg ggatgatttt
    32041 ttgaagcgca caggtccccc ggatacggcc cgtcattttc aaaaaggtca aaaccggtgg
    32101 cctggaaaaa cgttttaagg cgtgcagaat aagcctgacc ccaggcgctt ccgaagcagg
    32161 gggcattgcc gaaaaaagca cccccgggtt tacccgtagc ggggttgatc acgtcttcct
    32221 catcgctgat cctcctagag ctgaaaaggg aatagctgcc gataaatata ttcttggaat
    32281 gggcatagtc tgccagggtt ttccatctta tgagattggc agcggaagta tcctcgatgt
    32341 tgcagtgact gccaaagctc aggatcagcg cttcataacc cgttgcggcg cattggtcaa
    32401 tggcctggcg cacttcttca tccgaccggc tcaccaggtg cataaaaatg ggattggtat
    32461 tggtccaggg agcgatgacc cggtacattt ttctgactgc caggcccctg cgctcacggt
    32521 catagctgtc caggaggagt tcataggtcc tgatggattc aaaataagct ccggcaggca
    32581 gttcgatccc gggtccatga gtggggtata cttcaagcag gcaaggggtt tcataattgt
    32641 aattcacctg ggaagtgtaa ctgctgtcgg ttttccagtg ggtcgtctgg tcactgaggg
    32701 catacctcat ggcattgttg aaagcgtaat tggattcgat atacaggccc tggggtgttt
    32761 tcatctgatc cggcttgcca atcaccgcgc tttcttcttc taccattgcc aggatttcat
    32821 tgatcacccg gtcaatccgg acggtttgcc ggccgtcgtt acggatgctc acccatttgc
    32881 agatcagcgg gaggccgtca tacaaatcat agtggacgga aaccttcagg ccttgaaacc
    32941 ggggatgccg gtattcaaaa ctcacggttt tgcccgaagc tttttccctc tccatatgcc
    33001 acatggacgt cttccagttg atccttggac ttatccctgc aatggaataa ttggtaaact
    33061 ggaagtcatt ttcatggccc gacagggaat ccatccattc cgccctgagg tatgcctttt
    33121 ctttttgtcc atacaagcct ccgatgttaa aggtttcccc gtccaggacc aggcgggctt
    33181 cagggcttgt tgctctcagc agttgttgct gattggtcgt attggtgaaa tctatgcaag
    33241 ccagatttgg ttgcagccgg aatacccttc gcaataagcc gttgtaaagg accagttcct
    33301 tcccctggtt ttgtgtaaac actcccgcgg gttccgttat gggggtgacc aaccaatccc
    33361 gcccggatga gctgtggctt tcagggttcc aggcagggac ttggtcctgg gcattcagaa
    33421 tgcaggaaca ggaaaaccat gaaaaaaata caaggaatga atttttcatc ttgacaggtc
    33481 cgtattccgt atgcagggat cccggtatgc gggttttcct gcgcacaaca tgtttccaga
    33541 gtttaagatg ccgtattccc ggtcaatcaa attttctacc gctgcggtcc gtaaaattgc
    33601 tgcccgcttg ctgggtgccc atgaccaata catcctggat attgatatgg ggaggacggc
    33661 ttgcgacaaa atagatgatt tccgccacat cggaagattt caacggatta aaatcttcat
    33721 agatgctggc ttttttctca tccccatgaa accggacccg ggcgaattct gtttcttcga
    33781 ccatgccagg agcaacctgg ctcacccgga ctccgtgagt ataaaggtcc aaccgcatgc
    33841 ctttggtaag catgtccacg gcggctttgg tggcactgta cacattgccc ttcgggtaaa
    33901 tatcctttcc cgcagtagaa cagatattaa tgatatgtcc cgttttgcgg gctaccattt
    33961 ccgggctgat caggcgggtg atgtagagca aacctttgac attggtatcg atcataaggt
    34021 cccagtcctc cagacttcct tcatggatat aatcaagtcc gctggccagg ccggcattgt
    34081 tcaacaggac atcaatgggt ttgaattcat ccgggatctg gcctattgcc gattctgttt
    34141 cggccaggtt cctgatgtca aaagacaggt tatggacctt cacgccgaag gatttccgga
    34201 ggtccatggc caaattgtcc aaacggtcct gtcttctggc gcacaaaatc aaagagtgcc
    34261 cgtgacgcgc aaattccttt gcagcggctt tcccgatccc cgaggaggcc cctgtgatga
    34321 ttacattcat tttatttttt ctttaagggt ttgaatggtt tctccgaatt ccctgacaaa
    34381 ttccgggtat ttgctggtcg ccggaccgtc aaaacccgta tgaatcgaat ggatcttatt
    34441 gtttttatcc aggatgacca gggtggggta ggcgaggatg gaatccagga agggcagctg
    34501 tttactgacc accgctttgc tggttgtggt ggcagccagc aaaatctcat agggcacttc
    34561 cagttttttc cggtaattgc ggatggcatt caaggctttg gtggtatccc ggtgcctttc
    34621 acatgcgagg ccgatcacgg cgatatcggg ctccaggttt ttttgcaaat attctttgag
    34681 aaaacgggtt tcatcataac aattcggaca ccaggttccc atgatctgga ggattttgat
    34741 gtcctggtca aagtccgcca gggaccgggt ttcgccttcc gggttcagga agttgaaatg
    34801 aaccggcagc cccgtttcca tatttctccg ggttgcggag gatagggtgg cgttcgggtt
    34861 ccttaccgct gtccaaacgg acagaaaatg cttgcccgat ttaaaggtgc ccaagaggct
    34921 gtccccggca atcttgccaa agaacaggta ggcatgtgag ccgtcaaagg cggagagaaa
    34981 aaatttattt ccggtcaccg ttccttccag ataccggtga tctcccgttt cggtcctgaa
    35041 ggtgccggtg agggaggcgc cattttgttt aaattcccca atggccttct ccgttttttc
    35101 caggttaagg tcaaaaagac attcccaggt accggtcagg tccgaaaccg gatcggaggg
    35161 caccacctgg aaacggtctt ttttaccgaa atgcgcattg aaaggaatgg aataatttac
    35221 gcggttccgg gccacccatt ctccctggat gatcccttct ttcagaatgc cgcggatatg
    35281 ggaatcaaaa gcgacaaatt cgatccacac ggtatcttcc gcggtatttc tgttcctgcc
    35341 ggtgtaaact tcgtcgatcc ggatccgttc cgaaccattg atgatttcaa tatagggttt
    35401 ctggtcttca ggcctgtaaa agacttcaaa aaggaagggc agttcatctt ccgggtcgta
    35461 taccggttgg gtgccttcgt tttcataacg ttcgatctcc tccttgagag aaagggtggg
    35521 gatggtgctg agcttcaatg cgcccctcca gattccgggt gccagggacg tgtaggattc
    35581 cttcggccgg atacagccgc cgatcagggt gtacacgagg agggaaaaca agcatagccg
    35641 gttcacgatt cggatttttc gccgacaaag gtatcctctt tgtacttaaa aagaatgttg
    35701 aagctggtca ggctgccgta gatcctggta atatattgtt gcatttccac cttttccgca
    35761 tcggtcattt tgggatgggc attgatcttt tgttccagga ccctcaaccg gtccctgacc
    35821 atcacgattt tatggaaaaa agcctcaatc ggcatttctt tgggtttcag tcctggttcc
    35881 gatggatgca gcaccaggag gcctttgtcc cacttgtccc caatcggggt gatcgtggtg
    35941 atgtcggacc actgacgcag gatgcgggcc agtgattttt ccgcttcggt ataggtgacc
    36001 gcttcatcag cgggtaattt ttcaatgatc tcccaggccg tatactcctt tccaacccct
    36061 ttgatcccat aatggaggaa acatacatcg taagcgacag ggtgcaaccg gacgatcact
    36121 ccttccccat aagcgggatg tttaactctt gaaccaatac ccaaaaggtt ttccaatacc
    36181 atgctattcg attatttttg taaaattctg aaaatcgcat gacttattat tattttaagc
    36241 aaaggatcct tcttgcctgt gtggcgggtt tcctgggtgt cacgacctta tttgcccaga
    36301 aaacggtgaa gaaaatcaat aaaaaagtag atcaagccac catccaaaag gatatgatgt
    36361 acctggcctc cgatcaactt cagggaagga agacgggcga agcaggcaac cacctcgctg
    36421 ccgcctatat tgccggagag ttccggaagg ccggggtgag gatgatcccc ggcatgaagg
    36481 actatttcca atccattcct ttccagaaat tcatcccgcc caaacagggg gaggtgatgg
    36541 tgttgggttc cgactttacc ctgggttcca acctggtgat cctgtacgga gggcccgcga
    36601 ataccaagac ggaagcggta tatgcagatt acggctggat tgatgaaagt gctggacggg
    36661 atgattataa aggcctggat gtaaaaggaa ggatcgtgct ggtccagggc ggattgccgc
    36721 aagggggatc gccgaccgaa atattcgcgt ccatggccaa aaagagggaa atggcccggt
    36781 ccaggggcgc ggttggattg cttgaaatct ataacctgag ttttccctgg aattttttcc
    36841 gttcctattt cagcagagag cggcttgagg tagttccaag tccgtccacg gaaaacgatt
    36901 cttttttcca tgcctggctt cagttgccgt caggagccaa accctcgttg gaaatcggta
    36961 aatccttccc gatgcaaatc aatacctccg gggccctatc cagccccgtg caggcagata
    37021 acgtgctggg ctacatcccg ggtaaaaaac ggaaggatga atatgtcatg ttgagcgccc
    37081 attttgatca tgtcggcacg agagccggcc aggtgataga caacgatacc atttggaacg
    37141 gtgccaggga caatgcctgg ggcactgtag gcttgatcgc ggccgcgcgt tacttcgccc
    37201 aaaaccctcc tgaccgctcc atcctccttg cagccgtgaa tggggaagaa atgggcctgt
    37261 tgggttccaa atacctggcc gaccatcccg ttttgccctg gaagcaggtg atttttaacc
    37321 tgaattcgga cggggcgggc tattcagaca ctactattgt atccgttatt ggtttaaacc
    37381 gggttggcgc cgccgctgaa ataaacgccg gctgtgaagc ttttggtttg gccaccttcg
    37441 cagatcctgc accggagcaa ggcttgtttg accgttctga caatgcttct tttgccgccc
    37501 tgggcattcc ggcacctacg atagcggccg gactgaggtc ttttgatgag accgtccgga
    37561 aatattacca tcaatccatt gacaacccgg acaatatcag ttattcctac ctgacccgat
    37621 ttaccaaagc ttttgtaaat tcagcaaaca atattgccaa tatgaaagaa aaaccaaaat
    37681 ggcagagcgg ggataaatac gaggcggcgg ctaaaaaatt gtacggttat tgaataccgg
    37741 ataaatttta actatccttt agactaaaat tgttaggata tcgttaagga ttgcatgggc
    37801 ttggccatgc gcgcctgtga aagaagacct tgatttctaa ttttttagca tggtttttgc
    37861 catatgaggg atactgtatt tccttaacgg ggtgtcagaa tatattattt tgcagtacaa
    37921 aactatacac attgaatttt atagccatat aaaataatat taatgaaacg aattctttta
    37981 ctgtctttat tttttgcggg tgtaattatt ttaacaccaa aaaatatcaa ggcccaggac
    38041 ctgcattatt cccagtttta taatgcgcat gtcaatatca atccgggcct gaccggcatt
    38101 tttaacgggg atgtccggat tgccgccaat taccggaatc aatggaaggt gctccccatt
    38161 ccttatatga cggtcaccgg ggcttatgat atgaaaatat acggtaaacc ggaccgcaaa
    38221 gacttttttg gagcagggct ggtgtttaat tatgaccagg ccggggattc caaacttaat
    38281 ttggcgaccc tgggattgaa tggttcctat tccctgccgg caggcaataa ctcttttttc
    38341 actatagggg cccagctcgc tttctcgaga agggcttttt ccctggatga cctgaccttt
    38401 gatgaacagt ttgacgggct caaattcgat cctaaccgga cgatcacgga ggcttttgac
    38461 cgcaggtccg tctttgtgcc ggatttttcc gcagggctca attaccgctt tcaaaaatct
    38521 tcgcggacaa aggctgatat cggggtcgca gcctttcacc tcaataatcc gcggaatccg
    38581 ttttataccg atgataaatc ggtcaaactc cctccgcgct ttgccttcaa tgccctggtc
    38641 caggccaagg tggccaatcc ggtggatata ttggtgcatg gcctggttca gtttcaaaaa
    38701 ccacatcgcg aatacgtcat tggggccggc cccaagtttt acctcaacca gaagagggga
    38761 aaaaattttg cggtcggttt gtttggcatg taccgcttca aggatgccct ggcccctgcc
    38821 tttcacctct atcttaatga attgcaggtt ggcttcagtt atgacgtaaa cacttcagcg
    38881 ttcaagacgg tcaccaacaa gaggggagga ccggagcttt cgctgatcta tacgattacc
    38941 aaggtcaaac ccctgagtaa atttaaagcc tgtccgattt tttaaaaatg aagattatga
    39001 actggaagaa actatatatc ccgctgttgt tggccctctc ggtttatttc ccgggccaaa
    39061 gtcagaccta taaggctttt gtaaaagcag ccgatgaagc cgccgacaag aaagaccatt
    39121 attcagccct tgtatacaat ctgaacgccc tggaatttga cagcacccgg atcgaccgcc
    39181 tgcacgccgc agcggaagcg gcccgaaaca tcaattccta taaagtggcc gagaagtatt
    39241 atcagaaagt cgtggagctg gataaaaagg agacttatcc cctggacaaa ttttacctcg
    39301 ccgaaatgaa acaaaggcag gggaaatatc cggaggccaa agaagtctac cagatgtacc
    39361 tggccgaaaa ccggggagat gacgcttatt tcacccagaa gtcggaaaag gaagtcaagg
    39421 cttgcgactg ggcagcggac caggtcgtcc agacccagga aaatgtcagc atcacccgta
    39481 tgggagacga tgtcaacaca ggttattctg aattcggcgc gctgagaaaa ggcgacagcc
    39541 tgttttattc ttccttaaga tttgaaatgc ctggagatcc ttcttacccg agaaaaacct
    39601 attccaacct tctgcttact gtaaaagggg ttgccagcga agccctggct cccatcaaca
    39661 gcccgctgac ccatactgcc cacacggctt tcagtgaaaa aacgggcagg ctctatttta
    39721 cgctctgcga atacctcaat tccagcgata tccgctgtga cctgttttac cgggaaaacg
    39781 tcaacggtca gtttggggag gcggtaaaac tgccggaacc catcaacctt gccggattca
    39841 ccaataccca gcccaatatc ggactggacc cccaaaacct tcaggaaacc ctgtattggg
    39901 tatcggaccg taccgggggc aaggggaaac tggatatctg gtatgcccag gtcaatccgg
    39961 acggtagttt ttctgctccg gccaacctgg cttcgatcaa taccgccgaa aacgacatca
    40021 ctcccttctt tcacaatggg tcgcaggaac tttatttcag ctctgaaggc tatatcggtt
    40081 tgggggggta tgatgtttac aaatccattt taaggaataa aaattggtcg gaacctgaac
    40141 accagggagt gcccatcaac agcagtttca atgatgttta tttttccttg gcccccgatt
    40201 ccagaagagg actgatgtct tccaaccggc tcggttcagc ctatctggat gaactgcaag
    40261 aggcctgctg ttatgatata tacgatgtgg accttgagcc tacccgcctg gacctggagg
    40321 tcaccacttt tgaccggcat accctggagg ccctggcggg ggcgacggtg aggttaaaaa
    40381 accttaccaa tcccggagtc ccggatgtgg tgttgaccaa tcctacggca aacgatttca
    40441 aatttgcatt ggagcgcaat aaagattacc tcattacggc taccagggaa aaatacaggc
    40501 cggataccgt gagtgtttcg actttcggga tcagccgttc gcagacttta tccaaaaagg
    40561 tatatctcga gaccgatatc atagacctga acgtgctcac ttttgacaag aagacccttg
    40621 aagaattgca gggcgcagag gtgaccctga tcgacctgac agacagttcc gcagtgacca
    40681 agatcaaccc cagcggaaat gacttccatt tccccttgat acgcgggaga cagtaccggg
    40741 tcattgccaa taagaaaggc tataacccgg cgacactctt gctggatacc cgggccatcc
    40801 aggcgaatac cattaccgag aaaatgtacc tggatatcgg agatatctat tccttcctgc
    40861 ccctgatcct gttttttgac aacgatcagc cggatctccg ctcctaccag ccccgcacgg
    40921 aaaagttgta cagcgaaacc tatcctcctt attatgcacg caaagaagaa tacaaggacc
    40981 agtgggcaag caaattaaag caggaggaga tccctgcaag cgaccgggat tatgaaaatt
    41041 tcttcaacga caggctgcgc aagggcaaca acgatttgtt ccggttcctg gacatcctga
    41101 tcgggatgtt ggagcgaggc gacaaatttg agatcttcct gaaaggatac gccagcccga
    41161 gggcttctga acgatacaac ctgatcctgg gtcagcggag gatattcagc gtgaaaaacg
    41221 aattcctgaa gtaccgggaa ggggtcttaa accaatacct gaagaacggg cagctcaaga
    41281 tcgcggaaaa atcatttgga gaaacgaccg caccccgcag agtgcctgac agcatcacgg
    41341 atcaaagact ttcgatcttc agcctggatg cctcccagga aagaagggtg gaaatcgtcg
    41401 aagtcaaacc aagtaaatag aaactaccat gaagaaaata tatatgccta tcatcatgaa
    41461 gacctacagg agcctgctct ggagtctggg cactttggtc ctgatcctgt ctgcccagcg
    41521 tgcacaggcc acccatcttg taggagggaa tatctcttat cgttgtataa gcaaggatat
    41581 ctatgaagtc acggtcattg tgaggcggga ttgcctgctt ggggatcccg aagcctattt
    41641 tgacaacccg gcttccattg gtattttcga taaaaacggg gaactgcttc agttcctggg
    41701 cgtgcggggc caacttttat tgaaacttca ggtcgacgac acccttacac cggccatcac
    41761 ggaaaaaatc tgtaccatca atggttcgca aagcgtctgc gtgcacgaag ccacttacaa
    41821 aggaagcata cacctgcctt accgggcagg gggctattac ctgatgtacc agcgatgctg
    41881 ccggaatatg cccattcaga acattacgga gcccctggaa acgggaggtt cctgggtagc
    41941 ctatatttcc gaggaagcca tgaccgcctg taataccaat ccggtgttta aaaactggtc
    42001 acccatcttc ctctgcgccg gatctacttt caacttcgac catggagcca cggacaagga
    42061 cggcgattcc ctggtatata aattatggac tccctatacg ggcgcaaaca ggactttccc
    42121 cttgcctcag ccgccaaaca aaccggactt caacccggtg ccctggcaag ccccatacag
    42181 cgaagcggac atgatgggcg gcgacccatt ccggattgat tcacggaccg gtatgctcca
    42241 tgcggttccc aataccatcg gcgtattcct gcttggcgtc caggtcgaag aataccggaa
    42301 tggtaaactg atcgggaccg tctaccgtga ttttgaagtg caggtcatag attgcagtaa
    42361 tctcattgat gccaaaatct ctgccccggc cctccagtgc gatgaattgg ctgtacagtt
    42421 caaaagtgaa agcatcaatg ccacccatgt tcgctggtat tttgactttg accgtaacct
    42481 caacgccacc agtacggaaa taaatccaac ctatcaatat acggacaccg gtacataccg
    42541 ggtagccctg gtgatcacca aggacagcgc ctgtttcgat accgcttatc atgtggtgca
    42601 cgtcaaatca tcggcaagca tcaaggcggc ctttggggta aaaaccggac cctgtaagga
    42661 tggtttcatc ccagtgagcc tcacggacca gagtacgggg gtagatccga cgggcgtata
    42721 tacctggacc gtaacctatg gcagcaccac cctcacttcc tctgaaaaaa atccggtctt
    42781 ttcggtcccc aacggagtgc tggccgtgat caggctggac attgtggagg aaggctccgg
    42841 ctgcttggct tcatccaccc aaaccttgct ggccaccttc atacaacctg gcgatcacct
    42901 ggattcattg agcgtttgta caggcgatac ggtcattgtg aactttattt tatccgattc
    42961 gatccggggc aagtatgatt acacctggga ccccagcccg gtcatcatcg ggggagccaa
    43021 tacacccgaa ccggtcatcc tttcacccgt agaccaggag gtttacatct atgccaccgt
    43081 ggacaataag gacggttgca ccggcaggga ttccatcctg atccggtcgt tacataaacc
    43141 aaaactggca ttcaccttca gtaatgtttg cggttccctg ctggtgaaag tgatcaacca
    43201 gggagatacg ctggaccact accattggga ttttggagat ggtaccactt attcggtgcg
    43261 ttcgcctgaa cacacctata ccatcgcagg caggtatacc gtgaccctat ccaccgccga
    43321 tgattgcggg accagcctga gtaagacttt tgatatcggc aatttcccct gtcttgaact
    43381 caaggatacc attgccggct gtgaaggcag aacgctgcag atcaatcctt tcgcgaatcc
    43441 actccatgga tacctctgga ggccgacgga taaactcagt gatctgaata ttcccaatcc
    43501 atcctttgtg atggattcct cgcgcatttt catcgcggat atttatgatc tcaataccgg
    43561 tattggactg ggtacccata ccatcttcgt catcaacctt ttgtccgatg agatcgacaa
    43621 aatcagggat accgtgctgg cttgcctcgg acaaggggtg cccctcaacc cggagggcaa
    43681 tccagccctg aactacaact ggtcgcccgc ccagtttctt gacaatccca cttcggccaa
    43741 tccgttggct acggtttcca gccagacggt atttaccgta atgatcacca atccggcaga
    43801 ctcctgtgtt gccagttccc aggtggtggt cgtcgtcccc attgccatcg ctgccgagat
    43861 cattccagac agcctggaag cctgcctcaa tattccggtg gcactgaatc ccaacgggat
    43921 ccaggacccc aatctggaat acctatggtc cccggccgac aaactggata atccggccag
    43981 tgtgaatccg aaggctacga ttgacagccc taccgtgttt acggtgacca taagcgacaa
    44041 acgttttgaa aactgtcaga tcaccaaatc cgtagtagtg gacattccgc tgatcgaaga
    44101 agtggataag ataaaggaca gccttaccgt ttgcgccggg gtatccacgc cattaaatcc
    44161 gggcggcgat ccccggtttg tgtacgaatg gagccctgcc gaaggactga gcgatccgaa
    44221 tgttcccaat cctaccgtga cccctgtcgc gaccacggta tatacagtaa aggtcaccga
    44281 tacccaaaac gggggatgtt tccttacgaa agaggtaaaa gtgattgtcc ctcctggttt
    44341 taccttggtt cccgcgttca gggacaccgt gagttgcaat ccgactggtt ttaccctgaa
    44401 ggtttcctcc gacaactcca aggtgacttt tgaatggttc aatccggcag gagcgtcgat
    44461 aggaaccggg gatactttga atgtaaaccc cacccaaggc actatctacc gtgtaaccgg
    44521 aaccgacgag tttggttgtc agaaaagcga tacggtaagg gtcattcctg ccccggttcg
    44581 catagccatt tcggttccag ggggagggct ggtatgccag ggtgattcga tccgactgac
    44641 cgtgaccaat cagaatcctg cccaggtgct gacctacagt tggacgccaa ccaccgatat
    44701 tctgagtggg gctaatacgg ccagtccgct gattcgtccg acggccagca ggaaatattt
    44761 tgtaacggcc accaatcaac aaggatgctc ggcagtcgat tcggcagaga tcaggttcag
    44821 cccggttcag gaagtgacgg tcactgccaa tccgaccacc atagtgatca gtcaggagtc
    44881 gcagatcacg gccacgaatg tgcccggcta tacctatcgg tggtcccctg ctgaaggttt
    44941 gagcaatcca aacataccca acccggtggc aaaacccacg caaacgacta catatactct
    45001 gacggtgacc aatccggacg gctgtgagca aatccgccag gtgaccatca ccgtccagac
    45061 cctgccctgt gcccggccaa acatattcct gcccaatgca ttcacgccaa acggagatgg
    45121 aaagaacgac gtgttatacc tgaggggagt gatcgtagag agcatgagcc tgatcattta
    45181 taaccggtgg ggagagaaag tatttgaatc gagaaatcaa agtgtaggct gggacggcac
    45241 cttcaggggt aagttgctgg acccggacgt ttatgcctat tatctcaccg tgaattgtat
    45301 cggaggggaa gtattcaccg aacaaggtaa cgtgaccatc ctccgttgac ggagtccatt
    45361 catcaataag gcctgtatgc cgcggaggct tacaggcctt attttcagga tcagccttcc
    45421 attttagcag gagtgccttt ccggtaggat tcggtcattt ccttggaaac cgcggaccga
    45481 atcacattga cgtaggattt cgtgtcgatc tggagggaaa cgatctcatt ttcaatttta
    45541 ttgacccggc cgatgatgcc actcatggtg accacctcat cccctttttt gatctcgttt
    45601 tggaaagcgg tttgggcttt ctgccttttt tgctgaggcc ggataaagaa aaaatacata
    45661 atcaccagca ggagggggaa catcagtaaa ttaaaaatcc cggatccgcc actggcctgt
    45721 agaataatga gttgagtcat gttgtacttg tttttagggc aaaaataatc atattgcacg
    45781 ctcaaatggc atcggtcatc tttttaaccg gttttatggg ttcgggcaag accgctgcgg
    45841 gccgcttgct tgcaagccgg ttagggtatg atttcctgga cctggatgac tggattgaaa
    45901 aaaaggaagg ccggaccatc agccagatct ttgcctcgga tggggagggc gttttcaggg
    45961 acctggagca aaaagcactc cacgaaacgg gcaaccggga acggatggtc atctcctgtg
    46021 gaggaggaac cccttgtcat cataacaata tggaatggat gaatcagcat gggttcacgg
    46081 tattcctcaa tccctcaagt caggtattgg ctcaacggtt gttatccgga aaggcccatc
    46141 gccccctgat ccgggacctt gaccgggaag gactggtggc ctttattgat gaccggctca
    46201 aagaaaggat gccttattat acccgggcca ggctgcaact ggtaggaatt cacgatcagg
    46261 aaacggtggt cagcgccatc ctggaagctt taaaaccgga gatcagtttt ccggagggta
    46321 aagcttgagc agtcggtcaa tagccctgcc ggcatggatg accggcccgg tagattcagg
    46381 gtctccgctg aggcttccgg tctccgccaa aatggaatag acctcttccg tggagagggc
    46441 cggctggatg ctcttcatca acccgacgag gccgctcaca cagggagccg ccatgctggt
    46501 cccgttgaag gaggcgtatt gattgttggg gatcgtgctg aaaatggaga cacccggggc
    46561 ggcaatttta tttttgatgc gggcgacgga gtttgaaaaa gaggctttat tggaggaggt
    46621 gtcgatggcg ctgacggtga tcaccccatc ggcatttgcg ggggcgaagc cccgggcgtc
    46681 ggaattgtcg ttgccggccg ctacaaccac gatgacgccg cgtgcattgg catagcggac
    46741 ggcttcagaa taggcttttt ggcggctgtc tatgcttctg ccccccaggg aaagattgat
    46801 cacatcggct ccctggtcgg cggctttcag gatcccgttg atgatatccc tttgggtacc
    46861 catgccgaaa ttgcccagga ctttaatgct tgaaaccgag acatactcgg ggcccgggtt
    46921 gagggatgaa atgccgacgg cgttgttggt taccgcggcg gcgattcccg cacaatgggt
    46981 cccatgtcct ttcggatcat cgtcgaacct ggactccacc gattcataat ttttattcag
    47041 gtcttcgtgt ttgccatcca ccccggtatc caggataaaa atgcgcgcct tctttttggc
    47101 aatgatccgg ccctcgcgaa attggctcat gacctggttc cagcccaaca attcaatcga
    47161 ccattgttta tcggtatgcg ggtcgttggt caggggcttg aaattggatt tataaggcgt
    47221 gatggggatc accggtattt ccgagaccac ttcattttcc tcgacccagg tcaccgattc
    47281 cagggcagcc agttcctccc ggatcgccgg gatcaggttt tcccggtctt caggcacttc
    47341 cagggcgact acattgtcca ggctggtgcc gacctcgtcc accattttaa acgccgtttc
    47401 tatgcccagg tggtattttt tgatgatcgg ggccaggatt ttacggtctt tgtccttcag
    47461 ttcaagcagg atctcccatt cattttccgt ataagaaagg ctggcctggt ggacggaggc
    47521 gcgctgccat tgaccgataa aaagcctgga taaggcgtat aataagatga ctgccagcca
    47581 atacaatcgc ctgaactcgc gtaaaaactg aaacatcagc gagatgaccc ccatgatcag
    47641 caagtccctg gacaggatga ccagtttttg ccctgcggac ttgggcagga aggaaaagga
    47701 aatggcatac accgatatcc cgatgacaaa aagtaactgg aaaagtttga taagaccttc
    47761 ccggtcccgg aaataatgcc agaggatcag cgcgaaactg gccagccaaa aaccatttga
    47821 ataccagtgc acgaggttat tcatacaacg aaataaccgg attccccggg atcaaggaaa
    47881 aaaattcggt caacatgtgc tttttaaaga cgaacagggc ctgaggccca tatcttcagg
    47941 tttcatcagg aattgcttat caaaaactgt acctttgcgg actcaatttt tatggagaaa
    48001 catcattgtg taatcattgg ttccggaccg gcgggctata ctgctgccat ttatgcgggc
    48061 agggcaggga tggatcccgt tttgtacacg ggtaccgaac cgggggggca gctcaccctg
    48121 acttcggagg ttgaaaattt tcccggatat ccccacggag tgctcgggcc tgagatgatg
    48181 gaggatttcc gaaaacaggc agagcgattc ggcaccatcc tccgctatga gcgaatagaa
    48241 aaggtggatt tcaccggccc aaagcacttg ttgacctcgg aagccgacct ggagatcgag
    48301 gcggacgccg tgatcattgc caccggggca agcgccaaat ggctgggcct gccgtctgag
    48361 aaaacttttt taaacaaggg agtcagtgcc tgcgcggttt gtgacggttt tttcttcagg
    48421 ggcaaggacg tcgcggtcgt cggcggggga gatacggcca ccgaagaagc cctgtatctt
    48481 tccaaattgt gcaagcaggt gcacctgatc gtccggaggc acgaactccg ggcttccaag
    48541 attatggaag aaagggtcat gaacgccgcc aacattcaca tctggtggga tacggagacc
    48601 caggaaataa ggggcgacca gtctgtgcgg tccattttat tgaaaaacaa ccagaccgga
    48661 caaaccaccg aactggaagt cagcggcttt tttgtagcca tcggccatca gccgaacact
    48721 tccattttca aaggttggat cgacatggat gaaagcggtt acctcctgac cttaccgggc
    48781 acttccagaa ccaaccggga aggggtcttc gcttcagggg atgcccagga taagatttac
    48841 cgccaggccg ttacggcagc cggctcgggt tgtatggccg ccctcgatgc cgaacgttat
    48901 ctgagcgcca ggggaattat ttgatttaaa ttattaattt tcgatatatc atgatcagca
    48961 caaagctaaa accgggggtg atctttggtc cccaactgaa ggaattatat gactatgcca
    49021 atgcggaagg cttcgccatc cccgcggtca atgtgattgg caccaatacc atcaacgcgg
    49081 tcctggaaac cgcccgggag gtgaacagcc cggtgattat ccagttttcg cacggagggg
    49141 cttcctttgt ggcaggcaag ggccttaaaa atgaattcca gcaggcctcc atcctgggag
    49201 ccgtttccgg ggccatgcat atccacattg tagccaagac ttatggggtg ccggtggtca
    49261 tccataccga tcattgtgcc aaaaaactgc tgccctggat cgacggcttg ctggatatcg
    49321 gcgaaaaata ttttaaaacc aggggtttcc ccctgttcag cagccatatg cttgacctta
    49381 gcgaagaacc catccatgaa aatatttcca tcagcaagaa atattttaag cggatggcgc
    49441 ggatcggcac gggtatagag attgagctcg gggtcaccgg tggcgaggag gatggggtcg
    49501 acaacagcgg tgtagagaac gcaaggctgt atacccaacc ggagcatgtg gcacaggcct
    49561 atgaagaaat gtcaaaaatc agccccttat ttaccgtagc cgcagccttt ggcaatgtac
    49621 acggggttta tgccccgggt aatgtggagc tgaaaccggt tattttaaaa aattcccagg
    49681 actatatccg ggagcagtat aaaaccaatt ccgggcaacc ggtcaatttt gtgttccatg
    49741 gcggctccgg ttgttccagg gaggaaatcc gcgaagcgat cggttatgga gcggtaaaaa
    49801 tgaacattga taccgatatg cagtgggcct tctgggaagg gatcaaagat tattacgaac
    49861 agaaaaagga ctatctgcaa gcccagttgg gtaacccgga aggggcagaa aagccgaaca
    49921 agagccatta tgaccctcga aaatggcttc gggccggaga ggagaatttc aaaaaaaggc
    49981 tgaaatccgc tttcgaagat ctgaactgca tcaaccggaa ctgatcaatg caaggcggcc
    50041 ctcaagggta atgaggcgat aatccggcct tcctgttcag tccaccagcg gagccggtca
    50101 tagacttctt tttcatcgaa gtattgtaaa gccatcttta cataaatatt accatgtttg
    50161 gcctctgagg tccagagcat cttataaaac cgtttgagtt cagggtcctc cagagcctct
    50221 tccaccagcc gaaatcgctc tgcgccccgc atttccacca gggaggccac caggaggcgg
    50281 tccaggaaac gcggcaggac cggggttttc atgagggcca tcaaggcccg gatgtaggga
    50341 tcttcgggca tggaggccgg gagttgcaca ccgcgcatct ccatgatctg atatacgagc
    50401 ttaaaatgtt ccagttcttc cagcgcagta tcgatgaggt cgggcaggat cgcagtccgg
    50461 tcggggtatt tggccaccat gctcagcgcc atggcgctgg ctttccgttc gcaatccgca
    50521 tggtccctta aaaaagcatc aaaatcatcc attacggcct ttatccatcc tggaggactg
    50581 gcttccgcga tatccaggct gagtttcatg tgctctggaa caagaggtag atgagcagga
    50641 acaggaccag ggtgatcacg atcacgacgg tgaaaaaatt tttcatttgc ctggcatcct
    50701 ttttaacgaa ggatttttta gtacccgatt ttttcatata aaataagcgg atttatattc
    50761 ccaaatctaa tgaactgcct tcaattccgg gccgggtcca tggtttatat tcagcaagtt
    50821 taattttccg gatggactca taaaactcgg tcactctttg gtccaccagg atgtccgcag
    50881 gcatgagagg cctgctcaat tgtatgcctt cgagcgtcct gcacctggac agggccacat
    50941 aggtctggcc aaactcgaag gccccccggc ccaggtcgat ccggatccgg tcaaaggtct
    51001 ttccctggct tttgtgtatg gtaatggccc aggccaggcg gatggggtat tgcctgaaga
    51061 ggccggtcac ttcggcttcg agttcctttt ctttattgag gcggtagcgt accatttgcc
    51121 attccagggg agcgaggtca aggatccggg tccctgcatc cttttcaatg caaaccctga
    51181 tccggtcgtg ttcaaggctc gtcacctttc cgatactgcc attcacaaat tctttggcgg
    51241 ggtcattctt tacaaacatc acctgcgccc cttccttcag caccaacaga gggtctgtcg
    51301 gatagctgtt ggcattaaat tctccctcca cgacagcctg gtactgatag gatttgcctt
    51361 caagcgccat taacctggaa gtgttgatct ggtccgcgat ccggttggtt gaagacaggg
    51421 tgatcacaaa atcactggtc agctcatggg tcccggctct ttcattgatc tggtcaaggt
    51481 catccggatc aaattcatgc gtacgcaccc ggtccaggag ctggataaac cggaattcag
    51541 cctggcggta aacatgatgc aactcgatga gccttacctc aaaaccctcc ttgaatacct
    51601 tggcggaata aaaatagggc gtgtcgtaaa tctggctcag gtggtcgcgt tcggcctggg
    51661 tggccaggac cggaggaagt tggtacagat ctccgaaaaa taccagctgg acgcctccga
    51721 agggctcgtc ctgtccgcgg ttgaccctta gaaacaggtc gatctgatcg agcaggtccg
    51781 ccctcaccat ggaaatttca tcgatgatca tcatttccat gcgtttgtac aactggtaat
    51841 tggagagccg gtgaatttgt ttccgaccga gggggtatgg aggaaaactg aaaaaactat
    51901 ggatggtttg tcctcttaca tttaaggctg cgatcccggt aggggccaat acgaccactt
    51961 ttttccgggt cgtgtcctta aataactgca acagggtgga tttgcccgtg ccggctttcc
    52021 ccgtgatgaa atacgatcca tccgtatttt ccaataaagc aagacattga cgaaagtcgg
    52081 atgtcagcgt taatggttcg aacctggagg aggacaaatc gcggacaata ttttttcaaa
    52141 tatatgcata taataagcgc ttaactaaga tctttgcgcg ccggtatggc ccggatgatt
    52201 atggaggaaa caaaaccttt ctttttcaaa cagtttaagg ttgcccagga caaagtagtc
    52261 atgaaagtcg ggacggacgg ggtattgctt ggcgcctgga tcgatgtcac cggtgctgcg
    52321 accatcctgg atgtggggac aggatcaggt cttatcgccc tccagcttgc ccagcgcctt
    52381 gagggcaagg ctgatattca cggcatagat attgacccgg cagcttgcga gcaggccagg
    52441 gaaaacttta cggcctcccc ctgggggggc tgcatgaccg tgattcagga ttccatacag
    52501 gactactcgg gcagggaatc aaaaaaatat gaactgatcg tcagcaaccc gccttttttc
    52561 accggggggc tcctttccct gaatccggat aaaaacgatg cgcggcatac gacaaaactc
    52621 cctcacggag accttctcag agcggtcagc agattactga agccggcagg gcggcttggc
    52681 ctgatcttgc cttatttgca gggactgagg tttatagaaa tatcagagtc ctatagctta
    52741 ttccctttga ggatcaccga ggtgaggacc cgggaagata agccggtcga acggatgctg
    52801 attgaattta cttatgataa aagggttact ccgcagggaa ataccctggt catccatcag
    52861 ggtgaaaacg actttacccc tcaatacaag gacctcaccc gggctttcta ccttaatttt
    52921 taaaaaacgg cgatgagttt cagtccaatc ccctggatct ttaacagcaa tatttatgag
    52981 gtaaacctga ggcaatacac gacttccggc agcctgaggg cctttaccgg ccatctccgg
    53041 agacttcgtg acatgggtat tgagatcctc tggttcatgc ccctgacgcc catttcgata
    53101 aaagcgagaa aaggcacctt aggcagttat tatgcagcct cggacttcgc gaccattaat
    53161 ccagaattcg gctcggaaca ggatttccgt gaactggtcg accaggctca ccggctgggt
    53221 ttgaaagtca tcatagactg ggtggccaat cataccgggt gggaccatac ctggacccgc
    53281 agccacccgg aattttacct gaaaaacgat gccggtcagt tttttgaaaa aaacggatgg
    53341 gaggatgtca tcgacctgga ttataactcc aaggccatgc gtcaagccat gacggaagcc
    53401 atggccggct gggtcagacg atttaacatt gacggattcc gttgcgacat ggccatgctg
    53461 gttcccctgg atttttgggt agaaacccgt aatgccctgg acagaataaa acccctggtc
    53521 tggctcgctg aatgtgaaga ccccgcctat tatgccgcct ttgataccat gtacggctgg
    53581 gaatggatgc atgccacctc ggcggtttac cggggacaac acgataagtt tcacctggaa
    53641 catattttat tcaaataccg ggaccggatg ccccggggca ggaggatttg cctgtttacc
    53701 agcaatcacg acgaaaacag ctggaacggt actgaatttg aacgttatgg cgatagcgcc
    53761 ctccttttcg cggcactgaa tatcatcctt ccgctgagca taccccttat ttattccggg
    53821 caggaattgc ccctcaggca ccgcctgaaa ttttttgata aagatgaaat tccatggtca
    53881 ggctcacccg acctggctga tttttacgat ggtctgcttg agattcgtaa aaccaatccc
    53941 ctttacggac ttcccgaagt ggctctttcc ttgaataaac tttgtgatga tccccgcatt
    54001 ttatccctgc aggcaggaat tcccggacaa agggatgttg caggaatatt taacctaagc
    54061 ccgatggaag tgagttcaca gttgaacgga tatcatttac cgggtgttta taagaacatc
    54121 ctcacccgcg aatcctttaa ccccgcttca aaccacccct tcaaactttc cccctgggaa
    54181 ttcagattgt ttgaaaacac cagtgtgtga cccgaacttt tccttaattc gacatcgcgt
    54241 gcgtctggct gatcagctct tttgcttttt gaagaatagt gctgtcctcc aattccacta
    54301 tgccgccgtt ggggccgggt tgaatatgtt gatccactat tttaccatca acaaatttct
    54361 tggctccaaa ataatttctt acagtctgct cgtcaatatt taaatcatgc gcaattttgg
    54421 aaacaccgcc agtgggcaga ctgtgtttga tttgtctcag ttcttcaaaa gtgatatgca
    54481 tattcaagtg gttttggatt aggaaaatac tttgaacacc tcaatttaaa ttctttccat
    54541 ccgaaatgca agaaaatgat aatatattaa caataaattg cgccccatta ccctgccctg
    54601 atctgatcga agccggacgc aaagttcagg cttcaagtac cgcctcaaca ggctggtaag
    54661 ggaggtcgaa cgcctccgca acgccctggt atacaacctt tccgtccaca atgttgagcc
    54721 ctttgcgcag cccgggattt tcgttgcagg cctttttcca tcccttgcct gccagttcca
    54781 gggcataggg cagggtagcg ttggtcagcg cgatggtaga ggtgtagggc accgcaccgg
    54841 gcatatttgc cacacaatag tggacaatat ggtcaatgat aaacaccggg tcggcatgag
    54901 tggtcggccg gcaggtttca atacatccac cctggtcaac cgccacatcg accaatacgg
    54961 tgcccggacg catggacttg agcatttccc tggtgatcag gtggggcgct ttggctccgg
    55021 ggatcagcac ggcgcccacg atgaggtcgt ggttcccgat tagcctgcgg atattgtatt
    55081 cgttgctgta catcgtgacc acgtttgggg gcaggatatc ggcgagatgc cgcaaccggt
    55141 tgaggtttac gtccaggatg gtcaccatag ctccgagccc cgctgccatt tttgccgcct
    55201 gagtacctac aatcccccca cccaggatca acactttgcc gggcggaaca ccgggaactc
    55261 cgccaagcag gatcccacgg ccctgcgagg gcctttccag gtatttggcg ccctgctgga
    55321 tagccatccg gccggccact tctgacatgg gtaccaggag gggcagggaa cggtccggcg
    55381 cttccaccgt ttcgtaggcc aggcaaaccg ctttattgct gatcatcgcg cgggtgagcg
    55441 cttcactgga ggcaaaatga aaataagtga acaccagctg cccggcccgg atcaggtcat
    55501 actcagactc gatcggctct tttaccttga gaatcatatc agagcttcga taggtatctt
    55561 caatgccgga caagacctta gctccggctt tgaaatagtc ttcatctgaa aacccgctgc
    55621 cttcaccggc ggtggactgg atctggacgg tgtggcccct tttactcagt tccatgacac
    55681 ccgccggggt cacagctacc cggttttcgt tacttttaat ttctttcgga actcctatga
    55741 tcataaggta atggtatttg gtcagttgaa tggtcggggc caaagatagt ttgttttgtt
    55801 ttaaaaaaaa caggcccctg atcgctcaga ggcctgtatc tggtactcgg atgattcgtt
    55861 tcttgtcaat gggtacccat caggatcatc ttccgggttg ctctttctgc agcggtttca
    55921 atggtataat aataaacccc ggatccgcca acaacccctt tgtccagcct gatctggttg
    55981 aaccctttta tcccgttcat tttctgtaca tggatggttt tgccggtcag atcggtcacc
    56041 ctgagagtga tggtttcttc cctgggcagg tcaaaggaaa tcagggtata ggtttggaac
    56101 ggattgggta cattctggta aagggagaaa tgcgcttcgt tttcctggtt gccaaaaacg
    56161 agtcgtactt cgcccgtttc ctttccatta aaggattctg aacgggtgta catgctgttg
    56221 atggcaaggt tatcgctgag ccgcaagggc ttcagggcgc gcacccgaag agtaaacagg
    56281 ggatggcctg cggcgatggt ctgtccggaa acatggtccc aactggcggt tagataatta
    56341 ttccggatat gaccaatatg ctgctgatct atggacaggg agccgccctg gatgtccagg
    56401 atttgcaggc cctcatgttt caggctgaac tggaaagcct cgatatccct gaaattgggc
    56461 gaagtgaagg tgatggttgc ttcttcgccg gaggtaaggg tcagatcggt tgtctggaag
    56521 gtgaggggtt tgacggccga cctgatttcc atccccaaaa ggctgtgggc ttgaacggta
    56581 ttgttcaggt ccccgatttt aatgccggtg aagtcacggt tcaattcgtc agaggaaagg
    56641 cctttgatat caatcatttc cgggaattca tagggcttgg ccatattgaa cttgaaggat
    56701 ttgggcacaa acctccagct tttgtttgca ggaaaatcat tgtacaggaa caggaccagt
    56761 ttcctgagtt caatgaggtc cagcgccgtg atttcccggt cgttgttgac atccgcggca
    56821 accatgtcgt acccggtgct gaaaggttga acccccagga tgtgtttttg aatcatcagc
    56881 aggtcaatgg tgctcacccc gttggaggct tggtcgttcc tggtgggctt cagtatatat
    56941 tgactgtgca tcggcaggtt cacaaaggca tattgaccgc tgaccgaggt cttgaacgcg
    57001 gggatgggca gattcgactg ggcctccacc aggacgtgct cgaccggttc attctgctgg
    57061 gtccggacca ttccggatat gctcccggca acactggccg cgtcatcaca aacatccccg
    57121 gcgccatcct ggatgctgat aaaggtcgca caatagtcaa aattgcctgc ttcatcccat
    57181 acatagatat cgatctgtac cgtaaaactg atcccgtttg gaatgtcttc gcatttgaag
    57241 actttgcagc tgtccgcgat gctgggtcca aaactgaact tgaggttttc ccttgcagta
    57301 caattgtcat aactccctgc gtctagatct ttggcccaaa ccttgatctc cccggtagtg
    57361 ggca
//
LOCUS       DAOQXT010000018        56585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_589_length_56585_cov_5.795206, whole genome shotgun sequence.
ACCESSION   DAOQXT010000018 DAOQXT010000000
VERSION     DAOQXT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 56585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56585
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_56585_cov_5.795206"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1070
                     /locus_tag="PKM27_04760"
     CDS             135..1070
                     /locus_tag="PKM27_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR06605.1"
                     /translation="MKKVLITGAAGFLGSHLCDRFIREGYSVIGMDNLVTGSLGNIEH
                     LFKLKEFEFYHHDVSKFVYVPGPLDYILHFASPASPIDYLRMPIQTLKVGSLGTHNLL
                     GLARQKKSRILIASTSEVYGDPLEHPQREEYWGNVNPIGPRGVYDEAKRFQEAITMAY
                     HHYHGLETRIARIFNIYGPRMRVEDGRALPAFFSQAIRGEDLTVFGDGSQTRSFCYVD
                     DLVEGIYHLLLSDYHLPVNLGNPDEITLMEAATEVLQLVGNSSQIVFKPLPVDDPKTR
                     QPDISRARSILGWEPKIGRREGFKHTLEYFKKVLK"
     gene            1070..2125
                     /gene="rfbB"
                     /locus_tag="PKM27_04765"
     CDS             1070..2125
                     /gene="rfbB"
                     /locus_tag="PKM27_04765"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNR06606.1"
                     /translation="MKSDKTILITGGAGFIGSHAVRHFINKYPYRIVNLDALTYAGNL
                     NNLKDIQGHPNYHFEKADIRDRDALDRIFNFYKPDWIMHFAAESHVDRSIMDPLAFIE
                     TNVLGTANLLLAARKHWQELQGKLFYHVSTDEVFGSLGAEGYFSEDTPYDPRSPYSAS
                     KASSDHFVRAYHHTYHIPVIVSNCSNNYGPYQFPEKLIPVVIQNILNEKPIPVYGDGT
                     NIRDWLYVQDHIDAIDLIAHRGKTGETYLIGGHNELRNIDLVRLLCNIMDHKLGRTKG
                     HSENLIRFVKDRPGHDQRYAIDASKLEKNLGWLPMIKPEIGLEMTVDWYLKEKEWLAG
                     VTSGAYQAYYEQQYQNR"
     gene            2130..4835
                     /locus_tag="PKM27_04770"
     CDS             2130..4835
                     /locus_tag="PKM27_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNR06607.1"
                     /translation="MSRLFRYAFLFFIAWSLSVDAEAQIPAQAAPLQAELQRRGISEQ
                     ELQARLLQKGVDLNDLQNLTAEQALQFQAIIEETINELEREKTGRAVDTSKPAVRSAP
                     DTLASPKPDSTTPKAPDPPGTLKIYGQELFRNKSIQAYTSSENIKPPETYVLGVGDIV
                     NVHVFGRSKVDFKNLEINSQGYIQMANYPRIYIKGLTYQQAREVVRKTLNAYFTFQPN
                     EFEMTIDYSREISVNIFGEANQIGTFRLPATNTAINALVAAGGPNDIGSIRNIRLIRG
                     RTVKKIDVYEFIANPAVAQDLYLEENDIIHIPLAERVVAVEGAVRRPMRYELIAGENL
                     VKLIEYAGGLKDDAYRKNIQVRRFIDDKPQIIDVPLRTLLDEKSDFTLMSGDLVQIAS
                     ITRPLEEFVQIDGQVQTPGSYQFTDGMRVSDLLEKGNLLFGAKTDLAFLMRLNENGNY
                     SVNRIHIRAIQENKNGPENILLKARDRVMVYSLSYYLDSSFIYVSGAVRNPVRIPFDF
                     QRNLRVSDLLTMSGGLLPDALDFGHIRRINKENYVEKDYITFNSKTALARPGTAEDPV
                     LEPDDEILLYNDRTFNDASTISIDGAVRNPGSFAYGPGITLKDLVVLAGGFSLSAATN
                     RIEVFRVVMDDNQPTRTVVATLNMPRDITAPGHRPPDFKLQPFDQVQVRAVPQFEMQQ
                     NVSIEGEVQFPGTYSLITENETITELLRRAGGPTQEAFLPGVTLFRTQANAGYIIFSL
                     TDAIKSPSGPDNLVLKDGDRIVIPKVIDFVTIEGATDASELYQEKLLESNNRIHIAWV
                     PGKNARYYVNEFAAGVAREGSKSRITVEQPNGRIARTKNYWFFKVYPEVEKGAVVKVG
                     EKLSKQKTGDEERKKIDWMKVMADTLGQATAVLSFILLVRALDKQ"
     gene            complement(4849..8049)
                     /locus_tag="PKM27_04775"
     CDS             complement(4849..8049)
                     /locus_tag="PKM27_04775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNR06608.1"
                     /translation="MKKKSTTPSPDLFSMHEKKKLKDQFYKALSILNEGQREAVESID
                     GPVMVLAGPGTGKTQVLAARFGNILEKTDASIPQILCLTYSEAGVQAMRDRLFQFIGP
                     AAHEAHLFTYHAFCNKVIQENPEYFFDYKKFRLASDLQRFELIEKILLELPDDHVLQR
                     LIGDRHWDIQRLAKQYESIKKEQRDLPKLIQGIREHIAGMDSDPDYRYKRGGKSGNKG
                     EFKPAYFQDKARYERAIDALKTFDRYAEKMAEQQWMDFNDLLLQVQQAFKEHPDLLAR
                     YQENYQYFLVDEFQDTNVIQNNVLLSLCEYWDQPNLFVVGDDDQAIYRFQGADQQNLV
                     TILRKYPDIRIICITENYRSTQSLLDAADAIIEPLEERIHKLELEIPPQIRMKINKKL
                     LSTRGSAEDRPPELRVYQNPQQETLALYDHIRKIWETNPGSLGEHAVIFRRNKTVQDL
                     VYLLELAGIPINFRSSRNLLEEPLIRHLILLLEYLQAEFLQKNSGEPFLIQLLYLPYW
                     GLDPHDVSTLSWLERKTRDNKNLAGKILDPESWPEAGFKNRPALAAFIGSTREWIQSL
                     PPVQTLSLITEDILRKSGMLSWALNSVDRNWHLRAINSFLAMIRNEVQENPAMDLSAL
                     LDRIHKMENFGIYLPLESQYAAEQGINLLTAHGSKGKEFDSVWIMNALAQDWEDYFLG
                     SEQPYKIPEGLKQLDQSKASQKHDERRLFYVSMTRAKKSLVVSWSLAGIDQKPMRESV
                     FASELRQFLKSDPPVQQVDENLLIEKFQLIQNKKSTPPELLDHDLISQFCTDYVMSCT
                     DLDKYLECPRRFYFEEILKTPQAPNAHIGLGIAVHEALFDFFNAALKAPGLQADYLVD
                     FFLTRMKKHEWYFTPDEYEGLLRRFTHQLPLFYKQHQALWEGCRDFRLEYYLDRTLFE
                     QVPIHGKLDQVLVKEGSARIIDFKSGRGDSTSSKDKLKMPWRGSMGGNYWRQACFYKL
                     LLDRDKNKTWNATSAQVIFLQVNKLDQEGFLNKEYLFSQEDLDIVGNQILDTYQKIQN
                     HEFDLGCDKPECEWCSFIRHRDVILRRADSDRKAEE"
     gene            complement(8046..9338)
                     /locus_tag="PKM27_04780"
     CDS             complement(8046..9338)
                     /locus_tag="PKM27_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR06609.1"
                     /translation="MKKSYFCGMTGLRAAFLVFFAGLLTTGCFRGSYAGEKSRLNGLL
                     YSSPLVKKVLDSFDRYEAQIIYTQIDRDRANRPHFKSLRWNYRPLHYFYPASVVKLPV
                     ALLAAEKIKRLQAGHPALDLFTAMRADSARPPQSAFSEDASAWNGQASVGHFIKKIFL
                     VSDNDAFNRLYEFTGQAYIHERLRSLGFHKTHIIHRLSAPEFDLDANRYTNPVHFFEE
                     DLRIYDQAEQYNPAEVRVPDLGSLLKGQAHYAGDSLVHAPFDFSAKNFFSLGDMEQMI
                     RAIVFHEYMPEGLRFDLDPDQRRYLLKCMSQLPSESRFPRYDSTYHDGFCKFFMYGDS
                     KERIPDHLRIFNKVGWAYGYLTDVAYIVDFKNKIEFILAATVNANTDGVVNDGLYDYE
                     NLGLPYLSRLGKLFYSYEQSRERRHAPDLSSLIFDYRE"
     gene            complement(9359..10111)
                     /locus_tag="PKM27_04785"
     CDS             complement(9359..10111)
                     /locus_tag="PKM27_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNR06610.1"
                     /translation="MGFSEFILVIGGMLAGVINTLAGNGSVITLSLCTELIGLSPSMA
                     NGTNRLGILTQSIAGTWSLHRSGKLLLAGTGRAIFLVTLGALTGIWIATRVNDDQFRQ
                     LYPWFLVFIFIVLVLKPERWLKADLHEESREAWYLFPVYFLIGVYGGFIQIGVGVFFL
                     AATIMLARKPWKNANAAKILVTGIFTLFAVFLFHSRGLFDLKTALFLALGQTFGAWLG
                     VKYLNHYAHAHVASYYILLVITALTIVRQFFF"
     gene            complement(10111..10515)
                     /gene="mce"
                     /locus_tag="PKM27_04790"
     CDS             complement(10111..10515)
                     /gene="mce"
                     /locus_tag="PKM27_04790"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNR06611.1"
                     /translation="MLKLDHIGIAVKNLALAREVYSTLMNEEPYKKETVPEEKVTTLF
                     FKAGDTKIELLESLDQDGPIRKFIEKKGEGIHHIAFEVPDIHEAVRDLKKKGFRFVDE
                     NPKKGADRKLIVFLYPKNTHGVLIELCQSLPE"
     gene            10601..11374
                     /locus_tag="PKM27_04795"
     CDS             10601..11374
                     /locus_tag="PKM27_04795"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNR06612.1"
                     /translation="MKSKKEIVDNWLPRYTGVPLERFGEYIILVNFNNYVELFAQWNG
                     VPVEGGDRPMDSATAGNISIINFGMGSPNAATIMDLLSSIKPKACLFLGKCGGLRPEK
                     TVVGDLILPIAAIRGEGTSNDYFPPEVPALPAFALQKAISTTIREYQRDYWTGTVYTT
                     NRRVWEYDEHFKDYLKSLRCMGIDMETATIFITAFKNQIPAGALLLVSDVPMVPEGVK
                     TEEGDKKVTADYLQTHLKIGIDSLKQLINNAQTVRHLRF"
     gene            complement(11379..12167)
                     /locus_tag="PKM27_04800"
     CDS             complement(11379..12167)
                     /locus_tag="PKM27_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR06613.1"
                     /translation="MDLSLINRNALVCGSSKGIGKAIAVELASLGANVTLLARSRDLM
                     QEALHALPRIHPGQDHDYLMADYEDRADLHKKVNALTSARHFHILVNNTGGPPTGQLL
                     DFPVDKIEESFRKLFLINHELVQLVVPGMKSEGYGRIINITSTSVKEPLADLGLSNSL
                     RAAVSNWAKTLANELGPYQITVNNVMPGYTLTERLQEIFDFRAKKLNATRESLEQKAI
                     EEIPLKRFGQPREIAAAVAFLASPAASYINGINLAVDGGRTKSL"
     gene            12248..13516
                     /gene="kynU"
                     /locus_tag="PKM27_04805"
     CDS             12248..13516
                     /gene="kynU"
                     /locus_tag="PKM27_04805"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNR06614.1"
                     /translation="MEPASLEYALAKDEQDPLGSFRERYALPVNRDGDPSIYFCGHSL
                     GLQAFQSNYYVKEEMDRWARLGVKGHFDGEPAWVDYQDKLQQILSGLTGANPGEVVIM
                     NTLTVNIHLMLVSFYRPTRDRYKVLVEYSPFPSDQYAIKSQIRYHGFAVEDALIELKP
                     RTGEYKVRMEDIEEIIDLHGPSLALVYFGSVNYLNGQAYSLPSLVQKAHAAGAFFGLD
                     LAHGAGNLLLKLHEWEVDFAVWCTYKYLNGGPNSLAGCFIHQKHHAGTPIPRFEGWYG
                     HDLQRRFIMEPDFIPMPGAAAWQLSGPPILSLAGMRAGLEDFEEAGMERLRNKSVQLT
                     AYLGSLLDSLQDPRLVIITPADPEMRGCQLSLRLDPPDKRIFHRLMDSGVVVDWREPG
                     IIRVAPVPMYNSFRDVYAFYVILKQILKDL"
     gene            13513..14289
                     /locus_tag="PKM27_04810"
     CDS             13513..14289
                     /locus_tag="PKM27_04810"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNR06615.1"
                     /translation="MRNKIFFCSDFHLGLDAAETSSVREKMILEWMEKNEPEMEALFI
                     LGDIFDFWFEYKQVVPRGGIRIMAALARLRDRGIPVQVFTGNHDQWMFGYFQEELDIP
                     VHHDPVSFTLQNKKVMLGHGDGLGPGDHGYKFIKKVFRHPVARTLFGWIPPGMGIPLA
                     RYWSGASRRKGAREHAFLGPENEWLIQYCEKTVHKEPGNTDYFIFGHRHLPVYWRLGD
                     GSHYINLGDWLRYHSYAVMEEGIIRLDFYRQEGSKPISNF"
     gene            14300..15241
                     /locus_tag="PKM27_04815"
     CDS             14300..15241
                     /locus_tag="PKM27_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR06616.1"
                     /translation="MKTPAVSFLFFLACTSVILVQPACRHATPGPDPLAALYSNTQVI
                     GDIVYKTAGPHSLKLDAYIPVTRLGEPPWVEYSPDLKPVLFFLHGGGWTSGNKISRSL
                     FLMPFLSKGFCVVTADYRLLSQASLPEIISDTRAALRWIYDHSRQYKFDTARIVVSGE
                     SAGGHLALMNGVLNESSRFPPDSNEAGHELKVAAVVNWFGVADLVKASSDWDSVYLSQ
                     VAGPDPAKTTTVLRSCSPVNYVDAHTVPILTIHGSEDRAANYDQALLLHDILGKKGIK
                     NQLLTIPGKKHGNFNSREMDQAMQEIWNFLSSIQILN"
     gene            15283..16077
                     /locus_tag="PKM27_04820"
     CDS             15283..16077
                     /locus_tag="PKM27_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06617.1"
                     /translation="MVWLYQPAFGQDTLPDSFSLVTRVPLEATAVVTDFLGNIYVISR
                     QNELVHLDERGLVLGRYSNNYLGRPQLIHTNSPLQLVLFYPDFQTLLILDKTLNELKR
                     IRLADLGLPYMSALGYSSDRELWYFDEPVRRFRRINIQGKLILESSMTFVPFEGEVVK
                     IRIQNNEVGAWTNAGRIFILDLNGHLKRQIDLEGEWISWKEDEMVFFYNQTIRNHSPK
                     GLSGATDLSGLDGQPYTSLGLLPAGIVATNGKEVYIFRSVPRKERN"
     gene            complement(16051..16470)
                     /gene="rnpA"
                     /locus_tag="PKM27_04825"
     CDS             complement(16051..16470)
                     /gene="rnpA"
                     /locus_tag="PKM27_04825"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNR06618.1"
                     /translation="MAPEEIRSGSNRFPPREKLKSRKSIQVLFDQGKSFSIPPLRIIF
                     LIHPTEEAKNQGVRFAISVPKKIAPLATRRNRIKRLIREAYRLGKTPLIEACRSKGIL
                     LEMMWIFNGNPEKTDFGLLRPVAAKAINKLISFFSRN"
     gene            complement(16479..16634)
                     /gene="rpmH"
                     /locus_tag="PKM27_04830"
     CDS             complement(16479..16634)
                     /gene="rpmH"
                     /locus_tag="PKM27_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNR06619.1"
                     /translation="MKQTFQPSRRRRSNKHGFRARMSSKSGRKVLSNRRAKGRHKLTV
                     ADEARIK"
     gene            complement(16714..18534)
                     /gene="rho"
                     /locus_tag="PKM27_04835"
     CDS             complement(16714..18534)
                     /gene="rho"
                     /locus_tag="PKM27_04835"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNR06620.1"
                     /translation="MIYDILRLNEMLVPELREIADQLGIKGNRKLNKQDLVFQILDRQ
                     AMGGAAASSMETGPSTESEAQDTAPRKEGAHQKQNGHHDSKPQRPRMRKAIQEDAEPV
                     NIGNREPMEQREQREQREPREQREQREQREPREQREPREFREPREQREHREQREPRES
                     RRPHHAQPAHGKPHAPAAMKTSAPTSAPIPSPAPTPSPAPRPTPVVNDTPAMPNGVAE
                     PELEAAIPAEIFNVDLDAMIEGQGVLEMMPDGYGFLRSADYNYLSSPDDIYVSPSQIK
                     LFGLKTGDSVIGAIRPPKEGEKYFALLKVSLINGLTSEQIRDRVPFDYLTPLFPTKKF
                     KLTTHPAGKDYGNRLIDLFTPIGMGQRGLIVAQPKTGKTFLLKDIANAISYNHPDCYL
                     IILLIDERPEEVTDMQRSVNAEVISSTFDEPADNHVRVANIVLEKAKRMVECGHNVVI
                     LLDSITRLARAYNTVAPSSGKVLSGGVEANALQKPKKFFGAARAIENGGSLTILATAL
                     IDTGSKMDGVIFEEFKGTGNMELQLDRNLSNKRLYPSIDVTKSSTRREELLMDKDSLQ
                     RMFILRNHLADMKPDEAIEFMLKHMQHTMNNEEFLQTMNS"
     gene            18729..20033
                     /gene="serS"
                     /locus_tag="PKM27_04840"
     CDS             18729..20033
                     /gene="serS"
                     /locus_tag="PKM27_04840"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNR06621.1"
                     /translation="MNFFSPHLMLTFNYFTNLKDKTIAGLKKRNWDEQKLQWVDEIAL
                     LDSERKELQTALDQLKMQVNQASRQIGNLMKEGRHQEAETTKNQVAGHKSEITSLEEK
                     FRTNKERIQELLLKIPNIPHESVPPGASPEDNEVFKAWKAPLPELFPGALSHWDLAEK
                     YQLFDLKLGVKITGAGFPLYRGKGAKLQRALIQFFLDQATAAGYEEIIPPLLVNADTA
                     MATGQLPDKDGQMYYCERDQLYLIPTAEVPVTNVYRDEILEESALPIKLCGYTPCFRR
                     EAGSYGADVKGLNRVHQFDKVEIVRLEHPDHSYQALDKMLEHVSGLLDRLELPYRILK
                     LCGGDMGFTSALTYDFEIFSAAQKKWLEVSSVSNFETFQSNRMKLRFRNAEGKTQLLH
                     TLNGSALALARIVAGLLENHQTPDGIRIPAALQPYTGFSRID"
     gene            20071..20775
                     /locus_tag="PKM27_04845"
     CDS             20071..20775
                     /locus_tag="PKM27_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNR06622.1"
                     /translation="MNMYSSGYFLLSIGLAAVGFVVQQILLAKMRKYGAAPVSRGMSG
                     KEIAETMLNYYNIRGVQVVPGQGMLTDHYNPLTKTVSLSPDVYEGRNIMAAAVAAHEC
                     GHAVQHATAYSMLQLRSKLVPVVQVASFGLQWMMLLSFMLANSFPQLMLVTIGLFALT
                     TIFSIITLPVEFDASKRALAWLNAQAVLRSGPESEGAKDALKWAAMTYVVAALSSIVM
                     LVYLILRYMGLSRSDD"
     gene            20797..21363
                     /gene="frr"
                     /locus_tag="PKM27_04850"
     CDS             20797..21363
                     /gene="frr"
                     /locus_tag="PKM27_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNR06623.1"
                     /translation="MMSAELEKHMASVQDQMSKAVEHLQHELLKVRTGKANPAMVHEL
                     RVNYYGNPTPLNQVANVGLSDSRTITIQPWEKSMLAPIEQAVFAANLGLTPMNDGEMI
                     RINVPPLTEERRKDLVKQAKHMGEEARVAVRNIRHKYLDVIKKAVKDGTPEDEGKKKE
                     AQLLELVNQHVEKVDKLIKSKEDEIMKV"
     gene            21417..22244
                     /locus_tag="PKM27_04855"
     CDS             21417..22244
                     /locus_tag="PKM27_04855"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNR06624.1"
                     /translation="MHRLEQIEEAVRFVRTRTELIPKVGIILGTGLSGFSDELTGRQE
                     IPYRRIPHFVESTVKGHAGKLVFGLLENVPVVMMAGRFHYYEGYSLDEVTFPVRLLRY
                     LGVERMIITNVSGALRPEYRAGDILAVRDHINLIPSNPLRGPNDERIGPRFPDMVNTY
                     DEKILEKAGEAARTLNLTLHTGVYISVPGPSLETKAERIFFQHIGADVVGMSTVPEVI
                     VCKHMNLPVNVFSIISNEFNVETSAEEVISVAQAYEGRLRNLIKSILAGVNSDTAGN"
     gene            22311..22703
                     /locus_tag="PKM27_04860"
     CDS             22311..22703
                     /locus_tag="PKM27_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNR06625.1"
                     /translation="MILIMLEISVPGGFIMLFFGLGALTLSVLTFFIDMGWMFQIALF
                     LVSSLMYLFLLRENAQRFLNPQIKEDPENEFIGKTAKALTPISDMEGKVEFKGTQWTA
                     LSKEPIPPGTEVEIVGQESITLFVKPIN"
     gene            22711..23658
                     /locus_tag="PKM27_04865"
     CDS             22711..23658
                     /locus_tag="PKM27_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNR06626.1"
                     /translation="MFTAGSIAYIIFAAFALLVLSSAVIIVPQRKAYIVERLGKYLGT
                     LAPGLHFLIPFVDKVRYRHSLKEQALDVNPQVCITKDNVAVEVDGVLYFQVMDPTKAS
                     YGIEDYRFASIQIAQTTMRSIVGRLELDKTFEERETINASIVEAVDKASEPWGVKITR
                     YEVKNITPPQSIRDAMEKQMRAEREKRAMIAESEGTMQAKINVAEGEKQEAIKKSEGE
                     KLKRINEADGRAAEIEALAIATANGLRTIASAIDTPNGKDAVSLRIAEQYLNEFGKLA
                     QKNNTMILPANLMDITGVLATAKKVLDHTQPADNGKAKV"
     gene            complement(23911..24519)
                     /locus_tag="PKM27_04870"
     CDS             complement(23911..24519)
                     /locus_tag="PKM27_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06627.1"
                     /translation="MSKEIPETGSVPKPNGKSRYSDKELAEFKALIDEKLLNARQELN
                     YLQEQIVEMNENNADIQGSDWFDDSSIHSEVELLNNMAIRQRQFIQNLENALVRIENK
                     TYGICTVTGNLIDKKRLLLVPHATKSIEAKHIEQASMVPDINQVAPDERVIRRSLEGK
                     QLKKSGVKRPKSKASKDFEDEDAEEIAESPIEDVDMNYEGGD"
     gene            24695..24913
                     /locus_tag="PKM27_04875"
     CDS             24695..24913
                     /locus_tag="PKM27_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06628.1"
                     /translation="MAVAAFAGVDLLVMVFVLLPALVAVAFTTLAGAGFKALDGLAFS
                     FFFAEAAPEDFVPFFALAFVLDVVAINN"
     gene            25141..28668
                     /gene="dnaE"
                     /locus_tag="PKM27_04880"
     CDS             25141..28668
                     /gene="dnaE"
                     /locus_tag="PKM27_04880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNR06629.1"
                     /translation="MPDLIHLHCHTQYSLLDGACGIPDLIKLAKNDGQQGIAITDHGN
                     MFGAFSFVHEANKAGIKAIVGCEFYLVEDRRKQVFSKIKGEKDERYHQLLLAKNQRGY
                     LNISKLCSLGFIEGSYGKFPRIDKELILQYHEGLIATSCCLGAEIPQAILAGKLELAE
                     ELVKWWKNVFGKDFYIELQRHGLENLDNSGLSQEDVNRVLIQLAKKYNIKTIATNDVH
                     YLTREDWEPHDILLCVNTNSKKTETERFRFPNDQFYFKSREEMGRLFADIPQALEHTL
                     EIFSKVDKLDLARNILLPNFPIPAGYASQDEYLRHLVYEGARHCYPELSQLVLDRIEH
                     ELRIIFGMNFSGYFLIVQDFIKAARELGVRVGPGRGSAAGSVVAYCLGITGLDPIKYS
                     LLFERFLNPERVNMPDMDIDFDDEGRQKVIDYVVKKYGRDQVAQIVTFNTMAARSSIR
                     DVGRVLDYPLSDTDRLAKLVPAKPKIYLADILNKPVDDLKAALTPDEINQVNQLRDIR
                     SGGDQASTVLALAERLEGSVRNTGIHAAGIIIAPEAIDRIMPVFTLKDTELLVTQYEG
                     TLMEDAGMLKMDFLGLRTLSIIKDALNLIEENTGTAVNIDQIPLDDPATLELFQRGDM
                     IGIFQFESDGMQKYLRDLKPESIEDIIAMSALYRPGPMDNIPSFIRRKRGQEQVEYPH
                     ERLEELLRPTYGILVYQEQIMQTAQIMSGYTLAAADLLRRAMGKKKHDEMQVHRKLFV
                     EGAIEQGIDETKAEEIFELMARFADYGFNRSHAAAYSILAFQTGWLKTHYPRQFMAAV
                     LTHNKQDISKLEYYLQEAKRLGIKVLGPDINESGLNFTVTRDQAIRFGLSALKGVGEG
                     PVEDILRERQAHGLFTNIFNLTSRLNSRTVNKKSLEALVLGGALDALHPEVDRSQYFA
                     PTDKYDSFLEYALRLGSQWQEMKSRNEMSLFSLESDTPLPDPVFPKIREPWSLVERLN
                     REKEVTGIFISAHPLDEYKYILDHFTTCTVANIPQYKDMPIRVAGIVTRSDHRISKKG
                     TGYGQFELRDFEGTFEFPLFSENYLDFKNRLELGQMVYIKGTYQVFNDGNSSRFRIQS
                     VQQLASAGPSIAENVLLQVPVHQLDETKIAKLKSICENQKGKSRLKIVLVDRASQTSL
                     QMVSEEYRIEVNADLLNQFKGLGLEYVLN"
     gene            28947..29420
                     /locus_tag="PKM27_04885"
     CDS             28947..29420
                     /locus_tag="PKM27_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNR06630.1"
                     /translation="MRKVGWIGLMIFCCFGISAQSESPRNLSLEEFKALCAGEAGKSV
                     ILNFWATWCGPCMEELPWFDRLKKEMPGVRIILVNLDFEKEVKSKVWPLLEKGYKQCE
                     VYRIQGLHPDDWMPLVEPAWSGALPTTLILNNGKRKFFEQKFEGYDSLKNEIVHF"
     gene            29436..30041
                     /locus_tag="PKM27_04890"
     CDS             29436..30041
                     /locus_tag="PKM27_04890"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR06631.1"
                     /translation="MKKSLYLVLAAACLSLFFTLPSPQGYQVGDVVADFSAKDVTGKM
                     ISTKDFPKAKGYVIVFTCNTCPYAKLYEDRIISLARKLDDAAWPLIAINSNDRNMSPG
                     DSFEKMQERAKSKAFPFPYVYDKSQSIVQAFGASKTPHFYILDASKKVKYIGAMDDYP
                     EDEAGVKTHYVQQAIQAIEKNQDPDPSFTRAIGCGIKKRTT"
     gene            complement(30064..30765)
                     /locus_tag="PKM27_04895"
     CDS             complement(30064..30765)
                     /locus_tag="PKM27_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HNR06632.1"
                     /translation="MVLVEICAQSYRAALAASENGAHRIELCTHLGGGGLTPSLGLIE
                     QVLEEVRIPVFVLIRPRTGDFYYSGAELSIMEKDIRHAVRAGAQGIVAGCLTADHQVD
                     VESMRRLVAAADGLPFTFHRAFESVPDSRQALETLIALGVHRILTGGRTGHAPNNRFE
                     LKALQQLAGDRLRILAGSGVSPDNVMELVRDSQVKEIHSSAKYDPAAMTSQSGDPLDS
                     DPAKVRALVACCTGL"
     gene            complement(31194..31598)
                     /gene="apaG"
                     /locus_tag="PKM27_04900"
     CDS             complement(31194..31598)
                     /gene="apaG"
                     /locus_tag="PKM27_04900"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNR06633.1"
                     /translation="MNKSSNTVQKTTLGVKVAVEVFFQPKYSIPFSDQNVFTYHISIA
                     NENPFAVQLLRRHWWIWESNGTRRQVRGEGVIGEQPVIQPGDAHRYESFCPIASDIGK
                     MHGIYQMIRLDNQEIFDIEIPEFQLMVPSVEN"
     gene            complement(31595..32161)
                     /gene="gmk"
                     /locus_tag="PKM27_04905"
     CDS             complement(31595..32161)
                     /gene="gmk"
                     /locus_tag="PKM27_04905"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNR06634.1"
                     /translation="MGKLIIITAPSGSGKSTIVNHLLQTFPRLALSVSATTRARRPYE
                     ADGREYYFITVSSFVQYLRQGRFVEWEEVYPGQFYGTLKSELKRLWALRKIIVFDIDV
                     WGAKDLKRMFPKNSLAIFIKPPSLGVLRQRLQARNTETPEKIAVRMARASMEMDFENQ
                     FDALVINDDLETAKKEIELLIEKFIKGK"
     gene            complement(32167..33036)
                     /locus_tag="PKM27_04910"
     CDS             complement(32167..33036)
                     /locus_tag="PKM27_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNR06635.1"
                     /translation="MLISMTGYGRSGGEIRNHKIRVEIKSVNSKFAEYRFRLPILFRD
                     KEFEIRKLLNGSIDRGKIDFLIEQADADLNEVQINSSLFVRYAEVLNQLSSQAGLGRE
                     PLIPSILSLPNVVESSESPLTDEEWAVAENIIKTAIEEFNAFRAHEGLILAGVIQTNV
                     VNIKQYLSQVESFEKGRTDQVRLRLNRMMTEFTNRPDYDANRFEQELMYYLEKIDFTE
                     EKIRLAKHCDYFIEKLNLPASNGRELNFISQEMGREINTLGSKAQEHNIQHLVVAMKD
                     ELEKIKEQLANVV"
     gene            33313..33774
                     /locus_tag="PKM27_04915"
     CDS             33313..33774
                     /locus_tag="PKM27_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR06636.1"
                     /translation="MPMYNLNINGKQHKAEVSDDTPLLWVLRDHLGLLGTKYACGIAT
                     CGACTVHLNGEAIRSCVLPVSSVGNAQVTTIEGLSANGEHPVQKAWDEVDVPQCGYCQ
                     AGQIMTAAAFLKKNPNPSDKEIVEAMNSNICRCGTYHRIKDAVKIAAKNMK"
     gene            33807..35972
                     /locus_tag="PKM27_04920"
     CDS             33807..35972
                     /locus_tag="PKM27_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNR06637.1"
                     /translation="MSTSKIASPNRRQFLKTTTLAGGGLFLSFDLFSKNSSSRMKVQS
                     GEADLVHFNSYLSIDPDGTVTIFSPNPEVGQGIKTSFPMIIAEELDLDWKKIKVVQAP
                     LDTKRFERQVAGGSRSTPHSWKRLREAGASARNLLLQAASAQWKVPMDQLSTSMGMIH
                     HKATGKMEHYGAFAGAAAKLDLPKEIPLKDPSQFHTIGTWVKGVDNMEVFTGKTQYGL
                     DIYKPGMKQAMIVRAPAFGLKLKSVNGEAAKSMPGIEEVVSFNNRVAIIGKSTWEIMK
                     ARKALNIEWQKDTDLESSEMHNKILSDMIGSGKMETRRAEGNIEQAFKDAVKTVESEF
                     QCPFIPHNSMEPMNFYADVREDSAELVGPTQNPESARNAVARLLNLSQDKVNVVFTRM
                     GGGFGRRLNSDFALEAAELSKLIKSPVKVTWSREDDMTGGIYRPACKYKFKAGLDANG
                     NMIAFQLRAAGINSGNPSRENNFPVGAIDHVLVESSDYKSPITTGPWRAPITNFLAGA
                     EQIFFDEVAEAAGKDPVALRLEWVAKAKAKPVGKVLYEPDRFKAVIDLVAEKSQWFKK
                     KKGVYKGFSVYFAHSTYVAQVAEVVKVKGKPVVKKVYCAVDCGILVNQSGALNQIVGS
                     VVDGMGAAMYGRLTFKEGSPNETNFDKYRMIRMGEIPDVEVHFVKNTIDPTGLGEPAL
                     PPVSGAIGNALYRATGIRYRDQPFIDYMNEGKAGAGQNM"
     gene            36252..36758
                     /locus_tag="PKM27_04925"
     CDS             36252..36758
                     /locus_tag="PKM27_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNR06638.1"
                     /translation="MERFKEYSIPVSGLKPGFHEYRFEMDPGFFSHFEKSPLADGRFK
                     AEVLMDRRQDLAVLDFRIEGSYACACDRCLAPIELPVHTEYRLILKFEEGKDEDDVIY
                     LDAQASEWNAAQLIYELIIMAMPLTNVYDCKGIPCNQDVLRQLEKTENAPGGDASVWE
                     DLKKIKLN"
     gene            36783..36971
                     /gene="rpmF"
                     /locus_tag="PKM27_04930"
     CDS             36783..36971
                     /gene="rpmF"
                     /locus_tag="PKM27_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNR06639.1"
                     /translation="MPNPKTRISRSRKNKRRTHHKAELPNVSVCKTTGEAFLRHRAYW
                     HEGDLYYKGRVLVKGATE"
     gene            36978..37358
                     /locus_tag="PKM27_04935"
     CDS             36978..37358
                     /locus_tag="PKM27_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNR06640.1"
                     /translation="MKQIVKTGEAPLPIGPYNQAVKANGVLYISGQIAINPADGSLVN
                     DSLEAETHQVLKNLGAILRAEGLDYEQVVKCTVFIKDMHQFSRINAVYGEYFPEATAP
                     ARETVEVSNLPRFMQVEISAIAVY"
     gene            37368..38390
                     /gene="trpS"
                     /locus_tag="PKM27_04940"
     CDS             37368..38390
                     /gene="trpS"
                     /locus_tag="PKM27_04940"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNR06641.1"
                     /translation="MKPENSEKERVLSGMQPTGNLHFGRYFGAVKNWVDLQAAYDCYY
                     CIVDYHAMTMPYKPANLRASTWNGIFGLIACGIEPRNLFIQSWVPEHAELTWILSCFA
                     SYGEVQRMTQFKDKSQQVAEKDKEAFISVGLFTYPVLQAADILIYKPKYVPVGKDQEQ
                     HLELTRNIAIRFNQQVGKEILPVPEALFTEIPKVMSTADPSRKMSASLGDKHNINVFA
                     DPDQIRKQIRTAVTDTGTETSSAMSPGVANLFSLLKASGQTETYAELKSRYEDQSLKY
                     AELKEVVAEALVRVSAPMREKMDHIQANKKEYKEMIRASSESIRKRAKHNLDQIKDAC
                     GLMITS"
     gene            38450..39061
                     /locus_tag="PKM27_04945"
     CDS             38450..39061
                     /locus_tag="PKM27_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNR06642.1"
                     /translation="MKIICVGRNYINHAKELNNPVPAEPVLFMKPSTALLINRRPFYF
                     PDFSKNIHHELELVIRISQNGKAIQPAFAHKYYDSVGLGIDFTARDLQEKCKEKGLPW
                     EIAKAFDYSAVLGDFIPLSELKNREAIEFELRKNEAPVQTGNSKDMIFSFDQIICHAS
                     RYFKLLKGDLIYTGTPAGVGPVAAGDTLTGYLEGQPMLYVSIK"
     gene            39155..40411
                     /gene="metK"
                     /locus_tag="PKM27_04950"
     CDS             39155..40411
                     /gene="metK"
                     /locus_tag="PKM27_04950"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNR06643.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDHFLAFDPESKVACETL
                     VTTGQVVLAGEVKSKTYLDVQDIAREVIRKIGYTKSEYMFEADSCGILSAIHEQSADI
                     NRGVDKKNREDQGAGDQGMMFGYACRETEDYMPLPLYLAHQVLLELAALRRENKQIKY
                     LRPDAKSQVTIEYNDQNQPVRIDTIVISTQHDPFADDQVMAEKIYKDMVGILIPRLKK
                     RLDLKNQKLFDQKIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKNLVAAGVCDEVLVQVSYAIGVAQPMGLYVNTYGTARVNL
                     QDSEIAEKLLSIFDMRPYAIEKRLKLRNPIYSETASYGHMGRKPETITKSFKLPDGKE
                     KRLKVELFTWEKLDKVKELRKEFGLK"
     gene            40585..43428
                     /gene="gcvP"
                     /locus_tag="PKM27_04955"
     CDS             40585..43428
                     /gene="gcvP"
                     /locus_tag="PKM27_04955"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNR06644.1"
                     /translation="MSHPHAFEQRHLGNGPVEVGEMLKTIGLETLDELIDQTIPPTIR
                     MTSAPDLPEAMDEYRYLRHIKGIAKKNKIFRSYIGQGYYGTFTPSPILRNIFQNPGWY
                     TQYTPYQAEIAQGRLEALFNYQTVVADLTALPIANASLLDEGTAAAEAMIMFYNLAIK
                     NEQGARHKFLVSDAVYTQTIDVLRTRAKALNIELVVKPDLADDLADPSVFGILLQYPG
                     KEGQLTDEAALCEAAHANQVHVAVATDLLALTLLKPPGEWGADAVVGNSQRFGVPMGF
                     GGPHAAFFACRDEFKRSIPGRIIGLSVDQDNAPAFRMALQTREQHIKREKATSNICTA
                     QALLAIMSGMYAVYYGPAGLKQLGEEVHRKTSQLAAALAEQGYVLKHKHFFDTLAIQA
                     SPEIIEAIRTRAIARGINLYYTRDSVQLSLDETVTAEDLQDLYAVFEVTGARAGWDTI
                     PDKLKRTSRYLTHPVFNTSHSETSLMRYIKQLENKDISLVHSMISLGSCTMKLNAVSE
                     LLPVSMEEFASMHPFVPADQAEGYHYIFDHLERWLCAITGMKACSLQPNSGAQGEYAG
                     LLTIAAYHEHQGQTNRKIALIPESAHGTNPASAVMAGMKVVVTKTDAMGNIDVEDLSR
                     LADLHADQLACLMITYPSTHGVFETRIQEICKIIHDRGGLVYMDGANMNAQVGLTSPG
                     IIAADVCHLNLHKTFAIPHGGGGPGVGPICVNEKLAPYLPGHSLVKTGGSRATHAVSS
                     APWGSASILLISYGYIAMLGKEGVVNATKHAILNANYIKARLENAYQVLYSGELGRAA
                     HEMILDLRPFKGIGIAAEDVAKRLMDYGFHAPTLSFPVPGTIMIEPTESESKAELDRF
                     CDAMIQIKKEMDEIAQGEADPQDNVLKNAPHTCSQVISDQWSRAYSRAKAAYPLDYLK
                     DHKIWPMVSRVNNAYGDRHLVCTCPPVDAYSEQ"
     assembly_gap    44142..44241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            45232..45822
                     /gene="ribA"
                     /locus_tag="PKM27_04960"
     CDS             45232..45822
                     /gene="ribA"
                     /locus_tag="PKM27_04960"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNR06645.1"
                     /translation="MFRRLAESSIPTRWGEFKVIAYGIKDSDPTPHIVLVKEPIPEDK
                     PVLTRIHSECVTGDIFGSQRCDCGPQLDKSLEMIGKSGGVLLYLRQEGRGIGLINKLK
                     AYQLQDQGIDTAAANIHLGFKVDERTYEDALLILRDLGIHEIDLLTNNPDKIKAFEQS
                     GIILHQRIGLEIEPVRSNRYYLETKKNIMGHLLDLK"
     gene            45830..46777
                     /locus_tag="PKM27_04965"
     CDS             45830..46777
                     /locus_tag="PKM27_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNR06646.1"
                     /translation="MPVSRSLWHTDDTHSEIRSQELDLQAGHQLLRSLYSLISLGTEK
                     TIALGGVPPRIFDRMEVPYMEGSFSFPVKYGYSLVAVAEDGRCYHLMHPHQDRVPVLP
                     ESLTLLPSGIPPARATLISNMETALTAYWDAEPVKKEKILVAGFGLLGALVALLLRLK
                     GFEEITVFEPDPLRSGMARSLGFPSGDPGFDPGPFDLAFHSSGNPTGLQSCLEVMGIE
                     GRIIELSWYGFQKSTLALGEYFHTRRLRILASQVSSIPRKMAGEWNFARRKQEVLALL
                     ADSCWDKLEIEEGPFENSPALFDKIRLSQTKGLTYMLKY"
     gene            46781..47164
                     /locus_tag="PKM27_04970"
     CDS             46781..47164
                     /locus_tag="PKM27_04970"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNR06647.1"
                     /translation="MYSVRIRDHIMIAHSLPHEGFGVASGLHGATYIVDATFFSPVLN
                     EMNVVVDIGIAAGVLKKILSHLHFKNLDTLDEFKGRITTTEYLAAHIHRSLANEIKPF
                     FNGRLRIELGESHVAWASFEGETGS"
     gene            47161..48120
                     /locus_tag="PKM27_04975"
     CDS             47161..48120
                     /locus_tag="PKM27_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06648.1"
                     /translation="MIYYACPDPLAFVSGGNLLNAQIFRAFREAGRAFARIGWQDIPY
                     IDFAPKDFLLVDSIWINKIQIRDFSKIKATKIFFTHLLPSMLDLGIKRIQEIERLSCF
                     DFLLANSAFTRNWLDALMSGKSRIKTVQPWIKKTGMQESAVRDKFILVAAWQPIKQID
                     LLFDTLTRVDLPKGFRIHCYGDRQVNPSYTFKCMQILEKHPGLAEHILLEGVVSAEHL
                     ASVYVAGKCLIDSSRFETYGMALAEGLVNGLQVLALGQGNVIHLIGQGRGKYCRDLEE
                     LVQTAVLIFEDKLSLEEKPVQDILTSWSAFVSQFSFLDQIGRI"
     gene            complement(48121..49869)
                     /locus_tag="PKM27_04980"
     CDS             complement(48121..49869)
                     /locus_tag="PKM27_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR06649.1"
                     /translation="MDHPGYSGLGKNNRRVNFLPALSILFCLQWLLYLPLYLAKQAEG
                     SFLPAIESFSNLVSRQNPDVFRWSLDYGFVLALLALPASMSRQKWMAAGTAFLYLMLF
                     LFQAYFHFSWKVYGEIPVWTYDWALIKRVLPVFMKSMRIPPFLAYALLALLLALFYVM
                     VYRGHRVLSGWFSQNPGLKAWLIPCFVFFLPAGLYYTNKPVDASGYYPAIHWIARDMN
                     LAFTSKRVKSLPDLHRRLDYEKYDSLPLVSKPNIYLLFIEAYGTVAGVVKPYNREYLL
                     KLDSMQKRLAGQGWHSASALSNSTILGGRSWLGFTSLMSGVRIDNHPAYEKLLREFPQ
                     YPHLIRALNHQGYQTYRLNTMANFGETFEKLDSITQIFFDHRHWTRYSDLPYRGYRYD
                     YFGGIPDQYALNYWDEYVLDKNQSPFFLFFITLNTHAPFYHPPPLLDDWKSLDQIEKS
                     PHKRIRAEDGSPLERYTQGVIYDLSVLEKYILEKAGSNSLFILIGDHPPAGMEYLLNG
                     ATDTYATPIHLISKDPRWVRVFTARGFEPGMAPVFKKGSLLKHEGFYSLFMQAWAEVD
                     SLPPSRIPAYLPYGIE"
     gene            complement(49883..50587)
                     /locus_tag="PKM27_04985"
     CDS             complement(49883..50587)
                     /locus_tag="PKM27_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNR06650.1"
                     /translation="MIPPQENEALTKLHFDLLLYSLWIIFFTFVSILKRDVFYLAITA
                     LPGFAYWVAKYFLNHRALHFPYGPANAVTLLRMTGLIAVTLIHPVIPDLLLGILFTLI
                     CLGDLLDGYLARTFKMTSTIGEYMDKETDALFVLCTTFILFQKGFTGVWIVSLGLIRY
                     LYFIVVFYFLKPGRKEIKDKYARIIAVVVFVSILLCYFLPYAWSFPVLVFAAAALFIS
                     FGRGALAELGLLNLNR"
     gene            complement(50630..53503)
                     /gene="uvrA"
                     /locus_tag="PKM27_04990"
     CDS             complement(50630..53503)
                     /gene="uvrA"
                     /locus_tag="PKM27_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNR06651.1"
                     /translation="MKIRSKEEEEAISPPLAEDTRHSIYIKGARSNNLRNIDVWIPKR
                     KLVLVTGVSGSGKSSLIMDTLYAEGQRRYVESLSSYARQFLMRMKKPEVDYIKGICPA
                     IAIEQKVSTSNARSTVGSMTEIYDYLRLLYARIGKTYSPVSGEVVKRHEVRDVVDYIK
                     NLKAGSKIYLLVPLSTKYDDRTLGQELALLLQKGYSRILRNHELFQIEEVQKSKGWPL
                     KDKLSKLGNAHIAILIDRFVTNPEDEDFVNQMADSVLTAFAEGEGNAIVYDSETRKSH
                     LFNNRFEADGLQFLEPTPQLFNYNNSYGACPRCEGYGRVMGIDADKVIPDKTKSVYEG
                     AIACWSGEKGSLWTQELIQKAHLFDFPIHKPIHKLTKEQYRTLWQGNDYFYGIDAYFR
                     ELEEKTYKIQNRVILARFRGRTTCPECEGGRLRKEAAYVKINGIALPDLIHLPVDELY
                     EHFRALRFSKQDEKIASRILREVLQRLDIMCRLGLKHLSLERLASTLSGGETQRINLT
                     RTLGSNLTSSLYILDEPSVGLHPVDSNRLVEVLKQLRDLGNTVVVVEHEEELIRQADY
                     IIDIGPAAGIHGGKVVYTGSYEQFIKPNQPGLTAAYLLGSQSIEVPSWRRKWKDKLVL
                     KGATLNNLQDLEVKFPLNTITAVTGVSGSGKTTLVKHLLFPAMERQLADGHFGIIPGL
                     QSLEGDLRQIRGVEMINQNPIGKSTRSNPVTYVKAYDPIRKLYANQHLSRVRGYGPGH
                     FSFNVEGGRCETCQGEGEIVVEMQFLADVKLLCEDCKGQRFKNEVLEVKFNGKSMYDI
                     LELSVEEALTFFKDQPDIHQKLKPLFDVGLGYVKLGQASSTLSGGEAQRVKLASYLTR
                     EAGQEAILFIFDEPTTGLHFHDIRKLLDAFNALVENGHSVILIEHNMDVIKSADWVID
                     LGPGGGKEGGRLIFAGTPEDLVKVKDSKTGHYLKEKINHRAGA"
     gene            53773..54354
                     /locus_tag="PKM27_04995"
     CDS             53773..54354
                     /locus_tag="PKM27_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR06652.1"
                     /translation="MQVMLLSDQDLIQQYLNGSEQAFEVLLNRHKTKIFTSIYLFTRD
                     HEVAEDIFQEVFIKIIDTLRKGKYNHEGKFLQWALRISYNMCVDQFRRSKRQTQVSPS
                     ETFDVLNVIQASDDNMEQTMIKSQGIHRIQSLVDQLPPEQREVVILRHYADMSFKEIS
                     QLTRVSINTALGRMRYALINLRRLMEEKVELVY"
     gene            54494..55216
                     /locus_tag="PKM27_05000"
     CDS             54494..55216
                     /locus_tag="PKM27_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR06653.1"
                     /translation="MGRIFEVRKSKMFARYDRMAKQFTRIGKEIIIAVKAGGPDPDSN
                     AQLRRCIANAKSVNMPKDRIEGAIKRALGKDTENYDEVLYEGYGPGGVAILVEAATDN
                     NTRTVANIRSYFNKMNGSLGTSGSVSFMFKKMGVFKLHPAGLNPEELELELIDFGLEE
                     LGEGTGENEEPILVLRVPFNEFGTMQKALEDRKLTPISSEMEWIPLNTTSLDEERAEE
                     VLKLVAKIEEDEDIQKVFHNLA"
BASE COUNT        13946 a        14233 c        14787 g        13519 t
ORIGIN      
        1 gtcagaggtt ggaggttcga ggtacgaggt acgaggttaa aggtacgtag ttagaggtcc
       61 gaggctcgaa gccatccact gcccaccatc cactatccac caaaccgtat atttgcctcc
      121 ttctattctt gccaatgaaa aaagtactga taactggcgc agccggattc ctggggtccc
      181 acctttgtga ccggtttatc agggagggct actcggtgat cgggatggac aacctggtta
      241 cggggagcct gggtaatatc gaacatctgt tcaaactgaa agaattcgag ttttaccacc
      301 acgacgtcag caaatttgtt tatgtgcctg gccccctgga ttatatcctt cattttgctt
      361 ccccggccag tccgatcgat tatctcagga tgcctattca gaccctgaag gtgggttccc
      421 tgggtacgca caatttgttg ggcctggcca ggcaaaaaaa atcaaggatc ctgatcgcgt
      481 ccacttccga ggtgtacggg gatcccctcg agcaccctca gcgggaagaa tactggggga
      541 atgtgaatcc catcggtccg cggggggttt atgacgaagc taaaaggttc caggaagcga
      601 tcaccatggc ttaccatcat tatcacggcc tggagacccg gatcgcgcgg atcttcaata
      661 tttacgggcc gaggatgcgg gtggaggacg ggcgcgcctt gccggctttc ttttcacagg
      721 ccatccgggg ggaagacctc acggtttttg gggacggctc ccagacccgt tctttctgtt
      781 atgttgatga cctggtggaa ggcatttacc accttctgct cagcgactac cacctgccgg
      841 tcaacctggg caatcccgat gaaatcaccc ttatggaggc tgccaccgag gtattgcagt
      901 tggtcggcaa cagctcgcaa atcgtattca aaccgcttcc ggtcgatgac cctaagacac
      961 gacaacctga tatttccagg gccaggtcta tcctgggctg ggaaccgaaa ataggcagga
     1021 gggagggttt taaacatacc ctggagtact ttaaaaaagt cctgaaataa tgaaatcaga
     1081 caaaacgata ttgatcacgg ggggagccgg tttcatcggg tctcacgcgg tcaggcattt
     1141 tattaataaa tatccctacc ggatcgtcaa cctggacgcc ctgacttatg cgggtaatct
     1201 caacaacctg aaggacatcc agggtcatcc caactaccat tttgaaaaag ccgatatccg
     1261 ggaccgggat gccctggacc ggatcttcaa tttttataag ccggattgga ttatgcattt
     1321 tgccgcggaa tcccatgtgg accgttcgat catggaccct cttgctttca ttgaaaccaa
     1381 tgtgttgggc acagccaatc tcttgctggc cgcccgcaaa cactggcagg aactccaggg
     1441 caaattgttt tatcatgttt ccaccgatga ggtgtttggc agcctgggcg ccgaagggta
     1501 tttttcggag gatacccctt acgatccgag gtccccttat tccgcatcca aagcttcatc
     1561 ggaccatttt gtgcgtgcct accaccacac ctaccatatt ccggtcatcg tttcaaattg
     1621 ttccaacaat tacgggccct accagtttcc ggaaaaactg atcccggtcg tcatccagaa
     1681 catcctgaat gagaaaccca tcccggtcta tggggatgga accaatatac gcgactggtt
     1741 atatgttcag gaccatattg acgcgataga ccttatcgcc caccggggca aaaccgggga
     1801 aacctacctg ataggagggc ataacgaact caggaacatt gatttggtgc gattattgtg
     1861 taatatcatg gaccacaagc ttgggcggac taagggacat tcggaaaacc tgatccgttt
     1921 tgtcaaagac cggccgggcc atgaccagcg atatgccatt gacgcttcaa aattggaaaa
     1981 aaacctgggc tggctgccca tgatcaaacc tgaaattgga ttggaaatga cggtggattg
     2041 gtatttaaag gaaaaagaat ggctggccgg cgtaacctcc ggggcttacc aggcatacta
     2101 tgaacaacaa tatcaaaatc gttaagggaa tgtcgcggtt atttagatat gcctttttat
     2161 ttttcatagc atggagcctt tccgtagatg ctgaggccca aatccccgcc caggctgccc
     2221 ctcttcaggc cgaactccag agaaggggca tctccgaaca ggaattgcaa gccaggctgc
     2281 tgcaaaaagg cgtcgatctc aacgatcttc aaaatctgac tgctgaacaa gcccttcagt
     2341 tccaggccat cattgaagaa accataaatg aactggaaag ggagaaaacg ggaagggctg
     2401 tcgatacttc caaaccggcc gtcaggtcag cgcccgatac cctcgcttcc ccaaagccgg
     2461 acagtactac gcccaaagcc ccggatcccc ccggaacctt gaaaatctat ggccaggaat
     2521 tattccggaa taaaagcata caggcctaca ccagttcgga aaatataaaa cccccggaaa
     2581 cttatgtatt gggcgtgggg gatatcgtaa atgtccacgt attcggacgc tcgaaagttg
     2641 attttaagaa tcttgaaatc aattcgcagg ggtatatcca gatggccaat tatccaagaa
     2701 tatacatcaa gggccttacc tatcagcagg ccagggaagt ggtccggaaa acgctgaatg
     2761 cctatttcac cttccagccc aatgaatttg agatgaccat tgactattcc cgggagatca
     2821 gcgtgaatat ctttggcgaa gccaaccaga tcgggacttt ccgcttgccc gcgaccaata
     2881 ccgccatcaa tgccctcgtc gccgccggag ggcccaatga tatcggaagc atccggaata
     2941 tccgtttgat ccgtggacgt accgtcaaaa aaatagacgt ttatgaattc atcgccaacc
     3001 cggcggttgc ccaggatctc tacctggaag aaaacgacat catccatatt ccgctcgcgg
     3061 agcgggtggt cgcggtggag ggggcggtca ggaggcctat gcgctatgaa ttgattgccg
     3121 gggaaaacct ggttaagttg atcgaatatg ccggcggtct caaagatgat gcttaccgca
     3181 agaatatcca ggtgcggcgg tttatcgatg acaagcctca gatcatcgat gtgcctctca
     3241 gaaccttatt ggacgaaaag tccgatttca ccctgatgag cggggacctg gttcagatcg
     3301 cttcgatcac ccggccgctg gaagaatttg ttcagattga tggacaggta cagaccccgg
     3361 gatcctatca gtttaccgat ggtatgcgtg tgtcggacct gcttgaaaag ggaaacctgc
     3421 tgtttggcgc aaaaaccgat ctggccttcc tgatgcgcct caatgaaaac ggcaattatt
     3481 cggtcaaccg cattcatatc cgggcgatcc aggaaaataa aaacgggcct gaaaatattt
     3541 tattgaaggc cagggaccgg gtcatggtct attccctttc ctattacctg gattcctctt
     3601 ttatatacgt atccggcgca gtccgtaatc cggtaagaat cccttttgac tttcaacgta
     3661 acctcagggt cagcgatctg ctcaccatga gcggggggct cctgcctgat gcactcgatt
     3721 ttggtcatat ccggcgcatc aataaagaaa attatgtgga gaaggattac atcaccttta
     3781 attcgaaaac ggccctggcc cgccccggca ctgcagaaga tccggtcctg gagccggatg
     3841 atgagatcct gctttacaac gaccggactt tcaacgatgc ctccaccatt tctattgacg
     3901 gtgccgtgcg aaatcccgga tcctttgcct atggccccgg gatcaccctg aaagatctgg
     3961 tagtccttgc agggggattt tccttgtccg ccgcaaccaa ccggatcgaa gtattccggg
     4021 ttgtcatgga cgacaatcaa ccgacccgga ccgtggtggc cactttaaac atgccccggg
     4081 acatcaccgc tcccggccat cggcccccgg atttcaaatt gcagcccttt gaccaggtcc
     4141 aggtcagggc ggtcccccag tttgaaatgc agcaaaatgt gagtatcgaa ggagaagtcc
     4201 agtttcccgg cacttactcc ctgataaccg agaatgaaac cattaccgaa ttgctgcgtc
     4261 gtgcgggggg gcctactcag gaggccttcc tcccgggggt cacgctcttt cggacacagg
     4321 ccaatgcggg ttatatcatt ttcagtctga ccgatgccat caaatcccca tcgggcccgg
     4381 acaacctggt tcttaaggat ggggaccgga tcgtcatacc caaagtcatc gactttgtga
     4441 ccatcgaagg ggctaccgac gcctcagagc tataccagga gaaattgctg gaatccaaca
     4501 accggattca tattgcctgg gtgcccggta aaaacgcgag gtattatgtc aatgaattcg
     4561 cggcaggcgt ggcccgggaa ggctcgaaga gcaggatcac cgtcgaacaa cccaatggcc
     4621 gcattgcccg gaccaaaaat tattggttct ttaaggtcta tccggaagtg gagaaagggg
     4681 ccgtggtcaa ggtgggtgaa aaactgagca aacaaaaaac cggagatgag gaacggaaga
     4741 agatagactg gatgaaggtc atggccgata ccctgggcca ggccaccgcg gtcctgtcgt
     4801 tcatcctcct ggtgcgggcc ctggataaac aatagtccgg ccggagaatt attcctcggc
     4861 tttccggtcg gaatccgcac ggcgcaggat gacatcgcgg tgacggataa aactgcacca
     4921 ttcacattcc ggcttgtcgc atcccagatc aaactcgtga ttctggattt tctgataggt
     4981 atccaggatc tggttcccaa cgatatccag atcctcctgg ctgaacaggt attccttgtt
     5041 caggaatcct tcctggtcca gtttattgac ctgaaggaag atcacctggg cactggtggc
     5101 attccatgtt ttgtttttat cccggtcaag gagtaattta taaaaacagg cctggcgcca
     5161 gtaatttcca cccatggaac ccctccaggg catcttgagc ttgtccttgg aactggtgga
     5221 atccccgcgt cccgacttaa aatcaatgat ccgggcactg ccttccttga ccaggacctg
     5281 gtccagtttt ccatggatgg gcacctgctc aaaaagggta cggtccaggt aatattccaa
     5341 ccgaaagtcc cggcagcctt cccacaaggc ctggtgttgc ttatagaata aaggaagttg
     5401 gtgggtaaag cggcgcaaaa ggccttcgta ttcgtcgggt gtgaaatacc attcgtgttt
     5461 tttcatccgg gtgaggaaaa aatccaccag atagtccgcc tgcaatcccg gggctttgag
     5521 cgcagcattg aaaaaatcaa agagcgcttc atggacggca attcccagcc cgatatgggc
     5581 attgggtgcc tggggcgttt tcaggatctc ctcaaaataa aaacgcctcg ggcattcgag
     5641 gtatttatcc aggtcggtgc agctcatcac gtaatccgtg cagaattggc tgatcaggtc
     5701 atggtccagc aattccgggg gagtggattt tttattttgg ataagctgga atttttcgat
     5761 cagcaggttt tcatccacct gttggacggg cggatccgat ttcagaaatt gcctgagttc
     5821 gctggcgaag accgattccc gcatgggttt ttggtcaatg cccgccagcg accaggatac
     5881 gaccagcgat tttttggccc tcgtcatgga cacgtaaaac aagcgtcttt catcatgttt
     5941 ctggctggct ttggactggt ccagttgttt cagcccctcc ggtattttat aaggttgttc
     6001 gctccctaag aaatagtcct cccagtcctg ggccagggca ttcatgatcc acaccgaatc
     6061 gaattccttg ccttttgagc cgtgggcggt gagcaggttg atgccctgct cggccgcata
     6121 ttggctttcc agcggcaggt agatcccgaa attttccatt ttatggatcc ggtcgagcag
     6181 ggctgacaga tccatggccg gattttcctg gacttcattg cggatcatgg ccaggaagga
     6241 gttgatggca cggagatgcc agtttcggtc cacggaattc agggcccagc tcaacatacc
     6301 cgatttccgc aggatgtcct ccgtgatcag ggaaagggtt tgcacgggag gcagcgattg
     6361 gatccattcc cgggtggagc ctatgaatgc ggcgagggca ggcctgtttt tgaatccggc
     6421 ttccggccag ctttccgggt ccagtatttt acctgcgagg tttttattgt ccctggtttt
     6481 tctttccagc caggagaggg tagagacatc gtggggatcg aggccccagt atgggaggta
     6541 gagcaattgg atcaggaaag gttccccgct gtttttttga agaaattcgg cctggaggta
     6601 ttcgaggagc aggatcaggt ggcggatcag gggttcttcg agtaaattcc ggctggacct
     6661 gaaattgatg ggaattccgg ccaattcaag gaggtaaacc aggtcctgga ccgttttatt
     6721 ccttcggaag atcacggcat gttcgcccag ggatcccgga ttggtctccc agatcttgcg
     6781 gatatggtca taaagcgcca gggtttcctg ctggggattc tggtagaccc tgagttccgg
     6841 gggacggtct tccgcggagc cccgggttga caacaatttt ttgttgattt tcatccggat
     6901 ctggggcggg atttccagtt ccagtttgtg gatcctttct tccaggggtt caatgatggc
     6961 atcagccgca tccagcaggc tttgggtgga tcggtagttt tcggtaatgc atatgatcct
     7021 gatgtcgggg tacttcctga gaatggtgac caggttttgc tggtccgcgc cctggaaacg
     7081 gtaaatggcc tggtcgtcgt cgcccaccac gaataaattg ggctgatccc aatattcaca
     7141 gaggctgagc aggacgttgt tctggatcac gttggtatcc tggaattcat ccaccaggaa
     7201 atactggtag ttttcctggt accgtgccag gaggtctgga tgttctttaa aagcctgctg
     7261 gacctggagc aggaggtcat taaaatccat ccattgttgc tccgccattt tttcagcata
     7321 tcggtcgaag gtcttcaagg catcgatggc ccgttcatac ctggctttat cctggaaata
     7381 ggcgggttta aattcgcctt tgttcccgga tttacctccc cttttgtatc ggtaatccgg
     7441 gtctgaatcc atgcctgcga tatgttcccg gatgccctgg atgagtttcg gaaggtctct
     7501 ttgttctttt ttgatgctct cgtattgttt tgcaagccgc tggatatccc aatgcctgtc
     7561 cccgatcagg cgctgaagca catgatcgtc cggcaactcc aacaggattt tttcaatcag
     7621 ctcaaaccgc tggagatcgg atgccagcct gaatttttta taatcaaaaa aatattcggg
     7681 attctcctgg atcactttat tgcagaaggc gtggtaagtg aacaggtggg cttcgtgggc
     7741 cgcaggcccg atgaattgaa acaaacggtc ccgcatggcc tgcacgccag cctccgaata
     7801 agtgaggcaa aggatctgcg gtatggaagc atcggtcttc tccaggatat tcccaaatcg
     7861 ggcagccagg acctgggttt tccccgtgcc cggcccggcc agcaccatca cggggccgtc
     7921 aatggattcg accgcctccc gttgtccttc attcaggatg gataaggcct tgtaaaactg
     7981 gtccttcagt tttttctttt catgcattga aaaaagatcg ggagacggcg tggtggattt
     8041 tttcttcatt cgcggtaatc aaatataagg gaggacagat cgggggcatg ccgacgctcc
     8101 cgggattgtt cgtaagagta aaacaatttt cccagccggc tcagataagg caggcccagg
     8161 ttttcataat cataaaggcc gtcgttgacc accccgtcgg tattggcatt gacggtggcc
     8221 gcaaggataa attcaatttt atttttaaaa tcaacaatat aggctacatc cgtgaggtag
     8281 ccgtaagccc agcccacctt gttgaaaatc cggaggtgat ccggtatgcg ttcctttgag
     8341 tcgccgtaca taaagaactt gcaaaatcca tcgtggtaag tgctgtcgta cctggggaag
     8401 cggctttcag atggaagttg ggacatgcat ttgaggaggt accgccgctg gtccggatcc
     8461 agatcaaacc tcaggccctc gggcatatat tcatggaaga ctatggcccg gatcatttgc
     8521 tccatatcgc ccagggagaa aaaatttttg gcgctgaaat caaaaggcgc atgtaccagg
     8581 ctgtccccgg catagtgggc ctgacccttc aacagggaac ccagatccgg caccctgact
     8641 tcagcgggat tgtattgttc cgcctggtcg tagatcctga ggtcttcttc aaaaaaatgg
     8701 accgggttgg tgtaccggtt tgcgtcgaga tcaaactccg gcgcgctcaa ccggtggatg
     8761 atatgggttt tgtggaaccc caggctgcgg agacgctcat ggatataagc ctgcccggta
     8821 aattcataga gccggttgaa ggcatcgttg tcgctgacca ggaaaatctt ttttataaaa
     8881 tggcccacac tggcctgacc gttccaggcg ctggcatctt cgctgaatgc cgactgcgga
     8941 ggcctggcgc tgtccgcacg catggcggta aaaagatcga gagcggggtg accggcctgg
     9001 agccgtttta ttttttccgc ggccagcagg gctaccggca attttaccac gctggcggga
     9061 tagaaataat gaagcggccg gtaattccag cgcaagcttt tgaaatgagg ccggttggcc
     9121 cggtcccggt cgatctgggt atagatgatc tgcgcttcat accggtcgaa ggagtccagc
     9181 acctttttga ccagcgggct tgaatacaat aaaccattca gcctggattt ttccccggca
     9241 taagatccgc ggaagcatcc cgtcgtcagc aagcctgcaa agaagaccag gaaggcagcc
     9301 cggagtccag tcatcccgca aaaataggac ttcttcatgg ggtggctttt atcacacact
     9361 aaaagaaaaa ttgccggaca atggtcaaag cggtaatcac cagcaggata taataactgg
     9421 ccacgtgcgc atgagcataa tgattgagat atttgacgcc cagccatgct ccgaaagttt
     9481 ggccgagggc gaggaaaaga gccgttttaa gatcaaacag gcccctggaa tgaaaaagga
     9541 agaccgcaaa taaagtaaag atgccggtga ccaggatttt ggctgcgttg gcatttttcc
     9601 atggcttcct ggccagcata atcgtggcag ccaggaaaaa aacacccacc ccgatctgaa
     9661 tgaacccgcc gtaaactccg atcaggaaat aaacgggaaa gaggtaccag gcctcacggc
     9721 tttcctcatg gaggtcggct ttcaaccatc tttccggttt taaaacgagc acgataaaaa
     9781 tgaaaaccag gaaccaggga tataactgcc tgaattggtc atcattgacc cgggtggcga
     9841 tccaaatacc cgtgagggct ccaagggtca ccaggaaaat ggcccggccg gtgcccgcca
     9901 gcaggagttt accgctccgg tggaggctcc aggtacccgc tatgctttgg gtgaggatgc
     9961 ccagacggtt ggtcccgttg gccatggaag gggagaggcc gatcagctcg gtacaaaggc
    10021 tcagggtaat caccgaacca ttgccggcaa gcgtattgat gaccccggcg agcatgcccc
    10081 cgatgaccag gatgaattca ctgaatccca ttattcaggc agggattggc acaattcaat
    10141 cagtaccccg tgggtgtttt tgggatacag gaaaacaatc agtttccggt cggcgccttt
    10201 tttggggttt tcatccacaa aacggaatcc tttctttttc aggtcccgta ccgcttcatg
    10261 gatatccggc acttcaaaag cgatatgatg gatcccctcg cccttttttt cgatgaattt
    10321 gcggataggg ccatcctggt ccagggactc aagcagttcg attttggtat cgcccgcctt
    10381 gaagaacagg gtggtgactt tttcctcggg caccgtttct tttttataag gctcttcatt
    10441 catcagggtg ctgtatactt ccctggccag ggcaaggttt tttaccgcaa tcccgatatg
    10501 gtccaatttc agcatgttcc gcttgtttaa taacccggct aaattataaa ttaatattac
    10561 aactttattt aatatattgg agctttattc acctgcaccg atgaaatcaa aaaaggagat
    10621 cgtggacaac tggctgccgc gttatacggg ggtgcctttg gaacggtttg gagaatacat
    10681 catcctggtc aactttaata attatgtgga gctgtttgcc caatggaatg gcgtcccggt
    10741 ggagggaggc gaccgcccca tggacagcgc caccgccggc aatatctcca tcatcaattt
    10801 tggaatgggc agtcccaatg ccgccacgat catggacctg ctcagttcca tcaaacccaa
    10861 agcctgtctt ttccttggga aatgcggcgg gctccggccg gaaaaaacgg tcgttggaga
    10921 tttgatcctg cctatagcag ccatccgggg ggagggcacc agcaacgatt attttccccc
    10981 ggaagtgccc gccttgcccg ccttcgccct tcaaaaagcc atatccacca ccattcggga
    11041 gtaccagagg gattattgga ccggcacggt gtacaccacc aaccgcaggg tctgggaata
    11101 cgatgagcat tttaaagatt acctaaaatc cctccggtgc atgggtatcg atatggagac
    11161 ggccaccata tttatcaccg catttaaaaa ccagatccct gccggggcat tgctgctggt
    11221 atcggatgtg ccgatggtgc ccgaaggagt gaagacggaa gaaggggata aaaaagtaac
    11281 cgctgattac ctgcaaaccc acctgaaaat cggaatcgat tcgctgaagc aactgattaa
    11341 caacgcgcag acggtcaggc atctgaggtt ttaaccggtc acaggctttt ggtcctgccg
    11401 ccgtccaccg ccaggttgat gccgttgata taagacgctg ccgggctggc caggaaggct
    11461 acggccgcgg ctatttcgcg cggttggcca aaccttttaa gggggatctc ttcgatggct
    11521 ttttgctcca gcgactccct ggtcgcgttt agttttttag cccggaaatc aaagatctcc
    11581 tgcaatcgct ccgtgagggt atagccgggc atcacgttgt ttaccgtgat ttgataaggc
    11641 cccaactcat tggcaagggt ttttgcccaa ttgctcacgg cagccctcag ggaattggac
    11701 aaaccaagat ctgccagggg ttctttcacg gaagtggagg tgatgttgat gatccttccg
    11761 tatccttcgg atttcatccc gggtacgacg agctggacca gttcgtgatt gatcaggaaa
    11821 agtttccgga aggattcttc gattttatct accggaaaat ccagcagttg accggtgggc
    11881 gggcccccgg tattattgac caggatgtgg aaatggcgcg ccgaggtcag cgcattgact
    11941 tttttgtgca ggtcggcgcg gtcttcgtaa tcggccatca ggtagtcgtg gtcctggccg
    12001 gggtggatcc tgggcagggc atggagggct tcctgcatga ggtccctgga ccgggccagc
    12061 aaggtgacat tggcgcccag ggatgcgagt tccacagcga tggctttgcc aatcccctta
    12121 ctgctgccgc ataccagggc attgcggttg atgagtgaaa gatccatggg tccgaatttt
    12181 aatcaaatat gaaaataatt tttgaagccc cctttcttct gctcattgtt tgtatttttc
    12241 agtttatatg gaaccggcaa gcttggaata cgccctggcc aaagacgaac aggatccgct
    12301 gggttctttc cgggaaaggt atgcgctgcc ggtgaaccgc gatggggatc cttcgattta
    12361 tttctgcggc cattcgctcg gtttacaagc ctttcagtcc aattattatg tgaaggaaga
    12421 gatggatcga tgggccagac tgggcgtcaa gggtcatttt gatggggaac cggcctgggt
    12481 ggattaccag gacaagcttc agcagatcct ctccggcctg accggggcaa atccgggtga
    12541 agtggtgatt atgaataccc ttacggtcaa catccacctg atgctcgtaa gtttttaccg
    12601 cccgacccgg gaccggtaca aagttctggt cgaatacagt ccattcccat ccgaccagta
    12661 tgccattaaa tcgcagatcc gttatcatgg ttttgccgtg gaagatgccc tgatcgaact
    12721 gaaaccgagg acgggcgaat acaaagtacg gatggaggat atcgaagaaa tcatcgacct
    12781 gcatggccct tccctggccc tggtgtattt tggttcagtc aactacctca acggacaggc
    12841 ctattctttg ccttccctgg tccaaaaggc ccatgcggct ggcgccttct tcggactgga
    12901 tctcgcacac ggggccggta acctcctttt aaaattgcac gaatgggagg tggatttcgc
    12961 ggtctggtgc acctataaat acctgaatgg gggacccaac agcctggcag gctgctttat
    13021 tcatcaaaaa catcacgcgg ggacgccgat accccgcttc gagggttggt atggccatga
    13081 tcttcaaagg cgttttataa tggaacccga ctttattcca atgccaggag ccgcagcctg
    13141 gcaactgagc ggcccaccca tcttgtccct cgcagggatg cgggccggtc tggaagattt
    13201 tgaagaagcg ggcatggaaa ggctgaggaa caaatcggtt caattgaccg cctatttagg
    13261 ttctttgctg gattccttgc aggatcccag gttggtcatc atcactcctg ccgatccgga
    13321 aatgcggggc tgccagttgt cactcaggct ggatccaccg gataaaagga tctttcatcg
    13381 cctgatggac tccggcgtag tggtggactg gcgtgaaccc ggcattatca gggtcgcacc
    13441 cgtacccatg tacaacagct tccgggatgt gtatgctttc tacgtcatcc tgaagcaaat
    13501 tttaaaggat ttatgaggaa caagattttt ttctgttccg attttcacct tggcctcgac
    13561 gccgccgaaa cctcttcggt ccgggaaaag atgatcctgg aatggatgga gaagaacgaa
    13621 ccggaaatgg aggccctttt tatcctgggc gatatttttg atttttggtt tgagtacaaa
    13681 caggtggtcc cccgcggggg tatccggatc atggcagccc ttgcccggtt gcgggaccgg
    13741 ggcataccgg tccaggtatt cacaggaaat cacgatcagt ggatgtttgg atatttccag
    13801 gaagaactgg acattccagt gcaccatgac ccggtgtcct tcaccctgca aaataaaaaa
    13861 gtgatgctgg gccacgggga cggactgggc cccggcgacc atggatataa atttatcaag
    13921 aaagttttcc gccatccggt tgcccggacc cttttcggct ggatcccgcc cgggatgggt
    13981 ataccgcttg cccgctattg gagcggggct tccaggagaa aaggggccag ggaacatgcc
    14041 ttcctgggac cggagaatga atggctcatc cagtattgtg aaaaaaccgt gcataaggaa
    14101 ccggggaata ccgactattt tatttttggt caccgtcatt tgcctgtgta ctggagattg
    14161 ggtgacggtt cacattatat caacctgggc gactggctga ggtatcactc ctatgccgtg
    14221 atggaagagg gaattatccg gctggacttt taccggcagg aagggtccaa accgatcagc
    14281 aacttttaat attttagtca tgaaaactcc agcggtatca ttcctgtttt tcctcgcttg
    14341 cacttcggtc attttggtcc aacccgcttg ccggcatgca acacctggtc ctgaccccct
    14401 ggctgccctc tattccaata cccaggtgat cggcgatatt gtgtataaaa cagccggtcc
    14461 acattccctt aaactggatg cttacattcc tgtgacccgt ttgggtgaac cgccctgggt
    14521 tgaatacagt ccggatctta aaccggtatt gtttttcctg cacggaggtg ggtggaccag
    14581 cggcaacaaa atatcgagat cccttttttt aatgcctttc ctttcaaaag gtttttgtgt
    14641 agtcacggca gactaccgtt tgttgtctca ggccagcctg ccggaaatca tttcagatac
    14701 ccgcgcagcg ctccggtgga tttacgacca tagcaggcaa tataaatttg atacggcacg
    14761 cattgtggta tccggggaat ccgcaggtgg ccatcttgcg ctcatgaatg gggtgctgaa
    14821 tgaaagcagc cggttccccc ccgattcaaa tgaagcggga catgagctga aggtagccgc
    14881 tgtggtcaac tggtttgggg tggcagatct ggtaaaagcg tcttcagatt gggacagtgt
    14941 ttatttaagc caggttgcag gcccggatcc ggcaaaaaca acaactgtgc tgcgatcctg
    15001 ttccccggtg aactatgtgg atgcacatac tgttcccata cttacaatcc atggcagcga
    15061 agaccgcgct gccaactatg accaggcttt gttgttgcac gatatccttg gaaaaaaagg
    15121 gattaagaat caattgctga ccataccggg taaaaaacat ggcaatttta attcccggga
    15181 aatggatcag gcgatgcagg aaatttggaa ctttctcagt tcaattcaaa tcctaaactg
    15241 atcatcaggt tccctctatt cacatatcgc attttatggc tgatggtctg gctataccag
    15301 ccggcctttg gtcaggatac ccttcctgat tcgttcagcc tggtaaccag ggttccattg
    15361 gaggccactg ccgtggtcac ggatttttta gggaacattt acgtgatcag tcgtcaaaat
    15421 gaactcgttc accttgatga aaggggcctg gtgctgggaa gatactccaa taattatctg
    15481 ggccggcccc aactcattca caccaacagt cccttgcagc ttgtactctt ttacccggac
    15541 tttcagaccc tgctgatcct ggacaaaacc ctcaatgaat taaaaaggat ccggctcgct
    15601 gacctcggcc tgccttatat gtctgccctg ggttacagtt cggaccggga gctttggtat
    15661 tttgatgaac cggtgaggcg cttcaggagg atcaacatcc aggggaagct gatcctggag
    15721 tccagcatga cttttgtccc ttttgaaggc gaagtggtga aaataaggat ccaaaacaat
    15781 gaggtaggag cctggaccaa tgccggccgg atttttattc tcgatctcaa tggccatcta
    15841 aaaagacaaa tcgacctgga aggggagtgg atatcatgga aagaggacga aatggtattt
    15901 ttttataatc agacgatccg gaaccattcc ccaaagggct tgtccggggc cacggatctc
    15961 tccggattgg atggtcaacc ctataccagc ctggggctgc tgccggccgg aatcgtggct
    16021 accaacggga aggaggttta tattttccgc tcagttccgc gaaaagaaag aaattaattt
    16081 atttatggct ttggcagcga ccgggcgcaa aaggccgaag tctgtttttt cggggttccc
    16141 gttgaaaatc cacatcattt ccaataagat tcccttggag cggcaggctt caatcagcgg
    16201 agtttttccg agccggtaag cctcacggat gagccgtttg atccggttcc ggcgggtggc
    16261 caggggtgct attttttttg gaacgcttat cgcaaaccgg acgccttgat tttttgcttc
    16321 ctcggtggga tggatcagaa agatgattct cagaggggga atactgaagg atttcccctg
    16381 gtcaaagagg acttgaatgg atttgcgtga tttgagtttt tccctgggtg gaaagcggtt
    16441 tgagcccgaa cggatttctt cgggggccat ggatcggctt attttattct ggcctcatcg
    16501 gcaaccgtca gcttgtgcct gcctttggct ctccggttgg ataacacttt acggccactt
    16561 ttactgctca tgcgtgcacg gaatccgtgt ttgttggaac gcctgcgacg ggaaggctga
    16621 aaagtctgtt tcatgacggc ttaaattaat ttgggctaca aaaataaaga ttctattccg
    16681 aaatggcaaa ttttgatttc cggttggctc agtctaggaa ttcatggtct gcaaaaattc
    16741 ctcgttgttc atggtatgct gcatgtgctt gagcatgaac tcaatcgctt catccggttt
    16801 catatcggcc aggtgattcc gtaaaatgaa catcctttgc agggaatcct tatccataag
    16861 aagttcttcg cggcgggtgc tggatttggt gacgtcgatg gagggataga ggcgtttgtt
    16921 ggacaagtta cggtcgagct gcaattccat attgccggtc cccttgaact cctcgaagat
    16981 gacgccgtcc attttcgatc cggtatcgat gagggcggtg gccaggatgg tcagggagcc
    17041 cccgttttca atggctcgtg cggcgccaaa aaatttcttc ggcttttgaa gggcattggc
    17101 ttccaccccg ccggagagca ctttgccgct cgatggggcc acggtgttgt atgcccgggc
    17161 aagacgggtg atggaatcca ggaggatcac cacattatgt ccgcattcca ccatgcgttt
    17221 ggccttttcg agtacgatgt tggccacgcg tacgtggttg tcggccggtt catcgaaggt
    17281 ggaggagatc acttccgcgt ttacgcttcg ctgcatatcc gtcacttctt ccggcctttc
    17341 atcgatcaga aggatgatga ggtaacagtc cgggtggttg taggaaatgg cgttggcgat
    17401 atctttcaac aggaaggttt tcccggtttt gggctgggca acgatcagtc cccgttggcc
    17461 cataccgatc ggggtaaaaa ggtcgatcag gcggttgccg taatcttttc ctgccgggtg
    17521 ggtggttagt ttgaattttt tggttggaaa caagggagtg aggtagtcaa agggtacccg
    17581 gtcccggatt tgttccgagg tcagcccgtt gatcagggat actttgagca gggcaaaata
    17641 cttttcccct tctttcggag gacggattgc gccgataacc gaatcgccgg ttttcagtcc
    17701 aaacagtttg atctgggagg gagacacata tatatcatcc ggagaactca ggtagttgta
    17761 atccgcggat ctcagaaaac cataaccgtc cggcatcatc tccaggacgc cctggccttc
    17821 aatcatggca tccaggtcca cattaaagat ctccgcaggg atggcggcct ccaactcagg
    17881 ttcggcgact ccattgggca tggctggagt atcgttgacg acgggagtag gacggggagc
    17941 aggagaggga gtgggagcgg gggaaggaat aggcgctgag gtaggagcag aagttttcat
    18001 agcagccgga gcatgtggtt tgccatgggc cggctgtgca tggtgcggcc gacgtgattc
    18061 ccgcggttcc ctttgttccc ggtgttctct ttgttctctc ggttcacgaa attccctggg
    18121 ttcgcgttgt tcacgtggtt cccgttgttc gcgttgttcg cgttgttcgc gtggctcccg
    18181 ctgttcccgc tgttcccgtt gttccatggg ttcgcggttt cctatattta ccggttcagc
    18241 gtcctcctgg atggctttgc gcatccgggg cctttgtggt ttggaatcgt ggtgcccgtt
    18301 ttgtttttga tgagcccctt cttttctcgg ggctgtgtcc tgggcctcgg actcggtaga
    18361 gggcccggtt tccatggatg aagcggcagc gcctcccatg gcctgccggt ccaggatctg
    18421 gaagaccagg tcctgtttgt tgagcttgcg gttgcccttt atacccaact ggtctgcaat
    18481 ttccctaagc tcgggcacca acatctcatt gagacgaagt atatcataaa tcataaaaag
    18541 tcgaatacat taaagttcaa aaaaatggcc taaaaatgaa cctgtaaaac gcaattaaaa
    18601 attattacct cagtataaaa tccaaatctt gtaatgggaa cacttttaga aaagtagaaa
    18661 ttcagaagcc ggttggcggc ttttgacccc gcaaatatat ggatatttta cagtaggcgt
    18721 atatttgcat gaattttttt tcaccacatc tcatgctgac cttcaactac tttaccaatt
    18781 taaaagacaa gaccattgcc ggcctaaaaa aaaggaactg ggatgaacaa aagttgcagt
    18841 gggtggatga aatagccctc ctggactctg aaagaaaaga actgcagact gccctggacc
    18901 agttgaaaat gcaggtcaac caggcatcca ggcaaatcgg caacctgatg aaagaaggcc
    18961 gccaccagga ggccgaaacc accaaaaacc aagtggcggg ccacaaatcg gaaatcacct
    19021 ccctggagga aaaattcagg acaaacaagg agcggatcca ggaactgctg ctgaagattc
    19081 cgaacatccc ccatgaatcc gtcccgccag gggcttcccc cgaggacaac gaagtattca
    19141 aagcctggaa agcgcccttg ccggaattgt ttccaggcgc cctaagccac tgggatctgg
    19201 ccgaaaaata ccagttgttt gacctgaaac tgggagttaa aatcaccggt gcgggtttcc
    19261 ctctataccg cggaaaagga gccaaactgc agcgggcgct gattcaattt ttcctggacc
    19321 aggcgaccgc ggcaggctat gaagaaatca tcccgccact gctggtcaac gccgataccg
    19381 ccatggccac agggcagttg ccggacaagg acggccagat gtattactgt gagcgggacc
    19441 aactctacct gatcccgacc gcggaagtgc ccgtcaccaa tgtttaccgg gacgaaatcc
    19501 tggaagaaag tgcccttccc attaaacttt gtggatacac tccctgtttc agaagggaag
    19561 cagggtctta cggagcggat gtcaagggac tcaacagagt tcatcagttc gacaaggtgg
    19621 aaatcgttcg cctggagcat ccggatcatt cttatcaggc cctcgataaa atgcttgaac
    19681 acgtgagcgg attattggat cggttggaat tgccctaccg gatattaaaa ctttgtggag
    19741 gagatatggg atttacctct gcgcttacct acgattttga aattttttcg gctgcccaga
    19801 aaaaatggct cgaagtcagt tcggtatcca attttgagac tttccagtcc aaccggatga
    19861 agctccgttt tcgcaacgcc gaagggaaaa cccaattgct gcacacgctc aatggcagcg
    19921 ccctggccct ggcacgcatc gtcgccggct tactggaaaa ccatcaaacc ccggatggga
    19981 tccggatccc tgcggccttg cagccgtata ccggattttc ccggatcgat tgaatctttt
    20041 tcttgcaaat ttgttattta aataaaaaaa atgaatatgt attcaagcgg ttattttctt
    20101 ttaagcatag gcctggcagc cgtcggtttt gtggtgcagc agatcctgct agccaagatg
    20161 cgcaaatacg gagcggcacc ggtctcgagg ggtatgagcg gcaaagaaat tgccgaaacc
    20221 atgctgaatt attacaatat ccgtggcgtt caggtcgtac ccggccaggg catgctcacg
    20281 gaccattata atccactgac caaaacggtc agcctgagcc ccgatgttta tgagggcagg
    20341 aatatcatgg ctgcggcggt ggccgcccac gaatgtggcc acgcggtgca gcatgccacg
    20401 gcgtacagca tgcttcagct gcgctccaaa ttggtgccgg tcgtccaggt ggccagtttt
    20461 ggacttcaat ggatgatgct tctttccttc atgctggcca acagttttcc ccagttgatg
    20521 ctggtgacca tcggcctttt tgcactcacc acgattttca gcatcattac cctgccggtt
    20581 gaattcgacg ccagcaagag ggccctagcc tggctcaatg cccaggcggt cctgaggtcc
    20641 ggcccggaat ccgaaggggc caaagatgcc ttgaaatggg cggccatgac ctatgtagtc
    20701 gctgctttat ccagcatcgt gatgctggtc tacctgatcc tcaggtatat gggactgagc
    20761 aggagcgatg attaatccgg taacttattt ccagttatga tgtcagcaga attagaaaaa
    20821 catatggcct cggtccagga ccaaatgagc aaggccgtgg aacatttaca acatgaattg
    20881 ttgaaagtgc ggaccggaaa ggccaaccct gccatggtcc atgaactcag ggtcaattat
    20941 tacggcaatc ccaccccgct caaccaggtg gcgaacgtgg ggctgagcga tagccgcacc
    21001 atcaccatac agccctggga aaaatccatg ctggccccca tcgagcaggc tgtttttgct
    21061 gcgaacctgg gcttgacccc gatgaacgac ggtgaaatga tccggatcaa tgtgcccccc
    21121 ctcacggagg aaagaaggaa ggacctggtc aaacaagcca aacatatggg agaggaggcc
    21181 agggtggctg tccggaacat caggcataaa tatcttgacg tcatcaaaaa agcggtcaag
    21241 gacggcaccc cggaagatga aggcaagaaa aaggaggccc agctcctgga actcgtcaac
    21301 cagcatgtgg aaaaggtgga caaactcatc aagtccaagg aagatgaaat tatgaaggtc
    21361 tgacggctat cccgctggtc ctttggattc cgtggacttt ataacccatt tgcctcatgc
    21421 accgactcga acagatagaa gaagcggtcc gttttgtccg gacacgcacg gagttgatcc
    21481 ccaaagtggg catcattctt ggcaccgggc tcagtggttt cagcgatgaa ttgaccggtc
    21541 gtcaggagat cccataccgg aggattcctc attttgtgga aagtacggtc aagggccacg
    21601 ccggcaagct ggtcttcggt ttattggaaa atgtaccggt cgtcatgatg gccgggcgat
    21661 tccattatta tgaggggtat tccctggacg aagtcacctt cccggtccgc ttgctccggt
    21721 acctgggagt ggaaagaatg atcatcacca atgtcagcgg ggctttgagg ccggaatacc
    21781 gggccgggga tatccttgcc gtccgcgatc acatcaattt gatcccatcc aatccgcttc
    21841 gcggccccaa tgatgaacgt attggccccc ggttcccgga catggtcaat acctacgatg
    21901 aaaaaatcct ggaaaaggcc ggcgaagccg caaggaccct gaaccttacc ttgcacaccg
    21961 gggtatacat ctcggtgccc ggtcccagcc tggagaccaa ggccgaacgg atctttttcc
    22021 agcatatcgg ggcggacgtg gtgggtatgt ccaccgtgcc cgaagtcatt gtctgtaaac
    22081 atatgaacct gccggtcaac gtattttcca ttatctcgaa tgaatttaat gtggaaacct
    22141 ccgccgaaga agtgatctcg gtcgcccagg cctatgaggg cagactcaga aacctgataa
    22201 aatcgatcct ggccggggtt aattcggata ctgcaggaaa ctaaaggggc atacctattg
    22261 ttttttcttt aatgctcgcc caggtgccca ttttatggtt tattgcaggg atgatcctga
    22321 ttatgctgga gattagcgtt cccggaggct ttatcatgct ttttttcggc ctgggagcct
    22381 tgacgctcag cgtattgact ttttttattg atatgggctg gatgttccag atcgctttgt
    22441 ttctggtctc ctcactgatg taccttttcc tgcttcgtga aaacgcccaa aggttcctca
    22501 acccgcagat aaaggaagat ccggaaaatg aattcatcgg aaaaaccgcg aaagcgctta
    22561 cgcccatcag cgatatggaa ggtaaggttg aattcaaagg cacgcagtgg actgccctga
    22621 gcaaagaacc tatcccgccc ggtactgagg tggaaatcgt cggacaggaa agcattaccc
    22681 tttttgtaaa accaataaat taaataaatc atgtttacag ccggttccat agcatatatc
    22741 atctttgccg ccttcgccct tctggtcctt tccagtgcgg tgatcatcgt tccgcagcgc
    22801 aaggcctata ttgttgaaag gcttggcaag tacctgggta ccctggcccc tggacttcat
    22861 ttcctgatcc cttttgtgga caaggtccgg tatcgtcatt ccctgaaaga acaggccctg
    22921 gatgtgaatc cgcaggtctg cattaccaaa gacaacgtgg cggtggaagt ggacggagtc
    22981 ctgtatttcc aggtcatgga tcccaccaaa gcttcctatg gcattgaaga ttatcgcttt
    23041 gcctctatcc agattgccca gacgaccatg cgttcgatcg ttggccgcct ggaactggat
    23101 aaaacctttg aggaaaggga gaccatcaac gcctccatag tagaagccgt ggacaaggcc
    23161 agtgaaccct ggggggtcaa gatcacccga tacgaggtta aaaacatcac gccgccccaa
    23221 agcatccggg atgccatgga aaaacaaatg agggccgaaa gggagaaaag ggccatgatc
    23281 gctgaatccg aaggtaccat gcaggccaaa atcaacgtgg ccgaagggga aaaacaggaa
    23341 gccatcaaaa aatctgaagg ggagaaatta aaaaggatca acgaagccga cggccgtgct
    23401 gccgaaatcg aagccctggc catagccacc gccaatggcc tccggaccat tgcttccgcc
    23461 atcgataccc ctaatggaaa agatgcggta tcgctccgga tcgccgaaca gtacctcaac
    23521 gaattcggaa aactcgccca gaaaaacaat accatgatcc tgccggcaaa cctcatggac
    23581 atcaccgggg tgctggctac cgccaaaaag gtactggatc atacccagcc tgcggacaac
    23641 ggaaaggcga aagtctgagg ggaaacccga tttttacgaa ttcattgggc ccctttccgg
    23701 tctagcttca atttggtttc aggtttagcc taattcgtcg ccgtccaatc tgaagtttgg
    23761 tttcattggt cttgagcagt atggtcggtt attttaccta cctactatca ggcacaaacg
    23821 tttcgtcccg ggtaaagttc aaccttaccg aagtgggtga tatcaaagca gaacttttaa
    23881 ggcaactgca accctgaaca ggccggcgga tcaatccccg ccctcgtaat tcatgtccac
    23941 gtcttctatc ggggattcgg cgatttcttc ggcatcttca tcttcaaaat cttttgaagc
    24001 cttgcttttg ggccgtttta ctccggattt tttgagttgt tttccttcca gggacctgcg
    24061 gatcacacgt tcatccgggg cgacctggtt gatgtccggg accatggagg cctgttcgat
    24121 gtgtttggcc tcaatgcttt tagtggcatg ggggaccagg agcagccttt ttttgtcgat
    24181 caggtttccg gtcacggtgc aaatgccgta agtcttgttt tcaatacgga ccagggcgtt
    24241 ttcgaggttt tggatgaatt gccgctggcg gatcgccata ttgttcagaa gctccacttc
    24301 agagtgaatg ctgctgtcat caaaccaatc gcttccctgg atgtcggcgt tgttttcatt
    24361 catttctacg atctgctcct gcagatagtt cagttcctga cgtgcgttta agagtttttc
    24421 atcgatcagg gctttgaatt cggccagttc cttgtcggaa taccggcttt taccatttgg
    24481 tttcggcaca ctgcccgttt cggggatttc tttgctcact gattttatat tttgagtttt
    24541 tgttaagcgg ggtggagcgg gctttgcagg cgcaggcccg ggggttttgt gaggttctgg
    24601 gtgtttgatg gcggttgcgg atttggttac cggcttggcc atcgtggttt ttttggtttc
    24661 cgcagccttg ggttgagcct tggccggagt cgctttggcc gtcgcggcct ttgcaggggt
    24721 ggatttactg gttatggttt ttgttttact gcctgcttta gttgcagtcg cttttaccac
    24781 actggcaggt gccggcttca aggctttgga cggtttggcc ttttcctttt ttttcgcaga
    24841 agctgcaccg gaagattttg ttcctttttt tgcacttgcc tttgttttgg atgttgtggc
    24901 cataaacaat tgaatttcag taaataaaga atataaattt tttacattga acctaaattc
    24961 agggcgcaaa tatacggttt atttgaaatt aaaacggcat attcggctac ccatatcttc
    25021 catccaacag actccatttc gcggatatga acgattgaaa atcaaacaac aaggccggct
    25081 gcagaatagt tcagcatctg ccgccggtat tcctattgca gaaagctata attttgtttt
    25141 atgcctgacc tgatccacct gcattgccat actcaatatt ccttgctgga tggcgcatgc
    25201 gggatacccg acctgataaa actcgcaaaa aacgatgggc agcaggggat tgcgattacc
    25261 gaccacggca acatgtttgg cgcgttcagc tttgtccatg aagccaacaa ggcagggatc
    25321 aaagccatcg tcggctgtga attttatctt gtagaagacc ggcgcaaaca ggtattttcg
    25381 aaaataaagg gggagaagga cgaaaggtac catcagttgt tgctcgccaa gaaccagaga
    25441 ggctacctca atatctcaaa gctttgttcc ctgggtttta tagaagggtc ttacggcaaa
    25501 tttccacgga tcgataaaga acttatcctg caatatcacg aaggcctcat tgccacctcc
    25561 tgctgcctgg gtgcggagat ccctcaggcc atccttgcgg gtaaactgga actggccgaa
    25621 gaactcgtca aatggtggaa gaatgttttc ggaaaggatt tttacatcga attgcagcgt
    25681 cacggactgg aaaacctgga taacagcggc ctgagccagg aggatgtcaa ccgggtactc
    25741 atccagttgg ccaaaaaata caacatcaaa accatagcca ccaatgacgt gcattacctt
    25801 acccgggaag actgggaacc ccatgacatc cttctttgtg taaataccaa ttcaaaaaaa
    25861 acggaaacgg aacggttccg ttttcccaat gaccagtttt actttaagtc ccgggaagaa
    25921 atgggcaggc tctttgccga tattccgcag gccctcgaac ataccctcga aattttttcc
    25981 aaggtggata agctggacct ggccaggaat atcctgcttc ccaatttccc cattcccgca
    26041 ggctatgcca gccaggatga atacctgaga catctggttt atgaaggggc caggcattgt
    26101 tatcccgagc tcagccagct tgtgttggac cggatcgaac atgagctgcg catcattttc
    26161 ggaatgaatt tttcagggta tttcctgatc gtccaggatt tcatcaaagc ggccagggaa
    26221 ctgggggtcc gcgtagggcc tggccggggc agtgccgccg gatctgtggt agcctattgc
    26281 ctgggcatca ccgggctgga tccgatcaaa tacagcctgc tgtttgaacg attcctcaat
    26341 ccggaaaggg tcaatatgcc cgatatggat attgattttg atgatgaagg ccggcagaaa
    26401 gtgattgact atgtggtgaa aaaatacggc agggaccagg tggcccagat cgtaaccttc
    26461 aacaccatgg cggcccggtc gtcgatccgg gatgtgggcc gcgtgctgga ttatccgctg
    26521 tcggatacag accggctggc caagctggtg ccggccaaac ccaaaattta ccttgccgat
    26581 atactcaata agccggtgga cgatcttaaa gcggccctta ccccggatga gatcaaccag
    26641 gtaaaccagc tccgcgatat ccggtccggg ggtgaccagg cgtccacggt cctggctctg
    26701 gcggaacgtc tggaaggttc ggtccgcaat accggtatcc acgccgccgg gatcatcatt
    26761 gcgccggaag ccattgaccg cattatgccc gtgttcactc ttaaagacac ggaactcctg
    26821 gtgacccagt acgaaggcac cttgatggag gatgccggca tgttgaagat ggatttcctg
    26881 ggactgcgca ccctgtccat catcaaggat gccctgaacc tcatcgaaga aaataccgga
    26941 acggcagtga acattgatca gatcccgctg gacgatccgg ccaccctgga gttgttccag
    27001 cgcggcgaca tgatcggcat tttccagttt gaatcggacg gaatgcaaaa atacctgcgg
    27061 gacctcaaac cggaatccat tgaagacatc atcgcgatga gcgccctgta ccgtccgggg
    27121 cctatggata atatccccag ttttatacgg cgcaagcgcg gacaggaaca ggtggaatac
    27181 ccgcatgagc gcctcgaaga attgttgcgg cccacctacg gcatcctggt ttaccaggag
    27241 caaatcatgc agaccgccca gattatgtcg ggttataccc tggccgccgc ggatttgctg
    27301 cgaagggcga tgggcaaaaa gaaacacgat gaaatgcagg tgcaccgcaa attgtttgtc
    27361 gaaggagcca tcgaacaggg gattgatgaa accaaggcgg aagagatttt tgaattgatg
    27421 gcccgtttcg cggactatgg tttcaaccgt tcccatgctg cggcttattc catcctggca
    27481 tttcagaccg gctggctcaa aactcattat cccaggcagt ttatggcggc cgtattgacc
    27541 cacaataaac aggacatttc caaactggaa tattacctgc aggaggccaa aagactgggc
    27601 attaaagtgc tggggccgga catcaatgag agcggtttaa actttaccgt cacccgggac
    27661 caggccataa ggtttggact ctccgccctg aaaggggtag gggaagggcc cgtggaggat
    27721 atcctccggg aacggcaggc ccatggcctc tttaccaaca tctttaacct cacttcaaga
    27781 ctgaattcac gtacggtcaa taaaaaaagc cttgaagccc tggtgctggg gggcgccctg
    27841 gatgccttgc accccgaggt ggaccgcagc cagtattttg cccccaccga caaatacgac
    27901 agtttccttg aatatgccct gcgcctgggc agccaatggc aggagatgaa atccaggaat
    27961 gaaatgtctc tattcagcct ggagagcgat acccccttac cggatccggt ctttcccaaa
    28021 atccgtgaac cctggagcct ggtcgagagg ctgaaccggg agaaagaagt gaccggaata
    28081 ttcatatccg cccatccgct ggatgaatac aaatacatcc tcgaccattt tacaacctgt
    28141 acggtggcaa acattcccca gtacaaggac atgcccatcc gggtcgccgg gattgtgacc
    28201 cgttccgatc accggatcag caagaaaggg accgggtacg ggcagtttga attgagggac
    28261 tttgagggca cctttgagtt tcctctcttc agtgaaaatt acctcgactt caagaaccgg
    28321 ctggagctgg gacaaatggt ctatatcaaa gggacttacc aggtatttaa tgacggcaat
    28381 tccagccgct tcaggattca gagcgtccag caacttgcca gcgccggccc atccatagct
    28441 gaaaacgtgc tcctccaagt accggtccat cagctggacg aaacgaaaat tgcaaaactg
    28501 aaatccatct gcgaaaacca gaaaggaaaa tccagattaa aaattgtgct cgtggaccgg
    28561 gccagccaga ccagccttca aatggtctcc gaggaataca ggatagaagt caatgccgat
    28621 ttgttgaatc aattcaaggg gctgggtttg gagtatgtgc tgaattagta gtcagtagtc
    28681 agtagtcagt agtcagtagt caatagtcag tagtcagtag tcagtagtca gtagtcagtt
    28741 cgcatctcgc atctcgcatc tcgtatctgg tatctcgcat ctcgcatctc gcatctcgta
    28801 tctggtatct cgcatctcgt atctggtatc tcgtatctgg tatctcgcat ctcgtacctg
    28861 ttctattctt cctccattcc gtgctattca tttaagacat cgttaacgtc ccggtcgggc
    28921 aaggccatat aaatttgtta atcttcatga gaaaggttgg ttggataggg ctgatgatct
    28981 tctgctgctt tgggatttcc gcccagagcg aatccccgcg caatctttcc cttgaggaat
    29041 ttaaggcact ttgcgccggg gaggccggta aatccgtcat cctcaatttc tgggcgacct
    29101 ggtgcggacc ttgtatggaa gaattgccct ggtttgatcg tttgaaaaag gaaatgcccg
    29161 gagtccggat catccttgtc aacctcgatt ttgagaaaga agtgaaatcc aaagtatggc
    29221 ctctgttgga gaaagggtat aaacaatgcg aagtgtaccg gatccaaggg ctccatcccg
    29281 acgactggat gcccctggtg gaaccggcct ggtcgggcgc cctgcctacc accctgatcc
    29341 tgaacaacgg caaaagaaag tttttcgaac agaaatttga aggatacgac tcactgaaaa
    29401 atgaaatcgt tcacttttaa aacaacactt caatcatgaa aaaatcattg tatttggtcc
    29461 tggctgcggc ttgcctcagc ctgtttttta cccttccctc tccccagggg taccaggttg
    29521 gggatgtggt cgccgacttc agcgctaaag acgttaccgg caaaatgatc agcaccaaag
    29581 attttcccaa agccaaaggg tatgtgatcg tcttcacctg caacacctgt ccttatgcca
    29641 aactttacga agaccgcatc atatccctgg ccaggaaact ggacgatgcc gcctggccat
    29701 tgatcgcgat caattccaat gaccggaaca tgtcgcccgg ggattccttt gagaaaatgc
    29761 aggagagggc caaatccaag gcttttcctt tcccttatgt gtacgacaag agccaatcca
    29821 tagtccaggc cttcggggcg agcaaaaccc cgcattttta tattctggat gcgtccaaga
    29881 aagtcaaata catcggggcc atggatgact acccggagga tgaagcaggc gtaaaaaccc
    29941 attacgtaca gcaagccatc caggcaatag aaaaaaacca ggatcccgat ccttccttca
    30001 cccgggctat cggatgcggc atcaaaaaga ggacgacctg atcctttgag gtcggttcaa
    30061 actttacaag ccggtgcagc aggccaccag ggctctcact ttggcggggt ctgagtccag
    30121 gggatcaccg gattgggagg tcatcgcggc aggatcatat tttgcggaac tgtgaatttc
    30181 ttttacctgg ctgtcccgga ccagttccat gacattgtca gggctgaccc cgcttcccgc
    30241 gaggatacga agccggtcac cggccagttg ctgtaatgct ttaagttcaa acctgttatt
    30301 gggagcatgt ccggtccggc cgccggtcag gatgcgatgc acaccaaggg cgatcagggt
    30361 ttcgagagcc tgtctgctgt ccggaacaga ttcaaaggcg cggtggaagg taaacggaag
    30421 accatctgcc gcggcgacca gtcttcgcat ggattccacg tccacctgat ggtccgcggt
    30481 gaggcaaccg gctacaatac cttgggctcc ggccctgact gcatgccgga tgtctttttc
    30541 cataatggat agttccgccc cggaataata aaaatcaccg gtgcgcggac gaatgagcac
    30601 aaaaacggga atacggacct cttcgagcac ctgctcgatc aaaccgaggg aaggggtcaa
    30661 tcctccgccc ccaaggtggg tgcataactc gatccggtga gccccgtttt cactggctgc
    30721 cagggcagcc cggtaggatt gggcgcagat ttcaacaagg accatgcgtg aggggtataa
    30781 acaataaagg gttcggtata aactatcgaa ccctttatgc atcctagagg tactggcctt
    30841 tgtaaggctt cggttttgtg gcctggatcc ctatgaacag ggaagccacc accatggcaa
    30901 tactgaatac gatcatggaa tggaatttat aattgtcaaa tcaaaaatag cttttaaccg
    30961 gtccctcata tgttaatttt ttatcaacta aatactaaca tatcttaaaa tcttattcac
    31021 gatcattcca atcccatgcc aacttttgtt taatttcctt aaaaacagga ctttaactaa
    31081 aaagagaatg gtgtgtggtg gttggtggtc ggcagggaaa gaggtataaa caaatcgaac
    31141 ggtaataata cggaagacga tttccctggc agaaaggttt gtgagccgct gggctaattt
    31201 tccacgctgg ggaccatcag ttggaactca gggatctcga tatcaaaaat ttcctggttg
    31261 tcgaggcgga tcatctggta gatcccgtgc attttaccga tatcgctggc aatgggacag
    31321 aaggattcat accggtgggc atccccgggt tggattaccg gctgttcgcc aatcacccct
    31381 tctccgcgca cctggcgacg ggtcccgttg ctttcccaga tccaccaatg ccgtctgagc
    31441 aactggactg cgaaggggtt ttcattggct atactgatgt ggtaggtaaa gacattctga
    31501 tccgaaaaag gaatggaata cttcggttga aaaaatacct ccacggccac tttgacgccc
    31561 agggtggttt tttgtaccgt gttggatgat ttattcactt gcctttgata aatttttcaa
    31621 ttaataattc aatttctttt ttcgcggttt cgaggtcgtc gttgatgacc agggcgtcga
    31681 actggttttc aaaatccatc tccattgagg cccgggccat gcggacagcg attttttccg
    31741 gggtttcggt attccgggct tgcagccgct gacgcaagac ccccaaagat ggaggtttta
    31801 taaaaatagc cagcgaattt tttgggaaca tccgtttaag gtctttggcc ccccacacat
    31861 cgatgtcgaa gacaataatt tttctcaggg cccaaagcct tttcagttcg cttttcaggg
    31921 ttccgtaaaa ctggccggga taaacctcct cccattccac aaaccggccc tgcctgaggt
    31981 attgcacaaa gctggaaacc gtgatgaaat aatattcgcg gccatcggcc tcataaggcc
    32041 tgcgtgcacg ggtcgtggcc gacacggata gcgcgaggcg gggaaaggtc tgcaacaggt
    32101 ggttgacgat cgtgcttttg ccggagccgg aaggggcggt aatgatgatc agttttccca
    32161 taagcgtcag accacgttag ccagttgctc cttgattttt tcgagctcgt ccttcatggc
    32221 gaccacgagg tgctggatat tgtgttcctg cgctttcgaa cccagggtat tgatctccct
    32281 gcccatttcc tggcttataa aattcagttc ccggccattg ctggcaggca ggttgagttt
    32341 ttcaatgaaa taatcgcaat gtttggccag cctgattttt tcttccgtaa aatcgatttt
    32401 ttcaaggtaa tacataagtt cctgctcgaa gcggttggcg tcataatcag gccggttggt
    32461 gaattcggtc atcatccggt tgaggcgcag cctgacctgg tcggtccggc ctttctcaaa
    32521 ggattccacc tggctgaggt attgtttgat attgactacg ttggtctgga tcaccccagc
    32581 gaggatcagt ccttcatgcg cgcggaaggc attgaattcc tcaatagccg ttttgatgat
    32641 attttctgcg accgcccatt cttcatccgt gagcggggat tccgaagatt ccaccacatt
    32701 gggcaaagaa agaatggacg ggatcagggg ttcacggccg agcccggcct gggagctcaa
    32761 ctggtttaat acttccgcgt aacgtacaaa gagggaggaa ttgatctgga cctcattgag
    32821 atctgcatct gcctgctcga tcagaaaatc gattttgccc cggtcaatac tcccgttgag
    32881 caatttccgg atctcgaatt ccttatcccg gaacaggatt ggcagccgga agcggtactc
    32941 ggcaaatttg ctgttgacgg atttgatctc cacccggatc ttgtgattcc ggatctctcc
    33001 cccggaacga ccgtatccgg tcattgatat taacatggct gcaaagatag atctccgggt
    33061 gcatccgaca aaacaatgaa aatcaaccgg gaataaatgg catgccgacc cgtttcgatc
    33121 aggaggaatg gttggatacc cgctggacag agaataaaag cgctgcccga aatgcaagac
    33181 ttttgaagac catctaggaa tagtgttgtc aaaatgttaa aaactatatg gaagccccat
    33241 ttcatattaa ggaaaagtga caaatctgcc tcagggactt aactttagcc attcatttca
    33301 aatcgatttc ttatgccaat gtacaatctg aacatcaacg gaaagcagca taaagctgag
    33361 gtttccgatg ataccccatt gctctgggtg ctccgggacc acctgggatt gctgggcacc
    33421 aaatacgcct gcggcatcgc cacctgcgga gcgtgtacgg ttcatttaaa cggggaagcc
    33481 ataagatctt gtgtcctccc ggtatcctcc gtcggcaatg cccaggtgac tacgatcgaa
    33541 gggctttccg ccaacggcga acacccggtg cagaaagcct gggacgaggt cgatgtgccg
    33601 caatgcggtt attgccaggc agggcagatc atgaccgccg ccgcttttct aaaaaagaac
    33661 cccaatcctt cggataagga aatcgtggaa gccatgaatt caaacatttg ccgttgtggt
    33721 acctatcacc ggatcaagga tgcggtgaag attgcagcca aaaacatgaa ataagtttat
    33781 tcctcactcc ctgaaaatta aaatccatgt caacgtcaaa aatagcctca cccaaccgga
    33841 gacaatttct gaaaaccacc acattggccg gtggcggact cttcctgagc ttcgatcttt
    33901 tcagtaaaaa cagcagctcc cggatgaaag tgcagtccgg ggaggcggat ctcgttcatt
    33961 tcaactccta cctttccata gatccggatg gtacggtgac cattttttcg ccgaacccag
    34021 aagtgggcca gggcataaaa acctcttttc ccatgatcat agccgaagag ctggatctgg
    34081 actggaaaaa aataaaagtc gttcaggccc cgctggatac caaacgtttt gaaagacagg
    34141 tcgccggggg cagccggagt acgccccatt cctggaaaag gctgagagaa gccggggctt
    34201 cggcccgcaa tcttttatta caggctgcaa gtgcccaatg gaaggtgcct atggaccagt
    34261 tgtccacttc catgggaatg attcaccata aggccacggg caaaatggag cattacggag
    34321 ccttcgccgg agcagcggcc aaactggacc tccccaagga aatccccctg aaggacccgt
    34381 cccagttcca taccattggc acctgggtga agggcgtcga taatatggaa gtgtttaccg
    34441 ggaagaccca atacggactg gatatttata aaccgggcat gaagcaggcc atgattgtac
    34501 gggcgccggc tttcggcctg aagctgaaat ccgtaaacgg cgaggccgcc aaatccatgc
    34561 cgggaataga agaggtggtt tccttcaata accgggtggc catcattggc aaatcgacct
    34621 gggagatcat gaaggcccgg aaagcgctga atatcgaatg gcaaaaggat acggacctcg
    34681 aaagttccga aatgcataat aagatcctgt cggatatgat cggcagcggc aagatggaaa
    34741 cccgccgggc cgaaggcaat atcgaacagg ccttcaagga tgcagtaaaa acagtggaat
    34801 cggagtttca gtgcccgttt attccccata actccatgga acccatgaat ttttatgcgg
    34861 atgtccggga ggactccgcc gaattggttg ggcccacaca gaatcccgaa agcgcgcgca
    34921 atgccgttgc acgcctgctc aatctgtccc aggacaaggt gaatgtggtg tttacccgca
    34981 tgggaggggg cttcggcagg aggctaaaca gcgactttgc ccttgaagcg gccgaattgt
    35041 ccaaactgat caagtccccg gtgaaagtaa cctggtcacg cgaagacgat atgaccgggg
    35101 gtatttaccg gccggcctgt aaatataaat tcaaagcagg cctggatgcc aatggaaaca
    35161 tgatcgcttt ccaattgagg gcagccggca tcaacagcgg caatccttcc cgggagaata
    35221 atttcccggt cggggccatc gaccatgttt tggtcgaatc ctcagattat aaatcgccca
    35281 tcacgacagg accctggagg gctccgatca ccaatttcct ggccggtgcg gaacaaatct
    35341 ttttcgatga agtggccgaa gccgcgggca aagaccccgt ggcgctccgc ctggaatggg
    35401 tggccaaagc caaagccaag ccggtcggca aggtattgta tgaaccggac cgcttcaagg
    35461 ccgtcatcga cctggttgcc gaaaaatcgc aatggtttaa gaaaaagaaa ggcgtgtaca
    35521 agggattcag cgtctatttt gcccactcca cctatgtggc ccaggtcgct gaagtggtca
    35581 aggtcaaagg caagcccgtg gtgaaaaagg tttattgcgc cgtggattgc ggcatcctgg
    35641 tcaatcaaag cggggccctc aaccagattg taggcagcgt ggtggacggc atgggagccg
    35701 ccatgtatgg aaggctgacc tttaaggagg gcagtcccaa tgaaaccaat tttgacaaat
    35761 accggatgat ccgcatgggt gaaataccgg atgtggaagt gcattttgta aaaaatacca
    35821 tagatcctac cggcctgggc gagcccgccc tgccgccggt gagcggtgcc attggcaatg
    35881 ccttgtaccg ggctacaggg atccggtacc gggaccagcc cttcatcgac tatatgaatg
    35941 aaggcaaagc gggcgccggc cagaacatgt agggccggga ttcaagccct ctctgccctc
    36001 tccggtagat tccgtgtgat gtaagccatc aaatgtttgt agtattattc ctgcaatagt
    36061 agggaaggaa tatccccatt tccctgcccg tgcaatgatt tcgaccggcg cctgcctgag
    36121 ggtatcgggt ggggatttta agaccgttta ccattccaaa attgcctgcg atccagtttg
    36181 ggaaaccctg cggaaaattt tacaaaaaag agtatctttg catcccgaaa aattgggaaa
    36241 agttctttca aatggaacgt tttaaggagt attcaatacc ggtttccggg cttaaaccgg
    36301 gtttccacga atatcgtttc gaaatggatc ccgggttttt cagccatttt gaaaaatcac
    36361 cccttgcgga tggccggttc aaagcagaag tgcttatgga tcgcaggcag gacttggccg
    36421 tactggattt caggattgaa ggcagctatg cctgtgcctg cgaccgttgc ctggcgccca
    36481 ttgaattgcc ggtgcacacc gaataccggc tgattctcaa atttgaagag ggcaaggatg
    36541 aagacgatgt gatttatctg gatgcccagg cttcggaatg gaatgcggcc cagctgattt
    36601 atgagttgat catcatggcc atgcccctga ccaatgtata tgattgcaag ggcattccct
    36661 gcaaccagga cgtgctccga caactggaaa agacagaaaa cgcgcccgga ggggatgcct
    36721 ccgtatggga agacctgaaa aaaataaaac tgaattaact gatttctttt aaatattaag
    36781 tcatgccaaa tccaaaaacc cggatatccc ggtctagaaa aaacaaacgc cgcacccacc
    36841 acaaggctga acttccgaac gtatcggtat gcaaaaccac gggggaagcc ttcctcaggc
    36901 atcgtgctta ctggcacgag ggagaccttt attacaaggg ccgcgtgttg gtgaaagggg
    36961 ccacggaata aagccggatg aaacaaatcg taaaaaccgg ggaggccccg cttccgatcg
    37021 ggccttacaa ccaggcggtg aaagccaacg gcgtcctcta tatttccggc cagatcgcca
    37081 tcaatccggc ggacggcagc ctggtcaacg acagcctcga agcggaaacc caccaggtcc
    37141 tgaagaacct gggcgccata ctccgcgcgg aagggttgga ttatgaacag gtggtcaaat
    37201 gcacggtttt tatcaaagac atgcaccagt tttcccggat caacgccgtc tatggcgaat
    37261 attttcccga ggcaaccgcc ccggcccgcg aaacggtgga ggtatccaac ctgccgagat
    37321 tcatgcaagt ggaaatttcc gctatagcgg tctactaatt cttttggatg aaacctgaaa
    37381 actccgaaaa agaaagggtc ctttccggca tgcagcccac gggcaacctt cattttggaa
    37441 ggtattttgg tgcggtaaaa aattgggtag accttcaggc ggcttacgat tgttattact
    37501 gcatcgtgga ttaccatgcc atgactatgc cctataaacc ggccaacctg agggcatcca
    37561 cctggaacgg gatctttggc ctcatcgcct gcggcattga accccggaac ctgtttattc
    37621 agtcctgggt ccccgaacac gccgaattga cctggatcct gagttgtttt gccagctatg
    37681 gagaggtgca gcgcatgacc cagtttaagg acaaaagcca gcaggtcgcc gaaaaagaca
    37741 aggaggcttt catctccgtg ggtcttttta cctacccggt cctgcaggcc gcagatatcc
    37801 ttatttacaa acccaaatac gtgccggtgg gtaaagacca ggaacaacac ctggaactga
    37861 cccgaaatat tgcgatccgg ttcaatcaac aagtggggaa agaaatattg cccgtgcccg
    37921 aggccttgtt taccgagatt ccaaaggtca tgtccaccgc tgaccccagc cggaaaatga
    37981 gcgccagcct gggggacaaa cacaacatca atgtatttgc cgatccggac cagatacgca
    38041 agcagatacg caccgctgtg acggataccg gaacggaaac ttcatccgcc atgagcccgg
    38101 gagtggcaaa cctgttttca cttttgaaag ctagcggtca aacggagact tatgccgaac
    38161 tgaaatcccg gtatgaggac caaagcctta aatacgcgga actcaaagaa gtggtagccg
    38221 aagccctggt ccgggtcagc gctcccatgc gggaaaaaat ggaccatatc caggccaaca
    38281 aaaaggaata caaggaaatg atccgggcct cttcggaatc catccgcaaa agggcgaaac
    38341 acaacctcga ccagatcaaa gacgcctgcg ggctgatgat cacttcctga gctctttcat
    38401 tcatttcaat aatttctctt accttttgcc ttcatccaaa cgatgcggca tgaaaatcat
    38461 ctgtgtagga agaaactata tcaaccacgc caaagaactc aataatccgg tcccggctga
    38521 accggtcctg tttatgaaac cttccacggc cctgctgatc aaccgccgtc ctttttattt
    38581 cccggatttc agtaaaaata tccaccatga actggaactg gtcatccgga tcagtcagaa
    38641 cggcaaagcc attcagcccg cttttgcgca caaatattac gattccgtcg gcctgggcat
    38701 tgacttcaca gccagggacc tccaggaaaa atgcaaggaa aaaggacttc cctgggaaat
    38761 cgccaaggcc tttgattatt ccgccgtcct gggagacttt atcccgcttt cagaattgaa
    38821 aaacagggag gccattgaat ttgagctccg taaaaatgag gcgccggtac aaacgggcaa
    38881 ttcgaaagat atgattttca gttttgacca gatcatctgc catgcctccc gctattttaa
    38941 actgctcaag ggcgatctga tctacaccgg caccccggcc ggggtagggc cggtggccgc
    39001 gggagacacg ctcaccggct acctggaagg ccagcctatg ttgtatgtca gtataaaata
    39061 agccggtatt tacaggctgt tccattcggg ataaatacat acttttgcag ccgttgaatc
    39121 caacgattcc tcattaaccg tttaaaccta atctatgcct tatctattta cttctgaatc
    39181 tgtttccgaa ggacaccccg ataaagtggc ggatcagatt tcagatgcgc tgattgatca
    39241 ttttctcgcc tttgacccgg aatccaaagt agcctgtgaa accctggtga ccacgggcca
    39301 ggtcgttctg gcaggtgaag tcaagtccaa aacctacctg gacgtgcagg acatcgcccg
    39361 tgaagtgatc cgcaaaatcg ggtataccaa aagcgaatac atgtttgaag cggattcctg
    39421 cggcatttta tcggccatac acgaacaatc ggccgatatc aaccggggcg tggacaagaa
    39481 aaacagggaa gaccagggcg ccggcgacca gggcatgatg tttggctatg cctgccggga
    39541 aaccgaggac tatatgccct tgccgcttta cctggcgcat caggtcctgc tcgaactcgc
    39601 ggccctgcgc agggaaaaca aacaaatcaa atacctccgg ccagatgcca aaagccaggt
    39661 caccattgaa tacaatgatc agaaccaacc ggtgaggatc gatactattg tgatttcaac
    39721 ccagcacgat cccttcgccg atgaccaggt catggcggaa aaaatctaca aggatatggt
    39781 gggtatcctg atccctcgcc ttaaaaaaag actggatctc aaaaaccaga aattgtttga
    39841 tcagaagatc aaataccata ttaatccgac gggcaaattt gtgatcggag gtccccatgg
    39901 cgataccggc ctcacgggcc gtaagatcat cgtggatacc tacgggggga aaggcgccca
    39961 cggaggagga gctttctcgg ggaaggaccc ttcaaaagtg gaccgctccg ccgcctatgc
    40021 cacccggcat attgccaaaa acctggttgc cgccggagta tgcgatgaag tgctcgtaca
    40081 ggtctcctat gccattggcg tagcccagcc catgggcctt tatgtcaata cctacggaac
    40141 ggcgagggtg aacctgcagg acagtgaaat tgctgaaaaa ctgctgtcca tttttgacat
    40201 gcgcccctat gccattgaaa aaaggctcaa actccggaat ccgatttaca gtgaaaccgc
    40261 ttcctatggc catatgggcc ggaaaccgga aaccataacc aaatcattca aattgcctga
    40321 cggcaaggag aaaagattaa aagttgaact tttcacctgg gaaaagctgg acaaggtgaa
    40381 agaactccgg aaagaattcg gcttgaaata acggcaggac ccgcctccct ttttttggaa
    40441 tactggctgc atcggctgat gggataagtt tgtagtccat tcgtcaccat ctaatatatt
    40501 ttgatatttc ttatataata ttgttccatt cgaaagaatt aagggagcca agtttgtact
    40561 tttgcgttca aatttgctaa ccatatgtcc catcctcatg cttttgagca aaggcactta
    40621 ggaaacggtc cggtggaggt cggtgagatg ttaaaaacca ttggcctcga aaccctggac
    40681 gaattgatag atcagaccat acccccaacg atccggatga cttcggcccc cgacctgccc
    40741 gaagccatgg acgaataccg ctatctccgg cacatcaagg gcattgcgaa aaagaacaag
    40801 atttttcgat cctatatcgg ccagggctat tacggcacct tcactccttc acccatactc
    40861 aggaatattt tccaaaaccc gggctggtac acccaataca cgccttacca ggccgagatc
    40921 gcccagggcc ggctggaggc gctttttaat tatcaaacgg tcgtcgcaga cctcacggcg
    40981 cttcccattg ccaacgcctc cttgctggac gaaggcaccg cggccgccga ggcgatgatc
    41041 atgttttata acctggccat taagaatgaa cagggcgccc ggcacaaatt cctggtcagc
    41101 gatgccgttt atacgcaaac cattgatgtc ctgcgaaccc gtgcgaaagc cctgaatatt
    41161 gaactggtgg tcaaaccgga cctggctgac gacctggccg atccctccgt gttcggcatc
    41221 cttcttcaat atccgggcaa ggaaggtcaa ttgactgatg aggcagcgct ttgtgaagcg
    41281 gcacatgcaa accaggtcca cgtggcggtt gccacagacc tcctggccct gaccctgctt
    41341 aaaccgccgg gagaatgggg agccgatgcg gtggtgggaa acagccagcg gtttggagtc
    41401 ccgatgggtt ttggaggtcc gcatgccgca ttttttgcct gccgcgatga attcaaaaga
    41461 agcataccgg gaaggatcat cggtctgagt gtggaccagg acaatgcccc ggccttccgc
    41521 atggccctgc aaacccggga acagcacatc aaaagagaaa aggcgaccag caatatctgt
    41581 acggcccagg ccttgctcgc catcatgagc ggaatgtacg cagtgtatta cgggccagcg
    41641 ggtttaaaac aactgggtga agaggtgcac cgcaaaactt ctcaacttgc cgccgccctt
    41701 gctgaacaag ggtacgtcct taagcataaa cattttttcg atacccttgc catccaggcc
    41761 agcccggaaa tcattgaagc catccgaacg agagcgattg ccagagggat taatctgtat
    41821 tatacccggg attccgtcca gctcagcctg gacgaaaccg ttaccgcgga agacctacag
    41881 gatttgtatg cggtgtttga ggtcaccggc gcccgggccg gctgggatac catcccggac
    41941 aaactgaaac ggacctccag gtacctgacc catcccgtgt tcaacaccag ccattcggaa
    42001 accagcctga tgcgttatat caaacaattg gagaacaaag acatttcttt ggtgcattcc
    42061 atgatctccc tgggttcctg tacgatgaaa ctcaatgccg tatccgaatt gttgcccgtc
    42121 tccatggagg aatttgcctc gatgcatccc ttcgtcccgg cggatcaggc ggaagggtac
    42181 cattatattt ttgatcacct ggagcgctgg ttgtgtgcca ttacgggcat gaaagcctgt
    42241 tccctgcagc caaacagcgg ggcccagggt gaatacgccg gtttgctgac cattgccgcc
    42301 taccacgagc accaggggca gacgaatcgg aaaattgccc tcatccccga atcagcacac
    42361 ggcaccaacc ccgccagtgc cgtgatggcg ggtatgaaag tggtggtcac caaaaccgat
    42421 gcaatgggca atatcgatgt ggaggatctc agccggttag ctgatctgca cgcggaccaa
    42481 ctggcctgcc tgatgatcac ttatccctcc acacacggcg tgtttgaaac caggatccag
    42541 gaaatctgca agattatcca cgaccgggga ggcctggtct acatggatgg ggccaatatg
    42601 aatgcccagg tcggtttgac cagcccgggc attattgcgg cggacgtatg tcatctcaat
    42661 ctccacaaaa cctttgccat accccacggc gggggcggac cgggcgtcgg gccgatttgt
    42721 gtcaacgaaa agctggctcc ctatctccct ggccacagtc tggtgaagac cggaggttca
    42781 agggccaccc atgccgtgag cagcgccccc tggggcagcg caagcatcct gctgatctcc
    42841 tatggatata ttgccatgct gggcaaggaa ggggtggtca atgccacaaa gcacgccatc
    42901 ctcaatgcta attatatcaa agccaggctt gaaaatgcct atcaggtcct ctacagcggt
    42961 gaattgggac gcgccgccca tgaaatgatt ctggatctga ggcccttcaa gggcataggc
    43021 attgcggcgg aggatgtcgc caaacggctg atggattatg gatttcatgc cccgaccctg
    43081 tcctttccgg taccgggcac gatcatgatc gaacccacgg agtcggaaag caaggcagaa
    43141 ctcgaccggt tttgcgatgc gatgattcag attaaaaagg aaatggatga aattgcccag
    43201 ggagaggccg atccccagga taacgttttg aaaaacgcgc cccatacctg cagccaggtg
    43261 atctccgatc aatggtccag ggcctattcc cgggccaagg cggcctatcc gctggattac
    43321 ctgaaagacc ataaaatatg gcccatggtc agccgggtaa acaatgctta cggcgaccgg
    43381 catctcgtct gtacctgccc gccggtggat gcttattccg agcagtgagg aggtcgggtt
    43441 gatctttaga ggtcagaggt tagaggtaag aggtaaggac ttcgcattaa atatctccaa
    43501 agcccctgac cccccatgta ctaatctcaa atcctaactt tggggcaggc cccgactacc
    43561 aattcttaaa tactgcctgg gggctggacc gcgggggaac caagggacct ggcagtgtac
    43621 tcaaccaaaa caatcttcct agcccttacc ggaataagca cagagctcgg tccattaaat
    43681 ttatttcgta tcgccaaggg tcgggttccc cttgaaggcc tgccccatat gtaagatttg
    43741 agaattagca ggtgggggac aggggtcgga gaataattac aaaaaaggaa attcatataa
    43801 atctcttaaa cggccaattg tcttaaacgc ccctgtcccc tgcagggaaa ccaaggagtc
    43861 tggcagtgta ctcaaccaaa acatcttcct tgcctttccg gattaatcac agagatcggt
    43921 ccattaaatt tatttcggaa tgccaagggt taggttcccc ttgaggggac aggggtcgga
    43981 gtacaaccca ttaatacata attttggaaa gaattttcct attgcccctg tccccgcagg
    44041 ggaaccaagg ttcctggcgt tatatcttaa taaaacaaac tcgcctgccc ttaccggaat
    44101 aagcacagag ctcggtccat taaatttatt tcgtatcgcc annnnnnnnn nnnnnnnnnn
    44161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44221 nnnnnnnnnn nnnnnnnnnn nttatttcgg aatgccaagg gttaggttcc ccttgagggg
    44281 acaggggtcg gagtaccacg cattaatatt ttcaacgccc ctgtcccccc cctggcaaac
    44341 aatactcaga taccgcccgg gggcaggccc ccgacgacta atcctcaaat cctacagttg
    44401 gggcaagcca cgggggaacc aagggacctg gcagtgtact caaccaaaac aatctttctt
    44461 gcccttaccg gaataagcac agagctcggt ccattaaatt tatttcgtat cgccaagggt
    44521 cgggttcccc ttgagcttgc cccactgcaa gcttagagaa ttagtattgg gggcctgccc
    44581 cgggcagcat ctgagaattg ctggtagggg cctgccccgg ggcagaatct gagaattgta
    44641 gacgggggcc tgccccgggg cagaatctga gaattgtggt cgggcctgcc ccggggcaga
    44701 atctgagaat tgtagccggg ggcttgcccc ggggcagaat ctgagaattg tagcctgggg
    44761 cctgccccgg ggcagaatct gagaattggt cgggggcttg ccccgggcag catttgagaa
    44821 ttagtagccg ggggcttgcc ccactgcaag tttagagaat tagtattggg ggcctgcccc
    44881 ggggcagaat ctgagcattg tagccggggg cctgccccgg ggcagaatct gagcattgta
    44941 gccgggggct tgccccgggg cagaatttga gaattgttgg tcgggggcct gccccgggta
    45001 gcatctgaga attgtggtag ggggcttgcc cctgggcagc atctgagaat tgtagccggg
    45061 gcttgccccg ggcagcatct gagaattagt attgggggcc tgccccaggt cagaatctaa
    45121 gaattaatac gagtgggaag ggggccggaa tacaacccat atccgtaaat tgtcatacac
    45181 ccagcacata tcgcaccaca tggagtgatt gattttcagt aattttgtac catgtttcga
    45241 aggttggctg aaagttccat ccctaccaga tggggagaat ttaaagtcat tgcctacggg
    45301 ataaaggatt ccgatcctac cccgcatatc gtcctggtaa aagaacccat ccccgaagat
    45361 aaaccggtat tgacccgcat tcattccgaa tgtgtaacgg gggatatctt tggttcacag
    45421 cgttgcgatt gtggtcccca actggataaa tcactggaaa tgatcggcaa atccggtggg
    45481 gtgctgcttt acctgaggca ggaaggccgc gggatcggac tcatcaataa actcaaggcc
    45541 tatcaattgc aggatcaggg gatcgatacg gccgccgcca atatccatct tggatttaaa
    45601 gtggatgaaa ggacctatga agacgccctg ctgatcctcc gggacctggg cattcacgag
    45661 attgaccttc tgactaacaa cccggataaa attaaagcct tcgaacaaag cggcatcatt
    45721 ctccatcagc gcataggcct ggagatcgaa ccggtccgtt ccaaccggta ttatctggag
    45781 accaaaaaga atatcatggg tcacctgctt gacctcaaat gaggacccga tgccggtttc
    45841 gcgcagttta tggcataccg acgacaccca ttctgaaatc cgcagccagg agctggacct
    45901 tcaggcggga catcagctgc ttcgatcgct ctattcctta atcagcctgg gtacggaaaa
    45961 aacgattgct ttgggtggtg tgccacccag gattttcgat aggatggaag tcccttacat
    46021 ggaaggcagc ttttcgttcc cggtcaaata cggctattcc ctggtagctg tggcggagga
    46081 tggccgctgt taccatttga tgcatccgca ccaggaccgg gtcccggtgc tcccggaaag
    46141 cctgacccta ttgccctcag ggattccccc ggcccgggcc accctgatca gcaatatgga
    46201 aaccgccctt actgcttatt gggatgcgga accggtgaag aaagaaaaaa tactggttgc
    46261 gggatttggg cttctcggcg ccctggtggc tttgctgttg cgtctgaaag gatttgaaga
    46321 gattaccgtt ttcgaaccgg atcccctgcg ttccggcatg gccaggagtt tgggcttccc
    46381 ctccggcgat cccggttttg atcccggtcc gtttgacctg gcctttcaca gcagcggcaa
    46441 tcccacggga ctgcaatcct gcctggaagt catggggatc gaaggccgca tcattgaact
    46501 cagttggtac ggcttccaaa agagcaccct ggccctcggt gaatattttc atacccgccg
    46561 gctgaggatc ctggcttcac aggtttcttc cattccaagg aaaatggcag gcgaatggaa
    46621 ttttgccagg cgcaagcagg aagtcctggc cttgctggcc gattcctgct gggataaact
    46681 ggaaattgaa gaggggccct ttgaaaacag cccggccctg tttgataaaa tcaggctttc
    46741 ccaaacaaaa ggcttaactt atatgctaaa gtattgaacc atgtacagcg tcaggatcag
    46801 ggatcacatc atgattgccc attccctgcc tcatgagggt ttcggcgtcg ccagcggcct
    46861 gcacggggcc acttatatcg tggacgccac ttttttcagc ccggtactca atgaaatgaa
    46921 tgtggtggtg gatatcggaa tagctgccgg agtgctgaaa aagatccttt cccatttgca
    46981 ttttaaaaac ctggacaccc tggacgaatt caagggcagg atcaccacta cggaatacct
    47041 ggccgcccat attcaccgca gcctggccaa tgaaataaaa ccatttttca acggcaggct
    47101 ccggatagag ctgggcgaat cgcatgtggc ctgggcttct tttgaaggag aaaccggttc
    47161 atgatctatt atgcctgccc cgatccactg gcattcgttt ccgggggcaa tctgctcaat
    47221 gcccaaatat tccgcgcttt ccgcgaagcg ggcagggcat ttgcccgtat cggctggcag
    47281 gatatccctt acatagattt tgctccgaaa gatttcctcc tcgtggattc catctggata
    47341 aacaagatcc aaatccgcga tttcagtaaa ataaaggcga ccaaaatatt tttcacccac
    47401 ctcttgcctt ccatgctgga tctcggcatc aaaaggatac aggagatcga gcggctctct
    47461 tgttttgatt tcctcctggc caacagcgcc tttacccgga actggctgga tgccctgatg
    47521 tccgggaaaa gcaggatcaa aaccgttcag ccctggatca aaaaaacagg aatgcaagag
    47581 agcgccgttc gggataagtt tatcctggtc gctgcctggc agcctataaa acaaatagat
    47641 cttttatttg atactttgac gcgcgttgat ttgccgaaag ggttcaggat ccattgttac
    47701 ggcgaccgcc aggtaaaccc atcttatacc tttaaatgca tgcaaatcct ggaaaaacat
    47761 cccggacttg cggagcatat cctccttgaa ggcgtggtaa gcgctgagca cctcgcctcc
    47821 gtctatgtag ccggcaaatg cctgatcgat tcttcccggt ttgaaaccta tggcatggcc
    47881 ctggccgaag ggcttgtcaa tggcctgcag gtcctggccc ttggtcaggg caatgtaatc
    47941 catttgatcg ggcagggcag aggcaagtat tgcagggacc tggaggaact ggtgcaaacc
    48001 gccgtattga tctttgaaga caagttgagc cttgaagaaa agccggtgca ggatatccta
    48061 acctcctggt ccgcttttgt cagtcagttt tcattcctgg atcagatcgg gcggatttga
    48121 tcattcgatc ccgtatggca aatacgcggg gatccggctt ggagggagcg aatccacttc
    48181 ggcccaggcc tgcatgaata aagaataaaa gccttcatgc tttaataaag atcctttttt
    48241 gaaaacgggg gccatgcccg gttcaaaccc cctggctgta aagaccctga cccagcgcgg
    48301 gtccttgctg atcaggtgga tgggggtagc gtaggtgtcc gtggctccat tcaggagata
    48361 ctccatgccg gcgggcgggt gatctccgat taatataaag agtgaattgg agccggcttt
    48421 ttccaggatg tatttttcca aaacggaaag atcataaatc actccctggg tataacgttc
    48481 cagggggctg ccgtcctccg cccggatccg tttatgcgga cttttttcga tctgatccag
    48541 cgatttccag tcatccagca ggggaggagg gtggtaaaaa ggggcgtggg tattcagggt
    48601 gataaaaaac aggaaaaagg gcgattgatt tttgtccagg acgtattcgt cccagtaatt
    48661 cagggcgtat tgatccggaa tgcctccgaa ataatcgtag cggtaacccc ggtatggaag
    48721 gtcggaatac cgggtccagt gccggtgatc aaaaaagatt tgggtgatgc tgtccagttt
    48781 ttcaaacgtc tctccaaaat tggccatggt attcagccgg taggtctgat agccctggtg
    48841 gttgagcgcg cggatcaggt gggggtactg ggggaattcc cggagcagtt tttcataagc
    48901 ggggtgattg tcaatccgga caccgctcat caggctggtg aacccaagcc aggagcggcc
    48961 tccaagaatg gtggaattgc tcagggctga agccgaatgc cagccctggc cggccaagcg
    49021 cttctgcatg gagtccagtt tgaggagata ttccctgttg taaggtttta ccacaccggc
    49081 tacggtacca taagcctcaa taaaaagcag gtagatgttt ggtttggaaa ccagcgggag
    49141 ggaatcatat ttttcgtagt ccaggcgtcg atgaagatcg gggagggatt tgaccctctt
    49201 gctggtaaag gcaaggttca tatcccgggc aatccagtga atggccggat aatacccgga
    49261 agcatcaaca ggcttattgg tataatacag cccggcggga aggaaaaata caaagcaggg
    49321 aatgagccag gcctttaaac cggggttttg ggaaaaccag ccggacaaga cccggtggcc
    49381 ccggtaaacc atcacataaa acaaagccag aagcagggcg agcaaggcat atgcaagaaa
    49441 aggagggatc ctcatcgatt tcataaatac aggcaacacc cttttaatca gggcccagtc
    49501 ataggtccat acggggatct ctccatatac cttccaagag aaatgaaagt aagcctgaaa
    49561 caggaacagc ataagataaa gaaaggccgt accggcggcc atccatttct gccggctcat
    49621 gctggccggc aaagccaaaa gggccagcac aaagccataa tccaggctcc accggaagac
    49681 atccggattc tggcgactca ccaggttgct gaaagattcg atcgcgggta aaaaacttcc
    49741 ttccgcctgc ttggccaggt aaagaggcag gtataagagc cattgcaggc aaaaaaggat
    49801 ggacagggcc ggcaagaaat ttaccctgcg attgtttttg cccaggcctg aatatcccgg
    49861 atggtccatt tccacaggat attcagcgat ttaagttcaa taatccgagc tccgcaaggg
    49921 cgccccggcc aaaagaaata aacaaagcgg cggcggcaaa gaccagcacg ggaaaggacc
    49981 atgcataagg cagaaaataa caaaggagga tgcttacaaa aaccacgacg gcaatgatcc
    50041 gcgcatattt atccttgatt tccttgcgcc ccggttttaa aaaataaaaa acgacgataa
    50101 agtacagata ccggatgagc ccaagcgata cgatccaaac cccggtgaat cctttttgaa
    50161 aaaggataaa agtcgtgcac aggacaaata aggcatccgt ttccttatcc atgtattccc
    50221 cgatggtgct ggtcatctta aaggtcctgg cgagataacc gtccagcagg tctcccaggc
    50281 aaatcaaggt aaagaggatg ccgagcaaca ggtccggaat caccggatgg atcagggtga
    50341 ccgcgatgag gccagtcatt cgcaataagg tcaccgcatt ggccggcccg taaggaaaat
    50401 ggagcgcccg gtgatttaaa aaatatttgg ccacccaata ggcaaagccc ggcaaggcgg
    50461 tgatcgccag gtaaaaaaca tcccgtttca ggatggaaac aaaggtgaaa aagataatcc
    50521 aaagggaata caggagcagg tcgaagtgca atttggtcag ggcctcattt tcctgcggtg
    50581 ggatcatagc ctgtggttaa aaggacatca tggacgggtt cgctcagggt caggccccgg
    50641 cccgatggtt tattttttct ttgagataat ggccggtttt ggaatctttc accttgacca
    50701 ggtcctccgg cgtgcctgcg aaaatcagtc gcccgccttc cttgcccccc ccggggccga
    50761 gatcgatgac ccaatcggcg gatttgatca catccatatt gtgctcgatc aggatgaccg
    50821 aatgaccgtt ttccaccagg gcattgaagg cgtccagcag tttgcggata tcgtggaaat
    50881 gcaggccggt tgtgggctcg tcaaaaataa agaggatggc ctcctggcct gcttcgcggg
    50941 tgaggtagct ggcaagtttt acgcgctggg cttcccctcc agatagagtg ctggaggcct
    51001 gtcccagttt gacatatcct aggcctacgt caaaaagggg tttgagtttt tgatggatgt
    51061 ccggctggtc cttaaaaaag gtgagggctt cttcgacact cagttcaagg atgtcgtaca
    51121 tcgattttcc attgaatttc acttccagga cttcattttt aaaacgttgt cctttacaat
    51181 cttcgcacaa caatttgacg tcggccagaa actgcatttc caccacgatt tccccttctc
    51241 cctgacaggt ttcacagcgc cctccttcca cattaaaaga aaaatggccc gggccataac
    51301 ctctgaccct cgacaggtgc tggttggcgt acagtttccg gatcgggtca taagctttta
    51361 cataagtgac gggattggag cgggtggatt tgccgatcgg attttgattg atcatctcca
    51421 ccccgcggat ctgtcgcagg tcgccttcca gggactgcaa tccggggatg atgccgaaat
    51481 ggccgtcggc cagttgcctt tccatggcgg ggaacaaaag gtgtttgacc agggtggtct
    51541 tgccggagcc ggagaccccg gtgaccgcgg tgatggtatt gagcgggaat ttgacttcca
    51601 ggtcctgcag gttgttcagg gtagctccct taagcaccag tttgtctttc cattttcttc
    51661 gccaggatgg gacctcgatg gattggctgc ctaggagata ggcggcggtg aggccgggct
    51721 ggttgggttt tataaactgt tcgtagcttc ctgtataaac aaccttgccg ccgtggatgc
    51781 ctgctgcggg cccgatatca atgatgtagt cggcctgccg gatcaactct tcttcatgtt
    51841 ccaccaccac tacggtattg cccaggtcgc gcaattgttt aagcacttcc accagccggt
    51901 tggagtccac cgggtgaagc cccacactgg gttcgtcgag gatgtaaagg ctgctcgtaa
    51961 gattgctgcc cagggtccgg gtcaggttga tgcgttgggt ttccccgccg ctgagggtcg
    52021 atgccagccg ctccaggctg aggtgtttaa ggccaagccg gcacatgata tccagccttt
    52081 gcagcacctc cctcaggatc cttgaggcga tcttttcatc ctgcttcgaa aatcggaggg
    52141 cgcggaaatg ttcatacagc tcatctacag gcaaatggat caggtccggc agggcaatcc
    52201 cgttgatctt gacataggcc gcctcctttc tcagccgtcc cccttcacat tccgggcagg
    52261 tggttcttcc cctgaacctg gccaggatga cccggttttg aattttatag gttttctctt
    52321 ccagctcccg gaaataggcg tcgatcccat agaaataatc gttgccctgc cagagggttc
    52381 ggtattgttc cttggtcaat ttatggatcg gcttgtgtat gggaaagtca aagagatggg
    52441 ctttctggat caattcctgg gtccacaggc tgcctttttc tccgctccag caggcaatgg
    52501 cgccttcata gaccgatttg gtcttgtccg ggatcacctt atctgcgtcg atgcccatga
    52561 cccgcccata tccttcgcac cgggggcagg ccccgtaaga gttgttgtaa ttgaacaatt
    52621 ggggagtggg ttccaggaat tggagcccgt cggcctcaaa acggttgttg aacaaatggc
    52681 ttttcctggt ttccgaatcg tagacgatgg cattgccttc gccttccgca aaggccgtga
    52741 gcaccgagtc ggccatctgg ttgacaaaat cctcgtcttc cggattcgtt acaaaacggt
    52801 cgatgaggat ggcaatatgg gcattgccca gtttggacag tttatctttt aagggccagc
    52861 cttttgactt ctggacctct tcgatctgaa aaagttcatg gttccgcagg atccgggaat
    52921 accctttttg caaaaggagg gcgagctcct ggcccagggt acggtcatcg tatttggtgg
    52981 agagggggac cagcaggtaa atctttgatc cggctttcag gtttttgata tagtccacca
    53041 catcgcgtac ctcatgcctt ttgacgacct ccccggaaac ggggctgtaa gtttttccta
    53101 tccgggcgta aagcaagcgg agataatcat agatttccgt catggatccc accgtcgaac
    53161 gcgcgttgga ggtgcttact ttttgttcga ttgcgatggc cgggcagatc cctttgatat
    53221 aatccacctc cggttttttc atccgcatca ggaactgcct ggcataggag gacaggcttt
    53281 cgacgtacct cctttgccct tctgcataga gggtgtccat gatgagcgag gatttaccgg
    53341 accccgacac cccggtcacc aggaccagct tgcgtttggg gatccataca tcgatattgc
    53401 ggaggttgtt ggacctggcg cctttgatat agatggaatg ccgggtgtct tcggccaggg
    53461 gaggggatat agcctcttcc tcttctttgg atctgatttt caaaatgcct tgcttgtcaa
    53521 aaaaacaccc aaagataagg aggagggcct tttttttcag gtaaaatggg aatccggaca
    53581 gcaacgaatt ggggagaaaa cgccgcttgc cgggaatgtt aaaaaaacca aaaaatttta
    53641 aaaaaatcca gcgcgcccat aggtggaata taatttgatg tatatttggc cttattcact
    53701 gttaaaaatc gcgcctatta gagacataac ttctacacta taatttattt aaaaaccaaa
    53761 attattatag ttatgcaagt tatgttgctc tctgatcagg atttgattca gcaatatctc
    53821 aacggcagtg aacaggcctt tgaagtcctg ctgaaccggc acaagaccaa aatcttcacc
    53881 tccatctacc tgtttaccag ggaccatgaa gtcgccgaag atattttcca ggaagtattt
    53941 attaagatca tcgacaccct cagaaagggt aagtacaacc acgaaggcaa attcctgcaa
    54001 tgggcgcttc gcatctcata caatatgtgt gtggaccagt tccgccggtc caaacggcag
    54061 acccaggttt cccccagcga gacctttgat gtcctcaatg tgatccaggc gtccgacgac
    54121 aacatggaac aaaccatgat caagtcgcag ggcatccacc ggatccagtc cctggtggac
    54181 caacttcctc ccgagcagcg cgaagtggtc atcctccgcc actatgccga tatgagcttc
    54241 aaggaaattt cccaactgac ccgcgtgagc atcaacacgg ctctggggag gatgaggtat
    54301 gccctcatca atctccgccg gctgatggaa gaaaaggtcg aactggttta ttaattcaaa
    54361 aggcatgggg gcggggcctg ggacaagggc cctgcccgat gcatccacct gcacgcttcc
    54421 cctttctctt gatcgactcc gccgtattct cttttaaaat ttttactttt gcgccctaaa
    54481 acgaatcgaa ggcatgggtc gcatatttga agtacgcaag tccaagatgt ttgcccggta
    54541 cgaccggatg gccaagcagt ttactcggat aggcaaggag atcatcatcg cagtcaaggc
    54601 cggggggccg gacccggatt ccaacgcgca gttgagaaga tgcatcgcca atgccaagtc
    54661 cgtcaacatg cccaaggacc gcatcgaagg ggcgatcaaa agggcgctcg gcaaggatac
    54721 cgaaaattac gacgaggtct tgtatgaagg ttatggaccg ggtggggtag ccatcctggt
    54781 cgaagcggcc acggacaaca atacccgcac cgtagccaat atccggagct atttcaataa
    54841 aatgaacggc agcctgggca ccagcggttc ggtcagtttt atgtttaaaa aaatgggggt
    54901 gttcaaactc caccccgccg gcctcaaccc ggaagaactc gaactggaac tgatagactt
    54961 cggcctcgaa gaactgggtg aaggcacggg ggaaaacgaa gagcccatcc ttgtcctccg
    55021 ggtccctttt aatgaattcg gcaccatgca gaaagccctg gaggaccgga agctcacccc
    55081 gatctcttcc gagatggaat ggatccccct gaatacgact tccctggatg aggaaagggc
    55141 cgaagaagtg ctaaaactgg tggcaaaaat cgaggaagac gaagacatcc agaaagtgtt
    55201 tcacaatttg gcctgagaac ggttttgaac ccatcggaga gcgggaccaa tagccgggtc
    55261 aagatcccaa tttaaatttt cataaatacg gggcaaggaa gccctgacta tccgtgtcaa
    55321 ggggggcaga tttggcctgg aattgatttg gaatttgtcg tggattcaag ggaatctcat
    55381 ttggaagtga atgctaaaat gcccttttct ggggtaaatc aatcagatga tcaattgggt
    55441 ctttgcaaac tggcaccagg aagggtttaa catgtttcat ggataggttt aacatattaa
    55501 agcatagaat ctattatttt acttaggtga aacggatatg aaatgaatac tctatatttt
    55561 aaggttagcc taatattccc tttatttggt ttgtcgttcc ttgcaaacgc tcaacatttt
    55621 tacctcggcc taggttattc atccatggac ccaaaatcaa gttttcagtc aaggtattat
    55681 cgggatgggt accctccaaa acttgaattt gaatattcga taaatgcagg gtatgaaaaa
    55741 aaatttggga aagtcctcgg tctctccctg tatttgggat atgtgcacaa tacgaataaa
    55801 aatgtcttct ggattagtcc aacgtatttt ccgcagattg atccccggaa gaccgtattg
    55861 aggtactcat tcaataatat cagtcattta ttctcggccg gagcatccgt caatctggac
    55921 ctcgtaaaaa tcaaaaacag cgcctgggga ctagcgccct attttaggac gaacttctta
    55981 tttaagaaac agacccaacc ggttgatttt gatggtttta aatttacaac acataagttt
    56041 aagcctttta gtatggaagc ctatccttta ttgttctgca ggatcgaccg ttttctgatt
    56101 caagtggggg ccaggatgta ttctttaaaa tactttgatg aggtcgtgga ggtaaattat
    56161 tccaacccga acccgaaagt ggactttagg aattcgttta aatggatggc cactgtttct
    56221 tacagattat agaaaattat accggaagga gatcctcatc ttatttttgg acgatgtgcc
    56281 aatactattt gatttgattc gagtggttag tggcagtcca atcaggcaat ctggtgtctg
    56341 aagtttcctg gtataaacag taaatttttg acagaaaata cccccttgag gcaccacgtc
    56401 ctatacctac gaccgtctgg accgcgaagt ggtcaggtcg gatcccaacg gtttccggat
    56461 attcaccaaa tacgatatct taggacgggt gatcatatcg ggaaaacata ccaccagctc
    56521 aaatccaact ggttcggagc agctctatga aacggcttcc ggctcgggat atttttatac
    56581 ctcat
//
LOCUS       DAOQXT010000019        56331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_595_length_56331_cov_8.517485, whole genome shotgun sequence.
ACCESSION   DAOQXT010000019 DAOQXT010000000
VERSION     DAOQXT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 56331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56331
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_595_length_56331_cov_8.517485"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..995
                     /locus_tag="PKM27_05005"
     CDS             129..995
                     /locus_tag="PKM27_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNR06654.1"
                     /translation="MSQEKTAIKTTYFSIAGNALLAIIKWLTGYFGNSYAMIADAIES
                     TTDIFASVLVLMGIKYASKPADENHPYGHGRAETLVTFIVVGFLMVSATVIAFESVRH
                     IRLPHEAPESYTLWVLAAIILWKELSYQWVIRKSRETNSSSLKADAWHHRSDAVTSVA
                     AFIGISIALIGGEGYEAADDWAALFASAFIIYNAWLIFRPALGEIMDEHQYDDLIARI
                     RDVAATVEGVKGTEKCFIRKAGMKYLVDLHATVEAEIPVRQGHEIAHRLTAVLKKEIP
                     LIGYVLIHIEPE"
     gene            complement(1027..2397)
                     /locus_tag="PKM27_05010"
     CDS             complement(1027..2397)
                     /locus_tag="PKM27_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR06655.1"
                     /translation="MEPFNINRRRFLQSSTASLAVSAFGLPNLDLFYPAKNYRVALIG
                     AGWYGKSDLFRLIQVSPVEVVALCDVDKNMLDGAAKLVSQRQKSGKTPKLYGDYRKLL
                     AENELDLVLIGTPDHWHALQTIDAVKAGAHVYVQKPISVDVIEGEAMVAAARKYNKVV
                     QVGTQRKSTPHLIDAKKNIVDAGLLGKISHVEMCCYIHMRNNGNPPVEPVPSFLDYEM
                     WTGPAPLRPYDGLPHLRWWRTFMEYGNGIMGDMCVHMFDTVRWMLKLGWPKKITSAGG
                     IFVQKGGKSNIADTQTAIFEYDELHCVWQQRSWGNSDNPDYPWSFTLYGEKGMLIAST
                     MRCDFIPVDPKGQKIHLDVVYEKEKYPEDLTEERIELNAAPATRLHMLDFIAAIENKT
                     RPVADIEEGHISTASCILANHAMETGRAMRYDPVKRIVKDDAEATKLLQRPYRKPWIH
                     PDPLKV"
     gene            2628..2960
                     /locus_tag="PKM27_05015"
     CDS             2628..2960
                     /locus_tag="PKM27_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR06656.1"
                     /translation="MAYTKKEEFGAREQALAAFAKALSHPARISILGILSKKEECICG
                     EIVDDLPLAQSTVSQHLKELKEAGLILGETVGPRSCYCINWKAFERFYSEFSQLFHKL
                     KEQQGRCC"
     gene            3228..3986
                     /locus_tag="PKM27_05020"
     CDS             3228..3986
                     /locus_tag="PKM27_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNR06657.1"
                     /translation="MERAGEDHSKTVGNGYSQLTANGAIRKPFYLSHDEPFSIEDPPA
                     NSSAQTRAELDFLLELQSRRTEEDIRASKYFASVFYRTSTRPGDADYERMQRNLFHIG
                     RSVAPWFNADSLPATAELVRRVWADASYILWKCKNHFVRIRPYKLDPALKNLEETNWA
                     AYPSGHATNSYTNAYLFSELMPEFSSFFIKDAYDMAHSREILGVHYPSDSESGRFLGY
                     ELIQKLKTNPVFRADFEKAKLEIQSMKKRKGYGS"
     gene            4551..4799
                     /locus_tag="PKM27_05025"
     CDS             4551..4799
                     /locus_tag="PKM27_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06658.1"
                     /translation="MKVYPNPVFNILTLHWEPSEAHYADLIISNLDGKVVLKQKVESV
                     TTPDHRLDLKILGLSPGMYLISLINRDSIVSKRVILTG"
     gene            complement(5299..5745)
                     /gene="tnpA"
                     /locus_tag="PKM27_05030"
     CDS             complement(5299..5745)
                     /gene="tnpA"
                     /locus_tag="PKM27_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNR06659.1"
                     /translation="MSTNTQIFYHIVFSTKYRERTLHADGRPALFRYITAILKNKKCH
                     MYRINGVEDHLHMLMDLHPSTSLSDLIKDIKLASSKFIKENKLFPSFGGWQDGYGAFT
                     CSYREKDALIAYVNNQERHHQKKSSMEEFIEFLNDAGVEYDEKYLG"
     gene            5958..7931
                     /locus_tag="PKM27_05035"
     CDS             5958..7931
                     /locus_tag="PKM27_05035"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNR06660.1"
                     /translation="MHRFLLFCFIFPVHLPCSIAQKSLVLHSPDDHLRFVIRVSDGML
                     QYEIDYKGKPILQKSNLGILLGNGNDLSQNLKTRKIKTREGLDEYELIVGKTHRVREP
                     YREGIISIRSRSRPKWKFDLISRAYNGGIAFRYVFPDQKSGDTLFLAGESSCFNYFGD
                     PRSMVLLRQNFITSHEGLYSHESLSRIQEDTLIDLPALFITPDSIYTAITEAALSDYA
                     GMYLKKRKGILTSALSPLPNKAEIKVKSPLPHQSPWRVILVSDRMGDLIESNILTNLS
                     PPNKIKDPSWITPGKTTFPWWNGNILPDSIKGGNNFETNKYYIDFCARNGIEYHSIVE
                     YGGHQWYVDDGEVYQPGPNADVTRPVPGLDMKRVCDYAHAQGVKPRVWVHWAALYPKI
                     DTAFALYESWGLTGMMIDFMDRDDQEMVRIQEEMLEKAARHKLHVQFHGAYKPTGMSR
                     TWPHELTREGTLNYEVHKWDSTVTPDHDLDLVFTRMLAGPADCHLGGFRAVPRDSFKI
                     QYIKPFVQGTRCHLLGMYVVMESYLQILADYPDAYEGEPGFDFLREVPTSWDETRVIA
                     AAPRQYVVIARRKRDFWYIGAINNREDREISIPLSFLPAGNHTLDIYTDAGDVEYLPN
                     HLHREIKKGSRTDILKIRMGPGGGMAGKASIDQ"
     gene            complement(8121..8537)
                     /locus_tag="PKM27_05040"
     CDS             complement(8121..8537)
                     /locus_tag="PKM27_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06661.1"
                     /translation="MMKKIKKYMIRTLVVLLLLLVAAGAFFTLVPYSDGMRAGQVIKL
                     TKKGVIFKTWEGELNLLLMPSTNTQGTLTPNVWNFSVLNKDIANKVMQASATGERLEL
                     YYKERYFKFPWQGDTRYFVYEVKSAPPPAQQVPSTR"
     gene            8593..9984
                     /gene="mnmE"
                     /locus_tag="PKM27_05045"
     CDS             8593..9984
                     /gene="mnmE"
                     /locus_tag="PKM27_05045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNR06662.1"
                     /translation="MYNRHSLDDTIAALSTAPGQAAIAVLRVSGAEAIPIVNEGFKGK
                     NLEKTESHTAHLGFLMDAEGGVIDQVVVTLFRGPHSYTGEDTVEISCHGSTWIQQAIL
                     NRLIQLGARAAGPGEFTFRAFIHGRMDISQAEGVADLIAAESSAEARLALSHLRGGIS
                     KEIAGLRSRLIEFASLLELELDFAEEQVEFANRSQLKTLLEEAILKVQGLVDSFRLGN
                     AIKQGINTVIAGRPNAGKSTLLNALLQEERAIVSDIPGTTRDTIEEVLHIEGIPFRLI
                     DTAGIREAQDQIEAIGVQRTHDKIRQSSILLYVFDVTGLKPAEVQSDIAVLKHPDLEI
                     ILIPNKMDLNPYAHPEDFVMEGITPSDIIPTSARNNMNIPYLKERLLQSVTGNHDLLS
                     QNILSNSRHLDCLQRVKEHLRRALEQLNSGGGHELIAMDIRQALHYLGEITGEVTTED
                     LLESIFTRFCIGK"
     gene            10191..10484
                     /locus_tag="PKM27_05050"
     CDS             10191..10484
                     /locus_tag="PKM27_05050"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNR06663.1"
                     /translation="MVVISKTKLTDFGRRHAEAVEAVNDWWEKTKEADWASLADIKRT
                     FNSVDYIGNDRYMFNIKGKKYRLVAMIFFDIRTLYIRFIDTHDEYNKKDCTTI"
     gene            10487..10876
                     /locus_tag="PKM27_05055"
     CDS             10487..10876
                     /locus_tag="PKM27_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR06664.1"
                     /translation="MINKIRSEKEYKAVMKTIETLLEKATNSGGFHLLPKEESAMLAQ
                     FSKIAEEYEDHAMQLMPIKPKTIQEAVEFKMAEQKLTQAKLAKKLGIGAPKLSQILNG
                     KREPDVVFLKAVYQKLKIDAGFLLTHV"
     gene            complement(11333..12007)
                     /locus_tag="PKM27_05060"
     CDS             complement(11333..12007)
                     /locus_tag="PKM27_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR06665.1"
                     /translation="MEIALQIKMNEKLFLRNPELSALGKKIIQHSIQLIHKNGFESFT
                     FKKLAADIGTTEASIYRYFENKHRLLIYIAAWYWSWLEYRVSVNTMNIKDPAVRLKRV
                     IKLLTAVVEDDIKTTYFNEDILHQIIIAEGTKAYLTKHVTEDNKDQLFKPYKDLCAKI
                     GEIILDYNPKYKYPKSLASTLIEVAHFQNFFMHNLPSLTDFGKDKDESKIVAFLEDLV
                     FSSIKN"
     gene            12170..13720
                     /locus_tag="PKM27_05065"
     CDS             12170..13720
                     /locus_tag="PKM27_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNR06666.1"
                     /translation="MEIKNIFKDFKNDLPASIVVFFVAVPLCLGIALASGAPLFAGII
                     AGIVGGIVVGMASGSPLGVSGPAAGLAVIVLSSIAAIGSWPAFLLAVVLAGILQLIMG
                     FAKAGFIAYFFPNSVIKGMLTGIGLLIILKQIPHALGYDKDPEGDLSYFQTDGGTTIS
                     SISNAINALSPGAILISTIAIAILILWDTVLMKKHKLFQVIQGPIVVVVLGIAMNYLY
                     QTGALNFSLDADQVVKLPVANTLREFFSFFTFPDFSAITNFKVWEVAVVLAIVASLET
                     LLCVEATDKLDPYKRVTPTNRELKAQGLGNVISGMIGGLPITQVIVRSTANITFGGKT
                     KMSAILHGLFLLISAITVAEWLNMIPLASLAAILIIVGYKLAKPALFKQMYKLGWEQF
                     VPFVATVVGILATDLLKGITIGILFGIFYTLRHSYRNSHHLKDVVTTREGQQVHHIEL
                     AQEVSFFNKASVMRTLEAIPAQSKVIIDFSKSKSVAHDVIEIIQNYRINAKAKNITVE
                     TVNFIEPA"
     gene            13761..14414
                     /locus_tag="PKM27_05070"
     CDS             13761..14414
                     /locus_tag="PKM27_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNR06667.1"
                     /translation="MKTLTKEMQAAITPSTALNLLKEGNKRFVSNLKINRNLLQQANE
                     TSDGQHPFAVILSCIDSRTSAELIFDQGLGDVFSVRIAGNIINEDILGSMEFGCKVAG
                     SKIIVVLGHTKCGAIKGACDHVEMGNLTALLSKIQPAVYDEKSVSENRNSGNPDFVEK
                     VATLNVKRTVTAIMERSPILKEMIESGAIGIVGGTHDITTGEVKFYEDTLIINKRGE"
     gene            complement(14431..17100)
                     /locus_tag="PKM27_05075"
     CDS             complement(14431..17100)
                     /locus_tag="PKM27_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR06668.1"
                     /translation="MKEQPQHQPPSWARKFIEWYCKPELAEDLIGDLNEYFERNLRSV
                     GPRRAKLIYMIDALKFFRPYTVQKPKWVNPFISRIMIESYIKTSGRGLMRHKLFSFIN
                     IMGLAVSMSVGLLLIAFVFDLFSFDRFHKNGERICRITNVATFNQGPGGKFATTSVKT
                     GKLIREKMTGIEEVVILQNDFSQDAVVGDRILPLKGYWAEPSLFRIFTFPMVEGNPET
                     ALKDPYCIVLTESAAKKLFGGQSALGKTIKFDSLPYQVTGVMKDVPFFSHISFEALVS
                     FSSVEKANQHDPRFEAWTNMWSNQVYLLLQENADRVSIQSQLDAIAREENLVQGPEGY
                     RDTRIRLELLPLHKIVVGEQLSRSMGPSGPHVSPALLWILVGLAFVVIGSACFNYANL
                     SVARALRRFREVGLRKAIGAGKSQVRHQFLVEAIMISLAALLVSSLLFFLLRPQLIRL
                     SPEMQNMVKLDLSPAMVAAFIFFSVTVGVIAGFVPALYFSKINASHAFRNGPPRKAFK
                     HLSLRRALVVIQYTLTLIFITATAVGYVQYKNILSFDLGFNTENILNIHMQGNKPDAL
                     LKELKEMPEVTGLSRSLIVTSVGNAWGGYMTYKNSQDSALVLTNNVDENYLPLHEYKL
                     VAGGNFIVRPTTAEAAREVIVNEQLLKRFNIAGNDPEKAIGQEITFSNFHVHRRKMTI
                     VGVMKDFHYGTVENVIGPVAFIPWAPEDRAIINAKIHSTDMQATLAKIESTWKKIDRV
                     HPFKAQFYDEEIENAYNEFAAIIKIIGFLSFLAISIASMGLFGMVVFTAETRLKEIGI
                     RKLMGASQGNLIYLLSSGFLSLLSVSALIALPLTYLFFQKVVLLRFPYHSPVQFPELF
                     AGFLAVLLIAIVLIGSQTMKAARSNPAVVLKSE"
     gene            complement(17105..17431)
                     /locus_tag="PKM27_05080"
     CDS             complement(17105..17431)
                     /locus_tag="PKM27_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR06669.1"
                     /translation="MKGTNLGEFEELVLLTIAALMDKAYSVAICDELSTHNGRTVKLG
                     VVHSVLHRLEEKGFVKSELGEATKSRGGKRKRFYQLTVTGKEALVRTKAMRDQLWDRI
                     PSLAWK"
     gene            complement(17817..21551)
                     /locus_tag="PKM27_05085"
     CDS             complement(17817..21551)
                     /locus_tag="PKM27_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNR06670.1"
                     /translation="MFVTGPSSRYLFVFPLALFFLLAFSSDDPTKLRVLVFSKTEGFR
                     HTSIDAGKLAFKQMSEEKGFDVDFTEDAGQFTANVLKQYNAVIWLNTTGDVLNNEQQT
                     QFERYIQAGGGYLGIHAATDCEYDWNWYGRLAGAYFLDHPNPDNVQKGKFQVVEKNWA
                     TQGMPDEFERLDEFYSFKQIDPTIKVLVKIDEKSYRGGKNGDHHPMSWYHDFDGGRAF
                     YTAMGHTDESFSEPLFLNHVWAGLQYAMSSEKPVILDYSKAKPEENRFSKVILLEKLN
                     EPMELSVLNDGRILFIERHGAVKIYNTRTKSVKTIATIPVSTQYKDREGKTSEAEDGL
                     LGLNKDPNFNVNHWIYLYYSDPGKSQNILTRYTLVGDVLDLKSKKVLLAVPTQREQCC
                     HTGGSIAFDKDGNLYLSTGDNTNPHGSNGYSPSDERPDRMPWDAQKSSANTNDLRGKI
                     IRIKPRADGTYSIPEGNLFAAGTDKTRPEIYTMGHRNPFRISVDKKTGFVYWGEVGPD
                     ANDADPARGPAGHDEVGQARVAGNFGWPHFVGDNKPYYKYDFAESKSLELWDASKPIN
                     TSPNNTGLEALPPAQKAFIWYPYAESKEFPLVGTGGRNAMAGPVFYSEDFKTAKRAFP
                     KYYDKKLFIYEWMRGWIMAVTMDQEGHYQSMERFMPGYRFSNPMDMEFSADGDLYLLE
                     YGSGWFSANDDARLVRIEYNGGNRKPEIRLVAGKLAGSVPFKTTLSSKESTDADGDTL
                     SFAWKITSKLGFIKNIVGPEVDLDLSRAGIYKVTLTAADNRGGISTQSVELIAGNEPP
                     VLKFDLGENNKSFYFPDKTYEYKVVVDDQEDGSTSNGKINPEEISVNIDYLAEGHDKT
                     IIAQGHRTADATAAFSKGMKLIAAGDCKTCHDVTKKLIGPTYRDIALKNKGKPGAQQV
                     LAKKIISGGQGVWGQVPMAPHPQLSAEDAAEMTRYILSLADDKPKVKSLPVEGKYTIK
                     LPDGDKGQGVYIFRAAYKDHGANSLPGILTEESFVLRNPGINPSDYEEFSDVNKMSFG
                     GNKFVIPTKPGSYVGLKDIDLTGLTQLEAMAMAPKAQLNAAGGYIEVRIGQPSGKLIG
                     KSEFIGDHGAGFSPKPVLINLEPTTGFHPLYLVFTNPELKKAGSLMILLNTRFKTGTV
                     DVKPKTLAPTGDLNAYTGKYRMTGLPFEFITISVKDGTLIIEANGQTGPLSPTATPHT
                     FDADGKALLKFQMDDQKAVTKMKMEAMGFSFEGAKVTGSN"
     gene            complement(21692..22882)
                     /locus_tag="PKM27_05090"
     CDS             complement(21692..22882)
                     /locus_tag="PKM27_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HNR06671.1"
                     /translation="MIIHWPTGNHAPFNGEGELPFLKRLNGSDWDGKLEYNILVQEAP
                     DFTTPNEKYWLFVDRFLAYCERKGLLVFMFPAYLGYNGEEQGWMKELVANGKEKTKAY
                     GAWIANRYKGRKNIVWMLLGDMGKFNDEQQMAEQALIDGLKSVKDQQSTQYTAESHSG
                     ENAADNIAFGHEMTLNGVYTWELKVPVPYLGRKAYAHDPPMPAFLLEEPYDEEGPDGN
                     NYNPNATQPVRRFQWWGWLSTIGGYISGNGYVWPFIDPLWQQHLNTRGARDMARLNRF
                     IKSVEWWKLVPAGLNGMKNFIQDPENIDTSASYIAAAAARDGTFLVAYFPPAHPGEVS
                     VDLQVLSKPGFAYWFDPTVGRYLPVQTSAFSNTGISRFVPPGKNQAGETDWALVVSVG
                     RINK"
     gene            complement(22879..23118)
                     /locus_tag="PKM27_05095"
     CDS             complement(22879..23118)
                     /locus_tag="PKM27_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06672.1"
                     /translation="MIKALAASILFSTVFHCVYAQAAFPLRISDDHRHLVDRKNKPFP
                     ILGRTAWCILSQPEKAYRKFVENTLAHGHNAFRSR"
     gene            23270..24235
                     /locus_tag="PKM27_05100"
     CDS             23270..24235
                     /locus_tag="PKM27_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNR06673.1"
                     /translation="MKAHLPILVIFAFILNACEKDQSGGDTEKIIYGKIRLTTKIDGD
                     DREYFVHVPKAYTGKQAVPMVFMLHGTSGDGEEFYDRSGWKEVGESQNVITVFPSSGR
                     YCIHTQGETNKTTKWNTAPDAEWTFCSGQAPKDDIKFLRTIIDELSSKYKIDAKRIYL
                     AGFSNGGQMAAKCSIELSDKLAAVVLSAGTFYLDTIYTPKRKLPVLYQVGNEDYGPGN
                     TGPAIPMNQFSYLISTPGLTFKDGRFNIIATDHVRNFALNPNFTISGDTTKASIATYK
                     PLLTTETHEFQYILIKGLKHAYPNGDNHPLEAAELNWSWMKQFSL"
     gene            complement(24336..25253)
                     /locus_tag="PKM27_05105"
     CDS             complement(24336..25253)
                     /locus_tag="PKM27_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06674.1"
                     /translation="MQNKSLGLIMMMIGLSALIYTARGQGCSDAGFCTLGNFKPNGLD
                     STSGFKNELKLGLSFGRADHSITIFGTHLDYQRMLNKKLSFSARLTSLSQSGNGISAF
                     GFSDLFLSADYPAGTIGKITLGFKLPLNDASKIKKNLPLPMDYQSSLGTLDLLLGISR
                     VWNKFHLALALQQPLAQNQNEFAAEDYPAGSALKKFQTTNRFRRRGDLMARVSYSIPL
                     NNQFKITPGLLPVIHLGKDQYTDREGLERAIDGSQGLTLNGTVHFDYEINNNHSLQLN
                     AGVPFIVRKERPDGLTRSFIATLEYRRTF"
     gene            complement(25370..26581)
                     /locus_tag="PKM27_05110"
     CDS             complement(25370..26581)
                     /locus_tag="PKM27_05110"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNR06675.1"
                     /translation="MNKSNCMFLVAVFLMAVSCESTAQPGKANASVETQNANTNYKPA
                     FIGQTRIGAVTTKTPYELKLLTSSLRSPWGIVSLPDGRFIITEKSGTMRIVSASGAVG
                     DPLDGIPAVNSRGQGGLLGLCIDPSFSSNRMVYWAFSEPLPEGNVTSIARGILSSDEK
                     SLEQVAVIYRATPAYDGALHYGGRVLFSKEGHLFLTTGERSDRATRPQAQDINSGLGK
                     IIRLTRDGKPAPGNPFAGRSGARPELYSYGHRNVQGIAFHPVTGDLWETEFGPRGGDE
                     LNRIVAGKNYGWPVITYGIEYGGSRIGDAIQQKEGLEQPVYYWDPVISPSGITFYNGA
                     EINEWKNNLFIATLSGGHICRLVIENNRVTGEERLFSGERQRFRDITQGRDGALYTIT
                     DQGRLYRIGKK"
     gene            26926..29109
                     /locus_tag="PKM27_05115"
     CDS             26926..29109
                     /locus_tag="PKM27_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06676.1"
                     /translation="MMCNHLAKYLKYKTILFYSVFFSLSALQSAFGQVSDTSTLTEAG
                     SPEPIWVLSPAGSMQKITEAPALVSLVSNDLTQKYGWLSVNEILYSLPGFAPSQDYDR
                     RTVSFRGVFEGWNKNHILTLVDGIPFNDNLYGSAYTWENSPLVFTKSYEISRGPGGSL
                     YGGNAINGTVAANTLEATDLDGIARVRLRAGSNKTLITDFLTGTENKSAGIVASFTRF
                     YTGGNEYESYDLSGRTRTNAGLEKFKIRDEQDNSYFFAKAYGKGKYKGLSLQYHEQHW
                     EFQTGHGWLFSVPDQPESMNEYRRVLAFKYSPGREGKRFNYEALARYQDHGVHWNMRF
                     FPDRSTAYGLDFPNGVSESLKTGAKDFFIRFQGTYSLDSSKFLFGLDGDRFYYRGDVF
                     HTANMDLNTFTAPDSSNRTYTLNPWMEYILDKPVHHAAAFVRYVSPMWFDKLQLILGG
                     RYEHRFFNYTDIQSAGRPIRHKSFNLFAPHASLTFHAGDLVLKAMAGRSLRSPSPTEM
                     FGANTFTLASNIKELEPEILTNFDLGLDWKWSESLRFGLNGFLFHFENQIAYSVANAN
                     LSTNIYSLKTAGLEAELHFTGNRLSGFFNYSFSKRLDESISDSTVAISRKKLTWAPAH
                     MANLGMVYSIPKFYAAIGTHFQGEVARRSSDILEAFEGYRPAAVSSWVNLDAKVAYKL
                     NPSVEIGLSAKNITGQGQYLVKNNAAPFDYRREGRRVFMDLSFRF"
     gene            29345..31354
                     /locus_tag="PKM27_05120"
     CDS             29345..31354
                     /locus_tag="PKM27_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNR06677.1"
                     /translation="MKYLFVILNLRLPTIKWAIFGVILPLLPGFPALAQKEIPAFRFG
                     KLPASNFIPKVCPIDSAAHAYYLFDHGQSTFLITPEVFKVVFERHFRIRIQDKSAFDQ
                     ADISIPLYYKDPKSEEKLVELKGITYNLENGELVETKLEKSSIFKEKTGEHQNQVKFT
                     MPNIREGSIIEVLYKIESDFLFNFQGWVFQHDIPVLESEYLTAIPEQLQYNPYFWGYL
                     KVNVSREERKKPGYDFFEILNGYLIRNVPAFPSEPYIHNKENYRTRVEFELASTKFGI
                     DYKEYTKSWAALNKLLLEDQQFGLQLSKTGYFKDEVAEIQANFSQPNDKINAAHQLIQ
                     QRMRWNGDHGIYTTGNIRKSFEQRKGNVADINLCLVSMLRELGLSAFPVVLSTIDNGA
                     ILPTLPTLTKMNYVLAAVIQGDKKILLDAADPFATPSLLPGKCLNGQGRLINDQDGVW
                     VDLKTSKIDQSQAYYKLDLDPQDGFKGTYQERHFNYSAYRMRHQLGDSTDLTEYLKNI
                     HEKHPGTELTEAIMTNFNDYQKDIILDAKIKTRGNMEVTDELIYFTPIQMEKLVDNPL
                     KIEDRQFPVEFEYPWLVNATVEIRIPESYDLESTPKSAIFQNASKTAKFTFGVEVVSG
                     NTLKISSLIFLTKAQYEVDEYEGLRDFFNLVVSKHAEQVVLKKKV"
     gene            31357..33216
                     /locus_tag="PKM27_05125"
     CDS             31357..33216
                     /locus_tag="PKM27_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNR06678.1"
                     /translation="MKLFYPFIFCLVSPFLYALPATEPDYITIHHSKTHYKINALDDV
                     EESVWKNYTIHHEKGLKEATLILPYDKQSKITEAEIMVKNKFGMITKYKLRDFTDQVM
                     QDGYSIFSDNRLKYLKIEATGFPMNIDFRYTIKYNSTLGLGRWMPQEGYHYRLEHAEL
                     TVQSEIPDLVSYKEVNFRGARQVESKSLAWVTDTLEAIQDEDFDTPMEEKFSMVLLAT
                     NQIEFEGHRGDLGDWNQYGKWVNGLIRDRDQLPPEDQLKINALVKATDSPKSKINTLY
                     KYLQENTRYVSIQLGIGGFQPMAASEVAKAGYGDCKALSNYMRALLKHVGINSYYTEI
                     GSGSRKINHTDFSSLYQTNHVILTVPGPEDTLFLECTNQYFPPGYLGNANSDRYALMA
                     CPEGGRLIRTPVYDEGSNTLTSHSELRLDAFGHGASFTTARYGGLFYETYADYDLKGE
                     KEKKEQLLENRKGMDYALDSFNYFFDYASVPRIAFNEYAHIKKYASVSGIRMFIPLQL
                     IHELAPDLIPGKSRMNDIVIPFGFSQKDTLSIVIPSAYEIESLPKPFTIKSVFGELDF
                     NATVMDRTVQIRLHFTLLKTRRSKEAYTEFSDLLSKASKVRQSKIVIKKIEKP"
     gene            complement(33317..34042)
                     /locus_tag="PKM27_05130"
     CDS             complement(33317..34042)
                     /locus_tag="PKM27_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06679.1"
                     /translation="MKIKTIIWLAVFSIAMGYLESAVVVYLRKLYYPDGFKFPLVPME
                     AEMAMVELLREAATILMLLGIGMLSARKASLRLAVFIFCFAIWDLFYYLFLWILLDWP
                     ESFFTWDILFLIPVPWVGPVIAPCILCLNMILLASAIFYYHSQEKDTRLKANEWGLFA
                     AGSLVVILSFVWDFWKYLRAISFMDRSDVLSGSQYLDGILSDYIPSTFNWPLFLAGLL
                     VLAYGTLVLIRRFKSEKPVSANL"
     gene            complement(34065..35345)
                     /locus_tag="PKM27_05135"
     CDS             complement(34065..35345)
                     /locus_tag="PKM27_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06680.1"
                     /translation="MESKTGNPGEILPLEDEILANLDYPRQLEQLYQINKKAFKKAFN
                     RLYPDIREKPMVPYWHERLNFENGDLSWGNSKEWTFILVAAFLAGLVAKIPDFSWLDQ
                     DFFYPRNLGFIVFPFLSAYFVWKQKLAPKTFLPLLAVILGSAGYINLLPDNPESDTLI
                     LACIHLPLLLWAVLGISFVGGDRNNPYRRLDFLRYNGDLLVMTTIIGIAGALLTGVTI
                     GLFELIDLKIEELYFHYFGIWGLAAAPILGNYLIQINPQLVKNVSPVIAQVFTPLVLA
                     MLTIYLVAVIYTGKDPFNDREFLLIFNALLIGVMAIILFSIAESSKNGGNGQRMMLFA
                     LSGVTILINGIALSAILFRISEWGITPNRLAVLGGNLLMLINLLLVSYRLLKTIRNKT
                     EAETVEKSIASYLPVYVLWALVVTFIFPVLFGFK"
     gene            complement(35398..35697)
                     /locus_tag="PKM27_05140"
     CDS             complement(35398..35697)
                     /locus_tag="PKM27_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNR06681.1"
                     /translation="MKLLINGFHKAFESRIRLGIMSALAINDKFDFNALKEFLEVTDG
                     NLASHIKALEKEEFVGVEKSFIGKKPNTKYFMTPKGKKAFENHLKALENLIKKSG"
     gene            complement(35694..36320)
                     /locus_tag="PKM27_05145"
     CDS             complement(35694..36320)
                     /locus_tag="PKM27_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06682.1"
                     /translation="MKEEQDYLRDITEIRSMMERSSKFLSLSGWSGILAGIYALAGAY
                     IAYSFLGFNPADLEYRQVPGEILNFDLWKIVVLALIILILAVGTAIYFSQQKAHKRGE
                     RIWNATTRRLLAHMAVPLIAGGLLILILISKGWLGLMAPLTLLFYGLALYHISRFTYD
                     ELKTLGLIEMVLGLAGAYFIEYGLLFWALGFGLAHVLYGIFIHYRYER"
     gene            complement(36455..37435)
                     /locus_tag="PKM27_05150"
     CDS             complement(36455..37435)
                     /locus_tag="PKM27_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR06683.1"
                     /translation="MDPIHIVFMLYSKAHLLDLAGAAQVFYEANSFGTRKYQLHFVSL
                     KESISTEQGLRFAGLRPPEAVPLRKQDMIVVVGVDFKSFVRGEMDAEIEQTASWVIKN
                     YKKGVYLSSICSGALILAKMGLLNGTKCTIHWKCLDYARKKFPRVRFMDSQLYVFDKG
                     IFTSGGMTCGIDMALALVEKWSNPLVSAEIAQEMVINMRRAETQNQKNTFLDFKNHFN
                     PEVYKAQQLLVNNLQSSYTLTHLAADLHMSVRQISRLFKHHTGESIQSIRHKLRLERG
                     EHLLRNTELTLKEIAGQCGYQSPRQFSRIWKAYYGGAPRSQRVFKKSLSE"
     gene            37643..38869
                     /locus_tag="PKM27_05155"
     CDS             37643..38869
                     /locus_tag="PKM27_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNR06684.1"
                     /translation="MKKIILGCLAWILWNAAQAQFKNDNLVYKTVYLEDLCQSVKQLN
                     GGLLLDVRSPGEFSDTSAYHSLNIGRLKGAVNISSREIQKRLDEIRDYRDKPILLICS
                     HSQRSRVVSKMLVDSGFTNVTNVNGGMTELNRLRNGSIPCLNELYTSGNEFNLLSPAG
                     AFNLVRNTRDLYILDVRPDTAFQGKTASAMLRAHGRFKKAVNQPLISLKENTDKIPRD
                     KPILILDQFGDDAVQAARILADRGFKGIHVLFDGLAAWITETEKDLKERKKIWEKGSS
                     YGLINSQDLHRRFKGAYSGLILDIRPYADFNNQNKNQSFRNKGHIRGAINIPENELKS
                     RLQELTPYKNKEIIVYAFSGNPEAFAAAEILIKNGFKKVKVLTGGLFALRWEAANLIG
                     MKEMMNWVVNVPPENY"
     gene            38910..39827
                     /gene="mtgA"
                     /locus_tag="PKM27_05160"
     CDS             38910..39827
                     /gene="mtgA"
                     /locus_tag="PKM27_05160"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNR06685.1"
                     /translation="MQERTARFRKTLDFFWNISDKLVLTLILFQLVYFLILYFSELKE
                     RKAELIPFLTWFIVFLAATALLVGIYKIYFQKKDTIRRVVTAFYRIFIVAYLLQWFFV
                     LLYWFINPPLTFTQISSWIAGHGLKRDYVDYDAISPNLKLAVLASEDQLFPDHDGFDI
                     KAIKLALKYNKIRPKRSKGASTISQQTAKNCFLWQRRNFIRKGLEVYFTFMIETFWSK
                     KKILNRYLNVIETGKGLFGMEAAARYYFNKPAKALTRTESAMIAACLPNPRKYTVKPM
                     SAYVRSRVEYILVQMNNIEPDPDIQSLIR"
     gene            complement(39865..40494)
                     /locus_tag="PKM27_05165"
     CDS             complement(39865..40494)
                     /locus_tag="PKM27_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06686.1"
                     /translation="MKESDEKNNKALLVRLANQLLKEQQDLDHLAVQIALGKSKVIDL
                     FEEAKVQMKKRLQEFKKLLPPPAQLNDTWFKSLNERVDHLEEMLNKGKAKTKVLFQEQ
                     KKNIIQALDKLKNLLKQRPELIKFAGHLTGAMEKLKLQMDLFEKKMGAEKKKLTKEFK
                     AEMYDAAKKVNSIVSQVKSRQHDAGVKWEHFNDEIKISYAHLKKAIKSL"
     gene            complement(40603..43341)
                     /locus_tag="PKM27_05170"
     CDS             complement(40603..43341)
                     /locus_tag="PKM27_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR06687.1"
                     /translation="MSKSHKPVPGPAVPSSELSRFALMESPSMELDLIKTGLEKTHII
                     SIADEDQKLVYVNDLFCAISKYSREELIGQDYRMLSSGLHSPEFIKGLWDTIAGGSVW
                     NNHIRLKDKEGSYFWLDTTIFPFINKIGKPYRYFSICKDITRIKQEENTLNQFFQMSQ
                     DYLCIANAAGYLTKVSPQFINELGIPPELIHSQPFTAFATPRDAGPLALEQELEKRSK
                     GIPSRNFETKLQMRDGSFRLISWNASSDLETGTIFSIGRDITEIRKLNEEYRRLALVA
                     ESTDNVIVITDKEKRIQWVNRPFEKLTGYSLEEALGKDPGKLLQYDETDPETVRQIHE
                     ALEQKNSFYGDIKNRSKSGRPYWLRMNINPVFNENRELINFIAIESDVTEEKRAEAEL
                     IEAKQTAEKAVLAKDSFLANMSHEIRTPLNAILGFTDLLVQSNLNEVQSDYIHNIKNA
                     GDHLLLIINDILDLSKIESGNLVIEANPFSLKNAMTHLYKLLRVKAADKDLEFSLFMD
                     PDIPDMVIGDKGRLNQILMNLAGNAIKFTLSGEVIITVKKLEETKESVTLRFSIKDTG
                     IGIPEEKLSTIFDRFTQAEDSTSRRFGGTGLGLNISKQLVELQKGSLHVKSKPGLGSE
                     FYFILEYKKTADQQQESVKEKVVRLKSDRRLSILLCEDNVINQRLAKQVIENFGFDIT
                     VVNNGQECIDCLPKRKVDLILMDLQMPVMDGYQATHHIREVLKSDIPIIAMTAHSLVG
                     EQQKCFEIGMNAYVPKPFKQEDLFEKIQALLVNRPGADRSHDLPPAPDPADQKVVPAA
                     VDLSYLKELSNGNAAFEKEMIELFIQKTPQDIELLEKALQEPDYNTVKNIAHKLKSTF
                     ALFKLDESLIPLSQIETEAGNPPLSIRALEGFASIKQKLEEIMLGLQAILEQDYLS"
     gene            complement(43479..45839)
                     /locus_tag="PKM27_05175"
     CDS             complement(43479..45839)
                     /locus_tag="PKM27_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06688.1"
                     /translation="MILFLLPATGQERRFSITGRVNDENKEPIAYANVGLYRSADSTL
                     VSGGITDDDGIFSIESPPGSFFLKITFLSYVEKFIPGILLASRDLDLGSHTLVQASAL
                     LEEIIIEGERSTTELRLDKRVFHVGKDLTNISGSASDILNNVPSVTVDVEGNVSLRGS
                     QNVRILIDGKPSGLTGINIADALQQVQASLIESVEVITNPSSRFDAEGEVGIINIILK
                     KEHREGVNGSLMLNAGRPDHFGGTLGLNFRREKLNFFTSYGFNYRQSPGSGISSVRYT
                     LPDSVFAYTEETDRVRGGHSHNLIGGLDYYFNESSVLTGSFVYRPSSGLNTSLIRFRD
                     LDGNDRLLRTLSRREREEEPETNGEVSLSYRKDFKKQGQQLSGDFKWIENVEKEISSF
                     EQTDDRDGTVARQRSSNTENERNVFFQADYVQPFSETGKFETGLRGSLRVIENEFLVE
                     VENNFSEWDILSGFNNALEYSEKIFSGYAMAGKAWKRWSYQAGIRGELTGISVDLKRT
                     GEGNRQNYFNLFPSLFTSYKLSESHTFQLSYSYRISRPRFRHLLPFSNYSNNRSLSTG
                     NPNLRPEFTHSVEGSHLFNWSSGSLLSSIYYRGRRGVIEEITLVDSIGFSRQFPINLS
                     REAAFGFEFNLSWSPGPWFRSTSNYNFYRVSSEGVYENQTFAREALTWTNRTILQFTV
                     RKKWDIQGTFNYQAPQNTAQGRRLSLHSTDLALSRDLMKGNATLAISVRDLFNTRRFR
                     REITGVELNSYSEFQGRRRQLMLSLTYRLNQKKDKGEGDEPFEEDF"
     gene            complement(45970..47028)
                     /locus_tag="PKM27_05180"
     CDS             complement(45970..47028)
                     /locus_tag="PKM27_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06689.1"
                     /translation="MFRFLPLFVLQHPHFNKKTAWVVVVLFNLLLACTEEEIKSGPVR
                     AEIPLREVPGKAVKDPGLDTLLYHRLIQDLANGDSSGLWPAAAPLPLAGAVLPFKRIL
                     AFYGNFYSPKMGVLGALPRDKLIHQINSEMQRWNAADPSYPVIPAIHYIAVSAQKQAG
                     RDSKYRLRMPPGQIERALDLAKAINGLVFLDVQVGKGSLLDEIHYLEPWLLQTNVHLG
                     IDPEFSMKEDSPPGDRIGSVDAEEINTASQFLARLVRQYQLAPKILVVHRFNKRMLTR
                     YRHIQIRPEVQIVINMDGFGFPAKKRNSYYQAVYKEPVQFAGFKLFYKNDTLDGYALM
                     SPEQILQLKPRPVYIQYH"
     gene            complement(47158..47646)
                     /locus_tag="PKM27_05185"
     CDS             complement(47158..47646)
                     /locus_tag="PKM27_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06690.1"
                     /translation="MKKFLFLILLLIYQSLAGQELRLPNYTLNAGGHEMRDGNLVLRG
                     SVSQPLIQVVASGQVQNSQGFWHIHSSLKNQSTPYLNGQPDFRILTYPNPFDAGLHVT
                     LPEGQWPVRCFLYDLSGRIMGEWVLTAAKNTLALDHLAAGSYILYLRHDHWWYSCLTI
                     KG"
     gene            complement(47659..50349)
                     /locus_tag="PKM27_05190"
     CDS             complement(47659..50349)
                     /locus_tag="PKM27_05190"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNR06691.1"
                     /translation="MYQSIFVFALILSSSLLQAQVPKQFSYQGILADAAGKILPDKNY
                     TIQVLIYDQATGGNPLYSETQQVFLSNGVFHYQIGQTTPIPSSLLFDKPYYLAVRVDG
                     GEEMSPRSPLSSAPYAIRAIYADQVTSGWSLNGNKGLTGMHFLGTLDDRPLLFRLRDT
                     SAGYLGMDNVFWGYRAGEKNIGNENVGIGSFSLAQNQGVKNTGIGFGSLGINKNDMNT
                     AVGAYAMANSSQGKNNTALGTYSLKSNNGENNLAIGSRAMENSASGKNNIAVGGNALL
                     NSNGESNLAIGFAALQESITGKNNIAIGEGALYSNNGEMNISIGLASMQEGAQAKNNI
                     ALGNTALFNNNGENNIALGNRSMENSTLGKSNTAIGFLTLYENNGEDNTAIGFRSMEQ
                     NAEGARNVAVGAYSLFSNRGFNNTGLGYGAMEKSLSGVNNVAVGTAALWSNMGNGNTA
                     VGVNSLASNSSGKTNSALGIFSLNSNVSGEDNTAVGAGALALNITGSSNTAIGNLALS
                     QSSTSSNTALGASALELSIDGSKNVAVGARSLFQNNASYNTAVGTGTLEQNSTGFYNS
                     VLGFEAGGLNSTGSDNTAIGSQTLRSNVSGHKNTAIGGFSLNNNTASENTALGYSSLL
                     SNTTGSFNTSVGNHSNSSNTTGRWNTVMGHSALLTSATGNSNTAIGFEAMRNSNNSSF
                     NVAVGNLALTNNVGNGNVGIGNFAGFFNGLSTTHTNTTFIGENARSSNLSVQNSTAIG
                     SGAVVTASNQIILGNTAITQIRGNVGFSTFSDARFKTSVKEDIPGLAFVERLRPVSYT
                     LTHAGDPRRISGFLAQDVEKAANALDYAFSGLHLPKNDQDTYSLTYDNFVPSLVRAIQ
                     EQQAEIRDLKNQLEELKSRMKSMESLSQRLESLEAMIIKN"
     gene            50545..50979
                     /locus_tag="PKM27_05195"
     CDS             50545..50979
                     /locus_tag="PKM27_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06692.1"
                     /translation="MNTGISFSRSRYLAMFIFLVIMLATSIGKISAKDVNLGKISAAD
                     LKAQCEAEGGTFTITPDGENWICTKRCGSSICLVICDIEGGCSGHSPGKPADHRPVTH
                     TVSEVLNGKIKDGSGTIIPWLIPGLIILLVLGGYWVLNKRRS"
     gene            complement(51087..52130)
                     /locus_tag="PKM27_05200"
     CDS             complement(51087..52130)
                     /locus_tag="PKM27_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNR06693.1"
                     /translation="MNGIGNSPLLEVFLVLAISLLLGFLIKKTVIPFVLRIAGKTKWK
                     GDDIIAAAVSPWILFWSLLAALLYALPVLRGSLAFTARHESLFRSIIHTAFIFSITLV
                     VAAIATRLLYSLSSEQDREYASTSILGNTLRAIIYSIGLMLALRLFGIAITPLLAALG
                     VGGLAVALALQSTLSNLFAGLQLIASKKINIGDFVQLEGGQKGFIKDITWRNTDIETA
                     QNNIIIVPNSKMTDSIIENYFINSPEVLFNVSVGVSYDSDLEKVERVCLETGKKILEE
                     SAGGVKYFEPFVRFYQFGESSIDLRIFLRSKTFGDQFLITSDFIKTLHRRFREEGIVI
                     PFPVRTVLIKSQT"
     gene            complement(52189..53043)
                     /locus_tag="PKM27_05205"
     CDS             complement(52189..53043)
                     /locus_tag="PKM27_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNR06694.1"
                     /translation="MIRVGEFNMLKIIREASNGVYLDDGGEGLLLPKRVVPEGAKTGD
                     ELKVFIYHDKEGRPIATTLKPKGVVGDILRLRVTGTSPHGAFLDNGLMKDLFVPRNNQ
                     FGPMMPGHEYLVKIYIDRLSGRMVATEKLDPFLSNEALTVKVLDEADLIVYRKTPIGY
                     VVIINQRHTGVLHQNEIYRDLRIGDKMKGYIKNISAENTIDVVLGARGYQRVEEGGDQ
                     IIRLLQEHGGYLPYHDKSDPDEIYSFFGMSKKTYKMALGALYKQKKISLENAGIRLIT
                     TSPGTLST"
     gene            53176..53643
                     /locus_tag="PKM27_05210"
     CDS             53176..53643
                     /locus_tag="PKM27_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06695.1"
                     /translation="MKKTYLIIASMLFLLNTVTQAQSFTPWTWDTYGLAFKAPSNMIV
                     ETNNGEEFGAKLKNDLLHLYIFPWKDAELTTGSLNAEVEALAESLGYEGSEVVELELN
                     SFEGSYVIGKKDEVTSIILGLLDPESENNFYVLIVYGPGYEKRALEIANSFSK"
     gene            53720..54052
                     /locus_tag="PKM27_05215"
     CDS             53720..54052
                     /locus_tag="PKM27_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR06696.1"
                     /translation="MGAAKSDEFSVKDNRIASYAKALSHPARVAILKLLVKKNACFCG
                     SIVEELPLSQSTVSQHLKELKEAGLIKGDIEGSRVCYCIDAREWQQAKSLLGDLFSSY
                     EAPPSNCC"
     gene            54105..54941
                     /locus_tag="PKM27_05220"
     CDS             54105..54941
                     /locus_tag="PKM27_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNR06697.1"
                     /translation="MIQTDEQLKALVKQKYGEIALQDKETNQSSCCGSGPCSTGTYTI
                     MSDDYQELGGYKPDADLGLGCGLPTQHARIRKGDTVIDLGSGAGNDCFVARSETGEDG
                     KVIGIDFTPAMIEKARANALRAGYYNVEFRLGDIENMPLSSNRADVVISNCVLNLVPN
                     KDAVFKEIFRVLKPGGHFSISDVVLVGELPEALKRDAELYAGCVSGAIQKDAYLELIK
                     SNGFGNITVQKEKTIVLPDEILSAYLNEMEIRDFKEAKQGIFSVTVYAEKPKSCCGSD
                     CC"
     gene            55318..55710
                     /locus_tag="PKM27_05225"
     CDS             55318..55710
                     /locus_tag="PKM27_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06698.1"
                     /translation="MNKQAPLIWASVLLLFAVLTLFMSSSVLFDMFGIREREGNYVPF
                     VVWANFFASLLYMASVYGLVHRQKWTVRVLGMALILLVVTFTAFLFHIRSGGLYETKT
                     LGALVFRTGVTGLFTFIAWKTSQQPKRT"
     gene            55714..56274
                     /locus_tag="PKM27_05230"
     CDS             55714..56274
                     /locus_tag="PKM27_05230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HNR06699.1"
                     /translation="MKSEKEKMLAGEKYDPTDKVLANERTRTRLLLLELNNSREDDPE
                     NRMRILKDLIPNSGPNLWLQPPFYCDYGSNMLIGERVFFNFNCVVLDVMQVRIGSRTK
                     FGPNVQIYTATHPIDHEERASGLEFAKSIYIGEDVWVGGSAVICPGVTIGDRTIIGAG
                     SVVTRDIPSDVFAAGNPCRVVKQLAS"
BASE COUNT        14796 a        13101 c        13816 g        14618 t
ORIGIN      
        1 gacacgtttc ccaggaagag accgaaaaag atacatagga gtagtcagta gtcagggatc
       61 agtaatcagt agtcagaggt cagggtcctc agaagggcaa ggtggatttt tttaatttgc
      121 accacggtat gagccaggaa aaaacagcta ttaaaaccac ctatttcagc atagccggga
      181 atgccttgct ggccataata aaatggctca ccgggtattt cggtaattct tatgccatga
      241 ttgccgatgc cattgaatcg accacggata tttttgcctc cgtcctggtt ttgatgggca
      301 taaaatacgc cagcaaaccg gcagacgaaa accatcctta cggacatggg agggcggaaa
      361 ccctggtgac ttttatcgtg gtcgggtttc tcatggtatc cgctaccgtg atcgcctttg
      421 aaagcgtccg gcatatccgc ctgccgcatg aagcgcccga atcctatacc ctttgggtcc
      481 ttgcagccat catcctgtgg aaagaactgt cctatcaatg ggtgatccgg aaaagccggg
      541 aaaccaacag ttcatccctt aaggcggatg cctggcacca tcgcagcgat gcagtcactt
      601 ccgttgcggc tttcatcggg atatccattg ccttgatagg cggagaaggg tatgaagcgg
      661 ccgatgactg ggcggctttg tttgcttccg cttttatcat atacaatgcc tggctgattt
      721 tccggcccgc cctgggtgaa atcatggacg aacatcaata cgatgatttg atcgcccgga
      781 tccgggacgt ggccgccacc gttgaaggag ttaaggggac agaaaagtgt tttatccgca
      841 aggcaggcat gaaatacctg gtagacctcc atgcaacggt ggaagcggaa attccggtaa
      901 gacagggaca tgaaattgcc caccggctga ccgcggtgct caaaaaagaa atacccctga
      961 tcgggtatgt tctgattcac attgaaccag aataggattg aggattgagg gttgagggtt
     1021 gaagggtcaa acttttaagg gatcgggatg gatccagggt ttacggtagg gccgctggag
     1081 cagtttggtg gcttccgcat catcctttac gattcgtttg acgggatcat atctcattgc
     1141 ccggccggtt tccatggcat gattggccag gatacaacta gcggtggaga tatggccttc
     1201 ttcgatatcg gctacggggc gtgttttatt ttcaatggct gctataaaat ccagcatgtg
     1261 caggcgggtg gccggggccg cattgagttc tatccgctct tcggtcaggt cttcggggta
     1321 tttttctttt tcatagacta cgtccaggtg tattttctgg cctttgggat cgaccgggat
     1381 aaagtcgcag cgcatggtgc ttgcgatgag catgcccttt tctccatata aggtgaagga
     1441 ccagggataa tcgggattgt ccgaattgcc ccagctccgc tgctgccaga cacaatggag
     1501 ttcgtcgtat tcaaaaatgg cggtttgggt atcggcaata ttggatttgc ctcctttctg
     1561 aacaaagatg ccgcccgcag acgtgatttt tttgggccag cccagtttca gcatccagcg
     1621 taccgtatca aacatatgga cgcacatgtc gcccatgatc ccattgccgt attccataaa
     1681 agtcctccac caccgaagat ggggcaggcc gtcataaggc ctcagcgggg ccggtccggt
     1741 ccacatttcg taatccagga aggaaggcac gggttccacc ggtggatttc cattgttgcg
     1801 catatggata taacagcaca tttccacatg ggagatcttg cccagaaggc ctgcatccac
     1861 gatgttcttt ttggcgtcga tcaggtgcgg ggtgcttttg cgctgggtgc cgacctggac
     1921 tactttgttg tattttctgg cggcggcaac catggcttcc ccttcgatca catccacgct
     1981 gatgggtttt tgcacgtaga catgggcgcc cgcttttact gcgtcaatgg tttgcagggc
     2041 atgccagtgg tcgggggtgc cgatcaacac caggtcgagt tcgttttccg ccagcaactt
     2101 ccggtagtcg ccataaagct tgggcgtctt tcccgatttt tgcctttggg ataccagttt
     2161 tgcggctcca tccagcatgt ttttatccac gtcgcaaagg gctacgacct ccacggggct
     2221 gacctggatc aagcggaata aatcgctttt accataccag ccggcgccaa tcagggcgac
     2281 ccggtaattt ttagccgggt aaaacaagtc caggttcggc aaaccgaagg cagaaacggc
     2341 cagggaggcg gtagagcttt gaagaaagcg gcggcggttg atgttaaaag gttccatgtt
     2401 ataaagatag ggaaaggtgg gtggtggaag atgggcggtg ggtggaaaaa attgcagtaa
     2461 aaaataacat tccaggtttc gggtttcaga attaaccctt tcagggtaca aatgaggaaa
     2521 tatctatcca tctgtcatcg tctgaaattt cggaccacca cccaccaatt ttccatagga
     2581 tattgacaaa acaaaaactt atcgtatatt tgcgatatac atttgatatg gcttatacca
     2641 aaaaagaaga attcggagca agggaacagg ccctggcggc ctttgccaag gccctctccc
     2701 acccggcccg gataagcatc ctgggcatat tatccaaaaa ggaagaatgc atttgcggag
     2761 agattgtgga tgacctcccc ctggcccaaa gcaccgtaag ccagcacctg aaggaactca
     2821 aagaagcggg actcatcctt ggcgaaacgg ttgggccaag gagttgttat tgtatcaact
     2881 ggaaagcgtt tgagcggttt tattcggagt ttagtcagtt gtttcacaaa ctaaaagagc
     2941 agcagggaag gtgttgttga ataggattta taggatttat aggatttata ggatttatta
     3001 ggatgaatag gatgaatagg atgaatagga tgaataggat gaataggatg ataattacat
     3061 tttttgctat tgggtttaat tatcttgctg tgggccagat tcgttatccc caagggtggg
     3121 gacattatcc ggttcttgca aagtattcaa accccccccg gccaggtcag gcaggcacgg
     3181 actcgatgac gtttaagctc attcccggag ataccaaggt catccacttg gaacgcgctg
     3241 gagaagacca ttcaaaaact gttggaaacg ggtattccca gctaactgcc aatggggcca
     3301 ttcgcaaacc gttttattta tcacatgatg aaccctttag tattgaagat ccgccagcca
     3361 acagcagcgc ccagaccagg gccgaattgg acttcctgct tgagttacag agccggcgca
     3421 cggaagaaga catccgggct tccaaatact tcgcttccgt tttttaccgc acttccaccc
     3481 gacccgggga tgcggattat gaacgcatgc agcgaaatct gtttcacatc ggccgttctg
     3541 ttgccccctg gtttaatgcg gattccctgc cggccaccgc agaactggtc aggcgggttt
     3601 gggccgatgc ctcctatatc ctttggaagt gcaaaaacca ttttgtccgc atccggcctt
     3661 ataaactcga tccggccttg aagaacctgg aggaaaccaa ctgggcagcc tatcccagcg
     3721 gccatgccac caattcctat accaatgctt acctgttttc tgaattaatg ccggagtttt
     3781 catccttttt tatcaaggat gcttatgata tggcccattc ccgggagatc ctcggggtac
     3841 attaccccag cgattcagag agcggccgct tccttggcta cgagctcatt cagaagctga
     3901 aaaccaatcc tgtattccgg gctgattttg aaaaagcaaa gctcgaaatt cagtccatga
     3961 aaaaacggaa gggatatgga agttaaaatc tgatgcctga tcgctgaatt ctgaaatcta
     4021 ttttttcact taaatcaata accatgaaaa gcagttcaaa agtgcaggac cccggcggtt
     4081 gctgcggtgg attatgcgat tgctaatccc atgaatggtg gatggtggtt ggtggatggt
     4141 gattggagcc attccgctaa ccgcctttca tcaaattgtt catggtggga tttccaaacc
     4201 tttttaaact tttaaaggat aatttgttga cggtatccct aaaaaccgga taaatacgat
     4261 caaatcgtcc ggctggattt gccttcgctt ggaatacaac acgggatata tctaataacg
     4321 gtatagatcg gcgaagaaac agtcagtaaa aagttgattg ttaaaaaagc tttccagtga
     4381 gaaaccccga atcccgaacc ttgttaatat tgggctaaaa tcatggttac cgaattttaa
     4441 gttaaatcat taggactttg tttacagtta aattccatta aaaaggcaag ggggcatagt
     4501 tcttgttgtt ttcgccatat aattatttac caggcacaaa agaaagcacc atgaaagtat
     4561 atcccaatcc ggtttttaac atcctgaccc ttcattggga gccctctgaa gcccattatg
     4621 cggacctcat catttcaaac ctggatggga aggtggtgtt aaaacaaaaa gtggaatccg
     4681 ttacgacacc ggaccaccgg ctggatttga aaatattagg cttatctccg ggtatgtatt
     4741 tgatatcctt gatcaaccgg gacagcattg taagtaaaag ggtgatttta acgggttgaa
     4801 atcaagaaaa ccagttagcg ttatcacatc gaccccttta cgtttttcaa tcagcagtta
     4861 agaaggattt ttcatcatgt tcgacccctt cagggtcaat gcctctcatt ccatagcccg
     4921 agggctgcgc cccgggctat ggatgttgaa tcccttcgga attcataggg cactaaaagg
     4981 tgaaaatgac cctggtttag ttaaatcctt tcggaattcc tgccccgggc tatgaaggtt
     5041 aaatcccttc gggattacgg ggaaccaaat ggtgatcccg gtttgtatag cttatcttcc
     5101 tacaattcaa tatgattact ttttattgta gctcaatcct gactaagaat attgtaggtt
     5161 aattattttc ggaattccgg ttggcgaatg acaacaaacg gcatttcttt aaatcccgaa
     5221 gggatttaat cccttagcgc cgaatgcaat tcggtgcata aaaagacacc ttcctgcaac
     5281 cccgcagggg ttgaatcttt aacccaaata tttttcatcg tattccaccc cggcgtcatt
     5341 aagaaattca ataaattcct ccatagagga ttttttttga tgatgtcttt cctgattatt
     5401 tacataggcg atcaaggcat ctttttcacg ataggagcag gtgaacgccc catacccgtc
     5461 ctgccatccg ccaaaggaag ggaataattt gttttctttt ataaattttg aagaggccag
     5521 tttgatgtct ttgatcagat cagacaggga tgtggaagga tgaagatcca tcaacatatg
     5581 caggtggtct tccactccat tgatccggta catatggcat tttttgtttt taagaattgc
     5641 ggtaatgtac ctaaacaaag cagggcggcc atcggcatgg agggtccgct ccctgtattt
     5701 tgtactgaaa acaatatgat aaaagatctg ggtgttggta ctcataaggg tgattgttgg
     5761 tgtgtgtgtt ggtcgcggcc tttgccggaa ctttaaaaat aaatattttg aatgaattat
     5821 acgacccctt cagggtcgat atttctatgg atattgaatc ccttcgggat tcatagggca
     5881 ctaaaaggtg aacatgaccc tggatgttta gttgaatcct ttcgggaatt ctgccccggg
     5941 cgtaatttta tcccccaatg cacaggttcc ttctcttctg ttttatcttt ccggtacatc
     6001 tcccttgttc cattgcgcaa aaatccctgg tcctccattc ccccgatgac cacctgcgtt
     6061 ttgtgatacg cgtctcggat ggaatgcttc aatatgaaat agactacaaa gggaaaccca
     6121 tcctgcaaaa aagtaacctt ggcatcctgc tgggcaatgg taatgatctg agtcaaaatt
     6181 taaaaacacg gaaaataaaa acgcgggaag gccttgacga atatgagctc atcgtcggca
     6241 aaacccatcg ggtccgtgaa ccttaccggg aaggaatcat aagcatcagg agccggtcca
     6301 ggcccaaatg gaaattcgac cttatttcgc gcgcttacaa tggaggaatc gctttcaggt
     6361 atgtgtttcc ggaccaaaaa tcaggggata ctctttttct ggccggggaa agctcctgct
     6421 tcaactattt tggagatccc cggtccatgg ttttgttgcg tcaaaatttc attacctccc
     6481 acgaaggcct ttatagccat gaatcactct cccgaataca ggaggatacc ctgatcgacc
     6541 tccctgcgct ttttatcacc ccggacagca tctataccgc catcacggaa gcggcacttt
     6601 cagattatgc aggtatgtat ctgaaaaaac ggaaggggat attaacaagc gccctgtccc
     6661 ccttgcccaa taaagcggaa atcaaagtga aatccccttt gccccatcaa agcccctggc
     6721 gggtcatcct ggtcagtgac cggatggggg acctgattga atccaatatc ctgaccaatc
     6781 tttctccgcc aaacaaaatc aaagacccct cctggatcac tccggggaaa accacttttc
     6841 cctggtggaa tggcaatatc ctccccgatt ccatcaaggg gggcaataat tttgaaacca
     6901 ataaatatta tatcgatttc tgcgcccgca acggcatcga ataccattcg atcgtggagt
     6961 atgggggcca tcagtggtat gtggatgacg gggaggttta tcagcccggg ccgaacgctg
     7021 atgtgaccag gcccgtaccc ggattggata tgaaaagggt atgcgattat gcacatgccc
     7081 agggtgtaaa accccgggtt tgggttcatt gggccgccct gtatccgaaa atagacacgg
     7141 ccttcgccct ctatgagtcc tgggggctca ccggcatgat gatcgatttt atggaccggg
     7201 atgaccagga aatggtccgc atccaggaag aaatgctgga aaaagcggcc cggcataaac
     7261 ttcatgttca gtttcacggg gcttataaac ccacgggcat gtcgcgtacc tggccccatg
     7321 aactcacgcg ggagggaacc ctcaattatg aggtgcataa atgggactcc accgtcacgc
     7381 cggatcatga tctcgacttg gtttttacac gaatgctggc cggacccgcg gattgtcacc
     7441 tcggtggttt cagggctgta cctcgggact cctttaaaat tcaatatata aaacctttcg
     7501 tgcaaggcac gcggtgccat ttgttgggga tgtacgtcgt gatggaaagt tatttgcaga
     7561 tcctcgccga ttatcccgat gcctatgaag gtgagcctgg tttcgacttt ttaagagaag
     7621 tacctaccag ttgggatgaa acccgggtca tcgccgcagc gccccgtcaa tatgtggtca
     7681 tcgccagaag gaagagggat ttttggtata tcggtgcaat caacaaccgg gaagaccggg
     7741 agatatccat tccgctgagt tttcttccgg ccggcaatca taccctggat atttataccg
     7801 acgccgggga tgtggaatac ttaccaaatc acctgcatcg tgaaataaag aaggggtccc
     7861 gtacggatat attgaaaatc cggatggggc cggggggagg aatggcgggg aaagcaagta
     7921 ttgaccaata aaaccttcaa agggctgcga ggtcaagtgt tcttaattct tcagcccgga
     7981 gatgaatggt tttatttaat tttttattgt taactctcaa aggtgaggtt tatcaacatc
     8041 catagcccag gggttgcgcc caagactatg gtaagaaaaa tatcaaaact aaaggggtca
     8101 aacaaacccg catgcaggtc ttaacgcgtc gatggcacct gctgagccgg aggaggcgcc
     8161 gatttcacct catacacaaa ataccgggta tccccctgcc aggggaattt gaaatacctt
     8221 tctttataat acagttccag gcgctcgccg gtggcggacg cctgcatgac cttgtttgca
     8281 atatctttat tcagcaccga aaaattccac acattcggcg taagggtgcc ctgggtattg
     8341 gtggaaggca tcagcaggag gttgagttct ccttcccagg tcttaaagat cacccctttc
     8401 ttggtcagtt tgatcacctg gcccgcccgc atgccgtcgc tgtagggtac aagggtaaaa
     8461 aaagcccccg ccgccacaag cagcaacagg aggaccacca gggtccggat catgtatttt
     8521 ttgatctttt tcatcatggt tttcaactat acttgtgtta acattcggcg ggtcaaaatt
     8581 agttcatttt caatgtataa cagacatagc ctggacgata ccatcgccgc cctgtccacg
     8641 gcgccgggcc aggcggctat tgccgtactc cgggtctccg gggcggaggc catccccatc
     8701 gtaaacgaag gctttaaagg caaaaacctg gaaaagacag aaagccatac ggcccacctg
     8761 ggcttcttga tggacgcgga aggaggggtg atcgaccagg tcgtggtgac cctgtttcgc
     8821 ggcccccatt cgtataccgg ggaggatacg gtggaaatca gttgtcatgg gtccacctgg
     8881 atacagcagg cgattttaaa ccggctgatc caacttgggg ccagggctgc cggccccggg
     8941 gaattcactt tccgcgcatt tatccatggc cgcatggata tcagccaggc cgagggggtc
     9001 gccgacctca ttgcggcaga atccagcgcc gaagcccggt tggccctgtc ccacctgagg
     9061 ggcggaattt caaaggaaat cgcaggactc cggtcccggt tgatcgagtt cgccagcctg
     9121 ctggaactgg agttggattt cgccgaagag caggtggagt ttgccaaccg aagtcaactg
     9181 aaaaccttgt tggaagaagc catcctcaaa gtgcaggggc tggtggattc cttccgcctg
     9241 ggcaatgcca tcaaacaggg gatcaacacc gtgatcgcgg gacgccccaa tgcagggaaa
     9301 agcaccctgc tcaacgccct gctccaggaa gaaagggcca ttgtatcgga tatccccggt
     9361 accacccggg ataccattga agaagtcctg catatcgaag gcatcccctt tcgtcttatc
     9421 gatacggcgg gtatccgcga ggcccaggac cagatcgagg ccatcggcgt acagcgcacc
     9481 catgataaaa tacgtcaatc ctctattcta ctctacgtat ttgatgtcac cgggcttaaa
     9541 ccggctgagg tgcaatctga tatcgcggtt ttaaaacatc ccgacctgga gattatcctc
     9601 atccccaata aaatggacct caatccctat gcgcatccgg aagattttgt catggaggga
     9661 atcacccctt ccgacatcat ccccacctcg gcccgcaaca atatgaacat cccctacctc
     9721 aaggaaaggc ttttgcaatc cgtcacggga aatcatgacc ttttatccca aaacatcctg
     9781 tccaacagca ggcacctgga ttgccttcag agggtcaaag aacacctgag aagggccctg
     9841 gaacaattaa attcaggcgg agggcatgag ttgatcgcga tggatatccg gcaggctttg
     9901 cattacctcg gagagatcac gggggaggtg acgacggagg atttattgga gagtatattc
     9961 acccgatttt gtatcggtaa ataatctgaa gaggtcagaa aacagttgat cgcagcccct
    10021 ttattcaaat attatagaac gggaaagtat gttaactcaa attgtgatga cagatctgta
    10081 aaaccattct aaatcagctt tgtttaccgg tcattagcag attcaggatg aggaaacaaa
    10141 tatttttaaa aatgaaatat tatttctatt tttgaaactt attctcattc gtggtagtta
    10201 tttctaaaac caagttaacg gactttggac gcaggcatgc agaagcggtt gaggctgtaa
    10261 atgactggtg ggaaaaaacc aaagaagcag actgggcttc tcttgctgac attaaaagga
    10321 cctttaattc cgttgattat ataggcaatg accggtacat gttcaatatc aaaggaaaga
    10381 agtacagatt ggttgccatg attttttttg atatcaggac actgtatata cgatttattg
    10441 atacgcacga tgaatacaat aaaaaggatt gtacaaccat atagttatga taaataagat
    10501 cagatcggaa aaagaatata aagcggtaat gaaaaccatt gagacgctat tggagaaggc
    10561 gaccaactcg ggcggttttc acctgctgcc caaagaagaa tctgccatgc tggcacagtt
    10621 ttctaaaatt gctgaggagt acgaagacca tgcgatgcag ttgatgccga taaaacccaa
    10681 aacaatacag gaagcggttg aattcaaaat ggcggaacaa aagctgaccc aggctaaact
    10741 tgcaaagaaa ttaggcattg gagcgcccaa actttctcaa attttgaacg gaaagagaga
    10801 acccgatgtc gtgttcttaa aggcggtgta tcaaaaattg aaaatcgatg ccggattcct
    10861 gctgacacat gtttaaacta ggagaaggca aacgtaaaca tcattgaaca atgttccctg
    10921 aagataaaac cgcaacccaa cgcgcatagc agatttatta cacgaaagga atagagtgaa
    10981 gcaacatcat ccacacctcc gccagcaaca atatgaatat cgtctatctc cggaagcact
    11041 tccttggaat ccattggaaa tgaaaatgcc aggctatccg aaaatatcct gactaataac
    11101 cgccaagcgg attgtttgaa aaagtgaaag aacacctggg aagggctgga acaattaaat
    11161 tccggcggag ggcatgagtt gatcgcgatg gatattctgc aggctttgca ttacctcgga
    11221 gagatcacgg gggaggtgac gacggaggat ttattggaga gtatattcac ccgattttgt
    11281 atcggaaatt gatgaatatg accttaaaaa agggatcagc aactcaatcc actcagtttt
    11341 ttatactgct aaacaccaaa tcttccaaaa aagcaacaat cttcgattca tctttatctt
    11401 ttccaaaatc ggtaagcgaa ggaaggttgt gcataaaaaa gttttgaaaa tgcgccactt
    11461 caatcaaagt gcttgcaagc gatttgggat atttatactt agggttgtaa tctaaaatga
    11521 tttctccaat ttttgcgcaa aggtctttgt aaggtttaaa caattgatct ttgttgtctt
    11581 cagtcacatg tttggttaaa taggctttgg tgccctcggc aatgatgatt tgatgaagga
    11641 tatcctcatt gaaataggtg gttttaatat catcctccac aaccgcagtc aataatttaa
    11701 tgactctttt gagccttacg gcagggtctt taatattcat ggtgtttacg gaaactcggt
    11761 attccagcca gctccaatac caggccgcga tatagatcag caagcggtgt ttgttttcaa
    11821 aatatctgta aatactggcc tcggtagtgc caatatctgc agccagtttt ttaaaagtga
    11881 aggattcaaa tccattttta tggattaact gtatgctgtg ctgaatgatt tttttcccca
    11941 gggcagatag ttccgggttt ctgagaaaca gtttttcatt catttttatc tgcagcgcta
    12001 tttccatggg ttcgggtttt aaggtaatgg tttacaaaca acgaaaaatt ccaaggtcga
    12061 caaatccaag cgtcaaaatt aaggaaagtt taacaatcta aaaagatagt aaagctatca
    12121 agtatgataa atacactatc tttgtgttgt taaactaaaa aaaccgacaa tggaaataaa
    12181 aaacattttt aaagatttta aaaacgacct cccggcgagt atcgtcgttt tttttgtggc
    12241 cgttccgctt tgcctgggta tagctctggc ctcgggagcg ccactgtttg cgggcatcat
    12301 tgccggcatt gtgggcggta ttgtagtggg tatggccagc ggctctcctc tgggggttag
    12361 cggaccagca gccgggttgg ccgtgattgt gttgtcctct attgcagcca tcggttcctg
    12421 gcccgccttt ttattggccg tggtgctggc cggaatccta caactcatca tgggatttgc
    12481 caaagcgggt tttatcgctt acttctttcc aaattcagtg attaaaggaa tgcttaccgg
    12541 tattgggctt ctgatcatat taaaacaaat acctcatgcc ctggggtatg ataaggatcc
    12601 cgaaggagac ctgtcctact ttcaaacgga cggcgggacc accatttcct ccatttcaaa
    12661 cgcaataaac gccctttctc caggggccat actgatttca accattgcaa tcgccatact
    12721 gatattatgg gatacggtat taatgaaaaa acataaactg tttcaggtaa tacaaggtcc
    12781 gattgtagtc gtggtccttg gcattgccat gaattacctg taccaaacag gcgccttaaa
    12841 tttcagtttg gatgcggacc aggtggtaaa acttcccgtt gcaaatacat taagggagtt
    12901 tttcagtttt tttacgttcc cggatttttc ggccattact aattttaaag tttgggaagt
    12961 ggcggtcgtc ctggctattg tggcaagttt ggaaacattg ctttgtgtgg aagccacaga
    13021 caaacttgac ccatataaaa gagttacgcc aaccaaccgg gaattaaaag ctcagggatt
    13081 gggaaatgtc atttcgggca tgattggcgg cttgcccata acccaggtca ttgtgcgcag
    13141 tacggccaat ataacctttg gcggtaaaac aaaaatgtca gccatccttc atggtttatt
    13201 cctgctcatc agcgccatta ccgttgcgga atggctcaat atgattccgc tggcaagttt
    13261 ggcagccata ttgattatcg tgggttataa gttggccaaa cccgccctgt ttaagcaaat
    13321 gtataaattg ggatgggagc aatttgttcc ttttgtggcc acggtggtgg gcatattggc
    13381 caccgattta ttaaaaggaa ttaccatcgg tatcttgttt gggatcttct atacgttgcg
    13441 gcacagctat cgaaattcgc atcacctgaa agacgtagtc actacgcggg aaggccagca
    13501 ggttcaccac atagaattag cccaggaagt gtcttttttc aacaaagcaa gcgtaatgcg
    13561 gaccttggaa gccatcccgg cccagtcgaa agtgattatt gatttttcca aatctaaatc
    13621 cgtggcccat gatgtgattg agatcataca gaattacaga atcaatgcca aggctaaaaa
    13681 cataaccgtt gaaacggtca attttattga accggcttag aataataaaa tattattaaa
    13741 tcaaataaac aatatataaa atgaaaacat tgactaaaga aatgcaggcg gccataactc
    13801 cttccacggc gcttaattta ctaaaagaag gaaacaaaag atttgtcagt aaccttaaaa
    13861 taaaccgaaa cctgttgcaa caggccaatg agacctccga tggacaacat ccttttgccg
    13921 taatacttag ttgcatcgac agcaggactt cggcggaatt gatttttgac cagggtctgg
    13981 gtgatgtgtt cagcgttcgg attgcaggca atatcatcaa tgaagatatc ctgggcagta
    14041 tggagtttgg ttgcaaggta gcggggtcca agattatcgt ggtcctggga cataccaaat
    14101 gcggcgcaat aaaaggagcc tgcgaccatg ttgaaatggg taaccttacc gccctcttaa
    14161 gtaaaataca gccggcggtt tacgatgaaa aatcagtgtc cgaaaaccga aattcaggca
    14221 accccgattt tgtggaaaaa gtggcaaccc ttaatgtaaa aagaacagtg acggccatta
    14281 tggaacgcag ccctatctta aaggagatga ttgaaagcgg cgcgatcggc attgtggggg
    14341 gtacccatga tatcacgaca ggcgaagtaa aattttatga ggatacctta atcattaaca
    14401 agaggggtga atgaaggtat tcagccattc ctattcgctc ttcagcacca ccgccggatt
    14461 actcctggcc gccttcatcg tctgcgaacc gatcagaacg atcgcaatca gcagtacggc
    14521 aagaaagccg gcgaacaatt cggggaactg cacggggctg tgataaggaa accggaggag
    14581 taccactttt tggaaaaaga ggtaggtcag gggcagggcg atgagggccg atacggataa
    14641 aaggctgaga aaaccgctgc ttaataagta gataaggtta ccctggcttg cgcccatcaa
    14701 tttgcggatg ccgatctcct tcagcctggt ttcggcagtg aacactacca ttccgaacaa
    14761 tcccatcgat gcgatagaaa tggcgagaaa tgacaggaat ccaatgattt tgattatagc
    14821 agcaaattcg ttgtatgcgt tttctatttc ttcgtcatag aattgagctt taaatggatg
    14881 aacacgatcg attttcttcc aggtggactc aatctttgcc agggtggcct gcatgtcagt
    14941 gctatgtatt ttggcattta tgattgcccg gtcttcggga gcccagggta taaaggctac
    15001 cggcccgatg acgttttcga ccgtgccgta gtgaaagtct ttcattacac cgacaatagt
    15061 catcttacgc ctgtgtacgt ggaaattact gaaagtgatt tcctgtccaa ttgccttctc
    15121 tggatcattg cccgcgatgt taaatcgttt taaaagttgc tcgttaacga tcacttcgcg
    15181 ggcggcttcg gccgtggtgg gtcgaacaat aaaatttccg ccagcaacaa gtttatactc
    15241 atgcagaggc aagtagtttt catcgacgtt attagtcaaa accagtgcag agtcctgcga
    15301 atttttgtag gtcatgtatc caccccaggc atttccaacg ctggtgacta tcaatgaccg
    15361 ggagagtccc gttacttccg gcatctcctt cagttccttg agaagcgcat cgggtttatt
    15421 cccctgcata tggatgttca ggatgttttc agtgttgaat cctaagtcaa acgaaagtat
    15481 gtttttatac tgcacgtagc cgacggcggt ggcggttata aagatgaggg tgagcgtgta
    15541 ttgaattacc accagggcgc gccggaggct caggtgtttg aaggctttcc ggggcggtcc
    15601 gttcctgaac gcatggctcg cgttgatttt tgaaaaatac agggcgggga caaaaccggc
    15661 aatcacgcct acggtgaccg aaaaaaagat aaaagccgct accatagccg gactgaggtc
    15721 gagctttacc atgttttgca tttccggaga caggcgaatc agttgcgggc gaagcaggaa
    15781 aaaaaggagg ctggaaacaa gaagggcggc cagggagatc atgatcgctt ccaccaggaa
    15841 ttggtgtctc acctgacttt tcccagctcc gatggctttg cgcaggccta cctccctgaa
    15901 gcggcgcagg gcacgggcta ccgaaagatt ggcatagttg aaacaggctg atccgatcac
    15961 cacgaaggcc aggccgacga ggatccaaag cagggccgga gatacatgag gtcccgaagg
    16021 acccatggat cggctgagtt gctctccgac tacgatctta tgcaatggaa gaagttcaag
    16081 ccggatccgg gtgtcccggt atccctcggg accctgaaca agattttctt ccctggcaat
    16141 ggcgtcgagt tgggactgga tggaaacccg atccgcattt tcctgcagga gtaggtatac
    16201 ctggtttgac cacatgtttg tccaggcctc aaaacgggga tcgtgttgat tcgctttttc
    16261 aaccgaagaa aacgatacca gggcttcgaa gctgatatgg gaaaagaaag gaacatcctt
    16321 catgaccccg gtcacctggt aagggaggga atcgaatttg atcgtcttac cgagtgcgga
    16381 ttggccgccg aataattttt tggcggcgga ctccgtgaga acgatgcagt aaggatcttt
    16441 cagcgccgtt tcaggattgc cctccaccat tggaaaagta aagatcctga acagcgaagg
    16501 ctccgcccag tagcccttga ggggaagaat acggtcgccc accacggcat cctgtgaaaa
    16561 atcgttttgc agaatgacca cttcttcgat gcccgtcatt ttttcccgga ttagttttcc
    16621 ggtcttgacg gaggttgttg caaattttcc gcccggtccc tggttgaagg tcgccacatt
    16681 ggtgatgcga cagatccttt ccccgttttt atggaaccgg tcaaacgaaa acaggtcaaa
    16741 cacaaaggcg atcagcaata gaccgacgga catgctgacg gcaaggccca tgatattgat
    16801 gaacgaaaag agtttgtggc gcattaaacc gcgccccgat gttttgatat aactttcaat
    16861 catgatccgg ctgataaaag ggttgaccca ttttggtttc tgaacggtat agggtctgaa
    16921 gaacttgagc gcgtcgatca tatagatcag tttcgcgcga cgggggccga cggaccgcag
    16981 atttcgctcg aagtattcat tgaggtcccc gatcagatct tccgcaagct cgggcttgca
    17041 gtaccactct ataaatttcc gtgcccagga gggaggctgg tgttgcggtt gttctttcat
    17101 ggtgttattt ccaggccagt gaggggatcc tgtcccacaa ctgatcgcgc atggctttgg
    17161 tccggaccag ggcttctttc cccgttaccg tgagttgata gaatcttttg cgttttccgc
    17221 cccgggactt ggtggcttca cccagctcac tcttcacaaa tcccttctct tcgaggcggt
    17281 ggagcaccga atgcaccacg ccaagtttca cggtacggcc gttgtgagtc gagagttcgt
    17341 cgcatatcgc tacgctatat gctttatcca tcagggcggc tatggtgagt aaaacaagtt
    17401 cttcgaattc tccgaggttt gtacccttca tctaagatat ttattacgca aatgtatgta
    17461 aaattattat attatacata caaaaggtga ttaaataatt tttgactgga gccattcagg
    17521 ccttaaatca caaacggaaa tattcgtccc tcagatatta gacaggccgt tgagtgcaaa
    17581 gaaatggacg gtgagccctt cgttgccttt actttttcta cggctggttt gtaaacaaat
    17641 tacacagata atgttaagta cttgaaccca aacatgcaat ggactttata ttacacgctt
    17701 tgggttgaag ttttgtcctg aggatatata ctgtaaaata aaacaggccc atccggcaca
    17761 gagactgctt tcccgcgatg gccctattta ccattccgct tccctaaagt aaaaccttaa
    17821 ttagacccgg tcactttagc cccctcaaaa ctaaagccca ttgcttccat tttcattttg
    17881 gtaaccgctt tctggtcatc catctggaat ttaagcagcg cctttccgtc ggcatcaaag
    17941 gtatgaggcg ttgcggtggg ggacagcggg cccgtctgcc cgttggcctc tattatgagg
    18001 gtaccatcct ttacagagat tgtaataaac tcaaacggca ggccggtcat ccggtatttg
    18061 ccggtgtatg cgttcaggtc accggtgggg gcaagggttt tgggttttac atccaccgtg
    18121 ccggttttaa accgggtatt cagcaagatc atcagggacc ccgctttttt taattccggg
    18181 tttgtgaaga cgagatagag aggatgaaac ccggtagtcg gctccaaatt gattaatacc
    18241 ggctttgggc tgaacccggc gccgtgatct ccgataaatt cagatttacc aatgagttta
    18301 cccgagggct gaccgatacg gacttcaata tatcccccgg cggcattcaa ttgggcttta
    18361 ggcgccatag ccatggcttc cagctgggtc agcccggtca gatcgatgtc ttttaatccg
    18421 acataacttc ccggtttagt gggaataaca aatttgttgc ctccaaaaga cattttgttg
    18481 acatcgctga attcttcgta atcgcttggg ttgattccgg gattgcgcaa aacaaaggat
    18541 tcttcagtaa gaattcccgg caaactgttt gctccatggt ctttgtaggc ggcccggaag
    18601 atataaacac cctggccttt atctccgtcg ggcagcttga tggtatactt tccttcaaca
    18661 ggcagtgatt taacttttgg tttatcatct gcaaggctca gaatatacct ggtcatctca
    18721 gcagcgtcct cagcggacaa ctgcggatgt ggagccatgg gcacctggcc ccagaccccc
    18781 tggcccccgg agatgatttt tttggcgagt acttgctgag ctccgggttt tcctttgttt
    18841 ttgagtgcaa tgtcccggta cgtgggcccg atcagttttt ttgtaacatc gtggcaggtt
    18901 ttacaatctc cggcggcaat gagtttcatg cctttggaaa aagccgcagt agcgtctgcc
    18961 gtccggtggc cctgggctat gatggttttg tcatggcctt ccgcgagata gtctatattg
    19021 accgatattt cctcgggatt tatttttcca ttggaggtgc ttccatcctc ctggtcatcc
    19081 accaccacct tgtactcgta tgttttatcc gggaaataaa aggatttatt gttttcccca
    19141 agatcaaatt tcaatacggg aggctcattt ccggcaatca gttctacgga ttgtgtggag
    19201 atcccgcccc ggttatcggc cgcagtcaag gtgactttat aaatcccggc ccggcttaaa
    19261 tcaagatcta cttcaggccc aacaatattt ttaataaacc cgagtttgga ggtaatcttc
    19321 caggcaaagc ttaaagtatc tccatccgca tccgtggatt ctttggaaga aagggtagtt
    19381 ttgaagggaa ccgaaccagc cagtttgccg gcgaccagcc tgatctccgg ctttctgttg
    19441 ccgccgttgt attcgatcct gaccagacgg gcatcatcgt tggccgaaaa ccatcctgag
    19501 ccatattcga gcaggtataa gtctccgtcg gccgaaaatt ccatatccat cggattgctg
    19561 aaccggtaac cgggcataaa cctttccatg gattgataat ggccttcctg gtccatggtc
    19621 acggccatga tccacccgcg catccactca tagataaaca gttttttatc gtaatacttt
    19681 ggaaacgccc ttttagccgt cttaaaatcc tctgaataaa aaacaggccc cgccatggca
    19741 ttccgccctc cggttcccac cagcggaaat tctttggatt ccgcataagg ataccagata
    19801 aaggcttttt gtgcaggggg aagcgcttcc aggccggtat tgtttgggga agtattgatg
    19861 ggttttgagg catcccacaa ttccagggac ttgctctctg caaaatcgta tttgtaataa
    19921 ggcttgttgt ccccgacaaa atggggccag ccaaaattac cggccaccct ggcctggccg
    19981 acttcatcat ggcccgcagg tcctcttgcc ggatctgcat cattggcatc cgggcccact
    20041 tccccccaat aaacaaatcc ggtttttttg tccacggaga tacggaaggg attgcgatgg
    20101 cccatggtat atatttcagg ccgggtctta tcggtgccgg cggcaaacaa attgccttcc
    20161 ggaatcgaat acgtcccatc cgccctgggt ttgatacgga tgatttttcc tctcaggtca
    20221 ttggtattgg cggaggattt ctgggcatcc cagggcatcc ggtcgggcct ttcatcgctg
    20281 ggactgtaac cgtttgaacc atggggattg gtattgtctc cggtcgaaag gtataaattg
    20341 ccatctttat cgaaagcgat cgacccgccg gtatgacaac attgctcgcg ctgggtaggg
    20401 accgccagca gcactttttt agacttcagg tccaggacat ccccaaccag ggtgtaccgg
    20461 gtcaatatat tctgtgactt accgggatcg gaataataca ggtagatcca gtggttgaca
    20521 ttaaaattgg ggtccttgtt caatcccagc aagccgtcct ctgcttctga agtttttcct
    20581 tcccggtctt tgtattgggt gctgaccggg atcgttgcga tggtcttaac ggatttggtc
    20641 cgggtattgt aaattttcac tgcgccatgc cgctcgataa acagaatacg cccgtcattc
    20701 agtacgctca gttccatggg ttcattgagt ttttccagaa ggatgacttt tgaaaaccgg
    20761 ttttcttcgg gtttagcttt agaataatcc aggatcacgg gtttttccga actcatggca
    20821 tattgaagcc cggcccagac gtgatttaaa aacaagggtt cggaaaaact ctcatcggta
    20881 tgtcccatgg ccgtatagaa ggccctgccg ccgtcaaaat catggtacca actcatcggg
    20941 tggtggtctc cgttttttcc cccgcggtat gatttttcat cgattttcac caaaacttta
    21001 atagtgggat cgatttgttt aaagctgtag aattcatcca ggcgttcaaa ttcatcgggc
    21061 atgccctgcg tcgcccagtt cttttcgacg acctggaatt ttcctttctg gacattgtcc
    21121 gggttggggt gatcgaggaa ataggcgccg gccaggcggc cataccagtt ccagtcgtat
    21181 tcgcagtccg tggccgcgtg aatgcccaga taaccgcctc cggcctggat ataccgctca
    21241 aattgagttt gctgttcatt gttgaggaca tcccccgtgg tattgagcca gatgacggca
    21301 ttgtattgtt tcaggacatt cgcagtaaat tgtcctgcat cttcggtaaa atccacgtca
    21361 aagccttttt cctccgacat ttgtttgaag gccagtttgc cggcatcgat ggaagtgtga
    21421 cggaagcctt ccgttttact gaataccagc acgcggagct tagttggatc gtcggagctg
    21481 aatgcaagta aaaagaacaa agccaggggg aaaacaaaca ggtatcggga ggagggaccg
    21541 gtaacgaaca taaaatcgga ttttgaggtt gagtaaaacc tgatcaaaga taagagacta
    21601 tggtataaat cttgaatcga cgggcaaacg agaaagcgcc atgtcccatg atggcaaaca
    21661 ttaataaaac agtgaaaaga atcacgcgca cctacttgtt tatcctgccc actgatacca
    21721 caagcgccca atccgtttct ccagcctgat ttttcccggg cggaacaaac ctactgatgc
    21781 cggtattgct aaaagccgac gtttgcaccg ggaggtaccg gccgacggta ggatcaaacc
    21841 agtatgcgaa gcccggttta ctcaggacct gcaaatccac gctgacttct cccgggtgtg
    21901 caggaggaaa ataggctaca agaaaggttc cgtccctggc tgccgcggca gctatataag
    21961 aagcggaggt atctatattt tccggatcct gaatgaaatt tttcattccg tttaaccccg
    22021 cgggtaccag tttccaccat tccaccgact taataaaccg gttgagcctg gccatgtctc
    22081 gggcgccccg ggtattcagg tgctgctgcc atagcgggtc aataaagggc cagacatatc
    22141 cattgcctga aatatatcct ccgatggtgc tcagccagcc ccaccattga aaacggcgca
    22201 cgggctgggt ggcgttcgga ttgtaattgt ttccatcggg tccttcttca tcgtaaggtt
    22261 cttcaagcag gaaggccggc atggggggat cgtgcgcata agccttccga cccaggtaag
    22321 ggaccggaac tttcaattcc caggtataaa caccgttgag ggtcatctca tggccgaagg
    22381 caatattgtc ggccgcgttt tccccgctgt gtgattcggc ggtgtattgg gtggactgtt
    22441 ggtctttcac acttttcagt ccgtcgataa gggcctgttc ggccatttgt tgttcgtcat
    22501 taaactttcc catgtcgccc agcagcatcc aaactatgtt ttttctcccc ttgtagcggt
    22561 ttgcgatcca ggcgccatag gccttggttt tttctttgcc gttggcgacc agttctttca
    22621 tccagccctg ctcttctccg ttgtatccca ggtaagccgg aaacataaag accagcagcc
    22681 ccttcctctc acagtaggca agaaaccggt cgacaaaaag ccagtatttt tcattgggtg
    22741 tggtaaagtc gggcgcttcc tggactaaaa tattgtattc aagtttaccg tcccaatccg
    22801 agccgttgag ccttttcaga aaaggcagtt cgccctctcc gttgaaaggc gcatgatttc
    22861 ccgtcggcca gtggatgatc accgcgatct aaaggcgttg tgaccgtgcg ccagggtgtt
    22921 ttcgacaaat ttccggtagg ctttttcagg ttgggaaaga atgcaccagg cggtgcggcc
    22981 aaggatcggg aagggtttgt ttttccggtc tacgagatgc ctatggtcat cgctgatgcg
    23041 gagaggaaaa gcggcctgcg catatacaca gtgaaaaaca gtgctaaaaa gtatagaagc
    23101 cgccaaagcc ttgatcataa cttttttgtt taatctgtga aaatatctat gaaaattaaa
    23161 attcaaaaat gaccttatac tgagccgaag ccaagcttcg tccaaatgag agataaaaat
    23221 tacccctaag ggtgaaaaat tcaatttaaa cagaaaccat ttttgggcca tgaaagccca
    23281 tttaccaatc cttgtaattt ttgcatttat cctgaatgcc tgcgaaaaag accaatccgg
    23341 cggagatacg gaaaagatta tttatggaaa gatccggctg accaccaaaa tagatgggga
    23401 tgaccgtgag tattttgtac atgttcccaa agcctatacg ggcaaacagg cggtccccat
    23461 ggtatttatg ctgcacggta ccagcgggga tggagaagaa ttttacgaca gatcgggctg
    23521 gaaggaagtg ggggaatcgc aaaatgtgat caccgtattt ccatcctccg ggagatattg
    23581 cattcatacc cagggtgaaa ctaacaagac caccaaatgg aataccgcgc ccgatgcaga
    23641 atggactttt tgcagcgggc aggcccctaa ggatgatatt aagtttctgc gcaccattat
    23701 cgatgaattg tcctcaaaat ataagataga cgccaaacgg atctacctag ccggtttttc
    23761 caatggaggc cagatggccg caaaatgttc catcgaactg agtgataaac tggctgccgt
    23821 tgtactaagc gccggaacgt tttatctgga caccatctat acgccaaaaa gaaaactgcc
    23881 ggtgctttac caggtgggca atgaagacta cggccccggc aatacaggcc cggccatccc
    23941 gatgaatcag ttctcttacc tgatcagcac cccgggattg acctttaaag acggcaggtt
    24001 caatatcatc gccacggatc acgtccgtaa ttttgcgctc aatccaaatt ttacaatcag
    24061 cggcgacacc accaaagcaa gtattgccac gtataagccg ctattgacga ccgaaacaca
    24121 cgaatttcag tatatattga tcaagggctt gaaacacgcc tatccaaacg gagacaacca
    24181 tccattggaa gcagccgaac tcaattggag ctggatgaaa caattcagct tgtaagcaca
    24241 ggggaaatgc ctgtttgcag tctaaattcc tggtaagagc aatttttcca gtccgaagcc
    24301 aagcttcgtc caaaccttcg aggattaacc aagttctaaa atgtccgcct gtactccaaa
    24361 gtggcaataa aactcctggt caatccgtcc ggcctttctt ttcttacaat aaaggggacc
    24421 ccggcgttca attgaaggga atggttgttg tttatttcat aatcaaaatg aacggtgccg
    24481 tttaaagtca ggccctggga accatcgatg gcccgctcca aaccctcccg gtctgtgtat
    24541 tggtctttgc ccaggtgaat gaccggcagc aaacccgggg taattttaaa ctggttgttt
    24601 aaaggaatgg aataagaaac ccgggccatt aggtctcccc tccgcctgaa ccggttagtg
    24661 gtttgaaact tttttaaggc cgagccggca ggataatctt ccgcggcgaa ttcgttttga
    24721 ttctgggcaa ggggctgttg gagggccaag gccaggtgaa atttattcca tacgcggctg
    24781 attccaagca ggaggtccag ggtacccagg ctggactgat aatccatggg caaaggcagg
    24841 ttctttttaa ttttacttgc gtcattcaat ggcaatttga atcccagggt gatttttcct
    24901 atggtgccgg cgggatagtc cgcactcaga aaaaggtctg aaaagccgaa agcggaaatc
    24961 ccattgccgc tttgggacag ggaggtgagc ctggcgctga aactcagttt tttattcaac
    25021 atccgctggt agtcaagatg ggtgccaaag atggtaatgg aatgatccgc ccgtccgaaa
    25081 ctcaatccga gtttaagctc atttttgaaa ccggacgtgc tgtccaaacc attgggttta
    25141 aaattgccca gggtgcaaaa tccggcatcg ctgcacccct ggccccgggc agtatatatg
    25201 agggcgctca gtccaatcat catcattatt aaacccaggc ttttattttg catagcttat
    25261 aggatttgtg ttattaattt actccaattc tgcccaatcg tgcaagatct tttttagcag
    25321 gggatgaagg acgatccggg cctggatctg aacaggggac ataaaatttt tatttcttgc
    25381 caatgcgata caaccttccc tgatctgtga tggtatacaa agccccatcc cggccctggg
    25441 taatgtcccg gaaacgctga cgctcgcccg aaaacaacct ctcttcgccg gtaaccctgt
    25501 tgttttcaat gaccagccgg cagatatgac caccgctaag cgtagcaata aataaattgt
    25561 ttttccactc gttgatttcc gcaccattat aaaacgtgat cccgctgggt gaaatgacgg
    25621 gatcccagta ataaaccggt tgttcgagcc cttccttttg ttgtatggca tcgccgatcc
    25681 tggacccgcc gtattcaatg ccgtaagtga tgacgggcca gccgtagttt ttgccggcca
    25741 cgatccggtt gagttcatcc ccgccccggg gcccgaattc ggtttcccac aaatccccgg
    25801 tcaccggatg aaaagcaatg ccctgcacat tgcggtggcc ataggaatac agttccggcc
    25861 gggcaccgga cctgccggca aagggattgc cgggggccgg cttgccatcc cgggtgaggc
    25921 ggatgatctt gcccaatccg gaattgatgt cctgggcctg ggggcgggtt gcccggtcgg
    25981 agcgctcacc ggtggtcaga aaaaggtggc cttcctttga aaagagcacc cggccgccat
    26041 agtgcagcgc tccgtcgtag gcgggagtgg ctcggtagat aaccgctacc tgctccaggc
    26101 ttttttcatc cgaagacaag atgccccggg cgattgaagt aacattccct tcgggcaagg
    26161 gttcggaaaa ggcccaataa accattcggt tgctcgaaaa agaaggatcg atgcaaaggc
    26221 ccaacaagcc cccctggccc cggctgttta ctgcaggaat gccgtcgagg ggatccccca
    26281 cggccccgga agcggaaaca atgcgcatcg taccgctttt ctcggtaatg ataaaccggc
    26341 catcgggcag ggaaacgatc ccccagggac ttcgaaggga tgaagtcagg agtttcaact
    26401 cataaggagt tttcgtcgtg accgcaccga tccgcgtctg gcctataaat gcaggtttat
    26461 aattggtatt ggcattttgg gtttccaccg atgcattggc ttttccgggc tgggcggtgg
    26521 actcacagga aacggccatg aggaaaacag ccaccaaaaa catacaattg gatttattca
    26581 tgatttaact cagatttgaa tcaggtataa ggttgaaacg ggccaccgtc gcggacgccc
    26641 cggaatggag ggccggatca tgcctgtttc gtttaatagt ttaacggatt ttcggtaaaa
    26701 ttaggcgtta tccctattaa aaacccgcaa ttcaggcctt aataatattt ttcaaaaagt
    26761 tcaggcataa gaccgcagta tgcggagaaa ctaaagagct atatccactc aaagtgactg
    26821 aactttgacc ggtacatacc gaaggctgaa agaccctgac tgacaaaaca agtttaaaat
    26881 atctatattg gtaaggtgac catggaaacc aatgatcgtt gaccaatgat gtgtaaccat
    26941 ctggcgaaat atttaaagta taaaaccatc ctgttttaca gtgtgttttt tagtctttcc
    27001 gcccttcaat cagcttttgg acaggtttcg gacaccagca cgctcactga agcaggatca
    27061 ccggaaccca tctgggtgct atcgcccgcc gggagtatgc aaaaaataac cgaagctccg
    27121 gccctggttt ccttagtgtc caacgatctg acccagaaat acggatggct cagcgtcaac
    27181 gagattttgt acagcctgcc cggatttgcg ccctcccagg attacgaccg aaggaccgtc
    27241 tccttccggg gagtgtttga gggttggaat aagaaccata tcctcaccct ggtggatggt
    27301 atccctttta acgataatct ctatggcagc gcatacacct gggaaaacag ccccctggtt
    27361 tttaccaaat cttatgaaat cagccgggga cccgggggct ccctatacgg gggaaatgcc
    27421 ataaacggta ctgtcgcagc caataccctg gaagcgaccg acctcgatgg gattgccagg
    27481 gtgcgcttgc gggcgggttc aaacaaaacc ctcatcacgg atttcctcac cggaaccgaa
    27541 aataaatcgg cgggcatcgt agcttccttc acccgctttt atacgggggg caatgaatac
    27601 gaatcctatg atctttcagg gcgaacccgg acgaatgctg gtctggaaaa atttaaaatc
    27661 agggatgaac aggacaacag ctattttttt gccaaagctt acggcaaagg aaaatacaaa
    27721 gggctttccc tacagtacca tgagcaacat tgggaatttc agaccggtca tggctggctc
    27781 ttttccgtgc cggaccaacc cgagtccatg aatgaatacc ggagggtact tgcctttaaa
    27841 tattcaccag gccgggaagg caagagattt aactacgagg ccctggcgag gtaccaggat
    27901 catggggtac actggaacat gcgttttttt ccagaccgca gcaccgccta tggccttgat
    27961 tttcccaacg gagtatccga atcgctgaag accggcgcaa aagatttttt tatccgtttc
    28021 cagggaacct attccctgga ttcaagcaag ttcctgtttg gtttggacgg cgaccggttt
    28081 tattatcgcg gtgatgtgtt tcatacggcc aatatggacc tgaacacgtt cacggcgccg
    28141 gattcttcaa accggacgta tacgctgaat ccgtggatgg aatacatatt ggataaaccg
    28201 gtacaccatg cagcggcctt tgtccggtat gtttcaccga tgtggttcga taaactccag
    28261 ctcatcctcg gaggacggta tgaacaccgg ttttttaatt ataccgatat ccaatccgcg
    28321 ggaaggccga tcaggcacaa aagttttaat ttatttgctc cgcatgccag cctgaccttt
    28381 catgcgggcg atctggtctt gaaagcgatg gcgggaagga gcttgcgcag cccatcgcca
    28441 acggagatgt tcggggccaa tacctttacc ctggcatcca atatcaagga actggaacct
    28501 gagatcctga cgaattttga cctgggcctg gactggaaat ggtcggaatc cctgaggttc
    28561 ggtttaaacg gcttcctgtt tcattttgaa aatcaaatcg cctacagcgt agccaatgcc
    28621 aacctgagca ccaatattta cagtttaaaa acggcgggcc tggaggcgga acttcatttc
    28681 accggaaacc gcctttccgg ttttttcaat tattcattca gcaaacgcct ggatgaatcc
    28741 atatccgatt cgaccgtagc catcagcagg aaaaaactca cctgggcgcc cgcgcatatg
    28801 gccaacctgg gcatggtcta ttcaataccg aaattttacg ccgcgatcgg cacgcatttc
    28861 cagggcgaag tggccagaag gtcctccgat atccttgaag cctttgaagg gtaccggccg
    28921 gccgccgttt cttcctgggt caatctggac gccaaggttg cctataaatt aaatccctcg
    28981 gtggaaatcg gcctttccgc aaaaaacatc accggccagg gacaatacct ggtcaaaaac
    29041 aacgcggctc cttttgatta ccgccgggaa ggaagaaggg tgtttatgga tctgagtttc
    29101 cggttttagt ttaaaaccgc ctgggttgat cttaaaacct ctttcctctg tgcgtcgacc
    29161 cttttcatga tatacggtaa gcccggttga aaaagtaaaa ttaaaaaatt ttattttaga
    29221 gatttaggag tattcgaaaa ttaattattt tagtaatcga atacaagcga taggacccag
    29281 gaaccccttt cccttctgct tcctcaccct ccttgcttgc agaacctgca taaaatgaat
    29341 aactatgaaa tatttgtttg ttattttaaa ccttcgtctg cccaccatta agtgggcaat
    29401 ctttggggtt attctgccat tgcttccggg attccctgcc ttggcgcaaa aagaaatccc
    29461 tgcattccgt tttggaaaat tgccggcatc caattttata cccaaggtgt gtccaattga
    29521 cagcgcggcc catgcttatt atttatttga tcatggccaa tccacctttc tcattacccc
    29581 ggaagtgttt aaagtggttt tcgaacgtca tttcaggatc cgtatccagg ataaatccgc
    29641 ctttgatcag gcggatatat ccatcccctt gtattacaaa gaccccaaat cagaggaaaa
    29701 actcgttgaa ctcaagggga tcacctacaa cctagaaaat ggagagctgg tcgaaaccaa
    29761 actcgaaaag tcatccatat tcaaggaaaa aacaggggag catcaaaacc aggtaaaatt
    29821 taccatgccc aacatccggg aaggaagtat tatcgaagtc ctatacaaaa tcgaatcgga
    29881 tttcctcttt aatttccagg gttgggtttt ccagcacgac atcccagtgc tggaaagcga
    29941 gtacctcacg gccatcccgg agcaattgca gtataatcct tatttctggg gatacctgaa
    30001 ggtaaatgta tcgcgtgaag agcgaaaaaa accaggatat gatttttttg aaatcctaaa
    30061 cggttatctg atccggaacg ttcccgcatt tccctcagaa ccctatattc acaataagga
    30121 aaactaccgg accagggtgg agtttgaact ggcttccacc aaatttggaa ttgattataa
    30181 agaatatacc aaatcctggg ctgccctgaa taaactatta ctggaagacc agcaatttgg
    30241 cttgcaactc agtaaaactg ggtattttaa agacgaagtg gccgagatcc aggctaattt
    30301 ttctcaaccc aacgataaga taaatgccgc ccatcagctg atacaacagc ggatgcgatg
    30361 gaatggggac cacggtattt ataccaccgg aaatatccgc aaatcgtttg aacagcggaa
    30421 aggcaatgta gccgatatta acctttgcct ggtttccatg ctcagggagc tgggactgag
    30481 tgcatttcca gttgtactca gcactattga caatggggcc attttgccaa cgttaccaac
    30541 gttgactaaa atgaattatg tattggctgc agtgatccag ggagacaaaa aaatcctttt
    30601 ggatgcagcg gaccctttcg ccacgcccag tctactgcct ggaaaatgcc ttaacgggca
    30661 agggaggctg atcaacgatc aggatggggt gtgggtagat ctcaaaactt ccaaaataga
    30721 tcaatcgcaa gcctattata aactggatct cgatccccag gatgggttca agggcaccta
    30781 ccaggaaagg cattttaatt attccgccta taggatgcga catcaattgg gtgattccac
    30841 cgacttgacc gagtatttga aaaacatcca cgaaaaacat cccggaacag aactgacgga
    30901 agcgatcatg accaatttta atgactacca aaaagacatc atcctcgacg ccaagatcaa
    30961 aacccgggga aatatggaag tgactgatga actgatttat tttaccccga tacaaatgga
    31021 aaagctggtg gacaaccccc tgaagattga ggaccggcaa tttcccgttg aattcgaata
    31081 tccctggctg gtcaatgcca ccgtagaaat aagaatcccg gaatcctatg atctcgaaag
    31141 caccccaaaa tcggccattt tccagaatgc ctctaaaaca gcaaaattta ccttcggagt
    31201 tgaggttgtt tccgggaata ctttgaagat cagttccctg atatttctga ccaaagcaca
    31261 gtacgaagta gacgaatatg aaggattgag ggattttttt aacctggtgg tctccaaaca
    31321 cgcggaacaa gtggtactga agaaaaaggt ctgatcatga aattatttta tccttttatc
    31381 ttttgcctcg tttctccttt tctgtatgcg cttcccgcaa cggaacctga ttacattacc
    31441 attcatcatt ccaaaaccca ttataaaatc aacgcgttgg acgatgtgga agaatccgtg
    31501 tggaaaaact ataccattca tcacgaaaaa gggttaaaag aagctacgct gatcctcccg
    31561 tatgataaac aatcaaaaat aaccgaagct gaaatcatgg tcaaaaacaa gtttggaatg
    31621 atcaccaaat acaaacttcg cgatttcacg gatcaggtga tgcaggacgg atattcgatt
    31681 ttttctgaca acagattaaa gtatttaaag attgaagcaa ccggctttcc aatgaacatc
    31741 gatttccgct atactataaa atataacagc accctcggcc tcggccggtg gatgccgcag
    31801 gaggggtatc attacaggct ggaacatgcg gaattgacgg ttcaatcgga gattccggac
    31861 ctggtatcct ataaggaagt gaattttcgg ggcgcccgcc aggtcgaatc caaatccctt
    31921 gcctgggtga cggataccct ggaggctatc caggacgaag acttcgacac gccaatggaa
    31981 gaaaagttca gcatggtgtt gttggcaacg aatcaaattg agttcgaagg tcaccggggc
    32041 gacctgggcg actggaatca gtacggcaag tgggtgaatg gcctgatccg ggacagggac
    32101 caattgcctc ctgaggacca acttaaaatc aatgccttgg tcaaagccac cgattcgccc
    32161 aaatccaaga tcaacacact ctacaagtat ttgcaggaaa acacaaggta tgtgagcatc
    32221 cagttgggca tcgggggatt tcagcccatg gcggcctcag aggttgcgaa agccggatat
    32281 ggagattgta aagccttgtc aaattatatg agggccttgt taaaacacgt ggggatcaat
    32341 tcttattata ccgaaatcgg ctccggaagc cgtaaaatca atcacaccga cttcagcagt
    32401 ctatatcaaa ccaatcatgt gatcctgacg gttccaggcc ctgaggatac tttgtttttg
    32461 gaatgtacca accaatattt cccaccagga tatctcggca atgccaattc agaccgatat
    32521 gcgctcatgg cttgcccgga aggcggacgg cttatccgga ctcctgtata cgacgaaggc
    32581 agtaataccc tgacttccca tagcgaactc aggctggatg cttttggtca tggcgcctct
    32641 ttcaccaccg cacgatatgg cggccttttt tatgaaacct atgccgacta tgacctaaaa
    32701 ggggaaaagg agaaaaagga acaactgctg gaaaatcgca aaggcatgga ctatgccctc
    32761 gattccttca attacttttt tgactacgcc tccgtgcccc ggatcgcttt caatgaatat
    32821 gcccacatca aaaaatatgc ctccgtctcc ggtatcagga tgtttatccc cctgcaattg
    32881 atccatgaat tggccccgga tctaataccc ggtaaatccc ggatgaacga tattgtgatt
    32941 ccttttggat tttcccaaaa ggatacttta tcaatagtca tcccatccgc ttatgaaata
    33001 gaaagcctgc ctaaaccttt taccattaaa tcggtgttcg gggaattgga ttttaatgca
    33061 acggttatgg accggacggt acagatcaga cttcatttta ccttgctcaa aacacggcga
    33121 tccaaagaag cgtataccga attttcggat ttattgtcga aagcttccaa ggtcaggcaa
    33181 tccaaaatag tgatcaagaa gatagaaaaa ccctgaaacc atatttaata ggattatagg
    33241 attataggat tgatgggatt attaggatta attggattga tgggattgat aggattgatg
    33301 ggattgatat tgcagtttac aaattggcgg aaacaggttt ttcgctttta aatcttcgta
    33361 tcaaaacgag ggttccgtaa gccaaaacca atagacccgc caggaacagc ggccagttga
    33421 aagttgaggg aatataatca gacagaatgc cgtccaggta ttgggacccg gaaaggacat
    33481 ccgacctgtc catgaagctt attgccctta ggtatttcca gaaatcccag acaaatgaga
    33541 ggatgactac caaactgcct gcggcaaaca ggccccattc attggctttt aaccgcgtgt
    33601 ccttttcctg tgagtgataa taaaagatgg ccgaagccaa aaggatcatg ttgaggcata
    33661 ggatgcaggg agcaatcacc gggcccaccc agggaacggg aattaaaaaa agaatatccc
    33721 aggtgaaaaa ggattccggc cagtccagga ggatccatag aaacaaataa taaaacaggt
    33781 cccagatcgc aaaacaaaaa atgaatacgg caaggcggag ggaagccttg cgggctgaaa
    33841 gcatgcctat gcccagcaac atcaggatgg tagccgcttc gcgcaacaat tcaaccatcg
    33901 ccatttctgc ctccatgggc accaggggaa atttaaagcc gtccggataa tacaatttcc
    33961 gcaggtagac cacgaccgcg gattccagat aacccatggc tatgctgaaa acggcaagcc
    34021 agatgatcgt tttgattttc attctacccg gagtcatcgt cctcttattt gaaaccgaac
    34081 aggaccggga aaataaaagt caccaccagg gcccagagga cgtaaacagg caaataggaa
    34141 gcaatgcttt tttcgacggt ttccgcttcg gttttgttcc ggatggtttt taaaagacgg
    34201 taactcacca gcagtaagtt gatcagcatc aggagatttc cccccaggac ggcaagccgg
    34261 tttggggtaa tgccccactc ggaaatccgg aacaggatgg cggacagggc aatgccgttg
    34321 atcaggatgg ttaccccgga aagggcaaac aacatcattc tctggccgtt gcccccgttt
    34381 tttgaacttt cggcaatgga aaaaaggatg atcgccatga ccccgatcag caaggcatta
    34441 aaaatcaaaa gaaattccct gtcgttaaag gggtctttgc cggtgtatat cacggcaacc
    34501 agatagatgg tcagcatggc cagcaccagc ggggtaaaca cctgggcaat gaccggcgaa
    34561 acattcttta ccagttgcgg gttgatctga ataagataat ttccgagaat gggagccgcg
    34621 gcgaggcccc agatgccaaa ataatggaaa tacaattctt cgatcttaag gtcgatgagt
    34681 tcaaacaatc caatggttac accggtcagg agggcccccg ctatacctat gatggtagtc
    34741 atcaccagca ggtctccatt atacctcagg aaatccaagc gccggtaagg attgtttcgg
    34801 tccccgccaa caaaggaaat gcccagcacc gcccacagca aaagcggcag atgaatgcag
    34861 gccaggatca gggtatcgct ttccggatta tcgggaagca gatttatata tcctgcagac
    34921 ccgagaatca ccgccaaaag aggcaaaaac gtttttggag ccagtttttg tttccagaca
    34981 aaataggcgg acaagaaggg gaaaacaata aaaccgaggt tgcggggata aaaaaaatcc
    35041 tggtcaagcc atgaaaaatc agggattttt gccaccaacc cggccaggaa ggcagccacc
    35101 aggataaagg tccattcttt tgaattgccc caggaaagat ccccgttttc gaaattcaat
    35161 cgttcatgcc agtaaggcac catcggcttt tcacggatat ccgggtacag tcggttaaaa
    35221 gcttttttga aagccttttt attgatctgg tacaattgct ccagttgtct gggatagtcc
    35281 aggtttgcaa ggatttcatc ctccaaaggg aggatctcgc caggattgcc ggtttttgat
    35341 tccataaaat tatttattaa tagtgctttg aatttcaaag tggatgtata aaaaaaatca
    35401 gcccgatttt ttaatcaggt tttccaatgc tttcaggtgg ttctcaaacg ctttcttgcc
    35461 tttgggggtc ataaaatatt ttgtgttggg ttttttcccg ataaaggatt tttccacccc
    35521 gacaaattcc tctttctcca gcgctttgat atggctcgcc aggttgccgt cggtcacctc
    35581 gaggaattcc ttcaaggcgt tgaaatcaaa cttgtcgttg atggccaggg ccgacataat
    35641 gccgagcctg atcctgcttt caaaggcctt gtggaacccg tttattaata atttcaccgc
    35701 tcgtacctgt aatgtataaa aataccgtaa agcacatggg ccaggccaaa acccagggcc
    35761 cagaacaaca aaccatactc tataaaatag gcaccggcca ggcccaggac catctcaata
    35821 agccccaggg ttttcaactc atcataggta aaccggctga tatggtataa agccagccca
    35881 taaaacaata aggtaagcgg ggccatcagg ccaagccatc ccttggatat taaaataagg
    35941 atcaacagcc cacccgcgat gagcgggacg gccatatggg ccaataatct gcgcgtggtg
    36001 gcgttccaga ttcgctcacc ccttttatgg gctttctgtt gggaaaaata aatggccgtg
    36061 ccgacggcaa ggatcaggat gatcagggct aaaacaacaa tcttccaaag gtcgaaattt
    36121 aagatttccc ccgggacctg gcggtattca aggtcagcgg gattaaatcc aagaaaggaa
    36181 taggcaatat aggcgcccgc cagagcataa atccccgcca ggatgccgga ccagccggaa
    36241 agggataaaa acttggaaga acgctccatc atggaacgta tctcggtgat gtcccgtaaa
    36301 taatcctgct cttccttcat ttaaaagtac ttttaaatac aaagtaaaca ctaaatttta
    36361 atgcgcgcaa ggcctgtccg caaattttta aaaaaattcg aatggaaaaa tgcaaaagca
    36421 gggttgctta aaattaaaag ttgggctgtt cggtttactc agaaagggat ttcttgaata
    36481 cccgttgcga cctgggtgcg ccgccgtaat aggctttcca gatcctgctg aattgccttg
    36541 gactttgata tccgcattgc ccggcaattt cttttagggt caattcggta ttccgaagca
    36601 ggtgttcacc gcgttccagg cgaagtttat gtcttatgct ctggatgctt tcgccggtgt
    36661 ggtgcttaaa caacctggaa atctggcgca cgctcatgtg caggtctgca gccagatggg
    36721 ttaaagtata ggaggactgc aggttgttga ccagcaactg ctgggcctta tagacttccg
    36781 ggttgaaatg gtttttaaaa tcaagaaagg tatttttttg attttgggtt tctgcgcggc
    36841 gcatattgat gaccatctcc tgggcgattt ctgcagaaac caaaggattg gaccattttt
    36901 cgaccagggc cagggccata tctataccgc aagtcatgcc cccgctggtg aatatgcctt
    36961 tgtcaaatac atacaattga ctatccataa accgcacacg gggaaatttt ttccttgcat
    37021 aatccaggca tttccagtgt atggtgcatt tggtaccgtt caacaggccc attttcgcca
    37081 ggatcagagc gccggagcag atggaggaca aatacacccc ctttttataa tttttaatga
    37141 cccaggacgc ggtttgttca atttcagcat ccatctcccc gcggacaaaa cttttaaaat
    37201 caaccccgac taccacgatc atatcctgtt tgcgaagagg caccgcttcc ggaggacgga
    37261 gcccggcaaa ccggagccct tgttcggtgc tgatgctttc cttaagcgaa acaaaatgaa
    37321 gttggtattt tcgggtacca aaactattgg cttcataaaa cacctgggcc gcaccggcca
    37381 ggtccagtaa gtgggctttg gaataaagca taaatactat atggattgga tccatcggtt
    37441 ttcaggttgg aaagcgccat cgccttccgc gaaccggcag aaggcgaagg tcataaacgg
    37501 acaaatttaa gtcaatcgaa gacatattgc gtatggggga ccgggaaccg ctaaccttgt
    37561 gcggtttctt ccggccagag gatttcaatt ttagcagggg aaccgaaacg gcctatgtat
    37621 caaccgcctc aaattttata acatgaaaaa aatcattttg ggatgccttg catggatcct
    37681 ctggaatgcc gcgcaggcac aattcaaaaa cgacaacctg gtttataaaa ccgtttatct
    37741 ggaggacttg tgtcaatccg tcaaacaact gaacggcgga ttgctgttgg atgtgcgttc
    37801 tccgggggaa ttcagcgata cctctgcgta tcacagtttg aatataggca gattaaaagg
    37861 cgcggtaaac atcagttcca gggagatcca gaaacgactg gatgaaatcc gggattatcg
    37921 cgacaaaccg atcctgctga tctgttcaca tagccaaagg agccgggtgg tatccaaaat
    37981 gctggtggac agcggcttta ccaatgtgac caacgtcaat gggggaatga cggaattaaa
    38041 ccggttgcgg aacggcagca ttccctgttt gaacgaactt tacacctccg gaaacgaatt
    38101 caatttattg tctccggccg gggcttttaa tttggttcgg aacaccaggg acctgtatat
    38161 tctggatgtg cgcccggaca ccgcctttca gggcaagacc gcttccgcca tgttaagggc
    38221 ccatgggcga tttaagaaag cggtaaatca accgttgatt tcattgaagg aaaacacgga
    38281 taaaataccc agggataaac ccatactgat ccttgatcaa ttcggggacg acgccgtgca
    38341 ggccgcccgg atcttggcgg accgaggatt taaggggatt cacgtgctgt ttgatggcct
    38401 ggctgcctgg ataacggaaa cggaaaagga ccttaaggaa agaaagaaaa tttgggaaaa
    38461 gggatcgtcc tatggtctga tcaattcgca ggatttgcac cgcaggttta aaggggcata
    38521 ttcgggcctg attctggata tccggcccta tgccgatttt aacaatcaaa acaagaatca
    38581 gtccttcagg aacaagggcc atatccgggg agccatcaat atcccggaga acgaattaaa
    38641 atcccgtttg caggaactga cgccctataa gaataaagaa atcatcgtct atgccttttc
    38701 aggaaatccg gaggcctttg ccgctgcaga gatcctgatt aagaatggat ttaaaaaggt
    38761 taaggtgctg accggaggat tatttgccct gaggtgggaa gcggctaatc ttatcggaat
    38821 gaaagagatg atgaactggg tggtaaacgt gcctcccgaa aattattgag aaactgctca
    38881 acaaagcact acatttaatc ctgctcatca tgcaggaacg gaccgccaga ttcaggaaaa
    38941 cgctcgattt tttttggaac atcagcgaca aactggtact aaccctcatt ctgtttcagc
    39001 tggtttattt cctgatcctc tatttttctg aactgaagga gagaaaggca gaactgatcc
    39061 cctttttaac ctggtttatt gtttttcttg cggccaccgc ccttctggtg gggatttata
    39121 aaatatattt ccagaaaaag gataccataa ggcgtgtggt aaccgccttt tacagaattt
    39181 ttattgttgc ttatttattg caatggtttt ttgtgttgtt gtactggttt atcaatccac
    39241 cgctcacctt tacgcagatt tcaagctgga ttgccggcca cgggttgaaa agggattatg
    39301 tggactatga cgccatcagc cccaacctga aactggcggt cttggccagc gaggaccagt
    39361 tgtttccgga ccatgacgga tttgatatca aagcgatcaa actggcctta aagtataata
    39421 aaatcaggcc caagagaagc aagggggcga gcaccatcag ccagcaaacg gcaaaaaatt
    39481 gtttcctctg gcagcggcgc aactttattc gtaaaggcct ggaagtatat tttaccttta
    39541 tgatcgaaac cttttggtcg aagaaaaaaa tcctgaaccg ctacctcaac gtcattgaaa
    39601 cagggaaagg cctcttcggc atggaagccg cggccaggta ttatttcaat aaaccagcca
    39661 aagccttaac caggacggaa tcggccatga tcgccgcctg tttacccaat ccccgaaaat
    39721 acaccgtcaa acccatgagc gcttatgtcc ggtccagggt tgaatacatc ctcgttcaaa
    39781 tgaataatat agaacctgac cccgacatcc agtcattgat cagatgaaaa aaaggaattc
    39841 cttcaagaca gtccccacgg aaagctacaa actcttaatt gcctttttca ggtgcgcata
    39901 actgatttta atctcatcgt taaaatgttc ccatttaacg ccagcgtcgt gttgcctgga
    39961 ttttacttgg gatacgatgg aattgacttt tttggcggca tcatacattt cggccttaaa
    40021 ctctttggtc agtttctttt tttccgcgcc cattttcttt tcaaaaagat ccatttgcaa
    40081 tttcagtttt tccattgcgc cggtaagatg gcctgcgaat tttattaatt caggtctttg
    40141 ctttaataaa tttttgagtt tgtccaaagc ctgaatgata ttcttcttct gttcctggaa
    40201 caacactttt gtttttgcct tccctttatt gagcatctcc tcaaggtggt cgactctttc
    40261 attgagggac ttgaaccagg tatcgttgag ttgagcaggg ggtggaagga gttttttaaa
    40321 ttcctggagc cgtttcttca tctggacctt ggcttcttca aagagatcaa tgactttcga
    40381 tttacccaag gcgatttgca ccgccaggtg atccagatcc tgctgttctt ttaataattg
    40441 attggcaaga cggaccaaaa gggccttatt attcttttca tcagattctt tcatattatt
    40501 agattttagc aaaatgtaaa attaccggaa gatgggatcc tgaaacctga tcagaatcag
    40561 gtgacggggg cacctttctc atatccaatg aatgaatctt cttcaggaaa gataatcctg
    40621 ttcaaggatg gcctgcaggc caagcatgat ctcttccagt ttttgcttta tggaagcaaa
    40681 tccttccaag gcccggatgc ttaaaggagg attcccggct tcggtttcta tttgagacag
    40741 gggaatcagg gattcgtcca gtttgaaaag cgcaaaggtg gacttcaact tatgggcaat
    40801 gttttttaca gtattgtagt ctggttcctg aagcgctttt tcgagtaatt caatatcctg
    40861 aggtgttttt tgtatgaaca gttcgatcat ttctttttca aaggcagcat tcccgttgga
    40921 cagttctttt agataagaaa gatcaacggc agcggggacg actttttggt ctgcaggatc
    40981 aggagcaggg ggaaggtcat gggaacggtc ggccccaggt cgattcacca gtaacgcctg
    41041 gattttttca aaaagatctt cctgtttaaa gggtttgggt acataggcat tcattccgat
    41101 ttcgaaacat ttctgctgtt caccgaccag ggaatgcgca gtcatagcga tgatgggaat
    41161 gtcggattta aggacttccc ggatatgatg ggtggcctga taaccgtcca ttaccggcat
    41221 ttgcagatcc atcaggatca ggtccacctt ccttttgggc agacaatcga tacattcctg
    41281 tccgttgttg accacggtta tatcaaaacc aaagttttca atgacctgtt tggccaggcg
    41341 ctgattgatc acgttgtctt cgcacagcag gatggaaagc cggcgatccg acttcagacg
    41401 gaccaccttt tctttgactg actcctgctg ttggtccgcg gtttttttat attccaggat
    41461 aaaatagaat tcggagccca atcccggttt gctttttaca tgaaggctgc ctttctgcag
    41521 ttccaccagt tgtttggaaa tgttcaaacc caatccggtc ccgccaaacc ggcgcgaagt
    41581 gctgtcttcg gcctgggtaa aacggtcgaa tatggtactg agtttttctt ccgggatgcc
    41641 gatgcccgtg tctttgatgg aaaaccggag ggttacgctt tccttcgtct cttccagctt
    41701 ctttactgtg atgatcacct ctccggaaag ggtgaatttg atggcattcc ccgcgaggtt
    41761 catcaaaatc tgattcaacc ggcctttgtc tccgatcacc atatccggaa tatccggatc
    41821 cataaacagg ctgaattcaa gatccttgtc ggcggccttc accctgagca gtttgtagag
    41881 atgcgtcatg gcgtttttaa ggctgaaggg atttgcttct atgaccaggt ttccggattc
    41941 gattttagaa aggtccagga tatcgttgat gatcagcagc aaatgatcgc cggcattttt
    42001 aatgttgtgg atataatccg actggacctc gttcaggttg ctctggacca ggaggtcggt
    42061 gaagcccagg atggcattaa ggggggtcct tatttcatgg ctcatattgg ccagaaaact
    42121 gtccttggcc aggacggctt tttctgcagt ctgcttggcc tcaatcaatt ccgcttcggc
    42181 ccttttttct tctgtgacat ccgattcgat ggcaataaaa ttgatcagct cccggttttc
    42241 attaaatacg ggattgatgt tcatgcgaag ccagtaagga cggccacttt tgctccggtt
    42301 tttgatgtct ccgtaaaagg agtttttttg ctccagggct tcatggattt gcctgaccgt
    42361 ctccgggtcg gtctcgtcgt actgtagtaa ttttccggga tccttgccca gggcttcctc
    42421 caggctgtac ccagtcaatt tttcaaaagg ccggttgacc cattggattc ttttttcttt
    42481 gtctgtaatg acgatgacat tgtcggtgct ttcggcgacc agagccaggc ggcggtattc
    42541 ttcgttgagt ttgcggattt cggtaatgtc cctgccaatg gaaaagatag tccccgtttc
    42601 aaggtccgaa gaggcgttcc agctgatcag gcggaacgat ccgtcccgca tttgaagttt
    42661 tgtttcgaaa ttccgggagg gaatgccttt ggatcttttt tccagttcct gctccagggc
    42721 aaggggaccc gcatcccggg gagtggcaaa ggcggtaaaa ggctgggaat ggatcagttc
    42781 gggaggaatg cccagttcat tgataaactg agggctgacc ttggtcagat atccggcagc
    42841 attcgcaatg cacaggtaat cctgggacat ttggaaaaac tggtttaggg tgttttcctc
    42901 ctgtttgatc cgggtgatgt ccttgcaaat ggagaagtaa cgataaggtt tgcctatttt
    42961 attaataaaa ggaaatatcg tcgtgtccag ccagaaatac gaaccctctt tgtccttgag
    43021 ccggatgtga ttgttccaca ccgatcctcc cgcaatggta tcccagagcc ctttgatgaa
    43081 ctcgggggaa tgtaatcccg aactcagcat cctgtagtcc tggccaatga gttcttcccg
    43141 gctgtatttg gaaattgcgc agaagaggtc attgacataa acgagttttt gatcttcatc
    43201 ggcgatagaa atgatgtggg tcttttccag gcccgttttg atgaggtcca gttccatgga
    43261 aggggattcc attaaagcaa acctgcttaa ttcagaagaa ggaacggcgg ggccgggaac
    43321 gggtttatgg cttttactca ttgattcgat taaagtataa aaaatgcggc atttaaatta
    43381 aaaaaatcag aatccggaaa ccggcttgac ctttcagaaa cacaaaccgc tcgccggcaa
    43441 tccatcctga cgtcaagcgc cagccggatg acctggaatt aaaaatcttc ttcaaaaggt
    43501 tcgtccccct ctcctttatc ctttttctga ttgagccggt aagttaaact gagcataagc
    43561 tgtcggcgcc tgccctggaa ctcgctgtaa gaattcaatt ccacccctgt gatttccctg
    43621 cggaaacggc gagtattaaa gaggtctcgc acactgatgg ccagggttgc atttcccttc
    43681 atgagatccc gcgacagggc aagatcggtg gaatgtaggg ataaccgtcg accctgggca
    43741 gtgttttgag gggcctggta attaaaagta ccctggatat cccatttttt ccggaccgta
    43801 aactgcaaaa tggtccggtt tgtccaggtc agggcttccc tggcaaaggt ttggttctca
    43861 taaacccctt cagaacttac ccggtagaaa ttgtaattgg aagtggatcg aaaccagggg
    43921 cctggagacc aggataaatt gaattcaaaa ccaaaagccg cttcacggga gagattgatg
    43981 gggaattgac gactaaaccc aatggaatcc accaaagtga tctcttcaat cactccgcgg
    44041 cggccgcgat aatatatact ggaaagcaaa gaacccgaac tccagttgaa caaatgggaa
    44101 ccttccaccg aatgggtaaa ttccggccgg agattcggat tgcctgttga cagggaccgg
    44161 ttgttggaat agttggagaa aggcaacaaa tgacggaaac gcggcctgct gatccggtaa
    44221 ctgtaactga gctgaaaagt atgcgattcg gaaagtttgt aggaagtaaa caggctggga
    44281 aagaggttaa aataattttg acggttgccc tcaccggttc gtttcaggtc aactgaaatc
    44341 ccggtaagtt ctccccggat accggcctga taggaccaac gtttccaggc ctttccggcc
    44401 atggcgtaac cggaaaatat tttttccgag tattccaggg cgttgttgaa acccgaaagg
    44461 atatcccatt cactgaaatt gttttctact tcgaccagaa attcattttc aatcacccgc
    44521 aaactgccgc gcaagccggt ctcaaacttt ccggtttcgc tgaaaggctg gacataatcg
    44581 gcctggaaaa aaacatttct ttcattttcg gtgttggaag acctttgcct ggccaccgtg
    44641 ccgtcgcggt catcggtttg ttcaaaactt gaaatttctt tctctacatt ttcgatccat
    44701 ttgaaatcgc cgctcagttg ttgtccctgt tttttaaaat cctttcggta actgagtgaa
    44761 acctcaccgt tggtttccgg ttcctcttcc ctttccctcc ggctgagggt ccgaagcaac
    44821 cggtcgttcc cgtccaggtc acggaaccga ataagactgg tattgagccc cgaagaggga
    44881 cggtatacaa aggatcccgt gagcacgctg ctttcattga aataatagtc caatcccccg
    44941 atcaggttgt gggaatgacc accccggacc cgatcggttt cttccgtata ggcaaaaacg
    45001 gaatccggca aagtataacg gaccgatgaa atccctgagc ccggactttg acggtaattg
    45061 aatccgtaac tggtgaaaaa attcagtttt tccctcctga aattgaggcc caaagtcccc
    45121 ccaaaatggt cgggtcggcc ggcattcagc atcaaagaac cgttgacgcc ctcccgatgt
    45181 tcctttttaa gtataatatt aatgatgccc acctctcctt ccgcgtcaaa ccgggaggaa
    45241 ggattcgtaa tgacttccac gctttcgatc aggctggcct gtacctgttg cagggcgtcg
    45301 gcgatattga ttccggtgag gccggagggt ttgccgtcga tcaggatccg cacattttgg
    45361 ctcccccgga ggcttacgtt gccttccacg tccaccgtca cactggggac attgttgagt
    45421 atatccgagg cgcttccgct gatgttggtg aggtctttgc ccacatgaaa aacccttttg
    45481 tccaaacgga gctctgtagt gctccgttcc ccttcgatga tgatttcctc cagcagggca
    45541 ctggcctgga cgagggtatg acttcccagg tccagatccc ggctggccag cagtatcccc
    45601 ggaatgaact tttccacata agataaaaaa gtgatcttaa gaaaaaaact gccgggcgga
    45661 ctttcaattg aaaatattcc atcgtcatct gtaattcccc cgctcaccag tgtggaatcc
    45721 gcggaccggt acaatcccac atttgcatac gcaatgggct ctttgttttc atcgttcaca
    45781 cggcccgtaa tggaaaaccg gcgttcctgg cccgttgccg gaaggaggaa caggatcatc
    45841 agtaaaaagg ttatcggtac ggtattatac atttcgaccc ttcctgttta atgattattg
    45901 cccggttgcg ggcgacgcaa accggcaagt taaaggttat tcattaaacg ggcgggaggc
    45961 ctttgtggtt taatggtatt gaatataaac cggccggggt ttgagttgaa ggatctgttc
    46021 cggtgacatc agggcgtaac cgtccagcgt atcattttta taaaacaatt tgaatccggc
    46081 aaactgtacc ggttctttat ataccgcctg gtaataagaa tttctttttt tggccggaaa
    46141 accaaaaccg tccatattta tgacgatctg cacttcgggt cggatctgga tgtggcggta
    46201 ccgggtgagc attcttttgt tgaagcggtg aacgaccagg attttcggag ctaactggta
    46261 ttgcctgacc aaacgggcca ggaattggct tgccgtattg atttcttctg catcgacaga
    46321 tccgatccta tcccccggcg ggctatcctc tttcatggaa aattcaggat cgatcccgag
    46381 atggacattg gtttggagca gccagggctc caggtaatgg atttcatcca ggagggaccc
    46441 cttgcccacc tggacatcca ggaaaaccag gccgtttatg gctttagcca gatcgagagc
    46501 tctttcgatc tgccccgggg gcatcctgag ccggtatttg gaatcccgtc cggcttgttt
    46561 ctgggcggag accgctatgt aatgaatggc cggaatgacc ggataagaag gatccgccgc
    46621 attccagcgt tgcatttcag aatttatttg atggatcagt ttgtcgcggg gcaaggcccc
    46681 caggactccc attttcgggg aatagaaatt tccgtaaaag gcgaggatcc ttttgaaagg
    46741 gaggactgct ccggccaggg gaagaggcgc tgcggcgggc catagcccgg aagaatcgcc
    46801 attggcaaga tcctggatca gccggtgata caacagggta tccagacccg ggtcttttac
    46861 ggcttttcca ggaacctcac gtagcggaat ttctgcgcgg accggtccgg atttgatttc
    46921 ttcttcggta caggctaaca gcaggttaaa taaaacaacc accacccagg ctgttttttt
    46981 attaaaatgc ggatgttgta aaacaaacaa gggcaaaaaa cgaaacacct tatcaaaata
    47041 aatcccccta aacaccattt tccactgatg aaaatcataa aaaggaatgt ttgattttct
    47101 ttgttggagg aaattcgaag cggggccttt tcaggaaaat gggggtaata tcggaagcta
    47161 gccttttata gtcaggcagg aataccacca gtggtcatgt ctgagataaa ggatataact
    47221 gccggcagca agatggtcca gcgccagggt gtttttagcc gccgtcaata cccactctcc
    47281 catgattctg ccggagaggt catacagaaa gcaccggacc ggccattgtc cttcgggaag
    47341 cgtgacgtga aggccggcat cgaatggatt tggataagtt aggatccgga aatccggttg
    47401 tccgtttaaa tagggggtgg attgattttt caggcttgaa tggatatgcc agaagccctg
    47461 gctgttttgt acctgccccg aggcgaccac ctggatgagg ggctgtgaaa ccgacccccg
    47521 gaggacgagg tttccgtccc gcatttcatg tcctcctgca ttcagggtat aattaggtaa
    47581 acgaagttcc tggccggcga ggctttggta tataagcagt aagattagga atagaaattt
    47641 tttcatatcc cggcccgatt agtttttaat gatcatggct tcgaggcttt ccagcctttg
    47701 agacagggat tccatcgatt tcatccggga ctttaattct tccagctgat ttttcaggtc
    47761 ccggatttca gcttgctgct cctggatggc ccggaccagg gagggaacaa aattgtcata
    47821 agtcaggctg taggtatcct gatcgttttt ggggaggtga aggcctgaaa aagcataatc
    47881 cagggcattt gccgcctttt ctacatcctg ggcgaggaaa ccgctgattc tgcgcgggtc
    47941 cccggcatgg gtcaatgtat aggatacggg cctgagccgc tcgacgaaag caaggccggg
    48001 aatatcctcc tttactgaag ttttaaacct agcatccgaa aaagtcgaaa accctacgtt
    48061 gcccctgatc tgggtgatgg ccgtgttgcc gaggatgatc tggtttgaag cggtgaccac
    48121 ggctcccgat ccgatcgcgg tggagttctg gacggaaagg ttgctggatc tcgcgttttc
    48181 accaatgaag gtggtattgg tatgggtggt ggacaggccg ttgaaaaaac cggcgaaatt
    48241 gccgatcccg acgtttccgt tgcccacatt attggtcagg gccaggttgc cgacggcgac
    48301 attgaaggaa gaattattgg aattgcgcat ggcttcaaat ccaatggcgg tgttgctgtt
    48361 gcccgtggcg gaggtcagca aggcggaatg acccatgacc gtattccacc ttccggtggt
    48421 attggaactg ttggaatgat tgcccaccga ggtgttaaag gaacccgtgg tattggagag
    48481 gaggctggag tagcccaggg cggtgttttc ggaagcggtg ttattgttta agctgaagcc
    48541 tccaattgca gtattcttat gaccagatac attgctcctg agggtttgtg atccgatggc
    48601 tgtattatcg gatccggtgg aattcagccc acctgcttca aaaccaagga ctgaattata
    48661 aaacccagtg ctgttttgtt caagcgtccc tgttcctacg gctgtattgt aactcgcatt
    48721 gttctgaaac aaagaacgtg ctccaacagc cacattctta gacccatcaa tacttagttc
    48781 gagggcgctt gctcccaggg cggtattgct ggaagtactg ctctgtgata aggctaaatt
    48841 accgatggcg gtatttgaac ttcctgtaat atttaaagcc aaggcgccag ccccaacagc
    48901 agtgttgtct tcaccggata cattggaatt tagggaaaaa attcccaacg cagagtttgt
    48961 ttttccggaa gaattggaag ctaacgaatt cacgccgaca gctgtgttac catttcccat
    49021 attggaccat aaagcagctg ttccaacggc cacgttattt actcctgata gactcttttc
    49081 catggccccg tagccaagac cggtgttgtt aaaccctcta tttgaaaaaa gtgaataagc
    49141 acccacggcc acattcctag ctccttcggc attttgttcc atgcttctaa aaccaatggc
    49201 tgtgttatct tcaccattat tttcatacaa agtcagaaat ccaatggcgg tattggattt
    49261 tccaagagtt gaattttcca tggagcggtt tccgagagca atattgtttt caccgttatt
    49321 attaaaaagt gcagtgtttc ccagtgcaat gttgttttta gcctgagcgc cttcttgcat
    49381 ggaagccaga ccgatggaaa tattcatttc cccgttattt gaatagaggg ctccttctcc
    49441 gatggcaata ttgttttttc cggttatgct ttcttgaagc gcggcaaagc cgatggcaag
    49501 attggattcg ccgttactgt ttaaaagagc atttcctccg acggcaatat tattttttcc
    49561 cgaagcgctg ttttccatag cgcggctccc gatggcaaga ttgttttccc cattattgga
    49621 tttcagggaa tatgtcccca gggcggtgtt gttcttgcct tggctgctgt tggccatcgc
    49681 ataagcccca acggctgtat tcatatcgtt tttattaatg cccagcgaac cgaatccaat
    49741 gccagtattt tttacaccct ggttttgggc caaagaaaaa gaaccgatgc cgacgttttc
    49801 attgccaata ttcttttcgc cggcgcgata tccccaaaac acattatcca ttcctagata
    49861 acccgccgaa gtgtccctga gcctgaacag gaggggccgg tcatcgaggg tgcccaaaaa
    49921 gtgcatcccg gtcaggccct tattgccatt caaactccag cctgaggtga cctggtccgc
    49981 ataaatcgcc cggatcgcgt aaggcgcgct ggacaaaggc gaacgcgggc tcatctcctc
    50041 acctccatcc acccgaaccg cgagataata aggtttgtca aaaagcaggc ttgaagggat
    50101 cggggtggtt tgaccaatct ggtaatggaa taccccgttg gaaagaaaaa cctgctgggt
    50161 ttccgaatac aacggattac ctccggtggc ctggtcatag atcaggacct gaatggtata
    50221 atttttatcc ggcaggattt tcccggcggc atcggcgaga atgccctggt aactgaattg
    50281 tttcgggacc tgggcctgaa ggagggaaga cgacaaaatc agggcaaaaa caaaaatgga
    50341 ttggtacata tggaaagagt tttattacca gaaaaagatt ctatggatgc ctaatataat
    50401 aaattgtttc tggttttgta aatagaccat tttactgtat tttgttttgt taacggcatg
    50461 cagtatggca ttaagttaac cgtagattgc cctttagcat aaggtcgaag ccagggtgat
    50521 cctgtgcgta aaaatcagat accaatgaat acaggcatct ctttttccag atcaagatat
    50581 ctggccatgt ttattttcct tgtaatcatg ctggctacgt ccatcggcaa gatttcggca
    50641 aaagacgtca atttgggtaa gatcagcgca gccgacctta aagcacaatg tgaagcggaa
    50701 gggggcactt tcaccatcac tccggacggg gaaaattgga tttgtaccaa aagatgcggc
    50761 agcagcatct gcctggtcat ttgtgatata gaaggaggct gttctggcca ttccccgggc
    50821 aaaccggcag atcaccggcc ggtgacgcat acggtttcag aggttctgaa tggaaagatc
    50881 aaggacggat ccggaacgat tataccatgg cttatccccg ggctcatcat tctcttggtc
    50941 ctgggcggtt attgggtctt aaacaagcgc agatcctgag gcctctcatt gacttggtga
    51001 cgtcaggtgg taaaaccgga ttaggttgat ttattgttct gatcaggggg cttcatttat
    51061 tcagcatagc cggaaaccat attctcctaa gtttgggatt tgataagaac cgttcgtacg
    51121 ggaaagggaa tgacaatgcc ctcctcccta aagcggcggt gaagtgtttt gataaaatcc
    51181 gaggtgatca aaaattggtc gccaaaggtt ttggagcgaa ggaaaatccg cagatcaatg
    51241 ctgctttctc caaactgata aaaacggaca aagggttcaa aatatttgac cccgcctgcg
    51301 ctttcttcca ggattttctt gcctgtctcc aggcaaaccc gctccacttt ttccaggtcg
    51361 ctgtcataac tcactccgac cgatacatta aacagcactt ccgggctgtt gataaaatag
    51421 ttctcaatga tcgaatcggt catcttactg ttgggcacga tgatgatgtt gttttgggcc
    51481 gtttcaatgt cggtattgcg ccaggtgatg tctttaataa aacctttttg gcccccttcc
    51541 aattggacaa aatcaccgat gttgatcttt ttggaagcga tgagttgcaa acctgcaaat
    51601 aaattggaaa gcgtggactg aagggcaagg gccacggcaa ggcccccgac tccaagggcg
    51661 gcaagcaagg gagtgatggc aattccgaat aaccggaggg cgagcatcag cccgatggaa
    51721 taaattatgg cccgaagggt attgcccagg atggaagtgg aagcatattc ccggtcctgt
    51781 tcggacgaga gggaatataa taagcgggtg gcgatggccg cgacgaccag ggtaatggag
    51841 aatataaaag cggtgtggat aatgcttcta aaaagggatt catgccgggc tgtaaacgcg
    51901 agggatcctc gtaaaaccgg aagggcgtac aagagagccg ccagcaaaga ccaaaagagg
    51961 atccaggggg agaccgcagc ggcgataata tcatcgcctt tccattttgt tttaccggcg
    52021 atccgcagca caaaagggat tactgttttt ttgatcagga accccaggag caggctgatg
    52081 gccaggacca gaaaaacttc gagcaggggc gagttgccaa ttccattcat ggtattgcag
    52141 ctgtctttta ttttaaaaaa gggttcatgc ttccaggcct ttgccggctt acgttgaaag
    52201 ggttccgggc gatgtggtga tcagccgtat ccccgcattt tccaggctga tctttttttg
    52261 tttatacaag gcgcccaggg ccattttata ggtcttttta ctcattccga aaaaagaata
    52321 aatttcgtcc ggatcgcttt tgtcgtgata gggcaagtac ccgccatgct cctggaggag
    52381 gcggatgatt tggtcccctc cttcttcaac ccgctgatat ccccgggcgc caagcactac
    52441 gtcgatcgtg ttttcagccg aaatgttttt tatatatcct ttcattttat ccccgatgcg
    52501 gaggtcgcgg tatatttcat tttggtgcag gaccccggta tgtctctggt tgatgatcac
    52561 tacatacccg atgggggttt tccggtacac gatgagatct gcttcgtcca ggaccttcac
    52621 cgtaagcgct tcattgctca aaaaagggtc cagcttttcc gtggcaacca tcctccccga
    52681 taagcggtcg atatagattt tcaccaggta ttcatggccg ggcatcatgg ggccgaattg
    52741 attgttccgc ggcacaaaca ggtccttcat caggccattg tccaaaaaag cgccgtgggg
    52801 actggtaccg gtcacccgca ggcgcaaaat atcccccacc actcctttgg gttttagggt
    52861 cgtggcaatg ggacggcctt ccttgtcgtg gtaaataaag accttcagtt cgtcccccgt
    52921 cttcgcgcct tccggcacca cccgttttgg gagcaagagg ccttctcccc catcatccag
    52981 gtatacgccg tttgaagctt cccggatgat cttgagcata ttgaattcgc cgacccggat
    53041 catgaggcaa aggtagcagg aaatgatttg ttgaatagct aatgggtcgg tagccggatc
    53101 cggcagttta ataaaaaaca ggctaaaatt atattttgta ttttggcgcc cgtttaaact
    53161 ccaaataaaa tccaaatgaa aaagacctac ttgattatag cctcaatgct gtttctttta
    53221 aacacggtta cccaggccca atcctttacg ccctggacct gggacactta cggcctggcc
    53281 tttaaggcgc cttccaatat gatcgtggaa accaacaacg gggaagaatt tggggctaaa
    53341 ctcaagaatg acctcctgca tttatatatt tttccctgga aagacgcaga actgactacc
    53401 gggagcctga atgccgaggt ggaggcactg gctgaatccc tgggttatga gggatcggaa
    53461 gtggtggaac tggagctcaa ttcttttgaa ggatcctatg tcattggaaa gaaagacgaa
    53521 gtcacgagca tcatcctcgg cctgctggac cccgaaagcg aaaacaattt ttacgtcctc
    53581 atcgtatatg gtccaggtta tgaaaagagg gctctggaga tcgcgaatag tttttcgaaa
    53641 taaacgcctc cccggggctg gccctaatat tttgtaaaac ttatcaattt atcgtaatat
    53701 tgcgatcaaa taaacgatcg tgggcgcagc caaatcagac gaattttcag taaaggacaa
    53761 ccggattgcc tcctatgcca aagccctttc ccatccggcg cgggtggcca tcctcaaact
    53821 gttggttaaa aaaaatgcct gtttttgcgg gtccatcgta gaggagctgc ctctgtcaca
    53881 aagtacggta tcacaacatc ttaaggaact caaggaagcc ggcctgatta aaggcgatat
    53941 cgaggggtcc agggtttgtt attgtattga tgccagggaa tggcagcagg caaagagtct
    54001 tttaggcgac ttgttttcca gttacgaggc gcctccgtcc aattgttgct gatatttttt
    54061 tgaacccatc catcgaaata ttacgataaa aaatataata aacaatgata caaaccgatg
    54121 aacaattaaa agcgctggtg aaacagaaat acggtgaaat cgctttacag gacaaggaga
    54181 ccaatcaatc ctcctgttgc ggttcgggcc cctgttcaac ggggacgtat accattatga
    54241 gcgacgatta ccaggaattg ggaggatata aaccggatgc ggacctgggc ctgggttgtg
    54301 gcctgcccac gcagcatgcc cggatcagga agggggatac cgtgattgac ctgggcagcg
    54361 gcgccggtaa cgattgtttt gtagcccgct cagaaacagg agaagatggc aaggttatag
    54421 gaatcgattt tacaccggct atgatcgaaa aagcaagagc caatgccctc cgggccggat
    54481 attacaatgt agaattcagg ctgggggaca ttgaaaacat gcctttatcg tccaaccggg
    54541 ctgatgtggt catcagcaat tgtgtgctga acctggtgcc caacaaagac gccgtattta
    54601 aagaaatctt ccgtgtatta aaaccgggag ggcatttcag tatttcggat gtggtcctgg
    54661 tcggagaact tcccgaagcg ctaaaacgcg atgccgaatt gtatgcgggt tgtgtgtcgg
    54721 gagccataca gaaagacgca tatctggaat tgataaaatc caatggtttc ggcaatatca
    54781 ccgtgcaaaa ggaaaaaaca atcgttttgc cggatgagat cctgtccgcc tatctcaatg
    54841 aaatggaaat tcgggatttt aaagaggcaa agcagggtat attcagcgtt acggtttatg
    54901 ctgaaaaacc aaaatcgtgt tgcggatctg attgttgcta ggattagtag gattagtagg
    54961 attggtagga ttggtaggat taataggatt ggtaggatta gtaggattgg taggattggt
    55021 aggattggta ggattggtag gattggtagg attggtagga ttagtaggat tagtaggatt
    55081 agtaggatta gtaggttagg tgggatgaag gcatacgatt gggcatctgc ctatggatta
    55141 attccaggga ggtattcctg attgtgcaaa gcatggaagt acccactcgg aatgtcctgg
    55201 tgaaacggct tgttgggtcg aggaaaaaga tgtttaaata aaaccattgt tttgatcctt
    55261 acttctgctc acggcggatt tttataattt tattatagcc tttaccaaaa atacgaaatg
    55321 aacaaacaag cacctctgat atgggcttcc gttttgctgc tgtttgcggt actcactctt
    55381 tttatgagca gttcggtctt gtttgatatg tttggcataa gggagcggga aggaaattat
    55441 gttccttttg tggtctgggc caattttttt gcaagcctgt tatacatggc ttcggtttat
    55501 ggcctggtgc acaggcaaaa atggacggtt cgcgttttgg ggatggccct gatcttgctg
    55561 gtggtcacgt ttaccgcttt tctttttcat atccgcagcg gaggattata cgaaaccaag
    55621 accctaggtg ctctggtttt cagaacaggc gtcacgggtt tatttacttt tattgcgtgg
    55681 aaaacaagcc aacaacctaa aaggacctga cccatgaagt ctgaaaagga aaaaatgctc
    55741 gccggggaaa agtatgatcc gaccgataaa gtgctggcca atgaaaggac ccggacccga
    55801 ctcctccttc tcgaattgaa taattcaagg gaggatgatc cggaaaaccg gatgcgcata
    55861 ttaaaggacc tgatcccgaa ctcgggccct aatctgtggc tacagccccc gttttattgt
    55921 gattacggaa gcaatatgct gatcggggaa agggtgtttt ttaatttcaa ctgcgtggtc
    55981 ctggatgtca tgcaggtgcg tattggaagc cggaccaaat tcgggcccaa tgtccagatt
    56041 tataccgcta cccatcccat tgaccacgaa gaacgcgcat caggacttga atttgccaaa
    56101 tccatttata ttggcgagga cgtatgggtg ggtggttcgg ccgtgatatg tcccggtgtg
    56161 accatcggcg accgtacgat cattggcgcg ggcagtgtgg tgacccggga tatcccatcg
    56221 gatgttttcg ccgcggggaa tccttgcaga gtagttaaac agttagcaag ttagcaagtt
    56281 agcggttgat ggcaatataa aaatgagtca gcaatttttt tataatatgt t
//
LOCUS       DAOQXT010000020        54482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_635_length_54482_cov_5.612545, whole genome shotgun sequence.
ACCESSION   DAOQXT010000020 DAOQXT010000000
VERSION     DAOQXT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 54482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54482
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_635_length_54482_cov_5.612545"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /gene="secF"
                     /locus_tag="PKM27_05235"
     CDS             <1..823
                     /gene="secF"
                     /locus_tag="PKM27_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNR06700.1"
                     /translation="GSPIIKAIDISNTYNVTTDYLINDQEADAPDRVMAKIYDGLKTL
                     PGAELLSLEDFKNPDGKGVHILSFTKVVPTIADDIRTSAIWSTVVGLIVIFLYILVRF
                     SKWQYSLGATLALAHDVLITVSVFSLFHGWFPISLEIDQTFIAALLTLIGYSINDTVI
                     VFDRIREYMSHYTTLSQREVVNKAINSTLTRTVITSMTVFFTVLVLLIFGGSSIKGFA
                     FAMTVGVLAGTYSSIFMAAPVLVDLERVGNKTDKSVAHKTSAAKPAVKPEAAKQK"
     gene            923..2917
                     /locus_tag="PKM27_05240"
     CDS             923..2917
                     /locus_tag="PKM27_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR06701.1"
                     /translation="MKSFFVITALIIAARCTTVQKAKTGADAFDRKQYFLAASLYEKE
                     FGANAERNIRAKLAYGAAMSYQKTNETARSIPWFREAAELGFGDVAWRQYGLALIQNR
                     AYDEAIAVFESRLQEKGNSEEFRLLLSSARQAKALYLDKLDIYEVYPLPVNTEASEYA
                     PVISVRGELLFTSDRPSGTGEENYKWTGRKFSDLFTATEGSSEATLLDPAINTTANEG
                     TACFSQDGNILFFTRCGLSLDQPDAYCKLFVTKRDGAGWSEPQVLAFQKEQVNYMHPC
                     LAASDSVLFFVSDHERGEGGFDIYYSEWKEDDWSEPERLGKRINSPSNEKFPFMFRDT
                     LYFASDRLGGLGGLDIYKSFVNENGDWQPPINLKSPLNSSEDDFALIIDPGSSEDGQV
                     HGFFSSSREGGMGKDDLYRFVRTKADESILIAQEPGKPEDSAALKKPVRYQAYLSIRV
                     VQTIRENPDDPNSRVIGKNPIPNTLVLLREGATPIQLRTNNNGNVLREINYDENYFVL
                     ATAPGLLNASREISTYNERDENNPTKTFNVEITLDRPFTGKEVLIPDIFYDLDKWDIR
                     PDAEPPLNTLVQLLKDNPRIRIQLSSHTDCRGTDEYNLDLSQKRAQAAIEYLIKNGID
                     VNRLLPKGYGETQLINKCICEQCTEDEHQANRRTTFIVLE"
     gene            complement(2966..3382)
                     /locus_tag="PKM27_05245"
     CDS             complement(2966..3382)
                     /locus_tag="PKM27_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06702.1"
                     /translation="MKEFLATVKHYRVPDLVQDWLESPKVVLVHKKPAPDEQAFLEKM
                     TAAFQWKPEEILQVHHPAETAVNYQLLWAKPALKLVLLFGMPPAEIGIHIQARPYQLA
                     PCHPFHLYFVDSLDQVARSREMKAKLWNDLQKIDLG"
     gene            3558..4631
                     /locus_tag="PKM27_05250"
     CDS             3558..4631
                     /locus_tag="PKM27_05250"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNR06703.1"
                     /translation="MSYPIKIEKTTQSRINQVDLNRVAFGRTFTDHMFVADYVNGEWT
                     NLEIKPVSDILIHPANLTLHYGQAIFEGMKAGKDKDGQPVLFRPEMNARRLNFSARRM
                     CMAELPEELFLQALTTLVDLDADWIPENEDGALYIRPYMIAMDSFIGVAASETYRFLI
                     FLMPVGPYYANPVNLWVERKFARAVKGGTGEAKAAGNYAGSLYPARLARQRGFDQLLW
                     TDAIHHKWVEESGTMNVFFIIGDAAVTPSLDGSILHGITRDSVITLLKDKGYEVQERP
                     VSVDELFKAYDKGILREAFGVGTAVVVIPFKSITDEDRVIELDPGSFQIAPMLKSELN
                     AIKKGLIEDKFGWVHHVGVGVGS"
     gene            complement(5031..7757)
                     /locus_tag="PKM27_05255"
     CDS             complement(5031..7757)
                     /locus_tag="PKM27_05255"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNR06704.1"
                     /translation="MKDFSAVFNADPGYIDQLYQQYLSNKETLDDSWKAFFNGFDFAD
                     KADGDLTGKLEKEFKVMALIHGFRDRGHLLSLTNPIRKRRDRHPHLDLADYGLSEQDL
                     DQNFIAGGELNLPGASLRQILDKLRAIYCGSIGFEYNHINNKEKRMWLRDRIEKRAME
                     EDFGLSLDQKKRILQKLNEAVIFEEFLHKKYVGQKRFSLEGGETTIPALDALIESGVE
                     QGVEEVIIGMAHRGRLNILANIVGKTYEQIFTEFEGTAVPDLSFGSGDVKYHQGYSSQ
                     VEVAGGKTVYLRLAPNPSHLEAVNPVVMGMARAKADVLYQSNYDRILSILIHGDAAIA
                     GQGIVYETAQMSQLRGYYVGGTIHFVINNQIGFTTDFDDARTSTYSTSVATSIQAPVF
                     HVNGDDPEAVIFCSELAAEYRQTFNTDVYIDMVCYRKHGHNESDEPKFTQPEMYKIIS
                     THPNPRELYTQKLIARGEVQKDLAELMEKKFWDMLQDRLDMVKQKPLPYTYQEPDLAW
                     KALTKSNDPRDFESSPVTGIEAGLRDQILNHLLNLPNGFQPLQQINRMLKSYRELTAS
                     GKLDWALAELLAYGSLLAEGKNVRISGQDVKRGTFSHRHAVVYDAETNAEYNRLTTLP
                     GASGRFFIYNSLLSEFGVLGFEYGYSLTTPGHLVIWEAQFGDFYNGAQTMVDQFIASA
                     ESKWQRMSGLVLLLPHGYDGQGPEHSNARPERFLQVSADCNWCICNITTPANFFHALR
                     RQLARPFRKPLIVLSPKSLLRDPFCVSSLDELSGQNQFREVIDDPAAEYAKKIKKVFF
                     CTGQIYYELLRRRTEYKREDIALVRIEQLYPLSQSQVQALLEKYKKAKHYWVQEEPAN
                     MGAWSYMNLQLPGVFELISRPASPSVATGYKKVHDAQLVSLLERCFEQEKAG"
     gene            7976..8230
                     /gene="rpsT"
                     /locus_tag="PKM27_05260"
     CDS             7976..8230
                     /gene="rpsT"
                     /locus_tag="PKM27_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNR06705.1"
                     /translation="MANIKGSQKRAELAEVRRTANRFNKKTARTAIARLREITDKKDA
                     ETFMKKVNSLIDRLAKKNTWHKNKAANLKSSLSKHVNSLK"
     gene            8322..9386
                     /locus_tag="PKM27_05265"
     CDS             8322..9386
                     /locus_tag="PKM27_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNR06706.1"
                     /translation="MFFPATISAIYPLTAHAVSIEIAVAAEDQAHFKFKAGQYLTLRI
                     PSLDKSAHRSYSLSAAPYENILRIGVKKVSQGMVSSWLCNQARPGDRLEVMSPQGNFI
                     VPEHREPLCLVAFASGSGITPVLSIIKEELHRHPESRVRLFYGNKNAASAMYTGDLEA
                     LSANHPGRIEINHIYTEQKTGQADTEGRIDRGKLEQWKSYLFDLQKTDYFILCGPGTM
                     VSHLEESLIQYGIPKDKILTELFSAPSQVHKTPEQSAALPELQEGYTIRIRFEKKVLA
                     LKVNSNKEVILDLGLKQKLDLPYSCKSGVCSTCQAKLLSGEVKMDNNYVLNEAELNQG
                     LILTCQSHPLTPVVEVDYDL"
     gene            9496..10383
                     /locus_tag="PKM27_05270"
     CDS             9496..10383
                     /locus_tag="PKM27_05270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNR06707.1"
                     /translation="MAYSDSRYGYGGYTRRTGFRWGGLLMALLLAGYALCKYYSSSQY
                     NEITGEEQHVGAITKEQEIALGLESAPYMAQQHGGLYPDERAQAYVKKVGHRIVQNSV
                     VQKSGYQYDFHLLADPQSVNAFALPGGQVFITLALFSRLENEDQLAGIFGHEIGHVIA
                     RHGAQRMAQQELAQGLTGAAVIAAGDYQSAQGIQMIANLISMSYGRDQELESDDLGVR
                     FMYEAGYRPEALLDVMRILEAASQGQRQPEFFSTHPSPENRLQKIQDAIDKYTRGKPN
                     QISPSEEPIIKLKDPNLQF"
     gene            10442..13648
                     /locus_tag="PKM27_05275"
     CDS             10442..13648
                     /locus_tag="PKM27_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNR06708.1"
                     /translation="MIRSSIQSISWLALTSGLVLGQLFFSCKGEKEDARLPDVVDFNF
                     HIKPILADRCYKCHGPDEKAREANLRLDIRDGIFGKTKKGEKIIAAGNPDRSALIQHI
                     YSTDPERSMPPPESNLSLSELDKAMLRKWISQGAKWKPHWAFLPLEEIKPPKVRSGAE
                     MRNPIDQFIQARLEKEGIKPSPQADPERLLRRIYMDLTGLPPSVETMDEFLKEPSDAK
                     LKDLVEQLFRSDAYAERMAMDWLDLARYADSHGYHADGWRSMYPWRDWVIKAFKENMP
                     FDRFGTWQLAGDLLPEATEEQILATGFNRNHQITAEGGVIDEEYRMEYVFDRVNTTAT
                     AFLGLSMKCAQCHDHKFDPLSQKEYFQFSAFFNNVDELGMNSDDGNCGPTLLLLPDSS
                     KQQLAAIRAQLEKLDSELKAHEDEVLHGESHLKPALNPVKPISTPLEIHLPLDKITKG
                     KNNREIFDSNPKAQVAGQVNLEQGIRGKAACLDDEYEGIFIEDAGLFDRHQPFTAGAW
                     IKPDTGKGFKTIFCNSGDKNSLWRGWDVLIDTGGRILFRLIHALPHDQLKIRTLDKIR
                     FQDWQHVMVTYDGSGRAAGMRVFLNGKRCEASIEYDQLARSILPVDGEFKQVPRPLRM
                     GRWNETFSGEIAFMRGCFDDLRLYSRTLSTLEIANLAGAEKQVHDPETLAEDYLLNRD
                     EKHHQLLNRIRELVSQETRINGGATEVQVMDEDRPRPAYLLERGIYSERREQVSPGTP
                     ASVGSFPTNLPQNRLGLAQWIFDKKNPLTARVTVNRYWQLFFGKGIVATPHDFGNQGA
                     LPSHPELLDWLAGQFIEHQWDLQWLIRTLIESAAYRRASHPREDLKEVDPQNNLLARG
                     PAFRLPAEMIRDNALSASGLLVNKTGGEPVKPYQPDNIWGEKSFFSPKYLVYQQDSGA
                     NLYRRSMYTVIKRTSPHPAMITLDASDRSTCLVQRPSTNTPLQALILLNDPTYIEAAR
                     VLAQRLIKEEGKDLESRIIHAFRSLTGRRPSSQEQKLLEELYETEKERFSKAPEKARQ
                     LISIGESPVDEGLDLVETAALTVVNNTIINHDESYMLR"
     gene            13691..15148
                     /locus_tag="PKM27_05280"
     CDS             13691..15148
                     /locus_tag="PKM27_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNR06709.1"
                     /translation="MHQCNHYRRAHSRREFLAKTALGLGSFAFAGLLDPQSFKANNPF
                     KRTADSLRGLPHFTPRVKRVIYLFQSGAPSQVDLFDHKPQLMKYQGQDLPASIRGTQR
                     QTGMSAGQKIFPVVPSPYSFKASGRSGTLFSEMLPYTAQVADELTIIRSMYTEAINHD
                     PAATFVQTGSQQPGRPSIGSWVSYGLGGENENLPAFIVLLSRNTSSGQPLYDRLWGNG
                     FLPSQHQGVKFRAGKDPVLYLDNPAGMDGTDRRRQLDYLNELQKVQQQQVQDPEIEAK
                     IAQYEMAFRMQTSVPEALDLKGEPDYIFDLYGPDSRKPGTFAANCLLARRLAERNVRF
                     IQLYHQDWDHHGGLPQAMPDVCRATDQPSAALVRDLKQRGLLEDTLVIWGGEFGRTTY
                     SQGKVTAETFGRDHHPGCYSIWMAGGGLKKGFSYGSTDDFCFNVAENGVHIHDFQATL
                     MHLLGIDHEKLNYKYEGRRFRLTDVHGKVVHDILS"
     gene            15154..16482
                     /locus_tag="PKM27_05285"
     CDS             15154..16482
                     /locus_tag="PKM27_05285"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06710.1"
                     /translation="MMDWFGRFHPLLVHLPVGILVLACLAGLVLPKEKINTYLGVFRI
                     VYFSGLFFSILAVLSGLNLAGQGTYDEVNLSQHKWSGISIMALCLLLIWQSRLSGSKT
                     LFRLINGLTLMILTLTGHLGGSMTHGPDYLSVQKDEPEGREKNPVNDSTLILEELVFP
                     VLQARCLRCHQDSARNGGLDLRSVESFLSSNPGDPVIEAGDAVKSELFKRVALPAHHP
                     NFMPPNGLGLSYSEIRLLEWWINLGAPSNKRIADLPESAETDAFLKNQFGLRSSSIDP
                     LAGIRVDPASPAALETLDKLGFAVRPVSSGLHLLDVVPKKSNEALNAEQWNALLALKD
                     QVAWLNLAGTHLNDQSLSYVGQMTNLRKLRLERNQIGDQGIKNLESLVHLEYLNLYGN
                     GISDACINSLAALKKLKELYLGETAITDKGIQTLKEKNPGMEVIGDIELW"
     gene            16687..17055
                     /locus_tag="PKM27_05290"
     CDS             16687..17055
                     /locus_tag="PKM27_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06711.1"
                     /translation="MKRSFWALSSMLFLFLACSKSDDDGDGGGGGGGNNMVDCSTVSK
                     SFATDVSPIFAGTCAAPACHNAGSVNGPGALTNYTSIFANRVAIRAAVASGIMPKNTT
                     LSTNQKNAILCWIDAGAANN"
     gene            complement(17142..17633)
                     /locus_tag="PKM27_05295"
     CDS             complement(17142..17633)
                     /locus_tag="PKM27_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06712.1"
                     /translation="MSPVHKYFLGERLQCSMGIVLALSCIALAFWLRTSQATDFYRGL
                     FYPFVIIPVLLLLICTGVVLRTGRDIKRVDQFISSQREKIISLETPRIEKVMQNFKWI
                     KLIELSILATGIMILVLYRGTGFMGGLGLGLLLQSLIMYGFDLFAEKRGEVYLDILKH
                     LNQ"
     gene            17808..18284
                     /locus_tag="PKM27_05300"
     CDS             17808..18284
                     /locus_tag="PKM27_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNR06713.1"
                     /translation="METETIYQVQEVQWTEKTFVTKRDTVRFDRLSGFFTQAYGEIYT
                     AIQNAGIRPMAEPFAIYYAIDEVRMETDLAAAVPVPEDFMIPAGFHPVTIPASKALYL
                     SYYGGYENMTPAYAALEKHIAEHGYKKQWMLEQYFSDPEVEKDPAKWRTDIYFIVS"
     gene            18491..19414
                     /locus_tag="PKM27_05305"
     CDS             18491..19414
                     /locus_tag="PKM27_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR06714.1"
                     /translation="MDIQVRHLSKTYGHQKAVDDISFDVHPGEILGFLGPNGAGKTTT
                     MKMIAQYLAVETGDILIGGLSVKDQTLKSQIGYLPESNPLYHEMPVIDYLEYVAALQG
                     LSKEKIKERVREMVKVCGLDVEKHKQISELSKGYKQRVGLAQAMIHDPAILILDEPTT
                     GLDPNQIIEIRELIKNLGRHKTVLFSTHILPEVEATCDRILIINKGKIVADGTAGTLR
                     QQAQGHEIHHIRIEDGATQEILSQVRALPSVQKVEVLDPGKNKFEIISKPNMPSNREV
                     FQLCVQKHWVITELIPFETKLEDIFRDLTMN"
     gene            19442..20155
                     /locus_tag="PKM27_05310"
     CDS             19442..20155
                     /locus_tag="PKM27_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR06715.1"
                     /translation="MDKILVIAKKEFGSFFDSLVAYILLVAFLGFSGFFTWIQGSDIF
                     MRREADLDVFFSVARWTLFFFIPALTMKMLAEEKKTGTIEMLLTKDVTDREVVLGKFT
                     GCLMLVGVALLFTLPYYISVAWLGKMDHGATLTGYLGLLLMSAAYIAIGLFASSITNN
                     QIVAFLLALLIGIFFHLLFDFLAAGSSGWISDLFNTLSLSNHYDSISRGVLDTKDLIY
                     FGSITAIGIFLAEYFISKR"
     gene            20163..21647
                     /locus_tag="PKM27_05315"
     CDS             20163..21647
                     /locus_tag="PKM27_05315"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNR06716.1"
                     /translation="MKKNLTTILLIAGILLFVNLLSRRYFIRLDLTRSKEFTLSPSTK
                     NILKNLDDPITISAYFSKDLPANIAKVRQDFKDLLVEYSNRSGGMIHYEFINPNESEE
                     IEQQAMQQGIRPILIDVREKDQSKQQKAYLGALLKMGERTEVLPFIQPGASMEYDLTT
                     SIKKISNLNKQSIGLVQGFGAAALQELAQVSTALSALYNIEPVDLSNPVDPKFQTLLF
                     VRPRDTVSPAALANLDLFLEKGGKLVIALNSVDGDLQNAMGKVISTGVPEWLSTKGIT
                     VNNDFIIDSRCGQVTVQQQQGFFTMMTPVKFPYLPMAADFSEHPVTKGLEQVIFPFAS
                     SISVQADSSLRFTALVHSSEKSNTLPAPAFFDINKQWTEGDFTKSRIPMAGVVEKTNG
                     WKMVVFGDGDFAVNAQEGRAMAPDNINLLVNSVDWLSDDTGLVELRTKGATTRPIKEI
                     DESQRSLIKYANFLIPVLLVILYGFIRSQKMKAKRLARMQERYA"
     gene            21665..22600
                     /locus_tag="PKM27_05320"
     CDS             21665..22600
                     /locus_tag="PKM27_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNR06717.1"
                     /translation="MNNRKLLYVLLALGGLFLLSQLFNRKKERSFKSELVSLDTATVS
                     RIVLHPQADGHHEIQLEKAGGRWFAQQGSLKVPVAPGALNGILNELVSIKVKSIATRS
                     KDRWAEYEIGDSTGSRVEVFSGNKKLADFYSGKFGFNPQGNSMISYIRSAGEDEVYAT
                     DGFQSMTFNATFSSFRDKTLLEIEPEQVTGIRSDFQGLFHSLIKDSTKWTLDDRPGPD
                     SASVANYLQNIQRLNGTAFLDGFSPSGAPSYGFEINTGGSSVNLKVYASPDSLKPFII
                     HSSANPDAFFHEDSTGAFNSLIGGWNDLLKKSIGK"
     gene            22886..26479
                     /gene="smc"
                     /locus_tag="PKM27_05325"
     CDS             22886..26479
                     /gene="smc"
                     /locus_tag="PKM27_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNR06718.1"
                     /translation="MQLNSIEIKGFKSFADNTIIRFNDKVTGVVGPNGSGKSNVVDAI
                     RWVLGEQKPTELRLERMSDIIFNGSKKRKPGGVAQVSLSFDNTKNLLGTEYQQVTISR
                     LLYRSGESEYRINDTPCRLRDIHSLFMDTGIGSNSYAIIALGMVDEILQDKDHSRRKM
                     FEQAAGVTKYKQRKHETMLKLKATSEDLARIEDILFEIENNLKALEKQASRAEKYLDL
                     KAQYKSRSLELARLETQEIYSKNHHLQKILETEMDKLRGIEANHSLAEAGLEESKKAI
                     LDEEHSLSSFQKELNALIQHIQEYENAKRIAQQKIEFYQANREKLARQREEWNERQKQ
                     YDLDIQGKVAQLEQLTLTAAELEKELAGMEAERNAIEGQHGEVKTFFESLQRDQQEIE
                     KDLFEKEKSQAINHNRIDHLQQNINRLSDENRLRDELSATLSVEMENLNRSLEQLSLE
                     IRQEEEEEASRSGRLAEIHKIIEGIRQQKASVERKLDAKRNEHQLLKSMVENLEGYPD
                     SIRFLHKNWKEDLPLLADLLYAPEEYRATIEQFLEPYLSHYIVHSYDEASYAIHMLSD
                     SQKGKANFFILEAITPPDGESMRMEGLIPALDLLKFDGLYSDLFRRIFHNVFIEADQL
                     DKARFAEYDRMNITVLSASGNFVKKQFTLSGGSVGLFEGKKLGRRKNLEILEEELGHL
                     ERELDLVAAELDAQEDQARTLDHTRFLQKLESLKIAFEKKQKEEIQIRTRLENLNELK
                     AHSEASLEQLGKTILEIEILNEELREGIQSKRNVLNELVEKMIHIDSNYQVVALSLSQ
                     AHDKINQKQIENIRHQSRLDNLVQDIEFQKGRMREADQQVLKIAETQEQEESLHDQIT
                     LELERLNESLINLYEERKSKESGLNVFEQEYFKKRNLIHQQEQVLRDLHREQSLSQTL
                     INGLKDQLKELEFRLETVKERLRIEFHIEELPQPEPADPESGEEVDYALPEGASPADL
                     LKEKVRRLRDRIDNYGEINPMAVEAYREMKERYDTIFNQRQDILNAKDSLLNTIQEID
                     GSATTLFLDAFTKVREHFIRVFRSLFTEGDSCDLILLNPDQPLESDIDIIAKPKGKLP
                     KSLSQLSGGEKTLTATALLFSLYLLKPAPFCIFDEVDAPLDDSNIEKFNHIIRDFSSE
                     SQFIIVTHNKQTMASVDIIYGVFMDEPGVSKVTAVDFRDFEHISLLETTAN"
     gene            26679..>28873
                     /locus_tag="PKM27_05330"
     CDS             26679..>28873
                     /locus_tag="PKM27_05330"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HNR06719.1"
                     /translation="MSKKNSRRTFFKQTVAGTTASILSVPALQGMASIAGESGHTTME
                     DAHVYNGAYSGENLNRIAFPIGGIGAGMFCIEGTGAISHLSIRHQPAIFNEPPVFAGL
                     SVKGKPEWSRILEGPVPAWKKFGQRDSGNGSTGATTGLPRYGQAAFTARFPFATINLQ
                     DLQVPFRVELTGWSPFIPGDENNSGMPVGAFEYKISNAGTQTHECLFSFNSAQFLKID
                     KGKNAIRPTSSGFILEEAGTEEKPFKTEFAVFTDEPGTIVDHCWFRGGWWDPLTMAWN
                     AVRDAKAKAVDPVEQGAPGASLYVPFSLAPGKSKTIRLYFAWYCPESDSSYGKMGIRK
                     ENCDPSSGCCNSPSDIGLDKYDQDFNGQFYKPWYSNRFKSIQELISYWKAQYEDLKNK
                     STLFKEAFYASTLPASVIEAVAANLTILKSPTVRRQYDGRLWNFEGCGESWGCCHGSC
                     THVWNYAQAIPHLFPALERSLRHTEFCESQDEKGHQNFRSNLPIQPTTHDFHAAADGQ
                     LGGIMKAYREWRISGDHTWLAKMFPLVKKSLDYCIETWDPERKGTIEEPHHNTYDIEF
                     WGSDGMHNSFYIGALQAIIRMGQALNRDMKDYESLLARARDYMENKLYNGEFFIQQVQ
                     VEGLKAPNPATVQSFGGEYSREALELLQKEGPKYQYGSGCLADGILGIWIARMCGLED
                     ILDKNKIKSHLVAVHKYNLKSDLSTHANPQRPSFALGKEGGLLLCTWPKG"
     assembly_gap    28874..28973
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28974..29174
                     /locus_tag="PKM27_05335"
     CDS             <28974..29174
                     /locus_tag="PKM27_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06720.1"
                     /translation="LRYDAVDKTLYIDPRTGDFTSFLSTEKGFGTVSLKGNVPRLKLY
                     HGAIEVAKVLLKGEEVKLKMEG"
     gene            29309..32110
                     /locus_tag="PKM27_05340"
     CDS             29309..32110
                     /locus_tag="PKM27_05340"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06721.1"
                     /translation="MKNLLLLVFLSFSTLSVSAQGGRIQIKGIVTDSADQALEAGTIV
                     LLKAKDSSLVSFTRTNDQGAFELKNIKPDEPYLLKITYLGYADFQTTVSPGDGQNILD
                     LGRIRMAASSKTLSEVVVQEQHIPVQIKKDTIEFNSAAFRTLPNANVEDMIKKMPGME
                     VDKEGNISAQGKTVTQVLVDGKKFFGKDPKIATRNLPAEAVDKVQVFDKKSDAAEFSG
                     IDDGERETTINLKLKSNARKGTFGSMSAAAGTGGRFDSRASINKFDGQRQLSFIGMGN
                     NLNQQGFSIGDYLSFSGDAQRMVGGGGGGGRMQVSFNTEDQAIPLDFGGTRRGTGNAW
                     AGGLHFNQNFGKNTEINSSYFYNFSKTFINRVTDRENFLPSGNYNDLSRTEQNSDRQT
                     HRLTIQVDQLIDTFSSIRFNGNFSFNDNYQDHQSLTQQRVASETINEVDRYNASESNA
                     LNLQGTLLYRLKFPRRGRFFSTTLSLNTNDNNTDADNRTIRNYLTGSAPILTDSIFQQ
                     NLRIHDRRIYSAGISFTEPVARRRYFEFNASHRSANTNYNQEVYDLLFGKTIDETLSN
                     GYKNTYSYNRAGFNFRIAEKEINVTTGLTLQQSKLNGLQEQGENKRIRKDFLNLLPSA
                     RLEYDLGSGANLSFNYNANVREPSIEQISPIIDNSNPQYVTLGNPDLRPEYNHNLNLN
                     FRKFNMASFTNFFAFARMTLSRNPIIYSQVVDQYLTTTSKPVNLDKNNLSLSGNLNYG
                     FQVKPVKTRINLSTGYNGSRGFNFIQQAENKYNTHSLNGRVNVEYRPGDAFEFVTGVR
                     RAWNNTVYSINRELNQKSHTTTYNADLQAQYKGFTFSTGLNYFVYKGLSDQFNQQVPI
                     WSASFSKTFLKNKRLELKLTANDILKQNIGINRVSQLNYIEDEEVNTLSRYFLMGIHY
                     TLKGTPSMGGGRRMIRMMN"
     gene            complement(32153..32491)
                     /gene="ytxJ"
                     /locus_tag="PKM27_05345"
     CDS             complement(32153..32491)
                     /gene="ytxJ"
                     /locus_tag="PKM27_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HNR06722.1"
                     /translation="MSWIPLTRSDQLDEIDRNSFDKPVLIYKHSTRCSLSNIAFHRIE
                     GLVRDLPTYFLDLIKHRDLSEEIASRYKVYHESPQLLLIRDGECVYEASHMDIHPDAI
                     RDQVSTFPSG"
     gene            32891..38065
                     /locus_tag="PKM27_05350"
     CDS             32891..38065
                     /locus_tag="PKM27_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06723.1"
                     /translation="MHLAYFLWRFLFLLLYRFVLPDCLREHPEFSPFLSEEVLCLADA
                     GLIRYEGGDTLCMGEKTGLFTLEWTDGDPNFLKPRFTVFRINEIEVDNPGTDQAEFIE
                     IAGPADSSLSGMVLVLFNGNAGSPQNAAYRVIDLDRFRTDSCGFFLVRGPSVADPDAS
                     NPYNDTLISVTGGFLQNGPDAVALYKKDAYVNGTGPVLIDLVDAVVYHNAPGDPDSDL
                     LADLGQAQQYVDKQQGSLARSPDISGLFINDSTTSPGQINLEGKPYEYIWLLTRSDSI
                     LVLQKTGDTADHTIKFQISGPGTYQVYGLSVRHGLFTGMDMDLKTIQEIRDTLQNGFC
                     ASLSDPYPITVRPALQFLGTQTTDCSSFDSRGRFQVDIKWLGPALFPMACPDYDTPLE
                     GTRYSSGDTLCFRTSWIPGGDSLCFALIDEDCKDTVRGSVSRFCDFCGILKAPEPLSR
                     QGLCTDEWYTVIPTGGGLEQVRQATGLIFSEYLEGSGNNKCLELFNGTLDTIDLLQEN
                     YVLEIYFNGQEYPLETISLEGRIDPGAVYTICHNLADSIPLSRADQVSGALDFNGDDA
                     LVLKRMDKVADRIGRVGEDPGSQWGSGLTSTQDNTLIRNGLIYIGDSLVRDPFFPEHE
                     WTGYLANDFDDLGKHQYTAVQYVAESYIFYSDETASPGSVLHIGDTYRIRLRAGALDT
                     LYYAAHSLTYGCSSLSLPLVLSASPDNIVCKRKVQVALDPFTCVKTISSDEVVENPGF
                     CKFSIRVSYPFGTNRWAEGNILDRTHAGLQLVYQAVRHDGNSCWGYLQASGPVSSRRT
                     CLQDTMNCYEWYGVTWTQGIQSVPCTKDSMIVVSQSYADSGCASGFLGTWSRSTLFKN
                     GNIQTGACKDIVHIVKPVMAEIDPPESLTIDCDLVRGTGPEALTPEQLIELQGFGIIS
                     STRRIVPQVSGYRIFPVPGPGCVFIPTYSDVTFALCGNSFVIRRQWNIRDWCTGELAT
                     LIQYITIQDKKAPRPALSRPVLMGETDPHDCMGTLSMIRPLRFTDCSALQTWFESEYI
                     DPGQPWKRVHFRSERISDLNLPEGNHVVEFYALDACGFSAKAEVEASIYDRSAPEAVC
                     KSGLTMSSDPATCWARIYAEDLDDGSRDGCKTKLHFAVASMGEMDYWKKYWLGQLKER
                     LGPVRFEVLKDQCFLLTDHWINTWVFRDYANLQKPGTHPLIFRVYEASSIPRLDPHLF
                     NGGSHLWYCYNAYALARLEINWLASSGASPDLFWPEGQFSDSLLQVLEDSIFQAPGPI
                     YEKARPLPVCEGNPKIGASGMNGCPGALYQDCFTAVTLTDKIPPRAEEPEDLWLFNDG
                     AETGRACNKNHEKPSWRTGDCLDPFHKPLGQVVLSQNDQIIKEDPRGHPFGYYGCNPN
                     DRLILDENGRRLVPCDDRGVPVYCPEWLLQDRFDHIGPDAYERYFYRPAYDQASHKVG
                     RTFRISDPCGLDSLAIRSYDTSSIDSCGSGWITRTWTLVDPSGNKTFVDQSLFLRPRS
                     DFEVIFPADTLLFLTPHTPWDSPSLKAPLILDRDVESLVSTYTDEWFIEGKASEGLVL
                     RTWTVKNDCMADFHPHAGPGSEEVLVDDRLVADPLKRPCVYRYLKDGGDGVMVYTQVI
                     RLIQDSKKERGVYFDKTGFPKSPVQWGAGNALLQNHPNPFNRQSVIGISLERQAWGRL
                     MIFSTAGRLLKCIEKEWAAGRHEVWIERNDLGGTGVFYYYFESGFFRAGRKMLIIE"
     gene            complement(38127..38762)
                     /locus_tag="PKM27_05355"
     CDS             complement(38127..38762)
                     /locus_tag="PKM27_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06724.1"
                     /translation="MKKIIGLIGLMVFVTLTLNAQFRAGFKFGVSSVNLAPGSIDVAG
                     VNDFQDFKLAVAKANYGVHAGLFAQIKLAGLFIQPEVMFNSNSADFKLSEITGQPFDV
                     VKRENYQYLDIPLLLGFKLGPLQLGAGPVGHVFLNSGSELFDINGYGQKFKEFKYGYQ
                     ANASLVIGKLYLDVRYEGNSSRFGDHIEFFGNQYSFSKSPSRLIGSIGIAF"
     gene            38865..39491
                     /gene="yihA"
                     /locus_tag="PKM27_05360"
     CDS             38865..39491
                     /gene="yihA"
                     /locus_tag="PKM27_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNR06725.1"
                     /translation="MELKDMRAGKKHKKRWEVEYVGSYPGHEGAPNDGKPLVAIIGRS
                     NVGKSSFINCLCGMRELARTSAKPGKTQTLNYYLVNQAFYLVDMPGYGYAGVSKTTRA
                     NWADWSKNFILNAPMLVCLMILTDSNLPPQDSDVQFINWCGEQQVPLVLIRTKTDKSK
                     DVNINTLEKEFKNKLLETWEELPVMYRHSSITKEGTDALRLAVETMLK"
     gene            39524..41203
                     /locus_tag="PKM27_05365"
     CDS             39524..41203
                     /locus_tag="PKM27_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNR06726.1"
                     /translation="MESPIQPQPHVIEEIEQWPIYRLYEEREKLVEEINQSAIRSLKS
                     KYKGKLDKLLARTIYMEKVRMREDPWNADPQSEPLFWRKIQKSLPESSADPEDIESRS
                     EYLLRKIIERYAEEIAGGFKKNTFLFARRVLHFFFKRLLNAASAKNIFYIFRSRHKIN
                     ERIKLFGPLEKIRTLSTDGVLVIVPTHSSNMDSILIGFALDEIAGLPAFSYGAGLNLY
                     NSELFGYFMNRLGAYRVDRRKKNPVYLESLKAMSNISLQWGTNTLFFPGGTRSRSGSL
                     ETRLKLGLLGTAVEAQREILKYGHSRKIYVVPLVLNYHCVLEARYLIEQHLRSIGKDA
                     YLGSGRDEFKSFKNVFVFLYRLLSKSSEIALSFGEPMDVIGNPVNDQGISLDHKGQPI
                     RLEDYFTSPEEDGEGGEAQRDNVYTSILAEKIVESYHQHNVVFSTNLVSYCAFKTIEH
                     QNPSLDVFALLRLDLDDVVLEPAVLENNILEIQKVLAREEMKGNIRLDQTALLPPSEC
                     IADAINKIGVFHSIKPLKYTKIGKITSGNLKVLYFYHNRLDTYEFLKDFWK"
     gene            41258..41812
                     /locus_tag="PKM27_05370"
     CDS             41258..41812
                     /locus_tag="PKM27_05370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR06727.1"
                     /translation="MNFIVFDLELTCWEGDPAGRMQEIIEIGAYKVDPWRHTKSKFNR
                     FIKPKLYPVLSPFCVQLTTITQAQIDQAKNFKEVIREFMEWAELDIEPHHFIAWGDKD
                     QEYLIENCRLHGLDYDWTKPYTDLKKQYQNVKKLYEPRGLKNATESEGLDFDGTQHRA
                     IDDAFNLCRIFLKYFDEWNLHKGG"
     gene            41995..42825
                     /locus_tag="PKM27_05375"
     CDS             41995..42825
                     /locus_tag="PKM27_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06728.1"
                     /translation="MNKIALFQVLILFLGLIHFPLYAQPGGNTGTLKIKLRLPSDLYF
                     EMAPYRRKQNRIVFNVDALMEGLAIITSDNQTSSIFRQAGMIDTMQKRKTGYLIASPT
                     CDSIVIQDIQDTSIYYFGKGGGEDLANNVHAAEVKIYFSEDADPSIKRVRIKDRVKFG
                     VEANKVRSLKGDPSDLTEPPEHGFIKIEPSGSKKVTLFIDEEPRRGNVLVSIFNANNG
                     RLLATLDPKKQGQRFTTKNKVYVFPLIRPYVISTGTGTKITFQVGDPKVNGSVSASEE
                     "
     gene            42898..43692
                     /locus_tag="PKM27_05380"
     CDS             42898..43692
                     /locus_tag="PKM27_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06729.1"
                     /translation="MFKYILLILPFIYHLSTSVYAQPGVTNVNLQIPDSLYFSRSPGN
                     NKGVRFDADIVLPAIHVFASDNVRSVKLVPSQAQTMGLRRKSFLLAIPLLYTISLQAT
                     TRSYHFGENGGEDKMDNALVAVSYEGKPASPEFNSAHPPVLLKLEDAAILNINHTFPG
                     TGSTASKTYLKVDASNAQTATISINREVRRGKVMVCIFNASNGKLIGMLNPKRNGVES
                     FTARREVWVVPIIKPDRSRPSGSAVDQINFEVGDPVVNGSVRVSEE"
     gene            43808..44644
                     /locus_tag="PKM27_05385"
     CDS             43808..44644
                     /locus_tag="PKM27_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06730.1"
                     /translation="MKTINFSLSVTLLSALVILMMTSQCSRQEVASPPRGHQNLALSI
                     PSGLYYQRLPGDTNALEFTVDSILSGLNILITDNRQNFGMLKSRAQAKRLGRGSGGYA
                     LAIPALYQVSDSNGTIRFAFGQGGAEDSTAAAVVKVIFGSFEETGEKKVKYDPVVIQV
                     ADTQILDYTYNSVPPASTGARQFLHLSASNLNRAYITIDNHPARGRVMVCILDALSGQ
                     FLTLLNPKTDTAQFFATKKSVYIIPIIVPEPSGNFGLAIDSVFFSVGDPVVNGSIRVS
                     EE"
     gene            complement(44692..48303)
                     /locus_tag="PKM27_05390"
     CDS             complement(44692..48303)
                     /locus_tag="PKM27_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR06731.1"
                     /translation="MERIPFYIFRSFPFLFFWTLLPFFISAQAGDFESRLERARGEEK
                     ISILIEGMDSSEAKNEYDKVREYGLQLISVHPEIEKDRKRIKSLKSLSRAYRSLENRD
                     SAIIFCEKYLRGVQNYSDLPEQAWAHNYLGILFYESAQFREALFNFQESLSIKQLLND
                     EDGLSSTYNNLGNLYRDQGDFPKAIEAYEKSLEIKEKLGSPPGRLAVSQYNLGEFYRD
                     RHLDYQALEYYFLALKNYEDSNDSLGIANVLNVLGIMAVKQKDVQQAFEYYSKSKEIA
                     EQIGHHGLLPYLYMNLAEVYQIQGEKQRNIDLLNQGLTMVKESGLPNLIGDFNINLGI
                     SFADQGNYSKALEYLFQADTMYAMVPNVLGQANANVEIGRTLSLDKKYRSALGYLNKG
                     LEVSRQKGDWQIQRAALKNLSEAYHFLNISDKAYQYLKSYNVLNESHDETQETDKLGM
                     LKTLIKLKQDKIKSDLQVIAQRRAKNNIIGFSLLFLLVLLGSYLIYRLQQHQKQIKYE
                     KQKALQLQKIDELKDQFLANTSHELRTPLNGIIGLSDSLIEGACGNLSEQTVDNLRLI
                     SSSGKRLSNLVNDILDFSKARNQDLKLQIQPVDLYVIADLVIKSSEVLIGTKPVRIEN
                     SIARDIPLVKGDENRLQQILFNFVGNAIKFTDQGKITVTAEVKDKQVEIRVEDTGIGI
                     PEDKIDAIFKSFEQLDGSDTRLQGGTGLGLAVTKQLVELHGSTIEVKSSPGKGSTFSF
                     RLPVSQTSRDSSASKSPPAGAALIPAALPRPGDGKELQKKTSETLTRIKEAPATILLV
                     DDEPVNLRVLENHLVLRGFEVDKALNAFDALEKLKQRHFDLVILDVMMPTMSGFELCE
                     KIRERHLANKLPVIMLTAKNTISDLLRGFEVGVNDYLTKPFSIDELMSRIRTHLHLKN
                     FYQASDKFVPIEFLKAIGRESITDAKIGDYTNARFTILFSDIRNYTTLSETLSPEENF
                     NFINAYVGRMGPYIKKYKGFVNQYLGDAIMAIYPDEVEYALDSAIEMQHAIQVYNRER
                     KKKGRDEMEAGMGLHNGALIMGIIGDENRADPTTLADTVNIASRMEGLTKYFGVRVLI
                     SEDVMKQIKDPSRYQFRCLGKILVKGKSKALTMYECLNADPEPVRKLKMDSLWIFDHG
                     ITSFQSGDFIQAVRDFEQVLFSNPSDSVAQYFLKKSMKYQGSGGPKNWDGIEVMAQK"
     gene            48901..51966
                     /locus_tag="PKM27_05395"
     CDS             48901..51966
                     /locus_tag="PKM27_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNR06732.1"
                     /translation="MPILFKGKISNLIRNEINQRIHGELAFSKAGLSLFRSFPDLSVS
                     VYDVVVSGADTFAQTRLFEARSVQVTLDLFKLLRQGLDGVQLKKIHLRDPVLRLVSTP
                     SGTFNYNDLYQTTADSGEAGSKLEIQLSSIKIDQGAFHYYDSAGQVFLSFNHLDHQSS
                     GHYSGTGGSLLSLEHKNVFDSLTIRYGETEYLSELKGLWDGKLSANLDSGSYALSEDH
                     LRLNDLQFDLNLFLHLQEDKTRMDIQVKTPGNDLRQLVSIIPGAYQKNFDKISTSGEF
                     SLEGKLKGYYSPSGSRPEMDIKATINQGMVKYSHLPYPLENINLDLRVKSLDTLFDHL
                     LIQLPKFSFNLQNNPLDGHLTIDHKSSGNQVEGLLRGNMQLADFHQAIPLDSIKLAGN
                     LNLDMQFNFNDQALQNKDFDKIKLAGFAEAEKLDLRYYSYPRLMINRLVATLHPKNVD
                     VRLMAVKFGQSDLEGNATVFHPLAWFSKHQSLTEISIQSSSNTLNISELNYSSSSVTC
                     DTCMSSEAHKIPQLPVLNLNSKASRVIFRDYDLEDIHFQGTYRRDTLYIQSLNGNINQ
                     SRLMVTGRLDNPNAWSSDQAILSGYLGLKTEVFLVDPWMAAGDPQEVTGDTAYSKKLI
                     PRTALSISPEIKLLKYGKYQVRDIRGRLDLIQQTLEVHEGSGKLFNGTVNLDGTFSET
                     GALPIFNFKLDMSRLGFSEMFRVSQTFSKLAPIAEFLEGVFSASMALSGRLDQNQLPV
                     WNELNAAGLVETATAVLSKYKPLEDLAKAIQLPLLDLLKWEKSRNWFEVINGILYLKP
                     FEIRYQGIPISISGSHSLNQEINYNLLFQIPRKLFDKYQVGLVSNQKLDWLIGEVKKK
                     GLDPGRLDTLNINLGLTGPLRNPSSKLQWMADAQHTMKEEVTAAIEATVQEKVDSLEN
                     LAREKAEKVRDSIVETLRKETEEQKARLDSLARSAVDTLTGKVAEKAKEALDSTLKKE
                     SEKILDSLLRQQTDSLLGKKAKEEIDKINEKLKNWDPFRKKPTSEKKNE"
     gene            51959..52582
                     /locus_tag="PKM27_05400"
     CDS             51959..52582
                     /locus_tag="PKM27_05400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNR06733.1"
                     /translation="MNRLTWVPLILAAFLASTACYPPRNAMQKTARKYQSGKLTEDTS
                     YIYQLPFRAGTKSRVIQGYFSRFTHKHRAALDFRMRPGTEIRAARSGIVIRTKDDSNQ
                     GGWNKKYRPDANFVMIEHEDSSRASYRHLKYQGVLVKEGEKVTTGQVLGYSGKTGYSA
                     LPHLHFMVSVFQEGRWVQIPTRFESNPETGYLRPWRKYKSVNVPRYR"
     gene            complement(52591..>54482)
                     /locus_tag="PKM27_05405"
     CDS             complement(52591..>54482)
                     /locus_tag="PKM27_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR06734.1"
                     /translation="VMPAGDNTGAGSPTGIVINEGDALGDKYRGILLSAEAGRNVIYA
                     YKPKRSGAGYRFERFNLIGSVEADNINYKWHEDTLEKSMWFRPSDLCIGTDGAIYIAD
                     WYDPIVGGHQMRERNGYGRIYRIQPKNKSLQSPALRFDTPAGLIEAVRNPAINVRYQA
                     FEKIRALGPSMIPRVQTLLKDPNPYIRMRAIWLLATLGEKGKAEAIRLLDHRDPEKNM
                     VAFRALRQADSSHVPEYAQQLIRKKNPLLNREIAFAVRGMKFADARSLLKDLIGQYQD
                     PDAYMLAAIAAACKRKEDSVYSQILKPLLDSTNWKPAMVAVLHAIRPPGACEDIVHRL
                     SLPGKDSTKIKLLGTLAFIPSPCAVKGMAGLIRSEDSSLVSEARWWLAFRRTNDWQDL
                     VDWGAYDSLIVDPETRIWNEGLNRLMTEKGGARLEAALELALDPSGGLLILQLISNGK
                     LDPALSRQISDTLFYNPNPVVRAMAGDLFWKNKYQYSIDVIKRMEMNEDSGRLVFNRA
                     CIHCHQFGGKGGSVGPDLSAVAGKMDVSSLLDAIINPGAGIVFGYEATAIKTTDGMEY
                     TGFVMSDGDPVRLKDILGKVHDIPQKTIVGRYPLRGSLMPPPQNLMLRDVDLAHLVGY
                     LKNNR"
BASE COUNT        14151 a        14007 c        13402 g        12822 t
ORIGIN      
        1 aggttccccg atcattaagg cgatcgacat atccaatacc tataatgtca ccaccgatta
       61 cctgatcaat gatcaggaag ccgatgctcc ggaccgggtg atggccaaga tctacgatgg
      121 gctgaaaacc cttcccggcg ccgaactcct cagcctggag gattttaaaa atccggacgg
      181 gaaaggcgtg cacattcttt ccttcaccaa ggtggtcccc accatagccg atgatatccg
      241 gaccagcgcc atctggtcca cggtagtggg ccttattgtc atcttcctgt atattttggt
      301 ccggttcagc aaatggcaat acagccttgg cgccaccctc gccctggccc atgacgtgct
      361 catcacggtg agcgtgtttt ccttgtttca cggctggttc ccgatttccc tggaaatcga
      421 ccagaccttt attgcagccc tgctgaccct cattggttat tccatcaacg ataccgtgat
      481 cgtcttcgac cggatccggg aatacatgag ccactacacc acgctgtcac aacgggaagt
      541 ggtcaacaaa gccatcaaca gtaccttgac ccgtacggtg atcacctcta tgaccgtttt
      601 cttcaccgtc ttggtccttt tgatttttgg aggatcctcg atcaagggat ttgcttttgc
      661 catgactgtg ggtgttttgg ccgggaccta ctcatccatc tttatggccg caccggtcct
      721 ggtggatctg gaaagggtag gtaataaaac ggataaatct gttgctcata aaacgtcagc
      781 tgcaaaaccc gccgtaaaac cggaagccgc aaaacaaaaa taaacgaccg tattcaagct
      841 aattcaaggg cttgcttgac tttcaggcaa gctctttgta ttttagtccg ggattgatgc
      901 cccccggatg actaacacat ttttgaaatc cttttttgtt ataaccgcgc tgattattgc
      961 cgcccggtgc acgacggttc aaaaagcaaa gaccggggcc gatgcctttg accgtaaaca
     1021 atattttctc gccgccagcc tttatgaaaa agaattcggg gccaacgccg aaagaaatat
     1081 ccgggcaaaa ctggcttacg gcgccgccat gtcctatcag aagaccaacg aaaccgcccg
     1141 gtcgataccc tggttcaggg aggccgcgga gcttggcttc ggcgatgtgg cctggaggca
     1201 atacggcctg gccctgatcc aaaaccgggc ctacgacgag gccatcgcgg tttttgaatc
     1261 ccggctccag gaaaaaggca attccgaaga attcaggctc cttttatctt ccgcaaggca
     1321 agccaaagcc ttatacctgg acaaattgga tatttatgaa gtctatccgc tgcccgtcaa
     1381 tacggaagcg tcggaatacg ccccggtgat cagtgtgaga ggagagctcc tctttacttc
     1441 tgaccggccc agcgggaccg gtgaagaaaa ctataaatgg accggtagga aattttcaga
     1501 tctgttcacc gccacggaag gcagcagtga agcgaccctg ctggatcccg cgatcaacac
     1561 tactgccaat gaaggcacgg cctgtttcag ccaggacggc aatatcctgt tttttacacg
     1621 ctgcggcctc tccctggacc aacctgacgc ctattgcaaa ctgttcgtga ccaaaaggga
     1681 cggggccggg tggtcggaac cccaggtgct ggccttccag aaagaacagg tgaactatat
     1741 gcatccctgc ctggccgctt ccgattccgt tttgtttttt gtttccgacc atgaaagggg
     1801 ggaggggggc ttcgatattt attattcgga atggaaagag gatgactggt ctgaaccgga
     1861 aaggcttgga aaaaggatca acagcccttc caatgaaaag tttcccttca tgttcaggga
     1921 taccctctat tttgcctcag accgcctggg gggactcgga ggcctggata tttataaatc
     1981 ctttgtcaat gaaaatggtg actggcaacc tcccattaac cttaaatcac ccctcaacag
     2041 cagcgaggac gattttgcat tgatcattga tccggggtct tcggaggatg gccaggtgca
     2101 tggtttcttc agctcgtccc gcgagggggg tatgggcaaa gacgatctgt accgctttgt
     2161 ccggaccaaa gccgacgaat ccatcctgat cgcccaggaa cccggaaaac cggaagacag
     2221 tgccgccttg aaaaaaccgg tccgctatca ggcctacctt tcgattcgtg tggtccagac
     2281 catccgtgaa aacccggatg atcccaacag ccgtgtgatc gggaaaaatc ccatacccaa
     2341 taccctggtc ctgttgcggg aaggggccac cccgatccag ctccgcacca acaacaacgg
     2401 gaatgtgctc cgagaaatca actacgatga aaattatttt gtgctggcca cggccccggg
     2461 gctgctcaac gccagcagag aaatcagcac ctacaatgag cgcgatgaaa acaacccaac
     2521 caaaacgttc aatgtggaaa tcaccctgga caggcccttc accggcaagg aagtcctcat
     2581 cccggatatt ttttatgacc tcgacaaatg ggacattcgc cccgatgccg agcccccgtt
     2641 aaataccctg gtccaactgc tgaaagacaa tccccggatc cggatccaat tgtcctccca
     2701 taccgactgc cggggcacgg atgaatacaa cctggacctt tcccagaaaa gggcgcaggc
     2761 agccatagaa tacctgatta aaaacgggat cgacgtaaac cgtctgctcc caaaaggcta
     2821 tggggaaacc cagctgatca ataagtgcat ttgcgaacaa tgcacggaag acgaacatca
     2881 ggcgaacagg aggacgacat ttattgtcct ggaataagag ggagaaaggc gaagggtgaa
     2941 aggtgaaggg cgaagggaaa acaatttagc ctaaatcgat tttctggaga tcgttccaca
     3001 atttcgcttt catttcccgg ctcctggcaa cctggtccag tgaatccacg aaatacaaat
     3061 gaaaagggtg gcagggggca agctgatagg gccttgcctg gatatgaatt ccgatttccg
     3121 ccggaggcat cccaaacaag agcaccagtt ttaatgcggg tttggcccag agcaattgat
     3181 aattcactgc ggtctcggcg ggatggtgga cctggaggat ctcttcgggt ttccattgga
     3241 atgccgccgt cattttttca aggaaggcct gttcatccgg agccggcttt ttgtggacca
     3301 gtaccacttt cggagattcg agccaatcct ggaccaggtc cgggacccgg tagtgtttga
     3361 cggttgcaag aaactccttc atatgaagcg gattaaggga tgcgggcaaa tccggtggat
     3421 ttacaggtat gttgtcgttg taatagaaaa gtttaagtgg tatactcgca gccaaagggc
     3481 gcgaaacctc ctgccttcct ccggcaaccc attctgaaaa tatttatatt tgcaaagata
     3541 accattcatc cattcctatg tcatatccca ttaaaattga gaagaccacg caatccagaa
     3601 taaaccaggt tgatttaaac cgggtcgctt ttggccgcac ttttaccgat catatgttcg
     3661 tcgcggatta tgtgaatgga gaatggacca accttgaaat caagccggtg tcggatatcc
     3721 tgatccaccc cgccaacctc accctgcatt atggccaggc tatttttgaa gggatgaaag
     3781 cgggaaaaga caaggatggc cagcccgttt tattcaggcc cgagatgaat gcccgccgcc
     3841 tgaacttttc ggcgcggcgg atgtgtatgg cggagcttcc cgaagaactc ttcctccagg
     3901 ccctgacgac cctggtggac ctggatgccg actggattcc ggaaaatgaa gacggcgccc
     3961 tctacatccg gccttacatg atcgccatgg acagttttat tggcgtagcc gcttccgaga
     4021 cctatcgatt cctcattttc ctgatgccgg taggccctta ttatgccaat ccggtcaacc
     4081 tttgggtcga aagaaaattt gcccgtgccg taaaaggggg caccggggag gccaaggctg
     4141 caggcaatta cgccgggtcc ctctacccgg ccaggctggc gcgtcagcgc ggctttgacc
     4201 aactgttgtg gaccgatgcc atccatcaca aatgggtgga agaaagcggt accatgaatg
     4261 tcttttttat catcggggac gcagccgtca ccccttccct ggacggttcc atccttcatg
     4321 gcatcacccg ggattcggtg atcaccttac tcaaagacaa aggatacgaa gtacaggaaa
     4381 ggccggtcag cgtggacgaa ttatttaaag cgtacgacaa aggcatcctc cgggaagctt
     4441 ttggggtggg taccgccgtc gtggtcattc catttaaatc catcaccgat gaagaccgtg
     4501 tgatcgagct ggaccccggc tccttccaga tcgcccccat gctcaaatcc gaactgaatg
     4561 ccatcaaaaa agggctgatc gaggacaaat tcggttgggt tcaccatgtc ggggtgggag
     4621 ttgggagtta gaagctggaa gctaggggag gaactacgac cccctggaac taccaggcca
     4681 aaacgcccta acttctaact actagcttcg agcttctaac ttggaagctg gtagctggaa
     4741 gctggaagct aggggaggaa ccacaaactc cccagaactg cccggccaaa acgccctaac
     4801 ttctaactac tagcttcgag cttctaactt ggaagctggg agctggaagt tggtagctag
     4861 gggaggaact acaaaccccc ggaaatgcca ggccaatacg ccctaacttc tagcttcgag
     4921 cttcttactt ggaagctggg agttggaagc tggaagctag gggaggaact acaaactctc
     4981 ggaactgcca ggctaaaacg ccctaacttc taacttctag cttctagctt ctacccagcc
     5041 ttctcctgct caaaacacct ttccagcaag gataccaact gtgcatcatg gaccttttta
     5101 tatccggtgg cgacggaagg ggaggcggga cgggagatga gttcaaaaac cccgggcagc
     5161 tgcaggttca tatatgacca ggcccccata ttggccggct cttcctgtac ccagtaatgt
     5221 ttggcttttt tatatttttc cagcaaggcc tggacctggc tttgggacag gggatacagt
     5281 tgttcaattc tcaccagggc gatgtcttcc cttttgtatt ccgtccttct ccgaagcaat
     5341 tcataataaa tctggccggt gcagaaaaag acttttttga ttttttttgc gtattcggcc
     5401 gcggggtcgt caatgacttc acggaattgg ttttgcccgg acaattcgtc cagggaggac
     5461 acacaaaagg gatccctgag caatgatttt ggagacagaa cgatcaatgg tttccggaac
     5521 ggacgggcca actggcgccg gagcgcatga aaaaaattgg ccggtgtggt gatattgcaa
     5581 atgcaccagt tgcagtccgc gctgacctgg aggaaacgct ccggtcgggc gttggaatgt
     5641 tcgggtccct ggccgtcgta tccgtgggga agcaaaagga ccagaccgct catcctttgc
     5701 catttggatt cggcggaagc gatgaactgg tccaccatgg tttgggcgcc gttgtagaaa
     5761 tcgccaaact gggcttccca gatcaccagg tggccgggag tggtgaggga atatccgtat
     5821 tcaaaaccca gtaccccgaa ttccgataac agactgttgt atataaaaaa ccgtccgctt
     5881 gcacccggca gggtagtgag ccggttatat tcagcattgg tctcggcatc atagaccacg
     5941 gcatgccggt gggagaaggt cccccttttc acatcctggc cgctgatccg tacatttttt
     6001 ccttcagcca gcaaggaacc gtaagcaagc agttcggcca gcgcccagtc cagtttaccg
     6061 gaggctgtga gttcgcgata ggatttcaac atccggttga tttgctgtaa aggctgaaac
     6121 ccattgggta aattcaacag gtgatttaaa atttggtccc ggagtccggc ttcaatgccg
     6181 gtcaccgggg aggattcaaa atccctgggg tcattggatt tggtcaatgc tttccaggcc
     6241 aagtcaggtt cctggtaggt ataaggcagg ggtttctgtt ttaccatatc cagacggtcc
     6301 tgcagcatat cccagaattt tttttccatg agttcggcaa ggtctttttg cacctcgccc
     6361 cttgcgatta gtttctgggt gtacaattcc ctgggattgg ggtgggtgga gatgatcttg
     6421 tacatttccg gctgggtaaa cttcggttca tccgattcgt tgtgcccgtg ttttcggtaa
     6481 caaaccatgt cgatgtacac gtcggtgttg aaggtctgcc ggtattccgc agccagttcc
     6541 gaacaaaaga tcacggcctc cggatcgtcc ccgttgacat ggaaaaccgg agcctggatg
     6601 gacgtcgcga cactggtgga ataggtggac gtgcgcgcat cgtcgaaatc cgtcgtaaaa
     6661 ccgatctggt tgttgatcac aaaatggatg gttcccccta cataataacc cctgagctgg
     6721 ctcatctggg cagtttcgta gacgatgccc tgcccggcaa tggcggcatc gccgtggatg
     6781 aggatcgaga ggatccggtc ataattgctc tggtaaagca cgtcggcctt cgccctggcc
     6841 atgcccatca ccaccgggtt gacggcttcc agatgggaag gattgggggc cagcctcagg
     6901 tatacggttt taccgccggc gacttccacc tgggaggaat aaccctggtg gtatttgaca
     6961 tctcccgatc caaaactcag gtcgggaacg gcagtgcctt caaattcggt gaaaatctgt
     7021 tcgtaggttt ttccgacaat attggccagg atattgagcc tgccccggtg ggccatgccg
     7081 atgatgacct cttccactcc ttgttccact ccggattcaa tcagggcatc cagggccggg
     7141 atggtcgtct ccccgccttc cagggaaaac cgtttttgcc cgacgtattt tttatggagg
     7201 aattcctcaa aaatcacggc ttcgttgagt ttttgcagga tgcgtttttt ctggtcgaga
     7261 ctgagaccaa aatcctcttc catggccctc ttctcgatcc ggtccctgag ccacatccgt
     7321 ttttccttgt tattgatgtg gttgtattca aagccgatgg acccgcaata gatcgctctc
     7381 agcttatcca ggatttgccg caggcttgct cccgggaggt tcaactcccc gcctgcaata
     7441 aaattctgat ccaggtcctg ctcgctcaac ccataatcag ccaggtccag atgcggatgg
     7501 cggtcccggc gtttgcggat gggattggtg agggacagca ggtggccccg gtccctgaag
     7561 ccgtggatca gcgccatgac cttaaattct ttttccagct tgccggtcag atctccatcc
     7621 gccttgtccg caaaatcaaa gccgttgaaa aaagccttcc agctgtcatc cagggtttct
     7681 ttattggaga ggtattgctg gtaaagttgg tctatatagc cgggatcggc attgaagacc
     7741 gccgaaaaat ctttcatttt gattgagttt tgaaaccctc cgcgggtttg gccggacaaa
     7801 aataagccct atttgaatga agcccggagg tatttatatt attatttatt ttaatttgta
     7861 aagggttatt tattaattgc ataaacatat acaatttttt accatacctt tccttgtctg
     7921 attgatcatt taatggtatc tttgcgcccg catttaaacc atttaattct aatcaatggc
     7981 aaatatcaaa ggttcacaaa aacgggccga actggctgaa gtcaggcgaa cagcaaaccg
     8041 ttttaataaa aaaacagccc gtacagcgat tgcgcggctt cgtgaaatca ccgacaaaaa
     8101 ggatgctgaa acctttatga aaaaggtcaa cagcctgatc gaccggctgg ccaaaaagaa
     8161 tacctggcat aagaacaaag ccgccaacct gaaaagctcc ctgagcaagc atgtaaacag
     8221 cctgaaataa attcgtttta ttttctgaaa aaaatatgcg ctggtgcatt ggagtctgtc
     8281 catgcaccag tttttatttt taacgcgaac tttaccgctc catgtttttt ccggccacca
     8341 tctccgcgat ctatccgctc actgcccatg cggtaagcat tgaaatagca gtcgctgcgg
     8401 aagaccaggc tcattttaag ttcaaggctg gacagtatct gacgctgcgc attccttccc
     8461 tggacaagtc ggcgcaccgt tcttattccc tgagtgcggc gccctatgaa aatatcctga
     8521 ggatcggggt caaaaaggtc agtcagggca tggtctcttc ctggctttgc aatcaggcca
     8581 gaccggggga ccggcttgag gtcatgtccc ctcagggcaa ttttatcgtt ccggaacacc
     8641 gcgaacccct ctgcctggtc gctttcgctt cgggctccgg cattacccct gtcttgtcca
     8701 tcatcaaaga ggaattgcac agacatccgg aaagccgggt ccggttgttc tatggcaata
     8761 aaaatgcagc ctccgccatg tacaccggag acctggaagc cctctcggca aatcacccgg
     8821 gccggatcga aatcaatcat atttataccg aacaaaagac cgggcaggcg gatacggaag
     8881 gacgcattga ccggggcaag ctcgaacaat ggaagtctta tttgtttgac cttcagaaaa
     8941 cggactattt tatcctgtgc ggcccgggaa ccatggtctc ccacctggag gaatccctga
     9001 tacaatatgg gattccgaag gacaagatcc tcacggaatt gttttccgct ccttcccagg
     9061 ttcacaaaac accagaacaa agcgccgccc tccctgaatt acaggaaggg tacacgatcc
     9121 gtatcaggtt tgaaaagaaa gtgcttgcgc tgaaggtcaa ttccaataaa gaggtcatcc
     9181 tcgacctggg cttaaaacaa aaactcgacc tgccctattc ctgtaaaagc ggcgtatgca
     9241 gtacctgcca ggcaaagctc ctctccgggg aagttaaaat ggacaacaat tacgtcctca
     9301 acgaagctga attaaaccag ggactgatcc tcacctgcca aagccatccc ctgaccccgg
     9361 tcgtagaggt ggattatgat ctttagggaa ttcccttcct cccttcctcc cttcctccct
     9421 tcctcctttc gcctttcgcc tttcgccttt cgcctttttc cgaatttgat tacttttaaa
     9481 aactaatcca atgccatggc ttattctgat tcccgatatg gatacggcgg atacacgagg
     9541 cggaccggat tccggtgggg cggccttttg atggcgctcc tgctggccgg ttatgccctg
     9601 tgcaaatatt attcatcctc tcagtataac gagatcaccg gtgaagaaca acatgtgggc
     9661 gccattacca aagagcagga aatcgcttta gggctggaga gtgcgcctta tatggcacag
     9721 cagcacggag gactctatcc cgatgaaaga gcccaggcct atgtcaaaaa ggtggggcac
     9781 cggattgtcc aaaattccgt ggtccaaaaa tcaggctatc aatatgattt tcacctgctg
     9841 gcggatcctc aatcggtcaa tgcttttgcc ttgccgggag gccaggtttt tattaccctg
     9901 gccctcttct ccaggctgga aaatgaagac cagctggccg ggattttcgg gcacgaaatc
     9961 ggccatgtca ttgcgcggca cggtgcgcag cgcatggccc agcaggaact tgcccagggt
    10021 ttgacggggg ccgccgtcat tgcagcagga gattatcaat ccgcacaggg tatccagatg
    10081 atcgccaatc tgatcagcat gagttacggc agggaccagg aactggaatc cgatgacctc
    10141 ggggtgcgtt tcatgtacga agccgggtac cggccggaag ccctgctcga tgtcatgagg
    10201 atcctcgaag ccgcctcgca gggtcagcgt caaccagaat ttttcagtac ccatccgagc
    10261 ccggaaaacc ggctccaaaa aatccaggat gccatcgata aatatacccg gggaaaacca
    10321 aatcagatca gtccgtcgga agaacccatt atcaaactca aggatccaaa tctccagttt
    10381 tgagcggtgt ccatgcaaac ttgctaactt gtcaacttgc taacttgcca actaacaaaa
    10441 aatgatcagg tcttctattc aaagtatatc gtggttggca ttgacctcag gcttggtgct
    10501 cgggcagtta ttcttttcct gcaaggggga gaaagaggat gccaggcttc cggatgtggt
    10561 ggacttcaat tttcatatca aacccattct ggcggatcgt tgttataaat gccacgggcc
    10621 ggatgaaaag gcgagggaag ccaacctgag actggatatc cgggacggga ttttcggaaa
    10681 aacaaaaaag ggtgaaaaaa taatcgccgc cggaaatccg gaccgcagtg cgctcatcca
    10741 gcacatctat tccaccgatc cggaaaggtc gatgccccct ccggaaagca atctgagcct
    10801 gtctgaactg gacaaggcca tgctcaggaa atggatcagt cagggtgcaa aatggaaacc
    10861 gcactgggcc tttttgcccc tggaagaaat aaagcctccc aaagtcaggt ccggagcgga
    10921 aatgcggaat cccattgacc agtttatcca ggcccgactt gaaaaggaag gaataaagcc
    10981 ttcaccccag gcggacccgg aacgcctgct gcggaggatc tacatggatc tcaccggttt
    11041 gcctccttcg gtggaaacga tggatgagtt tttaaaagaa cccagtgatg cgaaactgaa
    11101 ggacctggtg gaacaattgt tccgcagcga tgcctacgcc gagcgtatgg ccatggattg
    11161 gctcgatctg gcccgttatg cggacagcca cgggtaccac gccgatggct ggagatcgat
    11221 gtacccctgg cgggactggg tgatcaaagc ctttaaagag aatatgcctt ttgaccggtt
    11281 tgggacctgg cagcttgccg gcgaccttct gccggaagcc acggaagaac aaatcctggc
    11341 cacgggtttc aaccgcaatc accagatcac ggcggagggt ggggtgatcg atgaagaata
    11401 ccgcatggaa tatgtcttcg accgcgtcaa taccacggct actgctttcc tgggcctcag
    11461 catgaaatgc gcccaatgcc acgatcacaa gtttgatccg ctcagccaaa aagaatattt
    11521 ccaattcagc gccttcttca ataatgtgga cgaattgggt atgaacagcg acgatggcaa
    11581 ttgcggtccc acactgctgc tcctgccgga cagcagcaaa caacaactgg cggccattcg
    11641 cgcccaatta gagaaactgg attcagaact caaagcccat gaggatgaag tcctgcacgg
    11701 ggaatcccat ctcaagcctg ccttgaatcc ggtgaagccc atttccacgc cgctggaaat
    11761 ccatcttccc ctggacaaaa tcaccaaagg caaaaacaac agggaaattt tcgattccaa
    11821 tcccaaagcc caggttgccg gccaggtaaa cctggagcag ggaatccgcg gaaaggcagc
    11881 ttgcctggat gatgaatacg aaggaatctt tattgaagat gccggcctct ttgaccgcca
    11941 ccagccattt accgcagggg cctggatcaa gccggatacc ggcaaaggat ttaaaaccat
    12001 tttctgtaat tccggcgaca aaaattcact ctggcggggc tgggatgttt tgatcgatac
    12061 cggtggccgg attctcttcc gcctgatcca tgccttgcct catgaccagt tgaaaatacg
    12121 gaccctggac aagatccgtt ttcaggactg gcagcatgtg atggtcacct acgatggcag
    12181 cggcagggcg gcaggcatgc gcgtcttcct caacggtaaa cgctgtgagg caagcataga
    12241 atacgaccag ctcgcgaggt ccatcttgcc cgtggatggt gaatttaagc aggtccctcg
    12301 acctttgcgg atgggaagat ggaatgagac cttttccggc gagattgcct ttatgagggg
    12361 ttgttttgat gacctcaggc tgtacagccg gaccctgagc accctggaaa ttgcaaacct
    12421 tgccggcgct gaaaagcagg tccatgatcc ggaaaccctc gcggaggact acctgctgaa
    12481 ccgggatgaa aaacatcatc aattgctgaa ccggattagg gagctggtgt cgcaagaaac
    12541 ccggatcaat ggcggagcca ccgaagtgca ggtcatggat gaagaccgcc ccaggcctgc
    12601 ctatcttctg gagagaggaa tctattccga aagaagggaa caggtctctc ccggcacccc
    12661 ggccagtgtc ggttcttttc caacgaatct tccccaaaac cgtctgggcc tggcccaatg
    12721 gatattcgat aaaaaaaatc ccctgaccgc aagggtgacg gtcaaccggt actggcaact
    12781 cttttttggg aagggtatcg tggctacacc gcacgatttt ggcaaccagg gcgccttgcc
    12841 aagccatccg gaattgctgg actggctggc cggccaattc atcgaacatc aatgggacct
    12901 tcaatggttg atccggaccc tgatcgaatc agcagcctac cggcgcgctt cccatccgag
    12961 ggaagatctg aaagaggtgg atccgcagaa caacctcctg gccaggggcc cggccttccg
    13021 gctcccggcg gaaatgattc gcgacaatgc tctttctgca agcggcctcc tggtgaataa
    13081 gaccggaggg gaaccggtga aaccttatca gcccgacaat atatggggtg aaaaaagttt
    13141 tttctctccg aagtatctgg tctatcaaca ggacagcggc gccaatcttt accggaggtc
    13201 catgtacacc gtgatcaagc gcacctctcc gcatccggcc atgatcaccc tggatgcaag
    13261 cgaccgttcg acttgcctgg tgcagaggcc ttccaccaat acccctttgc aggccctgat
    13321 cctgctcaac gatcccacct atatcgaagc ggcccgcgtg ctggctcagc ggttgataaa
    13381 agaagaaggg aaggacctgg aaagccggat catacatgcg ttcaggagcc tgaccgggcg
    13441 ccgtcccagc agccaggaac aaaaacttct ggaagaactg tacgagacgg agaaggaacg
    13501 gttcagcaag gccccggaaa aggcccgcca gttgatctct attggagaat ccccggtgga
    13561 cgagggcctg gacctggtgg aaaccgctgc cctgaccgta gtcaataata cgatcatcaa
    13621 ccacgacgaa tcctacatgc tgagatgacc ccattgaaac ccattccact ttccgcaaaa
    13681 cccgattgag atgcatcaat gcaaccatta ccgcagggcc cattcccgcc gtgaattcct
    13741 ggccaaaacc gcactggggc tcggctcctt tgcctttgcg ggcctgctgg accctcaaag
    13801 ctttaaagcc aataaccctt tcaagcgcac agcggactcc ctgaggggct tgcctcattt
    13861 cacgccccgg gttaaaaggg tgatctatct ttttcagagc ggcgcccctt cgcaggtgga
    13921 cctttttgat cacaaaccgc aactgatgaa ataccagggg caggaccttc cggcatccat
    13981 ccgtggtaca caaagacaga ccggaatgtc cgccgggcag aaaatatttc cagtggttcc
    14041 ttccccctat tcatttaaag ccagcggccg gagcggaacc ctcttcagtg aaatgttgcc
    14101 ttataccgcc caggtggcgg acgaactcac catcatccgg tcgatgtata ccgaagccat
    14161 caatcacgat ccggcggcga cctttgtaca aaccggctcc caacaacccg ggcggccttc
    14221 cataggatcc tgggtgagtt atggattggg aggggagaat gaaaaccttc cggcctttat
    14281 cgtcctttta tccaggaata cttccagcgg gcaacccctg tatgaccggt tatggggcaa
    14341 cggcttcctt ccttcacaac accagggggt aaaattcagg gcgggcaagg atcccgtgct
    14401 gtacctggac aaccctgccg gcatggatgg caccgaccgg cgcaggcaat tggattacct
    14461 caatgaactt cagaaagtcc agcagcaaca ggtgcaggac ccggagatcg aagccaagat
    14521 cgcccaatat gaaatggctt tccgcatgca gacctccgta cccgaagccc tggatctcaa
    14581 aggcgaaccc gattacatct ttgaccttta tgggccggac agccgcaagc ccgggacctt
    14641 cgcagccaat tgcctgctgg cccggcgcct tgccgaacgc aatgtgcggt tcattcaatt
    14701 gtaccaccag gactgggacc atcatggcgg tctgccccag gccatgcccg acgtatgccg
    14761 ggccactgac cagccctccg cggccctggt ccgtgatctc aaacaaaggg gattactgga
    14821 agataccctg gtgatctggg gaggggaatt cggaaggacg acctattccc agggaaaggt
    14881 gacagcggaa acctttggcc gcgaccatca tcccggttgt tattcgattt ggatggcggg
    14941 aggcggactc aaaaaagggt tttcgtacgg cagcaccgat gatttctgtt ttaatgtggc
    15001 ggaaaacggg gtgcatatcc acgatttcca ggctacgctt atgcatttgc tgggcatcga
    15061 tcacgaaaag ctcaattata aatacgaggg acgccggttc aggctgaccg atgtgcatgg
    15121 caaggtggtt cacgatatat taagctgaaa gtgatgatgg attggtttgg aaggtttcac
    15181 cccctcctgg tgcacctccc ggttggaatc ctggtcctgg cctgtcttgc cggcctcgtt
    15241 ttaccgaagg aaaaaatcaa tacctatttg ggcgtattcc ggatcgttta tttttcagga
    15301 ctgttttttt ccatactggc cgtgctcagc ggcctcaatc tggcagggca gggaacctat
    15361 gatgaagtca atctttcgca gcacaaatgg tctggcatca gcatcatggc cttatgtttg
    15421 cttttgatct ggcaatcgcg gctttccgga tcaaagaccc ttttccgttt gatcaacgga
    15481 cttaccttga tgatccttac cctcaccggt cacctgggcg gcagcatgac ccatggcccg
    15541 gattatttgt cggtgcaaaa ggatgaacct gaaggcagag aaaaaaatcc ggttaacgac
    15601 agcaccttga tcctggaaga actggtcttt ccggtccttc aggccaggtg cctgcgttgt
    15661 caccaggaca gtgcccgcaa tggcggcctt gatctgcggt cggtagaatc tttcctttct
    15721 tcaaatcccg gcgatccggt gattgaggca ggagatgcag taaaaagtga attgttcaaa
    15781 agggtagccc tgcctgctca tcatcccaat ttcatgcctc ccaatggcct gggattatcc
    15841 tattccgaaa tccgtttgct ggaatggtgg ataaaccttg gcgcgccctc gaataaacga
    15901 attgcggacc tgcccgaatc cgctgaaacg gatgcatttt tgaaaaatca atttggtctc
    15961 cggtcttcat ccatcgatcc cctggcaggc atcagggttg accctgcttc tcccgccgcc
    16021 ttggaaaccc tggataaatt gggtttcgca gtaagacccg tttcctcagg gcttcatctc
    16081 ctcgatgtgg ttcctaaaaa atcaaatgag gcccttaatg ccgaacaatg gaatgctctt
    16141 ctcgccttaa aagaccaggt agcctggctc aatctggccg gtacgcatct taatgaccag
    16201 tccttgtctt atgtcgggca aatgaccaat ctccgcaaac tgcgcctgga aagaaatcaa
    16261 atcggtgatc agggaataaa aaacctcgaa agcctcgtcc acctggaata tctcaatctc
    16321 tatggcaacg gaatcagcga tgcctgcata aatagcctgg ccgctttaaa aaagctaaaa
    16381 gaactttacc tcggagaaac cgcaataacg gataaaggga tacaaacatt aaaagaaaaa
    16441 aatccgggca tggaggtcat aggcgatatc gaactgtggt aagttagcaa gttttagcaa
    16501 acacaccgct gatagcttaa ggctgactaa tgactactaa ctattgacta ctgactattg
    16561 actattgact actgactatt gactactgac tactgattac tgaccactga ttactttaaa
    16621 acttttttga tagtccccgc acatcaattc cgccctgcca ttcgtttcta ctactaaatc
    16681 cgattcatga aaagatcatt ctgggccctg agctccatgt tattcctatt ccttgcctgc
    16741 agcaaatccg atgatgacgg agacggcggc ggaggcgggg gaggaaacaa tatggtggat
    16801 tgcagcacgg tatccaagtc ttttgccacc gatgtgagcc ccattttcgc gggcacctgt
    16861 gctgctccgg cctgtcacaa cgcgggcagt gtgaatggcc cgggagccct caccaattat
    16921 acttccatct ttgcaaaccg ggtggcgatc cgagccgcgg tggccagcgg gatcatgcca
    16981 aaaaatacca cgctatccac aaaccagaaa aatgcgatac tttgctggat cgatgccgga
    17041 gccgccaaca actgaggcca atttaaagaa tagagtgggg cagtcatctc agttgaaatt
    17101 gagagacaat aaaataaaaa ccaggcttga atccaagccc ctcattggtt taagtgtttt
    17161 aaaatatcca ggtagacctc accccttttt tccgcaaaga ggtcaaaacc gtacatgatc
    17221 agcgactgaa ggagcagccc aaggccgaga cctcccataa agcctgtccc tcgataaaga
    17281 accagaatca tgattccggt ggccaggatg gaaagctcaa tgagtttgat ccatttaaaa
    17341 ttttgcatca ctttttctat tctcggggtt tccagggaga tgattttttc cctttgggag
    17401 ctgataaatt gatcgacccg tttgatatcc cttcccgtcc gaaggaccac tcctgtgcag
    17461 atcagcagca gaaggacggg aataatgaca aatggataaa acaatcccct gtaaaaatca
    17521 gtcgcctggc tcgtacgaag ccagaaggcc agggcgatgc aggacagggc aaggacgatg
    17581 cccatggagc attggaggcg ctcgccaagg aaatatttgt gaacgggaga cataggtttt
    17641 gcggaatttg atccggaatt aaaattataa aatttcatgg ggtttctttc ccggcccctt
    17701 tgatctgtag agggccaaac cttgaccgta aaatcatcca tccctgattc tcatcatttc
    17761 aaaacctgct ggcggaaatt aaattgaaaa tgcttaaata ttaaaagatg gaaactgaaa
    17821 caatctatca agtacaggaa gtccagtgga ctgaaaaaac ttttgtgacc aaaagggata
    17881 ctgttcgctt tgaccgtctg agcggttttt tcacccaggc ttacggggaa atttataccg
    17941 ccatccagaa tgcgggcatc cgacctatgg ctgagccttt tgccatttat tacgccatcg
    18001 atgaggtcag gatggaaacc gacctggccg ccgcggttcc ggttcctgaa gacttcatga
    18061 tccccgccgg atttcacccg gtcaccatac cggcgtccaa ggcattgtac ctcagctact
    18121 atgggggtta tgaaaatatg acgcctgctt atgccgcact ggaaaaacac attgctgagc
    18181 acggctataa aaaacaatgg atgttggaac aatatttttc cgatccggaa gtggagaagg
    18241 atccggcgaa gtggaggacg gatatttatt ttatagttag ctagttagcc agttagctag
    18301 ttggctagtt agtcagttag caagtttgga ggctgattat attcatatcg gaggcagagt
    18361 ccaactaact aactagccaa ctagctaact gtctaactag tcaactaatt ttaacactcc
    18421 cttcacaaat caccattttg cccggtctgc gttaaaattt taattttgcc gcgcttaaac
    18481 cattcagcat atggatatac aagtgcgtca cctcagcaag acttacgggc atcagaaggc
    18541 ggtggatgat atcagttttg acgtgcaccc aggtgaaatt ctgggcttcc tcgggcccaa
    18601 tggggcgggg aagaccacga ccatgaaaat gatcgcccag tacctcgcgg tcgaaacagg
    18661 agatatcctt atcgggggcc tgtccgtaaa agatcaaacc cttaaaagcc agatcggcta
    18721 ccttccggaa agcaacccgc tctaccatga aatgccggtc atcgactacc tggaatacgt
    18781 agccgccctc cagggcctgt ccaaagaaaa aataaaggaa agggtcaggg aaatggtgaa
    18841 agtttgcggc ctggatgtgg aaaagcacaa acagatcagc gaattgtcca aggggtataa
    18901 acaaagggtg ggcctggccc aggccatgat ccatgacccc gccattctga tcctggacga
    18961 accgaccacc ggcctggacc ccaaccagat catcgaaatc cgcgaactga taaaaaacct
    19021 cggccggcac aaaaccgttc tcttcagcac ccatatcctg cccgaggtgg aagccacctg
    19081 tgaccggatc ctcatcatca acaaaggcaa aatcgtggcc gacggcacgg ccggcaccct
    19141 gcgccagcaa gcccagggcc atgaaatcca ccacatccgc attgaggacg gagccaccca
    19201 ggaaatcctc agccaggtcc gggccctgcc ttcagtccag aaagtggaag tcctcgaccc
    19261 gggcaaaaac aaatttgaga tcatcagcaa acccaatatg ccttccaacc gggaagtatt
    19321 ccagctctgt gtccagaagc attgggtgat caccgaactg atccccttcg aaaccaaact
    19381 ggaggacatc ttccgggacc tcaccatgaa ttaaaaaccc ttaaaagaaa accaacatcc
    19441 tatggataaa atactggtga tcgcgaaaaa agaattcgga tccttttttg attccctcgt
    19501 agcctatatc ctcctggtag cctttctggg attctccggt ttttttacct ggatccaggg
    19561 cagcgatatt tttatgcggc gggaagcgga tcttgacgtt ttcttttccg ttgcccgctg
    19621 gacccttttc ttttttattc cggcccttac catgaaaatg ctggccgaag aaaaaaagac
    19681 gggcaccatt gaaatgctgc tgaccaagga cgtgacagac cgggaagtag tgctcgggaa
    19741 gtttaccggc tgcctgatgc tggttggcgt ggccctcctg tttaccctgc catactatat
    19801 cagcgtggcc tggctgggca aaatggacca cggtgcgacc ctgaccggat acctcggcct
    19861 cctgctgatg tcggcggcgt acatcgcgat cggattgttt gccagcagca ttaccaacaa
    19921 tcagatcgtg gcctttttgc tggccttgct gatcggcatt tttttccatt tgttgtttga
    19981 tttcctggct gcaggcagtt caggctggat cagcgaccta ttcaataccc ttagcctttc
    20041 caaccattac gattccatct cgcgcggggt gctggatacc aaggacctga tctatttcgg
    20101 ttcgatcacg gccattggca ttttcctggc cgaatatttt atttcaaaac gataaacggg
    20161 aaatgaaaaa gaacctgacc accatccttt tgatcgcggg catcctcttg tttgtgaacc
    20221 tgctgtcgag gcgttatttc atacgccttg accttacccg cagcaaagaa tttaccctga
    20281 gcccttccac caaaaacatc ctgaaaaacc tggatgatcc catcaccatc agcgcctatt
    20341 tttccaagga cctgccggct aatatcgcca aggtgaggca ggacttcaaa gacctgctgg
    20401 tagaatacag caaccgctcc gggggcatga tccattacga gttcatcaat cccaacgaaa
    20461 gcgaagaaat cgaacaacag gcgatgcaac aaggcatccg gcccatcctg atcgatgtga
    20521 gggagaagga ccagtcgaaa caacaaaagg cttacctggg agccctgctg aaaatgggcg
    20581 agagaacgga agtactgccc ttcatacaac ccggggcgtc catggaatat gacctgacca
    20641 cctccatcaa aaaaataagc aacctgaaca aacaaagcat cggcctggta caaggttttg
    20701 gtgcggcggc cttgcaggaa ctggcccagg tgagtaccgc gctctccgcc ttgtacaata
    20761 tagaaccggt ggatctttcc aatccggtgg accctaaatt ccagaccctg ttatttgtcc
    20821 ggcccaggga taccgtttcg cccgcagccc tggcaaacct cgacctgttt ctggagaaag
    20881 ggggcaaact ggtgattgcc ctaaacagtg tggacggcga cctgcaaaat gccatgggca
    20941 aggtgatcag caccggcgtg ccggaatggc tgtcaaccaa aggcatcacc gtaaacaacg
    21001 actttatcat cgattcccgt tgcggccagg tcacggtcca gcagcaacag ggctttttca
    21061 ccatgatgac cccggttaaa tttccctatc tgcctatggc ggccgatttc agcgaacatc
    21121 cggtaaccaa gggtctggaa caggtgatct ttccttttgc aagcagcatt tccgtgcagg
    21181 cggactccag cctgaggttc acggccctgg tgcattcttc cgaaaaatca aacaccttgc
    21241 ctgcgcctgc cttttttgat atcaataaac aatggacgga aggcgatttc acgaaaagca
    21301 ggatcccgat ggcgggtgtg gtcgaaaaaa ccaatggttg gaagatggtc gtctttggag
    21361 atggcgattt tgccgtaaat gcccaggaag gccgggccat ggccccggac aacatcaatc
    21421 tgctcgtcaa cagcgtggac tggctgagcg atgataccgg cctggtagag ctcaggacca
    21481 aaggggcgac cacccgtccc atcaaggaaa tcgatgaaag ccagcgctcc ctgattaaat
    21541 atgccaactt cctcattccc gtcctgctgg tgatcttata cggctttatc cgctcgcaga
    21601 aaatgaaggc aaaacgcctc gcgcgaatgc aggaaagata tgcatgacca caacctgatg
    21661 cgatatgaac aatagaaaac tcctttacgt cctcctggcg ctcggagggc tcttccttct
    21721 tagccagttg ttcaaccgga aaaaagaaag gtcttttaaa agcgaactgg tgtcgttgga
    21781 tacggcaacc gtttccagga ttgtcctcca tcctcaggcc gatggccatc atgagatcca
    21841 gctggaaaaa gccgggggcc gctggtttgc ccaacaggga agcttaaagg tcccggtggc
    21901 gcccggtgcc ctgaatggca tcctgaacga actggtttcc atcaaagtca agtccatagc
    21961 cacccgctcc aaggaccgct gggccgaata tgaaatcggc gactccaccg gctccagggt
    22021 ggaggttttc agcggaaata aaaagctggc ggatttttac agcggtaaat tcgggttcaa
    22081 cccccagggc aattccatga taagttatat ccgttctgct ggagaagatg aggtttatgc
    22141 aaccgacgga tttcaaagca tgacttttaa tgccactttt tcctctttcc gggataaaac
    22201 cctgcttgag atcgagcctg aacaggtgac cggcattcgt tctgactttc agggcctttt
    22261 ccattccctg atcaaggatt ccaccaaatg gacgctggat gatcgccccg gccccgactc
    22321 ggccagcgtg gcaaattacc tccaaaacat acagcggctg aatggcacgg ccttcctgga
    22381 cggcttcagc ccttccggag cgcccagcta tggttttgaa atcaataccg gaggctcctc
    22441 tgtcaacctg aaagtttatg cttccccgga tagcctcaaa ccgtttatta tacatagctc
    22501 cgccaatccg gatgcctttt ttcacgagga ttccaccggg gcattcaata gcctgatcgg
    22561 cggatggaat gatttgctga aaaaatccat cgggaaatag taaaacattc gtccggacat
    22621 aaaaaatacg gtgacctgat ccccggaggg atttccctgt ttataaaatc ctggcgagcg
    22681 gcttactgat gatcctgccc gggcgtttta gtggcctcca aataatctta aacggaggag
    22741 gtacagaacc ggcaactttt tagtaaattt agcgaggatc gggtaattct ttatatgtat
    22801 atagttgata tataaaaaga tatagtaata ttcacgggca cacaatgaaa atttttatat
    22861 aaattattta cctatttttg ccgccatgca attgaactcc attgaaataa aaggttttaa
    22921 aagtttcgcg gacaacacca tcatccggtt caacgacaaa gtgaccggag tggtcgggcc
    22981 aaacgggtcc ggcaagtcca atgtggtgga cgccatacgg tgggtgctgg gagagcaaaa
    23041 gcctaccgaa ctgaggctgg aaaggatgag cgacatcatt ttcaatggat ccaagaaaag
    23101 aaagccggga ggggttgccc aggtttcgct cagctttgac aatacaaaaa acctgctggg
    23161 cacggaatac caacaagtca cgatctccag gctcctgtac cgcagcggcg agagcgaata
    23221 ccggatcaac gacacgccct gccgcctccg ggatattcac agcctcttta tggacaccgg
    23281 aatcgggtca aactcttacg cgatcatcgc tttggggatg gtggacgaaa tcctccagga
    23341 caaggaccat tcccgccgga aaatgtttga acaggccgcc ggggtgacca aatacaagca
    23401 gcgtaagcat gagaccatgc tcaaactgaa ggccacctcc gaagaccttg cgcggatcga
    23461 ggatatcctg tttgagattg aaaacaacct caaagccctt gaaaaacaag ccagccgggc
    23521 ggaaaaatac ctggatctga aagctcagta taaaagccgg agcctggaac tggccagact
    23581 cgaaacccag gaaatctatt caaagaacca tcaccttcaa aaaatcctgg aaacggagat
    23641 ggacaaactg agggggattg aagcaaacca cagcctggct gaagccggcc tggaagagag
    23701 caaaaaagcc atcctggatg aagaacactc gctcagttct ttccagaagg aactcaatgc
    23761 gctgatccag catatccagg aatatgaaaa tgccaaacgc attgcccagc aaaaaatcga
    23821 attctaccag gccaaccggg aaaagctggc ccgccagcgc gaagaatgga acgaaaggca
    23881 aaaacaatat gacctggata tccagggcaa agtagcccag ctcgaacaac tgacgctcac
    23941 cgcggccgaa ctcgagaaag agcttgccgg gatggaagcc gaaaggaatg ccatcgaagg
    24001 ccagcatggt gaagtgaaaa ccttttttga atccctgcaa agagaccagc aggaaattga
    24061 aaaagacttg ttcgaaaagg aaaagtccca agccatcaac cacaaccgga tcgatcatct
    24121 tcaacaaaac atcaaccggc tttcggatga aaaccgcttg agggacgaac taagcgccac
    24181 cctttctgtc gaaatggaaa acctcaaccg atccctggag caattgtccc tggaaatccg
    24241 gcaggaggaa gaagaagaag ccagccggtc aggccgcctg gcagagatcc ataaaatcat
    24301 tgaggggatc cggcagcaaa aggcctcggt cgaacgcaaa ctggatgcca agcggaacga
    24361 acaccagttg ctcaagagca tggtggaaaa cctggaagga tatcctgatt ccatccgttt
    24421 tctccataaa aactggaaag aggacctgcc cctcctggca gatcttctat atgcccccga
    24481 agaataccgg gcgaccatcg aacaatttct tgagccttac ctgagccatt atattgtgca
    24541 ttcctatgat gaggccagtt atgccatcca catgttgtcc gattcccaaa aagggaaggc
    24601 caattttttt atattggaag ccatcacccc tccggatggg gaaagcatgc ggatggaagg
    24661 cctgatcccc gctctggacc tgttgaaatt cgacggctta tacagcgacc tgttccgccg
    24721 gattttccac aacgtcttca ttgaagccga tcagttggac aaggcacgtt ttgccgaata
    24781 cgacaggatg aatatcaccg tgctttcggc ttcaggcaat tttgtcaaaa aacaatttac
    24841 cctttcggga ggcagcgtcg gcctgtttga aggtaaaaaa ctagggcgcc ggaaaaacct
    24901 ggagatcctg gaggaagagc tcgggcacct ggagcgggaa ctggacctcg tcgccgctga
    24961 gctggatgcc caggaagacc aggccagaac cctggaccat acccggttcc tccagaaact
    25021 ggaaagcctc aagatcgcct ttgaaaaaaa gcaaaaggag gaaatccaga tccgtacccg
    25081 cctggagaac ctgaatgaat tgaaggccca ttcggaggct tccctcgaac aactgggaaa
    25141 aaccatcctg gaaatcgaaa tcctcaatga agaacttcgg gaaggcattc agtctaaaag
    25201 aaatgtcctg aatgagctgg tggaaaaaat gatccatatc gattccaatt accaggtcgt
    25261 ggccctgtcc ctgtcccagg cccacgataa aatcaatcag aaacagattg aaaacattcg
    25321 ccatcagagc aggctggaca acctggttca ggacatcgaa ttccaaaaag ggagaatgcg
    25381 cgaggccgac cagcaggttc ttaaaattgc cgagacccag gaacaggagg aatccctcca
    25441 cgaccagatc accctcgaac tcgaacggct caatgagtcc ctgatcaacc tgtatgagga
    25501 aagaaaaagc aaagaatccg gcctgaatgt tttcgaacag gaatatttca agaaaaggaa
    25561 cctgatccat cagcaggagc aggtgctgcg tgacctgcac cgcgaacaat ccctttccca
    25621 gaccctgatc aacggactga aagaccagct taaggaactg gaattccggc tggaaaccgt
    25681 aaaagaaaga ttgcggattg aattccatat tgaggaactt ccccaacccg aacccgcaga
    25741 tcctgaatcc ggggaggaag tggattatgc ccttcccgaa ggcgcttcac ccgcggacct
    25801 cctgaaagaa aaagtgagga ggctcaggga ccgcatcgac aactatggcg agatcaaccc
    25861 catggcggtg gaagcttacc gggaaatgaa ggaacgctac gataccatat tcaaccagcg
    25921 tcaggatatc ctcaatgcca aggacagcct gctcaatacc atccaggaga tcgacggaag
    25981 cgcgaccact ctgttcctgg atgcctttac caaagtgagg gaacatttta tccgggtatt
    26041 ccgcagcctg ttcaccgaag gcgacagttg tgacctgatc ctgctcaacc ccgaccagcc
    26101 cctggaatcg gacatcgaca tcattgccaa acccaaaggg aaattgccca aatccctgag
    26161 ccagctttcc gggggtgaga aaaccctcac cgcaacggcc ctgctttttt ctttatacct
    26221 gctcaaaccc gctcctttct gtattttcga tgaggtggat gccccgttgg atgattcaaa
    26281 cattgaaaaa ttcaatcaca tcatccgcga tttttcctcg gaatcccaat tcatcatcgt
    26341 cacccacaac aaacagacga tggcgtccgt agacatcatt tacggcgtgt ttatggatga
    26401 acccggagta agcaaggtca ccgccgtcga tttccgcgat ttcgaacaca tctcgctact
    26461 ggaaacgacc gcgaattgag gtacgaggta cgaggtatga ggtatgaggt atgaggtacg
    26521 aggtaccagg tacgaggtaa gagacctcac taacctctaa cctctgacca ctgacctcta
    26581 acctctgacc actaacctcg tacctcttac ctcgtacctc caacctctaa ccacctacct
    26641 ccaacaattc cttatcttca tattacaaac cgtttgatat gtcgaaaaag aacagccgcc
    26701 ggactttttt caaacaaacc gtagccggca cgaccgcttc catcttatcc gttcccgccc
    26761 tccaaggcat ggcatctatc gccggggagt ccggccacac aacaatggaa gacgcccacg
    26821 tttacaacgg ggcctattcg ggagaaaacc tcaaccggat cgctttcccc atcggaggca
    26881 tcggagccgg aatgttttgc atcgaaggaa ccggagcgat ctcccactta tcgatccgcc
    26941 accagccggc catttttaat gagcccccag tgtttgcggg actttcggtc aaaggaaaac
    27001 ccgaatggtc aagaatcctg gaaggaccgg tgccggcctg gaaaaaattc ggacaaaggg
    27061 attcgggcaa cggatctacg ggtgccacca ccggattgcc gaggtatggg caggcggctt
    27121 ttaccgcccg gtttcctttt gcaaccataa atcttcagga tcttcaagtg cctttcaggg
    27181 ttgaactgac cggttggagt ccttttattc ccggggatga aaacaactcg ggcatgcctg
    27241 ttggggcctt cgaatataaa atatccaatg ccggcacaca gacccatgaa tgcctatttt
    27301 cattcaacag cgcacagttc cttaaaatcg acaaagggaa gaatgccatc cgcccgacct
    27361 cctcgggttt tatcctggaa gaggccggca cggaagaaaa gccgtttaag accgagtttg
    27421 ccgtcttcac cgatgaacca gggaccatcg tggaccattg ctggttccgg ggaggctggt
    27481 gggacccgct caccatggcc tggaacgccg tccgggacgc caaagccaag gcggtcgatc
    27541 cggtggagca gggggcgccc ggggcttcat tatatgtacc cttcagtctg gctcccggaa
    27601 aatcaaaaac catccgcttg tattttgcct ggtattgtcc tgaatccgat tcctcctatg
    27661 gtaaaatggg tatccggaag gaaaactgtg acccttcttc aggctgctgc aattcccctt
    27721 ccgatatcgg cttggataaa tacgaccagg attttaatgg ccaattttat aagccctggt
    27781 acagcaaccg gtttaaatcc attcaggaac tcatttctta ttggaaggcg caatacgaag
    27841 acctgaaaaa caaatcgacc ctttttaaag aggcctttta tgcctccacc ttacctgcct
    27901 ccgtgattga agccgttgct gccaacctca ccatactcaa atctccgacc gtgcgccggc
    27961 aatacgacgg ccgtttatgg aatttcgaag gttgcggaga aagctgggga tgctgccacg
    28021 gttcctgcac ccatgtctgg aattacgccc aggcgattcc ccacctgttt cctgctttgg
    28081 agcgcagcct gaggcatacc gaattctgcg agagccagga tgaaaaagga caccagaatt
    28141 tccgttccaa cctgccgatc cagcctacca cccatgattt tcatgccgcg gccgatggcc
    28201 agttgggagg catcatgaag gcctatcgcg aatggaggat ttcgggagac catacctggc
    28261 tggcgaagat gttccccctg gtgaaaaaaa gcctggatta ttgcattgaa acctgggacc
    28321 ctgaacgcaa aggcaccatt gaagaacccc atcacaatac ctacgatatt gaattctggg
    28381 gttccgacgg catgcacaac agtttttata tcggggccct gcaggccatc atccggatgg
    28441 gccaagccct taaccgggac atgaaggact atgaatcctt attggcccgg gccagggatt
    28501 atatggagaa taaactttac aacggggagt tttttattca gcaggtccag gtggaaggcc
    28561 tgaaagcccc caacccggcc actgtacaat cctttggggg agaatattcc agggaagcat
    28621 tggagctcct gcaaaaagaa gggcccaaat accaatacgg cagcggctgt ctcgccgacg
    28681 gcatcctggg catctggatc gcgcggatgt gcggcctcga ggacatcctc gataaaaata
    28741 agatcaagag ccacctggtc gctgtgcaca aatacaacct caaatccgac ctgagcaccc
    28801 atgccaaccc gcagcgccct tcttttgccc tgggtaaaga gggaggcctg ctcctctgca
    28861 cctggcccaa gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgcgat
    28981 acgatgcggt ggacaagacc ttatatatcg accccaggac gggcgatttc accagcttcc
    29041 tttccacaga aaaagggttt ggcacggtta gcctcaaggg taatgtccca aggttaaaac
    29101 tctaccatgg cgccatagaa gttgccaaag tcctgttgaa aggggaagaa gtgaagttga
    29161 agatggaggg gtgaggattg aggattgaag attgaagatt gaggattgaa ggggggtgac
    29221 attccctttt aacatccctt taatgaatta atatacgaaa acattcgcag tttgaatgga
    29281 gcattcgtta ctttagtggc tgtccctcat gaaaaacctt cttcttttag tttttctttc
    29341 tttttctacc ttatccgtat ctgcccaagg aggccgtatc cagatcaagg gcatcgtaac
    29401 ggattccgcc gaccaggccc tcgaagccgg caccatagtc ctgctgaagg ccaaagacag
    29461 ctcactggtc agctttaccc gaaccaatga ccagggggct ttcgaactta aaaacattaa
    29521 accggacgaa ccttatttat taaaaatcac ctacctgggc tatgccgatt tccaaaccac
    29581 ggtaagcccc ggtgacggac aaaacatctt ggacctgggc aggatccgga tggcggcctc
    29641 atccaaaaca ctcagcgaag tggtggtcca ggaacaacat ataccggtgc aaataaaaaa
    29701 agacaccatc gaattcaaca gcgcggcctt ccgcaccctg cccaatgcca atgtcgagga
    29761 tatgatcaaa aaaatgccgg gcatggaggt ggacaaggaa gggaacatct ccgctcaagg
    29821 gaaaaccgta acccaggtac tggtcgacgg aaaaaaattt tttggaaaag atcccaagat
    29881 cgccacccgg aaccttccgg ctgaggcagt ggataaagtg caggtctttg ataaaaaatc
    29941 ggatgcggcc gaattttctg ggatcgatga cggtgaaagg gaaacgacca tcaacctgaa
    30001 actgaaatcc aatgcccgga aaggcacttt cggctccatg tccgctgcgg ccgggaccgg
    30061 aggccgtttc gattcccggg ccagtatcaa taaatttgac gggcaacggc agctctcgtt
    30121 tatcggcatg ggaaacaacc tcaaccagca gggcttttca atcggtgatt acctgtcttt
    30181 cagcggagat gcccagcgca tggtgggtgg aggcggggga gggggccgca tgcaggtttc
    30241 gtttaacacc gaagaccagg ccatacccct cgatttcggg ggtacacgac gggggaccgg
    30301 gaatgcatgg gcgggaggtc tccatttcaa ccagaacttc gggaaaaata ccgaaatcaa
    30361 ttccagctat ttttacaatt tctccaagac cttcatcaac cgggtcacgg accgggagaa
    30421 cttcctcccg tcagggaatt acaacgacct cagccgtacc gaacaaaaca gcgatcgtca
    30481 aacccaccgg ctgaccattc aggttgacca gttgatcgat accttttctt ccatccggtt
    30541 caacggcaat ttttccttta acgacaatta ccaggatcat caaagcctta cgcagcaacg
    30601 ggtggccagt gaaaccataa atgaggtcga ccggtacaat gccagtgaat ccaatgccct
    30661 caacctccag ggcaccctct tataccggct gaaatttcca cgccggggcc gttttttttc
    30721 aaccacgctt tcgctgaaca ccaatgacaa caatacggat gcggacaacc gaaccatccg
    30781 gaattacctc accggatccg cccccattct tacggattcc atcttccagc agaatttacg
    30841 catccacgac cggcggatct attcggcggg catctcattc accgagccgg tagcccggag
    30901 gcgttatttc gaattcaatg cctcccaccg cagcgccaat accaattaca accaggaagt
    30961 ttatgacctt ctttttggaa aaacgataga tgaaacttta agcaatggat ataaaaacac
    31021 ctactcctac aaccgcgccg gttttaattt caggatagcg gaaaaggaaa tcaacgtgac
    31081 caccgggctg actttgcagc aatcaaagct caacggttta caggaacagg gagaaaataa
    31141 gcggatccgc aaagattttt tgaacctcct acccagtgcg cgcctggaat acgacctggg
    31201 cagcggcgcc aacctgagtt ttaactacaa tgccaatgtc cgggagccca gcattgaaca
    31261 gatctcaccg attatcgaca attcaaatcc ccaatacgtc accctgggga accccgacct
    31321 caggccggaa tacaatcaca acctgaatct caacttccgg aaattcaata tggccagctt
    31381 taccaatttt tttgcattcg cccggatgac cctctcccgg aaccccatca tctactccca
    31441 ggtggtcgat caatacctga ccaccacgag caaaccggtg aacctggaca aaaacaacct
    31501 cagcctttca ggcaacctga actatggctt ccaggtaaaa cctgtaaaaa cccggatcaa
    31561 cctcagtacc gggtacaacg gttcccgggg ttttaatttc atccagcagg cggaaaacaa
    31621 atacaacacc cattccctga acggccgtgt aaatgttgaa taccggccgg gggacgcttt
    31681 cgaattcgtc accggggtgc gcagggcatg gaacaatacg gtctattcca tcaaccggga
    31741 actgaaccag aaatcccaca ccaccactta caatgccgat ctccaggcgc agtataaagg
    31801 gtttaccttc tccaccgggc tgaattattt tgtgtataaa ggcctgagtg accagttcaa
    31861 ccagcaagta cccatatggt cggcaagctt ttcaaaaacc ttcctcaaaa acaaacgcct
    31921 ggaactgaaa ctcaccgcca atgatatcct taaacaaaac atcggcatca accgggtcag
    31981 ccaactcaat tatatcgaag acgaagaagt gaataccctc agccgttatt ttttaatggg
    32041 catccattat accttgaagg ggacgccttc catgggggga gggagaagga tgatccggat
    32101 gatgaattga ccggggaggg attgagcgtc taacaagaaa aaagggcctt ggctatccgc
    32161 tggggaaggt ggaaacctgg tccctgatcg catccggatg aatatccatg tgggaggctt
    32221 cgtaaacaca ttcgccgtcc cggatcagca acaattgtgg agattcgtga tacactttgt
    32281 aacgacttgc aatttcttca ctcaagtccc ggtgtttgat caaatccagg aaataggtgg
    32341 gcaagtcgcg aaccagacct tcaatacgat gaaaggcaat attgctgagc gagcaccgcg
    32401 tgctgtgttt gtaaatcagt acaggcttgt caaaagagtt ccggtcgatc tcatcaagct
    32461 ggtctgaccg cgtgagtggg atccaactca tgaagcaggc ttaaaattcg cttgggcaac
    32521 cgattccccg gttgcaggac tgtataagta ttgccagaag caaaatgccg gcccaaaaaa
    32581 gtttctgccg aagtgcgttt ttcatctcag aatttttaac aaaattaatc attttattcg
    32641 tgctgtatca acgctaaaac cgcctgggtt tgttgcaaaa atacagttaa taattaacaa
    32701 atattattcc atgtaatatg taggtgattg atttattctg aaaatggacg gaattaacag
    32761 atcattgacc tttctatgca cctgggcttt tccctagccc ccacaatcca attcaggcct
    32821 ttacgcgcca tcctttccca agccaaggca atccccttta attttattcc ggacaacacc
    32881 gacacacact atgcacctgg cttatttcct ttggagattc ctgtttttgc tattataccg
    32941 ttttgtactt ccggattgcc tgagagagca cccggaattt tcacccttcc tgtccgaaga
    33001 ggtcctttgc ctggcagatg ccggcctgat ccgttatgaa ggaggagata ccctttgcat
    33061 gggcgaaaag acgggccttt ttaccttgga atggacagat ggggatccca atttcctgaa
    33121 accccgattt accgttttcc ggatcaatga gattgaggtg gacaacccgg gtacggatca
    33181 ggccgaattc attgagatcg caggcccggc ggattcatcg ctgtccggga tggtcctggt
    33241 cctttttaat ggcaatgccg gttcgcctca gaatgcggct taccgggtca tcgatttgga
    33301 ccgtttccgt accgattcct gcggattctt tttggtgcgc ggaccctcgg ttgcagaccc
    33361 ggatgcttcc aatccttaca acgataccct gatctccgtc accggaggtt ttctgcaaaa
    33421 cggtccggat gcggtggctt tgtataaaaa agacgcttat gtcaacggga ccggcccggt
    33481 attgattgat ttggtggatg cagtggttta tcacaacgcg ccgggcgatc cggacagcga
    33541 tttgctcgct gatcttggcc aagcccaaca atatgtggat aagcaacagg gttcccttgc
    33601 cagatcgccg gacatatccg gattatttat caatgattca acgaccagtc ccggacaaat
    33661 aaaccttgag ggtaaaccct atgagtacat ttggttgctt accaggtcgg attcaatcct
    33721 ggttttgcaa aaaacagggg atacggctga ccatactata aaatttcaaa tatcaggccc
    33781 cggtacttac caggtttatg gattatcggt ccggcatggc ctgtttacgg gtatggatat
    33841 ggacttaaaa acgatccagg aaatacggga taccctgcaa aacggatttt gcgcctccct
    33901 ttctgatcct tatccaatca cggtaaggcc tgcactgcaa ttccttggaa cccaaaccac
    33961 cgattgttct tccttcgatt ccaggggtcg tttccaggtc gatataaaat ggctgggtcc
    34021 ggcattgttt cccatggctt gcccggatta cgatactccg ctcgaaggaa caaggtattc
    34081 ctcaggcgat accctttgtt tccgcacgag ctggatcccg ggaggggaca gcctttgttt
    34141 tgccttgatt gacgaagatt gtaaggatac ggtccggggg tcggtaagcc ggttctgtga
    34201 tttttgcggc atcctgaagg ccccggaacc cttatcccgc cagggcttgt gcacggatga
    34261 atggtatacg gtaattccaa ctggcggagg attggaacaa gtcaggcagg cgaccggttt
    34321 gattttttca gaatacctcg aaggcagcgg aaataataaa tgcctggagc tttttaatgg
    34381 caccttggat accattgacc tcctccagga aaactatgtc ctcgaaatct atttcaatgg
    34441 ccaggaatat cctctcgaaa ccatatccct ggaaggacgg atcgatcccg gcgctgttta
    34501 taccatatgt cacaatcttg cggacagcat ccctctcagc cgggcagatc aggtttccgg
    34561 tgcacttgat ttcaacggtg atgacgccct ggtcctcaaa cggatggata aagtggcaga
    34621 tcggatagga agggttgggg aggatccggg aagtcagtgg ggctccggcc tcacttcgac
    34681 ccaggacaat accctgatcc ggaatggatt gatctacatt ggagacagcc tggtcaggga
    34741 tccttttttt ccggagcatg aatggactgg atatttggcc aacgattttg atgaccttgg
    34801 caaacatcaa tacacggcgg tgcaatatgt ggccgaatcg tatatttttt attcggatga
    34861 aacggcctca cccggctctg ttttacatat cggggatact taccggatca ggttgcgcgc
    34921 aggcgccctg gacaccctgt actatgccgc tcacagtttg acctatggat gcagttccct
    34981 gtcgctgccc ctggtattat ccgcttcacc ggacaatatc gtgtgtaaaa gaaaggtaca
    35041 ggtcgctctc gatccgttca cttgtgtcaa aactatttcc tctgatgaag tggtcgagaa
    35101 cccgggcttt tgcaaattct ccatccgcgt ctcttatccg ttcggaacca accgctgggc
    35161 tgaaggaaat atcctggacc gcacccatgc aggtcttcaa ttggtttacc aggctgtccg
    35221 gcacgatggg aacagctgct ggggctatct gcaggcctct ggccctgttt ccagccggcg
    35281 gacctgcctt caggatacca tgaattgtta cgaatggtat ggagtcacat ggactcaggg
    35341 aattcagtca gtcccgtgca cgaaagattc catgatcgtc gtttcccaat cctatgcaga
    35401 ttcaggatgc gcttccggtt ttctgggtac ctggtcccgg agcaccctgt tcaaaaatgg
    35461 caatattcag accggagcct gcaaagacat cgtccacatc gtgaaaccgg tgatggcaga
    35521 gattgaccca ccggaatccc tgaccattga ctgcgacctg gtgcggggta ccggcccgga
    35581 ggctctgacc cctgaacaat taattgaatt acagggattt ggcatcattt catccacccg
    35641 gaggattgtc ccccaagtat ccggataccg gatttttccg gtgccaggtc ccggttgcgt
    35701 ttttattccg acttattccg atgtaacctt cgcgctatgc ggcaacagtt ttgtaatacg
    35761 gaggcaatgg aacatccggg actggtgcac gggagagctt gccaccctga tccagtacat
    35821 caccatccag gataaaaaag cgccgcggcc ggcgctgagc aggccggtat tgatgggaga
    35881 gacggatccg cacgattgta tgggcaccct ttccatgatc cggcctttgc ggttcaccga
    35941 ttgttctgct ctgcagacct ggtttgaatc tgaatacatc gatccgggtc aaccttggaa
    36001 acgggtgcat ttccggtctg aaaggatcag tgaccttaac cttcctgaag gaaaccatgt
    36061 cgtggaattt tatgcccttg atgcctgcgg cttcagcgca aaagcggagg tggaggccag
    36121 catctatgac cggtctgcac ctgaagccgt ttgcaagtcc ggtttgacca tgagttcgga
    36181 tccggcgacg tgctgggcaa ggatttatgc ggaagatctg gatgacggat cgcgcgacgg
    36241 ttgtaaaacc aaacttcatt ttgctgtcgc ttccatggga gagatggact attggaaaaa
    36301 atattggctt gggcaattaa aagaaaggct tggacctgtg aggtttgagg tccttaaaga
    36361 ccagtgcttt ttgttgaccg atcattggat caacacctgg gttttcaggg attatgcaaa
    36421 cctgcaaaaa cccggtaccc accccttgat cttccgggtt tatgaagcct cctccatccc
    36481 gcggctggat cctcatcttt ttaatggcgg gtcccatctc tggtattgtt acaacgctta
    36541 cgcacttgcc cggctggaaa tcaattggct ggcttcgtcc ggtgcttcgc cggatctgtt
    36601 ctggcccgag ggtcaatttt cggacagcct gttgcaggtg ctggaggaca gcatttttca
    36661 ggcaccggga cccatttatg aaaaggccag gcccttgccc gtatgtgaag gcaatcccaa
    36721 gatcggggca agcgggatga acggttgccc gggcgccttg taccaggatt gtttcacagc
    36781 cgtcaccttg acggataaaa taccgccccg ggcagaggaa cccgaagatc tttggctgtt
    36841 caacgacgga gccgagaccg gtcgggcatg taataagaat cacgaaaaac caagctggag
    36901 aacaggggat tgcctggacc cattccataa accccttggc caagtggtct tatcacaaaa
    36961 tgatcaaata ataaaagaag acccaagagg acatcctttt ggttattacg gctgcaatcc
    37021 caatgaccgg ctgatcctcg atgagaatgg ccggaggctg gtaccctgtg atgaccgggg
    37081 ggtaccggtt tattgtcccg aatggttgtt acaggaccgg ttcgaccaca tagggccgga
    37141 tgcttatgaa aggtattttt accgacctgc atatgatcaa gcctctcaca aagtcggccg
    37201 gactttccgg atttccgatc cttgcggcct ggactccctg gcaataagga gctatgacac
    37261 ttcaagcatt gactcctgcg gcagtggttg gatcacaaga acctggaccc tggtggaccc
    37321 aagcggtaat aaaaccttcg tggatcaatc tctgttcctc agaccaagaa gtgattttga
    37381 agtgattttt cctgcggata ccttactctt cctgactccg catactccat gggatagtcc
    37441 ttccttaaag gcgccgctta tcctggatcg ggatgttgaa tccctggttt ctacctatac
    37501 cgatgaatgg ttcatagagg gcaaggcctc ggaaggtttg gtcctgcgta cctggactgt
    37561 taaaaatgat tgcatggcgg atttccatcc tcacgccggg cccggttctg aagaagttct
    37621 cgtcgacgat cgcctggtgg ctgatcccct gaaaagaccc tgcgtgtacc gttatctgaa
    37681 agacggggga gacggggtaa tggtctatac ccaggtcata cggttaatac aagattcgaa
    37741 gaaagaacga ggagtgtatt ttgataaaac aggattccca aaatctccgg tccaatgggg
    37801 agcaggaaat gcccttttac aaaaccatcc caatcctttc aaccggcaat cggtgatcgg
    37861 gatcagcctg gaacgccaag cctggggaag attaatgatc ttttcgactg ccggccgtct
    37921 attaaaatgc attgaaaagg aatgggccgc cgggcgccac gaagtctgga ttgaacggaa
    37981 cgaccttggg ggaaccggag tattttatta ttattttgaa tccggatttt tcagggcagg
    38041 caggaaaatg ctgatcatcg aataaaaaaa aggcccggtg cagaccaccg gacctctatt
    38101 tagtcaattc aattcgatta tcggcgctaa aatgcgatcc caatgctccc gatcaggcgg
    38161 cttggggatt tggagaaaga atattgatta ccaaaaaatt cgatatggtc tccaaagcgg
    38221 gacgaattgc cttcataccg gacgtcgagg tatagttttc caatgaccag ggaagcattg
    38281 gcctggtagc cgtatttaaa ctctttaaat ttctgaccgt agccattgat gtcaaacaat
    38341 tcggaaccgc tgtttaaaaa aacatgtccg accggcccgg cgcccaactg aaggggcccc
    38401 agtttgaaac ccaacaacaa gggaatatcg aggtattgat agttctccct cttgaccaca
    38461 tcaaaaggct ggcccgtgat ttcactgagt ttaaaatcgg cactgttgga attgaacatc
    38521 acttccggtt gtataaagag gccggcgagt ttgatctggg caaacaaacc tgcatggacc
    38581 ccgtaatttg cctttgcgac tgcgagttta aagtcctgaa aatcgtttac cccggccacg
    38641 tcaatggatc cgggagccag gttcacgctg gatacaccaa atttaaatcc ggcacgaaat
    38701 tgtgcgttca gggtaagggt cacgaagacc atgagaccga ttaatccgat tattttcttc
    38761 atcttggcag agatttatga gaggataacg acaaaatcgt gccaactata ttaaataaat
    38821 ttgtaatgtg acgaccatta accccaattt aatgggaacc gttaatggaa ttaaaggata
    38881 tgcgcgcagg gaagaaacat aaaaaacgct gggaagtgga gtacgtgggg agttacccgg
    38941 ggcacgaagg ggccccgaat gacgggaagc ccctggttgc gatcatcggg cggtccaatg
    39001 tgggaaaatc cagcttcatc aactgcctct gcgggatgcg ggaacttgcc cggacctccg
    39061 ctaaacccgg caagacacaa accctcaatt attacctggt gaaccaggcc ttctacctgg
    39121 tcgatatgcc cggttatggt tatgcgggcg tttccaaaac cacccgggcg aattgggcgg
    39181 attggtccaa aaatttcatc ctgaatgcac ccatgctggt ctgcctcatg atccttacgg
    39241 attccaatct gccgccccag gattccgatg tgcaattcat caattggtgc ggggaacagc
    39301 aagtgcccct ggttctcatc cggactaaaa cggataagtc taaagatgta aatatcaata
    39361 ctttagagaa agagtttaaa aataaattgt tggaaacctg ggaagaatta cccgtgatgt
    39421 accggcattc ttccatcacc aaggaaggca cggatgcgct ccgcctggcc gtcgaaacga
    39481 tgttaaaata agcccgaaat ttgaagtagc tttgcagggt taaatggaat cgccgataca
    39541 gcctcagccg catgtcattg aagaaataga acagtggccg atctaccggc tttatgagga
    39601 acgcgaaaag ctggtggagg agatcaacca gagtgccatc cgcagcctga aatccaaata
    39661 caaaggaaaa ctggacaaat tgctggcccg gaccatttac atggaaaagg tccggatgag
    39721 ggaagacccc tggaatgcgg atcctcaaag tgagcctctg ttctggagaa aaattcagaa
    39781 atcccttccg gaaagttcag cggacccgga agacatcgag agccgcagtg aataccttct
    39841 tcgcaaaatc atcgagcggt atgcggaaga aattgcgggg gggtttaaga aaaatacttt
    39901 tttgtttgcc cgtagagtct tgcatttctt tttcaagcgg ctgctgaatg cggccagcgc
    39961 caagaacatc ttttacattt tcaggtccag gcataaaatc aatgaaagga tcaaattgtt
    40021 tggccccctg gaaaagatca ggacgctgtc gacggatggc gtattggtca tcgtaccgac
    40081 gcattcctcc aatatggaca gcatccttat cggttttgcc ctcgacgaga ttgccggcct
    40141 tcccgctttt tcctatggcg cagggctcaa cctttacaat tccgaattgt tcgggtattt
    40201 tatgaaccgg cttggcgcct accgggtcga ccgaaggaaa aaaaatccgg tctaccttga
    40261 aagcctgaaa gcgatgtcca acatatccct tcaatggggt accaatacct tgtttttccc
    40321 gggcggcacc cgttcccggt ccggttcgct ggagacccgc ctgaagctcg gactgctggg
    40381 cactgcggtc gaagctcagc gtgaaatttt aaaatacggg catagccgga aaatttatgt
    40441 agttccgctg gtgctcaatt atcattgtgt gctggaagcc cggtacctga tcgagcaaca
    40501 tctgcggtcc ataggtaaag acgcctacct gggcagcggc cgcgacgaat tcaagagttt
    40561 taagaatgtt tttgtattcc tttaccgctt gttgtccaaa agttccgaaa tcgccctttc
    40621 gtttggtgaa cccatggatg tgatcggaaa cccggtgaat gaccagggca tcagcctgga
    40681 ccataaaggc cagccgattc gcctggaaga ctatttcacc tcaccggaag aagatggcga
    40741 gggaggggaa gcccagcgtg acaatgttta tacttccatc ctggctgaga aaatcgtgga
    40801 aagttatcac cagcacaatg tggtgttcag caccaacctg gtttcttatt gcgcgtttaa
    40861 aaccattgaa catcaaaatc ccagcctcga tgtatttgcg cttttgagac tggacctgga
    40921 tgatgtggtt ttggaacctg ctgtgctgga aaacaatatc ctggaaattc agaaggtcct
    40981 ggccagggaa gaaatgaaag gcaatatccg gttggaccaa accgccttac tgcctccttc
    41041 ggaatgcata gcagacgcca ttaacaagat cggggtcttc cattccataa aacctttaaa
    41101 atacactaaa atcggaaaga tcacttcggg caatctgaaa gtgctctatt tttaccacaa
    41161 ccggctggac acttacgaat tccttaaaga tttctggaaa taaagcgtcc ccggaggtac
    41221 ctgcaccccg gaatgagcgg gatgaaatgg atggaagatg aattttattg tttttgacct
    41281 tgagcttacc tgctgggaag gcgaccctgc cggccggatg caggaaatca tcgaaatcgg
    41341 cgcttataaa gtggacccct ggagacatac caaaagcaag tttaaccgat tcatcaagcc
    41401 gaaactctac ccggtccttt ctcccttctg cgtacaactc accaccatca cgcaggccca
    41461 gattgaccag gcaaaaaact tcaaagaagt gatccgggag tttatggaat gggccgaatt
    41521 ggacatcgag ccccatcatt tcatcgcctg gggggacaag gaccaggaat acctgatcga
    41581 aaattgcagg ctccatggat tggactacga ttggaccaaa ccctatacgg acctgaaaaa
    41641 acaataccaa aacgtcaaaa aactttatga accccgcggg ctcaaaaacg ccaccgaatc
    41701 cgaagggctt gattttgatg ggacccagca ccgcgccatc gacgatgcct ttaatctatg
    41761 caggatcttc ctcaaatatt ttgatgaatg gaacctgcac aagggagggt aggggttacc
    41821 ccagggcagt tttgacataa atcctgcatt cataagagcg taaaaaacag ccaggatccg
    41881 gattgtcctg cggggatccg gcattctaat aaatgatttt ttagggacag cagccgttcc
    41941 cattttcaaa taaatcacgg actatttgta tattcaaaat tatattaccc gactatgaac
    42001 aaaattgcac tcttccaggt cttgatcctg ttcctgggct tgatccattt ccccctttat
    42061 gctcagcccg gaggaaatac cggcactttg aaaatcaaat taagacttcc ttcagatctg
    42121 tatttcgaga tggcccctta ccggagaaaa cagaaccgga tcgtttttaa tgtggatgcc
    42181 cttatggaag gcctggccat catcaccagc gacaaccaaa cttcttccat ctttcgccaa
    42241 gccgggatga ttgacacgat gcaaaagagg aagaccggat atttgattgc atcgccgacc
    42301 tgcgattcaa tagttattca ggatattcag gatacttcca tttattattt tggcaagggt
    42361 gggggagaag atctcgcaaa caacgttcat gcagcagagg taaagatata cttttcggag
    42421 gatgccgatc cttccataaa acgggttcga ataaaggacc gcgttaagtt tggagtggag
    42481 gcaaataaag tccgaagttt aaaaggggac ccttcggatt taacggaacc accggagcat
    42541 ggatttataa agattgagcc ctccggatca aagaaggtga ccttatttat tgatgaggag
    42601 ccccggcggg gaaatgtcct ggtgagcata ttcaatgcaa ataatggcag attgctggcc
    42661 accctggatc ctaaaaaaca aggccagagg tttaccacaa agaacaaggt ctatgtattt
    42721 cccctgatca ggccctatgt catttcgaca gggactggga ccaagatcac ctttcaggtc
    42781 ggcgatccca aggtaaacgg ctcggtatcc gcttctgagg aatgaacagg ggtcattcaa
    42841 agctataccg ggccggcacc atgctccatg ttattcagca aaagaaaaat atcttccatg
    42901 tttaagtata tcttattgat tctgcctttc atttaccatt tatccacttc cgtatatgcc
    42961 cagcctggcg tcaccaatgt aaatctgcaa ataccggaca gtctatattt cagcagaagc
    43021 cccggcaaca ataagggtgt gcgttttgat gccgatatcg tacttcccgc gatccatgtc
    43081 ttcgcctcgg acaatgtccg gagcgtgaaa ctcgtaccat cccaggccca gaccatggga
    43141 ttgagacgca aatcattttt actggccatc cccttgttat ataccataag tttgcaagcc
    43201 accacgcggt cctaccattt cggggaaaac ggcggggagg acaaaatgga caatgccctg
    43261 gtcgcggtat cttatgaagg caagccggca agcccggaat tcaattccgc ccatcccccg
    43321 gtattgctca aattggaaga tgctgctatc ttaaacatca accacacctt tcccggaacg
    43381 gggtcgaccg caagcaaaac ataccttaaa gtagatgcat cgaatgccca aacagccacc
    43441 atttccataa accgggaagt ccggagagga aaagtcatgg tgtgcatatt taatgccagc
    43501 aacgggaaat taatcggcat gcttaatcca aaaaggaatg gggtggaatc ctttactgcc
    43561 aggcgggaag tttgggtcgt ccccatcatc aaacctgatc ggtccaggcc ctccggatcg
    43621 gcggtggatc aaattaattt tgaagtcggc gacccggtcg tcaacggttc ggtcagggtg
    43681 tccgaggaat aggaattccc gggcaaaagg cccaggccga tgccccttgc atttctttat
    43741 gtcgggaatc ttttttatct tccatacgac ggcaatggct gcaatccggc caaataaaac
    43801 aacggctatg aaaacgataa atttttctct gtcagttacc cttctttctg ccctggtgat
    43861 tcttatgatg accagccaat gttcccggca agaggtagct tctcctccgc gcggtcacca
    43921 aaacctcgcc ttgtccattc ccagtggcct ttattatcag aggctgcctg gggataccaa
    43981 tgccctggaa ttcaccgtgg actccatcct ttccggactc aatattctca ttacagataa
    44041 tcgacagaat ttcggaatgt tgaagagccg ggcgcaggcc aaacgcctcg gaagagggag
    44101 tggaggttat gcactcgcca tcccggcctt gtaccaggtt tccgacagca acggcaccat
    44161 ccgttttgct tttgggcagg gtggggcaga ggacagtacc gctgccgctg tggtcaaggt
    44221 catttttggc agttttgagg aaaccggaga aaaaaaggta aagtacgatc ctgtagtcat
    44281 ccaggttgca gatacccaga tcctggacta tacctacaac tccgtccccc ctgcaagcac
    44341 aggggcgaga caatttttgc acctttcagc ctccaatctc aaccgggctt atatcaccat
    44401 tgacaatcat ccggcccgag gccgggtcat ggtttgtatt ctcgatgcct tgtccggcca
    44461 gttccttacc cttctgaatc ccaagaccga tactgcccag ttttttgcaa ccaaaaaaag
    44521 tgtttatatc ataccgatta ttgtccccga accttcaggc aattttggcc tggccatcga
    44581 ttccgtcttc ttttcggtag gcgatccggt agtcaatgga tcgatcaggg tatcggagga
    44641 ataggctaag gatttttcat agcaacgatt cagagggtca gaaccttagg gtcatttctg
    44701 cgccatcacc tcaatcccat cccagttttt ggggccaccg ctgccctggt acttcatcga
    44761 ttttttgagg aaatactggg cgaccgaatc cgaggggttg gagaacagca cctgctcaaa
    44821 atcacggacc gcctgaataa aatcaccgga ctgaaaactg gtgattccgt ggtcaaagat
    44881 ccacaacgaa tccattttga gtttccgtac cggttccgga tcggcattca ggcattcgta
    44941 catagtgagc gccttggatt tcccttttac cagaattttt cccaggcagc ggaattggta
    45001 tctggaaggg tctttgatct gtttcatcac atcctcgctg atcaggacac ggaccccgaa
    45061 atatttggtg aggccttcca tacgggaggc aatgttcacc gtgtcggcca gggtagtggg
    45121 gtccgcccgg ttttcatcgc cgataattcc catgatgagg gcgccgttat gcaggcccat
    45181 ccccgcctcc atttcatccc ttcctttttt cttcctttcc cggttgtaaa cctgaatggc
    45241 atgctgcatt tcaatggccg aatccagagc gtattccact tcgtcgggat agatggccat
    45301 gatcgcatcg cccaggtatt ggttgacaaa acccttgtat ttttttatat agggccccat
    45361 gcggcctaca taagcattga taaagttgaa attttcttca gggctaagcg tctcggacag
    45421 ggtggtataa ttgcggatat cggaaaacag aatggtaaaa cgggcgttgg tgtaatcccc
    45481 gattttggca tccgtgatgg actcccgtcc aatggctttt agaaattcga tggggacaaa
    45541 tttgtccgaa gcctggtaaa agtttttaag gtggagatgg gtacggatcc gggacatcaa
    45601 ctcgtcaatg gaaaagggtt tggtgagata atcgttgacc ccgacctcga agccccggag
    45661 caggtctgag atggtatttt ttgctgtgag catgatcacc ggcagtttgt tcgccagatg
    45721 cctttccctg attttttcac aaagctcgaa tccggacatg gtaggcatca tgacatccag
    45781 gatcaccagg tcaaaatgcc gctgtttcag tttttcaagg gcgtcaaagg cattcagggc
    45841 cttgtccact tcaaaaccac gcaggaccag gtgattctcc aatacccgga gattcacggg
    45901 ttcgtcatcc accaacagga tggtcgcagg agcctcttta atccgggtaa gtgtttcact
    45961 cgttttcttt tgcagttcct tgccatcgcc gggccggggc aaggccgcag gaatgagggc
    46021 tgcgccggcg ggtggtgact tgcttgcgga cgaatcccgg ctggtctggc tgaccggaag
    46081 acggaaagaa aaggtcgatc ccttgcccgg gcttgatttt acctcgatgg tgcttccatg
    46141 caactccacc agttgtttgg taacagcaag tccaaggccg gttcctccct gcaaacgggt
    46201 atcggaccca tccagttgct caaacgattt aaaaatggca tctattttat cctccgggat
    46261 accgatccca gtatcctcca cccggatttc aacctgctta tctttcactt cagcggtgac
    46321 ggtgatcttt ccctggtcgg tgaattttat ggcattgccg acaaaattga acaggatctg
    46381 ctgcaaccgg ttctcatctc ccttgacgag ggggatatct ctggctatgc tgttttcaat
    46441 acggaccggc ttcgtgccga tgagtacttc ggaagatttg atcaccaggt cggcgatgac
    46501 ataaagatcc accggctgaa tctgtaattt gagatcctgg ttccgggctt tggagaaatc
    46561 caggatgtca ttgaccagat tggacagacg tttgccgctg gaggagatca aacggaggtt
    46621 atcgaccgtt tgttcgctga gattgccgca ggccccttcg atcaatgaat ccgaaagccc
    46681 gatgatgccg tttaggggag tcctcagttc atgggaggtg ttggccagga actggtcttt
    46741 gagttcatcg attttctgaa gttgcagggc tttctgtttt tcgtacttga tttgtttttg
    46801 gtgttgctgc aagcggtaaa tcaagtagga gcccaacagg accagcagaa acaataacga
    46861 aaatccaatg atgttattct ttgcccgtct ttgagcaata acctgtaaat ccgatttgat
    46921 tttatcctgt tttagtttaa tcaaggtttt caacataccc agtttgtcgg tctcctgggt
    46981 ctcatcatga ctttcattca aaacattata agatttcaaa tactgatagg ctttatcgga
    47041 tatgttgaga aaatgatagg cttccgataa attttttaac gctgcccttt gaatttgcca
    47101 atcgcccttt tgacgggaaa cctctaaacc tttgtttagg tatccgaggg cggaccggta
    47161 ctttttgtcc aggctcaggg tacggccgat ttcaacattc gcattcgcct gacccaacac
    47221 atttggcacc atagcataca tcgtgtctgc ctggaataaa tattccaaag cctttgaata
    47281 attcccctgg tctgcaaagg aaatgcccag gttgatgtta aaatctccaa ttaaattcgg
    47341 aagtccgctt tccttcacca tggtaagtcc ttgattgagg agatctatgt ttcgctgttt
    47401 ttccccttgt atttgataga cctcagcaag attcatatat aagtatggga ggaggccatg
    47461 atggccgatc tgttcagcga tttccttact cttggaataa tattcaaacg cttgctggac
    47521 atccttttgc ttgacagcca taatacccag gacgtttaaa acgttggcaa tgcccaggga
    47581 atcatttgaa tcttcataat tttttaaagc cagaaaataa tattccagtg cctgatagtc
    47641 aaggtgccga tccctgtaaa actcaccaag attatattga gaaacagcca acctgcccgg
    47701 ggggctacct aacttctctt taatttccag gcttttttca tatgcctcga tcgctttggg
    47761 aaaatcaccc tggtccctgt aaagatttcc aagattgttg taggtgctgg acaatccatc
    47821 ttcatcattc aacagttgtt tgatggaaag gctttcttga aagttgaaca atgcctccct
    47881 gaattgcgcg gattcataaa atagtatccc caggtaatta tgtgcccagg cttgttcggg
    47941 taagtccgaa taattttgta cccctcttaa atatttctca caaaaaatga tggccgaatc
    48001 ccggttctca agggaccggt atgcccggga caaggatttc agggacttga ttctcttgcg
    48061 atctttttca atttcgggat gaacagatat caactgaaga ccatactccc ggactttgtc
    48121 atactcattt tttgcctctg aactatccat cccttcaata aggatggaaa ttttttcttc
    48181 gccccgggcc cgttccagcc ggctttcaaa atctccggcc tgggcggaaa tgaaaaacgg
    48241 caggagggtc caaaagaaca aaaacggaaa ggagcggaaa atataaaagg gtatacgttc
    48301 cataagcgga atttaattat gctatttacc caaacctcca attaccttat acctaagata
    48361 atctattatt ctctaaatgt aaatgacgca gaaaaagtaa cctggtcctt aaataataaa
    48421 acaggtgtca ttaccattca atacgaatga tcagtatcgg aaggaatgac gcctgcggag
    48481 taaaatatgt ttaacccaaa aatcataacc tccattttag gggtatccca caagcctttc
    48541 attccgtctc ctggtgatga tggcagggta gtccttaata tagtagaatc agggcgggat
    48601 aagcatgcgt gagtcaattt caattcaaaa gcagagcttg ggctgacacc ttgatcgttt
    48661 caattccttg ggttccaaat tatatatcag cgtatctcct gtttgagatt cgaattcctc
    48721 tacttaacat aatattaatt atgggcaatt attttaggga tatttttata cgggtatcag
    48781 ctaggaagct gatttacagc ataataccca taagatgaaa tccgttaatt ttgaatgatg
    48841 cgaaaaggac tcaaattctt gctatatgct ttgggcgtct tggtcatcct tttgttgtca
    48901 atgcccattt tattcaaagg gaaaatttcc aacctgatcc gcaatgaaat caatcaacga
    48961 attcatggag aactggcttt tagtaaagca ggcctttcgt tattccgttc ctttccggat
    49021 ttgtccgtat cggtttatga tgtggtggtt tccggcgccg atacctttgc acagacccgg
    49081 ttgtttgaag ccaggtctgt tcaggtcact ttagacttgt ttaaattgct caggcaagga
    49141 cttgacggcg tccaattgaa aaaaatccat ctccgggacc cggttttaag attggtcagc
    49201 acgccgtccg gaacctttaa ctataacgat ctttatcaaa cgaccgcaga ttcaggcgaa
    49261 gcaggatcca aactggaaat ccagctgagc tccataaaaa tcgaccaggg agccttccat
    49321 tattatgaca gcgccggcca ggtattcctt tcattcaatc acctggacca tcaaagttca
    49381 ggtcattatt caggcaccgg aggttcactg ctcagcctgg aacataaaaa tgtatttgat
    49441 tccttgacga tccggtacgg ggaaacggag tatctgagcg aactgaaagg tctatgggat
    49501 ggaaaactaa gcgccaacct ggattcgggc agctatgcct tgtcggaaga ccatctcagg
    49561 ctcaatgatt tgcagtttga tcttaatctt ttcctgcatt tgcaggagga caaaacgcgg
    49621 atggacatcc aggtaaaaac ccctggaaac gacctacgcc aattggtatc aataattcct
    49681 ggcgcttacc agaaaaactt tgacaaaatc agcacttccg gggaattcag cctggaaggt
    49741 aagctgaaag ggtattacag tccatccgga agcaggccgg aaatggatat aaaagccacc
    49801 ataaaccagg gtatggtaaa atacagccat ctcccctacc cgctcgaaaa catcaacctg
    49861 gacctccggg tcaagtctct cgataccttg ttcgaccact tattgattca attgcccaaa
    49921 ttcagcttca acttgcagaa caaccccctg gatggccacc ttacgatcga ccacaaatct
    49981 tccggcaacc aggtagaggg cttactcagg ggcaatatgc aattggccga tttccatcaa
    50041 gccattcccc tggattcgat aaaactggcc ggaaacctga acctggacat gcaatttaat
    50101 tttaatgatc aggcgcttca aaacaaagat tttgataaga taaagctggc gggtttcgcc
    50161 gaagccgaaa aactggacct caggtattat agttatcccc ggttaatgat aaacaggttg
    50221 gtcgccaccc tgcatcctaa aaatgtggat gtccggctta tggcggtgaa atttggacaa
    50281 tccgacctgg aaggaaatgc aacggtattc catcccctgg cctggttttc aaaacatcag
    50341 tctcttacag aaattagcat tcagtcaagt agcaatacat tgaatataag cgaattaaac
    50401 tattcttctt ccagcgttac ttgcgatact tgcatgtctt ctgaagctca caagattccc
    50461 cagctcccgg tccttaacct gaactccaag gcttccaggg tgatcttcag ggattatgac
    50521 ctcgaggaca tccatttcca gggaacgtat cgacgggata ccttatatat tcaatctctg
    50581 aatggtaata ttaaccagtc caggttaatg gtcaccggcc gcttggataa tcccaatgcc
    50641 tggtcatccg atcaggcgat tctttccggc tacctgggac taaaaacgga agtgttcctg
    50701 gtggatccct ggatggcggc gggcgatcca caggaagtta ctggagatac ggcgtattct
    50761 aaaaaattaa ttccacgtac agcgctttcc atttcacctg aaataaaact gctgaaatac
    50821 ggtaaatacc aggtccggga catccgcgga agactggatt tgatccagca gacgctggaa
    50881 gttcatgaag gttccggcaa gctttttaac gggacggtga atctcgacgg gactttcagc
    50941 gaaacgggcg ccctgcccat ttttaatttc aaattagaca tgagtcgttt aggtttcagc
    51001 gaaatgttca gggtctccca aaccttctcc aaacttgctc ccatagcgga attcctggaa
    51061 ggcgtttttt cagcgtcgat ggccctgagc ggccggttgg accaaaacca actgcccgta
    51121 tggaatgaat tgaatgccgc gggactggtc gaaacggcga ccgctgttct gtcgaaatac
    51181 aaaccattgg aagacctggc taaggccatt cagctccctt tgcttgacct acttaaatgg
    51241 gaaaaatcaa gaaactggtt cgaggtcatc aatgggatcc tgtatctcaa accctttgaa
    51301 atccgatatc agggcattcc catttccatt tccggttcgc acagcctcaa ccaggaaatc
    51361 aattataacc tgcttttcca gataccccgc aaactttttg acaaatacca ggtaggcctg
    51421 gtttccaatc aaaaactgga ttggttgatc ggggaagtta agaaaaaagg cctcgaccca
    51481 ggccggctgg atactctcaa catcaatctt ggtctgaccg gccctttgcg taacccttca
    51541 tccaaactgc agtggatggc ggatgcccag cacaccatga aagaagaagt gaccgcagcc
    51601 attgaggcga cggttcagga aaaagtcgat tcactggaaa accttgccag ggaaaaagcg
    51661 gaaaaggtga gggacagtat tgtggaaacc cttcgtaaag aaacggaaga acaaaaagcc
    51721 cggctggata gcctggcgcg gtctgctgtc gataccctca cgggaaaggt ggcagaaaaa
    51781 gccaaagagg cgctggattc caccctgaaa aaggaatcgg aaaagatcct ggattccctg
    51841 ctccggcaac agaccgattc cttgctcggt aaaaaagcga aagaagaaat tgacaagatc
    51901 aatgaaaaac tgaaaaattg ggacccattc aggaaaaaac caacgtcgga aaagaagaat
    51961 gaatagattg acctgggtgc ctttgatcct tgcagccttc ctggcaagca cggcatgtta
    52021 tccgccccgg aacgccatgc agaaaaccgc ccggaaatac cagagcggaa agctgacgga
    52081 agatacctct tacatatacc aattgccctt cagagcaggt accaaatcca gggtgatcca
    52141 gggatatttc agccggttta cccacaagca ccgggctgcc ctggacttca ggatgcgccc
    52201 gggaacagag atccgggcag cgcgttcggg catcgtgatc cgcaccaaag acgattccaa
    52261 ccagggggga tggaacaaaa agtacaggcc ggatgcaaat tttgttatga tcgaacacga
    52321 ggacagctcc agagcaagct atagacatct caaataccag ggcgtactgg tcaaagaagg
    52381 cgagaaggtc accaccggcc aggttctcgg atacagcggc aaaaccggat attccgcctt
    52441 accccatttg cattttatgg tgtcggtctt tcaggagggg cgctgggttc aaatccccac
    52501 ccgatttgaa tcaaatcccg aaaccggata cctcaggccc tggaggaaat acaaatccgt
    52561 aaatgtaccc cggtaccgat aagacggctt tcagcgattg tttttcaaat atccgaccag
    52621 atgtgcaagg tctacatccc ggagcattaa gttctgggga ggaggcatca ggctgcctcg
    52681 caagggataa cggccaacaa tggttttttg cgggatgtcg tgcaccttgc ccaggatatc
    52741 cttgagccgg acagggtccc cgtcactcat tacaaagccg gtgtattcca tcccgtctgt
    52801 tgtcttgatg gccgtggctt cataaccaaa aacaatgccc gccccgggat taatgatggc
    52861 atccagcagg gaggatacat ccatttttcc ggccacggca ctgaggtccg ggcccacact
    52921 gcctcctttt cctccgaatt gatgacaatg aatgcaggcc ctgttgaata cgagtcggcc
    52981 gctgtcttca ttcatttcca tccgtttgat cacatcaatg gaatactggt atttattttt
    53041 ccagaaaaga tctccggcca ttgcccggac gaccgggttg ggattgtaaa aaagggtatc
    53101 agaaatttgc ctggaaaggg cgggatccag tttgccgttg gagattaatt gcaggatcaa
    53161 aaggccaccc gagggatcca aggccagttc caaggctgct tctaagcggg cgcctccttt
    53221 ttctgtcatc aagcggttca gtccctcatt ccatatcctg gtttcggggt cgacgatcag
    53281 ggaatcgtag gcgccccagt caaccaggtc ctgccagtcg ttggtcctgc gaaacgcaag
    53341 ccaccagcgg gcttcggaga ccagggagga atcctccgac cggatcaatc cggccatgcc
    53401 ctttaccgcg cagggcgatg ggataaaggc aagggttccc aaaagtttaa tctttgtgga
    53461 atctttaccg ggtaaagaaa gccggtggac aatgtcttcg caggccccgg gagggcgtat
    53521 ggcatggagc acggcaacca ttgcaggctt ccagtttgtg gaatccagca ggggtttcag
    53581 tatttgggag tatacggagt cctcttttct tttgcaggca gcggcaatgg cagccagcat
    53641 ataagcatcg ggatcctggt attgaccgat caaatctttc aacagagacc gggcatccgc
    53701 gaatttcatt cccctgacgg caaaggcaat ttcgcggttg agtaagggat tttttttccg
    53761 gatcagctgc tgtgcatatt ccgggacatg actggaatcg gcctgcctca gcgccctgaa
    53821 agcaaccata tttttttcag gatcgcggtg atccagcaaa cggatggctt cagccttccc
    53881 tttttccccg agggtcgcaa gcaaccagat cgcccgcatg cggatgtacg gattggggtc
    53941 cttcaataag gtttgtaccc tgggtatcat ggacggtccc agggcccgta ttttttcaaa
    54001 agcctggtaa cgaacattga tggctgggtt ccgcaccgct tcaatcagcc cggcaggggt
    54061 atcaaaacgg agagcggggg attgaagaga tttgtttttt ggttggatcc tgtaaatcct
    54121 tccataccca ttcctttcac gcatctggtg gcccccgacg atgggatcat accaatcggc
    54181 aatatagatc gctccgtcgg tccctataca aaggtcgctg ggccggaacc acatggattt
    54241 ctccagggtg tcttcatgcc atttgtaatt gatgttgtcg gcctccacgg aaccgatcag
    54301 attgaagcgc tcaaaccggt accctgctcc ggaacgtttt ggtttgtatg cataaatgac
    54361 gttacgtccc gcttccgcac tcaacaagat tccccggtat ttatccccaa gagcatcgcc
    54421 ttcattgatg acgataccgg tcggggaccc cgcccccgta ttatctcctg caggcatcac
    54481 cc
//
LOCUS       DAOQXT010000021        53225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_670_length_53225_cov_6.681004, whole genome shotgun sequence.
ACCESSION   DAOQXT010000021 DAOQXT010000000
VERSION     DAOQXT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 53225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53225
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_670_length_53225_cov_6.681004"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PKM27_05410"
     CDS             complement(<1..538)
                     /locus_tag="PKM27_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR06735.1"
                     /translation="MDIRNIAIIAHVDHGKTTLVDRILQLSKVFRDNEETGELIMDSN
                     ELERERGITIFSKNASVVYKDTKINVIDTPGHSDFGGEVERVLKMADGVLLLVDAFEG
                     TMPQTRFVLHKALELNLNPIVVINKVDKPNCRPEEVHDQIFELFFNLGATEKQLDFPT
                     YYGSGKQGWFNDSLTPSQD"
     gene            708..1916
                     /locus_tag="PKM27_05415"
     CDS             708..1916
                     /locus_tag="PKM27_05415"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR06736.1"
                     /translation="MDSRFDKIFHFLARIGADVGDDEELKLQKSLLVLSTFPFMVAGA
                     GWGILYLYFGEIRAGLIPLSYSVISLFSVLYFGRNLRFEFFRFSQLLLILLLPFFLML
                     ALGGFVKGSAVILWGLISPLGALLFDTRERAPRWFMAYLLLVLVSGFLEPWLDVRNNL
                     NTWQINFFFTVNLGSVGALIFLMVSYFVREKNLFQQKSESLLLNILPKEIVTKLKQGH
                     QTIAEHYDSASILFADVVNFTPVSATLTPSELIELLNAVFQKFDELADKYGVEKIKTI
                     GDCYMAAAGVPRPRKDHAVILTAMAVEMRDYIRQHDFLGKKLQFRFGINSGPLIAGVI
                     GSKKFSYDLWGDSVNIASRMESHGTGGLIQITQATYELVKDHFTCTSLGTVQVKGKGE
                     MHVWEVVHRI"
     gene            2394..6740
                     /locus_tag="PKM27_05420"
     CDS             2394..6740
                     /locus_tag="PKM27_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNR06737.1"
                     /translation="MLNSIILFSIKNKIIIALLTLTLVIWGLWSASKLPIDAVPDITN
                     NQVQIITICPTLAGQEVEQFVTYPVEKSLANLPDVVELRSISRFGLSVVTVVFEDQVN
                     IWFARQLVGEKIREAEEQIPPGFGIPELAPVTTGLGEIYQYVIHPKEGSAGKYDAMDL
                     RTMQDWIVARQLYGTPGVAEVNSFGGLLKQYEVAVRPDRLRAMNTTMSEIFAALENNN
                     ENTGGAYIDKKPNAYFIRGIGLVTSLEDVKSIVVKNAPNGVPILIGDLAAVRFGSAIR
                     YGAISWNGEKEAVGGIVLMLKGANSAEVVSRVKDKMVKIQKSLPDDVVIEPFLDRTEL
                     VGRAISTVEKNLIEGALIVIFVLVLFLGNFRAGMIVASAIPLSMLFALGMMNVFGVSA
                     NLMSLGAIDFGLIVDGAVIIVEATMHHLGLRKTGAELTRAEMDAEVYKSASKIRNSAA
                     FGEIIILVVYIPILTLTGIEGKMFRPMAQTVGFAILGALILSLTYIPMMSAQFLSRKI
                     NHRKTIADRLMGWFNKIYSPVLQGAIRFKGLLVTATLVIFVFSIWLFSRLGGEFIPQL
                     EEGDFAFHCILPTGSSLSQSIETSMQAERILRQFPEVKMVVGKTGSAEVPTDPMPPEA
                     SDMMIILKPKEEWTTTRDSKVLASRMLEALEAIPGIFFEANQPIQMRFNELMTGIRQD
                     VAIKVFGENIDTLASLAPKVAELLTSVNGISSPQVERTIGLPQITIRHDRGRLANYGI
                     SIEEVNRTISAAFAGEKAGVVFENERRFDLVLRLDSAYRTSIEDVRELLVPTPSGSQI
                     PISQVADVSFRQGPAQISRDDGKRRIVIGFNVLGRDVESVVQEIQTLLEDRLGLPEGY
                     YYTFGGTFENLEKAKARLLLAVPAALALIFILLYFTFNSLKEAGLIFTAIPMSAIGGV
                     FALWMRGMPFSISAGVGFIALFGVAVLNGIVLIATFNQLEKEGVHDLIQRVREGTKIR
                     LRPVLMTALVASLGFLPMALSTGAGAEVQKPLATVVIGGLISATFLTLVVLPLLYMLF
                     IPGHAGRRMRIKPVTMSILIIMSLPPMTAFAQENRPVSLDEAVRQAKASNLILKAGTY
                     KITSLEALRPTAYDIPKTNLEMQLGQYNSVRFDQGFIFRQELPNPRNKQAGLSLVQAN
                     IRSAELAKAITENDLVYGVRSAWYEMVYLMEKQRVMESQDSILTQFVQAAELRLRTGE
                     TSVLERSTAISQSGEIRNQLAGVLADFEIARSRLQTLMVSQEWVAPPSGTPLEKRSFE
                     STDAVEGNPVLAWYQQQVKISEAKVTVEQAKRAPDFTLGYLNQSLRSLAGVEPGYSLT
                     DRFHVIQGGLAISLFGKSQKARIKAAEVDIEMAQANAEAQSWELKGALRQSLQLLERQ
                     RRSLDYFEQTALSSSGQILSAAQTSFRAGEIDYVEYVQALNRSYQVRSGYLDALNSYN
                     QAVIRCQYLINQ"
     gene            6765..8111
                     /locus_tag="PKM27_05425"
     CDS             6765..8111
                     /locus_tag="PKM27_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR06738.1"
                     /translation="MKHKISFMSFAVILALIIPACKAKIQDEHGHSHSDEAAAMGESG
                     EHHEEHTTSVSLTQKQSESIGLRLGGLEHKILNSGLKANGFLKVPNQNKATITSIYGG
                     IVKNILVMPGSRVSAGQTVAQILYPPLIPMQEEFLSLSPKIELASAELERQVELSNEQ
                     AGAFKNKQNAEANLKNLMIRRASLEQQINQMGIRADQLAIDHLVTILDIKSPISGWVG
                     KVFVNIGSSVDASTPIAEVMDNRSLHLDLFVYEKDLHEVRQDQLIDFVLTNQPGKSYT
                     AQVFSIGNTFENENKAIAVHAVVKGNKQGLIDGMNVTALISLKNATVPALPDEAFVNQ
                     DGVDFVFVQKVAGAEVHDEHHHEEGDIAHQNEHHEDGHQQDPGEDGHEHKAEEKTARN
                     GAEVLFEKIPIKKGVSDLGYTEVTLLKEVPLNARFVTKGAFFLLAKMSNAGEAHAH"
     gene            8203..10245
                     /locus_tag="PKM27_05430"
     CDS             8203..10245
                     /locus_tag="PKM27_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNR06739.1"
                     /translation="MDHQHVYDQDGRQLCCTLEEKVYSRAGANALIQGKSLAEKHEGV
                     RKVSDEKSHEHDTRAQGLTIFILPFISLILLLLGIGFDEGLIPNPGFFTGSTRLIWYA
                     LSYFPVGFPVLKEAFWSVLKGEVFTEFLLMSLATIGAFVIGEYPEGVAVMLFYSIGEI
                     LQTMAVRRAQGNIKALIDQRPDEVTVFEDNKLLNKKASDVAIGSIVLLKPGDQVALDG
                     VLWSESAGFNTAALTGESAPRNLQKGEPVLAGMINLNRVSQVKVNAKYQDSKLSKILD
                     LVQHATAQKAPTELFIRKFAKIYTPAVVLLACLIVLLPLFFTESYRFEEWMYRALVFL
                     VISCPCALVISIPLGYFGGIGAASRHGILFKGSNFLDIMASVQNIVMDKTGTMTKGVF
                     KVQSEQIMPGFDREQVMHAVNALEAQSTHPIASAIADHAGPVLPDQEVTKVLEIPGHG
                     LSGEVNGNPVLVGNFKLLKKYSVPYEMDPSLIDDTTIAIAMNEKFAGYLTIADEQKED
                     AQTAVDQLKALGVKLTMLSGDKTSVVSKVASRLGIQNAFGDLLPEDKVNKIKEIKARQ
                     ESVAFVGDGVNDAPVVALSDVGIAMGGLGSDATVETADVVIQDDRPSKIPAAIRIGKL
                     TKRIVWQNVSLAFSVKILVLGIGAGGLATMWEAVFADVGVALIAILNAVRIQRLQF"
     gene            complement(10308..11426)
                     /locus_tag="PKM27_05435"
     CDS             complement(10308..11426)
                     /locus_tag="PKM27_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR06740.1"
                     /translation="MNIKQGFQILVIGFLFFPLQAQQSGAGNGDAKIMDFEKYDPPST
                     LVVPEHKLTAAKFPFIDVHSHHWRNIGKDYFTKLSGEMDAMNMSVIVNLSGGTGENLK
                     KMVDSANIYMPGRFVVFANINIRSIDDSDWAENTVKALEMDVKNGARGLKIYKSQGMD
                     DKDSQGKRIRIDDPRIGPVWEKCGELGIPVLIHSGDPASFWKPADENNERWLELKTRS
                     GRTRENFEASWEQVMTEHMNIIRKHRKTTFISAHMGWYPNDLDKLAKLLDEMPNMNVE
                     IGAVIAELGRQPRHAKAFFTKYQDRILFGKDSYVVDEFPTYFRVLETGDEYFPYHKRY
                     HAFWKMYGMQLDDEVLRKVYYKNALRLIPGLKSDSFPK"
     gene            11509..12087
                     /locus_tag="PKM27_05440"
     CDS             11509..12087
                     /locus_tag="PKM27_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNR06741.1"
                     /translation="MQINDISRLIQSRRAYTPKFYSDLPIDPSWIEIMLENANWAPTH
                     KRTEPWRFRVFHSAESRQRLSDFIAEDYTRQTPADLYSEVKLRDMADKPLASGAVIAL
                     CMQRDPAQSLPEWEEIAAVACSVQNLWLSCSALGLGGYWASPGFITRLGPILELAEGE
                     KCLGLFYMGHLKPGTVLPATRKPVAEKIRWMK"
     gene            12165..14327
                     /locus_tag="PKM27_05445"
     CDS             12165..14327
                     /locus_tag="PKM27_05445"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNR06742.1"
                     /translation="MAQTLMLLIPVLGILGLLYTYLKSNWVANQDAGTEKMQAIAKAI
                     AEGAMAFLKAEYKVLVWFVLAVGALMAFGGSTTESSSILVGLSFIIGAGCSVLAGYIG
                     MRVATKSNVRTTAAARTSLTGALKVAFAGGSVMGMGVVGLGVLGLSGLFLLYSSMGWD
                     INKIITVITGFSFGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDHPLNPATI
                     ADNVGDNVGDVAGMGADLFESYVGSIIGTMVLGATFIGIAGYENTNSFNGLNAVLLPL
                     VLSAVGILTSILGTFFVNTKEGGNPQKALNTGVNLSSLIMLALTFVITRWMLPESWEF
                     NGITYTSLGVFWAVVLGLVSGVAIGYVTEYYTGTGTKPVKSIVKQSLTGSATNIIAGL
                     GVGMQSTAIPTLILAAAIIGSHYFAGLYGIAIAAVGMLSNTGIQLAVDAYGPVADNAG
                     GIAEMSELPKEVRKRTDTLDAVGNTTAAIGKGFAIASAALTALALFAAYMSSAKLNAI
                     DISHPMVMSGLLLGAMLPFLFSSLAMGAVGKAAMDMIQEVRRQFNEIPALKAALGIMR
                     KNEGKEMSEWSEDDKHTFEAAHGKAEYGKCVAISTQAAVKQMLLPGLLAVIAPIFIGF
                     GFGKEMLGGLLAGVTVTGVLMAIFQSNAGGAWDNAKKMFEGGVEIEGKTYYKGSDPHK
                     AAVVGDTVGDPFKDTSGPSLNILLKLISVIALVIAPYL"
     gene            complement(14687..14998)
                     /locus_tag="PKM27_05450"
     CDS             complement(14687..14998)
                     /locus_tag="PKM27_05450"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HNR06743.1"
                     /translation="MAISKKYLKSKNICKVTFKVEAEKVLKAKKVELLGSFNDWKSSK
                     EAVMKKLKDGSFTKTLDLSSPQEYQFKYVIDGKVWEVEPHADRLEYNGYGADQNSVIV
                     L"
     gene            15362..16183
                     /locus_tag="PKM27_05455"
     CDS             15362..16183
                     /locus_tag="PKM27_05455"
                     /inference="COORDINATES: protein motif:HMM:NF027202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BF3164 family lipoprotein"
                     /protein_id="HNR06744.1"
                     /translation="MMRPGIFIFIFLLAVSCQTGPNDQKSWHHTRTYPVSNINPVSIS
                     GDPSNLFVSSGARTEIHKFNYDGGLIEAIKNIRKPKYINILNPGELLVAESSAHVATR
                     VVNQNSIHTIPTNEYLDTPYGVSGAGNSLVITDYYKNKVYYNKGGQILSFGRPGIGEG
                     EFNGPTDIQLANNKIYVCDSRNNRVQVFDENGKYLLAVGEEDQIKTASGLFVTPDEII
                     VTDYTGNRVLIYDQKGKLKQSLTEPFDQPSDVFVRGREMFVVNYYANSIEVFTKY"
     gene            complement(16225..18357)
                     /locus_tag="PKM27_05460"
     CDS             complement(16225..18357)
                     /locus_tag="PKM27_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR06745.1"
                     /translation="MNFETKDLRNVVLLGHAHSGKTTMIECMLYEAHGITRRGNVEEG
                     NTVSDYNPLEQERKSSLFSSLEHTIWKGSKINIIDTPGSDDFIGEVISALKVADTAMM
                     VLNAAHGVEVGTELMWEYVEKFNTPSLFVINQLDHEKADFDNTLEQAVSRFGKKVVPI
                     QYPLNQGAGFHSIVDALRMVVYEFPPDGGKPIKKDIPESEKEKALELHNKLVEAAAEN
                     EEELMTRFFEQGTLSEEDLAYGLRMAIAHQSLFPVFCASARNNMGTGRIMGFVHDICP
                     SPADRPAAPLEGGGNLPCNSEDQTTIFIYKTITEPKVGNVSYFKVYAGKLKTGDELVN
                     ASNRNSERFGQLYIANGKNRESVDTLYAGDIGVVVKLKDSHTNNTLNVKGLDRKIEPI
                     HFPEPRIRVAVVPPSKNDVEKLAKALHIIQEEDPTVIVEQSAELKQMLLYGQGQLHLD
                     MVRQRVEKGQDIRMDFVKPRIPYRETITRVANGDYRHKKQSGGAGQFAEVHMRIEPYY
                     EGMPDPSDLTVRNREEDIMPWGGKLVFYWCIVGGSIDAKFSNAIKKGVLNKMSEGPLT
                     GSRCRDIRVCIYDGKMHPVDSNDMAFQIAGTMAFKNNFQNAGPQLLEPIYDLEILCSD
                     TSMGDIMGDLQTRRAIIMGMDRDGHYQKIKARVPLAEIDNYGSTLRSLTQGRAKFSLK
                     FAEYGPVPAELQRKLTEEYAREYEEEHA"
     gene            complement(18589..19698)
                     /locus_tag="PKM27_05465"
     CDS             complement(18589..19698)
                     /locus_tag="PKM27_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06746.1"
                     /translation="MNIHSRNLALDVFRGLTVCFMIIVNTPGTWEYTYAPLQHASWHG
                     FTPTDLVFPSFLFAVGSSLYFSRRSWEHAAPAAVYRRILKRTFIIFLLGFLMYWFPFV
                     RRAEGGLEFSPFSDTRILGVLQRIALCYGVAALMVFKLDWKKIVGISLGLLLAYWGLM
                     AWGGDYTMLGNLGQKIDLAILGEKHMYHGEGVAFEPEGILSTLPSLVNVLFGFLVTVY
                     LEEIGRKPSQLVRLFAAGVVLILVSIAWHQVFPINKKLWTSSFVLQTVGWDMILLSLL
                     VYAVDKIAWKGWTRFFLVPGRNPLAVYLFSELLAILLWFFQTGSGKSFYSSIYESVFQ
                     PLGNYSGSFLFAISFMLVCWLFGYYLDRKGIYLKA"
     gene            19810..20673
                     /locus_tag="PKM27_05470"
     CDS             19810..20673
                     /locus_tag="PKM27_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNR06747.1"
                     /translation="MKSFLFLALFFLFKPVFSKGQDFEIPEDTARLDIGKLARHILPD
                     STATTYARVSAVVSWTNTRFAWTYTDYKRRTVKEIICRRGGNCNEQARVVSALLTEAG
                     IQNRRVSEINIQPESTQRQESAEKKIKENGFRMSVFGLRHNDHVWIEYFDENLKAWEP
                     ADPTLGLVGLEAWIKARAGFGQRINHDILPSRDMLVPIAVFALGPDGTILENRSRHYL
                     VNAFNQVYEKKLSAGPAWSGWTEEVLNLEQVCRQAFEGRVNLHEHTDQIKKIREEYQS
                     LRTWYIDRHEP"
     gene            20727..20939
                     /locus_tag="PKM27_05475"
     CDS             20727..20939
                     /locus_tag="PKM27_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06748.1"
                     /translation="MPGCGEGGYKPRFAFEVLSAGRQKEVVDLVNALALYKPTKIAVE
                     WIKEEDQAFTDSLYQEYLEGRRDGVF"
     gene            complement(20992..21357)
                     /locus_tag="PKM27_05480"
     CDS             complement(20992..21357)
                     /locus_tag="PKM27_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR06749.1"
                     /translation="MKRIIRASGHKEIFTEEKCFIDEIFNQKEYGLFSLARARVEPGV
                     TTALHSVQDTDEVYYILSGTGEVEIDGRIIGEVGAGDLVFIPRNSSQRIKNIAGEDLI
                     FLCVYAPRFDSGNYRKIGP"
     gene            21465..22604
                     /locus_tag="PKM27_05485"
     CDS             21465..22604
                     /locus_tag="PKM27_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNR06750.1"
                     /translation="MKEIKQIIKQYESAREKGIRTALATVVHLEGSSYRRPGARMLIE
                     ENGQLTGAISGGCLEGDALRKALHVIAERRAMLVTYDTMDEDDAKFGVGLGCNGIIQV
                     LIEPILDQDPLNPVQLLKILAGSREPAVLLTLFDLSDRKAVQHGTCLIYHTPHKVMGT
                     VPVFSDRIREDIDSALLQKSSLFRQYGDDNVQVTAFIEYVDPPVALIVVGAGNDVMPL
                     VDMANILGWEPHVVDGRANYATTDRFGSACSIIVAKPEKVLEQIRIDHRTVFVLMTHN
                     YNYDLAMLRALLDVETPYIGSLGPRKKLDRMLDELREEGIQITDQQRQKIFGPVGLDL
                     GAETSEEIALSILAEIEAVLHGTTGGQLRDAGEYIHSRESLKIIQ"
     gene            22753..23361
                     /locus_tag="PKM27_05490"
     CDS             22753..23361
                     /locus_tag="PKM27_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNR06751.1"
                     /translation="MTKAKKIGIVILAAGASRRLGSAKQLLKANDTQTLLNHAATIAL
                     STLFRPVVVVLGSNHEALANELSGLNLFLAINAQWQSGLASSVKCGLREVLNLQGDTD
                     ALIFMVCDQPYVTGPLLEALVTTYQASGKNIVASRYKEANGSPALFDRRFFPELEKLE
                     GDKGALRLIHQYPDQAGFVPFPKGQIDIDTVEDYLRYKNQEK"
     gene            23608..24021
                     /locus_tag="PKM27_05495"
     CDS             23608..24021
                     /locus_tag="PKM27_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06752.1"
                     /translation="MSLWSGLFLILLPKCPFCVIAYSGTIALCGNGPSAAPNAHENQL
                     ALALSVMLSLIIAAAIALNYRGWRTWMALGLAFTGMLVLNGSILKPAGYFWYYTGVVI
                     LFSGVWLNGSLLYLINRRRPLARAGRTLDYLQNIK"
     gene            24032..24895
                     /locus_tag="PKM27_05500"
     CDS             24032..24895
                     /locus_tag="PKM27_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNR06753.1"
                     /translation="MIAQPFQYEAPATLQDAIGLLQKYGDEAKILAGGHSLIPMMKLR
                     FATPGYLIDINNIPGLSSIQEENGFIRIGALVREAEVEHSALLTGHYPIFADSTRLIA
                     DPQVRNMGTIGGNLAHGDAANDHPAVMLALRARVVATGPKGSRTIAIDDFFQGFYTTA
                     LEPEEILTEIQIPIPPGHTGSAYYKLERKVGDYAIAGVAVVLTLDGNLVKSIGIGLTN
                     VNPVPMRASRSEEFLLGKPLTDENLAQAGKLAAEDCSPSTDLRGSEEYKRDMVAVVLR
                     RMVKLAAERAK"
     gene            24923..25420
                     /locus_tag="PKM27_05505"
     CDS             24923..25420
                     /locus_tag="PKM27_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR06754.1"
                     /translation="MKINVTVNGTAHEVEVEARTLLVHMIREILNLTGTHIGCDTSSC
                     GACTILLNGKSVKSCTVLAVQANGKSITTIEGLATAEGLHPLQEGFKENHGLHCGFCT
                     PGMILRASELLANNPNPTEEQIRWGISGNLCRCTGYNNIVKSIQYAAAKMRGDALPST
                     EPVFV"
     gene            25455..27821
                     /locus_tag="PKM27_05510"
     CDS             25455..27821
                     /locus_tag="PKM27_05510"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HNR06755.1"
                     /translation="MIQCKTSKAIGGMGHSLKRKEDVRFIQGAGHYVDDIKLDGMLYM
                     DIVRSPYAYAKIKNIDISEAMKVPGVAAVVTGKDLEKYNLHWMPTLMSDTQMVLPTDT
                     VMYQAQEVAAVIATSRYAARDGREAVKVEYEPMKPIMDPYKSMDPSSPVLRTDKPGKS
                     DNHIWHWEVGDKAKTDEVFKKAEVVVEQHMHIPRIHVASIETCGCVADYDKINNHLTM
                     YMTTQAPHAIRTVIALVAGHVGLTEEKIRVVSPDIGGGFGGKVPVYPGYVIAIAVSFL
                     TGKPVKWIEDRSENLQADSFARDYHITVEMAGTKAGKIQATRFKVLADHGYTDASANP
                     SKFHTGLFSIGTGSYDYDTAFMEADAVYTNKPPGGVAYRCSFRVTEAVQAIERITDLF
                     ALKVGKDPAQLRMENFIKKEDFPWKSPTGWTYDSGDYHAGLKKAMEAIGYDSLRKEQA
                     EKRAKGELMGIGISTFTEIVGAGPSKDFDILGIKMFDSAEIRVHPTGKAIARFGTKSQ
                     GQGHETTYAQIIAEELGIPAENIHVEEGDTDTAPYGLGTYASRSTPTAGAAAAVAARK
                     LRDKARKIAAYLLEVSEEDLEWEPGKFSVKGAPEKAKTIQEIVFAAYTNHPQGMEAGF
                     EATHYYDPPNLTFPSGAYICVVDIDKETGQIKVRRFVAIDDCGHIINPMIVQGQVHGG
                     LTQGIAPAMYEELIYDEDGNILNGTFMDYLVPTAVESPHWETGHVVTPSPHHPIGAKG
                     VGESPTVGAPPAIANAIMDALSPYGIHHIDIPITPFKVWKALKEKGVI"
     gene            27905..28432
                     /locus_tag="PKM27_05515"
     CDS             27905..28432
                     /locus_tag="PKM27_05515"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNR06756.1"
                     /translation="MNAKINKTIKLDHPVDAVWELISDPYKVVSCVPGASLTSQESEN
                     DYKGQVEFKLGPVKSKFDGKVTFLSKDPNLKKMALKGTGLDSKGKGSAEMSMDAEVKA
                     GTTGTEVSLNMDVSITGMLAQFGSRLINDVSSQLLDQFADNLKAKLSGGEVDTSIHTG
                     SIVGSVVKGLFGGKS"
     gene            28442..29320
                     /locus_tag="PKM27_05520"
     CDS             28442..29320
                     /locus_tag="PKM27_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNR06757.1"
                     /translation="MERFHPASPESIIKALSDLGYVAEEDLAIVLFLVLTLRKPLLLE
                     GPPGVGKTEVGLALSGLLKAPLIRLQCYEGLDLNQAVYEWNYQKQLLSIKLLESSGSS
                     LRDKEDLIFSEEYILKRPLLQAITQADQSPVLLIDEIDRADEEFEAFLLELLSAFQLS
                     IPEIGTVKATHKPYVILTSNRTRELSDALKRRCLYHWIDFPDFDKEMRIVEKKLPGIE
                     EKLLKQVVGFVQKLRSRELAKQPGISETLDWAESLMTLGYRALDQKTLDHTLGIILKS
                     NEDIKMIREEGIESLV"
     gene            29324..30466
                     /locus_tag="PKM27_05525"
     CDS             29324..30466
                     /locus_tag="PKM27_05525"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNR06758.1"
                     /translation="MYTQAYSARSISSGMVGFFRYLRSHGFLIGLRETTEALEVAHTS
                     LVRDKHRLKTALAALCCTSREELALFDRLFAAYWDTSPMDLAERKNQVTVRGQVRSKI
                     NTSLVLLGRGKGTGKEEEEATSVSGANAEERLRETDFSKIREIDEKPLEQLAERLFRE
                     FAVKMRRKLKATRKPGKVHLARTVRKSIESGGDPLKLFYTTRKLKKRRLVILLDVSGS
                     MDKYSFYLLRFIYALKQEFSNLEAFVFSTRLISITRALRNMKLDSVLREVSHFASHWS
                     GGTRIGECLRDFNRQYSKYVLHGSPAVIILSDGLETGNPVLLGSEMIKIRRRSKVLIW
                     LNPLKGMKDYQPLAGGMKAALPSVDEFRAAHNLKSLLELEDLLTHV"
     gene            30459..31433
                     /locus_tag="PKM27_05530"
     CDS             30459..31433
                     /locus_tag="PKM27_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNR06759.1"
                     /translation="MSDDYLNKLIELSKRNEAFASVTVVRREEPSSGKAGDKAIVNQQ
                     GELFGWIGGGCVKSIVCSEAMDAINTGKPRLVKIGQEGTLIQQDDFKSYKMTCHSEGS
                     VEVFIEPWLQSPHLIVIGKTAIAKALVRLAKAAGYRVTAVAPEAGPQTFEKVDELITQ
                     LNLSGVQSSPRSSILIATQGESDETALEMALQKEAGYIGFVASRKKKEAVWKFLKDQF
                     IPDAQLERVSCPAGLDIRAKSPEEVAISILAQIIQVQANAPVSPLKPEQKKEEKQYYI
                     NPVCGIPVDIHHPKHILEYQGEKVYFCCDGCKVQFEKEPEKYLPKSIA"
     gene            31430..32410
                     /gene="moaA"
                     /locus_tag="PKM27_05535"
     CDS             31430..32410
                     /gene="moaA"
                     /locus_tag="PKM27_05535"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNR06760.1"
                     /translation="MIQDKHGRVHTYLRISLTDNCNLRCFYCMPEEDYEFTPASRLMQ
                     AAEIETLARTFVDLGVNKIRLTGGEPLVRKDAADIILRLSRLPVKLTLTTNATRVHEF
                     IDVFKEAGIRSLNVSLDTLDRDRFLFMTRRDQFQRTLDNIRLLVDQGFHVKINMVVMK
                     GLNESEIPDFVAWTKKEPIHVRFIEFMPFEGNRWTSNKVFTLQEMLELIGSRFEIEAL
                     LNGIHDTDKKFRALGHAGTFAVISTMSSPFCAGCNRIRLTADGKIKNCLFSKTETDLL
                     TALRKGEDVVRLIHQNIGQKAKELGGQFDADIEHIHPEEIHNRSMITIGG"
     gene            32454..33626
                     /locus_tag="PKM27_05540"
     CDS             32454..33626
                     /locus_tag="PKM27_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06761.1"
                     /translation="MTTEEDQKNNEKIKQEESSHNFAKPADTSNPTDPFSLPIEYLPK
                     TPLWPGNNLDSHKKIINFSDSLKHSLKSLSEVLPKITPNITSSLIPKITPSIMPTLTP
                     TLNLLFKHQSEISDLESEIKNILIKNEQLQGEASLKDEKINELNKKVLELSHKQKLGY
                     LLSQVNDFGQKILLESEEFRNKFYNKENQKAFVVSIDLRRSTELMLKAKSPEHFEKFI
                     SELCESLSEIIKENFGIFDKFTGDGILAYFPLFYSGEDAGLFALKASIDSHKIFSNHY
                     EKHRSSFQAVLKNAGLGIGIDFGVTKLVNLINSHTVIGVPVVYACRMSSHYAGSTVLN
                     QSAYDELKKQNSIGLKFTEITLPVKNEDDFIGYLVESTGDDIKPKQPLWLKEIKED"
     gene            33745..34935
                     /locus_tag="PKM27_05545"
     CDS             33745..34935
                     /locus_tag="PKM27_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNR06762.1"
                     /translation="MNFISVQEAKFLILNQVDHPKPVAVHLDQATGKILAEDILSPLD
                     LPPFPQSSMDGYAFRADSYDPGKGLLITEVIPAGQGREILIGKGEAVRIFTGAAVPQG
                     ADTVIMQEKTEVRDGRLYLKEEVPPPGTHVRPAGSEIRKGEQALPTGHLLNPASIGLL
                     ASMGLTSVAVHTPPSVHILVTGDELTSPGEPLPYGRVFDSNSFTLRAAFQLAGIEDIR
                     ISQVPDREDLLSEAISSALEKQDLVVLTGGVSVGDFDFVAATAMRCGVEKVFHKIKQK
                     PGKPIFFGIKGNKLVFGLPGNPASALTCFYEYLEPAVQKLLGKSSAVRKVNAPIQSSY
                     KKAPGLTHFLKAWYDGSRVFPLDAQESFRLRSFARANALIVIDEKDDGVQAGEQVEVH
                     ILPV"
     gene            34947..35684
                     /locus_tag="PKM27_05550"
     CDS             34947..35684
                     /locus_tag="PKM27_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNR06763.1"
                     /translation="MDYAWWFYLILYVVAFLYASVGHGGASGYLALMALANAAPAEMR
                     PTALILNLFVSLTAFIQFFRGGHFKSGIFWPFILASVPMAFWGGRIHVDDEIYKQILG
                     VALLFPVARFLFFQNTPQENLKKSNTPLSLGIGGIIGFFSGLIGIGGGILLSPILLLL
                     KWTDQKQTAAISALFIFINSASGLAGQLTKGISISSQMYILVIVAFTGGLTGAYFGAS
                     RFDQKVLKTILAVVLLLAAVKLLFKVG"
     gene            36048..38777
                     /locus_tag="PKM27_05555"
     CDS             36048..38777
                     /locus_tag="PKM27_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNR06764.1"
                     /translation="MFNPISSLSVLVLLPFYLLSASPIRVDRMTCEYLENPLGIEILK
                     PRFAWTLTSPVKNQYQSAYEILVSDNAGSLDLGRGNQWSSGKILDSNTIQIEYGGIPL
                     KSFTRYYWKVRVWDQSGEASGWSKVAWFETAMLSPADWKASWIHDGRTPPDKDEEYYE
                     DDPMPLFRKKFNTVGAIASARLYLSGLGYSEAELNGKKIGDQVLDPGFTTYRKQAYYV
                     VHDITRLIKEGNNTLGVRLGNGWWNPLPLRMFGRWDLRAFQQSGRPCLRAEIHLTYQN
                     GRKEIIPSDPSWETAPGPVLFNNVYLGEKYDARLEKSFDDQAAWKKAVPAPGPDGTLT
                     AQLLPPIRITKEIRPVQIRPAGKDTFIVDMGQNFAGVARIRVQGPAGTKISMRFGEDT
                     MKDGRLNWWTTVAGQIKEVWGISGGPGAPKTAWQKDEYILKGEGVETWTPQFTFHGFR
                     YVEITGWPGQPGLDDILGLRMNTDLAAHGRFECSNLMFNRLHEVIQWTFLSNVFSVQS
                     DCPAREKMGYGADMVVSAEAFLYNYDMAHFYSKTVRDFANEQRPEGGITEIAPFTGIA
                     DRGYGDGSGPLGWQLAFPFLQKKLYEFYGDKRILEENYAVFERQMNFLKTKAVSNLFH
                     WDISDHEALDPKPEAFSAAAFYYHHAILMAEFSGILGKKENEAKYLKLASTIKNLIVS
                     KYHVPGTGRFDNATQSAQLFALWYRFAPDRAAAFQTLVQEFKRHNMHLSTGIFSTKFL
                     FDVLREEGQAELVYLLANQRDYPGWGYMLDQGATTLWETWAYPDRAPSQNHPMFGSID
                     EWFYRSILGINPSAPGFAKVILKPQPAGDLSWARGEYQSIRGVIKSEWRTDGNKYFLK
                     AFIPANVRAEIWIRCKPNGRIMENGKEVPGVKFTQGYAVVETGSGEYLFETEL"
     gene            39181..39423
                     /locus_tag="PKM27_05560"
     CDS             39181..39423
                     /locus_tag="PKM27_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNR06765.1"
                     /translation="MHPGKIKVLFFGQLAEMTGKSGMDLQDVTDTQAAIETLTRLYPR
                     LSQTPFLLALDKEIVEGKIPFTGDHTLALLPPYAGG"
     gene            39434..39877
                     /locus_tag="PKM27_05565"
     CDS             39434..39877
                     /locus_tag="PKM27_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNR06766.1"
                     /translation="MSSHRIRNIFVQGPISPAFIAEKIAAHSDKTNIGGHSIFLGQVR
                     ADEKEGKTVVSIDYTAYEEMALEKMHAIRQETFNKYDLTCMHVYHSLGQVKAGEISLF
                     VFTSSVHRKPAIDACAEIVERIKAELPVWGREILSDESHVWKENR"
     gene            40063..40971
                     /gene="moaCB"
                     /locus_tag="PKM27_05570"
     CDS             40063..40971
                     /gene="moaCB"
                     /locus_tag="PKM27_05570"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HNR06767.1"
                     /translation="MVDITHKSTSLRKAIAGAIVSVSKPETIEAIRNKTVPKGDIFEF
                     SRAAGLLAIKKTSDLIPDCHPLPIEFASISYDLKDLEVHITVEVHTLYKTGVEVEAMH
                     GASLTALTMYDMLKPIDKGVEIKNIRLISKKGGKSDFRKESYDHLQAAIIVCSDSVSK
                     GKAEDTSGKLAAEKLKTFGLQIKTQTVIPDDFDTIQDRARMYSRDHDLVLFIGGTGLS
                     PYDLTPDALKPLLDKEIPGIAETARQYGQQRMPYAMLSRGIAGFMDKALVITTPGSPS
                     GTRETIDALFPAVLHVFRIREGKRHD"
     gene            41040..41495
                     /locus_tag="PKM27_05575"
     CDS             41040..41495
                     /locus_tag="PKM27_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR06768.1"
                     /translation="MTYPNTISSTIPEFIEASVQSLRQIHADQWPTRSGPERWSKKEI
                     LGHLIDSAMTNIRRLVVTQYEANDRITYRQNEWVAYSAYQDAPVEDLITLWRLINLHY
                     HRVASNVPKSSLSLTCNTSNGEPLLRTLDFLITDYWGHQQHHLRQIFEG"
     gene            complement(41586..42629)
                     /locus_tag="PKM27_05580"
     CDS             complement(41586..42629)
                     /locus_tag="PKM27_05580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNR06769.1"
                     /translation="MELGISSFGDLSPVEQGWSQNGHRRMQELMEEIELADQLGLEVY
                     AVGEHHRPDYLVSAPAVVLAAAAVKTKGIRLSSAVSILSSDDPVRVFQQFATVDLLSS
                     GRAELMVGRGSFIESFPLFGYHLEDYDELFEEKLQLLLEINKQVKVSWKGKHRPDIPN
                     LGIYPRPFVEKLPVWIAAGGTPQSAIRAGTLGLPLTLAIIGGQPVQFVPFIDLYRKAW
                     DQHGHPSQKFQLGINSHTYVAETSQQAADEFFPSYAEVMSRIGRERGWGPTTRGQYEA
                     MRSPRGALLVGSPQQVIDKILYEHELFGNTRFMAQMSIGTIEHKKVMRSIELFGTVVA
                     PEVRRQAVSQLVS"
     gene            42750..45155
                     /locus_tag="PKM27_05585"
     CDS             42750..45155
                     /locus_tag="PKM27_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNR06770.1"
                     /translation="MSIKTGLSFLFLIIYNSLSGQSVCYFNAKVVDDQTGDPLAGVVM
                     RMDGLDTYRTSDSLGSLRMGVKCGEHTVNLQAYGYKPFSYRYNVQGDKPVEIRMESLL
                     TQLEEIVISSQSESRNIESPALGVSMMNLKAVQKLPPAAGELDVLRSLQSLPGVSAVG
                     EGANGINIRGGSVDQNLIFLEHMPVFNPTHLLGLFSLFPTDGIRELQLYKGSIPARYG
                     GRTAGVLDVRLAEPDMEKFNMKGGIGMISNRLHAEVPLIKEKMGWMSSNRFSFNDYLI
                     KLYNAALTPFGNKRIPNNKPQFMDLANKISWRPTGRDNISLVSYISYDAYLIDSLYSI
                     AGIVPKQANMKYGHNNFSARWNHYFSPKFNFNLLAVQSRYNTSTSSSEIGTGFDFKTG
                     LVYQNIKAELTYAPTALQRINAGFSVTRLRLKPGSLKPVEGSTLLPAELNRELGMEAA
                     FFISDEYEFNKRLLVEFGLRAVRFWNLGPVDLPLFEEGSPKSPSTITGRLNLNGAESA
                     YNRLEPRLAMRYKLNDLTSVKIGYNRMNQFLHMVSNNSTPLPNVRWKSTNRYLPPEQS
                     DLVSIGLFKDTRLRNWEWSLEGYYRWQGFIFDYVNGAELSINPDVETQILPGKGKAYG
                     AELLINKKKGIMTGWMSYTYARSFQKITGEYPEIQRLNEGRWFPSIIDKPHTLNILTN
                     FQTEKHNALSLTFTYSTGRPYTAPVSFYRNGFKYVPVFTDRHNGRISDYHRLDFSWII
                     TNPSMKQKRWEGSWIVTVYNLYGRKNAFSYFFNPDLALFKPFKVSVFPSPIFSVTYNF
                     KFE"
     gene            45157..46209
                     /locus_tag="PKM27_05590"
     CDS             45157..46209
                     /locus_tag="PKM27_05590"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNR06771.1"
                     /translation="MRLTLILGITLFLTCQYPVDFSDLPETQKFLVIEADVTEDHVRV
                     DVRYSLERVTSAGAYTLPPPPQATAFVHTGNGLIYQVSNTRGVLDTFFKGRVGQSYKL
                     VVHTEGKTYESTEETMRPCPELDTLVVQYSRESFRDKADLLYDGYDVYARASDIAGVE
                     NFYQWHWIHYSKAFQCNKIFDRAQNTEAYVPCFPHNCWNIVQNQRVIVQKDNLRDGQA
                     LTQSVVRVPYATPPNRYYLRVEQRSITPTVFDHLKSLETQTETVGTLFDVPAQTRFSP
                     NIVNTADRSEKILGVFNVYSYRYKIVVIDMKKEIAGAVVKTVIDRTPFHSNALLSAAC
                     TEETYRTQKRPEGWVD"
     gene            complement(46265..47584)
                     /locus_tag="PKM27_05595"
     CDS             complement(46265..47584)
                     /locus_tag="PKM27_05595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNR06772.1"
                     /translation="MDRTALWTNEYLDKMRFHGDPVADAAAEALFQDENQPNIFRFLK
                     DLARNDSPISPDLDPRLREYFEKTGTLLCTPEEELLLDESVAFFENHGFRILGMLFFK
                     SLPTGYMCEQPSKVLHETKLLVEQATRRVLETAQFIFDVSNPGWYKPEGSGIRAIQKV
                     RLMHAGMRHMILKDLEQPWDKNSVKEGVPNGVPINQVDKVLTCQLFSLAVLEGLKKMG
                     VKLSPREEEVHFFHWRVVGLMLGIEEELMPNTLQEGWYLQNQIYNRQINTVNQYGKTL
                     NDALMDVIAQHTGKFIDRERLQDVEVFFIENQKIFTSLGIPEPTFWDKFKSDIILHLI
                     SAKDNHHDESGGKLPMRKHGLLDRVFHFISKLFGYETEKSNAFKMDAFEFLCKTILAS
                     MTKSQEAAAAGGSNAPGAPAAPFVKTKPAVEGEVTLIRKWELEGWNI"
     gene            complement(47586..52727)
                     /locus_tag="PKM27_05600"
     CDS             complement(47586..52727)
                     /locus_tag="PKM27_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR06773.1"
                     /translation="MYEYSIGPLKIKTTRCNYFEATGRDGQPYLLELPASRLYDFESL
                     VGLKNDYEISSILESPQILKAHEIIRQEAGIGVIKEMFQGNTLESEIDKLHLSIPDFL
                     DLAYLLADCVGKVHARGVVHRDISPSSFLVSPDLKNIKLSYFGHATTLSTTAQRIDHS
                     VQDSISPFYMSPEQTGRMNRSIDYRTDIYSLGVLFYKLVTGRMPFDGNLSEIIHAHIA
                     KQAVEPHLINRAVPPIVSEFILRMMSKNAENRYQSISGLKTDIKEIKERVSKGHASGM
                     VLGEHDHYSKLRISEKLYGRNRELDVLMQSFNRAREGHTMGLMVSGYSGIGKTRLINE
                     VQKPIIHQNGFFVFGKFDQYQKDLPFNAFGQAFGSLVRYVLAQGQEAAQKIAGELQPI
                     LEDNGSLLTDIIPELNLLIGQQEAALKLGFTESRARFLKLFERFISVLATAEHPLVLF
                     IDDLQWADSGSLDLIEYLLLSPAIKYFMIIGAYRDNEVNLSHPLMMMLGRIREQRPDR
                     TNEIQLKELDGLSVNELLADTLHTSMEATARLSALLIKKSQGNPFFIRQFLEKLEEEK
                     LLYYSDADLEWKWNEAEIDKLAVTANVIDLVELKIKKLSKGGQFILNIAAFLGNRFDL
                     HNLSIASEYHVNQVSAALWESVEAGFVNPLGNWNKHYKDDLWEEIGVTGLQDNRQVFF
                     QFNHDRIQQAAYSLISPEQRAMTHLCVARLLNESYKNNPDRDMLFDVVNHFNNGSHLI
                     QNPEEINYVATLNYEAGERAKNSNAYIPALNYIRAAINLVQDEKSALYQDLLFSQAEA
                     EYLCGNFDQSEQVFDKAINRAGSALEKAELYAQKMRLYENTQRHAHAIDMAKKGLAQL
                     GIPLPVNPSQGQVMLELVRVKFKLRGKTPESLFHRPAMSDPALKLAMRILMNIWGPAY
                     LLQKQNLLVYFILKMVNYSITHGNAVESALAYAFYGFVVTAQLKDAENGFEFSRLGIR
                     LNEKFQDNSLRSKVYVIAEGCVSHWKQPYEAAMPGLQIAYQSGVATNDLIYAGYAVSF
                     MWRIPYYNGEHLESVYEKLLTYAHFSRKTHNTLVLHFFLSYARIVTQFLDREPSAEIF
                     HEYTGEKEHFAFIEDWAKTHKIPMPLAIYYFQKGYFHYVQGDFSRALDYFNLSEPMYP
                     AFLGLAEHPDLFIFQALTIAALLKQKPDARLAKKLKKSVKMIRKWALSSPQNFGAKYD
                     LILAEQAAAEADYRKAVSYYSSAERKARSRGMHQIAGMVHERWSELEYQLGINQHAEF
                     LIREAWIDYTNWGAYSKLKALEKKHPFLLEKNTGGRETVERAGTSSIDLNSVVRASNS
                     LSQEVVYEKLLISLLDIAVQNAGAQKGFFITPGREGLSVEAFISKEGTGQQVVVSSKL
                     SESPGLSRQVVNYVFNSRESLILNNASFDLRFARDSYIEKEKPLSVLCMPVMRNNEIV
                     AILYLENNLATDVFTEERLEILQLLSGQMAISLQNAELYETLEARVNERTATIENQKK
                     LLEEEKGRSDRLLLNMMPEEIAHELKTSGKSAPRQYSEVTILFTDFEGFSKMTKAMSA
                     TELVSFLDDCFRAFDQIMKDHGLEKIKTIGDAYMSVSGLPYPDPQHAHHAVRAALDIL
                     RFIERYNRDRLEKGLPYCQVRVGIHTGPVVAGIVGSDKFAYDLWGDTVNIAARLESYG
                     EPGRINISDITYHKVKDQFECQPRGKITVKHNEEIQMYFVNPNAE"
     gene            complement(52750..>53225)
                     /locus_tag="PKM27_05605"
     CDS             complement(52750..>53225)
                     /locus_tag="PKM27_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR06774.1"
                     /translation="EPASFDTVWAMESVCHANDKKAFLKEAFRLLKPGGRLAVADFFS
                     LPAGHEPQGRPLMQRWADAWAVPFFELSGRFSAMMEECGFKQINCGNITDKIKPSAQR
                     LYYYFFPGWVIGNLMRLMNQRNQTLQKNLWSTFYQYKALKKDLWNYLVFSAVKPH"
BASE COUNT        13258 a        13768 c        13374 g        12825 t
ORIGIN      
        1 tgtcctggct cggggtcagg gaatcgttga accagccttg tttgccggag ccgtagtaag
       61 tgggaaaatc aagttgtttt tcggtggcgc ccaggttaaa aaacaattca aaaatctggt
      121 catgcacttc ctccggccgg caattgggtt tatcgacttt attgatgacc acgatgggat
      181 tcagattaag ttccagggct ttgtgcagca caaaacgggt ctgcggcatg gtcccttcaa
      241 aagcgtcgac cagcaacaag accccatccg ccattttcaa cacccgctcc acttcgccgc
      301 caaaatcgga gtggccgggg gtatcgatca catttatttt ggtgtctttg tacaccacgg
      361 aggcattttt gctgaaaatg gtaatgcccc tttccctttc cagttcgttg gagtccatga
      421 tcagttcccc ggtttcctcg ttgtcccgga agaccttgct caattgcagg atccggtcca
      481 ccagggtggt tttaccatga tctacgtggg ctatgatggc gatgttgcgg atatccataa
      541 tcgattgatt ttgatctgaa aaggcgcgca aaggtagtca tttatccggt gtcttaccga
      601 aactgaatta aaataagttg ttaattggcc ctggcgaatg cctccgcggt cggggttcac
      661 tccgcccggc agaaccatgc tttcccttaa cttcgtgtga tttttccatg gattcccgtt
      721 ttgacaagat attccatttc cttgcccgga ttggggccga cgtgggggat gatgaggagt
      781 tgaagctgca aaaatcgttg ctcgtacttt ctaccttccc gtttatggtt gccggggccg
      841 gttggggcat cctttacctc tactttggag aaatccgggc gggcctcatt cccctgtcct
      901 attccgtcat ttcgttgttc agcgtattgt atttcgggcg aaacctgagg tttgaatttt
      961 tccggttcag tcagctgttg ctcatcctgt tgctgccttt tttccttatg ctggccctgg
     1021 ggggttttgt gaagggcagc gccgtgatct tatggggctt gatttctccc ctgggcgcct
     1081 tgttgtttga cacccgggaa cgggcgccgc ggtggttcat ggcttacctc ttgctcgttc
     1141 tggtctccgg attcctcgaa ccctggcttg atgtccggaa caatctgaat acctggcaaa
     1201 tcaatttctt ctttacggtc aacctgggtt cggttggagc cttgattttt ttaatggtct
     1261 cctattttgt aagggaaaaa aatctttttc aacaaaagtc cgaatccctt ttactgaata
     1321 tcctgcccaa agaaatcgtc accaaattaa agcaaggcca ccagaccatc gcagaacact
     1381 acgacagcgc gagcatcctg tttgccgatg tggtgaattt taccccggta tctgcaaccc
     1441 tgacgccttc cgaactgatc gaattgctca acgcggtttt tcaaaaattc gatgaactgg
     1501 cggataaata cggagtggaa aagatcaaaa ccatcggtga ctgttatatg gccgcggcag
     1561 gcgtccccag gcccaggaag gaccatgccg tcatcctcac cgcgatggcg gtagagatgc
     1621 gggactatat caggcaacac gattttttag gaaagaaact gcagttccgt tttggcatca
     1681 actccggccc gctgatcgcc ggcgtcattg gcagtaaaaa attcagttat gacctttggg
     1741 gcgattccgt aaacatagcc tcccggatgg aatctcacgg aaccggtggc ctcatccaga
     1801 tcacccaggc tacttatgaa ctggtgaaag atcattttac ctgtacttcc ctgggtacgg
     1861 tacaggttaa agggaaaggg gaaatgcatg tatgggaagt ggtccacaga atataaacga
     1921 tagcttccgg aatattcata tacttttgcg aggtgatcct gtttattcgc gttttttgtg
     1981 tatatctgct tgctctggcc tgcctgccat gcagtgacgg cgagcacgga catgaaatac
     2041 agaccgtaga caggggatgg agcttgtcca cagatggcca cagtaaccag gaacacgagg
     2101 attgcgaaga tctgtgttct cccttctgta tttgcagttg ttgcagtacc cattccttca
     2161 gcgttaaatt ggttcccatg ggaattaaat tcaccacaat gggacctaaa caaggattaa
     2221 tttattatca agggacggtc tcaacggatt ttctcaaagc tctactccgc cctcccataa
     2281 gtcaactggg ttaaaaagca ctccctgcca accgtacagg cttaggggat agccggttat
     2341 ttggaccaaa taacgggttt tccattattc aatattttaa tcttttgact ttcatgctta
     2401 attccatcat ccttttttcg ataaaaaata agatcatcat agccctgctg actttaacgc
     2461 ttgtcatctg gggcttgtgg agtgcatcca aactaccgat tgatgcagtg ccggacatca
     2521 ccaacaatca ggtccagatc attaccatct gtcccactct tgccggacag gaggttgaac
     2581 aatttgtcac ttatccggta gaaaaatcac ttgccaatct gccggatgtc gttgaactgc
     2641 gatccatctc tcgtttcggc ttgtcggtag ttaccgttgt ttttgaagat caggtaaata
     2701 tctggtttgc acggcagctc gtgggcgaaa aaatccgtga ggccgaagaa caaattcctc
     2761 ccggatttgg aattcccgaa ttggcgccgg tcactaccgg attgggagaa atataccaat
     2821 atgtaatcca tcccaaagaa ggcagcgcag gtaaatacga tgccatggat ttgagaacca
     2881 tgcaggattg gatcgtcgca cgccagctgt atgggacacc gggagtcgct gaagtcaata
     2941 gttttggcgg attgcttaaa caatacgaag tggctgttcg acccgatcga ttgcgggcca
     3001 tgaatacaac gatgtccgaa atcttcgctg ccctggagaa taataatgaa aacaccgggg
     3061 gggcatatat cgataaaaaa cccaatgcct atttcatccg gggtattggg ctcgtcactt
     3121 ccctggaaga tgtcaagagc atagttgtaa aaaacgcacc gaatggcgtg cccattttga
     3181 ttggcgacct ggccgctgtc cgtttcggga gcgcaattcg ttatggggcg atttcctgga
     3241 atggagagaa agaggccgtc gggggaatcg tcctgatgct caagggagct aacagtgccg
     3301 aagtggtcag cagggtaaag gataaaatgg taaagattca aaaatcactt cccgatgatg
     3361 tggtgattga gcctttcctt gaccggaccg aactggttgg cagggccatt tccacggtag
     3421 agaaaaacct catcgagggg gcactcattg taatctttgt cctggtactt tttttaggaa
     3481 acttcagagc cggcatgatc gtagccagcg caatccccct ctccatgttg tttgccctcg
     3541 gcatgatgaa tgttttcggt gtcagcgcca atctcatgtc attgggggct atagattttg
     3601 gattgatcgt ggacggggcg gtaatcattg tggaagctac gatgcaccat cttggtctgc
     3661 gaaagacggg agcagaactg acccgtgctg aaatggatgc agaagtgtac aaaagcgcct
     3721 ccaaaatccg gaacagcgcc gccttcggtg agatcatcat cctggttgtc tacatcccca
     3781 tccttactct caccggcatc gaaggcaaaa tgtttcgtcc aatggctcaa accgtaggat
     3841 ttgccatcct tggcgccctg atcctttctt taacttatat acccatgatg tctgcccagt
     3901 ttttatcaag aaaaattaac catcgaaaaa ccatagccga tcggctcatg ggctggttta
     3961 ataagattta cagtccggta ctccagggag ctatccgatt taaaggcctc ctcgtgacgg
     4021 ccacattggt cattttcgtt ttcagcatct ggttgttcag cagactcgga ggagagttta
     4081 tcccacaatt ggaagaaggt gactttgcct ttcactgcat cctgcccacc ggttcttccc
     4141 tgtctcaaag catagagacc tccatgcagg cggagcgtat cctcaggcaa tttccggaag
     4201 tcaagatggt cgtggggaaa accggttcgg cggaagtccc tacagaccca atgcctcctg
     4261 aggcatctga tatgatgatt atcctgaaac ccaaggagga gtggacaacc acccgggatt
     4321 ccaaggtcct ggcttcccgt atgctggaag cgctggaagc tattccggga attttctttg
     4381 aagcaaatca acccatccag atgcgattca atgaattgat gacgggaatc cggcaggatg
     4441 tagccattaa ggttttcgga gaaaacatcg acaccctggc ttccctcgcc ccgaaagtgg
     4501 ctgaactatt aactagtgtc aacgggatca gttcgcccca ggtggaacgc accataggct
     4561 tgcctcagat caccatccgc catgaccggg gtcgactggc taattatggg atcagtattg
     4621 aggaggtcaa ccggacgatc agcgcagctt tcgccggtga aaaggcagga gttgtatttg
     4681 aaaacgaacg gagatttgac ttggtcctgc gccttgacag cgcatatcgg acatctattg
     4741 aagatgtacg ggaactgttg gtgccgactc cttccggcag tcagattcca atctctcagg
     4801 ttgctgatgt ttcattcagg cagggccctg cacagatcag ccgggacgac ggcaaacgcc
     4861 ggattgtgat tggttttaat gtcctcggac gcgatgtgga gtcggtggta caggaaatcc
     4921 agactttgct ggaagacagg cttggccttc ccgaagggta ttattacact tttggtggca
     4981 cttttgaaaa cctggagaag gccaaagcaa gactcctcct tgccgtacct gcggccctgg
     5041 ccctgatttt tatcttgctt tatttcactt ttaattccct gaaggaggcg ggcttgatat
     5101 ttactgcaat cccgatgagc gccatcggcg gggtatttgc cctttggatg cggggaatgc
     5161 ccttctccat ctccgcgggt gttggcttta ttgcactgtt cggagttgct gtgttgaatg
     5221 ggattgttct gatagccaca tttaatcaac ttgagaaaga aggagtacat gacttgatcc
     5281 agcgggtgcg cgaagggact aaaatccgct tgcgtcccgt tttgatgacc gccctggttg
     5341 catcgctcgg atttttgccc atggcgcttt ctacaggtgc cggcgcggaa gtccaaaaac
     5401 ccctcgctac tgttgttatt ggggggttga tctccgctac ctttttgact ctggtggtct
     5461 tacccttatt gtatatgttg tttatcccgg gtcatgccgg tcgcaggatg aggatcaaac
     5521 ccgtgaccat gtcaatcctt atcatcatga gcctgccccc gatgactgct ttcgcccagg
     5581 aaaaccgtcc ggtaagcctg gatgaggctg tgagacaggc aaaggccagt aatctgattc
     5641 taaaagcagg cacttataag ataacctcac tcgaagcgct tcgacccaca gcctacgaca
     5701 tccccaagac caacctggaa atgcaactgg gtcaatacaa ttcggttcga tttgaccagg
     5761 ggtttatttt ccgacaggaa cttccgaatc cgcggaacaa acaggcgggc ctttccctgg
     5821 ttcaggccaa tatcagaagt gcagaactgg ccaaggccat tactgaaaat gatttggtat
     5881 acggggtgcg gtccgcttgg tatgaaatgg tttacctgat ggaaaaacaa agagtgatgg
     5941 aaagccagga tagtatattg actcagtttg tccaggccgc ggagctcaga ttgcgaacag
     6001 gagaaaccag tgtccttgaa cgttcaacag cgatcagcca atccggtgaa atccgcaatc
     6061 aattggccgg cgtgcttgcc gattttgaaa tcgcccggtc ccgattgcag acactcatgg
     6121 tgagtcagga atgggtggca cctccttccg gcactccttt ggagaaacgg tcttttgaat
     6181 cgacggatgc agtcgaaggt aatccggtgc tggcctggta tcagcagcag gttaaaataa
     6241 gtgaagcaaa agtaactgtc gaacaggcaa agcgggctcc tgattttacc ctgggttatc
     6301 ttaatcaatc cctcagatct ctggccggtg tggaacctgg gtacagcctg accgatcgtt
     6361 tccatgtcat tcagggcggt ttggccatat cccttttcgg caaatcgcaa aaggcgcgca
     6421 ttaaagccgc agaggtggat attgaaatgg ctcaggcgaa tgccgaagcc caatcctggg
     6481 aattaaaagg ggccctccgg caatccctgc agttgctgga acggcaacgg cgatcactgg
     6541 attatttcga acagaccgcc ctgtcttcat ctggtcaaat cctgtccgct gcacagacct
     6601 ctttccgggc gggagaaatt gattatgtag aatatgtaca ggccctcaac cggtcctatc
     6661 aggtgcggtc cggttatctc gatgctttga acagttataa ccaggccgtc atccgctgcc
     6721 aatatttaat taaccaataa ttcaaaactt ttaattcaat aatcatgaaa cataagattt
     6781 ctttcatgtc ctttgcggta atcttagctt taatcattcc ggcctgcaaa gcgaaaattc
     6841 aagatgaaca tggacattcc cacagtgatg aggctgctgc catgggggaa tccggtgaac
     6901 atcatgaaga gcataccaca tcggtaagcc ttactcaaaa gcaatctgaa agcattggcc
     6961 tacgcctggg cggcctggag cacaagatcc tcaactcggg ccttaaagcc aatggcttcc
     7021 tcaaagtgcc caatcaaaac aaggcaacca tcacttccat ttatggcggt attgtaaaaa
     7081 atatactggt gatgccgggc tcccgggtca gcgcggggca aaccgtggcg caaatcctgt
     7141 atcccccgct cattcccatg caggaagaat tccttagcct tagtccaaag attgaattgg
     7201 cgagtgcgga acttgaacgt caggtggagc ttagtaatga acaggcaggg gcttttaaga
     7261 acaaacaaaa tgctgaggcc aaccttaaaa acctgatgat acggcgcgct tcgctcgaac
     7321 aacagatcaa ccagatggga atccgtgccg atcaactggc gatcgaccat ctcgtgacta
     7381 tcctggatat caagtccccg atcagtggct gggttggaaa agtctttgtc aatattggtt
     7441 cgagtgtaga tgcatccact ccaattgccg aagtgatgga caacagaagt ctccaccttg
     7501 acctgtttgt atatgaaaaa gatcttcatg aggtgcgcca ggatcagctt attgattttg
     7561 tgctgaccaa tcagccgggc aagagttata ctgcccaggt gttttccatc ggcaatactt
     7621 ttgaaaatga aaacaaggcg atagcggtgc atgctgtggt gaaaggaaac aaacagggct
     7681 tgattgacgg catgaacgtc accgccctca tcagccttaa aaatgccacg gtgcccgcct
     7741 tgcccgatga agcatttgta aaccaggatg gggttgattt tgtcttcgtt cagaaagtcg
     7801 cgggtgcgga agtccatgat gagcatcatc atgaggaagg ggatatcgct catcagaacg
     7861 aacaccatga agacggtcat caacaggacc cgggcgaaga tggccatgaa cataaggcag
     7921 aagaaaaaac agctagaaat ggggctgaag tcctatttga aaaaattcct attaagaagg
     7981 gggttagtga tctgggctac acggaagtta ccttgttgaa ggaggtccct ttaaatgccc
     8041 ggtttgtgac aaaaggggcc tttttcttat tggccaaaat gtccaatgcg ggagaagccc
     8101 atgcgcatta agcaggccct gcaggcatca gtaaaaaaac gatgtcttcc aatttgtatt
     8161 tgtacaggga ggatctttta aaatataaac atgtaattta ttatggatca tcaacatgtg
     8221 tacgaccagg atggcagaca actttgttgt accctggagg agaaagttta tagcagagcc
     8281 ggggcaaatg cattaatcca gggaaaatcc ctcgctgaaa aacatgaagg agtccggaaa
     8341 gtttcggatg aaaaaagcca tgagcatgat acccgggccc agggattaac catttttatt
     8401 ctgcctttca tcagtttgat tttgcttttg ttgggcattg gttttgatga gggactgata
     8461 ccaaatcccg gttttttcac aggaagtacc aggttgatct ggtatgcctt atcctatttc
     8521 ccggtcggat ttccggtcct gaaagaggct ttttggtctg ttttgaaggg ggaggtattc
     8581 actgaattcc tgttgatgag cctggctacc attggggctt ttgtgatcgg cgaatacccg
     8641 gaaggcgttg cggtcatgtt gttctacagc atcggcgaaa ttctccagac catggcagtc
     8701 cggagggcac aagggaacat caaggcgctg atcgatcaac gtccggatga agtgactgtt
     8761 ttcgaggaca acaaactcct aaacaagaag gcgtccgatg tggctattgg gtcgattgtg
     8821 ctgttgaagc cgggtgacca ggtggctctc gatggagtct tatggtccga atcagcgggg
     8881 tttaatacgg ctgctctaac cggtgaaagt gccccgcgca atctacaaaa aggagagccg
     8941 gtcttggcgg gcatgatcaa tctgaacagg gtttcgcaag taaaggttaa tgcaaaatac
     9001 caggatagca agttgtcgaa gatcctagac ctggttcaac atgcgacggc ccaaaaagcg
     9061 ccaacggaat tgtttatccg gaaattcgcc aaaatataca ctccggcagt ggttttactt
     9121 gcctgcttaa ttgtcttgct tcccttgttt tttacagaat cctatcgctt tgaggaatgg
     9181 atgtaccgcg ccctggtttt cctggtgatt tcctgcccct gcgccctggt catttccatc
     9241 ccattgggat atttcggcgg aataggcgcg gcaagccgtc atggcatcct atttaaaggc
     9301 agcaactttt tagacatcat ggcttcggtg caaaacatcg tcatggacaa gaccggaacg
     9361 atgaccaaag gggtttttaa agtgcaatcc gaacaaatca tgccggggtt tgaccgggag
     9421 caggtgatgc atgcagtaaa tgcgctcgaa gcacaaagta cccatcccat agcttcggcc
     9481 attgccgatc atgcgggacc tgtgcttcca gaccaggaag taaccaaggt attggagatt
     9541 ccgggtcatg gcctctccgg agaagtcaac gggaacccgg tcttagtggg taatttcaaa
     9601 ttattaaaga agtattctgt tccttatgaa atggatcctt ccctcatcga cgataccacc
     9661 atcgccattg caatgaatga aaaatttgca ggatacctga ccatcgcgga tgagcaaaaa
     9721 gaagacgccc agaccgcggt ggatcaattg aaagccttgg gcgtaaaact gaccatgttg
     9781 agtggcgata aaacatcggt tgtaagcaag gtggccagcc gcctggggat tcaaaacgca
     9841 tttggagatt tattgccgga ggataaagtg aataagataa aagagattaa agcccggcaa
     9901 gaaagcgttg cctttgttgg ggacggggta aacgatgccc ctgtcgttgc actgagcgat
     9961 gtgggtatcg cgatgggggg cctgggcagt gatgcgaccg tagaaacggc agatgtcgtc
    10021 atccaggacg accggccgtc taaaatcccc gcagccatcc ggataggtaa gttgaccaaa
    10081 cgaattgtct ggcaaaatgt gtcccttgct tttagcgtaa agatcctggt tctgggcatt
    10141 ggcgcaggag gcttggcgac tatgtgggaa gccgtttttg ccgatgtggg tgttgcttta
    10201 atagccatct tgaatgccgt acgcattcaa cgcttgcaat tttaaaggcc gtctttaaaa
    10261 aatccttcta catccgcagg ataccgttgg gaaatctgcg ctagccctta tttcggaaaa
    10321 ctgtccgatt taagccccgg gatcaacctc aaagcgtttt tataatagac ttttcggagg
    10381 acctcatcgt ccagttgcat gccgtacatt ttccagaagg cgtggtatct tttgtggtag
    10441 ggaaaatatt cgtcccccgt ctcgagcacc cggaaatagg tgggaaattc atccactaca
    10501 taagaatctt tcccaaacag gatgcggtcc tggtatttag tgaaaaaagc tttggcatga
    10561 cggggctggc ggcccagttc cgcgatcacg gcgccgatct ccacattcat attgggcatc
    10621 tcatccaaga gttttgccag tttatccagg tcgttggggt accagcccat gtgcgcgctg
    10681 atgaaggtgg ttttccggtg tttccggatg atgttcatgt gttcggtcat gacttgttcc
    10741 cagctggctt caaaattttc ccgggtcctg ccggagcggg ttttcagttc cagccagcgt
    10801 tcgttgtttt catctgcggg tttccagaag gaagcggggt ccccggagtg gatcaatacc
    10861 gggatgccga gttcgccgca tttttcccag acgggtccga tccggggatc atcgatccgg
    10921 atccgttttc cctggctgtc cttgtcgtcc atgccctggc ttttatagat cttcaggcca
    10981 cgggctccgt ttttgacatc catttccagg gctttcaccg tattttcggc ccagtcggaa
    11041 tcatcaatgc tgcggatatt gatattggca aatacgacaa aacggcccgg catataaata
    11101 ttcgcgctgt ccaccatttt tttgaggttt tcgccggtgc ccccgctgag gttgacgatc
    11161 acgctcatat tcatggcatc catttccccg ctcagtttcg tgaaataatc cttaccgata
    11221 ttgcgccaat gatggctgtg cacatcgatg aagggaaatt tagcggctgt cagtttatgt
    11281 tcaggtacga ccagggtgga ggggggatcg tatttttcaa aatccatgat ttttgcgtcc
    11341 ccgtttccgg ccccgctctg ctgggcctgt aaagggaaaa ataagaatcc aatcaccagg
    11401 atctgaaagc cttgtttgat attcatcgga caaaaatata gctatattct caaagattac
    11461 ggaagtactt ttgcacattt taaaccatgt tcaatacgac agacgaccat gcaaatcaat
    11521 gacatctccc ggctgatcca aagccggagg gcatataccc cgaaatttta cagcgacctc
    11581 cctatcgatc cttcctggat cgaaatcatg ctggaaaatg ccaattgggc cccgacccat
    11641 aagcggaccg aaccctggcg gttccgggtc tttcattccg ctgaatcgcg gcagcgcctt
    11701 tccgatttta tcgccgaaga ttacacccgg caaacccccg cagacctgta ttcggaagtg
    11761 aagctgcggg atatggccga caaacccctg gcctcgggtg ctgtgatcgc gctctgcatg
    11821 caacgcgatc ctgcccagtc gctgccggag tgggaggaaa tcgctgccgt agcctgttcg
    11881 gttcaaaacc tttggctgtc ctgctccgcc ctgggcctgg ggggttattg ggcttcaccg
    11941 ggatttatca cccgcctggg ccccattctc gaattggccg agggagaaaa atgcctgggg
    12001 ttgttttaca tgggtcatct caaaccgggg acggtcttac cggctaccag gaagccggtc
    12061 gcggagaaaa tccggtggat gaaataagcc tttccgattt tgttatacag ccaattcctt
    12121 ctattttcgc acctttattt taaaccatta gaaattaatc gtttatggca caaacactta
    12181 tgctcctgat cccggttctt gggatccttg gacttttata tacctatctc aagagcaact
    12241 gggtggccaa tcaggatgcc ggcaccgaaa aaatgcaagc gatagccaaa gcgattgctg
    12301 aaggtgcaat ggcttttctc aaagcggaat ataaagttct ggtttggttt gtccttgctg
    12361 taggcgcact gatggcgttc ggaggttcca ccactgaaag ttcttccatc ctggtgggtt
    12421 tgtcctttat catcggtgcc ggctgttccg ttctggcagg gtatatcggc atgagggtgg
    12481 ctaccaaatc caatgtacgg accacggccg ctgcccggac cagccttacc ggtgcattaa
    12541 aagtggcttt tgccgggggg tccgtgatgg gcatgggtgt ggtgggcctt ggcgtgctcg
    12601 ggctcagcgg actctttctt ctatacagca gcatgggctg ggatatcaat aaaatcatca
    12661 cggtgatcac cggcttttct ttcggtgctt cctccatcgc cctgtttgcg agggtgggcg
    12721 ggggcatata caccaaagcc gccgatgtag gcgcagacct ggtgggcaag gtggaagcgg
    12781 ggatcccaga agaccatccg ctcaatccgg ccaccatcgc tgacaatgtg ggggataatg
    12841 tgggcgacgt cgcgggcatg ggcgccgacc tctttgaatc ctatgtcgga tccatcattg
    12901 gcactatggt attgggcgcg acttttatcg ggatcgcggg atatgaaaat accaatagtt
    12961 ttaacggcct gaatgcggtc cttcttcccc tggtgctttc cgcagtgggt atcctgacct
    13021 ccatcctggg cactttcttc gtaaatacca aagaaggggg caatccccag aaagcgttga
    13081 acaccggggt aaacctttcc agcctgatta tgctagccct cacttttgtg atcacccgct
    13141 ggatgttgcc ggaaagctgg gaattcaacg gaatcactta taccagcctc ggcgtattct
    13201 gggccgtggt cctgggattg gtttcgggag tcgccattgg ttatgtgact gaatattata
    13261 cgggcaccgg taccaaacct gtgaaatcca tcgtcaaaca aagccttacg ggttcggcta
    13321 ccaatatcat agccggtttg ggcgtgggca tgcagtccac ggccattcca accctgattc
    13381 tggctgcggc catcattggc tctcattatt tcgccgggct ctatggcatc gccattgcgg
    13441 ctgttggtat gctttcgaat accgggatac agcttgcggt ggatgcttac ggaccggtcg
    13501 cggacaacgc cggtggtatt gcggaaatga gtgaattgcc caaagaagtc cgtaaacgca
    13561 cggataccct ggacgccgtc ggaaatacca ccgcggccat aggcaagggt tttgccattg
    13621 cttccgcagc tctgaccgca ctggccctgt ttgcagccta tatgagcagt gcaaaactca
    13681 atgccatcga tatttctcat cccatggtga tgtccggatt attgctgggc gccatgctgc
    13741 ccttcttatt ttcttcgctg gctatgggtg cagtgggtaa agcggccatg gatatgatcc
    13801 aggaagtgcg caggcaattc aacgaaattc ctgccctgaa agccgcgctg ggcatcatgc
    13861 gtaaaaacga aggcaaggaa atgagcgaat ggtccgaaga cgataaacat acttttgagg
    13921 ccgcgcacgg caaagccgaa tacggcaagt gcgtggccat ttccacccag gccgccgtga
    13981 aacagatgtt gctcccgggc ctgttggccg tcattgcccc aatttttatc ggattcggtt
    14041 ttggcaagga aatgctggga gggcttttgg ccggagtgac cgtgaccgga gtattgatgg
    14101 ctattttcca atccaatgcc ggaggcgcct gggacaatgc gaagaaaatg ttcgaaggcg
    14161 gagtagagat cgaaggaaag acctattaca aaggttcaga cccccacaaa gcagcggtgg
    14221 tgggggacac ggtcggagat cccttcaaag acacttccgg cccttccctg aatatcttat
    14281 taaaactgat ttcggtgatc gccctggtga tcgctccata cttatagtca tagaagaatc
    14341 atttattgaa aaaaccggtt gacttccttc aaccggtttt tttatttccc ttggtgcgct
    14401 caattttcaa ggcccttaag cctcttcccg aatttcctac cattcctacc aatcctacta
    14461 atcctactaa tcctaccaat cctactaatc ctgccaatcc taccaatcct accaatccta
    14521 ccaatcctac caatcctact aatcctacta atcctactaa tcctattaat cctcctaatc
    14581 ctactaatcc tactaatcct actaatccta ctaatcctat aaaaaaaaag ccccgcaggt
    14641 aggcctgcgg ggcaaatgaa tatgaataaa aaaaccttaa atgttcttac agtacgatca
    14701 cgctgttttg atcggcgcca tatccgttgt attcaagacg gtcagcatgc ggttccactt
    14761 cccatacctt gccgtcaatg acgtatttga attgatattc ctggggagag ctcaggtcaa
    14821 gggttttggt aaaagaaccg tctttcagtt ttttcatgac cgcttctttg gatgatttcc
    14881 agtcattaaa actgcccagg agttcgacct tttttgcttt aagcactttc tccgcttcca
    14941 ctttgaaggt caccttgcag atgttctttg atttcagata tttttttgaa attgccatag
    15001 gagtaaaaat ttaaatttga tttctgacac aaaagtactt ggtatcacaa tatctattgg
    15061 caaataactt gttaatggcg attaaagtct gtattaatta atattacaga attgtacctc
    15121 ccatggcccg gaatcgttcc ggtaagattt tgaaaattaa aaacttacct cctgtacccc
    15181 ctgttgtttt aatcttcggc ccgggagcag ggaatcggcg gaatttaacg atttcggtcc
    15241 ttgttatagg aggatccccc ctccttcggt tttgggggag gctgcctaaa agcgggcgtt
    15301 aagaataggt taaccgatta cggtattcct aaagggataa ttacatttgt ccgcttaaac
    15361 catgatgcgc cccggaattt tcattttcat cttcctgctc gcggtttcct gccagaccgg
    15421 gccgaatgat caaaaaagct ggcaccatac ccggacctac ccggtatcca acatcaaccc
    15481 ggtgagcata tccggcgatc cttccaacct ctttgtatcc agcggggcca gaacggaaat
    15541 acataaattt aattatgacg ggggcctgat cgaagccatc aagaacatcc gcaaacccaa
    15601 gtatatcaac atcctcaatc ctggggaatt gttggtcgcc gaaagctccg cccacgtggc
    15661 cacccgggtc gtcaaccaaa acagcatcca caccatcccc accaacgaat acctggatac
    15721 gccctatgga gtgagcggcg ccggtaattc cctggtcatc acggactatt ataaaaacaa
    15781 ggtctattat aacaaggggg ggcagatcct gagctttgga aggccgggaa tcggcgaagg
    15841 cgaattcaat ggtccgacgg atatccagct ggccaacaac aaaatctacg tctgcgattc
    15901 ccggaataac cgggtgcagg tctttgatga aaacggcaaa tacctactgg ctgtcggaga
    15961 agaagaccag atcaaaaccg cctccggact gtttgtgacc ccggatgaaa tcatcgtcac
    16021 cgattatacc ggcaaccgcg tgctgatcta tgaccagaaa ggcaaattga aacaaagcct
    16081 gaccgaaccc tttgaccagc cttcggatgt ttttgtccgg ggcagggaaa tgtttgtggt
    16141 caactattac gccaactcca tagaagtttt tacgaagtac tgatcccgga ttcccggtta
    16201 cagtccttgt ttaccctcat gctttcaggc atgttcttcc tcgtattccc gggcgtattc
    16261 ctcggtcagt ttacgctgaa gttctgcagg gaccggtccg tattccgcaa attttaatga
    16321 aaacttggcc ctgccctggg tgagcgatct cagggtcgag ccatagttat caatctcagc
    16381 caggggcacc cgggctttta ttttctgata gtggccgtcg cggtccatac ccatgatgat
    16441 ggcgcgtcgc gtctgaaggt cacccatgat atcccccatc gaggtatcgg aacacaggat
    16501 ttccagatca tagatgggtt ccagcaactg cggtcccgca ttctgaaaat tgtttttgaa
    16561 ggccatggtc ccggcaatct ggaaagccat gtcgttgctg tccacggggt gcattttacc
    16621 gtcgtagata cagacccgga tatccctgca ccggctgccg gtgagcgggc cttccgacat
    16681 tttattcaac actccctttt taatggcatt ggaaaacttt gcgtcgatgg acccgccgac
    16741 gatacaccag taaaatacca gtttgccgcc ccagggcata atgtcctcct cccggttgcg
    16801 gaccgtgagg tcggaaggat cgggcatgcc ttcataatag ggttcgatgc gcatatgcac
    16861 ttccgcaaac tgtccggccc ctccggattg ttttttatgg cggtaatcgc cgttggccac
    16921 ccgggtgatg gtttcccggt atgggatcct gggtttgaca aaatccatgc ggatgtcctg
    16981 ccccttctcc acccgctgcc tgaccatatc caggtgcaat tgtccctggc cgtacagcaa
    17041 catttgttta agttcggcgg actgttccac gatcacggtg ggatcttcct cctggatgat
    17101 gtgtagggcc ttggccagtt tttccacatc gtttttggat gggggcacca ccgccacccg
    17161 gatgcggggt tccggaaaat ggatgggttc gattttccgg tccaggcctt taacgttcag
    17221 ggtattattg gtatgggaat ccttgagttt gaccaccact ccgatatccc cggcataaag
    17281 cgtatcgacc gactcccggt ttttgccgtt ggcaatgtac aactggccaa accgttcgga
    17341 gttgcggttg cttgcattga cgagttcatc gccggttttc agcttgccgg cataaacctt
    17401 aaaataagag acattgccca ctttgggttc ggtgatggtc ttatagatga agatggtggt
    17461 ctggtcctcg ctgttgcagg gcaggttgcc tccgccttcc agcggtgcgg cgggacggtc
    17521 ggcaggagaa gggcagatat cgtggacaaa tcccatgatc cttccggtgc ccatattgtt
    17581 cctggctgag gcgcagaaca cggggaagag gctttgatgt gcaatggcca tccgcaggcc
    17641 ataggccagg tcttcttcgc tcagggtacc ctgttcaaaa aaccgggtca tcagttcctc
    17701 ctcgttttcc gcagcggcct ccaccagttt attatgcaat tccagcgcct tctctttttc
    17761 cgattccggg atatcttttt ttatgggttt gcccccgtcg ggagggaatt catagacgac
    17821 catgcgcagg gcgtccacga tggaatgaaa tccggcgccc tggttgagcg ggtattggat
    17881 ggggactacc tttttaccaa agcgggagac ggcctgttcc agggtgttgt caaaatccgc
    17941 tttctcgtga tccagttggt tgatgacaaa gagggagggg gtgttaaatt tttcgacgta
    18001 ttcccacatg agttcggtac ccacttccac gccatgggcc gcattgagta ccatcatcgc
    18061 tgtatcggcc accttgaggg cggagatgac ttcaccgata aaatcatccg agcccggcgt
    18121 atcgatgatg ttgatttttg atcctttcca gatcgtatgt tccagtgaac tgaacaagct
    18181 gcttttccgt tcctgttcga gcggattata gtcggatacg gtattccctt cctccacatt
    18241 tcccctgcgg gtaatgccat gcgcctcata cagcatacac tcaatcatgg tcgtttttcc
    18301 ggagtgcgca tggccaagca agaccacgtt gcgcaagtct tttgtctcga agttcataat
    18361 acaatacgtt ttattgattt ccttaaaatg tcgagctgga tcgcctcaaa tcctgttgga
    18421 attgaaaccc agttactgcc ttcaatttaa ttcaaaaggg cagataaaac aaacccattt
    18481 ccaataaaat gggaatccat cgccgcactc cctgtaaaca attcatcctt gtccttaaat
    18541 acattaaata aaaatggata tgttgacgaa ggcccggttt ccaagttctc aggccttgag
    18601 gtagatgccc tttctgtcca ggtaataacc gaataaccaa caaaccaaca taaaactgat
    18661 ggcaaataaa aaggacccgg aataattgcc tagaggctgg aataccgact cataaatgga
    18721 agaataaaaa ctttttccag atcccgtctg aaaaaaccag agcaggatag ccagcaattc
    18781 ggagaatagg taaacggcca gcggattccg ccccggtacc aggaaaaaac gggtccatcc
    18841 tttccatgcg atcttatcca ccgcatagac caagaggctc agaaggatca tgtcccagcc
    18901 gaccgtttgc aatacaaaag aactggtcca cagttttttg ttgatcggaa aaacctggtg
    18961 ccaggcgatg gaaaccagga tcaacaccac tcccgcggca aacaggcgca ccagctggga
    19021 gggcttgcgg ccaatctctt ccaggtagac ggtaaccagg aaaccaaaaa ggacgttgac
    19081 cagggaaggc agcgtactca ggatcccttc tggctcaaag gctactccct ccccgtgata
    19141 catatgtttc tcccccagga tggccaggtc aatcttctga cccaggttgc ccagcatggt
    19201 atagtcccct ccccaggcca tcagacccca ataagccagc aaaaggccca ggctgatccc
    19261 tacgattttt ttccagtcca gcttgaaaac catcagggcg gccaccccat aacacaaagc
    19321 gattctctgg agcactccca ggatgcgcgt gtcgctgaaa ggactgaact ccaaaccccc
    19381 ttcggcccgc cggacgaagg gaaaccagta catcaggaat cccagcagga agatgataaa
    19441 ggttcttttg aggatccgcc ggtataccgc agccggtgcg gcatgctccc aggaccgccg
    19501 gctgaaataa agcgaactgc ccactgcgaa taaaaaggaa gggaagacca ggtcggtggg
    19561 ggtaaatcca tgccaggatg cgtgttgcag gggggcatag gtatattccc aggtgcccgg
    19621 ggtattgacg atgatcataa aacaaacggt cagtccccgg aatacatcga gtgccaggtt
    19681 gcgggaatgg atattcatgg gtggaattta cgaattagga ataattcata agccaatact
    19741 ctcccggttt ttccatgaag caacctttcc cggaacaaaa ggtctttgtt aaccggaaca
    19801 gatcaaatca tgaaatcgtt tttattcctt gccctctttt tcctgttcaa gccggtgttt
    19861 tccaagggac aggattttga gatcccggag gacacggcca ggctggacat tggaaaactg
    19921 gccaggcaca ttcttcccga ttccacggca accacctacg ccagggtgtc cgcggtcgtg
    19981 agctggacca atacccggtt tgcatggacc tatacggatt ataaacgccg caccgtcaag
    20041 gagatcatct gccggcgggg cggcaactgc aatgaacagg caagggtagt gagcgccctg
    20101 ctgacggaag cgggaatcca aaacaggagg gtttctgaaa tcaatatcca gcctgaatca
    20161 acccaaaggc aggagagtgc agaaaaaaaa atcaaagaaa acggattccg gatgtccgta
    20221 tttggtttaa gacataacga tcatgtatgg atcgaatatt tcgatgaaaa tctgaaggca
    20281 tgggaacccg ctgaccctac cctcggcctg gtcggcctgg aggcctggat caaagccagg
    20341 gctggatttg ggcaacggat caaccatgat attctgcctt cgagggatat gctggtcccc
    20401 attgcggttt tcgccctggg tccggatggg accatcctgg aaaaccgcag ccggcactat
    20461 ctggtaaatg cttttaacca agtttatgaa aaaaaactct ccgccggtcc ggcctggtcc
    20521 ggatggacgg aggaagtgct taatctggaa caggtatgcc ggcaagcctt tgaaggcagg
    20581 gtcaatctgc acgagcatac cgatcaaata aaaaagatcc gggaagaata tcaatccctg
    20641 cgcacctggt atattgaccg gcatgaaccc tgaatccctt tttactccgg tctcaaaccg
    20701 gggctgatga tcctgggcac ttttcattgc cgggatgcgg ggaaggaggt tacaaacccc
    20761 ggtttgcctt tgaggtctta tccgccggga ggcagaaaga agtagtggac ctggtcaatg
    20821 ctcttgctct gtacaagccc actaaaattg ccgtggaatg gataaaagag gaagaccagg
    20881 ctttcacgga ttctttatac caggaatacc tggaaggccg gcgtgacggg gtattttagg
    20941 gaatgattcc gttcagcctt tgtcttttat ggactctttt tttatttatc ctcaaggtcc
    21001 gattttccgg taattgccgg aatcaaacct cggcgcatag acacagagaa aaattaaatc
    21061 ttcaccggcg atgtttttaa tcctttggga tgaattccgg ggaataaaaa ccaggtcccc
    21121 cgcgcccacc tcaccaatga tccggccgtc gatctccacc tccccggtac ccgacagaat
    21181 ataataaacc tcatcggtgt cctgcacgct gtgcagggcg gtcgtgaccc ccggttcaac
    21241 ccgcgcccgg gccaaagaga ataacccata ttccttttgg ttgaagatct cgtcaataaa
    21301 acatttttct tccgtaaaaa tttctttatg cccgcttgcc cggatgattc ttttcatatt
    21361 gccttgtcaa accaatgtat aaaaatgcgg atgactttta tcagtttttt ctcctccggg
    21421 gacatattcc ctctaacttt cactttgaaa taatattcag ccttgtgaaa gaaatcaaac
    21481 agatcattaa acaatatgaa tcagccaggg agaaaggaat ccggaccgcc ctggcgaccg
    21541 tggtgcacct ggaaggctcc tcctaccgca ggcccggagc aaggatgctg atcgaagaaa
    21601 acggtcaact gaccggcgcc atcagcggag gttgcctcga aggcgatgcc ctgcgcaaag
    21661 ccctgcatgt catcgcggag aggagggcca tgctggtcac ttacgatacc atggatgagg
    21721 acgacgccaa attcggggta gggctcgggt gcaacggcat cattcaggtt ctgatcgaac
    21781 cgatccttga tcaggacccg ctcaacccgg tgcaattgct aaaaatcctg gccgggtcaa
    21841 gggaacctgc cgttttgctg accttattcg acctttcgga ccgcaaagcc gtccaacacg
    21901 ggacctgcct gatttaccat actccccata aagtcatggg aaccgtgccg gttttttcag
    21961 accggatccg ggaagacatt gactccgccc tccttcaaaa atcatccctt ttccgacaat
    22021 atggggacga taacgtccag gtcaccgcat tcatcgaata cgtggatcct ccggtggccc
    22081 tcatcgtcgt aggcgcgggc aacgatgtca tgccgctggt ggacatggct aatatcctgg
    22141 gttgggaacc ccatgtggtg gatggaaggg ccaattacgc caccacagac cgctttggtt
    22201 ctgcctgcag catcatcgtg gctaaaccgg aaaaagtctt ggaacaaata aggatcgatc
    22261 accgcacggt ctttgtcctc atgacccaca attacaatta tgacctagcc atgctccgtg
    22321 ccttactgga tgtggagacg ccatacattg gctccctggg cccccgtaaa aaactggacc
    22381 ggatgctcga cgaactccgg gaggaaggaa tccagatcac ggaccagcaa aggcaaaaaa
    22441 tattcggacc ggttggactg gacctgggtg ctgaaacctc agaagaaatc gccctctcca
    22501 tcctggccga aatcgaggcc gtgcttcacg gaaccactgg gggccagctc agggacgcag
    22561 gggaatatat tcattcgagg gaatctttga agattattca atagcagtag tcagtagtca
    22621 atagtcagta gtcagtagtc agtagtcagt agtcaatagt cagtctcgca tctcgtgtct
    22681 cgtatctcgt gtctcgcatc tcgcatctcg tgtctcgcat ctcgcatctc gcatctactt
    22741 taaaccaccc gtttgactaa ggcaaaaaaa atcgggattg taatccttgc ggccggggct
    22801 tccaggagac taggctcagc caaacagttg ttgaaggcaa acgacactca aaccttattg
    22861 aatcatgctg cgaccatagc cctgtcgacg ctcttccggc ctgtggtagt agtcctgggc
    22921 tcaaatcatg aagccctggc caacgaactt tccggcctga atctgttcct ggccatcaat
    22981 gcacaatggc aaagcggtct ggcttcttcg gtgaaatgcg gccttcggga agtcctgaac
    23041 ttgcagggcg atacggatgc cctgatcttc atggtctgtg accagcctta tgtgactggc
    23101 cccttgctgg aagcgctggt caccacctat caggcttccg ggaaaaacat cgtggccagc
    23161 aggtataaag aagccaacgg cagccccgcg ctttttgacc gccgtttttt cccagaactg
    23221 gaaaagctgg agggggataa aggagccctg agactgatcc atcaataccc ggaccaggca
    23281 ggttttgtcc cttttccaaa agggcaaatt gacatcgaca cggtggaaga ctacctcagg
    23341 tataaaaacc aggaaaaata aaccgcaggg ccgaacctga cttgtcctat tcaatacccg
    23401 gctctttatt ttaaattgac gggcgagtca aggatattcc aaaaaaaata ccaaataaaa
    23461 ttggatcagc gggatattat attctgataa aaacttgatt tcatggaccg ggccctttaa
    23521 attgggggtc tattgtactt ttcaaaaaca gggaaatcaa ggatgtcgtg ccaatcatgc
    23581 cggaaagaag ggaagaaacg aaaatccgtg tccctatggt ccggcctctt cctgatcctc
    23641 cttccaaaat gtcccttttg tgtgattgcc tacagcggta ccatagccct ttgcggaaat
    23701 ggcccgtcgg cagcccccaa tgcccatgaa aaccagctgg cccttgcgct ttcggtcatg
    23761 ctcagcctca ttattgctgc agccatcgcc ctgaattacc gcgggtggcg cacctggatg
    23821 gcgctgggac tggctttcac cggcatgctg gtcctcaacg gatcgatcct gaaaccggcc
    23881 ggatatttct ggtattatac gggagtggtc atcctttttt caggagtctg gctcaacgga
    23941 agcctcctgt atttgatcaa ccgccgccgg cctttagcca gggcgggacg gaccctcgac
    24001 tatttacaaa acattaaata acgattcgag tatgattgca caaccttttc agtatgaagc
    24061 tccggcaacc ctgcaagacg ccatcggcct cttgcagaaa tacggggatg aagccaaaat
    24121 actggccggc ggacacagcc tgatccccat gatgaaactg cggtttgcca caccgggcta
    24181 cctcatcgac atcaataata ttcccggcct ctcctcgatc caggaagaaa atggatttat
    24241 ccggatcggc gccctggtcc gcgaagcgga ggtggaacac tcggccctgc tgaccgggca
    24301 ctaccccatt ttcgcggatt cgacccggct gattgctgac ccccaggtgc gcaacatggg
    24361 caccatcggg ggcaatctcg cgcatggaga tgccgccaac gaccatcccg cggtgatgct
    24421 tgcactgagg gccagggtgg ttgcaacggg cccaaagggg agccggacca tagccattga
    24481 cgattttttc cagggttttt ataccaccgc cctggagccg gaagagatcc tcacggagat
    24541 ccaaatcccg atacctcccg ggcataccgg cagcgcctac tataaactcg aaagaaaggt
    24601 gggggattat gccatcgccg gggtggccgt ggtcctgacc ctcgatggaa atctggtgaa
    24661 atccattggc ataggactta ccaacgtcaa cccggtgccc atgcgggcca gccgctccga
    24721 agagttcctg ctgggcaaac cgctgaccga cgaaaaccta gcccaggcag gcaaactggc
    24781 cgctgaagac tgcagccctt ccaccgacct gagaggcagt gaagaataca aaagggacat
    24841 ggtcgccgtg gtattaagac gaatggtaaa actggccgcc gaaagggcca aataattttt
    24901 taatcttctg aattcaaagc atatgaaaat aaatgtcacc gtcaatggaa ccgcccatga
    24961 agttgaagtc gaagcccgca ctttactggt gcatatgatc cgggaaatcc tcaacctgac
    25021 cgggacccat attggatgcg acacctccag ctgcggggcc tgcaccatcc tgctcaacgg
    25081 caagtcggtc aaatcatgca ccgttcttgc ggtccaggcc aatggtaagt ccattaccac
    25141 catagaaggt ttggctacgg ccgaagggct tcacccgctc caggaaggat tcaaggaaaa
    25201 tcacggactg cattgcggtt tctgcacccc gggcatgatc ctccgcgcaa gcgaattgct
    25261 agccaataac cccaacccca cggaagaaca gatccgctgg ggtatttcgg gcaatctttg
    25321 ccgctgcacg ggctataaca atattgtcaa atccattcag tatgccgcgg caaaaatgag
    25381 gggggacgcc ctgccttcca cagaacccgt attcgtttaa tccaattctt taatctgaaa
    25441 atcaatatac aatcatgatt cagtgtaaaa cttcaaaggc catcggcggc atggggcatt
    25501 ccctgaaacg caaagaagat gtccgtttta tccagggagc gggccattac gtggacgata
    25561 tcaaactgga cggcatgctc tatatggata ttgtacgcag cccttacgct tatgccaaaa
    25621 taaaaaacat agacatctcc gaagcgatga aagtgcccgg ggtagccgcg gtggtgaccg
    25681 gcaaagacct cgaaaaatac aacctgcact ggatgccgac cctgatgtcc gacacccaga
    25741 tggtccttcc caccgatacg gtcatgtacc aggcccagga ggtggcggcg gtgatcgcca
    25801 ccagccgtta tgccgccagg gacgggcggg aagccgtaaa agtggaatac gaacccatga
    25861 aaccgatcat ggatccctac aaatccatgg acccatcctc ccccgtcctg cgcaccgaca
    25921 aacccggtaa gtcagacaac catatctggc actgggaagt gggtgacaaa gccaaaaccg
    25981 atgaggtatt caaaaaagcc gaggtcgtgg tcgaacaaca tatgcatatc ccaaggatcc
    26041 acgtggcttc catcgaaacc tgcggttgtg tggcggacta tgacaaaatc aacaaccacc
    26101 tcaccatgta tatgaccacc caggctcccc acgcgatccg caccgtgatc gccctggtag
    26161 ccgggcatgt gggcctcacg gaggaaaaga tccgcgtggt ctccccggat atcgggggag
    26221 gatttggcgg aaaagtaccg gtctatccgg gttatgtcat cgccatagcg gtgtccttcc
    26281 tcacgggcaa accggtaaaa tggatcgaag accgcagtga aaacctgcag gccgactcct
    26341 ttgcccggga ctatcacatc acagtggaaa tggcgggcac caaggccgga aagatccagg
    26401 ccacccgttt taaagtcctt gccgaccatg gatacaccga tgcctcggcc aatccttcca
    26461 aattccacac cggcctgttt tccatcggta cggggtctta cgactacgac accgctttta
    26521 tggaagccga tgccgtttat accaacaaac ccccgggcgg ggtagcctac cgctgttctt
    26581 tccgggtcac cgaagccgtg caggccatcg aaaggattac cgatctgttt gccctcaagg
    26641 taggcaaaga cccggcccag ctccgcatgg aaaactttat caaaaaggag gatttcccct
    26701 ggaaatctcc aaccgggtgg acctatgaca gcggggatta ccatgccggc ctgaaaaaag
    26761 ccatggaggc catcggctat gattccttgc ggaaggaaca ggccgaaaaa agggccaaag
    26821 gtgaattgat ggggataggg atcagcacct ttaccgaaat tgtgggtgcc gggccctcca
    26881 aagattttga cattttgggc attaaaatgt ttgacagcgc ggaaatccgc gtgcatccga
    26941 ccggcaaggc cattgcccgc ttcgggacga aatcccaagg ccagggccac gaaaccacct
    27001 atgcgcagat catcgcggaa gaactgggca tccctgccga aaatatccat gtagaggagg
    27061 gtgataccga taccgcccct tacgggctgg gcacctatgc cagccgcagc acgccaacgg
    27121 ccggagccgc cgcggcagta gcggcccgaa aactccggga caaggccagg aaaattgccg
    27181 cttacctgct cgaagtcagc gaagaagacc tggaatggga acccggaaaa ttctctgtaa
    27241 aaggagctcc ggaaaaagca aaaaccatcc aggaaatcgt gtttgcagct tataccaacc
    27301 atccgcaggg catggaagcc ggttttgaag cgactcatta ttacgatccg cccaacctga
    27361 cctttccatc cggcgcttac atctgcgtag tagacatcga caaggaaacg gggcagatca
    27421 aagtgcgccg ttttgtcgcc attgacgatt gcggccatat catcaacccg atgatcgtac
    27481 agggacaggt ccatggaggc cttacccagg gcatcgcccc ggccatgtat gaagaactga
    27541 tctatgatga agacggcaat atcctcaacg gcaccttcat ggattacctg gtgcccactg
    27601 cggtggaatc cccgcattgg gaaaccgggc atgtggtgac tccttccccc caccacccca
    27661 ttggggccaa gggagtcggt gaatcgccca ccgtgggcgc gcccccggcc atagccaatg
    27721 ccatcatgga tgcgctctcg ccttatggca tccaccacat cgatatcccc atcaccccgt
    27781 ttaaggtgtg gaaagccctg aaggagaagg gcgtgatttg accattgaaa aaagaccagg
    27841 ggtaagtttt ccttaaaatg gaaaactacc cctgttttat tttgttatcc ttaaaagaga
    27901 aattatgaat gcaaaaatca ataaaaccat taagctggat cacccggtgg atgcggtatg
    27961 ggaactcatt tccgatccgt acaaggtagt ctcctgcgtc cccggcgcct ccctgacctc
    28021 ccaggaaagc gaaaacgatt ataaaggcca ggtggagttt aaacttggcc cggtgaaatc
    28081 caaattcgac gggaaggtta cctttttatc gaaggatccg aacttgaaaa aaatggccct
    28141 gaaaggcacc ggcctcgaca gcaaggggaa aggaagcgca gagatgagca tggatgccga
    28201 agtgaaagcc ggaaccacgg ggacggaagt aagcctgaat atggatgtca gcatcaccgg
    28261 catgctggct cagtttggtt cccgcctcat caatgatgtt tcgagccagt tgctcgatca
    28321 gtttgcggac aatctgaaag ccaaattgtc ggggggagag gtggatacca gcatccatac
    28381 gggttccatt gtgggcagtg tagtgaaagg attgttcgga ggaaagagtt aagcctgcct
    28441 gttggaacgg tttcatccgg catccccgga atctatcata aaagccctct cggacctggg
    28501 atatgtagca gaggaagacc ttgccatagt cttgtttttg gtcctcacgc ttcgcaaacc
    28561 gcttttgctg gagggcccgc ccggtgtggg caagacggag gtcgggcttg ccctttccgg
    28621 tttattgaag gcacccctga tccggctcca gtgttacgaa ggcctcgacc tcaaccaggc
    28681 cgtatacgaa tggaattacc agaaacaatt gctcagcatc aaactgctgg aatcctcggg
    28741 ctccagcctc agggacaagg aagaccttat cttttcggag gagtacatcc tcaaaaggcc
    28801 cttgctgcag gccatcacgc aggcagacca atcccccgta ttgctcatcg atgaaatcga
    28861 ccgggcggat gaggaatttg aagccttcct gctggagtta ttgtccgctt ttcaactgag
    28921 cattccggaa atcgggacgg tgaaagccac ccataaaccg tatgtgatcc tgacttccaa
    28981 ccggacgcgc gaactgagcg acgccctgaa acggcgatgc ctctatcact ggatcgactt
    29041 cccggatttt gacaaagaaa tgcggatcgt ggagaaaaaa cttccgggca ttgaagaaaa
    29101 attactgaag caggtcgtgg gttttgtcca gaaactaagg tcccgggaac tggccaaaca
    29161 acccggtatt tcagaaacgc tggactgggc cgagtccctg atgaccctgg gttaccgcgc
    29221 ccttgatcaa aagacgctgg atcataccct gggcataata ttaaaatcga acgaggacat
    29281 caaaatgatc cgcgaagaag gcatcgaatc cctggtttga gccatgtata cccaagccta
    29341 ttccgctcgc agcatatcct ccggtatggt cggatttttc cgctacctga ggtcccatgg
    29401 atttttgatt ggattgcggg agaccacgga agcacttgaa gtcgcccaca cctcgttggt
    29461 ccgggacaag catcgtttaa aaacagccct ggctgctctt tgttgcacat ccagggaaga
    29521 actcgccctt tttgaccgct tgtttgcagc ctattgggat accagcccca tggacctggc
    29581 agagcggaaa aaccaagtca ccgtgcgggg gcaggtccga agtaaaatca atacttccct
    29641 ggtactgctc ggccgcggca aaggcaccgg gaaagaagag gaggaagcca ccagcgtatc
    29701 tggagccaat gcagaggaac gtttgcgcga aaccgatttt tccaagatcc gggagatcga
    29761 tgaaaaaccc ctggagcaac tggccgaacg gctgttcagg gaatttgcgg tgaaaatgcg
    29821 acgtaaactg aaagccacca ggaagcccgg caaggtccac ctggccagga cggtccgcaa
    29881 gagcattgaa agcggaggag atcccctgaa gcttttctac accacccgca aactgaaaaa
    29941 aagaaggctc gtgatcctcc tggatgtgag cggatccatg gacaagtaca gtttttacct
    30001 tctccggttt atctatgcgc tgaaacagga attcagcaac ctggaggcat ttgtattcag
    30061 cacccgcttg atatccatca ccagggccct ccgcaatatg aaattggatt ccgtgctccg
    30121 ggaggtctcc cattttgcca gtcactggtc gggcggtacc cgcatcgggg aatgcctccg
    30181 ggacttcaac cgccaatatt ccaaatatgt cctgcatgga tcacccgctg taatcatcct
    30241 gagcgatggc ctggaaaccg gaaatccggt acttttaggg tccgagatga tcaagattcg
    30301 caggaggagc aaggtgctga tctggctcaa tccgctgaaa ggaatgaaag actatcaacc
    30361 cctggccgga ggcatgaagg ccgccctgcc ttcggtggac gaattccgcg cagcgcataa
    30421 cctgaaaagt ttacttgaac tcgaagattt attgacccat gtctgacgat tacctgaata
    30481 aactgatcga actgagcaaa aggaacgaag cctttgcctc ggtgaccgtg gtgcgccgcg
    30541 aggaaccctc ctccggcaag gccggggata aagccatcgt caaccaacag ggggaacttt
    30601 ttggctggat cggcggcggc tgcgtcaaat cgatcgtctg ctcggaagcc atggatgcca
    30661 tcaacaccgg aaaaccccgg ctggtgaaaa taggccagga aggtactttg attcagcagg
    30721 atgattttaa aagctataaa atgacctgtc acagtgaagg ctccgtggag gtgttcatcg
    30781 aaccctggct gcaatccccc cacctcatcg tcatcggaaa aaccgcgatt gcgaaagccc
    30841 tggtcagact ggcgaaggcc gccggctacc gggtgaccgc cgtggccccc gaggccggcc
    30901 cccagacttt tgaaaaagtg gatgagctca tcacccagct caacctttcc ggagtgcaaa
    30961 gcagcccgag gtcatccatc ctgatcgcca cccaggggga atcggatgag acggccctgg
    31021 aaatggcttt gcaaaaagaa gccggttata taggctttgt agcgagccgg aagaaaaagg
    31081 aagcggtctg gaaattcctg aaagatcaat ttatccctga tgcccagctc gagcgggtat
    31141 cctgtcccgc gggacttgat atccgggcca aaagccctga ggaagtggcc atcagcatcc
    31201 tggcccagat catccaggtc caggccaacg caccggtctc cccgctaaaa cccgaacaga
    31261 aaaaggagga aaaacaatat tatatcaacc cggtttgcgg cattcccgtg gatatccacc
    31321 accccaaaca catcctcgaa taccagggtg aaaaagtcta tttctgctgc gacggctgca
    31381 aggtccagtt tgaaaaggaa ccggaaaaat acctgcccaa aagcatagca tgatccagga
    31441 caaacacgga cgcgtacata cctatctccg gatttccctc accgacaatt gcaacctgag
    31501 gtgtttttat tgcatgcctg aagaggatta cgaattcacc cccgcctccc ggctcatgca
    31561 ggccgcggaa atcgaaaccc tggccaggac ctttgtggat ctcggcgtca acaagatccg
    31621 gttgaccggc ggggaacccc tggtccgcaa ggatgccgcc gacatcatcc tccgcctctc
    31681 ccggcttccc gtcaaactga cgctgaccac caacgccacc cgggtccatg agttcatcga
    31741 tgtgtttaaa gaggccggga tccgttccct caacgtcagc ctggataccc tcgaccggga
    31801 ccgctttctt tttatgaccc gccgggacca gtttcagcgg actcttgata atatccgtct
    31861 cctggtcgac cagggcttcc atgtgaagat caatatggtc gtcatgaaag ggctcaacga
    31921 atccgagatc cccgattttg tagcctggac caaaaaggag cccatccatg tgcgcttcat
    31981 tgaattcatg ccttttgaag gcaaccgctg gacgagcaac aaagtattca ccctgcagga
    32041 aatgctggaa ctgataggca gtcgattcga aattgaggcc ctgctcaacg ggatccacga
    32101 caccgacaaa aaattcaggg ccctgggcca tgcaggcacc tttgccgtca tcagcaccat
    32161 gagctccccc ttctgcgccg gttgcaaccg gatccggctc accgccgacg gtaaaataaa
    32221 aaactgcctg ttttcaaaaa ccgaaaccga cctgctgacg gccctgcgca agggagaaga
    32281 tgtggtccgt ctcattcacc agaatattgg gcaaaaagcc aaagaactgg gagggcagtt
    32341 tgatgcggat atcgagcata tccatccgga agagatccat aaccggagta tgattacgat
    32401 tgggggataa atatatatta agtaataatt tattatttta aataatttat atcatgacca
    32461 cagaagagga tcaaaaaaat aatgagaaaa taaaacaaga agaatcatct cataatttcg
    32521 ctaaacctgc agatacttcg aatccaacag acccattctc cctaccaata gaatatttgc
    32581 caaaaactcc tttatggcct ggcaacaatc tcgacagtca caaaaaaata ataaactttt
    32641 ctgactcttt aaaacactct ttaaagtcac tatcagaagt tcttccaaaa ataacgccta
    32701 atataacttc tagcttaata cccaaaatta cacctagcat aatgcctact cttactccaa
    32761 ctctaaattt attatttaaa catcaatctg aaataagtga tcttgaatca gaaataaaaa
    32821 atattttaat aaaaaatgaa caactacaag gtgaagcatc attaaaggat gaaaagatta
    32881 atgaattaaa taaaaaggta ttagaattaa gtcataaaca aaaattaggc tatcttttat
    32941 cccaagtaaa tgattttgga caaaaaatat tattggaatc agaagaattt agaaacaaat
    33001 tttataataa ggaaaatcaa aaagcttttg tggtttcaat tgatttgaga aggtcaactg
    33061 aattaatgct aaaagccaag tccccagaac attttgaaaa attcatttca gaattatgtg
    33121 aatccctttc tgaaataata aaagagaatt ttggaatatt cgataaattt acaggcgatg
    33181 gcattttagc ctactttcca ttattttaca gtggagagga tgctggttta tttgccctaa
    33241 aagcaagtat agactctcat aagatttttt caaatcatta tgaaaaacat agatcaagtt
    33301 ttcaagcagt attaaaaaat gctggcttag gtataggaat tgattttggt gttaccaaat
    33361 tagtgaacct tattaattct cacactgtca taggtgttcc agttgtttat gcatgcagga
    33421 tgtctagtca ctacgcaggt tcgacagtat taaatcaatc agcttacgat gaattaaaga
    33481 aacaaaactc aattggattg aaatttacag aaataacatt accagttaaa aatgaagacg
    33541 attttattgg ctatttggtg gaatcaacag gagatgatat caaaccaaaa caaccattgt
    33601 ggttgaaaga aataaaggag gattgagttt aacagcctgc aaagctatag ctttcgaaaa
    33661 tttccatggt ttctaatgct cgtaatagaa tgtaacttct ctcttttagc ctctgtactc
    33721 taaactctga cttctaaccc taatatgaat ttcatttccg ttcaggaagc aaaattccta
    33781 atcctcaacc aggttgacca tcccaaaccc gtagccgtcc acctggatca ggcgaccggc
    33841 aaaatcctgg ctgaagatat cctctccccc ctcgacctcc cacccttccc ccaatcctcg
    33901 atggacggtt atgcttttcg ggcggatagt tacgatccgg gaaaggggct gctgatcacc
    33961 gaagtaatac cggcaggcca gggcagggaa atcctgatcg gaaagggaga ggcggtgcgc
    34021 atcttcaccg gggcggcggt accccagggt gccgataccg tgatcatgca ggagaaaacc
    34081 gaagtcaggg atggccgatt gtatctaaag gaggaagtcc ctcctccagg gacccatgtc
    34141 cgtcccgctg gctccgagat ccggaagggc gagcaagcct tgcccacagg ccatctgctg
    34201 aacccggctt ccatcggatt gctggccagt atgggtctca cttccgtagc cgttcatacc
    34261 cctccttccg tacatatcct ggtcaccggg gatgaactga cctcaccggg tgagcccctt
    34321 ccttacggca gggtattcga ctccaattcc tttaccctaa gagctgcctt tcagcttgca
    34381 ggaattgaag atatccggat ctcccaggtc cctgaccggg aagacctgct ctctgaggct
    34441 atctcttcag ccctcgaaaa gcaggacctg gtcgtattga ccgggggggt gagcgtaggc
    34501 gactttgatt ttgtagccgc gacggccatg cgctgcggag tggaaaaagt cttccataag
    34561 atcaaacaaa agccgggaaa accaatattc tttgggatca aaggaaacaa actggtgttc
    34621 gggctccccg gtaatcccgc ttccgccctc acctgttttt acgaatacct cgaacctgcc
    34681 gtgcaaaaac tcttgggcaa gtccagcgcc gtccgtaagg tcaacgcccc gatccagtcc
    34741 tcttataaaa aagcccccgg cctcactcat ttcctcaagg cctggtacga cggatcccgg
    34801 gtattccccc tggacgccca ggaatctttc cgcctccgtt ccttcgcacg ggccaatgcc
    34861 ctcatcgtga tcgacgaaaa ggacgacggg gtgcaagccg gcgaacaggt tgaagtccat
    34921 atcttgccgg tataatcagt tccggcatgg attacgcatg gtggttttac ctcattctgt
    34981 atgtggtagc ctttttgtac gcttcggtgg gtcacggcgg cgccagcggt tacctggccc
    35041 tgatggccct ggccaatgcc gcgcctgcgg agatgaggcc gaccgccctg atcctgaatt
    35101 tatttgtttc tttgaccgca tttattcagt ttttccgggg cggccatttt aaatccggca
    35161 tcttctggcc atttatcctc gcttcggtac ccatggcctt ttggggcggg cgcatccatg
    35221 tggacgatga aatttataaa cagatcctcg gcgtcgcctt acttttcccg gtggcccgct
    35281 ttttgttttt tcagaacacc ccgcaggaga atttaaaaaa atcaaatact ccgctctccc
    35341 tgggtatcgg gggaatcatt ggcttctttt cagggctcat cggcatcggt ggcggcatcc
    35401 tgctttctcc catcctgctc ctgctcaaat ggacagacca gaaacaaacg gcggccatca
    35461 gcgccctgtt tatttttatc aattcagcat ccggacttgc agggcagctg accaaaggca
    35521 tcagcatcag ttcgcaaatg tatatcctgg taatcgtagc ttttacgggg gggctgaccg
    35581 gggcttattt tggggcgagc cggtttgatc aaaaggtatt aaaaacaatc ctggcggtgg
    35641 ttttgctctt ggcggcggtg aagctgttgt ttaaagtagg gtgagcagta ttccttatcc
    35701 ggggctaaag cccgatcttc tctatttcca tgaagacccc aggctgaagc ctggggttgg
    35761 ttaactcacc tcagtgaagt acaatttgac caaccccggc ctaaaggctg ggattgggcc
    35821 acccattttg atatgggctt tagccctaac tctaacgatt ctggaggggt tgcctttcga
    35881 ccctataatt gcattcgtgc tttatgctcc tttgactttt aacccttgac ctttcgacct
    35941 ccgttttttt tgctccctta aactttgacc tcccgctccc gaagtttcgg gatgcgggac
    36001 gccgttcacc ttcaatctat ttatctttct accaataaaa ctttcagatg tttaatccca
    36061 tttcatcttt atcggttctg gtcctccttc cattctatct actttcagcc tcccccatcc
    36121 gggtggaccg gatgacctgc gaatacctgg aaaatcccct gggcatcgaa atcctcaaac
    36181 cgcgctttgc ctggaccttg accagcccgg taaaaaacca ataccagtcc gcctatgaaa
    36241 tcctggtcag tgataatgcc gggtccctgg atctgggacg gggcaatcag tggtcgagtg
    36301 gcaagattct cgacagcaat acgattcaga ttgaatacgg cggcattccc ttaaaatcct
    36361 ttactcgtta ttactggaaa gtcagggtct gggatcaaag cggcgaagcc tccggatgga
    36421 gcaaggtagc ctggttcgaa acggctatgc tgagcccggc cgactggaaa gcttcctgga
    36481 tccatgacgg ccgcaccccg ccggataagg atgaagaata ctacgaagac gatcccatgc
    36541 ctcttttcag aaaaaaattc aatacggtcg gcgccattgc ttccgcccgg ctctacctga
    36601 gcggcctggg ttacagcgaa gccgaactga acggaaagaa aatcggcgac caggtcctgg
    36661 atcccggatt taccacctac cgcaagcagg cctattatgt ggtccacgat attacccgac
    36721 tgatcaaaga aggaaacaat acccttgggg tccgccttgg caacggctgg tggaatcccc
    36781 tgcccctccg gatgtttgga cgctgggacc tgcgcgcttt ccagcaaagc ggcaggccct
    36841 gcctgcgggc tgaaattcat ctcacctatc aaaatggacg gaaagaaatc attcccagtg
    36901 atcccagttg ggagaccgcg ccgggcccgg tcctgttcaa caacgtctac ctgggggaga
    36961 aatacgatgc ccggctcgaa aagtcctttg acgaccaggc cgcctggaaa aaggccgtgc
    37021 cggcgccagg cccggacggc accctgaccg cgcagttgct gccccccatc cggatcacca
    37081 aagaaatacg cccggtgcaa atccggcccg ccggcaagga tacctttatc gtggacatgg
    37141 gccagaattt tgcaggagtc gccaggatca gggtacaggg tcctgcagga acgaaaatca
    37201 gcatgcgttt tggggaagac accatgaaag atggccgcct gaactggtgg accacggtgg
    37261 ccgggcaaat caaagaggtc tggggtatct ccggggggcc cggagcgcct aaaaccgcct
    37321 ggcaaaaaga tgaatacatc ctcaagggag agggggtgga gacctggact ccccaattca
    37381 cctttcatgg gtttcgttat gtggaaatca ccggatggcc cggccagcca ggattggacg
    37441 atatccttgg cctgcgcatg aacaccgacc tggcagccca tggccgcttt gaatgttcca
    37501 atctcatgtt caaccggttg catgaagtca ttcaatggac ttttttgagt aatgtattca
    37561 gtgtacaatc cgattgtccc gcccgggaaa aaatgggtta tggtgctgat atggtcgtca
    37621 gcgcggaagc cttcctgtac aattatgata tggcgcattt ttattccaag actgtccgtg
    37681 attttgccaa tgaacagcgg cccgaaggag gcatcaccga aatcgcgccc ttcacgggaa
    37741 tcgcggaccg gggttacggc gacgggtccg gtcccctggg ctggcaactg gcctttcctt
    37801 ttcttcaaaa aaaactatat gaattttacg gcgacaaacg aatcctggaa gaaaactatg
    37861 ccgtctttga aaggcaaatg aattttctga agaccaaggc ggtatccaat ctttttcact
    37921 gggatatcag cgaccacgaa gccctggatc cgaaaccgga agccttctcc gccgccgctt
    37981 tctattacca tcatgctatc ctgatggctg agttttccgg catcctgggt aaaaaagaaa
    38041 acgaagccaa atacctcaaa ctggcttcaa ccatcaaaaa tctcatcgta tcaaaatacc
    38101 atgtccccgg taccggccgc ttcgacaatg cgacccagtc cgctcaattg ttcgcactct
    38161 ggtaccggtt tgccccggat cgagctgctg cctttcaaac ccttgtccag gaattcaaac
    38221 ggcataatat gcatctttcg accggtattt tctccaccaa gtttctgttt gatgtgctga
    38281 gggaggaagg gcaggccgaa ctggtttatc tcctcgccaa tcaacgcgat tatcccggct
    38341 ggggatatat gttggatcag ggcgcgacca ccttatggga gacctgggcc tatccggacc
    38401 gcgccccttc tcaaaatcat cccatgttcg gctccatcga tgaatggttt taccgttcca
    38461 tcctgggcat aaatccttca gcgccgggct ttgcgaaggt catcctcaaa ccccagccgg
    38521 caggggacct gagctgggcc cggggcgaat atcaatccat acggggcgtc ataaaaagcg
    38581 aatggaggac ggacggaaac aagtatttcc tcaaagcctt catcccggcc aatgtcaggg
    38641 ccgaaatatg gatccgttgt aaaccaaacg gcaggataat ggaaaacggg aaagaggtcc
    38701 ccggggtgaa atttacccag gggtatgcgg tggtggagac cgggtcggga gaatatttgt
    38761 ttgaaactga gctttgaaga agcgaacagc gagaagcggg cagcgagtta acttaccgac
    38821 ttgctgactg agcactgagg gctccatttt aaaactgcaa agaccgccat gaacgcaaag
    38881 gtcccgcaga ggaatttgac ctcgaacccg tagccaagcg ctcttccgct aatcacatac
    38941 ccttcaaggg gctcgccgca cattacttta tgtgaaaaac gagctaactt gctaactggc
    39001 caactggatc tcctccatca tcctcacacc cctggcccct gaaaggggac tgccgcacca
    39061 tactctagtg agagaagaga aacgagcagt gatcagcgaa ataactcact cacttgccaa
    39121 cttacaaact tgctaactta ccaacttgcc aactaactat ttcccttaat tttgcaccac
    39181 atgcacccgg gcaaaataaa ggttttattc ttcggccagc ttgccgagat gaccggcaaa
    39241 tccggtatgg acctgcagga tgtcacggat acccaggccg ccatagaaac actcaccagg
    39301 ctataccccc gcctgagcca aactccattc ttactcgccc tggacaagga aatcgtcgaa
    39361 ggcaaaatcc cattcaccgg tgaccatacc ttggccctcc tgcctcccta tgccggcgga
    39421 taaatcctga aaaatgagca gtcaccggat aaggaatatt tttgttcagg gtcccatcag
    39481 ccccgctttc attgcggaaa aaatcgccgc ccactcggat aaaacaaata tcgggggcca
    39541 cagcattttc ctcggacagg tcagggcgga tgagaaggaa ggtaaaaccg tggtgtccat
    39601 tgattacacg gcctatgagg aaatggccct ggagaagatg cacgcgatcc gccaggaaac
    39661 ttttaataaa tatgatttga cctgcatgca tgtctatcac agcctggggc aggtgaaggc
    39721 gggtgaaatc tccctatttg tattcacttc ctcggttcac cggaaacccg ccatcgacgc
    39781 ctgcgccgaa atcgtggaac ggatcaaggc cgaactgccg gtatggggca gggagatctt
    39841 gtcggatgag agtcatgtgt ggaaagagaa caggtgagtg gtggatggtg ggtggtggat
    39901 ggcaggtacc cttaccagct ttcctgatta aaccattata gaaaatatat aaaacatacc
    39961 gaatctttta ttccaaataa accataccat gtcctggaaa acagtcattc cccaccatcc
    40021 accatccacc aatcaccaac caccatccac catccacccc tcatggtaga catcacccac
    40081 aaatccacca gtctccgcaa ggccatagcc ggagccatcg tctccgtttc aaaacctgaa
    40141 acaatagaag cgatccgcaa caaaaccgta cccaagggcg atatcttcga attttcccgg
    40201 gcggccggct tgctggccat taaaaaaacc agcgacctga tccccgactg ccatccgctg
    40261 cccattgaat ttgcctccat ctcctatgac ctgaaagacc tggaagtgca tatcacggtg
    40321 gaagtgcata ccctttataa aaccggtgta gaggtggaag ccatgcacgg agcttcgctc
    40381 accgcgctta cgatgtatga tatgctcaaa cccattgaca aaggggtgga aatcaaaaac
    40441 atccggctga tctctaaaaa aggaggcaaa tccgatttcc ggaaagaaag ttacgatcac
    40501 ctccaggccg ccatcatcgt ctgttccgac agcgtatcca aaggcaaagc ggaggatact
    40561 tcaggaaagc tggccgctga aaagctcaaa accttcggct tgcaaatcaa aacacaaacc
    40621 gtaatcccgg atgatttcga caccatccag gaccgcgccc gcatgtacag ccgggatcat
    40681 gacctggtgc tttttatcgg aggtacgggc ttgtccccct atgaccttac gccggatgcc
    40741 ttaaaacccc tgctggataa ggaaataccc ggtatcgccg aaacggcaag gcaatatggg
    40801 cagcaaagga tgccttatgc catgctgtcc aggggcatcg caggatttat ggacaaggcc
    40861 ctggtgatca ccacgccggg atctccatcg ggtacccgcg agaccattga tgctttgttc
    40921 cctgccgttt tgcatgtttt ccggatccgg gaaggaaaaa gacacgatta acttaccaac
    40981 tcccccttta attcttaatt ctttaattct ttaattcctt aattcttaat ttctcctcaa
    41041 tgacctaccc caataccatt tcttcaacca ttcccgaatt catcgaagcc tccgtacaat
    41101 ccctgaggca gattcatgcc gaccagtggc caacaaggtc cgggccggag cgttggtcca
    41161 aaaaagaaat cctgggccac ctgattgaca gcgccatgac caatatccgg cgactcgtcg
    41221 tcacccagta tgaagccaat gaccgcatca cctaccgcca gaatgaatgg gtcgcttatt
    41281 cggcttacca ggatgctccg gtggaagacc tgatcacact ctggcggctc atcaacctgc
    41341 attatcaccg ggtggcctca aacgtcccca aatcatccct cagcctgacc tgcaatacaa
    41401 gcaatggtga acccctgttg cgtacgctcg attttttaat cacagattat tggggacatc
    41461 agcaacatca tttgagacag atatttgagg gatgaggatt gagggatgag gattgaggat
    41521 tgaggggtga gggcacctgc cgcacattac actccaactt gccaacttgc caacttacta
    41581 actgactaac tgactaactg actaactgcc tgcctcctca cttccggcgc caccaccgta
    41641 ccaaacaatt cgatcgacct catgaccttt ttatgttcga tggtgccgat gctcatctgg
    41701 gccataaagc gggtattgcc gaacagttcg tgttcgtaca aaatcttgtc aatgacttgc
    41761 tgcggactgc ctaccaggag ggcaccccgg ggtgaacgca tggcttcata ctgaccgcgg
    41821 gtggtaggtc cccagccccg ttcccggcca atacggctca tcacttcggc ataagaagga
    41881 aaaaattcat cggcggcctg ctgggaggtt tccgctacat aggtatggga gttgatgccc
    41941 agttggaatt tttgcgacgg atggccgtgc tgatcccagg ctttccggta caaatcaatg
    42001 aaaggtacaa attggaccgg ctgaccgcca ataatggcca gggtgagagg taagcccagg
    42061 gtgccggccc ggatggcaga ctggggcgtg cctcccgcgg cgatccaaac cggaagtttt
    42121 tcaacaaagg gtctcggata gattcccagg tttggaatgt cggggcggtg cttgcctttc
    42181 cagctgactt ttacctgttt attgatttcc agaagcaact gaagtttttc ttcaaacaat
    42241 tcatcgtagt cctccaggtg gtagccgaaa agcggaaagg attcaatgaa ggagcccctg
    42301 cccaccatca gttccgccct tcccgaagac aataaatcca cggtggcaaa ctgctgaaac
    42361 acccgcacgg gatcatcgct gctcaggatg gaaacggcgc tggaaagccg gatccctttg
    42421 gtttttacag ccgcggcggc caggaccaca gccggggcgg aaaccaggta gtccggacgg
    42481 tgatgttctc cgacggcata cacttccagg ccaagctgat cggccagttc gatttcttcc
    42541 atcagttcct gcatcctgcg gtggccgttt tgagaccatc cttgctctac aggggacaag
    42601 tctccaaaac tggatattcc taattccata aggtgaaact ataataattt aatttaaaga
    42661 cggattagac aggctggcca ttgcaataga cgatcgggac cgggaatccg gggaaaattg
    42721 tttttctttg ttcaaattaa gctctccaca tgtccattaa aaccggcctg tcctttctgt
    42781 tccttataat ctacaacagt ctatcggggc aatcggtttg ttattttaat gcaaaagtcg
    42841 tggatgatca gaccggggac ccgctggccg gcgtggtcat gagaatggat ggcctcgata
    42901 cctacaggac ttccgattcc ctgggctcac tgcggatggg agtaaaatgc ggggaacaca
    42961 cggtcaattt gcaggcttat ggatacaaac ctttttccta ccgatataat gtgcaggggg
    43021 acaaaccggt tgaaatccgc atggaaagcc tgctcaccca gttggaagaa atcgtcatca
    43081 gctcacagag cgaatccagg aacatagaaa gccccgccct cggagtaagc atgatgaacc
    43141 ttaaagcggt tcaaaaactc ccgccggcgg caggggaact cgatgtgctg cgcagccttc
    43201 aaagcctgcc gggcgtatcg gccgtaggcg aaggagccaa cgggatcaat atccggggcg
    43261 gttccgtgga ccaaaacctc attttcctgg aacacatgcc cgtattcaat ccgacccatt
    43321 tgttggggct gttctccctt tttccgacgg acggcatccg cgaactgcag ttgtacaagg
    43381 gttccattcc cgcccgttat ggaggcagga cggccggggt gctcgatgtg cgcctggccg
    43441 aacccgatat ggaaaaattc aatatgaaag gcggtatcgg aatgatttcc aaccgccttc
    43501 atgccgaggt gcccttgatc aaagaaaaaa tgggctggat gagctccaac cgattttcgt
    43561 tcaacgatta tctcatcaag ttgtacaatg ccgcgctaac ccccttcggt aataaaagga
    43621 tccccaataa caaacctcag tttatggacc tggccaataa aatcagctgg cgccctaccg
    43681 gccgggataa tatttccctc gtgagttata tcagttacga tgcctacctg atcgattccc
    43741 tgtacagcat cgcaggcatt gtgcccaaac aggcgaatat gaaatacggc cacaataatt
    43801 tttcggcccg gtggaaccat tatttttctc ccaaattcaa ttttaatctg cttgctgtgc
    43861 aatccaggta taatacctct acctcctcgt cagagattgg caccggattt gattttaaaa
    43921 ccggcctggt ttatcagaat atcaaggcgg agctgactta tgccccgact gccctgcagc
    43981 gtatcaacgc ggggttttcg gtcacccgcc tccgtctgaa accaggaagc ctgaagccgg
    44041 tggaaggttc caccctgttg cctgcggaac tgaaccggga attggggatg gaagcggcct
    44101 tttttatttc cgatgaatac gaattcaaca aaagactcct ggtagaattt ggccttcgag
    44161 cagtaaggtt ttggaacctg ggaccggtgg accttccctt gtttgaggaa gggagtccaa
    44221 aatccccttc caccattacg ggccggctga acctcaacgg ggcggaatcg gcttacaacc
    44281 gcctggaacc ccgcctggcc atgaggtata aattaaacga cctcacttcc gtcaaaatcg
    44341 gctacaaccg gatgaaccag ttcctgcaca tggtctcgaa caattccacg cccctgccca
    44401 acgtacgctg gaaatcaacc aaccgctacc ttcccccgga acaaagcgac ctggtttcga
    44461 taggattatt taaagatacc cgccttcgca actgggagtg gtccctggaa gggtattacc
    44521 gctggcaggg atttattttc gattatgtca atggggcgga actcagcatc aaccccgacg
    44581 tggaaaccca aattttaccc ggaaaaggaa aggcctacgg cgccgaactt ttgatcaata
    44641 agaaaaaggg gatcatgacc gggtggatga gttataccta tgcacgcagt ttccagaaaa
    44701 ttaccggcga atatcctgaa atccagcgcc tcaatgaagg ccgctggttc ccttccatca
    44761 ttgacaagcc gcataccctg aacatcctca ccaatttcca gacggaaaaa cacaacgcac
    44821 tgtccttgac ttttacctac agcaccggac ggccttatac ggccccggtc agtttttacc
    44881 gcaacgggtt taagtatgta cccgttttca ccgaccgcca caacgggagg atttcggatt
    44941 atcaccgact ggacttttca tggatcatca ccaatccttc catgaaacaa aaacgctggg
    45001 agggatcctg gatcgtcacc gtctacaacc tctacggaag gaaaaacgcc ttctcttatt
    45061 ttttcaatcc ggacctggcc cttttcaaac cctttaaagt gagcgttttc cccagtccca
    45121 ttttttctgt cacctacaat ttcaaatttg aataagatga ggctaacact gattctgggc
    45181 ataacccttt tccttacctg ccagtatccg gtggattttt ccgacttgcc ggaaacccaa
    45241 aaattcctgg tcatcgaggc ggacgttacg gaagaccacg tccgggtgga tgtccgttac
    45301 agcctcgaaa gagtgacttc cgcaggggct tataccctcc cccctccgcc ccaggctacc
    45361 gctttcgtcc ataccggcaa cggactgatc tatcaggtca gcaatacccg gggagtcctg
    45421 gatacttttt tcaagggccg ggtgggtcaa tcctacaaac tggtcgttca caccgaaggg
    45481 aaaacttatg aatccacgga agaaacgatg cggccctgcc ccgaactgga taccctggtg
    45541 gtgcaataca gccgggaaag tttccgggac aaagcggatc tcctgtacga cggttacgat
    45601 gtctatgccc gggccagcga tattgccggg gtagaaaact tttatcaatg gcactggata
    45661 cattattcca aagcgttcca gtgcaacaaa atcttcgacc gggcccaaaa cacggaagcc
    45721 tatgtcccct gttttcccca caattgctgg aacatcgtgc aaaatcaacg ggtgatcgtg
    45781 caaaaagaca atctccggga tggccaggcg ctcacccaat ccgtagtaag ggttccttat
    45841 gctaccccgc ccaaccggta ttacctgcgg gtggaacaaa ggtccatcac gccgaccgtc
    45901 tttgatcacc tcaagtcgct ggaaacccag accgaaacgg tgggcaccct ttttgacgtc
    45961 cctgcccaaa cccggttcag tcccaatatt gtgaataccg ccgaccggtc ggaaaagatc
    46021 ctcggcgtgt tcaatgtata ttcttaccgg tataaaatcg tggtcataga catgaaaaag
    46081 gaaattgcgg gcgctgtagt taaaacagtg attgaccgca cgccctttca ttccaacgcc
    46141 ttactctccg cggcttgcac ggaagagact taccgcactc agaagcggcc ggagggatgg
    46201 gtcgattgaa gaacagcttt actccgaggt ccgcagagca gaatcaggga ttttaaagag
    46261 gccttcagat attccagcct tccaattccc attttctgat cagggtgacc tctccttcca
    46321 ccgcaggctt tgttttgaca aaaggggcgg caggagcgcc cggtgcattg ctgcccccgg
    46381 cagcggcggc ttcctggctt ttggtcatgg atgccaggat cgttttgcat agaaactcaa
    46441 aggcgtccat tttaaaggcg ttgctttttt cggtttcata accgaaaagt ttggaaataa
    46501 aatggaagac ccggtccagc agcccgtgct tcctcatggg caatttacct cctgattcgt
    46561 cgtggtgatt gtccttggcc gagatgagat gcaggataat gtccgattta aatttatccc
    46621 agaaggtggg ttccgggatg cccaggctgg taaaaatttt ctggttctca atgaaaaaga
    46681 cctctacgtc ctgcagcctt tcccggtcaa taaatttacc ggtatgttgg gcgatgacgt
    46741 ccatcagggc gtcgttcagt gttttcccat attggttgac cgtattaatc tgccggttat
    46801 agatttgatt ctgcaggtac caaccttcct gaagcgtatt gggcatgagt tcctcttcaa
    46861 tacccagcat gagtcctacc acccgccagt ggaaaaaatg tacttcttct tccctggggc
    46921 tcagtttgac gcccattttc ttcagtcctt ccaggacagc cagtgaaaac agctggcagg
    46981 tgaggacttt gtccacctgg ttgatgggaa cgccattggg gaccccttct ttcaccgaat
    47041 ttttatccca gggctgttca agatccttaa ggatcatgtg ccgcataccc gcgtgcatga
    47101 gcctgacttt ctggatggcg cggatcccgc tgccttcggg cttataccat ccggggttgg
    47161 atacgtcaaa aataaactgg gcggtctcga gcacacggcg ggtagcctgt tccaccagca
    47221 gtttggtttc atgaaggacc ttcgagggct gttcacacat ataaccggtc ggcagggatt
    47281 taaaaaacaa cattcccagg atccggaaac cgtgattttc aaaaaaggcg acggattcat
    47341 caagcagcaa ttcttcttcc ggtgtgcaca aaagggtccc cgttttttca aaatattctc
    47401 tcagcctggg gtccaggtcg ggggagatcg gggaatcatt ccgtgccaga tccttcagga
    47461 agcggaaaat gttgggctgg ttttcgtcct gaaacagggc ttcggctgcc gcatcggcga
    47521 cggggtctcc gtgaaatcgc atcttatcca ggtactcatt ggtccataag gcggttcggt
    47581 ccatattatt cagcattagg atttacaaaa tacatttgga tctcctcatt gtgtttaacc
    47641 gtgattttgc cccggggttg gcattcaaac tgatccttca ccttgtggta ggtgatatcc
    47701 gaaatgttga tccgtcccgg ctcgccgtaa gactccagcc tggccgcaat attgacggta
    47761 tccccccata aatcgtaggc aaacttgtcg ctccccacga tgcccgccac caccggaccg
    47821 gtgtgaatgc cgacccggac ctggcaatat ggcaatccct tttccaaccg gtccctgttg
    47881 taccgttcaa tgaaacgcag gatgtccagg gcggcgcgga ccgcatggtg ggcatgctgg
    47941 ggatcgggat aaggcaggcc ggatacactc atgtaagcgt ctccaatggt ttttattttt
    48001 tccagcccgt ggtctttcat gatctgatca aaagcccgga aacaatcatc gaggaaagag
    48061 accagttccg tggccgacat ggctttggtc attttggaaa atccttcgaa atcagtaaac
    48121 aaaatggtga cttcggagta ttgcctgggc gccgatttgc ccgaggtttt caattcatgg
    48181 gcgatttctt ccggcatcat gttgagcagg agccggtcgg accttccctt ttcctcctcc
    48241 agcagttttt tctggttttc aatggttgcg gtgcgttcgt ttacccgggc ctccagggtt
    48301 tcatacaatt cggcattctg cagggaaatg gccatctggc cggaaaggag ttggagaatt
    48361 tccagccgtt cctcggtaaa gacatcggtg gccaggttgt tttccaggta aaggatggcg
    48421 acgatttcgt tgttgcgcat cacaggcatg cacaggaccg acaagggttt ttccttttcg
    48481 atataactgt cccgggcaaa acgaagatca aaggaggcgt tgttcaggat caggctttcc
    48541 ctggaattga acacataatt caccacctgt cttgaaagtc ccggcgactc ggacaatttt
    48601 gaggagacca ccacttgctg gcctgtccct tctttggaaa taaaagcttc cacactcagg
    48661 ccctctctgc ccggggtgat aaaaaagcct ttttgggcgc cggcattctg cacggcgata
    48721 tccaaaagac tgatcagcaa cttttcatag acgacctcct gggaaaggct gttggatgcg
    48781 cgaacaacgc tgttgaggtc gatggaagaa gtcccggccc gttccaccgt ttcgcgtccg
    48841 cctgtatttt tctccaggag gaaggggtgc tttttttcga gagccttgag cttgctgtaa
    48901 gctccccagt tggtgtagtc gatccaggct tcacggatca ggaattcagc gtgttgatta
    48961 atgcccaatt ggtattccag ttcggaccat cgttcatgga ccatgcccgc gatctgatgc
    49021 ataccccggg accgggcttt gcgctcagcg gaagaataat aacttacggc tttccggtaa
    49081 tccgcttctg cggcggcttg ttcagcgagg atcaggtcat atttggcgcc gaagttttgg
    49141 ggagaactca gagcccattt ccggatcatc tttaccgact tcttaagttt cttcgccaat
    49201 cgggcgtctg gtttttgttt caacagggcc gcgatcgtca gggcctggaa tataaagagg
    49261 tcgggatgtt cggccagccc gagaaaggcc ggatacatgg gttcgctcag gttgaaatag
    49321 tccagcgccc tgctgaagtc cccctgaaca taatggaaat accccttctg gaaataataa
    49381 atggccaggg gcatgggtat cttatgggtt tttgcccaat cctcaataaa tgcgaaatgt
    49441 tccttttcgc cggtgtattc atgaaaaatt tccgcggaag gttcccggtc aagaaactgg
    49501 gtgacgatcc gtgcataact caggaaaaaa tgcaatacca gggtgttgtg cgtctttcgg
    49561 ctgaaatggg cataagtcag cagtttttca tataccgatt ccaggtgttc cccattgtaa
    49621 taagggatgc gccacatgaa tgaaaccgca taccccgcgt agatcagatc attggtcgcc
    49681 accccggact ggtaggcgat ctggaggccc ggcatagccg cctcataagg ttgtttccaa
    49741 tgggacacac aaccttcggc gatgacatag actttagagc gcagggaatt gtcctggaat
    49801 ttttcattca accggattcc cagccttgaa aactcaaatc cgttttccgc atccttgagt
    49861 tgggcggtca cgacaaagcc ataaaaggca tatgccaggg cggattccac ggcgttccca
    49921 tgggtgatgg aataattgac catcttgagg ataaaataaa ccagcaggtt ctgcttttgc
    49981 agcaggtagg ctggccccca gatattcatc agaatgcgca tggccagttt gagcgcgggg
    50041 tcagacatgg ccggacggtg aaacagggat tccggggtct tccccctcag cttgaacttg
    50101 acccggacca gttccagcat gacctggccc tggcttgggt tgacgggcaa ggggataccg
    50161 agctgggcaa ggcctttttt tgccatgtcg atggcatggg catggcgctg ggtattttca
    50221 tacaggcgca ttttttgggc atagagctcc gctttctcca gggcgctgcc ggcgcggttt
    50281 atggctttgt caaagacctg ttcggactgg tcaaaattgc cgcagaggta ttcggcttcc
    50341 gcctgggaaa agaggaggtc ctggtagagg gcagattttt catcctggac caggttgatc
    50401 gccgcccgga tataattgag ggccggaata taggcattgg aattttttgc ccgttcaccg
    50461 gcttcgtagt tgagggtggc cacataattg atttcctccg ggttctggat gagatgggaa
    50521 ccgttgttga aatggttgac cacgtcaaac agcatatccc ggtcaggatt atttttatag
    50581 ctttcattga gcagcctcgc aacgcagagg tgggtcatcg cacgctgttc agggctgatc
    50641 agggaatacg ccgcctgctg gatgcggtcg tgattaaatt ggaaaaagac ctgcctgttg
    50701 tcctgcagac cggtcactcc gatctcttcc cagaggtcgt ccttgtaatg tttgttccag
    50761 ttgcccaggg gattgacaaa accggcctcc accgattccc aaagggcggc cgacacctgg
    50821 ttgacatggt attccgaagc aatgctgagg ttgtgcaggt caaaacgatt gccgagaaaa
    50881 gcggcaatgt tcaggataaa ctgccccccc ttgctcagct tttttatctt gagttctacc
    50941 aggtcgatca cattggcggt gacggccaat ttgtcgattt ccgcttcgtt ccatttccat
    51001 tccaggtcgg catccgaata atacagcagt ttttcctctt ccagtttttc caggaattgc
    51061 cggataaaaa aaggattgcc ctgggatttc ttgatgagca gggcggacag cctggcggtg
    51121 gcttccatgc tggtatgcag ggtatcggcc aacagttcat tcacggagag gccgtccagt
    51181 tccttcagct ggatttcatt ggtccggtcg ggcctttgtt cgcgtatcct gcccagcatc
    51241 atcatgaggg gatgggagag attcacctcg ttgtcccggt aggccccgat aatcataaaa
    51301 tattttatgg ccggactgag cagcaggtat tcgatcaggt cgagggagcc actgtcggcc
    51361 cattgcaggt catcgataaa aagcaccagg ggatgttcgg ccgtagccag gaccgagata
    51421 aatcgttcaa acagtttaag gaagcgggcc ctggattcgg tgaatccgag tttgagggca
    51481 gcttcttgct ggccgatgag caggttcaac tccgggatga tgtccgtgag cagggaaccg
    51541 ttgtcctcca aaataggctg tagttcgccg gcgatctttt gggcggcttc ctggccctgg
    51601 gcaaggacgt accggaccag gcttccgaag gcctgtccga atgcattgaa gggaaggtcc
    51661 ttttggtatt gatcaaattt accgaaaaca aaaaaaccgt tttgatggat gatgggtttc
    51721 tgcacttcat tgatcagcct tgttttgccg atcccggaat acccgctcac catcaggccc
    51781 atcgtatggc cttcccgggc ccggttgaag gattgcatca ggacatccag ttcccggttt
    51841 cgtccgtaga gcttttcaga gatcctcagt ttggagtaat ggtcgtgctc gcccagcacc
    51901 atcccggagg catggccttt actgaccctt tcctttatct ctttgatatc cgtcttgagg
    51961 ccggaaatgc tctggtacct gttttccgca ttcttggaca tcatccggag gatgaattcc
    52021 gaaacgatgg gaggcacggc ccggttgatc aggtggggct cgacggcttg tttggcaata
    52081 tgggcatgaa tgatttctga aaggttgccg tcaaagggca tccggcccgt gaccagcttg
    52141 tagaagagca caccgagcga atagatatcc gtgcgataat cgatgctccg gttcatgcgt
    52201 cctgtttgtt ccgggctcat ataaaacggc gagatcgaat cctgtacgga atggtcgatc
    52261 cgctgggccg tggtggatag cgtggtggca tggccgaaat aggacagctt gatgtttttc
    52321 agatcgggcg aaaccagaaa agaggacgga ctgatgtccc ggtggacaac cccccgggca
    52381 tggaccttgc cgacacaatc cgcaagcagg taagccaggt ccaggaagtc gggtatagac
    52441 aaatgcaact tgtcgatctc cgactccagc gtattgccct gaaacatttc tttaatcacc
    52501 ccgatgccgg cttcctgccg gatgatctca tgggctttga ggatctgcgg ggattccaga
    52561 atgctcgaaa tctcatagtc gtttttcaag ccgaccaggg attcaaagtc atacaggcgc
    52621 gaagcgggca attcgagcag gtagggctgt ccgtcacggc cggtagcctc gaagtaattg
    52681 cacctggtgg tttttatctt taatggcccg atcgaatatt catacataat tttggtgacg
    52741 cctccttggt taatggggtt ttacggcaga aaagaccagg taattccata ggtctttttt
    52801 cagggccttg tattggtaga aggtggacca taggtttttc tgaagggttt gatttctctg
    52861 gttcatgagc ctcatcaggt ttccgatgac ccatccgggg aaaaaataat aatacagcct
    52921 ctgggcggag ggcttgattt tatctgtaat gtttccgcag tttatttgtt tgaatccgca
    52981 ttcctccatc atcgcggaaa aacgtccgga aagttcaaaa aaaggaacgg cccaggcatc
    53041 ggcccagcgt tgcatcaggg gccttccctg cggttcatga cccgccggaa gggaaaaaaa
    53101 atcggcaaca gccagcctcc ccccgggttt gagcaggcgg aacgcttctt tcaggaaggc
    53161 tttcttgtcg ttggcatggc atacgctctc catagcccag acggtgtcaa aactcgcagg
    53221 ctcaa
//
LOCUS       DAOQXT010000022        50678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_749_length_50678_cov_6.222251, whole genome shotgun sequence.
ACCESSION   DAOQXT010000022 DAOQXT010000000
VERSION     DAOQXT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 50678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50678
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_749_length_50678_cov_6.222251"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PKM27_05610"
     CDS             <1..737
                     /locus_tag="PKM27_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR06775.1"
                     /translation="IQDLDRGVVMGQQTYGKGLVQNTREIGYNSQVKLTTAKYYIPSG
                     RCIQAVEYENGEPKNIPDSLRAEFKTRGGRKVLDGGGIRPDVVIDAGKQPEILKQMIE
                     KQLPFKYATHYNLAHPSIKPVREFSFTEYDDFKKFYRESGFDFTSSAESALEAFRAKA
                     STEESWSRIREDVQALQSKLIQDPGKLLDEHRDLISRHLEKEIIPRYYLQKGKIEKAL
                     QVDMEIQEAIRLLGDPTRYEALLKKK"
     gene            739..1431
                     /gene="tsaB"
                     /locus_tag="PKM27_05615"
     CDS             739..1431
                     /gene="tsaB"
                     /locus_tag="PKM27_05615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNR06776.1"
                     /translation="MTPDALLLHIETSTEVCSIAISKGPHLLSNEQERQGRKHAQWLP
                     LMIESARQKASVGLKDLDAVCYSRGPGSYTGLRVGLSTAKGLCFGLAKPLIGVPTLLA
                     MAHSGGEPGPFSLFVPMIDARRDEVYMAVYGPGWQVIAGPESLVLSPGSLDEWLEKGY
                     SVHLFGDGSVKAKTILKPHERLNFGPAEADARYMIKPGLDQFNLSRFENLAYAVPEYL
                     KVPNYKKTLINT"
     gene            1592..1930
                     /locus_tag="PKM27_05620"
     CDS             1592..1930
                     /locus_tag="PKM27_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR06777.1"
                     /translation="MKKQKQEIVTLKKGPKDIQLDYTDLRRAVLVLRAVNHKLRQRII
                     EVLEESDNMTVTDIYIKLRLEQSVASQHLAILRRAGVVITDRQGKFIYYSLDKERLNQ
                     ISKLIEELVG"
     gene            2146..2451
                     /locus_tag="PKM27_05625"
     CDS             2146..2451
                     /locus_tag="PKM27_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR06778.1"
                     /translation="MKKTKVTFDQEKLQYSSELLRALAHPLRLKILEFIDRHEVINVN
                     KIYNSLKIEQSITSQHLKILRGAGVVDNNKDGKFKHYQIDYQKVANAVKAINNFMGK"
     gene            complement(2570..3601)
                     /locus_tag="PKM27_05630"
     CDS             complement(2570..3601)
                     /locus_tag="PKM27_05630"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNR06779.1"
                     /translation="MNSGWLFFFNLNLNLNLNLNLNLNLNLNLNLNLNLNLNLNLNLS
                     QVLGTYPQPKSSPPIHISSYLCPSMEGTIRSASSIQRRSLITIFTVLIVLVLDQWLKI
                     WVKTNMEYGSEFRILGLDWARIHFVENEGMAFGISLGGNTGKLLLSCFRILAVGFLIY
                     IIYKLVRSRESTGLLVCFSLILAGAIGNILDSAFYGLIFTESYYQGGLAEYNPSKGYA
                     PFLFGRVVDMFYFPLIDTYWPEWMPLLGGKSFQFFRPVFNVADAAISSGVIALLLFFR
                     SFFKAPHEEMIHAKPEARAEDSLVVDGQEKQAEPSALIPEVASDDQNLPDLQATDQDQ
                     PEGDKTEEA"
     gene            complement(3635..5167)
                     /locus_tag="PKM27_05635"
     CDS             complement(3635..5167)
                     /locus_tag="PKM27_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNR06780.1"
                     /translation="MKRIMKNYIKLLFLTLSLVIVSCDDGFDELNINKNAATAINPAF
                     ILNNAVISASYSNVTIIYEIGIVQQMVSPNSGVLTGANFNQDNRDATNQNWVRGYRNV
                     IRNTTDVIRQTSEQPNRSNLLNMAKILQSFAFMVLTDSYGEVPYFDAGKGYSDQITLP
                     KYDAQQAIYADIIKTLTEATAALDASKPIETADIMYGGDVAKWKKFGYSLLLRAGMRL
                     TKVDPTLAQATVQKAVAGGLMTSNADNAYTRHDANYTNAIGQWMNSTEAANSYIGAPF
                     LDHLKRTKDPRLKAIAVRYVGAKSGPEQTAARANRDTAVQIGMPFGYDNNGIAAQAAA
                     RGLASFYDFSQVDRTRFGKNTAPMFFVTYSQTSLLLAEAIQRGWASGSAADSYKNGIK
                     AHMDQCAIHDANSAVPTEEADAYIAANPYDPAKGLEQINTQYWISSFMNGPEAFANFR
                     RSGYPALMPNPFPGKGIKGDFIRRLTYPNSEISVNSTNVQEAISRQGADDLDTRVWWD
                     KQ"
     gene            complement(5170..8328)
                     /locus_tag="PKM27_05640"
     CDS             complement(5170..8328)
                     /locus_tag="PKM27_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNR06781.1"
                     /translation="MNKLRLLFFVLLLSVAGILQAQTITGMVKAGEDGSPLTGVTILI
                     KGTNTGSVSDVDGRFSIEATPQSILVFSYVGYQTLEVAVGNQTNLEVTMESTVESLGE
                     IVVTSLGIRKEAKKLGYATSTVTKELVAENRSPNFINSLQGRVAGVNISSLGTGPGGT
                     SKIRIRGQSSIAGQNNPLIVINGVPIDNTNFGYNPGNAGADNSIGLRGGGVTSDGGDG
                     LSSINPDDIESMTILKGATAAALYGSRAKDGVIMITTKTRGDAKSKGIGVTFNSNYTN
                     DTPLDFTDYQYEYGQGENGVRPTTPNPTSGQWSFGEKFQPGMTQVLFDNVTVPYEPVF
                     DRIKKFFRNGQNFANTLTLSSSNEKGGFNLSFNNTENQGIISNNSLNRKILNLGFAYD
                     LSKKLSFSGSANYSKEKNNNPPNIANQDNSIPTSLYNLANSMPLELLDAKKYNAQGNE
                     FIYSRFMNRTNPYWTLAEQFNHVVRDRLYGNLTLKYNLTPWLSIQGRAGQDYWTRDQD
                     YNNFPTGHASLAAAPVGFVNGSFTQESRRFRETNMDLLISASREFGDFGIDLTAGGNK
                     MYRRADQNNVFVQDFVVRGLYTVANGRVKDPIYSLSERAVNSIYGSADVSYKRILFLN
                     GTLRNDWFSTLSPANRSILYPSVSGSFVFSDAFSGMPSWLSFGKLRLAYAEVGSDTDV
                     GPYANNLFYGVNANLFPNPSGALQPIGGASGNVLPNANLRPMRTSEKEAGLELKLFQN
                     RAALDFAVYQKITEDQIVSAQISDGSGFTNTLINSGTSRTNGFEGLLNLVPIKSGGFQ
                     WDITFNGSFNKTKVLKLLTDTEGERITVGTHVFNGELRQVVGQEMGQIYGFGYRRDAQ
                     GRKIFGGNGIALRTLDLISFGSALPKWTGGINNGFTFNDFSFSFLIDFKLGNKMLSGT
                     NFNLTRHGLHKQTLAGRDGIVGDGVNEKGEVNTVKANIQPYWEVVRSLALIEPVIYNG
                     GYWKLRQITASYDLTKFIPSSVPISGVKLSFVANNVLMLKKWVPNIDPESFGYTSDNL
                     VGMESTGLPTTRSIGFNLNVKF"
     gene            8535..9707
                     /locus_tag="PKM27_05645"
     CDS             8535..9707
                     /locus_tag="PKM27_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06782.1"
                     /translation="MQGLTVLFSVLWCSVTADAQVRMRTVYAGGMDPQSRDSHSVFMD
                     PLVTGNRLLLVHLPASFDKTGRSSAFCTLAAQSGYRVISLAYPGISNMYSSCEHSTDA
                     NCYENFHLELFDGNNRAPQIEIDSGENILHRLVNLLQVLELKYPEEEWNQFLEGPDQI
                     RYSSTIWTGHSDGAGHALAVAKYHQVHRVICFSGPKDFNLHYYLPPAWFHSGLWKTGK
                     SEIFAFAHTADEYAFQREIWDSLGLGKYGPPVNVETNPPPYLSSHQLISSQSLPAGHI
                     HGSTILDDQMPRKDGKSLYEPVWKYLLDIRSTPVANLNTASSIAVYPNPVHRGNSIFL
                     NADIKNPGLHLFDIQGRLLRIFRDGKMYIDQDLPAGPCFIQYNNGNRLQVIRLTIL"
     gene            complement(9709..10962)
                     /gene="odhB"
                     /locus_tag="PKM27_05650"
     CDS             complement(9709..10962)
                     /gene="odhB"
                     /locus_tag="PKM27_05650"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNR06783.1"
                     /translation="MSVIEIKVPNMGESITEVTLSKWLKKEGDLVKMDEPICEFESDK
                     ATLDLPAEAAGKLHILVPDGSDLKIGAVIAQIDTAHTSAAPSPVQAAEPAKTETPAPS
                     AVRENKSSEPIASPAAAKIIREQGIPSTEIAGTGKAGRITKADALQAVSREQETKPVP
                     SEAKAGQAPDKPVSLEPGKPSASFSRSERREKMSRMRRTISRRLVSARNETAMLTTFN
                     EVDMTEIMALRNKYQDAFVARYSIKLGFMSIFAKACSKVLLEMPEVNAFLDGEELVYH
                     DYTDISIAISTPNGLVVPPVKNVESLNFHEIEFKIKELADKARAGKLSLDELSGGTFT
                     ITNGGIFGSMMSTPILNEPQSAILGMHAILDRPVAVAGEIKIRPMMYLALSYDHRVID
                     GSSSVTFLVKVKKLLEDPVSLLLNL"
     gene            11069..12328
                     /locus_tag="PKM27_05655"
     CDS             11069..12328
                     /locus_tag="PKM27_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR06784.1"
                     /translation="MKNSVRIQLSVMMFLQFVVWGAWYGQLSKYLFAINFTGAQVGNI
                     YATFSIAMIISPFFIGMLADRFFAAQKVLGVLNILGAVLLYALTQIKEYNAFYFVMLL
                     YCLTFAPTIALTSSISMRQMKDPEKEFPPIRVLGTLAWIAVTNLVGFMGWGDKATIFY
                     VSMITALGIGLFSFTLPNTPPTISGPVSVGQVIGKDSFVLFKDRSFLIFFIASILICI
                     PLSFYYALGNPSITDYYKAAFMAANPGQALPLTFFVENKMSLGQASEVLFMLLLPLAY
                     SRFGIKNILIIALVAWIFRFVFFGYGNAGSNEWMLYAGILLHGICFDFFFVSGMIYTD
                     KKAGDKIKSQAQGLISLATYGVGMFIGSVLSGVVKDKYTTGAGADALTNWTGVWLVPA
                     GIAAVVLIFFFLFFKDRNPAPVKQLVS"
     gene            12565..13281
                     /locus_tag="PKM27_05660"
     CDS             12565..13281
                     /locus_tag="PKM27_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06785.1"
                     /translation="MKTRIFTSFLFSLFTVAIVAGDLDGYWYCADHHSNIEVMYTTKS
                     IKVRNPDREPDHWELYNRFENNRYRDNRGNVYYFNGSGLEWNAPGRGIAHHYTRNSSP
                     SGGNWRDPGRNDRGWGDRPGNAGGGWNERDRGGWTNDRDGWNGFYKPYEGKWHNHSTG
                     THIHVDLSRRSLRIKFHGEKWHEVFERNRGLFVDRKGNEFIFRGEGIEYRAHEGDLIM
                     KFYNDDRCFHRDDYRAEYWR"
     gene            complement(13375..13800)
                     /locus_tag="PKM27_05665"
     CDS             complement(13375..13800)
                     /locus_tag="PKM27_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06786.1"
                     /translation="MSQISTLSINLHTGDFDGAGTDGDVYVGLGGREFYLDTSNDDFE
                     RNASKVYQLGSGANILHAAYNDPNKPQINTEDLDLYPVYIRFNPKNRDDRWNLSYVDI
                     MVNGAIAYQSLGLDQQGGIWLGIRSGLFYYLKKHRDSRP"
     gene            complement(14035..14394)
                     /locus_tag="PKM27_05670"
     CDS             complement(14035..14394)
                     /locus_tag="PKM27_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR06787.1"
                     /translation="MPFIDFKSMAPLNIWEGINGPIAYSDQLLFGYFTIDDGAVLPTH
                     QHPHEQWSHLIEGEFEFNIGGEILHMKPGMSAFIPGNVPHSGKALTKALFLDCFYPVR
                     EDFVEKERQSYEGERLG"
     gene            complement(14500..17769)
                     /locus_tag="PKM27_05675"
     CDS             complement(14500..17769)
                     /locus_tag="PKM27_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNR06788.1"
                     /translation="MKIRIPLLLFLVLSTFSAWSQMDARLFRYPDVSDKQIAFVYGGD
                     IWLVPKEGGLAFKITSSPGEESFPRFSPDGKSLAYSANYDGNTDVYLMSLQGGGVDRL
                     TYNSFPDRMVDWHPDGRSLLIASSKESGTPAYRQFYSLPVIGGLPDKLSIPYGELGSY
                     SPDGSKIAYVTRITENYPFKRYRGGMASDVVVFDLKNQTALNITKNSATDGKPAWHKN
                     SIYYVSDAGTQKRRNIWVYHTDRQTRQQITQFTDYDINHMSIGPEELVFEAGGKLYLL
                     NLDNHRQREVQINVVADLASTMPRQVRVNNMIRNFESSPDGKRVMIEARGELFNIPSE
                     HGYVQNTTNASGSFERMPAWSPDGKYVAYWSDRSGENEIYLKAMDGKSADEPLSKFGG
                     GYGFRLFWSPDSKKIALIDYMQAIKIIDLATKEVTIADHTRNMVWGNLNGFRINWSAD
                     NKWITYYKATDNTNPAIFVYNLETKKVSQLTSGYYNDYEPVFDPKGKYLYFFTDRSFQ
                     PSYSSLDATWIYPNTTQIAALSLQSATSSPLAPKNDEIKITEEKKPGTDSSQVKKPEA
                     GKTRDSLIIDLENAESRLVILPVPAGNLGNLNAVDGKIIYHRRPNTGAGGNISPVQFY
                     DLEKREEKSIYANANRYVLAPTGSNLVVQDNDRIGIIKINPDQKIDKALRTAEMTMSL
                     VPKAEWTQLFNDTWRRYRDFFYDPGMQQVDWPAMKTRYGSLLQDAITRLDVTNILIEL
                     ISELSAGHTYAGGGDMEQVEFRPTGFLGIDWALDQGAYKIKRIVRPAAWDNEVRSPFD
                     RPGIDVKAGDYIFSVNGITIDRARDPYAAFEGLAGRVVSLRIGKTANAAEAKEVIVET
                     LNPQQEGRLRHLEWIESNRKAVDLASNGNLGYMYMPNTTTQGQTELLRQFYAQVDKKG
                     FVIDERFNAGGQLGDRFLEMLNRPTVYYIGWRNGYDLPIPTKANDGPKVMLINGWAGS
                     GGDAFPWGFQQLKMGPIVGERTLGILVGPATTHQLIDNGTITVPDARLFGTDGKWFAE
                     GHGIEPTVEVWDDPAALARGKDPQLLRAVEETMKLITTHPRKMQSRPPYEDRTAKGIK
                     D"
     gene            complement(17766..17924)
                     /locus_tag="PKM27_05680"
     CDS             complement(17766..17924)
                     /locus_tag="PKM27_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06789.1"
                     /translation="MEGTSSFRSNLNLNLNLNLNLNLNLNLNLNLNLNLNLNLNLNLY
                     ITTTQSST"
     gene            18018..18938
                     /locus_tag="PKM27_05685"
     CDS             18018..18938
                     /locus_tag="PKM27_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR06790.1"
                     /translation="MKPNCLILWFFFAGTCLAQQPLKPNNPYTGPGTAPVQPTVVQSE
                     EEAVITVIYRLFDAMKAADSAAARTLFTPGARLYTPVTKASGETTLQEETVAAFLKSI
                     GTKRPEAIDERILKYKIDIDGPLAQVWADYNLYVGTKFIHCGIDVFHLYKSNEGWKIF
                     ELADTRRKTGCQPDPLDEVAAFLDTWHAAAANADAEKYFGAMTPDGVFLGTDATERWT
                     REEFRIWAKPQFDSKKGWNFKATKRNAAFLENNTIAWFDEDLSTWMGPCRGSGVLVKT
                     SEGWKIKQYNLTILVPNEKVQEYLKVIGLR"
     gene            18994..19278
                     /locus_tag="PKM27_05690"
     CDS             18994..19278
                     /locus_tag="PKM27_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06791.1"
                     /translation="MVTLRIEHKIASMAGWRKAFDSDPLNRKQAGVKHYRIFQPKEDQ
                     NLVIIELDFDHLEQAEAMRKALNQLFPKIEGTLVFGVQVKILDLIESTDL"
     gene            complement(19340..20629)
                     /locus_tag="PKM27_05695"
     CDS             complement(19340..20629)
                     /locus_tag="PKM27_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNR06792.1"
                     /translation="MFNRRKFLTGLGAGAGLAALPFPSLAKWTDEDNTADTIIDAVEV
                     IRITGPYTSISGVNRQYQVQQIHIYPEWRPGLYSDRTDSRETTSNISQYYLQIRTRGG
                     LTGFYGAIDGECVTPVLQQLKPFLLGKNALAIESLWDGMYRNNRHGRAGHYMMALSAV
                     DNVLWDIRGKYYNTPVYALLGGPTRKEVLVYGSCLSFTVESGKAGPKARQLFNEGFKY
                     QKWFMAYGPGSGPQGYNHSVGLMEELRGSLGPEALIMVDAYMGWDYPFANAWCREVEK
                     YRPYFLEEAFPSDRLESFVQLSQHSSIPLATGEHFYSRWEALHFLKAGAIQVVQSDPE
                     WCGGVSELVKTCHIASAFGAKVIPHGHNIHAALHVVASQSPGVCPFGEYLINHMPYKL
                     NFQKNPLLTANGILPLPTLPGFGIELDESKVEKKEVV"
     gene            complement(20706..22811)
                     /gene="feoB"
                     /locus_tag="PKM27_05700"
     CDS             complement(20706..22811)
                     /gene="feoB"
                     /locus_tag="PKM27_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNR06793.1"
                     /translation="MKKIAILGNPNSGKSSVFNRLTGLAAKVGNFPGVTVEKKKGTVR
                     LPSGQWVDVIDFPGVYSLHPNSKDEFIVTNILTHPDDADFPDLVLYVADIMHLEKQLL
                     LFTQLIDLGLPVIMVLTMKDLADKDRFSIDLNVLKATWDIPIFAINARNQESMPAILQ
                     EMDRQLAKPSPLSQQQYKLSYQEQALVNELNDVVRTNNAYQSLLVAHYHQQLNYLKPG
                     QSEQIGQINTRHGFNSIASQIRETMQRYDFFTSVVRKSASAGTFKVSRFSEKADDVLT
                     HRWWGILIFVIVNFFIFQAMFTWASYPMDWIETVFGWAGAQVKNVVSSPGWSSFISDG
                     ILAGLGGIIVFIPQIAILFFLITVLEDLGYMARAVYLFDRLLLKFGLNGKSLVSLLAG
                     GACAIPAIMSARTISNQKERLITTLVTPFISCSARIPVYTILVGFVVSSSHRTGPFNT
                     QGVLFMGLYLLGIITALLAGWILKQIIKSEDRSFLMIELPDYKVPDFKVAFQTALSKA
                     WSFVVEAGKIILIISMVLWVLSSYGPQSKMKASEQEVGQIRIEKNLTEEETASLLANK
                     KLEASYAGTIGKWMEPMIRPLGFDWKIGIALFTSFAAREVFVATMSTLYSLGSTEDYS
                     TITERLAAEKDPETLQPRFTMPVAISLLLFYVFAMQCMSTMAVVRRETGGWKWPLIQF
                     FFMCGLAYLASLVAYQVLS"
     gene            complement(22808..23059)
                     /locus_tag="PKM27_05705"
     CDS             complement(22808..23059)
                     /locus_tag="PKM27_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNR06794.1"
                     /translation="MFKPILAKNLREIKYGKVVGFSDESISKLLISKGIIIGSLATLV
                     RESPFGQTYYVKIDGLRFGLRKEELDVIQVTETEAQISS"
     gene            23229..23623
                     /locus_tag="PKM27_05710"
                     /pseudo
     CDS             23229..23623
                     /locus_tag="PKM27_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
     gene            complement(23722..23847)
                     /locus_tag="PKM27_05715"
     CDS             complement(23722..23847)
                     /locus_tag="PKM27_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06795.1"
                     /translation="MKKRKWVVNGYPLNLTRDIRFWFWLICMLAFLLTTISRIVN"
     gene            complement(24031..24711)
                     /locus_tag="PKM27_05720"
     CDS             complement(24031..24711)
                     /locus_tag="PKM27_05720"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="catalyzes the formation of pyruvate and glyoxylate
                     from 4-hydroxy-2-oxoglutarate; or pyruvate and
                     D-glyceraldehyde 3-phosphate from
                     2-dehydro-3-deoxy-D-glyconate 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNR06796.1"
                     /translation="MFKRYPADRHEITADIYQTALLPLFYQADLEKAKQLVQVCYETG
                     LRVLEFTNRAAFAYEVFSPLSAYCKNNFPALRFGAGTITDTGTAAMYLQSGADFIVMP
                     SLQPEVISLCNKRKILCIPGCGSVTEISKAEELGCEFVKLFPGNHFGPGFVKDLLGPM
                     PWTSILVSGGVEPVKENIQSWIKAGAASLALGSKLFTPAVLRGEQLDDLGQSLADCLR
                     WAAEARLD"
     gene            24864..26597
                     /gene="dnaX"
                     /locus_tag="PKM27_05725"
     CDS             24864..26597
                     /gene="dnaX"
                     /locus_tag="PKM27_05725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNR06797.1"
                     /translation="MSDFLVSARKYRPQRFADVLGQEHVTRTLKNALLRNQMAHAFLF
                     TGPRGVGKTTCARILAKVINCENALKNQDAEPCNACESCRKFDEQLTFNIIEIDAASN
                     NSVEGMRNLIEQVRYLPQQGKYKVFIIDEVHMLSNAAFNAFLKTLEEPPAHAIFILAT
                     TERHKIIPTILSRCQVYDFRRIQPEEIVKQLEEVAKDQEIKTEAEALHIIAHKAEGSM
                     RDALSIFDRIASFGGKQVTYAGTISNLNVLDHEYFFNLVYGMLREDSMGILKTFRDIH
                     QLGFEGNHFMDGLADHLRNLLVSKDPGAAQLIEGSQDLKAKYHAQAQGAPVSFILDAL
                     AIVNECDLQYERARNKRLHVELALIRMCYLQRLSDAIPSFPQEAEKKKPEPGPAPPQV
                     IISPPPPVHLAVFDQDPVEFQRPAAPAEQKLPNHYDTPKIRSLKDIQKKAAQSIQANP
                     DSAVELKIETFEQAWKEFVDTVEVLSLKTVLSQVELALEQTKLLIKVGSAHARNIIQL
                     ESSLLEKLRNSVSDKRLEIAIVIDPEKAKLMESQVKKPVSLKEKYELLVQANPKVEDL
                     ILALKLRIDHG"
     gene            26622..27668
                     /locus_tag="PKM27_05730"
     CDS             26622..27668
                     /locus_tag="PKM27_05730"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNR06798.1"
                     /translation="MWKVLIRPVLFLLDAETAHHFTLGLFKWICRIPGCRRLIKVLFA
                     LPEQQAVEVFGLKFKNPVGLAAGLDKDGKYLHELYLLGFSHIEIGTVTPRPQTGNPRP
                     RLFRLPKDQALINRMGFNNLGADAMAQRLRSRNKPPDLILGINIGKNKDTPLEQATAD
                     YLACFEKLHPYADYFTVNISSPNTPGLRGLQDKAPLNELLGAIMKANKNLAQPKPVLV
                     KLAPDLDDAQLAEAKEIILSHGIDGIIMGNTTLDRSGLRTEPERIEKIGAGGLSGLPL
                     LKKSNERLSALTGLPGKIPVIGVGGIMNGADGRSKLREGAVLVQVYSGFIYEGPGLVR
                     QICAALNRQTDAPG"
     gene            27732..28172
                     /locus_tag="PKM27_05735"
     CDS             27732..28172
                     /locus_tag="PKM27_05735"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNR06799.1"
                     /translation="MNKKGYRSNAGEGGAIPKGGNGHYSIALIAHDGKKLDMASFIFR
                     HHAMLKNMILYATHTTGTYIEKEGIPVIKLLSGPRGGDAQIAAMVAEGKLHAVIFFRD
                     PLDAHPHEPDVQMLMRVCDVHNVPLATNPATATMIIDTLAVKQP"
     gene            28902..31178
                     /locus_tag="PKM27_05740"
     CDS             28902..31178
                     /locus_tag="PKM27_05740"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNR06800.1"
                     /translation="MSTPFDLEMIRAVYASFGDKVKNIRQQLGRPLTLTEKILFTHLH
                     EDSPLKDYERGEDYVFFAPDRVAMQDATAQMALLQFMQCGRDKTAVPTTVHCDHLIQA
                     KSGADTDLKTALDKNSEVFAFLSSISDRYGIGFWKPGAGIIHQIVLENYAFPGGMMIG
                     TDSHTPNAGGLGMIAIGVGGADAVDVMSGMPWELKMPKLIGVKLTGKLSGWASPKDVI
                     LKVAGILTVEGGTGAVVEYFGPGAQSMSCTGKGTICNMGAEIGATTSTFGYDASMGRY
                     LRATGRAVIADLADGIAQELTGDEACYKNPEEYFDRVIEIDLSKLEPHVNGPYTPDLA
                     WPLSEFAAAVKEKGYPAKLEVGLIGSCTNSSYEDLDRAAFVAKQAKEKKLKVKSEFTI
                     TPGSEQIRYTVERDGQLQAFEDIGGIVLANACGPCIGQWARHTDDPNRKNSILTSFNR
                     NFSKRNDGNANTHAFVASPEIVTALAIAGTLTFNPLTDTLINEDGQSVKLDPPQGEEL
                     PSKGFDVEDAGYQAPSADGSSVRVEVREDSDRLQLLTPFKPITQEQVKGMRILIKAKG
                     KCTTDHISMAGPWLKYRGHLENISNNCLIGAINDFNGEANKVLNLVTNEYMAVPDSAR
                     TYQKAGVGTVVFGEFNYGEGSSREHAAMEPRYLGVKAVIVKSFARIHQTNLKKQGMLA
                     LTFTNPDDYDLIRQDDTVDIGGFEDFAPDEALYVVLHHADGTMDRFEVDHNYNEQQIE
                     WVKEGSALNKIRKDLGVS"
     gene            31456..33141
                     /locus_tag="PKM27_05745"
     CDS             31456..33141
                     /locus_tag="PKM27_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNR06801.1"
                     /translation="METSYDAIVIGSGISGGWAAKELCEKGLKTLVLERGRDIKHIKD
                     YPTATMAPWDFQYRGRVSREYIEKNPLISKAAGFGDDTKHFFIQDADHPYIQEKPFDW
                     IRGYQVGGKSLIWGRACQRWSPYEFTAPERQGYGMNWPINYQDIAPWYSHVEKFVGVC
                     GSRDGLEAMPDGEFLPPFGLNCVETHMRDKIKATYPDRHMVDARWAQITRADEIYLQQ
                     GRGKCQARNMCMRGCPFGGYFSSVSSTLPWAEKTGNLTLRPHSVVHSILYNEGTKKAS
                     GVRVIDAVSKEEMTFHAKVIFLNASALNSLLILLHSKSSRFPEGLGNEYDLLGRYVAF
                     HNYRSYTSGQLDGFEDKYYYGRNPTEPIIANYRNLGKQTMDFAGGYTIFTGAYRSGFE
                     TYEGPQFGPEFKEHMTRPGPWRVYMYMQGETIPKETNRVSLSDSEKDPWGIPLLVTDV
                     GYDDNDDRMVKDFMQESVDMMQAAGCRDISTGDNHQAPGLDIHEMGGCRMGTNPKNSM
                     LNAWNQMHLCKNVFVTDGACMTSTGNQSPSILYMALTARAVSHAVDELNKRNL"
     gene            34561..35409
                     /locus_tag="PKM27_05750"
     CDS             34561..35409
                     /locus_tag="PKM27_05750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNR06802.1"
                     /translation="MDQRIINLYDEYIHKPLNRQDFLNRLTTLVGSSAAAMAILPQIE
                     VDFTHANRTPDADLFTEYITYAGVQGTMRCYVARPKEEKKYPTVVIIHENRGLNAHIE
                     DVARRAAQAGYLAIAPDALSPLGLTFANEDEARTKFRDLKAEDNNANFIRVFEYLKTR
                     KDSNGNYGCVGFCWGGAMANTLAVNVPELKAAVAFYGRQQPAENVPKIKAALQLHYAE
                     MDEGVNAGIVAYEAALKANNIPYELYMYAGAQHAFHNDTAPSRYHEASAKLAWQRTLD
                     FWAKYL"
     gene            35657..36514
                     /gene="hisG"
                     /locus_tag="PKM27_05755"
     CDS             35657..36514
                     /gene="hisG"
                     /locus_tag="PKM27_05755"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNR06803.1"
                     /translation="MTRLNIAIQKSGRMHDDCMVLLRDCGLRIETVSDQLKTSAGGFP
                     LDILYLRNGDIPEYLQDGVADIAILGENTVIEKGSRVTGLLPLGFSKCRLSIAVPQKS
                     GYEDLRFFEGKRIATSYPRTLAKYLRDKGVTAEIHQISGSVEIAPHIGLADGICDLVS
                     SGNTLFQNKLEEKEVILHSQAWVYANNELDADKKALVDQLLFRIKATLEARKNKYILL
                     NAPKASLDKIISILPGIKSPTILPLAIEGWCSVHSVIPEKEFWEVIDALKQAGAEGIL
                     VIPIEKMVS"
     gene            36517..37806
                     /gene="hisD"
                     /locus_tag="PKM27_05760"
     CDS             36517..37806
                     /gene="hisD"
                     /locus_tag="PKM27_05760"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNR06804.1"
                     /translation="MKIYLEPPRVQWAEITRRPSGQDLSQESVVQSILDRVRQEGDTA
                     LKKYTEKFDQVSLQSFGVSQEEMDQAEKKLDQRLKEAIQIAKSNIEAFHRSQWHEPGI
                     VETMTGVKCWQKTVPIEKVGLYIPGGTAPLFSTVLMLATPAGIAGCREILLCSPPDKF
                     GSVHPAILYSARLCGVTRIFKVGGAQAIAAMAYGTKEIPAVHKIFGPGNSFVTLAKMK
                     VQQEGIAIDLPAGPSEVLVLADESADPSWVASDLLSQAEHGLDSQVVLVADSEDFVRQ
                     VGLEMESQLQELPRKKYATGSLAHSHALVLKDRKDQLDFINEYAPEHLILNHRDADAI
                     SNEVINAGSVFIGPYSPESVGDYASGTNHTLPTSAYAKAYSGVNLDAFTKKITFQKLD
                     PEGLQAIGPYVELMAQAEQLTAHQRAVSKRLNKLNRP"
     gene            37803..38849
                     /gene="hisC"
                     /locus_tag="PKM27_05765"
     CDS             37803..38849
                     /gene="hisC"
                     /locus_tag="PKM27_05765"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNR06805.1"
                     /translation="MTIESLIRPNILKLKPYASARHEFTGKGEVFLDANENPFDTGVN
                     RYPDPLQEKLKSAIGRLKNIPTDQIFLGNGSDEAIDLILRIFCVPGRDEIMVLPPTYG
                     MYQVSADIADVQVKKVLLQADFQPDLPSIRNNLGPHAKVIFICSPNNPTGNLIRREAI
                     EEILEFFGGIVVVDEAYTDFCPEATLLPLLEKYENLIVMQTLSKAWGMAAIRLGMAFA
                     SPLLISYFNKVKAPYNINILTQRFALERLSKEGEMKAQVQSIRQQKTWLTRELESLNE
                     VLQVYPSDSNFLLVKVRHAEALYGYLSAQGVVVRNRSREPLCENCLRITIGTETENRT
                     LLEKMNLFQNQAPV"
     gene            38846..39955
                     /gene="hisB"
                     /locus_tag="PKM27_05770"
     CDS             38846..39955
                     /gene="hisB"
                     /locus_tag="PKM27_05770"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HNR06806.1"
                     /translation="MNMQKVLFIDRDGTLIVEPQDNFQIDSLEKLEFIPGVFTWLGKI
                     ASQLDYTLVMVTNQDGLGTPSLPETDFWPAQNKMMTAFKNEGIHFAAVHIDRSFEHEH
                     KETRKPGTGMLKEYLTGSYNLPESFVIGDRLTDVQLAANLGCQAILFNDDLDSAALAV
                     AHQSRSWKSVYEFLRLRSRVVHHQRHTRETQIDIRLDLDGTGKSSISTGLGFFDHMLE
                     QIARHGECDMEVRCKGDLHIDEHHSIEDTGIALGEAFLKALGDKKGMERYAFHLPMDE
                     ADAEVLLDFGGRAWLVWEAEFKREKIGEMPTEMFYHFFKSFCDAAKCNLQVRVRGENE
                     HHKIEAVFKAFAKCIRQAKRRDPASDLLPSTKGVL"
     gene            39971..40567
                     /gene="hisH"
                     /locus_tag="PKM27_05775"
     CDS             39971..40567
                     /gene="hisH"
                     /locus_tag="PKM27_05775"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNR06807.1"
                     /translation="MKQKIAVIDYNAGNVQSVLFALERFDVAAVLTRDPDEIRKADKV
                     LFPGVGEASTTMGFLRARGFDQLIPGLTQPVLGICLGLQLLCEHSEENDTACLGILPV
                     KVKRFVATSHEFKIPHMGWNQLELNQQDWLAPSMDRQYVYFVHSYYAELNPYTVASCT
                     YCQPFSAVIHKDNFYATQFHVEKSGPVGLKILETFLDL"
     gene            40582..41304
                     /gene="hisA"
                     /locus_tag="PKM27_05780"
     CDS             40582..41304
                     /gene="hisA"
                     /locus_tag="PKM27_05780"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNR06808.1"
                     /translation="MIVFPAIDLIGGKCVRLTEGDFERLTDYQADPVDTAKKYEDAGL
                     SHLHLVDLDGARNKKVTQYKILEKICSKTNLKVDFGGGIKTDDDLRIVFESGGAQANV
                     GSLAVSEPEIFEAWMVEYGSDRIILAADVRDMKVAAHAWKETSPISIFDLLDRFEPRG
                     LIYLTCTDISRDGKLTGVNHELYKTLMEKYPALRVTASGGVHHVKDLQGLLEMQCFGV
                     IVGKAIYENKIRLEELVSFQLN"
     gene            41315..42091
                     /gene="hisF"
                     /locus_tag="PKM27_05785"
     CDS             41315..42091
                     /gene="hisF"
                     /locus_tag="PKM27_05785"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNR06809.1"
                     /translation="MLAKRIIACLDIKDGRTVKGINFLGLRDAGDPVELGRLYSDQGI
                     DELVFLDITATHEKRKTLSALARAVAKNINIPFTIGGGVTSVEDASALLESGADKVSI
                     NSAAVREPDLIRRLALEFGSQFVVLAVDGRLTENGWEVYIQGGRLSTGLPMYDWIRKG
                     VDLGAGEILFTSMNHDGMKSGFAVEATRMVSESVSVPVIASGGAGTMEHFADVFREGK
                     ADAALAASIFHYREIGIPELKDFLRTRNIAVRNQPSEVSL"
     gene            42199..43713
                     /locus_tag="PKM27_05790"
     CDS             42199..43713
                     /locus_tag="PKM27_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR06810.1"
                     /translation="MNKGIAKIIGASSVGTLIEWYDFYIFGSLAVLIGKKLFPPDAGA
                     SALINTLAIFAAGFIVRPFGALFFGRIGDLVGRKYTFMLTLVLMGVSTFLIGLIPGFD
                     SWGYAAPLAVLVLRLLQGLALGGEYGGAATYVAEHAPPTHRGIYTSWIQTTATLGLFL
                     SLGVIVYTRYLIGTEAFNDWGWRVPFLVSIFLVGVSVYIRIRMRESPSFARIKAEGKI
                     SVNPIKESFSHKANFKMVLLALFGAVMGQGVIWYTGQFYAQSFLENTCKIEFNQSRYI
                     LLIAIAFATPFFIFWGWLSDKIGRKWIMIAGMVLGVLTYRPIYRYFYEKTNSVVLERS
                     EPVKSTPLPLIRMKNDDGDSVFVNQRIQTLANGISFKQARVDILFRDPEKADRALGQF
                     AIIDKTLPPGLFWIFVFLVFIQILYVTMVYGPIAAFLVELFPTRIRYTSMSLPYHIGN
                     GVFGGLVPFIATLISSFPGSNYLSGLWYPIGVALVCAIIGSLYLENKINVENRD"
     gene            43873..44211
                     /locus_tag="PKM27_05795"
     CDS             43873..44211
                     /locus_tag="PKM27_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HNR06811.1"
                     /translation="MLLTSTNTIEGHKIRHYLGLVTGDTIIGANLVKDFFASITDIVG
                     GRSGAYEKVLKEAKSLAEAEMVNKALSMGANAVIGIDFDYEVLGRGSMLMVSMTGTAV
                     IYDEFAKKEA"
     gene            44208..44819
                     /gene="hisIE"
                     /locus_tag="PKM27_05800"
     CDS             44208..44819
                     /gene="hisIE"
                     /locus_tag="PKM27_05800"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNR06812.1"
                     /translation="MKTDLSQIRYDAGTGLIPAVIQDEMTGKVLMLGYMNREALEKTR
                     STGKVTFFSRSKQRLWTKGETSGHFLHLRSIATDCDDDTLLIKVRPEGPVCHEGFDTC
                     FREINLSADFIQELENIIRDRKANPKAGSYTNSLFDSGVQKIAQKVGEESVETILEAQ
                     APGKEALVYEASDLVYHLLVLLQAKDSSWGEVIDQLKLKHGAR"
     gene            44809..45312
                     /locus_tag="PKM27_05805"
     CDS             44809..45312
                     /locus_tag="PKM27_05805"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNR06813.1"
                     /translation="MPGEHPYQAYLFSPIGYIYLARSEMGLTHVHLSRKEPGDDFSQG
                     QDDLLLEAKKQLASYFSGSLQRFDLPLDFGKAPEFSQAVWRLLLQIPYGHTRSYGALA
                     RDLGDIRKSRAVGHANAVNPMAIIVPCHRVIGSTGALTGYAYGMDAKEFLLALENPNR
                     GKQTELF"
     gene            complement(45403..45951)
                     /locus_tag="PKM27_05810"
     CDS             complement(45403..45951)
                     /locus_tag="PKM27_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNR06814.1"
                     /translation="MTQPSFLSALDLHKDRLLRFAFNLTRNQDDAKDLLQETYFRALT
                     NREKFQEGTNLKAWLLTIMRNLFINNYRKKAKTRVLFDSTDNLFFLNSGSNSTGNQGE
                     SNILMDELTHMVDELDESIRYPFVKHYEGYKYQEIADDMQLPLGTVKSRIFFARQDLK
                     SKIVNRYGNTLGLKERELKMAS"
     gene            complement(46031..47128)
                     /locus_tag="PKM27_05815"
     CDS             complement(46031..47128)
                     /locus_tag="PKM27_05815"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNR06815.1"
                     /translation="MKILDWYIIRKYLGSFFFTAFLFTLIAMVIDFSEKAEKFVDASK
                     VLSLGEILGQYHLNYIPFINGLLWPLFSLIAVIFFTSRLAYNSEIISILNAGVSFPRL
                     LRPYLVAASLLTVILLIANHFIIPIANKPKLEFERRFIKRSSDRLTTGTNMHFFISPD
                     TKVYLRSYSAADSLGYSFRLEKFRGTTLIYMLKAQFLEWNKETRNSWKIRDYEIRTYE
                     DSVETITLHPNQQIDTALNILPSDFEFYSKKKDLMTTPELQALVDKERSRGLNPVKVY
                     INEIQRRTADPFTILILTVIGVAIAGRKVRGGLGLHLALGIALGSVFVFISKFSIVFS
                     RSPNIPSVLGIWLPNIIFAIIAYRLVRKAQQ"
     gene            complement(47125..48264)
                     /gene="tgt"
                     /locus_tag="PKM27_05820"
     CDS             complement(47125..48264)
                     /gene="tgt"
                     /locus_tag="PKM27_05820"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNR06816.1"
                     /translation="MGVSFELKATDPAGGARSGILRTDHGEVPTPVFMPVGTAASVKG
                     VSPEELKKPVHARIILSNTYHLYLRPGTGVLQAAGGIHRFMQWPGPVLTDSGGYQVYS
                     LSKLRKIREEGVSFSSHIDGSRHLFTPEAVMEIQLQIGADLIMAFDECPPYPCEYPYA
                     KQSMGLTHRWLERCFSYFNQASLPYDYQPALLPICQGSVYQDLRTESAKFISGFEAPV
                     YAIGGLSVGEKNQDMYEMIRLVNQYLPASRGRYLMGVGTPENILESIALGIDFFDCVL
                     PTRNARHGLIYTTEGILNIKNQRWKFDQSPIDPGLPETYSQPFSKSYLRHLVQSNELL
                     GARIASIQNLSFYLYLVDTARQKIEEGIFTPWKNQMLEKIKTRLN"
     gene            complement(48269..49684)
                     /gene="rodA"
                     /locus_tag="PKM27_05825"
     CDS             complement(48269..49684)
                     /gene="rodA"
                     /locus_tag="PKM27_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNR06817.1"
                     /translation="MSSIISRNKEFAKVDWITLATFLSLVVIGWMMIYASGYSELTES
                     TTPFFRTNAGKQLIAIIISAFLFLLVFITDFKFWSSLSYPIYLLGLLFLVAVLFFGQK
                     IKGSTSWFNLGGFSFQPSEFAKFGTMLAMAAYLSYFKTNLKQFKSRLISMMLFAVPIG
                     LILMQPDAGSAITFGSFFVLLFREGFNPVWYIIGLALLALFILALKFDPLYVIAYILA
                     IGSTLIYSQLRKNNQQLFIVLASWGLIMASYWSKVPIMAVLGLLGTGLAFFIYQGYQQ
                     RLQRLLIILVPSILAGAAFTKVSQYTVDNFLKPHQRERINVWLRPEMCDPRGNLYHVL
                     QSKLAISSGNFSGKGFLEGTLTKLNYVPEQSTDFIFSTIGEEQGFLGIIAIIGLFVLL
                     MLRAIAIGERARNTFTRVYAYSFLGFILVHFFINIGMTMGLVPIMGIPLPFISYGGSS
                     CIVFSLMTAVLLRLDSSRHFV"
     gene            complement(49681..50082)
                     /locus_tag="PKM27_05830"
     CDS             complement(49681..50082)
                     /locus_tag="PKM27_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNR06818.1"
                     /translation="MRLSMKLMLMLCFAYGLGQAQTGRLQVIESPAITRLNDYFIQYN
                     LKRADFPGWRIQVFASTDRREMESALSNFRSRFPQYSSKWLLNDPYYQIRVGVFTEYS
                     EAMRSLNEIRKTFRSATLVSDRIRREEVLNL"
     gene            complement(50106..>50678)
                     /locus_tag="PKM27_05835"
     CDS             complement(50106..>50678)
                     /locus_tag="PKM27_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNR06819.1"
                     /translation="ENWDLKGLDDALFKQYHLHLNISGAQELRSPEDIKSFLNENVLK
                     HLRNKEKEFGPELMTYLMKVIMLQAIDTHWKDHLLSMDHLREGIGLRGYGQKDPIREY
                     QREGYAMFMEMIGTIKMDTLEKLCLVRIQREEEVEEIRQKQKQDYILSRGEDTPAHQT
                     VKRATEKIGRNDPCPCGSGKKYKQCHGANA"
BASE COUNT        12450 a        12920 c        13006 g        12302 t
ORIGIN      
        1 tgatccagga cctcgaccgc ggggtggtca tgggccagca aacctatgga aagggcctgg
       61 tgcagaatac ccgggagatc gggtacaaca gccaggtcaa actgaccacg gcaaaatatt
      121 atatacccag cggccggtgc atccaggccg ttgaatatga aaacggggaa ccgaaaaata
      181 tacccgattc cctgcgggcg gaattcaaaa ccaggggagg gcgcaaagtg ctggatgggg
      241 gaggaatccg gccggatgtg gtgatcgatg ccggtaaaca acctgaaatc ctgaaacaga
      301 tgatcgaaaa acagctgccc tttaagtatg caacccatta caacctggca cacccatcga
      361 tcaagccggt tcgggagttc agttttacgg aatacgacga tttcaagaaa ttttacaggg
      421 aatccggctt tgattttact tcttctgccg aatcggccct ggaggcgttc cgggccaaag
      481 cgtccacgga ggaatcctgg tccaggatcc gggaagatgt acaggccctg caatccaaac
      541 tcatccagga tccgggcaaa ttgctggacg aacaccggga cctgatttca aggcatctcg
      601 agaaggagat catcccgaga tattatttac aaaaaggaaa aattgagaag gctctccagg
      661 tggatatgga gatccaggaa gccataaggc ttttgggcga ccccacccgg tatgaagcct
      721 tgctgaaaaa aaaataaaat gaccccggat gcccttctcc tgcatattga aacaagtacc
      781 gaggtctgtt cgatagcgat cagcaagggc ccgcacctgc tgtcaaacga acaggagcgg
      841 caggggagaa agcatgctca atggctgcct ctgatgatcg aaagcgccag gcagaaggca
      901 tcagtgggcc tcaaagacct ggatgccgtt tgttacagca ggggccctgg ttcctatacc
      961 ggtctgcggg tggggctgtc cacagccaaa ggcctttgtt ttgggctggc gaagccgctg
     1021 atcggcgttc caacgctgct ggccatggca catagcgggg gggagcccgg acctttttcc
     1081 ttgtttgttc ccatgatcga cgcccggcgg gatgaggtgt atatggctgt ttacggcccg
     1141 ggatggcagg tgatcgcggg gcctgaatcc ctggtgctga gccccggtag cctggatgaa
     1201 tggttggaaa agggttattc cgtccacctg ttcggggatg gctctgtaaa ggcaaagacc
     1261 atactaaagc cccatgaacg gttaaatttc ggacccgccg aggcggatgc ccggtacatg
     1321 atcaagcccg ggcttgacca atttaatttg agccggtttg aaaatctggc ctatgctgtt
     1381 ccggaatatc ttaaagttcc gaattacaag aaaacgttaa taaatacctg aaaaccagat
     1441 gaatattctt acatatataa ataaagcata taaatatttt ctcccggtta aaatacattt
     1501 gggtcatttt caattcaata gataaaagtt ggtacggttt tggtttaact ccgtgtattc
     1561 aaacatcatt tgaaccataa acctgaggag tatgaaaaaa caaaaacaag aaattgttac
     1621 gctgaagaag ggccccaaag acattcagct cgactacacc gatctgcgcc gtgccgtttt
     1681 ggttttacgt gcggtaaacc acaaattgcg gcagcgcatc atcgaagtac tggaagaaag
     1741 cgacaatatg accgtgacgg atatttatat caaactcagg cttgaacagt ctgtggcctc
     1801 ccagcacctg gccatcctcc gccgggcggg cgtggtgatc accgaccgcc agggcaagtt
     1861 tatctattac tccctggata aagaaaggtt gaatcaaatt tcaaaattga tcgaagagct
     1921 agtgggctaa atcaaagaat taattatatt attttataat attgaaaacc aatggattac
     1981 atccattggt tttttgtttt taaggccatt tttagcccgg attgcttaca aataaagtag
     2041 aatgaattac aaataataaa tttttttaat attttctttg tggtattaaa actaattatc
     2101 atatttgttc tgctagatat tcattgacct actaaagatg taattatgaa gaagactaag
     2161 gttacattcg atcaggaaaa actacaatat tcctctgaac tgctccgggc ccttgctcac
     2221 cccctccggt tgaaaatttt agaattcatt gaccgccacg aagtgatcaa tgtaaacaag
     2281 atttacaact ccctgaaaat agagcaaagc atcacctcac agcatctgaa aatcctaagg
     2341 ggtgccgggg tggtggacaa caacaaagac gggaagttca aacattacca gatcgattac
     2401 cagaaagtag ctaatgccgt aaaggcaatc aacaacttta tgggaaaata aaatcccccc
     2461 cctttttata tcccccccaa tctaagctga agacctcagt ggttttcagc ttttttcgtt
     2521 ttttaaaacc ggtctgcctg ctctgttttg cgggtccgtt tggagaaaat caagcctctt
     2581 ctgttttatc cccttccggc tggtcttgat cggtcgcctg gagatccggc agattctggt
     2641 cgtcggatgc gacctccggt atcagggcgg aaggttctgc ctgcttctcc tgtccatcca
     2701 caacaaggct gtcttcagcc cgggcttcgg gtttggcatg gatcatttct tcatgcgggg
     2761 ctttgaaaaa actccggaag aaaagaagga gggcgatcac cccggaagag atggcggcat
     2821 ccgcgacgtt gaataccggt ctgaaaaact ggaaagattt gccacccaac aggggcatcc
     2881 attccggcca gtacgtatcg atcagcggga aataaaacat gtctaccacc cggccaaata
     2941 aaaacggcgc atagcccttg gatggattgt attcagccag gcctccctgg taataggact
     3001 ccgtgaaaat aaggccgtaa aaagcggaat ccaggatatt gccgatcgcc ccggccagaa
     3061 taagggaaaa acagaccagc aggccggtgg attcccggga acggaccagt ttataaatga
     3121 tgtagatgag aaagccgacc gccaggatcc ggaaacagct caacaggagt tttcccgtat
     3181 tgccgcccag ggagatgcca aaagccatgc cttcgttttc cacaaaatgg attctggccc
     3241 aatccagtcc caggatcctg aattctgaac catattccat attggttttg acccagatct
     3301 taagccattg atcgagcacc aggacgatca ggacggtaaa aatggtaatc agggaccttc
     3361 tttgaatcga agaggcgctt cttatggttc cttccatgga ggggcaaagg taggacgaaa
     3421 tatgaattgg aggggaggat ttgggttggg gataggtccc gaggacttgg gagaggttga
     3481 ggttgaggtt gaggttgagg ttaaggttaa ggttaaggtt gaggttaagg ttaaggttaa
     3541 ggttgaggtt gaggttgagg ttgaggttga ggttgaaaaa gaaaagccag ccggagttca
     3601 ccagctggct tttgaatatt ttgggtacag gatattattg tttatcccac catacccggg
     3661 tatccaggtc gtccgctccc tgtctggaga tggcttcctg aacattggtg ctgttcacgg
     3721 aaatctccga gttgggatag gtcagcctgc ggataaaatc gcccttgatt cctttaccag
     3781 gaaagggatt cggcatcagg gccggataac cggaccttct gaaattggca aaggcttccg
     3841 gtccgttcat aaaagatgaa atccagtatt gggtattgat ttgctccagt ccttttgcgg
     3901 gatcataagg gttggcagcg atatacgcat ccgcctcttc ggtagggaca gcggaattgg
     3961 catcgtgaat cgcgcattga tccatatggg ctttaatccc gtttttatag gaatcggcgg
     4021 cggatccgct tgcccaaccc ctctgaatgg cttcagcgag caagagagag gtttgcgaat
     4081 aggtgacaaa aaacatggga gccgtatttt tcccaaaacg ggtgcgatcc acctggctga
     4141 aatcataaaa actggccagg cccctcgcgg ccgcctgggc ggcaattcca ttattgtcat
     4201 atccgaaagg catgccaatt tgtacggcag tatccctgtt ggccctggcg gcggtctgtt
     4261 cgggtcctga tttggctcct acatagcgga cggcaatagc cttaagcctg ggatctttgg
     4321 tccttttgag gtgatccaaa aacggggcgc cgatgtaaga gttcgccgct tcagtactgt
     4381 tcatccattg tccgatggca ttggtataat tggcatcgtg ccgggtatag gcattatccg
     4441 cattggaggt catcaatccc ccggctaccg ctttttgcac cgtggcctgg gccagggtgg
     4501 gatccacttt tgtaaggcgc atacccgccc tcagcaataa ggaatatcca aactttttcc
     4561 atttagcgac gtcacccccg tacatgatgt cggccgtttc gatgggtttg gaagcatcca
     4621 gggcggcggt ggcttcggtc agggttttga tgatgtcggc ataaatggcc tgctgggcat
     4681 cgtatttggg cagggtgatc tgatcgctgt atcctttgcc ggcatcaaaa tagggaactt
     4741 caccatagga atccgtcaag accatgaagg caaaggattg aaggattttg gccatattca
     4801 gcaggttgct gcggtttggc tgttcagagg tttggcgaat cacatccgtt gtatttcgga
     4861 tgacgttacg gtacccccgg acccagtttt gattggtcgc gtcccggttg tcctggttga
     4921 aattggctcc ggtcaacacc cccgagtttg gggataccat ctgctggacg atgccgatct
     4981 cataaatgat ggtcacgttg ctgtaggagg cgctgatcac cgcgttattc aggataaaag
     5041 cggggttgat cgccgtggcg gcattcttat tgatgttcag ttcgtcaaag ccgtcgtcac
     5101 aagaaacgat caccaggctg agtgtcagga atagaagttt tatataattt ttcatgatgc
     5161 gtttcatttt tagaatttga cattaagatt aaaaccaatg cttctcgtgg tgggcaagcc
     5221 cgtggattcc attcccacca ggttatcgga agtatatcca aaggattcgg gatcgatgtt
     5281 gggcacccat tttttaagca tcaacacgtt gttggccaca aaactcagct taactcctga
     5341 aatcggaaca gagctgggga tgaatttggt gagatcgtaa ctggcggtga tttgcctcag
     5401 tttccagtat cctccattgt agatgaccgg ttcgatcagg gccagggagc gcacgacctc
     5461 ccaataaggt tggatattgg ccttgaccgt gttgacctct cctttctcat ttactccgtc
     5521 accgacgata ccatcgcggc ctgccagggt ctgcttgtgc agaccatgcc tggtcaggtt
     5581 gaaattggta cctgacagca ttttattgcc cagtttaaag tcgatcagga aggaaaaact
     5641 gaaatcattg aatgtaaatc cattgttgat ccctcccgtc cacttgggca aagcagatcc
     5701 gaaagaaatc agatcgaggg tgcgcagggc gatcccgttg ccgccgaaga tctttcggcc
     5761 ctgggcgtcc cgccggtatc cgaaaccata aatctgcccc atttcctgtc caacgacctg
     5821 cctgagttca ccgttgaata cgtgggtgcc tacggtaatc ctttcccctt cggtgtcggt
     5881 aagcagtttg aggaccttgg ttttattgaa agatccgtta aacgtgatat cccactggaa
     5941 accaccgctt ttaatcggaa cgaggtttaa taatccttcg aatccgttgg tgcggcttgt
     6001 cccgctgttg atcagggtat tggtgaaacc cgatccgtcg gaaatctgtg ccgaaacgat
     6061 ctggtcctcc gtaattttct ggtagacggc aaaatccagg gcggcccggt tttggaataa
     6121 ttttaattcc aggcccgctt ctttttcaga ggtgcgcatg ggccggagat tggcattggg
     6181 taaaacatta cccgaagctc cccctatggg ctggagcgcc ccggacgggt tcgggaagag
     6241 gttggcattg actccgtaaa ataaattgtt ggcgtaagga cctacgtccg tatccgaacc
     6301 tacttccgca taagccagcc tcagttttcc aaaactgagc cagctgggca tgccgctgaa
     6361 agcatctgaa aacacaaaac ttcccgaaac ggaaggataa agtatgctcc ggtttgccgg
     6421 tgaaagggtt gaaaaccagt cgttcctcag ggttccgttc aggaacaaga tccgtttata
     6481 ggagacgtcc gctgaaccgt atattgaatt tacggcacgc tcggacaggg aatagatcgg
     6541 atctttcacc cggccattgg ctactgtata taaaccgcgt acgacgaagt cctggacaaa
     6601 tacattgttt tggtccgccc ggcggtacat tttgttgcct cctgcagtaa gatcaattcc
     6661 aaaatcgccg aattccctgc tggcggaaat caacaagtcc atattggtct cccggaaacg
     6721 gcgggattcc tgggtaaacg atccattgac aaaccccacg ggtgcggcag ccaatgaggc
     6781 atgaccggtt gggaaattgt tgtagtcctg gtcgcgggtc cagtagtcct gtcctgcccg
     6841 gccctggatg ctcaaccagg gggtgaggtt gtatttcagg gtaaggttgc cgtacaagcg
     6901 gtcccggacg acatggttga actgttcggc caaggtccaa taaggattgg tccggttcat
     6961 aaacctggaa tagataaatt catttccctg ggcattgtat tttttagcat ccagtaattc
     7021 cagcggcatg ctgttggcca ggttgtataa ggaagtcgga atggagttgt cctggttggc
     7081 tatgtttgga gggttgttgt ttttttcttt agaataattt gcgcttcctg aaaaactgag
     7141 ttttttcgac aaatcatagg caaaacccag gttcagaatt ttacgattta aactgttgtt
     7201 agaaatgatg ccctggtttt ccgtattatt aaaagaaaga ttgaaacctc ctttttcgtt
     7261 gctggatgaa agggtaaggg tgttggcaaa attctgtccg ttccggaaga attttttgat
     7321 ccgatcgaac accggctcat aaggcaccgt tacattatca aataaaacct gagtcattcc
     7381 cggttgaaat ttttccccga aagaccattg tccggaagtc gggttgggtg tagtgggccg
     7441 caccccgttt tcaccctggc catattcgta ctgataatcc gtaaaatcca atggagtgtc
     7501 gttggtgtaa ttggaattga aggttacccc gatgcctttc gatttggcat cgccgcgggt
     7561 cttggtggtg atcataatga ccccgtcctt ggccctggat ccatataaag ctgccgccgt
     7621 ggcccctttc agaatggtca tgctttcaat atcatccggg ttgatgctgc tcaaaccatc
     7681 ccccccatcg gaggtgaccc cgccgccccg aaggcctatg gaattatctg cgccggcatt
     7741 gccgggattg tatccaaaat tggtgttgtc aatgggcacc ccgttaataa caattaatgg
     7801 attgttttgg cccgcgatgg aagattgacc gcgtatcctg attttcgaag ttcccccggg
     7861 gcccgtaccc agggaagaaa tattcacccc ggcaactctg ccctgaaggg agttgataaa
     7921 gttgggggac ctgttttccg caaccagttc cttggtcacc gtactggtag catagcccag
     7981 ttttttagcc tctttgcgga tacccaggga ggtcacgacg atctctccca ggctctctac
     8041 ggtggattcc atagtcactt ccagattcgt ctggtttccc accgctacct cgagggtctg
     8101 ataacccaca taactgaaca cgaggatgct ttgaggagtg gcctcaatgg aaaaccgccc
     8161 atccacatcg gatacggagc ccgtattggt gcctttgatc aggatggtga cgccggtgag
     8221 gggggacccg tcttcccctg ccttgaccat accggtgatg gtctgcgctt gcagtatgcc
     8281 cgcaacggac aacagcagca caaaaaataa taacctaagt ttattcatat ccttgatttt
     8341 agttttttac aattcgagcg gttgcggcgc catgccgcca gtcagggata gtaaatgtaa
     8401 acgattgtag ataaattgaa aagcatatat gaaaaaatta tttatatagt atgcgaatga
     8461 aattaccggc caagcggtct ctttttcgta ctccggatcg ttttttcatt atggtttttt
     8521 cactgccagg ggttatgcaa ggcttgacgg tactattttc cgtcctttgg tgttcagtca
     8581 ccgctgatgc ccaggtccgg atgcggacgg tctatgccgg cggcatggat ccacagtcaa
     8641 gggattccca ttccgtattt atggatcctt tggtcaccgg aaacaggctg ctcctggttc
     8701 atttgcctgc cagttttgac aagaccggcc gctcttcggc gttttgcacc ctggcggccc
     8761 aatccggtta tcgggtcatc agcctggctt atccgggcat ctcaaacatg tacagcagtt
     8821 gtgaacactc cacggacgcc aattgctacg agaatttcca ccttgaatta tttgatggga
     8881 acaaccgggc cccccagatt gagattgact ccggcgaaaa catccttcac cgcctggtaa
     8941 atctgctcca agtattggaa ttgaaatacc ctgaagaaga atggaaccag tttttagagg
     9001 gaccggacca gatcaggtat tcctccacca tctggacagg acattcggac ggcgccggcc
     9061 acgccctggc cgttgcgaaa tatcaccagg tgcaccgggt gatttgtttt tccggtccga
     9121 aagattttaa ccttcattat tacctgcccc cggcctggtt tcacagcgga ctatggaaaa
     9181 cggggaaatc ggagatcttc gcctttgccc acaccgccga tgaatatgcc ttccagcgtg
     9241 aaatctggga cagcctcggc ctgggcaaat atggcccccc ggtgaacgta gaaaccaacc
     9301 cgcctcctta tctttcttcc catcaattga tcagttccca atccttgcct gcggggcata
     9361 tacacggttc gaccattctc gatgaccaga tgcccaggaa ggatggcaag tccctttatg
     9421 agccggtttg gaaatattta ctggatatcc gatccacccc ggtggcaaac ctaaataccg
     9481 cttcttcgat tgccgtatat cccaatcctg tacaccgcgg gaattcaata ttcctgaatg
     9541 cagatattaa aaaccctggc ctgcatttgt tcgatatcca aggccgcctg ctccggatct
     9601 tcagggatgg gaaaatgtat atagatcagg atttgcctgc aggcccctgc tttatacaat
     9661 ataataacgg aaaccgctta caggtcattc gtctgaccat cctttgaatc aaagattcaa
     9721 aagcaggctg accgggtctt cgagcaattt tttcaccttg actaaaaagg tgaccgaaga
     9781 cgaaccatca atcacccggt gatcatagga aagggcgagg tacatcatgg gcctgatttt
     9841 gatctcccct gccaccgcga cgggacggtc caggatggcg tgcatgccca ggatggcgga
     9901 ttggggttca ttgagaatcg gggtactcat catggaaccg aatatccccc cattggtaat
     9961 ggtgaaagtg cccccgctca gttcatccag gctgagtttt cccgcccggg ccttgtcggc
    10021 cagttccttg atcttaaatt ctatttcatg gaagttcagg gattcgacat tttttaccgg
    10081 gggcactacg aggccattgg gggtggaaat cgcgatggag atatccgtat aatcatggta
    10141 tacgagttct tccccgtcca ggaaggcgtt cacttcgggc atctccagta agaccttgct
    10201 gcaggctttg gcgaagatcg acataaaacc gagttttatg ctgtaccggg ccacgaaggc
    10261 atcctggtat ttattacgca aggccatgat ctccgtcatg tccacttcat tgaaggtagt
    10321 gagcatggct gtttcattcc tggcgctcac cagtctcctg gaaatggtgc ggcgcatacg
    10381 ggacattttt tcccggcgtt ccgaacggga aaaggaggcg cttggtttgc ccggttcaag
    10441 ggaaacgggt ttgtccgggg cctgcccggc cttggcttcg ctggggacgg gcttggtttc
    10501 ctgttcccgg gaaacggcct gtaaggcatc cgccttggtg atccggcccg ctttgcctgt
    10561 gcctgcgatt tcagtggacg ggatgccttg ttcgcggatg attttagccg cggccggcga
    10621 agcgatgggt tcggacgatt tgttttcccg aactgcggaa ggggcgggag tttcggtttt
    10681 tgccggttcg gcagcctgaa ccggggaagg cgcagcgctg gtatgggcgg tatcgatttg
    10741 agcgatcacg gctcctattt tcagatcgga gccgtccggt accaggatat gcagtttgcc
    10801 ggcggcttcg gcgggaaggt ccagggtggc cttatccgat tcaaattcac agatgggttc
    10861 gtccattttg accaggtcgc cttccttttt tagccatttg gaaagggtga cctcggtgat
    10921 ggattcaccc atgttgggta ccttgatttc gatgacgctc atggtgcaaa gataagttcg
    10981 aacaggcaat ccggtgaggg tcaaaatccc tgtgggaagc ccggtgcaag cattataaat
    11041 tatcaaacat tcaatctatt tttactcaat gaagaattca gtccggatac agctttctgt
    11101 aatgatgttt ttgcagtttg tcgtatgggg ggcctggtat ggccaattgt ccaagtattt
    11161 gttcgccatc aatttcacgg gggcccaggt gggtaatatt tatgccactt tttccatcgc
    11221 gatgatcatt tccccgtttt tcattgggat gctggcagac cggtttttcg ctgcccagaa
    11281 ggtcctgggc gtcctgaata tcctgggcgc ggtactgttg tacgcactca cccagatcaa
    11341 agagtacaac gccttttatt tcgtgatgct tttatactgt ctgacgtttg cgcccacgat
    11401 cgccttgacc agttccattt ccatgaggca gatgaaggat cccgaaaaag aatttccacc
    11461 catccgggta ctgggcaccc tggcctggat cgccgtcacc aacctggttg gatttatggg
    11521 ctggggagat aaggccacca tcttttatgt ttccatgatt accgccttgg gcattggcct
    11581 cttttctttt accctgccta ataccccgcc aaccatcagc gggccggtca gtgtgggcca
    11641 ggtcatcgga aaagattcgt ttgtgttgtt taaggatcgt tcattcctga tctttttcat
    11701 tgcatcgatc ctcatctgca tccccttatc gttttattat gccctgggta atccgtccat
    11761 cacggattat tacaaggccg ccttcatggc cgccaacccg ggtcaggccc tgcccctgac
    11821 cttttttgta gaaaacaaaa tgtccctcgg gcaggcctcc gaagtgctat ttatgctgct
    11881 tctgcccctg gcttacagcc gttttggcat taaaaacatc ctgatcattg ccctggttgc
    11941 ctggatcttc cggtttgtat ttttcggcta tggcaatgcg ggaagcaatg aatggatgct
    12001 gtacgccggc atccttctgc acggcatttg tttcgatttc ttttttgtaa gcggcatgat
    12061 ttataccgat aagaaagcag gcgacaaaat caagtcccag gcccagggcc tgatctccct
    12121 ggccacctat ggagtgggca tgttcatcgg ttcggtcctt tccggagtgg tcaaggataa
    12181 gtacactacc ggcgccgggg cggacgcgct gaccaattgg accggggtat ggctggtacc
    12241 ggcagggatt gcggcggtgg ttttgatttt cttcttcctg ttttttaagg ataggaatcc
    12301 ggcgcctgtg aaacaattag tcagttaacc agttaaccag ttaaccagtt aaccagttaa
    12361 ccagttagct agttagctag ttagctagtt tacaagtaaa ccagtttgca aataaaccag
    12421 ttggacagta aagcagttgg ccagctggcc agttcgctcg gtcaggacag tttaaccacc
    12481 atttaacccc ggtttaaccc tacttaacat tgaccttcag tcaaattagt acagtttgat
    12541 tttaaaccaa taaaccactg taccatgaaa accaggattt ttaccagctt tcttttttcc
    12601 ctctttaccg tagcgatcgt tgccggtgac cttgatggtt actggtactg cgcagaccac
    12661 cattcaaaca ttgaagtcat gtacaccacg aagagcatca aggtcaggaa tcccgaccgg
    12721 gaacctgacc actgggaatt atacaaccgc tttgaaaaca accggtacag ggacaaccgg
    12781 ggcaatgtat attactttaa cggctcaggc ctggaatgga atgctccggg cagggggatt
    12841 gcccatcatt atacccggaa cagcagtcct tccggcggga attggaggga tccggggcgg
    12901 aacgaccggg gttggggtga ccgtcccgga aatgcgggag gcggatggaa tgaacgggat
    12961 aggggtggct ggaccaatga ccgggatgga tggaacgggt tctataaacc ttatgaaggc
    13021 aagtggcaca atcattccac cggcacccat atccatgtag acctgagccg gcgaagcctc
    13081 cggattaaat tccacggcga aaaatggcat gaggtttttg aacgaaaccg aggtttattt
    13141 gttgatcgca aaggaaatga atttatcttc cggggagagg gcatcgaata ccgggcccat
    13201 gagggcgacc tgatcatgaa attttacaat gacgaccgtt gtttccaccg ggacgattac
    13261 cgggcggaat actggaggta aaacataaga ctgctctaaa tccatagcct ggaccggttt
    13321 tccggtccgg gctattttcc ttgtacggac atcaggatga tatcctgatc atgactatgg
    13381 cctggaatcc ctgtgtttct tgaggtaata aaacaaacct gaccggatgc ctaaccaaat
    13441 cccgccttgt tggtccaacc ccaggctttg atatgcaatc gccccattga ccataatatc
    13501 cacgtatgat aaattccagc ggtcatcccg gttcttggga ttgaaccgga tataaaccgg
    13561 gtaaaggtca aggtcctcag tattaatctg tggcttatta gggtcattgt aagcggcatg
    13621 cagaatattt gctccactgc ccagttggta tactttggaa gcgttacgct caaagtcatc
    13681 gttactggta tccaggtaga attcgcgtcc acccaggccg acatacacgt cgccatcggt
    13741 acctgctcca tcgaagtcac cggtgtgcag gttgatactt aaagtgctga tctgtgacat
    13801 agtctgaagt ttttaatgag ctgataggat ttttaagggt tatgagcaaa agggtcaaaa
    13861 taagatgtaa tacgccctta aaaggtcgga ggcatcaacc caaatttttt tacctgactt
    13921 cagattgtcg tgaacttatt tctttgaacc gggcatttat tatcgatcga cagccaccaa
    13981 tcggcacacc cgggaaaggg tattcgaact gattgtatag gcttatggtg gccgttatcc
    14041 cagtcgttcc ccttcgtacg actgcctttc cttttcgaca aaatcctccc ttaccggata
    14101 aaaacaatcc aggaataagg ccttcgtgag ggcctttccg gaatgaggca cattaccggg
    14161 tatgaaggcg gacattccgg gtttcatgtg gagaatttcc ccgccgatat tgaactcaaa
    14221 ctccccctcg atgagatggc tccattgttc gtgcggatgc tggtgcgtgg gcagcacggc
    14281 tccgtcatcg atggtgaaat atccaaacaa caattggtcc gaataggcaa tgggtccatt
    14341 gatgccctcc catatattta agggcgccat ggatttaaaa tcgataaaag gcatttttca
    14401 attcaattaa aaaatgaaaa taattaaaat aattaaggag gaggcccagc ccccgccttc
    14461 tgcttaccac caagcaccaa ccaccaacca ccaatcacct caatccttaa tccccttcgc
    14521 tgtccggtcc tcatagggcg gcctggattg cattttccgg ggatgggtgg tgatcagttt
    14581 catggtttcc tctaccgcgc gcaacaactg gggatccttt cccctggcca aggcagcagg
    14641 atcatcccag acttcaacgg tcggttcgat gccatggcct tctgcgaacc atttcccatc
    14701 ggtgccaaac aacctggcgt ccggcacggt gatcgtgccg ttgtcgatga gttgatgtgt
    14761 cgtagcaggc cccaccaaaa tacccagggt cctttctccc acaatgggcc ccattttcaa
    14821 ctgctgaaaa ccccagggaa aggcatcccc tcccgacccg gcccagccgt tgatcagcat
    14881 caccttgggg ccgtcgttgg ctttggtggg tatgggcaga tcgtaaccgt ttctccagcc
    14941 aatataataa acggtaggcc ggttgagcat ctccaggaag cggtctccca actggcctcc
    15001 cgcgttaaac cgttcatcaa tgacaaatcc ttttttatcc acctgggcat aaaactgccg
    15061 aagcagttcg gtttgaccct gagtggtggt attgggcata tacatatagc cgagattgcc
    15121 gttggaagcc aggtccacgg ccttacggtt ggattcgatc cattcgagat ggcgaaggcg
    15181 gccctcctgc tggggattca gggtttccac aatgacttct ttcgcttccg ccgcattggc
    15241 cgttttgccg atccgcaggg aaaccacccg ccctgcgagg ccttcaaagg cggcgtaagg
    15301 atccctcgcg cgatcgatgg ttatgccgtt aaccgaaaaa atataatcgc ctgcctttac
    15361 atcgatgccc gggcggtcaa agggagatct cacctcattg tcccaggctg caggacggac
    15421 aatccttttt attttatatg caccctggtc cagggcccag tcaataccga gaaagccggt
    15481 aggacggaat tccacttgtt ccatgtcccc tcccccggca taggtatgcc cggcgctcag
    15541 ttcggagatc agttcaatga gaatattggt cacatccagt cgcgtgatgg catcctgaag
    15601 gagacttcca tagcgggttt tcatcgcggg ccagtcgact tgttgcatac cgggatcata
    15661 aaagaaatcc cggtaccttc tccaggtgtc gttgaacaac tgggtccatt ctgccttagg
    15721 aaccagggac atggtcattt ctgcggtgcg caaggcttta tcgattttct ggtccggatt
    15781 aattttaatg atgccgatgc ggtcattatc ctggacgacc aggttggatc cggtaggggc
    15841 gagcacatat cggttggcgt tggcataaat cgatttttcc tctctttttt ccaaatcgta
    15901 aaactgtacc ggggaaatgt ttccgcccgc accggtattg ggccggcggt ggtaaatgat
    15961 ttttccatcc acggcattga gattgcccag gtttccggcc ggaacgggca ggatcaccaa
    16021 acggctttct gcattttcga gatcgatgat gagggaatcc ctggtcttgc ctgcctccgg
    16081 ttttttaacc tgggagctat ccgtccccgg ttttttctcc tcggtaattt tgatttcatc
    16141 gtttttgggt gcgagggggc tcgaggtggc actttgcagg ctcaatgccg cgatctgggt
    16201 ggtgtttgga tagatccagg tagcgtccag gctgctgtag gagggttgga aagaccggtc
    16261 ggtgaaaaaa tagaggtatt tcccttttgg atcaaacacc ggttcataat cattgtaata
    16321 cccggaggtc agctggctca cttttttagt ttccaggttg tagacaaaga tggcaggatt
    16381 ggtattgtcc gtagccttat aataggtaat ccatttattg tccgcggacc agttgatccg
    16441 gaatccattg agattccccc agaccatgtt gcgggtatgg tctgctatgg tcacttcttt
    16501 cgtggccagg tcaatgattt ttatggcctg catataatca atgagggcga tttttttgct
    16561 gtcgggggac cagaagagcc ggaagccgta acctccaccg aatttactga gcggttcgtc
    16621 cgctgatttt ccatccatgg ctttcaggta gatttcattt tctccggacc ggtcgctcca
    16681 ataagccaca tattttccat ccggggacca ggcgggcatg cgttcaaagg atccgctggc
    16741 attggtggta ttctgaacgt agccgtgttc tgagggtata ttaaagagtt cgccccgggc
    16801 ttctatcatg acgcgttttc cgtccggcga ggactcaaaa ttccggatca tattgttgac
    16861 gcggacctgc ctcggcatgg tggaggccag gtcggcgacc acattgatct ggacttccct
    16921 ttgcctgtga ttgtcaaggt tcagaaggta cagtttcccg ccggcctcaa aaaccagttc
    16981 ttcaggccct atggacatgt gattgatatc atagtcggta aactgcgtga tttgttgacg
    17041 ggtctgccgg tcggtatggt aaacccagat gttccgcctt ttttgagtgc cggcatccga
    17101 tacgtaatag atactgtttt tgtgccaggc cggtttgcca tccgtggcgc tgtttttggt
    17161 aatgttcagg gcggtttgat ttttcaggtc aaagaccact acatcggaag ccatgccccc
    17221 ccggtacctt ttaaagggat agttttcagt gatccgggtg acataagcga ttttcgagcc
    17281 gtcgggagaa taagaaccca gttccccgta agggatggat aatttatcgg gaagaccacc
    17341 tatcaccggc agggaataga actgacgata agccggagtg ccgctttctt tgcttgaagc
    17401 gatcaggagg gaacggccgt ccggatgcca gtccaccatg cggtcaggga aactgttgta
    17461 ggtcaaccgg tccactccgc cgccttgcag ggacatgagg taaacatcgg tattgccgtc
    17521 gtaattggca ctgtatgcaa gcgatttgcc atccggggaa aatcggggaa aactttcttc
    17581 gcccggggag gaggtgatct taaaggccag gcccccttct ttgggtacca gccagatatc
    17641 ccctccgtaa acaaaagcga tttgtttatc agagacatcc ggataccgga ataagcgggc
    17701 atccatctgg gaccaggcgg agaaagtgga taaaacaagg aacagaagta atgggatcct
    17761 gattttcatg tggatgattg agttgtggtg atataaaggt tgaggttgag gttgagattg
    17821 aggttgagat tgagattgag attgaggttg aggttgaggt tgaggttgag gttgaggttg
    17881 aggttgaggt tgaggttgga gcggaaagaa gaggttcctt ccaatccaac ctcatttcaa
    17941 gataataagg ggtttatact ctaaaataac ccactcaggg ggaaagtttt gcaataaatt
    18001 cgactaattt cggcgccatg aagccaaact gtctgattct atggtttttc tttgccggca
    18061 cctgcctggc ccaacaaccc ttgaaaccca ataatccata caccggccct gggactgctc
    18121 cggtgcaacc aaccgtggtt caatctgagg aagaagcggt catcacggtc atttaccgat
    18181 tgtttgatgc catgaaagcg gcggactcgg ctgcagcgag gaccttattt acacccggcg
    18241 caaggttgta caccccggtg accaaggcct ccggcgagac gaccttgcag gaagaaacgg
    18301 tagctgcatt cctcaaatca atcggtacca agcggccgga agccatcgat gaacgtatac
    18361 tgaaatacaa aatcgatatc gacggcccgc tggcccaggt atgggccgac tataatctgt
    18421 atgtcggcac caaatttata cactgcggca tcgatgtttt ccatttatat aaaagcaacg
    18481 aaggctggaa gatttttgaa ctggccgata cccggcgcaa aaccggttgc caacccgatc
    18541 ccctggatga agttgcggcc tttctcgata cctggcatgc cgcagctgcc aatgcggacg
    18601 ctgaaaaata ctttggagcc atgacgccgg acggggtctt cctgggcacc gacgccaccg
    18661 aacgttggac ccgcgaagaa ttccggatct gggccaaacc tcagtttgat tccaaaaagg
    18721 gttggaactt caaggccacc aagaggaatg ccgctttcct cgaaaacaat accatcgcct
    18781 ggtttgatga ggatctcagc acctggatgg ggccctgccg gggctcaggc gtcctggtca
    18841 aaaccagtga aggatggaag atcaaacaat acaatctgac tatcctggtc ccgaatgaga
    18901 aggtacagga gtatttgaag gtgattgggt tgaggtaggt ttgaggtttg gggtttgagg
    18961 gggtgaggat ttataaaaat cgaatattcc tgaatggtta ctcttcgtat agaacacaaa
    19021 atcgccagta tggcgggatg gagaaaagcg tttgacagcg acccgttaaa caggaagcag
    19081 gcaggcgtga agcattaccg catttttcag ccaaaggaag accaaaactt ggtcatcata
    19141 gagcttgatt ttgatcattt agagcaagcc gaagccatgc gaaaagcgct gaaccaactc
    19201 tttcccaaaa ttgaggggac attagttttt ggtgtccaag taaaaatatt ggacctgatc
    19261 gaatcaactg acctttaaac atcgttgacg atcaacttag gagatctcca tttaccactc
    19321 accgtccacc atccaccttt cagaccacct ctttcttctc cacctttgat tcatccagtt
    19381 cgatcccgaa tccgggcagc gtgggcaagg gcaagatccc attggcggtc aataagggat
    19441 ttttctggaa attcagctta tacggcatat ggttgatcag gtattcccca aaggggcata
    19501 cccccggaga ctggctggct accacgtgaa gcgccgcatg gatattgtgc ccgtgcggaa
    19561 tgacttttgc tccgaaagca gatgcgatat gacaagtctt gaccagttcg ctgaccccgc
    19621 cgcaccattc cgggtcggac tggacgacct ggatggctcc ggcttttaaa aaatgaagcg
    19681 cttcccaacg gctgtagaaa tgttcaccgg ttgccagggg gatgctgctg tgttggctga
    19741 gttgaacaaa actttccaga cggtcggagg ggaaggcttc ctccaggaaa taaggacggt
    19801 atttttccac ctccctgcac caggcattgg caaaaggata gtcccagccc atgtaggcat
    19861 cgaccatgat cagcgcctca ggtcccaggg aacctcttag ttcttccatc aggccaacgc
    19921 tgtggttgta accttgtggc ccgctgcccg ggccataagc catgaaccat ttctggtatt
    19981 taaagccttc gttaaacaac tgcctggctt tggggcccgc tttaccggac tcgacggtaa
    20041 aactcaggca actgccgtat accagcactt ccttgcgggt tggtccgccc agtaaggcgt
    20101 aaacgggggt attgtaatac ttgccacgga tatcccaaag gacattgtcc acggcgctga
    20161 gcgccatcat ataatgtccg gccctgccgt gccggttgtt ccggtacatt ccatcccaga
    20221 gggactcgat agccagggca tttttcccaa gcaagaaggg tttaagttgc tgtaaaacag
    20281 gggttacaca ttcgccgtcg atggctccat agaacccagt gagcccgccg cgggtgcgta
    20341 tttggagata gtactggctg atgtttgagg tggtttccct ggagtccgtc cggtcgctgt
    20401 agaggcccgg tcgccattcg gggtagatat ggatttgttg tacctggtat tgccggttga
    20461 ccccggaaat gctggtatag ggcccggtga tgcggatgac ctcgacggca tcgatgatgg
    20521 tgtcggcggt attatcctca tctgtccatt ttgccaggga gggaaagggg agggcagcca
    20581 ggccggcacc ggctccaagt ccggtgagga attttctgcg gttgaacata aatggaaaat
    20641 acgattaatt cgaattatga aaaatccgac cgccctacaa tttaacgatt cagcaattca
    20701 acgattcaac tcagtacctg gtaagccacc agcgaagcca ggtaggccag cccgcacata
    20761 aaaaagaact ggatcagggg ccacttccat cccccggttt ccctccggac aaccgccata
    20821 gtgctcatgc attgcatggc aaaaacataa aacaacaaca gagatatggc taccggcatg
    20881 gtaaaccggg gttgcagggt ttccgggtct ttttccgcgg ccaggcgctc ggtaatggtt
    20941 gaataatcct cggtactgcc aagactgtat aaagtggaca tggtggcgac gaatacttcc
    21001 cgcgccgcaa aggaagtaaa caaggcaatc cctattttcc agtcaaagcc caggggacgg
    21061 atcatgggct ccatccattt gccgatggtg ccggcatagg aagcttcgag ttttttattt
    21121 gccagcaggg aagcggtttc ctcctcggtc aggttttttt cgatccggat ctgcccaact
    21181 tcttgttcac tggctttcat cttggactga ggcccgtagc tggacaagac ccagaggacc
    21241 atggagatga tcaggatgat ttttcccgcc tccactacaa aagaccaggc tttggacaaa
    21301 gcggtttgaa aggccacttt aaaatccggc actttgtaat ccggcagttc gatcatgagg
    21361 aaacttcggt cctcggattt gatgatttgt ttcaggatcc agccggccag caaggcggtg
    21421 atgattccca ggaggtataa tcccataaac aggaccccct gggtattgaa agggccggtg
    21481 cggtgcgagg aactcaccac aaatcccacc aggatcgtat agaccgggat gcgggcgctg
    21541 cagctgataa aaggggtgac cagggtggtg atcagccttt ctttctggtt gctgatggtg
    21601 cgagcgctca tgatggccgg gatggcacac gccccaccgg ccagcaggga gaccagcgac
    21661 ttgccattga gaccgaattt gagcaggagg cggtcaaaga ggtataccgc cctggccatg
    21721 taaccgaggt cttcgagaac cgtgatgaga aaaaagagga tggcgatctg gggaatgaat
    21781 actatgatcc cccccaggcc ggccagaatg ccgtcagaga tgaaactgct ccatcccggg
    21841 gaggacacga cattttttac ctgggctccc gcccaaccaa agaccgtctc aatccaatcc
    21901 atgggatagg atgcccaggt aaacatggcc tggaaaatga aaaaattgac gattacgaaa
    21961 ataaggatcc cccaccagcg gtgcgtgagg acatcatccg ctttttcgct gaaccggctg
    22021 accttgaagg tgccggccga ggccgacttc ctgaccacgc tggtaaagaa atcatagcgc
    22081 tgcatggtct cccggatctg ggaagcaatg gaattgaatc cgtgccgggt attgatctgc
    22141 ccgatctgtt cggactggcc gggtttaagg tagttcaact gctggtgata gtgggcgacc
    22201 agcaggcttt gatacgcatt gttggtccgc accacgtcat tgagttcatt gaccagggcc
    22261 tgttcctggt aggacagctt atattgctgt tggctcaaag gggacggctt ggccagttga
    22321 cggtccatct cctggagaat ggcgggcatg ctttcctggt tgcgggcatt gatggcgaat
    22381 atgggaatgt cccaggtcgc cttgagcacg ttgaggtcaa tggaaaaccg gtctttgtcg
    22441 gccaggtctt tcatggtcag caccatgatc acgggaagac cgagatcgat gagttgggtg
    22501 aacagcagca attgtttttc caggtgcatg atatcggcca cataaaggac caggtcggga
    22561 aagtctgcgt cgtcgggatg ggtcaggatg ttggtgacga taaactcatc cttggaattg
    22621 gggtggaggc tgtaaacccc cgggaaatcg atcacatcga cccattgacc tgaaggaagg
    22681 cgtaccgttc cctttttttt ctctaccgtg acgccgggaa agtttcccac tttggccgcc
    22741 aacccggtga ggcggttgaa taccgatgat ttgccgctgt tgggattacc caggatcgca
    22801 atttttttca tgatgaaatt tgcgcctccg tttcggttac ctggatcaca tcgagctctt
    22861 ctttacggag gccaaagcgc aatccgtcaa ttttcacata ataggtttgg ccgaaagggg
    22921 attcccttac taaggtggcc aggcttccaa tgatgatgcc tttggatatc aataattttg
    22981 atatcgattc atcggaaaag ccgacgactt tgccatattt gatctccctg aggtttttgg
    23041 ccaggatggg tttgaacatt atttagattt tgtctaaaca aagatacttt cttcatttta
    23101 atgctaaaaa tatatcaggt tcaaaatatt ccagcccggc tcgggataat aaaaaatgaa
    23161 tctaaataat gaaaatcctc atggtttgcc ttggaaacat ttgccgttcc cccatggctg
    23221 aagggataat gaaaaaaatg ctggaggagc gggcgaacgg gccctgggaa gtggattcgg
    23281 ccgctgttgg ggattggcat agaggccagt tgccggacag ccgggcgata aagaccgccg
    23341 cacgttatga tattgatatc acggaccaaa gagcacgcag atcagcgcgg atgacctgga
    23401 ctattttgat catgtattgg ttatggatga agaaatcctg gaatccatcc tggccctcag
    23461 tgagcttccg gaccaccgga aaaaggtgcg cctgatcctg gatgaggcct atcccgggga
    23521 gggcaggatt gttcccgacc cctactattc gggtcggttt gaagagagtt tccgcttgat
    23581 ccggactgcc tgtgaagcct ttttagccgg ccgcggcatt tgacctttcc gggcagaatt
    23641 ctttgtgaaa aattttcatc aaagaaccct gtccgtgcag gcctttcgtt ttttcatgaa
    23701 agccagttga tttattttga ttcagttgac gatcctggag atggtggtca gcaggaaagc
    23761 cagcatacaa atcagccaaa accaaaagcg gatatcccgg gtcaggttga gcggatatcc
    23821 atttacgacc cattttcgtt ttttcatttc tagcacaagg tcaattcaag cccgggggtg
    23881 ctttgagcag ttttaattgt aaaattcaag aacaggggta tcctgttaag ttcaaaaggc
    23941 atgccatagc aaaaagccat aatatgtggc ttgtggcaag gatttaagcc ccttttttcg
    24001 tataaacgga aaacagaatg tatacgggaa tcaatccagc cgggcttccg ctgcccatct
    24061 caggcaatcc gcgaggcttt gcccgaggtc atcgagttgc tctccccgga ggaccgcggg
    24121 agtgaaaagt tttgaaccca gggcaaggct ggccgctccc gccttgatcc aggactggat
    24181 gttttcttta accggttcga cccccccgct gaccaggatg ctggtccagg gcatggggcc
    24241 cagcaggtcc ttcacgaaac ccggcccgaa atggttgcca gggaacaatt ttacaaattc
    24301 acatcccagt tcctcggcct tgctgatttc cgtgacactc ccgcaacccg ggatgcaaag
    24361 gatttttctt ttattgcaca gggagatcac ctccggctgc aggctgggca ttacaataaa
    24421 gtcggctccc gactgaaggt acatggcggc agtgccggtg tccgtgatgg tgcccgcgcc
    24481 aaaacgcaag gcaggaaagt tatttttaca ataggcggag agtggagaga acacctcgta
    24541 agcgaaagcg gcccggttgg tgaattcaag cacccgcaat ccggtttcat aacaaacctg
    24601 caccagttgt ttggcttttt ccaggtctgc ctggtaaaag aggggcagga gggcggtttg
    24661 gtaaatatcg gcagtgattt cgtgacgatc ggcgggatat cgtttgaaca ttgggtgaag
    24721 gtagaaaatt aggttgaggt tgaggttgag gttaaggttg aggttgaggt tgaggttgag
    24781 gatatagcct gaaaaaccta taacgggtgg aaccttaagc ctgctcctcg aaaccaagcc
    24841 caaatctttt tatcttcgcg cagatgtctg acttcctggt ctccgcccga aaataccggc
    24901 ctcagcgttt tgccgatgtg ctgggacagg aacatgtgac ccggacactt aaaaacgcct
    24961 tgctgcgcaa ccagatggcc catgctttct tgttcaccgg accgagggga gtaggtaaaa
    25021 ccacttgtgc gaggatcctg gccaaggtga tcaattgtga aaatgccctg aaaaaccaag
    25081 atgcggaacc ctgcaatgcc tgtgaatcct gccggaagtt tgatgagcag cttaccttca
    25141 acatcattga aatcgacgcc gcatccaaca atagcgtgga gggtatgcgg aacctgatcg
    25201 aacaggtccg ttatcttccg cagcagggca aatacaaggt gtttatcatc gatgaagtgc
    25261 atatgctgag caatgccgcc ttcaacgcct ttctcaaaac cctggaggaa cctcccgccc
    25321 atgccatttt tatcctggcc accaccgaaa gacataaaat catcccgact attctttccc
    25381 gctgccaggt ctatgatttc cgcaggatcc agcctgaaga gatcgtaaaa caactggaag
    25441 aagtagccaa agaccaggaa atcaaaaccg aagccgaagc ccttcatatt attgcccaca
    25501 aggcagaggg ttcgatgcgg gacgcccttt ccatctttga ccggatcgcc agctttggag
    25561 gcaaacaagt cacctacgca ggtacgatca gcaatctgaa cgtcctggac catgaatatt
    25621 ttttcaatct ggtttatgga atgctgcggg aagacagcat gggcatactt aagaccttca
    25681 gggatattca ccaactgggt ttcgaaggga atcatttcat ggatggcctg gcagaccatc
    25741 tccgcaacct cctggtgagc aaagatcccg gagctgccca actgatcgaa ggcagccagg
    25801 acctgaaggc caaataccat gcccaggccc agggcgcacc ggtttctttt atcctcgatg
    25861 cgcttgccat cgtcaacgaa tgcgaccttc aatacgagcg ggccaggaat aaacgattgc
    25921 atgtggaatt ggccctgatc cggatgtgtt atctccaacg tctttccgac gcgatcccaa
    25981 gctttcccca ggaggctgaa aaaaaaaagc ctgaacccgg gccagcgcct ccccaggtga
    26041 tcatcagtcc gccacctccg gttcacctgg ccgtttttga tcaggatccg gttgaattcc
    26101 aaaggcctgc ggcccccgcc gaacaaaagt tgccgaatca ctacgacacg cccaaaatcc
    26161 gctccctgaa agacattcag aaaaaagcgg cgcagagcat ccaggccaat ccggattcgg
    26221 cggtggagct gaagatagaa acctttgaac aagcctggaa ggagtttgtg gacaccgtcg
    26281 aggtcttaag tcttaaaacc gtgctcagcc aggtggaatt ggcgctggaa cagacgaaac
    26341 tgctgatcaa ggtgggttct gcccatgccc ggaatattat ccagctggaa agctctttat
    26401 tggaaaaact gcggaattcc gtgtccgaca aaagactgga aatcgcgatc gtgatcgacc
    26461 ccgaaaaagc caaactcatg gagagccagg tgaaaaagcc ggtttcacta aaagagaaat
    26521 atgaactttt ggtacaagcc aatccaaaag tagaagattt gatcctggct ttaaaactcc
    26581 ggatcgacca tggctgatct ccgtacccaa tccttttgtt tatgtggaaa gttctgatca
    26641 ggccggtttt gtttttactg gacgcggaga cggcccatca tttcactctt gggctgttca
    26701 aatggatttg ccgcatcccg ggctgcagaa gactgattaa agtattattt gctttgcctg
    26761 aacagcaagc cgtggaagtg tttggattaa agtttaaaaa cccggtggga ctcgcggccg
    26821 gcctggacaa agacggtaaa tacctccatg aactttacct gttgggattt tcccatatcg
    26881 agatcggtac cgtgaccccg aggcctcaaa ctggaaatcc aaggccccgg ctgttccgcc
    26941 tccccaaaga ccaggcttta atcaaccgga tgggttttaa caatctcgga gcggatgcca
    27001 tggcacaaag gttgagatcg cggaataaac cgccagacct tatcctcggc atcaacatcg
    27061 gcaaaaataa agacaccccc ctggaacagg ccactgcaga ctacctggcc tgctttgaaa
    27121 aattgcatcc ctatgcggat tattttacgg tgaatatcag ttcacccaat actcccggtt
    27181 tgagaggcct tcaggataaa gccccactga atgaattgct gggggcgatc atgaaagcca
    27241 ataaaaattt ggcccagccc aaacccgttc tggtcaaact ggccccggac cttgacgatg
    27301 cccaacttgc cgaagcaaag gagatcatat tgtcccatgg catcgacggg atcatcatgg
    27361 gcaataccac cctggaccgt tccggattga ggacggaacc ggagcggatc gaaaagatcg
    27421 gggccggcgg actgagcggc ctgcccctct tgaaaaaatc caatgaaagg ctctccgccc
    27481 tgaccggctt acccggtaaa atcccggtga taggagttgg cggcatcatg aatggagcgg
    27541 atgggcgatc taagctccgg gaaggggcgg tgctggtcca ggtgtacagc ggatttatat
    27601 atgaagggcc cggcctggtc cgtcaaattt gtgcagccct taaccgccag accgacgccc
    27661 cgggttaatt ttcaattaca agtgggcaag acagccctat tgttttcgtg aggatagggg
    27721 taaattgcag gatgaataaa aaagggtacc gcagcaacgc cggggagggc ggcgcaatcc
    27781 ctaaaggcgg caacggccac tacagcattg ccctcatcgc ccatgatggt aaaaagctgg
    27841 atatggcctc cttcatcttc cgccaccacg ccatgttgaa aaacatgatc ctttacgcta
    27901 cccacaccac cggtacctat attgaaaagg aaggcattcc ggtcattaaa ttattgtccg
    27961 gccccagggg tggagatgca cagatcgcgg ccatggtcgc cgaaggaaag ctgcacgccg
    28021 tgattttttt ccgggaccct ctggacgccc atccgcatga accggatgtg cagatgctga
    28081 tgcgggtctg cgatgtgcat aatgtcccgc tggcgaccaa tcccgccacg gctacgatga
    28141 ttatagatac cctggctgtg aagcagcctt agcccacaag ttcccgccac gttttttgct
    28201 cacgatggca caaagactta agattctttt ctcccacaaa ggccgcgaag gccacaaagg
    28261 atcaaaggat tagcaaatag aatttttctc ccttcgccgt ttacctcccg cggctgcgga
    28321 actccttctc ccacaaagcc cgcgaaggcc acaaagcatc aaagaattag cgaaaaggat
    28381 tttgctccct tcgcctcccg cggttgcggg acacctttat ccattcctcc atttctccag
    28441 tctcccttcc tcccacaaag cccacggagc ccacaaagga ttagcgaaaa gaattttgct
    28501 cccttagtcg tttacccttc gcccttcacc tttatccatt cctccatttc ccctgtctcc
    28561 cttcctcccg tctccctttc tcccacaaag tccacagagc ccacaaagaa ttagcgaaaa
    28621 gaatttcgct tccttcgcct ttttcccttc gcccttcgcc tcccgctcca gaacgactcg
    28681 ggatgcggga ctcctttctc cccaaagccc gcgaaggcca caaagaatta gcgaaaagga
    28741 tttttctccc ttcgccgttt acctttcgcc tttcaccttt ttacattcct cccttcctcc
    28801 tgtttccttt cctccaactt actaacttac caacttgcta acttgctaac taataaactg
    28861 cccacgatcc cttatttttg cctcctacaa ttactacagt tatgtctact ccttttgatc
    28921 ttgaaatgat ccgtgcggtt tatgcaagct ttggggacaa agtgaaaaat atccggcagc
    28981 aactgggtcg ccccctgacc ctgactgaaa aaatcctttt cacccacctc catgaggatt
    29041 cccccctgaa ggattacgag cggggtgagg attatgtctt ttttgccccg gaccgggtgg
    29101 ccatgcagga tgccactgct cagatggccc tgcttcagtt tatgcaatgc ggcagggaca
    29161 agaccgcagt gcctaccacc gtacattgtg accatttgat ccaggccaaa tccggagcgg
    29221 acaccgattt gaaaaccgcc ctggataaaa attccgaagt atttgccttc ttaagttcca
    29281 tctccgaccg gtacggcatc ggattctgga aaccgggcgc ggggattatc caccagatcg
    29341 tattggaaaa ctatgccttc cccggaggca tgatgatcgg gaccgactcc cataccccca
    29401 atgcgggtgg cctgggcatg atcgcgatcg gagtaggcgg cgccgacgcc gtggatgtca
    29461 tgagcgggat gccctgggaa ctgaaaatgc caaaactgat cggggtaaaa ctgactggca
    29521 aactgagtgg ctgggcttcc cccaaggacg tcattcttaa agttgcagga atattgaccg
    29581 ttgaaggggg caccggcgca gtggttgaat attttggccc gggcgcccag tccatgtcct
    29641 gtacgggcaa aggcacgatc tgcaatatgg gcgctgaaat cggcgccacc acctccacct
    29701 ttggatacga tgcctccatg ggccgttacc tccgggcgac gggtagggcg gtgatcgccg
    29761 accttgccga cggcatagcc caggaactga ccggtgacga ggcctgttat aaaaacccag
    29821 aggaatactt tgaccgcgtc attgagattg acctgagcaa actggaaccg cacgtgaacg
    29881 ggccctatac tccggacctg gcctggccgc tttcggaatt tgcggctgcc gtaaaagaaa
    29941 aggggtatcc cgctaaactc gaagtcgggc tcatcggctc ctgcaccaac tcctcttatg
    30001 aagacctgga ccgcgcggct tttgtagcca aacaggccaa agagaaaaaa ttaaaggtaa
    30061 aatcggaatt cactattact cccggctcgg aacaaatcag gtataccgtc gaaagggacg
    30121 gccagctcca ggcttttgag gacatcggtg ggatcgtact cgccaatgcc tgcggcccct
    30181 gcatcggcca atgggccagg catacggatg atcccaaccg gaaaaactcc atcctcacct
    30241 ccttcaaccg caacttttcc aaacgcaacg acggcaatgc caatacccat gcttttgtgg
    30301 cttcgcctga aatcgttacg gctctcgcga ttgcaggcac cctcactttc aatccgctca
    30361 ccgatacgct gatcaacgaa gacggccagt ccgtgaagct cgatccgccg cagggcgagg
    30421 aacttccctc caaaggattt gatgtggaag atgctggcta ccaggcccct tccgcagacg
    30481 ggtcctccgt aagggtcgaa gtgagggagg acagcgaccg gcttcaattg ctcacccctt
    30541 ttaaacccat tacccaggaa caagtcaaag gaatgcggat tctgatcaaa gcaaaaggta
    30601 aatgcaccac cgaccatatt tccatggccg gcccctggct caaataccgc ggccatctcg
    30661 aaaacatatc caacaattgc ctcatcggcg ccataaatga tttcaacggg gaagcgaata
    30721 aagtgctcaa cctggtgacc aatgagtaca tggccgtacc cgattcggcg aggacctacc
    30781 aaaaggccgg cgtcggaacg gtggtctttg gtgaattcaa ttacggggaa ggctcttccc
    30841 gcgaacacgc ggccatggaa ccccggtacc tgggagtcaa agcggtgatc gtaaaatctt
    30901 ttgcccgcat ccaccagacc aaccttaaaa aacaaggaat gctggcactg accttcacca
    30961 atcccgatga ttacgacctg atccgtcagg atgatacggt ggatatcgga ggcttcgaag
    31021 attttgcacc cgatgaagcg ctttatgtcg tcctccatca tgcggacggc acgatggacc
    31081 ggtttgaagt ggatcacaat tacaacgaac aacaaataga atgggtaaag gaaggcagcg
    31141 ccttaaataa gatccgtaag gaccttgggg tttcctgatc gaaggcccaa tctccaaata
    31201 aaaatttgaa ccgcatagct cgtcttgaat gctaaggaac cgtaggggaa aggaggagtt
    31261 ctttctttgc ggattttcat tgcggtctca ggggccttgg cgattagaaa aggattataa
    31321 accgcaatga gcgcaaagag cgcgaagatt ttgcagagaa atgagaaggg catgcaggat
    31381 tagcggcgga tttgattaat ccaacccgga tccctcccgg ttttgtactt tgagaggaat
    31441 ttattgcttt caggtatgga aacctcctac gatgccatag tgatcgggtc cggcatttcc
    31501 ggcggctggg cggcaaaaga actctgtgaa aaaggcttaa aaaccctggt gcttgaacgg
    31561 ggaagggata tcaaacacat caaagactat cccacggcga ccatggcccc ctgggatttt
    31621 caataccggg gcagggtgag ccgggagtac atcgaaaaaa accccctgat cagcaaagcg
    31681 gccggtttcg gtgacgatac caaacatttt tttattcagg atgcggacca tccctatatc
    31741 caggaaaaac cctttgactg gatccggggc taccaggtcg gaggaaaatc gctcatctgg
    31801 ggccgcgcat gccagcgctg gagcccatat gagtttacag cccccgaacg ccagggctat
    31861 gggatgaact ggcccataaa ttaccaggac atagccccct ggtacagcca tgtggaaaaa
    31921 ttcgttggag tatgcggcag ccgggacggc ctcgaagcga tgccggacgg ggagttttta
    31981 cctccttttg gcctgaattg cgtagaaacc catatgcggg ataaaatcaa agccacttat
    32041 ccggaccggc atatggtcga cgcccgctgg gcgcagatca ccagggcgga tgaaatctat
    32101 cttcagcagg ggcgcggaaa atgccaggct aggaatatgt gcatgcgggg ttgtcccttt
    32161 ggcggctact tcagttcggt ttcgtcaacc ctgccctggg cggagaaaac gggtaatctg
    32221 accctcaggc cccattccgt cgtgcactcc atcctgtata atgaaggcac aaaaaaggca
    32281 agcggagtca gggtcatcga cgcggtcagc aaggaggaaa tgacctttca tgcgaaagtg
    32341 attttcctca acgcctccgc cttgaattcc ctgctgatcc tgctccattc caaatcctcc
    32401 cggttccccg aaggcctggg caatgaatac gaccttcttg gccgctatgt tgcttttcac
    32461 aactaccggt cttatacttc gggtcaactc gacggttttg aagataaata ttattatggc
    32521 cgcaacccga ccgaacccat catagcgaac taccggaacc tgggaaaaca aaccatggat
    32581 tttgcgggag gctacaccat tttcaccgga gcttaccggt cgggatttga aacttatgag
    32641 ggcccccaat tcggcccgga atttaaggag catatgaccc ggcccggacc ctggagggtg
    32701 tacatgtata tgcagggaga aaccataccc aaagaaacca accgggtgag cctgagcgac
    32761 tcggaaaagg atccctgggg cattcccctc ctggtgaccg atgtgggtta tgatgacaac
    32821 gatgaccgga tggtgaaaga ctttatgcag gaatccgtgg atatgatgca agccgccggc
    32881 tgtcgcgaca tcagcaccgg tgacaatcac caggctccag gcctggatat ccatgagatg
    32941 ggtggctgca ggatgggcac aaaccccaaa aactccatgc tcaacgcctg gaaccaaatg
    33001 catctctgta aaaatgtatt cgtcaccgac ggcgcctgca tgaccagtac gggcaatcaa
    33061 agcccatcca tactatatat ggccctcacc gcccgcgcag tgagtcacgc cgtggatgag
    33121 ttaaacaaac gcaatttgta gtcacggacc ctttctcctc ccacggttgc gggaccccct
    33181 tttcccacaa agcccaccag gcccacaaag caaaccaagg atttgcaatt agaattttgc
    33241 tcccttcgcc ttttaccttt cgcctttcac ctttttccat ttcgcctccc gctattgcgg
    33301 gactcctttt tcccacaaag cccaccaggc ccacaaagca aagcaaggat ttgcaattag
    33361 aattttgctc ccttcgcctt ttacctttcg cccttcgcct ttttcccaca aagcccacca
    33421 ggcccacaaa ccaaacaaag gattagcaaa aagatttttg ctcccttcgc ctttttccca
    33481 caaagcccgc gaaggccaca aagcaaacaa aggattagca aaaagaattt tgctcccttc
    33541 gccgttcacc tttcgccctt cgcctctttc ccttcgccgt ttacctttcg cccttcgcct
    33601 ttatcccaca aagcccacca ggcccacaaa gcaaacaaag gattagcaaa aagatttttg
    33661 ctcccttcgc ctttttccct tcacccttcg cctttcgcta ttgctggact cctttatccc
    33721 acaaagcccg cgaaggccac aaagcaaagc aaggatttgc aattagaatt ttgctccctt
    33781 cgccgtttac ctttcgccct tcgccttttt cccacaaagc ccaccaggcc cacaaagcaa
    33841 acaaagtatt tgcaaaaagc attttgctcc cttcgccgtt cacctttcgc ccttcgcctc
    33901 tttcccttcg ccgtttacct ttcgcctttc acctatcacc tttttccatt tctcctatct
    33961 cccttcctcc cgtctccctt tttcccacaa agcccgcgaa ggccacaaag gaaaccaagg
    34021 atttgcaaaa agcattttgc tccttttgcc gtttaccttt cgcccttcgc cttattccct
    34081 tcgccgttta cccttcgccc ttcgcctttt tcctttcgtc tcccgcggtt gcgggactcc
    34141 tttttcccac aaagcccgcg aaggccacaa agcaaacaaa ggattagcaa aaagattttt
    34201 gctcccttcg ccgtttacct ttcgcccttc gcctttcgct attacgggac tcctttttcc
    34261 cacaaagccc gcgaaggcca caaagcaaag caaggatttg caattagaat tttgctccct
    34321 tcgccgttta cctttcgcct ttcacctttt tccatttctc ctgtctccct tcctcccgtc
    34381 tccttttctc ccacaaagcc caccaggccc acaaagcaaa gcaaggattt gcgattagaa
    34441 ttttgctccc ttcgcctttt acccttcgcc cttcgccttt tacttttcgc ctcccgtagt
    34501 tgcgggactc ccttcctctt gtctcctaaa atcccttatc ttgaaaaaaa attctttctc
    34561 atggatcagc gaatcatcaa cctgtacgac gaatacattc acaagcccct gaaccggcag
    34621 gactttctca accggttgac caccctggtg ggcagttctg cggcagccat ggccattctt
    34681 ccccagattg aagtggattt tacccatgcc aaccgcacgc cggatgccga tttatttacg
    34741 gaatacatta cctatgccgg agtccagggt accatgaggt gttatgtggc gcggcccaag
    34801 gaagaaaaaa agtaccccac ggtggtgatc atccatgaaa accgcgggct caatgcacat
    34861 atcgaagatg tggcccgacg ggcggcccag gccggttatt tggctatcgc gccggatgcg
    34921 ctctctcccc tgggactgac ttttgcaaac gaggatgagg ccaggaccaa gttccgggat
    34981 ttgaaagcag aagacaacaa tgccaacttc atccgggtct tcgaatacct caagacccga
    35041 aaagacagca acggcaatta tggttgtgtg ggcttctgct ggggaggggc catggccaat
    35101 acccttgcgg tgaacgtacc ggaattgaaa gccgccgtgg ccttctatgg ccgtcagcaa
    35161 cctgcggaaa acgtgcccaa aatcaaagcc gccctccagt tgcattatgc cgaaatggat
    35221 gaaggggtca acgccgggat cgtcgcctat gaggccgcac tgaaagccaa taatatcccc
    35281 tatgaacttt atatgtatgc aggcgcccag catgctttcc acaacgacac ggccccctcg
    35341 cgttaccacg aagcatccgc caaactcgcc tggcaaagaa ccctcgattt ttgggcgaaa
    35401 tatttataat aattcccgag gaagatccgg attggcacgg gacttgtatt ttgaaatcac
    35461 aaaaccaagc tattttttga cccggattgc gataaaaatt ttaattttgc agccatgtta
    35521 attcatcacg gacatcatca tcaccatctt ttgcagcatg caaacgggta atggtttgtc
    35581 ctgattgaaa acataaaata taaatcaaag gcttgccgga cccggtgagc cttttttatt
    35641 ttaaccaaat gggtatatga ccagactaaa tatagcgatt caaaaatcag gcaggatgca
    35701 cgacgattgt atggtgctcc tgcgggactg cgggctcagg attgaaacgg tctcggacca
    35761 gcttaaaacc agcgcggggg gattccctct ggatattctt tacctgcgca acggcgacat
    35821 tcccgaatac cttcaggacg gggtggccga cattgccatc ctcggtgaaa atacggtgat
    35881 tgaaaaaggc agccgggtga ccggcctgct tcccctcgga ttttccaaat gccgcctgtc
    35941 tatcgcggtc cctcaaaaaa gcggatacga agacctccgt tttttcgagg gcaaaagaat
    36001 cgccacctcc tatccccgca ccctggcaaa atatctccgg gataaagggg tcaccgcgga
    36061 aatccaccag atctccggca gtgtggaaat cgctccccat atcggcctgg ctgatggaat
    36121 ttgcgacctg gtttcctccg gcaatacctt atttcagaac aagctggaag aaaaagaagt
    36181 catcctgcat tcgcaagcct gggtctatgc caacaatgag ctggatgcag acaaaaaagc
    36241 cctggtggat cagttgcttt tccggatcaa agccaccctc gaagcgcgta aaaacaaata
    36301 catcctgctg aacgccccta aggcttcgct cgataaaatc atctccatcc tgccgggtat
    36361 caaaagccca accatcctgc ccctggccat tgaaggctgg tgctcggtac attccgtaat
    36421 ccccgaaaaa gaattctggg aagtcatcga tgccctcaaa caggcggggg ccgaaggcat
    36481 cctggtgatc ccgatcgaaa aaatggtgag ctgaatatga aaatatacct tgaacctccc
    36541 agggtgcaat gggccgaaat cacccgtcgc ccatccggcc aggacctcag ccaggaatcc
    36601 gtcgtgcaat ccatccttga ccgcgttcgg caggaaggcg atacggccct caaaaagtat
    36661 acggaaaaat tcgatcaggt cagcctccag tccttcgggg tcagccagga ggaaatggac
    36721 caggccgaaa aaaaactgga ccaaagactg aaggaagcca tacagattgc caaatccaat
    36781 atcgaagcct ttcaccgttc ccaatggcat gaacccggga tcgtggaaac catgaccggg
    36841 gtgaaatgct ggcaaaaaac cgtccccatt gaaaaagtag gtttatacat cccgggtggt
    36901 acagcccccc tgttttccac cgtcctgatg ctggcaacac cggccgggat agcgggctgt
    36961 agggaaatct tgttgtgcag cccaccggac aaattcggtt ccgtacatcc ggccatcttg
    37021 tattcggcca ggctttgcgg ggtgacccgc atcttcaagg ttgggggcgc ccaggccatc
    37081 gcagccatgg cttacggcac gaaagaaatc cccgcggtac acaaaatatt cgggcctgga
    37141 aattcttttg tgaccctggc aaaaatgaag gtgcagcagg aggggattgc cattgacctg
    37201 cccgccggtc cttccgaagt cctggtgctg gcggatgagt ccgccgaccc ctcttgggtg
    37261 gccagtgatt tgttgtcgca ggcggaacac ggcctggaca gccaggtggt gctggtagcg
    37321 gattcggaag attttgtccg ccaggttggc ctggaaatgg aaagccagct ccaggaactt
    37381 cccaggaaaa aatacgccac cggctccctg gcgcatagtc acgccctggt cctgaaagac
    37441 cggaaagatc aactggattt tataaatgaa tatgcccctg aacacctgat cctcaaccac
    37501 agggacgcgg acgccatctc caatgaagtg atcaatgccg gatcggtttt catcgggccc
    37561 tattccccgg aatcggtagg agactatgct tctggcacca atcacacgct tccgaccagc
    37621 gcctatgcca aagcatacag cggggtcaac ctcgacgcat tcaccaaaaa aataaccttt
    37681 cagaaacttg accccgaagg ccttcaggcc ataggccctt atgtggagct catggcccag
    37741 gccgaacaac ttaccgccca tcaacgggcc gtatccaaac gattaaacaa actgaaccgt
    37801 ccatgaccat cgaatccctc atccggccca atattttaaa actgaagccc tatgcctcgg
    37861 ccaggcatga attcaccggc aagggagagg tgttcctgga cgccaacgag aatccctttg
    37921 ataccggggt caaccgttat cccgatcctt tgcaggaaaa gcttaaatcg gccatcggcc
    37981 ggctaaaaaa catccctacg gaccaaattt tccttggcaa tgggagcgat gaggccatcg
    38041 acctgatctt gcgcattttt tgtgtgcccg gccgggacga aatcatggtc ctgccgccta
    38101 cctatggcat gtaccaggtg agcgccgaca tcgccgatgt ccaggttaaa aaggtcctgc
    38161 ttcaagccga tttccagccg gacctcccat ccataaggaa taacctcggg ccccatgcca
    38221 aggtgatctt tatttgcagc ccaaacaatc ccaccggcaa cctgatccgc cgggaggcca
    38281 tcgaagagat ccttgaattt ttcgggggaa tcgtggtggt ggatgaggcc tatacggatt
    38341 tttgccctga ggccacgctg ctgcctttat tggaaaaata cgaaaacctg atcgtgatgc
    38401 agaccctgtc caaggcttgg ggcatggccg cgatccgcct gggaatggct tttgcctccc
    38461 ccctgctgat ttcttatttc aataaagtga aggcgcctta caatatcaat atcctgaccc
    38521 agcgctttgc cctggaacgg ctttcgaaag aaggcgaaat gaaggcccag gtgcaatcca
    38581 tccggcagca aaaaacatgg ctgacccgcg aactggaatc cctgaacgag gtgctgcagg
    38641 tctatccttc ggacagtaat tttttattgg tcaaagtcag gcatgcggag gccctttacg
    38701 gttatttgtc ggcccagggg gtcgtggtgc gcaaccggtc cagggaaccc ctctgcgaaa
    38761 attgcctccg gatcaccatt ggcacggaaa cggaaaacag gactttactg gaaaaaatga
    38821 atttgtttca aaaccaagcc ccggtatgaa tatgcagaaa gttttgttta tcgatcgcga
    38881 cggcaccctg atcgtggagc cccaggacaa ttttcagatc gattcgctgg aaaaactgga
    38941 gtttataccc ggggtcttca cctggttggg taaaattgcc agccagttgg attataccct
    39001 ggtcatggtg accaaccagg atggattggg gactccttct ttgcccgaaa cagatttctg
    39061 gccggcccag aataaaatga tgaccgcctt taaaaatgaa gggatccatt tcgctgccgt
    39121 gcacatcgac cgctcctttg aacatgaaca taaggaaacc cgtaaacccg ggaccggtat
    39181 gcttaaagaa tacctgaccg gttcctacaa cctcccggaa agttttgtga ttggcgaccg
    39241 gctcacggat gtgcaactgg ccgctaattt gggttgccag gctattctat ttaacgacga
    39301 tttggactcg gcggcgcttg cggtggcaca tcagagccgt tcctggaaat cggtttatga
    39361 attcctccgc ctccgttcca gggtcgtcca ccatcaaagg cataccaggg aaacacagat
    39421 cgatatccgg ctggacctcg acggcaccgg taaaagcagc atatccaccg gccttggctt
    39481 ttttgaccat atgctcgaac agatcgcccg gcatggggaa tgcgacatgg aagtccgctg
    39541 caaaggggac ctgcacatcg acgaacatca cagcattgag gatacgggca tcgcgcttgg
    39601 ggaagctttt cttaaagccc tgggggataa aaaaggcatg gaacggtacg ctttccattt
    39661 acccatggat gaagcggatg ccgaagtgct gctcgatttt ggaggcagag cctggctggt
    39721 atgggaagcc gaattcaaaa gggaaaaaat cggggaaatg ccgaccgaga tgttttacca
    39781 tttttttaaa tccttctgcg acgcggctaa atgcaatctg caggttcggg tccggggtga
    39841 aaacgaacat cacaagatcg aagccgtctt caaggccttt gccaaatgca tccgccaggc
    39901 aaaacggcgt gacccggcat cggacctcct gccttccact aaaggggttt tataaggaaa
    39961 gccaaaaaac atgaagcaga aaattgcagt gatcgattac aatgcaggca atgtgcagtc
    40021 ggtgctcttt gccctcgaac ggtttgacgt ggcggcggtg cttacccgtg acccggacga
    40081 gatccgaaaa gcggacaaag tactcttccc aggggtggga gaggcatcca cgacgatggg
    40141 ctttctccgt gcccggggat ttgaccaatt gatccccggc ctgacccagc ccgttctggg
    40201 catctgcctg ggcctccagt tgctctgcga acattcggaa gaaaacgata cggcctgcct
    40261 gggcattctt cccgtaaagg tcaaacggtt tgtggctacc tctcacgaat tcaaaatccc
    40321 gcacatggga tggaaccagc tggagcttaa ccagcaggat tggctggccc cttccatgga
    40381 ccggcagtac gtgtattttg tccacagtta ttatgcggag ctcaatccct atacggtagc
    40441 ttcctgcacc tattgccagc ccttcagtgc cgtgattcac aaggacaatt tttatgctac
    40501 ccagttccac gtggaaaaat cagggccggt ggggcttaag atcctggaaa cgtttttgga
    40561 cctgtaaacg ctaagtcaaa tatgatcgtc tttcccgcaa tcgacctcat cggaggaaaa
    40621 tgtgtccgac tcaccgaagg ggattttgaa cgccttacgg attaccaggc ggacccggtg
    40681 gatacggcaa aaaaatacga ggatgcaggc ctcagccacc tgcatctcgt ggacctcgac
    40741 ggtgccagga acaaaaaagt gacccagtat aagatccttg aaaaaatctg cagcaagacc
    40801 aatctcaaag ttgattttgg cgggggtata aagacggacg atgacctgcg catcgtattt
    40861 gaaagcggcg gggcacaggc caatgtgggc agcctggccg tctccgagcc tgaaattttc
    40921 gaagcctgga tggtcgaata tggctctgac cgcatcatcc tggctgcgga tgtccgggat
    40981 atgaaggtgg cggcccatgc ctggaaggaa accagcccaa tctcgatttt cgacctgctc
    41041 gaccgctttg aacctcgcgg actgatatac cttacctgca ccgatatttc ccgggatgga
    41101 aaactcaccg gggtaaacca tgaattgtac aagaccctga tggaaaaata tcctgccctc
    41161 cgggtgaccg ccagtggcgg agtgcatcat gtgaaggatc tgcagggact gttggagatg
    41221 caatgttttg gcgtgattgt cgggaaggcc atttacgaaa acaaaatccg gctggaggaa
    41281 ttggtttcct tccagttgaa ctaaaaaaca tttcatgtta gcgaaaagga tcatagcctg
    41341 cctggacatc aaggatggcc gcacggtgaa aggcatcaat tttctcgggc tccgggatgc
    41401 cggtgatccc gtggaactgg gcaggctgta tagtgatcag ggcatcgacg agttggtgtt
    41461 tttggatatc accgccaccc atgaaaaaag gaaaaccttg agcgccctgg cccgggcggt
    41521 agccaaaaac ataaacatcc ctttcaccat cgggggagga gtaacttccg tggaggatgc
    41581 ctccgccctg ctggaatccg gggccgataa agtctcgatc aattcggctg cggtgaggga
    41641 acccgatttg atcaggcgac tcgcccttga atttggaagc cagtttgtgg tcctggcggt
    41701 ggacggaagg ctgaccgaaa acggttggga agtttatata caagggggcc ggttatcaac
    41761 aggcctgccc atgtacgatt ggatcaggaa gggggttgac ctgggagccg gggaaatcct
    41821 gtttacttct atgaatcacg acggaatgaa atccggcttt gcggtggaag ccacccgtat
    41881 ggtttccgaa tcggtatcgg tgccggtgat tgccagcgga ggagcgggca ccatggagca
    41941 tttcgccgat gtcttccggg agggcaaggc cgatgccgcc ctggccgcca gcatttttca
    42001 ttaccgggaa atcgggatac ccgaattgaa agactttctg cgtaccagaa atatagctgt
    42061 cagaaatcag ccatcagaag tcagcctata gccatcagct atcagcccat agctatcagc
    42121 tgaaagctga cttctgaaag ctgacatctg aaagctgatt gctctttctt atctttcccg
    42181 gataaaactc tcgaaccatt gaacaagggt atagcgaaaa tcataggggc ctcttccgta
    42241 ggcacgctca tcgaatggta tgatttttac atcttcggca gcctggccgt gttgattggt
    42301 aaaaaattgt ttccgccgga tgcaggcgcc tccgccctga tcaatacctt ggcgattttc
    42361 gccgcgggct ttattgtgag gccttttgga gcccttttct ttggccgcat cggggacctg
    42421 gtggggcgca aatacacttt tatgctcacc ctggtcctga tgggggtttc caccttcctg
    42481 atcgggctga tcccgggatt cgattcctgg ggatatgccg ccccgctggc cgtgctggtc
    42541 cttcgcttgc tccagggatt ggccctgggc ggggaatatg gaggtgccgc gacttatgtt
    42601 gccgaacatg ccccgccaac gcatcgggga atatacacca gctggattca aacgaccgcc
    42661 actctgggcc tctttttatc gcttggtgtg attgtctata cccgttacct cataggtacc
    42721 gaagcattta atgactgggg ctggcgggta cccttcctgg tctccatctt cctggtcggg
    42781 gtttcggtct atatccgcat tcgtatgcgc gaatcgcctt cctttgcccg gatcaaggcc
    42841 gaaggcaaaa tttccgtcaa tccgatcaag gagagtttca gccacaaggc caattttaaa
    42901 atggtcctgc tcgccctttt cggggcggtc atgggccagg gtgtaatctg gtacaccggt
    42961 cagttctacg cccagtcttt ccttgaaaac acctgcaaga tcgaattcaa ccaaagcagg
    43021 tacattctgt tgatcgcgat cgctttcgcc acccccttct ttattttttg gggatggctg
    43081 agtgataaaa tcggcaggaa atggatcatg atcgccggga tggtgctcgg ggtactgacc
    43141 tatcggccca tttaccgtta tttctacgaa aaaaccaaca gcgtggtcct cgagcggtct
    43201 gaaccggtca aatcgacccc cctgcccctg atccgcatga aaaacgacga cggggattcg
    43261 gtgtttgtca atcagcggat ccaaaccctg gccaacggga tcagtttcaa gcaggcaagg
    43321 gtggatatcc ttttcaggga tccggagaag gcggaccgtg ccctgggaca atttgcgatc
    43381 atcgacaaga ccctgccccc cggtctgttt tggatattcg tatttcttgt ttttatccag
    43441 atcctgtatg taaccatggt ctatggcccg attgccgcct tcctggttga actgtttccg
    43501 acgcggatcc ggtacacttc gatgtccctg ccttaccata tcggcaacgg ggttttcgga
    43561 ggcctggtgc catttattgc tactctgatc agcagtttcc cgggaagcaa ttatctgtcg
    43621 ggcctgtggt acccgattgg ggtggccctg gtctgtgcca tcatcggcag tctttacctg
    43681 gagaacaaga taaacgtgga aaaccgggac taaggctaag gttaaggtta aggctaaggt
    43741 taaggcttaa cctcaacctc aacctcagcc ttaaccttaa cctcagcctt aatctcagcc
    43801 tcaacctcag ccttctccaa acccggtttc gtctttttcc cctaagttta cacttccatt
    43861 aatcgcgtga atatgttact gacatccacc aataccattg aagggcataa gatccggcat
    43921 tacctgggcc tggtgaccgg ggataccatt atcggcgcaa acctggtgaa ggattttttc
    43981 gcctccatca cggatatcgt tggtggccgg tccggggctt acgaaaaggt gctgaaggaa
    44041 gccaagtctt tggcggaggc cgagatggtc aacaaagccc tgtccatggg cgccaatgcg
    44101 gtgatcggga ttgattttga ttacgaagtc ctgggcaggg gcagcatgct gatggtttcc
    44161 atgaccggga ctgccgtgat ttacgatgag tttgcaaaaa aggaagcatg aaaacagacc
    44221 tcagccagat ccggtacgat gcgggcacag ggctcattcc cgccgtgatc caggatgaaa
    44281 tgaccgggaa ggtattgatg cttgggtata tgaaccggga agctttggaa aaaacccggt
    44341 ccacgggcaa ggtgactttt ttcagcagga gcaaacaaag gctttggacc aagggagaga
    44401 ccagcggcca tttcctccat ctcaggtcta tagccaccga ctgtgatgac gatactcttt
    44461 tgatcaaggt ccgtcccgaa ggccctgtct gccatgaagg atttgacacc tgttttcgtg
    44521 aaatcaacct gtccgctgat tttatccagg agcttgaaaa catcatcagg gacaggaaag
    44581 ccaatcccaa agcgggttct tataccaatt ccctttttga ttccggcgtt cagaaaatag
    44641 cccagaaagt gggggaggaa tctgtggaaa ccatcctgga agcccaggct cccggaaaag
    44701 aagcactggt ctacgaagcc agcgacctgg tctatcacct gctagtcctg cttcaggcca
    44761 aagattcgtc ctggggggag gtgatcgatc aactcaaatt aaaacacggt gcccggtgaa
    44821 catccatacc aggcttattt attttccccg ataggatata tctatctggc tcgctctgaa
    44881 atgggactga cccacgttca cctgagccgg aaggagcccg gcgatgattt ttcccaggga
    44941 caggacgacc tgcttttgga agctaaaaaa caactggctt cctatttcag cggatctctt
    45001 cagcgttttg atcttccgct tgatttcggt aaagcgcctg aattcagcca ggcggtgtgg
    45061 cggttgttgc tgcaaatccc ctatggccat acccggtcat acggagccct ggcgcgggac
    45121 ttgggagata tccgcaaatc gagggccgta ggccatgcca atgcggtaaa cccaatggcc
    45181 atcatagtac cctgccaccg ggtgatcggt tctaccggcg ccctgaccgg ttacgcttat
    45241 ggaatggatg ccaaggaatt tttactggct ttggaaaatc caaaccgggg taaacagacc
    45301 gaactgtttt agctttgcaa ttaaataatt aaaataatta atgaattaaa aattaagggc
    45361 agaccattct taattattaa ttccttaatt ttttaattac tactagcttg ccatcttcaa
    45421 ttccctttcc ttcaacccca gggtattgcc ataacggttc acgatcttgc ttttcaggtc
    45481 ctggcgggcg aagaaaatcc ggcttttgac ggtgcccagg ggaagttgca tgtcgtcggc
    45541 gatctcctgg tatttgtacc cttcgtaatg tttcacgaat ggatagcgga tggattcgtc
    45601 cagttcatcc accatatgag taagttcatc catgaggata ttggactcgc cctggtttcc
    45661 tgtggaattg gatcctgaat tcaggaaaaa caggttgtcg gtggaatcaa agaggacccg
    45721 ggtcttggct ttcttcctgt aattgtttat aaacaggttc cgcatgatcg tgagcagcca
    45781 ggctttgagg ttggtccctt cctggaattt ctcccggttg gtgagggcgc gaaaataggt
    45841 ttcctggagc aggtccttcg catcatcctg gttacgggtg aggttgaagg caaagcggag
    45901 taatctatct ttgtgcaggt ccagggcgga aagaaaactg ggctgggtca tcttgtttaa
    45961 tttttatgat tcaaagttaa acaaaaaatc cactgtttag actttttaag aaaaaattaa
    46021 acaaaaaaaa ttactgttgg gcttttctga ccaggcgata ggctataatt gcaaatataa
    46081 tattaggcaa ccagataccc aagacagaag gtatattagg gcttcttgaa aatacgattg
    46141 aaaattttga aataaataca aaaaccgatc caagggcaat gcccagggcg agatgcagac
    46201 ccaaacctcc cctgactttc cgccctgcga tggctacgcc gatgacggta aggatgagga
    46261 tcgtaaacgg atcggccgtt ctgcgctgga tttcattgat ataaaccttg accggattga
    46321 gccccctgga acgttccttg tccaccaggg cctgtagttc tggagtggtc atcaggtctt
    46381 ttttcttgga ataaaactca aagtcagaag gcaggatatt gagggccgta tcgatttgct
    46441 ggtttggatg gagggtgatc gtttctacgg agtcctcgta ggtccggatc tcataatccc
    46501 tgattttcca actgttccgg gtttctttgt tccattccag gaactgggct ttgagcatgt
    46561 aaatcagggt ggtgcccctg aatttttcca gccggaagga ataacccagg ctgtccgcgg
    46621 ccgaataact tctcaggtat actttggtat ccggggagat gaaaaaatgc atatttgttc
    46681 cggtggtaag ccggtcgctg gaccttttga taaacctcct ttcaaattcc agttttggtt
    46741 tattggctat cggaatgata aaatgattgg cgatcaaaag gatgaccgtc agcaaagaag
    46801 ccgccaccag gtaggggcga aggagcctgg ggaaactgac gcctgcgttg agtatggaaa
    46861 tgatttccga attataggcc agccggctgg tgaaaaagat cacggcgatc agcgaaaaaa
    46921 ggggccagag caggccattg atgaaaggga tgtaattgag gtgatactgc cccaggattt
    46981 cccccaggct gagcaccttg gacgcatcca cgaatttttc tgccttttca ctgaaatcaa
    47041 tgaccatggc gatcagggtg aataaaaagg ccgtgaagaa aaaacttccg aggtatttcc
    47101 ggatgatata ccagtccagg attttcaatt caaccgggtt ttgatttttt ccaacatctg
    47161 gtttttccag ggggtaaaga taccttcttc gattttttgc ctcgcggtat ccaccaggta
    47221 gaggtaaaaa ctcaggtttt ggatgctggc gatgcgggcg ccgagcaatt cattgctctg
    47281 gaccaggtgc ctcaggtagg attttgaaaa aggctggcta taggtttccg gcaggccggg
    47341 atcaatgggg ctttggtcaa atttccacct ttggtttttg atgttcagga tgccttccgt
    47401 ggtatagatc agcccgtggc gggcattgcg ggtgggcagc acgcagtcaa aaaaatcgat
    47461 gcccagggcg atggactcca ggatattttc aggggtgccc acgcccatga ggtaacgccc
    47521 tctggatgcg ggcaaatatt gattgaccag gcggatcatt tcgtacatgt cctggttttt
    47581 ttctcccacg gacaaaccgc ctatggcata aaccggggcc tcaaagccgg agatgaactt
    47641 cgccgattcg gtccgcaggt cttgatacac ggaaccctgg caaatgggca gcagggccgg
    47701 ctgataatcg taaggaaggc tcgcctggtt aaaataactg aagcaacgct ccagccagcg
    47761 atgggtcagt cccatcgact gtttggcata ggggtattca caggggtaag gcgggcattc
    47821 atcaaaggcc atgatcaggt cggctccgat ctggagttga atttccatta cggcttccgg
    47881 ggtgaataaa tgccttgacc cgtcgatatg ggaagaaaag gatacccctt cttccctgat
    47941 ctttctcaat ttgctcaggg aatagacctg gtagccgccg ctgtcggtca ggaccggccc
    48001 gggccattgc ataaaacggt gaatgccgcc cgcagcctgc aatacgccgg tgccggggcg
    48061 gagataaaga tgataggtat tgctcaggat gatccgggca tgcacgggtt tttttaattc
    48121 ctcgggactg acccctttga cggaagccgc cgtaccgaca ggcataaaca ccggggtggg
    48181 cacctcgccg tgatccgtgc gcaggatacc gctgcgcgct ccgccggccg ggtcagtggc
    48241 ttttaactca aaggataccc ccataccctc atacaaaatg cctggaactg tccagcctca
    48301 acaacacggc ggtcatcagg gaaaatacga tgcaggaaga gcccccgtaa ctgataaagg
    48361 ggaggggtat gcccatgatg ggaaccaggc ccatggtcat accgatattg ataaaaaagt
    48421 ggacgaggat aaaacccagg aagctgtagg catagacgcg ggtgaaggta ttcctggccc
    48481 tctccccgat ggcgatggcc cttaacatca gcagtacaaa caaaccgatg atggctatga
    48541 tgcccaggaa gccctgttct tccccgatgg tgctgaaaat aaaatcggtg ctctgttccg
    48601 gtacgtaatt caacttggta agggtgccct ccagaaaacc tttgcccgaa aagttgcccg
    48661 aactgatggc cagtttggat tgcagtacgt ggtacaggtt accccgcgga tcgcacattt
    48721 ccggcctgag ccagacgttg atccgttcac gctggtgcgg tttcagaaaa ttatcgaccg
    48781 tatactggga tactttcgtg aaagcggctc ctgcaaggat ggagggaacg aggatgatga
    48841 gcaggcgctg taaacgttgc tggtatccct gataaataaa aaaagcaaga ccggttccga
    48901 gtaagcccaa tactgccatg atcggaactt ttgaccaata agaagccatg atcagtcccc
    48961 agctggccag tactataaac agttgttggt tgtttttccg cagttggctg tagatcaggg
    49021 tggatcctat ggcaaggata taagcgatga cataaagggg atcgaatttg agcgccagga
    49081 taaagagcgc cagcagcgcc agcccgatga tataccacac cgggttaaat ccttcccgga
    49141 acaggaggac aaaaaatgaa ccgaaagtga tggccgaccc cgcatccggc tgcatcaaga
    49201 tcaggccgat ggggaccgca aaaagcatca tggagatcag ccttgacttg aattgtttga
    49261 ggttggtctt gaaataggac aaataggccg ccatggcaag catggtcccg aatttggcga
    49321 actcggaggg ctggaacgaa aagccgccca ggttgaacca ggaggtcgag cctttgattt
    49381 tttgtccaaa aaacaggacc gcgaccagga aaagcagccc cagcaggtag atcgggtagg
    49441 agagcgaact ccaaaactta aaatcggtga taaacaccag cagaaacaaa aaggctgaaa
    49501 tgatgatggc aatcaattgt ttgcctgcat tggtcctgaa aaaaggggtg gtggactcgg
    49561 tcagttctga ataaccggac gcatagatca tcatccagcc gatcaccacc aggcttaaaa
    49621 aggtggccag ggtgatccaa tccactttgg caaattcctt gttcctgctg atgatggagc
    49681 tcataaattc aggacttcct ccctgcggat gcgatcggac accagggtgg ccgagcgaaa
    49741 ggttttgcgg atttcattca ggctgcgcat ggcttcggaa tattcggtga acaccccgac
    49801 gcggatctgg taataaggat cgttgagcag ccatttggaa ctgtattggg gaaagcggct
    49861 ccggaaattg gacagggcgg actccatttc cctccggtcg gtggaggcaa agacctggat
    49921 cctccatccg gggaaatccg cacgtttcag gttgtactga ataaaatagt cgttgagcct
    49981 ggtgatggct gggctttcga tcacctgcaa gcgtccggtc tgcgcctggc caaggccata
    50041 agcaaaacaa agcatcagca tcaacttcat ggataatctc atattcagac taaatatact
    50101 ttttatcagg cattggcccc atggcactgt ttatatttct tgccgctgcc gcagggacag
    50161 ggatcattcc ggccgatctt ttcggttgcc cgcttcacgg tctgatgagc cggcgtgtct
    50221 tctccccggc tcaagatgta atcctgcttt tgtttttgcc gtatttcttc gacttcttcc
    50281 tcccgttgaa tccggacgag acacagcttt tccagcgtat ccattttgat ggtgccgatc
    50341 atttccatga acatggcata tccttcacgc tgatattccc gaatcgggtc tttctgaccg
    50401 taaccgcgca agccaatccc ctcgcgcagg tgatccatcg acaacaaatg atctttccag
    50461 tgagtgtcaa tagcttgcag cataatgacc ttcataagat aggtcatgag ctccggaccg
    50521 aattcttttt ccttgttccg caaatgtttc aacacatttt cgttcagaaa actcttgatg
    50581 tcttccggag aacgaagttc ctgagcgccg ctgatattca aatgtaaatg gtattgcttg
    50641 aacaacgcat catcaaggcc tttgaggtcc caattttc
//
LOCUS       DAOQXT010000023        50357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_761_length_50357_cov_6.470001, whole genome shotgun sequence.
ACCESSION   DAOQXT010000023 DAOQXT010000000
VERSION     DAOQXT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 50357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50357
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_761_length_50357_cov_6.470001"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKM27_05840"
     CDS             complement(<1..657)
                     /locus_tag="PKM27_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNR06820.1"
                     /translation="MAENLLFSQNEDAYKLLISRLDRKLDEIYEGGGKKRLEKQHAEG
                     KLGARERIKALLDPGSPFFEIGALAALDLYPEEGGCPAAGVICGIGRIHQRLCMVVAN
                     DATVKAGAWFPMTGKKNLRAQEISMENRMPIIYLVDSAGVYLPMQDEIFPDKEHFGRI
                     FRNNARMSALGIPQIAAVMGSCVAGGAYLPIMSDEALIVDKTGSIFLAGPYLVKAAIG
                     E"
     gene            complement(720..2222)
                     /locus_tag="PKM27_05845"
     CDS             complement(720..2222)
                     /locus_tag="PKM27_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNR06821.1"
                     /translation="MKPFLYILVTALASSMFTIFIYSKQFPQRILVREPRAVFSDSRS
                     GLPAWSSENPYVESNPLSTTSINKTNSAPDDPVSFRDAARKSTPAVVYIRSTEIRTNR
                     WGYEYLDHSSGSGVIISADGYIVTNHHVIETGSEDLSANIEVTLNNKQKFSAKVVGDD
                     PSTDIALLKIEAQNLPFLHFGNSDEVETGDWVIAIGNPEKLRSTVTAGIVSAKGRNIN
                     ILEDRDFSIESFIQTDAVVNPGNSGGALVGAHGLLVGINTAILTHTGNYEGYSFAVPS
                     NLVKKVIQDLKEFGKSQRAFLGITLNDDIDNQKVADLKLPDHSGVLVEKVFAQSAAED
                     AGLQAGDVLLRVDQIAISSNNDVHEKIGSKRPGDEVTLTFYRNGNTMETIVKLKDVNN
                     KSSIENVNVHAEKILTNQGFEIRDLNPYEKSRFKINGVRVVGIYKGSVIDMTNMQTGY
                     IITQINGQTVKDAADFISKFKQVNGYIDLTGFYDFDRSQSIYPYKFRKVS"
     gene            complement(2355..2681)
                     /locus_tag="PKM27_05850"
     CDS             complement(2355..2681)
                     /locus_tag="PKM27_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR06822.1"
                     /translation="MDRKETLLRFLEKDPASPFLLFALAKEYETEGNLSASLETYEQL
                     LQADPRYTGAYYHLGKLYERLESPDKAKEVYEHGLVLCRQQKAWHDANELQGALNDLL
                     PPDELG"
     gene            2770..3510
                     /locus_tag="PKM27_05855"
     CDS             2770..3510
                     /locus_tag="PKM27_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNR06823.1"
                     /translation="MKILVCISVTPDTTSRVSFKNDNREYNPDGVQFILNPYDEWYAL
                     VKALEIREKSGGSVTVIHAGPVQHEPMIRKALAIGADEAIRIDVRPDSSHAAALQIAA
                     AVKDQAFDLILTGKETIDYQGAELGAMLAEWLALPFVPFASKLDLEGSRAIIERDIEG
                     GVQVLEVQLPAVVSGAKGLAEQRIPNMKGIMAAKSKPLSVRDGVPGEDLILPAGFTLP
                     PPKQGVRMVAPEDMDQLVRILHEEARVL"
     gene            3529..4494
                     /locus_tag="PKM27_05860"
     CDS             3529..4494
                     /locus_tag="PKM27_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNR06824.1"
                     /translation="MSVLVFIETFQGGFKKPALEAVSYGHALSRLMQGQCQVLVYGKT
                     DQPGLPGRHGADSVLYLDDEDGLADTAWLSGIIAREAAKFGAKALILAQNSTSRGILG
                     RLAVSLKSGCASGVTSLPEIKEDRMEVSRSAFAGKASARLSLGGPCKVLSIASNAVLM
                     KTDGQDAPVEVLTRPEPSGKVKVLETKRISGEIPLAEAERVVSAGRGMKEPGNWGIIE
                     DLAHELGAATACSRPVADSGWRPHHEHVGQTGLAIKPNLYIAIGISGAIQHLAGVNNS
                     KVIVVINKDPEAPFFKAADYGVCGDLFEVVPRLTAAVKRYKEQHH"
     gene            4557..5141
                     /locus_tag="PKM27_05865"
     CDS             4557..5141
                     /locus_tag="PKM27_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HNR06825.1"
                     /translation="MKKIELEIVTLSHSISQSHNYAVVLGELEGNRKLPIVIGSFEAQ
                     AIAVAMEKMNPNRPLTHDLFKNTLDTFDIEIKEVVINNLLDGIFYARLICNRNGETIE
                     IDSRTSDAIAMAVRANCPIYTFDFILESAGVVLEETEEKPVAGVKKEKTGGTFNQYSI
                     DQLTKMLEEVLADEDYEKAAKIRDEINKRKETNQ"
     gene            complement(5145..7127)
                     /locus_tag="PKM27_05870"
     CDS             complement(5145..7127)
                     /locus_tag="PKM27_05870"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNR06826.1"
                     /translation="MPDSYRFQSILKSILLPSLILFGPRSVGQVPESLGLAGLLKDFL
                     EFHRLPSYQPDTYCAQVSSYDTTGGNDDGFSGKYSYLRRNPDSSLVLFDLKGPGVIQR
                     IWTPTPSDDSLDFFIDDTLRPAFTTRYRDLFSGEVYPFVAPLCGNQLGGFYSYLPIPF
                     SKHCRIVYKGKRTQFHQIQYRLYPESRTIKSFDPRLNPEEKKILDQLEKAWSADPTIA
                     LFSGNHPVTTYQKAVSLQPGGQTTLFDLARGGRIAGFELSPSSAFEGLHKDLDIRITW
                     DGENRPAVYCPVADFFGFAFGRVSMKSLLLGSSGDKNYCFFPMPFDRSARIELIYRRR
                     PGIVQQPVRIESTLWFQPVERNPALEGKFYSYYSSNTGLPEGRPHHLLEVYGKGHFIG
                     TTLQCQGLRPGMTLFFEGDDVCRIDGETRIHGTGSEDYFNGGWYALLDRWDGTYSLPL
                     SGSLDYSLPYCRTGGYRFFLADKISYETSFHHTIEHGPVQNNIPAAYTSVTYYYSDQA
                     GIPQPAPEAGSTRVHRPDTFVLYPQIASFSLDGDIRLKTSWSYPTGGLTYSFEIKPGS
                     VIRMSLAEIPEGEYSLDLDFITGPGACDFSIWQRQSRKSDWVQAGSVETKRIEQQEIT
                     QLRVDRLHQTISFHFKASDTKNQFILNRVVLRKRGK"
     gene            complement(7169..8863)
                     /locus_tag="PKM27_05875"
     CDS             complement(7169..8863)
                     /locus_tag="PKM27_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNR06827.1"
                     /translation="MSYFDWAILIFSLVGIVAYGIYKTRGPQDKDSFLKGNTEGRWWA
                     VCLGVMATQASGITFLSTPGQGYSDGLRFVQFYLGLPIAMLIICVTFIPAFFRSGVFT
                     AYEYLEKRFDLKTRLLTSFLFLIQRGLSAGITLYAPAIILSALFGWNFKLVILLTGIA
                     VILYTVSGGAKAVSKTQSIQMIVMLGGLLFIFFLILYKLKGISFDQALQIAGWSGKMN
                     AINFNFDLKDRYNFWSGIIGGAFVALAYFGTDQSQVQRYLGGQSITQSRLGLIANGLV
                     KVPMQFFILLTGVMVFVFYQVNGSPLFFNQQVEAKILSSNHASAYQEKQTALAEISKG
                     KQEVQSRLLQETDADPAMKTQLQNLLQQENTVRTEGKAIIKQALPDTETNDRDFIFLH
                     FVLNHLPMGLIGLLLAVVFAATMSSTSAEITALAGTTSIDWVHRLFKRRKLDDLMIIF
                     LTRVFTVVWGLFAIGFAMYATLFENLIQFVNLIGSLFYGTILGVFLCAFYLKYTRGHA
                     VFTAAILSEITVIVLYNTTDIGFLWFNLIGCGLVVLIALILSAFLPLQDKKLVIPS"
     gene            9087..9329
                     /locus_tag="PKM27_05880"
     CDS             9087..9329
                     /locus_tag="PKM27_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNR06828.1"
                     /translation="MRTKVQKWGNSLAVRIPKSFVVDAHLSPDCLVEIRLEDDHIIIK
                     PVAKPSWTLEELLAGINKRNVHREVYAGPAQGKEIW"
     gene            9333..9665
                     /gene="mazF"
                     /locus_tag="PKM27_05885"
     CDS             9333..9665
                     /gene="mazF"
                     /locus_tag="PKM27_05885"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: protein motif:HMM:NF007386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HNR06829.1"
                     /translation="MAYIPDRGDMIWITLNPQAGHEQSGRRPALVLSGAAYNKKVELA
                     ILCPITSQIKGYPFEVLIPDGLEITGAILADQVKSLDWKARKATFAGKLPESSLFEVI
                     GKLKTLIG"
     gene            complement(9788..12301)
                     /locus_tag="PKM27_05890"
     CDS             complement(9788..12301)
                     /locus_tag="PKM27_05890"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNR06830.1"
                     /translation="MKRLTALLPALFLVAFSVAQSPKKPTSAEIHQSLKKLNVLATAF
                     YVAAHPDDENTRLISLLANQYKADVYYLSMTRGDGGQNLIGSEIEDLLGVIRTNELLE
                     ARKIDGGNQLFTRANDFGYSKTPAETMKTWDRQEVLSDIVWQIRKHQPDIIINRFNDN
                     IERANHGHHITSAILSTEAFDLTGRKDIFPDQLKYVSPWQPRRLFFNTIWFLYGSQEA
                     FDKVDKSNMVVMDAGVYFPWLGKSNGEIAAESRSMHRCQGMGGLGNRGTSMEYLDLLK
                     GDKPTTLDNPFSGMDITWNRVKGGAALIPLIQDLDRTFQYDNPAASIPKLNKVYQMIQ
                     ALPDGYWKSKKSEDVKNLIAQCAGLFMEAVSNTHTVAPGEKTVVTAEIINRSNARVIL
                     ESVRFLPMEGADTAGAIILAPNMGWRFNRTVTIPENTKNTSPYWLTEPWEDGMYTVAD
                     QKLRGLPLTPRECKVQVNMTIDGTPFQFEREMVYKWVDPAKGELYRPFEITTPAFVNL
                     DSKVYVFADDAPKKVTIQVKSGISALKGKLALRLPAGWRTEPASLPVDIKTKGNQQSY
                     SFLLYPPTGSQEAKVTAEIDIDGKVYSKELVTINYDHIPFQTVQRTSEARLLRLDIKK
                     GGTQIAYINGAGDDVASSLSQVGYQVTVLTNEQSLSAEQLTKYDAIVLGIRAYNTNEK
                     LKELHPVLMDYVKNGGTVVVQYNTNFRLVSPELAPYALKLGRERVSEEDAEMRFLKPD
                     HPVLNVPNKLNQKDFSGWVQERGLYYPTEWDKEHFEAILSANDAGEPPRDGGLLVAKY
                     GDGHYVYSGLAFFRQLPSGVPGAYRLFANLLALKKHPKS"
     gene            12762..13325
                     /locus_tag="PKM27_05895"
     CDS             12762..13325
                     /locus_tag="PKM27_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06831.1"
                     /translation="MASSPSSPDFSSLLLRVNNYKRILQNTIEYRKAWPTELKPLIES
                     VLKEFLAQSGLKGEVRVQDRIENLESVVLDLGKSHSGISENLDDTDIKRTMIKSNGTL
                     IYQQLFNGKIMVMVLNPHIEGYGEPKAPITLEILRPEELKAGFMLRHLETFLKNITEW
                     EDYDDDLPTKTPIGFQPVGFQVDVPNE"
     gene            complement(13374..13781)
                     /locus_tag="PKM27_05900"
     CDS             complement(13374..13781)
                     /locus_tag="PKM27_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06832.1"
                     /translation="MKKIILKGLTAGTVLLLFSYFSLFLLVKIFPHLAEEYYDPMFSL
                     EGSKAWTFFVHPYIISFVLAWFWNNFKSMFTGSFWLRGLKLGLVYALVAILPGTWMIY
                     SAFSVSVPMVLSWGLYGVIQAAVAGIICARLSP"
     gene            complement(13800..14435)
                     /locus_tag="PKM27_05905"
     CDS             complement(13800..14435)
                     /locus_tag="PKM27_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNR06833.1"
                     /translation="MILRFFLLISGLFTRVFAPEDLLEGRFRILPESKVFLAGTTNVN
                     KFKCDCGNTFDPQKFQLDLNPQQLMDFTGTSLQLPVIYFDCKNGKMDRDLQKALKAEQ
                     YPYIGIELKQFSMYPGEIPLSGSDEGLPSLAKVNITIAGVTRSYEIPVRTTRDAHLQF
                     RFTGKKTMNMTDFNISPPVALFGMIKVDDPITFHFNLLVHAEVLPGKTLLQ"
     gene            complement(14488..15846)
                     /locus_tag="PKM27_05910"
     CDS             complement(14488..15846)
                     /locus_tag="PKM27_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06834.1"
                     /translation="MKNSSTLLLLALLLISTSLFSQSDIQYSRPWNKEGINIFEPVKS
                     SDQPAYEGFKLRIGGSFTQDYQHLKHSNTPTKSANYLYGVVKAEDSTSALLRGFNLAM
                     ANLNFDFQIEDGIMVRLENYMSSRHHSEFWVKGGYVQIDKLPMFGNPQWFTDNFRVKI
                     GHFVPNFGDMHFRRTDGGNAMFNPFVENLIFDAFTTEIGAEVYAWPTKSIMAMVGMTS
                     GYINGNVDYFPTSNNSSGVVPTKKTPSIFAKLAYDNNFEDLRFRLSASVYTNPNISRN
                     TLYAGDRTGSHYFLAMEPALTNGAATTMAAQFTSGRLNPDMANRITAFMINPFLKYKG
                     LEVTGSYETLSGSVYGDVKEGKWVKRNSSQYSVEASYRFLANESMYIGVRQIGAKAEL
                     RGITSDVNINRFAVAAGWFPTKNLLLKAEIVSQKYKDFPSTDYRYNGKFNGAVIEAVV
                     GF"
     gene            complement(15882..16445)
                     /locus_tag="PKM27_05915"
     CDS             complement(15882..16445)
                     /locus_tag="PKM27_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNR06835.1"
                     /translation="MKIQKITNLLLILLLPVFVCSQESYRISLTKVIIKGTSNVHKWE
                     STVGQLSGTATLTVDGEQLKSLNQARFDIPVKSIKSPKGKIMDNKTYDALKSEECANI
                     SFLLSAPSPDGSGNLNSKGSLTIACSTKAIDLTSTVKSLGNGEFSISGSKKMKMTDFG
                     IKPPTALLGTMTTGDEITIEYEVKLVK"
     gene            16846..16919
                     /locus_tag="PKM27_05920"
     tRNA            16846..16919
                     /locus_tag="PKM27_05920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16880..16882,aa:Arg,seq:acg)
     gene            16972..17220
                     /gene="rpmB"
                     /locus_tag="PKM27_05925"
     CDS             16972..17220
                     /gene="rpmB"
                     /locus_tag="PKM27_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNR06836.1"
                     /translation="MSRTCDLTGKRPRKGNHVSHSNRKTKRHFYPNLQDKRFWVPELN
                     KWISLRVSVSALRTIDKVGIYQFIKSQKAKGLAQGVRI"
     gene            17241..17429
                     /gene="rpmG"
                     /locus_tag="PKM27_05930"
     CDS             17241..17429
                     /gene="rpmG"
                     /locus_tag="PKM27_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNR06837.1"
                     /translation="MAKKSKGNRQQIILECTEHKNSGLPGTSRYITQKNKKNTPDRLE
                     IKKYNPIMKKYTIHKEIK"
     gene            17432..17605
                     /locus_tag="PKM27_05935"
     CDS             17432..17605
                     /locus_tag="PKM27_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HNR06838.1"
                     /translation="MAKVSKNAKVAQRAANAGSGKDFVKVIKSVKDPVTGKYSFKEMI
                     VHKDKVKEFFANK"
     gene            17679..18632
                     /gene="ftsY"
                     /locus_tag="PKM27_05940"
     CDS             17679..18632
                     /gene="ftsY"
                     /locus_tag="PKM27_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNR06839.1"
                     /translation="MSFFNKFFTREKKEDLNQGLEKTKTSFFTKLTKAIAGKDKVDEE
                     VLDELENVLITSDVGLPTTVKIIERIEARVARDKFLNTAELNGILRDEIVNLLSENNT
                     VDLEDYDLPAQPRPQVMLVVGVNGVGKTTTIGKIAFQHTIRDKKVVLGAGDTFRAAAV
                     DQLKIWSERAGCLFFSKGMNVDPAAVAYETVDFALSHQCDLAIIDTAGRLHTKLNLMN
                     ELGKIKRSISKRLEGAPHEVLLVLDATTGQNAIEQAKHFSEATDVTAIALTKLDGTAK
                     GGIVLAISDQFKIPIKYIGVGEGLDKLQIFNKKAFVDSLFE"
     gene            complement(18734..20332)
                     /locus_tag="PKM27_05945"
     CDS             complement(18734..20332)
                     /locus_tag="PKM27_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR06840.1"
                     /translation="MSISAIGPVFSQGNSCNTKYPSCYSFIGNPFSTNHTFPCWKLFL
                     SDDKINIIDISFTQPDFGFILSNNSIYQYQNSSSTNIYKLPFEIFSTSRIYFINAKTG
                     YVIANYFNGFFIIKTTDGGLNWSLVYSNTLISESIRSIYFHNENKFFIGLDRSILIFK
                     DDKLEKYKNKIFSSTVDRLVGICYISNTLIIASQNELYYSKEEPYNTLIKYPNFRSNI
                     HFLTFSNNQLFIYVSNGLYRGEQFDKLIFNKYLMAQETSPGIFAVLDTNTFWMGDGLS
                     IDGGCTANHNYYSFDRIITFGHHGAIGLYHKNLYNYSCNNSNRIYDAPIYSQNILLPE
                     KDSFIFNFKTDLTSHYLSNLPLPSCWLSSKNLDYPYFYYFKGNGKSYSFRVTSNAPSQ
                     VAILSGNISCLKEKTCSEINVFNKNIYMVLNTIENDDYYILIDHSIKGNGQLIVSNQK
                     TVPLKYLQKGSLKADIYGDIISTQDDNKSTIYVYTIDGKLFAEKYFYRNINLREFNLA
                     KGLYFIVVRSGMSFKVFKYNSIQV"
     gene            complement(20493..22112)
                     /locus_tag="PKM27_05950"
     CDS             complement(20493..22112)
                     /locus_tag="PKM27_05950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HNR06841.1"
                     /translation="MNKYRSTLLLFLGLACFQVRAQQDWIIQLWDGKSDSDLHSGWSK
                     SPLRSRQDIKTQNIAPHFNIYTLKAGAPLNKKDLLANPAVRFAELNLALETRSTPNDP
                     LINQQWHLDNISASKAWDVTKGGLNFNNDTIVIGVIDHGFLTSHEDLKNRIWQNHAEI
                     PGNGKDDDNNGYVDDVYGLSLKYKNEKHPADNINDGLSNHGTSVAGLIGAESNNSKGV
                     TGMMWNNKLMIATFGTNNIGELIEMFNYMLDQRIRYNRSGGKEGAFVVAINYSGGISF
                     AKAADYPIWCGMYDKMGLEGIVNCGATTNKYADVDEEGDMPSTCPSDFLIAVTNTGKD
                     NRRIYNAGFGLNHIDLGAPGEGIYSTQSANINAYISFSGTSAATPVVSGAVGLLYSYP
                     CREWADYQKANPADAARLVKRALLASVDKNSDLSGKTVSGGRLNIARALDTLNSYFCQ
                     TLNSSKLKINLIYPNPTNSILNLKINVNSTNKYFIKIIDGLGKTILNANLWSNQNLIT
                     LTIPNLSEGIYYGILYDNFSSCTFKFLYKNL"
     gene            complement(22448..23053)
                     /locus_tag="PKM27_05955"
     CDS             complement(22448..23053)
                     /locus_tag="PKM27_05955"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNR06842.1"
                     /translation="MRGIFAVLLILLFLPMYGQILSYKEEIRLYRSKNWAGLFSDPRT
                     TVKPGDSVYLGYYPVRESNKVFCEVELLQDQPSFQMPTYSGQEKTFRRHAVLHFQWKG
                     KKLRLTAYKNLHLVSPLYQNHLFIPLKDLTTGKKTYAGGRYLDLQATAIQDGRLILDL
                     NRVYNPWCAYSDGYNCPIPPVENHLKVALKVGEKDFKKPKS"
     gene            complement(23050..23937)
                     /locus_tag="PKM27_05960"
     CDS             complement(23050..23937)
                     /locus_tag="PKM27_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06843.1"
                     /translation="MFIRRWPLLFAFLLSRLEAQVPATQLYEFRIQKGETGKFVVHSP
                     RYLSAFNEGGYNNQPYFINDYEILITVQEKGAGQTDIFKLNLGLNTLQPLTRTSLSEY
                     SPTLTPDGKYISCVRVDDQATGLQRLYRYERKPKGKAISPLPDIKNVGYHAWLDTQNV
                     ALFLVGKPHQMALVNLESKDPLLFTSDVGRCMTRNAAGHLVYVHKISESYWYIKEYDP
                     LMQKSVILSETLPGCEDFALSAEGHFFMCKGSKLYFMHPETEKNWVEIADLKYFGLES
                     VSRMALRGDRLILVNQPAQ"
     gene            complement(23937..25496)
                     /gene="lepB"
                     /locus_tag="PKM27_05965"
     CDS             complement(23937..25496)
                     /gene="lepB"
                     /locus_tag="PKM27_05965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNR06844.1"
                     /translation="MFLFLLIGFIIYSVGMYFVFPKAGVEAWKGLVPGLNLAEMSALV
                     GRKKSHAWWMLFPIVNIFIFAALIIDLARSFGKHSFFQSVLAVVFAPFYSLYLGLAKN
                     EKYQGPILMKEHEFYAKLKEAQDKKDQKTVNKLQASNPYKKGALREWAESIIFAVFAA
                     AFIRMFLIEAYMIPTSSMEGSLKVGDFLFVSKAHYGIRLPMTFVMVPLLHNRIPFLDT
                     ESYLKQPSFKYKRLPKLQEVKRYEPFVFNWPVGDSVYLAPDRAFTADQYRREPYVRQA
                     VRNAKLVTRPVDKKDHYIKRCVALPGDSLKIINRQLYINNQPAYNPEHMQFMYRVLSP
                     GNQLNQRKLDELGINLSEAMPQMGVYFLDSTQRAKIQAMDSSIIIQPVPQEARPLFPY
                     DTPLSLGWTMDDYGPIYIPKAGTTTSLTLENLPLYDRVISVYEKNKLEVRDGKIYING
                     AEASSYTFEQDYYWAMGDNRHNSEDSRAWGYVPADHIVGKPLFIWFSTKNGKLANGIN
                     WNRLFTSANKK"
     gene            25764..26246
                     /locus_tag="PKM27_05970"
     CDS             25764..26246
                     /locus_tag="PKM27_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06845.1"
                     /translation="MVLVVDLDGTYLKNDFFAERFYKRLIENPFFVVYHLFTGGILQL
                     KNLLLDKGRAEYPLDPLINSEVEKFILANRRNYERVVLVSASPDFFVKQVLSENEIFD
                     AVHGSVEENLKGEKKLEFIRNRFGEDFHYIGNHGVDIPILRAAKKGFIVKENRIHEYP
                     "
     gene            26233..27102
                     /locus_tag="PKM27_05975"
     CDS             26233..27102
                     /locus_tag="PKM27_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HNR06846.1"
                     /translation="MSTLRFSILRLLRPLHWVKNGLIFLPMLLAGSITRVPVLVDAVI
                     GFLSFSLMASAGYILNDIVDVDRDRRHPYKSNRPLAAASLSTGRALGVAVLLLLLSVS
                     LSWPLGMRAVGWLMVYFTLNWLYSAYLKPVRFFDIVILSSFYLIRLFYGSAIGDAELT
                     GWFVVASLFSFISLSANKRYLECAISKHGTLAGRGYSKDDAPMLQTLSVAFALGTLVL
                     LNLHAFFVLSIREPVVIALINLFAAGMMFSYFGNNKSKSDDPVARILSNPFLLAMALM
                     LMSIYVFELMQQR"
     gene            27099..29120
                     /locus_tag="PKM27_05980"
     CDS             27099..29120
                     /locus_tag="PKM27_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR06847.1"
                     /translation="MKTERINLFNWGGYPHHNCRIYHPETVEEIQELVRSKHSFIARG
                     NGRCYGDSALSNRVISTLALNRILDFDPISGLIVCEAGVLLDTVLKKIVPAGFFLPVS
                     PGTKFISIGGAFASDVHGKNQQVDGVFSDHVSYIKLVEPGGELVHLTPSDDLFRQTAG
                     GMGLTGIIVEVALRLRRIESQMISQRSIKAKNLAEVFDLFEQNSGSTYSVAWIDGMAR
                     GYKLGRSILLLGEHAAQTDVANLKTRELHVNSKLNLPFYFPSWVLNPLFLKVFNWAYF
                     TRPGSSGNGIIHYDSFFYPLDKIRHWNRIYGKKGFVQYQFVLPGAVAYEGIKHVLEVL
                     SINQLWPYLAVLKRFGTSHEGRYLHFPMEGYTMSLDLKISQRLWAVLNQLDEVVSSLG
                     GKTYLAKDARLGGKNFRKQYPVMPSIPPNPVFISHQQVRFQQMKKHVFLILGANSDIA
                     KQTALLYLKKYPEGYLILASRDVQGLRAFIEESGIGGCSEAVPLDVENFAGHALFYSA
                     LSAKPNWVMYAAGVLYTNDECMGDAPKSARNTSVNFSAPVNLLNIAVDDNNPGLQRII
                     GMSSIAGLRGRKSNFQYGSAKAGFHQYLFGLRQDLKDRGVVVQAITPGRVNTKMTAHL
                     PVHKRANTPEEVASAILQNTTTFEVYPNLFWRLIGMVVKYAPEVLIRKL"
     gene            29157..30269
                     /locus_tag="PKM27_05985"
     CDS             29157..30269
                     /locus_tag="PKM27_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR06848.1"
                     /translation="MCNFRPKTYSASGAMMWLPLLLLSSPAFLQSQEYTKILLNEFNP
                     KSIYKIPVSRVEKARYPAIDFHSHDYPKSDQEVDAWVKTMDACNIVKSIVLSYTTGPA
                     FDAVIKKYSRYPDRFEVWCGFDYTGMNEPGWSERAIRELERCHALGARGVGELGDKGL
                     GEYYSKPTPGIGMHVDDPRLRPLLKRCGELNMPVSIHVAEDAWMYEPADASNDGLMNA
                     AKWHVDMNVPGKLGHDALIQTLENAVRDHPGTLFIACHLANSCADLGRIAGMLDRYPN
                     LYTEMAARFGEIAPIPRQTKAFMEKYSRRILYGTDMGTSENMYRTTFRILETADEHFY
                     EKDRFNYHWPLHGLDLSDKTLKRIYALNGKRILKRR"
     gene            complement(30580..31098)
                     /locus_tag="PKM27_05990"
     CDS             complement(30580..31098)
                     /locus_tag="PKM27_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNR06849.1"
                     /translation="MVTKSIQDALNKQVCMEAESSQAYLAMAAWAEIQPGLEGITNFF
                     YQQSDEERMHMLKLIRYINERGGQALVPAIEQPMVSFKSIKHVFDEFLQHELKVSSSI
                     NELVHLSLAEKDYATHNFLQWYVNEQMEEERVARNLNDKLTLVGDDKGGLYLFDRDIL
                     NLRAKQAVAPAE"
     gene            31265..32332
                     /locus_tag="PKM27_05995"
     CDS             31265..32332
                     /locus_tag="PKM27_05995"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNR06850.1"
                     /translation="MPKVTFNTQHKSFYSSLQKKVETYFKERRIKKTGDQRLFFKAFL
                     LIFSAGLVYVLLLTVRLHPVAGLLLCGLMGFLLATIGFNVMHDANHGSFSDKRWINEA
                     LSLTANLMGVNAWLWRQKHNLIHHTFTNLDGVDEDLATTSLLRMGPYQKKYKVHRYQA
                     FYCIPVYGLTSLMWTFFSDFKKYFTRKIQVTALSEMSYGEHIIFWSSKILYAFLFIGL
                     PVYLLGFLPFLAGFLVMHLVLGITLAVVFQLAHVVEATHFEDAHKEDLHIGQEWAVHQ
                     VRTTADFATGNPVISWLTGGLNFQVEHHLFPRISHIHYPVIQRMVKETCADFGIRYNG
                     YPTMWAAFRSHLRLMKQLGIQ"
     gene            complement(32933..33808)
                     /locus_tag="PKM27_06000"
     CDS             complement(32933..33808)
                     /locus_tag="PKM27_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HNR06851.1"
                     /translation="MKIIFPLFWLLAIGPSSFGQVLSDSIRIGPHFRSFHFIQPSANV
                     SGASLVFVLHGSGGNGRALMERTLNFIEEMKTENAVLVYPDGYKNYWNECRKEASSLA
                     NKENIDEEGFFSAMIAYFKVRYGIHDRKVFAVGTSGGGHMCYKLAMTLPGHFRAITAI
                     IANLPDAGNMDCLEAGKAIPVMIVNGTHDPTNPYAGGMMQSANFRMGMVRSTEASFRY
                     WAGLAGYRGDPLKTTLPDADPEDEKTIERYTFKEKGKPEVVLLKVIGGRHDYPGDIDV
                     HWEAWQFFKRQIEED"
     gene            complement(33851..34531)
                     /locus_tag="PKM27_06005"
     CDS             complement(33851..34531)
                     /locus_tag="PKM27_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR06852.1"
                     /translation="MNRPYFLIWILILPLLVSGCKSMSKSQKGAVIGTAGGAAAGAVV
                     GKLAGNTVLGAIIGAAVGGVAGAIIGRKMDKQAEEIKEEIPDAKVVRVGEGIVVEFNN
                     KILFGFDRSDLSPESLKTMNQLVGILKKYPDTNIEIQGHTDSKGGAKYNQSLSIRRAS
                     SVSAHLSEHGVLPARLALKGFGESAPKYSNDTEDGRAQNRRVEFLIYANEKMIADAKK
                     EAAESGGK"
     gene            complement(34720..34875)
                     /locus_tag="PKM27_06010"
     CDS             complement(34720..34875)
                     /locus_tag="PKM27_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HNR06853.1"
                     /translation="MGNLHFLLAAILVMGWLIGFIGFRAGSLIHILLVLALITVLWRM
                     IRGKRTV"
     gene            35203..35808
                     /locus_tag="PKM27_06015"
     CDS             35203..35808
                     /locus_tag="PKM27_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR06854.1"
                     /translation="MNLYLKFEVNSLFQKVLENELKGLPVSYTVPGPGELTFHELVPD
                     EVLEAIRARLDQYGIEILENPKAVLIQKIKDAIIELVYREDSLARCKVSVYLSERLRF
                     GYGYLSKLFSESTFTSIENYLILQKTERAKHLIASQALTFTEIAWKLNYSSVAHFSTQ
                     FKNTTGLTPTAFQRIINKKRAVAAPGQNGVQLSEHPVDNAF"
     gene            35818..36264
                     /locus_tag="PKM27_06020"
     CDS             35818..36264
                     /locus_tag="PKM27_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR06855.1"
                     /translation="MQNLPISVLLADDDEDDRILFQEALNEIKFKTEVRTVGDGRSLM
                     EYLNRPEVKLPQVIFLDLNMPLKSGMECLHELKSSARFGNIAVAIYSTSASEEDIEET
                     FVRGANVYIRKPDDFNELKRILEKVITINWQYHTSGLNRENFLLSF"
     gene            36275..38068
                     /locus_tag="PKM27_06025"
     CDS             36275..38068
                     /locus_tag="PKM27_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNR06856.1"
                     /translation="MFQDLRSVFTSSFFLNIIFVSTLFLLIFISGISYRYTTALTDSS
                     RLVVQSRKTATELGHLSSLLEEAQSRHAAFILTGDTSLIRPYLHAGVKTGPVLQSLKL
                     LAGDNSQQIQHLDTLSRLIGHCLDLMARTLEWNTGLAADQARFKESLMLGVKEMERIR
                     VKIKDMLDFDSMHLKVHQDIYGREISLSPLYILFVMLFSMLVFIFAYFRINKDLKLEK
                     ANNEKLLLANESMNHAEEIGEFSSWQWHLQTNAFDFSDNQYRLLGYRPFEFEPNLDKF
                     LEFVHPEDRHKLKAARREMFSGPQNPLSVDFRIYRKDGSLRYFRSIAKLFTDGDRRPV
                     VIGIHWDITHQHLNRLALEERNLELEQSNKELATFNHIASHDLQEPLRIIQTYVSRII
                     DSEQARISETSQGYFSRILAALSRMRALINDLLLYSRTTKTEKVFQLSDLNQIFENAR
                     QELAQVLEDKQAELSSDPLPEAKVIPFQIQQMFVNLIGNSLKFSRLGVKPRIHLSCSE
                     VPSDPYREHHTGPVKKFYKLTLTDNGMGFEQQYARKIFDLFHRLHGRDEFTGSGIGLS
                     ICKKIAENHGGEITAEGRPGEGAAFTILLPH"
     gene            38213..38866
                     /locus_tag="PKM27_06030"
     CDS             38213..38866
                     /locus_tag="PKM27_06030"
                     /inference="COORDINATES: protein motif:HMM:NF017758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HNR06857.1"
                     /translation="MKNPSHQQARANQPPSVLPLAIPAHSTIAEGTSAHGTRTQGPKA
                     HGLYKLYRSQLFDMYWAEKALLKQFPKMIRHAHAHKLAETLYILLDSTRKHINRLEAL
                     FSSSNLRPVENRCKAMDGLIKETTEVIQSTEVGYVGDAGILSSGQKMEHYGMAGYQVL
                     QALAETLGEARAAAMLKYTLEEKKQAAEVLAGLAMDYLVENEFTGTGDAGDRYDLHP"
     gene            38884..39366
                     /locus_tag="PKM27_06035"
     CDS             38884..39366
                     /locus_tag="PKM27_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HNR06858.1"
                     /translation="MVRKNSNMENQEIIKAIRTLIQVNDDRIQGYESAMKESKEADLK
                     SNFFRFIQSSRRSKTQLVRVLIQSGASPVRESRQNGLFYGMLMNLKAAVRGRDRLSIF
                     RCWEKVEDLVAEMYERILQNEGFDWPPDYAGIIGSQRSVLEADRNKVKVFREILTEEN
                     "
     gene            39439..39732
                     /locus_tag="PKM27_06040"
     CDS             39439..39732
                     /locus_tag="PKM27_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNR06859.1"
                     /translation="MNSMKSVLIALAGVALGAVAGTLLAPARGFVTQRKLAKKGGEYA
                     EDLESKFNEMIDQFTQKFRTLKNDLVRKSENGKSEPEDSLVQSGSLSGRNKFQ"
     gene            39970..41097
                     /locus_tag="PKM27_06045"
     CDS             39970..41097
                     /locus_tag="PKM27_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNR06860.1"
                     /translation="MNISLKWPFYAKISLLLIGIYVFISILSAIQDIVLPVIYGLLLA
                     VLIGPLVNKLEARGMPRSLAIFSILILFVAGFIFLLVLLSSQASLLSEAFPVLSAKFD
                     ELVQQGVAWGSDFFNIRASKINQWVKSIRGDIYESSSAVVGATLTTAGGVLATLFLTP
                     VYVFMLLYYKSHLIEFIYRLMGRHHDQKIREILSETKTVIQGYLHGLLAEFWIIAVLN
                     SAGLLILGMDYAILLGIGGALLNVIPYLGGLIAVVIYIVVALVLKTPVYALYVFILYV
                     VVQLIDNNYIVPKIVGSKVKLNALVCLLSVLAGSALWGIPGMFLSIPLIAIIKLVLDR
                     IPGLSPWGFLLGETRAGGGIRKRTAARPTGPVPEGSLEVRL"
     gene            complement(41140..41724)
                     /locus_tag="PKM27_06050"
     CDS             complement(41140..41724)
                     /locus_tag="PKM27_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNR06861.1"
                     /translation="MKINAFLIVTTLLTAGNLSAQHVNFGIKAGLNVFTIHNETNTNS
                     DPLAGFHLGILGHIHLDRSIALQPELLYSVQGAKSSGQNVGLKTELDYLNLPVLFQYM
                     FDNGFRFEAGPQLGLLVGAKSKVNNLSTDIKENLESLDAGLLVGLGYIHPPTGFGIDA
                     RYQFGLTNINKSENFKSYNRGFQLGVFYQIKHRD"
     gene            42002..42157
                     /locus_tag="PKM27_06055"
     CDS             42002..42157
                     /locus_tag="PKM27_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06862.1"
                     /translation="MNWISVFFGLIALVLIIYLVIRNQKDRKEFEKILNTDYKKTKDE
                     EDEMDDE"
     gene            complement(42193..42453)
                     /locus_tag="PKM27_06060"
     CDS             complement(42193..42453)
                     /locus_tag="PKM27_06060"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNR06863.1"
                     /translation="MDKHTKESFEVHGENLLKKVKELIQEGNIRKISIHKKDGEEIIS
                     FPLTLGVVGTLFAPVLAAIGAMAALIGDCTITVERLDKEEKE"
     gene            complement(42655..45210)
                     /gene="mgtA"
                     /locus_tag="PKM27_06065"
     CDS             complement(42655..45210)
                     /gene="mgtA"
                     /locus_tag="PKM27_06065"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNR06864.1"
                     /translation="MDKNKPFSQIPLEELYRILDTSRNGLSLVQAGVRIRLQQQRLKT
                     ESRARREIRLLFRQFFNPLVLLLLLAVILSAWIGEASDTILILFILLASGLLGFFQEL
                     SAGRALEKLRSLIEMKHDLLREGKVVQVPTQEVVPGDVLILNAGDVIPADCRIIENSE
                     LHVNEATLTGESFPAEKEVGQLDENKPLHEKTNCLWKGTNVISGTARALVIHTGENTL
                     LGQLSQSLSRITETAFEKGIRNFGYFILRITVLLSLVILTTNLIFDKPVFDSILFALA
                     LAVGMAPELLPAILTFAMSVGAKRMMKKKVIVKKLSSIFNFGEVQILCMDKTGTVTEG
                     SVQVHDIVDHEGKPDPRVRLFAGLNAGLQAGYQNPVDEALRSLALDFKPYSKSNEVPY
                     DFIRKRLSVCVKHPNGNLIITKGAVSSILEVCRFVQSGAEIKDLDEVSRQNINALFKE
                     YSLKGFRVLGICIKTLHREKMRREDENEMIFSGFIILEDPLKAGSLESIQKLKKLNVG
                     IKIITGDNRHAAAYIANKLGLPDNAIITGDQMHTLSPEALTVKARSTFVFAEIEPYQK
                     ELIVKSLQKSHLAVAYLGDGINDVAAIHAADTGISTNNAVDVAKEAADFVLLEKDLSV
                     LSDGIVEGRTSFANSMKYIFITTGATFGNMLSVATASLLLPFLPMLPKQILLTNSISD
                     LPFLTIASDEVDKAELVAPRKWDMKLIRKFMVVFGIHSSVFDFITFYTLYYYFKLTGP
                     AFQTGWFLESVLTELIIIFVIRTKKSFIRSKPGNLLLAFGSLAFILTIWLPVSPFAPA
                     LGLSLAHVQQVIALLIIMLAYTLTADILKIFFFKYVKTEDTNPVVKELPVQKE"
     gene            complement(45416..46054)
                     /locus_tag="PKM27_06070"
     CDS             complement(45416..46054)
                     /locus_tag="PKM27_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06865.1"
                     /translation="MTLNIPEMRWAGILLLAGGLIFWIGAFSPPYKHWMTKDVREYLS
                     IIHGHKPNWYFIHGAFTVGVLLTLFGVQLLTHAMTRAGAGVYSQLAFTGFLFGSLFWI
                     MNIAFRLTVTVWAADKLSTSTILEPSFKTWMDWTNLLFAIYMVLAYLAIGAMGLAAKD
                     LDLLPPWVTSTCIWFGFAGAALYFLRFPVFEPPLMVHTPLILLGLVILLTIK"
     gene            complement(46185..46523)
                     /locus_tag="PKM27_06075"
     CDS             complement(46185..46523)
                     /locus_tag="PKM27_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06866.1"
                     /translation="MNTRSKLILVKIIHTAIWLFFNVVIFYMLYAALTDKLDRWLWIG
                     FGLVFLEGLTLVLFKFYCPLTLVARKYSDSGMDNFDIYLPNWLARNTKRIYTSLVVFI
                     LVITLYRALT"
     gene            complement(46562..47764)
                     /locus_tag="PKM27_06080"
     CDS             complement(46562..47764)
                     /locus_tag="PKM27_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNR06867.1"
                     /translation="MPSPSIPVIRRIESVDVLRGIVMIIMALDHVRDFIHIGAFTDDP
                     LNLATTTPVLYFTRWITHFCAPVFVFLSGVSIYLQSFRKTKKELSVFIIKRGLWLIAV
                     DLFIMSLVISFNPNYNYFFLQVIWTIGISMVLLGLFIHLPFKLILALGLVIVFGHNLL
                     DRLERAEGFDPGFAWDLLHTGNFALYPLWGNHTLVIIYPFVAWTGLMMLGYVMGSWYS
                     PDYPAEKRKKMLTRFGLAAIFLFIVLRYLNIYGNPFPWSPQKNLFYTLLSFLNTHKYP
                     PSLLFICMTIGPSLLMLAWLESLRNGFTRTVQVFGRVALFYYIAHFFLAHLVSAVLFL
                     ARGHSLAEADDQIRNFPFYFLVPGEGLSLPWVYLVWLGIVLFLYPLCKWYNDYKSVHK
                     EKWWLSYL"
     gene            complement(47915..49384)
                     /locus_tag="PKM27_06085"
     CDS             complement(47915..49384)
                     /locus_tag="PKM27_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HNR06868.1"
                     /translation="MSNLKDILTLGELKRSGYIPKPIKDEMRDNLVHALKNKINIFEG
                     IHGFDDTVIPDLQRAILSRHHILLLGLRGQAKTRLARLLVRLLDEYIPVIAGSEINDN
                     PFAPLSPYGREEVRTMGDQTTIEWVHRDQRYVEKLATPDVSIADLIGDVDPIKAASLK
                     LPYADERVIHYGLIPRANRSIFVINELPDLQPRIQVSLFNILQEGDLQIRGFKVRLPL
                     DVLFLFTANPEDYTNRGSIITPLKDRIDSQILTHYPTDIGTAMTITAQEARVKPEQKN
                     IFIPPLLKVLLEQISFEARTSELVDTNSGVSARLGISAYENLVSAVERRMLINNEKSG
                     TARLSDFVGVMPAITGKVELVYEGEQIGPYKVAFELLNKAIKSEFLKNFPHPEKQKKN
                     ERDPFGVIKAWFAGEHTLKLAVDASQEEYEASLNQVSGLERLVKTLPIPDSDKPVYKE
                     LVLHGLSAFNLLTKELHQTRFDFSDPLSDFKEKEEDLDD"
     gene            complement(49381..>50357)
                     /locus_tag="PKM27_06090"
     CDS             complement(49381..>50357)
                     /locus_tag="PKM27_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNR06869.1"
                     /translation="SWLTELDKRFKLTTEEYGIADFIRDLIDKGYINENREGIINPMP
                     SSKLEIALRRQALDDIFDQLKKSRKGQHTSTRTGLGDETTSEIRPYEFGDRLDQIALT
                     ESLKNAQIQHGIEDLVLREDDLEVYETYFQSQTSTVLMIDISHSMILYGEDRITPAKK
                     VAMALAELITTRYPKDTLDIITFGDDAKEIAMKDLPYLQVGPYHTNTVAGLELAMAIL
                     RRRKNPNKQIMMITDGKPTCIKVGKKYYKNAYGLDRKIVNRTLALALTCRKLNIPITT
                     FMVARDSYLASFVDAFTRANNGKAFYSTLDTLGEFIFMDYKKSKKKRR"
BASE COUNT        12742 a        11764 c        12905 g        12946 t
ORIGIN      
        1 ttccccaatc gccgctttta ccaggtaggg accggcgagg aagatggacc ctgttttatc
       61 cacgatcagg gcttcatcgg acatgatcgg cagataagct ccacccgcga cgcaggatcc
      121 catcacggcc gcgatttggg gaatgcccag ggcagacatc cgggcattgt tcctgaaaat
      181 gcggccgaaa tgttctttat ccggaaaaat ttcatcctgc atgggtaggt agacccccgc
      241 actgtcaacc aggtagataa tgggcatgcg gttttccatc gagatctcct gggcccggag
      301 atttttttta cccgtcatcg gaaaccaggc tcccgctttt accgtggcat cgttggcaac
      361 gaccatgcac aacctttgat gaatgcggcc aatgccgcag atcactccgg ccgcggggca
      421 tcccccttct tcagggtaaa ggtccagggc ggcaagggcg ccgatctcaa aaaaagggct
      481 gcccggatcc aggagggctt taatcctttc ccttgctccc agctttcctt cggcatgttg
      541 tttttccagc ctttttttac ccccgccctc atagatttca tcaagtttac ggtcgagtct
      601 ggaaattagc aacttgtacg catcttcatt ctgggaaaac aagagatttt cggccatgag
      661 cggttatttg caaatggaag ggcagaaggc gcccctgcgg gtcaaatgta taaaaaagat
      721 caggagacct ttctgaattt atacggatag atcgactggg aacggtcaaa atcataaaag
      781 ccggtgaggt ctatataccc gttgacctgt ttgaatttgc tgataaaatc cgctgcatct
      841 tttacggtct gtccgttgat ctgggtgatg atgtacccgg tctgcatatt ggtcatatcg
      901 atgaccgaac ctttgtaaat gcccactaca cgcaccccgt tgatcttaaa ccggcttttt
      961 tcgtaggggt taagatcacg gatctcaaac ccctggttgg tcaggatctt ttccgcatgc
     1021 acatttacat tttcaatgct cgatttattg ttgacgtctt ttagtttgac aatggtttcc
     1081 atggtattgc cattccggta aaaagtcagg gtcacttcgt cgcccggtct tttgctcccg
     1141 attttttcat ggacatcgtt gttggaactg atggcgatct ggtccacgcg cagcaagaca
     1201 tcccccgcct ggaggccggc atcttccgct gccgactggg caaacacctt ttccaccagg
     1261 accccggaat ggtccggtaa tttgaggtcc gccactttct ggttgtcgat gtcgtcgttg
     1321 agggtgatcc ccagaaaagc cctttgggat tttccgaatt ccttcaggtc ctggatcact
     1381 tttttgacca ggttgctggg aacggcaaag gaatatccct cgtagttgcc ggtatgcgtg
     1441 agaatggcgg tattgattcc caccagcaaa ccgtgggcgc ccaccagggc tcctccgctg
     1501 ttgcctggat tcaccacggc atcggtctga ataaaggact cgattgaaaa atcacggtct
     1561 tccaggatat ttatatttct ccctttggcg ctgactatgc cagcggtaac cgttgatctc
     1621 agtttttccg ggtttccgat agcgatgacc cagtccccgg tctccacttc atccgaattc
     1681 ccaaaatgca gaaagggcag gttctgggcc tcaattttca gcagcgcaat gtccgtgctc
     1741 ggatcatcgc cgacgacttt ggccgaaaac ttttgtttat tgtttaaagt gacttctata
     1801 ttggcggaaa ggtcttcgct gccggtttca atcacgtggt ggttggtcac gatatatccg
     1861 tccgcggaaa tgatgacccc agaccctgag ctgtggtcca ggtattcata accccagcga
     1921 ttggtcctga tttcggtgga ccggatatac actaccgccg gggtggattt ccgcgcggca
     1981 tctctgaaac tgaccgggtc atcgggtgcg gaattggttt tgttgatgct tgttgtggaa
     2041 agagggtttg attcgacata agggttctca gaagaccagg caggcaggcc cgaacgggaa
     2101 tcggaaaaca ccgcccttgg ttcccttacc agtatacgct ggggaaattg tttactgtaa
     2161 atgaatatcg taaacatgga actggccagt gcagtcacca ggatataaag gaaaggcttc
     2221 ataggagatg tttgactccc gcaagttagg agaaaaaaag ttctggtgaa aatgccgctc
     2281 ccggggaaat cgttagtatt ttattaaacc tgcggaataa aacctaaggg atgatcggca
     2341 atgaccatgt ttaatcatcc caattcatct ggggggagaa ggtcgttgag ggccccctga
     2401 agttcattgg cgtcgtgcca ggctttttgt tgccggcata gaaccaggcc atgctcgtaa
     2461 acttcttttg ccttgtccgg gctttccagc ctttcataaa gttttccgag gtgataatac
     2521 gcgcccgtat agcggggatc ggcctgtagc aattgttcat aggtctctaa actcgccgaa
     2581 aggtttcctt ccgtttcata ttctttggcc agggcaaaca acaggaaggg ggaagcggga
     2641 tctttttcca gaaaacgcag gagggtttct tttctatcca taattccagg gtgaaaggta
     2701 ctgttttagg caataccggc cggtccattc cgaaggagcc ccctcctgat aattgattaa
     2761 ttttgggcca tgaaaatatt ggtatgtatc tccgtcaccc cggataccac cagccgcgtc
     2821 agttttaaaa atgacaaccg ggaatacaac ccggatggcg tccagtttat ccttaaccct
     2881 tatgatgaat ggtatgccct ggtgaaagcc cttgaaatca gggaaaaatc cggaggttcc
     2941 gtcacggtga tccatgcagg ccccgtgcag catgaaccca tgatccgaaa agccctggcc
     3001 attggagccg acgaggccat ccgcattgac gtcaggccgg attcgagtca tgccgcggcc
     3061 ctccagattg cagccgccgt gaaagatcag gctttcgatc tgatcctcac gggtaaggaa
     3121 acgattgatt accagggggc ggaattggga gccatgttgg cagagtggtt ggccctgcca
     3181 tttgtgccct ttgcaagcaa attggacctg gaagggtcca gggccatcat cgaacgggat
     3241 atcgaaggcg gggtgcaggt cctggaagtc caattgcccg cggtggtcag tggcgccaaa
     3301 ggcctggccg aacagcgtat cccgaatatg aaaggaatca tggccgccaa atccaagcct
     3361 ctttcggtca gggacggcgt tcccggagag gatttgatcc tgcccgcggg cttcaccctt
     3421 ccccctccaa aacaaggggt ccgaatggtg gcgccggaag acatggacca attggtcagg
     3481 atactccacg aggaagcccg ggtcctttaa actttaaatg aaacgaacat gtcggtattg
     3541 gtatttatag aaaccttcca gggcggtttt aaaaagcctg cccttgaagc cgtgagttat
     3601 ggtcatgccc tcagcaggtt gatgcaaggt caatgccagg tcctggtata tggaaagacc
     3661 gaccagccgg gtctgccggg aaggcacggg gcagattcgg tcctttacct ggatgacgag
     3721 gatggacttg ccgataccgc ctggctgtcc ggtattatcg cccgcgaagc cgccaagttt
     3781 ggtgcaaagg ccctgatcct ggcgcagaac agcaccagcc gcgggatcct tggccgtctg
     3841 gcggtcagtt tgaaaagcgg ttgtgcttcc ggggtcacgt cactgccgga aatcaaagag
     3901 gaccggatgg aggtgagccg gtcggccttt gccggaaaag cttcagcccg cttgtccctg
     3961 ggcgggccat gtaaagtatt gagcattgca tccaatgcgg tccttatgaa aaccgatgga
     4021 caggatgctc ccgttgaagt ccttacccga cctgaaccct cagggaaagt taaagtattg
     4081 gaaaccaaac ggatcagcgg agagatcccc cttgccgaag cggagcgggt ggtttccgct
     4141 ggccgtggga tgaaagaacc gggcaattgg ggcattatcg aggatctggc ccatgaattg
     4201 ggggcggcca cggcctgttc gaggcctgtg gcggattccg gctggcggcc acaccacgaa
     4261 catgtcgggc agaccgggct ggcgatcaaa cccaatctct acatcgccat cggcatatcc
     4321 ggcgccattc agcacctggc cggggtgaat aattccaagg tgatcgtggt catcaataag
     4381 gatccggagg cccctttctt caaggcagcg gattacggcg tctgcggaga tttgtttgag
     4441 gtggtacccc gcctcaccgc cgcagtgaag cggtacaaag aacaacatca ttgatggata
     4501 tcatcaaatc ctacagcagc ttcgggcaat tttccgcata tttgcactag agaataatga
     4561 aaaaaattga actggaaata gtaacccttt cccacagcat ttcacaatct cacaactatg
     4621 ccgtggtatt gggcgaactt gaaggaaacc ggaaactgcc tatcgtcatt gggagttttg
     4681 aagcccaggc cattgccgtg gccatggaaa aaatgaaccc caaccggccg ctcacccacg
     4741 atctgtttaa aaacacccta gatacttttg atattgagat caaggaagta gtgatcaata
     4801 acctgctcga cggcattttt tacgcccgtc tcatctgcaa ccggaatggg gaaaccattg
     4861 aaatcgactc ccgcacttcc gatgccattg ccatggccgt aagggccaac tgtccgatct
     4921 atacctttga ttttatcctt gaatccgccg gggtcgtatt ggaagaaacc gaagaaaaac
     4981 cggtcgcggg ggttaagaaa gaaaaaacgg gcggaacctt caatcaatat tccatcgacc
     5041 aactgacaaa aatgctggaa gaagtgctgg ccgatgaaga ctatgagaaa gccgccaaga
     5101 tccgcgatga gatcaacaaa cggaaggaga ccaatcagta agttttattt cccccgcttt
     5161 ctcaggacta cccggttgag gataaattga ttttttgtat ctgaggcctt gaagtgaaag
     5221 gagatcgtct gatgaaggcg gtcgacccga agttgggtga tctcctgttg ctcgattctt
     5281 tttgtttcca ccgagcctgc ttgcacccaa tcgcttttcc ggctttgcct ttgccagatt
     5341 gaaaaatcac aggcgcctgg gccggtgata aaatccagat ccaggctgta ctctccttcc
     5401 gggatctcgg ccagggacat ccggatgacg gaccccggtt ttatttcaaa agagtaggtc
     5461 agtccgccgg tcgggtagga ccaactggtt ttcaaccgga tatcgccgtc gagcgaaaaa
     5521 gaggcgatct ggggataaag tacgaaggta tccggccggt ggacccgggt gctcccggct
     5581 tccggcgcag gctgcggaat tccggcctga tcactgtaat aataggtgac ggaagtatag
     5641 gctgcaggga tattgttttg gaccgggccg tgctcaatgg tatgatgaaa ggatgtttca
     5701 taggatattt tatccgcaag gaagaaccgg tatccgcccg tccggcaata gggcagggaa
     5761 taatccaggg aaccggacaa aggtaaactg taggttccat cccagcggtc caggagggca
     5821 taccagcccc cgttgaaata atcctccgaa cccgtgccat ggatgcgggt ttccccatcg
     5881 atcctgcaga cgtcgtcccc ttcaaaaaac aaggtcatcc ccggtctgag gccctgacat
     5941 tggagggtgg tgccaataaa atggccttta ccgtaaacct cgagcaggtg gtgaggcctg
     6001 ccttccggta atccggtatt ggaggagtaa taagaataaa acttgccctc cagggcggga
     6061 tttctttcaa ccggttgaaa ccagagtgtg gattcgatcc ggaccggctg ttgcacaatg
     6121 cccggcctgc gccggtagat cagttcgatc ctggccgaac ggtcaaaggg catagggaaa
     6181 aaacagtagt ttttatctcc ggaggatccc agcaacaggc ttttcatgga tacccgccca
     6241 aaggcaaatc caaaaaaatc cgccacgggg caatagaccg cgggccggtt ttccccgtcc
     6301 caggtaatac gtatatccag gtctttgtga agtccttcaa aagcggagga agggctgagt
     6361 tcaaaaccgg caatgcgtcc gccccgggcc aaatcaaata aggtggtttg cccgcccggc
     6421 tggaggctga ctgccttttg gtaggtggtc accgggtgat ttccggaaaa aagcgctatc
     6481 gtgggatcgg ccgaccaggc cttttccagt tgatccaaaa tctttttctc ctccgggttt
     6541 aaccggggat caaaggattt tatggtccgg gattcgggat acagccggta ttgaatctgg
     6601 tggaattggg tccttttgcc tttgtaaacg atcctgcaat gttttgaaaa gggaatgggc
     6661 agataggaat aaaaaccacc cagttgattg ccgcagagtg gagcgacaaa aggataaacc
     6721 tcgcctgaaa acaggtcccg gtaacgggtg gtaaaagcgg ggcggagggt atcatcaata
     6781 aaaaaatcaa ggctgtcgtc agaaggggta ggggtccaga tgcgctggat caccccgggc
     6841 ccctttaaat caaagaggac caggctgctg tccggatttc tgcggaggta ggaatatttt
     6901 ccgctgaagc cgtcgtcgtt cccgccggtt gtatcgtaag aggacacctg ggcgcaatac
     6961 gtatccggct gataagaagg taaccggtga aactccagga agtccttcaa taaaccggcc
     7021 aggcccaaag actccggaac ctgaccgaca gacctgggtc cgaataatat aagggatggg
     7081 agcaaaatgc ttttcaagat ggattgaaag cgataggaat ctggcatagc cgttattttc
     7141 gtccaattta tggacaggtg cgcggatttt aagagggaat gaccaacttc ttgtcctgga
     7201 ggggcaggaa ggccgagaga ataagcgcaa tcaaaaccac caggccgcag ccgatcaggt
     7261 tgaaccagag aaatccgata tcggtggtat tgtataaaac gatgactgtg atttcagaca
     7321 ggatggctgc ggtaaaaacc gcatggcccc gggtgtactt caggtaaaag gcgcagagga
     7381 ataccccgag gatggttcca taaaaaaggg agccgatgag gttgacaaac tggatcaggt
     7441 tttcaaatag ggtggcgtac atggcgaagc cgatggcaaa caatccccag accaccgtga
     7501 atacccgggt gaggaagatg atcatgagat cgtccagttt cctcctttta aataagcggt
     7561 ggacccagtc gatggacgtg gtgcccgcca gggcggtgat ttccgctgaa gtggaggaca
     7621 tggtggcagc gaaaactaca gccaggagca agccgatcag ccccatgggc agatggttga
     7681 ggacgaaatg gaggaagata aaatcgcggt cattggtctc ggtatccggc agggcctgtt
     7741 tgatgatggc ttttccttcg gtcctgaccg tattttcctg ctgcaggaga ttttggagtt
     7801 gggtcttcat ggccggatct gcgtcagttt cttgcaacag ccgggattgc acttcctgtt
     7861 tgcctttcga aatttctgcc agggcggttt gtttttcctg gtaggctgag gcatggtttg
     7921 agctcaaaat ctttgcttcc acctgctggt tgaaaaacag gggactgccg ttgacctggt
     7981 agaatacaaa aaccatgacg ccagtaagaa ggataaaaaa ttgcatgggc actttcacca
     8041 ggccgttggc gatgaggccc aggcggcttt gggtgatgga ttgcccgccg agataccgct
     8101 ggacctggga ctggtcggta ccgaaataag ccaaagccac aaaggctcct ccgatgatgc
     8161 ccgaccagaa attgtaacgg tccttgaggt caaaattaaa attgatggca ttcatttttc
     8221 cggaccatcc cgcaatctga agagcctgat caaatgaaat tccttttaac ttatatagga
     8281 tgaggaaaaa gataaacagg agccccccca gcatgacgat catttggata ctctgggttt
     8341 tactcaccgc tttcgcgcct ccgctcacag tgtataaaat gacggcgatc ccggtgagga
     8401 ggatgaccag tttgaaattc cagccaaaaa gggcgctcag gatgatggcc ggggcgtata
     8461 aggtgatccc cgccgacaag cccctttgga tcaggaaaag gaaagaggtg agcaggcggg
     8521 tcttgaggtc aaatcttttt tcaaggtatt cataggcggt gaacaccccg gaacggaaaa
     8581 aagccggtat aaacgtgacg cagatgatga gcatggcgat gggtaatccc aggtaaaact
     8641 gtacaaaccg cagcccgtcg ctgtagcctt gtcccggcgt ggagaggaag gtaatgccgc
     8701 tggcctgggt cgccatgacg cccaggcata ccgcccacca gcgtccttcc gtattgcctt
     8761 tgaggaaaga atccttgtcc tgaggccccc gggttttata tatcccataa gcgacgatac
     8821 caaccaggga gaagatcaat atggcccagt caaaataact catgaacgcg ggcaaagata
     8881 agttttgcct tatagaaatc aggcccgttg ccctgttatg atatgcttaa gatttggaaa
     8941 ggaatcaaat tatgcaaaag agcccgggat caggaacaca gcccttttcc gcggcatcgg
     9001 gggaaaggat tgaaagggaa aaactttgta ggaaaacttt agagaataaa gctgtatctt
     9061 cgttgtatat acaatccgtt tttcttatgc gaaccaaagt tcaaaaatgg ggaaacagcc
     9121 ttgccgttcg tatccctaaa tccttcgtcg tcgatgcgca cttaagtccg gattgccttg
     9181 ttgaaatcag gttagaggat gatcacatca tcatcaaacc tgttgcaaaa ccatcctgga
     9241 cgcttgaaga attgctggct ggtatcaata agcgcaacgt ccatcgggaa gtgtatgcag
     9301 gtccggccca aggcaaggaa atctggtgaa tcatggccta tattccggac cgcggggata
     9361 tgatctggat cactttaaat ccacaggcag ggcatgagca gtccgggaga aggccggctt
     9421 tggtcttatc cggcgccgct tacaataaaa aggttgaact ggccattctt tgtcccatta
     9481 ccagccagat taaaggttac ccttttgagg tgttgattcc ggacggattg gaaatcaccg
     9541 gagctatttt agcggatcag gttaaaagcc tggattggaa agccaggaaa gccaccttcg
     9601 caggaaaact gcctgaatcc tccttgttcg aggttatagg aaaactgaaa actttgatcg
     9661 gataaataaa aaattaattg taccgattgg tcaatagtat cagaatcagg atgatggcga
     9721 tcaatacggc aatcatcatt ccataccacc tgttccagtt gtaaatagat ccgctttctt
     9781 tcatggttca ggatttaggg tgttttttca gggccagcag atttgcaaaa agccggtagg
     9841 cgccgggcac accggaaggc aattgccgga aaaacgcgag gccggagtac acataatggc
     9901 catccccgta cttggccacg agcaaacctc catctcgggg gggctcaccc gcgtcattgg
     9961 cggacaggat ggcctcaaaa tgttccttat cccattccgt aggataatac aatcctcttt
    10021 cctggaccca gccgctgaaa tctttttggt tcagtttatt gggcacgttc aacacgggat
    10081 gatccggttt gagaaagcgc atctcggcat cttcttcgga gacccgctcc cttcccagtt
    10141 ttaaagcgta aggtgcaagt tcagggctta ccaggcggaa attggtattg tattgcacga
    10201 ccacggtgcc tccgtttttg acataatcca tcagcaccgg atgcagctct ttgagctttt
    10261 cattggtatt gtaagcacgg atacccagga caatggcgtc atatttcgtc aattgttcgg
    10321 cgctcaggga ttgttcattg gtcagtacgg tcacctggta tccaacctga ctcagcgatg
    10381 aagctacatc gtcaccggct ccattgatgt aggcgatttg ggttcctccc tttttaatat
    10441 ccagcctcag taatcgcgct tcactggtgc gttgcacggt ctgaaaggga atatggtcat
    10501 agttgatcgt gaccagttcc ttgctgtaaa ccttgccatc gatgtcgatt tcagccgtga
    10561 ccttggcctc ctggcttcct gtgggaggat acaggaggaa ggaataagac tgctggtttc
    10621 ccttggtttt gatatccacc gggaggcttg ccggttccgt gcgccagccc gcgggcaggc
    10681 ggagcgccag tttacctttt agggctgaga tccccgattt gacctggatg gtcacttttt
    10741 ttggtgcatc gtcggcgaaa acataaactt tactgtccag gttcacaaaa gccggtgtgg
    10801 ttatttcaaa aggcctgtac aattcgccct ttgcaggatc cacccatttg taaaccatct
    10861 ctctctcaaa ttgaaagggg gtgccatcaa tggtcatatt tacctggacc ttgcattccc
    10921 ggggagtaag gggtaaacct cgcagttttt ggtcggccac ggtatacatc ccgtcttccc
    10981 agggttcggt caaccaatag gggctggtgt ttttggtgtt ttcggggatc gtcacggtac
    11041 ggttgaacct ccatcccata ttcggggcca gaataatggc gcccgccgta tcggcgccct
    11101 ccatgggcaa aaaccggacg gactcgagga tgaccctggc attgctgcga ttgatgattt
    11161 cagcggtaac cactgtcttt tcacccgggg cgacggtatg ggtgttgctg acggcttcca
    11221 taaaaaggcc ggcgcattgg gcgatcaggt tttttacatc ctcggatttt tttgatttcc
    11281 agtaaccgtc cggcagggcc tggatcattt ggtagacttt attgagtttt ggtatggagg
    11341 cggccgggtt gtcgtattgg aaggtccggt ccaggtcctg gatcagtggg atcagggcag
    11401 cgcccccttt cacccggttc caggtaatgt ccattccact gaagggattg tccagggtgg
    11461 tgggtttatc accttttagc aaatccaggt attccatgga tgttccccgg ttgccgaggc
    11521 cacccatgcc ctggcagcga tgcatgcttc ggctttccgc ggcgatttcg ccattggatt
    11581 ttcccagcca ggggaaataa acgcctgcgt ccatgactac catattggat ttgtcgactt
    11641 tatcaaaggc ctcctggctg ccgtacagaa accagatggt attgaaaaac aaacgccggg
    11701 gctgccatgg actgacgtat ttgagctggt ccgggaaaat atctttacgg ccggtgaggt
    11761 caaaggcttc ggtggaaagg attgccgagg tgatgtggtg cccgtggttg gccctttcga
    11821 tattgtcatt aaagcggttg atgatgatgt ccggttgatg ctttcggatc tgccagacaa
    11881 tatcgctgag cacttcctgg cggtcccagg tcttcatggt ttcagcgggg gtcttggaat
    11941 agccgaaatc attggcccgg gtaaagagtt ggtttcctcc gtcgattttc cgggcctcca
    12001 gcagttcgtt ggtccggatc acgcccaaca gatcttcaat ttcggaaccg atcaggtttt
    12061 ggcctccgtc tccccgggtc atggacaggt aatacacatc tgctttgtat tgattggcca
    12121 gcagggagat cagccgcgta ttttcatcgt ccggatgtgc agctacgtaa aaagccgtag
    12181 ccagcacatt cagttttttt aaggattggt ggatttcggc ggaggtgggt tttttaggcg
    12241 actgggcgac ggagaaggca accagaaaaa gagctgggag caaagcagtt aatcttttca
    12301 tgctgatgga ttcggatatg attggccaaa tttataaaaa ttaggtccgc cgggcccgca
    12361 gggatgtatt aaagaattta acagttgtca gctgggagct gggagttagg gcgcttgtgc
    12421 aaactactac acagctttac ctcttcagca cagggaccga cttttaattt gcatgaatga
    12481 atgggcaagg ccccctaact tcttgcttcc agcttctaac tactagcttc taattcccgg
    12541 gagctggggg tagagcgctc ctgcaaacta ccttgcaact tagcctctta agcacaggga
    12601 tcgactttta atttgcagga atgccagagg caatgcctcc ctagcttcta gcttctagct
    12661 tctagcttct aacctctaat tcccaagagc tgtgagccag gggtggaatt tggattctcc
    12721 tttctttcta tctttaacct tcattaaaat tatccagccc gatggcctcc tcaccttcca
    12781 gtcccgattt cagttccctc ctgctcaggg taaacaatta caaacgaatc ctccagaata
    12841 cgattgaata ccggaaagcc tggcctacag agctcaagcc cctgattgaa tcggttctta
    12901 aagaatttct tgctcaatcc ggactgaagg gggaagtgcg ggtgcaggac cggattgaaa
    12961 acctggagtc ggttgtgctg gacctgggca aaagccacag cgggatcagt gaaaacctgg
    13021 acgacacgga catcaagcgg accatgatta aatccaacgg gaccctgatc tatcagcaat
    13081 tattcaacgg gaaaatcatg gtcatggtac tgaatcccca tattgaaggg tacggtgagc
    13141 ccaaggcacc gattaccctg gagatcctca ggcctgaaga attaaaggcc ggatttatgc
    13201 tccggcacct cgagaccttt ctcaaaaaca tcacggaatg ggaggattac gacgatgacc
    13261 tgcccaccaa aaccccgatc gggtttcaac ccgtgggctt ccaggtggat gtccccaacg
    13321 aatgattcac cggggtatac cggggagatc acgcgtcccg gcgggtcctc atgctaagga
    13381 ctcaaccgcg cgcaaatgat gcctgccacg gccgcctgga tcacgccgta taaaccccag
    13441 gacaatacca tgggcaccga aaccgaaaac gcgctgtaga tcatccaggt gcccgggagg
    13501 atggccacca aggcataaac caggcccagt ttaagcccgc gaagccaaaa actgcccgta
    13561 aacatggact taaaattgtt ccagaaccag gccagtacga aactgatgat atagggatgt
    13621 acaaaaaagg tccaggcctt ggagccctcc aggctgaaca tcgggtcata atattcttcg
    13681 gccaggtggg gaaatatttt tacgagcagg aaaagggaaa agtaactgaa taataaaagc
    13741 acggttcctg cggttaatcc ttttaaaatg atttttttca tatctgtagt ttgaaaaggt
    13801 tattggagta aggtcttgcc gggcaaaact tctgcatgca ccaggaggtt gaagtggaag
    13861 gtaatggggt cgtcgacttt gatcatgcca aacaaggcta ccggggggct gatgttgaag
    13921 tcggtcatat tcatggtttt tttccctgta aaccggaact ggagatgggc atcccgggtc
    13981 gtcctgaccg ggatttcata actccgggtc actcctgcga tggtgatgtt tactttagcc
    14041 agggaaggaa gtccttcgtc gctgccggaa aggggaattt cgccgggata catggaaaat
    14101 tgtttgagct caatcccaat atacggatat tgttcggcct tgagggcctt ttgaaggtcc
    14161 cggtccattt tgccgttttt gcagtcaaag tagatgaccg gcaactggag gctggtaccg
    14221 gtgaaatcca tcaattgctg cgggttcagg tccaattgaa atttctgcgg atcgaaggta
    14281 ttgccgcagt cgcatttgaa tttattgaca ttggtggtac cggctaaaaa aactttgctc
    14341 tccggcagga ttctgaacct gccttcgagc aaatcttcgg gggcaaaaac ccttgtaaat
    14401 aaaccggata tcagtaaaaa gaacctcagt atcataacaa aaaaaatggg gtaaggccgt
    14461 taaaacctta ccccgaattt aaaaaaatca gaatcccact actgcttcta ttactgctcc
    14521 attgaactta ccattgtacc tgtaatcggt gcttgggaag tctttgtatt tctggctgac
    14581 gatttcagct ttgagcagga ggtttttggt cgggaaccaa cctgcagcca cggcaaaccg
    14641 gttgatgttc acatccgagg tgatcccgcg gagttccgct ttggcaccga tctgacgaac
    14701 cccaatatac atggactcat tggccaggaa acggtagctg gcttcaacac tgtattggga
    14761 tgaatttctt tttacccatt tgccttcttt tacatcgcca taaacactgc cggaaagggt
    14821 ttcataagaa ccggtgacct caaggccttt gtatttcaaa aagggattga tcatgaaggc
    14881 ggtgatgcgg ttggccatat ccggattaag ccggcctgaa gtgaactggg cagccatcgt
    14941 ggtggcggct ccattggtca gggcaggttc catagccagg aaataatggg atccggtccg
    15001 gtcgcctgcg tacagggtat ttctggaaat gttgggattg gtataaacgg aagcggaaag
    15061 tctgaagcgc aggtcttcaa aattgttgtc ataagccaat tttgcaaaaa tgctgggggt
    15121 ttttttggtt gggaccactc cgctggaatt gttgctggtg gggaaataat ccacgttccc
    15181 gttgatgtag ccggaggtca tgccgaccat ggccataatg cttttggtgg gccaggcgta
    15241 aacttctgca ccgatttcgg tggtgaacgc atcaaagatc aggttctcca caaagggatt
    15301 gaacatggcg tttcctccat ccgtcctcct gaaatgcatg tcgccgaaat tggggacaaa
    15361 atggccgatc ttcaccctga aattatcggt gaaccactgg ggattcccga acatcgggag
    15421 tttatcgatt tggacatatc cgccttttac ccagaactcg gaatggtggc gggaagacat
    15481 gtagttttca agcctgacca tgatcccgtc ttcgatctgg aaatcaaaat tgaggttggc
    15541 catggcgagg ttgaatccgc ggagcagggc agaagttgaa tcttcagctt ttactacgcc
    15601 atacaggtaa ttggcgcttt tggtaggggt gttggagtgt ttgaggtgct ggtaatcctg
    15661 ggtaaatgaa ccaccgatcc gcaatttgaa gccctcatag gccggttgat cgctggattt
    15721 gaccggttca aaaatgttta taccttcctt attccagggg cgggaatact ggatgtcgga
    15781 ctgactgaac aggcttgtac tgattaggag cagggcaagc agcagtaatg tggatgaatt
    15841 tttcatatgg taagttattt tatgtttgaa taatagattt ttcattttac gagtttgact
    15901 tcatattcaa tggtgatctc atcgccggtg gtcatggtgc cgagcagggc ggtcgggggt
    15961 ttgatgccga agtcggtcat tttcattttt ttactgcccg atatgctgaa ctccccattg
    16021 ccgagggatt tgaccgtact ggtcaggtcg atcgccttgg tgctgcacgc gatggtcaga
    16081 gaaccttttg aattgaggtt cccgctgcca tccgggctgg gagcggacaa taaaaaactg
    16141 atgttggcac attcctcgga tttcagggca tcataagtct tgttgtccat gatcttccct
    16201 ttcggacttt tgatgctttt aacgggtatg tcaaaccggg cttgattcag gcttttcagt
    16261 tgttctccgt caacagtcag agtagcggtg ccactcaact ggcctaccgt ggactcccat
    16321 ttgtgtacat tcgaggtacc ttttataatc actttggtaa gcgatatgcg gtaactctcc
    16381 tggctgcaaa caaaaaccgg gaggaggaga attagtagga gattcgtgat tttctggatt
    16441 ttcatttttt aaatttacca taaaggaaat atcattttga aaaaccgatt ttgacctggg
    16501 tcacgcagaa ggataatctt gggcaggact ttggaatacc atgaattcat tcgagtagtt
    16561 tggatctaaa ccattgatca tcagcaccaa acgaattaac gggaaaaaat ttctttttga
    16621 ggcatcgggc aatctatgat cgtcgtcagg ataatcgatg accttgattg ggttgcgagg
    16681 gggctaattg gtcggttttc ggagcgggcg actagtttgc cggggtattt taacgggagc
    16741 ggccgggatt tggggggccc tccctggggt tccgtcccac cggtttccgg tataaataaa
    16801 tattgtacaa gtcgggatta aatcgtattt ttgccgaccg ttttcggctt cgtagctcaa
    16861 ctggatagag catctgacta cggatcagaa ggttgaaggt tcgaatcctt ccgaagtcac
    16921 tgaaggaaat aattaagaat caatagttaa taattaataa ataaatcaac tatgtctaga
    16981 acctgcgacc tcaccggcaa aagacccaga aagggcaatc atgtgtcgca ttccaaccgc
    17041 aaaaccaaga ggcattttta tcccaacctt caggataagc ggttttgggt accggaactg
    17101 aataagtgga tttcgctccg tgtttcggtt tccgcattgc gcacgatcga caaggtcggg
    17161 atctaccagt ttattaaaag tcaaaaagca aaaggtctgg cacagggtgt gcggatctga
    17221 tcctttaaaa acagacaatt atggcaaaga aaagcaaagg caaccgtcag cagatcatcc
    17281 tggaatgcac ggagcataaa aacagcgggc ttccgggcac ttcccggtac atcacccaaa
    17341 aaaacaagaa aaacactcct gaccggcttg aaattaaaaa atacaatccg atcatgaaga
    17401 agtataccat tcacaaagaa attaaataac catggctaaa gtcagtaaaa acgcaaaagt
    17461 ggcacagcgt gccgccaatg cagggtcggg taaagacttt gtcaaagtca tcaagagcgt
    17521 aaaggatccg gtcaccggaa aatattcgtt caaggaaatg atcgtgcaca aagacaaagt
    17581 gaaagagttt tttgccaaca aataaattct tcacctgatt aaagaaaaga gaggcttttg
    17641 gtaaacagga gccttttttt atttcttatc catccatcat gagttttttc aacaagtttt
    17701 tcacgcggga gaaaaaggag gacctcaacc agggcctgga aaaaaccaaa acctcctttt
    17761 ttaccaaact aaccaaggcc atcgccggca aggacaaggt cgatgaggaa gtcctggatg
    17821 aactggaaaa tgtactgatc acctcggacg tcggactgcc gaccaccgtc aagatcatcg
    17881 aacggatcga agcccgggtc gcccgggaca aatttctcaa taccgccgaa ctcaacggta
    17941 tcctccgcga cgaaatcgta aacctcctga gcgaaaacaa caccgttgac ctggaagact
    18001 atgaccttcc agcccagccc cgtccccagg tgatgctggt ggtgggcgtc aacggcgtag
    18061 gcaaaacgac gaccattgga aaaatcgctt tccagcatac gatccgcgat aaaaaagtag
    18121 tcctgggggc gggcgatacc ttccgtgccg cagcagtcga tcaactcaaa atctggtcgg
    18181 aacgggccgg ctgcctcttt tttagcaagg gcatgaatgt ggacccggcc gccgtggcct
    18241 acgaaacggt tgattttgcc ctcagccatc agtgcgacct ggcgatcatc gacaccgccg
    18301 gccgcctcca taccaaactc aacctgatga acgagctcgg caagatcaaa cgctccatat
    18361 ccaaaagact ggaaggggcg ccccacgaag tcctgctggt cctcgatgcc accacaggcc
    18421 aaaacgccat cgaacaggct aaacattttt cggaagccac cgacgtcacc gccatcgccc
    18481 tcaccaaact cgacggtacc gccaagggag gcattgtact cgccatttcc gaccagttca
    18541 agatccccat taaatatatc ggagtcgggg aaggcctgga taaactccag atcttcaata
    18601 agaaggcctt tgtggattcg ttgttcgagt aaggagtggc gagaagcgag ataggaaagt
    18661 gacaaaatta aatttaaaag taatttacaa aactcaccaa ccctttttta agatactttt
    18721 ttttatgact aatctagact tgaattgaat tatatttaaa aaccttgaaa ctcattcctg
    18781 atcggacaac aataaagtat aatcctttag ccaaattaaa ttctcttaaa ttaatatttc
    18841 tataaaagta tttttcagcg aataattttc catcaatggt ataaacatat atagttgatt
    18901 tattatcatc ctgtgtagaa ataatatctc cataaatatc tgcttttaga cttccttttt
    18961 gcaaatattt taatggtaca gttttttgat tactgactat caattgacca tttcctttta
    19021 ttgaatggtc aattaaaatg taataatcat cattttctat tgtatttaag accatgtaaa
    19081 tatttttgtt gaagacattt atttcagaac aggttttttc ctttaaacat gaaatattgc
    19141 cacttaaaat tgcgacttgc gatggagcgt ttgaagttac tctgaatgaa taagattttc
    19201 cgttaccttt aaaataataa aaataaggat aatccaaatt tttactcgag agccaacaag
    19261 atggcaaagg gagattggat aaataatggg atgtcagatc tgttttaaaa ttaaagataa
    19321 aggaatcttt ttctggtagc aatatattct gagaatatat cggagcatca taaattctat
    19381 ttgaattatt gcaagaataa ttatataaat ttttatgata taatccaatg gcgccatgat
    19441 gtccgaatgt aataattctg tcaaaactat aataattatg attagcggta catcctccat
    19501 caattgacag tccatcaccc atccagaaag tatttgtatc taatactgca aatatcccag
    19561 gagatgtttc ttgtgccatg agatatttat tgaatattaa cttatcaaat tgttctcctc
    19621 tataaaggcc attagaaaca tatatgaata attgattgtt agaaaatgtc aaaaaatgaa
    19681 tatttgatct aaaatttggg tatttaatta atgtgttgta aggctcctct ttcgaataat
    19741 atagctcatt ttgtgaagct attattaatg tgtttgaaat ataacatatg ccgaccaatc
    19801 tatctaccgt gcttgaaaat attttattct tatatttttc taatttatca tccttaaaaa
    19861 ttagtattga tctatccaat cctataaaga atttattttc attgtgaaaa tatatactcc
    19921 taatgctttc agaaattagt gtatttgaat atactaagct ccaatttaag ccaccatccg
    19981 tagtttttat aataaaaaat ccattaaaat aatttgctat gacataacca gttttagcat
    20041 ttataaaata aattcttgaa gttgaaaata tttcaaatgg taacttataa atatttgttg
    20101 aagatgaatt ttggtattga taaatggaat tattacttag aataaatcca aaatctggtt
    20161 gtgtaaatga gatatcaata atatttattt tatcgtcact aagaaatagt ttccaacatg
    20221 gaaatgtatg attagtgcta aatggatttc caataaaaga ataacatgat ggatattttg
    20281 tattgcatga gtttccttgc gaaaatacag gtccaatggc ggaaattgac atgtacagaa
    20341 taaacgaatt taaaaatttt aaactagaag tgaatttcat ttcatattga tttatattaa
    20401 aatatataaa aaatgttaga aattaaatta tttaatttat gacttaactg agataatata
    20461 taatcgtcaa tcattaagtt gtagttaata atttataggt ttttataaag aaatttaaaa
    20521 gtacaggaac tgaaattatc atataatatt ccgtaataga ttccttcgga taaatttggt
    20581 atagttaaag taatgagatt ctgattggac catagatttg catttagaat agtttttcca
    20641 aggccatcaa taatctttat aaagtattta ttagtactgt tgacatttat ttttaaattt
    20701 aaaatagaat ttgttggatt tggataaatt aaatttattt taagttttga agaatttaaa
    20761 gtttgacaaa agtatgagtt taatgtatct aatgctctgg caatattgag gcggcctccg
    20821 ctcacggtct tgccggagag gtcactgttt ttgtctaccg aagccaggag ggctcttttc
    20881 accaggcggg ctgcgtcggc aggattggcc ttctgataat cggcccattc ccggcaggga
    20941 taactgtaca aaaggcctac cgcccctgac acgacggggg tggccgccga cgtgccggaa
    21001 aatgaaatat aggcattgat attggccgat tgggtcgaat agatgccttc cccgggggca
    21061 cccaaatcaa tatggttgag cccgaatccg gcgttataga tccgcctgtt gtctttcccg
    21121 gtattggtca cggcgatcag gaaatcactg ggacaggtgg agggcatatc cccttcctca
    21181 tccacatcag catatttatt ggtcgtggct ccgcaattga caatgccttc caggcccatc
    21241 ttatcgtaca tgccgcacca gatggggtaa tccgcggctt tggcaaagga aatgcctccg
    21301 ctgtaattga tggccacaac aaaagcccct tccttgcctc ccgaacggtt ataccggatc
    21361 ctttggtcaa gcatgtaatt gaacatctcg atcagttccc cgatattatt ggtgccaaaa
    21421 gtggcgatca tcagtttgtt gttccacatc atgccggtga cccctttgct gttgttggat
    21481 tcggccccga tcaggccggc gacgctggtg ccgtggttgg aaagcccgtc gttgatattg
    21541 tccgcgggat gtttttcatt tttatacttc aggctcaggc cgtaaacatc atccacataa
    21601 ccgttgttgt cgtcatcctt accgttgccg ggaatttccg cgtggttttg ccagatccgg
    21661 tttttgagat cttcgtggga ggtgaggaag ccgtggtcga tgaccccgat gacgatggta
    21721 tcgttgttga aattaaggcc gcccttggtg acatcccagg ctttggaagc cgagatattg
    21781 tccaggtgcc attgttggtt gatcagggga tcattggggg tcgaccgggt ctcgagggcc
    21841 aggtttaact cggcaaatcg gacggccgga ttggccagga ggtctttttt gtttaatgga
    21901 gcacctgcct tcagcgtata gatattaaaa tgaggggcga tgttttgggt tttgatgtcc
    21961 tgcctggatc tcagggggga tttggaccag ccggaatgaa ggtcggaatc ggattttcca
    22021 tcccataact ggatgatcca atcctgctgt gcacggacct ggaagcaggc caacccaagg
    22081 aatagaagga gggtactgcg gtatttattc atgggcgatt tttagaataa cgaaaatccg
    22141 cttttcaagg atacaaagat aggatgaatt ttggaggccg tacgattcca tgatgtaaac
    22201 cgcagggggc gcggtgttcg caggggaggc gcaaaggcaa tcattcacct gtatcctctg
    22261 cggatcctct gcgttcacat cggctctgcg gttaatagta actgttattt aaccgcgaag
    22321 ggcgctaaga gcgcgaagat ttcgcagagt tgggagttct ttctttgcgg attttcttag
    22381 cggccatagc ggcctttgcg gttaaaattt tgctttttcc tgcaataggc aaatcacccg
    22441 atctttgtca ggatttgggt ttcttaaaat ctttttcccc aactttaagc gcgaccttca
    22501 ggtgattttc gacaggcggg atcgggcaat tgtatccatc gctgtaagcg caccagggat
    22561 tgtagacccg gttgaggtcc aggataagcc gtccgtcttg gatggcggtg gcctggaggt
    22621 ccaggtagcg gcctcccgca taggtttttt tgcccgtcgt gagatctttg agcgggataa
    22681 ataaatggtt ctggtagagg ggagatacca aatgcaggtt tttatatgca gtgagcctga
    22741 gctttttacc tttccactga aaatgcagga cggcatgacg cctgaatgtt ttttcctgcc
    22801 cgctgtacgt gggcatttga aaagagggct ggtcctgcag cagttccact tcgcaaaata
    22861 ccttgttgga ttctctcacc gggtaatagc cgaggtaaac gctgtcgccg ggttttaccg
    22921 tcgtcctcgg atcggaaaaa aggccggccc agttcttgga tcggtatagt ctgatttctt
    22981 ccttgtagga aaggatttgg ccgtacatcg gcaaaaaaag caggattagg aggaccgcaa
    23041 aaatccctct cattgggctg gctgatttac caaaatcagg cggtccccac gcagggccat
    23101 ccggctcacg gattccaggc cgaagtattt cagatcggcg atttcaaccc agtttttttc
    23161 cgtttccggg tgcatgaaat acagtttgga acccttgcac atgaaaaaat gaccttcggc
    23221 ggagagggca aaatcttcac agcccggcaa ggtctcgctg aggatcaccg atttttgcat
    23281 caatggatcg tattccttga tgtaccagta cgattccgaa attttatgta cataaaccag
    23341 atggccggcg gcgttgcggg tcatgcagcg gccgacgtcg gaggtaaaca gaaggggatc
    23401 tttcgactcc aggttgacca gggccatttg gtggggttta ccgaccagga agagcgccac
    23461 gttctgggta tcgagccagg catgatagcc gacgttttta atgtccggca aggggctgat
    23521 ggctttgcct tttggcttcc gttcataacg gtaaagcctt tgaagacccg tggcctggtc
    23581 atccacccgc acacacgaga tgtattttcc atccggggtg agggtagggg aatattcgct
    23641 gaggctcgtc cgggtcaggg gctggagggt gttcaggccc aggttcagtt tgaagatatc
    23701 ggtttgcccg gctcctttct cctggacggt gatcaggatt tcgtaatcgt tgataaaata
    23761 aggttgattg ttgtatcctc cttcattaaa agcgctgagg taccggggtg aatgcaccac
    23821 aaattttccc gtttctccct tttgaatccg gaattcatac aattgggtcg cggggacctg
    23881 ggcttccagc cggctgagca ggaaggcaaa taaaagcggc caccggcgga taaacattat
    23941 tttttattgg ctgaagtgaa caatctgttc cagtttattc cattggcaag tttaccgttt
    24001 tttgtcgaaa accagataaa caggggtttg cccacgatat gatcggcggg tacataaccc
    24061 caggcccggg aatcttcgga attgtgccgg ttatcgccca tggcccagta atagtcctgc
    24121 tcaaaggtat agctggacgc ttccgcaccg ttgatgtaga tcttgccgtc cctcacttcg
    24181 agtttgtttt tttcataaac gctgatgacc cggtcgtaca ggggaaggtt ttccagggtc
    24241 aaagaggtgg tggtgccggc tttggggata taaatgggcc catagtcgtc catggtccag
    24301 cccagggaca gaggggtatc ataggggaaa agcggcctgg cttcctgggg taccggctgg
    24361 atgatgatcg aggaatccat ggcctggatt ttggcccttt gggtgctgtc gaggaaatag
    24421 acgcccatct gaggcatggc ttccgaaagg ttgatgccca gttcatccag ttttctttgg
    24481 ttgagctggt tgccgggcga caagacccgg tacataaact gcatatgttc cgggttgtag
    24541 gccggctggt tgttgatata cagttggcgg tttataattt tcaggctgtc cccgggcaag
    24601 gcgacacagc gcttgatata atggtctttt ttatcgacgg gccgggtgac cagttttgcg
    24661 tttcgtaccg cctggcgcac ataaggttcc ctgcggtact ggtcggcggt gaaagcccgg
    24721 tccggggcca ggtaaacgct atccccaacc ggccagttga acacgaaggg ctcatatctt
    24781 ttgacttcct gcaatttagg cagccgtttg tatttaaaac tcggttgctt caggtaagat
    24841 tcggtgtcca ggaaagggat gcggttgtgc aggaggggga ccataacaaa ggtcatgggg
    24901 aggcggatcc cgtaatgcgc cttgctcacg aagaggaaat cgcccacctt aaggctgcct
    24961 tccatggacg aggtggggat catataggct tcgatcagga acatccggat gaaggcggcg
    25021 gcaaaaacgg cgaagatgat ggattcggcc cattcgcgga gcgcgccttt tttatacgga
    25081 ttggaggcct ggagtttgtt tacggttttt tggtcttttt tgtcctgtgc ttccttgagt
    25141 ttagcataaa attcgtgctc cttcatgagg atgggaccct ggtatttttc atttttagcc
    25201 aaacccaggt agagggaata aaacggggcg aagactacgg ccagcacgct ctggaaaaag
    25261 gaatgttttc cgaaggatct tgccaggtcg atgatcaggg cggcgaaaat gaaaatattg
    25321 acgatgggga aaagcatcca ccaggcatgt gattttttac gtcccaccag ggccgacatt
    25381 tccgccaggt tgagcccggg gaccaggcct ttccaggctt ccactcccgc cttggggaat
    25441 acgaagtaca tgcctacact gtatatgata aaacctataa gcaggaataa aaacataagg
    25501 accttgtttt gtaagagagg gcaaatataa ggcttatgtc ccgcgtagcc taaggcatta
    25561 gacgaaggac gggcgggctt cgatcaggat agagcaagaa gcagaattta aagaagcccg
    25621 gaaggcccgg ccggaacaac aggtcaggat tcatgccgtt tatttagaat aaaatggaaa
    25681 actttttcag aaacagaatt tggacggccc gctgaggtga aatgcttact ttacagcctg
    25741 tttacctaaa cctgaatttc aaagtggttc tcgtagtcga tctggacggt acctatttga
    25801 agaatgattt ttttgcagaa cggttttaca agaggctgat cgagaatcct ttcttcgtgg
    25861 tctatcactt gttcacgggg ggaatccttc aattaaagaa cctattgctg gataagggga
    25921 gggcagagta tccattggat cctttgatta attctgaagt ggaaaagttc attctggcca
    25981 atcgcaggaa ttacgaacgg gtggtcctcg tttctgcaag ccctgatttt tttgtaaagc
    26041 aagtgctgtc ggaaaatgaa atcttcgatg cggttcatgg gagtgtggag gaaaacttaa
    26101 aaggggaaaa aaagctggaa tttatcagaa atcgtttcgg ggaggatttt cattacatcg
    26161 ggaaccacgg ggtggatatt ccaatactcc gggcggcaaa aaaaggattc atcgtaaagg
    26221 aaaaccgcat ccatgagtac ccttagattt tcgatactcc gcttgctgcg gcccttgcac
    26281 tgggtgaaaa acggcctgat ttttcttcct atgcttttag ccggatcgat tacgcgggtt
    26341 cctgttttgg tggatgcggt gattggtttt ctctcttttt ccctcatggc ttcggccgga
    26401 tatattctca atgacatagt cgatgtggat cgcgatcgcc ggcacccgta caagtccaat
    26461 cgcccgcttg ccgccgcctc cctttccacc gggagggccc tgggggtcgc cgttttgttg
    26521 ctcctgcttt ccgtatccct ttcctggccc cttggaatgc gggccgtggg gtggttgatg
    26581 gtttatttta cactgaattg gctttactcc gcgtatttaa agccggtccg ctttttcgat
    26641 atcgttattt taagttcctt ttacctgatc cgtttattct acgggtcggc catcggcgat
    26701 gcggaactga cgggatggtt tgtggtggcc agcctgtttt cgttcatctc cctttccgca
    26761 aacaagcggt acctcgaatg tgcgatcagc aaacacggaa cccttgcggg caggggatat
    26821 tcaaaggatg acgctccgat gcttcagacc ttgtccgtcg cttttgcttt gggcaccctg
    26881 gtcttgttga acctgcacgc cttttttgtt ttaagcatcc gcgaacccgt cgtgatagcg
    26941 ctgatcaatt tattcgccgc cgggatgatg ttttcctatt tcggaaataa caaaagcaag
    27001 tcggatgatc cggtggcccg cattttgagc aacccttttt tactggccat ggccctgatg
    27061 ttgatgtcga tctatgtttt tgaattgatg cagcagcgat gaaaacagaa cgtatcaacc
    27121 tgttcaactg gggcgggtat ccccatcaca actgcaggat ctatcacccc gaaacggtgg
    27181 aggaaataca ggaactcgtc agaagcaaac acagcttcat cgcccggggt aacgggcgct
    27241 gttacggcga ctccgcctta tccaaccggg tgatttcaac ccttgcttta aatcgaattc
    27301 ttgattttga ccccatctcc ggtttaatag tttgtgaagc aggggtgctg ctcgataccg
    27361 tcttgaaaaa gatcgtgccg gcaggttttt ttcttccggt atctcccgga actaagttca
    27421 tatccatcgg tggggctttt gcgtccgatg tccatggaaa gaatcagcag gtggacggag
    27481 tattcagcga ccatgtatcc tatattaaat tggttgagcc gggcggggaa ttggttcacc
    27541 ttaccccttc cgatgatttg ttccggcaga cggccggggg catggggctg accggcatca
    27601 ttgtggaggt agcgcttcgc ctgaggcgca tagaatctca gatgatcagc cagcgttcta
    27661 taaaagcgaa aaatctggcg gaagtgtttg atctgtttga acagaattcc ggttccacct
    27721 attccgtagc gtggatcgat ggtatggcgc gggggtataa attgggccgt tcgatcttgt
    27781 tgctcgggga acatgccgct cagacggatg ttgcaaacct taaaacaagg gagcttcacg
    27841 ttaattctaa actaaacctt cccttttatt tcccatcctg ggtactaaac cctttattcc
    27901 taaaggtttt taactgggct tatttcaccc ggccggggtc ttccggtaat gggatcattc
    27961 attacgattc atttttttat ccgttggata agattcggca ttggaaccgg atctatggga
    28021 aaaagggatt tgtccagtac cagtttgtat tgcctggggc tgttgcttat gaaggaataa
    28081 aacatgtttt ggaagtcttg tccattaacc aactttggcc ttatttggcg gtcctgaaac
    28141 gattcggaac ctctcatgaa ggccgatatt tacattttcc gatggaagga tacaccatgt
    28201 ccctggacct gaagatcagc caaaggcttt gggcggttct gaaccaactg gacgaggtgg
    28261 tcagttcgct cggcggtaag acttatctgg caaaggacgc gagattgggc ggtaaaaatt
    28321 tcagaaaaca atatccggtg atgccttcga tacccccgaa tcctgttttt atctcgcatc
    28381 agcaggttcg atttcagcaa atgaaaaaac acgttttcct gattcttggc gccaacagcg
    28441 acatagcaaa acaaacggcg ctgctctatc tcaaaaaata tccggaggga tatttgattt
    28501 tagcctcgag ggatgtacaa ggacttcgcg catttattga ggaatccggg atcgggggct
    28561 gcagcgaagc cgtgccgctg gacgtcgaga attttgccgg ccacgcttta ttttattcgg
    28621 ccctgagcgc caaacccaat tgggtgatgt atgccgcagg ggtgctctat accaacgacg
    28681 agtgtatggg cgatgcccca aaaagtgcga gaaatacgag tgtgaatttt tccgcgccgg
    28741 taaatttgtt gaatatcgca gtggatgata acaacccggg acttcaacgg attattggaa
    28801 tgagttccat tgccggcctg agggggcgga agtcaaattt tcaatatgga agtgcaaagg
    28861 ctggatttca tcagtatctc ttcggcttgc gtcaggacct gaaagacagg ggagtggtgg
    28921 tacaagccat tacccccggc agggtgaata caaaaatgac tgcacatctg cccgttcaca
    28981 agagggcaaa caccccggaa gaagttgctt cggcaattct gcagaacacc acaacctttg
    29041 aggtatatcc gaacctgttc tggcgattga ttggaatggt cgtgaaatac gccccggagg
    29101 ttctgatccg gaaactttag aaagggctga tttgaaacca atggttaatt tagcaaatgt
    29161 gcaattttcg accaaagacc tattccgcct ccggggcaat gatgtggttg cccttattgc
    29221 tgctgtcgtc accggccttt ctccaaagcc aggagtacac gaaaatcctg ctgaatgaat
    29281 ttaatccgaa atccatttac aagatcccgg taagccgggt ggagaaagcc cgttacccag
    29341 ccatagattt tcattcccac gattatccga agtcggacca ggaggtggat gcctgggtaa
    29401 aaaccatgga tgcctgcaat atcgtcaagt ccatcgtatt gtcctatacg accggccccg
    29461 cttttgatgc ggtaatcaaa aagtacagca ggtacccgga ccgcttcgaa gtctggtgcg
    29521 gctttgacta taccggaatg aacgaaccgg gatggtcgga gcgggccatc agggaactcg
    29581 aacgatgcca tgcccttggc gcccggggtg tcggtgaact gggtgacaaa ggcctgggtg
    29641 aatattattc caaacccact ccgggcatcg gcatgcacgt ggacgatccc cggctgcggc
    29701 ccctactcaa acggtgcggg gaactgaaca tgccggtgag catccacgtt gcggaagatg
    29761 cctggatgta tgagccggcg gacgcctcca acgacggcct gatgaatgcg gccaaatggc
    29821 atgtggatat gaatgtcccg ggcaaactgg gacacgatgc cctgatccag actcttgaaa
    29881 atgcagtgcg ggaccatccg ggcaccctct ttatcgcctg ccacctggcc aactcctgcg
    29941 ccgacctggg ccgcattgcc ggcatgcttg accggtaccc aaacctctac acggagatgg
    30001 ccgcccgatt cggggaaatc gcgcccatcc ccagacagac caaggcattt atggaaaaat
    30061 acagccgccg gatcctttat ggcaccgata tgggaacctc tgaaaacatg taccggacga
    30121 ccttccggat cctggagacc gcggatgaac atttttatga aaaggaccgg tttaattatc
    30181 actggccgct gcatggactg gatctttcgg ataaaactct gaagaggatc tatgcactca
    30241 acgggaaaag aatcttaaaa agaaggtaat gccacttctt ttcgctgtgg gacctgtact
    30301 tcatttttaa tttataaccg cagagtgcgc gaagatcgca gagggggtgc agagggatgc
    30361 ataaagacgg tctggttttt agtgtacttc gtgtcgtggc gggacctttg cttcagttaa
    30421 tttataaccg cagagggcgc gaagatcgca gagggggcgc agagagaaat taaagacggg
    30481 gctcctgccg gcaggcttta cccttatctc ataaaaacta tattcctctt atccgccacc
    30541 caggtaattt ctgttacccg gagattcctt tcaccattgc tattccgctg gcgcaacggc
    30601 ctgtttggcc cggaggttca ggatatcccg gtcaaacaaa tacaggccgc ctttatcatc
    30661 gcccaccagg gtcagtttgt cattcagatt gcgggcgacc cgttcttcct ccatttgttc
    30721 attgacgtac cattggagga aattatgggt ggcataatct ttttcggcca gggacagatg
    30781 gaccagttcg ttgatgctgc tgctcacttt gagttcgtgt tgcaggaatt catcgaacac
    30841 atgtttgatg gatttaaagg ataccatggg ctgttcgatc gcgggcacca gggcctggcc
    30901 gccccgctca ttgatgtagc ggatcagctt gagcatatgc atcctttctt catcggactg
    30961 ctggtagaaa aaattggtga tgccttcgag gccgggctgg atctccgccc aggcggccat
    31021 tgccaggtag gcctgggacg attccgcttc catgcagacc tgtttgttca gggcgtcctg
    31081 gattgatttg gtgaccatgt tctaaaaatt ttgaggaaag atagggaaat taggagacag
    31141 gatggatctc cctcactcct cactcctcaa ccctcaaccc tcaatcctat tattataact
    31201 ccattcatat attaagccta cttttgcgcc ttccatctta aatcaatcag tccagcaagc
    31261 cataatgcca aaagttacct tcaataccca gcataaatca ttctacagca gccttcaaaa
    31321 aaaagtcgaa acctacttta aggaaaggag gatcaaaaag accggggacc agcgcctttt
    31381 cttcaaggcc ttcctcctga ttttttcagc gggactggtt tatgttttgt tattgacggt
    31441 ccggcttcat cccgtggccg ggcttctttt atgcgggttg atgggtttcc tgctcgcgac
    31501 cataggcttt aatgtcatgc acgatgccaa tcacggaagt ttttcggata aacgatggat
    31561 caacgaggcg ctgtccctga cggcaaacct gatgggggtc aatgcctggt tgtggagaca
    31621 aaaacacaac ctcattcacc atacgtttac caacctggac ggggtggatg aggaccttgc
    31681 caccacgagc ctgctgcgca tgggccctta ccagaaaaaa tacaaggttc accggtacca
    31741 ggctttttat tgtatcccgg tctacggact gacttccctg atgtggactt ttttttcaga
    31801 ttttaaaaag tacttcaccc gtaaaatcca ggttaccgca ttgtcggaaa tgtcgtacgg
    31861 ggagcatatc attttctggt ccagcaaaat cctttatgct ttcttgttta tcgggctgcc
    31921 ggtctatctc ctgggattcc tgccttttct ggccggattt ttagtgatgc atctggtgct
    31981 gggcatcacc ctcgcggtcg ttttccaact ggcccatgtg gtcgaagcca cccattttga
    32041 agacgcccat aaggaggacc tgcatatcgg acaagaatgg gcagtccacc aggtaaggac
    32101 caccgccgat tttgccacag gcaacccggt gatttcctgg ttgacgggag gcctgaactt
    32161 ccaggtcgaa catcatctct tcccccgcat cagtcatatc cactaccccg tgatccagag
    32221 gatggtcaaa gaaacctgtg ccgacttcgg gatccggtac aacggctatc ccaccatgtg
    32281 ggcggccttc cgttcccatc tccggctaat gaagcagttg gggattcagt agaagatggg
    32341 agccgggagt tggaagctgg aagctggaac ctggaagctg gggagggtct actaaacccc
    32401 cggaactgcc aggccaaaac gccctaactt ctaacttcta acttctagtc tcgagcttcg
    32461 agcttcgagc ttggaaactg gaagctaggg gagggtctac taaacctcac ggaactgcca
    32521 ggccaaaacg ccctatcttc tagcttcgag cttctatctt ggaagctggg agctgggagc
    32581 tggaagctag gggagggtct actaaacctc acggaactgc caagctacaa tgccctggct
    32641 tctaacttct aacttcgagc ttctacttgg aagctgggag ctgggagcta ggggagggtc
    32701 tactaaacct cccggaactg ccaggccaaa acgccctatc ttctagcttc gagcttcgag
    32761 cttcgagcat ggaagctgga agctagggga ggggcttcta atcctccgag actgccaggc
    32821 caaaacgccc taacttctaa cttctagctt ctagcttcta gcttctaact tctaacttgg
    32881 aagctgatcc tcacccctca atcctcaatc ctcaatcctc aatcctcaat cctcaatcct
    32941 cctctatctg cctcttgaaa aactgccagg cttcccagtg cacatcgatg tcaccggggt
    33001 aatcgtggcg gccgccaata actttgagaa ggaccacctc cggtttgccc ttttctttaa
    33061 acgtatatct ttcgatggtc ttttcgtcct ccggatcggc gtccggcagg gtggtcttta
    33121 gggggtcccc ccggtatccg gccagtccgg cccaatatcg gaaggaagcc tctgtcgatc
    33181 tgaccattcc catcctgaaa ttcgcagact gcatcattcc acccgcgtaa ggattggtag
    33241 gatcgtgggt gccattgaca atcattacgg ggatggcttt accggcttcc aggcaatcca
    33301 tattaccggc atccggcagg ttggcaatga tggccgtgat cgcccggaaa tggccgggca
    33361 gggtcatcgc cagtttataa cacatatggc cgcctcccga agtccctaca gcaaagacct
    33421 tccggtcgtg gatgccatac ctgactttaa aataggcgat catggcgctg aaaaatcctt
    33481 cttcatcaat attttcctta ttggccaggg agctggcctc tttcctgcat tcgttccagt
    33541 aatttttata tccatccggg tataccagga cggcgttttc cgttttcatt tcttcgataa
    33601 agttcagggt gcgttccatc agggcgcggc cattgccccc ggaaccatgc aggacaaata
    33661 ccaggcttgc accggaaaca ttggccgaag gttgaataaa atgaaacgac cggaaatggg
    33721 gccctatccg gatggaatcg gagaggacct ggccaaagga agaaggcccg atggccagca
    33781 accagaataa aggaaagatt attttcattt tactatggtg tgaaaggcaa acaagaccgg
    33841 tttacagggt ttattttcct ccgctttcgg cggcttcttt tttggcatcg gcgatcattt
    33901 tttcattggc ataaatcagg aattcgaccc tcctgttttg ggcccgccca tcctccgtgt
    33961 cgttggaata tttaggggcg ctttctccaa agcctttcag agcgagacgg gcaggcaaaa
    34021 ccccatgctc ggataaatgc gctgaaacgg aagaggcgcg ccggatggac agggactggt
    34081 tgtatttggc gcctccttta gagtccgtat gcccctggat ctcgatgttc gtatccgggt
    34141 attttttaag gatccccacg agctggttca tcgttttaag ggattcgggg cttagatcgg
    34201 aacggtcaaa accaaacagg attttattgt tgaattccac tacaatgcct tctcccaccc
    34261 ggaccacttt ggcatccggg atctcttctt tgatttcttc cgcctgcttg tccatcttgc
    34321 gcccgatgat ggcgccggcc acgcccccga cggcagcgcc aataatggca cccagcacgg
    34381 tattgccggc aagttttccg actactgcgc cggccgcggc gccaccggcc gtgccgatca
    34441 cggctccttt ttgcgattta ctcatggatt tgcacccgga caccaataaa ggaagaatca
    34501 ggatccagat caggaagtat gggcggttca taatcttttg atttttaatt tcaaaattga
    34561 tttactctcc ggaccaatgt tggtctactt atatcgagga agtcattccc ttctcaatct
    34621 acggcattcc ggcttatttt cgaaaccgga atcatcatct cctgatccgg tcgggctgga
    34681 tgcctgagcc taaggccgtg acccggtctg cccaaacggt taaaccgtcc gtttaccccg
    34741 gatcatcctc cacaagacgg taatcagtgc caggaccagc aggatatgaa tgaggctgcc
    34801 ggcgcggaac ccgatgaacc cgatcagcca gcccatgacg aggattgccg cgaggaggaa
    34861 atgcaggttt cccatggaat tgtgatttta attttacaaa acaaagatcc acctgacttt
    34921 gtgattggat ttatacaata ttggaaaaag attacaggat ttcaatcttc aggtcgtccc
    34981 tgtacttaac cgcctggata ccgggaaatt aaaaggaccg gtatgacccc attcccctga
    35041 aaacggtcac gtttcaataa atattgcctc cggatctgac ccgaacgggg gcggcttgat
    35101 gaaataatgg gcaagcttca tattaggacc ggccatgttg tcgttattgg atagagctgg
    35161 ctgtgaaaaa gtacaggggt tggtccaata aatgcatgaa gcatgaacct gtatctgaaa
    35221 tttgaagtca atagcctctt tcaaaaagtc ctggaaaacg agttgaaggg cctgccggtt
    35281 tcatataccg tgccgggacc gggtgagttg accttccatg aacttgttcc ggatgaggtc
    35341 ctggaggcta tacgagcccg tttggaccaa tacgggattg aaatactgga aaaccccaag
    35401 gcggtgttga ttcagaaaat caaggatgcc attattgaac tggtgtaccg cgaagattca
    35461 ctggccagat gtaaagtgtc cgtgtatttg tctgagcggc ttcggtttgg atatgggtat
    35521 ttgtccaaac tgttttctga atccaccttc acatccattg aaaattatct tattcttcaa
    35581 aaaacggaaa gggcgaaaca tctcattgcc tcccaggccc tgaccttcac ggaaattgcc
    35641 tggaaactca actacagcag cgtggcccat ttcagcaccc aatttaaaaa taccaccggg
    35701 ctgaccccga ccgcttttca aaggattata aataaaaaaa gggcggtcgc cgcccctggg
    35761 cagaacggcg tacagttgtc ggaacatccg gttgataatg ccttttaatc ccctcttatg
    35821 caaaacctac cgatttccgt attgcttgcg gatgatgacg aagacgaccg gatcctgttt
    35881 caggaagccc tgaatgaaat taaatttaaa actgaagtcc ggaccgtggg cgacggcagg
    35941 tctttgatgg aatacctgaa ccggccggaa gtgaagcttc cccaggttat tttcctcgat
    36001 ctcaacatgc cgttgaaatc gggtatggaa tgcctgcatg aactaaaaag ctccgcccga
    36061 ttcggcaaca ttgcggttgc catttattca acctccgctt cggaggagga tatcgaggaa
    36121 acctttgtaa gaggcgcaaa cgtctatatc cggaaaccgg atgacttcaa tgagttgaaa
    36181 aggatcctgg aaaaagtgat caccatcaac tggcaatacc atacctcagg attaaaccgc
    36241 gaaaattttt tacttagttt ttaacggtat tagggtgttt caggacctcc gctcggtgtt
    36301 tacctcctcc ttttttctta acatcatttt tgtttccaca ctctttctgc tcatatttat
    36361 ttccggcatt tcttaccggt ataccacggc cctgaccgat tcctcccggt tggtggttca
    36421 atcccgcaaa acggcgaccg aactgggtca tctatcctcc ttgctggaag aggcacagag
    36481 cagacatgcc gcctttatcc tcaccgggga cacctcgctg atccggccgt acctgcatgc
    36541 cggggttaaa acagggcctg tgcttcagtc gctgaaattg ctggcgggag acaattcgca
    36601 acagatccaa cacctcgata ccctgtcccg gttgatcggg cattgccttg acctgatggc
    36661 cagaacattg gagtggaata ccgggctggc tgctgaccag gcccggttta aagaaagcct
    36721 tatgctgggc gtgaaggaaa tggaacggat ccgggtaaaa atcaaggata tgcttgattt
    36781 tgactccatg cacctgaaag ttcaccagga tatttacgga cgtgaaattt ccctgtcgcc
    36841 cttgtatatc ctgtttgtaa tgctcttctc catgctggtt tttatcttcg cttatttccg
    36901 gatcaataaa gacctcaagc tggaaaaggc gaacaatgaa aaactattgc tggccaatga
    36961 atctatgaat catgccgaag aaatcggtga attcagcagc tggcagtggc acttgcaaac
    37021 caatgctttt gatttttcgg acaatcaata ccgcctgctc gggtaccggc cttttgaatt
    37081 tgaacccaac ctggataaat tcctggaatt tgtccatccg gaagacaggc ataaactcaa
    37141 ggcggcaaga agggagatgt tcagcgggcc gcaaaatccc ttgtccgttg acttcaggat
    37201 ttaccggaaa gacgggtcgc tccgttattt caggtccatc gccaaattat ttaccgacgg
    37261 ggaccgcagg ccggtggtga tcggaataca ctgggatatc acgcaccagc atttaaaccg
    37321 cctggccctg gaagaacgga acctggaact ggagcaaagc aataaagaac tggccacctt
    37381 caatcatatt gccagccacg atttgcaaga gcccctgcgc atcattcaaa cctatgtctc
    37441 ccgcatcatc gactcagagc aagcccggat ttcggaaacc agccagggtt atttcagccg
    37501 gatccttgcc gcattgtcca ggatgcgggc cctgatcaat gacctcctgc tttattccag
    37561 gaccaccaaa acagagaaag tatttcaact ttccgatctg aaccagattt ttgagaatgc
    37621 ccggcaggag ttggcccagg tcctggaaga taaacaggct gagcttagct cagacccatt
    37681 gccggaagcg aaggtcatac cctttcaaat ccagcaaatg tttgtcaacc tgataggcaa
    37741 ttcgttaaaa ttctcgaggt tgggagtaaa gccaaggatc catctgtcct gttctgaggt
    37801 cccttcggat ccttaccggg agcaccatac ggggccggtg aaaaaatttt acaaactgac
    37861 cctgacggac aatggaatgg gatttgagca acagtatgcc cggaaaattt tcgatttatt
    37921 tcaccgcctc cacgggagag acgaatttac gggatcgggc atcggccttt cgatctgtaa
    37981 aaaaatagcg gaaaaccatg gaggggaaat caccgcagag ggccgcccgg gcgagggagc
    38041 tgccttcacc atcctgctgc ctcactgaaa tagatttaaa attttgataa tataagttta
    38101 acaggcatct gttgaattgt gtaatgtttt tattgaatcc tgtaaaagcc cggagctttc
    38161 ttgaaaatat atttgttggg atcattcatt tttaaattct aaaagcttca tcatgaaaaa
    38221 tccatctcat cagcaggccc gtgcaaacca gcctccctcg gttttacccc tggccattcc
    38281 tgctcattcg accattgccg aaggcacaag tgcccatggc acaaggaccc aaggtcccaa
    38341 agcccatggc ctttataaac tgtaccggag ccagcttttt gacatgtact gggctgaaaa
    38401 ggccttgcta aaacaatttc ccaaaatgat ccgtcacgcg catgcccata aactggcgga
    38461 gaccctctat attctcctgg acagcacgag gaaacacatc aaccgcctgg aagctttgtt
    38521 ttcttccagc aacctcaggc ctgttgaaaa caggtgtaaa gccatggacg ggctgatcaa
    38581 agaaacaacg gaagtcattc aatcgacgga agtgggttac gtcggggacg cggggatcct
    38641 gtcctccggc caaaaaatgg aacattatgg catggccgga taccaggtcc tccaggccct
    38701 tgccgaaacc ctgggcgaag ccagggctgc agccatgctg aaatatactt tagaagaaaa
    38761 gaagcaggcc gctgaagtgt tggcgggcct ggccatggat tacctggtgg aaaacgaatt
    38821 tacaggaacc ggggatgccg gggatcgtta cgacttgcat ccctgaaatt tttcgtaaag
    38881 gacatggtca ggaaaaactc aaacatggaa aaccaggaga tcatcaaagc catcagaaca
    38941 ctgatccagg taaatgatga ccggatccag ggttatgaat cggctatgaa ggaatcgaag
    39001 gaggcggatt taaaatccaa ttttttcagg tttattcaaa gcagccgcag gagcaaaacc
    39061 cagttggtcc gtgtacttat acaatccggg gcttctcctg tccgggaatc caggcagaat
    39121 gggctatttt atggcatgct gatgaatttg aaagcggcgg taagggggag ggaccgcttg
    39181 tccattttcc gctgttggga aaaagtggaa gacctggtcg cggaaatgta tgaacggatc
    39241 cttcaaaacg aaggtttcga ttggcccccg gattatgccg gaatcatcgg atcccagagg
    39301 tcagtcctcg aagcagaccg gaataaagtc aaggttttcc gggaaatcct gacagaagaa
    39361 aattaaatca tgtggtctga caggaccgat gggaacaccc gacctcattt cattttttaa
    39421 tccttaaaac tctttcttat gaactcaatg aaatccgttt tgatcgcact cgccggggtt
    39481 gccctcggtg cagtggcagg tacactcctg gcccctgccc ggggcttcgt gacccagcga
    39541 aaacttgcta aaaaaggcgg ggagtatgcc gaagacctgg agtcgaaatt caacgaaatg
    39601 atcgaccagt tcacacaaaa attccggacc ctgaaaaatg atctggtccg gaaatcggaa
    39661 aatgggaaat ccgaacccga agacagcctg gttcagagcg gtagcctttc gggaaggaac
    39721 aagttccagt gatgcggttt ctgagggttg aactgaaggg atagaccagg ttatgcgttc
    39781 tccataaaca gagagatagt aggttatgta tttctaatcc tgccggggta tccgttccta
    39841 aagcgtatac tccggctttt tattaattat ttgtcaatga attaataacg taagccggtg
    39901 gacccggccg ggaaaaatag gatccgggca aggatcgtta cctaaaacaa agcatttaca
    39961 ccagcgccta tgaacatttc tctgaaatgg ccattttatg cgaagatttc ccttttgcta
    40021 attggaatct atgtcttcat ctctattttg tccgccatcc aggatatcgt actgcccgtg
    40081 atctatggtt tgttgctggc tgttttaatc ggtccgctcg taaataaact ggaagccagg
    40141 ggcatgcctc gctccctagc tattttcagc atactgattc tatttgtggc gggatttata
    40201 ttccttttgg tcctgctttc atcacaggcc agcctgttga gtgaggcctt tccggtgttg
    40261 agcgcaaaat ttgatgagct ggtacagcag ggagtggcct ggggttctga tttttttaat
    40321 atccgcgcgt cgaaaatcaa ccagtgggtg aaatccatcc gcggggatat ttatgaaagc
    40381 agcagcgcgg ttgtcggggc cacgctgact accgcgggag gggtcctggc cacgcttttt
    40441 ctgacgccgg tctatgtttt tatgctgctc tattataaaa gccatcttat tgaattcatt
    40501 taccggctca tgggaaggca tcatgaccaa aagatccggg aaatcctctc cgagaccaaa
    40561 acggttatcc agggttatct gcacggatta ttggctgaat tctggatcat tgccgtactg
    40621 aattctgcgg ggctgctgat cctggggatg gactatgcca tcctgctggg aatcggcggc
    40681 gccttattaa atgtaatccc ttacctgggg gggctgatcg cggtggtgat ttatatagtg
    40741 gtggccctgg tcctcaaaac cccggtgtat gcgctctatg tgtttatcct ctatgtagtg
    40801 gtccagctga ttgacaacaa ttatatcgtt ccgaaaattg ttggatccaa agtgaagtta
    40861 aacgccctgg tatgcctcct ctccgtgctt gcaggctcag cgctctgggg tatacccggc
    40921 atgtttttat ccatacccct gatcgccatt attaaactgg tgcttgaccg gatcccggga
    40981 ttaagcccct ggggcttctt actgggtgaa acccgggcgg gaggcggcat caggaaaagg
    41041 actgcggcca ggccaaccgg accggtaccg gaagggagct tggaggtcag gctttaaagg
    41101 gtttgtattc ctgtcccgcg gaggctgctt ttccccggct taatctctgt gtttaatctg
    41161 gtaaaaaacg ccgagttgga atccccggtt ataggatttg aaattttctg atttattgat
    41221 gttggtgagg ccgaattggt aacgggcatc gatgccaaat ccggtgggag gatggatata
    41281 acccaaacca accagcaagc cggcatccag ggattccaaa ttttctttta tatcggtact
    41341 gagattattg actttggatt ttgccccgac taataatcca agctgggggc cggcctcgaa
    41401 ccggaaacca ttgtcaaaca tatactggaa aagcacgggt aaattcaggt aatccagttc
    41461 agtctttaat cccacattct ggccggagct ttttgctccc tgcactgagt aaaggagttc
    41521 cggctgtagt gctattgatc tgtccaggtg gatatggccc agaataccca ggtgaaaacc
    41581 ggccagtggg tcggagtttg tattggtttc gttgtggatg gtaaaaacgt taaggcctgc
    41641 cttgatgcca aagtttacat gctgggcaga taggtttccc gccgtcaaca gcgtggtcac
    41701 gatgagaaat gcattgattt tcatagaggt ttggatttta attttaacaa aattccggcc
    41761 tccggctggg caatccgtta tagaattaaa tcaaaaactt acatttttat gaaataaggg
    41821 tggcgaaatt tcctcattcc cgggtaataa gggcaaaggg catgttagaa ttaaagcaga
    41881 cggcatgccc ggattttcct ctttaagtga tgtttagata tgccaaattt tataaatttg
    41941 gcatatctcg gttccgaata tttctgtctt tggatttctt tgccggaatt aactttagaa
    42001 gatgaactgg atatctgtgt tttttggact gattgctttg gttttgatca tttatttagt
    42061 catccggaat caaaaggaca gaaaagaatt tgaaaaaata ttgaatacgg actataaaaa
    42121 gaccaaagat gaggaagatg aaatggatga tgaatagata tgccaaattt ttaaaatttg
    42181 gcatatctgc aactactctt tctcctcctt atccagcctt tccacggtga tcgtgcaatc
    42241 cccgatcagg gcggccatag cgccaatggc ggccaatacc ggcgcaaaga gggtgcccac
    42301 gacccccagg gtcaggggga aactgatgat ttcctccccg tctttcttat ggatgcttat
    42361 tttccggata tttccttcct ggatcagttc tttcactttt tttaataggt tttcaccatg
    42421 aacttcgaag gattctttag tatgcttatc cattttaata aaatttattg ggtttaaaat
    42481 taccttgctt tccaaacctg gacccctgat tccggtcagg cggcctggtg atgccgatca
    42541 aaggaacgaa tatgcggtat attcttcata acctgtccgg tttgcctatt gaaaaaccag
    42601 gaccgagaaa ggcaggcaga ccctataact cctcaatccc atataaaaat cccttcattc
    42661 cttctgcacc ggtaattctt tcaccaccgg gtttgtgtct tctgttttga catatttaaa
    42721 aaagaatatt ttcaggatat cggccgtcaa cgtataggcc agcatgatga tcagcagggc
    42781 gatgacttgc tgtacatgag ccagagaaag gccgagggca ggggcaaaag gggagaccgg
    42841 caaccaaatg gtcaggataa aagccaggct gccaaaggcc agaagcaggt ttccgggctt
    42901 gcttcggata aacgattttt tggttcgtat aacaaaaatg atgataagtt cggtaagtac
    42961 cgattccaaa aaccatccgg tctgaaatgc cggtccggtg agtttaaaat aataatagag
    43021 ggtataaaaa gtaatgaaat caaagacgga actgtggatt ccaaagacga ccataaattt
    43081 ccggatcagt ttcatgtccc atttccgcgg tgcaacgagt tcggccttat ccacttcatc
    43141 ggatgcgatg gtcaggaagg gcagatctga aatcgaattg gtcagcagga tttgtttggg
    43201 aagcatgggg aggaagggta aaagaagcga tgccgtggct acactcagca tgttgccgaa
    43261 ggtagcgcca gtggtaataa aaatatattt catcgaattg gcaaaagatg tacgcccttc
    43321 aacgattcca tcggaaagga ccgaaaggtc tttttcgagg agtacaaaat cagccgcttc
    43381 cttggccacg tccactgcat tgttcgtgga aatgccagtg tccgcagcat gtatggccgc
    43441 cacatcattg atcccgtctc cgaggtaggc tacggctaaa tgggatttct gaagggattt
    43501 aacgataagt tccttctggt aaggttcgat ttcagcaaat acgaaggtcg atctggcttt
    43561 taccgtcagg gcttctgggg atagggtatg catttgatct ccggtgatga tggcattatc
    43621 cggaaggcca agtttattag cgatataagc tgctgcatgt cggttatcac cggtgatgat
    43681 cttgatgcct acatttaact ttttaagttt ttgaatggac tccaggctgc cggctttcaa
    43741 tggatcctcc aggattataa aacctgaaaa aatcatctcg ttctcgtcct cgcggcgcat
    43801 tttttctcta tgcagcgttt taatgcaaat tcccaatacc ctgaacccct tcagggaata
    43861 ttccttaaat aatgcattga tgttttgccg gcttacttcg tccaggtctt tgatctcagc
    43921 tccgctttgg acgaagcggc aaacttcgag aatgctgctt accgcgcctt tggtgataat
    43981 cagattgcca ttggggtgtt tcacacaaac gctgagacgt ttacgtataa agtcataagg
    44041 gacttcattg gattttgaat atggtttaaa atcaagagcc aggctccgaa gtgcttcatc
    44101 cacggggttc tgataacctg cctggagacc ggcattcaaa cctgcaaaca gccggaccct
    44161 gggatcaggt ttcccttcgt gatcaactat atcatggacc tgtactgaac cttctgtaac
    44221 ggtccccgtc ttgtccatac acaggatctg gacttctcca aaattgaata tcgaagaaag
    44281 tttttttaca atcacctttt ttttcatcat ccgtttggcc cccacggaca ttgcaaaagt
    44341 caggatggcc ggtaacaatt cgggggccat gccaacagca agtgccaggg caaaaaggat
    44401 ggaatcaaaa acgggtttgt caaaaattaa attggtggtg aggatgacca gggaaagcag
    44461 gacggttatg cgcaaaatga aatatccgaa attccggatt cctttttcga aggccgtttc
    44521 ggtaattctg ctcaggctct gggacaactg gcccaataag gtgttttcac cagtatggat
    44581 gaccagggca cgggccgtac cgctgatcac attggttcct ttccagaggc aatttgtttt
    44641 ttcgtgtaaa ggtttatttt catcaagctg tcccacctct ttttccgcag ggaaagattc
    44701 gccggtcagg gtcgcttcgt tgacatgtaa ttctgaattt tctatgatgc ggcaatctgc
    44761 cgggatcacg tcgcctgcat ttaaaatcag gacgtctcca ggtaccactt cctgcgtggg
    44821 aacctggacc acttttcctt cgcgcagaag atcgtgcttc atttcgatca ggcttcgtaa
    44881 tttttccagt gctcttccag cactgagctc ctggaaaaaa cccagtaacc cggaagccag
    44941 caggataaat aaaatgagaa tggtgtcgga tgcctcgccg atccaagcag aaaggatgac
    45001 ggccaggaga aggagtaaaa ccaggggatt aaaaaattga cggaacaaaa gacggatttc
    45061 ccttctcgcc ctggattcgg tttttaagcg ctgttgttga agccggattc tcaccccggc
    45121 ctggactaag gacaggccat tccttgaagt atccaatatc cggtacaatt cttcaagggg
    45181 aatttgggag aatggtttgt tcttgtccat ttatacaagt agctccagaa ccatctaaag
    45241 tacccaattt cagtaagggg ggggtatcaa aagattcaat ggggaaagtt gaaagccatc
    45301 ccgcatcagg aggtcttcgg gagcgggggg gagcatcatt gcatgagata aacttaggaa
    45361 agaatgggga gtcccgttcc gggccaagcc aaaaaggctc cgcaatgaca ggtcattatt
    45421 taatcgtcag aaggatgacc agtcccaaga gtataagagg agtatgaacc atgagcggcg
    45481 gctcaaaaac cggaaaccgc aagaaataca atgccgcccc ggcaaaacca aaccaaatgc
    45541 acgtactggt aacccagggt ggcaataggt ccaggtcttt tgccgcaagg cccatggccc
    45601 caatggccag gtaggccaat accatataga tggcaaatag aagatttgtc caatccatcc
    45661 aggttttgaa tgagggttcc aggatggtgc ttgtactcaa cttatctgct gcccagacgg
    45721 taaccgtaag ccggaaagcg atattcataa tccagaataa actgccaaaa aggaagcctg
    45781 tgaatgcaag ctgggaatac acaccggccc ctgcccgggt catggcatga gtgagcagct
    45841 ggacgccaaa gagggtaagt aggaccccaa cggtaaatgc accatgtata aaataccagt
    45901 tgggtttgtg tccatgtatg atcgagagat attcacgaac atccttcgtc atccaatgtt
    45961 tgtaaggagg agaaaaagcg ccaatccaga agatcaagcc cccggccagc aacaaaatac
    46021 cggcccaacg catttcagga atattgagag tcattctgaa tcatttaaag tcaggatatt
    46081 gaatttagta cgtttaaaat gcttgtacaa caaacgatat gcacaaattt tgattaactt
    46141 gtttattggc cacaatccgg gccatgcggg cgatatccct ggcctcaggt cagggcacgg
    46201 tacaaagtga tcacaaggat aaaaaccacc aacgaagtgt agatccgctt ggtattcctg
    46261 gccagccagt tgggcaggta aatatcgaaa ttgtccatgc cggagtccga atacttcctg
    46321 gcgaccaggg tcaggggaca ataaaattta aacaggacca gggtcaggcc ttccagaaac
    46381 accaggccaa agccgatcca gagccatcgg tcgagtttat ccgtaagggc tgcgtaaagc
    46441 atataaaaaa taaccacatt gaaaaaaagc cagatcgcgg tgtggatgat tttgaccagg
    46501 ataagtttgg accgggtgtt catggtgaac cagttttatt agcagatgcc cttcaggcga
    46561 tttacaaata acttaaccac catttttcct tatgcacgga tttataatca ttgtaccatt
    46621 tgcataaagg atacagaaac aagacgatcc ccagccagac caggtagacc cagggcaggc
    46681 taaggccttc cccgggaacc aggaaataaa aaggaaaatt ccggatctgg tcatcggcct
    46741 cagccaggct atgtccccgg gcaaggaaaa ggacggcgct caccaggtgg gccaggaaaa
    46801 aatgggcgat ataatagaac agggccaccc ggccaaagac ctggacggtc ctggtgaagc
    46861 catttctcag gctttcaagc caggcaagca tcaacaggga gggtcctatg gtcatacata
    46921 taaaaagcag cgagggaggg tatttatggg tgttgaggaa ggacagcagg gtatagaata
    46981 aattcttttg cggactccag gggaaaggat tgccataaat gttcagatag cggaggacga
    47041 tgaacaaaaa aatggcagcc aacccaaacc gggtcagcat tttttttctt ttttccgccg
    47101 gataatccgg ggaataccag gagcccataa cgtatcccag catcatcagc ccggtccagg
    47161 ccacaaaagg gtagatgatc accagggtat ggttgcccca aagcgggtag agggcgaagt
    47221 tgccggtatg cagcaggtcc cacgcgaaac cggggtcaaa cccctctgcc cgctccagtc
    47281 tgtccagaag gttatgtccg aaaacaatga ccaggcccag ggccaggatg agtttgaaag
    47341 gcaggtgtat gaacaggccg aggaggacca tactgatgcc gatggtccag atcacctgaa
    47401 ggaaaaaata attgtagttt ggattaaagg aaatgaccag ggacataatg aagaggtcta
    47461 cggcgatcag ccacagccct cttttgataa taaaaacgct gagttctttt ttggtcttgc
    47521 ggaaactttg caggtaaatg gatacgccgg agaggaatac aaagaccggc gcgcagaaat
    47581 gggtgatcca tcgtgtaaaa tacaagaccg gggtggtggt ggccaggttg agaggatcgt
    47641 ccgtaaacgc accgatgtgg atgaaatcgc ggacatggtc cagggccatg atgatcataa
    47701 cgatgccgcg gaggacgtcc acggattcaa tccgccggat gacgggtatg gaaggggaag
    47761 gcatattggg gttttgtccg gactccccac cttaatccgg tgagctccgg agctatgaat
    47821 ttagtaaata cccatcagaa atgccaatgt ccggaaatgg tgtaaaacga ccgagggaac
    47881 ccataccgcc tgcaccggat tccagcggtt ggggtcagtc gtccaaatct tcttcctttt
    47941 ccttaaaatc tgagaggggg tcggaaaaat cgaaccgggt ctggtgcagt tctttggtca
    48001 ggaggttaaa cgccgagaga ccatgcagga ccagttcctt atagaccggc ttgtcgctgt
    48061 cggggatggg cagggtcttc acgagccttt caagccctga tacctggttc agggatgctt
    48121 cgtattcctc ctgggaggcg tccaccgcga gtttcagggt atgctcgccg gcaaaccagg
    48181 ccttgatcac gccgaaagga tccctttcat tttttttctg tttttccgga tgcggaaaat
    48241 ttttgaggaa ttccgatttg atggccttgt tgagcagttc aaaggccact ttataggggc
    48301 cgatctgttc cccttcatag accagttcca ctttgcccgt gatcgcaggc atcaccccga
    48361 caaaatcgga taacctggcc gtaccgcttt tttcattgtt gatcagcatc cggcgttcga
    48421 ccgcgctgac caggttctca taggcgctga tgccgaggcg ggcggaaacc ccgctgttgg
    48481 tatccaccag ttcgctggta cgggcttcaa aggagatttg ttccagcagg actttgagga
    48541 ggggagggat gaagatattt ttttgctccg gttttacccg cgcctcctgg gccgtgatgg
    48601 tcatggccgt gccgatatcg gtgggatagt gggtaaggat ctggctgtcg atgcggtctt
    48661 tgagcggggt gatgatgctt ccccggttgg tgtagtcctc cggattggct gtaaacagga
    48721 atagtacgtc caggggaagc cggactttaa agccgcggat ctggagatcg ccttcctgca
    48781 ggatattaaa aagggatacc tggatcctgg gctggagatc gggcaattca ttgatcacaa
    48841 aaatggaacg gttggcccgg gggatcaggc cataatggat cacccgttca tcggcataag
    48901 ggagtttgag gctggctgct ttgatcgggt ccacgtcccc gataaggtcg gcgatgctca
    48961 cgtccggggt ggccagtttc tccacataac gctggtcgcg gtgtacccat tcgatggtgg
    49021 tctggtcgcc catagtccta acctcctccc ttccgtatgg cgataaggga gcaaacggat
    49081 tatcattgat ctcactacct gcaatgaccg ggatgtattc atccagcagc cgcaccaaaa
    49141 ggcgggccag cctggttttg gcctggccgc gcaaaccaag cagcaggatg tggtggcggg
    49201 acaggatagc cctttgaagg tccgggatca ccgtgtcgtc aaagccgtga atgccctcaa
    49261 aaatattgat cttgttctta agggcgtgca ccaggttgtc gcgcatttca tccttgatgg
    49321 gtttggggat ataaccggat cgtttgagtt ctccgagggt caggatatct ttaagattgc
    49381 tcatcttctt tttttcttgc tttttttata atccatgaaa ataaattcac ccagggtgtc
    49441 gagggtgctg taaaaggctt tgccgttgtt ggcccgggtg aacgcatcga caaaactggc
    49501 gagataggaa tcccgggcga ccataaaggt ggtgatgggg atattgagtt tgcggcaggt
    49561 gagggccagg gccagggtgc ggttgacgat cttgcggtcg agcccgtagg catttttata
    49621 atatttttta cccaccttga tgcaggtagg tttgccgtcc gtgatcatca tgatctgttt
    49681 gttgggattt ttcctgcggc gcaggatggc catagccagt tcgagcccgg ccaccgtatt
    49741 ggtatggtaa gggcccacct gcaggtaggg caggtccttc atggcgatct ctttggcgtc
    49801 gtctccaaag gtgatgatgt ccagggtgtc tttggggtag cgcgtggtga tcagttccgc
    49861 gagggccatc gccacttttt tggccggggt gatgcggtct tccccataga ggatcatgga
    49921 atgggagatg tcaatcatca gaacggtgct ggtctggctt tgaaaatagg tttcatagac
    49981 ttccaggtcg tcttccctca ggaccaggtc ttcgatcccg tgctggatct gtgcgttttt
    50041 gagcgattcg gtgagggcga tctggtcgag gcggtcgccg aattcataag gcctgatttc
    50101 ggaagtggtt tcatccccca ggccggtgcg cgtggaagta tgctggcctt tccggctttt
    50161 tttgagctgg tcgaagatgt catcgagggc ttgccgtcgg agggcgattt ccaatttgct
    50221 gctgggcatg ggattaatga tgccttcccg gttttcgttg atataaccct tgtcgatgag
    50281 gtcgcggata aaatcggcga tcccgtattc ttcggtggtc agcttaaatc gtttgtccag
    50341 ttcggtgagc cagctca
//
LOCUS       DAOQXT010000024        49941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_779_length_49941_cov_6.400633, whole genome shotgun sequence.
ACCESSION   DAOQXT010000024 DAOQXT010000000
VERSION     DAOQXT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 49941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49941
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_779_length_49941_cov_6.400633"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..835
                     /locus_tag="PKM27_06095"
     CDS             122..835
                     /locus_tag="PKM27_06095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR06870.1"
                     /translation="MKQDLFTKHWNIYQEIIARNYMQHREFGEEIRQVLDRRFPDQPL
                     RVLDLGCGDARQMADQLKGRAVESYTGFDLAGPALVLAQKNMQGLAQEVQVREGKMED
                     WITREEGAFHLIYSSYAIHHLSDPEKKALLAEVRKKLAPGGLFILIDTYRTGGESREE
                     YLDTYLKQNLDQWATLSAEQKGLVRDHIVNYDFPAILRDMMEWAGEAGFELEESPVYD
                     RFHRMMVLSEGLTESHPIS"
     gene            complement(876..1661)
                     /locus_tag="PKM27_06100"
     CDS             complement(876..1661)
                     /locus_tag="PKM27_06100"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNR06871.1"
                     /translation="MKKHCPWLLFLLFACSQPVKPGFTDLSWIDLTHAFDSTTLYWPN
                     NLKNFEIHTDAEGTTAQGYYYASYSICTPEHGGTHLDAPIHFAEGKLTSDLIPLSSLT
                     GEAVVVDVSQKALADRDYRILPQDIEDWEKTNGRIPDHTIVLFRTGYGKFYPDREKYF
                     GTAQKGPEAIPELHFPAIHPEAAQWLVDQRKVKAVGLDTPSLDYGQSTDFKTHQILMG
                     ANLPGFENLANLDQLPVKGIYIVALPMKIKGGSGGPLRIIAGW"
     gene            complement(1733..2203)
                     /locus_tag="PKM27_06105"
     CDS             complement(1733..2203)
                     /locus_tag="PKM27_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06872.1"
                     /translation="MIRSALRKFSPFKSFLALFLLNLPSGVHAQDEEIAIFNSKGKAI
                     AFLEVSEEQTLYMWSGKPVAYLYKEGGETHIYGFNGKHLGWYEEGILYTHDGDAVGFI
                     EGAMSLFTEFEPIKGFKRFKPSRSFREFPPFKPFFSKYFSKTPLSLFLLEGAEE"
     gene            complement(2317..3777)
                     /locus_tag="PKM27_06110"
     CDS             complement(2317..3777)
                     /locus_tag="PKM27_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR06873.1"
                     /translation="MCLPGNVVPGRPVFDWNKDITQAYRLILELRLDQAGRQLDQVRN
                     AQPENLAVDFIEDYIDFFSLYISEDKGLLEKRKAGEAARLKSIETHGDPNSPYTRYAL
                     GEIHLHWGLVYLKFGSYLPAFQRIRRAYKLLEENNNLFPEFLPSLKSLGILHAMLGTL
                     PDELKWMVRALGGINGTVQQGREELRKVYEYSRSNNYLFSEETMVLYGLIMAHFDNEP
                     EAAWQMVRQSGIDPQKSPLACFVLASLAIKCGYNEQAVDLLEKQPSGPEYFPLQYLDY
                     MLGLAKLRRLDPDADLPLKKFSREFKGLHYLKECFQKLAWFELIRGNETGYYQYMERV
                     RQVGNNAVEEDKQATREARLKRKPDPLLLQARLLYDGGYLDRAQAMLEKNQALLINQK
                     ELRLETAYRLARIYQAQKKFTAAISHYLNVIQEGREDPSYYACAAALYTGMIYETLHH
                     HFLARHYYEQCLAMNPEDYRSGLHQKAKAGIGRIGQ"
     gene            complement(3818..4564)
                     /locus_tag="PKM27_06115"
     CDS             complement(3818..4564)
                     /locus_tag="PKM27_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNR06874.1"
                     /translation="MIKNDVPVAIQLKKLYELQQIDSQINEFEILKGELPVEVSDLED
                     ELAGLDTRCKKLEESIKNVEKEVAKFQSGIKDSKNMIGKYEKQLDTVKNNREYDALMK
                     EIELQKLEIELFQKKIKDTETQSVSKEEALKATKEKIKQKKKDLEAKKVELDEVIAKT
                     EEDVAKLTKKSDKAKKDIDDRLLMAYERIRKNYRNSLAVVTVRRDSCGGCYNKIPPQT
                     QLEISQHKKVLVCEHCGRILVDESIALVEA"
     gene            complement(4643..5443)
                     /locus_tag="PKM27_06120"
     CDS             complement(4643..5443)
                     /locus_tag="PKM27_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNR06875.1"
                     /translation="MTIQEITKLLEIWAPLRLAESYDNPGLLTGDPATKVTGILICLD
                     STEEVVQEAIDKGCNLIVAHHPIIFSGLKKLNGANYVERAVIRAIRHGIALYAFHTNL
                     DNILNGVNYKIANLLGLHDMYVLQPHPVDDPALIMPNGSGIIGGLIKPLSEKDFLQHV
                     KNTLQLKVIRHSAFTGKPVSRVALCGGSGRFLIPAAHKTKADVYLTSDLKYHDFFDAE
                     GRILLGDIGHFESERHTIDLIHDYLIDKSDGTPLVKTSLITNPVQYFL"
     gene            complement(5440..5928)
                     /gene="rfaE2"
                     /locus_tag="PKM27_06125"
     CDS             complement(5440..5928)
                     /gene="rfaE2"
                     /locus_tag="PKM27_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNR06876.1"
                     /translation="MIHPYLPKILTREGLISKARSWKLEMASVVFTNGCFDILHYGHV
                     EYLYQASLLGDYLIIGLNSQDSVHRLKGPHRPIQDEQTRCAVIAALGFVDAVSVFSED
                     SPEELIRAISPDVLVKGGDWAVSKIAGAEWVIAHGGRVTTIPYLEGYSTTAIEAKIKQ
                     QS"
     gene            complement(5906..6961)
                     /locus_tag="PKM27_06130"
     CDS             complement(5906..6961)
                     /locus_tag="PKM27_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNR06877.1"
                     /translation="MKRSSVSSFLRFLLFLSIGIILFYYVFSSQQEAYREQCMQNPAQ
                     AGKCSLWAKLLEDIKKLDRLLIFLVMLGFVISNIMRARRWVIMLRTMGIRAGLWETFW
                     AVHLGYFANLALPRMGELARAGTLARRLHAPLEKILGTIISDRLLDLIVLGLFTGLGF
                     FLQFRILYEFLSRKASISLWHLMVLGIAGLLVMAIFIFLFRTDRFYHPVIEKIKSKIH
                     GLREGVVSVVKTGNPLEVILLSAGIWIMYYSMTLLGLKAFGPTSGITALQGMIVFIIG
                     SLGMIIPTPGGIGPYHFLTMSALGIYGISSIEGFSYANLSFIVVQVVTISLFGIIGMA
                     VLSRTKKTKALDSPLPT"
     gene            complement(6965..7315)
                     /locus_tag="PKM27_06135"
     CDS             complement(6965..7315)
                     /locus_tag="PKM27_06135"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNR06878.1"
                     /translation="MLLQVFKSKIHRARVTEANLNYVGSITLDEELMEAARIYPGEKL
                     QVVNVNNGERLDTYAIKGRRGSGDVCLNGAAARKAEVGDIVIIISYALMTPEEAQSFT
                     PVTVYPDETNRLNP"
     gene            7488..8816
                     /locus_tag="PKM27_06140"
     CDS             7488..8816
                     /locus_tag="PKM27_06140"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HNR06879.1"
                     /translation="MKKLLILFIFFVAVGNTLSAQLKTIASGLNGVIGIRVDDWGNKW
                     VAESGSGAGDGALVVITKTNKKHTVIKDLPSTVNPVNMDVVGPWRAIPMSYGRMAVLI
                     GGCEPLLGPHFGSLMIFNLSGYTPGTDEPLTMDDAEKSIDIGTFSFNASDNMDSSPFS
                     AVQDEDGHWYVTDAGANAIIRVSRNGQNMSVFAKFPEFANPTPVGPPMVDPVPTGIVA
                     LPKYGGFLVTTLTGFPFVNGQAGIYFVDRQGNVSPYLQGLTLLTDIAMDKHENIYVNS
                     FGNFSLETFNFAPASANVIRVRKKSIIDTVATGYGPGPSLSLNYNRRKLFVTSFTGEV
                     LETEIPAESSQALGLESRSIENKMDFSLKTFPNPSTQYVNLSWELQNPDSKLTIQLTD
                     AAGRLWYERKNINPGDRFHRIDLNQIAYGTYMVKLSSGSGSQIQRIIVNH"
     gene            complement(8875..9714)
                     /gene="panC"
                     /locus_tag="PKM27_06145"
     CDS             complement(8875..9714)
                     /gene="panC"
                     /locus_tag="PKM27_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNR06880.1"
                     /translation="MLIFTTVREIRLWLDRHAGGRVVGFVPTMGALHEGHLSLIEASK
                     RECGLTVCSIFVNPAQFNDPRDLALYPRTYEADLALLADSSTDLLFFPSVEEIYPHDF
                     VSVEADLGGLDQVMEGLHRPGHFDGVVRVVHRLLDIIRPHKLFMGQKDFQQFTIIGRM
                     IEQLKMPVRLVVCPIAREPGGLAMSSRNRRLSKPVRDQANLIFQILQYGKSKRPVLSP
                     GQTMDACLEKLRQAGFDPEYFEIVDPETLQKIKQWPESGRLCVACTAVWLEGVRLIDN
                     EFY"
     gene            complement(9876..10310)
                     /locus_tag="PKM27_06150"
     CDS             complement(9876..10310)
                     /locus_tag="PKM27_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06881.1"
                     /translation="MAAKKQSPKPILVPPARPLFQYAVKFICMSHIPGTSQQNKAFLP
                     GHYQTIVNIHNPLTKPVRFRSKLAWPIEISRFLPGELKPDGVATISCENVHEYGLHLI
                     HGFEGFLVIESATSLDVTAVYMAGDKTVSSLDVEQVRERKLR"
     gene            10519..10851
                     /locus_tag="PKM27_06155"
     CDS             10519..10851
                     /locus_tag="PKM27_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06882.1"
                     /translation="MPLTVFDIETYGIRLGFSGSISNTQITLNGADGEGTAYSVVIKF
                     SSNHPLPEDAYNASARMYLSFVPFQHLPHYVDLLRNEAPLKASFNKEHPAGCMIYTGH
                     ETPGEGEE"
     gene            complement(10857..10927)
                     /locus_tag="PKM27_06160"
     tRNA            complement(10857..10927)
                     /locus_tag="PKM27_06160"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10893..10895),aa:Cys,seq:gca)
     gene            11053..12195
                     /gene="galK"
                     /locus_tag="PKM27_06165"
     CDS             11053..12195
                     /gene="galK"
                     /locus_tag="PKM27_06165"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNR06883.1"
                     /translation="MESGIVKDSYFQLFGSSPDWTVRAPGRINLIGEHIDYYGGYVMP
                     AAIDRCTELQIGPGTASKAMLYSRKYGELVSFDKDPVPSSHWSAYPDMALQILRESGY
                     ETAPFRLWIGSDLPAGAGLASSAALLCGFITALNRFNGWSLPASKIAYLAQAAEHRLG
                     TPCGLMDQYACLFGKKDTFLLMDCGDLTIKYIPVDLRQYELILVNTLVHHQLNDGGFA
                     RRRKEGEGALESLKSYFSKTGTYRDFNLDELDEIPVLTDTEARRARHAISEHRRVLRF
                     AERVGEGDWEGAGQCMYETHASLRDDYEVSCAEADFIVEVSRATGAAGARIMGGGFGG
                     CVLCLVDKEKKTFILNQVVEGYVEKFRIAPEVIEVKLGELEISGSE"
     gene            12366..12773
                     /gene="arfB"
                     /locus_tag="PKM27_06170"
     CDS             12366..12773
                     /gene="arfB"
                     /locus_tag="PKM27_06170"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNR06884.1"
                     /translation="MKINLTQLLSACRYETSRSSGPGGQHVNKTETKVTLYFNIGDSP
                     ALSEEQKAMLHAKYPNRINESGELYIHASASRSQSANKEAAILKLYTLVTEALLPRKK
                     RVRTRPTSASKAQRLKVKKIKSEKKEGRRRKFD"
     gene            13130..14791
                     /locus_tag="PKM27_06175"
     CDS             13130..14791
                     /locus_tag="PKM27_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNR06885.1"
                     /translation="MTLTAYDYLIIIAYFLFVVGVGFIIKKKIKSSNDFLRQSTRIPL
                     WITSLAFISANLGAQEVLGMVASGAKYGIMTVHFYWVGAIFAMVFLGVYMMPFYYGSK
                     ARSVPEYLSLRFDEKTRGLNAISFAAMTIFSSGISLYALAKLLEIILHWDFDVSIWIA
                     AGIVMIYTFLGGLTSAVYNEVLQFFLIVLGLSPLVIVALQDVGGWAVIKESLSPELTH
                     AWKYMGSADDNPMGLHFISLIFGLGFVMSFGYWCTDFLVVQRAMIAKNMTAARQTPIV
                     AAIPKMLMPIIVILPGVLALTLMTPALQAKGYSIPLDDQGGLDYTMTLPSMIAHYYPS
                     GLLGVGITALMASFMSGMAGNVTAFNSVFTYDIYQSHIAKNRSDSHYLLVGKLTTVVG
                     ILASMMTAYIAKSFNNINDFLQLVFSFVNAPLFATFLLGMFWKRTTGHGAFWGLVGGT
                     LAAMLTHGLSVAEGKGGWIAEWTVIKSGMGQAFIIASIAFIANFFITILVSLLTKPKP
                     DEALRGLVWSLTPKVAAAEGPWYSKPATLGLILIALTILLNLIFF"
     gene            14794..14994
                     /locus_tag="PKM27_06180"
     CDS             14794..14994
                     /locus_tag="PKM27_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06886.1"
                     /translation="MQPLLKRFDELNFVIGLFFTVLALILLLGYSINAEMKQPITVYT
                     GLAFLLFGGVMMWLRGKSNPSE"
     gene            complement(15009..16181)
                     /locus_tag="PKM27_06185"
     CDS             complement(15009..16181)
                     /locus_tag="PKM27_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR06887.1"
                     /translation="MKLFIKILACHPAQPCRLSLLLGLIAAGLLHPSCKGKAPEAETA
                     GEEPEMEATITTEQFAHSAMKWGTLEEKTFYGYVHASGKVETPPSARASISPMFGGYV
                     QNFNLLPGNRVSKGQVLFYIENPLFIDLQREYLELKEQLTFLKADFENQAALSAENIS
                     SKKEALRAESAYKTSLARFESAGKKLQLMGIPPEKLTISNLVTRMSITAPIPGHITEI
                     MITNGKYINPEDVALQILNSADNHVDLYVLESDIIKIKPGQKIIFTLGQGTDQEYEGT
                     VALVGKEVSEKDRTVEVHAHAGRGVPESAMIPGMFVKAKLLTGSHVSPAVPESAVIRI
                     GQSHIVLAKKEEIQGVIHFEQIEIVPGMSLDGWVEIRHGDLKNKEILVSGSNLIPL"
     gene            complement(16190..17386)
                     /locus_tag="PKM27_06190"
     CDS             complement(16190..17386)
                     /locus_tag="PKM27_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR06888.1"
                     /translation="MKRICILFFLLACTASGKLRSQPAWTLDQAIRTGRQNNLLLAIK
                     SQEINRSRALVNSYQDLDKTSFYFLRDQNNITETGYILNTVGASQHFKLPSYYRMHKK
                     YLESEVAVTESLKDLASYELEKNISLVYHELIYLETRKKKVEQIDSLFELYRLAAAKR
                     FDRGESTLLEKLLADNKKSEYELALEQLQLEIRQAYQQLNYLIRDSSDKTIVVPDSLD
                     LLTAIPPAAGGQPGQEVLERIHLARQSELDWEKQKWKPEMTAEYFMGSNIFNENKFYP
                     GIQIGLALPLWKKAGRAMIDAKTAGHEIARMEADANRIRLQTQYRELLLQAGKHRQAI
                     DYYNRQGLALASQIQRSADLSFRQGILDFFQYVFAVEEVSRVLLNELAARKSYNETII
                     LINYLN"
     gene            complement(17383..20502)
                     /locus_tag="PKM27_06195"
     CDS             complement(17383..20502)
                     /locus_tag="PKM27_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNR06889.1"
                     /translation="MLDKIIRFSINNKFIVLLFTLAILASGIYSLQHIPIGAVPDITN
                     NQVQVITTSRNLSTQDVERFLTIPVELEMTNLPGVQEIRSISKFGLSVVTIVFDEDLG
                     TYIPRQLIAEKLKAAEEKIPQGFGTPFMGPITTGLGEIYQYTLEVAPEYRHRYSTMDL
                     RTLQDWTVKRQLAGIPGVVEINTWGGYLKQYEVSVRPDRLLSLNISIPQVFEALEKNH
                     GIAGGGYIEKNGEAFFIRGQGLAASIHELENISIEVRNGTPIYIKDIGTVKSGYANRF
                     GAITANGNGETVMGQIMMLKNANSNEVIRDIKERIEAVQKTLPAGISINPIVERSELI
                     SRTTSTILENLILGCLIVILVVLILLGNFRSGLVITSVIPLSLMFAISMMYLLGIDAN
                     LMSLGAIDFGIIIDGAVIIVEYISFQINARFSPGMVTSRSESRILIKELSTKGASTMM
                     HSAIFGQIIIVIVFIPILSLTGIEGKMFKPMAMTFCLALVGAMVLCFTYVPALSSVLL
                     RPENPKAFNIGRTIHQFLDRLYHPAIRLAMRLKALTLALSVVLFAVAIRTLNGLGGEF
                     IPTLDEGDFVIQPVLKTGTSLTQTISIITEIEKILQSFPEVEKTASRIGAAEIPTDPM
                     SLEEADVIIKLKPIKTWTSAKTKDELADKFKEKLSVIPGVEFEFTQPIEMRFNELITG
                     VKEDLAIKLYGEDLDELNRLGNEIKNAISGVPGAADIIMDKVSGLPQTLIRYDRDQIA
                     KYGITIAQLNDIISMGFAGMRTGTFFEGEKIFDLVVKLDENYRREIGDIHLLPIPIPG
                     GGQLPLQSFATITVETGPAKISRNHTRRNLGLGINVRNRDLESVVTDIQSIVDEKIKL
                     PPGYTITYGGQFENLRSAKSRLIIAIPIALALILVLLFFAFDSLRDALIIFSAIPLAA
                     IGGIFLLYFRDMPFSISAGVGFIALFGIAVLNGIVLIDHYRELNVKNFANTTELIIQG
                     ARQRLRPVMLTAISAALGFLPMAVSSSAGAEVQRPIATVVIGGLVTSTLLTLVLLPIL
                     YSFFKKQNA"
     gene            complement(20586..20951)
                     /locus_tag="PKM27_06200"
     CDS             complement(20586..20951)
                     /locus_tag="PKM27_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06890.1"
                     /translation="MIYVSGLILCLHTIIPHSHDRVFTAVFEQVADHEFDFFGILKGL
                     FNTDLGEDHLEFFAKAGSQPQVQAVALEGLCALPFIQALTLLPQSSRIHKARFSDPDP
                     PIDTGIKSGLSRRGPPSAI"
     gene            complement(21088..23028)
                     /gene="ftsH"
                     /locus_tag="PKM27_06205"
     CDS             complement(21088..23028)
                     /gene="ftsH"
                     /locus_tag="PKM27_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNR06891.1"
                     /translation="MEEKNNKKNLKKFNFNIYWIYGLIVIFILIMQMMLSQHKQSEID
                     FGQFQQMVRAGDVKKVEVINKEKAYIYLKNESLAKYPNAPQTSPNSEYPHFEFTVGSV
                     ESLENQVYELNKELPESNRIYITHTKRTDWIGPILGWVIPFFLIIAIWMFIMRRVGGG
                     GGGGPAGQIFNIGKSKATLFEKDSTPTVTFADVAGLNEAKEEVMEVVDFLKNPKKYTA
                     LGGKIPKGVLLVGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLF
                     RQAREKAPCIIFIDEIDAIGRARGKNTWQGGNDERESTLNQLLVEMDGFPTDKAVILM
                     AATNRPDVLDNALLRPGRFDRQIGIDPPDLKGREAIFNVHLKNIKLRYDVDPVILAEM
                     TPGFAGADIANVCNEAALIAARRNKTAVDMDDFNYALDRVIGGLEKKNKIISPEEREI
                     IAFHEAGHAVCGWLLEHASPLVKVTIVPRGIGTLGYAQYLPKEEYITRTEALLDRMCM
                     TFGGRAAESIVFNKVSTGAQSDLDQATKMAYSMVTIFGMNDKVGNVSFYGMSQDNFQK
                     PYSDETARLIDDEVRKLVESQFLRAKQLLRDHRDKLDLIAQTLLEKEVLVKSDVERLI
                     GPRPFPEKPSHPVQVFGDNGAV"
     gene            complement(23107..23490)
                     /gene="rsfS"
                     /locus_tag="PKM27_06210"
     CDS             complement(23107..23490)
                     /gene="rsfS"
                     /locus_tag="PKM27_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNR06892.1"
                     /translation="MAKEKKPAVKKAPKDPLNELIIATILDKKGDHINLLDLRGLDGP
                     ADYFIVCEADNITLTKALSDHIYREVKTKLGVTPNHTEGEAQAKWILMDYFTTLVHIF
                     YRDTRKYYEIDQLWSDAKTVEFHQV"
     gene            23581..24318
                     /locus_tag="PKM27_06215"
     CDS             23581..24318
                     /locus_tag="PKM27_06215"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNR06893.1"
                     /translation="MKILLDAIDSTQSFASDLLKDSPVSHGTLVMARFQTQGRGRGAH
                     SWEGECGKNFYGSLVLKHAWPTPPPPFALSQAAALAVRDRIESQTGMQVRLKWPNDVI
                     CAGKKVAGILISNQWKGASWESSILGIGINLNQAGFGEDNPDAASLATLTGKSIPVHE
                     ITNGLMDDLDRYYGFLLRGETSRLATDYYNHLHGSRSAVRMQRTGDKSTFLGQVVRLE
                     NDGKIWIRAGREALKSYDVDQIKFLLS"
     gene            24315..25556
                     /locus_tag="PKM27_06220"
     CDS             24315..25556
                     /locus_tag="PKM27_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNR06894.1"
                     /translation="MKTILLTIGNEVLNGQVVDTNAAWLAQELLAEGLPVVEKWSVAD
                     EIPAIVEALGHCLDKGDLVITTGGLGPTQDDLTCEALAVFLKVGRVFHAPTYEGIEQL
                     FRKLGRLPSASHLDQARLPEGAGILTNPAGTAPGMHFVHMGKHIISMPGVPFEMKAIF
                     NTQVRKLVQQYKTGPKRFSHTFNTVGEGETLLEDLIHDITAGAPSNIRFSFLPDLYRV
                     RIRVDLDSENPEDHAAWLDIIQQVRLKLHRYIAGENETTLELAIGNILREKGWMMGTA
                     ESCTGGMIGHMITRNAGSSEYFKGGILAYSNELKSKFLEVDPLTLEKEGAVSEATVRQ
                     MAAGALDSLDVQVAVAVTGIAGPGGGSAEKPVGTVWIGVADIHGRILSKKIQLRRDRL
                     LNIQAASTMALILLHRFLLGE"
     gene            25575..26915
                     /locus_tag="PKM27_06225"
     CDS             25575..26915
                     /locus_tag="PKM27_06225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNR06895.1"
                     /translation="MEHDLIMPRMGESITEATILRWLLREGDEVDLDQPVLEIATDKV
                     DSEIPSPVKGKISRVLFAENEVVPVGATIALIQVDSELNLPEIPTDEIPMARESGPVE
                     TQVSEPVPYVPPMVSPPEPEPAFRSAPTGSGRFYSPLVRTIAREENISLEELDKLDGS
                     GQEGRVTKQDILNYVDRKKKGLTDTVPPPVVFPQPTPVRPGPVITGEGSGGQSEIIEM
                     DRMRRLIADHMVHSKHTAPHVTSFVEVDLTELVHWRNKVKSEFQKKYNQNLTFTPIFM
                     QAVVEAIRAYPLLNSSVDGYKIIRKQDIHIGMATALPSGNLIVPVIRHADRLNLQGLA
                     LAINDLAARARAGQLKPEEVAGGTFTLTNVGSFGSLMGTPIINQPQVAILATGSIKKK
                     PAVLETVHGDVIAIRHMMFMSMSYDHRVIDGFLGGSFLRKVADLLENFDIAQSV"
     gene            26922..29081
                     /locus_tag="PKM27_06230"
     CDS             26922..29081
                     /locus_tag="PKM27_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR06896.1"
                     /translation="MVNPFRVSSMILFLFLAWESRGQSMEYLRLQAEGLYQSKQYLRA
                     APVFRQLQALAPQDYEVKLKRAVCEYEANNLDTAFALAVSLIPVQKPETQYAGFLAGK
                     IAQSRGDFANSVVYYKQFLGQALPGSKLYLAAIAEIKRCGSALYMNPDLRASLVEPAG
                     DEVNTIWNEVHPVPSVNLDSRIYFSSNREPAGFPLNYSADYESPYDMYSSSLNNGEWS
                     EISALNPVLNTRDHELLSGFNENGRIVYFRRGEKLNRLNLLADTLRAEGMSPATSAID
                     FPHLGAEDELYYFTDSILILSSNRPGGLGGFDLYYSIRSGSQWSSPVNLGIRINSPFD
                     EVSPSLAEDGRTLFFSSNNLNSMGGFDVFTAHFDDHTETWSEPVNFGMPVNSGDDDRY
                     FRMLKNKLGAVFASNRKTGTGGFDIYFAYFRQSPAPQGERSRPLFFAEVPAYKSSQQN
                     PGGSLDPKNPNLPGSGQKAQLKVPVIYYLNDEQLKLPAIQKKMDEVVQLLKQNPAAQV
                     IIEVFSDRSAATISDDLMYSVLRGETVRDYLVQGGVRPSRISVKGYGQFFPVAKEKID
                     GLANPAGKSLNRRIEFTFYHPAPEEAGSIFNQKDLAEVDARMQAPERSRFLELEKGLN
                     YKIMLLSSGNAANFRTDHLDQVFIDKNNLTGKYELMSGVFTTFALANQARKKWMASGY
                     EDALVVPYYSNQRIPPQLLNKWSEKFPDLTQFIYRGE"
     gene            29099..29689
                     /locus_tag="PKM27_06235"
     CDS             29099..29689
                     /locus_tag="PKM27_06235"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNR06897.1"
                     /translation="MKKTKLFLDQSLKNLMTVGTFTRSSSFLCKEIVRQIEFTPGQVI
                     LELGAGDGVITKYLLDKMAPDARLIAFEVNPVFCEELRRLEDHRLTVVDQDAARMHEI
                     LAALNISTVQHVVSAIPFVIFPETVALHIIQAAFDHLEPSGKFVQVHYSLLAKKLYDK
                     IFGSVRIRFVPFNIPPAFLLIMQKASVPAPVQALAQ"
     gene            complement(29640..30515)
                     /locus_tag="PKM27_06240"
     CDS             complement(29640..30515)
                     /locus_tag="PKM27_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06898.1"
                     /translation="MKPARPFPAKLLVSGEFTVIRGGDALAIPFPKFAGIWERGAEPD
                     TSLLPLVQYLQKIDYIDSGRLHDDILEGWQFRSTIPPGYGLGSSGALSAALVYHYGRE
                     ISEDTGILQKQLAGVEDYFHGTSSGFDPLVSYKGQGIAWIGKSLVRVPDPLSSGPLMS
                     FLYVIDSGMARGHIHAISWYLEQLKQPVFLPVVEELNDLNHRLVQAWFEQDLQAAEDL
                     FFQVSGLQWKWFQPLITAGVAGIWQKGLESGKYAVKLCGKGGGGYYLLWSKDPEIGAL
                     IGQEPVPVQERKLSA"
     gene            complement(30544..32592)
                     /locus_tag="PKM27_06245"
     CDS             complement(30544..32592)
                     /locus_tag="PKM27_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNR06899.1"
                     /translation="MDNKKFSPYVPSPTILPEFTWRSVLLGSFFGVVFGAATVYLGLK
                     AGLTISASIPIAVMAISIGRRLFNTSILENNIIQTTGSAGEAIAGGVAFTLPAFLFLS
                     ANEDQSVSGASYFNYMTILSLAMIGGMLGTLLMIPLRRPLIIKEHDRLPYPEGTACAS
                     VLIAGEKGGDFARTAYLGLAVATIYAVLQKIIHFIAETPTFVTQAKNKFLPSATFNAD
                     VTPEYLGVGYIIGPRIAGVLVAGGVLAWLALNPLLATLVPAETIAAQLVKLGYLADIK
                     TPGGPGVWDPENQVFANPEIALYRAFIRQIGAGAVTAGGLMTLIKTIPTIISSFKNSF
                     SSIRENQELNGEGQGRTDRDLNQKVVLFGSLALIVIIALLPGIPGSGILSKLMIGVLV
                     LVFGILFVTVSSRITGLIGTSSNPISGMTIATLMGTALIFIAMGWTGQAYEPMALVVG
                     SIICIAAANAGNTSQDLKTGYLLGATPRYQQLALFIGTLVSSVVIGYTIRYLDQDAEV
                     LRTGVEHAIGSDKYPAPQGTLLATLIKGLLSFNLDWQFVLVGAFMAMVIELCGTNSLS
                     FAVGLYLPLSTTLPIFIGGLVKGLVDWRSKHDTIVIEEDEELSKGKLFATGLVAGGAL
                     AGVIIALLSVKYLDQLEKLSIGARMEAIFGTFGYHFTGALCFAGLAAILFRVARQGKQ
                     "
     gene            complement(32644..34167)
                     /locus_tag="PKM27_06250"
     CDS             complement(32644..34167)
                     /locus_tag="PKM27_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNR06900.1"
                     /translation="MIYGLIVFLVLLAIIVVPQFKNHPKGLAILFGTEMWERFSYYGG
                     RALLILYMTASVTGQNPGLNMSVEEAAALYALYVSGVYMTNLPGGWISDKILGARNSV
                     FWGGIIIAVGNLLIGLTASKTGFYTGLLFNAVGTGLLKPNVSTMVGSLYKPGDSRRDS
                     AFSIYYMGINLGAFFAPLITGYFGQNIKWQYGFVAVAIGMVLGLILFKWGGRFLGEAG
                     LKPTAVPEGVANPTTRLLKKSLLYLTLFIALMAGLHWFGWYRLSIRRLSDIMGSALII
                     IPLVYFFYLFTRGGFDPDEKKRIVAIIIFYLAAALFWGAFEQAGSTLTLFADRNTDNT
                     LFGTPFPSSYWQSVNSMWILILSPLFALLWFTLAKKKREPSTPFKFVLGLFFVGLGFL
                     LLVPAAKISAGGVEKVGIIWLLCTYFLHTVGELFLSPVGLSAMTKLAPERIVGQMMGI
                     WFMGASLGNWIGGRVGGLFETYPVNQIFFYVFVFSAASALILLILTPWTRRLMGEVK"
     gene            34381..35016
                     /locus_tag="PKM27_06255"
     CDS             34381..35016
                     /locus_tag="PKM27_06255"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNR06901.1"
                     /translation="MDIFDFIFHIDQHLEAFIAQYGLLVYGILFLIVFCETGLVITPF
                     LPGDSLLFAAGALAANPAHGLSIWTIIPLLISAALLGDNTNYFIGKYLSNKGVGAKLF
                     GIFTIKQEYIDRTHAFYEKYGQRTIILARFVPIARTFAPFVAGIGKMTYGRFIKYCIA
                     GAILWVVLLTFAGYFFGSLPWVKNNFEIVVLGIIAVSLLPMVIGYFRRNRA"
     gene            complement(35026..35583)
                     /gene="pth"
                     /locus_tag="PKM27_06260"
     CDS             complement(35026..35583)
                     /gene="pth"
                     /locus_tag="PKM27_06260"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNR06902.1"
                     /translation="MKFLIVGLGNPGPDYLYTRHNVGFQQLDFLAGKVKANWASDRFG
                     MRCDLKIKGRSLVLLKPDTYMNLSGKAVLFWMNKLKLVPQQVLVVLDEIHLPLGKLRL
                     RKEGSDGGHNGLKSIEQMLETRAYPRMRIGIGKDFPKGRQSEYVLGAWTKDEQKEMED
                     VFGRGSEGILQFALAGIDKAMSLVN"
     gene            35836..36861
                     /locus_tag="PKM27_06265"
     CDS             35836..36861
                     /locus_tag="PKM27_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNR06903.1"
                     /translation="MFRAKLKDTWRMFRRMTIRKAVNALKVYLSFHLGRWSGTALHWG
                     MPVSFAIEPTTACNLRCPECPSGLRAFTRPTGNLKMDFFRSALDALSPYACNLTFYFQ
                     GEPYINPQFLDMVAYAESRGIYTVTSTNGHFLDDKNVKRTLDSGLSRIILSIDGTTQE
                     TYEQYRKEGDLKTVLQGAERLVKARRESGLDHPYIIFQFLVTSANEHQVSQIHRLAET
                     IGVDEVRLKTAQFYHFENGNDLMPRDERYSRYTRSGDGRYRLKNGLEDQCWRMWHSCV
                     ITWDGKVVPCCFDKDASVRLGDLKLQAFGAIWKGEAYRAFRQKLLRGRKFIDICKNCS
                     EGSRVWA"
     gene            37064..38116
                     /locus_tag="PKM27_06270"
     CDS             37064..38116
                     /locus_tag="PKM27_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR06904.1"
                     /translation="MNAALNNPVFYKSIFQGRLEFGTGKNIEKAFQLYQIRAEQYYRN
                     EVMFKAEQIFNVEFGYIEIPRLVAQANEKTFTNTLNLLEYIAQFAAAGSMGAWLIDQG
                     KVLRHKMIEPQSDKVAVQEYLRGRKLVGTKGKEMEAIHALNLAIEKYEKHSQAYERRG
                     YVNFTLKNYKDALSDFNKSIQFDENNALAYFGRAQVHLYNGDVPKALLDLGLTIQKSL
                     ALQMVHWQARYQKGELHYQLKQNKEAAFELKLFCDRKFDPKDINYLKKRDANYFYGRI
                     LMEQEQFDEAAARFNAALAALDENPKSTKFTKADILLQRGLAKKLAGKNGFMADVKEA
                     ATLGSKEAKKMLQAKA"
     gene            38150..40090
                     /locus_tag="PKM27_06275"
     CDS             38150..40090
                     /locus_tag="PKM27_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR06905.1"
                     /translation="MIRFNSRTVSFVFYFPLLFAGWMCRPASLAAQTQTKAAKLFERA
                     KSDISKNRTSEAILKLEEAIEEDPTYREAYLELFRIWQSLDKPAEAISVLQKAALSVR
                     SGQAPLWYNLAQLELQQGWYAKAHEHFTTYLSLNTPDAATRAIAAWLLSKTGYALKHR
                     KDSLDFRFTPLKGMINTADPEYLPSLDATGKMMVFTRRTGNQEDLWISFLQAEAWSAP
                     SPWAGNSNQNEGAHVISADGKTIVFTACGREGGFGGCDLYSSDFRQGRWTRPRNMGQV
                     ISSRAWESQPSLNANGRILYFVSDRPGGMGGYDIWMSFKGPAGWTPPVNAGPNINTEK
                     DELSPCLHADGLSLYFRSEGWPGFGSQDLFVSRKSREGNWEQPINLGYPLNDHRDQGA
                     MAVSLDGKSAYYTDQRIGSSNQLLASDIIRLELPQPFRSLACTFIRGRISDLSSNAAL
                     PGALVTLAGGEGLFIKDSVVSDPDGEFLLIVPLQHAYQIHAKLDGYNLYSERLVAEEA
                     LAAGYERTHDIKLEQIRPGVEEPLKPVVLENVLFETASFILLPESFNELDGLSELLKQ
                     NPSFSLAIHGHTDNTGDPEFNRTLSGRRAQAVYNYLLTRQIEASRMTWEGFGDRRPIA
                     SNDSEEGRSKNRRVEFILSKGK"
     gene            40138..41544
                     /gene="rlmD"
                     /locus_tag="PKM27_06280"
     CDS             40138..41544
                     /gene="rlmD"
                     /locus_tag="PKM27_06280"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNR06906.1"
                     /translation="MGKSKQMIAEQVRILDMGDQGMGIGKDPEGRVYLVRDAVPGDLV
                     DIEVKKHRNRLGNGRPVRMLESSSWRTHPFCSHFGVCGGCKWQHMSYEGQLSYKFKNV
                     ADALKRIGGLPDPQVRPICGAPEDRYYRNKLEFSFGNSRWLEPDELTREGAPDRNALG
                     FHRPGSFEKIVDITHCHLQPEPSNAIRNAVRTWAKEKGHTFYDVKNHSGFLRNLIIRT
                     TTLGQVMVTLVTGEDLPEALEQINQLLLLQFPQITTLYHIHNPKKNDTIFDLDLHLVH
                     GPGYILESIGQLQCRIGPKSFFQTNSIQAARLYGFVEEFAKLTGHEIIYDLYSGTGTI
                     GLFLAGRCRQVIGVEEVPEAVEDAYLNASLNQIKNVVFHTGDVRKMMMEGLFGREGKP
                     DLIVLDPPRAGLHQDILPLLIDSGAPKIIYVSCNPATQARDLKVLCTAYEHRISQPVD
                     MFPHTAHIENVTLLELKS"
     gene            41550..41900
                     /locus_tag="PKM27_06285"
     CDS             41550..41900
                     /locus_tag="PKM27_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06907.1"
                     /translation="MQVSTEKFLVLQEELKVYRNALNKALDIMLEQGITEYPIFIAHQ
                     ATFHGGIPIIKRSEVKGNWNINVSSLEEFIQKKIISSRKVASFKQSYRPATDYICFFV
                     LSELGAQFIFLPRV"
     gene            41918..42790
                     /gene="rfbA"
                     /locus_tag="PKM27_06290"
     CDS             41918..42790
                     /gene="rfbA"
                     /locus_tag="PKM27_06290"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNR06908.1"
                     /translation="MKGIILAGGLGTRLFPLTLAVSKQLMPVYDKPMIYYPLSILMMA
                     NIRDVLIISTREDLPGFEKLLRDGSDLGCHFSYMVQPEPNGLAQAFVLGESFIGREAS
                     CLILGDNIFYGAGLGNILKSCQQPDGGIIFAYQVSDPERYGVVEFDENYKALSIEEKP
                     EHPRSNFAVPGLYFYDHSVIEVAKNLKPSPRGEYEITDVNKYYLERGKLYVKAMGRGF
                     AWLDTGTHQSLMQAGQFIQVIEERQGLKIGCIEEVAYQMKFIDASQLENLAQRYLKSG
                     YGQYLLGLLHHKFF"
     gene            42802..43953
                     /locus_tag="PKM27_06295"
     CDS             42802..43953
                     /locus_tag="PKM27_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNR06909.1"
                     /translation="MAEELEKGPEDHEDEQTRSVLKSFKSSKIWLPIFIGLAVVVYML
                     SANFDPTALASIKWNPHTTLWLFLAVAFLVIRHLAYALRLYYLAGDTIGFWKCIKLII
                     IWEFSSAVSPTALGGSAVAVFVLSQEKMGTSRTLAAIIYTVVLDSLFFVASVPLLLLV
                     IGANVINPSYVDFSSLDKVAFSLFFFYGLILVYGLFFAYGLFYNPRQFKKILVFFTTW
                     PLLNRFKGAAQKLGDEMMDSSYQIAHRDRRFHLKSFLLTATAWACRFLIVNCLIIALI
                     DPVSYHPWDQLKIYGRQVIMFVFMMFSPTPGAAGFAEVFFGSFIGDYIPKSAALIIAL
                     IWRFMTYYLYLVAGVLVIPAWLTGVIRNRKEARAKVAQEKASRFQNPES"
     gene            43992..44528
                     /locus_tag="PKM27_06300"
     CDS             43992..44528
                     /locus_tag="PKM27_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNR06910.1"
                     /translation="MLNRRLQTFLAIILGAAILMLARYFYFQPGVKVGEECPPFQTRL
                     ADGTAFEMASLKGKYVLIDFWGSWCAPCRKEAPELKKFYESWRQTGFRDGSGLELVSI
                     AIEEDRSNWEKAIREDSATWPVHILELDLFNSSLVKTFQVREIPTKILLDPSHRVVAV
                     NQGFEEMAVLLNSRIQKD"
     gene            44597..45112
                     /locus_tag="PKM27_06305"
     CDS             44597..45112
                     /locus_tag="PKM27_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNR06911.1"
                     /translation="MEQEGKDILEESGVQPEDMADPVENQPDVEQVANEEETTVRDLK
                     DKYLRLLAEFDNYKKRTSKERIEFAKFAAQDLVVSLLPVLDDFDRAVKHGQVSEGTSL
                     IHHKLVQVLKQKGLEEMQSQGQTFDPALHEALTEIEVPEDQKNKVIETLEKGYYLADR
                     IIRFAKVVVGK"
     gene            45119..46267
                     /gene="dnaJ"
                     /locus_tag="PKM27_06310"
     CDS             45119..46267
                     /gene="dnaJ"
                     /locus_tag="PKM27_06310"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNR06912.1"
                     /translation="MAKRDFYEVLDISRDADEITIKKSYRKIAMQFHPDRNPGDKAAE
                     EKFKEAAEAYGILSDPDKKARYDRYGHAGVDPNMGGGFSGGGMNMEDILRNFGNIFED
                     SPFESFFGGGGRAEAGGQRGSSLRISVTLDLHEIATGVSKKISVKKQVTCNTCGGSGA
                     KDKSSKKTCNTCHGSGYVRQVRNTFLGQMQTTTTCPQCHGSGQVITSHCATCKGDGRV
                     MGEDTFDIDIPAGVEDGMQLSVRGKGNVGAKGGPSGDLIIQIKEKAHESLKRDGSNLV
                     YDLYLNFADAALGASIDVPTLDGKASIKVPAGTQAGKVFRLKDKGLPSVQSYQKGDLL
                     VQVNIWTPKQLNEEEKEILEKLKQLPNFQPNPGKAEKGFFEKMKSFFS"
     gene            46264..46749
                     /locus_tag="PKM27_06315"
     CDS             46264..46749
                     /locus_tag="PKM27_06315"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNR06913.1"
                     /translation="MRPALFIGILASLFVSCDPPDYIFEESRSFIGQPWVYPDSAVYR
                     FNIKDTTRYYNLLLELDHGRNYPYENLYVRFETVFPDASVKSDVVSISLADETGAWYS
                     DCRGRTCTFYLTLQEQAIFSNTGEYQIKVKPWMRSDTIEDVYRLAFRVERGDKRPKIK
                     Q"
     gene            47135..48361
                     /locus_tag="PKM27_06320"
     CDS             47135..48361
                     /locus_tag="PKM27_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06914.1"
                     /translation="MFLRANINDPLTRHFLTKNVERQFLIFKIGIVTFFLMLGVDFIN
                     WQKGLLNTNPYVQATTIFRYLALSFFISVYLLRKRYNSYTPNKLRFHVTLNIFLFVLT
                     LFPRSLLIMMDRQVTGSYYIFILILNVVLLLDLRPRILLMGISLILMLLTIHQAANPY
                     EILKISLAIEVSFATVLSTYVGHLLYRAEATNFYAKKDLEEKHLALERSLIQLEKQNE
                     ELNSSRLKVQEQNLLLEESKIKLETYTQALEQNRALLEEQNKALEENKSRLQESLAFS
                     QNQLTGYALSMAQKNDLLLQLKSSLEKPDASHHQALRLVDQGIGEDDIWKRFHHHFEQ
                     LHPEVVRRLENLYPELSASDLRLFALLKLKITTKEIADLLGISPESANTARYRLRKRL
                     GLNEGEDLQQFVDRFE"
     gene            complement(48445..48864)
                     /locus_tag="PKM27_06325"
     CDS             complement(48445..48864)
                     /locus_tag="PKM27_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06915.1"
                     /translation="MEKYHLDHDISILYVQASSFPEGISAAWESLHSRVKDSQGRKNF
                     GISFMDRTGGIIYRAATEEAYPGEADQYGCETFKIKKGDYLSVLIPDFMENIPSIGQV
                     FRKLTAESSIDPEGVAVEDYFNEKDVRCMVRLLDEPA"
     gene            complement(49014..49361)
                     /locus_tag="PKM27_06330"
     CDS             complement(49014..49361)
                     /locus_tag="PKM27_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNR06916.1"
                     /translation="MSEFPPCPKCRSEYTYADGGLLICPECFNEWNPEEAAPAAAENT
                     NPKILDSNGKELLDGDTVMVIKDLPVKGSPRPIKAGTKVKNIRLTDGDHNIDCRIEGF
                     GAMGLKSEFVKKV"
BASE COUNT        12106 a        12661 c        12954 g        12220 t
ORIGIN      
        1 tgaaaaagca ggaggctaaa gttgaaaacg gtcaaccaaa ttcaggcatg gacagtttac
       61 cacccaccaa ccaccaccca ccacccacca accaccaccc accacccacc aaccaccacc
      121 catgaagcaa gacttattca caaaacactg gaacatttac caggaaatta tcgcgcggaa
      181 ttatatgcag caccgggagt tcggagaaga gatccggcaa gtgctcgacc gtcggtttcc
      241 cgaccagccg ctccgggtac tggacctggg ctgcggggat gcgcggcaaa tggccgatca
      301 attgaaaggc agggccgtgg aatcttatac cggttttgac cttgccggcc ccgccctggt
      361 tttggcccaa aagaatatgc aaggcctggc ccaggaggtt caggtcaggg aaggcaaaat
      421 ggaagattgg atcactcgag aagaaggagc atttcatctg atctacagca gctatgccat
      481 ccaccacctg tccgaccctg aaaaaaaagc cctcctggcg gaggtccgaa aaaagcttgc
      541 ccctggtggt ttatttatcc tcatcgatac ctaccgcacg ggaggggaat cgagggaaga
      601 atatttggat acctacctca aacagaatct ggatcagtgg gcgaccctca gcgccgaaca
      661 aaaaggcctg gtcagggacc atattgtgaa ttatgatttt ccggctatac tcagggatat
      721 gatggaatgg gccggtgagg cgggtttcga gcttgaagaa agtccggtgt atgaccgttt
      781 tcaccggatg atggttctaa gtgaagggtt gactgagagt caccctattt cttaagaact
      841 aaaagatatt ccaaattttc aaaatttgga atatctcacc accctgcaat gatcctcaac
      901 ggccccccgc tgcctccttt tattttcatg ggcaaggcca cgatataaat tcctttcacc
      961 ggcaattgat ccaggttggc caggttttca aaaccgggca gattggcgcc catcaggatc
     1021 tggtgggttt taaaatcggt ggactggcca tagtcgaggc tgggcgtatc caagcctacc
     1081 gccttgacct tcctctgatc caccagccat tgggccgctt cgggatggat ggcggggaag
     1141 tgcagttctg gaatcgcttc aggtcccttt tgggcggtcc caaaatactt ttcacggtcc
     1201 ggataaaatt ttccgtaccc ggtccggaaa agcacgatcg tatggtccgg tatccggcca
     1261 tttgtttttt cccaatcttc aatatcctgg ggcagtatcc ggtaatcccg gtcagcaagc
     1321 gccttttgtg aaacgtccac cactacggct tcgcctgtca ggctgctgag gggtattagg
     1381 tcggacgtga gttttccttc cgcaaaatgg atcggtgcat caaggtgggt gcctccgtgt
     1441 tccggagtac agatggaata ggaagcataa taatatccct gggccgtggt tccttcggca
     1501 tcggtatgga tctcaaaatt tttaaggttg ttgggccagt acagggtggt actgtcgaac
     1561 gcatgggtga gatcgatcca gcttaggtcc gtgaatcctg gttttaccgg ttgcgagcag
     1621 gcaaaaagaa ggaacaagag ccagggacaa tgttttttca tggtcaggtg gatagttttt
     1681 taatgaacat ttaaaggtac gaactgaaag ggaggaaggg aggaagggga ctttattctt
     1741 ccgctccttc cagcaagaac agcgataagg gggtcttcga aaaatacttt gaaaaaaaag
     1801 gtttgaaggg ggggaattcc ctgaacgacc tggagggttt gaatcgtttg aaacctttga
     1861 tcggttcaaa ttcggtgaaa aggctcatgg ccccttcgat gaatcctacg gcatcgccat
     1921 cgtgggtgta caggatacct tcttcgtacc agcccaggtg tttgccgttg aagccgtaaa
     1981 tatgcgtttc gccgccctcc ttatacaggt aggcgaccgg ttttccgctc cacatataga
     2041 gggtctgctc ctcgctcacc tcaaggaagg caatggcctt tccctttgaa ttaaaaattg
     2101 cgatctcttc atcctgggca tgaacgccgg atgggagatt cagaagaaat aaggcaagga
     2161 aggatttgaa tggtgaaaat ttacgaagcg cagaccggat cattgcaaac aaagttcggt
     2221 aaatgtcaga atatactgac tcctgacccc tgactattga ctcctgaccc ctgactattg
     2281 actcttgact cttgactctt gactcttgac tattgtttat tgcccaatcc tgccgatacc
     2341 cgcctttgcc ttttgatgca ggccggaccg gtaatcttcc ggattcatgg caaggcattg
     2401 ttcataatag tggcgtgcca gaaaatggtg atgcagggtc tcgtaaatca ttccggtgta
     2461 aagggccgcg gcgcaggcat aataggaggg atcttcccgg ccctcctgga tgacgttcag
     2521 gtaatggctg atcgcagccg taaatttttt ttgcgcctgg taaatccggg caagccggta
     2581 ggcggtttcc agccggagtt ctttttgatt gatcagcagg gcctgatttt tttcaagcat
     2641 cgcctgggcc cggtccaggt aacctccatc atacaggagc cgggcctgga gcaggagcgg
     2701 atccggcttc cttttcaggc gggcttcgcg ggtggcttgt ttgtcttctt ccaccgcatt
     2761 gttacccact tgccggaccc tttccatata ttggtaataa ccggtttcat ttccgcggat
     2821 cagttcaaac caggccagtt tctgaaaaca ttctttgaga taatggaggc ctttgaattc
     2881 acggctgaat ttttttaaag gaaggtcggc atcggggtcc agccggcgca gtttggccag
     2941 gcccagcata taatcgaggt attggagggg gaaatattcc ggtcccgaag gttgtttttc
     3001 caaaaggtct acggcttgtt cattgtaacc gcacttaata gccaggctgg ccagtacaaa
     3061 gcaggcgagg gggctttttt gcggatcaat tccactctgc cggaccattt gccaggctgc
     3121 ttccggttca ttgtcaaaat gtgccatgat caggccataa agcaccatgg tctcctccga
     3181 aaacaagtag ttgttggaac gggaatattc gtatactttt cgaagttctt cccgtccttg
     3241 ttggacggtt ccgttgatgc cgccgagagc ccttaccatc cattttaatt catccggtaa
     3301 agtacccagc atggcatgga ggatacccag gcttttaagg ctagggagga attcaggaaa
     3361 gaggttgtta ttttcttcca gcagtttata ggcccttcgg atccgttgga acgccggcag
     3421 gtaagatcca aatttgagat aaaccagtcc ccagtgcagg tggatttctc ccagggcata
     3481 gcgggtataa ggcgagttgg gatctccatg ggtctcaatt gactttagcc tggctgcctc
     3541 tccggccttc cttttttcaa gcagcccttt gtcctcactg atatagaggc tgaaaaagtc
     3601 aatataatct tcaataaaat caacggccag gttttccggt tgtgcattcc gaacctgatc
     3661 aagctggcgc cctgcctggt caagacggag ttcaaggata agcctgtaag cctgggtaat
     3721 gtctttattc cagtcaaaaa ccggacggcc cggcactaca ttgcccggga gacacaagga
     3781 aaatattaaa aagcttttaa ataatggctg ggagggctca ggcctcaacc agtgcgatgc
     3841 tttcatccac aagtatacgc ccgcaatgtt cacaaaccaa tactttttta tgttggctga
     3901 tctccagttg ggtctggggc gggatcttat tataacagcc tccgcaggaa tcgcggcgca
     3961 cggtgaccac cgccagggaa ttgcggtagt ttttacgtat gcgttcataa gccatcagca
     4021 accggtcgtc aatatccttt ttggctttgt ccgatttttt ggtgagtttg gccacatctt
     4081 cctccgtctt ggcgatgact tcgtccagtt ccactttttt ggcttcgagg tctttctttt
     4141 tctgcttgat tttttctttg gtggctttca aggcttcctc cttggacacg gactgggttt
     4201 cggtgtcttt tatttttttc tgaaagagct cgatttcaag tttctgcaat tcaatctctt
     4261 tcatgagggc gtcgtactcc cggttgtttt tgacggtatc gagttgtttt tcgtatttgc
     4321 cgatcatgtt tttggaatcc ttgatgccgg attggaattt ggccacctcc ttttccacgt
     4381 ttttgatgct ctcttccagt tttttgcagc gggtgtccag cccggccagt tcatcttcca
     4441 ggtcgctgac ttcgaccggc aattcacctt ttaagatctc aaattcattg atctgggaat
     4501 cgatctgttg cagttcatag agttttttta attgaatggc tacgggaaca tcgtttttta
     4561 tcattggata tcagtgtttt agaagtgaat gaacctggtg ctttgaaaaa agcgatgcaa
     4621 aagtacgatt tttttgcaaa aatcacagaa aatactgtac cggattggta atcaggctgg
     4681 ttttgaccag gggagtaccg tctgatttgt ctatcagata atcgtggatc aggtcgatgg
     4741 tgtgccgttc actttcgaaa tgaccaatat cgcccaggag gatacggcct tccgcatcaa
     4801 aaaagtcatg gtatttaagg tctgaagtga ggtagacatc ggctttggtt ttgtgggccg
     4861 ccgggatgag aaaacgtccg gatccgccgc agagggccac ccgggaaacg ggcttgccgg
     4921 tgaaagccga atgccggatc actttcagct gaagcgtatt tttgacgtgc tgtaaaaaat
     4981 ccttttccga aaggggcttg atcaggccgc cgataatccc ggaaccgttg ggcatgatca
     5041 gggcgggatc atcgaccgga tggggctgca agacatacat gtcgtgcagt ccaaggaggt
     5101 tggcgatttt atagtttacc ccattgagga tattgtccag attggtatgg aaggcataaa
     5161 gcgcgatgcc gtgccggatg gcccggatga ccgcccgctc cacatagttt gccccattca
     5221 gtttttttaa gccgctgaaa atgatcgggt gatgggccac gatgaggttg cagcctttat
     5281 ctatggcttc ctgcaccacc tcttcggtac tgtccaggca gatgaggatc ccggtaactt
     5341 tcgtggcggg gtcgccggtc agcaggcccg gattgtcata agactcggca aggcggagcg
     5401 gggcccagat ttccaatagt ttggttattt cctgtatggt catgattgtt gtttgatttt
     5461 tgcctctatc gcagtggtgg aatagccttc aagataagga atcgtcgtca cccggcctcc
     5521 atgtgcaatg acccattcgg cccccgcgat cttcgaaacc gcccagtctc cgcccttgac
     5581 caggacatcg gggctgatgg cccggatcag ttcttcgggg ctgtcttctg aaaaaaccga
     5641 taccgcgtcc acaaaaccca gggcggcaat gactgcgcag cgggtttgtt catcctggat
     5701 ggggcggtgg gggcccttaa gcctgtggac ggaatcctgg ctgttgaggc caatgatcaa
     5761 atagtccccc agaaggctgg cctggtaaag gtattccaca tgtccatagt gcaggatgtc
     5821 gaaacaacca ttggtgaaga cgacgctggc catttccaat ttccaggacc tggcctttga
     5881 aatcaagccc tcccgggtca ggattttagg taggtagggg tgaatcaagg gctttggttt
     5941 tttttgttct cgataaaacg gccatcccga tgattccaaa aagagagatg gtaaccacct
     6001 ggaccacgat aaagctcagg ttggcatagg aaaagccttc aatggaactg atgccgtaaa
     6061 tgcccagggc gctcatggtg agaaaatgat aaggcccgat gcccccgggg gtgggaatga
     6121 tcatgcccag gctgccaatg ataaaaacga tcatgccctg caaagccgtg atgccggagg
     6181 tggggccaaa agctttgagg ccaaggaggg tcatcgaata atacatgatc cagattcctg
     6241 cggaaagaag gatgacttca agggggttcc cggttttaac cacactgacc accccttccc
     6301 gcaggccatg aattttactt ttaatttttt caatgacggg atgataaaat cggtcggtcc
     6361 ggaataaaaa aataaatata gccatgacca ggagaccggc aattcccagc accatcaggt
     6421 gccacaggga gatgcttgct ttccggctga gaaattcata aagaatcctg aattgaagaa
     6481 aaaatccgag gccggtgaac aagccgagca caataaggtc aagcaaccgg tcggagatga
     6541 tggtgcccag tattttttcg agcggggcat ggagccttcg tgcaagggtg ccggcccggg
     6601 ccaactcacc catgcggggc agggccaggt tggcaaaata acccagatgc acggcccaaa
     6661 aggtctccca aagtccggcc cggatgccca tggtccggag catgatcacc cagcggcggg
     6721 ctctcatgat attgctgatc acgaagccaa gcatgaccag gaagatcagc aggcggtcca
     6781 attttttaat gtcttccagc aacttggccc agagcgaaca tttgccggcc tgagcaggat
     6841 tttgcatgca ttgctcccgg taggcttcct gctggctgct gaaaacataa tagaacagaa
     6901 tgatgcctat ggataaaaac aacaggaacc ttaaaaagga ggaaaccgac gatcttttca
     6961 tacttcaggg attcagccgg ttggtttcgt ccggataaac ggtgaccggg gtaaaagact
     7021 gggcttcttc cggggtcatc agggcatatg aaataatgat tacgatatcc ccgacttccg
     7081 ctttacgggc cgccgctcca ttgaggcata cgtctcccga ccccctcctg cccttgatgg
     7141 cataggtatc cagccgctcc ccgttgttga cattgaccac ctgcagtttt tcaccgggat
     7201 agatcctggc agcctccatc aattcttcat caagggtgat gctccccaca taattcaaat
     7261 ttgcttcggt aacccttgca cggtggattt tggacttaaa aacctgaagt aacattgagg
     7321 caaagttaag gattggttcc gttttttggc ttccgcacct gcaggagccg gatggtcgcg
     7381 tgcccaaatt cggggcgcaa gtaatattat gattttttat actatattcg ccttgagaaa
     7441 ccttgctttt ataaccatac tgtttaatat cacctaaaaa ccatatcatg aaaaaacttt
     7501 taatcctgtt tatctttttc gtggcagttg gtaataccct gtccgcccag ttaaaaacca
     7561 ttgcttccgg gctcaatgga gtcatcggca tcagggtcga tgattggggc aacaaatggg
     7621 tcgccgagtc cggaagcggc gccggtgatg gagccctggt ggtcattact aagaccaata
     7681 aaaaacatac ggtgatcaag gaccttcctt ccacagtgaa tccggtcaat atggatgtag
     7741 tgggcccctg gagggcgatc cccatgtctt atggcaggat ggctgtgctc atcggaggat
     7801 gtgaaccgct cctcgggccc catttcggct cgctgatgat ttttaacctc tccggatata
     7861 ccccgggcac ggatgagccg ctcaccatgg acgatgccga aaagagcatt gacatcggta
     7921 ctttctcctt caacgcttcg gacaatatgg attccagtcc gttttccgcc gtacaggatg
     7981 aggacggtca ttggtatgtg acggatgccg gagccaatgc catcatccgg gtgagccgca
     8041 atggccagaa tatgtccgtc tttgccaaat tccctgaatt tgcaaatccc acgccggtcg
     8101 gccccccgat ggtggacccg gtgcctaccg ggatcgtggc cctgcccaaa tatgggggct
     8161 tcctggtcac cacgctcacc ggattcccgt ttgtgaatgg ccaagctggg atctattttg
     8221 ttgaccgtca gggcaatgtg agtccttacc tccagggttt gaccctgttg actgacattg
     8281 ccatggataa acacgaaaat atttatgtga attcttttgg aaatttctcc ctggagacct
     8341 ttaattttgc gcctgcaagc gcaaacgtga tcagggtaag gaagaagtca atcatcgata
     8401 cggtagctac cggttatggt cctggcccca gtctttccct gaattacaac cgaagaaaac
     8461 tgtttgtgac gagttttacc ggcgaagtgc tcgaaaccga aattccagct gaaagcagcc
     8521 aggcgctcgg acttgaatcc aggtccattg aaaacaagat ggatttttca ctgaaaacct
     8581 ttccaaatcc ttccacacaa tatgttaatc tgagttggga actgcaaaat ccggacagca
     8641 agctaaccat ccagttaacc gatgccgccg gcaggttgtg gtacgaaagg aaaaacatta
     8701 atcccggtga ccgttttcac cgcattgatt tgaatcaaat tgcttacggt acatacatgg
     8761 tgaaactgag ctcaggctca ggaagccaaa ttcaaagaat tattgtaaac cattagcagc
     8821 ttcaattacc gtttacatta aggagggcag gataacgatc ttgccctttt ttatttaata
     8881 aaattcattg tcgatcaaac ggacgccttc cagccatacc gccgtacagg cgacgcagag
     8941 cctcccggac tcgggccatt gcttgatttt ttgcaaagtc tccgggtcca cgatttcaaa
     9001 atattcagga tcaaaaccgg cctgccgcag tttttccagg caggcatcca tcgtctgccc
     9061 cggagagaga accggccttt ttgattttcc atactgaagg atctggaaga tcaggttcgc
     9121 ctggtccctt accggcttgc tcagccgcct gttcctcgaa ctcatggcca gcccaccagg
     9181 ctccctggca atcgggcata caacgagccg gaccggcatc ttcagttgtt cgatcatcct
     9241 ccctattatg gtaaattgct gaaaatcctt ttgccccata aacaacttat gcggccttat
     9301 aatgtccagc aaacggtgga ccacccggac cactccatcg aaatggcctg gccggtgcag
     9361 gccttccatc acctggtcca ggccgcccag atccgcttcc accgaaacaa aatcgtgcgg
     9421 atagatttct tccaccgaag gaaaaaaaag caagtccgtg gaggaatccg ccagcagggc
     9481 aaggtccgct tcataggtcc ttggataaag agccagatcc ctgggatcat taaactgggc
     9541 aggattgacg aaaatgctgc atacggtcag gccgcattcc cttttgctgg cttcgatcag
     9601 ggataaatgg ccctcatgga gggcccccat ggtcggaaca aatccaacga cccggccccc
     9661 cgcatgccgg tccagccaaa gcctgatctc acggaccgtg gtaaaaataa gcatggacga
     9721 atgtaacggc tgaatggtca ttctccaact aacaaacttg caaacttgct cacttacaaa
     9781 cttgccaact tgctaactga taaactgaca aactaaaaag ggacctgcat ccgcagcgtc
     9841 cctaaacctt aaacttatat gaatgattaa aaaacttacc ggagttttcg ttccctgacc
     9901 tgttcaacat ccaggctgga aacggttttg tcgccggcca tgtagaccgc cgtgacatcg
     9961 aggctggtgg cgctttcgat cacgaggaat ccttcaaagc cgtggatcag gtgcaagccg
    10021 tattcgtgaa cattttcaca ggagatggtc gcgaccccgt ccggcttgag ttcacctggg
    10081 aggaaacggg aaatttcaat gggccaggcc agtttggacc ggaacctgac cggtttggtc
    10141 aggggattgt ggatgtttac gatcgtctgg taatgaccgg gcaggaaggc tttgttttgc
    10201 tgggaagtgc ccgggatatg gctcatgcaa ataaatttta cggcatattg gaacaagggt
    10261 cgcgcaggag gtacaagaat gggtttggga gattgttttt tagcagccat aatcaggtgt
    10321 tttagatgat ttataatatg atgtaaaaag gaagtaaatt attacccctg aaatgttaaa
    10381 aaatcattca gtggcagaaa tggccctgcc aggggctcca aaaatgcatt ccgctgcggg
    10441 caaagcagaa aaaataatcc ggatttaagg cctccctgta cccggcgatc gttactttca
    10501 cgttttaaaa tctttattat gcctttgacc gtttttgata ttgaaactta tggcatccgg
    10561 ctgggttttt ccggatcgat cagcaatacg cagatcacgc tgaatggggc cgatggggag
    10621 ggcaccgctt attccgtggt tatcaaattt tcttccaatc atccgctgcc tgaagatgct
    10681 tacaatgcga gtgcaagaat gtatctcagc tttgttcctt ttcagcattt accccactat
    10741 gtcgatttgc tgcgtaatga agcgcccctg aaagccagtt ttaataaaga acatccggcc
    10801 ggctgcatga tatataccgg gcatgaaacc cccggcgaag gagaagaata aaaaggaggt
    10861 cggaagcgga atcgaaccgc tgtacaaggt tttgcagacc tctgcctgac cgctcggcca
    10921 cccgacctgt tgaacgaggg gcaaatataa ggagatttat ccattgccat ggtagccctt
    10981 tccccggatg aatggtaaaa tttgggtaaa aaaaagattc tccaagtatc ttggacctta
    11041 atttattcga aggtggaatc cggcattgta aaagactcct atttccagtt gtttggctct
    11101 tccccggact ggaccgtcag ggcccctggc cgcatcaacc ttatcgggga acatatagat
    11161 tattacgggg gctatgtgat gcccgctgcc attgaccgct gcacggaatt gcagatcggt
    11221 cccggtacgg cttcgaaagc catgctgtat tccagaaaat acggggaatt ggtaagcttt
    11281 gataaagatc cggtcccctc cagccattgg tcagcctatc cggatatggc tttgcagatc
    11341 ctgcgcgaat cgggttatga gacggcgcct ttccggctct ggatcggttc cgacttaccg
    11401 gcaggcgccg gcctggcatc ctctgcggcc cttttgtgcg gattcattac ggcattgaac
    11461 cggttcaacg ggtggtccct cccggcttcc aaaatcgcct accttgccca ggcggccgaa
    11521 caccggttgg gcaccccttg tggactgatg gatcagtatg cctgcctctt tgggaaaaaa
    11581 gatactttcc tgctcatgga ttgtggtgac ctgacgatta aatatatccc cgtcgacctc
    11641 aggcagtatg agctcatact ggtcaatacc ctggtgcatc atcagctcaa cgacggggga
    11701 tttgcccgca ggcgcaaaga aggggaaggc gcgctggaat ccctcaaatc ctatttttca
    11761 aaaacaggga cttaccggga ttttaatctg gatgagcttg acgagatccc ggtgctcacc
    11821 gatacggaag cccgcagagc caggcatgcc atttccgaac accggagggt ccttcgcttt
    11881 gcggaaaggg tcggagaggg ggattgggaa ggcgccggtc aatgcatgta cgaaacccat
    11941 gccagcctgc gcgacgatta tgaagtatcc tgcgcggaag cggattttat tgtcgaagtt
    12001 tccagggcga caggggccgc cggggcccgg atcatggggg gaggatttgg gggttgtgta
    12061 ctctgcctgg tggacaaaga gaaaaaaact tttattttga atcaagtcgt agaaggatat
    12121 gttgaaaaat tcaggatcgc gcctgaagta atcgaagtca aattgggcga gttggaaata
    12181 tccggaagcg aataaatccc caaatcccaa atcccaaatc ctacaatcct acaatcctat
    12241 caatcctaca atcctatcaa tcctatcaat cctattaatc ctattaatcc tatcaatcct
    12301 attaatccta ttaatcctat taatcctatt aatcctatca atcctattaa tcctagcatc
    12361 ctaccatgaa gataaacctg acccaattgt tatccgcctg ccgctacgaa acctcccgga
    12421 gcagcggtcc gggtggccag cacgtcaata agaccgagac caaagtcacc ctgtatttta
    12481 atatcggtga ttcgccggcc ctctcggaag agcaaaaagc catgttgcac gccaaatatc
    12541 ccaaccggat caacgagtcg ggagaactct atatccacgc atctgccagc cgttcccagt
    12601 ccgccaacaa agaagcggcc atcctgaagt tgtacaccct ggtcacagaa gccctcctgc
    12661 cccggaaaaa aagggtcagg acccggccaa cctcagccag caaggcccaa cggctgaagg
    12721 tgaaaaaaat aaaatcggag aagaaagagg ggaggaggag gaagtttgac tgagcattag
    12781 aagctggaag ttagaagtta gaagctgggg acgccggctc ctgggattat tgcatattca
    12841 gagattttcg ccagcttcgg ttcgagcttc gagcttccag ctcccagctt cgagcttcga
    12901 gtttcgagtt tcgagcttcc agctccgagc tcccagcttc caagttagaa gttagggcgt
    12961 tttagcctgg cagtctcgga ggtttagaag tccctcccct agcttccagc tccgagcttc
    13021 cagcttcgag cttccagctt cgagccccca gcttcgagcc cccagcttcg agcttcgagc
    13081 ttcgagcttc cagctttttt ctatctttcg aaaaacaagc gaaaccccaa tgaccttaac
    13141 cgcatacgat tacctgatca tcattgccta ctttcttttt gtggtggggg tgggattcat
    13201 catcaagaaa aaaatcaagt cgagcaacga cttcctccgg cagtcgaccc ggatccccct
    13261 ctggatcacc agcctggctt ttatttccgc caatctgggg gctcaggaag tgcttggcat
    13321 ggtcgcttca ggggcgaagt acgggatcat gaccgttcat ttttactggg tgggcgccat
    13381 tttcgccatg gtcttcctcg gggtgtacat gatgcctttt tattatggga gcaaagcgag
    13441 gagcgtaccc gaataccttt cccttcgttt tgatgaaaaa acgcgagggc tgaatgcgat
    13501 ctcttttgca gccatgacta ttttttcttc gggcatttcg ctttatgccc tggccaaact
    13561 gctggaaatc atattgcatt gggattttga cgtttccatt tggatcgcgg ccggcatcgt
    13621 gatgatctat acctttctgg gtggcctgac cagcgcggta tataatgaag tcctgcagtt
    13681 ttttcttatc gtactgggcc tttcccccct cgtgatcgta gccctgcagg atgtgggcgg
    13741 ctgggcggtg atcaaagaat cgctgtcccc ggaactgacc catgcctgga aatacatggg
    13801 ctcggcggac gacaatccca tggggctgca ttttatttcc ctgatcttcg ggctgggttt
    13861 tgtgatgtcc tttggctact ggtgcacgga tttcctggtg gttcagaggg ccatgatcgc
    13921 caaaaacatg acggccgccc gccaaacccc catcgtggcg gccattccta aaatgctgat
    13981 gcccatcatc gtcatcctgc ctggggtcct cgcccttacc ctgatgaccc cggcccttca
    14041 ggccaaaggt tattccattc ctcttgatga ccagggcggc ctcgattata ccatgaccct
    14101 gccttccatg atcgctcact attatcccag tggattgctg ggagtgggca tcaccgccct
    14161 gatggcttct ttcatgagcg gcatggcggg gaacgtgacc gccttcaact cggttttcac
    14221 ctacgacatt taccagtccc atattgccaa aaacagatcg gacagccatt accttctcgt
    14281 cggcaagctg actacggtgg tcggcatcct ggcctccatg atgaccgctt atattgccaa
    14341 gagcttcaac aatatcaatg acttcctgca attggtcttc agcttcgtaa acgccccgct
    14401 gttcgcaact tttttactcg ggatgttctg gaaaaggacc accgggcacg gcgctttctg
    14461 gggactcgtc ggcgggaccc tggcggccat gttgacgcat gggctttctg tggcggaggg
    14521 caaagggggc tggatcgcag aatggacggt gatcaagagc ggcatgggcc aggcttttat
    14581 tatcgcctcc atcgccttta ttgcaaattt cttcataacc atcctcgtca gcctcctgac
    14641 caaacccaaa ccggatgaag cgctccgcgg actggtctgg tccttaactc ccaaagtggc
    14701 tgcagccgaa ggaccctggt acagcaagcc ggccaccctg ggcctgatcc tgatcgcgct
    14761 taccatattg ttaaacctta tctttttctg atcatgcaac ccctcctgaa acgatttgac
    14821 gaactcaatt ttgtgatcgg tctttttttt accgtcctcg ccctgatcct gttgctgggt
    14881 tattccatca atgcggagat gaaacagccc attacggttt ataccggcct ggccttcctt
    14941 ttattcggag gggtcatgat gtggctcagg ggtaaatcca atccctcgga ataaccggat
    15001 cttttacctc agaggggaat aaggttgctt ccactgacca ggatctcttt gttttttaaa
    15061 tcaccatgcc tgatctcgac ccagccatcc agggacatgc cgggaacgat ttcaatttgt
    15121 tcaaaatgga tgacgccctg gatttcctct tttttggcca ggacgatatg gctttggccg
    15181 atgcggatca cggccgattc gggcaccgcc ggactgacat ggcttcccgt cagcaatttc
    15241 gccttcacaa acatgcccgg gatcatggcc gattccggga cgccccggcc ggcatgggca
    15301 tggacttcca cggtacggtc cttttcgctg acttccttcc cgacgagggc gaccgtgcct
    15361 tcatattcct ggtcggttcc ctggccgagg gtgaatatta ttttttggcc gggtttaatt
    15421 ttaatgatgt ccgattccag cacataaaga tccacatggt tatcggcgga attcaggatc
    15481 tgcagggcca cgtcttcggg gttaatgtat tttccgttgg tgatcatgat ctcggtgatg
    15541 tgtccgggta tgggggcggt aatgctcatg cgggtcacca ggtttgaaat ggtcagtttt
    15601 tcaggaggaa tgcccatcag ctgcaatttt ttaccggcac tctcaaaccg ggccagggaa
    15661 gttttatagg ccgactcggc gcgaagggct tctttcttac tgcttatgtt ttcggccgac
    15721 aaggcagcct ggttttcaaa atccgctttt aaaaaggtca attgttcctt taactccaga
    15781 tattcccgct gcaggtcgat aaagagtgga ttttctatat aaaacaaaac ctgccctttg
    15841 ctgacccggt tgccgggaag gaggttgaaa ttttgtacat atcctccaaa catggggctg
    15901 atggaagccc tggccgaagg aggcgtttca accttgccgg aggcatggac atatccgtaa
    15961 aaggtctttt cctccagcgt gccccacttc atggcgctgt gagcgaattg ttcggtggtg
    16021 atggtagcct ccatctccgg ttcttccccg gccgtttcag cttcgggtgc tttgcctttg
    16081 cacgacgggt gaagcaggcc ggcagcgatc aatcccaata acaggcttag gcggcagggt
    16141 tgcgcggggt ggcaggcgag gatctttata aacaatttca tggtcgcgtt taatttaaat
    16201 agttgatgag gataatggtt tcgttgtatg attttcgggc ggcgagttca ttcagcaaga
    16261 cccgggacac ttcctccacc gcaaacacat attggaaaaa atcaaggata ccctgtctga
    16321 acgaaagatc ggccgatcgc tggatctggc tggcgagggc caggccctgc cggttgtaat
    16381 aatcaatggc ctgccggtgc ttgccggcct gtaaaagcag ctcgcggtat tgggtttgta
    16441 gcctgatgcg gttggcgtcc gcttccatcc gggcgatttc atgccctgcc gttttagcgt
    16501 ctatcatggc gcggccggct tttttccaca ggggcagggc gaggccgatt tgaattccgg
    16561 gatagaactt gttttcgttg aatatattgg aacccataaa atactctgcg gtcatttccg
    16621 gtttccattt ttgtttttcc caatccagtt cggattgcct ggccaggtgg atgcgttcca
    16681 gcacctcctg gcccggttga ccgccggctg ccgggggaat ggctgtaagc agatccaggg
    16741 agtccgggac cacgatggtt ttgtcgctgc tgtcccggat gaggtaattc aactgctggt
    16801 aggcctgcct gatttccagt tggagttgct ccagggccag ctcgtattcg ctttttttat
    16861 tgtccgccag cagtttttcc agcaaggtgc tttccccgcg atcaaagcgc ttggcagctg
    16921 ccagtctgta aagttcgaag aggctgtcta tctgctcaac cttttttttc ctggtttcca
    16981 ggtagatcag ttcatgataa accagggaga tgtttttttc caattcgtaa ctggccaggt
    17041 ccttcagact ttccgtaacc gcgacttcgg attccaggta ttttttatgc attctgtaat
    17101 aggaaggcag tttaaaatgc tgggaggcgc ctaccgtatt caggatatat ccggtttcgg
    17161 taatattgtt ttggtccctc agaaaataga aggacgtttt atccaaatcc tgatacgaat
    17221 tcaccagtgc ccttgaacgg ttgatttcct gggatttaat cgccaggaga aggttgtttt
    17281 gccggcccgt ccggatcgcc tggtcgaggg tccaggcagg ctgggaccgg agtttcccgg
    17341 aggctgtaca ggctaaaagg aaaaacagga tgcaaattct tttcatgcgt tttgtttttt
    17401 gaaaaaactg taaagaatgg gtaggagcac cagggtaagc agggtggagg tgaccaatcc
    17461 tccgatgact acggtcgcta tcgggcgttg tacttcggcc ccggcagaag aggataccgc
    17521 catgggcagg aagccgagtg ccgcggatat ggccgtaagc atgactggcc ggagccgctg
    17581 ccgggcgccc tgtataatga gttccgtcgt atttgcgaag ttcttaacat tcaattcacg
    17641 gtagtggtcg atcagcacaa tgccgttgag gaccgcaatg ccaaaaaggg cgataaatcc
    17701 cacccctgcg gaaatactga aaggcatatc cctgaaatac aataaaaaga tgcccccgat
    17761 cgcggccagt gggatcgcac tgaatatgat gagggcgtcg cggagggaat caaaggcgaa
    17821 aaaaagaagg accaggatca gggccagggc aatcggtatg gcgatgatca accggctttt
    17881 ggcggacctc aggttttcaa actggcctcc gtaggtgatg gtatatcccg ggggcaattt
    17941 tatcttttca tcgacgatgg actggatatc ggtcaccacc gattcgagat cccggttccg
    18001 gacattgatg cccagaccga gattcctgcg ggtatggttc ctggatattt tggccgggcc
    18061 ggtttctacg gtgatggtgg caaaggattg cagcgggagt tgacctccgc ccgggatagg
    18121 aatgggaagg aggtgaatgt ccccgatttc ccggcggtaa ttttcatcca gttttaccac
    18181 gagatcgaaa attttttccc cttcaaaaaa ggtaccggtc cgcatgcccg cgaagcccat
    18241 ggagatgata tcgttgagct gtgctatggt gatcccgtat ttggcgatct ggtcacggtc
    18301 gtaacggatc agcgtctggg gcaggccgct gactttatcc atgatgatgt ccgccgcgcc
    18361 cggaaccccg gaaatggcat tcttgatctc attgcccagc cggttcagtt cgtccaggtc
    18421 ctctccatac agtttgatcg caagatcttc cttcacgccg gtgatcaact cgttaaaacg
    18481 catttcgatg ggttgggtaa actcaaattc gaccccgggg atcacggata atttttcctt
    18541 gaatttatcg gcgagttcat cctttgtttt tgcagaagtc cacgttttga tcggtttcag
    18601 tttgatgatc acatcggctt cctcaaggga catcgggtct gtggggatct cagcggcccc
    18661 aatccggctg gccgtttttt ccacttcggg gaaggattga agtatttttt caatttcagt
    18721 aatgatggaa atggtctggg tcagggaggt ccccgttttg aggacaggct ggatcacaaa
    18781 atcgccttca tccagggtag ggatgaactc accgcccagt ccgttcaagg tcctgatggc
    18841 taccgcaaac aatacgacag acagggccag ggtcagtgct tttaatctca ttgcaagccg
    18901 gatcgccggg tgatagaggc ggtccagaaa ctggtggatc gttcttccga tattaaaggc
    18961 ttttggattt tcgggcctga gcaaaacgga ggacagggcc ggcacatagg taaaacaaag
    19021 gaccatggct ccgaccaggg ccaggcagaa tgtcatggcc atgggtttga acatcttgcc
    19081 ttcgatcccg gtcagggaga ggatgggtat aaaaacgatc acgatgatga tctggccgaa
    19141 gatggccgaa tgcatcatgg tggacgcgcc tttggtggaa agttccttaa tcaggatcct
    19201 ggactcggac cggctggtga ccatacccgg actgaaccgg gcattgatct gaaaggaaat
    19261 atattccaca atgatcaccg ccccatcgat gatgatccca aaatcgatgg cccccaggct
    19321 catcaggttg gcatcgatgc ccagcaaata catcatggag atggcaaaca tcaggctcag
    19381 cgggatcacg gaagtgatga ccaggcccga gcggaaatta cccaggagaa tgagcaccac
    19441 caggatgacg atcaggcaac ccaggatcag gttttccagg atggtggagg tggtccggga
    19501 aatcagttca ctcctttcca caatgggatt gatgctgatg ccggcaggca gggttttctg
    19561 aacggcttca atcctttcct tgatatcccg gatgacttcg tttgaattgg cgtttttcag
    19621 catcatgatc tggcccatga ccgtttcccc gtttccattc gccgtgatgg cgccaaaacg
    19681 gttggcgtat ccggatttga cggtgccgat gtctttgata tagatggggg tgccgttccg
    19741 gacttcaatg ctgatgttct cgagttcgtg gatgcttgca gcaagcccct gcccccggat
    19801 aaaaaaagct tccccgttct tttcgatata tcctcccccg gcgatgccgt ggtttttttc
    19861 cagggcttca aaaacctggg gaatggaaat gttaaggctg agcagccggt cgggacggac
    19921 ggagacctcg tactgtttga gataacctcc ccaggtattt atttccacca ctccggggat
    19981 gcccgcgagc tgcctcttta cggtccagtc ctgcagggtg cggaggtcca tggtcgaata
    20041 ccggtggcgg tactcagggg caacctcaag ggtatactgg tagatttctc ccaggccggt
    20101 ggtaatcggc cccataaaag gggtgccgaa tccctgcggt attttttctt cggcggcctt
    20161 gagtttctcg gcgatcagtt gccggggaat ataagtgccc agatcttcgt caaaaacgat
    20221 ggtcaccacc gacaagccaa acttggatat ggaacggatc tcctgcacac cgggcaggtt
    20281 ggtcatttcc agttctaccg ggatggtcag gaaccgttca acatcctggg tcgaaaggtt
    20341 cctggaagtg gtgatcacct ggacttgatt gttggtaatg tcgggtaccg ccccgatggg
    20401 aatatgctgc agggaataaa tcccggaagc caggatagcc agggtgaata gcagtacaat
    20461 gaatttattg ttgatgctaa accggatgat tttatcaagc atgatgcagg aaaaaatgaa
    20521 aaaagcggta taaaataaaa tgaacggtca ccggcttaaa aatccggtga agggtatgcg
    20581 gaaaactaaa tggccgaagg agggccccgc ctggagaggc cggattttat cccggtatca
    20641 atgggtggat ccgggtcgct gaaccgggcc ttgtggatac gggatgactg agggagaagg
    20701 gttaaagcct gaataaaggg aagggcacac aggccttcca atgcaacggc ctgaacttga
    20761 ggttggcttc cggctttggc aaaaaattcc aggtgatctt ctcccaggtc cgtgttgaaa
    20821 aggcctttga ggatgccgaa aaagtcaaat tcatgatcgg ccacctgttc gaacaccgcg
    20881 gtgaaaaccc ggtcgtgact atgaggaatt attgtatgaa ggcagaggat aagccccgat
    20941 acataaatca atataagtct gattgctttg atattacaaa gataatccgg aatccggagt
    21001 ccacatcacc ttgagccttc aatcttctcc ctcaaccctc aaccctcaac cctcaaacct
    21061 caaacctctc tcctcaaacc tcaatcctca aaccgcccca ttgtccccaa acacctgcac
    21121 cggatggctg ggcttttcag ggaaaggtct tgggccgatc agtctttcca catcagactt
    21181 gaccaggact tctttttcaa gaagggtctg ggcgatgagg tcgagtttgt cgcggtggtc
    21241 acggagcaat tgtttggcgc ggagaaactg ggattccacc agtttgcgca cctcatcatc
    21301 gatcagcctg gcggtttcat ccgagtaggg cttctggaaa ttgtcctggc tcatgccata
    21361 aaaagaaaca tttccgactt tgtcattcat gccgaaaatc gtgaccatgc tgtaagccat
    21421 cttggtcgcc tggtccaggt cgctctgcgc accggtggaa accttattga atacgatgga
    21481 ttcggctgcc cttccgccaa aggtcatgca catccggtca agtagggctt ccgtacgcgt
    21541 aatgtattct tccttgggca ggtattgggc ataaccgagg gtgccgatcc cccggggtac
    21601 gatggtcacc ttcaccagcg gggaggcatg ttccagcaac catccgcaaa ccgcatgacc
    21661 ggcttcatgg aaggcgatga tctcccgctc ttcaggtgaa atgattttat ttttcttttc
    21721 gagaccgccg atcacccggt ccagcgcgta attaaaatca tccatgtcca ccgcggtttt
    21781 gtttcggcgg gcggcgatga gggcggcttc attgcacacg tttgcaatgt ccgcgcctgc
    21841 aaaaccgggg gtcatttcgg cgaggatgac cggatccacg tcgtaccgga gtttgatgtt
    21901 tttaaggtgg acattgaata tggcttctct tcctttgagg tccggcgggt caataccgat
    21961 ctgccggtca aaacgccccg gcctgagcag ggcattgtcg agtacgtcgg gacggttggt
    22021 agctgccatg aggatcacgg ctttatcggt ggggaagcca tccatttcca ccaggagttg
    22081 gttcagggtg ctttcccgtt catcattgcc tccctgccag gtatttttgc ccctggccct
    22141 tccaatggcg tcgatctcat caatgaatat gatgcagggc gctttctcgc gagcctgtct
    22201 gaagaggtcc ctcactcttg aggccccgac tcccacaaac atctctacaa aatcggagcc
    22261 cgatatggaa aagaagggta cgccggcttc tccggccacg gccttggcga gcagggtttt
    22321 acccgttccc ggagggccta cgagcaatac gcctttgggt atttttccac ccagggcggt
    22381 atattttttc ggattcttga ggaagtccac cacctccatg acttcctcct ttgcttcgtt
    22441 gaggccggcg acatcggcga aggttacggt tggagtggaa tctttttcaa aaagggtggc
    22501 tttggacttt ccgatattaa agatctggcc tgccggtcct cctccaccgc ccccgccgac
    22561 tcggcgcatg ataaacatcc agatggcgat gatgaggaaa aaggggatga cccaacccag
    22621 gatcggtccg atccagtcgg tccttttggt atgggttata tagatccggt tggattccgg
    22681 caattccttg ttgagttcgt acacctggtt ttccagggat tcgacagaac ctacggtaaa
    22741 ttcgaaatgc gggtattcgg aattgggtga ggtctgcgga gcattgggat atttcgcaag
    22801 gctctcattt ttgaggtaga tataggcttt ctccttattg atgacttcca cctttttgac
    22861 atccccggcc ctcaccattt gctgaaactg cccaaaatca atttcggatt gtttgtgctg
    22921 gctcagcatc atctgcatga tgaggataaa aatgacgatc agtccatata tccaatagat
    22981 attgaaatta aatttcttca ggttcttctt attgttcttc tcttccatgc tgctttttca
    23041 aatttaacag ttatttcata aacattttta accgggtatt cgattcgggt gaaaggttaa
    23101 agtattctaa acctggtgaa attcgacggt cttggcgtca ctccacaact ggtcaatttc
    23161 atagtatttc cgcgtgtccc ggtagaaaat atggaccagg gtggtaaaat aatccataag
    23221 gatccacttg gcctgggctt cgccttcggt atggtttggg gtgacgccca gtttggtctt
    23281 gacctcccgg tagatatggt cagagagcgc tttggtcagg gtgatattgt ccgcttcgca
    23341 aactataaaa taatccgccg gtccgtccag gccccgcagg tccagcaggt tgatatgatc
    23401 gcctttttta tccaggatcg ttgcaatgat gagttcgttg agggggtctt tgggtgcctt
    23461 tttgacggca ggtttttttt ctttggccaa gcgctgattt tattttcttt ggaaaatgta
    23521 ggttatttgt tttacaaaag tatggaaatt agggcatttc ggggccaggg aataggtaaa
    23581 atgaaaatcc tcctggacgc cattgattcg acccaatcct tcgcctcgga tctcctgaaa
    23641 gactccccgg tttcccacgg caccctggtc atggcccggt ttcagaccca gggacgcgga
    23701 agaggcgcgc attcctggga aggggagtgc ggaaaaaact tttatggcag tcttgtttta
    23761 aagcatgcat ggcccacccc tcctcctccc tttgcactca gccaggcagc ggccctcgcg
    23821 gtccgggacc ggatcgaaag ccagaccggt atgcaagtcc gcctgaaatg gcccaatgat
    23881 gtgatctgtg cgggaaaaaa agtggccggc atcctgatct ccaatcaatg gaaaggggcc
    23941 tcctgggaat ccagtatcct tggcatcggc atcaatttga accaggccgg ttttggcgag
    24001 gataacccgg atgcggcctc cctggccacc cttacgggaa aatccattcc ggtccatgaa
    24061 atcaccaatg gcctgatgga tgacctggat cgttattatg gtttcctgtt gcgcggagag
    24121 acgagccggc tcgcaaccga ttattataat cacctgcatg gaagccggtc ggccgtccgg
    24181 atgcaacgaa ccggcgataa aagcactttt cttgggcagg tcgtcagact cgaaaacgat
    24241 ggcaaaatat ggataagggc cgggcgggag gccttgaaat cctacgatgt ggatcaaatc
    24301 aaatttctcc tttcatgaaa accattctat tgacgatagg caatgaagtg ctcaacggac
    24361 aggtggtcga taccaacgcg gcctggcttg ctcaggaatt attggcggaa ggcttgcccg
    24421 tcgtggaaaa atggtcggtg gcggatgaga ttcctgccat cgtggaagcc ctcggccatt
    24481 gcctggataa aggggacctg gtcatcacca caggaggcct tggccccacc caggacgacc
    24541 tgacttgcga agccctggcc gtttttctga aggtcggtcg ggtattccat gctccaactt
    24601 atgaagggat cgaacaattg ttccggaaac ttggacgttt acccagcgct tcccatttag
    24661 accaggcgag acttcctgaa ggcgccggca tcctcaccaa cccggccggc actgcgccgg
    24721 gcatgcattt cgtgcacatg ggcaaacata ttatttccat gccaggggtc ccatttgaga
    24781 tgaaagccat tttcaacacc caagtcagga agctggttca acagtataaa acaggcccca
    24841 aaaggttcag ccataccttc aatacggtgg gggagggcga gaccctgctg gaagacctta
    24901 ttcatgacat caccgccggg gccccctcca atatccgttt ttcctttctt ccggatctgt
    24961 accgggtacg catccgcgtc gacctggaca gcgaaaatcc ggaagaccat gcggcctggc
    25021 tggatatcat tcaacaggta aggctgaaac ttcacagata cattgccggt gaaaatgaga
    25081 ccaccctgga actggctatt ggaaatatac tccgtgaaaa aggctggatg atgggaaccg
    25141 cagaaagttg tacgggagga atgatagggc atatgattac ccgcaacgcg ggttcctcgg
    25201 aatatttcaa gggagggatc ctggcctatt ccaacgaact gaagtcaaaa ttcctcgagg
    25261 tggaccctct aactcttgaa aaagaaggcg cggtaagcga agccaccgtc cgccagatgg
    25321 cggctggtgc gctcgacagc ctggacgtgc aggtggccgt tgccgtcacc ggcattgcag
    25381 gccccggggg aggttctgcc gaaaagccgg taggcaccgt atggatcggt gtggcggata
    25441 tccatggccg gatcctttcc aaaaaaatac aattgcggag ggaccgactg ctgaatattc
    25501 aggcagcctc caccatggct cttatcctcc ttcaccggtt cctcttgggt gaataattcg
    25561 tacttttgcc gcatatggaa catgacctga tcatgccccg gatgggagaa agcatcaccg
    25621 aggccaccat tctgcggtgg ctgctccggg aaggggatga agtggatctg gaccagcctg
    25681 ttctcgagat agcaaccgac aaagtggact cggagatccc ctccccggtg aaaggaaaaa
    25741 tcagccgggt gctttttgcg gaaaatgagg tggtgccggt tggagcaacc attgccctca
    25801 ttcaggtgga ttcggaactg aatctcccgg aaatacccac ggatgaaatc ccgatggccc
    25861 gcgaatccgg acctgttgaa acccaggttt ccgagcctgt tccctatgta cccccaatgg
    25921 taagtccgcc tgagcccgag cctgccttca ggtctgctcc caccggatcg ggccggtttt
    25981 attcacctct ggtcaggacc atcgcccggg aagaaaacat cagcctggaa gaattggata
    26041 agctggacgg cagtggacag gagggaaggg tgaccaagca ggacatcctg aattatgtgg
    26101 atcgaaagaa aaaaggcctg accgatacgg tgccaccacc ggtggttttt ccgcagccaa
    26161 ccccggtacg ccccggcccg gtaatcacgg gtgaagggtc cgggggccag tccgagatca
    26221 ttgaaatgga ccggatgcga aggctgatcg cggaccacat ggtccatagc aagcataccg
    26281 ccccccatgt cacttccttt gtggaggtgg atcttaccga actggtgcac tggagaaaca
    26341 aggtcaaatc cgaattccag aaaaagtata atcaaaacct gacctttacc ccgatcttca
    26401 tgcaagccgt ggtggaagcc atcagggcct atccgctgct taattcgtcc gtggatgggt
    26461 ataaaattat ccgtaagcag gatatccaca tcggcatggc aaccgccctg ccttccggta
    26521 atctcatcgt cccggtgatc cgtcatgcag accgcctgaa cctccagggc ctggccctgg
    26581 ccatcaatga ccttgcggcc agggccagag cgggccagct taagccggaa gaggttgccg
    26641 gcggcacctt tacactgacc aatgtcgggt ccttcggaag cctcatgggg acgccgatca
    26701 tcaaccagcc ccaggtcgcc atcctggcta ccggatccat caagaaaaaa cccgcggttt
    26761 tagaaaccgt gcacggcgat gtcattgcca tccgtcatat gatgttcatg tccatgtcct
    26821 acgaccaccg ggtgatcgat ggattcctgg gaggcagttt tttgaggaag gtggctgacc
    26881 tgcttgaaaa ttttgatatt gcccaatccg tttaatgaca gatggtcaat cccttcagag
    26941 tctccagcat gatcttattc ctgttcctgg cgtgggaaag ccgcggccaa tccatggaat
    27001 atctccgcct gcaggcggaa ggactatacc agtccaaaca atatcttcgg gctgcgcctg
    27061 ttttcaggca actccaggcg ctggcgcccc aggactatga ggtcaaactc aaaagggcgg
    27121 tttgcgaata cgaagccaat aacctggata cggcattcgc cctcgcggta agtttgatcc
    27181 ctgtacaaaa gcccgagacc cagtacgcgg gatttctcgc ggggaaaatt gcgcaaagcc
    27241 ggggggattt tgccaactca gtggtttatt acaaacaatt tctcgggcag gccctcccgg
    27301 gaagcaaact ttacctggcg gcgatcgccg aaatcaaaag atgcgggtca gccctttaca
    27361 tgaatccgga tttgcgggcc agcctggtcg aaccggccgg tgacgaagtc aataccatct
    27421 ggaatgaggt ccatcccgta cccagtgtca atcttgattc gaggatctat ttcagctcaa
    27481 accgcgaacc cgccggcttt cccctcaatt attcagccga ttacgagagc ccttacgata
    27541 tgtattccag ttccctgaac aatggggaat ggtctgaaat ttctgcttta aatccggttt
    27601 tgaacacccg ggaccatgaa ttgctttccg ggtttaatga aaacggacgg atcgtctatt
    27661 tccgccgtgg tgaaaaactt aaccggttga acttgctcgc cgatacctta agggcggaag
    27721 gaatgtcccc ggccacttcg gccatcgatt ttccacacct gggtgcggag gacgagcttt
    27781 attattttac cgattcgatc ctgatccttt caagcaaccg accgggagga cttggtgggt
    27841 ttgacctgta ttattccatt cgttccggaa gccaatggtc atccccggtg aacctgggca
    27901 tccggatcaa cagccctttt gatgaagtga gcccttcgct ggcagaagac gggcggaccc
    27961 tgtttttcag cagcaacaac ctgaacagca tggggggatt tgatgtcttc accgctcatt
    28021 ttgatgatca caccgaaacc tggtctgagc cggtcaactt cgggatgccg gtaaactccg
    28081 gggacgatga ccgttatttc cggatgttga aaaataaact gggcgccgta tttgcatcca
    28141 accgtaaaac cggcactggg ggattcgata tttatttcgc ctattttcgc cagtctccgg
    28201 ccccccaggg agaacggtca cgccccttgt tttttgccga agtccctgcg tataaatcct
    28261 cgcagcaaaa ccccggcggg agccttgatc caaaaaatcc aaacctcccg gggagcggac
    28321 aaaaggccca gttaaaggtg ccggtgatct actacctcaa cgatgaacaa ttgaagttgc
    28381 cggctatcca gaaaaaaatg gatgaagtcg ttcaattgct caaacaaaac ccggccgccc
    28441 aggtgatcat cgaggtcttt tctgaccgga gcgcagctac catttcggac gatttgatgt
    28501 attccgtgct gcgcggagaa acggtacggg actatctggt gcagggcggg gttcgaccat
    28561 ccaggatttc ggtgaaggga tatggccagt tcttcccggt ggccaaagaa aagatagacg
    28621 gcctagccaa tccggcaggc aaaagcctca accggcgcat agaattcact ttttaccacc
    28681 cggctcctga agaggccgga agtattttca atcaaaaaga cctggctgaa gtggacgcca
    28741 ggatgcaggc gcctgaaagg agccgctttc tggaattgga aaaggggttg aactataaaa
    28801 ttatgctgct ttcttccggc aatgcggcga acttccggac agatcatctg gaccaggtct
    28861 ttatagataa aaacaacctc accggcaaat atgagctgat gtccggagta tttacgacct
    28921 ttgcccttgc aaaccaggcc agaaaaaagt ggatggcttc ggggtatgaa gatgccctgg
    28981 tcgtgcctta ttactccaac cagcgtattc cccctcaact tttaaataaa tggagtgaaa
    29041 aattccctga cctgacccag tttatatacc ggggtgaata atcaatcaag aatccgtgat
    29101 gaagaaaaca aaattgttcc tggaccaaag cctcaaaaac ctgatgaccg tggggacctt
    29161 cacccgcagt tcctccttcc tgtgtaaaga gatcgtcagg caaattgaat tcacgcccgg
    29221 ccaggttatc ctggaactcg gagccgggga cggcgtgatc accaaatacc tcctggataa
    29281 aatggctccg gatgccaggc tcatcgcttt tgaggtgaat cctgtttttt gcgaagaatt
    29341 aagacgcctg gaagaccata ggctcaccgt cgtagaccag gacgccgcac ggatgcatga
    29401 gatcctggcg gccctgaaca tctccacggt ccagcatgtg gtctccgcca ttccatttgt
    29461 gatctttccg gaaacggttg ccctgcacat catccaggcc gcttttgatc atttggaacc
    29521 ttcgggcaaa tttgtccagg tacattattc cctgctggca aaaaaactgt atgacaaaat
    29581 tttcggttcc gtccggatcc ggtttgtgcc tttcaatatt cctccagcct ttctcctgat
    29641 catgcagaaa gcttccgttc ctgcaccggt acaggctctt gcccaatgag ggcgccgatt
    29701 tccggatctt ttgaccagag caggtaataa cctccgcctc ctttaccgca aagctttaca
    29761 gcatatttcc cggattccaa tcccttttgc catatccctg ccacgccggc cgtgatcagc
    29821 ggttgaaacc atttccattg caaacctgaa acctggaaaa aaaggtcctc cgccgcctgg
    29881 agatcctgct caaaccaggc ctgcaccaac ctatggttaa ggtcattcag ttcctctacc
    29941 acgggaagga acaccggctg tttcaattgc tccaggtacc aggaaatcgc atgaatatgg
    30001 cccctggcca tccctgagtc tatgacataa agaaaggaca tcaaagggcc agaagacaaa
    30061 gggtcaggta cccggaccag gctcttgccg atccaggcaa tcccctgtcc cttataggat
    30121 accaggggat caaaacctga actggtccca tggaaataat cttcaacccc tgccagttgt
    30181 ttctgcagga ttccggtgtc ttcacttatt tcccttccat aatgatagac cagggcggca
    30241 gataaggctc ccgaacttcc caggccataa ccgggaggaa tggtggagcg gaactgccag
    30301 ccttccagga tatcgtcgtg aagccttccg ctgtcgatat aatctatttt ttgaagatat
    30361 tgaaccaggg gcaggaggga ggtatcgggt tcggctcctc tttcccaaat tccggcaaat
    30421 ttgggaaagg ggatggccag ggcatccccg ccccggatga ccgtaaattc accgctgacc
    30481 agcaatttgg ccggaaaagg tctggcaggc ttcaatgaaa ataaatttga gatctaaaat
    30541 acatcattgt ttcccctggc gggctaccct gaacaggatg gcggcgagtc cggcaaaaca
    30601 gagggcgccg gtgaaatgat atccgaaggt tccgaaaatg gcttccatcc gggctccaat
    30661 gctcagtttt tccaattggt ccaggtattt aacggacagc agggcgatga tcacgcccgc
    30721 cagcgcacca ccggccacca aaccggtggc aaacagtttt cccttgctga gttcttcgtc
    30781 ttcttcgatc acgatggtat cgtgtttgga cctccagtcc acgagccctt tgaccaggcc
    30841 cccgatgaag atgggcaggg tagtgctcaa gggaaggtaa agtccaacgg caaaggagag
    30901 cgaattggtc ccgcacaatt caatgaccat ggccatgaag gccccgacca atacgaactg
    30961 ccagtccagg ttgaaagaca gcaagccttt gatcaaggtg gccagcaagg ttccctgcgg
    31021 cgccggatat ttatccgaac caatggcatg ttccactccg gtccgcagga cctcggcgtc
    31081 ctggtccagg tagcggatgg tgtagccgat tacgacggag gacaccaggg tacctataaa
    31141 taaggccagt tgctggtagc ggggagtggc ccccagcagg taccctgttt tgaggtcctg
    31201 ggaagtattg cctgcatttg cagcagcaat acagatgatg gatcccacca ccagggccat
    31261 gggttcataa gcctgtccag tccaacccat cgcgataaag atcagggcgg ttcccatgag
    31321 ggtggcgatg gtcatgccgc tgatgggatt cgaactggtg ccgatcaggc cggtaatccg
    31381 gctggagacc gtcacaaaca ggatcccaaa aaccaggacc agcaccccga tcatcagttt
    31441 ggaaaggatg ccgctgcccg ggatccccgg caggagggca atgatgacta tgagggccag
    31501 gctgccaaac aagacgactt tttgattcag gtcacggtcg gtccgtccct gaccctcccc
    31561 gtttaactcc tggttttcgc ggatggaact aaaactgttt ttaaaagagg aaatgatggt
    31621 gggaatggtc ttgatcaggg tcatgagccc gcctgcggtg accgcgcctg cgccgatttg
    31681 gcggataaaa gcacggtaga gggcaatttc agggttggcg aagacctgat tttccgggtc
    31741 ccataccccg gggcctccgg gggttttgat atccgcgagg tagcccagtt tgaccaattg
    31801 ggccgcaatg gtctctgccg ggaccagcgt ggccagtaaa ggattgagcg ccagccaggc
    31861 cagtacgccg cctgcgacca ggaccccggc aatccttggg ccgatgatgt atcccacacc
    31921 gaggtattcc ggcgtgacat ccgcgttgaa ggtggccgag ggcaaaaatt tatttttcgc
    31981 ctgggtcacg aaggtgggcg tttcggcaat gaagtgaatg atcttttgaa gaactgcata
    32041 tatggtagcc actgccaatc ccagataggc ggtccttgca aaatcaccgc ccttttcgcc
    32101 ggcgatcagg accgaagcac aagcagtgcc ttcaggataa ggaaggcgat cgtgttcctt
    32161 gatgatcagt ggccggcgca gcgggatcat aagcagggtc cccagcatcc ctccgatcat
    32221 ggccagggaa agaatggtca tataattgaa ataggatgcg ccggaaaccg actggtcttc
    32281 attggccgat aagaaaagga aggcgggcag cgtgaaagcc actccgccgg caatggcttc
    32341 cccggcagaa ccggtagttt ggatgatgtt gttttccagg atgcttgtat tgaacaaacg
    32401 cctcccgatc gaaatggcca ttaccgcgat gggaatggaa gcggagatgg taaggcccgc
    32461 cttgagcccg aggtaaaccg tggcagctcc aaagaccact ccgaaaaagg agcccagtaa
    32521 tacagacctc caggtgaatt caggcaggat ggtgggggag gggacataag gactgaattt
    32581 tttattatcc ataagtcttg ggtcttgacg gaagtggatt attgcatgcc tgtctgtccg
    32641 gatttatttg acctctccca tcagcctgcg ggtccagggc gtgaggatca ggaggatcag
    32701 tgcagaagcc gcgctgaata caaaaacata aaagaatatc tggttgaccg ggtaggtttc
    32761 aaaaaggccg ccgacccggc ccccaatcca gtttcccagg gaggcgccca taaaccagat
    32821 gcccatcatc tggccgacga tccgctccgg tgccagtttg gtcatggcgc tgagtccgac
    32881 cggggataaa aacaattctc cgacggtgtg cagaaaatag gtgcacaaca accatatgat
    32941 gccgactttt tccactccgc cggccgatat ttttgctgcc gggaccagca aaagaaagcc
    33001 caggccaaca aaaaacaatc ccagcacaaa tttgaacggg gtggaaggtt ccctcttttt
    33061 tttggcgagg gtaaaccaaa ggagggcaaa caaaggggaa agaatcagga tccacatgga
    33121 attgaccgat tgccaatacg atgaaggaaa gggagtgcca aaaagggtat tatcggtatt
    33181 gcggtcggca aacagggtga gggtcgaacc ggcctgttcg aaggctcccc agaacaaggc
    33241 cgccgccagg taaaagatga tgatcgccac aatacgtttt ttttcatccg gatcaaaccc
    33301 gccccgtgtg aagaggtaaa agaagtacac caggggtatg atgatcaggg cgctgcccat
    33361 tatgtcggag agcctccgaa tggaaagcct gtaccagcca aaccaatgga ggccggccat
    33421 cagggcaatg aaaagggtca ggtagagcag ggattttttt aataaacggg tggttgggtt
    33481 tgctactcct tcggggaccg cggtgggttt taaaccggct tcaccaagga agcgaccgcc
    33541 ccatttaaag aggatcagtc ccaaaaccat gccgatggcc acggcgacga aaccgtattg
    33601 ccatttgatg ttctgcccga aatatccggt gatcagcggg gcaaaaaaag cgccgaggtt
    33661 gatgcccata taataaatgg aaaaggcgct gtccctccgg ctgtcaccgg gcttgtacaa
    33721 tgagccgacc atggtggata cattgggttt cagcaaaccg gtgcccaccg cattgaacag
    33781 gagcccggta taaaagccgg tcttgcttgc cgtaaggccg atcagcaggt tgcccaccgc
    33841 gatgatgatg ccgccccaga acactgaatt cctggccccg aggattttat ccgatatcca
    33901 gcctccgggc agattggtca tatacacgcc ggatacgtac agggcatata aggcggctgc
    33961 ttcttccacg ctcatattca ggccggggtt ctggccggtc acggaggcgg tcatgtatag
    34021 gatcaacagg gccctccccc cataataact gaagcgttcc cacatttccg taccgaataa
    34081 tatggccagt cctttcgggt ggtttttaaa ttggggtacc acgatgatcg ccagcaaaac
    34141 aagaaataca atcagtccgt aaatcataag gtctttttat tggtttaagg tttttaccga
    34201 atggatcgga tgaaaggcag ttcatcaagc atccagagcc cgaagcagca tgggccggga
    34261 tgcggtaaaa tgacatttca tgaccccggg ttaaaaatag tcaaatccgg ttgatttgca
    34321 tccggcccca accgtcctgc cggaattccc ctacccttat ttttattaca tttgttaccg
    34381 atggatattt tcgattttat atttcacatt gaccagcatc tcgaggcctt catcgcccaa
    34441 tacgggctcc tggtttacgg gatcctcttt ctcatcgttt tttgtgaaac gggcctggtc
    34501 atcacccctt ttctgcctgg tgattccctc ttgtttgcgg cgggggccct tgccgcaaac
    34561 ccggctcatg gcctgagcat ctggaccatc atccccctgc tgatctccgc ggccctgctg
    34621 ggggacaaca ccaattattt tataggcaaa tacctttcca acaagggggt gggggccaaa
    34681 cttttcggga tctttaccat caaacaggaa tacatcgacc gcacccatgc attttatgaa
    34741 aaatacggcc agcggaccat tatcctcgcc cggtttgttc cgatcgcgag gactttcgca
    34801 ccttttgttg ccgggattgg aaaaatgacc tatggccggt ttatcaaata ttgtatcgcg
    34861 ggcgcaatcc tttgggtggt cctgctgacc ttcgccgggt attttttcgg aagcctgccc
    34921 tgggtaaaaa acaattttga aatcgtagta ctggggatca tcgcggtatc cttgctgcct
    34981 atggtcatag gctatttcag gaggaaccgg gcttgatccc tccggtcagt tgaccaggct
    35041 catggccttg tcgatgccag ccagtgcaaa ttgaagaatg ccctcggagc cccggccgaa
    35101 gacgtcttcc atttcttttt gctcatcctt cgtccaggct cccagcacat attcggattg
    35161 cctgcctttt ggaaaatcct tgccaatgcc tatcctcatc cgtggatagg ccctggtctc
    35221 cagcatttgt tcaatgcttt ttaaaccgtt gtggcccccg tcgcttccct ctttgcgcaa
    35281 gcggagtttg ccgagtggca aatgaatttc atccaggacg accaggacct gttggggaac
    35341 aagctttaat ttattcatcc agaaaagcac cgctttgcca ctcaggttca tataggtatc
    35401 gggtttgagg agaaccaggg aacggccttt gatcttcagg tcgcagcgca tgccaaaacg
    35461 atcggatgcc cagtttgctt ttaccttccc ggccaggaag tccaactgct gaaaacccac
    35521 gttgtggcgg gtatataagt aatccgggcc tggattgccc agcccgacga tgagaaactt
    35581 catgactcag caggacttgt tgtagggtgg caaaaataat cgataatcga agactgaggt
    35641 tatcacccga tggcctggtt gccttctgat gctctcttcg cctttcaccc ttcacccttc
    35701 gccttcgcct ttttcgcccc ttacccttta tgctcctttc gcccttcacc cttcgccttt
    35761 cgccctttac cttaaggttg aggttaagat taaggttgag gttaaggttg aggaccctga
    35821 ttatctttga aagtcatgtt cagggcaaaa ttgaaagaca cctggcgaat gtttcggcgg
    35881 atgaccatcc ggaaggcagt caatgccctg aaggtttacc tttccttcca tcttggcagg
    35941 tggagcggga ccgcgcttca ttggggcatg ccggtcagtt ttgcgattga acctactacg
    36001 gcctgcaacc tgcgatgccc cgaatgcccc agcggcttga gggctttcac ccgccccacc
    36061 ggaaacctga aaatggattt tttcagatcg gccctggatg cccttagtcc ttatgcctgc
    36121 aacctgacct tttacttcca gggagaaccc tatatcaatc ctcaattcct ggatatggtc
    36181 gcttatgcgg aatcccgggg gatttatacc gtcacttcga ccaatggcca cttcctggat
    36241 gataaaaatg tgaagcgaac cctggattcc ggattgtcca ggatcatcct ttccatcgat
    36301 ggcaccaccc aggaaaccta tgagcaatac cgaaaggaag gggacctgaa gacggttttg
    36361 cagggggcgg aaaggctggt gaaagcaaga cgggaatccg gactcgacca tccctatatc
    36421 atctttcagt tcctggtcac ctcggcaaac gaacaccagg tttctcaaat ccaccggctg
    36481 gcagagacga taggggtgga tgaggtgagg ctgaaaaccg cccagtttta ccatttcgaa
    36541 aatggaaatg acctgatgcc gcgggatgaa cggtatagcc gttataccag gtccggggat
    36601 ggacgttacc ggctgaaaaa cggactggag gaccagtgct ggagaatgtg gcattcctgc
    36661 gtgatcacct gggatggcaa ggtggtcccc tgctgttttg acaaagatgc gtcggtccgg
    36721 ctgggggacc tgaagctaca agccttcggc gccatctgga agggggaggc ctaccgggcg
    36781 ttcaggcaaa aactcctccg gggaaggaag tttatagata tctgtaaaaa ttgctccgaa
    36841 gggagccggg tctgggcctg aaaccggttg ggaaaaagcg gacttaaaat ttctttaatt
    36901 tttttgcctt tttttcctta actttatggg ttagaaaggg ttcagggata tttaactcca
    36961 tatctggaat tttactaatt tatagtccat tgacttagta gtatttcgat caaaaatcga
    37021 agtatggaat gggtatgcca ggaccgaatg aatgaccagg ctcatgaatg cagcattaaa
    37081 caatcctgtt ttttataaat ccatcttcca gggaaggctg gaatttggca caggaaaaaa
    37141 tatagaaaaa gcctttcagt tgtaccagat ccgggctgaa cagtattacc gaaatgaagt
    37201 catgttcaag gccgagcaaa tttttaatgt ggaatttgga tatattgaaa ttcccaggct
    37261 ggtggcccag gccaatgaaa aaacgtttac caataccctg aacctcctcg aatacattgc
    37321 ccaatttgcc gccgcgggtt ccatgggggc ctggctcatc gaccagggaa aggtattgag
    37381 gcataaaatg attgaaccgc aaagcgacaa ggttgccgtc caggaatatt tgcgcggccg
    37441 caaactggtg ggcaccaaag ggaaggaaat ggaggccatc cacgccctca acctggccat
    37501 cgaaaaatac gagaaacaca gccaggctta tgaaagaagg gggtatgtaa atttcaccct
    37561 caaaaactac aaagacgcgc tttccgactt caacaaaagc atccagtttg atgaaaataa
    37621 tgccctggcc tatttcggca gggcgcaggt gcacctctac aatggcgatg ttccgaaagc
    37681 cctcctggac ctcgggttga ctattcagaa atccctggcg ctccagatgg tgcattggca
    37741 ggcccggtat caaaaaggcg aactgcatta ccaattgaaa caaaataagg aggccgcttt
    37801 cgagttgaaa ttgttctgcg accgcaagtt tgacccgaag gacatcaact atttgaaaaa
    37861 aagggatgcc aactattttt atggacgcat tctgatggaa caggaacaat ttgacgaagc
    37921 cgcggcccgc ttcaatgccg ccctcgccgc cctggatgaa aatcccaaaa gcaccaagtt
    37981 taccaaagcg gatatcctcc ttcaacgcgg gctggccaaa aagctggcgg gtaaaaacgg
    38041 gtttatggcg gatgtcaagg aggcggctac cctgggtagt aaagaagcca aaaaaatgtt
    38101 gcaggccaaa gcataatttc ccaccctggc ccgttttcgc tccaagccca tgatccgctt
    38161 caactcaagg acggtttctt tcgtttttta ttttccgctg ctgttcgcag gctggatgtg
    38221 ccgtcctgca agccttgcgg cccaaaccca aacgaaagcg gctaagctgt ttgaacgggc
    38281 aaaatcggat atctcaaaaa acaggacctc cgaagccatc ctgaaactgg aggaagctat
    38341 tgaagaagat cccacctacc gggaagcata cctggaatta tttcgcatct ggcaatccct
    38401 tgacaaaccg gcggaggcga tttccgttct tcaaaaagcc gccctctcgg tccgctccgg
    38461 ccaggccccg ctttggtaca acctggccca actggaattg cagcaaggct ggtatgccaa
    38521 ggctcatgaa catttcacca cttatctttc gctgaatact cccgatgcag ccacccgggc
    38581 catagctgca tggcttttgt ccaagaccgg atatgccctc aaacatcgga aagacagcct
    38641 ggatttccgg tttacacctt tgaaaggaat gatcaatacc gccgatccgg aatacctgcc
    38701 ttccctggat gccacaggta aaatgatggt atttaccagg agaaccggca accaggagga
    38761 tctgtggatc agtttccttc aggccgaagc atggtccgca ccttcccctt gggcgggcaa
    38821 cagcaatcag aatgaaggcg cccatgtaat cagtgccgac gggaaaacca tcgtgtttac
    38881 cgcatgcggc agggaaggcg gattcggcgg atgcgattta tacagttcgg atttccggca
    38941 gggaaggtgg acccggccga ggaatatggg gcaggtcatc agcagccggg cctgggagag
    39001 ccagcccagc ctgaatgcca acggcaggat cctctatttc gtctccgatc gacccggcgg
    39061 gatgggagga tacgacatct ggatgagttt caaagggccg gccggatgga cccctcctgt
    39121 caatgcaggg ccaaatatca ataccgaaaa agatgaatta agcccctgtc tccatgccga
    39181 cggcctcagc ctgtatttca gatcggaggg ctggcccggc tttggctccc aggacctttt
    39241 cgtatccagg aagtccaggg aaggcaactg ggaacaaccc atcaaccttg ggtatccgct
    39301 caatgaccac agggaccagg gagcgatggc ggtgagcctc gatggaaagt cggcttatta
    39361 taccgatcag agaatcggtt catccaacca gctattggct tccgatatta tcaggcttga
    39421 actgccacag cccttccgtt ccctggcctg cacctttatc aggggcagga tttccgacct
    39481 ctcctccaat gcagccctgc cgggggccct ggtcaccctt gcggggggag aaggcctgtt
    39541 tattaaagac tcggtggtca gcgatccgga tggtgaattt ctccttatag tgccactgca
    39601 acatgcttac cagatccatg caaaactgga cggatataac ctgtattcgg agcgtttggt
    39661 tgctgaggag gcgttggccg cggggtatga aaggacccac gatatcaaac tggaacagat
    39721 cagacccggg gtggaggagc ctttgaaacc ggtcgtcctg gaaaatgtat tgtttgaaac
    39781 cgcttccttt atcctgttac ccgaatcctt taatgaactg gacggcttgt ctgaactcct
    39841 taaacaaaac ccatctttca gcctggccat ccacggacat acggacaata ctggggaccc
    39901 ggaattcaac cggacccttt ccggccggag ggcccaggcg gtctacaatt atttactcac
    39961 ccggcaaatt gaagcttccc gcatgacctg ggaaggattc ggtgaccgca ggcccatcgc
    40021 ttccaatgat tcggaggagg gtcggagcaa aaaccgccgg gttgaattta ttttgtccaa
    40081 aggaaaataa ggcattacca aacgcatcgc tcaccagcac aaaccgaaaa agcggatatg
    40141 ggaaaatcca aacaaatgat tgcagaacag gtccgcatct tggacatggg ggaccagggc
    40201 atggggattg ggaaggaccc ggaaggccgg gtgtacctgg tccgggatgc cgtcccgggc
    40261 gaccttgtgg atatagaagt aaaaaaacac cgcaaccggc tgggaaacgg gcgtccggtc
    40321 aggatgcttg aatcttcctc ctggcgtacc catccgtttt gcagccattt tggggtctgc
    40381 ggcggttgta aatggcagca catgagttat gagggccagt tgtcttacaa attcaaaaac
    40441 gtcgccgatg cattaaaacg catcggcggg ttgccggatc cacaggtgcg gccgatctgc
    40501 ggtgcacccg aagaccggta ttaccgcaac aaactggaat tcagttttgg taattcaagg
    40561 tggctcgaac cggatgaact cacccgggaa ggcgccccgg acaggaatgc ccttggtttt
    40621 caccggcccg gctcctttga gaagatcgtg gacatcaccc attgccatct tcaaccggag
    40681 cccagcaatg ccatacgcaa tgcggtgagg acctgggcca aagaaaaggg ccatactttt
    40741 tacgacgtca aaaatcacag cggtttttta cgaaacctca tcataaggac caccactcta
    40801 ggccaggtaa tggtgaccct ggtgaccgga gaagacctgc cggaagccct ggagcaaatc
    40861 aaccaattgc tgctgcttca atttccgcag atcaccactc tttatcatat ccacaatccg
    40921 aaaaaaaacg acaccatttt tgacctggac cttcatctgg tccatgggcc cggatatatc
    40981 ctcgagtcga tcgggcagtt acaatgccgc attggcccaa agtctttttt ccagaccaac
    41041 agcattcagg ccgcaaggct ttacgggttt gtggaagaat ttgccaagct taccggccat
    41101 gaaatcattt atgacctgta ttccggtacc ggcaccatcg gccttttcct ggccggccgg
    41161 tgcaggcagg tgattggagt ggaggaagtc cccgaagcgg tggaggatgc ttacctgaat
    41221 gcctccctca accagattaa aaacgtggtt tttcacacgg gggatgtgcg aaaaatgatg
    41281 atggaaggct tgtttggcag ggagggaaag ccggatttga tcgtattgga tcctccgcgg
    41341 gccggactgc accaggatat cctgcccctt ttgatcgaca gcggagcgcc caaaatcatt
    41401 tatgtgagtt gcaatcccgc cacccaggcc agggacctga aggtgctttg taccgcctat
    41461 gagcaccgga tcagccagcc ggtcgatatg tttccccaca ccgcccatat tgaaaacgta
    41521 actttgctgg agctcaaatc gtgaaccaga tgcaagtgag tacagaaaaa ttcctcgtgt
    41581 tgcaggagga attgaaagtt taccggaatg cgctcaataa ggcgttggac attatgctgg
    41641 aacagggcat caccgagtac ccgatcttta tcgcgcacca ggccaccttc catggaggca
    41701 tccccatcat caaaaggtcg gaggtgaaag gcaactggaa catcaacgtc tcttcccttg
    41761 aagaatttat ccagaaaaag atcatcagca gccgcaaggt ggcctcgttt aaacaaagct
    41821 accggcccgc caccgattat atttgtttct ttgtgttgag cgaattaggg gcccagttta
    41881 tatttttacc ccgcgtatga tcctcaggta gcggtttatg aaaggaatta ttttggccgg
    41941 tgggcttggc acacgattgt ttccccttac gctcgccgtg agcaagcagc tgatgccggt
    42001 ttatgacaag cccatgattt attatcccct gtccatcctg atgatggcca atatccggga
    42061 tgtcctgatc atctccactc gggaagacct tcccggtttt gaaaaattac tccgggacgg
    42121 ctccgacctg ggctgccatt tctcatatat ggtccagccc gaacccaacg gcctggccca
    42181 ggcttttgtc ctgggtgaat ccttcatcgg ccgcgaggcc tcctgcctga tcctggggga
    42241 caatattttt tatggcgccg gcctgggcaa tatcctcaaa agttgccagc aaccggacgg
    42301 cgggatcatc tttgcctacc aggtttcgga tcccgaacgg tatggagtcg tggaatttga
    42361 cgaaaattac aaagcccttt ccattgaaga aaaaccggaa catcccaggt caaactttgc
    42421 ggtacctggc ctttactttt atgatcattc cgtcatagaa gttgctaaaa acctgaaacc
    42481 aagcccaagg ggagaatacg aaatcacgga tgtcaataaa tattatctcg aaaggggaaa
    42541 actttatgtc aaggccatgg gaaggggttt tgcctggctc gacaccggca cgcaccagtc
    42601 tctcatgcag gccggccagt ttatccaggt gattgaggaa agacagggtt tgaaaatagg
    42661 ctgcatcgag gaggtggcct accagatgaa atttatcgac gcgtcccagc tggagaacct
    42721 cgcgcagcga tacctgaaga gcggttacgg gcagtatttg ctgggtttat tacaccataa
    42781 attcttttag cgcccgggcc catggctgaa gaattggaaa aaggaccgga ggaccacgag
    42841 gatgaacaga cccgttcggt cctgaagtca tttaaaagct caaaaatctg gttgcctatt
    42901 tttatcggcc tggcggtggt cgtgtacatg ctctctgcca attttgatcc gaccgccctc
    42961 gcatccataa aatggaatcc gcataccacc ctctggctct tcctggctgt tgccttcctg
    43021 gtcatccgac acctggctta cgccttgagg ttgtattacc tggccggaga taccattggt
    43081 ttctggaaat gcataaaatt gatcatcatc tgggaattca gttcggccgt ttcacccacc
    43141 gccctgggtg gttcggcagt cgccgtattt gtattgtcac aggaaaaaat ggggacctcc
    43201 cgcaccctgg ccgctatcat ttatacggta gtgctggaca gtttgttttt cgttgcttcc
    43261 gtgcctttac tcctgctggt gattggggcc aatgtgatca atccatccta tgtggatttt
    43321 tcctccctgg acaaggtcgc tttttcgctc ttcttttttt atggcctgat cctggtctac
    43381 ggtttgtttt ttgcctacgg acttttttac aaccccaggc aatttaaaaa aatactcgtt
    43441 ttttttacga cctggcccct gctcaaccgg tttaaagggg cagcccagaa gttgggggac
    43501 gaaatgatgg attcctctta ccagattgca caccgggaca gaaggtttca tttaaaaagt
    43561 tttttgctga cggccacagc ctgggcctgc cgtttcctga tcgtcaattg cctcatcatc
    43621 gccctgattg accccgtgag ttaccatccc tgggaccagc tcaagatcta cggcaggcag
    43681 gtgatcatgt ttgtatttat gatgttcagc ccgacgcctg gggcggcggg ttttgcggaa
    43741 gtcttttttg gcagtttcat tggcgactat attcccaaaa gcgcagcact gatcatcgcg
    43801 ctgatctggc ggttcatgac ctattatttg tacctggtcg ccggagtatt ggtgattcct
    43861 gcctggctga ccggtgtgat ccgaaaccgg aaagaagcca gggccaaagt cgcccaggaa
    43921 aaagcaagcc ggtttcagaa tcctgaatcc tgaaaacggg tggaggagat aagacttacc
    43981 tttgccggtc catgttaaac cgcagacttc aaaccttcct ggccatcatt ctgggcgccg
    44041 caatcctgat gcttgcccgg tatttttatt ttcagcccgg ggtgaaagtg ggagaagaat
    44101 gccctccttt ccaaacccgc cttgccgacg ggacggcttt cgaaatggca tctctgaagg
    44161 ggaaatatgt attgatcgat ttttggggta gttggtgcgc gccctgcaga aaagaagctc
    44221 ctgagttgaa aaaattttac gaatcctggc gccagaccgg attccgggat ggctcggggc
    44281 tggaactggt gagcattgcc atagaagagg ataggagcaa ctgggaaaag gccattcgcg
    44341 aggactcagc cacctggccg gtccatatcc tggaactgga tctgtttaat tcttccctgg
    44401 tcaaaacctt ccaggttcgg gaaatcccta ccaaaatcct gttggatccc tctcaccggg
    44461 tcgttgcggt caatcaggga tttgaggaga tggcggtctt gctgaactcc cggattcaaa
    44521 aggactgaca ttttggctca aattcttcga atggtcttat tattgcagat gcgcattaga
    44581 aaataacatt taataaatgg aacaagaagg aaaggatatc ctggaggaat cgggcgtaca
    44641 accggaagat atggcagatc ctgttgaaaa ccagccagat gttgaacaag tggccaatga
    44701 ggaggaaact acggtccggg acttgaaaga taaatacctg cgcctgttgg cagagtttga
    44761 caattataag aaaaggacca gcaaagagcg gatcgagttt gccaaatttg ccgcccagga
    44821 cctggtagtc agtctgcttc ctgtactcga cgattttgac cgggctgtca aacatggtca
    44881 ggtctcggaa ggcacttcac tcatccatca taaactggtt caggttttaa aacaaaaagg
    44941 gcttgaggaa atgcaaagtc agggacaaac ctttgatccc gccctgcatg aagccctgac
    45001 tgagatcgaa gtccctgagg atcagaaaaa taaagtaatc gaaaccctgg agaaggggta
    45061 ttatcttgca gaccggatca ttcgatttgc caaggtggtg gtcggtaaat aaagggaaat
    45121 ggcaaaacgc gacttttacg aggtgctgga catatcccgg gatgccgacg aaataacaat
    45181 taaaaaatct taccggaaaa ttgccatgca gtttcacccc gaccggaatc cgggggataa
    45241 ggctgccgag gaaaaattca aagaggctgc agaagcttat ggcattttaa gcgacccgga
    45301 taaaaaagcg cgctacgacc ggtatggtca tgccggggtg gatcccaata tggggggagg
    45361 attcagcgga ggcggcatga atatggaaga cattctccgc aactttggga atatcttcga
    45421 agacagcccc ttcgagtcct ttttcggagg cggcggaagg gcggaagcag gcggtcagcg
    45481 cggatccagc ctgcggatca gcgtcaccct tgatctccat gaaattgcca ccggggtctc
    45541 aaaaaaaata tcggtcaaaa aacaagtgac ctgcaatacc tgcggggggt ccggcgctaa
    45601 ggataaaagt tcgaaaaaaa cctgcaacac ctgccatggc agcggttatg taagacaggt
    45661 cagaaatact ttcctgggcc agatgcaaac caccaccact tgcccgcaat gccatggatc
    45721 cggccaggtt attacttcac actgtgcgac ctgcaaaggc gatgggaggg tcatgggcga
    45781 agatactttt gatatcgata ttccggcagg ggtggaagac gggatgcagt tgtccgtcag
    45841 gggcaaaggg aatgtcggcg ccaagggggg accatccggg gatttgatca ttcagatcaa
    45901 agaaaaggcc cacgaatccc ttaaacggga cgggtccaac ctggtctatg atctctatct
    45961 caattttgcc gatgcggctc tcggagcttc catcgatgta cccaccctgg atggcaaggc
    46021 cagcatcaaa gtccccgcgg gaacccaggc gggtaaggtt ttccgcctca aggataaggg
    46081 gctcccctcc gtgcagtcct accagaaagg agatttgctg gtacaggtca atatctggac
    46141 cccgaaacaa ttaaacgaag aagaaaaaga gatccttgaa aagcttaaac agctgcccaa
    46201 tttccagcct aatcccggta aagcggaaaa aggatttttt gaaaagatga aaagtttctt
    46261 ctcatgaggc cggctttgtt tataggcatt ttggcttcct tattcgtttc ctgcgatcca
    46321 ccggattata tttttgaaga aagccgttcc tttatcggac aaccctgggt ttatcccgat
    46381 tcggcggtct accgttttaa tatcaaagac acgaccagat attacaacct cctcctcgaa
    46441 ttggaccatg gccggaatta tccctacgaa aacctgtatg tccgctttga aaccgtcttt
    46501 cccgatgcca gcgtcaaatc cgatgtggtg agtatttcac tcgccgacga gaccggggcc
    46561 tggtatagtg attgccgggg caggacctgt actttttacc ttaccctgca ggaacaggcc
    46621 atcttctcca acacaggcga gtatcagatc aaagtcaaac cctggatgcg gtccgatacg
    46681 atagaagacg tgtacaggct ggctttccgg gtggaacggg gggataaacg gccgaaaatc
    46741 aaacaatagc attcttttta gagaaagcat tacttgactg aaaccatgca gatcactcca
    46801 tggcgggttt atacagggaa aattattcaa atgagcgagg attcagaaag gcctcacccg
    46861 acacctaaat attataaaag attcaggctt gtaaaaagga caatttccta atacttacaa
    46921 gcctgcatcc aattgtacta gctaaaaaaa cgtgtaaagg gtaaattgct agttcaaaca
    46981 acagcaaatt ggatttgcaa ttgatactac aaaaatcaac atattaatta gtgatttcct
    47041 aaaccggagt gtcaatttat tatagactgg tcaggaattt tatatagatt tgatatagac
    47101 aggttgattt tttaaattaa attagcctct gcgcatgttt ttacgagcca atattaacga
    47161 ccctctcacc cggcattttc ttaccaaaaa tgttgaacgt cagtttctta tcttcaaaat
    47221 aggcatagtg accttctttt tgatgctggg agtggatttt atcaattggc aaaaaggact
    47281 attgaatacc aatccttatg ttcaggccac aacgatattc cggtacctcg cccttagttt
    47341 ttttatttcg gtatacctgc tccgtaagag gtataattct tacactccca ataagttacg
    47401 atttcatgta acgctcaata tcttcctttt tgtcctcacc ctttttccga ggagcctgtt
    47461 gatcatgatg gatcggcagg tgaccggttc ctattatatt tttattttga tcctgaatgt
    47521 ggtcttactt ctggacctga ggccaaggat attacttatg ggtatatcat tgatactcat
    47581 gctattaact atccatcagg ctgccaatcc ctatgaaata ctgaaaatca gcctggctat
    47641 tgaagttagt tttgccaccg tactgagtac ttatgtgggc catctgttgt atagggccga
    47701 agccacaaac ttttatgcca agaaggacct ggaggagaaa caccttgccc ttgaacgtag
    47761 cctcattcag cttgaaaagc aaaatgagga actgaatagc agtcgcctta aggtccagga
    47821 acaaaatctg ttgttggagg aaagcaaaat taagctggaa acctacaccc aggcgctcga
    47881 acaaaaccgg gccctgctgg aggagcagaa taaggccctt gaagaaaaca aatccaggct
    47941 ccaggaatcg ctggccttct cacaaaacca gttgaccggc tatgccttgt cgatggccca
    48001 aaagaacgat ttgctgctcc agttgaaatc aagcctggaa aaaccggatg cctcccatca
    48061 tcaggcgctc cgactggtag accagggcat cggggaagat gatatctgga aacgttttca
    48121 ccatcacttt gaacagttgc atcccgaggt ggtccggaga ctggaaaacc tttatccgga
    48181 attaagcgct tcggatttgc gccttttcgc cctgctcaaa ctaaaaatta ccaccaagga
    48241 aatcgccgat ctcctgggca tctccccgga aagcgccaat acggcccggt accggcttcg
    48301 taaacgcctg ggtttgaacg aaggggagga ccttcagcag tttgtggacc ggtttgagtg
    48361 atttgcaggg atgggttcga tctcccttgc cccgcacccg cccctctttt taaattttgt
    48421 aaatcacggt aggaatcggg taggttatgc gggttcatca agcagtcgta ccatacatcg
    48481 tacatctttt tcattaaaat agtcctctac ggccacccct tcgggatcga tcgaggactc
    48541 ggccgtcagt ttccggaaga cctggccgat gctcgggata ttttccataa aatcgggaat
    48601 gaggacgctc agataatccc cctttttgat tttaaaggtt tcacaaccat attggtccgc
    48661 ctctcctgga taggcttctt ccgttgcagc ccggtaaatg atccccccgg tccggtccat
    48721 aaaggatatc ccgaaatttt tcctgccctg cgaatccttt acccggctgt gcagggattc
    48781 ccaggctgcg gagattcctt ccgggaagga gctggcctgc acataaagga tagaaatatc
    48841 gtggtcgagg tggtactttt ccataggctt gttttttcca aaattaagtt tggggaatgg
    48901 gtctgcccgg ggttgatagc ggcaataaag ggggcaggaa gcgtcaggcc ggtgtatttt
    48961 cctaaaagtc gatgctgaga ccgtgcaggg gttctgccct ccccatcagg gatttaaacc
    49021 ttttttacaa actccgactt aagccccatg gctccaaaac cttcaatcct gcaatctatg
    49081 ttgtggtccc cgtcggtgag gcggatgttt ttgaccttgg tgccggcttt gatgggtcgg
    49141 ggagagcctt tcacggggag gtctttgatc accatgacgg tatccccgtc aaggagttct
    49201 ttaccattgg aatctaaaat ctttgggttg gtgttttcag cggcagcggg ggccgcttct
    49261 tccgggttcc attcattaaa acattccggg cagatcagca aaccgccatc ggcataagtg
    49321 tattcggaac gacatttggg gcagggcgga aattcagaca ttggtataat ttctttttaa
    49381 gcggcggcaa aattacttat ttactgcgta aaaatttgat tcgttctgat tttaaaagga
    49441 aacccgaggg atctcctcct gcattctgca atacactttg caggtgcgga attattatgg
    49501 atacctttta gcctgatctc ccttccgtct tttcgtccat ctcaaaccaa agattaccac
    49561 cggggaaatc gccggtctcc cgggcatctc cccggaaatc accaatacgg cccggtaccg
    49621 gcttcgtaac cgcctgggtt taaaagaagg gggccctaat cagtttgtgg accggcttca
    49681 ctgatgtttg gtgcccgtga ggggcagcgg caactttgtg tcgcggtgtg aatgagccgc
    49741 tacgcggctt tcccgctatg gtttcccacg aagacacgaa ggacacaaag gggaaggtga
    49801 tcccttacat tttcttggtg gtcttcgctt cgctgtgtct ttgtgggcgc caacctttgt
    49861 gccgtcgtgt gaatgagccg ctacgaggct ttcccgctat ggtttcccac gaagacacga
    49921 aggacacaaa ggggaaggtg a
//
LOCUS       DAOQXT010000025        47300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_859_length_47300_cov_6.198243, whole genome shotgun sequence.
ACCESSION   DAOQXT010000025 DAOQXT010000000
VERSION     DAOQXT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 47300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47300
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_859_length_47300_cov_6.198243"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PKM27_06335"
     CDS             complement(<1..1123)
                     /locus_tag="PKM27_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06917.1"
                     /translation="MKKSLIAVLLWLYWTCMYSQSFSGHPPRLDWRKLETGEVNIFFP
                     KGLEPMADRVYQQIHRLHLSDSALGTARIKFNLILQNQSIWSNGYAGIGPFMSEYFLT
                     PPLNPYVIGSGDWPSLLAVHEYRHIQQFMNSRKGITRFLYTLMGEEAWAAAAGLALPD
                     WFLEGDAVWAETVYSNAGRGRMPAFRLEYRALVKENKYWPYIKARNGSFKHLVPNEYA
                     TGYPMVKYLNDHFGEDLWGGILEDAVNYKGLFSSFSKALRRHSGLSPSKLYTLSLEEL
                     KDERISVPDESIPYALASGKNSVENHVSPNPLPDGNMIYLHTGYNRLPFFKMVFPDGR
                     QEKLTSMGITTDEDFQITPPLISWTEVHTDPRWEQRTYSD"
     gene            complement(1247..2425)
                     /locus_tag="PKM27_06340"
     CDS             complement(1247..2425)
                     /locus_tag="PKM27_06340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNR06918.1"
                     /translation="MKDAYLVAARRSAVGKANKGGFRFFRSDDLAVEIIQGLMADVPQ
                     VDSKTVDDVIVGCANPEGEQGLQIGRQISVRALGIEVPGMTINRYCASGLEAIAIATA
                     KIRCGMAECIVAGGAESMSLVPMTGYKLAPSYRVAVNTPDYVVGMGLTAEAVAKKFRI
                     SRERQDEFSVRSHRLAAKAIESGFFKEQIIPVKVTDIRVENDQRIEKNYIMDTDEGVR
                     KDTTAEALSRLPAVFSMKGTVTAGNSSQTSDGAAFVMVMGEDRMKALGLQPIARLVSC
                     EVAGVEPIIMGIGPCAAIPKALRKAGLSLAEIQLIELNEAFAAQSLAVIQEANLNPEL
                     VNVNGGAIALGHPLGCTGAKLSIQLFSELRRRKLKYGMVTACVGGGQGIAGIYELLSA
                     "
     gene            complement(2462..4849)
                     /locus_tag="PKM27_06345"
     CDS             complement(2462..4849)
                     /locus_tag="PKM27_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNR06919.1"
                     /translation="MIKISKVAVLGSGVMGSGIACHLANCGFQVWMLDILPPDLREEE
                     KPDKTARNRIADTALRNAIKSKPSPLFHKDLVKRIRTGNLEDDLAVIRDCDWVIEAVV
                     EKLEVKKALFEKVEQNRKAGTLVSSNTSGIPIHLMAEGRSGDFRTHFLGTHFFNPPRY
                     LRLLEIIPGPDTRPEVTEFFMQFGDRFLGKRTVKCKDTPAFIANRIGVYAMSKIFQLS
                     EELGLDINTVDKLTGPAMGRPNTGTFRLADLVGLDTAVKVIEGIKQNCPQDEQIHKME
                     MPAYLKFLVDQKWYGNKSNKGFYLKSEEKDDKGKTIIYGLNLKTLEYEAPGKTNLASL
                     GVSKQTDDLSSRLKALIRMEDPGAVLIARSLAGLFAYASNRVPEISDTLYSIDDALKA
                     GFAWDLGPFEYWDAVGLEEGLRLAEKYGEQVAPWVLDMKESGHHAFYSREGKIRAYNP
                     TTKTMEVIPGREQLVNLVALSGSKPVVQNPDAVIHDIGDGVLCLEFRSKANTLGEGVV
                     RALLEGIQLAEEGRWKGLVIGNHGKNFSVGANLMLVAMMAYQQDWDELNMSVKAFQDV
                     TMRCRYSKIPVVIATQGYVFGGGCEMMMHCDAALCAAESYIGLVEAGVGLIPGGGGTK
                     EFALRASDAFFEGDVRIPTLIEKFKTIAMASVATSAEEAYGLGYLLRNRDQVVLNLDR
                     NLTEAKQKVLALAENYVMPVPREDVFVLGQQGLASLYIAAHSLKLGNYASDHDVKIAR
                     KAAWVLCGGDLSAPQHVSERYLLDIEREAFLSLCGEPKTLERIQYMLENNKPLRN"
     gene            5000..5698
                     /locus_tag="PKM27_06350"
     CDS             5000..5698
                     /locus_tag="PKM27_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR06920.1"
                     /translation="MLLSIFLQVANLPALDTAETTVGSQSLWDILMQTGILGIANVGI
                     NLVLSFVAIFIFVDRYRSLQRASAVDQNFMNNIRNSVQNGNIDAARNLCAHTDTPMAR
                     MIEKGLQRIGKPLRDIDAAIENTGNLELFRLEKNLSIMASIAGAAPMIGFFGTVTGMI
                     QAFYNMASQQNVTPATLSSGIYQALITTAVGLAVGIFAYIGYNLLVSNVEKVVFSMEQ
                     TTVEFMDLLQEPSR"
     gene            5723..6124
                     /locus_tag="PKM27_06355"
     CDS             5723..6124
                     /locus_tag="PKM27_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR06921.1"
                     /translation="MALKKRNKVSAEFSMASMTDMIFLLLIFFMLTSSIVSPNALNLK
                     LPSSTSKTVASPTLSVSITPEGKFYLNGKEMSYSILEKMVRVEVSKAGDPKNSTMTIV
                     ADKDSRVESLAQIMELAHVLKINAILATESK"
     gene            6141..7097
                     /locus_tag="PKM27_06360"
     CDS             6141..7097
                     /locus_tag="PKM27_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNR06922.1"
                     /translation="MQAILSEKERKDRQVARTTSTLVHALILLLLFLPLLKFPIPPLG
                     QEGILVSFGAPDMGQGEDLPMTQNEEMVEPEPPSAPQEEIVEETAREEAPAKPAETTP
                     PKAPDKKVITTEDPETAALKKKKKEEAERALEAERVKKAEEAKIRREDEARKKAAAEE
                     ARRVAAAEDARKKAEAEAAAKQQAEYENAKKQYGNVFGGGKGNTGKPGNQGDPNGDPN
                     ADNLKGISTGSGKVGGGLDGRGVVYEPNIQENSQRTGRVVVNVCVDKTGKVTSAKYTQ
                     RGSTTTDASLRQIAEESASRFRFTASSIDQQCGTITIDFKVK"
     gene            7163..8464
                     /locus_tag="PKM27_06365"
     CDS             7163..8464
                     /locus_tag="PKM27_06365"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNR06923.1"
                     /translation="MTKSFIQVKYKETLDLLYRKLPMFERIGPAAFKKDLSNIGALCE
                     ALGHPERRIKSIHIAGTNGKGSVSHMLASILQEAGYKTGLYVSPHYKDFRERIRINGK
                     WISKQHIVDFVEKIGPDIDRIKPSFFEISVAMAFDYFFRNRVDLAVVETGLGGRLDST
                     NILIPELSIITNISLDHQNLLGDTVEKIAFEKAGIIKKGVPCLIGEYQRKTQPVFKRV
                     ASSLGAPVRYADRHGKVIKTAAGHYQYLSKKGPQESLELLTPILGPYQEKNINTTLEA
                     IRLLNETGDWAVSHRHILEGFKNLSENAPLTGRWQWIGGHPRVLADGAHNLAGLQYLF
                     KELVKTEKGQVHCVFGTVRDKALDPVLAILPRHFTYYFVNADLPRAMNSDLLAEKAAM
                     HGLQGSSYGKVSLGLKAAKSAARGNDLVLVTGSIFVVGEVL"
     gene            complement(8620..9018)
                     /locus_tag="PKM27_06370"
     CDS             complement(8620..9018)
                     /locus_tag="PKM27_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06924.1"
                     /translation="MKKILLPALLTLGFSNFLAGQSSPALAPAGVTVAKNKMVSRPSS
                     FVMIHKEKDSPFEIYQDLNQDELVIRLKNQFDVLRFEVRDQAGKAMPVKGIQQQNEVH
                     AALSGLPNGTYRLIVDRNKEKIFTEFLLDR"
     gene            9164..9721
                     /locus_tag="PKM27_06375"
     CDS             9164..9721
                     /locus_tag="PKM27_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNR06925.1"
                     /translation="MKKIVIGISGSSGAVYADLLLKKLVSLKDQWSVAGVVASKNARI
                     NWRLELGTEMEIPSSMTLYGQEDFNAPFASGSARYDTMIICPCSMGTLGRIAHGISDD
                     LLTRSADVMLKEKRKLILVPRETPLSLIHLRNMTLLAECGAHILPAIPSFYSGPESIK
                     DLLATVVDRALDLAGFELDSKRWGD"
     gene            complement(9718..10740)
                     /locus_tag="PKM27_06380"
     CDS             complement(9718..10740)
                     /locus_tag="PKM27_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNR06926.1"
                     /translation="MQSKLPIFNKLEKLAGKKRLENIVGWAQKSSLPGFAGIPVYDIL
                     VFLYHESQREKIIHKVNSMAFSFLLALFPFLLFLFTLIPLLPVEGIKDMLFKELQSFI
                     PDQVEPFLFGTIEDVISIPRNGLLSVMSIILALYFSTNAMQTMMAGFEKAYDETFKKR
                     NFWQKRLDALKLVVLVALLVIISVVMIIFGNQIWNGLVDYFSSKQWVIENPVSGKQTN
                     WVIHFLFLQKYSTVGFEIVRWLITILMFHGIFATIFRYGPSLKKKLRVFSPGTTLATV
                     SSIIISLIFSALINNFGSFHLVYGSIGALIIILLYIQLNCFVIVAGFELNASIAVNRN
                     IKQVLD"
     gene            complement(10712..11149)
                     /locus_tag="PKM27_06385"
     CDS             complement(10712..11149)
                     /locus_tag="PKM27_06385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNR06927.1"
                     /translation="MPKIEISTEFCLRVRYAETDQMGFVYYGRFAEYFEVGRVEWIRA
                     LGLTYHELETKHRIWMPVMSMQVRYLRPARYDQELRIRTSLRALPHQDISFYSEIFNE
                     KNELLCAATIKLCFLDSLTGKRVPCPGFLQERISHAIETAHIQ"
     gene            complement(11142..12407)
                     /locus_tag="PKM27_06390"
     CDS             complement(11142..12407)
                     /locus_tag="PKM27_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNR06928.1"
                     /translation="MKRGVDIFADSEIASLKRVIVHRPDEGILRVSPKQSAELLFDDI
                     VFLPDMQAEHKTFVDVLRKLVGPEGVLETQDLLREALGVDLTAREKMLKSLIEYEELP
                     PSFAPKLGQLPDDQLAEVLITGYDAMDDRILFDPIPNFLFTRDIAVTLGEYMVITKAA
                     KVARSRENYLTRFILYQHPLFSSPRQSGKIIDLNILEDFPPSRKGEAVSMEGGDVMIL
                     NPEYVLIGISERTNLYAFHSLKSWLFSHRAVSNVVQINIPAERSFMHLDTIFTQVDHH
                     RFVAYRPIVQDGMSSYVEVHRINGDKQYYATLKEFLWNEISNKIEFTWVGGGESPSQE
                     REQWTDGCNLLAVKPGVAITYDRNAVTIKGFRDSGYRILHAPDFLRLPSAEVSRLEKT
                     IITIPSAELARARGGPHCMSCPILRMTHA"
     gene            12561..13235
                     /locus_tag="PKM27_06395"
     CDS             12561..13235
                     /locus_tag="PKM27_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06929.1"
                     /translation="MLKKIKSIFIVEEETSPVRPPASSVQADQEDKRMKEAVDIRISS
                     GKAGSPPESGSENRFLHVLAEAIEKSNQQGFDYLEFRQALVNLSKLSMDEPIRYKSAY
                     AAAQAMGVSPSQLISSAKGYLAILEGESKKFAIAEQNQRTRVVEERKNELEQISAEIR
                     SKQDAITKLQAEVEKLTKKLEEKKNEARVMSGKLEQTKVEFETAYKSLFGQISEDIQK
                     MDQYLK"
     gene            13256..14284
                     /locus_tag="PKM27_06400"
     CDS             13256..14284
                     /locus_tag="PKM27_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06930.1"
                     /translation="MDSTEFKPKSFWKRPEGVTGAIVLGGIVIGGGILLASALPLLVS
                     LVQSTLGLVVTLLVLGAIIYMVLDPKMRNLVWYMYKSTMRWITGLFVKIDPIGILKNY
                     VEDLKANLTKMNQQINRLRGQMHKLKELMLINQREIQSNLEIASKAKQSNQQNVVILK
                     SRKAGRLRESNMKYEDLYKKMEIMYRVLTKMFENSQIMQEDIADQVEVKEQERKAIHA
                     SNSAMRSAMSIIKGDKDKRAMFDQALEAVADDVSQKVGEMERFMELSDNFMKSIDLQN
                     GVFEEEGLKMLEKWESEGVSKLLGSEKASILSRANNEADVLDLEAPLTRPEKVGAHKN
                     QYDTFFED"
     gene            14349..15923
                     /locus_tag="PKM27_06405"
     CDS             14349..15923
                     /locus_tag="PKM27_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HNR06931.1"
                     /translation="MATRLTGFSRFLITLLILGLIAFAGYYLLNKTGVGAKLKDQAEQ
                     AESGQSTTTGGTSSAPSGAFKDAVKVGVVTWGGYAGGQYFNEGFKANSRSRFVQDYGF
                     PVEFKILDDVPVSREAWKNDEVNLLWCTVDALPTEIAGLSDFDPVVVFQADWSRGGDA
                     LVARRGINSVNDLRGKKIAVAEMTPSHSFLIWMLEAANLKATDVQIVKQASAIDAAQV
                     FKSQQVDAAVVWSPDDQACLQAVAGSRILQSTKSASNIIADAFIAKRSWVEQNRDKMQ
                     KLYEGWMKGAAEINANDDNKRKAAKILAENFSGFTEDDAYAAINNVRLATHGDNQNFF
                     GLNPDYKGVTGEQLYTRMTNSYKALGYAEGRIPNWRLISDPLSIRNTSLTGPDHAAEG
                     QKAFTKVTEEAGKEKEAIATKMVSITFRSGESALDENAKFIVDKEMVEIAKAFANSRI
                     RVEGNTDNVGSVALNQALSRRRAQAVVDYLVAAHGMNRNRFVVVGNGPDKPVASNDTE
                     EGKAKNRRTDFQLIGE"
     gene            15930..17027
                     /locus_tag="PKM27_06410"
     CDS             15930..17027
                     /locus_tag="PKM27_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR06932.1"
                     /translation="MDNWFKLRGTNAGTSGLLISAVGFIITIALWWVLAEIFSKQKPV
                     LEGQDEVTLFDTLGGDQKVKLWNEPGAEQAAGTRYEKVYPLLPRPDKTLLSFKPLIAQ
                     DKLVSNTLHSFWLNLQGYFWAILLAIPIGMVLSLFPLFRDLFSKQVDALRFLPLSALT
                     GLFIIWFGLGDPMKIAFLAVGILVYLIPVVMQRTSEVDETMVQTSFTMGASPWQQIKK
                     VFFPAALSSLIDDIRVLTAISWTYIIIAELLNRESGIGALIYTKARLGQTDRVFAVLI
                     VIILIGLFQDQLFKAIDRIINPFKYYKNKVDGLQDGKIGVFAWIVAIIAGILGGFSPS
                     LGILSYVAWVLAAAGTLFILWGFIKINKAVH"
     gene            17031..17831
                     /locus_tag="PKM27_06415"
     CDS             17031..17831
                     /locus_tag="PKM27_06415"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR06933.1"
                     /translation="MIEPKTPSLPDLIELRNISQSYDGGKTYIIKDLNLLVEDKPAQG
                     QFVIILGMSGCGKSTLLRYIAGLQSPTEGEVYLRGKLRQDSDHVGMVFQAYSSFPWMT
                     VKENVAMGLDFQGMEEKERNLKALEMIELVGLAGHADKFAQYPVLSGGQLQRVAIARS
                     LLANPDILLMDEPFGALDIKTRIQMQDLLLTLWNKFHSTIIFVTHDIREAVYLGDDIY
                     FMKYAPSSIVEKIHVDLPLQRDHETRRLPRYTELVYEVEDTMLRIAAG"
     gene            17901..19523
                     /locus_tag="PKM27_06420"
     CDS             17901..19523
                     /locus_tag="PKM27_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNR06934.1"
                     /translation="MKLKLLFVSLMTFAGFMAAAQSLDRPKLVVGIVVDQMRHDYLYR
                     YWDQYGEGGFKRILNEGFSCENAHYNYVPTVTGPGHASIYTGTTPAFHGLIGNEWHLR
                     NSRRGMYCAQDDSVSAVGGSEAAGKMSPKNLLANTITDVLKLYTYNRAKVIGVSVKDR
                     GAIMPAGHLADAAYWYDGNTGKFMTSTYYMNALPAWVNAFNDRNLGKQLFSQGWNTLK
                     PIGTYVDSERDDNPYEGKLNGEANAVFPRNIPFKDPTSYYTLLYTPAGNSLVTEFSKA
                     ALEAEQLGQDEVTDFLAMSYSSTDYAGHLFGLHSVEIEDMYLRLDLEMEKLLRFLDEK
                     VGHDKYVVFLSADHAVAQNPQYLKHKNLPVDYFRGWRMIDSLKKFLEKKYDPFLIDGY
                     SNGQIYLNEDRIVSRKLDRKAIAADLKKYVRAWDGVIDVVDRDDIPFLTGGNPVFPMI
                     QKGFYPPLCGDFYVIIRPGWFESSSMTGTTHGSPFTYDTHVPVLWYGWKIPAGKSYAK
                     YEITDIASTLSFLLKIPLPDAATGRVITELFD"
     gene            complement(19563..23426)
                     /locus_tag="PKM27_06425"
     CDS             complement(19563..23426)
                     /locus_tag="PKM27_06425"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNR06935.1"
                     /translation="MSQTKNCTISILLGVLFLAKFPLSGRELPVDLFKIDKVQETRWN
                     ALRQNTRVRVLEYNQDSYQQLKSSAPASFKLNLPTPDGYLPLSLSPTHLFSDGFILST
                     DQDKSLPFTPGLHYNGHIEGQPGSLVAISFFEDEVVAVINSTGGNQYTVGKSKAFRRG
                     SFVVYDASKTTAPSISCHSEALPNYQAKLKELHKPGLEQRNAAGCVNIYLEAGKSVYD
                     AHGGIQGATNFLTGLFNVVSTLYTRENMMVILSQVFIWTTSEPFSSSSSLSALYSFGS
                     YRRDNFNGDIAQFVRIKPSGGLAGIAWLDVLCDSYNQISQSGRFSYAEMESTYNNLPT
                     YSWTSLVVTHELGHNLGSPHTHSCSWQGGALDNCSTPEGTCAAGPAPVNGGTIMSYCH
                     LTTSGTNLNNGFGTQPGNLIRSRVDAATCLGPCEPIVVCATPQNLNISNLTSSSVNLS
                     WTGSESASSYTVQYRANSSTIWITATNATSSTAFNLSGLQQGTSYTWQVRANCSGGNS
                     DFAAGNFNTLTLTCMDIAPSNLTSSNNTSTSTTVRWQAVAGASSYEVQYKRSTASIWS
                     TSITATSSLFRTLINLTNGVGYDWRVRANCSNGQGPYSQGSFIAEAVSCLPPAGLEAS
                     AITKSGASLSWSAANRAFNYTIEYKKTTSPTWLLAGTTSSLSLNLTGLSESTSYHWRV
                     KTNCTSGISGFTESTFTTAGGPQATCNPPTGLASTSITSSGATLSWNAVAGATGYSLE
                     YKAKTSASWITMPVNITSASYDISGLSASTAYDWRVKTNCSGASSAFTQAAFTTIAAP
                     ACNAPSGLTNSNITQNGATFHWNAVPGAVNYTLEFKQAAASNWSVIQGAISTNSITFN
                     GLSPSTTYDWRVKTNCSGGLQSGYSQFSVTTQAPLVCGTPSGLSTSVVTANGATLSWS
                     PVSGASHYSLEYKASASPSWISLSAALNATTYNLTGLNASSNYDWRVRANCSFGSGGY
                     AQSTLSTLALPVCNTPSGLNTTNISSNSATLNWASVSGASGYQVEYKTSSDLNWTAGA
                     SLTGTSTSISGLSPSTAYLWRVKTNCSSGNSEFIQSSFTTASSATPDCPALEDNASND
                     NRTTAPSITLNAMIYGKIFPARENDYYKFTVPATGAYTAILSQLPADYDLRILNSSGT
                     TVGLSQTGGLANETINTNLITGTYYVRVYGWNTASSDAACYALLVKPSAGSLTSGIVS
                     TTGLSTTLFPNPVSQILNVEIFGLQSTAEISLRDVNGRSLQSLVTKEVHNQINVSGLA
                     PGFYFLQVQDEEKRRNTVKFLKAQY"
     gene            complement(23559..25199)
                     /locus_tag="PKM27_06430"
     CDS             complement(23559..25199)
                     /locus_tag="PKM27_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNR06936.1"
                     /translation="MKLKPLNLFSLAFILLVVGFTLHQQAGGIHRDLVLSAQKLIGLE
                     FSAAEIDSLLPDLGENLESFESLRKTALENRVLPALRFMPYPARYKPVANTTKAVFTP
                     LPANPAAREDYAFMTLHQLASLVKSRKVSSETLTRYFIQRLRTYNPALKCVIHFTEEY
                     ALAQAKKMDAELRAGKYRGLLHGIPYGAKDLLAKKGYPTTWGSVPFKDQVFDYDAAVI
                     TKLEEAGSILIAKMSVGELAWGDVWFGGMTRNPWDTTGGSSGSSAGSASAVGAGCLPF
                     AIGTETLGSIVSPSTVCGTTGLRPSFGAVPGEGCMILCPSMDKIGPITRSVEDAAIVY
                     QHIKGTFLSGPSASPVPFPYAYNKDIRKLRIGYLKQDFEKPYPFHVQDSLSLTVFTNA
                     GIELIPLELPNAPDLGIILTAEAGATFDELTRSGRDDLMVKQIRNAWPNVFRTSRFIP
                     AVEYIQANRFRTLLIEDMDLFFNQVDVYIAPSWAGRNLSVTNYTGHPCVVVPNGFRNG
                     RPTSISFTGKLFHESEVLAVAKYYQDKTEWHQKHPTGF"
     gene            25277..26428
                     /locus_tag="PKM27_06435"
     CDS             25277..26428
                     /locus_tag="PKM27_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR06937.1"
                     /translation="MLNCQKEKFFIAQDVTYLNGAYMSPQLRQVEEVGIQAVQRKNKP
                     WTITPADFFKEVEALKLLFARLVHTDQPERIAVIPSVSYGIANAARNISFEKGDHILI
                     LEKQFPSNYYEWVDLARKKELVLDVVKAPDSLVDRGQAWNQKVMENIKPRTRVVAMEN
                     VHWTDGTWFDVAQISKKIKAQGGFFIVDATQSLGIFDFDQNLVQADAVIATGYKWLLG
                     PYAIGCAYYGPAFDQGEPLEENWINKQYSQEFERILDYEESYRPGAARYGMGEQSQFV
                     QVPMLCASLSQILEWGVAEMQAYCRGLVDQVLPLLDQKDLWIEKKDWRASHLFGLRTA
                     GGFSPAVKQKLLDQKIFVSYRDDAIRVSPNVYNTKEDLIKLIETLRSAH"
     gene            26515..28191
                     /locus_tag="PKM27_06440"
     CDS             26515..28191
                     /locus_tag="PKM27_06440"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HNR06938.1"
                     /translation="MPAKIFLLSLLAFTSCAPKQMEVDGLITHALIYDGTGSPPVAGS
                     LAWNADTIVGMGEVGMIRAKKIIDAGGMALAPGFVNMLSWAPETLIHDGKSLSDLKQG
                     VTLEVFGEGTSMGPINEAMRQDMLASMGDHPYEIGWTTLGGYLDFLEQRGVSCNFASF
                     IGAATPRIHELGYANRQATPDEMNRMKQLVAQAMEEGAMGVASALIYAPGTYANTQEL
                     IELARTAAGFGGMYISHMRSEGNHIWSALHELFTISREAGIRAEIYHLKLSGRNNWDK
                     QDLLEQKIDSANQAGLKITADMYNYTAGATGLDASMPTWCQEGGYKAWAGRLKDPALR
                     KRILHEMETDTLGSWENFYKMCGPENMITVDFMNKDLRKYVGKTIAEISKDRGSSPAE
                     AIADLVVEDGSRVGVVYFMMSEENVRRNIRRPYVSFCSDAGSIAAEGMFLENNVHPRT
                     YGSFARLLGKYVRDEKLIPLEEAIRKLTSLPCENLKVNKRGILKPGYFADLVVFDPET
                     IQDHATYEKPHQYATGVHQVIVNGIQVIKDGEHTGAKPGRAVRGPGWKGK"
     gene            complement(29014..31389)
                     /locus_tag="PKM27_06445"
     CDS             complement(29014..31389)
                     /locus_tag="PKM27_06445"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNR06939.1"
                     /translation="MKNLFFSLWIALIGSTSLPAQEIRDFPGYFPFTYSEKDGRVTLS
                     VSQVGEEFLYVQGLSSGVGSNDLGLDRGQIGRERIVKFKRAANKLLLVQPNYSYRAIS
                     NNEAERKSVEEAFAQSVLWGFPIEREENGILYIDLTPFLMQDAHDVSGRLESARQGTY
                     RLDLGRSALNLDRTRNFPKNTEWDVLLTFTGKATGQYIRSVTPSPEAVSVHQHHSFVE
                     LPDDGYQPRTFDPRSGFFETSFYNYATPISEPLKKMLTVRHRLEKKDPFAPFSEPVKP
                     IIYYVDAGCPEPIKSALIEGASWWNEAFETAGFINAFQVREMPADADPMDVRYNVIQW
                     VHRSTRGWSYGNTVTDPRTGEIIKGHVSLGSLRVRQDYLIARGLASPFGHGDENTGEL
                     TAMALARLRQLSAHEVGHTLGLSHNYASTFNGRASVMDYPHPVVDFKDGKPDFSNAYD
                     VGIGAWDKRAILWGYSDFPQGTDELKALDQIMNETIRLNLKYLTDQDSRPAGSAHPYS
                     HLWDNGQSAVDELQRILQVRRQALQQFGLNTLRTGEPMATLENVLVPLYLSHRYQVEA
                     VSKWIGGMEYNYAVKGDSQLITRVVPSSQQALALNAVLKSISPSELSLPENILNLIPP
                     QPPGYDRDRENFKTYTGLPFDPLAAAESISGFALGFLVQPERLARIVEQNARNASHWN
                     LLSYLQNIHRGISSHPVTNGLEQEIKLNNERIFINKLLSVMADKNLASSVMSPCLRFI
                     KLTESQLSSSQSSQAGYIMEMIRRYREDPSSFEAPEPSRMPDGAPIGCFQE"
     gene            complement(32354..33568)
                     /locus_tag="PKM27_06450"
     CDS             complement(32354..33568)
                     /locus_tag="PKM27_06450"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNR06940.1"
                     /translation="MATFQSLNFSNKKALVRVDFNVPLDKSFKITDDTRIRAALPTIN
                     HILGHGGAVILFSHLGRPEAKLKPDGSIDKEKFTLRHLVPHLSELLGRPVQFCDELRG
                     NKLKSVAGALQAGEVLLMENTRFEAGEEKCDPGLSEEWASLADLYVNDAFGTAHRAHA
                     SNVGVSNCFDTSRKSFGFLMAAELTNARKVTDHPQHPFTAVLGGAKVSDKILLIERIL
                     DIADQIIIGGGMAYTFIKALGGETGKSLVEAEKIELAKTLLDKAQAKNKLLLLPQDSV
                     IADSFSDSSNTQVCNSHEIPADWMGLDIGPVAREQFRTVLLQAKTILWNGPMGVFEMP
                     SFARGTAFVAQAVADATREGAYSLIGGGDSVAAINQAGLDDEVSYVSTGGGAMLELLE
                     GKQLPGVAAIEG"
     gene            complement(33659..34669)
                     /gene="gap"
                     /locus_tag="PKM27_06455"
     CDS             complement(33659..34669)
                     /gene="gap"
                     /locus_tag="PKM27_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016365427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNR06941.1"
                     /translation="MSTVKVAINGFGRIGRLVYRQIYDMKGIDVVAVNDLTSPAVLAH
                     LLKYDTAQGRFHAEVSAAESSIVVNGDIIKVYAQKDPSQIPWKDHGVDVVLECTGFFT
                     DKDKASAHITAGARKVVISAPATGDLKTIVYNVNHHLLDGSETIISCASCTTNCLAPM
                     AQVLEEKYGIVNGLMTTIHAYTNDQNTQDAPHPKGDLRRARAAAQNIVPNSTGAAKAI
                     GLVLPSLKGKLDGTAQRVPTLTGSLTELTCILKTKVSGTEEVNNAMKAAANESYGYTN
                     DQIVSSDVIGMTFGSLFDATQTKVQNVGDTQLVRTVAWYDNEMSYVSQLVRTLKYFAE
                     MI"
     gene            complement(35043..35936)
                     /locus_tag="PKM27_06460"
     CDS             complement(35043..35936)
                     /locus_tag="PKM27_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNR06942.1"
                     /translation="MIEIRHFQLIKMISEAGSMTKASEKLFLTQPTLSHQLKEIESKL
                     GASLFLRINKKLILTEEGKKIIEAAHEILPRIAQIENDIKGIRRKVKHLRLTTQCYTC
                     YHWLPEIMKKFQTEFPTTEIDIVTEAMSNPVDYLLKGEIDLAITNNLKRQSGIKFEKL
                     FEDEQVALVPSRHPLAARPYLRPSDFKNESLIIYKEDINKDYFAQNVLIPRSIPIGHV
                     TKMQLTEARVELVKAGMGLTVLSKWLVKPFVRDTSLIKLIPIGKNGFYRTWYTGTLQS
                     RQNEPGLKQFISFLRDQELGS"
     gene            36023..38446
                     /locus_tag="PKM27_06465"
     CDS             36023..38446
                     /locus_tag="PKM27_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR06943.1"
                     /translation="MIAFLLKSSIRLIRRHQFLAILKLSGLAIGMATFLITALFVIHE
                     LTFDRQHPDFRRIFRYVHRVSSDNNLQQFAFTSATTGPALIERFQEVESFVRIFKSVV
                     SLKRNDADVGFIEKKFAFADTNFLEIFSFPLRQGYDKDDILKDPYALLLTPDMAKKYF
                     GQQDPIGKTLVLNGQIELTVKGVFRENFSRSHLNFDFVSSFSTLDIIKNNPIVSIQIP
                     ASLNLENKGFAAFYTYLKLHSSDQASTLIGKFPAFIEEFRGKGRSERLKPTLQSLESI
                     HLHSDMLYEIDKNGSLKMILIYGIVGLLILAAAIINYVNISMAEFTMRSKGVGLKKIL
                     GISRSSLWLSHFMETLVLCFGGLLSGGLLAMLLIPGFNTLMETRISFFTFEMVILAGL
                     IYVVTSILSGSLPAFQMGGQKALVAFKGSFQSTKTGLIIRNALVFIQLLVSFVLLTIS
                     MLIFLQIDYLLQKDPGFASDQIIVVNASSLSLDERRSIKTTLGTDKNVVSTSMCSTPP
                     GEPLFTFGATLPGNSGDEDRRLTFYQMFVDEDFLSAMGLSLKEGRFFDGRISADSVNS
                     FVINEAGAKAIGHQAISQRIEIPSMIPGVRIKKNVVGVIHDFHFSSFHSAVEPLLLEF
                     NPQNAGYLMVRFNPVQAKNVIAAIEQAWKASAARLPFNYYFLDDGFARYYAAEQRTKK
                     IVALVTLLTVFLASLGIFGTSLFTMQQRSKEMSLRKLLGSTAINLFALVFRPFLLGLV
                     WASLVGAPMAVWIGNEWLRNYPYHTSFSYLLPVISFGLIAIIIFITLLFYLFRIIKMQ
                     PAVVLRESN"
     gene            complement(38614..39888)
                     /locus_tag="PKM27_06470"
     CDS             complement(38614..39888)
                     /locus_tag="PKM27_06470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR06944.1"
                     /translation="MRKPAITTACIFLWAALVIAFFISWEILKKPPVVKGDLVSIENY
                     VVHRLKNAQADKKLGSAALVLVQNGRIITEQGFGVAHFETNTPVNTEETLYLLSSLSK
                     AVTAWGIMKLVQEKKIGLDEPVLPFLTRWQFPGSENYRQRVTVRHLLNHTAGFEDNYG
                     HGGFLPGEELQTIEESLTFPKDVNMGQPHAALIVREPGTVMSYSSAGYAVLQLLIEEI
                     SGQAFNDYMQEKVFLPLKMTKSCYSLDSILAQGRKHHLASNYDLNLKTYPHRRYTNMA
                     GVSLRSTAHDLAQLLRAYYNTNAVLTKESIKEFGTPQPGTSLTWGLGHTLYGENNSGG
                     YVFGHGGGAFPASGAEMRVNPATGNGIVLLASGTQGLITEISDVWTYWETGNKSFDIR
                     NVVNNWLTHAIIAIIFGALVIVSLRNRKMVNP"
     gene            40646..42718
                     /locus_tag="PKM27_06475"
     CDS             40646..42718
                     /locus_tag="PKM27_06475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNR06945.1"
                     /translation="MQGKQLRKLEAELWRAADQLRANSKLTASEYSMPVLGLIFLRHA
                     FNRYQKVKTEVEKDLPSHPQRGKRPLTKKDFEARNSMFLPEKAQFDYLVSLPESADIG
                     DAIDQAMKSIEDEYDNLKGVLPKNFSIFSKDLLKELLRIFNKEVLQKAEGDLFGKIYE
                     YFLGKFAMTGAQEGGEFFTPMSLVNTIVNVIEPDHGIVFDPACGSAGMFVQTGYFIES
                     EGLRPAEKVSFYGQEKAELNTKLAKMNLAVHGLEGNIQEGNTFYEDKHNLVHRADFVM
                     ANPPFNVDGVDKAKEIVKKDPRLMLDGKVYLPKNDNANYLWITYFYNYLKPTGRAGFV
                     MASSASDAGHSEKEIREKLVKTGAVDVMMAIGNNFFYTRSLPCTLWFFDRAKEKDKEK
                     KDKVLMLDARKIYRKVTSKVNDFSEEQLQNLICIVNLYRGNTQKLKSTIKYYLETAAA
                     LAKETADATMELQKQLQLVHKTVSGFATKYARENKEAKNFVDSLKFEETASFYEQQDK
                     LIDAIINVKADITSLENIAHQCKTLRKPQDKLIKQLLDVISSAVKEFQLNKNKDWKEL
                     NLKEQVDQLKALQQQLSGNPDEEEPGLLHETEYFWKQAYWLQSRFPDGVYTDVEGLCK
                     IVSQKEIEANDWSLSPGRYVGVDNATDDDFDYEERLNEIHIELEGLNEEAIALSNAIA
                     ENFKQIVL"
     gene            42715..43863
                     /locus_tag="PKM27_06480"
     CDS             42715..43863
                     /locus_tag="PKM27_06480"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNR06946.1"
                     /translation="MSYRIEKMKDAPLKFIDGDRSSKYPKRNEFQSEGIMFLNANSIS
                     SGIFDISAVNYISQEKYETITKGRIVPNDIILTTRGNGVGDSFLFKEKYGEGLINAQM
                     LIIRCDEKMLSPQYLYYFFTNPACKNYFINCSSGSAQPQLPIKSLKEIEIKYPPLPTQ
                     RRIASILSAYDDLIENNLKRIKLLEEKAFLRYKGIVRNEKMEELTIEDLANVVMGQSP
                     ISDTFTDEKNDIPFHQGVTNFTNYFVEHKIYCNSPIKIAEAGDILFSVRAPVGRINFT
                     TDKICIGRGLCAFRNKKGFQNFFYHQLKEFFFTEDVIGNGAIFNAVTKLELMKVKFKT
                     PSEKIQLEFENEAKVIYNLILNLTMQNAKLMEARDILLPRLMNGEIEV"
     gene            43958..44494
                     /locus_tag="PKM27_06485"
     CDS             43958..44494
                     /locus_tag="PKM27_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06947.1"
                     /translation="MNRFSLEIWDDEGRICTFYTVRWVTVNDDTSSETDRFFDTYAMP
                     THPLEERAMQLFRLITESIGNRYGATDDFFDRIVNKAQELPPKPKQWVEEIKDLGINF
                     PLRLFCYRVTENIVILFNGGIKESQATQKSKDLSMKFYEAQTFVKKIEEALRTKMIEI
                     SDDERHLQNFDGTTDIIF"
     gene            44499..44912
                     /locus_tag="PKM27_06490"
     CDS             44499..44912
                     /locus_tag="PKM27_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR06948.1"
                     /translation="MRSKVVDRLLKSIPKDVEIFVDWYADLVVRINQLLHEKNISKKE
                     LAEKLDKNPSEISKWLSGEHNFTLRSLAKLSAELGEPLLEVPKKKAQTEFVEGGFICR
                     VHTFVSYTKIDAKTTDKVWQLAEEIEPYKALSNAG"
     gene            44902..45354
                     /locus_tag="PKM27_06495"
     CDS             44902..45354
                     /locus_tag="PKM27_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06949.1"
                     /translation="MPDKNIFDPEKITLIDFKMIKGQVDTPENFDNSKVSGHQLDNSL
                     KLAFNLVDKLAKVDFTLTIKTDSKGENESEATGNFHLIFIYRIENLAELAIPEKNKRL
                     NLDAGLANALSSVTYSTSRGILLTRLQGTALQDFVLPIINPNKLLQNK"
     gene            45361..>47300
                     /locus_tag="PKM27_06500"
     CDS             45361..>47300
                     /locus_tag="PKM27_06500"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNR06950.1"
                     /translation="MTWEYSEDNLIEKTAIDLFFNQLGWDTAIAFNKETFGEGSTLGR
                     LNKKEVILKRIFFDRLRDLNPGLPDLAYQQAYEKLSTDSVTRSLAEINYEKYQSLREG
                     IPVDFVNDRGEQVKNKTLAVFDFDKPETNNFLAVRQLWVQGKSNRERRPDIIGFVNGI
                     PLLFIELKAAHRKLENAFNDNFTDYKDVIPNLFYYNAFVVLSNGIESRVGSITGKYQH
                     FHEWKRISEEDEGVVALDRIIKGICNKKRFMDLFENFILYDHSLGKVVKLIARNHQFI
                     GVNKAIENIKQKILLYAEGKISLEEKQKLGVFWHTQGSGKSYSMVFFGQKIHRKFTGS
                     YTFLIVTDRNELDNQIYGTFSGVGVIPQVKAGSRDSLKAGSGQHLRELLGSDHRYLFT
                     LIHKFNFEDVITPRDNIIVISDEAHRTQGGTLAMNLRNALPNASFIGFTGTPLFKDDE
                     LTKRIFGDYVSKYDFQRSVDDGATVPLYYENRGEYLGLKNPAINEQIRAVLDQETEDL
                     DSDQRSKIEQLFAREYPILTTRKRLEAIAKDVVWHFCNRGYKGKGMFIALDKLTAVRM
                     YDLILHHWKLYVEQLEKEISKANYGDQEYQDKSRELKWIKETEICVVVSGEQNEIQKF
                     RAWDLDIEAHREKMNTQDLETR"
BASE COUNT        12268 a        11195 c        12126 g        11711 t
ORIGIN      
        1 catcggaata agtccgctgt tcccacctcg gatccgtatg gacttcagtc caggagatca
       61 ggggaggggt gatctggaag tcctcgtcgg tggtgatgcc catggaggtc agtttttcct
      121 gacggccatc cgggaatacc attttaaaaa acggaaggcg gttgtacccg gtatggaggt
      181 agatcatgtt gccatcgggc aggggattgg gtgacacgtg attttcaacg ctgtttttgc
      241 ccgaagccag cgcgtaaggg atggactcgt ccggtacact gattcgttca tctttcaatt
      301 cctccaggga aagggtatat aattttgaag ggctcaatcc gctgtgcctg cgcagagctt
      361 tggaaaaaga ggaaaacagg cctttatagt tcaccgcgtc ttcgaggatc ccgccccaga
      421 ggtcttcccc gaaatggtcg ttgaggtatt tgaccatggg atagcctgtg gcatattcgt
      481 ttgggaccaa gtgtttgaag gagccgttcc gggctttgat atagggccag tacttgtttt
      541 cttttaccaa ggcacggtat tccaacctga acgcaggcat acgaccccgg cctgcgttgg
      601 aatacaccgt ttccgcccaa acagcatccc cttccaaaaa ccagtccggc aaggcaaggc
      661 ccgccgcggc cgcccaggct tcctccccca tgagggtata taaaaaacgg gtaatgccct
      721 ttctggaatt cataaactgc tggatatggc ggtattcatg gacagccagt aaacttggcc
      781 agtccccgga gccgatcaca taaggattga ggggaggggt gagaaaatat tcggacataa
      841 agggacctat accggcgtaa ccgttggacc atatggactg attctgaaga atgaggttga
      901 attttatccg ggctgtgccg agcgcagaat cggaaaggtg tagacgatgg atctgttggt
      961 atacacggtc tgccatcggt tccagccctt ttggaaaaaa aatgttgact tccccggttt
     1021 ccagctttcg ccaatccagt cggggaggat ggcctgaaaa cgattggctg tacatacagg
     1081 tccagtacaa ccacagcaat acggcaatca ggcttttttt cacgcctaaa aaataagtaa
     1141 attttctaat cggggatttt aacccatgga gtcaggggat ccattcgggc ctggattgcg
     1201 atgcatcacg ggacgaccgt tgcattatat tatttatccg gatcgcttaa gcgctcagca
     1261 gttcatagat gccggcaata ccctgtcccc ctcccacaca ggccgtgacc atgccgtatt
     1321 tgagttttct tcttctcaac tcactaaaca actggatgga cagtttggcc ccggtacaac
     1381 ccaatgggtg gcccagggca atcgctcccc cgtttacatt cacgagttca ggattgagat
     1441 ttgcttcctg tatgactgcc agggactggg cggcaaaagc ttcattaagt tcaatcaact
     1501 ggatttccgc caggctaagg cccgctttgc gaagcgcttt gggaatggcg gcacagggcc
     1561 cgatccccat gatgatcggt tccacacccg caacctcaca ggagaccagg cgggcaatgg
     1621 gctggaggcc gagcgctttc atccggtctt cccccatgac catcacaaag gccgcaccat
     1681 ccgaggtttg ggaggaattg ccagccgtga ccgtaccctt catgctgaaa accgcaggca
     1741 agcgggagag ggcttcggca gtcgtatctt tcctcacccc ttcatcggta tccatgatgt
     1801 aatttttttc tatcctctga tcattctcga cccggatatc cgtaaccttt acgggaatga
     1861 tctgttcctt aaaaaatccg gattcgatcg cctttgcggc gagacggtgc gaccgtacag
     1921 aaaactcatc ctgcctttcc cggctgatcc taaacttttt ggctaccgcc tccgcggtca
     1981 ggcccatacc gaccacgtaa tccggggtgt tgacggccac ccggtaactc ggcgccagtt
     2041 tatagccggt catgggtacc aggctcatgc tttcggctcc gccggccacg atgcattccg
     2101 ccatgccgca tcggatcttg gccgtggcta tggcaatggc ttcaaggccg ctggcacaat
     2161 accggttgat ggtcatgccc ggcacctcga tacccagcgc acgcacggaa atctgacggc
     2221 cgatctgcaa gccttgttcc ccttccggat tggcacaacc gacgatcaca tcgtcgacgg
     2281 tttttgagtc cacctggggt acatcggcca tcaggccctg aatgatttca acggcgaggt
     2341 cgtccgagcg aaaaaagcga aaacctcctt tgttcgcttt accgacggcg cttcttctgg
     2401 cggctacgag ataggcatct ttcatggtca aggaatgtga atgggttaat gatcaataag
     2461 ttcagtttct cagcggtttg ttgttttcca acatgtactg gatgcgttcg agcgtttttg
     2521 gctcgccgca aagactgagg aaggcttctc tttcaatgtc cagcaaatag cgttcgctga
     2581 cgtgttgcgg ggcgctgagg tcccctccgc acaggaccca ggcggctttc ctggcaattt
     2641 ttacgtcatg gtcgctggca taattaccca gcttgaggct gtgggcggcg atatacaatg
     2701 aagccagacc ctgttgtccg agtacaaaaa catcctcccg gggaacgggc attacataat
     2761 tttccgccag ggcaagcact ttttgtttcg cctcggtcag gttccgatcg aggttcagga
     2821 ccacctgatc cctgttgcgt aaaaggtaac ccaggccata cgcttcttcg gcagacgtag
     2881 ccaccgaggc catggcgatg gttttaaatt tctctatcaa cgttgggatt cgcacgtctc
     2941 cttcaaaaaa agcatcggaa gccctcaggg caaattcttt ggtaccccct cctcccggaa
     3001 tcaaaccgac ccctgcttcc accaggccaa tataggattc ggccgcgcag agggcggcat
     3061 cgcaatgcat catcatttca caacctccgc caaacacata accctgggtt gcgatgacca
     3121 cgggaatttt tgaatatcgg caacgcatcg tcacatcctg gaaagccttc acggacatat
     3181 tcaactcgtc ccaatcctgc tggtaagcca tcatggcaac cagcatcagg tttgcaccca
     3241 cactgaagtt ttttccatga ttcccgatga ccagtccttt ccacctcccc tcttcggcca
     3301 gttgaatccc ttccagcaag gcccggacca ccccttctcc gagggtattg gccttgcttc
     3361 tgaattccag gcaaaggaca ccgtccccga tatcatggat caccgcatcg ggattttgaa
     3421 ctaccggttt ggaaccggaa agggctacca aattaaccag ctgctcacgt ccggggatga
     3481 cctccatggt tttggtcgtt gggttgtaag cccggatctt gccctcccgg gaataaaaag
     3541 cgtgatgtcc cgattctttc atgtccaaaa cccagggggc cacctgctcg ccgtattttt
     3601 ccgccagcct aagcccctcc tccagaccca ctgcatccca atattcaaag ggcccgaggt
     3661 cccaggcaaa gcctgctttc agggcgtcgt caatgctgta caaggtatcg ctgatctccg
     3721 ggaccctgtt ggaggcataa gcgaaaaggc cggccaggga gcgggcgatc aaaacggctc
     3781 ccgggtcctc catccggatc agggctttca gccgcgagga caggtcatcg gtctgcttgg
     3841 ataccccaag gctggcaagg tttgttttgc caggggcttc gtattcgagg gttttgaggt
     3901 tcaagccata aataatggtt ttccctttgt cgtctttttc ctcggatttt aaataaaaac
     3961 ccttgtttga tttattgccg taccatttct gatccacgag gaatttgaga taagccggca
     4021 tttccatttt gtggatctgt tcgtcctggg gacagttttg ctttatgcct tctatgactt
     4081 tcaccgccgt atccagaccc accaggtcgg caagccgaaa ggtacccgta tttgggcggc
     4141 ccatggcggg gccggtaagt ttatccacgg tattgatgtc aaggcccagt tcttcggaca
     4201 attgaaatat tttggacatg gcatagaccc cgatccggtt ggcgataaag gcgggcgtgt
     4261 ctttgcattt tacggttctt ttgcccagga agcggtcgcc aaattgcata aaaaactccg
     4321 tcacttcggg ccgggtgtcg ggaccgggta tgatttccag cagtcgcagg tacctgggtg
     4381 ggttaaaaaa atgggtgccc aggaaatgcg ttctgaagtc tccgctgcgg ccttcggcca
     4441 tgaggtggat gggtattccc gaggtattgc tgctgaccag ggtgcccgct ttgcggtttt
     4501 gttccacttt ttcgaaaagg gcttttttga cttccagttt ttcgaccacc gcttctatga
     4561 cccagtcgca atcgcggatc acagccagat cgtcttctaa gttgccggtc cggatccgtt
     4621 tgaccaggtc tttgtgaaag aggggagagg gcttggattt tatggcattc cggagcgctg
     4681 tgtctgcgat ccggttccgg gccgtcttgt ccggtttttc ttcttcccgg agatccggag
     4741 gcagaatgtc cagcatccag acctgaaaac cgcaattggc caggtggcag gcaatcccgc
     4801 tgcccatcac cccggagccc aggaccgcca ctttgctaat tttaatcata cccaagtatt
     4861 tgactcaaag ttaagtttat cctggttcct tatcaaatta caatttatta taatatatct
     4921 taatgaacca ctaattttgc acgaatccat aatagttaac gggttcaatc cgttcagcat
     4981 taacttctaa aattaactta tgttattatc gattttcctg caagtggcaa atttaccggc
     5041 cctggatacc gctgaaacga cggtgggcag ccagagccta tgggatatcc tgatgcagac
     5101 gggtatattg gggattgcca atgtaggcat caacctggtc ctgtcctttg tggccatatt
     5161 catatttgtg gaccgttatc ggtctcttca aagggccagc gcggtggacc agaattttat
     5221 gaacaacatc cgcaacagcg tccagaacgg caatatcgac gcggcccgca acctttgcgc
     5281 acatacggat acgcccatgg cccgtatgat cgaaaaagga cttcagcgca ttggaaaacc
     5341 tttgcgcgac attgatgcgg ctattgagaa tacgggcaac ctcgaattgt tcaggctgga
     5401 aaaaaacctg tccatcatgg ccagtatagc cggggcggcc cccatgattg gctttttcgg
     5461 gacggttacc gggatgatcc aggcttttta taatatggcc agccagcaaa acgtaactcc
     5521 ggccactctc tccagcggca tctaccaggc cctgatcacc accgcggtcg gtttggccgt
     5581 gggcatcttt gcctacatcg gctacaacct gctggtgtcc aacgtggaaa aggtggtctt
     5641 cagcatggag cagacgaccg tcgaatttat ggacctgttg caggagcctt cgaggtaacc
     5701 attttaacaa agggaccaga ctatggcatt aaaaaaaaga aataaagtca gcgccgaatt
     5761 cagcatggca tccatgacgg atatgatctt tttgttgctg atctttttta tgctgacctc
     5821 atcgattgtc agcccaaacg cacttaacct caagctgcct tcttccactt ccaagaccgt
     5881 agcttccccg acgctctcgg tgtccatcac tcccgaagga aaattttacc tgaacggcaa
     5941 ggaaatgtcc tattccattt tggaaaaaat ggtccgggtg gaagtcagca aggcggggga
     6001 tcccaaaaat tcgacgatga ccatagtagc ggataaggat tccagggtgg agagcctggc
     6061 ccagatcatg gaactggccc atgtgcttaa aatcaatgcc atactggcca cggaatccaa
     6121 atgaaggtaa atttgtaagc atgcaagcca ttttaagcga aaaggaaagg aaggaccgcc
     6181 aggttgcacg gacgaccagc acgctggtgc atgccctgat cctgctgttg ctgttccttc
     6241 cgctcttgaa gtttccgatc cctccgctcg gacaggaagg cattttggtg agttttgggg
     6301 cgcccgacat ggggcagggc gaggaccttc ctatgaccca gaacgaagaa atggtcgaac
     6361 cggagccgcc cagcgcccca caggaagaaa tcgtggaaga aaccgccagg gaagaagcac
     6421 cggctaaacc tgccgaaacc actccaccca aagcaccgga taaaaaagtc attaccaccg
     6481 aagatcccga aactgccgcc ctgaagaaaa agaaaaaaga agaagccgaa cgggctcttg
     6541 aggcggaacg ggtaaaaaag gccgaggagg ccaaaatccg cagggaggat gaggccagga
     6601 aaaaagccgc tgccgaggaa gcccggcggg tagcggcggc cgaagatgca aggaaaaaag
     6661 cagaggccga agccgcggct aaacaacagg cagaatacga aaacgccaaa aaacaatatg
     6721 gcaatgtatt tggcggagga aagggcaata ccgggaaacc cgggaaccag ggagacccga
     6781 acggagatcc caatgcggac aacctcaagg gaatcagtac cggttccggc aaggtgggcg
     6841 gcggcctgga tggaagggga gtggtctatg aaccgaatat ccaggaaaac tcccagagga
     6901 cgggccgggt ggtcgtcaat gtctgcgtgg ataagaccgg aaaggtcacc agcgccaaat
     6961 atacccaaag gggatccact acgaccgacg cttccctgcg ccaaatcgct gaagaaagtg
     7021 cctcgcgctt caggttcaca gcgtccagca tcgatcagca atgcggcacc atcaccatag
     7081 acttcaaggt caaataatag gcccggaacc ggcaacaccc cggcctcccg gcgtgaaggg
     7141 gttgtgctaa caagaaggaa cgatgaccaa atcctttatc caggttaaat acaaggaaac
     7201 ccttgatttg ctgtaccgga aattgccgat gtttgaacgg atcggccccg cggcatttaa
     7261 aaaagactta tccaatatcg gcgccttatg cgaagcgctg ggtcatccgg aaaggcggat
     7321 caaaagcatc catattgcgg gcaccaatgg taaaggcagc gtttcccata tgctggcctc
     7381 gattctgcag gaggccgggt ataaaacagg attatacgta tccccgcact acaaggattt
     7441 cagggagcgg atccggatca atggaaaatg gatctccaaa cagcatatcg ttgattttgt
     7501 tgaaaagatc gggccggaca tcgaccggat caaaccttct ttttttgaga tcagtgtggc
     7561 catggctttc gattattttt tcaggaaccg ggtggatctg gcagtggttg aaaccggcct
     7621 gggtgggagg ctggacagta ccaatatcct cattccggaa ctcagcatca tcaccaatat
     7681 cagcctggac caccagaacc ttcttggaga taccgtggaa aaaatcgcct ttgaaaaggc
     7741 cggcatcatt aaaaaagggg tgccctgcct gatcggagaa taccagcgca agacccaacc
     7801 ggtatttaaa cgggttgctt cttcgttagg cgcaccggtc cggtatgccg accgccatgg
     7861 aaaagtcatt aaaaccgctg cgggccacta tcagtatctc agcaaaaagg ggccgcagga
     7921 gtccctggaa ctgctcacgc ccattctggg gccctaccag gagaaaaaca tcaataccac
     7981 gcttgaagcc atacgccttt taaatgaaac gggagattgg gctgtatccc atcgccacat
     8041 cctggaagga tttaagaatc tttcagaaaa cgcgcccctg accggccgat ggcagtggat
     8101 cggcgggcac ccgagggtcc tggctgacgg ggcgcacaac ctggccgggc tgcagtattt
     8161 gttcaaagag ctggtaaaaa cggaaaaggg gcaagtccat tgtgtctttg gtacggtgcg
     8221 ggacaaagcc ctcgatccgg tgctggccat cctgccccgg catttcacct attattttgt
     8281 aaatgcggac cttccaagag cgatgaactc ggacttgctg gctgaaaagg cagcaatgca
     8341 tgggcttcaa ggatcttctt acggtaaagt aagcctgggg cttaaggccg ccaaaagcgc
     8401 agcccgcggt aatgatcttg tgctggtcac gggcagcata tttgtggtgg gggaggtgtt
     8461 gtaaggaacg ataggaagca tagctgttgc cgtatccgaa gaaggattcc ggatttcctc
     8521 cggcaaccgt ttattaaaac cctgattaca ggaaacggga aggcaacaag caaataaaaa
     8581 ctgaaaaagg cgggtgaaga ggacacccaa tgcttacaac taccggtcca acaaaaactc
     8641 tgtgaaaatc ttttccttgt tccggtccac gatcaagcgg taagtcccgt tcggcaagcc
     8701 actcaaagct gcatgcactt cgttttgctg ctggattcct tttaccggca tggctttgcc
     8761 ggcctggtcc cggacttcaa aacgcaagac atcaaactgg tttttcagcc ggatgaccag
     8821 ttcatcctgg ttcaggtcct ggtagatctc aaaaggactg tccttctctt tgtggatcat
     8881 gacaaaactg ctgggcctcg aaaccatttt gtttttagcc acggtgacac ccgcgggcgc
     8941 cagggcgggg ctggattggc cggccaggaa attagaaaaa ccaagcgtaa gtaaggcggg
     9001 gagtagtatt tttttcatat atattaatta taattttgaa aagtcttgat tatgaaggga
     9061 tgatatctgt tgcaggatgt tgtaaatcaa agataataaa cattataatt aatcataata
     9121 tgtttaaaac tttttttttc cttctttcgc ttaccttgct gtaatgaaaa aaatcgtcat
     9181 cggcatatcc ggttcgagtg gcgcggtcta cgcggatttg ttgctgaaaa aactggtgtc
     9241 cctgaaagac caatggtcgg ttgccggggt ggtggcgagt aaaaacgcaa gaatcaattg
     9301 gcgactcgag ttgggaaccg aaatggagat cccttcttcc atgactttat atggacagga
     9361 ggattttaat gccccctttg cttcgggttc cgcccgttac gacaccatga tcatctgccc
     9421 ctgttccatg ggaaccttag gccggatcgc gcatggaatt tcagatgatc tgctgacccg
     9481 gtcagcggat gtcatgctaa aggaaaaaag aaaactgatc ctggtgcccc gcgaaacccc
     9541 gctcagcctg atccatctga ggaacatgac cctgctcgcc gaatgtggtg cccatatcct
     9601 cccggccatc ccttcttttt actccggtcc ggaatcgatt aaagacctgt tggccacggt
     9661 ggtggaccgg gccctggacc tggctggttt cgaactggat agcaaaaggt ggggggatta
     9721 atccaacacc tgtttaatat tccggttgac ggcaatgctg gcattcagtt cgaacccggc
     9781 tacgattaca aaacaattga gctggatata aagcaggata atgatcaggg cgccgatgga
     9841 cccgtaaaca aggtgaaagg acccaaaatt gttgatgagg gcggagaaga tgagggatat
     9901 gatgatcgag cttaccgtgg ccagcgtagt ccccggggaa aatacccgta attttttttt
     9961 gagggagggc ccgtaccgga aaatggtagc aaatatgcca tggaacatca ggatggtgat
    10021 caaccaccgg acgatctcaa agcccaccgt cgaatatttc tgcaggaaaa ggaaatggat
    10081 cacccagttg gtctgtttcc cggagaccgg gttctctatc acccattgtt tgctgctgaa
    10141 gtagtctacg agtccgttcc agatctggtt gccgaaaatg atcatgacca cggaaatgat
    10201 gaccagcagg gccaccagta cgaccagttt gagggcatcg agccgctttt gccagaaatt
    10261 ccttttttta aacgtttcgt cataagcttt ttcaaaaccg gccatcatgg tctgcatggc
    10321 gttggtggaa aaatacaggg ccaggataat ggacatcacc gacagcaggc cgttgcgggg
    10381 tatggaaatg acatcctcta tggttccgaa caggaagggt tccacctggt cgggaataaa
    10441 ggactgcaac tctttaaaca acatatcctt gatgccttct accgggagca gggggatcag
    10501 ggtaaagagg aagagcagga aggggaacag ggccagcaaa aacgaaaagg ccatggaatt
    10561 gactttgtgg atgatctttt ccctttgcga ttcatggtat aaaaacacga ggatatcata
    10621 gaccggaatg cccgcaaatc ccggaaggct tgatttttgg gcccagccca cgatgttttc
    10681 gagccgtttc ttaccggcta acttttcgag tttattgaat atgggcagtt tcgattgcat
    10741 ggctgatccg ttcttgaaga aatccggggc agggtaccct tttcccggtc aaagaatcca
    10801 gaaaacacaa cttgatcgtc gcggcacaaa ggagctcgtt tttttcatta aaaatttctg
    10861 aataaaaact gatgtcctga tgcggcagcg cgcgaaggct ggtcctgatc cggagttcct
    10921 ggtcataccg ggccggccgc agataacgca cctgcatgga catcacaggc atccagatcc
    10981 ggtgcttcgt ttccagttcg tggtacgtga gccccagggc gcggatccat tccaccctgc
    11041 ccacctcaaa atactcggca aagcggccat aatagacaaa acccatctgg tcggtctcgg
    11101 cataccggac cctaaggcag aactctgtgg agatttcgat tttaggcatg ggtcattctt
    11161 aaaatcgggc aagacataca atgtggtcct ccccgggcgc gcgcgagttc ggcgctgggt
    11221 atcgtgatga tcgttttctc cagccgggag acttcggcag aaggcagacg gagaaaatcg
    11281 ggtgcgtgca gaatccggta gcctgaatcc cggaaaccct tgatcgtgac cgcattgcgg
    11341 tcataggtga tagctacccc cggtttcacc gcgagcaggt tacagccatc ggtccattgt
    11401 tctctttcct gggagggact ttcacccccg cccacccagg tgaactcgat tttattgctg
    11461 atttcattcc ataaaaattc cttcagggtg gcataatatt gtttgtcccc gttgatgcgg
    11521 tgcacttcca cataagaact catcccatcc tgcacgatcg ggcggtaggc cacaaaccgg
    11581 tgatgatcca cctgggtgaa aatggtatcc aggtgcataa acgaccgttc ggccggaata
    11641 ttgatctgga ctacattgct caccgcgcga tgtgaaaata accacgattt gagggaatgg
    11701 aaggcatata aattggtgcg ttcgctgatt ccgatcagta catattccgg attcaggatc
    11761 atcacatcgc cgccttccat ggatacggct tcccctttgc gggacggggg aaaatcctcc
    11821 aggatgttca gatcgatgat cttgccgctt tgcctggggc tggaaaacag ggggtgctga
    11881 tacaggataa accgggtcag gtagttttcc ctggagcggg cgaccttggc tgccttggtg
    11941 atcaccatat attcgcccag cgtcaccgcg atgtcccggg tgaaaagaaa attcgggata
    12001 gggtcaaaga ggatgcggtc atccatggca tcgtagccgg tgatcaggac ttcggcgagc
    12061 tgatcatccg ggagctggcc cagtttcggg gcaaaggagg gcggcaattc ttcgtattcg
    12121 atcaggcttt ttagcatttt ttcccttgcg gtcaaatcca ccccgagggc ttcacggagc
    12181 aggtcctggg tttcgaggac cccttcaggg ccaaccagtt ttcgaaggac atcgacgaag
    12241 gttttgtgtt cggcctgcat atccggaaga aaaacgatat cgtcgaacag gagttcagca
    12301 gattgtttgg gtgaaacccg gaggatgccc tcatcgggcc ggtgaacgat cactcgtttt
    12361 aaggacgcga tttccgagtc cgcgaagata tcaacccctc ttttcatgcc tgattcattg
    12421 gttaatgcaa aattcgatta tttttctcta ttggtgaccg gctccggccg gcatctgacg
    12481 ggggatcagg gctcatccgc agccgggcaa cgcgttaatt tgtatttttg ttcgctcaaa
    12541 gaaccagttt ataagggatc atgcttaaaa aaatcaaatc catcttcatt gttgaagagg
    12601 aaacatcgcc ggttcgcccc ccggcttctt cggttcaggc cgaccaggag gacaaaagga
    12661 tgaaggaggc agtggatatc aggatatcgt ccgggaaagc cggttcaccc ccggaatccg
    12721 gttccgaaaa caggtttctg cacgtgctgg ccgaagccat tgaaaaaagc aaccagcaag
    12781 gctttgatta cctggaattc agacaggccc tggtcaatct gtccaagctg agcatggacg
    12841 aacctatccg gtacaagagc gcctacgcgg cggcgcaggc catgggggtg tctccttccc
    12901 agttgatcag cagtgcaaaa ggttacctgg ccatcctcga aggcgagtcc aagaagtttg
    12961 ccattgcgga acaaaaccag cgtacccgtg tggtggagga aaggaaaaat gaactggaac
    13021 aaatttctgc ggaaatccga agcaagcagg atgccatcac caaactgcaa gccgaagtgg
    13081 agaaactaac caaaaaactg gaagaaaaga aaaacgaggc ccgggtcatg tcaggcaaac
    13141 tggaacaaac caaggtcgag tttgaaacgg cttataaatc cctgtttggc cagatttcag
    13201 aagacattca aaaaatggat caatacttaa aataacccta tttttatatt ttcctatgga
    13261 tagtaccgaa tttaagccca aatcattctg gaagaggcct gaaggagtga ccggagccat
    13321 tgtgttgggc gggattgtga tcggcggggg gatcctgctc gcctccgcac tgccgttgct
    13381 agtcagcctg gtccagtcca ccctcggcct ggtggtcacc ctgctggtat tgggcgccat
    13441 catatacatg gtcctggacc ccaagatgcg caacctggtc tggtacatgt acaaaagcac
    13501 catgcgctgg atcacgggcc tttttgtgaa aatcgatccg atcgggattc ttaaaaacta
    13561 cgtcgaggac cttaaagcca acctgactaa aatgaatcag caaatcaacc gcctgcgcgg
    13621 ccagatgcat aagctgaagg aactgatgct gatcaaccag agagaaatac aaagcaacct
    13681 ggagatcgcc tccaaggcca aacaaagcaa tcagcaaaac gtcgtgattc tcaaatcccg
    13741 caaagcaggg aggctgaggg aatccaatat gaaatacgag gatctctata aaaaaatgga
    13801 gatcatgtac cgcgtgctca ccaagatgtt tgaaaactcc cagatcatgc aggaggacat
    13861 tgcagaccag gtagaggtga aggagcagga gaggaaggcc atccatgctt ccaattcggc
    13921 catgcggtcg gccatgagca tcatcaaggg ggataaagac aagagggcca tgtttgacca
    13981 ggccctggag gcggtggcgg acgacgtcag ccaaaaagtg ggagaaatgg aaaggtttat
    14041 ggagctctcg gacaatttca tgaaaagcat tgaccttcaa aacggggttt ttgaggaaga
    14101 aggactgaaa atgcttgaaa aatgggaatc ggaaggcgta tccaaactgc tgggcagtga
    14161 aaaagccagt atcctttccc gggccaataa tgaagcggat gtgctggacc tggaagcccc
    14221 cctgacccgg cccgaaaaag ttggagccca taaaaaccag tacgacactt tttttgagga
    14281 ttgagccgga atttattggg tcttcgattt gttgtaattt cgaattctaa acaaatcctc
    14341 attaaatcat ggcaacccgt cttactggat tttcacgatt tttaattacc ctcctcatcc
    14401 ttggattgat cgcttttgcc ggttattacc tgcttaataa aaccggggtc ggagccaaac
    14461 tgaaagatca ggcagaacaa gcagaatccg gccagtccac gactaccgga gggacaagtt
    14521 ccgctccatc cggagccttt aaagacgccg tcaaagtcgg cgtggtgacg tgggggggat
    14581 atgccggggg gcaatatttt aatgaaggat tcaaggccaa ttcccggtcc aggtttgtac
    14641 aggattatgg ctttccggtt gaatttaaga tcctcgatga tgtgcccgta tcccgggagg
    14701 catggaaaaa cgacgaagtc aacctcctgt ggtgcaccgt ggatgccctg cccacagaaa
    14761 tcgccgggct ttctgatttt gatccggtgg tggttttcca ggcggactgg tccaggggcg
    14821 gggacgccct ggttgcccgc cgcggaatca actcggtgaa tgatctgagg gggaaaaaga
    14881 tagcggtagc ggagatgacc ccttcccact ccttcctgat ctggatgctt gaggcggcca
    14941 atttaaaagc aacggatgtc cagattgtca aacaggcctc cgccattgac gcggcccagg
    15001 tctttaaaag ccaacaggtg gatgcggccg tggtctggag ccccgacgac caggcctgcc
    15061 tgcaggccgt agccggatcc aggatcctgc aaagcaccaa gtccgcttcc aatatcattg
    15121 cagatgcctt tatcgccaaa aggtcctggg tggaacaaaa cagggataaa atgcaaaaac
    15181 tctacgaagg ttggatgaaa ggggccgccg aaatcaatgc gaacgatgat aataaaagga
    15241 aggcggccaa gatcctggct gaaaacttca gcggatttac ggaagacgac gcctatgcag
    15301 ccatcaataa cgtccgcctg gccacgcacg gggacaacca gaattttttt ggactgaacc
    15361 cggattataa aggggttaca ggggaacaat tgtatacccg gatgaccaat tcctacaaag
    15421 ccctgggtta tgccgaaggc cggattccga actggaggct gatcagtgat cccttatcca
    15481 tccgaaatac cagcctgacc ggtccggatc atgccgccga aggtcagaag gcttttacca
    15541 aggttacgga agaggccgga aaagaaaagg aagccattgc gaccaaaatg gtgtccatca
    15601 ccttccggag cggggaatcg gccctggatg aaaacgccaa gtttattgtg gacaaggaaa
    15661 tggttgaaat tgccaaagcc tttgccaaca gcaggatccg cgtggaaggc aataccgaca
    15721 atgtaggctc tgtcgcttta aatcaagccc tctccaggag aagggcacag gccgtggtgg
    15781 attacctggt agccgcacac ggaatgaacc gtaaccgttt tgtagtagtc ggtaacgggc
    15841 ccgataagcc ggtcgcttca aacgatacgg aagagggcaa ggccaaaaac cgccgcactg
    15901 attttcaatt aatcggcgaa taagaatcca tggacaactg gtttaagctc cggggtacga
    15961 atgcagggac ctccggactg ctgatctcag cagtgggttt tatcattacc atcgccctat
    16021 ggtgggtttt ggctgaaata ttttccaaac aaaaacccgt cctggagggg caggatgaag
    16081 taaccctctt cgataccctg ggcggggatc agaaggtaaa actctggaac gagccgggtg
    16141 ctgagcaagc cgcaggcacc cgttatgaaa aggtttatcc ccttcttccc aggccggaca
    16201 aaaccctttt gtctttcaaa cccctgattg cccaggacaa attggtatcc aatacccttc
    16261 attccttctg gctcaacctc caggggtatt tttgggcgat tttgctggct attcccattg
    16321 gcatggtttt gtccctattt ccgcttttca gggacctgtt ttcgaagcag gtcgatgcct
    16381 tgcgttttct gccgctctcc gcgttgaccg ggcttttcat catctggttt gggctggggg
    16441 atccgatgaa gatcgccttc ctggccgtgg gcatcctggt ttatctcatt ccggtagtga
    16501 tgcagcgcac cagtgaagtg gatgaaacca tggtccagac ttcatttacc atgggggcca
    16561 gcccctggca gcagattaaa aaagtatttt tcccggcagc gctcagcagc ctgattgacg
    16621 atatccgggt gcttaccgcc atttcatgga cctatatcat tattgccgaa ctgctcaacc
    16681 gggaatcggg catcggagcc ttgatttata ccaaagcccg gttgggccag acggaccgtg
    16741 tcttcgccgt cctcatcgtg attatcctga tcggcttgtt tcaggaccag ttgttcaaag
    16801 ccatagaccg gatcatcaat ccattcaaat attataaaaa caaggtggat gggctgcaag
    16861 acgggaagat tggcgtattc gcctggatcg tggcgatcat cgcagggatc ctgggcggat
    16921 tttctccgtc actgggcatc ttatcttatg tggcctgggt cctggccgcc gcaggcaccc
    16981 tgtttatcct ttggggtttc attaagatta ataaagcagt ccattgattt atgattgagc
    17041 ccaaaactcc ttccttgccc gacctgatcg aactcaggaa catcagccag tcttatgacg
    17101 gcggcaagac ctatatcatc aaggatctga accttctggt tgaagataag ccggcccagg
    17161 gccagtttgt gatcattctg ggcatgtcgg gatgcggcaa atccacgctg ctacgatata
    17221 ttgccgggct tcaaagtccc acggaagggg aggtttacct caggggaaaa ttaaggcagg
    17281 attccgatca tgtcgggatg gttttccagg cttattcttc ctttccctgg atgacggtaa
    17341 aggagaatgt ggccatgggc ctggatttcc agggtatgga ggagaaggaa cgcaacctga
    17401 aagcgctgga gatgattgaa ctggtgggcc tggccggaca cgccgacaaa tttgcccagt
    17461 acccggttct ttccggcgga caattgcagc gggtggccat cgcccggagc ctcctggcca
    17521 accccgacat cttgctgatg gacgaacctt ttggcgccct ggacatcaaa acccgcattc
    17581 aaatgcagga ccttttgctg accctttgga acaagttcca ttccaccatc atttttgtca
    17641 cccacgatat ccgcgaggcg gtgtacctcg gtgacgacat atactttatg aaatacgcgc
    17701 cgtccagcat tgtggaaaag atccatgtag accttccgct ccaacgagac cacgaaacca
    17761 ggcgactgcc ccggtatacc gaattggtct acgaagtgga agataccatg ctgcgtatcg
    17821 cagcaggatg acctgcaggc acttcttcat ccggttcatc tgtctttttt atctattttt
    17881 ggaaaaatgt atttattcca atgaaactga aattattgtt tgtttcccta atgacctttg
    17941 ccggctttat ggccgccgcc caatccctgg accgcccaaa actggtcgtc gggatcgtcg
    18001 tggaccagat gcggcacgat tacctctacc gttactggga tcaatatggg gaaggcggat
    18061 tcaaaaggat actaaatgaa gggttttcct gcgaaaatgc acattataat tatgtgccta
    18121 cggtcaccgg cccgggacat gcctcgattt ataccgggac cacccctgcc ttccatgggc
    18181 tgatcggcaa tgaatggcat ctccggaaca gcaggcgcgg gatgtattgt gcccaggacg
    18241 acagcgtaag cgcggtcgga ggaagcgaag ccgcgggcaa aatgtctccc aaaaacctat
    18301 tggccaatac gatcaccgat gtattaaaac tttataccta caaccgggca aaagtcatcg
    18361 gggtttccgt caaggaccgt ggcgccatca tgccggcagg ccatctcgcc gatgctgctt
    18421 attggtacga cggcaatacc ggaaaattca tgaccagcac ctattatatg aacgcattgc
    18481 ctgcctgggt gaatgctttt aatgaccgga acctgggcaa acaacttttc agccagggct
    18541 ggaatacgct gaaacccatc gggacttatg tcgacagtga gcgggatgac aatccctatg
    18601 aaggaaagct gaacggggaa gccaatgcgg tattccccag gaatatccct tttaaggacc
    18661 ccacttcgta ttatacttta ttgtatactc cagccgggaa ttccctcgtc acggaatttt
    18721 caaaagcggc tcttgaagcg gaacagcttg gccaggacga ggtcacggat tttctcgcga
    18781 tgagttattc cagtacggac tatgcggggc atctctttgg ccttcattcg gtagagatcg
    18841 aagacatgta tctccgcctg gacctggaga tggaaaagct gcttcggttt ctggatgaga
    18901 aagtaggcca cgataaatat gtggtttttt tgagcgcaga tcacgccgtg gcccagaatc
    18961 ctcaatacct caagcataaa aatttaccgg tggattattt ccggggctgg aggatgatcg
    19021 attccctcaa aaaattcctg gaaaaaaaat acgatccttt cctcatcgat gggtacagca
    19081 atggccagat ttacctcaat gaagaccgga tcgtctctcg taaactggac cggaaggcca
    19141 ttgcggccga tctcaaaaaa tacgtaaggg cctgggacgg agtgattgat gtggtggacc
    19201 gggatgatat tccgttctta acgggtggta atccggtatt cccgatgatc caaaaagggt
    19261 tttatccccc cctgtgcggg gatttttatg tgatcatccg gccaggctgg ttcgaaagca
    19321 gttccatgac cggcaccacc catggcagcc catttaccta tgatacccat gtgccggttc
    19381 tttggtatgg ctggaagatc cctgcgggca agtcttatgc aaaatatgaa atcacggata
    19441 ttgcctccac cctcagcttc ctgttaaaaa tccccttgcc cgacgcggca accgggaggg
    19501 tgatcacgga attgtttgac tgagcgtcct gtccgggatt tggaggccct gtagccgcag
    19561 tatcaatatt gggccttcag gaattttacc gtgtttcttc tcttttcttc gtcttggacc
    19621 tggagaaaat agaaacccgg agccaggccg gaaacattta tttgattgtg gacttctttg
    19681 gttaccaagg attgtaagct gcgaccgttg acatctctca ggctgatctc agctgttgat
    19741 tggagaccaa aaatttcaac attcaagatc tgggaaacgg gattgggaaa cagggtggtg
    19801 gacagcccgg tggtggaaac aatccctgaa gtcagggaac ccgcagaggg cttgacgagg
    19861 agggcatagc aggccgcatc gctgctggcg gtgttccatc cgtaaacccg tacataataa
    19921 gtcccggtaa tcaggttggt attgatggtt tcattcgcaa gaccgccggt ttgggagagg
    19981 cctacggtgg tcccagaact gttgaggatc ctcagatcgt aatctgcagg caattggctg
    20041 agaatagccg tataggcgcc tgtcgcggga accgtaaact tgtaatagtc attttcccgg
    20101 gcggggaaaa tttttccata gatcatggca tttaaggtga tcgacggagc ggtggtgcga
    20161 ttgtcattgc tggcattgtc ctcgagggcc gggcaatccg gggtcgcgga ggaagccgtg
    20221 gtaaaactag attggatgaa ctcactgtta cccgaactgc aattggtttt tactctccac
    20281 aggtaggctg tggaaggcga caaaccggaa atggacgtgg atgttccagt caggctggcc
    20341 cccgcagtcc agttgaggtc ggaagaggtt ttatattcca cttggtaccc ggatgcaccg
    20401 cttaccgaag cccaattcag ggtggcggag ttggagctga tgtttgtagt attcaggccg
    20461 gagggcgtat tgcatacagg aagggccagg gtactcagag tcgattgggc atagccgccg
    20521 gaaccaaaac tgcaattggc ccttactctc caatcatagt tggacgaagc atttaagcca
    20581 gtcaggttat aggtggtggc gttcaaggct gcggaaagac ttatccaact gggggaggcg
    20641 gaggccttat actcgaggga gtaatgactt gcacccgaga ccggagacca ggacaaagtc
    20701 gctccattgg ctgtaacgac tgaagtggat aaccctgatg gggtaccaca taccagcgga
    20761 gcctgggtag ttacagaaaa ttggctgtac ccgctctgca ggccgcccga acaattggtt
    20821 tttaccctcc aatcgtaggt ggtcgatggg ctaaggccat taaaagtaat cgaattcgta
    20881 gatatggcgc cttgtattac actccagttt gatgctgccg cttgtttaaa ttccagggta
    20941 taatttactg caccgggcac ggcattccaa tggaaagtgg caccgttctg agtgatgttg
    21001 gaattggtca gcccggaagg tgcattacag gccggtgccg ctatcgttgt gaatgcagcc
    21061 tgcgtaaagg cactgcttgc tccggaacaa tttgttttca cccgccagtc ataagcggtg
    21121 gaagcgctca atccactgat gtcataactt gccgaagtaa tattgaccgg catggtgatc
    21181 caggaagcgg aggtttttgc cttgtactcc aggctatagc cggttgcgcc ggcaacggca
    21241 ttccaactga gggttgcccc gcttgatgta atggaggtgc tggctaagcc tgtgggtgga
    21301 ttacaagtgg cttgcggtcc gcccgcggtg gtgaaggtgg actccgtaaa tccgctgatc
    21361 cccgaagtgc aattggtctt tactctccaa tgataggaag tggactctga aagaccggtg
    21421 aggttgaggc tcagggaaga agtggttccg gccaacaacc aggtggggga ggtcgtcttt
    21481 ttatattcaa tggtataatt aaaagcgcgg ttggctgcag accaggaaag ggatgccccg
    21541 ctcttcgtga tcgcagaagc ctcgaggccg gccggcggca aacaactcac cgcctcagcg
    21601 ataaaactac cctggctgta aggaccctgg ccatttgaac aattggctct gaccctccag
    21661 tcataaccca ctccgttggt caggttgatc aaggtcctga aaagggagga ggtagcggtg
    21721 attgacgtgc tccaaattga agcggtagat cttttatatt gaacttcata agacgaagca
    21781 ccggcaacgg cttgccacct cacggtcgtg cttgtactgg tattgttgct gctggtcagg
    21841 ttggaagggg cgatgtccat gcaggttaag gtcagggtat taaaattgcc ggcggcaaaa
    21901 tcggagttac cgcctgaaca attggcccga acctgccagg tatagctggt tccttgctga
    21961 agtcctgaaa ggttgaaggc agtcgagctg gttgcattgg tcgccgtaat ccagatggtg
    22021 gaggaatttg cacggtattg aacggtgtag gagcttgcac tttcggatcc ggtccagctt
    22081 aaattcacgc tggaagaagt aaggttggaa atattgaggt tttgaggggt ggcacaaacc
    22141 acgatgggtt cgcaagggcc aaggcaggtg gcggcatcca ccctgctccg gatcaggttg
    22201 cccggctgcg taccgaaacc attattgagg ttggtacctg aagtggtcag gtggcaatat
    22261 gacatgatcg tgcctccgtt gaccggcgca ggtccggcag cacatgtgcc ctccggcgta
    22321 ctgcaattgt ccagagcccc tccctgccag ctgcaactgt gcgtatgggg agaacccagg
    22381 ttgtgcccaa gttcatgggt gaccaccagg gaggtccagg aataggtagg gaggttattg
    22441 taggtggatt ccatctccgc ataactaaat ctgccggact gggagatctg attgtaactg
    22501 tcgcacaaaa catccagcca ggcaatcccg gcaagtccgc cgctgggttt gatccggaca
    22561 aattgcgcga tgtctccatt aaagttatcc cgccggtagc ttccgaagga gtacaaagct
    22621 gaaagagagc tgctgctgct gaagggttcg ctggtggtcc agataaaaac ctgtgaaagg
    22681 atcaccatca tgttttctct tgtgtagagg gtggaaacca cattgaataa accggtcaaa
    22741 aaattggtcg cgccctggat tcctccatgg gcatcataaa cgctttttcc agcttccagg
    22801 taaatgttga cacaaccggc tgcatttctt tgttcgaggc cgggcttatg cagttccttg
    22861 agttttgcct ggtaattggg caaagcttca gaatggcagg atatcgacgg agccgttgtt
    22921 ttactggcgt cgtacactac aaaactcccc cggcggaaag ctttcgattt acccaccgtg
    22981 tactgattgc cacccgttga atttatgacg gcaaccactt catcttcaaa aaagctgatc
    23041 gcgaccaatg aacccggctg cccttcaata tgcccgttat aatggagccc gggagtgaaa
    23101 ggaaggcttt tatcctgatc ggtactgagt ataaaaccat ccgaaaacag atgtgttgga
    23161 ctgagggata aaggcaggta tccatccggg gtgggcaaat taagtttaaa agaagcaggc
    23221 gccgacgatt tgagttgttg ataactatcc tggttatact caagtaccct gaccctggta
    23281 ttctgcctca aggcgttcca acgggtttcc tgaaccttat cgattttaaa taaatccaca
    23341 gggagctccc ttccggataa agggaatttc gccagaaata acacacccaa aaggatggat
    23401 atggtgcagt ttttggtttg gctcattatt caaccggttt gaagcgaatg gagcaacaac
    23461 tgggaaggcg aagataaaaa gattgaaaca ggccggggta taatgcttct ttttttattt
    23521 gattttaagg tatgttaaat atggaatatg ttcctgattc agaacccggt aggatgcttt
    23581 tgatgccatt ccgttttatc ctggtaatat ttagcaacag ccaatacttc cgattcgtga
    23641 aataacttac cggtaaagct gatactggta ggccttccgt tccggaaccc attggggacg
    23701 accacgcagg gatgcccggt ataattggtc acggaaaggt tgcgaccggc ccatgaaggc
    23761 gcgatataaa catccacttg gttaaaaaat aggtccatgt cttcaatcag gagggtccgg
    23821 aaccggttgg cctggatgta ttcgaccgcc ggaataaaac gggaggtccg gaatacattc
    23881 ggccaggcgt tccggatctg ttttaccatg agatcgtccc tgccgctgcg ggtaagttca
    23941 tcgaaggtcg caccggcctc cgcggtaagg atgatgccca ggtccggggc attgggtaat
    24001 tccaggggta tcagttcaat gcccgcattg gtaaaaacgg tcaggctgag gctgtcctgg
    24061 acatggaagg gataaggctt ttcaaaatcc tgctttaaat atccgattcg cagtttgcgg
    24121 atgtctttat tgtatgcgta agggaagggg acaggactcg ctgatggccc ggaaaggaag
    24181 gtgcctttga tatgctggta gacaatagcg gcgtcttcta cgctccgcgt gatggggcct
    24241 atcttatcca tggaaggaca caggatcata caaccttctc cgggtacggc gccaaagctt
    24301 ggcctcaggc ccgtagtgcc gcatacggtg gaaggcgata cgatagaacc cagggtctcg
    24361 gtcccaatgg caaacggcag gcaaccggca ccgacggcag aagcagaacc cgccgatgag
    24421 ccgctgcttc cccctgtggt atcccaggga ttccgggtca ttcccccgaa ccacacatct
    24481 ccccaggcga gttcacccac gctcattttg gcgatcagga tgcttccggc ctcttcgagt
    24541 ttggtgatca ctgccgcatc atagtcaaaa acctggtctt taaaggggac ggatccccag
    24601 gtggtaggat atcctttttt agccaggagg tcttttgccc cataaggaat gccatggagc
    24661 agtccccggt attttccagc ccttaactca gcgtccattt ttttcgcttg cgcgagtgca
    24721 tattcttcag taaaatggat cacgcatttt aaggcaggat tgtaagtcct gagcctttgg
    24781 ataaaataac gggtaagggt ttccgagctc acctttcggg atttgaccag ggatgccagt
    24841 tgatgcaggg tcatgaatgc ataatcctcc ctcgcggccg ggttagccgg taagggcgtg
    24901 aacacggctt tggtggtatt ggcaaccggt ttataccggg caggataagg catgaaacga
    24961 agtgcgggga gcacccggtt ttccagcgca gtcttccgca aggattcaaa agattccagg
    25021 ttttccccga gatccggcaa caggctgtcg atttctgctg cgctgaattc aagtccgatc
    25081 agtttttgcg cgctcagaac caagtctcta tgaattccgc cggcttgttg gtgcaaagta
    25141 aagccgacaa ccagcaggat aaaagcaagg gagaaaagat ttaagggttt taatttcatg
    25201 attcgtgact tattctttca aggtaaaaaa atatctggtg ttctgtttct atttttggct
    25261 ctcttaaatt catgctatgt tgaactgcca gaaagaaaag ttttttattg cacaggatgt
    25321 gacgtatctc aatggggcct atatgtcgcc ccagttgagg caggtggagg aagtgggcat
    25381 tcaggccgtt caaaggaaaa acaaaccctg gacgattacc cctgctgatt tttttaagga
    25441 agtggaagcc ctgaaactgt tatttgcacg cctggttcat accgatcagc cggagcggat
    25501 agcggtcatt ccttccgtat cctatggaat tgccaacgcc gcccgcaata tttcttttga
    25561 aaaaggcgac catattttga tcctcgagaa acaattcccc agcaattatt atgagtgggt
    25621 tgacctggcc cggaaaaaag aactcgtcct ggacgtagtc aaggccccgg attctctggt
    25681 tgaccgcgga caagcctgga atcagaaagt aatggaaaac atcaagcccc gaacacgggt
    25741 ggtcgccatg gagaacgttc attggaccga tggcacctgg tttgatgtgg cccaaatcag
    25801 caaaaaaata aaagcccagg gtggtttttt tatagtcgat gcgacccaga gcctgggtat
    25861 tttcgatttt gaccaaaatc tggtgcaggc cgatgccgtg attgccacag gatataaatg
    25921 gttgctgggc ccttatgcca ttggttgtgc ctattatggc ccggcttttg accaggggga
    25981 gcccctggaa gaaaattgga tcaataaaca atacagccag gaatttgaaa ggatactgga
    26041 ctatgaagaa tcttaccgac ccggagcagc ccgttatggt atgggcgaac aatcacagtt
    26101 tgtgcaggtg ccgatgttgt gtgcatccct gtcccagatc ctggaatggg gtgtggctga
    26161 aatgcaagcc tattgcaggg gcctggtgga ccaggtccta ccattactgg atcaaaagga
    26221 cctctggatt gaaaagaagg actggcgcgc cagtcacctg tttgggctca ggactgccgg
    26281 aggtttcagc ccggctgtaa aacaaaaact attggatcaa aaaatttttg tgtcctaccg
    26341 ggatgatgcc atccgggttt cccccaatgt gtataatacc aaggaggatt tgataaaatt
    26401 aattgaaacc ctccgttccg ctcactaacc accgaccact gactactgac tactgattat
    26461 tgactactga ctactgacta ttgaccactg accatccact tcttatcttt aactatgcct
    26521 gccaaaatat tcttattgtc cctgctggcc ttcacttcct gtgcaccaaa acaaatggaa
    26581 gtggacggcc tgatcaccca cgccctgatt tacgatggaa cgggttcacc accagtagcc
    26641 ggatccctgg cctggaatgc ggataccatc gtaggaatgg gcgaggtggg catgatccgc
    26701 gcgaagaaga ttattgatgc aggcggaatg gcgcttgcgc cgggttttgt aaatatgctg
    26761 agctgggcgc ccgaaaccct gatccatgac ggcaaatcgc tcagtgattt gaagcaggga
    26821 gtgaccctgg aggtttttgg ggaagggacg agcatggggc ccatcaatga agccatgcgg
    26881 caggatatgc tcgcttccat gggggatcac ccttatgaga tcggctggac caccctgggt
    26941 ggttacctgg actttctaga gcaaagaggg gtgtcctgca acttcgcctc tttcatcggg
    27001 gcggctacgc caaggatcca tgaactcggc tatgccaatc gccaggctac tccagacgaa
    27061 atgaacagga tgaaacaact ggtagcccag gccatggaag aaggggccat gggtgtggct
    27121 tcagccctga tctacgcgcc gggtacctat gccaatactc aggaactcat cgaactggcc
    27181 cggactgcgg ccgggttcgg gggtatgtac atctcgcaca tgcgcagcga aggcaatcat
    27241 atctggtccg cgctgcatga attgtttacc atcagccggg aagccggcat ccgggctgaa
    27301 atttatcatt tgaagctgag cgggagaaac aactgggaca aacaggatct cctggaacaa
    27361 aaaatagaca gcgcgaacca agccggtctg aaaatcacgg ccgacatgta taattatacg
    27421 gcaggcgcta cagggcttga cgcatccatg cccacctggt gccaggaagg tgggtacaag
    27481 gcctgggccg gccgactgaa agatcccgct ttgagaaaaa ggatcctcca cgaaatggaa
    27541 acggataccc ttggctcctg ggagaatttt tataaaatgt gcggtcctga aaatatgatc
    27601 accgtggact ttatgaacaa agacctcagg aagtatgtcg ggaagaccat tgcggaaatt
    27661 tcaaaagaca ggggcagttc accggctgaa gccattgccg atctggtggt ggaagacggc
    27721 agcagggtag gggtggtcta tttcatgatg tcggaagaga acgtacgcag aaacatccga
    27781 cggccttatg tgagtttttg cagtgatgcc ggcagcattg cggctgaggg aatgttcctg
    27841 gagaataatg tacatccgcg cacctacggg tccttcgccc gcctccttgg aaaatacgtg
    27901 cgtgatgaaa aactgatccc gctggaagaa gccattcgca aactcacttc cctgccttgt
    27961 gaaaacctca aagtcaataa aagaggtatt ctcaaacctg gatattttgc agacctggtg
    28021 gtttttgatc cggagacgat tcaggatcac gccacttatg aaaaaccgca tcaatatgct
    28081 accggtgtcc atcaggtgat cgtaaatggc attcaagtta ttaaagatgg ggagcatacg
    28141 ggggctaaac cggggagggc ggtgaggggg cccgggtgga aagggaagta aacagttggc
    28201 aagttagtaa gttagtaagt tagcaagttg gcaagttggc aagttgtcca ccctcaacct
    28261 ctagacccat aactttgttg ctgtgaccaa atcagcacat ccacagcaag aagacccgcc
    28321 catcctggtt tttgacggga cctgtatcct ttgtcattcc ttttttcaat gggtgatcaa
    28381 gaaggaccgt aataagattt tccggtttgc aacgctgcaa tcggaagcag ggaaggtcgt
    28441 tttaggtaat ctgaatatac ctgggggata tgatacggtt ttgctaaaac tgggagggcg
    28501 gatctatact tattcaacgg ccgcactgaa aacgctgatt atactaggtg gcttttatgg
    28561 atggctggga agggccggtt ttgtttttcc aaggattttc cgggattggg tgtacaggat
    28621 tattgcacgg aaccgttata ggtggtttgg gaataaggag tgtttggttc cggatgagga
    28681 gttgagggat agatttgaat aacgaaataa tgatggcctt gcaggccgta tttaagtgcc
    28741 ggtgcatgat attccagatc agggaggacc ctacccttca aatggcatga cagatgagaa
    28801 ggatgccgaa gattctgttt ttgagcaatt gaattcgccc tgaaggggca ccaccaccca
    28861 gcatcgggca tttcgcccga tgctgggtga tgagggtatg tatttcgccc tgaaagggca
    28921 tcattttaaa tcgtttttat tcatccctca ataacctaaa taaaatgcat agcaaaaggc
    28981 tattcattct ggaatccgga aaatgaatat cacctactcc tggaaacatc cgatcggcgc
    29041 tccatccggc atccgtgaag gttcgggggc ttcgaaggag gaaggatctt cccggtatcg
    29101 cctgatcatt tccatgatat aaccggcctg ggaggactgg ctggaggaca actgggattc
    29161 cgtgagtttg ataaaacgca aacaaggact cattacggaa gaagccaggt ttttatcggc
    29221 catcacggat aataatttat tgataaagat cctttcgtta ttcagtttga tttcctgttc
    29281 caggccattg gtgaccgggt gactgctgat cccgcggtgg atattctgaa ggtaggaaag
    29341 gaggttccag tgactggcat tgcgggcatt ttgttccacg atgcgggcta ggcgttccgg
    29401 ttgaaccagg aaacccaggg caaatccgga gatggattcc gccgccgcca gaggatcaaa
    29461 agggaggccc gtataggttt taaaattttc ccggtcccgg tcataacccg gcggctgggg
    29521 cgggatgagg ttcaggatat tttcaggcag ggacagttcg gatggactga tgcttttcag
    29581 caccgcattg agcgccaggg cctgctggga agagggtacc accctcgtga tcagctggct
    29641 gtctcccttc accgcgtagt tgtattccat tccgccaatc catttgctga cggcttcgac
    29701 ctgataacgg tggctgaggt acaaggggac caaaacgttt tcaagggtag ccatgggttc
    29761 acctgtacga agggtattca ggccaaattg ttgcagggcc tgcctgcgga cctggaggat
    29821 gcgctgcaat tcatccaccg cgctctgtcc gttgtcccag agatgggaat agggatgagc
    29881 gcttcccgcc ggccttgaat cctggtcggt caggtatttc aaattcaagc ggatggtctc
    29941 gttcatgatt tgatccaggg ctttcagctc atcggtcccc tgggggaaat cggaatatcc
    30001 ccaaaggatg gcccgtttat cccatgctcc gatccccaca tcataggcat tggaaaaatc
    30061 aggcttgcca tccttgaaat ccaccaccgg gtgaggatag tccattaccg acgccctgcc
    30121 gttaaaagtg gaagcataat tgtgggataa gcccagggta tggcctactt catgggccga
    30181 taactgacgc aatcgcgcca gggccatcgc ggtcaattcc ccggtattct catctccgtg
    30241 cccaaaaggg gaggccaggc cgcgggcgat cagataatcc tgtctgacgc gcagggagcc
    30301 caggctgaca tggcccttga tgatttcgcc ggtgcgggga tcggtcacgg tattgccata
    30361 agaccaaccc cgggtgctgc ggtggaccca ttggatcaca ttataccgga cgtccatggg
    30421 atcggcgtcg gcgggcattt ccctgacctg gaaggcattg atgaaaccgg cggtctcaaa
    30481 ggcttcgttc caccaacttg ccccttcgat aagggcggat ttgatgggct cgggacagcc
    30541 ggcatccaca taataaatga tgggtttgac gggttcactg aaaggcgcga agggatcctt
    30601 tttttcaagc cggtgccgca ccgtgagcat tttttttagg ggctccgaga tcggcgtcgc
    30661 ataattataa aaggaggttt caaaaaaacc ggaccgcgga tcaaaggtcc ggggttgata
    30721 tccatcatcc ggcaattcaa caaaggaatg atgctgatgc acgctcaccg cctcgggtga
    30781 gggggtcacg ctgcggatat actgacccgt ggctttaccg gtgaacgtaa gtaatacatc
    30841 ccattccgta ttctttggaa agttgcgggt cctgtccagg ttaagagcac ttctgcccag
    30901 gtccagccgg taagtaccct gcctggctga ttccaatctt ccggagacat catgcgcatc
    30961 ctgcatcaga aaaggggtaa ggtctatgta gaggattccg ttttcctccc gttcaatcgg
    31021 gaaaccccaa agtacggatt gggcaaaggc ttcttccacc gacttacgct ctgcctcatt
    31081 gtttgaaatg gcccggtagc tgtaattggg ttgtaccaaa aggagtttgt tggcggcacg
    31141 tttgaatttg acgattcgtt ccctcccgat ctgtccccgg tccagtccga ggtcattgga
    31201 ccctacgcct gaggataagc cctgcacgta caggaattcc tctcctactt ggcttacgga
    31261 gagggtgacc cgtccatctt tttccgaata ggtgaagggg aagtagccgg gaaagtccct
    31321 gatttcctgg gctggaaggg aggtggaacc aataagagcg atccataagg agaagaatag
    31381 gtttttcatc agataaaact aagaaatagt ggtcagtgtc cacgtcccga ggctgaggct
    31441 aaggtagcgg gaggtattca agggcagggg agttgatggt tctggggcta aagcccaatt
    31501 tgatctggac ttcaaacccc aagcctgaag gctggggtta gtcagcgcaa agtgattgcc
    31561 acctggcttc ccgctgggct aggcaaggtg actaccactg cttcaacccc aggcttcagc
    31621 ctggggtttg agctcaaggg agataattcg ggctttagcc ctggttgatc tgcctgcgct
    31681 acgaattact cctaaaactc agtgacctcc taccctccgt gcgctaattc agcgctaagt
    31741 gattccacct ggcttcccgc agggctgggc gagctggctg ctacacatcg acccaaagtg
    31801 attgccacct ggcttcccgc tgggctgcgc gaggtgacaa ccactgcttc ggcgctaagt
    31861 gattgccacc tggcgtcacg cagggttggg ggaggtggca actgctgctt cggcgctaag
    31921 tgattgccac ctggcttccc gctgggctgc gctaggtgac aaccactgct tcggcgctaa
    31981 gtgattgcca cctggcttcc cacagggctg cgcgaggtga caaccactgc ttcaacgcta
    32041 agtgattgcc accaggcttc ccgcagggct gggggaggtg acaaccactg cttcagcgct
    32101 aagcgattgc cacctggctt cccgcaggtc tacgctaggt gacaaccact gttcaacccc
    32161 gggcttcagc cccggggttt gatctcaagg gataaaatac gggctttagc cccggagagc
    32221 ggatcatcct gggctagcga ttaatcttaa aactccgtga actcccaccc tccgtgcgcc
    32281 caatcaacgc taagtgattg ccacctggct tcccgcaggg ctaagctagg tgacaaccac
    32341 tgctactgca ccactaccct tcaatcgctg ccactcctgg aagttgtttc ccttcaagca
    32401 actcgagcat tgctcctccg ccggtcgata cataactgac ctcgtcatcg agaccggcct
    32461 ggttaatggc ggctacggaa tcccctccgc cgatcaggga ataggctccc tcccgggtgg
    32521 catcggccac ggcctgggcc acaaaagcgg tgccccttgc aaaggaaggc atttcaaaaa
    32581 cgcccattgg gccgttccag agtatggttt tagcctgcag gaggacggtc ctgaattgtt
    32641 ccctggccac cggtccgata tcgagtccca tccagtccgc cgggatctca tggctgttgc
    32701 agacctgggt attggaactg tctgaaaagg aatcggcgat caccgaatcc tggggcaaca
    32761 acaaaagttt gtttttggcc tgggctttgt cgagcagggt tttggccaat tcgattttct
    32821 cggcttcgac cagggacttg ccggtttccc caccaagggc tttgataaag gtataagcca
    32881 tgccgccccc aatgatgatc tggtcggcga tgtccaggat gcgttcgatc agcaggattt
    32941 tatcggatac cttggcccct cccagtacgg ccgtgaaggg gtgttgagga tgatcggtca
    33001 cttttcgcgc attggtcagc tcagccgcca tcagaaagcc aaaggatttc cttgaggtat
    33061 cgaaacaatt ggagaccccg acattgctgg catgcgcccg gtgggcggtc ccaaaggcat
    33121 cattgacata gagatcggcc agggatgccc attcctcaga aagtccggga tcgcattttt
    33181 cttcacctgc ttcaaatcgt gtattttcca taagcaggac ttcgccggcc tggagagccc
    33241 cggccacgga cttgagttta tttccccgga gttcatcgca aaactgcacc ggcctgccca
    33301 aaagctccga aagatgcggc acgaggtgcc tcagcgtgaa tttttcctta tctatgctgc
    33361 catccggttt gagtttggcc tccggccgtc cgaggtggga aaagaggatc accgcaccac
    33421 catgcccgag gatatgattg atcgtaggca gggccgcacg gatgcgggta tcatcggtga
    33481 tcttgaagga tttgtccagg gggacattga agtcgacccg gacaagggct tttttattgg
    33541 agaagttgag ggattggaag gttgccatgt ggtggatggt ggatggtgga tggtgggtgg
    33601 tggatggtgg gtggtgggtt ctacgtccaa tcaccatcgg ccaaccacca aacacccgct
    33661 aaatcatttc cgcaaaatat ttcagtgtcc tgaccagctg gctgacataa ctcatctcat
    33721 tgtcatacca ggctacggtg cgcaccaact gggtatctcc aacgttttgc accttggtct
    33781 gggtggcatc gaagagggaa ccgaaggtca tgccgatcac atcagaactt acgatctgat
    33841 cgttggtata accataactt tcattggcgg cggctttcat ggcgttattg acttcttcgg
    33901 tgccggatac tttggttttc aggatgcagg tcaattcggt caatgagccg gtgagggtgg
    33961 ggaccctttg ggcggtgcca tccagtttgc ctttgaggct gggcaggacc aggccgatgg
    34021 cttttgcggc gccggtgctg ttgggtacga tattctgagc ggctgccctg gcccggcgaa
    34081 ggtcgccttt ggggtggggt gcatcctggg tgttctggtc attggtatag gcgtgaatcg
    34141 tggtcatcag gccatttacg atgccgtatt tttcttccag gacctgggcc atgggggcca
    34201 ggcaattggt ggtgcaggag gcgcaggaga tgatggtctc cgaaccgtcg aggagatggt
    34261 ggttgacgtt gtaaacaatg gttttgaggt caccggtggc cggggcggag atcaccactt
    34321 tgcgcgcgcc ggcggtgata tgggccgagg ctttatcctt gtctgtaaaa aagccggtgc
    34381 attccagcac gacgtccacg ccatggtctt tccacgggat ctgggagggg tctttctggg
    34441 catagacctt gatgatatcc ccgttgacca cgatgctgct ttcggcggcg ctcacctcgg
    34501 cgtggaacct gccctgggcg gtgtcgtatt tgaggaggtg tgccaggacg gcggggctgg
    34561 tgaggtcatt gaccgctacg acgtcgatcc ctttcatatc gtagatctgg cggtaaacca
    34621 ggcgtccgat gcggccaaat ccgttgattg ctacttttac ggtactcatg ttgattatga
    34681 tttattttta tttattcggg gctcaaaaat aggcagaggc ggcgggatgt ccgctataaa
    34741 aaagggactg aaatgtggat ttcatatgtt aacatttctt aaaatcaggg accattggcg
    34801 cgaataagca caaattaaaa ggtagcagca accatctttc cttgggcttg gttcccgttc
    34861 atttcagcca taagaaccta tccacgaaat caggtataag ccggcaggga tggaatgttt
    34921 tattataata tttgcctggc ttaataaagt cctttaaatc aaagatatat aagaatcccg
    34981 caagggtgaa ttattcccaa ataaaaagtc ctatgcaaac caggcattgc ctataccata
    35041 aattaactac ctaactcctg gtctcttaaa aaggaaataa attgttttaa gcctggctca
    35101 ttttgtctcg attgaagcgt tcccgtatac caggttctgt aaaatccatt tttacctatg
    35161 ggtatgagct taatcaaact tgtgtccctg acgaagggtt ttaccagcca cttggatagc
    35221 accgtcagac ccatacctgc tttcaccagt tccacccttg cttcagtgag ttgcattttg
    35281 gtgacgtgtc caatgggtat tgacctggga attaatacgt tctgggcaaa ataatcttta
    35341 ttaatatctt ctttgtagat gataaggctc tcgtttttga aatcagacgg tcgcaaatag
    35401 ggcctggcag ctaaaggatg cctggagggc accagggcca cctgttcatc ctcaaataat
    35461 ttttcaaact taatgccaga ttgccttttg aggttgttgg taatggccag atcgatttct
    35521 ccctttaaca aatagtctac cggattgctc atggcttccg tcacgatatc aatttcagta
    35581 gtcggaaatt ccgtctgaaa ttttttcatg atctccggaa gccaatggta acacgtataa
    35641 cattgcgtag tcagtcgcaa gtgcttcact tttcttctga ttccttttat atcgttttca
    35701 atttgtgcta tgcgcggaag aatttcatgt gccgcttcga ttattttctt tccttcctcg
    35761 gtcaggatca gctttttatt tatacgaaga aagaggcttg cgccaagctt tgattcaatt
    35821 tctttaagct ggtgactcag cgtgggctgc gtcaggaaca atttttcaga tgctttggtc
    35881 atgctgcccg cttccgaaat catttttata agctggaaat ggcgtatttc aatcattgaa
    35941 aaaatctatc gttttgataa aattaattca tttttagata tatgtcaatg acgctagatt
    36001 gaccggtaaa aatcatactt ccatgatcgc cttcctttta aaatcgtcca tccgcctgat
    36061 ccgcagacac cagtttctcg ccatattgaa gctgtccggc cttgcaatcg gcatggccac
    36121 ttttctgatt accgccttat ttgttattca tgaattgacc tttgaccgtc agcacccgga
    36181 tttccggaga atcttccgct atgttcaccg ggtcagctcc gacaataacc ttcagcaatt
    36241 tgccttcacc tcggccacga ccggccctgc cttaatcgaa cgcttccagg aggtggagtc
    36301 ctttgtccgg atttttaaaa gtgtggtcag ccttaagcgg aacgatgcgg acgtggggtt
    36361 tatcgaaaaa aaatttgcat ttgccgatac caatttcctg gaaatctttt ccttcccact
    36421 ccggcagggt tatgacaaag atgatatttt gaaagacccc tatgctctgc tactcactcc
    36481 cgacatggcg aagaaatact ttggccagca ggaccccatc ggtaagactt tagtcctcaa
    36541 tggtcagatt gagttgaccg ttaaaggcgt tttccgggaa aatttcagcc gctcgcattt
    36601 aaattttgac tttgtttctt ccttttccac cctcgatatt attaaaaata atcccattgt
    36661 ctctatacaa attccggctt cgctcaacct ggaaaacaaa ggttttgcag ctttttatac
    36721 gtatctcaaa cttcacagct ccgaccaggc cagtaccctt attggaaaat ttccagcttt
    36781 cattgaggaa ttccggggta agggcaggtc tgaacggctt aaaccaaccc ttcaaagtct
    36841 ggaaagcatt cacctccatt cggacatgct ctatgaaata gataaaaacg gctccctgaa
    36901 aatgatcctc atttatggca ttgtcggctt gctcatcctg gctgcggcga tcatcaatta
    36961 tgtaaacatc agcatggccg aatttaccat gcggtcaaaa ggagtaggct taaagaagat
    37021 ccttggaatt tccagaagtt ccctttggct cagtcatttt atggaaaccc tggtgctttg
    37081 tttcgggggt ctgctttcag gtggactcct ggccatgctg ctgatccccg gatttaacac
    37141 cctcatggaa accaggattt catttttcac tttcgagatg gttatcctgg cgggactgat
    37201 ttatgttgtt acctccattt tgtccggcag cctcccggcc tttcaaatgg gcggacaaaa
    37261 agccctggtt gcctttaaag ggagctttca aagtacaaaa acaggactga ttatccggaa
    37321 tgcgctggtg tttatacaac tgctggtttc ttttgtattg ttgacgatct ctatgctgat
    37381 tttcctgcaa attgactatt tacttcaaaa agacccgggc tttgcctcgg atcagatcat
    37441 cgtggtgaac gcatccagcc tttccttgga tgaacgtcgg tccattaaaa ccacactggg
    37501 gacggacaag aatgtcgttt ccacctccat gtgttctact ccacccgggg aacccttgtt
    37561 tacctttggt gcgacccttc ctggaaattc aggtgacgaa gaccggaggt tgacctttta
    37621 ccagatgttt gtggatgaag actttttgag tgccatggga ctgagtctga aagaaggaag
    37681 atttttcgat gggcgaattt ccgctgattc cgtgaactca ttcgttatca atgaagccgg
    37741 ggctaaagct attggtcatc aagcgatttc tcaaaggatc gaaataccga gtatgattcc
    37801 aggtgttcgc attaagaaaa acgtagtggg ggtcatccac gattttcatt tttcttcctt
    37861 tcactccgcc gtagagcctc ttcttcttga gttcaatccg caaaatgcag ggtatttaat
    37921 ggtacgattc aatccggtac aggcaaaaaa tgtgattgcg gccattgaac aagcctggaa
    37981 ggccagtgcc gcccgcctgc cttttaatta ttattttttg gacgatggat ttgcccggta
    38041 ttatgcagca gaacaaagaa caaaaaaaat cgttgccctg gttaccctgc tgaccgtttt
    38101 cctggcctcc ctcggaattt ttggtacctc gttgtttacc atgcagcagc gatcaaaaga
    38161 gatgagtttg agaaaactgc tgggttccac cgccattaat ttatttgccc tcgtattcag
    38221 accattcctg cttggactgg tatgggcaag cctggttggc gctcctatgg ccgtttggat
    38281 cggtaacgaa tggctgagga attatcctta tcatacctcg ttttcttacc ttctcccggt
    38341 aatttctttc ggactgatcg cgataattat ttttataacc cttttgtttt atttattcag
    38401 aataattaaa atgcagcctg ccgtggtttt aagggagagt aattaagttg gccatagcga
    38461 atgttattca tcagccatgg taaattattt aaattactga ttaaggacgg ctgaatgatg
    38521 ggatgtcacc cggtatattg gcaagctata ggttggccct attttttaca ttcgcttagg
    38581 tcagcggacg cgaatccctc aaccattttc caattacgga ttgaccattt tcctgtttct
    38641 caacgagaca attactaagg caccaaaaat aattgctatg attgcatgcg tcagccaatt
    38701 gtttaccaca ttacggatgt caaaagattt gttgccggtt tcccaataag tccaaacgtc
    38761 ggaaatctca gttattaaac cttgcgtgcc cgaagcaagc agcacaattc catttcccgt
    38821 cgccggattc acacgcattt ctgctcctga agccggaaaa gcgccacccc cgtgcccaaa
    38881 aacatatcct ccgctgttat tttcaccata aagggtgtga cctaatcccc aagtgaggga
    38941 agttccgggt tgaggcgtgc caaattcttt tattgactct ttagttaaca ctgcattggt
    39001 attgtaatag gccctcagca attgcgcaag gtcatgggct gtagaacgaa gcgaaacacc
    39061 ggccatattg gtatagcggc gatgagggta cgttttaaga ttcaggtcat agttggaagc
    39121 caaatgatgt tttctgccct gagctaatat ggaatccaaa gaataacagg atttggtcat
    39181 tttaagcgga aggaaaacct tttcttgcat gtaatcatta aacgcttgcc ctgatatttc
    39241 ctctatcagt aattgcagaa ctgcataacc tgcactggaa taggacatca ccgtcccagg
    39301 ctctctgact atcagcgctg catgaggctg ccccatatta acatctttgg gaaaggtgag
    39361 ggattcttca atggtttgca attcttcgcc tggtaaaaat ccaccgtgtc catagttatc
    39421 ctcaaagcct gccgtatgat tcagaagatg tcggacggtt accctttgcc ggtaattttc
    39481 gctgccggga aattgccaac gtgttaaaaa gggtaatacc ggctcatcca aaccaatttt
    39541 tttttcttgg accaacttca ttatacccca tgcggtaacg gccttgctta atgaggaaag
    39601 caggtacaac gtctcctcag tgtttaccgg tgtgtttgtc tcgaaatggg ctactccaaa
    39661 accttgttcg gttattatgc ggccattttg aacaaggaca agtgccgcgc tgcccaattt
    39721 tttatcggct tgagcatttt tcaaccggtg taccacataa ttttcaatag agacaaggtc
    39781 tccttttact acgggtggtt ttttaagaat ctcccaactg atgaaaaacg cgattaccaa
    39841 agccgcccat aaaaatatgc aggctgtcgt gattgccggt ttacgcattt ttagtacgat
    39901 gtcaaatcct tgtttattat tattgccaaa ttactgccaa tgtcttgcta tttaaagtgg
    39961 cggagatggg aggtctaaaa aatggagcgt agcggaatga taagcgcgac cttcacgtca
    40021 ctgtcgacct tgaacaaacg tgatttgaaa ataaagctac aaatttacac cgaacccccc
    40081 cattgcaaat agctgcattt tgtggctgtg cggctttctc ctccgttcat tcgaagcctg
    40141 gtcattcgtc acccgctgaa ttttgttttc attgcattta tgattagaaa aattgagttt
    40201 gttaatcgca ttccagtcca cttgggaaat tctttggttg cttaaagatt aaaaaatgtt
    40261 tgacagacaa aataaaaaac cctctaaatc aatgatttag agggttttga agtggcccgt
    40321 acgggattcc ccgcctgacc gacctgttga catcttactc aggtcagtcg ggcaggtaac
    40381 ccgtgtttcc tccgtaatta atcctatttt atagccacct ctttgcagcg ggcagttttc
    40441 agatattttt ccctgatcct accggtttgc caatcaccac aggttactac gaaaattagc
    40501 ttccagggtc tgtggcatct tgtaattcta tttttaccat tattgtgctc aatcaaactc
    40561 cccctgcatt aattgtcaac ccagtatagt atagtataac ggctgtttgt agttaaattt
    40621 gacagcaaat aatagtatca gacccatgca gggaaaacaa ctcagaaaat tagaagcaga
    40681 actctggagg gcagccgacc agctccgggc aaacagtaaa ctgacggctt cggaatactc
    40741 catgcccgtc ctgggtctca tctttttgag gcatgccttt aaccgatatc agaaagtaaa
    40801 aacggaagtg gagaaagacc tgccctccca tccccagcgg ggaaagaggc ctttgaccaa
    40861 aaaagacttt gaagcccgga actccatgtt tttaccggaa aaagcacaat ttgactatct
    40921 ggtgtcactg cctgaaagcg cagacattgg ggacgccatc gaccaggcca tgaaatccat
    40981 tgaggacgaa tacgacaacc tcaaaggcgt gttgccaaag aacttttcta ttttcagcaa
    41041 agacctgctg aaagaactgt tgcggatatt caataaagaa gtattgcaga aagccgaagg
    41101 cgatctgttt ggtaagattt acgaatattt cctcggcaaa tttgccatga ccggcgccca
    41161 ggaaggggga gagtttttta cgcccatgag tctagtcaat accatcgtca acgtcatcga
    41221 acccgaccac ggtatagtat tcgatccggc ttgcggctca gcgggtatgt ttgtgcagac
    41281 cggctatttt atagaaagtg aagggttgag gcctgctgaa aaagtcagtt tttacgggca
    41341 ggaaaaagcc gaactcaata ccaaactggc taaaatgaac ctggccgtac atggactgga
    41401 aggtaatatc caggaaggaa acaccttcta cgaagacaaa cacaatctgg tccaccgggc
    41461 tgattttgtg atggccaatc ctccttttaa tgtcgatggg gtggacaaag caaaagagat
    41521 cgtgaaaaag gacccccgcc tgatgctgga cggtaaagta tatctgccca aaaatgacaa
    41581 tgcaaattat ctctggatca cctatttcta caattacctc aaacccacgg gcagggcagg
    41641 ttttgtcatg gcttcttcag cgagtgacgc cggccacagt gaaaaggaaa tccgggaaaa
    41701 actggtcaaa acaggggcag tagatgtgat gatggctatc ggaaacaact ttttttatac
    41761 ccgatccctg ccctgtacct tatggttttt tgacagggct aaggaaaagg acaaggagaa
    41821 aaaggataaa gtcctgatgc tggatgcgcg taaaatctac cgcaaagtaa ccagcaaggt
    41881 aaacgacttc agcgaagaac aattgcagaa tttgatttgt atcgtcaacc tgtaccgggg
    41941 caatacccaa aagttaaaat caaccataaa atactatttg gaaactgctg ctgcccttgc
    42001 caaggagacc gccgatgcaa cgatggaatt acaaaaacaa ctgcagctcg tgcataaaac
    42061 agtaagcgga tttgccacca aatacgcccg ggagaataaa gaagcgaaaa attttgttga
    42121 ttcattgaaa tttgaagaaa cggcaagctt ttatgagcaa caagataaac tgattgatgc
    42181 gattataaat gtaaaagccg atatcacctc cttagaaaat atcgcccacc aatgcaaaac
    42241 cctgcgcaaa ccccaggaca aactcatcaa acaattactt gacgtcatat cctcagccgt
    42301 caaagaattt caactcaata aaaataaaga ctggaaagag ctgaacctga aagaacaagt
    42361 cgaccaactg aaagccctgc aacagcaact cagcggcaac cccgatgaag aagaaccagg
    42421 cctattgcat gagaccgaat atttctggaa acaagcctat tggctgcaaa gtcggtttcc
    42481 cgatggagtt tataccgatg tggaaggctt atgcaaaata gtaagccaga aagaaataga
    42541 agccaatgac tggagcctga gcccaggcag atatgtaggc gtggataacg ccacagatga
    42601 tgattttgat tatgaagaaa gacttaatga aatacatatt gaattggaag gattgaacga
    42661 agaagcaatt gcattgtcga atgcaattgc agagaatttt aaacagatag tgctatgagt
    42721 tatcgaatag aaaaaatgaa ggatgcacca ttgaaattta tagatggtga taggtcttcg
    42781 aaatatccga aaagaaatga atttcaatca gaagggataa tgtttcttaa tgccaatagt
    42841 atatcaagtg gtattttcga tattagtgca gtgaattata tttcccaaga aaaatatgaa
    42901 acaattacaa agggaagaat agtgccaaat gatattattt taactacaag aggaaatggt
    42961 gttggagata gttttctttt taaagaaaag tatggtgaag gccttattaa tgctcaaatg
    43021 ctaattatca gatgtgatga aaaaatgtta tccccacaat atttatatta ttttttcacc
    43081 aaccctgctt gcaaaaatta ctttataaat tgttcttctg gttcagcaca acctcaatta
    43141 ccaataaaga gtttaaagga aatagaaatt aaataccccc ctctccccac ccaacgccgc
    43201 atcgcctcca ttctatcggc ttatgatgat ctgatagaga ataatttgaa gcggataaaa
    43261 ttgttggagg agaaggcgtt tttgaggtat aagggaattg tgaggaatga gaagatggag
    43321 gagttaacaa ttgaagattt ggcaaatgtt gtaatgggac aaagtccaat ttctgatact
    43381 tttacagatg aaaaaaatga tataccattt catcaaggtg ttacaaattt tactaactac
    43441 tttgtcgagc ataaaattta ttgcaattct ccaattaaaa ttgccgaagc aggcgatata
    43501 cttttttcag taagggcacc tgttggaaga ataaatttta caactgataa aatttgtata
    43561 ggtcggggct tgtgtgcttt ccgaaataaa aaaggatttc aaaacttttt ttatcaccag
    43621 ttgaaagaat ttttttttac tgaagatgta attggaaatg gtgcaatttt taatgcagta
    43681 accaaacttg agttaatgaa agtgaaattt aaaactccaa gtgaaaaaat tcaactagaa
    43741 tttgaaaatg aagctaaggt gatctataac ttaattttaa accttactat gcaaaacgcc
    43801 aaactcatgg aagcaagaga catattatta ccccggttaa tgaatgggga gatcgaggtt
    43861 tgaaatattt attatcaata aaatatacat tagttaaatg taaatatgat acaaattcac
    43921 ttttaagtga aacaaaatgt ataaaattca gttataagtg aatagattta gcctggaaat
    43981 atgggatgat gaaggacgca tatgcacatt ctacaccgtc aggtgggtta cagttaatga
    44041 tgatacatca tccgaaacgg acaggttttt tgatacctat gccatgccta cgcatccact
    44101 tgaagaaagg gcaatgcaat tgtttcgatt gataactgaa agcatcggta atagatacgg
    44161 agcaaccgat gatttttttg acagaattgt aaataaggca caagaattgc caccaaaacc
    44221 aaaacaatgg gtagaagaaa taaaagactt aggcatcaat ttcccactga ggttattttg
    44281 ttatcgggta accgaaaaca ttgttattct ttttaatggt gggattaagg aatctcaagc
    44341 aacacaaaaa agcaaagatt taagtatgaa gttttacgaa gcccaaactt tcgtcaaaaa
    44401 aattgaagag gcattgcgaa ctaaaatgat tgaaatctcg gatgacgaaa ggcatttgca
    44461 aaactttgac ggaacaacag atataatatt ttaagacaat gagaagtaaa gttgtagata
    44521 gattgctgaa aagcattcct aaggatgtag aaatctttgt ggattggtat gccgacctgg
    44581 tagtgcgcat caaccagtta ttgcatgaaa agaatatctc aaaaaaggaa ttagcggaaa
    44641 agcttgacaa aaatccatct gaaatttcca aatggctgag tggtgagcac aatttcacgc
    44701 tgcgttcact ggcaaaattg tcggctgaat tgggtgagcc tttgttggag gtacctaaga
    44761 aaaaagcaca gacagaattt gtggaaggtg gttttatatg cagggtgcac acttttgtga
    44821 gttatacaaa aatcgatgcg aaaaccactg ataaggtatg gcaattagcc gaagaaattg
    44881 aaccctacaa agcattgagc aatgccggat aaaaatatat ttgacccgga aaaaatcacc
    44941 ctgatagatt tcaagatgat aaaaggtcag gtggatacgc cagagaactt tgacaatagc
    45001 aaggtgtcag gacatcaatt agacaattct ttgaagctgg catttaacct tgttgacaaa
    45061 ttggcaaagg ttgattttac gcttacaata aaaacggata gcaaaggcga aaatgaaagt
    45121 gaagcaaccg gaaattttca tttgattttc atttatcgga ttgaaaattt ggcggaactt
    45181 gctatacccg aaaagaataa acgactgaat ttggatgcgg gattggccaa tgctctttca
    45241 tccgttacct attccacttc aagaggaatt cttttgacca gacttcaagg caccgcttta
    45301 caagattttg tattgcccat tatcaaccca aacaagttgc ttcagaataa gtaaaaagga
    45361 atgacttggg aatactcaga agataattta attgaaaaaa ccgctattga tttatttttt
    45421 aatcaattgg gatgggatac agctattgca ttcaacaagg aaacctttgg agaaggcagt
    45481 accctgggcc ggttgaataa aaaagaggtg atcctgaagc gcatcttttt tgatagactc
    45541 agggacttga atcccggatt accggacctg gcctatcaac aagcttatga aaaactaagc
    45601 actgatagtg tcaccagatc cttagcagaa atcaattatg aaaaatacca gtccctgcga
    45661 gaaggcatcc cggttgactt tgtcaatgac cggggtgaac aagtcaaaaa caaaaccttg
    45721 gcggtatttg attttgacaa gccggaaacc aataattttt tagcggtacg gcaattgtgg
    45781 gttcagggca aaagcaaccg ggaacgcaga ccggatatta tcggttttgt caatggcatc
    45841 cctttgcttt ttattgaact caaggcagcc caccgtaagc tggaaaatgc cttcaacgat
    45901 aattttacgg actataaaga cgtcattccc aacttgtttt attacaatgc atttgtagtc
    45961 ttaagtaatg gaatagaaag cagggtgggc agcattacgg gtaaatacca gcactttcat
    46021 gaatggaaaa gaatttcgga agaggacgaa ggcgtggtag ccctggacag gatcataaag
    46081 ggcatctgca ataagaagcg gtttatggat ctttttgaaa actttatcct gtatgatcat
    46141 tccctgggca aagtagtaaa actgatcgcc cgcaaccatc agtttatcgg ggtaaataaa
    46201 gccattgaaa acattaagca aaaaattctt ctatacgccg aaggcaaaat aagcctggaa
    46261 gagaagcaga aattgggggt attttggcat acccagggaa gcggaaaatc ctattcgatg
    46321 gtattctttg gccagaagat tcaccgcaag tttaccggca gttatacctt cctgattgtc
    46381 acggatcgca acgaattaga taatcagatc tatggcacct tcagcggagt aggtgtgata
    46441 ccccaggtta aagcaggttc cagggattcg ctgaaagctg gcagcggcca gcatttgagg
    46501 gaattactgg gctcggacca ccgctattta tttaccctca ttcataaatt taattttgaa
    46561 gacgtcatca ctccccggga taatattatt gtgatttccg atgaagcgca tagaacccag
    46621 ggtggaacgc tggcgatgaa tttgcgcaat gccctgccca atgcttcttt tataggattt
    46681 accgggaccc cgctgtttaa agatgatgaa ttgacgaaac ggatatttgg agattatgtg
    46741 tccaaatatg attttcaaag gagcgtggat gacggggcca cggtacctct gtattatgaa
    46801 aaccggggag aatacctggg tttaaaaaat ccggccatca atgaacaaat cagggctgtg
    46861 ctggaccagg agactgaaga tttagacagc gaccaaagaa gtaagataga gcaacttttt
    46921 gcgagagagt accccatcct gaccaccagg aaaaggttgg aggccatagc caaagacgtg
    46981 gtatggcatt tctgcaaccg gggatataaa ggcaagggca tgtttattgc cctggataaa
    47041 ctgaccgcgg tgcgcatgta cgatctgatc ctgcatcact ggaaattata tgtggaacaa
    47101 ttggagaagg aaatatccaa agcaaactat ggtgaccagg aataccagga caaaagccga
    47161 gaattgaaat ggatcaaaga aaccgagatc tgtgttgtag tcagcgggga acagaatgaa
    47221 atccagaaat tccgggcatg ggatctcgac atcgaggccc accgtgaaaa aatgaataca
    47281 caggatctgg agacccggtt
//
LOCUS       DAOQXT010000026        46189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_892_length_46189_cov_6.197750, whole genome shotgun sequence.
ACCESSION   DAOQXT010000026 DAOQXT010000000
VERSION     DAOQXT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 46189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46189
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_46189_cov_6.197750"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            674..3916
                     /locus_tag="PKM27_06505"
     CDS             674..3916
                     /locus_tag="PKM27_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNR06951.1"
                     /translation="MKVYLMWILCIGLGFTAQAQRRISGIISDQNGDPLIGVNILVQG
                     TNNGTVSDENGAYSIEVRPENRVLVFSYTGFTSREIEIGTASTLDVTLTEGVLLDEMV
                     VTALGITREKKEIGYAIEEIKGEEIQRVGETDPLRAMQGKIAGVNINASSSQPGSATR
                     LTIRGLKSFFGNNQALYVVDGIPFDATTNGVSFQLTGGGAYANRLADLDPNNIESVSV
                     LKGATAAALYGSRASRGVVVITTKTGTIKKGENRMKVELTSGLNFEKIASLPDFQNKY
                     GTGTGFLYQQANGSWGAPFKGTVPYPTIDSINHWYLGAGYAASFNRLVQSGDPRVTQK
                     VPYQAYPNNVKDVFETGRLMDNSITISGGNQNTNMALTFSRVDHAGIFPNSNFDKTSL
                     SLGANSNFESGLRMGANLSYVNSIQEGLQGGAIGSVSTSSFMARTLYQGRNWDLQGQP
                     YEDPLTRESLFMVARGQADNPYWSARYNGFESNVNRFVVNVNAGYTLSKWLDVDYRAG
                     LNQYNQGDLEWFRPGSRGAGGLGSVTDLNTNFREIESTLIFTAKHRFNDSWAIEARAG
                     HNLNERTSKAQGISGTQMLDFNILDVDNTVNQVSAGGNFSKQRLFGLFGEFTTDYKQY
                     LFLTLSGRNDWSSTLPVKNNNYFYPSASVAFIFSEAFNMINNPILNFGKIRMNWSKVG
                     SPAAPYSLKPIYLLNLGEPDNLTAGIRDIDFPFAGIASSTLSNTLFDPDLKPEFTTDL
                     EAGLDLEFLKGRIELNVSVFKSTSVDQISQLALPTVSGYDQLLTNFGALQTKGSEVSL
                     RLNPISKKNFNWNIITNFTRYKTIVTELREGTEEMVIRPLFAGGITPVLRVGQPYGIL
                     KGTVSARDDEGNLLIDKANGQIINGIDDAIVGDPNPDFTVGIVNSISWKGLSLGAVFD
                     WRQGGDLYSETVNALLGRGVLNFQADREKNAVIPGVYGDPVTLKPILDEAGRSIRNQT
                     MVETNDLWFGSTFGTNSQDEWSVFDATVYRLRELSLSYSLPSSLFKKGIRGVTLSLIG
                     RNLWYKAPHFPEGTNLDPETSTFGDSNYQGFEFQNLPSIKRFGASINLKF"
     gene            3934..5541
                     /locus_tag="PKM27_06510"
     CDS             3934..5541
                     /locus_tag="PKM27_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNR06952.1"
                     /translation="MKFYRLIFLICGFSLISISSCDDYLDINDDPNNPTDARVNNLMP
                     FSQAVVFGSLGIGTAGISELVSVYSHHTVQRGNHDDYKITANEFALTQAWEKMYAVAL
                     PDLDIIIQKATEANNFAYVGMAKLLKVQIFSSVVDLWGDMPYSEAALGAEAPFPKYDD
                     DKEIYRDLFRLGFEALADLDKPSTLKPTTDDLVYKGNMDKWKKYGRSLLLNMYNKARL
                     TDLYDAAKVAELLKLSHISGTSEDFELAYTTSITPENRNPGFVREYTLGNPTYYISPY
                     FYLMMKGEAACENTLLEGIADPRIPYFFYNQLGPNEQAENPVSYQDGSFLSIWFGSYD
                     KDPNEGFDQGKSQTLLGLWPVGGAYDNGSGANGDLNSGLKGAGAQRLLTASDVNFIRA
                     ELALTKNTGEDAKALLNTGIRSSFAKVNAVARLAGAPVIPDAAITTYVDKVLALFDAA
                     DNARKLELILTQKWIHSFGNGLESYNDIRRTGFPKICDPAKDPNVHSSQSRPYPVTLP
                     YNGDDLQTNPNAKQHNQYTDKVFWDVD"
     gene            5584..6486
                     /locus_tag="PKM27_06515"
     CDS             5584..6486
                     /locus_tag="PKM27_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06953.1"
                     /translation="MKKILIYIPALLLLVRCTDESKIRIPEIANGVNLRIVVDPAHTG
                     IFTDKIATDFLAFDLYSENKDLASVQIFAATGGVRKLAKSLTQSDFDQGQGKVRMELK
                     ASDFATLFGEPGYGDGSKVANFTFNPQVTLNDGRVYPSYVKLSATDSVLNLAPSINGA
                     AASSFTLQFQSFITCPAVDISGDYKVEDATGSSTDGCCPTETTVKGIVVKITKLTASS
                     FKLSDFSGGLYFEWYDVYGITKPEDSPGEIAFSCNEVFFQNTKEPFGENVSGSGDYDP
                     ATKTLVYHWVNGYGDEGTVKLVKQ"
     gene            complement(6735..7424)
                     /locus_tag="PKM27_06520"
     CDS             complement(6735..7424)
                     /locus_tag="PKM27_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06954.1"
                     /translation="MLKFTKIGVLGLMLLTIPLSGQQQTANCRVLSAKLQGTYQGECK
                     NRLAHGEGTAQGTDTYQGHFKEGVPHGKGKYVYSDGSYYIGEFKNGLRHGEGTLHTMD
                     KETSTTQSGKLSLWKDDEFIEEILEKKYKIIQSLNTLSTQFDKPNEIQDRVEIFLGTR
                     SMINGINIFASTGNHYVQGKNRIIIENVSFPLLVRLNYTSQKGVSEVLPVRVEFELES
                     KGHWKINVNSQ"
     gene            7605..10814
                     /locus_tag="PKM27_06525"
     CDS             7605..10814
                     /locus_tag="PKM27_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNR06955.1"
                     /translation="MMKKLTLTFATFLFAVAIGLAQRTITGTVKEVSGEPLIGANVYV
                     KGTTTGTTTDIDGTYSLEVPSDASILVFSYIGYTDQEVTVGNQSTIDVTLAEGITLQE
                     TVVTALGISRSQKSLTYSAQQVDGDQLRQIPATSVNNALSGKVAGIQVRSQSTVALGR
                     DAAIRIRGAGSLNDKQPLYVVDGTPVASAQDISVDDIAAMTVLKGPSATALYGQRGDA
                     GVIVIQTKKANNKQALGIEINSGTFFDQVYVLPRYQNSYAGGGAADLIEFNWKEGMPD
                     EWKIFEGKYHHDYSDDASWGPRMVGQEYIPWYAWYVGTPAFGQTAKLVPQPNNIRDFY
                     QTGVTLNNNVSLSKGGEGYNVRLSYTNQNVKGLLPSSSANKNTINTSASFDLGKYLTA
                     STNINVSNWIVNGVFDDGYANQSSGSFNQWFHRHLDLNKIKEYKGMKSPEGILSSWNH
                     NNPGNYLTSPLSFYGGNYWYNFYDFFENIDQSSRRTRLFGDVSLTFKPFKDLTVQGWV
                     RRNQIEVVNEGFGRAILENSATQTGFKSYYFYNQGYYEPSDGSPGKEDNYEALASYHK
                     RFGDLSLDVKAGGNIRKVQFSQISGSTVDGLSVPDLFTLANSRTTPITFGSGRSFKRV
                     NSLYGTGTLGYKDFLYVDFAARNDWSSALPKDANSYFYPSVGVSFLFSELLGIHDLSF
                     GKLRGSWAQVGSDLGAYQLALNYGVNSNQWNGAFLMGTPDALVDPNIKPSLSSAYEGG
                     LDLKFFSNRLGLSGTYYKEKKIDEILNVPVSATSGFASKNINAGRVDRSGVELTLEVT
                     PVSNRNVNWNMTFNWARNRTKIIELAEGINSIIRETGTFGTSSGARLVHQVGELWGQV
                     RGGGIKRNDAGVPVLDASGQFINVPDTYFGSVLPDFTGGILNEISVGNLFANFNIDFS
                     KGGVFYSLSDHWGKFSGLFEETAALNDKGNPSRDPVAVGGGVHVVGVDAEGKPVDTYV
                     DAQTYWHQFRNKSIAEDNIHPLDFVKLREVNIGYKVNTNKLGIPAVKNLVVSFIARNP
                     WLISSKTKDFDPSEISQSYGENGQFPGTRSFGFNVKVGF"
     gene            10826..12283
                     /locus_tag="PKM27_06530"
     CDS             10826..12283
                     /locus_tag="PKM27_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNR06956.1"
                     /translation="MKNSFKSILIVAAALLFSTCSLSDFGDLNRNPNATTVPVTSALL
                     TNALAGLGGWTQGMNEALYCQYFSETQYTDASLYSLIQVNFTGNYSGAMYDLQNIIDN
                     NTNPDTKDEVLANGSNANQIAVARILLVYHFMHQTDRFGDVPYSEALKGDAQPAYDTQ
                     LDIYKGFYKELKEAIAQFDNGLAAQGDILFDGDKNKWKKFANSLRLILAMRLSKADAN
                     LGKAEFAAAFSDPAGYLSSASEDVVVHYPGAAFKNPWFTLYDGRKDYSISDVMVERLK
                     SWNDPRLPVLGNPNAQGAVKGVPYGLTRDAAIAFTNANADWSQILAPKWRAQNSPLVV
                     LSASQVLLTIAEAIHLGWVTGDKAAFYANGIKASWERWEVYNEQRFLGYIQPTGIGTE
                     LIALTGGPSDLEKINLQKWVAIYPDGRSGWTEWRRTNTPVLTPTPAAVSNSKQIPRRF
                     TYPSVEYNLNRENLDAALGRLGGPDNQDGRVWWDK"
     gene            12319..13260
                     /locus_tag="PKM27_06535"
     CDS             12319..13260
                     /locus_tag="PKM27_06535"
                     /inference="COORDINATES: protein motif:HMM:NF020111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HNR06957.1"
                     /translation="MKLKYIMGLLVLASFTSCIEEGENAIEGIGGNFVRIAGGGDEVK
                     AIGFSARPGSATLPLFTVQRDANSEANLNTSAEVTLALDPAMIAAYNDANGTEFVDMP
                     ANLYNLKSLNLAFAPGEFAKKVTITVDPSTFDLSKAYAIGIKVASVSNGYTIVESAKS
                     GLFNILVKNDYDGIYKVTGTMTDYVSSALTGYFPMNYHLVTSGAVTVDGYDPDVWHAP
                     FVPIYSGTAVSGYGSFCPVFTFDPATNKIISVTNYYGQPAGNTRAAVIDPSGINKFNA
                     DDHSIDVKFFMTQTNAVAAPPHIRVAFDWHMEYLGPR"
     gene            13410..16490
                     /locus_tag="PKM27_06540"
     CDS             13410..16490
                     /locus_tag="PKM27_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR06958.1"
                     /translation="MKRLCQLGLSAFLFLFAFTGLHAQSVKGLVRAKADNQPLIGVSI
                     SVKGTNTGTVTDIDGAYEIAAGPNDVLVFTYIGFATAEVAVNGQAVVDVILSEDVKTI
                     EEVIVTGYGTQIKRDLTGNIAKIKASEIRDVPVNSVEAVLQGKAAGVQINAGTGKLGQ
                     AQQIRIRGNSSVSASNEPLFVVDGIPITTADLSNNGGGSTNSIVDINPQDIESIEILK
                     DASAAAIYGSRASNGVVLITTKKGREGRTNVNVGMQYGNSKPTRKVDFFNSAQFIDYY
                     RKAAANSDRIESIDPSDPDSYTTYMEGFFEGVSLGTYGTPNQADTRWDDLAFQDAPQT
                     QIDLSIDGGNAKTRFFVSGQFLDQKGIMVDNKLKRMTGRINLDHQVSDIFNLGFNLGL
                     SRTINERLDGDNAFSNPLQLVALTPLSPQFDPTTGLLIGSPPGDIGQPLYYNGLLNVD
                     GALFNTNVHRNLSNVYGALRIIPGLVFRSELGIDFLNQQEESYNSSLSQRNTGFPLGT
                     GSNYFTRVENLNTNNYFNYLKEFGSHKIDFTLGMSYQQSEQDFNSVTGTDYPSDSYKK
                     IASAAKITAGTSTETNFRFVSYFARANYKLSDKYLIGLSARVDGSSRFGSDSRYGFFP
                     AVSAGWILSEEDFLSDSRLVSFLKLRASYGRTGNAEIGNFPQLGLFQGDAGYGNQPGQ
                     RPAQLGNPNLKWETTDQMDIGLDFGLFNDRLNGEIDYYNKQTDGLLLNVNVPASTGFS
                     TQTKNVGKLENSGIEFALNGTVVNGRDFKWNIGVNYARNKNLINDLQGQIIEGGLSNM
                     SRAMEGQPLSTFFTVEYAGVDPANGDALWYKNSKNTDGTIDRGTTNRYNQAQRVVIGN
                     GLPKWVGGLTTSISFKGLDFNMLWNTVQGNLVNFYGAGRFSSANGRFEDNQTVDQLNS
                     WTPQNTNTNIPEARLFYNNGAQASSRFIQDGSFVRLRNATLGYSLPAVWLKNVGIQSA
                     RVYVTGLNLLTITDYTGWDPEVNSDDFVTNVAQGYDFYSPPLPRTIVGGLNIKF"
     gene            16513..17856
                     /locus_tag="PKM27_06545"
     CDS             16513..17856
                     /locus_tag="PKM27_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR06959.1"
                     /translation="MKKSIYIFLIFIFAACDKELDVKPTQSIDETNALATAQDVKVTL
                     TGAYDGLADGDVYGGGFQFTSELVADDREVRFIGTFAGQDEMWRKAMTTGNAQVLATW
                     RDAYVAINRANNVLSALDKLDAADKSQVEGEARLIRGATYLGLINLFAKAWGDGDQNN
                     NPGVPLVTTPTRVVTDEDNRPRATVAAVYAQILEDFTKAETLIPESADQNGFGTRNAA
                     TALLARTYLIQGNFAAARDAAHKVIASGSHALAESYVDVFYDKSGSFDGESIFKVIIT
                     EQDGVNSMNTYFAPATFAGRGDIVIQTKHTDLYGPNDPRGQFTVTTSRGVFTGKHLDQ
                     FGDISIIRLAEMYLIRAETNFRLGTVVGASPLEDLNAIRDRAGAGLLTLDQINLDRIL
                     LDRKLELMFEGQLLQDVRRLKKNVGTLPFSDPKMVLPIPQREIDTNKSLVQNSGY"
     gene            18081..21254
                     /locus_tag="PKM27_06550"
     CDS             18081..21254
                     /locus_tag="PKM27_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNR06960.1"
                     /translation="MKRLILLFILLITVSTAFTQRTIKGMVTDEAGEALIGANVVAKG
                     SSIGTVTDVDGAYSIIVPENVTELTFSYTGFNPKDVSIGTLSEINVILEEGVSIQEVV
                     VTSFGIKKDKSNLGYNVSQLNSEELTMAHTTNITNAISAKVPGVRTSGAGGSFASSSI
                     IIRGFTSFTGSNQPLFVVDGIAIDNTGGGSALQNGVTNSSRAIDLNQDDIESISVLKG
                     AAATSLYGSRGANGIILITTKSGKVRTKQNISYNLTYANQEINRLPDYQNEYAAGNAG
                     AYNPNGLSSWGPRIDGRQVLLPTVYRGLNGPGDTSMTLQAYPNNVKDLFRSAPNMQHN
                     LSFQGGKENYSYRLSAGYLDDKWIMDQNRLKRYNIGVNANNSITKKLTAGVSANYIRN
                     TSVRTPQGNQLANPLFRAWFTPRSWDLTGRPFEHPVTLNNLDWGSGAHDNPYWTIKNN
                     LYNDEVDRIFGNFNLRYDISKMLSASVKVGTDNFTYSASLYDQIGSTGGAGTSAGGRG
                     AIRDIRTVSRILNTTTLLTAQKDIGDFDLTLVLGNEILDQFRSDAQVTGYGVTVRNFR
                     NLNANTTTYFPVFNKWNYRLVGNFANFTAGYKNWGSIDLSARNDQNSILPQSNNSYYY
                     YSAAIKLNIMKMFALNSQFLSDFAILANTGLIGNAKPEFRYATDSYYGGAGAADGFGP
                     NIAFPFNSLSGFTLQNGAGNPDLKPEFTRSSEIGVNLGFWEDRIGIEVSAYQQKSTDI
                     ILSVPNSSAAGISSIIRNAGVLKTKGFEGILNISPIRSKDFQWNASVNYTQFKSTVDQ
                     LAPGVQNIFLGGFTTPNIRLVVGDEYGQIYGSDYNRTNDPTGKLFDPRLPYNPNGTVV
                     IGANGLPSATSGVQKIGNPNPKFLMGINNELKYKGLSLSVLLDIKEGGDQYSRNLADI
                     QRNGVGIETAEFDRFNSDGTLAKPYIFEGVKADGTPNNVAVTAEQYWGNSGKFVAAKG
                     FIYNTSWFRVREAALSYTFSRGLLDRTPFGSLIVGVFGRNLFLSAPDYPHLDPEQNAL
                     GVSNAQGLEFNALPQARSLGVNLSVTF"
     gene            21300..22817
                     /locus_tag="PKM27_06555"
     CDS             21300..22817
                     /locus_tag="PKM27_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNR06961.1"
                     /translation="MKTNKYIKLSLLLAVMLFSTVSCKDYLDINQDPNAVLNAPIEQV
                     LTSATVAFGFWAGSDMNRYGALIAQQWSGQTAGATVQTQDYEKYLITGSDVNNVWNLM
                     YATILSDLELVIQKAGEEGSPHYAGVAKVLKAYVFANIADTWGKAPFTEALKFTANSQ
                     PNYDDASVIYPALIAMLTEAVSDMNAASQKSPGTNSTIYSGSWSASRAKWIAAANTLR
                     MRLWLHTTKVDRAKAVSEIAAIAATGPVINAVADNFQMAFVNATNAQNPIQQFEIFRS
                     NYLFANATLVDMMNAKEDPRRWRYFTSFPLHSGNFKGAKSGDPSSQQYSRTHIYLRGD
                     TLNTATAASNGSIAFNAYTYSGTAPVRFLTAAESFFIRAEAAVLSGGNLGGLSADSLF
                     RQGIRMSMSAAGVSTAQAEAYIAQQGSLPGTEAENVKKIIEEKYVGNFGVINEPWADW
                     RRTGYPSQIVKVPNAVVTSSPDVPRSFFYPQSEIDLNPNAPPQKVNMVERVFWDK"
     gene            23130..26300
                     /locus_tag="PKM27_06560"
     CDS             23130..26300
                     /locus_tag="PKM27_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNR06962.1"
                     /translation="MKRLFLFVCLLGSAISAFGQRTISGKVTDSGGEALIGANVVAKG
                     TEIGTVTDIDGSYSINVPGEVTILTFTYTGFTPKDVVIGNASELNVIMEEGVAIQEVV
                     VTALGIQREKRALGYSVTTVDKKLIEERPQTDVGRILQGKVPGVNINGVSGISGTGTN
                     INIRGYTSISGNTQPLFVVDGVPYNSNTNAVSTGGGSATQNFTTGGQSTPSRFIDIDP
                     NNIENVSVLKGLAATVLYGDQGRNGVILITTKTGNKKFKAPSISLSTSLFGNQVAALP
                     EYQNLYGGGFQQVGGPAFFSNWGAGFHQLKETPHPYSLFSSAELRAAFPEFQGKNVAY
                     TGYKNNVSDFFRTGLVTNNSLVVNGGTDKFNYSASLGYANEEGYIENNDLNKLNIGLG
                     TNVAISDKISFSTSLQFSSFDQKTPPISGGQANNALDYPSIFANLFFTNRSNDLTGWP
                     FESPVDNRSVYYRAGNDIPNPYWIRKYERVTDQVNRLFSSSIINYKITPRLDLSYKVG
                     LDTYGELQELIVPKGDPLPSINTGLYRTANIDNTIWDHTVALTYQKPFSKSFGYTVKV
                     GGNMRNDFFRQDAIISTQQLAFNLNRHSNFLAVAANDFTIEQTRMGIFGNAIVDYKEW
                     VYLDLAGRYDWTSTVEKENRTIFYPSASVSFIPTSLFKGLESTTLGYLKLRFGVGQSA
                     GFPNPYNTRNVLAQNARAFTDASGTLHTTHSVANNLGNPNLKPELHSELEFGVEARML
                     RNRVSIDLTVYNRTTKDLITSALLDPSTGFTNTTINIGKLRNKGIELGLSGSPVKVGN
                     FEWETFINYSRNQPLIEELSNSLTSIQIAGFGSALGNYAEVGQPFNVIKGTKWRTNEQ
                     GQRLIDAGGNFLVTATPEVMGDPNPKFLTSFGNTLVYKNLALSAMLEYRHGGVIYSSM
                     AKATLGRGTSRDTEFDRDQTFILPGVKASDGTPNDVQITASDVYFNNVFFFGDEGSMY
                     DGTQLKLREVSLSYTVPAKLLGKTIKGASLTLSGNNLWNKAFNFPKYMNFDADMAGLG
                     VGNGIGFDMLVSPTSKRFGGSLKLTF"
     gene            26322..27977
                     /locus_tag="PKM27_06565"
     CDS             26322..27977
                     /locus_tag="PKM27_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNR06963.1"
                     /translation="MKNYIKIFLLSFGFLAASSCSLDLLDNPNAVTTSSTNLNFLLNN
                     IQIGYRNAFNGFSDIGLRLTRMLNQGAVIYDNAVTPGGVNGVWENTYAVTLADVNTLL
                     PLAEAQEFYFHTGIARVIRSHLLTMLVDHFGDVPYSKALDPNEFNPSVDKGADVYNAA
                     LADLDKALEDFGKSAKGTVNDFFYRGDKAKWIRLANTLKLRILLNRRLVDANAAGGIN
                     TLIAAKNLIENTSQSFYFKYGTNLANPDARHPRYQGQYTVTGGGDYQSNYYMRQFVDA
                     KGFADPRLRYYFYRQVLEDTKNANELRCITNQAPRHYPDGMVFCVAGSNSGGNNGYWG
                     RDHLNNEGIPPDGVKRTAWGLYPAGGSFDNDAGRAVSLGAGAGGAGIQPIMMASFVDF
                     MLAEAALVLKTTGDPKALLESGIKKSMDDVRNISMATSQGSVISAFESSKGYVYNDEV
                     TKYINEVKSRYDAATNDGDRLNIILTEYWLAAWGNGIEPYNFYRRTGKPAGLQPALDP
                     NPGEFVYSCYYPIAYVTRNSNAVQKQNVTTKVFWDTNPVGFVK"
     gene            28006..28560
                     /locus_tag="PKM27_06570"
     CDS             28006..28560
                     /locus_tag="PKM27_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06964.1"
                     /translation="MKKMLILSLGFVLFGSCEIDDLNKIDLLNLDNGAYMRTISPTAA
                     NMAFLKTELGTASLTFTVEAVDPDRGAQFGSYDLAVEFRDRTASNGNNSVALKNLKSI
                     APTGFTTDPTSGYLRGTFTISSAEIMTALGVTADKVSVGDSFDVFSTLVTKDGRKFSD
                     KNTDPDILGGAFYNSPFYYRLNIK"
     gene            28759..28956
                     /gene="rpsU"
                     /locus_tag="PKM27_06575"
     CDS             28759..28956
                     /gene="rpsU"
                     /locus_tag="PKM27_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNR06965.1"
                     /translation="MLIIDVKDSESIDKALKKYKKKYEKSQMLKQLRARKHFTKKSVE
                     RRNQVLKAAYRQQILSKPAEI"
     gene            29080..29979
                     /locus_tag="PKM27_06580"
     CDS             29080..29979
                     /locus_tag="PKM27_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNR06966.1"
                     /translation="MNGLRSFLNYLEFEKRYSPHTLEAYQNDLLQLQDFLSHTYQLED
                     LKEVQALHLRAWLVALHEQGLSNRSINRKISTLKSYFQFLRKRHGLDRNPVRKLVSPK
                     TEKRLPLVVRTEQMERLMVLEQTGDPDFHTFRDALVTEILYGCGLRRSELIQLRTDDI
                     DAGRNLLRVEGKGKKERRLPLGPALVDKLNQYLRLRRDYLSEKGLPDPTYLLITDAGE
                     PLYPKWVYNRVKAQLGLVTSISRRSPHILRHSFATHLSDAGADLNAVKTLLGHASLAA
                     TQVYMHNSVEKLKEIYRQAHPKS"
     gene            30032..30331
                     /gene="raiA"
                     /locus_tag="PKM27_06585"
     CDS             30032..30331
                     /gene="raiA"
                     /locus_tag="PKM27_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNR06967.1"
                     /translation="MKIMTESIHFTADQKLLHLIERKLQKLERLYERIIDIKVVLKLE
                     NHQTIKDKIVEILIHVPGGNLFARESSRNFETAIELALASLKKQTMRFKGKIQSN"
     gene            30553..30625
                     /locus_tag="PKM27_06590"
     tRNA            30553..30625
                     /locus_tag="PKM27_06590"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30586..30588,aa:Thr,seq:tgt)
     gene            30665..30750
                     /locus_tag="PKM27_06595"
     tRNA            30665..30750
                     /locus_tag="PKM27_06595"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30700..30702,aa:Tyr,seq:gta)
     gene            30814..30886
                     /locus_tag="PKM27_06600"
     tRNA            30814..30886
                     /locus_tag="PKM27_06600"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30847..30849,aa:Gly,seq:tcc)
     gene            30982..31053
                     /locus_tag="PKM27_06605"
     tRNA            30982..31053
                     /locus_tag="PKM27_06605"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31014..31016,aa:Thr,seq:ggt)
     gene            31118..32314
                     /gene="tuf"
                     /locus_tag="PKM27_06610"
     CDS             31118..32314
                     /gene="tuf"
                     /locus_tag="PKM27_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNR06968.1"
                     /translation="MAKETFQRSKPHLNVGTIGHVDHGKTTLTAAITSILAESGLAEK
                     RSYDSIDAAPEEKERGITINTAHVEYQTQNRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLAKQVGVPKIVVFMNKVDLVDDQEMLDLVEMEVRELLDQ
                     YGFDGAHASVIKGSALKALEGDPTYKQRVLDLMAAVDKDIPEPVRLVDQPFLMPVEDV
                     FSITGRGTVATGRIEKGIIKVGESVEIVGMMKDGEKPIVSTITGVEMFRKLLDRGEAG
                     DNAGLLLRGVEKDQIRRGMVICAPGSVKPHKKFKAEIYVLSKEEGGRHTPFFNGYRPQ
                     FYFRTTDVTGDVKLPEGVEMVMPGDNVTIEANLLNSIAMEKNLRFAIREGGRTVGAGQ
                     VTEILD"
     gene            32398..32470
                     /locus_tag="PKM27_06615"
     tRNA            32398..32470
                     /locus_tag="PKM27_06615"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32431..32433,aa:Trp,seq:cca)
     gene            32485..32673
                     /gene="secE"
                     /locus_tag="PKM27_06620"
     CDS             32485..32673
                     /gene="secE"
                     /locus_tag="PKM27_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNR06969.1"
                     /translation="MDKISLYLKESYEELKNKVTWPSWANLVDSAKVVLVTTVIITAI
                     VFVMDLLSNSILNVVYGL"
     gene            32682..33233
                     /gene="nusG"
                     /locus_tag="PKM27_06625"
     CDS             32682..33233
                     /gene="nusG"
                     /locus_tag="PKM27_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNR06970.1"
                     /translation="MADQEKKLYSLRVISGKERKIKERIELEIQRQGWNTFISQVIVP
                     SEKVYKIRNGKKVVLERNILPGYILLEADPSKLIPEVVQVIANIPNVIHFLGKENPIP
                     MSPGEANRLLGKVDESQEAGESMIEPFLPGETVKIIDGPFNDFVGDIKEINEEKKKLK
                     VIVKIFGRGTEVELNFMQVEKTS"
     gene            33346..33786
                     /gene="rplK"
                     /locus_tag="PKM27_06630"
     CDS             33346..33786
                     /gene="rplK"
                     /locus_tag="PKM27_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNR06971.1"
                     /translation="MAKEIETFIKLQVKGGQANPAPPVGPALGSKGVNIMEFCKRFNA
                     QTQDKQGKILPVVLTVFKDKSFEFIIKTAPAAIQLMEATKIKSGSKEPNRNKVADVTW
                     DQIKTIAEDKMPDLNAFTLESAMKMVAGTARSMGITVSGKAPWN"
     gene            33959..34651
                     /gene="rplA"
                     /locus_tag="PKM27_06635"
     CDS             33959..34651
                     /gene="rplA"
                     /locus_tag="PKM27_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNR06972.1"
                     /translation="MAKETKKRKVANAKVDRRKYLALKDAMALVKEVNTAKFDASVDI
                     HIRLGIDPRKSDQAIRGTVTLPHGTGKTKRVLVLCTPDKEAEAKAAGADHVGLDDYIQ
                     KISEGWMDIDVIIATPSVMAKIGKIGRILGPRGLMPNPKTGTVTQDVGAAITEVKGGK
                     IAFRVDKQAIIHSTVGRVSFTPEKLTENANELLTTVVRLKPTSAKGTYIKSISVASTM
                     SPGIWLDLKSIH"
     gene            34676..35203
                     /gene="rplJ"
                     /locus_tag="PKM27_06640"
     CDS             34676..35203
                     /gene="rplJ"
                     /locus_tag="PKM27_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNR06973.1"
                     /translation="MNKTEKGTTIEALKDKFDASTFFYLADASSMTVAQITKFRRLCY
                     EKGIEVKVVKNTLARKAMEALPESRGFKDLFDALHGPTAVLFSDQGNLPARILKEFRG
                     AGDKPVLKAAYIDSAVFKGDDQIEVLIAIKSKNELIGELIALLQSPARNVLGALQSGG
                     HKLAGLVKALEDRAN"
     gene            35266..35646
                     /gene="rplL"
                     /locus_tag="PKM27_06645"
     CDS             35266..35646
                     /gene="rplL"
                     /locus_tag="PKM27_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNR06974.1"
                     /translation="MADLKALADQLVSLTVKEVNELAAILKQEHGIEPAAAAVAVAAP
                     AGGGAAAAAVAEQTEFDVILKSGGAEKLKVVKEVKTLLNLGLKEAKDLVDGAPATLKE
                     KATKEEANTLKTALEAVGAEVEVK"
     gene            35793..39602
                     /gene="rpoB"
                     /locus_tag="PKM27_06650"
     CDS             35793..39602
                     /gene="rpoB"
                     /locus_tag="PKM27_06650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNR06975.1"
                     /translation="MAIKKADSKRINFGKIKHGTESPDLLEIQLKSFQEFFQLETTPE
                     NRSNEGLYKVFQENFPITDARNIFLLEFLDYFVDPPRYTIDECMQRGLTYSVPLKAKL
                     RLSCNDEEHVDFETIVQDVFLGNIPYMTPKGTFVINGAERVIVSQLHRSPGVFFNQTF
                     HPNGTKIYSARVIPFKGAWMEFSTDINTVMYAYIDRKKKFPVTTLLRAIGFDSDKDIL
                     DLFGLAEEVKVSPKHLQQNIGRKLAARVLKKWTEDFVDEDTGEVVTIERNEIILERDI
                     ILDEENIQLILEAEAETIILQKEEVEDDYTIIYNTLQKDPSSSELEAVQHIYRQLRGT
                     DPPDEETARGIIDKLFFSDKRYNLGEVGRYKINKKLEQEIDLGTMILTNEDIINIVKY
                     LVLLINQKAELDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNEVFTP
                     IDLINARTLSSVVSSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRERAGFEV
                     RDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKVNKMGFLETPYRKVKNGMVDTRTHA
                     EFLSAELEDNKKIAQANSGLDEKGAFLTDRIKCRLHGDFPVLMPEEVEYIDIAPNQIV
                     GVSASLIPFLENDDANRALMGSNMQRQAVPLINPASPIVGTGLEGKVAMDSRMLINAE
                     GDGTVEYVDANQIIIKYDRSEEDQLVSFEDNLKTYHLTKFQRTNQATCINLKPIVKKG
                     DRVHAGSILCDGYATDRGELALGQNLKVAFMPWKGYNFEDAIVISERVVQQDVFSSIH
                     IESFDLDVRETKLGDEELTNDIPNVSEEATKDLDENGIIRIGAWVNEGDILIGKITPK
                     GESDPTPEEKLLRAIFGDKAGDVKDASLKAPPSIEGVVIDKQLFAKAKKDKVQKGQEK
                     DVLDKLDQKHEVAVNQLKTILIDKLMRLLKGEATDGIRTIYGEQLVAKGAKFSPNMLK
                     KIDYASVDYTNWTSDKKINHTITRLLHNFSIKYNEEVGRYKREKFNVSIGDELPAGVQ
                     KLAKVYIAKRRKLKVGDKMAGRHGNKGIVSRIVRMEDMPFLEDGSPVDIVLNPLGVPS
                     RMNLGQIFETVLGWAGEKMGVKFATPIFDGATLGEIESYIEKANLPQYGQTYLYDGET
                     GDRFHQQATVGVIYMLKLSHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEV
                     WALEAYGASHILQELLTVKSDDIIGRAKAYEAIVKGENMPEPNIPESFNVLINELQGL
                     ALEVKFE"
     gene            39640..43920
                     /gene="rpoC"
                     /locus_tag="PKM27_06655"
     CDS             39640..43920
                     /gene="rpoC"
                     /locus_tag="PKM27_06655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNR06976.1"
                     /translation="MPFKKKSSKHPKYFDSITISLSSPDDILERSYGEILKPETINYR
                     SYKPERDGLFCERIFGPVKDYECYCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHI
                     KLVVPVVHIWFFKSLPNKIGYLLGLSSKKLESIIYYERYVVIQPGIKAKDGVNTLDLL
                     LEEEYLDILEGLPKENQFLDDTHPDKFVAKMGATAVKDLLERVILDDVSFDLRHKSAT
                     ESSQQRKSEALKRLRVVEAFRDGQKSQINKPEWTVIQYLPVIPPELRPLVPLDGGRFA
                     SSDLNDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKAE
                     GGRALKSLSDILKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLRLHECGLPKNMAAELF
                     KPFIIRKLIERGIVKTVKSAKKLVDRKDPVIWEILENILKGHPVMLNRAPTLHRLSIQ
                     AFQPRLVEGKAIQLHPLVCGAFNADFDGDQMAVHVPLSQPSILEAQLLMLSSHNMLNP
                     QDGSPITLPSQDMVLGLYYLTKEKRSTDKEKVRGEGSKFYSPEEVIIAFNEGKLDLHA
                     MIKMRAKVDQGDGKLIYKVIDTTVGRVLFNQAVPERVPFINELATKKGLKRIISEVIT
                     RTDFSETADFLDSIKDMGFYWAFKGGLSFNLGDLVTPSIKDITLKQAQTEVDEIWDNY
                     NMGLITNNERYNQIIDRWTFADNRITDSLMKELSNHKQGFNSVYMMLHSGARGSKQQI
                     KQLCGLRGLMAKPRKSGDTGAAIIENPILSNFLDGLTVLEYFISTHGARKGLADTALK
                     TADAGYLTRRLVDVSQDVVITEVDCGTLRGIETSALKDNEKIIESLASRIQGRFNLHT
                     IFDPVTDEVIMGPGEYIDDKTAEKIEAAGIEAVTIRSVLTCEARRGVCTKCYGKNLAS
                     GRIAEQGDAVGIIAAQSIGEPGTQLTLRTFHVGGIASISKTESELVAKFDGVIEFDNV
                     TYTQVKEEDHKINIILSRSGEIRVVDPKTKKQLSASHVPYGASLKVKEGQQVKKGDLI
                     CEWDPFNAVIVSEFSGIAQFSEIEEGVTYRMERDDQTGYSEKVIIDAKNKKKVPVIRI
                     ANKNGEDLKQYTLPVGAYISINDGDSVYAGQKIVKIPRKLGKIQDITGGLPRVTELFE
                     ARNPSNPATVAEIDGIVSFGKIKRGNREVMIEDEKTGQKSKYLIGLSKHLLVQEGDFV
                     RSGTPITDGTIAARDILHIKGPYAVQSHLVNGVQEVYRSQGININDKHIEVIVRQMMR
                     RVQIEDPGDTHFLESEAVDKHEFNEHNDWIFDKKFVTDSGDSTKLKPGALVSLRQIRE
                     ENSILKRNDKKLVEYRDALPATSSPLLLGITKSSLGTESWISAASFQETTKVLSQAAI
                     AASKDILNGLKENVIVGKRIPAGTGLRQFETLLVTAEDGFELDSVVQE"
     gene            complement(43981..44601)
                     /locus_tag="PKM27_06660"
     CDS             complement(43981..44601)
                     /locus_tag="PKM27_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNR06977.1"
                     /translation="MAKKQKYYVVWEGHSTGIFEDWKKCEAQVKNYPNARYKSFESLE
                     EARQAFFGGPSAKKPVFARARRKNYDHLVQDSIVVDAACEGNPGILEYQGVDPHSGAK
                     LFHRGPFQEGTVNIGEFLAIVHALAWLKQKGKHTTAVYSDSVTGLSWVRNRRIRTNLV
                     RSGRNEELFVLVDRALEWLAKNTYQNKLLKWDTETWGENPADFGRK"
     gene            44627..45418
                     /locus_tag="PKM27_06665"
     CDS             44627..45418
                     /locus_tag="PKM27_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNR06978.1"
                     /translation="MMLVDTHAHLYASEFDGDRLEMIHRAIQAGVGRIYLPNVDADTI
                     PALLSTCALSDILYPMMGLHPCQVTAGFTETLAEIKEELYRNSRLYYAVGEMGIDLHW
                     DKTTRPYQEEAFRVQCGWACELDLPVVIHSREATEAVLELLEPMDPRPARGVFHCFSG
                     DRHQVERIQALGNYYFGIGGVITYKNSGLAALVKNIPLDRIVLETDAPYLAPTPFRGK
                     RNEPAFLVNIAESLGRALGLPVDEIEKITTGNALALFERSGKVEG"
     gene            45523..45610
                     /locus_tag="PKM27_06670"
     tRNA            45523..45610
                     /locus_tag="PKM27_06670"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45557..45559,aa:Ser,seq:gga)
     gene            45770..>46189
                     /locus_tag="PKM27_06675"
     CDS             45770..>46189
                     /locus_tag="PKM27_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06979.1"
                     /translation="MRKIALLFTLVLVAFLAGGLELMAQEPAAAAAAPSGGFQMLKEK
                     FIEGGWQWMVPILFVLILGLAFCIERVITLNVATINTDKLLSKIDDRLQSNDIEGAIE
                     VCKVTPGPTASTLLEGLRHAEKGPDAVEKAISAFGGMQ"
BASE COUNT        12043 a        11887 c        11597 g        10662 t
ORIGIN      
        1 tagtcagtag tcagtagtca gtagtcagta gtcagtagtc aatagtcaat agtcagtagt
       61 cagttggcaa gtccctgtaa tttccatttg gtactcaaaa ttcatccttc agggcaaggg
      121 gccatcagcc tgcagcaatt cgcaaataat tttcagtggg aagctgtcca tcttgccact
      181 cccggattat tgagcaatcc accatcatga tagaaagcca tccgccttgt acttatcttc
      241 ctttaacctt taaccattaa cctttaacct taccttgctg aggtggatgc tttcactcct
      301 gctaacccca aatgggggat ttccatattt ataaaaaaat aagccgggtt ttttcatcac
      361 atcaacaaag ggtgaaagca gcggtaaata ttttattttc gttaagactt gtgaaaagat
      421 attaaaaaac cggaaccgca tttatttttg aggggttcaa gaatacctgc tgaacatccg
      481 gactccctga atcagactct tctaaataat tacattgaag tcaatggctt ttggtttcag
      541 gggcgcgtga tttcaatctt tttaaaaacc ttactctacc gggccttata aggcattttt
      601 aattgtctgt gaatgccaac tgcattcatc aggccctggg ttttatttcc tgttaactct
      661 taaatgcatt gatatgaaag tgtatcttat gtggatcctg tgtattggcc tgggctttac
      721 ggctcaagcc caacgcagga tcagcggtat catttcggat cagaacggag atcccctgat
      781 cggtgtaaac atcctggttc agggcaccaa caacggaacg gtttctgatg agaatggagc
      841 gtacagcatt gaggtccggc cggaaaaccg ggtcttggta ttcagttaca ccggctttac
      901 ctcccgggaa attgaaatcg gaacggccag taccctggac gtcaccctca ccgaaggggt
      961 gctcctggat gaaatggtag tcaccgccct ggggattacc cgggagaaaa aagaaatcgg
     1021 ttatgccatc gaggaaatca aaggggagga gatccagcgc gttggggaaa cagaccccct
     1081 gagggccatg cagggcaaaa ttgcaggcgt caacatcaac gcctccagtt cccagccggg
     1141 cagcgccacg cggttgacca tccgcggcct caaatcattc ttcggtaaca atcaggccct
     1201 ctatgtggtg gacgggatcc cgtttgatgc caccaccaat ggggtttcct ttcagctgac
     1261 cggcggaggc gcctatgcca acaggctggc cgacctggac ccaaataaca tcgaaagcgt
     1321 cagcgtattg aaaggcgcta cggccgccgc cctttatggc tcaagagctt ccaggggggt
     1381 agtggtgatc accaccaaaa ccggaactat taaaaagggg gaaaacagga tgaaggttga
     1441 attgacctct ggccttaact ttgaaaaaat cgcttccctt cccgattttc aaaacaagta
     1501 cggaaccggc accggttttc tctatcagca agccaacggg tcctggggag ccccatttaa
     1561 agggaccgtc ccttatccca ccattgattc catcaaccat tggtatctgg gcgccggtta
     1621 tgcggcgagt ttcaaccggt tggtgcaatc cggagacccg agagtcaccc agaaagttcc
     1681 ttaccaggct tatcccaata atgtaaaaga cgtcttcgag accggcaggc ttatggacaa
     1741 ttcgatcacc atcagcggag gcaatcaaaa caccaatatg gccctgactt tttcaagggt
     1801 cgatcacgcg ggcatcttcc ccaacagcaa ttttgataaa acctctcttt cccttggtgc
     1861 aaacagcaat tttgaatcgg gcctgaggat gggcgccaac ttatcttatg taaattcaat
     1921 ccaggaaggc ctccaaggcg gagcgatcgg gtccgtcagt acttcttcct ttatggccag
     1981 gaccctttac cagggcagaa actgggatct ccagggccag ccctatgagg atcccttgac
     2041 ccgggagagc ctgtttatgg tcgccagggg ccaggccgac aatccctatt ggtctgccag
     2101 gtataacgga tttgagtcca atgtgaaccg tttcgtggtc aatgtgaatg cggggtatac
     2161 cttatctaaa tggctggatg tggattaccg ggcaggattg aaccagtata accagggtga
     2221 tctcgaatgg ttcaggccag gctcccgggg agctggggga ctcggcagcg taaccgacct
     2281 gaataccaat ttcagagaaa tagaatcgac cttaatcttt acagccaagc accggtttaa
     2341 tgacagttgg gccatcgaag caagggccgg ccacaacctc aatgaaagga ccagcaaggc
     2401 ccagggtatt tccggcaccc agatgctcga tttcaatatc cttgatgtag acaataccgt
     2461 caaccaggtt tcagccggcg gcaacttctc caaacaaagg ctgtttggtc tttttggtga
     2521 gttcactacc gattacaaac aatatctttt tctcacgcta tcgggacgga atgactggtc
     2581 atccaccctg cctgtcaaaa acaacaatta tttctatccg tcggccagcg tagcctttat
     2641 tttttcagag gctttcaata tgatcaacaa cccgatcctc aatttcggta agatcaggat
     2701 gaattggtct aaagtgggaa gcccggcagc tccctattcc ctgaaaccca tatacctgct
     2761 caacctgggc gagcccgaca acctcactgc gggtatccgg gatattgatt ttccttttgc
     2821 aggcatagcc agttccacct tgtccaatac cttgtttgac cccgacctca aaccggagtt
     2881 taccaccgat cttgaagccg gtttggacct cgaattcctg aagggcagga tagagctcaa
     2941 tgtctctgtt ttcaagagta cttccgtgga ccagatctcc cagttggcct tgccgaccgt
     3001 atcgggttac gaccagttgc ttaccaattt cggggcactg caaaccaaag gatcggaagt
     3061 atcgctcagg ctgaacccca tatccaagaa aaattttaac tggaacatca tcaccaattt
     3121 tacacggtat aaaaccatcg tgaccgaact ccgggaaggt acggaagaaa tggtcatccg
     3181 ccccctgttc gccggcggga ttaccccggt tttgagagtg ggtcagcctt atggcatttt
     3241 aaaagggacg gtaagcgccc gggacgatga gggcaattta ttgatcgata aagccaatgg
     3301 acagatcatc aacggcatcg atgatgccat cgtgggagat cccaacccgg actttaccgt
     3361 aggcattgta aattcaataa gctggaaagg attatccctc ggcgccgttt tcgattggag
     3421 acagggtggg gatctttatt ctgagaccgt gaacgctttg ctgggacgcg gggttttgaa
     3481 cttccaggca gaccgagaaa aaaatgccgt gatccccggc gtctatgggg atccggtgac
     3541 cctgaaaccc atacttgatg aagccggacg cagcatccgg aaccagacga tggtggaaac
     3601 caatgacctc tggtttggca gtacttttgg caccaacagc caggatgagt ggtccgtatt
     3661 tgacgctacg gtataccggc tgagggaatt gtccctgagc tacagccttc cttccagcct
     3721 gtttaaaaaa ggaatccggg gagttactct ttcactgatc ggaagaaacc tttggtataa
     3781 ggcgccgcat ttccccgaag gcaccaacct ggatccggaa accagcacct tcggcgactc
     3841 caattaccag ggatttgaat tccagaacct gccgtcgatc aaacggtttg gcgccagcat
     3901 caatctcaag ttttaaccat taaaatcaaa gtcatgaaat tttacagatt gattttttta
     3961 atatgcgggt tcagtctgat ctccatctcc tcctgtgacg attacctgga tatcaacgac
     4021 gatcccaata atcccacgga cgccagagta aacaacctga tgcccttcag ccaggccgtc
     4081 gttttcggtt cactgggtat tggtacggcg ggcatttcag agttggtttc ggtgtacagc
     4141 caccataccg tgcaacgggg caatcacgat gattataaga tcaccgccaa tgaatttgcc
     4201 ctcacccagg cctgggaaaa gatgtatgcc gtggccctgc ctgacctcga catcatcatt
     4261 caaaaagcaa cggaggccaa caacttcgcc tatgtgggca tggccaagtt gctgaaggtg
     4321 cagatattca gttcggtcgt ggacctctgg ggggacatgc cttacagcga agccgccctg
     4381 ggggccgaag cgccctttcc caaatacgac gacgataagg aaatctaccg cgatctgttt
     4441 cgcctgggat ttgaagccct ggctgacctg gataaaccca gtacgctgaa acccaccacc
     4501 gatgacctgg tgtacaaggg aaatatggac aaatggaaaa agtatgggag gtccctcttg
     4561 ttgaatatgt acaacaaagc ccggctgacg gacctttacg acgccgccaa agtggctgaa
     4621 ttactcaaat taagccatat ctccgggacc agtgaagatt ttgaactggc ttatacgacc
     4681 tccatcaccc ctgaaaacag gaaccctggt tttgtcaggg aatacaccct cggcaatccc
     4741 acctattaca tcagccccta tttctacctg atgatgaaag gggaagccgc ctgtgaaaac
     4801 accttgctcg aagggattgc ggacccaagg atcccttatt tcttttataa tcagcttggg
     4861 cccaacgagc aggccgaaaa cccggtttct taccaggacg gatctttcct ctccatctgg
     4921 tttggttctt acgacaagga tcccaatgaa ggtttcgacc agggcaaatc gcaaaccctg
     4981 cttggcttgt ggccggtcgg gggagcctat gacaatggca gcggggccaa tggggacctc
     5041 aattcagggc tgaagggagc cggcgcccag cgtctgctta ctgcctcgga tgtaaacttt
     5101 atccgtgcgg aactggcctt gaccaaaaat acgggcgaag atgccaaagc tttgctgaac
     5161 acgggtatcc gttcgtcttt tgccaaggtc aatgccgtgg ccagactggc cggagcaccg
     5221 gtgatcccgg atgcggccat caccacttat gtggataagg tcctggcctt gtttgatgcg
     5281 gcggacaatg cccgcaaact ggaattgatc cttacgcaaa aatggatcca ttctttcggc
     5341 aacgggctgg agtcttataa tgatatccgc agaaccgggt tccccaaaat ttgcgatccg
     5401 gcgaaagatc caaatgtaca ttcctcacag tcccggcctt atccggtcac ccttccgtac
     5461 aatggggatg acctgcagac caatcccaac gcaaaacagc acaatcagta tacggacaag
     5521 gtattctggg atgtggatta accggatggt acgatttaac gatcatttat aaaaaatcta
     5581 aaaatgaaaa aaattcttat ttatataccg gccttactcc ttctggtaag gtgtacggat
     5641 gagagcaaga tccgcatccc ggagatcgct aatggggtaa acctgcgtat tgtcgtcgat
     5701 ccggcgcata ccgggatctt taccgataaa attgccacgg atttcctggc tttcgacctg
     5761 tactcggaaa acaaagacct ggcctccgta caaatttttg cggccaccgg gggcgtgaga
     5821 aagctcgcca aatccctgac ccagtccgat tttgaccagg gacagggaaa ggtccggatg
     5881 gaattgaaag cgtcggattt tgccaccctg tttggggagc ctgggtatgg ggatggttcc
     5941 aaagtggcaa actttacctt caatccgcag gtcacgctca acgacggccg ggtatatccc
     6001 tcctacgtaa aactgagtgc gacggattcg gtcctgaacc ttgccccatc catcaacgga
     6061 gctgcggctt cctcctttac cctgcagttt cagtccttta tcacctgccc ggcggtggat
     6121 atttcaggag attataaagt agaggatgcc accggcagtt ctactgacgg atgttgtccc
     6181 actgaaacca ccgtgaaggg catcgtggtc aagattacca aacttacggc gtcctccttc
     6241 aaactcagcg atttcagcgg ggggctttat tttgaatggt atgacgtcta tgggattaca
     6301 aaaccggaag actccccggg tgaaattgct ttcagttgca atgaagtctt tttccagaat
     6361 accaaggaac cctttgggga aaatgtatcc ggaagcggcg actacgaccc cgccacaaag
     6421 accctggtgt accattgggt gaatgggtat ggggatgaag ggacggtgaa gttggtgaaa
     6481 cagtaaccag gggccagtgg gtggtcaatg gtctcaccgc tcgcttctca ctgctcgtat
     6541 ctcgtatctc gtatctcgct actcgaattt cccgctccgc tctactacca tgcggggact
     6601 tagagtaagt tttttaaagc agctggacgt ataaagatat gacggctgct ttttttattt
     6661 ggggtgaggg gtgaggattg aggattgatc gaatccatct tcaatcctca aacctcaaac
     6721 ctcaatcctc attattactg actattcaca ttaatcttcc agtgcccctt tgattccaat
     6781 tcgaattcca ctctgaccgg caggacttcg ctgactcctt tttgggaagt ataattcaac
     6841 ctgactaaaa gggggaaact gacgttttcg atgatgatcc ggtttttacc ctggacataa
     6901 tgattgccgg tggatgcaaa gatattgatc ccgttgatca tggaccgggt accgaggaat
     6961 atttcgacgc ggtcctggat ctcgttgggt ttgtcaaact gggtcgacag ggtattcagc
     7021 gactggatga tcttgtattt tttttccagg atctcttcga taaactcatc atctttccac
     7081 aggctcagtt ttccggattg ggtggtactg gtttccttat ccatggtatg gagggtgcct
     7141 tctccatgac ggagcccgtt tttgaattca ccgatataat aggagccgtc cgaatagaca
     7201 tatttcccct tgccgtgagg gaccccttct ttaaaatgtc cctggtaggt atccgtaccc
     7261 tgggctgtgc cctccccatg agccaggcgg tttttgcatt ccccctggta ggtgccctgt
     7321 aattttgccg aaaggacccg gcaattggcg gtttgttgct gccccgatag ggggatggtc
     7381 aggagcatca gccccagtac accgatttta gtaaatttta acattttaga aattctttaa
     7441 caaaagaacg aattttaaga tcagtttgtc gaaaaataac agttttttga ttgaaattga
     7501 ctcaaagtga cagcgctttc aaactaaagt acgtttgacc tgaagttaag aaattaacca
     7561 tttcttaatt tattttttat tttcgcgcta aattttaaat ctaaatgatg aaaaaactta
     7621 ctctaacctt tgcaacgttt ttgtttgcag tggccattgg cttggcccag cgaacaatta
     7681 ccggcacggt caaggaggtc tccggagagc ccttgatcgg tgctaacgtc tatgtaaaag
     7741 gtactactac gggtaccact accgacatcg acggaaccta ttctctggaa gtcccgtccg
     7801 atgcatccat tttggttttt tcctatatcg gttacaccga ccaggaagtg accgttggta
     7861 atcagtccac cattgatgtg accctcgctg aagggatcac gctccaggaa accgtggtga
     7921 ccgccttggg gatcagccgt tcccagaagt cattaaccta ttccgcccaa caggtggatg
     7981 gagaccaact ccggcagatt ccagccactt cggtcaacaa tgccctgagt gggaaagtag
     8041 ccgggatcca ggtccggagc caatcgacgg tagctctcgg tcgcgatgct gcgatccgca
     8101 tccgcggcgc cggttccctc aatgacaaac aaccattata tgtagtagat ggaactccgg
     8161 tggccagcgc ccaggatatc tctgtggacg atattgcggc catgaccgtg ctcaaaggcc
     8221 ccagcgccac ggccctctat ggccagcgtg gggatgccgg ggtcatcgtg attcaaacca
     8281 agaaggccaa caataaacag gccctgggta ttgaaatcaa ttccggtacc ttcttcgacc
     8341 aggtctatgt cctgccccgt tatcaaaact cctatgcagg agggggcgca gccgacctga
     8401 ttgaattcaa ctggaaggaa ggcatgcccg atgaatggaa aatttttgaa ggcaaatacc
     8461 accatgacta ttccgatgat gcttcctggg gacccaggat ggtcggacag gaatacattc
     8521 cctggtatgc ctggtatgtc ggtactccgg ccttcggcca gaccgctaaa ctggttccgc
     8581 aacccaacaa tatccgggac ttctaccaga cgggggtaac cctcaacaac aacgtgagcc
     8641 tgagtaaagg gggagaaggg tataatgtcc gcctttccta taccaatcaa aacgtcaagg
     8701 gtttattgcc ctcttcctct gcaaataaga ataccatcaa taccagcgcc agcttcgacc
     8761 tgggtaaata tctgactgca agcaccaata tcaacgtatc caactggatc gtcaacgggg
     8821 tgtttgacga cggatatgcc aaccagtctt cgggctcttt caaccagtgg ttccaccgcc
     8881 acctggacct gaataagatc aaagaataca agggtatgaa atctccggaa ggcatcctgt
     8941 cttcctggaa ccacaacaat cccggaaatt acctgaccag cccgctctcc ttctatggtg
     9001 gcaactactg gtacaatttt tatgactttt ttgaaaacat cgaccagtct tccagacgga
     9061 cccgtttgtt cggggacgtc agcctgacct tcaaaccctt caaggacttg accgtccagg
     9121 gatgggtgag aaggaaccag atcgaggtgg tcaatgaagg ttttggacgg gcaatcctgg
     9181 agaacagcgc cacccagacc ggtttcaaat cttattattt ctacaaccag ggttattatg
     9241 aaccgagcga tggttcacca ggtaaagaag ataactacga ggccctggct tcttatcaca
     9301 aacgtttcgg agatctttcc ctggacgtga aagccggtgg aaatatccgg aaggtacagt
     9361 tctcccaaat atcaggatct accgtggacg gcctctcggt tcctgatctc tttaccctgg
     9421 cgaattccag gacgactccg atcactttcg gcagcggccg ttccttcaaa cgggtcaaca
     9481 gtttatatgg aaccggtacc ctgggctaca aagacttcct ctatgtcgat tttgccgccc
     9541 gtaacgactg gagttccgca ttgcccaaag atgccaactc ctatttctat ccttccgtgg
     9601 gagtaagttt cctcttcagc gaattgttgg ggattcatga cctttccttc ggtaaattga
     9661 gaggaagctg ggcccaggtg ggttctgacc tgggtgccta ccagcttgcc ctcaattatg
     9721 gggtaaattc caaccagtgg aacggggctt tcctcatggg taccccggat gccctggtgg
     9781 acccgaacat caaaccctcc ctttcatccg cttatgaagg agggcttgac ctgaaattct
     9841 tcagcaaccg tctcggcctt tccggtactt attacaagga gaaaaagatc gatgagatcc
     9901 tcaatgtacc ggtatccgca accagcggtt ttgcatctaa aaacatcaat gccggccggg
     9961 tggaccgcag cggggtggaa ctcacccttg aagtcacccc ggtttcaaac cgtaacgtaa
    10021 actggaacat gaccttcaac tgggcccgca accggaccaa gatcatcgaa ctggctgaag
    10081 gcatcaactc gatcatccgc gaaaccggta ccttcgggac cagttcaggc gcccgcctgg
    10141 tgcaccaggt cggtgaactc tggggccagg tacgcggcgg aggtatcaaa cgcaatgacg
    10201 caggagttcc tgttctggac gcttccggcc agttcatcaa cgtaccggat acttatttcg
    10261 gatctgtgct tcctgacttt accggtggta tcctcaatga gatcagcgtc ggcaatttgt
    10321 tcgccaattt taatatcgac ttctccaaag gcggggtgtt ctattctctc tcagaccatt
    10381 ggggcaaatt ctccggtttg ttcgaagaaa cggctgccct gaacgacaag ggtaacccaa
    10441 gccgcgaccc tgtggctgtc ggcggcggtg tgcatgtggt cggcgtagat gccgaaggca
    10501 aacctgtcga cacttacgta gatgcccaaa cctattggca tcagttccgc aacaagagta
    10561 ttgctgaaga caacattcat ccgctggatt ttgtaaaact ccgcgaggtg aacatcggat
    10621 acaaagtcaa taccaataaa ctcggaatcc ccgcggtgaa aaacctggtg gtttccttta
    10681 ttgcccgcaa tccctggctg atttcctcca agaccaagga ttttgatcct tcagagattt
    10741 ctcaatccta tggagaaaac ggacagttcc ccggaacccg ttccttcggc tttaacgtaa
    10801 aggttggatt ctaaaaaaat aaaacatgaa aaattcattt aaatcaatat taatcgttgc
    10861 agcagcgctg ctgttcagca cctgttcgct atccgatttc ggagacctga accgaaatcc
    10921 taatgcgacc accgtgcccg tcacctccgc cctcctgacc aatgctttgg ccgggttggg
    10981 tggctggacc cagggaatga acgaagcctt gtattgccag tatttttctg aaacgcaata
    11041 caccgatgcc tcgctctatt cactgatcca ggtcaatttc accggcaatt attccggggc
    11101 gatgtatgac ctgcagaaca tcatcgacaa caataccaat ccggatacca aagatgaggt
    11161 gctggccaat ggttccaatg ccaaccagat cgccgtggcc cggatcctcc tggtctatca
    11221 ttttatgcat cagacggacc gcttcggcga tgtgccttac agcgaagccc tgaaagggga
    11281 tgcccaaccc gcttacgata cccagttgga tatctacaaa gggttctaca aggaactgaa
    11341 agaggccatt gcccagtttg acaatggact tgccgcccag ggggatattc tatttgatgg
    11401 ggataaaaac aaatggaaaa aatttgccaa ctctctgaga ttgatcctcg ccatgaggct
    11461 ttccaaagcc gatgcaaacc tgggtaaggc tgaatttgca gcggccttct cagaccctgc
    11521 aggttacctt tcttccgcat cggaagatgt ggtcgttcat tatcccgggg ctgcctttaa
    11581 aaatccctgg tttaccctct atgatggccg taaggattat tcgatcagcg atgtgatggt
    11641 ggagcgcctg aagtcctgga atgacccgcg cctgccggta ttgggcaatc ccaatgcaca
    11701 gggcgcagtc aaaggggttc cttatggatt gacgcgtgac gccgcgattg catttaccaa
    11761 tgccaatgcc gactggtctc agatcctggc tcccaaatgg cgtgcccaga attcacccct
    11821 ggtggtctta tccgcttccc aggtcctcct gaccattgcg gaagctattc acctgggctg
    11881 ggtaaccggt gacaaagccg cattctacgc caatggcatc aaagcttcct gggaaagatg
    11941 ggaagtttac aatgaacagc gtttcctggg ttatattcag cccaccggaa tcggtaccga
    12001 actcattgcc ctgaccggtg gccccagcga cctcgaaaaa atcaatttac agaaatgggt
    12061 ggccatctat ccggacggaa gaagcggctg gaccgaatgg agaaggacca acaccccggt
    12121 cctgacccca acccctgctg ccgtgagcaa ttccaaacaa atcccccgcc gattcactta
    12181 tccctccgtg gaatacaacc tcaaccggga aaacctggat gcagccctcg gacgcctggg
    12241 tggcccggac aaccaggacg gacgcgtctg gtgggataaa taaacctatt tatttaaatt
    12301 acaattaaca caaatcgtat gaaattaaaa tatataatgg gtcttctggt cctggcctcc
    12361 ttcacttcct gtatcgagga aggggaaaac gccatcgaag ggatcggtgg caattttgtc
    12421 aggattgcgg gaggaggaga tgaagtcaaa gccatcggct tcagtgcccg gcctggctcc
    12481 gccacccttc cattgtttac cgttcaacgg gacgccaatt ctgaagccaa cctcaatact
    12541 tcggctgaag tgaccctggc gcttgacccg gccatgatcg cagcttacaa cgatgccaat
    12601 ggtaccgagt tcgtggacat gccggccaat ctttataact tgaaatccct gaacctggcc
    12661 tttgcccccg gggagtttgc caagaaggtg accattaccg tggatccgtc cactttcgac
    12721 ctgagcaagg cttacgcgat agggatcaaa gtggcttccg tatcgaatgg atataccatt
    12781 gtggaatcgg ctaaaagcgg ccttttcaac atcctggtta aaaacgacta cgacggaatt
    12841 tataaggtga ccgggaccat gacggattat gtgtcttcag cattaaccgg ttatttcccc
    12901 atgaattacc acctggtgac ttcgggagcg gtgactgtag atggctacga tccggatgtc
    12961 tggcatgcgc ctttcgttcc tatttacagc ggtaccgccg tatccggtta tggttcattc
    13021 tgccctgttt tcacttttga tcctgcaact aataagatca tcagtgtcac caattattat
    13081 ggtcaaccgg cggggaatac ccgtgcggca gtaatcgacc cttcgggcat caataaattt
    13141 aatgcggacg atcacagcat cgatgtcaaa ttttttatga cacaaaccaa tgccgttgcg
    13201 gccccacccc atatccgggt ggctttcgac tggcatatgg aatatttagg ccccaggtaa
    13261 tccggaaggc cttttatgaa aaaagctcct ggaacactcc aggggctttt tttatttttt
    13321 tacagtgaga aaggggacgg ggatacggtg gtattcgtta tattgcagta ttcgtgtata
    13381 gtcactaaat cattttttaa gctatttata tgaaaagact ttgccaactt gggctttcag
    13441 cctttttatt tctatttgca tttaccggtc tgcatgccca gtcggttaaa ggccttgttc
    13501 gcgccaaagc cgacaatcag cctttgatcg gcgtgtctat cagtgtgaaa ggtaccaata
    13561 ccggcaccgt gaccgatatc gatggcgcct atgaaatcgc agccggtcca aacgatgtcc
    13621 tggtcttcac ttatatcggt tttgctacag cggaagtggc cgtcaacggc caggctgtcg
    13681 tagacgtcat tctcagcgaa gatgtgaaaa ccattgaaga agtgatcgtc acgggatatg
    13741 gcacccagat caaacgcgac ctcaccggca atattgcgaa gatcaaagcc agcgagatcc
    13801 gcgatgtgcc cgtaaattcc gtggaggcgg tgctccaggg caaagccgcc ggtgtacaaa
    13861 tcaatgcagg cacgggaaaa ctgggtcagg cccagcagat ccgcattcgt ggcaattcct
    13921 cggtttccgc ttccaatgag cctttgttcg tagtggacgg cattcccatt accactgccg
    13981 atctttccaa caacggcggg ggctccacca attccatcgt ggacatcaac ccccaggata
    14041 tcgaatccat cgaaatttta aaggacgcct ccgcggccgc catctatggt tcaagggctt
    14101 cgaacggggt ggtcctgatc accaccaaaa aaggaaggga gggtcgcacc aacgtgaatg
    14161 taggaatgca gtatggtaac agcaaaccaa cccgtaaagt cgactttttc aattccgccc
    14221 agtttataga ctattaccgc aaggcagccg ccaattccga ccggattgaa agcatcgatc
    14281 cttccgatcc cgattcctac accacttata tggaaggatt cttcgaaggg gtgagcctgg
    14341 gcacctatgg tacccctaac caggcggata cccgctggga tgatctcgcc ttccaggatg
    14401 cgccccagac ccagatcgac ctgagcatcg atggcggaaa tgccaagacc cgcttctttg
    14461 tttccggcca gttcctcgac cagaaaggga tcatggtaga caataaactc aagagaatga
    14521 ccggaaggat caacctcgac caccaggtca gcgatatctt caacctcgga ttcaatctcg
    14581 gcctgagccg taccataaac gagcgtctgg acggggacaa tgccttttcc aatccgttgc
    14641 agcttgtagc cctcaccccg ctatcccccc aatttgaccc gaccacaggt ttgctgatcg
    14701 gttctcctcc cggtgatatc ggccaacccc tgtactataa tggcctcctc aatgtggacg
    14761 gcgccctatt caataccaat gtccaccgca acctgagcaa tgtctacggt gccctcagaa
    14821 tcatacccgg actggtgttc cggtctgaac tcggtattga tttcctcaat cagcaggaag
    14881 aatcttacaa cagcagcctc agccagagga ataccggttt cccgcttggg acgggctcca
    14941 attattttac ccgcgtggaa aacctgaata cgaacaacta ttttaattat ctcaaggaat
    15001 tcggatccca caagatcgac ttcacgctgg gcatgtccta ccagcaatcg gaacaggatt
    15061 ttaattccgt gaccgggacc gattacccgt cggattctta caaaaaaatt gcaagcgctg
    15121 caaaaatcac ggcagggact tccacggaga cgaatttcag attcgtgtct tatttcgcca
    15181 gggccaatta caagctttcc gataaatacc tgatcgggct cagcgcccgc gtggatggct
    15241 cttcgcggtt cggttcagac agccgatatg gatttttccc ggctgtttcc gcgggctgga
    15301 tcctctcgga agaagacttt ctgagcgaca gcaggctcgt cagtttcctg aagttaaggg
    15361 ccagttatgg acgcaccgga aacgccgaga tcggcaattt cccgcaactg ggtttgttcc
    15421 agggagatgc cggatatggg aaccagccag gtcagcggcc cgcccagttg ggtaatccca
    15481 atctcaaatg ggagaccacc gaccagatgg atatcgggct tgattttgga ttgtttaatg
    15541 accgcctgaa tggagaaatc gactattaca ataagcagac ggatggcttg ttgctgaatg
    15601 taaatgtacc tgccagcact ggatttagca cccagaccaa aaacgtggga aaactggaaa
    15661 actcaggtat tgaatttgca cttaacggca cggtggtcaa cggtcgggac ttcaaatgga
    15721 acatcggggt caattatgcc aggaataaaa atctgatcaa tgacctccag ggtcagatca
    15781 tcgaaggcgg cctttccaat atgagccggg ccatggaagg ccagcccctg agtacctttt
    15841 ttacggtaga atatgcaggg gtggatcctg cgaatggcga cgcgctttgg tataaaaatt
    15901 caaaaaatac agatggcacg atcgatcgcg gcactaccaa ccggtacaac caggctcagc
    15961 gggttgtgat aggtaacgga cttccaaagt gggttggggg cctgactacc agcatcagtt
    16021 ttaaaggact ggattttaat atgttatgga ataccgtaca aggcaacctg gtcaacttct
    16081 atggagccgg ccggttcagt tctgccaatg gccgttttga agacaaccag acagtagatc
    16141 agctgaattc ctggacccct caaaatacca acacgaatat tccggaagcc cgtctgtttt
    16201 acaataacgg ggcccaggcg tccagccgct ttatccagga cggctcattt gtccgcctga
    16261 gaaatgcaac cctcgggtac agtcttcccg cggtttggct taaaaatgtg ggcatccaga
    16321 gcgccagggt ttatgtcacc ggattgaatc ttttgaccat taccgattat accggctggg
    16381 acccggaagt caattcagat gatttcgtta cgaatgtcgc gcaaggttat gatttttatt
    16441 cacccccgct cccgcggacc attgtcggcg gtttaaacat taaattttaa ttcaatcaaa
    16501 tcctcaggaa tcatgaaaaa gtcaatctat atatttctga ttttcatctt cgcggcctgc
    16561 gacaaggaac tggatgtcaa gcccacccaa tccattgatg aaacgaatgc cctggcgacc
    16621 gcccaggatg taaaggtcac cctgaccggg gcctatgacg ggcttgcgga tggagatgtt
    16681 tacggtgggg gatttcaatt taccagtgaa ttggtggcag atgaccggga ggtgcggttc
    16741 atagggactt tcgccggaca ggatgaaatg tggcgcaaag ccatgacgac gggaaatgcc
    16801 caggttctgg ctacctggag agacgcttat gtggccatca accgggccaa caatgtactg
    16861 tcggccctgg acaaactgga tgccgccgat aaaagccagg ttgagggaga ggcccgactg
    16921 atccggggtg ccacctatct tgggttgatt aacctttttg ccaaagcctg gggcgatgga
    16981 gatcaaaaca ataatcccgg agtacccctg gtgaccactc ccacccgggt agtcaccgat
    17041 gaagacaatc gtccaagggc tactgtggca gccgtctatg cccagatcct ggaagacttt
    17101 accaaggcgg aaaccctgat tcccgaatcg gcggaccaaa atggattcgg cacccgcaat
    17161 gccgcgaccg cccttctggc caggacttac ctgatccagg gtaattttgc cgctgcgcgg
    17221 gatgcggccc acaaagtcat tgcctcaggt tcgcatgcct tggccgaatc ttatgtggat
    17281 gttttttacg ataagtccgg cagttttgac ggagaaagca tattcaaagt gattatcacc
    17341 gaacaggatg gggtgaatag tatgaatact tattttgccc cggcgacttt tgcgggaaga
    17401 ggggatatcg ttattcaaac caaacatact gacctttacg gtcccaatga cccaaggggg
    17461 caatttacag tgaccacatc ccgtggggta tttaccggaa aacatctgga tcagtttgga
    17521 gatatcagca tcatccggtt agctgaaatg tacctgatca gggccgagac caatttcagg
    17581 ctgggtacgg tggtaggtgc cagccccctg gaggacctca acgccataag ggacagggcc
    17641 ggagccggac tgttgaccct ggaccagatc aacctggacc ggatcctgct ggatcgaaaa
    17701 ctggagctga tgtttgaagg ccagttgctg caggatgtca ggcggcttaa aaagaatgta
    17761 ggaacgctcc cgttttccga tcctaagatg gtgctgccca ttccccagag ggagatcgat
    17821 accaataaga gcctggtcca aaacagcggc tattgatctg gtgaggagga attctgctgc
    17881 aggtctgagg cggagtaccc ggttttacag ttcaggcccc cattgtgcga gaatcggcac
    17941 cctgaaagtt tcggggtggc tcacggtttg atcattcaga aaacaacgag tttacggttc
    18001 ctgtggacct taaactttta aggatatcgg ttggctggat aaggccaatc gtttttatta
    18061 atttttttta aaacaagcat atgaaaagac ttattctact attcattttg ttgattaccg
    18121 tttcgacggc cttcacgcag cggaccatca aaggtatggt cacggatgaa gccggcgaag
    18181 cgctcatcgg ggccaatgtg gtggccaaag gatcctccat cggcaccgtt acggatgtgg
    18241 atggcgccta ttccatcatc gtccctgaaa atgtgacgga actgaccttt tcttacaccg
    18301 ggttcaatcc caaggatgta tctattggca ctttatccga aatcaatgtc atcctggaag
    18361 aaggggtgag catccaggag gtggtggtca cctcctttgg tattaaaaag gataaaagca
    18421 acctcgggta caacgtatca caactgaaca gcgaggaact cacgatggcg catacgacca
    18481 acatcaccaa tgcgatttca gcaaaggtgc ccggagtccg cacttctggc gccgggggct
    18541 ctttcgcgag ttccagcatt atcatcaggg gatttacctc cttcaccggc tccaatcaac
    18601 cactgtttgt ggttgatggg attgccattg acaatacggg agggggctcc gccttacaga
    18661 atggggtgac caattcgagt cgcgccattg acctcaacca ggacgatatc gaaagcatca
    18721 gcgtcctgaa aggggccgca gccacttccc tctacggctc aaggggagcc aacggcatca
    18781 tcctgatcac caccaagagc ggcaaggtca ggacgaagca aaacatctct tataatttga
    18841 cttatgccaa ccaggaaatc aaccgcttgc cggactatca gaatgaatac gcggccggca
    18901 atgcaggcgc atacaatcct aacggccttt cttcctgggg cccaaggatt gacggccgcc
    18961 aggtcttgct tcccaccgtt taccgtggcc tgaacggccc cggggatacc tcgatgaccc
    19021 tgcaggctta tcccaataac gtgaaggacc ttttccggtc cgcccccaat atgcagcaca
    19081 acctcagttt ccagggcggg aaggagaatt acagctaccg cttgtccgcc ggatacctgg
    19141 atgataagtg gatcatggac cagaaccgcc tgaagcgtta caatatcggc gtgaatgcca
    19201 ataacagcat tacaaaaaaa ttgaccgcag gggtttccgc caattatatc cggaacacct
    19261 cggtcaggac accacaggga aatcagttgg cgaatcccct cttccgtgcc tggtttaccc
    19321 cccgcagttg ggatcttact ggtcgtcctt ttgagcaccc ggtgaccctc aacaatctcg
    19381 attggggaag cggggcgcac gataatccct attggaccat taagaataac ctttacaacg
    19441 acgaggtcga ccggatcttt ggaaatttta atttacggta tgacatcagc aaaatgcttt
    19501 ccgccagcgt aaaggtggga acggacaact tcacttattc ggccagcctg tacgatcaaa
    19561 tcgggtccac gggtggagcc ggtaccagcg cggggggccg cggagccatc agggatatcc
    19621 gcaccgtatc acgcatactc aatacgacca ccctcctcac cgctcaaaaa gatatcggcg
    19681 attttgacct gaccctcgta ttgggaaatg aaatcctgga tcagttccgc agcgatgccc
    19741 aggtcaccgg ctatggcgtg acggtacgga atttcagaaa cctcaatgcc aacacgacca
    19801 cttatttccc ggtctttaat aaatggaatt accgcttggt aggtaatttt gccaacttta
    19861 cggcgggtta taagaattgg ggttcgatcg atctgtccgc acggaatgac cagaactcca
    19921 tccttccgca atccaataac agctattatt attattcggc agccatcaag ctgaacatca
    19981 tgaagatgtt cgctttgaat tcgcagtttt tatccgattt tgccatcctg gccaacaccg
    20041 gtttgatcgg gaatgccaaa cctgaattcc ggtatgctac agacagttat tacggcggag
    20101 cgggcgccgc ggacggattc gggcccaata ttgctttccc tttcaatagc ttgtctggtt
    20161 ttaccttgca gaatggggcg ggcaatcccg atctgaaacc ggagtttacc cgttcgagtg
    20221 aaatcggcgt aaacctgggc ttttgggaag accgtatcgg gatcgaagtc agcgcttatc
    20281 agcaaaaatc aaccgatatc atcctgtcgg tgcccaattc atcggcagca gggatttcga
    20341 gcattatccg caatgccggg gtactgaaaa ccaaaggttt tgagggcatc ctgaatattt
    20401 caccgatccg gagcaaggat tttcaatgga atgcttcggt caactatact caattcaaat
    20461 ctacggtcga ccagctggct cccggagttc agaacatttt cctgggaggg ttcactaccc
    20521 ccaatatacg cctggtggtc ggagatgaat acgggcagat ctatggatcg gattataacc
    20581 ggaccaacga tccaaccggc aagttgtttg accccaggct gccgtacaat cccaacggaa
    20641 cggtggtcat cggggccaac gggcttcctt ctgcgacctc gggagttcaa aaaatcggga
    20701 accccaatcc aaaattcctg atgggcatca acaatgaact gaagtataaa ggattatccc
    20761 tgagcgtatt actcgatatc aaagagggag gcgaccagta ttcccgcaac ctggcggaca
    20821 ttcagcgcaa cggtgtgggg atcgaaacgg cagaattcga ccggttcaat tccgatggaa
    20881 cccttgccaa gccttatatc tttgaaggag ttaaagcaga cggaactccc aataatgttg
    20941 cggtcactgc cgagcaatat tggggcaatt ccgggaaatt cgtcgcagcc aagggattta
    21001 tttacaatac ttcctggttc cgtgtccggg aagcggcttt aagctatacc ttttcacggg
    21061 gcttgctgga ccggacaccg ttcggcagcc tcatcgtcgg ggtttttgga cgcaatcttt
    21121 tcttatcggc acccgattat ccgcacctgg atccggagca gaatgccctt ggggtgagca
    21181 atgcccaggg attggaattc aatgccctgc ctcaggcccg gtccctggga gttaacctgt
    21241 cggtgacctt ttgatgatca cctgttgaaa tcatattatt tatttaatta gaaagagaaa
    21301 tgaaaacaaa taaatatata aaactgagtc tcctccttgc ggtgatgctg ttttcaaccg
    21361 tgtcctgtaa ggattacctg gatatcaacc aggaccccaa tgcggtgctc aatgcgccca
    21421 tcgaacaagt gctgacctcc gccacggtcg ctttcgggtt ttgggcgggc agcgatatga
    21481 accgttatgg agccctgatc gcgcagcaat ggtccggtca aaccgccggg gcaacggtcc
    21541 agacccagga ctatgagaaa tacctgatta ccgggtccga tgtaaacaat gtctggaatc
    21601 tgatgtatgc caccatttta agcgaccttg aactggtcat tcagaaagcc ggcgaagaag
    21661 gcagtccgca ttatgcgggg gttgccaagg tattgaaggc ttatgtcttt gccaatatcg
    21721 ccgacacctg gggtaaggcc ccttttacag aggccttgaa atttaccgca aattcacaac
    21781 cgaattacga cgatgcttcg gtcatctatc ccgccctcat cgctatgctg accgaagcgg
    21841 tctcagacat gaatgccgct tcccagaaaa gcccggggac caattcgacg atctattcgg
    21901 gttcctggtc cgcttctcgg gccaaatgga ttgccgcggc caataccctc cggatgaggt
    21961 tgtggctcca taccaccaaa gtggaccggg caaaagcggt atcggaaatt gcagccatcg
    22021 ctgcgacggg accggtcatc aatgccgtag ccgacaattt tcaaatggct ttcgtcaacg
    22081 cgaccaatgc ccagaatccc attcagcaat ttgaaatttt ccgttcgaac tacctgtttg
    22141 caaatgccac cttggtggat atgatgaatg ccaaggaaga tcccaggcgc tggcgttatt
    22201 ttacgagttt ccccctgcac agcggcaatt tcaaaggggc caaaagcggc gatccctctt
    22261 cccagcaata ctcccgcacc catatatatt tgcgcgggga taccctcaat accgccacgg
    22321 cggcaagcaa cggttcgatt gctttcaatg catacaccta ttccggcact gctcccgtcc
    22381 gttttttgac cgccgccgaa tcctttttta tccgggctga agcggccgtc ctcagcggag
    22441 gcaacctggg ggggctgtct gccgattcct tgttccgcca gggtatccgg atgtccatga
    22501 gcgccgccgg ggtgtccacg gcgcaagccg aagcttatat cgcacaacaa ggcagtttac
    22561 cgggaactga agcggaaaat gtgaagaaga tcatcgagga gaaatatgta ggaaatttcg
    22621 gggtaatcaa tgagccctgg gcggactggc ggagaacggg atatccgtct caaatagtca
    22681 aagtgccgaa cgccgtggtc accagcagcc ctgacgttcc ccgttccttt ttttaccctc
    22741 agtctgaaat cgatctgaac ccgaatgctc ctccacaaaa agtcaatatg gtggaaaggg
    22801 tgttttggga taaataaggt ttaggatcaa agtccactga aaaaagccgg aagcctggta
    22861 agggtttccg gctttttatt ttaaaacaaa cccatccact tttatttctg cccaaatcag
    22921 gggatccttt gccaaaaggg gggattttag cttggaaaat tggcaatttc ccggcctaaa
    22981 attgtttttt ctaaatttta aggaaatttt aatcgagata ttgattttta ttaccttcaa
    23041 tccacaataa aggttaccga aacgttaaag gattggtaaa acctttaact gggatactca
    23101 ctcattaaat ctatttaaat cagggttata tgaaaagact ctttctattc gtatgcctcc
    23161 tgggcagtgc aatttcagct tttgggcaga ggaccatcag cgggaaggtc accgactcgg
    23221 gcggcgaagc attaattggc gccaacgtgg tggccaaggg taccgagatc ggtacagtta
    23281 ctgacattga cggatcttat tccatcaatg tccctggtga agtgacaata cttacattta
    23341 cgtacacggg ttttacaccc aaggatgtgg tgattggaaa tgcctccgag ctcaacgtaa
    23401 tcatggaaga aggggtagcc atccaggaag tggtggtgac cgcgctgggt atccagcgtg
    23461 agaaacgggc cctgggttat tccgtgacta cggtagataa aaaactgatc gaagagcggc
    23521 ctcaaaccga cgtcggcagg atcctgcaag gcaaggtacc gggggtcaat atcaatggag
    23581 tttccggtat ttcggggacg ggtaccaaca tcaacatccg gggttatacc tcgatttccg
    23641 gcaacaccca gcctttattc gtggtggacg gggtacctta taattcaaat accaacgccg
    23701 tcagtacagg agggggttcg gcaacccaga attttaccac cggcggtcaa tccaccccaa
    23761 gccggtttat cgatattgac cctaacaata ttgaaaacgt atctgttctc aaagggcttg
    23821 ccgccacggt cttatatggt gaccagggcc gcaacggggt aatcctgatc accacgaaaa
    23881 caggtaataa gaaatttaag gcgccgtcca tttctttgtc tacctctctt tttggaaacc
    23941 aggtggccgc cttaccggaa tatcaaaacc tttatggcgg cggcttccag caggttgggg
    24001 gaccggcctt tttctccaac tggggcgcgg gtttccatca gttgaaggag actcctcatc
    24061 cttacagttt gttctcgagc gccgaattgc gggccgcttt tcctgaattc cagggtaaaa
    24121 atgtggccta taccggttac aagaacaatg tcagcgactt cttccgcacg ggcctggtaa
    24181 ccaacaattc gctcgtggtc aatggaggaa ccgataaatt caattacagc gcctccctgg
    24241 gctatgcgaa cgaagaagga tatattgaaa ataatgatct gaataaactg aatattggtt
    24301 tggggacaaa cgtagcgatc agtgataaaa taagcttttc cacctcgctc cagttttcaa
    24361 gtttcgatca gaaaacccct ccgatttcgg gaggccaggc caataatgcc ctggattatc
    24421 cgtccatctt tgccaatttg ttttttacaa accggagcaa tgacctgacc ggctggccct
    24481 ttgaaagtcc ggtggacaac cggagcgtat actaccgggc cgggaatgat atccccaacc
    24541 catattggat caggaaatat gaaagggtaa cggaccaggt aaaccgtctg ttcagttcaa
    24601 gcatcataaa ttataaaata accccaaggt tggacctttc gtataaagtg ggtctggata
    24661 cttacgggga actccaggaa ctgatcgtgc ctaaaggaga tccccttccc agcatcaaca
    24721 ccggccttta ccgcacggcc aatatcgaca acaccatctg ggaccatacc gtggccctga
    24781 cctatcaaaa accattctcc aagtctttcg gttacaccgt caaagtggga ggaaacatgc
    24841 ggaacgattt tttccgccag gacgccatca tcagcaccca acagcttgca ttcaatctta
    24901 accgtcacag caatttcctg gctgtggcag ccaacgattt taccatcgaa caaacccgta
    24961 tgggaatttt cgggaacgcc atcgtggatt ataaggaatg ggtttacctg gacctcgccg
    25021 gccgttacga ctggacttcc accgttgaaa aggaaaaccg gactattttt tatccctcgg
    25081 ccagcgtttc cttcattccc acctccctgt tcaaaggatt ggaatctacc accctgggat
    25141 acctcaaact ccgttttggg gtagggcagt ccgcgggctt ccccaacccc tacaacaccc
    25201 ggaatgtgct ggcgcaaaac gcgagggcct tcacggatgc aagcgggacc ttgcacacca
    25261 cccactccgt ggccaacaac ctggggaacc ctaatctgaa acctgaattg cacagtgaac
    25321 tggaattcgg tgtggaggcg cggatgctcc gcaaccgggt cagcattgac cttaccgttt
    25381 acaaccgcac caccaaagac ctcatcactt ccgccttgtt ggatccgtcg accgggttta
    25441 ccaataccac catcaacatt ggaaaattga ggaataaggg gatcgaactg ggattgagcg
    25501 ggtctccggt caaggtgggt aattttgaat gggaaacctt tataaactat tcccgcaacc
    25561 agcctctcat tgaagaattg agcaacagcc ttacctccat tcaaatagca ggttttggtt
    25621 ccgcactcgg taactatgcc gaggttgggc agcctttcaa tgtgatcaaa ggcaccaaat
    25681 ggcggaccaa tgagcagggc cagcgtttga tcgacgcagg aggtaacttc ctcgtcacag
    25741 caacccctga agtgatgggg gatcccaatc ccaaattcct gaccagtttt ggcaataccc
    25801 tggtttataa aaacctggcc ctgagcgcca tgctggaata ccggcacggt ggtgtgatct
    25861 acagttcgat ggccaaagcc acgctcggac ggggtacttc cagggacacg gaattcgacc
    25921 gggatcagac cttcatcctt ccgggggtaa aagccagcga cggtactccc aacgatgtgc
    25981 agattacggc ttcggacgtt tatttcaaca atgtattctt ctttggggat gagggatcga
    26041 tgtacgacgg gacccagctg aaactccgtg aagtttcgct ctcctatacc gttcctgcca
    26101 aattgctggg taaaaccatc aaaggagcaa gcctcacgct ttcggggaat aacctctgga
    26161 acaaagcttt caatttcccg aaatacatga atttcgacgc cgatatggcc ggattgggtg
    26221 tgggcaatgg aataggtttt gacatgttgg tcagtcccac ttccaaaagg tttgggggtt
    26281 cactcaaact gaccttttaa tcactccaaa ctgtaagtaa aatgaaaaac tatataaaaa
    26341 tctttttatt atccttcggg ttcctggccg ccagttcatg cagcctggat ttgttggaca
    26401 acccgaatgc ggtgaccacc agcagcacca acctcaattt tctgctgaac aacatccaga
    26461 tcggataccg caacgcattc aacggttttt ccgacatcgg attgcgtctg acccggatgc
    26521 tcaatcaggg tgcggtgatc tacgacaatg ccgtgactcc tggtggggtc aacggagtat
    26581 gggaaaacac ttatgcggtc accctggcgg atgtcaatac ccttttgccc ctcgccgaag
    26641 cccaggaatt ttatttccat accggaatcg cccgggtcat ccggtcgcat ctgctgacca
    26701 tgctggtgga ccatttcggg gatgtccctt attccaaggc cctggatcca aacgagttca
    26761 atccttcggt ggataaaggc gcggacgtat acaatgccgc gctcgcagac ctggataaag
    26821 ccctggaaga ttttggtaaa tccgcaaaag gcacggtcaa cgattttttc taccgtggcg
    26881 ataaagcaaa gtggatacgt ttggcaaata ccctgaaatt gaggatcctg ctcaaccgcc
    26941 ggctggtcga tgccaatgct gccgggggca taaacacgtt gatcgcagca aaaaacctca
    27001 ttgaaaatac ctcccaaagt ttttatttca agtatggcac caacctggcc aacccggatg
    27061 cgaggcatcc ccgttaccag ggacagtata cggtcaccgg aggaggggat taccaatcca
    27121 attattatat gcgtcagttt gtggatgcca aagggtttgc agatcccagg ttgcgctatt
    27181 atttctatcg ccaggttttg gaggatacaa aaaatgcgaa tgaactgagg tgcataacca
    27241 accaggcgcc gcggcattac ccggatggca tggtattctg tgtcgccggc agcaacagtg
    27301 gcggcaacaa tgggtactgg ggaagagacc acctgaacaa cgaagggatc ccgcccgatg
    27361 gagttaaacg cacagcctgg ggattatatc ctgcaggagg ttcgtttgac aacgatgctg
    27421 gtcgtgccgt atctctgggt gcaggtgccg gcggtgccgg tattcagcct attatgatgg
    27481 cctcctttgt tgattttatg ttggcggagg cagccctggt gctcaagact accggcgatc
    27541 ccaaagcctt gttggaatcc gggattaaga aatcgatgga tgatgtccgg aatatttcca
    27601 tggctacctc acagggttcg gtaatctctg catttgaatc cagcaagggt tatgtgtaca
    27661 atgatgaagt gaccaagtac atcaatgagg tcaaatcccg ttatgatgcc gcaaccaacg
    27721 atggagaccg gttgaatatc atccttacgg aatactggct ggcagcctgg ggaaatggga
    27781 tcgaacctta taatttctac agaaggaccg ggaagcctgc aggcctgcaa cctgctttgg
    27841 atccaaatcc cggggaattt gtttattcct gttattaccc gattgcctat gtcacgcgga
    27901 attccaatgc ggttcagaaa caaaatgtga ccaccaaagt attctgggac accaatcccg
    27961 tgggatttgt aaagtaattt cttaaatgaa aaatgagatt gaactatgaa aaaaatgttg
    28021 atattatcct taggttttgt tttgttcgga agctgcgaga tcgatgatct taataaaatc
    28081 gacctgctta acctggacaa tggggcctat atgcgtacca tttctccgac agcagccaat
    28141 atggctttct tgaaaacgga actgggtacg gccagcctga cttttaccgt ggaagccgtg
    28201 gatccggacc ggggagctca atttgggagt tatgacctcg ctgttgaatt ccgtgaccga
    28261 acggcctcca acggcaataa ttccgtcgcg ctcaaaaacc tgaaaagcat tgctcccacc
    28321 ggctttacga cggaccctac ctcgggttat ctcaggggga cttttaccat ttcttctgca
    28381 gagatcatga ctgccctggg tgtgaccgcc gacaaggtga gtgtgggcga ttcttttgac
    28441 gtcttttcca ccctggtgac taaagacggg cgtaaattca gcgataagaa taccgatcca
    28501 gatattctgg gaggcgcttt ttacaattcg cctttttatt accggttgaa catcaaatga
    28561 ccttcggctt aagccccggg aaaaccccgg ggcttttttt tgcccggcag cggccggctt
    28621 gaattttaat ggatcattta cgcaaagcga cggtcgcctt aggctcatca ggccggaaat
    28681 cttcttcaga taaactgtaa ttttttctta gttttttatg gatttctctt aactttgcat
    28741 cccaattcaa aacgatttat gttaatcatt gatgtaaaag acagcgagtc catcgacaaa
    28801 gcactgaaga agtacaaaaa gaagtacgaa aaatcccaga tgctcaagca gttgagggcg
    28861 cgtaaacatt tcactaagaa atctgtcgaa cgccgcaacc aggtgctgaa ggccgcatac
    28921 cgccagcaga tcctgagcaa gcctgccgaa atataagccg gtattcccac cgcacgcgcc
    28981 cgaagccccg ggcgcagtac gacgaacacc cttcattgcc tgccgccttc ttgattttaa
    29041 tggtacaact gtatatattg cagttataat agatttccat tgaacggatt aaggtcattt
    29101 ctgaactatc tggaatttga gaaaagatat tcgccgcata ccctggaggc ttatcaaaac
    29161 gacctcctcc aattgcagga ttttctcagc catacctacc agctcgaaga cctgaaagaa
    29221 gtgcaggccc tccacctgcg cgcctggctg gtcgccttgc atgaacaggg cttgtccaac
    29281 cggtccatca accggaaaat ctccaccctg aaatcctatt ttcaatttct ccggaaacgg
    29341 catggactgg accggaatcc cgtgcgaaaa ctggtgagcc ctaaaaccga aaaaagattg
    29401 cccctggtcg tgcgtaccga acaaatggag cgcctgatgg tcctggaaca aaccggcgat
    29461 ccggatttcc acacgttccg cgatgccctg gtgacggaaa tcctctatgg ttgcgggttg
    29521 cggcgcagcg aattgatcca gctcaggacc gatgacatcg atgccggccg gaatctttta
    29581 agggtggagg gcaagggtaa aaaagaaaga cggctgcccc tggggcccgc cctggtggat
    29641 aagttaaacc aatacctaag attgagaagg gattaccttt ctgaaaaggg attgcctgat
    29701 ccgacttatt tgctgatcac ggatgccggt gaaccgcttt atccaaaatg ggtgtacaac
    29761 cgcgtgaagg cccaattggg actggtcacc tccatatccc gaagaagtcc ccatattctc
    29821 cggcacagtt ttgccacgca tttgtccgat gccggggctg acctcaatgc cgtgaaaacc
    29881 ttgctgggcc atgcttccct ggcggctacc caggtgtaca tgcacaacag cgtggagaag
    29941 ttgaaagaga tctaccgaca agcccaccca aaatcctgaa ggtgggaaat aaagcctgaa
    30001 gcatttcgtt acaaattaaa aataccgaat tatgaaaatc atgacagaaa gcattcattt
    30061 tacggccgat cagaaactat tgcacctgat cgagaggaag ctccaaaaac tggagcgctt
    30121 gtacgaacgg atcattgaca tcaaggtggt gctgaaactg gaaaatcacc agaccatcaa
    30181 agacaagatt gttgaaattc tgattcatgt gccgggaggc aacctgtttg ccagggaaag
    30241 cagccggaat tttgagaccg ccatcgaact ggcgctggct tctttaaaaa aacaaaccat
    30301 gcgatttaag gggaagatcc agagcaattg atcccctgaa atcacttcaa aaatttttta
    30361 taaaaggctg ctccggcagc ctttttttat tacaggaccc tcgagacggg gcccctttaa
    30421 gtattgaaaa tatttttttc caaacaaaaa aatgttcgta aatttgtgcg cgcttttaaa
    30481 gaaagtggcc tatttatttg aaaatcagga gtgaagaaac acatacaagc agctgtctgt
    30541 gatgttaaat ccgccgatgt agctcagctg gtagagcagc tgacttgtaa tcagctggtc
    30601 gggggttcga gtccctccat cggctcccaa caaattggat gtgattttag gacggtatta
    30661 tattgggggc gcgccggagt gggagagccg ggccagactg taaatctggt gtcgctgact
    30721 gaatgggttc gaatccaatc gcccccacca attcataccc gttaagggtt tgagatcgtg
    30781 gtcgttgatt cgggatgctg aaaaatatga tcagcgggag tagctcagtt ggtagagcga
    30841 cagccttcca agctgtaggt cgcgggttcg aacctcgtct cccgctctgt gaacatcccg
    30901 cccacgggga cgggatgggg atttgtggag tagtcaaagg aatattggac gtgtccggaa
    30961 gtgacgctcc gatgctgcaa agccaatgta gctcagaggt agagcacttc cttggtaagg
    31021 aaggggtcga gggttcaatt cccttcgttg gctcaaattt gaattcagtt tgaaaatcat
    31081 tatatcattt aattttaaat aaaaacggtt cttaaccatg gcaaaagaaa catttcagag
    31141 gtcaaaacct cacttaaacg ttggtacgat cggtcacgtg gatcacggta aaaccaccct
    31201 gaccgcggca atcacctcaa tcctggctga atccggcctt gccgaaaaaa gatcttatga
    31261 ttccatcgat gcggctcctg aagaaaagga gcgcgggatc accatcaata ccgcgcacgt
    31321 ggaataccag acccaaaaca ggcactatgc ccacgtggac tgccccggcc acgccgacta
    31381 cgttaaaaat atggttacgg gggctgccca gatggacggc gccatcctcg tggtggcggc
    31441 taccgacggc cccatgcccc agacccgcga acacatcctc ctggccaaac aggtcggggt
    31501 gcccaaaatc gtggtcttta tgaataaagt ggacctcgtg gacgatcagg aaatgctcga
    31561 cctggtggaa atggaagtac gcgaattgct cgaccagtac ggcttcgacg gcgctcatgc
    31621 ttcggtgatc aaaggctccg ccctgaaggc actggaaggt gaccccacct ataaacaaag
    31681 ggttcttgac ctgatggctg ccgtggacaa agacattccg gaaccggtcc gcctggtaga
    31741 tcagcccttc ctcatgcccg tggaagacgt attctccatc accggccgcg gtaccgtagc
    31801 tacaggccgt atcgaaaaag ggatcatcaa agtgggtgaa tccgtggaaa tcgtcggtat
    31861 gatgaaagac ggtgaaaaac cgattgtttc caccattacc ggtgtggaaa tgttccgcaa
    31921 actcctcgat cgcggggaag ctggtgacaa tgcagggctc ttgcttcgtg gcgttgaaaa
    31981 agaccagatt cgcagaggga tggtcatctg tgcgcccggt tcggtaaaac cacataaaaa
    32041 attcaaagca gagatctatg tattgagcaa agaagaaggg ggacgtcata ccccgttctt
    32101 caacggctac cgtccccagt tttacttcag gacgacagac gtaaccggtg atgttaaatt
    32161 gccggaagga gtggaaatgg tcatgcccgg agacaacgtg accatcgaag ccaacctgct
    32221 caactccatc gcgatggaaa aaaatctgcg ctttgcgatc cgtgaaggcg gacgtacggt
    32281 aggcgccggt caggtaacgg aaattcttga ctaagcgcgg gccggaattt gatataatga
    32341 tattcgggaa gcatcttcca tccggaaggt gcttctttgt cataaaaaaa accagtaacg
    32401 ggcatagctc aattggtaga gcgacggtct ccaaaaccgt aggctgcggg ttcgattcct
    32461 gctgcccgtg caaccgaaaa atatatggac aaaatcagtt tatatcttaa ggaatcctac
    32521 gaagagctga aaaacaaggt gacctggcct tcctgggcca acctcgtcga cagcgccaag
    32581 gtggtcctgg tcactacggt gatcatcacg gccatcgtct ttgtcatgga cctgctttcc
    32641 aactccattc tgaatgtggt ctacggatta taatgaacac catggctgac caggaaaaaa
    32701 aactgtactc cctgcgcgtg atcagcggga aagaacgcaa gatcaaggag cggatcgaac
    32761 tcgagatcca gcgccagggc tggaatacct tcatcagcca ggtcatcgtg ccttctgaaa
    32821 aagtgtataa aatccggaac ggcaaaaaag tggtgctgga gaggaacatc ctgcccgggt
    32881 atattttgct cgaagcggat ccttccaaac tgatcccgga agtggtccag gtgattgcca
    32941 atatcccaaa cgtgatccac ttcctgggca aggaaaaccc gatacccatg agcccgggag
    33001 aagccaaccg cttgctcggc aaggtcgacg aatcccagga ggccggggag tccatgatcg
    33061 aacccttcct gcccggagaa accgtgaaga tcatcgatgg cccgttcaac gactttgtcg
    33121 gggacatcaa ggaaatcaat gaagaaaaga aaaaactgaa agtcattgta aaaatattcg
    33181 gaagaggtac agaggtggaa ctgaatttca tgcaggtgga aaaaacctct tgatgaatta
    33241 aaaaaaatga aaggaattaa ggaattaaat aattaaagaa taaggatgcc tccattctgc
    33301 ttccatggag cggccgattt ataaaaacag aaacgaaatt gtagtatggc aaaagaaatt
    33361 gaaaccttca tcaaattgca agtaaaaggg gggcaggcca atcccgctcc gccggtcgga
    33421 cccgccttag gttccaaagg ggtcaacatc atggaatttt gcaaaagatt caatgcgcag
    33481 acccaggaca aacaaggcaa aatattgccg gtggtcctca cggtttttaa ggacaaatcc
    33541 tttgaattta tcatcaaaac cgctcccgct gcgatccagc ttatggaagc cactaaaatc
    33601 aagtccggct cgaaagagcc taaccggaac aaagtggccg acgtgacctg ggaccagatc
    33661 aaaacgatcg ctgaggataa aatgcccgac ctgaacgcat ttaccctgga atcggcaatg
    33721 aaaatggtgg cgggtacggc gaggagcatg ggcattaccg taagcggtaa ggcgccctgg
    33781 aattaatttg aaattatttt ttttcaccga gggaggggag gactaaacgt ggtctcccgc
    33841 atgaaccttg aagaacgcaa aagagaaatt attctttgga tagtttcctt tggtggactt
    33901 caggtcgttg tgaggacccg ttctgtctgc cccgatcatg acctctaaat tctgcataat
    33961 ggcaaaagaa actaaaaaaa gaaaagtggc caacgccaaa gtggaccgga gaaaatacct
    34021 ggccctcaaa gacgcgatgg cattggtgaa agaagtcaac accgcaaaat ttgacgcctc
    34081 cgttgacatt cacatcaggc tgggtattga tcccaggaaa tccgaccagg cgatccgggg
    34141 taccgtaacc ctgccgcatg gaaccgggaa aaccaaacgg gtactggttt tgtgcactcc
    34201 cgacaaggaa gccgaagcca aggcggccgg cgcggaccat gtcggactgg atgattatat
    34261 tcagaaaatt tccgaaggct ggatggacat cgacgtgatc atcgctactc cttccgtcat
    34321 ggccaagatt ggaaaaatcg gccggatcct gggaccccgc ggactcatgc ccaatcccaa
    34381 aaccgggacc gtcacccagg acgtgggcgc cgccatcaca gaagtaaaag gcggtaaaat
    34441 cgcattccgg gtggacaaac aggcgatcat ccacagcact gtgggccggg tgtcctttac
    34501 ccctgaaaaa cttaccgaga atgcaaacga actgctgacg accgtggtca gactcaaacc
    34561 cacgtccgcc aagggaactt atatcaagtc gatatcggtg gccagtacca tgagcccggg
    34621 catctggctt gatcttaaat ccattcatta atcatcaagg taaacctgaa ttaaaatgaa
    34681 taaaacagaa aaagggacga ccatagaggc cctgaaagat aaattcgacg ccagcacctt
    34741 cttttacctg gccgatgctt catccatgac tgtagcccag atcaccaaat tccgccgcct
    34801 gtgttatgag aaaggcatcg aagtaaaagt ggtcaaaaat accctggccc gcaaagccat
    34861 ggaggcgctg cccgaatcca gggggttcaa agacttgttc gatgcactgc atggccccac
    34921 cgcggtcctg ttttcggacc agggcaattt gcccgcccgg attcttaagg aattcagggg
    34981 cgccggggat aagccggtcc tgaaagctgc ctatatcgac agcgctgttt ttaaagggga
    35041 cgaccagatc gaggtgctga tcgctatcaa gtccaagaat gaactgattg gtgaactgat
    35101 cgccctgctt cagtctcccg cccgcaatgt cctcggggcg ctgcaatccg gtggccacaa
    35161 actggccggc ctggttaaag cgctggaaga cagggccaat taatacggct tccaagtacg
    35221 catatatatt aaaatttaat caaaatcaaa aaaaagtaac ctatcatggc agatttaaaa
    35281 gcacttgctg atcaactggt aagcctgacc gtaaaggagg tcaatgagtt ggctgcaata
    35341 ttaaaacaag aacacggcat cgagccggcc gctgctgcag tagccgtcgc tgccccagcc
    35401 ggaggcggcg ccgccgccgc tgctgttgct gaacagaccg agtttgatgt gatcctgaaa
    35461 tcgggaggag ccgaaaaact caaggtggtg aaggaagtaa aaacgctcct gaacctgggc
    35521 ctcaaggaag ccaaagacct ggtggacggc gcacccgcta ccctgaagga aaaagcaacc
    35581 aaggaagaag ccaataccct gaaaactgcc ctcgaagcag tcggtgcaga agtggaagta
    35641 aaataaaatc ctatcggttt atttccaccg cgcatagata ttatcctgta gcggcctggc
    35701 taaatacctt gtattcggcc aggcctttac ggttttgtaa agacccgttg agaaaacctg
    35761 aattattaaa aatctaaatt cgcaagtggc ttatggcaat caagaaagca gattctaagc
    35821 gcatcaattt tggcaaaata aaacacggaa ccgaatcccc cgatttgctc gaaatccagc
    35881 tgaaatcctt ccaggaattt tttcaactgg aaaccacacc ggaaaaccgg agcaacgaag
    35941 gactctataa agtgttccag gagaatttcc ccatcacgga tgcgagaaat atatttttac
    36001 tcgagtttct ggattatttc gtggacccgc cgcggtatac catcgatgaa tgtatgcagc
    36061 ggggcctgac ttacagcgtc cccttaaagg caaaactgag gctgtcctgc aacgacgaag
    36121 aacacgtgga tttcgagacc atcgttcagg atgtattcct cggcaatatt ccctacatga
    36181 cccccaaagg cacctttgtc atcaacgggg cggaaagggt gatcgtaagc cagttgcacc
    36241 gctccccggg tgtgtttttc aaccagacct tccaccccaa cggcaccaaa atctattcgg
    36301 cccgggtcat ccccttcaaa ggcgcctgga tggaattttc cacggacatc aataccgtga
    36361 tgtacgccta catcgaccgc aagaagaaat tcccggtcac cactttgctg agggccatcg
    36421 gttttgactc cgacaaggac atactcgacc tttttggcct ggctgaagag gtgaaagtca
    36481 gccccaaaca cctccagcag aacatcgggc gcaaactggc cgcccgggtc cttaaaaaat
    36541 ggaccgaaga ctttgtggat gaggacaccg gcgaggtcgt caccatcgaa aggaacgaaa
    36601 tcatcctcga gcgcgacatc atcctcgacg aggaaaacat ccagttgatc ctggaagccg
    36661 aagccgaaac catcattctc cagaaagaag aagtggaaga tgattatacc atcatctaca
    36721 atacgcttca gaaggacccc tccagttccg aactggaagc ggtacagcat atctaccgcc
    36781 agctgagggg caccgatccg ccagatgaag agaccgcgcg cggcatcatc gacaaactct
    36841 ttttctccga caaacgctac aacctgggcg aagtcggccg ttataaaatc aataaaaaac
    36901 tggaacagga aatcgacctc ggtacgatga tcctgaccaa tgaagacatc atcaacatcg
    36961 tcaagtacct ggtccttctc atcaaccaga aagcggaact ggacgacatc gaccacctga
    37021 gcaaccgccg ggtacggacc gtaggcgaac aactctatgc ccagtttgga gtgggcctgg
    37081 ccaggatggc aaggaccatt cgggaacgca tgaatgtacg cgacaacgag gtcttcacgc
    37141 cgatcgacct gatcaacgcc cggacccttt cctcggtcgt ctcatcgttt ttcgggacca
    37201 gtcagttgtc ccagttcctg gaccagacca accccctttc ggagatcacc cacaagcggc
    37261 ggatatccgc actcgggcct ggtggacttt ccagggagcg ggccggcttc gaagtcaggg
    37321 acgttcatta ttcccactac ggccgcctct gtaccattga gaccccggaa gggcccaata
    37381 tcggtctgat ctccacgttg tgcgttcacg ccaaagtgaa taagatgggc ttcctggaaa
    37441 ccccttaccg caaggtcaaa aacggaatgg tcgacacccg gacccatgct gaatttttat
    37501 ccgcagaact ggaagacaat aaaaaaatcg cccaggccaa ttcaggactg gatgagaaag
    37561 gcgccttcct taccgaccgc atcaaatgcc gcttgcacgg ggacttcccc gtattgatgc
    37621 cggaagaagt ggaatatatt gacattgcgc ccaaccagat cgttggggtg tccgcatccc
    37681 tgatcccctt tcttgaaaac gacgacgcca acagggccct catgggatcc aacatgcaac
    37741 gccaggcggt acccctgatc aatccggcat ccccgatcgt cggcaccggc ctggaaggca
    37801 aggtggccat ggacagccgg atgctgatca atgccgaagg cgatgggacc gtggaatatg
    37861 tcgatgccaa ccagatcatc atcaaatacg accggtctga ggaagaccag ttggtctcct
    37921 tcgaagacaa tctcaaaact taccacctta ccaaattcca gcgcaccaac caggcgacct
    37981 gcatcaacct gaaacccatt gtcaaaaagg gcgaccgggt gcacgcggga agtatcctct
    38041 gcgatggtta cgctaccgat cgcggcgaac tcgccctggg acaaaacctc aaagtggctt
    38101 tcatgccctg gaaaggatac aattttgaag atgcgatcgt gatttccgaa agggtggtgc
    38161 agcaggacgt cttttcgtcc atccatattg aaagtttcga cctcgacgtc cgggagacca
    38221 aactggggga tgaagaactc accaacgaca tccccaacgt cagcgaggaa gccaccaagg
    38281 acctcgatga aaacgggatc atccgcatcg gcgcctgggt caacgaaggg gatatcctga
    38341 tcggtaaaat cacccccaaa ggggaatcgg atccgacccc cgaagaaaaa ctcctccggg
    38401 ccatattcgg agacaaggca ggcgatgtga aggacgcctc cctcaaagcg cctccatcca
    38461 tagaaggcgt ggtgatcgac aaacagctct tcgccaaagc caaaaaggac aaggttcaga
    38521 aaggccagga aaaagacgtc ctggacaagc tggaccagaa gcacgaagtg gctgtgaacc
    38581 agctcaaaac cattctcatc gacaaactga tgcgtttgct caagggggaa gccaccgatg
    38641 ggatccgcac catctacggc gaacaactgg tagccaaagg ggccaagttt tcgcccaaca
    38701 tgctcaagaa aatcgattat gcttccgtgg attataccaa ctggacgagc gataaaaaga
    38761 tcaaccatac cattacccgc ctgctgcaca acttcagtat caaatacaat gaagaagtcg
    38821 gaaggtacaa gcgtgaaaaa ttcaatgtga gcatcggcga cgaattgccg gccggtgtgc
    38881 agaaactggc taaagtctac attgccaagc gccggaaact gaaagtgggt gataaaatgg
    38941 ccggccgtca cggaaacaaa ggcattgtct cacggatcgt acggatggag gatatgccct
    39001 tcctggaaga cggctccccg gtggatatcg tgctgaatcc gctcggcgtg ccttccagga
    39061 tgaacctggg ccagatcttt gaaaccgtac tcggctgggc cggtgagaaa atgggggtca
    39121 agtttgccac tccgattttt gacggggcta ccctgggaga aatcgaatct tatatcgaga
    39181 aggccaacct gccccaatat ggccagactt atctgtacga cggggaaacg ggtgaccggt
    39241 tccaccagca agccaccgtc ggcgtgatct acatgctgaa attgtcccac atggtggacg
    39301 ataaaatgca cgccaggtcc atcgggcctt acagcctcat tacccagcaa cctctgggcg
    39361 ggaaagccca gtttggggga cagcgcttcg gggaaatgga agtctgggcg ctggaagctt
    39421 acggggcttc gcatatcctg caggaattgc tcaccgtgaa atcggacgat atcatcggcc
    39481 gggccaaagc ctacgaagcg atcgtcaaag gcgaaaacat gccggaaccc aatatccccg
    39541 agtccttcaa cgtattgatc aacgaattgc aaggcctcgc cctggaagta aaatttgaat
    39601 agtcaggaat tgtttaatca ttcttaaatc acttggttta tgccatttaa aaagaaaagc
    39661 agcaaacatc ccaaatattt tgacagcatc accatcagcc tttcctcacc ggatgatatc
    39721 ctggaaaggt cctatggaga gatcctgaaa ccggaaacca tcaactaccg ttcctataaa
    39781 ccggagcggg acgggctctt ctgcgaaagg attttcggcc ctgtcaagga ctacgagtgt
    39841 tactgcggta aatataaaag gatccggtac aaaggcatcg tctgcgaccg gtgcggagtg
    39901 gaagtcacgg agaaaaaagt gcgccgggaa cggatggggc atatcaaact ggtcgtgccc
    39961 gtggtccaca tctggttttt taaatccctt cccaataaga tcggatacct cctggggctt
    40021 tcctcgaaaa aactggagtc catcatctat tacgaaagat atgtggtgat ccagccgggg
    40081 atcaaggcga aagacggggt caataccctc gacctcctgc tggaggaaga atacctcgac
    40141 atcctggaag gtcttcccaa ggaaaaccag ttcctggacg atacccatcc ggacaagttt
    40201 gtcgccaaga tgggcgccac cgcggtgaaa gatctgcttg aaagggtaat cctcgatgat
    40261 gtttctttcg acctgaggca taaatcggcc accgaatctt cccaacagcg taaatccgaa
    40321 gccctcaaaa ggctccgggt cgtcgaggcg ttccgggatg gtcagaaatc ccagatcaat
    40381 aaaccggaat ggaccgtcat ccagtatttg cccgtcatcc cgcccgaact caggcccctg
    40441 gtacccctgg acggtggccg gtttgccagc tcggacctga acgacttgta ccggagggtc
    40501 atcatccgga acaaccgtct caaacggctg cttgaaatca aagcgccgga ggtcatcctc
    40561 cgcaatgaaa aaaggatgct gcaggaagcc gtggacagcc tctttgacaa cagccgcaaa
    40621 tccaatgcgg tgaaggccga agggggcagg gctttaaaat ccctgagcga tatcctcaaa
    40681 ggaaaacagg gccgcttccg gcaaaacctg ctcggtaagc gggtggatta ttccggccgt
    40741 tcggtcattg tcgtggggcc cacgctccgg cttcatgaat gcggtctgcc caaaaatatg
    40801 gcagccgaac ttttcaaacc cttcatcatc cgcaagctga tcgaaagggg aatcgtgaag
    40861 accgtgaaat cagccaaaaa actggtagac cggaaagatc cggtgatctg ggaaatactt
    40921 gaaaacatac tcaaagggca tcccgtaatg ctcaaccggg ctcccacgct tcacaggctt
    40981 tccattcagg ctttccagcc caggctggtc gaggggaagg cgattcagct gcatcccctg
    41041 gtttgcggcg cgttcaatgc ggactttgac ggggaccaga tggccgtgca tgtaccgctg
    41101 agccagccct ccatcctcga agcccagctt ctgatgttgt cttcgcacaa tatgctcaat
    41161 ccccaggacg gttcacccat cacccttcct tcccaggaca tggtattggg attatattac
    41221 ctgaccaagg aaaaacggtc caccgataag gaaaaggtga ggggagaggg ttccaaattc
    41281 tattcacccg aagaagtgat catcgcgttc aacgaaggaa aactggacct gcatgccatg
    41341 atcaaaatga gggccaaggt ggaccagggg gacggtaaac tgatttacaa agtgattgat
    41401 actaccgtag gccgggtgtt gtttaaccag gccgtgccgg aaagagtgcc cttcatcaac
    41461 gaactcgcca ccaaaaaagg gctgaaaagg atcatcagcg aagtgattac ccggaccgat
    41521 ttttcggaaa cggccgattt cctggattca atcaaggaca tgggattcta ttgggccttt
    41581 aaaggggggc tgtccttcaa cctgggcgat ctggtgaccc cttccatcaa ggacatcacc
    41641 ctcaaacagg cacagacgga agtcgatgag atctgggaca attacaatat gggtctgatc
    41701 accaacaatg aaaggtacaa tcagatcatc gaccgctgga cctttgccga caaccggatc
    41761 acggacagcc tgatgaaaga actgtccaac cacaaacagg gattcaactc ggtgtacatg
    41821 atgctccatt ccggcgcaag gggttccaaa cagcagatca aacagctgtg cggacttcgg
    41881 ggcctcatgg ccaaaccccg gaaatccggc gacaccgggg ctgccatcat tgaaaacccc
    41941 atcctatcca acttcctgga cggactgacc gtattggaat actttatttc cacgcacggt
    42001 gcccgcaaag gtcttgccga caccgcgctc aaaaccgcgg acgccggtta cctcacccgg
    42061 aggctggtgg acgtatccca ggatgtggtc atcaccgaag tggattgcgg taccctccgc
    42121 ggcattgaaa cgtccgccct taaagacaat gaaaaaatca tagagtccct ggcttcgagg
    42181 atccagggca ggtttaacct gcatacgatt ttcgacccgg tcaccgacga ggtcatcatg
    42241 ggcccggggg aatacatcga tgataaaacc gcggaaaaga ttgaagcagc ggggattgaa
    42301 gccgtaacca tccggtccgt actgacctgc gaagcccgca gaggggtctg caccaagtgt
    42361 tatggcaaaa acctggccag cggaaggatt gccgaacagg gcgacgcggt gggcatcatc
    42421 gcggcgcagt ccatcgggga accgggaacc cagcttaccc tgaggacctt ccacgtgggg
    42481 ggtatcgcct ccatctccaa aacggaatcc gaactggtgg ccaaatttga cggggtcatc
    42541 gaatttgaca acgttactta cacccaggtg aaggaagagg accataagat caatatcatt
    42601 ctgtcccgtt ccggggaaat cagggtcgtc gatcctaaaa ccaagaagca gctcagcgcc
    42661 agccacgtgc cttacggtgc ttccctgaag gtgaaggaag gccagcaggt gaaaaaaggc
    42721 gacctgatct gcgaatggga tccgttcaat gcggtcatcg tttccgaatt ttccgggatt
    42781 gcccagttca gcgaaattga agaaggggtt acctaccgga tggaaaggga cgatcagacc
    42841 ggctattctg aaaaagtaat catcgatgcc aagaacaaga aaaaagttcc ggtcatccgg
    42901 atcgccaaca aaaacgggga ggacttaaaa caatatacgc ttccggtggg cgcttatatc
    42961 tccatcaacg acggagattc ggtttatgca ggccagaaaa tcgtaaaaat tccgagaaaa
    43021 ctgggcaaaa tccaggatat cacggggggc cttccgcggg ttaccgaact atttgaagca
    43081 agaaacccct cgaacccggc caccgtggcg gagatcgacg gtatcgtgag ctttggaaaa
    43141 atcaaacgcg gaaaccggga ggtgatgatc gaggatgaga aaaccgggca gaaaagcaag
    43201 tacctcatcg gcctctccaa acaccttctt gtacaggagg gtgactttgt acggtccggt
    43261 acgcccatca ccgacgggac gatcgcggcc agggacatcc tgcatatcaa agggccttac
    43321 gccgtccagt cccacctggt caatggggtc caggaagttt accgctccca ggggatcaac
    43381 atcaacgaca agcacattga agtgattgtc cgccagatga tgcgccgggt gcagatcgaa
    43441 gatccgggcg atacgcattt cctcgaaagt gaagcggtgg acaaacacga attcaatgag
    43501 cataacgact ggatttttga taaaaagttt gtgaccgatt ccggcgacag caccaaactg
    43561 aaaccaggtg ccctggtcag tctaaggcag atccgggagg aaaacagcat cctgaaacgc
    43621 aatgacaaaa agctggtgga gtacagggat gccctgcccg ccacttcgag tcctttgctc
    43681 ctgggtatta ccaagagttc cctgggcact gaaagctgga tttcggcggc gtccttccag
    43741 gaaaccacca aagtcctgtc acaggctgcc atcgcggctt ccaaggatat tctcaacgga
    43801 ttgaaagaga atgtcatcgt cggtaagcgg ataccggccg gcacgggact gaggcaattt
    43861 gagaccctgc tggtgacggc agaagatgga tttgagctgg attccgtggt tcaggaatag
    43921 accgttaata acaagaggtt ttactataaa aggggaggcc gtgaaacggt ctcctttttt
    43981 ttattttctt ccgaaatcag ccggattttc cccccaggtt tcggtatccc atttaagcaa
    44041 tttgttttgg taggtatttt ttgccagcca ctccagggca cggtccacca atacaaacag
    44101 ttcttcgttt ctcccgcttc tgaccaggtt ggtccttatc cgccggtttc tgacccagct
    44161 gagtccggtc acggaatcgg aatagaccgc cgtggtatgt ttaccttttt gtttgagcca
    44221 ggccagggca tgtacgatgg cgaggaattc cccgatattc accgtgcctt cctggaaagg
    44281 gcctctgtgg aacagtttcg ctccgctgtg agggtccact ccctggtatt ccagtattcc
    44341 ggggttgcct tcacaggcgg catcgaccac aatgctgtcc tggaccaggt ggtcgtaatt
    44401 tttcctgcgg gcccttgcga aaaccggctt tttggcggag gggccgccaa aaaaggcctg
    44461 cctggcctct tccaggcttt caaaggattt gtagcgggcg tttgggtagt tttttacctg
    44521 ggcttcacat ttcttccagt cttcaaaaat cccggtggaa tggccttccc agaccacata
    44581 gtatttttgt ttcttcgcca ttattttgca gttcaaagat aagtccatga tgttagttga
    44641 tactcatgct catttgtacg cttccgaatt tgatggagac cggttggaaa tgatccaccg
    44701 ggccattcaa gccggggtcg gccggatcta tttgcccaac gtggatgcgg acacgatacc
    44761 cgccctgctg agcacctgcg cactttcgga catcctttac cccatgatgg gactccatcc
    44821 ctgccaggtg acagccggct tcaccgaaac cctggctgaa ataaaagagg aactgtaccg
    44881 gaattcccgg ctttattacg ccgtcggtga aatgggcatt gatcttcatt gggacaagac
    44941 cacccggcca taccaggaag aagcatttcg ggtccagtgt ggctgggcct gtgaactgga
    45001 tctccccgtg gtgatccata gccgggaagc gacggaggcg gtgctggaac tgctcgagcc
    45061 gatggacccg cggccggcca ggggagtctt tcattgtttc agcggggacc ggcaccaggt
    45121 cgaacgcatc caggccctcg ggaattatta ttttgggatc ggcggggtca tcacttataa
    45181 gaactccggt cttgccgccc tcgtaaagaa tattcccctg gaccggatcg tactggaaac
    45241 cgatgctccc tacctggcgc ccactccttt tcgcggcaag cgcaacgaac cggccttcct
    45301 ggtaaacatt gcggaatccc tgggcagggc cctgggctta ccggtggatg aaattgaaaa
    45361 aatcactacc ggaaatgccc tggccctctt tgaaaggagc ggaaaagtcg aggggtgacc
    45421 ggcaggaatc ccctgtccgc gcccctgcag cagtgaagat cctcccggat ttggatgcta
    45481 actgattttt tagcaatttt gcaccagtca ttcaggcttt acggaaggtt gcaagagtgg
    45541 tttaattggc acgcctggaa agtgtgtgta caccaaaagt gtacctaggg ttcgaatccc
    45601 taaccttccg cttttttttt tactgatccc cggattttcg ttaaaaatcc gggtaagccg
    45661 acaactataa tcccggttgt ctaagtccta aaaagaattt tttataaatg tttggacttt
    45721 gataatattt tctattttta agccttcaaa aaattaaaac attaaactta tgcgtaagat
    45781 tgctttactt tttactcttg ttttggttgc ctttctggca ggcggcctgg agttgatggc
    45841 ccaggagccg gcagcggcag ctgctgcccc ctcaggtgga ttccagatgt taaaagagaa
    45901 attcattgaa ggtggctggc agtggatggt gccgatcctg tttgtattga ttttgggatt
    45961 agctttttgt attgaaaggg tcatcacgct caacgtagcc actataaata cggataaatt
    46021 gctttctaag atagatgacc ggcttcagtc gaacgacatt gaaggagcta tcgaagtctg
    46081 caaagtcacc ccgggcccga ccgcttcgac ccttcttgaa ggattgcggc atgcggaaaa
    46141 aggccccgat gccgtggaaa aggctatatc cgcttttgga ggcatgcag
//
LOCUS       DAOQXT010000027        45533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_917_length_45533_cov_7.633053, whole genome shotgun sequence.
ACCESSION   DAOQXT010000027 DAOQXT010000000
VERSION     DAOQXT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 45533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45533
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_917_length_45533_cov_7.633053"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PKM27_06680"
     CDS             <1..1257
                     /locus_tag="PKM27_06680"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNR06980.1"
                     /translation="KGIDPSLYFDGDLAYILYNSIPPDDKPLYDGHRTIRMYAFDWKN
                     LRVNGEEIILVNGGSDFSKKPVWIEAPHIIKKDGWYYLYCAEGGTFDQHSEVAFRSRS
                     VKGPFIPNPRIPILTQRDLDPSRPNPVTSTGHAEIFQTLAGEWYAVFLGCRPYSGPHY
                     NTGRETFLTPVSWADGWPVINPGFKEVRYSYPVPQMQQNLFTTGVNEFAADQPFLDHF
                     DTRELNNRYSFLRTPSRTWYNFKDKKGYLSIELLPETCSGTANIAMVADRQNHLNGQV
                     STRLRFQTQADHEKAGLLIFQNENHYYFLCKSADRGQPVVQLFKGPGNLHKEQGPELL
                     TSIPLKKNNAGIILKAEVGEGGYSFYFSIKKDKWLLVRSSVDREFLSSHTAGGFTGVM
                     YGMYATSTGQPSVNRAMFDWFEKIGL"
     gene            1590..3938
                     /locus_tag="PKM27_06685"
     CDS             1590..3938
                     /locus_tag="PKM27_06685"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HNR06981.1"
                     /translation="MKKLIWLLVWTGNALISYAQIQAPAGHWQAQWIMPPGRDPDVYG
                     VYHFRKSFELAEIPAQFIVHVSADQRYRLFLNGKPLGLGPARSDPQHWMYDTYDLAPH
                     LYKGKNTLAAQVWNMGEHAPYAQMTVQTGFIVQGHSENEKIVNTNNSWKAFINKAYQP
                     EINDIQKLRTYIVVGAGDRVDASKYPWGWEQTNFTDNPWPQAVKPWFNAKTRGFGTDG
                     NWMLVPRSIPMMEESLLRMHSIRRASQNIKWNEGFLQGKASLTVPKNQKATLLIDQSH
                     LTNAYPQLTVSKGRGASIKMSYAEGMMDADRQKGHRDSIEGRSVMGFDDVFIADGGPH
                     RMFRPLWFRTYRYLQLDIETKGEALILEDLYGMFTGYPFEARGSFVSDHALFGKIWDI
                     GWRTARLCAGETYFDCPYYEQLQYTGDTRIQALISLYVSGDDRLMRKAINEYNQSRIY
                     EGLSQSRYPCNDMQIIPPFSLYWISMIYDYWMHRSDDAWVKSLLNGARDVLDWHEQRL
                     GPDHMNGDLEWWNFVDWTWPSGTPPGREGSSSILSLQYVYTLQQAEKLFRNFGRNAEA
                     DHYLSLAKKIAGSTYSRCWDASRGLMADHPEKKSFSQHANIWALLTDAIPSEDRQKVF
                     KTLLADTSINQATFYFKFYLFELLKKMEAGDLFPELLKPWEEMIGYGLTTFAEKPEPV
                     RSDCHAWSASPNYQFLSIVAGINPSAPGFSEVQISPYLGNQLEVAGEIPHPKGPLKVN
                     YRVSGQKLNARLDLPPGLGGKFIWKGIIYPLRPGMNSFQLDR"
     gene            3943..5376
                     /locus_tag="PKM27_06690"
     CDS             3943..5376
                     /locus_tag="PKM27_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNR06982.1"
                     /translation="MKTEVSPDSPFSEIDRVYRLQKDYLPLARNTGVKERLVKLKRLK
                     KVILLHRNEFQQAMYADYNKPAAEVDIAEIFVLLNELGHVIRRLKIWTAKHHVPTPWY
                     LTGANSYIHYEPKGHALVFSPWNFPIHLTLMPLIYSYAAGNVTVIKPSEFTPYSVEVV
                     KKIIRMVFDEKEAWVAEGGVAVGQYLLSLRFNHIFFTGSPSVGKIVMKAAAEHLSSVT
                     LELGGKSPLIIDETADLKLACSFAATTKFINSGQYCISPDYVFVHESAKDEFVREFIR
                     QAELKFGKNEEDWLTRDYCLMVNGRHLNRVKAILDDAIALGARLEYGGHVNPEKELIS
                     PTVLSGAREGMKILEEEIFGPILLLRTYRDLGEVIQTVNRGETPLSLYLFSRRLATKK
                     RIRKEIQAGTMCINTMAVHYYNYHLPFGGHNASGIGQSHGFWSFCEFSNAKAVMNQWS
                     PFNGIKLVLPPFTRLKKILINWLIRLT"
     gene            complement(5456..6493)
                     /locus_tag="PKM27_06695"
     CDS             complement(5456..6493)
                     /locus_tag="PKM27_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR06983.1"
                     /translation="MTKILVADDEADLESLIKQKFRKQIREQQYEFVFAMNGREALEK
                     LKDHPDIALLLSDINMPEMDGLTLITKIKEINPILKSVIISAYGDMENIRTAMNRGAF
                     DFLTKPVNFEDLEITMEKTIRYVKQLQETVMAIRENKILRMYVDESVLQFMNQKEIEE
                     SILANESIVATVAFIDITGFTKITECEPADEVVKLLNRYFDVISKEIIRHGGYIDKFI
                     GDEVMAVFKGEHHLDQAIEASLDIRQAIENLPEAGTSHYHPKVSIGINSGEMISGNIG
                     SAGIKRLDYTVIGDVVNTAKRIQAVAREGEIIIPESCVEKIKESFKVRKVGEVTLKNK
                     AKPVSVFQVLD"
     gene            complement(6490..6867)
                     /locus_tag="PKM27_06700"
     CDS             complement(6490..6867)
                     /locus_tag="PKM27_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR06984.1"
                     /translation="MKILVVDDERDIQVLFEQRFRKEIRNGEIQLVFAFSGEEALTFL
                     QSSDHEAILILSDINMPGMSGLDLLSRIKTKYKTPPPVVMMITAYGDAENYNRAMSLG
                     ADDFLTKPLDFTILKDKLKNIEV"
     gene            complement(6965..7711)
                     /locus_tag="PKM27_06705"
     CDS             complement(6965..7711)
                     /locus_tag="PKM27_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR06985.1"
                     /translation="MIRSLIIDDEENGRSALQMLVHEYCPDVRVLESCSSAADGLRAI
                     VERKPDLIFLDIEMPLMTGFDLLEKISHLPISVIFTTAFHQYAIKAIRYSALDYLLKP
                     IDPEELIAAVGRYRAQLSKPVADQFRFLMDKLAQKEHTHRKLAIPNMEGFKLVSVDEI
                     VSCEADDNYTHINLKNKSRITASRTLKEIQQLLEDYDLFIRIHHGYLVNLREVSQYIR
                     GEGGYVIMTDGSHFNVSRNKKEALLRHLIR"
     gene            complement(7708..8331)
                     /locus_tag="PKM27_06710"
     CDS             complement(7708..8331)
                     /locus_tag="PKM27_06710"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNR06986.1"
                     /translation="MQALRARMNPHFIFNCLNSINNFILKNEQAKASEFLLKFSKLIR
                     HILQNSMFPLITLDQELEALRLYIEIEALRFHQQFEYRIRLGDGLDSMSIRVTPLIYQ
                     PFVENAIHHGLLPKEGPGHLELEIFEEGESLVCLVRDDGVGRPEQALQAAQPAGHKSL
                     GIRLTTERIGLLPIPDRSIRPVTIQDLTDAMGKPAGTEVRIQTPLQV"
     gene            complement(8332..8778)
                     /locus_tag="PKM27_06715"
     CDS             complement(8332..8778)
                     /locus_tag="PKM27_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR06987.1"
                     /translation="MPIILVADDEEDQEELILQRFSGKPFLDGYEFVFARNGPDAWRK
                     IQERPEIEMALLDINMPGMDGLTLLGKIKDARPEMTSVIVSAYGDMTNIRSAMNKGAY
                     DFLTKPIDLNDLEITLKKSLAQVRQVKETAKTERENQSLKIKTVEL"
     gene            complement(8815..10173)
                     /locus_tag="PKM27_06720"
     CDS             complement(8815..10173)
                     /locus_tag="PKM27_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR06988.1"
                     /translation="MITQFKLFDKVVKGASELKEIVLVYDQNYFSFEFSSLSYDNPAK
                     NQYAYKLEGVDKDWIYSGSRHYAGYTDIKPGHHNFKVKGTNSDGIWNEKGAAISIIIN
                     PPWWKTWWAYTIYGLLFMAAILIIYRMQKQRILRNERQKTQAKEIEQAKEIEKAYRDL
                     KLTKDQLIQSEKMASLGELTSGIAHEIKNPLNFINNFSELNMELIHEVDEDFTPKMEE
                     SEQAGWTHSIKTLKKNSEKINHHGKRIDEIVKGMLAHSRMGNIKKEPVQINALCEDAL
                     KLAYHGFRAKEKAFHASCETRLEANIPVVMVIPQEISRVMLNLINNAFYAVHIKMLQS
                     QKLKVEDSLQMETRYEPSVILSTHFQGNKVSIMVSDNGTGIPDEVIGKVFQPFFTTKP
                     TGEGTGLGLSIAYDIVTKGHGGELKVKSKAGIGTDFEIILPVTAPKPPVPESPHHSKQ
                     VL"
     gene            complement(10662..12308)
                     /locus_tag="PKM27_06725"
     CDS             complement(10662..12308)
                     /locus_tag="PKM27_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNR06989.1"
                     /translation="MQRWLIRETRILYLIFLFSEYQNIQAQNETLRFEHIGIEEGISN
                     EITTAILLDSKGYLWIGTSDGLNKYDGYNFTKYRFDPFDTNSISQNWIYSIFEDSHGV
                     IWVSSFEGMCKFDRATEKFTRYKPSPNARFSDPNISVINEDNEGMIWTGSYASGLCRF
                     NPKTGKFLPDSIDLDYPRSPDNKTIWPFDGVNCIYKDREGTLWVGNTTGLHQIKPTAA
                     KPGQPSGYIIKNYRPDPFNADSLSGMVTSVLEDKAGIIWVATNNGLNSLDQKTGCFKR
                     YQHDPKNMLSMSNNSSYAWGGRGMKEDQQGNLWICTSKGLNKLNKNRTVFTRYFHNAD
                     DPRSLSTDNVTSLEIDRAGILWVGSAAKLNKAKLNDKGFGLRRNDPNDLNSLSNNEVT
                     SIVEDSSGMIWIGTYKGGLNRWDKRTNKFTHFRHDPANPRSLRHDAVRAILEDRNGHL
                     WVGNGDVLSLFNNQMGEFTHYKSNDENYKEEHQLILSITEDREGLLWIATRGDGIRKF
                     DKKARTYLKHYHHNKTDSTGISDYGAMAILADSRDIYGLA"
     gene            complement(12366..13424)
                     /locus_tag="PKM27_06730"
     CDS             complement(12366..13424)
                     /locus_tag="PKM27_06730"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR06990.1"
                     /translation="MNDIHAIGCSQLGNKSRPEVFAYVEKEIKDNLMELSPDVNSKTY
                     GRVTQWFAYALLAKLYLNAEVYTGKPRWNDCIAACNAILTSNKYTLEENFFSNFLVAN
                     EKSGENIFVIPFDINAGLNSFWIQGATLHYSSYSTFGLESGGFNGFCSTAEYLKLFDI
                     QDNRRKMFLVGQQYVNQIKDSAHIQYDPAIDLPLIFDPVITNFSNSSPESRLLGARCA
                     KWEFNKSGSGLMSNDFAVYRLADIILMKAEAQFRNGDVENALMTINQKINRVSIRSRA
                     GLPDFGLSEMNPDGILAERARELSWEGHRRNDMIRLGHYTDARIPEKTPSEGFRTLYP
                     IPKSELDKNPCLVQNPGY"
     gene            complement(13437..13940)
                     /locus_tag="PKM27_06735"
     CDS             complement(13437..13940)
                     /locus_tag="PKM27_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06991.1"
                     /translation="MNKYKYTVMKTYKPFQALLLAVAMMLWGNQACLKLDPEVYDKVS
                     VFWQTDDQIAAGVAPAYSGLRDYAPANAAYVLNETSTDEVIVPVRGGHWADNGVWEKM
                     WKHSWKSDHSYMAEAWQFINSGIARVNLILETVNTLNPKPTGLNAILSELKTVRAFYY
                     YIALDLF"
     gene            complement(13940..16915)
                     /locus_tag="PKM27_06740"
     CDS             complement(13940..16915)
                     /locus_tag="PKM27_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNR06992.1"
                     /translation="MRPGSRLAKYFQSLVLVSEILFFLEGDLYSQSILKGSVKDREGE
                     ALPYATVSIKATKAMAITDSVGHFSIRASSGDLLEVTYVGYRDYQVVLGTENELHIVL
                     SETITNLDELIIVGYGTAKRKDITGAVTRVMPNAFNTGVITNPLQQLQGKVAGMVIVQ
                     PGGDPNGDFIVRIRGASSLEGQPPLLVIDGVAIDDFHKAAATLNPADIESYDILKDAS
                     AGAIYGSRGANGVILVTTKKGNTGRTSLDYNGFVGLERVSNQLNVLSADQWRNATAAI
                     GGGGLDKGGNTDWQKAIAQSGISQSHTIGISGGSDAINFRGSLGYITQEGVILSTGKN
                     VINARLTANQKGLNERLEVRYGINASVINRNFLPDQSTTSQVRLRGSDFFNGVLDFLP
                     VLPAYNPDGTFYQPANVNTINPLYPLTGVYSHFRENYFQGSVKTDYEIFKGFKAGVLG
                     AFSRGNDIYDKFSPVFPGISGKSAAVKSNNNKEVFSGDIHGNYHKSFGNHHLDLTGVY
                     EYNRFVNDAFGVTARGFLVPKLLNNDLSSATDVQTSDIFSAKNEVRLISLLGRAIYTF
                     NNRYILTASFRRDGSSKFGPNHRWGNFPSVAIAWRASNEDFLKDLRWLDNLKLRLSYG
                     FTGNQENLGPYASRLLYGRSGSYLYDGQFLQSYTVMQENNPDLKWEVRKSFNFGVDFS
                     ILKDRVSGTIDLFDDKTSDLLFLYDLPQPPFLTNQVSANAATAINRGIEITLGIGLLK
                     NQRFNWEVLTNFATLKNNITNLSGKFKGTNLSITNRHYGYAEGGGLSGAYITQLQVGY
                     PAGVFWIPVHAGLDTGGHELFNNYNPEGKLAGTSTSYSDQDRVFIDPTPDFTWGITNN
                     LTYGDFDLSFFLRGVQGQKIFANALMNLEAAVNLPSINVGEKALTNGFADQPRPSTYW
                     VRDGSYARLENITLGYNFKNWKSVKNLRVYLSASNLFILTRYDGIDPEIKTEGSQRYI
                     DRNYFPKTRGFSLAIRAGF"
     gene            complement(17234..17737)
                     /locus_tag="PKM27_06745"
     CDS             complement(17234..17737)
                     /locus_tag="PKM27_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06993.1"
                     /translation="MKNLMLIFLLLSILACEDTFTLPEEEIAQTSAFPMVTNQRYILP
                     VDLVTCVPCSNGGKSENVKVSGNLSLNAVVTFHTPQSFTANLYYQALDIKGVGETTGN
                     IYEESSSTQVISFNQAENRKTILNDLTGPKEGMPPVLVMKDSYFVRISESGKVSVQQV
                     NINFDCK"
     gene            18002..18631
                     /locus_tag="PKM27_06750"
     CDS             18002..18631
                     /locus_tag="PKM27_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06994.1"
                     /translation="MKNILFACAFILVAGTGFSQTPIDTYIKEAQAFIAQKNYKQAQL
                     SLQDAINEINNILAKQIAEALPAEINGLQATGESEVNSAGSVMGMGMQISRSYQHPSK
                     PENSAEVMIMANSPMLNALNMYITNPSLMGQGAKSVRVGTKRAVLKSEMEEFTDDKGA
                     SKQIRVTEIQVPISQTLFNIKTRGFATEQEELAFAAKLEMDKIKDLMGE"
     gene            complement(19104..21296)
                     /locus_tag="PKM27_06755"
     CDS             complement(19104..21296)
                     /locus_tag="PKM27_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR06995.1"
                     /translation="MCKKKLLTPWFILVVFFLKAQVSWPPVYQITSDTAVRYDLDSAY
                     WQILEDKPGTLDINQVSGPLLADSFYHFRDRKMEEGIHTCWFRFRIKNGLDKRIGISL
                     SSQASRADFYIFRDSTPGAGMIHLVTGNLYSWKNKDGFKRVSAVPLSLDSMETLRVYF
                     RWHKINSVFSDLPDSLTLRGYHTEKLRQSELEHYESGYVREDIFIPAFLAGFFILAAI
                     LNLLIFLAAKEKLYLYFSLFLLSISFWYNPLFTDILYREYPLTSDFINRLGLSWLFFV
                     LHFIRHYFRSFERFPKWDRFMVYLSVIYLINLVVPPVPVDFFYRPEVIIFKGLFLILY
                     ILLLGVTIVKCLLVKGPARRPFITAALPFILYFLAALIIYALFDQQLKAYGQADALFN
                     YGLGLGIAWLVVMFSWYLYRRYASQQNEITEALLAKEKLEHEKEAERLALIAKQNLEL
                     EQKVKERTADLAQSLENLKATQDQLIHSEKMASLGELTAGIAHEIQNPLNFVNNFSEV
                     NKELIEELRGERLKAKGERSTALEEELLKSIEENELKIIHHGKRADAIVKGMLQHSRS
                     DAGQKELTDINPLCEEFLRLSYHGLRAKDKGFNAFLETDFDPSAGKINIVAQDLGRVL
                     LNLYNNAFYAVSEKRNTNLENFEPKVVVRTRRGADRLFIHIADNGNGIPPQMVDKIFQ
                     PFFTTKPTGQGTGLGLSITYDIIKAHGGSIEVKSEEGRGTEFIIQLPV"
     gene            21508..21951
                     /locus_tag="PKM27_06760"
     CDS             21508..21951
                     /locus_tag="PKM27_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNR06996.1"
                     /translation="MAESWNKKEREKKKRAKKKEKEERRLERKENAKDSNPNDYFSYV
                     DHDGNVTSTPPDPAQKKVFKAEDIEIGVPKQAEPDPEERIHKGVVTFFNESKGYGFIR
                     DLKSQESIFVHINDLEEAVRENSKVEFEVQKGPKGFQATQVKVVR"
     gene            complement(22055..24136)
                     /locus_tag="PKM27_06765"
     CDS             complement(22055..24136)
                     /locus_tag="PKM27_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR06997.1"
                     /translation="MKPGLALKFVVALVLVFAARISQGQRPNIDSLRQELRSTDNDTS
                     RLSLAFLLEDAFTEVNPDSALFYAELIRQLAIKLSLRLEEIYAMGKIGYALQNKGNYA
                     RSLQTYLSAIALAEDPASEKNVLGPQYPAPDEITSRSMPARIQRLDKLSHLHQYLAIL
                     YSNTHNPEKALESAYKALELGNVTQNQRLLCVIHLIMGRLFLVLNKPDSALFAEQTAL
                     DEALRMNFPRYLGSIYLNTGRIHQYNGNAELAAGFYRKALDASLDQNYFRGVVATNLL
                     LSEYFIKAQKRDSAMLHINQALDMAMQMDGPDLLLRTYQALANYYQATPFTDSLVKYQ
                     GLIIRLNEDIFNIKQNQQFQNIGFDEQLRLQALEKEKEELRNRNRTFGLTIGLGALLL
                     VAFVLYRNNLQKQKTNAALEKTLSELKATQAQLIQAEKMASLGELTAGIAHEIQNPLN
                     FVNNFAEINQELFQEIQENLNREEKGQENRPEHGNPVEWVPVKSEILESIRSNQEKIL
                     QHGKRADTIVKSMLEHSRVHSGQKEPTDLNTLSDEFLRLSYHGLRAKEKSFNSAFELV
                     AHPALPLVHAVPQDLGRVLLNLFNNAFYEVYKKSKKGDSGYQPLVRVTTNKLKNGVEV
                     RVEDNGDGIPEAILEKIFQPFFTTKPTGEGTGLGLSLAYDIITKGHSGNLKAVSRPGQ
                     GAEFIIELPLE"
     gene            24192..25172
                     /locus_tag="PKM27_06770"
     CDS             24192..25172
                     /locus_tag="PKM27_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNR06998.1"
                     /translation="MFTKLQIISLLLGLALYPACNKNVQVKKTGKEPAFRVLGYLPAS
                     RDWLAGLEQIDLSKITDLNLAFINPDSSGLFRENEDYLEIVRRAHARRVRVFISIGGG
                     SPLAYLETLMEKEHRTTMISSLTGMAVKYKFDGVDVDLENDLINANYAPFVRELSSSL
                     KRNNLLMTAALASWNGHLIADSTLQLYDFINIMSYDKTGPWNLSRPGPHSPYSMVEQD
                     FVYYNQTRKIPAQKLLIGLPFYGYGFGPGVPQSKSYQEIVNLYPGAESTDSITVPEGG
                     KVYYNGKATIRDKVLFAHRNGARGVMIWQLLGDSRDSRSLLQVIQGAISR"
     gene            25594..25734
                     /locus_tag="PKM27_06775"
     CDS             25594..25734
                     /locus_tag="PKM27_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR06999.1"
                     /translation="MSSVISVRSLSFAHGGKESTEHSYYNHQIFNIISVASVISVRTI
                     SV"
     gene            complement(25773..26972)
                     /locus_tag="PKM27_06780"
     CDS             complement(25773..26972)
                     /locus_tag="PKM27_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNR07000.1"
                     /translation="MLVNDIKREAKKIHKNVVLYRRHLHAHPELSFLERETSGYVKAR
                     LDELGIPWEAMAGTGVVGMIRGESPGDGGPDRVVALRADMDALPITETNTFDFISQNP
                     GVMHACGHDAHTSSLLGTAQILQSVRKKFSGTVKLIFQPGEEKLPGGASMMIRDGVLE
                     NPKPRAVIGQHVMPSLECGKIGFRAGKHMASMDQIIMRIGGKGGHGAQPHQNIDPVII
                     TAHILVALQQIVSRMANPSIPTVLSFGKVIANGAINVIPDEVYVEGTFRTFDEKWRAD
                     AHHRMTKMAEHLAVSMGGTCEMEITRGYPFLVNEEKLTSQLRTFTEDYLGRDKVVDMD
                     LWMAAEDFAYYSQATDACFYLLGVGNPSKGIISSLHTPSFDIDEEALEISTGLMAYLA
                     VRRLGAE"
     gene            complement(27092..28408)
                     /locus_tag="PKM27_06785"
     CDS             complement(27092..28408)
                     /locus_tag="PKM27_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR07001.1"
                     /translation="MSESSDHGYFRGVVASHLALADLFKSSGQADSIKYHITQGLTVT
                     QTLNAPDLFLRCYTAMADYYKLTGQSDSTVKYQSLIIKINESLFGTKLLQQFQNIDLD
                     AQQRQQEVEATRKEYQNRLQTNSLLGGLSTILIVAVLLWRSNLQRKKNNSLLAAQNKE
                     IETALDHLKSTQAQLIQSEKMASLGELTAGIAHEIQNPLNFVNNFAEINKELLEEMEG
                     ERRKEKENRDERAESYILTTLKENQDKILQHGKRADAIVKAMLLHSQRSSGTKQPTAI
                     NSLAEEYFKLAYHGYRAKDQNFSAELRLDLDPDLPLVNVIPQDIGRVLLNLSNNAFWA
                     CATKSRGGSEAPPGSAAYSPTITLSTKKRGEEIEIHIRDNGSGMPQNIIDKIFQPFFS
                     TKPAGQGTGLGLSLAYDIIKAHRGSITVESKEGEGTEFVIHLPVAG"
     gene            complement(28335..29162)
                     /locus_tag="PKM27_06790"
     CDS             complement(28335..29162)
                     /locus_tag="PKM27_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR07002.1"
                     /translation="MARIVFLFLLLPINAVSAQPAIFHSLKYEISRENNDTLRLLLLE
                     RIANEYSEINPDSAYHYASEVPELARKLNLRLEEVVGLGEMGYAQLNLGNYPRSLRSL
                     LSGIAIAKDPASEKIILPSRFPPNDDFMDRTVSPADQRLTKLARILQFTGILYGNTGN
                     YEKAVTYFKEAISLAEQSNNLRLLSITHTTLGRIYLASNNPDSALSSLQKGYDYAIRA
                     GYHRYLGSNLLNLGRVYLAMKTAGIGQNLFPEVHVGEFRSRLFQRGGRQSPRPGGFI"
     gene            complement(29351..31339)
                     /locus_tag="PKM27_06795"
     CDS             complement(29351..31339)
                     /locus_tag="PKM27_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR07003.1"
                     /translation="MGKSLPASGALYKNNPWSWVFMLSLLLLAWIALSQTNREDSLFQ
                     YLSKSGQDTNRVLTLIQLADLYRFARPQEAVDLMREAQDLAVKLNFKKGEVQVMNLLG
                     ETARVNGEYTRSLEIQLRALEMSRSLKNALAEANSLTYIGLTYTEIGDYPPGLDFLNQ
                     AFVLHDRLNMPVNGSFDLSNIGFTYEKMGLLDSALFFQLKAREYLKQTYHIALENLIL
                     TRLGQLKSLMGQPGEALAYGREALNRAQRTGDMRNQSGALYRMAEAFNALNQADSSLH
                     CARLALSISQSLEQRNISLDLYNLLSRLHFDGGRMDSAYYFQKLSIAVKDSLFGPQKI
                     RELQRVSFNEFQKQQKSEAERLNFKNRVKIYSLLALVLVFSGLGIVLYRNNMQKQKAN
                     QILAKTLNDLQTTQSQLIQSEKMASLGELTAGIAHEIQNPLNFVNNFSDVNRELIDEA
                     EQEMNKGNVPGVRNLLNEIRENELKIITHGKRADAIVKGMLQHSRTGKSEAEATDLNG
                     LADEYLRICYHGYRAKDKSFNVELKTEFDPSLQKISIVPQDIGRVILNLLTNAFDAVK
                     EKKRRQPDQYEPAVLLRTRKEGNQVILQVKDNGLGIPPSLIDKIFQPFFTTKPSGQGT
                     GLGLSLAYDIVKAHKGELWVESREGEYSEFNLRLPVIH"
     gene            complement(31402..33519)
                     /locus_tag="PKM27_06800"
     CDS             complement(31402..33519)
                     /locus_tag="PKM27_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR07004.1"
                     /translation="MPLGKYYFKFTRGNWNNVETTAFSEDIEDRVIEIKQDTTVQLRI
                     EGWMDDYINLAGLADSTAILVGLRKSYYYREIHLDSSYKYALHSLELSRRSKDKNAEI
                     QSMISLGAVFKEQGNSVKALELLSQVLPIAELQQDSFQLVLVNQSIGDIFGTIAEYDK
                     AKYYYLKAIKYTSSAEDIRAFWRSGLFLNLARVYYHTGKLDSASYYANLNLELSPASG
                     AFLLLGDIDREGRNQDLAMTHYRQAVDLESKSSISSFSLTEVYDRLAQTFFEAGQSDS
                     AFHYARLSLTIANRVKNPSAIVNANALLSKLFESRHGFDSAFYYQKVAIMTNDSIFTR
                     EKEKQIHTLVFNEQLHQQEMAAQNAKLQAKAWIYSLAGGLLVLLLIAGLLSRNIRQRK
                     ATNILLKNQKDEIEIQKRNVEETLSELKSTQAQLIQSEKMASLGELTAGIAHEIQNPL
                     NFITNFIEINQELLDEMQEHRKRNSGFRRPEAGEEKSELIETEILNTIRKNQAKILQH
                     GKRADSIVKSMLQHSRSVDPASKGKKEPTDINALAGEYLRLAHHAFQAGLRAKDMTFK
                     AAFVLDLDPDLEKFNVVPQDMGRVLHNLINNAFWAVTEKQNQSGTSEYTDGLEYKPTV
                     TVKTQKINDRMELRVIDNGMGIPQNILDKIFQPFFTTKPTGQGTGLGLSLSYDIIKAH
                     GGDLRVETNEGEGSSFIISLPKS"
     gene            complement(34346..45505)
                     /locus_tag="PKM27_06805"
     CDS             complement(34346..45505)
                     /locus_tag="PKM27_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR07005.1"
                     /translation="MKLTNGILIADFTREPQVETEIILLKIGAAFDLAYKRFLDLQKA
                     EGQARESRIQLALERVRARTMAMQESSELPEAANLLFHQVKTLELPAWSAGYCIWNED
                     KSAVTLWMSTEGALQPPFTAPTTEDELFIQMRKGHEHRKPLHRVEMGGEKLVKHYRYM
                     RTLPVVGEILDSVIKAGYPLPDFQIMHYAYFSAGFLFFITYEPVPAAHDIFIRFARVF
                     DQTYTRFLDLQKSEAQAREAQIEAALERVRSSSMAMHQSSDLHQVIKVVTEQLCSLRL
                     KFDVANFAKVDEAGNWDLWLSTPAQAYPAQLFVPYLDHPIFNRVLEMRKNQLDHISDV
                     YDQKVKNVFFRHFFKNSEAKHSPESRKKQVLNRKGFARTLFYFKDIWFAISNYEGIPF
                     SAEEVGIFRRFAKAFEQSYIRFLDLQRAEAQAREAQIEAALERVRSRSMAMHRSDELK
                     DVIRLVLQQFGQLNINAEHAGFYIDYKAHEDMHIWLADPKLEPFYAVIPYFDTPTWNS
                     FLEAKANGTILHTDLLDFKTKNKFYRSLFKLFTIPEEAQKFYLKCKGLAVSTVLLDNV
                     GLYIENFSAIPYTDEENNLLIRFGNVFQQTYTRFLDLQKAEAQAREAQIEAALERVRS
                     RTMAMHTSSELIQTAELLFDQLKQLGAELQGVAFAICDKNSVMVQKWTSIGVFNHPYN
                     IEPGEERMYEAWKNQAALYEEVYEGERQKKYYEAFMQIPAFKQGIQKFIDSGHPLPAW
                     QKNHAVPFKFGYLLFITTKPFNETQIFLRFGKVFEQTYTRFLDLQKAEAQARESQIEA
                     AMEKIRSRSLAMHHSSELSAVIAVFFEKLSELNVLLGTVGIQLFDQRTGDSVLWVGNA
                     LQEPQMINLPHDDGVMQQDTYLKDGWQAFSTGQAIINRFYSRQQKDRFIDFVFSHNDL
                     IRIPENARTVLRDLDDYYVCLIAESHSALFADSFTGQPYPEENLDVIRRAARVFNQAY
                     IRFLDLQNAEVQAKEAQIQLALERIRARTMAMQKSDELPGTSFLLFQQMKELGLTAVQ
                     NSIGIVKEDAGVVELSTTVQGHPEPKTLYVPIDDPHVMAKAVAAWKAKRSSLTLEFHG
                     RELKEYNKLRNRFLEIKVNFPEDHWIVNIVFFSKGWLSFSSDKNIPGETLLLMERFAG
                     VFDGTYTRFLDLQKAETQAREAQIEAALERVRARAMAMHSSQELNEVVHELRKQMGLL
                     GQKDLETCVIHLNEESSDYIHSWAAIRPPEGEGEILVDSTARVPKKGLRIIEEAMEAY
                     SSHRQDYILLNEGDKLKQWFSFLEKESPRSYSRLVDSVQGKIADLRAFWSFADFTGGS
                     LLMVTMEAPDDATRGLLRKFANVFGLAYRRFADLKQAEAQAKEAEIQLALERVRARSL
                     AMHHTSELQEVVNVTAQQLLGIGMDIDGGVFICINAEVGTGLSIWASGGMADYVEKIV
                     VPVLNKPIFTRIRDAIKKGNTFLIESFTDQEKRTFFDHLFQYEPWQSLSQERKEALLS
                     RTGGFARSVVISRHTSISITNHHGKAFSEQENEVLKRFGKVFEQSYTRFLDLQKAEAQ
                     AREAEIELGLERVRARAMAMQSSEELSTLIGTVFTELTKLDLALTRCIIWVFEPATHA
                     ARWWMANSEEPSRPMSFYIKHHEHPAYLTFVREWKNQNVKFVYDLKGQDKINWDDLLF
                     NETELSNLSDAVKNGMKAPERVLLSASFNNFGGINVASLEPLSNEHFDILLRFAKVFD
                     LTYTRFLDLKNAEAQAREAQVEAALERVRSQAMAMRQSGDLLDIVVTMRNEFTRLGHE
                     AHYFWYMRWLPDRYEKAMTSGDGTRIGMIMSLPRHIHGDIPLVAHWEKSEEPALVYAM
                     DVDAALNYVDKMVALGDFELVDPHAPSHDDIRRIQGLTFIMARTMHGEIGYSLPGVVS
                     NPPEEDLKTLVRFANVFDLAYRRFEDLKEAENRNRETQIELALERTRTKTMLMQHSDE
                     LLEISKVFHQQLLLLGIDSEFSFVWLPNEEKGDHLFWATWMQEKDGTAAFHTRAITYP
                     LDLTEPYTAKCFEDWRSGVPVHEHFIAPENIETFFASWEELLEGAEGLKPVFFPEGIY
                     YTEAFMKYGCFGIDIRRPLSQQEQSILHRFAIEFERTYTRFLDLQKAEAQAKEAQIQL
                     ALERVRARTMAMHQSDELREVVSLLFKQIKGLGFEAFMCSILLLDESKRGYQEFMSSD
                     EQSVLPQIYKVPYLEDPFHDKFMAAFNEGLSYKVFELGGKEKREYDKKVFTLTDFRFL
                     PEESKQMMMDTLRCSICCAYTKHGCIVAVSDQELDSGHSKVLQRFAKVFDQTYIRFLD
                     LQRAEEQAREAQIEAALEKVRSRSLAMHKSDELKEVVVVVFEKLKELGLTFDVAGIQL
                     YTGGTRDIVQWVAAPGLASAPVLANLPFIEDDYQDSEIIRDVWTAKESGKNFSKHYSF
                     QEKNKFFAYAHRHNDIDTIPEVARQIQMQAPGYTQSLVAEKHSALWVDSYSGQTISEE
                     EFNVLGRFARVFEQAYVRFLDLQKAEAQAREAQIEASLERVRARTMAMHRSEELVEVS
                     ELLYNELRGLGVSRFINCGYVEIDETNNIQNAWMTVVDGSGVNAVRLPLTGEQVFNER
                     YDAWKRKEKIFHQSVGGELLKRHIEFGTQHYRDTEIDEFVRTRFADPTIFYCSNFSQG
                     YLHIITDALLSVQDELLLIRFTTVFEMTYKRFLDLQKAEAQVRESQIQLALERVRART
                     MAMQKSNELPEAANILFQQVQSLGFPTWTAGYCIWDEDKKAITLWMSRPWGDIQPPFR
                     VPLDENPTFIRYLEAHNEGLELYIDEISGKSLEENYKYLLTLPVVGEQLESILAEGHP
                     LPTYEVDHCAYFSKGFLLFITYQQVPEAHDIFKRFAKVFEQTYTRFLDLQKAEAQARE
                     AQIESALERVRSRSLAMHRSDELEQVAGSLFEAVVGLGIAMDGALLFVFNRENKNIRL
                     WIATVQLTKPVFIDLPHEEVMKDNQVILDYWNTIEIGGQLLNRVYSVEAKNDYFRYVH
                     RHNGSKIPDQLKQLQLELPAWNFSFVAEKNTMLGLDSWSGHLFAEADLNTLRRFSRVF
                     EQAYTRFLDLQRAEAQTREARIEASLERVRSKAMAMHSSQDLAATIQTFYRELNSLNL
                     TPRRCGVGLIDRETRMSELSTMNTTSDGESIEILGKLVLENHFVLEGVYEHWLAQEEY
                     HPVLRGNEIREYYQLLRPQIQFPEYPQDAVQYGHFFFFDEGGVYAWTEKPLAEDELKI
                     YRRFTSVLSLTYKRYRDLRNAEARAKEAVRQASLDRLRAEIASMRTAADLDRITPLIW
                     KELTTLGIPFVRCGVFIMDEPNNQIHTYLSTPDGRAIAAFHLPLDNPGNLGEAVQYWR
                     EHKAYVTRWVDTDFQAQADLLAHLGAVDSRDEYLSNVPADGIYLHLSPFRQGMLYVGN
                     TAPLSDTELDLVQSLSDAFSVAYARYDDFRQLEMAKKDVENALAGLKQAQQQLVQSEK
                     MASLGELTAGIAHEIQNPLNFVNNFAEVNAELIEEIRQAIQAGNSAEVLHLTEDLKQN
                     LIKINFHGKRADGIVKSMLQHSRSGDAGGKNKKEPTDLNKLAEEYLRLAYHGLRAKDK
                     SFNAALHTDFDDTVGLIEVSGQDLARAILNLINNAFYAVQDKEKRMKSRGEPFTPQVS
                     LITKRTGQTVEIRIKDNGPGIPEEVRDKIFQPFFTTKPAGQGTGLGLSLAYDIVKAHG
                     GNIHLNSTPEGSDFQILLPST"
BASE COUNT        11437 a        11133 c        10489 g        12474 t
ORIGIN      
        1 aagggcatcg acccctccct gtactttgac ggggacctgg cctatatcct ttacaacagc
       61 attcctccgg atgacaaacc gctttacgac gggcaccgga ccatccggat gtatgccttt
      121 gactggaaaa atctgagagt caacggggag gaaataatcc tggtgaacgg aggttcggat
      181 ttcagcaaaa agccggtgtg gatcgaggcg ccccatatta ttaaaaaaga cggatggtac
      241 tacctctact gcgcagaagg cggcaccttt gaccaacaca gcgaagtcgc ttttcgcagc
      301 aggtcggtga aagggccttt tattcccaat ccccgaattc cgatcctcac gcaaagggac
      361 ctcgaccctt ccaggcctaa tccggtaact tctaccgggc acgccgaaat atttcaaacc
      421 ctggccggag aatggtacgc ggtattcctg ggctgcagac cttattcggg gccgcattat
      481 aatacaggga gggagacctt tttgaccccg gtctcctggg cggacggatg gcccgtgatc
      541 aatcccggtt tcaaggaagt aaggtattct tatccggtac cgcaaatgca acagaactta
      601 ttcaccaccg gcgtcaatga atttgcggcc gaccagcctt tcctggacca ttttgacacc
      661 cgggaattga ataaccgtta cagtttcctg cgcacccctt cccggacctg gtataatttt
      721 aaagataaaa aagggtacct gtccatagag ttattgccgg agacctgctc gggaacggcc
      781 aatatcgcca tggtggcaga ccgccagaat cacctgaacg gccaggtttc cacccggctg
      841 cgttttcaga cccaggcgga ccatgaaaaa gccggcctac tgatctttca aaacgaaaat
      901 cattattatt ttttatgcaa atccgccgac cggggacagc ctgtggttca attgttcaaa
      961 gggccgggga atctccacaa ggaacaggga ccggaattgc tgacttccat cccattgaaa
     1021 aaaaacaatg cagggatcat cctcaaagcg gaggtgggcg aaggaggtta ttccttttat
     1081 ttttccatca aaaaagacaa atggcttctc gtccgttctt ccgtggacag ggaattcctg
     1141 agcagccata cggcaggtgg attcaccggg gtcatgtatg gaatgtacgc tacatcgacg
     1201 ggccagccat cggtaaacag ggctatgttt gattggtttg agaagatagg gttataggat
     1261 tcataggatt tgtaggattc ataggatttg taggattcat aggatttgta ggattcatag
     1321 gatttgtagg attcatagga tttggaggat tcataggatt tggaggactt gtaggaatta
     1381 caagattgag ggataggatg aatgagttgt ggaattgtcg aatggtccta agaatactaa
     1441 atccaaatac cataaaggga gtcaggagga aaggaaatat aaagcctcag gctgtcccga
     1501 gagccgatta ctaacttact gaccacgggc taactgacta actgactaac tgaccaacta
     1561 acccgtatct tgtcctcaat ctcagtttca tgaaaaaatt aatctggttg ttggtttgga
     1621 cagggaacgc cttaatctcc tatgcacaaa ttcaggcccc tgccggccat tggcaggcgc
     1681 aatggatcat gcccccgggc agagacccgg atgtttacgg agtctatcat ttcaggaaaa
     1741 gttttgagct ggccgagatc cccgcgcagt ttattgtcca tgtcagcgcg gaccagcgtt
     1801 accgtttgtt tttaaacgga aaacccctgg gcctcggtcc ggcccgcagc gatccccagc
     1861 actggatgta tgatacctac gacctggctc cccacctgta caaagggaaa aacacccttg
     1921 ccgcccaggt atggaatatg ggagaacatg ctccttacgc gcaaatgacc gtacagaccg
     1981 gtttcatcgt acaggggcat tccgagaatg aaaaaatcgt aaatacaaac aacagctgga
     2041 aagcctttat aaacaaagct taccagccgg agatcaacga tatccagaaa ctcagaacct
     2101 atatcgtagt gggcgccggg gaccgggtgg atgcctcaaa atatccctgg ggctgggaac
     2161 aaaccaactt tacagacaac ccatggcccc aggcggtcaa gccctggttc aacgccaaga
     2221 cccgtggatt tggcacggat ggcaattgga tgctggttcc gcggtccatt cctatgatgg
     2281 aggagtcctt gctgcgcatg cattccattc ggagggccag ccagaacatc aaatggaatg
     2341 agggcttcct ccaggggaag gcctcgctca cggtcccaaa aaatcaaaaa gccaccctgc
     2401 ttatcgatca atcgcatctg accaatgcct accctcaact cacggtgagc aaaggccggg
     2461 gagccagtat aaaaatgtct tatgcggaag gcatgatgga tgccgatcgc cagaaaggtc
     2521 accgggacag catcgaagga aggagtgtca tgggcttcga cgatgttttc atcgctgatg
     2581 gagggccgca caggatgttc cgtccgctct ggttccgcac ctaccggtac ctgcaactgg
     2641 atatcgagac caagggcgaa gcccttatcc tggaagatct ttacggcatg ttcaccggat
     2701 atccttttga agccaggggc agttttgtgt ccgaccatgc cttgttcggc aagatatggg
     2761 atataggctg gaggacggcc cgcctttgcg ccggggaaac ctatttcgat tgcccttatt
     2821 acgaacaatt gcagtatacc ggggataccc ggatccaggc gctgatttcc ttatatgtct
     2881 ccggggatga ccggctgatg cgaaaggcga tcaatgagta taaccagagc cggatctatg
     2941 aaggcctcag ccagagccgg tatccctgca acgacatgca gattattcca cccttttccc
     3001 tctactggat cagcatgatc tatgattact ggatgcaccg cagcgacgat gcctgggtga
     3061 agtcattgct caatggtgcc cgggacgtcc tggactggca tgaacaaaga ctgggtcccg
     3121 accatatgaa tggagatctg gaatggtgga attttgtgga ctggacctgg ccctcgggca
     3181 ctcccccggg gcgtgaaggt tcctcatcca tcctttccct gcaatatgtg tacaccctgc
     3241 agcaggccga aaaattgttc cggaattttg gcaggaacgc cgaggcggac cattacctat
     3301 ccctggccaa aaaaatcgcc ggctccacct attcgcggtg ctgggatgct tccagagggt
     3361 tgatggccga ccacccggag aaaaaatcat tcagccagca tgccaatatc tgggccttgt
     3421 tgacggacgc catcccatcc gaagaccggc aaaaggtttt taaaaccctc cttgccgata
     3481 cttccatcaa ccaggccact ttttatttta agttttacct gtttgaatta ttgaagaaaa
     3541 tggaagcggg ggatctgttt ccggaattgc tcaaaccctg ggaagaaatg atcggctacg
     3601 ggttgaccac ctttgcggaa aaaccggagc cggtgcggtc cgattgccat gcctggtcgg
     3661 cttctcccaa ttaccagttt ttgtccatcg tagcggggat caatccatcg gctccgggat
     3721 tcagcgaagt gcagatcagc ccctacctgg gcaatcagct tgaggtggcc ggagagatcc
     3781 cccacccaaa aggaccttta aaggtaaact accgggtgag cggccaaaaa ctgaatgccc
     3841 ggctggacct cccgccgggc cttgggggta aatttatatg gaaaggcatt atctatcccc
     3901 tccggccggg gatgaattca ttccaactcg accgttgatc ccatgaaaac cgaagtcagt
     3961 ccggacagcc cgttttcgga aatcgaccgg gtttaccgct tgcaaaaaga ctatctgccg
     4021 cttgcccgga ataccggcgt caaagagcgt ctggtaaaac tcaaacggct gaaaaaggtg
     4081 atcctgcttc accggaatga atttcagcag gccatgtatg ccgattacaa caagccggcg
     4141 gccgaagtgg atatcgccga aatctttgtc ctgctcaatg aattgggcca tgtcatccgc
     4201 cggttgaaga tctggaccgc caaacaccat gtgcccaccc cctggtacct caccggagca
     4261 aacagttata tccattatga gcccaagggg catgcccttg ttttttcacc ctggaatttt
     4321 cccattcacc ttacccttat gcccctgatc tattcttatg cggcgggcaa tgtcaccgtt
     4381 atcaaacctt cggaattcac gccttacagc gtggaagtgg taaaaaaaat cattcgaatg
     4441 gtcttcgatg aaaaagaagc ctgggtggcg gaagggggcg tcgccgtggg acaatacctg
     4501 ctcagcctcc ggttcaacca tatctttttt accggcagcc cttccgtcgg caagatcgtg
     4561 atgaaggccg ccgccgaaca cctgagttcg gtcaccctcg aactgggcgg taaatccccg
     4621 ctgatcattg atgaaaccgc cgaccttaag ctggcctgtt cttttgccgc caccaccaag
     4681 tttataaaca gcgggcaata ttgcatttcc ccggattatg tgtttgtcca cgaatcggca
     4741 aaagatgaat ttgtccgtga attcatccgg caggcggaat tgaaatttgg caaaaatgag
     4801 gaggactggc tcaccaggga ttattgcctg atggtgaacg ggcggcacct gaacagggtc
     4861 aaagccatac tggatgatgc gattgccctg ggagccaggt tggaatacgg tggtcatgtc
     4921 aatcctgaaa aggagctcat cagcccgacc gtcctctccg gcgcccggga aggcatgaag
     4981 atcctggagg aagagatctt cgggcccatc ctgctcctgc gaacctaccg ggacctcggg
     5041 gaggtgatcc agaccgtcaa ccgcggggaa acccccttat ccctgtacct gttcagccgc
     5101 aggctggcaa ccaaaaagcg gatacgaaaa gaaattcagg cggggaccat gtgcatcaac
     5161 actatggccg tacattatta caattaccac ctgcctttcg gcgggcacaa tgcaagcgga
     5221 atagggcaga gccatggttt ctggtccttt tgtgaattct ccaatgcaaa ggcggtgatg
     5281 aatcaatgga gcccgttcaa cgggattaaa ctggtacttc ccccttttac ccggcttaaa
     5341 aagatcctga tcaactggtt gatcaggctg acttagggga ttaaagtgaa ggtgtgactc
     5401 agagtcacgc cttcacttta agctgactca aagtcaaccc ttcacttcaa gcaattcaat
     5461 ccagtacctg gaacaccgat accggctttg ctttgttctt taaggtcact tcgcccactt
     5521 tccggacctt gaaagactcc tttatttttt ccacgcagct ttccgggatg atgatttcgc
     5581 cctcccgggc caccgcctgg attctcttgg ccgtattcac tacatccccg atgacggtat
     5641 aatccagccg tttaatgccg gcagacccga tattgccgga gatcatttcc cccgaattga
     5701 tcccgatgga gactttggga tgataatggc tggtgcccgc ctccggcagg ttttcaatgg
     5761 cctggcggat atcgagggag gcttcaatcg cctgatcgag gtggtgttct cccttaaaga
     5821 ccgccatgac ctcatcgccg atgaatttgt cgatataccc cccatgcctg atgatttcct
     5881 tgctgatcac gtcaaaatag cggttgagca gtttgaccac ctcatcggcc ggttcgcatt
     5941 cggtgatctt ggtaaatccc gtgatgtcga tgaaagccac ggtagccacg atggattcgt
     6001 tggccaggat cgactcctcg atttcctttt gattcataaa ctgaaggacc gactcgtcca
     6061 catacatccg gaggatttta ttttcccgga tggccatgac ggtttcctgg agctgtttga
     6121 cgtaccggat ggttttttcc atggtgatct ccaggtcctc gaaattgacg ggtttggtca
     6181 gaaaatcaaa agctccccgg ttcatggcgg tccggatgtt ttccatatct ccataggcgg
     6241 aaatgatcac ggatttcagg atgggattga tttccttgat tttggtgatc agcgtgagcc
     6301 cgtccatttc gggcatattg atgtcactca gcaggagggc gatatccgga tggtccttca
     6361 atttttccag ggcctccctg ccattcatcg cgaaaacaaa ttcgtactgc tgctcccgga
     6421 tctgttttct gaacttttgt ttgatcaggg attccaggtc cgcttcatca tccgccacca
     6481 ggatcttggt catacctcga tattcttcaa tttgtctttc aatatagtga aatccagtgg
     6541 tttcgtgagg aaatcatccg caccgaggga catcgcccgg ttgtagtttt cggcatcccc
     6601 gtaggcggta atcatcatga ctaccggagg aggggtttta tatttggttt tgatcctgga
     6661 gagcaggtcc agcccgctca ttcccggcat gttgatgtca gagaggatca ggatggcttc
     6721 atggtcgctt gactggagga aagtgagcgc ttcttccccg gaaaaggcaa agaccagctg
     6781 gatttccccg ttccggatct ccttgcggaa ccgttgctca aaaaggacct ggatatccct
     6841 ttcgtcgtct accaccagga ttttcatgta gttttggatt gattatttgg gatgtaaaat
     6901 tagtcagatc ctgcttaagt tgggccctgt tttttttcgg gacggtttac tgccaaaaaa
     6961 atgcttatcg tatcagatga cggagcaggg cttccttttt attccttgaa acattaaagt
     7021 ggctgccgtc cgtcataatc acatagccgc cttccccgcg tatgtattgg cttacttccc
     7081 tcaaattgac caggtacccg tgatggatcc ggataaacag gtcataatcc tcaagcagtt
     7141 gctggatttc tttcaaagta cggctcgccg tgatcctgga cttgtttttc aggttgatgt
     7201 gggtgtagtt gtcatccgcc tcgcaggaaa cgatttcatc cacggatacc agtttaaacc
     7261 cttccatatt ggggatggcc agtttccggt gggtatgttc cttttgggca agtttgtcca
     7321 tcagaaaccg gaattgatcg gctaccggct tcgaaagctg agcccggtaa cgtccaacgg
     7381 ctgcaatgag ttcttcggga tcaatgggct tcaacaaata atccagtgcg ctgtaacgga
     7441 tcgctttgat ggcgtattga tgaaaagcag tagtaaagat cacggaaatg ggcagatggc
     7501 tgatcttttc caggaggtca aagccggtca tcagaggcat ttcgatgtcc agaaagatca
     7561 ggtcgggttt ccgctctacg attgcgcgca aaccatctgc cgccgaagag caggactcca
     7621 gcacccgtac atcggggcaa tactcgtgga ccagcatctg caatgcagaa cggccattct
     7681 cttcgtcatc gatgatcagc gaccggatca tacttgcaag ggggtttgaa tccgaacttc
     7741 cgtacctgcc ggtttaccca tcgcatcggt aaggtcctgg atcgtgaccg gccggattga
     7801 ccggtccggg atgggcagca agccaatgcg ttcagtggtt aatcggatgc ccagggattt
     7861 atgacccgca ggttgagcgg cttgtaatgc ttgttctggc ctcccgacgc catcatcccg
     7921 gaccaggcat accagggact ccccttcttc aaaaatttcg agctccaggt gaccgggacc
     7981 ttctttgggg aggaggccat ggtgaatagc attttccaca aaaggttgat aaatgagcgg
     8041 ggtcacccgg atcgacatac tgtccagtcc atcgcccaaa cggatccggt attcaaattg
     8101 ctgatggaag cgcagggctt cgatttcaat atacaggcgg agggcttcca gttcctgatc
     8161 cagggtgatc agcgggaaca tggagttttg caggatatgc cggatcagct ttgaaaattt
     8221 caaaaggaat tccgaggctt ttgcctgttc gtttttcagg atgaaattgt tgatggaatt
     8281 caggcagtta aagatgaaat gagggttcat ccgtgcccgg agcgcctgca tttacagctc
     8341 caccgtttta attttcaggg attgattttc acgctcggtt ttggcggttt ccttgacctg
     8401 cctgacctgg gccaggctct tcttcagggt gatttcaagg tcattgaggt caatgggttt
     8461 ggttagaaaa tcgtaagctc ctttgttcat ggcgctccgg atattggtca tgtctccata
     8521 ggccgataca atcacggaag tcatctcagg cctggcgtcc tttattttac ccaataaggt
     8581 gaggccatcc atccccggca tattgatgtc caaaagcgcc atttcaatct cgggcctttc
     8641 ctgaattttt ctccaggcat caggtccatt ccgggcaaaa acaaattcat aaccatccaa
     8701 aaacggcttg cctgaaaatc gctgaaggat aagttcttcc tggtcttctt cgtcgtctgc
     8761 tacaaggata atgggcattt gtggtttggt tttcaaatta ttaaaaagaa ttgttcaaag
     8821 gacctgcttt gagtggtgag gggattccgg cacgggtggt ttgggtgcag tcaccggcaa
     8881 tataatttcg aaatccgtac ctatgcctgc tttggatttc acctttaatt cccccccgtg
     8941 ccctttggta acgatatcgt aggcaatgga aagccccaat cccgtgcctt ctcccgttgg
     9001 tttggtggta aagaagggct ggaacacttt accgatgact tcatccggga ttccggttcc
     9061 attatccgat accataatcg aaaccttatt tccctggaaa tgggtgctca ggatcactga
     9121 tggttcatac ctcgtttcca tctggaggga atcctccacc ttcagttttt gactttgaag
     9181 cattttaata tgcaccgcat aaaaggcatt attgatcaga ttgagcatga cccggcttat
     9241 ttcctggggt attaccataa ccaccggtat attggcttcc aggcgggtct cgcagctggc
     9301 atgaaatgcc ttttctttgg cgcggaatcc atggtaggcc agtttcaggg catcctcgca
     9361 taaagcgttg atttggaccg gttccttttt gatattgccc atccgggaat gggcaagcat
     9421 ccctttgaca atctcgtcga tgcgtttccc gtgatggttg atcttttctg aattcttttt
     9481 aagtgtttta atggaatggg tccaccctgc ttgttccgat tcctccattt ttggggtaaa
     9541 gtcttcatcc acttcatgga tcagttccat attgagttcg gaaaagttat tgataaaatt
     9601 cagggggttt ttgatttcat gggctatgcc ggaagtgagc tcgcccaaag aggccatttt
     9661 ttcagactgg ataagctgat ccttggttaa tttaagatcc ctatacgctt tttcgatttc
     9721 cttggcctgc tcaatctctt tggcctgggt tttctgcctt tcgttgcgga ggatacgttg
     9781 cttttgcata cggtaaataa tgagtatggc tgccataaaa agcaacccat aaatggtata
     9841 tgcccaccag gtcttccacc agggtgggtt aataattatc gaaatggcgg cgcccttttc
     9901 attccaaatg ccatcgctgt ttgttccttt caccttgaaa ttatggtggc ctggtttaat
     9961 atccgtatag cctgcatagt gtctcgatcc gctataaatc caatctttat ccacaccttc
    10021 cagcttatag gcatactggt ttttagccgg gttgtcataa cttaaagagg agaattcgaa
    10081 agaaaaataa ttctgatcgt aaaccaaaac aatttctttt aattcactgg cgccctttac
    10141 tactttatca aagagcttaa attgggtaat cactacgggt gcaatagaac tatccgcttt
    10201 aatttgcagt ggatcaaaaa agttaaatcc attattgctt cccaagtaca aggtgccatc
    10261 cttgccctta aaatgtccag agcgtaaact ttcactcaca gcaaaaatat tgccttgcaa
    10321 tccatctcta taatagtaat tagtaaatgt tttttgaaca ggatcaaacc ttgaaagccc
    10381 attttgagtt cccagccaga gatagttatc gttgtcttcc aaaatagaat atacggtgtt
    10441 atcagcaaga ccatgtttat ctgtaaaggt tgtaaacttt ccagtttgat aatcgaaacg
    10501 gcacaagccc cctccccagg taccaagcca aatatttcct ccggtatcct cgtacaaacc
    10561 gaggacacta ttagaactga tgctcgctgt atccttcgaa tcatgtttaa aatgagtaat
    10621 ggagtctgtt cgcttattca gccggtcggt agcgatcgtg cttaggccaa tccatatata
    10681 tcccttgaat ctgcaaggat agccatggcc ccataatcac tgatgcccgt ggaatcggtc
    10741 ttgttatgat gatagtgttt taagtacgta cgtgcttttt tatcgaattt cctgatgcca
    10801 tcaccccggg tagctatcca aagcagcccc tcccggtctt cagtaataga taaaatcaac
    10861 tgatgttctt ctttataatt ttcgtcgtta cttttataat gcgtgaattc gcccatttgg
    10921 ttattgaaaa gggataatac atctccattg cctacccaaa gatgtccgtt tcggtcttct
    10981 aaaatcgcac gaacggcatc gtgtctcagt gatctgggat ttgccggatc gtgtctgaaa
    11041 tgagtaaact tatttgtcct tttgtcccaa cggttcaatc ctcccttata agttcctatc
    11101 caaatcatac cggatgaatc ttccactatg ctggtgactt cattattgct taaagaattc
    11161 aaatcgtttg gatcattacg cctgagtcca aatcccttat cattcagttt ggctttatta
    11221 agcttagccg cagaacccac ccataaaatg cctgccctgt ctatttcgag gctggtaaca
    11281 ttatctgtac ttaagctgcg gggatcgtcc gcgttgtgaa aatatcttgt aaataccgtg
    11341 cggttcttat tcaatttatt caatcctttg ctggtgcata tccataaatt accctgctga
    11401 tcttctttca tccctctgcc accccaggca tatgaactgt tattactcat tgacagcatg
    11461 tttttggggt catgttgata ccgtttaaaa cagccggtct tttgatctaa actatttaat
    11521 ccattattcg tggccaccca gatgataccg gccttatcct ccaggacaga ggtaaccata
    11581 ccgctcaggc tatctgcgtt aaatggatca ggacggtaat ttttaatgat ataccctgat
    11641 ggctgaccgg gtttcgctgc agttggtttt atttggtgta aaccggttgt attcccaacc
    11701 cataaggtac cttcccggtc tttataaata caattcaccc catcgaaagg ccagatggtt
    11761 ttattatccg gagacctggg ataatccaga tcaatagagt caggcaaaaa ttttcctgtt
    11821 ttcggattga agcgacacaa tcctgaggca tagcttccag tccaaatcat tccctcgttg
    11881 tcttcgttta taacacttat gtttggatca gaaaaccggg cattgggaga tggtttatac
    11941 cgcgtaaatt tttcagtggc ccgatcgaac ttacacatgc cttcgaaaga agaaacccag
    12001 atcacgccat gactgtcttc gaaaatagaa tatatccagt tttgagaaat agaattggta
    12061 tcaaatggat caaacctgta tttggtaaaa ttatacccgt catatttatt taaaccatcc
    12121 gaggtgccaa tccacaggta tcccttgctg tccagcaata tggctgttgt aatctcattt
    12181 gaaatacctt cttcaatgcc gatgtgttca aaacgcaaag tttcgttttg agcttggatg
    12241 ttttggtact cactaaagag aaaaatcaaa taaagtatac gcgtttccct gatgagccac
    12301 ctttgcatgc taattttaag cataatttga tgtcttaatt aaagtccaaa ttgggacgtc
    12361 ccatttcagt atcccggatt ttgcaccaag catgggtttt tatccaattc ggatttcggt
    12421 atcgggtaaa gcgttctgaa accttcggat ggtgtttttt ccggtattct ggcatcggta
    12481 taatgtccca gtcgtatcat gtcattccgg cgatggcctt cccaggataa ttctcttgca
    12541 cgttccgcta aaataccatc ggggttcatt tcagaaagtc caaaatcagg gagacctgcc
    12601 cggcttcgaa tggacaccct gtttattttt tgattaatgg tcattaaagc attctccaca
    12661 tccccgttcc ggaattgagc ttctgccttc atcagtatga tatcagcgag ccggtatacg
    12721 gcaaaatcat tactcatcag tccactgcca cttttattga attcccattt tgcgcagcgt
    12781 gctcccaaca atctggactc cggtgaagaa tttgaaaagt tggtgatgac gggatcaaaa
    12841 ataagaggga gatcaatagc gggatcgtat tggatatgtg cagaatcctt gatttgattg
    12901 acatactgtt ggccaaccag aaacattttt cgccggttgt cctggatgtc aaacagtttt
    12961 aaatattccg ctgtactgca aaatccgtta aatcccccag attcaagccc aaatgtagaa
    13021 taactgctat agtgcaaggt ggcaccctga atccaaaaag aattgagccc agcattgatg
    13081 tcgaaaggaa tcacaaaaat attttccccg gatttttcat tggctactaa aaaattagaa
    13141 aagaaatttt cctccagggt atatttattt gaagttagga tcgcattaca agctgcaata
    13201 caatcgttcc atctcggctt tcccgtataa acctcggcat tgaggtataa tttagcaaga
    13261 agtgcgtagg caaaccattg ggtgaccctt ccgtaggttt tggagttaac gtcaggactc
    13321 aattccatca aattatcctt aatttctttt tccacataag caaatacctc cggccggctt
    13381 ttattgccca attgcgaaca acctattgca tggatatcgt tcactatagg tacatttcaa
    13441 aacagatcaa gtgcgatata ataataaaat gcacgcactg tctttaattc agacagaatt
    13501 gcattcaaac ctgtaggctt gggatttaaa gtatttaccg tttctaaaat caaattcacc
    13561 cgtgctatac ccgagttaat aaactgccat gcctcggcca tatatgaatg gtccgatttc
    13621 cagctgtgtt tccacatttt ctcccaaact ccattatccg cccagtgtcc tccccggacg
    13681 gggacaatca cctcatcggt actggtttca tttaaaacat aggcggcgtt tgccggtgca
    13741 taatccctta agccactata agcaggagca acgccggctg ctatctggtc atcggtttgc
    13801 cagaacacgg ataccttgtc atatacttcg ggatccagtt tcaggcaagc ctggttgccc
    13861 caaagcatca tagccactgc caataatagg gcctggaagg gtttatacgt tttcataact
    13921 gtgtatttat atttattcat taaaatccgg cacgtatggc caatgaaaat cccctggtct
    13981 tgggaaaata atttctatca atataacgct gcgatccttc ggttttgatt tcaggatcaa
    14041 ttccgtcata gcgggtaaga ataaataggt ttgaggcgga taaatagact cttaagtttt
    14101 tgacactctt ccaattttta aaattataac ccagagtaat gttttccaga cgggcatagg
    14161 aaccatctct tacccaataa gtggatggtc gaggctgatc ggcaaaccca ttggtcaatg
    14221 ccttttcgcc aacattgatt gatggaaggt taacagcagc ttccaggttc attaaagcat
    14281 ttgcaaatat tttttggccc tgtaccccgc gtaaaaaaaa gctcaaatca aaatcaccat
    14341 aagtcaaatt attggttatt ccccaggtaa aatcaggagt tgggtcaatg aataccctat
    14401 cctgatccga atatgatgtt gaagtgccgg ctaatttgcc ttccggattg taattattaa
    14461 acaattcatg tccaccagta tccaatcccg catgtacagg aatccagaaa acacctgcgg
    14521 gataacctac ctgtaattgt gtaatataag ccccgcttag gccccctcct tccgcatatc
    14581 cataatggcg gttggtgatg gataagttgg ttcctttaaa tttaccggac aaattagtaa
    14641 tgttattctt taacgtagca aaatttgtca gtacttccca gttaaatctc tggtttttaa
    14701 gcaacccgat acccagagta atttctatgc ccctgtttat tgcagtggcc gcattggcag
    14761 atacctgatt ggtcaggaaa ggaggttggg ggaggtcata taagaataac agatcgctcg
    14821 tcttgtcatc aaaaaggtct atcgtgccac ttaccctgtc tttcaatata gagaaatcaa
    14881 ctccgaaatt gaacgatttt ctgacttccc attttaggtc cggattattt tcctgcatca
    14941 cggtataact ctgtaaaaac tgaccgtcgt acaaataaga acctgaacgt ccatacagga
    15001 gcctggaagc atagggtccc aggttttcct ggttccctgt aaacccatag ctaaggcgca
    15061 atttcaggtt atccagccag cgaagatctt tcagaaaatc ctcattggac gctctccagg
    15121 ctattgcaac tgaagggaaa tttccccagc ggtggttggg tccaaattta gaggacccgt
    15181 cccggcgaaa gcttgctgtg aggatatagc ggttattgaa ggtgtatata gccctaccca
    15241 gcaaagaaat cagcctgact tcatttttag cagaaaaaat atcggacgtt tggacatcag
    15301 tcgccgaact caggtcatta ttcagcaatt tagggaccag gaatcccctg gctgttacac
    15361 caaatgcgtc atttacaaac ctattgtatt catagactcc ggtgagatcc aggtgatgat
    15421 tgccaaaact tttatggtaa ttgccgtgga tatctccgga aaaaacctcc ttgttattat
    15481 tggattttac tgctgctgat tttccactga ttcccggaaa aacgggagaa aatttatcat
    15541 aaatatcgtt acctctggag aatgcgccca gaacacctgc cttaaatccc ttaaaaattt
    15601 catagtctgt ttttacagaa ccttgaaaat aattttcccg aaaatgacta tacacaccgg
    15661 ttaaaggata aagtggattt atggtgttaa cgttagccgg ctggtaaaaa gtaccatccg
    15721 gattataggc cggtagaaca gggagaaaat ccaagacacc gttaaaaaaa tcactacccc
    15781 gcagccggac ctggctggtc gtggattgat ccggcaggaa atttcgattg atcacagagg
    15841 cattgatccc atatcggact tccagccttt catttaaacc tttttggttg gctgtcaatc
    15901 gggcgttaat aacatttttc cccgtactga gtattacccc ttcctgagta atgtaaccca
    15961 gggagcctct gaaattaatc gcatcactcc ccccgctgat accaatggtg tgactttgtg
    16021 aaataccgga ttgagcaatt gccttttgcc agtccgtatt ccctccctta tccaatccac
    16081 cccctcctat cgctgccgta gcattcctcc attggtctgc actgagaaca ttcagttgat
    16141 tcgaaacacg ctctagccca acaaatccat tgtaatccaa tgaagttctt ccggtattgc
    16201 cttttttggt agtcaccaag ataactccgt tagctcccct tgatccataa atggcaccgg
    16261 cagaagcgtc tttcaaaata tcgtaggatt caatatccgc cggattcaat gtggccgccg
    16321 ccttatgaaa atcatcaata gccaccccgt ctatcaccag caggggcggt tgcccttcca
    16381 gggaagaagc acccctgatc cggacaataa aatctccatt gggatcccct cccggttgca
    16441 caatcaccat accggccact ttgccctgga gctgttgaag gggattggta atgacgcctg
    16501 tattaaatgc attgggcatt accctggtga ccgcaccggt aatatctttt cgctttgccg
    16561 taccataccc tacaatgatc aattcatcca gatttgtaat ggtttctgaa agtacaatgt
    16621 gcaattcgtt ctccgtccct aaaaccacct gataatcccg gtacccaaca taagtcacct
    16681 ccagcaaatc cccggaactt gcccggattg aaaaatgacc tacggagtcc gtgatcgcca
    16741 tggcctttgt tgccttgatg gaaacggtag cataaggcag cgcttctccc tcccggtcct
    16801 taaccgatcc tttcaggatg gattgagaat acaaatcccc ttccaggaaa aaaagaatct
    16861 cagaaaccaa aaccaatgac tggaaatatt ttgcaagacg ggacccgggc ctcataaaat
    16921 atcggattag aaaagtaatt taataaaata tttccaatcc gatgaggaca aaactactac
    16981 atcaaaatac cagttcctca tttccacgat acccctttta ccttggacca ataaggtctc
    17041 ccttaaagac ttcgttgcct aaggataact aaaagaatat cgttaaccag caggaatgag
    17101 taactggcgg taatgaatga gtatatggta ttggccgggg gcttgaagga ataagcctga
    17161 tgccctatgc cgctaatctc ttatcaaaat tgcctaaatg ggtttccgga agcgcttgaa
    17221 ttcttattaa gcattatttg caatcgaaat ttatatttac ctgttgtacg gatacctttc
    17281 cactttcact gatccggaca aaatagctgt ctttcatgac caaaacgggc ggcatcccct
    17341 ccttcggtcc ggtcaagtca tttaaaatgg ttttcctgtt ttccgcctga ttaaaactga
    17401 tgacctgcgt tgaacttgat tcttcataaa tattaccggt ggtttccccc acccctttta
    17461 tatccagggc ctgataatag agattggccg tgaaggactg aggtgtgtga aaagtcacaa
    17521 cggcattgag cgatagattt ccggaaacct tcacattttc ggattttcct ccattgctgc
    17581 agggcacaca ggtgaccagg tcgaccggca ggatgtaacg ctgattggtt accatcggga
    17641 acgcgctggt ttgagctatt tcttcttccg gcagggtaaa ggtatcctca caagccaaaa
    17701 tggaaagaag aaggaaaata agcattaggt ttttcatgat agcatggttt aaaatggatt
    17761 aaaaaatggt ttctcttaaa tttttataat taaaattaga ccccggtgaa gtgcagaggg
    17821 taacgggatc caacagcagg tgagttaaaa tgagcaaatg gccctttaag cgggtaattg
    17881 gattttaaat gttaaaacac cctggggata accttagtcc gaattttact attgtttaag
    17941 gaaataattg aaccagggag atccgttcca gtaactttac ggttataaaa aaccaaaact
    18001 catgaaaaat attctttttg cctgtgcttt tattttagtg gcagggaccg gcttcagcca
    18061 gacgcccatc gacacctata tcaaagaagc ccaggctttt atcgcacaga aaaattacaa
    18121 acaggcccaa ctcagcctcc aggacgccat caacgagata aataatatcc tggccaaaca
    18181 gatcgccgag gccctgcccg ccgagatcaa tggcctgcaa gcaaccggag aaagtgaagt
    18241 caacagcgcc ggatcggtca tgggcatggg catgcagatc tcccgttctt accaacatcc
    18301 ctcaaaacct gaaaacagtg ccgaagtcat gatcatggca aactccccaa tgctaaacgc
    18361 cctgaatatg tatatcacca atccctccct catgggccag ggggccaaaa gcgtgagggt
    18421 gggcaccaaa agagccgtcc tgaaaagcga aatggaagaa ttcaccgatg ataaaggggc
    18481 cagcaaacag atccgggtca ctgaaatcca ggtacctatc agtcaaaccc tgtttaatat
    18541 caagaccagg ggatttgcaa ccgaacagga agaactggct tttgccgcta aactggagat
    18601 ggataaaatc aaggatttga tgggggagtg atcagacgtc aagagtcaag agtcaatagt
    18661 taatagtcaa tagtcagtta acaaccggga agggaaataa gccggcaacc tgggcattcc
    18721 gttgattgcc accggtggcc ggcaactggt tataaaagta attatcccgt caccgctatt
    18781 ggaattgcgg taaaaggaca aaaactgacg atcgccgaaa atagatgcag gttttggccg
    18841 gatcaaacgg caatgagtaa gtggctactc cgggtgattg gattccggct cggaccaggc
    18901 atcaggatac aaccttcttg cccatggatt gctgctggtg gtacagcagt ccggcatccc
    18961 agatccgggc ggctacctga aattaaattg aagatcaccg gttttgtcgc cgatggagac
    19021 ggcctccctg aatgcaaccg gtgaaaaagg gctgtcccac agaggtccct gcggaaaaag
    19081 accttatcct ggatttcttt tggtcaaaca ggaagttgta ttataaattc agtaccccgt
    19141 ccttcctcgc tttttacttc aatgcttccg ccgtgtgctt taatgatatc ataagtgatg
    19201 gacaatccca aaccggttcc ttgcccggtg ggtttggtag tgaaaaaggg ttgaaagatc
    19261 ttatcgacca tttgtggtgg aatgccgttt ccattatcag cgatatggat gaacaatctg
    19321 tcagctcccc ttctggtccg gacaaccacc ttgggttcaa aattttcaag gttcgtattt
    19381 cgtttttcgc ttacggcata aaaggcgtta ttgtacaaat tcagcaatac ccttcccagg
    19441 tcttgtgcca cgatgtttat tttgccggct gagggatcaa aatcggtttc caggaaggca
    19501 ttgaatcctt tatcctttgc ccggagcccg tgataactca atctaaggaa ttcctcacac
    19561 aacggattga tgtcggtcag ttccttctgc cccgcatctg accgggaatg ctgtaacatc
    19621 cctttgacaa tggcatccgc cctctttccg tgatggatga ttttcaattc gttttcttcg
    19681 atgcttttga gcagttcctc ttccaacgct gtgctccgtt cgcctttggc ctttaacctt
    19741 tctcctctca attcctcaat caattcctta ttgacctcag agaaattatt gacgaaattc
    19801 agagggttct ggatttcatg agctatgccc gcagtaagtt ctcccaggga agccattttt
    19861 tccgaatgga tgagctggtc ctgggtggct ttgaggtttt ccagggattg ggccaggtct
    19921 gcggtccgtt ctttcacttt ttgttccagc tccaaattct gtttcgcaat cagggcaagg
    19981 cgttccgctt ctttctcatg ctccagcttt tctttggcca gcagggcttc tgtgatttca
    20041 ttctgctggc tggcatacct ccggtagaga taccaggaaa acatcaccac cagccaggca
    20101 atcccaagcc ccagtccgta gttgaacagg gcatcggctt gtccgtaggc tttcaattgc
    20161 tgatcaaaca gcgcgtagat gatcagggca gccaaaaaat acagaatgaa gggaagggcc
    20221 gcggtgataa atggccttct ggccggtcct tttaccagca ggcatttgac gatggtcacc
    20281 ccaagcaaaa ggatatacag gatcaggaac agacctttaa aaatgatgac ctcgggccgg
    20341 tagaagaaat cgacgggcac cgggggtacg accaggttga tcaaataaat gaccgacagg
    20401 tataccataa accggtccca ttttggaaac cgttcaaagg acctgaaata gtgtcggata
    20461 aaatgcaata cgaaaaacaa ccaggaaagt ccgagccggt ttatgaagtc ggaggtcagc
    20521 ggatattccc tataaaggat atccgtaaac aagggattgt accagaagct gatggacagc
    20581 aaaaagaggg aaaagtagag gtacagcttt tccttcgccg caaggaagat cagtaaattg
    20641 agaattgcgg ccagtataaa aaatccggcc aggaaggctg gaataaagat gtcctcccgt
    20701 acatacccgg attcataatg ctccagttcc gattgcctca gtttttcggt atggtacccg
    20761 cggagggtca ggctgtccgg aaggtcgctg aaaaccgaat tgatcttatg ccagcggaaa
    20821 taaacccgga gggtctccat cgagtccagg ctaaggggga cggcactcac cctcttaaaa
    20881 ccgtctttgt ttttccagga ataaagattg ccggtgacca ggtggatcat gccggcaccc
    20941 ggggtcgaat ccctgaatat gtaaaaatca gcccgggaag cctgggaact caggctgatc
    21001 cctatccttt tatccaaacc gtttttgatc cggaaccgga accagcaggt atgaatgcct
    21061 tcttccattt tccggtcccg gaaatgataa aaggaatccg ccaacaaggg ccccgacacc
    21121 tgattgatat cgagggtgcc cggtttgtct tccagaatct gccagtaggc cgaatccaga
    21181 tcgtacctca cggcggtgtc ggaagtgatc tggtaaaccg gaggccagga aacctgggct
    21241 ttcagaaaaa agacgaccag gataaaccaa ggcgtgagca attttttctt acacatttcc
    21301 ggcttaaaag tattgcattt tgacaggaat ttgaatccgg cccgggaatc aggcttggtt
    21361 ttcaggatca tgggagtttt ccgccctctt ttatcaagga aaaccgtaat tttgcatcgc
    21421 cggatcatta aaaatcgact ttcccttttt attagatcag ctgtccgcct tcgggctgtc
    21481 aaatattttt tcattcaacc ccataacatg gcagaatcct ggaataaaaa agaaagagaa
    21541 aagaaaaaaa gagcaaagaa aaaagaaaaa gaggaacgca ggcttgagcg gaaggaaaat
    21601 gcaaaggact ccaaccccaa tgattatttt tcctatgtgg accatgacgg gaatgtgacc
    21661 agcactcccc ccgatcctgc gcagaaaaaa gtatttaagg cagaagacat cgaaatcggg
    21721 gttcccaagc aggccgaacc tgatccggag gaaaggatcc ataaaggagt ggttactttt
    21781 ttcaacgaat ccaaaggtta tggttttatc cgcgacctga agagccagga aagcatattt
    21841 gtgcacatta acgatctgga ggaagccgtc cgggaaaaca gcaaggtgga atttgaggtg
    21901 caaaaagggc cgaaaggatt ccaggctacg caggtgaagg tggttagata gttagccagt
    21961 tagatagtta gcaagttagt aagttagaaa gttggcaagg tggggggtag gtttctggcc
    22021 taatactctt ccatccccct catgaattcc agagtcattc caggggcaat tcaataataa
    22081 attcagctcc ctggcccggc cttgaaaccg ctttcaaatt tcccgaatgt cctttggtaa
    22141 tgatatcgta tgccagcgag aggcccagcc cggtaccttc tccggtaggt ttggtggtaa
    22201 aaaagggttg aaagattttt tcgaggatgg cttcgggaat accgtctcca ttatcctcga
    22261 cccgtacttc cactccattt ttcagtttat tcgtggttac ccggaccaaa ggctggtaac
    22321 ccgaatcccc cttcttagat tttttatata cttcgtaaaa cgcattatta aagagattaa
    22381 gcaggacccg gcccaggtct tgcggtacgg cgtggaccag gggcagtgcc ggatgggcaa
    22441 ccagttcaaa ggccgaatta aaactttttt ccttagcccg gaggccatga taagacaggc
    22501 gcagaaattc atcacttaag gtattgaggt cggtgggttc tttttgcccg ctgtgtaccc
    22561 tggaatgttc cagcatggac ttgactatgg tatcggcccg tttcccatgt tgaaggattt
    22621 tttcctggtt tgaccggatg gattccagta tctctgattt aacgggtacc cattccactg
    22681 ggtttccatg ttccggcctg ttttcctgcc ctttttcttc tctattcaaa ttctcctgaa
    22741 tctcctgaaa caactcctgg ttgatttcag caaaattgtt gacaaaattc agcgggttct
    22801 gaatttcatg ggcgatgcct gccgtcaact ctcccagcga agccattttt tctgcctgga
    22861 tcaactgggc ctgggtcgct ttcagttcgg aaagggtttt ttcgagggct gcattggttt
    22921 tttgtttttg cagattgttg cggtacagga cgaaggcgac caggagcaag gctcccaggc
    22981 caatcgtcaa tccgaaagtc cggtttctgt ttctcaattc ctctttttcc ttctccaggg
    23041 cctgcagccg caattgttca tcgaatccga tattctggaa ttgctggttt tgcttgatat
    23101 taaaaatgtc ttcgttcagc cggatgatca ggccctgata ttttaccagg ctgtccgtga
    23161 agggagtagc ctggtaataa ttggccaggg cctggtaagt ccgcaacaag aggtctgggc
    23221 catccatttg catggccatg tccagggcct ggttgatatg aagcatcgcg gagtcccgtt
    23281 tctgcgcctt gatgaaatac tcggagagaa ggagattggt cgccaccact ccccggaaat
    23341 aattttgatc caggctggca tccagggcct tccggtaaaa cccggctgcc agttcggcat
    23401 tgccattgta ttggtgtatg cgtccggtgt ttaaataaat gctgcccaaa tacctgggaa
    23461 agttcatccg gagagcctca tccagggcgg tttgttccgc aaaaagagcc gagtccggct
    23521 tattcaggac caggaaaagc ctgcccatga tgaggtggat cacgcagagc agccgttgat
    23581 tttgggttac attgcccagt tcgagggctt tgtatgccga ttccagggct ttctcaggat
    23641 tatgggtatt gctgtacagg atggccaggt actgatgcag gtgggacaat ttgtccaggc
    23701 gttgaatgcg ggccggcatc gaccggctgg tgatctcatc cggcgccgga tattgggggc
    23761 cgagcacatt tttttcactt gcggggtcct cagccagggc aatggcggat aaataggttt
    23821 gcaacgaacg ggcataattt cccttgttct gcagggcata acctattttg cccatggcat
    23881 aaatttcttc cagcctgaga ctgagtttga tagccagttg ccggatcagc tcggcataaa
    23941 acaaggcgct atccgggttg acctccgtaa acgcatcctc cagcaaaaaa gccagggaca
    24001 atctggaagt gtcgttatcg gttgaccgga gttcctgcct gaggctgtcg atattcggtc
    24061 gttgaccctg gctgatccgg gcagcaaaaa ccaggaccag ggcaaccacg aacttcaatg
    24121 ccaaacccgg tttcatattt gtttttatgg aatggaaaag ctaatttcca tcgctggtga
    24181 aagtcaagat tatgtttacg aaattacaaa ttatatccct cctgttaggt ctggctcttt
    24241 acccggcctg caataaaaac gtacaagtta aaaaaacggg aaaagaaccc gccttccggg
    24301 tcctgggtta tttgccggct tccagggact ggctggctgg cctggagcaa atcgacctgt
    24361 ctaaaatcac cgacctgaat cttgccttta tcaatcccga cagttccggc ctgttcagag
    24421 agaatgagga ttaccttgaa atcgtaagga gggcccatgc ccggcgtgta cgggttttta
    24481 tatccatagg cggaggcagc ccgctggcct acctcgaaac cctcatggag aaagagcaca
    24541 ggaccacaat gatttccagt ctgaccggaa tggcggtaaa atataaattc gatggcgtgg
    24601 atgtcgatct cgaaaacgat cttattaatg ccaattatgc tcccttcgtc cgtgaattgt
    24661 cctcctctct taagaggaac aatctattga tgactgccgc gctggccagt tggaatggcc
    24721 atctgatcgc cgactccacc ctgcaacttt atgattttat caatatcatg tcctacgaca
    24781 aaaccggtcc ctggaatctg tccaggcccg gcccgcattc tccttattcc atggtggagc
    24841 aggactttgt ttattataat caaacgcgga aaatacccgc acaaaaattg ctgatcggac
    24901 tgccctttta cggatatggt ttcgggcccg gagtgcctca aagcaagtcg taccaggaaa
    24961 tcgtcaatct atatcccggc gcggaaagca ccgactcgat caccgtaccc gagggaggta
    25021 aagtgtatta taacggtaag gccactatcc gtgataaggt tttgttcgct caccggaacg
    25081 gagctcgcgg ggtgatgatc tggcagttat tgggagacag tcgggattcc cgctctttgc
    25141 tgcaagtgat ccagggagct atttcgaggt aaagcgatcg aatagagcgt agacaccctg
    25201 agttttgcgc acggagggaa ggagagcacg gagcattcat tatacgatca tcaaattgag
    25261 cgcctatcaa tccagcgccg gcgggagcag ttttaaatct tcaaataacc gagaccggct
    25321 cccgcaagct agggattgtc ttacatccat caaatcacta atatatctcc gtggcctccg
    25381 tgatctctgt gagaaccatc tccgtgcgat cccagagttt tgcgcacgga gagaaggaga
    25441 gcaggggcat ttcttattac gatcatcaaa tcaattaaaa ttaatctcca tgtcccccgt
    25501 gatctccgtg cgatccctga gttttgcaca cggagagaag gagagcaggg gcatttctta
    25561 ttacgatcat caaatcaatt aaaattaatc tccgtgtcct ccgtgatctc cgtgcgatcc
    25621 ctgagttttg cgcacggagg gaaggagagc acggagcatt cttattataa tcatcaaatc
    25681 tttaatatta tctccgtggc ctccgtgatc tccgtgcgaa ccatctccgt gtgaaccacg
    25741 ctgcgatcga cacctgctca taacctccat tcctactccg cccccagtct cctcaccgcc
    25801 agataagcca tcagcccggt gctgatttcc agggcttctt catcaatatc gaaagagggg
    25861 gtatgaaggg aggaaatgat gcctttggac ggattgccca ctccgaggag ataaaaacag
    25921 gcgtccgtgg cctgggaata ataggcaaaa tcttcagcgg ccatccagag atccatgtcc
    25981 acgaccttgt ctcttccgag atagtcttcg gtaaaagtcc tgagttggga agtcagtttt
    26041 tcttcattga ccaggaaggg atagccccgc gtgatctcca tctcgcaagt tccgcccata
    26101 cttacagcga ggtgttccgc cattttggtc atccggtgat gcgcgtccgc cctccatttt
    26161 tcatcgaaag tcctgaaggt gccctctaca taaacctcat ccggtatgac gttgatcgcg
    26221 ccgttggcga tcaccttgcc aaaggaaagt acggtgggga tggaggggtt ggccatgcgg
    26281 ctgacaattt gctgcagggc gaccaggata tgggcggtga taatgaccgg gtcaatgttc
    26341 tggtgcggtt gtgcaccatg gcctcccttt cctccaatgc gcatgatgat ctggtccatg
    26401 gacgccatgt gtttacctgc gcggaacccg atctttccgc attccaggga gggcatcaca
    26461 tgctggccaa tcacggcccg gggttttgga ttttccagca caccgtcccg gatcatcatg
    26521 ctggccccgc cgggcagttt ttcttcgccg ggttgaaaga tcaattttac ggttccgctg
    26581 aattttttac ggacggattg caggatttga gccgtaccca ggagggagga agtgtgcgca
    26641 tcatgcccgc aggcatgcat cacacccggg ttttgggata taaaatcaaa agtgttggtc
    26701 tcggtgatgg gcagcgcatc catatccgcg cgcaaagcaa ccacacggtc cgggccgccg
    26761 tcgcctggcg attccccgcg gatcatcccc actactcctg ttccggccat agcctcccag
    26821 ggaattccca attcatctaa ccgggctttg acataaccgg atgtttccct ttccaaaaaa
    26881 gagagctcag gatgggcatg gagatgccga cggtaaagca cgacgttttt gtggattttt
    26941 ttagcctccc ttttaatatc gttaacgagc ataagatgga aatggctgta aggtaagggg
    27001 aatggagaat gaagacaaaa ggaatgggca gttttgccca attccgtggg tgtgtcccac
    27061 gatggtcaat cgctgacaat catacgctcc tttatcccgc aaccggcaaa tgaattacaa
    27121 attccgttcc ttccccttct ttggattcaa cagtaatgct tccccgatgc gccttgatga
    27181 tatcataagc cagggataat cccaggcccg tcccttgccc ggccggcttc gtactgaaaa
    27241 aaggctgaaa gattttatca atgatgtttt gaggcatgcc ggaaccgtta tcccggatgt
    27301 ggatttcaat ttcctcaccc cgctttttgg ttgagagggt gatcgtgggg gagtatgccg
    27361 ccgagccagg gggggcctca gagccacccc ttgactttgt tgcacaagcc caaaacgcat
    27421 tattggacaa attcaataat accctcccga tatcctgggg tatcacattt accagtggaa
    27481 gatccggatc caggtccagc ctcaattctg ccgagaaatt ctggtctttg gcccgatacc
    27541 cgtgataagc cagtttaaaa tattcctctg caagggaatt gattgcagtg ggttgtttgg
    27601 ttcctgaact ccgctgactg tgcaacagca tggcttttac aatggcgtct gcgcgcttgc
    27661 cgtgctggag tattttgtcc tgattttctt ttagggtggt caggatgtat gattcggcac
    27721 gctcatctct attctccttt tcctttctcc tttctccttc catctcctcc agcaactcct
    27781 tattgatctc ggcgaaatta ttgacgaagt tcaaggggtt ctggatttca tgggcaatgc
    27841 ccgcggtgag ttcacccaat gaagccatct tttccgattg aatgagctga gcctgggtgg
    27901 acttgaggtg gtccagggcg gtttctattt ccttattttg agcggcaagc aatgaattgt
    27961 ttttttttcg ctgcaggttg cttcgccaca ataagactgc gacgataagg atggtgctta
    28021 acccaccgag cagggaattg gtttgaagcc ggttttgata ttccttccgg gtagcctcta
    28081 cttcctgctg tcgttgttgg gcatctaaat caatattctg aaattgctgg agtagtttcg
    28141 taccaaaaag actttcatta attttaataa tcaaagactg gtattttacc gtgctgtcgc
    28201 tttggccagt cagtttataa tagtcagcca tggccgtgta acaccgcaaa aacaggtcgg
    28261 gggcatttaa cgtttgcgtg acggtcaagc cctgcgtgat atgatattta atggaatcgg
    28321 cttggccgga ggatttaaat aaatccgcca gggcgaggtg actggcgacc acccctctga
    28381 aatagccgtg atctgaactc tccgacatgg acttcaggaa ataagttttg gccaattccg
    28441 gctgttttca tcgcaaggta aaccctgccg agatttaaca ggttacttcc caggtatcgg
    28501 tgatagccgg ccctgatcgc ataatcgtac cccttttgaa gggaggataa agctgagtcc
    28561 gggttattcg atgccaggta tattctgccc aaggtggtat gggtgatgct taataacctt
    28621 aaattattgg actgctccgc cagggagatg gcctctttaa aataggtcac cgctttttca
    28681 taatttcccg tattgccata taaaataccg gtgaattgaa gaattctggc caacttggtc
    28741 agccgctgat cggccgggga tacggtacga tccatgaagt catcgttagg tggaaaacgg
    28801 gagggtaaaa ttattttctc actggcagga tcttttgcaa tggcaattcc tgataataag
    28861 cttcgtaacg accggggata attgcccagg tttagctgtg cataacccat ctcccccagt
    28921 ccgacgactt cttccagtct gagattaagt tttctggcca gttcagggac ctccgaggca
    28981 tagtgatagg cgctgtccgg attgatttca gaatattcat tggcgatcct ttccagaagg
    29041 agcaaccgaa gggtatcgtt attttccctg ctgatttcgt atttcaggct gtgaaaaatg
    29101 gcaggttggg ctgaaacagc atttatgggg agcaggagaa acaggaaaac tattcttgcc
    29161 aatgtatttt attttgctat taatgtaaaa tgaatttcgg atattccttc ccggtattcc
    29221 acccatcact caacgctatg agcacaggct aaagataacc caagaccagc agccttgtcc
    29281 gcaggcttag cagaaaaacg gtttaaagat ttatcaaata tatgtttagt cattcccggc
    29341 ttggccattt ttaatggatt acaggcaatc ttaagttaaa ctccgaatac tctccttccc
    29401 gggattcaac ccataactcc cctttatggg ctttgacgat atcataagcc aaagacaatc
    29461 caaggcctgt gccctggccg cttggtttag tggtaaaaaa cggctggaat attttatcta
    29521 ttaggcttgg tggaatacct aagccgttgt cctttacctg gaggataacc tgatttcctt
    29581 cttttctggt gcggagcaaa acggctggtt catattggtc cggttgccgt cgtttctttt
    29641 ctttcaccgc gtcgaaggca ttggtaagca gattcaggat gaccctccct atgtcctgag
    29701 gcactattga aattttctga aggctcgggt caaattcggt tttaagctcc acgttgaagg
    29761 atttgtcctt ggcgcggtat ccatgataac agatccggag atattcatcc gccaggccat
    29821 tgaggtccgt ggcttccgcc tcgcttttcc cggtgcggct gtgttgcagc atgcctttga
    29881 cgatggcgtc ggcccttttg ccgtgggtaa taatctttaa ttcgttttcc cgtatttcat
    29941 ttaacaggtt acggacgccc gggacatttc ctttattcat ttcctgttcc gcctcatcaa
    30001 tcaattcccg gttgacgtct gaaaaattat tcacgaaatt caaggggttc tggatttcgt
    30061 gggcgatgcc cgcggtgagt tcgccgagag aggccatttt ctcggattgg atgagttggg
    30121 attgggtggt ttggagatcg ttcagggtct ttgccaggat ttggttggcc ttttgttttt
    30181 gcatattgtt cctgtataag acgataccca accctgagaa aaccaggacc agggccaaaa
    30241 gactgtatat tttgaccctg ttcttgaaat tcaatctttc cgcctcgctt ttttgctgct
    30301 tttgaaactc attaaaagac acccgttgaa gttccctgat tttctgagga ccaaacaaac
    30361 tgtccttcac ggcaatactt aatttttgaa aataataggc gctgtccatc cggccaccgt
    30421 caaaatgaag ccgggacaat aagttataaa ggtccaggct gatgtttctt tgttccaggg
    30481 attgactgat ggataaagcc aaccgggcac aatgcaaact cgaatcggct tgattcaaag
    30541 cattgaaggc ctcggccatt ctgtacaggg cgccgctttg gtttctcatg tcgccggttc
    30601 gttgtgccct gtttaaggct tctctcccat aggccagggc ctccccgggc tggcccatca
    30661 atgattttaa ttgcccaagc ctggtaagga tcaaattctc cagggcaata tgataagtct
    30721 gcttcagata ctcccgcgcc ttcaattgaa aaaataaagc ggaatccagc aaacccattt
    30781 tttcataggt aaatccgata ttggacaaat caaaacttcc attcaccggc atgttgagac
    30841 ggtcatgaag aacaaaggcc tggtttaaaa aatccaggcc cggtggatag tcccctattt
    30901 ccgtgtacgt caatccaata taggtcaggc tgtttgcttc cgcaagggca tttttgaggc
    30961 tcctgctcat ttccagggcc ctgagctgta tttccagcga tctggtatat tccccgttga
    31021 cccgtgccgt ttcacccaac aggttcatga cctgtacctc acccttttta aaattcaatt
    31081 taaccgccag gtcctgggcc tcccgcatca ggtccaccgc ttcctgcggg cgtgcaaaac
    31141 ggtacaggtc cgccaattga atgagcgtca gcacccggtt ggtgtcctgc ccggatttgg
    31201 acaaatattg aaacaggctg tcttcccggt tggtttggct taaggctatc caggccaata
    31261 aaagtaaact caacataaaa acccaggacc acggattatt tttatacaat gcaccgctgg
    31321 ctggcaaact ctttcccatg attatccaat gtttatcccc ttttaaacgc tcttgttcca
    31381 ggccagatcc cattttttag attaactttt cggtaaactt atgatgaaag atgatccttc
    31441 cccttcattc gtttctaccc gcaggtcccc cccatgagcc tttatgatat cgtaactcaa
    31501 actcagtccc agcccggtac cctgacccgt aggttttgtc gtaaaaaaag gctggaatat
    31561 tttatccaga atattttgcg gtattcccat gccgttgtct atcactctca actccatgcg
    31621 gtcattgatt ttttgggttt ttacagttac ggtaggtttg tattccaagc catctgtgta
    31681 ctctgaggtg ccgctttgat tttgtttttc agtgaccgcc cagaaagcgt tgttgatcag
    31741 gttatggagg actcgcccca tatcctgggg gaccacattg aatttttcaa gatccggatc
    31801 caggtccagg acaaaggcgg ccttgaatgt catgtccttg gcccgcagcc cagcctggaa
    31861 ggcgtgatgg gccaggcgca ggtattcgcc ggccagggcg ttgatatcgg tgggttcctt
    31921 tttgccttta ctggctgggt caacactccg ggagtgttgc agcatgcttt tgactatact
    31981 gtccgccctt tttccgtgct gaaggatttt cgcctggttt ttacggatgg tatttagaat
    32041 ttccgtttcg atgagttcag atttttcctc ccccgcttcc ggccttctga acccggaatt
    32101 tcttttccta tgttcctgca tttcatccag caattcctga ttgatttcga taaaattagt
    32161 aatgaaattc aaggggtttt gaatttcatg tgcgatacct gcagtgagtt cacccagtga
    32221 agccattttt tcggactgga taagttgggc ctgggtggac ttcagttctg ataaagtctc
    32281 ttctacattc cgtttttgaa tttctatttc atctttttga ttcttcaaca aaatatttgt
    32341 tgcctttctc tgtctgatat ttctggaaag caaacctgca attaacaata agaccaacag
    32401 tcctcccgcc aaagaatata tccatgcctt agcttgtaat tttgcatttt gtgctgccat
    32461 ttcctgctga tgcaattgtt cattaaaaac cagcgtatga atttgttttt ccttttccct
    32521 tgtgaagata ctgtcattgg tcattattgc aaccttttga tagtagaaag cactgtcaaa
    32581 cccatgccgg ctttcaaata atttcgacaa aagggcattt gcatttacta tagcagaagg
    32641 gttttttacc cggttagcga tcgttaacga taatctcgca taatgaaagg ctgagtctga
    32701 ctgaccggct tcaaaaaacg tttgggcaag ccgatcataa acttcagtca ggctgaaaga
    32761 ggaaatactt gatttcgatt ccaggtccac tgcttgccga taatgagtca ttgccaggtc
    32821 ttgatttcgt ccttcccggt ctatatcacc aagcaagaga aaagcacccg aagcagggct
    32881 taattcaagg ttgaggtttg catagtaact cgctgaatcc aacttcccgg tatggtaata
    32941 gaccctggca agatttaaaa acaggccgct ccgccaaaag gccctgatgt cttccgccga
    33001 cgaagtatat ttaatcgctt tcaggtaata atatttagct ttatcatatt ctgctatggt
    33061 tccaaaaata tctccaatgc tttgattcac caggactaat tgaaatgaat cttgttgaag
    33121 ttcagctatc ggcaatactt gcgaaagcaa ttccagggcc ttgactgaat tgccctgttc
    33181 cttaaatacc gcccccagtg aaatcatgga ttggatttca gcatttttat ccttgctcct
    33241 tcttgaaagt tccaggctgt gcagtgcata cttataactg ctgtctaaat ggatctcccg
    33301 ataataataa cttttcctca atccgaccag aatagccgtt gaatcagcca gcccagcaag
    33361 attgatataa tcatccatcc atccttcaat ccgtaactgc accgtggtgt cctgtttaat
    33421 ttcaattacc cggtcttcta tgtcttcact gaaggccgtg gtttccacat tattccagtt
    33481 gccccgggtg aatttgaagt aatactttcc caaaggcata tttttgagga cgatatattt
    33541 tcggaaggaa tcaagcgtga agagtctata gttgtggtcg cccggattcc agttattaaa
    33601 gccccctgct aaaaaaatat ccggtttccc ggcgggatcg tacacctcaa gccgaatgga
    33661 tgatcttgcc gaatcagagg ttttgtgcgc atcataaaaa tcatcgatcc aacccgaaat
    33721 atgcgctggt attaccgtat ccttggttat ttcgattatc ctgttcgcta tgcttttacc
    33781 gctcgaagaa gtctctacgg tttcccaatt gccccgggta aatttaaatg aatgccttcc
    33841 tgaaggaatg tttttgagaa taatatacct atgagtggca ctaagggcat tcagcttaaa
    33901 ggcgtggtct ccaggattcc agttattgaa gctgccggct aaaaatattt cacctttttc
    33961 ataagggtcg aacacatcaa atcgaacggt aaattgtgca gacagaggga aaaggtgcga
    34021 caaaaataat agaatcaaac ccgggtttat taaacccacg atttgagttt tatatatatt
    34081 aattcccttt ttaattatca tcccattcca attctcattg gatttaaatt tgtcacgcga
    34141 ttgagcataa atagaagtgg atcgtaaatt cagcctgatt caaatctggt actttcaatc
    34201 cccttccatg agcttaccaa tatcctaatc caaagaccac cctaatccag tccactgccc
    34261 ggtcgtctcg gtagccatcg ggataatgta aaaaagggtt gttccaaagg ttccccagca
    34321 tgaggcgatt ttaacatttt tgcactcatg ttgaaggtaa caaaatttga aaatctgatc
    34381 cttccggagt actgtttaaa tgaatatttc caccgtgggc tttaacgata tcataagcta
    34441 aactcaatcc caatccggtt ccttgtcctg ctggttttgt ggtaaaaaaa ggctggaaga
    34501 tcttatccct cacctcttca ggtatgcccg ggccgttatc tttgatccgg atctcaacgg
    34561 tctggcccgt tcttttggta atcagggaaa cctgaggcgt aaacggttcg cctcttgact
    34621 tcatccgttt ttccttatcc tgaactgcgt aaaaagcatt attgatcagg ttcagtattg
    34681 cccgggcaag gtcctggccg gacacttcga tcaaacccac cgtatcatca aaatcagtat
    34741 gcagcgcggc attgaaggat ttgtcttttg ccctcagtcc gtgataggcc agcctcaggt
    34801 attcttcggc cagcttattt aggtcggtgg gttccttttt gtttttaccg cccgcatcac
    34861 cgctgcgcga gtgctgcagc atgcttttga cgataccgtc cgcccttttc ccgtgaaaat
    34921 tgatttttat caggttttgt ttgaggtctt cggtaagatg caggacctcg gctgaattcc
    34981 ccgcctgaat ggcctgtctg atttcctcga tcaattcggc attgacttcc gcaaaattat
    35041 tgacgaaatt caagggattt tgaatttcat gcgcaatgcc tgccgtgagt tctcccagcg
    35101 aggccatttt ctccgactgg accaattgct gctgggcttg ttttagcccg gcaagggcat
    35161 tttcgacatc cttcttagcc atttccaatt gacggaaatc gtcatagcgt gcataagcga
    35221 ccgaaaaggc atcggagagc gattgcacga ggtccagctc cgtatccgac aagggcgcgg
    35281 tgttgcctac atacagcata ccctgcctaa aaggcgacaa atgcaggtaa atgccatccg
    35341 ccggaacatt gcttaaatac tcatcccgtg aatcaaccgc ccccaagtgt gccaataaat
    35401 cggcctgagc ctggaaatcc gtatccaccc accgggtcac gtacgcctta tgttcccgcc
    35461 aatactgaac cgcctcgcca agatttcccg gattgtccaa aggcaaatga aaggcggcaa
    35521 tggcccggcc atcgggtgtg gataaatagg tatgaatttg gttgttgggc tcatccatga
    35581 taaagactcc gcagcgcacg aatggaatac ccagcgtggt gagttctttc catatcaatg
    35641 gcgtgatccg gtcaagatcg gctgccgtac gcatgctggc gatctccgcc cttaagcggt
    35701 ccagcgaagc ctgccgcacc gcttcttttg cacgggcttc ggcattgcgc aaatcgcggt
    35761 aacgtttgta ggtgagactg agcacggaag taaacctgcg gtaaattttt aactcatctt
    35821 cagccaatgg cttttccgtc caggcgtaga ctccgccttc atcaaagaaa aagaaatgcc
    35881 cgtattgtac ggcatcctgc gggtattcgg gaaattgaat ttgtggccgc aataactgat
    35941 aatactccct gatttcattt cccctcaata ccggatgata ttcctcctgt gccagccaat
    36001 gctcataaac cccctccagt acgaaatgat tttcgaggac cagttttccg agtatttcaa
    36061 tggactcgcc atccgatgtg gtgttcatcg tggacaattc agacatccgg gtttcccggt
    36121 ctatcaaacc cacgccacac cttcgagggg taaggttcag cgaattcagt tcccggtaaa
    36181 aggtctggat ggtagcggcc aggtcctgtg agctgtgcat ggccatagct ttactcctga
    36241 ccctctcgag cgaagcttcg atcctggctt ccctggtctg ggcttcggcc ctttgcaggt
    36301 caagaaagcg cgtataggct tgttcaaaaa cccgtgaaaa tcttcgaagg gtattcagat
    36361 cggcttcagc aaatagatgc cctgaccaac tatccaaacc cagcatggta tttttctctg
    36421 cgacaaagga aaaattccaa gcaggtaatt ctaactgaag ttgtttcaat tgatcaggta
    36481 ttttagaacc gttgtgccgg tgcacatatc ggaaatagtc atttttggcc tcaaccgagt
    36541 atacccggtt tagcagttgg cctccaattt caatggtatt ccaataatca agaattactt
    36601 gattgtcttt catgacctcc tcgtggggaa gatctatgaa gaccggtttg gttaattgga
    36661 cggtagcgat ccaaagtctg atgtttttgt tctcccggtt gaacacaaac agcaacgccc
    36721 catccattgc aatgccaagc cctaccactg cttcaaataa agatccagct acctgttcca
    36781 gttcatccga gcggtgcatg gccaaagatc gtgaccgcac cctttccaat gccgattcta
    36841 tctgcgcttc tcgcgcctgg gcttcggctt tttgcaggtc aaggaagcgc gtataggttt
    36901 gttcgaagac tttggcaaag cgtttgaaaa tatcgtgcgc ttcaggtacc tgctggtaag
    36961 tgataaaaag taaaaatcct tttgaaaaat aagcacaatg atcgacctca taggtgggaa
    37021 gtggatgacc ttctgccagg atagattcca attgttcgcc gactacagga agggtgagca
    37081 ggtatttgta attctcttca agtgatttac cactaatttc gtctatatac aattccaacc
    37141 cttcattatg cgcttcgaga taacggatga aggtaggatt ttcatccagc ggcacccgga
    37201 agggtggttg aatatctccc cagggtctgc tcatccataa ggtaattgct ttcttatctt
    37261 catcccaaat gcaatagcct gcagtccatg ttggaaaacc caatgactga acctgctgaa
    37321 atagtatatt tgccgcctcc ggaagttcat tactcttctg cattgccatt gttcttgccc
    37381 tcactctctc cagcgctaat tgaatttgag attctcttac ctgtgcttct gctttttgaa
    37441 gatcaaggaa acgtttatag gtcatttcaa acaccgtagt gaacctgata agtaataact
    37501 catcttggac agaaagcaag gcatccgtaa ttatatgcag atagccctga gagaaattac
    37561 tgcaataaaa aatggtagga tcggcaaagc gggttctgac aaattcatca atttcagtgt
    37621 ctctgtaatg ctgtgttcca aattcaatat gccttttcag aagttcacct cctaccgact
    37681 gatgaaatat tttttctttc cttttccacg catcatacct ttcattaaac acctgttcgc
    37741 cggtaagagg aagccggacg gcatttactc cagaaccatc cacaacggtc atccatgcat
    37801 tctggatatt gttggtttca tctatttcca catatccgca attaataaac cggctaacac
    37861 caagtccccg cagttcatta tacaacaatt cagatacttc cacaagttct tcacttcgat
    37921 gcatcgccat ggttcttgca cgaactcttt ccaatgaagc ctctatctgt gcttctctag
    37981 cctgggcttc ggctttttgc agatcaagga aacgcacata ggcttgttca aagaccctgg
    38041 caaaccgtcc caggacgtta aattcctcct cggaaatggt ttggcccgaa taactgtcca
    38101 cccacaatgc cgaatgcttt tccgccacca acgattgcgt atagccgggc gcctgcattt
    38161 ggatctgcct tgctacctct ggtatcgtgt caatgtcatt gtgtctgtgg gcataggcga
    38221 aaaacttatt cttctcttga aaggagtaat gcttgctgaa attcttcccg ctttccttag
    38281 ccgtccagac atccctgatg atttcactat cttgatagtc atcttctatg aatggaagat
    38341 tcgcaagcac aggtgctgat gcaagtcccg gtgcggcgac ccattgaaca atgtccctgg
    38401 tgcctccggt gtacaattga atgcccgcca catcaaaggt caatcccagt tcctttagct
    38461 tttcaaacac tactaccacc acctctttca attcatcgct tttgtgcatg gctaaggaac
    38521 gggaacggac tttttccaaa gcggcttcaa tctgtgcttc acgggcttgt tcttcagccc
    38581 tttgaagatc cagaaagcgg atgtaggtct gatcaaatac tttggcaaaa cgctgtaata
    38641 cctttgaatg accagagtcc aattcttgat cgctaaccgc tacaatgcaa ccatgctttg
    38701 tgtaggcaca gcaaatcgaa cagcgcaggg tatccatcat catctgcttc gattcttccg
    38761 ggagaaacct aaaatcggtt aaagtaaaaa ccttcttatc atactctctt ttttctttgc
    38821 cccccaattc aaaaacttta taagacagcc cttcgttaaa cgctgccatg aatttatcat
    38881 gaaacgggtc ttccaagtag ggcaccttat aaatttgcgg caacacggac tgctcatcgg
    38941 aagacataaa ttcctggtaa cctctttttg attcgtcgag caacaaaatg gagcacataa
    39001 atgcttcaaa ccccaagcct ttaatttgct taaacaataa tgaaaccact tcgcgtaatt
    39061 catcgctttg gtgcatggcc atggttctgg cacgcactcg ttccaatgct aattgtatct
    39121 gcgcttcttt ggcctgtgct tccgcttttt gcaggtccag gaagcgggtg taggttcttt
    39181 caaattcgat ggcaaagcga tggagaatac tttgttcctg ctggctcaac ggccttcgaa
    39241 tatcaatccc gaagcatccg tatttcataa aagcctccgt gtagtaaatg ccttcaggga
    39301 agaacaccgg tttcagtcct tcggccccct ccagcagttc ctcccaggaa gcaaagaagg
    39361 tttcaatatt ttcgggagca ataaaatgct catgcaccgg gaccccgctg cgccaatcct
    39421 caaaacattt ggccgtataa ggttccgtaa gatcgagcgg ataggtaatg gccctggtgt
    39481 gaaaagcagc agttccatcc ttttcttgca tccaggtcgc ccagaataaa tgatctcctt
    39541 tttcttcatt gggcagccat acaaaagaga actcggaatc aattcctaaa agcagcaact
    39601 gttgatgaaa aaccttggaa atttcgagca gctcatcgga atgttgcatg agcatggtct
    39661 tggtacgggt gcgttccagc gccagttcga tctgggtttc cctgttacga ttctcggctt
    39721 ccttcaagtc ttcgaaacgc cggtaggcaa gatcaaatac gttggcaaac cgcaccaggg
    39781 tttttaggtc ctcttccgga gggtttgaaa ccacgcccgg caagctatac ccgatttctc
    39841 cgtgcatggt ccgggccatt atgaatgtga gcccttggat acggcggata tcgtcgtggg
    39901 atggcgcgtg gggatccacc agttcaaaat ccccaagggc caccatttta tccacataat
    39961 ttaaggccgc atccacatcc atggcataca ccagggctgg ttcttcgctt ttttcccaat
    40021 gggccaccag gggaatatcc ccatggatat gccggggcag gctcatgatc attccaatcc
    40081 gggtgccgtc gccggaagtc atggctttct cgtacctatc aggcagccaa cgcatatacc
    40141 agaaataatg cgcttcatgg cccaggcggg taaattcatt ccgcatggtt accacaatgt
    40201 ccagcaagtc gcccgattgc ctcatcgcca tggcctgaga acggactctt tcaagggctg
    40261 cttcaacctg ggcctcccgg gcctgggctt cggcattttt taaatcgagg aaacgggtat
    40321 aagtaagatc aaaaactttc gcaaagcgga gcaatatatc aaaatgctca ttggataagg
    40381 gctcaaggct tgcgacgttg ataccgccaa aattgttgaa tgatgcggac agtaaaactc
    40441 tctcaggtgc tttcatcccg ttctttaccg catcagaaag attggacagt tcggtctcat
    40501 tgaataaaag atcatcccaa tttattttat cctggccttt cagatcgtat acgaatttta
    40561 cattttgatt tttccactcc cgtacaaaag tgagataagc gggatgttca tggtgtttga
    40621 tataaaaact catcgggcgg gagggttctt cgctgttggc catccaccat ctcgctgcat
    40681 gggttgccgg ctcaaatacc cagatgatac aacgggtaag ggctaaatca agtttggtta
    40741 attcagtaaa tacggttccg ataagtgtac tcaattcttc agaggattgc atagccatcg
    40801 ccctggctct caccctttcc aatcccagtt caatttcagc ttctctggcc tgggcttccg
    40861 ctttttgcag gtcgaggaag cgggtatagg attgttcaaa aacttttccg aaccgtttta
    40921 atacttcgtt ttcttgttca ctgaatgctt taccatgatg gttggtgatg gaaatactgg
    40981 tgtgccgtga gattaccacc gaccgggcaa atcctcccgt acgcgacagc aaggcttcct
    41041 ttctttcctg tgaaaggctt tgccagggtt catattggaa gaggtggtcg aaaaatgtcc
    41101 tcttttcctg atcggtgaac gactctatca aaaacgtatt ccccttttta atggcatccc
    41161 tgatccgggt gaaaatgggt ttgtttagaa ccggaactac gattttttct acatagtccg
    41221 ccattcctcc agaggcccag atcgaaagtc cggtacccac ctcggcattg atacaaatga
    41281 aaacaccgcc gtcaatatcc atcccgatac ccagcaattg ttgtgcggtg acatttacca
    41341 cctcctgcag ttcggaggtg tgatgcatgg ccaggctgcg ggctcttact ctttccaatg
    41401 ccaattgaat ttccgcttcc tttgcctgcg cttcggcctg tttaaggtcg gcaaacctcc
    41461 ggtaagcgag tccaaaaaca ttggcaaatt ttctcagcaa acctctggta gcgtcatcgg
    41521 gagcctccat ggtaaccatc aacaatgacc ctccggtaaa atctgcaaac gaccagaatg
    41581 cccgcagatc cgcaatcttc ccttgtaccg aatccaccag tctggagtag cttcgggggg
    41641 attctttttc aaggaatgaa aaccattgtt tcagtttatc gccttcattg aggaggatgt
    41701 aatcctgccg gtgggaggag taagcctcca tggcttcttc gataatcctc aatccctttt
    41761 tgggaacccg ggccgtggaa tccacaagaa tttcgccttc cccttccggc ggccgaatgg
    41821 ctgcccagga atgtatataa tcggaagatt cctcatttaa atggatcaca caggtttcaa
    41881 gatccttttg ccctaataaa cccatttgtt ttctcagttc atgcaccact tcgttcaact
    41941 cctgcgagga atgcatggcc atcgcccttg ctctcactct ttccaatgcc gcctcgatct
    42001 gtgcttccct ggcctgggtt tcggctttct gcaaatcgag gaagcgggta taggtgccgt
    42061 caaaaacccc tgcgaatctt tccatcaaca acaaggtttc ccccgggatg ttcttatcgg
    42121 aagaaaagga caaccatcct ttggagaaaa aaacgatatt gacaatccaa tggtcctcag
    42181 gaaaattcac tttaatctcc aaaaatcgat tacggagttt gttgtattct tttaattccc
    42241 gcccatgaaa ttccaaggtc aacgaagagc gctttgcttt ccatgcggcc actgctttag
    42301 ccatcacatg cggatcgtcg atcgggacat ataaagtttt tggctcagga tgaccctgta
    42361 ccgtggtaga taattcaaca acccctgcgt cctccttaac gattcctata gagttttgca
    42421 cggccgtcaa tcccaattcc ttcatttgtt gaaaaagcaa aaaggacgtt ccaggcagtt
    42481 catcactttt ctgcatcgcc atggtacggg cacgtattct ttccaaggct aattgtatct
    42541 gcgcttcttt tgcctgcact tctgcatttt gcaggtcaag aaaacggata taagcctggt
    42601 tgaacaccct ggccgcccgg cggatcacgt caaggttctc ctcagggtaa ggttggccgg
    42661 tgaagctgtc agcaaacagc gccgaatgag attcggcgat caggcagaca taatagtcgt
    42721 ccaggtccct cagcacggtc cgggcgtttt ccgggatcct gatcaggtcg ttatgggaga
    42781 aaacaaaatc gatgaacctg tccttttgct gccgggagta aaaccggttg atgatcgcct
    42841 ggccggtgga gaaagcctgc cagccatctt tgaggtaagt gtcttgctgc atcaccccgt
    42901 catcgtgggg caggttgatc atttgcggct cctggagggc attgcccacc cacaatacgg
    42961 aatcgcccgt tcgctgatcg aacagctgaa tgccgacggt gcccagcagc acgttgagct
    43021 cgctcagctt ctcaaagaac acggcgatca ctgcgctcag ctcgctggaa tggtgcatgg
    43081 cgagggacct gcttcggatc ttttccatgg ccgcttcgat ctgcgattcc ctggcctgtg
    43141 cctcggcttt ctgcagatca agaaagcggg tataagtttg ctcaaatact tttccaaaac
    43201 gaaggaagat ctgtgtttca ttaaagggtt tcgtggtaat gaaaagcaga taaccgaatt
    43261 taaaggggac cgcatggttt ttttgccagg ctggaagggg atgaccactg tctataaatt
    43321 tttggatgcc ttgcttaaag gccggaatct gcataaacgc ttcataatat ttcttttgtc
    43381 tttctccttc gtatacctcc tcatacaggg ccgcctgatt tttccaggcc tcatacatgc
    43441 gttcctcgcc cggttcaatg ttatagggat ggttaaatac cccaatgctg gtccattttt
    43501 gaaccatgac gctgttttta tcgcatatgg caaatgccac tccctgcaat tcggcgccaa
    43561 gctgtttcaa ctggtcaaag aggagctctg ccgtctggat cagttccgag ctggtatgca
    43621 tggccatcgt tctgcttctc actctttcca gggccgcctc gatctgtgct tcccttgcct
    43681 gcgcttcggc tttttgcagg tcaaggaaac gggtataagt ttgttgaaat acgtttccaa
    43741 aacgtatcaa taggttgttc tcttcatccg tataaggaat ggcggaaaag ttttcaatgt
    43801 acaatccgac attgtcgagc agcaccgtgg aaacggcaag ccccttgcat tttaaataga
    43861 atttctgtgc ttcctccggt atcgtaaata gtttgaaaag ggatcgatag aatttgtttt
    43921 ttgttttaaa gtccagcagg tcggtgtgca ggatggttcc atttgctttg gcttccaaaa
    43981 agctgttcca ggtcggcgta tcgaaatagg gaatgactgc atagaatggc tcaagtttcg
    44041 ggtcggccag ccagatgtgc atgtcctcgt gggctttgta atctatataa aaaccggcat
    44101 gctctgcatt gatgttgagt tgaccaaatt gctgaagcac caggcgtatt acgtctttca
    44161 gctcatcact cctgtgcatg gccatgcttc ggcttcttac tctttccagc gccgcttcga
    44221 tctgtgcctc ccgggcttgt gcttcggccc gctgaaggtc caggaaacgg atatacgatt
    44281 gttcaaaagc ctttgcaaaa cgcctgaata ttccgacttc ctcggcggaa aacggaatgc
    44341 cttcgtaatt ggatatggca aaccagatgt ctttaaagta aaagagagtc ctggcaaatc
    44401 ctttccggtt taacacctgt tttttcctgg attccggtga atgtttagcc tcggaatttt
    44461 tgaaaaaatg ccggaagaaa acatttttaa ccttctgatc atagacgtca gaaatatgat
    44521 caagttgatt tttccgcatt tccaggaccc ggttaaagat cggatggtcc aggtaaggca
    44581 caaacagttg agccggatag gcttgtgccg gggtagaaag ccataaatcc cagttgccgg
    44641 cttcatccac tttcgcaaaa ttggcgacat caaattttaa ccggagcgag cacaattgtt
    44701 cagtcaccac cttgatgacc tggtgcaaat cgctgctctg atgcatggcc atggaactgc
    44761 ttcgcaccct ttccagggcc gcttcgatct gcgcctctct tgcctgtgct tcggattttt
    44821 gaagatcgag gaagcgggta taggtttgat caaaaaccct ggcaaaacgt ataaaaatat
    44881 cgtgagcggc gggaaccggt tcataggtga tgaaaaatag gaatcctgcg gagaagtaag
    44941 catagtgcat gatctggaaa tcgggaaggg gatagcctgc tttaatgacg gaatccagga
    45001 tctcccccac cacagggagc gttcgcatat accggtagtg ttttaccaat ttttcgcctc
    45061 ccatttccac cctgtgtaag ggctttcggt gttcgtgtcc cttcctcatc tgaataaaca
    45121 attcgtcctc cgtggtgggg gctgtaaaag gcggctgcag cgccccttcc gtactcatcc
    45181 acaaggtaac cgcggattta tcctcgttcc agatgcaata ccctgcgctc catgccggca
    45241 gttctagggt tttaacctgg tgaaacaaca gattggccgc ctccggcaat tcactgcttt
    45301 cttgcatggc catggtcctg gctctcactc tttccagggc caactgtatt ctggattccc
    45361 ttgcctgccc ttccgccttc tgcaaatcca aaaaccgttt ataagcaaga tcgaaggcag
    45421 cgccgatctt caataaaatg atttctgtct ccacctgcgg ctccctggtg aaatcggcga
    45481 tcaatatgcc gttggtcaat ttcaccccgc ggtgccagcc gtgttgtatc tta
//
LOCUS       DAOQXT010000028        44396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_953_length_44396_cov_5.805395, whole genome shotgun sequence.
ACCESSION   DAOQXT010000028 DAOQXT010000000
VERSION     DAOQXT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 44396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44396
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_953_length_44396_cov_5.805395"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..2678)
                     /locus_tag="PKM27_06810"
     CDS             complement(324..2678)
                     /locus_tag="PKM27_06810"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNR07006.1"
                     /translation="MIRFILTGGLGRMAWRGLMVSLLLVTFFDLQAQARKRKQKTAVV
                     PVMAPAPKPIVLTQEGMSRYRILLPEHASAYEEQAAEVLQSYLLEVSEAALPVIRSDQ
                     GINEFEIVLGQNERLNQLGKAVDLNALKEDGFLIRTDSMRLIIAGGTGKGTLYGVYSF
                     LEKYLGCRMYTPKVKIIPKQKNISLDHIYDQQVPKIEFRTTHYKVTWNKEYTDWHKLD
                     HDENGGRPDWGMWVHTFNELIPPEIHYKEHPEYYAEVRGKRIPTQPCLSNPEVLNITI
                     QNLRKKIAQNPEATYWSVSQNDNRDYCTCGQCKAIDEQEGSPSGSIIRFVNQVAAQFP
                     DKMISTLAYEYGRHAPKNLRPLENVNIMLCSIEALRHKAIDQDSASADFAKDLADWGK
                     ISKDIIIWDYVIQFPNLLSPFPNLHVLQPNLQLFVKHNVNAMFEQGNREVGGEFAELR
                     SYLLTKLMWDPDQNVDTLMNDFLNGYYGAAGGFIRHYIDVMREALLQSGRPLRIFGGP
                     NEAADSYLSPALMERYNNLFDAAEQAVAGQPEVLERVRIARIPIQFATMEIAKKNYKG
                     EGGLFIKKDNGWEVRPDIRSLIDPFADLCIRQGVTRVKEWSTSPEEYRASMYRLFYHG
                     MKEHLAYGKKIRLISPDTTKISTQSASMLTDGIRGGHDYAYNWITFQGQDLEAIVDLE
                     EVKTVSRMESAYYQLAMWLRIFPKQVEYYTSVDGTQFELAGTVQNTLPIDQYGAQQRD
                     FILNFSPRPARYVKIKAVTIGVTPEWHPGAGRAPYMSIDEIVVD"
     gene            complement(2860..6024)
                     /locus_tag="PKM27_06815"
     CDS             complement(2860..6024)
                     /locus_tag="PKM27_06815"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNR07007.1"
                     /translation="MKKYLLAVVLVFGWSPFLFCQKGKTTRMAVKPTTPVPEEPVYPF
                     DESHFKALKWRNIGPFRGGRVTAICGIPDQIYTFYMGSTGGGIWKTTDGGNRWTNVSD
                     GFVKTGSVGSIQVAASDPNVVVAGMGESPIRGVMTSHGDGVYKSTDAGRTWTHLGLQK
                     VRQISQIRIHPNDPDLIYVGAQGSPYEPTEDRGVYRSRDGGKTWQKILFVNKNSGVSD
                     LSMDLKNPRVLYAAFWDMQRQPWYIRSGGPGSGIWKSTDGGDTWKKLTTGLPKSIMGK
                     IGVSVSMANPQRVYAMIESDEGGLYRSEDGGNTWALINADRILRARAWYYTHVYADPQ
                     NPDRVIVLNAPYLESTDGGKTFKNVQVPHGDNHDLWINPHNNQFIANANDGGANISYN
                     GGLNWSEQSNQPTAQFYRVNADHRFPYWVYGGQQDNTAMAIQSRTAGPGISGKDWVVI
                     SGCESAWPAFDPDNPRYIYAGCYQGIIEEYDWETREVKDVMAYSFLGLGTKPADQKYR
                     FNWNAPILVSKFNPKTIYHGGNKVLKSEDRGISWTEISPDLTRNEVSKQGQGGGPITS
                     EGAGGENYNTLACLAESPLESNVLWAGSDCGLIHVTRDGGKSWANVTPADLKEGLVHS
                     IEASPHDPGTAYVAFTRYKFNDFTPYIYITRDYGKTWTLRTRGIGEEAHMRVVREDPA
                     RKDLLYAGTETGMYISFNGGAHWQPFQLNLPVVPITDLKVHQGDLIVSTAGRSFWILD
                     DLKPLHEYQPDLLSQKLVVYQPEPAYRFGGGMGNEYPGLGLNPPAGVVIYYHLNIKDT
                     ADQVTVQILDEKENIIRSYASSEKTAPKKAPRDSAMNRLVWDFRTEAQELPEGLLVLG
                     GNSGYRLAPGKYRVKVSYGNETANRDIEVRADPRHQTTPDQYNEQQSLLRELKSSLDD
                     VYDQVKRIRYIKDQIGGFTKRADLQDQDIKTLAGNLSRKLDSLENKMVQPRTKTFQDV
                     INFENQLDAKLKHIMDVVDESLPPVTQGQKTRARELINEWISLKQAVSNLTGEELKQL
                     NELIRKSPIPFISTEREAAKTGKS"
     gene            6244..8076
                     /locus_tag="PKM27_06820"
     CDS             6244..8076
                     /locus_tag="PKM27_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNR07008.1"
                     /translation="MVTEVKSTLLKGGEFLVRDASPDQTFIPEQFGEEALMVARSAQD
                     FLDQDIRPNQERIEKQEEGLSESLMRKMGELGFLGIHMPEAYGGTDLDTNTNTLVTDI
                     LGPMGSFSTTFAAHTGIGMLPILYFGTETQKQAYLPGLITGETIASYCLTEPGSGSDA
                     LSAKTRADLTEDGKSFILNGQKMWISNAGWADLFIVFAQVYGNKWKDGKGGFSCFLVE
                     AGKPGLTLGEEERKMGIKGSSTRQVYFENVKIDTDALLGEVGKGHKIAFNALNIGRFK
                     LGAFCIGGCKALITRSAQYANERVQFGKTIGSFGAIQYKLAEQLIRTYVSESTLYRTS
                     NLLQEKTTQLMAEGLSPEAAKLKAAEEYAIECSVLKVNASEALDYTVDEAVQVHGGIG
                     FSEENPVARAYRDSRINRIYEGTNEINRLLIVEMLLKRALKGEINLTDAAWSVQKELA
                     SMPSMDKATGAYADETSALRDFKKLVLMVAGAAVKMQMDGKLMLQDEQEIITNCADML
                     IDVLNAESLLLRIQRQESLDIRPDQKPLQEAILKVHFHDVNGRLAKNALDALASFCEG
                     DLLKTLAMGVKRFTKYPLVNVKEKRRAIAQAVLKSNKYPLSLFE"
     gene            8103..8234
                     /locus_tag="PKM27_06825"
     CDS             8103..8234
                     /locus_tag="PKM27_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07009.1"
                     /translation="MNTNKIEKSSVPEIAGDKWLEHWVEICEEDFASIEVDDFILID"
     gene            complement(8244..8903)
                     /locus_tag="PKM27_06830"
     CDS             complement(8244..8903)
                     /locus_tag="PKM27_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR07010.1"
                     /translation="MGKREIRQVMILDAAEKVFEAVGFANAKMETVAGEAGMSKGSVY
                     FYFQSKENLYMAITYRGMQKLNDLMYRCLAGHKENTGAAGVIGLLETYIDFAEDFPLY
                     IEALLDYMAVNRSAASGPDGAKLTEAMRESIYYQKIQDIQNIPISLAVGEIERGWLDG
                     SVKNKNKPELLFITAWATIVGYLKLTTASGKHRSTLHKIGRTEWKKYVLQLMKDQLEH
                     P"
     gene            9181..9528
                     /locus_tag="PKM27_06835"
     CDS             9181..9528
                     /locus_tag="PKM27_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNR07011.1"
                     /translation="MAIIITNECINCGACEPECPNNAIYEGGVEWAIGDGNTVNGPYT
                     LEDGTIVDAQQRNKPVEADYYFIVPDKCTECVGFHEEPQCAAVCPVDCCIPDTDRVES
                     REALLAKKERLHL"
     gene            complement(9606..10769)
                     /locus_tag="PKM27_06840"
     CDS             complement(9606..10769)
                     /locus_tag="PKM27_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR07012.1"
                     /translation="MKMKALSLFALISLVLVFTNCNRDQDLTLVPDDPSQAAQAWTPS
                     EGMSPFLSAETRAQMPAEDLEFVDRILKNPSEDMLRSRGAEVHIPAGSVNALQAAIDA
                     ANPGDVIVLDAGDHSESGSIKINKPVTLMGETGTKLIFSNLLPGATFTSAIQVVAGAE
                     QTVIRNIHFTSTDPISGVCIFADALSQVKILRNIFENFVFTIVGYQAQRFIISQNKIT
                     VGTGGAHGIMLSDGDQNIAVLNEISNGMFGIFAGGTNGISFSNTTNNNLYGQVLCKVP
                     PGQIIIDGTSLDTQGPTVNWSVKYNEAHNNLAAGYLVVDGSHNNFLIFNNGFGNGTYD
                     IELTSDTERFGFLAPASHNNRVVAYRWLTVKDCGDHNIVNGGKRVSIILDPCN"
     gene            complement(10952..12514)
                     /locus_tag="PKM27_06845"
     CDS             complement(10952..12514)
                     /locus_tag="PKM27_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07013.1"
                     /translation="MQNKSIRQREDAGWAQMRRQLDLDMPVHKKDRKIIWLFLFAGLG
                     LTGLIFFANRVFNSPEAPVLPQSRPLEETAKVSNPAENPSRDLSLDESQAAADTGGVN
                     SEKPETDATILSNDIEPVNQAVANHSSRTGQDAKTLLKARKSPSPSSTAIHREKGSTA
                     TGAETIKSSVPQKSTDLPAAVPEENLALNQKKTNPLPEVPTESEPKEEAAETASPKDE
                     QPKDELPAVAANPGIQASGISNSGNVNLIASAEAEMENPVEIKTENPRKRHGYLSMGA
                     DYAGDHFTSPYLTGGLRLPVSGRFFIAGQAGLSYQIKKGPSGSSNSTVTLSSQSYGKH
                     FLDAGNASSGSNTLDADDLFIDKNRSLTSFSGDSLLLTAGDQVTYYSSNQWMALAQLG
                     LGYRILPGLSIESGLHYRYYTGSFRDFVSIKSTNPFGNGFNTSSSATFKLNPAVSRQQ
                     FSLYLNTGLRLTNHLGLVMQVAGKPFSLSSSVSRVESAGLNTSASNQKTALVLPVKQN
                     SAYYRLGLTLSF"
     gene            complement(12504..13067)
                     /locus_tag="PKM27_06850"
     CDS             complement(12504..13067)
                     /locus_tag="PKM27_06850"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR07014.1"
                     /translation="MKLFKQAIPDDASLISGSIANDRKSQEKLYRKYFNRLFNMGLRY
                     TSDEDLVQEITHDAFLKAFKNLEKYQFAGSFEGWLRKIMFHCVVDHFRAKKSPRFIEL
                     DDIRQLFDESAPLPWETEELIGYINALPDNHKTVFNLFAIEGYSHQEIAEKMGISEGT
                     SKWYLNQARTILKGKIVQKKMNETYAK"
     gene            complement(13078..13827)
                     /locus_tag="PKM27_06855"
     CDS             complement(13078..13827)
                     /locus_tag="PKM27_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07015.1"
                     /translation="MKKGIGLVLLIFIIASAQAQDSVKYIRYKVVQRNGSSLVGQIME
                     KKADGPYVVVLDNGALVEIREKDVLRLKPVLPKDRVRRPYVEPSYKWGLSTEIMVVNN
                     GIQHIGEPGLATGASISAQRYLIPQLSTGLGLGLYNYDLGARRMFVPLFGEARYRLIR
                     HTVSPLLVIKGGYGFAAKNDINGLRKHEGGIFVNPFLGYEFGTGRKVSWTIGIGLLFQ
                     RAYYSYINGQDFFDEAIRFRRTEFKLGIAIH"
     gene            complement(13833..15110)
                     /locus_tag="PKM27_06860"
     CDS             complement(13833..15110)
                     /locus_tag="PKM27_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07016.1"
                     /translation="MKKIFLFWTLGLALALPSCLRDECVSHRVFVRMDPVYSTVQALR
                     DGGFSIAQARPLRHPGKIYSYGHYLLINEIDEGIHIIDNSDPAHPANLSFIQITGNRD
                     MAVKENILYADNVVDLLAIDITDPLAPVLRKRLENVFQKSYGWGGAAASQPLLVGYTK
                     SNETQVIDCAQPRFQRETFLDNSGFWVRADVLQSSGGNSAAKTNGVGGSMARFGIVDR
                     YLYAIDQSELHVVYIDQPADPKEGKSVSISWNIETLFPYKDKLFIGAQNGMFILENKN
                     PEAPYLLSSFSHARACDPVYVDGETAFVTLRDGNECTNYTNELDIVDISNITSPRLVK
                     KYQMQHPHGLSVIDQILFLCEGQYGLKAFDAGNIQTIDKNLKAHLTAFHAYDVIALSR
                     SHLIVVGEQGLIQLDASDLKNIKTRSLIPVVKD"
     gene            15283..16965
                     /gene="treS"
                     /locus_tag="PKM27_06865"
     CDS             15283..16965
                     /gene="treS"
                     /locus_tag="PKM27_06865"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HNR07017.1"
                     /translation="MERTSGWFKDAIIYQVHVKTYRDTNGDGIGDFSGLISKLDYIRD
                     LGVNTIWVMPFYPSPLRDDGYDIADYMRINPTFGKVEDLQTLIREAHARDLRVITELV
                     INHTSDQHPWFARAKKAPAGSSHRDFYVWTDNPEQYQGVRIIFRDFEPSNWSFDEETG
                     LYYWHRFFNHQPDLNFDNPEVQAEIFAIMDYWASMGVDGFRLDAIPYLYEREGTSCEN
                     LPETHAFLKKLRSYLDEKHPGLLFLAEANMWPEDSAAYFGEGDECHMNYHFPLMPRLY
                     LALKKADRGPIEYILNRTPQIPATCQWAIFLRCHDELTLEMVTDEDRQFMWENYSPEP
                     RARINLGIRKRLSTLMDNDLRRIKLMNALLFSMPGTPLVYYGDEIGMGDNIWLNDRDG
                     VRTPMQWNAGLNAGFSEADPSALYLPVIADEEFHFTKINVEAAEEDPYSLLHWMRRII
                     SIRKQYAAFGRGDFNFAEADHPSVLSYWRSYGVENILVVVNLSQVLQSTAIDLNKPGF
                     QAVLEDLFSGRRIHAENGQIKTELHPFEFLWLTEPRPELAVLVEDKLVLGLD"
     gene            complement(16970..18241)
                     /locus_tag="PKM27_06870"
     CDS             complement(16970..18241)
                     /locus_tag="PKM27_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNR07018.1"
                     /translation="MNSIETISEVYIPVTVDSPALIRQINALIPQVIYEDSSLVEDNM
                     TIRAEKMDSVDIRIFPNRLVYDVPLKLNIVRDVGITKVKADGALKLFFSTDYHIQPDW
                     SIQALTTILNHEWIEKPRVRLGVLQIPIENIASRIVRRSSDLICKQIDAQLQEGFKLR
                     DYIDQAWRKIQAPILITEKPHLSWLLLRPEKILMVPLNTHDSQIQTLLVFRSKTDLVF
                     GTKPVLPYAGILPTFEQIGQGPKDSLIELSLNFPLRRAEILLTDYFRGQEFRDGNRVM
                     MIDSIHLSGQGNKLQVETYVSGTYPAKLELEGVPVYNAEKRRFELAGLDYSINSRNLL
                     VKAASWFLKKNFQKKLAELLTYEVGGYMDSGKINLAEALGQVSSFGFRMEPVINDVWA
                     LHPVIQGDQATVTFLASGRFQIRIDRLVEEN"
     gene            18421..21609
                     /locus_tag="PKM27_06875"
     CDS             18421..21609
                     /locus_tag="PKM27_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNR07019.1"
                     /translation="MKKTLLQFYVLIYLFSVPFNLSAQKKKTTLPAKPAAAAPAPQSP
                     PMIYDTSLYAGMRWRSIGPYRGGRSCTVTGVPGKPNLYYFGSTGGGVWKTENGGNTWK
                     NVSDGYFGGSIGAVAVSASDPNVVYAGGGEKTVRGNTSFGYGVWKSEDAGNTWKSLGL
                     DKTRFIGRIRIHPTNPDIVFVAAIGNIFAPHLDRGIFKSTDGGKNWKKVLFVNDTAGA
                     VDLCFDPNNPRILFATTWKIRRTPYSLESGGAGSALWKSTDSGETWTNISANKGLPSG
                     VWGIAGVSVSPVNSDRVYALIENENGGVFRSDDGGKTWTTQSQDRNLRQRAWYYTRIY
                     ADTKDLDVVYVLNVSYHKSKDGGKTFSSFNANHGDHHDLWIAPEDPARMAIADDGGAQ
                     VTVDGGASWSTYHNQPTAQFYRVTTDNHFPYRIYIAQQDNTTLRIYHRTEGFAITEND
                     WEPSAGGESGHLAVDPLNNDIVYGGSYDGLLERTDHSKKIERSVDVHPDNPMGHGAEG
                     FKYRFQWNFPIFFSPHDPKKLYAASNHLHVTRDEGQTWQLISPDLTRNDLSKMGPSGG
                     PITKDNTSVEYYCTIFAAAESRRVRDLIWTGSDDGLVHVSRDGGNNWTKVTPPDMPEW
                     TMINCIEPDPFLDGGCYFAATGYKQGDFAPYIYKTEDFGKTWKKIVSGIPGEHFTRVV
                     RADPGRKGLLYAGTETGMYISFNDGESWQPFQMNLPIVPITDLAIKNNNLIAATQGRA
                     LWIIDDLSPLHQLSDQAAKSAIHLYKPMDAYRMGGYGGFRMPDLSLNGQNHPGGVLVN
                     YYLSEAPGKKDTITLAFHEENGRLIKTFSNFPKENEEKFEPAKGYNTFSWNLRYPNAK
                     RFDGLVLWAGSLNGPMAAPGKYKVEIKRKGAAQSHDFNILKNPVSSVTEDDIRRQVQF
                     VNENIQKLTETHQTIIDIRETRAQIKAYTEKIKDNPDMKDVIEKSKSIDSVMTRVEEA
                     LYQTKNRSGQDPLNFPIRLNNKLAYLNTITNRGDFPPTQSSLQVRGELVEKINAEIVR
                     WQEVQNKDIPALNALIKSKAIDPISIKKQAKP"
     gene            21647..22843
                     /locus_tag="PKM27_06880"
     CDS             21647..22843
                     /locus_tag="PKM27_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNR07020.1"
                     /translation="MGLSPDFSSMVTLCRAAMLVCLFLGSSLFLPGQDSLRISLLSCS
                     PGAELYSIFGHSAIRVTDEASGTDRVYNYGTFNFREKNFYLKFLSGKLNYYLSVQSFP
                     SFMSDYLEENRTVYEQVLNISHFYKLQLHTALRLNALPENRAYKYDFFWDNCSTRIRD
                     KIESSYADSLIYLPTPGVTFRDYLHQYLVHNPWSAFGIDLILGLPADKGTGPREAMFL
                     PLEMMKVFETARVDGQAVCSPAEIILPAQPLSESKSWFQPTWTFLLLLAVTAMAFLWF
                     PSTGASKFIANLLFISAGLAGLLIGFLWFIADHTTTDYNYHFLWANPLCLLYPWRRRL
                     LSEKTNFTISLLYVLILLLLIPGTLFLPQSLPVPCIFIWLALLLVLLPELKGRGRLRP
                     DLISHQ"
     gene            22871..25183
                     /locus_tag="PKM27_06885"
     CDS             22871..25183
                     /locus_tag="PKM27_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HNR07021.1"
                     /translation="MFFRSIVFVVVLVVIYSCRDEGTSRKAGQVDYAFDVKPILSDRC
                     YKCHGPDEAKREANLRLDTEEGAMALVGEKLDHVVIIRGKADSSELISRIYSNDPDFQ
                     MPPPDSKLTLTDQEKETLKKWIDQGAQWKPHWAFQKPVKSKLPEVGRKKWPRNEIDYF
                     TLAKMEEMGLHPSRQAEDHILARRMAFDITGLPPLPHLLDQLMASDAEDRFEVYIDSL
                     FRQPSYGENQASAWLDLARYGDTHGYQDDLPRVMWPWRDWVIHAFNRNMPYDQFVTWQ
                     LAGDLLPEANKEHLLASAFNRNHKITQEGGVIDEEYRVEYVADRTNTFGKAFLGLTME
                     CARCHDHKYDPISTEAYYQTFAFFNQVPETGFVQNQTTPKPWMPISKKDVEGILHFVN
                     TSKVIRNESDTIEQMVMKDDLQKPRKTYILKRGSYEEHGQEVHASALAVVLPYDTAAF
                     GSDRTGLAKWLFSKDHTLTSRVLVNRIWQHLFGRGIVATSDDFGSQGSLPTHPQLLDW
                     LAVDMIEQGWDIQYLMKKIMSSATYRQQSNADGRSAAQDPENKYLSRGPRYRMNYEMI
                     RDNVLAASGLLNREIGGPSVKPYQPPGLWEELTVGGDAFRGEATYKMDKGDDQYRRSL
                     YTYWRRTIPPPTMMTFDNPQREFCEVKRVRTSTPLQALVMLNDPQVLEASRVLAYRIM
                     ETYEDEDDRLKQMFKRVMSRLPASSETREILEYYKSQKALYEKDRASAVKLLSTGQSP
                     HPVNVDPAESAAWMMTCSLLFNLDESLTKY"
     gene            25189..26655
                     /locus_tag="PKM27_06890"
     CDS             25189..26655
                     /locus_tag="PKM27_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNR07022.1"
                     /translation="MMHGPDDEVIQFLMQQNRRTFLSKLSVGIGSLALGSLMCRSRAG
                     LEELAGGASVSSFQSIAPKAKRVIYLFQSGAPSQLELFDYKPLLNKMRGQDLPESIRQ
                     GQRLTTMTSGQKRFPLVGSSFAFGQHGESGAHFSELLPHMSGIADEITLIKSMYTEAI
                     NHDPAVTFLQCGHQQPGRPAMGSWLSYGLGSENENLPAFCVFLSRGKGNIQPLASRMW
                     GSAFLPSIHQGVQLRSGKDPVLYLDNPDGFDSGSRREMLDKLAALNQKQMEEMGDPEI
                     STRIAQYEMAYRMQTSVPEVLNLADEPTHILNLYGPDVLRPGSYAANCLMARRLSESG
                     VRFVQLYHMGWDAHSDLPETERNLTRDVDQPSAALIKDLKQRGLLDETLVIWGGEFGR
                     TNYCQGDLTRENYGRDHHPRCFTIWMAGGGIKKGYTHGETDEFGYNIIRDPVHVHDFQ
                     ATLLHLLGIDHEKMTFKYQGRRFRLTDVAGKVVDQVLA"
     gene            26782..28176
                     /locus_tag="PKM27_06895"
     CDS             26782..28176
                     /locus_tag="PKM27_06895"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNR07023.1"
                     /translation="MYFKPFRFLIVLIVLTISVSSCRNDQLDDITNSGKIAEVREFDS
                     EVPLAWYNIFLEVDRFSQGYAAPAAARLLAYVGLAAYEAAVPGMPEYQSLGVKFPGLT
                     LPKTEAESEYHWPAVLNSTYHQTFKKFYPHISQAYIDKMEALDKRIVSGFSLKIPGDV
                     LFRSAQRGRDIADAIYAWSLTDETGHWGFKNPQPADYNPPKGPGKWKPTPPEFNRALF
                     PFWSAVRPFTLGNEEIIGRPVQYWVGEFSEDPNSDFYKQAEEVYRMNFPVKDSLQWIG
                     EYWSDDIFELTFETAARWISISNQCVKAQECNLETSVLLYAKMGMALCDAAIVVWKTK
                     YAYNVESPVSYINRIIDPSWKPSLDNAITGMSGITPALPAYPSEHAGFAGAATSILSG
                     TFGSEFAMSDKSHELRTEFNGKPRTYKSFEEMAQENALSRLYLGVNYRMDTEEGLDLG
                     NRVAARVETLPWKK"
     gene            complement(28276..28716)
                     /locus_tag="PKM27_06900"
     CDS             complement(28276..28716)
                     /locus_tag="PKM27_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNR07024.1"
                     /translation="MLSTAPVKKEKMLMRFGKPTKRVLFTVDFLLKASPTIVYHFITT
                     PSALVRWFCDAVDITGEYYTFSWKGNTEVAELIDDIEDERLRFHWLDSEDEDEYFEFR
                     ITTADITDETILEIVDFAWADEVEEQKQLWRTQVERLRKETGDA"
     gene            28751..29239
                     /locus_tag="PKM27_06905"
     CDS             28751..29239
                     /locus_tag="PKM27_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07025.1"
                     /translation="MLGVAVFPAFGLFVLAAFPGAPAVAACAGLEDLSFTAGLAAFAE
                     GPVVRLFLAGLRAAGAGFVLAAFADALSVFTGFTAGFGGSGLRDLLALGAVAGFLLMA
                     GAVVAFPLAGLPFVPGFFILYSDCLYPAKKNKFLNCPFKKENFHFELLMHDIKQYFRR
                     MP"
     gene            29327..30196
                     /locus_tag="PKM27_06910"
     CDS             29327..30196
                     /locus_tag="PKM27_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNR07026.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVDLLTPEDEVTLAKLIK
                     QGDQAALERLTKANLRFVVSVAKQYQNQGLSLSDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKVGSLNKINRAFSELEQQFEREPSPEELAS
                     ILEIPSEEVETTLGVAARHVSMDAPFIDGEDNTLLDVLENNGTPSTDSALEYRESLRN
                     EIERSLGTLTDRQADVIKLYFGIGVEHPMSLEDIGEKFGLTRERVRQIKDKAINKLRT
                     ASRSKLLKHYLGS"
     gene            complement(30461..31639)
                     /locus_tag="PKM27_06915"
     CDS             complement(30461..31639)
                     /locus_tag="PKM27_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNR07027.1"
                     /translation="MNVKFQILSAGLMGGVIALGGFLLLTSKTGVGGDPLSLQPVSSA
                     WTRAANPAAPFDFTVAADKAKRSVVYIETKSNPLASQRRQDNLRRQTPRGLDEFFDLE
                     KMFRGFQFDLGPREGKGSGVILTADGYIVTNNHVIANADEINVKTFDDRKFVARKIGV
                     DPSTDLAILKVDANGLTPAEIGNSDALKVGEWVLAVGNPFNLDFTVTAGIVSAKGRNK
                     LNLIQDSKPIEEFIQTDAAVNPGNSGGALVDVSGKLVGINTAIYSPDGSFAGYSFAIP
                     VNLMQKVVTEIRQNGSVERVGSLGIEIHEIDEELAKEQNLPSKKGLLIKSVQEGSIAD
                     YGGLIPDDIILQVDGRDISTFDELVDIRDESRVGQTLMFKIMRDGEVKTLPVKLRSKI
                     "
     gene            31800..32513
                     /gene="trmB"
                     /locus_tag="PKM27_06920"
     CDS             31800..32513
                     /gene="trmB"
                     /locus_tag="PKM27_06920"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNR07028.1"
                     /translation="MSRKKKLEQFAELDRFENVLQCTDPQSSQAIRYDGEVVETRGHW
                     RDGIFKNNNPLVTELACGKGEYTVALAQRDPGKNFIGIDIKGNRMHRGAKNSLALGLG
                     NARFLRIRIEWLTHHFAPGEVDEIWITFPDPFLKKSKANRRLTSPGFQKMYRNILSPT
                     GTVHLKTDSPELYEFTLETLMSTPGVSIIAQSEDIDRDGLNEGILSIQTHYEKLHRAS
                     GISIRYISWIYHAGVELLN"
     gene            complement(32558..32833)
                     /locus_tag="PKM27_06925"
     CDS             complement(32558..32833)
                     /locus_tag="PKM27_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07029.1"
                     /translation="MFYSFIGLWLVVLALNKFMARQVAGNPKLSNALKAMVLIPMSLM
                     GYLLHPLIIRLVKDQVLISLVMMVALSGFAFVNLWIIGKIRRKGGKG"
     gene            complement(32863..33201)
                     /locus_tag="PKM27_06930"
     CDS             complement(32863..33201)
                     /locus_tag="PKM27_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNR07030.1"
                     /translation="MKKPFYFLLLLTAVFSLSAFTASAADQTSTFMVKGSCGMCKKKI
                     ETSLRVKGVKRVNWDKETKMLTVTFNPEHISLDEIHHRVAAIGYDTEKVKAPDEAYAK
                     LDDCCRYDRD"
     gene            33275..33751
                     /locus_tag="PKM27_06935"
     CDS             33275..33751
                     /locus_tag="PKM27_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07031.1"
                     /translation="MIQEETIDACIELVADSQQDWLRLMSEKQPVLASYLTSEDHEAF
                     SEGETQYLYYLAVMIWMCFDRTYPEMAEVDEETLALREEQNWNRVDSLRPASLKNIVD
                     RIMPDYPEQELLFYLEDALQMDEEDPEHPVTRAGQIPLFVTLLSISDALSEAATAG"
     gene            33886..34917
                     /locus_tag="PKM27_06940"
     CDS             33886..34917
                     /locus_tag="PKM27_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNR07032.1"
                     /translation="MSSKTYLFALTGLLFSLTARAQDIHFTMFDMSPLTLNPALIGDF
                     HGTFRITGNYRIQWPTIAKYKTPAFGIDAPLFRGLGKNDWISFGLAAYQDKAGDEFGL
                     KKTSGWIGGSYHLALKKDGSSLISIGVQSGGTSRSFDGIPRFQDPSDPAFPGSSGGGN
                     DNKRTFADINAGLLFRNALNKEKTSWLNMGLSLYHLSQPNSALGGGGGGTGQTPGQDK
                     QNVLINAHAQMIIPRGDLLQFVPGVVLHSAGGNFEVAGQLKGEMLINREKDFSVIGGL
                     GYRVSDAAFAIFGARFKDLKVALAYDFNISGATSSTSGIGGYELGVSYIAKIYKKPVV
                     KPVIFCPRF"
     gene            35103..36008
                     /locus_tag="PKM27_06945"
     CDS             35103..36008
                     /locus_tag="PKM27_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR07033.1"
                     /translation="MSILTLQQVSKTFQNYKAVDQISLTVPRGCIYGLLGPNGAGKTT
                     TIRMITGITHPDSGDIFLGGNRVNKTIPSSIGYMPEERGLYKKMSVWDHLLYLARLKG
                     LSVQKAKEELKFWIERLDMKSWYTKKVEELSKGMSQKAQFIASVIHKPDLLILDEPFS
                     GLDPINTNLIKDEISRLRDMGTTILFSTHRMEQVEEICERMVLINKGKNVLEGDVEEI
                     RKSHRQHLFELVSENDLPGRFPDEFHEIQRLGKKVRFNPEPHIQSADLLRYFLNEGIE
                     VNAFREQFPSINDIFIETVEKSNQA"
     gene            36005..37321
                     /locus_tag="PKM27_06950"
     CDS             36005..37321
                     /locus_tag="PKM27_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07034.1"
                     /translation="MNKLWLIIKREYTTRVFKKSFILLTLLVPLGIIAFSVIIGFIMS
                     RNTVQNIRVAISDPQQMVNDSLIRKNSEIQYIISDQPFESLKSGIQSGQYQAVLEVMP
                     PEDLKQKDFKIRINTDERLDINTTESIRNTLARQYRNYKIKNLQLNMDEINTLDVDIS
                     LDRHAVFNQDKKQSNVSNVVGAILGGIMGILMYIFVLGQGMMVMRSVMEEKMTRIVEV
                     ILSSVKPIELMMGKIIGVGAVGLTQLLIWIIFMPLAGIITTLMLGKNVFKPSMSGVNV
                     DQAIQQADSLGLENIMMELGQMNWWFIIPVFLLFFVFGYFLFASLCAAIGSAVGDDQS
                     EAQQLLLPITLLIVMGLYIAMAAIRAPYSNLAVISSFIPFFAPMVMPTRLAFDPPVWQ
                     TLLSLAILAISCYFFALLAAKIYRTGILMYGKKASFKEIGKWLFYK"
     gene            complement(37429..39471)
                     /locus_tag="PKM27_06955"
     CDS             complement(37429..39471)
                     /locus_tag="PKM27_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HNR07035.1"
                     /translation="MKRIICGFLLWFNYSTHVSSQAIDPWHLKVSSPDPSHYYGITSA
                     NGMIGIVSSPEPFKVKEVVLAGAYDLYGRGRVSNFIRSFNLLNMYLEIDGQRLDAGNI
                     RNMNQSMDLRHALFSASFDYMDKARITYSYAALRHLPYCVMMEVSVTALRAVKLTAAS
                     VMEAPDALREVENYYQEIDRPHVTIGLLTSKAKSPTGKMDLCASNTFLFEEGHGKEPR
                     TIHEMWDNNMHLMKFSKELAAGGSYRFALAGASITSAHHDDALNEAERLAIYARLEGK
                     ERLLKFHKEAWDRLWQSRILVEGDDQAQQDLNSMLYHLYAFTREGTSLSPSPMGLSGL
                     GYNGHVFWDTELWMFPALLVMQPALAESMLEYRFERLGAAKRNAFGKGFRGAMYPWES
                     ASTGVEETPVWALSGPFEHHITACVGIAAWNYYCVSQDKQWLREKGYPILKETADFWA
                     SRVERNGPGQYDIKNVVAADEWAENVDNNAFTNAAAKANLQFATQAARLLGLDINPDW
                     MHVASNIPILKFPDGVTREHASYQGEGIKQADVNLLAYPLKEITDPAAILMDLEYYKV
                     RVPDAGTPAMTQAVFTTLYSRLGRGEEAHKWFVESYQPNLLPPFRVIAETKGGTNPYF
                     ATGAGGILQSVLMGFAGIEITELGLNQIKTTLPPHWKSVRVLGAGPKRLEFINRKP"
     gene            39861..41309
                     /locus_tag="PKM27_06960"
     CDS             39861..41309
                     /locus_tag="PKM27_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNR07036.1"
                     /translation="MEEASLISGYRVESKVWDEMFDHQGIRPLYQKMVNAINRLSAEE
                     MNHKDDLAKKLFMSQGVTFTVYSSGEGIEKIFPFDIIPRLIGAAEWKHIETGIKQRLK
                     ALNIFLHDIYHDQFIVRDGLIPVQLIYSCPGFIRAMVGVKVPHQIYTHISGIDLIRDQ
                     DGQFFVLEDNLRTPSGVSYMIENRSITYRIFPDLLPRIGVRPVKDYPDLLLNNLLSLS
                     NRQTSAPTVVLLTPGIFNSAYFEHTTLARLMGIELVEGKDLVADGNKIYMKTINGLKQ
                     VDVIYRRVDDEFIDPLVFRRDSLLGVPGLYSAYRAGQVAVVNAIGNGVADDKAIYSYV
                     PEMIRYYLNEEPVLKNVPTYPMENPELRAMVFNDLSRMVIKKTNESGGYGMLIGSSAT
                     EKQMAEFRKAIELDPRSFIAQPIINLSSVPCYIQGRLQARRVDLRPYALYGPHGIDIV
                     PGGLTRVALKEGSLVVNSSQGGGSKDTWVCNE"
     gene            41346..42269
                     /locus_tag="PKM27_06965"
     CDS             41346..42269
                     /locus_tag="PKM27_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNR07037.1"
                     /translation="MLSRVAESIFWMARYMERTNGTLRLVRTSYISSQDEIHDFTWAP
                     ILQHYSDRSDFPPGDSKQVIEHLLLDKENASSVINNITRARENARAVQDHITKEVWQC
                     LNDFYHLIRNERIHAQILRGDAISAMDALIENGWLYHGVLDTTLARGNGFAFLSLGKF
                     IERAILSANNLSIKLAELDYHLDRPLEIPALRYLLYSLSGYELYLKTHRGNLNADLVI
                     QQILYHNHFPHSLLYCLVRIGRYLERLQPESLPEVYKNLDFLTGKLMNHLKYTPVDIR
                     KGKELGRFLDHIRSGLYEITEAMNKNYFGHF"
     gene            42285..43247
                     /locus_tag="PKM27_06970"
     CDS             42285..43247
                     /locus_tag="PKM27_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNR07038.1"
                     /translation="MTRYQLKHITRYLYPSPVRESINQIMLYPIEDALQDVKRHEIAI
                     SHQPAIEIFSDYFGNRTGIFSIPQPLSELQIQSTADVVVHESLLPADDQAASKQWSEL
                     RAFGSLASYIDFLWQEKSDRLDDLVAITKDLADPAKTPLTVAKDMSGFVYRHFEYKKG
                     ITDTETGVHEIWSMRAGVCQDFAHVLLAMLRLCGIPARYVSGYICPKNHEFRGEGATH
                     AWVEAFIPFEGWLGLDPTNNCIVSDRHIRLAVGRNFSDVTPVKGTYKGSSEHRLEVGV
                     EIINGHSSDRPVISDAPPVITYQAHQASQNENSYRRAMEMQQQQ"
     gene            43607..44254
                     /locus_tag="PKM27_06975"
     CDS             43607..44254
                     /locus_tag="PKM27_06975"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNR07039.1"
                     /translation="MIPPADQYTLPPLDYAFGALEPHIDAKTMEIHHDRHHAAYVTNL
                     NKELSTHAAAAALPLEKLFAQSKKYSAGIRNNGGGHYNHSLFWKLLKPAASSTPIGAL
                     ATAINAKFKDLANFKDLMNKAGTGRFGSGWAWLVLDNKNNLVIGSTPNQDNPLMDVAE
                     VKGYPLLGIDVWEHAYYLKYQNKRADYLSAIWNVINWDEVAKRYEEGMKRNKKKG"
BASE COUNT        10808 a        11770 c        11061 g        10757 t
ORIGIN      
        1 cgatctcttc tcctgccttc atggaaccaa tagtcaatag tcaatagtca atagtcaata
       61 gtcgatagtc aatggtcaat aatcaatagt caatggtcaa taatcaatag tcaatggtca
      121 ataatcaata gtcaatagtc gatagtcaat aatcagtggt cagtagtcag taatcaatag
      181 tcacctgtat ttttgctccc ttaaccttta acccttcacc gttcgccttt ctcgcctctc
      241 gcttctcgta tctcgctact ggcatctcga atctcatcaa gtagttatgc tcccttaacc
      301 cttaaccctt aaccttttac cttctaatcg acgacgattt catcgatgga catataaggc
      361 gcccttcccg caccgggatg ccattccgga gtcacaccga tggtcaccgc tttgattttg
      421 acatagcgcg cgggccgggg tgaaaaattc aggataaaat ccctttgctg agccccgtac
      481 tgatcaatgg gcagggtgtt ttggaccgtg ccggccagtt caaactgggt cccgtctacg
      541 gaagtgtaat actccacctg tttggggaaa atgcggagcc acatggccaa ttgataataa
      601 gcggattcca tcctgcttac cgttttgact tcttccaggt ccacgatggc ttcgaggtcc
      661 tggccctgga aggtaatcca gttgtaggcg taatcgtgcc cgccccggat gccatccgtg
      721 agcattgagg cggactgggt ggaaattttg gtcgtgtccg ggctgatcag cctgattttt
      781 ttaccatagg ccaggtgttc cttcatgccg tgataaaaaa gacggtacat ggatgcacgg
      841 tattcttcgg gcgaggtgct ccattccttc acccgggtca ccccctggcg gatgcataaa
      901 tcggcaaaag gatcgatcag gctgcggata tccggcctca cttcccaacc gttatccttt
      961 tttatgaaga gccctccttc tcctttgtaa ttctttttgg caatttccat cgtggcaaac
     1021 tgtatgggga tccgcgcgat ccggacccgc tccaggacct ccggttggcc ggccacagcc
     1081 tgctccgcag cgtcaaagag attattgtac cgttccatca atgcgggact gaggtaactg
     1141 tcggccgctt cgttggggcc tccaaaaatg cgcaggggcc gtccggattg caacagagct
     1201 tcccgcatga catcgatata atgccggata aagcctcccg ccgcaccgta atagccgttg
     1261 agaaaatcgt tcatcagtgt gtccacattc tgatcgggat cccacatcag tttggtcagg
     1321 agataagatc tgagttcggc aaattcgccg cccacttccc gattgccctg ttcaaacatg
     1381 gcattgacgt tgtgttttac gaacaactgt aaattcggct ggaggacatg aaggttggga
     1441 aaggggctga gtaaattggg gaactgaatg acatagtccc agatgatgat gtccttggaa
     1501 atcttccccc agtcggccag gtcctttgca aagtcggcgc tggcgctgtc ctggtcgatg
     1561 gccttgtgcc gcagggcttc aatgctgcac aacatgatgt tgacattctc caggggcctc
     1621 aggtttttgg gagcatgccg tccgtattca taagccaggg tggagatcat cttgtccgga
     1681 aactgggcag ccacctggtt gacaaatcgg atgatcgatc cggacggcga gccctcctgc
     1741 tcatcgatgg ccttgcactg gccgcaggta caataatccc ggttgtcgtt ctggcttacg
     1801 gaccaatagg tcgcttcggg attctgggcg atttttttcc ggaggttctg aatggtgatg
     1861 ttcaacactt ccgggttaga caggcagggt tgggtgggta ttcttttgcc ccttacctct
     1921 gcataatatt ccggatgttc cttataatga atttcggggg ggatcagctc attgaaggta
     1981 tgtacccaca tgccccaatc cgggcgaccc ccgttttcat cgtgatccag tttgtgccag
     2041 tcggtgtatt ctttgttcca ggtcacttta taatgggtag tccggaattc gatctttggg
     2101 acctgctggt cgtaaatatg atccaggctg atattctttt gtttcggaat gattttcact
     2161 ttaggcgtgt acatcctgca accgaggtat ttctccagga aggaatagac cccgtacagg
     2221 gtaccttttc cggtacctcc ggcgatgatc agcctcatac tgtccgtccg gatcaaaaac
     2281 ccgtcctctt tcagggcatt caggtccacc gctttcccca actggttcaa tcgctcgttc
     2341 tgccccagta cgatttcaaa ttcgtttata ccctggtccg accggatgac cggcaatgcg
     2401 gcctcagaga cctccagcaa ataagattgc agcacctccg ccgcctgttc ttcataagcg
     2461 ctggcatgtt cgggtaggag gatccggtaa cggctcatcc cttcctgggt gaggacaatg
     2521 ggtttgggtg ccggcgccat caccggtacg actgccgttt tttgtttcct ctttctcgcc
     2581 tgtgcttgca ggtcaaagaa agtcactaat aatagggaca ccatgaggcc cctccaggcc
     2641 atgcgcccca ggcctccggt taaaatgaat ctgatcatat gaatatggac tttggaaaaa
     2701 caatatacag ctttcgacta tcagcaatca gctttcagct ttcagcaatc agcaatcagc
     2761 tatgagtact catgctcctt tcgccgtttc cctttctcca ttcctccctt tctcccttcc
     2821 tccctcccgc ggctgcggga ctccctgcct ccggcagcct cagctcttcc ccgtcttcgc
     2881 cgcctccctt tccgtcgaaa taaacggaat aggactcttg cggataagct cattgagttg
     2941 tttcaactcc tccccggtga gattggagac cgcttgtttc aggctaatcc attcgttgat
     3001 cagttccctg gctcgggtct tttgcccctg ggtcaccgga gggaggctct catcgaccac
     3061 atccatgatg tgtttgagct tggcgtcgag ctgattttca aaattgatca catcctggaa
     3121 ggttttagtg cgcggctgga ccattttatt ttccaggctg tcgagtttgc ggcttaaatt
     3181 tcctgccaag gtcttaatgt cctggtcttg gagatccgcc ctcttcgtaa acccgccgat
     3241 ctgatctttg atataccgga ttcgttttac ctggtcgtaa acatcatcca acgatgattt
     3301 taattcccgg agcagggatt gctgttcgtt atactgatca ggagtggtct gatgtctggg
     3361 atcggccctg acttcaatat cccggtttgc ggtttcattt ccataactga cttttaccct
     3421 gtatttcccg ggggcaagcc ggtatccgct gttgcccccc aagaccaaga ggccctccgg
     3481 caattcctgg gcttcggtcc tgaagtccca gaccaggcgg ttcatggctg aatccctggg
     3541 ggctttttta ggagcggttt tctcggaaga ggcataagat ctgatgatat tttctttttc
     3601 atccaggatc tgaacggtta cctggtcggc ggtatccttt atattcagat gataatagat
     3661 cactactccc gccgggggat tcaggcccag tccgggatat tcattaccca tgcccccgcc
     3721 aaaccggtat gcgggctcag gctggtaaac caccaacttt tgactcagca ggtccggctg
     3781 gtattcgtgg agcggtttga gatcatccag gatccagaaa gaacggccgg cggtggagac
     3841 gatcagatcc ccctggtgta ctttgaggtc ggtgatggga accaccggaa ggtttagttg
     3901 aaaaggttgc cagtgggcgc cgccgttgaa tgagatgtac atgcccgttt ccgtacccgc
     3961 gtaaagcagg tctttccggg cagggtcctc acgcaccacg cgcatatggg cttcctcccc
     4021 tattccccgg gtgcgaagcg tccaggtttt gccatagtcc cgggtgatgt agatatatgg
     4081 ggtgaaatca ttgaatttat acctggtaaa ggccacataa gccgttccgg gatcgtgggg
     4141 cgacgcttca atggaatgca ccagcccttc tttcaggtcg gccggagtaa cattagccca
     4201 tgatttcccg ccatccctgg tcacatgaat caggccgcag tcagaccctg cccagagcac
     4261 attggattca agcggggatt cggccaggca ggccagggta ttgtagtttt ctcctcccgc
     4321 tccttcactg gtgatgggcc ccccgccctg tccctgtttg gagacttcat ttcgggtcag
     4381 gtcgggactg atctccgtcc agctgatgcc ccggtcttct gatttcagga ctttgtttcc
     4441 tccgtggtag atcgttttgg gattgaattt ggatacgagg atgggcgcgt tccagttgaa
     4501 ccggtacttc tggtccgccg gtttggtgcc caggcccagg aatgaataag ccatcacatc
     4561 ctttacctcc cgggtttccc agtcgtattc ttcaataatg ccctggtaac aacccgcata
     4621 gatgtatctc ggattgtccg ggtcgaaggc cggccaggcg ctctcgcaac ctgaaatgac
     4681 cacccagtct ttacccgaaa tgcccgggcc cgcggtcctg ctctggatcg ccatggccgt
     4741 attgtcctgc tggcctccgt acacccaata aggaaagcgg tggtctgcat ttacccggta
     4801 aaactgggcg gtcggctgat tggattgttc actccagttg agcccgccgt tgtatgaaat
     4861 attggcgcct ccgtcgttgg cgtttgcaat gaactggttg ttgtggggat tgatccagag
     4921 atcatgattg tccccgtgtg gcacctggac gtttttgaag gtcttcccgc cgtcggtgga
     4981 ttcaaggtaa ggggcattga gcacgatcac ccggtccgga ttttgaggat ctgcatagac
     5041 atgggtgtag taccaggcgc gggcgcgaag gatccggtcc gcattgatca gggcccaggt
     5101 atttcctccg tcttcagaac ggtagaggcc gccttcatcg gattcgatca tggcgtagac
     5161 gcgctgggga ttggccatgg aaacagaaac cccgatcttg cccatgatgg attttggcag
     5221 tcctgtggtg agtttcttcc aggtgtctcc cccatccgtg gatttccaga ttccgctgcc
     5281 gggcccgccg ctgcgaatat accagggctg tctttgcata tcccagaagg cggcatagag
     5341 aaccctggga ttcttcaggt ccatgctcag gtcactgacc ccggagtttt tattcacaaa
     5401 gaggatcttc tgccaggtct tgcctccatc cctgctccgg tagactccac ggtcctccgt
     5461 aggctcataa ggacttccct gggctcccac atagatcagg tccgggtcat tgggatggat
     5521 cctgatctgc gagatctgcc tcactttctg cagcccgaga tgcgtccagg tacggcctgc
     5581 atccgtggat ttgtaaacgc cgtccccgtg tgaggtcatc actccccgta ttggggattc
     5641 gcccatgccg gctacgacga cgttgggatc ggatgcggcc acctggatgg aaccaacgga
     5701 accggttttg acgaatccgt ccgatacatt cgtccaccgg ttgcccccat ccgttgtttt
     5761 ccagatcccc cctccggtcg agcccatata aaaggtgtag atctgatccg gaatgccgca
     5821 aatcgcggtt acccggcctc cccgaaaggg gcctatattc cgccatttca aggctttaaa
     5881 atgggattcg tcaaagggat aaactggctc ctcagggacg ggtgtggtag gtttcacagc
     5941 catccgggtg gtttttccct tttggcaaaa taaaaacgga ctccatccaa acaccaggac
     6001 gactgccagg aggtattttt tcatttggta ttatttacgg taaaataaga aatcggccgg
     6061 atatattttg ggtccatacc ggctaccccg ggacgttaaa gtcatttatt ctgacttaag
     6121 gtcaataatt taattattgg aaatccttaa ttttcactac aaatgttaac aagtcgcaaa
     6181 aggcattgat tgatataaaa atatttttta actttgagtc aaattcaatt aaaccctaat
     6241 atcatggtta cagaagtaaa atccaccctg ctcaaaggag gggaattcct cgtccgtgat
     6301 gcttctccgg accagacttt tattcctgaa cagtttggtg aagaggcttt gatggttgcg
     6361 cgaagcgccc aggatttcct ggaccaggac atccggccca atcaggaacg catcgaaaag
     6421 caggaggagg gtttgtctga atccctgatg cgaaaaatgg gcgaactggg atttctgggc
     6481 attcacatgc ctgaagccta tggtgggacc gacctggata ccaataccaa taccctggtg
     6541 accgatatcc tcgggcccat gggttccttt tcgaccactt tcgcggccca taccgggatc
     6601 gggatgttgc cgatccttta ttttggtacg gaaactcaaa aacaggccta tctgccgggg
     6661 ctgatcaccg gggagacgat agccagttat tgcctcaccg agcctggttc gggatccgat
     6721 gccctgtctg caaaaaccag ggcggacctc accgaagacg gaaaaagttt tatcctgaac
     6781 gggcagaaaa tgtggatttc caatgcgggc tgggccgatt tgtttattgt ctttgcccag
     6841 gtgtacggta ataaatggaa ggatggcaaa ggaggttttt cctgtttcct ggttgaggcc
     6901 ggcaagccgg gtcttaccct gggagaagaa gagcgaaaaa tgggaattaa aggttcctcc
     6961 acccgccagg tctatttcga aaatgtaaaa atagataccg atgccctact tggcgaagtc
     7021 ggtaaagggc ataaaatcgc tttcaatgcc ctcaatatcg gccgcttcaa actgggcgcc
     7081 ttctgcatcg gcggctgcaa agccctcatt acgcggagcg cccaatatgc caatgagcgc
     7141 gtccagtttg gcaaaaccat agggagtttt ggggcgatcc aatacaaact ggccgagcag
     7201 ttgatacgca cttatgtcag cgagagcacg ctgtaccgga cctccaacct tctccaggaa
     7261 aaaacgacgc aactcatggc cgaagggctc tctccggaag cggccaaatt aaaagctgcg
     7321 gaagaatacg ccatcgaatg ttcggtgctc aaagtgaatg catcggaagc cctcgattat
     7381 accgtggacg aagcggtcca ggtccatggc ggaattggtt tttccgaaga aaacccggtt
     7441 gcccgcgcct accgggacag caggatcaac cggatctacg agggcaccaa tgaaatcaat
     7501 cgcctgctga tcgtggaaat gttactgaaa cgggccctca aaggggaaat caacctcacg
     7561 gacgcggcct ggtccgtaca gaaagaactg gcatccatgc catccatgga taaggccacc
     7621 ggggcctatg ccgatgaaac ctcggccctc cgggatttca agaaactggt attgatggtg
     7681 gcgggagcgg cggtaaaaat gcagatggac ggcaaactga tgctgcagga cgagcaggag
     7741 atcatcacca attgcgcgga catgctcatc gacgtactca atgccgaatc cctgttgttg
     7801 cgaatccaac ggcaggagtc cttggacatc cggccagacc agaaacccct gcaggaagca
     7861 atactcaaag tccactttca cgatgtcaac ggccggctgg ctaaaaacgc gctggatgcg
     7921 ctggccagtt tttgcgaagg cgacctgctg aagaccctgg ccatgggagt caaaagattc
     7981 accaaatatc ccctggtcaa tgtcaaagaa aaaagaagag cgattgccca ggccgtcctc
     8041 aaatcaaaca aatatccctt atcccttttt gaataataat aaccccattt aactcatttc
     8101 ccatgaatac aaacaaaata gaaaaatcaa gcgtacctga aatcgccggt gataaatggc
     8161 tggagcactg ggtggaaata tgtgaggagg attttgcctc cattgaagtc gatgatttta
     8221 tcctgatcga ctgagtctcg tggtcaggga tgctccaatt ggtctttcat cagttgaagc
     8281 acatactttt tccactccgt ccggccgatc ttatgcaggg tggaacggtg ctttccggat
     8341 gcggtggtca gtttgagata acccacaatg gtggcccagg ccgtgatgaa caggagttcc
     8401 ggtttgtttt tgtttttgac gctcccgtcc agccatcccc tttcgatctc cccgacggcc
     8461 agggatatcg gtatgttttg aatatcctgg atcttttgat aataaatgct ttcgcgcatg
     8521 gcttccgtaa gtttggcgcc gtccgggcct gatgccgcgg atcggtttac ggccatataa
     8581 tccagcaggg cttcaatata caaggggaaa tcttcggcga agtcgatata ggtctccagc
     8641 aggccgatta cgcccgctgc gccggtgttt tctttatgcc ctgccaggca ccggtacata
     8701 aggtcattca gcttttgcat gccccggtag gtaatggcca tgtacaggtt ttccttgctc
     8761 tgaaaataaa aatacacact gcctttactc atgccggctt ccccggccac cgtttccatt
     8821 ttagcattgg caaagccgac ggcttcaaag actttctccg ccgcatccag gatcatgacc
     8881 tgcctgattt ccctttttcc cattgatgac acaaaaatga taaatccaat cgatttgaag
     8941 ccaatatttt gacccggagt ttaaaaccgg gtcaaaccgg gtttccggcc tgaaaaacat
     9001 attttaaatg atgcacagga ggcggagtcc aaggttccca agacggccct atccattgaa
     9061 tgacatacct gtaagaaaga cgatcttaca acgacctctt ccgccccccg gcagcctcat
     9121 gttttaccta tctttgccga accaaaaccc cggacggggt gttaagagtg agaatttatc
     9181 atggcaataa ttataactaa tgaatgcatc aattgcggcg cctgtgaacc ggagtgtccc
     9241 aataatgcga tctatgaagg aggggtcgaa tgggccatcg gcgacggcaa cacggtcaac
     9301 gggccttata ccctggaaga cgggacgata gtggacgccc aacaacgcaa taaaccggtg
     9361 gaggccgatt attatttcat cgtgccggac aaatgcaccg aatgcgttgg ttttcacgaa
     9421 gagcctcaat gtgcggcggt ttgtcctgtg gattgttgta tcccagatac cgaccgcgtg
     9481 gagagcaggg aagccctgct tgccaagaag gaaagattgc atttgtaaac cgggatcctt
     9541 taaacgaaaa agcaaccccc gggaagggag ttgctctcgt gttatgatag catgggattg
     9601 tccctttaat tacaaggatc caaaattata ctgacccgct tgccgccatt tacaatattg
     9661 tggtctccgc aatcttttac agtcagccag cggtaggcca ccacccgatt attatgtgaa
     9721 gctggtgcaa gaaaaccgaa tcgctcggtg tcggaggtaa gttcaatgtc ataagttcca
     9781 ttgccgaacc cattgttgaa tatcagaaaa ttattatgtg aaccatccac gacgaggtaa
     9841 ccggcagcca ggttgttatg tgcttcattg tactttacag accagttgac ggtggggccc
     9901 tgggtatcca aagaagtgcc gtcaatgata atctggcccg gaggcacttt gcaaagaacc
     9961 tgcccataga ggttattatt cgtggtgttg ctgaatgaaa ttccattggt accaccggca
    10021 aagattccaa acataccatt ggatatttca ttcaaaactg caatattctg atctccatcc
    10081 gacaacatga tcccgtgcgc acctcctgtt cccaccgtga tcttgttttg gctgataatg
    10141 aacctctgag cctggtagcc cacaatggta aaaacaaagt tttcaaaaat gttccgcagg
    10201 attttcacct ggcttaatgc atccgcgaaa atgcatactc ccgatatggg atcggtggat
    10261 gtgaaatgga tatttctgat caccgtttgt tcagcgccgg ccactacttg gatggctgag
    10321 gtaaatgtcg cacccggcag cagattgctg aaaatcagtt tggtgccggt ttcgcccatc
    10381 agcgtgaccg gtttattgat cttgatggaa ccggactcgc tatggtcgcc ggcatccagg
    10441 acgatgacat cccccggatt ggcagcatca atggccgcct gaagtgcatt caccgagccg
    10501 gctggtatat ggacttcagc tccccgggat ctcagcatat cctcactggg attcttcaga
    10561 atgcggtcca caaattcaag atcttcagca ggcatttggg cccgggtttc cgcagacaaa
    10621 aaggggctca tgccttctga aggcgtccag gcctgtgctg cctgtgaagg atcgtcaggg
    10681 accagtgtca gatcctgatc ccggttgcaa ttggtaaaga ccagaacaag gctgatcaaa
    10741 gcaaaaagag aaagggcttt cattttcata tgaactaaat tttattggtt ttttctcggg
    10801 acatcctttg gatcagttca tccttaggaa tatttaaaaa tggtgagaaa aacctggccc
    10861 tttatgaaaa tttgataacc ggtgcaatag ctggataacc ggcacttggc atgtataact
    10921 gccggaaagg ccgggctccc ttagcccggt ttcagaaaga cagggtcaaa cccagacggt
    10981 aataggccga attttgtttg acaggcaata cgagggccgt cttttgattt gaagcggaag
    11041 tatttaaacc cgctgattcg acccggctca ctgaggaact gaggctgaat ggcttgccgg
    11101 ccacctgcat gaccaaaccg aggtggttgg tcaaccggag gcccgtattc aaatagaggg
    11161 aaaactgctg cctggatacg gccggattaa gtttaaaggt agccgaggat gaagtattga
    11221 atccattgcc aaacgggttg gtcgatttga tgcttacaaa atcgcggaac gacccggtgt
    11281 aataacggta atgaagaccg gattcaatgg aaagtccggg cagaatccgg tatcccagtc
    11341 ccaactgagc cagggccatc cactgattgg aagaataata ggtgacctgg tcccccgcgg
    11401 tgagcaataa tgaatccccg ctgaaggaag taagagacct gtttttatca ataaaaaggt
    11461 catcggcgtc cagggtattg cttccggaag aggcattgcc cgcatccagg aaatgttttc
    11521 cataagactg gctggaaaga gtcaccgtgg aattggagga ccccgatgga ccctttttga
    11581 tctggtaact caggccggcc tgccctgcaa taaagaatct tccggacacc ggcagcctga
    11641 gacctccggt gaggtaaggg cttgtaaaat gatcgcccgc atagtccgct cccatcgaca
    11701 gataaccatg gcgtttgcgg ggattttccg ttttgatctc gaccgggttt tccatctcag
    11761 cctctgcaga ggcgatgagg ttgacattgc cggaattcga aatgccggaa gcctgtatgc
    11821 cgggattcgc tgcgacggca ggcagttcat cctttggctg ctcatcttta ggacttgcgg
    11881 tttccgccgc ttcttccttt ggttcggatt ctgttgggac ttcgggcaaa ggatttgttt
    11941 tcttttgatt caaggccagg ttttcttccg gaactgcggc aggcaagtct gtagattttt
    12001 gaggtactga gcttttgatc gtttccgcac cagtcgctgt actccctttt tcccggtgaa
    12061 tggcggttga ggaaggcgaa ggagacttgc gggctttcaa taaagttttt gcgtcttgtc
    12121 cggttcggga agaatggtta gcgacggcct ggttgaccgg ttcaatatcg tttgaaagaa
    12181 tagtcgcatc ggtttccggt ttttctgaat ttaccccccc ggtgtccgcg gcggcttggg
    12241 attcatccag ggagaggtcc cgggatggat tctccgcggg attggacact tttgcagttt
    12301 cttccagcgg ccggctttga ggcaaaaccg gggcttccgg tgaattaaaa acccggttgg
    12361 caaagaagat cagcccggtc agtcccaggc ctgcaaataa gaacaaccaa atgatttttc
    12421 tatccttttt atgcacaggc atatcgagat ccagctggcg cctcatttgt gcccaaccgg
    12481 catcttctcg ctgccttatt gatttatttt gcataggttt cattcatttt tttttgaaca
    12541 atttttcctt tcaggatggt cctggcctgg ttgagatacc atttgctcgt gccttcgctg
    12601 atccccattt tttccgctat ttcctggtgg gagtagcctt cgatggcaaa aaggttaaac
    12661 accgtcttgt gattatccgg caaggcgttg atataaccga tcagttcttc ggtttcccag
    12721 ggcagtgggg ccgattcatc aaaaagctgg cggatgtcgt ccagttcaat gaaccggggt
    12781 gattttttag cccggaaatg gtccaccacg caatggaaca tgatcttccg gagccaacct
    12841 tcaaaggagc cggcaaactg gtacttctcc aggtttttaa aggctttcaa gaaagcgtcg
    12901 tgggtaattt cctggaccag gtcctcgtcc gaagtatatc gcaaacccat attgaacaga
    12961 cggttaaaat atttccgata caacttctcc tggcttttcc ggtcattggc tatagaccct
    13021 gagatcaggc tggcgtcgtc gggtatggct tgtttgaata acttcatcgg cggtttctca
    13081 gtgaatggct attcccagtt taaattccgt tcgccggaag cggatggctt catcgaaaaa
    13141 gtcctggcca ttgatatagc tgtaataggc tcgctgaaac aacaatccga ttccgatcgt
    13201 ccaggacacc tttcggcccg tgccgaattc gtaacccagg aacggattga caaagatacc
    13261 accttcgtgt tttctgagtc cgttgatatc gtttttagcg gcgaatccgt accctccctt
    13321 gatcaccagc agcggactga ccgtgtgccg gatcagccgg tagcgggctt ccccgaaaag
    13381 tggaacaaac atcctcctgg ctccaaggtc gtaattgtaa aggccaagac ccaggccggt
    13441 actgagttgc ggaataagat accgttgggc agagatgctg gccccggtgg ccaatccggg
    13501 ctccccgata tgctgtatgc cgttgttcac gaccatgatt tcagtgctga ggccccattt
    13561 atacgatggt tcgacataag gcctccttac cctgtccttt ggcaaaacag gtttgagccg
    13621 gaggacgtcc ttttccctga tttccaccag ggccccgttg tcgaggacga ccacataggg
    13681 gccgtctgct tttttttcca tgatctgccc gaccagggac gatccgtttc gctggacgac
    13741 tttatagcgg atgtatttga cgctgtcctg ggcctgcgcc gaggcgatga taaaaatcaa
    13801 taataccagc ccgattccct ttttcatatc cttcagtctt taaccaccgg gatcaatgac
    13861 cgggtcttga tatttttcaa atcggaggca tccaattgga tcaggccctg ctcgccgacc
    13921 acgatgaggt gggacctgga cagggcaatc acatcgtaag catggaaggc ggtcaggtgc
    13981 gctttcaggt ttttatcaat ggtttgaata ttcccggcat caaaggcttt taaaccgtac
    14041 tgaccttcgc agagaaaaag gatttggtca atcacggaaa ggccgtgagg gtgctgcatc
    14101 tggtattttt taaccaaccg gggtgaggta atatttgaaa tatccacgat atccagttcg
    14161 ttggtataat tcgtgcattc gttgccgtcc cgcagggtga caaaagcggt ttcgccgtcc
    14221 acatagaccg ggtcgcaggc acgtgcgtgg gaaaaactgc tgaggagata gggggcttcc
    14281 ggatttttat tttccaggat aaacatgccg ttttgggcgc cgatgaaaag tttgtcctta
    14341 taagggaaaa gggtttctat gttccagctg atggagaccg atttgccttc tttgggatcc
    14401 gcaggctgat cgatatagac cacatggagt tcggactggt caatggcgta caggtaccgg
    14461 tccacaatcc cgaaacgggc catggaccct cctaccccat tcgttttggc ggcggaattg
    14521 ccgccggagg attgcaggac gtcggccctg acccaaaatc cggaattatc caaaaaggtc
    14581 tccctctgaa aacgaggctg ggcgcaatcg atcacctgcg tctcattgct tttggtataa
    14641 cccaccagca agggctgact ggctgcagct cctccccatc cgtaagattt ctgaaaaacg
    14701 ttttcgagcc gtttccggag gaccggagca agggggtccg taatatcaat ggccagcagg
    14761 tccaccacgt tgtcggcata aaggatattt tctttgacgg ccatatcccg gttgccggtg
    14821 atctggatga aggacaggtt ggccggatga gccggatccg aattgtcgat aatatggatc
    14881 ccctcgtcaa tctcgttgat cagcagatag tggccgtagg agtatatttt tcccggatgg
    14941 cggagcggcc tggcctgggc aatgctaaaa cccccgtccc ggagggcctg tacggtggaa
    15001 tagaccgggt ccatgcggac aaaaacccgg tgactcacac attcatccct caggcaggaa
    15061 ggaagggcga gcgccaggcc gagggtccaa aaaaggaata tttttttcat atgcatcgga
    15121 ttagataatc aggtagacgt ccggaaaagg tccggggttg gggatttgga tacgaggggt
    15181 gagatttaaa tggaaaatct ggaggcaggg aggacgccgt atctacagtt tgccttatta
    15241 ttgctgcacg actcaccatt tattatcaaa ttcgtaaagc gtatggaaag gacaagcggt
    15301 tggttcaagg atgccatcat ttatcaggtc cacgtcaaaa cttaccggga taccaacggg
    15361 gacggcattg gggatttctc aggacttatc agcaaactgg attatatccg ggaccttggg
    15421 gtgaacacca tctgggtgat gcccttttat ccaagccccc tgcgcgacga cggatatgat
    15481 atcgcggact atatgcgcat caatcccacc ttcggcaagg tggaagacct tcaaacctta
    15541 atccgtgaag cccatgcgcg ggacctgcgg gtcatcaccg aactggtgat caaccatact
    15601 tcggaccagc atccctggtt tgccagggcc aagaaagcgc cggcgggctc ctcccaccgc
    15661 gacttctacg tgtggaccga caatccggaa cagtaccagg gagtgcggat catattcagg
    15721 gactttgagc cttccaactg gtcttttgat gaagaaaccg gattgtatta ctggcaccgt
    15781 ttttttaatc atcagcccga cctgaatttt gacaatccgg aggtccaggc ggaaatcttc
    15841 gccatcatgg attactgggc ctcgatgggg gtggacggct tccgtctgga tgccatcccc
    15901 tacctgtacg aaagggaagg aacctcctgt gagaacctgc ccgaaaccca tgcctttctt
    15961 aaaaaactca ggtcctacct ggatgaaaaa catccgggat tgttattcct ggccgaagcc
    16021 aatatgtggc ccgaagactc cgcggcctat ttcggcgaag gcgatgaatg ccatatgaac
    16081 tatcattttc cgctcatgcc aagactttac ctggctttga aaaaagcgga ccggggccct
    16141 atcgaataca tactcaaccg gacgccgcag attcccgcca cctgccagtg ggccattttc
    16201 ctccgttgcc acgacgaact caccctggaa atggtcaccg acgaggaccg tcagtttatg
    16261 tgggaaaatt attctcctga accccgggcc aggatcaacc tgggcatccg gaaacggctg
    16321 tccaccctta tggacaacga tctgcgaagg atcaaattga tgaatgccct cctgttctcc
    16381 atgccgggta cacccctggt ctattacggg gatgaaatcg gtatgggtga caatatctgg
    16441 ctgaatgacc gcgatggggt tcgcacgccg atgcaatgga atgccggact caatgccggt
    16501 ttctcagagg cggatccgtc cgccctttat cttcccgtga tcgcagacga ggaattccat
    16561 ttcaccaaaa tcaacgtgga agctgccgaa gaagacccct attccctcct gcactggatg
    16621 cgaaggatta tttccatccg gaaacagtat gcagccttcg ggcgggggga ttttaatttc
    16681 gcggaagccg accacccttc ggtcctttcc tattggagaa gctatggcgt tgaaaacatc
    16741 ctggtggtgg tcaatctgag ccaggtgctc caatccacgg ccattgacct gaataaaccc
    16801 ggcttccagg cagtcctgga agacctcttc agcggacgcc ggatccatgc cgaaaacggg
    16861 caaatcaaaa cggaactgca tcccttcgaa tttttgtggc tgaccgagcc aaggcctgaa
    16921 ctggctgtgc tcgtggagga caaactggta ttggggctgg attgatcctt tagttttcct
    16981 ccaccaaccg atcgatgcgg atctggaagc ggccgctggc caggaaggtc accgtcgcct
    17041 gatcgccctg gatgaccgga tgaagggccc agacgtcgtt gatcaccggt tccattctga
    17101 atccaaaaga gctgacctgg ccaagggctt ccgccaggtt tattttcccg ctatccatat
    17161 atcctcccac ttcataggtc agtaattcgg ccagtttttt ctggaaattt tttttcagga
    17221 accaggatgc ggccttcacc aacaggtttc ttgaattaat ggaatagtcc aggccggcca
    17281 gttcaaacct tcttttttca gcgttataaa ccggtacgcc ttccagttca agttttgcag
    17341 gataggtgcc gctcacataa gtttccacct ggagtttatt gccctggccc gacaagtgga
    17401 tggaatcgat catcatcacc cggttgccat ccctgaattc ctggccacgg aaataatccg
    17461 tcagcagaat ttccgcccgc ctgaggggga aattaaggga caattcgatc agggaatcct
    17521 tggggccctg tccgatctgt tcaaaggtgg gcaggatccc ggcatagggc aagaccggtt
    17581 tggtcccaaa caccaaatcg gttttggagc gaaaaaccag cagcgtctgg atctggctgt
    17641 cgtgggtgtt caggggcacc attagaattt tttccggccg cagcaggagc caggaaagat
    17701 gtggtttctc cgtaatgagg atgggcgcct ggattttcct ccaggcctgg tcaatgtaat
    17761 cccgcaattt aaatccttcc tggagctggg catcgatttg tttgcagatc aggtcgctgc
    17821 tcctgcggac aatacggctg gcaatgtttt cgatggggat ctggagcacc ccaagcctga
    17881 cccggggttt ttcaatccat tcatggttca ggatggtagt cagggcctgg atggaccagt
    17941 ccggttggat gtggtaatcc gtgctgaaaa aaagcttaag cgctccgtcg gccttgacct
    18001 tggtgatccc aacatccctc acaatattga gtttgagggg aacatcgtaa accagcctgt
    18061 ttgggaagat ccggatgtcc acactgtcca ttttttcagc ccggatggtc atattgtctt
    18121 caaccaaaga actgtcttca tagatgacct gggggatcag cgcattgatc tgacggatca
    18181 gtgccgggga atccacggtg accgggatat atacttctga aatcgtctcg atggaattca
    18241 tgtcaggcaa atttgaagga accggggaga tggctgcagg ggcaggcagg gtcaccaccg
    18301 gaatggattt cctggcacag cccaaaattc ctcctgtcaa aatgaaaatt acgataatcc
    18361 tcctgcaatt cggaggcaat cgaaaaagct ttgtaatttt cattcttaat caggtgaaat
    18421 atgaaaaaga cgttgctgca attttacgta ttaatatatt tattttcagt cccttttaac
    18481 ctctccgccc aaaagaaaaa aaccacctta ccggccaaac cggccgccgc agcgcccgct
    18541 cctcaatccc cgcccatgat ctacgatacc tccctatacg cgggcatgcg gtggagaagc
    18601 attggcccat accggggcgg gagatcctgc acggtcaccg gggtaccggg caaacccaac
    18661 ctctattatt tcggcagcac cggcggcggt gtctggaaga ccgaaaacgg aggcaatacc
    18721 tggaagaacg tcagcgatgg ttatttcggt ggaagcatcg gggcggtcgc cgtctcggct
    18781 tcagatccga atgtagtgta tgccggtgga ggggaaaaaa ccgtaagggg aaatacctcc
    18841 ttcggatatg gagtttggaa atccgaggat gccgggaaca cctggaaatc cttaggactg
    18901 gataaaaccc gttttattgg gcggatacgc atccatccca ccaatccgga catcgtcttt
    18961 gtagccgcca tcggcaatat tttcgcgccg catctggacc gcggcatatt taaaagcacc
    19021 gatgggggta aaaactggaa aaaagtactc tttgtaaacg ataccgcagg agccgtggac
    19081 ctttgttttg acccaaacaa tcccaggatc ctctttgcaa ccacatggaa aataaggcgt
    19141 actccataca gcctggaatc cgggggtgcc gggtcagccc tttggaaatc cacggacagc
    19201 ggagaaacct ggaccaatat ttcggccaat aaaggacttc cgtccggagt ctggggaata
    19261 gccggggtca gcgtctcacc ggtaaattct gaccgggtct atgccctgat cgaaaatgaa
    19321 aacggcgggg tcttccgttc ggatgacggc ggaaagacct ggaccacaca aagccaggac
    19381 cgcaatctgc ggcaaagagc ctggtattat acaaggatct atgcggacac caaagatctg
    19441 gatgtggttt acgtgctgaa cgtgtcatat cacaaatcca aagacggcgg caagactttc
    19501 tcttccttta atgccaacca tggagaccac cacgacctgt ggatcgcccc cgaagacccg
    19561 gcccggatgg ccatagccga cgacggcgga gcccaggtga cggtagatgg cggagccagc
    19621 tggtccacct atcacaacca gccgacggcc caattttacc gggtgaccac cgacaaccat
    19681 tttccatatc gcatctatat agcccagcag gacaatacca cccttcggat ctatcaccgg
    19741 accgaaggat ttgccatcac ggaaaacgac tgggaaccat ccgccggagg agaaagcggt
    19801 cacctcgccg tcgatcccct caacaacgat attgtttacg gaggcagtta tgatgggctc
    19861 ctggaaagaa ccgaccattc caaaaaaata gaacgatcgg tggatgtcca cccggacaac
    19921 ccaatggggc acggagcgga aggtttcaaa taccggtttc agtggaattt tcccattttc
    19981 ttttcccccc acgatcccaa aaaactctac gccgcttcca atcacctgca tgtaacgagg
    20041 gacgaaggcc agacctggca actgatcagc ccggacctca cccgcaacga cctttccaaa
    20101 atggggcctt caggggggcc gatcaccaag gataatacca gtgtggaata ttattgcacc
    20161 atcttcgccg ctgcggaatc ccggagggtc agggacctga tctggaccgg ttcggacgac
    20221 ggactcgtcc atgtgagccg ggacggaggc aataactgga caaaagtgac cccaccggat
    20281 atgccggaat ggaccatgat caattgcatc gagccggatc ctttcctgga cggaggttgt
    20341 tatttcgccg ccaccggata caaacagggt gattttgcac cctatatcta taagaccgaa
    20401 gattttggca agacgtggaa aaaaatagta agcggcatcc ccggtgaaca ttttacaagg
    20461 gttgtccgcg cagaccccgg ccgcaaaggt ttgttgtatg ccggtaccga aaccggcatg
    20521 tatatttcct ttaatgatgg tgaaagctgg cagcctttcc aaatgaacct tcccattgta
    20581 ccgatcaccg acctggccat taaaaacaat aacctgattg ccgctaccca gggccgggct
    20641 ctatggatca tcgatgatct cagccccttg catcaactca gtgaccaggc ggccaaatcg
    20701 gccatccacc tgtacaagcc gatggatgca taccggatgg gtggttatgg aggtttccgc
    20761 atgccggatc tcagcctcaa cggccaaaac catcccggcg gcgtcctggt caattattat
    20821 ttaagcgaag cccccgggaa aaaagatacc ataacgctcg cgtttcatga agaaaacggc
    20881 agattgatca aaaccttttc caattttcca aaagaaaacg aagagaaatt tgaaccggcc
    20941 aaagggtaca ataccttttc ctggaacctc cgttatccca atgcaaaaag gtttgacggc
    21001 ctcgtactct gggcaggcag ccttaacggt ccgatggcgg cgcccgggaa atacaaggtg
    21061 gaaatcaagc gaaagggagc cgcgcaaagt catgatttta atattttgaa aaatccggtc
    21121 agttcggtta cggaagacga tatccgcagg caggtccagt ttgtcaatga aaatattcag
    21181 aagctcacgg aaacccacca gaccatcatc gacatccggg aaacccgcgc acagatcaaa
    21241 gcctatacgg aaaaaataaa ggacaatccg gatatgaaag acgtgataga aaaaagcaaa
    21301 tccattgatt cggtcatgac ccgggtggaa gaagccctct accagactaa aaaccggagc
    21361 ggccaggatc ctctgaattt ccccatccgg ctaaacaata agctggccta tctgaatacc
    21421 attaccaacc ggggcgactt cccccctacc cagtcctcat tgcaggtacg aggggagctg
    21481 gtggaaaaaa taaatgcgga aatcgtaagg tggcaagagg tgcagaacaa ggatattccc
    21541 gcgctaaatg ccctgattaa atccaaggcc atcgacccga tttccattaa gaaacaggcg
    21601 aaaccttgat ttttccgtcc ctcatttcta aaccggattt gatgatatgg gattaagtcc
    21661 ggattttagt tccatggtta ccctatgcag ggctgcaatg cttgtatgcc tttttttggg
    21721 aagcagcctg ttcctgccgg gccaggacag cctgcgcatc agtcttttgt cctgcagccc
    21781 cggcgccgaa ttgtacagca tattcggcca cagcgccatc cgggtcacgg acgaggcctc
    21841 cggcaccgac cgggtttaca attacgggac gtttaatttc agggagaaaa atttttatct
    21901 gaaattcctg agcggcaagc tgaattatta tttgtcggtg caatcctttc cttcgttcat
    21961 gtcggattac ctggaagaaa accgcaccgt ttacgaacag gtattgaata tttcccattt
    22021 ttacaaactg cagcttcata ccgccttgcg gctcaacgcc ctcccggaaa acagggccta
    22081 taaatatgat tttttctggg acaattgttc gacccggatc cgggacaaga ttgaaagttc
    22141 ttatgcggac agcctgattt acctccctac tccgggggtc accttccgcg actacctcca
    22201 ccaatacctg gtccacaatc cctggagcgc cttcggcatc gacctcatcc tcggattgcc
    22261 tgcggacaaa gggaccgggc ccagggaagc catgtttctt ccgcttgaaa tgatgaaggt
    22321 atttgaaacc gcccgggtgg acgggcaggc cgtttgttcc cctgcggaaa tcatccttcc
    22381 ggctcagccc ttgtcagaat ccaaaagctg gttccagcct acctggactt tccttttatt
    22441 gctggcagtc acagccatgg ccttcctttg gttcccctca accggagcct cgaagtttat
    22501 agccaacctg ttatttatat cagcaggcct ggcggggctg ctcatcggtt ttctttggtt
    22561 tatcgccgac cacaccacca ccgattataa ttatcatttc ctttgggcca atcccctatg
    22621 cctcttgtat ccctggaggc gccgcctgct ttcagaaaaa acaaacttca ccataagcct
    22681 cttgtacgtc ctgatccttc tcctcctcat cccgggaacc ctcttcctac cccaatccct
    22741 gcccgttccc tgtattttta tctggctcgc cttgctgctg gtcctgcttc ctgaattaaa
    22801 aggaaggggc cggctgcgtc cagatttgat atctcatcaa taatctttta attttagcga
    22861 aaatccttgc atgtttttcc ggtcgattgt gtttgttgtg gtcctggttg tgatatattc
    22921 ctgccgggac gagggaacca gccgtaaagc gggacaagtg gattatgcct ttgatgtaaa
    22981 acccattttg tccgaccgtt gttacaagtg ccatggaccg gatgaagcca aacgggaggc
    23041 taatctccgg ctggatacgg aagaaggggc tatggccctg gtcggggaga aactcgatca
    23101 tgtggtcatc atacggggaa aagctgattc cagcgaactg atcagtcgga tttacagcaa
    23161 tgaccccgac ttccaaatgc ctcctccgga ttcgaaactg acccttacgg accaggaaaa
    23221 agaaacattg aaaaaatgga ttgaccaggg tgcccaatgg aaacctcact gggctttcca
    23281 gaaaccggtc aaatccaaac tgcctgaagt gggccgcaaa aaatggccgc gaaacgaaat
    23341 cgattatttt accctggcca aaatggagga gatgggcctc catccttcca ggcaggcgga
    23401 agaccatatt cttgcacgtc gcatggcttt tgatatcacc ggtttaccgc ccttgccgca
    23461 cctgttggat caattgatgg cttcggatgc ggaggaccgc tttgaagttt atatagactc
    23521 gcttttcagg cagccttcct atggagaaaa ccaggccagc gcctggcttg accttgcccg
    23581 gtacggggat acccatggtt accaggacga cctgccgcgc gtcatgtggc cctggaggga
    23641 ctgggtgatc catgccttca accggaatat gccttacgat cagtttgtga cctggcaact
    23701 ggccggggac ttgctgccgg aagccaacaa agaacatttg ctcgcctccg cattcaaccg
    23761 gaaccataag atcacccagg aaggaggggt gatcgatgaa gaataccgcg tggaatatgt
    23821 cgccgaccgt accaacacct tcggaaaagc cttccttgga ctcaccatgg aatgcgcccg
    23881 ctgccatgat cacaaatacg atcccatttc cacagaagcc tactatcaga catttgcttt
    23941 tttcaaccag gtgccggaaa ccggctttgt gcagaatcaa acgaccccta aaccctggat
    24001 gcccatcagc aaaaaggatg tggaaggaat cctccacttt gtaaacacca gcaaggtgat
    24061 ccggaatgaa agcgacacca ttgaacagat ggtcatgaag gacgatcttc agaaaccccg
    24121 caaaacatat atcctgaaaa ggggtagtta tgaggagcat ggccaggaag tgcatgcctc
    24181 cgctttggcg gtggtcctgc cctacgatac cgccgccttc ggctccgatc gcaccgggct
    24241 tgccaaatgg ctgttctcca aagatcatac cctgacttcc agggtcctgg tcaaccggat
    24301 ctggcaacac ttgtttggcc ggggaatcgt ggccacctca gatgacttcg gcagccaggg
    24361 atcccttccc acccaccctc aattgcttga ctggctggcc gttgacatga ttgaacaggg
    24421 ctgggatatc caatacctga tgaaaaagat catgtcctcc gccacctacc ggcagcaaag
    24481 caatgccgat ggccggtccg cggcccagga tcctgaaaac aaatacctgt cgcggggccc
    24541 ccgataccgg atgaactatg agatgatccg cgataatgta ctggcggctt caggcttgct
    24601 caaccgggag attggcgggc cttcggtgaa accataccag cccccgggcc tctgggaaga
    24661 gctgaccgtg ggcggagatg ccttccgggg ggaagccacc tataagatgg acaaagggga
    24721 cgatcaatac cgcaggagcc tgtacaccta ttggcgcagg accattccac cgccaaccat
    24781 gatgaccttt gacaacccgc agcgcgaatt ctgcgaagtg aaaagggtga ggaccagcac
    24841 cccgctccag gcattggtca tgctcaacga cccgcaggta ttggaagcca gccgggtcct
    24901 ggcctaccgg atcatggaga cctatgagga cgaggacgac cggctcaaac aaatgttcaa
    24961 aagggtgatg tccagattgc ctgcaagttc ggaaaccagg gagatcctgg aatattataa
    25021 atctcaaaaa gccctttatg aaaaggaccg ggcttccgcc gtcaaattat tgtccaccgg
    25081 gcaatctccc caccccgtca atgtggatcc ggcggaatca gcagcctgga tgatgacctg
    25141 ttcattatta tttaaccttg atgaatcttt aaccaaatac tgatatccat gatgcatggc
    25201 cccgatgatg aagtaatcca gtttctgatg cagcaaaacc gaaggacctt cctttcgaaa
    25261 ctcagtgtcg ggatcgggag cctggctctc ggatccctga tgtgccgctc cagggcgggc
    25321 cttgaagaac ttgcaggggg ggcctcggtg agcagtttcc aatccattgc ccctaaagcc
    25381 aagcgggtga tttacctgtt tcagagcggc gcgccgtccc aactcgagct gttcgattac
    25441 aaacccttat tgaataaaat gaggggacag gatcttccgg aatcgatccg gcaggggcaa
    25501 cgcctcacca ccatgacttc agggcaaaaa cgatttccat tggtcggttc ttcgtttgca
    25561 ttcggtcagc atggagaaag tggcgcccat ttcagtgaat tgctgccgca catgtccggc
    25621 atagccgatg aaatcaccct gatcaagtcc atgtacaccg aggcgatcaa ccatgacccg
    25681 gcggtgactt ttttacaatg cggacatcaa caacccggca ggcctgccat gggctcctgg
    25741 ttgagttatg gcctgggcag cgaaaacgag aacctgccgg ccttctgtgt cttcctttcc
    25801 aggggaaaag gaaatattca acccctggcc tcccgcatgt ggggcagtgc ttttctcccc
    25861 tccatccacc agggggtgca gcttcgttcg ggcaaggacc cggtgctgta tctggataac
    25921 ccggatggat tcgattccgg gagccgccgc gaaatgctgg ataaactggc cgctttaaat
    25981 caaaaacaaa tggaggaaat gggggatccc gaaatttcga cccggattgc ccaatatgaa
    26041 atggcctacc gcatgcaaac ctccgtcccg gaagtgctta acctcgctga tgagccaacc
    26101 catattctca atctttacgg tcccgatgtg ctgaggccgg gaagttatgc cgccaattgc
    26161 ctgatggccc gaagactttc cgaaagcggg gtccgcttcg tccagcttta tcacatgggt
    26221 tgggatgccc attccgatct cccggaaacc gaaagaaacc tcacccggga tgtggaccag
    26281 ccttccgccg ccctgatcaa agatctcaaa caaaggggct tgctcgatga aaccctggtc
    26341 atctggggag gagaatttgg cagaaccaat tattgccagg gcgacctgac cagggaaaat
    26401 tacgggaggg atcaccaccc ccggtgtttc accatctgga tggcgggcgg aggcattaaa
    26461 aagggatata cccacgggga aaccgatgaa ttcggctaca acatcatccg ggacccggtc
    26521 catgtccacg atttccaggc caccttgctg cacctgcttg gtatcgatca tgaaaaaatg
    26581 acctttaaat accagggccg gaggttcagg cttacggatg tagccggtaa agtcgtggac
    26641 caggtgctgg cctgaaaaca tattgataaa aattgcggag gtcgcttttt tttaattccg
    26701 cgatttctta gctttgtgta tccaaaagac agctattgaa ctagctacca attatttagg
    26761 catcatacaa tttgtaatcc catgtatttt aaacccttcc ggttcctgat cgtattgatt
    26821 gtactcacca tttcggtatc ttcctgcagg aatgatcagt tggacgatat caccaattca
    26881 gggaaaattg cggaagtccg ggaattcgat tccgaagtgc ccctcgcctg gtacaatatt
    26941 ttcctggaag tggaccgctt ttcacaggga tacgccgctc ctgccgcggc ccggctgctc
    27001 gcttatgtcg gacttgccgc ctatgaagcc gccgtaccag gcatgccgga ataccaatcc
    27061 ctgggcgtta aattccccgg gctgaccctg cccaaaacag aagccgaaag cgaataccac
    27121 tggcctgccg tactcaacag cacctatcat cagaccttta aaaagtttta cccgcatatt
    27181 tcccaggcct atatcgataa aatggaggcc ctggataaaa ggatcgtgtc tgggtttagt
    27241 ctcaaaatcc cgggggacgt ccttttccgg tccgcccagc gcggaaggga tattgccgac
    27301 gcgatctacg cttggtcact caccgatgaa accggtcact ggggttttaa aaatccccaa
    27361 cccgcggatt acaatccgcc caagggtccg ggcaagtgga aacctacccc tcctgaattc
    27421 aatagggcgc ttttcccctt ctggagcgcc gtcaggccct ttaccctggg aaacgaggaa
    27481 atcattggaa ggcccgtgca gtattgggta ggtgaattca gtgaagaccc caattccgat
    27541 ttttacaaac aggcggaaga agtgtaccgg atgaattttc cggtaaaaga cagtttgcaa
    27601 tggatcgggg aatactggag cgatgacatt tttgaactga cctttgaaac ggccgcccgg
    27661 tggatttcca taagcaacca gtgcgtaaaa gcccaggaat gcaacctgga gaccagcgta
    27721 cttctttacg ctaaaatggg catggccctt tgcgatgccg ccatcgtggt atggaaaacc
    27781 aaatatgcct acaacgtgga aagcccggtc agttatatca accgcatcat cgacccttcc
    27841 tggaaaccca gcctggataa tgccatcacc ggcatgtcag gcattacccc cgctctgccg
    27901 gcctaccctt ccgaacacgc gggatttgcc ggtgcggcca cgtccatcct ttccggcacc
    27961 tttggttcgg aatttgccat gtccgataaa agccacgaat tgagaaccga attcaacggg
    28021 aagcccagga cctataaatc ctttgaagaa atggcgcagg aaaacgcctt gtccagattg
    28081 taccttgggg tcaattaccg aatggatacc gaggaaggtc tggacctggg aaaccgtgtt
    28141 gctgcgcggg ttgaaactct gccctggaaa aaatgatagt tggcatttga gtccacaagc
    28201 ggaccggatc ccccatacta actttctaac tttctaacta gctaactttc taactagcta
    28261 acttgcaaac taagttcacg catcccccgt ttccttcctc aatctttcca cctgggtcct
    28321 ccacagctgt ttttgttctt cgacttcatc cgcccaggca aaatccacga tttcaaggat
    28381 ggtttcatcc gtgatgtcgg cggtggtgat gcgaaattca aaatattcgt cttcatcttc
    28441 gctgtccagc caatggaacc ggagtctttc atcctcgatg tcgtcgatca gttcggcgac
    28501 ttccgtattg cctttccagg agaaggtgta atattccccg gtgatgtcca cggcgtcaca
    28561 aaaccaccgg accagggctg atggagtggt gatgaaatgg tagacaatgg tgggtgatgc
    28621 cttgagcagg aaatccacgg taaagagtac ccgtttcgtg ggctttccaa accgcatgag
    28681 cattttttct ttcttgacgg gagcggtcga aagcatgctg ggtttatgag tggggatcac
    28741 ctggaccagt ttgttaggtg tggctgtttt ccctgccttc gggcttttcg tcttggctgc
    28801 ttttcccggg gctccggctg tggcggcctg cgcaggtttg gaggatttgt cttttaccgc
    28861 ggggcttgct gccttcgccg agggtccggt ggttaggctt tttttggcgg gtttgagagc
    28921 cgcgggggcc ggctttgttt tggccgcttt cgcggatgca ctttcggttt tcacaggttt
    28981 tacagcgggc tttggcggtt cgggtttaag agatttattg gctttgggag cagtggcggg
    29041 ctttttgttg atggctgggg ctgttgttgc ctttcctttg gctggtttgc ctttcgtgcc
    29101 cggttttttc atactttatt ctgactgtct ataccctgca aagaaaaaca aatttttgaa
    29161 ttgtccattt aaaaaggaaa attttcattt tgaacttctt atgcatgata ttaaacaata
    29221 ttttaggaga atgccataat tactttatct ttgtgctcct gaatgactta tatatattca
    29281 gaatataatt ttgattatat taaattatta aacttttaat actgtaatga ggcagcttaa
    29341 gattacaaaa tctatcacca acagggaaag tcaatcactg gaaaaatatc tgcaggagat
    29401 agggaaagtg gacctgctga cgccggaaga cgaggtgacg cttgccaaac ttatcaaaca
    29461 gggagatcag gccgctctcg agaggctcac caaagccaat ctccggtttg tagtctccgt
    29521 tgcaaaacaa tatcaaaacc agggactttc cctcagtgac ctcataaatg aaggaaacct
    29581 ggggctgatc aaagcagccc aacgcttcga cgagacccgc ggattcaaat tcatttctta
    29641 cgcggtgtgg tggatccgtc aatccatcct ccaggctctg gctgaacaat cccgcatcgt
    29701 ccgccttccc ctcaacaaag tcgggtcgct caacaaaata aaccgcgctt tttccgaact
    29761 ggaacaacaa tttgaaagag aaccttcccc tgaagaactc gcttccatcc tggaaattcc
    29821 cagtgaagag gtggaaacca ccctgggcgt tgcggcacgc catgtgtcca tggacgcccc
    29881 cttcattgac ggcgaagaca ataccctatt ggatgtactg gaaaacaacg gcacgccatc
    29941 caccgacagc gccctggaat atcgcgaatc cctcaggaac gagatagaaa gatccctggg
    30001 tacgctcacg gaccgccagg ccgacgtcat caaactctat ttcggcatcg gggtcgaaca
    30061 tcccatgtcc ctggaagata tcggtgaaaa attcggcctt acccgcgaac gggtgcgcca
    30121 gatcaaggac aaggccatca ataaactgag gacggcttcc aggagcaaat tgctgaagca
    30181 ctacctgggc agctgatccc ttcgatcatt aggcccctat tactcgcgtg aatccggtat
    30241 cctgtggata ctgtgactcc tataaatcct accaatccta ttaatcctac caatcccacc
    30301 aatccttcca atcctattaa tcctacaaat cctacaaatc ctaccaatcc tattaatcct
    30361 attaatccta ccaatcctac caatcctacc aatcctacca atcctaccaa tcctattaat
    30421 cctaccaatc ctactaatcc tattaatcct accaatccta tcaaatttta ctcctcagtt
    30481 tcaccggtaa agtctttact tccccgtctc tcataatttt aaacatcaga gtctgtccca
    30541 cccgggattc atcccggata tcgaccagtt catcaaacgt agagatatcc cggccatcca
    30601 cctgaaggat gatatcatcc gggatgaggc ctccgtagtc ggctatgctg ccctcctgga
    30661 cactttttat caggaggccc tttttactgg gcagattttg ttccttggcc agttcctcat
    30721 cgatctcatg gatttcaatc cccaggctgc ccactcgttc cacgctgccg ttttggcgga
    30781 tttccgtgac gactttttgc atcaaattaa ccgggatcgc gaaggaataa ccggcaaaac
    30841 ttccgtccgg agagtagatc gccgtgttga tccctaccag tttgccggat acatccacca
    30901 gggcgcctcc cgaattgccg gggtttaccg cggcatcggt ctggataaat tcctcaatgg
    30961 gtttggaatc ctggatcagg ttgagtttat tccttccttt ggcgcttaca atgcccgcgg
    31021 tcaccgtaaa atccagatta aacggattgc cgacggccag cacccattcg cccactttca
    31081 gggcgtcgct gttgccgatc tctgccggcg taagtccgtt ggcatccacc ttcaggatag
    31141 ccagatcggt gcttggatca acccctattt tccgggccac aaatttgcgg tcatcaaaag
    31201 tcttcacatt gatttcatcg gcattggcga tgacgtggtt gttggtcacg atatatccat
    31261 cagcagtcag gatcacgccg ctgccctttc cttctctcgg tcccaggtca aactgaaagc
    31321 ccctgaacat tttttcaagg tcaaaaaatt catcaagccc ccttggggtc tggcgcctta
    31381 aattgtcctg cctgcgctgg ctggccaggg ggttggattt ggtttcgatg tacacgaccg
    31441 aacgtttggc tttatcggcc gccacagtaa aatcaaaggg tgccgccgga tttgccgccc
    31501 tagtccaggc gctgctcacc ggttggaggg agagtggatc ccccccgacg cccgtcttgg
    31561 acgttaataa caggaagccc cccaatgcga tcaccccgcc cataaggccg gctgataata
    31621 tctggaattt tacgttcata tcgatgttgg ttttgaggaa tacaaaatta actgcataaa
    31681 ataacagtat gatccgctcc tttttccgtt taacaatagt ttaaccggca acgggcaggc
    31741 cacccaccat tccccatcca ccaataactc cttagttttg cccgcctatt ctgaataaca
    31801 tgtcgagaaa aaaaaagctg gagcagtttg cggaattaga ccggtttgaa aacgtattgc
    31861 aatgcaccga tccgcaaagc agccaggcca taagatacga tggggaggtg gtggaaaccc
    31921 ggggtcactg gcgggacggg atttttaaaa acaataatcc cctggtcacc gagctcgcct
    31981 gtggaaaagg ggagtacacc gtagcccttg cccaaaggga tcccggaaaa aattttatcg
    32041 gaatagacat taaaggcaac cggatgcacc ggggggcgaa aaatagcctg gcgctcggcc
    32101 tgggcaatgc ccgtttcctt cgcatccgga tagaatggct cactcaccat ttcgcccccg
    32161 gcgaagtcga cgagatctgg atcactttcc cggatccgtt tttgaaaaaa agcaaggcaa
    32221 accgccggct cacctcgcct ggcttccaaa aaatgtacag gaacatcctt tccccgaccg
    32281 gcacggtcca tcttaagacg gacagcccgg aattatatga gttcacgctt gaaaccctta
    32341 tgtccacacc cggcgtaagc ataattgcac agtctgaaga tatagaccgg gatggcctga
    32401 acgaaggcat cctgtccatc caaacccatt atgagaaatt gcaccgcgct tccggtattt
    32461 ccatccggta tataagttgg atataccatg cgggggttga attgttgaat tgagaaattg
    32521 taatgccgct cccttcctcc cttcctccct tcctccctta cccctttcct cccttccttc
    32581 tgatcttccc gataatccaa agattgacaa aagcaaaccc ggacagggcc accatcatca
    32641 ctaaagaaat caggacctgg tctttcacca gacggatgat cagagggtgc aggagataac
    32701 ccatcaggct catgggaatg aggaccatgg ctttcagggc attggacagt ttaggattac
    32761 cagcgacctg gcgggccata aatttattca gggccagcac cacgagccaa agcccgatga
    32821 aggaataaaa catctattta aaatacaata aaatccgccg ggtcagtcgc ggtcataccg
    32881 gcagcaatcg tccaacttgg cataagcctc gtccggggct ttcacttttt cggtatcgta
    32941 tccgatggcg gccacccggt gatggatctc atccaggctg atgtgctccg gattgaaggt
    33001 gacggtaagc attttggtct ccttgtccca attgactctt ttcaccccct ttacccgaag
    33061 ggaagtttcg atttttttct tgcacatgcc gcaggagcct ttgaccatga acgtcgaggt
    33121 ctgatccgcc gccgaagcgg tgaatgcgga gagggagaaa accgcagtca acaaaagaag
    33181 gaaataaaaa ggctttttca tcctacaaag ttaacaggaa taccggcgtc aaagattgta
    33241 ccaggatctt agcattctgt taattttaca gcccatgatc caagaagaaa ccatcgatgc
    33301 ctgcatcgaa ctcgtagccg atagccagca ggactggctc cggctgatgt ccgaaaaaca
    33361 acccgtgctg gcctcttacc tgacctcgga agaccatgag gctttttcgg aaggagagac
    33421 ccagtacctg tattacctcg cggtcatgat ctggatgtgc tttgaccgca cctacccgga
    33481 aatggcggag gtcgatgaag aaaccctggc cctccgggaa gaacaaaact ggaaccgcgt
    33541 cgactccctc cgtccagctt cattaaagaa catcgttgac cggatcatgc cggactatcc
    33601 cgaacaggaa ttgttgtttt acctggaaga cgccctgcaa atggatgaag aagaccccga
    33661 acatccagtc acccgggcag gacaaatccc attgtttgtg accctgctga gtatttcgga
    33721 cgctttgagt gaagcggcga ccgcgggttg agcccggatc acccttcctt gcataagtac
    33781 tcctaaatcc gttcgacagt ttaacagttc ttcactccca aacccgggaa caacattccc
    33841 ccaatttata attctgctta tctttcccat ccgaaactta tgccgatgag ttcaaaaaca
    33901 tatctctttg ccctgaccgg acttttattc agtctgaccg ccagggctca ggatattcat
    33961 ttcaccatgt ttgacatgtc cccgctcacc ttgaatccgg ccctgatcgg ggatttccac
    34021 ggcaccttca ggattacggg caattaccgg attcaatggc cgaccatcgc caaatacaaa
    34081 acccccgcct ttggcataga tgcccctttg ttcaggggcc tgggcaaaaa cgactggatc
    34141 agttttggcc ttgcggcata ccaggataaa gccggtgatg aattcggcct caaaaaaacc
    34201 tcgggatgga tcggcggctc ttatcacctc gcgctcaaga aagacggatc ctccctgatt
    34261 tccatagggg tgcagagtgg tgggacctcc aggagctttg atggcatacc ccgctttcag
    34321 gatcccagtg atccggcatt ccccgggagc agcggaggcg gcaatgacaa taaaagaacc
    34381 tttgcggaca tcaatgcggg actgcttttc cggaacgctt tgaacaaaga aaaaacgtcc
    34441 tggctcaata tggggctctc cctctatcac ctttcccagc ccaacagcgc gcttggagga
    34501 gggggcggcg ggaccggcca gactcccggc caggacaaac agaatgtgct gatcaatgcc
    34561 catgcccaga tgatcatccc ccggggagac ctgctccagt ttgttccagg ggtggtattg
    34621 cattctgccg ggggcaactt tgaagtcgcc ggacaattga aaggcgaaat gctcatcaac
    34681 cgcgaaaagg atttttcggt gattggaggc ctgggttacc gggtaagcga cgcggccttt
    34741 gccattttcg gcgcccggtt caaggacctt aaagtggccc tggcttatga ctttaatatt
    34801 tccggagcca cctccagcac cagcgggata ggcggttatg aattgggggt cagctatatc
    34861 gccaagattt ataaaaagcc tgttgtcaaa ccggtgatct tctgcccgag attttgagag
    34921 ataggatgga gggttgagga ttgaggattg aagatctcaa ccctcaaccc tcaaccctca
    34981 accctcaacc tctaacctct gacctctgac caccattaat cgtccgattc cgtaaattcg
    35041 tctaataccg gtccggaaac ctttaatatt cttcattttt ggtcaacatt atataaaggc
    35101 tcatgtccat ccttactctt caacaggtca gtaaaacttt tcagaattat aaggccgtcg
    35161 accagatcag tttgaccgtc ccccgcggct gcatctatgg tttactcggt cccaacggag
    35221 ccggaaaaac cacgaccata cgcatgatca caggcatcac ccaccctgat tcgggggata
    35281 tttttctcgg cggcaaccgg gtcaataaaa ccatcccttc cagcattgga tacatgccgg
    35341 aagaaagggg cttgtataaa aaaatgagcg tctgggacca cctcctgtac ctggcccgtc
    35401 tcaaagggct ctccgtccag aaggcaaaag aggagctcaa attctggatc gaaaggctgg
    35461 atatgaaatc ctggtacacc aaaaaagtag aagagttgtc aaaaggcatg tcccagaaag
    35521 cccagttcat agcctccgtg atccacaaac ccgacctcct gatcctggat gaaccctttt
    35581 caggcctgga tcccatcaat accaacctga tcaaggatga gatatcccga ctgcgggaca
    35641 tgggaaccac tattttgttt tccacccacc ggatggaaca agtggaagaa atctgcgagc
    35701 gcatggtcct gatcaacaaa gggaaaaatg ttttggaggg agatgtcgaa gagatccgga
    35761 agtcccaccg gcaacacctc tttgaactgg tatccgaaaa tgatctgccc ggccggtttc
    35821 cggatgaatt tcacgaaatc cagcgcctgg gtaaaaaagt ccgcttcaac ccggaacccc
    35881 acatccagtc ggccgacctg ctccgttatt tcctgaatga aggcatagaa gtgaatgcct
    35941 tccgggaaca attccccagc atcaacgaca tttttattga aacggtagaa aaatccaacc
    36001 aggcatgaac aaactttggc tgatcatcaa gcgggaatac actacaaggg tattcaaaaa
    36061 atccttcatc ctgctcaccc tgcttgtacc cctgggcatc atagccttca gcgtaatcat
    36121 cggctttatc atgagccgca acacagtaca gaatatccga gtggcgatct cggatccgca
    36181 gcagatggtc aacgacagcc tgatcaggaa aaattcagaa attcagtata tcatcagtga
    36241 ccagcccttt gaatcgctta aatcaggcat tcagagtggc cagtaccagg ccgtcctgga
    36301 agtcatgccg ccggaggatc tcaaacaaaa ggattttaaa atcaggatca ataccgacga
    36361 acggctcgac atcaatacta cggaaagcat ccggaatacc ctggcccgcc aataccgcaa
    36421 ctacaaaata aaaaacctcc agctcaacat ggacgaaatc aacaccctgg atgtggatat
    36481 ctccctcgac cggcatgcgg tttttaacca ggataaaaaa cagtccaatg tttccaatgt
    36541 ggtgggtgcc atcctcgggg gcatcatggg catcctcatg tatatattcg tactaggaca
    36601 aggcatgatg gtcatgcgga gcgtcatgga agaaaaaatg acgcgcattg tagaagtcat
    36661 cctgtcatcg gtcaaaccca ttgaattgat gatgggcaaa atcattgggg tgggcgccgt
    36721 cggcctgacc cagctgctca tctggatcat cttcatgccc ctggccggca tcatcaccac
    36781 cctgatgctg ggcaaaaatg tttttaaacc ctcgatgtcc ggtgtcaatg tggaccaggc
    36841 cattcagcag gccgatagcc tgggcctgga gaatatcatg atggaactgg gccagatgaa
    36901 ctggtggttt atcatcccgg tattcctgct gttttttgtc ttcggctatt ttttatttgc
    36961 ctccctttgc gcggccatcg gcagcgcggt gggggacgac cagtccgaag cccagcaatt
    37021 gcttttaccc attaccctgc ttattgtcat gggcctctat attgccatgg cggccatccg
    37081 ggcgccttat agtaacctgg ccgtgatctc ctcattcatc ccgttttttg cacccatggt
    37141 gatgcccacc cggctggcct ttgatccgcc ggtctggcaa accctgctct ccctggccat
    37201 ccttgccata tcctgttatt tctttgcctt gcttgcggcc aagatctacc gcacaggcat
    37261 tctcatgtat gggaagaagg cgagttttaa ggagattggg aagtggttgt tttataagta
    37321 gaacagttgg taagttagca agttagtaag ttggcaagat gatcagtttg tcagttgatc
    37381 aattgtaatt ttgctccctt aacccttaac cttttaccgt taaccttttc agggtttccg
    37441 gtttataaat tccagtcgtt tgggtccggc gccgagtaca cgaaccgatt tccaatgggg
    37501 cggtaaagtc gttttaatct ggttcagccc cagttccgtg atttcaatcc ctgcaaaacc
    37561 catcaggaca ctttggagaa tgcctcccgc cccggtggca aaatagggat tggtaccccc
    37621 cttggtttcc gcgatgaccc ggaagggggg cagcaaatta ggctggtagg attcaacaaa
    37681 ccatttgtgc gcttcttcgc cacgcccgag gcgggaataa agggtggtaa aaacggcctg
    37741 ggtcatggcc ggcgtgcccg catccggaac gcgtacttta taatactcca ggtccataag
    37801 tatggctgcc gggtccgtga tttctttgag tggataggca agcaggttca catcggcctg
    37861 tttgatgcct tccccctggt aggatgcatg ttcccgggtg actccatcgg ggaatttcag
    37921 gatggggata ttcgaagcta cgtgcatcca gtccgggttg atatccagac ccagcaagcg
    37981 ggctgcctgg gtggcaaact gcagattggc tttagccgcc gcgttggtaa aggcattgtt
    38041 gtccacgttt tcggcccact cgtcggcggc gaccacgttt ttgatatcgt attgaccagg
    38101 gccgttacgt tcgacccggc tggcccagaa atccgcggtc tctttcagga tggggtatcc
    38161 cttttccctt agccattgtt tgtcctggct gacacaataa taattccagg ccgcaatgcc
    38221 cacacaggcg gtgatatgat gttcaaaagg cccgctcaaa gcccataccg gggtctcctc
    38281 caccccggtt gaggcgcttt cccagggata catggctccg cggaaccctt tgccgaaagc
    38341 atttcttttc gccgccccaa gcctttcaaa acggtactcc agcatgcttt cagccagggc
    38401 cggttgcatt accaggaggg cggggaacat ccaaagctcg gtatcccaga atacgtgccc
    38461 gttatacccc agtccgctca ggcccatggg agaaggggac agggaggttc cttcccgggt
    38521 aaaggcatac aaatgataca acatgctgtt gaggtcctgt tgggcctggt cgtccccttc
    38581 caccaggatc ctgctctgcc agagccggtc ccaggcctcc ttatgaaatt tcaataatct
    38641 ttctttgcct tccagcctgg cgtaaatggc gagtctttca gcttcattga gggcatcgtc
    38701 gtgatgcgcg gaggtgatgg aagccccggc aagggcaaaa cgataactcc cgccggctgc
    38761 aagttccttt gaaaacttca tcaggtgcat attattgtcc cacatttcat ggatggtccg
    38821 gggttccttt ccatggccct cttcaaacaa aaaagtattg gaagcgcaga ggtccatttt
    38881 gcccgtcggg cttttcgctt tagatgtcag caaaccgatc gtgacatggg gccggtcgat
    38941 ttcctggtaa taattttcaa cctcgcgcag ggcgtccggg gcttccatca cagaggctgc
    39001 agtcagtttg acggcacgca aagcggtgac ggaaacctcc atcatcacac aataaggcag
    39061 gtgccggagc gcggcatagg aataggtgat ccgggcctta tccatataat cgaaagaagc
    39121 cgaaaacagg gcatgtctca gatccatcga ctgattcatg ttccggatat tcccggcatc
    39181 cagccgctgg ccatcgattt cgagatacat attgaggagg ttgaaactcc ggatgaaatt
    39241 gctcaccctg ccacgcccgt aaagatcata ggctccggcc aggaccactt ccttcacctt
    39301 aaagggttcg ggtgaagaaa cgatgccgat cattccattg gcggaagtaa taccataata
    39361 atgggaggga tcgggggacg atacttttag atgccagggg tcgatggcct ggctggagac
    39421 atgggtggaa taattgaacc aaaggaggaa accacatatg atccgtttca taaaggaagg
    39481 ggtttaagga ataaccaaga taaactaatc cacgggcact cgaaaacgcc cgggatttga
    39541 cttccatccc ctgatttgga ctttctaaaa aacacagaag tattcctgtt tttcaaagga
    39601 ctgaatactc gaggtgataa ggctgtcaaa atcgaaggtc gtcaggggtg agataaggat
    39661 gaagaactta aatctttgta attcatgacc ttaaaaattt tcccggcagg acctttaaac
    39721 gttgcccttc ctggaatgca tgaaagcggt ggacagcctc ccgaaaaaat tccatgactt
    39781 gaagatccca aaagaatggt attatttttt cacaaaggag cattaatttg ataatataga
    39841 gcagccaaac cccaaacaaa atggaagaag catccctgat atccggatac cgtgtcgagt
    39901 ccaaggtctg ggatgaaatg tttgaccacc aggggatccg gcccctgtac cagaaaatgg
    39961 taaatgccat caaccggctc tcggcggagg aaatgaacca caaggatgac ctggccaaaa
    40021 aattgtttat gagccagggc gtcaccttta cggtttacag cagcggcgaa gggatagaaa
    40081 aaatatttcc tttcgacatc atcccgaggc tgatcggagc ggcggagtgg aaacacattg
    40141 aaaccggaat aaaacagcgg ctcaaagccc tcaacatttt cctccacgac atttatcacg
    40201 atcagtttat tgtacgggac ggcctgattc ccgttcagtt gatttattcc tgccccggtt
    40261 tcatccgggc catggtaggg gtcaaggtcc cgcaccagat ctatacccat atctcgggga
    40321 tcgacctgat ccgggaccag gacggacaat tctttgtgct ggaagacaac ctgcgcactc
    40381 cttccggcgt gtcctatatg atcgaaaacc ggagcatcac ttaccggatc ttccccgacc
    40441 tcctgccccg gatcggggtg aggccggtga aggactaccc ggatctgttg ctcaacaacc
    40501 tgttatccct gtccaaccgg caaaccagcg ctcccaccgt ggtcctgctc acgccgggca
    40561 tattcaattc ggcctatttc gagcacacca ccctggccag gctgatgggc atagaactgg
    40621 tggaaggcaa agacctggta gccgacggca acaagattta catgaaaacg atcaatggcc
    40681 tgaagcaggt ggacgtcatc taccgcaggg tggatgatga attcatagat cccctggttt
    40741 tccgaaggga tagcctgctc ggggtgccgg gcctgtattc cgcttaccgg gccggccagg
    40801 tcgccgtggt gaatgccatc ggcaacggcg tggccgacga caaagccatc tattcctatg
    40861 tcccggaaat gatccggtac tacctgaatg aggagccggt ccttaaaaat gtgcccacct
    40921 atcccatgga aaacccggaa ctgagggcca tggtcttcaa tgacctgtcc cggatggtca
    40981 tcaaaaagac caacgaatcg ggcggttacg gcatgctcat cggcagcagc gccactgaaa
    41041 aacaaatggc cgaattccgc aaggctattg aactggatcc gagaagtttc atcgcccaac
    41101 ccatcatcaa cctgtcctcc gtcccttgtt atatccaggg caggctccag gcccgccgcg
    41161 tggacctgcg gccctacgcc ctgtatggcc cgcatggcat cgatatcgta ccgggaggcc
    41221 tcacccgggt cgcgctgaaa gaaggttcct tggtggtcaa ttcgtcgcag ggagggggga
    41281 gtaaggatac ttgggtttgt aatgaatgat taaaagaatt aacaattaag aattaagaat
    41341 taaggatgct gagccgggtt gctgaatcga ttttctggat ggccaggtat atggagagga
    41401 ccaatggcac cctcaggctg gtgcgcacca gctatatttc ttcacaggat gaaatacatg
    41461 attttacctg ggcccccatc ctgcagcatt acagcgatcg cagtgatttt ccgcctgggg
    41521 attcgaagca agtgattgaa cacctgctgc tggacaagga aaatgcttcc tccgtcatca
    41581 acaacatcac ccgggcacgt gaaaacgcgc gggccgtcca ggatcacatc accaaagaag
    41641 tctggcaatg cctcaatgat ttttaccatc tcatccggaa cgaacggatc catgcacaga
    41701 tcctccgggg cgacgcgatc tccgcgatgg acgccctgat tgaaaatggc tggctgtacc
    41761 acggcgtact ggacacgacc ctggcccggg gcaatggctt tgctttttta tctctcggca
    41821 aattcataga gcgggccatt ctttcggcta acaacctttc catcaaactg gcggagctgg
    41881 attaccacct cgaccgtccc ctggaaatac cggccttgcg ttatcttttg tattcgctgt
    41941 ccggttatga attgtatcta aaaacccacc gcggcaacct caatgccgac ctggtcatcc
    42001 agcaaatcct ttaccacaac cattttccgc actccttgct gtattgcctg gtccgcatcg
    42061 ggcgttatct ggaacgcctg cagccggaaa gcctgccgga agtgtacaaa aacctggatt
    42121 ttctgaccgg aaaattgatg aatcacctca aatacacccc cgtggacatc cgcaaaggga
    42181 aagaactcgg ccggttcctg gaccatattc gcagcggcct gtatgaaatc acggaggcca
    42241 tgaacaaaaa ttatttcgga cacttttaag cccttgtcaa taccatgacc agatatcagc
    42301 tgaaacacat tacgcgatac ctttatcctt caccggtaag ggaaagcatc aaccagatca
    42361 tgctttatcc catagaggat gccctccagg atgtaaaaag gcatgaaatc gcgatcagcc
    42421 atcagcccgc catcgaaatc ttttccgatt attttggcaa ccgtacaggc attttttcca
    42481 tccctcaacc cctttccgaa ctccagatcc agtcgaccgc cgatgtggtg gtgcatgaat
    42541 ctcttttgcc ggccgatgat caagccgcat caaagcaatg gtccgaactc cgggcgttcg
    42601 gttccctcgc ttcctatatc gattttttat ggcaggaaaa atccgaccgc ctggatgacc
    42661 tggtcgccat taccaaagac ctcgccgatc cggcaaaaac ccccttaaca gttgcaaagg
    42721 atatgtctgg ctttgtttac cggcattttg aatataaaaa agggatcacc gatacggaaa
    42781 ccggggtgca cgagatctgg tccatgcggg ccggggtctg ccaggatttt gcccacgtcc
    42841 tgctcgccat gctccgcctg tgcggcatcc ccgcccgcta tgtgagcggt tatatctgtc
    42901 ctaaaaatca tgaattccgc ggcgaagggg ccacccatgc ctgggttgaa gcatttatcc
    42961 cattcgaagg ctggcttggc ctcgacccta ccaacaactg catcgtgagc gatcggcaca
    43021 tccgccttgc ggtcggtaga aatttcagcg atgtcactcc tgtaaaggga acctacaagg
    43081 gctcctccga gcaccggctc gaagtgggtg tcgaaatcat caatggccat tcgtcagacc
    43141 ggccggtaat atccgatgcc cctcccgtca ttacctatca ggcccaccag gcaagccaga
    43201 atgaaaattc ctatcgccgt gcgatggaga tgcagcagca gcagtagtca gtagtcaata
    43261 gtcagtagtc aatagtcaat agtcaatagt cagtagtcag taatcaggtg tcagttgtaa
    43321 ttttggtcct ttagccccta accattcacc catctttatt tagggcagtg gtctgtaata
    43381 agtggtcagt ttggaagttg cgctcccttg acctttgacc cttgcccctt gacctcagac
    43441 ttctgacctc tcacctctaa cctttatctt tgtccctcct ttctaataaa taaccgtcat
    43501 gaaaaaaaga caatttatca aaagcgtcgg cgccgtggga cttttttcca tccttcacaa
    43561 aaatccgctg aaggctgaaa ccgaagtacc ggaattcctc aatccaatga ttcccccggc
    43621 ggatcagtac accctgccgc cgctggatta cgctttcggc gccctggagc cgcatattga
    43681 tgcaaagacc atggaaatcc atcatgaccg ccatcatgcg gcctatgtca ccaatctcaa
    43741 caaggaactc agtacccatg cagcggcggc ggcccttccc cttgaaaagt tatttgccca
    43801 gagtaaaaaa tattcagcgg gcatccgcaa taacggcggc ggccattaca atcattccct
    43861 gttttggaaa ttgctgaagc ctgctgcctc atccacaccc atcggtgcgc tcgcaaccgc
    43921 catcaatgca aaatttaaag acctggccaa tttcaaggac ctgatgaata aagccggcac
    43981 cggccgtttt ggttccggat gggcctggct ggtactggat aataaaaata acctggtcat
    44041 cggctccacc cccaaccagg ataatcccct gatggatgtg gccgaggtaa aaggatatcc
    44101 cctccttggc atcgacgtct gggaacatgc ctattacctc aaatatcaaa acaaaagggc
    44161 agactacctc agcgccatct ggaatgtcat caactgggat gaagtggcta aaaggtatga
    44221 ggagggaatg aagaggaata agaagaaagg ttgaggttaa ggttgaggtt gaggttaagg
    44281 ttgagactga ggtccatcca ccatccacca tccaccatcc accacccacc acccaccacc
    44341 tacttaattc cttctattta tttcttttac agatatatac aacttctata atactt
//
LOCUS       DAOQXT010000029        44145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_962_length_44145_cov_6.097573, whole genome shotgun sequence.
ACCESSION   DAOQXT010000029 DAOQXT010000000
VERSION     DAOQXT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 44145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44145
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_962_length_44145_cov_6.097573"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKM27_06980"
     CDS             complement(<1..221)
                     /locus_tag="PKM27_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNR07040.1"
                     /translation="MKKELLERLNRMKVEALAGGGESRIRQQHEKGKLTARERIDLLL
                     DPHSFEEIGMLVTHRNTDFDMQNALYPGDG"
     gene            337..1548
                     /locus_tag="PKM27_06985"
     CDS             337..1548
                     /locus_tag="PKM27_06985"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNR07041.1"
                     /translation="MSSTAIESVLSDRVMSMTESATLQMAGMARKMKEQGIDVITLSL
                     GEPDFDTPQHIKDAALKAMEAGHTKYTAVAGNLDLREAICAKLKRENGLDFKPSQIVV
                     SNGAKQDIANLCLAMINPGDEVIILSPYWVSYSDIVKMCGGEAVVLSAGVEEDFKVPA
                     SRVEAAISDRTKLIMFSSPCNPTGSVFNEDELKAIAEVVARHENLYIMSDEIYEHINF
                     TGKHISIASFPEVKDRTIVVNGFSKAFAMTGWRLGYMAAPQFIADACVKIQGQMTSGA
                     NSIAQKAGVAALNSDLRPTQEMTMAFRRRRDLIISLLKEIPGFKCNFPTGAFYIFPDI
                     SAYFGKSDGVTTIHNSDDFSLFLLNQAHVATVGGSAFGDDHCFRISYAASEDQIREAV
                     RRIKEAVSKLK"
     gene            1617..2693
                     /locus_tag="PKM27_06990"
     CDS             1617..2693
                     /locus_tag="PKM27_06990"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNR07042.1"
                     /translation="MAAIKFISNNTQFFPVLRKRVNQYFEEKGIPQTGNYKLYIKTAL
                     LSAGLIGTYLWLVFFTPHSILLGLLLCGILGVLVAAVGFNVMHDGAHGSYSNISWLNN
                     VMAYSLNVLGGSSFMWKHKHNIAHHSFTNIEGMDEDIDIKPFLRVNTSQKRYWFHRFQ
                     HIYGLILYGFTYLMWIFFNDFKKYFSRKVTDHTALPPMNLREHLTFWLTKFAYAVTFF
                     ILPVWQVGLLKTLLGYGMLVFITGILIAIVFQLAHVHEEAAFVEPQGEPFVIENDWAI
                     HQINTTANFATKQKWHSWFWGGLNYQIEHHLFPRISHVHYPEISKIVKQTCREFNIAY
                     NEFPTVIHAIHSHFTHLKKLGVAY"
     gene            complement(2726..3136)
                     /locus_tag="PKM27_06995"
     CDS             complement(2726..3136)
                     /locus_tag="PKM27_06995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNR07043.1"
                     /translation="MDKARLQEELNFHHYAMIRPSPVHGIGVFAVRPIPKGCRSMFSK
                     EPGEWVRLSFEEVAALPEASRYMIETYCLFDEQDYFVPAGGFKTMDIALYLNHSDEPN
                     IQSIDEGAEFEALRDIQPGEELFIDYGTIVNSEE"
     gene            3442..4410
                     /locus_tag="PKM27_07000"
     CDS             3442..4410
                     /locus_tag="PKM27_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNR07044.1"
                     /translation="MILSIFLCLIGFAILILGAHLLVKGASSAAKRKGISNLAIGLTV
                     VAFGTSMPEFTVSMLAALDGKNDASFGNVIGSNSFNLLFILGVSGLIYPLAVHRNTVN
                     KEIPISLGAAILLWLVVNDSWHGWSQGGMNILSRIDSLVFLVFFCGFLYYVYLSMKEA
                     KDKNIEDGYHLYSTGIAILMILGGLAFLIGGGKMVVNSSVKIAQYYGWSEKLIGLTIL
                     AAGTSLPELATSAVAAYHKHDDIAIGNVIGSNIFNILFILGFTGLVHPIPYNAEMNKD
                     IYVLIAGTVALMVFLFVFRKHKLDRWKSFLMLAAYILYTVYLIRLG"
     gene            complement(4534..6453)
                     /gene="dnaK"
                     /locus_tag="PKM27_07005"
     CDS             complement(4534..6453)
                     /gene="dnaK"
                     /locus_tag="PKM27_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNR07045.1"
                     /translation="MGKIIGIDLGTTNSCVSVIEGNEPTVIANDEGRRTTPSIVAFMD
                     NGERKVGDPAKRQAITNPKRTISSIKRFMGRRFAEVTEETKRLSYLVKRGDNDTVRVE
                     IDGRAYTPQEISAIVLQKMKKTAEDFLGQEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LKVKRIINEPTAAALAYGLDKKHKDMTIAVYDLGGGTFDISILDLGDGVFEVKSTNGD
                     THLGGDDFDAVIIDWLAEEFKKQEAIDLRKDPMALQRLKDAAERAKIELSSSMETSIT
                     LPYVTAVDGVPKHLDVKLSRAKFEQLVDHLVARTIKPCEQALKDAGLSKNEIDEVILV
                     GGSTRIPKIQQAVEDFFGKKPSKGVNPDEVVAIGAAIQGGVLTGEVKDVLLLDVTPLS
                     MGIETMGGVFDTVIEGNTTIPSKKSKVYSTAADNQPSVEIHILQGERAMAKDNRTVGR
                     FILDGIPPAPRGVPQIEVIFDMDANGILNVTAKDKGTGKEQKVRIEASTGLSKDEIER
                     MKREAEANADADKNAKETIEKINMADSLIFQTEKQLKEYGDKLSAMNKANIEGALLEL
                     KEAHKAKDVASIEKATEKLNNVWQAASQELYQAQQQAGAGADTAGSAGGSQQTGSANG
                     DNVTDVEFEEVADKK"
     gene            complement(6587..7774)
                     /locus_tag="PKM27_07010"
     CDS             complement(6587..7774)
                     /locus_tag="PKM27_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR07046.1"
                     /translation="MTLLVPDRVFDGERMHEGWKVLVREDKILEAGPVIDWHPGRMDT
                     VNLPGITLLPGLIEGHAHILLHPYNETSWNDQVLRESLAERVLRASNHLFASLSKGFT
                     TLRDLGTEGADYADVGIKQALGKQVISGPDLLVAGRAIVASGSYGPKGFVFKPPQGAE
                     EADAFDLVRVVRNQIGQGADLIKIYADYRWGPFGQAMPSFSQEEIRTIVETAKSSGRV
                     VVAHATTKEGMRRAVLGGVSTIEHGDEGDLEIFRLMKEHRVSYCPTLAAGEAILQYAG
                     WKKDTDPLPDKILKKRQSFREALSAGVHILFGGDVGVFPHGDNARELILMGEYGMDPE
                     AVLRSATSGNADALGLKNRGRIQHGLLADLVGIRGNPIENLQDIYHVDFVLKNGKRVV
                     KSH"
     gene            complement(7963..8184)
                     /locus_tag="PKM27_07015"
     CDS             complement(7963..8184)
                     /locus_tag="PKM27_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07047.1"
                     /translation="MKSIKITGTVEYIDLGTGFWAIITPDGKKYRPIHMPDQLKTKGR
                     QVSCRVVPAEEDLSIFMWGTPVRITVFET"
     gene            complement(8207..9031)
                     /locus_tag="PKM27_07020"
     CDS             complement(8207..9031)
                     /locus_tag="PKM27_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNR07048.1"
                     /translation="MKTVSERFAECIELLKTKGKINSFRQFSLSIDYLPETLAQIIND
                     RKEPGQEVLKKASEIYDLNLTYLLTGKGSIFNMEDYPRHELRILTVVTDAHNEERIVH
                     VPVPAQAGYVSDLADPVFYSELPTYSLPDFRYSQGTHRSFDVSGKSMEPVLEEGDKVV
                     CSYLEPSQWMQGIKDDQVYVIVTWTDVVVKRVKNLLRQQQALCLQSDNPGYQSYMLAA
                     ADIREVWYVRARISAFCHVPYANPLAPAVASLQETVREQQEMIRFLKQTIEKIEKV"
     gene            complement(9217..9975)
                     /gene="tpiA"
                     /locus_tag="PKM27_07025"
     CDS             complement(9217..9975)
                     /gene="tpiA"
                     /locus_tag="PKM27_07025"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNR07049.1"
                     /translation="MKRDKIVAGNWKMNLGLDEGMELVEKLAKAYTHHEPRMVVIPPF
                     LHALAVQNYLQSVHSPIEVGVQNCHQETKGAFTGEISASMIRSCRIPFVVLGHSERRQ
                     YFAETDALLAQKVKAALGQGLIPIYCVGEPLTVREGSGQNDFVAHQLEKGLFHLSPEQ
                     FKEVVIAYEPIWAIGTGVTASPEQAQDMHAFIRHRIAIHYSEAVAAATRILYGGSVKG
                     SNARDLFVQPDVDGALVGGASLDADDFLRIASSI"
     gene            10076..12139
                     /gene="ppk1"
                     /locus_tag="PKM27_07030"
     CDS             10076..12139
                     /gene="ppk1"
                     /locus_tag="PKM27_07030"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNR07050.1"
                     /translation="MKPSNLPLIERDLSWLSFNHRVLQEAKDPTVPLLDRLKFMAIYS
                     SNLEEFFRVRVAQIMNLLRVGRGTRKALSFDPERLIKSIMKVVNQHQQELTTLFNKQL
                     LPELRFNGIHILNPEMLNAEQLDFVDNYFKESLQAFVQPILLVDSKIKPFLNSGALYL
                     YLHLLDRSKNKKKDHFAIVRVPSDQLPRFVILPETKKGEHQVMILDDVVRLCVKYIFP
                     GFILKDSYSIKLTRDSELYIDDETQGDLVAKIKKSLAKRNVGPASRLVYDRNMPGTML
                     NLLKKTFDMHDVDVIPEGKYHNNFDFFKLPDFGKKYLKEPALPQLPYPALEESEDLFS
                     EIRKRDHMIHVPYHKYESVIRFFEEASKDSAVTHIKVVQYRVARESRIMEALIDAARE
                     GKKVSVFIEVKARFDEEANLRWGEKLEKAGVLVKYSFPELKVHSKIAWIRRMENKKAK
                     QYAYLSTGNFNEDTALVYSDLGLFTADGRLTHEVKKIFDYLESGQIPTAPFKHLLVGK
                     NNLREELSNLLDFEISQAKAGKPAEIIIKLNSLEDKDIIRHLYQCSRAGVKVKLIIRG
                     ICCIQPSKKKLSENIEAISIVDRYLEHARIFLFHHGGREKIYCSSADWMERNLSFRIE
                     TAFPVYDPKIREQIKHLLYLQWKDNVKARDLAHQNTNPYRRNGSSISIRSQLETYSYF
                     KNSAK"
     gene            12173..13507
                     /gene="purB"
                     /locus_tag="PKM27_07035"
     CDS             12173..13507
                     /gene="purB"
                     /locus_tag="PKM27_07035"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNR07051.1"
                     /translation="MITALSPIDGRYHSLCAPLGEYFSEYALIKNRVYIEIEYFRALT
                     GLGLPPLAGAEKHLDLLSDLFARFNETEALRIKEIEKVTNHDVKAVEYYLREKIKNSP
                     LEPYSEFIHFGLTSQDINNTAQPFALHRSNQEFLFPLAEEVIRAMDGKAHQWKDQAML
                     AHTHGQPASPTTLGKEIKVFVERLRGQFALLRDIPVTAKFGGATGNFNAHQVSFPGID
                     WHGFADHFVSGLGLDRIRWTTQIEPYDYLAAYCHAWCRINTILLDFCRDLWMYISMNY
                     LSQKVIRDEVGSSAMPHKVNPIDFENAEGNLGIANALFTHLAGKLPISRLQRDLTDST
                     VLRNLGVPLAHTTIAFKSILKGLTKIEANNLALHDALGKSWVVVAEGIQTVLRREGIK
                     GSYELLKELTRGRDEISESDFREFIAQLPVPLHIKEELLQITPFNYTGDTEF"
     gene            13558..15036
                     /gene="crtI"
                     /locus_tag="PKM27_07040"
     CDS             13558..15036
                     /gene="crtI"
                     /locus_tag="PKM27_07040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNR07052.1"
                     /translation="MQKTIAVIGAGFAGMSAAALLAKQGFSVTLLEKNQGPGGRASLL
                     EEAGFKFDKGPSFYWMPDIFERFFQQFNKSASDYYELIRLDPSYKVFWKDREPISMPA
                     RLEELTGLFESLERGAGLQLNRFLAEAKKKYDTGMGRFVYLPSLSLTEYLSFDLFRQS
                     LKLDLFSSVAGHIAHYFKNPYLRQLLEFPVLFLGAKPSATPALYSMMNYADLVLGSWY
                     PSGGMYEVSKAFFQTGKDQGVRFLFNQEVLSIRALPDKRMKLVTRGGFDQDFDYIVSG
                     ADYHHTETSLLQEKDRSYPQSYWNSRTMAPSALLYFLGFDRRIDQLEHHSLFFDADFD
                     QHAREIYDRPGWPADPLFYTNSSSLTDARCAPAGHHNLVALIPVSVELKDNEKVRQRY
                     LDLILQRLTHHTGIDARKHLVYQKSYAQADFIDEYHSLKGNAYGLANTLLQTGPFRPK
                     IRSKKLPNLFYCGQLTVPGPGLPPSIISGEIVAKYIHQITKP"
     gene            15033..15878
                     /locus_tag="PKM27_07045"
     CDS             15033..15878
                     /locus_tag="PKM27_07045"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNR07053.1"
                     /translation="MSRELFDQVSQSCSKLVTQAYSTSFSIGIRLLHPTVRPHIYALY
                     GFVRLADEIVDTFHDYDKKELLDQFVAQTEEALEKKISLNPILHQFQRTVNLYHIDRT
                     LIEAFLHSMKMDLDQKSHTRESYEEYILGSAEVVGLMCLHVFCEGDKTLYARLAPHAQ
                     RLGAAFQKVNFLRDIQSDQSGLGRHYFPHWKEGQAFSEEIKEQIISEIKSDFKAGREG
                     IVQLPGTCRTGVYAAYVYYRALLIRIARTPSSRLFQSRIRINNVLKFLLLLYAGLRIK
                     WQGIK"
     gene            15875..16333
                     /locus_tag="PKM27_07050"
     CDS             15875..16333
                     /locus_tag="PKM27_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNR07054.1"
                     /translation="MKVYTKTWQQDLPVRLEPAWSFFSRPENLQAITPDFMNFRILTD
                     IQGREMYEGMMIHYTVSPLLGLSMRWTTEITHCKPMNYFIDEQRSGPFALWHHEHHFQ
                     EIPAGVRMTDRLTYGLGYGWLGELLDQFVIGSRIDAIFAYREEVLKLKFK"
     gene            16330..16797
                     /locus_tag="PKM27_07055"
     CDS             16330..16797
                     /locus_tag="PKM27_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNR07055.1"
                     /translation="MMANVLITIATFCAMEFVAWSAHRYLMHGLLWSWHRDHHDPKAR
                     QHSFFETNDRFFLVFAIPSFLSFVAGLLSPYPFFTYIGLGILIYGICYFLVHDVFIHQ
                     RFKWFRNSNWIYFRAIRKAHKVHHKHLGKEDGECFGMLWVPLKYFKEAAASKV"
     gene            complement(16794..17420)
                     /locus_tag="PKM27_07060"
     CDS             complement(16794..17420)
                     /locus_tag="PKM27_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HNR07056.1"
                     /translation="MTEHDLSEKAQRDKEVDSVIRNHLVWSMGAGFIPVPVADFFAVS
                     AIQLDMVRQLCRVYDIEFKETEGKAIISSITSSGLARLAARTVVKFIPGAGSFIGGMA
                     VAVLSGASTYALGQAFRKHFETGGTFLDFDLSRLKKIYDEMFEKGKDVAEQIRKEEKQ
                     TGEHMIQVSHGATAGIIDKLSELGKMKDQGLITEDEFLKMKEKLLSEI"
     gene            17577..18290
                     /locus_tag="PKM27_07065"
     CDS             17577..18290
                     /locus_tag="PKM27_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNR07057.1"
                     /translation="MQITVHRADTRGQVDFGWLQSKHTFSFGHYYDPERVHFGALRVI
                     NDDTVAGGKGFGMHHHDNMEIISIPLSGDLEHKDSMGNQTVIRSGDIQVMSAGTGIHH
                     SEYNKNKDLPVKFLQIWVLPDKRNVIPRYDQVTLDPASMNNRLTQVLSPDAGDAGVWI
                     HQQAWFHLGRFDKAFTQEYSLKKKGNGVYLFILSGDLILNGQTYHERDGLGISGTDVV
                     KISSTGPAEFLLMEVPLIS"
     gene            18359..19066
                     /locus_tag="PKM27_07070"
     CDS             18359..19066
                     /locus_tag="PKM27_07070"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNR07058.1"
                     /translation="MEKYLYLFLDLASFSIPFLFSFEKKWIHFIGRWKAIAAGLGAMA
                     LFFLVWDYAFTKSGVWGFNDRYITGIKAYGLPLEEFLFFILIGYACLFIYESLHHLAP
                     RPAWGKTAKTLFALIAIVNLVVAVLHTDRLYTFTALALNAVGLFLVLLFFKAFPWSKF
                     LLGYAVSLIPFSLVNGVLTGGFTEEPVVWYNNTENLGIRMGTIPLEDSQYMMLMMLIS
                     TGLYEMVLRRNSLPKQA"
     gene            complement(19094..25117)
                     /locus_tag="PKM27_07075"
     CDS             complement(19094..25117)
                     /locus_tag="PKM27_07075"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNR07059.1"
                     /translation="MRLQFAGWVTAVLFLLHLQCASSKNKQDTMALRSDYKTLWRQID
                     SLEKEGLPESARTKTLLVYEKAKKENEEINAIKSLIYLAKYSAGLEESGLLAAIHLIE
                     SDLDLGSRTGQSIKHSLLAELYQGYFRSNIWVIRDRTPVLSDTLDAGSPETWSAVDFL
                     NKIRKHHLQAMEHPGLKNVPVSNYLGLLTEGEESDSLRPFLYDILAHRALDYFMQGMS
                     DLDLAGDEYRFDDPRLFGPTSQFLEINPQYRQEDPVFYVQVIHLFQTLEEFHQKDSNP
                     AAYLDVVQKRIQYVYEHFSGEGKDALYETNLDQWMALYPQHQGIFYLLKANLYIQWGN
                     RYEPFGEDSVYRRWLTEARSILEQVIAKYPGSREAIQAGNQIKALVQAQVQGQVEQVN
                     VPGQPFRLFVQYRNTPRIHGRVIKVPDTLRKKLQEDRRPENIQLLLQASPLQNFQQAL
                     PSVEDLQEHRTELKIDALPPGMYGILLSNHADFQIGKGVIALMYTHVSNLAYLHEAGY
                     QARLTVGRDFTKTLFIVDRTHGTPQEGVQAVFYEQSYNDNRRTQEWKEVQRLVSDKSG
                     AVSPALPTNKNYSVALIKGKDSLWLQDGFPHFSYDQNGLKEREDALLFLDRSLYRPGQ
                     LLYFKGLALIRDAEDQPRIQKNRKVTVSLQDANGQLVSKQVFVSSDFGSFHGSFVLPA
                     GGLSGSMSLVTNLNQASVNFQVEEYKRPRFELTFDTAATLYRLNEPVLVRGHARNFAG
                     NAVDNAMVRYTVTRERWIRPLPWWSWKIYFPQRSNPVIIAKGEAQTDVSGQFQVPFIA
                     LPEANMGTDQDQSFIFSVQVDITDFNGETRSGTTSMRIGKKNLVLQSELGTYLPADKS
                     GSFEIKATDLNDIPVQASGTWTLDQLQQPTTRYRKRYWPRPDQQVITESEYRTWFPSD
                     LYWNEDDLTTWPSSKRIQSSSFQTGQASRLNQTLEPGAYKLVALARDRYQQETEWVHY
                     FWVYDLADKNLPSWKALQMLQDSTALEPGQVLNYHIQFQKNPQHVLFTHSRESDARWI
                     TLDSLTTETVSIGEADRGKIFYLRGYAVRDNRFYVEQLQYHIPWSDKILRIETRSFRD
                     QLLPGQKETWSLNISGQAKDKFLAEVVASMYDQSLDEYYPHGWNTGLFYPRDIQGRPY
                     SAPSFTGVPSETVSMEPLDAEELKPIRYRNLDWFGFELGYYSFFRSRAMGIETKVLQN
                     DAMVPEAAQAAESKKEGPVPPGSTEPSQAPQANAIVTPRKNLNETVFFYPDILTDQEG
                     NFSLRFTMNEALTKWKLMIFGHTRDLKSGYLEKSITTKKDLMVFPNAPRFVRQGDVLI
                     LPAKISNQSGKAIQGRARLELFDPITGNDLRNAFGLSPAEIAFEAAPAQSASVGWTIK
                     VPGAYLGMLGYRVWAESGTYTDGEENILPVLSNRMLVTETLPVQVEANQSKSFSFDAL
                     RASASGTLRHHAYTLEMTTNPAWYAIQALPYLMEYPYDCSEQVVNRMYANLLGAKIMN
                     SNPAIRSIFEAWAAKDVLKSPLQKNEELKNAVLAETPWVKDALSEQEQQKLIALFFDS
                     NRLQAEKDKGIRILQERQLSNGGFPWFTGRDDWYISQYILEILGQMKAAGLLDSSLDE
                     MLSRLIRYCDEELQRYYEEQKRLAGKNEVGLSPIAVHYLYARSLFKETAIPSGTQSAF
                     GYFLKSAENQWLKQNLGSQAMIALASHRWTPKGIVPGKIIASLKERAQHHAELGMYWK
                     FDAGYRWFELPIETQVLLIEAFDRIANDRASVEKMKLWLLKNKQTNHWPSTKSTAAAV
                     NALVHFGNDWIPETRVPEISIGGEPIKPGPDQVIPGVGYFKIIKTAEQVKPSMAALQI
                     HNENNHIVWGSAYWQYFEDLDKIKTTGKLPLQMNKSLYLEATEGSKKVLKPFEQLSPG
                     DKVLVRIELKADRPMDYVHLKDMRASGFEPVGVISEYKYQGGLFYFESTKDLATHFFF
                     DHISPGTYVLEYALRVSQKGDFSNGISSIQCLYAPEFSSHSAGTRVVVR"
     gene            complement(25243..25752)
                     /locus_tag="PKM27_07080"
     CDS             complement(25243..25752)
                     /locus_tag="PKM27_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNR07060.1"
                     /translation="MIRKILIGLLFVLVVIQFIRPAKNQGNDQSKHINTLYPMPDDVK
                     QILALACDDCHSNNTRYPWYNNFQPIAWWLADHVKDGKRHLNFSELAGRRTALQYHKM
                     EEVVEMVDEGEMPLKSYTLTHGDAKLSTDQRKKIIAWAQSVMDAMKMKYPADSLVMPE
                     RPSGPPPGN"
     gene            complement(25781..26815)
                     /locus_tag="PKM27_07085"
     CDS             complement(25781..26815)
                     /locus_tag="PKM27_07085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNR07061.1"
                     /translation="MKTDFLIVGQGLAGTLTGHALERMGASVVIMGLEKPHAASSLAA
                     GIIHPVSGRKFVKAWLYDQLIAQCHETYEEIERNLKVPLFRKLDMHLYVSSVKEENDL
                     LSQSRRYTYDDLLRPLTVNGWDMQNSRRGYAINAYQLDIQGLLGHSANRWKAKQRYLP
                     EHMEYPALSREGDSWRYKDVVAGQVIFCEGVWCRGNPWFSHLPVIPNKGQVLTLDPAG
                     FQNIDYLVKSGLLLTRWKEVIWAGATYEWDFDYPGPDSYGWNYLYRRLENLLSQPYRV
                     RSHMAGLRPTVPDRKPILAEHAELPGLWAVNGFGTKGSSLGPYVTRHLADLLNGREKI
                     NRFSPSRFKE"
     gene            complement(26805..27158)
                     /locus_tag="PKM27_07090"
     CDS             complement(26805..27158)
                     /locus_tag="PKM27_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNR07062.1"
                     /translation="MGLDGIRAYCLDKPGVTESLPFGPDALVFKAGKKIFAILALSSE
                     RPWVNLKCEPDYALELREGHPEIIAGYHMNKRHWNTIYLDGFLKSDLLKKLIDLSYEL
                     VLHPRRGKKEASGEN"
     gene            complement(27158..28003)
                     /locus_tag="PKM27_07095"
     CDS             complement(27158..28003)
                     /locus_tag="PKM27_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR07063.1"
                     /translation="MEVQDFRKEVVDKSYEVPVVVDFWAPWCGPCRALGPVIESLAQE
                     ANGTWVLAKLNTDQHQDIAQALNIMSIPAVKMIYQGKIIGEFVGALPKPQVQAWLAKH
                     LPDMDALAGQEGGTEGEEEAGMKLPDPDEILDKITQVPDPEFVMYLDEYLGQNPDSDK
                     AKVYLASHLVFSDPAEAEALTQHIKEGNPLFDRVQDVRTLATFFVHQFDDSKPGKLLT
                     EARDLIDHSQPEKGMEKIIESLQTDKSYMDETARKSGIALFHIYGKNHPVTKSLRKYF
                     DMAIY"
     gene            complement(28027..28959)
                     /locus_tag="PKM27_07100"
     CDS             complement(28027..28959)
                     /locus_tag="PKM27_07100"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNR07064.1"
                     /translation="MGEQAQFSTKHLIGIKYVTTKDIELIFHTADEFKEVINRPIKRV
                     PTLRDVTIANIFFENSTRTRISFELAEKRLSADVINFSASSSSVSKGETLLDTVNNIL
                     SMKVDMVVIRHPAAGVPVYLANRVSANIINAGDGTHEHPTQALLDAYSLIEKLGPLSG
                     KKIAIIGDILHSRVALSNIFCLNKLGARVRVCGPPTLIPKHIASLGVEVSHDVESTLR
                     WCDAANVLRIQLERQEIPYLPSLREYAQYYGINAAMLDRIDKPILLMHPGPINRGVEL
                     SSDAADSKQSIILQQVENGVAIRMAVLYLLANRN"
     gene            complement(28963..29514)
                     /gene="pyrR"
                     /locus_tag="PKM27_07105"
     CDS             complement(28963..29514)
                     /gene="pyrR"
                     /locus_tag="PKM27_07105"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNR07065.1"
                     /translation="MERGRIILKSPRFNLTLQRLAHQLIENYNHFENTCLVGIQPRGV
                     ELSDRIYTLITEINPGHKARYGKLDITFYRDDFRLRKALKANETEMDFVVEDQRVVLI
                     DDVLYTGRTIQAALTALNHFGRPKEVELLVLIDRRFNREVPVSADYIGYTVDAVDEAH
                     VVVDWDPDPDKNEVRLYKKKQED"
     gene            complement(29554..30342)
                     /locus_tag="PKM27_07110"
     CDS             complement(29554..30342)
                     /locus_tag="PKM27_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNR07066.1"
                     /translation="MKGIFTTVLLILSLAAGFSCKVTTEGKHEKPMWIYILAGQSNMA
                     GRGLIEAEDTVSHPGVLYLDSTNQWALAREPLHYYEPARTGLDCGLSFAKEMLLLNQA
                     SIRIGLIPCAVGGSSAEHWLYDSLYRGVRLYTNFERKVDIAKKDGEIKGILWHQGESN
                     ANAAALSSYSMILDSLFLKMRQTCGHADLPILMGELARFLSWNQFHGFHIPVNELLLK
                     KTRESPHLFLVSSEGLESKGDSIHFNSASLRELGRRYARALMQQ"
     gene            complement(30349..31089)
                     /gene="madM"
                     /locus_tag="PKM27_07115"
     CDS             complement(30349..31089)
                     /gene="madM"
                     /locus_tag="PKM27_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadM"
                     /protein_id="HNR07067.1"
                     /translation="MIPQILIKNGLITAFLSVGILILIAELISKKVFRNKIPTVALAI
                     FMALMAAFIAGGPNGAADIPIMSELALLGGTMLRDFTVVATAMNASLDEIRKAGWLGF
                     VSIFLGLILTFFSGAACALLFGYRDAVSLATIGAGACTFIVGPVTGTALGASSEVIAI
                     SIAAGVVKSIAVTIGTPLVAKWIGLNNPQTAMIYGGMLGTTSGVAAGLAATDPRLVPY
                     GALTATFYTGMGCLLCPSLFFLVLNLYF"
     gene            complement(31086..31448)
                     /locus_tag="PKM27_07120"
     CDS             complement(31086..31448)
                     /locus_tag="PKM27_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadL"
                     /protein_id="HNR07068.1"
                     /translation="MTIYGVALLAFCYLAGLWMGEGLASLMKVKSNVGGVGFGMILLI
                     LFQSWPLTKKWLVREVPSGISFWSRMYIPVVVAMSAIQNARMAFSSGYLAVLAGILPT
                     AFCFILIPFLASRFNTSR"
     gene            complement(31445..32938)
                     /locus_tag="PKM27_07125"
     CDS             complement(31445..32938)
                     /locus_tag="PKM27_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNR07069.1"
                     /translation="MTFIIQQAIQFKGRVAIREGIKAFTYDELILRAGKVATFLLRGK
                     DDLGSARIAYMVNPGIAYVAVQWGIWQAGGVAVPLSLNHPEEAVRYYLRDSGAEVVIC
                     SPEYVKSLQPLASGMQLNLTDTEKVLEDQDLKPFFPRIEGQRDAMILYTSGTTDKPKG
                     VVSTHANIRSQITTLVEAWEWSSEDQIISFLPLHHVHGIINVVGCALWSGACVHFMPE
                     FDPSGVFHLILEENLTLFMAVPTIYYKLIQYFNRLDPEAKQKLRDRFGRFRLMVSGSA
                     ALPVSVLDQWKVITGHTLLERYGMTEIGMAISNPLHGARKAGYVGMPLPGVGVRLMDE
                     EGKPVREGQPGEIQIKGPNVFKEYWQKPAASRESFTSDGWFRTGDIALVEHGYFRILG
                     RNSVDIIKSGGYKISALEIEEALRAIEGVKDTAVVGIADEEWGELIAAAIQSPHLGEE
                     DIPAIKESLLKKLPGYKMPRLYRFVAELPRNAMGKVVKQEVKTIFTA"
     gene            complement(32967..36530)
                     /locus_tag="PKM27_07130"
     CDS             complement(32967..36530)
                     /locus_tag="PKM27_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNR07070.1"
                     /translation="MKWIPFGLAGFLMFLACRTRTDLFTPIRPESSGLTFVNEITEYD
                     TFNILNTEFIYNGAGVAIGDLNGDGLEDLYFAGNQVGNRCYLNLGNLKFKDITEAAGV
                     QKREGQWSSGVNILDVNADGKQDIYVCNTLIRNKDQRKNLLFINQGNQGEIPVFKESA
                     EEYGLDDTSHASHAQWLDYDRDGDLDLFIGTNFIDRPRPGTFVKDVHNDCALNCDKLY
                     RNDPDPVSGHPRFTEISHEAGLNLHGYSHSSLIYDFNEDGWPDIYVANDYLSDDLIYI
                     NNRDGTFSNRLADIFRHLASSAMGSDLGDINNDGRMEVVTAEMLPNTNLRKKTLLGPT
                     NYSYYLYIQEYGYQYQFTRNVLQLNNGLVPETGLPSFSEIGFLSGVHETEWSWAPLFA
                     DFDRDGYEDLFITNGFPKDVTDHDFGEYRAEASNLVSDMDLQSLIPIVKIPNFLFRNK
                     GDLRFEDITGASGLSIRSFSNGAAYADLDLDGDLDLVVHNIDDPPFLFKNQTEELDRK
                     TNTTHFLQVRLKGPASNPDAIGAKVNVYTGKGMQSRLVLSGRGYLSQPSRTLYFGLGE
                     ATLVDSISIEWPDGSRQIAGNIQADQFLTYGFEGQPFSGQLAPSPEGLFSMEKPASLG
                     LDYKHEEKDFIDFNIQKTVPHKMSQFGTPLAVGDLNGDGLDDLILGGSANHQEMVYLQ
                     DGSGKFNKKALQSKKESDADAEDASLVLFDADGDTDLDLVIARGSYENLMSDASVFQV
                     LLLINDGKGNFSPDTLAIPKTARTCASVARAADIDQDGDLDLFIGGRVLPGYYPKADK
                     SFILLNEAAGGKARFSLAPDAWNAGLGQAGIVNDALWSDYDNDGRPDLLLATEWGPVT
                     IYRNDGKSLSRLSAPALDRHMGWWTGLLAGDFDNDGDMDYVAGNYGKNIYFTCSANEP
                     MTIYAKDFDSNGSLDPFISCYWRDTLGVKREYFYHGRDDMIKQLILIRRKFQTYTSFG
                     LATVNQVFTPKELEGAMILKANNMNSVLLVNDGKGDFRVDTLPVEAQFAPLYGMVAQD
                     INGDEFLDLVLTGNDYGIELTQGRADAMQGLVLINDTHGGFKSLPFQESGIYIPGDSK
                     ALVRLSMGAGGRLIAMRNLDSLAVAAFKAPGMTFTFQPGEYSGMMEFSSGARRKIEWG
                     AGAAVNGQSSSKQVSGPGVSRITLYHMKSKSERVITPASLH"
     gene            complement(36748..38490)
                     /locus_tag="PKM27_07135"
     CDS             complement(36748..38490)
                     /locus_tag="PKM27_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR07071.1"
                     /translation="MKTDFNWRKVSLSLTAMLLFTFACKDNFLEVPPTGSLGNAQLAT
                     KAGVEGALIGAYSMLNSKGYTQVAAPNNWVFGSILGGEANKGTDPGDFSSINPIQRYE
                     TEPTSGDVNGTWLAKYEGISRCNAVLRLAAQATDISAADLKRIQGETRFLRGHYYFEL
                     KKIFNSVPYIDETVDYGTGIEAVPNSPNFWDKIEADFKFAYDNLPETHSQAGRANKWA
                     AGSYLGKAYLFQKKWNDAKAIFDAVIANGKTTNGKKYGLVANYAQIFNAANDNHEESV
                     FAVQSSNNSGSVNNSNHFDVLNYPYNTGTDGPGNCCGFFQPSFELANSFRTDANGLPL
                     LDGSYNSAANALKHDYNIESSAPFTPDAGNLDPRLDHSVGRRSIPYLDWIAHPGKNWI
                     RSQAYAGPYSNKKYVYYKTQENTLTDGSGWTRGYATMNYTIIRFADVLLMAAEAEAEG
                     GSLEKAREYVNLVRARAANPASWVKKPDGSNAANYVIGLYNTPWTDKAAALNAIRFER
                     KLELSGEGHRFFDLVRWGIAKTELNKYLANEAVLLVTMFGGATFRDGREFLPIPQTQI
                     DIQGSDVLTQNPSY"
     gene            complement(38523..41762)
                     /locus_tag="PKM27_07140"
     CDS             complement(38523..41762)
                     /locus_tag="PKM27_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNR07072.1"
                     /translation="MKRENFTPKLKKAFRSFLSAGLLLLTVSVSAQVTGVVTDRATGE
                     PLIGASILIKGTTTGTVTDVDGSYSIAAKEGDVFQVSYTGFTAIEVTVGATPVLNIAL
                     DEGVLIDEVVVTGYTTTKKKDFSGAVSLVKAAELKAVPSGNVEQQLQGRVSGVTVVTN
                     GQPGTTNIIRVRGFGALGGNQPLYIVDGQPVGSTDFLNPDDIESTTVLKDAASASIYG
                     ARAANGVIVYTTKKGSKNSRKVNITYDGLFGFTDPNVAGAPKMLSPQEQADWTHIAYR
                     NNAAATGTEVKYNHPQYGTQAQATLPDYLHANGANGVRGSVDVEAIKAAAAANPGTVF
                     LIKPNLAGTDWYDEITRTAGLMRHSLGFSGGGENSRFYVSLGAQNQDGILLENKFKRY
                     SLRANMEYNLTNRLRFGQNVQFTYRSVVGQEGGSGGIGIADDESTVLSAYRMPSVIPV
                     FDELGQYASTRAAGFNNPRNPVRSRVLNTQNDRTFNMNGLGNLYLELDVIKGLVLRSS
                     VGGDFGSNFFKNYNYRYLGDSEPEASNSFSEGSQNSFGWVNTNTATYNFKVGNHGFSV
                     LAGAEFLNTGKGRFINGSGLNPFSTDLDYVNLNVVQSPTVNSGLFSGVNFESLFGKVD
                     YNLNDKYYISATVRRDGSSRFGINNRYGVFPAFAAAWRVTGEDFLRANEVLTDLKIRG
                     GWGQMGNSNNVDPANQYSLYAANRGNSFYPIEGQNSGVNEGYYRSRIGNPDAKWETST
                     TINVGFDATLFDGKWEIVGDLWKKKTDDLLYTVPLPGVVGSFAAAPAVNIAKMNNSGF
                     DLLVINRGKISSNWNYDVSWTSSFLKNEITYLAPNVTFFGGNAYRGVSPIRNAVGQPL
                     SSFFGYKVLGYFKDAADVANSPAQVGKGVGRFKYEDINGDGKIDPTDRTFIGNPVPKY
                     TGGLVFNASWKNLTIGTYLYTSLGNKIFNFSKWFTDFFGSFEGSGKGERAKESWTPAL
                     GDKAKAPIWESASNVSTNAGENSWYVEDGSYLRMQYLSIGYALPTSLIKPLGLSKVNL
                     TLAGTNIFTITKYQGLDPGVGGDVDTNFGVDVGNYPVTRGMTLGINVGF"
     gene            42271..42612
                     /locus_tag="PKM27_07145"
     CDS             42271..42612
                     /locus_tag="PKM27_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07073.1"
                     /translation="MNIEEINRKHIIKTDCLYRVGYGLKSRLLDFQNGIIYLEIVFGS
                     RWQNNYSATVYELAHCWKDNNPELRHAQGCKVFIIDGRKDPEKIDLHHAGIRVDYDAR
                     KGILFAPFTLN"
     gene            42914..>44145
                     /locus_tag="PKM27_07150"
     CDS             42914..>44145
                     /locus_tag="PKM27_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR07074.1"
                     /translation="MEFNSAAIEAKWKRFWDENQTYKVSNTSTRPKYYVLDMFPYPSG
                     SGLHVGHPLGYIASDIFARYKRMRGFEVLHPMGFDSFGLPAEQYAIQTGIHPAVSTAR
                     NIARYREQLGNIGFSYDWSRQVSTSDPAFYRWTQWIFIQLFHHWYDLEQEKARPIGDL
                     EKIFTESGNLLVQASHSQVPQFSAGEWKSYSEKTKSDLLMNYRLAYRKEAFVNWCEAL
                     GTVLANDEIKDGLSERGGHPVERKPMTQWFLRITAYAERLLQDLHEVEFSESMKIMQS
                     NWIGRSTGAQLFFELEGHPDKLEIYTTRADTIFGATFMVLAPEHELVGRITTGEQEQA
                     VADYLRYVNSRSDRDRMSEVKEVTGVFTGAYAINPFNGVRIPVWIAEYVLKEYGTGAI
                     MAVPADDARDMAFAKKFEL"
BASE COUNT        10702 a        11442 c        10716 g        11285 t
ORIGIN      
        1 ccatctccgg ggtaaagcgc attttgcatg tcaaaatccg tgttccggtg ggtcaccagc
       61 atgccaattt cctcaaaaga atgagggtcg agcagcaaat cgatcctttc cctggcggtg
      121 agtttgcctt tttcatgttg ctgtcgaatg cgggattcac cacccccggc cagggcttca
      181 actttcatcc ggttcagcct ttccaataat tcttttttca tgatttcatg cgtagccttt
      241 gaaatggtga ttttttaaat attgtaatga acaggcagga tgactgaatt acttttcaat
      301 atattattaa ttttgtgatt caactttaaa aattatatgt cttcaactgc gattgaatcc
      361 gtcttaagcg accgggtaat gtccatgacc gaatccgcca cccttcaaat ggccggtatg
      421 gcccggaaaa tgaaggaaca gggcatcgat gtgatcaccc tcagcctggg agagccggat
      481 ttcgacaccc cccaacacat caaagatgcc gcccttaaag ccatggaagc cggacatact
      541 aaatacactg ccgttgcagg taacctcgac ctccgggaag cgatttgcgc gaaactgaaa
      601 agggaaaacg gtttggattt caagcccagc cagatcgtag tatccaacgg ggcgaagcag
      661 gatatcgcca atttatgcct ggccatgatc aacccagggg atgaagtgat catcctgtct
      721 ccctactggg tctcctactc agacatcgta aaaatgtgcg gaggggaagc cgtggtcttg
      781 tctgcaggag tggaggaaga ttttaaagta cccgcttccc gggtggaagc agcgatttcg
      841 gaccggacca aactgattat gttctccagc ccctgcaacc ccaccggttc ggtgttcaac
      901 gaggatgaac tcaaagccat cgcagaagtc gtagcccggc atgaaaacct ctatatcatg
      961 tccgatgaga tctatgaaca tatcaatttc acgggcaaac acatcagcat tgcctctttc
     1021 cccgaagtca aggaccgcac cattgtagtc aatggtttct ccaaggcctt tgccatgacc
     1081 ggctggaggc tggggtacat ggcggcgccc cagttcattg cggatgcctg tgttaaaatc
     1141 cagggccaga tgacctccgg agcgaattcc attgcccaaa aagcaggggt ggcagcacta
     1201 aattcggacc tcaggcctac ccaggaaatg accatggcct tcaggcgccg gagggatctc
     1261 ataatatccc tcctcaagga aattcccggc tttaagtgta attttccaac cggggccttt
     1321 tatatttttc ccgatatcag cgcttacttc ggtaaaagcg acggggtgac caccatccac
     1381 aattcggacg atttttccct ctttttactc aaccaggccc atgtcgccac cgtcggtggc
     1441 tccgccttcg gggatgacca ttgtttccgg atttcctacg cggcgagtga agaccagatc
     1501 cgcgaggcag tacggcgtat taaagaggcg gtttcaaagc tgaaataacc ttaattttgc
     1561 acctcctgtt gtgaagcatt ttttgttaaa cttcaaatca agctattcca ttccatatgg
     1621 ctgccatcaa atttatcagt aacaacacgc aatttttccc tgttctcaga aagagggtaa
     1681 accagtattt tgaagaaaaa ggcatccccc agaccgggaa ttacaaatta tacattaaaa
     1741 ccgccctgct ttcggcgggt ctgatcggca cttatttgtg gctggtcttc tttaccccgc
     1801 attccatcct cctgggcctc ctgctgtgcg ggatccttgg ggtcctggtg gccgcggtgg
     1861 gcttcaacgt catgcacgac ggcgcccatg gaagttattc caatataagc tggctgaata
     1921 atgttatggc ctattccctg aatgtactgg gcgggtcctc cttcatgtgg aaacacaaac
     1981 acaatattgc ccaccattcc tttaccaaca tcgaaggcat ggacgaagac attgatatca
     2041 aacccttcct gagggtcaat acttcccaaa aaagatactg gttccaccgc tttcagcata
     2101 tttacggctt gatcctctat ggttttactt acctgatgtg gatttttttc aatgacttca
     2161 aaaaatattt cagccgcaaa gtgactgacc atacggcgct gccacccatg aatttaaggg
     2221 aacatctcac tttttggttg accaagttcg cctatgccgt cactttcttc atcctcccgg
     2281 tctggcaggt cgggctgctt aaaaccctgc tgggttatgg catgctggta tttattacag
     2341 gcattctgat cgcgatcgtc ttccagttgg cccatgtgca tgaagaagca gcatttgtcg
     2401 agccccaggg agaacctttt gtcattgaaa acgactgggc catccatcag atcaatacca
     2461 ccgccaattt tgccaccaaa caaaaatggc atagttggtt ttggggcggt ttgaattacc
     2521 agatcgaaca tcacctcttt cccaggatca gccatgtgca ttatcctgaa atcagcaaga
     2581 tcgtcaaaca aacctgcagg gaattcaata tagcctataa tgaatttccg accgtcatcc
     2641 atgccatcca ttcccacttt acccatctga agaaactggg tgtggcctat tgatccaggc
     2701 agcggaaggt ggtatatgga ttgacctatt cttcggaatt cacgatcgta ccatagtcaa
     2761 taaacagttc ctccccgggc tggatgtccc gcagggcctc aaactccgcc ccctcatcaa
     2821 tggattggat attgggctca tcggaatgat tgagatacaa ggcgatgtcc atggttttaa
     2881 accctcctgc aggaacaaaa taatcctgtt cgtcaaacag gcaataggtt tcaatcatat
     2941 accttgaagc ttccgggagg gcggccactt cctcaaaaga aagacggacc cattctccgg
     3001 gctccttgga aaacatggaa cggcaacctt tgggtatggg cctgaccgca aataccccaa
     3061 tgccatggac cggggaaggc ctgatcatgg cgtaatgatg aaaattgagc tcttcctgta
     3121 atctggcttt atccatactt gttccaaaaa ttatagggcc ctaaaattat tgaagtcccg
     3181 gcatgtttct gaagctattt tgggctatag atttatttta tagtctggca cttcctcatt
     3241 ccgccctggc gatccctgtt tctctgggta cgaacagttg gaggttcccc ctcccccatt
     3301 accccatcct cccattctct cagcgcccgg cactctggca cctactttcc atatttaccg
     3361 gggccctggg aaaggagggt attaataatt ttctcctttg aaaccggatg gaggggcgga
     3421 tgggaaaatt atatttgtcc catgattttg agcatttttc tctgtcttat tggctttgcc
     3481 atactcattc tgggggctca tcttttggta aagggtgcct cgtctgcagc aaaacggaag
     3541 ggaatatcca acctggccat tggactaacc gtggttgcct tcgggacctc gatgcccgag
     3601 tttaccgtga gtatgttggc tgctttggat gggaagaatg acgcttcctt tggtaatgtc
     3661 atagggagca acagtttcaa tctgctgttt atcctgggtg tttcaggcct gatatacccc
     3721 ctggccgttc accgcaacac cgtcaacaag gaaattccga tatccctggg tgcagccatc
     3781 ctgctctggc tcgtggtcaa tgattcgtgg cacggctgga gccaaggcgg aatgaatata
     3841 ttatccagaa tagattcact ggttttcctg gtgtttttct gtggtttcct ttattatgtt
     3901 tatttaagca tgaaggaagc aaaagataaa aacatcgaag acggatacca tttatacagt
     3961 acgggaattg ccatactcat gatcctcggt ggcctggcct ttttaatcgg aggcgggaaa
     4021 atggttgtga acagctcggt taaaatcgcc caatattacg ggtggagtga aaaactgatt
     4081 ggcctcacga ttctggctgc agggacttct ctcccggaac tggcgacttc tgcggtcgcc
     4141 gcatatcaca aacacgatga tatcgcaatt ggcaacgtca tcggttcaaa tatctttaat
     4201 atccttttta tcctgggatt taccggactt gtacatccca ttccttacaa tgccgaaatg
     4261 aacaaggata tttatgtcct gattgcggga acagtggccc ttatggtttt cctcttcgtc
     4321 ttccggaagc ataaactgga ccggtggaaa tcgtttttaa tgctggccgc ttatatcctg
     4381 tacaccgttt atctaatcag attaggctag gttccggagg atcagggatg ttccccaaat
     4441 aatgcgctcc gccgaaggga gaaatggaac gcatacaaac aaaaaaggga ccccgaagat
     4501 cgaggtccct gcatgtcgct ggaagccgaa taattatttt ttatcggcca cttcttcaaa
     4561 ctctacatcg gtcacgttgt ccccattggc agaacccgtt tgctggcttc ctcccgccga
     4621 accggccgta tccgccccgg ctccggcctg ctgctgtgcc tggtaaagtt cctggctggc
     4681 ggcttgccaa acattgttaa gtttttccgt tgccttttca atgctggcca cgtccttggc
     4741 tttgtgggct tccttgagtt ccaggagggc cccttcaata ttcgccttgt tcatggcgga
     4801 cagcttgtcg ccgtactctt tgagctgttt ttcagtctgg aagatgaggg aatccgccat
     4861 attgattttc tcaatcgtct ctttggcatt cttgtcggcg tctgcattgg cttccgcctc
     4921 ccgtttcatc cgttcaatct catccttgga aaggccggtg gaggcttcga tccttacttt
     4981 ctgttccttt ccggtgcctt tgtcttttgc cgttacattg agaatgccgt tggcatccat
     5041 atcgaaaatc acttcgatct ggggcacgcc tcgcggagca ggaggtatcc cgtcgaggat
     5101 aaaccgtcct acggtcctgt tgtctttggc catggcccgt tcaccctgga ggatgtggat
     5161 ttcaacggaa ggctgattat cggccgcagt ggaatacact tttgattttt tggaaggaat
     5221 ggtggtattt ccttcaatga ccgtgtcaaa aactccaccc atggtttcaa tgcccatgga
     5281 caggggggtg acatcaagca gcaatacatc cttgacttcc ccggtcaaca ccccgccctg
     5341 gatggcggcg cctatggcaa ccacctcgtc cgggttgacg cctttgctgg gttttttacc
     5401 aaaaaagtct tccaccgcct gttgaatttt ggggatgcgg gtgctgccac cgaccaaaat
     5461 cacttcatca atttcatttt ttgacaggcc ggcgtcttta agagcctgtt cgcaaggctt
     5521 gatggtccgt gctacgagat gatccaccaa ctgttcgaat tttgcacggc tgagtttgac
     5581 atccaggtgt tttggcaccc catctaccgc tgttacataa ggcagggtga tggaggtttc
     5641 catcgaggag gacaattcaa tctttgccct ttccgcagca tctttaagcc tttgcagggc
     5701 catgggatcc ttgcgaagat caatggcttc ctgtttttta aactcttcgg ccagccagtc
     5761 aatgatcacc gcatcaaaat catccccacc aaggtgggta tccccattgg tggacttgac
     5821 ttcaaatacg ccgtcaccca ggtccagaat ggaaatatca aaggtcccac cgcccaggtc
     5881 gtaaacggca atggtcatgt ctttgtgttt tttatccaga ccatacgcca gggcggcagc
     5941 ggtcggttca ttgatgatcc tttttacctt gaggcccgcg atctccccgg cctctttggt
     6001 ggcctggcgc tgagaatcat taaaatatgc cggaacggtg atcacggctt cggttacttc
     6061 ctggcccagg aaatcctcgg ccgttttttt cattttctga agtacgatgg ccgatatctc
     6121 ctggggagta taagccctgc cgtcgatttc caccctcacg gtatcgttgt ccccccgttt
     6181 taccaggtag gatagacgtt tggtttcttc ggtaacctcg gcaaacctcc ttcccatgaa
     6241 gcgtttgatg gaggatatgg tgcgttttgg attggtgatg gcctgtcttt ttgcagggtc
     6301 tcccactttg cgttccccat tgtccataaa agcaacgatg gaaggggtcg tcctccttcc
     6361 ttcatcattg gctatgacag tgggttcatt tccttcaatg acggatacgc acgagttcgt
     6421 ggtacccaag tcgattccta ttatttttcc catttttatt tgactttatt aatctgaaac
     6481 tcattaatca atagatatgc caaggccaat ttttgccaaa acaggtgatt taatccaata
     6541 aaattgaggg aaatgtcagt tgaatgattg gattactgcc aaaaagtcag tggctcttaa
     6601 ctaccctctt gccgtttttc agcacaaaat caacatgata aatatcctgt aaattttcta
     6661 tgggattgcc ccggatgccg acgaggtcgg cgaggaggcc atgttgaatc cggccccggt
     6721 ttttaagacc cagggcatcg gcattccctg aggtggcgct gcgcagtacc gcttccggat
     6781 ccatcccgta ttctcccatc aggatcagtt cccgggcatt gtctccgtgc ggaaaaaccc
     6841 cgacatcccc tccgaaaagt atatggaccc ctgcggatag ggcttcgcgg aaactttggc
     6901 gctttttcag gatcttatcg ggcaaagggt ctgtatcttt tttccatccg gcgtattgca
     6961 atatggcttc accggccgcc agggtcggac aataggaaac ccggtgttct ttcatcaatc
     7021 tgaatatttc caagtccccc tcgtcgccat gctcaatggt gcttactcct cccaggaccg
     7081 ccctgcgcat cccttctttt gttgtggcat gggccaccac taccctgccg ctgctcttgg
     7141 ccgtttccac aatggtccga atctcttcct gcgaaaaaga aggcatggct tgcccaaatg
     7201 ggccccagcg gtaatcggca taaatcttta ttaaatcagc cccctggccg atctgattac
     7261 ggaccacccg gaccagatcg aaggcatctg cctcttccgc tccttgcggg ggtttaaaaa
     7321 caaaaccctt cggtccatag ctcccggaag ccacaatggc cctaccggcc accagcaaat
     7381 cgggtccgga gatgacctgt ttgcctaagg cttgtttaat tcccacatcg gcataatctg
     7441 caccttccgt acccaaatcc cggagggtgg taaagccttt gcttaaacta gcaaataaat
     7501 gatttgaagc tctcaagacc cgttccgcca gggattcccg gagtacctgg tcattccaac
     7561 tggtttcatt atagggatgc agcaggatgt gggcatgtcc ttcaatcagg cccggcagga
     7621 gggttattcc gggcaggttt accgtatcca tccggccagg atgccaatct atgacagggc
     7681 ccgcctccag gatcttgtcc tccctgacca gaactttcca gccctcatgc atgcgctctc
     7741 cgtcaaatac ccggtcaggt accagaaggg tcatttgagc cggcaggaat ttgattaaaa
     7801 acaaaccggt taaggaaagg aaaagcctag ctgcacacag agccatgtca caatttaaaa
     7861 attaaaggcc ttgatcggaa aagtattata ggcgggacga gggggggggc agcacaaaat
     7921 gattcgtccc cgggcttgag cctttaaaaa tccggaaagg tttcaggttt caaacacggt
     7981 gatcctgacc ggagtgcccc acataaaaat ggacaggtcc tcctccgccg gcactacccg
     8041 gcagctcacc tgcctgccct ttgttttcaa ctggtcgggc atatgaatgg gtctgtattt
     8101 tttgccatcc ggggtgatga tggcccaaaa tccggtaccc agatcaatat actcaaccgt
     8161 tccggttatc ttgatggact tcatcgtaca aatgaggagg atttattcag actttttcaa
     8221 ttttttcgat ggtttgtttt aaaaaccgga tcatttcctg ctgttccctg accgtttcct
     8281 ggagggaggc aaccgccgga gcaagcggat tggcataagg gacatggcaa aacgcgctga
     8341 tccttgccct cacataccag acttcacgaa tgtctgccgc agccagcata taggattgat
     8401 agccgggatt gtcggattgc aaacaaagtg cttgttgttg ccggagaaga ttttttacac
     8461 gctttaccac tacatcggtc caggtcacga tcacataaac ctggtcatcc ttgatgccct
     8521 gcatccattg gctgggttcg agatagctgc ataccacctt gtccccttct tccaggacag
     8581 gctccatgct tttgccggag acatcaaaag accgatgggt accctggcta tacctgaagt
     8641 caggaaggga atatgtaggc aattcactat aaaaaacagg atcagccagg tcactcacgt
     8701 aaccggcttg ggcaggtacc gggacatgaa cgatccgctc ttcattgtgc gcatcggtca
     8761 ccaccgtgag gatcctgagt tcatgcctgg gatagtcttc catattaaat attgatccct
     8821 tgccggtaag caggtaggta aggttcagat cgtagatttc actggctttt ttcaagactt
     8881 cctgcccggg ctctttccgg tcattgatga tctgggccag cgtttccgga agataatcga
     8941 tggataagga aaactgacgg aaggaattga tctttccctt ggtcttcaac agttcaatac
     9001 actccgcaaa tcgttccgaa accgttttca tattaaaaaa aagtgaaata tgttttcggc
     9061 tcaaatataa gcaaaaggaa gatttgacca aataaaatgt aaaatatttt ccgcgtgcct
     9121 aatgtcagcc agaactaatg aaacggccgg cagaagaccg gaagtttacg gatctgcccg
     9181 gagggtcgta tcgttcagga agaatccggt tttccgtcaa atcgaagagg cgatgcggag
     9241 aaagtcatcg gcgtcgaggg aagccccccc tacgagggcc ccgtccacgt caggctgtac
     9301 aaacaggtct ctggcgttgg acccttttac gcttcctcca tagagtatcc gtgtagcggc
     9361 tgcgactgcc tcgctgtaat gaatggctat tcgatggcgg atgaaggcat gcatgtcctg
     9421 ggcctgttca gggcttgccg tgaccccggt accaatggcc cagatgggtt cataggcgat
     9481 caccacttct ttaaactgtt ccggactaag gtgaaataag cctttttcca gttggtgggc
     9541 tacaaaatca ttttgacctg atccttccct gaccgtcagg ggctcaccca cacaataaat
     9601 ggggatcagg ccctggccga gggccgcttt tactttttga gccagaagag catccgtttc
     9661 ggcgaaatat tgcctgcgtt cggagtgccc gagaaccacg aaaggaatcc tgcaggaacg
     9721 gatcatggac gcagagattt ctccggtgaa ggcacccttg gtttcctggt ggcaattttg
     9781 cacacccact tcgatgggcg aatggacgga ttgaaggtaa ttctgcacag ccagggcatg
     9841 cagaaagggt ggaatgacca ccatccgggg ttcgtgatgg gtgtacgctt tggccagttt
     9901 ttcgaccagt tccatccctt cgtcgagtcc caggttcatc ttccagttgc cggcaacgat
     9961 tttatcgcgc ttcatatttt tttaatttcg gcaaaggtaa aaaagtgggg ctattcccat
    10021 accgcagttt ggaatatcga agcggtacgt ttactttgta gccactgcaa taaacatgaa
    10081 gccctcaaac cttcccctga tagaacggga tcttagctgg ttgtccttca accaccgtgt
    10141 gctccaggaa gcgaaagacc cgaccgttcc tcttttggat cgtttgaagt ttatggctat
    10201 ttattcttcc aacctggaag aatttttccg ggtccgggtg gcccagatca tgaacctgct
    10261 gcgggtggga aggggtacgc gcaaagcgct ttccttcgat cctgagcggc tgatcaagtc
    10321 gatcatgaag gtagtcaacc aacaccagca ggaactcacc acccttttta acaaacaatt
    10381 gctgccggaa ctccggttta atggtattca tatcctgaat ccggagatgc tgaatgctga
    10441 gcaactggat tttgtagaca attattttaa agagagcctg caggccttcg ttcaacccat
    10501 cctcctggtg gacagcaaga tcaaaccttt cctgaacagc ggggccctct atctgtatct
    10561 ccatttgctg gaccgctcaa aaaataaaaa gaaagatcat tttgccatcg tgcgcgtgcc
    10621 ttccgaccag ttaccgcggt tcgtcatcct ccccgaaacc aaaaaagggg agcaccaggt
    10681 gatgatcctc gatgatgtgg tccggctctg tgtaaaatat atttttccag gctttatcct
    10741 aaaagacagt tattccataa aactgacccg ggattccgaa ctgtacatcg acgatgagac
    10801 ccagggcgac ctggtagcca aaatcaaaaa aagcctggca aaaagaaatg tggggccggc
    10861 ctccaggctg gtctatgacc gcaacatgcc ggggaccatg ctcaacctgc tgaaaaagac
    10921 ctttgacatg catgacgtgg atgtgatccc ggagggaaaa tatcacaata attttgactt
    10981 tttcaaattg cccgactttg gaaaaaaata cctgaaggaa ccggccctac cccagcttcc
    11041 ctaccccgcc ctggaagaat ccgaagacct tttcagcgag atccggaaac gggaccatat
    11101 gatccatgtt ccttaccaca aatacgagtc ggtgatccgg ttctttgagg aagcatccaa
    11161 ggacagcgcg gtcacccata tcaaagtggt gcaataccgc gtggccaggg aatccaggat
    11221 catggaggcg ctcatcgatg ctgcgcggga agggaaaaaa gtctccgtct tcatcgaggt
    11281 caaggcgcgc tttgatgaag aagccaacct gagatggggc gaaaagctgg aaaaagccgg
    11341 agtccttgtc aaatacagtt tccctgagct aaaggtccat agcaagattg cctggatccg
    11401 gcgtatggaa aataaaaaag ccaaacaata tgcctacctg agtacgggca atttcaatga
    11461 agataccgcg ctggtttatt ccgatctggg tttgtttacc gcggatggcc ggctcaccca
    11521 tgaggtgaaa aagatatttg attacctcga gtccggccag atacccaccg ccccgttcaa
    11581 gcacctgttg gtcggtaaaa acaatctgcg ggaagaattg tccaacctgc ttgattttga
    11641 aatctcccag gccaaagccg ggaaaccagc cgagatcatc atcaaactca acagcctgga
    11701 ggacaaggac atcatccggc atctctatca atgcagccgg gccggggtga aagtaaaact
    11761 gatcatacgc ggcatttgtt gcatccagcc ctctaagaaa aaactcagtg aaaacataga
    11821 agcaatcagc attgtggacc ggtacctgga acatgcccgg atctttttat ttcaccatgg
    11881 aggcagggaa aaaatctatt gctcctcggc cgactggatg gagagaaacc tcagtttccg
    11941 cattgagacc gcattccccg tctacgatcc taaaatccgg gagcagatca aacatctgct
    12001 ttatctgcaa tggaaagaca atgtaaaggc ccgcgacctc gcgcaccaaa acaccaatcc
    12061 ttaccggcgc aacggttcct ccatttccat ccgatcccaa ctggaaacct acagctattt
    12121 taagaacagc gccaaatgat attggcgccg aattcactaa tttcggcctg tcatgattac
    12181 agccctatcc ccgattgacg gcaggtacca ttctttgtgc gctcccctgg gggaatattt
    12241 ttcagaatac gccctcatca aaaacagggt atatattgaa atcgaatatt tccgtgcttt
    12301 gaccggactt ggattgcctc ccttggcagg agctgaaaaa cacctggact tgctttctga
    12361 cctcttcgcc cgcttcaatg agaccgaagc actccggatc aaggagattg aaaaggtgac
    12421 gaatcacgac gtcaaggccg tcgaatatta tctccgggaa aaaatcaaaa acagcccgct
    12481 tgagccttat tccgaattca ttcatttcgg ccttacctcc caggacatca acaatacggc
    12541 ccaacccttc gccctccacc ggagcaacca ggagttttta ttccccctcg ctgaggaagt
    12601 gatccgggcc atggatggaa aagcacacca gtggaaggac caggccatgc tggcccatac
    12661 ccatggccag ccggctagtc cgactaccct gggcaaagag atcaaagtat ttgtggagag
    12721 gctcaggggg cagtttgcct tgctcaggga tatcccggta acggccaaat ttggcggcgc
    12781 cacaggtaat tttaacgccc accaggtcag ttttcccggg atcgactggc acgggtttgc
    12841 agatcatttt gtgtcaggtc ttggcctgga ccggatccga tggaccaccc agatcgagcc
    12901 ttatgattac ctcgccgcat actgccatgc ctggtgccgg atcaatacca tcctgctgga
    12961 tttctgcagg gacctctgga tgtatatctc catgaactat ctcagccaga aagtgatccg
    13021 ggatgaagtg ggttcttccg ccatgccgca taaagtaaat ccgatagatt ttgagaatgc
    13081 cgagggcaac ctgggtatcg ccaatgccct tttcacccat ctggccggca aattgcccat
    13141 atcccgcctg cagcgggacc tcaccgacag cacggttctg agaaacctgg gggtgcccct
    13201 cgcccatacg accatcgcct tcaaatccat cctcaaggga ttgaccaaga tagaagctaa
    13261 caaccttgcc ctccatgatg cacttggtaa aagctgggtg gtggttgcgg aaggcattca
    13321 aaccgttttg cgccgggaag gaataaaagg gtcctacgaa ctgctcaaag aattaacccg
    13381 gggcagggat gaaatttcag aatcggactt cagggaattc atcgcccaat tgccggtgcc
    13441 cctgcacatc aaagaggaat tgttgcagat cacccctttc aactataccg gggatacgga
    13501 attttaaaaa aaattgttcc ggaatgaaca attttgtttg aatatccttt cagttgagtg
    13561 caaaaaacca ttgctgtgat cggggcgggc tttgccggaa tgtctgcggc tgccttattg
    13621 gccaagcagg gctttagcgt tactttattg gaaaaaaacc agggaccggg aggcagggca
    13681 tccctcctcg aggaagccgg ttttaagttt gataaaggcc cgagttttta ctggatgccc
    13741 gatatttttg aaaggttttt tcaacaattt aacaaatcgg catccgatta ttatgagctc
    13801 atccggctgg acccttcgta caaagtattc tggaaagacc gtgaacccat aagcatgccg
    13861 gcccggctgg aagaactcac cggcttgttt gaatccctgg agcggggcgc cggccttcag
    13921 ctgaaccgat ttctcgctga agccaaaaaa aaatacgata cgggcatggg ccggtttgtt
    13981 taccttccat ccctgagcct cactgaatat ctcagttttg acctgttcag gcaatcgtta
    14041 aaacttgatt tattttcttc cgttgccggg cacattgccc attatttcaa aaatccctac
    14101 ctccggcagt tgctggaatt tcctgtccta ttcctcggcg caaaaccttc cgccacccct
    14161 gccctctaca gcatgatgaa ttatgccgac ctagtgctgg gcagctggta tccttccgga
    14221 ggcatgtatg aagtttccaa ggcctttttt caaaccggga aggaccaggg cgtccggttt
    14281 ttattcaacc aggaagtatt gtccatccgc gcattacctg ataaaagaat gaaactggtc
    14341 acccgcggcg gttttgacca ggactttgat tatatcgttt cgggggcgga ttaccatcat
    14401 acggaaacca gcctgctgca ggaaaaggac aggtcatatc cgcagtccta ctggaattca
    14461 agaaccatgg ccccttcggc cctgctgtat tttctaggct ttgaccgcag gatagaccaa
    14521 ctggaacacc acagcctgtt ttttgacgct gattttgacc agcacgcccg tgagatctat
    14581 gaccgtcccg gatggccggc agatcccttg ttctatacca attcaagcag tcttacggat
    14641 gcccgttgcg ccccggccgg gcatcacaac cttgtggccc tgatcccggt ctccgttgaa
    14701 cttaaagata atgagaaggt ccgccagagg taccttgacc ttattctgca gcggctgact
    14761 catcacaccg ggatcgatgc ccgcaagcac ctggtttacc agaaatccta tgcccaggct
    14821 gatttcatcg acgaatacca ttcgctcaag ggcaacgcct atggcctggc caataccctg
    14881 ttgcagaccg gtccgttccg cccgaagatc agaagtaaaa aattgccgaa cttgttttac
    14941 tgtggacagc ttacggtgcc gggtccgggc ctgccccctt ccatcatcag tggagaaatt
    15001 gtagccaaat atattcatca aataaccaaa ccatgagcag ggaattattt gatcaggtca
    15061 gtcagtcctg cagcaaactg gtgacgcagg catacagcac ttcattttct atcggcatcc
    15121 ggttgttaca cccgaccgtg aggccgcata tttatgctct ctacggtttt gtccgtctgg
    15181 cagacgagat cgtggacacc tttcacgatt acgataaaaa agaactgctt gaccagtttg
    15241 tggcacagac tgaagaagcc ctggaaaaaa agatcagcct gaatcccata ttgcatcaat
    15301 ttcagcgaac cgtaaacctc tatcacattg atcgtaccct gatcgaagct tttctgcatt
    15361 ccatgaaaat ggacctggat cagaaaagcc atacgcggga atcctatgaa gaatacatcc
    15421 taggttccgc ggaagtcgta ggcctgatgt gcctgcacgt attctgcgaa ggggacaaga
    15481 ccctgtatgc ccgcctcgct ccccatgccc agcgtttagg cgccgctttt cagaaagtca
    15541 attttctgcg cgacatccag tcggaccagt ccggactcgg gaggcattat tttcctcact
    15601 ggaaggaggg gcaagccttt tctgaggaaa tcaaggagca gatcatctca gagatcaaaa
    15661 gtgacttcaa ggcaggccgg gaaggtattg tccagcttcc gggcacctgc agaacagggg
    15721 tatatgcggc ctatgtctat tatcgcgccc tcctgatccg catagccagg accccctcgt
    15781 cccggctttt tcaatccagg atcaggatca ataatgttct gaaatttttg ctcttgctct
    15841 acgccggctt gagaattaaa tggcagggga taaaatgaaa gtttatacca aaacctggca
    15901 acaagatctt ccggtcaggc tcgaaccggc ctggtccttt ttttcaaggc cggaaaacct
    15961 tcaggccatt acccccgact ttatgaactt ccggatttta acggatatcc agggcaggga
    16021 gatgtacgag ggcatgatga tccattatac cgtctccccc ctcctgggcc tttccatgag
    16081 atggactacg gagatcaccc attgcaagcc gatgaattat tttatcgatg aacaaagatc
    16141 gggtcctttc gcgctctggc atcatgagca tcatttccag gagatcccgg cgggcgtccg
    16201 gatgacggac cgcctgacct atggcctggg ctatggttgg ctgggggaat tgcttgacca
    16261 attcgtgatc gggagccgga ttgacgctat ctttgcttac cgggaagaag ttcttaaatt
    16321 aaaattcaaa tgatggccaa tgtattgatc acgatcgcca ctttttgcgc catggaattc
    16381 gtcgcctggt ctgcgcaccg ctacctgatg catggcctgt tgtggtcctg gcaccgggac
    16441 caccacgatc ccaaagcccg gcaacattcc tttttcgaga ccaacgaccg gtttttcctg
    16501 gtctttgcga tccccagctt tttgtctttc gttgcgggcc tgctcagccc ctatcctttt
    16561 tttacttata tcggcctggg catcctgatc tacggcattt gttatttcct ggtccacgac
    16621 gtattcatcc atcaaaggtt taagtggttc agaaacagca attggattta tttccgggcg
    16681 atacgcaaag cccataaggt gcaccacaag cacctgggga aggaagacgg ggaatgtttc
    16741 ggaatgcttt gggtacccct gaaatatttt aaagaagcag cggcttcgaa ggtttaaatt
    16801 tcggacagga gcttttcttt cattttcagg aattcatctt ccgtgatcag gccctggtcc
    16861 ttcatcttac ccagttccga aagtttatcg atgatgccgg ccgtggcgcc gtggctgacc
    16921 tggatcatgt gttcacccgt ttgtttttct tccttccgga tctgttcggc cacatccttt
    16981 cctttctcaa acatttcgtc gtagatcttc ttcaaccggg aaaggtcgaa atccaggaag
    17041 gtgccgccgg tctcaaaatg cttgcgaaag gcctgcccga gcgcataggt cgaagcgccg
    17101 gagagtactg ctacggccat gccgccgatg aaacttccgg ccccgggtat gaacttgact
    17161 acggtccggg cggccagcct ggccaggccg gaggaggtga tgctgctgat gatggccttg
    17221 ccttccgttt ccttgaattc aatatcgtat acccggcaaa gttgccgcac catatccagc
    17281 tgaatggcgc tcaccgcgaa aaaatcggcg acgggaacgg gtatgaatcc cgcgcccatc
    17341 gaccacacca ggtgattgcg gattacggaa tccacttcct tgtcgcgctg ggccttttct
    17401 gaaagatcat gctcggtcat atttctaatt taaggcaaat atcgagccgc caaagcctcc
    17461 gggccaattt tgttcgacta aacctccgga atcagcccct gatcccggca attcccccgg
    17521 acagccgttg aaccaaatcc gtccggaact gtttattttt atttaaattg gaaacgatgc
    17581 aaatcactgt tcaccgagcg gataccaggg gccaggtcga tttcggatgg cttcaaagca
    17641 aacatacttt cagttttggc cactattacg atcccgaacg ggtccatttt ggagcgctcc
    17701 gggtcatcaa cgacgatacc gttgccggag gaaaaggctt tggcatgcac catcacgaca
    17761 atatggagat catttccatc ccgctttccg gcgacctcga gcacaaggac agcatgggca
    17821 atcaaaccgt catccgcagc ggggacatcc aggtgatgag cgccgggacc ggcatccatc
    17881 acagcgaata caataaaaac aaagaccttc cggtcaaatt ccttcagatc tgggtcctgc
    17941 ccgacaaaag aaatgtaatc cccaggtacg atcaggtcac cctggatccc gcatcgatga
    18001 acaatcggct cacccaggtt ctttcacccg atgccgggga tgcaggcgtc tggatccatc
    18061 agcaagcctg gttccacctt ggccgttttg ataaggcttt tacccaagag tattccttga
    18121 aaaaaaaggg caacggggta tatcttttta ttctaagcgg agacctcatc ctgaatggcc
    18181 agacctacca tgaaagggat ggcttgggca ttagcggcac ggacgtggtg aaaatctcct
    18241 ctaccggccc cgctgaattc ctgctcatgg aagtcccttt gatctcctga cccggccgat
    18301 aattaaggac agatcctgat gagatcctaa ttttaagacc gaaaaataaa ccaccggatt
    18361 ggaaaagtat ttatatctgt tcctggacct cgcttcgttt tccatcccct ttttgttttc
    18421 tttcgaaaaa aaatggattc atttcatcgg ccgttggaaa gccattgccg ccgggcttgg
    18481 ggccatggcc ctgtttttcc tggtctggga ctatgcgttt accaagtcag gtgtatgggg
    18541 attcaatgac cggtacatca ccgggattaa agcatatgga ttgccgctgg aagaattcct
    18601 gttttttatt ctgattggct acgcctgcct gtttatttat gaatccctcc accacctggc
    18661 ccccaggccg gcctggggca aaaccgcaaa aaccctgttt gcccttattg caatagtcaa
    18721 tctcgtggtc gccgtcctcc acacggaccg gctctatacc ttcaccgccc tggctttgaa
    18781 tgcggtgggg ctcttcctcg tcctcctatt ttttaaagcc ttcccctgga gcaaattcct
    18841 cctgggttat gcggtcagct taataccctt ttccctggtc aacggagtac ttaccggagg
    18901 attcaccgaa gaacctgtag tctggtacaa caatactgaa aacctgggaa tccgcatggg
    18961 gaccatcccc ctggaagaca gtcagtacat gatgctgatg atgctgatca gtaccgggct
    19021 gtatgaaatg gtactcagga ggaactcctt gccaaaacag gcttaagccc ggatggtatt
    19081 ctttgcactt tggttacctg accacgaccc gcgtacccgc actgtgggaa ctgaattccg
    19141 gagcatacag gcattggatg ctggatatgc cattggaaaa atcccctttc tggctcaccc
    19201 ggagggcata ttccaggacg taggtcccgg ggctgatgtg gtcaaagaaa aaatgcgtgg
    19261 cgaggtcttt ggtactttca aaataaaaca agccaccctg gtacttgtac tcactgatca
    19321 ccccgaccgg ttcaaagccg gaggcgcgca tatcctttaa atgcacataa tccatcggcc
    19381 ggtccgcttt gagttcaata cggaccaata ccttgtcacc cggtgataat tgttcaaagg
    19441 gtttgagcac ttttttactg ccttcggttg cttccagata taaggattta ttcatttgaa
    19501 gcggaagttt gcctgtcgtt ttgattttat ccaggtcctc aaaatattgc cagtaggcgg
    19561 agccccaaac gatatggttg ttctcattat ggatctgtaa cgccgccatg gaaggtttga
    19621 cctgttcagc ggttttgatg atcttgaaat aaccgactcc cgggatcacc tggtccgggc
    19681 ccggtttgat tggttcgccg ccgatagaaa tttccggtac ccgggtctcc gggatccagt
    19741 cattgccaaa atgaaccaag gcattcactg ccgccgcggt cgatttggtg ctgggccagt
    19801 ggttcgtctg cttgttcttg agtaaccaca gtttcatctt ttcgacagaa gccctgtcgt
    19861 tagcaatccg gtcgaaggct tcaatcagca ggacctgggt ttcgatcggc aactcaaacc
    19921 aacggtaacc ggcatcaaat ttccagtaca tgccgagttc tgcatggtgc tgggcccttt
    19981 ctttgaggga ggctatgatt ttcccgggaa caatgccctt tggggtccag cgatgactgg
    20041 ccagggcgat cattgcctgg ctacccaggt tttgtttgag ccattgattt tccgcggatt
    20101 taaggaagta cccgaaagct gactgggtac ctgacggtat cgccgtttcc ttaaagaggg
    20161 aacgggcata gagatagtgc acggctatcg gagaaaggcc cacttcattt ttcccggcca
    20221 gacgcttttg ttcctcataa taccgctgaa gctcctcatc acagtaccgg atcaggcggc
    20281 tgagcatttc atccagggat gaatcaagga gtcccgctgc tttcatttgc cctaaaatct
    20341 caaggatgta ttgggaaatg taccagtcat ccctgcccgt aaaccaggga aagcctccat
    20401 tggacaattg acgctcctgc aatattctga tccctttatc tttttcggcc tgcaaccggt
    20461 ttgaatcaaa aaacagggcg atcagttttt gctgctcctg ttcagacaag gcgtctttta
    20521 cccagggcgt ttccgcaagg accgcatttt tcagttcttc attcttttgc aaagggcttt
    20581 tcagcacatc cttcgcggcc caggcctcaa agatgctgcg gatggccggg ttggaattca
    20641 tgatttttgc cccaagcagg ttggcataca tcctgttgac cacctgctct gagcagtcgt
    20701 acggatattc cataaggtaa ggaagggcct ggatggcata ccaggcggga ttggtagtca
    20761 tttccagggt ataggcgtga tgccggaggg ttccggaggc ggatgccctc aaagcatcga
    20821 aggaaaagga tttggattgg tttgcctcca cctggaccgg aagggtttcg gtcaccagca
    20881 tccggttgga aagcaccggc aggatatttt cttccccgtc cgtataggtg cccgattcgg
    20941 cccataccct gtagcccagc atgccgaggt atgctccggg gaccttaatc gtccagccaa
    21001 cggacgccga ttgggccgga gccgcttcga aagctatctc cgcgggactg agaccgaatg
    21061 cattccggag atcatttccg gtgatggggt caaagagttc aagccgtgca cggccctgga
    21121 tggcctttcc ggattgattg ctgattttag ccggcagaat taaaacatca ccctgcctta
    21181 caaaccgggg ggcgttggga aataccatca aatccttctt cgtggtaatg ctcttctcca
    21241 ggtagcctga tttcaggtcc cgggtatggc cgaagatcat cagcttccat tttgtcaaag
    21301 cctcgttcat ggtgaatcga aggctgaaat ttccctcctg atcggtcagg atatcgggat
    21361 aaaaaaatac cgtctcattc aggtttttcc ttggggtgac tatggcattg gcctggggtg
    21421 cttgcgacgg ctctgtggat cccggaggga ccggtccttc ttttttggat tctgccgcct
    21481 gagcggcctc ggggaccata gcatcgttct gcaggacttt tgtctcaatg cccatggccc
    21541 ggctcctgaa aaaagaataa tatcccaatt cgaagccaaa ccaatccagg ttgcggtatc
    21601 ggataggttt gagttcttca gcatccaaag gctccatgga aaccgtttcg gagggcaccc
    21661 cggtaaaaga aggggcggaa taaggtctgc cctggatatc gcgcggataa aacaatccgg
    21721 tattccatcc atgaggataa tattcatcca gggactgatc gtacatcgaa gccaccactt
    21781 cggccaggaa tttatctttg gcctgcccgg aaatatttag cgaccaggtt tctttttgcc
    21841 ccggaagcag ttggtccctg aaactccggg tctctatcct taggatttta tcggaccagg
    21901 gaatgtggta ctgcaattgt tccacataga accggttgtc acggaccgca tagcccctca
    21961 ggtaaaaaat ttttccccgg tcggcttccc cgatactgac cgtttccgtc gtcaggctgt
    22021 ccagggttat ccagcgggca tccgattccc ggctgtgcgt aaataatacg tgttgaggat
    22081 ttttttgaaa ctggatgtgg taattcaaga cctggcccgg ctccagcgct gttgaatcct
    22141 gaagcatctg aagggccttc caggaaggga gatttttatc tgcgaggtca tagacccaga
    22201 aataatgcac ccattctgtt tcctgctggt aacggtctct ggccagggcg accagtttgt
    22261 aggcccccgg ttcaagggtc tgattcaaac gggatgcctg acccgtctga aaggaggagg
    22321 actggatccg tttggacgat ggccaggtgg tgagatcatc ttcattccaa tacaggtcag
    22381 agggaaacca tgtcctgtat tcggattcag taatgacttg ctgatccggc cgcggccaat
    22441 accgctttcg ataccgggtt gtgggctgtt gcagttggtc caaggtccag gtccctgagg
    22501 cctggacggg tatatcattg agatcggtcg ccttgatctc aaaggacccg gacttatctg
    22561 caggcaggta ggtgccgagc tcagactgca ataccaggtt tttcttcccg atgcgcatgc
    22621 tggtggtgcc ggatcttgtt tccccattaa aatccgtgat atccacctga acggaaaaaa
    22681 taaaactttg gtcctggtca gttcccatat tggcttccgg gagggcgata aaagggacct
    22741 ggaactgccc tgaaacatcg gtctgagcct ccccttttgc aatgatcacc ggattggacc
    22801 tttgaggaaa gtagatcttc caggaccacc agggcaaggg cctgatccat cgttcgcggg
    22861 taaccgtata tctgaccata gcattatcca cggcattgcc cgcaaaattc cgggcatgtc
    22921 cccgcaccaa caccggttca ttgagccggt aaagggtcgc ggcggtatca aaggtcagct
    22981 caaaacgggg acgtttatat tcttctacct ggaaatttac cgaggcctga tttaagttgg
    23041 taaccaggct cattgaacca ctcagtccac ccgccggcaa aacaaatgaa ccgtggaagg
    23101 aaccaaaatc actggataca aacacttgtt tgctgactaa ctgtccgttg gcatcctgga
    23161 ggctcacggt gaccttgcgg tttttttgga tccgtggctg gtcttccgca tcccggatca
    23221 gggccagtcc tttaaaataa agcaattgcc ccggccggta cagggaacgg tccaggaaca
    23281 acaatgcgtc ttccctttcc ttcaatccgt tctgatcata ggaaaaatga ggaaaaccat
    23341 cctggagcca aaggctgtct ttccctttga tcaaagccac ggaatagttt ttgttggttg
    23401 gcagcgccgg gctgacggct cccgatttat ccgacaccaa tctttgtact tctttccatt
    23461 cctgggtgcg ccgattgtcg ttataagact gctcataaaa aacagcctgg accccctcct
    23521 gtggtgttcc gtgggtccgg tccacgataa agagggtttt ggtaaaatcc ctgcccaccg
    23581 taagcctggc ctgatacccc gcttcatgca gatatgcaag gttggataca tgggtgtaca
    23641 tcagagcgat gacgcccttt cctatctgaa agtcggcatg gttgctgagc agaataccat
    23701 acattcccgg tggcagggca tcgattttta attcggtccg gtgttcctgg aggtcttcta
    23761 cggaaggcaa ggcctgctga aagttttgaa gcggggacgc ctgcagcagc agttgaatat
    23821 tctccggccg ccggtcttct tggagctttt tcctaagggt gtccggcact tttattacac
    23881 ggccatgaat ccttggcgta ttgcggtact ggacaaacaa ccggaagggc tggccgggga
    23941 cattcacttg ttccacctgg ccctggacct gtgcttggac caaagctttg atttgatttc
    24001 ccgcctgaat ggcttccctg ctgcccggat attttgcaat gacttgttcc aaaatggacc
    24061 ttgcttcggt cagccagcgg cggtacacac tgtcttcacc aaaaggttcg taccggttgc
    24121 cccattgaat gtatagattg gcttttaaaa ggtagaaaat tccctgatgc tgaggataaa
    24181 gagccatcca ctggtccagg ttggtttcgt acaaggcgtc tttcccttcc cctgaaaagt
    24241 gttcatatac atattggatc cgcttctgca cgacatccag gtatgccgca ggatttgaat
    24301 ccttttggtg gaattcctcc agggtctgga acaaatggat gacctgaacg taaaacacgg
    24361 ggtcttcctg ccggtattgc ggattaatct ctaaaaactg ggaggttgga ccgaatagcc
    24421 ggggatcatc aaagcggtac tcgtcgccgg ccaggtctaa atcagacatc ccctgcataa
    24481 aataatcaag cgcccggtgg gccagaatat catacaagaa cggcctgagt gaatcactct
    24541 cttccccttc ggtcagcaag cccaggtaat ttgaaaccgg tacgtttttg aggccggggt
    24601 gctccatagc ctgcagatgg tgttttctta ttttatttaa aaagtcaacg gcagaccagg
    24661 tctccggaga accggcgtcc agggtatctg aaagaaccgg ggtacggtcc cggatgaccc
    24721 agatattgga cctgaaataa ccctggtaga gttcggcgag cagggaatgt ttgatggatt
    24781 gaccggtccg gcttcccaaa tccaaatcag attctatcag atggatcgct gccaggagcc
    24841 cggattcctc gagtccggcg ctgtattttg caagatagat cagggatttt atggcattga
    24901 tctcctcgtt ttccttcttc gccttttcat aaaccagcag ggtttttgtc ctggcggatt
    24961 ccggcaggcc ttctttttcc agggaatcaa tttgtctcca taaagttttg taatcggatc
    25021 gcagggccat ggtgtcctgt ttatttttag aactggcgca ttggagatgg agcagaaata
    25081 aaacagcagt aacccatccg gcaaattgta gtctcatatg gaggcagatt gtaattgaag
    25141 agatgcatgt tcaaaggcta aagttagcct ttaaatgggg attaactcgt gattaacaag
    25201 ccatcacata gcggaaacag ggagcaggaa atgctcctcg cattaattac cgggaggagg
    25261 gcctgaaggc ctttcaggca tgaccaggct gtccgccggg tatttcatct tcatggcatc
    25321 catcacagac tgggcccagg ctatgatttt tttccgttgg tccgtactca gtttggcgtc
    25381 tccatgggta agggtgtatg atttcaacgg catttctcct tcatccacca tctcgaccac
    25441 ttcctccatt ttatgatact gcaaggcggt cctcctgccg gccagttcgg aaaaattaag
    25501 atgtcttttc ccatctttga catgatcggc cagccaccag gcaatgggtt gaaagttgtt
    25561 gtaccaggga taccgggtat tgttgctgtg acaatcatcg caagccagag ccaggatctg
    25621 ttttacatca tcaggcatcg gataaagcgt gttgatgtgt ttggattgat cgttcccctg
    25681 gtttttagcc gggcggataa actggatcac cactaaaaca aacaataaac cgattaggat
    25741 tttacgtatc atcttcgcct gaatttagta actaagataa tcattctttg aaccgggaag
    25801 gactgaaccg gttgattttt tccctgccat tcaaaagatc cgcaaggtgg cgggtcacat
    25861 aagggcccag ggaagaacct ttggtgccaa atccattgac tgcccacaac cctggcaatt
    25921 cggcatgttc ggcgagtatt ggtttacggt ccggaacggt tggtctcagg cctgccatat
    25981 gggatctcac ccgataaggt tgactgagca aattttccaa tctgcggtac aggtaattcc
    26041 aaccgtaaga atccggaccc gggtagtcaa aatcccattc atacgtggct ccggcccaaa
    26101 tcacctcttt ccatctggtg agtaggaggc cggattttac caggtagtcg atgttttgaa
    26161 acccggcggg atccaaagtc aaaacctggc ctttgttggg aatcaccgga aggtgactaa
    26221 accatggatt gcccctgcac cagacgcctt cgcaaaagat aacctggcct gccactacat
    26281 ctttatatct ccaggaatcc ccttcacggc tcaaggccgg atactccatg tgctccggta
    26341 aatacctttg ctttgctttc cagcgattcg ccgaatggcc gaggagcccc tggatatcca
    26401 attgataagc gttgatggca tatcccctgc gggaattctg catatcccag ccattgaccg
    26461 taaggggtcg caacaaatca tcataagtat accgccggga ctggctcagc aaatcatttt
    26521 cctcttttac tgaagagaca tacaaatgca tatccagttt ccggaacagg ggcaccttca
    26581 gattccgttc aatttcttca taggtttcat ggcattgggc aatgagttga tcataaagcc
    26641 aggctttaac gaacttccgg ccactgaccg ggtgaatgat gccggcagcc agagaggagg
    26701 cggcatgggg cttttcaagg cccatgataa ccaccgaggc acccatcctt tccagggcgt
    26761 gaccggtgag ggtgccggcc aggccttgtc caacaataag aaaatcagtt ttcaccggag
    26821 gcttcctttt tccctctgcg cgggtggagg accagctcgt acgatagatc gatcagtttt
    26881 ttcagcaaat cgcttttcag gaatccgtcc aggtaaatgg tgttccaatg cctcttgttc
    26941 atatggtacc cggcaatgat ctcaggatgg ccttccctta attcaagcgc ataatccggt
    27001 tcgcacttca ggtttaccca tggcctttcg gaactcaggg caaggatcgc aaaaatcttt
    27061 ttgccggctt taaacaccag ggcatcgggc ccgaaaggca ggctttcggt caccccgggt
    27121 ttgtccaggc aatatgccct gattccatcc agtcccatca atagatagcc atgtcaaaat
    27181 atttccgcag gcttttggtt accgggtggt ttttcccata aatatgaaac aaggcgatcc
    27241 cgcttttccg ggcggtttca tccatgtagg atttatcggt ttgcagggat tcaatgattt
    27301 tttccattcc tttttcgggc tggctgtgat cgatcaggtc gcgcgcttcg gtcaataatt
    27361 tgcccggctt gctgtcgtca aactgatgaa caaagaaagt agccagcgtt cgcacatcct
    27421 gcacccggtc aaacaaggga tttccttctt tgatatgctg ggtgagggcc tctgcttcag
    27481 ccgggtcgga gaagaccagg tgagaagcca ggtatacttt ggccttgtcc gaatccgggt
    27541 tttggcccag gtattcatcc aggtacatta caaattcggg gtccgggacc tgggtgattt
    27601 tatcaaggat ttcatcgggg tccggcaatt tcatcccggc ttcctcctcc ccttccgtcc
    27661 cgccttcctg tccggcaagg gcatccatat ccggtaaatg tttggccagc caggcctgga
    27721 cctgtggttt cggcagtgca ccaacaaact ccccgatgat cttgccctgg tagatcattt
    27781 ttactgccgg aatgctcatg atattcaaag cctgggcgat atcctggtgc tgatctgtat
    27841 tcaatttggc cagtacccag gtgccgttgg cttcctgggc caggctttcg attacggggc
    27901 ccagtgcccg gcaagggccg caccagggtg cccagaaatc caccaccacc ggcacttcat
    27961 aactcttatc gaccacttcc tttctgaaat cttgtacttc catttggttt attttttact
    28021 ttaaaattaa tttctgttcg ccaacaggta gaggacggcc atgcggatgg ccaccccgtt
    28081 ttccacctgt tgaaggatga tggactgctt cgaatccgcc gcatcggaag ataattccac
    28141 tccacggttt atgggtcccg ggtgcatcag gagtatgggt ttgtctatac ggtccagcat
    28201 ggcggcattg attccataat actgagcata ttctctgagg gagggcaggt atggaatctc
    28261 ctgtctttcc agttggatcc ggagcacgtt ggcggcatca caccatctca gggtggattc
    28321 cacatcgtgg ctgacctcca cgccaagcga agcaatatgt tttgggataa gggtaggcgg
    28381 gccgcagacc cggaccctgg cgcccagttt gttcaggcaa aaaatattac tcagcgctac
    28441 cctggaatga agaatgtccc cgatgatggc gatctttttg ccactcaaag ggcccagttt
    28501 ttcaatcagg ctataggcat cgagaagagc ctgggtggga tgttcatggg tgccgtcccc
    28561 ggcatttatg atattcgcgc tgaccctgtt ggcaaggtat accggcacgc cggcagccgg
    28621 atgcctgatg acgaccatat ccactttcat ggagagaata ttgttgaccg tatccaggag
    28681 ggtttcgcct ttgctgacgg aagaggagga agccgaaaaa ttgatcacgt ccgccgagag
    28741 cctcttttca gccagttcaa aagaaatccg ggtccgggtg gaattctcga aaaagatatt
    28801 ggcaatggtc acgtccctca gggtaggaac ccgcttgatg ggccggttga tgacttcctt
    28861 gaactcgtcg gcggtgtgga atatcaactc gatatctttt gtggtcacat acttgatccc
    28921 gatgaggtgt tttgtactga attgcgcctg ttcacccatg gatcagtcct cctgtttttt
    28981 cttgtacaat ctgacttcat ttttatccgg atcggggtcc caatccacca ctacatgcgc
    29041 ttcgtccacc gcatcgacgg tgtatccaat ataatcggcg gaaacgggga cctcccggtt
    29101 gaaccggcgg tcaatcagca ccagcaattc cacctccttg ggacggccga aatggttcag
    29161 ggctgtgagc gccgcctgga tggtgcgccc ggtgtacagg acatcgtcga tgagcaccac
    29221 ccgttggtct tccaccacaa aatccatttc ggtctcattg gctttcagcg ctttacgcaa
    29281 tctgaaatca tccctgtaaa aggtgatgtc gagttttcca tacctggcct tatgtccggg
    29341 attgatctcg gtgatcaggg tataaatccg gtctgacaat tctactcccc tgggttggat
    29401 tccaacgagg caggtatttt caaagtgatt gtaattttca atcagttgat gggccaggcg
    29461 ctgcagagta aggttgaacc ggggagactt gaggatgatg cggccacgtt ccatgtcgcg
    29521 gtaaaattac ttgaatcccg acaatagtcg aaatcattgt tgcatgagcg cccttgcata
    29581 ccgcctgccc agttcacgca gcgaagcgga attaaaatga atactgtctc ctttgctttc
    29641 aaggccttca gaagaaacca ggaataagtg aggggactcc ctggtttttt tcaaaaggag
    29701 ctcattgacc ggaatatgaa aaccgtggaa ttgattccag gataaaaatc gggccagttc
    29761 gcccatgagg ataggcagat ccgcatgacc gcaggtctgc ctcattttca ggaagaggct
    29821 gtccaaaatc atggaatagg atgaaagggc agctgcattg gcattggatt caccctggtg
    29881 ccagaggatg ccttttattt caccatcctt tttggcaata tccaccttcc gttcaaaatt
    29941 ggtatataac cgcactcccc ggtacaggga gtcatataac caatgttccg ccgaactgcc
    30001 cccaacggca cagggaataa gcccaatccg gatgctggcc tggttaagca ggagcatttc
    30061 ctttgcaaaa gacaggccgc agtccagccc ggtcctggcc ggttcatagt agtggagcgg
    30121 ctcccgagcc agggcccatt ggtttgtcga atccaggtaa agcactcccg gatggctcac
    30181 cgtgtcctcg gcttcgatca agcccctgcc ggccatattc gattgaccgg ccaggatata
    30241 gatccacatc ggtttttcat gctttccctc cgtggttacc ttacaggaga agcctgctgc
    30301 cagagacagg atcaataaaa cggtagtaaa tattcctttc atcacgaatt aaaaatagag
    30361 attcagcacc aggaaaaaca acgaaggaca gagcaggcaa cccataccgg tataaaaagt
    30421 cgcggtcagg gctccatagg gcaccagcct ggggtcggtg gccgccaggc ctgcagctac
    30481 gcccgaggtg gtaccaagca ttcccccgta gatcatggct gtctgaggat tgttcaatcc
    30541 gatccattta gccaccaagg gggtaccgat ggtcaccgca atggacttga ccaccccggc
    30601 ggcgatgctg atcgcgatca cttcagaact cgcccccaat gcagtgccgg tcaccggtcc
    30661 aacgataaag gtgcaagccc cggcgccgat cgtagccagg cttaccgcat cccggtatcc
    30721 aaacaataag gcacaggccg cgccggagaa aaaagtaagg atgaggccca ggaagatcga
    30781 tacaaaaccc aaccagcctg ctttccggat ttcatccagg cttgcattca tcgccgtggc
    30841 cactacggta aaatcacgaa gcatggtacc ccccaaaagc gcaagttcgg acattatggg
    30901 gatatcggct gcaccattgg gccctcccgc tataaaagca gccataaggg ccataaaaat
    30961 agccaaggcc acggtgggaa tcttgttcct gaaaaccttc tttgaaatga gttcagcaat
    31021 caaaatcagg atcccgacgg agagaaaggc tgtgatcagg ccgtttttta tcaggatttg
    31081 cgggatcacc gggaggtatt gaatcgtgaa gccaaaaagg ggataaggat aaaacaaaat
    31141 gcagtgggta gtatacctgc caggacagcc aggtatccac tcgaaaaggc cattcttgca
    31201 ttttgaatgg cagacatggc cacgacaaca ggtatataca tcctgctcca gaaagagatc
    31261 ccggacggaa cctcccggac caaccatttt ttggtaagcg gccagctttg aaagaggatc
    31321 aggaggatca ttccaaaccc taccccaccc acattggatt ttactttcat caggctggcc
    31381 aggccttctc ccatccacag gccggccagg taacaaaagg ccagcaaagc aacaccgtaa
    31441 atggtcatgc ggtaaatata gtttttactt cctgtttcac caccttgccc atcgcattcc
    31501 gaggcaattc tgcaacgaat cggtacaacc ggggcatttt atatcccggc aatttcttca
    31561 acaggctttc tttgatggca gggatatcct cttcaccaag atgcggggat tgaatggcgg
    31621 ctgcaataag ctctccccat tcttcatcgg ctatccccac caccgcggta tcttttacgc
    31681 cctcgatagc cctcaaggct tcttcgattt ccagagcgga aattttataa cctccggact
    31741 taataatatc caccgaattt cttcccaaaa tccggaaata cccatgttca acaagtgcaa
    31801 tatctcctgt cctgaaccat ccgtctgagg taaaggattc cctggaggct gcgggttttt
    31861 gccaatattc tttaaaaacg ttgggcccct tgatttgaat ctctcctggc tggccttccc
    31921 ggaccggctt gccctcttca tccatcaggc gcactcccac tccgggcaag ggcatgccca
    31981 catagccggc tttccgggcc ccgtgaaggg ggttgctgat cgccataccg atttccgtca
    32041 tgccataacg ttccagcaag gtatgtcccg taatgacttt ccattgatcc agcacggaaa
    32101 ccggcaaagc cgcggaaccc gataccatca gcctgaaacg gccaaaccga tccctgagtt
    32161 tttgtttagc ctccggatcc aaacggttga aatattggat cagtttgtaa tatatggtag
    32221 gcacagccat aaacaaggtc aggttttctt ccaggatcaa atgaaaaacc ccggatggat
    32281 caaattcggg cataaaatgg acgcaagccc cgctccataa ggcgcatccc actacattaa
    32341 taatcccgtg aacatgatgc aagggaagaa aactgatgat ctggtcctcg ctgctccatt
    32401 cccaggcttc aaccagggtg gtaatctggg aacgaatgtt ggcatgggtg gatacgactc
    32461 ccttgggttt atccgttgtt ccgctggtat aaaggatcat cgcgtccctc tggccttcaa
    32521 tccgcggaaa gaacggcttc agatcctgat cttccaatac cttttccgta tccgtcaggt
    32581 tgagctgcat accggatgca aggggctgaa gcgactttac atattccgga gaacaaatga
    32641 ccacctccgc gccactgtcc cggagataat aacggacggc ctcttcggga tggttcaggc
    32701 ttaaaggtac ggcaacgccg ccggcctgcc atatccccca ttgcacagcg acataagcaa
    32761 taccaggatt gaccatatag gcaatgcggg cgcttccaag gtcgtctttc cctcttaaaa
    32821 gaaaggtggc gaccttgccc gctctaagaa tcagttcatc ataggtaaac gctttgatcc
    32881 cttcccggat ggctaccctg cctttgaatt gaatggcctg ttgaattata aaagtcatga
    32941 ggattcaaat gaagccagtt tcctgtttag tgcagggagg ccggggtgat cactctttcc
    33001 gatttagact tcatatggta aagggtgatc cgcgaaacac cgggacctga aacctgtttg
    33061 gaagaagact gtccattgac tgcggcgccg gcaccccact cgattttcct cctggctcct
    33121 gaggaaaact ccatcatacc ggaatattct ccgggttgga aggtaaaggt catgcccggc
    33181 gccttgaaag ccgcaacagc cagggagtcc aggtttcgca tggcgatgag ccttccccct
    33241 gctcccatgc tcaaccggac cagggctttg ctgtccccgg ggatataaat gccgctttcc
    33301 tgaaagggca gtgatttaaa tcctccatgg gtatcgttga tcagtaccag gccctgcatg
    33361 gcatccgccc gtccctgggt aagttcgatc ccatagtcat tgccggtgag tacaaggtcg
    33421 aggaattcat ctccattgat atcctgggcc accataccat acaacggggc aaactgcgct
    33481 tccacaggaa gggtgtcaac cctgaagtcc cctttcccat cattgaccag gaggacagaa
    33541 ttcatattgt tggctttgag gatcatagcc ccttccagct ccttgggtgt aaaaacctgg
    33601 tttacggtgg ccaggccaaa agaggtatac gtctggaatt tcctgcggat cagaatcaac
    33661 tgtttgatca tatcgtccct tccgtgataa aaatactccc gtttaacccc cagggtatcc
    33721 cgccaataac aggaaatgaa aggatcgagg ctgccattgg aatcaaaatc cttggcatag
    33781 atcgtcatgg gttcattggc agagcaagtg aaataaatat tttttccgta attgcctgcc
    33841 acatagtcca tatctccgtc gttgtcaaaa tccccggcca gcaggccggt ccaccaaccc
    33901 atgtgccggt ccaacgccgg tgcactcagc cgggaaagac ttttaccgtc attcctgtat
    33961 atggtcaccg gcccccattc cgtggccaac aaaagatccg gccgtccgtc attgtcatag
    34021 tcagaccaga gggcatcgtt gactatgcct gcctggccaa gtcctgcatt ccaggcatcg
    34081 ggggcaaggc taaaccgggc ttttccgccg gccgcttcgt tgagcagtat aaaactttta
    34141 tccgccttcg gataatatcc cggcaatacc cttccaccga taaaaaggtc caggtctccg
    34201 tcctggtcaa tatcggctgc ccttgccacc gaagcgcagg tcctggccgt tttagggatc
    34261 gccagggtat ccggtgaaaa attgcccttc ccgtcattga tcaataacag gacctggaat
    34321 acggatgcat cagacatcag gttttcatag gagcccctgg caatcacaag gtccaggtcg
    34381 gtatccccgt ccgcatcgaa caaaaccaaa gaggcatcct ctgcatccgc atccgattcc
    34441 tttttgcttt gaagcgcctt tttgttgaat ttcccggatc cgtcctgtaa atacaccatc
    34501 tcctggtgat tggcgctgcc tcccaggata agatcatcga gtccatcgcc gttgagatct
    34561 ccgacggcaa gaggagttcc gaattgggac attttatggg gcaccgtttt ctggatatta
    34621 aaatcaataa aatccttttc ctcgtgtttg taatccagtc caagagaggc cggtttttcc
    34681 atactgaaaa gcccttccgg ggaaggagcc aactggcctg aaaagggctg cccctcaaaa
    34741 ccataggtaa ggaattggtc agcctggata ttgccggcga tttgccggct tccatccggc
    34801 cattcgatag agatcgagtc aacgagggtg gcttctccca ggccaaaata taaggttcgg
    34861 gatggttgtg aaagataacc cctgccgctt aagaccagcc tgctttgcat gcccttcccg
    34921 gtatatacgt tcaccttcgc accgatcgca tcaggatttg aggccggacc ctttaaccgg
    34981 acctgaagaa aatgggtggt attggttttt cggtccagtt cttccgtctg gtttttaaaa
    35041 agaaagggtg gatcatcgat gttatggacc accagatcca ggtccccgtc cagatcgagg
    35101 tcggcatagg cagccccgtt tgaaaaggac ctgatggaca agccgctggc tccagtgatg
    35161 tcttcaaaac ggagatcccc tttattcctg aataaaaaat tcgggatttt tacgatcggg
    35221 atcaggcttt gcagatccat atcgctgacc aggttactgg cctccgcccg gtattcccca
    35281 aaatcgtgat cggtgacgtc tttcggaaaa ccattggtga tgaacaggtc ctcataccca
    35341 tcccggtcaa agtcggcgaa caggggagcc caactccact ccgtttcatg caccccgctc
    35401 aggaagccaa tttcgctgaa agaaggcaat cccgtttccg gaaccaggcc attgttgagt
    35461 tgcaggacat tccgggtaaa ctggtattga tagccatact cctggatata caggtaatac
    35521 gaataattgg tgggacccag cagggttttt ttccggagat tggtattcgg cagcatttca
    35581 gcggtaacca cttccatccg gccgtcgttg ttgatatccc ccaggtcgct gcccatggcg
    35641 gaagaggcca ggtgcctgaa tatatccgcc aggcgattgg agaaagtccc gtcccggtta
    35701 ttgatataaa tcaggtcgtc actgaggtaa tcgtttgcca cataaatatc cggccatcca
    35761 tcctcattaa aatcataaat aagactgctg tggctgtatc catgtaaatt caggcccgct
    35821 tcatgagata tttcggtgaa gcgaggatga cccgaaaccg gatccgggtc atttcgatag
    35881 agtttgtcgc aattgagggc acaatcgttg tggacgtctt tcacgaaggt acccggccgg
    35941 ggcctgtcga taaaattagt ccctatgaag aggtccagat ccccatcccg gtcgtagtcc
    36001 agccattggg catgggaagc atgggaggtg tcatccaggc catattcctc cgcgctctct
    36061 ttgaataccg ggatttcgcc ctggtttccc tgattgatga acaataaatt tttccgctgg
    36121 tctttattcc ggatcagggt attgcataca taaatatcct gtttcccatc agcgttgaca
    36181 tcaagtatat ttaccccgga cgaccattgg ccctccctct tctgcacccc ggcggcttcc
    36241 gtaatgtcct taaatttaag atttccaaga ttcaggtaac accggttacc cacctggtta
    36301 cccgcaaaat agaggtcttc caggccatcc ccgttgaggt cccctatggc cacccctgcc
    36361 ccattgtaaa taaactcggt gttgaggata ttgaaggtat cgtattccgt aatctcgttg
    36421 acaaaagtga gacccgatga ttcgggccgg atgggtgtaa aaaggtcggt tcgggtcctg
    36481 caggccagga acatcaggaa gcctgccagg ccaaatggta tccatttcat aatcgttaca
    36541 aatctacgaa tccccaggta tgggtaattt aaagaaagcc taaaaatacc agctaaagca
    36601 tcgatctcct cagaatataa tcggaaagaa gctcaaaaaa aaaggccacc ggttttaagc
    36661 cgatggccct tttacttgta ttaatgacta attaaaatcc tactaatcct actaatccta
    36721 ctaatcctac taatcctact aatcctatca atagcttgga ttctgagtca ggacatcgct
    36781 gccctggata tcaatttggg tttgcggaat aggaagaaat tcccttccat ccctgaaggt
    36841 agcgccccca aacatggtaa ccaaaagcac cgcctcatta gccaggtatt tgttcaattc
    36901 agtcttagct ataccccagc gaaccaggtc aaagaaacga tggccttcgc cggataattc
    36961 gagtttacgc tcaaagcgga tggcgttcaa agccgccgcc ttgtccgtcc agggggtatt
    37021 gtacaggccg atcacataat tagccgcatt ggatccgtcc ggttttttaa cccaggaagc
    37081 cggattggct gcacgggcgc gaaccagatt tacatattcg cgggcctttt ccagacttcc
    37141 accttctgct tcggcttctg ctgccataag caatacatca gcaaaacgga tgatggtgta
    37201 gttcatggtg gcatatcccc gggtccatcc ggaaccatcg gtcagcgtat tttcctgtgt
    37261 tttgtaataa acatacttct tattggaata tggtcccgca taagcctggg agcgaatcca
    37321 gtttttaccc gggtgggcaa tccaatccaa atacgggatg ctcctccggc caaccgaatg
    37381 atccaacctg ggatccagat ttccggcatc gggagtgaac ggggcagaag actcgatgtt
    37441 ataatcgtgt ttcaaagcat tggctgcact gttgtaagaa ccatccaaaa gaggcaggcc
    37501 attggcatct gtacggaagg aattggccaa ttcaaaactc ggttggaaaa atccgcagca
    37561 gtttccaggg ccgtcagtac cggtattgta gggatagttc agaacgtcaa aatggttaga
    37621 attattgaca cttcctgaat tgttggaact ttgtacagca aataccgatt cttcatggtt
    37681 atcgttggct gcattgaaga tttgggcata attggccacc aaaccatatt ttttaccatt
    37741 ggtggttttg ccgttggcaa tcaccgcgtc aaaaatggct ttggcatcat tccatttctt
    37801 ctggaacaaa taagcttttc ccaggtaaga gcccgcggcc catttattgg cgcggccggc
    37861 ctgagaatga gtttcaggca ggttgtcgta ggcaaatttg aaatcggctt cgatcttatc
    37921 ccagaaattg gggctgttgg gtacggcttc tattccggtt ccataatcca ccgtctcatc
    37981 gatataagga acactgttga atattttctt gagttcaaaa taataatggc ctcttaaaaa
    38041 gcgggtttca ccctgaatgc gtttcaaatc ggccgcagag atatcggttg cctgggcggc
    38101 cagcctgaga acggcgttgc atcgtgagat gccttcatat ttggccaacc aggtcccgtt
    38161 gacatcccct gaagtaggtt ccgtttcata acgttgaata gggttgatgg aactgaaatc
    38221 accgggatcg gttcctttat tggcttcacc cccgagtata ctgccgaaaa cccaattgtt
    38281 gggcgcggcc acctgtgtat atcctttgct gttcagcatg gaataagctc cgatcaaggc
    38341 tccttctacc ccagccttag tggccaattg ggcattcccc agggagcctg taggcggaac
    38401 ctccagaaaa ttatccttgc aggcgaaagt aaacaggagc atcgcggtca gcgacaggct
    38461 tactttcctc caattaaaat ctgtcttcat tatataattg tttaaaagtc ttttagaata
    38521 ttttaaaatc caacgttaat tcccagggtc ataccacggg ttaccggata gttgcctaca
    38581 tctaccccga aattggtatc cacatcgcca ccaacacccg gatccaatcc ctggtatttg
    38641 gtgatggtga aaatattggt gccggccagg gtcaaattca ctttggaaag tcccaagggt
    38701 ttgatcaggc tggtggggag ggcatatccg atggacaggt attgcattct cagataagaa
    38761 ccgtcttcca cataccagga gttttctccg gcattggtgc tgacgttgga ggcgctttcc
    38821 caaatgggtg ctttggcttt gtcccctaat gctggggtcc aggattcttt agccctttct
    38881 cccttaccag agccttcaaa agaaccaaag aagtcggtaa accatttgga gaagttaaag
    38941 atcttattgc cgagggaggt gtacaaataa gtaccaatag tcaggttttt ccaggaggca
    39001 ttgaatacca gacctccggt gtatttagga accggatttc cgataaaggt acggtcagtg
    39061 ggatcaattt tgccatcacc gttgatgtct tcatatttga acctgccgac ccccttgcct
    39121 acctgagcag gtgaattggc cacatcggcg gcatctttga aataacccag tactttataa
    39181 ccaaagaaag aggataaggg ttgtcctacg gcattccgga tcgggctcac ccctctgtat
    39241 gcattgcccc cgaagaaggt cacattggga gcaaggtagg tgatttcatt cttaaggaaa
    39301 gaactggtcc agctcacatc gtaattccag ttggagctga ttttaccacg gttgatcacc
    39361 aagaggtcaa atcccgaatt gttcattttg gcgatattga ccgccggtgc agctgcaaaa
    39421 cttcccacta ccccagggag cggtacggta tacaggagat catccgtttt tttcttccac
    39481 agatccccga caatttccca tttgccatcg aacaaggtag cgtcaaatcc aacgttgatc
    39541 gtggttgaag tttcccattt ggcgtccgga ttcccgatac gggaacggta ataaccttca
    39601 ttcaccccgc tgttttgtcc ctcaatggga taaaacgagt tgccccggtt tgcagcatat
    39661 agtgaatatt ggttagccgg atccacgttg ttggaattac ccatctggcc ccatccacca
    39721 cggattttca ggtcagtcag gacttcattg gcgcggagga aatcttcacc cgtcaccctc
    39781 caggcggctg cgaatgcagg aaagacccca tacctgttgt taatcccgaa acgggaggaa
    39841 ccatccctcc ttaccgttgc ggagatgtaa tatttatcat ttagattata atccactttc
    39901 ccaaaaaggg attcaaaatt aactccactg aacaaaccgc tgtttacggt cgggctctgg
    39961 actacgttta gattgacata gtccaggtcg gtggaaaaag gattcagacc ggaaccattg
    40021 atgaaacggc ctttcccggt attcagaaac tcagcaccgg ccagaacgct gaagccgtga
    40081 ttgccgactt taaaattata agttgcggta ttggtattca cccatccaaa actattctgg
    40141 cttccttcac tgaaagagtt gctggcttcg ggttcagaat cacccaaata cctgtagttg
    40201 taattcttga agaaattgga accaaaatct cctccaacgc tgctgcgaag aaccagtcct
    40261 ttgatgacat ccagttccag gtaaagatta cccaagccgt tcatattgaa tgtccggtcg
    40321 ttctgggtat tcaagactct tgacctgacc gggttcctgg ggttgttgaa gcctgcggcc
    40381 cgggtactgg catattggcc gagttcgtca aatacgggaa tgaccgaagg catccggtaa
    40441 gcggataaaa cggtagattc gtcatcggcg atacctatac ctcccgaacc tccttcctgt
    40501 ccaactactg atctgtaggt aaactggacg ttttgaccaa aacgcaaacg gttggtgagg
    40561 ttgtattcca tattggcccg gagagaatac cttttgaatt tgttttcaag aaggatcccg
    40621 tcctggtttt gggcgcccaa ggaaacataa aaacgactgt tttccccgcc cccgctgaaa
    40681 ccgagggaat ggcgcatgag gccggcagtc ctggtgattt catcatacca gtcggtccca
    40741 gcaaggtttg gtttgatcag gaataccgtc ccgggattgg ctgcggctgc cgctttgatg
    40801 gcttcgacat ccacgctacc ccgaactccg ttcgcaccat tggcgtggag gtaatccgga
    40861 agggtagcct gggcctgagt gccatattga ggatggttgt acttaacttc cgtacctgta
    40921 gcggcggcgt tattacggta agcaatatgg gtccagtccg cttgctcctg tggagatagc
    40981 attttgggtg cccccgctac gttggggtcg gtgaaaccga ataaaccatc gtaggtaata
    41041 ttcacttttc tggagttttt ggagcctttt ttggtggtat aaacgatcac cccgttggct
    41101 gcgcgggctc cgtagatgga agcggaagcg gcatctttca aaacggtagt cgactcaatg
    41161 tcatccggat tgaggaagtc ggtggaacct accggttggc cgtccacaat ataaagtggc
    41221 tggttacccc ctaaggctcc aaatcctctg accctgatga tattggtggt tccgggctgg
    41281 ccattagtca ccaccgtgac cccggatacc cttccctgaa gctgttgctc cacgttaccc
    41341 gaaggaaccg ctttcaattc tgcggcttta accagggaca ctgcaccgga aaagtctttt
    41401 ttcttggtcg tcgtgtaccc ggtaaccacc acctcatcga tcaatacccc ttcatccagg
    41461 gcaatattga ggaccggcgt ggcccccacc gtcacctcaa tggcggtgaa accggtgtag
    41521 gaaacctgaa atacatcccc ttcttttgca gcaatactgt aggatccgtc gacatccgtg
    41581 acggtacctg tagtagtccc tttgatcagg atcgaagccc cgatcaaggg ttcacctgta
    41641 gccctgtccg tgactacccc ggtaacctgg gcgctcacag aaacggtgag aagcagcagt
    41701 ccagccgaga gaaacgacct gaaagctttc ttcagttttg gagtaaaatt ttcccttttc
    41761 atacaattta ttaagaatca aaatttaaat tttcaatctg cgagttatag ctaatacatc
    41821 caaagtatcc gtatataacg tatcggacgg ccaaaaataa catttgatca ttaaaattct
    41881 aaagaaatgc taaattttta aatatgtatg atattttttt aacatttttc aatagttcaa
    41941 taaatacaat aacttgttaa tctgtcagat agatagaaaa aggaaatgtg aataaaaaaa
    42001 ggagccgctt tccaaatttt cgcctcagac ccctgttcgt tgtctgattc aatccccttt
    42061 cgatttataa ttgcaccggg atcggttgcg gagaatattt aattcgtccc cggtattatg
    42121 accgccggtg ggtcgatttt tcttctcaac attctggtat tccaaaacca agcccgaggt
    42181 tccggatccg ggaccaaatt acggtgcccc ggcccgggaa atattattaa aatttcaatt
    42241 tggcacaatt tctgaggata cccggacgtg gtgaatattg aagaaatcaa tcgcaagcac
    42301 atcatcaaga ccgattgtct gtaccgggtg ggctatggtt taaaatccag gcttttggat
    42361 tttcaaaacg ggatcattta cctggagatt gtttttggca gccgctggca gaataattat
    42421 tccgccacgg tttatgaact ggcccactgc tggaaggaca acaatcccga actcaggcat
    42481 gcccaaggct gcaaggtctt tatcattgac ggtcgcaaag accctgaaaa aatcgacctg
    42541 caccatgccg gcatccgcgt ggactatgac gcaagaaaag ggatcctgtt tgcgccgttt
    42601 accctcaatt gatcaagcag cgttcagcta tcagcattca gctatcagca ttcagcattc
    42661 agcgttcagg aatccgcata ttttcccgct ttatatccag accacccgct gacggttgta
    42721 taataagagg caaattttta ttatccacaa acgaaacttg gaagcttagc tgaattccag
    42781 gggttcgaaa ccctgtctgc tgccaggcag gcttccaggt cttgttacta aaattaatat
    42841 tactaaaatt tgatgtaccc cctgccaaga tcctgaaaga ccactacaga gcttttctta
    42901 cttttgtgcc tttatggaat ttaattcagc ggcaatagaa gcaaagtgga aacggttttg
    42961 ggatgaaaac caaacgtata aggtttccaa caccagcacc aggcccaaat attatgtact
    43021 ggacatgttc ccctatccct cgggatcagg ccttcatgtg ggccatcccc ttggatatat
    43081 cgcctcggat atttttgcac gctataaaag gatgaggggc ttcgaagtcc tgcacccgat
    43141 gggctttgat tctttcggtt tgccggcgga acaatatgcc atccagacgg gcatccaccc
    43201 ggcggtttcg accgcccgga acatcgcccg gtaccgggaa caactgggta atatcggctt
    43261 cagttatgac tggagccggc aggtgagcac cagtgacccc gcattttaca gatggacgca
    43321 atggatcttt attcagcttt ttcaccattg gtatgacctc gaacaggaaa aggcacggcc
    43381 gatcggagac ttagagaaaa tcttcactga atccggcaac cttctggtgc aggcctccca
    43441 ttcccaggta ccccaattca gcgccggcga atggaagtct tactccgaaa aaacgaaatc
    43501 agatctcctc atgaactacc ggctcgccta tcgcaaagaa gcttttgtca attggtgcga
    43561 agcactgggg accgttttgg ccaatgatga gatcaaggac gggctgtccg agcgcggggg
    43621 acatccggtg gaacgaaaac ccatgaccca atggtttttg cgcatcaccg cttatgccga
    43681 gcgattgctg caggatttac atgaagtgga attcagtgaa tccatgaaaa tcatgcaaag
    43741 caattggatt ggccggtcca caggggccca gttgtttttt gaactcgagg gtcatcctga
    43801 taaacttgaa atttatacca cgcgggcgga caccattttt ggggccactt ttatggtcct
    43861 ggctcctgag catgaactgg tggggcggat tacgaccggg gagcaggaac aggccgttgc
    43921 ggactacctt cgttatgtaa actcgagatc ggatcgcgac cggatgtctg aggtaaagga
    43981 agtgacgggc gtgtttaccg gcgcctatgc gataaacccg ttcaacgggg tccgaatccc
    44041 ggtatggatt gccgaatacg tgctcaagga atatggcacc ggcgccatta tggccgtacc
    44101 cgcggatgac gccagggaca tggcctttgc caaaaagttt gagct
//
LOCUS       DAOQXT010000030        43927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_971_length_43927_cov_9.116840, whole genome shotgun sequence.
ACCESSION   DAOQXT010000030 DAOQXT010000000
VERSION     DAOQXT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 43927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43927
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_971_length_43927_cov_9.116840"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..400)
                     /locus_tag="PKM27_07155"
     CDS             complement(32..400)
                     /locus_tag="PKM27_07155"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR07075.1"
                     /translation="MKEKITPCLWFDHQAKEAAALYCSLFAKARITAQSPMVTDIEVD
                     GQSITLLDGGPMYKPNASISFFYICESEEEMNRIWNAFLEKGSVLMPLDSYEWSEKYG
                     WINDVYGISWQLSLVYRLLN"
     gene            complement(458..1219)
                     /locus_tag="PKM27_07160"
     CDS             complement(458..1219)
                     /locus_tag="PKM27_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR07076.1"
                     /translation="MEHRYIKVSPEISDYIRTILVLSEGKNSQSHRLPLFTGGMPALM
                     VEAGSDPYSLTLYGQSLPETNWSDRHCSGIILYFFKPYTIGTIFNLTAKSLREPVELS
                     QWNLQKAQALNHRLLHAESMAEKIQILNAFILNEIRDNLRACEIIRLATDHLMQNSKT
                     EDLARVIKELNLTERTFQRIFKKYVGISPNEYRRICQFYFAFSQLKEGQFDKLSDLAG
                     DHGYFDQSHYIRSFKEFTGTTPKGYLKSGLGKEEY"
     gene            1331..1585
                     /locus_tag="PKM27_07165"
     CDS             1331..1585
                     /locus_tag="PKM27_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07077.1"
                     /translation="MITANELKIKGVKAIEEGLKDQPEVAISVRGKVKYVAMTVEQYE
                     QMRAAELEAAYSQVMEDVKAGRYTVETAEEHIARLFNDKP"
     gene            1592..1867
                     /locus_tag="PKM27_07170"
     CDS             1592..1867
                     /locus_tag="PKM27_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07078.1"
                     /translation="MPKLLVTVHYEKKLALFKTAHPYLIKTYAKTILLLAKNPAHPSL
                     RLHKLKGGLSDFYSVSINMKYRIILDFLVQGDQILLIDIGSHGEIYH"
     gene            complement(1876..2790)
                     /locus_tag="PKM27_07175"
     CDS             complement(1876..2790)
                     /locus_tag="PKM27_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNR07079.1"
                     /translation="MNRRSFLAKATLYSAGGYISAKSGNMDDDLIHRTIPSTGEKLPA
                     IGLGSWLTFDVGTSNSELAPVKSVIKSFYEAGGRLIDSSPMYGRSEQAIGESASQLGL
                     TDKLWLATKVWTTGQERGKRQIENSRSLFKTWPMLLQVHNLQDMQSHLRTLRQLKAEG
                     KIKYIGLTHYVNGMHREMANLVRSEKPDFVQINLSIRNTAAEDYLIPTAADHGVAVII
                     NRPFETGALFDAIGGAPLPPWAKDYNIKTWAAYFLKFIISNPDITCTIPATSKEDHVR
                     ENMGACFGLLPDQAGRKRMQEYFFIEAK"
     gene            complement(2821..3453)
                     /locus_tag="PKM27_07180"
     CDS             complement(2821..3453)
                     /locus_tag="PKM27_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNR07080.1"
                     /translation="MNIKNIRTTILLLLLVLVCAPGFAQTKFSGFYAGMLAGSQNIFG
                     GSNVEGIDVLAQKSGFVLEFPVGLRLQTLNKRLVLGLEFSVGMTDGDLLHKDETKPLT
                     INYSNSFQRGFGGTAGWATGRRRNLLLFAYLNETKRKFEVDILDRHGRYSQKDKQGML
                     KYGLGLEFPLAGILHLRASAGLMRVDFGDRITNINVEDRGDVMAGLIFQF"
     gene            3743..4123
                     /locus_tag="PKM27_07185"
     CDS             3743..4123
                     /locus_tag="PKM27_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNR07081.1"
                     /translation="MKQDIRERSDIIHLVDRFYDRVLKNPVIGPIFTDVAHLDFDKHL
                     PVMYSFWASQLLGEQSYSGNPMTPHLKLAALTPMGEIQFNEWLRLFNQTVDELFEGER
                     AEEAKTRAGNIARLMNYKINLELK"
     gene            complement(4364..5647)
                     /gene="thrC"
                     /locus_tag="PKM27_07190"
     CDS             complement(4364..5647)
                     /gene="thrC"
                     /locus_tag="PKM27_07190"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNR07082.1"
                     /translation="MLFYSTKNQEVKEDFVRAIFMGPSKDGGLFMPAQIPALDPDWIT
                     GIEGYTLQEIARKVIAPYIGIASSEISGVVERAFIFPAPVRRVGHHHFLELFHGPTLA
                     FKDFGARFMAQAMSYFRKKDENINILVATSGDTGGAVASAFSGLEGFKVFILFPKNKV
                     SPLQQRQLTTWDDNVIALEVEGNFDDCQRLVKMAFSDQALRNKKIFASANSINIARLL
                     PQMVYYFEAFKQATLKSGLNIIVPSGNFGNLTAGLLAKRMGLPISHFTAATNNNDIIP
                     LYLLTGKYSPRVSRETLSNAMDVGDPSNFVRMLDLYRGRDESSTWNNMKKDLNAHVVD
                     DKTTLQNIREVFERWGYIMDPHTAVAYDAAKMKPGNNIIVSTAHWAKFAETIEKAIGA
                     LPPIPGALSHLMDKTEKVIPIQARYEDLLGVIGGK"
     gene            complement(5809..6717)
                     /locus_tag="PKM27_07195"
     CDS             complement(5809..6717)
                     /locus_tag="PKM27_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR07083.1"
                     /translation="MNESPPGIYQITGFILFSFFPSFCFLQNTCAVVRSPVYPEETRA
                     GLEMELENARKSYERDTANADLLIWYGRRLAYLGRYEEAIEVFSKGMLLYPWDARLYR
                     HRGHRYITVRCFDRAVSDLEYAAGLVRGHKDEMEPDGLPNARNIPTSTLQSNIWYHLG
                     LAYFLKGDYEKAVKAYTSCLEVSGNPDMYVATANWLYISLRRLNRDQEAAKLMNTIEP
                     GMDLIENRDYLDILLLYKGEKSEADFGDRANSLSNASLLFGLGNYYLTMEEFVKAKDI
                     FTKIVSGNQWASFAYIAAGETLKELK"
     gene            6821..7804
                     /locus_tag="PKM27_07200"
     CDS             6821..7804
                     /locus_tag="PKM27_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR07084.1"
                     /translation="MEINWGIIGVGDVCEIKSGPAFNAIPHSRLVAVMRRSSALARDY
                     AQRHGVPKWYDDADALIGDPDINAIYIATPPHVHAYYTQKAAEAGKTVYVEKPMARTH
                     AECLDMVDTCKKAGVPLFVAYYRRALPNFLAIRRWIEDGIIGDIRFVDIQVHKPLMPD
                     IVGASKNPDNWRTDPEIAGGGYFHDLACHQLDMMDFLLGPIEEASGYSLNQAGIYKAD
                     DLVTGHFRFKNGLPGMGSWCFNLSENAQKEVTTIYGSKGTLAFPFFGDHSVTLHVEGR
                     EPENHRFDIPVNIQAPLIQTIVDQVKGVGHCPSTGESGARTNRVMEWIIGV"
     gene            7910..8482
                     /locus_tag="PKM27_07205"
     CDS             7910..8482
                     /locus_tag="PKM27_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07085.1"
                     /translation="MKQEIYFLLILLISLSACNKNDTGQETFDHPSPIKEDLSRSGAI
                     VLKSVKKDFVYFSHSVFTGFPTDFWLYDSISSQWVPRTDFPGKDRLQPIMLLYNDQIF
                     SGLSKVQPLPELNDWFSYDIQNDKWTPKSNFPYSLPSAMTASKSGANILIGCEYPLSD
                     IPLCHFVYQVVGDSWRKSSPPCDSISRSCY"
     gene            complement(8611..11376)
                     /locus_tag="PKM27_07210"
     CDS             complement(8611..11376)
                     /locus_tag="PKM27_07210"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNR07086.1"
                     /translation="MKLIYILLFAFCVFQAGAQTLQIKGRVKDGQNNSLQAASVLLLQ
                     SRDSVMVSFATTDAKGLFSLAAEKNGSYLIQVNFLGFKTGWKAVQTKDSGISLEDILL
                     EDQQTTLPSIEISAEQDLMKIGRDTVEFNAGAFKTQPGAVVEDLLKKLPGVEVQRDGS
                     IRAFGENVQNVLVDGKEFFGRDTRMATKNLEADAVDKVQVFDKKSDMAEFTGVADGRD
                     EKTINLSLKADRKNGHFGNAELAGGSDGRFKTKVNLNRFSPGSRLSLIGLGNNINDQS
                     FSFQEYLDFMGGLGAFMSGGRGRLEINQAGSLPVGGGPVQGVQQSLAGGINFSRDFSA
                     KTSFTASYLANNFKNRLDRNSDRQSLLSDAFYNSLENENQYSRNTGHNITFRLKSKLD
                     SFQNLIINGGGNWGDSRFESLLANSTFLQDYSEVNSSDRKYEADGTRFNFNTSVTWQK
                     RFRKAGRSFVVSASGRYGDNDRQGRLDALNQYFQFGTQADSLLQNQVFEDRNGLMDSR
                     MSYTEPLGRKRFIELNGYWSNARNRTNNDYFDIVDPFREIRNELLSARYNRGYRVYSA
                     GLKYIKNHNKYYLSYGLNLQHSQLNGKIQDLEQTISSGFTRLLPNASLQYELGTAHHL
                     NLEYETRLQEPSLEQLQPVPDNTDPLNIYIGNPGLRPEYVHRMNSSYFKYDQFSFTSV
                     FASLGGQYTRNRITDLIEIDSLFRRTIRPVNVKYEKALQGNLEFSTPVRPLKISTRLR
                     LRAQLAQSLLYVNELENRVNRAGTSVYWSVENRKKDFFDAQAGVRYSRNNTAYSISKD
                     LDQVYKETNLFGELTLIPSSQWSIKSEFDYLSYNQPGRTGRVEVPLWQASLTRFFTKQ
                     QRLKVSLSVFDILNKNQGISRSSELNYLEVQRTNVLGRYFMLGLAYSIKGFKKSGGGI
                     EINIGED"
     gene            complement(11446..12204)
                     /locus_tag="PKM27_07215"
     CDS             complement(11446..12204)
                     /locus_tag="PKM27_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNR07087.1"
                     /translation="MKKDILNLNLILNLLLFLPIWLQAQEAGVIHFTETVKLQIELDE
                     GSRPMMNNLPQSHSVKKILLFKGKESLYKNDTGNEDLDVSNSEGGGEIRMVMRNPESI
                     LYTDLAANSIVHSQEFFGKQFLITGAPQKRSWKITSEQKTLLDMTCQKAVLQDTSMQV
                     VAWFTTQIPAAFGPNGYSGLPGAILSMELDKGIRTITATKIEFREVSPEEIVKPDKGK
                     KVSTEEFEKIRKEKMAEMGAVNGGGGTMKIMIRN"
     gene            12335..13768
                     /locus_tag="PKM27_07220"
     CDS             12335..13768
                     /locus_tag="PKM27_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR07088.1"
                     /translation="MNNYPGRILKPLMWLSLFFLCVFLGVYLKKSYQAEKEALQKEVG
                     YLFINAVKNIEGGLLNRLVFRPDSTDHIIVRHLFDSNDSTQTLDFIGKSSMPLPEGKT
                     RIKIAVSKEKQVSLQETEGMISMIVQMDQDSLSGDSLVRIRPMEASSKIATELRNRFE
                     LNLQAAGLPVKYTFTQFRDTGNLQDDKKDIAGSYFDIASGERYEVALSQYRGLVLKKM
                     APEIILAGLLLLCLGLAFYSMARTIKREKQLLAIKSDFIQNMTHELKTPISTVGVALE
                     ALQDFDAIQDSRKREEYLNISKNELNRLSLLVDRVLSVSGIDKALPPAHPERIDLAAL
                     VNSIADSFRVQTEKQNITIDVHIKDDPLVLHADRQWVAGIVYNLMDNAIKYGNKDGGR
                     VDVFLTREAGQIGIRVKDNGPGIGREYQSRVFDRFYRIPQGNVHTVKGHGLGLAYVRR
                     VVKEMGGKINLESEAGKGAVFTVSLPS"
     gene            13780..14463
                     /locus_tag="PKM27_07225"
     CDS             13780..14463
                     /locus_tag="PKM27_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR07089.1"
                     /translation="MIKLLYVEDEPSLAKIVTETLGSRQFEVRHIGRGDLAMDAFRDF
                     RPDICVLDVMLPYVDGFSLGKEIRSLDSKVPIIFLTAKSQTEDALQGFGSGGNDYIRK
                     PFSMEELIVRIKNLLALSNQSRNLEVSPEALSLGRYLFYAGRQELHLGDSVKRLSHRE
                     TELLSMLIRQKQEAVLRKTILDKIWGNDSFFNSRNLDVYITKLRDYLKEDEKIQIITL
                     KGVGYRLVD"
     gene            complement(14529..15434)
                     /locus_tag="PKM27_07230"
     CDS             complement(14529..15434)
                     /locus_tag="PKM27_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNR07090.1"
                     /translation="MTLKQILPVKPYPRRHFLKLTGFAAGSFFFPGLRRPGQESWTVG
                     QIMDRFIREVPGAPFRITVDTLKSGSRDTVVTGIVTTMFPTIPVIEKTIALKANFIIV
                     HEPSFYNHLDETDWLENDEVFRYKSALLEKHKIAVWRNHDYIHRHQPDGVMAGVLEDL
                     GWTSRYDEESGIIELENPMNLKSLIKQLKKGLDISHLRYIGHVTQSCKKILLMPGASG
                     GRRQIDRIGKTKPDVAICGELQEWETAEYIRDARAKGQTVSLIVLGHIPSEEPGSRYM
                     AQWIRNNIPGMKVTHIPAENPFSYS"
     gene            15528..16214
                     /locus_tag="PKM27_07235"
     CDS             15528..16214
                     /locus_tag="PKM27_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07091.1"
                     /translation="MMYKITRFLVWGFLLLFSCKTAYVPNTVNVPLFDDAGQMRLMAD
                     FNGNIQFAASPGRNFGIMANALFRKDGENTDDLFGKGYLVEAGLGGFKASGPLRWETF
                     AGLGFGKTETRDFGKKFEAKGARFFFQPSVGFHHDIFEIAFTPRLVAGKFQKPETTYT
                     TQELLDNRLYEIDEPVWFFAEPAITGRIGYKWIKLQVQVGRSFKLNDKPLSFEKEMNS
                     IGLILDFGRR"
     gene            complement(16279..16734)
                     /gene="dtd"
                     /locus_tag="PKM27_07240"
     CDS             complement(16279..16734)
                     /gene="dtd"
                     /locus_tag="PKM27_07240"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNR07092.1"
                     /translation="MRALIQRVGKAEVVLEDGTLSGRIGRGILVFLGIENEDREEDIQ
                     YLVHKISQLRIFDDEAGIMNKSVLETGGSILLVSQFTLHANTKKGNRPSYIRAARPET
                     ATPLYGRFIEALKATGIQTETGKFGAMMQVHLVNDGPVTIWLDSKNKDY"
     gene            17259..18344
                     /locus_tag="PKM27_07245"
     CDS             17259..18344
                     /locus_tag="PKM27_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNR07093.1"
                     /translation="MKHSLIPFPVKAWLWTGLVMIFFQILIGGITRLTGSGLSITKWE
                     IVTGTLPPLNRADWEEAFSLYKETPQYQKINRGMSLGEFKWIYFWEYLHRLWARLMGF
                     VFLLPFVFFWAKGYLYPQLKKGLVTVFILAGIVGAFGWIMVASGLIHRPWVNAYKLTL
                     HLGLALVTLSYLWWVYLKTNSPASYPGLGARQNTALIFLVLVCIQILLGGMMSGMKAA
                     LVYPTFPKMNQYWIDPILFRGDSWKWQHFMDYDSYPFLPALVQVLHRITALVLLVLFF
                     TRMYRFFHLKLVRWVALLLVIQVTLGIITLLRSRGFIPVNLGVLHQAAAIVLLLATLR
                     WWFISRAGSGNLEGEQPQGSPTRVSNK"
     gene            18436..19950
                     /locus_tag="PKM27_07250"
     CDS             18436..19950
                     /locus_tag="PKM27_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR07094.1"
                     /translation="MTSTQIEEKPDMGLFWGCFIALIATAFGFILRALLLEDLGNEFN
                     LTNTQKGEISGVGLWPFAISIVLFSLFIDRIGYRAAMIFAFVAQILSAVITIFFAKGY
                     WGLWIGTFILALGNGAIEAFINPVVATMFSKNKTKWFNTLHAGWPGGMVIGGIFALML
                     GPAVSWRVKIGLILIPVLLYGFLLWNKKFPVHERVKAGVTYNEMLREVGMGGAFIILW
                     LIFSQLGQVIGWGGVTSLIIAVLTTLAFGYYIKWAIGQPLYIFLLLIMIPLATTELGT
                     DSWVTDLMGPAMHNIGVQAGWILVYTSLIMSVLRFFSGPIIHKLNPLGLLAVSCVLAI
                     LGLFMLSTSTGIGILIAATIYGVGKTYFWPTMLGIGAEQFPKGGALALNIIGGVGMLG
                     VGIVGTVLLGNVQDRSVVNTLKDMDTKNNTTLSSTYATVEKGSIFGKYMALDGDKVAA
                     AAEPDKTSLTNAASEAKKSALRTVALLPVFTLLCFLALVFYFRSKGGYQAVHLE"
     gene            20060..21238
                     /locus_tag="PKM27_07255"
     CDS             20060..21238
                     /locus_tag="PKM27_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07095.1"
                     /translation="MIDALRKQFNESFTQEKYEAYKQAVAGAAGEAPLFRLAETPVFI
                     PAYFKEKVLRACEDLIDTITRPDFLSASEGAIPSHLQFAGDPGYCPLLIFDFGVCRDE
                     QGTLSPQLIEMQGFPSLYCFQIAQAEACRKVFEIPPGLDNYIGVSKEEYIQLLNKTIL
                     DGADPAETILLDYKPQDQKTRVDFTATKKYLGIDAVCVTDLKQEGKGLYYLKEGKKQK
                     ISRIYNRLIFDEFFAMKIPCVDLRQDLEVSWVDHPNWFCRISKYSLPFITSDFVPDTR
                     FVSDLKNIPEDLQNYVLKPLYSYAGMGVVIDVTKEDLEKIKDPENWILQKKVHYHPAI
                     ITPDIPAKCEIRMMLIWESPDKRPRVVHNLARISKGKMIGVRYNADFSWVGSSTCFFE
                     "
     gene            21244..21687
                     /locus_tag="PKM27_07260"
     CDS             21244..21687
                     /locus_tag="PKM27_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07096.1"
                     /translation="MQIPSLVTSVPDLHTARILAAAGVPYIAFHQKVKNLEEIKPWLE
                     GPRTGMEMKDPDQSLPATDFLIMPASWYDLFSFSGRTIFWKTGDEHFYFGDVVLEKGT
                     LLTPGDNLPGRRFYHYSDYRGPGEENSDTWLDYEEDFNLFESLFL"
     gene            21909..23615
                     /locus_tag="PKM27_07265"
     CDS             21909..23615
                     /locus_tag="PKM27_07265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR07097.1"
                     /translation="MPLSLYFLLLFARSALSFDLPENFLVPGKTVNARIDPQKTDTYF
                     VRMLPGQFSSIRLTEKEVRLYATVYDPQDSLIQLIDENRMGGKEIISIFSKKKGDYKI
                     RVGWNFSKPYSGNYSITLDRLEATGKTPALKAGQLMEGWYENQEPGAAVAVIRDGQPV
                     FVATRGMANLEEKIPITSQSVFELASCSKQFTGLAVAILIDQKKLSLEEDIRTYLPEM
                     PDYGEKITVGHLVYHTSGIRNTDALEMAGFTPEDRITLPMCVKFAANQKSLKFKPGER
                     FNYSNTNYNLLAEIVARVTGQSFSAWTREHMFKPLGMHSTFFKENPGEIYKYKVLSYQ
                     SGREGFRQRPNNWAAAGSSALCTNIDDLVKWVNGFDTKSLITPGVEQLLYAEGSLRED
                     GRTRYAFGNEFRTFDGIREIMHLGLVIGYRTAIVRYPERKLAFIFLSNDDNDAAYQRI
                     PKIRDLFLFGDLRETKPNLKNFPSAETVIKNIEADEKYQEAIDLKPYEGVYCSEEVSA
                     VWNLKKNQERLEIRHPRMDPMLLKNTGPDQFGFIEFSRNESGVVKGLKIRDEGIEFMK
                     MK"
     gene            complement(23641..24405)
                     /locus_tag="PKM27_07270"
     CDS             complement(23641..24405)
                     /locus_tag="PKM27_07270"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR07098.1"
                     /translation="MKIVIIGGTGFIGSRLAAKLRSEGHDVIAASPSSGVNAFTGQGL
                     AEAVEGAYAVVDVSNSSSLDDRAVMHFFQTAGQHLLEAEAAAGIRHHLVLSVVGTDLL
                     QESGYFRAKRVQENLIKESGIPYTIVRSTQFFEFIAVIAQSATRGSTVYLPSAWIQPV
                     AADDVVDTLADLMAGVPLNTTVELAGPEKFRLDDLVRRYLVLNHDPRQIVLDLNATYF
                     GASLSDHTLIPSVNSRIGSKRFDEWMSPMPKINSLL"
     gene            complement(24478..25101)
                     /locus_tag="PKM27_07275"
     CDS             complement(24478..25101)
                     /locus_tag="PKM27_07275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR07099.1"
                     /translation="MTDSWTDKWNKRYETEAFAYGEQANHFLKEQLIKLVPGNILFPA
                     EGEGRNAVFAARLGWKVSAFDISVEGQRKARILAAKNKLSIDYRVGQLQDLDYRDKSF
                     DVLALIYAHFPPEIKSRMHKALDRLVVSGGMVIFEAFSKNHLEYLAADEKVGGPKDLD
                     SLFSVDEISADFDHYDILELREEVIELHEGLYHNGRGSVIRFVGKKR"
     gene            complement(25098..26018)
                     /locus_tag="PKM27_07280"
     CDS             complement(25098..26018)
                     /locus_tag="PKM27_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNR07100.1"
                     /translation="MKAVVYERYGTPEVLEMKNIQKPVPKENEILIKIYATTVTAADW
                     RLRKADPFLARLFNGLFRPKKVKVLGLDLAGVVEETGNKVKSFKKGDAVFAFCGFNFG
                     AYAEYKCLPENQVISLKPSNMSFEEAATVPTGGLTALGFLKKAGIKKGQDLLIYGASG
                     SVGTFAVQLGKYFGARVTAVCSTANMNLVQLLGADRVIDYTKQDYTKLESRFDIVFDA
                     VGKTSKSAGKHLLKPGGKYVSVKGSPGFRPGDLHVLKGLFESGDLKTVIDRRYPLEQI
                     REAHAYAETLRKKGNVVIHILPDTEKIQTP"
     gene            complement(26082..26783)
                     /locus_tag="PKM27_07285"
     CDS             complement(26082..26783)
                     /locus_tag="PKM27_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNR07101.1"
                     /translation="MKTTRTSANLAGVFFIIAAVAAITGALLYDPILKNPDYIIQTRP
                     LEARVLWGAFFEVITALAVIGTPLALYPVLKKYNDRMALACVGFRLLEAAMILVGILS
                     LLAIVSLNHEFIKEAAPNTASYLIAGKSLKALHDWTFTFGPNILLGPSTLMTAYLLFS
                     SRLVPRFISILGLAGGPLIFGCGILVMFGAFSQVSHWAGLLAIPVFLYEMSLAVWLLT
                     RGFNAPAAGPVTVLR"
     gene            complement(26827..27471)
                     /locus_tag="PKM27_07290"
     CDS             complement(26827..27471)
                     /locus_tag="PKM27_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNR07102.1"
                     /translation="MKNSVQTLFILALVFLPSFSFGQFQSSKPAVPKTHLDFFISAVN
                     TELNYGKANPSLKDFKKPAHGVQIGFSLQAGIASRLSLVPELSFIMRGGKLKVNNPLT
                     QDETTLRFYTLELPVLARLHLGRFHLNAGPSLGYNFDGTQKLDGTTTDLSFEKEAGAY
                     KRLDAGLQAGGGLTFRIKQKRAVLDIRYCYGLTNVSYDRESYHRSLLIRLHVSK"
     gene            complement(27838..28533)
                     /locus_tag="PKM27_07295"
     CDS             complement(27838..28533)
                     /locus_tag="PKM27_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR07103.1"
                     /translation="MDNYTCIIIEDEPLALEKTKDFVLKVPFLTLSATFDNALTGLSY
                     LNNNKVDLLFLDINMDELSGIELLESSKISSQVILTTAYQEYALKGYELQITDYLLKP
                     FTFNRFLQAVNKARENLVHRPTEPSPDFIFVKTENRLEKIMISDIVYIEGMRDYRRIH
                     TMNKRVMTLQNFSEFEKLIPPSQVCRVHKSYMVALHQIESIERGRIKIGGELIPISDT
                     YKEDFFQLINSRL"
     gene            complement(28526..29593)
                     /locus_tag="PKM27_07300"
     CDS             complement(28526..29593)
                     /locus_tag="PKM27_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNR07104.1"
                     /translation="MKKSFVVLLHIGFWACYFLMMFIVLGIYSRSVADKADQVARVWN
                     AFYSVLLFAFIPSVISYFIYYFILFPKYLEQKKILPAILLGLLSSGVAAILGYIIIRY
                     FIETGRIIDMDEGGIHGRSTAGRVILVMTTIGALCGTVAFVIRGFVTWYNEIKLKEAL
                     RDKSHAMEMALVKSQLDPHLLFNTINNIDALILKDSVAASEYLNKLSDILRFILYETK
                     GDKIPLAKELEYITKYIALQKIRTANENYIHFTVEGPAGNKSIAPMVFIPFIENAFKH
                     TNNKKLENAIRVQIRIREEDIQFLCENKFDPRPAGQATAGGLGNELIKKRLNLIYGGK
                     HTLEMQKTNDRYLVHLAIPHG"
     gene            complement(29900..30565)
                     /locus_tag="PKM27_07305"
     CDS             complement(29900..30565)
                     /locus_tag="PKM27_07305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNR07105.1"
                     /translation="MDKQIFGQVDQYISDLLLPPDPVLDDTLKSIENAGLPAISVSPV
                     QGRFLQLMVLLTGARRILELGTLGGYSTICLARALTEKGRLISLELDPRCAEVARGNI
                     NKAGLGAKVEIRVGKALDLLPGILASNEGPFDLIFIDADKPPYAEYFDYAVKLSRPGT
                     LIICDNVIREGKVLDPRCEDELVQGVQRFNKALARDHRVTATLFQTIGAKEHDGMALA
                     VVK"
     gene            complement(30691..31929)
                     /gene="dinB"
                     /locus_tag="PKM27_07310"
     CDS             complement(30691..31929)
                     /gene="dinB"
                     /locus_tag="PKM27_07310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNR07106.1"
                     /translation="MYDRAILHMDLDSFFVSVECLRNASLKGKPLLIGGSGDRGVVAS
                     CSYEARRFGIHSAMPMKMARRLCPDALILRGDMDAYSEYSRQVTELISGEAPCFEKAS
                     IDEFYLDLSGMDRYFGCYQWGVDLRKRLIREIGLPISMALSVNKTVSKIGTGEAKPNG
                     SKEILKGTEKDFLAPLSTSKMPGIGEAMYKKLSFMGVRTLRVLSEIPPRLLEREFGKT
                     GRVLWERANAFDPTPVQPYSEAKSISHEQTFSQDTLDLRRIRNILLSMVEKLGFDLRQ
                     SKKLCSVVTVKIRYADFNTYTRQLRIPYTANDRILLRHVYDLFDKLYERRQMIRLIGV
                     KFSGLVHGHYQINLFDDTEEDMNLLNELDHIRKRWGPHAIGRAVNVDRKKEEDHQTQP
                     SKTQTAVTDPRWAKMVRRWW"
     gene            32275..32817
                     /locus_tag="PKM27_07315"
     CDS             32275..32817
                     /locus_tag="PKM27_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNR07107.1"
                     /translation="MNEELSKVTIHLVSSLDGFIAKKDNSVSWFETTDYYEKGVTGED
                     EEEFLKTIGCYVIGAHTYEHTLEIGWPYGETPVTVVTHRNLRHNGKNIEFYSGSLEKL
                     VNAHLKPKYRNIWIAGGAMLAEDIIRLNLADDLRLSILPVMLGDGKRFLDHIGREEAL
                     HLKDVTAYKSGMVELWYEIR"
     gene            complement(32821..33225)
                     /locus_tag="PKM27_07320"
     CDS             complement(32821..33225)
                     /locus_tag="PKM27_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07108.1"
                     /translation="MRFLFFSLLGGFFLLACSKPGADPVLDCPGEGRVQAELIRKGCE
                     AYVFRLIEYSPDAQEKWTDIFTKKEYSNVISVLNYCGDDQNSKDLQALNAGEFVSFNL
                     TATQEHCAILCLVYEASPAPTFKASKVSRCSN"
     gene            complement(33265..34302)
                     /gene="porQ"
                     /locus_tag="PKM27_07325"
     CDS             complement(33265..34302)
                     /gene="porQ"
                     /locus_tag="PKM27_07325"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNR07109.1"
                     /translation="MKSGHRFLIFLFFSFGPLSIFAQNGGRAIYEFLQWGPSARVTAL
                     GGRYISARDSDVVLAQMNPAVLSFQSTGTIAVNQSFYLGGTAYSNLASAFHLSKENLT
                     IHGALQYLNYGRFQGADEFGNKTNEFKARDMAIVAGASKKLFESLSIGLNLKFIFSQL
                     ETYNSSGLGLDLGLLYALKSGRQSFGLSFRHMGVQWDPYWETRENLPFNIEMGFTQRL
                     EHVPILFSITAHDLHRWDLRYDSPDEETNLFGEPVGRPSAVERFVDNLARHLIFSSEV
                     LIGKNAPLRLRLAYHHQRHQELKDQNYRGLSGFSGGLGLKLSRLSFDYGLANYHLAGS
                     MHSIGLGIRVK"
     gene            34497..34910
                     /locus_tag="PKM27_07330"
     CDS             34497..34910
                     /locus_tag="PKM27_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR07110.1"
                     /translation="MKRVTGLGGFFFKTQDATSLKSWYSKHLGLVTDEYGATFSWHKE
                     NGQKGYTVWSLFKEDTTYFNPGTKPFMINFRVENLEELLKTLKEEGVQVVGDMEVYEY
                     GKFGWILDPEGNKIELWEPHDNEFGKILPSENPST"
     gene            35002..35394
                     /locus_tag="PKM27_07335"
     CDS             35002..35394
                     /locus_tag="PKM27_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR07111.1"
                     /translation="MKNAISWFEIPTTDLDRATAFYETIFQVKLNPLDLANIKMRMFP
                     LEDIMGVGGALTYAEGFYKPSATDGPLIYLNANPDVQIVLDRIPEAGGTILVPKTEIS
                     PEYGFMAVFLDSEGNRIALHSVPAIPGA"
     gene            36059..37213
                     /locus_tag="PKM27_07340"
     CDS             36059..37213
                     /locus_tag="PKM27_07340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR07112.1"
                     /translation="MRLYKQIASILLLTALLLELRAQETYYPDSTWARKKPQELNMNN
                     EGIEEAVRFAMANETHTDKNLSAAMYKAYEREPGYSILGPVRPRGGPAGLILKNGYIV
                     AEWGDLQRVDMTFSVTKSYLSTLAGLAADSRLIRSIDDPVRGYVWDEKFEGPHNSKIT
                     WRHLLNQSSDWSGCLFDICDWADRPPSSGTPDEWKSRKLLEPGSQFEYNDVRVNLLAY
                     ALLQVWRKPLPVLLKEKIMDPIGASSTWRWYGYENSFVNVDGLMVQSVSGGGHFGGGL
                     FINTLDQARFGLLFLRKGKWKNQQLFSEKWINAVRQPSAPNPSYGLMWWTNEKNELAG
                     VSKNIYYANGFGGNYIVIDPEHDLVIVTRWLDTGKLGELVKRVIDAVESK"
     gene            complement(37255..39435)
                     /locus_tag="PKM27_07345"
     CDS             complement(37255..39435)
                     /locus_tag="PKM27_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNR07113.1"
                     /translation="MNNKKEGRAHPMSRRQFMLTAGGFSFAVTAGAIAPRFLGDPEPA
                     ASSGDQQLTAWVKITADGKITIYNPAAEMGQGSMTALAAILAEEMDADWDKVTIEYSP
                     VEPGIYGMQWNGTPGGPMITAGSRTVRGYYKPLRQAGAKARYVLLYNAAKKWGLPQSG
                     LKTDRGYVIDPAGGRKLSYGEIASFAQIPPSVPEINEQDLKKPEQFRLIGQIIPRYDI
                     PEKTTGRAKFGIDFSLPDMVYAAIARAPVYGSTPELLNENEVRTSDGLVDLVKMDHGI
                     GVVANTFEQALKAKKRMQIRWSGEARAKGYDSVEDYKSLELLIRDENKKGRMVVDTGD
                     APSNIQSAYKIYEREYRTDYLYHAQMEPLNAVIHIAADGKSAEAWAGTQAPDTERNAI
                     AKVLGLDESKVTFHNCYSGGGFGRRQEDFSLEAAMLAKQVRRPLKLIWTREDDIQYGA
                     FRPMSLQRMEAGVDRDGHLTGWQHRIAGPDNRLQTGGVRMNYYTIPNQQTEHRVSHPG
                     VRTAFWRSVGHGPNKFAQEAFIDEIAHDLQIDPYEYRRRMMKDLPRHLALLDKAAALS
                     GWGKSPAPGRAYGMAFSDYGGSYTSGVVEISLDQESGQIKVHKVWAAVDAGVVVQPDN
                     AIAQMEGGIVMGISSVLQESITFKNGQVQQSNFHDYPILRMADCPESIEISLMPSSLP
                     PTSLGELSLPLMGGAITNAFLKLTGKALRHMPFTPAKVLEVLSK"
     gene            complement(39432..39893)
                     /locus_tag="PKM27_07350"
     CDS             complement(39432..39893)
                     /locus_tag="PKM27_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR07114.1"
                     /translation="MKTSFKLNGKMVQVETAPDATLLWTLRDALQLTGTKYGCGASLC
                     GACTVHLDGIAVRSCMTPVSSVAGRSVTTIEGLAGEELHPLQKAWIEEQVPQCGYCQS
                     GQIMQAAALLKANPHPDRELIISYMNGVLCRCGTYTRILKAIEKVSKSGKQ"
     gene            complement(40002..40676)
                     /locus_tag="PKM27_07355"
     CDS             complement(40002..40676)
                     /locus_tag="PKM27_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07115.1"
                     /translation="MDQVPEFVSAFFILTALLTLYLFFRAAFNSRIVVFILLAWLSLQ
                     GFIASTGFYTLTDAFPPRLLLGILPPLVFIVVLFVSPKGRLFIDGLDLKALILVHMVR
                     IPVELVLFWLFSFGTIPQLMTFEGRNFDVLSGISALPVYYFGFIKKSLDIKFILAWNY
                     ICLALLANIVIHALFSFPYPFQLLAFDQPNIALLYFPFLWLPAVVVPLVLFSHLAAIR
                     QLRRKQ"
     gene            40772..41323
                     /locus_tag="PKM27_07360"
     CDS             40772..41323
                     /locus_tag="PKM27_07360"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR07116.1"
                     /translation="MIPTFNPGIRFALPADAASIVALLNSAYRGEGSKQGWTTEAHLI
                     AGDRRTDIPHLIEIMARPDSVFLVYQDGDGPITACVNLQRQGNKLYLGMFSVSPQMQG
                     KGIGKQLLKAADEYARHLGCTAIYMSVISVRDELIRWYQRHGYKDTGERKPFVEDGLT
                     GKHLRPLEFMTLEKEVLSSPAPS"
     gene            complement(41439..42209)
                     /locus_tag="PKM27_07365"
     CDS             complement(41439..42209)
                     /locus_tag="PKM27_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNR07117.1"
                     /translation="MKRSVNKLAGLLLLLAGYSTPALGQNTRISDPNTIGWYTNTTTV
                     AFTKRFSGHAEFQWRRDQLIKTWQQGVYRFGMNYTINPKLTLRAGYGFIETFNYGDYP
                     IQAAGKTFPEHRIYQLASLNDQNGRFEMNHRFILEQRWTGRFTNPSFKYADEYVFTHR
                     FRYMFRIQTALGKKAMTDHTPYLALYDEILLGFGKNVNENVYDQNRIGALLGYRFNKT
                     FRLEGGFVQQLLQLGREINGRNVFQYNNGVIVNTILNL"
     gene            42359..43885
                     /locus_tag="PKM27_07370"
     CDS             42359..43885
                     /locus_tag="PKM27_07370"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNR07118.1"
                     /translation="MKTIKLAIWIVAMHGWVITQAQNAFDRDDTLRGSITRARAWWDL
                     KYYRLEIEPKPESKTLSGKVDIHYEVLFPGNLLQIDLQPPLAIEKIIQNGENLDFIKD
                     GENAHLVTLKKNQVRGSRQVLEVYYSGAPKEAKNPPWQGGVQWTRDQDGNHFIATSCQ
                     GLGASAWWPCKDHMYDKPDSVSISVTTPPGLMDVSNGKLRSVTAHPDGRRTFEWFVSN
                     PINNYGVNMNIGRYVHFSDTFHGEQGILDLDFYVLPYNLEKAKEQFKQAKLMLRAFEH
                     WFGPYPFYRDGYKLVETPYLGMEHQSSITYGNGFANGYRGTDLSGTGWGLKWDFIIIH
                     ESGHEWFANSITYKDIADMWVHESFTNYSENLFTEYYYGKEAGSDYVIGTRKKIRNDK
                     PIIGTYNVNRSGSGDMYYKGGNMLHTIRQMVNNDEKWRRILRKMNQQFYHKTVDGSEI
                     EKFMSKETGKDLSKIFDQYLRDTRIPTLEYTLENNKLTYRWTNAVPGFNMPVRIHLVP
                     "
BASE COUNT        11459 a        10547 c        10696 g        11225 t
ORIGIN      
        1 ataaatgaat gtttacgaca aatatactaa tttaatttag taatctatac actagagaca
       61 attgccagga aatgccatac acatcgttta tccaaccgta tttttcactc cattcatagc
      121 tgtccagggg catcaggaca gaaccctttt ccaggaaagc attccagatg cgattcattt
      181 cttcttcgga ctcgcagata taaaaaaagg agatggaggc attgggtttg tacatcgggc
      241 cgccgtcgag cagggtgatg ctttgtccgt caacttctat gtcggtgacc atcggcgatt
      301 gggccgttat cctggctttc gcaaaaagag agcaataaag cgctgcggct tcttttgcct
      361 gatggtcaaa ccaaaggcag ggcgtgattt tttctttcat ttttggagtt ttggacaaaa
      421 ttatccgggg agtgaggcga ttcattgtat aaaaacgtca atattcctct ttccccagac
      481 ccgacttcag atatcctttt ggcgtcgtcc cggtaaattc cttaaacgag cggatataat
      541 ggctttgatc aaaataccca tgatcaccgg ccaggtcgga taatttgtca aactggcctt
      601 ccttcaattg ggaaaaggca aaataaaact ggcagattct tcggtattca ttggggctga
      661 tgcccacata ttttttgaat atcctctgaa aggttctttc agtaaggttt aattccttga
      721 tcacccgggc caggtcctcc gtttttgaat tttgcatcag gtggtcggtg gccaaacgga
      781 tgatttcaca ggctctcagg ttgtcccgga tctcgttcaa aataaaagca ttcaggattt
      841 gaattttttc tgccatggat tcggcatgca ataaccgatg gttgagcgct tgcgcttttt
      901 gaaggttcca ttgactgagt tctaccggtt ctctcagtga ctttgcggtc aaattgaaaa
      961 ttgtgcctat ggtatacggt ttgaaaaaat aaagaataat cccggaacaa tgccggtcgg
     1021 accaattcgt ttccggaagc gattgtccat aaagagttag cgaatatgga tcgcttcccg
     1081 cttcaaccat caaggcgggc atgccccccg taaacagggg cagcctatgg ctttgtgaat
     1141 tcttcccttc gctcaaaacc aggatggtcc ggatataatc gcttatttcc gggctgactt
     1201 taatgtaccg gtgctccatt ttttatctaa aaccgactgg tccaagttcc ctcttttttc
     1261 aaaaattaaa ccgtatattt gaccgtacga ttaaccgtac tgtttttcgc accattaacc
     1321 gcattgtttt atgattaccg ctaatgaatt gaaaatcaaa ggagtaaaag ctattgaaga
     1381 gggtttaaaa gaccagccag aggttgctat cagtgtccgg ggtaaggtaa aatatgtggc
     1441 catgaccgtg gagcaatacg aacaaatgcg cgcggccgaa ttggaggcag cctattcgca
     1501 ggtcatggaa gatgtaaaag cgggaagata tactgtcgaa accgctgaag aacatatcgc
     1561 caggttattt aatgataaac cctgaagccg aatgcctaaa cttcttgtta ctgttcatta
     1621 tgaaaaaaaa ttagccttat ttaaaacagc acatccttat ttgataaaga cgtatgcaaa
     1681 aaccattctg cttcttgcca agaatccggc acatccttcg ctcaggctac ataaattaaa
     1741 aggaggatta tctgattttt attcagtttc catcaatatg aaatacagga taatccttga
     1801 tttcctggtt cagggggatc agatcctctt aattgatatc ggcagtcatg gagaaatata
     1861 tcattgaaaa tttaatcact tcgcctctat aaaaaaatac tcctgcattc tcttcctccc
     1921 agcctgatcc ggcaataagc caaaacaagc ccccatgttt tccctgacat ggtcttcctt
     1981 tgaagtcgcc gggatggtgc aggtgatgtc cggattggaa ataatgaatt tcaggaaata
     2041 agctgcccag gttttgatat tgtaatcctt cgcccagggt ggaagtggcg cccctccgat
     2101 cgcgtcaaac aaggccccgg tttcaaaagg gcgattgatg atgacggcta ccccatggtc
     2161 tgcggctgtc gggatcaaat agtcttccgc cgcggtattg cgtatggaaa gattgatctg
     2221 tacaaaatcc ggtttttcgg acctgaccag gttggccatt tcacgatgca tgccgttgac
     2281 ataatgggtc aggccgatgt attttatttt accctcagct ttgagttgcc gcagggtccg
     2341 tagatggctt tgcatatcct gcagattgtg gacctgcagg agcataggcc aggttttaaa
     2401 caagcttcta gaattttcaa tctgtcgttt ccctctttct tgcccggtcg tccagacctt
     2461 ggtggccaac caaagtttat cggtaaggcc caattgggat gccgattcgc caatggcctg
     2521 ttccgaacgc ccgtacatgg gggagctgtc gatcagtctt cctccggctt cgtaaaacga
     2581 tttgatgacg gatttaaccg gggcaagttc agagttggag gtacccacat cgaaggtcag
     2641 ccaggagccc aggccaatgg cgggaagttt ttcaccggta gacggaatcg ttctgtgaat
     2701 caggtcgtcg tccatatttc ccgatttggc ggaaatatac cctcctgctg aataaagggt
     2761 ggcttttgca agaaaggatc tcctgttcat tttaaacttt ttgcaggact tgttccctgt
     2821 ttaaaattga aagataaggc ctgccatgac atcgccccgg tcctctacat tgatattggt
     2881 gatccggtct ccaaaatcca cccgcatcaa tcctgcggag gcccggagat gcaagatccc
     2941 ggccagcggg aattccaggc ccaatccgta tttgagcatt ccctgtttat ctttttggct
     3001 gtatctaccg tgcctgtcca ggatgtccac ttcaaatttt cgtttggttt cattcaggta
     3061 ggcaaacagc aaaagattcc gtctacggcc agtcgcccat cctgccgtac cgccaaaacc
     3121 cctttggaaa gaattggaat agtttattgt cagcggtttt gtttcatcct tgtgcaataa
     3181 atccccatcg gtcatgccca cggaaaattc aagccccaaa accagtcttt tattcagggt
     3241 ctgcagtcgt aatcccacag ggaattccaa tacaaagccg gatttctggg caaggacgtc
     3301 aatcccttcc acattggagc ctccgaaaat attttgtgat ccggcaagca tcccggcata
     3361 aaatcctgaa aattttgtct gggcaaaccc tggagcgcaa accagcacca gcaaaagcaa
     3421 taggatcgtt gtcctgatat tttttatatt catctgattc attaattttt tgaaaatcag
     3481 tcttcttccg tacggacatt ggtccggaaa acaagacatg gacttaatga atctggaagg
     3541 cgctttttca aagaatggta ttgctttgaa cggccacttc aaaaataaac gaaagccctt
     3601 tatcctgtgt ttgttttttt agccccctgg cggagtacaa cgacattcta aaggaaaatc
     3661 ttcgaaaaat cgttgcagtc agcacattat ttgttgttat tttaccacaa taccacaccg
     3721 tgactaaact tggaggcggc ttatgaagca agacatccgg gaacgttccg atattattca
     3781 tttggttgac cgtttctatg accgcgtact gaaaaacccg gtgattgggc ccatttttac
     3841 cgatgtcgct catctggatt ttgacaaaca cctcccggtc atgtactctt tctgggccag
     3901 ccagttgctc ggagaacaat cttattccgg caatcccatg acgccacacc taaaactggc
     3961 tgccctgacc ccgatggggg aaatacagtt taatgaatgg ctgagattgt ttaaccaaac
     4021 cgtggacgaa ctctttgagg gggaaagggc ggaagaggcc aagacccgcg cggggaatat
     4081 agcgaggctt atgaattata agatcaacct ggagttgaaa taagcacacg gagtattggt
     4141 ttaacttagg gtttcggcat ctgtcagggg tttatcaatc atctataaga acccattgat
     4201 tctgatggcg cacagagcaa gggagagcac ggggattttt atggggaggc attagtaaac
     4261 taccaaacac ttttggctgt tgcgcaaact ttgacctcct ttgggcaaat aaacatgtaa
     4321 agtctccgtg ctctccttct ctccgtgaga ggccaaacag ctcttacttt cccccgatta
     4381 cccccaacaa atcctcatac ctcgcctgaa ttggaattac tttttcggtc ttatccatta
     4441 aatgactcag tgcgccgggg atcggcggaa gagctccaat ggccttttct attgtttcag
     4501 caaacttggc ccaatgggct gtggacacaa taatattatt gcccggtttc atcttcgccg
     4561 catcgtaggc caccgccgta tggggatcca tgatataccc ccaccgctca aaaacttccc
     4621 ggatattttg cagggtggtt ttatcgtcga ccacatgggc gttcaggtcc tttttcatat
     4681 tgttccacgt ggaactttca tcgcggcccc gatacaaatc cagcatccgg acgaaattac
     4741 tgggatcccc cacgtccatg gcattggaca atgtttcacg tgaaacacga ggggaatact
     4801 taccggtaag aagatatagg gggataatgt cattattatt ggtagcggcg gtaaaatggg
     4861 aaatgggaag ccccatacgc tttgccaaaa gtcctgcggt taagtttcca aaattacccg
     4921 aggggacaat aatattgagg cccgatttta aggttgcctg tttaaaagct tcgaaataat
     4981 aaaccatctg gggaagcagg cgtgcaatat tgatggagtt ggcagaagca aagattttct
     5041 tattcctcaa cgcctggtcg gaaaaagcca tttttaccag cctttgacaa tcgtcaaaat
     5101 tcccttccac ttccagagcg attacattat catcccaggt tgtgagttgc ctttgctgta
     5161 gcggacttac tttgttcttc ggaaaaagaa taaatacttt gaaaccttcc agcccactga
     5221 aagcgctagc taccgctcct cccgtatctc ctgaggtagc aaccaggata ttaatgtttt
     5281 catctttttt acggaaataa ctcatcgcct gagccatgaa ccgggcgcca aaatctttaa
     5341 aagccagggt aggaccgtgg aaaagctcta aaaaatgatg atgtccgact ctcctgaccg
     5401 gggcgggaaa aataaaagcc ctctccacaa caccacttat ttcagaagag gcaataccta
     5461 tataaggagc gatcaccttc ctggctattt cctgaagtgt gtatccttct atacccgtga
     5521 tccaatccgg atccagggct ggaatttgtg caggcataaa caagcccccg tctttagagg
     5581 gccccataaa tattgctcta acgaaatcct ctttaacctc ttgattttta gtactataaa
     5641 ataacatgag taacaaaaaa catattatat taaataactg gcgcgatata cggataaatt
     5701 acagaaataa atttgctatt tatactaata aacagtcttt tatatattaa attttgaaat
     5761 atctaaaata aagttagact tagactgaaa aatcaaggtc ccaatacatt attttagttc
     5821 tttcagcgtt tccccggccg caatataagc aaagcttgcc cattgatttc cggatacaat
     5881 ctttgtaaaa atgtccttcg ccttaacaaa ctcctccatg gtcaaataat aattgcccaa
     5941 cccaaacaga aggctcgcgt tggataaaga atttgcccga tccccaaaat ctgcttcact
     6001 tttttctccc ttgtataata aaagaatatc caggtaatcc cggttttcga tcaaatccat
     6061 ccccggctca atggtattca tgagcttggc ggcctcctga tctctgttta atctccgaag
     6121 actgatatac aaccagttgg cagtggccac atacatatcc ggatttcctg aaacctccag
     6181 gcaggaggta tacgccttta ccgctttctc gtagtccccc ttgagaaaat atgccaatcc
     6241 caaatgatac catatattgg actgaagcgt actggtgggg atattcctgg cattgggaag
     6301 cccatccggt tccatctcgt ccttatgtcc ccggaccagc ccggcggcat attcaagatc
     6361 cgataccgcc cggtcaaaac aacgaaccgt aatatacctg tgcccccggt gcctgtacag
     6421 tctggcgtcc cagggataca aaagcattcc ttttgaaaaa acctcaatgg cctcctcata
     6481 cctacccagg taggccagcc ttcttccata ccagatcagg aggtcggcat tagcagtatc
     6541 ccgctcataa gatttccggg cattttccaa ttccatttca aggccggccc tggtctcttc
     6601 cgggtaaacc ggggatcgta caacagcgca tgtgttttgc aaaaaacaaa aggaaggaaa
     6661 aaatgaaaac aggataaaac ccgtaatttg ataaatacct ggggggcttt cattcataca
     6721 aaatattgat tttaatctaa ccctagcagg accttgactt tgtctttgcg ccttcgcgcc
     6781 tttgcgagaa atatccccaa accctacctt agcaaacatt atggaaatca actggggcat
     6841 catcggcgta ggcgacgtct gtgaaatcaa aagcggccct gcttttaacg ccataccaca
     6901 ttccaggctg gtcgctgtca tgcgtcggtc atccgccctg gcccgggatt atgcccagcg
     6961 ccacggagtc ccgaaatggt atgatgatgc ggacgccctg atcggcgacc cggacatcaa
     7021 cgccatttat atcgcaaccc ctccgcatgt acatgcctat tatacccaaa aagcggcaga
     7081 agcgggaaag acggtctatg tcgaaaaacc catggcccga acccatgccg aatgcctcga
     7141 tatggtcgac acttgcaaaa aggctggagt cccacttttt gtggcttatt accgaagggc
     7201 cctgcccaat ttcctggcca tccgtcgctg gatcgaagac ggcataatag gggatatccg
     7261 ttttgtggat attcaggtgc acaaacccct catgccggat attgtgggcg ccagtaaaaa
     7321 tccggacaac tggcgtacgg acccggaaat tgcaggggga gggtattttc acgacctggc
     7381 ctgccatcaa ctcgatatga tggatttctt attaggcccc atagaagagg cctccggtta
     7441 cagtcttaac caggcaggaa tctataaagc ggatgacctg gtaactgggc atttccggtt
     7501 taaaaacggc ctcccgggta tggggtcctg gtgttttaac ctcagcgaaa atgcccaaaa
     7561 agaggtcacc accatttatg gaagcaaagg caccctggcc ttcccctttt tcggagacca
     7621 ttccgtgacc cttcacgtgg aaggacggga accagagaac catcgttttg acatcccagt
     7681 taatatccag gctcccctga tccagaccat cgtggaccaa gtcaaggggg tgggtcactg
     7741 tcccagcacc ggggaatcgg gggccaggac aaaccgggtg atggagtgga taataggagt
     7801 ataggattaa taggattgat aggattgata ggattgatag gattataaga tttgtgggat
     7861 ttgtgggatg ataattgggg ggcaatgctc ctgtttaatg ataaccaata tgaaacaaga
     7921 aatatatttt ctgctgattc tattgatttc gctttcggcc tgcaataaaa acgataccgg
     7981 tcaggagact tttgaccacc ccagtccgat aaaagaagac ctatcgcgtt ccggggccat
     8041 tgtgcttaaa tccgtcaaaa aagactttgt ctatttcagt cattccgttt ttaccggttt
     8101 tcccactgat ttctggctgt acgacagcat ctcttcccaa tgggtgcccc ggaccgactt
     8161 tcctggaaag gaccggcttc agcccattat gctcttatac aatgaccaga tcttttcggg
     8221 cctaagcaaa gtccagcctc tgcctgaact gaatgactgg ttttcatacg atatccaaaa
     8281 cgataagtgg actcccaagt caaattttcc ttattctctt ccttccgcca tgaccgcgtc
     8341 caaatccggg gcaaacatcc tcatcggatg cgaatatccc ctatccgata tccccttatg
     8401 ccattttgtt taccaggtgg tgggagattc ctggaggaaa agttctccgc cctgtgattc
     8461 gatcagcaga agttgttatt gactaggatt agtaggattg ataggattaa taggattagt
     8521 aggattagta ggattgatgg gattgatggg attgatggga tattgttacc tttttcctga
     8581 ctactgacta ttgactattg actactgtgc ctaatcctcc ccaatattta tctctatccc
     8641 tcctcccgat tttttaaatc ctttgatgga atatgcaaga cccagcataa aatacctgcc
     8701 cagtacattg gtgcgctgca cctccaggta attaagttcg ctgctccggc taatgccttg
     8761 gtttttgttc agaatatcaa aaacggacag gcttacttta agcctttgtt gcttggtaaa
     8821 aaaacgggtg agactggcct gccagagagg gacttccacc ctgcccgtac ggcccggctg
     8881 gttataagac agatagtcaa attccgattt aatcgaccac tggcttgagg ggatcagggt
     8941 taattcaccg aaaagattag tttccttata aacctggtcc aggtctttgc tgatcgagta
     9001 ggccgtgtta tttcggctgt agcggacgcc tgcctgggca tcaaaaaaat cttttttacg
     9061 gttttccact gaccagtaaa cagaagttcc cgcacggttg accctgtttt caagttcatt
     9121 cacataaagc aggctttggg ccagctgtgc ccggaggcgc agacgggtgg aaattttcag
     9181 gggccttacc ggggtgctga actccagatt tccctgcaag gccttttcgt acttaacatt
     9241 aaccggccgg atcgtcctcc tgaacaaaga atcaatttcg atgaggtccg taatccggtt
     9301 ccgggtatat tgtcccccta ggctcgcaaa tactgaagta aaggaaaact ggtcgtattt
     9361 gaaataggat gagttcatgc ggtgtacata ctccggacgg agtccgggat ttcctatata
     9421 gatattgagc gggtccgtgt tgtcgggcac gggttgtagt tgttccaatg aaggttcctg
     9481 gagccttgtt tcatattcca ggttgaggtg atgcgccgtt cccaattcgt attgcaggga
     9541 agcgtttgga aggaggcggg taaatcccga agaaatggtt tgttccaggt cctggatctt
     9601 tccgttgagc tggctgtgct gcagatttag tccgtaagag aggtaatatt tattatggtt
     9661 tttgatatac ttcaagcccg cgctatagac ccggtaaccg cggttgtagc gggcggataa
     9721 gagttcgttc cggatttccc ggaacggatc gacgatatca aaataatcat tattggtccg
     9781 gttccgggca ttggaccagt atccgttgag ctcaataaac ctttttctac caagtggttc
     9841 ggtataggac atcctggagt ccattaagcc attgcggtcc tcaaaaacct ggttttgcaa
     9901 taaactgtcc gcctgggtgc cgaattgaaa atattgattc agggcatcca gtctgccctg
     9961 ccggtcgttg tccccatacc ggcctgaagc gctgacgaca aaagacctgc cggctttcct
    10021 gaaccttttt tgccaggtga cggaggtatt aaaattaaac cgggttccat ccgcctcata
    10081 tttccggtcg ctgctgttaa cctccgaata gtcctgaaga aaggtcgaat tagccagcag
    10141 gctttcaaaa cggctgtcgc cccagttgcc gccgccgttg atgatcaggt tctgaaaaga
    10201 atccagtttt gatttcaggc ggaaggtgat gttgtgcccg gtattccggc tgtattggtt
    10261 ttcattttcc aggctattgt aaaaagcatc actcagaagg ctttgcctgt cggaattacg
    10321 gtccaggcgg tttttaaaat tattggccag ataactcgcg gtaaaactgg tttttgcaga
    10381 aaaatcccgg gaaaaattaa tccctcccgc aagagactgt tgaaccccct gcaccggccc
    10441 accgcccaca gggaggcttc ccgcctggtt tatttccagc ctgccccggc cgccgctcat
    10501 aaaagcgccc aagccgccca taaaatcgag gtattcctga aaagaaaaac tctggtcatt
    10561 gatattattc cccaggccaa tcagcgaaag cctggaaccc ggcgaaaacc ggttcaggtt
    10621 aacttttgtc ttaaaccgtc catcagaccc cccggccagt tccgcattgc caaaatggcc
    10681 attcttccgg tcagctttaa gtgaaaggtt tatggttttt tcatcccggc catcggccac
    10741 tccggtgaat tcggccatat ccgatttttt atcaaaaacc tgcaccttgt ccacggcatc
    10801 ggcttccaga tttttagtag ccatccgggt atctcttccg aaaaattcct tgccgtcaac
    10861 caatacgttt tgcacgtttt cgccaaaagc ccggatggac ccgtcccgtt gaacctccac
    10921 tccgggtagt tttttaagta ggtcttccac cactgctcca ggctgggttt tgaacgcgcc
    10981 cgcgttaaac tccaccgtat cccgtccaat cttcatcagg tcctgttcgg ctgaaatttc
    11041 tatggaaggc agggtggttt gctgatcttc aagtaggata tcttccaacg atattcctga
    11101 atctttggtc tgaacggctt tccagcctgt cttaaaaccc agaaaattga cctggatcag
    11161 gtagctgccg tttttctctg cggcaaggga aaataagccc ttggcgtccg tggttgcaaa
    11221 acttaccatc acggaatccc gggattgcag aagtaacacg gaagccgcct gcaggctgtt
    11281 gttttgcccg tctttcaccc ttcccttgat ctgcagggtc tgggccccgg cctggaaaac
    11341 gcaaaaagca aaaaggagaa tatatataag tttcattgcc tactaactac taactattga
    11401 ctactgacta ttgactactg actactgact attgactatt gactattagt tcctgatcat
    11461 aatcttcatc gtaccacccc ctccgttgac cgctcccatt tcggccatct tttctttccg
    11521 gattttttca aattcttcag tgcttacctt tttgccttta tcgggtttca cgatttcttc
    11581 cgggctgact tccctgaact cgattttcgt agccgtaatc gtgcgaattc ccttatccag
    11641 ttccatggaa agaatggcac cgggaagccc gctgtacccg ttagggccga aggcggcagg
    11701 aatctgggtg gtaaaccagg caaccacctg catggaggta tcctgtaaaa ccgctttctg
    11761 gcaggtcata tccagcaggg ttttttgttc gctggtgatt ttccaggacc ttttttgcgg
    11821 cgccccggta attaaaaatt gtttgccgaa aaactcctgg gaatggacga tcgaatttgc
    11881 ggcaaggtcc gtgtataaaa tactctccgg gtttctcatg accatccgga tttcccctcc
    11941 cccttccgaa ttgctgacgt ccaggtcttc attgcccgta tcgtttttgt acagggattc
    12001 tttgccctta aacaacagta tttttttgac ggagtggctt tggggcagat tgttcatcat
    12061 agggcggctg ccttcatcca gttcgatttg aagttttacc gtctcggtaa aatggatcac
    12121 gccggcttcc tgggcttgca gccatatagg aagaaacaaa agaaggttga ggatcaggtt
    12181 gaggttgaga atgtcttttt tcataaaagc gatttttatg aaagcaaaag tagaacgact
    12241 cctcgccagc gcaggttaat caaggtagaa tcaaggttaa ttaaggttaa tcgccagaaa
    12301 accgtgccct tttgaggctt atttttgcag ggtgttgaat aactatcctg gccgtatcct
    12361 gaaaccgctg atgtggttaa gcctgttttt tttatgtgtt ttcctgggag tatatctgaa
    12421 aaaaagttat caggcggaaa aagaggccct tcaaaaagag gtgggttacc tgtttatcaa
    12481 tgccgtaaaa aacatcgaag ggggcttgct gaacaggctg gtattccgtc ctgacagtac
    12541 ggaccatatc atcgtacgac atctattcga ttcaaacgat tctacccaga cgctggactt
    12601 tataggaaaa agctcgatgc ccctgccgga aggcaaaacc aggattaaaa tagcggtgag
    12661 caaggaaaaa caggtttccc tccaggagac cgaaggaatg atttcaatga ttgtgcagat
    12721 ggatcaggac agcctgtccg gggattctct ggtcaggata cgtcccatgg aggcctcctc
    12781 taagatagcc acggaactca gaaaccggtt tgaactcaat ctacaggccg ctggccttcc
    12841 ggtgaaatat acctttacac aattccggga caccggaaac ctgcaggatg ataaaaaaga
    12901 tattgccgga tcctattttg atattgcttc cggggaacgt tatgaagttg ccctgagcca
    12961 gtaccggggc ctggtcctca aaaaaatggc gcctgaaatc atcctggcag gtcttttact
    13021 cttatgcctg ggcctggctt tttacagtat ggcccggacc attaaacggg aaaagcaact
    13081 cctggctatt aaaagcgatt ttatccagaa catgacgcat gaactgaaaa cacctatttc
    13141 caccgtgggc gtcgccctcg aagcgcttca ggatttcgat gcgatccagg acagccggaa
    13201 aagggaggaa tacctgaata tttcgaaaaa cgaattgaac cgcctatccc tgttggtaga
    13261 ccgggtcctt tccgtgtccg gcatcgacaa agccctgccc cccgcacatc ctgagcggat
    13321 tgacctggcc gccctggtca acagcattgc cgactccttc cgcgtacaaa ctgaaaaaca
    13381 aaatattacc attgatgtcc atatcaagga tgatcccctg gtcctgcatg cagaccggca
    13441 atgggtggcc ggaattgttt ataacctcat ggacaatgcc attaaatacg ggaataagga
    13501 tggcggccgg gtggatgtat ttcttaccag ggaggccgga caaatcggga tccgggtaaa
    13561 agacaacggc cccgggatcg gaagagaata tcaaagccgg gtctttgacc gtttttaccg
    13621 cattccccag ggcaatgtac atacggtcaa gggccatgga cttggactgg cctatgtacg
    13681 ccgggtggta aaagaaatgg ggggaaaaat caacctggaa agcgaagcgg gcaagggtgc
    13741 ggtatttacg gtgagcctgc cctcctgaat aaaaaatcca tgattaagtt attatatgtc
    13801 gaagacgaac ccagcctggc caagattgta acggagaccc tgggaagccg gcagtttgaa
    13861 gtgcgccata tcggccgggg tgatctggct atggacgctt tccgggattt cagaccggat
    13921 atctgtgtat tggatgtgat gttgccctat gtggatggtt ttagcctggg aaaagagatc
    13981 agaagtctgg acagcaaagt ccccatcatt tttttgacgg caaaaagtca gacggaagac
    14041 gcattacagg gatttggttc cggaggcaac gattatatcc ggaaaccttt cagcatggaa
    14101 gaactcatcg tccggataaa aaacctgctg gctttgtcca accagtccag aaatttagag
    14161 gtcagtccgg aagccctgtc cctgggacgt tatcttttct atgcaggcag gcaggaactg
    14221 catttgggcg attccgtcaa aaggctgtcc caccgtgaaa cagagttgtt gtccatgctg
    14281 atccgccaaa aacaggaagc cgtccttcgc aaaaccatcc tggataagat ctggggtaac
    14341 gattcgtttt ttaattctcg caacctggat gtttatatca ccaaactcag ggattacctc
    14401 aaagaagatg aaaaaatcca gatcattacc ctgaaggggg tggggtatcg gttggtggat
    14461 taagattgag ggttgaggat tgaggattga ggagagggtt gaggatcagg ttgaggttaa
    14521 ggttgaggtt aagaatatga aaatgggttt tctgccggaa tgtgggtgac cttcatgccg
    14581 ggaatgttgt tgcggatcca ttgggccata taacgggatc ccggttcttc gctgggaata
    14641 tgaccaagca caatcaaaga aacggtctgt ccttttgccc gggcatccct tatgtattcg
    14701 gccgtttccc attcctgcaa ctcaccgcat atcgcaacat ccggttttgt ctttccgatc
    14761 cggtctatct gtctccttcc gcctgaagct ccgggcatca ggagaatctt cttacatgac
    14821 tgggtgacat ggccaatata tctgaggtgg ctgatgtcaa ggcctttttt gagctgtttg
    14881 atcaggcttt tcagattcat ggggttttcc aattcgatga ttccggattc ttcatcgtac
    14941 cggctggtcc aacccaggtc ttcaaggacc cccgccatga cgccgtcagg ttggtgacgg
    15001 tgaatataat cgtgattgcg ccagaccgca atcttgtgtt tttcgagcag ggcggattta
    15061 taacggaaaa cctcatcgtt ttcaagccaa tcggtctcgt ccaggtggtt gtagaaagaa
    15121 ggttcatgaa ctataataaa attggctttc agggcaatgg ttttttcaat gaccggaatg
    15181 gttggaaaca tggtggtgac gataccggta acgaccgtat cccggctgcc tgatttcagg
    15241 gtatccaccg tgatccgaaa tggagccccg gggacttccc ggataaaacg gtccatgatc
    15301 tggccgaccg tccaggactc ttgcccgggc ctccggagtc cggggaagaa aaaagaaccg
    15361 gcggcaaacc cggtcaactt aagaaaatgc cggcgcggat aaggctttac cgggaggatt
    15421 tgtttaaggg tcattttcat tttattttaa tgatctaaag ataaggctgc cttgagacca
    15481 cttgatttca ccgggccaaa ctatctatct ttaattaaaa ataggacatg atgtacaaga
    15541 taacccggtt tttggtatgg ggatttctcc ttcttttttc ctgtaaaacc gcctatgtcc
    15601 cgaatacggt aaacgttccc ctttttgacg atgccgggca aatgcggctg atggcggact
    15661 tcaacggaaa catccagttt gccgccagtc cgggaagaaa ttttggcatt atggccaatg
    15721 ccttgtttcg aaaggatggc gagaacacag atgatctgtt tggaaagggt tatctggtcg
    15781 aagcgggcct gggtggattc aaggcttcag ggcccctgcg atgggaaact tttgccggcc
    15841 ttggttttgg caagaccgaa acccgcgatt ttggcaaaaa atttgaagcc aagggcgccc
    15901 ggtttttttt ccagcccagc gtcggttttc atcatgatat ttttgaaatc gcttttaccc
    15961 caagattggt tgccggaaag tttcagaaac ccgaaaccac ctataccacc caggaattgt
    16021 tagataacag gctttatgaa atcgacgagc cggtctggtt ttttgccgaa cccgccatta
    16081 ccggcaggat cggttacaaa tggatcaaac ttcaggtcca ggtcggccgc tcttttaagc
    16141 tcaacgacaa gcccctttcc tttgaaaaag aaatgaacag catcggcctg atcctggatt
    16201 tcggcaggag gtagatttat ggaggggttt tccactcacc atccactact caccatccac
    16261 caaagaacaa gttgaggctt aatagtcctt attttttgaa tccagccaaa tcgtgaccgg
    16321 gccgtcgttt accaaatgta cctgcatcat ggccccgaat tttcccgttt cggtctgaat
    16381 cccggttgct ttcaaggcct caataaaccg gccgtataag ggagtggccg tctccgggcg
    16441 ggcagcccgg atataagaag gccggttgcc ttttttggta ttggcgtgca gggtaaactg
    16501 gctgacaagc agaatggacc ctcccgtttc caataccgat ttgttcatga tgcccgcttc
    16561 atcgtcaaag atcctcagct ggcttatttt gtggaccagg tattgaatat cttcctcccg
    16621 gtcttcattt tcaattccaa gaaagaccaa tattcccctt ccgatcctgc cgctcagggt
    16681 cccgtcttca aggaccactt ccgccttacc cactctttgt atcaaagccc tcatattcca
    16741 tataattcaa tgagaatcaa taattttatc aacattaatg aattaactac tatataaaat
    16801 acaaatcctc agaatgaatt taaaatactt atccacaata atttgtggcc cggtggattt
    16861 taaatgtcaa cagagcaaaa gatatccacc acggacttcc ttcatataat ccatacccat
    16921 atatgtgtga ataaggagcg catttcgaac aatgaacaca caggtccacg gattctttct
    16981 tttagctgtg gacagcgtga attaacaaaa tatataactt gttaatgaaa ccagtattta
    17041 tcacatttcc agtaaattag tgctaacatt attttccata tattttgtga ataatctaag
    17101 gcggattccg gagcctgccc ggtttatcca catatctaca tcactaataa taatagctta
    17161 attgtaattt aaattaatct ataaattatt attaccggct aaggactggc gttcgggaaa
    17221 ccgggtaccg gcctatattt gggccattcc tgaaaaaaat gaaacattct ctcatccctt
    17281 ttcccgtgaa agcctggttg tggaccggcc tggtcatgat tttttttcag atcctcatcg
    17341 ggggcatcac ccggctcacc ggcagcgggc tttcgatcac caaatgggaa atcgtgaccg
    17401 ggaccctgcc gcccctcaac cgggcggact gggaagaagc cttcagcttg tataaggaaa
    17461 cccctcagta tcagaaaatc aaccggggaa tgagcctggg agaattcaaa tggatctatt
    17521 tctgggaata tctccaccgt ttatgggcca ggctcatggg ttttgttttt ttactcccgt
    17581 ttgtattttt ctgggccaag ggatacctct atcctcaatt aaaaaaaggg ctggttaccg
    17641 tgtttatcct ggctggaata gtcggtgctt tcggatggat catggtggcc agcggcctga
    17701 tccaccggcc ctgggtcaac gcctacaaac tcaccctgca cctgggcctg gctttggtca
    17761 ccttatctta tctctggtgg gtttatctca aaaccaattc cccggcatct tatcccggcc
    17821 tgggggccag gcaaaatacc gctttgattt ttttggtact ggtttgcatt cagatcctcc
    17881 tgggaggaat gatgagtgga atgaaagcgg ccctggttta tcccactttt ccgaagatga
    17941 accaatattg gatcgatccc atccttttcc gaggggattc ctggaaatgg cagcatttta
    18001 tggattacga ttcctatccg tttttacccg ccctggtcca ggttttgcac cgcataacgg
    18061 ctctggtact cctggtttta ttttttaccc gcatgtaccg gttttttcat ctaaaactcg
    18121 ttcgctgggt ggccctccta ctagtcatcc aggttaccct gggcatcatt accctgcttc
    18181 gatccagggg attcataccg gtcaatctgg gtgtcctgca ccaagctgca gcgattgtat
    18241 tgctgctggc gactttgagg tggtggttta taagccgggc ggggtccggg aatttggaag
    18301 gggaacaacc gcaaggatcg ccgacccggg tttcaaataa atagaaaggt agtcaaaaaa
    18361 agggcaaact gttaaaaatt cctatattcg gtttcgaata aaaaaacctt aattcttcat
    18421 tcaaaaaatc atcttatgac ttcgactcaa attgaggaaa aaccggatat gggcttgttc
    18481 tggggttgtt tcattgccct gattgccacg gcttttggct ttatcctgag ggctttattg
    18541 ctcgaagacc ttggcaatga atttaatctg accaataccc agaaagggga gatctccgga
    18601 gtgggactct ggccctttgc catcagcatc gtattgttca gtttgtttat cgaccggatc
    18661 ggataccggg cagccatgat ctttgccttt gtggcccaga tcctgtccgc ggtgatcacc
    18721 attttctttg caaaagggta ttggggactg tggatcggca cctttatcct ggcgctcggc
    18781 aacggagcga tcgaggcttt tatcaatccg gtggtggcca ccatgttttc aaaaaacaaa
    18841 accaaatggt tcaacacact tcatgcaggc tggccgggcg gcatggtgat cggcggcatc
    18901 tttgccctga tgctgggccc cgcagtatcc tggagggtaa agatcggcct cattcttata
    18961 ccggtattat tgtatggttt cctgctttgg aataaaaaat tcccggttca cgagcgcgta
    19021 aaggccggag tgacctacaa tgaaatgctc agagaagtgg gcatgggagg cgcttttatc
    19081 atcctttggc tgatcttttc acagcttggc caggtgatcg gatggggcgg agtgaccagc
    19141 ctcatcatag cggttttaac caccctggct tttggatatt atatcaaatg ggccattggc
    19201 caacccttgt atatctttct cttgctgatc atgattccgc tggctactac cgaactgggt
    19261 acggacagct gggttaccga ccttatggga cccgccatgc ataatatcgg ggttcaagcc
    19321 gggtggatcc tggtttatac ttccctgatc atgtcggtcc ttcgtttttt ttccgggccc
    19381 attattcaca aactcaaccc cctgggcctt ctggcagtga gctgcgtatt ggctatcctg
    19441 ggccttttta tgctctctac gagcacgggc ataggcatat tgattgccgc gaccatttat
    19501 ggcgtgggga aaacttattt ctggcccacc atgctgggta tcggagctga acagtttcct
    19561 aaagggggcg ccctggccct gaacatcatc ggcggggtgg gcatgctggg tgtgggtatt
    19621 gtaggcacgg tgctcctggg caatgtgcag gaccggtctg tcgtaaatac cctgaaggat
    19681 atggacacca agaacaatac caccctgagt tcgacctatg ccaccgtgga aaaaggatcc
    19741 attttcggca aatacatggc cctggacggg gacaaagtcg ccgcagccgc cgagccggat
    19801 aaaacatccc tgacgaacgc cgcctcggaa gctaaaaaga gcgctttgcg taccgtggcg
    19861 cttttgccgg tatttaccct gctttgtttc ctggccctgg ttttctattt ccgcagcaaa
    19921 gggggatacc aggcggtgca tttggaataa gaaaattcat tttcgagtta taccacctgc
    19981 tcgaatgaga cctggaaggt ttctgttttt aacgtattaa accttccagg tcttgttcga
    20041 attttattaa tatcacgcca tgattgatgc gctccggaaa cagtttaatg aatcgtttac
    20101 tcaggaaaaa tatgaagcat ataaacaggc ggtagcggga gctgcggggg aagctcccct
    20161 gttcaggctg gctgaaaccc ctgttttcat accggcttat tttaaagaaa aagtattaag
    20221 ggcctgcgaa gacctgatcg ataccattac ccggccggat tttttatcgg cttccgaagg
    20281 cgccatcccc tcccatctgc aatttgccgg tgatccgggc tattgcccct tgctgatctt
    20341 tgatttcggc gtttgccggg atgagcaggg aaccctgtca ccccaactga tcgaaatgca
    20401 gggcttccct tccctttact gtttccagat tgcccaggcc gaagcctgca ggaaagtatt
    20461 tgaaattcct ccgggtcttg acaattatat tggagtgagc aaggaagaat atattcaatt
    20521 gctaaacaaa accatactgg acggagcgga ccctgccgaa accattttac tggattataa
    20581 accccaggac cagaaaacac gggttgattt tacggctacc aaaaaatacc tgggcataga
    20641 cgccgtctgt gtgaccgacc tcaaacagga agggaaaggc ttgtattacc tcaaagaagg
    20701 caaaaaacaa aagatttcac gcatatacaa ccgtttgatt tttgacgagt tctttgcaat
    20761 gaaaataccc tgcgtggacc ttaggcagga cctggaagtg agttgggtag accaccccaa
    20821 ctggttttgc cgtatatcca aatacagcct gccctttatc accagtgatt ttgtaccgga
    20881 cacccgtttt gtttccgatc ttaaaaacat tccggaggac cttcaaaatt atgtattaaa
    20941 acccctgtat tcctacgcgg gcatgggcgt ggtgatcgat gtgaccaaag aggatctcga
    21001 aaagatcaaa gatcctgaaa actggatcct ccagaagaaa gtgcattatc acccggccat
    21061 catcactccc gatattcccg ccaaatgcga gattcgcatg atgctgatct gggaatcccc
    21121 ggataaaaga ccccgcgtgg tgcacaacct ggccaggatc agcaaaggaa agatgatcgg
    21181 cgtgcggtac aacgccgatt tcagttgggt gggttcttcc acctgttttt ttgaataaaa
    21241 ccaatgcaaa ttccttcact ggtgacttcc gtgcccgatc tgcatactgc ccggatttta
    21301 gcggcggcag gggttcccta tatcgccttt catcagaaag tcaaaaacct ggaggagatc
    21361 aaaccctggc ttgaaggccc ccggaccggt atggaaatga aggacccgga tcaaagcctg
    21421 cctgcaactg attttctgat tatgccggca tcctggtacg atctgttttc gttttcggga
    21481 aggaccatat tctggaaaac cggtgatgag catttttatt ttggagatgt agtccttgag
    21541 aaaggaacgc tattaacacc aggtgacaat ctgcccggcc gccggtttta tcactattcg
    21601 gattacaggg gcccgggaga agaaaattca gatacctggt tggattacga agaagatttt
    21661 aacttgtttg aatccttgtt tttataaatt ctgttttcat gagtcaatga ggataaaggc
    21721 gggcggagta taaaagttac ttaccacatt caaggagaaa aaaggattaa aagccgtcag
    21781 gcggcggata cattccggaa cacgaagtac aagcccgtgt aacctttcga aaattccgcc
    21841 gttatatggt tggattcctt ttattcctag tctggttcac cccacacatt aaaaccaatt
    21901 ggtttaaaat gccactttcc ctttatttcc tcctcttatt tgcccggagc gctttgtcct
    21961 ttgacttgcc ggaaaatttt ttagtccccg gaaaaaccgt aaatgcccgg attgacccgc
    22021 aaaaaacgga tacttatttt gtcaggatgt tgccggggca gttctcaagc atcaggctta
    22081 cggaaaaaga ggtgcgcctt tatgccacgg tttacgatcc ccaggacagc ctgatacagc
    22141 tcatcgatga aaaccggatg ggcggcaagg aaatcatatc cattttttcc aaaaaaaaag
    22201 gagattataa aatcagagta ggctggaatt tttccaagcc ttattcaggt aattattcca
    22261 tcactctgga ccggctcgaa gccaccggaa aaacaccggc ccttaaagcc gggcagttga
    22321 tggagggatg gtatgaaaac caggagcccg gagcggcggt tgctgtcatc cgggatggac
    22381 aaccggtttt cgttgcgacc aggggcatgg ccaacctgga agaaaagatc cccatcacca
    22441 gccagtccgt gtttgaactg gcctcctgct ccaaacaatt taccggcctg gccgtagcca
    22501 ttctgattga tcaaaaaaaa ctttccctgg aagaggatat ccggacgtat ctcccggaaa
    22561 tgcccgatta cggagagaaa atcacggtcg gtcacctggt ctaccatact tcaggcattc
    22621 gcaataccga tgcgctggaa atggccgggt ttaccccgga agaccggatc accctgccaa
    22681 tgtgtgtaaa atttgccgcg aatcaaaaat ccctgaagtt taagcccggg gagcggttca
    22741 attattccaa tactaattac aatctcctgg cggaaatcgt tgcgcgcgtg accgggcaat
    22801 cgttttccgc atggaccagg gagcatatgt ttaaacccct gggtatgcac tccacgtttt
    22861 ttaaggaaaa cccaggcgaa atttacaaat ataaagtcct gtcttatcaa agcggccggg
    22921 agggattccg gcaaaggccc aataactggg ctgctgcagg gtcctcagct ctttgcacca
    22981 atatcgacga cctggtaaaa tgggtaaacg gtttcgacac caaatcattg atcacccccg
    23041 gggtggaaca attgctttat gcggaaggct ccctgcgcga agacggccgt acccggtatg
    23101 catttggcaa tgaattcaga actttcgacg gcattcggga aatcatgcat ctgggactgg
    23161 tgataggtta tcgaacggcc attgtgcgtt atcccgagcg gaaactggct tttattttcc
    23221 tgagcaatga cgacaacgac gccgcctatc agcgtattcc aaaaattcgg gacctgtttt
    23281 tgtttggtga cctgagggaa acaaaaccga acttaaaaaa cttcccgtcg gcggaaaccg
    23341 taatcaaaaa catagaagcg gatgaaaaat accaagaagc cattgaccta aaaccctacg
    23401 aaggcgttta ttgctctgaa gaagtgtccg ccgtatggaa tttaaaaaaa aaccaggaac
    23461 gtttggagat ccggcatccg cgaatggacc ctatgcttct caaaaacacg ggaccggatc
    23521 aattcggatt tattgaattc agtcgcaatg agtccggggt ggtaaaggga ctgaaaatcc
    23581 gggacgaagg gatcgagttt atgaaaatga agtgaaggaa tgcaacccgc tccaatattt
    23641 ttacaataac gaattgattt ttggcatcgg gctcatccat tcatcaaatc gtttactgcc
    23701 aatacgtgaa tttaccgaag gaattagggt atgatcgctg agagacgcac cgaaataagt
    23761 cgcgtttaaa tccaagacta tctgccgggg gtcatgattc agtaccagat accgtcggac
    23821 caaatcatcc aacctgaatt tttcaggccc tgcgagttct accgtcgtat tcaacggaac
    23881 tccggccatt aaatcagcca gggtatccac tacatcatcc gccgcgaccg gttggatcca
    23941 ggcggagggc aaataaaccg ttgatccgcg agtggcagat tgtgcaatca cggctataaa
    24001 ttcgaaaaac tgagttgaac ggacaatggt ataagggatc cccgattctt tgatcaggtt
    24061 ttcctgaacc cgcttcgccc ggaaatagcc gctttcctgg aggagatctg tgccgacaac
    24121 cgacaagaca agatgatgac ggattccggc cgcagcttcc gcttccagaa gatgttgtcc
    24181 agcagtttgg aaaaaatgca ttaccgcccg atcatccaaa gaagacgaat tggatacatc
    24241 cactacggca taagcccctt cgactgcttc cgccaaacct tggccggtaa aggcattaac
    24301 cccggaggat ggcgaagcgg ctatgacgtc atggccttcg gaacgcagct tggccgccag
    24361 cctggatcca ataaatcccg ttccgccgat gattactatt ttcataggat actaaaatta
    24421 gtaacgcatg caattttaaa ccggaaaaaa cggccattga agacctctga gatccactta
    24481 tcgttttttc cccacaaagc ggatcaccga accccgcccg ttgtgataga gcccttcgtg
    24541 gagttcaatt acttcttctc tcaattccag gatatcgtaa tgatcgaaat ccgctgaaat
    24601 ttcgtcaaca gaaaacaggg aatccaggtc ttttggcccg ccgacctttt catcagcggc
    24661 caggtattcc aggtggtttt tactgaaagc ttcgaaaatg accattcccc ctgagaccac
    24721 cagccgatcc aaagccttgt gcatccgaga tttgatctcc ggagggaaat gggcataaat
    24781 caaggccagg acatcaaagg atttgtccct gtaatccagg tcctgcaatt gaccgacccg
    24841 gtaatcgatc gataacttgt ttttagcggc caggatccgg gcctttctct ggccttcaac
    24901 gctgatgtca aaagcggaaa ccttccagcc gagccgtgct gcaaatacgg cgttgcggcc
    24961 ttcgccttcg gcgggaaaaa ggatatttcc ggggacgagc tttatcaact gttcttttaa
    25021 aaagtgattg gcttgctcac cataagcaaa ggcttccgtt tcgtatcttt tattccattt
    25081 atcggtccag gaatcggtca tggagtttga attttttcgg tatcgggcaa aatatggatg
    25141 actacatttc ctttttttcg gagcgtttcg gcataagcat gggcttccct gatttgctca
    25201 agggggtatc gccggtcaat gacggtcttt aaatctcccg actcaaacag ccctttaaga
    25261 acgtggagat ctccgggacg gaaacccggg gaacccttta ccgagacata ttttcctccc
    25321 ggttttaaca gatgtttacc ggcggatttg gacgttttac ccaccgcgtc aaaaacgatg
    25381 tcaaaacggg attccagctt cgtataatcc tgttttgtat aatcgatgac ccgatcggcg
    25441 ccaagcaatt ggaccaggtt catattagca gtgctgcata ccgccgtgac ccgggctcca
    25501 aaatatttgc ccaactggac cgcaaaggtc cccacgctgc ccgaggcccc gtaaatcaga
    25561 aggtcttgtc ctttttttat gccggccttt ttcaaaaaac ccaaagcagt cagtccgccg
    25621 gtcgggacgg tggccgcttc ctcaaagctc atgttggagg gttttaagga gatcacctgg
    25681 ttttcgggaa ggcatttgta ctctgcataa gccccgaagt taaacccgca aaacgcaaaa
    25741 acggcgtcgc cttttttaaa tgatttgact ttattgccgg tttcttcaac gactcccgcc
    25801 aggtcaagac ccaggacctt tacctttttt ggcctgaata gaccattgaa cagccttgcg
    25861 agaaaaggat ccgctttccg cagacgccag tccgccgccg ttaccgtagt ggcatatatt
    25921 tttatcaata tctcgttttc ttttggaacc ggcttttgta tatttttcat ttccagcact
    25981 tcgggcgttc cgtatcgttc ataaactact gctttcattt ttcttattgt ttaatttaat
    26041 cggatttggg ttaaagaata caaacaggct gtttcattcc attatctgag caccgtgacc
    26101 ggaccggctg caggcgcatt aaatcccctg gtcaacaacc ataccgccag gctcatttca
    26161 tagaggaaga ccgggatggc cagtaagcca gcccaatggg aaacctggga aaaagcgcca
    26221 aacatcacca ggatgccgca tccgaagatc aaaggccctc cggccagccc aagtatggat
    26281 ataaaccgag gcaccagcct ggaggaaaac aacaaataag cggtcatgag cgtgctgggc
    26341 cccaggagga tattgggccc aaacgtaaaa gtccagtcgt gcagcgcttt cagggatttt
    26401 ccggcgatca ggtaagaggc cgtattcggg gcggcctcct taataaattc atgattcagg
    26461 ctcacaatgg ccaggaggct caggatgccg accaggatca tggccgcttc cagcaggcgg
    26521 aaaccgacgc aggccagggc cattctgtcg ttgtactttt tcagtacggg atacaaagca
    26581 agcggggtgc cgatgacggc cagggcggta atgacttcaa aaaaggcgcc ccataaaacc
    26641 cgggcttcaa gagggcgggt ctgaataatg taatcgggat ttttgaggat ggggtcatat
    26701 aaaagggccc cggtgatggc ggcaaccgct gcgatgataa aaaatacacc ggcaaggttg
    26761 gcgctggttc tggttgtttt catttttaat atattttgca atggtttaat aagggttgag
    26821 gtgatgttat ttcgaaacat gcaggcggat caacaggctt ctgtggtatg attcccggtc
    26881 gtaggagaca ttggtcaaac cataacagta ccggatatcc agcacggccc ttttttgttt
    26941 gatcctgaaa gtcagtcccc ctcccgcctg aaggccggca tcgaggcgct tataagcgcc
    27001 ggcttctttt tcaaaagaca ggtctgtggt tgttccatcc agtttttgag tcccgtcgaa
    27061 attgtaaccc agggacggtc ccgcattcag gtgaaaccgg cccaaatgaa gtcgggcaag
    27121 caccggcaat tcaagggtat agaaccggag ggtggtttca tcctgggtga gcgggttgtt
    27181 gacctttaat tttccgcccc tcatgatgaa agacaattcg gggaccaggg ataaacggga
    27241 ggcaatgcct gcctgcaggg aaaatccgat ttgtacgcca tgggccggtt ttttaaaatc
    27301 tttcagggaa gggttggctt ttccataatt cagttcggtg ttcaccgcgc tgataaaaaa
    27361 gtccaaatgg gttttgggta ccgccggttt tgaggactgg aattggccaa acgaaaagga
    27421 aggcaaaaaa accagggcca ggatgaataa ggtttgtact gaatttttca taacttttgt
    27481 tttttagatt gtttaaataa ttaagtggaa caaaagtata agggggaaaa ggctgttaaa
    27541 accgggatat gccaggcggg caaagatgtc tgcaaaccgg ggttttgggt ctgcaaaagg
    27601 gcggagccga tttcgcgcta aggtgcaaag acgcgaaggg ggaaggtatt aagccagatt
    27661 aaacagcaag ttcaatgcct ttcgctcacc ataaccaata gtctgaactg aagggtttcg
    27721 cgcaaaggcg caaagacgcg aacgggaaag tattagatct tacggaagtc ttatggaagg
    27781 gtttcgcgtt gaggcgtaaa gtcgcgaaag agatattaga ggcggccgct caaataacta
    27841 tagccggctg ttgatgagct ggaagaagtc ctctttgtaa gtatcggaga tggggatcaa
    27901 ttcccccccg atttttatcc ttcccctttc tatggattct atctgatgca gagccaccat
    27961 ataggacttg tgcacccggc acacctgact gggagggatc agcttttcaa attcactgaa
    28021 attctgaagg gtcatgaccc gcttattcat cgtatggatc ctccggtaat ccctcatgcc
    28081 ttcgatgtag acgatatcgc tgatcatgat tttttcaagc cggttttccg ttttgacgaa
    28141 aataaaatcc ggggaaggtt cggtggggcg atggaccagg ttttcccggg ctttgtttac
    28201 ggcctgtaaa aaccggttga aggtaaaggg ttttaaaagg tagtcggtga tctgcaattc
    28261 gtaacctttt aaggcgtatt cctgataagc ggtggtcagg atcacctggc tggatatttt
    28321 tgaactctcc agcaattcta tgccggacaa ttcatccatg ttgatatcca gaaacaaaag
    28381 gtccactttg ttattattca gataggacag cccggtgagg gcattgtcaa aggttgcgct
    28441 taaggttaaa aaggggacct tgagcacaaa atcttttgtt ttttcgagcg ccaggggctc
    28501 gtcttctata atgatgcagg tataattatc catggggaat ggccaggtgc acgaggtaac
    28561 ggtcgttggt tttttgcatc tctaaagtat gttttccgcc ataaatcagg tttagccgtt
    28621 ttttgatcag ttcattgccc aggcccccgg cagtggcttg gcctgccggc ctggggtcaa
    28681 atttgttttc gcacaggaat tgtatatctt cttcccggat ccggatttgg acccggatgg
    28741 cgttttcgag ctttttattg ttggtgtgtt taaaggcgtt ttcaatgaaa ggaataaaaa
    28801 ccatgggggc aatgctttta ttacccgcag ggccttcaac cgtaaaatgg atataatttt
    28861 cgttggccgt cctgattttt tgaagggcga tgtatttggt gatgtattcc agctctttgg
    28921 ccagggggat tttatcgccc ttggtttcat acaggataaa ccgaaggatg tccgacaatt
    28981 tattcaggta ttccgaggcg gccaccgaat cttttaagat cagcgcgtcg atattattta
    29041 tggtgttgaa caggaggtgg gggtcgagct gggacttgac caaggccatt tccatggcat
    29101 ggcttttatc ccgcaaagcc tctttaagct tgatctcatt ataccaggtg acaaagcccc
    29161 ggatgacgaa ggccacggtg ccgcaaagag cgccaatcgt ggtcatgacc aggatgaccc
    29221 ggccggcagt ggacctgccg tgtatccccc cttcatccat atctattatc cggcccgttt
    29281 caataaaata acggattata atataaccca ggatggcggc tacgcccgag gaaaggagcc
    29341 ccaaaagaat agcaggcaaa attttctttt gctccagata ttttggaaaa agtataaaat
    29401 aatagataaa atatgaaatg accgatggaa taaaggcaaa aaggagaaca ctgtaaaagg
    29461 cattccatac cctggcgacc tggtccgcct tatctgctac gctccgggaa taaattccca
    29521 gcacaataaa catcatcagg aaataacagg cccaaaaacc gatgtgcagc aggacgacga
    29581 aagatttttt catggactaa agccaaaggt caccttttgt tttggcatgg aaagaacgat
    29641 ctgcaaacgg ggcggatttg tctgcaaaat gggcgaaggg gcctctttta ccaatcaatt
    29701 tttttcaaat cgtatgtttt tgatccgccc gccggataat ctcaaaccgg tgaccttgcc
    29761 tttgatccgg tcaaattcaa tacgccccaa gtcgcagttg aataaccctg gggtcagtgg
    29821 atccggggtc aggtccaact cacccagcct gaaatgatgt aagcacaacc cctgagaaca
    29881 ggtattcaat gattcaccgc tacttcacca ccgccaaagc catgccgtcg tgttcctttg
    29941 cgcctatggt ctggaacaag gtcgccgtta cccggtggtc ccgggcgagg gctttgttga
    30001 accgctggac gccctgcacc aattcatcct cacagcgggg atccaggacc ttcccttccc
    30061 ggatgacgtt gtcacaaatg attaaagtcc cgggcctgga aagtttcacg gcgtaatcaa
    30121 aatattctgc ataaggcggt ttgtcggcgt caataaaaat aaggtcaaaa ggcccttcgt
    30181 ttgatgcgag gatgccgggc agcaaatcga gggctttgcc tacgcggatt tcaactttag
    30241 cgcccagccc ggccttgttg atattgcccc gggccacttc ggcgcagcgc ggatccagtt
    30301 ccagcgaaat cagccggccc ttttccgtca gggccctggc caagcatatc gtactatacc
    30361 ctcccagagt gcccaattcc aggatccgcc tggctccggt cagcaggacc ataagctgaa
    30421 gaaacctgcc ctgaaccggc gagacgctga tggccggcaa tccggcattt tcaatggatt
    30481 tcagcgtatc gtccaatacc ggatccgggg gcaataacag atcgctgatg tattgatcta
    30541 cctggccgaa gatttgtttg tccatacact cagggtctta atgaagaaag gcaaaaggat
    30601 caaagtgaaa attaagcaaa caaagaggaa ggcgccctcc tttttaattc cttaattctt
    30661 aattccttaa ttcttaattc ttaatggcca tcaccaccac ctccttacca tcttagccca
    30721 cctcgggtcg gtcactgccg tttgtgtttt tgacggctgg gtttggtggt cttcttcctt
    30781 tttacggtcg acattcacgg cgcgcccgat ggcatggggc ccccagcgtt tgcggatatg
    30841 gtcgagttcg ttgagcaggt tcatatcctc ttcggtatcg tcaaacaggt tgatctggta
    30901 atggccgtgg accaggccgc tgaatttgac cccgatcagg cggatcatct ggcgccgctc
    30961 ataaagttta tcaaagaggt catagacgtg gcgcagcagg atgcggtcgt tggcggtata
    31021 ggggatgcgg agctggcggg tataggtatt aaaatcggcg taacggatct tgacagtgac
    31081 cacagaacag agttttttgg actgcctgag gtcaaagccc agcttttcga ccatgctgag
    31141 caggatgttg cggatacggc ggaggtcgag ggtgtcctgg ctgaaggttt gttcgtggga
    31201 tatggatttg gcttcgctgt agggctggac cggggtgggg tcaaaagcat tggccctttc
    31261 ccagaggacc cgtccggttt ttccaaattc cctttccagc aggcggggcg ggatctcgct
    31321 cagtacccgc agggtgcgca cacccatgaa gctgagtttt ttatacatgg cctcgccgat
    31381 tcccggcatt ttggaagtgg acaagggggc caggaagtcc ttttccgttc ctttcaggat
    31441 ttctttggag ccgttgggct tggcttctcc ggtcccgatt ttggatacgg ttttatttac
    31501 cgaaagggcc atggaaatag gcaggccgat ttcgcggatc agacgtttgc gcaaatccac
    31561 cccccactgg taacagccaa aatagcggtc catcccgctg aggtccaggt aaaactcatc
    31621 aatggaagct ttttcaaaac aaggggcctc ccccgaaatc aattcggtca cctggcggga
    31681 atattcgctg taggcatcca tatccccgcg taggatgagg gcgtcggggc aaagccggcg
    31741 ggccattttc atgggcatgg ccgaatggat gccgaagcgg cgcgcctcgt aactgcagct
    31801 ggccaccaca ccccggtcgc cgctgccgcc gatcaggagg ggcttgccct tcagggaggc
    31861 gttgcgcagg cattccacag agacaaagaa ggaatcgagg tccatatgga ggatggcgcg
    31921 gtcgtacatg gtcggataat aaggcaaaga taggtcgaaa aaggtgacat ggtgagaaaa
    31981 gaggcctata tttaatcagg aaaacaagct aaaaacgaag tcgggtatat tcgaccgtcg
    32041 tgtgcccaat gacaacaaac ataaaaccag acgccgagat attgagggtt catttgggaa
    32101 agtttaaatt tggactcaaa gaggatgggg acctcatgat tgaccaaata agagcaatag
    32161 acaatcagcg actggtaaaa aaactgggag cagcggacgg agccacggca gaaaagatca
    32221 gagaaaactt aaaaatactt ttggacttag aataaccgta cgtataggtt gtatatgaac
    32281 gaagaactat ccaaagtgac catacacctg gtttcgagcc ttgacggttt tattgccaaa
    32341 aaagacaaca gcgtgtcctg gtttgaaacc acggattact acgaaaaggg agtcaccggg
    32401 gaagacgagg aggagttcct gaaaacgata ggctgttatg tgatcggcgc gcatacctat
    32461 gaacacaccc tggaaatagg atggccctac ggggagacgc cggtgacggt agtgacccac
    32521 cggaacctcc ggcacaatgg aaagaacatc gaattttatt caggaagcct ggaaaagctg
    32581 gtaaacgccc atttaaaacc caaatacagg aatatctgga ttgccggcgg tgccatgctt
    32641 gccgaagaca ttatccggct gaacctcgcc gatgatctga gattatctat cctccccgtc
    32701 atgctgggcg acggaaaacg ttttttggac cacatcggcc gggaggaggc attgcacctg
    32761 aaagacgtaa cggcttacaa aagcggaatg gtggaattgt ggtatgaaat aagataaaga
    32821 tcagttggaa caccgggaga ctttgctggc cttgaaggtc ggcgccgggg aggcttcata
    32881 gaccaggcag agaatcgcgc aatgctcctg agtggccgtg agattgaagc tgacgaattc
    32941 accggcgttc agggcctgga gatccttgct gttttgatcg tctccgcaat aattaagcac
    33001 ggagatgaca ttggaatact cctttttggt aaagatatcg gtccattttt cctgggcatc
    33061 gggcgaatac tcaatgagcc ggaaaacata ggcctcacag cccttacgga tcagttcggc
    33121 ctgtacccgg ccctccccgg gacaatctaa aacaggatcc gccccgggtt tgctgcaggc
    33181 aagaagaaaa aaacccccca aaagggaaaa aaataaaaac ctcatataaa taagaacgga
    33241 aaacggaaga ataaagttgg tcatttattt cacccgtatg cccaggccaa tggaatgcat
    33301 ggaacctgca aggtgataat tggccaggcc ataatcaaaa ctcagccggg aaagtttaag
    33361 gcccaggccg cccgagaaac ccgaaaggcc ccggtaattc tgatccttca gttcctggtg
    33421 acgctgatga tggtaagcca ggcgaagacg caggggcgcg tttttcccga tcagcacttc
    33481 cgaactgaaa atcaaatgcc tggccaggtt gtcgacaaaa cgttcgaccg ccgaaggcct
    33541 tccgacgggc tctccaaaaa ggttggtttc ctcatccggg gaatcgtacc gaaggtccca
    33601 gcggtggaga tcatgggcgg tgatggaaaa aaggatgggc acgtgttcca gtcgctgggt
    33661 aaagcccatt tcaatattaa aaggaagatt ttccctcgtt tcccaataag gatcccactg
    33721 cacccccata tgccggaaac tcagtccgaa agactgcctg ccggatttaa gcgcatacaa
    33781 caaacccagg tcgagaccca gtccgctgct gttatacgtt tccagttggg aaaaaatgaa
    33841 tttgaggttc aggccgatgc tcagcgattc aaacaatttt ttggatgccc cggctacaat
    33901 cgccatatcg cgggctttga attcattggt tttattgcca aattcatcgg cgccctgaaa
    33961 ccgcccatag tttaaatact ggagggcgcc gtggatggtc agattttctt tggaaaggtg
    34021 aaaagccgaa gcaagattgg aataggccgt tcctcccaga taaaaactct ggtttacggc
    34081 aatcgtgccc gtactttgga aagataaaac agccggattc atctgggcca gtaccacatc
    34141 gctgtcccgg gcgctaatat aacggccgcc cagtgccgta acgcgggcgg agggccccca
    34201 ttgtaaaaat tcatatatag cgcgacctcc gttttgcgca aaaatggaaa gcggcccaaa
    34261 actaaaaaac aggaagatca gaaagcggtg gcctgacttc atacgctgcc aaaaataagg
    34321 ggaataggat tagtaggatt tataggattg gtaggattta taggattagt aggattggta
    34381 ggattggtag gattggtaag atttttagta cttaaggatt aagaggagac cgtccatcct
    34441 aaaaatccta aaaatcctat caatcctatc catatcttca caggcaattc atagtcatga
    34501 aaagagttac cggcctgggt ggattttttt tcaaaaccca agatgccact tcacttaaat
    34561 cctggtattc gaaacatctg ggattggtca ccgatgaata cggagccacc ttttcctggc
    34621 acaaggaaaa cggacagaag gggtacaccg tgtggagttt atttaaagag gatacgacct
    34681 attttaatcc ggggaccaaa ccctttatga ttaatttcag ggtggaaaac ctggaggaac
    34741 tgttaaaaac actgaaagaa gagggggtac aggtagtggg tgatatggaa gtctatgaat
    34801 acggaaaatt cggatggatc ctggatcccg aaggaaacaa aattgagctc tgggagcccc
    34861 acgataacga atttggaaaa atacttcctt ctgaaaaccc cagcacctga tccaaacctc
    34921 atcccaaagt cccaaatcca cccatcctaa aatcccacaa acctaaaatc ctaaaatcct
    34981 acaaatccta tcaatcctat catgaaaaac gccatatcct ggttcgaaat cccgaccaca
    35041 gaccttgacc gggccaccgc cttttatgaa actatttttc aggtcaaact caaccccctt
    35101 gatttagcca atattaaaat gaggatgttt ccgctggagg atattatggg tgtgggcgga
    35161 gccttgactt atgcggaggg tttttataaa ccgtcagcca cggacggccc cctgatctat
    35221 ctcaacgcca atccggatgt acagattgtc ctggaccgca tacccgaagc gggaggaacg
    35281 atcctggtcc ccaaaaccga aatatctccg gagtatggat ttatggcggt attcctggat
    35341 tcagaaggca atcgcattgc gctgcattct gtacccgcta tccccggagc ttagcgaggg
    35401 gtagtgcaca cggaggccac ggcgctcacg gaggaatagt catttagaat tttttcataa
    35461 aaccactccg tgctctccaa tactccgtgc aaacttaagg gataggacgc cttcggcgcc
    35521 tatcaggctt aatgcgcgcg gaggccacgg ggtccacgga ggaatagtca tttagaattt
    35581 tttcataaaa ccactccgtg ctctccaata ctccgtgcga acccaaggga ggggcgcctt
    35641 cggcgcctag caggttcaat gcgcgcggag gccacggggt ccacggagga atattcattt
    35701 agaatttttt cataaaacca ctccgtgctc tccaatactc cgtgcgaacc caagggaggg
    35761 gcgccttcgg cgcctagcag gttcaatgcg cgcggaggcc acggggtcca cggaggaata
    35821 ttcatttaga attttttcat aaaaccactc cgtgctctcc aatactccgt gcgaacccaa
    35881 ggaaggggcg ccttcggcgc ctatcaggtt caatgcgcgc ggcgaccacg aggcccacgg
    35941 agaaataatt atttagaatt tttcccataa aaccactccg tgcgctccca ccctccgtgc
    36001 gaacccaagg agaatcatac tctcccccaa gcaaacatct ctttatcttg ttagtacaat
    36061 gcgcctttat aaacagattg caagcattct attgctgaca gccctcctcc tcgaactgag
    36121 agctcaggaa acttattatc ccgattccac ctgggcccgg aaaaagcccc aggagttgaa
    36181 catgaacaac gaaggcattg aagaagctgt tcgttttgcc atggccaatg aaacccacac
    36241 cgataaaaat ttaagcgcag cgatgtataa agcgtatgag agggaaccgg gatattccat
    36301 tctgggtccg gtccggccga ggggaggccc tgccggtctg atactgaaaa atgggtatat
    36361 cgttgccgaa tggggagatc ttcaaagggt ggacatgacc ttcagtgtta cgaagagtta
    36421 cctttccacc ctggcaggcc tcgcggcaga cagccgcctg atccggagca ttgacgaccc
    36481 ggtacgtgga tatgtatggg atgaaaagtt tgaaggcccg cataattcaa aaatcacctg
    36541 gaggcatttg ctgaatcaaa gctcagactg gtcgggctgt ttatttgaca tctgtgattg
    36601 ggcagaccgc cccccttctt ccggtacccc ggatgaatgg aaaagccgga aactgctgga
    36661 acccggttct caatttgaat acaacgatgt ccgggtcaat ttgctggcct atgccctgct
    36721 tcaggtctgg cgaaaaccgc ttccggtatt attaaaggaa aaaataatgg atcccatcgg
    36781 ggcctcctcc acctggcgtt ggtatggata tgaaaattca tttgtaaatg tggatggatt
    36841 aatggtccaa tcggtgagcg ggggcgggca tttcggcggt ggcttgttta tcaacacgct
    36901 tgatcaggcg agattcggcc tcttgttttt aagaaaaggc aaatggaaaa accaacaact
    36961 gttttcggaa aagtggataa atgcggtccg gcagccgtct gccccgaatc catcttatgg
    37021 actgatgtgg tggaccaatg aaaaaaatga actggcggga gtttcaaaga atatttatta
    37081 tgccaatgga tttggcggga attatatcgt gatcgatccg gagcacgacc tggtaatcgt
    37141 cacccggtgg ctggataccg ggaaactggg tgagttggta aaaagagtaa ttgatgcggt
    37201 ggaatcaaag tgaaagggtg actcaaaggc actccttcac tttaactcaa cctttcattt
    37261 tgacaagacc tcaagtacct tcgccggagt aaaaggcatg tggcgcagcg cctttccggt
    37321 gagtttcagg aatgcgttgg ttatagctcc gcccatcagc ggcaggctca actcacccag
    37381 gctggtaggg ggtaatgaag aaggcatcag gctgatttca atgctttccg ggcaatcggc
    37441 catacgaagg atgggataat cgtgaaaatt ggattgctgg acctggccgt ttttaaaggt
    37501 gatgctttcc tgcaatacgc tgctgatgcc catgacgatt cctccctcca tttgggcaat
    37561 ggcattatcc ggttgaacca cgaccccggc gtccaccgcg gcccatactt tgtgaacttt
    37621 tatttgcccg ctttcctggt ccagagatat ttccacgacc ccggaggtat aagaacctcc
    37681 atagtccgaa aaagccatgc cgtatgcgcg tcccggagca ggggatttgc cccatcctga
    37741 aagggcagcc gctttatcca gaagggcgag gtgcctgggc agatctttca tcatccggcg
    37801 gcggtattca tacggatcaa tctgaaggtc atgggcgatt tcatcgataa aagcttcctg
    37861 ggcaaattta ttggggccat ggcccacgga acgccaaaaa gcagtgcgta ccccgggatg
    37921 gcttacgcgg tgttcggtct gttggttagg tatggtataa taattcattc gaaccccacc
    37981 ggtctgcaaa cggttgtccg gaccggcaat ccggtgttgc catcctgtta aatggccatc
    38041 ccgatcaacg cctgcctcca tgcgttgcag gctcattggt cggaatgcgc catattgaat
    38101 atcgtcctcc cgggtccata tcagtttgag gggacggcgg acctgtttgg ccagcatggc
    38161 ggcttccagt gaaaaatctt cctgtctcct gccaaaccca ccaccgctgt aacaattgtg
    38221 gaaggtaacc ttggattcgt ccaggcctaa gactttggcg atcgcatttc tttcagtatc
    38281 cggcgcctgg gtgccggccc aggcttcggc gctttttccg tcggcagcga tatgaatcac
    38341 tgcgttcagg ggctccatct gggcatgata caaataatcg gtacggtatt cccgttcgta
    38401 tattttgtaa gcggattgaa tattcgaagg agcatcgccg gtgtccacca ccatcctgcc
    38461 ttttttattt tcgtcccgga tcaaaagctc aagggattta tagtcttcaa cgctgtcata
    38521 tccttttgcc cttgcctccc cggaccagcg gatctgcatt cgtttttttg ccttaagggc
    38581 ctgttcaaag gtattggcca cgactccgat cccgtggtcc attttgacaa gatcgaccag
    38641 cccatcagaa gtgcggacct cattttcatt cagcagttcc ggtgtggatc catataccgg
    38701 ggcacgggcg atggcggcat aaaccatgtc cggcaggctg aaatcaattc cgaatttggc
    38761 ccggccagtg gtcttttcag ggatatcgta acgcgggatg atctggccga tcaaccggaa
    38821 ttgttccggt tttttcagat cctgttcgtt tatttcagga acggatgggg gaatttgcgc
    38881 aaaagaggcg atttccccat aactcagttt tcgccccccg gcagggtcaa tgacatatcc
    38941 ccggtccgtt tttaatccgg attggggcaa tccccatttt ttcgccgcat tgtataaaag
    39001 gacatacctt gctttagcac cggcctggcg caaaggtttg taatatccgc ggacggtgcg
    39061 gctcccggcc gtgatcatgg gccctcccgg ggtcccgttc cattgcatgc cgtaaatacc
    39121 cggctcgacc ggggaatatt cgatggtcac tttgtcccaa tccgcatcca tttcttccgc
    39181 aaggattgca gccagggcag tcatagaacc ctgccccatt tcagcggccg gattataaat
    39241 cgtgatcttt ccgtcggcgg tgatttttac ccaggcggtg agctgctggt ctccactgga
    39301 agcggccggt tcagggtcac ctaaaaatct aggagctatt gctcctgccg ttaccgcaaa
    39361 actgaatccg cctgccgtca gcataaactg acgacggctc atgggatggg cccgtccttc
    39421 tttcttgttg ttcattgttt gccggattta gaaacttttt caatagcctt gagaatacgg
    39481 gtatacgttc cgcaacggca aagcaccccg ttcatgtatg aaatgatcag ttcccggtcg
    39541 ggatggggat tggctttgag aagggctgcg gcctgcatga tctggccgga ctggcaatac
    39601 ccgcattgag gcacctgttc ttcaatccat gctttctgca ggggatgcag ttcttctccc
    39661 gccaggcctt caatagtggt caccgacctc ccggcaacgg aagaaaccgg ggtcatgcat
    39721 gaccttactg caatcccgtc aaggtgcacc gtgcaggccc cacacagcga agcaccacaa
    39781 ccgtatttgg tcccggtcag ttgcagggca tccctcaggg tccaaagcaa ggtggcgtcg
    39841 ggggcggttt ccacctgtac cattttgcca ttgagtttaa aactggtttt cataataata
    39901 aacttaagaa aagattgacc gaaagatggt ttaggatatt caatttaaat aagaaatgcc
    39961 agaaatgccg ggaaaagatt agcttcaaaa cagataaagc cttattgttt acgccttaat
    40021 tgacggatgg cagccagatg ggaaaacagt accaggggga ccaccacggc cggcagccat
    40081 aaaaaaggga aatacaagag agctatattg ggttggtcga aagccaacaa ctgaaaagga
    40141 taggggaatg aaaataaagc gtggatgacg atgttggcca atagggcaag gcatatataa
    40201 ttccaggcca ggatgaattt aatgtccaaa ctttttttaa taaagccaaa ataatataca
    40261 ggcaaagcgg aaatgccgga aaggacatcg aagttccggc cctcaaaagt catcaattgg
    40321 ggaatggtac caaaagaaaa cagccagaaa agcacaagtt caaccgggat acggaccata
    40381 tgcaccagga tcagcgcttt cagatccagc ccatcgataa acaaacgacc ctttggagaa
    40441 acaaaaagaa caacaataaa aaccagcgga ggcagaatgc ccaataaaag ccggggagga
    40501 aaagcatcgg tgagggtata aaatccagtg gaagcgataa aaccctggag gcttaaccag
    40561 gccaacaaaa taaacactac gatccgggaa ttaaaggccg ctctgaaaaa caggtagagg
    40621 gtaagaaggg cggtcagtat gaaaaaggcg cttacgaatt ccggtacttg gtccattcga
    40681 ttcagacttc tgttatttta cctaatgtta ttaaatttcg aatgaaaatt tatgaaaaat
    40741 ccctcaaccc tcaaccctca accctcaaca aatgatccct acttttaatc cgggtatccg
    40801 ctttgcattg cccgccgatg ccgcatccat tgtagccctg ctcaacagcg cctaccgcgg
    40861 agaaggttcc aaacagggat ggacgacgga agcccatctg attgccgggg accgcaggac
    40921 ggatattcct cacctgatcg aaataatggc caggccggat tccgtttttt tagtttatca
    40981 ggacggcgac ggtcccatca cggcttgtgt aaatcttcaa cggcaaggaa acaagttata
    41041 ccttggcatg ttcagcgttt ctcctcagat gcagggaaaa ggaataggaa aacaattgtt
    41101 gaaagcggcc gatgaatatg caaggcacct gggatgcact gcgatttaca tgagcgtgat
    41161 cagcgtccgg gatgaattga tccggtggta tcagcgacat ggctataagg atacggggga
    41221 aagaaaacct tttgttgaag acggcctgac cggtaaacac cttaggcccc tggaatttat
    41281 gactttggag aaagaggtgt tatcgagtcc ggccccttcc taaaaacaat accccggcct
    41341 ttttaagggc aaaagtatta gcttaagtaa aggccgaagc ttagcttcgg ccaaacagtg
    41401 attagtttcg gccaaacagg gtgtgcatgg ccccggcttt ataaattcaa tatcgtattg
    41461 acaatgactc cgttgttgta ttgaaaaacg ttacggccgt tgatttcccg gccaagctgc
    41521 agcagttgct gtacaaatcc gccctccaga cggaaagttt tattgaaccg gtaccccagg
    41581 agggcgccga tccggttttg atcatagacg ttttcattta cattttttcc aaaaccgagc
    41641 aggatttcgt catagagggc gagataaggc gtatgatccg tcatcgcttt tttgccgagg
    41701 gcagtctgga tgcggaacat ataacggaat cggtgggtaa atacatattc atccgcatat
    41761 ttaaaggagg ggttggtaaa ccgcccggtc catctttgct ccaggataaa acggtggttc
    41821 atttcaaacc gcccgttttg gtcgttcagg ctggccagtt gatagatacg gtgttccgga
    41881 aaagttttgc ccgcggcctg gatggggtaa tctccataat taaacgtttc gataaaacca
    41941 taaccggcac gcagggtcag ctttggattg atcgtataat tcatgccaaa gcggtaaacg
    42001 ccctgttgcc aggtttttat cagttggtcc cgacgccatt ggaattccgc atggccggaa
    42061 aaccgttttg taaaagccac agtggtggtg ttggtgtacc aacctatggt attgggatcg
    42121 ctgatcctgg tattttggcc cagggccgga gtagaatacc cggcaagcag gaggagcaac
    42181 cccgccagtt tgttcacact tcttttcatc cggtcgctta ttttggtcgg aaataagtgg
    42241 ggcaaaagta atggaaaaat aatccaggaa tgaaaggcaa acggcaatat tccgttcaat
    42301 tgaaaaacag ggccggagtt agcatacagc aatccattat ctttgaccgc cctgaaatat
    42361 gaaaacaata aaattggcca tctggattgt ggcgatgcat ggatgggtga taacccaggc
    42421 gcaaaacgcc ttcgatcgcg acgataccct caggggctcc attacccgcg ccagggcttg
    42481 gtgggacctg aagtattacc gcttggaaat tgaaccaaaa ccggagtcaa aaaccctttc
    42541 gggaaaagtg gacattcatt atgaagtact gtttcccggc aaccttcttc agatcgatct
    42601 gcagccaccc ctggcaatag aaaaaatcat tcaaaacggg gaaaacctgg actttatcaa
    42661 agacggggaa aatgcccacc tcgtcacttt aaagaaaaac caggtccggg gcagcaggca
    42721 ggtccttgaa gtgtattatt ccggcgctcc aaaagaagcc aaaaacccgc cctggcaggg
    42781 aggggtgcaa tggacgcggg accaggatgg aaaccacttc atcgcaacat cctgccaggg
    42841 acttggcgca agcgcctggt ggccctgtaa agaccatatg tatgacaaac cggacagcgt
    42901 atctatttct gtcaccaccc ctccgggcct tatggacgtc tccaatggca aactccgctc
    42961 ggtcaccgct catccggatg gacggcggac ctttgaatgg tttgtgagca atcccatcaa
    43021 caattatggg gtaaatatga atatcggccg ttacgttcat ttttcggata cgtttcacgg
    43081 cgaacaggga atcctggacc tggattttta cgtccttccc tataatcttg aaaaagcaaa
    43141 agaacagttc aaacaggcca aactgatgct cagggccttt gaacactggt ttggccccta
    43201 tccgttttac cgggacggat ataaactggt ggaaacccca tacctgggga tggagcacca
    43261 aagttccatt acctacggca acggctttgc aaacggatac cggggcaccg acctctccgg
    43321 aaccggctgg gggctgaaat gggactttat catcatccac gaatccgggc acgaatggtt
    43381 tgccaacagc attacgtaca aagacatcgc cgatatgtgg gtgcatgaga gttttaccaa
    43441 ctactcggaa aatctgttta ccgaatatta ttatggaaaa gaagcgggat cggattatgt
    43501 gatcgggacc cggaaaaaaa tacggaatga caaacccatc atcggaacgt ataatgtaaa
    43561 ccggagcgga tcgggcgata tgtattacaa ggggggcaat atgctgcata cgatccggca
    43621 gatggtgaat aacgatgaaa aatggagaag gatcctgcgg aaaatgaacc agcagtttta
    43681 ccataaaacg gttgacggga gcgaaataga aaaattcatg agcaaggaaa ccggcaagga
    43741 cctctccaaa atttttgatc aatacctgcg ggatacccgc atccccacac tggagtatac
    43801 tttggaaaat aacaaactga cttaccggtg gaccaatgcc gtaccgggat tcaacatgcc
    43861 ggtccgcatc catctagtgc cgtaacaact aatatttgct aataattatt aaagattgta
    43921 atttgag
//
LOCUS       DAOQXT010000031        42920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1001_length_42920_cov_6.627832, whole genome shotgun sequence.
ACCESSION   DAOQXT010000031 DAOQXT010000000
VERSION     DAOQXT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 42920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42920
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1001_length_42920_cov_6.627832"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..618
                     /locus_tag="PKM27_07375"
     CDS             376..618
                     /locus_tag="PKM27_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HNR07119.1"
                     /translation="MARNTSILLGDHFEEFISSEIASGKYSSASEVVRTALRLLEQEE
                     EKRKMLVQALEQGEKSGFIKNFDPKAHLLKFHGKVK"
     gene            1099..2442
                     /locus_tag="PKM27_07380"
     CDS             1099..2442
                     /locus_tag="PKM27_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR07120.1"
                     /translation="METINPAPPSLRYAWMVVAILMLANVSSFIDRQILSLLVRPIKR
                     DMGLSDFEVSLLMGLSFALFYTFFGMVIGRLADQYNRRNIIVAGIATWSLMTALCGSV
                     RTYGQFFLARMGVGVGEATLSPSAYSIISDYFPKNKLASALSLYSLGIFLGSGIAMLI
                     GAGVIARLPTEGMVRIPLFGEVFPWQLLFVYIGLPGLLIALLILRVKEPVRKGLLLDQ
                     GQARNFNTREALKTILNKKAAFWGVCLGASFSALCSYGLNAWVPTYLQRTFDWSIPKS
                     GLYYGGVVVVSSLLGVLWGGWYADKLIHKSIPTGRIRVGIISTAGVLLSFPIPLIPDG
                     HLAVLAFAIPGFFIASNIGISAAAIQEIMPNQVRAFASSIFLFVLNLIALAMGPSLVA
                     AFTDFVFNDEGMLKYSLVFLLILGGSLGVGGFLWSIKNYKEELILHHAHLNSGKP"
     gene            2439..4247
                     /locus_tag="PKM27_07385"
     CDS             2439..4247
                     /locus_tag="PKM27_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNR07121.1"
                     /translation="MKKGELFSRRNLDFLLFETHKISELLGHSPYQAHDRETIGLALD
                     TAADIAERHMKPAFVDSDRNPPFIRDGQVIVHPVTHAFYHSFSESGLLASTFPESLGG
                     MQLPRSVFAAIDFIIGSAHNGFQMYTSLAVSASRLIESFGSQDQKDLYLPRLLSGDWA
                     ATMCLTESQAGSSLSDITTTAFLQPDGFYKIKGQKIFISAGDHDITENILHLVLARIE
                     GAPKGVKGLSLFTVPKKWLGDSGELTDNDITSVGIFHKMGQQSTPALHLEFGAGNHCR
                     GQLIGEPNQGLKYMFQMMNSSRLAVGLAGGYIASAAYYTSLQYARERIQGRNLEEKNP
                     LSEPVPIISHPDVRRLLFKQKAFTEACLSLVIQCQLYLDRLQTGVPGEEKKKYEQLLE
                     LMTPMVKVYGSEEGNDSVHAGLQVLGGYGYTRDFILEQLARDVRILSIYEGTTAIQGQ
                     SLLGRQILSGDGSVLDLWQMETGQDLGMASAHPDLKPYVLNLSENITGFISVSRELIR
                     KARETSAVPALADASLFVEWFGLLNAGWQWIKQAYTALVALEKAVSPEDRIFYRSKLE
                     TMKYFFHYELPRTQALLQRIGEAAPITLFPPDEILI"
     gene            4247..5032
                     /locus_tag="PKM27_07390"
     CDS             4247..5032
                     /locus_tag="PKM27_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNR07122.1"
                     /translation="MTEDKSYRKILVAREGPVLTIKINRPEKRNAIDDELLLEIEQAF
                     LQTDPSVKCILLCGEGDHFSAGLDLSALEEKDAVAGLRHSLMWHRVMDHIRFGLAPVI
                     AVLRGACVGGGLELAAACHIRVAEESAFYALPEGQRGIFMGGGGSVHLPGLIGLAVMT
                     DMMLTGRVIPAAEGSRLGFSQYLVENGKGMDHARGLAEKISANSSLTNYALTHVLPRI
                     ADSPREQGLMMESLMAAIAQQSPEAKERLKLFLEGKAPKLGGA"
     gene            5029..6843
                     /locus_tag="PKM27_07395"
     CDS             5029..6843
                     /locus_tag="PKM27_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HNR07123.1"
                     /translation="MTRIESPVPFRPVETIAYDLRMEQRPDGSFLVQSNIPLEVHPVR
                     MTERFCHWAEVSPDRIFLAQRDDHGTWRTLTYHDAYGQMLGIASWLLTRDLSEGRPLA
                     IWSENSIEHALLALAALHIGLPYSPISPAYALRSKDFNKLRQVMDLLRPGMVLVSDPT
                     RYDPALKAAANGLTVLASKTSPDHKGLLTLDQIAKTPVDPKVESAYQSIRKDTVAKIL
                     FTSGSSGNPKGVINTHGNITANWQQISQTFPFLKEEGLEYVDWLPWNHTFGGNHNFGL
                     ALYHGATLYIDDGKPTPEGIGRTVRNLTGRMPTVYFNVPKGFEELIPYLRNDETLRRQ
                     FFSNLKMLFYAGAGMPQHTWDALEELSIDTIGQKILIGTGLGCTETSPSALFASRPGG
                     FAGLLGVPVPGLTLKLVPTGGKLEARYKGPNVFPAYWSQAELTRDAFDEEGFYRTGDA
                     LKFFIPGDPESGFLFDGRLTEDFKLSTGTWVHAAQLKARIIAAGAGLIQQVVLTGSDR
                     PYIGAIVFVNPAEVKKMLKDEFDPLNYTKHPWLIARINSLLSGLNSESTGSSNRIERM
                     ILADMDLSLDRGEITDKGTINPAMIIRNHPELVDQIYG"
     gene            7450..8211
                     /locus_tag="PKM27_07400"
     CDS             7450..8211
                     /locus_tag="PKM27_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR07124.1"
                     /translation="MNIHNISAIVTGGASGLGAATARLLAKAGARVAICDLNMTLALD
                     LANQINGLAIHCDVADETSARGAFELVKSTHGPARILVNCAGIATAGRIVGKQGPSPL
                     QDFEKTIRINLIGTFNMLRLAAADMMTLEPMEEGERGVIVSTASVAAYEGQIGQSAYA
                     ASKGGVVSLTLPAAREFAQFGIRVNAIAPGLFMTPLLGGLPEEAIHNLSASIPFPKRL
                     GLAEEFAALVKHIIENRYINGESIRLDGALRMQPK"
     gene            8237..9355
                     /locus_tag="PKM27_07405"
     CDS             8237..9355
                     /locus_tag="PKM27_07405"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HNR07125.1"
                     /translation="MMKNKINPPFRADQVGSLLRPPHLKESRLKWKRGEITAEALREI
                     EDVAIREVVKMQESIGLHSITDGEFRRDYFHLDFLKELSGVSVTGGIEANPHARVAED
                     GFTPPKLSVTGKLKHVKDIQVADFNYLKSVTSQTPKVCIPSPTMVHFRGGRKSIDIEA
                     YPDMDLFFHDLSACYKAEIDALYRNGCRYIQFDDTNLAYLCDPKMRAAAVERGDDPNE
                     LPRTYAGLINSVIDDRPEDLRVAIHLCRGNYRSTWFAEGGYEPVAEILFNAIHVDAYF
                     LEYDDERSGDFAPLRFVPDHKTVILGLVSSKINTLEDPVDLIRRIDEAARYLPLENLG
                     LSPQCGFSSTHHGNALSQDEQWRKLELVVKTAARVWPV"
     gene            9536..10396
                     /locus_tag="PKM27_07410"
     CDS             9536..10396
                     /locus_tag="PKM27_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR07126.1"
                     /translation="MNTNSLHAIDFHTHAEVSCRQPHDGYRPELDEAFTRYFKSDHRP
                     TIQETVDYYRSLNIALVMFAIDAEFELGRKRIPNEEIAEAAAANPDILIPFASIDPHK
                     GKMGAREARRLIEEFQVRGFKFHPTVQGFYPNDRMAYTLYEEIAAADLPMLFHTGHSG
                     FGSGARAGGKLRLEYSNPIHLDDVAIDFPDIPIILAHPSWPWQDEALSVCLHKPNVYI
                     DLSGWSPKYFPPQLIQYANTLLKDRMLFGTDFPLITPERWLKDFETAGFREEVKPLIL
                     KENAMRVLGL"
     gene            complement(10437..10829)
                     /locus_tag="PKM27_07415"
     CDS             complement(10437..10829)
                     /locus_tag="PKM27_07415"
                     /inference="COORDINATES: protein motif:HMM:NF036886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5618 family protein"
                     /protein_id="HNR07127.1"
                     /translation="MNPVIREANRHLANAKEILVQKAKRTNGIYKDKKYVKMAGHTAY
                     CGVLHALDQLIMPSEKSRTQKNIRKSVDYYRSLLAKYDKKMLAEFNNLYDTLHLSLGY
                     DGNTDAGIAKRGLEMAKEFIDKVATRLN"
     assembly_gap    11342..11441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11442..11757
                     /locus_tag="PKM27_07420"
     CDS             <11442..11757
                     /locus_tag="PKM27_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07128.1"
                     /translation="LFKCNILPFQPKASKEEIVNLTAKFASKPKYRNDISLPFSLCAS
                     SASSAPSAVYLNEIFFLSNQKHRRKKLFINRQVRRGSAKTFHYPFSFAPHLRPPRPLR
                     FI"
     gene            12106..13029
                     /locus_tag="PKM27_07425"
     CDS             12106..13029
                     /locus_tag="PKM27_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNR07129.1"
                     /translation="MKQVLQLLPFILSLLSLQGHAQHIQLFRYNSPEGAKPAVRTSEG
                     AMLDVSGYTTDFNERFFEAGGIPGLVKWLEKNRKKCKPVPPSFTYAPCVARPSKIVAI
                     GLNYLKHIQESGASVPSEPVIFFKATTALCGPNDPLIIPKNSVKTDYEVELAIVIGKK
                     ASSIHEEDAPGYIAGYTIINDYSEREWQLEKPGLQWDKGKSADHFAPLGPYLVTPEEV
                     GDPHNLNLWLKVNGEMRQNSNTGDMVYKVARLVSEVSKYMTLLPGDVIATGTPSGVGQ
                     GFKPPKFLKPGDVIELGIDKLGAQKQLAVSK"
     gene            13092..13607
                     /locus_tag="PKM27_07430"
     CDS             13092..13607
                     /locus_tag="PKM27_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07130.1"
                     /translation="MKIILSLLALCILFTNCSQQLPVTHTVDFETEKAAIQAVIAKET
                     ESYYKQDYDAWKSTYLQSSAFRKFGYWEGYPEKVTSNIGFESLAAEKKKQFDANETLW
                     RGSTEERSNENFRISNEMAWYTFEQNSYEKDTRKFLGKSLETRILEKENGEWKIAFLG
                     FHYYPMEEENK"
     gene            13694..14602
                     /locus_tag="PKM27_07435"
     CDS             13694..14602
                     /locus_tag="PKM27_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNR07131.1"
                     /translation="MKQKVSLVLSGGGAKGIAHIGVIEELEKQNFKIASLTGTSMGAF
                     VGGIYALGKMEEFKKWLFALDKLKIFKLFDFSFSSQGLIKGDKVINELKKFIPDQNIE
                     NLNLLYAAVAVDLLNKKEVVLDKGSIYEAMRASIAIPTVFTPVKKDNMLLVDGGVLNN
                     IPINHAKRSPGDLLIAVNVNADIPLYHPSLLKEEFETKQSIYRKKIREFQIQLQKIIP
                     PNKDESLDYFDLINKTIHLMIERITLTTIEKYSPDILINISRNSCGTFDFYKAEDMYE
                     IGCYSAKKYLEEYKKSYEQRRISETK"
     gene            15011..15700
                     /locus_tag="PKM27_07440"
     CDS             15011..15700
                     /locus_tag="PKM27_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNR07132.1"
                     /translation="MIGILIQLAVSWILLWLMDKKDLTVLGLKPTTDRLRKFSFGFII
                     SAAWFTVYCQTTTFLTNNHWTINQDFSLKEFLSSTWWTFKSVIYEELIFRGAILFLLI
                     QKTNIKAGCIISSVAFGIYHWFSFGVLGNPVQMTYIFLWTGIWGLMFAFAFAKTRSLY
                     LPTGLHLGWNLLNIVVFSQGPLGNQFLIHSNNGQGLTVFPSVGLQIFQLLGLPIMVYL
                     YLKNYRQSDHK"
     gene            15745..17445
                     /locus_tag="PKM27_07445"
     CDS             15745..17445
                     /locus_tag="PKM27_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNR07133.1"
                     /translation="MQQNNFDAIVIGSGISGGWAAKELCEKGLKTLVLEKGRPVVHVR
                     DYPTANLDAWDMEFRGALTREEAKRFPVQTRSGFVGGDNKHFFTDDLDNPYIETQRFD
                     WIRGNQTGGRSLTWGRNCYRWSDLDFEANKKDGIAIDWPIRYRDIEPWYTYVEKFAGI
                     SGEKLGLSHLPDGYFLPPIPLNCIEQHLKKEVEGKFKGRHITPARVAHLTDPQPWHLE
                     LGRGKCMNRDRCARGCPFGAYFSSNSATLPAAARTGNLTLRNHAVVHSILYDEQSGRA
                     TGVRVIDAETMISTEYRASLVFCCASTLATTQILMNSLSSRFPNGMGNDSGQLGRNLM
                     DHHYRCGAYGEHEGYADQYYKGRKPTGLFIPRYVNLDRLTENKNFIRGYDYQGAGGTR
                     NPWSRGIQEPGVGAEFKESLFKPGGWNMGLMGFGECLPYEDNRLSLDPEKKDKWGLPL
                     LVIDAGFRENEMKMREQIKADAAEMLEVAGFKNVRVFDEIGGMGVGVHEMGTARMGRD
                     PKTSVLNGNNQVHGVPNVFVTDGACMTSASCVNPSITYMALTARAVDFALQALNKRNL
                     "
     gene            17456..18049
                     /locus_tag="PKM27_07450"
     CDS             17456..18049
                     /locus_tag="PKM27_07450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HNR07134.1"
                     /translation="MNRREAIKNQSLVIFGVAVSASAWSGLYLSCRSMPVLDWKPGFF
                     TVEEASLLTAITETILPKTQTAGAMETGVPRFIDKAIAALFDPETQQKIRDGLDQIDR
                     MAKTTFGKKFPSCSEEERTSLLLDLDRKAGTYPLSLWGITLEQSKPVGFFRDLKSLTL
                     MAYFTSEEIGKGVLRYDPVPGDYQACLPLDGMNSWTE"
     gene            18102..19865
                     /locus_tag="PKM27_07455"
     CDS             18102..19865
                     /locus_tag="PKM27_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR07135.1"
                     /translation="MKGFSIIICFFLLSLNISSQQVIPLYSGSAPGSESWTHQEKTDT
                     LRGPNNYIFVSNVVNPSLIVYRPEKPNGMAMIVCPGGAFRALSIQSEGTDVARWLNTK
                     GITAFVLKYRLVPGHIASREQAMEDIRTQNSRRIDSLNAPYVPLAVADGREAVKYVRT
                     HAAEWGLHPNRIGIMGFSAGGTVAASVALTPQSSILTPQSSPLTPQSMFRPDFAAPIY
                     AYCGAIEGNQVPADAPPLFLAFSQDDFISDGNPALYQKWKAAGRPAEMHVYPDGGHGY
                     GIRKQGKASDGWENRLAEWLNYQFPVQEFGSFWKDLDYVGDQIPGHKLDIHLPFNKKG
                     PFPVMVAIYGSAWFSNNAKQNVFRENLGRRLLEAGYAVVSINHRSSRDALFPAQIQDV
                     KAAIRFVRAHAGKYQLDTSFIGITGWSSGGHLSALAGVTGDIRRYTLAGGTMDLDGSI
                     GPHTGMSCAVDAVVDWFGPVDFLIMDSCGSSFSHNGPYSPESFLMGSPIQEIKTKVAF
                     TNPVHYIHPGTPPFLIFHGDQDFQVPACQSQKLHEHLIMKNIQSESIVVPGGGHGPGV
                     MIDYYYDKMIRFLDGELKKGK"
     gene            20161..21807
                     /locus_tag="PKM27_07460"
     CDS             20161..21807
                     /locus_tag="PKM27_07460"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07136.1"
                     /translation="MKNKLILLIAIYTLTSLILQSQDVFKTQRTAWLQKAEALKPKLA
                     QVKVKPVHAITTRPDRGAFQGWRVDRKWSTDSLFTKSFKNQTGLILDFGQHLTGYFTM
                     KLEEIRGVADAPTRIKLTFGEVPSEVSVPFEPYNGDLSRAWLQDEIVTVSEMPSEFTL
                     TRRVSFRYVKLELLGASRFFDFKIADAWCMAGTSAAAVPPPLKPTTYEAIAKIDSIAR
                     NTLKECMQTVFEDGPKRDRRLWIGDLYLQSITNNHTFKNHNLTKRCLYLLAGLSTEEG
                     YLPSNVFERPGPHSQKGAPFLFEYSLLYNATLLEYLKATNDRTTALDLWPVAKKQLDN
                     PKKFMLDNGLFNDTAAAANRWWLFIDWKEGLDKQAPIQGLIIFALNQTVELAKMLGKQ
                     NEVAEIPALVEKMKKACMDHLYDKDLGLFVSGPGKQVSFASQAWMVLAGVTDTAESQK
                     ILTYVKEHPTAVKPGGPYLMHYYVEALMRSGLTSAARQQILSYWGNMVIKGADTFWEV
                     YDPENDYASPYKFYPVNSYCHAWSCTPAYFIRKYPEVFQQ"
     assembly_gap    21945..22044
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22641..23630
                     /locus_tag="PKM27_07465"
     CDS             22641..23630
                     /locus_tag="PKM27_07465"
                     /inference="COORDINATES: protein motif:HMM:NF005147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine dehydratase"
                     /protein_id="HNR07137.1"
                     /translation="MKFLSIEDISKANERVASLVHKTPIFTSSLLNQWMGHELYFKAE
                     CMQKIGAFKARGACNTVAWLLENNEKPKHIVANSSGNHAQAVAWAASRFGIPSTIFMP
                     AYSSKVKIQATKSYGAEVILCETRAVVDEKVKLASEEEGTCWIPPFNHDQVIAGQGTA
                     AYEAIEEVGEVDGVFAPCGGGGLLSGTWVASRGLAPKASVIGAEPLNANDAAQSLRMN
                     SIQKLQSVPDTLADGAMTIAVGDLTFEYLKQLDDFYEIEESRIRYWTQWLTHLLKVRV
                     EPTSAMAMEAAFRWLSKQTSKKRALVILSGGNIDQETSLKIWKDNCLDEVPRL"
     gene            23736..24809
                     /locus_tag="PKM27_07470"
     CDS             23736..24809
                     /locus_tag="PKM27_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR07138.1"
                     /translation="MFSPLQLSIRFQYLVSILLIFLVSGVSYYLTDYTGYRVVALVLL
                     MTVSVLAMLFRILPVLLAALLSALIWNYFFIPPRFTFHIDHTEDALMFLLYFLIALIN
                     AVLTIKIRQAESRARDKEEKETTIRLYNTLLNSLSHELRTPIATILGSVDTLKENRDK
                     LSEKNQAELLTEIDTAGIRLNRQVENLLNMSRLETGLLKPKPEWCDLNELVHRVIRKY
                     ENPETCTLRFEPDEMLPLLKIDGGLLETVLDNLVQNAIQYHPGHALVDLSVRYESNTC
                     LITVSDHGAGFPEEEIPRVFDKFYRLPDSKTGGSGLGLSIVKGFVEALSGKVSLENNK
                     EGGAKFSLSIPAEASYLKNLKNE"
     gene            24802..25479
                     /locus_tag="PKM27_07475"
     CDS             24802..25479
                     /locus_tag="PKM27_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR07139.1"
                     /translation="MNKEEILIIEDEAQIRKLLEILLESNDYKVWQALSGKEGIVLAA
                     NHPPDLILLDIGLPDRSGHEILKELRTWYNRPILMLSVQDKEEDIVSALDHGATDYIT
                     KPFRAAELLARIRSALRRSQPGPATACITCDDLQIDLAARTVKKNNEPVRLTSTEFNL
                     LALFVKNEGRVLTHHYLLKEIWGPGFQTETQYLRVFVGSLRKKIEDNPNQPRHLITES
                     RVGYRFI"
     gene            25626..27596
                     /locus_tag="PKM27_07480"
     CDS             25626..27596
                     /locus_tag="PKM27_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNR07140.1"
                     /translation="MEKPKNGSYHKVTAATLLVALGIIYGDIGTSPLYVLKAIAGDRP
                     VSETLIYGGISCVFWTISFLTTFKYIYLTLKADNHGEGGIFSLSALVRRYGKYLAIPT
                     ILGATTLLADGIITPPISVASAVEGLTGLKGLENLPTVPIVIVILSALFFFQRFGTHK
                     VGSTFGPAMLVWFTMLSTLGMIQIFRHPGILKSLSPVYAFRFLTEYPHGFWLLGAVFL
                     ATTGAEALYSDLGHCGRKNIRITWVFVKTCLMINYMGQAAWIMNLGEGQFLDGRNPFF
                     EIMPEWFLIPGIVIATLAAIIASQAMISGSYTLISEAMNLNFWPRIAVRQPTELKGQI
                     YIPSVNGILWMGCILMILYFRSSTHMEAAYGFSISITMLMTTFLMIHFLRYKLKWNKW
                     FVFAVFLLFAAIESSFFIANAAKIKERWMFLFFELFIFLVMYIWYYARKVNNRFTRFV
                     ELGQHVPAIRELSRDEDIPLFSTHLVYLTKANNRHEIEEKIIKSIFARKPKRADVYWF
                     VHIHRTEEPYTLTYSVTELVDDQVIKINVNVGFRIQPRTELYFKKIIQDLVANQELNL
                     HQRPDGSNKYNHEPSFKFVVIEKYLSVENEFSFREGLLLKTYFLLKRVSLSDEKAFGL
                     DKSDVLVEQVPLIYQPATRFELKREPAKPIQA"
     gene            complement(27863..28555)
                     /locus_tag="PKM27_07485"
     CDS             complement(27863..28555)
                     /locus_tag="PKM27_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07141.1"
                     /translation="MILISFIGWCMLLGKPAIPGSHDASSCFKPTAPPSCFEKYADRL
                     DEWLTREDIVSIFPGLPAEAAINYSRNVPIAYRSSVYQWKSDRFSISFVGSREVRSPR
                     PNKIGVGHLTVYGDRVKDPLTYFRNAHRTPTAEEKSELQQRMKTKMEEKGLTSSQKGA
                     GTTLTAALSEQILFTNVEGLGDAAAWDHMDHSLVILVGRVRFKVFVEISGNVEDNQEL
                     AIKLAKKILEKC"
     gene            complement(28615..28941)
                     /locus_tag="PKM27_07490"
     CDS             complement(28615..28941)
                     /locus_tag="PKM27_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07142.1"
                     /translation="MRFPQLIFSGLLFITISSCSQSTDDSNCSASELSQKEQAMLDAQ
                     NLSISAQIAHAQDDSAENCMAYKKAYLEYLDKFTLWINCLDESTKEQWRPVYAQAEQK
                     KQDIPC"
     gene            29118..30077
                     /locus_tag="PKM27_07495"
     CDS             29118..30077
                     /locus_tag="PKM27_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR07143.1"
                     /translation="MKTVCIIFFHLFLTWSVLGQMVLPLYPGNIPNNKEVSGISDTAN
                     TFMVDHTVKRFITRITKPELTLYLPDKKKNSGYAVLICPGGGYHGLAIDHEGHDMAKT
                     LVEAGIAGIVLKYRCPKPEYVVNKEIVPLQDAQQAMLLLRTRAKEWGIRENRIGILGS
                     SAGGHLASTAGTHFLDQKIENLKGLSLRPDFMILNYPVISFADSVTHRGSRNNLVGTP
                     PYDSFDPEKIAYYSNELQVNPTTPRTFISHAEDDKTVPIANTRLFISALEKNHIPVTA
                     VIYEKGGHGFGMINPTSDRDWMKECIQWIKKGGKEKNKKYCSM"
     gene            30098..30817
                     /locus_tag="PKM27_07500"
     CDS             30098..30817
                     /locus_tag="PKM27_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07144.1"
                     /translation="MKSIIDYIPIVTTFFSIYFTVVIYRHYQLRRTPYLLWWTIGVLT
                     FGLGTLAESIHALAGWNEINLKYWYIIGALLGGFPLAQGTVYLLMPRWFARISCYFFV
                     TLILVASVCVMLSPITIPEDFNGKLTGAVFSWKWVRSFSPFINMYAFFFLVGGALYSA
                     FKYFTSGERKALFKGNILIALGGLLPGIGGTFTRMGYVEVLFVTELIGLYLIYLGYRT
                     IKKEKQLLQNSATDKGLFLGS"
     gene            30933..31277
                     /locus_tag="PKM27_07505"
     CDS             30933..31277
                     /locus_tag="PKM27_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNR07145.1"
                     /translation="MAYDQKLAHRIRHRLSGLPNVEEKEMMGGLTFMVNGKMCAGIIK
                     DALMCRIDPEFHDLALEKQGCRTMDFTHRPMRGYVLVDESGMHSDKDFDYWINLALEF
                     NKKAKASRKKKK"
     gene            31356..31649
                     /locus_tag="PKM27_07510"
     CDS             31356..31649
                     /locus_tag="PKM27_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07146.1"
                     /translation="MNNERTPHILNTSATLLGLCFIVLTSRNVLPEAEITVIDYLTGL
                     AILGFMAACLLSYMSMRKGEKSKWPLETAADSVFIAGLFFLFAATLLIVLNII"
     gene            complement(31771..32646)
                     /locus_tag="PKM27_07515"
     CDS             complement(31771..32646)
                     /locus_tag="PKM27_07515"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNR07147.1"
                     /translation="MAERSIEIVVPPREPHYVGDGFRVHNFIPSGYRLDMERMDPFIM
                     LDYGSPHYFPPSDKPRGVSVHPHRGFETVTIAYKGRVAHHDSAGNSGVIREGDVQWMT
                     AASGILHKEYHEEEFSRSGGDFQMVQLWVNLPAEFKMSPPKYQALSHHQLGKHILSEG
                     QGMVEVIAGKYEETQGPATTFSPVHLMNARLNQGAEADFSFPAWFATALLVIEGKVQV
                     NEKEEVPANHFVLFGTDGDSFSIQALEPSVVLVLSGQPLEEPIAAHGPFVMNTREEIA
                     QAFDDFRKGKFGYLE"
     gene            32783..33955
                     /locus_tag="PKM27_07520"
     CDS             32783..33955
                     /locus_tag="PKM27_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HNR07148.1"
                     /translation="MKTSVAIIGAGPAGLSLALLLHQAGIPCLILERHSRAYVESRVR
                     AGLLEQNTVDLFTELGVAGRLRREGLEHFGVILSFEGERLRIPFDELTGGRHITIYGQ
                     QEVVKDLVAACLERQIEIRFEAKAWELHRIETEAPYIQYIQDGTRKSLACDFIAACDG
                     FHGIGRKFLPPGSYIDYQTSYPFSWLGILAETAPSSEELIYTFHEEGFALHSLRSDAI
                     SRFYLQVDNTEKLEDWDDSRIWESLEKRLFTPGFKLQTGRILEKSITPVRSYMTGTMQ
                     SGRLFLAGDAAHIVPPTGGKGLNLAVADIRWLAKAMIAFYKSGDEQKLRSYSQDCLRR
                     IWRVQDFSHFMTQLFHRIGERESLEYRLQRSKFDYLKISRAYQTSLAENYVGLKEI"
     gene            33958..34746
                     /gene="pcaD"
                     /locus_tag="PKM27_07525"
     CDS             33958..34746
                     /gene="pcaD"
                     /locus_tag="PKM27_07525"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HNR07149.1"
                     /translation="MPFCVCRGHLIHYKYLDQGKNQTLVFIHSLGTDFRIWDIVAGNL
                     FPVANILLFDLRGHGLSGLTPSPRGLEDFTEDLLGLLEYLGIRQQIIPVGLSVGGIIA
                     QLLIHRIPDQIPKVVLCDTRYKIGNAQNWNDRIAQVRRAGIESISEGVVSRWFSKDFH
                     ARFPETVLGYKYMLERTPVDGYIQTCEGLRDADTGDIARKIAVPALCLVGAEDLSTTP
                     EEVKNLADLIPGSVYLVLENSGHLPCIDNAAKLSRLITDFIQES"
     gene            34743..35129
                     /gene="pcaC"
                     /locus_tag="PKM27_07530"
     CDS             34743..35129
                     /gene="pcaC"
                     /locus_tag="PKM27_07530"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR02425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HNR07150.1"
                     /translation="MKDHLYEQGMHTRRSVLGDDYVDHAESVKTEFDSDFQDFITRNV
                     WGSVWSRPGLTKRERSLVTIALLTALGHFEELTFHLRASRNTGASLEEVKEVLLHTAV
                     YAGVPAANHAFKIAKKVFENETGSSS"
     gene            35156..35842
                     /gene="pcaH"
                     /locus_tag="PKM27_07535"
     CDS             35156..35842
                     /gene="pcaH"
                     /locus_tag="PKM27_07535"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HNR07151.1"
                     /translation="MSHSSGRDWTVHPPFLYPDYKSTVYRAPSKPLIPLKHRLGELSG
                     PVYGEEVVRLHDNDLTKNAAVNGEPLGQRIIVAGRVTDELGRPIPLTLIEIWQANAAG
                     RYLHKTDQHPAPLDPNFFGGGRCLTDEEGYYKFYTIKPGAYPWGNHANAWRPNHIHFS
                     LFGRNISNRLVTQMYFPGDPLLDFDPVFKSVPEAARHLLISRFNMDFTEEAFALGYEF
                     NIVLRATPFE"
     gene            35927..36502
                     /gene="pcaG"
                     /locus_tag="PKM27_07540"
     CDS             35927..36502
                     /gene="pcaG"
                     /locus_tag="PKM27_07540"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HNR07152.1"
                     /translation="MPRLPQTPSQTVGPYFAYGLTSQQYGYPFPSLNDGMMIDDSIEG
                     ERITIRGRVFDGLEESIPDAMIELWQADARGQYLQTGLGRQLLFGRQGTGSLESREFV
                     FQTIKPGSSAGQAPVIHVSVFMRGQLSHSYTRIYFAEEVALNSRDEVLLSVPPERRST
                     LLVGRSVIHGMPVYHFDIHMQGEKETVFFDV"
     gene            36808..38148
                     /locus_tag="PKM27_07545"
     CDS             36808..38148
                     /locus_tag="PKM27_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HNR07153.1"
                     /translation="MKTVPILSLESAVSLVKSGDRLLVGGFGMTGNPVHLLHALAETR
                     VKDLTYIANNTGEPGLGGGRLLRNGQIKKIIGSFFTSNPDAVAAAQEGRVEYELLPQG
                     SLAEAIRAGGAGIGGFYTPASAGTLLARDKETKWIDGVEQVFIKGIRAEVAFIRAWRA
                     DTSGNLQYRMTEQNFNKAMATAADLVIAEVEEIVPVGHIAPEDVHTPGCFVDYLTHAH
                     LTQEDLGISASLQSSKKENDLRTLIAERAFRELKRGDVVNLGIGIPTLVADFISPDDG
                     IILHSENGMLGIGPSPKDGGGAMHYPVNAGKIPVTALPGSSYFDSADSFGMIRGGHID
                     VAVMGGLQVDEKANLANWAVPGKPLLGVGGAMDLAAGAKKLMVLMTHTHPDGSPKIVR
                     ECTLPLTAKNVVTMIITDLAVFVFREGKLTLAELMPGHGLEEVREKTEAEFVED"
     gene            complement(38269..39057)
                     /locus_tag="PKM27_07550"
     CDS             complement(38269..39057)
                     /locus_tag="PKM27_07550"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR07154.1"
                     /translation="MPEQKERLLMKNYNPETYWSAVANRIELRDGGPFLAGDDEPYYR
                     YKRNLFLQMLNKIEFKDKSVLEVGCGPGGNLIEILKHQPRELCGVDISEKMLEVAKRN
                     LPPPVKLVKSSLGLLPFGDGTFEVVFSATVLQHNLDEGQFSKLLEEMARVCSGKIYLF
                     ERVEKATYVTDYCIGRPLNAFADILKPRGYQLINAENISIKASYIMAGVIRKLFNRRS
                     RAEGENLSEITIKLENLLLPITSKLDRLLKLKSDLTKMEFVRTN"
     gene            39197..41428
                     /locus_tag="PKM27_07555"
     CDS             39197..41428
                     /locus_tag="PKM27_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07155.1"
                     /translation="MKGLLSKRQNRIDIGLWTAFLFLFSTSLLSQVRIAGNLQNETIR
                     YGLKRLSEQTAISLDETLDLQKSPGRRPDLLVLAPDDLKNYLYVGIQAYEFDAVKTEG
                     FRFKWLSDHKTLCLLHRDPVGALYGILELKESLESGKAWSSLPEKTSNPHLEYRIIKF
                     NLPWSPYRNNEAVTLHTSTCRDLKFWEKFLDRMVDNRWNALSLWNIHPFPFMIRSKSF
                     PFANEFTEQEMQDWKQFWTSLFEMAGTRGIQTFVINWNIAVSPSFAKNYGVPEYSSLS
                     DTVIRYTRESVTQLIDEYPGLTGIGVTLADWMGTFDEKMTPQQREDWIEKTFVAGMQA
                     ASRPVKFIHRSVLAGDPMAMRALLEKAKLKDPALVEIKFNWSHGHSTPRLAITHDYHS
                     GKLDERFWTPKPEDYHIQWMIRNEDFFILRWGQADFIRQHIHQNSKDYVNGYFIGSEG
                     YVPALDYYTIRDGQDAPQYAFENQWLFYTLWGRLLYDPALPDEFFIALLDKRYGKGSG
                     KTLWQAINLGSKIPLRLASFFRSTWDYTLYSEGFLAPEPSNPDGFFDRTSPFISIDEL
                     IHHETLDPKMISIEDYSKLKKENKKAPDSLISPLQIARQSEKDSKKALRGYKAWTKRN
                     PSAAPPLASEWVDAATWARLGLYFAHKLRAGVALHSFRQTGRISEKHKAIRELESALV
                     QWKEVALLTKDRIKATPHVALGRYRDTFPLFSWQALLPQVERDLQIARDAQKGLFPQT
                     KTQ"
     gene            41533..42786
                     /locus_tag="PKM27_07560"
     CDS             41533..42786
                     /locus_tag="PKM27_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR07156.1"
                     /translation="MPKPSSFSTYHKIIIFLLALTQFTIVLDFMVMSPLGDFLIKAMH
                     ISPGQFGIAVSAYAFSAGISGLLAAGFADRYDRKKLLLFFYSGFILGTLACGLATTYP
                     FLIFARILTGLFGGVMGSVLMAIIGDVFKPELRGRVMGFIQMGFAASSVLGIPVGIYL
                     ANIWDWHAPFLMIVGLALLIFILVYRFMEPVVHHLSQNQKTTALNHFRNVLAHPPYQL
                     AFLATALLSIGGFLMMPFGSAFAINNLKISPDQLPILYMATGIASLVIMPLVGRLSDR
                     TNRFKIFLFGSVWASIFIVIYTNLPVVPLWAVIVLNILLFAGIMSRMIPASTIMISLP
                     RLHDRGAFMSISSSLQQIAGGIAAAFAGLIVTQKTPNSPIEHYPTLGFISVAVMMLCA
                     YLIYKVYLMTRQASKEGVSQAKIEQ"
BASE COUNT        10702 a        11227 c        10148 g        10643 t
ORIGIN      
        1 ccggcggcct cagcgggaaa ggattttatt ttgaaaatcc caatgcgtcc cggacctgtg
       61 ggtgtgggga gagttttgcg gtttgatttt gttggatagc ccaatttatt tgggaggagc
      121 tgagttctgg ttatccttcg attaaaaaca attttaaatt cttgcaattt aatgcccttc
      181 ggttgggcct caccggagtt tagtttggga ggagctgagc tccgaccctt taattatcaa
      241 tggattaaaa tattttttat acttaccact taacgccatc cttggcggtc ctgcacatta
      301 gcagggatat ggctttgcat aaaaagggtt tgacataata ccagtaattg gttattcttg
      361 tataaatctc attctatggc cagaaatact tctatactcc taggtgatca ttttgaggag
      421 tttatttcat ctgaaatcgc ctccggaaaa tacagttcgg ccagcgaggt ggtccgtacc
      481 gctttgagac ttcttgaaca ggaggaggaa aaaaggaaaa tgcttgttca ggctcttgaa
      541 cagggagaga aaagtggttt tattaagaat tttgacccta aggcccattt gcttaagttc
      601 catggcaagg tcaaatgaaa gcactggagt atattataag taaaaaagct attactaatc
      661 tcggggatat cttgctttat gcagccggga aagagtctgt gacacaggct gaccggttat
      721 tattctcttg ccatggttga gattgagtat aaatgccgta atgtggagtc cgcaaagtcc
      781 aaagaacagt tgcgaaaagg gtatcgggct tcaaaagtca aatcccgtat tattttcgat
      841 agaaattcat ccgatgcaat tgagattgtc agggtattgc atgagcggat ggtcctggag
      901 acctatctgg ggggtaatca acggccaaaa caaggatctc aatattgaat taaagcttcg
      961 gggcttccct aagtgtttgc cacatactgc gcttagggcg tgcgcgaagt gacaaccact
     1021 ttgcgacgct caagcgcctt actccatacc agctttcctt atattcgtca gcgcttgcga
     1081 cgtaagccgg cagaaaacat ggaaacgatc aatcccgcac caccctccct ccgatacgcc
     1141 tggatggtcg tagccatcct catgctggcc aacgtgtctt ccttcatcga ccggcagatc
     1201 ctaagcctac tggtccggcc gatcaaaagg gatatggggt tgtccgattt tgaggtcagc
     1261 ctgctcatgg gactgagctt cgcgctcttt tatacctttt tcggaatggt gatcggaagg
     1321 ctggctgacc agtacaaccg cagaaatatt atcgttgcag gcattgccac ctggagcctg
     1381 atgacggccc tctgcggcag tgtgcggacc tatggccagt ttttcctggc ccgcatgggg
     1441 gtgggcgtcg gagaagccac cctatccccc tccgcttatt ccatcatcag cgattatttt
     1501 ccaaaaaaca aactggcctc cgccctcagc ctctattccc tgggcatttt cctcggttca
     1561 ggtattgcca tgctgatcgg ggccggggtc atcgcccgct tgcccaccga ggggatggtc
     1621 cggatccccc ttttcggaga ggtcttcccc tggcaattgt tattcgttta tatcggcctt
     1681 ccgggcctgt tgatcgccct gcttatactc cgcgtgaaag aaccggtacg caaaggtctg
     1741 ctgctcgacc aggggcaggc caggaatttc aacacccgtg aagccctcaa aaccatttta
     1801 aataaaaagg cggcgttctg gggggtttgc ctgggagcct ccttttccgc actctgcagt
     1861 tatggcctca acgcctgggt ccccacctac ctgcagagga cttttgactg gtccatcccc
     1921 aaatccggcc tgtattacgg aggggtggtg gtcgtctcct ccctcctcgg ggtgctttgg
     1981 ggaggctggt atgccgacaa actgatccat aaaagcatcc ccactggacg gatccgggtc
     2041 ggtattattt ccacggcggg agtattgctt tcgtttccga tacccctgat cccggatggc
     2101 caccttgctg tgctggcttt tgcaataccc ggctttttca tcgcttccaa tatcggcatc
     2161 tccgcggcag ccatccagga gatcatgccc aaccaggtca gggcctttgc ttcttcgatt
     2221 tttttatttg ttctcaacct cattgccctg gccatgggcc caagccttgt ggccgccttc
     2281 accgattttg tatttaatga tgagggcatg ctgaaataca gcctggtatt tttattgata
     2341 ctagggggga gcctgggagt cggcggcttt ttatggtcga ttaaaaacta caaagaagaa
     2401 ttgatccttc atcacgccca tctcaattcc ggtaaaccat gaagaaagga gaactgtttt
     2461 cacgacgcaa tctggacttc ctcttgtttg agactcataa aatttccgaa ctgctcgggc
     2521 attcccctta ccaggcccat gaccgggaaa ccataggact agccctggac accgctgccg
     2581 atatcgccga aaggcacatg aaacccgcct ttgtggactc ggaccgcaat cctcctttta
     2641 tccgggacgg ccaggtcatc gttcatccgg tcacccatgc tttttatcat tccttcagcg
     2701 aaagcgggtt attggcctcc acctttcccg aatccttagg agggatgcaa ttgcccaggt
     2761 cggtcttcgc ggcgatagac tttatcatcg gcagcgccca caatggtttt caaatgtaca
     2821 ccagcctggc tgtgtccgct tccaggctga tcgaaagctt cggcagtcag gatcaaaaag
     2881 atctttatct cccccgcctg ctgtccggag actgggcggc aacgatgtgc cttacggaaa
     2941 gccaggccgg cagttcactg agtgatatca cgaccaccgc ctttcttcag ccggatggct
     3001 tttataagat aaagggccag aaaatattca tttccgccgg cgatcacgac atcaccgaaa
     3061 acatcctgca cctggtgctg gcccggatcg aaggcgcgcc caaaggagtg aaaggcctct
     3121 ccctttttac agtccctaaa aaatggctgg gggattccgg cgaattaaca gacaacgaca
     3181 tcacttcggt aggcatcttc cataaaatgg gccagcaatc cacaccggcc ctgcacctgg
     3241 aattcggcgc cggaaatcat tgcagaggac agctcatcgg ggaaccaaac cagggactga
     3301 aatacatgtt ccaaatgatg aattcttccc ggttggccgt ggggctcgcc ggaggctata
     3361 tcgcttctgc agcctactac acctccctgc aatatgccag ggaaaggatc cagggccgca
     3421 acctggaaga aaaaaatccc ctgtccgaac cggtgcccat cataagccat cccgatgtca
     3481 ggaggcttct tttcaaacag aaggcgttta ccgaagcatg cctgtccctg gtgattcagt
     3541 gccagcttta cctcgaccgc cttcaaaccg gtgtccccgg cgaagaaaaa aagaaatacg
     3601 aacaattgct ggaattgatg acccccatgg tcaaagttta tggctcggaa gagggcaatg
     3661 actctgtcca tgcgggtttg caggtcctgg gcggttatgg atatacccgg gattttatcc
     3721 tggaacaact ggcccgggac gttcgcatcc tgtccatata cgaaggcacc acggcgatcc
     3781 agggacagtc ccttttgggc cgccagattc tgtcggggga tggttcggta ctggatcttt
     3841 ggcagatgga aaccggtcaa gacctgggga tggcttctgc tcacccggat ttaaaaccct
     3901 atgtcctgaa cttgtcggaa aacatcacgg gttttatttc agtgagcagg gaattgatcc
     3961 gaaaagcaag agaaaccagc gcggtgcctg ctctggccga tgccagcctc tttgtcgaat
     4021 ggtttggatt gctcaatgcg ggatggcaat ggatcaaaca ggcgtatacg gctctggttg
     4081 ccctggaaaa agccgtctct ccagaggacc ggattttcta ccgttccaaa ctggaaacca
     4141 tgaaatattt ttttcactat gaactgcccc ggacccaggc cctcttgcaa cggattggag
     4201 aagcggcccc catcacctta tttcccccgg atgaaatact gatctgatga ctgaagataa
     4261 aagctaccga aaaatcctgg tcgcccggga aggccccgtc ctgaccatca aaatcaaccg
     4321 gccggaaaaa agaaatgcca ttgacgatga actgttgctg gaaattgaac aggccttcct
     4381 gcaaaccgat ccatctgtaa aatgcatatt gctttgcggc gaaggggacc atttcagcgc
     4441 cggccttgac ctttccgccc tggaggagaa ggatgccgtt gccggcctgc gccattcact
     4501 catgtggcac cgggtcatgg atcatatccg gtttggcctg gccccggtga ttgcggtcct
     4561 tcgcggagcc tgcgtgggtg ggggcctcga attggctgca gcctgtcata tccgggtggc
     4621 agaagaatct gccttttatg ccttgcctga agggcagcgc ggcatcttca tgggaggggg
     4681 tggttctgtt catttaccgg gcctgatcgg cctggcggtc atgaccgaca tgatgttgac
     4741 cggacgtgtg atcccggcgg cagaaggatc aaggcttggt ttttcccaat acctggtgga
     4801 gaatggaaaa gggatggacc atgcccgggg acttgcggaa aagatctcgg ccaattcatc
     4861 cctcaccaat tatgccctca cccatgtttt accgaggatt gccgacagtc ccagggaaca
     4921 aggtctgatg atggaaagcc tgatggctgc catcgcgcag caatccccgg aggcaaagga
     4981 aagattgaag ttgttcttgg aaggaaaggc gccaaaactg ggcggcgcat gaccagaatt
     5041 gaatcccctg ttccttttcg tccggttgaa accatagctt atgacctccg gatggagcaa
     5101 aggccggatg gttcctttct ggttcaatcc aatataccgc ttgaagttca tccggtgcgc
     5161 atgactgagc ggttttgtca ctgggccgaa gtaagtccgg accgtatttt cctggcccaa
     5221 agggacgacc acggaacatg gcggacattg acctaccacg atgcctatgg ccagatgctt
     5281 ggtatcgcct cctggctgct gacccgggac ctgtccgaag gaaggcccct ggccatctgg
     5341 agcgaaaaca gcattgagca tgccctgctg gcccttgctg ccctgcatat aggcttgcct
     5401 tattccccca tctctcctgc ttatgccctc cggtcaaaag acttcaacaa actcaggcag
     5461 gtgatggatt tgctccggcc cggcatggtc ctggtctctg accccacacg ctatgacccg
     5521 gcccttaaag ccgcggccaa tggcttgaca gttcttgcct ccaaaacaag tccggatcac
     5581 aagggcctgt tgactttaga tcagatagcc aaaacgcctg tcgacccaaa agtggaatca
     5641 gcctaccaat ccatccggaa ggataccgtt gccaagatcc tgtttacttc aggttcgagc
     5701 ggaaatccaa aaggagtgat caatacccat ggaaacatca cagccaactg gcaacagatc
     5761 agccagactt ttcctttcct caaagaagaa ggcctggaat atgtggactg gcttccctgg
     5821 aaccatactt ttggcggcaa ccacaatttt ggcctggccc tgtatcatgg cgctacgctg
     5881 tacattgatg acggcaagcc cacacctgaa gggattggca ggacggtccg caacctgacc
     5941 ggaagaatgc ccacggtata ttttaatgtg cccaaaggat ttgaagaact gatcccttat
     6001 ttgaggaatg acgaaaccct tcgccggcaa tttttttcaa acctgaaaat gttgttttat
     6061 gccggagcgg gcatgcctca acacacctgg gatgctttgg aagaattatc gatcgatacc
     6121 attggtcaaa agatactgat cggcacaggg cttggctgca ccgagacctc cccctccgcc
     6181 ctgtttgcca gcaggcccgg aggcttcgct ggattattgg gggtgcccgt tccgggtctg
     6241 acactcaaac tggtccccac gggaggcaaa ttggaagcga gatacaaagg cccgaatgta
     6301 tttccggctt attggagcca agccgaactg acgcgggatg cttttgatga agaaggattc
     6361 taccggaccg gggatgcttt aaaatttttt atacccggag atccggaatc cggctttcta
     6421 tttgacggga ggctgacgga ggattttaag ctaagtactg gtacctgggt ccatgcagcc
     6481 cagctgaagg cccggattat tgccgccggc gccggcctga tccaacaggt cgtcctcacc
     6541 gggagcgacc gcccttatat cggtgccatt gtttttgtaa atcctgcaga ggttaaaaaa
     6601 atgctgaagg atgaattcga ccccttgaat tacactaaac acccatggct catcgccagg
     6661 atcaacagcc tcctttcagg attgaatagc gaaagcaccg gcagttcaaa ccggattgaa
     6721 aggatgatcc ttgccgatat ggacctgagc ctggacaggg gtgaaataac ggacaaagga
     6781 accatcaatc cggccatgat tatccggaat catccggagc ttgtggatca gatttatgga
     6841 taatatggag gtaatgggta aagaggtatg cgctctgccc ctttcccccg ctatcgctga
     6901 agatgaacct ttttgtctgg cattcctcgt tacttttaga gttcatcctg ctaaagccaa
     6961 tgcaagtatg ttatatttta attcaatagc ccaggagttt ggcgcccctt tacgggatgg
     7021 ggcaggggtt ttttaatgtt aaatattcac attatgagta ttcataatat atcggtggcc
     7081 cctgaagcca ccaatgcttg aagcttgcga caatttgtcc catcacggat attaaatgag
     7141 tagttgatcc gcgaagggga tcatttgtcc cgtgagggat gtaatgtggg tagctgattt
     7201 atcatggcaa atcccgtccc ggacgggacg gaatatatct ttaattcatt tctacccaca
     7261 taaagtgcct cccggcacaa acctccacca tctgcataaa gtttgtaatc taaccttgca
     7321 aaatttgggt tgcaccttct cgtttaatgg gtatccaatg gtctggaatt ccttgttact
     7381 tttagaggta gtttcaatta gttcgacatc aaaggcaatg caaggcgctt ataattaaaa
     7441 attcacataa tgaatattca taatatatca gccatcgtca ccgggggcgc ttccggcctc
     7501 ggagccgcca cggcccgatt gctggccaaa gccggggccc gcgtagccat ctgtgatctg
     7561 aatatgaccc tggcccttga cctggccaac cagatcaatg gcctcgccat ccactgcgac
     7621 gtggcggatg aaaccagtgc aagaggcgcg tttgaactgg tcaaatccac ccatggtccg
     7681 gcaaggatcc tggtcaattg cgccggcatc gccacagcag gccggatcgt tggcaaacag
     7741 ggtccttccc ccctacagga ttttgaaaaa accataagga tcaacctgat cggaaccttt
     7801 aatatgctga gactggctgc cgcggatatg atgacccttg aacccatgga agaaggagag
     7861 cggggggtga tcgtttccac ggccagtgta gccgcttatg aaggccagat cggccagtcg
     7921 gcttatgccg cttccaaggg cggggtggtc agcctgacct tgcctgccgc ccgcgaattt
     7981 gcccagtttg gcatccgggt caatgccatc gcgccgggat tgttcatgac ccccctgctg
     8041 gggggtttgc cggaagaagc tatccacaac ctttccgctt ccatcccctt tccgaaacgg
     8101 ctgggcctgg cggaagaatt cgctgccctg gtcaaacata ttatagaaaa ccgttatata
     8161 aatggggaga gcatccgcct cgacggggct ttgcggatgc aaccgaaata atttttccat
     8221 tcagcttttc aaaaaaatga tgaaaaacaa aatcaatcct cctttccgcg cggaccaggt
     8281 gggcagttta ttacgcccac cccatttgaa agaaagccgg ttgaaatgga aaagggggga
     8341 gatcacggcg gaagccctcc gggagattga agatgtggcc attcgcgaag tggtgaaaat
     8401 gcaggaatcc ataggcctgc attccatcac cgacggagag ttcaggaggg attattttca
     8461 ccttgatttt ttgaaagaat tgtcgggggt cagcgtcacc gggggcatcg aagccaatcc
     8521 ccatgccagg gtggcagagg atgggttcac accgcctaaa ctcagtgtca ccggcaaact
     8581 gaaacacgta aaggacatcc aggtggctga ttttaattat ctcaaatcgg tcaccagcca
     8641 gactcccaaa gtctgtatcc cttccccgac tatggttcat ttccgagggg gccggaaatc
     8701 catcgatatt gaggcctatc ccgatatgga tttgtttttt catgatctga gtgcctgcta
     8761 taaagcggaa atcgacgcac tataccgcaa cggttgccgc tatattcagt tcgacgatac
     8821 caacctcgcc tatctctgcg acccgaagat gagggccgcg gcggtggaaa ggggggatga
     8881 tcccaatgaa ttgccccgca cctatgccgg cctcatcaac agcgtgatcg atgaccggcc
     8941 ggaagacctg cgggtggcca tccatctttg ccggggaaat taccgcagca cctggtttgc
     9001 cgaagggggt tatgaaccgg tcgcagaaat cctgttcaac gccatccacg tcgatgccta
     9061 tttcctggaa tacgacgatg agcgcagcgg tgattttgcg ccgctccgct ttgtgccgga
     9121 ccataaaacg gtaatcctgg gcctggtttc ctccaaaata aataccctgg aggatccggt
     9181 agacctcatc cggcggattg acgaagcggc ccggtacctg cccctggaaa acctgggcct
     9241 cagccctcaa tgcggttttt ccagcaccca tcacggcaat gcgctcagcc aggacgaaca
     9301 atggagaaaa ctggagctcg tggtaaaaac ggcagcgcgc gtatggccag tataaaggac
     9361 taaaccaaga tctcgctttt atgaaatacc attttgtgct tgtggtttct ggtggtttct
     9421 ttaggcgtcc tcgcctaaag aagtacacct ttaaactgcg gatatttgat ccctgcctgc
     9481 ttcaggcggg gacgcctgaa gcaaccactt ccaaaataaa accttctcca attgaatgaa
     9541 tacaaattcc ctccacgcca tcgacttcca cacccacgcc gaggtcagct gccggcagcc
     9601 ccacgacggt taccggcctg aactggacga ggcttttacc agatacttca aatcggatca
     9661 ccgtccgacg atccaggaga ccgtggacta ctaccggtcc ctgaatatcg ccctggtcat
     9721 gtttgccatc gacgcggaat ttgaactcgg acgcaagcgg atacctaatg aagagattgc
     9781 cgaggctgcg gcggcaaatc ccgatatcct cattcccttt gccagcatcg acccgcacaa
     9841 gggaaagatg ggggcgaggg aggccaggcg gctgatcgaa gaattccagg tccggggatt
     9901 taaatttcac ccgaccgtgc agggctttta tcccaatgac cgcatggcct acactcttta
     9961 tgaagagatt gcggcggcag acctgcccat gctttttcat accgggcatt caggatttgg
    10021 cagtggggcc cgcgccggcg gcaaactccg gctcgaatat tccaatccca tccacctgga
    10081 tgacgtggcc attgatttcc cggatatccc catcatcctt gcacatccat cctggccctg
    10141 gcaggatgaa gccctgagcg tctgcctgca taaacccaat gtttacatcg acctcagcgg
    10201 ctggagcccc aaatattttc cgccccagtt gatccaatac gccaatactt tgttgaagga
    10261 ccggatgctc tttggcaccg atttcccttt gattaccccg gaacgttggt tgaaagattt
    10321 tgaaacggcg ggattccggg aggaggtgaa gcccttgatt ttaaaggaaa atgcgatgag
    10381 ggttttgggt ttataggtat tccaaatttt agaaatttgg aatacctacc gaattcttaa
    10441 tttaaacgag tggccacctt atcaataaat tcctttgcca tttccaatcc tcttttggca
    10501 attccggcat ccgtattgcc atcatagcct agactgaggt ggagggtatc gtacaggttg
    10561 ttaaactctg ccaacatttt tttgtcatat ttcgccaata gggaacgata ataatcaaca
    10621 gattttctga tatttttttg agttctcgac ttttcggagg gcataattag ctgatccaat
    10681 gcatggagca ctccacaata agcagtatgc cctgccattt ttacatattt tttatcttta
    10741 tagatgccat tcgttctttt ggctttttgt accagtattt ctttggcatt ggcaaggtgc
    10801 cggtttgctt cgcggatcac tggattcata atacaaaatt aatagaacga caaatacctt
    10861 ttccggcttt aacccacgaa ttggtaaaca cccggtcgtt cttcaatagg atgacccgaa
    10921 aagtcaggat ttgacctgtt aaaccgaaga tcggcttctg ttttcacccc tttttcctct
    10981 gcgccctacg cgcccccatc ggtttattaa aataaagaaa tcgttcattt aaccgccaag
    11041 atcgcgaagt gcgctgaggt tccgcaaaga catttcatta cccttattcc tctgcgcctc
    11101 ctctgcgacc tttgcgcact ctgcggttta tttaaatgga atattcttcc tttccaacca
    11161 aaagcatcga aggaagaaat cgttaattta accgccaagt tcgcgaagta cgccgaggtt
    11221 ccgcaaagac atttcattac cctttttcct ttgcgcctcc tctgcgacct ccgcgccctc
    11281 tgcggtttta tttaaatgca atattcttcc cttccaacca aaagcatcga aggaagaaat
    11341 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttatttaa atgcaatatt
    11461 cttcccttcc aaccaaaagc atcgaaggaa gaaatcgtta atttaaccgc caagttcgcg
    11521 agtaagccga agtaccgcaa tgacatttca ttaccctttt ctctctgcgc ctcctctgcg
    11581 tcctctgcgc cctctgcggt ttatttaaat gaaatattct tcctttccaa ccaaaagcat
    11641 cgaaggaaaa aattgttcat taaccgccaa gtacgccgag gctccgcaaa gacatttcat
    11701 tacccttttt cctttgcgcc tcatttgcga cctccgcgcc ctctgcggtt tatttaaatg
    11761 caatattctt cccttccaac caaaagaatc gaagaaagaa atcgttcatt taaccgccaa
    11821 gttcgcaaag tacgcagagg ttccgcaaag acttttcatt accctttttt ctctgcgcct
    11881 cctttgcgac ctttgcgccc tctgcggttt atttaaatga aatattcttc ctttccaacc
    11941 aaaagaatcg aagaaagaaa tcgttcattt aaccaccggg atcgcgaatt atgctgaggt
    12001 tcctcgaaga tatttccatc ccccttttgc tcttcgccct ctcagcacct tccttgcggt
    12061 ttatatcatt gcattacaaa gtcaaagaat cattacatta gaacaatgaa acaagtcctg
    12121 cagctcctcc cctttatcct ctccttgctt tcccttcagg gccatgccca gcatatccaa
    12181 ttattccgct acaacagtcc ggagggcgca aaaccagccg tccgcacctc cgagggtgcc
    12241 atgcttgacg tctccgggta caccacggat tttaatgaac gcttttttga agcaggcggc
    12301 atcccgggat tggtcaaatg gctggaaaaa aaccggaaaa aatgcaaacc cgtacccccc
    12361 tcctttacct atgccccctg tgtggcccgt ccatccaaaa tcgtcgccat cggattaaac
    12421 tatcttaaac atatccagga atccggagca tccgttcctt cagaacccgt catcttcttt
    12481 aaagccacca ccgccctgtg cggtcccaac gacccgctga ttattcccaa aaattcagtc
    12541 aaaacagact atgaggtcga actggccatt gtcatcggaa aaaaagcttc gtccatccat
    12601 gaagaagacg cccccggata tattgccggc tacaccatca tcaacgatta cagcgaaagg
    12661 gaatggcaat tggaaaaacc cggacttcag tgggacaagg gaaaaagcgc agaccatttt
    12721 gctcccctcg gcccatacct ggtgactcca gaggaagtag gcgaccccca taatctaaac
    12781 ctctggctca aagtcaacgg cgagatgcgc cagaactcca ataccggcga catggtctac
    12841 aaggtagccc ggctggtcag cgaagtaagt aaatacatga ctctccttcc cggggatgta
    12901 atcgctacag gtaccccttc cggagtcggc cagggtttta agccaccaaa attcctgaaa
    12961 cccggggatg tgatcgaact gggtattgat aaattggggg cgcaaaaaca gcttgcggtc
    13021 tcaaagtgaa cagcgatgtg aaatcatttt tccagatcaa aatcaattct aaaaccagct
    13081 atctgatcct tatgaaaata attctttctc ttttggccct ttgtattttg tttacgaatt
    13141 gcagccaaca acttccggtg acccatactg ttgactttga aacggaaaaa gcagccatac
    13201 aggccgtcat cgccaaggaa acggaatcct attataagca ggattatgat gcatggaaaa
    13261 gcacttactt acaatcgtcc gcttttcgca agttcggcta ttgggagggt taccctgaaa
    13321 aagtgacctc caatattgga tttgaatccc tggcagcgga aaagaaaaag cagttcgatg
    13381 ccaatgaaac gctttggcgg ggctcaaccg aggagcgctc aaacgaaaat tttcgcattt
    13441 cgaacgaaat ggcctggtat acttttgagc aaaactctta tgaaaaagac acccggaagt
    13501 tccttgggaa atcattagaa acccgcattc ttgaaaagga aaatggggaa tggaaaattg
    13561 cctttttggg gtttcattat tatccgatgg aggaggaaaa taaataaaga gatcacaata
    13621 cgtttttcta ccatcccgat tatagtagcc aatgacttcc aatttaaaat ggggattaac
    13681 ctgagaatgt tatatgaaac agaaagtgtc cttggtatta tccggaggag gggcaaaggg
    13741 gattgcccac attggggtca ttgaagaatt ggaaaaacag aattttaaaa ttgcgtcctt
    13801 aacaggaaca tctatgggag cctttgtcgg gggcatttat gctttgggga aaatggaaga
    13861 atttaaaaaa tggctgtttg ccctcgataa attgaaaatc tttaaactct ttgatttttc
    13921 gttcagttct cagggtttga taaagggcga taaagtaatt aacgaattaa agaaattcat
    13981 cccggatcaa aacattgaaa acttgaacct gctttacgcc gccgtggctg tggacctttt
    14041 aaacaaaaaa gaagtcgtac ttgacaaggg aagtatctat gaagccatgc gggcctctat
    14101 tgccatcccc accgtcttta cgccggtaaa aaaagacaat atgctgctgg tggatggagg
    14161 ggtattaaat aatatcccca tcaatcacgc taaaagaagt ccgggtgatc tcttgattgc
    14221 cgtgaatgtt aacgccgata ttccccttta tcatccctcc ttattaaagg aagaatttga
    14281 aaccaaacaa tccatctaca gaaaaaaaat cagggaattt caaattcaat tgcaaaagat
    14341 cattcctcca aataaggatg aaagccttga ttatttcgat ctgatcaata aaaccatcca
    14401 cttaatgatt gagcgcatta ccctgaccac cattgaaaaa tattcaccgg atattctaat
    14461 caatatttca agaaactcat gcggaacttt tgatttttat aaagccgagg acatgtacga
    14521 gatcggttgt tattcagcga aaaaatacct ggaggagtat aaaaaaagtt atgagcaaag
    14581 gagaatatcc gagacaaaat aaatactcat cgtagtaggt attttgacat tgattgagct
    14641 tttattaagt ttatccttac tcaaaaccgt gaccgccagg aaaaacattt ttgttatcat
    14701 cggaagtgca agtaaaaatt ccgcaaacga aaaaatcatc gattatatgg tcaacttaac
    14761 ccttgacttt tttagcatga ccgtttttaa ggatttaaag gcactacccc ctttcgaccc
    14821 cgaacaaccg ccactggatc aattcgtaca ctcatacaaa cggttggtta cccaagcgat
    14881 gagtaactta acaatgacca tttaatctta agagctttgg ctgaatgtat ttcctttatg
    14941 gcttacaggt tgcttttgga gcgagccggc aaaaaatgac gggttaataa aacactgaaa
    15001 tttcgaatgt atgattggca tactcattca actggctgtt tcatggatac tgctatggtt
    15061 gatggacaaa aaagacctca ccgttcttgg attaaaaccg accaccgacc gacttcggaa
    15121 attctcattt ggttttataa tatctgccgc ctggtttacc gtttattgcc agacgaccac
    15181 cttcctgacc aataatcatt ggacgataaa tcaggatttt tcactaaaag aatttttatc
    15241 ctcaacctgg tggaccttta aatcagtgat ttatgaagaa ctgatcttca gaggggctat
    15301 actctttctt ttgattcaaa agactaatat aaaggcaggg tgtatcatct cctctgtagc
    15361 gtttgggatc tatcattggt tttcatttgg agtgctgggc aaccctgtac aaatgactta
    15421 tattttcctt tggaccggta tctggggact gatgtttgct ttcgcttttg caaagacccg
    15481 gtctttatac ttacctacgg ggcttcattt gggatggaac ttactgaaca tagtcgtttt
    15541 ctcacaaggg ccgttgggaa atcaattttt aattcacagc aataacggac aaggtttgac
    15601 cgtgttccca tctgtcgggc ttcaaatttt tcaattgctg ggacttccaa tcatggttta
    15661 cttatattta aaaaattaca ggcaatcaga ccacaagtag aaattgttat actttcttac
    15721 tttcattccc catattcgac cctcatgcag caaaataatt tcgatgccat cgtcatcggc
    15781 tccgggatca gcggaggctg ggccgccaag gaattgtgcg aaaaaggact gaaaaccctg
    15841 gtgctcgaaa aaggcagacc ggtagtccat gtcagggact atcccacggc caacctggat
    15901 gcctgggata tggaattccg gggagccctg acccgggaag aagccaaacg atttcccgtg
    15961 cagacccgga gcggtttcgt gggcggggac aataaacatt tttttaccga cgacctggac
    16021 aacccttata tcgaaacaca acgttttgac tggatccggg gcaatcaaac cggggggcgc
    16081 tccctcacct ggggccgcaa ctgctaccgc tggagcgacc tggatttcga agccaataaa
    16141 aaagacggga tagccataga ctggcccatc cgctaccggg atatcgaacc ctggtatact
    16201 tatgtggaaa aatttgccgg aatcagcggg gaaaaactgg ggctgtccca tttgcccgat
    16261 gggtatttcc tgcccccgat cccactcaat tgcatcgaac aacacctgaa aaaagaagta
    16321 gaaggaaaat tcaaaggcag gcatatcacc cctgcccggg ttgcccatct gaccgatccc
    16381 cagccctggc acctggaact gggccgcggc aaatgcatga accgcgaccg gtgtgccagg
    16441 ggttgcccgt tcggggccta tttcagcagc aattcggcca ccctccccgc tgccgcacgt
    16501 accggcaacc tcacgctccg gaatcatgcc gtagtgcatt cgatactcta tgacgagcag
    16561 agcggccggg ccaccggggt ccgggtgatc gacgcggaaa ccatgatcag tacggaatac
    16621 cgggcttccc tggtcttttg ctgcgcttcg accctggcta cgacccagat cctgatgaat
    16681 tccttatcct cccgattccc aaacggaatg ggcaacgaca gcggtcaact gggccgtaat
    16741 ctcatggacc accactaccg ctgcggcgct tatggcgaac acgagggtta tgcggaccaa
    16801 tattataagg gcaggaaacc caccggcctt tttatcccac gttatgtcaa cctggacaga
    16861 ctcacggaga ataaaaattt tatccggggc tatgattacc agggcgccgg cggtacccgg
    16921 aacccctgga gccgaggaat ccaggaaccc ggcgtgggcg cagaattcaa ggaatccctt
    16981 ttcaagcccg gaggctggaa tatgggcctg atgggttttg gcgaatgcct gccttatgag
    17041 gacaaccgac taagcctgga ccccgagaaa aaagacaaat ggggcctgcc cctcctggtc
    17101 atcgatgccg gcttcaggga aaatgaaatg aagatgcgcg aacaaataaa agccgatgcc
    17161 gcagagatgc tcgaggtggc cggttttaaa aacgtccggg tatttgatga aatcgggggc
    17221 atgggtgtag gggtccatga aatgggcacc gcgcgcatgg gacgggatcc caaaacctcg
    17281 gtcctgaacg ggaacaacca ggtccacggc gtacccaatg tatttgtgac cgacggggct
    17341 tgcatgactt cggcttcctg cgtcaatcca tccattacct atatggcgct cacggcaaga
    17401 gctgttgatt ttgccctcca ggcgctgaat aaacgcaacc tttaaatcct tttccatgaa
    17461 ccgaagagaa gcgatcaaaa atcaaagcct ggtcattttc ggagtggccg tatcggccag
    17521 cgcctggtcc ggcttatacc tatcctgccg gtcaatgccc gttctagact ggaaacccgg
    17581 tttttttacg gtggaggagg cctccctctt aaccgctatc acggaaacca ttctgcccaa
    17641 aacccagaca gccggcgcga tggaaacggg cgtgccccgg tttattgata aggctatcgc
    17701 ggccttattc gatccggaaa cccaacaaaa aatccgggac ggcctggacc agatagaccg
    17761 catggcgaaa accactttcg gcaaaaaatt tccctcttgc agcgaggaag aaaggacttc
    17821 gctgttgttg gacctggacc ggaaagcggg aacttatccc ctgtctttgt ggggcatcac
    17881 cctggaacaa tccaagcctg ttggattctt tcgtgattta aaaagcctga ccctgatggc
    17941 ttattttacc tcggaggaaa tcgggaaagg ggtgttgagg tatgatccgg tgcccggtga
    18001 ttaccaggct tgtttacccc tcgatgggat gaacagttgg actgaataat gcctgactgc
    18061 agttcttaat tattttaatt tccttaattt cttaattcta catgaaaggc ttctccatca
    18121 tcatttgttt ctttttattg agtttgaata tatcctcaca acaagtcatc ccattatact
    18181 ccggttctgc tcccggatcc gaatcctgga cccaccagga aaaaacagat accctccggg
    18241 gcccgaataa ctacattttc gtctccaacg tagtaaatcc cagccttatc gtgtacaggc
    18301 cggaaaaacc caacggcatg gccatgatcg tctgccccgg gggagcattc agggccctct
    18361 ccattcaatc cgaaggcacc gatgtggcgc gatggctcaa taccaaagga atcaccgctt
    18421 ttgtgttgaa ataccgcctg gtaccggggc atattgcttc cagggaacaa gccatggagg
    18481 atatcaggac tcaaaattcc aggaggatcg attctctgaa tgctccctac gtaccgcttg
    18541 ccgtggcgga cggccgcgag gcggtaaaat acgtccggac ccatgcggcc gaatgggggc
    18601 tgcatcccaa ccggatcgga atcatgggtt tttcggcagg gggtacggtg gcggcttccg
    18661 ttgccctcac ccctcaatcc tcaatcctca cccctcaatc ctcacccctc acccctcaat
    18721 ctatgttccg tcctgatttt gcagcaccta tatacgccta ttgtggcgcc attgaaggaa
    18781 accaggtacc ggctgatgct ccacccctct ttctcgcttt ttcacaggat gattttatat
    18841 cggatggaaa tccggcctta taccaaaaat ggaaagctgc cggcaggcca gctgaaatgc
    18901 atgtttatcc ggatggcggg cacgggtatg gcatccggaa acaaggcaag gcctccgacg
    18961 gctgggaaaa ccgcctggcc gaatggttga attaccagtt ccccgttcag gaatttggtt
    19021 cgttctggaa agacctcgat tatgttgggg atcaaatccc ggggcataaa ctggatatcc
    19081 atttgccatt taataaaaaa ggccccttcc ctgtgatggt tgcgatttat ggaagcgcct
    19141 ggttttccaa caacgccaaa caaaatgtat ttcgtgaaaa cctgggccgg cgattgctgg
    19201 aagcagggta tgccgtggtc agcatcaacc accgttcgag ccgcgacgcc ctcttccccg
    19261 cccagatcca ggatgtcaaa gcggccatac ggtttgtccg ggctcatgcg ggaaaatacc
    19321 aactggacac ttcttttatc gggatcacgg gctggtcctc gggcggccat ctttccgcat
    19381 tggcgggagt gaccggtgat atcaggcggt ataccttagc cggtgggacc atggacctcg
    19441 atggttccat tgggccccat accggtatga gctgcgcggt ggatgcagtg gtcgactggt
    19501 ttggccctgt ggatttcctc atcatggatt cctgcggaag ttccttcagt cacaacggcc
    19561 cttattcgcc cgaatctttc ctcatgggaa gccctatcca ggaaatcaaa actaaagtcg
    19621 cctttaccaa ccccgtccat tatatccacc cgggcacacc gccgtttctt atctttcatg
    19681 gggatcagga tttccaggtg cctgcttgtc agagccaaaa attgcatgag catcttataa
    19741 tgaaaaatat ccaaagcgaa tccatagtcg ttccgggagg aggacatggg ccaggggtga
    19801 tgatcgatta ttattacgat aagatgatcc ggtttctgga tggggagttg aaaaagggga
    19861 aataaagtca agcgttggaa ctcatttctc tgcggttata ttgtgctccc ggcgggctct
    19921 gcggtttcaa gttttccgaa ccactgattt acagatcact aattaccgac tgattcctga
    19981 ccattgatta ctgattactt cttcatccgc acacccctga cccctgaaag gggactgccg
    20041 cacagattaa gaagcgagca acgagatgcg agaagcgaga taaaccaata tgttaacttg
    20101 ctattgccta ctgactattg actactgact attgactatt tccttaggtt tgcaatccat
    20161 atgaaaaaca aattaatcct cctcatagcc atatataccc tgacttccct gattctccaa
    20221 agccaggatg tattcaaaac ccaaaggacc gcctggctcc aaaaagccga agccctcaaa
    20281 cccaaactgg cccaggtcaa agtcaaaccc gttcatgcga tcaccacgag gcctgaccgg
    20341 ggtgccttcc agggatggag ggtggaccgg aaatggtcta ccgacagttt gtttacgaag
    20401 tcgtttaaaa accagaccgg actgatcctt gatttcggac aacacctcac cgggtatttc
    20461 accatgaaac tcgaggagat ccgcggagtg gcggacgccc ccacccggat caaacttaca
    20521 ttcggagaag tcccctcgga agtcagtgtc ccttttgaac cttataatgg cgatctgagc
    20581 agggcctggc tccaggacga gatcgttacc gtctccgaaa tgccttcgga gttcacctta
    20641 accaggcggg tttccttccg atatgtaaaa ctggaattgc tgggcgcttc ccggtttttt
    20701 gattttaaga tcgccgatgc ctggtgtatg gccggcacct ccgcggcggc cgttcctcct
    20761 ccgctgaaac ccaccaccta tgaagccata gccaaaatcg attcgatcgc ccgcaatacc
    20821 ctgaaggaat gtatgcagac ggtatttgaa gacgggccca aacgggaccg ccggctctgg
    20881 atcggcgacc tctacctgca gtccatcacc aacaatcata cctttaaaaa ccacaacctg
    20941 accaaacggt gtctctatct gcttgccgga ctgagtacgg aagagggtta cctcccttcc
    21001 aacgtctttg aaaggcccgg accccattcc cagaaaggag cgcccttttt atttgaatat
    21061 tccctgcttt acaatgccac cctgctcgaa tacctcaaag ccaccaacga ccggactaca
    21121 gccctcgacc tctggccggt tgccaaaaaa caactcgata accctaaaaa atttatgctc
    21181 gacaacgggc tcttcaacga cacggccgcg gctgccaacc gttggtggct cttcatcgac
    21241 tggaaagaag gcctggacaa acaagccccg atccagggcc tgatcatttt cgccttgaat
    21301 caaaccgtcg aacttgccaa aatgctgggc aaacaaaacg aagtcgcgga aatccccgcc
    21361 ctggttgaaa aaatgaagaa agcctgcatg gaccatcttt atgataagga cctggggctc
    21421 tttgtcagcg gccccggcaa gcaggtttca tttgcctctc aggcctggat ggtattggcc
    21481 ggggtaacgg acaccgcgga aagtcagaag attcttacct atgtcaaaga acacccgact
    21541 gctgtcaaac ccggcgggcc ttacctcatg cactattatg tcgaagcgct catgcgctca
    21601 ggcctgacca gcgccgcccg ccagcagatc ctgtcctact ggggcaatat ggtgatcaaa
    21661 ggggccgaca ccttctggga agtatatgat cccgaaaacg attatgcgtc gccctataaa
    21721 ttttacccgg tcaacagtta ttgccatgcc tggagctgca cgccggctta ttttataagg
    21781 aaatatcccg aagtgtttca acagtaagag cacacagggc gaaaaaagac acggaggctt
    21841 ttggtattgg ggcacttcat gcggacgggg acatcatttt agttcaaagg atctgggcta
    21901 aagcccctgg ttcgatagga actcataaac cccagcctga aggcnnnnnn nnnnnnnnnn
    21961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnngatctg ggctaaagcc cctggttcga taggaactca
    22081 taaaccccag cctgaaggcc ggggtttgaa tgcagcggtt gaaagcgcac ggagcagagg
    22141 aggacacgga ggctttaggt attgggcact ttatgcggac gggagatcat tttagttcaa
    22201 aggatctggg ctaaagcccc tggttcgata ggaactcata aaccccagcc tgaaggccgg
    22261 ggtttgaatg cagcggttga aagcgcacgg agcagaggag gacacggagg ctttaggtat
    22321 tgggcacttt atgcggacgg gagatcattt tagttcaaag gatctgggct aaagcccctg
    22381 gttcgatagg aactcataaa ccccagcctg aaggccgggg tttgtatgcc ctatccataa
    22441 ctgaagaagt atcgcctatg ccaggcttca ggtagaggcg cgactcggtg aaaaattgtc
    22501 aaacctcagg cttcagcctg gggttcagcg caaaagagga taatagggct ttagccccgg
    22561 ataatatgac ctaaagggat gactaccagt caaccttaac ttcgacaccc ggtctttctt
    22621 cttttattag atttgcaaac atgaaatttc taagcatcga agatatctcc aaagcaaatg
    22681 aacgagtcgc ctccctggtc cataagacac ccatctttac ttcgtcctta ttaaaccaat
    22741 ggatgggcca tgaactatac ttcaaagccg agtgtatgca aaaaatcgga gccttcaaag
    22801 cccggggagc atgcaatacg gtagcctggc tcctggaaaa taatgagaaa cccaaacaca
    22861 tcgttgccaa cagttccggc aatcatgccc aggccgtggc ctgggccgca agccggtttg
    22921 gtatcccttc gaccattttt atgcccgcct attcctcaaa ggtcaaaatc caggcaacca
    22981 aatcttatgg cgctgaggtg attttgtgtg agacccgggc ggtggtggat gaaaaagtta
    23041 agctggcttc agaggaagaa gggacctgct ggatccctcc ctttaaccac gaccaggtca
    23101 tcgccggcca gggcacggcc gcttatgaag cgatagaaga agtgggggaa gtcgatggcg
    23161 tttttgcgcc ttgtggagga ggcggcttgc tttcgggcac ctgggtggct tcccgcggac
    23221 tggccccgaa ggccagcgtc atcggggcag aacccttgaa tgccaacgat gcggcccaat
    23281 ccctgcgcat gaattccatc cagaaattgc aatccgtacc cgataccctg gcggacgggg
    23341 ccatgaccat agccgttggg gatctcacct ttgaatatct gaagcagctg gacgattttt
    23401 atgaaattga agaatcccgg atccggtact ggacccagtg gctgacccat ttattaaaag
    23461 tccgagtgga acctaccagc gccatggcca tggaagccgc tttccgatgg ttgtccaaac
    23521 aaacttcaaa aaaaagggct ttggtaatcc tgtcgggtgg aaatattgac caggaaacat
    23581 ccttaaaaat atggaaggac aattgcctgg atgaggtgcc gcgtttataa ggatccgccg
    23641 gagtaactgc attttgtcat acccctgaaa ttgctaatca cggcagttcc gtttaagatc
    23701 cataccttta acacctataa cattccggcc gggcaatgtt cagtcccctg caattatcca
    23761 ttcgtttcca gtacctcgtc agtattctcc tcattttcct ggtatccggg gtcagttatt
    23821 atctaaccga ttataccggg taccgggtgg ttgccctggt ccttttgatg accgtttctg
    23881 ttctggccat gcttttccgg atcttaccgg ttctgctcgc ggccctgttg agcgccctca
    23941 tctggaatta tttttttata ccgccccggt ttacctttca catcgatcac accgaagacg
    24001 ccctgatgtt tttactgtat tttctcatcg ccctgatcaa tgcggtattg accatcaaga
    24061 tcaggcaagc ggaaagccgg gccagggaca aggaagaaaa agaaacaacc atcaggctct
    24121 acaataccct gctcaattcc ctctcccacg aactgcgcac ccccatcgcc accatcctcg
    24181 gctcagtgga taccctgaaa gaaaaccgtg ataaactttc cgaaaaaaac caggccgaat
    24241 tgcttacgga aatcgacaca gccggcattc gcctgaaccg ccaggtggaa aacctcctca
    24301 acatgagccg cctggaaacc ggcctgctca agcccaagcc cgaatggtgc gatctgaatg
    24361 aactggttca ccgggtcatc cggaaatatg aaaacccaga aacctgcacc ctccgcttcg
    24421 aacccgacga gatgctccca ttgcttaaaa tagatggagg cctgctggag accgtgctcg
    24481 acaatttggt gcagaatgcc atacaatacc atcccggcca cgccctcgta gacctttccg
    24541 tccggtatga gtccaacacc tgtctgatca cggtttcgga ccatggcgcg ggattcccgg
    24601 aggaagaaat cccaagggtt ttcgacaaat tctaccgctt gccggacagc aaaacgggag
    24661 gcagcggctt gggtctatcc attgtcaagg gttttgttga agcgctgtcc ggaaaagtaa
    24721 gcctggaaaa taacaaggaa gggggtgcaa aattcagctt atccataccc gccgaagcct
    24781 catacctgaa gaacctgaaa aatgaataag gaggaaatac tgatcattga ggacgaagcc
    24841 cagatccgca aactgctgga gatcctcctg gaaagcaacg attataaagt ctggcaggcc
    24901 ctatccggaa aggagggcat tgtcctcgca gccaatcatc ccccggacct gatcctcctt
    24961 gatattggtt tgcccgaccg gagcggccat gaaattctaa aagagctgcg aacctggtac
    25021 aaccggccca ttctgatgct ttcggtccag gacaaggaag aagacatagt ttccgcgctc
    25081 gaccacggtg cgaccgatta catcaccaag cctttccggg cggcggaatt attggcccgt
    25141 atccgctcgg ccctcagaag aagccagccc ggtccggcaa cggcatgcat cacctgcgac
    25201 gacttgcaaa tagaccttgc cgcaaggacc gtcaaaaaaa acaatgaacc ggtgcggctc
    25261 acgtccaccg aatttaacct gctcgcctta tttgtcaaaa acgaagggcg ggtattgacc
    25321 catcattatc tcctcaagga aatctggggc cccgggtttc agacggaaac tcaatacctc
    25381 agggttttcg tgggaagcct tcgtaaaaaa atcgaggaca atcccaatca gcccaggcac
    25441 ctgatcaccg agagccgggt gggctaccgg tttatttgag tcccctggtt tgatccggcg
    25501 atttccatgg atggggtcag cgtctgagct ttctgtttgt ctttataatt tctttatatc
    25561 cgcccggtac agttttactt tttaccctta tccgccggcc cacctttgca ccttattgat
    25621 ttaagatgga aaagccaaaa aacgggagtt atcacaaagt gaccgcggcc acccttttgg
    25681 tcgccctggg cattatctac ggagatatcg ggaccagtcc actttatgtt ttaaaagcca
    25741 ttgccggtga ccggccggtt tcggaaaccc tgatttacgg aggcatctcc tgcgttttct
    25801 ggaccattag tttcctgacg acctttaaat acatctacct cactttaaaa gccgacaacc
    25861 atggcgaagg ggggatcttt tctctttccg ccctggtccg cagatatggc aaatacctgg
    25921 ccattcccac cattctaggg gcaacgaccc ttttggccga tgggatcatc acccctccca
    25981 tttccgtggc atccgcggtc gaaggcctga ccgggctcaa agggcttgaa aacctgccta
    26041 ccgtcccgat cgtgattgtc attttatcgg ccctgttttt cttccaaagg tttggcacgc
    26101 ataaagtcgg ttccaccttt gggccggcca tgctggtatg gtttaccatg ctttctaccc
    26161 tcgggatgat ccagatattc cggcatcccg gcatcctgaa gtccctgagt ccggtatatg
    26221 ccttccggtt cctgactgaa tatccgcatg gtttctggct cctcggcgcc gtcttcctgg
    26281 ccacgaccgg ggcggaagct ttatattcgg acctggggca ttgcgggagg aaaaacatcc
    26341 gcatcacctg ggtgtttgtc aaaacctgcc tgatgatcaa ctatatgggc caggccgcgt
    26401 ggatcatgaa cttgggggaa ggacaattcc tcgacggcag gaatcccttt ttcgaaatca
    26461 tgcccgagtg gtttttgatt cccggcatcg tcatcgccac cctggcggcc atcattgctt
    26521 cccaggcgat gatcagcggc agttatacct tgatcagcga ggccatgaac ctgaatttct
    26581 ggcccaggat cgcggtcagg caaccgactg aactcaaggg acagatttat atcccgagcg
    26641 tcaatggcat tctttggatg gggtgcatcc tgatgatcct ttatttccgc tcctctaccc
    26701 atatggaggc tgcatacggg ttttccatat ccatcaccat gctcatgacc accttcctga
    26761 tgatccactt tctgcggtat aagctgaagt ggaataaatg gtttgtcttc gcggtcttcc
    26821 tgttgtttgc cgctatcgaa tcttcctttt tcatcgccaa tgcggccaaa ataaaagaac
    26881 gctggatgtt tctcttcttc gaacttttta tattcctggt catgtatata tggtattacg
    26941 cccggaaggt caacaaccgc tttacccgtt tcgtggaact aggccagcac gtccccgcca
    27001 tccgcgagct cagccgggat gaagacatcc cactattttc aacccatctg gtatacctga
    27061 ccaaggccaa caaccggcat gagatcgaag aaaaaatcat aaaatccatc tttgcacgca
    27121 aacccaaacg ggcggacgtc tattggtttg ttcatattca ccggaccgaa gagccttata
    27181 cccttaccta cagcgtaacc gaacttgtgg atgaccaggt gatcaaaata aatgtaaacg
    27241 tgggctttcg cattcaaccc cgtaccgaac tctattttaa aaaaatcatc caggacctgg
    27301 tggccaacca ggaactgaat ctccaccaga ggccggacgg atccaacaag tacaaccacg
    27361 aaccctcctt taaatttgtg gtcatcgaaa aatacctctc ggttgaaaac gaattttcgt
    27421 tccgcgaagg cttgctcctg aagacctatt ttcttttaaa gcgggttagt ttaagtgacg
    27481 aaaaagcctt tggcctggat aaaagcgatg tgctggtcga acaggttcct ttgatttatc
    27541 agccggccac gaggtttgaa ctgaagagag agcccgccaa accgatccaa gcctgatccc
    27601 ccggcggccc ataaattaat taagccattt aaatttctta aacggtattc cctttacata
    27661 atttttcaca agcatatcat tcggtccgct ttaaaaccct gaatgaacaa gtaataaatg
    27721 gcctttccgt gaaatttccg ggtttcattt cgcttggata aaatacaaag aacgcaagcc
    27781 gtcataaaac caaggattgc caaggcacgc ggttattcaa tgggagaaat ttcagtctca
    27841 cataaaccag ggtcctaagc tttcaacact tctccaggat ctttttagcc aactttatcg
    27901 ccaattcctg gttgtcttcc acattcccac tgatctccac gaatacctta aaccggaccc
    27961 ggccgacgag gatgaccagg gaatggtcca tatggtccca ggctgccgca tcgccgagac
    28021 cctctacgtt cgtgaacaaa atctgctcag acaaggcagc agtcagcgtg gtacccgccc
    28081 ctttctggga actggtcaat cctttctcct ccattttcgt tttcatcctt tgctgcaatt
    28141 ctgatttttc ttctgccgtc ggcgtccggt gtgcattccg gaaataggtc aagggatctt
    28201 taacccggtc tccgtaaacg gttaagtgtc cgacccctat tttattgggt cttggtgagc
    28261 gaacttcccg ggagcctacg aatgaaatcg aaaacctgtc gcttttccat tgataaacgc
    28321 tggatcgata agcgattggc acgttccgcg aataattgat tgccgcttcg gccggcaggc
    28381 cgggaaagat ggaaacaata tcttcccggg tcagccattc atccagccgg tcagcgtatt
    28441 tttcaaaaca ggagggaggg gcagtaggct taaaacagga agaagcgtca tgggaaccgg
    28501 gtatagcagg tttacccagc agcatgcacc agccgataaa agaaatcaga atcatgatta
    28561 tccgtattca gtccgacagc cggaagatgg tccggtccgg gctgcctttt ttactcagca
    28621 gggaatatcc tgttttttct gttcggcctg ggcatagacc ggtctccatt gctctttggt
    28681 ggactcatcc aggcaattga tccacaatgt aaacttatcc agatattcta aataggcttt
    28741 cttgtatgcc atgcaattct cagcagagtc atcctgcgca tgggcaattt gcgcactgat
    28801 agaaaggttc tgggcatcca gcattgcctg ctccttttga gaaagttcag aagcgctgca
    28861 atttgaatcg tctgtacttt ggctgcagga actgattgta ataaaaagca agccactgaa
    28921 tatcagttgt ggaaatctca tgtttaaaaa atttaggttt ataaataaat taaataaact
    28981 gcatgttctt tcgccgatat cgtatttcaa acatacccct tttaccggta tcatgccatc
    29041 agatccactt gtttaacata tttcactaat ttagtagaac catgttaccc agagaaagag
    29101 tttggaatac atccatcatg aaaaccgtat gcatcatttt ttttcatttg tttttgacct
    29161 ggtctgtcct tggccagatg gttctgccct tgtaccccgg aaacataccc aataacaagg
    29221 aagtctccgg gatcagcgat accgcgaata cctttatggt tgatcatacc gtgaagcggt
    29281 tcatcacccg gatcaccaaa ccggagttga ctctttacct tccggataaa aagaaaaaca
    29341 gcggttatgc ggtcctcatc tgccccggcg gcgggtatca tgggctggcc attgaccatg
    29401 aaggtcatga catggcaaaa acactggtgg aagcaggaat cgcagggatc gtgctgaaat
    29461 accgatgtcc caaacctgaa tatgtggtaa ataaagagat cgttccgctc caggatgccc
    29521 agcaggcgat gctcttgcta aggacccgcg cgaaagaatg gggcatccgg gaaaacagga
    29581 tcggcatcct gggttcttcg gcgggcggcc acctggcttc cacggcgggg acccatttcc
    29641 tggaccaaaa gattgaaaac ctaaaagggt tgagtctgag gcccgatttt atgatcctca
    29701 actatcccgt gatcagtttt gccgacagcg tcacccaccg cggatcccgg aataatttgg
    29761 tgggcacccc gccttacgat tcctttgatc ctgaaaaaat cgcctactac tccaacgaac
    29821 tccaggtcaa tcccactact ccgagaacct tcatcagtca cgcggaagac gacaaaacgg
    29881 tccccatagc caatacccgg ctttttatct cagcccttga aaaaaaccac attcccgtga
    29941 ccgctgtcat ttatgaaaaa gggggtcatg gatttggaat gattaatccg acgagcgata
    30001 gagactggat gaaggaatgc attcagtgga ttaaaaaggg cggtaaagag aaaaataaaa
    30061 aatattgcag tatgtaatcc ctgatttagc gaccggaatg aaaagtatta tagattacat
    30121 ccctatcgtc accacattct tctccattta ttttactgtg gttatttaca ggcattacca
    30181 actgcggaga acaccttacc tgctgtggtg gaccattggc gtactcactt ttggtctcgg
    30241 cacgctggcc gaaagcatcc atgcccttgc aggctggaat gagataaact taaaatattg
    30301 gtatatcatt ggggctctgt tgggtggttt tccacttgcg caaggcaccg tttatttgct
    30361 catgccccgc tggtttgcca ggatcagttg ttatttcttc gtgacgctta tcctggttgc
    30421 ttcggtctgc gtcatgctca gcccaattac tataccggag gattttaacg gcaaactgac
    30481 cggggcggtt ttttcctgga aatgggtcag atccttttct cccttcatca atatgtatgc
    30541 ctttttcttc ctcgtaggcg gagccttata ctctgctttt aaatatttca cttccggcga
    30601 acgcaaagct cttttcaaag gcaatatcct catcgccctt ggagggcttc ttccgggaat
    30661 aggcggcacc ttcacccgga tgggttacgt ggaagtcttg ttcgtcactg agctgatcgg
    30721 cttgtattta atttacctgg gctaccgaac gataaaaaaa gaaaaacaac tcctgcaaaa
    30781 cagcgccacg gacaagggat tgtttcttgg gtcctagcct tgaagcggtc aggactcaaa
    30841 aaaatcaata aagatttgta tccattttag gtgaaccgat tgcgaacctc cgaaatgcat
    30901 acccctaaag catccttttc aatgattgac ttatggcata cgatcaaaaa cttgctcacc
    30961 ggatccgcca ccggctgtcc ggccttccca atgtcgaaga gaaagaaatg atgggcggtc
    31021 ttacttttat ggttaacggc aagatgtgtg caggaatcat caaggatgca ctgatgtgcc
    31081 ggatcgaccc ggaattccat gacctggccc ttgaaaaaca gggttgccgg accatggatt
    31141 ttacccatcg tcccatgcgc ggttatgtcc tggtggatga atccggcatg cactccgata
    31201 aggactttga ttactggata aaccttgcgc ttgaattcaa taaaaaagca aaagcctccc
    31261 gtaaaaagaa aaaatagaga aaaaccagcc attcgggagc gggagggagt aaatagaaag
    31321 gagaattccg atctttggaa gaaaccggat taattatgaa taatgagcgg accccgcata
    31381 tcctcaatac ttcagctacc ttgttggggc tttgttttat tgtgctcacc tcccggaatg
    31441 tattgcctga ggcggaaatt acggtaatcg attatcttac tggcctggcg atcctgggtt
    31501 ttatggccgc ctgtttgctt tcctatatgt ccatgcgcaa gggggaaaaa tcaaaatggc
    31561 cactggaaac cgcagcggat tctgtcttta ttgcaggttt gttttttctg tttgccgcca
    31621 ctttattgat cgttttgaat attatttgaa aaggagtccg cactccgtat ttaccttttt
    31681 gcctgattaa ctgatacctg attactgatt acagagtctc tggaatatcc cttcctccct
    31741 ttgttccctt tctccctttc tcctttgttc ctactccaaa tacccaaact tcccctttct
    31801 gaaatcatca aaagcctgag cgatctcctc cctcgtattc atcacaaaag ggccgtgggc
    31861 cgcaatgggt tcttcaagag gttggccgct caggaccagg accacagatg gttccaatgc
    31921 ctggatgctg aaggaatccc catccgtccc aaaaagcaca aaatgattgg cgggcacttc
    31981 ttctttttca ttgacttgca cctttccctc aatcaccagt agggccgtgg caaaccaggc
    32041 gggaaaactg aaatcggctt cggcgccctg attcagacgg gcattcatca ggtgcacggg
    32101 tgagaaggtg gttgccgggc cctgggtttc ctcatatttt ccggcaatca cctcgaccat
    32161 gccctgccct tcggacagga tgtgtttgcc caattgatgg tggctcaatg cctggtactt
    32221 cggaggcgac attttaaatt cagccgggag gttgacccag agctgcacca tctggaagtc
    32281 tcccccggac ctgctgaatt cctcttcatg atattcctta tgaaggatcc cggaagcggc
    32341 ggtcatccat tgcacatcgc cttcccggat caccccgctg ttgccggcac tgtcatgatg
    32401 ggccaccctt cctttgtaag cgatggtcac cgtttcaaag ccccggtgcg gatggacgct
    32461 gacccccctc ggtttatctg aaggggggaa ataatgcggc gatccatagt ccagcataat
    32521 gaagggatcc atgcgttcca tatccagccg gtaaccgctg ggtataaaat tatgtacgcg
    32581 gaaaccatcg cctacataat ggggttcccg cggagggacc acgatttcga ttgatctttc
    32641 agccatatct gagggattga attattcaaa acaaaggtag acaggaaatg ttcagcaaaa
    32701 agtgagctaa agtgaagggt tgactttagt gaagggttga ctgttagtca aaccttcact
    32761 atttcactat attaccatcg ttttgaaaac ttccgtcgcc attatcgggg caggtcccgc
    32821 cggccttagc ctcgccttac tgcttcatca ggccggtatc ccttgcctca ttctggaaag
    32881 acattcccgg gcctatgtgg agtcccgcgt aagggcggga ttgctggagc agaatacggt
    32941 ggaccttttc acggaactgg gcgtcgccgg ccggcttcgc cgggaaggcc tcgaacactt
    33001 tggcgtaatc ctcagttttg aaggggagag gctgcgcatc cctttcgatg aattgacggg
    33061 agggcgccac atcactattt atggtcagca ggaggtggtc aaagacctgg tcgctgcctg
    33121 cctggaaaga cagattgaaa tccgctttga agccaaagcc tgggaactcc accggataga
    33181 aaccgaggct ccctatatcc agtatattca agacgggacc cggaaatcgc tggcctgtga
    33241 ttttatagcc gcatgcgacg gattccacgg catcggaaga aagtttttac cgcccggctc
    33301 ttatattgac tatcagacgt cctatccttt cagctggctc ggcatcctgg cggaaaccgc
    33361 accatccagc gaggaattga tctatacctt tcatgaagaa ggctttgcct tgcacagcct
    33421 gcgcagcgat gccatcagcc gtttttacct ccaggtggac aatacggaaa aactggagga
    33481 ctgggacgac tcccggatct gggaatctct cgaaaaaagg ttgttcaccc cgggcttcaa
    33541 gctccagacc ggccggatcc ttgaaaaatc catcaccccc gtgcgcagtt atatgaccgg
    33601 gaccatgcaa tccggaaggc tgttcctggc gggagatgcc gctcatattg tacctcccac
    33661 aggcggaaag gggctcaacc ttgccgtggc cgatatccgc tggcttgcga aagctatgat
    33721 cgctttctat aaatcgggag atgaacaaaa gctccgctcc tacagccagg actgccttcg
    33781 ccggatctgg agggtccagg atttcagtca ttttatgacc caattgttcc accggattgg
    33841 agaacgtgaa agcctcgaat accggctgca gcggtccaaa tttgattatc ttaagatttc
    33901 cagggcctat cagacttccc tggcggaaaa ctacgttggt ttaaaagaaa tctgaccatg
    33961 cctttttgcg tctgccgggg acacctcatc cattataaat acctggacca ggggaaaaat
    34021 caaaccctgg tctttattca ttccctggga accgatttca ggatttggga tatagtagcg
    34081 gggaatttat tccccgtagc caatatcctc ctattcgacc tccgtggtca tggactctcc
    34141 ggcctcactc cctccccccg tggactggaa gacttcacgg aagacttgct ggggctgtta
    34201 gaatatcttg ggatccgtca acaaatcatc ccggtcggct tatccgtggg agggatcatc
    34261 gcgcaactgc tcatccatcg catcccggat cagatcccga aggtggtact ctgcgacacc
    34321 cgatacaaaa tcggcaacgc ccaaaattgg aacgaccgga tcgcccaggt caggcgcgca
    34381 gggatcgaaa gcatatcgga aggagtggtc agccgctggt tttcaaagga tttccatgcc
    34441 cgctttccgg aaacggtttt gggctataaa tacatgctgg agcgcacgcc ggtggatgga
    34501 tatatccaga cctgtgaagg ccttcgtgat gcggacacgg gggacattgc ccggaagatc
    34561 gcggtccctg cactatgcct tgtaggcgcc gaagaccttt cgactacccc ggaagaagtg
    34621 aaaaatctgg ccgaccttat cccgggatcg gtataccttg tactggaaaa ttcaggccac
    34681 ctgccctgca tcgataatgc agcgaaactg agcaggctga tcacagattt tattcaagaa
    34741 tcatgaaaga tcatctatac gaacaaggca tgcacacccg gcgctcggta ctcggggatg
    34801 actatgtgga ccatgccgaa tcggtcaaaa cggaattcga ctcggatttc caggatttta
    34861 tcacccggaa tgtctggggc tccgtctgga gcaggccggg acttaccaaa agggaacgga
    34921 gcctggtgac cattgccctg ctgaccgcat tgggccactt tgaagaactc acttttcacc
    34981 ttcgggcgag ccggaataca ggggcctccc ttgaggaggt gaaggaagtc ctccttcata
    35041 ctgcggtgta cgccggggtg ccggcagcca accatgcctt taagattgcc aaaaaggtat
    35101 ttgaaaacga gaccggatcc tcttcttaaa tgattgattt taatcaagcc tgaccatgag
    35161 ccattcttca ggacgcgact ggacggtaca tccgcctttt ttatacccgg actataaatc
    35221 caccgtttac cgcgcccctt ccaaaccctt gatcccgtta aaacaccgcc tgggtgaatt
    35281 gtccggtccg gtttacggcg aagaagtggt gcgccttcac gacaacgacc tcaccaaaaa
    35341 tgccgccgtc aacggagaac cccttggaca acgaatcatt gtggccggga gggtcaccga
    35401 tgaactgggc cggccgatac ctcttaccct cattgagatc tggcaggcca atgccgcggg
    35461 ccgctacctg cataaaacgg accagcaccc ggcaccgctc gatcctaatt tttttggagg
    35521 cggacgatgc cttaccgacg aggagggtta ttataaattt tataccatca aacccggcgc
    35581 ttatccctgg ggcaatcacg ccaacgcctg gaggcccaat catatccatt tttccctgtt
    35641 cggccggaac atcagcaacc gcctggtcac ccagatgtat tttccgggtg atccgctgct
    35701 ggattttgat cccgtcttta aaagcgtacc ggaagcagcc cggcatctgt tgatctcccg
    35761 tttcaacatg gattttacgg aagaagcttt tgccctggga tatgaattta atatcgtact
    35821 cagggccact cccttcgaat aagtaatagg atttatagga tttataggat ttataggatt
    35881 attaggatta ttaggattat taggattatt aggattgaaa atcaccatgc ctcgcttacc
    35941 gcaaacccct tcccaaaccg tgggccctta ttttgcctat ggcctcacct cccaacaata
    36001 cggctatccc ttccccagcc tcaacgacgg catgatgatc gacgatagca tagaagggga
    36061 aaggatcacg atccggggtc gggtctttga tgggctggag gaaagcatcc cggatgccat
    36121 gatcgaactc tggcaagccg atgcccgggg ccaatacctc cagaccggac tgggcaggca
    36181 actgctgttt ggcaggcagg gtacgggcag cctcgaaagc cgggagtttg tatttcagac
    36241 catcaaaccc ggaagttccg ccggacaggc tccggtcatc cacgtctccg tattcatgcg
    36301 cggacaactg agccattcat acacccgcat ttattttgca gaggaagtcg cgctcaattc
    36361 gagggacgaa gtcctccttt ccgtaccgcc cgaaagacgg tccactttat tggtgggccg
    36421 gtctgtcata cacgggatgc cggtttatca ttttgacata catatgcagg gggagaaaga
    36481 gacggtcttt ttcgatgtct gatggggtta tttcgcacgg agtacacagg tttagttcgc
    36541 acggagcaca ctgagttgta aggaaggttt tgaggattgt tcggacgaag cttaccattg
    36601 tttggacgaa gctttggccc attgttggga cgaagcttgg cttcggcctg gtactaatgt
    36661 tgggacgaag cttggctcat tgttcggacg aagcttggct tcggcctggt aaaccaaaac
    36721 cctcaacact taactctgca ccctttcggc atttttaata atcacccgca gccgttcgcc
    36781 ccccttaccc taaatttgat ccccgacatg aaaaccgtcc ccatcctctc ccttgaatcc
    36841 gccgtctccc tcgtcaaatc cggggaccgg ctcctggtgg gtggcttcgg catgaccggc
    36901 aatcccgtgc atttgttgca cgcccttgcc gaaacccggg tgaaagacct gacctacatc
    36961 gccaacaaca ctggcgaacc gggcctgggc ggcggccgcc tcttgcgaaa tggccagatc
    37021 aaaaaaatca tcggctcctt ttttaccagc aatcccgatg ccgtggccgc agcgcaggaa
    37081 ggccgggttg aatatgaact ccttccccaa ggcagcctcg ccgaagccat cagggcggga
    37141 ggggcgggga tcggcggctt ctacacccct gcttcggccg ggaccttgtt ggccagggac
    37201 aaagagacca aatggattga cggcgtcgaa caggtcttca tcaaaggcat tcgcgctgag
    37261 gtggccttca tccgggcctg gcgggcggat acctccggca atcttcaata ccggatgacg
    37321 gaacaaaatt tcaacaaagc catggccacc gccgccgacc tcgtgatcgc ggaagtggaa
    37381 gaaattgtcc cggtgggcca tatcgcaccg gaggatgtcc acactcccgg gtgttttgtg
    37441 gactatctca ctcatgcaca cctgactcag gaggatctgg gtatatcggc cagcctgcaa
    37501 agttcaaaaa aggaaaatga cctgcgtaca ctcatcgcag aacgggcttt ccgggaactt
    37561 aaaagggggg acgtggtcaa tcttggcatt ggcataccca ccctggtggc tgattttatt
    37621 tcaccggacg acggcattat tcttcactcc gaaaacggca tgctgggcat cgggcccagc
    37681 cccaaggatg gcggaggggc gatgcactac cccgtcaatg ccggtaagat cccggtgacc
    37741 gcccttccgg gcagttccta ttttgacagc gccgattctt ttggaatgat ccgcggaggt
    37801 catattgacg ttgcagtgat gggaggtctt caggtggatg aaaaagcaaa cctggccaac
    37861 tgggcggttc ccggaaaacc cctcctgggc gtaggtggcg ccatggacct ggctgccggg
    37921 gctaaaaaac tcatggtcct catgacccat acccaccccg acggctcgcc taaaatcgtc
    37981 cgtgaatgca ccctgcccct gactgcaaaa aacgtggtca ccatgatcat taccgatctg
    38041 gccgtcttcg tattccgcga gggaaaactt accctggccg aattgatgcc ggggcacggc
    38101 ctggaagagg tgagggaaaa gacggaagcg gaatttgtgg aggattaaaa attcttatta
    38161 ttcattcagc cattaaattt gaacattcaa aaagaattga aagaaggatg caaattaaat
    38221 ttttatatag ctaaaagaag cgcatcatcc attctaattt aggagcattt aattggttct
    38281 tacaaattcc atttttgtca gatcactttt caattttaag agtcggtcta gcttggaggt
    38341 aataggcaat aataaattct caagtttaat agtaatctcg gacaggtttt cgccttcggc
    38401 ccttgacctg cgattgaaaa gttttctgat cacgcctgcc ataatataac tcgctttaat
    38461 gctgatattc tcagcattga tcaattgata accccgtggt ttcaatatat ccgcaaaagc
    38521 gtttaaaggg cggccaatgc aatagtcggt tacataagtg gctttttcta ctctttcaaa
    38581 taaatatatt tttcccgaac acaccctggc catttcttct aataatttag aaaactggcc
    38641 ttcatctaaa ttatgttgca aaactgtagc cgaaaacaca acctcaaaag ttccgtcccc
    38701 gaatggcaat aatcccaacg atgatttcac aagctttacg ggtgggggta aatttctttt
    38761 tgccacttcc aacatctttt ctgaaatatc cacaccgcag agctctctag gttgatgttt
    38821 taaaatttcg atcagatttc caccgggtcc acaacccact tcaaggaccg atttatcctt
    38881 aaattcgatt ttattcagca tttgcaggaa aagatttctc ttgtaccgat aatagggttc
    38941 atcatctcct gccaaaaaag gaccaccatc tctcaattca attctattcg ccactgcgga
    39001 ccaataggtt tcgggattgt aatttttcat taataaacgt tccttttgtt caggcaaaat
    39061 aaaatagttt tattgagacc ggagatataa ataaaaatat taatataaat aaaggattga
    39121 atttcaaact ataatgtttt actatcaaca ttacttccca gtttaggtgc ctccttcctt
    39181 accttacctt tatattatga aaggccttct tagtaagcga caaaatagaa tagacattgg
    39241 tttatggaca gcgtttctct ttcttttttc aacctccctt ctcagccaag tccggatagc
    39301 cggcaacctg caaaatgaaa ccatccgtta cggtttaaag cgattatcgg aacaaacggc
    39361 catcagcctg gatgaaaccc tcgatcttca aaaaagtccg ggccggagac ccgaccttct
    39421 ggtcctggct cccgatgacc tcaaaaatta tttgtacgtt ggcatccagg cttatgaatt
    39481 tgatgcggtc aaaacagaag ggttccggtt taaatggctt tccgaccata aaaccctctg
    39541 cctgttgcac agggacccgg taggagcttt gtatggaatc ctggaactca aggaaagcct
    39601 ggaatcgggg aaagcctggt catcccttcc ggaaaaaact tccaatcccc acctggaata
    39661 ccggatcatc aaattcaacc tgccctggtc gccctaccgg aataatgagg ccgttaccct
    39721 ccatacttcc acctgccggg acctgaaatt ttgggaaaaa ttcctggaca ggatggtgga
    39781 caaccggtgg aatgcacttt cactctggaa tatccacccc ttccctttta tgatcaggtc
    39841 aaaatccttt ccctttgcca atgaatttac cgaacaggaa atgcaggatt ggaaacaatt
    39901 ctggacttcc ctttttgaaa tggcaggcac ccggggaatc cagacttttg tgatcaactg
    39961 gaacatagcc gtctctcctt cctttgccaa aaattatgga gtgcctgaat acagcagcct
    40021 ttcggatacc gtcatccggt atacccgcga atcggtcacg caactcatcg atgagtaccc
    40081 cggcctcacc ggcatcggcg tcaccctggc cgactggatg ggcacttttg atgaaaaaat
    40141 gacaccccaa cagcgggaag actggattga aaaaactttt gtcgcgggca tgcaggccgc
    40201 ctcccgtccg gtaaaattta tccaccgttc cgtactggcc ggtgatccga tggccatgcg
    40261 cgcacttctg gaaaaggcaa aactgaaaga tccggccctt gtcgaaataa agttcaactg
    40321 gtcccatggc cactccaccc caaggctcgc catcacccat gattatcatt cgggcaaact
    40381 ggatgagcgg ttctggactc ccaaaccgga ggattaccat atccagtgga tgatccgcaa
    40441 tgaggacttt tttattcttc gctggggtca ggcggatttt atccggcagc atatccatca
    40501 aaacagcaag gattatgtca atggatattt tatagggtcg gaaggatatg ttcccgccct
    40561 ggactattat accatccggg atggacagga cgcgccccaa tatgccttcg aaaaccaatg
    40621 gttgttttat accctctggg gccgcttatt gtatgacccg gccttgcccg atgaattttt
    40681 tatcgccctc ctggacaaac gatatggaaa gggatccggg aaaaccttat ggcaagccat
    40741 caacctggga agtaaaatac ccctgcgcct ggcttctttt ttccggtcca cctgggatta
    40801 taccctttat tccgaaggct tccttgcccc ggaaccttcc aaccctgatg gtttttttga
    40861 ccggacctcc cccttcattt ccattgacga actcatccat cacgaaaccc tggatccgaa
    40921 aatgatttcc atcgaggact atagcaagct gaaaaaagaa aataaaaaag cgccggacag
    40981 tctgatcagc cctctccaaa tcgcccggca atcggaaaaa gacagtaaaa aagcactacg
    41041 agggtataag gcctggacaa aaaggaatcc atcggccgca cctccccttg cttccgaatg
    41101 ggtcgacgcg gcaacctggg cccgcctcgg gctctatttt gcacacaaac tccgcgccgg
    41161 tgtggctttg cattcgtttc gtcaaaccgg ccggatttca gaaaaacaca aagccatccg
    41221 tgaactggaa tccgccctcg ttcaatggaa ggaagtggcc ctcctcacca aggatcgtat
    41281 taaagccacc ccccatgtgg cccttggccg ttatcgggat acctttccgc ttttttcctg
    41341 gcaggctttg ttgccccagg tggagaggga tctgcaaata gcccgcgatg cccaaaaagg
    41401 attgtttcct caaactaaaa ctcaatgacc attgtggttt cagcaatgag gattccctca
    41461 atcctcaatc ctcattcctc attcctcatt cctccatcct ctaacctcat acctcaaacc
    41521 tcaaacctct ctatgcccaa acccagctcc ttctccacct accacaagat catcatcttc
    41581 cttcttgccc ttacccagtt taccattgtc cttgatttta tggtgatgtc ccccctgggt
    41641 gatttcctga tcaaagccat gcacatcagt ccgggacagt tcggcatcgc ggtatccgct
    41701 tatgctttca gcgccggaat atccggcttg cttgcggcgg gttttgcgga ccggtatgac
    41761 cgtaaaaaat tgctcctgtt tttttattcg ggttttattt tgggtaccct tgcctgcggc
    41821 ttggccacca catatccctt tttgattttt gcccgcattc tcacaggctt gtttggcggg
    41881 gtcatgggtt cggtgctgat ggccatcatc ggtgatgtat tcaagccgga gctccgcgga
    41941 cgcgtcatgg gttttatcca gatgggcttt gccgccagct cggtactcgg cataccggtc
    42001 ggcatctacc ttgccaatat ctgggactgg catgccccct tcctcatgat cgtagggctg
    42061 gccctcctga tcttcattct ggtctaccgt tttatggagc ccgtcgtcca ccatttgtcc
    42121 cagaaccaga aaaccaccgc actaaaccat ttcaggaatg ttctcgccca cccgccgtat
    42181 caactggcat ttttggccac ggctttgctt tccatcgggg gtttcctaat gatgcctttt
    42241 ggcagcgcct tcgcgatcaa caacctcaaa ataagtccgg accaactccc tatcctttat
    42301 atggccacgg gcatcgcttc cctggtgatc atgcccctgg tcggccgctt gagtgaccgc
    42361 accaaccggt ttaagatctt cttattcggt tcggtctggg catccatatt catcgtcatt
    42421 tataccaatc tgcctgtagt tcccttatgg gccgtgatcg tcctgaatat tctcctcttt
    42481 gccggcatca tgtcccggat gatcccggca tcgaccatca tgatttccct gccccgcctg
    42541 cacgaccgcg gggcgtttat gagcatcagt tcctccctgc agcaaatcgc cggcggcatt
    42601 gctgccgctt ttgccggcct gatcgtaacg cagaaaacgc ccaacagccc gattgaacat
    42661 tatcctaccc tcggatttat cagcgtggcc gtcatgatgc tctgcgctta cctgatatac
    42721 aaggtctatc tgatgacccg gcaggcatca aaagaaggag tgtcgcaagc caaaattgaa
    42781 caataaaatt tttagaattc cctttcccct ttcccctttc ccctttctcc cttcctcctt
    42841 aaatcacttt ctccttatct tgcaatcagt agtcagattc catgatatct gataaaagaa
    42901 ggtcttagct tcgggaagcg
//
LOCUS       DAOQXT010000032        40964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1086_length_40964_cov_6.890049, whole genome shotgun sequence.
ACCESSION   DAOQXT010000032 DAOQXT010000000
VERSION     DAOQXT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 40964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40964
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1086_length_40964_cov_6.890049"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PKM27_07565"
     CDS             <1..1291
                     /locus_tag="PKM27_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNR07157.1"
                     /translation="LTPSQDITPLMEGILKYVPAPVVEEGNLQMQLTSIDYSSYHGRI
                     GIGKVSRGSIKENQPVSLVQANGTVKTRIKDLFVFEGLGKKKTNEVFAGDICAVVGLD
                     SFNIGDTIADYENPEGLPTITVDEPTMHMTFSINNSPFYGRDGKYVTSRHLKERLAKE
                     TEKNLALRVEESGSADTMLVYGRGILHLGILIETMRREGYELSVGQPKVIVKMIDGKR
                     YEPFELLVVDVLQEYSGKVIDLVTSKKGEMHVMETRNDIQHLEFEIPSRGLIGLRSRI
                     LVQTAGEAIMSHRFTEYKPWKGPMSGRINGVLIAKSIGQATAYSIDKLQDRGSFFIEP
                     GDEVYTGQIIGEHTKPGDLVVNACEPKKLSNVRASGSDEAARIFPKIDMSLEECMEYI
                     EADECIEVTPKSIRLRKVFLGEEERKKASKSAQIEMA"
     gene            complement(1412..2212)
                     /locus_tag="PKM27_07570"
     CDS             complement(1412..2212)
                     /locus_tag="PKM27_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNR07158.1"
                     /translation="MYYLASKGLSFLLHPFHWVLALLLLSVLVRPARLKKSMRIFSLI
                     LFMIFGNRAMINTVYRRIEAKPLLQESISPPFEYAVVLGGGFAGLHNGFPDRIQFNDH
                     VNRLTEGMELMGSGKVRKLILSGGEGGLQRDKAAESLLSQAFLTEINWPDSTILTEST
                     SRNTFENARNVKRMLDSLSALAPAIEGPGTRAPVLLITSALHMPRAAACFHRQGIPFV
                     PYPADYQQWEKVSLPLYFFPQLRCFDEWQAIFREWVGTAVYRLKGYIE"
     gene            complement(2220..2810)
                     /locus_tag="PKM27_07575"
     CDS             complement(2220..2810)
                     /locus_tag="PKM27_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNR07159.1"
                     /translation="MNTISSVCVYCASSNQVDSRFFEAAERLGEALASQNITLIYGGG
                     STGLMGRIADRVLARGGKVIGIIPDFMEKVEWAHRGITELHIVGDMHERKKSFMDRAD
                     ALVALPGGSGTLEELMEAISLKRLGLYNKPIVILNTVGFYDPLLAQLQRCIEERFMNP
                     DSAEMWSVVAHPEEVIPAILRAPSWHTNAIERAALK"
     gene            complement(2902..3945)
                     /locus_tag="PKM27_07580"
     CDS             complement(2902..3945)
                     /locus_tag="PKM27_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNR07160.1"
                     /translation="MAKPLHNRQNRKELKEKYASIRESRIVLSFYRYVFIRQPETFRN
                     ELYQNLDRLDVKGRIYIAQEGINGQISLPEKNLEALQSLFQNLEALRDIRLNLAVEHQ
                     ARSFFALIIKVKPKIVADGIEDPAFDVTRSGVHLDAEAFNRLALRPESVIVDMRNHYE
                     SEVGHFRNALLPQVVTFRESLPIVKDLLEDKKDQPVIMYCTGGIRCEKASAYLKHHGF
                     KQVYQLDGGIIKYSHDAAAKGLPNLFLGKNFVFDERLGERISEEVIARCHQCGSPCDD
                     HTNCANDACHLLFIQCKSCAEKHRGCCSEKCSDFIQLSPEEQKQGRRTEVFNGTKFSK
                     GRYHGGQRLDGRG"
     gene            complement(4120..5886)
                     /locus_tag="PKM27_07585"
     CDS             complement(4120..5886)
                     /locus_tag="PKM27_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR07161.1"
                     /translation="MKKYFLLLVFIGMYFSCSEDFFDLNPQGRASLNQLSNKSGVNAL
                     LIGAYSLLDGVGAGNTGRQSTVSNYVFGGISSDDAVKGTDAGDQPEQSFIEQYNWIAD
                     NTYFLGKWWHSYDGVARCNDVIQLANSEAVKDMTPAEKTQAIAEARFLRGHYHFEAKK
                     MWNKVPYIDETIYVATDANSTKIPNSEDIWPKIEADFDFAAKNLPPVQAQKGRATQWA
                     AKAYLAKALLFQKKWAAAKTLLDDIVLNSGKKLMTNYHDNYRTATNNNAESIFEVQFS
                     VNDGTNGGNGNAGDNLNWPYSATAPGRGCCGFYQPSHNLVNAFKTDEKGLPFIGAAAD
                     GSLDTYNKVDLPNDQGISAGTAFTLDKSVPVDPRLDWTVGRRGVNFLDWGPMPGSTWI
                     RDQAYAGPFTGKKWMYYLAEEGSTTHSTSRRNVNNNYRLIKLSSVLLWLAECEVELGN
                     LSAAENLVNHIRNRAKNGSKQDGTVTYKVEPYEAGTFSAQGADFARNAVRMEQRLEFA
                     MEGHRFFDLVRWGIAEKVLNKYAAEEAVPGTEPSGRKFNKRGYMAGKVFGAKNNYYPL
                     PQDEILNSQKDGQPTLTQNPGY"
     gene            complement(5913..9119)
                     /locus_tag="PKM27_07590"
     CDS             complement(5913..9119)
                     /locus_tag="PKM27_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR07162.1"
                     /translation="MFLFLGLTVQAQRTINGKVIAGDDHSGLIGATVMVKGTTTGTST
                     DIDGNFTLEVPDGTTSLVISSIGFLTREVDVTNTNSVEITLPSDVSTLEEIVVTGYAS
                     QRKKDITGAVTVVNSKDLTAVPTASVTQMLQGRASGVNVGNDNSPGGGTMVRIRGFGS
                     INNNSPLYVIDGVPTQGTLNQLNPNDIESMQILKDASAASIYGARATNGVVIITTKKG
                     KVGDPLITFDFYTGTQRPGKTLDLLNTQELGQYLYLADIGAGKNPSVTSPSAQYKFGP
                     NGEQTIADYIYPNIYGELPANYTYTNNIADPNLGRTAFNITKANKQGTDWQDVIFDPA
                     PIANYQLGASGGTKFGRYSISANYYKQDGILRYTNYDRYSVRANTEFTKGKLTFGENF
                     TYSYDERQGITNNNESNPIMFAIRVHPIIPVFDITGGPPELGGTNTSPYNGFAGSRGV
                     NLGNAPNPLARLYREKDNITKGSHVFGNVFAELSILDNLKARTSLGIEYNLFNRSEFF
                     HRDIEAAEARNANSLNVLNNTDRSLTWYNTLNYDKTIGDHTFNVLLGTEAVTTNAVGF
                     VASRSNFAFDDLDYRYLDAGSSAGLSNSGAGATLSALFSQFGKVNYAYKNLFLADFTL
                     RRDGSSRFSANNRWAIFPAFSAGLRLTELGFMKQIGAVNDLKIRAGWGKTGNQLIPNV
                     YNAYTLYAPDPINNAYDINGTGSTIVGGFDLVQFGNSNGKWETNTSTNIGFDAALLDS
                     RLEVVFDWYNRITSDMLTQIAPPRTAGSGTVPYTNIGEIRNRGLDLGINFRENVGNFR
                     YLIGVNFAHYKNEVLKLNNDPNATVFGFATRLPAISATRAGLPIASYYGYIVDGVIKD
                     QAEADAAPKFGTYTKEGTFKFRDVNGDNVITAADRTIIGDPHPDFTYGINVNVGYKDL
                     DLTLFAQGSQGNEIFNYVRYWTDFNTFQGNRSKELLNDSWKKSGDIAKLPRLNSGDAT
                     SQQISTYFIEDGSFLRLRNVQLTYSVPNSLTRKAGISNAQVYIQGQNLVTLTKYTGLD
                     PDINLRTSGTDNQDIHMGIDEGAYPVAKSFIFGVRLGF"
     gene            complement(9232..9669)
                     /locus_tag="PKM27_07595"
     CDS             complement(9232..9669)
                     /locus_tag="PKM27_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HNR07163.1"
                     /translation="MYPILKHLHSGVRWLVLFALLWAIYDAWQKWKQQATLNKDTRGP
                     SFLALNLCHLQLLIGCILIFFTPRIEAGPSMMKDKVMRFFAVEHPLMMLLAVALITIG
                     FVKAKKNSSGQSFRILFWYYLIALAMILFAIPWPFQAYTTGWF"
     gene            complement(9731..11308)
                     /locus_tag="PKM27_07600"
     CDS             complement(9731..11308)
                     /locus_tag="PKM27_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNR07164.1"
                     /translation="MNGKAGLAGEGIYDYIVVGSGFGGSVSAMRLAQKGYSVLVLEKG
                     RRYRDEDFPKSSWNFKKYLWMPFFRWTGFLKLTVFKKLFVVSGVGVGGGSLVYANTLL
                     EPPDPFYENIPLKTRDWKKALEPFFKTARRMLGVTPARNQNPDDLLLREIARDLGRES
                     TFQGVDVGVYYDSDPTPRDPYFNGEGPLRNPCIECAGCMVGCRHNAKNTLEKNYLWFA
                     EKYGATILPLTQAERIEWNQEQSVYTIRVRSPFKKKINGEFKARGIILSGGVLGTLEL
                     LFRQKFRYGTLPGLSDQLGRHILSNSESICGVIGADRKLNHGVAISSIIQADPDTQIE
                     LCKFPDGSGSLFRLAFLTAHGGGPPIVRSIKILAKIFLRPLDTLKWIIRGQDSRRGVV
                     ILVMQHIKNALAMRWTGNGLRLVSDGDEKPIPVFIPAGYDFMQRLAKKTKAVPVSAVT
                     ETFFNMSTTAHFLGGCPMGASVNEGVVDDAFRVHGYPGMYILDGSIIPVNIGVNPSLT
                     ITALAEYAMDQIPIKSD"
     gene            complement(11280..12404)
                     /locus_tag="PKM27_07605"
     CDS             complement(11280..12404)
                     /locus_tag="PKM27_07605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR07165.1"
                     /translation="MFVPALLVFLAYCALIITGIYSWKKAEEQKAQRAIVPRPPAFFS
                     VLIPFRNEAQNLPGLVENLRNQSLPSESWECILIDDHSTDQGLAFLADAGLPTNFRII
                     SNRGEGKKQAIAEGLKAAKGEYLVQADADCFYGRHWLETICTRIAGSGLLMVTGPVYI
                     PGSETHTLLDYFQAFEGIALMGMTQAGIYSGGWHLANGANLAAHRSVYDYMRASDPEN
                     PFASGDDLFLMQAAVKMDKSRVGFLFEPSALVGTRPLKNIHSFIQQRLRWGSKNHALE
                     DWKIKLALFIPILCAMTMVGMLAYSLWSGQAWVFTVSFWAGKFLIDYLYFKALSPFFR
                     LRISPFSFLTCALVYPFYLLIIVLLSIFKRKYEWKGRLGG"
     gene            complement(12500..12913)
                     /locus_tag="PKM27_07610"
     CDS             complement(12500..12913)
                     /locus_tag="PKM27_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07166.1"
                     /translation="MENRRYTTTETIVGLVFFALVLWAAFFLFKSIFRVLAWAAPILF
                     IAALIINYKLVVSYGKMLVVLLQRNTLVGVIAIILTFVAFPIVAALLFAMALMNKKAD
                     NILKEEIRNKEGIPTDYTEISSEAGKQYDDLFGKK"
     gene            complement(13030..14790)
                     /locus_tag="PKM27_07615"
     CDS             complement(13030..14790)
                     /locus_tag="PKM27_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR07167.1"
                     /translation="MADSKKKFNYSLLWRVIQLSIPYKKLFYWTLVLAVIQAPLGILR
                     PHLINVMVDNHVMKDELGGLGPLVFWYMVVLLATVWLRYIFILWTNTLGQSIIRDLRT
                     RVFNHIMDLRMAYFDKTPIGTSTTRTINDIESINEVFSEGVITILADILGLIAVLIIM
                     FVVSWKLSLICLTTLPFLLYASYLFKEKVRVSFQQVRTHIARMNAFLQERISGMRIIQ
                     IFNAEKQEQEAFRKINREYTQANIDGIFYYAVFFPVVELISAASLALMVWWGSRGVLE
                     GEVTLGALVAFPIYLTMLFRPIRFMADKFNTLQMGLVAAERVFGVLDNQSKIKNEGIL
                     VPQRLQGDLVFDKVRFSYDGEQEVLKGISFHVPAHSTLAIVGSTGSGKTTLISVLNRL
                     YDIQGGEIRIDGKNINEYELVALRRRIGVVLQDVFLFGGTVLENITFRQSGIPSETVI
                     RAARQIGAHAFIEKLPGGYDFVVTERGSNLSMGQRQLISFIRALVFDPDILVLDEATS
                     SIDPETESVIQYAIEKLIEKRTSIIIAHRLSTIRHADQVIVMDQGQIKEIGTHEELLD
                     KPDGWYRKLHDYQFAKMAEL"
     gene            complement(15007..15696)
                     /gene="radC"
                     /locus_tag="PKM27_07620"
     CDS             complement(15007..15696)
                     /gene="radC"
                     /locus_tag="PKM27_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNR07168.1"
                     /translation="MDENKKSIKYWADQDKPREKLVQKGPFALSDSELIAILIGSGTR
                     EHSALDISRDLLRRIGHNLNELAGLSHQELKKIKGIGPAKAIAIHAALELGRRRQGAA
                     PEYRKKIQSSSEAASLFSHLKDSRQEEFWMACLNRQLQVLYLGQIHLGGISSVSADPR
                     IIFGKALEHQAVSLIVSHNHPSGNLRPSKEDEAITKKLKAAGELLDIRLADHLIISRE
                     GFFSFADEGMI"
     gene            complement(15689..16834)
                     /locus_tag="PKM27_07625"
     CDS             complement(15689..16834)
                     /locus_tag="PKM27_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNR07169.1"
                     /translation="MKFATKLIHAGIEPDPTTGAIMTPIYQTSTYVQEAPGKHKGYEY
                     ARTQNPTRSVLEANLAALENGDYGICFASGLAAQDAVMKLLNPGDLILSTNDLYGGSF
                     RQMRRVYEKFGIQSRFVNLSDPEVAREHIRPGVRLLWVETPTNPMLNIIDLELVCGLA
                     KQHGILVCVDNTFASPYLQNPLDYGADIVLHSATKYISGHSDVVHGALIVKDKALADS
                     LHFIQNATGAVPGPMDCFLVLRGIKTMHVRVQRACQNAARIAHHLDAHPKVDKVFYPG
                     FASHPNHGTAKKQMRDFGAMISFDLKDNRIEHANTVLSNTHLFSLAESLGGVESLIGH
                     PATMTHASIPREERLRTGLTDSLIRLSVGIEDVDDLIADLDLALSHG"
     gene            complement(16967..18298)
                     /locus_tag="PKM27_07630"
     CDS             complement(16967..18298)
                     /locus_tag="PKM27_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR07170.1"
                     /translation="MSKRRKFLKQASMAALGTSLLAQGNTAPFLIYKRKASPSDSLRF
                     GTIGINGMGWANTRAALTQPNAQLVALCDIDQSVLDKRMAELKDLKVDAAKIKTFKDY
                     RKMLEMKDLDVIIIGTPDHWHPLITIHGCQAGKDVYVEKPIANSIGESLAMEQAQKKY
                     NRAIQVGQWQRSQKHFRDAIEFVHSGRLGNIRTAKAWAYQGWMKPAPVVPDAPVPAGV
                     DYDMWQGPALHRPFNTSHFHFNFRWFWAYAGGLMCDWGVHLIDYALFGMKAIMPKTVA
                     SLGGKFAYPELSHETPDSQHTIYEFDHFILSWEHAMSIDNGPYGRDHGISFIGNNGTL
                     VLDRGGWEVIEEKREKNKILIPLQKREDNGVEKHMENFFGVVRSRKMEDLNAPIQAGS
                     LVAKVCQMGNIAFRSGKKLDWNANQQRFTDEAVNKEYLMPEYHNGYSLPVV"
     gene            complement(18374..18446)
                     /locus_tag="PKM27_07635"
     tRNA            complement(18374..18446)
                     /locus_tag="PKM27_07635"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18411..18413),aa:Gly,seq:ccc)
     gene            18920..19528
                     /locus_tag="PKM27_07640"
     CDS             18920..19528
                     /locus_tag="PKM27_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNR07171.1"
                     /translation="MDTAVKSPVMLYTEQTPNPETLKFVLNRIIYKGIADFKQADLAL
                     DWSPFASALFEFPFVKGVYVCNNFVTITKENTVDWNDIMLELKEYIKAYVAENKPVIR
                     EGYQEFMEQQERENSTIEYSEADAELVKRIKDLIETYVKPAVEMDGGNIEFKAYDQGT
                     VYVIMQGACSGCPSSTVTLKAGIEGMLKRMIPEVKEVVSEMG"
     gene            19532..20317
                     /locus_tag="PKM27_07645"
     CDS             19532..20317
                     /locus_tag="PKM27_07645"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNR07172.1"
                     /translation="MDAALLPEVDKLVRETGEWILSQQAVFTDSDIEEKSLNNLVSYV
                     DKTAEKKLVEGLRVLWPQAAFLTEEGTVHYQPAEWEWIIDPLDGTTNFLHQIPVYCIS
                     VALRRNQEIVLGVIYDPNRRESFTALSGRGAHLNGKPIRVSTTPSLQSALIATGFPHR
                     NFEGMDAYIRSLEYFFYRTRGLRRMGAAAIDLAYIACGRMDGFFELKLNPWDIAAGVL
                     LIREAGGMISDFGGSDDFLFKSELLAGNPAVYLEMKEVLAKYF"
     gene            20424..21659
                     /locus_tag="PKM27_07650"
     CDS             20424..21659
                     /locus_tag="PKM27_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07173.1"
                     /translation="MLYENVRMALSSIKANLTRAILTLMIIAVGIICLVGILTAIDSL
                     IFSLSDNFSSLGANSFSISPSGEAMQARREGRMEKRGEEITFQQAMDFKEKLNIDGRV
                     TVHFNGTSRAALKFENEKTNPTITVIGVDENYLTVNGYDIEFGRGFTNLEIQNGAPRV
                     ILGTELVQTLFKGRGPAALNQTIQVGNIRVQVVGVLKSKGSSLNSSSDRIVLMPLLQA
                     KQYYASPNENFNIAVQVNKVSGMDDAINEATGTMRVVRGLRVNETNDFEIQKSEGLVN
                     IIKENTVKIRLATIGIGLITLLGAAIGLMNIMLVSVTERTREIGISKAIGATSRNIMV
                     QFLTEAILISLMGGLVGIILGIFVGNIVTLLIGGKFLVPWNWISLGVFVCIVVGVFSG
                     LYPALKAARLDPIESLRYE"
     gene            complement(21685..22956)
                     /locus_tag="PKM27_07655"
     CDS             complement(21685..22956)
                     /locus_tag="PKM27_07655"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNR07174.1"
                     /translation="MGVDVLLGLQWGDEGKGKIIDKVCKDYQVVCRFQGGPNAGHTLY
                     AADQKIVLHTIPSAILHEGIDNLIGNGVVIDPVTFEKETDKLKGLGYEIAHKLIIARK
                     AHLILPSHQLLDIASEQSKGLNKIGSTLKGIGPTYMDKTGRNGLRVGDLELAGFDKKY
                     QVLKEKHLDLARIYPEVEYDLEKAEEKWFASLENLRAFRKVDVEYYIDQALREGRNVL
                     AEGAQGSMLDIDFGTYPYVTSSNTISAGVCTGVGVPPGKIGKVIGITKAYCTRVGGGP
                     FPTELFDETGDQLRKAGHEFGSTTGRPRRCGWIDLVQLNYSIMINGVNELIMTKIDVL
                     NDFDTIRACDSYLDGQGEKTKTIPFDLDHSGYQPQYLSFPGWGTSLHEAQTFDQLPGA
                     ARHYIESLENLLRRKFSLISVGVGRDEVIER"
     gene            complement(23145..23630)
                     /locus_tag="PKM27_07660"
     CDS             complement(23145..23630)
                     /locus_tag="PKM27_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNR07175.1"
                     /translation="MNFEQVKNIFSAHLDKGGFRKTPERYAILEEIYNRSDHFDAEAL
                     YIEMKNKNYRVSRATVYNTLELLVNCEMVKKHQFGKNLAQYEKSFGYKQHDHLICLDC
                     GKVLEFCDPRVQQIKTTMAELLHFKIEHHSLNLYGTCQVDCERKKSGQLLRTKMEPVV
                     V"
     gene            complement(23641..25890)
                     /locus_tag="PKM27_07665"
     CDS             complement(23641..25890)
                     /locus_tag="PKM27_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNR07176.1"
                     /translation="MSPETTLTASIHVEDEQDLHLIQSAYRRLLKTFKSELEETDKIN
                     IRKAYEMAVVAHRSQRRKAGEPYVLHPIEVARICVEEIGLGPTAVISALLHDVVEDTP
                     VTLEEINKEFGPKIGMIVDGLTKLDGLYNLETAQAENYKKVLSTLITDVRVVLIKMAD
                     RLHNLRTISAMPRHKQLKIAAETEYIYAPLAHRLGFYNIKSEFQDICLKIQEPEAYEE
                     IARKLQDSESARNEYIERFIKPLRVELDQLNVPYKILGRPKSISSILNKIRKKKIPFE
                     EIYDLFAVRIIVDVPAKMERSLCWQIYSIVTDVHTPIPERLKDWITTPKANGYESLHT
                     TVIGPGGRYVEVQIRSARMDEIAERGFAAHWKYKGISNQQKLYDGWLDNIREVLESNH
                     ADAIEFLNEFKSNLFSEEIYVYTPKGDVKILPKGATALDFAFSIHTDLGYHCTALKVN
                     NKLVPMGYHLEHGDQVQIVTNNKQKPSEGWLKLVVTGKAKAKIRQALKEERKERADLG
                     REALERKFKNLKLDLEENLDALVRHYQYKTRMDLYFSIASGEVDISELNQVPVENGKL
                     LFNQAEAEPVATQAHPEVGPAQAPAPAKTSTGKTKLYINGEPAEQYEYSFASCCNPLP
                     GDDIFGYLTSSSGLKIHRSNCPNSTHLLANYGYRVVKAEWSDYSDSEFIAKLMITGID
                     DGPGVIEKISKAISNDLNLNIRSFSIEGKEGYFEGKISLMVKNKNQLQKAVSSLSKLD
                     AISTVERIE"
     gene            25967..26048
                     /locus_tag="PKM27_07670"
     tRNA            25967..26048
                     /locus_tag="PKM27_07670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26001..26003,aa:Leu,seq:tag)
     gene            26077..27543
                     /gene="tig"
                     /locus_tag="PKM27_07675"
     CDS             26077..27543
                     /gene="tig"
                     /locus_tag="PKM27_07675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNR07177.1"
                     /translation="MSVSTQTTGQLQATLTVHIPVDEYQATLSKELQNYKNKAQLKGF
                     RKGKTPVDLIKKLYGNAILKEVVENSLQKQLYDHIRQNNIHLLGQPLMNAEQAEIYYN
                     IQEPSDYTFLFDIGYAPDFEIQGLDHEFGFYKIVPSQESVDKEFENLRTRMGELKTVD
                     LPVEENHLVDFTAAELDGTELKEGGVQTEFSLWISQVDGPAKISILGKQKGDEIDLDL
                     RELHTGKDEEFIRKRYLTSLEEGQQVPSRFRLTVKDIRANEPAVTDQAFFDKAFGPDK
                     VKDLDEAKSFIQGVLANQYVSRSEALLFRDIQDHLLKTNKQDFPDAFLKRWLKSQNPK
                     LTDEGIEAEYDDFAQNLLWTIIRDKLIKRFDITVSAEEIKARFRQQVLNYFGGMALGD
                     TSFLEPVIQRMMQREEEVEKQHDEIMSDKVFDSLQATVTLVEKEISEADFEVLMEQAR
                     QETAARRAKAAKALAGHDHDHDHDHDHDHDHDHSGHSH"
     gene            27595..28296
                     /locus_tag="PKM27_07680"
     CDS             27595..28296
                     /locus_tag="PKM27_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNR07178.1"
                     /translation="MKENQEFRKYATGHLGMSGMHLDRYMSQVTVPDISGFTPTVIEE
                     RSLNVVGMDVFSRLMMDRIIFMGVPVNDYVANVIQAQLLFLESMDPKRDIQIFINSPG
                     GSVIDGMGIYDTMQYVSPDIGTICTGLAASMGAVLLAAGTKGKRTCLQHSRVMIHQPS
                     GGMQGAFKDMEISYKLIKTMKGELYSILSHHTGKSVEVIEIDCDRDNWMTALEAKEYG
                     LVDEVLNRDNPRKKV"
     gene            28324..29568
                     /gene="clpX"
                     /locus_tag="PKM27_07685"
     CDS             28324..29568
                     /gene="clpX"
                     /locus_tag="PKM27_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNR07179.1"
                     /translation="MAKNSESQAQCAFCGKHKNEVAILVAGLDAHICEMCIEQAHHII
                     REEFDTKKKKSKSYLIPKDLTPAKIKAHLDEYIIGQDEAKKCLAVAVYNHYKRLQIGS
                     SGDDVEIEKSNVIFIGQTGTGKTLMARTIAKILNVPFSIVDATVFTEAGYVGEDVESM
                     LSRLLQVCDYDVSAAQKGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKMLEGAE
                     VSVPPQGGRKHPEQKLVTVNTQNILFICGGAFDGIDKIIAKRINTQVIGYGAKDKEWV
                     DKENLLSYITHQDIKAFGLIPELVGRLPVLAHLDPLNKETLVRILTEPKNALVKQYKK
                     IFQQEQVKLEFTPEALDFIASKALEFKLGARGLRSMLEAILMDDMFEMPSSKNNKGEL
                     TVDLFFVQSKLEKSKLSKLKAA"
     gene            29635..30372
                     /locus_tag="PKM27_07690"
     CDS             29635..30372
                     /locus_tag="PKM27_07690"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HNR07180.1"
                     /translation="MRYFFECSFDGTPFHGWQRQAGQVSVQEIMENTLKIILRVPGLE
                     ITGCGRTDSGVHAEQFFAHFDHGADLPPGLEYHWNAILPREIAVHRVWRVGDGSHARY
                     DAVSRTYQYHIHRSKSPFLVNRSFCYRQYDRLDLDLIQQAASLLLQYSEFLPFCRSHS
                     SASHYGVRLQQAEWKEEKGPALVLTLTANRFLRGMVRLITGMCLNVGTGAVSIEEVKH
                     SLDTQTLLPKSFSVPAHGLYLTAVSYM"
     gene            complement(30514..31818)
                     /gene="glyA"
                     /locus_tag="PKM27_07695"
     CDS             complement(30514..31818)
                     /gene="glyA"
                     /locus_tag="PKM27_07695"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNR07181.1"
                     /translation="MTDPVFSLIEKELRRQRNGIELIASENFASYNTIRAMGTCLNNK
                     YAEGYPGKRYYGGCEVVDEIETLAIERLKTLFGASYANVQPHSGAQANSAVFLACLQP
                     GDTILGFNLSHGGHLTHGSPVNYSGKTFKPVFYGVEAETGRIDMDKVEQTALQHRPKL
                     IICGASAYSRDWDYARFREIADSIGALLMADIAHPAGLIAKGLLQDPLPHCHIVTSTT
                     HKTLRGPRGGVIMMGKNFDNPWGRTTAKGNKIKMSSILDSGVFPGMQGGPLEHIIAAK
                     AIAFQEALDESFKDYAVQVKKNAQAMARRFNELNYQIISGGTDNHLMLINLSNKNISG
                     KEAEEALGKAEITVNKNMVPFDEKSPAVTSGIRLGTPAITTRGLKEEECIQIVDWIEE
                     ILTHPDKEDLILGIKKKVNEMMAGFPLYPEIEAGSWELEARS"
     gene            complement(32138..33286)
                     /locus_tag="PKM27_07700"
     CDS             complement(32138..33286)
                     /locus_tag="PKM27_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR07182.1"
                     /translation="MNKRKIINDPVYGFTTLPSDLIYDLVHHPWFQRLRRIKQLGLSD
                     FVYPGAQHSRFQHALGALHLMVDAINILRSKGVDISREEAEGAQIAILLHDIGHSPFS
                     HALEKRLVPLDHEQMSILLIRALNQEFDGKLSEALKIFTNRHPKKFLSQLVSGQLDLD
                     RMDYLNRDSFFTGVAEGIIGYQRIIHMLNIHDHQLVVEEKGIHSIEKFLQSRRIMYWQ
                     VYLHKTVLAAEKMLIHWYSLLDRDPGLLRANPALKKLRSGQFRPEELPGLFASIDDTD
                     IVFALKSGLDSKDPELRTLAGGLIHRRLFKCMLSDKPFSPAVKKNIRNLHPGGMIFQG
                     KESNSNYKLNIKEIMILTKKQEVIPLSAFEGFQQMSQQETKYFMVYQG"
     gene            33370..34926
                     /locus_tag="PKM27_07705"
     CDS             33370..34926
                     /locus_tag="PKM27_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNR07183.1"
                     /translation="MESVKILWADDELDLLKPQVYFLEKKGYRFVLVSNGYDALEEYQ
                     QNKDFDVIFLDESMPGLSGLETLAKLKAINPNIPVVMITKNEAENIMEEAIGSQIDDY
                     LIKPVNPNQVLLTLKKIIDNKRLVQEKTSSDYQQEFRNISLRINEGPNLKGWIDIYKN
                     IVGWELKMDQAENPEMMQILAIQKTEANSEFFKFVTRNYLQWIEKAGAEVTLSNTLIK
                     DKVFPLLAQGNPVVMILLDNLRYDQWKMIEPRISERYRVIEEEVFLSILPTSTQYSRN
                     AIFAGLMPAQIEQRFEDWWLNDNEKGGKNLHEAELLERQLSRLSRTPVKHSYTKITQV
                     SDARNLPDLTSQIMNQALTVIVYNFIDMLSHARTEMEVLKELAGDEKSYRSLTQSWFG
                     NSPLWALLQKLSEKKVTVVITTDHGTIRVKTPVKVVGDRETTTNLRYKVGRNLNYDRR
                     EVFEIREPKKAGLPSPNISSAYIFARNDSFFLYPNNYNYYHNYYLNTFQHGGISMEEM
                     ICPVVVLSPR"
     gene            complement(34915..35367)
                     /locus_tag="PKM27_07710"
     CDS             complement(34915..35367)
                     /locus_tag="PKM27_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07184.1"
                     /translation="MNRLSSGWTLVLRLFVPVFYVVFMGALTLATLSQGADLSPVFGT
                     WIYRLAMPGLVLAGILTLRFTVWKLLRLDASPDHFYITNYFRTYRYSLDSIESIRPFG
                     LFFLKFIKIKLKEKGSLGKELYILVERELWDQYADAHPQVKALMQSPG"
     gene            complement(35368..36213)
                     /gene="tatC"
                     /locus_tag="PKM27_07715"
     CDS             complement(35368..36213)
                     /gene="tatC"
                     /locus_tag="PKM27_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNR07185.1"
                     /translation="MKNKGDQEMSFLDHLEELRWHIIRALASILVFAIVVFLSKDFVW
                     QKIILAPIRKDFFTYRFFCDLSPKLCFGPDKIEIITRDIGEQFMMHMVSSFWLGVVVS
                     FPYILWEVWKFIKPGLYEKEQKTIRGVVGICSFLFFLGVAFGYFVVTPFGVTFLSQYE
                     LSNEIANTVTLSSYVNYLTMFVLPLGLVFELPVVVYFLAKVGLVTGEMLRTFRRHAIV
                     VVIIIAAVITPPDVASQILVSLPLIGLYEVSIWIAGRVNPLPKEEDEDKDKDEYDYDE
                     SKNKD"
     gene            36360..37574
                     /locus_tag="PKM27_07720"
     CDS             36360..37574
                     /locus_tag="PKM27_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR07186.1"
                     /translation="MKAFKTIAYLALVLFCLSSRELPGQDPAVYIPFDSCNLKEKNGV
                     LTGQSVGALVCECGAAGSALRWNGGFHFADWEGNALDNLFSGDFTVSFYFRPDAVTGT
                     MDILSKRENCGIDSLFAIRYLSDERTIVAELAQRVDQGGTLQGHLPENSCWYHIVFTR
                     SGRRLALFVNGEEKDSKTNNYIIDPRNNAKLALANSPCLGVTDRRFVGLFDELRLYKR
                     ALSRDEIRGLDLRPEHIANRDTVIFKGGSVQVRVNTDCSAQAQWSPVRGVADPLKRNP
                     LIVPDSTTTYVLRSDFGSCVVRDTFRVIVINEADLDCDQVNLPNAFTPNQDGLNDVYR
                     INNPYVIEDLHEFEIFDRWGEKVFSTNDSNGAWDGDFRGKPLNPGIFIYHLKYFCRGV
                     LRHKTGSLTLIR"
     gene            complement(38221..40818)
                     /locus_tag="PKM27_07725"
     CDS             complement(38221..40818)
                     /locus_tag="PKM27_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNR07187.1"
                     /translation="MKSKIAALILCLALLIRLQSQSFSPITLSYYLPELEYDPAVTTP
                     KAFLGYEVGERHASHDQLYYYMKTLASQSKRIKLVEYARSFEHRPLICLIVTSEKNQA
                     NLESLRQKHLELYDPAVSDRARLEETPLVLYQGYSIHGNEPSGANAALAMAYYLAAAR
                     GEEVEKLLDKTIILFDPCFNPDGVQRFSSWVNAHRSEAMVTDPSTREFNEPWPRGRTN
                     HYWFDLNRDWMPQQLPESRGRVNLYHQWKPNILTDHHEMGSNATFFFMPGEPTRVNPN
                     TPKLNQELTREIGRYHSESLNSIGSLYYTREGYDDYYYGKGSTYPDANGAVGILFEQA
                     SSRGHAQSTANGVLTFPFTIRNQVRAAFSTYKAAVEMHDKLLAYQRDFYKNALEDAAK
                     DPVKGYVFSAGRDRSRMNHFIDILLANQIKVHALKRSFKSFKPEEAYVVPAGQNQYRL
                     IKGMFEKRNRFEDSLFYDISAWSYLLAFNLEHLELGAADGISSLLGEAVNTLPVLKSQ
                     LTGSSDYAYALEWESYYAPAALYRLLQERVRVRLIHEDFEISTPAGNKKFRRGTLLIP
                     VQDQPIGKEALLAKLGELASRYKVEIHPLNTGLAVSGVDLGSPSSTVLSSPKVAMIVG
                     DGVDANDAGEVWHLLDQRMAMPLALADMAGLNNFNFQDYNTLILVDGQYNGMGAAATG
                     KLKTWAAAGGNIVAMGRAIGWLAAQGMSAAVVKPNQSSFEIKSGETPMRAYAEASADA
                     GTDLVSGAIFNTYFDETHPLSYGYSGKNLPLFRSNRIYLEIPKNAYAAPFRYTSDPLL
                     SGYITKTNLDQLKNSAAVLVSGTGAGKVICIPDNPNFRAFWYGTNKLFLNCIFFAPAI
                     SGMTVQRAE"
BASE COUNT         9844 a        10275 c        10806 g        10039 t
ORIGIN      
        1 cctgaccccg agccaggaca tcaccccgct gatggaaggc attttgaaat atgtgccggc
       61 ccctgtggtg gaagagggaa atttgcaaat gcagctgact tccatcgact attcctctta
      121 tcacgggaga atcgggatcg gcaaggtgag ccgtggaagc atcaaagaaa accagcccgt
      181 cagcctggtc caggccaatg ggaccgtgaa gaccaggatc aaagacctct ttgtttttga
      241 aggcctgggc aaaaagaaaa ccaatgaggt atttgccggc gacatctgtg cggtcgtggg
      301 cctggattct ttcaatatcg gcgacacgat tgccgactat gaaaacccgg aaggcctgcc
      361 caccatcacg gtggacgaac cgaccatgca catgactttc agcatcaaca actctccttt
      421 ttacgggcgg gacggcaaat acgtaacctc caggcattta aaagaaaggc tggccaagga
      481 aacagaaaaa aaccttgccc tgcgggtgga ggaatcgggc agtgcggaca ccatgctcgt
      541 gtatggacgc ggcattttgc atctgggaat cctgattgaa accatgagac gcgaaggata
      601 tgaactctcg gtcggacagc ccaaagtcat tgtaaaaatg attgacggca aaaggtatga
      661 gccctttgaa ctgctggtgg tcgatgtttt gcaggaatac agcggtaagg tgatcgacct
      721 ggtgacttca aaaaagggcg aaatgcacgt gatggagaca cgaaacgaca tccagcacct
      781 tgaatttgaa attccgtcga gagggcttat cggactgcgt tccaggatcc tggtgcaaac
      841 cgcaggcgag gccatcatgt cccaccggtt tacagagtat aaaccctgga aagggcctat
      901 gagcggaaga ataaacgggg tgttgattgc caaatccata gggcaggcta cggcctattc
      961 catagataaa ttacaggacc ggggttcctt ttttattgaa ccgggcgatg aggtatatac
     1021 cgggcagatc atcggcgaac ataccaaacc cggtgatctc gtcgtcaatg cctgtgaacc
     1081 taaaaaatta agcaacgtaa gggcttccgg ttctgacgag gccgcgcgca tcttcccgaa
     1141 gatcgacatg tccctggaag aatgtatgga atatattgaa gcggacgaat gcatcgaagt
     1201 cactccgaaa agcatccggc tgaggaaggt attcctggga gaggaagaaa ggaaaaaagc
     1261 ttcgaagtcc gcccagatcg agatggcgta actgttagca agttagtaag ttggcaagtt
     1321 tggcaagtta gcaagttggt agagcgtctt gcatctccta tagtccgggg atgtatttcc
     1381 atttaattct taattcctta attttttcat ttcattcaat gtatcccttc agtcggtaaa
     1441 cggccgtgcc cacccattcc ctgaaaatgg cctgccactc atcaaaacac cggagctggg
     1501 ggaaaaaata taaaggaaga gagacttttt cccattgctg gtaatcggcc ggatagggga
     1561 cgaatggaat gccctgtcga tggaaacagg ccgcggcccg gggcatatga agagctgaag
     1621 tgatcaggag gacaggagct ctagtcccgg ggccttcaat cgcgggggcc agggccgaaa
     1681 gggagtccaa cattcgtttc acattcctcg cgttttcaaa ggtgttcctg cttgtagatt
     1741 cggtcaggat ggtggaatcc ggccagttga tttcggttaa aaaggcctgg gaaaggaggc
     1801 tctcggctgc tttgtccctt tggagcccgc cttcgccccc gctcaaaatc agttttctga
     1861 cctttccgga acccatcagt tccatgcctt ccgtcaaccg gttgacatga tcgttaaact
     1921 ggatccggtc cggaaaccca ttgtggaggc cagcaaaccc cccgcccagg acgacggcgt
     1981 attcaaaggg gggggagatc gattcctgaa ggaggggttt ggcttcgatc ctccggtaaa
     2041 ccgtgttgat cattgcccgg tttccaaaga tcataaaaag gatcagcgaa aaaatacgca
     2101 tgcttttttt taaccgggcc ggtctgacca ggacagaaag gagcaataag gcaaggaccc
     2161 agtggaatgg gtgaagcaga aaggataagc cctttgaggc caggtaatac atagacgcct
     2221 tatttgagcg cggcccgttc tatcgcattg gtatgccagg acggggcccg gagtatcgca
     2281 gggatcactt cttcgggatg ggccaccacc gaccacatct ccgcggaatc cggattcata
     2341 aaccgctcct caatgcatct ctgcaactgg gcaagcaaag gatcgtaaaa accgacggtg
     2401 ttgaggatga cgatgggctt attgtaaagg cccagcctct tcagggaaat ggcttccatc
     2461 agttcctcca gggtgccgct gccgcccggc agggccacca gggcatccgc ccggtccata
     2521 aaactttttt tgcgctcatg catgtccccc acgatatgca attcggtgat gccccggtga
     2581 gcccattcca ccttctccat aaaatcagga ataatgccga tcacttttcc gccacgggcc
     2641 agtacccggt ctgcgatcct gcccatcaat cccgtggatc cgccgccgta gatcagcgtg
     2701 atgttttggg aagcaagcgc ctcccccaat ctttcagcag cttcaaaaaa ccggctgtca
     2761 acctgattgc tcgaagcgca ataaacacag acggaggata tggtattcat gggaacaagt
     2821 tagctagttt gtaagttagc aagtttgtaa gttagcaaga tggcaagttg gcaagtgagt
     2881 tgttaagcat aactctgacc tctaacctct tccgtctaac ctctgacctc cgtgatacct
     2941 ccccttgctg aacttggtcc cgttgaacac ttcggtacga cgaccttgtt tttgttcctc
     3001 cgggcttaat tggataaagt ccgagcattt ttcagaacag catcccctat gcttttccgc
     3061 gcaggatttg cattggataa aaaggaggtg gcaggcatcg ttggcgcaat tggtatggtc
     3121 gtcgcaggga ctgccgcatt gatgacagcg ggcgatgacc tcctcgctga tccgctcccc
     3181 aagccgttcg tcgaaaacaa aatttttccc caaaaaaagg ttgggaaggc ctttggcggc
     3241 cgcatcgtgg ctgtatttga tgatccctcc gtccagctgg tacacttgct taaagccgtg
     3301 atgtttcaga tacgcgctgg ctttttcaca ccggatcccc ccggtgcaat acatgatcac
     3361 cggctggtct tttttgtctt ccagcaggtc cttcacgatg ggcagggatt ccctgaaggt
     3421 gaccacctgg ggtaggaggg cattgcggaa atggcccacc tcgctttcat aatggttgcg
     3481 catatcgacg atcacgcttt ccgggcgaag ggccaggcgg ttgaaagctt ctgcatccag
     3541 gtgcacgccg ctgcgggtca cgtcaaaggc aggatcttca atgccgtccg ccacgatttt
     3601 cggcttcact ttgatgatca gggcaaagaa ggacctcgcc tggtgctcca cggcaaggtt
     3661 caggcggatg tcgcggaggg cttccaggtt ttgaaaaagg gattgcaggg cttccaggtt
     3721 tttttcggga agactaattt gtccattgat gccttcctgt gcgatataga tccttccctt
     3781 cacgtccagg cggtccaggt tttggtacaa ttcgtttctg aaggtctcgg gttgccggat
     3841 gaatacgtag cggtaaaagg acaaaacgat tctggattcc cgtatggagg cgtatttttc
     3901 tttgagttct ttccggttct gacggttgtg cagcggcttg gccataagga atgcaaagat
     3961 aaggggttag tgggttagtt aattagttgg caagtcggca agtcggattg caggtatttc
     4021 cgactttcag ctcataacaa aattaaaaag ggcttcgcgg atgcaaagcc cttttctttt
     4081 tttaggcctt ttgaaacttc ggcccaaggt ggggttcgac taataccccg gattctgggt
     4141 cagggttggc tggccgtcct tctggctgtt taatatctca tcctggggaa gaggataata
     4201 attgtttttg gcgccaaaaa cttttccggc catataaccg cgtttattaa acttcctgcc
     4261 ggagggttcg gtgccgggta cggcttcttc agccgcatat ttgttcaata ctttttcggc
     4321 aatgccccac cttacgaggt cgaaaaagcg atggccttcc atggcaaatt caaggcgctg
     4381 ttccatacga acggcatttc tggcaaaatc agcaccctga gctgaaaagg tacctgcttc
     4441 atagggctcg accttatagg ttaccgtacc gtcctgtttg gacccgtttt tagcccggtt
     4501 acggatgtga tttaccaggt tttcggcggc cgataaattt ccaagctcca cttcgcattc
     4561 agccagccaa aggaggacac tggacaattt gatcaggcgg taattattgt tcacgttccg
     4621 tctgctggta gagtgggtag tgctgccctc ttcggccaga taatacatcc attttttacc
     4681 cgtaaaaggg cccgcatagg cctgatccct gatccaggtg gagcctggca tgggtcccca
     4741 gtccaggaag ttgactcccc gtcgaccaac tgtccagtcg agacgcgggt ctacgggaac
     4801 ggatttatcc agggtaaacg ctgtgccggc actgatgcct tgatcgttcg gcaaatctac
     4861 cttgttgtag gtatccagtg agccatccgc cgcagcgccg ataaaaggaa ggcctttttc
     4921 atcagtcttg aacgcattga ccagattgtg ggaaggctga taaaacccgc agcatccacg
     4981 gccgggcgct gttgccgaat atggccagtt caggttgtcc cctgcatttc cattacctcc
     5041 atttgttccg tcatttacag aaaactgcac ttcaaatatg gattcggcat tattatttgt
     5101 tgccgttctg tagttgtcgt ggtaattggt catgagtttt ttaccggagt ttagaacgat
     5161 atcgtccagt aaggtttttg ctgctgccca ttttttctga aacaataagg ctttggcgag
     5221 ataagctttg gccgcccatt gtgtagccct gcctttttgc gcctgtaccg gcggcaggtt
     5281 tttggctgca aaatcaaaat cggcttcaat ctttggccag atatcttccg aattcgggat
     5341 tttggtggag ttggcatcgg tggccacata aatggtctca tctatataag gcaccttgtt
     5401 ccacattttt tttgcttcaa aatggtaatg ccctctcaga aaacgggctt ctgcaatggc
     5461 ctgcgttttt tccgccgggg tcatatcctt gacggcttcg ctgttggcca attgtatgac
     5521 atcgttgcat ctggctaccc cgtcatagga atgccaccat tttccaagga aataggtgtt
     5581 gtcggcgatc cagttgtatt gctcaataaa ggattgttcc ggttgatccc ccgcatccgt
     5641 gcctttgacg gcatcgtcac tgctgatccc gccaaaaaca taattggaaa cggtggactg
     5701 gcgtcccgta ttgccggcgc caaccccatc gagaagtgaa tacgcgccga tcagaagggc
     5761 atttaccccg ctcttattgc tgagctggtt caaggaggcc cggccctgcg gattcagatc
     5821 gaaaaaatct tcagaacagg aaaagtacat cccgatgaag accaggagaa gaaaatattt
     5881 tttcatttca attgaattaa atgtttaaat aattaaaaac caaggcggac accgaaaata
     5941 aatgatttgg cgaccgggta tgcaccttca tcgatcccca tatggatatc ctggttgtct
     6001 gtcccggagg tcctcaaatt gatatctggg tcaaggccgg tatatttggt cagagtgact
     6061 aaattctgtc cctggatata tacctgggca ttgctgatac ccgctttccg cgtaagggaa
     6121 ttgggaacag aataggtcaa ttgcacattc ctcaatcgca gaaaagaacc gtcttcaata
     6181 aaataagtcg agatctgctg gctggttgcg tccccggaat tcagccttgg aagtttggct
     6241 atatccccgg attttttcca ggaatcattc aataattctt tggagcggtt tccctgaaag
     6301 gtattaaaat cagtccaata gcgaacataa ttgaagattt catttccctg ggaaccctgg
     6361 gcaaaaaggg tcaggtccag gtctttatac cctacgttga cattgatgcc gtaagtaaag
     6421 tccggatgcg ggtccccgat aatggttctg tctgcggcgg tgatgacgtt gtcgccgttg
     6481 acatccctga acttaaaggt cccttccttg gtatacgtac cgaatttagg ggctgcgtca
     6541 gcttcagcct ggtctttgat cacgccatcg acgatatagc cgtaatagct ggcaatgggc
     6601 agcccggccc gtgtggcgga aatggccgga aggcgtgtcg cgaaaccgaa gacggtggca
     6661 ttgggatcgt tgttcaattt cagcacctca tttttataat gtgcaaaatt gactcctatc
     6721 aggtacctga agtttccaac attctcccgg aagtttatac ccaggtcaag cccccggttg
     6781 cggatttcac cgatattcgt atagggtacc gttccacttc ctgcggtgcg gggaggagct
     6841 atctgtgtaa gcatgtcgga ggtgatgcgg ttgtaccagt cgaaaaccac ttccagccgg
     6901 ctgtccagga gggcggcgtc aaaaccaata ttcgtagaag tattggtttc ccattttcca
     6961 tttgagtttc cgaactgaac caggtcaaat ccaccgacaa tggttgagcc ggtaccgttg
     7021 atgtcgtagg cattgtttat cggatcaggt gcgtataagg tataggcatt gtatacgttg
     7081 ggaatcaatt ggtttccggt tttcccccat ccggcgcgta ttttcaaatc attgacggcg
     7141 ccgatctgtt tcataaagcc caattcggtc agtcgcagac cggcagaaaa agcggggaag
     7201 atcgcccatc ggttgttggc cgaaaaacgg gaagatccgt ccctgcgcag ggtgaaatct
     7261 gcgaggaaaa gatttttata ggcatagttc acttttccaa actgggaaaa caaggcacta
     7321 agggtagcgc ctgctccgga attacttagc ccggcggacg accctgcatc caggtacctg
     7381 taatccaggt catcaaaagc aaaattgctt ctgcttgcga caaagcccac tgcatttgta
     7441 gtcacggctt ctgtgcccag gaggacatta aacgtatgat ctccgatcgt tttatcataa
     7501 ttcagggtat tgtaccaggt gagggaccgg tcggtattgt tgaggacgtt caggctgttt
     7561 gcatttctgg cttcagcagc ttcgatatcc cggtggaaaa attcggagcg attgaacaaa
     7621 ttatattcga tacccaggct ggtcctggct ttcaggttgt ccagtatact cagttctgcg
     7681 aagacgttcc cgaaaacatg tgaccctttg gtgatgttgt ctttttcgcg atacagcctg
     7741 gccagagggt tgggggcgtt gcccaggttc accccgcggc ttcccgcaaa accgttatag
     7801 ggggaggtgt tcgtgccgcc cagttctggc ggaccgccgg tgatatcaaa tacggggatg
     7861 atcgggtgta cccggatggc aaacatgatc ggattggatt cattgttgtt tgtgatcccc
     7921 tgcctttcat cataagaata ggtaaagttt tccccgaaag tcagtttgcc tttggtgaat
     7981 tcggtattgg ccctgaccga atagcggtca tagttggtgt atctcaaaat accatcctgt
     8041 ttgtaatagt tggcagaaat ggaatacctt ccgaatttag tgcctccgct tgctcccagt
     8101 tgatagttgg cgatgggagc cggatcaaaa atgacgtcct gccagtcggt gccttgttta
     8161 tttgccttgg tgatgttgaa cgcagtacgg cctaaatttg gatcggcaat attgttggta
     8221 tacgtatagt tggcaggcag ctccccatag atattggggt agatgtaatc ggcgatggtt
     8281 tgttcgccgt taggaccaaa tttatattgt gcagaaggag aggtcaccga agggtttttc
     8341 cctgctccga tatcggcaag ataaagatac tggcccagtt cctgcgtatt gagcagatcg
     8401 agggtcttac ctggtctctg ggttcccgta taaaaatcaa aggtgatcag gggatcgccc
     8461 accttccctt ttttggttgt gatgatcacc actccattgg ttgcacgcgc tccgtatatg
     8521 gacgcggcag aagcatcttt caggatctgc atggattcaa tatcgttggg attcagttgg
     8581 ttcagggtgc cttgcgtcgg cactccgtca atcacataca acggactgtt gttgttgatg
     8641 gaaccgaatc cgcggatacg caccatggtg cctcctcccg gagaattgtc gttgcccacg
     8701 ttgacccccg atgccctgcc ctgcaacatt tgtgtgacgc tggccgttgg cacagcggta
     8761 aggtctttgg aattgacgac ggtaacggca ccggtgatgt cttttttacg ttgagaggca
     8821 tatccggtca ctacgatctc ctccagggtg cttacatcgc tgggaagggt gatttccacg
     8881 gaattggtat tggtgacatc cacctcgcgt gtcagaaaac cgattgagga aatcaccagg
     8941 gatgttgtcc catccggcac ctcaagtgta aaattaccat ctatatcggt agaggttccc
     9001 gtggtcgttc ctttgaccat aaccgttgcg ccaatcaaac ccgaatggtc atcgcctgca
     9061 atgactttcc cgttgatggt gcgttgtgcc tggaccgtca gtccaagaaa cagaaacacg
     9121 attgggaata cccatcgtgg tttcaaattg attgaaatca atgtagtcat aagtatgaaa
     9181 tgagagtttt tttcagggtt ggagtatagt tcgtacgtca gggtaagccc gctaaaacca
     9241 tccagtcgta taagcctgaa aaggccaggg aatagcaaac aggatcatgg ccagggcaat
     9301 caaataatac cagaacagga tccggaaaga ttgcccgctg ctgttctttt tggctttaac
     9361 aaagcctatg gtaatcaggg ctacggccag caacatcatc agcggatgct cgacggcaaa
     9421 aaaacgcatg accttgtcct tcatcatcga aggtcctgct tcgatccgcg gcgtaaaaaa
     9481 gataaggatg cacccgatca gcaactggag atggcaaaga ttcagtgcaa ggaaggaagg
     9541 gccccgggtg tccttgttga gagtagcctg ttgtttccat ttttgccagg catcataaat
     9601 ggcccagagc agggcgaaca atacaagcca tctaactccg gaatggaggt gtttcaggat
     9661 agggtacata caatgagagt ttaccttcgt gaaggtatga caatcacggc gcaggaccca
     9721 aaaatttttt ttaatctgat tttattggga tctgatccat cgcatattcc gccagtgccg
     9781 tgatggtcag gctgggattc actccgatat tgaccggaat aatgcttcca tccaggatat
     9841 acatgccggg atacccatgc acccggaaag catcatccac caccccttca ttgacgctgg
     9901 cccccatcgg gcatccgccg aggaaatggg ccgtggtgga catattaaaa aacgtttcgg
     9961 tcacagcgct gacgggcaca gcttttgttt ttttagccag cctttgcata aaatcatatc
    10021 cggcaggtat aaataccggg atgggttttt catcaccgtc tgacaccagc ctcaggccgt
    10081 tcccggtcca ccgcatggcc agcgcatttt tgatatgttg catgaccagg atcaccaccc
    10141 ctctccggct gtcttgtccc cggattatcc atttgagggt atccagtggc cggagaaaaa
    10201 ttttcgccag gattttaata ctgcgcacga taggcgggcc accaccatgg gccgtgagaa
    10261 aggccagacg gaagagggag cccgatccat cggggaattt gcacaattct atttgcgtat
    10321 caggatcggc ctgaatgatc gaactgatcg ccaccccgtg atttaatttc cggtcggcgc
    10381 cgatcacccc gcaaatggat tccgaattgc ttaagatatg acgccccaac tgatcagaaa
    10441 ggcctggaag tgtcccatac ctgaatttct gccggaacaa aagttcgagg gtgccgagca
    10501 ctcccccgct gaggatgatg cccctcgcct tgaattcgcc attgatcttt tttttgaaag
    10561 gggacctcac ccggatcgta tagacggatt gttcctgatt ccattcgatc ctttccgcct
    10621 gggtcagggg taggatcgtg gctccgtatt tttcggcaaa ccagaggtaa tttttctcca
    10681 gggtgttttt tgcgttgtgc ctgcatccga ccatacagcc cgcgcattca atacagggat
    10741 ttctcaaagg gccttcccca ttgaaataag ggtcccttgg cgtagggtcc gaatcataat
    10801 aaacccccac atctactcct tgaaacgtgg attcccggcc caggtcccgg gcaatctccc
    10861 ggaggaggag gtcatccgga ttctgattcc gggcaggggt gacccccagc attcgacggg
    10921 cggttttgaa aaagggttcg agggccttct tccaatccct ggttttaagc gggatgtttt
    10981 cgtagaaagg atccggaggc tccagcaggg tattggcata gacgaggctg cctccgccta
    11041 cacccacgcc cgacacgaca aataattttt taaagacggt gagttttaaa aaaccggtcc
    11101 accggaaaaa gggcatccag aggtattttt tgaaattcca gctggacttt ggaaaatcct
    11161 catcccggta ccttctgcct ttttccagga ccagtacgga ataccctttc tgcgccagtc
    11221 gcatggcgga tacactgccc ccgaaacccg aaccgacgac gatgtaatca taaatccctt
    11281 cacccgccaa gcctgccttt ccattcatat ttccgcttaa aaatactaag caggacgatg
    11341 atcagcaggt aaaaaggata gaccagggca caggtaagaa aggagaacgg ggatatacgc
    11401 aaacggaaaa aaggggacaa ggctttaaaa tataaataat cgatcaggaa tttcccggcc
    11461 caaaaggata cggtaaacac ccaggcctgt ccgctccaca gggagtatgc caacattcca
    11521 accatcgtca tcgcgcagag gattggaata aacaaggcca gcttgatctt ccagtcctcc
    11581 agcgcatggt tttttgagcc ccaccgcagc cgctgctgaa tgaaggagtg gatgttttta
    11641 agcggccggg tgccgaccag cgccgagggt tcaaataaaa aaccaaccct ggatttatcc
    11701 atttttacgg ccgcctgcat caaaaaaaga tcatccccgg aagcaaaggg gttctccggg
    11761 tccgaggccc gcatgtagtc ataaaccgac cggtgagcgg ccagattggc gccgttggcc
    11821 agatgccacc cgcccgaata aatgccagcc tgggtcatgc ccattaaggc tatcccttcg
    11881 aaagcctgaa aatagtccag caaggtatgc gtctcagatc cgggaatata aaccgggccg
    11941 gtaaccatta ataaacctga gcccgcgatc cgggtacaaa tggtttccag ccaatggcgg
    12001 ccatagaaac agtcggcgtc agcctgcaca aggtattctc ctttggcggc cttcagtcct
    12061 tcggcaatcg cttgtttttt gccctctccc cggtttgata tgatcctgaa attcgttggc
    12121 aggccggcat ccgccagaaa tgccaaaccc tggtcggtgg aatgatcgtc gatcaatata
    12181 cattcccagg attcggaagg cagggattga ttccggagat tttcgaccag acccggaagg
    12241 ttttgggctt catttctaaa tggaatgagg accgaaaaaa aagccggggg ccggggaacg
    12301 attgctcttt gggccttttg ttcctccgcc tttttccagg aatagatgcc ggtaataatc
    12361 agggcgcaat aagcaaggaa aacaagcagg gcggggacaa acatattgtc cggtgaaatt
    12421 actaaaagag gaactcagaa gatggagggt tgaaggttga gggttgagga ttgaggattg
    12481 aggattgagg gctgatcact cacttcttcc caaacaaatc atcatactgt tttcccgcct
    12541 cggaggaaat ctccgtatag tccgtcggga tgccttcttt attcctgatt tcttccttga
    12601 gaatattgtc ggctttttta ttcatcagcg ccatggcaaa caggagggcg gctacgatgg
    12661 ggaaggcgac aaaggtgagg atgatggcaa tgacccctac cagcgtgttt ctttgcagca
    12721 gcaccaccag catcttgcca tagctgacga ccagtttgta attgatgatc agggcggcga
    12781 taaaaaggat gggcgcagcc caggccagga cgcggaaaat gcttttaaac agaaaaaaag
    12841 cggcccaaag gaccagggca aagaagacca ggcctacaat ggtttcggta gtggtataac
    12901 ggcggttttc cattttcgca aaaatttaaa aataaaacaa atttaagggc ggttctggtt
    12961 ctgattcagg ccaataatcg atcaacttca gccaatacct gatagaaatc gccggtttcc
    13021 gggatcagtt cacaattcag ccatcttggc aaactggtaa tcgtgcaatt ttcgatacca
    13081 tccgtccggc ttatccagca attcttcgtg ggtgcctatt tccttgatct gcccctggtc
    13141 catgacgatc acctggtcgg cgtggcggat cgtgctcagg cggtgggcga tgatgatgga
    13201 ggtccgtttt tcaatcagtt tttcaatggc atactggatg acagactccg tctccggatc
    13261 aatcgaggaa gtggcttcat ccaggaccag gatgtccggg tcaaagacca gggcccggat
    13321 aaaagagatg agttggcgct ggcccatgga taaattggat ccccgctcgg tcaccacaaa
    13381 atcatatccc ccgggaagtt tttcgataaa agcgtgcgcc ccgatctgcc gggctgcgcg
    13441 gatgacggtt tccgacggaa tgccgctctg cctgaaggtg atgttttcca agaccgtacc
    13501 tccgaataag aatacgtcct gcaggaccac gccgatccgg cgcctcaggg caaccagttc
    13561 gtactcattg atgttttttc catcaatgcg tatttcgcct ccctggatat cgtacaagcg
    13621 gttgaggaca ctgatgaggg tggttttacc tgaacccgtg ctgcctacaa tggcaagggt
    13681 gctgtgcgcg ggcacatgaa aactgatccc tttgaggact tcctgttcgc cgtcgtagct
    13741 aaaccttact ttatcaaaga ccaggtcccc ctgcaaacgc tggggtacaa ggattccttc
    13801 atttttaatt ttgctttgat tgtccagtac gccaaaaacc ctttcggcag ccaccagccc
    13861 catctgcagg gtgttgaact tgtcggccat gaaccggatc ggacggaaaa gcatggtgag
    13921 gtatatcgga aaggccacca gggcgcccag ggtcacctct ccttccaaaa cgccccggga
    13981 tccccaccag accatcaggg ccagggaagc tgcggagatc agctcaacaa caggaaaaaa
    14041 tacggcgtaa taaaatatgc cgtcgatatt cgcctgtgtg tattcgcggt tgattttacg
    14101 aaaggcttcc tgttcctgtt tttctgcgtt gaagatctgg atgatccgca taccggagat
    14161 ccgttcctgc agaaaggcat tcatgcgggc gatatgggtg cgtacctgct gaaatgaaac
    14221 ccggactttt tctttaaaaa gataacttgc gtacaagaga aaaggcaggg tggtcaggca
    14281 gatcaggctg agtttccagc tcaccacaaa catgatgatc agtacggcga tcagtcccag
    14341 gatatctgcc aggatggtga tcaccccttc ggaaaaaact tcattgatgg attcaatatc
    14401 gttgatggtc cgcgtggtgg aagtgccgat gggcgtcttg tcaaaatagg ccatccggag
    14461 gtccatgata tgattgaaga cccgggtcct caggtcacgg atgatggact ggcccagggt
    14521 gttggtccat aaaatgaaga tataccggag ccagacggtg gccagcaata ccaccatata
    14581 ccagaaaacc agcgggccga ggccgcccag ctcatccttc atcacatgat tgtccaccat
    14641 tacattgatc aggtggggac ggaggatgcc caggggagcc tggatgacgg ccaggacaag
    14701 ggtccagtaa aataatttct tatatgggat ggacaactgg attaccctcc aaagcaggga
    14761 ataattgaat tttttcttgg aatcagccat cgaatcagca aaagtaagga tttgagtggt
    14821 taaaggagga agggaggaat ggaggaaggg aggaagggag gggccagggt ctggcaaaga
    14881 atggaaatcc ttacgcggtt aaaggtgaaa ggtaaagggg aaagggagca tcagtacttt
    14941 ctggactggg tcaatgcggt ccacaggcag cttatgaaat ccggcttgtt ttccggaagt
    15001 cacggatcaa atcatgccct catcggcaaa gctgaaaaag ccttccctcg aaatgatcag
    15061 atggtcggcc aggcggatgt caagcagttc acctgcagct ttgagttttt tggtgatggc
    15121 ttcgtcctct ttgctgggcc tcagattacc ggaggggtgg ttatggctca cgatcagcga
    15181 gaccgcctgg tgttcgagtg ccttgccgaa gatgatgcgc ggatcggcgg aaaccgagga
    15241 gatgccgccc aggtggattt ggccgaggta taagacctgc agttggcggt tgaggcaggc
    15301 catccagaat tcttcctgtc tgctgtcttt taagtgagaa aagagcgaag cggcctcgga
    15361 actgctttgg atttttttcc ggtattcggg agcggcgccc tgtcttctcc ggcccagttc
    15421 gagagcggca tggatggcaa tcgcttttgc ggggccgatc ccttttattt ttttcagttc
    15481 ttgatggctc aggccagcca gttcgttgag gttatggcct atgcgccgca ggaggtcacg
    15541 ggaaatgtcg agggcgctgt gttcacgggt gccgcttccg atcaggatgg cgatcaattc
    15601 ggaatctgac agggcaaagg gccctttttg aaccagtttt tcgcggggct tgtcctggtc
    15661 cgcccagtat ttaatgctct ttttattttc atccatggct caaggcaaga tcgaggtcag
    15721 caatcaggtc gtccacatct tcaatgccga cgctgagtct gatcaacgaa tccgtaagcc
    15781 ctgtcctcaa ccgttcttca cggggtatgg atgcatgggt catggtggcc gggtgcccga
    15841 tgagggattc cactcctccc agggattcgg ccagagaaaa cagatgggtg ttgctcagga
    15901 cggtgttggc gtgttcgatg cggttgtcct tcaggtcaaa ggaaatcata gccccgaaat
    15961 cccgcatttg ttttttagcg gtcccgtgat tggggtggct tgcgaatccg ggataaaaaa
    16021 ccttgtccac tttgggatgc gcgtccaggt ggtgggctat gcgggcggcg ttttggcaag
    16081 cccgctgcac ccgcacatgc atggttttga tgccgcgcaa gaccaggaaa caatccatgg
    16141 gtcccggcac ggccccggtg gcattctgta taaaatgcag ggagtccgca agggccttat
    16201 cttttacaat cagggcgccg tgcacgacgt ccgaatgtcc gctgatgtat ttggttgccg
    16261 aatgaaggac gatgtcggcg ccataatcca gggggttttg aaggtaaggg gaagcaaagg
    16321 tattgtcgac gcataccagg atgccgtgct gtttggccag cccgcagacc agttccaaat
    16381 ctatgatatt gagcatagga ttggtggggg tttccaccca aagcagcctt acccccggcc
    16441 ggatatgttc ccgggccact tcgggatcgg agagattgac aaagcgcgac tgaatgccga
    16501 atttttcgta cacccgcctc atctgccgga aggacccccc gtaaaggtca ttggtcgaaa
    16561 ggatcaggtc gcccgggttg agcagtttca tcaccgcatc ctgggcagcc aggccgctgg
    16621 cgaaacaaat gccgtaatcc ccgttttcca gggcggccag gttggcttcg agcacactgc
    16681 gggtcggatt ctgggtacgc gcgtattcat accctttatg ttttcccggc gcttcctgca
    16741 cataggtgga ggtctggtaa atcggggtca tgatggctcc cgtcgtggga tcgggttcaa
    16801 tgccggcatg aatcagcttg gtggcgaatt tcatgggatg aaggtaagga aaagtagtca
    16861 gcggtcagca gtcagtagtc agtagcgagt tgtctcgttt ctcgcatctg gcttctcgca
    16921 tctcgcatct cgcatctcgt acctctgacc tctgacctct gaccttctac actaccggca
    16981 aactataccc attatgatac tccggcatga ggtattcttt attgactgct tcatccgtaa
    17041 aacgttgctg gttggcgttc cagtccagtt ttttgccgct gcggaaagcg atattcccca
    17101 tctgacagac tttggcgacc agggagccgg cctggatggg ggcgttgagg tcttccatct
    17161 tccgggaacg gacgacgcca aagaaatttt ccatgtgttt ttccaccccg ttgtcttcac
    17221 gcttttgcag cgggatcagg atcttgtttt tctccctttt ttcttcgatc acttcccaac
    17281 ctccgcggtc gaggacgagg gtcccgttgt tgccgatgaa ggatatgccg tgatcccggc
    17341 cgtagggccc gttgtcgatg ctcatggcat gttcccaact gaggataaaa tgatcaaact
    17401 catagatggt gtgttgggag tcgggggttt catgactgag ttcgggatag gcaaatttcc
    17461 cgcccaggga ggccacggtt ttgggcatga tggccttcat tccgaacaaa gcgtaatcga
    17521 tgagatgcac gccccaatcg cacatcaggc caccggcata ggcccagaac caccggaaat
    17581 taaaatgaaa atggctggtg ttgaaaggcc ggtgcagggc agggccttgc cacatatcgt
    17641 aatccacgcc ggcagggacc ggggcgtcgg gcacaaccgg agccggtttc atccagccct
    17701 ggtaagccca ggctttggcg gttcggatat ttccaagcct gccggaatgc acaaattcga
    17761 tggcgtcccg gaaatgtttc tggctgcgtt gccattggcc gacctggatg gcccggttgt
    17821 attttttctg ggcttgttcc atggccaggc tttccccgat ggaattggcg atgggctttt
    17881 ccacatagac atccttgccg gcctggcagc cgtggatggt gatcagggga tgccagtggt
    17941 ccggggtgcc gatgatgatg acgtccaggt ctttcatctc cagcattttc cggtaatctt
    18001 tgaacgtttt gattttggcg gcatcgacct tcaggtcttt gagttcggcc attcttttat
    18061 ccaggacgga ctggtcgatg tcgcagagag cgaccaattg ggcgtttggc tgggtcaggg
    18121 ccgcccgggt attggcccag cccataccat tgatgccgat ggtgccaaat cgaagcgaat
    18181 cggacgggga ggccttgcgc ttatagatca aaaaaggagc ggtgttgcct tgggcgagca
    18241 ggctggtccc cagtgcggcc atggaggctt gtttcaggaa ttttctgcgt tttgacatgt
    18301 aggttatatg aaggatgacc ggataaaaaa atgaaaaaaa acccggtaca gcggaaccgg
    18361 gggtaaattt ttgagcggaa aacgggattc gaacccgcga cccttagctt gggaagctaa
    18421 tgctctacca actgagctac ttccgcttgt aaatgggcac taaattaaga aataaattcg
    18481 aatggcgagg tcactacggt gactcgccat cgcgagcata acctcgcgac ccgcgaccct
    18541 tagcttggga agctaatgcc tgcctgactg cctggttaaa agtttcagat aagttggtgg
    18601 caggcaggct ctaccaactg agctacttcc gcttgtaaat ggacactaaa ttaagaaata
    18661 aattcgaatg gcgaggtccc cacggcgact cgccatcgcg agcatagcct cgcgaccctc
    18721 gaccccttgc ttgatgaact aatccctgcc cgatgtccga gtcaaaagtt atttcaaagt
    18781 ccagtagcag gcaggctctg attttctaac tagccaacta gccaactagc caacttacta
    18841 acttactaac taactcaact tttcaccccc tccaattgtt tccatcttgt atttttgtaa
    18901 aatctaaatt ctaccaggaa tggatacagc agtaaaaagc ccggtcatgc tctacacgga
    18961 acagacgccg aacccggaaa cccttaagtt tgtgctcaac cggatcattt acaaagggat
    19021 agccgatttt aagcaggcgg acctggcttt ggattggtcg ccctttgcca gcgccctctt
    19081 tgagtttcct tttgtcaaag gggtctatgt atgcaacaat tttgtcacca tcaccaagga
    19141 aaacacggtc gattggaacg acatcatgct ggagctcaag gaatacatca aagcctatgt
    19201 ggccgaaaac aaaccggtga tccgcgaagg gtaccaggaa ttcatggaac agcaggaaag
    19261 ggaaaattcc accattgaat acagcgaagc cgatgccgaa ctggtaaaaa gaatcaaaga
    19321 cctgatcgaa acctatgtaa aacccgccgt ggaaatggac gggggcaaca tcgaattcaa
    19381 agcttacgat caggggaccg tatatgtgat catgcagggc gcctgcagcg gttgtccttc
    19441 ctccaccgtg accctcaaag ccggtattga aggcatgctt aaaagaatga tccctgaagt
    19501 aaaagaggtg gtttccgaaa tgggttagcg ggtggatgca gctttattgc ctgaagtaga
    19561 taaactggtg cgggaaacgg gggagtggat cctttcccag caagcggttt ttacggactc
    19621 cgacatcgag gagaaaagcc tcaacaacct cgtatcctat gtggacaaga cggcggaaaa
    19681 aaaactggtg gaaggacttc gggtcctatg gccccaggcg gcttttctga ccgaagaagg
    19741 cacggtgcac tatcagccgg ccgaatggga atggatcatt gacccactcg acgggaccac
    19801 caattttctg caccagatcc cggtttattg catcagcgtc gccctccgcc ggaaccagga
    19861 aattgtcctg ggggtgatct acgaccccaa tcgcagggaa tcctttaccg ccttgtcggg
    19921 aaggggagcc catctgaatg gcaaaccgat ccgggtaagt acgacccctt cattgcaatc
    19981 agccctgatc gccacaggtt ttccccatcg caattttgaa ggcatggacg cctatatccg
    20041 gtcgctggaa tatttttttt accgcacccg gggactccgg agaatgggcg ccgcagccat
    20101 cgacctggcc tatatcgcct gcgggcggat ggacggattt ttcgaactca aactcaatcc
    20161 ctgggatatc gccgccgggg tactcctgat ccgcgaagcg gggggcatga tcagcgactt
    20221 cggaggaagc gatgattttt tattcaaatc cgaactgctg gccggaaatc ccgcggtata
    20281 cctggaaatg aaagaagtgc tggccaaata tttctgaaag gccccgcctt cctggtctaa
    20341 tttttttcca tattaatcga atccggtacg gagagccgcg gcaaacccgt aagtttatac
    20401 ttttacgccg tcaatacctg cgaatgttgt acgaaaatgt cagaatggcg ctgtcttcca
    20461 tcaaagccaa cctcacccgg gcgatcctta ccctcatgat catcgcggtg ggcatcatct
    20521 gcctggtagg tatcctgacc gccatcgatt cgctgatttt ttcattaagc gataattttt
    20581 cgagcctggg cgccaattcc ttcagcatca gtccttccgg ggaagccatg caagccaggc
    20641 gggaaggccg gatggaaaaa agaggggaag aaatcacctt ccagcaggct atggatttta
    20701 aggaaaaact caacatcgac ggccgggtga cggttcattt caacgggacg agcagggcgg
    20761 ctctgaaatt tgaaaacgaa aaaaccaatc ccaccatcac cgtcatcggg gtggatgaaa
    20821 attatctcac cgtaaacgga tatgatatcg aattcgggcg cgggtttacc aatctcgaga
    20881 tccaaaacgg agcgccccgg gtcattctgg ggaccgaact cgtgcagacc ttgtttaaag
    20941 ggcggggccc tgccgccctc aaccagacca ttcaggtggg caatatccgg gtgcaggtgg
    21001 tgggcgtgct caagtccaaa gggtcgtccc tcaattcctc cagcgaccgc atcgtgctga
    21061 tgcccctgct gcaggccaaa caatactacg catctccaaa tgagaacttc aatattgccg
    21121 tccaggtcaa taaggtcagc gggatggatg acgccatcaa cgaagccacc gggaccatgc
    21181 gcgtggtccg gggactccgg gtaaatgaaa ccaacgattt tgaaattcag aaaagcgaag
    21241 gcctcgtaaa catcatcaag gaaaacacgg tgaaaatccg actggctacc atcggcattg
    21301 gcctgatcac cctgctcggg gcagccatcg gactgatgaa tatcatgctg gtctccgtga
    21361 cggaacggac ccgggaaatc gggatcagca aggccatcgg ggctaccagc cgaaatatta
    21421 tggtccagtt tctgaccgaa gccatcctga tcagcctgat gggcggcctg gtcggcatca
    21481 ttctgggtat tttcgtggga aatattgtca ccctgcttat cggtggaaaa ttcctcgtac
    21541 cctggaattg gatttccctg ggggtctttg tttgtattgt agtcggggtt ttctccggcc
    21601 tttacccggc gcttaaagcg gccaggctcg acccgatcga atccctgcgc tacgaatagg
    21661 tcaggaacca aaccggattt ttcgtcacct ttcgatcact tcatcccttc ccaccccgac
    21721 ggatatcagg ctgaatttcc gccgcaataa attttccagg gattcaatat agtgcctggc
    21781 tgccccgggg agctggtcaa aagtctgggc ttcatgcagg ctggtgcccc agcctggaaa
    21841 ggaaaggtat tgaggctggt aaccggaatg gtcaaggtcg aaggggatgg ttttcgtttt
    21901 ttctccctgt ccgtcgaggt aactgtcgca agcccggata gtatcaaaat cgttcaggac
    21961 atcgatcttg gtcatgatca gttcgttcac cccgttgatc ataatggaat aattgagttg
    22021 caccaggtcg atccatccgc agcgcctcgg gcgcccggtg gtggacccga attcatggcc
    22081 ggctttacgc agctgatccc cggtttcgtc aaacaattcc gtaggaaaag gtccgcctcc
    22141 cacacgggtg cagtaggctt tggtgatgcc gatgaccttc ccgattttac cgggcggtac
    22201 accgaccccg gtgcataccc ccgcggagat ggtgttggag gaagtgacat aaggataagt
    22261 gccgaagtcg atgtccagca tcgaaccctg ggcgccttcg gccaggacat tccggccttc
    22321 ccgcaaggcc tggtcgatat aatattcgac atccactttc cggaatgccc tcaggttttc
    22381 gaggcttgcg aaccattttt cttcggcttt ctccaggtca tattccactt cggggtaaat
    22441 gcgggccagg tcgaggtgtt tttccttcag gacctggtat tttttgtcaa aaccggcaag
    22501 ttccaggtct ccgacgcgga gtccgttgcg acctgtttta tccatatagg tcgggccgat
    22561 gcctttgagg gtggagccta ttttgttcag ccctttggat tgttcggaag cgatatccag
    22621 aagctggtgg gaaggcagga tcaggtgggc cttgcgtgcg atgatgagtt tgtgggcgat
    22681 ctcgtatccc aggcctttta atttgtccgt ctctttctca aaggttaccg gatcgatcac
    22741 gaccccgttg ccaatgagat tgtcaatgcc ctcgtgaagg atagccgacg gaatggtgtg
    22801 aagcacgatc ttctgatcag cggcataaag ggtgtggccg gcattgggcc ctccctggaa
    22861 tcggcagacc acctggtaat ctttacaaac cttgtctatg attttaccct taccctcgtc
    22921 tccccactgc agtcccaata atacatcgac gcccatttgt ttttattcaa aagtgaaggg
    22981 caaatatagg gaaacggaaa ttgaggttga ggttaaggtt aaggttgagg ttgaggttga
    23041 ggttgaggtt aaggttgagg ttgaggttga ggttgagtca agaatacacc aatcccaaag
    23101 acagttttac aattccacaa atccacaatt caacatttcc gcctctaaac aaccacaggc
    23161 tccattttcg tcctcagcaa ctgcccgctt tttttccttt cacaatccac ctggcaggtc
    23221 ccgtaaaggt tgagggagtg gtgttcgatc ttaaaatgca gcaactccgc catagtggtt
    23281 ttgatctgct gcacccgcgg atcgcaaaat tcaagcacct tgccgcaatc caggcagatc
    23341 aggtgatcgt gttgtttata accgaacgat ttttcatact gggcgaggtt tttgccaaac
    23401 tggtgttttt tcaccatttc acaattgacc agcaattcca gggtgttgta aaccgtagcc
    23461 cggctgaccc ggtaattttt atttttcatc tcgatgtaga gcgcctcggc gtcaaaatga
    23521 tccgaccggt tgtaaatctc ttcgaggatg gcataccgct ccggggtctt tctgaacccg
    23581 cctttgtcaa gatgggccga aaatatattt tttacctgtt caaaattcat aattcaaaat
    23641 tcattcaatc ctttccactg tagaaatcgc atcgagcttt gaaaggctgg acaccgcttt
    23701 ctgcagttgg tttttatttt ttaccatgag gctgatcttt ccctcaaaat acccttcctt
    23761 gccttctatg ctgaaggaac ggatgttgag attaaggtca ttggatatcg ccttgcttat
    23821 tttttcgatt acgcccggcc cgtcatcgat gccggtgatc atcaatttgg caatgaattc
    23881 gctgtcggaa tagtcgctcc attcagcctt gactacccgg taaccatagt tggccagcag
    23941 gtgggtggaa ttcgggcagt tggaccggtg gatctttaag ccggaggagg aggtcaggta
    24001 accgaatatg tcgtcaccgg gcagggggtt gcagcaactg gcgaaactgt attcatactg
    24061 ttccgccggc tcgccgttga tatacagttt ggtcttgccg gtggaggtct ttgccggtgc
    24121 gggagcctgg gcaggcccca cttccggatg agcctgggtg gcgaccggtt cggcttcagc
    24181 ctggttgaat aaaagtttgc cgttttcgac cggcacctgg ttcaattccg atatgtccac
    24241 ttccccggaa gcaatggaaa aataaagatc catccgggtt ttatactgat agtgcctgac
    24301 cagggcatcc aggttttctt cgaggtccag cttgaggttt ttgaatttcc tttccagggc
    24361 ctcgcgtccc aggtccgccc tttctttccg ttcttccttg agggcctggc ggattttggc
    24421 tttggctttg ccggtcacca ccagtttcag ccagccttcg gaaggttttt gtttgttgtt
    24481 ggtgacgatc tgcacctggt cgccgtgttc aaggtggtag cccattggca ccagcttgtt
    24541 attcaccttc aaagcggtac aatggtagcc gagatcggta tggatgctga aggcaaaatc
    24601 cagggccgta gcccctttag gcaggatttt gacgtcccct tttggggtgt atacgtatat
    24661 ttcttcgctg aaaagattgg atttgaattc attcaggaat tcaatggcat cggcatggtt
    24721 ggattccagc acttcccgga tgttgtccag ccagccgtcg taaagtttct gctggttgga
    24781 tatgcctttg tatttccaat gggcggcaaa gccccgttcg gcgatctcat ccatgcgcgc
    24841 agaacggatc tggacttcca cataccgtcc tcccgggccg atcaccgtgg tatggaggga
    24901 ttcgtaacca ttggctttgg gcgtggtgat ccagtctttg agcctttccg ggatgggcgt
    24961 gtgcacgtcg gtcacgatgg aatagatctg ccagcacagc gagcgttcca ttttagccgg
    25021 gacgtccacg atgatgcgga cggcgaaaag atcgtagatc tcttcgaaag gaattttttt
    25081 cttgcggatt ttattgagga tgctgctgat ggatttgggc ctcccaagga ttttatacgg
    25141 cacgttcagc tggtcgagtt ccacccggag gggcttgata aaccgttcga tgtactcgtt
    25201 ccgggcgctt tcggaatcct ggagtttgcg cgcgatttct tcgtaggctt cgggttcctg
    25261 gatcttcagg cagatgtcct ggaattcgga tttgatgttg tagaaaccga gccggtgggc
    25321 gaggggcgcg tagatgtatt ccgtctccgc ggcaatctta agctgcttgt gccggggcat
    25381 ggcgctgatc gtgcgcaggt tgtgcaggcg gtcggccatt tttatgagga cgacgcgcac
    25441 atccgtgatc agggtgctga gcactttttt ataattctcc gcctgggcgg tctccaggtt
    25501 gtaaagcccg tccagtttgg tgagcccgtc cacgatcatc ccgattttgg gcccgaactc
    25561 cttgttgatc tcctccaggg tgactggggt atcctcaacc acatcgtgaa gcagggcgct
    25621 gatcacggcg gtcggaccga ggccgatttc ttccacacaa atgcgggcca cttcgatggg
    25681 atggaggaca tagggttcgc cggctttgcg cctttgcgaa cggtgggcca ccaccgccat
    25741 ttcgtaggct ttgcggatat tgattttatc cgtttcttcg agttctgatt taaacgtttt
    25801 taacagtcgc cggtaagcgg attggatgag gtggaggtcc tgttcatcct ccacatgaat
    25861 gctggccgta agcgttgttt ccggggacat atgactgctt aaagataaac gttttaaaag
    25921 tttattcgtt cacttaaaat cgtctacctt tgcgctccca atttttgcgg gtatggcgaa
    25981 attggtagac gcgccagact taggatctgg tgccgagagg catgggggtt cgagtccctc
    26041 tacccgcacc gggatttcta actaaaccgc attttaatgt cagtcagcac gcagaccacc
    26101 ggtcaactcc aggcaactct taccgttcat atcccggtgg atgaatacca ggccaccctt
    26161 tccaaagagc ttcagaatta caaaaacaaa gcgcagctca agggattccg gaaaggcaag
    26221 acgccggtcg acttgatcaa aaaactatac ggcaatgcca tcctgaaaga agtggtcgaa
    26281 aacagcctgc agaaacaatt gtacgaccat atccgtcaaa acaacatcca tttgctcggt
    26341 cagccgctga tgaacgcgga acaggccgaa atctactata acatccagga gccttccgat
    26401 tataccttcc tttttgatat cgggtatgca ccggattttg aaatccaggg ccttgaccat
    26461 gaattcggtt tttataaaat cgtacccagt caggaatcgg tcgacaagga atttgaaaac
    26521 ctgcgcaccc gcatgggcga actaaaaacc gttgaccttc ccgttgagga aaatcacctg
    26581 gtggatttta cggcagcaga actggatgga accgaactga aagaaggagg agtacagacc
    26641 gaattcagcc tttggatctc ccaggtggac ggccccgcca aaatttcgat tcttggtaaa
    26701 caaaaagggg atgaaataga ccttgacctc cgggaactgc ataccggcaa ggatgaggaa
    26761 ttcatccgga aaagatacct caccagcctc gaagagggac aacaggtccc ctcccgtttc
    26821 cggctgacgg taaaagacat ccgggccaat gaacccgcag tcacggacca ggcctttttc
    26881 gataaggctt tcgggccgga taaggtcaaa gacctcgatg aggccaaatc cttcatccag
    26941 ggtgtcctgg caaaccaata cgtttcaagg tcggaagccc ttctgttccg ggacatacag
    27001 gaccatcttc tcaaaaccaa taaacaggac ttccctgacg ctttcctgaa acgctggctt
    27061 aaatcgcaaa acccgaaatt gaccgacgaa ggcatcgaag ccgaatatga tgactttgcc
    27121 cagaacttgc tgtggaccat catccgggac aaactgatca aaagatttga cattaccgtc
    27181 agcgccgaag agatcaaagc ccgtttccgg caacaggtcc tcaactattt cggcggcatg
    27241 gccctgggtg atacgtcctt cctggaaccg gtgatccagc gtatgatgca aagggaggaa
    27301 gaggtggaaa aacaacacga cgagatcatg agtgacaagg tatttgattc cctgcaggcg
    27361 acggtcaccc ttgtcgaaaa agaaatctcc gaggcggatt ttgaagtgct gatggaacag
    27421 gcccggcagg aaacggcggc ccgccgtgcc aaagcggcaa aagcgcttgc cggccatgat
    27481 cacgaccacg accatgatca cgaccatgat cacgatcatg accattccgg tcacagccat
    27541 taaactttaa ttcattatta ttgttcctgt atttttgagc gacaaataca aacaatgaaa
    27601 gaaaatcagg aattcagaaa atacgcgacc gggcatctgg gcatgagcgg catgcacctg
    27661 gatcgttaca tgagccaggt gaccgtcccg gacatcagtg gttttacgcc gacggtcatc
    27721 gaagagcggt ccctgaacgt agtcgggatg gatgtttttt cccggctgat gatggaccgc
    27781 atcattttta tgggggtgcc ggtgaacgat tatgtggcca atgtaatcca ggcccaattg
    27841 ttgtttctgg aatccatgga ccccaagcgg gatatccaga tttttataaa cagtcccgga
    27901 ggatccgtga tcgacgggat ggggatttac gacaccatgc aatacgtttc tccggatatt
    27961 ggcaccattt gcacgggcct tgcggcttcg atgggcgccg tattgctggc cgcggggacc
    28021 aaaggaaaac gtacctgcct tcagcacagc cgggtgatga tccaccagcc ctccgggggc
    28081 atgcagggcg ccttcaagga tatggagatt tcctataaac tcatcaaaac catgaaggga
    28141 gaactttatt ccatcctttc acaccatacc ggcaaaagcg tggaggtcat tgaaattgac
    28201 tgtgaccggg acaactggat gaccgccctg gaagcaaaag aatacggcct tgtggatgaa
    28261 gtgctcaacc gggacaaccc gcggaaaaaa gtataaacct gccttttaat acaaccctgc
    28321 ctgatggcga aaaacagtga aagtcaggcg caatgcgcct tttgcggcaa acacaaaaac
    28381 gaagtggcca tcctggtggc gggcctcgac gcgcacattt gtgaaatgtg catcgagcag
    28441 gctcaccaca tcatccggga agaatttgat accaaaaaga aaaaatccaa gtcttacctc
    28501 attcccaagg accttacccc tgccaagatc aaagcccatc tggatgaata catcatcggg
    28561 caggacgaag ctaaaaaatg cctggccgtg gcggtttata atcactacaa gcgcctgcag
    28621 atcggctctt ccggggatga cgtggaaatc gaaaaatcca atgtgatctt tattggtcag
    28681 accggaaccg gtaaaaccct gatggccagg accattgcca aaatcctcaa cgtgcccttt
    28741 tccattgtgg acgcgacggt ctttacagag gccggttacg tgggcgagga tgtcgaaagc
    28801 atgctcagcc ggctcctcca ggtttgtgat tacgatgtgt ctgccgccca aaagggcatc
    28861 atctacatcg atgaaataga taagatcgcc cgtaaatcag acaatccctc cattacccgg
    28921 gacgtgtcgg gagaaggggt gcagcaggcc ctccttaaaa tgttggaggg agccgaagtc
    28981 agcgtccccc cccagggtgg tcgcaaacat ccggaacaaa aactggtgac ggtcaatacc
    29041 caaaacatcc ttttcatctg cgggggcgct tttgacggca tcgataaaat cattgccaaa
    29101 aggatcaata cccaggtgat cggctacggc gccaaggaca aggaatgggt ggacaaggaa
    29161 aacctgctca gttatatcac ccaccaggac atcaaggcct ttggccttat ccccgaactg
    29221 gttggccggt tgcctgtact cgcccacctc gatcccctca acaaggaaac cctggtccgg
    29281 atcctcaccg agcccaaaaa cgccctggtc aaacagtata aaaaaatctt ccagcaggaa
    29341 caggtaaaac tggaatttac ccctgaagcc cttgatttta tcgcttcaaa agccctggaa
    29401 ttcaaactgg gggcccgggg gctccgctcc atgctcgagg ccattctcat ggacgatatg
    29461 tttgaaatgc catcctccaa aaacaataag ggggaattga cagtggatct gttttttgtg
    29521 caatccaagc tcgaaaaatc aaaattatcc aagcttaaag cagcttaagg caagcccatc
    29581 accccttctt acaggaatct tgacctgatc ggtcggggct ttttattttt gcgcatgagg
    29641 tatttcttcg aatgttcctt cgacggcact ccttttcacg gctggcaaag gcaggcgggc
    29701 caggtttctg tccaggagat catggaaaat accctgaaga tcatcctgcg ggtccccgga
    29761 ctggaaatca ccggttgcgg acggaccgac agcggggtgc atgcggaaca gtttttcgcc
    29821 cattttgacc acggggcaga ccttcctccc ggcctggaat accattggaa tgccatcctt
    29881 cccagggaaa tcgcggtgca tcgggtctgg cgggtcgggg acggctcgca tgcgcgttac
    29941 gatgccgtct ccagaaccta ccaataccat atccaccggt ccaaatcccc cttcctggtc
    30001 aatcgttcct tctgttaccg gcaatacgac cggctggacc ttgatctcat acagcaggct
    30061 gcaagtcttc tgcttcagta ctccgaattt ctcccttttt gtcgcagtca cagcagcgcc
    30121 agtcattacg gcgtgcgttt gcaacaggcc gaatggaagg aggagaaggg ccctgcgctt
    30181 gttttgacct tgactgccaa ccgttttctc aggggcatgg ttcgactgat caccggcatg
    30241 tgcctgaatg tcggcacagg cgccgtttcc attgaagaag taaaacacag cctggacacc
    30301 cagacccttc tgcccaaaag ttttagtgtg ccggcgcatg gattgtattt gacagcggtc
    30361 agctatatgt agaagctgga ggttagaagc tcgaagctcg aagctagggc gacttggcct
    30421 ggcagttccg tgaggtttag aagaccctcc ccagctccca gccacgagca tcgagccacg
    30481 agcatccagc cacgagcatc cagctcccag cttctaactc ctagcttcca attcccagct
    30541 cccagcttct atctcaggat aaagtggaaa tcccgccatc atttcattga cctttttctt
    30601 aatgccaagg atgaggtctt ccttatccgg atgggtgagg atttcttcga tccagtctac
    30661 gatctggatg cattcctctt ctttcaggcc gcgggtggtg atggccgggg tccctagccg
    30721 gatgccggaa gtaaccgccg ggcttttttc gtcgaagggg accatgtttt tattgacggt
    30781 gatctcggct tttcccaggg cctcctccgc ttcttttccg gatatgtttt tattgctgag
    30841 gttgatcagc ataaggtggt tgtcggtacc cccggaaatg atctggtaat tcagttcgtt
    30901 gaaacgccgg gccatggcct gggcgttttt tttgacctgc acggcatagt ctttaaaact
    30961 ttcatccagg gcctcctgaa aggcgatggc cttggcggca atgatgtgtt ccagcgggcc
    31021 gccctgcatt cccgggaaga ccccgctgtc caggatggag gacattttta tcttgttgcc
    31081 tttggcggtg gtccggcccc agggattgtc aaaattttta cccatcataa tcacccctcc
    31141 ccgcgggccc ctcagggttt tgtgcgtagt ggaagttacg atatggcagt ggggcagggg
    31201 atcctggagc agtcctttgg caatcagacc cgcgggatga gcgatgtcgg ccatgagcag
    31261 ggccccgatg gagtcggcga tttcccggaa ccgggcataa tcccaatccc tggaataggc
    31321 gctcgctccg cagatgatca gtttaggtct gtgttgaagg gctgtttgtt ccaccttatc
    31381 catgtcgatt cgacccgttt cagcctccac tccgtaaaag accggcttaa acgtcttgcc
    31441 gctgtaattg accggcgaac catgggtgag atggccgcca tgagaaaggt taaatcccag
    31501 gatggtatcc ccgggctgaa ggcaggccag gaaaacggcg ctgttggcct gggcgcctga
    31561 atgaggctga acgttggcat agctggcgcc aaaaagggtt ttaaggcgtt cgatggccag
    31621 ggtctcgatc tcgtccacca cctcgcagcc tccgtaatac cttttcccgg gatatccctc
    31681 ggcgtattta ttgttgaggc aggtccccat cgcccggatc gtattgtagc tcgcaaaatt
    31741 ttcactggcg atgagttcta taccattgcg ttgcctcctg agttcttttt cgatcaggga
    31801 gaaaaccggg tctgtcatga ttttttattt aaagatggat gcgaagatag gaagtttaga
    31861 agttagtgat cagccgtata ccttccgatg catcggaacg aaggtaatta atccattatc
    31921 aggaatcaga agtcagaaag gtgggaatca tcgggttttc tatatttaat tattgataat
    31981 cagttcattg attcatattc attaacctat taataaacag attacggaca aaaaaaagaa
    32041 tacatgaata ttttaatcca ttatccggtg accctcaatc ctcacccctc aatcctcacc
    32101 cctcacccct cactcctcac ccctcactcc tcactcctca cccctgatac accataaagt
    32161 acttcgtttc ctgctgcgac atttgctgga aaccttcgaa tgcggaaagc gggatcacct
    32221 cctgcttttt ggtcaggatc atgatctcct tgatgtttaa tttgtaattg gaattggatt
    32281 ccttgccctg gaagatcatc ccgccgggat ggaggttccg gatgtttttt ttgacggccg
    32341 gactgaaagg tttgtcggac agcatgcatt taaacaggcg tcggtggatc aggcctccgg
    32401 ccagggtacg gagctcaggg tctttggaat cgaggccgga tttcagcgcg aaaacaatat
    32461 ccgtatcgtc tatggaagca aagaggccgg gcagttcttc cggcctgaat tggcccgatc
    32521 tcagtttttt gagggcggga tttgcccgga gcaggccggg gtcccggtcc aggagggagt
    32581 accagtggat cagcattttt tcggcagcca gaacggtctt atggaggtac acctgccagt
    32641 acatgatgcg ccgggattga aggaattttt caatggaatg gatgcccttt tcttccacca
    32701 ccagctggtg gtcgtggata ttgagcatgt gaatgatccg ctggtagccg atgatgcctt
    32761 ccgccacgcc ggtaaaaaaa ctatcgcggt tgagatagtc catgcggtcc aggtccagct
    32821 gaccactgac gagctgggaa aggaactttt tggggtgccg gttggtgaag attttcaggg
    32881 cctcggagag tttgccgtca aattcctggt ttaaagcccg gatcagcagg atggacatct
    32941 gctcatgatc gagggggacc agtctttttt ccagggcatg ggaaaagggg gagtggccga
    33001 tatcatgcag gaggatggct atctgtgccc cttcggcctc ttcccggctg atatccactc
    33061 ctttggaccg gaggatgttt atggcgtcca ccatgagatg gagtgcgccc agggcgtgct
    33121 ggaaccgtga atgctgggct cccggataaa caaagtcgga caaacccagt tgtttgatcc
    33181 ggcgcagccg ctgaaaccag ggatggtgga ccaggtcata aatcaaatcg ctgggcaggg
    33241 tggtgaatcc atagaccgga tcgttgataa tctttctttt gttcacagga tcaaaaatat
    33301 atcttacttt gtttacgtta ggttttactt tgacgggggc cggaagcgca tcctctgaaa
    33361 caaatcttta tggagtctgt aaaaatattg tgggcggatg acgaactgga tttactcaaa
    33421 ccgcaggtct atttccttga aaaaaaaggc taccgttttg tcctcgtaag caatgggtac
    33481 gacgcgctgg aagaatacca gcagaacaag gattttgacg tcatcttcct ggatgaatcc
    33541 atgcccggct tgtccggcct ggagaccctg gccaaactga aggccatcaa tcccaatatc
    33601 ccggtggtca tgatcactaa aaacgaggcg gaaaacatca tggaagaagc catcggctcg
    33661 cagatcgacg attatctcat caaacccgtc aatccaaacc aggtcctgct caccctcaaa
    33721 aaaatcatcg acaataaaag gctggtccag gaaaaaacca gctccgacta ccagcaggaa
    33781 ttcaggaaca tcagtctgcg gatcaatgaa ggccccaatc tcaaaggctg gatcgatatt
    33841 tataaaaaca tcgtcggctg ggaactgaaa atggaccagg cggaaaatcc cgaaatgatg
    33901 cagatcctgg ccatccagaa aacggaagcc aacagcgaat ttttcaaatt tgtgacccgg
    33961 aattacctgc aatggattga aaaagcgggg gcggaagtca ccctttccaa taccctgatc
    34021 aaagacaagg tctttccttt gctggcccag ggtaatcctg tggtgatgat cctgctggat
    34081 aacctgaggt atgaccagtg gaaaatgatc gaacccagga tctcggaaag ataccgggtc
    34141 atcgaagaag aagtgtttct cagcatcctg cccacctcca cccagtacag ccggaatgcc
    34201 atcttcgctg gtttgatgcc cgcccagatc gaacaacgtt ttgaagactg gtggctcaac
    34261 gataatgaaa aaggaggtaa aaaccttcac gaagcggaat tgctggagcg ccagctcagc
    34321 cgactgagcc ggaccccggt aaaacacagt tataccaaaa tcacccaggt cagcgatgcc
    34381 cggaacctgc cggacctgac ctcccagatc atgaaccagg ccctgacggt gatcgtctac
    34441 aattttatcg atatgctttc ccatgcccgc acggaaatgg aagtcctcaa agaactggcc
    34501 ggagatgaaa aatcctaccg ttccctgacc caaagctggt ttggcaattc tccattgtgg
    34561 gcgctcctgc aaaaactttc agagaaaaaa gtgacggtcg taatcaccac cgaccacgga
    34621 acgatccggg tgaagacgcc ggtaaaggtg gtgggggacc gtgaaaccac aaccaaccta
    34681 cgctacaagg tcgggcgcaa cctgaattat gaccggcggg aagtgtttga aatccgcgag
    34741 cccaaaaaag ccggcctgcc cagtcccaac atcagctccg cttatatttt tgcacgcaac
    34801 gacagttttt tcctttatcc caacaattat aattattacc acaactatta cctcaatacc
    34861 tttcagcatg gaggcatttc gatggaagaa atgatttgcc cggtggtggt tttatcaccc
    34921 cggtgattgc atcagggctt tgacctgcgg gtgcgcatcg gcatattgat cccacagttc
    34981 ccgttcgacc aggatgtaca gttctttgcc gaggcttcct ttttctttca gcttgatttt
    35041 gataaacttt aaaaagaaaa ggccgaaagg ccggatggat tcgatgctgt ccagggaata
    35101 ccggtaggtc cggaaataat tggtgatgta aaaatgatcg gggctggcat cgagccttag
    35161 cagtttccaa acggtaaatc gcagggtgag gatgcccgcc aggaccagtc cgggcatggc
    35221 caggcggtag atccaggtgc cgaataccgg gctgaggtcg gctccctggc tcagggtggc
    35281 cagggtgagg gcccccataa aaacaacata aaaaacggga acaaacaggc gcaacaccag
    35341 ggtccagcct gacgaaaggc ggttcattta atccttgttt ttggattcgt cgtaatcgta
    35401 ttcgtccttg tccttatcct catcctcttc tttaggcaaa ggattgaccc ggcccgcgat
    35461 ccagatgctg acctcgtaaa gcccgatgag gggcaggctg accaggattt gggaggccac
    35521 atcgggaggg gtgattacgg ctgcgatgat gatgaccacg acgatggcgt gcctgcggaa
    35581 ggtcctgagc atttccccgg tcaccaggcc taccttggcc aggaaataaa ccaccaccgg
    35641 caattcaaag accaggccga gcgggaggac aaacatggtc aggtagttga cataggaact
    35701 cagggtcacg gtatttgcga tctcgttgct cagctcgtat tggctcagga aggtgacccc
    35761 aaaaggcgta acgacaaaat aaccaaatgc gactccgaga aaaaataaaa aggaacatat
    35821 gcccaccacg cccctgatgg ttttttgttc tttttcataa agtccgggtt tgataaactt
    35881 ccagacctcc cataaaatat agggaaaaga aaccaccacg cccaaccaga aagaggacac
    35941 catatgcatc ataaactgct cgccgatatc gcgggtgatg atctctattt tatccggccc
    36001 gaaacagagt ttgggggaca ggtcgcaaaa gaagcgatag gtgaaaaaat ccttccggat
    36061 cggggccagg atgattttct gccatacaaa atccttggaa agaaagacga cgatggcgaa
    36121 aacgaggatg gaggccaatg ccctgatgat atgccaccgg agttcttcca agtggtctaa
    36181 aaaggacatt tcctggtcgc ctttgttttt catttatgga cggcaaaaat aattgcaaaa
    36241 ttcggttcag gcgataattt taagacggcc ggccttaccg ccctgatgtt atcgcatccc
    36301 aacaccttat ttccttattt ttgcgttctt aataacgtaa ccaatcccgg actcatcacg
    36361 tgaaagcctt caaaaccatc gcatacctgg ccctggtctt gttttgtttg agttcccggg
    36421 aattgccggg acaggatccc gcggtgtaca ttccctttga ttcctgcaac ctcaaagaaa
    36481 aaaacggggt gctcacgggc cagtcggtcg gcgccctggt ttgtgaatgc ggggcggcgg
    36541 gatcggcttt gaggtggaat ggaggatttc atttcgcaga ctgggaaggc aatgccctgg
    36601 acaacctgtt ctccggggat tttacggtga gtttttattt ccggcccgat gcggtcacgg
    36661 gcaccatgga tattctttcc aaacgcgaaa actgcgggat cgacagcctt tttgccatcc
    36721 ggtacctttc cgatgagagg accattgtgg cggaactggc ccagcgcgtg gaccagggcg
    36781 gtaccctcca gggtcatttg ccggaaaaca gctgttggta tcatattgtg tttacccggt
    36841 ccggcaggcg cctggccctt tttgtcaacg gggaagaaaa ggattccaag accaataatt
    36901 atatcatcga cccgcgcaac aatgccaaac tggccctggc caacagcccc tgcctcgggg
    36961 ttaccgaccg gagattcgtc ggtctcttcg atgaattgcg tttgtacaaa cgcgccctct
    37021 cccgggacga gatccgggga ctggacctga gaccggaaca tatagcaaac cgggatactg
    37081 tgattttcaa aggaggcagc gtacaggtcc gggtcaatac ggattgtagt gcccaggccc
    37141 aatggagccc ggtccggggc gtagccgatc cattaaaaag aaaccccctc atcgtcccgg
    37201 acagcaccac gacctatgtc ctgaggtccg attttggctc ctgcgtggtc cgcgatacct
    37261 tcagggtcat cgtgatcaat gaagccgacc tggattgtga ccaggtcaat ctgcccaatg
    37321 cttttacccc aaaccaggac ggtctgaacg atgtctacag gatcaacaat ccttacgtga
    37381 tcgaagacct tcatgaattt gaaatctttg accggtgggg tgaaaaagtg ttctccacca
    37441 acgacagcaa cggcgcctgg gacggcgatt tcaggggcaa gccgctcaat ccgggtatat
    37501 tcatctacca cctgaaatat ttttgccgcg gagttttaag gcataagacc ggcagcctga
    37561 cccttatcag gtagaactgt ggaattgatg aactgtaaaa ttgcctgacc attcagcaat
    37621 tcagcaattc aacatgaatt ccgccctgct cctgatttga tgatctctaa ctggagtact
    37681 tgccaaccat ccgatgaact tcatatacat acctgactac cgtatgttgt caaaccccgg
    37741 ccttcaggct ggggtttgac gctccaggct ggatatgggc tttagccccg aaccagggat
    37801 ttgtgactgt tacaacaaga gtaccataaa gtaatcccct cactggctaa ctgttgaatt
    37861 taactgttga actgtttgaa ttgtagaatt tcctgacaat tatacaattc ggcaattcag
    37921 caattcattc cgccctgctc ctgatttgat gatctctaac tggcgacctt gccaacgatc
    37981 cgatgaactt catataaaag cctgactacc ggatgttgtc aaaccccggc cttcaggctg
    38041 gggtttgacg ctccaggctg gatgtgggct ttagccccga accaggaatt gtgactgtta
    38101 caacaagagt acaataaagt atatccccta acaggctaac ggttgaattt aacttttgaa
    38161 ctgtttgaat ggtagatttg cctgacaatt ctacaattca gcaattccac aattcagcat
    38221 ctactccgcc ctctgcaccg tcatcccact gattgcaggc gcgaaaaaaa tgcaatttag
    38281 aaacagctta ttcgttccgt accagaaggc ccggaaattg ggattgtcgg ggatgcagat
    38341 cacttttcct gcaccggtac cgcttaccag cacggctgct gagtttttca actggtccag
    38401 attcgttttg gtaatgtaac cgctcaacaa cggatccgaa gtgtagcgga agggtgccgc
    38461 gtaagcattt ttcggaattt ccaggtatat gcggtttgac cggaacaggg gaagattttt
    38521 gccggaataa ccataggaaa gcgggtgggt ctcatcaaaa taagtgttga agatggcgcc
    38581 ggataccagg tccgtgccgg cgtccgcaga ggcctctgca taagcccgca tgggggtttc
    38641 tcccgattta atctcaaagg aggattgatt gggcttgact accgcggcgc tcatgccctg
    38701 ggcggccagc caaccgatgg cgcggcccat ggccacgata ttgcctccgg ccgctgccca
    38761 ggttttcagt tttcctgtcg ccgcggcacc cattccattg tattggccat ctacgaggat
    38821 cagggtattg taatcctgga aattaaaatt gttcaggccg gccatatccg ccagggcgag
    38881 gggcatggcc atgcgctgat ccaataaatg ccagacttct cccgcgtcgt tggcatccac
    38941 cccatctcca acgatcatgg ccactttagg acttgaaagt acagttgaag aagggctgcc
    39001 cagatccacc ccgctgaccg ccaggccggt gttcagagga tggatctcta ctttatacct
    39061 ggaggcaagt tctcccaact ttgccaggag cgcttctttc ccgatcggtt gatcctggac
    39121 cgggatcagc agggtgccgc gcctgaattt tttatttccg gcgggagtcg aaatttcaaa
    39181 atcctcgtgg atcaggcgta cgcggacccg ctcctgaagc aggcggtata gggctgccgg
    39241 ggcatagtaa gattcccatt ccagggcata ggcataatca ctggatcccg tgagctggga
    39301 tttgagtaca ggcagggtat tgacggcttc tccgagcagg ctgcttatcc catctgcggc
    39361 tcccagttcg agatgttcca ggttgaaagc gagcaggtag gaccaggcgc tgatgtcata
    39421 aaacaggctg tcttcaaatc ggttcctttt ctcaaacatg cctttgatca gccggtattg
    39481 gttttgccct gcagggacca cataggcttc ttccggtttg aacgatttga atgatctttt
    39541 taaggcgtgg accttgatct gattggcgag caggatgtcg atgaaatggt tcatccgcga
    39601 acgatcccgg ccggcgctga atacataacc tttcaccggg tctttggcgg catcctccag
    39661 ggcgttttta taaaaatcac gctggtaagc cagtaattta tcgtgcattt ccaccgccgc
    39721 tttataggtt gaaaaggccg ccctgacctg gttgcggatc gtaaaaggaa aggtcagtac
    39781 cccgttggcg gtggactggg cgtggccgcg ggaactggcc tgctcaaaga ggatcccgac
    39841 cgcgccgttg gcgtccggat aggtggagcc tttgccataa taataatcgt cgtaaccttc
    39901 gcgggtgtag tagagggagc cgatggaatt cagcgattcg ctgtggtagc ggccgatttc
    39961 acgggtcaat tcctggttta atttcggggt attcggattg acccgggtgg gttctccggg
    40021 cataaaaaag aaggtggcgt tggagcccat ctcgtggtgg tcggtgagga tattgggttt
    40081 ccattgatgg tagagattca ccctgcccct ggattcgggc agttgttgcg gcatccagtc
    40141 ccggttgagg tcaaaccagt aatggttggt ccttcccctg ggccagggtt cgttgaactc
    40201 ccgggtggag ggatcggtga ccatggcttc actgcggtgg gcattcaccc aggaagaaaa
    40261 ccgctgcacg ccgtccggat tgaagcaggg atcaaaaagg atgatggtct tgtccaataa
    40321 tttttcgacc tcttcgcccc gggcggcggc caggtaatag gccatggcaa gggctgcatt
    40381 ggccccgctg ggttcattgc cgtggatgct gtatccctgg tacaaaacca aaggggtttc
    40441 ctccagcctg gcccggtccg ataccgcggg atcgtaaagt tcgagatgct tctgacgcag
    40501 gctttcaagg tttgcctggt ttttctcgct ggtcacaata aggcagatca aagggcggtg
    40561 ttcaaacgac cgggcatact ccaccagttt tatccgtttg gattgggagg ccagggtttt
    40621 catataataa tagagctggt catggctggc atggcgctca cccacttcat aaccgagaaa
    40681 agccttgggc gtcgtgacgg ccggatcata ttccagttcc gggagataat aggatagggt
    40741 tatcggggaa aaggattggg attgcaacct gatcaacagg gccaggcata aaatcagtgc
    40801 ggcaattttg gatttcattc agtgagtatt tttattgggc aaaaatagaa taataaaccg
    40861 tttacatcag ctatcagctg gctgcttact gactattgac tattgactgt tgactattga
    40921 ctattgacta ttgactattg actattgact attgactatt gact
//
LOCUS       DAOQXT010000033        40778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1095_length_40778_cov_5.987673, whole genome shotgun sequence.
ACCESSION   DAOQXT010000033 DAOQXT010000000
VERSION     DAOQXT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 40778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40778
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1095_length_40778_cov_5.987673"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKM27_07730"
     CDS             <1..648
                     /locus_tag="PKM27_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNR07188.1"
                     /translation="GSEVYVSIDGRLKAVYTVSSPFKPGAPDLLQRLSGYTLSLLSGD
                     RPESLPALKKQLPARMKILFGQSPQQKLDYIRKLHESGDRVLMVGDGLNDTGALRQSD
                     IGLAVVDRHFSFSPACDILVREDRLKDLDRLIKMAVQGKYLIWAGFGYSIIYNVIGIY
                     FAVTGRMSPLVAAILMPLSSIGVMSLAYLGVAWIGKRSGLITKEPSPGDSKTNQQ"
     gene            645..1871
                     /locus_tag="PKM27_07735"
     CDS             645..1871
                     /locus_tag="PKM27_07735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNR07189.1"
                     /translation="MKERKQSIEFIKKQGLNAFLDYASVGIILVDSKMEIILVNRFVQ
                     EMFGYTKEELQFKKIDLLIPARFHTHHDKHQARYFEHPQNRPMGLGMDLYAVKKDGTE
                     FPVEVSLGTYKIGDEIFVISFVNDITLRKENEASIKRLNAELEKKVLERTEALAEAIV
                     KLQNQIKETEEAESELAVSLAKEKELGELKSRFVSMASHEFRTPLSTINSSAYLLQKY
                     KTTEDQPKREKHLERIISSVNTMTDILEDFLSVGKMEEGKVIAKIVECDIQEQVNHVI
                     NQMKPILKEGQEIQYSHVGPARVWADPSLMKHICLNLLSNAVKFSPPHQTIQVRTDTS
                     QRPWVLMVEDKGIGISKEDQKYLYDRFYRGANASNIQGTGLGLHIVSKYTSMMDGTIT
                     CESELNVGSRFILKFP"
     gene            1922..2983
                     /locus_tag="PKM27_07740"
     CDS             1922..2983
                     /locus_tag="PKM27_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR07190.1"
                     /translation="MKKILLIEDNDDIRENTAEILELAGFSVIIAKNGKEGVEKANAE
                     KPDLIICDIMMPVLDGYGVLHMMQKSPELHQIPFIFLSAKAERTDFRKGMELGADDYI
                     TKPFTGTELLSAVERRLQKYETLKEEFAPGLDGLEQIIRLNQQNRDVVKELTADRNIN
                     KYKKKQIIYTEGNRPSRLYYILKGKVKLIKTNDDAKDLVVGLYKEGDFLGYTALFEGD
                     VYKDTAEAIEDTELAVIPREDFEELLNRDIIVLRKFAKLLAKNVAEQEEHLISLAYNS
                     LRKKVAEALISLSRKYKSADQQHFSIEISRENLASIAGTATESLIRTLSDFKSEKLIE
                     IQGGKITLLNENKLEHLMN"
     gene            complement(2984..3394)
                     /locus_tag="PKM27_07745"
     CDS             complement(2984..3394)
                     /locus_tag="PKM27_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07191.1"
                     /translation="MKTMVSKRFKHEQDRFKHEYDRFKHEYEMWNTTVEILQDENVDM
                     KNKMSDILHRSNTTGQLLERAEAFQSRFLEEDEALRKFRREVLNFIKQSQNMGENGEL
                     SNGVEKMRFKLELLKKEFLQLKNDFDKFSGETGA"
     gene            complement(3481..3927)
                     /locus_tag="PKM27_07750"
     CDS             complement(3481..3927)
                     /locus_tag="PKM27_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR07192.1"
                     /translation="MTTRALSKNREFFPSVFEDFFKPWNEWFQPNDFMARTLTVPAVN
                     VVEHKDEYLVTLAAPGMAKEDFKIDLDGNLLTISSEKEETKEDKDEKFSRKEYNYSSF
                     CRSFTLPETVLRDKIDAKYEEGILKLVLPKKEDAKRASTSKMIEVK"
     gene            complement(4040..4489)
                     /locus_tag="PKM27_07755"
     CDS             complement(4040..4489)
                     /locus_tag="PKM27_07755"
                     /inference="COORDINATES: protein motif:HMM:NF021536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HNR07193.1"
                     /translation="MNFESYAAKGNELVNHLSSDLGVPRDQAARILRSVLHALRNRIP
                     LDENFDLLAQLPMALKAVYVDGWKPGKTVSQSSHLKDFLDEIRDADAQLAAYDFGNDA
                     KAKEMVLGVFRVLAQFVSSGELNDVVAGLPREIKNFITTILDENQLF"
     gene            4625..5215
                     /locus_tag="PKM27_07760"
     CDS             4625..5215
                     /locus_tag="PKM27_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07194.1"
                     /translation="MFKILLRLLLVLLVVWLGLTIWVNLKGPSANWDYGQPRSGFRAM
                     IVFDPDPIYDLDLQLCREFALVLESKGWFVKVMTVRYAEQMETSHPDLWVLCANTYNW
                     QPDRSIRGFLKNTELADQAVVAITLGAGSTANAQNGFEKLINMAGARLIHSSAYWLLR
                     PNDENKRRENNVEAAKAQVRRRAAQLADSLFMHKPL"
     gene            5244..6254
                     /locus_tag="PKM27_07765"
     CDS             5244..6254
                     /locus_tag="PKM27_07765"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNR07195.1"
                     /translation="MDSITHLALGACMGEAFAGKKLGRKAGLWGALAQSIPDIDFLAS
                     FWMDTASNLLAHRGFTHSILFCALITPVFAILAERWHRPHNISLSRWLLFFGGVIFTH
                     ILIDAFNNYGVGWFEPFSHLRMSFNAIYVADPFFALGPILAAVALIYLPRTNLLRRPV
                     WLFGLGLSGLYLLYCLANKYYIDRTVRDILANQKISYTRYFTTPAPLQNWLWYVVAGD
                     DRGYHVGFRSVFEDRPTMDFEYFPVNDSLLDETHDHVELNKLKRFSQGFYTVEKWGDT
                     LVFNDLRFGQIIGWQDPRQKFVFHYFLQHPDDNTLVVQRGRFKHWDWAVARNLLVRIF
                     RD"
     gene            complement(6263..7432)
                     /locus_tag="PKM27_07770"
     CDS             complement(6263..7432)
                     /locus_tag="PKM27_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNR07196.1"
                     /translation="MYLSPDRSYSNNNRVQFIRGGSAFFKLMEQLIGEASDTIHLQYY
                     IIEDDQTGRMIIEPLIRASRRGVKVYILADGYASQGFPDASIHRMKEEGIMFKFFEPL
                     LKSKYFYFGRRLHHKIFVVDTTRAMIGGLNISNRYNDLPGQPAWLDFALFAEGQVAVE
                     LCILCWKTWNRFPLKMGLTPCEKVPRPEIPQGSRAYVRMRRNDWVRGKNQISNTYYHM
                     FRTARKQVTLLSSYFLPGKTLRRLMSKARKRGVRIRVISAGASDVMLVKYAERWLYDW
                     LLRNEIELYEYQKNILHGKLGICDYEWFTLGSYNINDISAYASVELNLDVIQPAMTQD
                     MLKLMDKIIAEDCVRITLEKHKKSKNVFIQFGRWLSFEFIRVVFYLFTFYYQQKK"
     gene            complement(7699..8328)
                     /locus_tag="PKM27_07775"
     CDS             complement(7699..8328)
                     /locus_tag="PKM27_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNR07197.1"
                     /translation="MIFANRSEAGTILAAKLWKYREEDAVLMAIPRGGVPVAFEIAKS
                     LGLPMELALIKKIGHPNNKEYAIGAASLTDYFIVPFEKVSQDYVTAEVQRIRKRLDEM
                     RSKFMGGKEVKDIKGKTVIVVDDGIATGNTLMATLQVLRKGNPSKIVIAVPVASTNAV
                     RKLKPETDELICISVPEEFYGVGAFYKDFDQVSDEEVIEYFRMLQSMPG"
     gene            complement(8338..8988)
                     /locus_tag="PKM27_07780"
     CDS             complement(8338..8988)
                     /locus_tag="PKM27_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNR07198.1"
                     /translation="MELQFEDEVIIPSGKVELKGDLIVPVDAKAIVIFSHGSGSSRHS
                     PRNKMVARYLNKRHIATLLFDLLTVEEDLNYENRFNIPLLAERLKDATRWIVYNPVTL
                     DCRIGYFGASTGAASALIAAAGMKEIGAVVSRGGRPDLAGSSLQKVTSPTLLLIGSRD
                     PEVIELNQYAFNLLGGEKKMEIIPGATHLFEEPGTLEKVCELAYGWFDAWLIQSNP"
     gene            9108..10442
                     /locus_tag="PKM27_07785"
     CDS             9108..10442
                     /locus_tag="PKM27_07785"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HNR07199.1"
                     /translation="MGRYFSKPLKISGEDTVSVIKQWSYPLPGPSALDPLIKNIGDAG
                     IVMLGEASHGTSDFYLWRWHITRRLIEEKGFNFIAVEGDWPDCYRLNRFIKNDPKAGK
                     DAYAVLNAFNRWPTWMWANWELVAFAGWLHHFNKEKPSNRKVGFYGLDVYSLHESLQE
                     ILAYLHKTDPSAYEVALEAARCFEPYRKNEGLAYARAAELVPELCQTEVVSLLTEIRA
                     RITSYNTDPENVFSVAQNSTVVANAEQYYREMIRGGAHSWNLRDRHMHDTLVKLLDFH
                     GSEAKAVIWAHNTHIGDASATDMIQEGMYNIGELARLHLWDKGVYLVGFGSYQGTVTA
                     GHRWGGEMKIMDLPPGRRGSWEDYLHTASPEDKLLLMEDFKDDILMEHHLPHRAVGVV
                     YQPEYEQYGNYVPSILPLRYDAFIHIDESRALHPLHIKPSGGQVPETYPFGV"
     gene            10452..10871
                     /locus_tag="PKM27_07790"
     CDS             10452..10871
                     /locus_tag="PKM27_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07200.1"
                     /translation="MPRSMSNTVKIILLVLLGFVASTSVFSGLAICLNPDGSTLQLDP
                     EVLRHTVFRDFMIPGLILLIIVGGSSLAGALSIVFDHPSSQGWVLAAGIILVGWIAGQ
                     VVLIRTYHFLQLVYLITGILVAGLSFFIKPTTEIYRV"
     gene            complement(10901..11947)
                     /locus_tag="PKM27_07795"
     CDS             complement(10901..11947)
                     /locus_tag="PKM27_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNR07201.1"
                     /translation="MESKEIKLQQFFYYTALLLSVLFLLYAGRTLFIPMFYGLLIALV
                     LYPFCRGLEARGWNKTVAIVLGMIIVTLLILFLGFLLILQIKALQQDFPELKARFLAN
                     IPALQAWIQTTTGISQAVQDQWWSDFKSSPSVFSGSAIRNTMSATLNTIYIAYITPVF
                     TALFLYNRKVFVQFLSSLAGEEYKDKLSVILRETVTTYANFIKGMIKVYLIVGTLNSL
                     GLLLLGIKHALLFGYLTAIMTIIPYIGIFVSSMLPISMAWLTKGNILYPLGVIAVFSF
                     VQYLEGNVIFPRVVGSQLNISTWATLVAIIAGGILWGISGMILFIPFIGMLKIVSDYI
                     PEWKALNILISRRP"
     gene            12068..13729
                     /locus_tag="PKM27_07800"
     CDS             12068..13729
                     /locus_tag="PKM27_07800"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNR07202.1"
                     /translation="MGLILFKLLAGIVIFLLGFSFIEEAIRNVTGRAFKLFLKKHTSQ
                     PVRGLVAGALVTAILQSSSIVNFLVLSFVGTGVLNLYQALAVMLGANIGTTVTSWIIV
                     LVGFKLNLEDLAFPLIALCGFFTFLYKKQNILFFWMRLLFGMGFLFFGLEYIKISVFE
                     VIASFDLAFLNQSPLLLFVLFGLIATTLTQSSSATVVLALSALNAEAISLIAAMAIVL
                     GSEVGTTLKLVLAALDGPVIKKKVALGNLVFNSVTLVVAFTLIRPTHSLIRDFIGIGD
                     HLVALVFYQSLINVGCAILFLPILKKLGHWLEVLSFRDSDDTSIIRKVKVTDGDYAHE
                     ALETETHQMMQWVLVFTRKAFRLENPAEPLPGISSKMAGMDRLQWYNHLKSLHGEIRN
                     YAFNLQRLTTEEEKLNRIDQLLETVRDAMFAVKSIKDSFHDIDLLSNSSNDVKFGFYM
                     ETRQKVMELVDEIGGILQHPEAQPVYDQLFSNVKKLEDHYHTMVGRFYKDGFDIALSG
                     DEFSTLINFNREVFNAGKYLIIMLKEYYLSAQQAELFDEQLHAGL"
     gene            13842..14939
                     /locus_tag="PKM27_07805"
     CDS             13842..14939
                     /locus_tag="PKM27_07805"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07203.1"
                     /translation="MNVKNYPEITEIVEHKNYLPSISVIFPFNIKVGMGVELKHKLKT
                     KRDEIEKELLKSYPADVVASLMSKYTSLTENLKPQNMHQGLAIFLSPWFQKTYFLDFE
                     PQEKNIIDSSFEIRDLIYNKNAVSKAILLLLSANEIRFYLVSPAHVTKLNVDISNRIE
                     AFMANTLHDVEIHHEKSERRDKAMQLFLQNADEGIALALKDHACPVIVAGSEKYIGHF
                     KKMTKHGEVIEDYIEGNYLESTPAELNQLARESFGRIQETRSRKIIDQVEDALNKKLL
                     ATGLKEVWKQATEKNGRILVVEKNYRKPAAQGAVDEEIITIGLDHVPFPMEDAVDDVI
                     EKVILNGGMVHFVDDGLLGKYRRIALITYHQ"
     gene            complement(15140..15230)
                     /locus_tag="PKM27_07810"
     tRNA            complement(15140..15230)
                     /locus_tag="PKM27_07810"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15194..15196),aa:Ser,seq:gct)
     gene            15303..16646
                     /locus_tag="PKM27_07815"
     CDS             15303..16646
                     /locus_tag="PKM27_07815"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HNR07204.1"
                     /translation="MKIPVWRWILKAFSCGIRAIIFLIPGFSSGQVRIDTLYPSAWSA
                     NSVNAVIFRKHSLTSNTQYQFTSFYDTTGTVVVGRRSPGSGSWEWKRLNYKGRAGDAH
                     NCISIQLDGQGYLHLAWDHHNNRLNYIRSSAPGSLDFEPMQAMTGHLENQVSYPEFYR
                     LPDGNLIFLYRDGSSGNGNLVINRYDCASRTWSRLHQVLIDGEGERNAYWQAAVDNKG
                     GLHLSWTWRETPDVASNHDLCYAVSRDGGLHWENSLGQAYRLPITAATAEYIRRIPQG
                     SELINQTSMTCDGSGRPFVASYWQDPGEEAPQYHLVFKRGKKWISSSGGFRKTGFSLK
                     GTGTRSIPISRPQVLVWGKGKTFRAGILFRDQERDNKPSLASSKGPGFRNWMVSDLDE
                     ESLDRWEPTFDPDLWGSAGILNLFLLKVRQLDAEGLGMDQGSPVRLLEWQPPQNQ"
     gene            16830..19220
                     /locus_tag="PKM27_07820"
     CDS             16830..19220
                     /locus_tag="PKM27_07820"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNR07205.1"
                     /translation="MKPSRIKSRLWPVLFLAGYAWLSSAQSTSNHGNKFEQLGTILPT
                     PNEYRSASGAPGPKYWQQRVDYDIKCELDEENQKLTGSETITYFNNSPDVLNYLWLQL
                     DENQHSSVNNANYQNTSNMPQVLTDRVIDRVEEYKTDNGYGHIIHKIVDATGKPLVYT
                     INKTMMKVNMPVPLKPNSVFVFKIDWSYKLVDRMDFNRFQNARGGYEYFQEDGNHIFT
                     IAQWYPRLCKYGDEIGWQNHQFTGRGEFTVSFGNFKVQMTVPADHVVGSTGECLNYTT
                     NLTPTQKLRWQKAQTAKEPVQIVTLDEALAASKDKSKAKKTWIFKADNVRDFAWTSSR
                     RFVWDALPIQVNNKKVMCMSFYAKEAYPIYSKYSTKAVAHTIKTYSDFTIPYPYPVAQ
                     SVEASNGMEYPMICFNPGRAEKDGTYSEGNKNAAILVIIHEVGHNFFPMIVNSDERQW
                     TWMDEGLNSYMQYITQELWDNKFPSDGGPPHTIVDYMKWPKDRLEPIMTNSENIIGFG
                     PNAYSKVATGLNILRETIMGRELFDQAFKEYARRWAFKHPTPADFFRTMEDASGEDLD
                     WFWRGWFYSIDAADISIDTVKYATADVHAVPSRLTDTMVMRPLAKPDVNDFEDLSKIR
                     NRQDTNIVFVTDRDTSLRDFYWKYARDIAKYDTTKYPVTLVANQDTLDAEAKKKYGNK
                     HMYEITFSNKGGLVMPIILEWKYKDGSSEIQRIPAQVWRYNENKITKAFLKDKKIANI
                     KLDPYRETADIDESNNVYGEEKPIPEPSRFKVFKQKQVTPAPGVNPMQKAAEKAKT"
     gene            19309..19746
                     /locus_tag="PKM27_07825"
     CDS             19309..19746
                     /locus_tag="PKM27_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR07206.1"
                     /translation="MKFNVFIFILAASWLQRLPAQTDLETIRQIQEVCTYYLDGGTLG
                     DSLLFSKAFVPDGQMRYIRNDTLMNVSLGDFMARARNNGVRQNRKTKIESIQVYGNAA
                     TAKLTIEYPSFYFHDLMSLLKTSEGWKIVGKIFYREEKSPPVN"
     gene            complement(19743..22097)
                     /locus_tag="PKM27_07830"
     CDS             complement(19743..22097)
                     /locus_tag="PKM27_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNR07207.1"
                     /translation="MVFRSLVMLCIFLVGYFPPVPGQSLVPELGNRNIRVNPIVPIKS
                     YAFNLKEVSLLPGSPFYHARRMDSIYLSLLSPDRLLHRFHKNAGLAPKDSIYGGWESG
                     GLSGHTLGHYLSAAAMMYASTGDRALKKKIDYTIKELGRIQTTRKTGYLGAIPNEDSV
                     FLKVSKGMIRATGFDLNGAWSPWYTVHKIMAGLGDAYIHAGNREALEIVSRMADWVHQ
                     TIAHLPDSARLRMLDCEYGGMNEVLANLYAFTGNKKYLDLSYLFYDEFVLGNLAQGID
                     PMPGKHANTNVPKVIGSVRQYELTANPRDYRIADVFWNAMVNHHSYVIGGNSNYEYCG
                     EPDRLYDRLSDNTCETCNTYNMLKLTRHFFALSPDSRYMDYYERALYNHILASQNPEN
                     GMMCYFVPLRTGAKKTFSDTFNTFTCCVGSGMENHSKYNEQIYSHDGSKVVYVNLFIP
                     SSVHWKSRKLLLEQQTLFPARDEVDFWLRSNPRPGTTLAIRHPFWSKKINITINQQPA
                     LKQYMRNGYLFLEHPWKKGDRIRISLEKTLYTEPMPDQADRIAIKYGPLVLAGLLGDS
                     SPPEPEIPVLVSKERDPNHWIAPGNQALTFSLTGTRPSPVMLKPFYQVYDQHYTVYFD
                     FYTEAQYVQRLRDYQAALDLKKALEERTIDFIQPGNEEDEERHGLSHSERSYVDAALG
                     RTGREARTGHYFEFRMKTHPGLPTQLVFTYLGDDQNRSWDILVNGQKLTTVEWPGGHS
                     GKFYDVFYDLPPALNMGSEILVRIEANQGKTAGRVFGVRTVRAT"
     gene            complement(22222..23604)
                     /gene="dnaA"
                     /locus_tag="PKM27_07835"
     CDS             complement(22222..23604)
                     /gene="dnaA"
                     /locus_tag="PKM27_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNR07208.1"
                     /translation="MHQIVWNNCLKAIRDAIPEQSFKTWFEPVKPIKLEDDVLTIQVP
                     NKFFYEWIEEHYLGILKKCIRHEIGKQGRLEYFIPMENQTRSSAGEPSFPKPLDSSHI
                     VNPFVIPGIRKAKVESNLHPDYRMSNYVEGDCNKLARAAGIAIARKPGGTAFNPLFIY
                     GAVGLGKTHLVQAIGNEILANHPDKNVLYISSEKFTQQVIQSIKNNAIDDFMAFFHNL
                     DVLIIDDIQFLANRTKTQEIFFHIFNNMHQVGKQIIISSDRPPKDLQDVEERLVSRFK
                     WGLSADIQAPDFETRMAILDAKSQLEEIQLPYPVLELICHNIKESVRELEGVLISLAA
                     QASLNQRQIDVLLAKEVIEKFVRNYTREITLDQIQKMVAEHFNLKEEYLQGRTRKRSV
                     VIARQLSMYLAKNLTTYSLAKIGGTFGGRDHSTVIYSVKTIKDLLDTDDGFRSTVSEL
                     EKKIKMSMAV"
     gene            complement(23806..24729)
                     /locus_tag="PKM27_07840"
     CDS             complement(23806..24729)
                     /locus_tag="PKM27_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HNR07209.1"
                     /translation="MKRRDFLLQSGLGGGWLLGQGIPWRSWAPEEKVKLTILHTNDVH
                     SRIDPFPMDGSRLQGMGGAAARAAMIKKIRAEEKNVLLLDSGDIFQGTPYFNLYKGEI
                     EMKTMTAMGYDAATMGNHDFDGGLEMLKTQLPHARFPLVVSNYDFRDTPMEGQVKDYF
                     VKELDGLRVGIFGLGIEMKGLIPDALVGNTRYLDPVNKANQISLLLKEEIKCDYVICL
                     SHLGYQYRNEKISDLRLAPLLNHVDLILGGHTHTFLDKAQVVKDLGGNPLIINQVGWG
                     GVLLGRIDIEFERNKTRHRCKTCSNEWIMNG"
     gene            complement(24735..25469)
                     /locus_tag="PKM27_07845"
     CDS             complement(24735..25469)
                     /locus_tag="PKM27_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNR07210.1"
                     /translation="MSKFKYILLAGAGMLLAQCKTYDHLAMVRADTLRVSSAAPDSQV
                     SRLILPYKSELDAKMNEVIATAPESMTKERPESTLTNWVVDALYNQASKLYTETPAFA
                     FQNYGGIRINNLGKGEVSLSQMFEIMPFDNLVVLLQLKGTEVQRFLDYVAEAGGAPVS
                     SQVRFRIENKKATDIRIQGKGIDPEGLYHVVFPDYIANGGDNLEFLKDKPRIEKNILI
                     RDLLTAEAREKGILRANLDQRITIKK"
     gene            complement(25456..25974)
                     /locus_tag="PKM27_07850"
     CDS             complement(25456..25974)
                     /locus_tag="PKM27_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07211.1"
                     /translation="MFSGKFSSILVPAGLFFIGAVLLFIYLFLKRNLGIYIRESLDRV
                     PGSQSGLILNAYERLALLCERIQPGPLAMRLQTPQLNAQALSQAMIVAIQQEFDHNIS
                     QQIYISEKLWNIILLARDEAIYEIIHTAGELSPETGSGTLTQKLMEQRQNSSLNKALQ
                     AIRNEAKLHVKV"
     gene            26147..26497
                     /locus_tag="PKM27_07855"
     CDS             26147..26497
                     /locus_tag="PKM27_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNR07212.1"
                     /translation="MNDLETETLPLTLSDSAVGQLKRIREEQQIPEDLGLRVGVKGGG
                     CSGFSYVLGFDQGQEDDMRFSTQGLEILMNKAHALYLIGIQIDWVDGLNNRGFTFTNP
                     NAKSTCGCGTSFSA"
     gene            complement(26501..26998)
                     /gene="purE"
                     /locus_tag="PKM27_07860"
     CDS             complement(26501..26998)
                     /gene="purE"
                     /locus_tag="PKM27_07860"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNR07213.1"
                     /translation="MNKIQVSIVMGSDSDYAVMKEAAHILEYFGIGFEVDIVSAHRTP
                     KYLFDFATSAHTRGIRVIIAGAGGAAHLPGMVASLSPLPVIGVPIKSSNSIDGWDSIL
                     SILQMPNGVPVATVALNAAKNAGILAAQILAAGNIRLRKKIEIYKLELSQEVVKKRKK
                     LKTGK"
     gene            complement(27036..28184)
                     /locus_tag="PKM27_07865"
     CDS             complement(27036..28184)
                     /locus_tag="PKM27_07865"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNR07214.1"
                     /translation="MDSAFDSSPVGILGGGQLGKMLAQAGADWNLNLHVLDPAEDCPA
                     ACCAKQTLGDFRDYETVLKFGADKKIITIEIEDVNTKALEVLSGKGIRIYPGPEALGI
                     IKDKGSQKQFLQSQGIPTSEFSLTSSRQEIENLVRENKLQTPFVQKLRTSGYDGRGVL
                     MVNSPADLNLLLEGPSLIEEKIRVQTEIAVLAARNTHGEVATYEPVEMIFHPEANLLL
                     YQESPASISPAAASRAREIAADLIHSMKIIGLLAVEFLIDENDQVLVNEVAPRPHNSG
                     HHTIEGNLTSQYHQHLRCILGLPLGLTQVTGHTVLINLLGEPGFSGPAKYQGIDSCLA
                     LPGVYVHLYGKKETRPWRKLGHVNILGKTREDVKEKADWVRNHLKVIS"
     gene            28230..29567
                     /locus_tag="PKM27_07870"
     CDS             28230..29567
                     /locus_tag="PKM27_07870"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNR07215.1"
                     /translation="MAKLTLIHNARLVNEGKIQEQDVLISAGRIEHIGPERKHLAAEE
                     IDAGGMVLMPGIIDDQVHFREPGLTHKADIASESRAGLAGGVTSFMEMPNTVPHATTR
                     EVLEKKYGIASEQAFSNYSFYLGATDQNLEEIKQVDPKRVCGIKIFMGSSTGDLLVAD
                     DRALEGIFRESPTLIATHCEDETMFRQKQEEYRVKFCDEIPASAHEFIRSAEGCLASS
                     SKAVNLAQQYGSRLHILHISTLDELALFNNDLPLEQKKITAEVCVHHLFFDSAQYASL
                     HNKIKCNPSIKHARHREALRKALRDGRLDVVATDHAPHTLSEKTGSYLQSASGLPLVQ
                     HSLQIMLTLHHEGEIPLEEIVTKMCHHPAICFKVKDRGFIREGYHADLVLVNPDERVL
                     VTRENILYKCGWSPLENYQLRGEILATWVNGNLAYNRGVFNPVKTVQRLEFDR"
     gene            complement(29626..30951)
                     /gene="tilS"
                     /locus_tag="PKM27_07875"
     CDS             complement(29626..30951)
                     /gene="tilS"
                     /locus_tag="PKM27_07875"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNR07216.1"
                     /translation="MPFPIERKIKSILETHPLIARGDRILVAVSGGMDSMCLISLLQA
                     LDLDLAAGHVNFKLRGGESDLDEALVRAHCSTLGLPVHSVSFDTRAEAQKSGKGIQET
                     ARDLRYDWLSEIADQYGYHKIATAHHQDDQAETLIFNLVRGSGLRGAGGIPIMQGRII
                     RPLLAVSKKEIREYISLRGIPYREDLSNLKTDYTRNKIRHQIIPLLEQINPRASAHLA
                     AFSERVQQYLPVYTLWKNQMSRLHLTRSGRKVVLSIPEPADPALLFVLLEDYGFNSSQ
                     CSNIAMALREGHKGNRFYSERFELMTIGQMAELYEVSQPPTTVDIEIPGLPFETRLDD
                     RHIEIFTCPAGEGPKSFAQADLSSVRFPLRLRTWKAGDRFAPSGMEGKTKKLQDFLTD
                     MKVSGLEKKQALVLCDADRIIWVWPGNRISEWVKCKEQSTVCLGIRCGP"
     gene            30977..31546
                     /locus_tag="PKM27_07880"
     CDS             30977..31546
                     /locus_tag="PKM27_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07217.1"
                     /translation="MSFSKFGIFILFAGLAAAACKNNADPTGDVFSQKDTAGINEMPV
                     SANAVRSDTPMVSSFTKPDAMARNKYSRFDTILIKDLCTQMWKFDGGIEGPKNITAEE
                     MQGFWMQFFPNGKYEKGMYHKITSKGNFTIDNLGFLEMTPDDSKEKKSEWQSKFNSDM
                     LILIGTEKYRDNHIQMRLSRIGKKPVKTN"
     gene            complement(31559..34171)
                     /gene="topA"
                     /locus_tag="PKM27_07885"
     CDS             complement(31559..34171)
                     /gene="topA"
                     /locus_tag="PKM27_07885"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNR07218.1"
                     /translation="MGKKLLIVESPAKAKTIEKFLGEDYQVKSSFGHVRDLEKGKKGI
                     DIENNFQPNYIVSPEKQKVVRELKDTARKAEEVWLATDEDREGEAIAWHLCEVLGLDP
                     KITKRIVFHEITKPAIQEAVLRPRLLDLDLVNAQQARRVLDRLVGFELSELLWRKIKN
                     NLSAGRVQSVAVRLIVEREREIRNFVAVPFFKVSALFRIQDAEGRVYTLKAERPERFD
                     DKKDARSFLDACITGRFSIQSIDIKPLRRKPAAPFTTSTLQQEASRKLGFSVSRTMVT
                     AQRLYEQGYITYMRTDSTALSNVAVQQITEEIERAYGKHYVETRIYKSKAANAQEAHE
                     AIRPSYINRLEVSPIRDEQRLYELIWKRTIASQMADAELEKTIVKIGISTLPGEYLQA
                     EGEILKFDGFLKVYLESSDEEEDGHDESILPPLHIGQVLDLRKMEAVQRFTRPPSRYT
                     EASLVKKLEELGIGRPSTYAPTISKIMETGRGYVVKEQREGIERKFEILTLENGQIRE
                     SMDSEITGASKNNLFPTDMGMLVVDFLTKNFEDIMTYGFTAEIEKQFDIIAEGNMEWH
                     QMIQAFYRPFHQKLEDTLQNTGRESGKRILGSDPKTGRAVFVRMSRYGPVVQIGDTEE
                     LKEDEKPEFANLKPGQSMENISYEDAMELFKLPRSLGEYQGKEVIIGQGRFGPYVKYD
                     EGFISIPRPEDPLAVSLDRAIELIEAKRAELAPLAYYKEKPITKGKGRFGPFVKWNEL
                     FVNVPAKYNFDNLDPKTAIDLVKAKEEKEASKYLSRWEEENLSVQNGRWGPVIYYGKK
                     HYNFPKNPDGKRITPEEAAAYSLEQVKSLILASDPQAFEKKGKRKTTGKAKSTRTPSK
                     KPAAKKPAKARAIS"
     gene            34366..34656
                     /locus_tag="PKM27_07890"
     CDS             34366..34656
                     /locus_tag="PKM27_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07219.1"
                     /translation="MKKHRDKIIITLLVQAVFYTLVWMVHDYTALLLSAGIGIIALAI
                     LIISWLSDRMEYGNMPSWYYPVLWISILVPLLISIVFWLLKGGQMDWMEPIF"
     gene            complement(34693..35058)
                     /locus_tag="PKM27_07895"
     CDS             complement(34693..35058)
                     /locus_tag="PKM27_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07220.1"
                     /translation="MRILLLGVWFGVILGCKSEPPLFDKNKVKGTWIVTEASRNNRIT
                     QTVNGAVFKISDTELTHNLYGSDSSYAIQWSREAIVTGQAAYMVETAQDSLLVLATIL
                     HNFPFRLSLKKTTPPQTEE"
     gene            complement(35124..36362)
                     /locus_tag="PKM27_07900"
     CDS             complement(35124..36362)
                     /locus_tag="PKM27_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNR07221.1"
                     /translation="MMSLRILIFLCILAGIDLYSFQWLRTLSQGWSPVWRGSAYTIFW
                     FGTLVTVTLFVFYFTGQTDKIPHVVLIYLRAIAFISYFSKFISIPIVLMDDIRRGAGL
                     LVQWFKPDYQFNASRSRFLSTFSAVLAGLPFVTLMYGMARNAYRYRLHRHKIPVSGLP
                     PELEGLKIVQISDIHSGSFTAKDPIKKSLDMIRELDPDLLFFTGDMVNYRADEVEPFI
                     DIFAGMKGRYGSFSILGNHDYGDYVQWTDAQAKQDNMESLKSNHEKIGWKLLLNEHQV
                     IEIKGHPISIIGVENFSASRRFSKYGNLAKAYEGVPGHSLQFLLSHDPSHWDHEVNSR
                     YPDIHLTCSGHTHGFQFGVEIPGYFRWSPSQYVYHQWAGLYQKGKQFLYVNRGFGFLG
                     YPGRVGILPEITLLELTAAI"
     gene            36643..40332
                     /locus_tag="PKM27_07905"
     CDS             36643..40332
                     /locus_tag="PKM27_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07222.1"
                     /translation="MKTVHQAVLYCLFIAIAHSAQAQCIEGRAFFTLSGDTTVYACPD
                     GKPDLVGFRPFTGGTPFIYVITDQNDTIIGTTLTGVVDFENLPGSEYHVYGYSFKGAL
                     GWVLGKHILDARITDYCYERSTNYVLVIKEYPVAPVINQPGTGPLFVCAPDLKADSVK
                     TEAVFGSRSKIKYLVADEKGIIVKTSDRPELDADQLECDSCKIYVLAYTGRFLGLEGN
                     SIDAALSTGCYELSSDFQLLVRDLPRGGNILYASQDSQGFICAASTKAQPLSLDLSNN
                     SAGYEHYIFTDTLNQVIALSDSASIPGDALIAGICRVWGLTFTGQLNDGYSGKDIHDF
                     IWTDDCYAVTENFLQITKALPDGGQIAFKSDAGLQILCKDGVPDSLSLEVTGALGAHG
                     AWVIVDTAGRVYDWTSSSSYDFNKLPAGEWKIYFLSYTGNLILAIGDSLHGAPSDDCY
                     DYSDSSLTVVTDVPLPGLLTFSDSTTTKFFCASSSGNLVAFLNFTSRSSLNSALLLTD
                     ANDKLVRIVDSLDLNLDSLANGNYRIYSLSYSGDLLAETGQVLDPMNLSSGCSQLSDQ
                     YLGLMIEEVEAADISLENGQDSLTWCAGDTLSTTVLGTNAMPGTPHVFFVTDLRDSVL
                     HLLDSDTLRAANLNSGASRIRGLSYSGQLLLQPGQLLSQQTLASGCAALSTNVIKVLK
                     ENPQGGRVSLVSGDTSYTICFKDGWPDRPALKSTSTSLIEYQYILTNEQNQIIDLNAS
                     ELDLESLNSGRYRIYGVSYLGSVLAKAGDDMTAVPFASGCFQYSENAIQISSDELSAG
                     TLRTPTGENVLYYCTDDSQPDTVLLTSNGATPGAKYAYLGIQSDTVKYISMDGRIIDV
                     HLPAGTTRVFGVAYRGSLLVKAGDRVSSARLTDSCFSVTSNEVIIYKYAPEAGTLTTS
                     GGQTEFFLCPQDGQPDFITVEEQGAVPLPYAYVITDEADSILAWSSNSSFDLDTFSLG
                     LCRIYGISYSGNLQLFGKKLDGPDLSSGCFDVSGQFITVYKSTADAGRISLAGGDTVV
                     TICVEDLAPDTLFFSHTGVQGLKYAYLVTDLNNRLQAIVEKDGPEFDFNGADPGTSRI
                     YGVAYGGILSVFRNDDVTATALSTGCYDLSDNFILLNKVNSGPGCKTSGIQSGNQVFL
                     SIFPNPALQRVTLSLKGDYLKSGKPELHLISVTGQLGKKINLSSEDIDNEKVVFDVTS
                     LTPGLYFIIFKNGYIFDRVKMTVIR"
     gene            40487..>40778
                     /locus_tag="PKM27_07910"
     CDS             40487..>40778
                     /locus_tag="PKM27_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07223.1"
                     /translation="MKKSAPIIFPGKFSLGLLALTFLLQGYRAWAQCLPHTISVSTLE
                     QVLYACPGDGKPDEFVFLKSDTSQNKYAFILADGADNIIRVQTSPVFNFDGAL"
BASE COUNT         9969 a        10085 c        10292 g        10432 t
ORIGIN      
        1 ggaagtgaag tctatgtgag catcgatggc cggttaaagg ccgtgtacac ggtgtcgagc
       61 ccttttaagc cgggggcgcc cgaccttttg caacggttga gcggatatac cctgagcctg
      121 ctgtccggcg accggccgga gagccttccg gctttgaaaa aacaattgcc tgcccggatg
      181 aagatattgt ttggccagtc tccccagcaa aaactcgatt atatccgcaa gctgcatgag
      241 tccggggacc gggtattgat ggtcggggat gggctcaatg ataccggtgc gctccgccaa
      301 agtgatatcg gcctggcggt ggtggaccgt catttttctt tttctcccgc ctgcgacatc
      361 ctggtccggg aagaccggtt aaaggatctg gaccggctga tcaagatggc cgtccaggga
      421 aaatacctga tctgggcagg attcggctat tccatcattt acaatgtgat cgggatttat
      481 tttgcggtga ccggtcggat gagtccactg gtggctgcca tattaatgcc tttgtcctcc
      541 atcggggtga tgtccctggc ttacctggga gtggcctgga tcgggaaacg cagcggattg
      601 atcaccaaag agccttcgcc cggggattca aaaaccaatc agcaatgaag gaaagaaaac
      661 aatccataga attcattaaa aagcaagggc tgaatgcctt cctggattac gcttccgtag
      721 gcatcatcct ggtcgacagc aagatggaga tcatcctggt caaccggttt gtccaggaga
      781 tgtttgggta tacaaaagaa gaacttcaat ttaaaaaaat tgacttgctg atcccggccc
      841 gcttccatac ccatcacgac aaacaccagg cccgttattt tgagcatcct caaaaccggc
      901 cgatgggcct gggcatggat ttatacgcgg tcaaaaagga tggaacggaa tttccggtgg
      961 aggtttccct gggcacctat aagatcgggg atgagatatt tgtgatatcc tttgtcaacg
     1021 acatcaccct ccggaaggaa aatgaagcct ccatcaaaag gctgaatgcc gaactcgaaa
     1081 agaaagtgct cgagcggacc gaggccctgg ccgaagcgat cgtaaaactg caaaaccaga
     1141 tcaaggaaac cgaagaagcc gaatccgaat tggcagtttc cctggcgaag gagaaagaac
     1201 tgggcgaact gaaatcccgc tttgtatcca tggcttcgca cgaattcagg acgccgctga
     1261 gcacaatcaa ttcttcggcc tacctgcttc aaaaatataa aaccacggag gaccaaccca
     1321 aaagggagaa acacctcgaa agaattattt cctcggtcaa caccatgacc gacattctgg
     1381 aggattttct gagcgtgggg aaaatggagg aagggaaggt cattgccaag atcgtggaat
     1441 gcgatatcca ggaacaggtg aatcatgtca tcaatcaaat gaaacccatc ttaaaggaag
     1501 gacaggagat ccagtattcc catgtgggcc cggcccgggt gtgggccgat ccttcgctga
     1561 tgaaacacat ttgcctgaat ttgctgtcca atgccgtaaa gttttctccc ccccaccaga
     1621 ccatccaggt ccgtacggat acgagccagc gcccctgggt cctgatggtg gaagacaaag
     1681 gcatcggcat ttcgaaggaa gatcagaaat acctctacga ccgcttttac aggggtgcca
     1741 atgcctccaa tatccagggt accggactgg gcctgcacat cgtatccaaa tataccagca
     1801 tgatggacgg gaccattacc tgcgaaagcg agttaaatgt gggctcccgc ttcatcctca
     1861 agtttccctg aaattttgac aactaaagtt ttggaggtat attagcccat aatggcattc
     1921 tatgaagaaa attctcctta tcgaggacaa cgatgatatc cgtgaaaaca ccgctgaaat
     1981 tctcgaactg gcgggtttct cggtcatcat agcaaaaaac gggaaggagg gggtcgaaaa
     2041 agccaacgcc gaaaaaccgg acctgatcat ttgtgatatc atgatgcccg ttttggacgg
     2101 ctatggcgtc ctccacatga tgcagaaatc gccagaactc caccagattc cctttatttt
     2161 tctttccgcc aaggccgagc ggacggattt caggaaaggc atggaactgg gcgccgacga
     2221 ttacatcacc aagcctttca cggggaccga attgctgagc gcagtggaac ggaggctcca
     2281 gaaatacgaa accctcaagg aagaatttgc cccgggcctc gacgggctgg aacaaatcat
     2341 ccggctcaac cagcaaaacc gggacgtagt caaagaactg acggccgacc gaaacatcaa
     2401 taaatacaaa aagaaacaga tcatttacac cgaaggcaac cgcccttccc gcctgtatta
     2461 tatccttaaa ggcaaggtca aactcatcaa aaccaacgat gatgccaaag acctcgtggt
     2521 gggcctgtac aaagaaggtg atttcctggg ttataccgcc ctgtttgaag gagatgtata
     2581 caaggatacc gcggaagcga ttgaagacac cgaactggct gtcattcccc gggaggactt
     2641 tgaagaatta ctcaataggg atattattgt tttgaggaaa ttcgccaaac ttttggctaa
     2701 aaatgttgcc gaacaggaag aacacctgat ctccctcgcc tataacagcc tgaggaaaaa
     2761 ggtggccgag gcgctgatct ccctttcccg caaatataaa agcgcggatc agcaacattt
     2821 cagtatagaa atctccaggg aaaacctggc cagcatcgcc ggtaccgcta cggaatcgct
     2881 gatccggacc ttaagcgatt tcaaaagtga aaaactcatt gaaattcagg gaggcaaaat
     2941 caccttgctg aatgagaata aactcgagca cctgatgaat tgatcaggcc cccgtttctc
     3001 ccgaaaattt atcgaagtcg ttttttaatt gtaaaaattc ctttttgagc aattccagtt
     3061 tgaaccgcat tttctccacc ccattgctca gttcgccatt ttcccccatg ttttgggact
     3121 gttttataaa gttcaatacc tcccgtcgga acttcctcaa tgcttcgtcc tcttccagga
     3181 agcgggattg aaaggcttca gccctttcca gcaactgacc ggtggtattg ctgcgatgca
     3241 ggatatccga catcttgttt ttcatatcca cattctcatc ttgcaatatt tctacggtcg
     3301 tattccacat ttcatattcg tgtttaaaac gatcatactc gtgtttaaag cgatcctgtt
     3361 cgtgtttaaa acgctttgat accattgtct tcatgtttcc ccgattaccc gaaaaattat
     3421 tccttagttt gctttagtgt aaaaaaagcc ctgcatcgct gcagggctta caaaacctaa
     3481 ctacttaact tcaatcatct tagaagtgga agctcttttc gcatcttcct ttttgggaag
     3541 aaccagtttc agaataccct cctcgtattt ggcatcgatt ttatcccgca gcacggtttc
     3601 cggcagggta aaacttctgc agaaggagga atagttgtac tctttgcgtg aaaatttttc
     3661 gtctttgtct tctttggttt cttccttctc actggaaatg gtgagcaggt tgccatcgag
     3721 atcgatttta aaatcctctt tggccattcc aggagcagcc agggtaacca ggtattcatc
     3781 tttatgttcc accacattga ccgcgggaac ggtcagcgta cgggccataa aatcattggg
     3841 ctgaaaccat tcattccaag gcttgaaaaa atcttcaaaa acagaaggga aaaattcccg
     3901 gtttttactt aatgctcttg tggtcatata acctcctttt tttattgttg tttactgata
     3961 gtacaaagct aaccgacggc ctttgccggc agcatgatcc gcatcatcat atattatgaa
     4021 tattatcatt cgttgattat taaaacaatt ggttttcatc cagaatggtg gtaatgaagt
     4081 ttttgatttc ccttggaagg ccggccacca catcgttcaa ttcgccggag ctgacaaact
     4141 gggcgagaac ccggaacacg cccaggacca tctcttttgc tttggcatca ttaccgaaat
     4201 cataagcggc cagctgtgca tcggcatccc ggatctcgtc cagaaagtcc ttcagatgcg
     4261 aggattgact tacggtttta cccggtttcc atccatcgac atagacggcc tttaaggcca
     4321 tgggcaattg tgccaataaa tcaaaatttt catccagcgg aatccggttg cgcaaagcgt
     4381 gcagcaccga tctcaaaatc cgggcggcct ggtccctggg taccccaagg tcggaggaaa
     4441 gatgatttac caactcattc cccttggccg cataggattc aaaattcatt gacattttgt
     4501 tctttgccgg caagatatgt ttgggcctct gcccaaaaaa tgacctttta cagccggtga
     4561 cctgaccttt gtcagtaagg agcccatgct ctgacctatt tttgaaaagg aacagctgta
     4621 ggatatgttt aagattttgt taaggcttct cctggtgctt cttgtggtct ggcttggctt
     4681 gaccatttgg gtcaatctga aaggtccgtc ggcgaactgg gactatggac agccccgttc
     4741 agggttccgg gccatgatcg tctttgatcc tgatcccatt tatgatttgg accttcaatt
     4801 atgccgggaa ttcgccctgg tcctggaatc aaaaggctgg tttgtaaagg tcatgacggt
     4861 cagatacgct gaacaaatgg aaaccagtca cccggatctc tgggttttgt gcgccaatac
     4921 ttacaactgg caacccgacc ggagcattcg cggctttctc aaaaatacag agctggccga
     4981 tcaggctgtg gtggccatca ccctcggtgc ggggtccact gcaaatgctc aaaacggatt
     5041 tgaaaaactg ataaacatgg ccggagccag attgattcat tcatccgcct actggctgct
     5101 ccgtcccaat gatgaaaaca agagacgcga aaacaacgta gaggccgcca aggcacaggt
     5161 ccggcggagg gcagcccaac ttgcagatag cctgtttatg cacaagcctt tgtaatttta
     5221 tccctcatca tcagtataaa ccattggatt ccattacaca cctggccctg ggcgcatgca
     5281 tgggtgaagc cttcgcaggt aaaaaactgg gcaggaaggc cggattgtgg ggagcattgg
     5341 cccagagcat tccggatatc gattttctgg cctctttctg gatggatacg gccagcaacc
     5401 tgttggcaca ccggggcttt acgcattcca tcctgttttg cgccctgatc actccggtgt
     5461 ttgccatcct tgcggaacga tggcaccgtc cgcacaacat cagcctgtcg cgctggctct
     5521 tgttttttgg cggggtgatt tttacccata tcctgattga tgccttcaac aattatgggg
     5581 tagggtggtt tgagcctttc agtcacctgc ggatgtcgtt caatgccatt tatgtggcgg
     5641 atcctttttt tgccctggga cccatccttg cggctgttgc gctgatctac ctgcctagga
     5701 caaatcttct gaggcgtccg gtatggctgt ttggactggg gctcagcggg ctttacctcc
     5761 tgtattgcct ggccaacaaa tattatatcg accgaaccgt aagggatatc ctggcgaatc
     5821 aaaagatttc ctacacccgt tattttacca ccccggcgcc gctgcaaaac tggttgtggt
     5881 atgtggtagc cggagatgac cgcgggtatc atgttgggtt ccggtcggtg tttgaggacc
     5941 gcccgaccat ggactttgaa tattttccgg tcaacgattc gctcctggac gaaacgcatg
     6001 accatgtgga attaaacaaa ctcaagcgtt tctcccaggg tttttacacc gtagaaaaat
     6061 ggggcgatac cctcgtcttt aatgacttgc gttttggaca gatcatcggg tggcaggatc
     6121 ccaggcaaaa atttgttttc cattacttcc tgcagcatcc cgacgacaat accctggtcg
     6181 tccagcgggg caggtttaaa cactgggatt gggcggtagc ccggaacctc ctggtgagga
     6241 tattcaggga ttaagcatcc ctttattttt tctgctggta atagaaagtg aacaggtaaa
     6301 aaaccacccg gataaactca aacgaaagcc acctcccaaa ctggatgaat acgttttttg
     6361 attttttgtg tttttccagc gtgatccgga cacaatcttc agctatgatc ttatccataa
     6421 gtttcagcat atcctgggtc atggctggct ggatgacatc caggttcaat tcgacactgg
     6481 cgtaggcgct gatgtcgttg atgttgtacg agcccagggt gaaccactca taatcgcata
     6541 ttcccagttt gccgtgcagg atatttttct ggtattcgta caattcaatc tcgttccgga
     6601 gcaaccagtc atacaaccac ctttcggcgt acttgaccag catgacatcg gaggcgccag
     6661 ccgaaatcac ccggatcctg accccccgct tcctggcttt ggacatcagg cggcgcaggg
     6721 tcttcccggg taaaaaataa ctgctcaaaa gggtaacctg tttgcgcgcc gtcctgaaca
     6781 tgtggtaata agtattggat atctggtttt tgccccttac ccaatcgttt ctccgcatcc
     6841 gtacataggc cctgcttcct tgcgggatct ccggcctggg tactttttca cagggggtaa
     6901 ggcccatttt caagggaaac cggttccagg ttttccagca caggatacac aattccacag
     6961 cgacctggcc ttcggcaaaa agcgcgaaat ccagccaggc gggctggccg ggcaggtcat
     7021 tgtaccggtt gctgatattc agtcctccga tcatggcccg ggtggtgtcc actacaaaga
     7081 ttttatgatg aaggcgcctt ccaaagtaga aatacttgct cttgagcagg ggttcaaaga
     7141 atttaaacat tatgccctct tccttcatcc ggtggatcga agcgtcggga aaaccctggg
     7201 aggcataccc gtccgccagg atgtagactt ttaccccccg gcggcttgcc cggatcaggg
     7261 gttcgatgat catccgcccg gtttggtcgt cttcaatgat ataatattgg aggtggatcg
     7321 tatcgctggc ttcgccgatc agttgctcca tgagcttgaa aaaggcgctt cctccccgta
     7381 taaactggac cctgttgtta ttggaataag accggtctgg tgaaagatac atggatgatc
     7441 ggcttgaacg gcggtaaaaa taggaatatg ccaccattct gaaattcctg gacccgggaa
     7501 gtcagaaccg cccatccctg acctcaatca gttttccggc tgatccgctt catagctgcc
     7561 tatccgctta tcttttatat ttgattaaat gatataagga aattccaaat atcagaatga
     7621 ttaaagtagt caacgccggt tcgtgaaccg gtagaaatgc ttaatcccag gagatgggta
     7681 ctacccgtct cctttttttt atcccggcat ggactgaagc atcctgaaat attcgatgac
     7741 ttcctcgtcc gagacctggt caaaatcttt gtaaaaagcc ccaaccccat aaaactcctc
     7801 gggtacggaa atgcaaatca attcatcggt ttccggtttg agtttgcgga ctgcgtttgt
     7861 ggatgcaacc ggtaccgcaa tcactatttt ggaagggttg ccctttctca agacctgtaa
     7921 ggtagccatc agggtattcc ccgtggcaat cccgtcatcc actacgatca cggtcttccc
     7981 cttaatatcc ttcacttcct tgcctcccat gaattttgaa cgcatttcgt ccaatcgttt
     8041 cctgatcctc tgcacctccg cagtaacgta atcctggctt accttttcaa agggaacgat
     8101 aaaataatcg gtaaggctgg ctgcgccaat ggcgtattcc ttattgttgg gatgccctat
     8161 ttttttgatc agagccagtt ccatgggcaa tccgagagat ttagcgattt caaaggcaac
     8221 gggtactccg ccgcgcggga tggccatcag gacggcatcc tcttcccggt atttccacaa
     8281 tttggctgcc aggatggtcc cggcttccga tcggtttgca aagatcatgg cgatggatta
     8341 agggtttgat tgaataagcc aggcatcgaa ccagccgtag gccaattcgc ataccttttc
     8401 cagggtgccg ggttcttcaa aaagatgggt ggctcccgga atgatttcca tttttttctc
     8461 tccacccaat aagttgaaag cgtattggtt cagttctatc acttcagggt cccggcttcc
     8521 gatcaataac agggtggggg aggtcacttt ctgcagggag gaacccgcca ggtcgggcct
     8581 tcctccccgg cttacgactg cccctatttc tttcattccc gctgcggcga tcagggccga
     8641 agcggccccg gtactggccc cgaaataacc gatcctgcaa tccagggtga cggggttgta
     8701 aacaatccac cgggtggcat cttttaagcg ctcagccaac agaggaatgt tgaaacgatt
     8761 ttcataattg aggtcctctt ccaccgtaag caggtcaaaa agcagggtgg cgatatgccg
     8821 tttattcaga tatcgggcaa ccattttgtt tcttggactg tgcctgctgc ttccgcttcc
     8881 gtgtgaaaat atgactattg cttttgcatc cacaggtaca atcaaatctc ccttcaattc
     8941 aactttaccc gaagggatga tgacttcatc ttcaaattgc aattccatat aaatcccttt
     9001 ttgtgaagat aactgaattc tgttttcagc agtgtgaccg gaatcagggg ggggattgat
     9061 tcaattcatg ggaagcccgc ttgattgatg accaactttg aatgaatatg ggcagatatt
     9121 tttccaaacc gctgaaaatt tccggggaag ataccgtatc ggtaatcaaa caatggtcct
     9181 atcccttgcc cggtccttcc gccctggacc ccttgattaa aaatataggg gatgcaggga
     9241 tagtcatgct gggagaggct tcgcacggga ccagcgattt ttacctttgg cggtggcaca
     9301 tcacgcggcg gctgattgaa gaaaaagggt tcaattttat tgccgtggag ggcgattggc
     9361 cggattgtta ccggctcaac cggtttatta aaaatgatcc aaaagccggc aaagatgcgt
     9421 atgccgtgtt gaatgcattc aatcgatggc ctacctggat gtgggccaac tgggaactgg
     9481 tcgcttttgc cggctggctt caccatttca acaaggaaaa gccctccaac cggaaggtgg
     9541 ggttttacgg cctggatgta tacagcctcc atgaaagtct tcaggaaatc ctggcatatc
     9601 tccacaaaac cgatccctct gcctatgaag tagcccttga agcggcccgc tgttttgaac
     9661 cttaccgtaa aaatgaaggc ttggcctacg cccgggcggc tgaactggtt cccgagctat
     9721 gtcagacgga agtagtcagt ctgctcactg aaatccgggc caggatcact tcctacaaca
     9781 cggacccgga gaatgtcttc agtgtggcgc agaattccac cgtggtggcc aatgccgaac
     9841 aatattaccg ggaaatgatc cggggagggg cgcacagctg gaatctcagg gaccggcaca
     9901 tgcatgacac gctcgttaaa ttattggatt ttcatgggtc cgaagccaag gccgtgatct
     9961 gggcgcacaa tacgcatatc ggggatgcga gcgcgaccga tatgatccag gaaggcatgt
    10021 acaatatcgg tgaactcgcg cggctccatc tctgggacaa aggggtgtat ttggtgggtt
    10081 tcggcagcta tcaaggcacg gtcaccgccg gccatcgctg gggcggagaa atgaaaatta
    10141 tggacctgcc tccggggcgg agggggagtt gggaagacta ccttcatacg gcgtctcctg
    10201 aggataaact cctgctcatg gaagatttta aggacgatat tttgatggag catcacctcc
    10261 cacaccgtgc agtcggggta gtgtatcagc cggaatacga acagtatgga aactatgtgc
    10321 ccagtatttt acccttgcgt tatgatgcgt ttattcatat tgacgaatcc agggccctgc
    10381 atcccctgca catcaaacct tcgggggggc aagtgccgga gacttatccg ttcggggtat
    10441 aaatatttaa aatgccaaga agtatgagca atacggtaaa aataatcctt ctggtcttgt
    10501 tgggatttgt agcatccaca tctgtttttt cgggcttggc catttgccta aaccctgatg
    10561 gttccaccct gcaactcgat cctgaggtgc tccggcacac cgtattccgt gattttatga
    10621 ttcccgggct gatactcctg attattgtgg ggggatccag tcttgccggg gcgctctcca
    10681 ttgtttttga ccacccatcc tcccagggtt gggtgctggc tgcaggaata atcctggtcg
    10741 gttggattgc agggcaggtg gttttgatca ggacctatca cttcctgcaa ctggtttatc
    10801 tgattacggg tattttggtc gcaggtcttt ctttttttat caagcccacc actgaaattt
    10861 acagggtata gccgggtcag cacttattga acggatccgg tcaagggcgg cggctgatca
    10921 ggatattcaa ggctttccat tccggaatat aatcgctcac gattttgagc atgccgataa
    10981 acggaatgaa aaggatcatg ccgctgatcc cccacaggat cccacccgca ataatcgcaa
    11041 cgagtgtagc ccaggtgctg atattgagct gggagcccac tacccgggga aaaataacgt
    11101 ttccttcgag gtattgaacg aatgaaaata cggcaatgac ccccagcggg tacaggatat
    11161 tccccttggt cagccaggcc atggaaatgg gcaacatgga actgacaaaa atgccgatat
    11221 acgggataat ggtcatgata gcagtaaggt aaccaaacag cagggcgtgt ttgatgccga
    11281 gcaggagcag ccccaggctg ttgagggtgc cgacgatgag gtataccttg atcatgcctt
    11341 tgataaaatt ggcatatgtg gtcacggttt cccggaggat gactgagagt ttgtccttgt
    11401 attcttctcc ggccagggaa ctcagaaatt gcacaaacac tttccggttg taaaggaaaa
    11461 gggcggtgaa gaccggggtg atgtaggcga tatatatcgt gttgagggtg gccgacatgg
    11521 tgttgcggat cgcactccct gaaaagacgc tgggggaaga tttgaaatca ctccaccatt
    11581 ggtcctgaac cgcctgggaa atgccggtag tcgtttggat ccaggcctgg agggcaggga
    11641 tattggccag aaaccgggct ttcagttccg ggaagtcttg ctgtagggct ttgatctgga
    11701 ggatcagcag gaagcccagg aaaaggatta ataaagtcac gatgatcatg ccgaggacaa
    11761 tagccacggt cttgttccat ccccttgcct ccaggcccct gcaaaaggga taaaggacca
    11821 gggcgatcag caatccataa aacatcggga taaaaagggt cctgcccgcg tataacagga
    11881 agagcaccga cagcaaaagg gcggtgtaat aaaaaaactg ctggagtttg atttctttgg
    11941 actccatatg gctctggatc tggagaccgg cttaattttc ggtccttcag ggaccaatat
    12001 actatttggg atttgcagat cataaccgga tcaatattct tcgctatttt gtaccattgt
    12061 ttctccaatg ggcctgatcc tttttaagct cctggccggt atcgtaatct ttctgctggg
    12121 attcagtttc atcgaggaag ccatccggaa tgtcaccggg cgggctttca aacttttttt
    12181 aaaaaaacat accagccagc cggtcagagg gctcgtggcc ggggccctgg tgacggccat
    12241 cctgcagagc agcagcatcg tcaacttcct ggttttatcc tttgtgggaa ccggggtgct
    12301 caacctttat caggccctgg cggtcatgct gggggccaat ataggcacta ccgtcaccag
    12361 ttggatcatc gttctggttg ggttcaaact taatctggaa gacctggcct ttcccctgat
    12421 cgcgctgtgc gggtttttta cctttttata caaaaagcag aatatcctct ttttctggat
    12481 gaggctgttg tttggcatgg gttttttatt ctttggcctg gaatacataa aaatcagtgt
    12541 attcgaagtg atcgcatctt ttgaccttgc ttttttaaac cagtcgcccc tccttttatt
    12601 tgtcctgttt ggcttgattg ccaccaccct gacccagtcc agttcggcga ccgtggtgct
    12661 ggcactcagc gccctcaatg ccgaggcgat ctccctgatt gcggcgatgg ccatcgtgct
    12721 cggctcagag gtaggcacta cgctgaaact ggtactggct gccctggacg ggccggtcat
    12781 caagaaaaag gtggctctgg gcaacctggt ttttaattcg gtgaccctgg tcgtggcttt
    12841 taccttgatc aggccaacgc atagcctgat ccgggacttc atcggcatcg gcgaccacct
    12901 tgttgccctg gtcttttatc agagcctgat caatgtaggt tgcgcgattt tgttcttacc
    12961 catcctgaaa aaactgggtc actggctgga agtgctttct tttagggaca gtgacgatac
    13021 cagcatcatc cgcaaagtaa aggtcacgga cggagattat gcccatgaag cgctggaaac
    13081 cgaaacacac cagatgatgc aatgggtcct cgttttcacc aggaaggcgt tccgcctgga
    13141 aaatccggcg gagccattgc cgggaatctc ctccaaaatg gccggcatgg accgtttgca
    13201 atggtataac cacctcaaaa gcctgcacgg ggaaatacgc aattatgcct tcaacctgca
    13261 aaggctgacg actgaggagg aaaaactgaa tcgcatcgat caactgctgg agacggtgcg
    13321 ggatgccatg tttgccgtga aaagcataaa ggattcattt cacgacatcg acctcctgag
    13381 caattcttcc aacgatgtca aatttggatt ctatatggaa acccggcaaa aggtcatgga
    13441 actggttgac gagataggag gcattcttca gcacccggaa gcccagccgg tttatgatca
    13501 gttgttttca aatgtgaaga agctcgaaga ccattatcat accatggtcg gccggtttta
    13561 taaagacggc tttgacatcg ccttgtccgg ggatgaattt tcaaccctga tcaatttcaa
    13621 ccgcgaagtg ttcaatgccg gtaaatacct gattatcatg ttgaaagaat actatttatc
    13681 cgcgcagcag gctgaattgt ttgatgagca gctgcatgcg ggtctttgaa gaccagagga
    13741 ttacagccgt tgtattgtgg atgaatttta tcaccaggta gtatgacttc catcaggaag
    13801 ggtctttagc aaaaaaatta gttttgttta aaacctatat tatgaacgtt aaaaattatc
    13861 ctgaaatcac cgaaatcgtc gagcataaaa attatcttcc gagtatttcg gtcattttcc
    13921 cattcaacat caaagtggga atgggggtcg aactgaagca taaattgaaa accaaaaggg
    13981 atgaaataga gaaagaactc ctcaaatcct atccggcaga cgtagtggct tccctgatgt
    14041 ccaagtatac cagcctcacg gaaaacctga aaccgcaaaa catgcatcag ggtctggcca
    14101 tttttttgtc cccctggttc cagaaaacgt atttccttga ttttgagccc caggaaaaaa
    14161 acatcattga cagcagtttt gaaatccggg atttgatcta taataagaat gcagtgagca
    14221 aggccatcct tttattgctc agcgccaatg aaatccggtt ttacctcgtt tccccggcac
    14281 acgtgacgaa attgaatgtg gatatttcca accgcatcga ggcctttatg gccaatacct
    14341 tgcacgatgt ggaaattcac cacgaaaaaa gcgagcgcag ggataaagcc atgcagctct
    14401 ttcttcagaa tgcggacgag ggaattgccc tggctttgaa agaccatgcc tgtccggtga
    14461 tcgtggccgg aagcgaaaaa tacatcggtc attttaaaaa gatgacgaaa cacggggagg
    14521 tcattgaaga ttatatcgaa ggcaattacc ttgaaagtac ccctgccgag ttgaaccaat
    14581 tggccaggga atcgtttggg cggatccagg aaacccggtc ccggaaaatc attgaccagg
    14641 tcgaagatgc cctgaataaa aaactgctgg caacggggct gaaggaagtg tggaaacaag
    14701 cgacggagaa gaatggccgc atcctggtag ttgaaaaaaa ttaccggaag cctgccgcgc
    14761 aaggggctgt cgatgaagaa attataacta ttggcctgga tcacgtccct tttccgatgg
    14821 aagatgccgt ggacgacgta atcgaaaaag tgatccttaa cggaggtatg gttcattttg
    14881 tggatgatgg cctgctgggg aaataccgcc ggatagccct catcacctat caccagtaat
    14941 tacaatttat taagaataga atcaggagcc gttgctttac cggtaacggc tctttttttt
    15001 gggatcagtc cttaatacgg tcaattgcgg gcggtacagg cccctgttga atagggttta
    15061 tgcgggctac cttgcggttc gcggctctct tatttcctgg taagccggag gaaagtaaaa
    15121 ccaaaggagg agaaagaagc ggtgagggag ggattcgaac cctcggtaca ggtttaagcc
    15181 cgtacgacgg tttagcaaac cgttgatttc agccactcat ccacctcacc ggaccatggg
    15241 ccaaattttg gaagggcaaa tttagtattt ttcggtggac aagtaaaatg aagccgatta
    15301 aaatgaaaat acctgtttgg cgatggattc ttaaggcgtt ttcttgcggt atacgggcga
    15361 tcattttcct gattcctggc ttttcatcag gccaggtaag gatcgacacc ctttatccct
    15421 cagcctggtc ggctaattct gtaaacgcgg tcattttccg taaacattcc ctcacgagca
    15481 atacccaata ccagtttacc tccttttatg ataccacggg gacggtagtt gtaggccgaa
    15541 ggtcccctgg atccgggtcc tgggaatgga aacgactgaa ttataaaggc agggcagggg
    15601 acgcacacaa ttgcatcagc attcaactcg atggccaggg atatctgcac ctggcctggg
    15661 atcaccacaa caaccggctg aattatatcc ggagttcggc accgggatcc ctggatttcg
    15721 aacccatgca ggccatgacc ggccatctgg aaaaccaggt ctcatacccg gaattttacc
    15781 gcctcccgga tgggaatctg atattcctct accgggatgg cagttccgga aacggcaacc
    15841 tcgtgatcaa ccgatacgat tgcgcttcca gaacctggag ccgtctccac caggtactca
    15901 ttgatggtga aggggaacgg aacgcctatt ggcaggcggc ggtggacaac aagggtggcc
    15961 tgcatctgtc ctggacctgg agggagaccc cggatgtggc gagtaaccat gacctgtgtt
    16021 atgcggtctc ccgcgatggg gggctgcact gggagaattc tttgggacaa gcctaccggc
    16081 tcccaattac cgccgcgacg gccgaataca tccgccggat tccccagggc agcgaactga
    16141 tcaatcagac ctctatgacc tgcgatggct caggcaggcc atttgtggcg agttattggc
    16201 aagacccggg cgaagaggcg cctcaatacc atctcgtctt taaaagaggc aaaaagtgga
    16261 tttccagttc cggagggttt cggaagaccg gttttagcct gaagggcacc ggcacccggt
    16321 cgataccgat ttccaggccc caggtcctgg tatggggcaa agggaaaaca ttccgggccg
    16381 gcatcctctt cagggaccag gaaagggaca acaagccatc cctggccagc agcaaaggcc
    16441 ctggtttcag gaattggatg gtttccgatc tggatgaaga gtcgctcgac cgttgggagc
    16501 ccacatttga cccggacctt tggggttccg ccgggattct caacctgttt cttttgaagg
    16561 ttcgccaatt ggacgccgaa ggattgggca tggaccaggg atccccggtc cgcttgctcg
    16621 aatggcagcc cccgcaaaat caataatccc cctttttgag ggatcggaat gtggggccat
    16681 tagttataat ttcgcagtat ggtagaaacg ttggtcggca aatatccgtt atgcggccga
    16741 ttccatcccc cattgatcta tgactgaatt caatccaccc cggatggcct ttttttccga
    16801 aatttgcaaa aaacttaatc gaaaatcata tgaaaccttc aaggatcaaa agtaggctct
    16861 ggccggtcct cttcctggca ggatatgcct ggttgtcctc ggctcagtcc acttcaaacc
    16921 acgggaacaa atttgagcaa ttgggcacta ttctgcccac tccaaacgaa taccgttccg
    16981 caagcggagc ccccggccct aaatactggc aacaaagggt ggattacgac atcaaatgcg
    17041 aactggatga agaaaaccaa aagttgacgg gctccgaaac gatcacctat ttcaataatt
    17101 ctccggatgt gctaaactac ctttggctgc aattggatga aaaccagcac agttcggtga
    17161 ataatgccaa ttaccagaat accagcaata tgccccaggt cctgacggac cgggtgattg
    17221 accgggtcga ggaatacaaa acggacaacg ggtacgggca tatcattcac aagatcgtgg
    17281 atgccacggg caaaccgctg gtgtacacca tcaataaaac catgatgaaa gtcaatatgc
    17341 ctgttccgct gaagcccaac agtgtttttg tgtttaaaat cgactggagt tataaactgg
    17401 tggatcgcat ggatttcaac cgctttcaga atgcacgcgg cggttatgaa tatttccagg
    17461 aagacggcaa ccatattttt accattgcac agtggtaccc ccggctttgt aaatacggcg
    17521 acgaaattgg ctggcaaaac caccagttta ccggaagagg ggagtttacg gtatcctttg
    17581 gcaattttaa ggttcaaatg accgttcctg ctgatcacgt ggtgggttcc accggggaat
    17641 gcctgaatta tacgacgaat ctcaccccta cgcaaaagct tcgctggcaa aaggcccaga
    17701 ccgccaagga accggtgcag attgtcactc ttgacgaagc gctggctgcc tccaaggaca
    17761 aaagcaaagc aaaaaaaacc tggattttca aagccgacaa tgtccgcgat ttcgcctgga
    17821 cctcttcccg gcggtttgtc tgggacgccc tgcccatcca ggtcaataat aaaaaagtga
    17881 tgtgcatgag tttctatgcc aaggaagcct acccgattta tagtaaatat tccaccaaag
    17941 cggtggccca taccatcaag acctattccg actttaccat cccttatcct tacccggtag
    18001 cccagagtgt ggaagcgtcc aatggaatgg agtatcccat gatctgtttc aacccgggca
    18061 gggctgaaaa agacgggacc tattccgaag gcaataaaaa cgctgccatc ctggtcatta
    18121 ttcacgaggt cggccataat tttttcccca tgatcgtcaa cagcgacgaa cgtcagtgga
    18181 cctggatgga cgaagggctg aactcctata tgcaatacat cacccaggag ctctgggaca
    18241 ataaatttcc ttccgacggg gggccgccac ataccattgt ggactatatg aaatggccca
    18301 aagacaggct ggagcccatt atgaccaatt cagaaaacat cattggtttc gggcccaatg
    18361 cctacagcaa ggtcgctacc ggactgaata tcctcaggga aaccatcatg ggcagggaat
    18421 tgtttgacca ggcctttaag gaatatgccc gccggtgggc tttcaaacat cccaccccgg
    18481 ctgatttttt ccgcactatg gaagatgcca gcggggaaga cctggactgg ttctggagag
    18541 gctggtttta cagcattgat gccgcagaca tatccatcga taccgttaaa tatgccaccg
    18601 cagatgtaca tgccgttcct tccaggttga cggatacgat ggtgatgagg cccctggcca
    18661 aacccgatgt gaatgatttt gaggaccttt caaaaatcag aaatcgtcag gataccaata
    18721 tcgtctttgt gaccgaccgg gatacgtccc tgcgagactt ctactggaag tatgccaggg
    18781 atatcgccaa atacgacacc accaaatacc cggtgaccct ggtagccaac caggataccc
    18841 tcgacgccga ggcgaagaag aaatacggca acaaacatat gtatgaaatc acgttcagca
    18901 ataaaggagg actggtcatg cccattatcc tggaatggaa atacaaggac ggcagttctg
    18961 agattcagcg tattcccgcc caggtctggc gctacaatga aaataagatt accaaggctt
    19021 tcctgaaaga caaaaagata gccaacatca aacttgatcc ttaccgcgaa acggccgata
    19081 tcgacgaaag caacaacgtg tacggagaag aaaaaccgat accggaaccg agccgtttta
    19141 aagtattcaa acaaaaacaa gtgactcctg cgcccggcgt caacccgatg caaaaggcgg
    19201 cggagaaagc gaaaacctga ttaattacaa tttatttgaa agcctcgatg gatatcccga
    19261 atatctgctg aggctttttt tttatttaaa aattccaatt cacacctcat gaaattcaat
    19321 gttttcattt ttatcctggc cgcgtcctgg ttgcagcggc ttccggccca aaccgatctg
    19381 gagacgatcc ggcaaatcca ggaagtgtgc acgtattatc tcgacggcgg caccctgggg
    19441 gacagcctcc ttttttcgaa agcctttgta ccggacgggc agatgaggta tatccgaaac
    19501 gatacgctga tgaacgtatc gctcggggat tttatggccc gtgcccgcaa caacggggtc
    19561 aggcaaaacc ggaaaacaaa gattgaatcc atccaggtat acggcaatgc ggccacggcc
    19621 aaactgacca ttgaataccc gtctttttat tttcatgacc tcatgagcct ccttaaaacc
    19681 agcgagggat ggaaaattgt tggaaagatt ttttaccggg aagaaaaatc ccctcctgtt
    19741 aattaggttg cccggaccgt ccggactccg aaaacccggc ctgccgtctt gccctggttg
    19801 gcctcgatcc gcaccaggat ctctgatccc atattcaaag ctggagggag atcataaaat
    19861 acatcgtaaa attttccgga atggccgccg ggccactcca cggtcgttaa tttctgaccg
    19921 tttaccagaa tatcccagga cctgttttga tcgtctccca ggtaggtaaa gaccagctga
    19981 gtgggcaggc cgggatgggt tttcatccgg aactcgaaat aatgcccggt acgggcttcc
    20041 ctcccggtgc ggcccaaagc cgcgtccaca taagagcgct cactatgact caatccgtgc
    20101 ctttcttcgt cctcctcatt acccggctgg ataaaatcga tggtgcgctc ctccagggcc
    20161 ttctttaggt ccagtgccgc ctggtagtcc cggagcctct gcacatattg ggcttcggtg
    20221 taaaaatcaa aatatacagt ataatgttgg tcgtaaacct ggtaaaaggg tttcagcatt
    20281 acgggggaag gcctggtgcc ggtcaggctg aaggtcaacg cttggtttcc tggtgcaatc
    20341 caatggtttg ggtccctttc tttcgagacc agcaccggaa tttccggctc cgggggactg
    20401 gaatcaccca gcagaccggc aagcaccagg gggccgtatt ttatggcaat ccggtcggcc
    20461 tgatccggca taggttcggt ataaagagtc ttttccaaag agatccggat ccggtcccct
    20521 tttttccagg gatgctccag gaaaaggtat ccgtttcgca tgtactgctt aagggctggt
    20581 tgttgattta tggtgatatt tatttttttc gaccaaaaag ggtgacggat ggcaagggtg
    20641 gtgccgggcc ttggatttga tctcaaccaa aaatcgactt catcccgggc cgggaaaagg
    20701 gtttgttgtt caagcaggag tttccgggat ttccaatgca cggaagaagg gatgaagagg
    20761 tttacataca ccaccttaga cccgtcgtgg ctgtatatct gttcattgta tttcgaatgg
    20821 ttttccatgc cgctgcccac acagcaggtg aaagtattga aggtatcgct gaatgttttt
    20881 ttagcgcccg ttcggagggg gacgaaataa cacatcatcc cgttctccgg gttttgggaa
    20941 gccaggatgt ggttgtacaa ggccctttca taatagtcca tatacctgga atccgggctc
    21001 agggcaaaaa agtgtcgcgt gagtttgagc atgttgtacg tattgcaggt ttcacaggta
    21061 ttgtcactga gtcgatcata caaacggtcc ggctccccgc agtattcata attgctgttt
    21121 cctccaatga cgtagctgtg atggttcacc atggcattcc agaaaacgtc ggcgatccgg
    21181 tagtcccggg gattggccgt gagttcatat tgccggacgc ttccgatcac tttgggtacg
    21241 ttggtattgg catgtttacc gggcatggga tctatgccct gggccagatt gcccaaaaca
    21301 aattcatcat aaaacaggta agaaaggtcc aggtattttt tatttccggt gaaagcataa
    21361 agattggcca gtacttcatt catgcctccg tattcacaat cgagcatccg gagccttgcg
    21421 gaatcaggga gatgggcgat ggtttgatgc acccagtccg ccatccggct gacaatctcc
    21481 agagcctccc ggttgcccgc atggatataa gcatctccca gcccggccat gattttatgc
    21541 accgtatacc aggggctcca ggctccgttg aggtcaaagc ccgtggcccg gatcatgcct
    21601 ttggacactt tcaaaaacac ggaatcttcg ttgggaatgg ctccgagata accggttttc
    21661 ctggttgtct gaattcggcc aagttccttg atggtatagt ctattttttt cttcagggcc
    21721 cggtcgccgg tagacgcata catcatggcg gccgctgaca aataatgtcc cagggtatgg
    21781 cccgataatc ctccgctttc ccagccgccg tatatggaat ctttgggagc aagtcctgca
    21841 tttttgtgaa aacggtgaag cagccggtcc ggggaaagca ggctgagata aatgctgtcc
    21901 atcctccgcg catggtaaaa gggactcccc ggcaggaggc ttacctcctt aaggttgaat
    21961 gcatagcttt ttatgggcac gatgggattt acccggatgt tccggtttcc cagttccggt
    22021 actaacgatt gtccgggtac gggaggaaaa tacccaacca agaaaatgca aagcatgacc
    22081 aaagaacgaa ataccatccc gccaaaatag agaaaagttg tgtacccctc gttcccttat
    22141 cgccgactat tgttagtact gactactgac tactgactac tgactactga ctattgacta
    22201 ttgactattg actattggtc actacaccgc catactcatc ttgatcttct tctccagttc
    22261 cgataccgtg gaacggaacc catcatccgt atccaggagg tctttgatgg tttttaccga
    22321 atagatgacg gtcgaatggt ccctgccgcc aaaggtgccg ccgatcttgg ccagcgaata
    22381 ggtggtcagg tttttcgcga ggtacatgga caattggcgg gcgatgacca cgctgcgttt
    22441 tcgggttctg ccctgaaggt attcttcctt cagattaaaa tgttcggcca ccattttctg
    22501 gatctggtcc agggtaatct cccgggtata attgcgtaca aatttttcaa taacctcttt
    22561 ggccagcagc acatcgatct gcctctggtt tagggaagcc tgggcggcga gggaaatgag
    22621 gacgccctcc agttcgcgga cgctttcctt gatattgtgg cagatcagtt ccagcacggg
    22681 atagggcaac tggatttctt caagttgtga tttcgcgtcc aggatagcca tgcgggtttc
    22741 gaaatcgggc gcctggatat ctgcgctcag tccccatttg aagcgggaca ccagcctttc
    22801 ttcgacatcc tggagatcct tgggagggcg gtcggagctg atgatgatct gtttcccgac
    22861 ctggtgcata ttgttgaaaa tatggaaaaa gatttcctgg gttttggtcc ggttggccag
    22921 gaactggata tcgtcgatga tcagcacgtc caggttgtgg aaaaaggcca taaaatcatc
    22981 gatggcattg ttttttatgg actgaatgac ctgctgggtg aatttttcgg aactgatgta
    23041 caatacattt ttatcgggat gattggccag gatttcgttg cctatggcct ggacgagatg
    23101 ggtcttgccc aaacccaccg cgccataaat gaagagcggg ttgaatgcag taccgccggg
    23161 tttgcgggcg atggcgatgc ctgcggccct tgccagtttg ttacaatccc cctctacata
    23221 attggacatc cggtaatcgg ggtggaggtt ggattccacc ttggccttgc ggatccccgg
    23281 gatcacaaaa ggattgacaa tatggctgga atccaggggc ttaggaaaac tgggttcccc
    23341 ggcggaactg cgggtctggt tttccatagg gataaaatat tcaagccgtc cctgcttgcc
    23401 gatctcatgc cggatgcatt ttttgagtat gccgaggtaa tgctcctcga tccattcgta
    23461 aaaaaacttg ttgggtacct ggatggtcaa tacatcgtct tccagtttga tcggtttcac
    23521 gggttcaaac caggttttga aactttgctc cggaattgca tcgcgtatag ctttgagaca
    23581 attattccag acgatttgat gcataaggag catataagat actagaattg aaatttaaga
    23641 agatgatccg caccggattt tttgctcccg gcctgttttt ttattcggat gagtgaatgg
    23701 atccgtaaat aaccgggcag gaagcggcag aaatcctttt caacaaatgt caaaaaaata
    23761 tgccaaacgt ttcggtattc aggcgggggt caaaacatat tttcttcaac cattcataat
    23821 ccattcatta ctacaagttt tacagcggtg cctggtttta tttctttcaa actcaatgtc
    23881 gatccggccc agcaacacgc ctccccagcc aacctggttg ataatcaatg ggtttcctcc
    23941 aaggtcttta accacttgcg ctttgtcgag aaaggtatgg gtatgtccgc cgaggatcag
    24001 gtccacatga ttcaacaggg gggcgaggcg gaggtccgat attttttcgt tccggtactg
    24061 gtaacccagg tgggagaggc aaatgacgta atcgcatttg atttcctctt tcagcaaaag
    24121 gcttatttga ttggccttgt tcacgggatc caggtaccgc gtattcccca ccagggcgtc
    24181 gggaatcagc cctttcattt ctatgcctaa gccgaaaatt ccaaccctga ggccatccaa
    24241 ctctttgaca aaatagtctt tgacctggcc ttccatcggg gtgtccctga aatcatagtt
    24301 tgaaaccaca agggggaagc gggcatgagg gagttgggtt ttcagcattt caaggccccc
    24361 gtcaaaatcg tggttgccca tggtggcggc atcatatccc atggccgtca tggttttcat
    24421 ctcgatttcc cccttgtaca ggttgaaata gggcgtcccc tggaagatat cccccgagtc
    24481 cagcaacagc acgttttttt cctccgctct gatctttttg atcatggcag ccctcgcggc
    24541 agcccctccc atgccctgta gacggcttcc gtccatcgga aatggatcga tccggctgtg
    24601 aacatcattg gtatgcagga tggtgagctt tactttttct tcgggcgccc atgacctcca
    24661 tggaattccc tggccaagca gccagccgcc cccaagcccg gattgcagca aaaagtccct
    24721 tcgtttcatg ttcattattt ttttatggtt atgcgctgat cgaggtttgc ccggaggatt
    24781 cctttttcac gggcttccgc agtcagtaaa tcacggataa ggatgttttt ttcaattctc
    24841 ggcttatcct ttaaaaattc gaggttgtcg cccccgttgg cgatgtaatc ggggaaaacg
    24901 acatggtaaa ggccttccgg gtcaataccc tttccctgga tcctgatatc ggtcgctttt
    24961 ttgttttcaa tgcggaaacg gacctgggag gaaaccggtg cgccgccggc ttcggcgaca
    25021 tagtccagga acctctgtac ttccgttcct tttaattgaa gcaggaccac caggttgtca
    25081 aaaggcatga tttcaaacat ttgagaaagg gagacctccc ctttacccag gttgtttatc
    25141 cttattcctc cgtaattctg gaatgcaaat gccggggttt cggtataaag ttttgaagcc
    25201 tggttataca gggcgtccac cacccagttg gtcaaggtgg attccggtcg ttctttggtc
    25261 atggactccg gagcggtagc gatgacctca ttcatttttg catccaactc ggatttgtaa
    25321 ggaagtataa gcctgctgac ctggctgtcg ggagcggccg atgacacgcg gagggtgtct
    25381 gccctgacca tagccaagtg atcgtaggtc ttgcattgtg caaggagcat cccggctcct
    25441 gccaacagga tgtatttaaa ctttgacatg gagtttggct tcatttcgga tggcttgaag
    25501 agctttattc agtgaactgt tttgacgctg ttccatcagt ttctgggtca gggttccgct
    25561 cccggtttcg ggagaaagtt cgccggcagt atggatgatt tcatagatcg cctcgtcccg
    25621 ggccaggagg atgatgttcc acaatttttc tgaaatatag atttgctggc tgatgttatg
    25681 gtcaaactcc tgttgtatgg caacgatcat ggcctgactg agggcctggg cgttgagctg
    25741 gggagtttgg aggcgcatgg ccaggggacc gggctggatg cgttcgcaaa gcagggccag
    25801 ccgctcataa gcattaagga tcaggccgga ctggcttcca ggtacccggt ccaggctttc
    25861 cctgatataa atgcccaggt tccttttcaa aaacaggtaa ataaaaagca acacggcccc
    25921 gataaaaaac agaccggcag ggactaaaat cgaggagaac ttgcctgaaa acatccgggt
    25981 atggattttg cgggcaaaat taacattata ataccaataa ggcccggccc ggtcaaggga
    26041 ggccaggccg cgagggcatt gccagatttt aggagtcgga ttgaaccatt gccctttggt
    26101 tcatgtttaa aaagaactaa ttttggatga aaatattcag agcattatga atgatctcga
    26161 gaccgaaacc cttcccttga ccctgagcga tagcgcagtc ggccagttga agcgcattcg
    26221 agaagaacaa cagatcccgg aagacctggg cctgcgggtc ggagtgaaag gtgggggttg
    26281 ttccggtttc tcctatgtgc tgggatttga ccagggccag gaggacgata tgcgattttc
    26341 gacccagggc ctggagatcc tgatgaataa ggcccatgcc ctttacctga tcgggatcca
    26401 gatagactgg gtggacgggt tgaacaacag gggctttacc tttaccaatc cgaatgctaa
    26461 aagcacctgt gggtgtggca cttcgttttc ggcctgattt ttattttccg gttttaagtt
    26521 tttttctttt tttaacgacc tcctggctga gttccagttt gtaaatctct atttttttcc
    26581 gcagccggat attgcctgcg gccaggatct gggcggccag gattccggca ttctttgccg
    26641 cgtttaaagc taccgtggcg acggggaccc cgttgggcat ctgaagaatc gacaagatgg
    26701 aatcccatcc gtcgatcgaa ttgctggatt tgatcggtac ccctatgacc ggaagcgggg
    26761 ataaggaagc caccataccg ggtaaatgag cagcaccccc cgcgcccgca atgatgaccc
    26821 ggatgccccg ggtatgggcc gaggtagcaa aatcaaacag gtattttggt gtccggtggg
    26881 cggacacgat atctacttca aaaccgatcc cgaaatactc caggatatgg gctgcctctt
    26941 tcatgaccgc ataatcagaa tccgatccca tgacgatgct aacctgtatc ttattcattt
    27001 tcaatatgga ttaaaggttt aacaaaagta agggatcaac tgatcacctt gagatggttt
    27061 cggacccagt cggccttttc cttcacgtcc tccctggttt tgccaaggat attcacatga
    27121 cccaactttc tccagggccg ggtctccttt ttcccgtata aatgcacata aacccccggt
    27181 aaagcaaggc aggaatcgat gccctggtat ttggccggac cgctgaagcc cggttcgccc
    27241 aataagttga tcaggaccgt gtgtccggtc acttgagtga ggcccagcgg aaggcccagg
    27301 atacaccgga gatgctggtg atattgggaa gtcaaattgc cctcaatggt gtgatggccg
    27361 ctgttgtgag gcctgggagc cacctcgttg accaaaacct gatcgttttc atcaatcagg
    27421 aattccacgg ccagcagccc aatgattttc atggaatgga tcaggtcggc ggcgatttcc
    27481 ctggccctgg aagctgcagc cgggctgatg cttgccgggc tttcctggta gagcaataaa
    27541 ttggcctcgg gatgaaagat catttcaacg ggttcataag tggccacctc gccatgggtg
    27601 ttgcgggcgg caagaaccgc tatttccgtt tgtacccgga ttttttcttc aatcaggctg
    27661 ggcccctcca ggagcaggtt taaatcggcg gggctgttta ccatcagcac cccccggcca
    27721 tcatagccgg aagtcctcag tttctgaaca aaaggcgtct gtaatttatt ttccctgacc
    27781 agattttcaa tctcttgcct ggatgaggtt aaactgaatt ctgaagtagg tatgccctgg
    27841 gattgcagaa actgcttctg gctgcctttg tctttaatga tccccagggc ctccgggccg
    27901 ggataaattc ggataccctt gcccgaaagg acttccaggg cttttgtgtt gacatcctct
    27961 atttcgatgg taatgatctt tttatctgcc ccaaacttta aaacggtttc atagtccctg
    28021 aaatcgccca gggtttgttt tgcacagcaa gcggcgggac aatcttccgc ggggtcgagc
    28081 acatggagat tcaggttcca gtccgcaccg gcctgggcaa gcattttacc caactggccg
    28141 ccgcccaaaa tgcctaccgg tgaggaatcg aaagctgaat ccattcagaa atggccaaaa
    28201 gtagatattt ttcgttttaa tttgaatgta tggccaagtt aaccctgatc cacaatgccc
    28261 gactggtcaa cgaaggaaag atccaggagc aggacgtgtt gatctccgcg ggcaggattg
    28321 agcatatcgg accggaacgt aaacatttgg cggccgaaga aatagatgcc gggggcatgg
    28381 tcctgatgcc cgggatcatc gatgaccagg tccatttcag ggagcccggc ctgacccaca
    28441 aggcagatat cgcctccgaa tcaagagccg gcttggccgg aggggtcacc agttttatgg
    28501 aaatgcccaa taccgtgcct cacgccacca cacgagaagt actggaaaaa aaatatggca
    28561 ttgcatcaga gcaggccttc agcaattatt ccttttacct gggagccacc gatcaaaacc
    28621 tggaagaaat caaacaggtg gatcccaagc gggtatgcgg gattaaaatt ttcatgggat
    28681 cgagcaccgg agacctgctt gtcgcggatg accgtgccct ggaagggatt ttccgcgaat
    28741 cgccaaccct gatcgccacc cattgcgagg acgaaaccat gttccgtcaa aagcaggaag
    28801 aataccgggt caaattttgt gatgagatcc cggcctcggc gcatgaattt atccgcagtg
    28861 cggaaggatg cctggcatcc agcagcaaag cggtgaacct ggcgcagcaa tacggaagca
    28921 ggctgcatat tctccacatc tcgaccctgg atgagctggc cctgttcaac aacgaccttc
    28981 ccctggagca aaaaaaaatc actgcggagg tttgtgtgca ccaccttttc tttgacagcg
    29041 ctcaatatgc cagcctgcac aacaaaataa aatgtaatcc ttccatcaaa catgcccgtc
    29101 accgcgaagc gctccggaaa gcccttcggg atggccggct ggatgtggtg gctacggatc
    29161 atgccccgca taccctttct gaaaaaaccg gcagttacct tcaatccgca agcggcctgc
    29221 ccctggtcca gcattccctg caaatcatgc tgaccctgca tcacgaggga gagatccccc
    29281 tggaggaaat cgtgaccaaa atgtgtcatc atcctgcgat ctgtttcaag gtcaaagacc
    29341 ggggattcat acgggaaggc tatcacgccg acctggtcct ggtcaatccg gacgaaagag
    29401 tcctggtcac ccgtgaaaat attttgtaca aatgcggttg gtctcccctt gaaaattacc
    29461 agttgagggg agaaatcctg gccacctggg tcaacggcaa cctcgcttat aatcggggag
    29521 tttttaatcc cgtgaaaaca gttcagaggc tggaattcga ccggtaattc agaggctgga
    29581 attcgaccgg taatgtgaag acaggggagg gaaagcaaac acctattaag gtccgcaccg
    29641 aatgcccagg cataccgttg attgttcctt gcattttacc cattcgctga tccggttgcc
    29701 gggccagacc cagatgatcc ggtcagcatc acagaggacc agggcttgtt ttttttctaa
    29761 tccactgacc ttcatatcgg tcaggaaatc ctggagcttt tttgttttac cctccatgcc
    29821 cgagggtgca aagcggtccc cggccttcca ggtccggagt cggagtggaa accggacaga
    29881 ggaaagatct gcctgggcaa aggacttcgg accctccccg gccgggcagg tgaatatctc
    29941 aatatgacgg tcgtccaggc gggtttcaaa gggcaggccg gggatttcaa tatccacggt
    30001 cgttgggggc tggctgactt cataaagttc tgccatttgt ccgatggtca tgagttcaaa
    30061 ccgttcagaa taaaagcggt tgcccttgtg gccttcgcga agggccattg cgatattgct
    30121 gcattggctg gagttaaaac cgtaatcttc aagtaaaaca aacaataaag cgggatccgc
    30181 cggctcaggg atggaaagaa ccacttttct tccagacctg gtaaggtgca atcgggacat
    30241 ttggtttttc cacagggtat aaacaggcag gtattgctgc actcgttctg aaaatgccgc
    30301 gaggtgggcc gaagccctgg gatttatttg ttccagcaaa gggatgatct ggtgtctgat
    30361 tttattccgg gtataatcgg ttttgagatt cgatagatct tcccggtaag gaatgccccg
    30421 gagggaaatg tattccctga tctctttttt agagacggcc aggagaggtc ggatgatcct
    30481 gccctgcatg ataggaattc ctccggctcc gcggagaccg gatccccgga ccaggttgaa
    30541 gatcagggtt tcggcctggt cgtcctggtg atgggccgtg gcaattttat gatacccgta
    30601 ctggtcggct atttcgctca accagtcata gcggagatcc cgggccgttt cctggatccc
    30661 tttccctgat ttctgcgcct cagcccgggt gtcaaaagaa acactgtgta caggcaggcc
    30721 gagagtggaa cagtgggcac ggaccagggc ttcatccagg tcggattctc caccccggag
    30781 tttaaagttg acgtgtccgg cggcaaggtc cagatcgagc gcttggagca gggatatcag
    30841 gcacatggaa tccatcccgc cgctgaccgc tacgaggatc cggtcgcccc gcgcaatcag
    30901 tgggtgggtc tccaggatgg attttatttt tctctcgatc gggaatggca tatttttgca
    30961 gcctcaatac ctgactatgt cgtttagcaa atttggcatt tttattttat tcgccgggct
    31021 ggcagccgca gcctgtaaga ataatgccga ccccacgggc gatgtttttt cacagaaaga
    31081 tacggccggg atcaacgaga tgccggtatc cgccaatgcg gtccgttcgg atacgcccat
    31141 ggtatccagt tttacaaaac ccgacgcgat ggcccggaac aaatacagcc gttttgacac
    31201 gattttgatc aaggatctat gcacccaaat gtggaaattt gacggtggga tcgaaggtcc
    31261 caagaacatc accgccgaag aaatgcaagg attctggatg cagttttttc ccaacgggaa
    31321 atatgaaaaa gggatgtacc ataagattac ctcaaagggc aattttacca tcgacaatct
    31381 cggttttctc gaaatgaccc cggacgattc gaaagaaaag aagagcgaat ggcagtccaa
    31441 attcaacagc gatatgctca tcctcatcgg gacggagaag taccgggaca atcatatcca
    31501 gatgcgcctg tcccgtattg gtaaaaagcc ggtaaaaacc aattgatttc cgccgggttc
    31561 aggatatggc cctggctttt gccggttttt tagctgccgg cttttttgaa ggggtgcgcg
    31621 tactttttgc ctttccggtt gttttacgct ttcctttctt ttcaaaggcc tggggatcgg
    31681 aggcaaggat cagggacttg acctgttcca ggctgtaggc cgctgcctct tccggagtaa
    31741 tcctctttcc atccggattc ttcggaaaat tataatgttt tttcccgtaa taaattaccg
    31801 gaccccatcg gccgttctgg acgcttaggt tttcttcttc ccaccggctg aggtatttac
    31861 tggcctcttt ttcctctttg gccttgacca ggtcaatggc tgttttcggg tcaaggttat
    31921 cgaaattgta tttcgcaggc acattgacaa agagctcgtt ccatttcaca aaagggccaa
    31981 atcggccttt gcctttggtg atgggttttt ctttataata agccaatggc gcaagttcgg
    32041 cccgtttggc ctcgatcagc tcaatggccc ggtccaggct taccgcaagc gggtcttcgg
    32101 gccggggaat ggaaatgaat ccttcgtcat actttacata ggggccaaac cggccttgac
    32161 caatgatcac ttccttgccc tggtattcgc ccagggaacg cggcagtttg aacaattcca
    32221 tggcatcttc gtaggaaatg ttttccatgg actgccccgg cttgaggttg gcgaattcgg
    32281 gtttttcatc ctctttcaat tcctccgtgt caccgatctg caccaccggg ccataacggg
    32341 acattctgac aaataccgcc cgtccggttt tgggatccga acccaggatc cgtttcccgc
    32401 tttcgcgacc cgtattctgc agggtgtctt ccagtttttg atggaatggc cggtaaaaag
    32461 cttggatcat ctgatgccat tccatattcc cttccgcaat gatgtcaaat tgtttttcaa
    32521 tttcggccgt gaacccgtag gtcatgatat cctcaaaatt tttagtgagg aaatccacga
    32581 ccagcatgcc catgtcagtg gggaacaggt tgtttttaga agccccggtg atttccgaat
    32641 ccatggattc ccggatctgg ccgttttcca gggtaaggat ttcgaacttt ctctcgatcc
    32701 cttcccgttg ttctttcacc acataacccc ggccggtttc cataattttg gatatggtgg
    32761 gggcgtaggt ggacggcctt ccgatcccga gttcttccag tttttttact aggcttgctt
    32821 ccgtgtacct ggaagggggc ctggtgaagc gttgcaccgc ttccattttt ctaaggtcca
    32881 ggacctggcc gatatgcagg ggaggcaaaa tggattcatc atgtccgtcc tcttcttcat
    32941 cgctggattc caggtaaact ttcagaaaac cgtcgaactt gagaatttcc ccttcagcct
    33001 ggaggtattc cccgggcagg gtggatattc cgattttcac gatggttttc tccagttcgg
    33061 catccgccat ctgggatgcg atggtgcgtt tccagatcaa ttcgtacaag cgctgctcat
    33121 cgcgaatagg ggatacctcc agccggttga tgtacgaagg acgaatggct tcatgcgcct
    33181 cctgggcatt ggcggctttg cttttgtaaa tcctggtttc cacataatgt ttcccataag
    33241 cccgctcgat ttcttcagtg atttgctgga cagcgacatt gctcaaagcg gtggaatcgg
    33301 tacgcatgta ggtgatgtag ccttgttcgt aaaggcgttg ggcggtcacc atggtgcggc
    33361 tgacggaaaa cccgagtttc cggctggcct cctgctgcag ggtggaagtg gtaaaggggg
    33421 ccgccggctt tctccgcaac ggtttaatgt caatggactg aatgctgaac cgcccggtga
    33481 tacaggcgtc caggaagctg cgggcgtcct ttttatcgtc aaatcgttcg ggccgttcgg
    33541 ctttcagcgt atacaccctg ccttccgcat cctggatcct gaaaagggcg ctgactttga
    33601 agaaagggac ggctacgaaa ttccggatct ctctttccct ttcgacaatc agccggaccg
    33661 cgaccgattg cacccggcct gcagacaggt tgttcttgat cttacgccag aggagttcag
    33721 acaattcgaa accaaccagg cggtccagca ctcttcttgc ctgctgcgca ttgaccaggt
    33781 caagatccaa gaggcgaggg cgaaggaccg cctcctgaat ggccggtttg gtgatttcat
    33841 ggaagacaat ccgtttggtg attttaggat cgagcccgag tacttcgcaa agatgccagg
    33901 ctatggcttc gccttcgcgg tcttcgtccg tcgccagcca gacttcttcg gctttccttg
    33961 cggtgtcctt taactccctg acgacttttt gtttttccgg gctgacgata taattgggct
    34021 gaaaattatt ctcgatatca atgccttttt tccctttctc cagatcccgg acatggccaa
    34081 aactggattt tacctggtag tcttcaccca ggaatttctc aatggttttg gctttggccg
    34141 gtgattctac aatcaacaat tttttaccca tactgattcg gtcccacttg attattagcc
    34201 tgcaaaacta taaatatttt atattttgcg attttagcca gcccttctat gcctgttttt
    34261 cataatatat ccggtatccc tggaccctgg aaaaccatgg cctctggacg gccgggcccg
    34321 ggtatatttg gaagctgaca gaaggagttt ttaatcacgc cacgggtgaa aaagcacagg
    34381 gataaaataa tcattacgct gctggtccag gcggtttttt atacactggt ctggatggtc
    34441 catgactaca cggcccttct tttaagcgcc ggaatcggaa tcatagccct tgccatactt
    34501 atcatttcct ggttgtccga ccggatggaa tacgggaata tgccatcctg gtattatccc
    34561 gttttgtgga tcagcatcct ggtcccgctc cttatctcca tcgtattctg gttgctgaag
    34621 gggggacaaa tggattggat ggagcccata ttttaatccc aaccgggacc actttcccgg
    34681 gaaacaaagt ccttattcct ccgtctgcgg aggcgtggtt ttcttcagag aaaggcgaaa
    34741 tggaaaattg tgcaggatgg tggccaatac caaaagtgaa tcctgggcag tttccaccat
    34801 ataggcggct tgtccggtca caatggcttc cctgctccat tggatcgcat aagatgaatc
    34861 cgatccatat agattgtgag tgagctcggt gtccgaaatc ttgaaaactg cgccgttgac
    34921 agtctgggtg atcctgttgt tcctggatgc ttccgtcacg atccaggttc cttttacctt
    34981 gtttttatca aataggggag gctcggactt gcaccccaaa atcaccccaa accatacccc
    35041 caacagcaat attctcatat tctcttttcc attaaccggg agcaaattta tatgcatcgt
    35101 gcttaaaggt caaccggttc cggttatatc gccgccgtca gttccagcaa ggtgatctcc
    35161 ggaaggatgc ccacccggcc tgggtaaccc aggaaaccaa aaccccggtt gacataaaga
    35221 aactgctttc ctttctgata caaaccggcc cactgatggt atacatattg acttgggctc
    35281 caccggaaat atcccgggat ctccacacca aactgaaatc catgggtatg tcccgaacag
    35341 gttagatgaa tgtcggggta cctggaattc acttcgtggt cccaatggct tgggtcgtgc
    35401 gacaacaaaa attgaaggct gtggccaggg accccttcat aagctttggc caggttgccg
    35461 tatttggaga aacgacggga tgcggaaaaa ttttccacgc cgataatact gatagggtgc
    35521 cctttaattt caatgacctg atgttcattg agcagaagtt tccatccgat tttttcatgg
    35581 ttggatttca ggctttccat gttgtcctgt ttggcctggg catcggtcca ttggacgtaa
    35641 tccccgtaat catggttgcc caggatggaa aagctgccat acctgccttt catcccggca
    35701 aaaatgtcga taaatggttc aacttcatcc gcccggtaat taaccatgtc gccggtaaaa
    35761 aaaagcaagt cgggatccag ttcccggatc atatcgaggc tctttttgat cgggtctttt
    35821 gcggtaaaac tgcccgaatg gatgtccgat atctgaacga tttttaaacc ttccagttca
    35881 ggaggcaggc cggataccgg gatcttatgc cggtggaggc gataccggta ggcattgcgg
    35941 gccattccgt acatgagtgt gacaaagggc agtccggcca ataccgcact gaaagtcgat
    36001 agaaaacggg accgggaggc gttgaactga taatccggct taaaccattg taccaggagg
    36061 cctgctcccc gccggatatc atccatcagc acgatgggaa tgctgatgaa tttggaaaaa
    36121 taactgatga atgcaatggc cctcagataa atcaggacta cgtgagggat tttatccgtt
    36181 tgtccggtga aataaaacac gaaaagggtg acggtgacca gtgtgccgaa ccagaatatc
    36241 gtataagccg agccccgcca tacaggcgac caaccctggc tgagggttct gagccattga
    36301 aaactataca ggtcaatacc ggccaggata cataaaaaaa tcaggatacg aagggacatc
    36361 atatgtacgc gcaaagatac tggaatgccg tcagccggtt tgcaaaagtg gagattcaga
    36421 ccccttacac gtccaggccg tcaagtgttg atttgatctg ccgtgccaac gaacccaggg
    36481 ggccctgaaa gtaatatatg tgtgaattta atatgtttgg tccttaacta aatgtatcca
    36541 tagcatgata ttattttggg gcggatatgt tattctaagc caggagtcca gcttctatgc
    36601 accggatgtg aattttacga tttaaaaaac caagcactaa ttatgaaaac cgtacaccag
    36661 gctgtattgt attgcctttt tattgccatt gcccattctg cgcaggccca atgtattgaa
    36721 gggcgcgctt tttttacact ttccggcgat accacggtat atgcctgtcc tgacggcaaa
    36781 ccggacctgg tcggcttcag accgtttaca ggcgggaccc ctttcattta tgtcatcacg
    36841 gatcaaaacg ataccatcat cgggactacc ctcacgggag tggtggattt tgaaaatttg
    36901 ccgggatccg aatatcatgt atacggttat tcttttaaag gggcgctggg ctgggtcctt
    36961 ggcaaacata tcctggacgc ccggatcacc gattattgtt acgaacgttc caccaactac
    37021 gtgctggtga tcaaggaata tccggtagcc ccggtcatca accagcctgg aacaggtcct
    37081 ctttttgtat gcgccccgga ccttaaagcc gattccgtga aaaccgaggc ggtttttggc
    37141 agcaggagca aaataaaata cctggtggct gatgaaaaag ggatcattgt gaaaacctcc
    37201 gacaggcccg aactggacgc ggaccaactg gaatgtgact cctgcaaaat ttatgtcctc
    37261 gcgtataccg ggcggtttct gggcttggaa ggaaattcca tcgacgccgc tttgtccacc
    37321 ggttgttatg agttgagttc agattttcag ttgctggtca gggacctgcc caggggaggg
    37381 aatatcctgt atgccagcca ggactcccag ggctttatct gtgccgccag cacaaaggca
    37441 cagccccttt ccctggacct gtccaacaat tcagccggtt atgaacatta catctttaca
    37501 gataccctta atcaggtgat tgccctgagt gattcggcgt ccattccggg agatgcactg
    37561 attgcgggca tttgcagggt ttggggtttg acctttacag gccaattgaa tgatggatat
    37621 tccgggaagg atattcacga ttttatctgg acggacgatt gttacgccgt caccgaaaat
    37681 tttcttcaga tcaccaaagc cttgccggat ggcggacaaa tcgcgtttaa atccgatgcc
    37741 ggtcttcaga ttctatgcaa ggacggggtt cctgacagcc ttagcctcga agtgaccggt
    37801 gctttagggg cacatggagc ctgggtaatt gtggatactg caggccgggt ctacgactgg
    37861 accagctcat cctcatacga ttttaataaa ctccccgcag gcgaatggaa aatttatttc
    37921 ctttcctata ccggcaacct catcctcgcc atcggagatt ctctgcacgg cgcaccatcc
    37981 gacgactgtt atgattattc ggattcctct ctcaccgtgg ttaccgatgt gcctttaccc
    38041 ggtttgctga ctttttcgga ttcaaccacg acaaaatttt tctgtgcttc ctcttccggc
    38101 aatctagtgg cttttctgaa ttttaccagc cgttccagct taaactcggc cctgctgctt
    38161 acggatgcaa acgataaact ggttcgaatc gttgatagcc tcgatctaaa cctggattct
    38221 ctcgcgaacg ggaattaccg catttatagc ttgtcttatt cgggtgacct gctggcagaa
    38281 accggtcaag tccttgatcc catgaacttg tcttccggtt gcagtcaatt gtcagatcaa
    38341 tacctcggcc tcatgattga agaagtggaa gccgccgaca tcagtctgga aaatgggcag
    38401 gacagcctga cctggtgcgc cggggatacc ctgtccacca ctgtattggg cacaaatgca
    38461 atgccgggga cccctcatgt gttttttgtc acagacctcc gggattccgt attgcatctg
    38521 ctggattccg atacccttcg cgccgcaaac ctgaattcgg gggcctcccg gatccgaggg
    38581 ctgtcctatt cgggacaatt gttgttgcaa ccgggccagt tgctttccca gcaaacgtta
    38641 gcctcagggt gcgccgccct gagtaccaac gtcatcaagg tgcttaagga aaatccgcag
    38701 gggggcaggg tttccctggt ttccggggac accagctata ccatatgttt taaagacggc
    38761 tggcccgacc ggccggcttt gaagagtaca tccacttcct tgatcgaata tcagtatatc
    38821 ctgaccaatg aacagaacca gattattgac ctgaacgcct cggaacttga tctggagagc
    38881 cttaacagcg gccggtacag aatctacggg gtgagttacc tgggttctgt tttggccaag
    38941 gcgggggatg atatgaccgc ggtgcccttt gcttcgggct gttttcagta tagtgaaaat
    39001 gctatccaaa tcagttctga tgaattgtcc gcagggaccc tgcgtacccc gaccggtgaa
    39061 aatgtgctgt attactgtac ggatgatagc caacccgata cggttttgct taccagcaac
    39121 ggagcgaccc caggcgcaaa atatgcctac ctgggaatcc aatccgatac cgtgaagtac
    39181 atttcaatgg atggcagaat catcgatgtc catttgccag ccggaaccac cagggtattc
    39241 ggagtggctt accggggatc cctcctggtc aaagccgggg accgggtttc gtccgcccgg
    39301 cttaccgaca gttgtttttc ggttacttcg aatgaagtga tcatttataa atatgcacct
    39361 gaagcgggaa ccctaactac gagtggtggt cagactgaat tttttctctg tccccaggat
    39421 gggcagccgg attttatcac cgtagaggag cagggtgcgg tacccttgcc ttatgcctat
    39481 gtaatcaccg acgaggcgga cagcattttg gcctggagtt ccaattcttc gtttgacctg
    39541 gataccttca gtcttggact gtgcaggatc tatgggattt cctactctgg aaaccttcaa
    39601 ttgtttggaa agaagttgga tggaccagac ctgagctccg gatgttttga tgtttccgga
    39661 caattcatca ccgtctataa gtccaccgcg gatgccggcc ggatcagttt ggcaggtgga
    39721 gacaccgtgg tgaccatctg cgtggaagac ctggccccgg ataccttgtt tttcagtcat
    39781 accggagtcc agggactaaa gtacgcttac ctggtcaccg atctgaacaa ccggctgcag
    39841 gccatcgtag aaaaagatgg tcccgaattt gattttaatg gcgccgatcc gggtaccagc
    39901 aggatttacg gggttgctta tggagggatc ttatcggtat tcaggaatga tgatgtcacg
    39961 gctaccgcat tgtccaccgg ctgttacgat ttgagcgata attttatact tctgaataaa
    40021 gtaaattcgg gtccgggctg caagacttcc ggtattcagt ccgggaacca ggtattttta
    40081 tcaatatttc caaatccggc tctccaaagg gtcactctaa gcctgaaggg agattacctg
    40141 aagtcaggaa aacccgagct tcacctgatt tcggtgaccg ggcagttggg taaaaaaatt
    40201 aatttatctt cagaagatat tgataatgag aaagttgtat ttgacgtcac ttccttaaca
    40261 ccgggattgt atttcattat tttcaaaaat ggttatatat ttgatagagt caaaatgact
    40321 gtaattcgtt aagaaaagat attatcattt tatttgcctt ttgatgtttc gaaccccggg
    40381 gatattattc ccggggtttt ttttatggat atggtacgat ttatgctatg gtcatacgtt
    40441 aaggattaag tactaattaa ttgattcaaa acaaattgaa tcaaagatga aaaaatcagc
    40501 accaatcatt ttccccggaa aattcagttt aggcttgctt gccctgacat tcctcctgca
    40561 agggtaccgt gcctgggctc aatgtttgcc gcacacgatt tcggtttcga cccttgagca
    40621 agtgctctat gcctgtcccg gcgatggaaa accggatgag tttgtttttt taaaatcaga
    40681 tacctcccaa aacaaatatg cctttatcct cgcagacggt gcggacaaca tcatccgggt
    40741 tcaaacctcc ccggttttta attttgatgg tgcgctcc
//
LOCUS       DAOQXT010000034        40725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1099_length_40725_cov_8.862626, whole genome shotgun sequence.
ACCESSION   DAOQXT010000034 DAOQXT010000000
VERSION     DAOQXT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 40725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40725
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_40725_cov_8.862626"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1250)
                     /locus_tag="PKM27_07915"
     CDS             complement(51..1250)
                     /locus_tag="PKM27_07915"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07224.1"
                     /translation="MHSIALITTWLLFCTTLASQTIPKLYIQIVSHNEPNDNFNQPLR
                     YAKAKKDLVQLANIVDSKNAKWNLQTPDGLVFGARQDEISTGGNIFEQLANAPYRDNI
                     EIDPRSKNNPGRNIADQWYLLDSLGARPSKTVGGFIYYVCPPNSSSLIDWWQYTDTIT
                     GLYYGNKIKFQLLSGAGSLAPHCNDLNDFGIFKPDTTTRFYTHNPARNLWCIGTGCAP
                     LLDSAADEQAIIDLIQSQVDLIQKRIWPSDKFYVTRIMTNQREYGPMFFQKMAKVIDS
                     LNLIPSTQLKWATLSEAFADFEFWQESTNREYSQWLCNQTSTRLLQRSHTVSYRLYPN
                     PFRGVFNIEFDDSMEHQLAVFDLTGRKVSYKSKILYRDRINLSGSPLGTYFIQVDDHR
                     LEKVVLL"
     gene            complement(1437..2114)
                     /locus_tag="PKM27_07920"
     CDS             complement(1437..2114)
                     /locus_tag="PKM27_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HNR07225.1"
                     /translation="MKNRMLAFAPAILLMAATLFSSCSKDEDLAQANVLVIHASPDAP
                     GVDLLVDDVKQNTSALTYPQNTGYLKVNAGSRNLKVNVAGTTTTVINANVTLEQDKSY
                     SVFVIDSVKKISAVVVADDLSTPAAGKAHVRFIHLSPNAPAVDIAVDGGAVVFPNTSF
                     KSGTAFTPLDAGTYDLEVRVAGTQTVALDLDPLTLEAGKIYTVFAKGFLTADGVQALG
                     AEIIVNK"
     gene            complement(2417..4627)
                     /locus_tag="PKM27_07925"
     CDS             complement(2417..4627)
                     /locus_tag="PKM27_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNR07226.1"
                     /translation="MTRFHQTIMPSVKLKSFLSLVTFLFLSACQPDINNDRTWSVYKA
                     DAESSSYSPLKQINKDNVHLLQPAWKFIPNDSRNNRIGSSECNPIVIDGILYTTSARH
                     RLYALNAGTGEIIWSHDPFEGGEGGGINRGVTYWEDGEDKRILYTAGDRLFALHALTG
                     KPVIPFGDSGKVSMNIGLRGDPATISVIPTSPGIVYRDLLIIGAEVSELYGAEPGYIR
                     AYNIRSGKLEWTFHTIPHPGEPGYETWPRDAWKYAGGANDWAGMSLDKKRGMVFLALG
                     SPTYDFYGADRKGQNLYGNSVVALNAQTGAYIWHFQTVHHDLWDYDLPAPPNLVTLEK
                     DGKKVDAVAQTTKSGFLFVFDRETGVPLFPVEERPVPPSLVPGEEAWPTQPFPLKPKP
                     YSRQSMTDADLMYHSPSSYDSLLKRFRSFRYEGLFTPPDTRGTLMLPGTRGGSNWGGG
                     AFDPATGILYVKSSDSPEIELLQKIETEKTAGSKSGYNQGMSLYTKHCAACHGRDKNG
                     DEPNFPSLVGLSARMDRETALEKIMKGSGRMPGFAAVMQGNEEAIISYLYDKADRISR
                     KESYLLEIRENKLAAKDSSSESEPSDTAALYLNITAYGHFRDPEGRPAIKPPWGELNA
                     IDLNTGEYAWKIPVGNHPEYQKPGEPITGSEGYGGPIVTAGGLVFLGSTHDKKFRAFD
                     KDNGKLLWEIDLPGVANATPCTYWSGGKQYVVTSVAGDKENPSGYLLAFALPQQ"
     gene            4716..5936
                     /locus_tag="PKM27_07930"
     CDS             4716..5936
                     /locus_tag="PKM27_07930"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNR07227.1"
                     /translation="MYNPFGGQLPPRIRCNKFVNWLIVIQCLYPFHGIAQIDPIPVFV
                     SGQDGHRSYRIPAILSLPDGQLLAFCEGRVSGSGDFGDINIVMKRSPDQGKTWSAMQT
                     VVNYDSLQAGNPAPVVDLLDPVNPGGRLFLFYNTGNNHENEIRKGNGLREVWYITSTD
                     GGQTWSQPINITAQVHRPIKSEVNSEYRFKEDWRSYANTPGHAIQIGSGKYRGRMYIA
                     ANHSQGDPKPHFEDYFAHGYYSDDHGKSFVLSESIPIPGSNEATAAELSGDGLLMNIR
                     NQKGDVKARIVARSRDGGSTWDTVYFDANLPDPVNEGALLNLGKTGKRHRLAFSNAAD
                     TLHRNRLTLRISRDEGKSWYKNILVDAVSGEQARDHTAYSDLVKLDRKTVGILYERRG
                     YSQIVFRAVNWKKE"
     gene            6223..7866
                     /locus_tag="PKM27_07935"
     CDS             6223..7866
                     /locus_tag="PKM27_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR07228.1"
                     /translation="MKTMLILVVLFCLADRSKAPSPPVRKADVIIYGGTSAAVIAAVQ
                     VKKMGKSVIVVSPDHHLGGLSSSGLGFTDTGNKEVIGGLSREFYQRLYRYYQDSSTWK
                     WQKKEEYGNKGQGTPAIDGANRTMWIFEPHAAEKVFEDFVKEYKLEIYRNEWLDRTSK
                     GVIKKSGGIRSFRTLSGTRYRGNMFIDATYEGDLLAAAGISYHVGREANTVYGETWNG
                     VQAGVFQHGHHFKSRISPYKIANDSLSGLLPEISYEPVAPNGSGDRKIQAYCFRMCLS
                     NHPDNRLPFPKPEGYDSSRYELLARVFASGWRETFDKFDAIPNRKTDTNNHGPFSTDY
                     IGKNYNYPEASYEHRERIIKEHELYQKGLMYFLQNDHRVPEEVREKMKEWGLPKDEFT
                     DNGHWPHQLYIREARRMIGEYVMKEADALGKTQVPEPIGMGSYALDAHNAQRYVQSNG
                     FVQNEGDIGVHPDKPYSIAYQSILPKATECKNLLVPVCLSSSHIAYGSIRMEPVFMIL
                     GQSAATAAVLSIQHKVSPQKLNYETLKTVLLKDKQRLEN"
     gene            complement(7930..9777)
                     /locus_tag="PKM27_07940"
     CDS             complement(7930..9777)
                     /locus_tag="PKM27_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07229.1"
                     /translation="MKKNKFLLYLFFAGPFWFSCKPEADTDQDVHHYALSRKNDLKLS
                     VYFTAQAVDQLWTGETGKREALSMLRANGISSVYLEVYRSGLVVSNARLDSVIRFFKT
                     NGFEVAGGIATVPGGNFGLKQDGKFQWFNWQNEKTRKDLSSLMEKTAPLFDAFIVDDF
                     VCTADTSVESQTAKGDRSWSAYRRDLLTDFSEKYLIAPAKKANPDIRVIIKYPQWYDR
                     YHLFGYDVTRQTALYDQVWIGTESRGQYTPRFGYVQPYEGFVNYRWMSSVSGDKMGGA
                     WFDHIDCDQNDFIEQAYQAVLAGAKELILFNSYNFMEGHPGQHFLRSEFNHLADLAAL
                     LSKTPVDGISTYKPPNSDPGGDMYIYDFIGMFGVPLIPYAHYPSHAEIIFLPAQAAED
                     SAIFEKIKKSMASGSRLILTTGFLNRLSQGQELAEMAGIQWPIDTPVIPTPEILVNGK
                     PDTLDLALSLEGNIKPKDATVELTAIFNKQEIPFLCRNAKGNIYVLNVHTFSDEDFKS
                     TGEELLCPKPLGLMEVPLAWANVIRKVFNGNEIIPMDGPARVSFQPFGKGEFLVQNYN
                     PKLAQVKFNSLTGSYVNVLTGDSLSTNIEGLILNMAPRSRYWIREAGGE"
     gene            9915..11681
                     /locus_tag="PKM27_07945"
     CDS             9915..11681
                     /locus_tag="PKM27_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNR07230.1"
                     /translation="MKSGILVLVLTLAAMRPVLAQEGKIKIWNPATDSIQCLEGQAWA
                     GSGKNVFARLPANLENKVRKEVWNLSQHTAGLSLRFRTNASELTVRYTVKGGHQMPHM
                     PATGVSGMDLYSKSIDGQWLWCAGRYTFGDTLSYRFVNLPSKDQHVSNWEYTLYLPLY
                     NSIKWMEISMPAECEWRPLPVRNEKPVIVYGTSIAQGGCASRPGLAWTNILARKLNSP
                     VVNLGFSGNGRLETEILELMGKTDAKAFVLDCLPNLMPPAFTGEEVKNRIQQAIGLLR
                     KARPEVPILLTDHDGYTDEAMNEARRKDYTGVNQALHEILYSIRKEGIGRLYHLPKAE
                     INQDIETMVDGVHPNDIGMMRYAEAYERILRKILDMEDGGTVTTTAVTQRRDARIYDW
                     ETRHEEILDYNKNHQPEIVFLGNSITHFWGGLPPNPVNNGPEAWIKHFGSRRIVNMGF
                     GWDRVENVLWRVHHGTLDGFKAKQIVLMIGTNNLHLNTDEEIVEGLRYLIQAIQSKQP
                     QSTILLMGILPRRNTEERVVQINKKIANLASGKNIQYADAGPLFVRADQKIDEALFTD
                     GLHPNEMGYDRLGGFIIRWMDQ"
     gene            12075..12698
                     /locus_tag="PKM27_07950"
     CDS             12075..12698
                     /locus_tag="PKM27_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNR07231.1"
                     /translation="MDLLGTFAFAISGIRLASGKNMDWFGAYVIGLVTAIGGGTTRDV
                     LLDLTAFWILDPSYLLTTAFALLVTLLFREKIMLWGKPLFLFDTIGLGLFTIVGIGKS
                     LEAGLPAWACIMMGTITGSLGGVIRDVLINEVPLLFRRDIYALACIAGGIVFFICQKT
                     GLGSFSELVGAASVILIRMLAVKFHIHLPTLIPEHIRNNSPGDSSEQ"
     gene            complement(12755..16123)
                     /locus_tag="PKM27_07955"
     CDS             complement(12755..16123)
                     /locus_tag="PKM27_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07232.1"
                     /translation="MIRNHSYGNLRILISSLIALYFVVSPFVPSEKSLSAHRTGPVAS
                     PSWAKPEFHKDREEYEWMISHDPATGRVPFNARNRDRLAVQAIPRVKRTLRSQTYISA
                     GPVNIAGRVRSAAYDIGYNGTSNRLILAGGVSGGVFRSTDGGMNWTWIPTPNLNSVTA
                     LAQDPRVGINPVTGKPYRDTWYAGTGELPGGSATVWGHTHHGFGVMVSDDKGLTWMPV
                     KITQAGKEFLFDNRYDYIGKLVVNPQDGFIWVSKWGGFSQLNRKSLAEFSIQQAFEIG
                     NGDVGGWDANDIIISKSGSKIYLAFNGQSTAVPVKFPNTTYQGIWEGENAVPIQWKKI
                     VDSVLFNIWPAPKKYYRISIALAPSNESILYALSVNQPNGPNLIFPTGDLFKIELDMS
                     GNKVTELSQNLPSNAANENFGGLNTSGGYNLALAVKPDDPDFVLIGGTNAYRSASGFR
                     SKTATRLLGGFNYRNAPAGSTQGYDGDRSHPDIHGFIFRPGSSSEMIHFHDGGLSLTT
                     QVSADTVRHQYINTGLQNTQYYAIAIDPNPGSLSFIGGTQDNGTLFYNGLSPNPKNQI
                     RIYTGDGMTHGISKITNNTKWLLISGQGGYCLRQMVNPANNAFLSRSGLLQPKGSTSA
                     FRTLIYLNPDNTEDLYFTGTNVLYRTTQASMVTDSTWQVLPGTKIGDNEQILCMASTR
                     GAYDNLHNLFYGSSAGKLFRLRNPRMAPPNQIPDNITPKENGVLATGSILDIAVNPRN
                     DDTLMVVYSNYNVKSIWWTGNANAAIPNWINIEGNLAEPSIRSCEIIVKKSGVEYFVG
                     TTVGLYSTTMIDKSGTQWFKENENSPMEYAVISSMDHRWTDNTLVIGTHGNGTFYSQI
                     GDAVAVTGIIAPLVITRHPESKSVCDQKPIVLEVTAQGGKGTGFQWYKNNMLIPNATE
                     ASLSITPMESAGYYCEVKDLSSTLKSNTAQITVSKSASAEISGTASINPGDSVPITIT
                     LTGTPPWKVSIGKDSTITLSSSPYTFFVKPVQTTEYTISSLSNECGIGFYFGVAKITV
                     KNTTPLKELALNKFELFPVPASSAITFRFEKLASEKMVYRVVDSRGKIHYLQPVPELV
                     DQGHTLNLETLPSGAYYLSILSEDKIRSVKFIKI"
     gene            complement(16154..16552)
                     /locus_tag="PKM27_07960"
     CDS             complement(16154..16552)
                     /locus_tag="PKM27_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07233.1"
                     /translation="MAFRQKVIPGAAPDPSSPAVSMYRYYLFLLPQNNSSQIRVVQIC
                     VDQMIYGAVLERLQEPYITEPIYGPEGYRRDTLLPKDKLPAFLIHLKKPETAAGINQA
                     VCEKNQLVLELNIGKVIINHWTDLPDQIMP"
     gene            16909..17361
                     /locus_tag="PKM27_07965"
     CDS             16909..17361
                     /locus_tag="PKM27_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07234.1"
                     /translation="MFKQVLALTLALAFLIACKSEHPDLKTAYELFKEGFEQHSYIIT
                     HSAELKNEAKMVDSRIRNEDKESVFAISEVYNHLDEVDGGLKNLVGRIQAVPGHEKES
                     FGAPVLDKKMEPAQILMIQKNYRDSVLLIKNELDAAATLLNALKTKLN"
     gene            17436..18932
                     /locus_tag="PKM27_07970"
     CDS             17436..18932
                     /locus_tag="PKM27_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07235.1"
                     /translation="MDLYIPEVFINARQEVYLILKNQGNTFIPHRNLELEVCWDDSSM
                     VRFNLDSMDPHFRYPGDSSIFQLPFKVQRSRHLVKARVDRLNQLAESDEVHNVYSRTL
                     KTDFPEAPVPLTYFPGSKRFEPAKYLDLILQAPFASGILWYEDHVVYPVAYWRKAWKD
                     QLISKLGAIYSGTPVPLPDTLPWSLSRDVAFGVYLNYIAHSLWLEMEKEVPWSLRGMN
                     PDDLSGLWDARQFFEWDSLSREYHPGYQISGAVSPQHPLVSYRFAAFLNGLPEDIPGS
                     IRRFLDWGRAYLVHAEGKGLPVFQTAKDIYYPESGQLHAVNSCWASGGLMADYCRAFN
                     IPVRRHNIELSNGIHNQLEFPTEAWYLVHADDMYDPLFYPLPGSISSTRMLFNKSEFN
                     QMLRGRPHCLEDSCHSKGTQLSFERRRNLLSKALDLNSGYLMLQYDQGRNKFREFIRG
                     DRFEKFLVPLFNEEEERAMMEKIKAAKPDYHFLSTLYERFEACKNNRR"
     gene            19011..20333
                     /locus_tag="PKM27_07975"
     CDS             19011..20333
                     /locus_tag="PKM27_07975"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNR07236.1"
                     /translation="MKVMKFGGTSVGKPERMMEVARLITADRESKIVVLSALSGTTNK
                     LVEISTCIAQRDKARAKTEIELMEAHYQAFVRDLLHTEPAIAKATEVLREHFEFLNIA
                     TKISYSEALTKDILAQGELLSTKLFSCYLEEQKIGHHLLSAFDFMSIDENDEPHISVI
                     RSKLVPILADKSDSQIYLTQGYICLNARGEVDNLKRGGSDYSASLIAAAIQASVCEIW
                     TDIDGMHNNDPRIVKTTRPVEQLSFDEAAELAYFGAKILHPASIWPAQQEHIPVKLLN
                     TLAPQAKGTLIAKSASGQGVKAVAAKDGIVAINIKSSRMLLAYGFLRSVFSIFEKYRT
                     SIDMITTSEVAVSMTIDQTGSLNQITAELEKLGTVSVITNQTIVSIVGHNLIQSPNLL
                     RDLFAGIAHIPVSMVSYGGSPHNISLLVPADQKHETLQSLNRHLFGLD"
     gene            20415..20591
                     /locus_tag="PKM27_07980"
     CDS             20415..20591
                     /locus_tag="PKM27_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07237.1"
                     /translation="MKKIQILTVLMLVLTVFACKNTGSEAAAPAMDNTSVAAPEAAPA
                     VDTMAAAQDTSMQK"
     gene            20693..20968
                     /locus_tag="PKM27_07985"
     CDS             20693..20968
                     /locus_tag="PKM27_07985"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNR07238.1"
                     /translation="MKQILLFAFFALVFAVACKNTGTEAAAPAADTTMMTTTETMTAA
                     PDTMAMAAFACPMHPEVTGKEGDKCSKCGMDLVAVKKEEPGHEGHDH"
     gene            21771..23507
                     /locus_tag="PKM27_07990"
     CDS             21771..23507
                     /locus_tag="PKM27_07990"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNR07239.1"
                     /translation="MANLIFISYRRSDIPIEQVNLIHEGLENVFGEGSVFLDTADIHG
                     GAKWKKILNEAGTHAKVCLVLIGPRWMEKDDQGRIRIQQEGDWVRQEIEYAMEKNLVI
                     LPVLVNGGVLPQKEDLPDSLKSFNDSQGIVVRTAEWSTYKEKLIRDLKKNIKGTSKLK
                     SLLWILLPAAAVVGGSFLLPNFIKRDNTQAPPCTSFATNAELKTLLFPVYARDRHSSQ
                     ETIQLIDESFSTKCTKYGISFENQLANTRFDTIIVRSSKIQIAKTCGADLYFSGRWVG
                     EPGQQGFEADFQLVRDTINAFVAAASDLSIKLTRFSVNELLGDEAVEEWYEKVIQFLV
                     GLVAYQEGDFKQSVLALKEAVSDTLRQDSLKLLAYSIITDAYYQLKMPDSCLEYQQKL
                     TRWSPTEQVVLKTAILAEQYNRPEVAIVSYTTLIDSGKYDKNVLLEKRADQYAEIKEY
                     SKAKQDYQRVKTNENNKERVNAKIERTNTQIERNRAAVTSVNNLQATETVKIRAADLY
                     LQNGQANKAAELLKSIPKNSEYFKQAEPLLQEAQLKINPSAVTVTEAAVKANQRLKTE
                     AGLRNLAVKKND"
     gene            complement(23604..24995)
                     /locus_tag="PKM27_07995"
     CDS             complement(23604..24995)
                     /locus_tag="PKM27_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07240.1"
                     /translation="MKMKQRFAFLLFNLSLWAGPPLLSQTVVTLTECNHAGWVKQFLD
                     PNGKINFINAPQNSFMGKGSLEMYSPQGYVRFRNTLHHHRLLSEFTELSFSAIIANRS
                     QMVDAPYIVLQIDTSGDNALDANLVFNPVYQTGSYAQNAQVPDQGLILDNVWQTWDAA
                     NGAWWAGIPPEDNGPLFTLADFVQKYPTARIVNQGAMGSGGIRLTVGGPVGIFGTDFR
                     AYIDNFRIGINGATTIYDFEFFIAADAGPDQTVVYGSECTALTAQAAGGAEPYQFAWS
                     GGASPDSMVNLICPQTTTTYTLTVTDSLGCTGSDQVTVFVLCGPHSNKVRLCHNGHII
                     CAAAASLKAHLAHNDDLNDCSTSSLIVPDRVPGKLQKVKGETVSVYPNPFSEYVRFAY
                     EVPVEASVLLSIQDFTGRTVAVYDAGIKSPGVYTTDLSAGNFSNGIYYYTLVFNAPGQ
                     TPEHATGKLMVIH"
     gene            25447..25716
                     /locus_tag="PKM27_08000"
     CDS             25447..25716
                     /locus_tag="PKM27_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07241.1"
                     /translation="MVNNLQATETVKIRAADLYLQNGQANKAAELLKSIPKTSEYFKQ
                     AEPLLQEAQLKLNPASVTVTESAVKVNPRLRTEANLKNQVIRKVN"
     gene            complement(25741..26865)
                     /locus_tag="PKM27_08005"
     CDS             complement(25741..26865)
                     /locus_tag="PKM27_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07242.1"
                     /translation="METREVLTRQDWNLFFNLPFSLYKNDPCWVSPLRMEQRRTLDRK
                     NNPYFNHCIYKAWLILDKKEALGRILAYIDSGYNELHREQAGFLGFFESREDSAVSSA
                     LFDKALEWLRQKGLNRVYGPMNFSIANECGILLTGFDSPPFMQMNHTPPYYRHLFEQA
                     GFSKAHDLYAYLMTEELVKQNGALLQRMQGISEKTMKKEGVRFRTVNLKNYHVELANI
                     NTLFNDFMKETWGFVPSSIEEMYYVGETLKQIVDPEMIFFAEFKDKVVGCSLTIPDIN
                     TILIHMKGRLDLPGILKWFYYKRKIKSFRLLMLGVAEEYRRKGLDILFYYYTIQKGLE
                     RGYTKSELSWISEDNAVLISIVEKIGAIRYKTYWIYEKPV"
     gene            complement(26867..27706)
                     /locus_tag="PKM27_08010"
     CDS             complement(26867..27706)
                     /locus_tag="PKM27_08010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR07243.1"
                     /translation="MIDHEYEEKVVVITGGSSGIGLAVAKEFALRKAKTVLISRNEEK
                     LIQAKKELEKISGLPSGIFYYPADISDREQINMVILKIVDRFNGIDVLINSAGIITCG
                     RFADQPIQDLEACLFTNYLGTVYATKAAWPWLKKNKGLMAFVSSVAGYTGVIGYSGYA
                     PPKFAMTGLVECLRLEAKDDGIRICIIYPPDTDTPLLEYERKHSLPESIALNKNIKAI
                     TAERVAKIFMDGLLKNKFEIYCDFNSRFVRWMKNNFPGLFNRLTDSIILRERNRLSRQ
                     TNP"
     gene            complement(27703..28890)
                     /locus_tag="PKM27_08015"
     CDS             complement(27703..28890)
                     /locus_tag="PKM27_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNR07244.1"
                     /translation="MTTALAHRWFELLKLGADEDIYTFQEVIKGKSGPKVNIRDKPVY
                     MMSSYDYLGLIGHPEIESAAIDAIHEYGTGTGGVRLLTGTNVLHHHLEKSLSRFMGTE
                     SSMVLSSGYMANLAVIAGLFSKNDLVIADERIHRSIVDALKVAGIQPEPFRHNDPLSL
                     KSVLEKNDRVKRKLILVEGVYSMDGDICPLPEILALKEAYRAFLMVDEAHSLGVLGKT
                     GRGVAEHFGIDPGRIDLFTGSLSKAIPANGGFVSAKEEVILYLKHGGAPYMFSAALSP
                     ANSAAALKSLEIIDKETWRLNKLWQNTSRMMTGLRKGGILTGSTQSPIIPVSCGKNED
                     AAKLSKGLLDQGFLATAVIYPAVPLNSAILRLCGTAAQSADRIDQFAEALEELYTTVI
                     KQS"
     gene            complement(28859..28987)
                     /locus_tag="PKM27_08020"
     CDS             complement(28859..28987)
                     /locus_tag="PKM27_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07245.1"
                     /translation="MKTLNLEGKQNQYLVKEFFNRKPNFRSRDHLLLDHRIGASLV"
     gene            29117..29650
                     /gene="cysC"
                     /locus_tag="PKM27_08025"
     CDS             29117..29650
                     /gene="cysC"
                     /locus_tag="PKM27_08025"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNR07246.1"
                     /translation="MLLIQLTGLSGSGKSTIADGTRTRLLEKGLPVEVIDGDIYRNKL
                     YPELCFSREERCENIRRLAWLANRFVSHGVITLLAAINPFEEVRREIKANGPHVKTVW
                     IRCDLETLMKRDTKQLYRRAFLPEGHPDKILNLTGVNDPFEIPEKADLIVDTTFEVEE
                     ISIGKLYHFILNTLETA"
     gene            29914..30702
                     /locus_tag="PKM27_08030"
     CDS             29914..30702
                     /locus_tag="PKM27_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07247.1"
                     /translation="MKVILCFCLLILTNCGTVSKLPKTQGDPLNVNSYGYHPLDPLPV
                     ELEADTANPLTNSRLLKAMPDETIRLAIGQVISTGGLKFTTASAGYEGNSYVVVLDYI
                     KFNTNSFNVKVDRSNGRATKANVLEQSSQEGDAIIPLYIGVGLRLTANITINKGQVDL
                     GNLFAIGAQAASQRISGTLTVQTLGISGENISAVIPMPSEINTTTIQNAILALGMIKA
                     KIYEDNTTITPRVVGVYNNIGGSTEVINGFISSILQKPKILAVK"
     gene            31120..36444
                     /locus_tag="PKM27_08035"
     CDS             31120..36444
                     /locus_tag="PKM27_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNR07248.1"
                     /translation="MKTIKILGRPVLKEEGGIEALQSQFNKVEKSFRLESLRGETKVM
                     DTGAKAGDIIALEFEDGGEWIGSADDIEEIFGIPQPAKRGAGADIFPSRLAVNSGERG
                     LQDIGIKFLHLIRGKGTKSISQAAAKKLGQTADEKMMPKPGLFMVNGQGILTPVKTGL
                     KPDKYLLFIHGTLSSFHGSFGDLFDKESENDLGQTIIRQYQNKVLALQHFTLSQSPFD
                     NALEVLKLLPKGSSLDILSHSRGGLVADILACCDKRNVLKGYSAENITLARDQDESLA
                     KTLEQINALATRKEIIVEKVIRIACPANGTLLLSNRLDHFLNGFLRAIGLLAGGRSNP
                     VYPFIRGLITDVIRARLTPGILPGLWSMVPESTPQRLINRPEVMLSNHLLVIEGDAEK
                     GKNFWQSIQVVLTNLYYREDNDFVVHTGSMRYGALRQSGLYFFLSKDEDTSHFNYFKN
                     ENTRLAIQDALNTPPGQPVRRFEYRAASERDRGVLVDLFVSLKAISADRISGNKPVVI
                     LLPGIMGTHLSVNGQIIWADLGEIGKGRIVTDLHAGNYDVGTTHIIGDFYQKLVGYLG
                     KDYDVYTMGYDWRQTLAQAVGKLNTLLEDIRQRAPLQTISIVAHSMGGLVVRDMMRLH
                     PDPWSAYIERPGSRVILLGTPWLGSHLIMEVFTGHQSRVRQLSFLDTRHRRDELIRVF
                     NQYPGVYELLPINEEPFEKDSFWNSINAELESDKILLPPLLNEFNRYKNQVKAFSKNP
                     DLKNIYYIAGKSDHTTCAYRIRKNFFGKRLQYLGTPEGDGSVTWMLGIPKTLPVERLY
                     YAPDTGHGDLANDESLFPGIREILTNGATRQISQQRPVLPAIRSVMNKEGIQIIEPMP
                     DTLVSNRADEAYSILLDRPRATSRPARSNTRILEVSVVHGDLRIASHPVMAGHFVRDG
                     ITNAEAAIDYYFDRKLSERHRLSGYPEQVGESLVLFDPVKYPKGTVILGLGESTALTP
                     FHLRKTVEAGIISYAMHFRDNVSTAIDGHDTAVSCLCIGTGYGRLPMEEALHAIVTGV
                     IRANQQTETLNGLKAIKKIEFIELYEHIAQNAYYQLSQLENNQTSGPAFKLKKGIEKK
                     AGARRKFQFSNENSWWHHFTTRLMGKDETSKIPRLQFTSASGIARVEEENTFTSGKII
                     QALLDQMAVDQGLWNKEYSKTLFEILVPNPFKEIIRHQNNILWKMDLDTASFPWELFH
                     DYETDSMPAFARAGMIRQLYTPDTEASPDIVRCQKALVIADPQYSADGPGQLPYARAE
                     GIQVSKALKEGGFETESLINRSGLDILNNLYNKEYKILHIAGHGTISDNPHETGIVLE
                     NGMYITPDMLKNLSRLPEFVFINCCYSGTVEPGKEKQYQQRYRFAANVGTQLIRMGVN
                     AVVVAGWPVNDAAAGLFAEKLYRSMLAGKTFGDAVRLSREACWTQFGDSNNTWGAYQC
                     YGDQWYRLTDQNAGPSTEVDYFTEEQILVDLYNFKTKLEGKKIKDRLALQEHLDKIVQ
                     RALKAGLYQALVIEKEADILSELDLVSPALERYKSLRSINQADFSVKALEQYCNLRMK
                     SALGDKRITASQIRQLITDFHALGMLGLTPERLCLMGSAYQNLAIIAHRNPSKTMPSV
                     TVSLKEAEKQYKKAFVLSDPAKVQDLIYPLANWLILNAINDGPPTIEIKGKKMNISSF
                     LNQTEIKLKKELGRNLNFWQDISLVNLGQCKLFYTSSLVELDGIKNEIINNYKNNWNL
                     SGTLKHLQTELDQIDFLIFGVERMKKSKRWKERMKKNLAEIRDELKEM"
     gene            complement(36648..37322)
                     /locus_tag="PKM27_08040"
     CDS             complement(36648..37322)
                     /locus_tag="PKM27_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07249.1"
                     /translation="MNNFESSLRKVILAPYITKATALIGVRRRSGSNMFRHQISTLGI
                     LLDYKMTDPILLKASVIHDLFEDGSHLPGVTEGEIRQLDEDGPAVYDLVMEVSIRRLN
                     GEKEPKAEFLQRVMKSGSQRAKILKLADRISNLFALGFVHDTGFIRKYLEETKAFILP
                     YAQAINADMFIELSDLVKSRERMLEILSAEVPGEIKSGAKNSFWASAMHWLNGLRNRV
                     FPEYHS"
     gene            complement(37380..38447)
                     /locus_tag="PKM27_08045"
     CDS             complement(37380..38447)
                     /locus_tag="PKM27_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07250.1"
                     /translation="MKRLFFVLISFYATESFAQRVMAVQTPAFQRDLLWYSCNFTKLD
                     PALRDSIANTVASAAPGTPRERAREVNELINVVNYSSEAFEGISQKLTKPMQKLRNYK
                     FYFSASRDTSYRSDAKDETQYKKYPLKLYVFGSDGHCGFGELLATPFEIVNHRWDKYV
                     MNLRGTKEWSHIVIYAAHNIQDSAARNRSQSYLMISQHGPVTASGSLSGRRIRGFDPG
                     IFKLETYSTLSFGGIEMMYELSPKAISVFDKNSEIIVSDTLRKDLDHNPTLDTILNKF
                     RGIDYLFLIMNEKKYSKKRLNVISYLKTKYPWILDSYKVMKYSTLTSKRKNRALLKRV
                     YDKKNEIGDLLIVERVQEFVK"
     gene            complement(38548..39267)
                     /locus_tag="PKM27_08050"
     CDS             complement(38548..39267)
                     /locus_tag="PKM27_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07251.1"
                     /translation="MGIAKQFSRIIRRELRVQAAWLPVVNVFSLGDYGVIDGGIFVRR
                     GNIKTDFKISFETLESPDAHIDFKSKGTRLIKLDAGVPVQNIPSGSVNAQVKVQFSGA
                     RSFLIQSPLIKVKEIASPNVLAMSLAGQAFWRAEYKVVHQVYHAKKAVVVSTKESNTE
                     LIFSGQAKALNDLDLGHAALSMSFNKSIGLDLNGKEGIIGLGLFRIMDGQMGVVRGKA
                     KSEVKKTRIEMIGGEEMGDDF"
     gene            complement(39510..40283)
                     /locus_tag="PKM27_08055"
     CDS             complement(39510..40283)
                     /locus_tag="PKM27_08055"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNR07252.1"
                     /translation="MSFPGSLLPAYSLSPLQWVLLILAAAIIGMSKAGIGSISIITVS
                     ILAWIFGSRASTGMVLPMLIFADILAVKHYKRHALWEHLVKLLPWIAAGVVFAAVFGH
                     LINDRVFKQLMAAIILVSLAALIYYEKRPLASVPDHWLFAGSMGLLTGFTSMIGNLAG
                     GFSNVYFISMRVLKNSFIGTAAWMFFLLNLFKLPFHIWVWKTVTPSTFKLNLVLVPVI
                     AGGFYLGVFLVRKLNEKGFREVILWLTIGSSLLVLFDLR"
BASE COUNT        10821 a         9537 c         9945 g        10422 t
ORIGIN      
        1 attttaatat gttcctaagt ctcaactcct taggctactc gtgaacttcc ctaaagcagg
       61 accactttct ccagacgatg gtcatccacc tgaataaaat aggttcccag tggtgaaccg
      121 gaaagattaa tgcggtccct atagaggatt ttacttttat aggatacttt tctgccggta
      181 agatcgaata cagcaagctg atgttccatc gaatcatcaa actcaatatt aaacaccccg
      241 cggaaaggat tgggatacaa acggtaagat actgtatggg acctctgaag caggcgggtg
      301 ctcgtttgat tgcacaacca ttggctgtat tcccggttgg tggattcttg ccagaattca
      361 aaatccgcga aagcctccga aagggtagcc catttaagct gggtggaggg aatgaggttc
      421 agggaatcaa tcactttggc cattttctgg aaaaacatcg ggccgtattc tctttggttg
      481 gtcatgatgc gcgttacata aaatttatct gaaggccaga tccttttttg tatgagatcg
      541 acctgtgact gaatgaggtc aatgatggct tgttcatctg ctgccgagtc cagcaagggc
      601 gcacagccgg tgccgatgca ccatagatta cgggccggat tatgcgtata aaaccgggtg
      661 gtcgtgtccg gcttaaaaat tccaaaatca ttgaggtcgt tgcaatgcgg agccaggctg
      721 cccgccccgg aaaggagttg aaatttgatt ttatttccat aatacaaacc cgtgatggta
      781 tcggtgtatt gccaccaatc tatcaatgaa ctgctgttgg ggggacatac ataataaatg
      841 aatcccccta cggttttact tggccgggca cccagggaat ccaacaggta ccattggtca
      901 gcgatatttc tgccagggtt gtttttactg cggggatcga tttcgatatt atcccggtat
      961 ggcgcattgg ccaattgttc gaatatattg ccgcccgtgg atatttcatc ctgtcttgca
     1021 ccaaaaacca aaccatcggg agtctggagg ttccattttg cattcttgct gtcaacgata
     1081 ttggccagct gaactaaatc ctttttagcc ttggcatacc gcaaaggttg attaaaatta
     1141 tcgttgggtt cgttgtgtga aacgatctga atatacagtt tgggaatagt ctgcgacgcc
     1201 agggtggtac agaacagcag ccaggttgta ataagagcaa tggaatgcat acaaaaaatc
     1261 gggattgctt gcctttggat gtcctttggg gcttaaagtt taatcaatac attccatttt
     1321 ttctgaccag gtacttaaag gtacggatcc tgtttttttt gccaaagcct gacggtttta
     1381 gtatataaaa aaagccgctc catgcccgaa gcggctttaa ttggtctaaa aggtaattat
     1441 ttatttacaa taatttctgc accgagcgcc tgtaccccgt ctgcggtcag aaatcctttg
     1501 gcaaatacgg tataaatttt accggcctcc agggtaaggg gatccaggtc cagggccacg
     1561 gtctgcgtac cggccacccg gacttcgaga tcgtaagtgc cggcatccag aggagtaaaa
     1621 gcggttcctg atttaaagga agtgttgggg aagactaccg cgccaccgtc tacggcaata
     1681 tccaccgccg gagcattggg tgacagatgg ataaagcgga catgggcctt gccggctgcc
     1741 ggtgtgctta ggtcatcggc gactacgacg gctgaaatct ttttcacgga atcgatgaca
     1801 aatacgctgt agcttttatc ctgttccagg gttacattgg cgttgatcac ggtagtggtg
     1861 gtgcctgcaa catttacttt taaattgcgg ctgcctgcat tcactttcag gtatccggtg
     1921 ttctgcggat aggtaagggc ggatgtgttt tgttttacat cgtccaccag gaggtcaact
     1981 cccggggcat caggagaagc atgaatgacc aggacattgg cctgggcaag gtcctcatcc
     2041 tttgagcagg aggaaaataa agtcgcagcc atcaataaaa tggccggagc aaaagccagc
     2101 atacgatttt tcatgatctg aagtttttgt tgttatttga ccaatcaaac ggaaagcctt
     2161 ggatttttgt ttaatctttt tttaaaaaag ttaaacaaaa ctatgaaatt aacatttgtt
     2221 caaaggtgcc atgggggaaa aacaagtcac acaatcgaat taaataattg attaacagtg
     2281 tttttaggaa aatggaaccc gttggtcatt aaccaaggct ctggaattta aaaaaatcca
     2341 tattcctggc agaaagcagg tccgtattgt catgagatga ctgaaataca gtaggctgga
     2401 accagcaggt cctaatctat tgctgtggca aagcaaaagc cagcaaatat cccgaagggt
     2461 tttctttgtc cccggcaacg gaggtgacca cgtattgttt tcccccgctc caataagtgc
     2521 atggtgtagc gttggcaaca cccggaaggt caatttccca taataattta ccgttgtcct
     2581 tatcaaaggc gcggaatttt ttgtcgtgcg tactcccgag aaatacaagt ccaccggcag
     2641 tgacgatggg gccgccatac ccttccgatc ccgtaatggg ttctccgggt ttctggtatt
     2701 cgggatgatt gcccaccgga atcttccagg catattcgcc ggtattgagg tcgatggcat
     2761 ttaattcacc ccagggtggt ttgatggcgg gacggccttc cggatccctg aaatgaccat
     2821 aggcggtgat gttgagataa agcgcggccg tatccgaagg ttcggattct gagctggagt
     2881 cttttgcggc cagcttgttt tccctgattt ccagcaaata cgattctttc cgtgagatcc
     2941 ggtctgcctt gtcgtacaga taggaaataa tggcttcttc atttccctgc attaccgcgg
     3001 caaatcccgg catcctcccg ctgcccttca ttattttttc cagggcggtt tcgcgatcca
     3061 tgcgggcgga caaaccaacc agggaaggaa aattgggttc atccccattc ttatctcttc
     3121 catgacaggc ggcacagtgt ttggtataca gggacatgcc ctggttatac ccggactttg
     3181 aacctgctgt tttttcggtt tctattttct gaagcagttc gatttccggg gaatccgaag
     3241 atttgacata gagaatcccg gtggccggat cgaaagcccc cccgccccaa ttggagcccc
     3301 ccctggttcc cggcaacatc agggtgcccc gggtgtccgg cggcgtaaac aatccctcgt
     3361 accgaaaact tcggaacctt ttcaacaggc tgtcataaga agagggggaa tggtacatta
     3421 aatccgcatc ggtcatcgat tgcctggaat agggtttggg ttttaacggg aaaggctggg
     3481 taggccaggc ctcttcgccc ggtaccaggg aagggggaac cggtctttct tcgacaggga
     3541 aaaggggcac tccggtttcg cggtcgaaaa caaagagaaa gccggatttg gtcgtttggg
     3601 ccaccgcatc cacttttttc ccatcttttt caagggtcac caggtttggg ggagcgggca
     3661 aatcatagtc ccacagatcg tgatgcaccg tttgaaaatg ccagatataa gccccggttt
     3721 gagcattaag ggcgaccacg ctgttgccgt aaagattttg acctttccgg tctgctccgt
     3781 aaaaatcata agttggggat cccagggcaa ggaagaccat gccccgtttt ttatccaggc
     3841 tcatgcccgc ccaatcgttc gcccctcctg catatttcca ggcatccctg ggccaggttt
     3901 cataacccgg ctcaccggga tgtggaatgg tatggaaagt ccattccagc tttccgctgc
     3961 gaatattata agcgcggata tagcccggtt cggccccgta caattccgag acttcggctc
     4021 cgatgatcaa taagtcccgg taaacaatgc cgggcgaggt agggatcaca gaaatggtgg
     4081 cgggatcgcc ccggaggccg atattcatgc tcactttacc gctgtcacca aatgggatga
     4141 ccggtttgcc ggtcagggca tggagggcaa acaaccggtc cccggcggta tacaaaatcc
     4201 ttttatcctc accgtcttcc caataagtca ctccccggtt gatgcctcct ccctcgcccc
     4261 cttcaaacgg atcatgggac cagatgattt ccccggtacc tgcattcagg gcgtacaaac
     4321 ggtgacgcgc ggaagtggtg tacagtatcc cgtcgatgac aataggattg cattcgctgc
     4381 tcccgatgcg gttgttgcgg gagtcgtttg gaatgaattt ccaggcaggc tgaaggagat
     4441 ggacgttgtc tttgttgatc tgtttcagcg gggaatagga cgaactttcc gcatcggctt
     4501 tatagacaga ccaggtgcgg tcgttattaa tatccggttg acaggcggat aaaaatagga
     4561 aggtaacaag gctgagaaac gatttgagtt tcacggaagg cataattgtt tgatggaacc
     4621 tggtcatgtt ttacggggca ggagtttaaa taattggcca aaaataggct tagcatagcc
     4681 ggactgcaaa tccgataaac ccctatcttc ccaaaatgta caatcccttt ggaggccagc
     4741 tcccgccgcg catccgatgt aataagtttg ttaactggtt aattgtaatc caatgccttt
     4801 acccatttca tggcatcgcc cagattgacc ctattcccgt atttgtttca ggtcaggacg
     4861 gacaccgttc ttaccggatc ccggccatcc tttctttgcc ggatggccag ttactggctt
     4921 tctgtgaagg ccgggttagc ggcagcggtg attttggaga tatcaatatc gtaatgaaac
     4981 gaagcccgga tcaggggaaa acctggtcag ccatgcagac cgtggtgaat tacgactccc
     5041 tccaggcagg caatcctgcg ccggtggtcg atctcctgga tcccgtgaac ccgggtggaa
     5101 gattgtttct gttttacaat accggtaaca accatgaaaa cgagatccga aagggaaacg
     5161 gcctgcggga agtctggtat ataacctcga cagacggagg gcagacctgg agccagccca
     5221 taaacattac cgcccaggtg catcgaccca tcaaatcgga agtaaattcg gaataccgtt
     5281 ttaaagagga ttggcggtct tatgccaata ctcccggcca tgccattcaa atcggctccg
     5341 gaaaatacag aggacgaatg tatatcgccg ccaatcactc ccagggagat cccaagcctc
     5401 attttgaaga ttatttcgcc catggttatt attccgatga tcatggcaag agcttcgtac
     5461 tcagcgaatc cattccaatt cccggaagca atgaagccac ggccgcagaa ctatccgggg
     5521 acggcctgtt gatgaatatc cgcaatcaaa aaggggacgt caaagcccgg atcgtggccc
     5581 ggagccggga tggaggaagt acctgggaca ccgtttattt tgacgccaat cttcctgatc
     5641 ccgtaaacga aggcgctctc ctgaatctcg ggaaaacagg aaaaaggcac cggcttgcct
     5701 tttccaatgc agccgatacc ctgcaccgca accggcttac tttgcgcatc agccgggacg
     5761 aaggtaaaag ttggtataaa aacatcctgg tcgatgccgt ttccggtgaa caggccaggg
     5821 accacaccgc ttattctgat cttgtaaaac tggaccgtaa aacggtaggt attttgtatg
     5881 aaaggagggg atattcgcag atcgtattcc gggcggtgaa ctggaaaaag gaataaagtc
     5941 aagtctgaca ggccgattct ccggaaagta aatttttata atttatactg cttccagaaa
     6001 cgcctgttcg atctttttca tagtcaaaac attttgccac tatccgggat tttggtaaac
     6061 ccgtacttca gataaaatcg ttccgcttca gcatccatgg ggtcaacgat gacggcaggc
     6121 ttcgtttcag tgcatccgca agcataaggc attattattt tgcctatggg ttcacctttt
     6181 ttaatttacc ttccttttat taacttataa aggggtgttg atatgaaaac catgctgatt
     6241 ttggttgttt tattttgttt agctgaccgg tcgaaagccc caagccctcc tgttcgtaaa
     6301 gctgatgtca ttatttacgg cggtacctct gcggccgtaa tcgccgcggt tcaggtgaaa
     6361 aaaatgggga agtcggtcat tgtcgtttca cccgaccatc atttaggcgg cttgtcatcc
     6421 agtggactgg ggtttaccga tactggaaat aaagaagtca tcggaggtct ttcccgtgaa
     6481 ttttatcaac gcctttaccg gtattaccag gattcttcga cctggaagtg gcagaaaaaa
     6541 gaggaatatg gcaacaaagg ccagggcacc ccggctatag acggggcaaa ccgcacgatg
     6601 tggatttttg aaccgcatgc agccgaaaaa gtttttgaag attttgtgaa agaatataaa
     6661 ctagaaatat atcgcaacga atggcttgac cgcacatcca aaggggtaat taaaaaatcg
     6721 ggaggcatcc gatcattcag gaccctgagt ggcaccaggt accggggaaa catgtttatt
     6781 gatgcgacct atgagggcga tcttctggca gctgcaggga tcagttatca tgtaggcaga
     6841 gaagccaata ctgtttatgg cgaaacatgg aacggggttc aggcaggggt tttccagcat
     6901 ggtcatcatt ttaaatcccg gatcagtcca tataaaattg ccaatgattc tttaagtgga
     6961 ctgttaccgg agatttcata cgaacccgtt gctccgaacg gatcgggtga ccgtaaaata
     7021 caagcgtatt gtttcaggat gtgcctgagt aatcatccgg acaaccggtt acccttccca
     7081 aaacctgaag gatacgattc ctcccgttat gaactccttg cccgcgtctt tgcatccgga
     7141 tggagggaaa cctttgacaa attcgatgct attcccaatc gcaaaacgga taccaacaat
     7201 cacggtccgt tcagcaccga ttatattggc aaaaattata attatcctga agcaagttat
     7261 gagcacaggg aacggatcat caaagagcat gaattatatc aaaaagggct gatgtatttt
     7321 ctgcaaaacg atcaccgcgt tccggaagaa gtccgggaaa aaatgaaaga atggggctta
     7381 ccaaaagatg aattcaccga taatggacac tggcctcacc aactctacat ccgtgaggcc
     7441 aggcggatga tcggcgaata tgtaatgaaa gaagccgatg ccttgggtaa aacccaggtc
     7501 ccggaaccga tcggtatggg atcctatgcg ttggatgcgc acaatgccca acgatatgtg
     7561 caatccaatg gttttgttca gaatgaaggc gatatcgggg tgcaccccga taaaccctat
     7621 tccattgcat accaatcgat tttaccaaaa gccaccgaat gtaaaaatct cctggtaccc
     7681 gtttgccttt ccagttctca tatcgcttat gggtccattc gaatggaacc agtttttatg
     7741 attttaggtc agtcggcagc cactgcagcc gtgctttcca tacagcataa ggtatcaccc
     7801 caaaaattaa attatgaaac ccttaaaacg gttttgctaa aagataaaca acgcctggag
     7861 aactaagaac ttcaggtgcg gttatgcacc ggccactatc cactaatcac ctcccgcggc
     7921 tgcgggacac tactcaccac ctgcctctct gatccaatat ctcgacctcg gagccatatt
     7981 caaaatcaat ccctcaatat tcgtagaaag gctgtctccc gtgagcacat tgacatagct
     8041 gcccgtcagg gagttgaatt tcacctgggc caatttgggg ttataattct gtaccaggaa
     8101 ttcccccttg ccaaaaggct ggaaacttac gcgggcaggt ccatccatgg gtatgatttc
     8161 atttccatta aagaccttac ggattacatt ggcccaggcc agcggtactt ccatcaggcc
     8221 cagcggtttg gggcagagca gttcttcacc ggtggattta aaatcctcat ccgaaaaggt
     8281 atggacattt aggacataga tattaccctt tgcattacga cataaaaaag ggatttcttg
     8341 tttattgaat atggctgtca gttcgacggt ggcatctttg ggttttatat ttccttccag
     8401 ggacaaggca aggtccagag tatcgggttt tccattgacc aggatttccg gggtagggat
     8461 gacgggggta tcgatgggcc attggatgcc ggccatttca gccagttcct gcccctggct
     8521 cagccggttg aggaagccgg tcgtgaggat taaacgtgat ccgctcgcca tggatttttt
     8581 aattttttca aagatggcgc tgtcctctgc agcctgggcg ggaaggaata ttatttcagc
     8641 atgggaggga taatgagcat aaggaatgag cgggacccca aacatgccaa tgaaatcata
     8701 gatatacata tcgccccccg gatcgctgtt gggcggtttg taggtgctga tcccgtcgac
     8761 cggcgttttg gacaagaggg cggcgaggtc ggcaaggtga ttaaactcgg accgcaggaa
     8821 atgctgcccg ggatggccct ccataaaatt gtaggaattg aaaaggatca gttcttttgc
     8881 cccggccagc accgcctggt aggcttgttc gataaaatcg ttctggtcgc agtcgatatg
     8941 gtcaaaccag gcgcctccca tcttgtcccc ggacaccgat gacatccagc ggtaattgac
     9001 aaatccttca taaggctgga cgtatccaaa acggggcgta tattgcccgc ggctttctgt
     9061 gccgatccat acctggtcgt acaaagcggt ttgccgggtt acatcatatc caaacaagtg
     9121 gtagcggtca taccattgcg ggtatttgat gatcacacgg atgtccggat tggctttttt
     9181 agcaggggcg atgaggtatt tttctgaaaa atcggtcagc aaatccctgc ggtaggctga
     9241 ccaggaccgg tcgcctttgg cggtttggga ttcaacgctg gtatccgccg tacagacaaa
     9301 atcatctaca ataaaggcat caaacaacgg agcggttttt tccatcaggg aagagaggtc
     9361 tttacgggtc ttttcatttt gccagttgaa ccactggaat ttgccatcct gctttaaacc
     9421 aaaatttcca cccggtacgg ttgcaatgcc ccctgcaacc tcaaagccat tggttttaaa
     9481 aaaccgaatg actgaatcca gccgggcatt ggacacgacc agccctgaac gatacacctc
     9541 aaggtacacg gaactgatcc cgtttgccct gagcatggaa agcgcctccc gcttgccggt
     9601 ttctcccgtc cacaactggt cgacggcctg ggcggtaaaa taaacgctca gtttgaggtc
     9661 gtttttacgc gaaagggcat agtggtggac atcctggtcc gtgtcggctt ccggtttgca
     9721 ggagaaccaa aagggaccgg caaaaaacaa atacaagaga aatttatttt ttttcatttg
     9781 ggaaaaggca acgaccggta agttagtaat ctcaaaagaa tcctgaaaga atgccgactg
     9841 gttttatatt gtattcaaaa tcagaaccac agccttgatc ggatgtctaa gtcattgtta
     9901 ttaaatcaaa ataaatgaaa tcaggaatac tcgtccttgt tttgaccctt gctgcaatgc
     9961 ggcctgtcct tgcacaagaa ggtaaaataa aaatatggaa tcccgccaca gattccattc
    10021 aatgcctgga aggacaggcc tgggcaggaa gtggaaagaa cgtttttgcc cggttgcctg
    10081 ccaacttgga aaacaaggtt aggaaagaag tctggaattt gtcccaacac accgcaggac
    10141 tgagcctgcg gttcaggacc aatgcttccg aactgaccgt ccgttatacc gtgaaaggag
    10201 gccatcaaat gccgcatatg ccggcaaccg gcgtgagcgg aatggattta tactccaaat
    10261 cgatcgatgg ccagtggtta tggtgcgcag gacgatacac ctttggagat accttgagtt
    10321 accggtttgt aaatctgccc tcaaaggatc aacacgtctc caattgggaa tatactttat
    10381 acctcccatt atacaattcc atcaaatgga tggaaatcag tatgcctgcg gaatgtgaat
    10441 ggagacccct tccggtccgg aatgaaaaac cggtcatcgt ttatggcacc tccatagccc
    10501 agggaggatg tgcaagccgc ccgggcctgg cctggaccaa tatcctggcg cgaaaactga
    10561 atagtccggt ggtcaacctg ggtttttccg gtaatggccg gttggaaaca gaaatccttg
    10621 agctgatggg aaaaacggat gcaaaggcct tcgtacttga ttgccttccc aacctgatgc
    10681 ccccggcttt tacgggggag gaggtgaaaa acaggatcca gcaagcgatc gggctgctga
    10741 ggaaggcccg gcccgaagta cccattcttc tgaccgacca cgatggctac acggatgaag
    10801 cgatgaatga agccagacgg aaggattata ccggagtcaa ccaggcactc catgaaatcc
    10861 tttattccat acgaaaggag gggatcggcc ggttatatca cctgcctaag gccgaaatca
    10921 accaggacat tgaaaccatg gtagacggcg tccacccgaa cgatatcggc atgatgcgtt
    10981 atgcggaggc ttatgaaagg attttgagaa agatcctgga catggaagac ggaggtaccg
    11041 tgactaccac agcggttacc cagcgccggg atgcccgtat ctatgactgg gagacccggc
    11101 acgaagaaat cctggattat aataaaaacc atcaaccaga gatcgtgttt ctgggcaatt
    11161 ccatcaccca tttctgggga ggcctgcccc cgaatccggt caacaatgga cctgaggcgt
    11221 ggatcaaaca tttcggaagc cggaggatag tcaatatggg attcggctgg gaccgggtgg
    11281 aaaatgtgct ctggagagtt catcatggca ccctggacgg cttcaaggca aaacagatcg
    11341 ttctgatgat aggcaccaat aatctacatc tcaataccga tgaagagatt gtcgaaggcc
    11401 ttcgttattt aatccaggct atccagtcca aacagccaca atcgacgatt cttctgatgg
    11461 gtattctgcc gcgcagaaat actgaagagc gggtggttca aataaataaa aagatagcca
    11521 acctggcatc agggaaaaat attcaatatg cggatgccgg acctttgttt gtaagggcag
    11581 atcaaaagat cgacgaggcc ttatttactg atgggctgca tccgaatgag atgggatatg
    11641 acaggttggg tggatttatt atacggtgga tggatcagta gacaggtttg ctccatcgct
    11701 gaagttcttt tattcgaccc gcttcggggt cgcaggatac gttgtgatat cccggtggct
    11761 tgtgccaccg gttattagta gttgacccct gcggggtcat ttgtcccaat ccgttggcga
    11821 agattatact atgagctaaa gcccccggag ttaccttgat aagcccgagg ttaaagcccc
    11881 ggggttggcc atactgaata tacagggcac taatgggtgg agttcacata actccagctt
    11941 tcaggctggg gttacgtgaa agacaattac aagattgggc tttagccccg gcccggcagg
    12001 atgtcagctg ttgatctaca tatattaagt aaccgtattt tcagcatgtg aatggtttta
    12061 atctgatatc tcttttggat ttattgggca cctttgcttt tgccatcagc gggatacggc
    12121 tggcttcggg taaaaatatg gattggtttg gggcctacgt tatcggactg gtcacggcca
    12181 taggcggagg aaccaccagg gatgtattgc tggatcttac tgctttttgg atcctggacc
    12241 cctcatactt gttgaccacc gcctttgcct tgctggttac tctcttgttc agggaaaaga
    12301 tcatgctttg gggaaaaccc ctgtttttgt tcgacaccat aggcttgggc ctgtttacca
    12361 ttgtaggtat cggaaagagc ctggaagccg gacttccagc ctgggcttgc atcatgatgg
    12421 ggactattac cggttcgctt ggaggggtga tccgcgatgt cctgatcaat gaggttccct
    12481 tgctgttcag gagggatatc tatgccctgg cttgtattgc gggcggaatc gtctttttta
    12541 tttgtcaaaa gacgggactt ggatctttct ccgaattggt tggcgccgct tcggtcattc
    12601 tcatccggat gcttgcggta aaatttcata ttcaccttcc aaccctaata cctgagcata
    12661 ttcgcaataa cagccctggg gactcatcgg aacaataacc cggcttattt tttcagaagc
    12721 catcggggga aaaaataaaa cctcttgcgc gatcctatat cttgataaat ttaaccgacc
    12781 tgattttatc ttctgagagg atggaaagat aataggctcc cgatggcaag gtctcaagat
    12841 ttaaggtatg gccctgatcc accagttccg gtaccggttg caggtaatgg atttttccgc
    12901 gggaatccac cacccggtat accatttttt cggacgccag cttttcaaac cggaaggtga
    12961 tggccgaaga ggcgggtacc gggaataatt caaatttatt caatgcgagt tctttgaggg
    13021 gggtggtgtt tttaaccgtg attttagcga ctccaaaata aaaaccgata ccgcattcgt
    13081 tggacaggct gctgatggtg tattccgtag tctgaaccgg ttttacaaaa aaggtataag
    13141 ggctggagga cagggtaatg gttgaatctt ttccaatgga gaccttccag ggcggcgtgc
    13201 cggtgagcgt gatggtaatg gggacgctgt ctccgggatt gatggaggcg gtgcctgata
    13261 tttctgcact ggctgactta gatacagtga tctgtgccgt gttggattta agggtggagg
    13321 acaggtcctt tacctcacaa taataaccgg cgctttccat tggagtgatc gaaagggagg
    13381 cttcggtggc atttgggatc aacatattgt ttttatacca ttggaagccg gtacctttcc
    13441 cgccctgggc ggtcacttcg agtacgatag gcttttgatc gcagacggat ttggactcgg
    13501 gatgccgggt gatcactagc ggggcgatga tcccggtcac cgccacggca tcaccgattt
    13561 gcgaatagaa ggtcccattg ccatgggtgc cgatcaccag ggtgttatcg gtccagcggt
    13621 ggtccatgct gctgatcacg gcgtattcca tgggtgaatt ttcattttct ttaaaccatt
    13681 gagtacccga tttgtcgatc atggtagtgc tgtataagcc tacggtggtg cccacaaaat
    13741 attccacccc cgattttttt acaatgattt cacaacttct gatgctgggc tctgcaagat
    13801 taccttctat atttatccag tttgggattg ccgcattggc atttccggtc caccagatgc
    13861 ttttgacgtt gtagttgctg tagaccacca tcagggtatc gtcgtttctt gggtttacgg
    13921 caatatctaa aatggagcct gtggccagca ctccgttttc ttttggagtg atattatcgg
    13981 gaatttgatt gggcggggcc atccggggat tccggagcct gaaaagcttg cctgctgaac
    14041 tgccataaaa aagattatgg aggttatcgt aagctccccg ggtgcttgcc atgcaaagga
    14101 tttgttcatt gtccccgatt tttgttcccg ggagcacttg ccaggtgctg tcggtcacca
    14161 tactggcctg ggtggtccgg taaaggacat tcgtacccgt gaaatacaag tcctccgtat
    14221 tgtcagggtt caggtaaatc agggttctga acgcgctggt gcttcctttg ggttgcaaca
    14281 ggccggaccg ggaaagaaag gcattgttgg cagggttgac catttgcctt aaacaataac
    14341 cgccttgtcc cgaaatcaat aaccacttgg tattgtttgt aattttagag atgccatggg
    14401 tcatgccgtc cccggtgtaa attctgattt gatttttcgg attgggtgat aaaccattat
    14461 aaaacagggt gccgttgtcc tgggtgccgc caataaaaga aagggaaccc ggattgggat
    14521 cgatcgcgat cgcgtaatac tgggtgtttt gcaatccggt attgatgtat tggtgtctga
    14581 cggtatcggc cgaaacctgg gtagtcaggg aaagtccccc atcgtgaaaa tggatcattt
    14641 cagaggaact gccaggcctg aaaataaaac catgaatgtc cggatgagac cggtccccgt
    14701 cataaccctg agtacttcct gccggcgcgt tgcggtaatt gaaaccgccc aacagccggg
    14761 ttgctgtttt ggaccggaaa ccactggcgc tgcggtaggc attggtgcct ccgatcagca
    14821 caaaatccgg atcatcgggc ttcacagcca gggcaagatt ataccccccg gatgtattca
    14881 atcccccaaa attttcattg gcggcattgg aaggcaaatt ctggctgagt tcggttacct
    14941 tattgccact catatccaat tcaattttaa ataaatctcc tgttggaaaa attaaattgg
    15001 gtccattcgg ttgatttacc gaaagggcat agagtatgga ttcgttcgag ggcgccagtg
    15061 cgatggagat ccggtaatat ttctttggag caggccaaat attaaataat acagagtcga
    15121 ctattttttt ccattgtatt gggactgcat tttcaccctc ccagataccc tggtaggtag
    15181 tgtttggaaa tttgacgggc acagcagtgg attgtccatt aaaagccaaa taaattttag
    15241 atcccgattt ggagattatt atatcatttg catcccatcc cccaacgtca ccattaccga
    15301 tttcaaaggc ctgttgaatg gaaaattccg ccagagactt tctattaagt tgactaaacc
    15361 ctccccattt cgaaacccag ataaagccat cttgaggatt gactaccagt ttgccaatat
    15421 aatcgtatct gttgtcgaat aaaaattcct tgccggcttg ggttattttc actggcatcc
    15481 aggttaaacc tttgtcgtcg ctgaccatca ctccgaaacc atgatgagta tgtccccaaa
    15541 ccgtcgccga accacctggt aattcgccgg tcccggcata ccaggtatcg cgatagggtt
    15601 taccggtgac tggatttatg cctacccggg gatcctgggc cagggcggtc acactgttca
    15661 aattcggggt aggaatccag gtccagttca ttcctccgtc cgtactcctg aatacaccgc
    15721 ccgaaacgcc ccctgccagg atcaaacggt tggaagtccc gttgtatccg atatcatagg
    15781 cagccgatct cacccggccg gcaatgttca cgggccctgc tgaaatataa gtctgtgaac
    15841 gaagagttct cttaaccctg ggtatagcct gtactgccaa acgatcccgg tttctggcat
    15901 tgaaaggcac ccggccggta gccgggtcgt ggctgatcat ccattcgtat tcctcccggt
    15961 ctttatgaaa ttcgggtttg gcccaggaag gggaagcaac cggtccggtc ctatgcgcgg
    16021 aaagtgattt ttccgagggt acaaaagggc tgaccacaaa atacagggca atcagggaag
    16081 aaatgaggat ccgcaagttg ccgtacgaat gattcctgat catgatgtaa gggcatttgg
    16141 taaggaaatt gggttaaggc ataatttgat ccggcaagtc cgtccaatgg tttatgatga
    16201 cttttccgat attgagttcc aaaaccagtt gatttttttc acaaaccgct tgattgatcc
    16261 ctgccgcagt ttccggcttt ttgagatgga ttaaaaaagc gggcaacttg tctttcggca
    16321 ataaagtatc ccggcgatac ccttccgggc catagatcgg ctcggtgata tagggttcct
    16381 ggaggcgttc cagtacagct ccatagatca tttggtccac acatatttga accacccgga
    16441 tctgggacga attattttgg ggtaaaagga aaaggtaata acggtacatg gaaacagccg
    16501 gtgatgaagg gtcgggggcg gctccgggaa tgaccttttg gcgaaaggcc attagattga
    16561 atttatttga tttcgcccgt gaacaggaaa gaaataaaaa caaaaaacaa aaaagggcag
    16621 aaaacaaacg catggctttt aaatataggg atttgttttg aaaagtcttt gaataaaaaa
    16681 acttgggtgc atatcaatcc cgcgccggtc tcgtttatcc gaaaatgtaa aacctgcatt
    16741 ctgtcatacc cccaaagcct tcaggattgg gcataccaaa atgaatcagg cctgattttc
    16801 agggctgacc actttgcgca agaacgactt cggtcttcac ttcctgtttc cgcaaatttg
    16861 aaaatctcgt cagatgcctt atttttgagc ctcaattcca ttttaaccat gtttaaacag
    16921 gtacttgctc tcaccctggc tttggccttt ctaattgcct gtaaatcaga acatccggac
    16981 cttaaaacag cctatgagct ctttaaggaa ggatttgagc agcattccta tatcattaca
    17041 cacagcgctg aattgaaaaa tgaagccaaa atggtagata gccggatccg taacgaagac
    17101 aaggaaagtg tgtttgccat ttcggaagta tacaatcatc tcgatgaagt agatggcggc
    17161 ctgaaaaatt tggttggccg gatccaggct gtcccgggtc atgaaaaaga atctttcggc
    17221 gccccggtcc tggataaaaa gatggaaccg gcccagattc tgatgatcca gaaaaattac
    17281 agggattcgg tactgcttat caaaaacgaa ctggacgcgg ccgccaccct gctcaatgcc
    17341 ctcaaaacaa aattgaatta agcccggttt tgaatctttg ggcccggttt ttatttttta
    17401 gcattttcat cctgtcctgt cgggaaaaac acgacatgga cctctatatt ccggaggtat
    17461 ttatcaatgc ccgccaggaa gtttacctga ttctcaaaaa ccagggcaat acattcatcc
    17521 cccatcgcaa tcttgagttg gaagtatgtt gggatgattc gtccatggtc cggtttaacc
    17581 tcgacagcat ggacccccac tttcgttatc cgggcgacag cagtatcttt caattgccgt
    17641 ttaaggtgca gcgctcccgg catttggtaa aagcccgcgt ggaccgattg aatcagcttg
    17701 ccgaatccga tgaagtccac aatgtttatt ccaggacgct gaaaacggat tttcctgaag
    17761 cccctgtccc cttgacttat ttcccgggat ccaaacgatt tgaaccggca aaatacctgg
    17821 acctgatttt acaagcccct tttgcgagcg gtattctctg gtacgaggac catgttgttt
    17881 accccgtggc ctattggagg aaagcctgga aagatcagct gatctcgaaa ctgggggcga
    17941 tatattcagg aaccccggtg cctcttccgg ataccttgcc ttggtccctg tcccgggacg
    18001 tggccttcgg ggtatattta aattatattg cgcacagcct ttggctggaa atggaaaaag
    18061 aagtgccctg gtctcttcgc ggcatgaatc ccgatgatct ttcgggatta tgggatgcac
    18121 ggcaattttt tgaatgggac agccttagcc gggaatacca tccgggatat caaatcagcg
    18181 gagccgtttc tccgcagcat cccttggtga gttaccggtt cgcagctttc ctcaacggcc
    18241 ttcccgaaga cattccgggc tccatcaggc gctttctgga ttggggaagg gcatacctgg
    18301 tccatgcaga agggaaagga ctgcccgtct ttcaaaccgc gaaggatatt tattatccgg
    18361 aatccggcca gttgcacgcc gtaaattcct gctgggccag cgggggcctg atggccgatt
    18421 attgccgcgc attcaatatt ccggtaaggc ggcacaatat tgaattgtcc aacggcatcc
    18481 acaaccagct tgaatttccc acggaagcct ggtacctggt acatgccgat gatatgtacg
    18541 accctttgtt ttatcccttg ccgggatcca tctcttccac ccggatgctg tttaacaaat
    18601 ccgaattcaa tcagatgctc cgcggccggc cccattgcct ggaagacagt tgccacagca
    18661 aggggaccca gctttccttt gaacgcaggc ggaacctgct gagcaaagcc cttgatttga
    18721 attcaggtta tttaatgctg cagtacgacc agggcaggaa caaattcagg gaatttatcc
    18781 ggggcgaccg ttttgaaaaa ttcctggttc cgctttttaa tgaggaggaa gaacgggcca
    18841 tgatggaaaa aataaaagcg gccaaaccgg attatcattt cctttcaacc ctgtacgagc
    18901 gttttgaagc gtgtaaaaac aacaggagat agtcaatttt atttgaagga ggccagtgcg
    18961 aaatagacta acttcaaaat aaatttgcgc acttttttac aatccgatat atgaaagtaa
    19021 tgaaattcgg gggcacttca gtgggtaaac ccgaacgcat gatggaggtg gctaggctga
    19081 tcaccgcgga ccgcgaaagc aaaatcgtgg ttttgagcgc cctcagcggc acgaccaaca
    19141 aactggttga aatcagcact tgtattgccc aaagggacaa ggccagggcc aagaccgaga
    19201 tcgaactcat ggaagcccat taccaggcct ttgtccggga cttgctccac acggaaccgg
    19261 ctatcgcaaa agccacggag gtcctgaggg aacattttga atttctgaat atcgccacga
    19321 agatttcata cagcgaagcg ctgaccaagg atatcctggc ccagggagaa ttgttgtcca
    19381 ccaaattgtt cagctgttac ctggaagaac aaaagatcgg acaccacctt ctttccgcct
    19441 tcgattttat gtccatcgat gaaaacgacg aacctcatat ttcggtcatc cggagcaagc
    19501 tggtgcccat cctggccgac aaaagcgaca gccagatcta cctgacccag gggtatatct
    19561 gtctcaatgc ccgcggcgag gtggacaacc tcaaacgcgg aggcagcgat tacagcgcct
    19621 ccctgatcgc ggcagccatc caggcttcgg tctgcgaaat ctggaccgat atcgacggca
    19681 tgcataacaa cgatccgcgc atcgtcaaga ccacccggcc ggtggaacaa ctgagttttg
    19741 atgaagccgc cgagcttgcc tatttcggcg ccaagatcct tcaccccgca agcatctggc
    19801 ctgcgcagca ggaacacatc cctgtgaaat tattgaacac tctggcgccc caggccaagg
    19861 gcaccctgat cgccaaatcc gcatcgggcc agggcgtcaa ggccgtggcg gccaaagacg
    19921 gtattgtcgc catcaatatc aaaagcagcc gcatgctgct ggcttatggc ttcctgagaa
    19981 gcgtcttttc catttttgaa aaataccgca cttccataga tatgatcacc acttccgaag
    20041 tggccgtatc gatgacgatc gaccagaccg gcagcctgaa tcagatcacc gctgaactcg
    20101 agaaactcgg taccgtatcc gtgattacca accaaaccat tgtttccatt gttggccata
    20161 atctgatcca gtcccccaac ctcctccggg acctgtttgc cgggattgcc catatccctg
    20221 tgagcatggt cagttatggg ggaagtccgc ataatatatc cctgctggtg ccggcggatc
    20281 agaaacatga aacccttcaa tcattaaaca ggcatttatt tggtttagat taacagtttt
    20341 aactattcat ttgctaatcc attaataatc aagcgaatag aacattttac ttttgcaggc
    20401 aaatattaac aaaaatgaaa aaaatccaaa ttcttaccgt tttaatgctg gttctcaccg
    20461 tttttgcctg taaaaacacc ggttctgaag ccgccgcccc tgcaatggat aatacttcgg
    20521 tagccgctcc cgaagccgcc cctgcagtgg ataccatggc tgccgcccag gatacgagca
    20581 tgcaaaaata aaccggattc tcagccgcgg aggtatgttg gaacggcatt ccgcctttct
    20641 gtatacagcc aaggtgaaac gtattcaatt taaaacttaa actttaaata caatgaaaca
    20701 gattctgtta ttcgcctttt tcgccctggt ttttgcggtt gcctgcaaga ataccggtac
    20761 cgaagccgct gctccggctg ccgataccac gatgatgacc actactgaaa ccatgactgc
    20821 cgctcccgac accatggcca tggctgcatt tgcctgcccc atgcatcctg aagtgaccgg
    20881 aaaagaagga gacaaatgtt ccaagtgcgg catggacctc gtcgccgtaa aaaaagagga
    20941 gccgggacat gaaggacatg atcattgatt agtggtcagt ggtcagtaaa cagtgatcag
    21001 tgatcagggg tcggaagacc ggtagattag caatacacac ttttattata caaaggctgt
    21061 tgaaagacgg cctttggtgt tttggagcac acggagagga tcgtgtctaa tgcgcacgga
    21121 ggtcgcggag ggcacggaga ttatttattt taatatttag tagccatgta ctgttgaagc
    21181 ggtccccaat aacaaatacg gccaaccaag gggctgacta aaaagtcttt gattcgcgaa
    21241 agcaacaaag ccacaatggt tacgtcaaag catggcttcg gcgctaccct atttatattc
    21301 ttcactggtc ccctggcatc ggagatgcct tatatttcta gaacctgata atggggcgat
    21361 taagggagaa gcccaacaga gtaggcataa gttgcaagaa ataaaaataa cttttaacat
    21421 gcgcgtttta ggcgtgtttg ttatgtcgaa gcttagcttc ggcctatgca attatagtga
    21481 tcaccgattg aaaaagtaca aggtcggagc tcagctcctc ccaaacgtat gctgaggcgc
    21541 acggaggtcg gggagggcac ggagattata aaattatagg tttgatatta gacctaacga
    21601 ttgataaatt ataaggtcgg agctaagctc ctcccaaaca ggttagggag acaattattt
    21661 tggtctgatt gaattttagg tcagcctgac aatttccaac aaaaattttc ttgccgactt
    21721 tttcccctta acttacatta tcctttacac catgcaaaca ccgatacccc atggccaatc
    21781 taatatttat cagttaccgc cgctcggaca tccccattga gcaggtcaat cttattcatg
    21841 aaggactgga aaatgtattt ggagagggtt cggtatttct ggataccgcg gatattcacg
    21901 gtggcgccaa atggaaaaaa atattgaacg aggcgggtac ccatgccaaa gtatgcctgg
    21961 ttttaatcgg gccccggtgg atggaaaaag atgaccaggg caggatccgg atccagcagg
    22021 aaggggattg ggtgaggcag gaaatcgagt atgccatgga gaaaaacctg gtgattctgc
    22081 cggttttggt caatggagga gtattgccgc agaaagaaga cttgccggat tcgctgaaat
    22141 cgttcaacga ttcacaggga attgtggtcc ggacggcgga gtggtctacg tataaggaaa
    22201 aactgatcag ggacctcaaa aaaaatataa aagggaccag caaacttaaa agcctgttgt
    22261 ggattttatt gccggcggca gcagtggtgg gaggatcttt tttattgcct aatttcataa
    22321 aacgtgacaa tacccaagcg ccaccttgta cgtcatttgc tacaaatgcg gaattaaaaa
    22381 ccctgctttt cccggtatat gcccgtgacc gacattcctc gcaggaaact atacagttga
    22441 ttgatgaatc cttttccacc aaatgcacca aatacggcat aagttttgaa aatcaattgg
    22501 caaacacccg cttcgatacg attattgtca gaagcagtaa gattcagatt gcaaagacct
    22561 gcggagctga tttgtatttt agcggacgtt gggtcggaga gcccggacaa caaggatttg
    22621 aggcagactt tcaactggtc agggacacca tcaatgcttt tgtcgcggca gcttccgatc
    22681 tcagtataaa actgacccgc ttcagtgtta acgaattgtt aggagatgaa gccgttgaag
    22741 aatggtacga aaaagtcatt caattcctgg tggggctcgt agcttatcag gaaggggatt
    22801 ttaaacaatc cgtgctagct ttgaaagagg cagtttccga tacgctgcgg caggattccc
    22861 tcaaattgct ggcttattcg atcatcacag atgcctatta tcaattgaaa atgccggatt
    22921 cctgcctgga ataccagcaa aagctgaccc gctggtcacc cactgaacaa gtagtgttaa
    22981 aaacggccat ccttgcggaa cagtataata ggccggaggt cgctattgta tcctatacca
    23041 ccttgatcga cagcggcaag tacgataaaa atgttttact tgaaaagcgg gctgaccaat
    23101 acgctgaaat caaggaatat tcaaaagcga aacaggatta ccagcgtgtc aaaaccaacg
    23161 aaaacaacaa ggaaagggtc aatgccaaaa tagaaagaac aaatacccaa atcgaacgta
    23221 accgtgcggc ggtcacctcg gtaaacaacc tccaggcaac tgaaaccgtg aaaatcaggg
    23281 ccgcggacct ctatcttcaa aacggacagg ccaataaggc cgctgaattg ctgaaaagca
    23341 ttcccaaaaa ctcagaatat tttaaacagg cggagccact tctgcaggaa gcacagttaa
    23401 agattaatcc ttccgcggta accgtgactg aagcggcagt caaagcgaat caaaggctga
    23461 aaacggaggc gggattgagg aacctggcag tcaaaaagaa cgattaattg tgaaaattga
    23521 attattcgat ctttatcacc accaggtccc acaatgccgt aaacaaagag aattcaagaa
    23581 cggaaacaga gttgttctga actttagtgt ataaccatca gttttccggt cgcatgttca
    23641 ggcgtttgtc ccggagcatt aaaaacaagg gtatagtaat agataccatt tgaaaaatta
    23701 ccggcggata aatcggtggt gtaaaccccc gggcttttaa tacccgcatc ataaaccgct
    23761 acggtacgtc ccgtgaaatc ctggatgctt aataaaacgc tggcttcgac cgggacttca
    23821 taggcaaacc tgacgtattc cgaaaatgga tttggataaa cggaaaccgt ttcccccttt
    23881 accttttgaa gttttcccgg gacccggtca ggtacaatta atgaagaggt tgagcaatcg
    23941 ttcaggtcgt cgttgtgagc aagatgcgct tttaaggagg ccgcggcggc acaaatgata
    24001 tgcccattgt gacagagacg caccttgttt gaatgtgggc cacaaagcac gaataccgtg
    24061 acctggtcac tcccggtaca acccagggag tccgttacag tcagggtata ggtggtggtg
    24121 gtctgtggac atattaaatt aaccatggaa tccggggaag cccctccgga ccaggcaaat
    24181 tgataaggct ctgccccgcc ggctgcctga gcggtcaatg cagtgcattc cgatccgtat
    24241 acgacggtct ggtcaggacc cgcatcggcg gcaatgaaaa attcgaaatc ataaatggtg
    24301 gtcgccccat tgatcccaat tctgaaatta tctatataag ctctgaaatc tgtgccaaaa
    24361 atacccacag ggcctccgac ggttagccgg atgccaccgc tccccattgc cccctgattg
    24421 actattctgg ctgttgggta tttctgtaca aaatcggcaa gggtgaacaa ggggccatta
    24481 tcttcaggcg ggatacctgc ccaccaagcg ccgttggctg catcccaggt ttgccagaca
    24541 ttatccagga tcaggccctg gtccggaacc tgggcgtttt gggcataaga accggtttga
    24601 tacaccggat taaataccag gttggcatcc aaggcattat ccccggaagt atcgatttgc
    24661 aaaacgatat aaggagcgtc gaccatctgg cttcggttgg ctataatagc ggaaaagctc
    24721 aattcggtaa actccgacaa caagcggtga tgatgaaggg tgttccgaaa acggacatat
    24781 ccctgcgggg agtacatctc caggctgcct ttgcccatga aggaattctg cggcgcgtta
    24841 ataaaattga ttttaccatt cggatctaaa aattgtttta cccagcccgc atggttacat
    24901 tctgtaaggg ttacgaccgt ctgggaaaga agtggaggtc cggcccaaag cgagagatta
    24961 aacaaaagga atgcaaaacg ttgtttcatt ttcataataa taggtattaa ggagttaaac
    25021 aattcatgga gaataaaacc gggtttgcaa aacggaaagt aaatccctgt ccgaacccta
    25081 gctgggtggg ggaatattaa attgatatct gctgtctggt ttaataagac atacggaaaa
    25141 tcgcaccaga tgagtcataa ttttcaaaat ccggacttaa atcataatgt taatttattg
    25201 aaaaaggtca ggggttttta attttatttt taaaaaagtc attaattaaa ttaaaataga
    25261 agcagtcaaa tccttattcc ctaattcaag acgactatat tttattatcg gtggggtata
    25321 ttcacgcacg gcaattgttt cgtatcttca taggctcccc gggcacaccg attttccttt
    25381 atactatata aataggttac gttttgggac ttggttacat tttatattcg gaaataggga
    25441 ttagccatgg taaacaacct ccaggccact gaaaccgtaa aaatcagggc cgcggacctc
    25501 tatcttcaaa acggacaggc caataaagcc gctgaattgc tgaaaagcat tcccaaaacc
    25561 tcagaatatt ttaaacaggc ggagcccctt ctgcaggaag cacagttgaa actaaatcct
    25621 gcttcggtga ccgtgaccga atcggccgtc aaggtcaatc caagattgag aacagaagcg
    25681 aacttaaaaa accaggtgat caggaaggta aactaaaccg cttatcccaa aactttggaa
    25741 tcagaccggt ttttcataaa tccagtatgt cttatagcgg atggcgccga ttttttcaac
    25801 aatgctgatc agtaccgcat tatcctctga tatccaggaa agttctgatt tggtatatcc
    25861 cctttccagg cccttttgaa tagtataata ataaaagagg atatcaaggc cctttcggcg
    25921 gtattcttcc gccactccaa gcatcaataa gcggaaactt tttatttttc ttttataata
    25981 aaaccatttc aagattcccg gcagatccag cctgcctttc atatggatca ggatagtatt
    26041 gatatcgggg atggtaagag agcaaccgac caccttatcc ttgaactccg caaaaaaaat
    26101 catttcagga tcgacgattt gttttaaggt ctcccccaca taatacatct cttcaatgga
    26161 agaaggaaca aacccccagg tctccttcat aaaatcattg aacagggtat ttatattagc
    26221 cagttccacg tgataatttt taagatttac ggtccggaac cgcactcctt ctttcttcat
    26281 tgttttttca ctgatgcctt gcatgcgttg caagagggcc ccattttgtt ttaccagctc
    26341 ttcggtcatc aggtaggcgt aaagatcatg agcttttgaa aagccggcct gttcaaacaa
    26401 atgccggtaa tacggcggag tgtgattcat ctgcataaac ggcgggctgt caaatcccgt
    26461 gagcagtata ccgcattcgt ttgctatgga gaaattcatt ggtccataga cccggttcag
    26521 gcctttctgg cgcagccatt ccaaggcctt atcaaaaagg gcggacgata cggcagaatc
    26581 ctccctgctc tcaaaaaatc ccaggaagcc ggcttgctcc cggtgcaatt cattatatcc
    26641 tgaatcgata taagccagga ttctgcctaa agcttccttt ttatccagga taagccaggc
    26701 tttgtaaatg caatggttga agtaaggatt gtttttccgg tccagggtgc ggcgctgttc
    26761 catgcgcaag ggggacaccc aacaggggtc gtttttatat aaactaaaag gaaggttaaa
    26821 aaacaaattc cagtcctgcc tggttaatac ttccctggtc tccatattat ggattggtct
    26881 gcctggacag ccggtttctt tccctgagaa tgatcgaatc cgtaagccgg ttgaacagac
    26941 ctggaaaatt atttttcatc cagcgcacga aacggctgtt gaaatcgcaa tagatttcga
    27001 acttgttttt tagtaaccca tccataaaaa tcttcgcgac acgctcagct gtgattgctt
    27061 taatgttttt atttaaggcg atgctttcag ggaggctgtg ttttcgttcg tattccaaca
    27121 agggggtatc ggtgtccggc ggataaatga tgcatatgcg gatgccgtca tcctttgcct
    27181 ccaggcgcaa acattccacc aggccggtca tggcaaattt aggcggggca taaccgctgt
    27241 atccaatgac accggtatac cccgccacgc tggatacaaa agccatcaag cctttgtttt
    27301 ttttcaacca gggccaggct gctttcgtgg catacacggt acccaggtaa ttggtaaaca
    27361 ggcatgcctc caggtcctga ataggttgat cggcaaaccg gccgcaggtg atgatcccgg
    27421 cactgtttat gagcacatcg atcccattga accggtcaac gattttaaga ataaccatat
    27481 tgatctgttc cctgtccgat atatctgccg ggtaatagaa aataccagaa ggaagaccac
    27541 tgattttttc cagttctttc tttgcctgaa tcagtttttc ttcatttcgg ctgatcagta
    27601 ccgtttttgc tttacgcagg gcaaattctt ttgccacagc cagtccaata ccggaagagc
    27661 cgcctgtgat aaccactact ttctcttcat actcatggtc gatcatgatt gttttatgac
    27721 ggtggtatat aattcttcca gtgcctccgc aaattgatca atgcggtctg ctgattgggc
    27781 agcggtaccg cacagtcgaa ggatggctga gttaaggggc accgcagggt agatgaccgc
    27841 agtggccagg aacccctgat ctaaaagccc tttggacaat ttggcggcat cttcattttt
    27901 accacaactg accgggatga tcgggctttg tgtggagcct gtaaggattc cgcccttacg
    27961 taatccggtc atcatccggc tggtgttttg ccataattta tttaacctcc aggtttcttt
    28021 atcaataatt tccagggact tgagggcagc tgctgaattg gccggcgata aggcagccga
    28081 aaacatgtag ggcgccccac cgtgtttgag ataaaggatg acttcctctt ttgcagaaac
    28141 gaatcctcca ttggcgggta tcgctttgga cagggaaccg gtgaaaagat caatccggcc
    28201 gggatcaatg ccaaaatgtt cagcgacccc tcgtcctgtt tttcccagaa cgcccaggga
    28261 atgtgcctca tcgaccatca ggaatgcacg ataggcctct ttgagggcaa ggatttcagg
    28321 caagggacag atatccccgt ccatggaata aaccccttcg accaggatca atttcctttt
    28381 tacccggtca tttttttcca aaacagattt taatgaaagg gggtcattat gcctgaaagg
    28441 ttccggttga atgcctgcca cttttaacgc atcgacgata ctccggtgaa tgcgctcgtc
    28501 ggcaatgacc aggtcgtttt tgctgaacag gccggcgatg accgcgaggt tggccatata
    28561 gccggaactc aaaaccatcg aagattcggt tcccataaac cggcttagtg atttttccag
    28621 gtgatggtgc agcacattgg tcccggtcag taatcgtaca ccaccggtgc cggttccgta
    28681 ctcatgaatg gcatcgatag cagcggattc aatttccgga tgaccgatca aacccaggta
    28741 atcgtatgag gacatcatat aaacgggctt gtcccggatg ttaacctttg ggcctgattt
    28801 tccttttatg acttcctgga aggtatagat atcctcatcg gcgcccagct ttaaaagttc
    28861 aaaccagcga tgcgccaatg cggtggtcaa ggaggagatg atcccgggat ctgaaattgg
    28921 gttttctgtt aaaaaactct tttaccaggt attgattttg ttttccttca agattgagtg
    28981 ttttcataaa ataaaatata gcccagggcg aacttcattg cagtcattga ggagcaaaac
    29041 tcaaatccat tgcctttaaa ttagcaaatt cattttgtta gtcctacatt tattttggca
    29101 ttattttttt tcaattatgc tattgataca acttacaggt ttatcggggt ccggcaaatc
    29161 aactatagcg gatggcacga gaacgaggtt gttggaaaag ggattacccg tggaggttat
    29221 cgatggcgac atttaccgta ataaattata tcctgagctg tgtttttccc gggaagaacg
    29281 ttgtgaaaat atccggcgcc tggcttggct ggcaaaccgt tttgtttccc acggggtcat
    29341 caccttgttg gcagcaataa acccatttga agaagtgcga agggaaataa aggccaacgg
    29401 gccccatgtc aaaacagtct ggatccgctg tgatctggaa acgcttatga aaagggacac
    29461 caaacaactt tacagaagag ccttccttcc cgaaggccat ccggataaaa ttttaaatct
    29521 aacgggggtc aatgatccat ttgaaatccc ggagaaagcc gatctcattg tggacaccac
    29581 tttcgaggtc gaagaaatca gtattgggaa attgtatcat tttatattaa atacactgga
    29641 aaccgcctga tggttaaaga aaaattattc cccttaacgc tttatatggt catgaaaatt
    29701 gccaaagaga atggtgaata accggagtgt tatactcaac ttcatggaca gcacctcttt
    29761 ggattgatca aaatgctcgt cgtttccggt tagaaagaat acctgactgt ataaataatg
    29821 taacaacacg gtaacaaaat tcaccccctg ttacacttat atataaccct aatcatcggc
    29881 tagtattcat tgattaaagt taaaccaggt aatatgaaag ttattctatg cttttgcctt
    29941 ctcatcttaa caaattgtgg gacagtttca aaattgccta aaactcaagg agacccatta
    30001 aatgtaaatt cttatggtta ccatccactg gatcctcttc ccgttgaatt ggaggcggat
    30061 acggcaaacc cattgaccaa ttcaagattg ttgaaagcca tgccagatga aaccatacgg
    30121 ctggccattg gccaggtaat cagtaccgga ggattaaaat ttacaacggc ttcagccgga
    30181 tacgagggta atagttatgt ggttgtattg gattatataa aattcaacac caattctttt
    30241 aatgtaaaag tggatagatc aaatggcagg gcaaccaagg cgaatgtatt ggaacagagt
    30301 tcccaggagg gcgatgcaat tattccttta tatattggcg tgggtttgag attaacagcc
    30361 aacattacca ttaataaggg gcaagtggat ttaggcaatt tatttgcgat tggtgcgcag
    30421 gcagcatcac aaaggatttc aggtaccttg acggtccaga ccctgggaat tagcggggag
    30481 aatatttctg ctgtcattcc catgcccagt gaaataaaca cgactaccat tcagaatgcc
    30541 atccttgcat tgggcatgat aaaagctaaa atttatgaag acaatacgac catcactcca
    30601 agggttgtgg gcgtatataa taacattgga gggtctactg aagtgatcaa tggatttata
    30661 tcttccattt tgcaaaaacc taaaatattg gcggttaaat aatgatttcc ggctcgtccc
    30721 tggtcgtagg aaggcggagc aaatctgcgg aaatagagtc tgtgtagcct tttttggttt
    30781 tcatctcagc tccggggcta aagcccccgg ttgaatgatt atctggattt aatggttccg
    30841 ttcaaaaggg gtctatcctt tcgcttgcat tatggcttat ctcataaaac cggaccgcat
    30901 tgatccaacg agtgatttta ggattgtgaa tttggccaaa cggcccagaa ataatcctgt
    30961 tttattttac gttcagtttg tataggactt ttttcttaat tcttatcttt aagagaccac
    31021 aataccccca ttataattac ccgttatctg atgtttttac cggtaccttt tgtaccccgg
    31081 ttgtacattg atctaaaaca gcattaaatc atttaatgaa tgaaaacaat caaaatactg
    31141 ggcaggccgg tcctgaaaga agaagggggg atcgaagccc ttcaaagcca gttcaacaag
    31201 gtggaaaaat cgttccgcct ggaatccctt cgcggggaaa cgaaggtcat ggataccgga
    31261 gcaaaagccg gggatatcat tgccctggaa tttgaagacg ggggagaatg gattggatcc
    31321 gcagacgata ttgaagagat ctttgggata ccgcaacccg caaagcgtgg ggcgggggcg
    31381 gatattttcc cttcacgcct ggccgtgaat tccggggaaa ggggtttgca ggatatcggg
    31441 atcaaattcc tgcacctgat ccggggcaag ggaacaaaaa gcatcagcca ggcggcggca
    31501 aaaaaactcg gacaaaccgc tgatgaaaaa atgatgccca aacccggctt gttcatggtc
    31561 aatgggcagg gtatccttac tccggtgaaa accgggttga aaccggataa atatttattg
    31621 tttattcatg gcaccctgag ttcttttcac ggttcatttg gagatttatt tgataaggag
    31681 tcagaaaatg acctcggcca aacaatcatc aggcaatacc aaaacaaggt attggccctg
    31741 caacatttta ctttatccca aagcccgttt gataacgcgc tggaggtact aaaactttta
    31801 cccaaaggat ccagcctgga catcctctcc cattcccgcg gaggcctggt ggccgatatc
    31861 ctggcctgct gcgataaaag aaatgttttg aaagggtatt ccgctgagaa tattaccctt
    31921 gcccgggatc aggatgaatc cctggcaaaa acgctggaac aaatcaatgc gctcgctacc
    31981 cgtaaagaaa tcatcgtgga aaaagtgatc cggattgcct gcccggcaaa tggcacactc
    32041 cttctgagca accggctgga ccatttcctc aatggatttt tgcgggcgat cggcttattg
    32101 gccggtgggc gatctaatcc tgtttatccg tttatccgcg gattgattac cgatgtcatc
    32161 cgcgcccgcc tgaccccggg cattttaccc ggcctttggt ccatggtgcc cgaatcaacg
    32221 ccccaacggc tcatcaaccg cccggaggtg atgctctcca accatctgct ggtgatcgaa
    32281 ggggatgcgg aaaaaggaaa aaacttctgg caatccatcc aggtagtgct caccaacctt
    32341 tattacaggg aagacaatga ttttgtagtc cataccggca gcatgcgtta tggagccctg
    32401 cgccagtcag gcctttattt ttttctttcc aaagacgaag acacctccca ttttaattat
    32461 tttaaaaacg aaaacaccag actggccatt caggacgccc taaacacccc tcccggacag
    32521 cctgtgcgaa gattcgagta ccgggccgcc tctgaaaggg atcgcggggt attggtggac
    32581 ctgtttgtga gcctgaaagc catttccgcc gatcgtattt cagggaacaa accggtggtg
    32641 atcctccttc cgggcattat gggaacccat ctgtccgtca atgggcagat catctgggca
    32701 gaccttgggg agattggcaa gggaaggatc gtaacggatc ttcatgccgg caattacgat
    32761 gtaggcacca cccatatcat cggcgatttt taccaaaaac tggtcggata cctcggtaag
    32821 gattacgatg tgtacaccat ggggtacgac tggagacaga ccctcgccca ggcggttggg
    32881 aaattaaata ccctccttga ggatatacgg cagcgggctc ctttacaaac tatctcgatc
    32941 gtagcccact ccatgggagg gcttgtggta cgggatatga tgaggttgca tccggatcca
    33001 tggtctgcct atatcgaacg accgggttca cgggtgatct tgttgggaac cccctggctg
    33061 ggctcccatc tgatcatgga agtgtttacc ggccatcaaa gccgtgtgcg tcagcttagc
    33121 tttttggaca cccggcacag gcgggatgaa ctgatccgcg tttttaacca atatcccggg
    33181 gtgtatgaat tattacccat taatgaagaa cctttcgaaa aagacagttt ctggaattca
    33241 atcaatgcgg aactggaatc ggacaagatc cttttgcctc ctttattaaa tgaatttaac
    33301 cggtataaaa accaggtaaa ggcgttcagt aaaaatccag acctgaaaaa catctattat
    33361 atagccggta aaagcgatca taccacctgc gcttaccgga tccggaagaa ctttttcggg
    33421 aagaggctgc aatacctggg cacgccggaa ggagatggat cggtgacctg gatgctgggc
    33481 attcccaaaa cactgccggt cgaaaggctt tattatgcac ccgacaccgg ccatggagac
    33541 ctggccaacg atgaaagttt gtttccgggc atccgtgaaa ttcttacaaa cggcgccacc
    33601 cgtcagatca gccagcaacg tcctgtctta cctgccatcc gttcggtcat gaacaaggaa
    33661 ggaatacaaa tcatcgaacc catgccggat accctggtat caaaccgtgc ggatgaagcc
    33721 tattccattc ttcttgaccg gcccagggcc acttcccgcc cggcgcggtc taacacccgg
    33781 atccttgagg tatcggtagt ccacggagat cttcggatag cgagtcatcc ggtgatggcc
    33841 ggacattttg tacgcgatgg catcaccaat gccgaggcgg cgattgacta ttatttcgac
    33901 cgtaaacttt ccgaaaggca ccggctttcg ggttatccgg aacaagtggg agagagcctt
    33961 gttttgtttg atccggtcaa atatcccaaa ggaacggtga ttctcggatt gggagaaagc
    34021 acggccctga ccccgtttca ccttcggaaa accgtggaag ccggcatcat cagttatgcc
    34081 atgcatttca gggataatgt aagtaccgca attgacggcc acgatacggc cgtcagttgc
    34141 ctgtgtattg gcaccggtta cggtaggtta ccgatggaag aagccctgca tgcgatcgtc
    34201 acgggggtca tccgcgctaa tcaacagact gaaaccctca atggccttaa agcgataaaa
    34261 aaaatcgaat ttatcgagct ctatgaacac attgcccaga atgcttatta ccagttgtcc
    34321 caactggaaa ataaccaaac ctcaggtccg gccttcaaac ttaaaaaggg aattgagaag
    34381 aaggcggggg ccaggcgcaa gtttcaattc agcaatgaaa attcctggtg gcaccatttt
    34441 accacccgcc tgatgggaaa ggatgaaacc agtaaaatac ccaggctgca attcacttcc
    34501 gcttccggca ttgcccgcgt ggaagaagaa aacaccttta cttccggaaa gatcattcag
    34561 gccctgctgg atcagatggc cgtggaccag ggtctctgga acaaggaata ttcaaaaacc
    34621 ctttttgaga tcctggtgcc caatcccttt aaagagatca tccgccacca aaacaatatc
    34681 ttatggaaaa tggacctgga taccgcttct tttccctggg aattgtttca cgactatgag
    34741 acggattcca tgcccgcctt tgcaagggcc ggtatgatcc gccagttgta taccccggac
    34801 acagaggcca gcccggacat tgtacgatgt cagaaagccc tggtcatcgc cgacccgcaa
    34861 tacagcgcgg atgggcctgg ccagttgcct tatgcccggg ccgaaggaat ccaggtgagc
    34921 aaggcgttaa aagagggtgg ctttgaaacc gaatccctga tcaaccggag cggattggat
    34981 attctaaaca acctgtacaa caaagaatac aaaatcctgc atatagccgg tcacgggact
    35041 atttcagaca accctcatga aacggggatc gtactggaaa acggcatgta cattactccg
    35101 gacatgctca aaaatttaag ccggctgccc gagtttgtct tcataaattg ttgttactcg
    35161 gggacggtgg agccgggtaa agaaaaacag taccagcagc ggtaccgctt tgcggccaat
    35221 gtaggcactc agcttatccg tatgggtgtg aacgccgtcg tagtagccgg ctggccggtc
    35281 aatgatgcag cagcaggtct gtttgcggaa aaattgtacc ggagcatgct ggccggaaaa
    35341 acctttggag atgccgtgcg cctttcccgc gaggcctgct ggacccaatt cggagactcc
    35401 aacaatacct ggggagccta ccagtgttat ggagatcaat ggtaccggct gaccgatcaa
    35461 aacgcaggtc cctctaccga ggtggattat tttacggaag aacagatcct ggtcgacctg
    35521 tacaatttta aaacaaaact agagggtaaa aaaataaagg accggcttgc cttgcaggaa
    35581 catttggata agatcgtgca aagagccctc aaggctggtt tgtatcaggc ccttgtcatt
    35641 gagaaggaag cggatattct ctccgaactg gacctggtat cgcccgccct tgagcgctat
    35701 aaatccctgc gcagcatcaa ccaggctgat ttttcagtca aagccctgga acaatattgc
    35761 aacctgcgta tgaaatcggc tttaggggat aaacgcatta ccgcctctca gattcgccaa
    35821 ttgatcaccg actttcatgc cctgggcatg ctgggcctta ccccggaacg attatgcctg
    35881 atgggcagcg cttatcagaa tctggccatc attgcccacc ggaatccatc taaaacaatg
    35941 ccatcggtga cagttagcct taaagaagcg gaaaaacagt ataaaaaagc ctttgttctt
    36001 tccgatcctg caaaggtcca ggatttgatt tatcctcttg caaactggct gatcctgaac
    36061 gcaatcaacg atgggccacc gaccattgaa atcaagggta agaaaatgaa tatatcctcc
    36121 tttctcaatc agacagaaat aaaactaaaa aaagaattgg gcaggaatct caatttctgg
    36181 caggatatca gcctggtcaa tctcggccag tgcaaattgt tttatacttc ttcccttgtg
    36241 gaattggatg ggataaaaaa tgaaattatt aataactata aaaacaactg gaatctttcc
    36301 ggcacgctga aacatttaca gacggaactg gatcagattg actttttgat ttttggagtg
    36361 gagcggatga agaaaagtaa aaggtggaaa gagcggatga aaaagaatct tgcggagatc
    36421 agggatgaat tgaaggaaat gtagccgcga tttcagtcgc caggaattat gggataaaaa
    36481 acctgctgcc gcgaattcag tcgcctggta tgtccaaagg aacacttact ttctgaaacg
    36541 gttgctttag gcgtcctcgt ctaaagcgta tttatttaaa ttcattttat aagccttgtc
    36601 ttttctataa cgcacatgga ttggtattga ggttaaataa ccgccattca actatgatat
    36661 tccggaaaaa ccctgttcct caacccattt aaccagtgca ttgccgaagc ccaaaaagaa
    36721 ttcttcgctc cggattttat ttcaccgggg acttccgcag acagaatctc cagcatcctt
    36781 tcccggcttt tcaccaggtc agatagttct ataaacatat ccgcattaat cgcttgtgca
    36841 tagggcagaa tgaaggcttt ggtctcttcc aggtattttc ggataaaacc ggtatcgtgt
    36901 acaaacccaa gggcaaaaag gttgctgatg cggtcggcca gcttcaggat tttagccctt
    36961 tgacttcccg acttcatgac tctttgcaga aattcggctt tgggctcctt ttccccgttc
    37021 agccggcgga tcgagacttc cataaccagg tcgtataccg caggaccgtc ttcatccagt
    37081 tgtcggatct ccccctccgt aacccccggc agatgggaac cgtcttcaaa tagatcgtgg
    37141 atcacggagg ccttaagcag gatcggatcg gtcattttat aatccagcag gatgcccagg
    37201 gtgctgatct ggtggcggaa catattgctt ccggacctgc gccggacccc gatcagggcg
    37261 gtagctttgg tgatgtaggg agcgaggatc acctttcgga gggaagattc aaaattgttc
    37321 atggttaaac tagggtggcc cggaccgggg tcattatttt aagcggagag agcagccgtt
    37381 tattttacaa attcctgcac cctttccacg atcagcaaat cgccgatttc attttttttg
    37441 tcataaaccc tttttaagag ggctctgttt tttcgtttgc tggtcagagt ggagtatttc
    37501 atgaccttgt aggagtccag gatccatgga tatttggtct tgagataact gatgacgttt
    37561 aatctttttt tactgtattt tttttcattc atgatcagga acagataatc aatgccgcgg
    37621 aatttgttga ggatggtatc cagggtggga ttatggtcaa ggtcttttct cagggtatcg
    37681 gatacgatga tttcactgtt tttatcaaaa accgaaatgg ccttggggct gagctcgtac
    37741 atcatttcaa tgccaccgaa agaaagcgtc gaataagttt cgagcttgaa gatccccggg
    37801 tcgaatcccc tgattcgtcg ccccgacagc gagccggatg cggtgaccgg gccgtgttga
    37861 ctgatcatca gataggattg gctgcggttt cgggcggcgc tgtcctggat attgtgggcg
    37921 gcatagatca caatatgact ccattccttg gtcccccgca ggttcatgac atatttatcc
    37981 cagcggtggt ttacgatctc aaaaggcgtg gcgagcagtt cgccaaatcc gcagtgtcca
    38041 tcggaaccaa aaacataaag cttcagcggg tattttttat actgcgtctc gtctttggca
    38101 tcggaacgat aactcgtatc cctgctcgcg ctgaaataaa atttataatt gcggagcttt
    38161 tgcatgggtt tggtcagttt ctgggagatg ccttcaaagg cttcgctgga ataattgact
    38221 acgtttatca gttcatttac ctccctggcc cgctccctgg gggtgcccgg ggcggcggag
    38281 gccacggtat tggcaatgga atccctcagg gccggatcca atttggtaaa attgcaggag
    38341 taccacaaca gatcccgttg aaaggcaggg gtttgtacag ccatcacccg ctgggcaaag
    38401 gattccgtcg cataaaagga aatcagaacg aaaaacagtc ttttcattta cccgttcatc
    38461 agaatgtaaa tataatataa cggataaaga atatttaatg tgtgggtgac agggaaaatc
    38521 attttttccc gctcaaattt tctatcccta aaagtcgtcc cccatctctt ccccgccaat
    38581 catctctatc ctggtcttct ttacttctga ttttgcttta cccctgacca cgcccatttg
    38641 tccgtccata atcctgaaca gcccaagacc gataatcccc tccttgccat tcaggtccag
    38701 tccaatggat ttgttgaagc tcatcgataa ggccgcatgg ccaaggtcaa gatcatttaa
    38761 tgctttggcc tggccgctga aaatcagttc cgtatttgat tccttggtgg aaacgaccac
    38821 cgcttttttg gcatggtaaa cctggtgcac cactttatac tcagcgcgcc agaaggcctg
    38881 gccagccagg gacatggcca agacattggg actggctatt tcctttactt taatgagggg
    38941 agattgaatt aaaaaggatc gggctcccga aaattgaact tttacctggg cgtttaccga
    39001 accggacggg atgttttgta cgggcacccc tgcatccagt ttgatcaggc gggttccctt
    39061 ggatttaaag tcgatatggg catccgggct ttccagggtt tcaaatgaga ttttaaaatc
    39121 tgttttaata ttgccccttc taacaaaaat gcctccgtcg atgaccccgt agtcacccag
    39181 ggagaataca ttaaccaccg gaagccaggc ggcctgtacc cggagttccc ggcggatgat
    39241 ccggctaaat tgttttgcta tacccatgta tttttgaatt tatcatgaag cagtgttcag
    39301 attatgtaaa tactttcatt taagttaccc tttattttta aaaatttggt caccatttac
    39361 tataagtcca gtctgccgct ctggctggcc cttcttatta gaagccaggc agacttccag
    39421 gtctcgttac ttcttaactg ataaactgat aaactgataa actggcaaac cgataaaccg
    39481 gaaagccatt ttcctcaaga aagatgtatt cacctcaaat caaacaacac gagcaatgaa
    39541 ctcccaatcg tcagccaaag aatcacctcc cggaacccct tttcattgag tttccggacc
    39601 aggaagaccc ccaggtaaaa tcctccggcg atcacgggga ccaggacaag attgagttta
    39661 aaggtggaag gggtcaccgt cttccaaacc cagatatgaa agggcagttt aaaaagattc
    39721 agcagaaaaa acatccaggc cgccgtaccg ataaaactgt ttttgagcac ccgcatgctg
    39781 ataaaataca cattggaaaa ccctccggcc aggttgccga tcatggaagt aaagccggta
    39841 agcaggccca tgctgccggc aaataaccaa tgatccggca cggaagccag gggccttttt
    39901 tcataataaa tcaaggcggc cagggaaacc aggatgatgg ccgccatcaa ttgtttgaag
    39961 acccggtcat tgatcaggtg gccgaatacc gcggcaaaga ccacccctgc agcaatccag
    40021 ggtaataatt tgaccaggtg ctcccagagc gcatggcgtt tataatgttt tacggccagg
    40081 atgtcggcaa atatcagcat gggcagcacc attcccgtcg aagccctgct gccaaatatc
    40141 caggcgagga tggataccgt gatgatggat atggatccga taccggcctt ggacattcct
    40201 ataatagcgg cagcgagaat cagcaggacc cactggaggg ggctcaggga ataagcgggc
    40261 aggagggaac cggggaagga catgggaggg gaaagatacg atataggccg aagctaagct
    40321 tcgtccaaac ctgcaatctg tgttgtaatt gggggccggc tcccttcagg gatggggcgt
    40381 gcggatgaca acgttatatt ataaaaagcc gaagccaagc ttcgtctaaa ccggtgatgg
    40441 tgtgtcgctg tgggaactaa gggatgggcc gaagccaagc ttcgcccaaa caagatgttg
    40501 tgtcgttgtg gggctataag ggtgggccga agccaagctt cgtccaaaca agacgttgtg
    40561 tcgttgtggg gctatggggt gggccgaaac ctagtttcgt ctaaacaaat ggcgttgtgt
    40621 cgttgcggga gttatagggt aggccgaagc taagcttcct ttagacttgc aatatggtat
    40681 tttagaacat attaccattt ttattaatgc atttcttttt tgtta
//
LOCUS       DAOQXT010000035        40286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1131_length_40286_cov_7.100047, whole genome shotgun sequence.
ACCESSION   DAOQXT010000035 DAOQXT010000000
VERSION     DAOQXT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 40286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40286
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1131_length_40286_cov_7.100047"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PKM27_08060"
     CDS             <1..778
                     /locus_tag="PKM27_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR07253.1"
                     /translation="SLSFYAVLYLIGPFGGGGLQGRGGSSQEDALKAALNRVEEIRSF
                     LREAKAYSKSGGPVNLKYEAVKGLFDKSQKLFVHCDLTKEILMALDFKKEFDLDLVIE
                     GGTDSWMVADILREHQVPVILSEPHNLPSMADDAVDQPYKTAYLLHKAGVPFTIAIDG
                     FWQQRNLPFQAGTLAAYGLSKEEALSAITLQAARILGIDRQTGSLEAGKDANIVVSDG
                     DILDMKSSKVTHAFIQGRQINLDNKHNQLFEKYKYRYNLK"
     gene            complement(785..1558)
                     /locus_tag="PKM27_08065"
     CDS             complement(785..1558)
                     /locus_tag="PKM27_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNR07254.1"
                     /translation="MRVVLAWSRMIILVLVIFLFLMAYLLTTVFLGQDIRRAFRFRKA
                     GVRVMGWVLGYQIRAFGKFDQIQPAIYISNHRCFSDPVIALHYYHFFPIGKAEIEKYP
                     LIGLAAEKTGILFVQREDRTSRNSVKEGMRQKLRQGFNIFLCPEATTSVLQLTKDFKK
                     GAFEVAVEEKVPVVPLAILYHKPELDFWIPGDSLLTHFIKQFGKWRTRVDIWFPDHAF
                     RGNDPATLLQDCKGWIDSKLSAIPVPQWVIDRSVDTLKV"
     gene            complement(1602..2888)
                     /locus_tag="PKM27_08070"
     CDS             complement(1602..2888)
                     /locus_tag="PKM27_08070"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HNR07255.1"
                     /translation="MAEVIRMPRMSDTMEEGNILAWHKKVGDDVKVGDVLAEVETDKA
                     TMDLESFYDGTLLYIGVEKGTIAVNAILAVIGKKGEDYKSALEAAGAPAAGQTPAADA
                     SSNPQPVTEAAPAPLAAPPVAAPPVAADSHIKASPLAKAMAKEEGIDLQKMTGSGDLG
                     RIIKRDVEAALQQKTSPQTAPAKPAAAAPIAPAPVFNISPGGAAYEDKPLSQMRKTIA
                     RRLAESKFSAPHFYMTMAIDMSKAVSARAAMNAVAPSKLSFNDLVVKAVAMALRKHPV
                     VNSSWTGDAIRQNHQIHIGVAVAVAEGLLVPVIRYADIKTLTQINAEVQYLAQRAKER
                     KLQPEEMQGNTFTISNLGMFGVDEFTAIINPPDSCILAVGGITEQAVVREGKIEIGHI
                     MKVTLSCDHRVVDGASGAQFLQTLKGLLEEPVRMLV"
     gene            3027..3644
                     /gene="recR"
                     /locus_tag="PKM27_08075"
     CDS             3027..3644
                     /gene="recR"
                     /locus_tag="PKM27_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNR07256.1"
                     /translation="MKFSSKLLEEAMMAFHSLPGVGKKSALRMALHMMKKDPEYARKF
                     SEAVWNARNKIRTCLRCCSYSDEELCSICADPRRDEHTVCIVESTRDVMAIEETNQYH
                     GVYHVLGGVLSPIDGIGPAQLNIEALTRRLASEEIREMIMAINPTIEGETTIYYLSRQ
                     VQPFNIPVTVIARGVSFGGELEYADEMTLGRSILARTPYQIKSVT"
     gene            3647..5521
                     /locus_tag="PKM27_08080"
     CDS             3647..5521
                     /locus_tag="PKM27_08080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR07257.1"
                     /translation="MDLTVIILSYNVRYLLESCLESVETAMRRIQGEILVVDNASSDE
                     SVEMVRSRFPEVRILANDQNIGFAKANNLAIKQARGKYVLILNPDTLVHEGALRACLD
                     YLESEADVAAAGVRMTDHAGRFLRESKRGFPGLWPSIWKLSGFSRLMPGHAKINHYYL
                     GHLDEAGIHPVEVLTGAFMMVKKTVLEEVGGFDERFFMYGEDIDLSFRIRKLGYRLIY
                     LGGQSIVHLKGRSSALHSAAYVLNFYRAMSVFVKKYYVNPALKTIVMTGIGLAAVLSW
                     LKRKIVRHFLPLMDILLLSLLLFLVQYLWARGWFEDPAYFHNSVFLLNALSYVFLWFM
                     ALVLFEAYRPYQEKPAWLALKAVLAGTLVILVLYSLLPENWRSSRAVILMSGLSAALV
                     LPWSRNLLRPGLAALRALYTGDQAGEKSLNPLFKRLCHDHYFSYLSREDLSGLREPET
                     RVAKLKDQVQAQDITHVILESPAKSKEDLLLFTRGLKGRVKFLVPEKNGWPETGVMAS
                     IPGLKEVDIRLGKPMYRTMKWLFNFVLTVLILPWGWLRRDIRKNYFALLLGTKQWISY
                     HQPPDPRLPSQRPGLWHPGFDPVTGDDYPWQDLNFEYAGKYTPGRDILIVLARLFNFN
                     "
     gene            5521..6723
                     /locus_tag="PKM27_08085"
     CDS             5521..6723
                     /locus_tag="PKM27_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNR07258.1"
                     /translation="MNEMLSPEKITGMSKQYLPETIRLRRYLHEHPELSFQEHQTAAF
                     IENTLRAYGLEPARMANTGVIVLIEGQKPGGKVVALRADTDALPIQEISSEHYRSRNP
                     GVMHACGHDVHTASLITVARILHETRPAWGGKVKIIFQPGEEKFPGGASLLIGEGVLQ
                     NPVPEIILAQHVFPELEAGKLGFRQGPFMASADEIYITVHGKSGHGAIPHSTRDPILI
                     ASHIVVALQQIVSRNNNPLTPSVLTIGKIQSTGGATNIIPGEVKMEGTFRTFDEAWRK
                     EAKSLIRKITEGTAQAMGGQVEIDIKDGYPVLLNDPKLTHRMQQWAIQLLGPEQVVDL
                     PMRTTAEDFAYYSQLMPACFYRLGTAHPGRPGEKRVHTPDFDIDEEALATGVAFMAWA
                     AVSWLKTA"
     gene            6828..8102
                     /locus_tag="PKM27_08090"
     CDS             6828..8102
                     /locus_tag="PKM27_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNR07259.1"
                     /translation="MAKILIADDERSIRRTLREILEFEKFTVEEASDGLECLSKIKQQ
                     DFDVVLLDIKMPNLDGLETLEKIQQLNPDLPVVMISGHANIDTAVDAVKKGAFDFISK
                     PPDLNRMLITIRNAMDKTCLITEKKVLQHKISKSRIQEIVGDSPAIAKVKETIERVGP
                     TEARVLITGENGTGKELVARWIHEYSGRKQGPIIEVNCAAIPSELIESELFGHEKGSF
                     TSAIKQRLGKFELAHGGTLFLDEIGDMSLSAQAKVLRALQESRITRVGGDREIKVDVR
                     VVAATNKDMRREIEAGRFREDLYHRLAVIIIEVPSLNQRSEDIPVLVEHFNQLICQEY
                     GIPEKGFTDKAIKALQKVQWSGNIRELRNVVERLIILSTGRITDKEVYNYVVPTALHG
                     SRYREIFDRFQDVRDLRVFIEREYEQYKAESV"
     gene            8139..8843
                     /locus_tag="PKM27_08095"
     CDS             8139..8843
                     /locus_tag="PKM27_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNR07260.1"
                     /translation="MTRFQREMKKWTGLRGENSWTLFKVMAEMVDGFEVLNRVGPCIS
                     IFGSARTRPGNFYFEKASEIAHRLTDEGYGIITGGGPGIMEAGNKGAFDAKGTSVGLS
                     IDLPFEQTYNEFVDPDKILKHRYFFIRKVMFVKYAQGFVVMPGGFGTLDEFFEVLTLV
                     QTKKINRVPIILFGVNYWEGLRKWLVETVDQEAHNINPEDMDLFLITDDVESVVQHIN
                     AFYDARDDHFRPNYEL"
     gene            complement(8899..10803)
                     /locus_tag="PKM27_08100"
     CDS             complement(8899..10803)
                     /locus_tag="PKM27_08100"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07261.1"
                     /translation="MSLVKRLLFLCLLGWMGLPSALEAQRNLPSNFPRSVEEQVPDSI
                     EQEELTDEPPDTVRIYTPYRLKTAVFFDDTLLDPLFHHYDAIRLQGIPYFHLGNNGSP
                     HRPAGYVLDKRQGLDLGLRSFDLYRKDYEDLLFLDSRRPFAIFRFAQKSFSQEDLQLE
                     SRFSKTFQNGLHLDLNYSSIQYKGEFTEQITKDRTGAFSLWYHSPKGNYDAIVSYLTN
                     DIRNQDNGGVPFADSLLTISQYRRATETLDVLLDGRSRQRNNEFSVQNGFNLKWKGNI
                     LGAMHRIALRKETIFYEDFTSPNDSFYYGPFYVRDTQQLDLRAGGLVNEFRLDGLLAG
                     KNYFMGGLKHEYYTIDRGPAEFRRNLLSALGQYQQSLFGTLQLNAEGQWGLLDNLGEY
                     YLKGQVQAASRKLGSLEGHLILQRSPVPLAYAEMYNRNTLVFSNDFEKPLTNQIGGSI
                     RVAALSFRAGIDQSILSNTIYLDSLRRPVQEGSALSVTTLYFDKAFDFGKMHSMHAFT
                     YQISSAKEILRIPTWHTRHQLYYKGSWFKNAMGVLTGVETRIIPGFRSTAYFPLLASF
                     YNADRAPVDLHPYLEYFFNFQLRNFRGFFKIEGLEYLFYTAGKTFYETYDQPLFRTNI
                     RMGFTWTLRD"
     gene            complement(10808..11629)
                     /locus_tag="PKM27_08105"
     CDS             complement(10808..11629)
                     /locus_tag="PKM27_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR07262.1"
                     /translation="MSNQLLKNKTGIVFGALDEQSIAWKVAERAVEEGARITLSNAPV
                     ALRFGKINELASKLQSEIIPADATSMEDLTTVFTRSKEILGGPVDFVLHSIGMSLNVR
                     KGKHYTDLNHEWMLKTMDISALSFHKMMQVALKTDGIAEWGSVLGLTYIAAQRVFPDY
                     NEMADAKALLESIGRSFGYHLGTLKKIRVNTISQSPTWTTAGSGVKGFREFFDYAQKM
                     SPLGNATAEDCANYCVTLFSDLTRMVTMQNLFHDGGFSSMGISPLFFAEEAPSEK"
     gene            complement(11643..13301)
                     /gene="recN"
                     /locus_tag="PKM27_08110"
     CDS             complement(11643..13301)
                     /gene="recN"
                     /locus_tag="PKM27_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNR07263.1"
                     /translation="MIRRLKIQYFAIIEDVELEFSPNLIIITGETGAGKSILIGALNL
                     VLGQRADIRAIGNQTAKCVVEAEFMIKGEELKPFFEAQDLDFDHEIVIRREISPQGKS
                     RAFINDTPVTLEILQTLSAALIDVHQQFDTLDIYQVSTQLRMIDALAGNKALLEEYQQ
                     EYKRYTTNLETLRALKDRLTREEQEAEFIKFQWKELDELNLVAGEWDTLETEQKALAH
                     AGEIKTASENIHLALTEDERSLTGMLSDLDRSLHPLGSVHRGLQSISDRIHSILAELE
                     DISAECKNISEQIDLDPGRLAEVEERLSSLFRLCQKHKVKTVEELIVLHQQFSSRLHM
                     ASDLGEQLAALEKEIKKQETSLLGKARELHARREKVIPGFIEKIYVLLRPLAMENTQV
                     RIELSPLDSLNQTGLDQIDFLLATNKGGAFLPIKQIASGGELARFNLVTKSLVAQAIP
                     LPTLIFDEIDIGIGGDVALKMGRILKDLSVHHQVICITHSPQIAAKGDLHYFVYKDES
                     AERTTARVKKLNKAERVQTIAAMLSTNPPTAAAIKNAKELIEIV"
     gene            13425..14447
                     /gene="lpxD"
                     /locus_tag="PKM27_08115"
     CDS             13425..14447
                     /gene="lpxD"
                     /locus_tag="PKM27_08115"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNR07264.1"
                     /translation="MKTSARELAEFLHATIDGDPDIEVTHPSKIEEAGPGSISFIGNP
                     KYESFAYTTRASILVVPNEFKAQRPLQATLLRVDNVYQSLGNLLSRFENNGHPVGISD
                     QASIDETVSLGAGVAVGPLSVIEKNAVIGAGSLIYAQVYIGEQVTIGRDCTIYPGVKI
                     YAGCKVGDRCVIHSNTVIGSDGFGFVPNPEGRMEKIPQLGNVELGDEVEIGSGCTIDR
                     ATMGSTRIGKGCKLDNLIQIAHNVEIGEHTVIAAQAGIAGSTKIGARCMIGGQVGIAG
                     HLRIPDGTQIQAQSGIMSAPSEPNQKLFGYPAISYHDYLRAYALFRKLPDLEQRLRDL
                     EKKSGE"
     gene            14606..16006
                     /locus_tag="PKM27_08120"
     CDS             14606..16006
                     /locus_tag="PKM27_08120"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNR07265.1"
                     /translation="MKQCTIARDAVFGGIGLHTGKSATMTIRPAPVNHGIRFQRIDLK
                     DSPILPADVNRVISTNRGTTIKSGDAQVSTVEHILSALAGMGIDNVLIEIDGPEVPIM
                     DGSSKPFTDAFIQAGITVQELEKDYLELTETVAFKDELSGTELIAVPSDKFEVTAMID
                     FRSPFLGQQYASLNDLSEYEKEIAPCRTFVFLREIEKLANENLIKGGDLNNAIVIVDR
                     MMTQPELDDLAQKLNKPSIQVEKEGILNTVELQFSNEAARHKVLDIIGDLALVGKPIK
                     AKILANKPGHTANVEFAKLLKKKYNENRKLKGKPNYNPDETPLMDTERIQKLLPHRHP
                     FLLIDKIIELSDQHVVGVKNITFTEACFAGHFPGNPVFPGVLQIEALAQTGGILAISQ
                     QPDPGNWDTYFIKIENTKFKNKVMPGDTLILKMELMSPIRRGIVQMLGTAYVGNKIVS
                     EGELTAQIVRRESNPT"
     gene            16009..16824
                     /gene="lpxA"
                     /locus_tag="PKM27_08125"
     CDS             16009..16824
                     /gene="lpxA"
                     /locus_tag="PKM27_08125"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNR07266.1"
                     /translation="MDIVTQYQLNVVHPQARIGKNVHISPFVTIEGDVEIGDNCWIGP
                     NACILDGTRLGENCRVFPGAVVGAVPQDLKYSGEQSILEIGNNVTIREYCTLNRGTKA
                     NWTTTIKDNCLLMAYVHVAHDCVLEENVILANNVNLAGHIHVGKYAVLGGLSAVHQFV
                     KIGDHSMIGGGSLVRKDVPPFVKAAREPISYAGINAVGLKRRGFSTDSIKIIQDSYRI
                     LFVKGYNTSQALEIIEKQIPDSPERARILEFVKTSERGIMKGFRQINGNGSKD"
     gene            16808..17425
                     /locus_tag="PKM27_08130"
     CDS             16808..17425
                     /locus_tag="PKM27_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR07267.1"
                     /translation="MEVRIDRLSKRYQHGWVFRDLTHDFRNHQCYGIAGRNGSGKSTF
                     LKIISGLLSATRGEVNYHLGGQTVSRENIYQEVNVAAPYTDLIEEFTLREMVRFHCRF
                     KPLSVDRAEDWIEMTGLSRAADRPLAFYSSGMKQKVKLALAMYSRGSLLLLDEPTSNL
                     DDNARSWFFDHLEKQKGNKTVFIASNEAEDFRMCEEVIRLDATTP"
     gene            complement(17379..17741)
                     /locus_tag="PKM27_08135"
     CDS             complement(17379..17741)
                     /locus_tag="PKM27_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNR07268.1"
                     /translation="MLPALKDEKLYYSIGEIADMFGVSKSLIRYWESEFSILKPHKNS
                     KGDRRFTKENIDQLQLIFDLVKNRGFTIEGAKNEIRQNKDLLKQKQAVLQKLIHLKNE
                     MLRFKESLRPAESLPHTS"
     gene            complement(17928..19370)
                     /gene="gatB"
                     /locus_tag="PKM27_08140"
     CDS             complement(17928..19370)
                     /gene="gatB"
                     /locus_tag="PKM27_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNR07269.1"
                     /translation="MTNSTSSWETIIGLEIHIQLNTLTKAFCDDPARFSTEANTQTGY
                     VSLAHPGTLPLPNGKQVESAVRLGLALDCTINTHSWFERKQYFYPDLPKGYQISQQAS
                     PVCLGGRMDLETSQGSKTIRIHHIHMEEDAGKSVHDTDPVWSRIDLNRAGVPLLEMVT
                     EPDFRTAEEVTVFLTDLQRLVRYLDISDANMEEGSMRCDVNVSVRPSGSEEYRERCEV
                     KNINSRRFARKAIEFESARQIALWEAGQAVVRQTMQFVPEEGVTVPIRQKESAHDYRY
                     FPDPDLPPIKISQAQLDTWRNSIPTLPSEYLRLFTREKNLSLADAQVLAEDPEVARYY
                     VELVQEEPALNKSAANWLIQTMLPWLREQNKKIGELPFGVKSLGFLLSQVRLGVLNLQ
                     QAQEVWIQITTSSQASLEDLVKEKLDAARQFDIPGVVEDLFSSFPVEADEFKKGKKKL
                     LGFFLGEIKKKFPGIPVKEVQEALNRYLAG"
     gene            complement(19388..20395)
                     /locus_tag="PKM27_08145"
     CDS             complement(19388..20395)
                     /locus_tag="PKM27_08145"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HNR07270.1"
                     /translation="MVCPNCNYRETTDAKYCSSCGQTFGADKPTVREFLSDFFDHVLS
                     LDSSFFRTLKKIWIPGQLTIDYFNGLRKRYYHPLRFFFFLLVIHLGVLSGLIPYDRIF
                     DEVNNQTNDILTRHKIRTELARHAESGQTRVSRMAIDSLMSWFNIDTSNAPGPDSIGT
                     RRISVLGFQNLDPEDVVLLPIDSITAKYDISSFWTTLMVGQAKKFMLDPKGMFRFIIG
                     NFSWMMIVMIPILALFMKVQFLRGKRYLIEHLFFLYHWHAFSFLIVSLLVLLFRDFIE
                     FFWLPVMVTVILYGLIAWIRYYRQGIIRSVFKFFTLGLAYLVLFSFFIFFTALISFGF
                     Y"
     gene            complement(20437..21426)
                     /locus_tag="PKM27_08150"
     CDS             complement(20437..21426)
                     /locus_tag="PKM27_08150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNR07271.1"
                     /translation="MAGKPTKYVYNHHTLQFEKAVEPLKIKLFRVFSFLSTALVSGLI
                     VFFVFHKYLPSPNEKLLMKELESVKDHYNIMSDQLDLMSKALANIQRRDADVHRQLVG
                     MDPIDEAIWDGGKGGHDLYENFRSLPSSGELVGKTAEKMERLERQLVLQSKSLDKLQD
                     VAAHKEEMLASIPSIKPVRIDKLNKSLRFLSGFGKRLHPVHKIVKMHTGIDFSAPKGT
                     PIQVTGNGRVIDIETNYSGYGKCVKVDHGYGYITLYAHMDRIDVKVGQELKKGNQIGV
                     IGSTGTSTAPHCHYEVRYKGTPINPINFCLDNLTPSEYQELVKMSEQQNQSLD"
     gene            21725..24346
                     /gene="alaS"
                     /locus_tag="PKM27_08155"
     CDS             21725..24346
                     /gene="alaS"
                     /locus_tag="PKM27_08155"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNR07272.1"
                     /translation="MTSSEIRRYFLDFFRSKQHFIEESAPIVLKNDPSLLFTNAGMNQ
                     FKDLFLGNKPVVHPRIADTQKCLRVSGKHNDLEEVGLDGTHHTMFEMLGNWSFGDYFK
                     EEAIDWAWELLTQVYRMDPERLYASIFTGDENEKLVRDTEAGAFWKKHLPEDRIILGS
                     KKDNFWEMGDTGPCGPCSEIHVDLRTEEERNAIPGRDLVNTGHPRVVEIWNLVFIQFN
                     RKSDGQLEELPAKHVDTGMGFERLCMVLQNKTATYDTDIFRGLIAHVEKATGIAYRGS
                     YDPTAKSDIAMRVLADHIRAVAFTIADGELPGNTGAGYVIRRILRRAVRYYYSFLNLR
                     QPFFYTLVPILAGYFKEVFPGLDKQEGFVSKVIEEEEKAFLRTLEGGLRRLESLSTGS
                     EILDGSAAFELYDTFGFPIDLTRLISAERNMRVDESGFQEELEKQRARSRAAAVTETG
                     DWVVLRDQKETRFIGYDQLEAEGVKIIKYRTVKSKDRAEHQLVLDKTPFYPEGGGQIG
                     DTGIIQTANEIIRVLNTKKEIDLPLHIVDQLPSDPRALFFAAVDGPRRKRIQNNHSAT
                     HLLHAALRKVLGTHVHQKGSLVAEDYLRFDFSHFQKVSTEELTRIEHLVNQKIRENIA
                     VLEQRDLPIRQAQEAGAMMLFGEKYGERVRMITFDPEYSRELCGGCHVSATGNIGLFR
                     ITGESAVAAGVRRIEAITGDAVERYIEEQKQLMDRIREALKNPKDVLHALSHLVDENK
                     NLRSQLAVYQEKEIANLKQKLKAEALLRDGVHYVTGLLENLDEKDAKTLSFQLEKELS
                     PAFILFGFVSGGKPMLMLHLSRELVENKGMNAVHLIKRLAVHIEGGGGGQAFFATAGG
                     KNPDGLTSALVEALQSV"
     gene            24358..25362
                     /locus_tag="PKM27_08160"
     CDS             24358..25362
                     /locus_tag="PKM27_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNR07273.1"
                     /translation="MDKRFLVCCATLLLMGLSFEMAAQASLSRIRVESNRFVNEQNET
                     LVFRGYNTSDPDKLVYDGQWNKSYFEEIRAWGGNIVRFPVHPAAWRRQGKNNYLRLLD
                     QGIRWAGEAGLYVIIDWHSIGNLKSGLFQNDSYETSLKETFEFWRAMATRYKENPVVA
                     FFELYNEPTVFNGQLGVCSWSEWKAINEEMITIIRAHGCKAIPLVAGFNWAYDLTVIK
                     DEPINAPGIAYVSHPYPQKREKPWEAKWTADWGFATEKYPLILTEIGFSGPEEAGAHI
                     PVISDESYGEAIVKYCQERGISFVAWVFDANWSPRMFSDWKFTPTRQGKFFKEALTRK
                     "
     gene            25529..26032
                     /locus_tag="PKM27_08165"
     CDS             25529..26032
                     /locus_tag="PKM27_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07274.1"
                     /translation="MKTKMRRTWLLAGLVLGSCQVELDNFFGKQNPGTYPNVDEALRP
                     YFQRFEAEAAARGLTVDLADAYITGYISDIPENQVIGQCSYSSNDPHRVTIDKPFWNQ
                     ASDLEREFVVFHELGHCYLARVHDESMDARGICLSIMRSGTGACRDVYNAASRSGLIQ
                     ELFTEKD"
     gene            complement(26037..27215)
                     /locus_tag="PKM27_08170"
     CDS             complement(26037..27215)
                     /locus_tag="PKM27_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNR07275.1"
                     /translation="MKYLLLAVPFFLAYCSSGPLPDARVTEILDSLRYKYAPDKRTAI
                     FDIDPVFQGKTLILKGEVDNGRAKEELVRRLEGIAFVDSIMVLPSPSLEGYHFGVVTI
                     PLANIRSRPGHSSELVTQALGGTVLRLYKYSNSWLYAQTPDDYLGWVDEDAVHLMDSA
                     AYKAWIARQKIIIIADQTYLFSAPSLSSERLSPLTSGNILKLEDTRGDFIQASFPDGR
                     NGYIPSSHTTWLEEWKFQANTLPAGEDLVRTAMGYLGRPYLWGGTSGNGMDCSGFTKT
                     VLFQHGWQLPRDASQQVKVGAPVETDTTLKNLRPGDFLFFGRKATATDPERVTHVAIY
                     AGEGKIIHASGYVRVESLVRGDPSFTESRLNSLLGATRPLSAPKEHGIYPLSDIPYYD
                     "
     gene            complement(27318..28022)
                     /locus_tag="PKM27_08175"
     CDS             complement(27318..28022)
                     /locus_tag="PKM27_08175"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNR07276.1"
                     /translation="MKNIILLLILVVAGIQTASSQKIKGTGPKVTRTLELASFEKIES
                     NISADVEITQGATQQITVEGQANIIDLIQTEVNDKKWKIKFSKNTWGDYEDLKIKITL
                     PKLTAVGLGGSGSIVTTNMFTADDFNVGVSGSGTVKVMVEADHVDCGLSGSGNIHLKG
                     KTRDLKIGTSGSGNVRALDFPASQVKIGISGSGSCEVSASDMLEAGISGSGSVRYKGQ
                     PKIKTGISGSGSIESI"
     gene            complement(28173..28424)
                     /locus_tag="PKM27_08180"
     CDS             complement(28173..28424)
                     /locus_tag="PKM27_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07277.1"
                     /translation="MKISIDLSLYPLDKNYSGPIRNLLSRLSQYRNVEVRNTPMSTQI
                     TGDFDACMHLLQTELKHSFSQGQTMVSVIKMVNLDMEDN"
     gene            complement(28455..29615)
                     /locus_tag="PKM27_08185"
     CDS             complement(28455..29615)
                     /locus_tag="PKM27_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR07278.1"
                     /translation="MFSHLLRFLLHRQSVAVGAVFLVMGTLFGNWATLIPIVKSKFGL
                     DDAQLGLIILCMPVGAALANPASSWFIHRYGMQKMTLTSMVAMSLCYLLPVLSPVAWG
                     VGLGLFVTGIMIAFTNVSMNTCASIIEHRSKLHIMSTCHGLFSLGLMLGSLMASTLIG
                     LKTPPLVQSMIISGLMLLIWLMVRREILQIEEELTAEEEPVKPKFTFPRGPLLIMIGI
                     ALCTNLTEGSMADWTAVYLRDVVHAPEYLIGWGLASYSFFMAMGRFSGDYFIPRYGAR
                     SILKYGGWISALAIAVAVFFPKTWICIAAFGMVGLGVSCGAPILYGASTRLPNLAKGV
                     GLATLNTFSVATFLGGPVIIGFISKAINLQSALFIVCLAGLAWVYLSTRLKW"
     gene            complement(29743..32235)
                     /locus_tag="PKM27_08190"
     CDS             complement(29743..32235)
                     /locus_tag="PKM27_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNR07279.1"
                     /translation="MKQLSWFLGPKGENEEIFTNTILTIIRDYIHWRRNYFPADPILI
                     TRRIQRQHEEEFDKLMQGVAEMTSQLRRNFPFYSPRYIGHMLSDINMPSVFGYFAAML
                     YNANNVTPEGAPVTVEWEIEASNQVLKMLGFNTPPVPPGKEAGIKEWQAYEEKLKTEF
                     GWAHITSGGTLANVEALWVARIIKYFPLTVHDIAREKGLDIAVNLPNGKRADLKALSP
                     REAIGLSPETSIGLFACLIEAISKSEKINIAEAVKKSDLYLRESPYSLTNHLGRIFSE
                     FPPVIFASGAAHYSIKKAADVLGIGRNNVVLVKTDSRFRLDVKDLELKIMTALEEGKI
                     PIAVIAIAGNTEEGAVDPVHEIAALREQIEARMNTSFWLHIDGAWGGYIRSLFKLSQE
                     EKLEILVKKLNRLLDPEAGPDTDNGLVSEIYQLLETTRSMIQATPDDEAEPLLISVEQ
                     RILRLKEKAGRNEFNGFRKKYLALIDEFGTILRSPYGQPLSEIIGPEDFKIRAEDRAR
                     DVASHVSDTIDFSLKAQTKPIRISWGGDLQVVKAFLSFKKADSITVDPHKLGYIQYPC
                     GLVAFKNDRIRHFIMQRAPYITSTEHDILVHHPPRHVKDVDYSKLNSNNIPYENYHIG
                     IDAFAPFILEGSKPGAAAAALWFSSRMIPLNRSGHGRLIKSSLLATRELYEWLRHWEE
                     IYQTARFPEPLTYTILTFPEPPDTNVLVFAVKNKTDQSLREMNALTGAVYGHFSIQVE
                     QGDKEHSYAQSFFVSHTRMDEQYYPFDSLADFFTNAGISRAREDYNAEGLLVIRCTPM
                     NPYTTAIRQSTSQNLIQEFVWQLHKVVSRLKV"
     assembly_gap    32340..32439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(32488..33840)
                     /locus_tag="PKM27_08195"
     CDS             complement(32488..33840)
                     /locus_tag="PKM27_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNR07280.1"
                     /translation="MEKKILLTEKEIPSHWYNIIASMPNKPLPPLHPGTRQPIGPEML
                     EPLFPMELIKQEVTGEKYVEIPDAVREAYKLYRPTPLYRAYGLEAALDTPAHIYYKYE
                     GVSPSGSHKPNTAIPQAYYNKQQGVKRIVTETGAGQWGSALSFACNMFGLECEVYMVK
                     VSYNQKPYRRIMMETFGAKVYASPSDHTESGRKILAADPHSPGSLGIAISEAIERAAP
                     DPDTKYALGSVLNHVLLHQTVVGQEALIQMEKAGELPDIIVAPFGGGSNFAGLSFPFL
                     RLNLEEGKKIRAIASEPLSCPKLTRGVFSYDFGDTIGMTPLLPMYTLGHEFVPASIHA
                     GGLRYHGAGVIVSQLLKDKLIEARAHAQLECFEAGVKFARAEGIIPAPEATHALATVF
                     QEAAKAREEGTRKTILFNLCGHGHFDMQAYGDYFAGKLEDHELTDAEISDLTSKLIAV
                     "
     gene            complement(33852..35210)
                     /locus_tag="PKM27_08200"
     CDS             complement(33852..35210)
                     /locus_tag="PKM27_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNR07281.1"
                     /translation="MPSLYSNLFYDRAFEFFEVRPVLEALLGFEKALARVQSRQGLIP
                     PPAAGAIEILCSYESINRERLMEESARSGNILIPLLGQCRQLLAGHPSEAAGYLHFLA
                     TSQDAIDTAAMIQYKKATAWINLNLRPLITGLVEIAEMHRHTPMAGRTFMQHARPITF
                     GFKVAGWIDGLLMVSEGLRFLKFPLQLGGSVGTAPGYSVKQYEEVSSGLSRELGLDLP
                     VKPWHNQRQYIHTMAHQLGALQGHLNKMATDLSLLAQNEVGEISFDGKDKGHSSIMPH
                     KTNPVNAILILANGLRIQAIAYSLSNTLTQDHERGSGPWHAEWESMEDLFRLVAGSLR
                     LSIEMIGEIRVNKERMLKNIDDGGGLVFAEHVSRMLSLKLNTEEAQALVRSFSTRVRS
                     TGESLQALLLKEESLRHLFSEKEISSWFEIENNLGLCDYFTGKMINRAKDLLSRKFKT
                     KS"
     gene            35393..36805
                     /locus_tag="PKM27_08205"
     CDS             35393..36805
                     /locus_tag="PKM27_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07282.1"
                     /translation="MEVFKNSTGAFAPQNSSTMRYCKDFILCALALLCMVNLAGAQKF
                     WLTTYEFPGGPKTGISLVQDSVLFVSTLNYVMRSFNEGNKFDTTLRAESLSTVFASRT
                     GTVFAGGKGKVYFTRDFGTSWDSVLVNTAAHITGFVENTARDPFAMTRIYDEEKGYIG
                     DGVLFSNTGGASWAFRNSGLGSQKACEHLVIDKNNRLYLAIGDEARTGMGGLFVSENN
                     GMSWQGIALPFDGKNHVPDQVIPTTITGLSVSPQDSLYLSLSGVAVNVGVSLNIYKSI
                     DDVTKNTFWNIMNVIPDNQFTDRNLNSIHFAGNGDWYSSFSGTLNVGGTYYSKNRGVS
                     WQRIDFGLGLDIFGMRNVQYFVEKQNGKIFMVHYLNELVHYTDTSLTTATGDVRIRNN
                     KLTVFPNPVASGENIKIELPASMQKCSIAVLNSSGQVVLAKRVNQSSRVEVDMTVVPG
                     VYFIRAIEGNVAAVQKVLVF"
     gene            complement(38276..39481)
                     /locus_tag="PKM27_08210"
     CDS             complement(38276..39481)
                     /locus_tag="PKM27_08210"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNR07283.1"
                     /translation="MDQAYLVDYVRSPIGNFGGGLSTLRTDDLAAWVIKKLMEKNPDL
                     PPGQIDDVILGCTNQAGEDNRNVARMALLLAGLPFTVPGETVNRLCASGMAATINAGR
                     AIQCGEAHLIIAGGVENMTRGPWVISKTSKAFGTDAQMHDSSFGWRFVNPRMKQMYGT
                     DSMGITAENLAAKYRIYREEQDLFACGSQEKAAKAVASGRMEKEIIPIEITDPKQNVS
                     LFSRDEFIKPATSPEGLARLKPAFKPEGTVTAGNSSGLNDGAAALLVASADAIVQYGL
                     KPLARIAGSAVVGVEPRIMGIGPVPATIRVLEKTKLKLDDMDLIEINEAFAVQVLACL
                     RELGLDPGDPRINVNGGALALGHPLGMTGARILGSAALDLHLLHKKYALVTMCVGVGQ
                     GYAVLLEKV"
     gene            complement(39481..40182)
                     /locus_tag="PKM27_08215"
     CDS             complement(39481..40182)
                     /locus_tag="PKM27_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR07284.1"
                     /translation="MRLIYFLLVLPLIYSCTASRGTTGKKSIPLFNGKDLTGWHIDVP
                     EMDKNPNAINPFVVRNGYLVSLGTPGGHLITDRSFSDYRVEIEYRFAGKPGNCGALVH
                     ASKPRRLYGMFPQSVEVQMMHENAGDFWCIGEDITVPDMEARRGPKENWGIDGDKNRR
                     IPNLTDGSEKPPGQWNSMNIECLKNEIKVWVNGDLVNHGFNATADHGSFALQAEGSEV
                     EFRKLLLTPIKKLSK"
BASE COUNT         9614 a        10053 c        10870 g         9649 t
ORIGIN      
        1 ctctctgagt ttttatgctg tcctttacct gatcggtcct ttcggcggcg ggggcctgca
       61 gggtagaggc ggatcttcgc aggaagacgc cctgaaggct gccctcaacc gggtggaaga
      121 gatccggtcg ttcctgcgtg aagcaaaagc ttattccaaa tccggcggcc cggtcaatct
      181 gaaatacgaa gcggtaaaag gcctgttcga taaatcacag aaattgtttg tgcattgcga
      241 cctgactaaa gagatcctca tggccctgga ttttaaaaaa gaatttgacc tcgacctggt
      301 gatcgagggc ggtaccgaca gctggatggt agccgatatc cttcgtgaac accaggtgcc
      361 ggtaatcctg agcgagcccc ataaccttcc ttccatggca gatgacgcgg tggaccaacc
      421 ctataaaacg gcttatctgc tgcacaaagc gggggtgccc tttaccatag ccattgacgg
      481 attctggcag caacgcaatc tccctttcca ggcggggacc ctggcagctt atggactgtc
      541 taaagaagaa gccctgtccg caatcacgct ccaggcggcc cggatcctgg gcatagaccg
      601 gcagaccggc agccttgaag cgggcaaaga cgccaatatc gtcgtgagcg atggggatat
      661 cctcgatatg aaaagcagta aggtgaccca tgcctttatc cagggcaggc agatcaacct
      721 cgacaacaaa cacaaccagt tgtttgagaa atacaagtac cgctacaatc tgaaataggg
      781 aaaatcagac tttgagggta tccacagacc ggtcaatgac ccattgggga accgggatgg
      841 ccgacagttt tgaatctatc caccccttgc agtcctgcaa cagggtggcc ggatcgtttc
      901 cgcggaaagc atgatcggga aaccagatgt ccacgcgggt cctccattta ccgaattgtt
      961 tgataaaatg ggtcaggagg ctgtccccgg gtatccagaa atccagttcg ggtttgtgat
     1021 acaggatggc caggggcacg acgggcactt tttcttccac cgccacctcg aaagcccctt
     1081 ttttaaaatc cttggtcagt tggagtacgc tggtcgtagc ttccgggcac aggaagatat
     1141 tgaagccctg cctcagtttt tgcctcatcc cttcttttac ggaattccgg ctggtacggt
     1201 cttcgcgctg aacgaagagg atccccgttt tttcagccgc caggccgatc aggggatact
     1261 tttcaatctc tgctttgccg atgggaaaaa aatggtaata atgcagagcg atgaccgggt
     1321 cggaaaaaca acgatgattt gaaatatata tggccggctg gatctgatca aatttgccaa
     1381 aggccctgat ttgatagccc agcacccagc ccatcaccct gaccccggct ttccggaagc
     1441 ggaaggccct ccggatatcc tggccaagaa atacggtggt cagcaaataa gccatcagaa
     1501 agaggaagat gacgagcacc aggatgatca ttctggacca ggccagcaca actctcattt
     1561 caggaggcca taaggaatga aaacaataaa cctttgcccg atcagaccag catgcgaacc
     1621 ggttcttcca gcagaccctt cagggtttgt aaaaactggg ctccgctggc cccgtccacg
     1681 acccggtggt cgcaggagag ggtgactttc atgatatggc cgatttcgat tttcccttcc
     1741 cggacaaccg cttgttcggt gatcccaccc accgccagga tgcaggaatc cgggggattg
     1801 atgatggctg taaattcatc caccccaaac atgcccaggt tggagatcgt gaaggtattg
     1861 ccctgcattt cttcgggctg cagttttctt tctttggccc gttgagcgag gtattgcacc
     1921 tcggcattga tttgggtaag ggtcttgatg tcggcatatc tgatcacggg cacgagcagc
     1981 ccttccgcca cggctacggc caccccgata tggatctggt ggttctgacg gatggcgtcc
     2041 ccggtccagg aggaattgac caccggatgt ttgcgtaggg ccatggctac tgctttcacg
     2101 accaggtcat taaaggagag tttggatggg gctacggcat tcatggctgc ccgggcgctg
     2161 accgccttgc tcatgtcgat ggccatggtc atataaaaat gcggggcgga aaacttgctc
     2221 tcggcaagcc ggcgggcaat ggttttccgc atctggctga ggggcttgtc ttcataagcc
     2281 gctcctcccg ggctgatgtt gaatacaggg gccggcgcaa tcggggccgc tgcggccggt
     2341 ttggcgggag cggtttgcgg gctggttttt tgttgaaggg cagcctctac atctcttttg
     2401 ataatacggc ccaggtcgcc gctgccggtc atcttctgca aatcaatgcc ttcttctttg
     2461 gccatggctt tggcaagggg ggatgctttg atatggctgt ccgcggccac gggcggcgcg
     2521 gccacgggcg gcgccgccaa gggcgccggg gcggcctccg tgaccggttg cggattcgaa
     2581 gaggcgtcgg cggcaggagt ctgcccggcc gcaggagccc ctgccgcttc aagggcggat
     2641 ttataatcct cccccttttt tcctattacg gccaatatgg cattgacggc aatcgttcct
     2701 ttttccacgc cgatataaag aagggtgccg tcataaaaac tttccaggtc catggtggct
     2761 ttatccgtct ccacttccgc cagcacatcg ccaactttta catcatcacc cacttttttg
     2821 tgccatgcca ggatattgcc ttcttccatg gtatcgctca tccggggcat tctgataact
     2881 tctgccatat ttgatctgtt ttgaggcgta aagatagccc ccggcagcca tttttagggc
     2941 aataactgaa aagtccttcc ggggtagagg cccgtaattc ttaattctta attattaatt
     3001 ccttcattat aaatttgtcc ggttgtatga aattttcttc taaactgctg gaagaggcca
     3061 tgatggcttt tcattccctt cccggcgtag gcaagaagtc tgccctgcgg atggcgcttc
     3121 atatgatgaa aaaggacccg gaatatgccc gaaaattttc cgaagcggta tggaatgccc
     3181 gcaataaaat aaggacctgc ctgcgttgct gcagttattc cgacgaagag ctctgcagca
     3241 tttgtgcaga cccgcggcgg gatgaacata ccgtatgcat cgtcgaatcc acccgggatg
     3301 tcatggccat tgaagaaacc aatcaatacc atggagtcta tcatgtcctg ggaggggtat
     3361 tgtcacccat tgacgggata ggccccgccc aattgaatat cgaagccctc acccggcgcc
     3421 tggcctcaga ggagatccgt gagatgatca tggccatcaa cccgaccatt gaaggggaaa
     3481 ccacgattta ttacctgtcc cgccaggtac aaccgttcaa tattccggtc accgtcattg
     3541 cccggggcgt atcttttgga ggtgaactcg agtatgccga tgaaatgacc ctgggcaggt
     3601 ccatcctcgc ccgtacgccc tatcaaatca agtccgtcac ctgaacatgg acctcacggt
     3661 catcatcctg agttacaacg tcaggtatct gctggaatcc tgcctggagt ccgtggaaac
     3721 ggccatgcgc cggatccagg gggaaatcct cgtggtggac aatgcttctt ctgatgaatc
     3781 ggtggagatg gtgcggtcgc ggtttccgga ggtccgcatc ctggccaatg atcaaaatat
     3841 aggattcgca aaagccaata acctggccat caaacaagcg agggggaaat atgtactgat
     3901 cctcaacccg gataccctgg tgcacgaagg cgccttgaga gcctgcctgg attatctgga
     3961 atcagaggcc gatgtcgctg ccgccggggt ccgcatgacc gaccatgccg gccgtttcct
     4021 tcgggaatcc aagcggggtt ttcccggcct gtggccgtcc atctggaaac tgagcggctt
     4081 cagccggctc atgccagggc atgcaaaaat caaccattat tacctcgggc acctggatga
     4141 ggccggcatc catcccgtgg aagtcctgac cggcgccttt atgatggtga aaaaaaccgt
     4201 cctggaagag gtcggcggtt ttgatgaacg gttttttatg tacggggaag acatcgatct
     4261 ttcctttcgc atccgcaaac tgggttaccg gttgatttat cttggcggcc agtccatcgt
     4321 gcacctcaaa ggacgcagtt ccgccctgca ttccgccgct tacgtcctga atttttacag
     4381 ggccatgtcg gtatttgtaa agaagtatta tgtcaacccg gccctgaaga ccatagtcat
     4441 gaccggcatt ggattggctg ctgtcctgtc ctggctcaaa cgaaagatcg tgcggcattt
     4501 cctgcccctg atggatatcc ttttgctctc cctcctgttg ttcctggtcc agtacctctg
     4561 ggcaaggggg tggtttgaag accctgctta tttccacaat tccgtttttt tgttaaacgc
     4621 cctgagttat gtgttccttt ggttcatggc cctggtctta tttgaagcct accggcccta
     4681 ccaggaaaaa ccggcctggc ttgcgctcaa ggccgtcctg gccggcacct tggtcatcct
     4741 ggtcctctat tccttgctgc ccgaaaactg gaggagctca agagcggtta tcctcatgag
     4801 tggtttatcc gcggccctgg tcctcccctg gagcaggaat ttactaaggc ctggcctggc
     4861 cgccctgcgt gccctttata ccggtgacca ggccggggaa aagagcctga acccgttgtt
     4921 taagagattg tgccacgatc attatttttc ttacctcagc cgggaagacc ttagcggact
     4981 gagagaaccg gaaacccggg tggctaaact caaagaccag gtgcaggccc aggatattac
     5041 ccatgtcatt cttgaaagtc ccgccaaatc gaaagaagac ctcctccttt ttacccgcgg
     5101 attgaagggc agggtaaaat tcctggtccc tgaaaaaaac gggtggccgg agaccggggt
     5161 catggcgtcg attcccggat tgaaagaggt ggatatccgc ctgggcaagc ccatgtacag
     5221 aacaatgaaa tggttgttca atttcgtcct gaccgtgctc attctgccct ggggctggct
     5281 gagaagggat atccggaaaa attattttgc tctgctcctg ggcaccaaac aatggatctc
     5341 ttatcatcag ccgccggatc cgaggttgcc gtcccaaagg cccggcctct ggcatccggg
     5401 cttcgacccg gtgacgggcg acgactatcc ctggcaggac ctcaattttg aatatgccgg
     5461 aaaatatacc ccgggccgcg atatcctgat tgtcctggcc cgattgttta attttaacta
     5521 atgaatgaaa tgctcagtcc tgaaaaaatc accggcatgt cgaagcaata cctcccggag
     5581 accatcaggc tccgacggta cctgcatgaa cacccggaat tgtcattcca ggaacaccag
     5641 acggccgcat ttattgaaaa caccctccgc gcttatggcc tggaacctgc gcgaatggcc
     5701 aataccgggg tgatcgtgct gattgaagga caaaaaccgg gcgggaaagt ggtggccctc
     5761 agggcagata ccgatgccct gcccatccag gaaatttcct ccgaacatta ccggtccagg
     5821 aatccggggg tcatgcatgc ctgcgggcac gatgtccata ccgcctccct gatcacggtt
     5881 gccaggatcc ttcatgaaac gaggccggcc tgggggggaa aggtgaaaat cattttccag
     5941 ccgggagaag aaaaatttcc gggcggcgct tcgctcctga ttggcgaagg ggtgctgcaa
     6001 aacccggtgc ccgaaatcat cctggcgcaa catgtttttc ctgaactgga agccggaaaa
     6061 ctgggtttcc gccagggacc cttcatggct tccgcggacg agatctatat caccgtgcac
     6121 ggcaagagcg gacacggggc catcccgcat tcgacccgcg accccatcct gattgcctcc
     6181 catattgtgg tcgccctcca gcaaatcgta agccggaaca acaacccgct gactccttcc
     6241 gtactgacca ttggaaaaat ccagtctacc ggcggggcca ccaacatcat ccccggggaa
     6301 gtcaaaatgg aagggacctt ccggactttc gacgaggcct ggaggaaaga agccaaatcg
     6361 ctgatccgta aaatcacgga gggcacggcc caggctatgg gcggtcaggt ggaaatcgat
     6421 attaaagacg gttatcccgt cttgctcaat gatcccaaac tgacccaccg gatgcaacag
     6481 tgggccattc aactgctggg tcccgaacag gtggtggacc tgcctatgcg caccacggcc
     6541 gaagattttg cttattattc ccagcttatg ccggcttgtt tttaccggtt gggaaccgcc
     6601 catccgggtc gacccggtga aaagcgggta cataccccgg actttgacat cgatgaagag
     6661 gcgctggcca ccggggttgc ctttatggcc tgggcggcgg tcagttggtt aaaaactgcc
     6721 tgatctatac atataacaaa ttataatatt taaaaaatta atattactac tagtatagat
     6781 tattttttta tttaatgtat tttgggcctt gattaatgta atggattatg gcaaaaattt
     6841 tgatcgctga cgatgagcgc agcatccggc gaacgctccg ggagatcctg gaatttgaaa
     6901 aatttaccgt ggaggaagct tccgatggac tggaatgcct cagtaaaata aagcagcagg
     6961 attttgacgt cgtcctgctg gacatcaaaa tgcccaattt ggacggcctg gaaactttgg
     7021 aaaagatcca gcagctcaac cccgatctgc ccgtagtgat gatcagcggc catgccaaca
     7081 tcgatacggc cgtggacgcc gtaaaaaaag gggccttcga ttttatcagc aaaccgccgg
     7141 acctcaaccg tatgctgatc accatccgca atgccatgga caagacctgc ctcattaccg
     7201 agaaaaaggt gctccagcat aaaatcagta aaagcaggat ccaggaaatt gtcggggact
     7261 ccccggccat tgccaaagta aaggaaacca ttgaaagggt cgggcctacg gaggccaggg
     7321 tcctgatcac cggggaaaac gggacgggga aagaattggt ggcccggtgg atccatgaat
     7381 acagcgggcg caaacagggg ccgatcatcg aagtcaactg tgctgccatc ccttccgagc
     7441 tgatagaaag cgaactgttc gggcatgaaa aaggctcctt tacttccgcg atcaaacagc
     7501 ggctgggaaa atttgaactg gcacacggcg gcaccttatt cctggatgaa attggggata
     7561 tgagcctgtc cgcccaggcc aaggtgctca gggccttgca ggaaagccgc atcacgcggg
     7621 tgggaggcga ccgggaaata aaagtggatg tgcgggtggt cgcggccacc aataaggata
     7681 tgcggaggga aatcgaagcc ggccgtttcc gggaagacct ttatcaccgg cttgcggtga
     7741 tcatcatcga ggtgccttcg ctcaaccagc gcagcgagga tatccccgtg ctggtggaac
     7801 attttaacca gctgatctgc caggaatacg gcatacccga aaaagggttt acggacaaag
     7861 ccatcaaagc cctgcaaaag gtgcaatgga gcggcaatat ccgggagttg aggaatgtcg
     7921 tggaaaggct catcatcctg agcaccggcc ggattacgga caaggaagtt tataattatg
     7981 tcgttccgac cgccctgcat ggcagccgtt accgtgaaat atttgaccgg ttccaggatg
     8041 tccgggacct ccgggttttt atcgaaaggg aatacgaaca gtataaagcg gaatccgttt
     8101 aagccggttt aaaaaaaacc agataatcat ttgatgccat gacccggttt caaagggaaa
     8161 tgaaaaaatg gaccggcctc cggggggaaa attcctggac cctgttcaaa gtgatggccg
     8221 agatggtgga tggatttgaa gtcctcaacc gggtgggacc ctgcatctcc atttttggtt
     8281 cggcgaggac ccggcccggc aatttttact ttgagaaagc atcagaaatc gcccaccgcc
     8341 tcaccgacga aggatacggc atcatcaccg gcggagggcc gggaatcatg gaagccggca
     8401 ataagggggc tttcgatgcg aaagggacca gcgtgggcct gagcatcgac ctgccttttg
     8461 aacagaccta taatgaattt gtggacccgg acaagatcct caaacaccgc tattttttta
     8521 tccgcaaagt catgtttgta aaatatgccc agggctttgt agtcatgccc ggcggattcg
     8581 ggaccctcga tgaatttttt gaagtgctca ccctggtcca gaccaaaaag ataaaccggg
     8641 tacccatcat tttattcgga gtaaattact gggaagggct gaggaaatgg cttgttgaaa
     8701 ccgttgacca ggaagcgcac aacatcaacc cggaggatat ggacctgttt ttgattaccg
     8761 acgatgtgga atcggtggtg cagcacatca acgcgtttta cgatgcccgg gacgatcatt
     8821 tcaggcctaa ttacgagttg taagctccgc ctttttttca cccctctccc ttcgcctttc
     8881 gcctttcacc cttcgccttc aatccctgag cgtccaggta aaccccatcc tgatattcgt
     8941 cctgaataaa ggctggtcgt acgtctcata aaatgtcttt cccgcagtat aaaacaaata
     9001 ctcaagccct tcgatcttaa aaaagcctct gaaattcctg agctggaaat tgaaaaaata
     9061 ttccaggtag gggtggagat ccactggagc ccggtccgca ttgtagaagg aggcaagcag
     9121 gggaaaatag gcggttgacc ggaaaccggg aatgatccgg gtctccactc cggtgagcac
     9181 ccccatagcg ttcttaaacc aggagccctt ataatacaat tggtgcctgg tatgccaggt
     9241 tgggatcctg aggatctcct tggccgatga aatctggtat gtgaaggcgt gcatgctgtg
     9301 cattttccca aaatcaaagg ctttgtcaaa atacagcgta gtgacgctca gggccgagcc
     9361 ttcctggacc ggcctgcgaa ggctgtccag gtagatcgta ttggaaagga tggactggtc
     9421 gatccccgcc ctgaaggaaa gggcggcgac ccttatcgat cctccgatct ggttggtcag
     9481 cggtttttca aaatcgttgc tgaacacgag ggtattccgg ttgtacattt cggcgtaagc
     9541 aaggggtacc ggtgaccgct gaaggatgag gtggccttcc aggctgccga gtttccggct
     9601 tgcagcctgg acctgtcctt tcaaataata ttctcccagg ttgtcgagca gcccccactg
     9661 gccctccgca ttcaattgca gggtcccgaa cagggattgc tgatactggc ccaaagcgga
     9721 aaggaggttt ctcctgaatt ccgccggtcc acggtcgatg gtgtaatatt catgtttcag
     9781 tcctcccatg aaataatttt ttcctgccag caggccgtcc aggcgaaatt cattcaccag
     9841 cccgcccgca cggaggtcca gttgctgcgt atcccgcaca taaaaggggc cgtaataaaa
     9901 ggagtcgttc ggggaagtga aatcttcata gaaaatggtt tctttacgaa gagctatgcg
     9961 gtgcatggct cccaggatat tgcccttcca tttgaggttg aatccgtttt gaacactgaa
    10021 ttcattattt cgctgccggg aacgtccgtc aagcaatacg tcgagggtct cggtggcgcg
    10081 ccggtattgg gagatggtga gcaggctgtc ggcaaagggc accccgccgt tgtcctggtt
    10141 gcggatatcg ttggtcaggt aacttacgat ggcatcgtag ttgcccttgg gcgaatgata
    10201 ccacaggctg aaggccccgg tccggtcctt ggtgatctgt tcggtgaatt cgcccttgta
    10261 ctggatggaa ctgtaattca ggtccaggtg caaaccgttt tgaaaggtct tgctgaagcg
    10321 cgattccagt tgcaggtctt cctggctgaa acttttctgg gcaaaacgga aaatcgcaaa
    10381 agggcgcctg gaatccagga aaagcaggtc ctcgtaatct ttccggtaga ggtcgaaact
    10441 gcgcaggcct aaatccaggc cctgtctttt atccaggaca tagccggccg ggcggtgggg
    10501 cgacccgttg ttgccaagat ggaaataagg aatgccctga agcctgatgg catcgtaatg
    10561 atggaaaagg ggatccagca gggtatcgtc aaaaaaaacg gccgttttca accggtaggg
    10621 tgtatagatc cggaccgtat ccgggggttc atccgtcaat tcttcctgct cgatcgaatc
    10681 ggggacttgt tcctcaaccg acctggggaa attggaaggc aggttgcgct gcgcctccag
    10741 tgcggaaggc aggcccatcc agcccagcag gcaaaggaaa agcagtcttt tgaccaaaga
    10801 catccattta tttttcagaa ggggcctcct ctgcgaagaa aaggggactg atgcccatgc
    10861 tggagaagcc gccgtcgtga aatagattct gcatggtgac catccgggtg agatcggaaa
    10921 acagggtgac gcaataattg gcgcaatctt cggccgtggc attgcccagg ggggacattt
    10981 tctgtgcata atcaaaaaat tccctgaacc ctttgacccc gctgcccgcc gtggtccagg
    11041 taggggactg ggaaatggta ttcaccctga ttttcttaag cgtgcccagg tgataaccaa
    11101 aactgcggcc gatggattcg agcagggctt tggcatcggc catttcattg taatcgggga
    11161 ataccctctg ggccgcgata taagtcaagc caagcaccga accccattcg gcaatgccgt
    11221 ccgtcttcag ggcgacctgc atcattttgt ggaaggaaag ggcagaaata tccatggtct
    11281 tgagcatcca ttcatggttc aggtcggtat aatgttttcc tttgcgtaca ttgagggaca
    11341 tgccgatgga atgcaggaca aaatccacag gccctcccag gatttctttg gagcgggtaa
    11401 agaccgtggt gaggtcctcc atggaagtgg catcggcagg gatgatttcc gattgcagct
    11461 ttgaagccag ttcattgatc ttgccgaagc gaagggctac gggggcattg gacaaggtga
    11521 tccgggctcc ctcctcgacg gcccgttcag cgactttcca tgcgatggac tgttcgtcca
    11581 gcgctccgaa tacgattcct gttttgtttt tcagaagttg attgctcata cctgatgctg
    11641 ttttatacga tttcaattaa ttctttagcg ttcttgatgg cggctgcggt aggcgggttg
    11701 gtgctcagca tggcggcgat cgtttgcacc cgctcagcct tattgagttt tttcacccgg
    11761 gcggtggtgc gctccgccga ttcatccttg tacacaaaat aatgaaggtc cccctttgct
    11821 gcgatctgtg gcgaatgggt gatgcagatc acctggtgat ggaccgaaag atctttcagg
    11881 atacggccca ttttcagggc cacatctccg ccgatcccga tatcgatttc atcaaaaatc
    11941 aaagtgggca ggggaatggc ctgggcgacc agggatttgg tcaccaggtt aaaacgggcc
    12001 agttcgcccc ctgaagcgat ttgttttatg ggcaggaagg ccccgccttt gttggtggcc
    12061 aataaaaaat cgatctggtc cagacccgtc tggttcaggg aatccaatgg gctgagttca
    12121 atccggacct gggtattttc catggccagg ggccgcagca aaacataaat tttttcaata
    12181 aatccgggaa tgactttttc ccggcgggca tgcaattccc gggctttacc cagcagggag
    12241 gtttcctgct ttttaatttc cttttcgagg gcggccagtt gttctcccag gtccgaggcc
    12301 atgtgcagcc tggaggagaa ttgctgatga aggactataa gttcttcaac ggtttttacc
    12361 ttgtgttttt gacagagcct gaaaagggag gacagccttt cttccacctc ggccagccgt
    12421 ccggggtcca ggtcaatttg ttcgctgatg tttttacatt cggcgctgat gtcttccagt
    12481 tcagcaagga tggaatggat ccggtccgat atggattgca atccccggtg cacggatcca
    12541 agcgggtgca ggctccggtc aaggtcgctg agcatgccgg tcagggatct ttcgtcttcc
    12601 gtgagcgcaa ggtggatatt ctcagaggcg gtctttattt ccccggcatg ggccagagct
    12661 ttttgttcgg tctccagggt atcccattca ccggccacaa gattcaattc atccagctcc
    12721 ttccactgga atttgatgaa ttcggcttcc tgttcttccc tcgtgagccg gtcttttaag
    12781 gcccgtaggg tttccaggtt ggtggtatac cgtttatact cctgttgata ttcttccagc
    12841 aaggctttgt ttccggccaa agcgtcgatc atcctgagct gggtgctcac ctggtagatg
    12901 tccagagtgt cgaattgctg gtggacatcg atgagggcgg cactgagggt ttgcagaatt
    12961 tccagggtga ccggggtatc gttgatgaag gcccgggatt tgccctgggg ggagatttcc
    13021 ctgcggatta cgatctcatg gtcgaaatcg aggtcctggg cttcaaaaaa gggttttaac
    13081 tcctctcctt taatcataaa ttccgcctcc accacacatt tggccgtctg attaccgatc
    13141 gcccggatat ccgcccgttg accgagtacc aggttgaggg ccccgatcag gatggattta
    13201 cccgccccgg tttcgccggt gatgatgatg agatttggtg aaaactccag ctcaacatcc
    13261 tcaatgatgg caaaatattg aattttgaga cggcggatca aggtattcct ggatttgggg
    13321 caaaggtaag gaataggtgg gaggtcgaag accggaggtg ggtggttcgg tgggtggaag
    13381 ttttttagat ttaactttca gtcaaatgtc taactttgta gggaatgaag accagcgcaa
    13441 gggaattggc tgaattttta catgccacca tcgatgggga tccggatatt gaggtcactc
    13501 atcccagtaa aatcgaagaa gcgggtccgg gcagcatcag ttttataggt aaccccaaat
    13561 atgaatcctt cgcgtatacg acccgggcat ccatcctggt cgtccccaat gaatttaaag
    13621 cccaacggcc cctgcaggca accctgctcc gggtggacaa tgtctaccaa agcctgggca
    13681 acctgctgag ccggtttgaa aacaatggcc atccggtagg gatttctgac caggccagta
    13741 tcgatgagac cgtcagcctg ggagccggtg ttgctgtcgg cccgctatcg gtgatcgaaa
    13801 aaaatgccgt gatcggtgcg ggctccctga tctatgccca ggtttatatc ggggaacagg
    13861 tgaccatcgg ccgggattgc actatttatc cgggcgtaaa aatctatgca ggctgcaagg
    13921 tcggggaccg ttgcgtgatc cacagcaata cggtgatcgg cagcgacggt tttggttttg
    13981 taccaaaccc ggaaggccgg atggaaaaaa taccccaact tggcaatgta gaactggggg
    14041 atgaagtcga aatcggttct ggatgcacga tcgacagggc taccatgggc agtacccgca
    14101 tcggtaaggg ttgcaaactg gacaacctga tccagatcgc gcacaacgtg gagatcggcg
    14161 agcataccgt gatcgcggca caggccggca ttgccgggag caccaagatc ggggcccgtt
    14221 gcatgatcgg gggccaggta ggcattgcgg ggcacctgcg cattccggac ggtacccaga
    14281 tccaggcgca gagcgggatc atgtcggcgc cttccgaacc caatcaaaaa ttattcggtt
    14341 acccggccat ttcctatcac gattatctca gggcttatgc ccttttcaga aagcttcctg
    14401 atcttgaaca gcggttgcgt gaccttgaaa aaaaatccgg agaataattt attgatcgtt
    14461 ttgatcttcc tttccgtata aacttcttta gaaacctaat agtttttaac ttaattttgc
    14521 attccctggc tggtaataag gcataggtca tcgcctggcg accgtttata acctatccga
    14581 caaccagaca gagaaagaca ttaacatgaa gcagtgtacg atagccaggg atgcggtttt
    14641 tggaggcatt ggattacata ccggaaaaag cgccaccatg acgatccgtc cggccccggt
    14701 gaatcatggg atccggtttc aacgcattga cctcaaggat tcacccatcc tgcctgccga
    14761 tgtcaaccgc gtaatctcca ctaaccgggg cacgaccatc aaatccggcg atgcccaggt
    14821 gagtacggtc gaacatatcc tttccgccct cgccggcatg ggaatagaca atgtgctgat
    14881 cgagattgac gggcctgaag tccctatcat ggatggcagt tcaaagccct ttaccgacgc
    14941 ttttatccag gcagggatta ccgttcagga actggaaaag gattacctag aactgaccga
    15001 aacggtggct ttcaaggatg aactgagcgg cacggagctg atcgccgttc cttcggacaa
    15061 gtttgaagtg accgctatga tcgatttccg gtcccctttc ctcggacaac aatatgcaag
    15121 cctgaacgac ctctcggaat atgaaaaaga aatcgccccc tgcaggactt ttgtatttct
    15181 ccgggaaata gagaagctgg ccaatgaaaa cctcatcaag gggggagacc tgaacaacgc
    15241 catcgtgatc gtggaccgca tgatgaccca gccggaactg gacgacctgg ctcagaaact
    15301 caataaaccg tccatccagg tggaaaagga gggtatcctg aacacggtcg aactgcagtt
    15361 ttccaatgaa gccgcgcggc ataaggtgct ggacatcatc ggggaccttg ccctcgtcgg
    15421 caagcctatc aaagcgaaaa tcctggccaa caaacccggt cataccgcga atgtggaatt
    15481 tgccaaactg cttaaaaaaa agtacaatga aaaccggaaa ctcaaaggca aacccaatta
    15541 caatccggat gaaacgcctt tgatggatac cgaaaggatc cagaaactgc tgccgcaccg
    15601 gcatcctttt ctgctgatcg ataaaatcat cgaactgagt gaccagcacg tggtaggggt
    15661 gaaaaacatc acctttacgg aggcctgttt cgccggccac ttccccggta acccggtttt
    15721 tccgggtgtg ctccagatcg aagcgctcgc ccagaccggg ggtatcctgg ccatttcgca
    15781 gcaacccgat ccgggcaact gggatactta ttttatcaag atcgaaaaca ctaaatttaa
    15841 aaataaggtg atgccgggag atacgctgat cctgaagatg gagctgatgt ctccgatccg
    15901 aaggggtatc gtacagatgc ttggtactgc ctatgtggga aataaaattg tttctgaggg
    15961 cgaattgacc gcccagatcg tcagaagaga atcaaaccca acataaccat ggacatcgtg
    16021 acccaatatc agctgaatgt agtccatccg caggcgcgga tcggaaaaaa tgtgcatatc
    16081 tcgccttttg ttacgatcga aggggatgtg gagatcgggg acaattgctg gatcggtccc
    16141 aacgcttgca ttctggacgg cacccggctc ggtgaaaact gcagggtttt cccgggggcc
    16201 gtggtcgggg cagttcccca ggacctcaaa tacagcggcg aacaatccat tctcgaaata
    16261 ggcaacaatg taaccatccg ggaatattgt acgctcaacc ggggtaccaa agccaactgg
    16321 acgaccacca tcaaggacaa ttgcctgttg atggcttatg tacatgtggc ccacgattgt
    16381 gtgctggaag aaaatgtaat cctggccaat aatgtcaacc tggccggcca tatccatgtc
    16441 ggcaaatatg cagtcctggg cggcctgtcg gcggtgcacc agtttgtaaa aatcggggac
    16501 cattccatga ttggaggggg atccctggtc cgtaaagatg ttccgccttt tgtaaaagcg
    16561 gcccgcgagc ccatttccta tgcggggatc aatgcggtag ggctcaagcg gagaggcttt
    16621 tcaaccgatt ccatcaaaat catccaggac agttaccgca ttctttttgt gaaagggtac
    16681 aatacctccc aggccctgga aatcatcgaa aagcagatac ccgattcacc cgaaagagcg
    16741 aggattttgg aatttgtcaa aacctcggaa agggggatca tgaaagggtt ccggcagatc
    16801 aacggcaatg gaagtaagga ttgatcgcct gtccaaaaga taccagcacg gatgggtttt
    16861 ccgcgatttg acccatgact tcaggaacca ccagtgctac ggcatcgcgg gccgcaacgg
    16921 gagcggcaaa tccacgtttt taaaaatcat ttcaggcctt ttgtcagcca cccggggaga
    16981 ggtcaattat cacctggggg gccaaaccgt ttcgagggaa aacatctatc aggaggtgaa
    17041 tgtggccgcc ccatataccg atttgatcga agaattcact ctgagggaga tggtccgctt
    17101 ccattgccgc ttcaagccct tgagcgttga cagggcggag gactggattg aaatgaccgg
    17161 actctccagg gcggcggacc ggcccttggc tttttattcc tccggcatga aacaaaaagt
    17221 caaactggcg ctggccatgt attcccgggg atcgctcctg ctgctggatg aacctacctc
    17281 gaacctggat gacaatgccc ggtcctggtt ctttgaccat ctggaaaaac aaaaagggaa
    17341 caaaacggtc tttatcgcct ccaatgaagc ggaagacttc aggatgtgtg aggaagtgat
    17401 tcggctggac gcaacgactc cttaaatcgg agcatctcat ttttaagatg aatcagcttt
    17461 tgcagcaccg cctgtttttg tttgagcagg tccttgtttt ggcggatttc attcttggcc
    17521 ccttcgatgg tgaatccccg gtttttgacc aggtcaaaaa tgagttgaag ctggtcgatg
    17581 ttttctttgg taaacctacg gtcccccttg ctgtttttat gcggcttgag gatgctgaac
    17641 tcggattccc aatagcggat cagagatttt gacaccccaa acatatcggc gatttcacca
    17701 atgctgtagt agagcttttc atctttgagc gctggaagca tattcaacaa aacggattaa
    17761 taatgatcta cggtacaaaa ctacgatcta aatgtaagaa aaaagccttt ctcttttttt
    17821 ctggttttga ttcccggaat ggtatatttt aaagttggcg gataattaat tgtttatttc
    17881 ctccctgaac tccttgcgaa aaactccgcg acctcttttg ggccggctca accggccagg
    17941 taacggttca gggcctcctg gacctccttg acaggaatgc ctggaaattt cttttttatt
    18001 tcccccagga aaaaaccaag cagctttttc ttcccctttt taaactcgtc cgcttcaacc
    18061 gggaaagagg aaaaaagatc ctccacaacc ccggggatat caaattgccg ggcggcgtcc
    18121 agtttttctt tcaccaggtc ttccagggaa gcttgggagg aggtcgttat ttgaatccag
    18181 acctcctggg cctgctgaag gttgagcacg ccaagccgaa cctgggaaag gaggaagccc
    18241 agcgacttca ctccgaaggg cagctcgccg atctttttat tttgttccct gagccagggc
    18301 agcatggtct ggatgagcca gttggccgcg gatttgttaa gcgcaggctc ttcctgtacc
    18361 agttccacat aatatcgcgc cacctcggga tcctccgcca atacctgggc atccgccagg
    18421 gagaggtttt tttcccgggt gaaaagccgg agatattccg aaggcagggt gggaatactg
    18481 tttctccagg tatccagctg ggcctggctg atctttattg gaggcaggtc gggatccgga
    18541 aaataacggt aatcgtgggc gctttccttt tggcgaatgg ggaccgtgac gccttcttcg
    18601 ggtacaaatt gcatggtctg cctcaccacg gcctgtcccg cttcccacag ggcgatttgc
    18661 ctggctgatt caaactcaat cgccttccgg gcaaaacggc gggagttgat gttcttgacc
    18721 tcgcacctct ccctgtattc ttccgaaccg gacgggcgaa cggatacgtt gacgtcacaa
    18781 cgcatggacc cttcttccat attggcatcc gagatatcca ggtaacggac caggcgctgc
    18841 aggtcggtca ggaaaacggt cacttcttcc gccgtcctga aatcgggttc cgtgaccatt
    18901 tcgaggaggg gtacgccggc gcggttgaga tcgatcctgg accacaccgg atccgtgtcg
    18961 tgcacggatt tgccggcatc ttcttccata tggatatgat ggatccggat ggttttggac
    19021 ccctggctgg tctccaggtc catgcggcct cccaggcata ccgggctggc ctgctgcgag
    19081 atctggtagc ctttcgggag gtcagggtaa aaatattgtt tgcgttcaaa ccaggaatgg
    19141 gtgttgatgg tgcaatccag ggccaggcca agcctcacgg ccgattccac ctgtttgccg
    19201 tttggcagcg ggagggtacc cgggtgagcc agggagacat aacccgtctg ggtgttggct
    19261 tcagtggaaa agcgggcagg gtcatcgcaa aacgctttgg tgagcgtatt gagctggatg
    19321 tggatctcga gtccgataat ggtttcccag gaggaggtgc tattcgtcat gaaaaagggg
    19381 tcagcgatta ataaaatcca aaactgatca gggccgtaaa gaaaataaaa aaggaaaata
    19441 aaaccaggta ggccaggccc agggtaaaga atttgaacac cgaccggatg atcccctggc
    19501 ggtaataacg gatccaggct atcagaccat acaggatgac ggtcaccatg accggcagcc
    19561 agaagaattc aataaagtcc ctgaacaaaa ggaccagcaa ggacacgatc aagaaagaaa
    19621 acgcgtgcca gtgatacagg aaaaaaaggt gttcgatcag gtaccttttt ccccgcaaaa
    19681 actggacttt cataaaaagg gcgaggatgg ggatcatcac aatcatcatc cagctgaaat
    19741 tgccaatgat aaaccggaac atgcctttcg ggtcaagcat aaattttttg gcctgcccca
    19801 ccatcagcgt cgtccagaag gagctgatat catattttgc cgtaatggaa tctatgggca
    19861 gcaggaccac atcttcggga tccagatttt gaaagcccag gacactgatc ctgcgggtgc
    19921 caatgctgtc cggccctggg gcgtttgaag tatcgatatt gaaccagctc atcaggctgt
    19981 cgatagccat cctggatacc cgggtctgcc ccgactcggc atgccgcgcc agttccgtcc
    20041 tgatcttgtg ccgggtgagg atgtcgttgg tctggttgtt gacttcatca aagatccggt
    20101 cgtaaggaat caaaccgctc aataccccga gatggatcac caggaggaaa aagaaaaacc
    20161 ggaggggatg gtaatacctt ttgcgcaggc cattaaaata gtcgatggtc aactggcccg
    20221 ggatccagat ctttttcagg gtcctgaaaa aactggaatc caatgaaagt acatggtcaa
    20281 aaaaatcgct caggaattcc ctgaccgttg gtttgtccgc gccaaaagtc tgtccgcacg
    20341 acgaacagta tttcgcgtcc gtggtttctc tgtaattgca gttggggcaa accatgtcgg
    20401 gttgccaaag ataagaagct tttcagggcc ggctgcttag tcgagggatt ggttttgctg
    20461 ctccgacatc ttcaccagct cctggtattc ggagggggtc aggttgtcga ggcagaaatt
    20521 gatcgggttg atgggggtgc ctttgtacct cacttcataa tggcaatggg gagccgtgga
    20581 ggtgcccgtg cttccgatca cgccgatctg gtttcctttc ttcaattcct ggcctacctt
    20641 gacgtctatc cggtccatat gggcgtaaag ggtgatatac ccgtaaccat ggtccacttt
    20701 gacgcatttg ccatacccgg aatagttggt ctcaatatcg atcactctcc cgttgcccgt
    20761 aacctggatg ggagtcccct tgggagcgga aaaatcaatg ccggtgtgca tttttacgat
    20821 tttatgaacc gggtgcagcc gctttccaaa gccggaaagg aagcggaggc ttttattgag
    20881 cttgtcgatc cgtaccggtt taatggaggg gatggaagcc agcatttctt ctttgtgggc
    20941 ggccacatcc tggagcttat cgagggattt ggattgcaag accagctgcc tttcaagcct
    21001 ttccattttt tcagcggttt taccgaccag ttctcccgaa ctgggcaggc ttctgaaatt
    21061 ttcgtacagg tcgtggcctc cttttccccc atcccagatc gcttcatcga tggggtccat
    21121 acccaccagt tgccggtgaa cgtctgcgtc ccggcgctgg atattggcca gggccttgga
    21181 catcaggtca agctggtccg acatgatatt gtagtggtcc ttgacggatt ccaactcttt
    21241 catcaggagt ttttcattgg gagaaggaag gtatttatga aaaacaaaaa acacgatgag
    21301 tccgcttacc agggcggtgg acaaaaagga aaagaccctg aacaatttta tcttcagggg
    21361 ttctacggct ttctcaaact gcagggtatg gtggttataa acgtactttg taggctttcc
    21421 tgccatgatc gacaaatcat taaaaattaa acctattaat tcaatggata tgaacggatt
    21481 cccggatcgg gaaatagttc atataaattc cggcgggatt ataactgggt aaatttatag
    21541 aagtaaaacc attttaccaa gcacatagta cgcggagcgg agattttaat atatatgttt
    21601 agcacgaatg tgtaagaagt tagtaagttg gtaagttagc aagttagcaa gtgagaaacc
    21661 ggtcagaatc gcaaagtctg cccctgcttc tttatttttg cggcttattt atactagaaa
    21721 aaacatgact tcttcggaaa taaggcggta ttttctcgac ttcttcagat ccaaacagca
    21781 ttttattgaa gaatccgccc ccattgtgct caagaacgat cccagccttt tgtttaccaa
    21841 tgcgggaatg aaccagttta aggatttatt cctgggcaat aaaccggtgg tccatccccg
    21901 gatcgccgat acccaaaaat gcctcagggt gtccggcaaa cacaacgacc tcgaggaggt
    21961 ggggctggat ggcacccacc ataccatgtt tgagatgctg ggcaactggt cttttggcga
    22021 ctatttcaag gaagaagcca ttgattgggc ctgggaattg ctcacccaag tctaccggat
    22081 ggatccggaa cgactgtacg ccagcatctt caccggcgat gaaaacgaaa agctggtcag
    22141 ggatacggaa gccggggctt tctggaaaaa acacctgccc gaagaccgga tcatcctggg
    22201 gtccaaaaag gacaatttct gggaaatggg agataccggg ccctgcggtc cctgttcgga
    22261 gatacatgtc gaccttcgga ccgaagagga gaggaacgcc atcccgggcc gtgacctggt
    22321 caataccggg catccccggg tcgtcgaaat ctggaacctg gttttcattc aattcaaccg
    22381 gaaatcggat ggccaactgg aggaactgcc agccaaacat gtagataccg gaatgggttt
    22441 tgaacggctc tgcatggtgc tccaaaacaa aacagcgacc tacgatacgg atattttccg
    22501 cggcctgatc gcccatgtgg aaaaggcaac cggaatcgcc taccggggtt cttacgatcc
    22561 aaccgccaaa tccgatatcg ccatgcgggt actggcagac catatccgcg ccgtggcttt
    22621 taccattgcg gacggcgaat tgcccggcaa taccggcgcg ggttatgtga tccggaggat
    22681 cctgcgcagg gcggtgcgtt attattattc atttttaaac ctacgccaac cgttttttta
    22741 cacgctggtg ccgatcctgg ccggatattt taaggaggtg tttccaggcc tggacaaaca
    22801 ggaaggtttt gtgagcaagg tcatcgaaga agaggaaaag gcattcctgc gcaccctcga
    22861 aggcggactc cggcgcctgg aatccctttc caccggttca gagattttgg acggctccgc
    22921 cgcttttgaa ttgtacgata cctttggttt tccgatagac cttacccggc tgatctccgc
    22981 cgaaaggaat atgcgggtgg atgaaagcgg cttccaggag gaactggaaa aacaaagagc
    23041 aaggtcccgg gctgcggcgg tcaccgagac cggcgactgg gtggttctgc gggatcagaa
    23101 agaaacccgg tttatcggat atgatcagct ggaggccgaa ggcgtaaaga tcatcaaata
    23161 ccggacggtg aagtccaaag accgggccga acaccagctg gtcctggaca aaaccccgtt
    23221 ttacccggag ggcggaggcc agatcggcga tacggggatc atccaaacgg ccaatgaaat
    23281 catccgggta ctgaatacca agaaagaaat tgacctgccc ctgcacatcg tggatcagct
    23341 gccttccgat ccccgggcgc tgttttttgc ggcggtggac gggccccgga ggaaacgaat
    23401 tcaaaacaac cacagcgcga cccacctcct gcatgccgcg ctgagaaaag ttctgggtac
    23461 ccatgtgcac cagaaagggt ccctggtggc tgaagattat ttgcgttttg acttttccca
    23521 ttttcagaag gtcagtacgg aagaactgac ccggatcgaa cacctggtca atcagaagat
    23581 ccgtgaaaac atcgccgtac tcgaacaaag ggatctgccg atccggcaag cccaggaagc
    23641 cggcgccatg atgctttttg gcgaaaaata cggggaaagg gtacggatga tcacctttga
    23701 cccggaatat tccagggaat tatgcggggg ctgccatgtg tcggcaaccg gaaacatcgg
    23761 cttgttcagg ataaccgggg aatcggcggt ggctgcgggg gtacgcagga tcgaagccat
    23821 caccggcgat gcggtggaaa gatatatcga agaacaaaaa caactgatgg accgtatccg
    23881 ggaagcgctt aaaaatccca aagacgtact ccacgcgctc agccacctgg tggatgaaaa
    23941 caagaacctc cggagccagc ttgccgtata ccaggaaaag gaaatcgcaa atctgaagca
    24001 gaaactcaaa gcggaagctt tgctccggga cggggtgcat tatgtgaccg gcctcctgga
    24061 aaacctggat gaaaaagacg ccaaaaccct ttcttttcaa cttgaaaaag aattgtcgcc
    24121 cgcctttatc ttgtttggtt ttgtatccgg aggcaaaccc atgctgatgc tgcacctctc
    24181 cagggaactg gttgaaaaca aaggcatgaa cgcggtccac ctgatcaaaa gactggccgt
    24241 gcacatcgaa ggcggtggcg ggggccaggc cttttttgca accgcgggcg gcaaaaatcc
    24301 ggacggcctg acctccgccc tggtggaggc tttgcaatcc gtataaatac gctactcatg
    24361 gataaacgtt ttttggtctg ttgtgcgaca ctccttttaa tgggcctttc ctttgaaatg
    24421 gccgcccagg caagcctttc caggataagg gttgaaagca accggtttgt caatgaacaa
    24481 aatgagaccc tggttttccg gggttataat accagcgatc cggacaaact ggtttatgac
    24541 ggacagtgga acaaaagcta ttttgaagaa atcagggcct ggggtggcaa tattgtccgt
    24601 tttccggtcc atcccgccgc gtggaggagg caaggcaaga acaattacct gcgcctcctg
    24661 gaccagggca tccgttgggc cggagaggcg gggctctacg tgatcatcga ctggcacagc
    24721 atcggcaatc tcaaatccgg ccttttccaa aacgattctt atgaaaccag cctgaaagaa
    24781 acctttgagt tctggagagc catggcaacc cggtataaag aaaatccggt ggtcgctttt
    24841 tttgaactgt acaacgaacc tacggtattc aatggccaac tcggggtctg cagctggagt
    24901 gagtggaaag ccatcaatga agagatgatc accatcatcc gggcccatgg ctgcaaggca
    24961 atacccctgg tcgccggttt caactgggcc tacgatctca ccgtgatcaa agacgaacca
    25021 ataaacgctc ccggcattgc ctatgtatcc catccttatc cccagaaaag ggaaaaaccc
    25081 tgggaagcga aatggacagc cgattgggga tttgcgacgg aaaaatatcc gctgattctg
    25141 acggaaatcg gtttcagcgg cccggaggaa gccggtgccc atatcccggt gatcagtgat
    25201 gaatcctatg gcgaggctat tgtaaaatat tgccaggaga gggggatctc ttttgtggcc
    25261 tgggtattcg atgccaactg gtctccccgg atgttcagcg attggaaatt caccccgacc
    25321 cggcagggga aatttttcaa agaggcgctg acgaggaagt gattagttag atagttagca
    25381 agttagaaag ttggcaagtt tctcgtttct cgcatctcgc atctcgcttc tcgcttctcg
    25441 catctcgctc actgttaaat atagattaac tggcagttca cttataccct ggatcccgtg
    25501 agtcatcgtt tcccgggtaa atcaaatcat gaaaaccaaa atgcggcgga cctggcttct
    25561 ggccgggctt gtcctgggtt cttgccaggt ggaactggat aattttttcg gtaagcaaaa
    25621 cccagggact tacccgaacg tcgacgaagc gctccggcct tattttcaac gattcgaggc
    25681 agaagcggca gccaggggac tgactgtgga cctggccgat gcctatatta ccggttacat
    25741 ttcagatatt cccgagaacc aggttattgg ccagtgttcg tattccagca atgatccgca
    25801 ccgggtaacc attgataaac ctttctggaa ccaggcatcg gacctggagc gggagtttgt
    25861 ggtttttcat gagctgggtc attgttacct ggcccgggtg cacgatgagt ccatggatgc
    25921 ccgggggatt tgcctgagta ttatgcggag cgggacgggc gcctgcaggg atgtttataa
    25981 cgcagcaagt cggagcggtt tgatccagga attatttacg gaaaaggatt gaagcttcag
    26041 tcgtagtaag ggatatcgga caagggatag atcccatgtt ctttcggtgc ggacaaaggt
    26101 cgtgtcgcgc ccagcaggga attgagccgg gattccgtga aggaggggtc tccgcgcacc
    26161 aggctctcca cccttacgta tcccgaggca tggatgatct tgccctcccc ggcataaatg
    26221 gccacatggg tcaccctttc aggatcggtc gccgtcgctt ttcttccaaa aaataaaaaa
    26281 tcacccggtc gcagattttt taaagtggta tcggtttcca ccggggcgcc gaccttaacc
    26341 tgctgcgacg catccctggg cagttgccaa ccgtgttgga acaagacggt ttttgtaaag
    26401 ccgctgcagt ccattccatt tcccgaagtg cctccccaga gatagggccg gcccaggtaa
    26461 cccatggccg tgcgcacgag gtcctctccg gcaggcaggg tgttggcctg aaatttccat
    26521 tcctccagcc aggtggtatg cgaagaaggg atgtagccat ttcttccatc cgggaaggag
    26581 gcctgaataa agtccccccg ggtatcttcc agtttcagga tatttccgga agtaaggggt
    26641 gagagccttt ccgacgagag ggatggggct gaaaacaggt aggtctggtc cgcgatgatg
    26701 atgatctttt gcctggcgat ccaggcttta taggcggcgc tgtccatcag atggacggca
    26761 tcctcgtcca cccagccgag atagtcatcg ggggtctggg catacaacca ggagttggaa
    26821 tacttgtaaa gacgcaatac cgtgccgccc aaggcctgcg tgaccagctc actgctgtgg
    26881 ccgggccggc tgcggatatt ggccaggggt atggtcacca ccccgaaatg ataaccttcc
    26941 agggatggcg aagggagtac catgatgctg tcaacaaaag cgatcccctc caatctccgg
    27001 accaattctt ctttggcccg gccgttatcc acttcccctt ttaagatcag ggttttgccc
    27061 tgaaagacgg ggtcgatatc aaaaatagcg gtccttttgt ccggcgcata tttgtaccgg
    27121 agagaatcca ggatttccgt gacgcgtgca tcgggcaggg gacctgagga acaataggct
    27181 aaaaagaaag gaacggccag aaggagatat ttcatgatac aaaagtagaa aataaaaggc
    27241 cgaacctctc gtctgagatc cggccctgtc cacctactct tagggaagaa tagttcttat
    27301 gtatttttat tcagggttca aatggattcg atggagccgc ttccggagat gccggttttg
    27361 attttcggct ggcctttgta acgaacgctg ccgctgccgg agatgccggc ttcgagcata
    27421 tcgctggccg aaacttcaca ggaaccggaa ccggagatcc caatcttgac ctggctggcg
    27481 gggaaatcga gcgccctgac atttcctgaa ccggaagtgc ctattttcag atcgcgggtt
    27541 ttacccttaa ggtgaatatt gccggaaccg ctgaggccgc aatccacatg gtcggcttcc
    27601 accatgacct tgacggtgcc ggagccgctg acccccacat taaaatcatc cgcggtaaac
    27661 atattggtgg tcacgatcga accggaaccg ccgaggccta ccgccgtcag tttgggaagg
    27721 gtgattttga ttttcagatc ttcataatcg ccccaagtgt ttttggagaa tttgattttc
    27781 cactttttgt cattgacctc ggtctggatc aggtcgatga tgttggcctg gccttccacc
    27841 gtgatttgct gggtggctcc ctgggtgatt tccacatcgg cggaaatatt gctttcaatt
    27901 ttttcaaagg aagccagctc cagggtacgg gtgaccttcg ggcctgtgcc tttgattttc
    27961 tggctggagg ccgtttgtat tccggcaacg accaaaatca gcagaagaat aatgtttttc
    28021 atgatttcag aatttatttg tgctattaaa gacagcgtga attccacggg gttgcatacc
    28081 gtccgttagg gggacttcaa aatccggcga atccatccca tatccgatag gcatgaaagg
    28141 agcccgctct gccggcatcc gcagccctgc gatcaattat cctccatatc caaattgacc
    28201 atcttgatca ccgagaccat cgtttggccc tgggaaaaac tgtgtttcaa ttcggtctga
    28261 agcaaatgca tgcaggcatc aaaatcaccg gtaatctggg tgctcatggg cgtgttccga
    28321 acttcaacat tgcggtactg gctcaacctg gacagcaggt tgcggatagg cccgctgtag
    28381 tttttatcca gggggtataa actgagatca atggatatct tcatggtcat tggttttaaa
    28441 tcaaaaagac aaggctacca ttttaaccgg gtggataaat atacccaggc gagcccggcc
    28501 aggcaaacga taaacagggc cgactgcaga ttgatcgctt tagagataaa gccgataatg
    28561 accggacctc ccaggaaggt cgccacggaa aaggtgttca gggtggccag cccgacgcct
    28621 ttggccaggt tgggcagccg tgtggaggct ccgtacagga taggcgcacc gcaggaaaca
    28681 cccaggccca ccatgccgaa agccgcgatg cagatccagg tttttgggaa aaacacggca
    28741 accgcaatgg ccagcgcact tatccatccc ccatatttca gtatggaccg ggccccgtac
    28801 cggggtataa aatagtcccc ggaaaaacgg cccatggcca taaaaaaaga ataactggcc
    28861 agcccccatc cgatcaggta ctccggggcg tggaccacat ccctcagata caccgcggtc
    28921 cagtccgcca tggatccctc ggtgagattg gtgcacaaag ctatgccgat catgatcagg
    28981 aggggccccc tgggaaaagt gaatttgggt ttcaccggct cttcctccgc ggtgagttct
    29041 tcttcaattt gaaggatctc gcgacgcacc attagccaga tcaacagcat cagacccgag
    29101 atgatcatac tttgcaccag aggtggcgtt tttaacccga tcagcgtgga ggccatgagg
    29161 gagcccagca tcagtccgag gctgaacaaa ccatgacaag tggacatgat atgcagtttg
    29221 gaccggtgtt cgatgatgga ggcgcaggta ttcatggata cgttggtaaa cgcgatcatg
    29281 atcccggtga caaacaaacc aagccctacg ccccaggcca ccggagacaa gaccggaagc
    29341 aggtaacaca gggacatggc caccatggag gtcagggtca tcttctgcat gccataacgg
    29401 tggataaacc aggaggaagc cgggttggcc agcgccgctc ccacgggcat gcaaagaatg
    29461 atgagcccga gttgggcgtc gtccaaccca aattttgatt tgacaatggg gatcagggta
    29521 gcccagttgc caaacagggt acccatgacc aggaagaccg cgcctacggc cacgctctgc
    29581 cggtgcaata aaaacctgag taaatgtgaa aacatagaga gggggtaaag atagggattg
    29641 ggggattgag gattgaggat tgaggattga ggtttgagga ttgaggaggt cttctctact
    29701 tcttacctct tacctcttac ctcgtacctc atacctcata cctcatacct ttagtctact
    29761 cactacctta tgcaactgcc acacaaattc ctggatgagg ttttgcgagg tggactggcg
    29821 gatggctgtg gtataagggt tcatgggagt acagcggatg accaggaggc cttccgcgtt
    29881 ataatcttcc ctggccctgg aaatgcccgc atttgtaaaa aaatcagcca gggagtcaaa
    29941 aggataatac tgttcgtcca tgcgcgtgtg agatacaaaa aacgattggg cgtaactgtg
    30001 ttctttatca ccctgctcta cctgtatgga aaaatgcccg taaacggctc cggtgagcgc
    30061 attcatttcc cgcagacttt gatccgtttt gtttttcact gcaaagacca gcacattggt
    30121 atcaggaggt tcggggaagg tcaggatggt ataggtcaac ggttccggga accgggcggt
    30181 ctggtatatt tcttcccaat gcctgagcca ttcgtacaat tcccgcgtgg ccaggaggga
    30241 ggatttgatc agccggccgt gcccggaacg gttgagcggg atcatccggc ttgagaacca
    30301 tagggctgcg gctgccgctc caggcttgga accttccagt atgaacggcg cgaaggcatc
    30361 gatcccgatg tggtagtttt cgtaagggat gttgttggaa ttcaatttgg aataatccac
    30421 atcctttaca tgacggggtg ggtggtgcac caggatatcg tgttcggtgg acgtgatgta
    30481 aggggcgcgc tgcatgatga aatgccggat gcggtcattt ttaaaagcca ccaggccgca
    30541 gggatactgg atataaccca gtttatgcgg gtccaccgta atggaatccg cttttttaaa
    30601 ggagagaaag gctttgacga cctgaagatc gccgccccaa ctgatcctta tgggtttggt
    30661 ttgggccttg aggctgaaat caatcgtgtc cgaaacatgg cttgcaacat cccgcgcacg
    30721 gtcttccgcc ctgattttaa aatcctccgg gccgatgatc tcggaaagcg gctgcccgta
    30781 aggggacctg aggatggttc caaattcatc gatcagggct aggtattttt tcctgaaccc
    30841 gttaaactcg ttgcggcccg ctttttcttt taaccgcaga atcctttgtt ccacggaaat
    30901 caaaagaggt tcggcttcgt catcgggtgt cgcctggatc atggaccgtg tggtctccaa
    30961 caactgatag atctctgaaa ccaggccatt gtctgtatcg ggccctgcct caggatcaag
    31021 gaggcgattc agctttttta ccaggatttc cagtttctcc tcctggctca gtttgaaaag
    31081 gctccggata tatcctcccc atgccccgtc gatatgcaac caaaaagagg tattcatccg
    31141 ggcttctatt tgttcccgga gggcggcaat ttcatgcacg ggatccaccg ccccttcttc
    31201 cgtatttccc gcgatcgcga tcaccgctat ggggattttc ccttcttcca gggcggtcat
    31261 gattttcaat tcgaggtcct tgacatcaag ccggaaacgg ctgtcggtct tcaccaacac
    31321 gacattgttt ctcccgatcc ccaggacgtc cgccgctttt tttatggaat agtgggcggc
    31381 gccggacgca aagatcaccg ggggaaattc gctgaaaatc ctgcccagat gattggtcaa
    31441 gctgtaaggc gattccctga gatagaggtc cgattttttt acggcctccg cgatgtttat
    31501 tttttccgac ttgctgatcg cttcgatcag gcaggcaaaa agcccgatgg acgtttctgg
    31561 acttaggcca atggcttccc ggggactgag tgccttcagg tctgcgcgtt tgccattcgg
    31621 caaattcacc gcgatatcca ggcctttttc cctggcgatg tcatggacgg tcagcggaaa
    31681 atatttgatg atccgggcca cccacagcgc ttccacattc gccagggtgc caccggaagt
    31741 aatgtgggcc cagccgaatt ccgtcttcag tttttcttca taggcttgcc attctttgat
    31801 gccggcctct tttcccggag ggacgggggg agtgttgaag cccagcattt tcaggacctg
    31861 gttgctggct tcgatttccc attcgaccgt aaccggagcg ccttccgggg tgacgttgtt
    31921 ggcgttgtac agcatggccg cgaaataacc aaagaccgaa ggcatattga tgtccgacag
    31981 catatggccg atgtatcgcg ggctgtaaaa agggaaattg cgcctcagtt gagaagtcat
    32041 ttcagcaacc ccttgcatca atttatcaaa ctcttcctcg tgctgccgtt ggattctccg
    32101 cgtgatcaaa atcggatccg caggaaaata attccgcctc cagtggatat aatcccggat
    32161 gatggtcagg atggtgtttg tgaagatttc ttcattttca ccctttgggc cgagaaacca
    32221 ggaaagttgt ttcatggagg gaggaggtta gaggtaagag gtcagaggtc agaggtcaga
    32281 ggtaagaggt aagaggtaag aggtaagagg taagaggtaa gaggtaagag gtaagaggtn
    32341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cgaggtctaa cctctagcct
    32461 ctaacctcta acctctaact tctaacctca aacagctatc agtttgctgg tcagatcact
    32521 gatctcggcg tcggtgagtt catgatcttc cagtttacct gcgaaatagt ctccgtaggc
    32581 ctgcatatcg aaatggccat ggccgcaaag gttaaacaat atggttttgc gggtcccttc
    32641 ttcgcgggct ttggcggctt cctggaagac cgtggccagg gcatgggtgg cttcgggggc
    32701 cggaatgatg ccttcggccc gggcgaattt tacgccggcc tcaaaacatt ccagttgggc
    32761 atgggcacgg gcctcaatga gtttgtcttt taacaactgg gatacgatca cgccggctcc
    32821 atggtaccgg aggccgcctg catggatgga agcaggtaca aattcatgac cgagggtata
    32881 catgggcagg aggggggtca tgccgatggt atcgccaaaa tcataactga acacgccgcg
    32941 ggtgagtttg ggacaggaaa ggggttcgct ggcaatggcc cttattttct tgccctcttc
    33001 caggttcagg cggaggaagg gaaaggataa tcctgcaaaa ttggatccgc ccccgaaggg
    33061 tgcgacaatg atatcgggca gttcgccggc tttttccatc tggatcaatg cttcctggcc
    33121 gacaaccgtc tggtggagta acacgtgatt aagtacgctg cccagggcat atttggtatc
    33181 gggatcaggt gccgcccttt cgatggcctc tgagatggcg atgcccaggc tgcccgggga
    33241 gtgggggtcg gcggcgagaa tcttgcggcc ggattcggta tggtcggagg gggaagcgta
    33301 aactttggcg ccaaaggttt ccatcatgat ccggcggtaa ggtttttggt tgtaactcac
    33361 cttgaccata tagacttcgc attcgaggcc aaacatattg caggcaaaac tcagggcgct
    33421 gccccattgc ccggcaccgg tttcagtcac gatgcgtttg accccctgtt gtttgttgta
    33481 ataagcctgg ggaatggcgg tattgggttt gtgcgaaccg ctggggctta ccccttcgta
    33541 tttataataa atatgcgcag gcgtatccag ggccgcttcc aggccgtatg cccggtataa
    33601 aggggttggc cggtataatt tataggcctc gcgcacggca tcggggatct ccacgtattt
    33661 ttcccccgtc acttcctgtt tgatcagttc catggggaag aggggttcca gcatttccgg
    33721 cccgatgggt tgccgggtac ccggatggag gggaggaagg ggtttgttgg gcatgctggc
    33781 aatgatattg taccagtggc ttgggatttc tttttcggtg aggaggatct ttttttccat
    33841 atgaaaatgt tttacgattt agtcttgaat ttccgggata agaggtcctt tgcccggttg
    33901 atcattttac cggtaaaata atcacaaagg ccgagattgt tttcgatctc aaaccaggaa
    33961 ctgatttctt tttcggagaa aagatggcgc agcgactcct cttttagaag cagggcctgg
    34021 agggattcac cggtggatct taccctggta ctgaaagaac ggaccagggc ctgggcttcc
    34081 tccgtattta acttaaggct taacatccgg ctcacgtgct ctgcaaaaac cagccctccg
    34141 ccgtcatcga tgttttttag catgcgttct ttattcaccc ggatttcccc gatcatttct
    34201 atggaaagac gaagggaacc ggccaccagc ctgaaaagat cttccatgct ttcccactcg
    34261 gcatgccagg ggccggagcc tctttcatga tcctgggtca gggtattgga aagggaatat
    34321 gctatggctt gtatccggag gccattggcc aggatcagga tggcgttgac cgggttggtt
    34381 ttgtggggca tgatcgatga atggcccttg tcttttccgt caaaactgat ttcaccgact
    34441 tcattttggg ccaggaggct taaatccgta gccattttgt tgaggtgacc ctgcaaggcc
    34501 ccgagctggt gtgccatagt atggatatac tgtctttggt tatgccaggg tttgacgggc
    34561 aggtccaggc ccagttcccg ggacaggccg gaggatacct cctcatattg ttttacgctg
    34621 taccccgggg cagtcccgac ggatccaccg agttgaaggg gaaatttaag aaaccggagg
    34681 ccttccgata ccatgagcag cccatcgatc caaccggcca ccttaaaacc gaaggtaata
    34741 ggccgggcat gctgcataaa cgttcttccc gccatggggg tgtggcggtg catttcggcg
    34801 atttcgacca ggccggtgat cagtggtctg aggttcaggt tgatccaggc cgtcgctttt
    34861 ttatattgga tcatcgcggc cgtgtcgatc gcgtcctggc tggtagccag gaaatgcaga
    34921 tagccggcgg cttcagaggg atggccggcc aataactgcc tgcattggcc gagcaggggg
    34981 atcaggatat tcccgctgcg ggccgattct tccatcagtc tttcccggtt tatggattca
    35041 tagctgcaca ggatttcaat agcgccggcg gcaggaggag gaatcagccc ttgcctggac
    35101 tgtacccggg ccagcgcttt ttcgaaacca agcaaggcct ccaggaccgg gcgaacttca
    35161 aaaaattcaa aggcccggtc gtaaaaaaga ttgctatata atgaaggcat tcggcaggat
    35221 cgacagctaa aattaagcca gaaattgatg cagccggtca ggggtttttg tttctacatc
    35281 taaaagctaa gaccggatga tcctgtgtgc ggtcaaatcc gataatttcg caacatcaat
    35341 ggtatattcc atggttttgg ttaaacttta aaattgatcc tatttgtcta ttatggaggt
    35401 gtttaagaat tcaacgggcg cctttgctcc acagaacagc agcaccatga gatactgcaa
    35461 agactttatc ctttgcgccc tggccctttt atgtatggtg aacctggcgg gcgctcaaaa
    35521 attctggttg acgacctatg aatttcccgg agggcccaaa accgggataa gcctcgtgca
    35581 ggacagtgtt ttatttgttt caaccctgaa ctacgtgatg aggagtttta atgaggggaa
    35641 taaatttgac accaccctga gggcggagag cctctccacc gtatttgcct cccgcaccgg
    35701 gacggttttt gccggcggga agggcaaagt atattttacc agggacttcg gaacaagctg
    35761 ggactccgtt ttggtcaata cggccgccca tatcacagga tttgtggaaa atacagcgag
    35821 ggaccctttt gccatgaccc gtatttatga cgaggaaaag gggtatatcg gggacggggt
    35881 gcttttttcc aataccgggg gagccagctg ggcttttcga aacagcggcc ttgggtcgca
    35941 gaaagcgtgc gaacatctgg tcatagataa aaacaaccgc ctttatttag ccattgggga
    36001 tgaagcccgg accggcatgg gcggattgtt tgtgtcggaa aacaatggga tgtcctggca
    36061 aggcatcgcc ctcccctttg acgggaaaaa tcatgtgccg gatcaggtca ttcccaccac
    36121 catcaccggt ttatcggttt cgccccagga cagcttatat ctcagtctca gcggggtagc
    36181 ggtcaatgta ggcgtcagcc ttaatatcta caaaagcatt gacgacgtca caaaaaatac
    36241 attctggaat atcatgaatg tcattccaga taatcagttc acggaccgga atctgaattc
    36301 catccacttt gccggaaatg gggactggta cagttccttc agcgggaccc tcaatgtggg
    36361 aggaacctat tattccaaaa acaggggcgt ctcctggcag aggatcgatt tcggacttgg
    36421 cctggatatt ttcggaatgc gtaacgtgca gtatttcgtt gaaaaacaga acggcaagat
    36481 ctttatggtt cactatctga atgaactggt gcactacacg gatacgagtc tgacgacggc
    36541 aaccggggat gtccggatac gaaataataa attaactgtt ttcccaaacc ctgtagcttc
    36601 cggggagaat ataaaaattg aattgcctgc ttccatgcaa aagtgttcca ttgccgtatt
    36661 gaacagctca gggcaggtgg ttctggcgaa acgggtaaat cagtcatccc gcgtcgaagt
    36721 cgacatgacg gttgttcctg gtgtttattt tatccgggcg atagagggga atgtggcggc
    36781 tgtgcaaaaa gtgttggtgt tttaattccg gggcggactg gattcctttg acgcttcgcg
    36841 gtttgcccgc caggggatcc cacgaagtgt gcaggagttg ggtgaactat aacgtcagaa
    36901 gagataatat aagtaaaagg ctgggcataa gaagcctcct tcgggaataa aaatttaaat
    36961 atctccaccg aagtcttata tttttaaatt actgattacg aattgtttgg catttagtta
    37021 tgtttgagac accaagggca caaaagattt cacacaatta tacagcgcac cgaagaacaa
    37081 ggatttaatt cttccggcat cggagatgcc tgaccttatt ggtaaaaaat aagaacaaaa
    37141 cattggcttc ggagaagctc aacaatcgca gtcccaaatt aaaatagtat tgtttttttt
    37201 cgatattgga tatggtgacg gctatccttt gggtattggg tcggggggat aataacgttg
    37261 atgtttccag atgttgggct tcttcgaagc cggggtttta ctttggatga gtattaaaaa
    37321 cgtggcgcat cttcgatgct tgagatcttt gcttgaatag gttccttaca aagacactat
    37381 ggggatccca cgaaatgtgc agcaattggg tccactgcaa catcaagagg gataactttg
    37441 gtaaaaggcc tggcataaga agcctttttc gtgattggaa atttaaattt ctccaccgaa
    37501 gtcttcttct gattacgaat tgtttggcat ttagttatat gggagacacc aagggcacaa
    37561 aagatttcac acaattatac agcgcaccga agaacaagga tttaattctt ccggcatcgg
    37621 agatgcctga ccttattggt aaaaaataag aacaaaacat tggcttcgga gaagctcaac
    37681 aatcgcagtc ccaaattaaa atagtattgt tttttttcga tattggatat ggtgacggct
    37741 atcctttggg tattgggtcg gggggataat aacgttgatg tttccagatg ttgggcttct
    37801 tcgaagccgg ggttttactt tggatgagta ttaaaaacgt ggcgcatctt cgatgcttga
    37861 gatctttgct tgaataggtt ccttacaaag acactatggg gatcccacga aatgtgcagc
    37921 aattgggtcc actgcaacat caagagggat aactttggta aaaggcctgg cataagaagc
    37981 ctttttcgtg attggaaatt taaatgtctc caccgaagtc ttctttttta aaatttcaga
    38041 ttacgaattg tttgtcattt agttatctag gagacacgca gttcacgaag aaatggattc
    38101 attatgataa tcaaataaaa ttaaatttat gcgctccttc atatcgcagg gtgagctcct
    38161 ctcgtgtgat cctgtatcgg tgcacctcag ctgatctttt agaatctgat ctcaataaaa
    38221 cgctccgcct cccaaaggat tgaaatattc agcgcgctac tgaaacctct tgccctcaga
    38281 ccttctccag taataccgcg taaccctggc cgaccccaac gcacatagtc accagggcat
    38341 attttttatg taaaagatga aggtcgaggg ccgcactacc cagtatccgc gcgccggtca
    38401 tgcccagcgg gtggccgagg gcaagggctc ccccgttgac attgatcctg gggtcgcccg
    38461 gatccagtcc cagttcgcga aggcaggcga ggacctggac ggcaaacgct tcgttgatct
    38521 cgatgagatc catgtcgtcg agtttaagtt tggttttctc caggacccgg atcgtcgccg
    38581 gcacagggcc aatgcccatg atccggggct cgacaccgac cacagcggag cctgcgattc
    38641 gtgccagtgg tttgaggcca tattgaacaa tggcatcggc ggaggcgacc aggagagccg
    38701 cagcgccgtc gttaagtccg ctggaattgc cggcggtgac ggttccttcc ggtttaaaag
    38761 cgggtttgag gcgggccagt ccttccgggc tggtagccgg tttgataaat tcatcgcggc
    38821 tgaacaggct gacgttttgt ttaggatcgg tgatttcaat gggaatgatt tctttttcca
    38881 tccggccgga agcgacggcc tttgcggctt tctcctggga tccgcaagcg aacaaatcct
    38941 gctcttcccg gtatatccgg tattttgcgg ccaggttttc cgcggtgatc cccatggaat
    39001 cggtcccata catctgcttc atccgtggat tgacaaagcg ccatccgaaa ctgctgtcat
    39061 gcatctgggc gtccgtgcca aaagctttgg atgtttttga aatgacccag ggtccccggg
    39121 tcatgttttc gaccccaccc gcaatgatga gatgggcttc gccgcattga atggcgcggc
    39181 cggcgttgat cgtggcggcc atgccggacg cgcacaggcg gttgacggtc tccccgggca
    39241 cggtgaacgg gagtcctgcc aggagtaagg ccatgcgtgc cacattgcgg ttgtcctctc
    39301 ccgcctggtt ggtgcacccc aggatcacat cgtcgatttg accgggcgga aggtccggat
    39361 ttttttccat cagctttttg atcacccagg ccgcgaggtc atcggtacgc agggtggaca
    39421 aacctccccc gaaattgcca atcgggctgc ggacataatc gacaaggtaa gcctggtcca
    39481 ctacttgctc agttttttga tcggggtgag caggagtttc cggaattcga cttccgaacc
    39541 ttctgcctgg agggcaaaac tgccgtggtc tgcggtagca ttaaaaccgt gattgaccag
    39601 gtccccgttg acccagactt tgatttcatt tttaaggcat tcgatgttca tggaattcca
    39661 ttggccggga ggtttttcgg aaccgtcggt cagattgggg atcctcctgt ttttatcacc
    39721 gtcgatcccc caattttcct tcggtccgcg ccgggcttcc atatcaggca cggtgatgtc
    39781 ttccccgatg caccagaaat cgcccgcgtt ttcatgcatc atttgcactt ccaccgattg
    39841 tggaaacatg ccgtacaatc tccggggttt ggaagcatgc accagggcgc cgcaattgcc
    39901 gggctttccc gcaaaacggt attcaatttc cacccggtaa tcggaaaaag accggtccgt
    39961 gatcaaatgt cctccgggtg tgcccaggct caccaggtat ccattcctca ccacgaaggg
    40021 attgatggca ttgggatttt tgtccatttc aggcacatcg atatgccagc ccgtcagatc
    40081 ctttccatta aaaaggggga tgcttttttt cccggtcgtc ccccgggatg cagtacaact
    40141 gtaaatcaag ggcaatacca gaagaaaata tattaatcgc attgtcatat gaaatttttg
    40201 agtattcagg tttcagctat gagcattcag ttgactactg actactgact actgactact
    40261 gactactgac tactgactac tgacta
//
LOCUS       DAOQXT010000036        39501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1171_length_39501_cov_5.842975, whole genome shotgun sequence.
ACCESSION   DAOQXT010000036 DAOQXT010000000
VERSION     DAOQXT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 39501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39501
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_39501_cov_5.842975"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1923)
                     /locus_tag="PKM27_08220"
     CDS             complement(<1..1923)
                     /locus_tag="PKM27_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNR07285.1"
                     /translation="MNRDALLLEAGPETGIAGDTMEARILKDYWISLVSREVSILGRK
                     DVLSGKGKFGIFGDGKEIPQVALSHYIQPGDFRSGYYRDQTIMFSLGLCSVYEFFGQM
                     YSDSDNDPFSGGRQMNNHFATPLIGEDGNWTAHRDKYNVISDVSCVAGQVGRALGLAQ
                     ASCIYKSRADLDGTPFSNKGTEIVFCTIGDGGTAEGPFWETMNAAAIMQVPLVMIVWD
                     DGYGISVPVHLQTVKASISKALEGFLGESQGEGIQIFTVKGWDYPELCKVFEKATQIS
                     RLYSTPVLVHVQEMTQPQGHSTSGSHERYKSKDRLDWEKKFDGISVMRSWLLDAGLLS
                     LQEDEALKIKAKEFVKKERDRALAHAFDHTRKELELVVDLLAEVAASRPYHAGRVQHH
                     IDELRTLREPVSSDLARSIRQLLIATPDQEKDPVWTKLKNWLNEVYQKAHLRFHTHQT
                     SETRNAAIRVKPIPPVYSDHAPTLSGYKLLNLYFDKLFESNPRVIAFGEDVGKIGDVN
                     QGFAGLQMKYGEERIFDTGIREWTIVGQAIGMAMRGLRPIAEIQYLDYLIYALSPLSD
                     DLACLRYRSNGVQCAPLIIRTRGHRLEGIWHSGSPMGMMLNSLRGIHILVPRNMVQAA
                     GFYQTMMQSDDPAIIIE"
     gene            2137..2607
                     /locus_tag="PKM27_08225"
     CDS             2137..2607
                     /locus_tag="PKM27_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNR07286.1"
                     /translation="MKKLLSLSTLCLLAGIAFAQNNAPAEVKTGPVMTLSSDVVDYGE
                     IMQNADPVRKVKFTNTGTEPLIIKNAQGSCGCTVPLWPKEPIMPGESSEIEIHYATDR
                     IGQIMKTVTITTNETGVNHVLQVKGNVKPKPVDETVPTKQATLLTPVKKQNSNQ"
     gene            complement(3086..4555)
                     /locus_tag="PKM27_08230"
     CDS             complement(3086..4555)
                     /locus_tag="PKM27_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR07287.1"
                     /translation="MKNVVYLFILLTGTSTLFGQDTLSTLSQTPPAQAGSSVNGISFK
                     RAFIDYKSPNAGSLSNFGSFREFNSGFEVGYLRNFTDNLRLYIPARIGVFSVNDQSEN
                     ITFGELDAQLQYRFNSKKGTLRPYLLLGAGVNTENLDTFNVQLPMGAGLDVKIARSTY
                     INFQAEFRLSLSDQRNNNQFSVGFIHYFGKGAAKKEEKAVSEAKDSDGDGIPDDLDLC
                     PNKPGLKQFAGCPDTDGDGIEDPNDLCPEIPGVKALRGCPDSDGDGVPDKDDDCPNLA
                     GTKLNRGCPSTDTDNDGVIDDLDECPTVAGSPSARGCPDKDGDGVADRVDKCPNVAGK
                     SKNGCPDTDGDGVDDGEDRCPNTFGPASNRGCPEIKQEDKEVLNIAMRDVQFQHNSAT
                     LTNDSYRILDQISQILRNYPEYKIEISGHTDNTGSADFNKRLSEQRARACRDYLISKG
                     LAWARVSSRGFGQSRPIATNETQQGRATNRRVEFKLYLD"
     gene            complement(4723..5217)
                     /locus_tag="PKM27_08235"
     CDS             complement(4723..5217)
                     /locus_tag="PKM27_08235"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNR07288.1"
                     /translation="METIQVFVTGGTFDKEYNLITGELYFKDTHLKEMFARGRCKLDI
                     DIKTLMMVDSLDLTDADRSIIIHNCKRSPHRRIILTHGTDRMVATATEIARAGIEGKT
                     IILTGAMIPYAFGNSSDGFFNLGAALAFVQTLSPGVYLAMNGRYFDWDKVRKDMDTGF
                     FEEA"
     gene            5279..6634
                     /locus_tag="PKM27_08240"
     CDS             5279..6634
                     /locus_tag="PKM27_08240"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNR07289.1"
                     /translation="MLYLHRSFWLMFFMMRAEFGLFAQDPEGLNPMQGVVDVQQYDLE
                     LWIQPGDTTIRGKTTIDLQIVKTTRELILQFEDLRINALTSNSGKIKYTYDGKQIMIR
                     TKKKLKPGRSIRLVIEYEGKPSDGLIIRQNEGRYTVFADNWASRARNWFPSVDHPSDK
                     ARFHATVHTADPYTVIANGIKVGESGLNGKKSFVYHMDHPIPTYCMVIGLAKMSIAGD
                     RTKGGIPLFYYTYPEDSAPARESFKRVADMVHFYDSLIGKYPYDRLSLVQSSTRFGGM
                     ENSSAIFLADQGAVYRRRSDPEGLLAHEIAHQWFGDAVTQSSWADLWLSEGFATYFSA
                     LYFEARDGKNKFDSIMDRSRNEYLRRSPRKAPVIYDGYPQLFQLMNAENYNKGGLFLH
                     ALRQAAGDSAFFSAIRKYYAQFKHRNATTADFQALAEAESRKDLDELFTLWLRRPGLP
                     E"
     gene            complement(6702..7421)
                     /locus_tag="PKM27_08245"
     CDS             complement(6702..7421)
                     /locus_tag="PKM27_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR07290.1"
                     /translation="MVKTNNKILLVEDDQNFGDVLRSYLEMHDFEVTLATDGQEGYEA
                     FRKDHFDLCIFDVMMPKKDGFTLAKEVRQKDPEMPIVFLTAKTLKQDILEGFKTGADD
                     YISKPFNSEELLYRIQAILKRSKKAADPREEIKEFTIGKYHFNYPLRLLVNTVTNKKD
                     KLSPKEAHLLRMFCMYKNDVLARSEALTKIWGEDNYFTARSMDVFVTKLRKYLKDDPD
                     LEIVNIHGNGFQLLVKNEAPV"
     gene            complement(7617..8972)
                     /locus_tag="PKM27_08250"
     CDS             complement(7617..8972)
                     /locus_tag="PKM27_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR07291.1"
                     /translation="MTKRTIWLIIISMGLALLGSILLQMYWIRLTLREQENQFDREVN
                     QALVGVIDEVERRDNRNLEPLADLPPAESQKLITEYMNKGIIERLDGRAGFLFIDSLL
                     KRAVHKLGNRPEYHYGIYSNRDSGFVVVDNFFTVTSLKSSLSRTGIKSGLYYTPYKIN
                     IFDRSSQGSGRLFLYLPHRNQIIWKNLIPAAVGSLLFTSLILVCFAYTIYIIQRQKKI
                     SDMKNDFINNMTHEFKTPIATISLATDSINNPSIISNQDKVQRFTRIIKQENARMLGQ
                     VEKVLQLAQLEKRDLQLKLSEINIHELLQAISENVNLQIKGKKGDLKMNLEAKDPVIE
                     GDLTHISNILYNLLDNANKYSPDSPDIRVATRNIHHGIEISIEDKGIGISRENQKYIF
                     EKFYRVSTGNLHDVKGFGLGLSYVKVMVDAHQGHIQVKSALGKGSNFILYFPKHQNKN
                     L"
     gene            complement(9071..16222)
                     /gene="sprA"
                     /locus_tag="PKM27_08255"
     CDS             complement(9071..16222)
                     /gene="sprA"
                     /locus_tag="PKM27_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNR07292.1"
                     /translation="MKSFLLGFCSIWIVSGFANSSKATLPPNPGNIKESLTHIPDTTP
                     AQKIRLPQDKKDPIDLRDPASIEKEVQYDPETNRYILTERIGSEYLSTQSMTFEEYVR
                     YKNKQLQQDYFNELAGSSSGRQTTGRDPVEKIDVKKTLIDRLFGGNTVEIRPQGNVDL
                     TFGANYQNVKNPSLTIRQQRQTNFDFDMNIQMNLQGKIGEKLNLNTSYNSQATFDFDN
                     VMKLNYNSDQFSEDEIIKKIDAGDVSLPLRTSLIQGAQKLFGVKAETQFGHLRLTGIA
                     SQSRTQQQRINLQGGALLQDFEIPGDEYDENQHYLLGHYFRNTFEGAMVRLPYISTLA
                     KIDLNSIQVWVSPDPRDINNLRQVVAFSDLGEYDRMNNPDPDFWRMGSTPVIDMTTNQ
                     KLPTNGTNRIYQQFINDPSLRQTDKVVSGLQRPPFNFAIGRDFEVFKGKLLDQREYTV
                     HPDLGFISFRRVVRPSDIVAISYKYTFNGKAYKVGEFTQEVAPDSLNVIVTKLVKPRS
                     NRVDIPIWDLMMKNFYSLRSFGISEEDFKLDVLFDDPGAGEKRFLPETELKSIPLLNL
                     LNLDKLNKQRDPFPDGVFDFVPGLTIDPQSGRMMFPVLEPFGESLGKKISDPDLRKKY
                     VFQQLYDSTKAASLNFPEANRYIIRGEYKSSSGGDVALNSFNIPQGSVRVRAGAQELS
                     PNQYEIDYQTGRVRVTDPAILSSGVPVSIDYEDNSLFGFNNNRTMFGLRGEYEFNKHL
                     SVGGTVMRLFERPYTQKVNIGEDPINNGIYGLDLVYNNEAPWITRLLDRLPLLSTKEN
                     SKISFLGEAAALRPGHSRAINQGKDKGGTAYIDDFEGSTSSQDLGIPVNQWFPASTPS
                     GRRFRESAPQNSNTTFPLVNRAKMSWYRIDRRVRGGNEDAYSTAINITELFPNRNIGP
                     GQNIELYTFNLNFNPHQRGPYNFDVPGGTPYSAGLDQNGNLLEPRSRWGGIQRGLYNT
                     DFEAANIETIEFWVLNPFMSKSDGSPVTTGGNLYINLGDISEDIMKDGLMYFENGLPT
                     TKNMTRIDSTAWGRIPLVQAITRAHNNDPEILKQQDLGFDGLDDDGERRYYADYLQKV
                     QSLNPLVYERIREDPANDNYKYFNDESFTNTDNIYTRYKDWNNPQGNSSFTEQSGTEA
                     KNYTQIPESEDINDDNAFDQSEAYYEYRVPMYPDNAGGMAINKYITDTISGPGGRIWY
                     RVKIPLESPDTTVGSISGLRNIRFIRMYMADFDQSVTLRMNSIDMVRNQWRRYRLPLC
                     ASDDIDNSFKFDVNDVNIEENSQRFPIPYVVPAGIQRENSIGPFPNLLQNEQSISLSV
                     KELKGGCGCAVYKPIRMDMRNYERIRMFVHADSPEDLGTEEFTTFLRIGSDFTSNYYE
                     YRIPTRLSKRDQVIPGRESTEIWKVENELDLSLQALVELKKERNANNAPLDLEYAIPD
                     PENPERIIAIVGNPTLGKVKQVMLGMRFPVRSARQHEADVWFNELRLNGLNEQGGYGA
                     IGRLDIQLADLGDLSISGNMNSIGWGALDQKVLERRQEDLKQFDINSNLELGKFLPSK
                     WGIKIPFYTQLSVNASAPKFDPYDLDIKLKDKLLETRSSSEKDSIRRQAQAYESIRSF
                     NFTNVRKERTGDSKPAPWDISNFNFNYSFTQTTNHDPIVEKDQENVYKGGFNYNFARQ
                     AKYLTPFRKLVKNDKLVRWFTQFNINPLPNSFSFSTGLDRRYGTRAFRFSTPEYGRAI
                     TKLFFWDRKYNLQWDLTQSFKFSFNASNNSAIDELAVMDSLGRLDPTYDPKRNKEVIW
                     DNIRSLGRPKNYMHNYNASYNLPTRHIPLLDWVQVTAQGTGTFAWLAGSENVVDFLGN
                     VIQNSQTRQLSANLNFDALYDKWAYLQKINQPPSGQGQPARTRDRNAPADQSGQPARN
                     TRERAEKQPSWIERALIRPLMSLRRGRLTYNENLASLVPGFRSLPKYLGLANGFGEPG
                     WAYILGMDPSQTWLEDIAQKGMITDDIRLNQQFSRNYNQTLDARLTLEPFNDFSIEFS
                     LNKNYTRNYLEFFKNDGLGGGFKHLTPTEVGSFNISYLGINTLFGKNVDSLYAQFERN
                     RAIFSERQPNKPDAGDHPLNEGYKEGFGRKNQQVIIPAFIATYTGLDPQSSGLDVFKT
                     NPRPNWQLTYNGLHKLPFLKNFLSGASIRHGYTSRLMVNQFNSDVFYDPDSAYRTKPL
                     TADYYSRIEIPNVVMTETFNPLIGIDIKTLSDLQLNFDYTIQRSLQLNMIDYQLIEQN
                     SKEITVGFGWRVKNVVMPFGKKAPQRTRPNAPTDNQTPDARGRTSNQAKKGSDLNLKF
                     DFSFRDDLTNNRVLDQDQNVPTRGAKMLRIANAADYEVNDRVTLRLFFDYSRIIPAVQ
                     ESFPITTAKGGVTVRLALK"
     gene            complement(16378..16971)
                     /gene="ruvA"
                     /locus_tag="PKM27_08260"
     CDS             complement(16378..16971)
                     /gene="ruvA"
                     /locus_tag="PKM27_08260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNR07293.1"
                     /translation="MYYYLKGKLSVIHPTLAVVDCHGVGYHVHISLHTYSQIDKKEEV
                     LLYTHLAVKEDAHVLYGFAQLDEKQLFLQLISVNGVGTGTAQLILSALTPDAIKSAIL
                     REDDLTFKAIKGIGEKTAKRILLDLKDKIGREPESKSVPAGPVYHNTLQAESLSALIA
                     LGFQRQKSQQVIAGILQKQAIQSVEELIKLALKQMSS"
     gene            17162..18160
                     /locus_tag="PKM27_08265"
     CDS             17162..18160
                     /locus_tag="PKM27_08265"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF010578.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HNR07294.1"
                     /translation="MRHSFFCIDAHTCGNPVRLVAGGAPMLSGNNMSEKRQHFLREYD
                     WIRQGLMFEPRGHDMMSGSILYPPHDPANDFALLFIETSGCLPMCGHGTIGTITIAIE
                     HGLIRPKTPGKLSLEAPAGLVLIEYRQEGDKVESVKLTNVPSFLEAAGVEVECPGLGL
                     LHVDIAYGGNYYAIVDEQANYKGLDHYTAGQLIQWSPVLRQRINEKLKLVHPGDPTIG
                     KCSHILWTGRPRSEHAHARNAVFYGDKAIDRSPCGTGTSARMAQWAAQGKLQTGDSFV
                     HESIIFSTFTGKVEGTMDYYGRQAIIPSIEGWAKVTGYNTIFIDDDDPYAHGFQVI"
     gene            18170..19378
                     /locus_tag="PKM27_08270"
     CDS             18170..19378
                     /locus_tag="PKM27_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR07295.1"
                     /translation="MRVCVIGGGVIGLFSAWYLVRSGHEVCLVDRNNFNEGASHGNAG
                     MICPSHFTPLAAPGVIRQSLKWMFDSGSPFYIKPRFDPDFIRWGLQFFRKANRRHVKA
                     SIGPLSALLQWSSSLYRDLAEEGMDLSLSQRGIIMACKGQHTLDEESHIAGQAAGIGI
                     RTRILSSGEIHDLNPGVRFKLTGGVHYLDDMHLVPEKLITSLKADLSGKAEMLSGIEV
                     TGFHRENDRIDRALTQAGEIRADFFVLAGGAWTGKLARMAGEYAAIEPGKGYNITIPE
                     ANPQLSTPLILVEDRVAITPMDQALRLGGTMELSGINDLVLTRRVTGILRAIESCLPD
                     FGMDQTSVLKPWFGFRPLSPTGLPVIAPLGRLRNVMINAGHGMLGLSLAPASGKLLNQ
                     LIADHVRGVA"
     gene            19375..21192
                     /gene="uvrC"
                     /locus_tag="PKM27_08275"
     CDS             19375..21192
                     /gene="uvrC"
                     /locus_tag="PKM27_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNR07296.1"
                     /translation="MNREAFKLVQPTIPELPGIYKFLDEKGTILYVGKAKNLLKRTSS
                     YFLDSKNHSFKTVTMVRHAVRLEFLVVETEHDALLLESTLIKKNQPRYNVSLKDGRSF
                     AFICIKKENFPRIFFTRRVFRDGSEYFGPYTSKYKAEIVLDFVKSLFPLRNCTLNLSP
                     ENIKNHKFKVCLEYHVKNCLGPCEGFESKADYDDRIRQIRNILNGNFSEVTAYLQEQM
                     ELCASNLDFESAQACKIRLDAFNDYQSKSMVVHPSIGDVDVFSIELEDHEGTVNYLRI
                     IKGALINSHTFELDKNLDQDPEMILAYAILECRELFSSIADEIIVPYPVDLPGERLKI
                     TVPQRGDKKKLLELSQKNLRYYILAKKSEIATHTTKPVAVQRILNTLRIDLRMEKDPA
                     HIECFDNSNIQGSHPVAACVVFKNAKPAKNEYRHYHVKTVEGPNDFASMSEIVYRRYK
                     RVLDENKPLPDLIVIDGGKGQLHAAMESIIRLGLENKVKVVGIAKRLEEIYFPNDPVP
                     LYINKKSESLKVIQKIRNEAHRFAILFHRDQRSRSLLSTGLTDIPGIAKKSAETLLKH
                     FGSMARVQEAGLEEMAPLIGRKKADLVYRYFRPEAEEEE"
     gene            21280..22356
                     /locus_tag="PKM27_08280"
     CDS             21280..22356
                     /locus_tag="PKM27_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNR07297.1"
                     /translation="MLTVGIISSKGDPRIALVPETVDKYLKLPCTLLFEKGCGQSASI
                     PDEVFGTKVVFEPRTEVLSKSDILLSPLPPERDDLQHIRPGTVLVSMFAPFQNPGVAD
                     PLKEKQVSAYSLDMIPRSTLAQAMDVLSSMASIAGYKAVLLAASLLPRYFPMLMTASG
                     TIKPAKALVLGAGVAGLQAIATARRLGAVVEVFDTRKAVKEEVESLGAKFVEVEGAKD
                     EKSAGGYAVEQTEEFLAKQRQMVQERAAKSDVIICTAQVRGRKAPVLITSDTVAKMRP
                     GSVIVDLASSTGGNCEVTRDKQTINHLGITIIGNSQLADTVAIDASFLLSNNYLNFLK
                     MLVKEGKINHDAGNEILRSSCITL"
     gene            22371..22676
                     /locus_tag="PKM27_08285"
     CDS             22371..22676
                     /locus_tag="PKM27_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNR07298.1"
                     /translation="MLDWIQNHQQIIYIVLLMVFLGIEVIGRVPSVLHTPLMSGANAI
                     HGVVIIGAIIVMGKAEPDNYLALLLGFLAVILGALNVAGGFVVTDRMLEMFKQKKSK"
     gene            22676..24094
                     /locus_tag="PKM27_08290"
     CDS             22676..24094
                     /locus_tag="PKM27_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNR07299.1"
                     /translation="MELSLLTIIYLVAAVTFILGLKMLSSPASARKGNLIAAFGMVLA
                     ILGTIFFYRDEEGKVLHNYLWIFGGLAIGSGAGWVAARKVKMTAMPELVSLFNGMGGA
                     CAALISLIEFNHLSHSAGNLSAVSGGTLLIIFLGLIIGSISFAGSVVAWGKLNGRIKD
                     FSFKGQHWINFIILALVVFLVAFILISRPETSGMLFYAVLFLSLLYGFFFVMPIGGAD
                     MPVVISLLNSFTGVAAASGGFLYDNKAMLTGGILVGAAGTLLTILMCKAMNRSIFNVL
                     VGNFGGGAAAATGAAAGGARAVKEITASDLAINLNYSKSVVVVPGYGLAVAQAQKTVK
                     ELEDVLEANGVEVRYAIHPVAGRMPGHMNVLLAEANVPYPKLVDLEEANEMLAQADVV
                     LVLGANDVVNPAAKDDPTSPIYGMPVLEVWNAKLVVVVKRSMGAGYAGIENPLFFHDR
                     TKMLFGDAKKVLQDLVNEVKAL"
     gene            complement(24387..24824)
                     /locus_tag="PKM27_08295"
     CDS             complement(24387..24824)
                     /locus_tag="PKM27_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07300.1"
                     /translation="MLLLTVSGLQIHPLNSQPAPNRAPGIGSATVIHKEAGPEADRWE
                     ITFHDALNKNRVLWILVKTDRGKSFLPGSYRFRKKPFSAFGKRDILGVVYQDKKETPV
                     RKGRVKLAISGQELSLHARLRLKNSGTLSYGYEGRFRIIELKE"
     gene            complement(24950..25654)
                     /locus_tag="PKM27_08300"
     CDS             complement(24950..25654)
                     /locus_tag="PKM27_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07301.1"
                     /translation="MKNSILLFLVLLLALPVLGQSDAKALEVIQNSIAKSGGKEKLSA
                     IKTMTRKMEINMPFGISESEAWYKDGKFYMKSTMQGNVMFEQKYDGTRAFIGGMQGNQ
                     TIDDEKLIKQIANQGKIFPQLSIINGEVTLKHDGMEKIEGEDCHRILAVDADGTTSKM
                     YFNASTGLLSRMATTGEFQGTKVETVIDMKDYKPVDGILFAHKMQLNNGQFQMEMTVT
                     ELKFNVDIPDSMFFIN"
     gene            complement(25673..26458)
                     /locus_tag="PKM27_08305"
     CDS             complement(25673..26458)
                     /locus_tag="PKM27_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07302.1"
                     /translation="MKNAFYLFLFIFALGMVSCSKKTATAVQAPPEKSDTPVAAKTNE
                     SDQKAIDVLKNYINAIGGSDKLTSVKSVMMKMSASTGMGDLLITQFIKDGKMAMKTEM
                     AGSTVMEQKFDGTTLQVSGMGGNQKITDEQTLKGVRKQARMFDELDQLTSSKSLKKYL
                     GTEELDGKTVHKITVTDEDKNESTQYFDAASHLLLRTIATVDAMGQKSTQTMDFGDYK
                     EVNGVKFPHQVKMTGGAVPFPLDMKITALMINSDLPDQLFLIN"
     gene            complement(26542..27387)
                     /gene="pyrF"
                     /locus_tag="PKM27_08310"
     CDS             complement(26542..27387)
                     /gene="pyrF"
                     /locus_tag="PKM27_08310"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNR07303.1"
                     /translation="MNTPFWDRQALNRAIRELQSCLCIGLDTDETRIPRAVTGKDKVF
                     DFNKSIIDATADLAVAYKINAAFYEAMGQAGWQAMADTIAYIRDKRRFVILDAKRGDI
                     GNTGELYARACFEQLDADAVTVSPYMGRDSVLPFLSRPGRWAILLALTSNTGAEDFQL
                     QRMASGRKLFEEVIHLSSRWGSPDQLMYVAGATRIGNLLEVRNLLPEYFLLIPGVGAQ
                     GGDLGQVMKQAMTTSGDLLINASRSILYASAGPDYIQAAREEALKMQSSMRTHILEKS
                     VNLEP"
     gene            complement(27387..27641)
                     /locus_tag="PKM27_08315"
     CDS             complement(27387..27641)
                     /locus_tag="PKM27_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNR07304.1"
                     /translation="MNLTRKSKEKKIQEIDQALDELRPHLAVDGGDVEVIDFTEDLTV
                     KIKWLGNCQSCSMSAFTLRAGIEQAIKSKFPEVSAVIAVS"
     gene            complement(27683..28768)
                     /locus_tag="PKM27_08320"
     CDS             complement(27683..28768)
                     /locus_tag="PKM27_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNR07305.1"
                     /translation="MTREALLQFLGTITDPLTGQNLVLANRIQNIQIENRRIGIIFSF
                     PPKLESRIKSELHEAISTGLMESAPGYEVDLHFGNPGAGEKKSSNLPQIRNIIAVASG
                     KGGVGKSTISVNLALGLKNLGLKVGLLDADLYGPSIPIMLGLKGKRPQVRQVYGVSKI
                     VPLEAHGIPVMSIGFVVEPEQAVVLRGPRLAGIMKQFLEDCLWPDLDVLVIDLPPGTG
                     DIQLTLVQTVPVTGAIIVTTPQQVAVADAVKAANMFRMQGIEVPILGVVENMSWFTPA
                     ELPDHQYEIFGKGGGRQLAGHFQTQLLGQIPLFMSVRSSGDEGKPAILHHPETQTVFE
                     KLARQAYESLQIRNETMPPTEKVRMNK"
     gene            28870..29814
                     /locus_tag="PKM27_08325"
     CDS             28870..29814
                     /locus_tag="PKM27_08325"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNR07306.1"
                     /translation="MEVHFGLDDIPSLQHSVITVGSFDGVHSGHRKIIEGLIASAKAF
                     DTQSVLITFHPHPRLVLNRFDQTLRMLNTLDEKIEIFKSLGIDHVVVVPFTPEFASQS
                     PKDYIEDFLIRYFKPKAVVIGYDHQFGKGREGTREILFSLQEQYHYAVREIPAHQIDG
                     VTISSTKIRHALAEGQFDLVRKYLGYPYPVSGRVIPGDQLGRKLGFPTANLMINDTTK
                     FIPQHGVYAVRIKWKSYDKPGMLYIGKRPTTHESGRLQIEVNVFDFDGEMYGEQVWVE
                     ILKFIRHDRQFEDPDALRAQILRDQQEIREYFQQETQA"
     gene            29814..30845
                     /locus_tag="PKM27_08330"
     CDS             29814..30845
                     /locus_tag="PKM27_08330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR07307.1"
                     /translation="MSRTAIVILNFNGKKYLEQFIPSILQFSPPDVQIVVGDNKSTDD
                     SLPFLRSHFPSVSIVENDRNYGFAGGYNEVLKRVPADYYFLLNSDVQLRGPWMNLISA
                     MKEDPWIVACQPKILSYRDPEYFEHAGAAGGWMDWFGYPFCKGRVLDRFEKDLHQYDK
                     PSELFWASGAAMMVRAEWFHRLGGFDADYFAHMEEIDWCWRVKKCGGRIKSVPSVSVY
                     HIGGGTLPYQSVQKVFLNYRNNLATLYKNESAGKLMLLLPARILLDFVAVLAFVLRKN
                     AKAAAAVCKAYWQFIKWIPSLNRKRKSFKAICRGKEMNTAGIYRGSLVFDFFILGKKS
                     FDSIVHELV"
     gene            30832..31554
                     /locus_tag="PKM27_08335"
     CDS             30832..31554
                     /locus_tag="PKM27_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNR07308.1"
                     /translation="MSWYKLEILFEDEDLLAVNKPSGLLSIPDRWDQEKESLYYILGE
                     SRAGLMVVHRLDKDTSGVIVFAKNQEAHRILNDQFSKNENRKIYWAIVGQPVSEPGTI
                     DTPIAADPFVAGKMTIHRKGKAALTYFKPLENFRDFTLLEVQIETGRTHQIRVHLSSI
                     GLPLAFDPLYGSDRPITIRDIKKRNLREPEETASALMARVPLHALSITLKHPKNLQEM
                     TITAPLPKDFKALLQQLRKWGR"
     gene            complement(31560..33443)
                     /locus_tag="PKM27_08340"
     CDS             complement(31560..33443)
                     /locus_tag="PKM27_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNR07309.1"
                     /translation="MLQLKNIFLKYGDRVLLDEVSVTLSDGEKVALVGRNGAGKSTLL
                     KIIAGLQSPDKGAVDVPKGTKLAYLRQEIDFDDQTLLIHEAMKAFEELNSIEEKINRL
                     EARLHDHLDEKVMGDVLHELEDLYHRRHTIGGDTAQAETEKVLTGLGFRPQDFMQPLS
                     HFSGGWKMRVELAKMLLCGPDILMLDEPTNHLDMDAIIWLENYLHTLPSTILTISHDR
                     QFLDNVSDRTLEIEQGKLNDYAAGYSKYLVQKAEREQLLENAFINQQKLIAQKERTID
                     RFRAKASKARMAQSMIKQLDKIERIDWNPTDPGSMVVRFPPAPRSGEVSIETKAIRKN
                     YGEKQVLQSVDFVARRGERIAFVGQNGQGKTTFVKILTGTEPPTSGKVTPGYNVNIGY
                     YAQNQAETLDLRKTVLDTLEEISPPEMRPRLRAVLGAFLFSGDDVDKKVSVLSGGERA
                     RLAMACMLLRPINLLILDEPTNHLDIDSKEVLKEALMDYEGTLIVVSHDREFLQDLTD
                     KTIEFKNGGIKEYLGDIQYYLSKREIGDFRELNKANPLTRQTVEPEVPAKIPVVQARQ
                     VTKSNQKLEKEIEKLERELAEMETKMFSDGFYASAQATTVLEKYQSMKKVLEEKMEAW
                     MNA"
     gene            complement(33483..34049)
                     /locus_tag="PKM27_08345"
     CDS             complement(33483..34049)
                     /locus_tag="PKM27_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNR07310.1"
                     /translation="MAKKTSKIKSGKLILAEPFMLDPHFRRSVVFLCEHHQKNGSVGF
                     IINKPLKMKINEMVEDFPEIEAELYYGGPVGTDTLHFIHNVGDLLEDSVPVIRGVYWG
                     GNFEKLKFLIERQLIGPDNIRFFVGYSGWVKGQLSEEMEVGSWIVAEGDPNYVFKIKS
                     NILWKRVLNDKGHTFSVISELPENMSWN"
     gene            complement(34067..34939)
                     /gene="fabD"
                     /locus_tag="PKM27_08350"
     CDS             complement(34067..34939)
                     /gene="fabD"
                     /locus_tag="PKM27_08350"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNR07311.1"
                     /translation="MKAYLFPGQGAQFAGMASKLAGYPEANEIFGTANEILGFDIFEV
                     MRNGTEEDLRQTRITQPAIFLHSVISATVAADFRPEAVAGHSLGEFSALVAAGALRFE
                     DGLRLVYTRALAMQKACDLTPGTMAAIVGMEDAVVEKVCAETKGVVVAANYNCPGQLV
                     ISGEIDAVQQAMQNLRELGCRNAVQLAVGGAFHSPLMKLAEDELMAGIETTVFSSPAC
                     PVYQNVDAAPHRDPDEIRHNLVRQLTAPVKWSQTIQQMISDGFSEFVEVGGNGKVLSG
                     LLKRIDRKVPVSSI"
     gene            complement(34936..35574)
                     /gene="folE"
                     /locus_tag="PKM27_08355"
     CDS             complement(34936..35574)
                     /gene="folE"
                     /locus_tag="PKM27_08355"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNR07312.1"
                     /translation="MYLCTRKYRPSGMAYKKNETYDRAVTETLTGHYQEVLRALGEDP
                     AREGLIKTPERVAKALQYLTKGYSENGAAILRSAMFEEKYSEMVLVKDIELYSLCEHH
                     MLPFIGKAHIAYIPNGRIVGLSKLPRIVDVFARRLQVQERLTDEILHCINDTLEPLGV
                     AIVIEAMHLCMMMRGVQKQNSITTTSAFTGEFRNAETRSEFLSLINFKTTKT"
     gene            35672..36709
                     /locus_tag="PKM27_08360"
     CDS             35672..36709
                     /locus_tag="PKM27_08360"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNR07313.1"
                     /translation="MPTLSLPKVSISRLWLHGTIWMIFLVLLVLMDNNPYSLGLSFLN
                     NGSRLMLYGLLIYFNFNYLIPRYLNTNKLAIYFGSLVVLALIATPLGTLVQYFLLSSY
                     PQLQGNIQSQQGPLFLVSMMFLIFSTSGKIIVDWFKQQQTMRDLETQNMQSELKFLKT
                     QINPHFLFNTLNSLYALTLKKSEQAPEIVVKLSEMMRYMLYECNERRVPLEKEVKYIQ
                     NYLDLEALRQSNLVDIDFKVKGDISKHKIAPLVFIPFLENSFKHGVNHQLSDAYVYAT
                     LAVEGDELDFKIENSRSTGLQNLGPKPQGGIGLINVRRRLELIYPGHHSIDIEDQPHT
                     YTVHLKLKLTP"
     gene            36715..37434
                     /locus_tag="PKM27_08365"
     CDS             36715..37434
                     /locus_tag="PKM27_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR07314.1"
                     /translation="MLKTIIVDDEPLALDVLEEFIKKVPELKLVKKCQNALEAGEAIR
                     KNEVDLIFLDIQMPQITGIEFIRSLPKPPKVIFTTAYSQYALEGFELNAVDYLLKPIS
                     FDRFLKAVNKALDTGYKQETETEVPEDGPDFIFVKADKKLIRLKYSEIQYVEGLKDYV
                     IIKTDTGRIITLQTMKSLEEKLPQPTFMRIHRSYIVNLEKINAVVGNMVEVTEKGQPK
                     LLPVGKNYREELEKVIDKNRL"
     gene            complement(37503..37982)
                     /locus_tag="PKM27_08370"
     CDS             complement(37503..37982)
                     /locus_tag="PKM27_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR07315.1"
                     /translation="MSRFSTKRADTAPTISTSSLPDIIFILLFFFMVVVKLRESNLKV
                     KAVTPYATELTKLEKKSLVNTMYIGRPLSQFVNQYGTKPRLQLNDKIADLNEIPLFLE
                     RHKVKLPEGQRPGITTSMKIDGDVTMGFVSDVKTELRKAGQLKVNYSAKKRTERSGF"
     gene            complement(38011..38565)
                     /locus_tag="PKM27_08375"
     CDS             complement(38011..38565)
                     /locus_tag="PKM27_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR07316.1"
                     /translation="MLAKKKKKANPEINASSMADIAFLLLIFFLVTTNIVEDKGVLVR
                     LPQWSEEPPIDLTLNTRNVFTVLVNAQDQLLVRGEPVKIEDLKTRAKEFISNPGRRED
                     LAESAKEAIISLKNDRGTHFKQYLAVYNELKAAYSELWNEEAKKRFRKDTIEECNKEQ
                     QNEIKRDIPFVLSEAEPTAFGEEK"
     gene            complement(38580..39059)
                     /locus_tag="PKM27_08380"
     CDS             complement(38580..39059)
                     /locus_tag="PKM27_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07317.1"
                     /translation="MVNLYKLLSGKGTFFAFALALVAMIIFAVPVLNGLDSFNALPTE
                     QQKTSNIFNMGIVIMLLMLVVAVVITVLWSVIHVAMNPAGAKMGLIWLAVIVGLFALG
                     YFVLNAPDNQAILDDLKTNEVSANMSKLLGGGIWLMLLMLLGAVAIFIFSEIRNLFK"
     gene            complement(39108..>39501)
                     /locus_tag="PKM27_08385"
     CDS             complement(39108..>39501)
                     /locus_tag="PKM27_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR07318.1"
                     /translation="LEGLRHAEKGPDAVEKAISAFGGMQMSLLERGLVWISLFIALAP
                     MLGFLGTVVGMVQAFDRIEAAGDISPTIVAGGIKVALLTTIFGLISAIILQIFYNYIV
                     VKIDTITHKMEESSIGLVDILTKNKIFK"
BASE COUNT         9264 a        10009 c        10051 g        10177 t
ORIGIN      
        1 ttcaatgatg atcgcgggat catcggactg catcatggtt tgataaaatc cggcggcctg
       61 gaccatgttg cggggaacca ggatatgtat tccccggagt gaattcaaca tcatgcccat
      121 gggcgaaccg gaatgccaga tgccttccag gcggtggcca cgggtacgga tgatgagcgg
      181 ggcacattgg acgccattgc tccggtagcg aaggcaagcc aggtcatcgg acagggggga
      241 caaagcatag atcaggtaat ccaggtattg gatctcagcg atcggacgga gacccctcat
      301 ggccatccca atcgcctgac ccactatggt ccactcccgg atgccggtat caaagatccg
      361 ttcttcccca tacttcattt gcaggcctgc aaagccctgg ttgacatccc cgatctttcc
      421 cacatcctct ccgaaggcta tgactcttgg attcgattca aataatttat cgaaatacag
      481 gtttaataat ttatatccgc tcagggtggg tgcatggtcc gaataaaccg gaggtatggg
      541 ttttacccgg atggcggcat tgcgggtttc actggtctgg tgggtatgaa accgcaagtg
      601 ggctttttga tatacctcgt tgagccaatt cttgagttta gtccatacgg gatccttctc
      661 ctgatcggga gtggcgatca acaactgtcg aatggaccgg gcgaggtcac tgcttaccgg
      721 ttcgcggagg gttctcagtt catcgatatg atgctggacc cgtcccgcat gatatggcct
      781 cgaggcagca acttcagcaa gcaggtccac gaccaactcc aattcctttc gggtgtggtc
      841 aaaggcatgg gccagtgcgc ggtccctttc tttttttaca aactctttgg ccttgatttt
      901 aagggcctca tcctcctgga ggcttaacag ccctgcatca agcaaccagc tccgcatcac
      961 ggaaattcca tcgaattttt tttcccagtc cagacggtct ttggacttat atctttcatg
     1021 cgacccggaa gtcgaatggc cctggggctg ggtcatttcc tggacatgga ccaggaccgg
     1081 agtggaatag aggcgggaga tctgggtagc tttttcaaaa accttgcaga gttcaggata
     1141 atcccagcct tttacggtga aaatctggat gccttcgccc tggctttcac cgaggaagcc
     1201 ttccagggcc ttgctgatgg aggctttcac ggtctgcaga tggaccggta ccgaaatccc
     1261 gtatccatca tcccagacaa tcatgacgag cggcacctgc ataatggcgg cggcattcat
     1321 ggtctcccaa aaaggtcctt ctgcggtgcc tccatccccg atcgtgcaga aaacgatttc
     1381 agtacccttg ttggagaatg gcgtgccgtc aaggtctgcc cggcttttat agatgcaaga
     1441 ggcctgtgcg agtcccaggg cccggcctac ctgacccgcg acgcaggaaa catccgaaat
     1501 gacattgtac ttatcgcggt gtgccgtcca gttgccgtcc tcgccgatca atggagttgc
     1561 aaaatgattg ttcatctgcc ttcccccgga aaagggatcg ttatccgaat cggaatacat
     1621 ctgaccgaaa aattcataga ccgaacaaag gcccagggaa aacatgatgg tctgatcgcg
     1681 gtaatatcct gaacgaaaat caccgggctg aatataatgt gacaaggcaa cctggggtat
     1741 ttccttaccg tccccgaaaa tgccaaactt ccctttaccg ctcaatacat ccttgcggcc
     1801 cagaatactg acttcacggc tcaccagcga aatccagtaa tcctttaaaa tccttgcctc
     1861 catggtgtcg cctgcaatac ccgtttccgg accggcttcc agcagaagtg catccctgtt
     1921 catcagcttg gttttaaaat caggggcaag atacgatttt ggaacggata ccttccttac
     1981 ctccggcggg aaaaggcttc cggaatggac tggattccag gcagtatagg ccatacccta
     2041 atcccataag tttaacagag ggttaacata aaagacgcaa gcggtttgtt attctatttt
     2101 caaatgatta aatttgtatc cattaaacac atttccatga aaaaattatt gtctctttct
     2161 accctgtgcc tgctggccgg cattgctttt gcccaaaaca atgccccggc cgaagtcaaa
     2221 accggccctg tgatgaccct gagcagtgat gttgtcgatt atggcgaaat catgcagaat
     2281 gccgatccgg tacgcaaagt aaaatttacc aatacaggca ctgaacccct gatcatcaaa
     2341 aatgcgcagg gcagttgcgg ttgtaccgtt ccgctctggc ctaaagaacc catcatgccc
     2401 ggagaaagca gtgagattga aattcattat gccacagacc gcatcggcca gattatgaaa
     2461 acggtgacca tcaccaccaa tgaaaccgga gtcaaccatg tcctccaggt aaaaggaaat
     2521 gtaaaaccca aaccggtcga tgaaaccgtc cccaccaaac aggctaccct gcttacccct
     2581 gttaagaaac aaaacagcaa ccagtgaatt attcattcct gaaaaaagcc tgaatggggt
     2641 tccattcagg ctttttttat tggattagcc tgctctcccc ttccaaaaag cttctggcat
     2701 tggtaattgg aatcagccca atccaagagg ttgctccagg gctgccattc cgaaagcttg
     2761 gaatcccggc actcgcctgg agctttttag ccggatgttc atggaggatt ttcaatataa
     2821 tatttttcaa tggtgcgaac tagtttcagg cgaggacgcc tgaaacaacc tccttatcgc
     2881 taaaacattt ggctacactt taatggactt ttatctttga cttttcagac accccttttt
     2941 ttattggctt atagtctagg ttaatccttt attatgagac gccagctttt gatcagcccg
     3001 ggcgggtgaa gaaaatgggt ggaatcagat tcttccgggc atacgaactc aacccttgaa
     3061 ccaggctaac ttgctaactt gctatctaat ccaggtacaa cttaaattcc accctcctgt
     3121 tcgtagccct gccctgttgg gtttcattgg tggcgatggg cctggactgt ccaaagcccc
     3181 tggaggagac cctggcccag gccagtcctt tgctgatgag gtaatcacgg caggccctgg
     3241 ctcgttgttc acttaatctt ttattaaaat ccgcactgcc ggtattgtcg gtatgtccgg
     3301 agatctcgat tttgtactct ggataattcc tgaggatctg cgagatctgg tcgaggatcc
     3361 ggtaggaatc gttggtcagg gtggcgctgt tgtgctggaa ctgtacgtcc cgcatggcta
     3421 tattcaatac ttccttgtcc tcctgtttta tttccgggca accccggttg gaagcgggac
     3481 caaaggtatt ggggcaacgg tcttccccat catcgacgcc gtctccgtcc gtatccgggc
     3541 agccgttttt ggacttaccg gctacattgg ggcatttatc tacccggtcc gcaaccccat
     3601 ccccatcctt gtcggggcaa ccgcgggcgg agggtgagcc ggcaaccgta gggcattcgt
     3661 cgagatcatc gatcacccca tcattgtcgg tatccgtgga ggggcaaccc ctgtttagtt
     3721 ttgtaccggc caggttcgga cagtcgtcat ccttgtccgg gaccccgtcc ccatcgctgt
     3781 ccgggcagcc tctcaatgct ttgactccgg gtatttcagg gcaaagatcg ttgggatctt
     3841 caatgccgtc gccatcggta tcgggacatc cggcaaactg tttaagtccc ggtttatttg
     3901 gacacaggtc caggtcatcc ggtatgccat cgccatcgga atccttggcc tcggacacgg
     3961 ctttttcttc ttttttggcg gctcccttcc cgaaataatg aatgaagcca acagagaact
     4021 ggttgttgtt gcgttggtcg gaaagggaca agcggaattc agcctggaaa ttgatatagg
     4081 tagacctcgc aatttttaca tcgaggccgg cccccatagg gagttgtacg ttaaaggtat
     4141 ccaggttttc cgtattgacc ccggcgccga gcagaagata aggccggagg gtgccttttt
     4201 tagaattgaa gcggtactgg agctgggcat ccagttctcc gaaagtgatg ttttccgact
     4261 ggtcattgac gctgaacaca ccgattcttg cgggaatata cagacggagg ttatccgtga
     4321 agttcctcaa ataaccgact tcaaacccgc tgttgaattc gcggaaagac ccgaaattgg
     4381 acagggagcc ggcattgggg cttttataat caatgaaagc tcttttgaaa ctgatcccgt
     4441 taacagaact tcctgcctgc gcgggtgggg tttgcgaaag ggtggacagg gtatcctggc
     4501 caaacaatgt tgaagtgcct gtcagcagga tgaacagata tacaacattt ttcataagac
     4561 gatttttaaa ttgttgatag ccaatcatta aacggatgta cacggtaaac ccgtcaaatg
     4621 tacatcgaat tttttatatg tggaatattc ctaatgcaaa aaatattggt attcaatact
     4681 attttaaagg aaagggaagc gggatctcaa ccagcccagg atctatgctt cttcaaagaa
     4741 gccggtatcc atatcctttc tcaccttgtc ccaatcaaaa taccggccgt tcatggcgag
     4801 gtataccccg gggctcaggg tttgcacaaa ggcaagggcg gctccgagat tgaagaatcc
     4861 gtcggatgaa tttccaaagg cataagggat cattgcccca gtcaggataa tggtcttccc
     4921 ttcgatcccc gcgcgggcga tttcagtcgc cgtggccacc atccggtcgg tgccgtgggt
     4981 gaggatgatc ctgcggtgcg ggcttctctt gcagttgtga atgatgatgc tccggtcggc
     5041 atcggtaagg tccagactgt ctaccatcat cagggtttta atatctatgt ccagtttgca
     5101 tcttcccctt gcaaacattt ctttcagatg ggtatccttg aaatacaatt caccggtgat
     5161 caggttgtat tccttgtcaa aggttccgcc ggtcacaaac acctggatgg tttccatgcc
     5221 tcaaagataa acaccccatc cggacggccg gaaggagcct ctgtcttatc tttgggaaat
     5281 gctttattta cacaggtcat tctggttgat gttttttatg atgcgggcgg aatttggctt
     5341 gtttgcccag gatcctgaag gactgaatcc tatgcagggg gtggtggacg tccagcaata
     5401 tgacctggaa ttatggatcc aaccgggtga taccaccatc cgcggaaaaa ccaccattga
     5461 tctgcaaata gtgaaaacca cacgcgagct gatccttcag ttcgaggatc tccgcatcaa
     5521 tgcgctcacc tcaaattcgg ggaaaatcaa gtatacctac gacgggaaac agatcatgat
     5581 ccggacgaaa aagaaattaa aaccgggcag atccatccgt ctggtcattg aatatgaagg
     5641 caaaccctct gatggcctga tcatcaggca aaacgaggga aggtacaccg tctttgccga
     5701 taactgggcc agccgtgccc gaaactggtt tccctccgtg gaccaccctt ccgacaaagc
     5761 gcgttttcac gctacggtgc atacggccga cccttatacc gtcatcgcca acgggattaa
     5821 ggtcggggaa tccggcctga atggtaaaaa atcttttgta taccatatgg atcatcccat
     5881 tcccacctat tgtatggtga tcggcctggc gaagatgagc atagccggcg accggacaaa
     5941 gggtggaatc cccctctttt attataccta cccggaagac agcgcccctg cacgtgaaag
     6001 cttcaaacgg gttgctgata tggtccattt ttacgacagc ctcataggaa aatatcctta
     6061 tgaccggctc tccctggttc aatcgtccac ccgttttgga ggaatggaaa acagcagcgc
     6121 gatttttctt gcggaccagg gcgcggttta ccggagaagg tctgatccgg agggtttact
     6181 ggcacatgag atcgcccacc aatggtttgg ggacgcagtt acccaatctt cctgggccga
     6241 cctctggctg agcgaaggat tcgccactta tttttcagcc ttgtattttg aggcgcgcga
     6301 tggcaaaaac aagttcgact cgatcatgga ccggtcccgg aatgaatacc ttcgacgctc
     6361 accccggaaa gccccggtga tctatgatgg atacccccag cttttccagt tgatgaatgc
     6421 ggaaaattat aataaaggag gtttattcct tcatgcgtta aggcaggctg cgggggacag
     6481 cgcttttttt tccgctatcc gaaaatatta cgcacagttt aaacaccgga atgccaccac
     6541 ggctgatttt caggctttgg cagaggccga aagccgtaag gacctggatg agctgttcac
     6601 cctttggttg cgccgtcccg ggttacccga ataaggaaga aaaaaaacgg gaatccgttt
     6661 tcacagatcc ccgctcggtt attttgatgg gtttggatcg ttcagaccgg cgcctcgttt
     6721 ttgacgagga gctggaatcc attgccatgg atgtttacga tttcaaggtc cgggtcgtcc
     6781 ttgaggtatt tgcgcagttt tgtaacaaag acatccatac tgcgtgcagt gaaataattg
     6841 tcttcccccc agattttagt cagggcctct gaccgggcga gcacgtcgtt tttgtacatg
     6901 caaaacatgc ggagcaaatg cgcttctttt ggagaaagtt tgtccttttt attggtcacg
     6961 gtattgacca gcaggcgcag cggataatta aaatgatatt ttccgatcgt aaattctttg
     7021 atctcctctc tgggatcggc cgcttttttg ctgcgtttca ggatagcctg gatccggtac
     7081 aacaattctt cggaattgaa gggtttggag atgtagtcat ccgccccggt tttgaaccct
     7141 tccaggatgt cctgcttcag ggtttttgca gtaaggaaga caataggcat ttccggatct
     7201 ttctgcctga cttctttggc aagggtgaat ccgtcttttt taggcatcat cacgtcgaag
     7261 atgcaaagat caaaatggtc cttcctgaat gcttcataac cttcctggcc atccgtagcg
     7321 agcgttactt caaaatcgtg catctcaaga taagaccgga gcacatcacc aaagttttga
     7381 tcatcttcaa ccaacaggat cttattgttc gtttttacca tataggatta gaattttgta
     7441 tacaatgtta acgttaaaac cactgattta attttaacaa aaagtgaaat tttaacaatt
     7501 tttgcccgaa ataatttatt tcataaaaaa ttattattta atagattgta aatctgaaga
     7561 ttagaataaa atggacgaca aaagtcccgg aggtcaactc cctaaatacc gggaggctac
     7621 aaatttttgt tctgatgttt tggaaaatac aagataaaat tactgccttt gcctaaggca
     7681 cttttaacct gtatatgacc ctggtgggca tccaccatga cttttacata gcttagtccc
     7741 aggccgaaac cttttacatc gtgcaggttc ccggtgctga cccggtaaaa tttttcgaaa
     7801 atgtactttt gattttcacg gcttatgcct atccccttat cctcgatcga tatctcgata
     7861 ccgtggtgga tgttcctcgt cgcgacccgg atatccggcg agtccgggga atatttgttg
     7921 gcattgtcca ggagattgta aaggatattg ctgatatggg tcagatcccc ttcaatcacc
     7981 gggtctttgg cttccaggtt cattttcaga tcgccttttt ttcctttgat ctgcaggttg
     8041 acgttttctg aaatcgcttg caggagttca tggatattga tttcggaaag tttaagttgc
     8101 aagtctcttt tttcaagctg ggccagttgc aaaacttttt cgacctgacc gagcatgcgg
     8161 gcgttttcct gcttgatgat ccgtgtgaac cgctgcactt tatcctggtt ggaaatgatg
     8221 gaaggattgt tgatggagtc ggtggccagg gaaatggttg ctatcggcgt cttgaattcg
     8281 tgggtcatgt tgttgatgaa atcatttttc atgtccgata tttttttctg gcgctggatg
     8341 atatatatgg tataggcgaa acagaccagg atcagggaag taaaaaggag ggaccccacc
     8401 gccgcgggga tcaggttttt ccaaatgatc tggttgcgat gcggaaggta gaggaacagc
     8461 ctgccggaac cctggctgct ccggtcaaaa atgttgattt tataaggggt ataatagagg
     8521 cccgatttga tgcccgtccg ggaaagggac gatttaaggg aggtgaccgt aaaaaaatta
     8581 tcaaccacca caaaacctga atcgcggttg gaataaatgc cgtagtggta ttccgggcgg
     8641 ttgcccagct tatgcacggc ccttttcagg agggaatcga taaacagaaa gccggcgcgt
     8701 ccgtccagcc tctcgatgat gcccttgttc atgtattcgg tgatcagttt ctggctttcg
     8761 gccggaggca ggtcggcgag gggctccagg ttccggttgt ccctccgttc cacttcatca
     8821 atcacgccga ccagagcctg gttgacctcg cggtcaaact ggttttcctg ctccctcaaa
     8881 gtgagcctga tccaatacat ctggagcagg atagacccca ggagggcgag gcccatggat
     8941 ataatgatca accaaatggt tcttttggtc atgtcagccc tgtctggtcc tttttatgaa
     9001 taatcggtcc ggtcatgatc cgcatgcagg taagcaaacg gcttgaaagc gtcagcaaaa
     9061 atacggttaa ttatttcagg gcaagccgga cggtgactcc gcctttcgct gtggtgatgg
     9121 gaaaggattc ctgcaccgcc gggataatcc ggctgtaatc aaaaaagagc cgaagggtca
     9181 cccggtcgtt gacttcgtaa tcggctgcgt tggcaatccg gagcattttg gctccccggg
     9241 tcggcacgtt ttggtcctgg tcgagcaccc ggttattggt caggtcatcc cggaaactga
     9301 aatcaaattt caggttaagg tcacttcctt tttttgcctg gttggaggtt ctgccgcggg
     9361 catcgggagt ttggttgtcc gtgggggcat taggcctggt gcgctggggc gcttttttgc
     9421 cgaagggcat gactacgttt ttaaccctcc agccgaaacc cacggtgatc tccttggaat
     9481 tttgttcgat cagctggtaa tcgatcatat tgagttgcag gctgcgctgg atcgtatagt
     9541 caaaattcaa ttgcaggtcc gagagggtct tgatatcaat cccgatcagc ggattgaagg
     9601 tttcggtcat gaccacgttg gggatttcaa tcctggaata atagtctgca gtcagcggct
     9661 tggtgcggta ggcggaatcc ggatcataaa acacatccga gttaaactgg ttgaccatga
     9721 gccgggaagt atatccatgg cggatgcttg ccccggacaa gaagttcttc aggaagggga
     9781 gtttatggag gccgttatag gtcagttgcc agttgggcct gggattggtt ttgaatacgt
     9841 ccaggccgga agactgcggg tccaggccgg tataggtggc gatgaaagct gggatgatga
     9901 cctgctggtt tttcctgccg aagccttcct tatatccttc attcaggggg tggtcgccgg
     9961 catccggttt gttgggctgg cgctccgaaa atatggccct gttcctttca aattgcgcat
    10021 acaggctatc cacatttttt ccgaagagcg tgttgatacc caggtaagaa atattgaagg
    10081 aacccacttc agtgggcgtg agatgtttga agcctccgcc caatccgtca tttttaaaga
    10141 attcaagata gttgcgggtg tagtttttgt tgagcgaaaa ttcgatgctg aaatcattga
    10201 agggttcaag ggtcaggcgg gcatccaggg tttggttgta attcctggag aattgctggt
    10261 tcagccggat atcgtccgtg atcattcctt tttgggcaat atcttcaagc caggtctggc
    10321 tcgggtccat gcccaggata taagcccagc ccggttcccc aaatccattg gccagcccaa
    10381 ggtattttgg cagggaccgg aaccccggta ccagggaagc caggttttca ttgtaggtga
    10441 gccggcctcg cctcaaactc attaagggcc ggatcaaggc tctttcaatc catgaaggtt
    10501 gtttttccgc cctttccctg gtgttacggg caggctggcc ggattggtca gcgggtgcgt
    10561 tcctgtcccg ggtgcgcgcc ggttggccct ggccggaagg gggttgattg atcttttgca
    10621 gataggccca tttatcgtac agggcatcaa aattcaggtt tgcggacaat tgcctggtct
    10681 ggctgttctg aatgacattg cccaaaaaat ccaccacgtt ctctgatccc gccagccagg
    10741 caaaggtgcc cgtgccctgg gcggtcacct gtacccaatc cagcaaaggg atgtgccggg
    10801 taggcaggtt ataactcgca ttgtaattgt gcatgtagtt tttgggcctt cccaggctgc
    10861 ggatgttatc ccagatgact tctttatttc ttttagggtc ataggtaggg tccagccggc
    10921 ccaggctatc catcacggcg agctcatcga tcgctgaatt gttggatgca ttgaaggaaa
    10981 atttgaagga ctgggtaagg tcccactgaa gattgtattt ccggtcccag aaaaacaatt
    11041 tcgtgatagc cctgccgtat tcaggggtcg aaaaccggaa agcccgagtc ccgtatctac
    11101 ggtccagccc cgtcgaaaag gaaaagctgt tgggcagggg attgatgtta aactgggtga
    11161 accaccggac cagtttgtcg tttttgacca gtttgcggaa gggggtcagg tatttcgcct
    11221 gccgggcaaa attgtagttg aatcctccct tatatacatt ttcctggtct ttttcgacga
    11281 tgggatcgtg gttggtggtt tgggtaaagg aatagttgaa gttgaaattg gagatgtccc
    11341 agggcgcagg tttgctgtcc ccggtccttt ccttgcgtac attggtaaaa ttgaagctgc
    11401 ggatggattc gtaagcctgg gcctggcggc gaatggagtc cttctcactg ctgctgcgtg
    11461 tttcgagcaa tttgtccttc agttttatat ccaggtcgta gggatcaaat ttcggagcgc
    11521 tggcattcac actgagctgg gtgtaaaacg ggattttgat gccccatttt gaaggcaaaa
    11581 atttacccag ttccaggttg gaattgatgt caaattgttt gaggtcttcc tgtcgccgct
    11641 cgagcacctt ctgatccagg gccccccacc cgatgctgtt catattgccg ctgatggaaa
    11701 ggtcacccag gtcggccagc tggatgtcca gtcttccgat ggcgccgtat cctccctgtt
    11761 cgttgaggcc gttcaaccgc aattcgttga accaaacatc cgcttcatgc tgcctggcgc
    11821 tgcggaccgg aaaacgcatg ccgagcatga cctgctttac ctttccgagc gtggggttcc
    11881 caactatggc aatgatcctt tccgggtttt ccggatcggg gatggcgtat tcaaggtcca
    11941 gcggtgcgtt gtttgcattt ctttcttttt tcaattccac cagggcctgc aggctcaggt
    12001 ccagttcgtt ttccaccttc cagatttctg tggattccct tcccgggatc acctggtccc
    12061 ttttactgag ccgggtggga atgcggtact cgtaataatt gctggtaaaa tcgcttccaa
    12121 tccggagaaa ggtggtgaac tcctccgtgc cgaggtcctc cggagaatcg gcatggacaa
    12181 acatgcggat ccgctcatag ttgcgcatgt ccatgcggat gggtttatac acggcgcacc
    12241 cgcagcctcc tttcagttct ttcacggaaa gggaaatgga ttgttcgttt tgaagcagat
    12301 tgggaaaggg tccaatcgag ttttcacgct ggatcccggc aggcactacg tagggaatcg
    12361 gaaaacgctg gctgttttct tcaatattga catcgttgac atcgaattta aaactgttgt
    12421 cgatgtcatc gctcgcgcag agcggcagcc ggtacctcct ccactggttc ctgaccatgt
    12481 cgatggaatt catcctgagg gttacggact gatcaaaatc ggccatatac atccggatga
    12541 agcggatgtt gcgcaatccg ctgatgctgc cgaccgtggt atccgggctt tccaaaggaa
    12601 tttttaccct gtaccagatt cttccgccgg ggccggaaat ggtgtcggtg atgtatttat
    12661 tgatcgccat gccccctgca ttatccgggt acatgggcac gcggtactca taataagctt
    12721 cactctggtc gaaggcattg tcgtcattga tgtcctccga ttccgggatc tgagtgtaat
    12781 ttttggcttc cgttcccgac tgttcggtaa aactgctgtt tccctggggg ttgttccagt
    12841 ccttataccg ggtatagatg ttatcggtat tggtgaacga ctcgtcatta aaatatttat
    12901 agttgtcgtt ggccggatct tcccggatcc gttcatagac cagcgggttc agggactgta
    12961 ccttttgaag gtaatctgca taatatcgcc tttctccgtc atcatccagt ccgtcaaagc
    13021 caaggtcctg ttgtttcagg atttccgggt cgttgttgtg cgcacgcgtg atggcctgca
    13081 ccagcgggat ccggccccag gccgtcgaat cgatgcgggt catgtttttg gtggtcggca
    13141 atccgttttc aaagtacatg agcccgtctt tcatgatgtc ttcggaaata tccccgaggt
    13201 ttatatagag gtttcccccg gtagtcaccg gagaaccatc ggatttggac ataaaaggat
    13261 tgaggaccca gaattcgatg gtttcaatat tggccgcttc aaaatcggta ttatacagtc
    13321 cccgttgaat accgccccag cgtgacctgg gctccagcag gttcccgttt tgatccagtc
    13381 ctgcggaata aggggtgcct cccggaacat caaaattgta aggtccgcgc tggtgcggat
    13441 taaaattcag gttgaaggta tacaattcga tattctgacc tggtccgatg tttcggtttg
    13501 gaaacaattc ggtaatgttg atggcggtgg aataggcgtc ttcgttgccc ccccttaccc
    13561 tgcggtcgat ccggtaccag ctcattttag cccggttgac gagcggaaat gtggtgttcg
    13621 aattttgggg agcggattcg cggaacctcc ttccacttgg cgtgctggcc ggaaaccatt
    13681 gattgaccgg aatgcctagg tcctggctgc tggtgctccc ttcaaagtca tcaatatagg
    13741 cggtgcctcc tttgtccttg ccctggttga tcgccctgga gtgtccgggc cttaaagcgg
    13801 ctgcttcgcc caggaaggat attttggaat tttcttttgt ggataataaa ggcaaccggt
    13861 ccagcaagcg ggtgatccag ggcgcctcgt tgttgtacac caggtccagc ccgtagattc
    13921 cgttgttgat ggggtcctcc ccgatattta ctttttgcgt ataaggcctt tcaaacaaac
    13981 gcatcacggt cccccccacc gaaaggtgtt tgttgaattc atattcgccg cggagcccga
    14041 acatcgtccg gttgttattg aacccaaaaa gggaattgtc ttcataatct atggaaaccg
    14101 gaaccccgga actcagaatg gccgggtcgg tgaccctcac cctgccggtc tggtaatcga
    14161 tctcatattg gttgggggac aattcctggg ccccggccct cacccgtacg gatccctggg
    14221 gtatattaaa ggaattgaga gccacatccc ccccgctcga tgatttatac tccccccgga
    14281 tgatgtaccg gttggcttcc ggaaaattta gggaagcggc cttggtggaa tcgtagagtt
    14341 gctggaaaac atactttttt ctcaggtctg gatcggagat ctttttcccc aggctttccc
    14401 cgaagggctc cagcaccggg aacatcattc ttccgctttg aggatcgatg gtcagtccgg
    14461 gcacaaaatc aaagactccg tcgggaaagg ggtcccgttg tttgttgagt ttgtcgaggt
    14521 tgagcaggtt gaggaggggg atgcttttca gttccgtctc cgggagaaaa cgtttttccc
    14581 ctgccccggg gtcgtcgaat aaaacatcca gtttaaaatc ctcttcactg atgccaaagg
    14641 accgcaggga atagaaattt ttcatcatca ggtcccagat ggggatatcc acccggttgg
    14701 agcggggctt gaccagtttg gtgacgatga cgttgagcga atccggggca acttcctggg
    14761 taaattcccc cactttataa gcttttccgt tgaaggtgta tttataggaa atggccacga
    14821 tgtcgctggg ccgcacgaca cggcggaaag agataaatcc gagatcggga tgaaccgtat
    14881 attcccgctg gtccagcaac tttcctttga acacttcaaa gtccctgccg atggcaaagt
    14941 taaaaggggg ccgttgcagc ccgctgacca ccttgtccgt ttgccgaagg cttggatcat
    15001 tgataaactg ctggtaaatg cggttggtcc cgttggtggg cagtttctgg tttgtggtca
    15061 tgtcgatgac cggggtcgat cccatcctcc agaagtccgg gtcgggattg ttcatccggt
    15121 cgtattcccc caggtcggaa aaagccacca cctgtctcag gttgtttata tccctggggt
    15181 ccgggctgac ccagacctgg atgctgttga ggtcgatctt cgccagggta ctgatatagg
    15241 gcaggcgaac catggccccc tcaaaagtat tcctgaaata atgtcccagt aaataatgtt
    15301 gattctcatc gtattcatcg cccggaattt caaaatcctg aagcagggcc ccgccctgca
    15361 ggttgatccg ctgttgctgg gtccttgact gggaggcgat cccggtaagc cggaggtggc
    15421 caaactgtgt ttctgccttt accccgaaga gtttttgcgc cccctggatc aggctggtcc
    15481 tcaggggcag gctgacgtct ccggcatcaa tttttttaat gatttcatct tcactgaact
    15541 gatcgctgtt gtaattcagt ttcatcacgt tgtcaaaatc aaaggtggcc tggctgttgt
    15601 agctggtatt gaggttgagt ttctcaccga tcttgccctg gaggttcatc tggatgttca
    15661 tgtcaaagtc aaaattggtc tgcctctgct gacgaatggt cagcgacggg tttttgacat
    15721 tttgataatt tgccccgaag gtgagatcga cgttaccctg cggcctgatt tcgacggtat
    15781 tgcctccaaa caggcgatcg atcagggtct tcttgacatc gattttctcc accggatccc
    15841 ttcccgtggt ctggcgccct gaggaactgc ccgcgagctc gttgaaataa tcctgctgga
    15901 gctgtttgtt tttatatcgc acatattcct caaaagtcat cgattgggtg ctgaggtatt
    15961 cggagccgat cctttcggtc aggatgtacc ggttggtctc cggatcgtat tgcacttcct
    16021 tttctatcga ggccgggtcc cggaggtcga tgggatcttt tttatcctgg ggaagccgga
    16081 ttttttgggc gggagtggta tcaggaatat gcgttaaact ttccttaatg tttccgggat
    16141 tgggcggaag ggtagccttc gatgaatttg cgaaacctga gacaatccaa atactacaaa
    16201 aaccaagtag aaatgacttc attttaatgg ttaagtgata tttccccgcg ggcatatcga
    16261 atttacaatg atcagaggtt gatgcgtcca caaatatcag cggcgaaatt aataataact
    16321 ttctaattta aaaatagtta tgcattaatt ccagcattaa tatgaccccc ttcatactca
    16381 ggatgacatt tgtttcaagg ccagcttgat caactcttcc acgctttgga tggcttgctt
    16441 ctgaaggatc ccggcaatca cctgttggct tttctgcctt tggaacccca atgcaatcaa
    16501 tgcagataac gattctgcct gcaaagtatt gtgatagacc ggaccggcgg ggactgattt
    16561 ggattcgggc tccctgccta tcttgtcttt gagatccaag aggatacgtt tggctgtttt
    16621 ttcacctatg cctttgatgg ctttgaaagt gaggtcgtcc tcgcgcagga tggccgattt
    16681 gatggcatcc ggggtaaggg cggacaaaat caactgggcc gttcccgttc cgaccccgtt
    16741 tacggaaatc agttgaagga aaagctgttt ttcatccaac tgtgcaaatc cgtagaggac
    16801 atgggcgtct tcctttacgg cgagatgcgt atacaacaac acctcttctt ttttatcgat
    16861 ctggctgtag gtatgcaggc tgatgtgcac gtgataccct accccatggc agtccacgac
    16921 ggccagggtg ggatggatca cagacagttt ccctttgaga taataataca tataatattt
    16981 ttatatataa atttagccgg cgcaaatttt gcaaatatta ttgagacttg ggaagtaaaa
    17041 ttccccggat ggcccggatt ccgggtgagg aagtaaggga gctgaaaatc gctccatccc
    17101 gaatcctttg ttccccagaa ctttcaaatc actacattta gcccacaatt cttagtcctg
    17161 aatgcgtcat agcttttttt gcattgatgc ccatacctgc ggcaatccgg tgagactggt
    17221 tgcaggcggc gcgcccatgc tcagcggaaa caacatgagc gaaaagaggc agcatttcct
    17281 ccgtgagtat gactggatca ggcagggcct gatgtttgaa ccccggggac atgatatgat
    17341 gagcggaagc atcctttatc ctccccacga cccggccaat gattttgcac tcctgttcat
    17401 cgaaacctcc ggatgcctgc ccatgtgcgg ccacgggacg atcgggacca ttaccattgc
    17461 catcgagcat ggcctcatcc ggccgaagac ccccggaaaa ctcagtttgg aagcccccgc
    17521 cggcctggtc ctgatcgaat atcgccagga aggcgataag gtagaatccg tgaaactgac
    17581 caatgtgccc tctttcctgg aggctgccgg ggtggaagtg gaatgtcccg ggctgggctt
    17641 gctgcacgta gatattgctt atgggggcaa ctactatgcg atcgtggatg aacaggccaa
    17701 ttataagggc cttgatcatt ataccgcagg acaattgatc cagtggagcc cggtgctccg
    17761 ccagcgcatc aacgaaaaac ttaaactggt ccatcccggg gacccgacca tcggcaaatg
    17821 ttcccatatc ctgtggaccg gaaggccccg atcggaacac gcccacgctc gcaatgcggt
    17881 tttttatgga gataaagcca tagaccggtc tccctgcggt accggcacct cggccaggat
    17941 ggcacaatgg gccgcccagg gtaaactaca gacaggcgat tcgtttgtac atgaaagcat
    18001 cattttcagc acatttaccg gtaaggtgga aggcaccatg gattattatg gaaggcaggc
    18061 gatcataccc agtattgaag gttgggcaaa ggtcaccgga tacaatacga tttttattga
    18121 tgacgatgac ccctacgccc atggtttcca ggttatatag ttttgcagga tgagggtttg
    18181 tgtgatcggg gggggcgtga tcgggctgtt cagcgcctgg tacttggtcc ggtcaggaca
    18241 cgaagtctgc ctggtcgacc ggaataattt taatgagggc gcctcacacg gaaatgccgg
    18301 catgatctgc ccgagtcatt ttacacccct agctgccccc ggggtgatcc gtcaaagcct
    18361 taaatggatg ttcgattccg gcagtccttt ttatatcaaa cccaggtttg acccggactt
    18421 catccgctgg ggcctgcaat ttttcagaaa agccaataga cggcatgtaa aggccagcat
    18481 tggccctttg tccgccctcc tgcaatggtc ctcttctttg tatcgtgacc tggctgagga
    18541 gggtatggac ctgtccttat cccaaagggg cattataatg gcctgcaaag ggcagcatac
    18601 cctcgatgag gaaagccata tcgcaggtca ggccgcggga atagggatcc gcaccaggat
    18661 cttgtcgtcc ggggagatcc acgacctgaa tcccggggtc cggtttaaat tgacaggtgg
    18721 cgtacactat ctggatgata tgcacctggt tcctgaaaag ctgattacaa gcctgaaagc
    18781 cgatttgtcc gggaaggccg aaatgctttc cggcattgaa gtgaccggct ttcaccgcga
    18841 aaacgatcgg attgaccgtg cattaaccca agctggagaa atccgggcag acttttttgt
    18901 tcttgccggc ggggcctgga ccggaaaact ggccagaatg gccggcgaat atgccgccat
    18961 agagcccggc aaaggttata acatcaccat tcctgaagcc aatccccaac tctcaacccc
    19021 cctgatcctg gtcgaagacc gggtggccat cacgcccatg gaccaggccc tccggctcgg
    19081 gggaacgatg gaattgagcg ggataaacga cctggtcctc acccggcggg tcaccgggat
    19141 ccttcgggct attgagtcct gcctgccgga tttcgggatg gaccagactt ccgtgctgaa
    19201 accctggttt ggattcagac cgctcagccc aaccggttta ccggtcattg ccccgctcgg
    19261 gaggctccgg aatgtcatga tcaatgccgg gcatggcatg cttggccttt ccctggcccc
    19321 ggccagcggg aaactgctaa accaattgat cgcggatcac gttagagggg tagcatgaac
    19381 cgggaagcat tcaaactggt gcagccgacc atccctgaat tgcccgggat atataaattc
    19441 ctggatgaga aagggacgat cctctatgtg ggcaaagcga aaaacctgct caagaggacc
    19501 tcctcgtatt tcctggattc gaaaaaccac agttttaaaa ccgtgaccat ggtgcggcac
    19561 gcggtgcgcc ttgaattcct ggtggtggag accgaacacg atgccctcct cctggagagc
    19621 acgctgatca aaaaaaacca accccggtac aatgtcagtt tgaaagacgg ccggtctttt
    19681 gcctttatat gtatcaaaaa agaaaatttt ccccgtattt tttttacccg cagggttttt
    19741 cgcgacggat cggaatattt cggcccctac acttccaaat acaaggcgga aatcgtcctc
    19801 gattttgtca aatcgctctt ccccctccgc aactgcaccc ttaatctcag tccggaaaac
    19861 atcaaaaatc acaagttcaa ggtctgcctg gaatatcatg taaaaaactg cctggggccc
    19921 tgcgaaggat ttgagtccaa ggcggattac gatgaccgga tacgccagat ccggaatatc
    19981 ctcaacggca atttttcgga ggtcaccgct tacctgcagg agcagatgga actctgcgct
    20041 tcaaacctgg attttgaatc cgcccaggcc tgcaagatca ggctggatgc cttcaacgat
    20101 taccagtcca agtcgatggt ggtccatcca tccattggcg acgtggatgt attcagcatc
    20161 gaactggagg atcacgaagg gaccgtcaac tacctgagga tcattaaagg ggccctgatc
    20221 aattcgcata cctttgaact tgataaaaac ctggaccagg acccggaaat gatcctcgct
    20281 tacgccatcc tcgaatgccg ggaacttttt tcgagcattg ccgatgagat catcgtgcct
    20341 tatcctgtgg atctccccgg ggaacggtta aaaatcaccg tacctcagcg gggggacaag
    20401 aaaaaactcc ttgaactctc gcaaaagaac ctccggtatt acatcctggc caaaaaaagc
    20461 gagatcgcca cccatacgac caaacccgtt gcggtccagc ggatcctcaa taccctgcgc
    20521 atagacctcc gtatggaaaa agacccggcg catattgaat gttttgacaa cagcaatatc
    20581 cagggcagcc acccggtagc tgcctgcgtc gttttcaaaa acgcgaagcc tgccaaaaac
    20641 gaataccggc attaccatgt aaagacggtg gaggggccaa acgatttcgc ttccatgtcg
    20701 gagatcgtgt acaggcgcta taaaagggtc ctggatgaaa acaaacccct gcccgacctg
    20761 atcgtcattg atggaggcaa gggtcagctc catgcggcca tggaaagcat catccggctt
    20821 ggccttgaaa acaaagtcaa ggtggttggg atagccaaaa gactggaaga aatctatttc
    20881 cccaatgacc cggtaccgct ttacatcaat aaaaaatcgg aatcgcttaa agtgatccag
    20941 aaaatcagga atgaggccca ccggttcgcc atcctcttcc atcgtgacca gcgttcgcgg
    21001 tcgctcctga gtacaggtct gaccgacatt cccggcatcg ccaaaaaatc agccgaaacg
    21061 ctgcttaaac atttcggttc catggccagg gtacaggagg ccggcctgga agaaatggcc
    21121 cccctgatcg gccgtaaaaa agcggacttg gtctaccggt atttccgtcc cgaggcggaa
    21181 gaagaagaat agcaggcgac tgccctgatc ataaaggctc tagccccaga gatgccgtaa
    21241 aatatatatc ttcgccctgt tttttaatac gaaaagcgca tgttgactgt cgggattatt
    21301 tcaagtaaag gcgaccccag aattgcacta gtaccggaaa cggttgataa atacctcaaa
    21361 ttaccctgta ccctcctgtt tgagaaaggc tgtgggcaat ccgcatccat tccggatgaa
    21421 gttttcggga ccaaagtcgt ttttgaaccg cggacggagg tcctcagtaa atccgatatc
    21481 ctgctcagcc cgctgccccc ggaacgggac gacctccagc atatccgtcc cggtacggtg
    21541 ctggtttcca tgtttgcccc cttccagaac cccggtgtgg ccgaccccct gaaagaaaaa
    21601 caagtctccg cctattccct ggatatgatc ccgagatcga ccctggcgca ggccatggat
    21661 gtgctttctt ccatggcatc catagccggg tataaagcgg tcctcctggc tgcttccctc
    21721 ctgcccaggt atttccctat gctgatgacc gccagcggca ccatcaagcc cgccaaagcc
    21781 ctggtcctgg gagcaggggt ggctggctta caggctattg ccacggcaag gaggctgggc
    21841 gcagtggttg aggtctttga tacccggaaa gcggtgaagg aggaagtcga gagtctgggg
    21901 gcaaaatttg tagaagtgga aggagcaaag gacgaaaaat cagccggcgg ttacgctgta
    21961 gagcaaactg aagaatttct ggcaaagcaa aggcaaatgg tccaggagag ggcagcaaaa
    22021 tccgatgtga tcatctgcac ggcgcaggtc cggggccgca aggcgccggt cctgattacc
    22081 tcagatacgg tggccaaaat gcgcccgggt tccgtcatcg tggacctggc ttcatctacg
    22141 gggggcaact gtgaagtcac ccgggacaaa caaacgatca accatctggg gattacgatc
    22201 atcggcaaca gccagctggc agataccgtt gccatagacg ccagtttttt attgagcaac
    22261 aattacctga attttctcaa aatgctggtg aaagaaggaa aaataaacca cgacgcaggc
    22321 aatgaaatcc ttcgttcgtc ctgtatcacc ttataatatc caatagcctt atgttagact
    22381 ggattcaaaa ccaccaacag atcatctaca tcgtattgct gatggtcttc ctcgggatcg
    22441 aagtgatcgg ccgggtgccc agcgtactgc acaccccgct gatgagcggg gccaacgcca
    22501 tccacggcgt ggtcatcatc ggcgccatca tcgtcatggg caaggcggag ccggacaact
    22561 acctggccct cctcctcggt tttctcgcgg tgatcctggg agcgctgaat gtggcaggag
    22621 gatttgtagt gacggaccgc atgctcgaaa tgtttaagca aaagaaatcg aaataatgga
    22681 gttgagttta cttacgatca tctatctggt tgccgccgtc acctttatac tgggcctgaa
    22741 gatgctttcc agcccggcct ccgcccgcaa aggcaacctc atcgcagcct tcggtatggt
    22801 cctggcgatc ctcggcacta tttttttcta ccgggatgaa gagggcaagg tccttcacaa
    22861 ttacctctgg atcttcggcg gcctggccat aggctcgggc gcgggatggg tggccgcccg
    22921 gaaagtaaaa atgaccgcga tgcccgagct ggtcagctta ttcaacggta tgggaggggc
    22981 ctgcgcggcg ctcatttccc tgattgaatt caatcacctc agccattccg ccggcaatct
    23041 gtccgcggta agcgggggaa ccctgctcat cattttcctg ggactcatca tcggatccat
    23101 atcttttgcc gggagtgtgg tcgcctgggg aaaactcaac ggcaggatta aagatttttc
    23161 attcaaaggc cagcattgga tcaattttat catcctggcc ctggtcgttt tcctcgtcgc
    23221 tttcatcctg atctcaaggc cggaaaccag cgggatgctt ttttatgccg tattattttt
    23281 gtccctcctt tatggtttct tttttgtgat gccgatcggt ggagccgata tgccggtggt
    23341 gatctccctg cttaattcat tcaccggggt tgccgccgct tccggcggtt ttctttatga
    23401 taacaaagcc atgctcaccg gagggatcct ggtaggggct gccggaacat tgttgaccat
    23461 cctcatgtgc aaagccatga accgctccat ttttaatgtt ttggttggca attttggcgg
    23521 aggtgcggca gcggccaccg gtgcagcagc gggaggggcc cgggcggtga aagaaattac
    23581 ggccagtgac ctggccatca atctcaatta ttcaaaatcc gtggtggtgg ttccgggtta
    23641 cggccttgcc gtggcacaag cccagaaaac ggtgaaagaa ctcgaggatg ttctggaagc
    23701 caatggagtg gaagtgcgct atgcgattca cccggttgcc ggccggatgc ccgggcatat
    23761 gaatgtattg ctggccgagg ccaatgtgcc ttatcctaaa ctggtcgacc tggaagaagc
    23821 caatgaaatg ctggctcagg ccgatgtagt cctggtcctg ggggcaaacg acgtggtcaa
    23881 ccctgctgca aaagacgatc ccaccagccc gatatacggc atgccggtcc tggaggtttg
    23941 gaatgccaag ttggtcgttg tagtgaaaag gtctatgggt gccggatatg ccggtattga
    24001 aaatcccctc tttttccatg accggaccaa aatgttgttt ggagatgcca aaaaagtact
    24061 tcaggacctg gtcaatgaag taaaagcctt gtaaagcagg gggaagtgct caggaactaa
    24121 atattcttga gggagcatat ttttcactgc ttttgatcaa aaaagagcaa aaaaccagga
    24181 tttagtaaaa aaggagcaaa aaaaaacgga gaatagagaa ttttatttca atactttccg
    24241 ttaacaataa agtgagagag cgcagcccct gttcgttttt tacaatttga gtatctcagg
    24301 agtaaatttt tctcttctta ttcaggggct gtttttttac ttaacgtttt atttctttcc
    24361 catgctgccc aggagggggt agttttttat tcctttaatt caataatccg gaacctgcct
    24421 tcataaccat agctcaatgt cccggaattt tttaatctga gccgggcatg gagacttagt
    24481 tcctgtccgg aaattgccag tttcacccta cccttccgta ccggtgtttc ctttttgtcc
    24541 tgatagacca ctccaagaat atccctcttt ccaaaagcac tgaatggttt tttcctgaac
    24601 cggtaacttc ccggaaggaa cgatttgcca cgatcggttt tgaccaggat ccacaatacc
    24661 cggtttttat tcaaggcatc atgaaaggtg atttcccagc ggtcggcctc cggtccggct
    24721 tctttatgga tgaccgtagc cgaaccaatg ccgggtgccc ggttcggggc cggctggcta
    24781 ttcagcggat ggatttgcaa gccgctgact gtgaggagca gcaataaccg gattgcgtta
    24841 tagcttctgt tagacccctt gcccatataa caactgtaac ctttacgcgg agtaacggta
    24901 cggacctggt ttattaaaaa catccaggct ttagcagtgg aacaaatact cagttgatga
    24961 agaacatcga atccgggatg tccacattga atttcagttc ggtcacggtc atctccatct
    25021 ggaactgtcc gttgttcaac tgcattttgt gggcaaaaag aatgccatcc accggcttgt
    25081 aatccttcat atcgatcacg gtttcgactt tggttccctg gaattccccg gtggtagcca
    25141 tgcgggacaa cagtccggtg ctggcattaa aatacatttt ggaagtggta ccgtccgcgt
    25201 ccacggccag gatccggtgg caatcctccc cttctatctt ttccattccg tcatgtttga
    25261 gcgtcacctc tccattgatg atggacaact gggggaaaat ttttccctgg ttggcgatct
    25321 gtttgatcaa tttttcatcg tcaatcgtct ggttgccctg catcccgcct ataaaagcac
    25381 gggtaccgtc gtatttttgt tcaaacatga cattgccctg catggtggat ttcatataga
    25441 acttcccatc cttataccag gcctccgact cggagatgcc aaaaggcata ttgatctcca
    25501 ttttcctcgt catggttttg atcgcagaca gcttttcttt ccctccgctt ttcgcaatac
    25561 tgttctgaat gacttccaac gctttagcat cggactggcc gaggacaggc aaagccagga
    25621 gcaggaccag aaacaagagg attgaatttt tcatttttat aatagttaaa gattaattga
    25681 tgaggaataa ctggtccggc aggtcgctgt tgatcatcag agccgtgatt ttcatgtcca
    25741 ggggaaaagg caccgccccg ccggtcattt ttacctggtg gggaaatttc accccgttca
    25801 cttccttgta gtccccgaaa tccatggttt gggtggattt ctgtcccatg gcgtcgaccg
    25861 tggcgatggt ccgtaacagg aggtgggagg ccgcgtcaaa atactgggtg gattcgtttt
    25921 tatcctcatc cgtgaccgtg attttgtgga cggtcttacc atccagttct tcggttccaa
    25981 gatatttttt cagcgacttg gaagacgtga gctgatcgag ttcatcaaac atgcgggcct
    26041 gtttgcgcac tcctttaaga gtttgttcgt ccgtgatctt ctgattgccg cccatgcctg
    26101 atacctgaag ggttgtcccg tcgaactttt gctccattac ggtggaaccg gccatttcgg
    26161 ttttcatggc catctttccg tcctttataa actgggtgat caggaggtcc cccattccgg
    26221 tagaggcaga cattttcatc atgacggatt ttacggaggt cagtttatcg ctgcccccga
    26281 tggcatttat ataatttttc agcacgtcga tggctttttg gtccgattca ttggtttttg
    26341 ccgcaacggg cgtatcgctt ttttccggag gagcctggac cgcagtcgct gttttcttgg
    26401 aacaggaaac cattcctaat gcaaagatga acaggaataa ataaaaggca tttttcattt
    26461 tttgatgttt aagatgaaag gtataagtat gagaaatgta aaagtgagtt cgcagttggg
    26521 attctaacaa aacgggccta ttcagggttc caaattaacc gatttttcta gaatatgagt
    26581 cctcatggaa gactgcattt tcaaggcctc ttcgcgtgcg gcctggatat agtccggtcc
    26641 cgccgaagca taaaggatgc tgcgggacgc gttgatcagg aggtcgccgc tggtggtcat
    26701 cgcctgcttc atcacctggc ccaggtcacc gccctgggct ccaacccctg gtatgagcag
    26761 aaaatattca ggcaacaggt ttctcacctc cagcaaattc ccgatccggg tcgcccccgc
    26821 cacatacatg agttggtcgg gactccccca ccggctggat agatgaatta cttcttcgaa
    26881 cagctttcgg ccggaagcca tccgctgaag ctgaaaatcc tccgccccgg tattactggt
    26941 cagggccagc aggatggccc agcgtcccgg ccgcgacaga aaaggcaata ccgaatccct
    27001 tcccatgtaa gggctcacgg ttacggcatc agcatccagt tgttcaaaac aggcgcgggc
    27061 ataaagttcc ccggtgtttc cgatatcgcc cctcttggcg tccaggatga caaaccgtct
    27121 tttatcccgg atataggcaa tagtatctgc catggcctgc cagccggcct gccccatggc
    27181 ttcataaaaa gcggcattga tcttataagc cacggcaagg tctgccgtag catcgatgat
    27241 ggatttatta aaatcgaaga ctttgtcttt cccggttacc gcccggggga tcctggtttc
    27301 gtccgtgtcc agtccaatgc aaaggcatga ttgaagttcc cggatggccc ggttgagggc
    27361 ctgtctgtcc caaaacgggg tattcatcag gagactgcga ttacggcgct gacctccggg
    27421 aattttgatt taatcgcctg ttcaattccg gcacggaggg taaaagcgga catggaacag
    27481 gattggcaat tgccgagcca cttaatctta accgttaaat cctctgtaaa atcgatcact
    27541 tccacatctc ccccatctac ggccaggtgg ggccgcaatt catccagggc ctgatcgatt
    27601 tcctgaattt ttttttcctt gcttttcctg gtcaagttca ttttcaagtc caaatttaca
    27661 ggaaaaacga caatggccgg ggtcatttgt tcatcctcac cttttcggtt ggaggcatcg
    27721 tttcattccg gatttgcagg ctttcataag cctgcctggc cagtttttca aaaacagtct
    27781 gtgtttcagg atgatgcagg atggccggtt tgccctcatc tccggaggac cgcaccgaca
    27841 taaacagggg gatctgtccc aacaattgcg tttggaagtg cccggcaagt tgccggcctc
    27901 ccccttttcc gaaaatctcg tattgatgat ccggaagttc tgccggagtg aaccaggaca
    27961 tgttttcaac cacgccaagt atagggacct caattccctg catacggaac atgttggcgg
    28021 ctttgactgc gtccgcaacc gccacctgtt gaggggtggt cactataatg gcgccggtga
    28081 cgggtacggt ttgcacaagg gtgagttgga tgtcaccggt acctggagga aggtcaatga
    28141 ccagcacatc aaggtcaggc caaaggcaat cctccagaaa ctgtttcatg atgccggcca
    28201 gccggggccc tctcagcacc acggcttgct ctggttccac tacaaaacca atggacatca
    28261 ccggaatgcc gtgggcctcc aggggaacga tttttgagac cccgtatacc tgcctcacct
    28321 ggggcctttt tcctttgaga cccagcatga taggtatgct cggtccgtaa aggtccgcat
    28381 ccaggaggcc cactttaagt ccgaggtttt tcagtcccaa ggcgaggttc acgctgatgg
    28441 tggatttgcc cacgcctccc ttgccggaag cgaccgcaat gatgtttctg atctgtggaa
    28501 ggttggagct ttttttctcc ccggcgccgg gattgccaaa atgaagatcc acctcataac
    28561 cgggagcgct ttccattaga cccgtggaaa tggcctcatg gagttcggat tttatacggc
    28621 tttcgagctt cggcgggaag gaaaagatga tccctatgcg ccggttttca atctggatat
    28681 tctggatgcg gttggccagg accaggtttt ggccggtgag cgggtccgtg atggtgccca
    28741 gaaactgcag caatgcttcc ctcgtcatag gacaatatta aggtttaaaa aacactttcg
    28801 gacagaccag ccaatgtgat gaatatttcg gatttttacc agtctaaaca ggcattccgg
    28861 caataacaca tggaagtaca ttttggtttg gatgatatcc cttcgctgca acattccgtg
    28921 attacggtag gctcctttga cggggtacac tccggccacc ggaaaatcat cgaaggcctc
    28981 atcgcaagcg ccaaagcttt cgatacccaa agtgtgctga tcacctttca tccgcatccc
    29041 cggctggtcc tcaaccggtt tgaccagacc ctcagaatgc tcaataccct ggatgaaaaa
    29101 atcgaaatct tcaaatccct gggcattgac catgtcgtgg ttgtgccctt caccccggaa
    29161 ttcgcctccc agtcgcccaa ggattatatt gaagattttt taatccggta ttttaaaccc
    29221 aaagcggtgg tcatcgggta tgaccatcag tttggaaaag gaagggaagg cacccgcgag
    29281 atcctcttca gcctgcagga acaatatcat tatgcggtcc gggagatccc ggcccaccag
    29341 atcgacgggg tcaccatctc ttccaccaag atccgtcatg ccctggcgga gggccaattt
    29401 gaccttgtgc gaaaatatct tggttatcca tatccggttt ccggccgggt gattccgggg
    29461 gaccagttgg gacgaaaact gggtttcccg accgccaacc tcatgatcaa cgatacgaca
    29521 aagtttattc cacaacatgg ggtatacgcg gtgaggataa agtggaagtc ctatgacaaa
    29581 cccggaatgc tttacatcgg caaacggcca accacccacg aaagcggccg tttacagatc
    29641 gaagtcaatg tatttgattt tgatggggag atgtacgggg agcaggtctg ggtcgagatt
    29701 cttaaattca tccgccacga cagacagttt gaagacccgg atgccctccg ggctcagatc
    29761 ctcagggacc agcaggagat ccgggaatat ttccaacaag aaacccaggc ctgatgtccc
    29821 ggaccgcaat agtcatactg aatttcaacg gaaaaaaata cctggaacag tttataccct
    29881 ccatcctgca attcagtccg ccggatgtgc agatcgtggt cggggacaat aaaagcacgg
    29941 acgattccct gcccttcctc aggagccatt ttccatcggt cagcattgtt gaaaacgacc
    30001 ggaattatgg ctttgccggg ggctacaacg aagtattgaa acgggtaccg gctgactatt
    30061 atttcctgct gaacagcgat gtccagctcc ggggaccctg gatgaacctg atcagcgcca
    30121 tgaaggaaga tccttggatt gtggcatgcc agcccaagat actttcttac agggaccctg
    30181 aatattttga acacgcgggc gcggccgggg gatggatgga ctggtttgga tacccctttt
    30241 gcaaaggccg ggttctcgac cgctttgaaa aggaccttca ccagtacgac aagccttcag
    30301 aactgttctg ggcaagcggg gcggccatga tggtccgggc ggaatggttt caccggctgg
    30361 gtggttttga cgcagactac tttgcccata tggaagaaat cgactggtgc tggagggtaa
    30421 aaaaatgcgg aggcaggatt aaatccgttc cttccgtgtc cgtctatcat atcgggggag
    30481 gtaccctgcc ttaccaaagt gtgcagaaag tatttctcaa ttaccggaac aacctggcca
    30541 ccctctataa aaatgaatcc gcgggtaagt tgatgctcct gcttccggcg cgtatactcc
    30601 tggactttgt cgccgtcctg gcctttgtgt tgagaaaaaa cgcgaaggcc gcagcagctg
    30661 tttgtaaagc ctattggcag tttatcaaat ggatcccctc gctcaaccgg aaaagaaaat
    30721 cctttaaggc catctgccgt ggaaaagaaa tgaacacggc cggcatatac cggggatccc
    30781 tggtttttga cttttttatc ttgggcaaaa aatcctttga ttccatcgta catgagctgg
    30841 tataaacttg aaatcctgtt tgaagacgag gacctgctcg ccgtcaataa accttccggc
    30901 ttgctgtcca ttcctgaccg gtgggaccag gagaaagagt ccctctacta tatattgggg
    30961 gagagccgtg ccggtttaat ggtggttcac cgcctggaca aggacaccag cggagtcatc
    31021 gtatttgcca aaaaccagga agcccaccgt attctgaacg atcagttttc gaagaatgaa
    31081 aaccggaaaa tatactgggc cattgtggga cagcctgtga gcgaacccgg aacgatcgat
    31141 acccccattg ccgcggatcc ctttgtggca gggaaaatga ccatacaccg gaaaggaaaa
    31201 gctgccctta cgtattttaa gccattggaa aacttccggg acttcaccct cctcgaagtg
    31261 cagattgaaa caggccgaac ccatcagatc cgcgtccacc tgagcagtat tggtcttccc
    31321 ctggcctttg acccgcttta cggatccgac cggcccatca ccatccggga tataaaaaaa
    31381 aggaacctaa gggagccgga agaaaccgcc tcggccctga tggcccgcgt gccgcttcac
    31441 gccttgtcca tcaccctgaa acaccccaaa aaccttcagg aaatgacgat caccgcaccc
    31501 cttcccaaag actttaaggc cttgctgcaa cagttgcgta aatgggggag gtaaggcggt
    31561 caggcgttca tccaggcttc cattttttct tccagcactt ttttcatgga ctggtatttt
    31621 tcaagaacag tggtggcctg ggcgctggcg taaaatccgt ccgaaaacat tttcgtttcc
    31681 atttcggcaa gttcacgctc cagtttttca atttccttct ccaatttctg gtttgatttg
    31741 gtgacctgcc tggcctgcac caccggtatt tttgcaggga cctcgggttc aaccgtttgc
    31801 cgggtaaggg gattggcttt attgagttcc ctgaagtcgc cgatttccct tttggaaagg
    31861 taatactgga tgtcgccgag gtactccttg attcccccgt ttttaaattc tatggtttta
    31921 tccgtgaggt cctgaagaaa ttcgcggtcg tgcgagacca cgatcagggt accctcgtaa
    31981 tccatcagcg cttccttgag cacttccttg gaatcaatat ccaggtgatt ggtaggttcg
    32041 tccaggatga gcaggttgat gggacgaagc agcatacagg ccatggccag acgggccctt
    32101 tcccctccgc tgagcacact cactttttta tccacatcgt ccccgctgaa caaaaaagcg
    32161 cccaggaccg cgcgaagcct cggcctcatt tcgggtgggg agatctcttc cagggtgtcg
    32221 agcacggttt tccggagatc aagggtttca gcctggtttt gggcatagta tccgatattc
    32281 acattgtaac ccggggtcac tttaccggag gtcggaggtt ctgtaccggt caggattttt
    32341 acaaaagtgg ttttaccctg tccgttctgc ccgacaaaag cgatgcgttc gccgcgccgg
    32401 gccacaaaat cgacggattg caggacctgt ttttctccgt aatttttccg gatagccttt
    32461 gtttcaatag agacctcgcc cgatcgcgga gccgggggga accggaccac catgctgccc
    32521 gggtctgtgg ggttccaatc gatccgttca atcttatcca gttgtttgat catggattgg
    32581 gccatcctgg ccttggaagc tttggcccgg aagcggtcga tggttctttc tttctgggcg
    32641 atcagttttt gctgattgat gaaagcattt tcaagcaatt gctcccgttc cgctttctgc
    32701 accaggtatt tggaataccc ggcggcataa tcattgagtt tgccttgttc aatttccagg
    32761 gtgcggtcgg agacattatc caaaaactgc cggtcgtggg aaatggtcag gatcgtggat
    32821 ggcaatgtgt gcaaataatt ttcaagccag atgatcgcat ccatgtcgag gtgattggta
    32881 ggctcatcga gcatcaggat atccgggcca cacaaaagca ttttggccag ttccacccgc
    32941 attttccatc ctccgctgaa atgcgacaag ggctgcataa aatcctgcgg ccggaaacca
    33001 agcccggtaa gcactttttc cgtttcagcc tgggccgtgt ctccgccaat cgtgtgccgc
    33061 cggtgataca ggtcctccag ttcatggagt acatcgccca tgactttttc atccaggtgg
    33121 tcgtgcagcc gggcttccag ccgatttatt ttctcttcaa tggaattcaa ttcttcaaaa
    33181 gccttcatgg cctcgtgaat caggagggtc tggtcgtcaa aatcgatttc ctgccgcaga
    33241 taggccagtt tcgttccttt gggcacatcc accgctcctt tgtcagggct ttgcagtccg
    33301 gctatgattt tgagcagggt ggatttaccc gccccgttcc tgcccacgag agccactttt
    33361 tcgccgtcgg agagggtgac cgagacttca tccagaagga cgcggtcgcc gtattttaaa
    33421 aatatgtttt taagctgcag cattctattt catctgtttc tgatccttgc aggagacagg
    33481 ggttaattcc atgacatgtt ttcgggtaac tcggaaatca ccgaaaaagt atgccctttg
    33541 tcgttcagta cccgtttcca gaggatgttg gattttattt tgaatacata gttgggatct
    33601 ccttcggcga caatccagga gccgacttcc atttcttcgg aaagctggcc cttgacccag
    33661 ccggaataac ccacgaaaaa acggatattg tccgggccga tcaactgcct ttcaatcagg
    33721 aatttcaatt tttcaaaatt gccaccccag tatacgcccc ggattaccgg gacgctgtct
    33781 tccaacaggt cgcctacgtt gtgtataaag tgcagggtat cggtccccac aggccctccg
    33841 taatacaact ccgcctcgat ctccgggaaa tcctccacca tttcattgat tttcatttta
    33901 agcggtttgt tgatgataaa acccaccgac ccgttttttt gatggtgttc gcagagaaaa
    33961 accactgacc gcctgaaatg agggtccagc ataaagggct ctgccaggat gagttttccg
    34021 ctcttgattt tggacgtttt tttggccatg tcgttggatt ctggattcaa atggagctca
    34081 ccggaacctt tcgatcgatt cttttaagaa gtccggaaag cacttttcca ttaccgccca
    34141 cttctacaaa ttcggaaaat ccgtcggaga tcatttgctg aatggtctgt gaccatttga
    34201 ccggggcggt gagttgccgg accaggttat gacgaatttc atccggatcc cggtggggag
    34261 cggcatccac attctgatat accgggcaag cgggacttga aaaaacggtg gtttcaatac
    34321 cggccatcaa ttcgtcttcc gccagtttca tcaacgggga atggaaagcc ccgcccacgg
    34381 caagctgaac tgcgtttctg cagccgagtt cccggaggtt ctgcatggct tgctggaccg
    34441 catcgatctc tccgctgatg accaattgcc cgggacaatt gtaattggca gccactacga
    34501 cgcctttggt ttcggcgcag accttttcaa ctacagcatc ctccatgccc acgatggctg
    34561 ccatggtgcc gggggtaagg tcgcaggcct tttgcatggc cagcgcacgg gtgtaaacca
    34621 gccttaaacc gtcttcaaaa cgcagggccc cggccgccac cagggctgag aattcaccga
    34681 gggaatgacc ggccaccgcc tcaggtctga agtcggccgc cacggtggcg gaaatcacag
    34741 aatgaaggaa gatggccggc tgggtgatcc gggtctgtct caggtcttcc tccgtcccgt
    34801 tccgcatcac ttcgaaaata tcaaagccca ggatttcatt ggcagtgccg aatatttcat
    34861 tggcctccgg gtagccggcc agtttgctgg ccattccggc aaactgggcg ccttgtccgg
    34921 gaaaaagata ggctttcacg ttttggtcgt tttgaagttg atcaggctga ggaattcgga
    34981 acgggtttcc gcattcctga actcaccggt aaatgcggaa gtcgtggtga tggagttttg
    35041 tttttggacc ccgcgcatca tcatgcagag gtgcatcgct tcaatgacga tggcgactcc
    35101 caggggttcc agggtgtcgt tgatacaatg caggatctcg tcggtcaacc tttcctgtac
    35161 ctgcaggcgg cgcgcaaaga catccacgat ccggggcagc ttactgagcc cgacgatcct
    35221 gccgttcggg atgtaggcaa tgtgcgcctt accgataaac gggagcatat ggtgttcaca
    35281 gagggaatac agttcaatat ccttgaccag gaccatttcg gaatactttt cttcaaacat
    35341 ggccgaccgg agtatggccg ccccattttc cgaataccct ttggtgaggt attgcagggc
    35401 tttagccacc cgctcggggg ttttgatcaa gccctcccgg gcaggatctt cccccaatgc
    35461 acgcagcacc tcctggtaat gcccggtaag cgtttcggtg acggctcgat cgtaggtctc
    35521 attcttttta taggccatgc cggatggtct gtatttgcga gtgcaaaggt acatacttat
    35581 cccatgccgt ttggtctgcc gtaaattatt gttggtctat attggggaag gggtgcgggc
    35641 agaagggctt atctttggga tattgatgga gatgccaacc ctatccctgc caaaagtatc
    35701 catttcaaga ttgtggctgc acggcacaat ctggatgatc ttcctggtcc tcctggtcct
    35761 gatggacaac aacccataca gcctgggact gtccttcctc aacaacggga gccggctgat
    35821 gctgtacggg ctgctgattt attttaattt caactacctc attcccaggt acctgaacac
    35881 caataaactg gccatctatt ttggaagcct ggtggtcctg gctctgatcg ccaccccgct
    35941 cggcaccctt gttcaatatt tcctcctgtc ctcctatccc cagctgcagg gcaatatcca
    36001 gtcccagcag gggccattgt ttctggtctc catgatgttt ctcatttttt caaccagcgg
    36061 caagatcatt gtggactggt tcaaacagca gcaaaccatg agggacctgg aaacccagaa
    36121 catgcagtcg gagctcaaat tcctgaaaac ccagatcaat cctcattttc tgtttaatac
    36181 cctcaacagc ctctacgccc tgaccctgaa gaaatcggaa caggccccgg agatcgtggt
    36241 caaactttcg gaaatgatgc gctacatgct gtacgagtgc aatgaacgca gggtcccgct
    36301 tgaaaaggaa gtgaaataca tccagaatta cctggacctg gaagccctcc gccaaagcaa
    36361 cctggtggac atagatttta aggtcaaagg ggatatcagc aaacacaaga tcgcccccct
    36421 ggtttttatc ccattcctgg aaaacagttt caaacacggg gtcaatcatc agctatccga
    36481 cgcctatgtc tatgccaccc tggccgtgga gggcgatgaa ctggatttca agattgagaa
    36541 cagccggtca accggtctgc agaacctggg accgaaaccc cagggcggga ttggactgat
    36601 caatgtccgg cgcaggctgg aattgatcta cccggggcac cattcgatcg atatcgagga
    36661 ccaacctcat acatataccg tacatttaaa gttaaaatta acaccataat tttcatgctg
    36721 aaaaccatca tagtggatga cgagcccctg gccctggacg tcctcgaaga gtttatcaaa
    36781 aaagtccctg agctcaaact ggttaaaaaa tgccagaatg ccctcgaagc cggcgaagcc
    36841 attcgtaaaa atgaggtgga cctgatcttt ctggatatcc aaatgcctca gatcaccggg
    36901 atcgaattta tccgctccct acccaaaccc cccaaagtca ttttcaccac ggcctacagt
    36961 cagtatgccc tggagggatt tgaactgaat gccgtggatt atctcctcaa gccgatttcc
    37021 ttcgaccggt tcctgaaggc ggtcaacaag gccctggata ccgggtataa gcaggaaacg
    37081 gaaacggagg tccccgagga tgggcctgat tttatattcg taaaagccga taaaaaactg
    37141 atccggctca agtacagcga aatccagtat gtagagggac tcaaggatta tgtgatcatc
    37201 aaaaccgaca cgggcaggat catcacgctg caaaccatga agagcctgga ggaaaagctg
    37261 ccccagccca ctttcatgcg gatccaccgc tcctatatcg tcaacctgga gaaaatcaac
    37321 gccgtagtgg gaaacatggt cgaagtcacg gaaaaaggtc agcccaaact gttgccggtg
    37381 ggaaaaaact accgggaaga gttggaaaag gtcatcgata agaacaggct gtaaggaaat
    37441 ccatcaaata aaaaaggctt ctcccttgcg gaaaaagcct tatgcattgc tttaattttt
    37501 aattaaaatc cggaacgctc ggtccttttc ttagctgaat agttgacttt cagctggccg
    37561 gccttgcgca attccgtttt tacatcgctg acaaacccca tggtcacatc gccgtcgatt
    37621 ttcatggaag tcgtgattcc gggacgctga ccttcgggca gttttacctt atgcctttca
    37681 agaaacaaag ggatttcatt caggtcggca atcttgtcat tcaactgtaa tctgggtttc
    37741 gtaccgtact gatttacaaa ttgagaaaga ggccttccga tgtacatggt attgaccagg
    37801 gattttttct ccagtttggt cagttccgta gcataaggag tgacggcttt taccttgaga
    37861 ttggattccc tcagcttcac gaccaccatg aagaaaaaca ggagaataaa tatgatatca
    37921 ggcagggacg aagtggaaat cgtcggcgcc gtatcggctc tttttgttga aaatctgctc
    37981 atttcctggc gtttttctat gaattaccaa tcatttttct tccccgaatg cggtaggctc
    38041 agcctcagac agcacaaagg ggatgtccct tttaatttca ttttgctgtt ccttattgca
    38101 ttcttctatg gtgtctttcc tgaatcgctt tttggcttct tcattccaaa gttcgctgta
    38161 agcggccttc agttcattgt agacggcgag atactgtttg aaatgggtcc cccggtcgtt
    38221 tttcaatgaa atgatagcct cctttgccga ttcagccagg tcttcccggc ggcccgggtt
    38281 tgagataaat tctttagccc tggttttgag gtcttcgatt ttcaccggct ccccccttac
    38341 caggagttga tcctgggcat ttaccaatac ggtaaacacg ttgcgggtat tcagggtcag
    38401 gtcaatcggc ggctcctccg accattgcgg gaggcgcacc agcacccctt tgtcctccac
    38461 aatattggtc gtcaccagga agaagatcaa cagcaggaag gcgatatccg ccatggaact
    38521 ggcattgatc tcaggatttg cttttttctt ttttttggcc agcatggatc cttttgattt
    38581 tatttgaaca aattccgtat ttcggagaat ataaagatag ccaccgctcc caacaacatc
    38641 aggagcatca gccatatccc ccctcctaaa agtttgctca tgtttgcaga gacctcattg
    38701 gttttgaggt catccaggat agcctggttg tcgggcgcat tcaaaacaaa atacccaagg
    38761 gcaaacaagc ccacgatcac agccagccag atcaaaccca ttttggctcc ggctggattc
    38821 atggcgacgt ggatgacgga ccacaatacg gtgatgacca ctgcgaccac caacatgagc
    38881 aacatgatca cgatacccat gttgaaaata ttgctcgttt tttgttgttc cgtgggaagt
    38941 gcattgaaag aatcaagccc gttcaatacc ggtacggcaa aaatgatcat ggccaccaaa
    39001 gcgagggcaa atgcgaagaa ggtccctttc cctgaaagca gtttatatag attaaccatg
    39061 gtctgatttt tattttattt aaagcctgtt aaacaaatgg aagaccctta tttaaatatc
    39121 ttgttcttgg tcaggatatc caccaggcca atgctgctct cctccatctt atgcgtaatg
    39181 gtatcgattt tcaccacgat gtaattgtaa aagatctgca ggataatggc cgaaatcaaa
    39241 ccgaagatcg tagtcaacaa ggccactttg ataccgccgg caacgatggt cggagaaata
    39301 tccccggccg cttcgatccg gtcgaaagcc tgcaccatcc cgactaccgt tcccaagaac
    39361 ccgagcatcg gggcaagggc gatgaacagg gagatccaaa ccagtccacg ctcgagcaag
    39421 ctcatctgca tgcctccaaa agcggatata gccttttcca cggcatcggg gcctttttct
    39481 gcatgccgca atccttcaag a
//
LOCUS       DAOQXT010000037        39261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1188_length_39261_cov_6.094067, whole genome shotgun sequence.
ACCESSION   DAOQXT010000037 DAOQXT010000000
VERSION     DAOQXT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 39261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39261
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1188_length_39261_cov_6.094067"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..820)
                     /locus_tag="PKM27_08390"
     CDS             complement(47..820)
                     /locus_tag="PKM27_08390"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR07319.1"
                     /translation="MAGLEQKVASAYAAYRQDSLDYERYKRLNEQDIGSVQILDQARL
                     RYQTSQSNFMIATRAFQETRGQLRIELQNANNNYLAQKSLLGEYMLVAAISGKVYDIV
                     PKAGELVSSNHAVIDLGATDSFEVEMLVDESDIYLVKQGQPVYFELVALEDTVFHGTV
                     KNIYPRINPVDKTAKVVASIDPLGCDLYPGMSLEANIIIRQKKGILAVPVEYISMDGT
                     VIVKKGSKKETVKVRTGIRDLEYAEILEGLEENDHIIKP"
     gene            complement(860..1138)
                     /locus_tag="PKM27_08395"
     CDS             complement(860..1138)
                     /locus_tag="PKM27_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNR07320.1"
                     /translation="MNFHSATLLWIVLLLFSCDDREEHIIRRKDLVQAVYASGEVLPV
                     GFYEVTSKIPGIVDTIFVSVGQTVKAGDALLRIQSQANRLNLQTAKNL"
     gene            1448..3904
                     /locus_tag="PKM27_08400"
     CDS             1448..3904
                     /locus_tag="PKM27_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNR07321.1"
                     /translation="MKQESSLLLPVLILFGSSLFAQQASIAEKINGKTFDPSTLLWYD
                     TPAQKWDEALPVGNGRLGAMIFGKNGEERIQLNEETYWSGGPYSSVVKGGYKVLPEIQ
                     KLVFEEKFLAAHNLFGRNLMGYPVEQMKYQCLGNLHLFFKNQDSVTSYKRWLNLEDGI
                     SGVAYSANGVNYQREVFASAPDQVIVVRITADKPGRISFTANLRGERNQAHSNYGTDY
                     FQMNPYGDDGLVLTGKSTDYLGVEGKIGYEARIKAQPEGGTIKTDGVDLVIENANAVT
                     LYFTAATNFVNYKDVSGNPHQRVNQYFQRLDGKSYPAIALAAAADHKKYFSRVSLQLP
                     KTANSYLPIPERVIKIQTEPDPSFAALSYQFGRYLMIASSRPGTEPANLQGIWNNDMN
                     PSWDSKYTTNINTEMNYWSVESGNLSECAEPLFRMIRELTDQGSQVAKEHYGARGWVF
                     HQNTDLWRVAAPMDGPTWGTFTVGGAWLCTHLWEHYQYTMDRTFLMEAYPLMEGSVQF
                     FMDFLVPHPNGKWLVTNPSTSPENFPDGGGNKPYFDEVTAGFREGTSICSGSSIDMQI
                     LYDLFGYYLQASDVLRKEDSFVQKVKRAREKLVPPQIGKEGSLQEWADDWKSLEKNHR
                     HYSHLYGLYPGKVLFEKRTPALVEAYKKVLEERGDASTGWSRAWKMALWARLNDGNRA
                     NKIYKGYLKEQCCISLFALCGRSLQVDGSFGVTAAVTEMLMQSHDGFIKLLPALPDEW
                     IEGSFRGVCARGAFELEFSWHNSILSHLKILSKAGEVCRLEWKPGLKITSNGNPVVYN
                     KHSNGLAEFQTVKGTVYQVQ"
     gene            3934..6165
                     /locus_tag="PKM27_08405"
     CDS             3934..6165
                     /locus_tag="PKM27_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNR07322.1"
                     /translation="MKRSILFTSALLIAVLCGAGLFAQNKELTLEDMYIHNHYRSKGI
                     GPLRWMKDNKSYSTLEFNDALQGNDIIRYDVAEGLRTVLVHARQLLPAGAAKALAIED
                     YAWSEDNSKLLVFTNTRKVWRYHTRGDYWVLNLTDGRLRQLGKGLGEATLMFAKFSPE
                     GGRVAYVSKQNIYVEDVATGKITPLTQDGGGNIINGTFDWVYEEELDDRDGFRWSPDG
                     THIAYWQSDTKGVGTFYLINNVDSNYSVPIPLPYPKVGTPNSAVKVGVVPATGGKTRW
                     FAVPGDPRNNYLARMEYIPDSDEVMLQQLNRLQNTNTVWVGNTKTMALKNILTDKDDA
                     FLEIHDNVVWLDNHRFFTWTSEKDGWLHLYKVSRDGKQMQCITNGDFDVIQVNCIDPE
                     GGYVYYTASPENFTQRYLYRSRLDGSGEAERVSPMNMAGQHAYQISANAKYAIHSFEN
                     AYTPRRISLIHLANHAEMKMLEDNALLKSKINDLGLRPKEFFKVDIGEVVLDAWVIKP
                     RSFDPRKKYPVLFHVYGEPAGSTVQDNWGTGDNLWHQYLANELGCVVISVDNRGTKVP
                     RGREFRKCIYRQIGLLAADDQAAAAKKIMNMYSFIDPARTGTWGWSGGGQMSLHGIFR
                     HGDVFKTAIAVSFVAHQTLYDNIYQERYMGLPEDNPDGYREGSPVTHANKLQGKLLLI
                     HGTGDDNVHYQNFEIMVNELIKHNKLFSMMSYPMRDHGINQGINTTLHMRRTMEQFWK
                     KNL"
     gene            6184..7761
                     /locus_tag="PKM27_08410"
     CDS             6184..7761
                     /locus_tag="PKM27_08410"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNR07323.1"
                     /translation="MVSYPSIFNDVIGPVMRGPSSSHCAAALRMARLCRDLMDENIKD
                     ILIEFDPKGSLATTHKSQGSDMGLFGGFLGWEAHDERLPEAENHLITAGIQVSIRIHD
                     IGNHHPNLYQITLSNPKETRKLTAISTGGGMIEVTAIDDLPVSMAGDYYETLIYCNQP
                     DPVLSFLKASVVYDELEVHQGAFCFIEIKSQAFLDSAIKKELQEMEGVLFIKQLHPVL
                     PVMARKNLQVPFITCNEMMLYNKDRNKMLWEPALDYEMARGNISAEEVMEKMRSIVRI
                     MEQAVHSGLRGTQYEDRILPSQAPHYQKQFKAGKLAGGEVINRIVMYVSAIMEVKSSM
                     GVIVAAPTAGSCGALPGALIGMAHSLHLSEDELVKAMLSAGLIGVFIAAHATFAAEVG
                     GCMAETGSGGGMAAAALVQMQGGHLAQSIAAASLALQNSLGIICDPIGNRVEAPCLGR
                     NVMAASNALSCANMALSDYEHLVPLDEVIDTMKAVGDQIHHTLRCTNLGGLCVTPAAK
                     AIEKKLEQQQLSFYKSC"
     gene            7910..9010
                     /locus_tag="PKM27_08415"
     CDS             7910..9010
                     /locus_tag="PKM27_08415"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNR07324.1"
                     /translation="MRFLSLDVFRGMILVLMLIVNNPGDWGAVYAPLLHADWHGCTLT
                     DLVFPFFLFIVGVAVPFALGRRKESGSSGMELYKKIFFRVLLIFGIGLALNGFPYYHF
                     STIRIPGVLQRIALVYGVIAVLYLKCSTRVLFYILVTLLLAYWFVLTQIPVPGYGYAS
                     LEPETNLGAWIDYSLLKGHLWSGSKTWDPEGLLSTLPAIGTGLLGIFAGNWLRRDGNP
                     AEKINILFVTGGLCLLLGYFWDMVFPVNKKIWTSSFVLFTGGLALLTLATLTWLIDVR
                     GYKKWTIHFVYFGMNSMMVFVGSGILARLLGIIRWNQGEKMVSLKGFLYSGLQATGLP
                     TKITSFLFALVFAFLFYLLLKLMYHKKVFWKL"
     gene            9075..10109
                     /locus_tag="PKM27_08420"
     CDS             9075..10109
                     /locus_tag="PKM27_08420"
                     /inference="COORDINATES: protein motif:HMM:NF023431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HNR07325.1"
                     /translation="MKKLIPSLLMVFLFAGNALTAQQQMPMLSPGEAAADLLKSLRPD
                     QLDKMSRPWDDKERVNWDFVPKEYRAGVTVGSLSDGQKEKFYRLVLATGGEKTLDRVK
                     SISFLESILRGVEGRKEGDAYRDPGKYFIQLFGATHLDKTWGWKIEGHHLCFNYVLEK
                     NKVIAASPSVLGANPAIVLEGENKGYQLMKPEIQLATSILSSLNTGQKTKAIVSSQAS
                     KEILTYQQRKASIDKAGGVSFTELSPAQQTKLKELVELYIHRASRFFVKDMLARVEQA
                     GWNKVFFVWEGSEDVSPGHTHYYRIQGPEFIIEYDNYQNNGNHVHSIFRDVRNDFGDL
                     LAAHYKEEHP"
     gene            complement(10088..10756)
                     /gene="mnmD"
                     /locus_tag="PKM27_08425"
     CDS             complement(10088..10756)
                     /gene="mnmD"
                     /locus_tag="PKM27_08425"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNR07326.1"
                     /translation="MLFEVFRTDDGSETVMQHPLGVSYHSRFGARTESEQVYLVNGLH
                     FFTSENPGLSSVKIFEMGFGTGLNALLAAQYAQKTGIGIEYHTCELYPLPAEIYTQLY
                     RGEAALLDPLHEAAWDKPARIHPFFTLVKYKQDLRQLDLTEVFDVVFFDAFDPDSQPD
                     LWDYTVFEKLALAAHPGSVLTTYSSKGSVRRNLEKAAWRVEKLRGPAGKREVVRATQG
                     CSSL"
     gene            10843..11919
                     /locus_tag="PKM27_08430"
     CDS             10843..11919
                     /locus_tag="PKM27_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR07327.1"
                     /translation="MKSVHKVQIQLSTPDPAGEAVCISGSFNRWEENEYKMKEIRPGE
                     YQLDLELNVPEDHPVEYKFIRSRWANVELDEFGSFTENRILTDPDEPVRAWVPRWRNE
                     GRVCDISLIPQVEIHHDMVITGTRKKRRVSVLLPYDYAYTDKRYPVLYLMDGQNLFED
                     HAPFGSWEIHKKLAVMAEKNMHEVIVVCIDHAGLKRIEEYTYIRHNSTEKGRGHLFLD
                     WVVGRLKPFIDKHYRTKPEPTNTGIGGSSMGGLIALMAGINQPRYFGRLMLFSPSLWK
                     ILDLIYDRLIYLPLISHDQFIYLYAGGKEASNMKGFALDFFDQLIRTDPSNDRVHLSI
                     HEAGEHSEKYWGKEFPYALKYLFF"
     gene            11931..13406
                     /locus_tag="PKM27_08435"
     CDS             11931..13406
                     /locus_tag="PKM27_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HNR07328.1"
                     /translation="MRPIPIYSPEDMRQADYILIPVFKKDHKKLLKYLAACEGQLPSP
                     WSKQALLLAENHEPDYKKFIEQWVGKQKLILMGLGDERAYQKIAGVCRLAVHKQEEKP
                     SGGKIVLVLPDYLDGNSISALANGLALSAIRLDYYKSEKKGKPAELAFFTGKKSTLKS
                     IEAGIRIASTQEEICHLVNLPPNEINPQYLAGWARRAFHGRPVKVEILEEEALAQHKM
                     GALLAVGMGSETPPRLLVLDYQPSTRAKKLKHIGLVGKGVTFDTGGISLKDPANMHLM
                     KSDMGGAAAVLGAVDLAEKSGLAIRLTAVIPLAENAIGPASYRPGDVIHSYSGKSIEI
                     IDTDAEGRLILADALAYLVKKYKPEIMVDLATLTGSCIMTLGNKAAGLFTNNERLAQG
                     ITAAGEATGERVWRLPLWKEFEEEMHSDIADIKNLATKPVAGASTAAKFLEAFTSGHP
                     AWAHLDIAGVAMVDSDFSKMRSATAYGVHLLVKWMEEMIKP"
     gene            13379..14626
                     /locus_tag="PKM27_08440"
     CDS             13379..14626
                     /locus_tag="PKM27_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNR07329.1"
                     /translation="MDGRNDQALNMESPLTFLCIASYFKGNRFMQALKQCGARVFLLT
                     SKKHEDKAWPRESLDEIFYMESDERNHWIMEDAVKGLAFLMRREKIDRIVALDDFDVE
                     KAALLREEFRIPGMGQTTARYFRDKLAMRLRAQATGVAVPAFTPIFNDEMVNGFIRDT
                     PGPWMLKPRGEASATGIKKIETAEQVWKQIQELGEERHQYLLERFLKGQVYHMDSIVQ
                     DGMVLFCRCSQYLAPPFEVAHGGGIFRSVTLEPEDEDNRALLRLNEDILGGFNLRSSA
                     AHTEALKSEEDGKFYFIETSARVGGAHLAEMVEFATGINLWEEWAKVEYTAALYQPHL
                     LKSWDPGHAGILISLARHKSQDYSRISDPEIVWTMTDMDHHFGCIVKSHDRRRIIELL
                     DQYTALVQAEFHATAPAPDKPIH"
     gene            15015..17111
                     /locus_tag="PKM27_08445"
     CDS             15015..17111
                     /locus_tag="PKM27_08445"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNR07330.1"
                     /translation="MDLKAAGPGSGGAYRMNEMMIRDLMASDRVSRQDPTGDLMALVG
                     DAAQWIRLKGEMAFDDFRVSGSRWRQEETYIFVLDPSGNMLVHNDPELEGKNQLALKD
                     INGKHIIRGLLDAVNAIPDKPEGWYHYQWPVPGGLLPRWKSSYVQKVKAPSGKSYIVG
                     SGTYNDRMEREFVVDLVKRAVNEINKQGKAAFKLFHDPSGPFLAKDAYIFVITMEGIE
                     IVNPAFPSIEGRNNLDVRDPQGKYLTREIIQVAQSRGTGWVDYMWPKPGESVSTQKSA
                     YVARAILDGKPVAVGCGVYLGETPKIIAPANKMTATELMALVREGAAVLEEKGEKAYP
                     EFRTKGSRWYRDDTYFFVNTMEGIRRFHATEPSSEGQNVVGDKDVLGRPIGKMSLEAA
                     GSASGEGWIHYMWPEPGDIFPKWKSTFVKRVTFPSGKQYLIGSAIYQLQMDKAFIEDI
                     VDRAAVLVAERGKGAFDLLRDKKGPFMFMDTYVFVNNTVGVELVNGAQPSLEGKNVIN
                     ERDLNGKRAVYDYIEAALKNGSAWIDYYWYRPGGNEPARKHTYVRKVQHGNETYIVGS
                     GFYEVDAGQETGEARKHSWNAIEKEEMSKSLYRQAISGEKGTLSQFSAKGGARIALHS
                     HPSEEYFVVTSGSVKYSVEGREYIIMPGEMMITPSQVPHEIEVLEETVFVVFFTPARE
                     DWLRGADHYLRYQENK"
     gene            17545..19632
                     /locus_tag="PKM27_08450"
     CDS             17545..19632
                     /locus_tag="PKM27_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNR07331.1"
                     /translation="MSIIEQATVLEKEFTGTGMINAPSNSGFTVAKDSTPEIISLVSD
                     AADLIRLKGEAAFDEFRRPGSRWRMEDTYIFVLDPSGNMLVHADPQMEGRNQLALEDI
                     NGKPIIRGLLGAVTNLSDKREGWYHYQWPVPGGLLPRWKSSYVRLVHSPTGKSFIVGS
                     GVYNDRMEREFVVDMVRDAVGQIEKFETASFRLFHDPKGPFMAKDAYIFVINEQGVDL
                     VNPGFPNLEGRNIMDLKDTRGKYLIREMFRLVEAEGAGWVDYMWPKPGESVSTQKSTY
                     VTRARMGNQSVLVGCGVYLADAPKEPALTKKMDAAELMALVREAAVIFEKNGEKAYPQ
                     FREKGTKWYKEDTYFFVWNMEGIRVFHAAEPAGEGQDVSKLKDIHDRPIGYMFLDAAS
                     TGAKEGWVHYMYPEPGDIFPTWKSTFVKGVTFPDGKPYLIGCGIYQMEINKSFVEDVV
                     DRAAKLIAKQGKQAFPQLRDKTGPFVFMDTYVFVHDLQGTELVNAAQPSLEGRNLLDL
                     KDLSGKEVMKEQLEAVRNAESAWLDCYWFKPGDNLPALKKTYLRKVRFGPETYVVGSG
                     LYLSGDEGKPAGIRKVSWKDLETVDFSERLSRQMISGEKGTLARFNAKKGVGVDRHQH
                     DNEEYVCVLSGVMKFIFDDREVLLKKGDVLIIPANVAHDIEVTEDADFFDFFIPLRSD
                     WVRGEDQYLRKEN"
     gene            19802..20209
                     /locus_tag="PKM27_08455"
     CDS             19802..20209
                     /locus_tag="PKM27_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07332.1"
                     /translation="MCPRYEYGLGLTLFLLFTGCVEEARWNQDIFVLYHIHKIKSLSM
                     IRLPEDYRIKSFDVLDDHSSYQVKFRLDYHENQIRDMSDRFRQIRRESSGPGWGKYPA
                     GWEFFHQPLLDEQFILRIDTLAGEVEYSYMLLY"
     gene            complement(20289..21686)
                     /locus_tag="PKM27_08460"
     CDS             complement(20289..21686)
                     /locus_tag="PKM27_08460"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNR07333.1"
                     /translation="MKPQLTFYRWGAGLLFLIFAPALQAQTFPKDSIRWNGDLKKFIN
                     LVFLGDGYQAGDLNKYQEHVGNTADYLFKFSPFKEYADYFNVYAIKVPSAQSGADHPR
                     TAVDCVPAAEHPLLVTENYFNSTFDYSSIHRLLVPTNPGAVINVIIDNLPLYDQPLML
                     VNSTFYGGSGGSTAATASINTSSFEIMVHEIGHSFAGLADEYWVNEFSAGERANMSRE
                     KDPAKVRWKNWIGFNGVGVYPHGASGGQENWFRPHESCEMRFLNSEFCPVCKEAIVLR
                     IMQHFGTPVLSRFPADPSVTLMADSIPFKVDLQKPNPNTLRTKWILNGQLIFMNRDSI
                     LLRPNQLLPGNNTLTFEVLDTTPLIRADNHPTLNTHVLNWNIQLASTGHDDASAWQGL
                     RVYPNPVFNELTLSMDGNPEKLKFEIHNILGQIVFSGYLKDMIKVNTHAWIPGLYMLK
                     IENGNDYTSRKILKQ"
     gene            complement(21706..22185)
                     /locus_tag="PKM27_08465"
     CDS             complement(21706..22185)
                     /locus_tag="PKM27_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07334.1"
                     /translation="MKVLTFLALVISLPFLACSKKTQAPPEQESGKPRTLLLSLRIEK
                     KGNDYQVTIPNSRIIDAPFMGNEPGPVIYRENDFLCFIQDRDKKNLDTLIVSQPLHPR
                     YEFPGEEGAIGSKQVELQENEVLLRFAYTSRMTYLQVHKVEKNKQLKTLNTLKLPGN"
     gene            22341..24728
                     /locus_tag="PKM27_08470"
     CDS             22341..24728
                     /locus_tag="PKM27_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNR07335.1"
                     /translation="MAKFFFLAMMGFTLWTAGLRAQSAQSLLNEPLDLSRDFRDYSNT
                     YFLADSLASFDPAKASGQVLWRRAEYFPAHAFNYTQHGIRRTVQNEFPAREYAADPVW
                     PFQLEFITPKTVRIRMNTGVSRVETDTLMIPGGRVASDKSWIYTRIEGGHQYKSQQGM
                     VRIYEQPWGVEFFDHEGRPLTKTRHSSDNKGFCQTMPFCYVRRAADYSRSVGAVFTLS
                     PDEKIFGCGESFTKLNKYGQKVNLYTCDPNGVETPGMYKPVPFFMSSRGYGMFMHTST
                     PITCDFGNSYGASNALWIGDEALDLFVFMGKPRDILDEYTRLTGKAPMPPLWSFGLWM
                     SRITYFSEADGRNVAAKLRANRIPTDVLHFDTGWFETDWRCDYEFSKNRFPDARGMIA
                     DLDKDGFKTCLWQLPYFVPKNKLFPEIVDQGLHVKNAKGNIPFEDAVLDFTNPRTLDW
                     YREKIGGLLRMGVAAIKVDFGEAAPFDGIYSNGRTGFYEHNLYPLRYNQVVSDLTQQL
                     RNERIIWARSTWAGSQRYPLHWGGDAETSDMGMEAQLRGGLSLGLSGFTFWSHDIGGF
                     TRRTPENLYRRWLPFGALSSHTRCHGQPPKEPWDYGEDFQNYFRKVIEIKYSLMPYIY
                     TQSLMSSQAGLPMTRALLLEFPEDRGAWEIENQYLLGSDILVAPLFEEGRLSREVYLP
                     AGKWIDMQDGRSYSGGWHRMSAGAVQAIILIRDGTVLPRLKAAQSTRDLDWSQVDLVV
                     FSTSSEAKGMLAVPGETEIRNLKLIKKKKGFELAQEGLPVNVNFKIVPFEQALIK"
     gene            complement(24962..27130)
                     /locus_tag="PKM27_08475"
     CDS             complement(24962..27130)
                     /locus_tag="PKM27_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNR07336.1"
                     /translation="MSISSLSTERRRFLKQSLLGTAGLMIGVRAHAGGHALVKLFENN
                     ISTELNPFVLIDAKGVITLVNSRPDMGQGSIQAVTSLIAEELEVRMDQIQIVHSDGQA
                     KYGSQGSGGSSTVRGLWFDLRKAGAAAKEMLVRTAAGRWNTSPESCQARDGGVFLTGT
                     DKKFSYGELAVDAAKLDVPKNPKLKDPKDFKILGKYHKRSDIPSKVNGTAVFGIDVVV
                     PGMLYATVIHAPSVQGKLVSYDATQAMAVAGVKQVIKTEHSYPGGKSEAIAVIAENYW
                     AALKASKLVTAQWDDGDLVQTLNTGQYFKDAYAAAAKPGVRAEEDGDFDAFFAASEKK
                     LESIYETPFLAHAAIEPVNATAHVKEDGSVEVWAPIQGPDGALGETADYLGIATDKIK
                     IHPQLLGGSFGRKAFMDFMKEAIDLSRQVKAPVKLIWSREEDMMQGPFRPAMLSRMRG
                     VIGNNRLSALSHQAIGESIQGQHWNGLADYQADDWLAGELSKENHKYGFPVHHLSYSR
                     VKTPIPILWWRSVYASNFAWGQECFIDEMAHKAGLDPLAARKQLLRNSKRDLAILEKL
                     EEIIKSSPALPTGHARGIAMFRSFESMSAAMVEIARKDKGARIVKVTSIIDCGQYVNP
                     DNVKAQTEGNIAMGLSSAIKGGITFSEGKCDQTNYHQFQVMRMSEMPEVEVHIMDNLE
                     NPGGVGEPGLPPIAPALGNAIFNLTQKRIYKLPVDLDNLV"
     gene            complement(27175..27663)
                     /locus_tag="PKM27_08480"
     CDS             complement(27175..27663)
                     /locus_tag="PKM27_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR07337.1"
                     /translation="MAIFNLTINDQRYKIEAEEDMPLLWAIRDLVGLKGTKYGCGIAQ
                     CGACTVHLDGQPVRSCSLPLGVIKKKQKITTIEGIGSPQLLHAVQEAWVEEQVPQCGY
                     CQSGQIMSAVALLAQHPEPSDDQIDTYMNGNLCRCGTYDRIRKAIHRASGSAKPKTMG
                     TH"
     gene            complement(27817..29919)
                     /locus_tag="PKM27_08485"
     CDS             complement(27817..29919)
                     /locus_tag="PKM27_08485"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNR07338.1"
                     /translation="MTKTIWKKTVSVFICFSVLFTLPALRYSDAPGDKYDQTLHTLAK
                     VIQQGHFSPRPFNDELSSEVFLEFIKRMDSEKNIFLQSDIAAFEKYKDLLDDELNGQT
                     VQFFYEVLARYKERVAELSREFPSWFEKPFVFAASPDEWYDPGKSYPSTMAERSRAWT
                     DKIKYQVLEKYVDLKEVREQADADSIKSRTDITLEQEARNLALQATKKRMEQYVHQNT
                     DDEFFSLYLNSIVNLMDPHTDYFLPVEKRTWDENISGKFYGIGAQIGEENGYLKIAAV
                     TAGGPAWRSGEVEAGDFILKVGQGDEKPVDVAGFSTTDGVKLIRGADKSVVSLTLKKM
                     DGTIRVLKLVREELKLEETFARSNVLHIQNKKIGIINLPKFYTSFGDEKGRSCSEDVA
                     KELERLKNNQVDGIIIDLRGNGGGSLQEVVNMVGLFIPQGPVVQVKSRSGRPGIYMDR
                     DNKVIYDGPLSVMVNEFSASASEIFAAAIQDYNRGIIIGSGTYGKGTVQRPFNLNAGQ
                     SGPFDLGSVHITMQKYYRINGESVQLKGVEPDIRLRGYYENYKVKEKDQPSALPWNQT
                     SRLEYAPWAESIPEIPALRDYLQKLDSTGGQKLFEENAGWLASQNEGPRSLSENRFRE
                     QRKIIREKSAFNRDQMRLNTDQDVYGIFDGESADQPRIDRNNRLTNYYKRDRYLRLSG
                     ELMVKWLETRSIAANRTH"
     gene            complement(30233..30853)
                     /locus_tag="PKM27_08490"
     CDS             complement(30233..30853)
                     /locus_tag="PKM27_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR07339.1"
                     /translation="MVTRVIIIEDDQTIREGFTYLVNLSDGLKMVNSYASYEEAEPYL
                     VSDFPDVILLDIELPGINGLDAISRIKKKYPAAHIIILTVYENEQNIFKALSLGASGY
                     LTKNTPSSKIIESITEVLQGGGPLSANVAKMVIQSFRKNSQSPLSKRETQILEGIAEG
                     KSRTRIASELFIDKETVKTHIKNIYSKLDVNSKEEALRIARQDKLI"
     gene            complement(30916..33900)
                     /locus_tag="PKM27_08495"
     CDS             complement(30916..33900)
                     /locus_tag="PKM27_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR07340.1"
                     /translation="MFPGLIHRFFLFFPALLALAWPLISPGQKYDFVNYSIEEGLVQS
                     QVNNTAQDQYGMLWISTYGGISRFDGNTFTNFNYGNGLMHSLTSRVFVDDQNTVWIGT
                     EAGLQAYDGKGFRTIGIAPDNRRRHVNDLAQAPDGSIIALLSGWQLFKLNPGSQLLEA
                     FSDTTVSVTAVAADTSGLYAAFAKKGIYKWSGGQWQVFCPFTPADSGIVIRKMMWSHS
                     VKGLWVIGQGALFSCEQGRLVKKNTGIPSPVLCLEEDPAGDLYLGTTRGAYKLSPGQS
                     AEHIGLLQGLTDNAVFHIFRDREENLWFSTDGNGIFKFHDRSFRIYDRGSGLSGSIIM
                     GLARDHENNYWAGSLENGLSVIRDKTIRNIKLPTGKTEASKIVCVFVDAGQSVWIGTS
                     GDGLWRYRNNKFERVGWDTPRFPRHFTSINQDSRGVIWLTSPMTVSRIENGLPIPMPG
                     VYLSCFSTFEINPDTLLLGTAQGLFSINRQYELKPFEVPGTQGFVIGSINRWGDLLVF
                     GTSENGLVVWDPQTGKSYVCNEKNGLSSNMTFSVLPDGASLYVGTINGLNKVGFDKIS
                     GTFQVRPLKSANQKLGPECNQNAILKDPEGRIWAGTTKGIYIYDPKPAAPLLPPRLFL
                     KSLTIHSQLPDSTMGTDSLAAWYRIPRELRLKHRQNHLSFSLIAVHLTAPEKLRYQYF
                     LSGADTVYSAPDPVPSVIYPNLAPGDYTFRARALLDSDPPIYSSELAYPFSIKPPFYQ
                     TNWFRLLAVLFLLLTGAVIQKIRMVLRARQQRLLKEARMQEQLLIQQRTSEDLHDDLG
                     NKITRLSLLTDILQTKLGGNEEQHKLMAQIRENIQGLYMGTKDIIWALAPGNNSLMDV
                     AGRIQNFGTELFQDSRITFKSCPPDPSFEEIKPPFEYSRNLIMICKEAFGNILKHSGA
                     THASIEFSLQPSSIFDGTGLTVEIRDNGIGMPENKGMKKGYGLDNMQKRIARVGGQIQ
                     FLPNTPQGTLIRIRIKIPRTVG"
     gene            34334..35251
                     /locus_tag="PKM27_08500"
     CDS             34334..35251
                     /locus_tag="PKM27_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR07341.1"
                     /translation="MNKIFTFILAMIMMGACVSKKKHDMVTKERDSTAEEKRQLMAQL
                     KQSQDQYQDLMKKYQDEQNKLKSDMQMSESKAMELQNRIKSLEAQLEMANKTNTNLLS
                     RLEDMSIISKAGAESMQKSIDAINQQSKYIQGLNTKIQMKDSLNMQLVMNLKRSLGDI
                     NDQDVQIEVRGGVVYVSISDKLLFRSGSYDINPAGEAVLGKVAKVVNDHKEIDILVEG
                     HTDNVPIATDKISDNWDLSVLRATSVTRMLQKKFGVEPNRMTAGGRSEYAPKDSNDNR
                     EGRQANRRTEIIITPKLDQYINLLAPAEK"
     gene            35284..>35802
                     /locus_tag="PKM27_08505"
     CDS             35284..>35802
                     /locus_tag="PKM27_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07342.1"
                     /translation="MLVVFAGYQHFLFGQGPVQTGITRTFSGIPIPEVLDTLASGYGL
                     KIYYLPSENIPRDLFTGSFNNQTADEAIRTVLSKSSLQVIAVSDRIFFLTSALGREGV
                     NMNRYLSTKAKLENEYLPGGLVLGHPDSMPIQGPARILGWIRNSRDETPVEGAVVQIG
                     DADAVTDSAGKFT"
     assembly_gap    35803..35812
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35813..37994
                     /locus_tag="PKM27_08510"
     CDS             <35813..37994
                     /locus_tag="PKM27_08510"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNR07343.1"
                     /translation="TILTLPGTYSLRVISDRFSEFNQNLKVFGDDHLELNLFESTVDL
                     PELVVGANARQKLQEATSGLSQLSVKEIQRLPSLMGEADVVKALTTLPGVSTTGEVGS
                     GYHVRGGNTDQNLVLQDGVAYLNTSHVLGLFSSFNADAINNVSLYKGHIPARFGGRIS
                     SVLDIQLKDPDMKAWNFQGGLGPISSKVYLEGPVVKDKTSMLAGLRFSYSDWILRWLR
                     DPNLKNSQADFYDFSVKVNHRFKPGHVLSLSTYASKDLFNYADQFGYGWQTLSFSAQW
                     YLDLKPGMSLHASAGRSSNRNHFTETDQDKTEYRIGTGLGFNNAKARLAWQISPSQFF
                     QAGAEWTGYTPDDENYRVKPASDAATNEYYPKTRGREAAAYIQLESRISPKLNISIGY
                     RHSLYWQAGPARIRMYSPGQPRTEESVTGTKDYAKGSGIKTYQGFEPRLSLAYSTGPQ
                     SSIKLSYNKMNQYIHLISNTAVATPVDIWQLSNQHIAPQRGNNYSLGFFKNLKDHRWE
                     TSLEFYFRNSRNELEYKDFARLLRNEFLETEIFAVKGRSYGAELFIKKAVGDFRGYLS
                     YTLNRSLRKSEGLYPDEVINHGAFYASNFDKPHAINALFDWQIRKTFSWSARFVFNSG
                     RPQTAPVAEFYLGDGGHFFDYSERNEFRIPVYHRLDLSVTVTRGNIRTRKYKASWTFS
                     IYNAYNRRNAFSVFYRRNVNEPPVAYKLSVLGAILPSVTYNFRF"
     gene            37994..39076
                     /locus_tag="PKM27_08515"
     CDS             37994..39076
                     /locus_tag="PKM27_08515"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNR07344.1"
                     /translation="MNRLSIGILVVLLLAQLVSCIEEIPFASGKGFGTLVISGGINNL
                     EEDQKVFLSRTVENGGPPRPEAHALVKLVESTGRSWDFIETSEGQYTLFAGTADTEFG
                     ASYHIECTLEGGVRYQSLPEKLFPAVQPDSTGWQIGKETTRIEDGYSHSIDAFQLFIH
                     TGFAADQPETYLRWTVQSAFQFTTLPECSPFRTTVTCYFSELLNPGRFYIRSNKDQAG
                     GVLRNHPIGYESMEPFYRYSETHYFTIYQHRISQRAYEYYSRLNQVAVQNGSIFDPIP
                     ASVKGNVYRVDREDEKVLGYFQVSSIAVVRMKVVQADLAGKYNLLDKRYNLCGLYFGY
                     PDAGYFEGCCNCYSIKFPLIDKPDWW"
BASE COUNT         9679 a         9432 c        10463 g         9677 t
ORIGIN      
        1 gtaaggtgtg cccatacgat tttaaacaag ggggagtccc tcatggtcag ggtttaataa
       61 tatgatcgtt ttcttccagg ccttcaagga tttcggcata ttccaggtcc cggatcccgg
      121 tcctgacttt aacggtttcc ttttttgaac ctttcttcac gatgaccgtt ccgtccatgg
      181 agatatattc aacgggtacc gccagaatcc cctttttctg ccggatgatg atgttggctt
      241 ccagggacat gccagggtaa agatcacagc ccagaggatc gatggaggcc actaccttgg
      301 ccgtcttgtc caccggattg atcctgggat agatgttttt gaccgttcca tggaacacgg
      361 tgtcttccag ggcgaccagc tcgaagtaga ccggttgacc ctgtttcaca aggtaaatat
      421 cggactcatc caccagcatt tccacttcaa aggagtctgt cgcgcccaga tcgatgacgg
      481 catggttgct gctaaccaac tcacctgctt tgggaacgat atcataaact ttgcccgata
      541 tcgcggcgac gagcatatat tctccaagca ggcttttctg agccaggtaa ttgttgtttg
      601 cgttttgcag ttcgatccgt aactggcccc gggtctcctg gaaagcacgc gtggcgatca
      661 tgaaattgct ttgtgaagtc tggtaccgga gcctggcctg gtcaagaatt tgcaccgagc
      721 cgatgtcctg ttcgttcagg cgtttatatc tttcgtaatc cagtgaatcc tgccggtagg
      781 cggcgtaggc agaggctact ttttgttcaa gaccggccaa tcggtcagaa ccgtccgaag
      841 cgttttttct cgcctgctcc taaaggtttt tggcggtttg gagattcagc cggttggcct
      901 ggctctggat cctcagtaag gcgtctccgg ccttcacggt ttgccccacc gacacgaata
      961 tggtgtcgac gatgcctgga attttactgg tgacctcata aaagcccacc ggcagcacct
     1021 ccccggaagc gtacaccgcc tgaaccaggt cctttcgccg gataatatgt tcttccctgt
     1081 catcacagga aaacaggagg aggacaatcc agagcaatgt tgctgaatgg aaattcatac
     1141 ctctaaaatt aagacatccg cacttacaaa ctcgccaact atctgctaaa atgcgctttc
     1201 tctcccgcga cgtctttcgc ggcaggtccc tggtcctcat gcttatcgtc aaccatccgg
     1261 gtgcgaatag ggagcgatct ttgcatccca cctgcacgtg gccagtccgc tagagggctt
     1321 tgctataact aattaaatgg cattcatcca aaatttatct ggaataactt aatctgggat
     1381 tgtatatttt tagcgaacgg ttccgctggt attccattca gatgcaaaaa gcaaaaaaac
     1441 ccaggttatg aaacaggaaa gtagcttgtt attgcccgtt ttgatactat tcggcagcag
     1501 cctgtttgcc cagcaagcca gcatcgcaga aaaaattaac ggcaaaacct ttgacccttc
     1561 caccttgctc tggtatgata cccccgccca aaagtgggat gaagcgctgc ctgtgggaaa
     1621 cggccgctta ggcgccatga tattcggaaa aaacggcgaa gagcggatcc agttgaatga
     1681 agaaacctat tggtcgggtg ggccgtattc ttcagtggta aaaggcggct acaaagtgtt
     1741 gcccgaaatt caaaaattgg tgtttgaaga aaaatttctg gcggctcata acctgtttgg
     1801 caggaactta atgggctacc cggtggagca gatgaaatac cagtgcctgg gcaacctgca
     1861 tttatttttc aaaaatcagg acagcgttac ctcttataaa agatggctga acctggagga
     1921 tggcatttcg ggtgtggctt attccgccaa tggagtcaat taccagcggg aagtctttgc
     1981 gtcggcccct gaccaggtca ttgtggtccg gatcacggcc gataagcccg gccgtatttc
     2041 ttttaccgcc aatctccgtg gagaaagaaa ccaggcacat tccaactatg gtacggatta
     2101 tttccaaatg aatccctatg gcgacgatgg attggtgctg accggcaagt ccacggatta
     2161 tctgggtgtg gagggtaaaa taggatatga ggcaagaatc aaggcccagc cggaaggagg
     2221 cacgataaaa acagacggcg tggacctggt cattgaaaat gccaacgcag tgactttgta
     2281 ttttactgct gctactaatt ttgtcaacta caaagacgtc agcggcaatc cccatcagcg
     2341 ggtgaaccaa tatttccaaa gattagacgg taagtcttac ccagcaatag ccttggctgc
     2401 cgcagcggat cataaaaagt attttagccg ggtttcgctg cagttgccca agaccgcaaa
     2461 ttcttatttg cccataccgg aaagggtaat aaaaattcag accgaacccg atccttcctt
     2521 tgcggccttg agttaccagt tcggccgtta cctgatgatt gcttcatcaa ggcccggtac
     2581 ggaacccgct aacctgcagg gcatctggaa taatgatatg aatccttcct gggattccaa
     2641 atacactacc aacatcaata cggaaatgaa ctattggtcc gtggagagcg gaaacctttc
     2701 tgaatgtgct gaacccctgt ttcgcatgat cagggaattg acggatcagg gatcgcaggt
     2761 ggccaaagag cactatgggg caagaggctg ggttttccac caaaatacgg atctctggag
     2821 ggtggcggcc ccgatggatg ggcccacctg gggaacgttc acggtgggcg gcgcctggct
     2881 ttgcacgcat ttatgggagc attaccagta taccatggac aggacctttt taatggaagc
     2941 ttaccctctt atggaaggtt ctgttcagtt cttcatggat tttctggtgc cgcatcccaa
     3001 cggcaaatgg ctggtcacca atccttccac ttctcctgaa aactttccgg atggcggtgg
     3061 caacaaacct tattttgatg aggtcaccgc gggtttcagg gaaggaacca gtatttgctc
     3121 cggttcatcc atagatatgc agatcctata cgacttgttt ggttactacc tgcaagcctc
     3181 cgacgttttg agaaaggaag attcatttgt tcaaaaggta aaacgagccc gggaaaaact
     3241 tgtgccgccg caaataggga aagagggctc cttgcaggaa tgggctgacg attggaaatc
     3301 cctggaaaaa aatcaccgtc attattcaca cctgtatgga ttgtacccgg gaaaagtgtt
     3361 gtttgaaaaa agaacaccgg cattggtgga agcctataaa aaagtactgg aggaaagggg
     3421 agatgcctct accggttggt cccgtgcctg gaaaatggcg ctgtgggcca gattgaacga
     3481 tggaaaccgg gcaaacaaga tttacaaagg ctatcttaag gagcaatgct gcatttcttt
     3541 gttcgctctg tgcggcaggt ccttgcaggt ggacggaagc tttggggtga cggccgcagt
     3601 caccgaaatg ctgatgcagt cgcatgacgg gtttataaaa ttattgcccg cattgcccga
     3661 cgaatggatt gaagggagct ttagaggggt ttgtgccagg ggcgccttcg aactggaatt
     3721 cagctggcat aattcaatcc tcagccatct gaagatttta tccaaagccg gggaagtttg
     3781 cagattggaa tggaagcccg gactaaaaat caccagtaat ggaaacccgg tggtttataa
     3841 taaacattcc aatggacttg ccgaatttca aaccgtaaaa ggaactgttt atcaggttca
     3901 atagttgcca ctgaatttta aaaagggata aagatgaaac gcagtatttt atttacctca
     3961 gcacttttga tcgctgtatt gtgtggagcc ggcctttttg ctcaaaataa ggaactcacc
     4021 ctggaggata tgtatatcca taaccattac aggtcaaaag ggatcgggcc cttgcgctgg
     4081 atgaaggaca ataaaagtta ttccacactg gagtttaacg acgccttaca ggggaacgac
     4141 atcattcgat atgatgtggc tgagggatta agaaccgtcc tggttcatgc aagacagttg
     4201 ttgcctgccg gagcggccaa agccttggcc attgaagatt atgcctggtc ggaagataac
     4261 agcaaattgc tggtctttac caatacccga aaagtctggc gctaccatac ccggggagat
     4321 tattgggtac tcaacctcac cgacggcaga ctgaggcaat taggcaaagg acttggagaa
     4381 gcgaccctga tgtttgctaa attttccccg gaaggcggaa gagtggctta tgtgagcaaa
     4441 cagaatattt atgtggagga tgtcgcaacg gggaaaatca cgcccttgac ccaggatggc
     4501 ggtgggaata tcatcaacgg cacttttgac tgggtatatg aagaagaact ggatgaccgg
     4561 gatggttttc gttggagccc cgatgggacg catattgcct actggcaatc cgataccaaa
     4621 ggagtgggaa ctttttattt gatcaataat gtggattcca actattcagt acccattccc
     4681 ttgccatatc ctaaagtggg cactcccaat tctgcggtga aagtgggcgt agtgccggct
     4741 acaggaggaa aaacaaggtg gtttgcggta cccggcgatc cgcgcaataa ctacctggca
     4801 cggatggagt atatcccgga ttcggatgag gtgatgctgc agcagcttaa ccggttgcag
     4861 aataccaaca cggtttgggt gggcaatacc aaaaccatgg cgctaaaaaa tatcctgacc
     4921 gataaagacg atgcattttt agaaattcac gataatgtgg tttggctgga caaccaccga
     4981 ttttttacct ggaccagcga gaaggatgga tggctgcatt tgtataaagt gtccagagac
     5041 ggaaaacaaa tgcaatgcat caccaatggg gattttgacg tgatccaggt caactgcatt
     5101 gaccctgaag gaggttatgt ttattatacc gcttctcccg aaaactttac tcagcgttat
     5161 ctctaccgca gcaggctgga cggaagcggg gaggccgaac gggtatcgcc gatgaatatg
     5221 gccgggcagc atgcctacca gatctccgcc aacgccaagt atgccataca cagctttgaa
     5281 aacgcataca ccccccgccg gatttcactc attcatcttg ccaaccatgc agaaatgaaa
     5341 atgctggaag acaatgcgct cttaaaaagc aaaataaatg atctcggcct tcgtcccaag
     5401 gaatttttta aagtggatat cggggaggtg gtgttggatg cctgggtgat caagcccaga
     5461 tcgtttgacc cgcgaaaaaa atacccggtc ctctttcatg tatacggaga gcccgccgga
     5521 tccaccgtcc aggataactg gggcacgggc gataatctct ggcatcagta cctggccaat
     5581 gaattgggtt gtgtcgtaat cagtgtcgac aaccgcggta caaaggtgcc caggggacgt
     5641 gaattcagaa aatgcatata ccggcagatt ggcctgctcg cggctgatga tcaggccgct
     5701 gccgcaaaaa agatcatgaa tatgtattct tttattgatc ctgcacgcac cgggacctgg
     5761 ggctggagcg gtgggggtca aatgtccctg catggtatct ttcgccatgg ggatgttttc
     5821 aaaaccgcta tcgctgtttc ctttgttgcc catcagacgc tgtatgataa tatttaccag
     5881 gaacggtaca tgggactgcc ggaggataac ccggacggat accgtgaggg ttctccggta
     5941 acccatgcaa ataaattaca gggcaagttg ctccttattc atggtacggg tgatgacaat
     6001 gtacattatc agaattttga gatcatggtg aatgaactga tcaaacacaa taaactgttc
     6061 agtatgatgt cttatcccat gcgcgatcac ggcatcaacc agggaataaa caccaccctg
     6121 catatgcgca gaaccatgga acaattttgg aagaagaatt tgtaaattta aagccttaaa
     6181 aacatggtca gctatcccag catatttaat gatgtcatcg ggccggtgat gcggggccct
     6241 tccagttcgc actgcgctgc agccttgaga atggcccgcc tgtgcaggga tttgatggat
     6301 gaaaacatta aggacatcct gattgaattt gatcccaagg gttctttggc caccacgcat
     6361 aaaagccagg gttcggacat gggcttgttt ggaggattcc tgggctggga agcccatgac
     6421 gaacggctgc ccgaagcaga aaatcacctg atcaccgccg gaatccaggt gtcgatccga
     6481 atccatgaca ttggaaatca tcacccgaac ctttaccaga tcaccttatc aaacccgaaa
     6541 gaaacaagaa aattaacggc catttccacc ggcgggggaa tgattgaggt aacggccatt
     6601 gatgacctcc ctgtttccat ggccggagat tattatgaaa cattaatcta ttgcaatcag
     6661 ccggatcctg ttttatcgtt tttaaaagca tctgtggtat atgatgaact ggaagttcac
     6721 cagggcgcgt tttgtttcat tgaaataaag tcgcaggctt ttctggattc agccattaag
     6781 aaagaactgc aagaaatgga aggtgtcttg tttatcaaac aacttcatcc ggtcttgccg
     6841 gtaatggcca ggaagaactt acaggtgcct tttattacct gtaacgaaat gatgctctac
     6901 aacaaggaca ggaataaaat gctctgggag ccggcccttg attatgaaat ggccagggga
     6961 aatatttcgg cggaggaggt catggaaaag atgagaagca ttgtccgcat tatggaacaa
     7021 gcggttcatt ccggactgcg gggcactcaa tatgaagacc gaatactccc aagccaggcg
     7081 ccccattacc aaaaacaatt caaggcaggc aagttggccg gtggtgaggt gataaaccgg
     7141 atcgtcatgt atgtcagcgc catcatggaa gtaaagagtt ccatgggggt gattgttgca
     7201 gcgcctacgg ccggcagttg cggggctttg ccgggcgcgt tgatcggcat ggcccattcg
     7261 ttacatttat ccgaagacga attggtaaaa gccatgcttt cggccggttt gatcggtgtg
     7321 ttcatcgcgg cccatgcaac ctttgcagct gaagtaggcg gttgtatggc cgaaacgggt
     7381 tcgggaggcg gcatggcagc cgctgccctg gtgcaaatgc agggtggcca tctggcccaa
     7441 tccattgcag cggcctcgtt ggccttgcaa aactcacttg gaatcatttg cgatcctatt
     7501 ggcaaccggg tggaagctcc ctgtctggga agaaatgtca tggctgcatc caatgcactt
     7561 tcctgtgcca atatggcttt atcggattac gaacacctgg ttccgctcga tgaagtgatt
     7621 gataccatga aagccgtggg tgaccaaata caccatacgc ttcgttgtac caatctcggc
     7681 gggctctgtg tgacgcccgc cgccaaagcc atcgaaaaaa agctggaaca gcagcaactt
     7741 agcttttata aaagttgcta gtccctgttc ataaaaacgg ggcaccttct ccccataacg
     7801 ccggcccgct ccccaggatt ttttcatagc tgtatttatt ttctggttta tcaccagctt
     7861 gttttcaaac ttgctacctt gcaacaggtg ccttgactta tatacaacta tgcgtttcct
     7921 ttctttagac gtcttccgcg gcatgatcct cgtcctcatg ctgatcgtca ataacccggg
     7981 cgactgggga gcggtctatg cgcccctcct tcatgcggac tggcatggct gcaccctcac
     8041 cgacctggtt tttccctttt tcctgtttat tgtaggggta gcggtgcctt ttgccctggg
     8101 aaggaggaaa gaaagcggca gcagcgggat ggaactgtat aaaaagattt tttttcgggt
     8161 gctcctgatc tttggtatcg ggctggcctt aaatggtttt ccctattatc atttttccac
     8221 gatccggatc cccggcgtcc tgcagcgcat cgccctggtt tatggggtga ttgccgtgct
     8281 ctacctcaag tgttccaccc gggttttgtt ttatatcctc gtcaccctgt tgctggctta
     8341 ttggtttgtc ctgacccaga tcccggtgcc cgggtatggt tacgcaagcc ttgagccgga
     8401 aaccaacctc ggcgcctgga tcgactattc cctgttgaaa ggccatttgt ggtccggctc
     8461 gaaaacctgg gacccggaag gcctgctcag caccttgccc gccattggca cgggcctttt
     8521 aggcatcttc gccggcaatt ggctgaggag ggatggaaac ccggcggaaa aaatcaatat
     8581 cctgtttgtg acaggcggtc tttgcctttt gctgggttat ttctgggata tggttttccc
     8641 ggtcaataaa aaaatatgga ccagttcctt tgtgcttttt accggtgggc ttgccctgct
     8701 gaccctggcc acgctcacct ggctgattga cgtccgggga tataaaaagt ggacgattca
     8761 ttttgtttat ttcggcatga attcgatgat ggtttttgtg ggcagcggca tcctggcccg
     8821 cttgctagga atcatccgct ggaaccaagg agaaaaaatg gtcagcctga aagggttttt
     8881 gtattcggga ctgcaggcga cgggacttcc cacaaagatt acctcttttt tatttgcgct
     8941 tgttttcgcc ttcctttttt acttgttgct aaagttgatg taccataaaa aggtgttttg
     9001 gaagttataa tcagccgacc cgattcaggt ggaatcacca gcacacgaaa tattttccta
     9061 attttaagag cataatgaaa aaattaattc ctagcctgct tatggtattc ctgttcgcag
     9121 gaaacgccct taccgctcaa caacaaatgc ctatgctgag ccctggcgaa gcggcagccg
     9181 atctcctgaa gtccctgcgt cccgaccagc tggataaaat gtcgaggccc tgggatgaca
     9241 aagaacgggt caattgggat tttgtaccca aagaataccg ggcaggagtc acggtcggtt
     9301 cgctcagcga cggccagaag gaaaaatttt accgtctggt cctggctacc ggtggggaaa
     9361 agaccctgga ccgggtgaaa tccatttctt tcctggaaag catattgcga ggcgtggagg
     9421 ggcggaaaga aggcgatgca taccgggatc ccgggaaata ttttatccaa ttgttcggcg
     9481 cgacccacct ggacaaaacc tggggctgga aaatcgaagg tcatcacctt tgttttaatt
     9541 atgtgcttga aaaaaataag gtgattgccg cttcaccttc cgtgctgggc gccaacccgg
     9601 ccatcgtcct ggaaggcgaa aacaagggtt accaactgat gaagccggaa atccagctcg
     9661 ccacgtccat cctctcctcc cttaacaccg gccagaaaac caaggcgatt gtttccagcc
     9721 aggcctccaa agaaatcctc acctaccagc aaaggaaagc cagcatcgac aaagccgggg
     9781 gcgtctcatt tacggaattg agcccggccc agcaaaccaa actgaaagaa ctcgtggaat
     9841 tatacatcca ccgcgcttcc agattttttg taaaggacat gcttgcgcgg gtggaacagg
     9901 ctggctggaa caaggttttc tttgtctggg agggttcaga agacgtgagt cccgggcata
     9961 cccattatta ccgcatccag ggcccggaat ttattattga gtacgacaat taccaaaaca
    10021 acggcaatca cgtccacagc atcttccggg atgtaaggaa tgatttcgga gatttgctgg
    10081 cggcgcatta taaggaagag catccctgag tcgccctcac cacctccctc ttccccgccg
    10141 gccccctgag tttttccact ctccaagccg ccttttccag attccggcgg accgaccctt
    10201 tggaactgta ggtggtcaaa accgatccgg ggtgggcagc aagggcgagt ttttcaaaca
    10261 ccgtatagtc ccaaagatcc ggttgagaat ccggatcgaa ggcatcgaag aaaaccacat
    10321 caaagacttc agtcaagtct aattgcctta agtcctgttt gtatttgacc aaagtaaaaa
    10381 agggatgaat ccgggcaggc ttgtcccatg ctgcttcatg cagtgggtcc agaagggcag
    10441 cctcgccgcg gtagagctgt gtgtaaattt cagcagggag cgggtataat tcgcaagtat
    10501 ggtattcgat accgataccc gttttttggg catattgggc agccaggagg gcgttcaggc
    10561 ctgtcccgaa gcccatttcg aatattttta cggatgaaag gccagggttt tcactggtaa
    10621 aaaaatgtaa tccgttgacc aggtatacct gctccgattc cgtccgggct ccaaaacggc
    10681 tgtggtaact gactcccaaa ggatgctgca tgaccgtctc agacccgtcg tccgtacgaa
    10741 agacttcgaa gagcatgaat tagtttgcaa gttagcaagt tagcaagtta gcacagtcag
    10801 ggttcataac tcatagctga cggatcttta tctttggccc ggatgaaatc ggtgcataaa
    10861 gtccagatcc agctcagtac accggaccct gcaggggagg cagtctgcat ttccggttcc
    10921 tttaaccgct gggaggaaaa cgaatacaaa atgaaggaga tccggcccgg agaataccag
    10981 cttgatctcg aattgaacgt accggaggac catccggtcg aatacaaatt tattcgcagc
    11041 cggtgggcga atgtcgaact ggatgaattc ggcagtttta cggaaaacag gatcctgacg
    11101 gatcccgatg aaccggtcag ggcctgggtt ccccgttggc gaaatgaagg cagggtctgt
    11161 gacatctccc tgattccgca agttgaaatt catcacgata tggtgatcac cggtacacgt
    11221 aaaaagcgca gggtttcggt gctcttgcct tacgactacg catataccga taagagatac
    11281 ccggtgctgt acctgatgga cggtcaaaac ctctttgaag accacgcgcc tttcggatcc
    11341 tgggaaatcc ataaaaagct ggccgtgatg gctgagaaaa acatgcatga agtgatcgtg
    11401 gtgtgcatcg atcacgcggg actcaaacgg attgaagaat acacctatat ccggcacaac
    11461 agcactgaaa agggcagggg acatctgttt ctcgattggg tggtgggcag gctcaagcca
    11521 tttattgaca aacactaccg gaccaagccg gagcccacca ataccggcat tgggggcagt
    11581 tcgatggggg gcctcatcgc cctgatggca gggatcaacc agccccggta tttcggcagg
    11641 ctcatgttgt tcagcccctc cctttggaaa atcctggacc tgatttacga ccggctgatc
    11701 tacctccccc tgatcagcca cgaccagttt atttatctct atgccggcgg aaaggaagct
    11761 tcgaatatga aggggttcgc ccttgatttt tttgaccagt tgatccgcac ggacccctcc
    11821 aacgaccggg tccatctgtc gatccatgaa gccggggaac atagtgaaaa gtattggggc
    11881 aaggaatttc catatgccct gaagtattta tttttctgat ctttacagca atgagaccaa
    11941 taccgattta ttccccggaa gatatgcggc aggcggatta tatcctgatc cctgtattca
    12001 aaaaagacca caaaaaatta ttgaaatacc tggccgcctg cgaaggccag ctgccttccc
    12061 cctggtcaaa acaagcccta ctgttggcgg aaaaccatga accggactac aaaaagttta
    12121 tcgagcagtg ggtggggaaa caaaaactga ttttgatggg gctcggggat gaacgggctt
    12181 atcagaaaat tgcgggagta tgccgcctgg cggtgcataa acaggaagaa aagccgtccg
    12241 gaggaaaaat cgtcctggtc ctgccggact atctcgatgg aaattccata tcggccctgg
    12301 ccaatggcct cgctctttcg gcgatccggc tggattatta taaatcagaa aaaaaaggta
    12361 aaccggccga gcttgctttt ttcaccggta aaaaatcgac tctgaaaagc attgaagccg
    12421 ggatccgcat cgcctctacc caggaagaga tttgccacct ggtcaatctg cctccgaacg
    12481 agatcaaccc gcaatacctg gccggctggg cccgaagagc atttcacggg aggccggtga
    12541 aggtggagat cctggaagaa gaggccctgg cccagcacaa gatgggcgcc ctgcttgccg
    12601 tcggcatggg cagtgagacg cccccgcgcc tgctggtgtt ggactatcaa ccctccaccc
    12661 gggctaaaaa attaaaacat atcggcctgg tgggcaaggg ggtcaccttt gatacgggag
    12721 ggatcagcct caaggatccg gccaacatgc acctgatgaa aagcgatatg ggaggggcag
    12781 ccgcggtgct gggcgccgtg gacctggctg aaaaatccgg cctggccatc cgactgaccg
    12841 ccgtgatccc gcttgcggag aatgcgatcg ggccggcatc ctaccggccg ggggatgtga
    12901 tccattccta cagcggcaag agcattgaaa tcatcgatac cgatgccgaa ggaaggctga
    12961 tcctggccga cgccctggcc tacctggtaa aaaaatacaa gccggaaatc atggttgacc
    13021 tggctaccct caccgggagt tgcattatga ccctgggaaa caaagctgcc ggcctgttca
    13081 ccaataatga aagactggcg cagggaatta ccgcagccgg ggaggcaacc ggtgaaaggg
    13141 tctggcgcct gccgctttgg aaggaatttg aagaagaaat gcattccgac attgcggaca
    13201 taaaaaacct ggccaccaaa ccggttgccg gggcaagcac agcagccaaa ttcctggaag
    13261 ccttcacttc gggccatccg gcctgggccc acctggacat tgccggcgtg gccatggtgg
    13321 attcggattt cagcaaaatg cgcagcgcta ccgcgtatgg agttcatttg ctggtgaaat
    13381 ggatggaaga aatgatcaag ccctgaacat ggaaagcccg cttacctttt tatgcattgc
    13441 ttcttatttc aagggcaatc ggtttatgca ggccctcaaa caatgcgggg ccagggtgtt
    13501 tctcctgact tccaaaaaac acgaagacaa agcctggccg cgggaaagcc tcgatgaaat
    13561 tttttatatg gagtccgatg agcgcaacca ctggatcatg gaagacgcgg tcaaggggct
    13621 tgctttcctc atgcggcggg aaaaaatcga tcgcatcgtc gccctggatg attttgacgt
    13681 ggaaaaagcg gctttgttga gggaagaatt ccggataccc ggtatggggc agacgaccgc
    13741 ccggtatttc agggacaaac tggccatgag gctcagggcg caagccacgg gagtagccgt
    13801 gccggctttc acccccatat tcaacgatga gatggtcaac ggctttatcc gggatacgcc
    13861 cggcccctgg atgctcaagc cccggggaga ggcatcggct accggcataa aaaaaataga
    13921 aacggccgaa caggtctgga agcaaatcca ggagcttggt gaagaacgtc accagtacct
    13981 gctggaacgc ttcttaaaag ggcaggttta ccacatggat tccattgtgc aggatggcat
    14041 ggtgcttttt tgccgctgca gccaatacct ggcccctccc ttcgaggtag ctcacggggg
    14101 aggcattttc aggtcggtca ccctggaacc ggaagatgaa gacaaccggg ccttgctccg
    14161 cctcaacgag gatatcctcg gcggctttaa cctccggtcg agcgcagccc ataccgaagc
    14221 cctgaagagt gaagaggacg gtaagtttta tttcatcgag acttccgcaa gggtaggggg
    14281 cgcccacctc gccgaaatgg tggaatttgc caccggcatc aatctctggg aagaatgggc
    14341 aaaggtggaa tacacggctg ccttatatca accgcatctc ttaaaatcct gggaccccgg
    14401 acatgcgggg atcctgatct ccctggcccg ccacaaaagt caggattaca gcaggatctc
    14461 tgacccggag atcgtatgga cgatgaccga catggaccat catttcggct gcattgtcaa
    14521 atcccacgac cgccggcgca tcattgaatt gctggatcaa tacaccgctt tggtccaggc
    14581 agaattccac gcaaccgctc ctgcaccgga taagccgatc cattgagttt tagtcacatc
    14641 ctgcgacctg tccttgctgc aacagggttt tgtcacgggt tcaatcacgc gttcaaatta
    14701 aataagtaag cacccggata aagcctgatg gggccgtcat gattaatcaa aatctatagg
    14761 attagaggct tatttataat ccggaactta tgccttaaat cgctgatatc aatcaagtta
    14821 aatagtgatg attgtcatca tttattgatg ttatccctat tacattgaca ggttgaggtt
    14881 gaatatttta ttcaattttt ctaaagcgtt tttttatgaa agcaattttt attctatcgg
    14941 tgtttttgtt atatactgca aactccgcgg gatcaggaat ttctggcttt gagcccggtc
    15001 attcaaattt tgcaatggac ctgaaggcag ccggacccgg gagcgggggt gcttatagga
    15061 tgaatgaaat gatgatccgg gacctcatgg catcggatcg ggtatcccgg caggacccta
    15121 ccggagatct catggctttg gtgggggatg ctgcgcaatg gatcaggtta aaaggggaaa
    15181 tggcctttga tgattttcgt gtttccggca gccgttggcg ccaggaagag acttatattt
    15241 ttgtgctgga tccctctggg aatatgctgg tccacaacga tcctgaactg gaaggcaaga
    15301 accagctggc cctgaaggac atcaacggga aacatatcat tcgcgggctc ctcgatgcgg
    15361 tcaatgccat ccccgataaa ccggaaggat ggtatcatta ccaatggcct gttccgggcg
    15421 ggctgctgcc ccgatggaaa agcagctatg tacagaaagt gaaagcgcct tcaggcaaga
    15481 gttatatcgt aggaagcggc acctacaatg accgcatgga gcgggaattt gtggtggatc
    15541 tcgtaaaaag ggcagtcaac gaaataaata agcagggtaa ggcggccttt aaattgttcc
    15601 acgaccccag cggccccttc ctcgccaagg atgcttatat tttcgtcatt accatggagg
    15661 ggatcgagat cgtcaatccg gcctttccga gcattgaggg acgcaataac ctggatgtaa
    15721 gggatcctca gggcaagtat ttgactcggg aaatcattca ggtcgcacag tccaggggta
    15781 ccggttgggt tgactacatg tggcccaaac cgggagaaag tgtctccact caaaagtccg
    15841 cctatgtggc gagggccatc ctggatggaa aaccggttgc ggtgggctgt ggggtttacc
    15901 tgggtgagac tcccaaaatc atcgcccccg caaataaaat gacagcaacg gaactgatgg
    15961 ccctggttcg cgaaggcgca gccgttttgg aagagaaagg ggagaaggcc tatcccgaat
    16021 tccggacgaa gggctcccgg tggtaccgcg acgatactta tttctttgtg aacacgatgg
    16081 aagggatccg gcgttttcat gccacagaac cctccagcga gggccaaaat gtggtcggcg
    16141 ataaggatgt gttgggaagg ccgatcggaa agatgagtct cgaggcggcc ggcagtgcct
    16201 ccggtgaagg atggatccat tatatgtggc cggaacccgg ggatatattc cctaaatgga
    16261 aatccacttt tgtaaagcgg gtgacttttc catcagggaa gcaatacctt atcgggagcg
    16321 ccatctatca attacaaatg gacaaggcct ttattgaaga tatcgtagac cgggctgcag
    16381 tattggtggc cgagcgcggt aaaggggctt ttgacctcct gcgggataag aaggggcctt
    16441 tcatgtttat ggatacttac gtatttgtaa acaatacggt cggggtggaa ctggtcaatg
    16501 gagcccagcc cagcctggaa ggcaaaaatg tcatcaacga gagggacctg aacggcaaac
    16561 gggcagtgta cgattatatt gaagctgcct tgaaaaacgg cagtgcctgg attgattatt
    16621 actggtaccg gccggggggc aatgaaccgg cccgcaagca cacttatgtg cgcaaggtgc
    16681 agcacggcaa tgaaacctac atcgtcgggt ccgggtttta cgaagtggat gcaggacaag
    16741 aaaccggaga agctcgcaaa cattcctgga atgcgattga aaaagaggag atgagcaaat
    16801 ccttgtatcg gcaggccatc agcggggaaa aaggtaccct ttcacaattt tcagcaaaag
    16861 ggggcgcccg gatagccctc cattcccatc ccagtgaaga gtattttgtg gttacgtccg
    16921 ggtccgtaaa atacagtgtg gaaggcaggg aatatatcat catgccggga gaaatgatga
    16981 tcactccttc ccaggtgcct catgaaatag aggtattgga agaaacggtg tttgtggttt
    17041 tcttcactcc ggccagggag gattggctgc gcggggcaga ccattatctt cgatatcaag
    17101 aaaataaatg aaaggacaga tccggttttg aataaggcga gtaaatagag aaaaaggact
    17161 taacggatta aaacaataat ggagtttgta atataaaata tgaggtttag aagtatataa
    17221 gatttttaaa agatccgtct gttcatacga tctattggtg aatcaatgga gagtatatta
    17281 ttcaaaaccg gagactgttt atatagatat ttttttaccc gtctgtcgat ttgtgtaata
    17341 atttaagcta tgactttaat caaataaacc ggtgatggta atcatcaaag ccggtggctt
    17401 aaagacttag tttaggggat tcaattattt attatgaaaa aaggcttatt cacccggatt
    17461 gccttttcag cgggtctgat ttgtgcgact ccggcctttg ttttttctta tcctgatttt
    17521 tttaaaccaa ataaaccaaa cggtatgagt ataatagaac aagcgacagt tttagaaaaa
    17581 gaattcaccg ggacggggat gatcaatgcg ccttcgaatt ccggttttac ggtggcgaaa
    17641 gacagcaccc cggaaatcat ttccctggta agtgatgctg ccgacctgat ccgtcttaaa
    17701 ggtgaggccg cctttgatga attccgccgt ccaggcagcc ggtggcgcat ggaagacact
    17761 tatatttttg ttctggatcc ttctggaaac atgctggtgc atgccgatcc ccagatggaa
    17821 ggcaggaacc agttggccct cgaagacatc aacggcaagc cgatcatccg cggtttgctt
    17881 ggggcggtaa ccaatctgtc ggataaacgc gagggatggt atcattatca atggcccgtt
    17941 ccgggaggtc tgttgccccg ctggaaaagc agttatgttc gcctggtgca cagtccgacg
    18001 ggaaaatcat ttattgtggg cagcggggtc tataatgacc ggatggagcg ggaattcgtg
    18061 gtagatatgg taagggatgc cgtggggcaa atcgaaaaat ttgaaacggc ttctttccgg
    18121 ttgttccatg atccaaaggg tccgttcatg gcaaaagatg cttatatttt tgtgatcaat
    18181 gaacagggcg tggaccttgt aaacccagga tttccaaacc tggaaggacg caatatcatg
    18241 gatctcaaag atacccgagg taagtacctg atccgtgaaa tgttccgctt ggttgaagcc
    18301 gagggtgccg gttgggtcga ttacatgtgg cccaaaccgg gagaaagcgt atccactcag
    18361 aaatccacct atgtcacgcg ggcgaggatg gggaaccaat ccgtattggt cggttgcggg
    18421 gtttatctcg ccgatgcccc caaggaacct gccctgacca aaaaaatgga tgctgctgaa
    18481 ttgatggccc tggtgcgcga ggcagcggtg atcttcgaaa aaaatgggga aaaagcttac
    18541 ccacagttcc gggagaaagg gaccaaatgg tacaaagagg atacctactt ctttgtctgg
    18601 aatatggaag ggatccgggt ctttcatgcc gctgaaccag ccggcgaagg acaggatgtg
    18661 agcaagctga aggatattca cgaccggccg attggatata tgttcctgga tgccgcttcg
    18721 acgggggcaa aggaaggatg ggtgcactat atgtatccgg aaccgggaga cattttcccc
    18781 acctggaaat ccaccttcgt caaaggagtg acttttccgg acgggaaacc ttatctcatt
    18841 ggttgcggaa tataccagat ggagatcaac aaatccttcg tggaggatgt ggtggaccgt
    18901 gcagctaaac tgatagccaa gcagggcaaa caagccttcc ctcaactccg cgataaaaca
    18961 ggcccttttg tattcatgga cacttatgta tttgtgcatg accttcaggg caccgaactg
    19021 gtaaatgctg cccaacccag cctggagggc agaaacctgc ttgatctgaa agacctgagc
    19081 ggcaaagagg taatgaaaga acagctcgag gccgtcagga atgcagagag tgcctggctg
    19141 gattgttact ggtttaaacc cggggataac ctgccggccc tcaaaaaaac ctatttgcgc
    19201 aaagtccgct ttggccccga aacctatgtt gtgggctccg gattgtacct gtcaggcgat
    19261 gaagggaaac cggcaggcat ccggaaagta tcctggaaag acctagaaac cgtggatttc
    19321 agtgaacgac tgtcccgcca gatgatcagc ggtgaaaaag gcacgctggc caggtttaat
    19381 gccaaaaaag gagttggggt cgaccgccat cagcacgata atgaagaata tgtatgcgtc
    19441 ttgtcggggg tgatgaaatt catttttgat gaccgggaag tgttgctgaa aaaaggagat
    19501 gtcctgatca ttccggcaaa cgtcgctcat gatattgaag tcaccgaaga tgcagatttt
    19561 ttcgattttt tcattcccct gcggtcggat tgggtgagag gggaggatca atacctgcgt
    19621 aaagaaaact gaggcatgaa cgggtaatcc tgtagattac tcctttcatt aaagcggcta
    19681 acaccataag atgtcaatcc tcttcagggg gattgacatc tttcttaagg aaaaagttaa
    19741 ttccggtctg gatggggcac aagaggccct gttgcccgtt atgaacctaa cgatggtggt
    19801 catgtgccca cggtatgaat atggtttggg tttaaccctg tttttattgt tcaccggctg
    19861 tgtggaggaa gcacggtgga accaggatat ttttgtgctt tatcatatcc acaagataaa
    19921 atccctttcc atgatccgtc ttccggagga ttaccggatc aaatctttcg atgtcctgga
    19981 tgaccacagc tcctaccagg taaagttccg cctggattat catgaaaatc agatccggga
    20041 catgtccgac cggttcaggc aaatcaggag ggagtccagc ggcccgggtt ggggaaaata
    20101 tccggcggga tgggagtttt ttcatcagcc cctgctcgat gagcagttta tcctgcggat
    20161 cgataccctg gccggcgagg tggaatattc gtatatgctt ttgtattaga ggaagggaaa
    20221 aaagggagga agggagaaag ggagaaaggg atgtcccttc gctaacgcta tgggacatcc
    20281 gcaggaactt attgcttcag tatttttctc gaagtatagt cgtttccgtt ttcaattttt
    20341 agcatataga gtccggggat ccaggcatgg gtattgacct tgatcatatc ttttaaatat
    20401 ccggagaaaa caatctggcc caggatgttg tggatttcaa attttaattt ttcggggttt
    20461 ccatccatgg ataaagtcag ctcattgaaa accgggttgg gataaacccg gagtccctgc
    20521 caggcggagg catcgtcatg gcccgtgctg gccagttgaa tattccagtt gagtacgtgg
    20581 gtattcaggg tgggatggtt atcggcgcgg atgagggggg tggtgtccag gacttcaaag
    20641 gtcaaggtgt tgtttcccgg aagtaattga tttggccgca ggaggatcga atcccggttc
    20701 ataaagatca gttggccatt caagatccat ttggtcctca gcgtattcgg attgggtttt
    20761 tgcaaatcga ctttaaaagg aatggaatcg gccatcaggg tcacggatgg atctgcagga
    20821 aagcgggaca ggaccggtgt cccgaaatgt tgcatgatcc ggagcacaat cgcttccttg
    20881 catacggggc aaaattcact gttcaggaag cgcatttcgc aggattcatg cggtcgaaac
    20941 cagttttcct ggccaccaga ggcgccgtga gggtatactc caaccccgtt aaaaccaatc
    21001 cagtttttcc atcttacttt ggccgggtct ttttcacggg acatattggc tctttctccg
    21061 gcagagaatt cattcaccca gtattcgtcg gcgaggccgg caaaggaatg cccgatttca
    21121 tggaccatga tttcaaagga agaagtattg atcgaagcgg tagccgcggt gcttcctccg
    21181 gatcccccgt aaaaagtact gttgaccagc atcaatggct gatcgtaaag cggaaggttg
    21241 tcgatgatga cattgatgac cgcccccgga ttggtaggga ccagcaaacg gtggatggaa
    21301 ctgtaatcaa aagtggaatt gaaatagttt tccgtaacca acagggggtg ctcggctgcc
    21361 ggtacacaat cgactgccgt acggggatgg tccgctccgc tttgagctga aggaactttt
    21421 atcgcgtaga cattaaaata atcggcatat tccttaaagg gcgaaaattt gaacaagtag
    21481 tctgcggtat tcccgacatg ttcctggtat ttgttgagat cgccggcctg atacccatcg
    21541 cccagaaaga ccaggttgat gaatttcttg agatctccgt tccaccgtat gctgtcttta
    21601 ggaaaggttt gtgcctgtaa ggcgggggca aaaatcaaaa agagaagacc agcgccccat
    21661 cgataaaagg ttaattgtgg tttcatagct tactgggtcc ggattttaat tcccgggtaa
    21721 tttcagggta ttcaaggttt ttaattgttt gtttttttcc actttgtgga cctggagata
    21781 ggtcatcctg gaggtgtagg caaaacggag caggacttcg ttttcctgca attccacttg
    21841 ttttgatcca atggctcctt cctctccggg aaattcatac ctggggtgga ggggctgtga
    21901 gacgatgagg gtatccaggt tttttttatc ccggtcctgg ataaaacaga ggaaatcatt
    21961 ttcccggtaa atcaccggac ccggttcgtt gcccataaag ggggcgtcga tgatccggga
    22021 attgggaatc gtaacctggt aatcgtttcc ttttttttcg atgcgcaggg aaagcaggag
    22081 ggtccttggc tttccggatt cctgttcagg gggggcctgt gtttttttgg agcaggcaag
    22141 gaacggcaag gaaatcacca aggccagaaa ggtcaggact ttcattttgc aatggtaaga
    22201 cggacaaaaa ggatcgtggg ttggtgaatg gattgacctt cttgtttgga caggctccag
    22261 ggtcgggggc accggggcat gcttcatttc actgagtttt cccatttttg acaacggtta
    22321 gtaaataaaa ataaaaactt atggcaaaat tcttcttctt ggcgatgatg ggctttactc
    22381 tttggactgc agggctccgg gcacaatccg cccaaagcct gttgaatgag cccctggatc
    22441 tcagccgcga tttcagggat tattccaata cctatttcct ggcagacagc ctggcaagct
    22501 ttgatccggc aaaggcatcc ggacaggtat tgtggagaag ggctgaatat tttccggccc
    22561 atgccttcaa ttatacccag catgggatcc gaaggacggt ccagaatgaa tttcctgccc
    22621 gggaatatgc ggccgacccg gtctggcctt tccagttgga attcataacg ccgaagacgg
    22681 tccggataag gatgaatacc ggagtgagcc gggtcgaaac ggataccctg atgatccccg
    22741 gaggccgggt tgcttcagac aaaagctgga tctacacccg gatcgagggc ggccatcagt
    22801 ataaaagcca gcagggcatg gtgcgaatct atgaacaacc ctggggagtg gaattttttg
    22861 accacgaggg cagaccgctc actaaaaccc ggcattcctc cgacaacaag ggcttttgtc
    22921 aaaccatgcc tttttgttat gtgcgccggg ctgcggatta cagcaggagc gtcggcgcgg
    22981 tctttaccct ttctcccgat gaaaaaattt tcggatgcgg ggaatccttt accaaactca
    23041 acaaatacgg ccagaaagtg aatctctaca cctgtgatcc aaacggagtg gaaacgccgg
    23101 gcatgtacaa acccgttcct ttttttatga gcagcagggg ttacggcatg tttatgcata
    23161 cctccacccc gatcacctgt gattttggaa acagctatgg ggcttccaat gccttgtgga
    23221 tcggcgatga ggccctggac ctttttgtat ttatgggaaa acccagggac atcctggatg
    23281 agtacacccg gttgaccggc aaagcgccca tgcccccgct ttggtctttt gggctttgga
    23341 tgagccggat cacctatttt tccgaagccg acggaaggaa tgtggcggcc aaactgcgcg
    23401 ccaaccgcat acccaccgac gtccttcatt ttgatacggg ctggttcgaa acggactggc
    23461 gttgcgatta tgaattttcc aaaaacaggt ttccggatgc ccggggcatg atcgccgacc
    23521 tggacaaaga cggttttaaa acctgcctgt ggcaactccc atattttgtt ccgaaaaaca
    23581 aattgttccc ggagattgtg gaccagggac ttcatgtaaa aaacgcgaaa ggcaacattc
    23641 cttttgaaga tgccgtactg gattttacca atccccgcac tttggactgg tacagggaaa
    23701 aaatcggcgg cctgctaagg atgggcgtag cagcgatcaa agtggatttt ggcgaagcgg
    23761 ctccctttga cggcatttat tccaatggac gcacgggatt ctatgagcac aacctctatc
    23821 ccttgcgtta caaccaggtg gtttccgacc ttacccagca actgcgcaat gaaaggatca
    23881 tctgggcgcg gagcacctgg gccggcagcc agaggtatcc cctgcattgg ggaggggatg
    23941 ccgaaacttc ggatatgggc atggaggccc agttgagggg cgggctttcc ctgggccttt
    24001 cgggcttcac tttctggagc cacgacatcg ggggctttac gcgcaggaca ccggaaaacc
    24061 tttaccggcg gtggttgcct ttcggggctt tgtcctccca tacccgctgc catggacagc
    24121 ctcccaagga accctgggat tacggggagg attttcaaaa ttatttccgc aaggtcatcg
    24181 aaataaaata cagcctgatg ccttatatct atacccagtc gctgatgtca tcccaagccg
    24241 gtttacccat gacccgggcg ctgttgttgg aatttccgga agaccgcggg gcctgggaaa
    24301 ttgaaaatca gtatttgctg ggatccgaca tcctggtagc acctttgttt gaagagggcc
    24361 ggctttcgag ggaggtatac ctgccggcgg gtaagtggat cgatatgcag gatggcaggt
    24421 cttattcggg aggctggcac cggatgtccg caggggcggt gcaagccatt atcctgatcc
    24481 gggacggtac ggtcctgccc cggctcaaag ctgcccagtc cacccgggat ctggactgga
    24541 gccaggttga tctggttgtt ttcagtacca gttcggaggc gaagggtatg ctggcggttc
    24601 ccggtgaaac ggaaatccgg aatctcaaac tgataaaaaa gaaaaaggga ttcgaactgg
    24661 cacaggaagg attgccggtc aatgtaaatt ttaaaattgt gccgtttgag caggctttga
    24721 taaaatgagt tttaccgggt cggagctaag ctcggaagat gttgagacga agcttggctt
    24781 cggcttggta gcgggtcgga gctaagctcc tcccccaaca catatgttca gacgaagctt
    24841 ggtttcggct tggtacctaa gaaaaatgat ttacagtttt acggggggtc ggagctaagc
    24901 tcctccccaa cagtgtttag atatgccaaa ttttaaaaat ttggcatatc tataagctca
    24961 actaaaccag gttgtccagg tccaccggaa gtttataaat gcgtttttgc gtcaggttaa
    25021 aaatggcgtt gccaagggct ggtgcgatgg gaggcaagcc gggttcgccg actcccccgg
    25081 gattttccag gttgtccatg atatggactt ccacttcagg catttcagac atgcgcatga
    25141 cctggaattg gtggtaattg gtttgatcgc atttgccttc ggaaaaagtg atgcctccct
    25201 tgatggcgga acttaaaccc atggcgatgt ttccttcggt ctgggctttg acattgtccg
    25261 ggttgacgta ctgcccgcaa tcgatgatcg aagtgacttt cacaatccgc gcccctttgt
    25321 ccttgcgggc aatttccacc atggccgcgc tcatggactc aaaagaccgg aacatggcga
    25381 tgccgcgagc atggcctgtg ggcagggcag gggaggattt gatgatctcc tccagttttt
    25441 ccaaaatggc gaggtcgcgt tttgaattgc gcaacagttg tttgcgtgcg gccagggggt
    25501 ccaatccggc tttatgggcc atctcatcga taaaacattc ctggccccag gcaaaattgg
    25561 aggcatatac cgaacgccac caaaggatcg gaatcggggt ttttaccctg ctgtaggaaa
    25621 ggtggtgtac cgggaatccg tatttgtgat tttccttgct gagttcaccc gccagccaat
    25681 cgtcagcctg gtaatccgcc agcccgttcc aatgctggcc ctggatggat tctccgatag
    25741 cctgatggct gagggcggac agccggttgt tgccgatcac gccccgcatc cggctcagca
    25801 tggccgggcg gaaggggccc tgcatcatat cttcttcccg ggaccagatg agtttgaccg
    25861 gcgctttgac ctgcctcgac aggtcgatcg cctctttcat aaaatccatg aaggctttgc
    25921 gtccaaagga acctcctaac aattggggat ggatttttat tttatctgtc gcgatcccga
    25981 ggtaatccgc cgtctctccc aatgccccgt ccgggccctg tatgggagcc cagacttcca
    26041 ccgatccgtc ctctttaaca tgggcggtcg cgttgaccgg ttctatggcg gcatgagcca
    26101 ggaagggggt ttcatagatg gattccagtt ttttctcgga agccgcaaag aaagcatcaa
    26161 aatcgccgtc ttcttccgca cgcaccccgg gctttgcggc agcggcgtaa gcgtctttaa
    26221 aatattgacc ggtattcagg gtttgcacca ggtcgccatc gtcccattgg gcggtcacca
    26281 gtttgctggc tttgagcgcg gcccagtaat tttcggcgat caccgctatg gcttccgact
    26341 tgccgcccgg gtaggaatgt tccgttttga tcacctgctt cactcctgcc acggccatgg
    26401 cttgcgtggc gtcataagag accagtttgc cttgcacgga gggagcatgg atcaccgtgg
    26461 catagagcat gccgggaacc acgacgtcga tgccgaagac cgctgtcccg ttgactttgg
    26521 acgggatgtc gcttctttta tggtatttgc cgaggatctt aaaatcctta ggatccttca
    26581 gtttcggatt tttgggaaca tccagtttgg cggcatcgac ggcgagttcc ccataactga
    26641 attttttatc cgttccggtc aggaataccc cgccatcccg ggcctggcaa ctctccggac
    26701 tggtattcca tcggccggcg gctgtcctga ccagcatctc tttggctgcc gcacctgctt
    26761 tcctcaggtc aaaccaaagt cctctcaccg tactgctgcc tcccgagccc tgggacccat
    26821 atttggcctg accatcggaa tgcacgatct ggatctgatc catacgcact tcaagttctt
    26881 cggcaatgag cgaagtaact gcctggattg aaccctggcc catgtccggg cggctgttta
    26941 cgagggtgat gacccccttg gcgtcgatga ggacaaaggg attgagttcg gtgcttatat
    27001 tgttttcaaa gagtttgacc agggcgtggc ccccggcatg tgcccgcacg ccgatcatca
    27061 ggccggccgt gcccagcaaa ctttgtttta gaaatcttct tctttccgtt gaaaggcttg
    27121 aaatggacat atcaggatgg aattgaattc atgaagaata aggatcagaa cccatcaatg
    27181 ggtccccatg gtttttggtt ttgcagagcc ggaggcgcgg tggatggctt ttcggatccg
    27241 gtcgtaggtg ccgcagcggc agagatttcc attcatatag gtgtcaatct ggtcatcgga
    27301 aggttccgga tgctgggcca gcaaagcgac ggcggacatg atctggccgc tctggcagta
    27361 accgcattgg ggtacttgct cctccaccca ggcttcctgc acggcatgca gcagttgcgg
    27421 gctgccaata ccttcaatgg tggtgatttt ttgctttttc tttatcacgc ccaaaggcag
    27481 ggaacaactt ctgaccggct gtccgtccag gtggacggta caggcaccgc attgggcaat
    27541 gccgcagcca tatttggtcc cctttaaacc gacaaggtcc cggatagccc agagcagggg
    27601 catgtcttct tcagcctcta ttttatagcg ctggtcattg atggtaaggt tgaaaatagc
    27661 cataaggtga attgaaaagt aaaaatagtc ccaattgtta agataaaggc agaggtaaag
    27721 cttaacgaaa gtttaaaata tcaggcgagc tgaggcgggt ttatgcaacc ttatcctgcg
    27781 tgagcgagaa cctgataacc ggaaaggccc ccggctttag tgggtccggt tggctgctat
    27841 cgaacgggtt tcaagccatt tgaccatcag ttcgccactg aggcggaggt aacggtcccg
    27901 tttataataa ttggtcagcc ggttgttgcg gtcgatccgg ggttggtcag cggactcccc
    27961 gtcaaatatc ccgtagacat cctggtcggt attcaatctc atctggtcgc ggttgaaagc
    28021 ggacttttca cggattattt tccgctgctc cctgaaccgg ttttcggaaa gagaccgggg
    28081 gccttcgttt tgtgaagcca gccacccggc gttttcttca aacagcttct ggccgccggt
    28141 gctgtccagt ttctgcaaat agtcccgtaa tgcggggatt tccggaatgg attctgccca
    28201 gggggcatat tcaagccggc tggtctggtt ccaggggagg gcgctgggct ggtccttttc
    28261 cttgacctta taattttcgt aatagcctct tagcctgata tcgggttcta cccctttgag
    28321 ttggacggat tcgccattga tccggtaata tttttgcatg gtgatgtgta cgctgccaag
    28381 gtcgaagggg cccgactgcc cggcattgag gttgaaaggc ctttgcaccg tgcctttgcc
    28441 ataggtcccg gaaccaataa tgatccctct gttataatcc tggatggccg cggcaaaaat
    28501 ttctgacgcg gaggcgctga attcattgac catgacggat aaggggccgt cgtaaatgac
    28561 cttgttgtcc cggtccatat atatcccggg ccttcccgaa cggcttttta cctgaacgac
    28621 aggtccctgg ggaataaaca gccctaccat gttgaccacc tcctggaggc tgccgccccc
    28681 gttgccgcga aggtcgatga tgatgccatc gacctggttg tttttaaggc gttccaattc
    28741 tttagccacg tcttcagagc aggagcggcc tttctcatcg ccgaagctcg tataaaactt
    28801 gggcaggttg atgatgccta tctttttgtt ttggatatgg agcacgttgc tccgggcgaa
    28861 ggtttcttcc aatttcagtt cttcccggac cagcttgagc acccggatcg tgccgtccat
    28921 tttcttcagg gtaagggaga ccaccgattt gtcggcccct cggatcagtt ttactccgtc
    28981 cgtggtacta aagcccgcga cgtccaccgg tttttcatcg ccctggccca ctttcaggat
    29041 aaagtccccg gcttccactt ctccggacct ccaggccggc cctccagcgg taacagctgc
    29101 aattttaagg tatccgtttt cttccccgat ctgggccccg atgccataaa attttcctga
    29161 tatgttttca tcccaggtcc gtttttccac tgggagaaaa taatcggtat gaggatccat
    29221 caggttgaca atgctgttga gatagaggct gaaaaattca tcatccgtat tttgatgaac
    29281 atattgttcc atcctttttt tggtggcctg cagcgccagg ttcctggctt cctgttcgag
    29341 ggtgatatcg gtccttgatt ttatggaatc cgcatcagcc tgttcccgga cttctttcag
    29401 gtccacatat ttttcgagca cctggtattt gattttgtcg gtccaggccc gggatcgttc
    29461 cgccattgtg gagggataag atttgcctgg atcgtaccat tcatccgggg aggctgcaaa
    29521 cacaaagggc ttttcaaacc agcttggaaa ttcccggctg agttcggcga cccgctcttt
    29581 atacctcgca agcacctcgt aaaaaaattg gacagtctgc ccgttcagtt catcatcaag
    29641 caggtccttg tatttttcga aggcggcgat gtccgactgc agaaagatgt ttttttcact
    29701 gtccatccgt ttaataaatt ccaggaagac ctccgaggaa agttcatcgt tgaagggacg
    29761 gggactaaag tgcccttgct ggattacctt ggccagggta tggagggttt ggtcgtattt
    29821 atctcccggt gcgtcggagt acctcagcgc gggcagggta aacagcacac tgaaacagat
    29881 gaaaacgctg accgtttttt tccagatggt tttagtcatg ataaacggat tatggctttt
    29941 gacaaatgta atggattctt aattgacggc ctccccgccg attcttaaga ggttcataaa
    30001 aaaaagagga cagcgacagg cgccatcccc ggaacgctct actttatatt ggatgccggc
    30061 aagtcccgga ctttgggact ccggatcaga tttgactaat ccctaagact tactttacaa
    30121 tcaggataca aacataagcc cggcccggac atgagtaatc acccgcttag ggggatttgt
    30181 gcgaatgctc cggatccgcg ggggcaaagc ctcccgtaat ttgcaattca attcaaatga
    30241 gcttatcctg cctggcaatg cgcagggctt cctccttgga attgacgtcg agtttgctgt
    30301 aaatgttttt gatatgggtt tttaccgttt ccttgtcgat aaaaagttcg gaggcgatcc
    30361 gggttctgct tttaccttcc gcaatgccct cgaggatctg ggtttccctt ttcgacaggg
    30421 gggattggga atttttacga aaggattgga tgaccatttt ggcgacgttg gcgctcaggg
    30481 gcccgccgcc ctgcaggact tccgtgatgg attcaatgat tttggaggaa ggggtgtttt
    30541 tggtcaaata gccggaggcc cccaggctga gcgctttaaa aatgttttgt tcattttcat
    30601 agaccgtaag gatgatgatg tgggcggcgg ggtatttttt cttgatgcgg gagatcgcat
    30661 cgaggccgtt gatgccgggc aattcgatat ccagcaggat gacatccggg aaatcggaaa
    30721 ccaggtatgg ttcggcttct tcgtaactgg cgtagctgtt gaccattttc agtccatccg
    30781 acaggttgac caggtaggtg aatccttctc ggatggtctg atcgtcttcg atgataataa
    30841 cgcgggtgac catgatcctg atttagtgat ataaaaatat ttggtactgt aaagttcgca
    30901 gatctgcgaa ataatttatc ccaccgtccg ggggatttta atcctgatcc ggatcagggt
    30961 cccctgcggc gtattgggga gaaactggat ctgtccgccg acccgtgcaa tccttttttg
    31021 catgttgtcg agcccgtacc cctttttcat ccccttgttt tccggcatcc caatcccgtt
    31081 gtcccggatc tcaacggtaa ggccggtgcc gtcaaagatg gaggagggct ggagactgaa
    31141 ttcaatggaa gcatgggtgg ccccggaatg cttaagaatg tttccaaagg cttccttgca
    31201 gatcatgatg agattccggc tgtattcaaa gggaggtttt atttcctcga aagaggggtc
    31261 cggcgggcag gatttgaaag tgatccgcga gtcctggaac agctctgtgc caaaattctg
    31321 tatacgtccc gcgacatcca taaggctgtt gttgcccggc gccagggccc agatgatatc
    31381 cttggtcccc atgtacaatc cctggatatt ttcccggatc tgggccatga gtttatgctg
    31441 ttcttcattg ccgccgagct tggtctgaag gatatcggtg agcagggaga gtcgggtgat
    31501 tttattgccc aggtcatcgt ggaggtcttc ggaagtccgt tgctgaatca gcaactgttc
    31561 ctgcatgcgg gcttctttga gcagccgctg ttgccgcgcc ctgagaacca tcctgatttt
    31621 ttggatgaca gcaccggtca acaggagaaa caggacggcc aacaacctga accaattggt
    31681 ttggtaaaag gggggtttga tgctgaacgg ataggccagt tcacttgaat aaatgggagg
    31741 gtcgctgtcc agcaaagccc tggcccggaa cgtgtaatct cctggagcca ggttgggata
    31801 gattacggaa ggaacggggt ccggtgccga atagacggta tccgccccgg acaggaaata
    31861 ttgatatctc agtttttcgg gagcggtgag atgcacggcg ataagggaaa aggagagatg
    31921 gttttgacgg tgcttgagtc ttagttccct ggggatgcgg taccaggcag ccaggctgtc
    31981 ggtgcccatg gtggagtccg ggagttggga atggatcgta agggatttta gaaagaggcg
    32041 tgggggaagc aggggcgctg ccggtttggg gtcatagata tagatgcctt tggtggtgcc
    32101 cgcccagatg cgtccttcgg gatctttcag gatcgcgttt tggttacact cagggcccag
    32161 tttctggttg gccgatttca atggccgcac ctgaaaggtt cccgaaattt tatcgaagcc
    32221 gactttattg agcccattga tggtacctac atacagcgag gccccatcgg ggagtacgct
    32281 gaaggtcata ttggaggaaa gcccgttttt ctcattgcag acataggatt taccggtttg
    32341 ggggtcccag accaccaagc cgttttccga agtgccgaag accaggaggt ctccccagcg
    32401 gttgatggat ccaatcacaa atccctgcgt accgggcacc tcgaagggtt tcagttcgta
    32461 ttggcggttg atgctgaaca ggccttgggc cgtgcccagc aaaagggtgt ccgggttgat
    32521 ttcaaaggtt gaaaaacaag ataaatagac gccgggcata gggatgggaa gtccattttc
    32581 tatcctggac accgtcatcg ggctggttaa ccagatgacc ccccttgaat cctggttgat
    32641 ggaagtgaaa tgtcttggga atctgggggt atcccagccc acccgttcga atttattatt
    32701 gcggtacctc cacaatccgt ccccggaagt gccgatccaa acggattgcc cggcatccac
    32761 aaatacacag acgatcttgc tggcttcggt ttttccggta ggtaacttga tattacggat
    32821 ggttttatcc cggatcacgg aaaggccatt ttcaagggaa cctgcccaat agttgttttc
    32881 atggtccctg gccaggccca tgatgatgct gccgctcagt ccgctccccc ggtcatagat
    32941 tcggaaggat cggtcatgaa atttaaagat gccgttgccg tcggttgaaa accagagatt
    33001 ttcttcccgg tcgcggaaaa tatggaagac cgcattgtcg gtgaggccct gcagaagccc
    33061 gatgtgttca gcgctttggc cagggctgag tttatatgcg ccccgggtgg tgcccaggta
    33121 aagatcgccg gccggatcct cttccaggca aagcacgggt gaagggatcc ctgtgttctt
    33181 tttaaccagc ctgccctgtt cacaagaaaa caatgctccc tgcccgatta cccaaagacc
    33241 cttgacggag tggctccaca tcattttccg gatcactata cctgaatccg ctggagtgaa
    33301 tggacaaaaa acctgccatt gtcccccgga ccatttatag attccttttt tcgcaaaggc
    33361 tgcatagagg cctgaagtat ccgcagcgac agccgtgacc gacacggtcg tatccgaaaa
    33421 ggcttcgagc aattggctgc ctggatttaa tttgaacaac tgccagccgc tgaggagggc
    33481 tatgatgctt ccgtccggcg cctgggccag gtcattgaca tgtcttctgc ggttgtccgg
    33541 ggcgatgccg atggtcctga atcctttccc gtcataggcc tgcagccctg cttccgtgcc
    33601 gatccaaacg gtgttttggt cgtcgacgaa taccctgctg gtcagggagt gcatgagccc
    33661 attgccgtaa ttaaaattgg taaaggtatt gccatcgaag cggctgatcc ccccataggt
    33721 gctgatccag agcatgccgt attggtcctg ggccgtattg ttgacctggg attgaaccag
    33781 gccttcttcg atgctgtaat tgacgaaatc atatttctgt cccggactaa taaggggcca
    33841 ggccagggcc aacagggcgg gaaagaaaag aaagaatcta tgtatcagtc cgggaaacat
    33901 tggattttga ccggtggaat tataatatta tcagaacagc cattacaaaa atgccgtttt
    33961 tccgattttt tccatcaccc caaaagggtg aagggcctcc ttgcagggac agagaccatc
    34021 atttttattc atttcgggtt ggtatgccgg caagtattgt cttatgtaat aagattttaa
    34081 tgtataaatt attaatattt aattactttc tgattggacc attaaattca tcgcagtttg
    34141 agacttatcc gtcgggatcc tgtgaaaatc tgcacttcag aagcaaatca taatcaggga
    34201 aaaacctgac attaggttag ctttatttaa atgaaatcgg taactttaga tctgtagaaa
    34261 aatcctaact attttatcat cgtggttgcg gaaccaagat catttttata attttacccg
    34321 aaaacaaccg catatgaaca agatatttac atttattttg gcaatgatca tgatgggcgc
    34381 ttgcgtaagc aaaaagaagc acgacatggt taccaaagaa cgggattcca ccgctgaaga
    34441 gaaacgccag ttgatggctc agttaaaaca gagccaggat caataccagg acctgatgaa
    34501 aaaatatcag gacgaacaaa acaaactgaa atcggatatg caaatgtccg aatccaaggc
    34561 catggagctt caaaaccgca taaaaagtct ggaagcccaa ttggagatgg ccaataaaac
    34621 caataccaat ctgctctccc ggctggaaga catgtctatc atcagcaaag ccggtgcgga
    34681 aagcatgcaa aaatccatcg atgccatcaa tcagcagtcc aaatacatcc agggcctgaa
    34741 taccaagatc cagatgaagg attccctgaa tatgcaattg gtcatgaacc ttaaacggtc
    34801 cctgggtgat atcaacgatc aggatgtcca gatagaggta aggggaggcg tggtatatgt
    34861 ttccatctcg gacaaactgt tgttccgttc aggcagttac gatatcaatc cggccggcga
    34921 ggcggtgctg ggcaaggtgg ccaaggtggt caatgaccac aaggaaatcg atatcctggt
    34981 agagggccat acggataatg tgccgattgc tacggataag atcagtgaca actgggatct
    35041 cagcgtcctc agggccactt cggtcacccg tatgcttcag aagaaatttg gagtcgaacc
    35101 caaccggatg accgccggag gaagaagcga atacgcaccc aaggattcca atgacaacag
    35161 ggagggccgt caggctaatc gtcgtacaga aattataatc actccaaaac tggatcagta
    35221 catcaatctg cttgctccgg ctgagaagtg atctcatcag gacgaatttc cagaatactc
    35281 aaaatgctgg tagtcttcgc aggctatcag cattttttat ttggacaagg gccggtacag
    35341 acgggtatca cccggacttt ttcaggcata ccgattccgg aagtattgga taccctggct
    35401 tcagggtatg gattaaagat ttactatctc ccttcggaaa acattccgag ggacctgttt
    35461 accggttctt tcaacaatca aacagccgat gaagcgatca gaacggtatt gtcaaaatcc
    35521 agcttgcagg tgatcgccgt cagcgaccgg attttttttc tcacctcggc cctggggcgc
    35581 gaaggagtga atatgaaccg gtatctgtcc acgaaagcaa aactggaaaa tgaatacctt
    35641 ccgggcgggc tggttctggg ccacccggac tccatgccga tccaagggcc tgcccggata
    35701 ctgggttgga tcaggaacag cagggatgaa accccggtcg agggggcggt ggtacagatt
    35761 ggcgatgctg atgcggtcac cgattcagcc ggcaagttca cannnnnnnn nncacaatcc
    35821 tgaccctgcc cggtacttat tcgcttcggg taatctctga ccggttttcc gaatttaatc
    35881 aaaacctcaa agtctttggt gacgatcatc tcgagctgaa cctgtttgaa agtacggttg
    35941 atttgcctga actggtggtg ggggcgaatg cccgccaaaa attacaagag gccaccagcg
    36001 gcctttccca attgagtgta aaagaaattc aacggcttcc ctccctgatg ggagaagccg
    36061 atgtagtgaa ggccctgact actcttcccg gggtgagcac caccggggaa gtgggatccg
    36121 gataccatgt acgcggaggg aataccgatc aaaacctggt tttacaggac ggagtggctt
    36181 atttgaatac gtcccacgtg cttggccttt tttcttcatt caatgcggat gccatcaata
    36241 atgtgtccct ttacaaaggg catattcccg cccggtttgg aggccggatc tcttccgttc
    36301 tggatatcca gcttaaggat cccgatatga aagcctggaa tttccaggga ggcctcggcc
    36361 ccatctcttc gaaggtatat cttgaaggtc cggtcgttaa agacaagact tccatgctgg
    36421 cgggactccg tttttcgtat tccgactgga tcctcagatg gttgcgtgac ccgaacctga
    36481 aaaacagcca ggccgatttt tacgatttca gtgttaaggt caaccaccgc tttaaacccg
    36541 gccatgtgct ctccctttcc acctacgcca gtaaggacct gtttaattat gccgatcaat
    36601 tcggatatgg ctggcaaacc ctgtctttct ccgcccagtg gtatttggac ctgaaacccg
    36661 gaatgagcct gcacgcttca gccggcagga gtagtaaccg caatcatttt accgaaacgg
    36721 atcaggacaa aacagaatac cggattggga ccggcctcgg gttcaacaat gcaaaagcaa
    36781 gactggcctg gcaaatcagc ccatcccagt tttttcaggc cggggcggaa tggaccggat
    36841 atacgccgga tgacgaaaat taccgggtga aaccggccag tgatgcggcg acgaatgaat
    36901 attatccaaa aaccaggggc cgggaggccg ccgcttacat ccagctagaa agcaggattt
    36961 ccccgaaact taacatatcc atcggatacc ggcactcact ttattggcaa gccggcccgg
    37021 ccaggatccg gatgtacagc cccggtcagc cccggacgga agaatcggtg accggaacga
    37081 aggattatgc gaaagggtca gggatcaaga cctaccaggg tttcgaaccc cggctgagcc
    37141 tggcttattc caccgggccg cagagttcca taaaactgag ttataataaa atgaaccagt
    37201 acatccacct gatttcaaac acggccgtgg ccacccccgt ggatatctgg cagttgagca
    37261 atcaacacat cgcccctcag cgcggaaaca attattccct tggttttttt aaaaacctca
    37321 aggatcatcg ctgggaaacc tccctcgaat tttattttcg caattccagg aatgaactgg
    37381 aatacaagga ttttgcacgc ctgctcagaa acgaattcct ggaaacggaa attttcgcgg
    37441 tgaaaggaag gtcatacggg gccgaactgt ttataaaaaa ggcagttggc gatttcagag
    37501 gttatctttc ttatacgctc aaccgtagcc ttcgaaaatc tgagggcctg tatcctgatg
    37561 aagtgatcaa tcacggagcc ttttatgctt ccaatttcga caagccgcat gccatcaatg
    37621 ccctctttga ctggcagatc cgaaaaacct tttcctggtc ggcccgattt gttttcaatt
    37681 ccggccggcc tcagaccgcg ccggtcgcgg agttttacct gggcgacggg gggcattttt
    37741 ttgattactc cgaaagaaat gaattcagga tcccggtcta tcaccggctg gacctgtccg
    37801 tgaccgtgac gagggggaat atccggacca ggaaatacaa agcgagttgg accttttcaa
    37861 tatacaatgc ttacaaccgg agaaacgcat tttccgtttt ctaccgcagg aacgtcaacg
    37921 aacccccggt ggcttataaa ttatcggtat tgggggccat tttgccatcg gtaacctata
    37981 atttccgttt ttaatgaacc ggctgtccat tggcatattg gtagttcttc tgcttgctca
    38041 actggtctcc tgtatagagg agattccgtt tgcctcaggc aaaggattcg ggactttggt
    38101 gatcagcggc gggataaaca acctggagga agaccagaag gtctttctat ccagaacggt
    38161 tgaaaatggc ggcccccccc ggcccgaggc ccacgctctg gttaaactgg tggaatccac
    38221 cggccgctcc tgggatttca tcgagaccag cgagggccag tataccctgt ttgccggcac
    38281 cgccgatacg gaattcggag caagctacca tatcgaatgc accctggaag ggggtgtccg
    38341 ctatcagagc ctaccggaaa agttgtttcc ggcagtgcaa cccgacagca caggctggca
    38401 gatcggtaaa gaaaccacaa ggattgagga tggttattcc cattccatcg atgctttcca
    38461 gttgtttatt cataccgggt ttgcggccga tcagccggaa acctatttga ggtggactgt
    38521 gcaaagcgcg tttcaattca ccaccttacc ggaatgcagc cccttccgga ctacggtaac
    38581 ctgctatttt tccgagctgc ttaatccggg caggttttat atccgtagca ataaggacca
    38641 ggcggggggg gtattgcgaa atcaccccat tggttatgaa tccatggaac ccttttaccg
    38701 gtattcagag acccattatt ttaccattta ccagcaccgg atttcacaaa gggcctatga
    38761 atattattcc aggctcaacc aggtggccgt gcaaaacgga agcattttcg atcccatccc
    38821 ggcatcggta aaaggcaacg tgtaccgggt ggaccgggag gatgaaaagg tgttgggata
    38881 tttccaggtt tcttcgattg ctgtcgtccg gatgaaagtg gttcaggcgg acctggcggg
    38941 caaatataac ctcctggaca aaaggtataa tttatgcgga ctgtatttcg gttaccccga
    39001 tgcggggtat tttgaaggct gctgcaattg ttattcgatc aaatttccgc tcatcgataa
    39061 accggactgg tggtgaaaaa cagctaccgg catcttatct ttttgatgtc attgctcttc
    39121 gcaggccggg aagggtattg ccagggattc ggtatacacc tggaccggaa catctatatc
    39181 tccggtgacg cgctgagatt caaaatctat tatctcgacc agctggatag gatcccgggt
    39241 ttgctctatg tgcgtttact c
//
LOCUS       DAOQXT010000038        39056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1202_length_39056_cov_6.848773, whole genome shotgun sequence.
ACCESSION   DAOQXT010000038 DAOQXT010000000
VERSION     DAOQXT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 39056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39056
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1202_length_39056_cov_6.848773"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            820..2616
                     /locus_tag="PKM27_08520"
     CDS             820..2616
                     /locus_tag="PKM27_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNR07345.1"
                     /translation="MKAIYALLVGIDSYNPPVPKLSGAKKDIEKIKTYLEKNFNDRPL
                     HIKTLLEQEATYDQFIQNFRDHLGQAKADDVVWFHYSGHGSRQTSAEEFEKLNSGKKD
                     ETLVLYDSRPSGKDLADKELAILIHEIGTRGPHILVTLDCCHSGSGTRSAEAFTERLS
                     ADRGDKRTIDSYLNGYFNQRGTTLPEARYLFFAACNRFQSAKESFDGGGLFTENLIQT
                     LEKSKKDISYADLLIRLRQGVVSMKWDQDPQMEVVGDLSSYTQFLDGSVVKGAPKYAV
                     RHDEEGWAMEAGQILGITEEVKLAIYPDQSNEPLGAAIVNQSSAQISRISTESALDTD
                     AKYWAVPLNIPGIPFKTGLNVDEHFREAFVEEAERFLNVRFNIPGNENDPYTLIQKEG
                     YFELINHENGLTILRADIKFKQAIHELVRKLDQIATWTRFLRLQNNKTALNPDCCSLS
                     LDIEVSPGRMQNFPPGEIRLKSDGSRIPYKLWIQNQFTQPLHFALIYLSDHYGSVPIK
                     NEPLEKSDQPTLFWGGTENDVFMLPEGSVSSKDSFLIIISTERTDDFQFTLEEMEFGD
                     LKFDDRAIPGMNRSTKVKGEWFTSRFMVILER"
     gene            2699..7399
                     /locus_tag="PKM27_08525"
     CDS             2699..7399
                     /locus_tag="PKM27_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR07346.1"
                     /translation="MPFKPVLFLAFANDKVDQTAYLRNLSAEMHGIRKALGEAVSSGL
                     CEVVERSSASVGDILDVFQDPVFASRISLFHYGGHANGFSLLLESEDGTKALAHKEGL
                     VPFLSRQENLKLVFLNGCSSEGQAADLLKAGISAVIGTTSAINDEVATSLAIRFYSSL
                     GDGSGIEKAWADATDEVKISKGTGNTRALLWEGKNEASEHFPWVIRFRPGAEIIKEWN
                     LPDISGNPLYGLPDIPPSFNLPESPFLYLNRYERRHAEVFFGRSYYIRSLFSSVHEAQ
                     APPIILLYGQSGVGKSSLLEAGLQPRLESRFEILYLRRSGALGLSATVYNALLEKCGF
                     KPEVSTISMATKDSDRVLDELRNIAANIEETFKPDVQQLIQKIAGTNATDTGSTTGGE
                     TPPSTLSEAWKQAEAKLNKPLVIILDQLEEAYTRPNERLGNEMNQWFIHLKAIFGNPA
                     LMPQGKIILSFRKEYHPEIEEYCKNYELPRSKIFLEHITKRDVLDVFRGFMETPRLKS
                     RYNLQVEDGLPEIIAADLAADKDSPIAPMLQILLTKLWIKSLTSNPEAPAFTHALYRD
                     LKEEGLAMDEFLQNQLNALKKKFPELHHNGFALEVLGFYCTPNGTSAARTEQQLVENY
                     PDAVPEIRNVLAGCMELFLITDLGGVERSAMLAHDTLAKSVVKLQQRSARPLQQARRV
                     LHSRMEAVRGGSASSTLDHWDLKLIDGVLKYLPAVSGEEKNLIEASRKAVLKKEKEKV
                     LLKYSKRGLAAITGIGALLIGLLYFQSRAHAKASFVNHMAAASAINLRKDPTLSLMQA
                     AEGMKVNQENSIAEIKKSLTDAFYSAVQNRTPWYDTVFSTDLNYYDLVFHENEKTMIP
                     FTETDSLEILDYDGNLLGALVTDPLSPDDPAMAGAPEDAFAYFTGLKWNRDGHSIVAL
                     NSDGILQFHEASGSLKKRIGMAHEYQAFDVSPVLNQAIAYAIGDSQGYAQDQIRLFDM
                     DGNLLKSFGTTADIKDIRFTADGKNVLLTRFDTLLTAIDQAGLPQNPEPDSWKKVEIL
                     NLDGMRIRQLDAKEGEVLDLWTSPSGKWLATVHAGGTLVLWTSEGRRFNTFKPPVLPG
                     RSGMPGTRENEILHIDFHPNDSLMAICFRQDAACLYPIYGKSAAPVMLWHEHLVTFAR
                     FSEDGRRVLTGSADNTAAVWDLKGNQLYHLLGHKNDVTGGKFIDQGEQVLTTSLDGTV
                     RRWVLENYPDLQFTGHRGAVTYIDLDPLDQYLVSAGVDHTMRVWDIKSKREVDRLDLG
                     DQGVRYAIFTGENEILGMGVKNQAFLYKLFEATPVKFIGHTGPVEWAGAIGDHIVTAS
                     KDGSIRYWNKTGKEVKRIEPGQGEMLSLIVSQQDSIVAAGSESGTIFLFDEKGEVIAE
                     LEGHRDAVIYLNVSDDGSRLVSTSRDGTAIIWDLKSKSEVARLDRIACAPYQDCQVNS
                     ASFSSDGSRIVTTSSDRTIRLWDSDGNLKASMVGHTGEVVDAFFTPNDDMIYSFSADQ
                     TLRLWDFQGKEIIAYRGHTGKINCAYMDFDMNQIFTASEDGTIRAWLSPIGVYRWMRD
                     SKQFKPIFHGKYNQ"
     gene            7380..8720
                     /locus_tag="PKM27_08530"
     CDS             7380..8720
                     /locus_tag="PKM27_08530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNR07347.1"
                     /translation="MENTINSWNGLNLDDLRQVMDPDADAAISSLYKSRAFNSLRDML
                     RDMARNDNFVPDSMPAGIRDFLTAELQFVPSAEDIAAFERAHRVWKENGVQFIFILFF
                     RSLPYTYMAEKPANVLRMTKILEAHPQRRIFETAQFVFDVSDKAWWTPEKRGLITALK
                     IRLMHAAMRYNLLYNPEEENWNKEAWGMPISQEDLIATNQVFSLEFFKGMAILGQALD
                     EEDQNAWFHTWKTIGKIMGIQDNLLCKTKEEAWTLQKAMYNHLFPDDNRAGIALAKAL
                     VETLATFLLSKKLILLMMKKMILDEDHPDLFYRVLGPSFAKEYPSVFKKSRSVEEAEE
                     DAAAEHQEFYDELMTYKEKVRSYRENQKQKDPVSRGEGNKNLVDLQLDVFEELAEKMD
                     PKGPVDRGIVHDLFKTAMSSVGGVIIGILSLYFREGKEAGFRIPTDLKEYWALS"
     gene            8758..9618
                     /locus_tag="PKM27_08535"
     CDS             8758..9618
                     /locus_tag="PKM27_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR07348.1"
                     /translation="MTDLQRSIIRYYDATLLDYRAFWVGKRDLAFHFGYYDTSKESHR
                     QSLENMNRVMAGKAGMDASDLVLDAGCGQGGSALWLATHIGCVIQGISLVPHQVEMAN
                     RVAKARGLENKARFYIQDYAKTGFPEESFTVIWACESLCHAPEKKAFYQEAFRLLKPG
                     GRLVVAEYIRKGRNNAVQDENILQNWFSGWSMPDLDTWQEHWDHMREAGFTRIQHEDI
                     TGHVSPSLHRLFKMSKRLLGLGKFLHLVRIRNHIRHRNQVASISQYVALTKNLWNYYI
                     YTASKPGLPK"
     gene            9615..14861
                     /locus_tag="PKM27_08540"
     CDS             9615..14861
                     /locus_tag="PKM27_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR07349.1"
                     /translation="MTEHGLYIPDGYVLLSGIYRDDVHEILKAKRKSDQREVVLKVSR
                     PSNNDILKISKLSHEYHTLKNLDHEGVIRVLGLHSKNKSVCIVEEFFEGETLKSRIFN
                     QAFTVPAFFQVALQLTDILAYVHSQGVIHKDINSSNILISKDNKVKLIDFGISTHIMN
                     EEQDMVIPDLIEGTLTYISPEQTGRTSYSISAGSDLYSLGIVFYEMLSGKPPFDSADA
                     LEVIHFHLSRTPAPLGKVLPRLPSGLEKIIAELLEKNPDDRYQSAGGLHHDLKLLQGE
                     IESGKASSFFKTRTNDKGGRFRKAQRLYGREQEVASIISCYENLHQTRTMLVLVSGYS
                     GVGKSAVVKQLQRPVTDKNGLFISGKFDQFKRNIPYFAFIEAFDNLLRGLLAESDDKI
                     KAWKEKIWEKLGANASLISEVIPSLEFITGKLPLPEKLPPAEQEFRFRLVLLDFIYCF
                     STTDRPLVIFLDDLQWADLPSLNLIERILSLRRSENLLIVGAYRSNEVNELHPLSMTI
                     HQIKGEQVKVMDIRLQPLDQNTTIQIVADSFGMPEAQATDLGLHVYAKTKGNPFFINR
                     FLQSLYDNKYLTLDASRKWTWDRNKIESLSYTDNVIDLMANEMTSLPETSREAMKYAA
                     VLGNTFNLSTLSMVMEKNQNQVYPSLLPALKGGYILPLDQNYRALSLYTEGINEEFET
                     ESENLFINFRFLHDRVQQSAYGLIAPGDRETIHFRTANLLYSKSTPEKLNDFLFDIAN
                     HFSLSKNRVFEQGDRRSIAALYLNAGSKAKDSTSYDVAIKYLSAARDLLPEQSWQSDY
                     ELTFAVYSALGESEYLNSNHEKAEYLFEQILKYARTNLEKLKIYYVHSALYLKIGNTK
                     KSLELGREAMKLYNIHFPESEWAIKSKATWELAKYLFLFSTRYRNVEKLYHLKDCTDQ
                     EIIAINQFLIDIATSAYQENQNLMVIIVLRIIRHYLKYGFTDASGWGFSGFSVIAYSG
                     IGLSKRGLGLWSITRKLHQRTRSAIVRTKLDYTVNAFYTQWTKPLTENLDEIINNVKA
                     CLMNGDPNFAGYSIALQFWKKSASGLKLTEVLDSTGEYLEYLKNNKNVTGLNYIIPSV
                     QAVKALIGKTPVNGDWNSEGYDEPAFAQQLNAVGNKTNIAFFYNFKLPLYYYFDKYEE
                     GLQWAEKGDALQAYILGHYFLSEWYFYYNLLIASLYEKFSTADKSKYLKIFRRNSKWF
                     RHWIKGCTENFEQQLYILKAEEMVMEGQVENSIRWYEKAIEAALSHGFTQFAAIANER
                     AAKHLAGKGILRQSAFYLKAAYEWYEDWNAFGKCRQLKGARPDLFGDKIIQITGNTRT
                     NLPTAALDYGSLIKASNSISSKIKLDELIQRLMNVLLENAGADRGVLMIPRQGELFII
                     AEGKSGPDNIQGNIQVPVKGSNKIPQSLINYCWNTQETKALSNALRDEAYSNDPYVME
                     HQVRSMIGIPLSNKGNKVGLIYLENKLLEGVFTSNKLEVLNMLSGQIGISIDNALLYE
                     TLESKVTERTRDLEKQKQIAESQTREAIQQKMLADEERKKSDALLLNILPDEIAQELK
                     LKGESQARQYHSVTVIFTDFVNFTTVCEYLPPAELVAELNKCFAAFDKIVDGHGLEKI
                     KTIGDAYLAASGLPRINPDHATHACRAAMDILNWVRAPENKCLFNIRIGINSGPVIAG
                     IVGMKKYVYDIWGDTVNTAARMESSSSPGKINISESTYQLVKEKFNCVCRGKIEAKNK
                     GSINMYFLEENPLPGVLTTAVG"
     gene            complement(15211..15930)
                     /locus_tag="PKM27_08545"
     CDS             complement(15211..15930)
                     /locus_tag="PKM27_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNR07350.1"
                     /translation="MLEKLTRLQNYLSQDFLGGPRLIKLSALINFQKGGTLPLVLFLM
                     YWFDNYSAEAWVYAGLHGSYGICWLIKDRYFPDRRWDIKITLGGAFMSFALVLGLYWL
                     FPLILISPWFHEEGFALASWFVGLCIFIHTMGVVIMMTSDAQKYYSLKYNPGLITEGI
                     FRRTRNPNYLGEMLIYATYALMASHWLGWAVLAWVWSGYFLVNMILKDRSLSRHEGWE
                     AYKKQSGILFPTIPGFKSRHK"
     gene            complement(15999..16454)
                     /locus_tag="PKM27_08550"
     CDS             complement(15999..16454)
                     /locus_tag="PKM27_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR07351.1"
                     /translation="MNSSTFVRKASLEDLASLLDLVRELAVYEKAPEAVTATLQDYEK
                     NYLEGVFDALVAVDREGAIVGTAIYYVCFSTWKGRMLYLEDFVVKESLRGLGTGRLLF
                     DALLQEAVNLDCRLVKWQVLEWNEPALNFYGKYQAIIEKEWWNGKIFVG"
     gene            16575..16949
                     /gene="rpsL"
                     /locus_tag="PKM27_08555"
     CDS             16575..16949
                     /gene="rpsL"
                     /locus_tag="PKM27_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNR07352.1"
                     /translation="MPTINQLVRSGRKQVKAKSKSRALDACPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRMTNGIEVIAYIPGEGHNLQEHSMVLVRGGRIKDLPGVRYSIVRGAI
                     DTAGVNNRKKSRSRYGTKRPKK"
     gene            16980..17447
                     /gene="rpsG"
                     /locus_tag="PKM27_08560"
     CDS             16980..17447
                     /gene="rpsG"
                     /locus_tag="PKM27_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNR07353.1"
                     /translation="MRKRKPKARAIQGDPRYNDTLVSRFINNLQWSGKKSGAQSIFYD
                     AMDIVKAKNAEENELEIWRKALDNIMPHVEVRSRRIGGATFQIPTELPANRKVFFGMQ
                     WMIRYARARAGKGMAEKLAAEIIAAQKGEGAAVKKKEDIHKMAEANRAFASFR"
     gene            17477..19615
                     /gene="fusA"
                     /locus_tag="PKM27_08565"
     CDS             17477..19615
                     /gene="fusA"
                     /locus_tag="PKM27_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR07354.1"
                     /translation="MAAKDLRYTRNIGIAAHIDAGKTTTTERILYYTGITHKLGEVHD
                     GAATMDHMEQEQERGITITAAATKTAWNWNGDEYTINIIDTPGHVDFTVEVERSLRVL
                     DGTVALFCAVSGVEPQSETVWRQADRYKVPRIGFVNKMDRSGADFFNVVDEVRTKLGS
                     RPVPLQVPIGSEHNFKGVVDLITNKAMVWNESDQGMTYTEIPIPEDLAGTVATWREKM
                     MEAAAEYDDDIMQKYFDDPSSISVKELQAALRKAVIDMKIVPMMCGSAFKNKGVQAML
                     DAVCTFLPSPADVDAVEGINPKNDQKELRKPLNSEPFAALAFKIVNDPFVGRLSYMRV
                     YSGRLENGSYILNTRSDKKERISRLYQMHANKQNPIESVEAGDIAAAVGMKDIRTGDT
                     LCDPDKPIVLESMKFPEPVIGLAVEPKTQKDLDKMGLALNKLAEEDPTFKVRFDEDTN
                     QTIISGMGELHLEIIVDRMRREFGVECNQGAPQVNYKEALTRTVSHREKLKKQTGGSG
                     LFADIEFEIGPADEEFLQSEDFKSGKTKMQFVNDIVGGSIPRELIPPTQKGFLSMMEN
                     GVLAGYAIDSMKIRIFDGSTHTVDSKPVAFELCAKEGFRAAAPKAGPVILEPIMKLEV
                     VTPEEFVGPVIGDLNRRRGMPKGQELRPGSAVAIQADVPLSEMFGYVTQLRTITSGRA
                     SSTMEFSHYAQVPSGIATDVIDKAKGAVKA"
     gene            19653..19961
                     /gene="rpsJ"
                     /locus_tag="PKM27_08570"
     CDS             19653..19961
                     /gene="rpsJ"
                     /locus_tag="PKM27_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNR07355.1"
                     /translation="MNQKIRIKLRSYDHNLVDKSTEKIVKTVRSSGAVVTGPIPLPSE
                     REVYTVLRSPHVNKKAREQFQLRTHKRLIEIYTPTQKTVDALSKLELPSGVDIQVKLT
                     "
     gene            20072..20695
                     /gene="rplC"
                     /locus_tag="PKM27_08575"
     CDS             20072..20695
                     /gene="rplC"
                     /locus_tag="PKM27_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNR07356.1"
                     /translation="MNGLIGKKVGMTSIYDDAGKYIACTVVEAGPNVVTQVKTDDTDG
                     YFALQLGFGEAKPKNTSQQMKGHFEKVKTTPKQKLVEFRDFPLAKAAGDKVTVDEVFK
                     PGDTVHAVGTSKGKGFQGVVKRHGFGGVGELTHGQHDRQRAPGSVGSSSYPSKVFKGL
                     RMAGRTGGERVKVRNLKVVKIFPDQNLLVIRGAIPGHNGSYVIIEKK"
     gene            20699..21337
                     /gene="rplD"
                     /locus_tag="PKM27_08580"
     CDS             20699..21337
                     /gene="rplD"
                     /locus_tag="PKM27_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNR07357.1"
                     /translation="MKVSVFNTQGADTGRQVELPEAIFGIEPNKHAVWLDVKAYLANQ
                     RQGTAETKERNLISGSTRKLHKQKGTGGSRKGDIKNPVFRGGGTIFGPNPRDYSEKVN
                     KKVKTLARKSVLSDKAAQGKVMVIEDFSFDKPRTKELSAVLKNLKVLDKKALFVIPDY
                     DQSLILSGRNLPKTRLLHARDLNTYSILHSNVVVVAESALPKLEETLLSTAK"
     gene            21346..21642
                     /gene="rplW"
                     /locus_tag="PKM27_08585"
     CDS             21346..21642
                     /gene="rplW"
                     /locus_tag="PKM27_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNR07358.1"
                     /translation="MEKKILIRPIISEKAEKLSSAQPRYTFLVNKDANKIEIQKAVQQ
                     HYGVTVKDVNTLTNPGKKKSRSTKRRVSVGIKPSYKKAVVTLNEGDSINIYGEI"
     gene            21660..22502
                     /gene="rplB"
                     /locus_tag="PKM27_08590"
     CDS             21660..22502
                     /gene="rplB"
                     /locus_tag="PKM27_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNR07359.1"
                     /translation="MPVRKFKPVTPGTRFRIANTYTEITKDVPEKSLVKALTDVAGRN
                     NQGRMTMRYRGGGHKRQYRVIDFKRDKDGVPATVHSIEYDPNRTAYIALLHYADGEKR
                     YMIAPNGLKVGQKVMSGKDAEPEVGNTLYLRDVPLGTSIHAIELHPHQGAALARSAGT
                     YAELTGKEDRYAVVKMPSGEVRRILIQCRATIGTTSNPDHSQTILGKAGRSRHRGIRP
                     RNRGVAMNPVDHPMGGGEGRASGGHPRSRNAKKAKGAKTRNKNKKSEKLVISHRNAKK
                     SQGK"
     gene            22514..22792
                     /gene="rpsS"
                     /locus_tag="PKM27_08595"
     CDS             22514..22792
                     /gene="rpsS"
                     /locus_tag="PKM27_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNR07360.1"
                     /translation="MARSIKKGPYVFHKLLKKVSVNKQANKKTVIKTWSRASMIIPDM
                     VGETIAVHNGKTFIPVYVTENMVGHKLGEFSPTRTFRGHSGNRKDEGK"
     gene            22821..23222
                     /gene="rplV"
                     /locus_tag="PKM27_08600"
     CDS             22821..23222
                     /gene="rplV"
                     /locus_tag="PKM27_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNR07361.1"
                     /translation="MEAVAHLRNGHGSARKIRLVVDNIRGKKVVDALNILKFTKKDSA
                     YWVEKALISAVANWEIKSEQSGNTDAYDLVISKAFVGGGPVLKRFQPAPHGRAHRIRK
                     RSNHLTLVVSNSVPLPSEAQQEPEAQEAQEA"
     gene            23253..24059
                     /gene="rpsC"
                     /locus_tag="PKM27_08605"
     CDS             23253..24059
                     /gene="rpsC"
                     /locus_tag="PKM27_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNR07362.1"
                     /translation="MGQKVNPIGSRLGIIRGWDSNWFGGKSMAKKVVEDEQIRKYLFA
                     RVEKGGIAKVVIERTLKRITLTIHTSRPGIIIGKGGTEVDKIREELKKLAGSDVQINI
                     MEIRKPELEATIVGESIAKQIESRINYRRAIKSSIQSTMRAGAEGIKVRISGRLNGAE
                     MSRSEEYKEGRTPLHTFRADIDYALAEAQTVYGKIGIKTWICKGEILTKRDLSPNIGL
                     VKKDSAGGDRRDDRGGRRRPGGGGRGDRGRGERGGDRDDRGGRGRRNNQD"
     gene            24108..24521
                     /gene="rplP"
                     /locus_tag="PKM27_08610"
     CDS             24108..24521
                     /gene="rplP"
                     /locus_tag="PKM27_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004934537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNR07363.1"
                     /translation="MLQPKRQKYRKQQKGRNRGVAVKGSTISFGSYALKSMELGRITN
                     RQLEAARIAMTREIKREGQVWIRIFPDKPITKKPAEVRMGKGKGAVEYFVALVQPGRI
                     LFEMDGTSTEAATKALNMAAQKLPIKTKIVIRHDL"
     gene            24533..24793
                     /gene="rpmC"
                     /locus_tag="PKM27_08615"
     CDS             24533..24793
                     /gene="rpmC"
                     /locus_tag="PKM27_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNR07364.1"
                     /translation="MANKKNQDIKEMSPTDISNSLIETRIDLQKARFTHAVKGLDNPK
                     QITGMRKKIARLKTEERARQIQAMTEAEKAGRSKIRARRSKS"
     gene            24826..25110
                     /gene="rpsQ"
                     /locus_tag="PKM27_08620"
     CDS             24826..25110
                     /gene="rpsQ"
                     /locus_tag="PKM27_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNR07365.1"
                     /translation="MEQNSEVKEKAPRKIRKTRVGVVTSAKMQKSITVSLDIKVMHPK
                     YGKFLHKTTKFMAHDEKNEAREGDTVKIMETRPLSKNKSWRLVEIIERAK"
     gene            25151..25519
                     /gene="rplN"
                     /locus_tag="PKM27_08625"
     CDS             25151..25519
                     /gene="rplN"
                     /locus_tag="PKM27_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNR07366.1"
                     /translation="MIQTESKLNVADNSGAKIVQCIRVLGSTRRRYAGVGDVIVVSVK
                     DATPTGGIKKGTVSKAVIVRTTQPIHRKDGSYIRFDDNAVVLLTNADEPRGTRIFGPV
                     ARELRDKNYMRIISLAPEVL"
     gene            25544..25894
                     /gene="rplX"
                     /locus_tag="PKM27_08630"
     CDS             25544..25894
                     /gene="rplX"
                     /locus_tag="PKM27_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNR07367.1"
                     /translation="MITKSKNKIRRFAPKLNLKKGDQVIVISGDQKGKKGEVQKVMKE
                     TLKAIVTDVNMVKRHTKPTNNNPGGIREIPAPIYISKLMLLDPKSGEPTRVGRRTENG
                     KSVRYAKKSGQTIK"
     gene            25902..26459
                     /gene="rplE"
                     /locus_tag="PKM27_08635"
     CDS             25902..26459
                     /gene="rplE"
                     /locus_tag="PKM27_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNR07368.1"
                     /translation="MKYTPRLKQQYTEEVVPKLKEQFNYKTIMQVPRLMKICINQGVG
                     EATQDRKVVDIACTEISQITGQKAVPTKSKKAISNFKLRENMHIGARVTLRGDKMYEF
                     LDRLISVALPRVRDFRGINDKSFDGRGNFSMGITEQIIFPEIDLDKVNKISGMDISIV
                     TSARTDKEAFALLSSLGIPFKNQKN"
     gene            26474..26743
                     /gene="rpsN"
                     /locus_tag="PKM27_08640"
     CDS             26474..26743
                     /gene="rpsN"
                     /locus_tag="PKM27_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNR07369.1"
                     /translation="MAKKSVIARQKKREELVAKYAETRAKLKAAGDYEALDKLPRNAS
                     PVRLKNRCQLTGRPRGYIRKFGLSRVAFRDMALYGKIPGMTKSSW"
     gene            26772..27176
                     /gene="rpsH"
                     /locus_tag="PKM27_08645"
     CDS             26772..27176
                     /gene="rpsH"
                     /locus_tag="PKM27_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNR07370.1"
                     /translation="MAVTDSIADFLTRIRNAQQSGHRMVEIPASNVKKRMTEILYENG
                     YILKYKFEKPEGEFEKIMIALKYDPATRQPVIQKLQRASRPGLRKYSGAEDLPRVSSG
                     LGIAIVSTNKGIMTDKQARAQHVGGEVLCYIF"
     gene            27196..27753
                     /gene="rplF"
                     /locus_tag="PKM27_08650"
     CDS             27196..27753
                     /gene="rplF"
                     /locus_tag="PKM27_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNR07371.1"
                     /translation="MSRIGKLPISLPKGVEVKIDKNRVTVKGPKGSLVQEVDYDMKVT
                     VEDGTLVVSRPSDQKRHKALHGTTRAILANIIKGVSEGFNQDMEIVGVGYRTSNTGNL
                     LEIGVGHSHPIMFYVPDELKVETLTEKGKAPLIKLSGTDRQLLGQISAKLRTFRKPEP
                     YKGKGIKYAGEILRRKAGKAAGKAK"
     gene            27791..28144
                     /gene="rplR"
                     /locus_tag="PKM27_08655"
     CDS             27791..28144
                     /gene="rplR"
                     /locus_tag="PKM27_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNR07372.1"
                     /translation="MKSTKNYGKQKIRYRIRKKVSGSAVRPRLSVFRSNKSIYCQLID
                     DATGHTLASTSSHDETVPKEVNKTEQAKAVGNLLASKAKGLGIENVVFDRGGNLYHGR
                     IKALADGAREGGLKF"
     gene            28173..>28515
                     /locus_tag="PKM27_08660"
     CDS             28173..>28515
                     /locus_tag="PKM27_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNR07373.1"
                     /translation="MANRVVKRVKPSEAELKEKMVALNRVAKVTKGGRTFSFAAIVVV
                     GDGNGVVGHGLGKARDVQESIAKAVDDAKKNLIRVNIHKGTIPHIQTGKYGAGRVLIK
                     PASEGTGVIAGG"
     assembly_gap    28516..28525
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28526..28750
                     /locus_tag="PKM27_08665"
     CDS             <28526..28750
                     /locus_tag="PKM27_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07374.1"
                     /translation="TGHRYNDVIGYVIAGGAMRAVLDMAGVHNVLGKSLGSSNPHNVI
                     KATLDALSKMRSPQEIAQQRKVALNKVFNG"
     gene            28762..28944
                     /gene="rpmD"
                     /locus_tag="PKM27_08670"
     CDS             28762..28944
                     /gene="rpmD"
                     /locus_tag="PKM27_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNR07375.1"
                     /translation="MTKIRITQTKSIIEKTDRQKKTIKALGLKGVRHSVEQQLTPQIE
                     GMLSKVAHLVSVDKIS"
     gene            28968..29414
                     /gene="rplO"
                     /locus_tag="PKM27_08675"
     CDS             28968..29414
                     /gene="rplO"
                     /locus_tag="PKM27_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNR07376.1"
                     /translation="MELNNLKPAKGATRNNKRRGRGIATGSGGTAGRGHKGHKARSGH
                     KAKRHFEGGQMPLQMRLPKIGFRNPAREEFAVLNLDQLQNYATKSGQNQIDLPWLIKA
                     GVLKPGQKIKILGRGELASGIQVTAHACSASAKKIIEEKGGTITLI"
     gene            29425..30789
                     /gene="secY"
                     /locus_tag="PKM27_08680"
     CDS             29425..30789
                     /gene="secY"
                     /locus_tag="PKM27_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNR07377.1"
                     /translation="MNSLIQTLKNIWKIEELRKRILFTLAVLAVFRFGSYIVLPGVVA
                     GQLQAAAGNRGANDLLNLVNIYTGGAFNNASIFALGIMPYITASIIVQLLGFALPYFQ
                     RLQQHEGESGRKKLNQITRILTVVITLLQGGTYLAYLNASNAIDPSVPKSVFYFVNSI
                     LLATGTVTAMWLGERITDKGIGNGVSLLIMIGIIARLPQAVIFEIQARFSSGGTFMLI
                     LELAALIVVVLGSILLVQGVRKIPIQFAKRMVGRTGLPVSGNRDYLPIKLNAAGVMPI
                     IFAQAIMFLPGFALQSLSGAAASSSGFVRSLNDFTSIPYNVIYFILVVLFTYVYVALI
                     VNSTQYSEYLKRQNAFIPGVKPGSPTAEFIDTITSKITLPGAVMLGLLAITPAMATAF
                     GINNAFAAFFGGSTILILVGVALDTLQQIESYLLMRKYDGLVKSGNIKGRTGSGMAPI
                     GAGS"
     gene            30811..31638
                     /gene="map"
                     /locus_tag="PKM27_08685"
     CDS             30811..31638
                     /gene="map"
                     /locus_tag="PKM27_08685"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNR07378.1"
                     /translation="MTKGKVVIKTEEEIELIRHSSRIACEAIAEVAKVIRPGYTGIEA
                     DKLADTYIKDHGGLPSFKGYGHPAFPASLCISINEAVVHGIPTHTPFREGDLVSLDCG
                     AYLNGFHGDVAYTLVLGEVPEAVMKLCVVTKRSLYLGIEQSRQGNRLGDIGFAIQQYV
                     EKENGFGVVRELVGHGVGKQLHEPPDVSNYGKRGNGLVLQENMVFAIEPMVNLGTRQI
                     RTLSDYWTVVSKDNKPSAHYEHTVAVRKTGGETLTDHTVIEEAIKNNKELSEISLKI"
     gene            31665..31883
                     /gene="infA"
                     /locus_tag="PKM27_08690"
     CDS             31665..31883
                     /gene="infA"
                     /locus_tag="PKM27_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNR07379.1"
                     /translation="MPKEDLIKQDGIIEEALSNAMFRVHLENDHVILATISGKMRMHY
                     IRLLPGDKVAVEMSPYDLSRGRIIYRYK"
     gene            31897..32013
                     /gene="ykgO"
                     /locus_tag="PKM27_08695"
     CDS             31897..32013
                     /gene="ykgO"
                     /locus_tag="PKM27_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNR07380.1"
                     /translation="MKVRTSIKKRSADCKIVRRKGKLYVINKKNPKFKQRQG"
     gene            32025..32402
                     /gene="rpsM"
                     /locus_tag="PKM27_08700"
     CDS             32025..32402
                     /gene="rpsM"
                     /locus_tag="PKM27_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNR07381.1"
                     /translation="MARISGIDLPKNKKGLIGLTYIFGIGTSSSAAILDKAGIDPDMK
                     VSAWTDEHVHKITKIIQDDYKVEGMLRSEVQLNIKRLMDIGCYRGIRHRRGLPVRGQN
                     TQTNARTRKGKRKTVANKKKVTK"
     gene            32422..32808
                     /gene="rpsK"
                     /locus_tag="PKM27_08705"
     CDS             32422..32808
                     /gene="rpsK"
                     /locus_tag="PKM27_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNR07382.1"
                     /translation="MSQVKKAKKRKVHVESEGIAYVLASFNNVIVTITNKQGQVVSWS
                     SSGREGFRGSKKNTPYAAQKTASHAATLAHEAGVRSADVYVKGPGTGREAAIRGINDV
                     GIKVTTITDTTPIPHNGCRPPKKRRV"
     gene            32862..33473
                     /gene="rpsD"
                     /locus_tag="PKM27_08710"
     CDS             32862..33473
                     /gene="rpsD"
                     /locus_tag="PKM27_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNR07383.1"
                     /translation="MARYTGPSTKIARTFGEPIFGQDKSFDRKKYAPGQHGQSRKKKQ
                     KSDYAVQLKEKQKAKHTYGILERQFRKTFELASRKHGVTGTLLLQLLESRLDNTVYRL
                     GIAPTRRAARQLVSHKHIAVDGKIVNIPSALLKPGDVVAVRGKSANLEHIQNQVHAKT
                     DIRKSPWLEWNPERMEGKFLIYPERDMIPERINEQLIVELYSK"
     gene            33521..34519
                     /locus_tag="PKM27_08715"
     CDS             33521..34519
                     /locus_tag="PKM27_08715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNR07384.1"
                     /translation="MSILNFQKPEKIILQKANDFDGVFEFKPLEPGFGQTIGNSLRRI
                     LLSSLEGHAISGIRIAGVDHEFSTIKGVVEDIVDIILNLKQIRLKKVNNTDDNSEDTI
                     YLTINRKDEFRAGEIEKFTNVYKVMNPDLLICRMEPFVNLEIELKITKGRGYVPADDT
                     YPKDAPIGLIPLDAIYTPIRNVAYSISNTRVGQRTDYEKLTIELKTDGTIHPEEAIKE
                     ASRIMIQHLMLITDENITFNDASRKEDAIVDEHILHMRKLLKTSLEDLDLSVRAYNCL
                     KAAKINTLGEMVKYDTHELLKFRNFGKKSLVEIEELLSTKGLSFGMDLSKYKLDEE"
     gene            34599..34946
                     /gene="rplQ"
                     /locus_tag="PKM27_08720"
                     /pseudo
     CDS             34599..34946
                     /gene="rplQ"
                     /locus_tag="PKM27_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            35285..36412
                     /gene="carA"
                     /locus_tag="PKM27_08725"
     CDS             35285..36412
                     /gene="carA"
                     /locus_tag="PKM27_08725"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNR07385.1"
                     /translation="MSEAYQSPAPAVLLLQDGKVFHGEAIGVKGTASGELCFNTGMTG
                     YQEIFTDPSYYGQLLIATNVHIGNYGTKATETESSSVKIAGFICKNFTTDFSRLQAES
                     SIQDYFVREKLVGIQQVDTRELVRHIRNKGAMNAVISSEIFDLGRLQEILGKTPNMDG
                     LELASRVSTKEEYFLGDSSASYKIAVLDFGTKRNILLNLVNRDCYLRIFPAETPAEKI
                     LAWGADGYFLSNGPGDPAAMDYAIESTRAILKTGKPLFGICLGHQILALALNVPTFKM
                     HHGHRGINHPVKNLVSGKCEITSQNHGFCVDPETLLQKTDLIEITHVNLNDDTVEGIR
                     LKGRPAFSVQYHPEASPGPHDAHYLFDQFIDELADYKMNKS"
     gene            36414..37700
                     /gene="eno"
                     /locus_tag="PKM27_08730"
     CDS             36414..37700
                     /gene="eno"
                     /locus_tag="PKM27_08730"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNR07386.1"
                     /translation="MSVIVDIRSREILDSRGNPTVEVELETEKGTLGRAAVPSGASTG
                     KHEAVELRDGNKDRYLGKGVLKACQNIEETIREELIGVEVHDQKYIDQLLIELDGTFN
                     KSKLGANATLGVSLAAAKAAALETNQSLYRYLGGVNAHVMPVPLMNILNGGAHADNKI
                     DFQEFMIIPIGADFFSEALRMGVEVFHNLKSVLKSKGYSTNVGDEGGFAPNLNSNEEA
                     IEIVLEAIQKAGYKPGEDIAIALDAAVSELWDEEKQRYVFKKSNKMEFDTPGMISFWK
                     EWCHKYPILSIEDGLHEDDWSGWSAMTKELGNKIQIVGDDFFVTNTDRLQKGIDSDAA
                     NSILIKVNQIGTLSETIDAVQLANRNAYTSIISHRSGETEDTFIADLAVALNTGQIKT
                     GSASRSDRVAKYNQLLRIEEELGESAVYPGKAFMII"
     gene            37740..38078
                     /locus_tag="PKM27_08735"
     CDS             37740..38078
                     /locus_tag="PKM27_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNR07387.1"
                     /translation="MLPIKAITASLRFHLSKILPDYLVNRYFITAMVFFIWMIFFDKN
                     KWVNQWQLSQSIYRLEQEQKILAGMIVEARRDKEDLEKNKEKYAREKYYMHKENEDIF
                     IFEENLNPDK"
     gene            38075..38947
                     /gene="sucD"
                     /locus_tag="PKM27_08740"
     CDS             38075..38947
                     /gene="sucD"
                     /locus_tag="PKM27_08740"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNR07388.1"
                     /translation="MSVLVNRDSRVIVQGFTGKEGTFHATQMIEYGTAVVGGVTPGKG
                     GQRHLDRPVFNNVEDAVKAAGANVSIIFVPPAFAADAIMESAAAGIGVIVCITEGIPV
                     QDMVRVKEYIRDFPCRLIGPNCPGIITPNEAKVGIMPGFIHQKGHIGIVSRSGTLTYE
                     AVDQVTKAGMGQSTCIGIGGDPIVGTTTLDAVQLLMQDPETHGIIMIGEIGGGMEAEA
                     AYWIKEHGTKPVVGFIAGQTAPKGRKMGHAGAIIGGAADTAEAKMKIMSECGIHVVKS
                     PAEIGESMRILMKQ"
BASE COUNT        10980 a         9689 c         9893 g         8484 t
ORIGIN      
        1 gttttacttg aatgagtatt aaaaacgtgg agcatcttcg atgctttgag aggtttcacc
       61 cgtttggacg aagcttagcg atttcgcaag gcaagccgca gataagagca aggatttaat
      121 tcccccggtt tattacggca tcggagatgc cagaccttac tggtaaaaaa taagaacaaa
      181 caatgacttc ggagaagccc aacgggtgca gtccgaatta aaaattcatt tttattttgt
      241 cgataaagga gatgatggtg gtcgtcattt gggtggtctt acgtgagacc ttgtgacatt
      301 gatgttcgaa gatgttgggc ttctccgaag ccggagtttt acttgaatga gtattaaaaa
      361 cgtggagcat cttcgatgct tgagaggttt cccacaagga ccctaagggt tgcccgctaa
      421 gacacagcga agcgaagggc acggtttgga cgaagattgg cttcggccgt cccccactcg
      481 gacttctgtc tcgaaacata taatgatatt gtttcgtaaa aatcgcgccg gaaacagaga
      541 tgcagaatgt ttggctggaa ggtttttaca aattttccta tttgtatttg aacctttcat
      601 cttgctaaaa ggcttcaggc gggacgcact gaagcaaccc gaacttgccc tgaaagggcc
      661 ctattcccgc agccttgggc gacacgccca aggctgcggg ccagatgctc acccgctctg
      721 aaaggactcc attcaatcgc ataaactcat tcaatcccaa ccgattgaac tctcccgcaa
      781 tggcatttta ttaacttcac tcctaaattt ccacttaaga tgaaagccat ttacgccctc
      841 ctggtaggca tagacagtta taatccgcct gttcccaaac tcagcggggc gaaaaaagac
      901 attgaaaaaa taaaaaccta cctcgaaaaa aacttcaatg accggcccct gcatatcaaa
      961 accctgctcg agcaggaagc gacctatgat caatttattc aaaatttcag ggaccacctg
     1021 ggccaggcca aagccgatga cgtggtctgg ttccattaca gcggccacgg gtccaggcaa
     1081 acctcagcgg aagaattcga aaaactaaat tctggaaaaa aggacgaaac cctggtccta
     1141 tacgacagca ggccttccgg aaaagacctg gccgataaag aactggccat actcattcat
     1201 gagatcggta cacgaggtcc ccatatcctg gtgaccctgg attgttgtca tagcggatcc
     1261 gggacgagga gcgctgaagc gttcacggaa agattgtctg ccgaccgcgg cgataaacga
     1321 actattgatt cctacctcaa tggatatttt aatcaaaggg ggaccacgct gccagaggcc
     1381 aggtatttat tttttgccgc ctgcaaccgg tttcagagcg ccaaagaatc gttcgatgga
     1441 ggcggtttat tcaccgagaa cctgatccag actctggaaa aaagcaaaaa agacatttct
     1501 tatgcagacc ttctcataag gctcaggcag ggagtggttt ccatgaaatg ggatcaggat
     1561 ccccagatgg aagtcgtggg cgatttgagc agctataccc aattcctcga tggatccgtg
     1621 gtaaaaggag cacccaaata tgcggtccgt cacgatgaag agggttgggc catggaggcc
     1681 gggcaaatct tgggaattac tgaggaggta aaactggcca tctatcccga ccagtccaat
     1741 gaacccctgg gtgcagctat cgtaaaccaa tcctcggctc agatatccag gatctccacc
     1801 gaatctgccc tggatacgga tgcaaaatat tgggctgttc cattgaatat tcccgggatc
     1861 ccttttaaaa ccggcttgaa tgtcgatgag cacttcaggg aggcatttgt tgaggaagcg
     1921 gaaaggtttt taaacgtccg ttttaatatt cccggtaatg agaatgaccc atataccctt
     1981 attcaaaaag agggttattt cgaattgata aaccatgaaa acggcctaac catcttaagg
     2041 gctgatataa aatttaaaca ggcaatccat gaattagtca gaaaactgga tcaaatagcc
     2101 acctggaccc ggtttttaag acttcaaaat aataagacgg ccctcaaccc ggattgctgc
     2161 agcctgtcgc tggatattga ggtctcgccg ggccggatgc aaaattttcc accgggggag
     2221 atcagactta agagcgatgg gtccaggatt ccctacaaat tgtggattca gaatcaattc
     2281 acccagcccc tgcattttgc cttgatttac ctttcggatc attacggttc cgtacccatt
     2341 aaaaatgaac cgctggagaa gtcggatcag cccactttgt tttggggagg caccgaaaat
     2401 gatgttttta tgctcccgga aggaagtgtt tcctccaaag acagctttct gatcatcatc
     2461 agtacggaac gaacggatga tttccagttt accctggaag aaatggaatt cggcgacttg
     2521 aaatttgatg acagggccat tcccgggatg aaccgaagca ctaaagtgaa aggggaatgg
     2581 ttcaccagcc gatttatggt tattttggaa aggtaatgat tgtcgcccag gctgttcatt
     2641 ttgccgaata aacatggatt ctgagaataa tatgaccgga aactaatcaa tcattggtat
     2701 gcctttcaag cccgtcctat ttcttgcctt tgccaatgat aaagtagacc agaccgccta
     2761 tctgagaaac ctgtccgctg aaatgcacgg catccgcaaa gccctgggtg aagccgtcag
     2821 cagtggcttg tgtgaagtgg tggaacggtc ctccgcttcc gtcggtgata ttctggacgt
     2881 cttccaggac ccggtctttg cttcgaggat atccctgttt cattatggag gtcatgccaa
     2941 tggattttcc cttttgttgg aatccgagga tggcactaag gccttggcgc ataaggaagg
     3001 cctggtcccc tttctgtcca ggcaggaaaa cctcaaactg gtattcttaa atggttgttc
     3061 ttcggaggga caggctgcgg atctgctcaa ggccggtatt tccgcggtga ttggcaccac
     3121 ttctgccata aatgatgagg tggccacctc gcttgcgatc cggttctaca gcagcctggg
     3181 ggacggttca gggatagaaa aggcatgggc ggacgcaaca gacgaagtca aaatatcaaa
     3241 ggggacgggc aatacccgtg ccttgttatg ggaagggaag aatgaggcat ccgagcattt
     3301 tccatgggtc atccggttca ggccgggcgc ggaaatcata aaagaatgga atctgcccga
     3361 tatatccggt aacccgctgt atgggcttcc ggacattcct ccatccttta atcttcccga
     3421 gtcccctttt ttgtatctca accggtatga acgaaggcac gcggaagtat tttttggaag
     3481 atcttattat atccggtcct tgttttcttc cgtccatgag gcccaggccc ctcctataat
     3541 ccttctctat ggccagtcgg gcgtaggtaa atcaagcttg ttggaagccg ggctgcaacc
     3601 caggcttgaa tccaggttcg agatcctcta cctgcgacgg tcgggagctc ttgggctcag
     3661 cgccacggtt tataatgcct tgctcgaaaa gtgcgggttt aaaccggaag tcagcaccat
     3721 ttcgatggcg acaaaggatt cagaccgggt cctggatgaa ctccggaaca ttgccgccaa
     3781 tattgaggag acctttaaac cggatgtcca gcagttgatt caaaaaatag ccggaaccaa
     3841 tgccacggat accggatcga caaccggcgg ggaaacgccg ccatcgaccc tgtcggaagc
     3901 ctggaaacag gcagaggcta aattaaataa acctttggtc atcatcctcg atcagctgga
     3961 agaagcatac acccggccaa atgagcgatt gggcaatgag atgaatcaat ggtttatcca
     4021 tctcaaagcc atttttggaa atcccgcttt gatgccccag ggaaagatta ttctgagttt
     4081 ccgaaaggag tatcaccccg aaatcgaaga atactgcaaa aactacgaac tgccaaggag
     4141 caaaatcttt ttggaacaca tcaccaaaag ggatgtattg gacgtattcc gtggattcat
     4201 ggaaacccca aggttaaagt ccaggtacaa cctgcaggtt gaggatggct tacccgaaat
     4261 cattgccgca gatcttgcag ccgataagga ttctcccatc gcgcccatgt tgcagatcct
     4321 cctcactaag ttgtggatca aatcattgac ctccaacccg gaagcgccgg cctttacaca
     4381 cgccctttac cgtgatctga aagaagaggg ccttgcaatg gatgagttcc tgcaaaacca
     4441 gctcaacgcc ctcaaaaaga aatttcccga actccatcat aatgggtttg ccctggaggt
     4501 gctcgggttt tattgcactc ccaacggaac ctcagcggca cggacggagc aacagttggt
     4561 tgaaaattac cccgatgcgg ttccggaaat cagaaatgtc ctggccggct gcatggaact
     4621 ttttctgatt accgacctgg gaggcgtcga acgcagcgcc atgcttgcac acgacaccct
     4681 tgcgaaatcg gtcgtaaaac tgcaacaacg gtctgcccgt cctttgcaac aagccagaag
     4741 ggtgctccat tccagaatgg aggccgtccg ggggggatcc gcgtcttcaa ccctggatca
     4801 ttgggatttg aaactcatag acggggtgct taaatattta cctgcagttt cgggggaaga
     4861 gaaaaatctg atcgaagcca gccgtaaagc ggttttgaaa aaagaaaaag aaaaggtttt
     4921 acttaaatac agcaaaaggg gcctggcggc gattacgggg attggggccc tgttgatcgg
     4981 attattgtat tttcagtcca gggcgcacgc caaggcttcc tttgtcaatc atatggctgc
     5041 cgcttctgcc attaatctca ggaaggaccc caccctgtct ttgatgcagg ccgcagaagg
     5101 aatgaaagta aatcaggaaa actccattgc tgaaatcaag aaatcattga ccgatgcttt
     5161 ttacagtgcg gtccaaaacc ggactccctg gtatgacacg gtattttcta cggacctgaa
     5221 ttactatgat ctggttttcc acgaaaacga aaagaccatg attcctttta cggagacgga
     5281 ttcgcttgaa attttggact acgacgggaa cttgcttggc gctctggtca ccgatccgtt
     5341 atccccggat gatccggcga tggctggagc gcccgaagac gcgtttgcct attttacagg
     5401 cttgaaatgg aaccgggacg ggcattccat cgttgcattg aacagtgatg gcatcctgca
     5461 attccatgaa gcatcgggga gcttgaaaaa aaggattgga atggcccatg agtatcaggc
     5521 cttcgatgta tcgccggttt tgaaccaggc catcgcttat gccatcggag acagccaggg
     5581 atatgcacaa gaccagatcc gtttgttcga tatggatgga aacctgctca aatcctttgg
     5641 caccaccgca gatattaaag acattcggtt caccgccgat ggtaaaaacg tcctactgac
     5701 ccggttcgac acccttctta cagcaataga tcaggcggga ctgccccaga accctgaacc
     5761 ggattcgtgg aaaaaagtgg aaattttaaa cttggatgga atgcggatca ggcaactcga
     5821 tgccaaggag ggcgaagtcc ttgacctttg gacttctcct tcaggtaagt ggttggccac
     5881 ggtgcacgcc ggggggacct tggttctatg gacctcggaa ggaaggcggt ttaatacctt
     5941 caagcccccg gtcctgcccg gtcgatcggg gatgcccgga accagggaaa atgaaatact
     6001 tcatattgat tttcacccca atgacagtct gatggccatt tgttttcgcc aggatgcagc
     6061 ctgtctttac cccatatacg ggaaatccgc tgcccccgtc atgttatggc acgagcacct
     6121 ggtcaccttt gcccgctttt cggaagatgg caggcgcgtg ctgacgggtt cggctgacaa
     6181 tacagccgcg gtttgggatt tgaagggaaa tcaattgtat cacctgcttg gccataaaaa
     6241 cgatgtgacc ggaggtaagt ttattgatca gggcgaacag gtattgacca cttcgctaga
     6301 tggcactgtg cgccgatggg tattggaaaa ttacccggat cttcagttta ccgggcaccg
     6361 gggggcggtc acttatatcg atttggatcc gttggaccaa tacctggtct ctgccggagt
     6421 ggatcatacg atgagagtat gggatataaa aagcaaaaga gaagtggatc ggctggacct
     6481 gggtgatcag ggggtaaggt atgccatttt caccggtgaa aatgaaatcc tgggaatggg
     6541 cgtcaaaaat caggcgtttt tatacaaact ctttgaagcg acgccggtga aattcatagg
     6601 gcataccgga ccggtggaat gggccggtgc aatcggggac cacatcgtca ccgcctcaaa
     6661 ggatggaagc atccggtatt ggaataagac cggaaaggaa gttaaacgaa tagagcccgg
     6721 acagggtgaa atgctgagcc tgatcgtttc tcagcaggac agtatcgtgg ccgcaggaag
     6781 tgaaagcggc accatattct tgtttgacga aaaaggagaa gtcattgccg aattggaagg
     6841 acaccgggat gccgtcattt atctgaatgt atcggatgat ggaagccgcc tggtatccac
     6901 ttcccgggac ggaacggcca tcatctggga tttgaaatca aaatcggaag tcgcccggct
     6961 ggaccgcatt gcctgcgccc cttaccagga ttgccaggtc aattccgcca gtttctcttc
     7021 tgatggttcc aggattgtga ccacttcttc agaccggaca atccggcttt gggattccga
     7081 tgggaacctg aaggccagca tggtaggcca taccggtgaa gtggtcgatg ccttttttac
     7141 tccaaacgat gatatgattt attccttttc agcggatcaa accttgcgcc tttgggattt
     7201 ccagggtaaa gaaatcattg cctaccgcgg gcataccggg aagatcaatt gtgcttatat
     7261 ggattttgat atgaaccaga tttttacagc ctccgaggac ggcaccattc gcgcctggct
     7321 tagcccaatc ggcgtttacc ggtggatgag ggattcgaaa cagtttaaac ctatatttca
     7381 tggaaaatac aatcaatagc tggaatggct tgaatctgga cgaccttcgc caggtcatgg
     7441 accccgatgc ggatgcggcg atctcgtcac tgtataaatc gagggctttt aattcccttc
     7501 gcgatatgct cagggatatg gccaggaatg acaattttgt tcccgattcc atgccggcgg
     7561 gcattcgcga ttttcttaca gcagaactgc aattcgtgcc ttccgcagaa gatattgcag
     7621 cttttgaaag ggcgcaccgg gtctggaaag aaaacggcgt tcaattcata tttatactgt
     7681 tctttcggtc tttaccctat acttatatgg ccgaaaaacc cgccaatgta ttgcggatga
     7741 ccaaaattct cgaggcgcat ccgcaacgca ggatatttga gaccgcccag ttcgtatttg
     7801 acgtcagtga caaagcatgg tggacgcctg aaaagagggg gctgatcacc gcactgaaga
     7861 tccggttgat gcacgcggcc atgaggtaca acctcctgta taatccggaa gaagaaaact
     7921 ggaataagga agcctggggc atgccgatca gccaggagga cctgatcgct accaaccagg
     7981 tattttctct ggagtttttc aagggcatgg ctatccttgg gcaagccctg gatgaggagg
     8041 accaaaacgc ctggttccat acctggaaaa caattggaaa aatcatgggc atccaggaca
     8101 acttattatg caagaccaaa gaggaagcgt ggaccctgca aaaggccatg tacaaccact
     8161 tgtttccgga tgataaccgg gccggcatcg ccctggcgaa agccctggtg gaaaccctgg
     8221 ccacctttct gctcagtaaa aaattgatcc tgctcatgat gaaaaaaatg atcctggatg
     8281 aagatcaccc cgatctgttt taccgggtgc tcgggcccag ctttgccaaa gaataccctt
     8341 ccgtgtttaa aaaatcaagg tccgtagaag aagcggaaga ggatgccgcg gctgaacatc
     8401 aggaatttta cgacgaattg atgacctata aagaaaaagt cagatcctac cgggagaatc
     8461 aaaaacaaaa agatccggtc agcagagggg aaggcaataa aaacctggtg gatcttcagc
     8521 tcgatgtctt tgaagaactg gcggaaaaaa tggatccaaa ggggccggtg gaccggggca
     8581 tcgtgcatga tttgtttaaa acggccatga gctccgtcgg cggggtgatc atcggcatcc
     8641 tgtccctgta cttcagggag gggaaagaag ccggctttcg tatccccacc gacttaaaag
     8701 aatattgggc tttatcatga tttcaggaaa aagcctgttt taatttccga tacaaacatg
     8761 accgatttac aaagaagcat tattcgctat tatgatgcca ccctgttgga ttaccgggcc
     8821 ttttgggtgg gcaaacggga tttggctttt cacttcggat attacgatac cagcaaggag
     8881 tcccacaggc aatccctgga aaatatgaac agggtcatgg ccggcaaggc cggcatggat
     8941 gcttccgacc ttgtcctgga tgcgggctgc ggccagggag ggagtgcgct ttggctcgca
     9001 acccatattg gatgcgtcat ccaggggatt tccctggtgc cccatcaggt tgaaatggcc
     9061 aaccgggtgg caaaagccag gggcctggaa aataaagcac gtttttatat tcaggattat
     9121 gccaaaaccg gatttccgga ggagagcttt accgtcattt gggcctgtga aagtttatgc
     9181 catgctcccg agaaaaaagc tttttaccag gaagccttca ggttactgaa gccgggaggc
     9241 aggttggtgg tagccgaata catcaggaag ggccggaaca acgcggtgca ggatgaaaac
     9301 attctgcaaa actggttttc gggatggtcg atgccggatc tcgatacctg gcaggaacat
     9361 tgggatcata tgagggaggc tggatttacc cggatccagc atgaagacat caccggtcac
     9421 gtatcccctt ccttgcaccg cttgttcaaa atgtccaaac ggctgctggg tcttggcaaa
     9481 tttttacacc tggtccggat caggaaccat atccggcacc ggaatcaagt ggcttcgatc
     9541 agccagtacg tggcgcttac aaaaaacctt tggaattatt atatttacac agcctccaaa
     9601 cccgggcttc caaaatgaca gaacatggct tatatattcc ggatggttat gttttgctgt
     9661 cgggtatata ccgggacgat gttcatgaaa tcctcaaggc caaaagaaaa tccgaccaaa
     9721 gggaggtcgt tttaaaggtc agcaggcctt ccaacaacga cattcttaaa atttccaaac
     9781 tcagccatga ataccatacc ttaaaaaacc tggaccatga gggcgtcatc cgcgttttgg
     9841 gactgcattc aaaaaataaa tccgtctgca tcgtcgaaga attttttgaa ggggaaacgc
     9901 ttaaatcaag aatcttcaat caggcattta ccgtcccggc gttttttcag gtggcgcttc
     9961 aactgacgga tatcctggcc tatgttcata gccagggggt cattcacaag gatatcaatt
    10021 cctccaatat cctcatttca aaagacaata aggtcaagct gattgatttt ggcatcagca
    10081 cccatattat gaatgaagaa caggatatgg tgattcccga cctgatcgaa ggcacgctga
    10141 cctacatttc cccggaacaa accgggcgaa ccagttattc catttcggcc ggcagtgacc
    10201 tctattccct ggggatcgtt ttttacgaga tgctgagtgg taaaccgccg ttcgattccg
    10261 ccgatgccct cgaggtcatt cacttccacc tttcccgtac acctgccccc ctgggcaagg
    10321 tattgcccag gttgccttcc ggcctggaga aaatcattgc cgaattgctg gaaaaaaacc
    10381 cggatgaccg gtatcaaagc gccggcggtt tgcatcacga tttaaaattg ctgcagggcg
    10441 agattgaatc cggcaaggct tcctcttttt tcaaaacaag gaccaacgac aaaggagggc
    10501 ggttccggaa ggcccagcgg ttgtatggca gggagcagga ggtcgccagc atcatatcct
    10561 gttatgaaaa cctgcatcaa acacgcacca tgctggtcct ggtttcaggg tattcgggag
    10621 tgggaaagtc agccgtggtc aaacagttgc agcgcccggt gacggataaa aacgggctgt
    10681 ttatttccgg caagtttgat caattcaaaa gaaatattcc ttatttcgcc ttcattgaag
    10741 cctttgataa tctgctgcgc ggcctgcttg cggaaagcga cgataaaata aaagcctgga
    10801 aggaaaaaat ctgggaaaaa ttaggcgcta atgcctccct gatcagcgag gtcattccca
    10861 gcctggaatt catcacgggc aaactgccgc tcccggaaaa acttcccccg gcagaacagg
    10921 aattcaggtt caggttggtt ttgctggact ttatctattg tttcagcact acggacaggc
    10981 cgctggttat ttttttggat gatttgcaat gggctgacct cccttcgctg aatctcattg
    11041 aacggatcct ctccctccgc cggtcggaga atctcctgat cgtgggcgcc taccggagca
    11101 atgaagtcaa tgaattgcac cccttgtcga tgaccattca tcagataaaa ggcgaacagg
    11161 tcaaggtcat ggatatccgg cttcagcctt tggatcaaaa cacgaccatt caaattgttg
    11221 cggattcatt cggaatgcct gaggcccagg caacggatct tggcctgcat gtttacgcca
    11281 aaaccaaagg caatccgttt tttatcaatc gttttttgca atccctttac gataataaat
    11341 atttgacgct ggatgcctcc cggaaatgga cctgggaccg gaataaaata gaaagccttt
    11401 cttacacaga caacgtcatc gatctgatgg ccaatgaaat gacttccttg cccgaaacga
    11461 gcagggaggc gatgaaatat gcggcggtgc ttggaaatac ctttaacctg agcaccttgt
    11521 cgatggtcat ggaaaaaaac caaaaccagg tatacccgtc tttgcttcct gcgctaaagg
    11581 gagggtatat cctgcccctt gaccagaatt acagggcatt gtccttatac acggaaggga
    11641 tcaacgagga atttgaaacg gaatcagaaa atcttttcat caatttcaga tttttgcacg
    11701 accgggtcca gcagtccgct tatggcctga ttgctcccgg ggaccgggaa accatacatt
    11761 tccggaccgc gaatcttttg tattcgaagt ccacccctga aaagctgaac gattttttat
    11821 ttgatatagc caaccatttc agcctgagta aaaatcgggt atttgagcaa ggcgaccgca
    11881 gatccattgc cgcgctgtat ctgaacgcgg ggtcaaaggc aaaagattcc acttcgtacg
    11941 atgtggccat caagtatctg tccgccgccc gggatttatt accggagcaa agctggcagt
    12001 cggattatga attgaccttt gccgtttatt ccgcactggg agaaagcgag tacctgaaca
    12061 gcaaccatga gaaagcggag tatctgtttg aacagatcct caaatatgcc cgaaccaacc
    12121 ttgaaaaatt aaaaatatat tacgttcatt cggcgcttta cctcaaaatc ggcaatacca
    12181 aaaagtccct ggaactggga cgggaggcga tgaaattata caacattcat tttcccgaaa
    12241 gtgaatgggc gattaaatca aaggcgacct gggaactggc caagtacctg ttcttatttt
    12301 ctacccgtta ccggaatgtg gagaaactct atcatctcaa ggattgtacg gaccaggaaa
    12361 tcattgccat aaatcagttc ctgatcgaca tcgccaccag tgcctaccag gaaaatcaga
    12421 acctgatggt gatcatcgtg ttgcgaatca tcaggcatta tctgaaatat ggatttaccg
    12481 acgcttccgg gtgggggttt tccggatttt cggtcattgc ttattcgggc atcgggcttt
    12541 cgaagcgggg cctgggcctg tggagcatta ccaggaaact tcatcagcgc accaggtccg
    12601 ccatcgtcag gaccaagctg gattataccg taaatgcttt ctacacccag tggaccaaac
    12661 cgctgaccga aaatctggat gagatcataa ataatgtgaa ggcctgcctg atgaacggcg
    12721 accccaattt tgcaggatac agcattgcct tgcagttttg gaaaaaatcg gcttcgggcc
    12781 tgaagctgac cgaggtcctg gacagtaccg gtgaatacct ggaatactta aaaaacaata
    12841 aaaatgtaac ggggctcaat tacatcatcc cctcggtcca ggccgtgaag gcattaatag
    12901 gcaaaacccc ggtcaacggc gattggaaca gcgaaggata cgacgaacca gcctttgcac
    12961 aacaattgaa tgcggtaggc aataaaacca atattgcatt cttttacaat tttaaattgc
    13021 ccctgtatta ttattttgac aagtatgaag aggggcttca atgggctgaa aagggagatg
    13081 ccctgcaggc ctatatcctg ggacattatt ttttatcgga atggtatttt tattacaatt
    13141 tgctgatcgc ctccctgtat gaaaagtttt ccacggcgga taaaagcaaa tatctgaaaa
    13201 tattccggcg caacagcaag tggttcaggc actggatcaa aggttgtaca gagaattttg
    13261 aacaacagct ttatattctg aaggcggaag aaatggtgat ggagggccag gtggaaaatt
    13321 ccatccggtg gtacgaaaaa gcaatagaag cggccttaag tcacggattc acccagtttg
    13381 ccgccatcgc caatgaacgg gcggctaaac acctggcggg aaagggaatc ctccggcaaa
    13441 gtgcgtttta cctcaaagcc gcttatgaat ggtacgaaga ttggaatgcc tttggcaaat
    13501 gcaggcagct gaaaggggca aggccggatc tgtttgggga caaaatcata caaatcaccg
    13561 gcaataccag gaccaattta ccgactgccg cactcgatta cggttcactc attaaagctt
    13621 ccaacagcat ttcgagcaaa ataaaactgg atgagctaat tcagcggttg atgaatgtat
    13681 tgctcgaaaa cgcgggggcc gaccgggggg tgctgatgat tcccaggcag ggcgaacttt
    13741 ttatcatcgc ggaaggaaag tccggcccgg ataatattca gggcaatatc caggtcccgg
    13801 tgaaaggcag caataaaatt ccacaatccc tgatcaatta ttgctggaat acgcaggaaa
    13861 caaaagcctt gtccaatgcg ctgagggacg aagcctattc caatgaccct tatgtgatgg
    13921 aacaccaggt ccggtccatg atcggcatac cgctttcgaa taaaggaaat aaagtgggat
    13981 tgatctatct cgagaataaa ctgctggaag gcgtttttac atccaacaaa ctggaggttt
    14041 tgaatatgtt gtccggccag atcggcattt cgattgacaa cgccctcctt tatgaaaccc
    14101 tggaatccaa agttacggag cgcacccggg accttgaaaa acagaaacag atcgccgaat
    14161 cccaaaccag ggaagccatc cagcagaaaa tgcttgccga cgaagaaagg aaaaagtcgg
    14221 atgccctcct gctcaatatc ttacccgatg aaattgccca ggaacttaaa cttaagggag
    14281 aatcccaggc caggcaatac cattccgtca ccgtgatttt tacagacttc gtaaacttta
    14341 ctaccgtatg tgaatatttg cctccggccg aactggtggc cgagctcaac aaatgttttg
    14401 ccgcctttga taaaattgtg gatggccatg gattggaaaa aattaaaacc atcggggatg
    14461 cctacctggc tgccagtggc cttccccgga taaacccgga ccatgccacc cacgcctgcc
    14521 gggcagccat ggatatcctg aattgggtca gggcaccgga aaacaagtgt ctttttaata
    14581 taaggatcgg gattaacagc ggtcccgtga ttgcgggcat cgtaggcatg aaaaaatatg
    14641 tttatgatat ctggggagat accgtaaata cggcggcccg gatggaatct tcatccagtc
    14701 cgggcaaaat aaatatatcg gaatcgacct atcagctggt gaaggaaaag tttaactgcg
    14761 tctgccgggg taaaatcgag gccaaaaaca aagggagcat caatatgtac ttcctggagg
    14821 agaacccttt acccggtgtg cttactactg ccgtcggctg agccggagta ttaatccctt
    14881 gacttgaacc attgtatttt cggaaacaaa atcccagatg gttttttata tgcttcccag
    14941 ttctggtgct tcgagaggga ggatcatatt caccaaaaaa tagcccgtcc atacccaggc
    15001 caggatcaac cagcccaacc attgcagtcc taattaaaaa ttattatatt ttttttgata
    15061 aaggagatcg gggtgaccgt ggtgaccatt gctcttaccc gcaccttctc attgccctga
    15121 aagggcccta tccccgcagc cttgggcgac cagcccaagg ctgcggggat agggctcaac
    15181 cgccctgaaa gggcaccata aagtgcctga ttatttatgg cgggacttaa aaccggggat
    15241 tgtcggaaac aaaatccctg attgtttttt ataggcctcc caaccttcgt gacgggatag
    15301 agaacggtct ttcaggatca tattcaccaa aaaatagccc gaccataccc aggccaatac
    15361 cgcccatccc agccaatggc ttgccatgag ggcgtaggtt gcataaatca gcatttctcc
    15421 caggtaattc gggttgcgcg tgcggcgaaa tatgccttcc gtgatgaggc ctggattgta
    15481 tttgaggctg taatacttct gggcgtcgct ggtcatcatg attaccacgc ccatcgtatg
    15541 gataaaaata caaaggccca caaaccagga agccaaagcg aacccctcct catgaaacca
    15601 gggactgatc aggatcaggg gaaacaacca gtacaaaccc aaaaccaggg caaaggacat
    15661 gaaggccccg cccagggtga ttttgatatc ccagcgccgg tcggggaaat acctgtcctt
    15721 gatcagccaa caaataccat aggagccatg cagccctgca taaacccagg cttcggccga
    15781 gtaattgtcg aaccaataca tcagaaacaa tactaaaggc agggtgcctc ctttctgaaa
    15841 gttgatcagg gccgaaagtt tgatcagcct gggcccgccc aggaaatcct ggctcaggta
    15901 attttgcagt cttgtcagtt tttccagcat tcaaaaaagg tggttggtgg gtagtgggta
    15961 gtcacgccaa acaccaccca ccatccacca cccaccattc accctacaaa gatcttccca
    16021 ttccaccatt ccttttcaat aatggcctgg tatttaccat agaagttgag ggcgggttcg
    16081 ttccattcca atacctgcca tttgaccagc ctgcaatcca ggttgacggc ttcctggagc
    16141 agagcgtcaa agaggagccg gccggtcccc agtccgcgga gggattcttt aaccacaaaa
    16201 tcttcgaggt agagcatcct gcctttccag gtgctgaaac agacataata tatagcggta
    16261 cccacaatgg ccccttccct gtccaccgcc acgagggcgt caaagacccc ttccaggtaa
    16321 tttttttcgt agtcctggag ggtggcggtt accgcctccg gcgctttttc atagacagcc
    16381 agttcccgga ccaaatcgag cagggaagcc aggtcttcta ggctggcttt ccgcacaaaa
    16441 gttgaagaat tcatcttgat ttttcttgtt tgtgttgtcg aaaataatca tatctttgca
    16501 gacgcttttt aaaataaagg ccgattttaa ataaaaatca ttgataatca gtaagtaata
    16561 aatgaaatca ttatatgcca accattaatc agctggttcg ttcgggtaga aaacaggtta
    16621 aagccaaaag taaatcccgt gcgctggatg cctgtcctca aaaaagaggg gtatgtaccc
    16681 gtgtatatac cactacccct aaaaaaccga attccgcgct ccggaaggtg gccaaagtcc
    16741 ggatgaccaa tggcattgaa gtgatcgctt acattccggg cgaagggcac aacctgcagg
    16801 agcactccat ggtactggtt cgcgggggca ggatcaagga ccttccggga gtaaggtatt
    16861 ccatcgttcg cggggccatc gataccgccg gggtaaacaa ccgtaaaaaa tcaaggtcga
    16921 ggtacgggac caaaagacct aaaaaataat cattcaacgt cttaatcttc cagttaccga
    16981 tgaggaaaag aaaaccaaaa gcaagagcca tccagggaga ccccaggtac aacgacaccc
    17041 tggtcagccg gtttataaac aacctccagt ggagcggcaa aaaatcaggc gcccagtcca
    17101 tcttttatga tgccatggat attgtaaaag caaaaaatgc cgaagagaac gaactggaga
    17161 tctggcggaa ggctttggac aacatcatgc cccatgtgga agtgcgcagc cgccggatcg
    17221 ggggagctac tttccagatc ccgaccgaac tcccggcaaa ccgcaaggta ttttttggca
    17281 tgcaatggat gatccgttat gcccgcgccc gtgccgggaa aggcatggct gaaaaactgg
    17341 ccgctgaaat catcgctgcc cagaaagggg aaggggctgc cgttaagaaa aaagaagata
    17401 tccataaaat ggccgaagcc aaccgggctt tcgccagctt cagataaata aaatccatca
    17461 acaaccatcc aggctcatgg cagcaaaaga tttaagatat acgcgaaaca ttggcatcgc
    17521 cgcccatatc gatgccggga agacgaccac cacggagcgt atactttatt acacaggcat
    17581 cacccacaag ctgggtgagg tgcatgacgg cgctgcgacc atggaccata tggaacagga
    17641 gcaggagcgc ggcatcacca ttaccgccgc agccaccaaa accgcctgga actggaatgg
    17701 agatgaatac accatcaata tcatagatac ccccgggcac gtggatttca ccgtagaggt
    17761 cgaacgttcc ctgagggtac tggatggcac cgtcgctttg ttttgcgcgg tttccggcgt
    17821 agaaccgcag tctgaaaccg tctggcgcca ggccgaccgc tataaagttc cccgcatcgg
    17881 ttttgtaaat aaaatggacc ggtcaggggc ggattttttt aatgtcgtcg atgaggtccg
    17941 taccaagctg ggctcccgtc cggtgcccct gcaggtaccg atcggctccg aacacaattt
    18001 taagggagtg gtggacctga tcaccaataa agccatggtc tggaacgaaa gcgaccaggg
    18061 catgacatat acggagatcc ccatcccgga ggacctggcc ggaaccgtgg ccacctggag
    18121 ggaaaagatg atggaagcgg ctgccgaata cgacgacgac atcatgcaga aatatttcga
    18181 tgacccttct tccatttcgg tgaaagaact gcaggctgcc ctgcgcaaag cggtcatcga
    18241 tatgaagatc gtgccgatga tgtgcggttc tgcttttaaa aacaagggag ttcaggctat
    18301 gctcgacgcc gtctgcacct tccttccttc ccctgccgat gtggatgcgg tggaagggat
    18361 caatccgaaa aatgaccaga aagaactgag aaaaccgctc aattcggaac cctttgcagc
    18421 cctggcattc aaaatcgtca acgacccctt cgtagggcgc ttgtcctata tgagggttta
    18481 ttcaggacgt ctcgaaaacg ggtcctatat cctgaatacc cgctccgata aaaaggaaag
    18541 gatttcccgc ctgtaccaga tgcatgccaa caaacaaaac cccatcgaat ccgtcgaagc
    18601 cggggatatt gcagccgcag tcgggatgaa agatatccgt accggagata ccctctgtga
    18661 tccggacaaa ccgatcgttc tggaaagcat gaaattcccc gagcccgtca ttggattggc
    18721 cgtggaaccc aagacccaga aagacctgga taaaatgggc cttgccctga ataaactggc
    18781 cgaagaagat cctaccttca aagtgcgttt tgacgaagac accaaccaaa ccatcatcag
    18841 cggcatgggt gaactccacc tggagatcat cgtcgaccgg atgagaaggg aatttggcgt
    18901 ggaatgcaac cagggcgccc cgcaggtcaa ctacaaggaa gcgctgaccc gcacggtaag
    18961 ccaccgtgaa aaactgaaaa aacaaaccgg gggttcgggt ctgttcgcgg acattgaatt
    19021 tgaaatcgga cccgcagatg aggaattcct gcagagcgaa gattttaagt ccggcaagac
    19081 caagatgcag tttgtgaacg atatcgtggg aggttctatt ccccgggaat tgatcccccc
    19141 gacccagaaa ggtttccttt ccatgatgga aaacggagtc ttggccggat atgccatcga
    19201 cagcatgaag atcaggatct ttgacggttc cacccacacg gtggactcca aaccggtcgc
    19261 cttcgagttg tgcgccaagg aaggtttccg ggcggcggcc cccaaagcgg gaccggtcat
    19321 tctcgaaccg atcatgaaac tggaagtggt caccccggaa gaatttgtcg gtcccgtgat
    19381 cggcgacctc aaccggagaa ggggcatgcc caaaggccag gaattgcggc ccggatctgc
    19441 agtggccatt caggccgatg taccgctttc cgagatgttt ggatatgtaa cccagttgcg
    19501 gaccatcacc tccggccggg cgagttcgac catggaattt tcgcattacg cccaggtgcc
    19561 ttccggcatt gcgacggatg tcattgacaa agcaaaagga gccgtaaagg cttgaacata
    19621 aggatatatt attaaatcat tgaaataggg aaatgaatca aaaaattaga atcaaactcc
    19681 gctcttatga ccacaacctg gtcgataagt cgacggagaa gatcgtaaag acggtccgct
    19741 ccagcggcgc tgtagttacc ggtccgattc cgctgccctc cgagagagaa gtttataccg
    19801 tgttgcgctc gccacacgtc aataaaaaag ctcgggagca gttccaactt cggacccata
    19861 agcgcctcat cgaaatttac accccgacgc aaaagacggt ggacgcgcta tccaaattag
    19921 agctcccgtc tggtgttgat atccaggtga aactcacctg atagcagggt ctttaattga
    19981 attaattttt ttgccatgag gatgcaaggg cccattgggt gcgggcgtcc ggcaggcaga
    20041 tattctgttt aaaaattaaa aacactgaaa agtgaacgga ttaattggaa aaaaggtagg
    20101 catgaccagc atctatgacg atgcgggaaa atacattgcc tgtacggttg tggaagccgg
    20161 tccaaacgtg gtcacccagg tcaaaacgga tgatacggat ggctattttg ccctgcagct
    20221 cggatttggg gaagcaaaac ccaagaatac cagccagcag atgaaagggc attttgagaa
    20281 ggtcaaaacc actcccaaac aaaaactggt ggagttcagg gattttcccc tcgccaaagc
    20341 ggccggggac aaggtcaccg tcgacgaggt ttttaagccg ggggataccg ttcatgcagt
    20401 gggcacttcc aagggaaaag gattccaggg cgtcgtgaaa aggcatggtt tcgggggcgt
    20461 tggtgaattg acccacggcc agcacgaccg gcaaagggct cccggttccg taggttcttc
    20521 ctcttatcca agcaaggtgt tcaaaggtct gagaatggcc ggtcgcaccg ggggcgagcg
    20581 ggtgaaagtg agaaatctga aagtcgtaaa aatatttccg gatcagaatt tattggtcat
    20641 tcgcggagca attcccggac acaatggttc ttatgtaatt attgaaaaaa agtaagccat
    20701 gaaagtatct gtatttaata cccagggagc ggacaccggc aggcaggtcg aattgcccga
    20761 agccattttc gggatcgagc ccaacaaaca cgcggtatgg cttgacgtga aggcctacct
    20821 ggccaatcag cgccagggta cggcggagac caaggaaaga aacctgattt ccggctccac
    20881 ccgcaagctg cacaagcaga aaggtaccgg gggatcccgg aaaggggaca tcaaaaaccc
    20941 ggttttccgc ggaggaggca ccattttcgg ccccaatccc agggattaca gcgaaaaagt
    21001 aaacaaaaag gtaaaaaccc tggcccgcaa atcggtcctt tccgacaagg ccgcccaggg
    21061 caaagtcatg gtgatcgaag acttttcgtt cgacaaaccg cggaccaaag agctgtcagc
    21121 cgttttgaaa aacctgaagg tgctggataa aaaagccctt tttgtaattc ccgattacga
    21181 ccagtccctc atcctttcgg gccgcaacct gcccaaaacc aggctgctgc atgcccggga
    21241 cctcaatacc tattccatcc tacactccaa tgtggtggtg gtcgccgaga gcgccctgcc
    21301 caaactggaa gaaacccttt tatccaccgc caaataagct gacctatgga aaaaaagatc
    21361 ttaatccggc ccatcatctc ggagaaagct gaaaaactca gcagcgccca acccaggtat
    21421 accttcctgg tcaacaaaga cgccaataaa atagaaatcc agaaagcggt acagcagcat
    21481 tacggcgtta ccgtgaaaga cgtaaatacc ttgaccaatc ccggaaagaa aaagtccagg
    21541 tctaccaaaa gaagggtaag cgtcggcatt aaaccttctt acaaaaaagc ggtcgtcacg
    21601 ctgaatgaag gggacagtat caatatctat ggtgaaattt aatttaaaca gggtaagtca
    21661 tgccagtaag aaaatttaaa ccagtcaccc cgggaacccg tttccggatc gccaatacct
    21721 atacggaaat caccaaggat gtaccggaaa agagcctggt gaaggcgctg accgatgttg
    21781 cgggcaggaa caaccagggc cggatgacca tgcgttaccg gggaggcggc cacaagcggc
    21841 aataccgggt catcgatttt aaacgggata aggacggagt ccccgctacc gtgcactcca
    21901 ttgaatacga tcccaaccgt acggcttata tcgccctatt gcattacgcc gacggggaga
    21961 aaagatatat gatcgcgccg aatggcctga aagtgggcca gaaagtgatg tccggcaaag
    22021 atgccgaacc ggaagtgggc aatacccttt acctccggga tgtgccgctc ggcacctcca
    22081 tccacgcgat cgaattgcat ccgcaccagg gagccgccct ggcccgcagc gcggggacct
    22141 atgccgaact gaccggcaag gaagaccgtt atgccgtggt caagatgccg tccggggaag
    22201 tccgccggat cctgatccag tgccgtgcca cgattggcac gacttccaat cccgaccaca
    22261 gccagactat tttgggcaaa gccggaagaa gccgccacag ggggatccgc cccaggaacc
    22321 gcggggtggc catgaatccg gtcgatcacc cgatgggggg tggggaagga cgtgcctccg
    22381 ggggccatcc caggtcgaga aatgccaaaa aagctaaagg ggccaagacc aggaataaga
    22441 ataaaaaatc agaaaaactg gttatctccc accggaatgc taaaaaatct caaggtaaat
    22501 aaagaagcaa gtcatggcaa gatcaattaa aaaaggacct tatgtttttc acaagctgct
    22561 gaaaaaagtg agcgtgaaca aacaagccaa taaaaaaacg gtcatcaaaa cctggtccag
    22621 ggcatcaatg atcatcccgg acatggtcgg ggagaccatt gcggtgcaca acggcaaaac
    22681 ttttatacca gtatatgtca cggaaaacat ggtcggccat aaactgggcg aattttctcc
    22741 aacccgcacc ttccggggcc attcgggcaa ccgtaaggac gaaggcaaat aaatcaacta
    22801 atcaccaaaa tttgtagatc atggaagcag tagctcattt gagaaatggt catggatccg
    22861 cccgcaaaat caggctggtg gtggacaaca tccggggtaa aaaagtggtg gatgccctca
    22921 acatcctcaa gtttaccaaa aaagattcag cctactgggt ggaaaaggcg ctgatttccg
    22981 cggtggccaa ctgggaaatc aaaagcgaac aatccgggaa tacggatgct tatgacctgg
    23041 tgatctccaa agcctttgtg ggcgggggac cggttttgaa aagatttcag cctgccccgc
    23101 atggaagggc ccaccgcatc cggaaaagat caaatcacct caccctggtg gtgagcaatt
    23161 cggtgccgct gccttcggag gcacagcagg aaccggaagc acaagaagca caggaagcat
    23221 aacaattaac cagaatattt tataaaaatc atatgggtca aaaagtaaat ccgataggaa
    23281 gccgactggg aatcatccgg ggatgggatt caaactggtt tggtggcaag tccatggcta
    23341 agaaagtcgt tgaagacgaa caaatcagga aatacctctt cgcaagggtg gaaaaaggcg
    23401 ggattgccaa agtagtgatc gaacggacac tcaaaagaat caccctgacc attcatactt
    23461 ccaggccggg catcatcatc ggaaaaggcg gtacggaagt cgacaagatc cgcgaggaac
    23521 tgaaaaaact ggccggcagc gatgtccaga tcaatatcat ggagatccgt aaacccgaac
    23581 tggaagcgac catcgtcggc gaatccatag ccaaacagat cgaatccagg atcaattacc
    23641 ggagagcgat caaatcgtcc atccagtcca ccatgcgggc cggggccgag ggcatcaaag
    23701 tccggatttc cggccggctg aatggagccg agatgtcccg ttcggaagaa tacaaggaag
    23761 ggaggacccc cctgcatact ttccgggccg acattgatta tgccctggcg gaagcacaga
    23821 cggtgtatgg aaagatcggc atcaagacct ggatttgtaa aggagagatc ctgaccaaac
    23881 gggatttgtc ccccaatatc ggcctggtta agaaagattc agcgggcggc gatcgcaggg
    23941 atgaccgtgg tggaagaaga cgcccgggtg gcggaggtag aggagaccgt ggccgcggag
    24001 aaagaggagg ggatcgggac gatcgcggag gccgtggaag gagaaataac caggattaat
    24061 tttgcaaaaa cgatgtacct ttgccaaact tttttcaaaa ataagacatg ttacagccta
    24121 aacgacagaa ataccgtaag caacagaagg gcagaaaccg gggggttgcc gtcaagggaa
    24181 gcaccatttc ttttggttct tatgccttga aatccatgga gttaggaagg atcaccaacc
    24241 ggcagctgga agcagcccgt atcgcgatga cccgcgaaat caaacgtgaa ggccaggtct
    24301 ggatccggat tttcccggac aagccgatta ccaaaaaacc cgcggaagtg aggatgggta
    24361 agggaaaagg tgccgtagaa tattttgtcg ccctggtcca gcccggcagg atcctgtttg
    24421 aaatggacgg cacttccacg gaagccgcca ccaaagcgct gaatatggca gctcagaaac
    24481 tgcccatcaa aacaaaaatt gtcatccgtc acgatttata ataacgatca ccatggccaa
    24541 taaaaagaat caggatataa aggagatgtc tcccaccgat atcagcaaca gtctgatcga
    24601 aacccggata gacctgcaaa aagcccggtt tacccacgcg gtcaaagggc tcgacaatcc
    24661 caagcagatc accggcatga ggaaaaaaat tgcccgtctg aaaacggaag aaagggcccg
    24721 ccagatccag gcgatgaccg aggcggaaaa agccggaaga tccaaaatca gggccagaag
    24781 aagcaaatca taatgaagga cctcgtcctt tgaaaaatat aagacatgga acagaattca
    24841 gaagtaaaag agaaagcgcc caggaaaatc cgcaaaacca gggttggagt ggtcaccagc
    24901 gccaaaatgc agaaaagcat taccgtttcc ctggatataa aggtcatgca cccgaagtat
    24961 ggcaagtttt tgcacaagac caccaaattc atggcccacg atgaaaaaaa tgaggcccgg
    25021 gaaggggata ccgttaaaat catggaaacc cggcctttga gcaaaaacaa aagctggagg
    25081 ctggttgaaa tcattgagcg agccaaataa tccaaccatt gcaagaattt attttaaatc
    25141 aacagggatc atgatacaga ctgaatcaaa attaaatgtg gcagacaaca gcggggccaa
    25201 gatcgtgcag tgcatccggg tgttggggag cacgcgccgc aggtatgccg gggtgggcga
    25261 tgtgatcgtg gtttcggtga aagacgccac gccgaccggc gggataaaaa aaggaacggt
    25321 gtccaaagcg gtcatcgtcc ggaccaccca gcccattcac cgcaaggacg ggtcctacat
    25381 ccgttttgat gacaatgccg tagtgctcct gaccaacgcc gatgaaccca gaggtacccg
    25441 tattttcggg ccggtcgccc gggaactccg cgacaaaaat tatatgagaa tcatttccct
    25501 tgcacccgaa gtattataat taacaggact gaatccagca gatatgatca caaaatcaaa
    25561 aaataaaatc cggcgttttg cccccaaact taaccttaag aagggagacc aggtcatcgt
    25621 catttccggt gatcagaaag gcaaaaaggg agaagtgcag aaggtgatga aagaaaccct
    25681 taaggccatt gtgaccgatg tcaatatggt caaaaggcat accaaaccca ccaacaacaa
    25741 tcccgggggc atccgtgaaa tacccgcccc gatctatatc tccaagctta tgctgctgga
    25801 tcccaaaagc ggggagccta cccgcgtcgg ccgccggacc gaaaacggca aatccgtgcg
    25861 gtatgcaaaa aaatcaggtc aaacgattaa gtgattgagt tatgaaatat acacctagac
    25921 tgaagcagca atataccgaa gaggtggtgc ccaaattgaa ggagcaattc aattataaaa
    25981 ccatcatgca ggtcccccgg ctgatgaaaa tctgcatcaa ccagggagtg ggcgaagcca
    26041 cccaggaccg caaagtcgtg gatatcgcct gtaccgagat atcccagatt acgggtcaga
    26101 aagccgtgcc caccaaatcc aagaaggcca tctccaattt caaattgcgt gaaaacatgc
    26161 acattggggc ccgggtgacg ctccgcggcg ataaaatgta tgaattcctg gaccgcctga
    26221 tttcggttgc cttgccccgg gtaagggact tcaggggtat caatgataaa agctttgacg
    26281 gccggggcaa cttcagcatg ggcattaccg aacagatcat cttccctgaa attgacctgg
    26341 acaaagtgaa taaaatttcc ggaatggata tttccattgt gacttcggcc agaacggata
    26401 aggaggcctt tgccctcctg tccagcctcg ggattccctt taaaaaccag aaaaattaat
    26461 taccaccttc accatggcaa aaaaatcagt aatagcaaga caaaagaagc gcgaggaatt
    26521 ggtcgccaaa tatgccgaga cgagggccaa attgaaggca gccggtgact atgaagccct
    26581 ggataaattg ccgaggaacg cttccccggt tcgtttaaaa aaccgctgtc agctgacggg
    26641 caggcccagg ggatatatcc ggaaattcgg actcagccgc gtggccttcc gggacatggc
    26701 gctttatggt aaaattccgg gcatgaccaa atcaagctgg taattcatca atatcattca
    26761 agaaatcgtt tatggcagtt acagattcaa tagcagattt tctgacccgc atccggaatg
    26821 cccagcagtc cggtcatcgt atggtggaaa tcccggcttc caatgtcaag aaaagaatga
    26881 cggaaatcct ctatgaaaac ggatacatcc tcaaatacaa gtttgaaaaa ccggaaggcg
    26941 aatttgaaaa aatcatgatc gccctcaagt acgatccggc aacccgtcag ccggtgatcc
    27001 agaaactcca gcgggcctcc aggccgggat tgcgaaaata cagcggggcg gaggatctgc
    27061 cccgggtctc ctcgggcctg ggcattgcca tcgtatccac caacaaagga atcatgacgg
    27121 acaaacaggc ccgcgcccag catgtgggcg gcgaggtatt gtgttatata ttctaataaa
    27181 ttaaaggttc tgaatatgtc gcgtattgga aaattgccga tcagcctgcc aaagggcgtt
    27241 gaggtgaaaa tagataaaaa ccgggtcacc gtaaagggac ccaaaggcag cctggtccag
    27301 gaagtggact atgacatgaa ggtgaccgtt gaagacggga ccttggtcgt aagcaggcct
    27361 tctgatcaga aaagacataa agccctccac gggaccacca gggcgattct tgccaatatc
    27421 atcaaagggg tcagtgaagg atttaatcag gatatggaga tcgttggcgt gggttaccgg
    27481 acctcgaata ccggcaatct cctggagatc ggggttggac attcgcaccc catcatgttc
    27541 tatgtaccgg atgaactcaa agtggaaacc ctgaccgaga aagggaaggc ccccctgatc
    27601 aaattgtccg gcacggatcg tcagttgctt ggacagatca gcgccaaact caggactttc
    27661 cgcaagccgg aaccttacaa ggggaaaggc atcaagtacg ccggggagat cctccgccgg
    27721 aaggccggaa aggcagccgg aaaggctaaa taataaaaat tgaaggtgaa acagcataaa
    27781 ctgatttgag atgaaatcca ctaaaaatta cgggaagcaa aagattcggt accggatccg
    27841 taaaaaagtc tccgggagcg ccgtccggcc ccgtttatcc gtgttcagga gcaacaaatc
    27901 catttattgc cagttgatcg atgatgcgac cggccatacc ctggcttcca cttcctcgca
    27961 cgacgaaacg gtcccgaaag aggtcaacaa aacggaacag gcaaaggcag tgggcaatct
    28021 tttggcttca aaagcaaaag ggctcggtat agaaaatgtg gtttttgacc ggggtggcaa
    28081 tttatatcac ggaaggatca aagccctggc cgatggcgcc cgggaaggag gtcttaaatt
    28141 ttaattcagc ataagaatat ctttaaaagc atatggcaaa tagagttgtg aaaagggtaa
    28201 aacccagcga agcggaactt aaggaaaaaa tggtcgcgct caaccgggtg gccaaggtca
    28261 ccaaaggagg ccgtaccttc agttttgcag ccatcgtggt ggttggagat ggcaatgggg
    28321 tggtgggtca cggtctgggt aaagcccggg acgtgcagga atccatcgcc aaagccgtgg
    28381 atgatgccaa gaaaaacctg atccgtgtaa atatccacaa agggaccatc ccccatatac
    28441 aaaccggcaa atacggcgca ggccgcgtac tcatcaaacc ggcctctgaa ggtacggggg
    28501 tcatcgccgg cggcgnnnnn nnnnnaccgg tcaccggtac aacgacgtca taggctacgt
    28561 catcgccggc ggcgccatga gggccgtcct ggatatggcc ggagtgcata atgtattggg
    28621 taaatccctg ggatcatcaa acccgcacaa cgtgatcaaa gccaccctgg atgcactctc
    28681 taaaatgaga tcgccccagg aaatcgccca gcaacgcaag gtcgccctga ataaagtgtt
    28741 taacggttaa tatcagacag tatgacaaag attcgaatca cccagacgaa aagtatcatt
    28801 gaaaagaccg atcgtcagaa aaaaacgatc aaagccctcg gcctgaaagg ggtccggcac
    28861 agcgtggagc agcaactcac gccccagatc gaaggcatgc tttccaaagt cgcccacctg
    28921 gtatccgtgg acaagatttc ttaaattctt ttaaagcaca gaagatcatg gaattgaata
    28981 atttaaaacc ggctaaagga gctaccagga acaataaaag aaggggccgg ggcattgcca
    29041 ccggttcagg gggaaccgcc ggcaggggac ataaaggaca taaggcaaga tcaggtcata
    29101 aggcaaaaag acattttgaa ggcggccaga tgcctcttca aatgcgcttg cccaaaatcg
    29161 ggtttaggaa tccagcgcgg gaagaatttg ccgtgctcaa cctggaccaa cttcagaact
    29221 atgccaccaa atccggtcaa aaccagatcg acctgccctg gctgattaag gccggcgtac
    29281 tcaaacccgg tcaaaaaatc aagatactgg gccgcggcga actggcctcc gggattcagg
    29341 tgactgccca tgcctgttcc gcatccgcca aaaaaatcat tgaagagaaa ggcggcacga
    29401 tcactctcat ttaattcccc cccgatgaat agcttgatac agacactgaa aaacatctgg
    29461 aaaatagaag aattgcgcaa gcgaattcta tttaccctgg ccgtattggc ggtcttcaga
    29521 tttggttctt atatcgtgct tcccggggtc gttgccggcc agttgcaggc ggccgcaggc
    29581 aaccgcggcg ccaatgacct gctcaacctg gtgaatattt atacgggagg cgcttttaat
    29641 aacgcatcca ttttcgccct gggcatcatg ccttatatca cggcttccat cattgttcag
    29701 ttgctgggtt ttgcccttcc ctatttccag aggctgcagc agcatgaagg ggaatccgga
    29761 cgtaaaaaac tcaaccagat cacccgtatc cttaccgtgg tgatcaccct gcttcagggc
    29821 ggaacttatc tggcttatct caacgcatcc aacgccattg atccttcggt tcccaaaagc
    29881 gtattctatt ttgtaaattc catcctgctg gctaccggaa cagtgacggc aatgtggctg
    29941 ggtgaacgga tcaccgacaa agggattggc aacggggtct ccctgctcat catgatcggc
    30001 attattgccc gtctgccaca ggccgtgatc tttgagattc aggcacgttt cagttcaggc
    30061 ggtaccttta tgctgatcct tgagctggcc gctttgatcg tggtggtcct gggctccatc
    30121 ctgctggttc agggggtaag gaaaattcca atccagtttg ccaagcgcat ggtgggccgt
    30181 acggggctgc ccgtgagcgg caacagggat tacctgccca tcaaactcaa tgctgccggg
    30241 gtgatgccca tcatttttgc ccaggcgatc atgtttttgc cgggtttcgc cctgcaatcc
    30301 ctttccgggg ccgcggcctc cagcagcggt tttgtccgtt ctcttaacga ttttacctcc
    30361 ataccttata acgtcattta ttttatcctg gtcgttttgt ttacttacgt gtatgtggcg
    30421 ctcatcgtca acagcaccca gtattcggaa taccttaaaa ggcagaatgc ttttattccc
    30481 ggggtaaaac ccggttctcc taccgcggaa ttcatcgata ccattacctc gaaaatcacc
    30541 cttccgggcg cggtcatgct gggtctcctg gccatcacgc cggcaatggc caccgctttt
    30601 ggcatcaaca acgccttcgc ggcctttttc gggggatcca ccattctgat cctcgtaggc
    30661 gtcgccctgg ataccttgca gcaaatagaa agttacctgc tgatgaggaa atacgacggg
    30721 ctcgtaaaat ctggaaacat caaggggcgc accggcagcg gaatggcacc aataggggca
    30781 ggctcctaac cggccccact caaaatcagg atgacaaaag gcaaagttgt aattaagaca
    30841 gaagaggaga ttgaactcat ccggcacagc agccggatcg cctgcgaagc cattgccgaa
    30901 gtcgccaaag tcatccggcc cggttatacc gggatcgagg ccgacaaact ggcagatacc
    30961 tatatcaagg accatggggg tttgccctca ttcaagggat atgggcatcc cgccttcccg
    31021 gcttccctct gtatctccat caatgaagcg gtggtccacg gtattccgac ccacacgcct
    31081 ttccgggaag gggatctcgt atctttagat tgcggcgcat atcttaatgg ttttcatgga
    31141 gatgtagcgt acaccctggt cctgggggag gtccccgaag cggtgatgaa gttatgcgtg
    31201 gtcaccaaac gcagcctgta cctgggtatt gaacaaagcc gtcagggcaa ccggctggga
    31261 gacataggtt ttgccatcca gcaatatgtg gagaaggaga acggtttcgg ggtggtccgg
    31321 gaactggtcg gccacggagt gggtaaacag ctccacgaac ccccggatgt gagcaattac
    31381 ggaaaaagag gcaacggcct tgtccttcag gagaatatgg tatttgccat cgagcccatg
    31441 gtcaatctgg gcacccgtca gatccggacc ttgtctgact attggacggt ggtttcaaag
    31501 gacaacaagc cctctgcaca ttacgaacac accgtagccg tgagaaaaac cggcggagaa
    31561 acgctcaccg accatacggt gatcgaagag gccattaaaa ataataaaga attaagcgaa
    31621 atttcgctaa aaatatagat atttgcagtc cgaaatttta aaggatgcct aaggaagact
    31681 tgatcaagca agatgggatc attgaagaag cattgtccaa tgcgatgttc agggtccatc
    31741 ttgaaaacga tcacgtcatt ttagccacca tttccggtaa aatgcgcatg cattatatcc
    31801 ggttattgcc gggtgacaaa gtggccgttg agatgtctcc ttatgatctt tcaagaggca
    31861 ggattattta ccggtacaag taaaacactg aggatcatga aagtaagaac ttccatcaaa
    31921 aaaaggtcgg ccgattgcaa aatcgtacgc cgcaagggca agctttatgt catcaacaaa
    31981 aagaacccca aattcaaaca aagacaaggt taaaaattta attcatggca cgtatttcag
    32041 gcattgacct tccaaaaaac aaaaaagggc tcatcggcct tacctatatt ttcggcatcg
    32101 gcacctcctc ttcggccgcg atcctggaca aagccgggat cgatccggac atgaaagttt
    32161 cggcctggac cgacgaacat gtgcataaaa tcactaaaat catccaggat gattataaag
    32221 tggaggggat gctccgttct gaagtccagc tcaacatcaa aaggttgatg gacatcggtt
    32281 gttaccgcgg gatccgccac cgcaggggac tcccggtcag agggcagaat acccagacca
    32341 atgcccggac ccggaaagga aaacggaaga ccgtggccaa taaaaagaaa gtaaccaaat
    32401 aaccgtcaat cagatccaga tatgtctcag gtaaaaaaag ccaagaaaag gaaagtgcat
    32461 gtggagtccg aagggattgc ttatgtcctc gccagtttca ataatgtgat cgtcaccatt
    32521 accaacaaac agggacaggt ggtctcctgg tcctcgtccg gccgcgaagg cttccgggga
    32581 tcaaagaaaa acacccccta tgccgcccaa aaaaccgcgt cccatgccgc caccctcgcc
    32641 catgaggcgg gtgtccgttc cgcggatgtt tatgtaaagg gaccgggtac cggccgtgaa
    32701 gccgccatcc ggggcatcaa cgacgtcggg atcaaagtga ccaccatcac cgatacgacc
    32761 ccgatccccc ataatggctg ccgcccaccc aaaaaacgca gggtataatg caaaccgaaa
    32821 ccaggatcat ttaatcaaca ttttcagaat atcatttaat catggcaaga tataccggac
    32881 caagtacaaa aatcgcaagg accttcggag agcccatttt cggccaggat aagagctttg
    32941 acaggaaaaa atacgcgccg ggccagcatg gccagagccg caaaaaaaag cagaaatccg
    33001 actacgccgt ccagcttaag gaaaaacaaa aggcaaaaca tacatacggc atcctagaaa
    33061 ggcagttccg gaaaacgttt gaactggcca gccgcaaaca cggcgttacc ggcaccctgc
    33121 tgcttcaatt gctggagtcc aggctggaca ataccgtgta tcgcctcggc atcgccccta
    33181 cccggagggc cgcccgccag ttggtatccc acaaacacat agccgtcgat ggtaaaatcg
    33241 tgaatattcc ttccgccctc cttaaaccgg gtgatgtggt cgccgtaaga ggcaaatcgg
    33301 ccaacctgga acatatccag aaccaggtcc atgccaaaac cgatatccgc aaatcgccct
    33361 ggctggaatg gaatcctgaa cgcatggaag gcaaattcct catctacccc gaaagagaca
    33421 tgataccgga acggatcaac gaacagttga tcgtcgagtt atattccaaa taacccgaag
    33481 gaccgatttc ttaaccagtt ttttaagcac aattgatttt atgagcatat taaatttcca
    33541 aaaaccggaa aaaatcattc tgcaaaaagc caatgatttt gacggtgtgt tcgagtttaa
    33601 accgcttgaa ccaggatttg gtcagactat aggcaactcc ctgagaagaa tcctcctgtc
    33661 ctccctcgaa ggacatgcca tttccggcat ccgtatcgcc ggagtagacc acgaattttc
    33721 caccatcaaa ggagtggttg aggacatcgt ggacatcatt cttaacctga aacagatccg
    33781 cctgaaaaag gtcaacaata ccgacgacaa ttccgaagac accatctacc tgaccattaa
    33841 ccgcaaggac gaattccggg ccggggaaat tgaaaaattc accaacgtgt acaaggtgat
    33901 gaaccctgac ctgctgatct gcaggatgga gcccttcgtc aacctcgaaa ttgaactaaa
    33961 gattaccaaa ggcaggggtt atgtccccgc cgacgatacc tatcccaagg acgccccgat
    34021 cgggctgatc ccgctggatg cgatctatac ccccatccgc aatgtggcct attccatttc
    34081 caatacccgg gtgggccaga ggacggatta tgaaaagctg accattgaat taaaaaccga
    34141 cgggaccatc cacccggaag aagccatcaa ggaggcctcc aggatcatga tccagcacct
    34201 gatgctgatc accgatgaaa acatcacctt caacgatgcc tcccggaagg aagacgccat
    34261 cgtggacgag catatcctcc atatgcgcaa attgctgaaa acctccctgg aggacctgga
    34321 tctttcggtg cgcgcctaca attgtctgaa agcggccaaa atcaataccc tcggggaaat
    34381 ggtcaaatac gatacccacg aactgcttaa attcaggaat ttcggtaaaa aatccctggt
    34441 ggaaatcgaa gaattgctgt ccacgaaggg cctgagtttc ggcatggacc tttccaaata
    34501 caaactggac gaagaataat attcattcaa tcaacccatg ccaattagcg tgtgagcatt
    34561 ccgttgctcc gctgtggcga ggataaaaac atttcatcat gagacatggc aataaaatca
    34621 atcacctcgg ccgtaaaatc ggccaccggg cggcattgct caagaacatg gcggcatccc
    34681 tgatcctgca caaaaggatc aacaccaccc tggccaaagc aagggaactt cgccagtacc
    34741 tcgagcccct ggtcaccaag tcgaaggaca ataccaccca ttccaggagg gtgatcttca
    34801 gctatcttca aagcaaggat gcccttaaag aattgtttgc ggtgattgca ccgaaaattg
    34861 ccacccgtcc cgggggctac ctgcgcgtca tcaaaaccgg atttcgcagc ggggacgctg
    34921 ccgaaatggc cctgatcgaa tttgtggatt ttaacgaagt gtataccctg gggaccaaac
    34981 cggcatcggc tgccgcacct gaaaagaaaa agcgaacccg ccggagcagt acaaaaaagg
    35041 ccaaagcgca aggcgaacaa accgagacgg aagaagccaa gaaggaagaa tgaggccaat
    35101 ttcatttttc cgttcaagga tatgaataaa caaaggggat tgagctttca atcccctttg
    35161 ttttacaaat cgtttagatg aaacaatcac cggccatttt tcttaaattt cttaccgggg
    35221 cacgggccgg caccccggtt taagttttat atttgtggac gttttttcaa ttccagccag
    35281 atccatgtca gaagcatacc aatcccctgc tccggcagtc cttttgttgc aagacggcaa
    35341 agtttttcac ggagaagcga ttggagtgaa gggtacagct tccggagaac tgtgttttaa
    35401 cacgggcatg accggttacc aggaaatctt tacggatccc tcctattatg ggcagctgct
    35461 gatcgccacc aatgtccata tcgggaatta cgggaccaaa gccacggaaa cggaatcttc
    35521 atcggtcaaa attgccggat ttatctgcaa gaactttacc actgattttt cccgccttca
    35581 ggccgagtca tcgatccagg attattttgt ccgcgaaaaa ctggttggga tccaacaggt
    35641 ggatacccgg gaactcgtgc gccatatccg caacaaaggg gccatgaacg ccgtcatttc
    35701 ttccgagatc ttcgatcttg ggcggctgca ggaaatcctc gggaagacgc ccaatatgga
    35761 tggcctggaa ttggccagcc gggtcagtac caaagaggaa tattttctcg gagactcgtc
    35821 ggcttcttat aaaatagccg tcctggactt cggcaccaaa cggaacatcc tgctcaacct
    35881 ggtcaacagg gattgttacc tgcgtatttt ccccgcggaa accccggctg aaaaaatact
    35941 ggcctgggga gccgacggtt attttttatc caatggtccg ggggacccgg ccgccatgga
    36001 ttatgccata gaaagcaccc gggccatcct gaagaccggc aaaccgctgt tcggaatctg
    36061 cctggggcac cagatcctcg cgcttgccct taatgtgccc acctttaaaa tgcatcacgg
    36121 gcaccggggc atcaatcacc cggtgaaaaa cctggtcagc ggcaaatgcg aaatcacctc
    36181 ccaaaaccac ggattctgcg tggatcccga aaccctgctc cagaaaacag acctgatcga
    36241 gatcacccat gtcaatctca atgacgatac ggtggaaggc atccggctca aaggccgccc
    36301 ggctttttcc gttcaatacc acccggaagc atccccgggt ccccacgacg cccattatct
    36361 atttgaccag tttattgacg aactggcaga ctataaaatg aataaatcct aatatgagtg
    36421 taattgttga tatccgttca agggaaatcc tggattccag gggaaatcca acggttgaag
    36481 ttgaactcga aaccgaaaaa gggaccctgg gtcgcgctgc agtcccctcc ggcgcttcca
    36541 cgggcaaaca tgaagccgtg gaattaaggg atgggaacaa ggaccgttac ctgggcaaag
    36601 gcgtgttgaa agcctgccaa aacatcgaag aaaccattcg tgaagagctg atcggggtgg
    36661 aggtgcatga ccagaagtac atcgaccagc tcctgatcga actggacggt acttttaata
    36721 aatccaaact gggcgccaat gccaccctgg gcgtttccct ggccgccgcc aaagccgccg
    36781 ccctggaaac caaccagtcc ctttaccggt accttggcgg ggtcaatgcg catgtcatgc
    36841 cggtacccct gatgaacatc ctcaatggag gtgcccatgc agacaacaaa atcgatttcc
    36901 aggaatttat gatcatcccg atcggggccg actttttctc cgaggctctg aggatgggag
    36961 tggaggtttt ccataacctc aaatccgtac tcaagtcgaa aggatacagc accaatgtgg
    37021 gggatgaagg aggcttcgcc ccgaacctca attccaatga agaggccatc gaaatcgtcc
    37081 tggaagccat ccaaaaggcg ggctacaaac cgggtgaaga cattgccata gccctcgatg
    37141 ccgcggtatc cgaactgtgg gatgaggaaa aacaacgata cgtatttaag aagtccaata
    37201 aaatggaatt cgataccccc ggcatgatct ctttttggaa agaatggtgc cataaatatc
    37261 ccatcctgtc catcgaagac gggctccacg aggatgactg gagcggctgg agcgccatga
    37321 ccaaagaatt aggcaataaa atccagattg taggggatga tttttttgtc accaatacgg
    37381 atcgcctgca aaaagggatc gattccgatg cggccaattc catcctgatc aaggtaaacc
    37441 agatcggcac cctgtcggaa accatcgacg cggtccagct ggccaaccgg aatgcctata
    37501 ccagcatcat cagccaccgt tccggggaga cggaagatac cttcatcgcc gacctggccg
    37561 tggcgctcaa taccggtcag atcaaaaccg gttccgcttc ccgttccgac cgggtggcca
    37621 aatacaacca gttgctgagg atcgaagagg aactgggcga atccgcagta tacccaggca
    37681 aggcctttat gataatataa gatatatttt taatatattt atgcttcaat ttcacttgaa
    37741 tgcttccgat taaggctatt actgcttcgc tccggttcca tttgtccaaa atactgccgg
    37801 actacctggt caaccgctat tttatcaccg ccatggtctt ttttatctgg atgatttttt
    37861 tcgataaaaa caaatgggtg aatcaatggc agctttcgca gtcaatatac cggctggaac
    37921 aggaacagaa aatcctcgcc ggaatgattg tagaagcgcg gagggacaag gaggacctgg
    37981 agaaaaacaa ggagaaatac gccagggaaa aatattacat gcataaggaa aatgaagaca
    38041 tttttatttt cgaagaaaac ttaaatcctg ataaatgagt gtattggtca accgggacag
    38101 cagggtcatc gtgcagggat ttacggggaa agaaggcact tttcatgcca cccagatgat
    38161 cgaatacggc acggccgtgg tgggtggggt gacccctggc aagggtggtc agcgccacct
    38221 cgaccggccc gttttcaata atgtggaaga cgcggtcaaa gcagcgggag ccaatgtttc
    38281 catcattttt gtcccaccgg cttttgccgc ggatgcgatc atggaaagcg cggcggcagg
    38341 catcggggtc atcgtttgca tcacggaagg catcccggtc caggacatgg tccgggtaaa
    38401 agaatatatc agggactttc cctgccgtct catcgggccc aattgcccgg ggatcatcac
    38461 cccgaatgag gcgaaagtgg gcatcatgcc gggattcatt catcagaaag gccatatcgg
    38521 cattgtgtcc cgttcaggca cgcttaccta cgaagcggtt gaccaggtga ccaaagcggg
    38581 catgggccaa tccacctgca tcggcatcgg cggggacccg atcgtaggca ccaccaccct
    38641 ggatgcggta caactcctca tgcaggatcc tgaaacccat ggaatcatca tgatcgggga
    38701 aatcggcggc ggtatggaag ccgaagcagc ctattggata aaggaacacg gtaccaaacc
    38761 cgtcgtggga ttcattgccg gccaaaccgc ccccaaaggc cgtaaaatgg gacacgcggg
    38821 cgccatcatc gggggtgcgg cggataccgc cgaagccaaa atgaagatca tgtcggagtg
    38881 cggcatccat gtcgtgaaat ccccggccga aatcggcgaa agcatgcgga ttctgatgaa
    38941 gcagtaatca gaagtcaata gtcaacagtc aacagtcaat agtcaatagt caatagtcaa
    39001 tagggaaatt cgggagtaag gtcatttttt agaccgaccg aattttattt tcttgt
//
LOCUS       DAOQXT010000039        37146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1294_length_37146_cov_6.695910, whole genome shotgun sequence.
ACCESSION   DAOQXT010000039 DAOQXT010000000
VERSION     DAOQXT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 37146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37146
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1294_length_37146_cov_6.695910"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1080)
                     /locus_tag="PKM27_08745"
     CDS             complement(133..1080)
                     /locus_tag="PKM27_08745"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNR07389.1"
                     /translation="MNKQAFRFFLLVQLFVFTWFAGAQTTLTLAPSADNTLYESSSGN
                     ISNGKGTKIFAGKTKNGLIRRGLVKFDIAGQVPPGATLSGVRLSLTMDRTITGPQPVS
                     LHGLTREWGEGISMADGEEGGGAAAQNGDATWVHALWPGSPWSKPGGDFIGNASASIQ
                     VDGDGIYSWSSAQMIKDAQRWLDTPSVNFGWCIVGNEVSSTTAKQFASRESLSATNRP
                     MLTITYHTLSTSTGKDPGLSAVQVFPNPSTGKFFLINHGRPPVSIAIFNSLGHRVYAR
                     TLTVEQNELDLRSQPQGLYWYRIEYGFNKAGTGKLIIYD"
     gene            complement(1108..1566)
                     /locus_tag="PKM27_08750"
     CDS             complement(1108..1566)
                     /locus_tag="PKM27_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07390.1"
                     /translation="MRTMISPAVLFMLMAGFLTGCAKNTSYEIEKIHAVLYRLIDADN
                     RSDLETILNAYSDSIEFYPAGKAFIQGISTVKKNYETLLKAYKMNLQTQILETQLFSD
                     HALVTGINKGFRTSLDDSVQYRVDDKYIALMVRNKKGDWKIDKLIWGLYH"
     gene            complement(1701..2168)
                     /locus_tag="PKM27_08755"
     CDS             complement(1701..2168)
                     /locus_tag="PKM27_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR07391.1"
                     /translation="MENHDMDAKITIRKYTPSHRAVLLQLFRLNTPRYFSITEESHFI
                     DYLENHAGHFFIVESGGAVVGCGGYNLFPEQADARISWDLFHPDFQGRGLGSALLQYR
                     IDRINEVPGLKTITVRTSQWVYRFYEKFGFELKEVIKDYWAEGFDLYRMVRPL"
     gene            complement(2205..2903)
                     /locus_tag="PKM27_08760"
     CDS             complement(2205..2903)
                     /locus_tag="PKM27_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNR07392.1"
                     /translation="MKWYKGKVAFFHPAIQPGLKILLVCLSGWLACKQAVGPEAGSGS
                     LNLPDSGISAQAAGSGPAFPDHWPESFGFGHAVSETEIEAWNIDIMPDGTGLPEGQGK
                     VEEGRLVYAQKCILCHGATGTEGPFDRLVGREPREGFPFGREYRYLSMRTIGNYWPYA
                     TTLFDYVRRAMPQNEPGSLSDHEMYAITAYLLHLNEIIPQDAEMNRLTLPAVKMPARD
                     RFVYDNRQGGNEIR"
     gene            complement(2890..4161)
                     /gene="soxC"
                     /locus_tag="PKM27_08765"
     CDS             complement(2890..4161)
                     /gene="soxC"
                     /locus_tag="PKM27_08765"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HNR07393.1"
                     /translation="MMKQKSTKGSFFSRRYLLKLSAAALGGAFVRTAAEAQVSTTPAN
                     SKANDPTRIPGRGSSKLGSRSPDEHLERLVLRPSPSGESMTPIQNLSGIITPADLHFE
                     RHHGGIPSIDPESYELLIHGLIKTPKKFTLKDLKRFPQHSRICFIECSGNGWSTYFKK
                     DITEAVTPQRMDGLISTSEWTGVMLSTLFREVGIKPGAKWFLAESSDAAVMTRSIPME
                     KGWEDAMIVYGQNGEAIRPEQGYPARLLLPGYEGNTQVKWLRRIEVSDQPFMTREETS
                     KYSDPLPDCTARLFSFVMDAKSLITFPSYPYILPDKGWWEIQGYAWSGRGKIIRVEVS
                     TDGGKHWFPAELQEPVLSKCQTRFRYLWNWKGGTAILMSRAMDETGYQQPTLGQLEKV
                     RGKGTFYHFNHIRAWKVEKDGSLKLGLNEMV"
     gene            complement(4288..5082)
                     /locus_tag="PKM27_08770"
     CDS             complement(4288..5082)
                     /locus_tag="PKM27_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR07394.1"
                     /translation="MKKMPAHTISSLTKAFGALFLLTLLPFTGSAQMGVGAKPAKGAE
                     VLFDGSREMLDQKWTYWAGPRLSATLPLKWAIVKDPVDPGTAVNSNDPSAAGGKYGSA
                     DIVTKKKFRDFRLHLEFLIMKPGGNSGVYLQNRYEIQILDGDSTKHGLGAVINETESP
                     YYAYHGPGKWNSYDLVFRAARFKDGKRTEKAMVTLYFNGKKVHTNQTINQVWGGPNSG
                     VDGGNDGGKGITDTPEGLKLQAEGHDVLYRNIWIKELKLKKPNTDF"
     gene            complement(5259..6188)
                     /locus_tag="PKM27_08775"
     CDS             complement(5259..6188)
                     /locus_tag="PKM27_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07395.1"
                     /translation="MISGHVFHPFYSIGLLILAALFNCQARLDKTARVYKSSEQVTGY
                     HFAKPDQVLELPASLHEISGIAVMDSTTLACIQDEKGIVYIFNSTSGQIINQYSFYGQ
                     GDFEDIAKFGNAMYILRSDATLFEIADFSGEPAKAISYGLNIPAYDNEGLCYDAGKGR
                     LLIASKGNSGKGEELKNRRDLYAFDLETKSLVEPAVVSFDERVIKKFALEKVSGIPMK
                     EKKSGEKEPDIKLRPTAIGIHPLTRQFFLLSAPDYMLFIFSADWTLEHLELLDPALFN
                     QAEGLAFYDNGDLLISNEGQDKKATLLRFNYKK"
     gene            complement(6341..7072)
                     /locus_tag="PKM27_08780"
     CDS             complement(6341..7072)
                     /locus_tag="PKM27_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNR07396.1"
                     /translation="MKICVAQTNPVKADIRQNINNHLAWTERALEHGTGMILFPELSL
                     TGYEPGLAQDLAMDPDDPCLDVFQQVSDLHQVTLGLGMPLKGTHGTYISQIFFQAHSP
                     RQVYRKQYLHQDEEPYFIPGKGQLFLSTGNLRIAPAICYEISIPEHAARVMQNGASVY
                     MASVAKTRQGVDHAVKRLPEIALQYSIPVMMSNCIGICDQFLAGGRSSVWNKEGLMVG
                     QLPEAREGLLIYDTATGEVSERLAG"
     gene            complement(7077..8450)
                     /gene="nhaA"
                     /locus_tag="PKM27_08785"
     CDS             complement(7077..8450)
                     /gene="nhaA"
                     /locus_tag="PKM27_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNR07397.1"
                     /translation="MMNDEKTHLTGKTPVERWILDPIQRFINNSTVSGLVLFSSALLA
                     LLLANSPWSDEFHHLWEHEFSIGYDGNIISKSLHHWINDGLMAVFFFVVGLELKREIV
                     AGELNELKKALVPVFAAIGGMALPALIYLYFNISSETASGWGIPMATDIAFALGVLYL
                     LGDRVPLSLKIFLTALAIVDDLGAVLVIAFFYTSDINFTSLATGGVFMAVLIISNLIG
                     VRNTLFYGIVGIGGLWLAFLMSGVHATIAAVMAAMTIPAKVKVSEAEYTYGLKRLMTR
                     FEAEKPNNKPTVTENQLLILDDIRQFSIAALTPLQRLEHSMHPLVSFIIIPLFALSNA
                     SVVLSAEALLQVVNPVFLGVFTGLLAGKVLGVFGITSVLIRLKWIQLPPGMNSRHLLG
                     AGFLAAIGFTMSLFISNLAFEDPALVEQAKMGILFASLLASVIGYLIIRFSRRGPGQN
                     SGQSPIS"
     gene            complement(8650..10071)
                     /locus_tag="PKM27_08790"
     CDS             complement(8650..10071)
                     /locus_tag="PKM27_08790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR07398.1"
                     /translation="MGWLDTGIGIFNYLILAFAILVMLSYLILAIISSFSLKAYLQKN
                     RYINYGALLALPHTPKVSLIAPAYNEGKTIRENVQSLLSINYNNFDVIVVNDGSKDDS
                     IPILVEAFELIPSDLPYHPQVPTKPVRAIYRSSNPSYSKLIVVDKENGGKADALNTGI
                     NISSHPYIVCIDVDCIMDKDVLLKLAKPFMEESGSRIIATGGVVRIANSCVIKSGRMV
                     KVNAPDNWLARIQVLEYLRAFILGRMAWARMDGLLIISGAFGMFDRDIVLRAGGYNTK
                     TVGEDMELVVRMRRYMIEHKLDYRVQYLPDPLCWTEAPESYKILRKQRSRWTRGTMET
                     LWIHRKMLFNPKYKILGLLSYPYWLIYEYLAPIIEFTGILISLILIIFGILSWKFFFL
                     FLFMVYSFALMFSAIAIFAEESTFKKYNTFRDLRKLAFIALLEPFIFHPFTVYAALRG
                     NWEKWRGSKGWGDMTRTGFNRPK"
     gene            complement(10077..10916)
                     /locus_tag="PKM27_08795"
     CDS             complement(10077..10916)
                     /locus_tag="PKM27_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07399.1"
                     /translation="MFPSANISYDGRISQAEWILLLGLAFALTALFFLAWTFIKRYQR
                     ILKARRKIQFTETIENTLFSILFNESPAPEALLKFRQGLAGNLLFKKMAIKTIVTLHH
                     NYSGTFRQKLEDLYFESGLSNYSLSKLKHRSWPEVVEGLRDLTNLNYYPAIKAIEQLT
                     YHPHEFVQKEAFLGLIRLRGLAELLKRKNRQLFLDDWMQSNILFALKSHQIPAIDHLD
                     TLLSSPNDSMVLLGARLIEHYQLTRYIPAIEKAMNISSNPKLKQELDDIASLLKTQER
                     TSI"
     gene            complement(10917..12152)
                     /locus_tag="PKM27_08800"
     CDS             complement(10917..12152)
                     /locus_tag="PKM27_08800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HNR07400.1"
                     /translation="MKKRILFCWAGLIGGLIFCAALSAQRQDPDSVMALAQKLAAGED
                     YKTAISLVTKLNEDHPENLDYKLYLARLYYWSKDYPRALEHLMPLTISEPVLAEAYDL
                     AIQVEYQLEHYPVVIEKCRKGHSLFPAGIPFYHFQEAMALEKTGEDREAVRLLDQIPK
                     SANNHRDAQYLKTQILRKEKNTLAAGYLNTSFYNPGFAPWHLAHLEYFHRNRSIALGG
                     RLNYGHLFGSDAVQVEADAYPRVGKGGYLYLNAGYSGADRIFPRYRLAGEYYQDINRY
                     NFSIGGRYLDFRVQKVQLLTGSAAINFGEWKFGYRNYLLHLESEWFVSHVLNLRRSLE
                     RRESYIQLDLQYGGTPYYFFASDAFSRISAWRAGINGMFRMGNHVFIQPVIMYEREEY
                     IPDTYRNRFNFQWIILKRF"
     gene            complement(12163..12528)
                     /locus_tag="PKM27_08805"
     CDS             complement(12163..12528)
                     /locus_tag="PKM27_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR07401.1"
                     /translation="MKVVLIEDDEMLRKSLSFFLKSKGHEVVEFENGLDPIDYIRKNY
                     ADISIIVTDLNLPFAGGRQVIHATRQIKDADIQIIVLTSSGVESTELEVFELGADDFI
                     SKPFSPAVLLKRIEKLTST"
     gene            complement(12525..15002)
                     /locus_tag="PKM27_08810"
     CDS             complement(12525..15002)
                     /locus_tag="PKM27_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR07402.1"
                     /translation="MDIGGKGEKQLEIERLKELLSFGILDTSPEKEFDDLAQLASIIC
                     DTPTALISFIDDSRQWYKAKVGMDLLQVPIEDTFCRHTIRQDTLLEIPDASQDDRVCT
                     NSFVTKENGVRYYAGIALKTEKGLPIGTVCVIDHKPRLLDEPQKKALEILASHTINLL
                     ETRKKNTRLGRELENVLKKKIEETQELLNLKEAEYNKLFQAITRSNGVVEFSPEGFIL
                     SVNKNFSEMTGYHEQELVGKHHTVLLDQEDQAKNLLFWQSLKSGAFQSGRFKRLRKDG
                     SVLYIQASYSPVLDSHEQVIRITKIAQDITMETHYRLALEKAKKIADDLNAQKDTFIA
                     NMSHEIRTPIHAIIGFTDLLLEKNLPEEFSSKLEAIKLAGDTLLYLVNDLLDLSKIEA
                     GLLQIEHLPYRLEEAVRDVFGMLRLKAAQKGLQFDYHFGPNLPEMVVGDKNRLIQILI
                     NLLGNAIKFTHHGAVHLDLRNEPPTGEPSNLHFTISDTGIGISKEKLDLIFERFTQAE
                     ANTSRKYGGTGLGLNISRLLIEKQGGWIRVESEPGKGSRFEFSLPCKIYFNESFAAAP
                     LRDSDRPNKSSARILMCEDNELNQRLVQSLFRNTPYQLDLAVNGIEGLNLLASKAYDL
                     VLMDIQMPEMDGYQAIRAIRTDLKSTVPVIALTAHSMVAEREKCLSLGMNDYLSKPFK
                     KEALLAKIEFWLNHSKELKEPPVLEGGKEEPLISLEQLEAFSGGDNDFQKEMLLLFRN
                     QSEKALRDMTTYLAGGDLINLGAMAHKLKSSFGMVGADLLLLENLETLASRKPAPDEI
                     MPVLSALENQLRQLHFEVEKILNNFKA"
     gene            complement(15196..15585)
                     /locus_tag="PKM27_08815"
     CDS             complement(15196..15585)
                     /locus_tag="PKM27_08815"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNR07403.1"
                     /translation="MKTEKPKSMKDYISSLPVKARKHMLQIRDLVKTLVPEAEETLSY
                     GMPAFNLNGHYLIYFGGYRDFVSLYPAPRNNPAFKEEISAFAGGKGTIRFPLNKALPI
                     RLIQKIIRFRLEENSNSVRKKRAAKKT"
     gene            complement(15749..16657)
                     /locus_tag="PKM27_08820"
     CDS             complement(15749..16657)
                     /locus_tag="PKM27_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNR07404.1"
                     /translation="MKAWLHLFLLLAISPVFSQQISDTSYRPPIPVPVYPPGQGPVIL
                     IDEGHNNFHTRDGRYQAFANLLERDGYKVQGYPGLFERGSLSRCKILVISNALNQTNV
                     ENWYLPTPSAFSQNEIDLVKNWVQEGGNLFLIADHMPMAGAAKDLAAAFGFEFSNGFV
                     LNTVTDGTAYFKNQDTTLAVNIVTMGRNEREKVSQIVSFTGQGFRLPREAVSILTFGE
                     NFENFLPDTAWVFNETTPRSNIKGWSQCAYRTFGQGRVVVAGEAAMFTAQLAGSLSVK
                     VGMNHPEAAQNHQLLLNIIHWLDGKL"
     gene            complement(16803..17294)
                     /locus_tag="PKM27_08825"
     CDS             complement(16803..17294)
                     /locus_tag="PKM27_08825"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNR07405.1"
                     /translation="MKNTLLMNFTVDRENKKIKVEREFAAPLAKVWAAWTDPEILDQW
                     WAPKPWRAETKSMNFKVGGYWLYAMVGPDGTRHWARVDYQAIDPMNYYTAQDAFCDEN
                     GEVNTSFPINVWLNEFKASGESTMVHVTLSFNEVSDLEKIIEMGFKEGFTMAMGNLDE
                     LLA"
     gene            complement(17325..17648)
                     /locus_tag="PKM27_08830"
     CDS             complement(17325..17648)
                     /locus_tag="PKM27_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR07406.1"
                     /translation="MKSKSTRRDVFQAIADPTRRAILALLAMHAMTPNALAEHFDTSR
                     QAVSRHIKVLTECQLVSHEQSGREIYYHFQPKKLKELSDWLEPFRQLWESKFDQLDEV
                     LKKLK"
     gene            complement(17809..18384)
                     /locus_tag="PKM27_08835"
     CDS             complement(17809..18384)
                     /locus_tag="PKM27_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNR07407.1"
                     /translation="MITRYRISGHPIKPTAFILLYIILFSDCRPEPERPLPFYPGKPE
                     IPASILKEHEYLLGRIHQITMFPDSTGVIAKKMEELMQHHFNEEEGYALPPLGLLPSL
                     ANAQWPEQIKEILLLTEKLKSQMVHLNVEHQLINAYMNELKQAAAKDKHSEVWEFEKE
                     LLRHANLEEQVLFPATLLIGEHLKFQQGISH"
     gene            complement(18405..18878)
                     /gene="cdd"
                     /locus_tag="PKM27_08840"
     CDS             complement(18405..18878)
                     /gene="cdd"
                     /locus_tag="PKM27_08840"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNR07408.1"
                     /translation="MNKAKIAKQKSKKTVRPGPAFLLRRARQALRYAYAPYSGFYVGA
                     AVLNKKGRVYPGSNIENASYPLCLCAERVALAHAYMSDPKSPILAIAVLCRNPKIMLA
                     EPGFPCGACRQVISEFEDKQKAPIQIIVGTEEGKIKVFESIKLLLPFSFNSEFLK"
     gene            18891..20156
                     /locus_tag="PKM27_08845"
     CDS             18891..20156
                     /locus_tag="PKM27_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07409.1"
                     /translation="MINKIEIAREQRIAFQAALVARPAGLLVFSILIAHRWSFSEIAS
                     FESLSVFQYLVFFAWLPSWGAIMLRLAGREEYKLQKPFLFSNLVLGGLIFCLVFGVAG
                     LFRQNLMPGLPGHSAWWFKIFILMALYYLQQAGIYLAYLRGRSVRQWILAALVFSSWI
                     YCGIFTYDLSSAVFAQIFFSLGIWLLQVHDFVFDKLRWYAPFWKEVLWYSLYTLAGGA
                     GILLAAYYVQDLYGPGREFNWFRYGTREIPVLPALLAAFGYSYLKTGWGGPGIEFLSG
                     ISRQIKWLILPLALFIVFARPVFAFVFGPRFGVAGDLMSIYLLIYIPRMIYSQVVLQA
                     KDEAPWLFWLGLAELTLMILAALLLVPLLGIESLVWILVGGTVFEKIIQMAVLKYRFA
                     VSPSEYLPLPGFYIWAATLTAAYFVSHWI"
     gene            20158..20997
                     /gene="prmC"
                     /locus_tag="PKM27_08850"
     CDS             20158..20997
                     /gene="prmC"
                     /locus_tag="PKM27_08850"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNR07410.1"
                     /translation="MKVPEYILTESMALLVPVFGNRETKALLRALQEDLFGSGFPEWT
                     AATATRWKAAIQRILGGEPLAYITGVSHFLNLKLTVNPHVLIPRPETEELAVMAIHYI
                     KSIHHAQVLDVGTGSGCLALAIKSSCPNAKVYALDIDSKALETAKANADAYHFPIDFR
                     PFDFLNENGWTSLPDHLDLIISNPPYIGETEKTLMSASTLQYEPPHALFAGTDPLVFY
                     KQIARFGKDHLNPGGRIMVEINEFRAKETEEVFRTAGYPGIELYKDLSGKERILVVSK
                     LAS"
     gene            complement(21042..21359)
                     /locus_tag="PKM27_08855"
     CDS             complement(21042..21359)
                     /locus_tag="PKM27_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07411.1"
                     /translation="MKSRSVKVVVISILLMACGSEELPKEVILLAEQRMQLYRDEQDS
                     LCRAKAIQKAEIAVDSFFLSFTQRFLLDSIPVPPKPIKPYVDTSINLDQARPVKPLWD
                     SLK"
     gene            complement(21340..21720)
                     /locus_tag="PKM27_08860"
     CDS             complement(21340..21720)
                     /locus_tag="PKM27_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07412.1"
                     /translation="MKEVRGSRYGVRGASLSNLSRILNLNLNLNLNLNLNLNLNLILS
                     LILLLTLSSCKQANPRTVKLWVEMSDTMVQRMIKEQQPTMDSLCLKKTDSLFRIAYDS
                     IYSKRLTEIRKLIDSSRYDEIPVR"
     gene            complement(21717..22463)
                     /gene="lptB"
                     /locus_tag="PKM27_08865"
     CDS             complement(21717..22463)
                     /gene="lptB"
                     /locus_tag="PKM27_08865"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNR07413.1"
                     /translation="MDDKIILRSEHLFKRYGQREVVKDVSVNVKQGEIVGLLGPNGAG
                     KTTTFYMVVGFIKPNSGKVFLDSQDITDFPMYLRARRGIGYLPQEPSVFRKLSVEDNI
                     SAVLEMTHLSRKEQEIKLESLIDEFGLHKVRKNKGDSLSGGERRRTEIARALATNPKF
                     ILLDEPFAGIDPIAVEDIQTIVAALKLKNIGILITDHNVQETLSITDRAYLIFEGAIL
                     REGTAEVLAADPLVRKVYLGSSFELRKKQV"
     gene            complement(22467..24083)
                     /locus_tag="PKM27_08870"
     CDS             complement(22467..24083)
                     /locus_tag="PKM27_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNR07414.1"
                     /translation="MRPNPNIYIGLIVISAFWLRCANIGNPEGGPRDLSPPEIDSTES
                     TPNLQRNFDPAASKTDIVLRFNEWVKLEDAFKQVVVSPPLQYAPKIELKGKGVLVRFD
                     PREQLKANTTYTINFGNAIKDITENNPIKDLRYVFSTGEIIDTAYIGGKLADAYTGET
                     KENMIVMLYQNQHDTVVFKERPTYFSRTDKNGHFIIRNIKDTVYKIFALEDANSNYLY
                     DQDKEIIAFLDRTVQAGEDSTTLLLRYYSSFVRPRITEQDAETGGVVKIMANFPYEEM
                     ELKADKAQVRLFPMNYRDTLLVYYLPDTSRDWSLEVHLKGEPVDTVKVNRIRQMLADT
                     FKCMTTRQDIKSVAPGVGLTLNFNHPLGLPDTSRVLLVNDTSRFSEPFQLQSGKLVNE
                     LVFTFNGMEQQTYTLTLLPGAIPDYKGNTTDTLIFKLRGLGAGQTGLLTIECTELDTN
                     QTYVIDLIQDDKALRSSSHTAQTAYTLTYSFLNPGTFSVRITEDRNKNGRWDPGDYKE
                     KVLPEKVLIRKNVSVRANWEIREQISWTNQ"
     gene            complement(24428..25342)
                     /locus_tag="PKM27_08875"
     CDS             complement(24428..25342)
                     /locus_tag="PKM27_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HNR07415.1"
                     /translation="MDWHSSVKGFKAYLKMERGLSVNSVEAYIRDAEKLAGFALENYP
                     KTGPEKISLSHLEAFVQFLDQGGLDERSQARVRSGVRAFYKYLLVEDLVMDDPTELLE
                     GPRLMKKYPDVLSLDEINALLALVDLSTDSGVRNRAMLETLYACGLRVSELINLRLSM
                     LYPDLGFVKVIGKNNKERIVPIGSEALKHIHLYLEGVRRRMPHIAPGHEDILFLNLRG
                     KKCSRIMVFKLIKDLAAKAGIHKKVSPHTFRHSFATHLVERGADLKAVQDMLGHESIT
                     TTEIYTHLDTDYLRETLYRFHPRFKMAH"
     assembly_gap    25831..25930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25980..26053
                     /locus_tag="PKM27_08880"
     tRNA            25980..26053
                     /locus_tag="PKM27_08880"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26014..26016,aa:Met,seq:cat)
     gene            26204..27556
                     /locus_tag="PKM27_08885"
     CDS             26204..27556
                     /locus_tag="PKM27_08885"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR07416.1"
                     /translation="MFKHQINGSFDPWVRVGFIVLLIAIPLSFSINIGSSQLNILSEP
                     LIALFAVALIGLIKGKTFFRGNFIRHPLSILGLIYHGWMITMIPFSSNPWVSLKYTLV
                     NAAHYWVFYFGFYFFVKHTGKNILNWMGSYLLSFMIVILFSWYNYSWYGFRMDAAPVM
                     ALPFYNDHALYGASIALVLPFALLIPQNYLKKPGSLVLTGILMIALFLSSSRAAWLSF
                     GAGGIFISGLKFLRLKFITLTGLIAILSLLTMLNYTSLIRLIGSNREESRKGSWMEHF
                     KSIGNITTDVSNLERLNRYRCALRMWADRPFTGFGPGTYQYAYLPYQKPDEMTRLSVT
                     TPRAPDGGPHPTGRGGGAHSEYLQALSELGLPGFFCWIGLLVNGLYTGMKRYYQEKNR
                     EHQSLLLAILFGLVTYFTHAFFNNFLHSEEIAVLFWPALAALAFLDTEREPGAQIKDP
                     "
     gene            complement(27618..27830)
                     /locus_tag="PKM27_08890"
     CDS             complement(27618..27830)
                     /locus_tag="PKM27_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07417.1"
                     /translation="MIGLRLAKEGRGTLSIHDVTGRTIRSIERDWTRGYHEVWLYRRE
                     IGATGVLYYRFESGFFTDSRKMVLVN"
     gene            28085..28771
                     /locus_tag="PKM27_08895"
     CDS             28085..28771
                     /locus_tag="PKM27_08895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR07418.1"
                     /translation="MGQDVLKSNRYLHFLSKTEAANLITVSEFQNECLVQSVGFPAGK
                     CIPWGLAREDMPAAFPDQRPVDVLGCGSLIPVKNWNLWLEVLAMLVRWNPALKAEIIG
                     DGPERKRLEEQIWKNKLERNISLTGTLPRPLVLKKMSISRVFLHTSGYESFGYVLAEA
                     AANGCHVVSTPVGIARDLASCANTAEALFNHVARLLVLPPPAGKVPVKTMEEVAAEYL
                     DLYKNCKKAD"
     gene            complement(28777..29853)
                     /locus_tag="PKM27_08900"
     CDS             complement(28777..29853)
                     /locus_tag="PKM27_08900"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNR07419.1"
                     /translation="MQDKKGPDFFILGLPRSGTTLLEQMLDAHSEIAVCPEMSTGKTL
                     WRLNAESTLRDQWKSLLLVNALYQRARTFKDPILPCLAHQALRSFTYPISTGEWFSPL
                     IREYVGEKKANLYGEKTPENTFFIPALNRAFPGSKYLLILRNPFDLVLSLCEAWAFHK
                     KIRITDALLLHFAENTKRGLHELFIRQRLRNLDSLTITYEDLVRKPDQTLTEICTFLG
                     LEFEPGMKDFQFRKEYTGGKEAMKFLLGRLDEPLTDQRIDRSLDILSAEQIGMLHRYL
                     SPEIHALPYTFPNIQVRIPLFLLIRVYWAKLKFFLRLFYLEELKNKLRFQVHYYAIRL
                     LSEGPIKNYFYKNLKHRPSDWVVS"
     gene            complement(29881..30423)
                     /gene="cysC"
                     /locus_tag="PKM27_08905"
     CDS             complement(29881..30423)
                     /gene="cysC"
                     /locus_tag="PKM27_08905"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNR07420.1"
                     /translation="MLLLQFTGLSGSGKTTLANRVKLLLIDLGFPCEVLDGDEYRGTL
                     CRDLGFTREDRKENNRRLGLLGLLLLKHGILVMIATINPYEEDRRALHSQSNKIFTIW
                     LDCKVELLVRRDPKGLYRRAFLTPGQPERIQNLTGINDPYEPPLHADLVIHTDRESPE
                     ESAEKLLHFILEKIGHDTAS"
     gene            30515..31843
                     /gene="eat"
                     /locus_tag="PKM27_08910"
     CDS             30515..31843
                     /gene="eat"
                     /locus_tag="PKM27_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HNR07421.1"
                     /translation="MQTPSEASLKKALSPLMLWGLGVGYVISGMYFGWNLGLKEGGTL
                     GLAIATFFIIILYTTFSFSYAELACSIPKAGGVFDYANRALGKNWGFLAGMAQIIEFV
                     FAPPAIAYGIGAYFNLFFPGIPVLAIAITAYVLFTGLNIYGVKAAASFELIITLIAVV
                     ELLIFVGVALPHFEAANLNHNAFPNGWGGVFAALPFAIWFFLGIEGLANVAEETKNPQ
                     QDILKGFGSAILTLVILCILIFLSATGVGGWEQIVYKDGVGGETSDSPLPLAMAQVVG
                     SDSVLFHMLLTIGLFGLVASFHGLMLAAGRSTFEFGRVGFAPGFLGRVHPRFRTPANA
                     LLVNMVLGILALLTNKTGEIITVSVLGALTLYVLAMISLLVLRRKEPDEPRPFKVPGY
                     PWTPGIALVISLLALVLVNYYNLKLTLYFLVLMLLPYFFFKLFYKKTETV"
     gene            31840..33195
                     /locus_tag="PKM27_08915"
     CDS             31840..33195
                     /locus_tag="PKM27_08915"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNR07422.1"
                     /translation="MSEQEVFNYVKNHPDQKIKLAIADTDGILRGKYISTEKFLAAAA
                     GHLGMCNVVFGWDAGDAAYDNARYTGWHSGYPDVPARIDLSTFRKIPWENGIPFFLCE
                     LVDKKESPAYVCPRNLLKRVLAEAEEIGYKPIFSQEFEWYNFEETPHSASDKKFSGLK
                     PLTPGMFGYSILRTSLRNDFFSALFDELKKFDLPLEGLHTETGPGVYEAAIAYSGILE
                     AADRAVLFKTAVKELAYKMGIMATFIAKWDENLPGCSGHVHQSLWDQEGKNNLFHSER
                     DKTGMSELFKQYMAGQLFCLPHILPMYAPTVNSYKRLVEGAWAPTTLTWAIDNRTTAL
                     RALPGNKKSCRLETRVVGSDVNPFLAMAACLASGLYGIKNKLKLQAPTKGNGYRDTLH
                     GVLPSNLWIASQTMKESPLAKELFGPEFVDHFTATREWEWRQFSRVVTDWEKRRYFEI
                     I"
     gene            33772..34920
                     /locus_tag="PKM27_08920"
     CDS             33772..34920
                     /locus_tag="PKM27_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNR07423.1"
                     /translation="MDFNQIRQFNFPTLIRFGAGAAQELAPYLKDNQLSRPLVVTDPQ
                     VAQLPFFTKVIRELENNGLSVEIFSSIHKNPVKSDVYLGTDLWDSSDRDCVVGIGGGA
                     ALDVARAIVLRVHHREDLFKYDDLVGGDVYVTGEVPHFVTLPTTAGTGSEVGRSAIIS
                     DDLSHQKKILFSPKLMARRVFADPLLTMDLPSAITAATGMDALTHNMEAFLAKNYHPI
                     CEGIALEGMRLISLSLEKAVNGPNLESRAQMLLASMMGAIAFQKGLGVVHSLAHPLSS
                     LLDTHHGLANAVNIPYGMKFNIQGSEDKFSRMAQALGLKTWSGKAVVDFLIQLNEKIG
                     IPARLKDIGVQQQHLDPLADLAIADFAHPNNPKPVSRQDFLALYEEAL"
     gene            34975..35706
                     /locus_tag="PKM27_08925"
     CDS             34975..35706
                     /locus_tag="PKM27_08925"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNR07424.1"
                     /translation="MITIGITETDTNFKHYLPWIQGGDPDIAALILSYKSPNLDDLGK
                     CHGIVLSGGVDWHPKFYNNPRTDYPRADHFIEERDEFELAVFKYARQYRLPVLAICRG
                     MQLVNIALGGDLIQDLEESGKKNHRRMNGEDGLHQVKVRPGSLLHDIVHREEGMVNSA
                     HHQALGRLAEELCSSASSEDDLAEAVEYKSKKDQGFLLGVQWHPERLDIRKGSPAFSK
                     NIRDAFVRAVREKQESSHHPKNSLQ"
     gene            35703..37079
                     /locus_tag="PKM27_08930"
     CDS             35703..37079
                     /locus_tag="PKM27_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNR07425.1"
                     /translation="MNIINPATEEIIATVKEDDSFSLEKKLALLKKGQKTWQAVPLEE
                     RIKRIDRFAGLLKENIESLASTLTSEMGKPLQQSRNEINGASNRIGWLTGHAGQFLSE
                     ELMNRSGDMTEKIVYEPLGVVCNISAWNYPYNVGVNVFVPALLAGNAVLYKPSEYATL
                     TGLGIARYLQEAGIPEEVFQLALGSGEVGKLLLDLPFDGYFFTGSYKTGKYIYEKVAP
                     KMVPCQLELGGKDPVYAADDIEDIKNSAAGISDGAFYNNGQSCCAVERIYVHEKIYEA
                     FIQSFLDEVMSWKIGAPDEAGTYISVLSRKAQLEVLDRQVADAVSKGAKVLAGGKRLD
                     RKGYYYEPTVLIEVDHSMELMKEESFGPVIGIMKVKNDEEATHLMQDTEYGLTASVYS
                     SSWDRAQKILSRLDTGTGYWNCCDRVSAAVPWSGRKHSGIGSTLSHAGLRAFTKPKAY
                     QLRATGSE"
BASE COUNT         8623 a         9006 c         9698 g         9719 t
ORIGIN      
        1 ccggtataca ccttccttac ggattatata gcccatggag tggatgtgcc tgaagatatc
       61 gggcggatag aggagatgat gggaaggcag taggtggtgg atggtggatg gtggatggtg
      121 gatggaattg aatcaatcat aaatgatcaa tttcccggtc cccgctttat taaaaccata
      181 ttcgatccgg taccaataga ggccttgagg ttggctgcgg agatccagtt cgttttgctc
      241 cacggtcaag gtccgggcat agacgcggtg gccgaggctg ttgaaaatgg caatggatac
      301 cgggggcctg ccgtgattta taagaaaaaa tttgcccgtg gatggattcg ggaaaacctg
      361 aactgccgat aatccgggat ctttgccggt agaagtgctt aacgtatggt aggtgatagt
      421 cagcattggc cggttcgttg cggatagaga ctcacggctt gcaaattgtt tggcggtggt
      481 agaactgact tcattcccta caatacacca gccaaaattg acggaaggag tgtccagcca
      541 tcgctgggca tcctttatca tttgagcgct tgaccagctg taaatgccgt ccccatcaac
      601 ctgtatgctt gctgaggcat tgccgatgaa atcgcccccg ggttttgacc aggggctgcc
      661 cggccatagg gcatggaccc aagtggcatc tccgttctga gcagccgccc caccgccttc
      721 ttcgccgtcg gccatggaaa ttccctctcc ccactccctg gtcaaaccat gcagactaac
      781 cggttgaggg cctgttatgg tccggtccat ggtcaaactc aagcgaaccc cactaagggt
      841 ggccccgggg ggcacttgtc cggcaatatc aaatttcaca agtccgcgtc ggatcaaccc
      901 gtttttggtt ttgccagcaa aaatttttgt ccctttccca ttggatatat ttcccgaact
      961 gctttcatac aaggtgttgt ccgccgacgg cgccagagtc agggtggtct gtgccccggc
     1021 aaaccaagtg aacacaaaaa gttgcactaa aaggaagaaa cggaaagcct gtttgttcat
     1081 aacggaattt cgttttttgg gttaatgcta atggtaaagt ccccagatca gcttgtcgat
     1141 tttccaatcg ccctttttat tccggaccat taaggcaatg tacttatcat ccacccggta
     1201 ttggacggag tcatcgagcg aagttctaaa tcctttattg atgccggtga ccagcgcatg
     1261 atcggaaaac agctgcgttt caagaatttg ggtttggagg ttcattttat aagccttcag
     1321 gagagtttca taattctttt ttacggttga aattccttgt atgaaagctt tcccagccgg
     1381 ataaaattca atgctgtcgg aataggcatt cagtatggtt tccaggtctg atcggttgtc
     1441 ggcgtcaata agccggtaca agaccgcatg aattttttca atttcatagc ttgtattttt
     1501 tgcgcaaccg gttaaaaaac ccgccataag cataaacaag acagccggag agatcatggt
     1561 ccgcatatcc ttactttgag gtccgattct ataacgtctg gtccggatct tgcgcggcta
     1621 agatgaaaat gcacctggaa atagccgccg gttcattctt ctgaatccaa tgacctggtt
     1681 tactcccggg actccccgga tcaaaggggc cggaccatcc tgtacaagtc aaagccctcc
     1741 gcccaataat cttttatcac ttctttgagt tcgaatccga atttttcata aaaacgataa
     1801 acccattggg aagtcctcac tgtaatggtc tttaaacccg gaacctcgtt tatccggtca
     1861 atcctgtatt gaagcagggc tgaccccaag ccccgtccct ggaagtcggg atggaaaaga
     1921 tcccagctga tcctcgcatc ggcttgttcc gggaataaat tataaccccc gcaaccgaca
     1981 acggccccgc cgctttcaac aataaaaaag tgcccggcat gattttccag gtaatctata
     2041 aagtggcttt cttcagtaat tgaaaaatac cgcggggtgt tcaaacggaa taattgaagg
     2101 agaaccgccc ggtgggacgg agtatatttc cggatggtga ttttagcatc catgtcatgg
     2161 ttttccattt tatttagaaa gtggaagatc gtcaggaatc caattcaacg gatttcattt
     2221 ccgccctgtc gattatcata aacaaaccgg tccctggccg gcattttaac ggcgggcagg
     2281 gtcaaccggt tcatttcagc atcctgagga atgatttcgt tcaagtgaag caaataggca
     2341 gtgatcgcat acatctcatg gtcactgagg gaacccggct cattttgggg catggcgcgc
     2401 cggacataat cgaaaagcgt ggtggcatac ggccaataat tcccgatcgt tctcatcgag
     2461 aggtaccggt attccctgcc gaacgggaac ccttccctgg gctccctgcc caccaagcgg
     2521 tcaaatgggc cttccgtacc ggtagctccg tggcaaagaa tacatttttg agcatatacg
     2581 agccttcctt cttcaacttt gccttggcct tccgggagcc cggtcccatc tggcatgata
     2641 tcgatattcc atgcttcgat ctccgtttcg gaaaccgcat ggccgaatcc aaaagattcc
     2701 ggccagtgat ccgggaaggc cggccctgag ccggcggcct gcgcagaaat tccggagtcg
     2761 ggaagattca gtgacccact gcctgcttcg ggtccgacgg cttgcttgca ggcaagccat
     2821 ccggacaggc agaccaaaag gatcttaagt ccaggttgaa tggcaggatg aaaaaatgcg
     2881 accttcccct tataccattt cattcaatcc gagttttaaa cttccgtcct tttccacctt
     2941 ccaggcgcgg atatgattaa agtggtagaa ggtgcctttt ccgcggactt tttccaactg
     3001 gccgagggtg ggttgctggt aaccggtttc atccatcgcc ctgctcatca ggatggccgt
     3061 tcctcccttc caattccaca ggtagcggaa gcgggtctgg cattttgaaa gaacgggctc
     3121 ctggagttca gccggaaacc aatgttttcc gccatccgta ctgacttcca cccggatgat
     3181 tttgcccctc ccgctccagg catatccctg gatctcccac cagcctttat caggcagaat
     3241 atagggatag gatggaaaag taatgaggga tttggcatcc atcacaaaac taaacaggcg
     3301 tgctgtgcaa tccgggaggg gatccgaata tttggaagtt tcttcacggg tcatgaaggg
     3361 ttgatctgaa acttcaattc ttctcagcca tttcacctgg gtattgccct cataaccggg
     3421 taacagcaag cgggcagggt aaccctgttc cggccggatc gcttccccat tttgcccata
     3481 tacgatcatt gcatcttccc atcctttttc catagggatg cttcgggtca taaccgccgc
     3541 atctgaactt tcggcgagaa accacttcgc acccggtttt atacccactt cgcggaataa
     3601 ggtggacagc atgaccccgg tccattcgct ggtgctgatc agtccatcca tgcgctgagg
     3661 cgtcaccgct tctgtaatgt cttttttgaa ataggtcgac cacccattgc cggaacactc
     3721 aataaaacaa atgcgggaat gttgtggaaa acgcttcagg tctttcaggg taaatttttt
     3781 cggggtttta atcaaaccgt gaatcagcag ttcataggat tcggggtcaa tggatggtat
     3841 ccctccatga tgcctttcaa aatgcagatc agccggcgta ataataccgg ataagttctg
     3901 gatcggggtc atcgattccc ccgaagggct tggcctcaag accagccttt ccagatgttc
     3961 atccggtgag cggcttccaa gttttgaaga tccgcggcca ggaatcctgg tcggatcgtt
     4021 ggcttttgaa ttggccgggg tagtggatac ctgtgcctct gcagcggtac gcacgaacgc
     4081 cccgcctagg gcagctgcag atagtttgag gagataccgc cttgaaaaaa aagatccttt
     4141 tgttgatttt tgtttcatca taccctgatc aggtaaatca ggccctggat gaaattaatt
     4201 aaaaatttta ttatactgta gccccggagt gatcggggag caaatcaaag gggcgcggga
     4261 tacccgctgc tggccggtaa tgctctgtta aaaatccgta ttgggttttt tcaacttcag
     4321 ctccttgatc cagatattcc ggtaaaggac atcgtgccct tcagcctgga gtttcaggcc
     4381 ttccggcgta tcggtaatgc ctttgccccc gtcattacct ccatccacac ctgaattggg
     4441 ccctccccaa acctggttga tggtctgatt ggtatgcact tttttgccgt tgaaatacag
     4501 ggtcaccatg gctttttcgg tacgctttcc atccttgaac cgggctgccc ggaagacgag
     4561 gtcgtaggag ttccacttgc cgggtccgtg gtaagcgtaa taaggggatt cggtttcatt
     4621 gatcacggcg cccaggccgt gtttggtact gtccccgtcc aggatctgga tttcataccg
     4681 gttttgcaga tagacgccgc tgttgcctcc cggtttcata atcaggaatt ccaggtgcag
     4741 tcgaaagtcg cggaatttct tttttgtgac gatgtctgcc gaaccgtatt tccctcctgc
     4801 cgccgagggg tcattgctgt tgacggccgt acccggatcg acgggatcct tgacgatcgc
     4861 ccatttcaga gggagtgtgg cggacagcct ggggcctgcc cagtaggtcc atttttgatc
     4921 cagcatttcc cggctgccgt cgaataaaac ttcagcccct ttcgccggct tagcgccaac
     4981 acccatctgg gcggagcctg taaaaggcaa cagggtgagc aggaacaagg ccccaaaggc
     5041 cttcgtgagg gaagaaatgg tatgtgcagg catttttttc attttttagg caataatagc
     5101 aagttagtaa gttagtaagt tagtaagtta gcggttgatg gcaatataaa gcaatacggg
     5161 caggtcgtga acgcttgggc gttattggaa gttaaaaatg accacggtcc attacgaaat
     5221 tccggcactc ggacatttcc gggatcttgg gatgaggttt attttttata attgaaccgc
     5281 agcagggtcg cttttttatc ctggccttcg ttgctgatca gcaaatcccc attgtcataa
     5341 aaagccaatc cctcggcctg gttaaacaat gcaggatcga ggagttccag gtgttccagg
     5401 gtccagtcag cggaaaaaat aaacaacata taatccggag cggagagcag gaaaaattgc
     5461 cgggtcaggg ggtgaatgcc tatggccgta ggcctcaatt ttatatccgg ttctttttcc
     5521 ccggattttt tttctttcat cggtattccg ctgacttttt ccagggcgaa ttttttgatc
     5581 acccgttcat cgaaacttac tacggctggt tcaaccaggg atttggtttc caggtcgaag
     5641 gcataaaggt ctcgcctgtt tttaagttct tctccttttc ctgaattgcc tttgctggcg
     5701 atcagcaatc tgcccttacc ggcatcataa cagaggcctt cgttgtcata agcggggata
     5761 ttgagtccat aggagatcgc cttggccggt tcaccggaga aatccgcgat ctcgaaaagg
     5821 gtggcatcgc ttcgtaagat atacatggcg ttcccaaatt tcgcgatatc ctcaaaatca
     5881 ccctgcccgt aaaaggaata ttgatttatg atctggccgc tggtgctgtt gaatatatag
     5941 acgatgcctt tttcatcctg aatgcaggcc agggtggttg aatccatcac ggcaatgccg
     6001 gagatttcat gaagggaggc gggaagttcg aggacctggt cgggttttgc aaaatgatag
     6061 cctgtaacct gttcactgct cttgtaaacc cgagctgttt tatccagcct ggcctggcag
     6121 ttaaataaag ccgccaggat tagcaggcct atgctgtaaa agggatgaaa gacatggcct
     6181 gatatcataa gtttaaagct atgagtaagt gtatgggccc aaaagcaggt agacaaaaat
     6241 aaccaaatcg atatatccgt caaaacggct catgccgcct ttgcggtgat tcaagacggg
     6301 gcgggaggtt cattcctcat ggcctgatcc ggggcaggca tcatccggca agtctttccg
     6361 aaacctcacc ggtagctgta tcatatatga gcaagccttc ccgggcttca ggaagttgtc
     6421 ccaccatgag gccttcttta ttccagacgg acgaacgacc tccggctaaa aactggtcgc
     6481 agatgccaat gcaattggac atcatgaccg ggatggaata ttgaagggca atttcgggga
     6541 gccgttttac agcatggtct actccctgtc ttgttttagc cacgctggcc atataaacgg
     6601 aggcgccgtt ttgcataacc cgggcggcat gttccggtat ggagatttca taacaaatgg
     6661 ccggtgcgat cctgagattg ccggtggaca aaaaaagttg ccctttgccc ggaataaaat
     6721 aaggttcttc atcctggtgt aagtattgtt tgcgataaac ttgcctggga ctatgagctt
     6781 gaaaaaaaat ctggctgata taggtcccat gtgtaccctt cagcggcatg cccagaccga
     6841 gggtgacctg gtgcaagtca ctcacttgtt gaaagacatc gaggcacgga tcatctggat
     6901 ccatggcaag gtcctgagcc aagccgggtt catatccggt aagggacaat tcggggaaaa
     6961 ggatcatgcc ggtcccatgt tcaagggccc gttcggtcca tgccaaatga ttattgatat
     7021 tttggcggat atccgccttg accggattcg tttgggcaac acaaattttc atatattcag
     7081 gatatggggg attgaccgga attttgacct ggacccctcc ttgagaaacg gataatcaaa
     7141 tatccaataa cgctcgccag caatgaagcg aacaagatgc ccattttggc ctgttccacc
     7201 agggcagggt cttcgaaggc aagattggaa atgaacaggg acatggtgaa cccgatggcg
     7261 gccaggaatc ctgctcctaa taaatgccgg gagttcattc cgggaggcag ctgtatccat
     7321 ttcagcctga tcaggaccga agtgattccg aatacgccca gcacttttcc ggccagcaaa
     7381 ccggtgaaca cgcccaggaa gaccgggttc actacctgga ggagggcttc tgcagaaaga
     7441 accacgctcg cattgctcaa ggcaaacaga gggatgatga taaacgagac taaaggatgc
     7501 atactgtgtt ccagccgctg aagcggggtg agcgccgcta tggaaaattg ccggatatca
     7561 tccaggatga gcaattgatt ttcggtaacc gttggtttgt tattgggctt ttccgcttca
     7621 aaccgggtca tcaacctttt caggccgtag gtgtattccg cttcggagac ttttactttg
     7681 gcaggaatcg tcatggctgc catgaccgcg gcaatggtgg catggacccc cgacatcagg
     7741 aaagccagcc ataaaccacc gatgcccaca atcccataaa aaagggtgtt ccgcaccccg
     7801 atcagattgg agatgatgag gacagccata aaaactcctc cggttgccag gcttgtaaaa
     7861 ttgatatctg aagtataaaa aaaggcgatc accagtaccg cgccgaggtc atcgacaata
     7921 gccagggcgg taagaaatat tttcagggat aggggtaccc ggtctcccag gaggtagagc
     7981 acacccaggg cgaaagcgat atccgttgcc atcggaatgc cccaaccgga tgccgtttcc
     8041 gaagaaatat taaaatacaa atagatcagg gcagggagag ccattccgcc tatggctgcg
     8101 aataccggta ccagggcttt tttcagctca ttgagctccc cggcgacaat ttcccgcttc
     8161 aattccaggc cgaccacgaa aaaaaatacg gccattagtc catcgttgat ccagtgatgt
     8221 aaacttttgg aaatgatatt tccgtcgtag cctatggaaa attcatgttc ccataaatgg
     8281 tgaaattcat ccgaccaggg tgaattggcc aataggagag cgaggagcgc cgacgaaaaa
     8341 aggacgaggc cgctcacggt gctgttgttt ataaatcttt gaatgggatc gaggatccag
     8401 cgttccaccg gtgttttacc ggttaaatgg gtcttttcgt cattcatcat tttttcattt
     8461 attcccggcc tggtcggggc attcagggct gcgggaccgg aatgatccca atcctatcgg
     8521 atatgagtcc ctggatggcg aacgctggtc gggcatgccg tttttttccc ttcgaatccg
     8581 ggctcgatga ccggttaaaa ggatttcaaa gttcatgttt ttccggcatg ctttgccaga
     8641 tctcaaacat cacttcggcc ggttgaagcc cgtgcgggtc atatcgcccc agcctttgct
     8701 gcctctccat ttttcccagt tgccccgcaa tgccgcataa acggtaaaag gatgaaatat
     8761 aaaaggttcc agcagggcga tgaatgccag cttgcgcagg tccctgaacg tattgtattt
     8821 cttaaatgtg gattcctcgg caaaaatggc aatggccgag aacatcaagg caaaggagta
     8881 gaccatgaat aaaaacagga agaaaaattt ccagctgagg atgccaaaga tgatcaggat
     8941 gagggaaatg aggatgccgg taaactcaat gatcggggcg agatattcgt agatcagcca
     9001 ataagggtaa ctgagcaggc ccaggatttt atatttaggg ttgaacagca ttttacggtg
     9061 aatccacaag gtctccatgg taccccgggt ccaccggctg cgttgttttc gcaatatttt
     9121 gtaggattcg ggagcttcgg tccaacacag ggggtcggga aggtattgca ccctgtagtc
     9181 cagtttatgt tcgatcatat aacggcgcat ccggaccacc agttccatgt cctcgcctac
     9241 ggttttggta ttgtatccgc ctgcccggag cacgatgtcc cggtcaaaca tgccgaaggc
     9301 gccgctgatg atgagcagcc cgtccattct ggcccaggcc atccgtccaa ggataaaggc
     9361 tctgaggtat tccagtacct gtatgcgggc gagccagttg tcgggggcgt tgaccttgac
     9421 catcctgccg gacttgatca cgcaggaatt ggcaatcctg accacgcctc cggtggcaat
     9481 gatccggctt ccggattctt ccataaaggg tttggccagt ttgagcagga cgtctttgtc
     9541 catgatgcaa tccacgtcga tgcaaacgat ataaggatgg gatgagatat tgattcccgt
     9601 attcaaagcg tccgccttac ccccgttttc cttatccacc acgatgagtt tggaatagga
     9661 aggattggat gaacggtaga tcgcccttac cggttttgtc gggacctgtg gatggtaggg
     9721 caggtcggag gggatcagtt caaaggcttc gaccaggatg gggatggagt cgtccttgct
     9781 cccgtcgttt accacgatca catcaaaatt gttgtaattg atggacaaca gcgattgcac
     9841 attttcccgg atggtcttgc cttcattgta ggccggggcg atcagggata ctttgggcgt
     9901 atggggcagg gccaaaaggg ccccgtaatt gatgtaccgg ttcttttgga ggtaggcttt
     9961 caatgaaaag gaagagatga tggccaggat caggtaagag agcatcacca ggatggcaaa
    10021 ggccagtata aggtaattaa atatgccgat cccggtatcc aaccatccca tccccgtcat
    10081 atcgaagtcc gctcctgcgt ttttaatagt gaagcaatat cgtccagttc ctgtttgagt
    10141 tttgggttgc tgcttatgtt cattgctttt tcaatggccg gaatataccg ggtgagttga
    10201 tagtgttcga tcagccgcgc cccgaggagg accatggaat cgttggggct ggacaataag
    10261 gtgtcgaggt ggtcaatggc cgggatctgg tgggatttca gggcaaacag gatattgctt
    10321 tgcatccagt cgtccaggaa caactgccgg tttttgcgtt tcaacaattc cgccaggccc
    10381 cggagccgga tcaggcccag aaatgcttct ttctggacaa attcatgcgg gtggtaggtc
    10441 agttgctcga tggctttgat ggccgggtag tagttgaggt tggtgaggtc ccgcagtcct
    10501 tccaccactt ccggccagga cctgtgtttg agtttgctga gcgagtaatt ggataggccc
    10561 gattcgaaat agaggtcctc cagtttttga cggaaagtcc cgctgtaatt gtgatgcaag
    10621 gtgacaatgg ttttgatggc cattttttta aacaaaaggt tgcctgccag gccctgcctg
    10681 aattttaaaa gggcctccgg ggcaggggac tcgttgaaca ggattgaaaa aagagtgttt
    10741 tcaatggtct ccgtaaattg gatttttctc cgggccttga ggattctctg atatcttttg
    10801 ataaaggtcc aggccaggaa aaaaagggcg gtcagcgcga aggccaggcc cagcagcaga
    10861 atccattccg cctgggagat ccggccatca taggatatgt ttgccgaagg gaacactcaa
    10921 aaccgcttaa ggatgatcca ctgaaaatta aaccggttgc ggtacgtgtc cgggatatat
    10981 tcttcccgtt catacatgat caccggctga atgaaaacat gattgcccat acggaacatg
    11041 ccgttgatcc cggcacgcca ggcgctgatg cgggaaaagg catcgctggc aaaaaaataa
    11101 tagggggtgc cgccatattg caggtccagc tgaatatagg actcccggcg ctccagagat
    11161 ctgcgcaggt tgaggacatg agaaacaaac cattcggact ccaggtgcag gagataattg
    11221 cggtagccga atttccactc gccgaaattt atggcagctg accctgtcag caattggacc
    11281 ttctgaacgc ggaagtcgag atacctgccc ccgattgaaa aattgtaccg gttgatatcc
    11341 tggtaatact cccctgccaa acggtacctg gggaaaatcc ggtcagcccc ggaatatccg
    11401 gcattcaaat agaggtatcc gcctttcccc acccgggggt aggcatccgc ttcgacctga
    11461 accgcgtccg acccgaacaa atgaccgtag ttcaagcggc ctccaagagc gatggaccgg
    11521 ttcctgtgaa aatattccag gtgggccagg tgccaggggg cgaatccggg attgtaaaaa
    11581 ctggtattga gatatccggc tgcaagcgta tttttttctt tccggaggat ctgggttttg
    11641 agatactggg catcccggtg attgttcgcc gacttcggga tttggtccag caaacgaacg
    11701 gcctcccggt cttcccccgt tttctccaaa gccatggcct cctggaaatg ataaaaggga
    11761 atcccggcag gaaaaagcga atggcctttc cggcactttt caatcaccac cggataatgt
    11821 tcgagttgat attcaacctg gattgccaga tcataggctt ccgcgaggac gggctcggaa
    11881 atcgtaaggg gcatcaggtg ttccagggcc cggggataat cctttgacca ataataaagg
    11941 cgggccaggt atagtttgta gtccaggttt tccggatggt cttcattcaa tttggtgacc
    12001 agtgaaatcg cagtcttata atcctccccg gccgccagct tttgggccag ggccatgact
    12061 gaatccgggt cctgcctctg ggcggaaagg gccgcacaga atatcaaacc accaatcagg
    12121 ccagcccagc agaaaaggat ccgttttttc atgggattta aatcaggtgg aagtcaattt
    12181 ttctatcctc ttcagcagta ctgcaggact gaagggtttg gaaataaaat catccgcccc
    12241 gagctcaaag acttccaact ccgtactctc cactccggag gaagtgagca cgatgatctg
    12301 gatatcggca tccttgatct ggcgggtggc gtgtatgacc tgccggcctc ctgcaaacgg
    12361 aaggttgaga tcagtgacaa tgatgctgat gtcggcgtaa tttttccgga tgtaatcgat
    12421 gggatccagg ccattttcaa attccaccac ttcatgacct tttgatttta aaaaaaacga
    12481 taacgattta cggagcattt catcatcctc gatcaatacc actttcatgc tttgaagtta
    12541 tttaatattt tttccacttc aaaatgaagc tgacggagtt gattttcgag ggcgctcagg
    12601 accggcataa tctcatccgg agctggcttc cggctggcca aagtttccag gttttcgagc
    12661 agcaggaggt ccgcaccgac catgccaaag gaggatttca atttatgggc catggctccc
    12721 aggttgatca ggtcgccccc cgccagataa gtcgtcatat ctctcaaggc cttttcagat
    12781 tgattccgga acagcaggag catttctttt tgaaagtcgt tgtcgcctcc agaaaatgct
    12841 tcaagttgtt ccagcgagat caatggttcc tctttgccgc cttcaagaac cggaggttcc
    12901 tttaattcct tcgagtggtt aagccaaaat tcaattttcg ccaggagggc ttctttctta
    12961 aacggttttg aaaggtagtc gttcataccc agggacaggc atttctccct ttcagccacc
    13021 atgctgtgcg cggtgagggc aatgacgggg accgttgatt ttaagtccgt gcgaatggcc
    13081 cggatggcct ggtatccgtc catttccggc atctggatgt ccatgagcac gaggtcgtag
    13141 gcctttgatg ccagcaggtt taatccttcg ataccgttga cggccaggtc caactggtaa
    13201 ggggtgttcc ggaaaaggga ttgcaccagc cgctgattca attcgttgtc ttcacacatc
    13261 aggatgcggg cggatgactt gtttggccgg tcgctgtccc ggagaggggc cgcagcgaag
    13321 gattcgttga aatatatttt acagggaaga ctgaactcga agcgggaacc cttgcccggt
    13381 tcgctttcaa cccggatcca tcccccctgt ttttcaatca gcaaccggct gatgtttaaa
    13441 cccaggccgg tacctccgta tttcctggaa gtatttgctt cggcctgggt gaatcgttca
    13501 aagataaggt ccagtttttc ttttgaaatg ccaataccgg tgtccgaaat ggtgaagtgc
    13561 agattgcttg gttctcccgt tggaggttca ttcctgaggt ccaggtgcac agcaccatga
    13621 tgggtaaatt tgatcgcatt gcccaataaa ttgatcagga tctggataag cctgttttta
    13681 tcacccacaa ccatctcggg taggtttgga ccgaaatggt aatcaaattg cagacctttc
    13741 tgggcagcct tcagccggag catgccgaat acgtccctga cggcttcttc cagacggtag
    13801 ggaaggtgct cgatctggag aaggcccgct tcgatttttg acaggtcgag aaggtcattg
    13861 accaggtata agagggtatc gcccgccagt ttgatggctt cgagttttga actgaactct
    13921 tcgggcaggt ttttttccag caaaaggtcg gtaaaaccga tgatggcatg gatcggggtc
    13981 cggatttcat ggctcatgtt ggcaataaaa gtgtcctttt gcgcattcag gtcgtcggca
    14041 attttttttg ctttttccag agccaggcgg taatgggttt ccatcgtgat gtcctgggcg
    14101 attttggtga tccggatcac ctgttcatgg ctgtcgagga ccgggctgta ggaagcctga
    14161 atgtaaagga ctgaaccgtc cttccgaagg cgtttaaaac ggccggattg aaaagctcca
    14221 cttttaaggg attgccagaa aaggaggttt ttcgcctggt cttcctggtc gagcagaacg
    14281 gtatgatgct tgcccacaag ttcttgttca tgataaccgg tcatctcgct gaaatttttg
    14341 tttacgctca ggatgaagcc ctccggggag aattccacga cgccgttgga ccgggtgatg
    14401 gcctggaaca atttgttgta ttccgcttcc ttgaggttaa ggagctcctg agtctcttcg
    14461 attttctttt tgagcacatt ttccagttcc cggccaaggc gggtattttt ttttcgggtt
    14521 tcgaggaggt ttatcgtatg agaggccagg atttccaggg cttttttttg cggctcatcc
    14581 aggaggcggg gtttatgatc gatcacgcag acggtaccga tgggaaggcc cttttcggtc
    14641 ttcagggcaa tgcccgcata ataccggacc ccgttttctt tggtcacaaa agaattggta
    14701 cacacccggt cgtcctggct tgcatccggg atttccagta aagtatcttg acggatggta
    14761 tgcctgcaaa aagtatcttc gatgggaact tgcaggaggt ccatgcccac ttttgcctta
    14821 taccattgcc ggctgtcgtc gataaaggag atgagggccg tcggggtatc gcagatgatg
    14881 gaagccagtt gagccaggtc atcaaactct ttttccggag aggtatccag tattccgaaa
    14941 gacaagagtt cctttaacct ttctatttcc aattgttttt cgcctttccc gccaatatcc
    15001 atcccgcctg ttttaagtaa aattagtcga ttccggaatg atcccttcgt ttgcttaaaa
    15061 aatcacagtg gccgagggtg gtcagcccga tccggaaata tccggagccc catgtagtcc
    15121 ctttattatg gaaccattgc taaagaatta agctaaacgg tcgtcgccgg ggatggtgct
    15181 tcccggtgtt acacttcagg tcttctttgc tgcccgcttt ttccggactg aattcgaatt
    15241 ttcctccagg cgaaaccgga taattttttg gatcaggcgg atgggcaggg ccttgttcag
    15301 gggaaagcgg atggttcctt tgcccccggc gaaagcggag atctcctcct taaatgcggg
    15361 gttattccgc ggggcggggt agaggctgac aaaatcccgg tagccgccga aataaatcag
    15421 gtaatgacca ttcaggttga aggccggcat gccataactg agggtttcct cagcctcagg
    15481 aaccagggtc ttgaccaggt ccctgatttg caacatgtgt ttccgggctt tgaccggaag
    15541 actggaaata taatccttca tggatttagg tttttcagtt ttcatgttga ttgaagtttg
    15601 atcattgctg ttcttccccg gtattgaaat ggaaccagtt acaaggtaaa ggcctcttct
    15661 aagttccggc ttttatctgg aaatttacat accggtgcaa agaatcagat atcgatacgg
    15721 tgattatttg aacttaccct accggggatt ataatttccc atccagccag tgtatgatat
    15781 tgagcaataa ttgatggttt tgagccgcct ccgggtgatt catgcccacc ttgacggata
    15841 gggacccggc cagttgggcg gtaaacatgg cagcctcgcc ggctacaacc accctgcctt
    15901 gcccaaaggt cctgtaagcg cactgggacc agcctttaat gtttgatctt ggggtcgttt
    15961 cattaaatac ccaggcggta tccggcagaa aattttcaaa gttttcccca aaggttaaga
    16021 tggaaaccgc ttcccggggg agccggaatc cctggcctgt gaaagatacg atttgactga
    16081 ccttttccct ttcgttcctt cccatggtga ctatattcac ggccagcgtg gtatcctggt
    16141 tcttaaaata tgcagtccca tcggttacgg tgttcagcac aaatccattg ctgaattcaa
    16201 atccaaaggc cgcagccagg tctttggctg ctcctgccat gggcatatga tctgctatga
    16261 ggaataagtt tccgccctcc tggacccagt tttttaccag gtcgatttcg ttctgtgaaa
    16321 aggcggaggg ggtgggcaga taccagtttt caacattcgt ttgattcaaa gcattggaaa
    16381 ttaccaggat tttgcaacgc gacaagctgc ccctttcaaa cagtcccggg tatccttgca
    16441 ctttataacc atccctctcc agcagattcg caaacgcctg gtaccggcca tccctggtat
    16501 gaaaattatt atgtccttcg tcaatcaata tgaccgggcc ctggccagga ggataaaccg
    16561 gtacaggaat cgggggacgg tacgaagtat cggagatttg ttgggagaaa accggtgaga
    16621 tcgccagtaa taaaaagagg tggagccatg ctttcatgat gccgttggtc aaatttatta
    16681 cctataacga tcgtcaggtg aaggatgttg accggctaat cctttgacca ggttcggggg
    16741 atcggtacgc ccttcacccc ggagtatttg aaccccgggg tatcaaattt ttagaaaacg
    16801 ggtcaggcca acaattcgtc caggttaccc atggccatgg tgaacccttc cttaaagccc
    16861 atctcaatga tcttttccag atcggacact tcattgaagg atagagtgac atggaccatg
    16921 gtggattcac cgctggcttt gaattcattt agccagacat tgattgggaa ggaagtattc
    16981 acctccccgt tttcgtcaca aaatgcgtcc tgagcggtat aatagttcat cgggtcaatg
    17041 gcctgataat ccacacgcgc ccaatgccgg gtcccatccg gtcccaccat ggcgtataac
    17101 caatagcccc ccaccttaaa gttcatggac ttggtttcag cccgccaggg tttgggagcc
    17161 caccattggt cgagtatttc aggatccgtc caggcggccc atactttggc aaggggagct
    17221 gcaaattccc tttctacctt gatttttttg ttttcccggt ccaccgtgaa gttcattaga
    17281 agcgtatttt tcataatcaa ttgttttaat ttttaatccc ttaattattt taatttcttc
    17341 aatacttcgt caagctggtc aaacttggac tcccacaact gccggaaggg ttccagccag
    17401 tccgaaagct ccttcaactt cttaggctgg aaatggtaat agatttcccg gccggattgt
    17461 tcatggctca ccaattgaca ttcggtgagc accttgatat gcctggagac ggcctgcctg
    17521 ctggtatcaa aatgttcggc cagggcattt ggagtcatgg catgcatcgc cagcagagcc
    17581 aggatcgccc ggcgggtcgg gtcggctatg gcctggaaaa catcccttct ggtacttttg
    17641 gatttcatta tgcaataata tggttgcaaa tataatgcaa catttcggtt gcgcaatgat
    17701 ttgctaaaag tttttttttg gggggaagat ttaactatat cctgagggag actggcaggt
    17761 cgtcttgcgg ggttcgccac tttgccctgt ccaccccggc tgaccccatc aatgacttat
    17821 tccctgctga aacttcaaat gttcgccgat gaggagggtt gcggggaaaa gaacttgttc
    17881 ctccagattt gcatgtctca gcagttcctt ttcaaattcc cagacttccg agtgtttgtc
    17941 ctttgccgcc gcctgcttca gctcattcat ataggcgttg atcaactgat gttccacatt
    18001 caaatgaacc atctgcgact ttaatttttc cgttaataac aagatttctt ttatttgttc
    18061 gggccattgg gcgtttgcca aggaaggcaa gaggcccaaa ggaggcagtg cgtatccctc
    18121 ttcttcatta aaatgatgtt gcatcagttc ctccattttt ttggcgatca ccccggtact
    18181 gtcgggaaac atggtgattt gatgaatccg cccaagcagg tattcgtgct ctttcaggat
    18241 tgaagcaggg atttccggtt ttccgggata aaagggtaaa ggacgttctg gttcaggcct
    18301 gcaatcagaa aataggatta tataaaggag aataaaagcg gtaggtttta ttggatggcc
    18361 ggatatgcgg tagcgggtga tcatttttat cctgaccggc ctccttactt taaaaattcc
    18421 gaattaaagg aaaaaggcaa taagagctta atgctttcaa agacctttat ttttccctct
    18481 tcggttccca caatgatttg gatgggagcc ttttgtttgt cctcaaattc actgatcacc
    18541 tgacggcagg ctccgcaggg aaagccgggc tcggccagca tgatttttgg attcctgcaa
    18601 agcacggcga tggccaggat gggactcttt ggatcggaca tatacgcatg ggcgagggcg
    18661 acccgttcgg cacaaaggca aagcggatac gatgcatttt cgatattgct tcccggatag
    18721 acccgtcctt ttttgtttag caccgcggcg cctacataaa aaccggagta gggagcatat
    18781 gcataccgga gggcctgcct cgccctgcga agtaaaaatg ccggacccgg cctgaccgtt
    18841 ttttttgact tctgttttgc gatctttgcc ttattcattg ttttttatcc ttgatcaata
    18901 agatagaaat agcccgggag cagcggatcg catttcaggc agccctcgtg gcccgtccag
    18961 ccggattatt ggtcttttcc atcctgatcg cccaccgttg gagtttttct gaaattgctt
    19021 cgtttgaatc cctttccgta tttcaatacc ttgttttttt cgcctggctg ccctcctggg
    19081 gagcgattat gctcaggttg gcgggcaggg aagaatacaa acttcaaaaa ccattcctgt
    19141 tttcaaacct ggtcctggga ggactgattt tctgcctggt tttcggcgtg gccggattat
    19201 ttcggcaaaa cctgatgccg ggcctgcctg gccattccgc ttggtggttc aagatattca
    19261 tcctgatggc gttgtattac ctccagcagg ccggcattta cctggcttat ctcagaggcc
    19321 ggtccgtgcg gcaatggatc ctggctgccc tggtcttcag ttcctggatc tattgcggga
    19381 tatttaccta tgacctcagc agtgcggttt tcgcccagat ttttttcagc ctgggcatct
    19441 ggcttttgca ggtgcacgat tttgtttttg acaagctgag gtggtatgcc cccttctgga
    19501 aggaggtctt gtggtattcc ctgtacacgc tggccggggg ggcggggatc ttgctggctg
    19561 cgtattatgt gcaggacctt tatggacccg ggagggaatt caattggttc cgctacggaa
    19621 cgagggaaat accggtcttg cctgccctgc tggccgcttt cggttattcc tacctcaaaa
    19681 caggctgggg cgggcctggc attgaattcc tgtccggcat ctccaggcag atcaaatggc
    19741 tgatcctacc tttagccctc tttatcgttt ttgcgcggcc tgtgtttgcc tttgttttcg
    19801 gaccgaggtt tggagtggcc ggggacctca tgagcatcta tctgctgatt tatattccca
    19861 ggatgattta ttcccaggtg gtcctgcaag ccaaggatga agcgccctgg ttgttctggc
    19921 tgggcctggc ggagctcaca ctgatgatcc tggcagcctt gctcctggtc cccttactgg
    19981 gaattgaatc cctggtctgg atcctggttg ggggtaccgt ctttgagaaa atcatccaaa
    20041 tggcggtgct taaataccgg tttgccgtgt cgccttccga ataccttcct ttacccgggt
    20101 tctatatctg ggccgcaacg cttacagccg cttattttgt cagtcattgg atatgatatg
    20161 aaggtgcctg aatacattct gacagagtcc atggccctcc tggtcccggt gtttgggaat
    20221 cgggaaacca aggcccttct ccgggcactc caggaggacc tgttcgggtc cggcttcccg
    20281 gagtggacag ccgcgacggc gacaaggtgg aaggcggcca ttcaacggat ccttggcgga
    20341 gagcccctgg cttatattac cggggtcagc cactttctga atctcaaatt aacagtcaat
    20401 ccccatgtcc tgatcccccg gcccgaaacg gaggaacttg cggtcatggc aatccattac
    20461 ataaagagta tccatcatgc gcaggttttg gatgtcggca ccggcagcgg ttgcctggcc
    20521 ctggccatca aatcgtcctg tcccaatgca aaggtctatg ccctggatat tgattcaaag
    20581 gcgctggaaa cggccaaggc caatgcggac gcttaccatt ttcccattga tttcaggccg
    20641 tttgattttt taaatgaaaa cggttggacc agcctgccgg accatcttga tctcatcatc
    20701 agcaatccgc cctatatcgg tgaaacggag aaaacactca tgtccgcttc cacgcttcaa
    20761 tatgaaccgc cgcatgcgct ttttgcaggt acagaccccc tggttttcta caaacaaatc
    20821 gcccgttttg gaaaagatca tcttaatcca ggcggccgga tcatggttga gatcaatgag
    20881 ttcagggcaa aggagacgga agaggtgttc aggacggcgg gttacccagg gatcgaattg
    20941 tataaggatc tgagtgggaa ggagaggatt ttggtagtta gcaagttggc aagttagtaa
    21001 gttggcaagt tagtaagtta gtaagttagt aagtgggcaa gttatttcag gctgtcccac
    21061 aagggcttta ccggcctggc ctgatccagg ttgatgctgg tatccacata aggttttatg
    21121 ggtttcgggg ggacgggaat gctatcaagc aagaatcgct gggtgaagga gaggaaaaag
    21181 gaatcgacgg cgatttcggc tttctggatg gcttttgccc tgcagaggga atcctgttca
    21241 tcgcggtaga gttgcatacg ttgttcggcc aggagaatga cctctttagg cagctcttca
    21301 ctgccgcagg ccatgagaag gatcgagatg acaaccacct taacggaccg ggatttcatc
    21361 gtaacggctt gagtcaatca attttcggat ttcagtgagt ctcttagagt agatgctgtc
    21421 atatgcgatc ctgaacaagg agtctgtttt tttcaggcag aggctgtcca tggttggttg
    21481 ttgttctttg atcattcgct ggaccatggt atcgctcatc tccacccata attttacggt
    21541 ccggggattg gcttgtttgc aggaactgag ggtgaggagg aggataaggc tgaggattag
    21601 gttgaggttg aggttaaggt taaggttaag gttgaggttg aggttgaggt tgaggatcct
    21661 ggataaattg ctcaggctcg cacctcgcac cccgtacctc gaacctctaa cctccttcat
    21721 acctgtttct tcctaagttc aaaactgctt cccaggtaga cctttcgcac caatggatcg
    21781 gcagcaagga cttcggccgt tccttcccgc aggatcgccc cttcaaagat caggtaggcg
    21841 cggtctgtga tggaaagcgt ttcctgcaca ttgtgatcgg tgatcaggat gcctatgttt
    21901 ttcagtttca gtgcggcgac aatggtctgg atatcttcca cagcaatggg atcgatgccg
    21961 gcaaagggct catccagcag gatgaacttg gggttggtgg ccagggctcg ggcgatttcc
    22021 gtacgccttc gttccccacc ggagagggag tctcccttgt ttttgcggac tttgtgaagg
    22081 ccgaattcat caatcaggga ttccagtttt atctcttgtt cctttcggga cagatgggtc
    22141 atttcgagga cggcggaaat attgtcttcc acgctcagtt tccggaacac ggagggttcc
    22201 tggggaaggt acccgatgcc ccttcgcgcc ctcaggtaca tggggaagtc ggtaatatcc
    22261 tgggaatcca ggaagacttt gccgctgttg ggtttgataa aaccgaccac catataaaag
    22321 gtggtggttt ttccggcccc gtttggcccg agaagcccaa cgatctcacc ctgtttgacg
    22381 ttgacgctta cgtctttgac cacttcccgc tggccgtacc gcttaaacag gtgttcactc
    22441 ctcaatatga tcttgtcatc catgttttat tggtttgtcc aactgatttg ttcgcggatt
    22501 tcccaattgg cccgtacgct gacgtttttc cggatcagga ctttttcggg caagaccttc
    22561 tctttatagt ccccgggatc ccaacgcccg tttttattcc ggtcttccgt gatgcgaacg
    22621 ctgaacgtgc ccgggtttag aaaggaatag gtgagggtat aggcggtttg agcggtatgg
    22681 gaggaactgc ggagggcttt gtcgtcctgg atcaggtcga tgacataggt ctgattggta
    22741 tccaattcag tacattcaat ggtaagcaat ccggtttgtc cggcccccaa gcctctcagt
    22801 ttaaaaatca gggtgtcggt cgtgtttcct ttataatcag gaatggcccc gggcaggagg
    22861 gtgagggtat aggtctgctg ttccatcccg ttgaaggtaa acacaagttc attgaccaac
    22921 ttgcctgatt gcagttggaa gggttcggaa aaccggctgg tatcattgac caggaggacc
    22981 cggctggtat ccggcagacc cagggggtgg ttgaagttta gcgtaaggcc caccccggga
    23041 gccacggatt ttatgtcctg acgggtggtc atgcatttaa aagtatcagc gagcatttgc
    23101 cgtatccggt ttactttaac ggtatcgacc ggttctcctt ttaaatggac ctccagggac
    23161 cagtcccggc tcgtatcagg caggtaatac accaggaggg tatccctgta attcatgggg
    23221 aaaaggcgga cctgagcttt gtccgctttc agttccatct cctcataagg aaaattggcc
    23281 atgattttta ccactcctcc ggtttccgca tcctgctcgg tgatcctggg gcgcacgaag
    23341 gaagaataat atcgaaggag tagggtagtg ctgtcttctc cggcctgcac ggtgcggtcc
    23401 agaaaagcga tgatttcctt gtcctggtca tagaggtaat tggaattggc atcttccagg
    23461 gcaaagattt tgtaaacggt gtccttgatg ttgcggatga taaaatggcc gttcttatcg
    23521 gtccggctga aataggtggg cctttccttg aagaccacgg tatcgtgctg gttttggtac
    23581 aacatcacga tcatgttttc tttggtttcg ccggtgtagg catcagccag cttgcccccg
    23641 atataagccg tatcgatgat ttcccctgtg ctgaatacat atcgaaggtc tttgatcgga
    23701 ttgttttccg tgatgtcctt gatggcattg ccaaagttaa tggtatacgt ggtattggct
    23761 ttaagttgtt ccctgggatc gaagcgcacc aggacgccct tgcccttgag ttcaatcttt
    23821 ggggcatact ggagcggggg ggaaaccact acttgtttga acgcgtcttc cagtttcacc
    23881 cattcgttga accggaggac gatatcggtt ttggaagccg ccgggtcaaa attgcgctga
    23941 aggtttggtg tggattcggt ggaatcgatt tccggagggc tgagatcccg cggaccgcct
    24001 tcggggtttc cgatattggc acaccggagc caaaaagcag agattactat gaggccgatg
    24061 tatatgttgg gattgggtcg caattttcag gtccaaaggt atgaaagtcc gtgggtccgg
    24121 gggagggaag ggctggttcg cgcagagggt tggttcacac ggagggttgg ttcacacgga
    24181 gggcgcggag ggcacggagg tttggttggc atgcagttgg ttcgaacgga gggttggttc
    24241 tcacggaggg cacggggtac acggaggttg gagatgactg ttcatttttg ggcattcgtg
    24301 ttactttttt ttggtcaagt tttgtaagac aaggtaactc acgtataaat tggatatttt
    24361 aaaatatccc ttcgtgaact ttgagccttt gtgggcccaa aaccttgtgc tttgtggtaa
    24421 ccctccgtca gtgggccatt ttgaacctcg gatgaaacct gtataaggtc tccctcagat
    24481 aatccgtatc caggtgagta tagatctccg tggtggtaat ggattcatgg cccagcatgt
    24541 cctgcacggc tttgaggtct gcaccccttt ctacaagatg agtggcgaaa gaatgacgga
    24601 aggtatgggg gcttactttt ttgtggattc cggctttggc ggccaggtct ttgatcagtt
    24661 tgaataccat gatgcggctg catttttttc ctctgaggtt cagaaagagg atgtcttcat
    24721 gccctggggc gatatggggc atgcgtcgcc tgactccttc caggtagagg tggatatgtt
    24781 tgagagcttc ggaaccgatg ggtacaatcc tttccttatt gtttttcccg atcaccttta
    24841 caaatcccag gtcgggatag agcatggaca agcggaggtt gatcagttcg ctcaccctca
    24901 ggccgcaggc atagagcgtt tccagcatgg cccggttacg gactccgctg tccgtgctga
    24961 ggtcaaccag ggccaggagg gcattgatct cgtccaggct caggacatcg gggtattttt
    25021 tcatcaaccg ggggccttcg aggagttcgg tagggtcatc catgaccagg tcctctacca
    25081 ggagatattt atagaaggct ctcactcctg aacggactct cgcctgtgac ctctcgtcca
    25141 ggccaccctg gtccagaaat tggacgaagg cttcaaggtg gcttaagctg attttttcag
    25201 ggcccgtttt cgggtaattt tccagggcga atcccgccag tttttctgca tcccggatat
    25261 aggcttccac ggaattgacg gaaaggcccc gctccatttt gagataggct ttaaatcctt
    25321 tgacgcttga atgccagtcc atgagacaaa tatagattat attttaatct aatatgagtt
    25381 taaggaggaa tggagacagg aggaaaggag accggagcga agagaccaac caccatacac
    25441 caagcaccaa ccaccatatc gttccaaatc catatatttg cgcctcgatt ttgaaaaaag
    25501 ttgtggtcac tccccggaaa agccgggtta tgatcctata taatgatgtg gtagctcccc
    25561 gatgaagatt ggggcaaatt gccggttgtg agaatgaaat aaattgaaag gtaatggttt
    25621 ggatttcccg ccttatcggg gcaggcagct ttcagcttcg ctaccaagcc attcctgaag
    25681 tcatggcgtg gtagctcccc gatgaagatt ggggtaaatt gccggttgtg agaatgaaat
    25741 aaattgaaag gcaatggttt ggatttcccg acttatcggg gcaggcagct ttcagcttcg
    25801 ctaccaagcc attcctgaag tcatggcgtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnnnnnnnnn tcggggcagg cagctttcag cttcgctacc aagccattcc tgaagtcatg
    25981 gcgtggtagc tcagctggtt agagcgtgcg attcataatc gcaaggtcgg gagttcaagc
    26041 ctccctcacg ctacgaagca agtggtcagt agtaagggat agaaatcatc ccgtctttag
    26101 caaggagttc atcctggagg aaatattttt ccttcacgcg gccaggtcac tctgcaggta
    26161 aacgcttttt gaattgcgtt cgatcggatg tatcttgttc tcaatgttta agcatcagat
    26221 caatggaagt tttgatccgt gggtcagggt tgggtttatc gttctgttga tcgccattcc
    26281 gctttctttt tcaataaata ttggatcgag tcagctcaat atcctgtcgg aaccgctgat
    26341 cgccttattt gctgtggccc taatcggttt gataaagggg aagacctttt tcaggggtaa
    26401 ttttatccgt catccgctga gtattctcgg cttgatttat cacgggtgga tgatcaccat
    26461 gatccccttc tcttcgaatc catgggtgtc cttaaagtac accttggtca atgcggccca
    26521 ttattgggtt ttctattttg gattttattt ttttgtcaaa cacacgggta aaaatatcct
    26581 gaattggatg gggtcctacc tgctgtcatt tatgattgta atcctgtttt cgtggtacaa
    26641 ctattcatgg tatggattca gaatggacgc tgctcccgtt atggccctgc cattctataa
    26701 tgatcatgcc ctgtatgggg caagcatcgc cctggtcctt ccttttgcat tgctgattcc
    26761 tcaaaattat ttaaaaaagc cagggtccct ggttctgacg ggtattttga tgattgccct
    26821 ttttttatca tccagcaggg ctgcgtggct aagcttcggt gccggcggta tatttatttc
    26881 agggctaaag tttttgcggt taaaatttat aacactgacc ggtttgatcg ccatcctcag
    26941 ccttttgacc atgcttaatt atacaagcct gatccggttg attggcagca atcgggaaga
    27001 atccaggaaa ggatcctgga tggaacattt taaatccatc gggaatatca ccaccgatgt
    27061 ctccaacctg gaacggctca acaggtaccg gtgcgccctg aggatgtggg cagaccggcc
    27121 tttcactggt tttgggccgg gtacctatca atatgcttac ctcccttatc aaaaacctga
    27181 tgaaatgacc cgcctgagcg tgaccacgcc gcgcgccccg gacggcgggc cgcatccaac
    27241 cgggcggggg ggtggggccc attcggaata cttgcaggcc ttatccgaat tggggctgcc
    27301 cggttttttt tgttggatag gccttctggt gaatggttta tataccggta tgaaaaggta
    27361 ttaccaggaa aaaaatagag agcatcagtc cttgttgctg gccattcttt ttggcctggt
    27421 gacttatttt acccatgctt ttttcaacaa tttcctgcac agcgaggaaa ttgctgtcct
    27481 tttttggccg gcattggctg ctttagcctt cctggacacc gaaagggaac caggggctca
    27541 gatcaaggac ccgtaaactt cagcaatacc cctgaattga tttagggttt atccccgcat
    27601 aaatggtatg gttcctttta gtttaccaat accatctttc tgctgtctgt aaaaaatccg
    27661 gattcaaacc ggtagtacaa tacccccgtg gccccgatct ccctccggta aagccagacc
    27721 tcgtgatagc ccctggtcca gtctctttct atggagcgga tggtccttcc ggtcacatcg
    27781 tgtatgctca gcgtaccccg gccctctttc gccagcctca ggccgatcac cgtgcgctgg
    27841 tcaaagggat tgggataatt ctggtataac tcgcttcttg tgctgacctc attgccttca
    27901 ggacttacaa accggatgcc gaggttgccg ggctcaccct ttccttcata agactctgca
    27961 actggctggc gacttaaatc aatcgggtaa atttgatctc attcatagct tctggctggg
    28021 accttgttgg ctcctgggcc gccatcttca gaaaagatgg agagtgcctc attttaccac
    28081 tctgatggga caagatgtcc tgaagtccaa ccggtatctt cattttcttt caaaaacaga
    28141 agctgcaaat ttgattacgg tttctgaatt tcagaatgag tgcctggtgc aatccgtggg
    28201 ttttccggcc ggcaagtgta tcccatgggg attagccagg gaggatatgc cagctgcttt
    28261 tccggaccaa cgtcccgtgg atgtcctggg ttgcggttcc ctgatcccgg ttaagaactg
    28321 gaacctttgg ctcgaagtgt tggccatgct ggttcgatgg aatccggcct tgaaggccga
    28381 aatcattggg gacggcccgg aacgaaaaag gctggaagaa cagatatgga aaaacaaact
    28441 ggaacggaat attagcctga cgggtaccct tccccggccc ttggtgctta agaaaatgag
    28501 tatttccaga gtctttttac atacctcagg ctacgaatct tttggatatg ttttggctga
    28561 ggctgcagcc aatggatgcc atgtggtcag cactccggtc ggcatcgccc gggaccttgc
    28621 atcgtgcgca aatacagccg aagcgctttt taatcatgtg gcccgattgc ttgttttgcc
    28681 tcctcctgcc gggaaagtgc cggtaaagac aatggaggag gtcgccgctg aatacctgga
    28741 tctttacaaa aactgcaaaa aagccgactg aatcgatcag gatacaaccc agtctgaggg
    28801 tctgtgcttc aaatttttgt aaaaatagtt tttaatagga ccctcgctga gcaagcggat
    28861 cgcgtaataa tggacctgga acctaagctt gttcttgagt tcctccagat aaaaaagcct
    28921 caggaaaaat ttcagtttag cccaataaac ccggataagt aaaaacaagg gtatcctaac
    28981 ctggatattt gggaaggtat agggcagcgc atggatttca ggggacaaat atctgtggag
    29041 cattcctatt tgttccgcgc tgagtatatc caatgagcgg tcgattcgtt gatcggtgag
    29101 aggttcatcc agcctgccaa gcaagaattt catagcctct tttccgcccg tatattcttt
    29161 acgaaattga aagtctttca tgcccggctc gaattcaagc cccaggaagg tgcaaatttc
    29221 agtgagggtt tgatctggct tcctgaccag gtcttcatag gtgatagtca gggaatccag
    29281 attcctgagc cgctgcctta taaaaagttc atgcaatcct cttttggtgt tttcggcaaa
    29341 gtgtaatagc agggcgtccg tgatcctgat ttttttatgg aaagcccaag cctcacacaa
    29401 ggataaaacg agatcaaaag ggtttctcag gataaggagg tatttactgc cggggaaagc
    29461 cctgtttaag gccggaataa aaaatgtgtt ttcaggtgtt ttttccccgt ataagtttgc
    29521 ttttttttct ccaacatatt cccggataag gggtgagaac cattccccgg tggatatggg
    29581 ataagtaaat gacctcaaag cctggtgggc caggcagggc aagatcgggt ccttaaaggt
    29641 ccgggccctt tggtacaaag cattgaccag tagaaggctt ttccattgat cccggagtgt
    29701 tgattccgcg ttcaagcgcc agagggtctt gcccgtcgac atttcagggc aaaccgcaat
    29761 ttcagagtgt gcgtccagca tctgttcaag cagggtagtg cccgacctgg gaagtccaag
    29821 tataaaaaaa tcagggcctt tcttatcctg catatcctcc gcggttacca tccggatttt
    29881 tcagcttgcc gtatcatgcc ctattttttc taaaataaaa tgcagcagtt tttctgctga
    29941 ttcttccggg gattcccggt cagtgtggat caccaggtca gcatgcaaag ggggttcata
    30001 cgggtcattg atgccggtca ggttttggat ccgctcaggt tggcctggtg tcaggaatgc
    30061 ccggcggtac aatcccttgg gatctctccg gaccaggagt tccaccttgc agtcgagcca
    30121 gatcgtgaaa attttgttgg attggctgtg caaggccctg cggtcttctt cgtatggatt
    30181 gatggtggca atcatgacca atatcccatg tttcaggaga agcaggccaa gaaggcccag
    30241 cctgcggtta ttttctttac ggtcctcgcg ggtaaagccc aggtcgcggc acaaagtccc
    30301 ccggtattca tcgccatcca atacctcaca ggggaaaccc aggtcaatga gcaacagctt
    30361 cactcggttc gccagggtgg ttttcccgga accggataaa ccggtgaact gaaggaggag
    30421 cataagtaag ggcttaaatg tacatcagat cccatgaaca tcccctttcg ttaaaagaaa
    30481 acaggcccaa cttttttatc ttgtgcctgg tcccatgcaa accccgtccg aagcgtccct
    30541 taaaaaagcc ctcagtcccc ttatgctctg gggcctgggt gtcggttacg taatctccgg
    30601 catgtatttt gggtggaacc ttggactgaa ggaggggggc accctggggc tggccattgc
    30661 aaccttcttt atcatcatcc tgtacaccac cttcagtttt tcttatgctg aactggcctg
    30721 ttccattccg aaggcaggcg gagtatttga ttatgccaac agggctctgg gtaaaaactg
    30781 gggatttctg gcggggatgg ctcagatcat cgaatttgta tttgctcctc cggccatcgc
    30841 ttatgggatc ggggcttatt tcaacctgtt ctttcccggt ataccggtgc tggccatcgc
    30901 catcacagcc tatgtcttgt ttacggggct caatatttac ggggtaaagg cggcggcttc
    30961 ttttgaattg atcatcaccc tgatcgcggt ggtggagctg ctgatattcg ttggagtcgc
    31021 cctgccgcat tttgaagcag ccaatctcaa ccacaatgct ttcccgaatg gctggggagg
    31081 ggtattcgcg gccctgcctt tcgccatctg gtttttcctc ggcatcgaag gcctggccaa
    31141 cgtagccgaa gagaccaaaa acccacagca agacattctg aaaggctttg gctccgcgat
    31201 cctgaccctg gtgatccttt gcatcctgat tttcctctcg gctaccgggg ttgggggatg
    31261 ggaacaaata gtctataaag acggagtcgg gggagaaact tcggattcgc ccctcccttt
    31321 ggccatggct caggtggtag gcagtgacag cgtgcttttt cacatgttgc tgaccattgg
    31381 attgtttggg ctggtggcct cctttcacgg cctcatgctg gccgcaggca ggtcgacttt
    31441 cgagtttggc agggtgggat ttgccccggg cttcctgggc agggtccatc cgcgcttccg
    31501 caccccggcc aatgccttgc tggtcaatat ggtcctgggc atccttgccc tgctgaccaa
    31561 taagaccgga gagatcatca ccgtttccgt actgggcgcc ctcacccttt atgtcctggc
    31621 catgatctct ctccttgttt tacgcaggaa agaaccggat gaaccccggc ctttcaaggt
    31681 tcccggctat ccctggaccc ccggcatcgc cctggtgatc tcgctccttg ccctggtcct
    31741 ggtcaattat tacaatttaa aactgaccct ttattttttg gtcttgatgc ttttacccta
    31801 tttctttttt aaattatttt acaaaaaaac agagacggta tgagtgaaca ggaagttttt
    31861 aattatgtaa aaaatcatcc cgatcagaaa atcaaactcg ccattgcgga caccgacggc
    31921 attctgcgtg gaaagtatat cagtaccgaa aaatttttag ctgcagccgc ggggcacctc
    31981 ggcatgtgca atgtggtttt cggctgggat gccggcgatg cggcctacga caatgcccgt
    32041 tacaccggct ggcattcggg ttatcccgat gtgccggcgc gcatcgatct ctccactttt
    32101 cgcaaaattc cctgggaaaa cgggatcccc ttttttctct gcgaattggt ggacaaaaag
    32161 gaaagtccgg cctacgtttg cccgcgaaac ctgcttaaaa gagtgcttgc cgaggcagag
    32221 gaaatcggat acaaacccat tttcagccag gagtttgagt ggtacaattt tgaggagacc
    32281 ccgcattcgg cttcggataa aaaattcagc ggtctgaagc cgctgacccc gggcatgttt
    32341 ggatactcca ttttgaggac tagccttcga aatgattttt tctccgccct ttttgatgaa
    32401 ttaaaaaaat ttgatctgcc cctggaaggc ctgcatacgg aaacgggtcc cggggtttat
    32461 gaagccgcga ttgcctattc cgggatcctg gaagccgcgg accgcgccgt ccttttcaaa
    32521 accgcggtga aggagctcgc ttataaaatg gggatcatgg ccaccttcat cgcaaaatgg
    32581 gatgaaaacc tgcccggttg cagcggtcat gtgcatcaaa gcctttggga tcaggaagga
    32641 aaaaacaacc tgttccacag cgaacgggac aaaaccggaa tgagcgaact ctttaaacaa
    32701 tacatggccg gtcaactgtt ttgccttccg catatcctcc cgatgtacgc ccccacggtc
    32761 aactcctata aaagactggt ggaaggagcc tgggcgccta ccaccctcac ctgggcgatt
    32821 gataaccgga ctaccgccct gagggccctg cccggcaata aaaaatcctg ccgcctcgaa
    32881 acccgggtgg tcggatccga tgtcaatccc ttcctcgcca tggccgcctg cctggcgagt
    32941 ggcctttacg gtattaaaaa caaactgaaa ctgcaagcgc ctaccaaagg caatggctac
    33001 cgggatacgc ttcacggcgt tctgccttcc aatctctgga tcgcaagcca gaccatgaag
    33061 gaatcgcccc tggccaaaga actgttcggg ccggaattcg tggatcattt caccgcaacc
    33121 cgggaatggg agtggaggca attcagccgg gtggtgacgg attgggagaa gaggaggtat
    33181 tttgagatta tttagcaagg aggaggagat cgcacggagt gggggggcgc acggaggagg
    33241 agatcgcacg gagcgcacgg ggagcacgga ggagaagtat atgaatttag tgaattcatt
    33301 tccttttcgg tgcacctttg cgaaccttgg cgatctctgc ggtgaagctg gtgaccgtac
    33361 cgccagggat gcgacattaa acccagagga acaatccgtt tccatgtact cctttgctcc
    33421 gagtgaaacc actcccttga gcgcatttta caatctctca caattattct tatattttta
    33481 taataagccc caggaattaa ccgcaatggt cgcagagcgc gcgaagtttg cgcaaagtga
    33541 tttagtgaat tcatttcctt ttacggtgca cctttgcgaa ccatggcgat ctctgccgtg
    33601 aagcaggtga ccttactgcc agggatgagt cattcaaccc agaggaacaa tccatctccg
    33661 aggactcctt tgctccgagt gaaacacttc caagagcata ttttacaatc tctcacaatt
    33721 attagacctt tgaacctttc aattcagaac tcttcgccta attaaaaccc aatggacttt
    33781 aaccagattc ggcaatttaa tttccccacc ctcatccggt ttggcgcggg ggcggctcag
    33841 gaactggctc cctatctcaa agacaaccaa ttgtccaggc cgctggtggt gacagatccc
    33901 caggtggcgc aacttccttt ttttaccaag gtcatccggg aactggagaa taacggcttg
    33961 tccgtagaaa ttttctcttc gattcataaa aacccggtta aatctgatgt ataccttgga
    34021 accgatcttt gggattccag tgaccgggac tgcgtggtcg ggattggagg aggggcggct
    34081 ctggacgtgg caagggccat agtgctgagg gtccatcacc gggaagatct ttttaaatat
    34141 gacgacctgg tcggcgggga cgtctatgtc accggggagg tcccccattt tgtcaccctg
    34201 cccaccactg cgggcacggg cagtgaagtc gggcgttcgg ccatcatttc cgatgatctc
    34261 agccaccaga aaaaaatcct tttttcccct aaactcatgg ccaggagggt atttgccgat
    34321 ccgctgctga ccatggatct cccctctgcc ataaccgccg caaccggcat ggacgccctc
    34381 actcataata tggaagcctt cctggcaaaa aattaccacc cgatttgtga aggcattgct
    34441 ctggaaggca tgcggctgat ttccctttcg ctggagaagg ctgtgaacgg cccgaatctt
    34501 gaatcccgcg cacaaatgct gctggcatcc atgatgggcg ccatcgcctt tcaaaaaggt
    34561 ttgggcgtgg tgcattccct ggctcatccc ctgtcttctc ttttggatac ccatcatggg
    34621 ctggccaatg cggtgaatat tccctacggc atgaaattta atatccaggg ttcggaggat
    34681 aaattttcaa ggatggccca ggcgctggga ttaaagacct ggtctggtaa ggccgtagtg
    34741 gacttcctga ttcagctaaa tgaaaagatc ggcatccctg cccgcttgaa ggatatcggc
    34801 gtacaacagc aacatttgga ccccctggcc gacctggcca tcgccgattt tgcccacccc
    34861 aacaatccga agccggtcag ccgccaggat ttccttgcct tgtatgagga ggccctttga
    34921 ggaaattttt ttaaactccg gaatgttgtc ataaatcatc cctaagaatc tgaaatgatc
    34981 accataggaa ttaccgagac ggatacaaat tttaaacact acctgccctg gatccagggc
    35041 ggggacccgg atattgcagc cctcatcctt tcctataaat ccccaaacct ggatgacctg
    35101 ggcaaatgcc acgggatcgt gctcagcggt ggcgtggact ggcatccgaa attttataac
    35161 aaccccagga cagactatcc ccgtgcagat cattttatcg aagaaaggga tgagtttgaa
    35221 cttgccgtct ttaaatatgc ccgtcaatac cggttgccgg tgctcgccat ttgccgcgga
    35281 atgcaattgg tcaatatcgc cctgggcgga gacctgatcc aggacctgga agaatccggg
    35341 aaaaaaaatc accggaggat gaacggggag gacggacttc atcaggtaaa ggtcaggccc
    35401 ggcagtttat tgcatgatat tgtacaccgg gaagaaggga tggtgaattc ggcccaccac
    35461 caggccctgg gcaggctggc agaggaactt tgctccagtg cttcctccga ggacgacctg
    35521 gctgaggccg tggaatataa atctaaaaaa gaccaggggt ttttattagg ggtccaatgg
    35581 catcctgaac gcctggatat ccgcaaaggg agccccgctt tttcgaaaaa tatccgggat
    35641 gccttcgtcc gggccgtccg ggaaaaacag gaatccagtc accacccaaa aaattcactt
    35701 caatgaatat catcaacccg gctacggaag aaatcatcgc aaccgtgaag gaggatgact
    35761 ctttcagcct ggaaaaaaag ttggctttgc tcaaaaaagg gcaaaaaacc tggcaagccg
    35821 ttcctttaga ggaaaggatt aaacgcatcg atcgctttgc aggccttctt aaagaaaata
    35881 tcgaatccct ggcctccacc ctgacctctg aaatgggcaa gcccctccag caaagccgga
    35941 atgaaatcaa cggggcctcc aaccggatcg gttggctcac cgggcatgcc ggtcaatttt
    36001 tatccgaaga actgatgaac cggtccgggg atatgacgga aaagatcgtt tatgagcccc
    36061 tgggtgtggt ctgcaatatc tcggcctgga attatcccta caatgtgggg gtcaacgttt
    36121 ttgtgcccgc cctgctcgcg gggaatgccg tcctgtacaa accctccgaa tacgccaccc
    36181 tgaccggcct ggggattgcc cgttatttgc aggaggccgg gatcccggag gaggtgttcc
    36241 aattggccct tgggagtggc gaggtgggca agctgcttct ggaccttcct tttgacgggt
    36301 attttttcac cggctcttat aaaaccggta aatacattta tgaaaaggtg gctccgaaga
    36361 tggtgccttg ccagcttgaa cttgggggta aagacccggt gtatgcggcc gacgatatcg
    36421 aggatataaa aaattcggcg gcaggcattt cggatggcgc cttttataac aatggccaga
    36481 gctgctgtgc ggtggaaagg atttatgtcc atgaaaaaat atatgaggcc ttcatacagt
    36541 ccttcctgga tgaagtgatg tcctggaaga tcggcgctcc cgatgaagcc ggtacctata
    36601 tcagcgtact cagccgcaag gctcagctgg aggtattgga ccggcaggtc gccgatgccg
    36661 tgagcaaggg agccaaagtc cttgccgggg gcaaacgcct ggaccggaaa ggttattatt
    36721 acgaacccac cgtgctgatt gaggttgacc attcgatgga gctgatgaag gaagagagct
    36781 ttggacctgt gatcggcatc atgaaagtca aaaacgacga ggaagccacc cacctgatgc
    36841 aggatacgga atacgggctt acagcctcgg tgtattcctc ttcctgggac cgtgcacaaa
    36901 aaatattgtc ccgcctggat accggtaccg gttattggaa ttgctgcgac cgggtgagtg
    36961 cagcggtccc ctggagcggg agaaaacatt cagggatcgg gagcacgctg tcccacgcag
    37021 ggctcagggc atttacgaaa ccgaaagcgt atcagttgcg agctacgggc agcgagtagc
    37081 gagaagtgag tttgtcaatg cctgacagag gttgaccgtt tttaaggttg aaaaacactt
    37141 aaaaac
//
LOCUS       DAOQXT010000040        36593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1332_length_36593_cov_5.347665, whole genome shotgun sequence.
ACCESSION   DAOQXT010000040 DAOQXT010000000
VERSION     DAOQXT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 36593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36593
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1332_length_36593_cov_5.347665"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PKM27_08935"
     CDS             complement(<1..1104)
                     /locus_tag="PKM27_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNR07426.1"
                     /translation="MATEALDTLSLATKYCGSCHRFPEPGLLDKKTWEHSLLPNMGCR
                     LGIRTEGYSPYKGKDMLEQNLLEQANIYPALPLIPDSTWFKIRDYYISNAPDTLIVPR
                     DSSVDLEQFVVYPKGALVRVPRLSMLAVDTFSGLVFAGLESGQIRVLDSSWDLSDSFA
                     IGSTPIQLIPGEEGTRILSVGILYPSEQSFGSLLTRKGGSWQEEINGLHRPVWLSLMD
                     LDGDAESDYIIHEFGYETGRLSWYPSSRKDPKAVSLFSGSGSIKSEAVDLDGDSRKEL
                     VCLIAQGDEQVSAWLPVAGGAPRYQRLLSFPPVYGLCDLDLIDFNGDGRVDILISNGD
                     NADYSQVPKPYHGIHIFLNQGGFRFEESAFIPYP"
     gene            1308..1880
                     /locus_tag="PKM27_08940"
     CDS             1308..1880
                     /locus_tag="PKM27_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR07427.1"
                     /translation="MTAPSVALTFTSDPEILEDVLTLQRLNLRKFLSPEESAAQGFLY
                     VEHDPVTLKSICRSEPAVVALDGKRLAAYALCMTREHGHSVPELFDFFNLVDSLHVEG
                     RALADHGFLVCGQVCVAKEYRGQGLMTSLYHKMSEHRSKYYYCVTEISSRNTRSLHAH
                     SKVGFVPIREHRHADGESWQIVLWDWNKIN"
     gene            1912..2391
                     /gene="ribH"
                     /locus_tag="PKM27_08945"
     CDS             1912..2391
                     /gene="ribH"
                     /locus_tag="PKM27_08945"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNR07428.1"
                     /translation="MSKRTNLVESALPELPSMAGYRFCIISTAWNREITEKLEVAAIK
                     TFVRHQVKKQDLEVIRVPGSFELVFAAKRIIKKSRPDAVICLGCVVRGDTPHDVYISQ
                     AVSQGIMQLNILRNTPVIFGLLTTLDQQQALERAGGKEGNKGTEAAITAMHMALLND"
     gene            2391..3251
                     /locus_tag="PKM27_08950"
     CDS             2391..3251
                     /locus_tag="PKM27_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR07429.1"
                     /translation="MMVLKSVLAGTFKLDGGAMFGVVPKRIWSKLMQPDANNLCDWVM
                     RCLYVEDRHHRILIDTGMGNKQDTKFFSYYEPGGFRTLQAALNHHLIDPESITDVILT
                     HLHFDHVGGALFYDEAGNPAASLPSATHWTHKEQWDLAVKPNAREKASFLPENFMPLL
                     AQDRIRFLQDGDSPFPSIEFIRVDGHTRAMVLPLIHAGPDKKLLFTADLFPSVHHLAL
                     PYIMAYDMQPLVTLAEKELLLNRCIEEKIDLYFEHDIDHEVASLKRTETGKVVAAAFT
                     TLEEWGVKTG"
     gene            complement(3238..3783)
                     /locus_tag="PKM27_08955"
     CDS             complement(3238..3783)
                     /locus_tag="PKM27_08955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNR07430.1"
                     /translation="MILIVHGTNRRGSLSKPLTDFVTKYLNETSGEEVESLDLAEIPI
                     EAFDSESMYQADRHSSRLMDIQNRYVFPASKMVFISQEYNGSIPGILKLFIDACSVAD
                     SKKAFYHKKALLIGVASGRAGNLRGMEHLTGILMYLNMIVYPNRLPVSRIEHLIDEQS
                     ILADQDTRNAITRLLDQFIRF"
     gene            complement(3968..6151)
                     /locus_tag="PKM27_08960"
     CDS             complement(3968..6151)
                     /locus_tag="PKM27_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR07431.1"
                     /translation="MKKIIVWTLWFGCTGLYSQNEDTLMVKEISKIEIQGYRFEAGAV
                     RQLEAVHQTYITSGKKNEVLTVQDLPANLAEKTGRQIFAKKPGIFVYDMDGSGNQVNV
                     SNRGLDPHRSWEYNVRQNNIMTNSDIYGYPASHYSPPMESISRIEMISGTSSLQYGSQ
                     FGGMINYVTKQGDTTKALSYESLSSIGSYGLLSTYQSIGGKSGRFRYYAYYHRRNSKG
                     YRDDSRSDAQSQFFNLTYAASHDFNIKAELGRSQYLYQIPGPLSDAMFRDNPRQSTRR
                     RNYFSPDIYIPSLSLDWKISPTTRLNWVNSAVLGTRNSVQFIGLADTEDKIDPLTGQY
                     KPRQVDIDHFNSYSSELRLQQHYRLGKVGGILVGGLRLVNNDLHRRQQGKGTTGTDYN
                     LEITESSFGRDVHFKTDNTAFFAENLFQLSPRLDLSVGLRLESGTSKMRGIISYLPDE
                     KVPQDIDHRFPLVGISGQYRLNSNHLVYGGWSQAYRPVIFADIIPATVLDRSDPDLKD
                     AYGHNAELGIKGQISAKLKYDLSLFRIYYKNRIGTHVLTEGAGETYFLKTNIGNSRTH
                     GIEAYAEWNLIDQATHRLSVFTASSYFDAIYLNGKLREGNENKSLEGNRLETVPEWIS
                     RNGIQFNYKLFTAVFQHSYVDDIFSDAVNTVQPSANGAKGRVPSYTILDLNMSYRFNA
                     NYLLKLGINNLANRQYFTKRPAGYPGQGVWSSDGRSLVVSFGIRL"
     gene            complement(6408..7877)
                     /locus_tag="PKM27_08965"
     CDS             complement(6408..7877)
                     /locus_tag="PKM27_08965"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNR07432.1"
                     /translation="MNINLLRLCLPFTLLILVASCQKNTIEEPGEEVPETNLVADYDH
                     SVIWEWNELFARIDKDALGYRPCPGPRALAYMGLSAYEIAVPGMPTFNSLKTQWGTDL
                     KIPDFQVGQNIHWPTAINSSYAFLMKKFFFKTNFVQGFGHISNAEAQKMIATLETGLE
                     TKFKAEINDLVLFNDSKAWGEKVATAIWNWAITDAYGHESDLNPLSNDPTKGDAFYYN
                     WRAKSMVDGKVVPGRWQPTNDNPDGGMFPYGGRMRTFVTNDAQKLCPPPLAYSESKTS
                     PFYAQALEVYSLSNPDMRYEDRWVSEFWSDDIFGQTFSPPTRVIAILDQVLVIEKANL
                     EKAVESVAKLGLGLNDYGVACWHSKYVYNVERPETYIKRLIDPNWEPILVNTVSGVQG
                     ITPSFPAYPSGHSTFGGGAGVLLADLFGQNYEFTDLCHKDRTEFLGIPRTFNSFEDAG
                     YENALSRVTLGVHFRMDCVAGVALGQAISRRVLKLPWKK"
     gene            8057..8734
                     /locus_tag="PKM27_08970"
     CDS             8057..8734
                     /locus_tag="PKM27_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR07433.1"
                     /translation="MKILLIEDEPKTALSIQAFLNENGIETDLCLNAEEGLRKIDRSP
                     FDVIVTDVIMPDMNGIELSRKLRSKQIKTPILMISALDETDDKVKGLDSGADDYLAKP
                     FDLKELFARVSALHRRSNLSNLSAEKLNYEDLEINLSTLEVSRAGRKIILTPKEFSLL
                     EYLIRNQGRVIPKLEILEKVWNLGVEINTNVIEVYVNYLRKKIDRDFTDKLIHTHFGI
                     GYYIKKE"
     gene            8743..11514
                     /locus_tag="PKM27_08975"
     CDS             8743..11514
                     /locus_tag="PKM27_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNR07434.1"
                     /translation="MKIRTKLSYQFILITIGIYGLSMLFIYRQFKAHIEEEIFSLLES
                     KARMTAEMVLFHEDELQPLPDKKEPGEILIQDVGNTSIYNNDLNRIYSLISSAPNTPV
                     SALRQIRETGDYRFINPPYKAYGFLYKSRQNKDYIVVSEDKPDYSKLVKLRNILLIST
                     LFTVLIVTFGGWFFAGQSLRPVSRIIHEVKHILPTDLSKRLKPDRHNDELTNLIATFN
                     SLLERIDQAFRTEKSFISNVSHELRNPLAAIRTQIQYAINKKRTPEEYNQILSSLQED
                     IDEMTQTIEKLLQLARVNSNANHIPLTAVRLDELIYQAQESLYKSNPSYNIKIEMMHL
                     PVEEEGMFVQANEPLLKLAFMNLMENGCKFSNDSSTRTILDFSDPEEIRLEFQNSGTA
                     ISPDEITKIFQPFYRTTEHKHRKGSGIGLSLVKSILDIHKVPLEVYSDQEKGNRFILR
                     FRHAPISPSLPEAQALVSSPATPAVQGIGKVAILFLLTTVLLGCAHGSGELKSEQSAN
                     QVIRDWYSHFLVLNRHTDGYRPPVSARTFGYLGIAGWEAAIPKFANALPLDHQIHHLR
                     LQPFDPRKPYCLEASLNALYYNLALYFYPSTNRLLKSESKNLYESRLENFLENADSGA
                     IFPSDRYGKYIASQIYAYSNQDSLGRHAYLYNYDVSFKLPDQPGIWKPTGTDQMPPLL
                     PHWGNVRTFTPGIRSLQIAAPLPYAENQNSELFSQAWELYTLSKSLSLEKKWISEFWS
                     DDFHGVTFCAASRWISIAIQAMEKARSMPVEDRLEMLAKIGIALNDAAVLTWSVKYQY
                     NTERPETYIGRLIEPHWQPLHESPSFPSYPSGHAIFGAAAASILTNAFGESFKMADYS
                     HDHRKEFLGKPRKFNSFHEMANENALSRMYMGVHYRKDCEEGLRYGFRIGDEVNRLNF
                     RKQDKAFKE"
     gene            11534..12175
                     /locus_tag="PKM27_08980"
     CDS             11534..12175
                     /locus_tag="PKM27_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNR07435.1"
                     /translation="MSGTDQFLTVSGPASGTYKDKGSTFQAFIFPLRDESEVKMLVTR
                     LHKEHPKARHICYAYRFGPDPEYARVQDDGEPPGTAGRPILSQLKSHHLENTLIAVVR
                     YFGGTLLGTSGLIQAYKQAAADAVTRSEIITRHREAQFRILFPYTLDKPVTAWLKSIP
                     ARDLKKQYAELIEARFSVPVSMAGEFMALFQKTKAEHQWPEFPAFECLSTDAF"
     gene            complement(12172..14949)
                     /locus_tag="PKM27_08985"
     CDS             complement(12172..14949)
                     /locus_tag="PKM27_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNR07436.1"
                     /translation="MRAVAAVWIAWSFYCTSFSQVTVDFTADLTAGCEGLEVRFTNRS
                     TGPVASLRWDLGGQESAEPEPVRQFLVKGKYKICLWVTDSTGLVDSVCKPDYITILES
                     PEIQYEKEDFPQACAPLPVNYLVRHQGLVSLSVDFGDGNTESLDIAEPEVPVKHTFLS
                     GGHFFPVLRATDSFGCSSTIPIDTIVLHEVSPVLDFNSQVLCGSPAELSITDRSISTD
                     PISAVIFRVTHANLSVQYDSIPKSLQLLEAGSYSVFLKIHSGFCADSVSYPDAVRLFF
                     KPAAAYRVSETLFCRGDTMTFTDDSRSFEVIRSLEWMIGDQSYSGPVLHVPLHQGSLT
                     VSLVATTEPGCRDTAVMTFQVMESVSASLPKLLRACEGSPVSLQASLPQPVEGMGFSW
                     KYGDQTLCEYCLQYTVRMDTSRSYTFEARHPNGCLTTYDQYIDVLPNPLPDPGLQADT
                     IICRGEVVPLSAVFLEDLFSYQWDPSRPGLSCYDHCRNPIAKPDSTTTFVLQLRNAVG
                     CIREDSVRVIVNSPKGIDLGPDRIICPGDSYPFDVREVSNHRWSGSSGLSCTNCPDPV
                     ARPLNSSTYILSALEDGCPVTDTIRIGLLSVELLQGGKDTVLCSGSPVRLDASRYPTA
                     RWLPSDVLDEPDSPSPVARPISATTFFVKYTEDLCTIRDSFSVLVLLSTEITGRDRTV
                     CPGEEFSLTAEGEATRYQWTGPNISSGQNTNSIRARVREAASFQVIAQNHTCSPDTLR
                     LMVDVIPFISLPAQVSVSAIKDLPFQLNKTLVLDSHYLFQWTPASGLSCRDCTRPILL
                     PDSPRTYRFLITDPATGCMLEQTVYIQLVESCQASDFFSMPNLFTPNQDGVNDLLFVI
                     PKAADQIRSFRIFDRTGDLVFETFDLRVGWDGRLKQKEMGNGVYVYLVEAECPQLNQT
                     ILLSGDITLIR"
     gene            15105..15716
                     /gene="coaE"
                     /locus_tag="PKM27_08990"
     CDS             15105..15716
                     /gene="coaE"
                     /locus_tag="PKM27_08990"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNR07437.1"
                     /translation="MLKVGITGGIGTGKSFVAGIFRVLGIPVYEADRRAKEITEESPG
                     LKKQIIDLLGTQAYTPEGKYNRVFVSRAIFTDARLKQALESLIHPEVRKDGEAWFRRQ
                     EEEGHPYCLKEAALLYESGSDRDLDQIIVVDAPLELRIRRLIKRDGLSENEIMNRVNQ
                     QWAQEIKKEKADFIIHNDGRHPVIPQVLAIHHQLVRGASVSLG"
     gene            15730..16962
                     /gene="rocD"
                     /locus_tag="PKM27_08995"
     CDS             15730..16962
                     /gene="rocD"
                     /locus_tag="PKM27_08995"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNR07438.1"
                     /translation="MNSQDYIRLEDRYGAHNYHPLPVVLTRGLGAEVWDIEGKKYYDF
                     LSAYSAVNQGHCHPRIVAALTDQASKLTLTSRAFYNDQLGLFEQKVNQRFGYDQSLPM
                     NTGAEAVETAIKLCRKWAYEVKGIAANRAKILFFESNFHGRTLGAISASTEESSTGGF
                     GPYLPGLVILPYGDLDSAREYIESEPDLAGLIIEPIQGEAGVILPPEGYLKSLFDLCK
                     KKNILFFADEIQTGLGRTGKWLCCEHEGIRPDVVLLGKAISGGMLPVSLVLADKAIME
                     VIRPGQHGSTYGGNPLAARVASVALDVLEDEHLIENAEKLGKMLGLALADLMEDCPWL
                     VGTRGKGLFHAIEVDDDPESKTAWKICLDLARQGLLAKPTRGNIIRLAPPLVISQSQL
                     EECIGILRQVFMSSEALV"
     gene            17225..20323
                     /locus_tag="PKM27_09000"
     CDS             17225..20323
                     /locus_tag="PKM27_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR07439.1"
                     /translation="MARSILLFLFIIPVSFPLTAQKTALYSEINAHYKEGLRLFQEGV
                     YSGAKKEFQQVLEDQRLSFEPEYQTLRTYAEYYSAISSLRLKLDEADLMVTEFIKKHR
                     PDPIAEKANLEVANYYFDQKNYERALAFYNIMDTRGLSSESLSEIKFKQGYSHFAKKD
                     FKNAALALKTVSGLKTEYYFPVNYYLGMSQYFLAQYDESIKSLDIASKSKKYADLIPY
                     YITYIYFSQGKYDQLLAYTKTFLDGTQKVDRINLIRRMAGHAYFSKKDYSSAIRYLEE
                     FEKTNQSLEEADYYALGYSHYHLGQYPKAIPYFRTISSQQSQLGQNSNYYLADCYLRS
                     GDRLSARTAFYNVSKVQYQEDIREEASFNYGKLSAELGYDREGLTTLNNFPASSRYYS
                     QAQDIMADILQRTKNYEQALRTIDQMENPSARLKQAYQVLAYNRGLQLLQEDRLDEAR
                     PFFQKAIDPSFDRNIQLSAIYWQADLTHRLKDFDQSIKWADRLLSQAGSGSNLKNPEL
                     VPMARYLQGYNYLKKGNWLTAGDQFKQCISVLSQKSTGADPGLLPDALLRAADCSFKR
                     NNYDDAINGYAQVIRNKYTGNDYAQYQTAILQGLKGNNREKIRLLDDLPKKQPQSKYA
                     DESLYELGVTYTDQGQMDEANGVLKKLIRDYPGSNIYNRALIQLGLVNYNLGQKNEAL
                     THYKNVFKHNPTASESQEALVAIQEIYVDDLGLADEYIRFIESVPGYNLTNYSRDSLN
                     YSVALRYFEDGQYERATTAFSDYLQKFPNGAHSLAALFRRGESYTLLKKYDPALQDYE
                     RVIQRGRNEYYDRTLFKAASLAYHHAQDFNKAFKYYAELAGAETADESTVYEAQLGAM
                     RSAYRTGQTQDVVQFGEKVAGNPQANDEQKSTASFYLGKIAYDQKNFPKATGHLTTVT
                     RLSDNVQTAEARYLLANILFLQKQNAQAEEAVKQAIQQNADHPLWVAHCLILLSDLFV
                     EKNDLFNAKAALEAVLENYKEDPSISRITQEKLDAVNKKQQNSSKLKTDSLRSNSLFE
                     NH"
     gene            20334..21947
                     /locus_tag="PKM27_09005"
     CDS             20334..21947
                     /locus_tag="PKM27_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07440.1"
                     /translation="MKKIFLPIAVIALNFHAGAQQKLPVDVIQVVKDFDARLDETEKI
                     KISPELIALDTTKKSYQYAVNPGVLDVKYAAPQLRPVPLKPADPLPVYPFYLKAGYGM
                     PNQAYGKLSYGWNKNDRAKIGFDLSHHSANNNKKNRAQKFYDNDIKANALFITEDGMA
                     IQGDASFSKDRYHHFGHYIQNPTTETPEQILRHDYDLLQIGAGIFNPTVSKIGINYFA
                     GIKFASLADNLGSKEKNANIHLGATKWIKDRHSFGIELGSDFNTLKDSASQELNNFYL
                     LPQVGIHGKSFQISAGVRVTSHKEEINLFPAVNLSLSIAGNQLMAIAGADGGLNKNSY
                     RTLSDYNPFISSRPILTNNTFTSYFAGIKGSAKTIEYDGRIGYRQNNGLPLFILNTAN
                     FSRFDVIYRDIDLFHITASLAFRPTKHIIISSALNKNFYSDNPEPNAWGLPSIEFNGS
                     VQYQSLNQKLLFTAEAFVNDGIPYLDDLGNSGKSKVLLDISLGADYHLTKHFGLFVQA
                     NNLASNQWRRWYQYPTFGINVVGGITLKF"
     gene            complement(21988..22974)
                     /locus_tag="PKM27_09010"
     CDS             complement(21988..22974)
                     /locus_tag="PKM27_09010"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNR07441.1"
                     /translation="MSRMITLRDSLREAMIEEMRRDPLVFLMGEEVAAYNGAYKVSKG
                     MLDEFGPQRVIDTPISELGFAGIGVGAAMNGLRPIIEFMTWNFAILAFDQIVNNAAKT
                     LSQSGGQFSCPIVFRGPSGSAGQLAQQHSQTFESWMANVPGLKVISCVDPADAKGLLK
                     SAIRDQDPVCMMESEILYGLKGDVPEGEYLVPIGKAAIRRPGKDVTIVSYNKMAEVAK
                     KAAEELSKEGIEAEVIDLRTIRPLDFHTLVESVKKTNRMVVVDEAWPFSGVSAEVAYE
                     MQKYAFDYLDAPVTRVNSADTSLPYAPTLVDAWLPNPAKVIRAVKEVMYVKG"
     gene            23084..26071
                     /locus_tag="PKM27_09015"
     CDS             23084..26071
                     /locus_tag="PKM27_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR07442.1"
                     /translation="MHEEEIKDLLKKAFRLEGRLTTLYSELDAVYVLDTAEGGKLVVK
                     QSHPGRDPSILEMEDRAILYLNQQGYDFQLQEVFMAPDGDRMVRYQDHHFRVFKWIAL
                     PLWAECKPVTANTRKSLGKMMGRLSSCLAGFEDPAAHRFIKWDPSRLLWIRDFVHLHP
                     DPWNQKFIAWLNHYEKNVLPLLDSCPKQINYNDANDYNVLCRWNEEIQSYETHALLDF
                     LDMVYTHRINELAIACTYAILKLPDPLRAACEVVGAFHKACPLEEAELEVLYAQITAR
                     LMISLTVSAINRQDHPENQYLQVTDRDAWSLLEAWLNVHPRFAGYSFRAACEKKPVPG
                     MDRWIQKIGASRFHPVLDHFMDHDPYLFDFGVGSTELGNFSVFTDDLRLDKQVRRILE
                     DHGTSRGIGRYKEIRPFYTSEAFRREGNEGPDWRTVHLGLDLFAPAGTVVRAPCSGRV
                     HSIRNNGGHRDYGPTLILEHYLEEFVFYTLYGHLGLELFSGLDRGQAIPAGFEIGKIG
                     NIHENGGWPPHLHFQIILDLLDHEGDYPGVASPGELIVWEALSPDPALITGIFSLREE
                     EPSLTEMLRLRKERLGPNLSVSYREPLWIRRGMMQYLLDQHGRRYLDTVNNVAHCGHE
                     NPRIVDKGRKAMAVLNTNTRYLHENILGLAEQLTAALAPSLRVCYFTNSGTEANELAI
                     RMAKQYAGQEDILAMQWAYHGNSSLMIDLSSYKFNRKGGRGKPPHTHLIPMPDPFRGK
                     YAGRMDQAEAYFKEVENIINGLKEQGHGPAALFAESILSCGGQIVYPDGFLDRAVAMV
                     RAAGGLYIADEVQTGLWRTGRFCAYEFYGVVPDIVTFGKPLGNGHPVGAVVCTRPVAE
                     AFHNGMEYFNTFGGNPVSCAIAGEVLRELMDQKSMDRAKEIGDSLKAGLKNIQTRYPL
                     IADIRGHGFFLGIELMKNGLPASAGASYLAGRMRQYGILMSTDGPDDNVLKFKPPMCF
                     TGDDATQLLDRLEKILAEDPLRI"
     gene            complement(26088..26528)
                     /locus_tag="PKM27_09020"
     CDS             complement(26088..26528)
                     /locus_tag="PKM27_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07443.1"
                     /translation="MKKIYSRSGHKTYETPLLGKIGIQPGQQILLVQAPDFILFLFRN
                     GNIPVDVKAIGRKKYDLVWGFINKRDDLEARLPGLKTRLKSTGMIWISWFKKSSKQPT
                     ELTEDLIRDAALRNNLVDIKVASVDENWSALKLVIPLNKRDNTA"
     gene            complement(26488..27273)
                     /locus_tag="PKM27_09025"
     CDS             complement(26488..27273)
                     /locus_tag="PKM27_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR07444.1"
                     /translation="MGQYRIFQSCFWQLNSVVYHENKTVLCWDPAYSAAEIERIRAAM
                     DPSGIVDHYLLYTHGDYDHIAGDSAFTGPLRVGSFGMAERRDKAEALGLIEAVDHEYY
                     VRRDFPPRFPKLDIKVEDKGPSTQRLSDVEVHFFPAPGHTADGLFTLIPSMGLWIAGD
                     YLSDIEFPFVTDLGAYAQTLETASRILEDFPIEVMVPGHGNVALRRAEILDRIERSKA
                     YLGSLEGSERIKPWQESWGTSPFYSFLDKMHEKNIQQVRSQNL"
     gene            27605..32650
                     /locus_tag="PKM27_09030"
     CDS             27605..32650
                     /locus_tag="PKM27_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07445.1"
                     /translation="MKKTITFLCLKRCWLPVVLALFYSLSMSAQADTCNLVCNDHVNA
                     SMPADGCLKTFVPDDFLENPGLTCTTYDVELSYPYGTAKLPGGDQVNRSHIGYTFIYR
                     VIETVSRNSCWGYVTIEDKAAPQPVCKNVRVSCFQVARLSEIVGEIIDNCGQAGSSAI
                     ESMQWSDWGCDSVSGLGRVIRTIRTWDDWGNSATCSDTLTIGRDSLSNVTCPDPIVLG
                     CRVNCQGKGITLISKDKNSPNYPSPDFLLDLQKRDTAGGGRKCVPAGLKVVPFIDDSV
                     LVMQGSVCILVDSSVAMYPAPGGFCKTSLTWTDQIIPICGTGFKIRREWLIVDWCNRT
                     DTTCVQYIKVEDSEDPIVKSNSKFYYKANTSPHNCYANINLDALIVDDCDVNAKQSYI
                     AQYTLESHPAKINVINGTLPGKISLPAIPSDYKGKAFHRLEVSIVDACLNRTDTAIYV
                     KVSDVTPPNPVCDEYTRTTVDPSTCWARVYAADLDNGSRDNCCNVLHFAVAHMDTIEA
                     ARNAWKSYWTANCNSTYWKYKDRWDDWLEAWINVFIFKDYIDLSECGENQVVLRVYEA
                     CGVPLYDPHVFPCSKHDWFTYNSFKLSRLWHNYKFFYLNDKTSPNYKNCTSSFGPFCK
                     PTHWDPPSVDYSGRYPGSETYVRSCEFEFGDEESLPSATNNFGGLEPGNTCSARLYND
                     CMVIIYVDDKTPPVCEEPADLYWYCDNVADAREIKGKKYFIRGHRYNRLEYAHAVCDI
                     DYHNDNILASGNGKYDYDPTLHADDGSCRYDYTPYNEIECEEEFDGDLTDAVDATGKA
                     FGWYGCNIYGPAHQDEHGYYKEICEDDHDNWAPIYCHTWLCLDRKDSGTKVDPKTAFW
                     TPVLRSGGRGADVDEGQFIIWDNCWIGTITSVDESYVDNCGNGWLKRTWTTTDKCGSP
                     VVCDQKIITKHRSDFEVEFPADLTVSCDVEGSLSPDGTGRPIIMDDECELVGVNYEDV
                     RYDIVPDACYKIVRTWKLIDWCKYDPNAHYRKPEVIVDDRKVAHPTDRYCVYRHLKDE
                     GDGYITYTQIIKVIDTVAPVITCTDTTICLYTGTDANCTAPNLSVPFIATDNCTASDK
                     ISFRWELDLNASASDVANHKYNKASIDDKSYTPVTAFSSNTIKDGTHLVHVIASDNCG
                     NVDTCTFVLTVKDCKEPTPYCHNGIATVIMPSTGEITIWASDLDAGSYDNCTPKSNLV
                     FSFGPDKSVTSKVFTCADIPNGTSNTIEVDIYVWDAYDNKDYCRTYLLLQDGIGNKCT
                     DNLENAGSIAGKISTENDDAVEKVKMNVKAPQNMPAYLTDVKGQYSFGGLPMKADYSI
                     IPARNDDPMNGVSTIDLVLIQKHILGSSPLNSPYKIIAADVDKNTDITVLDLVELRKL
                     ILAVYDELPNNQSWRFVPKTYQFEAGKNPLKTSFPEQIDISKLSQNELNRDFVGIKIG
                     DVNTTATPHSLLGAEAREASATLQFRTEDRQLKAGETATIDFRADNFSGIEGYQFSLA
                     LHGLTLVDIQKGSLEVDASNFGLTKLNQGYLTTSWSKEKGARLNPNDVAFSLKVKATK
                     DVTLSESLKINSRFTRAEAYAEDGNNTKYLGVSLEIGKSSRPSNYVLYQNTPNPFKAQ
                     TLIGFELAKNEAVTLSVTDVTGKLIKSYQIDGVKGANQLVVNRNEFGSAGVLYYSIKT
                     KSFSDTKKMLLVE"
     gene            32962..>36593
                     /locus_tag="PKM27_09035"
     CDS             32962..>36593
                     /locus_tag="PKM27_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07446.1"
                     /translation="MKSLFRFCLIVSVCAGFFTPPAHAQCNLICNDHIHASLPGTGCN
                     RTFDADDFLENPDPSCAYTVKLTYPFGTAKLNGTEVNRSHIGYTMIYSVSAPGNSCWG
                     FLTVEDKAPPQPFCQDAVISCFQLARYNELVEKIVDNCAEDGKSIIESLRWEELGCTS
                     PVGIGHVIRKIRSFDTWGNSGTCIDTLTIRRDSFELIKCPDMVTLNCLVRCKKPNNDG
                     ANNDPKNFDLIYFSSNSSSPYYPTPEKLLDLQKRDTFNSGDRKCISPLLKVVPFIKDS
                     VFNSEGELIDTCVNMYPSGAVLCKIVFYYTDLVFPICGTGFKIRREWRFTDWCTGEDT
                     VCIQYINILDKQAPVVKNSFSIPVQIKDTLYDFVFPGYRRDLVTNTHDCNRTFCLDSL
                     TTEDCSKVTQTYEFTYTDPNGGNSIVHRTGGPKDCFTLPGAPNNYLFSGIVMGTPLEH
                     YKEISWPRNCYPLFIQTRDECFNTTAGVSKLINRKTGQLVAENPLNFAGIILVCISDE
                     TPPNPVCDEYTQTTLDPEQCWSRIYAKDLDNGSRDNCCELLHFAVAHMDSITHYRERY
                     AQQIKDSCGTAEYDKHRKKYAEIIENWINCYVFNDYVDLTDCNENELVLRVYEACGVP
                     RYDEHIYSCSPHSWFCYNTYPSYMLWHNYILDTDPARKCLTPLPWLCLDKQWKLISHL
                     IYKSGKDYWRPKYAGATQLIPDQELLYGPAVCYPEFYNPAQKAVSEPAYAPGNTCSKR
                     LYSDCMVTVFVDDKTPPVAEKPDDLFLYCDNSAREQKYSNEYAKCDDDSWTPDNGKDL
                     RCQDQKYKPYNAIESIVENDSDQGDTIDPTKKAYGWYGCNIYGASHPDEHGEIAPCIP
                     EANTWFPIYCRSWLLKDSSDTGARVDPLTQFSQPVLNDGNPGNASPGAGKFWIWDNCW
                     MNDSSLTYTDESYFDKCNNGWVKRTWTARDKCGNTVSVDQKIVTKHRSDFEVVFPADK
                     ITVCDLGEDVSPDAIGRPMIMDDECELVGVNFEDQRFDIVPDACYKIIRTWKIIDWCK
                     FDPSQHSRDQDIIVDDRLVADEVKRPCVYRNLKDNGDGFMTYTQIILIRDTIPPLATC
                     RDTTVCIYDENCALPSIYIPFTATDNCTAPSLISYRWEIDENPSPADTVAKRYNKSSI
                     DKTSSGPQQALSIVQPQGISLVHVIAEDNCGNEDTCTFILRVRDCKKPTPYCYHGVAT
                     VIMPTTG"
BASE COUNT         9488 a         9635 c         8911 g         8559 t
ORIGIN      
        1 cgggtaaggt ataaatgcgg attcctcaaa ccggaaccct ccctgattca agaatatatg
       61 aatcccgtgg taaggcttgg gaacctggct gtaatccgca ttatccccgt ttgagatcag
      121 gatatcgacc cttccgtctc cgttaaaatc gatcagatcc aaatcacaga gcccatatac
      181 cggaggaaag ctcaaaagcc tttggtaccg cggggcgccc ccggccaccg gcaaccaggc
      241 cgaaacctgt tcgtctccct gggcgataag gcaaaccagt tcctttcggc tatccccatc
      301 cagatcgacc gcttcgcttt ttatactgcc tgatcctgaa aacaagctga ccgcctttgg
      361 gtcctttcgc gaggaaggat accaggatag gcggcctgtt tcatacccaa actcatggat
      421 gatataatca gactcagcgt ctccgtccag gtccatcagg ctgagccaga ccggacgatg
      481 taatccgttg atctcctcct gccaactgcc gcccttccgg gtgagcaggg aaccgaaaga
      541 ctgttccgat ggatagagga tgccgaccga aaggatacgg gtcccttctt ccccggggat
      601 taattggatt ggagtcgatc cgatcgcgaa gctgtccgaa aggtcccagg aggaatccaa
      661 aacccggatt tgacccgatt ccaatccggc gaacaccagg cccgaaaagg tgtccacggc
      721 aagcatactt agccggggta ccctcaccag ggcgcctttc ggatacacta cgaattgttc
      781 cagatccacg gaagagtccc gggggacgat cagcgtatcc ggcgcattgg agatataata
      841 atccctgatt ttaaaccagg tggaatccgg aatcaatgga agggcaggat aaatatttgc
      901 ctgctccaaa agattttgct cgagcatatc ctttccttta taaggactat acccttccgt
      961 gcggatcccc agccggcatc ccatattggg caaaagacta tgttcccagg tttttttatc
     1021 caaaaggcct ggttccggga accggtgaca ggaaccacaa tattttgttg caagcgaaag
     1081 ggtatcgagt gcttcggttg ccaatccggg cccattattt aggcagcaac cctgggccaa
     1141 caggaccagt attaatatcg cggcccagca atccgacagg acccatttaa atgctgtgat
     1201 cagatggtag atgaaggcaa aattacacaa tccggcattt tctgatctca gtgattccaa
     1261 ctgcctggaa gcagttccat ttattcgcca ggtattattt ttagccaatg actgctccat
     1321 ccgtcgccct cacgtttacc tcggatccgg aaatcctgga ggacgtttta accctgcaac
     1381 gcctgaatct gcggaaattc ctgagccccg aagaaagcgc cgcccagggt tttttatatg
     1441 tggaacacga cccggtaacg cttaaaagca tttgccggtc ggaaccggcg gtagtagccc
     1501 tggatggcaa gcggctggcg gcctatgccc tttgcatgac ccgggaacat ggccattccg
     1561 tacctgaatt gtttgatttt ttcaacctgg tggattcgct tcatgttgag gggcgggccc
     1621 tggcggacca tgggtttttg gtttgcggcc aggtctgcgt cgccaaagaa taccgtggcc
     1681 agggcctcat gacttcgctt tatcataaga tgtcggaaca ccggtcgaaa tattattatt
     1741 gtgtcactga gatctcatcc cggaataccc gatctttgca tgcacattcc aaggttggtt
     1801 ttgtacccat ccgggaacac cggcacgcag atggcgaatc ctggcaaatc gtactctggg
     1861 actggaacaa aatcaattaa acctggagaa ccacaaatcc ggaaatccaa tatgagcaaa
     1921 cgaacgaatc ttgttgaatc cgccctcccg gaattgccgt ccatggccgg gtaccggttc
     1981 tgtatcatca gcacggcctg gaacagggag atcaccgaaa aactcgaggt agccgccatc
     2041 aaaaccttcg tcaggcacca ggtaaaaaaa caggatctcg aagtgatccg ggtgccgggc
     2101 tcctttgagt tggtattcgc cgccaagagg atcattaaaa agtccaggcc ggatgccgtc
     2161 atctgcctgg gatgcgtagt caggggggac accccccacg acgtctatat ttcgcaagcg
     2221 gtatcccaag gcatcatgca actcaatatc ctgaggaaca cccctgtgat attcggcctt
     2281 ttaaccaccc tcgaccaaca acaggcgctt gagcgggccg ggggcaaaga aggcaataaa
     2341 ggcacggaag ccgcgatcac cgccatgcat atggcccttc tcaacgattg atgatggtcc
     2401 tcaaatcagt gcttgccggg acttttaaac tggatggcgg ggccatgttc ggggtagtgc
     2461 ctaaacggat ctggagtaaa ctcatgcagc ccgacgccaa taatttatgc gactgggtca
     2521 tgcgctgcct ttatgtggaa gaccgccacc accgcatcct gatcgatacg gggatgggaa
     2581 acaagcagga tacaaaattt ttttcctatt acgaacctgg gggcttccgg acgctgcagg
     2641 cggccttgaa tcaccacctg atcgacccgg aatccattac cgatgtaatc ctcacgcatc
     2701 ttcattttga ccatgttggc ggggctttat tttacgatga ggccgggaat ccggcagcaa
     2761 gtctgccttc ggccacccac tggacccata aagaacaatg ggacctggcg gtaaagccca
     2821 atgcccggga aaaggcttcc ttcctgccgg aaaatttcat gcccctgctg gcccaggaca
     2881 ggatccgttt tttgcaggat ggggattctc cctttccatc gatcgagttt atccgtgttg
     2941 atgggcatac cagggctatg gttctgccct tgatccatgc gggaccggat aaaaaactct
     3001 tgtttacggc ggatttattt ccttcggtac accaccttgc cctgccttat atcatggcct
     3061 atgatatgca gcccctcgtc accctggccg aaaaagagct tctgttgaac cgttgcatcg
     3121 aggaaaagat tgacttgtat ttcgaacacg atatagatca tgaggtggcc tccctgaaaa
     3181 gaaccgaaac gggaaaagtg gtggccgccg ccttcaccac cctggaggaa tggggtgtca
     3241 aaaccggata aactggtcca gtaacctggt aatggcattc cgggtatcct ggtcagcaag
     3301 gatggattgt tcgtctatca ggtgttcgat ccgggaaacc ggaaggcggt tgggatatac
     3361 gatcatattg agatacatca ggatcccggt cagatgttcc attccacgca ggttccccgc
     3421 ccggcctgaa gcaaccccaa tcaggagggc tttcttatga taaaaagctt tttttgaatc
     3481 cgccacggag caggcatcga taaataattt caggataccg ggtatgctgc cgttgtactc
     3541 ctgcgaaatg aagaccattt ttgatgccgg aaagacataa cggttttgga tgtccatcag
     3601 cctggaggaa tggcggtcgg cctgatacat ggattcggaa tcaaaggctt cgatagggat
     3661 ctcggcgaga tcgagacttt ctacttcttc cccgctggtt tcattaaggt atttggtgac
     3721 aaagtctgta agcggtttgc tcaggctccc ccggcgattg gtcccatgga cgatcagaat
     3781 catacgaagc aattaacaat cccagggcct taaaggttga aagccgggaa tggaaatccc
     3841 aggggaatca tgcgctttaa ttcctcccac actccccctt gcccagatca agggacgccc
     3901 aaggccggga tgcgagcaga acttgcccat ttgcccactg tctaacttac attaaagatt
     3961 cagggaacta taaccgaatg ccgaaggaaa caaccaggct ccttccgtca gaactccaga
     4021 ccccctggcc cggataaccc gcaggccttt tggtaaagta ttgccggttg gccaggttgt
     4081 ttatccccag tttcaacaga tagtttgcat taaaacgata agacatgttc aggtccagga
     4141 tcgtataaga gggaaccctg ccctttgcac cgtttgcgct gggctggacg gtatttacgg
     4201 cgtcggaaaa gatatcgtcg acataactgt gctggaagac cgcggtgaag agtttgtagt
     4261 taaattgaat tccattgcgg ctgatccatt cgggcaccgt ttcaagacgg ttaccttcca
     4321 gggatttgtt ttcattgcct tccctcaatt tgccattgag ataaatggcg tcaaaataag
     4381 acgaagccgt gaagacagac aatctgtggg tggcttgatc tatcaggttc cactccgcat
     4441 aggcctctat cccatgggtc cggctgttcc cgatattggt tttgaggaaa tacgtctcac
     4501 cggcaccctc tgtgagcaca tgggtaccta tccggttttt ataatatatc cggaacaggc
     4561 tgaggtcata tttcaacttc gcactgattt gacctttgat ccccaattcc gcattgtgac
     4621 cataagcatc cttcaggtcc ggatcagacc ggtcgaggac cgttgcaggg atgatatcgg
     4681 caaaaataac gggccggtag gcctgggacc agcctccgta aaccaggtga ttcgagttca
     4741 accggtattg accgctgatg ccaaccaggg ggaaccggtg atcaatatcc tgtggcactt
     4801 tttcatccgg caagtaagaa atgatccccc gcatcttgct ggtcccgctt tcaagcctca
     4861 atcccacaga caagtccaat ctcggcgaca gttgaaacaa attctcagca aagaaagccg
     4921 tattatccgt tttgaaatgg acatcacgcc caaaggacga ctcggtgatc tccaggttgt
     4981 aatccgtacc ggtggtccct ttgccctgtt gtctccggtg taagtcgtta ttgaccaatc
     5041 tcagtccgcc taccaggatg cctcctacct ttccgagccg gtaatgttgt tgcaggcgca
     5101 gttcactgga ataactgttg aaatgatcga tatcaacctg ccttggttta tactgtccgg
     5161 tcagcggatc aattttatcc tctgtatccg caagaccgat aaattgcaca ctgtttcggg
     5221 tacccaggac agccgaattt acccagttca accgggtggt cgggctgatt ttccaatcca
     5281 gcgaaagaga aggaatatat atatccgggc tgaaataatt tcttcggcgg gtggattgtc
     5341 tgggattatc ccgaaacatg gcgtccgaca gggggccggg gatctggtac aggtattggc
     5401 ttctgcccag ttcagctttg atgttgaaat cgtgactggc ggcataagtt agattaaaaa
     5461 actgggactg ggcatccgag cgggagtcgt cccggtatcc ctttgaattc cgccgatggt
     5521 aataggcata ataccggaac ctgccgcttt tgccgccgat cgactgatag gtactcagca
     5581 agccgtagga accgatggaa ctgaggcttt cgtaactgag ggctttggtg gtatcccctt
     5641 gtttggtgac ataattgatc atgcccccga actgggaacc atactggagg ctggaagtcc
     5701 ctgaaatcat ctcaatccgg gaaatggatt ccatgggcgg gctgtaatgg cttgccggat
     5761 aaccgtagat atccgaattg gtcatgatat tgttttgacg gacattgtat tcccagctcc
     5821 ggtgcgggtc cagaccccgg ttggatacgt ttacctggtt cccggaaccg tccatatcgt
     5881 aaacaaagat tcccggcttt tttgcaaaga tctgccttcc ggttttttcc gccagattgg
     5941 ccggcagatc ctggaccgta agcacttcat tttttttacc ggaagtgata tacgtttggt
     6001 ggaccgcttc aagttgccgg acggctccgg cttcgaaacg gtagccctgg atctctattt
     6061 tcgagatctc cttcaccatc agagtatctt cattttggga ataaagtccg gtgcatccga
     6121 accacaatgt ccatacgatg atctttttca tattttgatc cttcgaatcc tgtgctgaaa
     6181 tccaacggaa ttatacaatt ccacccctct tttggtgccg gaaatgttat atacttcctg
     6241 cgcaagtcta cataaaaacc aacaggtatt ttttaaagat ctgataagaa aaccaggtga
     6301 ttctaaggac tttataagat attgggaatt aaaaaaggtg tgagacaacc ccacaccttt
     6361 tttaatggtt ccggaaccgt ttcagtccgg taaaatcatt cagcccttta ttttttccag
     6421 ggtaatttca gtacccttct ggaaatagcc tggccgagag caacgcctgc cacacagtcc
     6481 atcctgaaat gcacacccag ggtcaccctg gagagcgcgt tttcataacc ggcgtcttca
     6541 aaggaattga aggtccgggg aattcccaga aattccgtgc ggtctttatg gcagaggtcg
     6601 gtgaattcat aattctgtcc gaacaggtct gccaacagaa cgcccgcacc cccaccgaat
     6661 gtggagtgac ccgatggata agcaggaaag gagggagtga tcccctgaac cccgctcacg
     6721 gtgtttacaa ggatcggttc ccagtttgga tcaatgagcc tcttgatata ggtttcaggc
     6781 ctttccacat tgtagacata tttggagtgc cagcaggcca ccccataatc gttaaggccc
     6841 aagcccagtt tggcaacgga ctccactgct ttttccagat tggccttttc tatgacaagg
     6901 acctggtcga ggatggcaat aacccgggta ggaggactga aggtctggcc aaaaatatca
     6961 tcgctccaga actcagaaac ccatcggtcc tcatatctca tgtccggatt agaaaggctg
     7021 tacacttcaa gggcttgtgc ataaaacgga gaggttttgc tttcgctgta tgccagcgga
     7081 gggggacaca acttctgggc atcgttggtc acgaaagttc tcatccggcc gccataagga
     7141 aacatgcctc cgtcggggtt atcgttggtg ggttgccatc tgccgggaac cactttacca
     7201 tcgaccatgc tttttgccct ccagttgtaa taaaatgcat ctcctttggt gggatcgttg
     7261 ctgaggggat tcaaatcgga ttcatgacca taggcgtcgg tgatagccca gttccatatc
     7321 gccgtagcca ccttttcgcc ccaggctttg gaatcgttaa aaagtaccag gtcattgatt
     7381 tctgctttga atttggtttc aagacctgtt tccagggttg ctatcatttt ttgggcttcc
     7441 gcattactga tgtgcccgaa tccctggaca aaattcgtct tgaaaaagaa ttttttcatc
     7501 aggaaagcat agctggagtt gatggccgta ggccaatgga tattctgccc aacctggaaa
     7561 tcgggaattt ttaagtcagt gccccactgg gttttcaaac tgttgaaagt aggcatgccg
     7621 ggcacggcga tttcatatgc tgagagaccc atataggcga gcgccctggg gcctgggcag
     7681 ggcctgtagc caagggcatc cttatcgatc cgggcaaaga gttcattcca ctcccagatc
     7741 acggagtggt cataatcggc gaccagattg gtttccggca cttcttcacc gggttcttcg
     7801 atggtgtttt tctggcaact ggctaccagg attaacaagg taaacgggag gcaaagtctt
     7861 agtaaattaa tgttcataag agttgaatta gattgaagat aagcaagaat ctagctttct
     7921 tgcggatcaa atgtaaaaat agggctggaa tccacaaact gatttgggtc ataatttttc
     7981 ttaagatttt gcttagaatc ctctgaacca cagatttaca agccaaaact ggctaaagat
     8041 cctgatcttt gggcagatga aaatcctgct gatcgaagat gagcccaaaa ccgcgctatc
     8101 catccaggcc ttcctgaatg aaaacgggat tgagaccgat ttatgcctca acgccgagga
     8161 agggctccgg aaaattgacc gctccccctt tgatgtcatc gtcacggatg taatcatgcc
     8221 ggatatgaat ggtatagagt tgtcgcggaa attgagatca aaacagatca aaacgcccat
     8281 cctgatgatt tccgccctgg atgagaccga tgacaaggtc aagggcctcg attcgggcgc
     8341 agacgactat ctcgccaaac cctttgacct gaaagaattg tttgcaaggg tctccgccct
     8401 gcaccggagg tccaaccttt caaacctctc cgcggaaaaa ctgaattatg aagaccttga
     8461 gatcaacctg tccaccctgg aagtgagccg ggccggcagg aaaattatac tcaccccgaa
     8521 ggaattctcc ctgctggaat acctgatccg gaaccagggc cgggtgatcc cgaagctcga
     8581 aatccttgaa aaagtctgga acctgggcgt ggagatcaat accaatgtca ttgaagtcta
     8641 tgtaaattat cttcgtaaaa aaattgaccg ggattttacc gacaaactga tccatactca
     8701 ttttggtata gggtactaca tcaaaaaaga gtgagggggc ctatgaaaat caggacaaaa
     8761 ttatcctacc agtttatcct tatcaccatc ggcatctatg gcctgtccat gctcttcatc
     8821 tacaggcaat tcaaggccca tatcgaagag gagatcttca gcctgctgga aagcaaggcc
     8881 aggatgaccg cggaaatggt cttgtttcat gaggacgagc ttcaacccct gcccgataaa
     8941 aaggaaccgg gagaaatatt aatccaggat gtaggcaaca cctccatcta caacaacgac
     9001 ctcaaccgca tttattccct catttcctcc gctcccaata ctccggtttc ggccctgcgg
     9061 cagatccggg aaaccgggga ttaccgtttt atcaacccgc cctacaaagc ctatggattt
     9121 ttgtacaagt caaggcaaaa caaggactat atcgtggtct ccgaggacaa gccggattat
     9181 tccaaactgg tcaaattgcg caatattctc ctcatcagca ccctgtttac cgtcctcatc
     9241 gtcacatttg gaggctggtt ttttgccggc caatctctcc ggccggtctc caggatcatc
     9301 catgaagtga aacatatcct gcccacggac ctgagcaaaa ggctcaaacc ggacaggcac
     9361 aacgacgaac tcaccaacct gatcgccacc tttaactccc tcctcgaacg gatcgaccag
     9421 gctttccgaa ccgaaaagag ttttatctcc aacgtcagcc acgaactgcg gaatcccctg
     9481 gcagccatcc gaacccaaat acaatatgcc ataaataaaa aaagaacgcc agaagaatac
     9541 aaccagatcc tttcctccct tcaggaagac atagacgaaa tgacccagac cattgagaaa
     9601 ctgctacaac tggcccgggt aaactcgaac gccaatcata ttcccctcac cgcggtgcgt
     9661 ttggatgaat tgatctacca ggcccaggaa agcctgtaca agtccaatcc ttcctataac
     9721 attaaaattg aaatgatgca tcttccggtc gaggaggaag gcatgtttgt ccaggccaat
     9781 gagcccctgc tgaaactggc cttcatgaac ctgatggaaa acgggtgcaa attctccaac
     9841 gattcctcga cccggaccat tctggatttt tccgatccgg aggagatccg actggaattt
     9901 cagaactccg gaacagccat cagtccggac gaaatcacca aaatatttca gcctttttac
     9961 cgaactacgg aacataagca ccgtaagggc tccgggatag gcctatccct ggtaaaaagc
    10021 atcctggaca tccataaggt tcctttggaa gtttattcgg accaggagaa aggcaaccgg
    10081 tttatccttc gtttcaggca tgccccaatc agtccctcct tgccggaagc ccaagccctc
    10141 gtttccagtc ctgccacccc ggcggtccag ggcattggta aggtcgcaat ccttttttta
    10201 ttaactaccg tacttttagg atgtgcccat ggatccgggg aactgaaatc agaacaaagt
    10261 gccaaccagg tcatccggga ctggtatagt cattttctcg tcctaaaccg ccataccgac
    10321 ggttaccgtc cgccggtgag cgcgaggacc tttggatacc tgggcattgc cggatgggag
    10381 gccgcgatcc ccaaattcgc aaatgccctc cccttagatc atcagatcca tcatcttcgt
    10441 ctccagccgt ttgatcccag gaaaccctac tgccttgaag ccagtctcaa cgccctctat
    10501 tacaatcttg ccctttattt ttatcccagt accaaccgtc tgttgaaatc ggagagcaaa
    10561 aatctgtatg aatccaggct tgaaaatttt ttggaaaatg cggacagcgg agcgattttc
    10621 ccttcagacc ggtatggcaa gtatattgcc agccagatct acgcttattc caatcaagat
    10681 tccctgggcc gccatgcgta tttgtataat tatgatgttt cctttaaact tccggaccag
    10741 cccggcatct ggaagccgac cggtacagat cagatgcctc ccctcctccc gcactggggc
    10801 aatgtgagga cttttacccc cggaatccgc agcctgcaaa tcgctgcacc cctgccctat
    10861 gcggaaaatc agaactcaga attgttttca caggcctggg aattatatac cctatccaaa
    10921 tccctttccc ttgaaaaaaa atggatttcc gaattctggt ctgatgattt tcacggagtg
    10981 acgttttgcg ccgcctcgcg atggatatcc atcgcgatac aagccatgga aaaggccagg
    11041 tccatgccgg tcgaggaccg gctggaaatg ctggctaaaa tcggcattgc ccttaatgat
    11101 gcagcggtat tgacctggag tgttaaatat caatacaata cggaaagacc cgaaacttat
    11161 atcgggcggc tgatcgaacc ccattggcaa cccctccatg aaagcccctc ctttccaagt
    11221 tacccatccg gccatgctat ttttggcgcc gctgccgcca gtattttaac gaatgcattt
    11281 ggagaatcct ttaaaatggc ggattacagc catgaccacc gcaaagaatt tttggggaaa
    11341 ccccgaaaat tcaattcctt tcacgaaatg gccaacgaaa acgcactctc cagaatgtat
    11401 atgggagtgc attaccgcaa agattgtgaa gaaggcctgc gttatgggtt ccggatcggg
    11461 gatgaggtca accggctgaa tttcagaaaa caggataagg cctttaaaga ataacagttc
    11521 ccctcaaggc cccatgtctg gaacagatca atttttaacg gtatccggac cggcttccgg
    11581 gacttataag gataaaggca gcactttcca agcttttatc tttcccctgc gagacgagtc
    11641 cgaggtcaag atgctggtaa cccgtcttca taaagaacat cccaaagccc gtcatatttg
    11701 ttatgcttat cgctttgggc ccgatcctga atatgcccgg gtacaggatg acggagaacc
    11761 accgggtacc gcgggccgac ccattctctc gcagttgaaa tcacatcatt tggaaaatac
    11821 cctgatcgcg gtggtccgat atttcggagg caccctgttg gggacctcag gtttgattca
    11881 ggcatacaag caggcagccg cagatgccgt gacccggtcc gaaatcatca cacgtcatcg
    11941 ggaggctcaa ttcagaatcc tctttccata tacattagac aaaccggtca cggcctggtt
    12001 gaaatccata ccggccaggg acctcaaaaa acaatatgct gaactcattg aggcccggtt
    12061 ttccgtgccg gtctccatgg ccggagaatt catggctttg tttcaaaaga ccaaagcaga
    12121 acatcaatgg cctgaatttc ctgcttttga atgtttatcc accgatgcct tttagcggat
    12181 gagcgtgata tcccccgaaa gcagaatggt ctgattcaat tgcgggcatt cggcttcaac
    12241 caggtagaca taaaccccat tgcccatttc cttttgtttt agccttccat cccagcccac
    12301 gcggagatca aaggtttcga agaccaggtc ccccgtccgg tcaaagatgc ggaaggaacg
    12361 gatctggtct gccgctttgg gaatcacgaa taaaagatca ttgaccccat cctggttggg
    12421 ggtgaaaaga ttcggcatgc tgaaaaaatc actggcctgg cagctctcca ccaattgaat
    12481 gtacaccgtt tgttccaaca tacagccggt tgccggatca gtaatcaaaa accggtaggt
    12541 cctggggctg tccggcaata aaataggcct ggtacaatcc ctgcacgaca agcccgaagc
    12601 cggggtccac tggaaaaggt aatggctgtc caggaccagg gttttgttga gctgaaatgg
    12661 caggtctttg atggcgctca cggaaacctg ggctgggagg ctgataaatg ggatgacatc
    12721 caccatgagt cgcaaggtat cgggtgagca ggtatggttt tgagcaatga cctggaagga
    12781 agccgcctcc cgcacccggg cgcgtatgct gttggtattc tggcccgatg atatattcgg
    12841 acccgtccat tgataccggg tcgcttcgcc ttccgcagtc agtgaaaatt cttctcccgg
    12901 acaaaccgtc cggtccctgc cggtaatctc tgtggacaat aaaaccagta ccgagaacga
    12961 atccctgatc gtacacaggt cctcggtgta ttttacaaaa aaggtcgtgg cgctgattgg
    13021 ccttgcaaca ggggaagggc tgtccggttc atccaggaca tcggaaggga gccaacgtgc
    13081 cgtggggtaa cgggatgcat ccaacctcac cggggatcct gaacacagga ccgtatcttt
    13141 tcccccctgc aacaattcca cagacaggag tccaatcctt atggtatctg tcaccgggca
    13201 gccgtcttcc agggcgctga gaatataagt ggatgaattc agcggacggg ccaccggatc
    13261 ggggcaattg gtgcaggaca gaccggatga acccgaccag cggtgattgg atacctcccg
    13321 gacatcaaat ggataggaat cacccgggca gatgatcctg tccgggccaa gatctattcc
    13381 cttgggtgaa ttgacaatga ccctcaccga atcctcccgg atacaaccca cggcattgcg
    13441 gagctgtaat acaaaagtgg tggtcgagtc tggtttagcg atcggattcc ggcaatggtc
    13501 ataacaggat aagcctggcc tggacgggtc ccattgataa ctaaaaaggt cttccaaaaa
    13561 aacggcggag agaggcacca cttcccccct gcagataatg gtatcggcct gcagtcccgg
    13621 gtccgggagc gggtttggaa gtacatcgat gtactgatca taagtggtaa ggcaaccatt
    13681 gggatgcctg gcttcaaagg tgtatgacct ggaagtatcc atcctcaccg tatattgaag
    13741 acaatattca caaagggtct ggtccccgta tttccaggag aaacccatcc cttcaacagg
    13801 ttgtggaagg gaggcctgga ggcttaccgg actgccttca caggcgcgca gcagcttagg
    13861 caagctggca ctgaccgact ccatgacttg aaaggtcatg accgcggtgt cgcggcatcc
    13921 cggctccgtg gttgcaacca ggctgaccgt gagagagccc tggtgaaggg gcacgtgaag
    13981 cacgggaccg gagtacgact gatcccctat catccattcc agggaccgga tcacctcaaa
    14041 tgaccggctg tcatccgtaa aagtcatggt atccccccgg caaaataaag tctcggaaac
    14101 cctataagcc gcagccggct tgaaaaaaag gcgcacggca tccggatagg acaccgaatc
    14161 cgcgcagaac ccggaatgta ttttcagaaa taccgaataa ctgcccgcct ccaacaactg
    14221 cagactttta gggattgaat catactgaac agagaggttg gcatgagtga cccggaatat
    14281 cactgcagat ataggatcgg tggagatcga tcggtcggta atgcttaatt cggccgggga
    14341 accgcacaac acctgcgaat taaaatcaag cacagggctt acctcgtgca ggacaatcgt
    14401 atcaatgggt attgtcgaac tgcatccaaa cgaatcggta gcccttagca ccggaaagaa
    14461 atggccccct gataaaaagg tgtgttttac cggtacttcc ggttcagcaa tatccaggga
    14521 ttccgtattc ccatccccaa aatccaccga aagggatacc aggccctgat ggcggaccag
    14581 gtaattcacc ggtaatgggg cgcaggcctg tggaaagtct tctttttcat actggatctc
    14641 cgggctttcc aggatcgtga tatagtccgg tttgcacact gaatccacca aaccggtact
    14701 gtcggtcacc caaaggcaga tcttgtactt gcctttcacc aaaaattgcc ttaccggttc
    14761 cggttcagca gattcctggc cgccgagatc ccaccgcaac gatgccaccg gtccggtaga
    14821 acggtttgta aaccggacct ccagtccttc acagccagca gtaagatccg ctgtgaaatc
    14881 aaccgttacc tgggaaaatg aagtgcagta aaaactccac gcaatccaca ccgccgccac
    14941 agccctcatt cagaatgttg ttttccgcta ttaaaagatt atgaaaggaa ttttagggta
    15001 acccttcaca tttacccaaa attagaaatt cgcccgcaat aaatctcctc gccactttca
    15061 acccctgaac tatgtttact tttagcaata aattaaaggg tttcatgctg aaagtgggga
    15121 tcaccggcgg aatagggaca ggaaaaagtt ttgtggccgg tatattcagg gtactgggta
    15181 tcccggtata tgaggctgac cggcgtgcta aagaaatcac cgaggaaagt cccggtttaa
    15241 aaaagcagat tatcgacttg cttggcaccc aggcctacac ccctgaagga aaatacaacc
    15301 gggttttcgt cagccgggcg atctttaccg atgcccggct gaaacaggcc ctggagtccc
    15361 tgattcatcc ggaagtccgc aaagatgggg aggcctggtt cagacgccag gaggaggaag
    15421 gtcatccgta ctgtttaaag gaagccgctt tattgtatga aagcggcagt gaccgggacc
    15481 ttgaccaaat catagtggtc gatgctccgc ttgaattgag gatccgccgc ctgatcaaaa
    15541 gggacgggct ttctgaaaat gagattatga acagggtgaa ccagcaatgg gcccaggaga
    15601 taaagaagga aaaagcggat tttatcatcc acaacgatgg caggcacccg gtcatcccgc
    15661 aagtgctggc catccatcac caattggtgc gcggcgcttc cgtatctttg ggctgaaacc
    15721 gtatatttta tgaattcaca ggattacatc aggctggaag atcggtacgg cgcccacaat
    15781 taccatcccc tgccggtggt tttgacccgg ggactgggag ccgaagtctg ggacattgag
    15841 ggtaaaaaat actatgattt cctctccgct tactctgcgg tcaaccaggg acattgtcat
    15901 cccaggatcg tcgccgcgct cacggaccag gcctcaaaac tgaccctgac ctccagggct
    15961 ttttacaacg atcaactggg tctgttcgaa cagaaggtca accagcgatt tggttacgac
    16021 cagtcgcttc caatgaacac cggcgcggaa gcggtggaaa cggcgataaa actttgcaga
    16081 aaatgggcct acgaggttaa ggggattgcg gccaaccgtg ctaagatcct gttttttgaa
    16141 tcgaacttcc acggccgcac gctcggcgcg atctccgctt ccacggagga aagcagcacc
    16201 ggtggttttg gcccttacct gcccggcctg gtcattttac cttacgggga tctggactcg
    16261 gcaagggaat atatcgaatc agaaccggac ctggcgggtt tgatcatcga acccatccag
    16321 ggggaagcgg gggtaatcct tccacccgag gggtacctga aatcgctttt tgacttatgt
    16381 aagaaaaaaa atatcctttt tttcgcggat gagatccaaa ccggcctggg acgcaccgga
    16441 aaatggcttt gctgcgagca tgaaggcatc cgccctgatg tggtccttct gggtaaagcg
    16501 atttcaggcg ggatgttgcc ggtgtccctg gtcctggccg acaaagccat catggaagtg
    16561 atcaggccgg ggcagcacgg ttcaacctat ggagggaatc ccctggctgc aagggttgcc
    16621 tccgtggccc tggatgtatt ggaggatgaa cacctgatcg agaatgcaga aaaactgggg
    16681 aagatgcttg gccttgccct ggccgatctt atggaagact gtccctggct ggtcggcacc
    16741 cgtggaaaag ggttgtttca tgccattgaa gtcgatgatg acccggaaag caaaaccgcc
    16801 tggaagatct gcctggacct ggcccggcag ggattgctgg ccaaaccgac caggggcaat
    16861 ataatccggc ttgcccctcc cctggtgatc agccaatccc agctcgaaga atgcattggc
    16921 atcctcaggc aggtatttat gtccagcgaa gccctcgtct gacctttatc atttcattaa
    16981 aggatgccgg atactaccgg gatccgtcgc tgcgttcaat ccaaaacctg cacattaacc
    17041 ctggggatca gcgatgatat tcgcgcttgc ggggagcagg taatcccggg ggatgggaga
    17101 atcttatttc gtggaagatc cccgatacat ataggttaat tttttcataa atttgtcctt
    17161 gaattacact taaatacgaa gtaatttcgt cgtagttttg atatttcatt taaccggatt
    17221 ttacatggcc aggtcaattc tattattcct ttttattatt cctgtcagtt ttcccctgac
    17281 cgcacagaaa acagccctat actcggaaat caatgcccat tataaagaag gccttcggct
    17341 tttccaggaa ggcgtgtatt ccggcgccaa aaaagaattc caacaggtcc tggaagatca
    17401 acgcctgagc ttcgaacccg aataccagac cctgcgaact tatgcggaat actattcggc
    17461 catatccagc ctcaggctca aactggatga agccgacctg atggtgacgg aattcattaa
    17521 aaagcaccgc cccgacccca ttgccgaaaa ggccaatctg gaagtggcca attattactt
    17581 tgaccaaaaa aattatgaaa gggccctcgc cttttacaat atcatggata ccaggggcct
    17641 gtccagtgaa tcgctcagtg aaataaagtt caaacaggga tactcccatt ttgccaagaa
    17701 agattttaag aatgcggccc tggccctcaa gacggtgagc ggcttgaaaa cggaatacta
    17761 tttccctgtc aattattacc tcggcatgtc gcaatatttc ctggcccaat acgatgaatc
    17821 gattaaaagc ctggatatcg catccaagtc caaaaaatac gccgatctga taccctatta
    17881 catcacctat atttattttt cacagggcaa gtatgatcag ttgctggcct atacaaagac
    17941 cttcctggat gggacacaga aagtggaccg gatcaacctg atccggcgca tggccggcca
    18001 cgcctatttt agcaaaaagg attattcctc ggccatccgt tacctggaag aatttgaaaa
    18061 aaccaatcaa agcctggaag aagcggacta ttacgccttg ggttacagcc attaccatct
    18121 cggccagtat cccaaagcca tcccctattt caggaccatc agcagccagc aaagccagtt
    18181 gggccagaat tcaaactatt acctggccga ttgttatctc cgttcggggg accggctttc
    18241 cgcccggacc gctttttaca atgtgagcaa agtccagtac caggaagata tccgggaaga
    18301 agcctccttt aattatggaa aattgtccgc tgaattggga tatgaccggg aaggtcttac
    18361 caccctgaac aactttccgg catcttcccg gtattacagc caggcgcagg acatcatggc
    18421 cgatattctg caaaggacaa aaaattatga gcaggccctg cgtaccatcg accagatgga
    18481 aaatccatca gcccggctga agcaagccta ccaggtcctg gcctacaacc gggggcttca
    18541 attgctccag gaagaccgct tggacgaggc ccgtccattt tttcagaaag ccattgatcc
    18601 ttccttcgat cggaatatcc aactttccgc catctactgg caggctgatc tgacccatcg
    18661 actgaaagat ttcgatcaga gcataaagtg ggcggaccga ttgctgagcc aggcgggaag
    18721 cggcagcaac ctcaaaaacc cggagttggt gcccatggcc agatacctgc agggatacaa
    18781 ctatctcaaa aaaggaaact ggctgacggc cggtgatcaa ttcaaacaat gcatcagcgt
    18841 tttaagtcaa aaaagtacgg gggcagaccc cgggctcctg ccggatgccc tcctgcgtgc
    18901 cgcggattgt tcgtttaaac ggaacaacta tgatgatgcc atcaatgggt atgcccaggt
    18961 gatccggaac aaatacaccg gaaatgacta tgcgcaatac cagaccgcca tcctccaggg
    19021 gctcaagggg aacaacaggg aaaaaataag attactcgat gatctgccca aaaaacaacc
    19081 gcaatccaaa tatgccgatg agtccctgta tgagctgggt gtcacctaca ccgaccaggg
    19141 ccagatggat gaggccaatg gcgttttgaa aaagctgatc cgtgattacc cgggttccaa
    19201 tatctataac cgggcgctga tccaactcgg gttggttaat tacaacctcg gccagaaaaa
    19261 tgaagcgctg acccattata aaaacgtgtt caagcacaat cccaccgcca gcgaaagcca
    19321 ggaagccctg gtcgcgatcc aggaaatcta tgtggacgac cttggacttg cagatgaata
    19381 catccgtttc atcgaatcgg tgccaggcta taacctgacc aattactccc gggattcgct
    19441 caattacagc gtggcccttc gttattttga agacggccag tatgaaaggg ccacgacggc
    19501 gttctcggac taccttcaga aatttccaaa cggggctcat tccctcgcgg ccttattccg
    19561 gcggggagaa tcttacaccc tgctcaaaaa gtacgatccc gcgctccagg attatgagcg
    19621 ggtcatccag cggggaagaa atgaatatta cgaccgcacc ctgttcaaag ccgccagcct
    19681 ggcctatcat catgcccagg atttcaataa agcgtttaaa tattacgcag aacttgccgg
    19741 ggcggagacc gcggacgaat cgaccgtgta tgaagcccag ctcggggcca tgcgttccgc
    19801 ttaccgaacc gggcagaccc aggatgtggt gcaattcggg gaaaaagtgg ccggtaatcc
    19861 gcaggcaaac gatgaacaaa aatccacggc ttccttttac ctgggcaaaa ttgcctacga
    19921 tcagaaaaat tttccaaaag ccaccggtca tctcaccacc gtcacccggc tgagcgacaa
    19981 tgtccagacg gcggaagcca ggtacttgct ggccaatatc ttatttcttc aaaaacagaa
    20041 cgcccaggct gaggaagcgg tcaaacaagc cattcagcaa aatgcggatc atcctttgtg
    20101 ggtcgcccac tgcctgatcc tgctttcgga cctttttgtg gaaaagaacg atctgtttaa
    20161 tgccaaagcc gccctggaag ctgtactgga aaattacaag gaagatccgt cgatcagccg
    20221 gatcacccag gagaaactcg atgcggtcaa taaaaaacaa cagaactcca gcaaactgaa
    20281 aacggacagc ctccgatcga attccttatt tgaaaaccat tgatcactgc cccatgaaaa
    20341 aaatattcct cccgatagcg gttatcgctc tgaacttcca tgccggcgcc caacaaaaac
    20401 tgccggtcga cgtgatccag gtcgtcaagg attttgacgc caggctcgac gaaaccgaaa
    20461 aaattaaaat cagtccagag ctcatagcct tggacaccac taaaaaaagc tatcagtatg
    20521 ctgtaaatcc aggggtcctg gatgtaaagt atgcggcccc gcagcttcgg ccggtacccc
    20581 tcaaaccggc ggacccccta cccgtctatc cattttacct caaagccggg tatggaatgc
    20641 ccaaccaggc ttatgggaaa ctttcctatg gatggaataa gaatgaccgg gcaaaaatcg
    20701 gatttgacct cagccaccac agcgccaaca acaataagaa aaacagggcg cagaaatttt
    20761 atgacaacga catcaaggca aatgccctct ttatcacgga agacggcatg gcgatccagg
    20821 gcgatgcatc tttcagcaaa gaccgttatc accacttcgg ccactacatc caaaatccca
    20881 ctaccgaaac cccggagcag atcctcaggc acgactacga cctcctgcaa atcggtgcag
    20941 gcatctttaa tcccacggtg agcaaaatcg ggatcaatta ctttgccggc atcaaatttg
    21001 ccagcctggc cgacaacctg ggcagtaagg aaaaaaatgc caatatccac ctcggcgcta
    21061 ccaaatggat caaggaccgc cattcctttg gcatcgagct gggatccgat tttaacaccc
    21121 tgaaagattc cgcgtcccag gaactgaata acttttacct cctgccccag gtcggcatcc
    21181 atggtaaatc attccagatt tcagccgggg tgcgggtcac ttcccacaaa gaagagatca
    21241 atcttttccc cgccgtcaac ctcagcctga gtattgcagg aaaccagctc atggctatcg
    21301 ccggggcaga cggaggatta aacaaaaaca gctaccgtac cctttccgac tataatccct
    21361 ttatttcgtc caggcccatt cttacaaaca acacgttcac cagctatttt gcaggaatca
    21421 agggttctgc taaaaccatt gaatacgatg ggcgcattgg ctaccggcag aacaacggcc
    21481 tgcccctctt tatattgaat accgccaact tcagccgatt tgacgtgatt taccgggata
    21541 ttgacctctt tcacattacg gcttcccttg ccttcaggcc aaccaaacac atcatcatca
    21601 gcagcgccct gaacaagaat ttttattccg ataatcccga acccaatgcc tggggacttc
    21661 catcgatcga attcaacggc tccgtccagt accaatccct gaatcaaaaa ttgttgttca
    21721 ctgcagaggc tttcgtcaac gacgggatcc cttatctcga tgatttggga aattccggga
    21781 aatcaaaagt gctgctggat atcagcttag gcgcggatta tcatctgact aaacacttcg
    21841 ggctctttgt ccaggccaac aacctggcca gcaaccaatg gagaagatgg taccagtacc
    21901 ctacttttgg aatcaatgtg gtgggaggaa tcactttaaa attctgatcg tggggaaaat
    21961 acaaggttcc caaccaccaa ccaattctca ccctttcaca tacatcactt ctttcaccgc
    22021 gcggatcacc ttcgccggat tgggcaacca ggcgtccacc aaagtcggag cgtagggaag
    22081 ggaggtgtcg gctgaattga ccctggtcac cggcgcatcc aggtaatcaa aggcgtattt
    22141 ttgcatttca taagccactt cggcactgac gccggaaaaa ggccaggctt catccaccac
    22201 taccatgcgg ttggtttttt tcacggattc aaccagggta tggaaatcca gtggacggat
    22261 cgtccgcaga tcgatcacct cagcctctat tccttcctta cttagttcct ccgccgcttt
    22321 tttggccact tcggccattt tattataaga gacgatggtc acgtccttgc ccggtctgcg
    22381 gatcgcggct ttaccgatgg gcaccaggta ttccccttca ggaacatccc ctttgagacc
    22441 gtaaaggatc tcggattcca tcatgcacac cgggtcctgg tcccggatgg ctgatttaag
    22501 caggcccttg gcatccgccg ggtccacgca ggaaatcacc ttgagtccgg gtacgttggc
    22561 catccaggat tcaaaggtct gggaatgttg ctgggccagt tggccggcac tcccgctggg
    22621 tcccctgaaa acaatgggac aggaaaattg tcctcccgac tggctcaggg tcttcgctgc
    22681 attgtttact atctgatcaa aagcaaggat ggcaaaattc caggtcatga actcgatgat
    22741 cggccggagc ccgttcatgg cggctcctac cccgatgccc gcgaatccaa gctcggagat
    22801 gggcgtatcg atcacccgct ggggcccgaa ttcatcaagc atccccttgc tcactttgta
    22861 ggcgccgttg taggcggcca cctcctcacc catcaggaaa accagaggat ccctgcgcat
    22921 ttcctcaatc atggcttccc ggagggaatc cctcaacgtg atcattcggc tcatcttctg
    22981 aattttggcg caaatttagt caaattgaag acaccatgcc caggcgggtg ccgcgattta
    23041 taaaaccggg tagaacgctt ttctttattt tacacgcctg cttatgcatg aagaggaaat
    23101 caaagacctg ctgaaaaaag catttcgcct ggaaggcagg ctgaccacgc tctattctga
    23161 actggatgcg gtgtacgttc tcgacacggc ggagggcggg aagcttgtcg tcaaacaatc
    23221 ccatcccgga agggatcctt cgatcctgga gatggaggac cgcgctatac tttatcttaa
    23281 tcagcaaggt tatgatttcc agcttcagga agtatttatg gcgccggacg gtgatcgtat
    23341 ggtacgatac caggatcacc attttcgggt atttaaatgg atagcccttc ccctctgggc
    23401 ggaatgcaag ccggtgacgg caaacacccg gaaatcctta ggtaaaatga tgggccgcct
    23461 ctcctcctgc ctggccggtt ttgaagaccc cgccgctcac cgctttatca aatgggatcc
    23521 atcccgcctg ctctggatcc gggattttgt gcacctccat cccgacccct ggaatcagaa
    23581 gtttatcgcc tggttgaacc actatgaaaa aaacgtattg cccctcctgg attcctgtcc
    23641 caaacaaatc aattacaacg atgccaacga ttacaatgta ttgtgccggt ggaatgaaga
    23701 aattcagtcc tatgaaaccc atgccctgct cgatttcctg gatatggtat atacgcaccg
    23761 gatcaatgaa ctggccatcg cctgcactta tgccatcctg aaacttccag accctctccg
    23821 cgccgcctgc gaagtggttg gcgctttcca caaggcctgt ccgctggagg aggcagaatt
    23881 ggaggttctt tatgctcaga tcaccgcacg attgatgatc agcctgacgg tttcagccat
    23941 aaacaggcaa gaccacccgg aaaaccaata tctgcaggtt actgaccggg atgcctggtc
    24001 attgttggaa gcttggctca acgtgcatcc acggtttgcc ggctattcct tcagagcggc
    24061 atgtgaaaaa aagccggtcc cgggaatgga ccggtggatt caaaaaatcg gagcttcccg
    24121 ctttcatccg gtactcgatc attttatgga tcatgatcct tatctctttg atttcggggt
    24181 gggaagtacc gagctgggta atttttcagt cttcacggac gacctgcggt tggataaaca
    24241 ggtacgccgt atcctggaag accatggaac ctccaggggc ataggcaggt ataaagaaat
    24301 ccgtcctttt tatacttcgg aagcctttcg gagagaaggc aatgagggtc cggactggcg
    24361 aaccgtgcac ctgggactgg acctttttgc tcccgcaggc actgtagtgc gtgcgccctg
    24421 ttcaggacgg gtccatagca tcagaaacaa cggcgggcac cgggattacg gcccgaccct
    24481 gatcctcgag cactatctgg aggaatttgt tttttacacc ttatatggcc acctgggcct
    24541 ggagttattc agcggactgg acaggggaca agccatacct gccggatttg agatcggaaa
    24601 gattggcaac attcacgaaa acgggggatg gcccccgcac ctgcattttc aaattatcct
    24661 tgacctgctg gatcacgagg gcgattaccc aggggtggcc agccctgggg aattgatcgt
    24721 ttgggaagcg ctgagccctg atcccgcact gattactggt attttttccc tgcgtgagga
    24781 ggaaccctct cttacggaaa tgctcaggct tcgcaaggag agattgggtc ccaacctgtc
    24841 ggtttcttac cgggaaccgc tctggatccg cagggggatg atgcaatatc tcctggacca
    24901 gcatggcaga agatacctgg atacggtcaa caacgttgcc cattgcgggc atgaaaatcc
    24961 caggattgtg gataaaggaa ggaaagccat ggccgtattg aataccaata cccgatacct
    25021 ccatgaaaac atcttaggcc tggcagagca gctcaccgca gcactcgcac cctccctccg
    25081 ggtctgttat tttaccaact cgggcaccga agccaatgag ttggccattc gaatggcaaa
    25141 acaatacgcc ggacaggagg acatcctggc catgcaatgg gcctaccatg gaaacagcag
    25201 cctcatgata gacctgagct cgtacaaatt taaccgcaag ggcgggagag gaaagccccc
    25261 tcatacccac ctcatcccga tgcccgatcc tttccgcgga aaatatgccg gtcggatgga
    25321 ccaggcggag gcttatttta aagaagttga aaatatcatc aacggtttga aagagcaagg
    25381 tcatggcccg gcggccttgt ttgccgaatc catattgagt tgcggtggcc agatcgtcta
    25441 tccggatggt ttcctggacc gggctgtagc tatggtcagg gcagccggcg gcctttatat
    25501 tgccgatgag gtccaaaccg gcctgtggcg caccgggcgt ttctgcgctt atgaattcta
    25561 tggggtcgtt ccggacattg tgacctttgg caaaccgctt ggcaacgggc atcctgtcgg
    25621 ggccgtggta tgcacccgtc cggtggccga agcctttcac aacgggatgg agtacttcaa
    25681 cacttttggg ggaaacccgg tcagttgtgc catagccggc gaagtcctcc gggaattgat
    25741 ggatcaaaaa agcatggacc gggccaaaga aatcggggat tccctaaagg cagggctgaa
    25801 aaatattcaa acacgctatc ccctgattgc agacatccgg ggtcacgggt ttttccttgg
    25861 aattgaattg atgaaaaatg gacttcctgc cagcgccggg gcttcttacc tggccgggag
    25921 gatgaggcaa tacggcatcc tgatgagcac tgacgggccg gatgacaatg tgcttaaatt
    25981 caagccccca atgtgtttca ccggggatga tgcgacccag ttgctggacc gtttggaaaa
    26041 aatcctggcg gaagatcccc tgcggatcta agccaggcag tacggggcta ggcggtgttg
    26101 tcccgcttgt tcaggggaat gaccagcttc agggcggacc agttctcatc gactgaagcc
    26161 actttaatat ccaccaaatt attcctgagg gcggcgtccc gtatgaggtc ctctgtaagt
    26221 tcggtaggct gttttgagga ctttttaaac caggagatcc agatcatgcc ggtactcttc
    26281 agcctggttt tgagcccggg caaccgggct tccaggtcgt cccgtttgtt gataaaaccc
    26341 cataccaggt cgtacttctt gcggccaatg gctttaacat cgaccgggat attgccattc
    26401 cggaatagaa agagtataaa atccggtgcc tggactaaaa ggatttgctg ccccggctgg
    26461 atcccgattt tacccagcaa gggggtctca taggttttgt gaccggacct gctgtatatt
    26521 tttttcatgc attttgtcta aaaaagaata gaagggcgag gtgccccagg attcctgcca
    26581 gggctttatc ctctcggaac cttcaaggct tccgaggtat gccttagacc tttcgatcct
    26641 atccaggatt tctgccctgc ggagtgcaac attgccatgt ccgggaacca tgacttcgat
    26701 gggaaagtct tccagaatac gggatgcggt ttccagggtt tgtgcataag cccccaggtc
    26761 cgtcacgaaa ggaaattcaa tatcggataa atagtctccg gcgatccata agcccatgga
    26821 ggggatcagg gtaaatagcc catccgcggt atggcccggc gccgggaaaa aatgaacctc
    26881 cacatcgctt agcctttgtg tgcttggacc cttgtcctca accttgatat ccagctttgg
    26941 aaacctgggc gggaagtctc tccgaacata atactcatga tctacggctt cgatcaaacc
    27001 caatgcttcg gccttatccc ttcgttctgc catcccaaag gaaccgaccc gcaaaggtcc
    27061 ggtaaaggcg gaatccccgg cgatatggtc ataatcccca tgggtatata ggaggtagtg
    27121 gtcaacaatc ccggacggat ccatcgctgc ccggatcctt tcaatttccg ccgcggagta
    27181 ggcagggtcc cagcaaagga cggtcttgtt ttcatgataa accacggagt tgagttgcca
    27241 aaaacaactt tgaaaaatcc ggtactgacc catgtttttg tttccctttc cggctggtaa
    27301 aaataaaatc caaccgctct aaaaataaac tttaaaaccc gtgaaagtca tattactaaa
    27361 aaaatttata atgatttttt ttaatgaatt attcatatta aataatagtg caatatgtaa
    27421 aaaactgttt aacatactat aataggtaat tgttttaact catctgtttt cggggcttat
    27481 tttcctgaaa tccagtcttt taaaccccgc gttttggtat ggttttggaa cagctagggc
    27541 aagcaaatcg actttatgag tttaaatttt ttaaatctat tgattcaatc aaacactttc
    27601 tagaatgaaa aagacaatta ccttcctatg tctgaaacga tgctggctgc cggtggtcct
    27661 ggcattgttc tactcattga gcatgagtgc ccaggcggat acctgcaacc tggtgtgcaa
    27721 cgatcatgtc aatgcctcca tgccggctga tggatgtctt aaaacctttg tgcccgatga
    27781 tttccttgaa aatccgggtc tcacctgcac cacttatgat gtggagttga gctatcctta
    27841 tggtacggcc aaattaccag gtggtgacca ggtcaaccga agccatatcg gttatacgtt
    27901 catctaccgg gttattgaga cggtgagcag gaacagctgc tggggatatg tcaccattga
    27961 agacaaggcg gccccacaac cggtgtgcaa gaatgtaagg gtctcctgtt tccaggtagc
    28021 acgattgtca gaaatcgtgg gtgaaatcat agacaattgc ggccaggccg gctcttctgc
    28081 cattgagagc atgcagtggt cggattgggg ttgcgacagc gtctcgggcc ttggccgggt
    28141 gatccggacc ataaggacct gggatgattg gggcaattcc gccacttgtt cagataccct
    28201 caccatagga agggattccc tgtccaacgt cacctgcccg gatcccatcg ttttgggctg
    28261 ccgggtcaac tgtcagggca aagggatcac ccttatatcc aaagacaaaa acagtcccaa
    28321 ctacccatcc ccggattttt tgcttgatct tcaaaaaaga gatacggctg gcggcggacg
    28381 caaatgtgta ccggcaggac tcaaagtggt tccattcatc gacgatagcg tgctggtcat
    28441 gcaaggatca gtatgcatac tcgttgattc ttccgtagcc atgtatcctg ctccgggtgg
    28501 tttctgcaag accagcctga cctggacaga ccagatcatc ccgatttgcg gtaccggttt
    28561 taaaatcaga agggagtggc tgatcgtgga ttggtgtaat cgtacggaca cgacctgtgt
    28621 tcaatacatc aaagtagagg attccgaaga ccccatcgtc aaatccaaca gtaagtttta
    28681 ttacaaagca aataccagtc ctcataattg ttatgccaac atcaacctgg atgctttgat
    28741 cgtggacgat tgcgatgtca atgcaaaaca aagttatatt gcccaataca ccctggagag
    28801 ccatccggct aagatcaacg tgatcaacgg tactttgccc ggtaaaatca gccttccggc
    28861 catcccatca gactacaaag gcaaagcttt tcaccgcctt gaagtatcca tcgtggatgc
    28921 ctgtctgaac cgcacggaca ccgcgatcta tgtaaaggtc agcgacgtga cccccccgaa
    28981 tccggtatgc gatgaataca cccgtacgac ggtggatccg agcacttgtt gggcaagggt
    29041 ttatgccgcc gatctcgaca acggaagcag ggacaactgc tgcaatgtat tgcattttgc
    29101 cgtggcccat atggatacca tcgaagctgc ccggaatgcg tggaaatcgt actggacggc
    29161 caattgcaat tcaacctatt ggaaatataa agaccgctgg gacgattggc tcgaagcctg
    29221 gatcaatgtg ttcattttta aagactatat cgacctcagc gaatgcggtg aaaaccaggt
    29281 ggtcctcagg gtgtatgaag cctgcggggt acccctttac gatccccatg ttttcccctg
    29341 cagcaaacac gattggttta cgtacaattc ctttaagctt tccaggttgt ggcataatta
    29401 caagtttttc tacttgaatg acaaaaccag tccgaattat aaaaactgta caagcagttt
    29461 tggaccgttc tgtaaaccga cccattggga tccgccgtct gtggattaca gtggcagata
    29521 tcccggttcc gagacctatg tgcggagttg cgagtttgaa tttggagatg aagaatccct
    29581 gccttctgcc accaataatt tcggaggcct ggaacctgga aacacctgtt ctgccaggtt
    29641 gtacaacgat tgtatggtca tcatctacgt ggatgataaa accccgccgg tctgcgaaga
    29701 acctgccgac ctttactggt actgcgacaa cgtagccgac gcgcgggaga tcaagggcaa
    29761 aaaatacttt atccgcggcc accgttacaa ccggctcgaa tatgcccatg ccgtatgcga
    29821 catcgattac cacaacgaca atattcttgc cagcggcaac ggcaaatacg attacgaccc
    29881 caccctgcat gccgatgacg gttcctgcag gtacgattac accccttata acgagattga
    29941 atgtgaagaa gaatttgacg gcgaccttac ggatgcagtc gatgccacag gcaaagcctt
    30001 tggctggtac ggatgcaata tctatggccc ggcgcaccag gatgagcatg gctattacaa
    30061 ggaaatctgt gaagatgacc atgacaactg ggccccgatc tattgtcata cctggctctg
    30121 cctggaccgc aaagactccg gtaccaaagt ggatcccaag accgctttct ggactcctgt
    30181 tctcaggagc ggcggacgcg gcgcagatgt ggatgaagga cagttcatca tctgggacaa
    30241 ttgctggatc ggtactatta cctcggtgga tgaatcctac gtggacaact gtggtaacgg
    30301 ctggttaaaa cggacctgga cgactaccga caaatgcggc agcccggtcg tttgcgacca
    30361 gaaaatcatc accaagcacc gttctgattt tgaagtggaa ttccccgcgg atctcaccgt
    30421 gagttgcgat gtcgaaggca gccttagccc ggacggcacc ggaaggccta tcatcatgga
    30481 cgacgaatgc gaactggttg gcgtcaatta tgaagacgta cgttatgaca tcgttcccga
    30541 cgcctgctat aaaatcgttc ggacctggaa actgatcgac tggtgtaaat acgatcccaa
    30601 cgcgcactac cgcaagccgg aagtgatcgt ggatgaccgg aaagtagccc accccaccga
    30661 tcgttattgt gtgtacagac atctgaagga tgaaggggac ggatacatca cctacaccca
    30721 gatcatcaag gtcatcgaca ctgtcgcacc ggtgatcacc tgcaccgaca cgaccatctg
    30781 cctgtatacc ggtaccgatg ccaattgtac ggcccccaac ctttccgtgc ctttcatcgc
    30841 gaccgacaat tgtacagcgt ccgataagat ctccttccgc tgggaactgg atctcaatgc
    30901 ctctgcaagc gacgttgcca atcacaaata caacaaggcg agcatcgatg acaagagtta
    30961 tacgccggtg acggccttct cttccaatac catcaaagac ggaacccacc tggtacacgt
    31021 catcgcttcc gacaattgcg gaaatgtgga tacctgcacc tttgtcctga ctgtaaaaga
    31081 ctgcaaggaa cccactcctt attgccacaa cgggatagcc acggtcatca tgccctcgac
    31141 cggtgaaatc accatctggg ccagcgatct cgatgccgga agttatgaca actgtacacc
    31201 aaaatccaac ctggtgttca gtttcggtcc cgacaagagt gtgaccagca aggtcttcac
    31261 ttgtgccgat attccgaacg ggacttcaaa caccattgag gtggatatct acgtatggga
    31321 tgcttacgat aacaaggatt attgccgcac ctacctcctg cttcaggacg ggattggcaa
    31381 caaatgcacc gataatcttg aaaacgctgg atctatagcc ggcaaaatct ctacggaaaa
    31441 tgacgatgcg gtggaaaagg tgaaaatgaa tgtgaaagcg cctcaaaaca tgccggccta
    31501 cctcaccgat gtgaaaggac agtattcctt tggcggcctg cccatgaaag cggattacag
    31561 cattatcccc gcccgcaacg acgacccgat gaatggtgta agcaccatcg acctggtgct
    31621 gatccaaaag cacatcttag gttcaagccc gctcaattcc ccgtataaga tcattgccgc
    31681 cgatgtcgac aaaaacaccg acatcacggt actcgacctg gtggaattga gaaaactgat
    31741 cctggccgtt tacgatgaat tgcccaataa tcaaagctgg agattcgtac cgaaaaccta
    31801 ccagtttgaa gccggtaaaa acccattgaa gacctccttc ccggaacaaa ttgatatcag
    31861 caaactgagt caaaatgaac tgaacaggga ctttgtgggc atcaagatcg gcgacgtcaa
    31921 caccaccgcc accccgcaca gcttgctggg tgccgaagcc cgggaggcaa gcgctaccct
    31981 gcaattcaga acggaggacc gccagctcaa agccggtgaa accgcaacga ttgatttccg
    32041 tgcggacaac ttctcaggta ttgaaggtta tcagttctcc ctcgcgctcc atggattgac
    32101 cctcgtggat atccaaaagg gcagcctgga agtggatgcc agcaactttg gtttgacaaa
    32161 actgaaccag ggttacctca cgaccagctg gagtaaggaa aaaggcgccc gcctgaaccc
    32221 gaacgacgtt gcattcagcc ttaaagtgaa agcaacgaaa gacgtgaccc tcagcgagag
    32281 cctgaaaatc aattccagat tcacccgtgc ggaagcctac gccgaagacg gtaataatac
    32341 caaatatctt ggggtaagcc tggagattgg aaaaagctca agaccttcca attacgtcct
    32401 ctatcaaaac acgcccaatc cattcaaagc ccagaccctg atcggatttg agctggccaa
    32461 aaacgaagcc gtaaccctta gcgtcacgga tgtcaccggc aagctcatta aatcctacca
    32521 gattgacggg gtgaaagggg ccaaccaact ggtggtcaac aggaatgaat ttggaagcgc
    32581 cggagtcctg tactacagca taaaaacgaa atcattcagc gataccaaaa agatgttgtt
    32641 ggtggaataa ttccagtagc ctatttgaaa aggatctcac cattgatctt tttttagaaa
    32701 tgttgtcttg attaaaggac ccggccggcg gccgggtcct ttttttttaa accctttgat
    32761 tttaacgatt gagcccctcc tgccatttaa acatattacg gccccttatc tatgtcattc
    32821 gctgtgattt aaacccttag atccttgcat taaaactagc ttgtataagt taaaggaata
    32881 ttccaaatag ctgggtttga agcatgattt ttgcttaagg ttattttaaa tttcggactt
    32941 tataacaaat tcataatcac cgtgaaaagc ctatttagat tctgtttaat tgtaagtgtt
    33001 tgtgccggtt tcttcacccc ccctgcccac gcccagtgca atttgatttg caacgatcat
    33061 atccacgctt cccttcccgg aaccggttgt aacaggacgt tcgacgcaga cgatttcctg
    33121 gaaaatccag acccttcctg tgcctatacc gtcaagctga cctacccttt cgggacggcc
    33181 aagctgaacg gcacagaggt caaccgttcc cacattggtt acaccatgat ctacagcgta
    33241 tcggctcctg gaaacagttg ctggggtttt ctcacggtgg aagacaaggc cccgccgcag
    33301 cccttttgcc aggatgcggt gatctcctgt ttccagctcg cccgctacaa tgaactggtg
    33361 gaaaagatcg tggacaattg cgcggaggat ggcaaatcca tcatcgaatc cctgcggtgg
    33421 gaggaattag gttgtacttc accggtaggg attggccatg tgatccgcaa gatccggtct
    33481 ttcgatacct ggggaaactc cgggacctgc attgacacgc tcaccatccg gcgggattct
    33541 tttgaattga tcaagtgccc ggacatggtg accctgaatt gcctggtccg gtgcaaaaaa
    33601 ccaaacaatg acggggcaaa caacgaccct aagaattttg atctcatcta tttctcctcc
    33661 aattcttcca gcccctacta cccgacaccc gaaaaactcc tggaccttca aaaaagagat
    33721 actttcaaca gcggcgaccg caagtgcata tcgcccctgc tcaaggtagt gccgtttatc
    33781 aaagacagtg tattcaacag cgaaggggaa ctcatcgaca cctgcgtaaa catgtatcct
    33841 tcgggcgcag tcctttgtaa aatagtgttc tattacacgg acttggtatt ccccatctgc
    33901 ggaacgggtt tcaaaatccg gagggaatgg agatttacgg actggtgtac cggagaagac
    33961 acggtctgca ttcaatacat caacattctg gacaaacagg ctccggtggt caaaaacagc
    34021 tttagcattc ctgtccagat caaggatacc ctctatgact ttgtttttcc gggttatcgc
    34081 agggatctgg tcacgaatac ccacgactgc aaccgcacct tctgcctcga cagcctcaca
    34141 acggaggatt gcagcaaggt gacccaaacc tatgaattta cttatacaga tcccaatggc
    34201 gggaacagca tcgtccatag gaccggagga ccgaaggatt gtttcaccct ccctggcgcc
    34261 ccgaacaatt accttttttc aggaattgtg atgggcaccc cccttgaaca ttataaggaa
    34321 atctcctggc ccagaaactg ctatcccctt tttattcaga cccgggacga atgttttaac
    34381 accactgcag gtgtttcaaa actgattaac cgtaaaaccg gtcaattggt ggcggaaaat
    34441 ccgctgaact ttgccggaat tatcctggtt tgcatttccg atgagacccc tcccaacccg
    34501 gtctgtgatg aatacaccca aaccaccctt gaccctgaac agtgctggag caggatatat
    34561 gccaaagacc tcgataatgg aagcagggac aattgttgcg agctcctcca tttcgctgtg
    34621 gcccacatgg attccatcac ccattaccgg gaacgatatg cccagcagat caaagattcc
    34681 tgcggcactg cggaatacga caaacaccgg aaaaagtatg ctgagatcat cgagaactgg
    34741 atcaactgtt atgtattcaa cgattacgtc gacctcacag actgcaatga aaatgaactg
    34801 gtgctccggg tctacgaagc ctgcggggtt cccaggtacg atgaacacat ttattcctgc
    34861 agcccgcatt cctggttctg ctacaatacc tacccctctt atatgttgtg gcacaattat
    34921 attctggata ccgatccggc caggaagtgc cttacgcccc tgccctggct ctgcctggat
    34981 aaacaatgga aactcatttc acacctgatc tacaagtccg gaaaggatta ttggaggccc
    35041 aaatatgccg gtgcaaccca attgattccc gaccaggagc tgctttatgg tccggcggtt
    35101 tgttatccgg agttttacaa cccggctcag aaggcggtat ccgaacccgc ctatgccccg
    35161 ggcaacacct gcagcaaaag gctgtacagt gattgtatgg tcacggtgtt tgtggacgat
    35221 aaaaccccac cggtggctga aaaaccggat gacctgttcc tttattgcga caacagcgcc
    35281 cgtgaacaaa aatacagtaa tgaatatgcc aaatgcgacg acgattcctg gacgcccgac
    35341 aacggcaagg atttgcgttg tcaggatcag aaatacaaac cttacaacgc catcgaatcc
    35401 atagttgaaa acgacagtga tcagggggat accatcgatc ctacaaaaaa agcctatggt
    35461 tggtacggtt gcaacatcta tggggcctcc caccccgatg agcacggaga aattgccccc
    35521 tgcataccgg aagcaaacac ctggttcccg atatattgcc gcagttggct cctgaaagac
    35581 agttccgaca ccggtgcccg ggttgatccc ctgacccaat tcagccaacc ggtcctgaac
    35641 gatggcaacc cgggaaatgc gagccccggc gcagggaaat tctggatctg ggacaattgc
    35701 tggatgaacg attccagcct cacctatacc gacgaatcct atttcgataa gtgcaacaat
    35761 ggatgggtaa aacgtacctg gacagcccgg gataaatgcg gcaatacggt ctccgtggat
    35821 caaaaaatcg tgaccaaaca ccggtctgat tttgaagtcg tattcccagc cgataaaatc
    35881 acggtctgcg atcttggtga agatgtatcc ccggacgcca tcgggcggcc catgatcatg
    35941 gacgatgaat gtgaactggt tggggtcaac tttgaagacc agcgttttga tatcgtaccc
    36001 gacgcctgtt acaaaatcat ccggacatgg aaaatcatcg actggtgcaa atttgatccc
    36061 agccagcata gccgcgacca ggacatcatc gttgatgacc ggctggttgc cgatgaggtc
    36121 aaaagaccct gcgtgtaccg caacctgaaa gacaacgggg atgggttcat gacctatacc
    36181 cagatcatcc tgatccggga taccatccca cccctggcta cctgccggga taccacggtt
    36241 tgtatttacg atgaaaattg tgctttgcct tcgatttata tccccttcac ggctaccgac
    36301 aattgcacag cgcccagcct gatcagttat cgatgggaaa ttgatgaaaa cccctcccct
    36361 gccgacacgg ttgcaaagag atataacaaa tccagcattg acaaaacgag ttccggcccg
    36421 cagcaagcgc tttccattgt tcagccgcag gggatttccc tggtccatgt gattgccgag
    36481 gacaactgcg ggaatgaaga cacctgcacg tttatcctcc gggtgcgcga ttgtaaaaaa
    36541 cccactcctt attgttatca cggcgtagcc accgtgatca tgcccactac cgg
//
LOCUS       DAOQXT010000041        35186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1423_length_35186_cov_6.465714, whole genome shotgun sequence.
ACCESSION   DAOQXT010000041 DAOQXT010000000
VERSION     DAOQXT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 35186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35186
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1423_length_35186_cov_6.465714"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PKM27_09040"
     CDS             <1..764
                     /locus_tag="PKM27_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNR07447.1"
                     /translation="MVKEFKSDSLRIKIFENAEALGRAASTAVAQKLEDTIRLKGRAN
                     LILATGASQFAFLEYLQKEMVDWEKITVFHLDEYLGIPESHPASFRKYLKERILDQVR
                     PGRVYYLEGDAADTEAEAARYESLLKDHPIDLACIGIGENGHIAFNDPPVADFNDPRL
                     VKKVALDQACRLQQLGEGWFQSLDEVPTHALSLTIPAIMSCTSISCVVPDERKAKAVF
                     GSVHGEISTACPASILRRHPDTVLYLDPFSAKLLS"
     gene            complement(833..2599)
                     /gene="aspS"
                     /locus_tag="PKM27_09045"
     CDS             complement(833..2599)
                     /gene="aspS"
                     /locus_tag="PKM27_09045"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNR07448.1"
                     /translation="MYRTHTCGELRIENAGQTVTLSGWVDASRDFGGMTFMDLRDRYG
                     ITQIVFNMDSDAALCEEARKLGREYVIRVTGEVRERSAKNLKRLTGEIEILAKNLEVL
                     NAAKVPPFTITEDTDGGDDLRMKYRYLDIRRHSVQSKIIFRSVLGLETRKYLSGHGFI
                     EIETPFLIKSTPEGARDFVVPSRLNPGQFYALPQSPQTFKQILMVAGMDKYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVHREDILQTFEGLIHHLFLSMLEVDLGAFPRMSYDE
                     AMQRYGVDKPDLRFGMPIHELNEKAKGKGFSIFDQAAYIGALVLSGEADHYSNKEIKE
                     LTEWVQRPQIGAKGLIYVKCLEDGSFKSSVDKFYGAEDLRAWADFTGAKPGDILFILS
                     GEQDKTPKQLGELRLELGRKHFLIQAGDFKPLWVLDFPLLEWDEEAGRFFAMHHPFTS
                     PKTGDIEKMASDDRAVLAGIRADAYDLVINGTEIGGGSIRIHDRGLQSKNFSLLGFTP
                     EQAQDQFGFLLNAFEFGAPPHGGIAFGFDRLCAVMQGQTSIRDYIAFPKNNMGRDMMI
                     DAPSAIDDKQLKELGVNVVKSE"
     gene            complement(2658..3869)
                     /locus_tag="PKM27_09050"
     CDS             complement(2658..3869)
                     /locus_tag="PKM27_09050"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNR07449.1"
                     /translation="MGRYKMDAWAPEKELLCGIDRMETSSPPDPSLVYGLVTNDSAVN
                     KSGMPVKQALLKAGYRITRLFSPEHGLQARGVDGAAQPDRTDPGSGIPVTSLYGDRLE
                     PPPETRSGLDAILFDLPNAGVRFYTYLWTMSYMMEWCDKEGLPLIILDRPNPLGADLS
                     RAEGPMITPGYYSFIGRWNIPIRFSLTMGELALLLKYEMGLEKLSLQIIKTEGWNRMD
                     TILDWRYPFKPPSPALLSPTSYLTYPCLCFLEATNVSVDRGGPGSFEVAHAPWIRAEE
                     LTDAFNLYTPPGMEALPYRCVEPQGQYPGLSGEGLRLRVTDPAVFRPVKTGMILLALL
                     RTSYPDHFSWVPYPTLVNPSGARHFELLSGGEGLKSWLENEPLRHLDELDDLLAVPSW
                     AERVEAFLLYR"
     gene            3884..5311
                     /locus_tag="PKM27_09055"
     CDS             3884..5311
                     /locus_tag="PKM27_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR07450.1"
                     /translation="MKWTFTSLLFVLSASAFGQLSKTELYKNIDRRDAEYSGIAQNIW
                     DYAEVGFQEFKSSNLLKSLLKKEGFSIQDSVAGMPTAFIASFGTGKPVIALLGEYDAL
                     PGLSQDRVPYLKILDGKPAGHACGHHLFGTASAAAAIEVKNWLKASGTVGTIRFYGSP
                     AEEGGSGKVYLVRENQFRDVDVVLHWHPADANNASASPSLANKTAKFRFYGVASHAAM
                     SPERGRSALDGVEAMNAMVNLMREHIPSDARIHYVITRGGEAPNVVPAFAEVYYYVRH
                     PRVETVSSIWERIEKAALGAAMGTETKAEWEIISGVYNLLPNAAISGLMDKNLRISGG
                     YSYIPEEKDFAVKIQSTFRGTLKDLSGTSAIAPFAVSDVPWPASTDVGDVSWVVPTAG
                     LSTATWVPGTTAHSWQAVAAGGTSIGIKGMLVAAKTLAGTAVDLFNESGLIEEAWKEL
                     KMRRGEPFEYKPMIGDRKPALDYRN"
     gene            complement(5366..6793)
                     /locus_tag="PKM27_09060"
     CDS             complement(5366..6793)
                     /locus_tag="PKM27_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HNR07451.1"
                     /translation="MSAFQGSVPESPRPVFHFTAPQFWINDPNGLIYYKGEYHLFYQH
                     NPFGNEWGHMTWGHAKSKDLVQWTHLPVAIPEQKDHMIFSGCVVHDQKNSSSLGSASR
                     GPLVALYTAHREGHQAQHIAYSTDDGLHWNLFKNNPVLDLKKQDFRDPNVFWHEPTAR
                     WIMAVVLPKEFKVQFYASKNLIKWDLLSDFGGHPSFKEIWECPALFSLPVKNQKDKTK
                     WVLLISTNREFSSMRYFTGDFDGTSFRCDHALDQVNRVDAGKDFYAAIPFYQSPDQKK
                     ILLGWMNNWAYANQNPTESWKGMMTTARELGIQRESDGSYRLLQAPVESLQAIRKKER
                     EYHADQGTKFDTWEIQTSPAMEWQISLDSHKGVVLDFDFGKKAKLLVTWDATRRQLCL
                     DRTSKSLAFHQAYPSIECTGVSPGHNLTLQILIDRYSLEIFADQGKAALTSVFFAPGA
                     LQKFRINGKQFQAKATLWDLDPLAP"
     gene            7005..9602
                     /locus_tag="PKM27_09065"
     CDS             7005..9602
                     /locus_tag="PKM27_09065"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNR07452.1"
                     /translation="MSKGISFILLSVLFLACSTSRKSRVAEIKLSGPTATPVLPQPAR
                     DTLKPKADTASVESGRRAPAAKNGIRPYVEIITSKSVSRPGMFQVHQVEDRYYFEIPD
                     SMMGRPMLAVTRFVRTPTSAGYGGELLNRQMIRFEKGTEKKVFVRATALVNTGSDTSA
                     PIYKAVLNSNLDPIAAAFDIKAIRKDTSVLIDVTDFFSGDVQIVGFSNPIKTRYRMGG
                     LQSDRSFIQSIKPYPINIEVKTVKTYAISPPPNSSGPGSPQTVTLPGATASGFASMEL
                     NTSLILLPREPMRKRLFDPRIGYFSTGYTVYDEEGQRTEDPSFAVRWRLEPKNESDAE
                     RQRRGELIEPAKPIVYYIDPATPKKWRKYLIQGVNDWQKAFEQAGWKNAIRGEEFPEH
                     DSTMSLEDARFSAIRYFASPVENAYGPNINDPRSGEIIESHIGWYHNVMKLLNKWYRT
                     QTAAVDPKARPNEFEDELMGQLIRFVASHEVGHTIGLRHNFGASSATPVEKLRDKKYI
                     YENGHTSSIMDYARFNYVAQPEDSVTDLFPRIGDYDKWAVEWGYKPIYGTMDEKEDQQ
                     ILNKWFLTKAYPNRRLWFLTEINPYDPRAQNEDLGDNAMLASEYGIKNLKRIVPQLPD
                     WTREEGQDFKVLKEAYESAVDQFRRYLGHVTKYVGGIYETPKTFEQNSPVYEPTPAPL
                     QRDAVRFLSRHIFNTPEWLIDPSILNRIQPGSGLENIRAIQEPVIQGLFNEDRLQRMI
                     ETAVRTPSAYRVTDLFRDLQSAIWSDLNGSGEIDVFRRNLQKIYVERLISMLTTPVAP
                     SNAVTVFGTTLPARTDPKKNDVLSLVRGHLKALRPVIMQAAGRRGSSVSGMHLDDLAE
                     RIRKTLEPR"
     gene            9900..10469
                     /locus_tag="PKM27_09070"
     CDS             9900..10469
                     /locus_tag="PKM27_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07453.1"
                     /translation="MSAKRLILVLCLISSLRGTGQIQYTRDFTARLRSAGLELSLPDS
                     TWYKVHLPCEDVYNRYDLCLVSETDSADLKYLILDALQTNKIFSPQVHFMNKAANLAT
                     NADNQWMRTRSFNSRFIQDSLRADWAGELSFTPKLSITSRRYGKLFGIYREETGMAYV
                     VLFYNHEFPGFNEHLHSVFFKFPRPDKAE"
     gene            10473..10805
                     /locus_tag="PKM27_09075"
     CDS             10473..10805
                     /locus_tag="PKM27_09075"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNR07454.1"
                     /translation="MFVYNITLNIDPDTEAEGLSWIKSDFIPRVMQDGLFHEFQLCKL
                     RFADQDTPAYAVQFYCSDDSEKENLLHPGQSRYLDWVADRFGLRMMTFATVLDILEPA
                     YLSFIHKN"
     gene            10839..12005
                     /locus_tag="PKM27_09080"
     CDS             10839..12005
                     /locus_tag="PKM27_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNR07455.1"
                     /translation="MDYFKLSETQAGARDMARDFAQKILLPGVIERDRNMHFPRELIR
                     QMGDMGLMGMIVSGQYGGSGLDTLSYVLTLEEISKIDASAAVILSVNNSLVCFGLEKY
                     GTPEQKEKYLAPLAAGEWLGSFCLSEPEAGSDAMKQKTVALDKGDHYEISGIKNWITN
                     GFHSDIHLVFAQSDAALGHKGINVFIVEKSWPGITIGPKEDKMGIRASDTTSVQYDKV
                     IVPRENRLGPEGFGFTFALSTLDGGRIGIAAQALGLAAGAYDRALRYSKERETFGKPI
                     FEHQAVSFKLADMATEIEGVRLLVYRAAWLKDQGKDYTAAASMAKYAAAEIAMKQTTQ
                     AVQIHGGNGYVREYHVERMMRDAKITQIYEGTSEIQQLILSRELLKGNLYPSLY"
     gene            12005..13141
                     /locus_tag="PKM27_09085"
     CDS             12005..13141
                     /locus_tag="PKM27_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNR07456.1"
                     /translation="MFDSGTLQLAKIAGIPIRLHWSFVLIFLWVGYQGWQDRISIPQI
                     LYLEFYVITLFFCVLLHEYGHALVARKFGHKTRDILLTPIGGIARLESISEKPSQEFW
                     IAVAGPMVNIVIALLLGAFMLLSGPSEFFERAAAFPESDASFKSFLPLLMVSNVILAV
                     FNFVPAFPMDGGRILRALLSLKWSRLRSTRIAARIGQFLSLVFLIFAALQGHWMLILV
                     SLFIFFTAASELKQVQWETVLGQKNAGDLMISRFTTLDAESPMILSMELIQKGMERNF
                     IVLKDGQIFGSLSMSDIIDAMKKNGHGQPIGEYAHPNLFPVTPDQSLKKVLHLMQASG
                     HPLLPVVHMNEIAGVIDESQINKYVEIQLKAGADRMLAREKPLT"
     gene            13163..14428
                     /locus_tag="PKM27_09090"
     CDS             13163..14428
                     /locus_tag="PKM27_09090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNR07457.1"
                     /translation="METYGKILLVAMPAFLILVLFEKWYGWKKGMDTVRTMDMISSLS
                     SGVTNVVKDVLGLSVGIISYGWMVDHLAVVHVQNSLLIYMIAFLALDFSGYWVHRIAH
                     EYNFFWNNHIIHHSSEEFNLACALRQSISVFVRLFTFFLLPAALLGVPGEVIAVVAPL
                     HLFAQFWYHTRHIGKMGFLEKIIVTPSHHRVHHAINPEYLDKNYSQIFIWWDKWFGTY
                     QPELDHIPPVYGITRPVHTWNPIKINFQHLWLLIRDAWQTASWKDKLRIWIMPTGWRP
                     ADVAAKHEVYKIGDVYHFEKYETRASGALQVWTLIQLVVLLLLTSYLFGNIAAINKLN
                     PWHIYLYGLFLFVFVYAFTELMDRNPRAWIPEGIKTILGGYFLISQGDWFLASSLWPM
                     IPGTLAIYLGVSFWITVYFVRRHKLEAGG"
     gene            complement(14585..15970)
                     /locus_tag="PKM27_09095"
     CDS             complement(14585..15970)
                     /locus_tag="PKM27_09095"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNR07458.1"
                     /translation="MNKKFPEGFLWGGATSAYQIEGAWLEDGKGPSIWDVFCMIPGKV
                     INHDNGNTACDHYHRLEEDLDLIKKTGIRAYRFSISWPRILPAGREAVNPKGLEFYNR
                     LIDGLIQRDIVPWATLYHWDLPVALEMEYDGWLDARTGEAFVEYAAVCFSHFGDRVKN
                     WITINEPWVVAILGYGLGVFAPGKKSNTLPYLAGHQLILAHAKTVEVYRKQFQPVQQG
                     QIGITNNCDWREPLTDSPEDRAAAQRALEFFLGWFSDPIYKGDYPEVMKQRLGPRLPV
                     FSPEEQALIRGSSDFFGLNHYTTMYASDASGKKEAVSVYGNGGLSEDQDVHLSIGRDW
                     KLTTMQWAIVPWGFRNLLVWIAERYENPPVYITENGCAFDDQLAQGLVHDPERIEFFK
                     GYIRALAEALDSGVDVRGYFIWSLMDNFEWASGYSKKFGIIYVDQALNRIPKTSAEWY
                     RRIIETNEIGS"
     gene            complement(15993..17819)
                     /locus_tag="PKM27_09100"
     CDS             complement(15993..17819)
                     /locus_tag="PKM27_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNR07459.1"
                     /translation="MKISTLDLLIILCYLSATVVIGLLLRKRASKSKANYLLGGNTMP
                     WYLLGLSNASGMFDISGTVWLVMLLFVYGLKSVWLPWLWPVFNQIFLMIFLSIWLRRS
                     GVTTGAEWLATRFSGRGALQSHIIIVVFAIILGLGYLAYGFIGIGKFIEIFIPWEAVS
                     AYIPFDIPAEYVPHFYGICFTLFAIFYSLIGGMLSIVWADVIQYSLMAISGLVIGAIA
                     MNAIASKALIVPDGWMSPFFGYRLDLDWSRIIPEVNEKISSDGYSLFSIFFTMMLIKG
                     LLVSLAGPAPTYDMQKILSTRSPREAALMSGSVSLILMPARYFMIAGFAVLGILFYNQ
                     LDLQSGGQIDFEQILPSAISQFVPTGLMGLLLAGLIAAFMGTFAGTLNATQAYIVNDI
                     YLKFINPKADNTRIRAINWAAGLSMVFLSFILGFFAKDVNDILQWIVSAAYGSYVASN
                     VLKWYWWRFNGSGFFWGMVGGLVPALTFRFIFPGVLDLYTFPVMLALALAGAIIGTYL
                     SPPTDEAVLKAFYKNVRPWGFWKPIHDKVVAEDPSFTGNKDFKRDLFNVCIGIIWQTS
                     LVIFPIYLVLLKTVPFLVSLAIALGCSLILKRTWFDKLPKDQ"
     gene            complement(17903..19216)
                     /locus_tag="PKM27_09105"
     CDS             complement(17903..19216)
                     /locus_tag="PKM27_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR07460.1"
                     /translation="MIKRRSFLKYGSTLAGISILPFPVFGKQAPSNKVVLGVAGVNSR
                     GSWLAEYFAKLGNVEVAYLCDVEDQAIANGLKALKPSGKTPTVIRDIRKLVTQNDIDA
                     VAIATPDHWHAPAALLAVANGKHVYVEKPCGYNPGEGEMLVTASKKYKPLIQMGNQRR
                     SYPTLIEAVKRVREGLIGTPYFVKTWYTNKRKPIGTGKMVPVPSTLDFDLWQGPAPRM
                     PYKDNLVHYNWHWFWHWGTGEACNNGTHEVDCARWFLGVDFPSRVTSTGGRYAYQDDW
                     ETPDTQTAGWEFGNNKNITWEGRSCNDYPIEGSGRGFIVHGTQGTLVNDGGGSYKVYD
                     LNNKLMDHVDAKVAVDPSNPVSPAAQLDKLHLENFVETIRGNSKLNSPVEDASKSVLL
                     CHLANIAHRTGRNLQCDPRNGHILKDKGAMAFWNRKYQKGWEPVV"
     gene            complement(19256..19822)
                     /locus_tag="PKM27_09110"
     CDS             complement(19256..19822)
                     /locus_tag="PKM27_09110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HNR07461.1"
                     /translation="MKEIEKMLSGFEYNSRDPELLELYSSCKKKLHTLNTALADETDR
                     RDLLASLFGSLGEGAWIESPFYCDYGKFIRIGAHCFIHTQAVLLDSNTITLGDQVLIG
                     PGVHIYTAYHPLASKDRLRPGGAAYVTQSKPVQIGDRSWIGGGVRIMPGVNIGKDCTI
                     GAGSIVTKDIPDGVLALGNPCKVVRSIE"
     gene            19899..20951
                     /locus_tag="PKM27_09115"
     CDS             19899..20951
                     /locus_tag="PKM27_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase family protein"
                     /protein_id="HNR07462.1"
                     /translation="MKALTFSGIGTIHYEEIPDPAILDDSDVIVQTQHCAVCGSDLHV
                     FHGREVGIDPHTAMGHEFTGEVVETGKKVSRFRPGDKVVSPFTTACGSCFYCLQGLSA
                     RCIHNQIYGWVQSGAGLQGAQAEYVRVPLADSTLINYEEYDLSSELALLGGDVFSTGY
                     FGASLAEVKKGGSVVVIGCGPVGLMAVFSALLLGAGKVLAVDTIDRRLRLAGKMGAIA
                     LEAGNSLREAVHDYTGGRGADAVIEAVGSASAQSLAFELVRPGGIIATIGVHTVDHFT
                     FTPADVYNKNITYKSGRCPARSLMPTTLPLIEANGPILSEIITHRMSLKEGVRAYEIF
                     DQKRDGCIKVILHTEG"
     gene            complement(21069..22406)
                     /locus_tag="PKM27_09120"
     CDS             complement(21069..22406)
                     /locus_tag="PKM27_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR07463.1"
                     /translation="MMLTRFFKIFLACTFFISMPELNELQAQIKLKNPPPRRTKPVKK
                     PSPPPVARCSRKTDSLELVKFYNALGGPDWYNKWDLAQPVSTWHGVTTSKSGCVLEII
                     LSNNNLKGTLPNPVLPGLYQLMIDEPGVQGIIPNFNNLANLKYLILKSDLLTGGIPKF
                     DKLPELEYLDISSSQVTGTIPDLDKTPVLEELYLSGNFSGTLPDFGKLPKLHTLDITG
                     TNLEGKLPAFEKIPLLRSLRISGTKISGKIPDWAQHVHLQNLDLSDNALSDQAPSFAI
                     HSKLETLNLSGNQLAGPLPALPVSVNLSELMLQDNRFDGELKDYNMPKLRTLWLQNNN
                     LRGAVPDFKGMPELRAIDLSNNKLSGAIPNFNRLPLLENLNLAHNQLTGVIPNFVKIP
                     RLLTLQLNDNQLTGKIPEFSNLPKLMTLHFYNNPLKGKPPKFKKSPNLAEVKS"
     gene            complement(22435..23193)
                     /locus_tag="PKM27_09125"
     CDS             complement(22435..23193)
                     /locus_tag="PKM27_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNR07464.1"
                     /translation="MLGDNLKYLREIKKISQQQAAIDLGLPRTTLGDYERNHTEPSVD
                     MLAKIARYFNVGLDDLLLHKMDQIDPSRLADRDLKILAITVDSQNRQNIELVDTKAEA
                     GYLEAFQDPQYLRQLPRMYIPKLPQGSYRAFEIRGDSMLPIESGSLVIGQYVEKINEI
                     KNDHLYILIHKTEGVVFKRLKVLKEKKEIQLTSDNPAYEAYTVPFGQIGEIWQYKAHL
                     GFGDPKLALDRLADERITDIQKKVNQIYKKTIKS"
     gene            complement(23332..24036)
                     /locus_tag="PKM27_09130"
     CDS             complement(23332..24036)
                     /locus_tag="PKM27_09130"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNR07465.1"
                     /translation="MKKLTNTLLLALPFFIGNAQTKNFIDQPYLEVSGSADTLVTPNE
                     IFIKIVLGEKDTKDKIALEDLELKMVNALFALGIEVEKNLSTRDMASNYKTYLFRKKD
                     ILKSKDYILKVADANLAGKVFSSLEELGISNASIDQVDHSDLEKIKNRMRSKSVENGL
                     ERALALTIPLNQKIGTAIHIVEERGQGPGLDGRVLSEIVVTGYSKRAAASKEMPTIEF
                     EKIEIQINTRITFILK"
     gene            24386..25693
                     /locus_tag="PKM27_09135"
     CDS             24386..25693
                     /locus_tag="PKM27_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR07466.1"
                     /translation="MDRRKFVKSTAALGGASLLLDHPLQAMVHHSVADEIKIAVVGCG
                     GRGTGAAIQALSTKQNVKLVAMADAFADRIEESVKAIHKEMEESGIPKSKFAVAEENK
                     FTGFEAYKKAIPVADVVILATPPGFRPIHFAEAIAKNKQVFMEKPVATDAVGVRSVLE
                     TAEKAKAKKLNVVVGLQRHYQKVYQRWVEALQEGAIGEITASRVYWNDAGVWVKERKP
                     GMTEMEYQMRNWYYFNWLCGDHICEQHIHNLDVGNWVKGGHPVSAKGMGGRQTRTGKE
                     FGEIYDHFTIEYEYADGSRMYSQCRHTKGCLNAVTEQFDGTAGVAPRPGLIQTRKGYV
                     LLKHDDKNDANPYQVEHDVLFEAIAKNEYKFADAENGAMATLTAIMGRMAAYSGQLIE
                     WDKALHSNINLQPDAYTWDAKPKSLPDKDGFYEIPMPGKSIVI"
     gene            25811..26764
                     /locus_tag="PKM27_09140"
     CDS             25811..26764
                     /locus_tag="PKM27_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNR07467.1"
                     /translation="MGFVHLLIPLSLSFSVTAQMGRWEFHSPKMGTEFNMIVYGQDSV
                     KVARAVSLAWQRIDQVNEVFSDYSSTSELHRIHANAREKHYPVSAEFEKLLRLSLRYC
                     RLSRGAFDISIGSLSRLWRRAIKMDEFPEAEKIREALNHTGYRKIKLKDSGIRIPAGF
                     YLDFGAIAKGYAVDEAYKVLEENQLPIALLDGGGDIYAGPAPPGTRGWTVSLQVKKPD
                     NRIEDSTILVASMAIVTSGDAYKFIEHEGKRYSHIIDPRTGYGVPGPHWTTVIASSAT
                     HADALATVMSLLSGKELKKFGRKWKKRFGTQGEYWVMKFGS"
     gene            complement(26801..27658)
                     /gene="nadC"
                     /locus_tag="PKM27_09145"
     CDS             complement(26801..27658)
                     /gene="nadC"
                     /locus_tag="PKM27_09145"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNR07468.1"
                     /translation="MDQAQIESFVKLGLKEDVGEGDHTSNACIPAKSVSRARCIIKDD
                     GVVAGIEFARKAFKIIDPASRFKAFLKDGQPVVYGDIAFEVQANTRKLLMAERLILNT
                     LQRLSGIATMADRFAAEVEGLPVKILDTRKTTPLNRFLEKYAVKCGGCHNYRSGLYDW
                     IMLKDNHIDACGSIELAIRQVKEYLKKKKLKRKITIEARNLVEVYEIVRVGGVNRIMF
                     DNFQLPLLKEAVAIVNNQFETEASGGVTLLNVRKIAQTGVQYISVGALTHSAGQLDIS
                     LKIIPPEAK"
     gene            complement(27668..28546)
                     /locus_tag="PKM27_09150"
     CDS             complement(27668..28546)
                     /locus_tag="PKM27_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07469.1"
                     /translation="MCERQAGNVLSRGLKWILKRKSNQKWIFAPAEMNRNRILCLITL
                     AAWGFLPFSGHAQSLKLGLYSEPVMTWMSSTDKEIKTRGNNLGLRMGVSGEYLFHTRW
                     ALVASTNLSINQGGSLLYQTGGNFWLESELKAPNYNLGQKPVPDNSRLTYQVQFWEAG
                     LGLKYRLERHEEKNYYLEPLKMGIHKLLKARGAIHKDGLLTGGENILPDLQKISFSIG
                     GGLGQERRIGRNTWLSAGICYQWYLSDLTRNHGFKTRIVSQGNQGDPNHDIYTRLPEN
                     SKTFIHSLSLRLGFVF"
     gene            28638..29642
                     /locus_tag="PKM27_09155"
     CDS             28638..29642
                     /locus_tag="PKM27_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07470.1"
                     /translation="MRNFLILLPSLLLFQCRSGQSPKTPPVEVKVEDAFLRFIGAPED
                     QVSHRLDSILNLDRSFTKLFNGVILGATDSVISRDRVLAWFTDTSRSRLKDRCMQVME
                     SMDWQNSLGPTFARYRQAFPERTVPVIHILITDFNYGLFLFRDDLGKDAVGVGMEMFL
                     DDTPLYDQLSQQNPNFSTYLNRTFNLRHLPSKLIHILLADLIPEPPGNRLLDHIVREG
                     KILYITRALMPGIADTILHEYTDEQMRWVNENEWDIWRFLLSEKLLYQSVGKDIVNLV
                     QPAPHSQGMPPEAPGRAVNVLGLKIIEAFMKRENLSLSELAGKVDADEILQKARYRPG
                     "
     gene            29690..30646
                     /locus_tag="PKM27_09160"
     CDS             29690..30646
                     /locus_tag="PKM27_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HNR07471.1"
                     /translation="MAGKRAIPLVDLTQFTKGDEAARKSFIRKLAKAFREIGFVGVIN
                     HGIPKTLVKKFYDESNAFFNLPLEKKLKYELKGLAGQRGYTSFGKEHAKQSKVADLKE
                     FFQIGQEVAGKGAIRKEYPRNVYVRERPQFLATGLRLYKSFEKSGAQLLKAIAIHLDL
                     GEKYFDAWIKNGNSILRAIYYPPITSEPKSAIRAEQHEDINLITLLVGASAGGLQVLN
                     LDDEWLDVLPQQDEIVINVGDMLQRLTNNYLKSTTHRVINPPRSEWHKPRLSIPFFLH
                     PRSEMDLTCLPFTVTRTHPLHYPPITAGEYLAERLREIGLKK"
     gene            30739..30957
                     /locus_tag="PKM27_09165"
     CDS             30739..30957
                     /locus_tag="PKM27_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNR07472.1"
                     /translation="MNSFLLLSMPGGGEWILIFVVVLLLFGGKKIPELMKGLGKGIRE
                     FNNARNSIEEEIQEGMREVEKKKVNEPK"
     gene            31070..31570
                     /locus_tag="PKM27_09170"
     CDS             31070..31570
                     /locus_tag="PKM27_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNR07473.1"
                     /translation="MRFLFLLFIGLSPWLAQGQKFGHINSAEILSKVPEIAQADTILR
                     QYQDTLVAAGDSLVRIFQKHVAEYQAESQKGNLAPIAAQKKEEDLAKEQEVLRNYENE
                     IKQRLVLRREALYKPILDKIDQVVTRIGKENKYTMIFDSSQPGYLYLAESDNLGPQVL
                     KELGLQ"
     gene            complement(31669..32544)
                     /locus_tag="PKM27_09175"
     CDS             complement(31669..32544)
                     /locus_tag="PKM27_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR07474.1"
                     /translation="MKITIAKTTLWFLVFTASALSAQKPKVMEQNKALIRAAYEALNM
                     RNFDLFFTMVSPEFTDYTAAPMPVKGRENIQPAYEAFMKAFPDMKFTVIQTVNEGNKY
                     YTIVKLTGTNTGSVMGMLPPTGKKVEFTDVDVIEMDGAGKALSHAVSNPNLIFEQIGY
                     GFLGNSNAQAVMDIYKKFGSGDVNGILSGSDPDLMFEIDDRVFDYGTRWYKGKKEVAQ
                     FFKELNEKFKYEVFEPYDFVASGDNVYCRVKAKYTHLPSGKRYESHYTHHFKFKDGKV
                     ILFRGMDGDQSLVKM"
     gene            complement(32656..33087)
                     /gene="rpiB"
                     /locus_tag="PKM27_09180"
     CDS             complement(32656..33087)
                     /gene="rpiB"
                     /locus_tag="PKM27_09180"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNR07475.1"
                     /translation="MTIALGADHAGFEVKQAILKSLQEQNIACMDYGTYSPERVDYPD
                     FAHPVAEAVEQGAANFGILVCGSGNGVAMTANKHQGIRAALCWTEDIARLARLHNDAN
                     IIALPGRFISAEEAVRMVEIFLQTGFEGGRHSERVQKISSC"
     gene            33256..>33540
                     /locus_tag="PKM27_09185"
     CDS             33256..>33540
                     /locus_tag="PKM27_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR07476.1"
                     /translation="MKPAIFLLTLCLCVACKPELPADLILVGGNIYTGDRKNPKASAL
                     AVREGKLIYVGDEAGAKKFEGPSTERVNAGGKFVMPGFIEGHGHFAGLGGS"
     assembly_gap    33541..33640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33641..34663
                     /locus_tag="PKM27_09190"
     CDS             <33641..34663
                     /locus_tag="PKM27_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR07477.1"
                     /translation="SPEQLEKIWHDGIELAQEECLRKGVTSFQDAGSSFQEMEWYKAL
                     AEQNKLKIRLWAMLRQNSRELTSEALHTFPWIGLGNDHFTMRAVKADVDGALGSYGAW
                     LLAPYSDRSDHFYGQNTTEVSEVDAVARICIEHGLQLCVHAIGDRANREVLDLMERHF
                     EAHPENKDLRWRIEHAQHLDTADIPRFAEMGVIASMQGIHCTSDAPFVVKRLGEFRAR
                     TGAYAWKSLLRTGAVVSNGTDTPVEDVDPLASFYASVTRTRADNGMRFFSEQKMDREE
                     ALHSYTLAAAYAAFEDHTKGSLSPGKWADFILLSEDLVRCPDEAILKTEVLKTWVGGK
                     LLYSKE"
     gene            34668..>35186
                     /locus_tag="PKM27_09195"
     CDS             34668..>35186
                     /locus_tag="PKM27_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07478.1"
                     /translation="MYRWISLACLICTANPIGAQSLLRFNENSFYKPPENPILRADSN
                     FVFMDPVKQRMVRWQQADVFNPAAIVRDGKVYLLYRCEDNPVAHLGGRTSRLGLAEST
                     DGIHFTKYPQPVLYPTDDPFREYDDPGGCEDPRLVQTEDGLYVVAYTSWNQKIARLSI
                     AFSRDLIHWEKKG"
BASE COUNT         8479 a         8851 c         9188 g         8568 t
ORIGIN      
        1 gtatggttaa agaatttaaa tcggattcgt tgcggattaa aatatttgaa aatgcagagg
       61 cattggggag ggccgcttcg acggcggttg cgcaaaaact ggaggatacc atcaggctta
      121 aaggccgcgc caacctcatc ctcgctacag gagcttccca gtttgccttc ctcgaatatc
      181 ttcaaaagga aatggtggac tgggaaaaga tcaccgtatt ccacctggat gaatacctgg
      241 gcataccgga gtcccatccg gccagcttta gaaaatacct caaagaacgg attcttgatc
      301 aggtgcggcc cggccgggtt tattacctgg aaggagatgc ggccgatacg gaggccgaag
      361 ctgcaaggta tgaatcccta ttgaaggacc atcccattga cctggcctgc atcggcattg
      421 gtgaaaacgg gcacatcgct ttcaatgatc cgccggttgc ggattttaac gatccccgcc
      481 tggtaaaaaa agtggcgctg gatcaggcat gccgcctcca gcaactgggc gagggctggt
      541 tccaaagcct ggatgaggtt cccacccatg cgctcagcct gaccattccc gcgatcatgt
      601 cctgtacaag catcagttgt gtggtgcccg atgaaagaaa ggcaaaagcc gtattcggga
      661 gcgtgcatgg agaaatttcc acagcctgcc ccgcgagtat tttgcgccgg catccggata
      721 cggttttata tctcgatccg ttttcggcca aattattgtc ctgagataaa gcgttcacac
      781 agaccccgaa ccgccaaccc gaggcatgga tcggaaatta gcgggtattg gattattcgg
      841 attttaccac attcactccc agctccttca attgtttgtc atctatcgcc gaaggcgcgt
      901 cgatcatcat atcccggccc atattgtttt tcgggaaagc gatgtagtcg cggatggaag
      961 tttgtccctg catcaccgcg cacaggcggt cgaagccaaa agcgataccc ccatggggcg
     1021 gtgctccgaa ttcaaacgca ttcagtaaaa acccaaactg atcctgggcc tgttcgggag
     1081 taaacccaag cagggaaaaa tttttggatt gcaaaccccg gtcgtgaatc cggatggaac
     1141 cgccaccgat ctctgtgccg ttgatcacca aatcgtacgc gtcagcccga atgccggcaa
     1201 gcaccgcgcg gtcatcgctg gccatttttt cgatatcccc ggttttggga gaagtgaagg
     1261 ggtggtgcat ggcgaagaac cgacctgctt cttcgtccca ttccagcaag ggaaagtcta
     1321 agacccaaag cggtttgaaa tcaccggcct ggatcagaaa atgtttcctt cccagttcca
     1381 gtcgtaattc tccaagttgt ttaggggtct tgtcttgttc gccggagagg ataaacaaga
     1441 tatcgcccgg tttggcaccg gtaaaatcag cccaggcacg aagatcctcc gcgccgtaaa
     1501 acttatccac actggattta aagctcccgt cttccaggca tttgacatag atcagtcctt
     1561 tggcgccgat ctgtgggcgt tgaacccatt cggtgagttc tttgatttct ttattggaat
     1621 aatggtccgc ttctccggac agcaccaggg cgccgatgta agccgcctgg tcaaatatgg
     1681 agaaaccttt gcctttggcc ttttcattca actcatggat gggcataccg aatcgaaggt
     1741 ccggcttgtc caccccatac cgttgcatgg cctcgtcata actcatcctc gggaaggcgc
     1801 cgagatcgac ctccaacatg gacaaaaaca aatggtggat caggccctca aaagtctgga
     1861 ggatgtcttc ccggtgtaca aaggacattt cacagtcgat ctgggtaaac tccggttgcc
     1921 ggtcggcccg caggtcttcg tcccggaaac atttgacgat ctggaagtat ttgtccatcc
     1981 cggcgaccat caatatctgc ttaaaggtct gaggggactg cggcagggcg taaaactggc
     2041 cggggttgag cctgctcggg actacaaaat ccctggcgcc ttcgggagtg cttttgatca
     2101 ggaagggggt ttcgatctca ataaaaccat gtcccgacag gtatttcctg gtctccaatc
     2161 cgagcacgga acggaagatg attttggatt gaacgctgtg cctgcggatg tccaggtaac
     2221 ggtatttcat gcgcaggtca tccccgccgt cggtatcctc agttatggta aatgggggca
     2281 ccttggccgc attgagcact tccaggtttt tggccaggat ctcaatttcc ccggtaagcc
     2341 ttttcaggtt tttggcgctt cgctccctca cttcgcccgt gacccggatc acatattccc
     2401 ggcccagttt tctggcttcc tcacagaggg cggcatccga gtccatattg aagacgattt
     2461 gcgtaattcc atatcggtcc ctcaggtcca taaaggtcat gccgccaaag tcgcggctgg
     2521 catccaccca accgcttaaa gtcacggttt gccccgcatt ttcaatcctc agctctccgc
     2581 aagtatgcgt cctgtacata tctgtatggt ttggttaatt ttccgcaaag gtaaatcaat
     2641 cgggagacta tgacttctca tctgtataag aggaaagctt ccaccctttc ggcccaactg
     2701 ggaacggcca ataaatcatc cagctcatcc agatgacgga gcggttcgtt ttccaaccag
     2761 gactttaagc cttcaccacc ggacagcaat tcaaaatgcc gggcgccgga tggattgacc
     2821 agggtggggt agggtaccca cgaaaaatga tcgggatagc tggtcctcag caaggctaaa
     2881 aggatcatgc ctgtttttac cggccgaaag acagcagggt ctgtcacccg gagccggagg
     2941 ccttcgccag acagacccgg gtattgcccc tgaggttcca cgcatcgata aggcagcgct
     3001 tccatccccg gtggtgtgta caagttaaag gcatcggtca gttcttccgc ccgaatccag
     3061 ggcgcatggg ctacttcgaa tgacccgggc ccgccacggt ctacggaaac attggtagcc
     3121 tccaggaaac aaaggcatgg gtaggtcaga tagctagtgg gggaaagcag tgcaggggag
     3181 ggtggtttga aggggtatct ccagtcgaga atggtatcca ttcggttcca accttcggtt
     3241 ttgatgattt gcaaagaaag cttttccagg cccatttcat acttcaacaa caaggcaagt
     3301 tctcccatgg tgaggctgaa ccggatggga atgttccatc tccctatgaa cgaatagtag
     3361 cccggtgtga tcattgggcc ttcagcccgg gacaagtcgg cgcccagcgg attgggccgg
     3421 tccaggatga tcagcggaag tccttcttta tcgcaccatt ccatcatgta agacatggtc
     3481 cacaggtagg tataaaatcg aacgccggca tttggcaggt cgaacaggat ggcgtccagg
     3541 cctgagcgcg tctcaggcgg gggttccaat cggtctccgt aaagactggt caccggtatc
     3601 cctgaaccgg gatctgtccg gtctggctgt gcagcgccat ccacgcccct ggcctggagt
     3661 ccgtgttccg gtgaaaagag ccgggtgatc cggtatcctg ccttgagcag ggcctgtttt
     3721 accggcatgc ctgatttatt gacggcagaa tcattggtca ccaatccata aacaagggag
     3781 ggatcaggcg gacttgaggt ttccatccgg tcgattccgc ataataattc cttttccgga
     3841 gcccaggcgt ccattttgta tcttcccaaa agatttcaaa tgtatgaaat ggacctttac
     3901 ctctttactg tttgttttat ccgcatcggc ctttggccag ctttccaaaa ccgaactata
     3961 taaaaacatc gatcgacggg atgctgaata ttccggcatt gcccaaaaca tctgggatta
     4021 tgccgaagtg ggattccagg aatttaagag cagcaacctc ctcaaatcgc tcttgaaaaa
     4081 ggaagggttt tcaattcagg atagcgtcgc gggaatgccg acggcgttta ttgcgtcctt
     4141 tggaaccggt aaaccggtga tcgccctcct tggagaatac gatgcgctgc cggggctgtc
     4201 ccaggatagg gtgccttatt taaaaatatt ggacggaaaa ccggcggggc atgcctgcgg
     4261 ccatcatttg tttggaacgg cttctgccgc cgcggcgatc gaagttaaaa actggttgaa
     4321 agcatccggg accgtgggaa ccatccgatt ttatggcagc cctgccgaag aaggcggttc
     4381 aggaaaggta tacctggtga gggaaaatca gttcagggat gtggatgtgg ttttgcattg
     4441 gcatcccgcg gacgcaaaca atgcttcagc cagcccctcc ctggccaata aaaccgccaa
     4501 attcaggttc tacggggtgg cttcccatgc ggctatgtct cctgaacggg gcaggtctgc
     4561 cctggatggc gtggaggcca tgaatgccat ggtcaatttg atgagggaac acataccctc
     4621 cgatgcacgc attcattatg tcatcaccag gggcggggag gctcccaatg tagtgccggc
     4681 ctttgcggaa gtctattatt atgtgaggca ccccagggtg gagacggtgt cctccatctg
     4741 ggaaagaatt gaaaaggcgg ctctgggagc tgccatgggt acggaaacaa aggcagaatg
     4801 ggaaatcatc agcggggttt acaacctcct tcctaacgct gctatttccg gccttatgga
     4861 taaaaatctc aggatctcgg gaggatactc ctatataccg gaagaaaagg acttcgcggt
     4921 aaaaattcaa tccacattca gagggacatt gaaagacctc tccggtacct ctgcgattgc
     4981 cccatttgca gtaagcgatg tcccatggcc ggcttccacc gacgtggggg atgtcagttg
     5041 ggtggtgcct acggcaggac tgagtacggc gacctgggtg ccgggcacca cggctcacag
     5101 ctggcaggcc gtcgccgccg gaggaacctc cattggtata aaaggcatgc tggttgccgc
     5161 caaaaccctg gcaggcactg ccgtcgattt attcaacgaa agcggtttaa tcgaagaggc
     5221 ctggaaagaa ctgaaaatgc gccgtggaga accttttgaa tacaaaccga tgattggcga
     5281 ccggaagccc gcattggact ataggaattg aggaagcagt tattttttta ctaagatgac
     5341 cgggagaacc cggcgtaaaa actcctcagg gtgctagggg atccaggtcc caaagggtgg
     5401 ctttagcctg gaattgtttt ccattaattc tgaatttctg gagtgcaccc ggagcaaaaa
     5461 acacggaggt gagagccgct tttccctgat ctgcaaagat ttctaaggaa tatcggtcaa
     5521 taaggatttg aagggtgagg ttgtgtcccg ggctcacacc ggtacactca atggaagggt
     5581 aggcctggtg aaaagcaagg gatttactgg tacggtcaag acagagttgg cgacgggtcg
     5641 catcccaggt caccagcaat ttggctttct ttccaaaatc aaaatcgagt acaaccccct
     5701 tgtgggaatc caggctgatc tgccattcca ttgccgggga ggtctgtatt tcccaggtgt
     5761 caaattttgt accctgatcg gcgtggtatt ccctctcctt cttcctgatg gcctgcaggc
     5821 tttccaccgg ggcctggagg agccggtaag atccatcgga ctccctttga attccaagtt
     5881 cccgggcggt ggtcatcatg cctttccagc tttcagtcgg gttttggttg gcataggccc
     5941 agttgttcat ccatccgagc aagatctttt tttgatccgg ggactgataa aaagggatcg
     6001 cggcgtaaaa atccttgccg gcatccaccc ggttgacctg gtccagtgcg tggtcgcagc
     6061 ggaaggaggt gccgtcaaaa tcaccggtaa aatatcgcat ggatgaaaat tcccggttgg
     6121 tggaaatgag caaaacccat ttggttttgt ctttttggtt ttttaccgga agggagaaca
     6181 gggccggaca ttcccaaatt tctttgaagg aaggatggcc tccaaaatcg ctgagcaaat
     6241 cccatttgat caggtttttt gatgcataaa actgcacctt gaattccttg ggcaggacca
     6301 cggccatgat ccatcgggcg gtcggttcgt gccagaaaac attgggatcc ctgaaatcct
     6361 gttttttgag gtccagtacc ggattgtttt taaacaggtt ccaatggagg ccgtcatcgg
     6421 tgctgtatgc gatatgttgt gcctggtggc cctcccggtg agcggtgtat aaggccacca
     6481 gcggtccgcg ggaggcagac cccaggctgc ttgaattttt ctggtcatgg acgacgcagc
     6541 ctgaaaaaat catatggtct ttttgttccg ggatggctac gggcaggtgc gtccattgaa
     6601 ccaggtcctt gcttttggca tggccccagg tcatatgtcc ccattcattg ccgaagggat
     6661 tgtgctggta aaacaaatgg tattctcctt tgtaataaat caggccgttt gggtcattga
     6721 tccaaaactg tggggcagta aaatggaaaa cgggtcttgg agattccgga acgctcccct
     6781 gaaatgctga catacaaaag acagtcaaaa ttagaacaag ttctgccaat tttctcttca
     6841 cccgtttaaa gataaattca ggaatcatac attaaatcaa ttatttccgc aatttggcaa
     6901 taaaggtttt ttgttctgtt caggaagaaa gccaggcaat ttggcggaac attatttcat
     6961 aaccaattta aggttattat tgctaaaaat tcaaaacgtc actcatgtcc aaaggaattt
     7021 cttttattct tctgtctgta ttgttcctgg cttgttcgac ttcccgaaaa tcaagggtag
     7081 ccgaaatcaa gctttcgggg cccactgcta cccctgtcct tcctcaaccg gcccgggata
     7141 cacttaaacc caaagccgat acggcttctg tggaatccgg ccggcgcgct ccggctgcga
     7201 aaaacgggat aagaccttat gttgagatca tcacatccaa atcggtcagc aggccgggaa
     7261 tgttccaggt acaccaggtg gaggaccgct attattttga aattccggac tccatgatgg
     7321 gccggcccat gctggcggtc acccgttttg tccgcactcc gacctcagcc ggatatggcg
     7381 gggaattgtt aaaccggcag atgatccgtt ttgaaaaggg taccgaaaaa aaagtctttg
     7441 tccgggccac cgccctggtc aacacgggat cggatacttc agccccaatt tataaagcgg
     7501 tcctgaacag taacctggat cccattgccg ccgcttttga tatcaaggcc atccggaagg
     7561 ataccagcgt attgatcgac gtgactgact ttttctccgg ggatgttcaa atcgtcggtt
     7621 tcagcaatcc cataaagacc aggtacagga tgggcggtct tcagtccgac cgctcattta
     7681 ttcaatccat aaaaccctat cccatcaaca tcgaagtaaa aacggtgaaa acctatgcca
     7741 tttcaccgcc acctaacagc agcggcccgg gcagccctca gacggtcacc ctgccggggg
     7801 cgacggcctc gggcttcgcc tccatggaac tgaacacttc actcatccta cttcccaggg
     7861 agcccatgcg gaaaaggttg tttgatcccc gaatcggtta tttttccacg ggatatacgg
     7921 tctatgatga agagggacag cgcaccgagg atcccagttt cgcggtccgc tggaggctgg
     7981 agcccaaaaa tgaatccgac gcggagaggc agcgaagggg agaactgatc gagcccgcca
     8041 aacccatagt ttattatatc gacccggcaa ccccgaaaaa atggaggaaa tacctgatcc
     8101 agggagtcaa tgactggcag aaagcttttg aacaagccgg ttggaaaaat gccatccgcg
     8161 gggaagaatt cccggagcac gacagcacca tgagccttga agatgcccgg ttttccgcca
     8221 tccggtattt tgcctcaccg gttgaaaacg cctacggacc caatatcaat gaccccaggt
     8281 ccggggagat catcgaaagc catatcggct ggtatcacaa tgtcatgaaa ctgctcaaca
     8341 aatggtaccg aacccagact gccgcggtag accccaaggc caggccgaat gaattcgaag
     8401 atgaactgat gggtcaactg atccgctttg tagcctcgca cgaagtggga cacaccatag
     8461 ggctgcggca caatttcgga gcttccagcg ccactccggt ggagaagctc agggataaaa
     8521 aatatatata tgaaaacggc catacttctt ccatcatgga ttatgcccgg ttcaattatg
     8581 tagcccagcc cgaggacagc gtcacggacc ttttccccag gatcggagat tacgacaaat
     8641 gggccgtgga atggggatat aaacccatct atgggaccat ggatgaaaag gaagatcagc
     8701 aaatactcaa taaatggttc ctgaccaaag cctaccccaa ccggcgactg tggttcctta
     8761 ccgagatcaa tccctatgac cccagggcac agaatgaaga cctgggagac aatgccatgc
     8821 ttgcctccga gtatgggatt aaaaatctca aacggatcgt tcctcaattg ccggactgga
     8881 cccgggaaga aggccaggat tttaaggtgc ttaaagaagc ctatgaatcg gcggtggatc
     8941 agtttcggcg ctacctcggt cacgtgacca aatacgtagg gggtatttac gaaacgccca
     9001 agactttcga acaaaacagc ccggtgtatg aacctacgcc cgcccccctt cagcgggatg
     9061 cggtaaggtt tttatcccgc cacattttta acacgccgga atggctgatc gatccgtcca
     9121 ttttaaacag gatccagccc ggttccggcc tggaaaatat ccgggccatc caggaaccgg
     9181 tgatccaggg ccttttcaac gaagaccggc tgcagcgtat gatcgagacg gcggtaagaa
     9241 caccttcggc ctaccgggtg acggaccttt tccgggacct gcaatcggcc atatggtctg
     9301 atttgaatgg ttcgggtgag atcgatgtgt tccggcgcaa ccttcaaaag atatatgtgg
     9361 aaaggctgat ttccatgttg acgacgcccg ttgcaccgtc caatgcggtt acggttttcg
     9421 ggactaccct ccctgcacga acagatccaa agaaaaatga tgtgctgagc ctggtgcgcg
     9481 gccatctcaa agccttgcgt ccggtgatca tgcaggccgc agggagaagg gggtcctccg
     9541 tttccggcat gcacctcgat gaccttgccg aacggatccg taaaaccctc gaacccagat
     9601 aacgaaaaaa ggcaataaat attaatttct tttacatata agatttaaga atgaataaat
     9661 aattaaggga tcaaccggtt aaacattaat ttcaaaaata tgagttaaaa gaacccgaat
     9721 ttgtccttat ccattcaatt tggcaagaaa tatgcattat acttgccgaa gtattgatcc
     9781 taacagattg atgctttatg aggtcggttc gctggggggt aagagccgat ctcttttttc
     9841 tttttccggc ctttctcccg ccggttctca gcccagccaa atcccttaat tttgccttca
     9901 tgtcagctaa gcggttgatc ctggttttat gccttatctc cagtctccgg ggcaccgggc
     9961 agatccaata tacccgcgat tttaccgcgc ggctccgctc ggcgggcctg gaactcagcc
    10021 ttccggacag cacatggtat aaggtgcatc tgccctgtga agatgtttac aaccgttatg
    10081 atctttgcct ggtatcggaa acagattcag ccgatttaaa atatcttatc ctggatgctt
    10141 tgcaaaccaa taagatattt tctccccagg tgcattttat gaataaagcg gccaacctgg
    10201 ccaccaatgc ggacaaccaa tggatgcgga cccggtcttt taacagccgt tttatccagg
    10261 attccctaag ggcggattgg gcgggcgaac tgagttttac gcccaagttg tccatcacat
    10321 ccaggagata tggtaagctg tttggcattt accgggagga gaccgggatg gcttatgtgg
    10381 tcctgtttta caatcatgaa ttcccgggat tcaacgaaca cctgcattcg gtattcttta
    10441 aatttccccg gcccgacaaa gcggaataaa tcatgtttgt atacaacatt accctgaaca
    10501 tagatcccga taccgaagcg gaaggcctga gctggatcaa atcggacttt atcccaaggg
    10561 tgatgcagga cggcctgttt catgaattcc agttatgcaa actcaggttt gccgaccagg
    10621 atacaccggc ctatgccgta cagttttatt gttcggatga ttccgaaaaa gaaaatttgc
    10681 tccatccggg ccagtcgcgc tacctcgact gggtcgcgga ccggtttggg ctcaggatga
    10741 tgaccttcgc aaccgtcctg gatatccttg agccggccta cctctccttc attcataaaa
    10801 attgagcaat tttacacccg cttatcaaca cgcaaaaagt ggattatttc aaattatctg
    10861 aaacccaggc cggggcccgg gacatggccc gggattttgc ccaaaaaata ctgctcccag
    10921 gggtgatcga gcgcgaccgg aacatgcatt tcccccgtga actcattcgc cagatggggg
    10981 atatggggct catgggcatg attgtcagcg gacaatacgg aggcagtgga ctggataccc
    11041 tttcttatgt gcttaccctg gaggaaattt ccaaaatcga tgcgtcagcg gcagtgatcc
    11101 tctccgtcaa caattcgctg gtctgttttg gcctggaaaa atacgggacc ccggaacaga
    11161 aggagaaata cctggccccc ctggcagcgg gggaatggct cggatctttt tgcctttccg
    11221 agcccgaggc aggcagcgat gcgatgaaac aaaaaacggt agctctggac aagggagatc
    11281 attacgaaat ttcaggcata aaaaactgga ttaccaatgg tttccattcg gacatccatc
    11341 tggtctttgc ccaatccgat gccgccctgg gacataaagg gattaatgtc tttatcgtgg
    11401 aaaaatcatg gccgggcatc accataggtc caaaggaaga caaaatgggg atccgggcca
    11461 gcgataccac ttccgtccaa tacgacaagg tgatcgtgcc cagggaaaac aggcttgggc
    11521 ccgagggctt tggatttact ttcgccctga gcaccctgga tggggggcga atcgggatag
    11581 ccgcccaggc attgggcctg gcagccggag cctatgaccg ggcattgcgc tacagcaaag
    11641 agcgggaaac cttcggtaaa cccatttttg agcaccaggc ggtttcattc aaactggccg
    11701 atatggccac ggaaatcgaa ggtgtgcgcc tcctggtgta tcgcgcagcc tggcttaaag
    11761 atcagggtaa ggattatacc gctgcggcca gcatggccaa atacgcggca gccgaaatcg
    11821 ccatgaaaca aaccacccag gccgtccaga tccacggagg aaacggatat gtgagggagt
    11881 atcacgtgga acgcatgatg cgcgatgcca agatcaccca gatttatgaa ggcacttccg
    11941 agatccagca gttgatcctc tcgcgcgaat tactgaaggg caatctttat cccagccttt
    12001 attgatgttt gacagcggga ctttgcaatt ggcgaaaatc gccgggattc ccatccgtct
    12061 gcactggagt tttgtgctca tctttttgtg ggtgggatac cagggatggc aggaccggat
    12121 atccatcccg cagatcctgt atcttgaatt ttatgtaatc accttattct tctgtgtgct
    12181 tttgcatgaa tacggacatg ccctcgtggc caggaaattc gggcataaaa ccagggacat
    12241 cctcctgacc ccgattggcg ggatcgcccg ccttgaaagc atcagtgaaa aaccctccca
    12301 ggaattctgg atcgccgtgg ccgggcccat ggtcaatatt gtcattgccc tgctcctggg
    12361 cgccttcatg ttgctgtccg gcccttcgga atttttcgaa agggcggccg cttttccgga
    12421 gtcggatgcc agcttcaaat ccttcctccc gctcctcatg gtttccaatg tgatcctggc
    12481 cgtctttaac ttcgtgccgg catttcctat ggacggaggg aggatcctca gggccctttt
    12541 gtctttgaaa tggtccaggt tgagatcgac ccggattgcc gcccgcattg gccagttcct
    12601 ctccctggtc tttctgatct ttgccgcctt gcagggacat tggatgctga tcctggtgag
    12661 ccttttcatc ttttttactg cggccagtga attgaaacag gttcagtggg aaaccgtgct
    12721 tggacaaaaa aatgccggcg atctcatgat ttcgcggttt accactctgg acgctgaatc
    12781 acccatgatc ctttcgatgg aactgatcca gaaagggatg gagcggaatt ttattgtatt
    12841 gaaggacggt caaattttcg gcagcttgtc catgtccgac atcatcgatg ccatgaaaaa
    12901 aaatggccat gggcagccga tcggggagta tgcgcatccc aacctgtttc cggtgacccc
    12961 cgatcaaagc ctcaaaaaag tccttcacct gatgcaggct tccggccatc cccttctgcc
    13021 cgtcgtacat atgaatgaaa ttgcaggggt gatcgatgaa agccagatca ataaatatgt
    13081 tgaaatccag cttaaagccg gggcggaccg catgctggcc cgggaaaaac ctttaacttg
    13141 atctgcgttt gatccgtttc gtatggagac ctatggtaaa atattgttgg tggccatgcc
    13201 ggcctttctg atcctggtct tgtttgaaaa atggtatggc tggaagaagg gcatggatac
    13261 cgtcaggacc atggatatga tatcttccct gagttccggg gtgaccaatg tcgtaaagga
    13321 tgtgcttggg ctgagtgtgg gcattatcag ctatggctgg atggtggacc acctggccgt
    13381 cgtccatgtt caaaactccc tgctgattta tatgatagcc ttcctggccc tggatttttc
    13441 cggttactgg gtgcaccgca tcgcgcacga atacaatttt ttctggaaca accacatcat
    13501 ccatcacagc agcgaggagt tcaatcttgc ctgcgccctc aggcagagca tttcggtttt
    13561 tgtaaggttg tttacttttt tcctccttcc tgccgccctg ctcggagtac cgggtgaagt
    13621 gattgccgtc gtggctcccc tgcacctttt tgcccagttc tggtaccata cccgccatat
    13681 cggcaagatg ggattcctgg aaaagatcat tgtgacccca tcccatcacc gggttcatca
    13741 cgccatcaac ccggaatacc tggacaagaa ttattcacaa atcttcatct ggtgggacaa
    13801 atggtttggc acttatcaac ccgagctgga ccatatccct cccgtatacg gcattacccg
    13861 tccggtacat acctggaacc cgatcaaaat caatttccag catctttggc tgctgatccg
    13921 ggatgcatgg caaacggcca gctggaagga taaactgcga atctggatca tgcccaccgg
    13981 ctggcgtccc gcggatgtgg ccgctaaaca cgaggtgtat aagatcggag atgtatacca
    14041 ttttgaaaag tacgagaccc gggcgtcggg cgccctgcag gtctggacct tgatccagct
    14101 cgtggtcctg cttttactca ccagctattt attcggcaat atcgcagcca tcaacaaact
    14161 gaatccctgg catatttacc tctatggcct ttttttattt gtatttgttt atgcctttac
    14221 tgaactcatg gaccggaatc cacgggcctg gatcccggaa ggcatcaaaa caatactggg
    14281 cggctatttt ctgatctccc agggagattg gttcctcgcc tcttcgttgt ggcccatgat
    14341 ccctggtacc ctggccattt acctgggggt ttcgttttgg atcacggtgt actttgtaag
    14401 aagacataag ttggaagctg ggggctagaa gttggaagct aggagctaaa tgttagaagc
    14461 tggaagttag aagttaggaa agttttgtcc tggccgttcc tggaatttga gtggttcaca
    14521 cccagctacc agcttcgagc ttcgagatac cagcttcgag cttcgagcta ccagctacca
    14581 gcccctagct cccaatctca ttggtttcaa tgatccggcg ataccactct gctgaggttt
    14641 ttgggatccg gttcagcgcc tggtccacat agataatccc gaatttttta ctatacccgg
    14701 aggcccattc gaaattgtcc atcagggacc agataaaata accgcggacg tccacaccgg
    14761 aatccagggc ctctgccagg gcacgtatat atcctttaaa aaattcgatt cgctcagggt
    14821 catggaccag tccttgggca agctggtcgt cgaaggcgca gccgttttcc gtgatgtaaa
    14881 cgggtgggtt ttcgtatcgt tcggcgatcc acaccaacaa gttcctgaaa ccccagggca
    14941 cgatggccca ttgcatggtg gtgagtttcc agtcccgtcc gatgctgaga tggacatcct
    15001 ggtcctcgga caagccccca ttgccataaa ccgaaacggc ctccttctta cccgacgcgt
    15061 cggaagcata catcgtggtg tagtgattga gtccgaaaaa atcggaggat ccgcggatca
    15121 atgcctgttc ttcgggacta aaaaccggaa gtctcgggcc aagcctttgt ttcatcacct
    15181 ccggatagtc ccccttgtaa atgggatcgg aaaaccagcc caggaaaaat tcaagggccc
    15241 tctgggctgc ggcccggtct tccggtgaat cggtaagggg ttccctccag tcacagttat
    15301 tggtaattcc gatttgccct tgttgaaccg gctggaactg ttttcgatat acctctacgg
    15361 ttttggcatg ggcgaggatc agttggtgtc ctgccagata agggagggta ttcgattttt
    15421 ttcccggggc gaaaactccc agcccataac ccaggatggc aaccacccag ggctcgttga
    15481 tggtgatcca gttttttacc cggtctccga aatggctgaa acaaaccgcg gcatattcca
    15541 caaaggcttc tccggtcctg gcatccagcc agccatcgta ttccatctcc agggcgacag
    15601 gcaggtccca atggtacagg gtggcccagg ggacgatgtc cctttgaatc aaaccatcga
    15661 tgagccggtt gtaaaattcg aggccctttg gattgactgc ttcccggccg gccggaagaa
    15721 tccggggcca ggagatcgaa aagcggtaag cccggatccc ggttttttta ataaggtcca
    15781 ggtcttcctc cagccggtgg taatgatcgc aggcggtatt gccgttgtcg tggttgatca
    15841 ccttaccggg gatcatacag aatacatccc agatcgaagg gcctttgcca tcctcaagcc
    15901 aggcgccttc tatctggtag gcagaggtgg cccctcccca taaaaatcct tccggaaatt
    15961 ttttattcat ggcaggaaat gatcttgggg cctcattgat ctttgggcag tttatcaaac
    16021 caggtgcgtt tgaggatcag gctgcacccg agcgcgatag ccaggcttac gaggaaaggg
    16081 acggttttca ataatacaag gtaaataggg aatataacca aggaagtctg ccagatgatg
    16141 ccaatacaca cattgaacag gtcccgtttg aaatccttgt ttccggtaaa ggacgggtct
    16201 tcggccacca ctttgtcgtg tatgggtttc cagaaccccc agggcctgac gtttttgtaa
    16261 aaggccttca gcacggcctc gtcggtcgga ggcgacaggt aggtgccgat gatcgcgccg
    16321 gccagtgcca gggccagcat gaccggaaaa gtgtacaagt ccaaaactcc ggggaatata
    16381 aagcggaagg taagggccgg gaccagtccg ccaaccatgc cccagaaaaa gccgctgcca
    16441 ttgaacctcc accaatacca cttgagtaca ttggaagcca cataggaccc ataagccgcg
    16501 gagacgatcc attgcaggat gtcgttgacg tccttggcaa aaaagccaag gataaaactg
    16561 aggaatacca tggaaaggcc tgcggcccag ttgatggccc tgatccgcgt attgtccgct
    16621 tttgggttga tgaatttcag gtagatgtcg tttacgatat aagcctgagt ggcgttgagg
    16681 gtgccggcaa aggtgcccat gaatgctgca ataagtccgg ccaggagaag gcccatcaat
    16741 ccggtgggca caaactggct gatggccgaa ggaaggattt gttcaaaatc aatctggcct
    16801 ccggactgga ggtcaagctg gttgtaaaac aggatgccca acacggcaaa gccggcaatc
    16861 ataaaataac gggccggcat caggatcagg cttaccgagc cgctcatcag ggccgcctcc
    16921 ctgggggagc gcgtggaaag gattttctgc atgtcgtagg tgggggcggg accggctaag
    16981 gaaaccagga gtccttttat cagcatcatg gtaaaaaata tgctgaagag cgaatagccg
    17041 tcgctgctga ttttttcatt gacttccggg atgatccggc tccagtccag gtcaagccgg
    17101 tatccaaaaa acgggctcat ccatccatcc ggtacgatca gggctttgga cgcaatggca
    17161 ttcatggcga tggccccgat caccaatccg gaaatcgcca tcaggctgta ctggatcaca
    17221 tcggcccata caatgctcag catgccccca atgagggaat aaaaaatggc aaagagggta
    17281 aaacaaatcc cgtaaaagtg ggggacgtac tctgcgggga tatcaaaagg gatataggcg
    17341 gatacggcct cccagggaat aaaaatttca atgaatttgc cgataccgat aaaaccatag
    17401 gccaggtaac ccaatcccag gataatggca aagaccacaa tgatgatatg ggattgcagg
    17461 gctccccgtc cgctgaaccg ggtcgccagc cattcggcac cggtcgtcac ccctgaacgc
    17521 ctgagccaga tggacaaaaa gatcatcagg aagatctggt tgaacaccgg ccaaagccag
    17581 ggcaaccaga cgcttttgag cccgtataca aaaagcagca tgaccagcca taccgttcca
    17641 ctgatgtcga acatgcccga tgcattcgac agaccaagga gataccaggg catggtgttg
    17701 cccccaagca ggtagttggc cttgcttttc gacgcccgct tacgcaagag cagcccaatg
    17761 accaccgtcg cgctcagata acataaaatg atgagcaggt cgagggttga aattttcatg
    17821 atggattttt gggtttaacg gtccgaaatg aatcgaaggg ggtcatcaaa aggatgaagt
    17881 gatcatgcga ttccaattca ggtcagacta ccggttccca tcctttttga tattttctgt
    17941 tccaaaaagc catggcgcct ttgtctttca aaatatggcc gttgcgggga tcgcattgca
    18001 gattccgtcc cgtgcggtgg gcaatatttg caaggtggca caacaatacg cttttggacg
    18061 cgtcttcgac cggggaattg agtttggaat tgccacggat cgtttccacg aaattttcca
    18121 ggtgcagttt atccagttgg gcggcagggc tgaccggatt ggagggatcc acggccactt
    18181 tagcatccac atggtccatg agtttattgt tcagatcata aaccttgtag gatcccccgc
    18241 cgtcattgac cagggtgccc tgggtgccgt gaacaatgaa gcccctgccg ctgccctcga
    18301 tgggatagtc gttgcagctt cttccttccc aggtgatgtt tttgttgttc ccgaattccc
    18361 agccggcggt ttgtgtatcc ggggtctccc agtcgtcttg gtatgcatac ctgccgccgg
    18421 tcgaggttac ccggctcggg aaatcaacgc ccaggaacca ccgggcgcaa tcgacttcat
    18481 gggttccgtt gttacaggct tccccggtac cccaatgcca gaaccaatgc cagttgtaat
    18541 gcacgagatt gtctttgtag ggcatgcggg gcgcgggtcc ctgccagaga tcaaaatcca
    18601 gggtagaagg tacgggaacc attttaccgg ttccgatggg cttcctttta ttggtatacc
    18661 aggttttcac aaaataaggg gtgccgatca gtccctctct cacccgcttg acggcctcga
    18721 tcagggtagg ataggacctg cgctggttgc ccatctggat caggggcttg tattttttgg
    18781 aggccgtcac cagcatttct ccttctccgg gattgtatcc gcagggtttt tcgacataga
    18841 catgttttcc gttggcgacg gcaaggaggg cggccggggc atgccagtgg tccggggtgg
    18901 caatggccac ggcatcgatg tcgttttgag tgaccagctt gcggatatcc cggatcaccg
    18961 tcggcgtctt gcctgaaggt ttcagggcct tcaagccatt ggcaatggcc tggtcttcca
    19021 cgtcgcagag gtaggctacc tccacattgc ccaatttggc aaaatattcc gccagccagg
    19081 aaccgcggct gttgacgccg gccacgccga ggaccacttt attggatgga gcttgtttac
    19141 caaataccgg aaagggtaaa atacttatcc cggccagggt gcttccgtat ttcaaaaagg
    19201 atcttctttt aatcatggat gcaggcgttg aaaggtgaga ggctaatata atactttatt
    19261 caatcgaacg gaccacttta cagggattgc ccagggccaa aacaccatcc ggaatgtcct
    19321 tggtcactat gctgccggcg cctatagtac aatcttttcc aatattgaca ccaggcatga
    19381 tccggacgcc tccgccgatc cacgaccggt ctccgatctg caccggttta ctctgcgtga
    19441 cataagctgc gccgcccggc cgcagacggt cttttgaggc cagggggtga taggcggtat
    19501 aaatatgtac ccccgggcca atcaatacct ggtctcccag ggtaatggta ttgctgtcga
    19561 gcaaaaccgc ctgggtgtgg ataaaacaat gggctcctat ccggatgaat ttgccgtaat
    19621 cgcaataaaa gggactttcg atccaggctc cctcacccag gctgccaaac agggaggcca
    19681 acaagtccct ccggtccgtt tcatccgcaa gggcggtatt gagggtgtgc aatttttttt
    19741 tgcacgaaga atagagttcg agcaattccg ggtcgcggct gttgtattca aacccggaca
    19801 gcattttttc aatttctttc atagaacaga taaaataaag aaatagcttt aattccttaa
    19861 ttctttaatc tattaattcc ttaattcctt aattcaacat gaaggccctc acctttagcg
    19921 gcataggcac gatccattat gaagagatcc cggatccggc catactcgat gacagcgacg
    19981 tcatagtcca aacccagcac tgtgcggtct gcggctctga tctccatgtt tttcatggca
    20041 gggaggtggg cattgatccc catacggcga tgggccatga atttaccggg gaggtggtgg
    20101 aaacaggtaa aaaagtcagc cggttccgcc cgggagataa agtagtcagt ccctttacca
    20161 cggcctgcgg gtcctgcttt tattgcctgc agggactgag tgcgcgctgc atccacaacc
    20221 agatctatgg ctgggtgcaa agcggggcgg gtttgcaggg cgcccaggcg gaatatgtga
    20281 gggttcccct ggcggattcg accctgatca attacgaaga atatgactta agcagtgaac
    20341 ttgccctgct tggcggagat gtattcagta ccggctattt tggggccagc ctggcggaag
    20401 tgaaaaaagg cggctcggtg gtggtgatcg gatgtggtcc ggttggcctg atggcggtat
    20461 tcagcgccct gttgttgggg gcaggcaagg tcttggccgt ggataccatc gaccgccgtc
    20521 tccggctggc cggtaaaatg ggagccatcg ccctggaagc cggcaattct ctgcgggaag
    20581 cggttcatga ttataccggg ggcaggggag cggatgccgt gatcgaagcc gtagggagcg
    20641 catctgccca aagccttgct tttgaattgg ttagaccagg cggaatcatc gcgacgatcg
    20701 gggtgcatac cgtcgaccat tttacgttta caccagccga cgtatacaat aagaacatca
    20761 cttataaaag tggccgctgt ccggcccgct ccctgatgcc caccaccttg cccctgatcg
    20821 aagccaatgg tccaatactt tcagagatca ttacccaccg catgagcctg aaggagggag
    20881 tgcgtgctta tgagattttt gaccagaaaa gggacggttg tattaaggtg atcctccaca
    20941 cggagggctg agggttcttt gttaaatttc aagccaccca ggctaaaagc caaatggttc
    21001 cgttgcaatg gtatccctgc agttaaagat gcgggtccag cgttgcccat gggagcggtg
    21061 ttcccgcatc aacttttgac ttccgcgagg ttgggagatt ttttgaattt tgggggtttc
    21121 cctttcagag gattattgta aaaatgcagg gtcatgagtt ttggcaagtt gctgaattcc
    21181 ggaattttgc ctgtcagttg attgtcatta agctgaaggg tcagcaggcg tggtattttg
    21241 acaaaattgg gaatgacccc ggtcagctgg ttatgcgcca ggttgaggtt ttccaaaaga
    21301 ggtaagcgat taaaattagg aatggccccg ctgagtttat tgttgctcag atctattgcc
    21361 cggagttcgg gcatcccttt aaaatcaggg accgctccgc gaaggttgtt gttttgcagc
    21421 cacaaggtcc gcaatttggg catattatag tctttcagtt ctccgtcgaa tcggttgtcc
    21481 tggagcatca gctctgagag gttcaccgag acaggtaaag ccggcagggg gcctgcaagc
    21541 tgattgccgg ataaattgag ggtttccagt ttgctgtgga tcgcgaagga gggcgcctga
    21601 tcactaaggg cattatcgga aaggtccagg ttttgcagat gaacatgctg ggcccaatcc
    21661 ggaatttttc ctgaaatttt tgtgccggat atcctgaggg accgcagcaa gggaattttt
    21721 tcaaaagcag ggagcttgcc ttccaggttg gtgcccgtga tatcaagggt atgcagtttg
    21781 ggaagttttc cgaaatccgg caaggtccct gaaaaattcc cgctgagata aagttcttcc
    21841 aggactgggg ttttatccag atcggggatc gtcccggtga cctggctgga agagatgtcc
    21901 aggtattcca gttcgggcag tttatcaaat tttggtatgc cgccggtcag gagatcggat
    21961 ttgaggatca gatacttgag gttggccagg ttgttgaaat tgggaatgat tccctgcaca
    22021 ccgggctcgt cgatcatcag ttgataaagt ccgggcagga ccggattggg aagggttcct
    22081 ttgaggttgt tgttggacag gatgatttcc aggacgcaac cggacttgga ggtggttact
    22141 ccgtgccagg tgctgacggg ttgggcaagg tcccatttgt tgtaccagtc cgggcctccg
    22201 agggcgttgt agaatttgac gagttcgagg gaatcggttt ttcttgaaca tcgggcaacc
    22261 ggcggtgggc tgggtttttt aacgggtttg gtccgtcttg ggggaggatt tttaagtttt
    22321 atctgggcct gcaactcatt cagttccggc atgctgataa aaaaagtaca ggctaagaat
    22381 attttaaaga atctggtcaa catcatttgg atttgatggg ataaaagacg gggttcagct
    22441 tttaatggtt tttttataga tctggttcac ttttttctga atatcggtga tccgttcatc
    22501 ggccagccgg tccagggcca gtttcgggtc cccgaaacca aggtgtgcct tgtattgcca
    22561 gatttcgccg atttggccaa aggggacggt ataggcctca taagccgggt tgtccgaggt
    22621 caactggatc tctttttttt ctttcaggac tttcaggcgc ttgaatacca cgccctccgt
    22681 cttgtgaatc aggatgtaaa gatggtcatt tttgatttca tttatttttt ccacgtattg
    22741 gccgatgacg agggaaccgc tttcgatggg aagcatggaa tctccccgga tctcgaaagc
    22801 ccgatagctg ccctggggta gtttcggaat atacatccgg ggaagctggc ggaggtattg
    22861 cggatcctgg aaggcctcca ggtacccggc ttccgctttg gtatccacca gttcgatatt
    22921 ctggcggttt tggctgtcca cggtgatcgc caggatttta aggtcgcggt ccgcgagacg
    22981 ggagggatcg atctggtcca ttttatgcag gagcaggtca tccagtccaa cattgaagta
    23041 gcgggctatt ttggcaagca tgtccacgct gggctcggta tgattccttt cataatcgcc
    23101 cagggtggtg cgtgggaggc ccaggtcaat ggcggcctgc tgttgcgaaa tttttttgat
    23161 ttccctcagg tatttcaggt tgtcacccag catgattccg gatttttcga caaagttagg
    23221 gataattttt cgactgtatt tgcccggccc caagtaggta aaggtgttgc aaggaatggg
    23281 atgccatgat gacaggcacc cacccattgg ttgaatagga atgatgtgac tttatttaag
    23341 tataaacgta atccttgtat tgatctggat ttctattttt tcaaattcta ttgttggcat
    23401 ttctttggag gccgctgccc tttttgaata acccgtaacc acaatttcgc taagcaccct
    23461 tccatcgaga cctgggcctt gtccccgctc ctctacaatg tgaatggcag ttccgatttt
    23521 ctgatttaag gggatggtca aagccagagc cctttccaaa ccgttttcga ccgattttga
    23581 ccgcatccgg tttttgattt tttccaagtc cgaatggtcg acctggtcga tggatgcatt
    23641 ggaaatgccc agttcttcca aacttgaaaa tactttgccg gcaaggttgg catcggccac
    23701 cttaagaatg tagtcttttg atttcaagat gtccttttta cggaatagat aggtcttgta
    23761 gttgcttgcc atatcccgcg tactcaggtt tttttcaact tctattccca aggcaaacaa
    23821 ggcatttacc attttgagct caagatcttc cagtgcaatt ttgtctttgg tgtctttttc
    23881 acctaaaaca atttttataa atatttcatt cggcgtcacc aaggtgtccg cactgcctga
    23941 tacctccaga tagggctggt caataaaatt ttttgtttgc gcatttccaa tgaagaaggg
    24001 taaggccaac agcaaggtat ttgtgagttt tttcatattg ttgggtgttt aaggaatttg
    24061 gacatttaaa gatataacga tggaaggctt tgtccgcgta acgaaacttg tatataaatt
    24121 taaatacatt caccctgggc ggctgtcacc gcagatttta tccctgccca aaagcccttc
    24181 atgcgcatga ccttaccatt gttttattat aatcttccgg ttttctgctt aacagtatcc
    24241 tgatcttgtt tacaatggct atatcctata gaacggcatc cttacagtat tcaaaatccg
    24301 aagaaatagc caggccgtcc aacacccttg gtttccaccc gcattctccc tatttttatc
    24361 attcactaaa aacaaaaact gcatcatgga tcgtcgaaaa tttgtaaagt ccaccgctgc
    24421 cctgggcgga gccagcctgc tgctggacca tcctctccag gccatggttc accacagcgt
    24481 ggccgatgaa atcaaaatag ccgttgtggg ctgcgggggc aggggtaccg gcgccgcaat
    24541 ccaggccctg agcaccaaac aaaatgtaaa gctggtcgcc atggccgatg ctttcgccga
    24601 ccgcatcgag gaaagcgtga aagccattca taaggaaatg gaagaatccg gcatccccaa
    24661 atcaaaattt gccgtcgccg aagaaaacaa attcaccggc tttgaagctt ataaaaaggc
    24721 catccccgtg gccgatgtgg tgatcctggc cacccctccg ggattccgtc ccatccattt
    24781 tgcggaagcc atcgccaaaa acaaacaagt cttcatggag aagccggtcg ccaccgatgc
    24841 cgtgggcgtc cggagcgtcc tggaaaccgc cgaaaaggcc aaggcaaaaa aactgaatgt
    24901 ggtcgtcggc ctccagaggc actaccagaa agtttatcaa agatgggtgg aggccctgca
    24961 ggaaggcgcc atcggcgaga tcactgcgtc gagagtttac tggaatgatg cgggggtatg
    25021 ggttaaagaa cgcaagccgg gcatgacgga aatggagtac cagatgcgca actggtatta
    25081 tttcaattgg ctgtgcggag accacatctg tgaacagcat atccacaact tagatgtggg
    25141 caactgggta aaaggaggcc atcccgtctc ggccaaaggc atgggcggcc gccagacccg
    25201 caccggcaag gaattcgggg agatctacga tcatttcacc attgaatatg aatatgccga
    25261 cgggtcgcgg atgtatagcc aatgccgcca tacgaaaggt tgcctcaacg ccgtcacgga
    25321 acagtttgac gggactgccg gcgtggcccc aagacccggc ctcatccaga cccgcaaagg
    25381 gtatgtgctc ttgaaacacg acgataaaaa tgatgctaat ccttaccagg tggagcacga
    25441 tgtcctcttt gaggcaattg ccaaaaatga gtacaaattt gccgatgcgg agaacggagc
    25501 catggccacc cttacggcca tcatgggcag gatggcggct tacagcggcc agctgattga
    25561 atgggacaaa gcgctgcatt ccaacatcaa cctgcaaccg gacgcctaca cctgggatgc
    25621 caaacccaag tccttgccgg ataaagacgg attttacgag atcccgatgc cggggaaatc
    25681 gattgttatt taaataaagg agaaagggag gaagggagaa aaggaggaag ggaacgctct
    25741 ccttcccgat gttccgatgc acaccaggga cccctaagag tgggtctcaa tattatcttt
    25801 ccgaattaag atgggatttg tacatctgtt gattccgctc agtttgagct tttccgtcac
    25861 ggcacaaatg gggcgttggg aattccattc gcccaaaatg ggcactgaat tcaatatgat
    25921 cgtttatggt caggattccg tcaaagtggc aagggcggta agccttgcct ggcagaggat
    25981 tgaccaggtc aatgaggtct tcagcgatta cagttccacc agcgaattac accggattca
    26041 tgcaaatgcg agggagaagc attacccggt cagcgcagag tttgaaaaac tccttcgcct
    26101 gagcttgcgg tattgccgtc tgagccgggg cgcatttgac atctctatcg gctcattatc
    26161 caggctttgg cggagggcca taaaaatgga tgaatttcct gaggctgaga aaatcaggga
    26221 agccctgaac cataccgggt accggaaaat aaaattaaaa gattcaggca tccgcattcc
    26281 ggccgggttc taccttgatt ttggcgccat tgccaagggt tatgcggtgg acgaggcata
    26341 caaggttctc gaggaaaatc aactgcccat cgccctgttg gatggtgggg gagatattta
    26401 tgccggaccc gccccacccg ggacgcgggg ctggacggtc agcctgcagg taaagaaacc
    26461 ggataatcgt attgaagaca gtaccatcct ggtcgcctca atggccattg tgacctcggg
    26521 ggatgcctat aaatttatcg aacatgaggg gaaacggtat tcccatatca tcgacccccg
    26581 caccggctac ggcgtccccg gtccgcattg gaccaccgtg atcgcttctt cagcaacgca
    26641 tgcggatgca ctggctactg tcatgagcct cttgtccggt aaagagctta aaaaattcgg
    26701 gagaaaatgg aaaaagcgtt ttgggacgca gggggaatac tgggtaatga agttcggaag
    26761 ttagtaagtt ggcaagttag taagttagta agttggcgtg ctatttcgct tccggaggta
    26821 tgattttcaa actgatatcc agttgtcctg cagagtgggt caaggcgccc accgaaatat
    26881 actgcacccc ggtctgcgct attttcctca cgttcagcag ggtgactccc ccggacgctt
    26941 ccgtttcaaa ctggttattg acaatggcca cggcctcctt gagcaggggc aactgaaaat
    27001 tgtcaaacat gatccggttt actcccccta cgcggacaat ctcataaacc tccaccaggt
    27061 tgcgggcttc gatggtgatc tttcttttga gttttttctt tttgaggtat tctttcacct
    27121 gccggatggc cagttcaatg gaaccgcagg catcgatgtg gttgtccttg agcatgatcc
    27181 aatcatagag gccgctgcgg taattgtggc agccgccgca tttgaccgcg tatttttcga
    27241 ggaagcggtt gagcggagtg gtttttcgtg tatccaggat cttgaccggc aatccttcca
    27301 cttcagcggc aaaacggtcc gccatggtgg caatcccgct caggcgctgc agggtattca
    27361 ggatcagccg ttccgccatg agcaatttac gggtattggc ctgtacctcg aaagcgatat
    27421 cgccgtaaac cacgggctgg ccatctttca gaaatgcttt gaaccgggaa gccggatcga
    27481 tgatcttaaa ggccttgcgt gcaaattcaa tgcctgccac cacgccgtcg tccttaatga
    27541 tgcaacgggc cctggatacc gacttggcgg gaatacaggc attggaggta tgatctcctt
    27601 cccccacatc ttcttttaag cccagtttta caaatgattc gatttgcgct tgatccatac
    27661 ctctgtttta aaatacaaag cccagccgga gactcaggct gtgaataaac gtttttgaat
    27721 tttccggcaa gcgggtgtag atatcgtggt ttggatcacc ctggtttccc tgggatacga
    27781 tccgggtctt gaatccatgg ttgcgggtga gatcggagag gtaccattga taacagatac
    27841 cggcagaaag ccaggtgttc cttccgatcc ttctttcctg gcccaaaccc cctccaatgg
    27901 aaaaggatat tttttgcaaa tcgggcagaa tgttttctcc cccggtcaac aagccatctt
    27961 tgtgtatggc gccccgcgcc ttgagtaatt tatgaatacc catcttcagt ggctcaagat
    28021 aatagttttt ctcttcgtgc ctttccaggc ggtatttcag gcccaggccg gcctcccaga
    28081 attgaacctg gtaggtgagg cggctgttat ccggcaccgg cttttgtccc aggttatagt
    28141 tcggtgcttt aagttctgat tccagccaga aattgcctcc ggtttgatag agcagggacc
    28201 ctccctggtt gatggaaagg ttggtagacg ctacaagggc ccatcgggtg tgaaaaaggt
    28261 attcgccgga aacgcccatc ctgaggccaa gattattacc cctggttttt atttccttat
    28321 ccgtggaaga catccaggtc atcaccggtt cggaatacag tcccagtttg agagattggg
    28381 catgcccgga gaatggaagg aatccccagg cagccagggt gatcaaacat agaatccggt
    28441 tccggttcat ttcagcgggc gcaaatatcc acttttgatt tgattttcgc tttaatatcc
    28501 atttcaggcc ccggctgagc acattccccg cttgtcgttc acacatatca ttataacgtg
    28561 tgttttcgtt aattgcctga gaagaccata gttaaataga ttaattaatt tcgccgccat
    28621 aagtcccgcg ccccgacatg agaaacttcc tgatcctcct ccccagcctt cttttatttc
    28681 agtgccgttc cgggcaaagc cccaaaacgc cccccgttga agtgaaggtt gaagacgcat
    28741 tcctccggtt tattggagcg ccggaggacc aggtctccca ccgcctggat tcgatcctca
    28801 acctcgatcg gtcatttacc aaacttttca atggggtcat tcttggagcg acggacagcg
    28861 tgatctcgcg cgaccgggtc ctcgcatggt ttacggacac ttcgcgatca aggcttaagg
    28921 accgctgcat gcaggtcatg gagtccatgg actggcaaaa cagcctggga ccgacctttg
    28981 caagataccg gcaggccttt cctgaaagga cagtcccggt gatccatatc ctgatcaccg
    29041 attttaatta cgggctattc ttattcaggg acgacctggg aaaggacgct gtgggagtgg
    29101 gcatggaaat gtttctcgat gataccccgt tatacgatca gctttctcag caaaatccaa
    29161 atttttccac ttacctcaac aggaccttca acctgagaca cctaccttcc aaactgatac
    29221 atatcctgct tgccgacctg atcccggagc ctcccggcaa ccgattgctg gaccacatcg
    29281 tccgggaagg gaaaattctc tatatcacca gggccctgat gcccggtatt gccgatacca
    29341 tcctgcatga atataccgac gaacagatgc gttgggtgaa tgaaaatgaa tgggacatct
    29401 ggcgcttcct cctgagtgaa aaactgctct accaatccgt gggaaaagat atcgtaaatc
    29461 tggtgcagcc tgcgccgcat agccagggca tgcccccgga agccccgggc cgcgcggtca
    29521 atgtgctggg cctcaaaatc attgaggctt ttatgaagcg ggaaaacctc agcctctccg
    29581 aactggccgg gaaggtcgac gcggatgaaa tcctgcaaaa agcccggtac cgcccgggct
    29641 aagagccgaa tggaacggaa tttgtatatt acaaaaaaat ttaatttgta tggccggaaa
    29701 aagagcgatc cccctggtgg atcttaccca gtttaccaaa ggggatgagg cagcgcgcaa
    29761 atccttcatc cggaaacttg caaaagcctt tcgtgaaatc ggttttgtag gggtcatcaa
    29821 tcatgggatc cccaaaaccc tggtcaagaa gttttatgac gaatccaacg cattttttaa
    29881 cctgcccctg gaaaagaagc tgaaatatga attaaaaggc cttgcaggtc agcgggggta
    29941 cacctctttt ggaaaggaac atgccaaaca atctaaagtc gccgacctta aggaattttt
    30001 ccagataggc caggaggtgg ccggcaaagg ggctatccgt aaagaatatc ccaggaatgt
    30061 gtatgtccgg gaacgtccgc aattccttgc aaccggactc aggttgtaca agtcctttga
    30121 aaaatccgga gcccagttgt tgaaagcgat tgcaatccac ctcgacctgg gtgaaaaata
    30181 ttttgatgcc tggatcaaaa acgggaacag catcctccgg gccatctatt atcctccgat
    30241 cacctctgaa cccaaatcgg cgatccgggc agagcaacac gaagacatca acctgatcac
    30301 cctcctcgtc ggcgcttcgg caggcggcct ccaggtgctc aaccttgacg atgaatggct
    30361 ggacgtattg cctcaacagg atgaaatcgt gatcaatgtc ggggacatgc ttcagcggct
    30421 caccaacaat tacctgaaat ccaccaccca ccgggtgatc aatccgccca ggtcggaatg
    30481 gcacaaaccc aggctgtcca tcccgttttt ccttcatccc cggagcgaaa tggacctgac
    30541 ctgtctgccc tttaccgtca ccaggaccca tccgctccac tatccgccca ttaccgctgg
    30601 ggaatacctg gcggaaaggt tgagggagat cgggcttaaa aagtaaccag ggaccggttt
    30661 ggctgaaata taagctttgg ttaaagactt cccactgcgc ttaaattctc cgtatatttg
    30721 tctcaaattg caacaataat gaactcattt ttattattat caatgcccgg cggaggtgaa
    30781 tggattctga tcttcgtagt ggttctattg ctctttggtg gcaaaaaaat tccggagttg
    30841 atgaaaggcc tgggcaaagg aatcagggaa ttcaacaatg cgcgtaattc catagaggaa
    30901 gaaatccagg aaggaatgcg cgaagtggaa aagaaaaaag taaacgaacc caaataaatt
    30961 ccagtcgggg attttaaata aaataccctg gggccatgct ccggggtttc tttttataag
    31021 ctgtaacttt taaattttgc aagcgtcctt tcaatcaaag acccatcaca tgagattctt
    31081 gtttctgctg ttcatcgggc tgagtccctg gcttgcccag ggccagaaat tcggccatat
    31141 caattccgcc gagatcctca gcaaggtgcc agaaattgcc caggcggata ccatcctaag
    31201 gcaataccag gataccctgg tggccgctgg agattccctg gtcaggattt ttcaaaaaca
    31261 cgttgcggaa taccaggcag agtcccagaa aggaaacctg gcgcccattg ccgcgcaaaa
    31321 aaaggaggaa gaccttgcca aagagcagga agtgctgagg aattatgaaa atgagatcaa
    31381 acaacgcctc gtcctgcgta gggaagccct ctataagccg atcctggaca aaatcgatca
    31441 ggtcgtgacc cgtattggaa aagaaaataa atacaccatg atctttgact cttcccaacc
    31501 cgggtacctg tacctggcgg agagcgataa tctcgggccc caggtactga aagaactggg
    31561 cctccagtag ccgtgcagga taaaataaac cgggggaagt atttcttccc accggttcta
    31621 ttggggttta tttaaaacga tggtcctttg gcccggatca ggtttccatt acattttgac
    31681 cagggactga tctccgtcca tacctctgaa taagatgacc tttccgtctt taaacttgaa
    31741 atgatgggtg taatgggatt catatctttt tcccgaaggg aggtgggtgt attttgcttt
    31801 cacccggcag tacacattgt ccccgctggc tacaaaatca taaggttcga atacctcgta
    31861 tttgaatttt tcattcagtt ctttgaagaa ttgggcgact tcctttttcc ctttgtacca
    31921 acgggtgccg taatcaaaaa cccggtcatc aatctcaaac atcaggtcgg gatccgaacc
    31981 ggagagaatg ccattgacat ccccgcttcc gaatttttta taaatatcca tgaccgcctg
    32041 ggcattggaa ttgcctaaaa aaccatatcc aatctgttcg aaaatgaggt tggggttgct
    32101 cacggcgtga gacagggcct ttccggcgcc atccatttcg atgacatcga catcggtgaa
    32161 ttccactttc ttgccggtcg gcggaagcat gcccatcacg gaaccggtat tggtaccggt
    32221 cagtttgaca atggtgtaat acttgttgcc ctcgttcacc gtctggatta cggtaaactt
    32281 catgtccgga aaggccttca tgaaggcttc gtaagcaggt tgtatgtttt ccctcccttt
    32341 gaccggcatg ggcgccgccg tataatccgt aaattcgggg gaaaccattg taaagaagag
    32401 gtcaaaattg cgcatattca atgcctcata ggccgcccgg ataagggctt tgttttgctc
    32461 catgactttt ggtttctggg cggacaaagc agaagcggtg aagaccagga accacagcgt
    32521 ggtttttgcg attgtaattt tcatgtgggg tttagttttt gttgaataat ttccacaaaa
    32581 ctaaattccg ggactgtccg gaatccagtc cagggattat tcaattattg ttcaaaaaag
    32641 gagaaaaacg gcggctcagc aggaagatat cttttgcacc cgttcgctgt ggcggccccc
    32701 ttcaaagcct gtttgaagga agatctccac cattcgaacc gcttcttccg ctgaaataaa
    32761 tcgcccggga agggcaatga tgttggcatc gttgtgcagg cgggcaagcc tggcgatatc
    32821 ctccgtccag cacagggcgg cgcggatgcc ctggtgtttg ttggcggtca tggccacccc
    32881 gttcccgctc ccgcaaacca ggattccaaa attggccgct ccctgttcaa cggcctctgc
    32941 caccgggtgg gcgaaatcag ggtaatccac ccgttccggg ctatacgtgc catagtccat
    33001 acaggcgata ttttgttcct gcaggctttt aaggatggcc tgttttactt caaatccggc
    33061 atgatccgct ccgagggcaa tggtcattgg tctggcgctt gtttatgcaa atatcggttc
    33121 tccgggctta ttatttataa tttaaggttc cccaaaacac cgcaacccgg tagaaggtcc
    33181 gcacctcgca agtaaaggac cttaatattt tgcaaactcg tcttagcttg cacgatcatt
    33241 gccaatcacc acatcatgaa acctgccata ttccttctga ctttgtgttt atgtgtcgcc
    33301 tgcaaaccgg aattgcctgc tgacctcatc ttggtgggcg gtaatattta caccggtgac
    33361 cggaaaaatc caaaggccag cgccctggcc gtccgggaag gcaagctgat ctacgtcgga
    33421 gatgaagccg gtgcgaaaaa attcgaaggg ccgtccacgg agcgggtcaa tgccggcgga
    33481 aaattcgtaa tgcccggctt catcgagggg catgggcatt ttgccggact cggaggcagt
    33541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tccccggaac aacttgaaaa
    33661 aatatggcac gacggcattg aactggccca ggaagaatgc ctgcgcaaag gagttacctc
    33721 gtttcaggat gcgggcagca gtttccagga aatggaatgg tataaagcgc tcgcagaaca
    33781 aaacaagctc aaaataaggc tatgggccat gctccggcaa aacagcaggg aattgacctc
    33841 tgaggcactg catacctttc cctggatcgg tctgggcaac gatcatttta ccatgcgggc
    33901 ggtgaaggcc gatgtggacg gtgccctggg ctcctatggg gcctggttgc ttgcgcctta
    33961 ttctgaccgt tcagaccatt tttacgggca gaataccacc gaagtcagcg aggtcgatgc
    34021 ggtagcccgg atttgtattg aacatggact tcagctttgt gtacacgcca tcggggacag
    34081 ggccaaccgc gaagtactgg acctgatgga aagacatttc gaagcgcatc ctgaaaataa
    34141 agacctgagg tggcggatcg aacacgccca gcatctggat acggccgata tacccaggtt
    34201 tgcggaaatg ggggtcatcg cttccatgca gggtatccat tgcaccagcg atgccccttt
    34261 tgtggtaaaa cgcctgggtg aattccgggc ccggacaggg gcttatgcct ggaaaagcct
    34321 cctgcggacc ggtgcggtag tcagcaatgg gaccgatacg ccggtcgagg atgtggatcc
    34381 gctcgcttcc ttttacgctt cggtgacccg gacgcgtgcg gacaatggca tgaggttttt
    34441 ctccgagcaa aaaatggacc gtgaagaagc ccttcattcc tatactctcg ctgcggctta
    34501 tgccgctttt gaggaccata ccaaaggatc gctgagcccc ggcaaatggg ccgattttat
    34561 tcttctgtcg gaggacctgg ttcgctgccc ggatgaagcc atcctgaaaa cggaagtcct
    34621 caaaacctgg gtgggtggca aattattata ctcaaaggaa taagaacatg taccgttgga
    34681 tctctctggc ctgcctgatc tgcacggcca acccaatcgg ggcgcagtct ttgctccggt
    34741 ttaatgaaaa ttccttttac aaacccccgg agaacccgat cctccgggcg gattccaatt
    34801 ttgtatttat ggatccggtc aaacagcgca tggttcgatg gcagcaggcc gatgtattca
    34861 accccgcggc catcgtccgg gacggcaagg tttatctgct gtaccgctgt gaagacaatc
    34921 cggtggccca cctgggcggc cggacctcca ggttaggact ggctgaaagc acagatggca
    34981 ttcattttac caaatatccc caaccggtgc tttatcccac ggacgatcct ttccgcgaat
    35041 acgatgatcc gggcggctgt gaggatcccc ggctggtaca aaccgaggac ggcctttacg
    35101 tagtggctta tacttcctgg aatcaaaaga tcgccaggtt atccatcgct ttttccaggg
    35161 atttgatcca ttgggagaaa aagggg
//
LOCUS       DAOQXT010000042        34504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1459_length_34504_cov_6.812738, whole genome shotgun sequence.
ACCESSION   DAOQXT010000042 DAOQXT010000000
VERSION     DAOQXT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 34504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34504
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1459_length_34504_cov_6.812738"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1078
                     /gene="bamD"
                     /locus_tag="PKM27_09200"
     CDS             263..1078
                     /gene="bamD"
                     /locus_tag="PKM27_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNR07479.1"
                     /translation="MFKNRTLFLFVTALLSLAACKSEFEKVRTSNDTDAMYKKAVEYF
                     EEKEYSKAQTLFELVVSSFRGRKEFEQLNYLFAYTYFHQRDYVAAASAFKNFANTFLY
                     SPYKEEMDYMYAYATYMQSPNFRLDQGPTVKAMDALQIFVNTYPNSERTKEATRLMDE
                     LRKKLEAKAFSQGQQYFDMGQYQAAITTFENVLKDFPDTREAENIRYTISKSGFLYAE
                     KSIFEKKEERYQEAIKYAETFISKYPRSKYRGEVQDFIQISKSRLNQIKNVGH"
     gene            1065..1433
                     /locus_tag="PKM27_09205"
     CDS             1065..1433
                     /locus_tag="PKM27_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNR07480.1"
                     /translation="MSDIKNKVQGIDPNVKARDIKAMSEKIGNLYEAISIISKRANQL
                     NMEVKRELQSKLEEFAVSSDTIEEVHENKEQIEISKFYEKLPNPALLATEEFLHNRLS
                     FRYGEVQAPEEGLSLPEGKA"
     gene            1454..2668
                     /gene="coaBC"
                     /locus_tag="PKM27_09210"
     CDS             1454..2668
                     /gene="coaBC"
                     /locus_tag="PKM27_09210"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNR07481.1"
                     /translation="MDLAGKKILLGVSGSIAAYKAVVLLRSLQKAGASVRVIMTRGAS
                     DFVVPLTFEAISHHPVSIENSTNQSWNNHIELSLWADLFVIAPASANLMARMAAGMAD
                     DMVTACYLASRAPVCLAPSMDVDMWHHPATKHNIEILRSRSVQIIPVGYGALASGLTG
                     EGRMAEPEEILEFIGRILSPEAQTLAGKKAMVTAGPTKEAIDPVRYISNASTGKMGIA
                     IADALASRGAEVQLILGPVALKPGHPKVNIHPVTTAEEMYQAALQYHERSEISVFCAA
                     VADFRPAEVAREKIKKAGSGLNLKLVKNPDIAFELGRKKKVHQVHIGFALESGAGEAQ
                     AREKLEAKNFDLIVLNSLQDAGAGFGTDTNKITFFLKGNKSRKFGLKSKEAVAGDIVD
                     AIESLLKKLKKP"
     gene            2665..3561
                     /locus_tag="PKM27_09215"
     CDS             2665..3561
                     /locus_tag="PKM27_09215"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNR07482.1"
                     /translation="MKKYLMIFWGLALAVSLSAQELIAQFKVSTPKLQNTDPAVFRDL
                     EEDARQFINTRTWTNDKFANQERIKCNFLLTIKEELGSNTFKADLTVAASRPVFGSNY
                     ETTLINYIDREITFNYEPNRPLNYVQNSYTDQFTAILAFYINIILGLDYESFSLNGGN
                     DYFLICQNIVNQIPANVNASGWKAGDGNRNRYWLIESILSPRLRPMRQAWYQYHRLGL
                     DLMHENATEGRKAIQRAMESIDLAKRNYPVGNWIQLFVDAKTSELVEIFKKADKPQQD
                     LIFNILNRLEPSSASKYQAIYQ"
     gene            3568..4140
                     /locus_tag="PKM27_09220"
     CDS             3568..4140
                     /locus_tag="PKM27_09220"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNR07483.1"
                     /translation="MKSLAIFASGRGSNAQNIIRYFRQSKSIRIRLIVSSHEKAGVLD
                     IARNEGIPSLVLDKKEYYGSGESLVKSLTDYQIDGIVLAGFLWLVPTYLIENYPHAII
                     NIHPALLPKYGGKGMYGMHVHEAVWKNKENHTGITIHEVNERYDEGKTLFQATCPLSP
                     QDLPEEIARKVHELEYAHYPRVIEEYFNSQ"
     gene            4599..7388
                     /gene="ppdK"
                     /locus_tag="PKM27_09225"
     CDS             4599..7388
                     /gene="ppdK"
                     /locus_tag="PKM27_09225"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNR07484.1"
                     /translation="MKKSTSKKPAGKTPKYVYFFGGGRADGNESMKNLLGGKGANLAE
                     MAGHPALRLPVPPGFTVTTEVCTYYYDHKKSYPPTLDAEVKNSIIKMEKLTGKVFGDL
                     KNPLLLSIRSGARRSMPGMMETVLNVGLTEKTIQGLIDQSGGNERFAYDAYRRLIMMY
                     ADVVMEKGAGVEVKNGKGIRKKLDEKLENLRNSKNYPSDDFIPAADLKELVKDFKTTI
                     KASLGKAFPEDPWEQLWGSIGAVFSSWNGKRAVEYRRIEKIPDAWGTAVNVQSMVFGN
                     MGDDSATGVAFTRNPGNGENRFYGEYLVNAQGEDVVAGIRTPAPINEYSKNDQSKHLP
                     SLEKRMPKLYKELFGYQKRLEQHYRDMQDIEFTIEKGRLYMLQCRVGKRNGIAAVRMA
                     TEMHGQKLIDLETAIRRVSPNQLVELLLPMFDPKVELVTKPIAKGLPAGPGGAVGKVV
                     FSSDEAVAWSQKGEKVILVREDTSPEDVDGMHKSQAILTTKGGMTSHAALVARGWGRC
                     CIVGCGEIEIHHESKTFKTKSGITVREGDWISLNGTKGLVYHGQLPLIATDLGKNKSY
                     KDLMRLVDKVKVMGVRTNADTPKDAAQGLAFGAEGIGLFRTEHMFYGEGSDRPLFLLR
                     KMIMSKNEPERRTAINELFEFVKKDTKATMAVMAGHPVTIRLLDPPLHEFVPHDSEKL
                     HQLSKELGISMGILRRRVLALHENNPMLGHRGVRLGISYPEITEMQVRAILEGAAELI
                     KQGKKVFPEIMVPVTCSANELIHQKKIVDAIYKEVCAKKKINKIPYSYGTMIEIPRAA
                     LKAGHMAEVADFFSFGTNDLTQMSFGFSRDDVGGFLPVYLDQGILHDDPFQTIDQAGV
                     GELIKFGTERGRAVKPGLKVGICGEHGGDPESVKFCHRIGMTYVSCSPFRVPIARLAA
                     AQAALENPVKPVRKK"
     gene            8073..8885
                     /locus_tag="PKM27_09230"
     CDS             8073..8885
                     /locus_tag="PKM27_09230"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNR07485.1"
                     /translation="MNCLFCKQNSDNSKSVEHIIPESLGNKDQVLPKGLVCDSCNQYF
                     ANKVEKPLLEQPYFRNVRHRARIESKKGRVPIENAFIISPELAKADIIIDRKEDKTII
                     VMEDEEKARRIFSSPKGSMIIPAFDKPEDNNKILSRFLAKVAVEGLLHWLLKEDGWID
                     EVMTNSGLDEIKKYARYGQGVEFWPYHQRRIYSEETRFNHPLISIQDYEVLHEFKILP
                     TKESHYYFVIAIMGIEYAISLGGPGTDSYEQWLKENNYASILDDPNEKKSSR"
     gene            9444..10274
                     /locus_tag="PKM27_09235"
     CDS             9444..10274
                     /locus_tag="PKM27_09235"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNR07486.1"
                     /translation="MDKIRLQKLLFLFSLKQTKAEYDFVPYKYGCYSYSANADLTTMV
                     SKGLLAESQKHFDKLDRTDYLKLVKPDDLKRLQQVKAEYGKMSSKVLMKHTYINYPFY
                     ATKSEVAGDILTETELQKVNAAKPRNNETVLFTIGYEGISLEEYLVRLLKKDVKVLVD
                     VRNNPLSMKYGFSKSQLKRYCENLGIKYVHIPEVGIQSEQRQELKTQTDYDKLFSVYR
                     KNNLTKTVDSQTQILNLLKEYKRIALTCFEANICQCHRKHLAEAIETLPGFQYKVEHI
                     "
     gene            10278..11105
                     /locus_tag="PKM27_09240"
     CDS             10278..11105
                     /locus_tag="PKM27_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07487.1"
                     /translation="MARKKVLISVTTYPLPSRSYDELVCTAGLLETGEWIRIYPIPLS
                     FLMGQRHSGKIESFKYTWIELDLNKRTDDFRPESHSPQNYDFRDIVIYDRVGTESNWL
                     KRKKFCLQNVYTNLSQLIKDSEAPTNMSLATFKPTEIVGFEIENDTREWKDEWVEIRK
                     QGDLFAPEKPPETLIPKLPYKFFYRFKDVAGKESRLMIEDWEIGALYWNCLKATEGNE
                     AVALEKVREKYEGEFLAEKDIYLFLGTTKEWHLRRSKNPFVIIGVFYPKKESQMKLF"
     gene            11495..12205
                     /locus_tag="PKM27_09245"
     CDS             11495..12205
                     /locus_tag="PKM27_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR07488.1"
                     /translation="MSDSYHVTNRDFKGYTKKELDEMASDPNSLLTEWSKKRALKKTI
                     KKARKEMSNSIKLTSLMDKQMVDEIEQLKKNMFSEDWELVKSSADKLGEIGGNEITDF
                     LISLLFLDDSGIRDRAALALEQIGDNKAVEPLFKSIFKNHNYNGTMVFALESLDCSKH
                     LKDIFKILFFEAYESKMSAMAILDTQIFEFTSQDLIEIANMWEDCKLHPEKCPEIEDD
                     DVRKEMQESVNGFLEYLK"
     gene            12420..12791
                     /locus_tag="PKM27_09250"
     CDS             12420..12791
                     /locus_tag="PKM27_09250"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR07489.1"
                     /translation="MTEDFLQSFADAFNAHDLEAILSHMTADCVFEASAGPDADGQKY
                     VGQKQVKKAFGDVFDNFPDAQWNHPRHFISGDRGFSEWTFTGTKKDGTRVEVTGCDLF
                     TFRDGKIAIKNSYRKNRLETR"
     gene            12853..13239
                     /locus_tag="PKM27_09255"
     CDS             12853..13239
                     /locus_tag="PKM27_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07490.1"
                     /translation="MKINVMFMIAAVYGFVVGLPVIFAPEATASMGGIQIPDGMSMSL
                     RFLGVAELGLGTIAWLVRNAEASKTRDGVALGFTIYFGLHSLASWYGQFTDPSTTMHW
                     VMASLQALIAVGFFLAGRANRSKSGN"
     gene            13692..14135
                     /locus_tag="PKM27_09260"
     CDS             13692..14135
                     /locus_tag="PKM27_09260"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR07491.1"
                     /translation="MVGICHALLLISCTAPAGSSGLSATDKEYIQSLTQTVEENWNKG
                     NREPYVNRFTSDAFYMAPNMESLVGKDAIKSFAASFPELKLEFTIGEIEGSSEFAYVR
                     GGYLLKTPQDSLFDKGKFLSIWEKTPEKTWLLSHDMFSSDLPPTK"
     gene            14385..15032
                     /locus_tag="PKM27_09265"
     CDS             14385..15032
                     /locus_tag="PKM27_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNR07492.1"
                     /translation="MENLNELMLKAAFKSEWEEVEKWVSRGADINYRDPHGNFALFTA
                     AWEGHLPAMDLCYGLGAQISFEDANLLCNAAYNGKTDSVNWLLTKGENADFSFRDTGE
                     NALHYTLSKTSEMDERTEIVKILIEAGTQVNKKTMAGQPTLCFMRDAWLKGETPLHRA
                     AAYADAAIIKMLLDAGADPSVKDANGDTPMSWGSWHLREASVLRLLQYEGVPRIH"
     gene            15150..16799
                     /locus_tag="PKM27_09270"
     CDS             15150..16799
                     /locus_tag="PKM27_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07493.1"
                     /translation="MKFIPILFFLSTCLVGSWAQNEAPREKVVITSKKQKTRVAINAK
                     GALEVNVSPRNILKFKTTGWVRYSDFGARGDGKADDMDAIAATHAFANQQGLMVKADE
                     GATYYIGGKERTAIIRTDTDWGTASFIIDDTEVQNRNAPVFMVSSSQQSFKLEGISSL
                     KRNQNNIDAALPGSCLISVTNAHVKHYIRFGLNQDNGSPQTDIFVVDKNGKVDMDGPV
                     IWDFDQITDITALPMDEKTLTIHGGRFTTIANQAESKYTYYSRNLAIRRSNVVVDGLE
                     HRITGEGDQGAPYGGFIHISDCAYVTVQNTILTGHKTYRTIGAAGEPVSMGSYDILVN
                     RALHVSFKNCSQTNDIDDRTYWGIMGSNYCKNLLYDRCTLSRFDAHKGVANATIRNST
                     LGHMGINAIGSGILTVENCTIRGRSIVNLRSDYGSTWQGEIFIRNCLFVPEGGRPSRA
                     VLISGSYSGQHDFGYTCYMPERITIENLHIDDSSHPEDYRGPALFANFNPQMTDDSYR
                     ESFPYVKTREVILRKVTTASGKTLRLSDHPFMFKEVQVSSE"
     gene            complement(17152..17520)
                     /locus_tag="PKM27_09275"
     CDS             complement(17152..17520)
                     /locus_tag="PKM27_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07494.1"
                     /translation="MIKYVVILIGLMFPVACSQSVVESAENDLITKKGLLLNQLAVDG
                     CSWHFAVAMGDEWGQYLASDQSNDLVYPLIEKAMTLTGTYSIGVEITFRLTGRKKEVE
                     CGWGKHLSMDEIEVVQIREV"
     gene            17683..19311
                     /locus_tag="PKM27_09280"
     CDS             17683..19311
                     /locus_tag="PKM27_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNR07495.1"
                     /translation="MKHFFLTFSCLLMISLHAQDNNSFPVRTKIANGIIEGNYDTKTG
                     LQQYFGIPFAKPPVGTLRWKAPQPLVNWTGVKQTKKFGPRPVQGIVFGDMMSRSDGLS
                     EDCLYLNVWTPAKRNTKGLPVLVYFYGGGFVAGDASEPRYDGASMAQKGMVVVTTNYR
                     LNIFGFLAHPELSAESPYKASGNYGLLDQHAALAWVQKNIAAFGGDPKKVTIAGESAG
                     SISVSAQMVSPLSRNLIAGAIGESGAGIFPTLAPVPLAQAEKEGADFAKNAGYPSLAS
                     LRALSTREVYEIYQEARRFGFPTVIDGYFYPKTIPEIFRAGEQAKIPLLLGWNSAEIP
                     GGAFMQGSPYTSENFITKVKAAFPDDADEILKLYPHSTAKEVEYSATALASDRFIAYS
                     TWKWFDLHRKHSSQPVYRYLYSKLRPPLVDKSLSPGLAGGTTRSDQPAPPPPPAIGAP
                     HACEIEYCMGNLHLVKEYAWTSDDYKVSETMQNYFANFILTGNPNGAKLPEWPAASPN
                     DPMPPVMILDTESRSVPAKDDGRYLLLDKFYKNQ"
     gene            19392..22100
                     /locus_tag="PKM27_09285"
     CDS             19392..22100
                     /locus_tag="PKM27_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNR07496.1"
                     /translation="MFPSFLNRHPSLAVKNAFIYLILLIFSSSLIGYSIYRLSAALIL
                     SSSKTQIRNEVRDMAARMTGHFDYIGRDVRFLQNNPLIQEYLKAPESQRKNLADKLGD
                     EFKALLESRPAYFQVRFIGVANNGKELVRVDRDGRQGKILAEAELQEKGDRSYFTETI
                     RQAENSIYFSEIDLNREHGKIDFPYIPTLRIAGPVYKEGRAFGIVIINTDLDALFGDL
                     KKATGLYEDLYIFNNSGYFLVHPDEAECFGFEFGKPPNLKHWFADLNALDPVFTRLPE
                     TDSMVSREKEVTEFYTLLHPKTDYKLFIALSAPESAILYPFKSWKKNMMALTFAITFL
                     FLVIAFLWMRRQSRELKKITRSMVKFSEDYSESKLLIRQTDEIGELAKAFSNMAGTIR
                     NNVKELEMSREAAVKANKSKEEFLENMSHEIRNPLQSILGMTNILETNHPRPDQETVI
                     KTIRFSSRQLLSLVNDILDFSKLRIGQIRLQAQDTDLADLLSEITRSHLFLAQSRNIS
                     LESHVPPGLKGTLFRVDPLRLSQILNNLLVNAIKFTNPGGKVVLDVEANAGDQAMSLR
                     FTVTDNGTGIPPDELDKIKKRFYSRDATELNTHLSGAGLGLPIVIQLLELFHSELEVE
                     SEIQKGSRFSFSLELPVTPPAATSLAGHSSNRGLPPGITQVLCMDDDPQIIYFFRQVF
                     RTLEKDLVCIGEPGELDTLPTGRRFDLIIADFMMSSGNMIRSLPRLARLKSEHGVLII
                     LSGIDVQSRLGESGMQGVDLVLQKPISSSALLAQIQKAWDNAHSPPPWMEDFFNDYDH
                     EEALISHALKILIEEWKTMQGQLIQCLNARDKEAFDQVFHKIITTLRRFRLHHLLKVL
                     EQIQEQFSVSPAEARENTIILSSLMDKTRQEFEYQLHHLPSILTTH"
     gene            complement(22087..22782)
                     /locus_tag="PKM27_09290"
     CDS             complement(22087..22782)
                     /locus_tag="PKM27_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR07497.1"
                     /translation="MGLKCLIIDDDPLIGDLLKHFCTKSGLVDYCILSATATDGLRLM
                     AGGEIDLVFLDYNLPDMNGQNFLELKKSRLPVIMVTSDEAFAARSYNYEDVVDYLVKP
                     LSYDRFLKAMQRVTGRQSAIPESPGPVTDVFFAKDGNKNVRIKLSEVLFIKSEDNYVA
                     FVFPESQTLSLFTLKDLEAKLPRNFKRVHRSYIVNMDRIDYLTPEEIVLGDKKVPIGE
                     KYRSDLEASLNAL"
     gene            23187..24092
                     /locus_tag="PKM27_09295"
     CDS             23187..24092
                     /locus_tag="PKM27_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNR07498.1"
                     /translation="MTRIFAAVLLFRVLLFPAFLSAQQPNDAIYMDKNAICIVAAYGH
                     NSWDTYWENTLKRQNLNIGTNTTQSFALMAAAGITDKLNLLVGLPYVSTRNSAGNLLG
                     QKGLQDFSAALKYKLFNNEGLSLHGTLGGSIPVSNYVPDFLPMSIGLHSATASARLVF
                     NYLHKSGIYFTAHGAYTARSNVRTDRDSYQANDKIYNSDELAIPNATDGGINIGYLKN
                     SLQATLFLERFDCVGGDHIRRNDAPYPTNDMEATNIGAYVKFQPGHFGVNARYAYTTS
                     GRNVGQTTSLMAGILYQFSIKKSSN"
     gene            24126..25661
                     /locus_tag="PKM27_09300"
     CDS             24126..25661
                     /locus_tag="PKM27_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNR07499.1"
                     /translation="MKMKLFPVIALVGAFIVACDKDIQNPLLEPYAVSSLDENAGQWK
                     PYILSSVSDIVVPEPKPVTDPAYLKELDSLKNYIAPRVTPENRAAVEHWGAGAVYRWN
                     EIARELSARYNLPPASNAEGKYPVPDAANPLKDPKFPFANPPYAARALAYLSVAQFDA
                     LVTAWNYKYKFNRKAPYKIDSGIKPLLPASDLPSYPSEAAVVAAASYTVLIAMFPGEV
                     PYLDGKLAENKNAMIWAAMSTPSDITAGDALGKAVAARVMAKAKTDGMSAANNQSRTP
                     AMIETAKSIGIIEPWKSQESPLRPTMLPTYGEVATWNFSKETMIAMRPPKPWIVGTPE
                     FEKDLKELQGIQKNQTREQARIANFWADGAGSYTPPAHWHRIGAEAGKDAKYSEVRMA
                     RTLALLGTAEMDAGIACWDAKHYYFTPRPQQFGVKTSVGLPNFPSYTSGHSTFSSAGA
                     TVLSYIFPERKNEFEAMALEASNSRIYGLIHYRVDCEMGVAHGKTIGGYAVQRGQTDG
                     SGL"
     gene            25689..26768
                     /locus_tag="PKM27_09305"
     CDS             25689..26768
                     /locus_tag="PKM27_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07500.1"
                     /translation="MKKYSTILTLSLLSVLVGHDALQAQGCIAVRHMSCATGGINNTN
                     TAMNKGQFQFVAGYRYLHSYKHFVGTVEQTHRVEQNTQVINNAHSFDFGLTFALNDRF
                     SFALNVPYNDNARSSLYEHYGNAESANPNRSRFSTYSSGIGDIRLTATYWIFHPQKSM
                     NGNVAVGLGVKAPTGNPNAKDNFHKLDKNGKEYLQYKTVDQSMQVGDGSWGFNVEMQG
                     YQALFNNTSLYYNGFYLFSPRNINPLTGFSVADQYAARLGLNYSILPKQGIAVSLGGR
                     IEGLPAIDLIGDSEGFRRPGYIISVEPGIFWMTGRHTFAFNLPIAVERNRIKSWQDQQ
                     DPLGLRHGDAAFADNFISATYSYRF"
     gene            26989..27579
                     /locus_tag="PKM27_09310"
     CDS             26989..27579
                     /locus_tag="PKM27_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762506.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HNR07501.1"
                     /translation="MPHIPVEEHLPGITGLLEYRKDTAAPIRALTQFLLRGSSSLSAG
                     ERELIASLVSSRNQCVFCTQAHTTAADILLGEAESCRQVIENYPQAPVREKLKALLHI
                     AALVQVSGKKVDSAAIAQARQAGASDQEIHDTVLLAGLFCLYNKYVDGLATGLPSHPE
                     YFKQLGERITTIGYVRLPGGYDHLKKKSGSESSKDL"
     gene            27605..30364
                     /locus_tag="PKM27_09315"
     CDS             27605..30364
                     /locus_tag="PKM27_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR07502.1"
                     /translation="MKNLLLFTLLLITNVLIGQNITGKVVDAQTKKGLEGVAVAIKGS
                     AEGTYTDAQGMFSIRTSESLPLTLVTSLVGYESMEINVSQPGPVEIVMRSTIEELNQI
                     VVSASRIEERLLRSPVAIEKMDLRSVQQTPSANFYDALVNLKSLDLVTSSLTFKQINT
                     RGFNHTGNGRFLQLVDGMDNQSAGLGFAMGNLFGPHDLDVSSVELIPGAASALYGPVA
                     FNGMLHIQTKSPFDFQGLSVQTKAGLNHISDGTGEGAKPFYDLGIRYAKAWNNKFALK
                     LNASYLAGTDWYANDYTDIDPNTPEGSRGPNNPGRNALNIYGDEVAQTIEGIGRVSRT
                     GYEEKDLANYDVSSLKFNGALHYRISDQLEALYQGNFNRGVAQYTGSSRFVINGFNLV
                     QHRLELKGSHFYLKAYSTRENSQDSYNTRSLGQLINRSWVKDLSGATVSPANADATWF
                     QRYAAAYKGQVNGTESNNHLAARAFADNGRFLPGTSAFESARDRLINTQGLQGAGILS
                     KCGIKHAEGMYDFSHLLANAFELQVGANYRIYDLNTEGTLFDDKNKNLTNDEIGAFVQ
                     LSKNLLKDRLRLIVSGRYDKNENFDGRLTPRASAVYSFNGNNHFRISYQTGFRNPTIG
                     DQYIKLNVGPIIILGGAPVNAAGLNAYENSFTAASVGAFGSGFGAQIAQGVPFPQALA
                     NNKDKLVKSNVPYIQPERVSSIDLGYKFLLNQRLLIDLGYYNSRYRDFLINTVVIRPD
                     HDVLANGSINPAAAADILNGKIQAFQLYTNAADEVTVQGLTSGLTYFLPKNYRLMGNV
                     TWSDFNLLNADPNNIPAFNTPEWKTNLTFSNPALTSKLGFSVAWHWQNAFDWYGTFTG
                     LIPGRVDAYSLLDAQVSYKLPSLKTTVKLGASNITNQYIVQAYGSPAVGGIYYLSLTF
                     DELMR"
     gene            30372..31604
                     /locus_tag="PKM27_09320"
     CDS             30372..31604
                     /locus_tag="PKM27_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR07503.1"
                     /translation="MKKISAQHQWTLGLSLIAYLFAGTVSTLVSVYLPVIVNDLKGYR
                     VPEQELGTLGAFVNAFFLYGWMAGGILMGLLGDRFGRKKILALSTALYGAATLLIVFV
                     HDWRLMMFYRILAGLGVGGTLVLSTVMISETWPEKSRAVIQGILSVSFPVGIVATGGL
                     NILFEIWRRAFWLGLIPILVATLMILFLKEPATWNRSASQGTKLKALLHPPVRYNLIS
                     GILIFGSVLIGLWGLFSWMPTWVQSILPEGYAGTRERGLTMMLLGIGGITGGLFSGFL
                     VRVLGARKTLSLAFTGLTAVSLILFLTNPRFSEIIYPETVLLALFFGISQGSLSHYIP
                     ELFPSPIRAAATGLCFNIGRFFTATAVFFVGSLVTLFGGFGNALLVFSITFVIAFLAV
                     YFTRAEPERQLPIPQTVP"
     gene            31658..32203
                     /locus_tag="PKM27_09325"
     CDS             31658..32203
                     /locus_tag="PKM27_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762503.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HNR07504.1"
                     /translation="MPHIPLPEGIPGIRSLALQYPESGKYLYELVQILLRHESPLSEA
                     ERELIAAYVSYLNECEFCCKSHAAASRYLFADASGTVDMVLHDYRSAPISDKLKSLLS
                     IAGKVQADARTVTEEDVALARSFGAVDRDIHDTVLIAATFCMFNRYVDGLATLTPEDS
                     QVYAEMGERMATKGYVVPEQK"
     gene            32459..32734
                     /locus_tag="PKM27_09330"
     CDS             32459..32734
                     /locus_tag="PKM27_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07505.1"
                     /translation="MKEKAGGSPRLEALLVLARQVTWNGKKVTSEQITKAKALGCTDQ
                     EVHDTILIASMFCMFNRYIDGLGLTSRDTPESLKTRAQHLAEYGYGT"
     gene            32770..33321
                     /locus_tag="PKM27_09335"
     CDS             32770..33321
                     /locus_tag="PKM27_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNR07506.1"
                     /translation="MSFIDLKNDLPGIRSLMAYSPHTAKPLNELAEVLLRDEHNTLSR
                     GERELIGAYVSWLNQCFFCQQVHEALAGHYLACPIGELDQIFDNLGASALPAKIKALL
                     FIAGQVQTGGKSVSQDQVDAARHAGASDREIHDTILIAAAFCMFNRYVDGLGTWAPAD
                     RGYYVNRAPQRAIEGYIDFDLYK"
     gene            33328..34464
                     /locus_tag="PKM27_09340"
     CDS             33328..34464
                     /locus_tag="PKM27_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HNR07507.1"
                     /translation="MNKLFWLGLAGWILFESSNVYFIMPMPGSQQMNSLDLAYFLYTW
                     RWLFRALFAGLILLGFRHVHWKKKWHMALPFAALLGVIYLFNFNMAADHMFYQPSRVI
                     FKNASQNEVDSNRLVIGLTHQGEAKAYPVRFMGYHHQLLDTVGGKAVLVTYCTVCRTG
                     RVYEPRVNGKPETFRLVGMDHFNAMLEDKTTKSWWRQVNGEAVAGKLKGSMLPEVFSI
                     QCRLEEWIKLHPHTLIMQADSTYIDSYPDNYKFESGKSKSKLTGTDSLSWNRKSWVVG
                     IRTPQASKAYDWNRLKSEWIIHDEVRGTPILLVLASDTASFFAFERPGPDARFTLVKD
                     TLYLGQHRFDLKGRGLDTTLHLKPLQAYQEFWHSWETFNPGTLR"
BASE COUNT         9292 a         8962 c         8161 g         8089 t
ORIGIN      
        1 acggtcacca ggttggtctt catcacggta tacaccgggg cgtgcaaatc catactgctt
       61 aattttctgt aaacttaaaa aggattgttc aaattgctcc gggaaaacgg gaaggcggga
      121 ccatggaaat gcaataatcc cggcacgacc atccatccaa ggaaagaatc aagccgggca
      181 ccatcctaat cctcagaata tcaattacct gcagggtaaa tatttcagcc ggataacaca
      241 aaaaacctta aatttgcccc ccatgttcaa aaatcggact ttgtttttgt tcgttacggc
      301 cctgcttagc ctggctgcct gcaagagcga gtttgagaaa gtcaggacca gtaacgacac
      361 cgacgccatg tacaaaaagg cggtggagta ttttgaagaa aaggagtatt ccaaggccca
      421 gacccttttt gaactggtag tcagcagctt ccgcggacgc aaggagtttg agcaactcaa
      481 ctacctcttt gcatatacct attttcatca gcgggattat gtcgccgcag cctccgcctt
      541 caagaacttt gccaacactt ttctatacag tccttacaag gaggagatgg actatatgta
      601 cgcctatgcc acctatatgc agtcccccaa tttccggctc gaccaggggc ctacggtgaa
      661 agcgatggat gctttgcaga tctttgtcaa cacctatccc aacagcgaaa ggaccaagga
      721 agccactcgc ctgatggacg aactgagaaa aaaactggaa gcgaaggcct tcagccaggg
      781 tcagcaatat tttgacatgg gccagtacca ggcagccatt accacttttg aaaacgtgct
      841 gaaagatttc ccggacaccc gcgaagcgga aaatatccgt tacaccatca gcaaatccgg
      901 ttttttatat gctgaaaaaa gcatctttga aaaaaaggaa gaacggtacc aggaggccat
      961 caaatatgcg gagaccttca tttccaaata ccccaggagc aaataccggg gggaagtcca
     1021 ggattttata caaatttcga aaagcagatt aaatcaaata aaaaatgtcg gacattaaaa
     1081 acaaagtgca gggcatcgat cccaatgtaa aggcccgtga catcaaggcc atgagtgaaa
     1141 aaatcggcaa cctctacgag gcgatctcca tcatttccaa aagggcaaac cagctcaaca
     1201 tggaagttaa aagggaattg cagtccaaac tcgaggagtt tgcggtcagc tcagacacca
     1261 tcgaggaagt acacgaaaac aaggagcaga tcgaaatctc caaattttat gaaaaacttc
     1321 ccaatcccgc cctgctggct actgaagaat tcctccacaa ccgtctgtcc tttcgttacg
     1381 gggaagttca ggctccggaa gaaggccttt ctcttcccga aggcaaggct taatcccgcc
     1441 atttcccgcc aggatggacc tcgccggtaa aaaaattcta ttgggagtca gcggcagcat
     1501 cgcagcttac aaagcggtgg tccttctccg ttccctccag aaagccggcg cttcggtccg
     1561 ggtgatcatg acccgtggag ccagcgattt tgttgtcccg ctcacctttg aggccatcag
     1621 ccaccacccg gtctccatcg aaaacagcac caatcaaagt tggaacaacc atatcgagct
     1681 cagcctctgg gccgatctgt ttgtgattgc gcccgcatca gccaacctca tggcccgcat
     1741 ggccgccggc atggccgacg atatggtgac cgcctgttac ctggccagcc gggccccggt
     1801 atgtctggct ccttccatgg atgtggacat gtggcaccat cccgccacca aacacaatat
     1861 tgaaatcctc cggtcccgct ccgtgcagat catccccgtg ggttatggcg ccctggccag
     1921 cggactcacc ggggaaggaa ggatggctga gcccgaagag atccttgaat tcatcggccg
     1981 gatcctgtcc ccagaggctc agacccttgc gggaaaaaag gccatggtca cggcgggccc
     2041 aacaaaggaa gccatcgatc ccgtcaggta tatcagcaat gccagcaccg gtaaaatggg
     2101 catcgccata gccgatgccc ttgcctcccg gggagccgaa gtgcaattga tcctgggccc
     2161 tgtcgccctc aaacccgggc atcccaaggt aaacatccat cccgtgacca cggcggaaga
     2221 aatgtaccag gcggcgctcc aataccacga acgaagcgag atcagcgtct tctgcgcagc
     2281 cgtagctgat ttccgtccgg cggaagtggc ccgggaaaaa ataaaaaagg ccggatcggg
     2341 gctaaacctg aaactggtta aaaatccaga catcgcgttc gaactgggaa ggaagaaaaa
     2401 agtccaccag gtccatatcg gttttgctct tgaaagcggg gccggggaag cccaggcacg
     2461 cgaaaaactg gaagccaaaa atttcgatct gatcgtgctc aattccttgc aggacgcagg
     2521 ggccggtttt ggaaccgata ccaacaagat caccttcttt cttaaaggca ataaaagccg
     2581 gaaattcggg ttaaaatcaa aagaggccgt ggccggggac atcgtcgatg ccatcgagtc
     2641 gcttttaaaa aaattaaaaa aaccatgaag aaatacctga tgatcttttg gggtctggca
     2701 cttgccgtga gcctctccgc acaggaattg attgcccagt tcaaagtatc cacccccaag
     2761 ctgcagaaca cggaccccgc cgtattccgc gacctggaag aagatgccag gcagttcatc
     2821 aatacgcgga cctggaccaa cgacaaattt gcaaaccagg aacgcatcaa atgcaatttc
     2881 ctgctcacca taaaggagga actgggctcc aataccttta aggccgacct caccgtggcc
     2941 gcctcccgtc ctgtttttgg cagcaattac gaaacgacgc tcatcaacta catcgaccgg
     3001 gagatcacct tcaactatga acccaaccga cccttgaatt atgtgcaaaa ctcctacacc
     3061 gaccagttca cggccatcct ggccttttat atcaatatca tcctgggact ggattatgaa
     3121 agtttttccc tgaacggggg aaacgattat ttcctgatct gccagaatat tgtaaaccag
     3181 atcccggcga atgtcaatgc ttccggctgg aaagccggcg acggcaaccg caaccggtac
     3241 tggctgatcg agtccatcct cagcccgcgc ctgcggccca tgcgccaggc ctggtaccag
     3301 taccaccggc tcggactgga cctgatgcat gaaaatgcaa ccgaaggccg caaggccatt
     3361 cagcgggcca tggaatccat tgaccttgcc aaaaggaact atccggtcgg caactggatc
     3421 cagctttttg tcgatgccaa gaccagcgaa ctggtggaga tcttcaaaaa ggcggacaaa
     3481 ccccagcagg acctgatctt caatatcctc aaccgcctcg aaccttcctc ggcttccaaa
     3541 taccaggcca tctaccagta aagtccggtg aaatcccttg ctattttcgc ctccgggcgc
     3601 ggcagcaatg cccagaacat catccggtat ttccggcaat ccaaaagcat ccgcatccgc
     3661 ctgatcgtgt ccagtcatga gaaagcagga gtgcttgaca ttgccaggaa cgaaggcatc
     3721 ccctccctgg tccttgataa aaaagaatat tacggttccg gcgaaagtct tgtaaaaagc
     3781 ctgactgatt atcaaatcga cggcattgtc ctggcgggtt tcctctggct ggtccccacc
     3841 tatctgatag agaactaccc tcacgccatc atcaatatcc atcccgccct gctccccaaa
     3901 tacggaggca agggcatgta cggcatgcat gtgcatgaag ccgtctggaa aaacaaggaa
     3961 aatcacaccg gcatcaccat ccacgaagtg aatgagcggt atgatgaagg gaagaccctg
     4021 ttccaggcga cctgccccct ctcgccccag gacctgcccg aagagatcgc ccgaaaagtc
     4081 catgaactgg aatatgcgca ttatcccagg gtgattgagg aatatttcaa tagtcaatag
     4141 tcaatagtca atagtcaata gtcaatagtc agtcggctca caactcgatt ctggcatctc
     4201 gcttctcact ctagtgttcg gaaggagctt agctccgacc ttcccaataa taatagtaaa
     4261 ttaaaaatgt ataccagccc cagccaagtc tcgaccaaac aaggatttta tagttgtcag
     4321 tagattcgca tctcgcttct cgcatctcgc atctcgcttc tcgcatctcg cttctcacat
     4381 ctcgcttctc gcttctcgca tctcgcatct cgcttctcgc ttctcgctct accttagctc
     4441 cgacctcctg aatctgtcgc ccctacctct aacctctcac ctctcacctc taacctcaaa
     4501 cctcaaacct ccccaatgat ctcagtcatc ccaccccctg attttaataa aatccctttt
     4561 tttcacaata gcttatctct gcactataat ttgaacgtat gaaaaaatca acttccaaaa
     4621 aacccgctgg caagacacct aaatacgtct acttttttgg cgggggccgg gccgacggca
     4681 atgaatccat gaaaaatctt ttgggcggca agggcgccaa cctggccgaa atggccggcc
     4741 acccggccct gcggctcccc gtacccccgg gttttacggt gaccaccgag gtctgcactt
     4801 attattatga ccacaagaaa tcctatcctc ccactttgga tgccgaagta aaaaacagca
     4861 tcatcaagat ggaaaaactg acgggaaagg tctttggtga tcttaaaaat ccgctcctct
     4921 tgtccatccg ttccggcgcc cggcgttcca tgcccggcat gatggaaacc gtgctcaacg
     4981 taggcctcac tgaaaaaacc atccagggcc tcatcgacca gtccgggggc aatgaacgtt
     5041 ttgcctacga cgcctaccgc aggctgatca tgatgtatgc cgacgtagtg atggaaaaag
     5101 gggccggtgt ggaagtgaaa aacggtaaag gcatccgcaa gaaactggat gaaaaactgg
     5161 aaaacctgcg caactcaaaa aattatccca gtgacgattt tattccggcg gccgatctga
     5221 aagaactggt caaagacttt aaaaccacca tcaaagccag tctcggcaaa gccttccccg
     5281 aagatccctg ggaacaactt tggggcagca tcggggctgt cttcagcagc tggaacggca
     5341 aacgggcggt cgaataccgc cgcattgaaa aaatcccgga tgcctggggc acagccgtca
     5401 acgtgcaatc catggtcttc ggcaatatgg gcgacgacag cgccaccggc gtggccttca
     5461 cccgcaaccc gggcaacggt gaaaaccgct tctacggcga atacctcgtc aatgctcagg
     5521 gcgaagatgt ggtagccggc atccgcaccc ccgcccctat caatgaatat tccaaaaacg
     5581 accagagcaa acacctgcct tccctggaaa aacggatgcc caagctttat aaagaattgt
     5641 ttggctacca gaaaaggctg gaacaacatt accgggacat gcaggatata gagtttacca
     5701 ttgaaaaagg aagactctat atgctgcagt gccgggtggg taagcgcaac ggcatcgccg
     5761 ccgtacgcat ggccaccgaa atgcatggcc aaaaactgat cgacctggaa accgccatcc
     5821 ggcgggtatc cccgaatcag ctggtggaac tcctgctgcc tatgtttgat cctaaagtgg
     5881 aattggtcac caaacccata gccaaaggcc tgccggccgg cccgggagga gccgtaggca
     5941 aggtcgtatt ttcatccgat gaggccgtcg cctggagcca gaaaggagaa aaagtaatcc
     6001 tggtcaggga agacacctcg cccgaagacg tggacggcat gcacaaatcc caggccatcc
     6061 tgaccacgaa aggaggcatg acctcgcacg ccgccctcgt agcccgcggc tggggccggt
     6121 gttgcatcgt cggttgcgga gagatcgaaa tccaccatga atccaagacc ttcaagacca
     6181 aaagcggcat caccgtccgc gaaggcgatt ggattagcct caacgggacc aaaggcctgg
     6241 tctatcacgg ccagctcccc ctcatcgcca ccgacctggg aaaaaacaaa tcctataaag
     6301 acctgatgcg cctggtggac aaagtgaaag taatgggggt acgcaccaat gccgacaccc
     6361 ccaaggatgc cgcccagggc ctggcatttg gcgcggaggg tattggcctg ttccgaaccg
     6421 aacatatgtt ttacggtgaa ggcagcgacc ggcccctctt tctcctgcgg aaaatgatca
     6481 tgagtaaaaa cgagcccgag cgccggaccg ccatcaatga attgtttgaa ttcgtgaaga
     6541 aggataccaa agctaccatg gcggtcatgg ccggacaccc ggtcaccatc cgcctcctcg
     6601 acccgcctct tcacgaattt gtgccccacg acagcgaaaa actgcaccag ctgagtaaag
     6661 agctcggcat cagcatgggc atcctccgca gacgggtgct ggccctgcat gaaaacaacc
     6721 cgatgctggg tcatcgcggg gtccgcctcg gcatctccta tcctgagatt accgagatgc
     6781 aggtccgcgc catcctcgaa ggcgcagccg aactgatcaa acaaggtaaa aaggtcttcc
     6841 ccgaaatcat ggtccccgtg acctgtagcg caaacgaact gatccaccag aaaaaaatcg
     6901 tggacgccat ctataaggag gtttgcgcca aaaagaagat caataaaatc ccttattcct
     6961 acggcaccat gatcgagatc cccagggctg ccctcaaggc cgggcacatg gccgaggtgg
     7021 ccgatttctt cagctttggc accaatgacc tcacccagat gagttttgga ttcagccggg
     7081 acgatgtggg gggcttcctg cccgtctacc tcgaccaggg catccttcat gacgatcctt
     7141 tccagaccat cgaccaggcc ggggtaggcg aactgatcaa attcgggacc gagcgcggca
     7201 gggccgtaaa acccggcctt aaagtgggca tctgcggaga gcatggcggc gatcccgaaa
     7261 gcgtcaaatt ctgccaccgg atcggcatga cctatgtgag ttgttctccc ttcagggtgc
     7321 ccatcgccag gctggccgcg gctcaggcgg cgctggagaa tccggtcaag cctgttagga
     7381 aaaaataagt ttaaaccatt tgagtcgaaa caagccatag atttcctggt aagggggatt
     7441 tatggcattt ttttaataaa acagcaatcc aagttgttcc cattagcacg attctcgttt
     7501 cttgcttctc gcttctcgct tctcgcgatt tattccaact acaaacgaat atatccgact
     7561 acattcgaca acaaacggtt aatgcccgac tacaagcgtt taataaggct taattttaaa
     7621 aaaatcagct aatgtcttga aaattatagg tttaggttat actttagcaa aacggcagat
     7681 tgaacacccg ctgattcagg cggcctaaag gacttatctg taaataaaat ataaccacta
     7741 tgaagatctt tatatttcct gttgaatctc aaggccaaaa acgtatcggt atccggaccg
     7801 aacaatttga tcctgcatga cacaataatg actttaagaa atgagattgt tcacaatgct
     7861 tcttatgact acatgccaaa ggtctatcaa atattaaagg gaggtatgtt agtagagaaa
     7921 tatatactac ttccagactt acaaaatggt aggctgaaag tatttaaaaa tcgcaaacga
     7981 ttttttgatg acgacataaa actgaatgaa gttttacctt cgctgacaat tgaattttgg
     8041 caacgattaa aattaacaat tgatagtatt aaatgaactg tcttttttgc aaacaaaata
     8101 gcgacaactc aaaaagtgtt gaacacatta ttcctgagag cttaggaaac aaagaccaag
     8161 tattgcctaa aggactggtg tgcgacagct gtaatcaata ttttgcaaat aaagtagaaa
     8221 agccattgct tgaacagcct tattttagaa atgttagaca cagagcaaga atagagagta
     8281 agaaaggacg agtgccaatt gaaaatgcat ttatcatttc acctgaactt gcaaaagcag
     8341 acatcattat tgaccgtaaa gaggataaga caatcatcgt gatggaagat gaggaaaaag
     8401 caagacgaat tttttccagt ccaaaaggtt ccatgattat ccctgcattt gataaaccag
     8461 aggataacaa caaaatactt tcaaggtttc ttgcaaaggt tgcggttgaa ggacttttac
     8521 attggcttct aaaagaagat ggatggattg atgaagtaat gacaaactca ggacttgacg
     8581 agataaaaaa atatgctcgt tatggacaag gagttgaatt ttggccatac caccagagac
     8641 gaatttattc agaagaaaca cgatttaatc atcctctaat ttcaattcag gattacgaag
     8701 ttttacatga gtttaaaata cttccgacca aagagagcca ctactacttt gtaattgcaa
     8761 tcatgggaat agaatatgca attagtttag gtggacctgg cacggacagt tacgaacaat
     8821 ggctaaaaga aaataactat gcttcaatac ttgacgaccc aaatgaaaag aagagcagcc
     8881 gctaacaagg gcttagcgat agcaggcggg cagtggttaa ttgaagtgct gtgcttcttt
     8941 ttacatttgt gcttgctgac aggttagtgc ttctaaatgc ccgccatcgc caagccccga
     9001 aacgttggta gcaatagggc ctggacaaat tgtgtttcaa aaaataaaag gacgacactc
     9061 ttctaacaaa gatttcccat ggggacaagt aaacggcttc gcagacaact tggactttaa
     9121 aaaattaaaa cgtgggacat actaaaaatt aatacagccg acccttcgca tttttaagaa
     9181 gattgtgctt cgacttgtcc cgtccatttg gcacatgcca ttgccgctca gacccaccac
     9241 gacaccaaag ctatggcaag cgccaaatcc ccaccgcacc ctgttccaaa tcatttaccg
     9301 atcaagggtg caacttgtac cctgcttata aatctaatcc gtgacacaaa taggcttgaa
     9361 taggctttaa aaagtgttag ttttgtattg tgttttacag acgaaaaata atactagccc
     9421 ttttgcaact ctttgacggt cagatggata aaatcaggtt acaaaagttg ctttttttgt
     9481 tttcccttaa acaaactaaa gccgaatatg actttgtgcc gtacaaatac ggttgttatt
     9541 cctactcggc taatgccgat ttaaccacaa tggtttccaa aggtcttttg gcagaatcgc
     9601 aaaagcattt tgataagcta gacagaacag actatttgaa attggtaaaa cccgatgacc
     9661 tgaaacgatt acaacaggta aaggcagagt atggcaaaat gagttcaaaa gtgctgatga
     9721 agcatacata cattaactat ccgttttacg ctacaaagag tgaagttgcc ggagatattt
     9781 taaccgagac agaactacaa aaagttaatg cggcaaagcc caggaacaac gaaacagttt
     9841 tatttaccat tggctatgag ggaatttcgt tggaagaata tttagtgcgc ctgctaaaaa
     9901 aagatgttaa ggtcttagtg gacgtgcgta acaatccgtt aagcatgaag tacggattta
     9961 gtaaaagcca actaaaaaga tactgcgaaa acttagggat aaagtatgta cacattccgg
    10021 aagttggaat acaatcggaa caacgacaag aactaaaaac ccagacagat tatgataaac
    10081 ttttttccgt gtacaggaag aacaacctga ctaaaacggt tgactcgcaa acacaaatcc
    10141 tgaatttatt aaaagaatat aaacgcattg cgcttacctg ttttgaagcc aacatttgcc
    10201 aatgtcatcg taagcacttg gccgaagcca ttgaaaccct tcctggcttc cagtataaag
    10261 ttgaacacat ttaatctatg gctagaaaga aagtcctcat atcggtaaca acttatccgt
    10321 taccatccag aagttatgat gaattggttt gtaccgcagg acttttagaa actggcgaat
    10381 ggatacgtat ctatccgata ccattaagtt ttttgatggg tcaaagacat tctggcaaaa
    10441 tagaatcatt caagtacaca tggattgaac tcgatttgaa caaaaggaca gatgacttta
    10501 gacctgaaag ccactcgcca cagaattatg attttcgcga tatcgttatc tatgaccgtg
    10561 taggaacaga aagcaattgg ttgaagagga aaaaattttg ccttcaaaat gtttacacga
    10621 atcttagcca gttaattaaa gactcagagg caccaacaaa tatgtcgctt gccactttca
    10681 aaccaactga aatagtagga tttgaaattg aaaatgatac aagggaatgg aaggacgaat
    10741 gggttgaaat aagaaaacaa ggtgaccttt ttgctccaga aaaaccaccg gagactctta
    10801 ttcctaagtt gccatacaaa ttcttttaca gatttaaaga tgttgccgga aaagagagcc
    10861 gcttgatgat tgaagattgg gagataggtg cattatactg gaattgttta aaagcaaccg
    10921 aaggaaatga agccgtagcg ctggaaaaag taagagaaaa atacgaaggt gagtttcttg
    10981 cagaaaaaga tatttacctt ttcctaggta caaccaaaga atggcacctg cgcaggtcaa
    11041 aaaatccgtt tgtgattatt ggtgtattct atcccaagaa agaatctcaa atgaagttgt
    11101 tttaatgaac cttccgaaat catcggacag atgagaccca cataaatcgc acacacattg
    11161 cctagccgca catggagccc acaaccaaag ccagtcaatg agtgtgctca gtttagtgta
    11221 agtggacaaa gcgattaatg aaaaatgccc ctccacacga cagacaacca agattggtgt
    11281 aggataaatg gataacaaaa gtgagggcta cgagaatgaa gttcggagaa gggccctaca
    11341 gctaccaaca aaatgtttct gccatggtgc cgtaacgagt tggttttaag ttttatcttc
    11401 gttcaacgtt ttctttttca tgggactgga tgcagtttcg gaaaggcacc acagcagaaa
    11461 cacaatgtta tggccaatta ataaaaatac gagcatgtca gatagttacc acgtaacgaa
    11521 tagagatttt aaaggataca ccaagaaaga attagatgag atggcatctg accctaattc
    11581 tcttttgact gaatggtcga agaaaagagc cttgaaaaaa acaataaaaa aagccagaaa
    11641 ggaaatgagc aattctatta aattgacttc cttaatggat aaacaaatgg ttgatgagat
    11701 tgaacaattg aaaaagaata tgttttcgga ggattgggag ctcgtaaagt cctccgcaga
    11761 taaacttgga gagattggcg gaaacgagat tacagacttt ttgatttctc tattattttt
    11821 agacgattca ggtattagag atagagcagc attagcgctt gaacagattg gtgataataa
    11881 agctgtggag ccattattta aatcaatttt caaaaatcac aattacaatg ggactatggt
    11941 ttttgccttg gagtcgttgg attgcagcaa acatcttaaa gacatattta aaattctatt
    12001 tttcgaggct tatgaatcca aaatgagtgc aatggcgatt cttgacacgc agatttttga
    12061 attcacaagt caggatttaa ttgaaattgc taatatgtgg gaggattgca aattacatcc
    12121 tgagaaatgt cctgagattg aagatgacga tgtgagaaag gaaatgcagg aaagtgtaaa
    12181 tggatttttg gaatatttaa aatagaatta actggccata atcgaatagc cctgcacttc
    12241 ctgctatcgc tgacaggggg aggctctcac accagattcc atatcaaaga aaagcagtat
    12301 tttcgataac cccttacagc tattccaaat cttccagccc aatgaccgat catgaaattc
    12361 gcaacgctga cctttctcct ggcagtccct tttgtactta tggcccaatc ggataaaaaa
    12421 tgacggagga ttttctccag tctttcgcgg atgctttcaa tgcgcatgac ctggaagcca
    12481 tcctgtccca tatgaccgcg gattgtgtgt ttgaagcttc ggcgggaccc gatgccgacg
    12541 ggcagaaata tgtgggccag aagcaggtaa aaaaagcctt cggggatgtt tttgacaact
    12601 ttcccgatgc tcagtggaac catccccggc attttatctc cggagaccgg ggcttttcgg
    12661 aatggacttt taccgggacc aagaaagacg gcaccagggt cgaggtcacc ggatgcgacc
    12721 tgtttacttt cagggacggc aagatagcca tcaagaattc ctaccgaaaa aaccggctgg
    12781 aaaccaggta aaatcctttt catccggtcg gaatagaaga atttgacgcc aaaaaactaa
    12841 accatatata aaatgaaaat caacgtaatg ttcatgatcg cggcggtcta cggattcgtt
    12901 gtcgggcttc ctgtaatctt tgcaccggaa gcaacggctt cgatgggcgg tatccaaatt
    12961 ccagacggaa tgtcgatgag tctccgtttt ttaggcgttg ctgaattggg gctgggcacc
    13021 attgcctggc ttgtacgcaa cgccgaagcc tcaaagaccc gcgacggggt ggcgctcggt
    13081 ttcaccattt attttggcct tcattccctg gcaagctggt atggtcagtt taccgaccct
    13141 tctaccacta tgcattgggt catggccagc ttacaagctt tgatagccgt tggctttttc
    13201 ctggcaggca gggctaatag gtcgaaaagc ggaaattaaa actccattta atcccccctc
    13261 cccttatttt gagttccgct gcggattcaa gagtgacaga tctatcgaaa ccaggaggct
    13321 tttataccag gcagaaatgg tgaggcaggt gatcgctaag gctctataat tgatttgatt
    13381 cgtactagct gaattatttt gggtccctgt gaaatatccg gcccatccct tgaaaataac
    13441 cggccaaatt catatattta gattatccgc tatcgggtaa gtggaggagt tttttattat
    13501 tggtttgaaa gcgggaaaga cgaatcgaca accgaaagtc ggaaggactc gatgatctga
    13561 gtgatcctga tcataatcgg ttaattgcat aggttggcga ccgctgtcct gccggtttat
    13621 aaatgtcaga agaatatact cttgttactt aagccttcct ttatatgaaa aagccaaaaa
    13681 atcttgcttg tatggttggc atttgccatg ccctgctgct tatatcctgt accgccccag
    13741 ctggttcttc cggtctgagc gcgacagaca aagaatatat ccaaagtctg acccaaacgg
    13801 ttgaggagaa ttggaataag ggaaaccggg agccttatgt caatcgcttt acctccgatg
    13861 ccttttacat ggcccccaat atggaaagct tggttggaaa ggatgcgata aagagctttg
    13921 ccgcttcctt ccctgagtta aaattggagt tcaccattgg agaaatagag ggatcttcgg
    13981 aatttgccta tgtgcggggc ggatatctgc ttaagactcc acaggactcc ctttttgaca
    14041 aggggaaatt tctgagcatt tgggaaaaaa cacccgaaaa aacctggctg ctgtcacacg
    14101 acatgttcag ttcagacctg ccgcccacaa agtgactttt taaaatcata tgtccacggg
    14161 ggtccaggga ttcaaaacac ctctgacctt cccggaatac cgttcagcca aggaacttaa
    14221 tcatagattc atttcgtatt tttataactt catcccgccc tgttgtttaa ttccaaggtt
    14281 aaaattccct ttgaataaaa ttaggttccc tggaccaact caaataatat aaaggactat
    14341 tatccgggtg tttcaatccc agaaatcaat gaaagtcaca gatcatggaa aatttaaacg
    14401 aactcatgct gaaggcagcc tttaaaagcg aatgggagga ggtggaaaaa tgggtttcac
    14461 ggggagcgga tataaactac cgggaccctc atggaaattt tgctttattt acggctgcct
    14521 gggaaggtca tctgccagcc atggatttgt gttacggttt aggagcgcaa atatcctttg
    14581 aagacgccaa cctgctttgt aatgccgcct acaacggcaa aacggattcc gtaaactggc
    14641 tgttgaccaa aggagaaaac gccgatttca gtttcaggga tacgggggaa aacgccctgc
    14701 attataccct cagtaaaacg tctgagatgg atgaaaggac ggagatcgta aagatcctga
    14761 tcgaggccgg gacccaggtg aataaaaaaa ccatggcggg acagccgacc ctctgtttca
    14821 tgcgggacgc ctggctcaaa ggggaaaccc cactgcaccg tgcagcggcc tatgccgatg
    14881 ccgccattat aaaaatgctg ctggacgctg gcgcagatcc ctccgtgaag gatgcaaacg
    14941 gggatacgcc catgtcctgg ggaagctggc acttgagaga ggcatccgtg cttcgtttgc
    15001 tgcagtatga gggagtgccg cggattcatt aattttatac cagacagggg cttaccttcg
    15061 gttgggaaaa ggcaaggatg ccttgataat tccctgctga aaatcagact ttgagttttt
    15121 ccgaatcggg ctgaagcata aacatgccga tgaaatttat acccatcttg ttttttttgt
    15181 ccacctgttt agtcgggtct tgggctcaga atgaagcgcc ccgggaaaag gtggtgatca
    15241 cctccaaaaa gcagaaaacc cgggtagcga taaatgcaaa gggggcattg gaggtcaacg
    15301 tatccccgcg gaatatcctg aaatttaaaa ccaccggatg ggtccgctac agcgacttcg
    15361 gggccagggg ggacggcaaa gccgatgata tggacgccat cgccgcgacc catgcctttg
    15421 ccaatcagca aggcctgatg gtgaaagccg acgagggagc tacctattac attggcggca
    15481 aggaacgcac ggcaatcatc cggaccgata ccgattgggg aactgcctct ttcatcattg
    15541 acgacaccga agtgcaaaat cgcaacgctc ctgtctttat ggtcagctcc agccaacaat
    15601 cttttaaact tgaagggata tcctcactga aaaggaacca aaataatatc gacgccgccc
    15661 tgcccgggtc ctgcctcatc tcggtcacca atgcccacgt gaaacactat atccggtttg
    15721 gtctgaacca ggacaatgga tctccgcaga ccgatatctt cgtcgtggac aaaaacggca
    15781 aggtggatat ggatgggccg gtcatctggg actttgacca gatcacggac atcaccgccc
    15841 tgcccatgga cgagaaaacg ctgaccatcc acggaggacg gtttaccact atagccaatc
    15901 aagccgaatc gaaatacacc tattacagcc ggaaccttgc aatcaggcga tccaatgtag
    15961 tggtcgatgg actggaacac cgcatcacag gcgaaggcga ccagggagcg ccttatgggg
    16021 gtttcataca catctccgac tgcgcctacg ttacggtgca gaataccatt ttgaccgggc
    16081 ataaaaccta ccgtaccatc ggcgccgcag gggagccggt ttccatgggc agttacgata
    16141 ttttggtcaa ccgcgccctt catgtctcct tcaaaaactg cagccagacc aatgacatcg
    16201 acgaccgaac ctattgggga ataatgggtt ccaactactg caagaatctc ctgtatgacc
    16261 gctgcacctt atcccgcttc gatgcccaca agggggttgc caatgccacc atccgcaact
    16321 ccaccctggg acacatggga atcaatgcca ttggcagcgg gatcctaact gtggagaact
    16381 gcaccatccg gggaaggagt atcgtgaacc tccgctccga ctacggcagt acctggcagg
    16441 gagaaatctt catccgaaac tgcctttttg tacctgaagg aggcaggcca agccgggccg
    16501 tcctgatcag cgggtcctat tccggtcagc atgatttcgg ctacacttgt tatatgccgg
    16561 agcgaatcac cattgaaaat ctccatatcg acgactccag tcatccggag gattaccggg
    16621 gacctgccct tttcgcaaat tttaatccgc aaatgaccga tgattcctat cgggagtcct
    16681 ttccctatgt caaaaccagg gaagtcatac taaggaaggt gaccaccgcc agcggaaaga
    16741 ccctgaggct tagtgaccat cccttcatgt ttaaagaggt acaggtaagc tctgaataaa
    16801 aagcataaac tgaccgtctt ccaatccggg actttaaagc atcggggatt acctgaatga
    16861 gcctggggga caagcccatt tatatttcgt ttattgaatt ttgacggagt atgggctcag
    16921 gattcaatat ccgtactgaa aactgaagcg ataggccatt cacttaaata tgcccctgaa
    16981 cggatacagg cgaccacaag gcatggatcg ccaatgaata tgtcatacat tgccaaaaat
    17041 gccgacaaaa acggggtctc aacttttgga tgcctattta ttaaatcctc cgtgcacccc
    17101 gtgtactccg tgcgacctcc gtactccgtg cgccctcagt actccgcgtg ctcaaacttc
    17161 ccggatctgc accacctcga tctcatccat gctcaaatgc ttgccccagc cgcattcgac
    17221 ctcctttttc cgtccggtca ggcggaaagt gatctccacc cctatgctat aggtccccgt
    17281 aagggtcatg gctttttcga tcaagggata gaccaggtca ttgctctggt cgctggccag
    17341 atactggccc cattcgtccc ccatggccac cgcaaaatgc cagctgcagc cgtctacagc
    17401 aagctggttg aggagcaacc ctttcttggt aatcaaatcg ttttccgccg actccaccac
    17461 cgattgcgaa caagccaccg gaaacatcag gccaatcaaa atcactacgt actttatcat
    17521 tttctccaat atgttatagt atgcccttaa agacaggggg ctttgtggaa aggttggctc
    17581 aaaagcatga aatggatgga ttaatcgacg aaatgcatta aaatacagat ccgggatgag
    17641 atccctattt ttacatccaa atccatttaa taccgcctta acatgaagca ttttttctta
    17701 accttttctt gccttttaat gatctccctg cacgcccagg acaacaacag tttccccgtg
    17761 cgtacaaaaa tcgccaacgg gatcatcgaa gggaattacg acaccaaaac cggcctccag
    17821 caatatttcg ggatcccttt tgccaaacct ccggtgggca ccctccggtg gaaagccccg
    17881 cagcccctgg tcaactggac cggggtaaaa caaaccaaaa aattcggacc caggccggta
    17941 cagggcattg tcttcgggga tatgatgtcc cgctctgacg gcctgagcga agactgcctc
    18001 tacctgaatg tgtggacccc ggccaaacgg aacaccaaag gcctccccgt actggtctat
    18061 ttctatggag ggggttttgt ggccggtgac gcttcggaac cgcggtacga tggagccagc
    18121 atggcccaga aaggcatggt ggtcgtgacc accaattacc ggctcaatat cttcggtttc
    18181 ctggcccacc ccgaactgag cgccgaatca ccctataagg cttccggcaa ttacggcctg
    18241 ctggaccagc acgccgccct ggcctgggtc caaaaaaaca tagccgcctt cgggggagat
    18301 cccaaaaaag taaccattgc aggggaatcc gccggatcga tctcggtcag cgcccagatg
    18361 gtttcgcccc tgtcccgaaa cctgatagcc ggcgcgatcg gcgagagcgg cgccggtatt
    18421 ttcccaaccc tcgccccggt cccactcgcc caggctgaga aagaaggagc tgactttgcc
    18481 aagaatgcgg gttatccaag cctggcctcc ctccgcgcac tgagtacgcg cgaggtttac
    18541 gagatttacc aggaagcccg gcgtttcggt ttccccaccg taatcgacgg ttatttttat
    18601 cccaagacca tccccgaaat attcagggcc ggcgaacagg ccaagatacc actcctgctg
    18661 ggctggaact ccgccgagat cccgggcggg gcttttatgc agggttctcc ctacaccagc
    18721 gaaaatttta tcaccaaggt caaagcggcc tttcccgatg atgcggatga aattttgaaa
    18781 ctttacccgc acagcactgc gaaagaggtg gaatactccg ccaccgccct ggcctcggac
    18841 cgctttattg cctacagcac ctggaaatgg tttgacctcc accggaaaca cagcagccag
    18901 ccggtttacc gctatctcta cagcaaactg cgcccacccc tcgttgacaa atcccttagc
    18961 ccgggccttg ccggagggac tacccgcagc gaccagcctg cccctccccc gcctccggcc
    19021 attggcgccc ctcatgcctg tgaaatcgag tactgtatgg gcaacctcca cctggtcaaa
    19081 gaatacgcct ggaccagcga tgattataaa gtttcggaaa ccatgcagaa ttactttgcc
    19141 aactttatcc tcaccggcaa tcccaatggt gcaaagctgc ccgaatggcc cgccgcttct
    19201 cccaatgatc ccatgccgcc ggtcatgatc ctcgacaccg aatccaggtc cgttcccgcc
    19261 aaggatgatg gccggtatct gttgttggat aagttttaca agaatcaata agggggtttg
    19321 gtgggcaatg aatggtggga ctgattgcca tccaccatcc accaaccacc atccacccat
    19381 acctttgccc gatgttccct tcattcttaa accggcatcc ctccctggcg gtcaagaatg
    19441 cgttcattta cctcatcctc ctgatatttt cttcttcgct cattggatat tcgatttacc
    19501 gcttgagcgc ggcgctgatc ctgtcttcct ccaagaccca gatccgcaat gaagtgcggg
    19561 acatggctgc aagaatgacg gggcactttg actacatcgg gagggatgtg cggtttctgc
    19621 aaaataatcc acttatccag gaatacctga aggctccgga aagtcaaagg aaaaatctgg
    19681 cggataaact gggagatgaa ttcaaagccc tcctggaatc caggcctgcc tatttccagg
    19741 tccggtttat cggagtggcc aacaatggga aagaactggt acgggtggac cgggacgggc
    19801 gccaaggaaa aatccttgcc gaagcagagc tacaggaaaa gggggaccgt agctatttta
    19861 cggaaaccat ccgacaggcg gaaaacagca tttatttttc cgaaatcgac ctcaaccggg
    19921 agcacggtaa aatcgatttc ccctacatcc ccaccctcag gatagccggg ccggtgtata
    19981 aggagggccg tgcctttggc atcgtcatca tcaataccga tctcgacgcc ctgttcggag
    20041 acctcaaaaa agcgaccgga ctctacgaag acctctatat tttcaacaat tcgggttatt
    20101 tcctggtcca tccggatgaa gcggaatgtt tcggatttga attcggcaag ccgcccaact
    20161 taaaacattg gtttgctgac ctcaacgcgt tggatccggt atttaccagg ctgcctgaga
    20221 cggatagtat ggtgtcccgg gaaaaagaag tcaccgagtt ttacaccctg ctccacccca
    20281 agacggacta taaattgttt attgccctca gcgcgccgga aagcgccatc ctttacccct
    20341 tcaaatcctg gaaaaaaaac atgatggcgc tcacctttgc catcactttt ttattcctgg
    20401 tgatcgcctt tctctggatg aggaggcagt cccgcgaatt aaaaaaaatc acccggtcca
    20461 tggtgaagtt ttcagaagat tattcagaaa gcaaactgct catccggcag accgatgaga
    20521 tcggggaact ggccaaggcc ttttcgaaca tggccggaac catacgcaac aacgtgaagg
    20581 aactggagat gtcgcgggaa gctgcggtga aagccaataa atcaaaggaa gaattcctgg
    20641 aaaacatgag ccatgaaatc cggaatcccc tccagagtat cctgggtatg accaatatcc
    20701 tcgaaaccaa ccaccccagg cccgaccagg agaccgtgat caaaaccatc cgcttcagct
    20761 cccggcagct cttgtcgctg gtcaacgata tccttgattt ttccaagctg agaatcggcc
    20821 aaatacggct ccaggcccag gatacagacc tggctgacct gttgtcggag atcacgcgaa
    20881 gccatttatt cctggcccag tccaggaata tttcccttga gtcccatgtt cctcccggcc
    20941 tcaaagggac cttgttccgg gtggaccccc tgcggctcag ccagatcctg aacaaccttt
    21001 tggtcaatgc aataaaattt accaatcccg gcggaaaggt cgtgctggat gtagaggcta
    21061 atgccgggga ccaggccatg tccctgcggt ttaccgttac cgataatggc accggcattc
    21121 ctccggatga actggacaag attaaaaaac gcttttattc aagggatgcc accgaactga
    21181 atactcacct ttccggtgcg ggattgggtc tgcccatcgt gattcagttg ctggaattgt
    21241 ttcattctga actcgaagtg gaatcggaga tccaaaaagg ctccaggttt tcattcagcc
    21301 ttgaacttcc ggtgacccct ccggcagcca catcgcttgc aggccattcc tctaacaggg
    21361 gattgccccc cgggatcacc caggtattgt gcatggacga tgacccacag attatttatt
    21421 ttttccgtca ggtcttcagg actttggaga aggacctggt ttgcattgga gagccggggg
    21481 aactggatac cttgcccacg ggccggaggt ttgacctgat cattgccgat tttatgatgt
    21541 cctccggcaa tatgatccgg tccctgcccc gcctggcccg ccttaaatcg gaacacggcg
    21601 tgctgatcat actcagcggg atcgatgttc aaagccggct tggggaatcc ggcatgcagg
    21661 gagtggacct ggttcttcaa aaacccatct cttcatcagc cttgctggcc caaatccaaa
    21721 aagcctggga taatgcgcac agtcccccgc cctggatgga agattttttt aacgactatg
    21781 atcacgagga agccctgatc tcccatgccc tgaaaatcct cattgaggaa tggaagacca
    21841 tgcagggcca attgatccag tgcctgaacg cccgggacaa ggaggccttt gaccaggttt
    21901 tccataaaat catcaccacc ttgcggagat ttcgactgca ccatttgctc aaagtcctgg
    21961 aacagatcca ggaacagttt tcagtttcgc cggcggaggc cagggaaaac acgatcattt
    22021 tatcatccct aatggacaag acacggcaag agtttgaata ccagctgcat cacctgcctt
    22081 ccatcctcac aacgcattga ggctggcctc caggtcactc cggtattttt ccccaatggg
    22141 cactttttta tcccccagca cgatctcttc aggggtgagg taatcgatcc ggtccatatt
    22201 gacgatatag gagcggtgta cccttttaaa attgcggggc agtttggctt cgaggtcttt
    22261 caaagtaaaa agggaaaggg tctgggattc cgggaaaaca aaagcgacat aattatcctc
    22321 cgatttgata aaaagcactt cactcagttt gatgcggaca tttttattgc cgtctttggc
    22381 aaaaaaaaca tcggtgacgg ggccaggact ttccggaatg gctgactgac gcccggtgac
    22441 ccgctgcatc gcctttagga aacggtcgta ggaaaggggt ttcaccaggt aatccaccac
    22501 atcctcataa ttataactac gggcagcaaa ggcttcatca gaggtcacca tgatgaccgg
    22561 caaacgactt tttttcagct ccaggaaatt ctggccattc atatccggca ggttgtaatc
    22621 caggaagacc aggtcgatct cccccccggc catcaggcgc aaaccatccg tcgcggtcgc
    22681 ggaaagaata caataatcca ccagaccgga tttggtgcaa aaatgtttca ggaggtcccc
    22741 gatcaggggg tcatcgtcga tgatcagaca tttaagaccc atccttatat ggtttaatat
    22801 caatgataac ggccggattc aaccgggaca tggccctttt accctttttt ttgccggttt
    22861 taccctttcc ggaggggagt tcgtccaatc gggcctgatc gaaagacatc cctggatagc
    22921 tttgaggctc caacgacagc tgaaatgata ttggccgctc tctcaccggt ttcgttaatg
    22981 ccttcacaaa gatggggcat ttcccttaaa agtgaagccg gcggatccat aagcaccttt
    23041 gatcttcctt tatattttca atacaataag ttttttaaga gtcttttaaa ggggaaaaaa
    23101 cggcgagccc accactcctg tttttcccct ttttcccttt ccggactccc gcttgctgta
    23161 tgttttcaat gaatctaaaa tattttatga ccaggatatt tgctgcagtt cttttattcc
    23221 gggtacttct attccctgct ttcctttcgg cccagcagcc gaacgatgcc atctatatgg
    23281 ataaaaatgc gatctgcata gtggccgctt acggccacaa cagttgggat acctattggg
    23341 aaaatactct gaaaaggcag aatctgaata tcggcaccaa taccacccaa agctttgcgt
    23401 tgatggccgc tgccggcatc acggacaaac tcaatctcct ggtaggcctc ccctatgtga
    23461 gtacccgcaa cagcgccggc aacctccttg gacaaaaagg cctccaggat ttttctgcgg
    23521 ctttgaaata caaattattc aataacgagg gcctgtccct ccatggcact ttgggaggat
    23581 ccataccggt gagcaactat gttcctgact tccttcccat gtccattggc ttgcattccg
    23641 ccacggcctc ggcccgattg gtcttcaatt acctgcataa atcgggaatt tattttacgg
    23701 ctcatggagc ttacaccgca aggtccaacg tcaggacgga ccgggattct taccaggcca
    23761 acgacaaaat atataatagc gatgagctgg ccatacccaa tgccacggat ggaggcatca
    23821 atatcggata cctgaaaaac tcccttcagg ccactttgtt cctggaaaga tttgattgcg
    23881 ttggggggga tcatatccga aggaacgacg ccccttatcc gacgaatgat atggaagcca
    23941 ccaacatcgg cgcatatgtc aaattccagc caggccattt cggcgtcaat gcccgatatg
    24001 cttatactac cagcggtaga aatgttggac aaacgaccag cctgatggcg ggtatccttt
    24061 accagttctc cattaaaaaa tcctctaatt aacttatcca ataaatccat ttcaaatccg
    24121 gggatatgaa aatgaaactt tttccagtga tcgccttggt gggagcattc atagtcgcct
    24181 gtgacaagga tatccaaaat ccgcttctgg aaccttatgc ggtctccagc ctcgacgaaa
    24241 acgcaggcca atggaagcct tatattttat ccagcgtcag cgatatcgtg gtacccgaac
    24301 ccaaaccggt gaccgatccg gcatatctca aggaattgga ttcactgaaa aattatattg
    24361 ccccaagagt gactccagaa aacagggctg ctgtcgaaca ctggggagcc ggggccgttt
    24421 accggtggaa tgaaatagcc cgggaactca gcgccaggta caatcttccg cccgcctcca
    24481 acgccgaagg gaaatacccc gttccggatg cagcgaatcc gctcaaagat ccaaaattcc
    24541 catttgcaaa tcccccttat gccgcccgcg ccctggccta tctgtccgta gcccagttcg
    24601 atgccctggt cacggcctgg aattataaat acaagttcaa ccgcaaggct ccctacaaaa
    24661 tcgattcagg catcaaaccc cttcttccgg cctcagacct tccttcttat ccatctgaag
    24721 cagctgtggt ggcagccgct tcctataccg tactgatcgc tatgttcccc ggcgaagttc
    24781 cctatctgga cggcaaactg gcagaaaata aaaatgcaat gatatgggct gctatgagta
    24841 cacccagtga tattacagca ggcgatgcct taggcaaagc tgttgcagcc agggtcatgg
    24901 cgaaagccaa aaccgatggt atgtccgcgg caaacaacca gtccaggact cccgcaatga
    24961 ttgagaccgc caagtcaatc gggattattg aaccctggaa aagccaggag agccctcttc
    25021 ggccgacgat gctgcctacc tatggggagg tggcaacctg gaatttcagc aaggagacca
    25081 tgattgctat gagaccaccc aaaccctgga tcgtgggcac gcctgaattt gaaaaagacc
    25141 tgaaagaact tcagggtatt caaaaaaatc aaacccgtga acaagcccgg atcgccaatt
    25201 tctgggcaga tggagccggc tcctacaccc cccctgccca ttggcaccgg attggggcgg
    25261 aggccggtaa agatgccaaa tacagtgaag tacgcatggc ccggaccctg gcattgctgg
    25321 gtactgcaga aatggacgca ggcattgcct gctgggatgc gaaacactat tattttactc
    25381 ccaggcctca gcagtttggt gttaaaacat cagtaggtct gcccaatttc ccatcctata
    25441 cctcgggcca ttccactttt tcttccgccg gtgcgaccgt gttgtcctat atttttccgg
    25501 aaagaaaaaa tgagtttgaa gccatggccc tggaagcctc gaattcgagg atctatgggc
    25561 tgatccacta ccgcgtggat tgcgaaatgg gtgtagccca tggcaaaacc atcggtggat
    25621 acgcggtcca acgcggtcag accgacggtt caggacttta atttattttt ccattaaacc
    25681 aacaggaaat gaaaaaatat tcaaccatcc ttaccttatc tctcctgtcc gtcctcgttg
    25741 ggcatgatgc tcttcaggcc cagggatgca tcgcggtgcg gcatatgtct tgcgccacgg
    25801 gcgggatcaa caataccaat accgcaatga ataaggggca gttccagttt gttgccggtt
    25861 accggtacct gcattcgtat aaacattttg tgggtacggt ggagcaaacc caccgggtcg
    25921 aacaaaatac ccaggtcatc aataatgcgc attcctttga tttcggcctg acctttgcct
    25981 tgaatgaccg cttcagtttt gcactcaatg tcccttataa cgacaacgcc aggtcctcac
    26041 tgtatgagca ctatggaaat gcagaatcgg ccaacccgaa ccgcagccgg ttttcaacct
    26101 attcttcagg catcggggat atccggctga ccgctaccta ttggatattt catccccaaa
    26161 aaagcatgaa tggtaatgtc gcggttggac tgggcgtcaa agcgccgacc gggaacccga
    26221 atgcaaaaga taattttcat aaactggata aaaacggaaa agaatacctg cagtataaaa
    26281 cggtggatca gtccatgcag gttggtgacg gaagctgggg atttaatgtg gaaatgcagg
    26341 gttaccaggc tttgtttaac aatacttccc tgtattacaa tggtttttat ttattcagtc
    26401 ccaggaatat taacccgctc accggtttct ccgtggccga ccaatatgcg gcccggctcg
    26461 gactgaatta ttcgatactt ccaaaacaag gtattgccgt cagcctgggc ggacgcatcg
    26521 aaggactgcc tgcgattgac ctgatcggcg atagcgaagg attccggaga ccgggttata
    26581 ttatttcggt agaacccgga attttctgga tgaccgggcg ccataccttt gccttcaacc
    26641 tgcctatcgc cgtagaaaga aaccggatca aatcctggca agaccagcag gaccccctgg
    26701 gcctgaggca cggcgacgcc gctttcgcgg ataattttat ttcagcaact tattcctacc
    26761 gcttctaaag agaagccgta atgaccttaa aacaatagtc tttcatacaa atgttccaca
    26821 gctggcagca gtttattttc aaatgagctg ctgctcagat tgtgttggat tccttccaca
    26881 ggattcaaag gtcattattt tgccgaaacc ggctcccgcc ggctcccgcc agcacacgtc
    26941 attcttaatt cttaattctt aattcttaat tttttaaaac aagccccagt gccccatatt
    27001 ccagtagaag aacatttgcc cggcatcacc ggattgctgg aatatcgcaa agacactgca
    27061 gctcccatcc gtgcattgac ccaattcctg ctgagaggca gttccagctt gtctgcagga
    27121 gagcgggaac tgatcgcctc cctggtctcc tcgcgcaatc aatgtgtttt ctgcacccag
    27181 gctcacacta ccgccgccga tatcctgctc ggtgaagcgg aaagctgcag gcaggtcatt
    27241 gaaaactacc cgcaagctcc ggtcagggaa aaattaaaag ccctgttgca tattgccgcc
    27301 ctggttcagg taagcggtaa aaaagtggat agcgccgcca tcgcccaggc caggcaggcc
    27361 ggcgccagcg accaggagat ccacgatacg gtccttttgg caggactttt ctgcctgtac
    27421 aataaatatg tcgatggcct ggccaccggc ttgccatccc atccggaata ttttaaacaa
    27481 ctgggagaac gcattaccac catcggatat gtgcgccttc ccggcggtta tgatcatctg
    27541 aagaaaaaat ccgggtccga atcctccaaa gatttataat taaaaatttc agataaacct
    27601 atttatgaaa aacctgttgc tttttaccct gcttttaatc accaatgtcc ttattggaca
    27661 gaatattacc ggtaaggtcg tggacgccca gactaaaaaa ggactcgaag gagtcgcggt
    27721 tgccatcaaa ggcagtgccg aaggtactta taccgatgcg caagggatgt tttcaatccg
    27781 tacttcggag tctttgccgc ttaccctcgt cacttccctg gttggttatg aatccatgga
    27841 aatcaacgtg agccagcctg gacccgtgga aatcgtcatg agatcaacca ttgaagaact
    27901 caaccagatc gtggtctctg catccaggat tgaagaacgg ttgctccggt cccccgtggc
    27961 cattgaaaaa atggaccttc gttccgttca acaaacccca tcggccaact tttacgatgc
    28021 cctggtcaat ctcaaatcac tggacctggt gacctcaagc ctgacattca agcagatcaa
    28081 tacccggggc tttaatcata cgggcaacgg gcgctttctc caattggtgg acgggatgga
    28141 caatcagtcc gccggccttg gattcgccat gggtaacctc ttcggcccgc atgacctgga
    28201 tgtgtccagc gtggaactga ttcccggggc cgcctcagca ctctatggtc cggtagcttt
    28261 caacggaatg ttgcatatcc aaaccaagag ccccttcgac ttccagggac tcagcgtcca
    28321 aaccaaagcg ggcttaaacc atatcagcga cggcaccggt gaaggggcga aaccctttta
    28381 tgacctgggt atccgctatg ccaaagcctg gaataacaaa ttcgccctca aactgaatgc
    28441 ctcctacctc gccggcaccg actggtatgc aaacgactat acggacattg accccaacac
    28501 tcccgaaggc agcaggggcc cgaataatcc cggcaggaat gcgctgaata tttatgggga
    28561 cgaagtagcc caaaccattg aaggcatagg ccgggtatcc cgtaccggat atgaggaaaa
    28621 ggacctcgca aactacgacg tctccagcct gaagttcaac ggtgccctgc actatcgcat
    28681 ttctgatcaa ctggaagccc tctaccaggg gaatttcaac cggggcgtag cccaatacac
    28741 cggcagcagc cgttttgtga tcaatggctt caacctggtc cagcaccgcc tggagttgaa
    28801 aggcagtcac ttttatctca aagcgtactc cacccgggaa aattcccagg attcatacaa
    28861 tacccgttct ttgggacagc tcatcaaccg aagctgggta aaagacctgt cgggagccac
    28921 cgtcagcccg gcgaatgcgg atgccacctg gttccagcgt tatgcggcgg cctataaagg
    28981 ccaggtcaac ggaacggaat ccaacaacca tcttgcagcc cgggcttttg ccgacaacgg
    29041 ccgctttttg ccgggtacct ccgctttcga gagtgcccgg gaccggctga tcaataccca
    29101 gggactgcaa ggcgccggca tcctcagcaa atgcgggatc aaacatgctg agggcatgta
    29161 tgatttcagc catttgttgg ccaatgcatt tgaactccag gttggcgcca attacaggat
    29221 ttatgacctg aataccgaag gcaccctctt tgacgataag aataaaaacc tgaccaatga
    29281 cgaaatcggg gctttcgtcc agctttccaa aaacctgctg aaagaccggc tccggctgat
    29341 tgtttcagga cgttacgaca aaaatgaaaa ttttgatggc cgactgacgc ccagggcctc
    29401 ggcggtatac tcctttaacg ggaacaacca cttccgcatc tcctatcaaa ccggattccg
    29461 gaatcctacc attggagacc agtatatcaa actgaatgta gggcccatca tcatcctggg
    29521 cggtgctccc gtaaacgcgg cgggcctcaa tgcttacgaa aactctttta ctgccgcctc
    29581 ggtcggcgcc tttggcagtg gattcggtgc acaaatcgca caaggggtcc ccttccccca
    29641 ggcgctcgca aacaacaaag acaaattggt caaatccaat gtcccctata tccagcctga
    29701 acgcgtgagc agcattgacc tgggttataa atttctgctg aatcagcgcc tgctgatcga
    29761 cctgggttat tataacagcc gttaccggga cttcctgatc aatacggtcg tcatccggcc
    29821 tgaccatgat gtactggcca acggatcaat taatccggcg gccgctgcgg atatccttaa
    29881 tggtaaaatc caggcttttc agttgtatac caatgcagcg gatgaggtta cggtccaggg
    29941 ccttacttcc ggactcacct actttttacc caaaaattac cgcctgatgg gaaacgtgac
    30001 ctggtcggat ttcaatttgc tcaatgcgga cccgaataat atcccggcct tcaatacccc
    30061 ggaatggaag accaatttga cgttcagcaa tcccgccttg accagtaaat tgggcttcag
    30121 cgttgcctgg cactggcaga acgcattcga ctggtatggt accttcaccg gcctgatccc
    30181 gggaagggtc gatgcctaca gcctgctgga tgcgcaggtc agttacaaac tgccatcgct
    30241 caaaaccacg gtgaagctgg gtgccagcaa tataaccaat caatacatcg tacaggctta
    30301 cgggtccccg gcggtaggag ggatctatta cttaagcctt accttcgatg agttgatgcg
    30361 ttgatccggc catgaaaaaa atttctgccc aacaccaatg gacccttggc cttagcctga
    30421 tcgcatacct ctttgccggc acggtttcca ccctcgtttc cgtttacctt ccagtgatcg
    30481 taaacgacct gaaaggctac cgggtcccgg aacaggagtt gggaaccctg ggcgcctttg
    30541 taaatgcctt tttcctgtat ggctggatgg ccggaggcat tctgatgggt ttgcttggcg
    30601 accggtttgg aagaaaaaag atccttgcgc tctccaccgc tttatacgga gcagctaccc
    30661 tgctgatcgt ttttgtgcac gattggcgac tgatgatgtt ttacagaatt ctggccggtt
    30721 taggcgttgg gggtactctg gttttatcca cggtcatgat ctcggaaacc tggccggaaa
    30781 aaagccgggc ggtgatccag ggcattcttt ccgtttcttt tcccgtaggc attgtggcta
    30841 cgggagggct caatattctt tttgaaatat ggcgcagggc tttctggctt ggcctgatcc
    30901 cgatcctggt cgcgacattg atgatcctgt tccttaagga accggctacc tggaaccggt
    30961 cagcgtcaca gggtacaaaa ctcaaagcgc tgctccatcc accggtacgc tataacctga
    31021 tatcgggcat attgatcttt ggttcggtac tcattgggct ctggggactg ttttcctgga
    31081 tgcctacctg ggtgcaatcc atcttgcctg agggatatgc ggggacccgg gaaagagggc
    31141 tcaccatgat gttgttgggc atcgggggca ttaccggagg cctgttctcc ggtttcctcg
    31201 tacgggtcct gggtgcgcgc aaaaccctga gcctcgcttt caccgggctg accgcggtaa
    31261 gtttgatttt gtttcttacc aatccgcggt tttcagagat catctatccc gaaaccgttt
    31321 tgctggccct gttttttggc ataagccagg gctccctatc ccattacatt cccgaattgt
    31381 ttccaagtcc catacgcgcg gcggccaccg gactgtgttt taatatcggc cggtttttca
    31441 cggccaccgc ggtgtttttt gtaggttcat tggtgacctt gttcggagga tttggcaacg
    31501 cccttctggt attctcgatc actttcgtca tcgccttcct cgccgtttat tttaccaggg
    31561 cagaaccgga gcgacagcta cccattcccc aaaccgtgcc ctgatgcttc catttaatta
    31621 tcttaattcc tttaatttct ttaattgtaa aaacataatg cctcatatcc cgcttcccga
    31681 aggaatcccc ggcatccgca gcctggccct gcaatatcct gaatcgggaa aataccttta
    31741 tgagctggtc cagatcttgc tgcggcatga atccccgctg agcgaagctg aaagggaact
    31801 catcgcagcc tacgtctcct accttaatga atgtgaattt tgttgcaaaa gtcacgccgc
    31861 cgcctcaaga tacctcttcg ccgatgcctc gggcacggtg gacatggtgt tgcacgatta
    31921 caggtctgct ccgatttctg ataaattgaa gtccctgctt tccattgccg gcaaagttca
    31981 agccgatgcc cgcaccgtga ccgaggagga tgtggccctg gccagatcct tcggagccgt
    32041 agaccgggac atccacgata cggtactgat cgctgctacg ttttgtatgt tcaaccggta
    32101 tgtggatggc ctggcaaccc tgacgccaga agattctcag gtctatgcgg aaatggggga
    32161 gcggatggcg acaaagggtt atgtagttcc tgaacaaaaa taatcatggc ttatatcccc
    32221 ttagatgaat ccattcccgg aatccggagc ctcctcgcgt attttcctgc catcgaacct
    32281 gcaatgaagg gcttgatgca aaccatgatg cgccaggatc gggggctgaa caaaggtgag
    32341 agggaactca ttgcagcttg ggtatcgcat cacaatcatt gtactgcctg tgaaaacatc
    32401 catggcgccg ttgcctccca tctgcttggc tgggatgaag cggactatgc aaaggttgat
    32461 gaaagaaaag gcaggtggat cacccaggtt ggaggcgctg ctggtcctcg cccggcaagt
    32521 gacctggaat gggaaaaaag tgacttctga acaaataaca aaagccaaag ctttaggatg
    32581 cacggaccag gaagtccacg ataccatatt gatcgcgtcc atgttctgca tgtttaaccg
    32641 ttatattgat ggactgggcc tgaccagccg ggacaccccc gaaagcctta aaacaagggc
    32701 tcaacatctc gcagaatatg gatatggaac ttaagggtaa tccgactttt ataaactcaa
    32761 acacggacca tgtctttcat agatttaaaa aatgatttac cgggaatacg cagcctgatg
    32821 gcatatagtc ctcataccgc caaacccctg aatgaactgg cggaagtcct gcttcgcgat
    32881 gaacacaata ctctgagccg gggggagcgg gagttaatcg gtgcttacgt atcctggctc
    32941 aaccaatgtt ttttttgtca gcaggtccat gaggcgctcg cgggacatta cctggcatgt
    33001 cccattggtg aactcgatca aatatttgat aacctcggcg cgtccgctct gcctgccaaa
    33061 ataaaggcgc ttttattcat agccggccag gtacaaaccg gcggaaaatc cgtaagccag
    33121 gaccaggtgg atgcggccag gcatgccggc gcttccgacc gggaaatcca cgataccatt
    33181 ctcatcgccg ccgcattctg catgttcaac cggtatgtgg atggtcttgg aacctgggca
    33241 cccgccgaca gggggtatta tgtcaaccgg gcgccccaac gcgcaataga aggatatatt
    33301 gactttgacc tttacaagta atcattcatg aataaattat tttggcttgg cttggcgggt
    33361 tggatcctgt ttgaaagttc caacgtctat tttatcatgc ccatgcctgg cagccagcaa
    33421 atgaacagcc tggatctggc ttatttttta tacacctgga gatggttgtt ccgcgctttg
    33481 tttgctggac tgatcctgtt ggggttccgg catgtccact ggaagaaaaa atggcatatg
    33541 gcactgccct ttgcagcgct cctgggggtt atttacttgt ttaatttcaa catggccgcc
    33601 gaccatatgt tttaccagcc atccagggta atctttaaaa acgcgagcca aaatgaagta
    33661 gattccaacc gcctggtcat tggacttacg catcaggggg aagctaaagc ctaccccgtc
    33721 cggttcatgg gttaccacca ccaactgctc gataccgtcg gaggcaaagc cgtcctggtg
    33781 acctattgca ccgtgtgcag gaccggccgg gtgtacgagc cgcgggtcaa cgggaaaccg
    33841 gaaacctttc gtttggtggg catggaccat tttaacgcca tgctcgaaga caaaaccacc
    33901 aaaagctggt ggcgccaggt caacggggaa gccgttgccg gcaaactcaa aggatccatg
    33961 ctgcccgaag tattcagcat acaatgcagg ctcgaagaat ggataaaact ccatccccac
    34021 acgctcatca tgcaggcgga cagcacctac atcgattctt atccggacaa ttataagttt
    34081 gagtccggta aaagcaagag caaactcacc ggtaccgaca gcctttcctg gaaccgaaaa
    34141 agctgggtgg ttggcatccg aactccgcag gccagcaaag cctatgactg gaaccgttta
    34201 aaatcggaat ggattataca cgacgaagtc cgagggactc cgatcctgct ggtcctggcc
    34261 tccgatacgg ccagcttttt tgcttttgaa agacctggtc cggatgcaag gtttaccctg
    34321 gtgaaggata ccctctacct gggtcaacac cgttttgacc tcaaagggag gggactggac
    34381 accaccctgc acctgaagcc cctccaggct tatcaggaat tctggcattc ctgggagacg
    34441 tttaatccgg gcaccctgag gtagggaggt cgggagtatt tccctttttc ggtttagccc
    34501 cgag
//
LOCUS       DAOQXT010000043        34478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1460_length_34478_cov_6.075879, whole genome shotgun sequence.
ACCESSION   DAOQXT010000043 DAOQXT010000000
VERSION     DAOQXT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 34478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34478
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1460_length_34478_cov_6.075879"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PKM27_09345"
     CDS             complement(<1..858)
                     /locus_tag="PKM27_09345"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HNR07508.1"
                     /translation="MNSKLAAQRIIRQGLSHPQFGDPVSVVKHMAGIQAQDFEMAKWA
                     VGIRMQNPDLEAVNAALDSAKIIRAHIFRPTWHLVPGEDYWWMNRFSAPFLRKGHTGR
                     FQQLGLKETLLQKTRKLIHKFLFARPSLTRQQILEVFQSHKFKPDPQQLNHILYDAEL
                     EGLICSGPQQGKTHSYTLVEQRLPTPVGKTAEEALAELTMRYFKSHGPATLQDLTKWM
                     NIPVNTARLGIEMNRSSLDFQTINGKVHWFTDHPEPARSRPRPFLLLPAYDEYLIGYA
                     DRSFAIQPEF"
     gene            895..1272
                     /locus_tag="PKM27_09350"
     CDS             895..1272
                     /locus_tag="PKM27_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR07509.1"
                     /translation="MMASSLLGLRTVIYKVPELEKAKTWYSKAFETKPYFDEAFYVGF
                     NIGGYELGLDPDGYTGDQNKANAVAYWGTENIQETFDRFIQLGATPVEIPHNVGGEIM
                     VSTVADPWGNRIGLIYNPEFKLD"
     gene            complement(1281..2327)
                     /locus_tag="PKM27_09355"
     CDS             complement(1281..2327)
                     /locus_tag="PKM27_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNR07510.1"
                     /translation="MKRRYWPIILLIPVIFFADGLYAGNPDRQGEAGAYELTMNPWAR
                     AAGLHTMTTSMISGVEALWLNPAGLARINRTELLIGHTQYLRGSGISMNALGFAQKMG
                     KNGVLGFNLVSLDFGDILVTTTQLPGGTGATYSPSFFNLGVSYAYTFENKVSVGILLR
                     GISESTSDLSAFGFAIDAGVQYVNGPEDNFKFGISLRNVGSPMRFGGEGLSFQGDNPE
                     GETPYQLTFDQRSAGFELPSLLNIGTSYDFHLDKRNRLTVLANFTANSFSRDELGAGV
                     EYAFNNIFFLRTGYKYELEGVDNKNVYTGLSAGFGIEAPFKKGSNQKLGIDYGYRASN
                     PWNGSHNISLKISL"
     gene            complement(2340..6296)
                     /locus_tag="PKM27_09360"
     CDS             complement(2340..6296)
                     /locus_tag="PKM27_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07511.1"
                     /translation="MFQKKYICSVVLVVFGLVTVWAYPPYPPKKAKLDELKQRLTPRA
                     DCAPSTSSIDMQINNVRARLLGGGDVWWDLQDGKYIVPKVQPGSGIPEVSSIFAGAVW
                     LGGYDPVGNLKLAGQIYRQGNLNDFWPGPLDPVKGTIGIDTCAKWDKHFQVTGENIAA
                     HLKAFNEAQASGTVLDCKKIPEDVLGWPGRGNPYFKNIHGFELPNTSQGLAGFYDRDG
                     DGLYDPCKGDYPIIEIRGCNAPQYPDEMTFWIYNDAGGIHTQTQGKPIQMEIQVQAFA
                     YATNDEINNMTFQRYKLINRATQSIDSTFFAMFVDPDLGCYTDDYIGCDVSRSLAYVY
                     NEDATDGETGCSCPGGINTYCNDVPILGVDYFRGPLDEFGQEIGMSSFTYFNNAAVGN
                     WPNAMTDPQNAQEYYNYMSGSWKDGTPFSYGGTAYQIGAARNDYAFTDAPDNARGWSM
                     CTANLDFGDRRTIQASGPFRLDPGAVNELIIGVVWVPDLDYPCPNIDRLTSADDIAQA
                     LFDNCFDITDGPDAPDLDFIELDQELILLLSNDPVKSNNAYEGYQEVDLQAPAGIADS
                     LYRFEGYKIYQLKSADVGIADIEDVNRARLIFQVDIKNGVRNVYNWKSVPDPNSTQPV
                     WIPEIRVEGEDAGIRHSFQIREDQFATEDRALINHKKYYYTAIAYAYNNYLPFNPRDV
                     VGQRKSYLEGRRNIRTYVPIPRPVVNTNLKSAYGNGVEITRLDGIGAGGSFLDMADST
                     RQAILEKKFDGRIKYKPGRGPIQVKIYNPLDVQDGEFILTFTDADPNNNRLDPPVRWK
                     LINSKDPNTVILSETTLDNLNEQLIAKFGFSVTLGQSIDAGDDPLGNNGAIGAEISYK
                     DLNKPQWFGTVPDGDGVINYVKTGKNEIDFLLDQKSALSTMGNGGGFVPFYLTDFRVS
                     DPLNPFIGPSWMDASDGFRIMRENPGFRLQNLNNVDIVFTPDKSLWSRCVVVETASPF
                     YYSQTTGLGIPTFMDSRNMDLRRSPSVGKEDTNNDDLPDPDNDGTGMGWFPGYAIDVE
                     SGKRLNIFFGENSVYDGRLFPQGFDDQKPIGGDMMWNPTSQQVIQTGGPPTLFNYLLG
                     GHHFVYVTLQEYDECKALRERLNSGNSPLRKIPGLRDVTWTLWPILPTGQKLLSYRDG
                     LIPNETIIKMRVENPFQVAVGTNSAKGHPSYLFTISGQAAQPLQGVEIPAALKKINLV
                     PNPYYAYSSYESTQFANIVKITNLPAKCVVTIYSLDGRFIRQYKRDEKDLPIRDRTNP
                     AVLNTRYIPDLEWDMKNSKGIPVASGVYLVHIQAEGIGETTIKWFNVNRKFDPTGL"
     gene            complement(6307..9918)
                     /locus_tag="PKM27_09365"
     CDS             complement(6307..9918)
                     /locus_tag="PKM27_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNR07512.1"
                     /translation="MHFRKPGITLLLLFFGFLATQAQTSLEGKVEDENKEGLIGATVV
                     LFKSGVQKGGTTTDLDGNYTFTNLDPGTYDVEFSYVGYQTQRIQNVVVFAGKANRLDV
                     EMATGILLNEVVVVDYKVPLIQQDNTTSGGTVTSEQIRNLPTKNINELAATTAGIAQQ
                     DGGALNIRGSRSNATNYYIDGIRVNTSLIPQTEIDQMQVITGGVEARYGDVTGGVISI
                     TTKGPSDKFTGGLELESSQFLDSYGYNLLNFNLSGPILRNQKGVSVLGYRLSGQYRRQ
                     QDDNPGITGTYYTHSGKLKELEAGPITRVNGVPLSSAEFLGSGDVELRKARMNEQASR
                     IDLTGKIDARLTKKIDIGFTGAYNRNNNRFSAGSTWDLLNYENNPLATSQNYRGAFRW
                     RHRLGPDPLENPQGGPRKKGDNRIFRNAVYTLQAAYEKGNNQNEDYRHRDRLFNYGHI
                     GNFDISWIPVEGESTYSRGPIPGVAHAGYLQQLNGYTPGTLNEVLANYNKNLDANNLL
                     DYYTFNGFISNNFNSAWRLHTNVGAVYNNFGKGESDILTLNGEASFDLFPGGSNKGRH
                     NIQAGFIFEQRTDRAWGISPSGLWTIARLQANNHIKGVDTSKVIGSFDGLQTPYTYEQ
                     FQTLIEEDNDLLFYKRIRQLTGQTLNEYVNVDGINPADLRLDMFAASELTDQGVLDYY
                     GYDYLGNKLSGKARFEDFFTAVDAEGRRTFVVAPNRPTYAAAYIQDKFSYRDLILRLG
                     LRVDRYDANTKVLRDPFSLYEITQAKEFYASLGEEKPAGVGDDYLVYITNKNSNNVKA
                     FRKGEQWYFPNGTPANDGSVLFGGEVVTPKLKDPNSNIRLKSFNPDQSFTDYVPQINF
                     MPRLAFSFPISDDANFFAHYDILVQRPPSNTIATALDYYYFEDPARTPSNNPNLKPER
                     TIDYEVGFQQKLTNSSALKIAAYYKEMRDMIQSRTYLYVPSPVNNYTGYGNLDFGTVK
                     GFSFQYDLRRTGNIELLANYTLQFADGTGSNANTQRSLNQRGNIRSLFPLNFDERHRL
                     VTVIDYRYASGRAYNGPRINGLELFANAGINLQANAVSGRPYTKLQRAQPLGGTGFAG
                     ALNGSRLPWSFTLDLRADKSFRVGSPDSRFPMFLNVYLRVQNVLNRRNVYGVYPVTGS
                     PEDDGYLTSTDGKSALNTVTSTGRDVNAYLLSYQWAELNPGFYSLPRRIFLGAIVEF"
     gene            complement(10123..12687)
                     /locus_tag="PKM27_09370"
     CDS             complement(10123..12687)
                     /locus_tag="PKM27_09370"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNR07513.1"
                     /translation="MSFTSGQIEVFNKEVLLKYQLFNSLFTSLPFQKIEKTGILLSLL
                     VTYCEEGYAKGNSPKEIIDHFFRTQTDYIGEKDRLDLLFRFIQYGERQVVLFDALEDA
                     AFALTHDLEGIGTLKQIETETRVKALTGELADRLREFAVRLVLTAHPTQFYPDSVLGI
                     IHELAQCIATNQITEINLLLQQLGKTPFFKHTRPSPVDEANSLIWYLKKIFYPAIGRI
                     NQRVIGMIPETAREQDPLIHLGFWPGGDRDGNPFVTWQTTVEVAGNLRSNLLQCYLDD
                     IRDLKKRLTFKNVDVLIARLEKMILQLAGTNGNVVSKEKILDLLFQVRNIITNEHDSL
                     FLENVDAFIAKVQSFGFYFASLDIRQDSSVHQSVVQAILPFTDHSNVDYPALDEAQKN
                     RFLTTQADLHPDFHLEPGSLEEDTILTIRAMKSIQEQNGELACHRYILSQCESVSHIL
                     EAFFLIRTTWAQPQISVDIVPLFEKIDDLKLASQIMSQLYRDPVYREHLKSRSDFQTI
                     MLGFSDGTKDGGYLMANWSIYQAKENLTAVSKEFGIKVIFFDGRGGPPSRGGGKTHKF
                     YASLGHRISNTEIQLTIQGQTVSSSFGTIASAQYNIEQLLNAGIYNDVLADHTNTLNP
                     DHRQLIDEMATTGMEAFRSLKSHPRFMSYLSTVSPLNYYSETNIASRPSKRGKSKDLR
                     LEDLRAIPFVGSWSQNKQNVPGFFGVGSALEKMYLAGKWDQVRDLYQSSLFFKTLIEN
                     NEMAMKKSFFPLTAYLETDAEFAGLFRIIYDEFKRTQKYVLLLTGHKFLMEDYPADSI
                     SVDMRERIVRPLLTIQQFALMQLRKPEYQDHELRPVYEKLVIRSSFGIINAARNSV"
     gene            12873..14492
                     /locus_tag="PKM27_09375"
     CDS             12873..14492
                     /locus_tag="PKM27_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNR07514.1"
                     /translation="MNSSLVTLDWIVIVGYFFVLVGITIWAMRFSQKDTEDYFLAGRN
                     VGWFMIGASIFASNIGAEHVVGLAGTGANSGMPMAHYELHAWIVLLLGWVFLPFYMRS
                     AVFTMPEFLEKRFSPKARWFLSIFSLVSYVMTKVAVNLYAGGIVTSSLLGIDFWTGAI
                     GVLVLTGIYTVLGGMRAVVLTETFQTIILILGAAFVTILGLQKVGGWGTLTELVGATH
                     FNMWRPSSDPNFPWTGLVFGGAIVGTWYWCTDQYIVQRTLSAKNLTEGRRGAIFGAFL
                     KITPVFIFLMPGIICYALAQQGKITLNNPNEAFTVIMQNLLPAGFRGLVAAGLLAALM
                     SSLASIFNSCSTLFTVDVYKKLRPETSEKNLVRVGRIATGVVVVAGLLWVPILTKIGG
                     GVLYQYLQSVQSYIAPPITAVFLLGIFFKRINSKGAMAALWFGVIFAAVRIIAEINKD
                     SLTGFMHSFASYNFMHLSIWLFLCCVLVAVGVSLLTEKPAEESLKGLTFSTLTPEQKL
                     ATRNSFNKWDVFFSLAILAIVILIMSYFTGN"
     gene            14632..15405
                     /locus_tag="PKM27_09380"
     CDS             14632..15405
                     /locus_tag="PKM27_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNR07515.1"
                     /translation="MSFTIKLKQFEGPFDLLLFFIERDELDIYDIPIAKITADFLDYI
                     HQLEQLNIDLASEFILVAATLMRIKAKMLLPRKEIDEEGNEIDPRQELVQRLLEYKRY
                     KSVLEEFEALELKRLQQASRGNVNQELKKFAEKAFIEAEWESVTLFKLMKTFERMLVQ
                     FDDRRHVHKVYNFIYTVLDQQDYILELVAEREKVSFEDIFVPCENRIHAIITFLALLE
                     LLNQQQVSISSGEGANNFWIGKNHEAVPQQNPGTGDLTL"
     gene            15402..16322
                     /locus_tag="PKM27_09385"
     CDS             15402..16322
                     /locus_tag="PKM27_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HNR07516.1"
                     /translation="MIQYNPKDWFTFIFRFHKADTFRKLLPLLAGMAVYSTLIIYLEI
                     RLSWVNDLHVQNIIQMHSLLGFAISIMLVFRTNTAYDRWWEARRWWGTLVNNSRNLAI
                     KLSTYIPEEDQKLRLRFSKAIGAFALALHKHLRKETIRMELFELDEDDPVMKLHDQDR
                     HVPAQLTELIARIVNRMRKEHTISPEQFLALNNELSNFMEICGACERIKNTPIPFSYS
                     GFIKKFVFFYVMTLPFGFGKSLGYIGVPVVVFIFYVLASLELIAEEIEDPFSGDENDL
                     PTEKLAEVIREHVKIILERGSSQQVGKLAC"
     gene            16499..17185
                     /locus_tag="PKM27_09390"
                     /pseudo
     CDS             16499..17185
                     /locus_tag="PKM27_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
     gene            complement(17294..17980)
                     /locus_tag="PKM27_09395"
                     /pseudo
     CDS             complement(17294..17980)
                     /locus_tag="PKM27_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
     gene            complement(18157..19077)
                     /locus_tag="PKM27_09400"
     CDS             complement(18157..19077)
                     /locus_tag="PKM27_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HNR07517.1"
                     /translation="MIQYNPKDWFTFIFRFHKADTFRKLLPLLAGMAVYSTLIIYLEI
                     RLSWVNDLHVQNIIQMHSLLGFAISIMLVFRTNTAYDRWWEARRWWGTLVNNSRNLAI
                     KLSTYIPEEDQKLRLRFSKAIGAFALALHKHLRKETIRMELFELDEDDPVMKLHDQDR
                     HVPAQLTELIARIVNRMRKEHTISPEQFLALNNELSNFMEICGACERIKNTPIPFSYS
                     GFIKKFVFFYVMTLPFGFGKSLGYIGVPVVVFIFYVLASLELIAEEIEDPFSGDENDL
                     PTEKLAEVIREHVKIILERGSSQQVGKLAC"
     gene            complement(19074..19847)
                     /locus_tag="PKM27_09405"
     CDS             complement(19074..19847)
                     /locus_tag="PKM27_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNR07518.1"
                     /translation="MSFTIKLKQFEGPFDLLLFFIERDELDIYDIPIAKITADFLDYI
                     HQLEQLNIDLASEFILVAATLMRIKAKMLLPRKEIDEEGNEIDPRQELVQRLLEYKRY
                     KSVLEEFEALELKRLQQASRGNVNQELKKFAEKAFIEAEWESVTLFKLMKTFERMLVQ
                     FDDRRHVHKVYNFIYTVLDQQDYILELVAEREKVSFEDIFVPCENRIHAIITFLALLE
                     LLNQQQVSISSGEGANNFWIGKNHEAVPQQNPGTGDLTL"
     gene            complement(19987..21606)
                     /locus_tag="PKM27_09410"
     CDS             complement(19987..21606)
                     /locus_tag="PKM27_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNR07519.1"
                     /translation="MNSSLVTLDWIVIVGYFFVLVGITIWAMRFSQKDTEDYFLAGRN
                     VGWFMIGASIFASNIGAEHVVGLAGTGANSGMPMAHYELHAWIVLLLGWVFLPFYMRS
                     AVFTMPEFLEKRFSPKARWFLSIFSLVSYVMTKVAVNLYAGGIVTSSLLGIDFWTGAI
                     GVLVLTGIYTVLGGMRAVVLTETFQTIILILGAAFVTILGLQKVGGWGTLTELVGATH
                     FNMWRPSSDPNFPWTGLVFGGAIVGTWYWCTDQYIVQRTLSAKNLTEGRRGAIFGAFL
                     KITPVFIFLMPGIICYALAQQGKITLNNPNEAFTVIMQNLLPAGFRGLVAAGLLAALM
                     SSLASIFNSCSTLFTVDVYKKLRPETSEKNLVRVGRIATGVVVVAGLLWVPILTKIGG
                     GVLYQYLQSVQSYIAPPITAVFLLGIFFKRINSKGAMAALWFGVIFAAVRIIAEINKD
                     SLTGFMHSFASYNFMHLSIWLFLCCVLVAVGVSLLTEKPAEESLKGLTFSTLTPEQKL
                     ATRNSFNKWDVFFSLAILAIVILIMSYFTGN"
     gene            21792..24356
                     /locus_tag="PKM27_09415"
     CDS             21792..24356
                     /locus_tag="PKM27_09415"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNR07520.1"
                     /translation="MSFTSGQIEVFNKEVLLKYQLFNSLFTSLPFQKIEKTGILLSLL
                     VTYCEEGYAKGNSPKEIIDHFFRTQTDYIGEKDRLDLLFRFIQYGERQVVLFDALEDA
                     AFALTHDLEGIGTLKQIETETRVKALTGELADRLREFAVRLVLTAHPTQFYPDSVLGI
                     IHELAQCIATNQITEINLLLQQLGKTPFFKHTRPSPVDEANSLIWYLKKIFYPAIGRI
                     NQRVIGMIPETAREQDPLIHLGFWPGGDRDGNPFVTWQTTVEVAGNLRSNLLQCYLDD
                     IRDLKKRLTFKNVDVLIARLEKMILQLAGTNGNVVSKEKILDLLFQVRNIITNEHDSL
                     FLENVDAFIAKVQSFGFYFASLDIRQDSSVHQSVVQAILPFTDHSNVDYPALDEAQKN
                     RFLTTQADLHPDFHLEPGSLEEDTILTIRAMKSIQEQNGELACHRYILSQCESVSHIL
                     EAFFLIRTTWAQPQISVDIVPLFEKIDDLKLASQIMSQLYRDPVYREHLKSRSDFQTI
                     MLGFSDGTKDGGYLMANWSIYQAKENLTAVSKEFGIKVIFFDGRGGPPSRGGGKTHKF
                     YASLGHRISNTEIQLTIQGQTVSSSFGTIASAQYNIEQLLNAGIYNDVLADHTNTLNP
                     DHRQLIDEMATTGMEAFRSLKSHPRFMSYLSTVSPLNYYSETNIASRPSKRGKSKDLR
                     LEDLRAIPFVGSWSQNKQNVPGFFGVGSALEKMYLAGKWDQVRDLYQSSLFFKTLIEN
                     NEMAMKKSFFPLTAYLETDAEFAGLFRIIYDEFKRTQKYVLLLTGHKFLMEDYPADSI
                     SVDMRERIVRPLLTIQQFALMQLRKPEYQDHELRPVYEKLVIRSSFGIINAARNSV"
     gene            24561..28172
                     /locus_tag="PKM27_09420"
     CDS             24561..28172
                     /locus_tag="PKM27_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNR07521.1"
                     /translation="MHFRKPGITLLLLFFGFLATQAQTSLEGKVEDENKEGLIGATVV
                     LFKSGVQKGGTTTDLDGNYTFTNLDPGTYDVEFSYVGYQTQRIQNVVVFAGKANRLDV
                     EMATGILLNEVVVVDYKVPLIQQDNTTSGGTVTSEQIRNLPTKNINELAATTAGIAQQ
                     DGGALNIRGSRSNATNYYIDGIRVNTSLIPQTEIDQMQVITGGVEARYGDVTGGVISI
                     TTKGPSDKFTGGLELESSQFLDSYGYNLLNFNLSGPILRNQKGVSVLGYRLSGQYRRQ
                     QDDNPGITGTYYTHSGKLKELEAGPITRVNGVPLSSAEFLGSGDVELRKARMNEQASR
                     IDLTGKIDARLTKKIDIGFTGAYNRNNNRFSAGSTWDLLNYENNPLATSQNYRGAFRW
                     RHRLGPDPLENPQGGPRKKGDNRIFRNAVYTLQAAYEKGNNQNEDYRHRDRLFNYGHI
                     GNFDISWIPVEGESTYSRGPIPGVAHAGYLQQLNGYTPGTLNEVLANYNKNLDANNLL
                     DYYTFNGFISNNFNSAWRLHTNVGAVYNNFGKGESDILTLNGEASFDLFPGGSNKGRH
                     NIQAGFIFEQRTDRAWGISPSGLWTIARLQANNHIKGVDTSKVIGSFDGLQTPYTYEQ
                     FQTLIEEDNDLLFYKRIRQLTGQTLNEYVNVDGINPADLRLDMFAASELTDQGVLDYY
                     GYDYLGNKLSGKARFEDFFTAVDAEGRRTFVVAPNRPTYAAAYIQDKFSYRDLILRLG
                     LRVDRYDANTKVLRDPFSLYEITQAKEFYASLGEEKPAGVGDDYLVYITNKNSNNVKA
                     FRKGEQWYFPNGTPANDGSVLFGGEVVTPKLKDPNSNIRLKSFNPDQSFTDYVPQINF
                     MPRLAFSFPISDDANFFAHYDILVQRPPSNTIATALDYYYFEDPARTPSNNPNLKPER
                     TIDYEVGFQQKLTNSSALKIAAYYKEMRDMIQSRTYLYVPSPVNNYTGYGNLDFGTVK
                     GFSFQYDLRRTGNIELLANYTLQFADGTGSNANTQRSLNQRGNIRSLFPLNFDERHRL
                     VTVIDYRYASGRAYNGPRINGLELFANAGINLQANAVSGRPYTKLQRAQPLGGTGFAG
                     ALNGSRLPWSFTLDLRADKSFRVGSPDSRFPMFLNVYLRVQNVLNRRNVYGVYPVTGS
                     PEDDGYLTSTDGKSALNTVTSTGRDVNAYLLSYQWAELNPGFYSLPRRIFLGAIVEF"
     gene            28183..32139
                     /locus_tag="PKM27_09425"
     CDS             28183..32139
                     /locus_tag="PKM27_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07522.1"
                     /translation="MFQKKYICSVVLVVFGLVTVWAYPPYPPKKAKLDELKQRLTPRA
                     DCAPSTSSIDMQINNVRARLLGGGDVWWDLQDGKYIVPKVQPGSGIPEVSSIFAGAVW
                     LGGYDPVGNLKLAGQIYRQGNLNDFWPGPLDPVKGTIGIDTCAKWDKHFQVTGENIAA
                     HLKAFNEAQASGTVLDCKKIPEDVLGWPGRGNPYFKNIHGFELPNTSQGLAGFYDRDG
                     DGLYDPCKGDYPIIEIRGCNAPQYPDEMTFWIYNDAGGIHTQTQGKPIQMEIQVQAFA
                     YATNDEINNMTFQRYKLINRATQSIDSTFFAMFVDPDLGCYTDDYIGCDVSRSLAYVY
                     NEDATDGETGCSCPGGINTYCNDVPILGVDYFRGPLDEFGQEIGMSSFTYFNNAAVGN
                     WPNAMTDPQNAQEYYNYMSGSWKDGTPFSYGGTAYQIGAARNDYAFTDAPDNARGWSM
                     CTANLDFGDRRTIQASGPFRLDPGAVNELIIGVVWVPDLDYPCPNIDRLTSADDIAQA
                     LFDNCFDITDGPDAPDLDFIELDQELILLLSNDPVKSNNAYEGYQEVDLQAPAGIADS
                     LYRFEGYKIYQLKSADVGIADIEDVNRARLIFQVDIKNGVRNVYNWKSVPDPNSTQPV
                     WIPEIRVEGEDAGIRHSFQIREDQFATEDRALINHKKYYYTAIAYAYNNYLPFNPRDV
                     VGQRKSYLEGRRNIRTYVPIPRPVVNTNLKSAYGNGVEITRLDGIGAGGSFLDMADST
                     RQAILEKKFDGRIKYKPGRGPIQVKIYNPLDVQDGEFILTFTDADPNNNRLDPPVRWK
                     LINSKDPNTVILSETTLDNLNEQLIAKFGFSVTLGQSIDAGDDPLGNNGAIGAEISYK
                     DLNKPQWFGTVPDGDGVINYVKTGKNEIDFLLDQKSALSTMGNGGGFVPFYLTDFRVS
                     DPLNPFIGPSWMDASDGFRIMRENPGFRLQNLNNVDIVFTPDKSLWSRCVVVETASPF
                     YYSQTTGLGIPTFMDSRNMDLRRSPSVGKEDTNNDDLPDPDNDGTGMGWFPGYAIDVE
                     SGKRLNIFFGENSVYDGRLFPQGFDDQKPIGGDMMWNPTSQQVIQTGGPPTLFNYLLG
                     GHHFVYVTLQEYDECKALRERLNSGNSPLRKIPGLRDVTWTLWPILPTGQKLLSYRDG
                     LIPNETIIKMRVENPFQVAVGTNSAKGHPSYLFTISGQAAQPLQGVEIPAALKKINLV
                     PNPYYAYSSYESTQFANIVKITNLPAKCVVTIYSLDGRFIRQYKRDEKDLPIRDRTNP
                     AVLNTRYIPDLEWDMKNSKGIPVASGVYLVHIQAEGIGETTIKWFNVNRKFDPTGL"
     gene            32152..33198
                     /locus_tag="PKM27_09430"
     CDS             32152..33198
                     /locus_tag="PKM27_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNR07523.1"
                     /translation="MKRRYWPIILLIPVIFFADGLYAGNPDRQGEAGAYELTMNPWAR
                     AAGLHTMTTSMISGVEALWLNPAGLARINRTELLIGHTQYLRGSGISMNALGFAQKMG
                     KNGVLGFNLVSLDFGDILVTTTQLPGGTGATYSPSFFNLGVSYAYTFENKVSVGILLR
                     GISESTSDLSAFGFAIDAGVQYVNGPEDNFKFGISLRNVGSPMRFGGEGLSFQGDNPE
                     GETPYQLTFDQRSAGFELPSLLNIGTSYDFHLDKRNRLTVLANFTANSFSRDELGAGV
                     EYAFNNIFFLRTGYKYELEGVDNKNVYTGLSAGFGIEAPFKKGSNQKLGIDYGYRASN
                     PWNGSHNISLKISL"
     gene            complement(33207..33584)
                     /locus_tag="PKM27_09435"
     CDS             complement(33207..33584)
                     /locus_tag="PKM27_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR07524.1"
                     /translation="MMASSLLGLRTVIYKVPELEKAKTWYSKAFETKPYFDEAFYVGF
                     NIGGYELGLDPDGYTGDQNKANAVAYWGTENIQETFDRFIQLGATPVEIPHNVGGEIM
                     VSTVADPWGNRIGLIYNPEFKLD"
     gene            33621..>34478
                     /locus_tag="PKM27_09440"
     CDS             33621..>34478
                     /locus_tag="PKM27_09440"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HNR07525.1"
                     /translation="MNSKLAAQRIIRQGLSHPQFGDPVSVVKHMAGIQAQDFEMAKWA
                     VGIRMQNPDLEAVNAALDSAKIIRAHIFRPTWHLVPGEDYWWMNRFSAPFLRKGHTGR
                     FQQLGLKETLLQKTRKLIHKFLFARPSLTRQQILEVFQSHKFKPDPQQLNHILYDAEL
                     EGLICSGPQQGKTHSYTLVEQRLPTPVGKTAEEALAELTMRYFKSHGPATLQDLTKWM
                     NIPVNTARLGIEMNRSSLDFQTINGKVHWFTDHPEPARSRPRPFLLLPAYDEYLIGYA
                     DRSFAIQPEF"
BASE COUNT         8454 a         8785 c         8785 g         8454 t
ORIGIN      
        1 aaactccggt tggatggcga agctacggtc agcataacca atcaggtatt catcgtaggc
       61 cggcaagagg agaaaggggc ggggtcggct gcgtgcaggt tcagggtggt ctgtgaacca
      121 atggactttt ccgtttatgg tttgaaagtc aagcgaggac cggttcattt ctatgcccag
      181 ccgggcagtg tttacaggga tattcatcca cttggtgaga tcctgaaggg tagcgggacc
      241 atggctttta aaataccgca tggtcagttc tgccaaagcc tcttccgccg ttttgcccac
      301 cggggtaggc agtctttgct ccaccagcgt ataggaatgc gtctttcctt gttgggggcc
      361 actgcaaata aggccttcca gctctgcgtc gtaaaggata tggttaagtt gctgagggtc
      421 cggtttgaat ttatggcttt gaaaaacttc gaggatttgt tgacgggtga gagagggccg
      481 ggcaaacaaa aatttatgga tcagtttcct ggttttctgc aacagggttt ccttcaatcc
      541 cagttgttga aacctgcccg tatgtccttt tctaagaaat ggtgccgaaa accggttcat
      601 ccaccaataa tcctctcccg ggaccaggtg ccaggtgggc ctgaaaatat gagccctgat
      661 gatctttgcc gaatccaggg cagcattgac ggcctccagg tccgggttct gcatacggat
      721 ccccaccgcc cacttggcca tttcgaaatc ctgggcctgg atcccggcca tatgtttcac
      781 caccgaaacc ggatcgccga attgaggatg ggagaggccc tgccggatta ttcgttgtgc
      841 cgccagctta gaattcatcg ttcatagagt atttttacaa aaaataaaat taatatgatg
      901 gcttcatccc tcctcggact ccgaacggtg atctataaag tgcctgaact ggaaaaggca
      961 aaaacctggt acagcaaggc cttcgaaacc aaaccctatt ttgacgaagc cttttacgtc
     1021 ggattcaata taggaggtta tgaattgggc ctggacccgg atggctatac cggggaccaa
     1081 aacaaggcca atgcagtggc ttactggggc acggaaaata tccaggagac ctttgaccgg
     1141 ttcattcagc ttggtgcgac cccagttgaa atacctcaca acgtaggtgg agaaatcatg
     1201 gtttcgacgg tggccgaccc ctggggaaac cggatcggat tgatttataa tcctgaattc
     1261 aaattggatt aaagcagcta ttacaaggaa attttgagcg atatattgtg gctcccgttc
     1321 cagggattgc tcgcgcggta cccataatct atacccagtt tctgattgct cccttttttg
     1381 aagggagcct caatcccgaa gcctgctgac aaacccgtat agacattttt attgtccacg
     1441 ccttccaact catatttgta accggtgcga agaaagaaaa tgttgttgaa cgcatattcc
     1501 accccggctc caagctcatc ccgtgaaaaa gaattggctg taaaattggc caggaccgtg
     1561 agccggttcc tcttgtccag gtgaaaatca taggaagttc cgatgttaag gagcgagggc
     1621 aattcaaaac ctgcagaccg ctggtcaaag gtcagttgat aaggggtttc cccttccggg
     1681 ttgtcccctt ggaaggacag gccctctccg ccgaaacgca tgggagagcc cacattccgc
     1741 agcgagatcc caaatttaaa attatcctcc gggccgttaa cgtactggac tccggcgtca
     1801 atggcgaatc caaaggcgct caggtcggag gttgattcag aaatgccacg aagcagaata
     1861 cccacggaga ccttgttttc aaaggtatat gcataactga ccccgagatt aaaaaaactg
     1921 ggtgaatagg tcgccccggt tccgcccggc aattgggtgg tggtcaccag gatatctccg
     1981 aaatccaggg aaacaaggtt aaatccgagg accccgtttt tacccatttt ctgtgcaaaa
     2041 cccagggcgt tcatactgat gcccgatccc ctcagatatt gagtatgtcc aataagcaat
     2101 tcagtccggt tgatccgggc caggcctgcc ggattgagcc aaagcgcttc cacaccggag
     2161 atcatagacg tcgtcatggt atggaggccc gcggcccgcg cccatggatt catggtcagt
     2221 tcatatgccc cggcttcccc ctgcctgtcc gggttgcccg catacaatcc atcggcgaaa
     2281 aagatcaccg ggatcaataa aattatcggc cagtatctgc gcttcattga cttcatgctt
     2341 tataaaccgg tgggatcgaa tttgcggttg acgttaaacc atttaatcgt tgtttccccg
     2401 atcccttcgg cctggatgtg gaccaggtaa acgccgctgg cgaccggtat cccttttgaa
     2461 tttttcatat cccattccag gtcggggata taccgggtat tcagcaccgc gggattggtc
     2521 cggtcccgga taggaagatc cttttcatcc cgcttgtact gccggatgaa cctgccatcg
     2581 agggagtaga tggtgactac acatttggca ggcaggttgg tgatctttac aatattggca
     2641 aactgcgtgg attcgtaact ggaatacgcg taatagggat tgggcaccag gtttattttt
     2701 ttaagggcag ccggtatttc aacgccctgc aagggctgtg cggcctgccc cgaaatggta
     2761 aacaggtagg agggatgacc tttggcgctg ttggtgccaa ccgcgacctg gaatgggttt
     2821 tccacccgca tcttaatgat cgtttcattg ggtatgagac cgtccctgta ggacagcagc
     2881 ttttgtcctg tgggcaaaat gggccacaag gtccaggtga cgtcgcgaag tccgggtatt
     2941 ttccgcaaag ggctgttccc ggaattcaaa cgttcgcgca aggccttgca ttcatcgtat
     3001 tcctgcaagg tcacatagac gaagtggtgt ccccccagga gataattaaa aagggtagga
     3061 gggccgccgg tctggatcac ctgctgactg gttggattcc acatcatatc ccctccgatc
     3121 ggtttttgat catcgaagcc ctggggaaac aaccggccgt catacaccga attttctcca
     3181 aaaaagatat tcaacctttt accggattcc acatcgatgg cataacccgg aaaccagccc
     3241 attccggttc cgtcgttgtc cggatcagga aggtcatcgt tgttggtatc ctctttgcca
     3301 accgaagggc ttctgcgcaa atccatattc ctggaatcca taaaggtggg aatgcccagg
     3361 ccggtggttt gggaataata gaaaggggaa gccgtctcca ccaccacgca gcggctccac
     3421 aggcttttat ccggcgtgaa cacgatatcc acgttattta gattctggag ccggaacccc
     3481 gggttttccc gcatgatccg gaaaccatcc gaggcatcca tccaggatgg accgataaaa
     3541 ggattcagag gatccgaaac ccggaaatcg gtgaggtaaa aggggacaaa accgccgcca
     3601 ttgcccatag tggacaaggc ggatttctga tccaggagaa aatcgatctc atttttgcct
     3661 gtcttgacat aattgattac cccgtctccg tccggcaccg tcccaaacca ctgcggttta
     3721 ttgaggtctt tataactgat ctcggctccg atggctccat tgttacccag aggatcatcg
     3781 ccggcatcga tgctttggcc gagcgtgacg gagaagccga atttggcaat caattgttca
     3841 ttgagattat ccagggtggt ctccgagagg atgaccgtat tcgggtcctt gctgtttatc
     3901 agtttccatc tgaccggcgg gtcgagccgg ttattgttgg ggtctgcatc ggtaaaggtg
     3961 aggatgaatt caccatcctg cacgtccagc ggattataga tcttcacctg gatcggaccg
     4021 cggcctggtt tgtatttgat gcggccatca aattttttct cgaggatagc ctggcgggtg
     4081 gaatcggcca tatccaggaa actgccgcct gccccgatgc catccagccg ggtgatctcc
     4141 acgccgttgc cataagcgga cttgagattg gtattcacca cgggccttgg aatgggaaca
     4201 taagtacgga tattcctcct gccctccagg tatgattttc gctggccgac gacatccctg
     4261 ggattaaaag gcaggtaatt gttataggca taagcgatgg ccgtataata atacttttta
     4321 tggttgatca gggcccggtc ctcggtggca aactggtctt ccctgatctg gaaactgtgc
     4381 ctgatcccgg catcttctcc ttccacccgg atttccggaa tccaaaccgg ctgggtggaa
     4441 ttggggtctg gcaccgattt ccagttatat acattccgca ccccgttttt gatatccacc
     4501 tggaagataa gccttgcccg gttgacgtcc tcaatatccg cgatgccgac atcggcactt
     4561 ttgagctggt aaattttata tccttcaaaa cgatagaggc tgtcggcaat cccggcgggg
     4621 gcttgcagat ccacttcctg gtatccttca taggcattat tcgatttcac cgggtcattg
     4681 gacaaaagca aaatcagttc ctggtcgagt tctataaaat cgaggtccgg tgcgtccggt
     4741 ccgtcggtga tgtcaaaaca attgtcaaac aaggcctgcg cgatgtcatc ggctgaagtc
     4801 agccggtcga tattcgggca gggataatcc aaatcgggca cccaaaccac accaatgatg
     4861 agttcattga ccgcaccggg atccagccgg aaaggacctg aggcctggat ggtccggcgg
     4921 tctccaaagt ccaggtttgc ggtacacatg gaccagccgc gggcgttgtc cggggcgtcg
     4981 gtaaaggcat aatcatttct ggcagccccg atctgataag ccgtgcctcc gtaagaaaag
     5041 ggcgtgccgt ctttccagga tccggacatg taattatagt attcctgcgc gttttgcgga
     5101 tcggtcatgg cgttgggcca gttgccgact gcagcattgt taaaataagt gaaggaggac
     5161 atgccgattt cctggccgaa ttcatccagg gggcccctga aataatccac gccgagaatg
     5221 ggcacatcgt tgcaataggt gtttatgcct cccgggcagg agcatcccgt ttctccgtcg
     5281 gtggcatctt cgttgtacac ataggccaaa gacctgctca catcgcatcc gatgtagtcg
     5341 tctgtataac aacccaggtc agggtccaca aacatcgcaa agaaggtact gtcgatgctt
     5401 tgggtggcgc ggttgatcaa tttataccgc tggaaggtca tattgttgat ctcatcgttg
     5461 gtcgcatagg caaaggcctg gacctggatt tccatctgga tgggtttacc ctgggtctgg
     5521 gtgtgtatcc ccccggcgtc attatagatc caaaaggtca tttcatcggg atactggggg
     5581 gcgttgcatc cacggatctc gatgatggga tagtcccctt tgcaggggtc gtataatccg
     5641 tcgccgtcgc ggtcataaaa gccggcaagc ccctggctgg tattggggag ctcaaagccg
     5701 tggatgtttt taaagtaagg attgcccctg ccgggccagc cgaggacgtc ttccggtatt
     5761 tttttgcaat ccagcacagt ccctgaagcc tgggcttcgt taaacgcttt caggtgggcg
     5821 gcgatatttt cacccgtgac ctggaaatgt ttgtcccatt tggcacaggt gtcaatgcct
     5881 atggtgcctt ttaccggatc gagagggccc ggccaaaaat cgttgagatt tccctggcgg
     5941 taaatctggc cggcaagttt gaggttgccc accggatcgt atccgcccag ccagaccgcg
     6001 ccggcgaata tggaggatac ctcagggatt cccgatccgg gttgcacctt tggaacaatg
     6061 tatttaccat cctgcaggtc ccaccagaca tcccctccgc ccaaaagcct ggcccttacg
     6121 ttattgattt gcatgtctat ggaggaggtg gaaggggcac aatcggcccg gggggtaagc
     6181 ctttgtttga gctcatccag ctttgccttt tttggcgggt agggtggata agcccatacc
     6241 gtaaccaggc cgaacaccac caggaccaca gagcatatat atttcttttg aaacatcatc
     6301 atggatttag aattcgacaa tagcgcccag gaaaattctt ctcggaaggg aataaaatcc
     6361 gggattcaat tctgcccact ggtaggacaa taaataagcg ttgacatccc tgccggtgga
     6421 agtcaccgtg ttgagcgccg acttgccatc cgtggaggtc aggtagccgt catcctccgg
     6481 cgatccggtg acgggataga ccccatagac gttcctgcgg ttcaatacat tctgcacccg
     6541 caggtaaaca ttcagaaaca tgggaaaacg gctgtccgga ctgcccaccc tgaaactttt
     6601 atctgcacgc agatccagcg tgaaggacca gggaagccgg gaaccgttga gggcgcctgc
     6661 aaaacccgtc ccgcccaggg gctgcgccct ttgcaatttg gtatagggcc tgccgctgac
     6721 cgcattggcc tgtaagttga taccggcatt ggcaaagagt tcaagcccgt tgatccttgg
     6781 cccgttgtac gcccttccgg aagcataacg gtaatcgatc acggtgacca gccggtgcct
     6841 ctcatcaaaa ttcagcggaa aaagactcct gatgtttccc cgttgattca ggctgcgctg
     6901 ggtattggcg ttggagccgg ttccgtccgc aaactggagg gtgtaattgg ccagcaattc
     6961 aatattgccg gtcctccgga gatcatactg aaaactgaat cctttcacgg tcccgaaatc
     7021 gaggttacca taaccggtat aattatttac gggcgaaggc acatacagat aggtgcggct
     7081 ttggatcata tcgcgcatct ctttgtaata ggctgcgatt ttcagtgccg aagaattcgt
     7141 cagtttttgc tgaaacccga cctcataatc aatggtccgt tcgggtttca ggttggggtt
     7201 gttggaaggg gtcctggccg ggtcttcaaa ataatagtag tccagggcag tggcgatggt
     7261 attggaagga ggcctctgca ccaggatatc ataatgtgca aaaaagttgg catcgtccga
     7321 aatcgggaag gagaaggcca ggcgcggcat aaaattgatc tggggaacgt agtccgtaaa
     7381 ggactggtcc ggattgaagg atttgaggcg gatattggaa ttggggtctt tcagttttgg
     7441 ggtcaccact tccccgccga acaataccga cccgtcgttg gccggcgtgc cgttggggaa
     7501 ataccattgt tccccttttc ggaaggcttt tacgttgttt gagtttttgt tggtgatata
     7561 gaccaggtag tcgtctccaa caccggccgg cttttcttca ccgagagagg cataaaactc
     7621 tttggcctgg gtgatctcat acagggaaaa agggtcccgg agcactttgg tattggcgtc
     7681 ataacggtcg acacgcaggc ccagcctcag gatcaggtcc cggtaactga atttatcctg
     7741 gatataggca gcggcatagg tcggccggtt tggagcgact acaaaggtcc ggcgtccctc
     7801 cgcgtcgacg gccgtgaaaa aatcttcaaa gcgagctttg ccgctcaatt tattccccag
     7861 gtaatcatag ccgtaataat ccagtacgcc ctggtccgta agttcactgg cagcaaacat
     7921 atccagccgc aggtcggccg gattgatgcc gtctacattc acgtattcat tcagggtttg
     7981 tccggtaagt tgcctgatcc ttttataaaa caggaggtca ttgtcttcct cgatcagggt
     8041 ttgaaattgt tcatacgtat agggagtttg cagcccatcg aaagaaccga tgacttttga
     8101 agtgtccaca cctttgatat ggttgttggc ctggagccgg gcaatggtcc agagtccgct
     8161 gggactgatg ccccaggccc tgtcggtgcg ctgttcgaag atgaagccgg cctggatgtt
     8221 atgccttccc ttgttggacc ctccgggaaa cagatcgaag ctggcttcgc cattcagggt
     8281 gaggatatcg ctttctcctt tgccgaagtt gttgtagacg gcgcctacat tggtatgaag
     8341 gcgccaggcc gagttgaaat tgtttgaaat aaaaccgttg aaggtataat aatccaaaag
     8401 gttgttggcg tccaggtttt tattgtaatt ggccagcacc tcgtttaaag tgcccggcgt
     8461 ataaccgttg agttgctgca ggtaacctgc gtgggctacg cccgggatcg ggccgcggga
     8521 gtaggtagac tcaccctcta cggggatcca gctgatatca aaattgccga tatggccgta
     8581 gttaaagaga cggtccctgt gccggtaatc ctcattttga ttattccctt tttcgtaagc
     8641 ggcttgcagg gtatacaccg catttcgaaa aatccggtta tccccttttt tccgcgggcc
     8701 gccctgtggg ttttccaaag ggtcgggacc gaggcggtgc ctccaccgga aggcgccccg
     8761 gtagttttgg gaggtagcca ggggattgtt ttcataattg agcaggtccc aggtggatcc
     8821 ggcagaaaaa cggttgttgt tccggttata ggctccggtg aatccgatgt caattttttt
     8881 ggtgagcctt gcatctattt taccggtgag gtcaatccgg gacgcctgtt cattcatcct
     8941 ggcttttcgc aactccacgt ccccggaacc caggaattcc gcggaggaaa gcggcactcc
     9001 gttgacccgg gtgatcggac cggcttctaa ttctttcagc ttccctgaat gagtgtaata
     9061 ggttcctgta atgccggggt tatcatcctg ctgcctgcgg tattggccgc tgagccggta
     9121 gcctaagacg gaaacgcctt tctggtttct caatatcggg ccgctcaggt tgaaattgag
     9181 caggttgtat ccataagaat ccagaaattg tgagctttcc agttctaaac cgccggtaaa
     9241 tttgtcggag ggtcctttgg tcgtaatgga aatcaccccg ccggtcacat ccccatagcg
     9301 ggcttccacg ccgccggtga tcacctgcat ctggtcgatc tccgtctggg gaatgaggct
     9361 ggtgttgacc cggatgccat caatataata gttagtggca ttggacctgg atcccctgat
     9421 gttcagcgcc cccccgtcct gctgggcgat ccctgcggtg gtggctgcca gttcattgat
     9481 gtttttcgtg ggtaggtttc ttatttgttc ggaggtgacc gtgcccccgg aagtggtatt
     9541 gtcctgctgg attaagggca ctttatagtc taccaccacg acttcattca acaggatccc
     9601 ggttgccatt tccacgtcaa gccggtttgc cttgccggca aaaaccacta cattctgaat
     9661 gcgctgggtt tgatagccca cataactgaa ttccacatca taggtgcccg gatcgagatt
     9721 tgtaaaagta taattcccgt ccaggtcggt cgtcgtaccc cctttctgca ccccgctttt
     9781 aaataatacc accgtggcgc cgatcaggcc ttctttattt tcgtcctcta cctttccttc
     9841 aagggaggtc tgggcctgtg tggccagaaa tccaaaaaag agcaaaagga gggtaatacc
     9901 tggtttccgg aaatgcatag atgaagccaa atagttaaaa ttcaacagaa tagatgatgt
     9961 aaatattcaa cgaagatagg acaagaaacg ctgattcaaa gcgcttttaa agagggcaat
    10021 aaaaatttgc ctcaaagcgc agtcccaaag ctaaacttta ccgagaagag aacttatcca
    10081 aactttccaa cttgcgaacc ttctaactta ctaacttgca aactaaaccg aattccttgc
    10141 tgcattgatg atcccgaatg aactcctgat caccagtttt tcataaaccg gtctgagttc
    10201 atggtcctgg tattccggtt tgcgcaattg catcagggca aattgctgga tggtcagtaa
    10261 ggggcgcacg attctttccc gcatatccac cgaaatggaa tctgccgggt aatcctccat
    10321 caggaatttg tggccggtga ggaggaggac atatttttgg gtccttttga attcatcgta
    10381 aatgatcctg aaaaggccgg caaattcagc atccgtttcc aggtaggcgg tgagggggaa
    10441 aaaactcttt ttcatcgcca tttcattgtt ttcgatcagg gtcttgaaaa agagggaaga
    10501 ttggtacaag tccctgacct ggtcccattt acctgccaaa tacatctttt cgagcgcgga
    10561 gcccactccg aagaagcccg ggacattttg tttgttctgg ctccaggacc ctacgaaagg
    10621 gatggccctc aggtcctcga ggcggaggtc tttggatttt cctcttttgg acggccggct
    10681 ggcaatgttt gtctcactgt agtagttcag ggggcttacg gtgctcaggt aggacatgaa
    10741 cctgggatgc gatttgaggc tgcggaaggc ttccatgcct gtagtggcca tttcgtcaat
    10801 gagttgtcgg tgatccggat ttagggtatt ggtatgatcg gccaacacgt cattgtagat
    10861 cccggcgttc aataattgct cgatattgta ttgcgcggag gcgatggttc caaaactact
    10921 gctgaccgtc tggccctgga tggtcaactg gatctccgta ttggaaatcc ggtgccccag
    10981 ggaggcgtag aatttatgtg tttttcctcc tccgcgcgat gggggcccgc ctcttccgtc
    11041 aaaaaatatc accttgatgc caaactcctt ggacactgcc gtgaggtttt cttttgcctg
    11101 gtaaatgctc cagttggcca taagatatcc tccatccttg gtgccatccg agaatcccag
    11161 catgatggtt tgaaaatcgg accggctttt taaatgttct cggtacaccg ggtcgcggta
    11221 taactgactc atgatctggc tggccagttt aagatcatcg attttttcaa aaaggggtac
    11281 gatgtccaca ctgatctggg gctgtgccca ggtggtccgg atcaggaaga aggcttccag
    11341 gatatggctt accgattcgc attgactaag gatataacga tggcaggcca gttcgccatt
    11401 ctgctcctga atggatttca tggcccggat ggtaaggata gtatcttctt ccagggagcc
    11461 gggttcaaga tggaaatcgg gatggagatc cgcctgggtt gtcaggaacc ggtttttttg
    11521 ggcttcgtcc agcgcagggt agtccacatt ggaatgatcc gtgaacggaa ggattgcctg
    11581 gaccaccgac tgatggaccg aggaatcctg gcggatatcg agggatgcaa aataaaaacc
    11641 aaaggactgg acttttgcga taaaagcatc cacgttttcc aggaaaaggg agtcgtgctc
    11701 attcgtaatg atattccgga cctggaacag gaggtcgagg attttctctt tggagaccac
    11761 attgccattg gtcccggcca gttgaaggat cattttttcc aaccgcgcga tgagtacatc
    11821 cacattttta aaggtcagcc tttttttaag gtcccggata tcgtccaggt agcattgcag
    11881 gaggtttgac ctcaggttgc ctgcgacttc caccgtggtt tgccaggtga caaatggatt
    11941 tccatcgcgg tcgccgccgg gccagaaacc caggtggatg aggggatcct gttcccgggc
    12001 ggtctccggg atcatgccga tgacgcgctg attgatcctt ccgatcgccg gataaaatat
    12061 ttttttaaga taccagatca ggctgttggc ttcatccacg ggagatggcc gcgtatgttt
    12121 gaaaaagggg gttttaccca attgctgaag caagaggttg atttcggtga tttggttggt
    12181 ggcgatacat tgagccagtt cgtgaataat gcccaggacc gaatcgggat aaaactgagt
    12241 agggtgggcg gtaagcacca aacgaacggc gaattccctg agccggtcgg ccaactcgcc
    12301 ggtcagcgcc ttgaccctgg tttccgtttc aatttgtttt aaagtaccga tgccctcgag
    12361 gtcatgggta agggcaaagg ccgcgtcctc cagggcatcg aacaatacca cctgcctttc
    12421 cccatactgg ataaaccgga agagcaggtc cagcctgtct ttttccccga tataatcggt
    12481 ttgagtcctg aaaaaatggt ctatgatttc tttagggctg ttgccttttg catatccttc
    12541 ctcgcaataa gtgaccagca gggacaagag gatacccgtt ttttcgattt tctgaaaggg
    12601 caatgaggta aaaaggctgt tgaacaactg atacttcaat aaaacttcct tgttgaatac
    12661 ttcgatctgg ccggaggtaa aagacatagg ggcaaatatc gatgaatcgg gcgaagcccg
    12721 gtaaggggct tcctaatttt tcactcccgg gctttttgca ttgaaatgac aaatgccctg
    12781 ttttttgaat ttttatatga ccggatgccc ggaatggaga gcagaatttc agagaatttt
    12841 ttcgatatta cgcacccatt aataaaatga atatgaatag cagtttggta accttagact
    12901 ggattgtcat cgttgggtat tttttcgtcc tggtgggcat caccatttgg gcgatgcgat
    12961 tttctcaaaa agatacggag gattatttcc ttgccgggcg caatgtcggc tggtttatga
    13021 tcggggcttc catttttgct tccaatatcg gggccgagca tgtggtgggc ctggccggaa
    13081 caggagcaaa cagcggcatg cccatggcgc actatgaatt gcacgcctgg atcgtgctgt
    13141 tgcttggatg ggtattcctt cctttttaca tgcggagcgc cgtgtttacc atgccggaat
    13201 tcctcgaaaa acggttcagt ccgaaagccc gttggttttt atcgattttt tcactcgtca
    13261 gttatgtcat gaccaaggtg gccgtcaacc tttacgccgg agggatagtg acttcctccc
    13321 tgctcggcat cgatttctgg accggggcga tcggggtcct ggtgctgacg ggaatttata
    13381 cggttttggg gggcatgagg gccgtggtgc ttacggaaac ctttcaaacc atcatcctca
    13441 tcctgggcgc ggcttttgtg acgatcctgg gattgcaaaa ggtcgggggc tggggcaccc
    13501 tcaccgagct ggtcggcgcc acccatttta atatgtggcg gccttcttcc gatccgaatt
    13561 ttccctggac cggactggtt ttcggaggcg ccatcgtggg cacctggtat tggtgtacag
    13621 accaatatat tgtgcaacga accctttccg ccaaaaacct cacggaagga agacgggggg
    13681 ctatcttcgg ggcctttttg aaaatcacgc cggttttcat tttcctgatg cccggcatca
    13741 tttgttatgc gcttgcgcaa cagggaaaaa ttaccctgaa caacccgaat gaagcgttta
    13801 ccgtgatcat gcaaaatctc ctgccggccg gtttcagggg tctcgttgcc gcaggattgc
    13861 tggcagccct gatgagttcg ctggcctcca tattcaacag ttgcagcacg ctcttcacgg
    13921 tcgatgtata taaaaagctg aggccggaga ccagcgagaa aaacctggtc cgggtaggcc
    13981 gcatcgccac cggcgtggtg gtggtcgccg gtttgttgtg ggtccccatt ctgaccaaaa
    14041 tcggcggcgg tgtcctttac cagtatttgc aaagcgtgca gagttatatc gccccgccca
    14101 tcaccgcggt cttcctcctg ggcatatttt ttaaacggat caactcgaaa ggggctatgg
    14161 ctgccttatg gtttggtgtg atttttgcag cggtccgtat tatcgccgaa ataaacaaag
    14221 attctttgac cggatttatg cattcttttg ctagctacaa ttttatgcat ttgagcatct
    14281 ggctcttttt atgctgtgtc ctggtagcgg ttggagtgag cctgcttact gaaaagccgg
    14341 ccgaagaatc acttaaaggt ttgacattca gtacgctgac acccgagcaa aaactggcca
    14401 cgcgaaacag ttttaacaag tgggatgtct ttttttccct tgctatcctg gccattgtca
    14461 tcctgatcat gagctatttt accggcaatt agttaggtat aaaatcaaga cgaatgccgg
    14521 gaaggggatc cgcaagggtc ccctctttta tttttaggaa aaattatttt tctccttatt
    14581 ctgggtattt ttgccccgga aggcatcccc gtataaaaag cattctcctc tatgtctttt
    14641 accattaaac tgaaacaatt tgaaggcccc tttgatcttc ttttattttt tattgaaagg
    14701 gatgaactgg atatatacga tatccccata gccaagatca ccgccgactt cctggattat
    14761 atccaccagc ttgaacaact caacatcgac ctggccagtg aattcatcct ggtggccgcc
    14821 acgctgatgc gcatcaaggc aaaaatgctg ctgcccagga aggaaatcga tgaagaagga
    14881 aacgaaatcg atccccggca ggagctggtg caacgcctgc tggaatacaa gcgctacaag
    14941 agtgtgctcg aagaatttga agccctcgaa ctcaaacgcc tgcaacaggc cagccgggga
    15001 aatgtcaacc aggagttgaa aaagtttgcg gaaaaagctt tcatcgaggc ggaatgggaa
    15061 tccgtaaccc ttttcaaact catgaaaacc ttcgaacgca tgctggtcca gtttgatgac
    15121 cgccggcatg tgcacaaggt ttacaatttc atctatacgg tccttgacca gcaggactac
    15181 atcctggagc tggtcgctga aagagaaaag gtatcctttg aagatatttt tgtcccttgc
    15241 gaaaaccgga ttcacgcgat cattaccttt ctggcccttc tggaattgct caaccaacag
    15301 caggtaagta taagctccgg ggaaggagcc aacaatttct ggatcggcaa aaatcacgaa
    15361 gcggtgcccc aacaaaatcc cggcaccggc gaccttacct tatgattcaa tacaacccga
    15421 aggactggtt taccttcatt ttcagatttc acaaggcgga taccttccgg aagctcctgc
    15481 ccttgctcgc aggcatggcc gtttattcca ctttgatcat ctatctcgaa atccggcttt
    15541 catgggtcaa tgacctccat gtccagaaca tcatccagat gcacagcctt ctgggattcg
    15601 ccatctccat tatgctcgta ttcaggacca acaccgctta tgaccgctgg tgggaagcaa
    15661 gaaggtggtg ggggaccctc gtcaacaaca gccgcaacct ggcaatcaag ctgagtactt
    15721 acatcccgga ggaggaccaa aagctcaggc tcaggtttag caaggcgatt ggagcctttg
    15781 cactcgcctt gcataagcat ctccggaaag aaaccatccg gatggagctg tttgaactgg
    15841 atgaagatga ccctgtcatg aagctgcacg accaggacag gcatgtaccc gcacaactga
    15901 cggagctcat cgcccgcatc gtcaaccgga tgcgcaagga acatacgatc tctccggaac
    15961 aattcctggc cctcaacaac gaattgtcca attttatgga aatctgcggg gcctgcgaaa
    16021 ggatcaaaaa caccccgatt cctttttcct acagcggatt tattaaaaaa ttcgtctttt
    16081 tttatgtgat gaccctccct tttggatttg gaaagagcct gggttatata ggtgtgccgg
    16141 tggtggtttt tatcttttat gtactggcca gcctggagct catcgcggaa gagattgaag
    16201 acccctttag cggggacgaa aacgaccttc ccactgagaa actggcagaa gtcatccgtg
    16261 aacatgtcaa gattatctta gagcggggta gtagtcagca agttggcaag ttggcatgtt
    16321 agtaagttgg taagttggta agttggctca cagctgaaag ctgaaagctg atagctgata
    16381 gctgataact gaatgctgaa tgctgaatcc ggttttgaaa tcccgaattc tggccttatc
    16441 tttgcgtcgc ttttcaataa aatagttaca ataagctcat tttcaaagaa taaaatatat
    16501 ggcaaatatc ggtaaagtca aacaggtaat cggtccggtg gtggatatca gtttcgaagg
    16561 accggattcc accctgcctg caattctcag cgccctggtg gtccggaaaa agaacgggaa
    16621 gaacctcatc ctggaagtcc agcaacacct cggtgaagac accgtaaggg ccattgcgat
    16681 ggattcgacg gatggcctca tgcggggtat tgaagtggaa gataccggtg ccaatatgac
    16741 catgccaacc ggggatgtca tcaaagggag gttgtttaat gtggtgggtg aagccatcga
    16801 cggcattggg ccggtaatta aagataagaa tgcctatccc atacaccgta agccgccggc
    16861 ctacgagcaa ctgtccaccg aaaaagaaat tctgtatacc gggatcaagg tcatagacct
    16921 gatcgaacct tatatgaaag gggggaaaat tggtttgttt gggggtgccg gtgttggcaa
    16981 gaccgttctg atcatggaac tgatcaacaa catcgccaaa gcctatgaag gcatttcggt
    17041 gtttgccggg gtgggagagc ggacccgtga aggcaacgac ctgctgcgcg agatgattga
    17101 atccggggtt attaaatacg gggaggaatt caaacacagt atggagaaag gcggatggga
    17161 cctcagcaag gtcgatccca aagcgctgga gacttcccag gccaccctgg ttttcggcca
    17221 gatgaatgag cccccgggcg cccgggggct cattcatctg gccgaaaacc agggtggcct
    17281 gggaagtctc cagcgctttg ggatcgacct tgctgaggtc ccatccgcct ttctccatac
    17341 tgtgtttgaa ttcctccccg tatttaataa ccccggattc aatcatctcg cgcagcaggt
    17401 cgttgccttc acgggtccgc tctcccaccc cggcaaacac cgaaatgcct tcataggctt
    17461 tggcgatgtt gttgatcagt tccatgatca gaacggtctt gccaacaccg gcacccccaa
    17521 acaaaccaat tttcccccct ttcatataag gttcgatcag gtctatgacc ttgatcccgg
    17581 tatacagaat ttctttttcg gtggacagtt gctcgtaggc cggcggctta cggtgtatgg
    17641 gataggcatt cttatcttta attaccggcc caatgccgtc gatggcttca cccaccacat
    17701 taaacaacct ccctttgatg acatccccgg ttggcatggt catattggca ccggtatctt
    17761 ccacttcaat accccgcatg aggccatccg tcgaatccat cgcaatggcc cttacggtgt
    17821 cttcaccgag gtgttgctgg acttccagga tgaggttctt cccgttcttt ttccggacca
    17881 ccagggcgct gagaattgca ggcagggtgg aatccggtcc ttcgaaactg atatccacca
    17941 ccggaccgat tacctgtttg actttaccga tatttgccat atattttatt ctttgaaaat
    18001 gagcttattg taactatttt attgaaaagc gacgcaaaga taaggccaga attcgggatt
    18061 tcaaaaccgg attcagcatt cagcattcag ttatcagcta tcagctatca gctttcagct
    18121 ttcagctgtg agccaactta ccaacttacc aacttactaa catgccaact tgccaacttg
    18181 ctgactacta ccccgctcta agataatctt gacatgttca cggatgactt ctgccagttt
    18241 ctcagtggga aggtcgtttt cgtccccgct aaaggggtct tcaatctctt ccgcgatgag
    18301 ctccaggctg gccagtacat aaaagataaa aaccaccacc ggcacaccta tataacccag
    18361 gctctttcca aatccaaaag ggagggtcat cacataaaaa aagacgaatt ttttaataaa
    18421 tccgctgtag gaaaaaggaa tcggggtgtt tttgatcctt tcgcaggccc cgcagatttc
    18481 cataaaattg gacaattcgt tgttgagggc caggaattgt tccggagaga tcgtatgttc
    18541 cttgcgcatc cggttgacga tgcgggcgat gagctccgtc agttgtgcgg gtacatgcct
    18601 gtcctggtcg tgcagcttca tgacagggtc atcttcatcc agttcaaaca gctccatccg
    18661 gatggtttct ttccggagat gcttatgcaa ggcgagtgca aaggctccaa tcgccttgct
    18721 aaacctgagc ctgagctttt ggtcctcctc cgggatgtaa gtactcagct tgattgccag
    18781 gttgcggctg ttgttgacga gggtccccca ccaccttctt gcttcccacc agcggtcata
    18841 agcggtgttg gtcctgaata cgagcataat ggagatggcg aatcccagaa ggctgtgcat
    18901 ctggatgatg ttctggacat ggaggtcatt gacccatgaa agccggattt cgagatagat
    18961 gatcaaagtg gaataaacgg ccatgcctgc gagcaagggc aggagcttcc ggaaggtatc
    19021 cgccttgtga aatctgaaaa tgaaggtaaa ccagtccttc gggttgtatt gaatcataag
    19081 gtaaggtcgc cggtgccggg attttgttgg ggcaccgctt cgtgattttt gccgatccag
    19141 aaattgttgg ctccttcccc ggagcttata cttacctgct gttggttgag caattccaga
    19201 agggccagaa aggtaatgat cgcgtgaatc cggttttcgc aagggacaaa aatatcttca
    19261 aaggatacct tttctctttc agcgaccagc tccaggatgt agtcctgctg gtcaaggacc
    19321 gtatagatga aattgtaaac cttgtgcaca tgccggcggt catcaaactg gaccagcatg
    19381 cgttcgaagg ttttcatgag tttgaaaagg gttacggatt cccattccgc ctcgatgaaa
    19441 gctttttccg caaacttttt caactcctgg ttgacatttc cccggctggc ctgttgcagg
    19501 cgtttgagtt cgagggcttc aaattcttcg agcacactct tgtagcgctt gtattccagc
    19561 aggcgttgca ccagctcctg ccggggatcg atttcgtttc cttcttcatc gatttccttc
    19621 ctgggcagca gcatttttgc cttgatgcgc atcagcgtgg cggccaccag gatgaattca
    19681 ctggccaggt cgatgttgag ttgttcaagc tggtggatat aatccaggaa gtcggcggtg
    19741 atcttggcta tggggatatc gtatatatcc agttcatccc tttcaataaa aaataaaaga
    19801 agatcaaagg ggccttcaaa ttgtttcagt ttaatggtaa aagacataga ggagaatgct
    19861 ttttatacgg ggatgccttc cggggcaaaa atacccagaa taaggagaaa aataattttt
    19921 cctaaaaata aaagagggga cccttgcgga tccccttccc ggcattcgtc ttgattttat
    19981 acctaactaa ttgccggtaa aatagctcat gatcaggatg acaatggcca ggatagcaag
    20041 ggaaaaaaag acatcccact tgttaaaact gtttcgcgtg gccagttttt gctcgggtgt
    20101 cagcgtactg aatgtcaaac ctttaagtga ttcttcggcc ggcttttcag taagcaggct
    20161 cactccaacc gctaccagga cacagcataa aaagagccag atgctcaaat gcataaaatt
    20221 gtagctagca aaagaatgca taaatccggt caaagaatct ttgtttattt cggcgataat
    20281 acggaccgct gcaaaaatca caccaaacca taaggcagcc atagcccctt tcgagttgat
    20341 ccgtttaaaa aatatgccca ggaggaagac cgcggtgatg ggcggggcga tataactctg
    20401 cacgctttgc aaatactggt aaaggacacc gccgccgatt ttggtcagaa tggggaccca
    20461 caacaaaccg gcgaccacca ccacgccggt ggcgatgcgg cctacccgga ccaggttttt
    20521 ctcgctggtc tccggcctca gctttttata tacatcgacc gtgaagagcg tgctgcaact
    20581 gttgaatatg gaggccagcg aactcatcag ggctgccagc aatcctgcgg caacgagacc
    20641 cctgaaaccg gccggcagga gattttgcat gatcacggta aacgcttcat tcgggttgtt
    20701 cagggtaatt tttccctgtt gcgcaagcgc ataacaaatg atgccgggca tcaggaaaat
    20761 gaaaaccggc gtgattttca aaaaggcccc gaagatagcc ccccgtcttc cttccgtgag
    20821 gtttttggcg gaaagggttc gttgcacaat atattggtct gtacaccaat accaggtgcc
    20881 cacgatggcg cctccgaaaa ccagtccggt ccagggaaaa ttcggatcgg aagaaggccg
    20941 ccacatatta aaatgggtgg cgccgaccag ctcggtgagg gtgccccagc ccccgacctt
    21001 ttgcaatccc aggatcgtca caaaagccgc gcccaggatg aggatgatgg tttgaaaggt
    21061 ttccgtaagc accacggccc tcatgccccc caaaaccgta taaattcccg tcagcaccag
    21121 gaccccgatc gccccggtcc agaaatcgat gccgagcagg gaggaagtca ctatccctcc
    21181 ggcgtaaagg ttgacggcca ccttggtcat gacataactg acgagtgaaa aaatcgataa
    21241 aaaccaacgg gctttcggac tgaaccgttt ttcgaggaat tccggcatgg taaacacggc
    21301 gctccgcatg taaaaaggaa ggaataccca tccaagcaac agcacgatcc aggcgtgcaa
    21361 ttcatagtgc gccatgggca tgccgctgtt tgctcctgtt ccggccaggc ccaccacatg
    21421 ctcggccccg atattggaag caaaaatgga agccccgatc ataaaccagc cgacattgcg
    21481 cccggcaagg aaataatcct ccgtatcttt ttgagaaaat cgcatcgccc aaatggtgat
    21541 gcccaccagg acgaaaaaat acccaacgat gacaatccag tctaaggtta ccaaactgct
    21601 attcatattc attttattaa tgggtgcgta atatcgaaaa aattctctga aattctgctc
    21661 tccattccgg gcatccggtc atataaaaat tcaaaaaaca gggcatttgt catttcaatg
    21721 caaaaagccc gggagtgaaa aattaggaag ccccttaccg ggcttcgccc gattcatcga
    21781 tatttgcccc tatgtctttt acctccggcc agatcgaagt attcaacaag gaagttttat
    21841 tgaagtatca gttgttcaac agccttttta cctcattgcc ctttcagaaa atcgaaaaaa
    21901 cgggtatcct cttgtccctg ctggtcactt attgcgagga aggatatgca aaaggcaaca
    21961 gccctaaaga aatcatagac cattttttca ggactcaaac cgattatatc ggggaaaaag
    22021 acaggctgga cctgctcttc cggtttatcc agtatgggga aaggcaggtg gtattgttcg
    22081 atgccctgga ggacgcggcc tttgccctta cccatgacct cgagggcatc ggtactttaa
    22141 aacaaattga aacggaaacc agggtcaagg cgctgaccgg cgagttggcc gaccggctca
    22201 gggaattcgc cgttcgtttg gtgcttaccg cccaccctac tcagttttat cccgattcgg
    22261 tcctgggcat tattcacgaa ctggctcaat gtatcgccac caaccaaatc accgaaatca
    22321 acctcttgct tcagcaattg ggtaaaaccc cctttttcaa acatacgcgg ccatctcccg
    22381 tggatgaagc caacagcctg atctggtatc ttaaaaaaat attttatccg gcgatcggaa
    22441 ggatcaatca gcgcgtcatc ggcatgatcc cggagaccgc ccgggaacag gatcccctca
    22501 tccacctggg tttctggccc ggcggcgacc gcgatggaaa tccatttgtc acctggcaaa
    22561 ccacggtgga agtcgcaggc aacctgaggt caaacctcct gcaatgctac ctggacgata
    22621 tccgggacct taaaaaaagg ctgaccttta aaaatgtgga tgtactcatc gcgcggttgg
    22681 aaaaaatgat ccttcaactg gccgggacca atggcaatgt ggtctccaaa gagaaaatcc
    22741 tcgacctcct gttccaggtc cggaatatca ttacgaatga gcacgactcc cttttcctgg
    22801 aaaacgtgga tgcttttatc gcaaaagtcc agtcctttgg tttttatttt gcatccctcg
    22861 atatccgcca ggattcctcg gtccatcagt cggtggtcca ggcaatcctt ccgttcacgg
    22921 atcattccaa tgtggactac cctgcgctgg acgaagccca aaaaaaccgg ttcctgacaa
    22981 cccaggcgga tctccatccc gatttccatc ttgaacccgg ctccctggaa gaagatacta
    23041 tccttaccat ccgggccatg aaatccattc aggagcagaa tggcgaactg gcctgccatc
    23101 gttatatcct tagtcaatgc gaatcggtaa gccatatcct ggaagccttc ttcctgatcc
    23161 ggaccacctg ggcacagccc cagatcagtg tggacatcgt accccttttt gaaaaaatcg
    23221 atgatcttaa actggccagc cagatcatga gtcagttata ccgcgacccg gtgtaccgag
    23281 aacatttaaa aagccggtcc gattttcaaa ccatcatgct gggattctcg gatggcacca
    23341 aggatggagg atatcttatg gccaactgga gcatttacca ggcaaaagaa aacctcacgg
    23401 cagtgtccaa ggagtttggc atcaaggtga tattttttga cggaagaggc gggcccccat
    23461 cgcgcggagg aggaaaaaca cataaattct acgcctccct ggggcaccgg atttccaata
    23521 cggagatcca gttgaccatc cagggccaga cggtcagcag tagttttgga accatcgcct
    23581 ccgcgcaata caatatcgag caattattga acgccgggat ctacaatgac gtgttggccg
    23641 atcataccaa taccctaaat ccggatcacc gacaactcat tgacgaaatg gccactacag
    23701 gcatggaagc cttccgcagc ctcaaatcgc atcccaggtt catgtcctac ctgagcaccg
    23761 taagccccct gaactactac agtgagacaa acattgccag ccggccgtcc aaaagaggaa
    23821 aatccaaaga cctccgcctc gaggacctga gggccatccc tttcgtaggg tcctggagcc
    23881 agaacaaaca aaatgtcccg ggcttcttcg gagtgggctc cgcgctcgaa aagatgtatt
    23941 tggcaggtaa atgggaccag gtcagggact tgtaccaatc ttccctcttt ttcaagaccc
    24001 tgatcgaaaa caatgaaatg gcgatgaaaa agagtttttt ccccctcacc gcctacctgg
    24061 aaacggatgc tgaatttgcc ggccttttca ggatcattta cgatgaattc aaaaggaccc
    24121 aaaaatatgt cctcctcctc accggccaca aattcctgat ggaggattac ccggcagatt
    24181 ccatttcggt ggatatgcgg gaaagaatcg tgcgcccctt actgaccatc cagcaatttg
    24241 ccctgatgca attgcgcaaa ccggaatacc aggaccatga actcagaccg gtttatgaaa
    24301 aactggtgat caggagttca ttcgggatca tcaatgcagc aaggaattcg gtttagtttg
    24361 caagttagta agttagaagg ttcgcaagtt ggaaagtttg gataagttct cttctcggta
    24421 aagtttagct ttgggactgc gctttgaggc aaatttttat tgccctcttt aaaagcgctt
    24481 tgaatcagcg tttcttgtcc tatcttcgtt gaatatttac atcatctatt ctgttgaatt
    24541 ttaactattt ggcttcatct atgcatttcc ggaaaccagg tattaccctc cttttgctct
    24601 tttttggatt tctggccaca caggcccaga cctcccttga aggaaaggta gaggacgaaa
    24661 ataaagaagg cctgatcggc gccacggtgg tattatttaa aagcggggtg cagaaagggg
    24721 gtacgacgac cgacctggac gggaattata cttttacaaa tctcgatccg ggcacctatg
    24781 atgtggaatt cagttatgtg ggctatcaaa cccagcgcat tcagaatgta gtggtttttg
    24841 ccggcaaggc aaaccggctt gacgtggaaa tggcaaccgg gatcctgttg aatgaagtcg
    24901 tggtggtaga ctataaagtg cccttaatcc agcaggacaa taccacttcc gggggcacgg
    24961 tcacctccga acaaataaga aacctaccca cgaaaaacat caatgaactg gcagccacca
    25021 ccgcagggat cgcccagcag gacggggggg cgctgaacat caggggatcc aggtccaatg
    25081 ccactaacta ttatattgat ggcatccggg tcaacaccag cctcattccc cagacggaga
    25141 tcgaccagat gcaggtgatc accggcggcg tggaagcccg ctatggggat gtgaccggcg
    25201 gggtgatttc cattacgacc aaaggaccct ccgacaaatt taccggcggt ttagaactgg
    25261 aaagctcaca atttctggat tcttatggat acaacctgct caatttcaac ctgagcggcc
    25321 cgatattgag aaaccagaaa ggcgtttccg tcttaggcta ccggctcagc ggccaatacc
    25381 gcaggcagca ggatgataac cccggcatta caggaaccta ttacactcat tcagggaagc
    25441 tgaaagaatt agaagccggt ccgatcaccc gggtcaacgg agtgccgctt tcctccgcgg
    25501 aattcctggg ttccggggac gtggagttgc gaaaagccag gatgaatgaa caggcgtccc
    25561 ggattgacct caccggtaaa atagatgcaa ggctcaccaa aaaaattgac atcggattca
    25621 ccggagccta taaccggaac aacaaccgtt tttctgccgg atccacctgg gacctgctca
    25681 attatgaaaa caatcccctg gctacctccc aaaactaccg gggcgccttc cggtggaggc
    25741 accgcctcgg tcccgaccct ttggaaaacc cacagggcgg cccgcggaaa aaaggggata
    25801 accggatttt tcgaaatgcg gtgtataccc tgcaagccgc ttacgaaaaa gggaataatc
    25861 aaaatgagga ttaccggcac agggaccgtc tctttaacta cggccatatc ggcaattttg
    25921 atatcagctg gatccccgta gagggtgagt ctacctactc ccgcggcccg atcccgggcg
    25981 tagcccacgc aggttacctg cagcaactca acggttatac gccgggcact ttaaacgagg
    26041 tgctggccaa ttacaataaa aacctggacg ccaacaacct tttggattat tataccttca
    26101 acggttttat ttcaaacaat ttcaactcgg cctggcgcct tcataccaat gtaggcgccg
    26161 tctacaacaa cttcggcaaa ggagaaagcg atatcctcac cctgaatggc gaagccagct
    26221 tcgatctgtt tcccggaggg tccaacaagg gaaggcataa catccaggcc ggcttcatct
    26281 tcgaacagcg caccgacagg gcctggggca tcagtcccag cggactctgg accattgccc
    26341 ggctccaggc caacaaccat atcaaaggtg tggacacttc aaaagtcatc ggttctttcg
    26401 atgggctgca aactccctat acgtatgaac aatttcaaac cctgatcgag gaagacaatg
    26461 acctcctgtt ttataaaagg atcaggcaac ttaccggaca aaccctgaat gaatacgtga
    26521 atgtagacgg catcaatccg gccgacctgc ggctggatat gtttgctgcc agtgaactta
    26581 cggaccaggg cgtactggat tattacggct atgattacct ggggaataaa ttgagcggca
    26641 aagctcgctt tgaagatttt ttcacggccg tcgacgcgga gggacgccgg acctttgtag
    26701 tcgctccaaa ccggccgacc tatgccgctg cctatatcca ggataaattc agttaccggg
    26761 acctgatcct gaggctgggc ctgcgtgtcg accgttatga cgccaatacc aaagtgctcc
    26821 gggacccttt ttccctgtat gagatcaccc aggccaaaga gttttatgcc tctctcggtg
    26881 aagaaaagcc ggccggtgtt ggagacgact acctggtcta tatcaccaac aaaaactcaa
    26941 acaacgtaaa agccttccga aaaggggaac aatggtattt ccccaacggc acgccggcca
    27001 acgacgggtc ggtattgttc ggcggggaag tggtgacccc aaaactgaaa gaccccaatt
    27061 ccaatatccg cctcaaatcc ttcaatccgg accagtcctt tacggactac gttccccaga
    27121 tcaattttat gccgcgcctg gccttctcct tcccgatttc ggacgatgcc aacttttttg
    27181 cacattatga tatcctggtg cagaggcctc cttccaatac catcgccact gccctggact
    27241 actattattt tgaagacccg gccaggaccc cttccaacaa ccccaacctg aaacccgaac
    27301 ggaccattga ttatgaggtc gggtttcagc aaaaactgac gaattcttcg gcactgaaaa
    27361 tcgcagccta ttacaaagag atgcgcgata tgatccaaag ccgcacctat ctgtatgtgc
    27421 cttcgcccgt aaataattat accggttatg gtaacctcga tttcgggacc gtgaaaggat
    27481 tcagttttca gtatgatctc cggaggaccg gcaatattga attgctggcc aattacaccc
    27541 tccagtttgc ggacggaacc ggctccaacg ccaataccca gcgcagcctg aatcaacggg
    27601 gaaacatcag gagtcttttt ccgctgaatt ttgatgagag gcaccggctg gtcaccgtga
    27661 tcgattaccg ttatgcttcc ggaagggcgt acaacgggcc aaggatcaac gggcttgaac
    27721 tctttgccaa tgccggtatc aacttacagg ccaatgcggt cagcggcagg ccctatacca
    27781 aattgcaaag ggcgcagccc ctgggcggga cgggttttgc aggcgccctc aacggttccc
    27841 ggcttccctg gtccttcacg ctggatctgc gtgcagataa aagtttcagg gtgggcagtc
    27901 cggacagccg ttttcccatg tttctgaatg tttacctgcg ggtgcagaat gtattgaacc
    27961 gcaggaacgt ctatggggtc tatcccgtca ccggatcgcc ggaggatgac ggctacctga
    28021 cctccacgga tggcaagtcg gcgctcaaca cggtgacttc caccggcagg gatgtcaacg
    28081 cttatttatt gtcctaccag tgggcagaat tgaatcccgg attttattcc cttccgagaa
    28141 gaattttcct gggcgctatt gtcgaattct aaatccatga tgatgtttca aaagaaatat
    28201 atatgctctg tggtcctggt ggtgttcggc ctggttacgg tatgggctta tccaccctac
    28261 ccgccaaaaa aggcaaagct ggatgagctc aaacaaaggc ttaccccccg ggccgattgt
    28321 gccccttcca cctcctccat agacatgcaa atcaataacg taagggccag gcttttgggc
    28381 ggaggggatg tctggtggga cctgcaggat ggtaaataca ttgttccaaa ggtgcaaccc
    28441 ggatcgggaa tccctgaggt atcctccata ttcgccggcg cggtctggct gggcggatac
    28501 gatccggtgg gcaacctcaa acttgccggc cagatttacc gccagggaaa tctcaacgat
    28561 ttttggccgg gccctctcga tccggtaaaa ggcaccatag gcattgacac ctgtgccaaa
    28621 tgggacaaac atttccaggt cacgggtgaa aatatcgccg cccacctgaa agcgtttaac
    28681 gaagcccagg cttcagggac tgtgctggat tgcaaaaaaa taccggaaga cgtcctcggc
    28741 tggcccggca ggggcaatcc ttactttaaa aacatccacg gctttgagct ccccaatacc
    28801 agccaggggc ttgccggctt ttatgaccgc gacggcgacg gattatacga cccctgcaaa
    28861 ggggactatc ccatcatcga gatccgtgga tgcaacgccc cccagtatcc cgatgaaatg
    28921 accttttgga tctataatga cgccgggggg atacacaccc agacccaggg taaacccatc
    28981 cagatggaaa tccaggtcca ggcctttgcc tatgcgacca acgatgagat caacaatatg
    29041 accttccagc ggtataaatt gatcaaccgc gccacccaaa gcatcgacag taccttcttt
    29101 gcgatgtttg tggaccctga cctgggttgt tatacagacg actacatcgg atgcgatgtg
    29161 agcaggtctt tggcctatgt gtacaacgaa gatgccaccg acggagaaac gggatgctcc
    29221 tgcccgggag gcataaacac ctattgcaac gatgtgccca ttctcggcgt ggattatttc
    29281 aggggccccc tggatgaatt cggccaggaa atcggcatgt cctccttcac ttattttaac
    29341 aatgctgcag tcggcaactg gcccaacgcc atgaccgatc cgcaaaacgc gcaggaatac
    29401 tataattaca tgtccggatc ctggaaagac ggcacgccct tttcttacgg aggcacggct
    29461 tatcagatcg gggctgccag aaatgattat gcctttaccg acgccccgga caacgcccgc
    29521 ggctggtcca tgtgtaccgc aaacctggac tttggagacc gccggaccat ccaggcctca
    29581 ggtcctttcc ggctggatcc cggtgcggtc aatgaactca tcattggtgt ggtttgggtg
    29641 cccgatttgg attatccctg cccgaatatc gaccggctga cttcagccga tgacatcgcg
    29701 caggccttgt ttgacaattg ttttgacatc accgacggac cggacgcacc ggacctcgat
    29761 tttatagaac tcgaccagga actgattttg cttttgtcca atgacccggt gaaatcgaat
    29821 aatgcctatg aaggatacca ggaagtggat ctgcaagccc ccgccgggat tgccgacagc
    29881 ctctatcgtt ttgaaggata taaaatttac cagctcaaaa gtgccgatgt cggcatcgcg
    29941 gatattgagg acgtcaaccg ggcaaggctt atcttccagg tggatatcaa aaacggggtg
    30001 cggaatgtat ataactggaa atcggtgcca gaccccaatt ccacccagcc ggtttggatt
    30061 ccggaaatcc gggtggaagg agaagatgcc gggatcaggc acagtttcca gatcagggaa
    30121 gaccagtttg ccaccgagga ccgggccctg atcaaccata aaaagtatta ttatacggcc
    30181 atcgcttatg cctataacaa ttacctgcct tttaatccca gggatgtcgt cggccagcga
    30241 aaatcatacc tggagggcag gaggaatatc cgtacttatg ttcccattcc aaggcccgtg
    30301 gtgaatacca atctcaagtc cgcttatggc aacggcgtgg agatcacccg gctggatggc
    30361 atcggggcag gcggcagttt cctggatatg gccgattcca cccgccaggc tatcctcgag
    30421 aaaaaatttg atggccgcat caaatacaaa ccaggccgcg gtccgatcca ggtgaagatc
    30481 tataatccgc tggacgtgca ggatggtgaa ttcatcctca cctttaccga tgcagacccc
    30541 aacaataacc ggctcgaccc gccggtcaga tggaaactga taaacagcaa ggacccgaat
    30601 acggtcatcc tctcggagac caccctggat aatctcaatg aacaattgat tgccaaattc
    30661 ggcttctccg tcacgctcgg ccaaagcatc gatgccggcg atgatcctct gggtaacaat
    30721 ggagccatcg gagccgagat cagttataaa gacctcaata aaccgcagtg gtttgggacg
    30781 gtgccggacg gagacggggt aatcaattat gtcaagacag gcaaaaatga gatcgatttt
    30841 ctcctggatc agaaatccgc cttgtccact atgggcaatg gcggcggttt tgtccccttt
    30901 tacctcaccg atttccgggt ttcggatcct ctgaatcctt ttatcggtcc atcctggatg
    30961 gatgcctcgg atggtttccg gatcatgcgg gaaaacccgg ggttccggct ccagaatcta
    31021 aataacgtgg atatcgtgtt cacgccggat aaaagcctgt ggagccgctg cgtggtggtg
    31081 gagacggctt cccctttcta ttattcccaa accaccggcc tgggcattcc cacctttatg
    31141 gattccagga atatggattt gcgcagaagc ccttcggttg gcaaagagga taccaacaac
    31201 gatgaccttc ctgatccgga caacgacgga accggaatgg gctggtttcc gggttatgcc
    31261 atcgatgtgg aatccggtaa aaggttgaat atcttttttg gagaaaattc ggtgtatgac
    31321 ggccggttgt ttccccaggg cttcgatgat caaaaaccga tcggagggga tatgatgtgg
    31381 aatccaacca gtcagcaggt gatccagacc ggcggccctc ctaccctttt taattatctc
    31441 ctggggggac accacttcgt ctatgtgacc ttgcaggaat acgatgaatg caaggccttg
    31501 cgcgaacgtt tgaattccgg gaacagccct ttgcggaaaa tacccggact tcgcgacgtc
    31561 acctggacct tgtggcccat tttgcccaca ggacaaaagc tgctgtccta cagggacggt
    31621 ctcataccca atgaaacgat cattaagatg cgggtggaaa acccattcca ggtcgcggtt
    31681 ggcaccaaca gcgccaaagg tcatccctcc tacctgttta ccatttcggg gcaggccgca
    31741 cagcccttgc agggcgttga aataccggct gcccttaaaa aaataaacct ggtgcccaat
    31801 ccctattacg cgtattccag ttacgaatcc acgcagtttg ccaatattgt aaagatcacc
    31861 aacctgcctg ccaaatgtgt agtcaccatc tactccctcg atggcaggtt catccggcag
    31921 tacaagcggg atgaaaagga tcttcctatc cgggaccgga ccaatcccgc ggtgctgaat
    31981 acccggtata tccccgacct ggaatgggat atgaaaaatt caaaagggat accggtcgcc
    32041 agcggcgttt acctggtcca catccaggcc gaagggatcg gggaaacaac gattaaatgg
    32101 tttaacgtca accgcaaatt cgatcccacc ggtttataaa gcatgaagtc aatgaagcgc
    32161 agatactggc cgataatttt attgatcccg gtgatctttt tcgccgatgg attgtatgcg
    32221 ggcaacccgg acaggcaggg ggaagccggg gcatatgaac tgaccatgaa tccatgggcg
    32281 cgggccgcgg gcctccatac catgacgacg tctatgatct ccggtgtgga agcgctttgg
    32341 ctcaatccgg caggcctggc ccggatcaac cggactgaat tgcttattgg acatactcaa
    32401 tatctgaggg gatcgggcat cagtatgaac gccctgggtt ttgcacagaa aatgggtaaa
    32461 aacggggtcc tcggatttaa ccttgtttcc ctggatttcg gagatatcct ggtgaccacc
    32521 acccaattgc cgggcggaac cggggcgacc tattcaccca gtttttttaa tctcggggtc
    32581 agttatgcat atacctttga aaacaaggtc tccgtgggta ttctgcttcg tggcatttct
    32641 gaatcaacct ccgacctgag cgcctttgga ttcgccattg acgccggagt ccagtacgtt
    32701 aacggcccgg aggataattt taaatttggg atctcgctgc ggaatgtggg ctctcccatg
    32761 cgtttcggcg gagagggcct gtccttccaa ggggacaacc cggaagggga aaccccttat
    32821 caactgacct ttgaccagcg gtctgcaggt tttgaattgc cctcgctcct taacatcgga
    32881 acttcctatg attttcacct ggacaagagg aaccggctca cggtcctggc caattttaca
    32941 gccaattctt tttcacggga tgagcttgga gccggggtgg aatatgcgtt caacaacatt
    33001 ttctttcttc gcaccggtta caaatatgag ttggaaggcg tggacaataa aaatgtctat
    33061 acgggtttgt cagcaggctt cgggattgag gctcccttca aaaaagggag caatcagaaa
    33121 ctgggtatag attatgggta ccgcgcgagc aatccctgga acgggagcca caatatatcg
    33181 ctcaaaattt ccttgtaata gctgctttaa tccaatttga attcaggatt ataaatcaat
    33241 ccgatccggt ttccccaggg gtcggccacc gtcgaaacca tgatttctcc acctacgttg
    33301 tgaggtattt caactggggt cgcaccaagc tgaatgaacc ggtcaaaggt ctcctggata
    33361 ttttccgtgc cccagtaagc cactgcattg gccttgtttt ggtccccggt atagccatcc
    33421 gggtccaggc ccaattcata acctcctata ttgaatccga cgtaaaaggc ttcgtcaaaa
    33481 tagggtttgg tttcgaaggc cttgctgtac caggtttttg ccttttccag ttcaggcact
    33541 ttatagatca ccgttcggag tccgaggagg gatgaagcca tcatattaat tttatttttt
    33601 gtaaaaatac tctatgaacg atgaattcta agctggcggc acaacgaata atccggcagg
    33661 gcctctccca tcctcaattc ggcgatccgg tttcggtggt gaaacatatg gccgggatcc
    33721 aggcccagga tttcgaaatg gccaagtggg cggtggggat ccgtatgcag aacccggacc
    33781 tggaggccgt caatgctgcc ctggattcgg caaagatcat cagggctcat attttcaggc
    33841 ccacctggca cctggtcccg ggagaggatt attggtggat gaaccggttt tcggcaccat
    33901 ttcttagaaa aggacatacg ggcaggtttc aacaactggg attgaaggaa accctgttgc
    33961 agaaaaccag gaaactgatc cataaatttt tgtttgcccg gccctctctc acccgtcaac
    34021 aaatcctcga agtttttcaa agccataaat tcaaaccgga ccctcagcaa cttaaccata
    34081 tcctttacga cgcagagctg gaaggcctta tttgcagtgg cccccaacaa ggaaagacgc
    34141 attcctatac gctggtggag caaagactgc ctaccccggt gggcaaaacg gcggaagagg
    34201 ctttggcaga actgaccatg cggtatttta aaagccatgg tcccgctacc cttcaggatc
    34261 tcaccaagtg gatgaatatc cctgtaaaca ctgcccggct gggcatagaa atgaaccggt
    34321 cctcgcttga ctttcaaacc ataaacggaa aagtccattg gttcacagac caccctgaac
    34381 ctgcacgcag ccgaccccgc ccctttctcc tcttgccggc ctacgatgaa tacctgattg
    34441 gttatgctga ccgtagcttc gccatccaac cggagttt
//
LOCUS       DAOQXT010000044        33511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1522_length_33511_cov_8.646730, whole genome shotgun sequence.
ACCESSION   DAOQXT010000044 DAOQXT010000000
VERSION     DAOQXT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 33511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33511
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_33511_cov_8.646730"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="PKM27_09445"
     CDS             <1..1711
                     /locus_tag="PKM27_09445"
                     /inference="COORDINATES: protein motif:HMM:NF037041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07526.1"
                     /translation="PLPEAKTFFYQISWNLITILLTLCPIRMEIGKKLQTSHLWDHLF
                     EDSQLKKDLEELVLPDYIITCRWFAGKSSTIKHVRVDHAHRLADKKQAYYILMLEVQF
                     KEAFSHQYLLPLAFIPDEEEAQYTEVPVISRLRADNREGFLIDALFTADFRNLLFDLM
                     VKNKEMPLNQGVLQFESSTSFKPPTGKKVFSEILKTEQSNTAVIYNNRYFFKWFRRLF
                     PESNPDLELSRFLNLNHFPYTAQYMGSISWLRMDKPRISLGLMQQKLDNEGDAWKWML
                     QEVNAFFSGFDQNHQLQLTWEPKETLFDPVKPKNLSPQTRSQLGKKLMKGVVRLGKRT
                     AQMHLALARDNGQTSFTPVAFNPDYSVWLKNKVTYLFDLRIGLLEKNIGKLRGLAREY
                     GQVFLDRRNEVLEEILSFDETTLCSMRTRVHGDYHLGQVLVHEDEFYILDFEGEPEST
                     IRDRKVKQSPLKDVAGMLRSFHYAIYATLFDEEKNWTLPQHDLFDLAERFYQLLCGLY
                     LHGFIRTAVRGGLDIGYRPEIHYLLRYHLLEKAVYELGYELNSRPAWAIIPLRGVMRI
                     MGF"
     gene            1824..3752
                     /locus_tag="PKM27_09450"
     CDS             1824..3752
                     /locus_tag="PKM27_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR07527.1"
                     /translation="MSTLRQLAAIVFVDIEGFTALMQRNEFLAMQWRERLKRKLQEET
                     EKHFGKILDFRGDGALCSFTSTTEAVLAAIAVQIEMQKEPAVPVRIGLHTGDVVVNEN
                     SIYGDGVNIASRIESFAVPGSIFISGKVYDDIKNQKEIQSVSLGKFALKNVLEQMEIY
                     AISNPGIKIPGEINLLGKGEKIQPNQVLVLPFVNMSNDPEQEYFSDGLTEEIISCLSQ
                     IHEVKIISRTTSMKYKGSKESLKNIGRETGAAYVLEGSVRKAANLLRITAQFVDAHRD
                     TYLWTETYRGTLDDIFDIQENVSARIAEALRIRLTKNEKDNLRKRYTDDSEAYQLYLQ
                     GRFFLKKRNAAGMKSAIRHFEKAIEKDPDYALAWAGLADTYSLMGEYTNISRRELLPK
                     QVTALNKALEIDDRLGEVHISKATALMLNDWDWVNAEKEFKLGIELSPKYATGHHWYA
                     EWLLYSGFPDAAFEEINLAYRLEPSSQVIVKDLGMYYYYTRQYEKAVEMAFTALELDP
                     EFVSSYRLLSLAYLALGNTEKALEANRQWGEKSLNWMKTELGLAYILAYTGQEEDARK
                     ILEREGFENELGHNDCRGLALVYTALGDRDRAFHWLEQGINRREESLTSIQTDPKFDP
                     LRKDPRYTEVLKKIGLYR"
     gene            3950..4492
                     /locus_tag="PKM27_09455"
     CDS             3950..4492
                     /locus_tag="PKM27_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07528.1"
                     /translation="MSLRILLFSFLIPLTGVPASAQPVKKDTLNQPTIKGYHIGVVQP
                     LLAIHQNKTSYMTEYDFYSIGFPFGLTLRTSGRFLIDLELVPFIKPYADDAGKPYDVH
                     LLYHPGVLYPLGSGFTLGLRAAFESGQDQFGFTPLINKSFKIGPSGSFFVELVFPGRF
                     GPEKSSGYTQVGGIHIGFGF"
     gene            complement(4530..4979)
                     /locus_tag="PKM27_09460"
     CDS             complement(4530..4979)
                     /locus_tag="PKM27_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNR07529.1"
                     /translation="MFSKSTEYALRAIIYLAQNSDPDHKIGLEELSKAIGSPRSFTAK
                     IMQKLTKDQNLVSSVTGPGGGFYTNQESKTRSVFSVLELMGEETTITQCVLGLDQCSD
                     QNPCPLHSTYKQIKPRLLEMFQGKSIQQLAKELKNEKTVLNRPRKAS"
     gene            5091..7475
                     /locus_tag="PKM27_09465"
     CDS             5091..7475
                     /locus_tag="PKM27_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNR07530.1"
                     /translation="MKTTDHHLNGLTKSISYFMNTQNWWGPLLFIFIISVLGVGMIGF
                     QTYHDAPPLSGFKNQEGEIILDKAEITKGQLVFHKYNLMEYGSFFGDGAQRGPDFTAE
                     ALNQVAKNMKLYYSGVYQDEKGVTPGEEAIINIEARIKKELKKNTYRKEEDFIYVSPA
                     LAFALGKVREYYLDLFFENKEGRAFLPKNYIENKEEVAALGSFFFWGAWVCVTERPGK
                     TFSYTHNWPFDPEAGNTPTSPVILWSVLGLLGFVLACGIVLYFIGQYNQLPNKFFKPA
                     TQDLFTMERVRNYTPSRTQRATFKFFLVAALLFFLQVSSGIITINDFVNFLGYIGIHI
                     TQGLPVTIPRSWHLMLALYWISACWIASSIFILPILAKTEVRGQLRLINVLFVLLFIL
                     VGGSLAGMVLGPLNMLGDYWHLLGSQGWEFVDFGKIYQVLLMVIFALWAVIIYRGIKP
                     AFVKGQHWNLPNWILYSVIGIPLLFLSGFVAKPETNFVIADFWRWMVVHMWVEAFFEV
                     FITVIISYLMVLMGLVSKQAAIRVVYFATLLFLGTGLLGISHNFYWNAKPVATMALGS
                     VFSTLQFVPLILLTVEAWRFKNTPKIAVGEVEHKNLGDFGFPVVFLFLVAVNFWNFFG
                     AGVLGIIVNLPIMNYFEHGTYLTINHAHAALMGVYGNISLAALLFASSLLIKAEQWNK
                     KLLLVSFWSINIGLMLMVVLDLFPAGAIQFKAVVEKGLWYGRSHHFIENGTFISLTWL
                     RGIGASLFFFGGVIPFAWFMVTRSRHLKAADLRQEFEMKSSPEPSPEPELEEVY"
     gene            7607..8323
                     /locus_tag="PKM27_09470"
     CDS             7607..8323
                     /locus_tag="PKM27_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNR07531.1"
                     /translation="MKFDYGVPEFYVSDPNINHFNSPPDHPGPRLNKSSRRTFHFPKN
                     VHKNAPADLPHSVDIPSRHSLDPYLWDPGFLCDYVVRIHHDYIQKALPVLLAMGKKVT
                     VLHGNTQVETLAILEQMEMLFSDLKLHLFKEEQLIFPYIRSLVCLEKQGKTIDITRDG
                     PLYKPMAGLEEDHGLFEAGMDAIRDLSRDFTVPVQNSPVFSSWYGALQAFREDLIQHT
                     RLESRVLFPKAIALQRKLTA"
     gene            8607..10616
                     /locus_tag="PKM27_09475"
     CDS             8607..10616
                     /locus_tag="PKM27_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07532.1"
                     /translation="MLIFKLEDDFVQGYKHLDPGFGFNGLGLLTYFRTYSRVKPDGNN
                     EQWYETVRRVVEGAYSLQKQHILSNELGWNPKKAQRSAQEMYDRIFNMKFLPPGRMLW
                     ALGTPIIHEKKIGQALFNCAFVSTDEIGKSTEDAVKPFLFMMDMSMVGVGVGFDVAGG
                     GMITVNKPESEDVDEFMIPDSREGWVESLSRLLTSYFSKHFDDGGAHPVRFNYEEIRA
                     AGLPIKGFGGVSSGPEPLMRMHEQIRSILDQTDGQLLSVTTITDIMNLIGQCVVAGNV
                     RRTAQIAIGDIENKEYRKLKDYRWNPESGQYEGSMAHRASWGWASNNSVLVDRNSDFK
                     PIAAQTAINGEPGYVFMDNIKAYSRLCDPADYKDEKAKGTNPCGEQSLESYELCCLVE
                     TFPSRADSYEDYMRTLKFAYLLGKTATLVSTTWHETNRVQKRNRRIGTSVSGITNFLD
                     KHSLETLRVWLNSGYQEIQRWDSIYSSWLCVPRSIKTTSVKPSGTVSLLAGVNPGCHF
                     PEFEYYIRRVRVAKNSTLVPAIREAHLPIEDDVVDPSSYVVEFPIHNPGKSLKKVSIW
                     EQVALAVFLQKYWSDNQVSCTVTFRPEEANMIHVVLDYFKYELKSISFLPQLEMGAYA
                     QMPYETITREKYLDMTAGILPMQFVEMKEDAEMEKYCDGAVCLRI"
     gene            10814..16021
                     /locus_tag="PKM27_09480"
     CDS             10814..16021
                     /locus_tag="PKM27_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07533.1"
                     /translation="MFFHLLPFILALLTSTGPNEEARPQEACLPNGIILNNQSQIDAF
                     PGLYPGCDSIEGNVFINGAVSNLDSLKGLRAIGGSLFIYATSLKNFSGLEGLRSVGGE
                     LSVFSNFHLISFSGLDSLKELGSRLSIYQNDSLSDILGLSGLLDLKNNPLRISFCPRL
                     STCLIPAVCGHLASGGSAVIHDNAPGCNSRFQIAKACDPGALCPSVNIEIHGQEDLND
                     FISLYPECEDIAGSLNLISVEDTFYSLLPLAGIRTIGNVLNIVSNPFLSDLKGLDSLL
                     SVANGLTIHSNPRLKNLDGLGSLQWVGNTFQLVLNDSLSSLEGLGQIDPDSLHTLYFQ
                     FCPLLSACDHASICQFLSGGGLAYLDGNAQDCNTSHRIQKHCYPDSLCPPGNLYFRTQ
                     EEVNDFHILYPNCQDLAGNLSIDGRNINRLDSLFGLKSVAGQFYLSNNDSLEHLNGLS
                     GLQSIGQNFYILLNPALLSLAGLESLRQIGVGLVMDNNPLLQDLSALADSLLGTNPDF
                     YLRIFYNPALALCHVPAICNYVNQGGEADIEGNAPGCTSLNEIILNCNPSQDCPPGNI
                     EIRSQADLDFFTLNYPDCDHIAGSLTINSEQGPVTSLASLSHLKSIGGSFYIENNPAL
                     ASLSGLDSLEFVGEALSIGSNAGLQSLEGLESLRIIGDYLAIYLNDALLRLEGLENLN
                     PDSLDYLYVYECSQLSECNLPGFCSFLIEGGIAVIRDNAPGCDNPFQVIKSCQPGEIC
                     PPGHQAIYNQQQLDEWVAAYPDCTDLPGSLQIGPAEEEDPILSLTGLAKLGTIGGTLE
                     INQNRWLKSLQGLDSLQGVGADLRIYSMDSLENLLPLKSLKLIEGQLYIRDNPVLTSL
                     DGLDQFDADKLDALFIWYNEQLSFCSNPAVCAYLSTGKSAVLSENAPGCSSLNEVRPG
                     CGVSCSQDFFEFYSQDEIDAFPANNPGCQNIAGYVFISGSDITNLDSLRQIKSIGQDI
                     TIFSNPVLNDISGLMNLTSVGSYVDISFNPLLTTLDGLSGLRSIGGYLGIYSNNILDN
                     IDALYQVDHGGIQELYLQFNPRLQACNAEVVCAFLSDETKSGLAYINDNKPGCNNPSE
                     VKTNCGLANCPVGNLTLSSQSEVDGFRSAYGDCAALPGDLIINGTDIVNLDSLSNLYQ
                     IGGQLIIENTGLENLHGLDHILYTTISHLSIRNNPSLAVCDVRAICDFLSLSGGTFEI
                     IGNAQGCATESEVRYSCSKTCFWDGVGFTSQAQVDSFRRLYGNCEVIPHAVTISGSGI
                     NRLDSLFNLKKIGRGLTVLSTSVPDLDGLAGLDSVGGSLDIRSNSQLLNISQLQNIRS
                     IGSTLRIQSNAKLTSLAGLGRLSGVPFNLTVQSNSALASLSGLDSIGYVGNTLLVMNN
                     PQLNSFSGLRSLKTVGLDCRLETNNLVMNFQGLEKLESVGNDLRILNNTGLQTTVGLS
                     GLKTIGRHLRIENNGGLDSLSGLSALESIGSTFQLINNASLNHIHDLSSLRHIGQSLI
                     IRNNSLLSSLNGLDQVEADPLDYLSIENNPFLDLCNVRSVCLYLFDPDKTYSIFGNAI
                     SCQTGDQILASCSIALPVSLISFTASLEQGQALLQWNTTGDLRPDLFMIEHSRDGERF
                     SLLDKMPFGHQSHPYTYRHSPPPGMNYYRVKAVAEGRSSVYSSIGSVQIKNENWVLPN
                     PTRGILHIRPPDEQRCRIEVRDLTGRVLMNVAGQGPQIMDLSGQPSGIYLISVLSYSG
                     RRIFKVLKE"
     gene            complement(16129..17346)
                     /locus_tag="PKM27_09485"
     CDS             complement(16129..17346)
                     /locus_tag="PKM27_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR07534.1"
                     /translation="MQRDERAIVFLLALVNFTHILDFMIMMPLGNYLMPYFDISPAQF
                     SLLVGAYTLTAAFSGFAAAFFVDNHGRKKILIYAFAGFLAGTIACGLAPGFNLLMAAR
                     VLAGLFGGLIGAQVLSIISDIFGYERRGLAMGAVMSSFAIASTLGVPFSLYLANAISW
                     HAPFLFVGILGIAIIPLLNKYIPEMEGHVNQENPASKLKILNEVIHNKYQRNALMFSA
                     LIMMGHFMIIPFVNPYLEFNKGFSKALTPTVYLVGGLSAFFSSFLLGRLADHAGKVRV
                     FTWSVAACMVVVFIITNLPDWPFPLVLFFFGLWFMVATGRGVTAQAMLSNVPLPAQRG
                     SFMSFNSSVQQLGTSAASFLSGAIVIKGLNGKLLRYDWVGYVSIAILLASLYMGRKLF
                     GDMDQTKKQIQNP"
     gene            17513..18151
                     /locus_tag="PKM27_09490"
     CDS             17513..18151
                     /locus_tag="PKM27_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07535.1"
                     /translation="MLKKYFSFNHWIVLGIIILVELIVNLLLKATLLTEKVLYNSLVE
                     KMTLEDISSFSSTVRSSAWMLVVFGVAQRIGEILLISVCISIGTLLFRYDIQFRQILK
                     VVILSFLVFTVSRLPLLITLLSGKVEKFEDLSFMPNLSLADWFRPENLPEWGLYPLQL
                     INVYQFLFILLMAAGFNRIDPRGIPKWLGLILLTYGTGLAITVVLTAFATAI"
     gene            18349..18546
                     /locus_tag="PKM27_09495"
     CDS             18349..18546
                     /locus_tag="PKM27_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07536.1"
                     /translation="MKQLSLNMMANLEGGGAPIAENRACLSACALYSFACANGLGFLK
                     RLASRLIDVYCKGCHCPDTTM"
     gene            18622..18810
                     /locus_tag="PKM27_09500"
     CDS             18622..18810
                     /locus_tag="PKM27_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07537.1"
                     /translation="MKQLSLNKMASIEGGAALENRACLSACALYSFACANGLGFLKRL
                     ASRLIDVYCKGCHCSDTV"
     gene            18961..19152
                     /locus_tag="PKM27_09505"
     CDS             18961..19152
                     /locus_tag="PKM27_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07538.1"
                     /translation="MKQLSLNKMASLEGGSSTAENRACLSACTLYSFACANGLGFLKR
                     LASRLIDVYCKGCHCPDAF"
     gene            19301..19492
                     /locus_tag="PKM27_09510"
     CDS             19301..19492
                     /locus_tag="PKM27_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07539.1"
                     /translation="MKQLSLNKMASIEGGIGTENRACLSACALYSFACANGLGFLKRL
                     ASRLIDVYCTGCGCTDSIK"
     gene            19683..19877
                     /locus_tag="PKM27_09515"
     CDS             19683..19877
                     /locus_tag="PKM27_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07540.1"
                     /translation="MKQLSLNKMAILEGGGGSSENRACYSACALYSFACANGLGFLKR
                     LASRLIDVYCTGCGCNFDHT"
     gene            20055..22223
                     /locus_tag="PKM27_09520"
     CDS             20055..22223
                     /locus_tag="PKM27_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNR07541.1"
                     /translation="MSQNQQFHVQQHDQSDCGVACLLSVLRSFGGNLPLSTLREWSGT
                     TITGTTMLGLYQAALKLGMDAQGFEADTEHLKTLEHPAILHVQTREGTLHYMVCYAWY
                     PDISQFEISDPAEYGIKFYSPKDLDALWQSKALLVLKSTPEVKKVSRNNSFIQKMRWL
                     YGFARPDFDLLLVSFFIGVFVSLLGLSVAIFSQKLIDQILPEKNLTRLFVGSGLLLFL
                     LLLRSGIGYIRQLFLLRQTRDFNVRIIRFFYSSLLSLPKPFFDTRKTGELISRMNDTA
                     RIQQTVSNVFTSFTIEAVVILITTIALMFYHLQLGLLSLLWLPVFGLIVYRYNKPLIE
                     GQQKVMSTYALNESNYIDTIQGVGVIKLANAEARFTEQTLGVYHVFQEKRFELGQLVN
                     RFGTYSQIAGSVFIVGLIVFSSLLVLRNSISIGTVMAVIQLIGMAMASASSIALINIV
                     LQEAKVALDRMQEFTALPPEYDPLEEKEKRTLVSFDSLEITDLDFRFTGRPLLLDHLS
                     FNIKRGEIIAILGESGSGKSTLLQIIQRFYLPESGTLLVNGVPLHEFSIPEWRKIIGV
                     VPQDVKLFNASLLENILLRPVEEADMAPLGSFFEEHGFNKPFSKLAGGYHTLLGEEGI
                     NISGGQQQLVALARALYHRPQLLLLDEATSAMDRFTEQTILKLLLAIRPHTSTIIITH
                     RPKTASIADRIYILENGQFSVSGSPDELLKSKNFYSEALI"
     gene            complement(22304..24232)
                     /locus_tag="PKM27_09525"
     CDS             complement(22304..24232)
                     /locus_tag="PKM27_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07542.1"
                     /translation="MERNLKMAGANRPELEKAILHFSTNPADSLKKQAILFLISNMEE
                     LYQYEGEWLQRFDTLFYPQIGDYSNAQTDSVRKSLERLIGPLQMKKLSRKGDLKHLAS
                     EFLIKNTEAAFAAWQNAPWKNQVSFDAFCNYILPYKCYSEYAEDWRSGLRARYRHILD
                     DSTNGQSMVKVTCALVNEQLTWFEFSRTLGEYPSALSIGQILLGKKGDCIEMASLGAY
                     SARALGIPVAIDYVPQYGNVNDTHKWDALILGDSESLPFEGGETEPGEMSYLREGDHK
                     FAKVYRRRLGWVESSFAAKARTSGIRNLPEFVSDPRVQDVTQSYTRVGDIRLNIKGRD
                     GTPVYLCVFKLREWVAIAGAMIRNDEVEFSNMGRGLVYMPMLYKNHRYLAAGSPVLLP
                     ASGGQQLITIDPEKTKDLILYRKFPFKRRWKQFLESTMEGARFEAGRDSIITNPVLLY
                     QVPKAKTVYLPRVQNDLSLKDRAGYESVWNQVDLQLSDSFRYARIIFDKNLTFRLGEM
                     EWYTPGQSGPLSGKPIGNIPRPELAFDGFPGRTIKMKTDSTKAHWAGLDFGRPIVLEK
                     IRYLPATDANAIREGASYELFYWKDKWISAGTQTAKDFALEFKQIPGNGLYWLNCRGC
                     NSKEERIFTYEGGQQVWW"
     gene            complement(24452..26539)
                     /locus_tag="PKM27_09530"
     CDS             complement(24452..26539)
                     /locus_tag="PKM27_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNR07543.1"
                     /translation="MYYHTMKIIHIRFYGGQRILSRNAGPGQPGLFSLFILLMLGACT
                     RMEQGNQNALERNLVFSGKNRPEMEKVLARYARHPEDSLKFKAAVFLISNMEGHKHPS
                     GKWIEQFDPIFNHTAGVENEEIQRLKDSIEEKIGRSNEGEVEMLYDLKHLTADYLIRN
                     IDEAFEAWQNAPWRAEVGFEAFCDYILPYKSFSEPPENWRSTLRKKYQYLLDDPEIPD
                     AMEDICCALVKEEGGWFGYSEVFTDYPAPLSIFQILNGKKGNCVEMSNLAAYSARALG
                     IPVAIDYTPQWANFAGAHMWNALILPEGAFWPFIGVEGFPGDYRSISEGEGKIAKAYR
                     HKLAYDSASFAARSRMAGIRDIPERLSNPRISDVTRFYTQVRDIRFTIEAKENTPVYL
                     CVFEPGSWKAVSGALVHNHSLLFTDMGLEALYMPMYYENRTYKAAGPPVLHRINAPVQ
                     ELKAHSHQGQNLVLYRKNPLKRARIYNYMQHPLYHSRLEGSHTPEFNDPVLLHEIPPI
                     AGRYRPRYQRDLTLKDQSSYDSLWREDFIPDPDSFRFIRFLFPKDAPPRLGELEVYES
                     ATGNPLKGIPMGNLPHPEFAFDGVPGRSIKPDSLQMDSAWVGLDLGRRIPVGKLRYLP
                     ADDPNSIEPGKKYELYYWDGRWKSAGTQIATGVSLEYAGIPAGTIYWLRCRDCGNREE
                     RPFTYENGKQMWW"
     gene            complement(26632..28632)
                     /locus_tag="PKM27_09535"
     CDS             complement(26632..28632)
                     /locus_tag="PKM27_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNR07544.1"
                     /translation="MHVNGWFKFQPGLILFVFFFIGSCLPDKKKIGDALETNLALSGK
                     NRPELEKVLNHYRRDPGDSLKWKAAVFLLSNLEDKVHYDSEWLRTYDSMFSEKAAWLD
                     NEQIQSFSDSIKSVIGNPEKKDLREKLDLQYLTSDYLIRNIDQAYSSWQNAPWKSEVS
                     FNAFCNYILPYFNYNEFPQDDKSVLYDKYQYLLKDSLIPHTMEDLVCAQIDDQITWMK
                     YSEELDYPGALNMQQILKIKKGDCVEMANLGAQAARAFGIPATIDYVDGHHWNALILS
                     DENFEAYSGAGDRPGDLSLLREADSKFTKVYRKQLARVPESFGAKAGEAGIKNIPEFF
                     RSPRITDVTSQYTLVTDLEVRANGKDGDPVYLCVYRKHGFEPVAGEFISNHRAIFRQS
                     GQREVYIPMVFKKGKYEAAGPAVVVPVKGSPRELVAREDQLQTMVLTRRYSFKRWMEW
                     ILEAPMISARFEGGRDSAFTNPVLLHEIKRIRKIYRSSVYSNLDLKDRFEYDSIWKQV
                     VPARTGPVQFIRLVFQKGKPLKLGELEFYSQGPQALKGKPMGNIPDASLAFDGVPGRS
                     IKQEDTSKSYWVGLDLGEAKQIDKIRYIAAGDSHEVEAGRIYELFYWKDKWKSAGKQK
                     ASSPSLVFDQVLGEGLYLLRCYNCENTEERPFTYENGKQVWW"
     gene            complement(28651..30693)
                     /locus_tag="PKM27_09540"
     CDS             complement(28651..30693)
                     /locus_tag="PKM27_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07545.1"
                     /translation="MKGKIWNIAKAMGTGLLACMLAAGMVSCNEQPTPAGDVLELSMV
                     YAGDNRPELEKVIDHFSRIAADSLKKKSAIFLITNMKHKVHYGGRWIEKQDSIFSRAG
                     EMDREGILQWLDSFRRHTPPSVKADLRMKHDLQHLSADYLIQNIEQAHEAWENAPWKD
                     LVSYDAFCHFILPYNSLTEYPESWRAWLKEKYGDFVLRREDLKTMEDYCCAFVEEEKS
                     WFRYSEVFFDYPSTLRISDIFKAKQGACVEMANLAAYSARAWGIPVALDFTPQWANHT
                     TGHTWNALITSDSSFIPFLGAEGAPGDFNGVSTGETKTARIYRLMQAEVETSFARAAK
                     IRGIEALPPYLKNPRILDVTGSYVPTASCSIAVRGKEGTPVYLCIFKQGQWNAVAGGF
                     IEDDKVLFVNMGRDIVYMPMFYQNNDYRQAAPPLHVDETGVFRHLVADRKEPTILRLE
                     RKYPLKKFRTKWTYAQYLIHARFEGSDEPEFKNPYLIYKAPEPYTWFATNTIGGKPAR
                     DRLEYHMLWEGTRLNPIKKYRYVRMVANEGNAFKLGELEFYSPDDSLPLKGKSIGTVP
                     HPERAFDGLPGYSIIKEDAVDTERWVGLDLGESKPIAQIRYLPANDQNQVQPGKQYQL
                     YYWKEKWISLGIQKAETHCIHFKAPPGAVLFWLHCADCSSTEERPFTYEKNRQVWW"
     gene            30750..33209
                     /locus_tag="PKM27_09545"
     CDS             30750..33209
                     /locus_tag="PKM27_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR07546.1"
                     /translation="MDFAELKHHRDDLALTLTLFVAFIAGSWSVSISLPFVLAGLLFV
                     LLRLRTQDKPVPFHAVDLAVLAVGLYELYSYAVSLYRPNSLFGLENILFWLALYFLLR
                     YGLRKSLVKTLFALALSAYALMLVLTALGNYMSLDGYLRVQGWADISEFKGFLNYFKL
                     FLNNWATLSVCLLPFPLIAAIHLRANRMAFYGALFVFCMSVLAVLISFSRGAYLSLFV
                     FGILCTGVLLLIAKDAGRRLVLPGLFTGGLLTLILLLFYRPVSNTLAMNKTTSQQRST
                     HGRLNILESGLCKSKIYWLSGTGTHNYPLSPDDCFREEEDSGYSVFTNNLYLQILIEK
                     GLPGFLIYGALFFLVLWRFFINLLRSPEKKDRLTYGFLFAGFAAYAFRELFFSSFFES
                     SQVLALVSLFAAYAADGSPVLFTLRRNSGYSVAAGLLVLVSAWFFIHQLNGRGSERKV
                     EQALVEWKNGNAGKAATLIGHAIKLSPVVFPYHELQGLALAQDSLSIRKLFAGNLQVD
                     RASLSNAKSHFLRALQINPADAGVHFNLGVLEFLQSGSLAGVPASHFIRALEREPNNV
                     EFMVGFGMLAEYSGNAELADRLYKKAVRLDPELIDSEFFSDLKHRNPSSDSLLIGDAV
                     AALRSKIDSSYNMIFLARLARLLMAGEDSAGIKNMLEEVIRNIPTAHRPYFYLGQLAE
                     NGADSSRALDLYRKSFFLGKEDYLAPLALGNYYYSKMVSSPSRSTALSAARYYRLALI
                     TYLEAPSLHHKRAKAQYKVSASIVNDLLFKDLMYYTRARMDFRTIVSRMAESYEKGGD
                     ANMAGHYRKMEGRDLMDVEMR"
BASE COUNT         8033 a         8660 c         7802 g         9016 t
ORIGIN      
        1 accgcttccc gaagctaaga ccttctttta tcagatatca tggaatctga ttactattct
       61 ccttaccttg tgccctatcc gcatggaaat aggaaaaaaa ctgcagacca gccatctttg
      121 ggatcatctt tttgaagaca gccagttgaa aaaagacctg gaagaactgg tgcttccgga
      181 ttatatcatc acttgccgtt ggtttgcggg aaaaagcagc accatcaaac atgtgcgggt
      241 agatcacgcg caccggctcg ccgataaaaa acaggcctat tatatcctga tgctggaagt
      301 ccagtttaag gaagcgttca gccaccaata cctccttccc ctcgccttta ttcccgatga
      361 ggaggaggcc caatacacgg aggttccggt gatttcccgg ctgcgggcgg ataaccggga
      421 agggtttttg atcgacgctt tgtttacggc cgactttcgt aacctgcttt ttgacctcat
      481 ggttaaaaac aaagaaatgc ccctcaacca gggtgtcctg caatttgaaa gttccacttc
      541 ctttaaaccc cccaccggga aaaaagtatt ttctgaaatc ctgaagacgg aacaaagcaa
      601 tacggcggtg atttacaaca accgttattt tttcaaatgg ttcaggcgcc ttttccccga
      661 aagcaatccc gacctggagc tgtcccgttt cctgaacctc aatcatttcc cttatacggc
      721 ccaatatatg ggaagcatca gctggctccg tatggataaa ccccggattt ccctgggcct
      781 gatgcagcaa aaactggata acgaagggga tgcctggaaa tggatgctcc aagaagtaaa
      841 cgcctttttt agcggatttg atcaaaatca tcagctccag ttgacctggg aacccaagga
      901 gaccctgttc gatcccgtaa aacccaaaaa cttatcgccc caaaccaggt cccagcttgg
      961 taaaaaactg atgaaaggag tggtccgtct gggcaaacgt acggcccaga tgcacctggc
     1021 cctggccagg gacaacggcc agacctcttt cacgccggtg gcctttaatc ccgattacag
     1081 cgtatggctc aaaaacaagg tcacctacct gtttgatctc cgaatcggac tgcttgaaaa
     1141 aaatattgga aaactgcgcg gcctggccag ggaatatgga caggttttcc tggaccggcg
     1201 caatgaagtc cttgaagaaa tcctgagttt tgatgaaacc acgctgtgca gcatgcgcac
     1261 ccgcgtgcac ggggattacc acctgggaca ggttttagtc catgaggatg aattttatat
     1321 cctcgatttt gagggcgaac ccgaaagcac catccgggac cgcaaggtaa aacaaagccc
     1381 gctaaaggac gtggcgggca tgcttcgctc ttttcactat gccatttacg ccactctttt
     1441 tgacgaagaa aaaaactgga ccctgcccca acacgacctt tttgacctgg cggaacggtt
     1501 ttaccaactg ctttgcggcc tttacctcca tgggtttatc cgtaccgccg tccggggagg
     1561 cctggacatc ggttaccgtc ccgaaatcca ttacctgctc agatatcacc ttcttgaaaa
     1621 agccgtgtac gaactgggtt atgaactgaa cagccggccg gcctgggcga ttatacctct
     1681 caggggagtg atgcggatca tgggatttta atgcgtcccc ccttacgcct caacctcaac
     1741 cttaacctca accttaacct taaccttatt ctccatttgt ttccatcccg tttttttcta
     1801 ccttgaactt atttcaatga ccgatgtcca cccttcgtca acttgcagcc atcgtctttg
     1861 tcgatattga gggcttcacc gccctgatgc agaggaatga attcctggcc atgcaatggc
     1921 gggaacgttt gaaacggaaa ttgcaggaag aaacggaaaa acatttcgga aaaattcttg
     1981 atttccgggg ggatggggcg ctttgcagtt ttaccagcac caccgaagcg gtgctcgccg
     2041 ccattgccgt ccagatcgag atgcaaaagg agcccgcggt gcctgtccgc atcggcctgc
     2101 ataccggaga tgtggtggtc aatgaaaatt ccatttatgg agacggggtg aatattgcct
     2161 cccggataga atcttttgcc gtaccgggga gtattttcat ttctggcaag gtatatgatg
     2221 atattaaaaa ccaaaaagaa atacaatcgg tttccctggg aaaatttgcc ctgaaaaatg
     2281 tcctggagca gatggaaatc tatgctatca gcaatcccgg aataaaaatt cccggcgaaa
     2341 tcaacctcct gggcaaagga gaaaaaatcc agcccaacca ggtgctggtc ctgccttttg
     2401 taaatatgag caacgaccct gaacaggaat atttcagcga cggccttacc gaagaaatca
     2461 tttcctgcct gtcccaaatc catgaagtaa agatcatttc ccgcaccact tccatgaaat
     2521 acaagggctc caaagaaagc cttaaaaaca ttggaaggga aaccggggcc gcctatgttt
     2581 tggagggatc ggttcggaaa gcagccaatc tccttcgcat tacggcccag tttgtggatg
     2641 cgcaccgcga tacctacctg tggaccgaaa cctaccgggg gaccctggat gatatttttg
     2701 atattcagga aaacgtgtcc gcccgtattg cagaggcgct ccggatcaga ctgacgaaaa
     2761 acgaaaaaga caaccttcgc aaacggtaca ccgatgattc ggaggcttac cagctttacc
     2821 ttcagggccg ttttttcctc aagaaaagaa acgccgcggg catgaaatcc gccatccggc
     2881 actttgaaaa ggccattgaa aaagatcccg attatgccct ggcctgggcc ggactcgccg
     2941 atacctacag cctcatgggc gaatacacca atatcagccg ccgggagctg ctcccaaaac
     3001 aggtgaccgc gctgaacaaa gcgcttgaaa tcgatgaccg gctgggcgag gtccatattt
     3061 ccaaagcgac cgccctgatg ctgaacgact gggattgggt caatgctgaa aaagaattca
     3121 agctgggcat tgaattgagt cccaaatatg ccaccggcca tcactggtat gcggaatggt
     3181 tattgtattc cggtttcccc gatgccgctt ttgaagaaat taacctggcc taccggctgg
     3241 aaccgtcttc gcaggtaatt gtaaaggatc tggggatgta ttattattac acgcgtcaat
     3301 acgaaaaggc cgtggaaatg gccttcaccg ccctcgaact cgacccggag tttgtatctt
     3361 cctaccgcct gctttccctt gcttaccttg ccctggggaa taccgaaaag gccctggaag
     3421 caaatcggca atggggtgaa aaatcactca attggatgaa aacagaattg ggccttgctt
     3481 atatcctggc ttataccggc caggaggaag atgcccggaa gatcctggaa agggaaggat
     3541 tcgaaaatga actgggccac aatgattgcc ggggattggc cctggtttat acggcactcg
     3601 gagaccggga cagggctttt cattggctgg aacaggggat aaaccgccgc gaggagtccc
     3661 tcaccagcat ccagaccgac cccaaatttg accccctgag aaaagatccg cgttatacag
     3721 aagtgctcaa aaaaataggg ctgtaccggt gattcgggga ttgtcccttc ctgctgcctc
     3781 ggtttaatat ccggttcaag gcggctgccc ccgatttaaa ggccagtcca tccctggccg
     3841 gttaaaaaat tgtggacaat tttatcctta aattataagg catttattat ttttaccccg
     3901 ggtcccggtt ttaaggtgtt ttattattca tccttaaaat taaataccca tgtctctcag
     3961 aattcttttg ttttccttcc tgatccctct gacgggggtc ccggcttccg cgcaaccggt
     4021 gaaaaaggac actttaaatc agccaaccat aaaagggtat catattggcg tagtgcagcc
     4081 ccttctggcc atccaccaga acaaaacgag ctacatgacc gaatacgatt tttattcgat
     4141 tggttttccg tttggcctca cgttgcggac ttccggccgt ttcctgattg accttgaact
     4201 ggtccctttt attaaaccct acgcggacga cgccggaaaa ccctatgatg tgcatttatt
     4261 atatcatccc ggcgtactgt atcctttggg gtccgggttt actttgggcc tgcgggcggc
     4321 gtttgaatcc ggtcaggatc aatttggatt caccccgctg atcaataaat cgtttaagat
     4381 cggcccctcg ggttcctttt ttgttgaatt ggtttttccg gggcggttcg ggcctgaaaa
     4441 atcaagcggg tatacccagg ttggcggcat tcatatcggg ttcggctttt agcgctttta
     4501 ataatgaggc cttaggcccg gtgatggatt caagaggcct tcctgggccg gtttaagacg
     4561 gttttttcat ttttcagttc ctttgccaat tgctgaatcg attttccctg aaacatttca
     4621 agcagccggg gtttgatctg tttgtaggtt gaatgcaggg ggcagggatt ctgatcggag
     4681 cactggtcca gccccaggac gcattgggta atggtggttt cttctcccat gagttcaaga
     4741 acgctgaaca cggaccgggt tttggattcc tggttcgtat aaaaaccccc gccgggtccg
     4801 gtgaccgagg ataccaaatt ctggtctttg gtcagtttct gcatgatttt ggcggtaaag
     4861 gacctgggcg acccgatcgc cttcgacagt tcttccagtc cgatcttatg atccggatca
     4921 ctgttttgcg caaggtaaat tattgctctt aaagcatatt ctgtggattt ggaaaacata
     4981 tggataaaga tgaaaaaaaa atcgcaggat cggataatta aagatatttt tatccttaat
     5041 taaaaccgga cccgcttact ttttaattaa aacctttaat tccttgtcaa atgaagacta
     5101 cggaccatca cctcaacggc cttaccaaaa gcatcagtta tttcatgaac acccaaaact
     5161 ggtgggggcc cctcctgttc atttttatca tcagcgtcct gggcgtaggc atgatcggat
     5221 ttcagaccta ccacgacgca ccgccattgt ccgggtttaa gaaccaggaa ggggaaatca
     5281 ttctggacaa ggccgaaatc accaagggtc aactggtttt ccataaatac aaccttatgg
     5341 aatacggcag ttttttcggc gacggggccc agcgggggcc tgatttcact gcagaagcgc
     5401 ttaaccaggt agcgaaaaac atgaaactat attactccgg ggtctaccag gatgaaaaag
     5461 gggtgacgcc cggtgaagag gccattataa atatagaggc ccggattaaa aaggagctga
     5521 aaaaaaacac ttaccggaag gaagaggatt ttatttatgt ttctccggcc ctggctttcg
     5581 ctttaggaaa ggtcagggaa tattacctgg acctcttttt tgaaaacaag gaaggtcgtg
     5641 cttttttgcc caaaaattat attgaaaaca aagaagaggt ggccgccctc ggttccttct
     5701 ttttctgggg agcctgggtc tgtgtcaccg aacgtccggg aaaaacgttt agttacaccc
     5761 acaactggcc ctttgatccg gaagccggaa acaccccgac ctccccggtg atcctgtgga
     5821 gcgtactggg cttgttgggt tttgtactgg cctgcggcat tgttttgtat tttattggcc
     5881 aatacaacca gcttcccaat aagtttttta aacccgcaac ccaggacctt tttaccatgg
     5941 aaagggtcag aaactacacg cccagcagga cgcaaagggc tacttttaag tttttcctgg
     6001 ttgctgccct tttgtttttc cttcaggtat ccagcggcat cattaccata aacgattttg
     6061 taaacttcct gggttatatt ggaattcata tcacccaggg ccttccggtc accattccca
     6121 ggagctggca tctcatgctg gctttgtact ggatatccgc ctgctggatc gcctcttcga
     6181 tttttatcct gcccattctt gcaaaaacag aggtccgcgg ccagctccgt ctgatcaatg
     6241 tattgtttgt cctattattt atcctggtcg gcggctccct cgccggcatg gtgcttggtc
     6301 ccctgaatat gctgggggat tactggcacc tcctgggcag ccagggctgg gaattcgtgg
     6361 actttggtaa aatttaccag gtcctgctca tggtgatttt tgccctttgg gcggtgatta
     6421 tttaccgggg catcaaacct gcttttgtaa aaggccagca ctggaacctc cccaactgga
     6481 tcctctattc ggtgatcggg attcccctgc ttttcctttc cggatttgta gccaaacccg
     6541 aaaccaattt tgtgatcgca gacttttggc gctggatggt tgtgcacatg tgggtggaag
     6601 ccttttttga agtttttatt acggtcatca tcagttacct catggtcctg atgggcctgg
     6661 tcagcaaaca ggccgccatc cgggtggtct attttgccac ccttcttttc ctgggaaccg
     6721 gtttattggg tatatcccat aatttctact ggaacgccaa acccgtggcc accatggccc
     6781 tgggcagcgt attttccacc ttgcagtttg tgcccctcat tctgcttacg gtagaggcct
     6841 ggagatttaa aaatacgccc aagatcgcgg tgggtgaggt ggagcataaa aacctgggtg
     6901 atttcggctt tccagtggtc tttttatttc tggtagcggt caatttctgg aattttttcg
     6961 gggccggggt attgggcatc atcgtcaacc tgcccatcat gaactatttc gaacacggca
     7021 cctacctgac catcaatcat gcacacgctg ccttgatggg ggtatatgga aacatttcgc
     7081 tggcggccct tttatttgcc tccagcctcc ttatcaaagc ggagcagtgg aataaaaaac
     7141 tgctgctggt gtccttctgg tccatcaata tcggcctgat gctgatggtc gtcctcgacc
     7201 tctttcccgc gggagccatt caatttaagg ccgtggtgga aaaaggcttg tggtatgggc
     7261 gctcccatca cttcattgaa aacggaacgt tcatctccct gacctggctc cgggggattg
     7321 gggccagcct gtttttcttc ggaggcgtca ttccattcgc ctggtttatg gtgacccgtt
     7381 ccaggcatct gaaagccgcg gacctaagac aagaattcga aatgaagtcc agcccggaac
     7441 ccagccccga gcctgaactg gaggaagtct attgatcttg tcgcttaaaa ccatctggtt
     7501 ttttacgaac ccggaaaata aaccagcccg tttttaagtt attgaaacaa ggcttgtgtc
     7561 cggaaatgca tctataccag atggttcaac ggcaaaatag gtaagtatga aatttgacta
     7621 cggggtccca gaattttacg tttctgatcc caatataaac cacttcaaca gccctccgga
     7681 tcatccgggg cccaggctga ataaatcaag ccgacggaca tttcatttcc caaaaaatgt
     7741 gcataaaaat gccccggccg atttgcccca ttccgtggac attccttccc ggcacagcct
     7801 cgacccctat ctatgggatc ccggtttcct ctgtgattat gtggtcagga tccatcacga
     7861 ttatattcaa aaagccctgc ctgtactgct tgcaatgggt aaaaaggtca cggtcctcca
     7921 tggcaataca caggtggaaa ccctggccat cctggaacaa atggaaatgc tgttttcaga
     7981 cctgaaactg catttgttta aagaagaaca gcttattttt ccttacatcc ggtccctggt
     8041 ctgccttgaa aaacaaggta aaaccattga tatcaccagg gatggccccc tgtacaagcc
     8101 tatggccggc ctggaagaag atcacgggct ctttgaagcc ggtatggacg ccattcgcga
     8161 cctgagccgt gattttacgg tcccggtaca aaattcgcct gttttcagtt cctggtacgg
     8221 ggcgctccaa gccttccggg aagacctcat ccagcatacc cggctcgagt ccagagtcct
     8281 ttttcccaaa gccattgccc ttcaaagaaa actgaccgcc tgacccggag tcagacggaa
     8341 gcccccctca gtgaggtccc gcgtttgggt gagggaccaa aattttaaca caaaaaagtt
     8401 tcattttttt tccaggttgt taatgatctg gttctgaacc gcgaaggccc ccagccctat
     8461 agtcaaaaag cacggaccgg ggcctccatc cctgtccgga tacattaaaa aaaaatatta
     8521 tattttattt ttagcgaaag atctattgaa tatctttgtt caataaggac aactctgtcc
     8581 tcaatcaaac agtaaataag aaaactatgc tcatattcaa gttagaggat gactttgtgc
     8641 agggctataa gcacctcgat cccggttttg gtttcaatgg actgggcctg ctgacctatt
     8701 tccgcaccta ctcccgggta aagccggatg gcaacaacga acaatggtac gagaccgtac
     8761 gacgcgtagt agaaggggct tacagcctgc aaaaacaaca tattctcagc aatgagctgg
     8821 gctggaaccc aaagaaagcc cagcgctccg cccaggaaat gtatgatcgc attttcaata
     8881 tgaaattcct gcccccggga cgcatgttat gggccctggg tacgcccatt attcatgaaa
     8941 aaaaaatcgg ccaggctctg ttcaactgcg cttttgtttc cacggatgaa atcggcaagt
     9001 ccaccgaaga cgcagtcaaa ccctttttat tcatgatgga catgagtatg gtcggcgtag
     9061 gggtgggatt tgatgtggct ggaggcggaa tgatcaccgt taacaaaccg gaatccgaag
     9121 acgtggacga gtttatgatt cccgattcca gggaaggatg ggtggaaagc ctttcccgcc
     9181 tgctgaccag ttatttttca aaacattttg atgacggggg ggcccatccc gtccggttta
     9241 attatgaaga aatccgggct gccgggctgc cgataaaagg ctttggcgga gtctcctccg
     9301 gccccgagcc cctgatgcgg atgcacgaac agatccgaag catcctggac caaaccgacg
     9361 gccaactgct ttcggtgacc acgatcaccg atatcatgaa cctgatcggg caatgtgtcg
     9421 tcgccggcaa tgtaaggcgc acggcccaga tcgccattgg agatattgaa aacaaagaat
     9481 accgaaaact gaaagattac cgctggaacc cggaatccgg gcaatatgaa ggcagtatgg
     9541 cgcaccgtgc gtcctggggc tgggcctcca acaacagcgt actggtcgac cgcaattccg
     9601 atttcaaacc cattgcggcg cagaccgcga tcaacggtga acccggatat gtcttcatgg
     9661 acaatatcaa agcgtactcc aggctttgtg accctgcgga ttataaagat gaaaaagcca
     9721 aaggcaccaa tccctgcggg gaacagtccc tcgagtccta tgaattgtgc tgcctggtgg
     9781 aaaccttccc gtcccgggcg gactcttatg aagattacat gcgcacgctt aagtttgcct
     9841 atctgctggg caaaacggcc accctcgtca gcaccacctg gcacgaaacc aaccgcgtcc
     9901 aaaaacgcaa ccgcaggatc ggcacttcgg tatcagggat caccaatttt ctggacaaac
     9961 attccctgga gaccctgcgg gtgtggttaa acagcggata ccaggaaatc cagcgctggg
    10021 attcgatcta ttcttcctgg ctctgcgtgc cccggagcat caaaaccacc agcgtaaaac
    10081 ccagcgggac ggtcagcttg ctggccggcg tcaatccagg ttgccatttt cccgaatttg
    10141 aatattatat ccgccgcgta agagttgcta aaaactccac cctggttcct gccatccggg
    10201 aagcccactt gcccattgag gacgatgtgg tggacccttc ctcttacgtg gtggaattcc
    10261 ccatacacaa cccgggcaaa agcctcaaaa aggtttccat ctgggaacag gtagccctcg
    10321 ccgttttcct gcagaaatac tggtcggaca accaggtctc ctgtaccgtg accttcaggc
    10381 cggaagaagc caatatgatc cacgtcgtac tggattattt caaatatgaa ctgaaatcca
    10441 tcagcttcct gccccagctt gaaatgggcg cttacgccca gatgccttat gaaaccatta
    10501 cccgtgaaaa atacctggat atgacggccg gcatcctccc catgcaattt gtggaaatga
    10561 aggaagacgc ggaaatggaa aagtattgcg acggggcggt ctgcctgaga atttgatccg
    10621 gttttttccc tgaagcggat atccagcgcc ttaaatgaag cgctggattt ttttatttat
    10681 aaaatgtaaa aggcctccca cagtctcggg atggtcatgc gcccgaatgg cgcgggctat
    10741 ctttttattc cggaaattcc tatttttatt ttagttcccc tccgttcaga atcctataac
    10801 cccaaaagcc tttatgtttt tccatctttt accttttata cttgcgcttc tgacctcaac
    10861 tggcccgaat gaagaggccc ggcctcaaga ggcctgcctt cccaacggaa tcattttaaa
    10921 caatcagtcg caaatcgatg ctttcccggg actttatccg ggatgtgaca gcatagaagg
    10981 gaatgttttt attaacgggg cggtgtcgaa tctcgattct ctgaaaggcc tgagggccat
    11041 aggaggcagc ctgtttattt acgctacctc gttaaaaaac ttttccggcc tggaaggttt
    11101 gcgttcggtt gggggagaat taagcgtttt ttcaaatttc catctgattt ccttttccgg
    11161 cctggattca ctcaaggaac tgggttcacg actttccatt tatcagaacg attccctgtc
    11221 ggatatcctt ggcttatccg gcttgttgga ccttaaaaat aatccccttc gcatttcctt
    11281 ttgccccagg ctgtcaacct gcctcattcc ggccgtatgc ggccacctgg cctcgggagg
    11341 ttccgccgtc atccacgaca atgccccggg atgtaattcc aggttccaga tcgccaaagc
    11401 ctgtgatccg ggcgcccttt gtccttcggt aaatatcgag atccatggcc aggaagattt
    11461 aaatgacttc atcagcctgt atccggaatg cgaggacatc gcgggttctt taaatcttat
    11521 ttccgttgag gacacttttt attctttact ccccctggcc ggtatccgca ccattgggaa
    11581 tgtactgaat atcgtaagca atccttttct ttctgattta aaaggtctcg attccctctt
    11641 atccgttgct aacggattga ccattcacag caacccccgg ctgaaaaacc tggatgggct
    11701 tgggtccctg caatgggtcg gaaatacttt tcagctcgtg ttgaacgatt ccttatcctc
    11761 cctcgaaggg cttgggcaga ttgatcccga cagcctgcat accttatatt ttcagttctg
    11821 ccccttgctt tcggcctgcg atcatgcctc catctgccaa tttctgagtg gcgggggcct
    11881 tgcttacctg gatggaaatg cacaggactg caacacctca caccggatac aaaagcattg
    11941 ttatccggac agcctttgtc ctccgggaaa cctgtatttc agaacccagg aagaagtgaa
    12001 tgatttccat atcctgtatc ccaactgcca agacctggcc ggaaacctca gcatcgatgg
    12061 caggaatatt aacaggctgg attcgttatt tggcttgaaa tcggtcgccg gtcaatttta
    12121 cctgtccaac aacgattccc tcgaacattt aaatggattg tcgggtcttc aatccattgg
    12181 tcagaatttc tatattctgc ttaatccggc cttgctttcc ctggccggac tggaatccct
    12241 gcgtcagatc ggggtcggcc tggtcatgga caataatccg ttgctgcagg atttgagtgc
    12301 cctggccgat tcgctccttg gaacaaatcc cgatttttat cttcggatat tctacaaccc
    12361 ggcgctggcg ctttgtcatg ttccggccat ttgtaattat gtaaaccagg ggggtgaagc
    12421 cgatattgaa ggaaacgctc cgggatgtac cagcctcaat gaaattattc tgaattgcaa
    12481 tcccagccag gactgccctc cggggaatat tgagatcagg agccaggcag acctggattt
    12541 tttcaccctg aactatccgg attgcgatca cattgccggt tccctgacta ttaattccga
    12601 acagggcccc gtcacctccc tggcctcgct cagtcatttg aaaagcattg ggggttcctt
    12661 ctacatagaa aacaatccgg cgcttgccag cctttccggc ctggactccc tggagtttgt
    12721 cggcgaagcg ctgagtattg gttccaacgc cggcctgcag agcctcgaag gcctggaatc
    12781 tctccggatc ataggagatt atctggccat ttaccttaac gacgcactac tcaggctgga
    12841 gggcctggaa aacctgaacc ccgacagcct tgactattta tatgtttacg aatgttcaca
    12901 actttcagaa tgtaatcttc ccggtttctg ttccttccta atcgaagggg gtattgccgt
    12961 catccgggac aatgctccgg gatgtgataa tccatttcag gtcattaaat catgccagcc
    13021 cggcgagatt tgccctccgg gccaccaggc gatttacaat caacagcaac tggatgaatg
    13081 ggtggctgct tatccggatt gcactgacct gccgggttcc ctgcagatcg ggccggccga
    13141 ggaggaagac cccatccttt ccctcaccgg cctcgctaaa ctcggaacga tcggcggcac
    13201 ccttgaaata aatcagaacc gctggctcaa aagccttcag ggtttggact cccttcaagg
    13261 ggtgggcgcg gatctccgga tttattccat ggacagcctg gaaaatcttt tacccttaaa
    13321 atccctcaaa ctcatcgaag gtcagttata tatcagggac aaccctgtgc tgaccagtct
    13381 ggatggtttg gatcagtttg atgcggacaa actggatgcc cttttcatct ggtacaatga
    13441 acaattgagt ttctgttcaa atccggccgt ttgcgcttat ctgtcaacgg gaaaatctgc
    13501 cgttttgtct gagaatgcac ccggatgctc cagtctcaat gaggtgaggc ccggatgtgg
    13561 cgtatcttgt tcgcaggact tttttgagtt ttacagccag gatgaaatcg atgcttttcc
    13621 tgccaataat ccaggctgcc agaacattgc cggttatgta tttatttcag ggtcagacat
    13681 caccaacctg gattcgctga ggcaaatcaa aagcatcgga caggatatta ccattttcag
    13741 taaccccgta ttgaatgata tttccggtct tatgaacctg acttcggtgg gatcgtatgt
    13801 ggacatcagt ttcaatccgc ttttgacgac cctggatggc ttatccggac ttcggtccat
    13861 cggaggctat ctgggcattt acagcaataa tatactggac aatatcgatg ccctgtacca
    13921 ggtggaccat ggggggatcc aggaactcta tctgcagttt aaccccaggc tgcaggcctg
    13981 caatgcggaa gtggtttgcg cctttttgag cgatgaaacc aaatccggcc tggcttatat
    14041 caatgacaac aaacccggtt gcaacaaccc ttctgaggta aagacaaact gtgggctggc
    14101 caactgccct gtgggtaacc ttacgcttag cagccagtca gaggtggatg gtttcagaag
    14161 cgcttacggg gattgtgccg ccctgcccgg cgacctgatc ataaacggaa cggacattgt
    14221 caacctggat tccctctcca atctctacca aatcggcggc caactgatca ttgaaaacac
    14281 cgggctcgaa aatttgcatg gccttgacca tatcctgtat actacgatca gccatttgtc
    14341 aatccggaat aatccctctt tggccgtttg tgatgtacgc gccatctgtg attttctttc
    14401 cctgagtggc gggacttttg aaatcatcgg caatgcgcaa ggctgcgcca cggaatcgga
    14461 agtcaggtac tcctgttcga aaacctgttt ttgggacggg gtgggtttta cctcccaggc
    14521 ccaggtcgac tctttccgga ggctatacgg aaattgcgaa gtaatccccc atgcggtgac
    14581 catttccggc tcaggcatca atagactgga ttccctattt aatcttaaaa agatcggccg
    14641 cggcctcacc gtcctgagca cttcggttcc cgacctggat ggcctggctg gcctggattc
    14701 ggtgggggga agcctggaca tccggagcaa cagccagctt cttaacatca gccagcttca
    14761 aaatatccgg tccattggca gcaccctccg gattcaaagc aatgctaagc tcacttcact
    14821 cgccggcctt gggcgccttt ccggcgtgcc cttcaacctt accgttcaat ccaattccgc
    14881 ccttgccagt ttatccggcc tggattcaat cggatatgtc ggaaataccc tcctggtcat
    14941 gaacaatccc cagctgaact ccttttccgg tctccgttcg cttaaaaccg tggggttaga
    15001 ttgccggctt gaaaccaata acctggtaat gaatttccag ggattggaaa aattggaaag
    15061 cgtgggaaat gatctcagaa tcctcaataa caccggactt caaaccaccg tcggcttgtc
    15121 aggactgaag acgataggcc gtcatttgcg cattgaaaat aacgggggcc tggacagcct
    15181 gtccggtttg tcggccctgg aatccatagg gagcactttt cagctaatca acaatgccag
    15241 cctgaaccat atacacgacc ttagtagcct caggcacatc ggccaatccc tgatcatacg
    15301 aaacaacagc ttattgtcaa gccttaacgg gctggaccag gtggaagctg atcccctgga
    15361 ctacctgagc atcgaaaaca acccgtttct tgatttatgt aatgtgcgga gcgtctgcct
    15421 ctaccttttt gacccggaca aaacctattc tatttttgga aatgccatat cctgtcagac
    15481 gggggaccag atccttgctt cctgttccat tgccttaccg gtatcgctga tttcatttac
    15541 agccagcctg gagcagggcc aggccttgct tcaatggaat accaccggtg acctgaggcc
    15601 cgatcttttt atgattgaac acagccggga cggcgagcgg ttttccctcc tggataaaat
    15661 gcccttcggg caccaatccc atccgtatac ctatcgccac tctcccccgc cgggtatgaa
    15721 ttattaccgg gtcaaggcgg ttgctgaagg cagaagttcc gtgtattcca gtataggcag
    15781 cgtccagatc aaaaacgaaa attgggtcct tcccaaccct acccgcggga tattacatat
    15841 caggcctccg gatgagcaaa gatgccggat tgaagtcagg gaccttaccg ggcgcgtatt
    15901 aatgaatgtt gccggacagg ggccgcaaat aatggatctt tccggccagc cctctgggat
    15961 atacttgatc tccgttttga gttattccgg gcgccgcatc ttcaaggtat taaaagaata
    16021 aacactccgg attaaaaaac gtaatttatt acatccttgt cggtgggatc ctgatgatca
    16081 atctgatcaa tttgctggat tccaccgatt tctgaggagt tgatttatct atggattttg
    16141 gatttgcttt ttggtttgat ccatatctcc aaacaatttc ctgcccatat aaagagaggc
    16201 gaggaggatg gcaatggaca cataacctac ccaatcgtaa cgcagtaatt ttccattcaa
    16261 tcccttgata acaatcgcgc cggataaaaa ggaagcggcc gaagtgccca attgttggac
    16321 actgctgttg aagctcataa aactgccccg ctgggccggc aatggtacat tgctcagcat
    16381 ggcctgggcg gtcactcccc ggcccgtagc caccataaac cataaaccaa agaaaaacag
    16441 gaccagggga aagggccagt cgggaagatt agtgatgatg aataccacga ccatgcaggc
    16501 tgccaccgac caggtaaaca ccctgacttt gcccgcgtgg tcagccagtc tgcccaggag
    16561 gaaggaagaa aaaaaagccg aaagaccacc caccaggtac acggtcggag tgagggcctt
    16621 ggagaatcct ttattgaact caaggtatgg attcacaaaa gggatgatca taaaatggcc
    16681 catcatgatc agggcggaaa acatcagggc attgcgctgg tacttattgt ggatgacctc
    16741 gttcagaatt ttcaatttgg acgccggatt ttcctgattc acgtgacctt ccatttccgg
    16801 aatgtattta ttcaataggg gaatgatggc gatgccgagg atgccgacaa ataaaaaggg
    16861 ggcatgccag ctgatcgcat tggccaaata aagggaaaag ggcacgccca gggtggatgc
    16921 aatggcaaaa gaactcatta cggcgcccat ggccaatccc cttcgttcgt acccgaaaat
    16981 atccgaaatg atggaaagga cctgggcccc gatcagcccg ccaaacaaac cggccagcac
    17041 ccgggccgcc atgagaagat taaaaccggg agcaagcccg caggcaatcg tcccggccag
    17101 gaaaccggcg aaagcataga tcagaatttt tttccgtcca tgattatcca caaaaaaagc
    17161 cgcggcaaaa ccggagaagg ctgccgtcag ggtataggcc ccgaccagca ggctgaactg
    17221 ggccggactg atgtcaaaat aaggcatcag gtagttcccc aggggcatca tgatcataaa
    17281 atccaggata tgcgtaaaat tgaccagggc gagtaaaaaa acgatagcgc gttcatctct
    17341 ttgcatgtgc aaatatgcgg attcggtttt gcgccctgat ctgccctttt gtcaacgaat
    17401 tgaaaatgcg gtaagacggt caatgacagg ggaaggccgg gtactgactt tttgaattgt
    17461 caaaaaacga tcaaaaaccg ggatattctt aaattgtggc gcaatccgca tcatgctcaa
    17521 aaaatatttc tcattcaatc actggatcgt cctggggatc atcatcctgg ttgaattgat
    17581 cgtaaatctt ctcctcaagg ccaccctgct taccgaaaaa gtcctttata attctctggt
    17641 cgaaaaaatg actctggagg atatcagttc cttttcctcc acggtgcgct ccagcgcctg
    17701 gatgctggtt gtctttggag tagcccagcg catcggggag atccttttga tctcggtttg
    17761 catcagcatc ggaaccctgc ttttccgtta cgatatccag ttccgtcaga ttttgaaggt
    17821 ggtcatcctt tctttcctgg tttttacggt ttccaggctc cccttgttga tcaccttgct
    17881 ttccggcaag gtcgaaaaat ttgaagacct ttcctttatg cccaatcttt cccttgcgga
    17941 ttggttccgg cccgagaacc tgcctgaatg gggcctctac cctttgcagt tgattaatgt
    18001 gtatcagttt ctattcatcc tgctgatggc cgccggattc aatcggatcg atccccgggg
    18061 aatacccaaa tggctgggcc tgatcctgct cacctacggc accggcctgg ccattaccgt
    18121 ggtgctgacc gcttttgcca ccgctatttg atttgtattt cccctgctca agtatttgat
    18181 tttcaataat taacacattt tttatttttg tttaactaaa attgtggtat ttcaatttaa
    18241 cttttttaga tttgtcctga tacaagcccc cttttacttt aagtctgatt tttaattttt
    18301 tgtcaacctg aaaatattcc ttcaatcata acaaaaaaac ttgtgtttat gaaacaactt
    18361 tccttgaaca tgatggcaaa cctcgagggc gggggcgctc ctattgctga aaacagggcc
    18421 tgtttatcgg cctgcgcctt gtactccttt gcttgtgcca acggcctcgg ctttctcaaa
    18481 aggctggcca gccgcctcat cgatgtctat tgcaaaggct gccattgccc ggataccacc
    18541 atgtgatttc attattcagg ttttatttaa atagtaacct gaccaaaatt ttatttaatt
    18601 caccataaat actcgagttt tatgaaacaa ctttccttga acaaaatggc ttccatcgaa
    18661 gggggtgctg cgttggagaa cagggcctgt ctttcagcct gcgccttgta ctcctttgcc
    18721 tgtgccaacg gcctcggctt cctcaaaagg ctggccagcc gcctcatcga tgtctattgc
    18781 aaaggctgcc attgctcgga taccgtttag ttaaggtaac cgaattaaaa aatgcccggt
    18841 gatgcccttg aggtatcccg ggtttttttt gctgattatt gaacaattct tatttttatt
    18901 actcccctcc ggcaaagacc aggttttaaa ttttgattaa tcaccataaa actcgagttt
    18961 atgaaacaac tttccttgaa caaaatggca agtctcgaag ggggttcttc cacggcggag
    19021 aacagggcct gtctttcagc ctgcactctg tactcctttg cctgcgccaa cggcctcggc
    19081 tttctcaaaa ggctggccag ccgcctcatc gatgtctatt gcaaaggctg ccattgcccg
    19141 gatgccttct agattttggt aaccgaatca aaaaatgccc ggtgatgcct ttagttatcc
    19201 cgggtttttt tgttctttta ttgaacaatt attactttta taactccccc tgcaaacgac
    19261 aggatttaaa ttcttattaa taaccataaa acccgagttt atgaaacaac tttccttaaa
    19321 caaaatggct tccattgaag ggggtatagg caccgaaaac agagcctgtc tttcggcttg
    19381 tgccctgtat tcttttgcct gcgccaatgg tcttggcttt ctcaaaaggt tggccagccg
    19441 cctgatcgac gtctactgca ccggatgcgg ttgtacagat tctataaaat aataaacaac
    19501 cgtttacaaa aaacccgaga tgcctgcgtg ggtatcccgg gctttttatt taaagcccct
    19561 ttcttatgaa ttttttaatc agctggttta cgctttaaat caattattat tatcttcaac
    19621 tttccttcca gccagaactt tattccaaat gtcatcattt attcatctta atactcgagc
    19681 ttatgaaaca actttccttg aacaaaatgg cgatcctgga aggaggcgga ggcagctctg
    19741 aaaacagggc ctgttactca gcctgcgccc tgtactcttt tgcctgcgcc aatggccttg
    19801 gcttccttaa acgattggcc agccgcctga tcgatgtcta ctgcaccgga tgcggttgca
    19861 attttgacca tacctaacgg acgtttcaca gaataaagac ccggagtatt tcatttatat
    19921 tccgggtttt ttatttaccg ggttttgttc acccgggcct tcgggaacaa atccaatccc
    19981 tgcttcagaa actcaacttc ctttctccaa accagtaagg gatttttaca tttgtccgca
    20041 gatattctta agtattgagc caaaaccagc aatttcacgt tcagcaacac gaccaatcgg
    20101 actgcggggt ggcctgtctg ctttctgttc ttcgttcgtt cggaggaaac ctgcccctga
    20161 gcaccctcag ggaatggagt gggaccacga taaccgggac gaccatgctt ggcctttacc
    20221 aggccgcgct caagctgggt atggatgccc agggatttga agcggacacc gaacacctca
    20281 agactcttga acatcccgcc attctgcatg ttcaaacccg ggaaggcacc cttcactata
    20341 tggtttgtta tgcctggtat ccagacatca gccagtttga aatcagcgat cccgccgaat
    20401 acggtataaa attttatagc ccgaaagacc tggacgcgct ctggcagtcc aaggccttgc
    20461 tggtcctcaa atccacgccg gaagtcaaaa aagtatccag gaataattct tttattcaaa
    20521 aaatgcggtg gttgtatgga tttgcccggc cggattttga ccttctcctg gtttcgttct
    20581 ttattggcgt atttgtttcc ctgctgggct tatcggtagc cattttttcc cagaagctca
    20641 ttgatcagat cctcccggaa aaaaatctga cgcgactttt tgtcggatcc ggccttcttc
    20701 ttttcctcct tttgcttcgc tccggaatcg gttacatccg tcagttgttt ctccttcgtc
    20761 agacccgaga ttttaatgtg cgcatcatcc gcttttttta ttcttcgctg ctgagccttc
    20821 caaaaccctt tttcgacacc cgcaaaaccg gggagctcat ctccaggatg aacgacaccg
    20881 cacggatcca gcaaacggtg tccaatgtat tcaccagttt taccattgaa gccgtggtca
    20941 tcctcatcac taccattgcc cttatgtttt atcaccttca acttgggctt ttatccttgt
    21001 tgtggttacc ggtttttggc ttgatcgttt accggtacaa taaacccctg atcgaaggcc
    21061 agcaaaaagt catgtcgact tatgccctga acgagagcaa ctatatcgat accatccagg
    21121 gagtgggggt gatcaaactc gccaatgcgg aagcccgttt taccgaacaa accctgggcg
    21181 tttaccatgt ttttcaggaa aaacgttttg aacttggaca gctggtcaac cggttcggaa
    21241 cctattccca gatcgccggt tccgtattta ttgttggcct gatcgtattc agttccctgc
    21301 tggtgcttcg caattcgata tccattggaa ccgtgatggc ggtcatccaa ctcatcggga
    21361 tggccatggc ctccgcctct tccatagccc tgatcaatat tgttttacag gaagccaaag
    21421 ttgccctgga ccgcatgcag gaatttaccg ccctgccccc tgaatacgat cccctggaag
    21481 aaaaggaaaa acgaactctg gtttcttttg attcccttga aatcaccgat ctcgacttca
    21541 gatttaccgg tcgccccctt ttgctggatc acctgagttt taatatcaaa cggggcgaaa
    21601 tcatcgccat actcggagaa agcggttccg gaaaaagcac cctactgcaa attatccagc
    21661 gcttttatct ccctgagagc gggaccctac tggtaaacgg cgttcccctt catgaattct
    21721 ccattccgga atggcgcaaa atcatcggcg tcgttcccca ggatgtaaaa ctttttaatg
    21781 cttcactgct ggaaaacatt ctgttgcgtc ccgtggaaga agcggatatg gctccccttg
    21841 gctccttttt tgaagaacat ggtttcaaca aacctttttc caaacttgcc gggggttacc
    21901 acactctgtt gggagaagaa ggaataaata tttccggggg ccagcagcaa ctggtcgcat
    21961 tggccagggc cctttaccac cggccgcaat tgcttctgct cgatgaagcg acttcggcca
    22021 tggaccgatt cacggaacaa accatactga aattattgct tgccatccgt ccccatacct
    22081 ccaccatcat catcacccac cggcccaaaa ccgcttcgat agcagaccgg atctatatcc
    22141 tggaaaacgg ccaattcagc gttagcggct cgcccgatga acttctgaaa tcaaaaaatt
    22201 tttacagcga agcactcatt taataattaa gggaattaaa aattaaggaa ttaagagggt
    22261 ccttcgttta attattaatt cttaattatt ttaattcttt ccatcaccac cacacctgtt
    22321 gccctccctc ataggtaaat attctttcct ccttgctgtt gcagcccctg caattcaacc
    22381 aatacagtcc atttcccggt atttgtttaa actcaagggc gaaatccttt gcggtttggg
    22441 taccggcgga tatccatttg tccttccagt aaaacaattc atagcttgcc ccttcccgga
    22501 tggcgttggc gtccgtagcc ggaaggtatc tgatcttttc gagcactatg ggcctgccga
    22561 aatccaggcc ggcccagtga gcctttgtgc tgtccgtttt catttttatg gtccgaccgg
    22621 gaaatccatc aaaggcaagt tcgggacggg gtatgtttcc tatgggcttt ccggacaaag
    22681 gccccgactg gccgggggta taccattcca tttcccccaa cctgaacgtg aggtttttat
    22741 caaaaatgat cctggcatat cggaaagagt cgcttaattg caggtctacc tgattccata
    22801 ccgattcata cccggccctg tctttaagtg aaagatcgtt ttgtaccctg ggaaggtaaa
    22861 cggttttcgc tttgggcacc tggtacaaca agaccgggtt tgtaattatt gaatcccggc
    22921 cggcttcgaa acgggccccc tccatcgtgg attccaggaa ttgtttccat cgccgcttaa
    22981 acggaaactt ccggtacagg atcagatctt tggttttttc cggatcaatc gtgatcagct
    23041 gctgacctcc actggccgga agcaaaacag gagatcccgc cgccaaatac ctgtggtttt
    23101 tatataacat gggcatgtaa accagtcccc ttcccatgtt tgaaaattca acttcgtcat
    23161 tccggatcat tgctcccgcg atggccaccc actccctcag tttaaatacg cagagataaa
    23221 ccggggtgcc gtcccttcct tttatattca accggatgtc gccgacccgg gtataactct
    23281 gggttacgtc ctggactctt ggatcggaaa caaactccgg caagttcctg atgcctgatg
    23341 tccttgcctt ggccgcaaaa ctggattcca cccatcccag cctgcggcga taaactttgg
    23401 cgaatttgtg atcgccttcc cgcagataag acatttctcc aggttccgtt tctccccctt
    23461 caaagggcag gctctccgag tctcccagga tcagcgcgtc ccatttgtgc gtatcgttta
    23521 catttccgta ttgcgggaca taatcaatgg ccaccggtat tccgagagct cttgccgaat
    23581 aggctcccaa actcgccatt tcaatgcagt ctcccttttt cccaagcagg atctgcccga
    23641 tgctcagggc ggaaggatat tcgcccagcg ttcttgaaaa ttcaaaccag gtcaactgtt
    23701 cattcacgag ggcgcaggtg acctttacca tggattgacc attggtggaa tcgtccaata
    23761 tatgccgata ccgggccctt aacccgcttc gccagtcttc agcgtattca ctataacatt
    23821 tatacggcaa aatataattg cagaaggcat caaagctgac ctggtttttc cagggagcgt
    23881 tttgccaggc tgcaaaggct gcttccgtat ttttgatcaa gaactcggag gccagatgtt
    23941 tcaagtctcc ttttcggctc agttttttca tctgcaaggg tccgattagc cgttccaggc
    24001 ttttccgtac agaatccgtt tgggcattag aatagtcccc aatctgtgga taaaacaagg
    24061 tatcaaaacg ttgcagccat tctccttcat attggtacag ttcctccata ttgctgatta
    24121 aaaacaggat cgcctgcttt ttgaggctgt cagccggatt tgtggaaaaa tgaaggatag
    24181 ctttttccag ttctgggcgg ttggccccgg ccattttcag attacgctcc aaggcatttt
    24241 gatttccggg cttccaggta caggtatata gggccgtcaa taaaaaaaat acggtgcagc
    24301 ggaaaagctg gatggttccg gatcccgatc gaaaaatagt tgtcaatccc tcattgagtt
    24361 ttctcactca caaagaaaat aaccggttga cgaatattgg tatatcccct ccttaattct
    24421 taattcttaa ttcttaattc ttaatttacc ttcaccacca catctgtttc ccgttttcat
    24481 acgtaaacgg cctttcttcc ctgtttccgc agtcccggca gcgaagccag tagatagttc
    24541 ctgcgggtat gccggcgtat tccaaagaaa ctcctgtagc aatttgcgtt cccgccgatt
    24601 tccagcggcc atcccaataa tacaattcat attttttccc tggttcaatg ctgttgggat
    24661 catctgccgg cagatacctg agtttgccca ccggaatcct ccttcccagg tccaatccca
    24721 cccaggctga atccatttgg aggctatccg gtttaatgct tcgtcccggg accccgtcaa
    24781 aagcaaattc agggtgcgga agatttccca ttgggatccc tttgagggga ttcccggttg
    24841 cagattcata tacttccaat tcgcccaaac gcgggggggc gtccttagga aacagaaatc
    24901 gtataaagcg gaaggagtcc gggtccggga taaaatcttc cctccataga gaatcataag
    24961 acgactgatc cttaagggta agatctcttt gataacgggg gcgataacgt ccggcaatgg
    25021 gcggtatttc atgcaacaat accgggtcat taaattccgg agtatgactt ccttccaacc
    25081 tcgaatgata caaaggatgc tgcatataat tatatatacg ggctcttttc aggggattct
    25141 tccggtacaa taccaggttt tggccctggt gggagtgggc tttcaattcc tgcacggggg
    25201 cgtttattct gtgcaagacc ggcggtccgg cggctttgta cgtcctgttc tcataataca
    25261 tgggcatata aagcgcttcc aatcccatgt ctgtgaacaa gagggaatgg ttgtgaacca
    25321 atgcgcccga aaccgctttc caggatcccg gttcaaaaac acagagatag accggggtat
    25381 tctcctttgc ctcaatagta aagcggatat cccgcacttg ggtataaaac cgcgtcacat
    25441 cactgatcct gggattactt aacctttcag gaatatccct gatccccgcc atcctgctcc
    25501 tggctgcaaa agaggcgctg tcataggcaa gtttatgcct gtacgctttt gcaattttac
    25561 cttctccttc gctgatactg cggtaatctc cgggaaaacc ttctacgccg ataaaaggcc
    25621 aaaatgcgcc ttcgggcaaa atcagggcat tccacatatg ggcccctgca aaattggccc
    25681 attgaggggt ataatcaatg gccaccggaa ttcccaaggc tcttgccgaa taggcggcca
    25741 ggttggacat ttccacgcaa ttgccctttt tcccgtttaa tatctggaaa atgcttaggg
    25801 gcgccggata gtctgtaaaa acctctgaat aaccaaacca acccccttct tctttaacca
    25861 gggcacaaca aatgtcctcc atggcatccg gaatctccgg atcatccagg agatactggt
    25921 atttttttcg aagcgttgac cgccagtttt cagggggttc tgagaacgac ttgtacggca
    25981 ggatataatc acaaaaggcc tcaaaaccca cttcggcgcg ccagggcgca ttttgccagg
    26041 cttcaaaagc ttcatcaata ttccggatca ggtagtccgc ggtgagatgt tttaaatcat
    26101 aaagcatttc gacttctcct tcattcgagc ggccgatttt ttcctctatc gaatctttaa
    26161 gtctttggat ctcctcgttt tccacacccg ccgtgtgatt aaagatgggg tcaaactgtt
    26221 caatccattt cccgcttggg tgtttgtggc cttccatatt gctgatcagg aaaacggccg
    26281 ctttgaattt caggctgtct tccgggtgac gggcataccg ggcaagcacc ttttccattt
    26341 ccggacgatt tttcccggaa aaaacaagat tccgctccag cgcgttttga ttcccttgtt
    26401 ccattcgggt gcatgcgccc agcatcaata aaataaacaa tgaaaaaagt ccaggctggc
    26461 ccggtcccgc gtttctggac agaattcttt gcccgccata aaaccggata tggataattt
    26521 tcatagtgtg ataatacatg cccgggaatt taaaagactt tattcatccg tattccttta
    26581 attcttaatt ctcttaattc ttaattccct ttaattcctt taattttttc ttcaccacca
    26641 tacctgtttt ccgttttcat aagtgaacgg acgctcctcc gtgttttcgc aattgtaaca
    26701 ccggagaaga tataaacctt cccctaggac ctgatcaaaa acaagggaag ggctactggc
    26761 cttttgtttt ccggccgact tccatttgtc tttccaataa aacaattcat agatcctgcc
    26821 ggcctccact tcatgactgt ccccggcagc aatatacctg attttatcaa tctgctttgc
    26881 ttcgccaagg tcaaggccca cccaatagct ttttgaggtg tcttcctgtt taatgctccg
    26941 cccgggcact ccgtcaaagg ccagcgaggc gtccggtata ttgcccatcg gtttcccttt
    27001 gagcgcctga gggccctggg aataaaattc cagttcgccc aatttcaggg gtttcccctt
    27061 ttgaaatacc agccggataa attgaaccgg gccggtacgc gccgggacaa cctgtttcca
    27121 gatggaatcg tactcaaagc ggtccttcag gtccaggttg ctgtaaacgc tgctccggta
    27181 tatttttcga atccgtttaa tttcatgcaa caaaacggga tttgtaaaag cggaatccct
    27241 tccgccttca aaccgggcgg aaatcatcgg ggcctccagg atccactcca tccatctttt
    27301 aaaagagtac cggcgggtca ggaccatggt ttgaagctgg tcttcccggg ctacgagttc
    27361 ccgtgggctg cccttcaccg gcactacgac tgcgggtcct gcagcttcat attttccctt
    27421 tttgaaaacc atcgggatat atacttctct ttgtccggac tgcctgaata tagcacggtg
    27481 gtttgaaatg aattcaccgg ctaccggttc aaagccgtgt ttgcggtaaa cacataaata
    27541 aaccgggtct ccgtcctttc cattagcgcg cacctcaaga tctgtgacaa gggtatactg
    27601 ggaagtcacg tccgtaatgc gcgggcttct gaaaaattcg gggatatttt ttattccggc
    27661 ctcgccggct ttcgctccaa aactttcggg gaccctggcc aattgtttgc ggtacacttt
    27721 tgtgaatttg gaatccgcct cccggagaag gcttaggtct cccggacggt ccccggcccc
    27781 ggaataggct tcaaaatttt cgtcggacag gatcagggca ttccagtgat ggccgtccac
    27841 ataatcgatg gtcgccggaa tgccaaatgc cctggcggcc tgggccccga gattggccat
    27901 ttcgacgcag tccccttttt taattttgag gatctgttgc atattcaaag cccccgggta
    27961 atccagttct tcactgtatt tcatccaggt gatttggtcg tcgatctggg cgcagaccag
    28021 atcttccatg gtatggggga tcagagaatc cttcagcagg tactggtatt tgtcgtacag
    28081 gacgctttta tcgtcctgag gaaattcatt atagttgaaa taaggaagga tataattgca
    28141 aaaggcatta aagctgacct cggatttcca tggcgcgttt tgccaggatg aatacgcctg
    28201 gtcgatgttc cggatcaggt aatcggaagt caggtattgc aaatccagtt tttcccggag
    28261 gtcttttttc tccgggtttc caatcacgga cttaatggaa tcgctgaatg attgtatttg
    28321 ttcgttatcc agccaggctg ccttttcaga gaacatggaa tcataggtcc tcagccattc
    28381 gctgtcgtaa tgaaccttgt cttccaggtt gctcaggaga aatacggctg ccttccattt
    28441 caagctgtcg ccgggatccc ggcggtaatg attaagtact ttttcaagtt cggggcgatt
    28501 tttccccgac agggccaggt ttgtttcaag ggcatcccct attttcttct tgtccgggag
    28561 acaggaccca atgaagaaaa aaacaaataa aatgagaccg ggctgaaatt taaaccaacc
    28621 gttcacatgc acaaaataac ggtttaatca ttaccaccat acctggcggt ttttttcata
    28681 agtaaacggt ctttcctcag tagaactgca gtctgcacag tgcaaccaaa acaagaccgc
    28741 cccgggaggg gccttaaagt gaatacaatg tgtttccgct ttctgaatcc ccaaagaaat
    28801 ccatttttcc ttccagtaat acaattgata ttgtttcccc ggttgtacct ggttttggtc
    28861 gttggccggc agataacgga tttgagctat gggcttgctt tctccgaggt ccagccccac
    28921 ccatcgttcg gtatccactg catcttcttt aattatgcta tatcccggca gaccgtcaaa
    28981 ggcgcgttcc ggatggggta ccgtaccgat ggatttccct ttgaggggca ggctgtcgtc
    29041 cggactataa aattcgagtt cgcccaattt aaaggcattt ccttcattgg ctaccatccg
    29101 gacataacga tattttttta tgggattcag ccgggtccct tcccacaaca tatggtattc
    29161 aaggcggtca cgcgccggtt tcccgccgat agtattggtc gcaaaccagg tatagggttc
    29221 tggcgcctta tagatcaggt agggattttt aaactcgggc tcatccgatc cttcaaaccg
    29281 ggcatgaatg agatactggg cataggtcca tttggttctg aactttttaa gcggatattt
    29341 tctttcgagc cgaaggatgg tgggttcttt tcggtccgca accaggtgcc ggaatacccc
    29401 ggtttcatcc acatgcaggg gaggagccgc ctggcggtaa tcgttatttt ggtaaaacat
    29461 gggcatatac acgatgtccc gtcccatatt tacaaaaagg accttatcgt cttcaataaa
    29521 accgccggca acggcattcc attggccttg tttaaaaata cataagtaaa ccggggtgcc
    29581 ttctttacct cttactgcga tgctgcacga ggccgtggga acataggagc cggtgacgtc
    29641 caggatcctc ggatttttca ggtagggggg cagcgcttca attcctctga tttttgcagc
    29701 cctggcgaaa gacgtttcca cctctgcctg catcaatcga tagatcctgg ccgttttggt
    29761 ttcgcccgtg ctgaccccgt taaaatctcc cggcgctccc tccgctccaa ggaagggtat
    29821 gaaactgctg tcggaagtga tgagcgcatt ccaggtatgg cctgtcgtat gattggccca
    29881 ctgcggagtg aagtccaggg caaccggaat accccatgcc cttgccgaat aagcggcaag
    29941 attggccatt tcgacgcaag cgccctgttt ggctttgaat atatcggata tgcggagcgt
    30001 ggaaggatag tcaaaaaaaa cttctgaata ccggaaccag cttttttctt cttccacaaa
    30061 tgcgcaacaa taatcttcca tggttttgag atcctccctg cggagcacaa agtccccgta
    30121 cttttccttt aaccaggctc tccaggactc aggatattcc gtcagggaat tatagggcag
    30181 gataaaatgg caaaaggcgt cataggacac caaatccttc cagggcgcgt tttcccatgc
    30241 ctcatgggcc tgttctatgt tttgtatcag ataatccgcg ctgaggtgtt gaaggtcgtg
    30301 cttcatccgg aggtccgctt ttaccgaagg gggggtatgt cttctaaagg aatccagcca
    30361 ttgcaggatc ccctcccggt ccatttcacc tgccctggaa aaaatgctat cctgcttttc
    30421 aatccatctc cctccgtagt gcaccttatg cttcatattg gtgatcagga aaatggccga
    30481 cttttttttc aggctgtctg ccgctatcct tgaaaaatga tcaatgactt tttcaagttc
    30541 gggacggtta tcgcccgcgt aaaccatgct caattccaat acatctccgg caggagttgg
    30601 ctgctcatta catgaaacca ttcccgcggc cagcatgcag gccagaagac ccgtacccat
    30661 cgctttggct atattccaga tcttgccttt catggtaaaa gcacatgcaa ttttaacggt
    30721 tttgctattt ttgaattcga ttattcccaa tggattttgc cgaattaaaa catcaccggg
    30781 atgacctggc cctcaccctg accctcttcg ttgctttcat tgcgggttcc tggtctgtga
    30841 gcatctccct gccttttgtc ctggctggtc ttttgtttgt tctcttgcgg ttaagaacac
    30901 aggacaagcc cgtccctttt catgcggttg acctggccgt tcttgcagtt ggcttgtatg
    30961 agttgtattc ttatgcggtg tccctttacc gtcccaacag cctgtttggc ctggaaaaca
    31021 tactcttctg gcttgccctg tattttctcc ttcggtacgg attgcggaag tctttggtga
    31081 agactttgtt tgccctagcc ctttcggctt atgcccttat gctggtgctt accgccctgg
    31141 gaaattacat gtccctggat ggttacctgc gggtgcaggg gtgggcagat atttcggagt
    31201 tcaagggttt tctgaattat ttcaaacttt ttctcaacaa ttgggccacg ctttcagttt
    31261 gtctgttacc ctttcccctg atcgcagcca tccatctccg ggccaaccgc atggcctttt
    31321 acggtgccct ttttgttttt tgtatgagcg ttttggcagt cctgatcagc ttttcaagag
    31381 gagcttacct ttctttattt gttttcggca tactctgcac aggggtctta ctgttgattg
    31441 ccaaagatgc cggcagacga ctggtccttc ccgggctttt taccgggggc ctgctgaccc
    31501 tgatcctcct gcttttttac aggccggtat caaatacttt ggccatgaac aaaaccactt
    31561 cgcagcagcg cagcacccat ggccggttga atatcctgga atcgggtctg tgcaagtcaa
    31621 agatttactg gctttccggc accggaacac ataattatcc cctttctccc gatgattgtt
    31681 tccgggaaga ggaagacagc ggatacagcg tgttcaccaa taatctttac ctgcagatcc
    31741 tgatcgaaaa agggctaccg ggctttctga tttacggggc cttgtttttc ctggtccttt
    31801 ggcggttttt tataaatctt ctgcggtcac cggaaaagaa ggaccgctta acctatggtt
    31861 ttctttttgc cggttttgcg gcttatgcgt tccgggaact ttttttctcg agtttttttg
    31921 aaagcagcca ggtgcttgcc ttggtgagtt tgtttgccgc ttatgcggcg gatggcagcc
    31981 ccgtgctttt taccttgcgc aggaattccg ggtattctgt tgcagctggc ttattggtct
    32041 tggtgtccgc ctggtttttt atccaccaac ttaatggacg tggttcggaa aggaaggttg
    32101 aacaagccct ggtggaatgg aaaaacggaa acgcaggaaa ggccgccacc ctgatcggcc
    32161 atgccataaa actaagtcca gttgtgtttc cttatcatga actgcaagga ctggccttgg
    32221 ctcaggacag tctgtccatc cgcaagcttt ttgcaggcaa ccttcaggtg gaccgtgcca
    32281 gcctgtccaa tgccaaatct cattttttac gggctttaca aattaatccc gccgatgccg
    32341 gtgttcattt taacctgggc gtccttgaat ttttacagtc tggcagtctt gcgggtgttc
    32401 cggcctctca ctttatccgc gccctggaac gtgaacccaa taatgttgaa ttcatggtcg
    32461 gattcggaat gcttgccgaa tacagcggca atgccgaact ggcagaccgc ttatacaaaa
    32521 aagcggtacg cttggatccc gaacttatcg attcggagtt tttttccgac ctgaaacacc
    32581 ggaatccctc ttccgactcc ctgctcatcg gtgacgccgt tgccgcctta agatcaaaaa
    32641 tcgattcctc ttataatatg atttttcttg cccgccttgc ccgtttgttg atggcggggg
    32701 aggattctgc gggtattaaa aatatgctgg aagaggtgat ccggaacatc cccacagccc
    32761 accgcccata tttttacctc ggccagttgg cagaaaacgg cgccgattcg tcccgcgctc
    32821 tcgaccttta ccgtaaatct tttttcctgg ggaaagaaga ttatctcgca ccgcttgccc
    32881 tcggcaatta ttattattca aaaatggtgt cttccccgtc gaggagtacg gctctttccg
    32941 ccgcccgtta ttaccgcctc gcgctgatca cctaccttga agcccccagc ctccatcata
    33001 aaagggccaa agcccagtac aaggtcagcg cctccatcgt caatgacctc ctttttaagg
    33061 atctcatgta ttatacccgg gcccgaatgg atttcaggac cattgtaagc cgtatggcgg
    33121 agtcttatga aaagggcggg gatgcaaata tggcggggca ttacaggaaa atggaaggaa
    33181 gggatttgat ggatgtggag atgcggtgag gggcgcacgg agagaaggag ggcacggaga
    33241 tttgaattgt tgggagtatt caataaagtg tgtcagatac ttcagggcgg gctgctgagt
    33301 cttcggccta cggcctcaga ctcagcagcc cgccctggcc aatttttggg atcccatcat
    33361 tggagatgtt tggtataccg gttgccaaaa acttcaataa gttcacgctg gatgggagcc
    33421 cagttaaaca cgtttttttt ccaccctttc gtattcaacc ttaagttcaa atatagctgt
    33481 tttagcaggc tttttggatt gacccaagcc c
//
LOCUS       DAOQXT010000045        32615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1588_length_32615_cov_6.400061, whole genome shotgun sequence.
ACCESSION   DAOQXT010000045 DAOQXT010000000
VERSION     DAOQXT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 32615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32615
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_32615_cov_6.400061"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKM27_09550"
     CDS             complement(<1..352)
                     /locus_tag="PKM27_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07547.1"
                     /translation="MEKLNRKYGLEHGSIPDRVFDYPVRVAQFGTGVLLRGLVDLIFQ
                     QAVDHGLYQGRIAMIKSTSPDTGPFREQDNIYTVVVAGLDAQQNPLVSSHLITCIDRV
                     ISATEEESELKTLFT"
     gene            633..1664
                     /locus_tag="PKM27_09555"
     CDS             633..1664
                     /locus_tag="PKM27_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR07548.1"
                     /translation="MKNKSVTIKEIARQLGISVSTVSRALHDAYDVNQDTRKKVLALA
                     EQLEYKPNPFAISLVKQQTRIIGVLLPEIATHYFSTVVKGIQDVAYHVGYNVMFFISD
                     ESYEREKTIVKNLSANMLDGLLISVSIETTNGDHLKKLTDQGLPLVFFDRYLPDLPVS
                     RVIQDDYKGAYHVTMHLVQQGYQRIAHLAGPKHMEMANRRMQGYIDALRDARIPIRKE
                     YILHSGFTHEDGIRDVSKLLAVRPMPEAIFAVNDRKAIGAMMTLKKSGYLLPDDMGLA
                     GFTNALISEAVEPGLTTIEQSAYQIGTTSCQLLLDRIKTPDQPPQTIVMPGNLIIRDS
                     SKKKIVKIQ"
     gene            1725..3278
                     /locus_tag="PKM27_09560"
     CDS             1725..3278
                     /locus_tag="PKM27_09560"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNR07549.1"
                     /translation="MKYIFSLFLVGFVLLSSHRLQAQGAVAVYVDGGLPTHQISRHIY
                     GHFSEHLGRCIYDGFWVADTLKVAKKDRIRLDIVDALKKIKVPNLRWPGGCFADEYHW
                     RDGIGPRNQRPRMVNTHWGNVVEDNSFGTHEFLELCSLLECEPYIAGNVGSGTVLEMS
                     NWVEYLNHPGGSTLSDLRKQNGHAEPYKVKFWGVGNETWGCGGNMTAEYYADEYKRYA
                     TYVRNYPGAPIHRIASGSNVADYHWTEVLMKNVGPNRMWGTTLHHYSIPSNSWAKKGS
                     ATRFSEQEYFTTLKNAWFMEELVTRHSTIMDKYDLAKRVALVVDEWGVWTDVEPGTNP
                     GFLYQQNSLRDALVAGVTLNIFNNHSDRVRMANLAQTINVLQALVLTRGADLLLTPTY
                     HVFDLYKVHQDAQYLPVRFQSPDYVFENTKIPAVHISASRDSAGLSHISLVNIDPVKN
                     HPVHIRIADIPWSSVTGSIISSAQYTDYNDFDHKNKITIRPFTGASRESGNLVLTLPA
                     KSIVMLELK"
     gene            3310..4737
                     /locus_tag="PKM27_09565"
     CDS             3310..4737
                     /locus_tag="PKM27_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNR07550.1"
                     /translation="MFRLFLLILCIQWFSQCKPKEAMPVADEASRMASADATFKKFCS
                     TCHGDNGDVFVDRQWKHGRTRDSIMMNIRAGFPDLGMPKWDSVITEQEAADLADYLMK
                     AIENRKKFDFQDSVRSSVFVHESMTVKLDTIARGLENPWGMAFLPNGDFLYTDRNGNL
                     YRQSKGVQSMVKGAPQVVAEGQGGLLDVELSPDFANNQTIYLSYSKLKDTAGQKLATT
                     AVQKAKLSGNQITGQKDIFIAMPYQKTRHHYGSRIEFDSTGFMFITVGDRGQHMDSLP
                     QKLDNDMGKVHRIREDGSIPDDNPFYNTPGARKSIWSYGHRNLQGMALQPGTNTIWTH
                     EHGPRGGDELNIPEKGKNYGWPVISYGINYDGTILTPLTHKEGMEQPLVRWVPSIGPS
                     GLTFVKGNKYPAWEGDILMGSLRFRYLERVAIDGKTPGEKENLLKGIGRLRFVEMGPD
                     GFIYVGVEEPGYIFRLLPVDNPSAN"
     gene            4749..5594
                     /locus_tag="PKM27_09570"
     CDS             4749..5594
                     /locus_tag="PKM27_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNR07551.1"
                     /translation="MKKYISAYALLLLIPSALLSQKFSRLMGPRPGIVSYTFRNEFSK
                     DIPGTLDYIQSLGIDNIEFSNLFGQNAHDLKVMIDRRGIRCTSYGVSWDDLMNKRSQV
                     VENAKILGAKYVRVAWMPHDKPWDLEFTRQTAAAFNEIGRDLKAQGLVFCYHNHGYEF
                     QAHGKGTLFDVLVQETNPEYVGYEIDILWVHFPGQDPARLIKKYPRRFKLMHLKDLKT
                     GVQGDLSGKTSTDHDVILGTGQIDLKKVLKASKKSSIEHFYIEDENVKAWDQVPQSLK
                     YLKEM"
     gene            5705..6220
                     /locus_tag="PKM27_09575"
     CDS             5705..6220
                     /locus_tag="PKM27_09575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HNR07552.1"
                     /translation="MIKRRSALTKIASGLAGMISLPAWADSWNPAGLGVFRRLPLSEE
                     ESLAAIVDTIIPATDTPGAKELGVHQLLQKIVADCYDAPAQDTLSMGLVLTDAVSLGD
                     YGSAFRHLNPEQRLAVLKKMAISPHADQKNFLSMVKRLTIDGYMRSEYVMTNITKFEF
                     APNRFYGCRPV"
     gene            6259..7971
                     /locus_tag="PKM27_09580"
     CDS             6259..7971
                     /locus_tag="PKM27_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNR07553.1"
                     /translation="MSNLNINVKKKTTYDAIVIGSGISGGWSAKELTDRGLKTLLLER
                     GRDVKHIVDYPTATLQPWEFEHRNQLPLEVKKNNPIISKCYAFYEGTTHFFVKDAEHP
                     YIQDKPFDWIRGYQVGGKSLMWARMTQRWSDHDFTGPARDGYAVDWPIRYEDLAPWYS
                     HVEKFAGITGNRDGLDTLPDGEFLPAYELSCVEKYFKEQMAKHFPGRHVISGRTAHLS
                     EPQAIHYKQGRGKCVNRTICERGCPYGGYFSSNASTIPWALKSGKLTLRPHSVVHSII
                     YDEQKGKATGVRVIDANTKEMMEFYAKIIFVNAAALNTNLILLNSTSNRFPNGLGNDS
                     GVLGKYVAFHNYRARISAEYDGYLESRTEGRKPSSGYIPRFRNVYKQETDFLRGYASG
                     FSASRGEVRSNEGLGTELKNNLHTAKWSSWRVGSGIMGETIPKESNQVTLDKDKKDDW
                     GIPLLHIKVDYDDNDEKMVRDFLEQFSEMYAKAGFTNIRTSDSKQAAGLDIHEMGGVR
                     MGHDPKTSMLNKWNQLHLCRNVFVTDGACMTSTSTQNPSLTYMAFSARAAQYAVKELK
                     KRNL"
     gene            8011..8466
                     /locus_tag="PKM27_09585"
     CDS             8011..8466
                     /locus_tag="PKM27_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07554.1"
                     /translation="MEQDTPTELLNHDSKNHLATAGTWARLLGILGFIFTAFIFLAAL
                     MMLLGGSYLNQFLAESSGEEFPGMARGLGTFTGLLYLVIGGIYFLISWWTFRFGNEIR
                     EGLMTHSPEHMAAAFNNLKNYFQAYGVLTLVAVGFMLLSVLGVMLVGFF"
     gene            8556..9239
                     /locus_tag="PKM27_09590"
     CDS             8556..9239
                     /locus_tag="PKM27_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07555.1"
                     /translation="MQAKEWLIKGKSYLSEVPLESVDSKISQRLHVSMIRGRLQLTTD
                     HVIYSWEDLYRNFRYASNRINWKKFKPKNVLVLGLGLGSVIQILERKVTYPLSITAVE
                     LDEGVLYLVKKYSASKFRSPIQYLCADAYDFVQQNRQKFDLILFDIFVDDYIPSQFES
                     LAFTKKLASGLTPGGLILYNRIAMEPSQIMQNMEFYDQAFSKVFPDAEILQIKTNWVF
                     ASNKHLLKS"
     gene            9275..9865
                     /locus_tag="PKM27_09595"
     CDS             9275..9865
                     /locus_tag="PKM27_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07556.1"
                     /translation="MFTINIYLKFALIALCLFGGVALAFAYGFWWAFPVILIGILLLI
                     SYIFLGTVQSAAQLMEKMDFAGSERRLGLTFFPQWLYRPNRAYFYMIKGSIAAQLKDY
                     DRAEQLLLRSKSIGLPSANEKAMVALQLANFSAVKNKWNQAQIYMKEIKEYKVTEPMI
                     KDQIKQFEKAMQQRGQQKHLMGGDPSMMRMRRPRKM"
     gene            complement(9941..10594)
                     /locus_tag="PKM27_09600"
     CDS             complement(9941..10594)
                     /locus_tag="PKM27_09600"
                     /inference="COORDINATES: protein motif:HMM:NF039641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HNR07557.1"
                     /translation="MNLPFTIDEFLEVFRKYNLSVFPMQVVFYLFGGLAIFHSIYKTR
                     ISDKINLLFLSLLWLWSGIVYHILHFSGINKAAHLFGALNIVQAGLLYFYGIHREKIT
                     FQFKWDIYGVAGGLLILYALLVYPILGYLLGHQYPASPTFGVPCPTTIFTFGMLLWSG
                     NKVPMAVLIIPFLWSLIGTTAAIYLGMTEDLGLFLSALITAFLLYFRSPLSPKTMVG"
     gene            complement(10703..11428)
                     /locus_tag="PKM27_09605"
     CDS             complement(10703..11428)
                     /locus_tag="PKM27_09605"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNR07558.1"
                     /translation="MDRKKFIKTSTILSAAALLPSDSVFAHNLAENGIDRLVDAQGNF
                     MLQPLPYAKNYLEPYMDEETLHLHYTFHHGGAVNGANKDLQMVRKALDENAVETVDYW
                     TKKLAYHLSSHVLHTIFWTNLSNKKSAPAGDLLKRIERDFGSYDKLKLLLSKTSKDVD
                     GNGWGILGYQPYTDKLTVLQCENHEKLTQWGVIPLLVIDVWEHSYYLKYRNRRAEFVD
                     NLYQIIHWDNVAQRLDVAVKLMK"
     gene            complement(11432..12643)
                     /gene="chrA"
                     /locus_tag="PKM27_09610"
     CDS             complement(11432..12643)
                     /gene="chrA"
                     /locus_tag="PKM27_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNR07559.1"
                     /translation="MEPMDKPADIRQRPAQIFRRFLRFGCLAWGGPVAQIAMLRQELV
                     EEEKWVSSERFNRALAVYQVLPGPEAHELCVWFGMIAGGRTGGFLAGLGFMLPGFLLM
                     LALTWFYLSFGIGSPVLQAVFTGFQSAVIALIFSAVHRIGKHILVKGSLITLALLSAL
                     ATFCGTGFIPVLAVAGITNALWMTGRRIAGAVIAILLTAYCTWSFSPGMFSNLSPATT
                     TLEVNLESGSSLGSVFLTGLKGGLLTFGGAYTVIPFIQQDAVICHGWMSNEQFLDGIA
                     LSGMLPAPLIIFSTFVGYFGAGWSGAFIITLAIFLPAFCFTLIGHRVIESVIENNSLH
                     HFLDGIAAGVTGLIAVTAIQLFQPTINGWSNFIIFIAALFVVYQVKTKFTAAFVILGA
                     GVISLLYYLIF"
     gene            complement(12656..13108)
                     /locus_tag="PKM27_09615"
     CDS             complement(12656..13108)
                     /locus_tag="PKM27_09615"
                     /inference="COORDINATES: protein motif:HMM:NF021357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNR07560.1"
                     /translation="MKWITRERPKIDRIACPWLIKNFVDPDAEFIYVPKPFVFDRAKE
                     IGAIPYDIPGAEYSHEGDLCTFDFIIRKHGLTDPALLQLALIVRGADTDRFHLAPQAA
                     GLWAISAGLSYNNQNDQDMLAIGMIIYDALYSWAKHLQGEKHTWNPQL"
     gene            complement(13166..14029)
                     /locus_tag="PKM27_09620"
     CDS             complement(13166..14029)
                     /locus_tag="PKM27_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNR07561.1"
                     /translation="MNWITRERPKIDRIACPWLIKNFVDKEAQFFYAPAGEVQQKARL
                     LDATPFDIPGVEYSHEGEFCTFDKILKKHELKDPSLLQLAVIVRGADTDRFHLAPQAA
                     GLWAISAGLAGRIRDDQKLLEFGMVLYDALYYWVKHLQHEKHTGDPVEQLLIQMLNTY
                     LSGKVAPGKRIPAWAKGLKETIQDYVDTNFSLSLKKISKDLDLHPAYVSRTFSKYFDN
                     LSFGEYIRKQRIEKAIHLIKTSDYSFTEIAYLTGFSDQSHFNRIFKKQTGIQPSVYKK
                     SLKKGKPDTSS"
     gene            14113..15999
                     /locus_tag="PKM27_09625"
     CDS             14113..15999
                     /locus_tag="PKM27_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNR07562.1"
                     /translation="MKRLHFLILFLLAVLPHAHSQTGEEVYKKLQAIAVIDQKVMMPM
                     RDGIRLATDIYRPKGDQKVPVIFSRTPYNFNSWGNGEQRFNSYSAAYEAVRRGFAYVV
                     QNERGRYFSEGQWDILGTPVTDGSDAFSWLEAQAWCNGKIGLTGCSSTAEWQMAVAAQ
                     DHPALATAIAQGFGAGVGRIGNYYEQGNWYRGGATQMLFISWLYDTQHDIFRPSIPKN
                     ASQEDLVRLQRFYDLDPEYPRVDWSKAFQHLPVRDIIRNVNGPKSIFESMVARKPNDP
                     AWYQGGLYHDHMPYEKPTFWFVSWYDVSTSPNLAMVNRIKSQAKKAGVADQQYLVIAP
                     TLHCAYKRASENTLVGERSVGDARLDYDSMTYDWFEYWLKGENNGVLTRIPKVQYYTM
                     GSNKWQSSETWPPGSSSMTTWYLHSQGKANSVFGDGRLSGQKPGPSLPADGFVYDPAF
                     PVPSYGGNVCCMGNALAGGSFDQRQMETRNDILVYTTDPLQQGLEISGFIEATLFVSS
                     DAKDTDITIKLLDVYPDGKAYNLDETIQRLRYREGYDKEVFMEKDKVYQVEMTPMSTS
                     NYFAPGHRIRIEISSSNFPRFDRNLNTGGNNYDESKGVVANNRIHHNAVYPSQVRLPV
                     VKSQ"
     gene            complement(16188..17480)
                     /locus_tag="PKM27_09630"
     CDS             complement(16188..17480)
                     /locus_tag="PKM27_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR07563.1"
                     /translation="MNRKEFIHKSSLGALALTASPFLIRKQAAKYTTVLIGSGWWGTN
                     ILREAIRSGEVRVAALCDVDESQLAKCKEEVARLCNDQPKIYKDFRECIAKEKPQIVI
                     NATPDHWHALVAIEALNQGAHVYLEKPISHTVLEGAAIEKAARDNKRICITGFHRRYS
                     PHNASGIEFLRSGKVGKIREVKAFVEYGGGDRVQREEQSPVPAGLDWDMYCGPAKLVT
                     YNKGMHPRGWRQYRNFANGQLGDWGPHWFDQILWWSDEKAPKKVYSVATQRDRDSWSD
                     APTSQTVVYSFESFHLTWNHNLFNPHRETKTENVGVYFYGTEGVFHMGWIKGWTFFPN
                     DEKKETLHEEAKLNQPDLQNIDLVWQDFLKSIKSGQLPHADIAHGRRATNMALLGMLS
                     ADLGRSIEWDDQNNRILKDKAANALLKRQYRSPWKYPL"
     gene            17609..19303
                     /locus_tag="PKM27_09635"
     CDS             17609..19303
                     /locus_tag="PKM27_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07564.1"
                     /translation="MERRQFLRNAAIGMAGTMALNGWSCRPDQAWLDQLIKANDHRIP
                     GLLGNQVQDALSPLLGSVPDPFQIHHPGNAAGLVQAFTCAYVSPGSTYHGDEKLLAAM
                     SLAMIYLRRVQHEDGTIDLLTTNFHSTPDTAFVVEPLALSYKLLSRESDGKSKELQNQ
                     IRDFLVKAGLALSTGGIHTPNHRWVVCMALARLSELFPDPAYSNRADAWLTEGIDIDS
                     DGQYTERSTSVYSPLTNRCLITIARLLNKEYLFEPVRKNLEMTLYYLHPNGEIVTETS
                     RRQDQYQARNASAYHYPYLFMSLKDQNGAFGAMARKIEKENSPGELTGSLAYYLEDAQ
                     LLSTYPTGALPAEYEKLFDESGLVRWRNWDMDASLLSKNSTLFTLSNKDALLQAVRVA
                     TAFFGKGQFIAEKLEKTGNGYRWTQTLEGPYFQPFPADSLPGDGDWEKMPRDQRPRSE
                     VQRMEYILEAHHEGDTWSLDFDLHGTDRVPVALELSFRSGGKLTGVEPVPGIEHAFFL
                     KSTNGKYTMKGSNLFFGPGKNEHAWTQLRGAEPKIPGQSVYITGYTPFKYQLKIHD"
     gene            19462..20481
                     /locus_tag="PKM27_09640"
     CDS             19462..20481
                     /locus_tag="PKM27_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNR07565.1"
                     /translation="MAKAQQTYQDQTFQLKKYEAPKSKQEWLKRQKFIRKTVWDCFGD
                     LPPRPQKPEVKIKSREEKDGYILESFEFFNGVDATVPGIVLIPKGLTGKAPAVLYQHY
                     HGGEYYHGKDELFSSKLIPFGPGPELVKAGYVVMAIDAYAFGERSGKGPNGSKEKGSG
                     EELTWAKMNIWKGRNHWGMMVRDDLISLDYLAGRAEVDPKRIAAIGLSMGCFRAFYLA
                     ALDERVKVTVPVSCITRNADLIKGGALNLHGVYYYVHGLMKHFDTESIMGCIAPRTLL
                     NLSGADDMLEPLEGQKFINEALGGIYKVVGSPDQFKYKIYEGVGHAYTPEMWKETLEW
                     LKEKL"
     gene            complement(20476..21636)
                     /locus_tag="PKM27_09645"
     CDS             complement(20476..21636)
                     /locus_tag="PKM27_09645"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNR07566.1"
                     /translation="MSRIKVLGCGMVGSAIARIMAAKHEVSVLDISRRSLDSLPHDIK
                     KIKADIGDRIALQKNIKNADLVIGAVPGFMGFEVMKNTIEAGKNMVDISFFPENAFKL
                     NALARQNNVIVVFDCGVAPGMDNILLGYHDQRMRVTGFACYVGGLPKKRQVPFEYKAP
                     FSPADVLEEYTRPARLKEKGKVVVKPALTDLEYIHTGKGLKLEAFNSDGLRSLLTTMK
                     HIPEMKEKTLRYPGHAGQMALLREIGLLDKNPVKVKNKEVIPLELTSTLLFKHWKYLP
                     GEADFTFMRTLIDGVEEGRKIRYTYNLYDEYDPATSMSSMARTTGLTACAAAELILAK
                     KITKPGVMAPETLGRSSVCFEFILNYLKDHRIYYRKRKSIFTPPTTVHFPPS"
     gene            21906..23186
                     /locus_tag="PKM27_09650"
     CDS             21906..23186
                     /locus_tag="PKM27_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNR07567.1"
                     /translation="MINPSGRNHNNADSAFQEIEQWMKNLAENGGNYVRIWLSQSFWD
                     MEDQAAGKYNEEKVHRIDRFVAMARKYGLRIKMTLEHFRSITMEENPQSWAVKGVYHK
                     SKGGPLDSIRQYLSSPEGRRLFLNKIDFYQKHFGNDTLFFGWELWNEMNAVKGPEDDL
                     FFEWNETMLREVKQRFPENLVMQSLGSFDHENVRPVYKRMMLLPGNEVAQVHRYLDLG
                     AKMEICQAPMDLIASSAVDELVTYQTGKPLILAETGAVEPRHTGPSKYYPRDTAGTLL
                     HDILFAPFFSGSAGAGMAWHWESYVHKNNLWYHFGRFQEAIKGIDPIEEGFVPVRTET
                     HAVRIYHLKGKKTSLFWIRDKSSHWESELEKGILPQSRKGIRLTPNETGLPRRARTIE
                     VYDPWKNQWSVVKKKGTGFTLPDFKRSLVVRVVH"
     gene            complement(23311..23853)
                     /locus_tag="PKM27_09655"
     CDS             complement(23311..23853)
                     /locus_tag="PKM27_09655"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNR07568.1"
                     /translation="MNELPLNKAFMLLEPGPVVLVTTADKGSKNIMTISWHMVMDFTP
                     RFALLTGPWNYSYKALVKTKECVLAIPALDLSKKVIDIGMCSGADTDKFKKFRLTALE
                     AKQVDAPLIRECLANIECRVVHHLKKHKLFILEAVQAWIEPNRKERRTIHSRGDGFFI
                     VDGRTLNYRRRMFEKLPPGV"
     gene            complement(24008..25249)
                     /locus_tag="PKM27_09660"
     CDS             complement(24008..25249)
                     /locus_tag="PKM27_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR07569.1"
                     /translation="MKITILTLGTRGDVQPFAVLGQALTKRGHEVTLSTGKNFESLVR
                     SYGLGFVPVEADFQAMIHSDAGKQMKKNPLRARKEIGKVVFPMLANAFVTFYELSKRS
                     DLVLFHVKTMAENFSDQFPGKMIMANVVPASQATREFPNPVFSAMPLPRFLNKFSYKL
                     TEWGLAMWKKAIDDFRVKAGLPVHFIKPSLPSLYGISPQFLKKPDDYPANAHFTGFWS
                     APSDEALSPEILHFIQAGEAPVLITFGSMPFESRLDLPEALRSARETLGIRLLVIKGW
                     GITSSADQNSDTDILFVDSAPYDKLIPLVRAVVHHGGIGTMAACLQAGKPFMTCPVLY
                     PLGDQHFWGTLAFNKGVALKPLPLKKLTQDLLNDGIRELISNPEIHARALRLAEEIKK
                     EDGIKKAIEIIENPDLREALR"
     gene            25299..26237
                     /gene="queG"
                     /locus_tag="PKM27_09665"
     CDS             25299..26237
                     /gene="queG"
                     /locus_tag="PKM27_09665"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNR07570.1"
                     /translation="MNPAEKSAWIKSEALKSGFLTAGIARAEPLNEEQKQLEAWLRKG
                     YQGDMSYMERNLDKRVDPAGLVPGAKSVISLAYNYYTPDRPVPGSLKISTYAYGRDYH
                     KVLKKKLKALWNSIRMQLSPDAQARYFVDSAPVMERQWAARAGLGWLGKNTLLIHPGH
                     GSFFFLAEIITDLELEYDEPTWDHCGTCTRCIDACPTQAIAPAGYELDARRCISYLTI
                     ETQNDIPEEQVQHMEGYIFGCDICQQVCPWNRFSRPHEEPEFEPGNDFLNWNRDQWIK
                     LDESQFAETFGHSPVRRAGYDKLKRTIGSVMKGGKG"
     assembly_gap    26586..26685
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26826..27848
                     /gene="wecB"
                     /locus_tag="PKM27_09670"
     CDS             26826..27848
                     /gene="wecB"
                     /locus_tag="PKM27_09670"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNR07571.1"
                     /translation="MILVCYGTRPEIIKMSPVIEELKKQELRFKTVFTGQHKDMYEQF
                     KHTIRTPDYWLDVMRSKSSLGDTIGSIMHKLDLLLGKGGIDLVLVQGDSSSSFACALS
                     AFHHKVPIGHVEAGLRTFNIQSPFPEEGYRQMITRIVNLHWAPTQKALDNLLREGVQN
                     ARLTGNTIIDACLGTQNEIRYGNKVLVVLNRRDNYGERMKKLFTEVEELANRHPELEI
                     IFPMPHNPNVKSHRYIFKKVKVIEPLEYSEILKLISEAKFVISDSGGLQEECAAFRKK
                     ILVCRENTERPEGLEAGIGKLVNTEVLQHYRWAMDQPEWTGENPFGDGNARIRIVEHI
                     REFLGI"
     gene            28026..29144
                     /locus_tag="PKM27_09675"
     CDS             28026..29144
                     /locus_tag="PKM27_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNR07572.1"
                     /translation="MNINSKFFPFALLFSAIALFWTCKQEEEGPIKTLITGEITGYPD
                     GTLTIYRVVDEDSSRVDTFPLRDGKFEISLSDHEPRMANLVFPDGMTAVQFFTEAGTI
                     TMKGTREEAANLEVSGTPANELNKEMKAIYAGFEQRYNDLMEQATKAQQTEDQAALDT
                     LEPLLMKFEGEVSRSHVDFAMKHPNTILSAYLGLSASMSPGLDLKPLYESLSDEVKNT
                     FFGLRLKNVVESAAKTAIGSAAPDFEGISPDGKILRLSSLKGKHLLVDFWASWCMPCR
                     QENPNVVRVYDNYKNKGFEILGVSLDQEKEKWVDAIAQDGLAWPQVSDLLGWKSKIAA
                     MYGVQGIPQNFLLDHEGRIIAKGLRGQDLENKLKELLN"
     gene            29173..29841
                     /locus_tag="PKM27_09680"
     CDS             29173..29841
                     /locus_tag="PKM27_09680"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HNR07573.1"
                     /translation="MFVSITDYLYEFKANWAVGLQDKKFFYTLAGIFAALIPLGLYLV
                     PYLQFIEHRQGVILNDFLLDQFPRADYSNLIFSIIYTSACFMILYSLRSPWMLLRNLL
                     IFIVLQYIRNLCLYFVPLAPPEGIVPLSDPVLEMVAYNRQPLLQDLFFSGHTATCVVF
                     ALLTRNHTGLFSTFIVITVVMICLLMIQHCHYTIDILGGIVFAVLAYRLVDRSWLTMK
                     LPFR"
     gene            29874..31061
                     /locus_tag="PKM27_09685"
     CDS             29874..31061
                     /locus_tag="PKM27_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNR07574.1"
                     /translation="MYSLDLKIKTLADQVDYLQNHNIYLYYREMDSAQDAEMMADGRK
                     ILMFGSNSYLGLTNHPKVKEAAIQAIREYGTGVSGSRLLNGNLRIHRELEERLAAFVG
                     KEDAVVVSTGFQANLAAIPPIIDRSGYLIMDKLSHASILESAALSFGRKSKFEHDDLN
                     SLEDKLSICPADATKMVVTEGVFSMDGDIPDLPGLTALANRYGALVMVDDAHALGVLG
                     QNGSGSSSHFQVTDRVDLITGTFSKSLAALGGFVASSFEIIKLIKHTSRSQMFSAGLQ
                     PASVAAVMAALEVIAEEPERIEQLWKNTRYAMELMKQTGFDNGTAETPIIPVYVRDDD
                     KTYLLARRLYDDGIFVNPVVSPATSPDQALIRFSIMATHTRSQIEEAIDKMHFHAKAL
                     EIL"
     gene            31070..32212
                     /locus_tag="PKM27_09690"
     CDS             31070..32212
                     /locus_tag="PKM27_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07575.1"
                     /translation="MIEIKLVRNKRELKRFIDFSYDLYSGDSLFVPELFIAQRDYLDP
                     SKNPFFEHARLELFLAEKGGKVAGRIGVVRDEVLIEYTGEKTGVFGFFETIEDEEVAT
                     ALLDAAAAWCRSQGLICLEGPYNFSTNYPSGLLIDGFQYSPAVMMTYNKRYYQGFLEN
                     YGFTKKTDVLAYHLDADLFPDRLEKNLGMLERRLAEKGITVRKIDMKHFYRDVKSAIN
                     VYNQAWSKNLGFAPFTEKEFMHVARDLKLILDPELVLLAEREGRVIGFSLSVPDINEV
                     QKGIRRGRLLPFGIFKLLFNKSKIKNVRILALGVVEEFRKLGLDAYFYARAFSYVRRH
                     KRLQGGEAGWVLENNAEMNQALVKMGGKVRKKYRFYRLNLGNHKNP"
     gene            32209..>32615
                     /locus_tag="PKM27_09695"
     CDS             32209..>32615
                     /locus_tag="PKM27_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07576.1"
                     /translation="MRLSGIEFDRDATILLLSAPVLLSIYYYHGSAESFFRYFNWAPE
                     GVGPVYWRFFVFFLLLGIIPLSIIFWVFRDKPYSFGLALGDWRKGLSLALILIPLLIL
                     PLAWGASQVPEVQTEYPLAKVLFSQGPLFWQYSL"
BASE COUNT         8340 a         8070 c         8268 g         7837 t
ORIGIN      
        1 tggtaaacag ggttttaagc tcggattcct cttcggtggc gctgatcacg cggtctatac
       61 aggtgatcaa atggcttgag accaaagggt tttgctgggc atcaagtccg gcgaccacga
      121 ccgtataaat attgtcctgt tcacgaaaag gtcctgtatc cggagaggtg gatttgatca
      181 tggctatgcg accctggtac aggccgtggt ccaccgcctg ttgaaagatc aggtccacca
      241 ggccccgcag cagcacaccg gtcccaaact gggccacccg aaccgggtaa tcgaagaccc
      301 ggtcaggaat agacccatgc tcaagaccat attttctatt cagtttttcc attcaatatt
      361 ccgggtcggt tggaataaaa gcgataaaat taaggggagg aattaaggag gggattaaat
      421 ttatattggg aacaaagtaa aacggggagg atgttggcaa cggggtcagt agggcatgtt
      481 ccggtatccc ttgcaggcag tcaaagatgg ttttttttat acatttatct ggatgttttg
      541 ccaaaatcaa aaagcttata ccgcctaaac aaatttaact tattcctgca cgggaccgga
      601 tccaaattgc ggtaacgtta ccgaatcaaa tcatgaaaaa caaaagcgtc accattaaag
      661 aaatcgcgcg ccagttgggg atatctgtat ccaccgtatc cagggccctt catgatgcct
      721 atgacgtgaa ccaggacacc cgcaaaaagg tactggccct tgctgaacag ctggagtaca
      781 aacccaatcc cttcgccatc agcctggtca aacagcaaac ccgcatcatc ggggtattgt
      841 tgccggagat cgccacgcat tatttttcta cggtggtgaa agggatacag gatgtggcct
      901 atcatgtggg atacaatgtg atgtttttta tatcggatga atcctacgaa agggaaaaaa
      961 ccattgtcaa gaatttgtcc gccaatatgc tggacggcct gctcatttcc gtatctatag
     1021 aaaccacgaa cggcgaccat cttaaaaaac tgaccgacca gggcctgcct ctcgtctttt
     1081 ttgaccggta ccttccggat ctgccggttt cccgggtgat ccaggatgat tacaagggcg
     1141 cttaccatgt cactatgcac ctggtgcagc aagggtacca gcgcattgcc cacctggccg
     1201 ggccgaaaca catggaaatg gccaaccgcc ggatgcaggg ttatatcgat gccctccgcg
     1261 atgcgcggat tcccatccgg aaagaataca tcctgcattc aggctttacc catgaagacg
     1321 ggatccggga tgtgtccaaa ttactggcgg tccggcccat gcccgaagcc atatttgccg
     1381 taaacgaccg gaaagcgatc ggggccatga tgacccttaa aaaaagtggt tacctgcttc
     1441 ccgacgatat gggcctggcc ggttttacca atgccctgat cagcgaagcg gtggaacccg
     1501 ggctcaccac cattgaacaa tcggcctacc agatcgggac gaccagttgc cagcttttgc
     1561 tcgaccggat taaaaccccg gatcagcctc cccaaaccat tgtaatgccc ggaaacctca
     1621 tcatccggga ttccagcaag aaaaaaatcg tgaaaataca atgaggagaa ccccttgata
     1681 ttcaggcggc tttatatatt gcattttaaa taaaaatttg gggcatgaaa tacattttta
     1741 gtctattcct ggttggtttt gtattgctca gcagccatcg gttgcaggca cagggcgcag
     1801 tagccgtcta cgttgatggg gggcttccaa cccatcagat cagcagacat atttatgggc
     1861 atttttcgga acacctgggc cgttgcattt atgacggatt ctgggttgcc gacaccctca
     1921 aagtggccaa aaaagaccgg atccggctgg atatcgtgga tgcccttaaa aagataaagg
     1981 ttcccaacct tcggtggccc ggcggatgtt tcgccgatga atatcactgg cgggatggga
     2041 taggcccaag gaatcagagg ccccggatgg tcaataccca ttggggaaat gtcgtggaag
     2101 acaacagttt cggaacacac gaattcctgg aattatgttc cctgctcgaa tgtgagccct
     2161 atatcgccgg aaacgtcggc agcggaacgg tcctcgaaat gtccaactgg gtggaatacc
     2221 tgaaccatcc ggggggaagc acgctttcgg atttaaggaa acaaaatggc catgccgaac
     2281 cgtataaggt aaaattctgg ggggtgggca atgaaacctg gggatgcgga ggcaatatga
     2341 ccgccgaata ttacgcggac gagtataaac ggtatgccac ttacgtccgg aactatcccg
     2401 gggcgcccat tcacaggatc gccagcgggt ccaacgtcgc ggactaccat tggaccgaag
     2461 tcctgatgaa aaacgtaggc cccaaccgga tgtggggcac cacgcttcac cattattcca
     2521 ttcccagcaa ttcctgggca aagaaaggtt cggccacccg gttttcggaa caggagtatt
     2581 ttaccaccct taaaaatgcc tggttcatgg aagagctggt cacccggcat tccaccatca
     2641 tggacaaata cgatctggcc aagcgcgtcg ccctggtcgt cgatgaatgg ggcgtgtgga
     2701 cggacgttga accggggacc aatcccggtt tcttatatca gcaaaacagc ctccgggatg
     2761 ccctggtggc cggggttacg ctcaatattt ttaataacca cagcgaccgg gttcgcatgg
     2821 ccaacctggc ccagaccatt aatgtgcttc aggccctggt gctcacccgg ggggcagacc
     2881 tcttgctgac gccgacctat cacgtctttg acctctataa agtccaccag gacgcccaat
     2941 acctgccggt tcgtttccag tccccggatt atgtatttga aaacaccaaa ataccggcgg
     3001 tccatatttc agcctccagg gattccgcgg gcctttccca catttccctg gtcaacatcg
     3061 atccggtgaa aaaccatccg gtccatatcc ggattgcgga tattccctgg tcttccgtga
     3121 cgggttcgat catcagttcc gcccagtaca ccgattacaa tgattttgat cacaaaaaca
     3181 agatcaccat ccggcccttt accggggcat cgagggaaag tggaaacctg gttttaactt
     3241 tgccggccaa aagcatcgtc atgcttgaat taaaataaag gttaatacaa ataaattcaa
     3301 tttaaaatca tgttccgcct cttcctctta atactctgta ttcaatggtt cagccaatgc
     3361 aagcccaaag aagccatgcc ggttgcagac gaagccagcc gtatggccag cgccgacgcc
     3421 acttttaaaa aattctgttc tacctgccac ggggacaacg gggatgtatt tgtggaccgg
     3481 caatggaaac acggaagaac ccgcgattcc atcatgatga atatccgcgc gggtttcccg
     3541 gacctgggta tgccgaaatg ggattcggtg atcaccgaac aggaagcggc ggacctggcc
     3601 gattacctga tgaaggccat tgaaaacagg aaaaaatttg atttccagga cagtgtccgg
     3661 tccagtgtct ttgtgcatga aagcatgacc gtaaaactgg atacgattgc acgcggactg
     3721 gaaaatccct ggggcatggc ctttttaccc aatggagact tcctctacac cgaccggaat
     3781 ggcaatttgt accgccagtc caagggcgtt caaagcatgg tcaaaggggc tccgcaggtg
     3841 gtcgccgaag gacagggtgg ccttctggac gtggaattgt ccccggattt tgccaacaat
     3901 caaaccattt acctgtccta ttccaaactc aaggataccg caggccagaa attagcaacc
     3961 accgccgtgc agaaggccaa actcagcggc aatcagatca ccggtcagaa ggatattttt
     4021 atcgcaatgc cctatcaaaa aaccaggcac cattatggct cccggatcga attcgattcc
     4081 accggcttca tgtttatcac cgtaggcgac cggggccagc atatggattc ccttcctcag
     4141 aaactggaca acgatatggg caaggtgcac cgcatccggg aagacgggag catcccggac
     4201 gacaatcctt tctataatac tcccggggcc cgcaaatcca tctggtcata cggtcaccgg
     4261 aatctccagg gtatggccct gcagcccggc accaatacca tctggaccca tgaacacgga
     4321 ccaaggggag gggacgaact gaatatcccg gaaaaaggta aaaactacgg atggccggtg
     4381 atcagctatg gtattaacta cgacggcacc atcctcaccc ccctgaccca taaggaagga
     4441 atggaacaac ccctcgtccg gtgggtgccc tccatcggcc ccagcggcct gaccttcgta
     4501 aaaggaaaca aatacccggc atgggaaggg gatatcctga tgggttcttt gcgattccgg
     4561 tatctcgagc gcgttgccat agacggcaag acaccggggg agaaagaaaa cctgcttaaa
     4621 ggcattggcc ggctgcgttt tgtcgaaatg ggtccggacg gatttatcta tgtgggtgtg
     4681 gaggagccgg gatatatctt cagattgcta ccggtggaca acccatccgc caattaaaaa
     4741 ccagtcctgt gaaaaaatat atttccgcgt atgcattgct gctcctgatt ccatctgccc
     4801 ttttatctca aaaattcagc aggctcatgg ggcccaggcc gggtatcgtg tcctatacct
     4861 ttcgcaatga attcagcaag gatatcccgg ggaccctgga ttacattcaa tcgctgggca
     4921 tcgacaatat tgaattttcc aacctcttcg gccaaaacgc tcacgaccta aaggtcatga
     4981 tcgaccgccg gggaatccga tgcacgagtt atggggtatc ctgggacgac ctgatgaaca
     5041 agaggtccca ggtggtcgaa aatgcgaaga tcctgggcgc caaatatgtg cgtgtcgcct
     5101 ggatgcctca cgacaagccc tgggacctgg aatttacccg gcagacggcg gccgccttca
     5161 atgaaatcgg cagggacctt aaagcccagg gtctcgtctt ttgttaccac aaccatggtt
     5221 atgaattcca ggcccatggc aaagggactt tgtttgatgt gctggtacag gaaaccaatc
     5281 cggaatacgt cgggtatgaa atagacattc tttgggtcca tttccccggg caggatcccg
     5341 ccaggcttat taaaaagtac ccccggcgct ttaagctgat gcacctcaaa gaccttaaga
     5401 ccggagtaca gggagatctt tccggaaaga cttccaccga ccatgatgtc atcctgggaa
     5461 ccggacagat cgatcttaaa aaagtgctca aagcatccaa aaaatcttcc attgagcatt
     5521 tttatatcga ggatgaaaat gtgaaagcct gggaccaggt gccgcaaagc ctgaaatacc
     5581 tgaaagaaat gtaattcgaa ggattcgatc taccatcatt cttggataaa tccgtcctat
     5641 tcttccggag tttcatatcc ggagatgtcc gtaattttaa acctccatct ttaagataaa
     5701 aaatatgatc aaacgcagat cggcattgac aaaaatcgct tcgggccttg ctggcatgat
     5761 cagtcttcca gcctgggcgg attcctggaa cccggcggga ctcggggtat tccggcgact
     5821 gccgctttcc gaagaggaat ccctcgcagc catcgtcgat accataattc ctgctaccga
     5881 taccccgggt gcaaaagaac tgggtgttca tcaattgctg cagaaaatcg tggcggattg
     5941 ttacgatgct ccggcccagg acaccctcag catgggcctg gtgctcaccg atgcggtatc
     6001 cctgggagat tatggaagtg cttttcgcca tctgaatccc gagcaaaggc ttgccgtcct
     6061 taaaaaaatg gcgatttcgc cccatgcaga ccagaaaaac tttttatcca tggtcaaacg
     6121 gctgaccatt gatgggtata tgaggtcgga atatgtgatg accaatatca ccaagtttga
     6181 gttcgctccc aacaggttct acggttgccg tccggtttaa tgtcctttat tatttctatc
     6241 tgatttcaaa ttaaaatcat gtccaacctc aatattaacg taaagaaaaa aactacttac
     6301 gatgccattg tgataggttc cgggatcagc ggcggatggt cggccaagga actgacggac
     6361 cgtggcctta aaaccctgct gctggaaaga ggccgcgatg tgaaacatat tgtggattat
     6421 cccacggcga ccctgcagcc ctgggaattt gagcaccgca accaattgcc cctggaggtg
     6481 aaaaaaaaca atcccatcat atccaagtgt tatgcttttt atgaaggaac gacccatttt
     6541 tttgtaaaag atgcggaaca cccctatatc caggacaagc cctttgactg gatccggggt
     6601 taccaggtgg gcggcaagtc cctgatgtgg gcacgcatga cccagcgctg gagcgatcat
     6661 gactttacag ggcctgcccg cgatggttat gccgtagatt ggcccatccg gtacgaggac
     6721 ctggcgccct ggtacagcca tgtggaaaag tttgcaggca tcacgggcaa ccgggacggc
     6781 ctggatacgc ttcctgacgg ggagttttta ccggcttatg aattgtcctg cgtggaaaaa
     6841 tatttcaagg agcaaatggc caaacatttt cctggccggc atgtgatcag cggccgcacc
     6901 gcccacctct ccgaacctca ggccattcat tacaagcagg gcaggggcaa atgcgtcaac
     6961 cggaccatct gtgaaagggg ttgtccttat gggggatatt tcagcagcaa tgcctccacg
     7021 atcccctggg cgctgaaatc cggcaagttg acgctacgcc ctcattccgt ggtccattcc
     7081 atcatatacg atgaacaaaa aggcaaagcc accggagtcc gggtgattga tgccaacacc
     7141 aaggaaatga tggaatttta cgccaaaatc atttttgtca acgcggccgc cctcaatacc
     7201 aatctcatcc tcttaaattc cacctccaac cgtttcccca acggcctggg caacgacagc
     7261 ggggtattgg gtaaatatgt ggccttccac aattaccggg cgaggatctc ggccgaatac
     7321 gacggatacc tggaaagcag gacggaggga aggaagccca gttccgggta tattcccagg
     7381 ttcaggaatg tgtataagca ggagacggat tttctgaggg gttatgcctc cggcttcagc
     7441 gccagccgcg gtgaagtccg gagcaacgag ggcctgggca cagagttgaa aaacaacctg
     7501 catacggcca aatggagttc ctggagggtg gggtcaggga tcatgggcga aaccatcccc
     7561 aaagaatcca accaggtcac cctggataag gataaaaaag acgactgggg tattccgctg
     7621 ctccacatca aggtggatta cgatgacaac gatgaaaaaa tggtcaggga tttcctggag
     7681 cagttttccg aaatgtacgc caaagccggg tttaccaata tccgcaccag cgacagcaaa
     7741 caggccgcgg gcctggatat ccatgaaatg ggcggggtac gcatgggcca tgaccctaaa
     7801 acgtccatgc tgaacaaatg gaaccagctc catctgtgca ggaatgtttt tgtcacggac
     7861 ggggcctgca tgacctccac gtccacccag aatccttcgt tgacctatat ggctttctcc
     7921 gccagagccg cccaatatgc ggtgaaggaa ttaaagaaaa gaaaccttta atggcttgat
     7981 ttgtatcttt aattgcaaat caaaccgatc atggaacagg atacaccgac cgaattgctc
     8041 aaccacgaca gcaaaaatca cctggcgacc gcaggcacct gggcccgttt actcggaatc
     8101 ttaggcttta tattcacggc attcatcttt cttgcagcgc tgatgatgct tttgggaggt
     8161 tcatacctaa accagttcct ggccgaatcc tccggcgagg aatttccggg aatggccaga
     8221 ggcctgggca ctttcaccgg tttgttatac ctcgtgatcg gcggtattta tttcctgatc
     8281 agctggtgga ccttccgctt tggcaatgaa atccgcgaag ggctcatgac ccattctccg
     8341 gagcatatgg ctgccgcctt caacaatctt aaaaattatt tccaggccta tggggtattg
     8401 accctcgtgg cggtgggttt tatgctgttg agtgttttgg gggtcatgct ggttgggttt
     8461 ttttagttag taagttagta agttagcaag ttagcaagtg agcaagttgg tgggttagcg
     8521 attacctttg ccccatcaca aggtccggtt tcatcatgca ggcaaaggaa tggttgatca
     8581 agggtaagag ttatttatcc gaagtgccgc tggaatcggt ggacagcaag atcagccaaa
     8641 ggctccatgt aagcatgatc cgcggacgcc tccagctcac cacggatcac gtcatttaca
     8701 gttgggaaga cctttaccgc aatttcaggt atgcctccaa ccggatcaac tggaaaaaat
     8761 tcaagcccaa aaatgtgctg gtcctgggtt taggattggg ctctgtgatc cagatcctgg
     8821 agcgcaaggt cacatacccg ctgtcgatca ctgccgtgga actggatgag ggggtgcttt
     8881 acctggtaaa aaaatacagc gcttccaaat tccggtcacc catccaatac ctgtgcgcgg
     8941 atgcctatga ttttgtccag cagaaccggc aaaaatttga ccttatcctg tttgacatct
     9001 ttgtcgatga ttatatcccc tcccagtttg aaagcctcgc ctttaccaaa aagctggctt
     9061 caggcctcac tccgggggga ttgatcctgt acaaccggat tgccatggaa ccttcccaga
     9121 tcatgcagaa tatggaattt tacgaccagg cgttttcgaa agtttttccg gatgcggaga
     9181 tcctgcagat caagaccaac tgggtctttg ccagcaacaa acatctgctg aagagctgat
     9241 ttttggttaa ttttgcggcc ctgatccatt cgacatgttt accatcaata tttatttaaa
     9301 gtttgcactg attgcccttt gccttttcgg gggcgttgcc ctggcctttg cttatggatt
     9361 ctggtgggct ttcccggtga tcctcatcgg catcctgctg ctcatcagct atattttcct
     9421 gggcaccgta caatcggcgg cccagttgat ggagaaaatg gattttgccg gcagtgaacg
     9481 aagacttggg ctcacttttt tccctcaatg gctctatcgt ccaaaccggg cctattttta
     9541 tatgatcaaa ggctccattg cggcccagct caaggattat gaccgcgcgg agcagttgtt
     9601 gctcaggtcc aagtccatcg gattgccctc ggccaatgaa aaagccatgg ttgccctgca
     9661 gttggccaat ttcagcgccg taaaaaataa atggaaccag gcccagatct atatgaaaga
     9721 gatcaaggaa tacaaggtga ccgaacccat gatcaaggac cagatcaaac aatttgaaaa
     9781 agccatgcag cagcggggcc agcaaaaaca cctgatgggc ggggatcctt ccatgatgcg
     9841 catgcggaga ccgcggaaaa tgtagcctta cttcaaattc cggctttaaa ataattgctt
     9901 atccctccag cgctgaccgt attttcatta agcgctgttt ttacccaacc attgttttgg
     9961 gggaaagagg agatctgaaa taaaggagaa aggcggtgat cagagccgat aggaacaaac
    10021 ccagatcttc cgtcataccc agataaatcg cagcggtagt tcctatcaag gaccatagga
    10081 aaggaataat aaggactgcc ataggtactt tatttcccga ccataacaac ataccaaaag
    10141 tgaatatcgt tgtggggcag ggtacaccaa aagtcggaga ggcgggatac tgatgaccca
    10201 aaaggtatcc cagtatagga taaaccagga gcgcatataa aatcaggagg ccaccggcta
    10261 ccccataaat atcccactta aattgaaagg ttattttttc ccggtgtatg ccataaaagt
    10321 ataagaggcc tgcctgaacg atattcaggg caccgaataa atgggccgcc ttattgatcc
    10381 cggagaaatg aagaatatga tatactatcc cggaccagag ccataataat gatagaaaga
    10441 gaagattaat tttgtcactg atcctggttt tataaatgga gtggaatatg gccaagccac
    10501 caaacaaata aaaaactacc tgcatcggaa aaacagaaag attatatttc ctgaaaacct
    10561 ccaaaaactc gtcaatcgta aaaggcaaat tcatttgctt ttatttaaat ttaaattccc
    10621 gcatataatg gagttggaaa taataggggt caggtggaat gctgatataa ggctttagac
    10681 ctgtcggcca gtctgaattt cttcacttca tcaacttaac agccacatcc agcctctggg
    10741 ctacattatc ccaatgaatg atctggtaca agttatccac aaattccgcc cgccggttcc
    10801 ggtatttcaa gtaataggaa tgttcccata catcgatcac cagcaggggg atcactcccc
    10861 attgggttaa tttctcgtgg ttttcacatt gcaacacggt tagcttatcg gtgtatggct
    10921 ggtagccaag gattccccag ccgtttccat ccacgtcttt ggaagttttt gaaaggagca
    10981 gtttgagttt gtcgtagctt ccgaaatcac gctcgatgcg tttcagcaaa tcgccggcgg
    11041 gtgctgattt tttgttggat agatttgtcc agaaaatggt gtgcaggaca tgggaagaca
    11101 ggtgataagc gagttttttt gtccaatagt ctaccgtttc cacggcattt tcatccaatg
    11161 cttttcggac catctgcaaa tccttattgg cgccattcac cgcaccccca tggtggaagg
    11221 tataatgcag atggagggtc tcctcatcca tataaggttc gagataattt ttagcataag
    11281 gaaggggctg aagcataaag tttccctggg catctaccag tctgtcgatc ccgttctcag
    11341 cgaggttatg ggcgaagaca gaatctgaag gcaggagggc agcagcgctg agaatagtgg
    11401 atgttttgat gaattttttt cgatccatga cttaaaaaat taggtaataa agcagactga
    11461 tcacaccagc acctagaatt acgaaagctg ctgtgaattt ggtttttacc tggtatacaa
    11521 cgaacagggc agcaataaat ataataaaat tactccagcc gtttatggtc ggctggaaaa
    11581 gctgaatggc ggtaacggca atcaaaccgg taacaccggc tgcaatgcca tccaggaaat
    11641 ggtgtaaact attgttttca atcacacttt cgatgacccg atgcccaatc aaagtaaaac
    11701 aaaaggcggg taggaaaata gccagggtga tgatgaaagc accggaccag ccagcaccaa
    11761 agtacccgac gaatgtagaa aagataatca gcggtgcggg caacataccc gaaagggcaa
    11821 tgccatcgag aaattgttca ttgctcatcc atccatggca gatcacagca tcctgctgga
    11881 taaaaggaat gacggtataa gccccgccaa aggtgagcag cccgcccttt aagccggtca
    11941 aaaagacaga ccctaaagaa ctgccggatt caaggttgac ttctaatgtc gttgttgcgg
    12001 gactgagatt cgagaacata ccgggcgaaa atgaccaggt acaataagcg gttaaaagta
    12061 tggctataac tgctcctgca atccttctgc ccgtcatcca aagcgcatta gtgattccag
    12121 caaccgcaag gaccggaata aagccggtac cgcaaaaggt tgccagggcc gaaagaaggg
    12181 caagggtgat caatgatcct ttcactaaaa tatgtttccc gatccgatga acggcggaaa
    12241 agatcagtgc aatcaccgct gactgaaatc cggtaaatac tgcctgcaaa acgggagacc
    12301 cgatcccaaa actcaaataa aaccaggtta gcgccagcat cagcaaaaat cctggcagca
    12361 taaaacccag ccccgccaga aagcctcctg ttcttcctcc ggcaatcatg ccaaaccaga
    12421 cacaaagttc gtgtgcttca ggcccgggta aaacctggta aacggccaaa gcccggttaa
    12481 atcgctcact ggaaacccat ttttcttctt ccaccaattc ctgccggagc atggcgattt
    12541 gggccaccgg accgccccag gccaggcaac cgaaacgcag gaaacgtcga aaaatctggg
    12601 ccggcctttg gcgaatatca gccggtttat ccattggttc cattaaaaac ctggtttaaa
    12661 gttgaggatt ccaggtatgc ttttcaccct gaaggtgttt ggcccagctg tagagggcat
    12721 cgtagatgat catgccaatg gccagcatat cctgatcgtt ctgattattg taggacaatc
    12781 ctgcggagat cgcccacaac cctgctgcct gaggtgcaag atgaaaccgg tctgtatcgg
    12841 cgccgcgtac gattaaagcg agttggagca gtgcgggatc ggtaagaccg tgttttcgga
    12901 tgataaaatc aaaggtgcag aggtccccct cgtgcgaata ttcagcgccc ggtatatcat
    12961 aaggaatagc cccaatttcc tttgccctgt caaacacaaa tggtttcggc acataaatga
    13021 attctgcatc agggtccacg aagtttttaa tcagccaggg acaggcgata cggtctattt
    13081 tgggacgctc acgggtaatc catttcataa ttcattgatt ttaccgcaag tctgggcgat
    13141 aaaacaaaaa ataaatagaa tggaattaac tgcttgtatc gggtttacct tttttaagtg
    13201 attttttata aacagagggc tggataccgg tttgtttctt aaaaatccgg ttaaaatgac
    13261 tctgatcgga aaaaccggtg aggtaggcga tttcggtgaa ggaatagtct gaggttttaa
    13321 tcaaatgaat ggctttttcg atgcgctgct tccggatgta ttcgccgaaa gaaagattgt
    13381 cgaagtattt tgaaaaggtt ctggaaacat aggctggatg caggtcgaga tcctttgaaa
    13441 tttttttcag gcttaaactg aaattggtgt ctacataatc ctggatcgtt tccttcagtc
    13501 ctttcgccca ggccggaatg cgttttcccg gtgcaacttt tccagaaagg taagtgttca
    13561 gcatttgaat caacaattgt tcaaccgggt ctccggtgtg tttttcatgc tgcagatgtt
    13621 tcacccaata atagagggca tcataaagaa ccatcccgaa ttccaacagc ttttggtcat
    13681 cccggatgcg gcctgctaat cctgccgaaa tagcccataa cccggctgcc tggggagcaa
    13741 gatggaaacg atcggtgtcc gctccccgga cgatgacagc cagttgcagg agggaaggat
    13801 cctttaattc atgtttttta aggattttat caaaggtgca aaattcacct tcatgagaat
    13861 actccacacc gggaatatca aaaggagtgg cgtctaaaag ccgggctttc tgctggactt
    13921 caccggcagg ggcataaaaa aattgcgcct ctttatccac gaagttttta atgagccagg
    13981 gacaggcaat ccgatctatt ttaggtcttt ctcgcgtgat ccaattcatg gtttgatagt
    14041 atttcttatg gataaattta aaggggaaat tgaattatcc ctttgtaatt tcatcgtcct
    14101 aatacctgta caatgaaaag actgcatttc cttatattat tcttactcgc tgtccttcct
    14161 catgcacata gccaaaccgg ggaggaggtg tataaaaaac tgcaggccat cgccgtcatc
    14221 gaccagaaag tgatgatgcc catgcgcgac gggatacggc tggccacaga tatttaccgg
    14281 ccgaaagggg accagaaggt gccggtgatc ttctccagaa cgccttataa tttcaacagc
    14341 tggggcaacg gggaacagcg ctttaacagc tattcggcgg cctatgaggc cgtccggcga
    14401 gggtttgcct atgtcgtgca aaacgaaagg gggaggtatt tttcagaagg gcaatgggat
    14461 atccttggaa ccccggtcac cgatggttcg gatgccttca gctggctgga agcccaagcc
    14521 tggtgcaatg gaaaaatcgg gcttacagga tgttcgtcca ccgcggaatg gcaaatggcg
    14581 gtcgctgcgc aggaccatcc ggctctggcg acggccatcg cccaaggctt cggagccggg
    14641 gtgggacgca ttggcaacta ttacgaacag ggaaactggt accggggcgg agccacccaa
    14701 atgttattta tctcctggct ttatgacacc cagcacgata ttttcaggcc ttccattccc
    14761 aagaatgcca gccaggagga cctggtccgg ctgcaacgct tttatgacct ggaccctgaa
    14821 tatccccggg tggattggtc aaaagctttc cagcacctgc ccgtcaggga tatcatccgc
    14881 aacgtaaacg gccccaaaag catctttgaa tcgatggtgg cgcgcaaacc caatgacccg
    14941 gcctggtacc agggaggact ttaccatgac catatgcctt atgaaaaacc caccttctgg
    15001 ttcgtgtcct ggtacgatgt atccaccagc cccaacctgg ccatggtcaa ccggattaaa
    15061 agccaggcaa agaaagcagg ggtcgcggac caacaatatc tggtcattgc tcccaccctg
    15121 cactgcgctt ataagagggc ctcggagaat accctggtgg gtgaacggag tgtcggggat
    15181 gcgcggctgg attacgacag catgacctac gattggtttg aatactggct caaaggggaa
    15241 aacaacgggg tgctcacccg catccccaaa gtgcaatatt ataccatggg aagcaataaa
    15301 tggcaatcct cggaaacctg gccaccgggc tcttcgtcca tgactacctg gtatctgcac
    15361 agccagggca aggccaacag cgtgtttggc gacggccgcc tgtccggtca aaaaccgggt
    15421 ccctccctgc ccgcggacgg ctttgtctat gatccggctt ttcccgtgcc ttcctacgga
    15481 ggaaatgtct gctgcatggg caatgccctg gcgggtggtt cgtttgacca aagacagatg
    15541 gaaacccgta atgatatcct ggtttatacc actgacccct tgcagcaagg cctggaaatt
    15601 tccggtttta ttgaagcgac cctcttcgtc tcttcggacg ccaaagacac ggacatcacc
    15661 atcaaattgc tcgacgttta tcccgatggg aaagcttata acctggatga aaccattcag
    15721 cgcctgcgtt atcgcgaggg ttacgacaag gaggtcttta tggaaaagga caaggtatac
    15781 caggtggaaa tgacccccat gtccacgtcc aattatttcg cccccggcca ccggatccgc
    15841 attgaaatat cgtccagcaa ttttccccgg tttgatcgca atctcaatac gggcggtaat
    15901 aattacgatg aatcaaaagg agtcgtagcg aacaaccgca tccatcataa tgcggtttat
    15961 cccagtcagg tgaggctgcc agtagtcaag agtcagtagt caagagtcag tagtcagtag
    16021 tcagtagtca agagtcagta gtcagtagtc agtagtcaag agtcagtagt caatagtcaa
    16081 tagtcagtag tcagtaatca gctttcaata gacagccgtt tattctgata gctgatagct
    16141 gatagctgat agctgatagc tgatagctga tagctgatag ctggtggcta caaaggatac
    16201 ttccacggtg accggtactg ccttttcagg agtgcgttgg cggccttatc cttaaggatc
    16261 cggttgtttt gatcatccca ttcgatactt ctgcccaggt ctgccgataa catgcccaga
    16321 agggccatgt tggtggcgcg gcggccgtgg gcaatgtcgg catggggcag ttgtccggat
    16381 ttgatggact tgaggaaatc ctgccagacg aggtcaatat tttgcaggtc tggttgattc
    16441 agttttgcct cttcatgaag agtttctttt ttctcatcgt tggggaagaa ggtccatcct
    16501 tttatccagc ccatgtggaa tactccttcc gttccataaa aataaacccc gacgttttcc
    16561 gttttggttt cccggtgggg attaaaaagg ttgtggttcc aggtcaggtg aaaagactcg
    16621 aaggaataga ccacggtctg agaagtcggt gcatccgacc aggaatcccg gtcccgctgg
    16681 gtagccacgg aatacacttt tttaggcgct ttttcatccg accaccaaag gatctggtca
    16741 aaccaatgag gcccccagtc ccccaattga ccgttggcaa aattccggta ttgccgccag
    16801 ccccgcggat gcatgccttt gttataggtg accagttttg cagggccgca atacatgtcc
    16861 cagtccaagc cagccggcac gggactttgt tcctcccttt gcacccggtc gcctcctccg
    16921 tattccacaa aggctttaac ctccctgatc tttcccacct tccccgaacg caggaattca
    16981 atcccgctgg cgttgtgcgg ggaataccga cggtgaaagc cggtgatgca tattcgcttg
    17041 ttgtcgcggg cggctttttc gatggcggct ccttcgagta cggtatggct gatgggtttt
    17101 tcaaggtaaa catgggcgcc ctggttaagt gcctcgatgg ctaccagggc atgccaatgg
    17161 tccggcgtgg cattgattac gatctgcggt ttttcttttg caatgcattc tctgaagtcc
    17221 ttgtagattt taggttggtc attacacagc cgtgcgactt cctccttgca tttcgcgagt
    17281 tgggattcat cgacatcaca gagggcggct accctgactt ccccgctgcg aatggcttca
    17341 cggagaatat tggtgcccca ccagcctgaa ccaatgagta ccgtcgtgta tttggccgct
    17401 tgcttccgga tcaaaaatgg ggaagcggtg agagccaggg cgccgaggga gcttttgtgt
    17461 ataaattctt tacggttcat tggtttgagg tttgaggttt gaggtttgag gtttgaggtt
    17521 tgaggtttga ggtttgaggt taaaattatt catttccggg gagaatacct ggcttcccgt
    17581 ttttgtatct tgaaccaata tatacgggat ggaacgcagg caatttttaa ggaatgcggc
    17641 tatcggaatg gccggtacca tggccctgaa cggatggtcg tgccggcctg accaggcctg
    17701 gctggatcaa ctgatcaagg ccaatgacca ccgaattccc ggacttcttg ggaaccaggt
    17761 ccaggatgcc ctgagccccc tgctcggttc cgtgccggac cctttccaga tccatcaccc
    17821 gggaaatgcc gcaggcctgg tccaggcttt tacttgtgcc tatgtcagtc ccggatcaac
    17881 ctatcacggg gatgaaaaat tgctggcagc catgagcctt gccatgatct atctacggcg
    17941 agtccagcat gaagacggga ccatcgattt gcttacgacc aattttcatt ctactccgga
    18001 tacggctttt gtcgtggaac ccctggcgct gagttacaaa cttttatccc gcgagagcga
    18061 tggaaaatca aaagagttgc aaaatcaaat ccgggacttc ctggtgaaag caggcctggc
    18121 tttgtctacc gggggcattc atacccccaa tcaccggtgg gtcgtatgca tggcgctggc
    18181 ccggttgtcg gagttgtttc cagatcccgc ttattccaat cgagccgatg cctggctgac
    18241 cgaaggcatt gatattgatt cggacgggca atacaccgag cgaagtacct cggtctattc
    18301 cccgctgacc aaccgatgcc tgattaccat tgcccgtctc ctgaataaag aatatttgtt
    18361 tgaaccggtc cggaaaaacc tggaaatgac cctgtattac cttcatccca atggagagat
    18421 cgtgactgaa acgtcgaggc ggcaggatca gtaccaggcc cggaacgcgt cggcatacca
    18481 ttatccctat ttgtttatga gcctgaagga ccagaacggc gctttcggag ctatggcccg
    18541 gaaaatagaa aaggaaaaca gcccggggga actgacaggc agccttgctt attatctcga
    18601 ggatgctcag ctcctttcca cctatcccac aggcgccctg ccggctgaat atgaaaaatt
    18661 atttgacgaa tcgggcctgg tccgctggag aaattgggat atggatgctt ccctcctgtc
    18721 aaaaaattca actttattca ctctgtccaa taaggacgcc ctcttacagg cggtgcgcgt
    18781 ggcaaccgct tttttcggga aagggcagtt tatcgcggaa aaactggaaa aaaccgggaa
    18841 cggttaccgg tggactcaaa ctctggaagg accctatttt cagccctttc cggcggacag
    18901 cctgcccggg gatggcgatt gggaaaaaat gcccagggac caacggccca ggtccgaagt
    18961 ccagcgcatg gaatatatcc tggaggccca tcacgaaggg gatacctggt ccctcgattt
    19021 tgaccttcac ggcacggacc gggtacctgt cgcgctggaa ctgagttttc ggagcggagg
    19081 caagctgacc ggcgtcgaac cggtgcccgg tatagagcat gccttttttc tcaaaagcac
    19141 aaatggaaaa tacaccatga aaggatcaaa cctcttcttc ggccccggga agaatgaaca
    19201 tgcctggacc cagcttcgtg gtgcagaacc gaaaattccg gggcagagcg tttatatcac
    19261 cgggtatact ccgtttaagt atcaattgaa aatccatgat tgatgatgaa ttgttgaatt
    19321 gttgaattgt taaattgtca aattgtttta ggacttctca acctcaacct taacctcaac
    19381 ctcaacctca accttaacct caacctcaac cccatgcccc cgaaattatt atttatccta
    19441 tctccactaa tcctctgcgg aatggccaaa gcgcaacaaa cctaccagga ccagactttc
    19501 cagcttaaaa aatatgaagc tccgaaatcg aaacaggaat ggctcaaacg ccagaagttt
    19561 atccgtaaaa cggtctggga ttgttttggc gacctcccgc caaggccgca aaaaccggag
    19621 gtaaaaataa aatccaggga agagaaggat gggtatatcc ttgaatcttt tgaatttttt
    19681 aatggggtgg atgccacggt tccgggcatt gtcctgatcc cgaaaggcct caccggaaaa
    19741 gctccggcgg ttttgtacca gcattaccat ggcggagaat attaccatgg caaggatgaa
    19801 ttattttctt caaaattaat tccctttggg cccgggcccg aattggtgaa agccgggtac
    19861 gtggtcatgg ccatcgatgc ttacgcattt ggggaaaggt cgggaaaggg cccgaacggc
    19921 tccaaagaaa agggctccgg ggaagaactg acctgggcaa aaatgaatat atggaaaggc
    19981 cggaaccatt ggggcatgat ggtgcgggac gacctgattt ccctggatta cctggccgga
    20041 cgtgccgagg tggaccccaa acggatcgcg gccatcggcc tgagcatggg ctgtttcagg
    20101 gctttttacc tggcagccct ggacgagcgg gtcaaagtga cggttccggt atcctgcatc
    20161 acccgaaacg cggatctgat caaaggcggc gcgctcaacc tccacggggt ctattattat
    20221 gtccacgggc tgatgaaaca ttttgacacg gaaagcatca tgggctgtat cgcccccagg
    20281 accctcctca atctctccgg ggccgatgat atgctggaac ccctggaagg gcagaaattt
    20341 atcaatgaag ccctcggggg tatttataaa gtggtaggta gccctgacca gtttaaatac
    20401 aagatctatg agggggtagg gcatgcctat accccggaaa tgtggaagga gaccttagag
    20461 tggttgaagg agaagttatg agggtggaaa gtggacggtg gttggtgggg tgaaaatgga
    20521 ttttcttttc cggtaataga tccggtgatc tttgagataa ttcaggataa attcaaaaca
    20581 tacggatgat cgtccgagcg tttcaggggc catgacgccg ggtttggtga ttttttttgc
    20641 caggatgagt tcagccgcgg cgcaggcggt caggccggta gtccgggcca tggaactcat
    20701 ggaagttgcc gggtcgtatt catcgtacag gttgtaagtg taccggattt ttcttccctc
    20761 ttccactcca tcgatgaggg ttctcataaa agtgaaatcc gcctcaccgg gaagatattt
    20821 ccagtgtttg aaaaggaggg tactggtaag ctcgaggggg atgacctcct tgtttttaac
    20881 tttcaccgga tttttgtcca gcaatccgat ctcgcgcaat aaggccattt gcccggcgtg
    20941 tcccggataa cgtaaagtct tttctttcat ttccggaata tgtttcatcg tagtgagcaa
    21001 ggaacgcagg ccatcggaat tgaaggcttc cagtttcagg cctttgccgg tatggatata
    21061 ttccaggtcg gtgagcgccg gtttcaccac cactttcccc ttttctttca gtcgggcggg
    21121 gcgggtatat tcctccagga catccgccgg cgagaaaggg gctttatatt caaaggggac
    21181 ttgccttttt ttaggtaagc ctccgacata acaggcgaat ccggtcaccc gcatccgttg
    21241 atcgtggtat cccagcagga tattgtccat gcccggagcc accccgcaat caaaaacgac
    21301 aatgacatta ttttgtcttg cgagagcatt cagcttgaag gcgttctcag ggaaaaaaga
    21361 gatgtccacc atattttttc cggcttcaat ggtgtttttc atgacttcaa aacccataaa
    21421 accaggcacg gctccgatga ccaggtcggc atttttaatg tttttctgta atgcgatcct
    21481 atccccgata tcggccttaa ttttttttat gtcgtgcggc aggctgtcca aagatcggcg
    21541 gctgatatcc agtacgctga cttcatgttt tgcggccatg atccgcgcga tggcactgcc
    21601 gaccatgcca cagcctagaa ctttgatcct ggacatgtct gatttttttt taaaaataag
    21661 ctaattcctt ttccggaatg attttcggga attccgataa tttggaatca ttaaccaaaa
    21721 tggtatccgt tatgaaatcc ttgttgcctt cagcccaact tattttcctg ttcttagcct
    21781 gcatgatcca acagggatct gcacaaaggg ggaacggccc gcagcaattc atcgggatta
    21841 gtaaaaaaag cccctcatat ttttcctttg ctgacggccg cccttatgtg cctgtcggta
    21901 taaatatgat caatccgagc ggccggaatc ataataatgc agattccgcc ttccaggaga
    21961 tcgaacaatg gatgaagaac ctggctgaaa acggaggcaa ctatgtgcgg atatggctga
    22021 gccagtcttt ctgggatatg gaagatcagg cggcgggcaa atacaatgaa gaaaaagtgc
    22081 accggatcga ccgcttcgtt gccatggcca gaaagtacgg gctccggatt aaaatgaccc
    22141 tggaacattt caggagcatc acgatggagg aaaatcccca gtcctgggcg gtgaaagggg
    22201 tctaccataa atcgaagggc ggccccctgg acagcatccg gcaatacctc agcagcccgg
    22261 aaggacggcg gttgttttta aataaaattg atttttatca aaaacatttc gggaacgata
    22321 ccttgttttt cggctgggag ttgtggaatg aaatgaacgc ggtgaaagga ccggaagacg
    22381 acctgttttt tgaatggaat gaaacgatgc tcagggaagt gaaacaacgg tttccggaaa
    22441 acctggtcat gcaaagcctg ggtagttttg atcatgaaaa cgtcaggccc gtctataaaa
    22501 ggatgatgtt gcttcccggg aatgaagtag cccaggtgca tcgatacctg gacctgggag
    22561 cgaaaatgga gatatgccag gccccgatgg accttattgc ttcttcggcg gtggacgaat
    22621 tggtgactta ccaaaccggc aaacccctca tcctcgccga gacgggcgcc gtagaaccgc
    22681 gccatacggg tcccagcaaa tattacccgc gggataccgc cggcactcta ttgcacgata
    22741 tcctttttgc ccccttcttc tccggatcgg cgggagccgg gatggcctgg cattgggaat
    22801 cctatgttca taaaaataat ttgtggtacc attttggccg tttccaagag gccatcaagg
    22861 ggattgaccc cattgaagag ggctttgttc ccgtgagaac agaaacccat gcggtgcgca
    22921 tttatcatct aaagggcaaa aagaccagcc tgttctggat cagggataaa tcaagccact
    22981 gggaatcaga actggaaaaa ggaatccttc ctcaatcccg gaaagggatt aggttgaccc
    23041 ccaatgaaac cggattgccg cgaagagccc ggaccatcga agtttatgat ccgtggaaaa
    23101 atcaatggtc ggtggttaaa aagaagggca ccggttttac cttaccggat tttaaaaggt
    23161 ccctggtggt aagggttgtt cattaaaggg tcccgtaaaa gcgaagggca aggtgaaata
    23221 atttctttac tgtcctcagc gtttgatggt tttaaggccg gagctccgtt attatcaaac
    23281 cttgaggcag ggatattgat taccggtcgc tcaaaccccg ggaggcaatt tttcaaacat
    23341 ccgccggcgg taattgaggg tccttccatc cacaataaaa aaaccgtccc ctctcgaatg
    23401 gatggtgcgt cgctccttcc ggtttggctc aatccatgcc tgaacggctt ccaggataaa
    23461 cagtttgtgt tttttaaggt gatggaccac gcggcactcg atattggcca gacattccct
    23521 gatcaggggg gcatcgacct gcttcgcctc caaggccgtg agcctgaatt ttttaaattt
    23581 atcggtgtcc gccccggagc acatgccaat gtcaatgact tttttggaaa gatccagggc
    23641 tggaatcgcg aggacacatt ctttcgtttt gaccagggcc ttataagaat aattccaggg
    23701 ccccgtcagc agggcaaaac gtggagtgaa atccatgacc atatgccagg aaatggtcat
    23761 gatatttttg ctccctttat ccgccgtcgt gaccagcacc acagggccgg gttccaggag
    23821 catgaaagct ttattcaagg gcagttcgtt catcttttta ttttaaatat aatcatcgat
    23881 tttggaagct ggaagttaga agttagaagc tggaagttag aagctaggta tttccaatcc
    23941 tttttctttg cgtcatgccc tttgcgagag ccgggtctgt ttctccaccg tattagtctt
    24001 cacttcatca ccgcaatgcc tcccggagat ccggattttc aatgatttca atggctttct
    24061 ttatgccgtc ttcttttttt atttcttcag cgagccggag ggctctggca tggatctccg
    24121 gattgctgat caattcgcgt atcccatcgt tcaggagatc ctgggtcaat tttttcagag
    24181 gaagcggttt taaggccact cctttattaa aggcaagggt cccccaaaaa tgctggtcgc
    24241 ccagaggata aaggacggga caggtcataa agggttttcc ggcctgcaaa caggcggcca
    24301 tggtgccgat gccgccgtgg tggaccaccg ctctgaccag ggggatcaat ttgtcataag
    24361 gagcggaatc cacgaacaga atatctgtat cggaattttg atctgccgaa gacgtgatac
    24421 cccagccttt gatcacaaga aggcgaatac cgagcgtttc cctggccgat ctcagggctt
    24481 cgggaaggtc cagtctgctt tcaaaaggca tgcttccaaa agtaatgagt acgggggctt
    24541 ccccggcctg aataaaatga aggatctccg ggctcagcgc ctcatcactc ggcgctgacc
    24601 agaaaccggt gaaatgggca ttggccggat aatcatccgg tttttttaaa aattgagggc
    24661 taatgccgta gagggaaggc agggagggtt ttataaaatg cacgggtaac ccggctttta
    24721 cccggaaatc gtcaatggct tttttccaca tagccaggcc ccattcggtc aatttataac
    24781 tgaatttatt caggaaccgg ggcaggggca tcgcgctgaa cacaggattg ggaaattccc
    24841 gggtggcctg gcttgcggga accacgttgg ccatgatcat ttttccggga aactgatccg
    24901 agaaattttc agccatggtt ttcacatgaa ataaaacaag gtcgctcctc tttgaaagtt
    24961 cataaaaagt gacgaatgca ttggccagca tgggaaagac gaccttgcca atctctttcc
    25021 gggcacgaag cgggtttttt ttcatctgtt tacccgcgtc ggaatggatc atagcctgga
    25081 aatctgcttc tactggtaca aaaccgagtc cgtaagaacg caccagcgat tcgaaatttt
    25141 ttcccgtgga aagggtcact tcgtgcccac gtttggtcaa agcctgaccg aggactgcaa
    25201 acggttggac gtccccccgg gtacccaggg tcaggattgt tattttcata cttaatttag
    25261 ccttcgatgg aatattccca aatatacccg cactgggcat gaatccggcg gaaaaatcag
    25321 cctggataaa atcggaagcc ctgaaatcag gatttctaac ggcgggcatc gctcgcgcgg
    25381 aacccttgaa tgaagagcaa aaacaactcg aagcctggct ccggaaaggc tatcagggag
    25441 acatgtctta tatggagagg aatctggata aaagagtgga cccggccggg ctggttcccg
    25501 gagcgaaatc cgtgatctcc ctggcctata attattatac cccggaccgg ccggtacccg
    25561 ggtcattgaa aatatcgacc tacgcctatg gccgcgatta tcataaggtg ttgaaaaaaa
    25621 aattaaaagc gttgtggaat tcgatccgga tgcagctttc tccggatgcc caggcaaggt
    25681 attttgttga ctccgccccg gtgatggaga ggcaatgggc ggcccgggcg ggactgggtt
    25741 ggctgggtaa aaacaccttg ctgatccacc cggggcatgg ctcctttttt ttccttgccg
    25801 agatcatcac tgacctggaa ctggaatatg atgagccgac gtgggaccat tgcggaacct
    25861 gcacccggtg catcgatgcc tgcccgaccc aggccatcgc cccggccgga tatgaactgg
    25921 atgcccgccg gtgcatttcc tacctgacga ttgaaacaca gaatgatatt ccggaagaac
    25981 aggtgcagca catggagggc tatattttcg gatgcgacat ctgccagcag gtttgtccct
    26041 ggaaccggtt cagccggccc catgaagagc ctgaatttga gcccgggaat gattttttga
    26101 attggaacag ggaccagtgg ataaagctgg atgaatccca gtttgcggaa accttcgggc
    26161 atagtccggt aagacgggcg ggctatgata agttgaaaag gacgattggg tcagtgatga
    26221 aggggggcaa ggggtgaggt atgttccacc ctcaagatag aagctcgaag ctagaagtta
    26281 gaagctaggg cgttttggca tggcagttcc gggaaataat agacgctccc ccagcttcca
    26341 gctcccagct tccagcttcc aagttagaag ctcgaagcta gaagttagaa gctcgggcgc
    26401 tttggcctgg cagttccgga aatttagtag acccacccca gcttccagct tccaacttcc
    26461 agcttccaat ttagaagctc gaagctcgaa gttagaagtt agggcgtttt ggcctggcgg
    26521 ttccgtgaga tttgtagttc ctccccctag cttccagctt ccagctccca gcttccaagt
    26581 tagaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcgt tttggctcgg
    26701 cagtttcggg aaatagaaga cccaccccca gcttccagct tctagcttcc aactcctagc
    26761 ttccagctcc tccttttttc ccgcaaacac tcaaagacca gcccggggcg tatcttagca
    26821 gaggcatgat cctagtttgt tatggcacga ggccggagat catcaaaatg tcgccggtca
    26881 tcgaagaatt gaagaagcag gaactccggt tcaagaccgt gttcaccggg cagcataagg
    26941 atatgtatga acagtttaag cataccatcc gaacgccgga ctactggctc gatgtcatgc
    27001 gcagcaaatc ctccctgggc gatacgatag gctccatcat gcacaaactg gaccttttac
    27061 tgggcaaggg aggcatcgac ctggtgctgg tgcagggcga ttcctccagc agttttgcct
    27121 gcgccctcag cgctttccat cacaaggtcc ctatcgggca tgtggaggcc ggcctgcgca
    27181 cctttaatat tcaaagccct tttccggagg aagggtaccg ccagatgatc acccggatcg
    27241 tcaacctgca ctgggctccc actcaaaaag ccctggacaa cctgctgagg gaaggggtgc
    27301 aaaatgcaag gcttaccggg aataccatca tcgatgcctg ccttggtacg cagaatgaga
    27361 tccgttatgg caacaaggtg ttggttgtcc tcaaccggag ggacaattac ggagagcgaa
    27421 tgaaaaaatt gtttaccgaa gtggaagagc tggccaaccg gcatccggaa ctggaaatca
    27481 ttttccccat gccccacaat cccaatgtca agtcacaccg ctatatcttt aaaaaagtca
    27541 aggtcatcga gcccctggaa tacagcgaga tcctgaagct catcagtgaa gcgaaatttg
    27601 tgatcagcga cagcgggggg ctgcaggaag aatgcgccgc cttcagaaaa aaaatcctgg
    27661 tatgcaggga aaatacggaa aggcccgaag gcctcgaagc cgggatcggt aaattggtca
    27721 ataccgaggt ccttcagcat taccgttggg cgatggatca acccgaatgg acaggcgaga
    27781 atccttttgg agacgggaat gcgaggatca ggatcgtgga gcacatccgg gagtttttag
    27841 ggatttaata aggaataccc ccttcttgcc cctacccccc tttcgctccc tttcgctttt
    27901 tgctccttta gcccttaaac cttaaccctt aaccttaaaa ttgcagggga taccctttag
    27961 gccaacccgg agggccggat ttccttactt ttgcaccctg ttaccacatc cattcaatcc
    28021 acaacatgaa tatcaattcc aaattttttc cttttgcatt attgttttcg gcaattgcct
    28081 tgttttggac ctgtaagcag gaagaagaag gcccgataaa gaccctgatc accggggaga
    28141 tcaccggtta cccggacggc accctgacca tttaccgggt ggtggatgaa gattcgagcc
    28201 gggttgacac tttcccgctg agagatggaa aatttgagat cagtctgtcg gatcatgaac
    28261 cgaggatggc caacctggta tttcccgacg gcatgaccgc cgtacagttc tttactgaag
    28321 ccggtaccat aaccatgaag ggtaccaggg aagaagcggc caacctggag gtttccggaa
    28381 ctcccgcaaa cgagctcaat aaagaaatga aagccattta tgccggcttt gagcaaaggt
    28441 ataatgacct gatggaacag gcgaccaagg cgcagcagac tgaggaccag gccgccctgg
    28501 ataccctcga gcccctcctc atgaagtttg aaggggaggt ctcccggagc catgtcgatt
    28561 ttgccatgaa acatcccaat accatcctca gcgcgtacct tggcctgagc gcgtccatga
    28621 gccccggcct ggacttaaaa cctttgtatg aaagcttgtc cgatgaagtg aaaaatacct
    28681 tttttggtct gcgattgaaa aatgtggtgg aatcggccgc aaaaacagcc attggttccg
    28741 cagcccctga ttttgaaggc atcagccccg acggaaagat actgaggctc tcctccctga
    28801 aaggcaaaca tctcctggtg gatttttggg ccagctggtg catgccctgc cgccaggaaa
    28861 acccaaacgt agtgcgggtt tacgataact ataaaaacaa aggatttgag atcctggggg
    28921 tctccctcga ccaggaaaaa gaaaaatggg tggatgccat cgcccaggac ggtttggcct
    28981 ggccccaggt atccgacctc ctgggatgga aaagcaaaat agccgccatg tacggggtgc
    29041 agggtatccc gcaaaacttt ttattggatc atgaaggcag gatcattgcc aaaggcctgc
    29101 gcggacagga ccttgaaaac aaattgaaag agttgttgaa ctgaggaact cataacctga
    29161 tccagctttt ccatgttcgt ttcgattaca gactacctgt atgaattcaa ggcgaactgg
    29221 gcggtaggct tacaggataa aaaatttttt tacacgctgg cgggcatctt tgccgcactg
    29281 atccccctgg ggctttacct cgttccctat ctgcaattca tcgaacacag gcagggggtg
    29341 attttaaatg attttttgct ggatcaattt ccaagggcgg attattccaa tctgatcttt
    29401 tccattatct atacgtcggc ttgcttcatg atcctgtatt cccttcgttc accctggatg
    29461 ctgctccgga acctattgat cttcatcgtc cttcagtata tccgaaacct ctgcctctat
    29521 ttcgtccccc ttgctccgcc ggagggcatt gtgcccttga gcgaccccgt cttggagatg
    29581 gtggcataca accggcaacc cctgttgcag gatttatttt tcagcggcca tacggcaact
    29641 tgtgtggttt ttgccctgct cacccgaaac catactggcc ttttcagtac ttttatcgtg
    29701 atcacggtcg tcatgatctg tttattgatg atccaacatt gtcactatac gatcgatatc
    29761 ctcggaggta tcgtatttgc cgttctggct taccggctgg tagaccggtc gtggctgaca
    29821 atgaaactgc cgttccgtta attcagaaat aaaaaaacct cgaaccaaac catatgtaca
    29881 gcctggactt aaaaatcaaa acccttgccg atcaggtcga ttatttacag aaccacaata
    29941 tttatcttta ttaccgggag atggattcgg cacaggatgc tgaaatgatg gcggatggca
    30001 gaaaaatcct gatgttcggt tccaacagtt atctcggact caccaaccac cccaaagtaa
    30061 aggaggcggc catacaggcc atccgggaat acggaaccgg cgtatccggt tcgagattgc
    30121 tcaacgggaa tcttcggatc caccgggaac tggaagaacg attggctgct tttgtcggca
    30181 aggaggatgc cgtggtagtc agtaccggat tccaggccaa cctggccgcc attcccccta
    30241 ttattgaccg ctccggttac ctgatcatgg acaaactcag ccatgcctcc atcctggaaa
    30301 gcgcggcctt atccttcggc cgcaagtcaa agtttgagca cgatgacttg aattcccttg
    30361 aggataaact cagtatttgc ccggcggatg ccaccaagat ggtcgtaacc gaaggcgtgt
    30421 tcagcatgga tggcgacatc cccgacctgc ccgggctgac cgccctggca aacaggtatg
    30481 gagccctcgt catggtcgat gatgctcatg ctctcggcgt cctgggccaa aacggatccg
    30541 gaagttcttc tcatttccag gtgaccgacc gggtggacct gatcacgggt actttcagca
    30601 aatccctggc cgccctcgga ggtttcgtgg catccagctt cgaaatcatc aagcttatta
    30661 aacatacttc acgatcgcag atgttcagtg caggacttca gcccgccagt gtggctgcgg
    30721 tcatggccgc cctggaagtg atcgcagagg aaccggaacg gatcgaacag ctttggaaaa
    30781 atacccggta tgccatggaa ctgatgaaac aaacaggatt cgacaatgga acggctgaaa
    30841 cgccaatcat tcccgtatat gtccgggacg atgataagac gtatttgctg gcccgccggc
    30901 tttacgatga cggaattttt gtcaacccgg tagtctcccc tgcgaccagt ccggaccagg
    30961 cactcatccg cttttccata atggccacgc atacgaggag ccagattgaa gaagccattg
    31021 ataaaatgca ctttcacgcg aaggccctgg aaattcttta atgcaatata tgattgagat
    31081 caagttggtc cggaacaaaa gggaattaaa gcgttttatc gacttttcat acgacttgta
    31141 ttccggggat tccctcttcg ttcccgaact gttcatagcc caaagggatt acctggatcc
    31201 ttccaagaat ccattttttg aacatgcccg gcttgaattg ttccttgcgg aaaagggggg
    31261 taaagtggcc ggccgcatag gagtagtccg ggacgaagta ttaatagaat ataccggcga
    31321 aaaaaccggg gttttcggtt ttttcgaaac gatcgaagac gaagaagtgg ccaccgcgtt
    31381 gttggatgcc gcggcggcct ggtgccggag tcagggactg atctgtttgg aagggcctta
    31441 taatttcagt accaactacc caagcggact gctgatcgat ggttttcaat attctccggc
    31501 cgtcatgatg acctacaata aacgctacta ccagggattc cttgaaaatt acggatttac
    31561 aaaaaagacc gacgttctgg cctatcatct ggatgctgac ctgttccctg accggttgga
    31621 aaaaaacctc ggtatgctgg aaaggcgcct tgccgaaaag gggattaccg tccgcaaaat
    31681 tgatatgaag catttttacc gggacgtgaa aagtgctata aacgtttaca accaggcctg
    31741 gagcaagaac ctgggcttcg ccccttttac ggaaaaagaa ttcatgcatg tagccaggga
    31801 tctgaaactg atcctcgatc cggaactggt cctccttgct gaaagggagg gtcgggtcat
    31861 cggcttctcc ctgtccgtac cggatatcaa cgaggtgcaa aaggggatcc gccgaggaag
    31921 gctgctccct ttcggaatct ttaagttgtt gttcaacaaa tcaaaaatca agaatgtccg
    31981 catccttgcc ctgggggtag tggaagaatt ccgcaaactg ggactggacg cctattttta
    32041 tgcccgggcc ttttcttatg tccgcaggca taaaagactc caggggggtg aggccggatg
    32101 ggtgctggaa aacaatgcgg agatgaacca ggccttagtc aaaatgggag ggaaggtccg
    32161 caagaaatac cgattttaca gattgaacct tggaaatcat aagaaccctt gagattatca
    32221 ggaattgaat ttgaccggga cgcaaccatc ctgctcctca gcgcaccggt gctgttgagc
    32281 atttattatt atcatggttc ggcggaatct ttcttccggt attttaattg ggctccggaa
    32341 ggtgttgggc cggtttattg gcggtttttt gtgtttttcc ttctgcttgg cattattccc
    32401 ctttcgatca tattctgggt tttcagggac aagccatact cgttcggatt agctctaggg
    32461 gactggcgaa agggattgag cctggcctta atccttatcc ccctgttgat cctgcccctg
    32521 gcatggggtg cgtcacaagt tccggaggtg cagacagaat atcccctggc aaaagtcttg
    32581 ttcagccagg gccctttgtt ttggcaatat tccct
//
LOCUS       DAOQXT010000046        32608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1590_length_32608_cov_6.450680, whole genome shotgun sequence.
ACCESSION   DAOQXT010000046 DAOQXT010000000
VERSION     DAOQXT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 32608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32608
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_32608_cov_6.450680"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            702..1757
                     /locus_tag="PKM27_09700"
     CDS             702..1757
                     /locus_tag="PKM27_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07577.1"
                     /translation="MNALKIPNTAIFGGREAFSISNYADQSIHIFDFRARNHLLAIQL
                     YTEGPNTTISGKYGFYHQLLPDQTVLILDPPTKRLSRVDIRGTRLESHTIPPDPDFEY
                     LAVVDWSIQGVLYGDDFFYPVIPGGFMPLTIQDPQYKRLKHLLQYRVGEGFRSMIIGR
                     NNFYTHERFYNFDECCMTNFLSFLKNDLLISDCVNPFVRIYEYGTGRLKDSIEMKSRE
                     FDQVPAYDQPVTAGEYQHMPANLYREILEFGHTHPSYYQLHVDTTHHLIIRQAKLGMT
                     DEEYMDNRFYWSHSFILSDTDHFQYRGEAIVKRGDYKNISFLNGAMTSEGLYFPFFDP
                     QGMEEDHLYFLRARFIE"
     gene            1891..2286
                     /locus_tag="PKM27_09705"
     CDS             1891..2286
                     /locus_tag="PKM27_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07578.1"
                     /translation="MKHIISFVLSCYSFYICNGQINNFNINSLTRDPTTLDSISFSAG
                     AISEYVESNYFLFSDSALIDGPNIKIRSNEMKVYYLKEDHPRQKFFTEAHICGIAYFF
                     INKEQINLPIGYKKCILDLSNLNYIFLFE"
     gene            complement(2457..5489)
                     /locus_tag="PKM27_09710"
     CDS             complement(2457..5489)
                     /locus_tag="PKM27_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR07579.1"
                     /translation="MIRYLIRRPVAVFLSFGLLLVLGLYYFRSIPVSLLPPIDVPRII
                     ITLSYPGHTASQVESSALRSLREGLLTTSHLVDIQSRASNHRGSLYLQFEHGTRMDLA
                     YVEVNEKIDRLSQSLPRDLPRPQVNRVNTSDIPVVRLHVLPDSSGPELATLTDLAEKI
                     LKKRLEQLEGVGTVDINGQVENIIVVQPDQQALQAAGLQTADIGTAIRNANQELGSLS
                     IRDGQYRYFVRLAQTLHDPETIRKIPVQARQGSIPLDRLCRVDLVPEPRLGYHVYQDE
                     EGLVLTIKKQAGARMHELMPRLYDAIGLLRKDYPGVRFEISQDQSFLLVAGIDNLKQD
                     ILYGGILTIALLFLFLGHWSAPSLMSISIPLSLVIGVVFFYLFGLSFNIISLSGLALG
                     IGMLIDNSIVVIDHISRKRKSGLSLEESAVSGTVEMAPPVISQVLTTVVVYAPLVLLH
                     GMAGDLIGDQAKALTISLFVSLLIAFGLAPLLYKVLLSRLSRGEIREDTVFYRWVEKG
                     YHRMIRHILRYRPIYFVLTLLMMPAGFWIARFLPVRALPETQKTESLIRIDWNEPIDA
                     GENRRRHGLLTEQIRPFILASEADIGITQYLLQDSRQTVQQGSLYYACSSDADRSKAD
                     EAALAWVRTHFPAASLELEEAPNAFTQLFSSSVPYFEARFTNVTRGQDSLISGLAEKM
                     ANTGALPGEGLASETHLEILPDYPKMALYGIDRQTLDQVLEQHYGSYKLTELQNASDR
                     VRVNLVSERNENQAPWLRSSNGQSYPLSLFVQFRYRSQPKQILADRSGQYHNLIWPDG
                     TGSTVEELKGRLSVFALDQGIPVHFSGRYFEDQKQLGQLVLIFLLVVLLMYVILAIQY
                     ESLVQPILVMLTIPLGITGSMLLLWATGSSLDTMAAIGFIVILGLIVDDPILKIETLN
                     RLSKAYKSQGLPLNEETLSRMIHEAGGICLKPLLMVSLTTSLALVPVLFIPGLGNELQ
                     KPLVWVIIGGLTIGTFFTTWFIPLAYWYWIRMKGKFMN"
     gene            5772..5969
                     /locus_tag="PKM27_09715"
     CDS             5772..5969
                     /locus_tag="PKM27_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07580.1"
                     /translation="MEHEKFYSKHDLAFELGISLKTMGQRIKKLENGVVKELYREKRL
                     FSPAERKIILKSLLDHVPSNR"
     gene            6603..7559
                     /locus_tag="PKM27_09720"
     CDS             6603..7559
                     /locus_tag="PKM27_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07581.1"
                     /translation="MLIEDGVSSSLIILDIPLTGLELPLKSGKIQSINIYKDKLIIKE
                     SNGSHILTVYSLKEGTYTGYGSIGEGPGKIPNPEFCKLVHVEEGGSVMIFDFLTKKIY
                     RLSLMDNTLAPLYTLSREEGAAQSGIWLGDSILALADPAGDPIRILDLKSDKSIPASL
                     ELPFRRTLTPEERMQWYPKDLAFNTKHRLLLTWSIVSNTLSVNSTNGYNLKDYQFGAN
                     QNLDDNQIHRNKIYYYDGKTSGDWVYALYGGFDLNGQFLNKIISHIRSELHLYDLKGD
                     TLRRFRLDRLVNTCAIDFTGNCIYCIQEGGKEDKPLVKYEMD"
     gene            8302..9504
                     /locus_tag="PKM27_09725"
     CDS             8302..9504
                     /locus_tag="PKM27_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07582.1"
                     /translation="MNKFIISVFFFSLLTCKLDKKEKINNDPIKKYEVSFDTLKFLSP
                     AIHYSSASLFKVVNNSILNDIEVMSISNFINNTIQIYDFKKRKIYKTIQLHKDGPDRT
                     LTGQFGFTHQIIPGNKILVLDPLSYTFSVIDLNGHKITSQRIPPDPNFEYAAAMEYSI
                     QGVLHGDDFYYPAIPAHYAPLSNKDKLYDKLKHLIHYNTNTGFQLPKINRNNYYTLDL
                     IYNTAQCCYANFVTSVNNKIFVSDCYQPDIKCYDARSYKYLYSIPAHSGYFYSAPSYD
                     EHLSEQEYLFPPAETYRSMERWGFVHPSYYQMYIDTINHSIIRQAKLGMSDEPYYRDR
                     TQYTSSFIFIDYDTNKIKGEALVKGGMIEKVAFFNGFITSEGLCFPFIDIQQNEDDHI
                     YLVKAVFH"
     gene            9985..11046
                     /locus_tag="PKM27_09730"
     CDS             9985..11046
                     /locus_tag="PKM27_09730"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNR07583.1"
                     /translation="MDSSLGTDIADLFLSVEYLPLSKSPNPFHSIDQLRISADWIYLL
                     ISQAGEGQSEVMIFGRDDGHYFRSIKGGFSGPYDIRFISCIDIYKNRLYVMDPLQSRI
                     FVYDEQGVRLTGLSVPDEYRKFAVLNEHVLILDSDEAPTASGTRLLYFNTQNGKSMPL
                     RHNGKVQSGMAWERFFRKSNSDLLFYTAHDPNVYQIKESGSDTLYRFDFGTRLILESD
                     IPPNINTKLTMLNDLMDEGRRFTTMFSFMDFNSTLFFTYYLNNHMRFVRVAIRDSGYR
                     TDQFEFKRNTLDGGPLPDLPPLGYTDFGEMVFVYDAEYVYHLCQDKSGNKRKSPDLIA
                     LCNKIKIEDKPVLAFYRMK"
     gene            11054..12361
                     /locus_tag="PKM27_09735"
     CDS             11054..12361
                     /locus_tag="PKM27_09735"
                     /inference="COORDINATES: protein motif:HMM:NF025336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4221 family protein"
                     /protein_id="HNR07584.1"
                     /translation="MKYLILFVLNFFSFLNCNDPVQKIKKVTLINFLFFSLFMVAILT
                     LFGCKKPLNNEVIEVKTINNFRIDTIFLLAANSKFVNPRTYKIVNNNYFMDTEVTSIG
                     NIVDKKLYIFDFKERKLVKTIQLYSEGPNTTIYGGYNVYHHIINDNTIIIIDAPSGKI
                     SRINSEGIKLKSFDIPVVGNNSYGLFIDFMIQGVYANNLFLYPVAPWGVFPETVEDLN
                     VLDLFHLLSIDFNKNHVNFKIKRSNLYTLDKLYYLENCCQTNFIANNRDVELFISDCF
                     NPEISVYSLIGLNKKTNFIMKSNEFKNVPPYYKKFTSSEFSNLPAQSFREILEFGHKN
                     PSYAQLHVDTFNNLIIRQAKLGMSEENYYEDRTKWAHSLILHDLTTLKFINELKINKD
                     EHLDINFLDGFMTSEGLVFPFKVIQSKENEDILQFAKITFLPE"
     gene            complement(12405..13475)
                     /locus_tag="PKM27_09740"
     CDS             complement(12405..13475)
                     /locus_tag="PKM27_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR07585.1"
                     /translation="MNIRHRNLFILLYFLNFLGACKTPADPSEPPLVNSTRDTTATPV
                     RTALCTEGWFEYRVEANGIFKAEKALKLTAETNGRVLNTRATNGEVIQAGEVILQLDT
                     IDLASRRAALEIKRYLAEKEFESQLLGYEKLFLGMDEAEKLTVRKKLHISSGLALAEL
                     ELKDLEQTIRKAVILAPFSGKVADTKHLAGQYVQAGNELFTLLSHSLLIECQVLEADL
                     PQIRIGQSADILPVADPGSTVKGMVHRINPQVDQNGMSLVAVKVQGSWNNKLYPGMHG
                     LAVIKIPVRKSLQVPKEALVVRGNRQVVFTVENNQAKWNYVEAGRNNGIVVEILSGLK
                     PGDRVITTNNLQLAHEAMVMYE"
     gene            13573..16701
                     /locus_tag="PKM27_09745"
     CDS             13573..16701
                     /locus_tag="PKM27_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR07586.1"
                     /translation="MVSPFRTVFIFIILGILGGFSIPYLSIDLFPKEASDRLKVQVAL
                     PGHPPELVESMVTQALENAFSGLPGLKSLNSVSYYDRADIELIYNKGRDLDLARFELA
                     AMLRRVFPGLPAGASYPEILAHGGNTDAVNRFEAPFLIYSIRAPAQPFSIRNEADKLF
                     RKEFAGVPGLKEVKITGTEDLQLVIRYDLKQCGAYGISPGEILNAVGKQYRVSYPGVY
                     TDQDGSAYFLKVGRAETGLSGLENLILPAGDQSIKLRDIAEVFIEEQPVQGHYRVNGQ
                     NSVNLMFFAQEGSNRILLAREIREKMASMADLLPSGYRADLVKDETVFLSEELNKNYK
                     RSLAALAVLIVFLILGYRSWRHLVILILTLVINLALTALACRILSIPVHLYSIAGLAI
                     SFGIMIDNSILTLDYFHQYRSRHVFLAVLGATLTTVAALLFVFLLPEEMKRNLVDFSA
                     MVSIALVVSLAVSLWFTPGLYQLFYPESRQADRRSGMEGTGLSGVTLFYFNSIRLLAR
                     FRKTFLVSVVLMFGLPLFMLPSSWPGKEWYNKTIGNTWYQEHLRPTIDRITGGALRLF
                     VREVYERSGYRDPQRTRLYISAQLGPGNTLDQLNVILSQFEIYLKEVEGIDRYVTSII
                     SGQYGFIEISFLPEFEHGILPWRLKSQLTSKATDWSAVEWGIFGVGQGFSTMRQDQIP
                     LFRVLMKGYNNRELEHQAQLLKERLLAHPRIQKVNTNERLNWNEKASLEYLLTLDEQA
                     MAAGRMDKQSLFSSLRNLSQPISTGQVIELGDKSYPLVMREAGTQEYDLWDLKNKALQ
                     GDTARAFKLGKLSGLALVPTASAVHKEDRQYIRLVAFEYLGSEYFGSKYLDQVLVEMK
                     SLLPPGYEASRKRGRWGYGSQAKPYGILLLIILANFFICALLFEHLVLPFYIILMIPV
                     SFTGLFLTFAWGDFYFDQGGYAAFVMLTGLVVNAAIFIVHDYQQAVKQNPGLPGNSLL
                     ILVIANRSRTILLTTIAAASSLLPFIWEGQNEVFWFSLAVGVLGGLSFSMFAVFIALP
                     VLIWNHSESFPK"
     gene            17033..18043
                     /locus_tag="PKM27_09750"
     CDS             17033..18043
                     /locus_tag="PKM27_09750"
                     /inference="COORDINATES: protein motif:HMM:NF025336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4221 family protein"
                     /protein_id="HNR07587.1"
                     /translation="MDTGSTINIDVKDALNSYKTSSTFLNLVVAYNGMNHSLDLFNIS
                     DKSFIASHKLESDGPFGLGIIRKLHFYSTDSIFIFSENKVFICDTSFDHCSAIPLPGE
                     TNPVSGFNPERYYIHWGLRGVNPFYFKGSVYLPCIKLDEKTEAGLKPLCCKYDIPSGS
                     AEMIDIPEEIKGPDTWYGILDQVNYSLFDSFLYFGFRYSPDIYRYDLNSKIFQPVYLS
                     DIGENPPSKRDGSMDYPVFNIMNYYTYHELKITPGGTLQAIFNKPGKESPKTGRKVIN
                     EMFLRAIHLADSHKSAEELLILSKLDPFKGILYNNAIYFPLDKPDSENLMRLHGYELV
                     CK"
     gene            complement(18091..19182)
                     /locus_tag="PKM27_09755"
     CDS             complement(18091..19182)
                     /locus_tag="PKM27_09755"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR07588.1"
                     /translation="MRILIFLLCIMSLPVRAQSLLREEIGKLMQREWDQFDFSGVLLV
                     AREGKPVYYKAKGYRRFNGEHKLRKTDLFEWASISKSFTSMVILQLKEVGKLNYEDSL
                     GKFIPGLPYHGITIRHLLTHTSGIPDYQEIMDQYWDKSRIAGNEEVIRYLKQYPRPML
                     FPPGSKYAYSNTGYLLLASIAEAVSGRSFTEWVNERIFDPLKMHGAGFRNPGSRSRIK
                     NFAWGHIYEEDKKRYTHADSFPAFNYTIWLGERMGPGRISGSALDLLKWDRALYTGKP
                     VSQKILEEAFQPYRLSDGGISNYGFGWALRTHPTLGRVVHHSGDNPGYRTHIVRYIDK
                     KYTVILLNNTSSGRMEDILKELEEMLVRN"
     gene            complement(19182..19877)
                     /locus_tag="PKM27_09760"
     CDS             complement(19182..19877)
                     /locus_tag="PKM27_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07589.1"
                     /translation="MAVRLFNIPITPWRWLAILVSVVFHPLWWPSLGLYLLLVLNPFL
                     FGVNEASARTSLLLQVFALTFVLPVFSIFLMRKLALINSYQLTERLDRIGPYMVTMIF
                     YLWLYLSIRHDAAVPLVYNIFIFGALITLGLVFVSNLFFKISAHTSVMGGVVAMTWLT
                     FNFFSHETFTVDLPGEGLASLSWYSALVWVLLLAGLVGTCRMFLRAHTLREIYLGYLA
                     GFVSQWIAFKILF"
     gene            complement(19877..21829)
                     /locus_tag="PKM27_09765"
     CDS             complement(19877..21829)
                     /locus_tag="PKM27_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HNR07590.1"
                     /translation="MDVLKGFYRELLRPRQIEEKMLNLLRQGRISKWFSGIGQEAISV
                     GAALALQPEEFIFTMHRNLGVFTARKVPLGRLFSQWQGKAGGFTKGRDRSFHFGSMEH
                     RIIGMISHLGPQMALADGVALACKINREAACTLVFTGEGATSEGDFHEALNVAAVWQL
                     PVIFLIENNGYGLSTPIHEQYRCEKLVDRAIGYGMRGYRIDGNDLLEVHSTIRKLAEE
                     IRQHPEPILLECMTFRMRGHEEASGTKYVPKALLEEWEARDPLLRMEHKLLSEGVFSQ
                     AELDEIKLQGKQAIEAELAPVLEEAEIEAEEETEIRDVYRPVISRPLETQAGEKRAPT
                     RYIDAISQGLDQAMQLHPNLVLMGQDIAEYGGVFKITEGLLEKYGKDRVRNTPLCESA
                     ILGTALGLSLKGFKSMVEMQFADFVTCGFNQIVNNLAKQHYRWGQHADVVIRMPAGGG
                     TGAGPFHSQSNEAWFFHTPGLKVVYPSNPYDAKGLLLAAFEDPNPVLYFEHKALYRRA
                     TQEIPQGPYTLPIGKASLVRRGEAACIITYGMGVPEALQACEDHQWDIAVLDLRSLLP
                     LDYEAVEEVVRACHKVMILHEDSLTGGIGAEISAYISQYLFQYLDAPVIRVAGMDTPI
                     PFASNLEKQYLPWHRIESEMKNLLAY"
     gene            complement(21927..23006)
                     /locus_tag="PKM27_09770"
     CDS             complement(21927..23006)
                     /locus_tag="PKM27_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR07591.1"
                     /translation="MRPGFVIGLLLCCPSLWAQQQNLVPNPGFEIIIGQPERWFYTGS
                     DFNKVFNDWQSPTAASPDVYHEAIPVPAYWKEKGFFRLRPVSGKSMVGLTLYGCNQGK
                     LHCREYISVPLRDSLVVGQEYRLSLWLAPMKAGIPVDYIHAAFDREISHAMDDRLLEL
                     KPIYELKVDHTKTWQFLEIRFYAETEARFLTLGNFKDDANTQTGEGTVENGQPFGYYY
                     LDEISLTKIPPILDREPIESYNINDWNAGSSVTIENIYFDFDQSKLLPASFQELNKLL
                     LLLRQNPALRIRVCGHTDQVGSAAYNQSLSLRRAQAVADFLIRHYIEPDRLQVKGFSF
                     DQPIASNEDEAGRQRNRRVVFEILR"
     gene            23099..23815
                     /gene="pyrH"
                     /locus_tag="PKM27_09775"
     CDS             23099..23815
                     /gene="pyrH"
                     /locus_tag="PKM27_09775"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNR07592.1"
                     /translation="MPVKYKRVLLKLSGEALMGDQPFGIQSDRLQHYAREIKTAVDAG
                     VEVAVVIGGGNIYRGLSAEKSGVERVQGDYMGMLATVINGMALQSTMEDIGLYTRLVS
                     AISMAEIAEPYIRRRAIRHLEKRRVVIFSGGTGNPYFTTDSAAALRGNEINAEVLLKG
                     TKVDGIYTSDPEKDPDAVRYSRITFAEVMQKSLAVMDMTAFTICHENKLPIIVFDITT
                     PGHLLRILQGEESLGTLVTH"
     gene            complement(23919..25331)
                     /locus_tag="PKM27_09780"
     CDS             complement(23919..25331)
                     /locus_tag="PKM27_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR07593.1"
                     /translation="MENKYQHDHNLTQWVNEFEILSQQGEKTAFDLRIYQKLIDYYKQ
                     EGDLKKATEVAESAIEQYGYRVEFYLIKSRLLLSQREPLEAMDSLLQAERLSPRDTEV
                     LMTKALILAELRDTRAALSLLQRMKSYVGQTDLIEVLICETEIYFRAEENERAFITLK
                     EALLANPYHEKGQQLLSDYISVSRRYLPVSRFLQEMLEEAPYSHFAWYNLGHCYSNLG
                     EYDKAIDALEYAFLIRPEFEQAYTDCAEICMMKQQWDKALSIYQEVLTSFGEEYDLLV
                     SLAECCLHLKNFVQAKKYLSQSLHADPYGEEAHFLTGQCYAQEGKWHKAIQFYRRAIA
                     IDETSDTYLFALAKAYAKVGEMDKANKHFRKATTCAPEQNAYWFEYAAFLLNQGKLQQ
                     ALEVLDQGEISSVGSDLLYCRAAVHYLIGDKTKAIDILAEVLEDDFESHPCIFGLVPG
                     MGEDRDIMSIINYYRGEIFE"
     gene            complement(25778..26323)
                     /locus_tag="PKM27_09785"
     CDS             complement(25778..26323)
                     /locus_tag="PKM27_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNR07594.1"
                     /translation="MNRWMWNLIRKGFGWTIEGFDINSIPKVLIVVGPHTSGWDVLVG
                     LMTRGSLGCRIGFMGKHTLFKPPFGFIFRWLGGVPVDRTQKSNLVDIMASYFKSNNEF
                     RIAMSPEGTRQKVDRLKTGFYYIARKAEIPIIMTTLDYEHKRVVFSEPFYPSDDVEKE
                     LQRVWNYFKGINGYHPELSIQ"
     gene            26541..27086
                     /locus_tag="PKM27_09790"
     CDS             26541..27086
                     /locus_tag="PKM27_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNR07595.1"
                     /translation="MTDTEIVQLMLDERHQNTGLRQLMASYQERLYWVIRRIVESHED
                     TDDVLQNTFIKAFKNIGRFEARSSLFTWLYRIATNEALSFKKSRIRKAVQTLPESGMV
                     LAGGSASNGATEDQVLEKLDAALRTLPERQRQVFEMRYYDELSYEEMGQMLNLSEGAL
                     KASYHHAAKKIEQFLVETSTH"
     gene            27098..27601
                     /locus_tag="PKM27_09795"
     CDS             27098..27601
                     /locus_tag="PKM27_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07596.1"
                     /translation="MEESAKRKEIQDELRGLAPGLNKLPLTKNKEDLPFRYFEKLPDQ
                     VLRKLQEETLTARSKPGWLDRILQEWLPKKYMVLAMASLLTAILVIGLWPGSTPNLPE
                     ADFASVDRGEARSYLLTCAEDLDESHLALLVSQVEGDDFMPLSDEELDAVIEEYLYQQ
                     ASDMEMN"
     gene            27637..28056
                     /locus_tag="PKM27_09800"
     CDS             27637..28056
                     /locus_tag="PKM27_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07597.1"
                     /translation="MKTIRNFLLYSTLLIYSIHGYSQRGEKIEALRIAFITNKLSLTA
                     AESEKFWPVYNIYRREVAELRRQSFIDTNLEAMSDADAEKAIQVTIDRMEKELLLFKN
                     LARDLKPILPARKIALLSKVERAFNEEIIRRSQMGRN"
     gene            28184..30319
                     /gene="ligA"
                     /locus_tag="PKM27_09805"
     CDS             28184..30319
                     /gene="ligA"
                     /locus_tag="PKM27_09805"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNR07598.1"
                     /translation="MSKSDQLDQNIRWTTELMEMSPDQLLKQGPEHYDQLKSVIRFHE
                     HRYYILNDPLISDYEYDQLYKKLEFLEEKHPEWISRDSPTQRVSTDLTQAFETVDHLK
                     PMLSLANSYDEKDLLDFDKQVKKLAQLPATDKVTYCVEPKYDGGSISLVYENDQLVRA
                     ATRGNGFQGELITANARTIKTIPLKVPFKKDGIARAELRGEALIRKDTFRKINKQREE
                     EGLVLFANPRNAATGGLRTKDASETAKRGIEAVIYQVGYLEDEKGREINPYKDHAGGI
                     HALAAMGFKVPKVEFKVCHGIHEVIEFCHAWQERRDDFYFEIDGMVVKVNDLGLQASL
                     GYTSHHPRWAIAFKFKAKQATSRLIGVEYQVGKVGTITPVAKLEPVQVGGVTVSSVSL
                     HNEDFINAKDLHIGDMVIVERSGDVIPYIVKALPEYRKGYEKKIKFPVYCPIGAEFHV
                     KLVREPGQAFWICPDCKCGAQNLEKYIFHVSKAAMDIEGFGPSIMEKFVQLGWIKSLA
                     DIYRLDFEQIEKLEGFGEKSAGKLKSAIEKAKSNPIQRLLHSLSIHHLGKKASSLIAE
                     RVGYVPDLANWTENDFTNIPEIGPVVTKNVMDWFGNPKNLEVLKEMEALGVNMYQTRE
                     DKPKKVDAHAPLAGKTILFTGSLSQLSREEAEKKAEDAGARNLSGVSSKLNILVVGEN
                     AGSKLEKARSLGTVEIWTEDEFIQKVAET"
     gene            30334..31332
                     /locus_tag="PKM27_09810"
     CDS             30334..31332
                     /locus_tag="PKM27_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR07599.1"
                     /translation="MKNLSLLFLFLEAILIFPNLNCRRYKPLKMETDYTKEGMIPVQG
                     TSLHYLQWGEGGKALVILHGLGDSPYIFSHLAREWSSRFKVNAFARRGHGRSGSPDSG
                     YDNQMLTNDLKSLVDHLQLDRVFLLGWSMGGNEITEFAIRYPERTAGLIYLEAGYDYS
                     EPAFASILDQFPEALSPGPSDLVSLDAYRQWYHRFWYPDLDWNEVLESNLKACTRVLE
                     NGAVEIIPGEKITNLLVRSLTAYPRDYSKITAPVLALYTPVFFKHHSADPNLQKAYED
                     LENKIISPWRESNKEKLKKALKEVHIHELSMGGHASVLFASQDSVLKLVNDFVAAH"
     gene            31371..32321
                     /locus_tag="PKM27_09815"
     CDS             31371..32321
                     /locus_tag="PKM27_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HNR07600.1"
                     /translation="MEKIKIATAQFEHRSGDKAYNLGVIEKLAYQARDQGASAIAFHE
                     CSVTGYTFARRLDREQMLDLAEFIPDGPSIRQLTAIARDTGVTLLAGLFEKDRDKNLY
                     KAYVCVNEQGLVAKHRKLHPFINPWLKPGDSYCVFKLHGWTCGVLICYDNNIVENVRA
                     TALLGAQVLFMPHVTMCTPSPRPGAGFVDPALWHNREADPTSLRLEFDGMKGRAWLMK
                     WLPARAYDNGIYVVFSNPIGMDDDQLKNGCSMILDPFADILAECRSFEDSLVTATLVS
                     EKLGQSGGSRYIKARRPELYRDILGQEHQGEQKVAWMSGD"
BASE COUNT         8730 a         7520 c         7762 g         8596 t
ORIGIN      
        1 ataattacta aaattttttt gtaactatgt ctgaaaattt agggggctgt ttcagaaacc
       61 atagtcaaca ataaaattat aaaccccagt attgcacctt caaacttacc agttacagaa
      121 gagccggaaa ttaatgtagc attaaaatct gtaatgtttg ggtctatact ttatgatgcc
      181 gtaaataaag tgtactacag gttcgtttta aaatctcgta ctcaagatgc attaattcgt
      241 cctctctatt taaatatctt taacaaaccc atcaccattc aggtattgaa cgacaaactg
      301 gaagaaatgg gaacggtgga cattaaaagt aaatatatat atacaaagtc tcaggttcaa
      361 gatggacatc tgttccttat cagaaactgg attaataccg gagaagatca tttaatcatt
      421 gatgaaattg aaatcattct atagttattt tttcaaaaat cactacatga ttaatatttt
      481 aaagtctact tttatttatt tggagttctg aaatgttaat ttggaaatcg tcctcagggt
      541 gtatgtttaa atatctaatt ttaataggat gtttgaatgt ttcctgtaag gacccggaaa
      601 ggatgggaac ctttaagtta aaaggacaac ggggacagca tattaccaaa gttcattttg
      661 acaccataaa gttcaaaatt cccttgttaa aatttgggaa tatgaacgca ttaaaaatcc
      721 caaataccgc catattcggc ggaagggaag cattttctat atccaattac gctgaccaat
      781 ccatccatat ttttgatttt agagccagga atcacctgct tgccattcag ctttatacag
      841 agggtccgaa cactacgata agtgggaaat acgggtttta tcatcaatta ttgcctgatc
      901 aaaccgtttt aatcctggat cctcctacaa aaaggctcag cagggtagat atccggggaa
      961 cccgcttaga gtcccatacc ataccgccgg atccggattt tgaatatctg gcggtagtgg
     1021 attggtcgat ccagggagta ctgtatggcg acgatttttt ttatcccgta atcccaggcg
     1081 gatttatgcc ccttacgata caggatcccc aatacaaacg gctcaaacat ttgttacaat
     1141 accgtgtagg cgaaggattc cggtcgatga tcataggccg caacaacttt tatactcatg
     1201 aacgatttta caacttcgat gagtgctgca tgaccaattt tcttagcttc ttaaagaacg
     1261 atctgctcat ttccgattgc gtaaacccat ttgttcggat atatgaatat ggcacgggcc
     1321 ggcttaaaga ttccatagag atgaagtccc gggagtttga ccaagtgccc gcctacgatc
     1381 aacctgttac ggccggtgaa tatcagcaca tgccggccaa tttatacagg gaaattttgg
     1441 aattcgggca tacccaccct tcctattacc agttgcacgt ggataccact catcatctga
     1501 tcatccggca ggccaaactg ggcatgactg atgaagaata tatggacaac cggttctatt
     1561 ggtcgcattc ttttatcctt tcggacacgg accattttca atatcgcgga gaagcgatcg
     1621 ttaaaagagg cgattataag aatatatcgt tcctcaatgg cgctatgacc tccgaaggat
     1681 tatatttccc gttttttgat ccacagggta tggaggaaga tcatttgtat ttcctgaggg
     1741 cccggtttat tgaataagac cgccagtcac ccaggaacgc cagcgaagat atgattcagc
     1801 catacttttt ttgtggatcc gtatcaaaat ggaaatataa cttgttttaa atttatacct
     1861 tacccatttc atccaactaa ccatcgttcc atgaagcata taatatcttt tgtattgagt
     1921 tgttatagtt tctatatttg caatggtcag attaataatt ttaatattaa ttcattaaca
     1981 agggacccta cgactttaga ttcgattagt tttagcgccg gggctatttc agaatacgtt
     2041 gaatcaaatt attttttgtt ttctgacagc gctttaattg acggaccaaa tataaaaatc
     2101 aggtccaatg aaatgaaagt atattattta aaagaagatc atcctcgcca aaaatttttt
     2161 accgaagctc atatttgtgg tatagcttac ttttttataa ataaggagca aataaatttg
     2221 cctataggtt ataagaagtg cattttggat ttatcaaatt taaattatat ttttctgttt
     2281 gaatagaagc cttttaacag gttcaattga acctgattcc gctaattttc ctttggcttt
     2341 tcgtgcatgt cctaaacggt tatttttact caccctattt tacttcccca gttgctcaga
     2401 cctggtcgct ttggtgtctg ccgcattggt aattttgcct gaagggatcc tttacatcaa
     2461 ttcataaatt tccccttcat ccttatccaa taccagtaag ccagcgggat aaaccaggtg
     2521 gtgaaaaaag tcccgatggt cagaccccct atgatgaccc agaccagggg tttttgtaat
     2581 tcgtttccca gaccggggat gaataaaaca ggcaccaggg cgagagaggt ggtcagggag
     2641 accatgagca aaggcttcag gcagatgccg ccggcttcgt ggatcatccg ggacagggtt
     2701 tcttcattca atggaagccc ctgggactta taggctttgg ataagcggtt gagggtctcg
     2761 attttcagga tgggatcgtc tacgatcaat cccaggatga caataaaacc gatagccgcc
     2821 atcgtatcca gcgaacttcc agtggcccag aggagcaaca tggatccggt aatgcccagg
     2881 gggatggtga gcatcaccag gatgggctgt accaggcttt cgtattgaat agcgagtatg
     2941 acatacatca gcaggaccac gagcaggaag attaagacca actgccctaa ttgtttctgg
     3001 tcttcaaaat acctcccgga gaagtgtacc gggataccct ggtcaagggc aaagaccgac
     3061 agccgcccct tcaactcctc aaccgtagaa ccggttccgt ccggccatat taaattatgg
     3121 tattgcccgc tgcgatcagc caggatctgt tttggctggc ttcgataacg gaattgcaca
     3181 aaaagggaca gggggtaaga ctggccgttg gaggaacgca gccatggtgc ctggttttcg
     3241 tttcgttctg aaaccaggtt caccctgacc cggtccgacg cgttttgcag ttcggtcagt
     3301 ttataagagc catagtgttg ttcaagaacc tggtccaggg tctgccggtc gatcccgtat
     3361 aaagccatct tcggatagtc cggcaggatt tccaggtggg tctcagaggc caggccttct
     3421 cccggcaagg ccccagtatt tgccattttc tccgccaggc ccgatatcaa tgaatcctgg
     3481 ccgcgcgtga cattggtaaa cctggcttcg aaataaggca ctgatgagct gaataactgg
     3541 gtaaatgcgt tgggcgcttc ttccagttcc aggcttgcag cgggaaaatg tgtacggacc
     3601 caggcgaggg ccgcctcatc ggccttcgag cggtcggcat ccgaactgca tgcataataa
     3661 agcgatccct gctgcaccgt ttgacggcta tcctgaagga ggtactgggt aatcccgata
     3721 tccgcttcgg aggcaaggat aaacggacgg atttgctccg tgagcaggcc atgccgccgg
     3781 cggttttccc ccgcatcaat gggctcattc cagtcgatcc ggatcaggct ctccgtcttt
     3841 tgagtttccg gcaaggccct gaccggcagg aacctggcga tccagaatcc ggccggcatc
     3901 atcagcaggg tcaatacaaa ataaataggc cggtagcgga ggatatgacg gatcatgcgg
     3961 tggtacccct tttcgaccca gcggtaaaaa accgtgtctt cacggatctc tccccggctc
     4021 agccggctga gaagtacttt atagagcagg ggggccaggc caaaggcgat caggagggat
     4081 acgaaaaggg aaatagtcag ggctttggcc tggtcaccga tcaggtcacc ggccatgcca
     4141 tggagcaaga ccaggggcgc gtacaccact accgtagtca atacctggct gatcaccggg
     4201 ggcgccatct cgaccgtgcc gctgaccgcg ctttcttcca ggctcaatcc cgatttcctt
     4261 tttcttgaaa tgtggtcaat gaccacaatg ctgttgtcga tcagcatgcc gattcccagg
     4321 gcaagcccgg acagcgaaat gatattaaaa gacagcccga ataaatagaa aaaaaccact
     4381 ccgatcacca gggacagggg gatgctgatg ctcatcaggc tgggcgccga ccaatgtccg
     4441 agaaataaaa acagcagggc aatggtcagg atgcccccat agaggatatc ctgttttaaa
     4501 ttgtcaatgc cggcgaccag caaaaagctt tggtcctggc tgatttcaaa acgtacgccc
     4561 ggatagtctt tccgcaaaag gcctatggca tcgtaaagcc ggggcatcag ttcgtgcata
     4621 cgggctccgg cctgtttttt gatggtcagc acaagtcctt cttcatcctg gtatacatgg
     4681 tatccaagcc tgggttcggg gaccaggtct acccggcaga gccggtcgag cggaatggag
     4741 ccctggcgtg cctgcaccgg aattttccgg atcgtttccg ggtcatgcag ggtctgggcc
     4801 agcctcacaa aataccggta ctggccatcc cggatactca ggcttcccaa ttcctggttg
     4861 gcgttccgga tggcagtccc gatgtcggca gtttgcagcc cggcagcctg cagggcttgc
     4921 tggtcgggct gcacgacgat gatattttct acctggccat tgatatcgac ggtgccgacc
     4981 ccttcaagtt gttccaggcg ctttttcaat attttttcag ccagatcggt cagtgtggcc
     5041 agctcaggac cggaggaatc cggcaggaca tgaagccgga cgacggggat gtccgaagta
     5101 ttgacccggt ttacctgggg cctgggcaga tcgcgtggca gggattggct gaggcggtct
     5161 attttctcat tgacctccac ataagccagg tccatgcggg tgccgtgctc aaactggagg
     5221 tacaggcttc cgcgatgatt ggaagccctg gattgaatat ccaccaggtg gctggtagtc
     5281 aatagacctt cccggaggga ccggagggcg gaggattcta cctggctggc cgtatgcccg
     5341 gggtagctga gggtaatgat gatgcggggt acatcgatcg ggggcagcaa ggagacaggg
     5401 atggacctga aataataaag gcccagcacc agcagaaggc caaaacttaa aaatacggct
     5461 acagggcgtc ggataagata gcgtatcaat agtaggattt tataggatga taggattgtt
     5521 aggatttata ggatttatag gatttatagg attataggat tgttaggatt tataggatta
     5581 caggattgta ggatttatag gattgtagga ttgtaggatt gttagggtga tagggttaaa
     5641 ggatttacaa taaaattacc ttttcaacat tgaatgcgga gggaaatggc cttaaaaatg
     5701 gaaaattttt ataactttaa accatatatt ggagataccc cctacccaat gtagccctga
     5761 aatggcgtac tatggaacac gaaaaatttt actctaagca tgacctggct tttgagttgg
     5821 gcatatctct gaaaacgatg ggccagcgta tcaaaaaact ggaaaacggg gtggtgaaag
     5881 agctttacag ggagaagcgt cttttttcac cggccgaacg caaaattatt ctgaaaagcc
     5941 tcctggatca tgtaccctct aaccggtaaa tatggaccct tccagttatt tccagttatt
     6001 tccagttatt tccagttatt tccagttaat acttttctat tccaatgcag ttcaggaaat
     6061 ttggatcact tatcccccct gttttgcgat ggacgagacc ggttttccga aaacggacca
     6121 gagtaggata tttaatgtta attttttgtc ttatgaacag gctaattctt ttgattggat
     6181 ttaccatttc aattttatct attgcaaatg cagatgcacc ctgtcctaca ggagtgacga
     6241 ccgtccagac atcatggtgg ccaccaaata acgatacatg gttcaccttg tgtggttgcc
     6301 aggctgttaa aggatattcg gcttcaggat cgtgtggagg gccggaatag tcaaaaactc
     6361 ggggatagcc tttgatatcc tatatggata ttaaaagcaa tgcgacctct tttgggagta
     6421 tttactttag aggattttgt gcaaaatgac ataaagtgaa tatatatgaa aacaatcttt
     6481 ccggttttta ctttatctta attttgaata accagataca aataaatatt aacaggataa
     6541 tcatactatt tttttatttg gcatgggcta tctcctgctc aagggaagct aattcatccg
     6601 cggtgcttat cgaagatggc gtttcctcat ccctgatcat attggatata ccactcacgg
     6661 gtctggaatt gcccctgaag tccggaaaaa ttcaaagtat taatatttat aaggataagc
     6721 tcattattaa ggagagtaat gggagccata tccttaccgt ttattctttg aaggagggta
     6781 cctatacagg atatggcagc attggagaag ggccgggcaa aattcccaac cctgaattct
     6841 gcaagctggt gcatgtagag gagggaggat cggtgatgat ctttgatttc ttaacgaaga
     6901 agatttacag gctctctttg atggacaata cgctggcgcc gctttatact ttaagcaggg
     6961 aagagggtgc ggcccaatcc ggcatctggc taggggattc catccttgcc ctggcagatc
     7021 ctgccgggga cccgatccgc atcctggact taaaatcaga taaatccatc ccggcaagcc
     7081 tggaattgcc cttccggcgg acgctgactc cggaagaaag gatgcaatgg tatccgaaag
     7141 atcttgcctt taatactaag cataggctat tgttgacctg gagcatcgtg tcaaacacat
     7201 tgagcgtcaa tagcacgaac ggatataatt tgaaggacta ccagtttggc gccaatcaga
     7261 atctcgatga caaccaaatt catcggaata aaatatatta ttacgatgga aaaacatccg
     7321 gggattgggt atatgccctt tatggaggat ttgacttaaa cggtcagttc ctgaataaaa
     7381 taatttccca tatccggtct gaattgcatt tatatgatct taaaggggat acgcttcgcc
     7441 gcttcaggct tgaccggctg gtcaatacct gtgccatcga ttttacaggg aattgcatct
     7501 attgtatcca ggaaggcgga aaggaagata aaccattggt aaaatatgaa atggattaag
     7561 aggattggtt taattctcct ttcgggcctc ctggtcctgt ttttaccatt tttcgttgaa
     7621 tacgactttg gcttaaaaaa aagcccggga cccgaatgta agatttccca ggggatgaaa
     7681 caggtgtatg tccattggtt aaaacccctg aaccatacgg tttccgtatt ttgtgatgga
     7741 agccttagtc cacattatat gccctcaaag cggaacctgc catgcgattg caaacaagag
     7801 aaatgagttc gcttccatat tgttcaggaa tttattttgg atccgattcc aagctgtcat
     7861 tccagttatt tccagttatt tccagttatt tccagttata tattttgaca tgtgtttttc
     7921 ataatttaat tttgagatgc acttttagtt atacccaatt aaaaggtacg ggtacccgaa
     7981 aacggtctag agtagaggtt attattaatt tctttattat gaaaataaaa aaaacattaa
     8041 gcgttttggt cattctacta atggtattat tcggccaaca atttacggca aatgccagta
     8101 tttgcactta ctgcgcactt gaagggactc cgcaaggtat atgcaattgc gatccctttg
     8161 gcaatgatac ttgtgcatgg tgcgttcata ctggtggatg tgatgctaat tgttcatcca
     8221 cagaacagtg tgagcagtgg taaacaaata taatagacag gcattttata aaatttgcct
     8281 gtctaattta attctaaaat aatgaataaa tttattattt cggtattttt tttctccctt
     8341 ctgacctgca aactggataa aaaagagaaa ataaataatg atccaattaa aaaatatgaa
     8401 gtttcttttg atacattaaa atttctgtca cccgccatcc attattccag cgctagtctt
     8461 tttaaagtgg taaataatag cattttaaat gacattgaag taatgtccat ttccaatttt
     8521 attaataata ccatccagat ttatgatttt aaaaaaagaa agatctataa aaccattcaa
     8581 ttgcacaagg acggacctga ccgcaccctc accggacagt ttggatttac tcatcaaata
     8641 attcccggaa ataagattct tgttttagac cctttatcct atacatttag tgtgattgac
     8701 ctgaatggac acaagatcac atctcaaaga attccaccgg atccaaattt tgagtatgct
     8761 gccgccatgg aatattccat tcagggagta ttacatggag atgattttta ctatccagcc
     8821 attccggcac attatgcccc gctttccaat aaagataaat tatatgacaa gctaaaacat
     8881 ttgatacatt ataatacaaa tacgggattt cagttgccta agatcaatag aaacaattat
     8941 tacactttag atttaatata caataccgct caatgctgtt atgcgaattt tgtaacttct
     9001 gtaaacaata aaatttttgt ctctgattgc tatcagcccg atataaaatg ttatgatgcc
     9061 cggtcataca aatatttgta ttccattccg gcacattccg gttattttta ttcagctcct
     9121 tcatatgatg aacatctctc tgaacaggaa tatttatttc ccccagcaga aacttataga
     9181 tcaatggaaa ggtggggatt tgtgcatcct tcatattacc agatgtacat agatactata
     9241 aatcattcca taatacggca agcaaaactg ggcatgtctg atgaaccata ctatagagac
     9301 cggacacaat ataccagttc atttattttt atagactatg ataccaataa aataaaggga
     9361 gaggctctgg taaaaggggg tatgatagaa aaagtagctt tttttaacgg atttataacc
     9421 tcagaaggac tttgtttccc ttttattgat atccaacaaa atgaggatga tcatatctat
     9481 ctggtaaaag cggtatttca ttgaattgga tcattggatc tattttctcc ggagaggtca
     9541 cattaaatgg aattttgcca atagtggatc cggattttga atatctggcg gtccttgatt
     9601 ggtctattca gggagcattg catgatgacg aatttttata ttctgtgatc ccgggagctt
     9661 ttatgaccac ttaccaccaa aggacccaaa atttgggcga tttaaacatt gcatttattt
     9721 tgggcccggt gtattgaatg gagactgccg tcaccctgga acacagggat aattcagacg
     9781 aagatagcga ctcagccata actatatttt gttgctccgg gtcaaaatgg atttgtaact
     9841 tgtacggaga tgattaaggc tcatcttgct cggttgccgg aaagaaaggg ataccctctg
     9901 tttaagctat tgtcgatcac ttttatcgtt ctgttttcag aagcttgtcg ttacgaacaa
     9961 attccacaaa aattcagtat aagagtggat tccagcctgg ggacggatat cgcagacttg
    10021 tttttatccg tggaatattt gccgctgtcg aaatcgccga acccatttca cagcattgac
    10081 caactgagaa tatcggcaga ctggatctac ctattgatca gccaggcggg agagggtcag
    10141 tctgaagtga tgatctttgg gcgggatgac ggtcattatt ttcgttcaat aaagggagga
    10201 ttttcaggac cctatgatat aaggttcata agttgtattg atatttacaa gaaccggctc
    10261 tatgtcatgg atccgttaca atcgaggatt tttgtatatg acgaacaggg agtccgactt
    10321 accggattat ccgtcccgga tgagtatcgt aagttcgctg tattgaatga acatgtttta
    10381 atcctcgatt ccgatgaggc gccgacagcc tcggggacca ggctattgta ttttaacact
    10441 caaaatggaa agtccatgcc gctccgtcac aatggaaaag tccaaagcgg gatggcctgg
    10501 gaaagattct tccgcaaatc caattcagat cttttgtttt acacggccca cgatcccaac
    10561 gtataccaaa ttaaagagtc cggctcggat accctctatc gttttgattt tggcaccagg
    10621 ctgatcctgg aatcagatat ccctcccaat ataaatacca agctgacgat gttgaatgac
    10681 ttgatggatg aagggagaag attcaccacc atgtttagct ttatggattt taacagcacc
    10741 cttttcttca cgtattattt aaataaccat atgcgatttg tcagggttgc tatccgggac
    10801 agcggctatc ggacagatca gtttgagttt aaacgaaata ccctggatgg cggacctttg
    10861 cccgatctgc ctcctttggg atatactgat tttggagaaa tggtatttgt ttatgatgcg
    10921 gaatatgtat accacctgtg ccaggacaaa tccggaaata aaaggaagtc gcccgacctg
    10981 atcgcattgt gcaataaaat aaagattgaa gataagccgg tgcttgcgtt ttatagaatg
    11041 aaataatggg tttatgaagt acttaatatt atttgttttg aattttttta gtttcctgaa
    11101 ttgcaatgat ccagttcaaa aaataaaaaa agtcacttta attaattttt tatttttttc
    11161 attattcatg gtggctatat taacgctgtt tggctgtaaa aaacctttaa ataatgaggt
    11221 tatagaagtt aaaacaataa ataatttccg tattgatacg atattcctat tagcagcaaa
    11281 ctcaaaattc gtgaatccga gaacatacaa aatagttaat aataattatt ttatggacac
    11341 agaagttact tctattggaa acattgtaga taagaagctt tatatatttg attttaaaga
    11401 aaggaaatta gttaagacga ttcaacttta ttctgaaggt ccaaatacca caatttatgg
    11461 tggttataac gtttatcatc acattattaa tgataacacg ataataatta tcgatgctcc
    11521 atccggtaaa ataagcagaa tcaattcaga aggcataaaa cttaaatctt ttgatattcc
    11581 agttgttgga aacaatagtt atggtttatt tattgatttt atgattcaag gggtttacgc
    11641 aaataattta tttttatatc cagtagctcc ctggggtgta tttccagaaa cagttgaaga
    11701 ccttaatgta ttggacctat ttcatttatt atccattgat ttcaataaaa accacgtaaa
    11761 ttttaagatt aaaaggtcga acctgtatac attagataaa ttatattatt tagaaaattg
    11821 ttgtcaaaca aattttattg ccaataaccg ggatgtagaa ttatttattt ccgattgttt
    11881 taatcctgaa atttcagttt attcgttaat cggtttaaac aaaaaaacaa actttattat
    11941 gaaatccaat gagttcaaaa acgttcctcc atattataaa aaatttacaa gttctgaatt
    12001 tagtaattta cctgcccaga gctttcggga aatattggaa ttcggccata aaaatccatc
    12061 ttatgctcaa ttgcatgtgg acacatttaa caatttgatc atacgacaag ccaaattggg
    12121 tatgagtgaa gaaaattatt atgaggatag gacaaaatgg gcccactctt taattttaca
    12181 tgatcttact acgcttaaat ttatcaatga attaaagata aacaaggatg aacatcttga
    12241 tattaacttt ttagacggtt ttatgacttc ggaaggcttg gtttttccat ttaaagtcat
    12301 tcaaagtaag gaaaatgagg atattttaca atttgcaaaa attacctttt taccggaata
    12361 attggcaagt aaattattac caagtacctt aaagcgtatt ttccttactc atacattacc
    12421 attgcttcat gggccagttg gaggttattg gttgtgatca cccggtctcc aggcttgagg
    12481 ccgctgagta tttctacaac gattccatta ttgcgccctg cctctacata attccatttt
    12541 gcctggttat tttccacagt aaataccacc tggcggtttc cacgtaccac cagggcctct
    12601 ttgggcacct ggagggattt tcgaacgggt attttaatga ccgccagtcc atgcatgccc
    12661 gggtatagtt tattgttcca gcttccctgg acttttacgg ccaccaggga catcccgttt
    12721 tggtccacct ggggattgat tctatgaacc attcctttta cggttgaacc aggatctgca
    12781 accggcaaga tatccgcgct ttggccaatc cggatttgag gaagatccgc ttcaagcacc
    12841 tggcattcga tcaggaggct gtggctgagc aaggtgaaca attcgtttcc ggcttgcaca
    12901 tattgaccgg ccaggtgttt agtatcagcc actttcccac tgaaaggggc aaggatcacg
    12961 gctttgcgga tggtctgttc caggtccttc agttccagtt ctgccaaagc caggccgctg
    13021 gaaatatgca gttttttccg gacagtcagt ttctccgctt catccatccc gaggaagagt
    13081 ttttcatagc ccaggagctg gctctcaaat tccttttccg ccagataccg tttgatttcg
    13141 agggcggccc tcctggatgc aaggtcaatg gtatccagtt gaaggatgac ttcaccggcc
    13201 tgaataactt cgccattggt cgctctggta ttgagtacgc ggccattggt ttcagctgtc
    13261 aactttaagg ctttttccgc tttaaagatc ccattggctt cgacccggta ttcaaaccat
    13321 ccttccgtgc atagcgccgt cctgaccgga gtggcggtgg tatccctcgt tgagtttacc
    13381 agaggaggct cggacggatc tgcaggggtc ttgcaggctc ccaaaaagtt taaaaaataa
    13441 agcaggataa agaggttacg gtgccgtata ttcataaggt taaaataata aaaatcaggg
    13501 attccaccgg gcctgccgac tggttaaata aaacggtaaa ccctgattaa attttggaaa
    13561 tgacggacac ctatggtcag ccctttcagg accgtattta tatttatcat ccttggaata
    13621 ctgggaggct tcagcattcc atatctcagc attgatttat ttccgaagga ggcttccgac
    13681 cggctgaaag tacaggtggc tttgccgggt catccccctg aactggtaga atccatggtc
    13741 acccaggcac ttgaaaacgc cttttcaggc cttcccggat taaaatcgct gaattcggta
    13801 tcctattatg accgggcgga catcgagctg atttataata aaggccggga tttggatttg
    13861 gcccggtttg aattggccgc catgctcagg agggtatttc caggcttgcc ggccggcgcc
    13921 tcttatcctg aaattttggc ccatggcggg aataccgatg ctgtcaacag atttgaagcg
    13981 ccgtttttga tctactccat tcgggcgccg gcccagcctt tttcgatccg gaacgaggca
    14041 gataaattat tcaggaagga atttgcaggc gtccccggcc tgaaagaggt aaagattacc
    14101 ggcacggaag accttcagct ggtcatccgg tatgatttga aacaatgcgg ggcctatgga
    14161 atcagtccgg gagagatttt gaacgcagta ggcaaacaat accgggtaag ttatccgggc
    14221 gtttataccg atcaggatgg gagcgcatat tttctcaagg tgggccgggc ggaaacaggc
    14281 ctgtccgggc ttgagaacct gatcctgccc gcaggtgatc aatccatcaa gctcagggat
    14341 attgcagagg tattcatcga agaacaacct gtgcagggac attaccgggt gaatgggcaa
    14401 aacagcgtca acctgatgtt ttttgcacag gaaggaagca accggatcct gctggcccga
    14461 gaaatccgtg aaaaaatggc ctccatggct gatcttctgc cttccgggta ccgggctgac
    14521 ctggtgaagg acgagaccgt atttctttcc gaagaattga ataaaaacta caaaaggagc
    14581 ctggccgccc tggcggtact gatcgttttt ttaatcctgg gttacagaag ctggaggcac
    14641 ctggtcatat tgatccttac cctggtcatc aacctggccc tgaccgcgct ggcgtgccgg
    14701 atcttgtcca tccctgtcca tttgtattcg attgccggcc tggccatatc ctttgggatc
    14761 atgattgaca acagcatcct taccctggac tattttcatc aataccggag ccggcacgta
    14821 ttcctcgccg tgttgggagc caccctcacc acggtcgcag ccctgttgtt tgtattcctg
    14881 cttcccgaag aaatgaaacg caacctcgtt gatttctcgg ccatggtaag catcgcgctg
    14941 gtcgtttcac tcgcagtatc cctttggttt accccgggac tctaccaatt gttctatccg
    15001 gaaagccggc aggcagaccg gaggtccggc atggagggca ccggtttgtc cggggtgacc
    15061 ttattttatt tcaacagtat tcgcttgttg gccaggttca ggaaaacctt cctcgtatcg
    15121 gtggtattga tgtttgggct tcctttgttt atgctgcctt cttcctggcc gggtaaggaa
    15181 tggtataata aaacgatagg gaacacatgg taccaggaac acctccggcc tactatcgac
    15241 cggatcaccg gaggggccct ccggttgttt gtccgcgaag tatacgaaag gtcagggtac
    15301 cgggatcccc aaaggacccg gttatatatc agcgcccagc ttggcccagg aaacaccctt
    15361 gaccagttga atgtcatcct gagtcagttt gaaatatacc tgaaagaggt ggagggcatt
    15421 gatcgttatg tgacctccat aatttccgga caatatggct ttatagaaat cagtttctta
    15481 ccggaatttg aacacggcat cctgccctgg cggctgaaga gccagttgac ctccaaagcc
    15541 acggactgga gtgcggtgga gtggggaatt tttggggtag gccagggttt cagcaccatg
    15601 cggcaggatc agatcccgtt attccgggta ttgatgaaag gttataacaa cagggaattg
    15661 gagcatcagg cccagctcct gaaggagcgc ttgcttgccc acccgagaat tcaaaaagtg
    15721 aacaccaatg aacggttaaa ctggaatgag aaagccagcc tggaatacct cctgaccctg
    15781 gatgagcagg ctatggcggc cgggcgaatg gataaacaaa gcttgtttag ctccttgcga
    15841 aacctcagtc agcccatatc taccggccag gtgattgagc ttggagacaa gtcctatccc
    15901 ctggtcatgc gtgaagccgg cacgcaggag tatgatctct gggatctgaa aaacaaggcc
    15961 ctgcaggggg acacagccag ggcttttaag cttggaaaat tgtccgggct ggcactggtg
    16021 cccactgcca gtgcggtgca caaagaagac aggcagtata tccggctggt cgcttttgaa
    16081 tacctgggca gcgagtattt cggatcaaaa tatcttgacc aggtattggt cgaaatgaaa
    16141 tcacttcttc ctccgggtta cgaggccagc aggaaacggg gacgatgggg gtacggcagc
    16201 caggctaaac cttatggaat tctcctgctc ataatcctgg ccaatttttt tatttgtgct
    16261 ttattgttcg agcacctggt cttgccgttt tatatcatcc tgatgatccc cgtttccttc
    16321 accggtttgt tcctgacctt tgcctggggt gatttttatt ttgaccaggg aggttatgcg
    16381 gcttttgtga tgctgaccgg gctcgtggtg aatgcagcca tatttattgt acacgactat
    16441 caacaggctg taaaacaaaa tccgggactg cctggcaact cattgttgat tctcgtaata
    16501 gccaaccggt cgcgcaccat tctgcttacg accattgcgg ctgccagcag tttgttgcct
    16561 tttatctggg aaggtcagaa tgaggtattc tggttttcat tggctgttgg ggtcctgggt
    16621 ggattgagct tttctatgtt tgccgttttt atcgcgcttc cggtattgat ctggaaccat
    16681 tcagaaagtt ttccaaaata aattcccaca ttgactgatt ctttttatgc ctattgaaga
    16741 attggagagt aatttatgtt aatccagtta tttccagtta tttccagtta tttccagtta
    16801 ataatttaaa ggcaacagtc aaatgactaa atttattaca tgttcttaga tacactacaa
    16861 tctggcgcca ggaactttca agttttgaga acaaagggtc aataaaacaa aacatactgc
    16921 aatggtattg ttttatagaa ctttcataat gattactacc agtgtatgga tgtgcacgtt
    16981 tttttcatgc aaatggggtg accaagggta tagaaatgaa gaaaactgtc atatggatac
    17041 gggttctacg atcaatatag atgtcaagga cgccctgaat tcatataaaa catcgtccac
    17101 gttcttaaat ttggttgtcg cttataatgg catgaatcat tccttagatc tgtttaacat
    17161 tagtgataaa tcttttatcg cttctcataa gcttgagtcc gacgggccct ttggattagg
    17221 tattatacga aagcttcatt tttattcaac agacagcatt tttatatttt ctgaaaataa
    17281 agtttttatc tgcgatactt cgtttgatca ctgctctgcc atacctcttc ccggggaaac
    17341 aaaccctgtt tccggcttca accctgaaag gtattatata cactgggggc ttcgtggggt
    17401 caatcctttt tattttaaag gatctgttta ccttccctgc ataaagctgg atgaaaaaac
    17461 cgaagccggc ttaaaaccac tctgctgcaa atacgacatt ccttccggct cagctgaaat
    17521 gatagatatc ccggaagaaa taaaaggccc ggacacctgg tatggcatcc ttgaccaggt
    17581 aaattattct ctctttgact cctttttgta tttcggattt cgttattctc cggatatata
    17641 ccgttatgat ttaaattcca aaatatttca accggtttat ttaagcgata tcggggagaa
    17701 tccgccgtcg aaaagagacg gatccatgga ttatcccgta tttaatatca tgaattatta
    17761 tacttatcat gaattaaaga tcacaccagg cggcaccctg caggcaatat ttaataaacc
    17821 cgggaaggaa agtcccaaaa ccggccgaaa agtcattaat gaaatgttcc tccgggccat
    17881 tcatttggcg gattcgcata aatcagcaga agaactatta atcctttcga aattagatcc
    17941 attcaaaggc attctatata acaatgccat atattttcct ttagataagc ccgactccga
    18001 gaacctgatg aggctgcacg gttatgagtt ggtatgcaaa tagtgattgc aatcgaatgg
    18061 ccttatttaa ttaaattaat tctttaatta ttaattcctc acaagcattt cctccaattc
    18121 cttaaggata tcctccatcc tgccgctcga tgtattattt aacaggatca ccgtatattt
    18181 tttatcaata taccttacta tatgggtccg gtatccggga ttatccccgc tgtggtgaac
    18241 cacccttccg agggtaggat gggtcctgag cgcccagcca aaaccataat tggagatgcc
    18301 tccatccgat aacctatagg gttgaaatgc ttcttccagg attttctgac tcaccggttt
    18361 tccggtgtac agagcccggt cccatttcaa caggtccagg gcggatccgc tgatccttcc
    18421 cggccccatg cgttcgccca gccaaatggt atagttgaag gcaggaaagg aatccgcgtg
    18481 ggtatagcgt tttttatctt cttcatagat atgaccccag gcaaagtttt tgatccggct
    18541 tcggctgccc ggattgcgga agcccgcccc gtgcattttc aaaggatcaa agatccgttc
    18601 attgacccac tctgtgaaac tgcggccgga gacggcttcg gcgatgctgg ccaacagcag
    18661 gtaacccgta ttgctgtaag catattttga accgggcggg aatagcatgg gcctggggta
    18721 ttgtttgaga taacggatca cctcctcatt tcctgcaatc ctggatttgt cccagtattg
    18781 gtccatgatc tcctggtaat ccgggatccc gctggtatgc gtgagcaaat gccggatggt
    18841 gatgccgtga taaggaagcc cgggaataaa ttttccgagg ctgtcttcgt agttcagttt
    18901 gccgacttct ttcagttgca ggatcaccat cgaagtgaag gatttggata tggaagccca
    18961 ttcaaacaga tcggttttcc ggagtttatg ttccccatta aagcgacggt agccttttgc
    19021 cttgtaatac accggcttac cttccctggc gaccagcagg accccgctga agtcgaactg
    19081 gtcccattcc cgctgcatca attttcctat ctcctccctg agaagggatt gcgcccggac
    19141 aggcaggctc attatacaaa gcaggaagat caggatacgc atcagaatag aattttaaac
    19201 gcaatccatt gggatacaaa tccggccaaa taacccaggt agatttcacg cagggtatgg
    19261 gcgcggagaa acatcctgca ggtacccacc aggccggcaa gcagcaaaac ccaaaccagg
    19321 gcggagtacc agctcaaaga ggcaaggcct tctcccggga gatccacggt aaacgtttca
    19381 tgactgaaaa aattaaaggt aagccaggtc atggccacca cccctcccat gaccgaggta
    19441 tgggcgctga tcttgaagaa aaggttgctg acgaacacca gcccgagggt aatcagcgct
    19501 ccgaaaataa aaatattgta aaccagggga acggcggcgt catgacggat actcaggtac
    19561 aaccacaggt aaaagatcat ggtcaccata taaggcccga tccggtcgag gcgttcagtc
    19621 aactgatagc tgttgatcag ggccagtttt ctcatcagaa aaatgctgaa aacgggtaaa
    19681 acgaaggtca gggcaaaaac ctggagcaga aggctggtcc gggcagaggc ttcgttgacc
    19741 ccaaataaaa aaggattaag caccagcagg aggtacaatc ccaggcttgg ccaccacagg
    19801 ggatgaaaga cgacggatac aagtatggcc agccagcgcc agggggtgat gggtatgtta
    19861 aatagccgca ctgccattaa taagccagta aatttttcat ctcggattcg attcgatgcc
    19921 agggaaggta ttgtttttca agattcgaag caaaagggat gggggtatcc ataccggcca
    19981 ccctgatgac cggggcatcc aggtattgaa agaggtattg ggaaatatag gcgctgattt
    20041 cagcaccgat ccctccggta aggctgtctt catgcaggat catgacttta tggcaagccc
    20101 tgaccacctc ttcgaccgct tcgtaatcca gggggagcag gctgcgcaga tcaagcaccg
    20161 cgatatccca ttggtggtct tcacaggctt gcagggcttc cgggaccccc atgccgtagg
    20221 tgatgatgca ggcggcctcg ccgcgccgca ccaggctggc tttgccaatg ggcagcgtat
    20281 aagggccctg gggtatttcc tgtgtggccc gcctgtataa ggctttatgt tcaaaataaa
    20341 gcaccgggtt tgggtcttca aaagcggcca gcagcagtcc tttagcatcg tatggattgg
    20401 atggatagac cactttgagg ccgggcgtat ggaaaaacca ggcttcgttg ctctgggagt
    20461 gaaaggggcc ggcgccggta cctcctccgg ccggcatccg gatgaccaca tccgcatgct
    20521 ggccccaacg gtaatgttgt ttggccaggt tgttgacgat ctggttaaaa ccgcaggtga
    20581 caaaatcggc gaattgcatc tcgaccatgc tcttaaaccc tttcagcgaa aggccgagtg
    20641 ctgtgccaag gatcgcgctc tcgcacaaag gggtattcct cacccggtct ttgccgtatt
    20701 tttccaggag gccttccgtg attttaaaga ccccgccgta ttcagcaata tcctgcccca
    20761 taaggaccag gttggggtgt aactgcatgg cctggtccag gccttgagaa atggcatcga
    20821 tatacctggt gggggcccgc ttttctccgg cttgcgtttc caggggcctg gagatcaccg
    20881 gtcggtagac atcccggatt tcggtttcct cttccgcttc gatttcagct tcttccaata
    20941 cgggggccag ttctgcctcg atcgcctgtt ttccctgcag tttgatttca tcaagttctg
    21001 cctgtgaaaa aacaccttca ctaagcagtt tatgttccat gcgcagcaag gggtccctgg
    21061 cttcccattc ttcaagcagg gctttgggta catatttggt tccggaggct tcttcatggc
    21121 cgcgcatccg gaaagtcata cattcgagaa ggatgggttc ggggtgttgt cggatctctt
    21181 cggccagctt ccggatggtc gaatggactt ccagaaggtc gttgccatcg atgcgataac
    21241 ccctcatgcc gtatccgatg gcccggtcca ccagtttttc acaacggtat tgttcgtgga
    21301 tgggagtgga taggccatag ccattgtttt cgattaaaaa gatgaccgga agttgccaga
    21361 ccgcggctac attgagggct tcgtggaaat ccccttcact ggtggcgcct tccccggtaa
    21421 acaccagggt gcaggccgct tcacgattga ttttacaggc cagcgccacc ccgtcggcca
    21481 gggccatctg ggggccgagg tgggagatca tgccgatgat gcggtgctcc atgctgccga
    21541 aatgaaagga ccggtcacga cctttggtaa atccacccgc cttcccctgc cattggctga
    21601 acagccggcc caggggcacc ttgcgcgcag tgaaaacacc gaggttgcgg tgcatggtaa
    21661 aaataaattc ttcaggctgc agggccagcg cggcgcccac cgagatcgct tcctggccta
    21721 tgccggaaaa ccattttgaa atcctgccct ggcgcagcag gttcagcatt ttttcctcga
    21781 tctgccgggg cctcagcaat tccctgtaaa atcccttcag cacgtccatt gaaacggaaa
    21841 gataccgcaa aagcgcaatt gttttttcgg ggcagaaact tcgaaaggac ggcaagatcc
    21901 cagggcgatt ggggctccct tcccttttac ctgaggattt cgaataccac cctcctgttt
    21961 ctttgtcttc ccgcttcatc ttcattggaa gcgatgggct ggtcaaagct gaatcctttc
    22021 acctgaaggc ggtcgggttc gatatagtgc cggatcagaa aatctgcaac ggcctgagcc
    22081 cggcgaaggg aaagggattg attgtatgcc gccgatccga cctggtcggt atgaccgcaa
    22141 acccggatcc ttaaagcagg attctgacga agcaggagaa gaagtttatt gagttcctgg
    22201 aaagaggcgg gcaagagttt gctttggtca aaatcaaaat agatattttc gatcgtcacg
    22261 ctgctcccgg cgttccagtc gttgatatta taggattcga tcggttcccg gtcaaggatc
    22321 ggggggatct tggtgaggga aatttcgtca aggtaataat aaccgaaagg ctggccgttt
    22381 tcgactgttc cctccccggt ctgggtattg gcgtcgtctt taaaattccc cagggtcaga
    22441 aagcgggctt ccgtctccgc ataaaaacgt atttccagga attgccaggt cttggtatgg
    22501 tccaccttca gttcataaat cggtttgagc tcaagcagcc ggtcatccat ggcgtgggag
    22561 atctcgcggt caaaggccgc gtggatgtag tcaaccggga tcccggcttt catgggggca
    22621 agccaaaggc tcagccggta ttcctgccca acaaccagcg agtcccggag gggaacggag
    22681 atatattccc tgcaatgcag tttgccttga ttgcatccat aaagggtcag accgaccatg
    22741 gatttaccgg ataccggcct aagcctgaaa aaacctttct ccttccagta agcgggcacc
    22801 gggatggcct catggtagac atccggcgaa gcggccgtcg ggctttgcca gtcgttgaat
    22861 actttattga agtcgctgcc ggtataaaac catctttccg gctgaccaat gattatttca
    22921 aaaccgggat tgggcaccag gttttgttgc tgggcccaca gggatgggca gcacaaaagc
    22981 agaccaatga caaaaccggg gcgcatgagg acataagata aatgcttgca agttatccat
    23041 ttggcaagtt tatgtcaagc atttagtact tttgcgccgg aatctaaacc aagccatcat
    23101 gccggtgaaa tacaaaaggg ttttgttgaa attgagtggg gaagcgctga tgggtgacca
    23161 gccttttggt attcagtcgg accggttgca gcactatgca agagaaatta aaacagccgt
    23221 ggacgcgggg gtggaagtgg ccgtggtcat tggcggcgga aatatttacc ggggactaag
    23281 cgcggaaaaa tcaggagtgg agcgggtcca gggggactat atggggatgc tggccaccgt
    23341 gatcaacggc atggccttgc aaagcaccat ggaagacatc gggctgtaca cccgcctggt
    23401 ttcagccatt tccatggcgg aaatcgcaga gccctatata cgccgtcgcg ccatccgcca
    23461 cctggaaaaa agaagggtgg ttatattttc cggggggacg ggcaatcctt atttcacgac
    23521 ggattcagca gccgccctga gaggcaacga gatcaacgcg gaagtcctct taaaaggcac
    23581 taaagtggat ggaatttata cctcggaccc ggaaaaggac ccggacgccg tccggtattc
    23641 ccggatcacc ttcgcggaag tcatgcaaaa atccctggcc gtaatggata tgacggcctt
    23701 taccatctgt catgaaaaca aacttcccat catcgtcttc gacattacca ccccgggcca
    23761 tttgctgcgc attttacagg gagaagaaag cctgggcact ttagtgaccc attaattccc
    23821 ttaattcctt aattccttaa ttctcttaat ttttaattaa taaaaaaagc cggtgcattg
    23881 ctgcatccgg cctttctcat ttaaagtttt aaaatatatt actcgaaaat ttcgccgcgg
    23941 taataattga tgatcgacat gatgtcccgg tcttcaccca tgccgggcac caggccgaaa
    24001 atgcagggat gagattcaaa atcatcctcc agcacttccg cgaggatgtc gatggctttg
    24061 gttttatccc cgatcagata atggactgca gcccggcaat acaagaggtc acttccgacg
    24121 gaggatattt caccctggtc caggacttca agggcctgct ggagctttcc ctggttcaga
    24181 aggaaagcgg catattcaaa ccaataggca ttctgttccg gggcacaggt ggtggcttta
    24241 cggaaatgtt tattggcttt atccatttcc cccaccttgg cataggcctt ggccagtgca
    24301 aacaggtagg tatcgctggt ctcatcgatg gcaatggcgc ggcggtaaaa ttgaatggct
    24361 ttatgccact tgccttcctg tgcataacac tgtccggtga gaaaatgggc ctcttcacca
    24421 tatgggtccg catgaaggct ttgggacagg tatttttttg cctgcacgaa atttttcagg
    24481 tgaaggcaac attcggcaag ggacaccagc aggtcgtatt cctctccaaa ggaggtcagc
    24541 acttcctggt aaatggacag ggccttatcc cattgctgtt tcatcatgca gatctccgcg
    24601 caatccgtat aggcctgctc aaattcaggc cggatcagga aagcgtattc aagggcgtcg
    24661 atggctttat catattctcc cagattggag taacaatgcc ccaggttgta ccaggcaaaa
    24721 tgggaatagg gcgcttcttc aagcatttcc tgtaagaacc gggataccgg caggtaacgg
    24781 cgggataccg aaatatagtc cgataacaat tgctggcctt tttcgtggta gggatttgcc
    24841 aacaatgcct ctttcagcgt tataaaggcc cgttcatttt cttccgcccg gaaataaatt
    24901 tcagtctcac agatcaggac ctcaatcaga tcggtttggc cgacatagga cttcatccgc
    24961 tgaagcagac tgagggcggc ccgggtatcg cggagttcgg ccaggatcag ggccttggtc
    25021 atcagcactt cggtgtcccg gggactcagc ctttcggcct ggaggaggct gtccatagct
    25081 tccagcggtt cacgttgtga caaaaggagc ctggatttga tcaggtaaaa ttccacccgg
    25141 tatccgtact gttcgatggc ggactcggcc acctccgtcg cttttttcag atccccctcc
    25201 tgtttgtagt aatcaatcag cttttggtaa attctcagat caaaagcggt cttttcaccc
    25261 tgctgagaca atatttcaaa ctcattcacc cactgggtca gattatgatc atgctggtac
    25321 ttattttcca tgcaatggga ttatggagat aattgtaaaa gtgtttcagg gatgaaaaat
    25381 agagaaatca aacaatctgc aaaacttttt tgaaaaaaaa tttttcaaaa ctatctcata
    25441 atgaacacat tatgaggaaa atgaatgtta caagccacta ttatataagc cgttacatat
    25501 tgcccttaac aataatttaa tattaacccc ttaaaggtct aatactaaca catatttaat
    25561 ttcgtaagaa atgaggtgtg cactcaaatc cagacgtttc gggacacctt cagcacctgc
    25621 tgttccctga tccctgatcc ctgatcccag attaccgacc agtgactatt gactgtttac
    25681 tattgactat tgattgctga ctactgacta ctgactattg actattgact attgactatt
    25741 gactattgac tattgactcc tgactactga ttcctgacta ctgaatactc aactccggat
    25801 gatacccatt aatcccctta aaataattcc ataccctctg caattccttt tccacgtcat
    25861 cggagggata gaatggctcg ctgaatacca cccgcttatg ttcataatcc agggtagtca
    25921 tgatgatggg gatttccgcc ttgcgagcga tataataaaa accggttttt aatctgtcca
    25981 ccttttggcg ggtcccttcc ggcgacatgg cgatccggaa ttcgttattg cttttaaaat
    26041 aactggccat gatatccacc aggttggact tttgggtgcg gtcgacaggc accccaccca
    26101 gccagcggaa aataaaaccg aaggggggct tgaataaagt gtgttttccc ataaaaccga
    26161 tccggcatcc caggctgccc cgggtcatga gcccgaccag gacatcccaa cctgaggtat
    26221 gaggccccac cacaatcagg actttgggga tggaattaat atcaaatcct tctatggtcc
    26281 agccaaagcc tttcctgatc agattccaca tccaccggtt catacgatgg ttcaaattta
    26341 gccggaattt gcatgataaa agcaggggat gcgatcccgt ggtgcatcct gccttgaaac
    26401 ccggcaagcg gattcggcag gtacggagtg aatggctgtg ccgttagccg ccggctctat
    26461 tttacctaaa acgggcttta aacctttcct tatattaatc gtctattgga caggtccgat
    26521 aggaataaac agataaaatc atgacggata cagaaatcgt gcagttgatg ctggatgaaa
    26581 ggcaccaaaa cacgggtctg cgtcaactca tggcctccta tcaggagagg ttgtactggg
    26641 tgatccgccg gatcgtggaa agccacgaag ataccgacga tgtcctgcaa aacaccttta
    26701 tcaaggcgtt taagaacatc ggccggttcg aagcccggtc ttctttgttt acctggctgt
    26761 accggatagc cacgaatgag gccctcagct tcaaaaagag caggatacga aaagccgtac
    26821 agaccctgcc ggaatcggga atggtcctgg cgggcggctc ggcatccaac ggcgccacgg
    26881 aagaccaggt cctggaaaaa ctggatgcgg cgctgaggac cctgcccgaa cggcaacggc
    26941 aggtatttga aatgaggtat tacgatgaac tcagctacga ggagatggga cagatgctga
    27001 atctgtcgga gggagccctg aaagcttcct atcaccacgc ggcaaaaaaa atagaacaat
    27061 tcctggtgga aaccagtaca cactaataaa gaaaaaaatg gaagagtctg caaaacgcaa
    27121 agaaatacag gatgagcttc ggggtctcgc cccgggcctg aataaactgc ctttgacaaa
    27181 gaataaggaa gacctgcctt tccgctattt tgaaaaattg ccggaccagg tgctccggaa
    27241 attgcaggag gaaaccctga ccgcccggtc caaacccggc tggctggacc gtatcctgca
    27301 ggaatggctc cctaaaaaat atatggtact ggctatggct tccctcctga ccgccatcct
    27361 ggtcattggc ctttggccgg ggtcgacccc aaacctgccc gaagccgatt ttgcaagcgt
    27421 cgatcgcgga gaagccaggt cttacctcct gacctgcgcc gaggacctgg atgaaagtca
    27481 tttagctctc ctggtcagcc aggttgaagg agatgatttt atgcccctct ccgacgaaga
    27541 actggatgcc gtgatcgaag aatatctcta ccagcaagcc tcagatatgg aaatgaacta
    27601 aaccacttat actgtacaca ataaaatgat ccgaacatga aaaccattcg caacttcctg
    27661 ctctactcca ccctgctgat ttattccatt cacggataca gccagcgcgg tgaaaagata
    27721 gaagccctcc gcatagcctt catcaccaat aagctttcgc tcaccgccgc ggaatccgaa
    27781 aaattctggc ccgtctataa tatttatcgc cgggaggtgg ccgagctcag gcgacaatcc
    27841 ttcatcgaca ccaacctcga agccatgagc gacgcggacg ccgaaaaagc catccaggtg
    27901 accatcgacc ggatggagaa ggaactcctg ctctttaaaa acctggcccg ggacctcaaa
    27961 cccatcctcc ccgcacgaaa aatcgccctc ctttcaaagg tcgaacgcgc ctttaatgaa
    28021 gaaatcatcc ggcgcagcca gatgggaagg aattaaagga gaaaggtcga gggggaacgg
    28081 ttaagggtta aggggcaacg gagcataagt gaccgacaac gctgaaagct gatagctgaa
    28141 cgctgatggc tgatagctcg ccgctcccgc ctatctttgc gatatgtcaa aatccgatca
    28201 actggaccaa aacatcaggt ggaccacaga actcatggaa atgtcccctg atcaattgct
    28261 gaaacaggga cctgagcatt acgaccagct taagtccgtg atccggtttc acgagcacag
    28321 gtattatatc ctgaacgatc cgttgatcag cgactatgaa tacgaccagc tctataagaa
    28381 actggaattt ctggaggaaa aacatccgga atggattagc agggactccc ctacccaaag
    28441 ggtgtccacc gacctcaccc aggcattcga gacggtagat caccttaaac ccatgttgtc
    28501 cctggccaat tcttatgatg aaaaagacct gttggatttt gacaagcagg ttaaaaagct
    28561 ggcccaatta ccggcgaccg acaaggtgac ttactgtgtc gagcctaaat acgacggagg
    28621 cagtatttcc ctggtctatg aaaatgacca gctggtgcgc gcggccaccc ggggtaatgg
    28681 tttccagggt gaactgatca ccgctaatgc gcgcaccatc aaaaccatcc ccctgaaagt
    28741 cccattcaaa aaagatggca ttgccagggc ggaactccgg ggggaagcgc tgatccgcaa
    28801 ggacaccttc cggaaaatca ataagcagcg cgaagaggaa ggactggtgc tctttgccaa
    28861 tcccaggaac gccgccaccg ggggccttcg gaccaaggat gccagtgaaa cggcaaaacg
    28921 cggcattgaa gccgtgattt accaggtggg ttacctggaa gatgaaaaag gaagggaaat
    28981 caacccctac aaggaccacg ccggcggcat tcacgccctg gcagccatgg gtttcaaggt
    29041 gcccaaggtg gaatttaaag tctgccatgg gatccatgaa gtgatcgaat tttgccacgc
    29101 ctggcaggaa agacgggatg atttttattt tgaaatcgat ggcatggtcg tgaaggtgaa
    29161 tgacctcggc ctgcaagcca gcctgggcta cacttctcat catccccggt gggccatagc
    29221 ttttaaattc aaagccaaac aggctacgag ccgcctcatc ggtgtggagt accaggtggg
    29281 aaaagtaggt acgatcactc ccgtggccaa actggaaccg gtacaggtgg gcggggtcac
    29341 ggtctcctcc gtttccctcc acaacgagga ttttatcaat gccaaagacc ttcacatcgg
    29401 ggatatggtc atcgtagagc ggtccgggga tgtgatccct tatatcgtaa aagccttgcc
    29461 ggaataccgc aaggggtatg agaaaaaaat aaaatttccc gtctattgcc cgatcggggc
    29521 tgaattccat gtcaagctgg tacgcgaacc gggccaggct ttctggatct gtccggattg
    29581 taaatgcggg gcccaaaacc tggaaaaata tattttccat gtgtccaagg cggccatgga
    29641 tatagaagga ttcggcccat ccatcatgga gaaatttgta caattgggat ggattaaaag
    29701 tttggcggac atctaccggc ttgactttga gcagattgaa aaactggaag gattcggtga
    29761 aaaaagcgca ggcaaactga aaagcgcgat tgaaaaagcc aagtctaatc ctattcagcg
    29821 tctgctccac agtctgagta ttcaccatct gggcaaaaaa gcctcctccc tgatcgccga
    29881 acgggtcggt tatgtgcccg acctggccaa ctggacagaa aatgatttta ccaacatccc
    29941 cgaaatcggg ccggtagtca ccaaaaatgt catggattgg ttcggtaacc cgaaaaacct
    30001 ggaagtattg aaagagatgg aagccttggg ggtgaacatg taccagacca gggaagacaa
    30061 acccaaaaaa gttgatgccc acgcccccct ggccggaaaa accattttat tcacgggttc
    30121 tctttcgcaa ctcagccgtg aggaagcgga aaaaaaggcg gaggacgctg gtgccaggaa
    30181 cctgagcgga gtgagctcca aactcaacat cctcgtcgta ggcgaaaatg caggatccaa
    30241 acttgaaaag gccaggtcat taggtaccgt cgaaatctgg actgaagatg aatttattca
    30301 aaaggtcgcc gaaacctgac cggatccgcc accatgaaaa acctgtcttt gttattcctg
    30361 tttcttgaag ccatcctgat tttcccaaac ctgaactgtc gcagatacaa accattaaaa
    30421 atggaaacgg actataccaa ggaggggatg atcccggttc agggcacctc ccttcattat
    30481 ttgcaatggg gtgagggcgg caaagccctt gtgatcttgc atggactggg agattcgcca
    30541 tatatttttt cacacctggc ccgggagtgg tcatcccgtt tcaaagtcaa tgcctttgcg
    30601 cgccgcggtc atggaaggtc cggcagcccg gatagcggat acgataatca aatgctgacc
    30661 aacgacctga aatccctggt ggaccacctg caactggacc gtgttttctt attaggctgg
    30721 tccatggggg gcaatgagat cacggaattc gctatccgtt acccggaacg gactgcgggt
    30781 ttgatttatc tggaagccgg ttacgattac agcgagcctg cttttgcctc catccttgat
    30841 cagtttccgg aagccctctc ccccggaccc tcagacctgg tatccctgga tgcataccgg
    30901 caatggtatc accggttctg gtatccggac ctggattgga acgaagtcct ggaatccaat
    30961 ttaaaagcct gcacacgggt actggaaaac ggcgcggttg aaattatccc aggagaaaag
    31021 ataacaaacc tattggtccg gtccctcacc gcttatccca gggattattc caaaatcacc
    31081 gctccggtac tagccctata cacccctgtt ttttttaaac accattccgc ggacccaaat
    31141 ctccaaaagg cctatgaaga cctggaaaac aagatcatca gtccctggag ggaatccaat
    31201 aaagaaaagc tcaaaaaggc tttaaaagag gtacacatcc atgaactgag tatgggaggg
    31261 catgcctccg tattattcgc ctcgcaggac agcgtgttaa aactggtcaa tgattttgta
    31321 gccgcccact gatcccgaaa attcttcaat gtccgttatt acaatgccat atggaaaaaa
    31381 taaaaatcgc caccgcccag tttgagcacc ggagcgggga caaagcgtat aacctgggtg
    31441 tcattgaaaa actggcatac caggccaggg accagggggc ctcggcgatt gcttttcatg
    31501 aatgttcggt taccggctac acctttgccc gccgccttga ccgggaacag atgctggacc
    31561 tggctgaatt tattcccgac ggccccagta tccggcaact gacggccatt gccagggata
    31621 ccggagtcac cctcctcgcc gggctttttg agaaagacag ggataaaaat ttatacaagg
    31681 cttatgtatg cgtcaatgaa caaggcctgg tggccaaaca ccgcaaactc catcccttta
    31741 tcaatccctg gctgaaaccg ggggattcgt attgtgtgtt caaactgcat ggctggacct
    31801 gcggcgtact gatttgttac gacaacaata ttgtagaaaa cgtaagggcc actgccttgc
    31861 ttggcgcaca ggttcttttt atgccccatg tgaccatgtg tactccctct ccccggcccg
    31921 gggcgggttt tgtggacccg gcgctttggc acaaccggga agcggatcca accagcctgc
    31981 gtctcgaatt cgacggcatg aaaggcagag cctggctcat gaagtggttg ccggcgaggg
    32041 cctatgacaa cgggatctat gtcgtctttt ccaaccccat cggcatggac gacgaccagt
    32101 tgaaaaacgg gtgttccatg atcctggatc ctttcgccga tatcctggcg gaatgccgca
    32161 gttttgagga cagcctggtc acagccaccc tggtgtccga aaaactgggg cagtccggcg
    32221 gcagccgtta tatcaaggcc agaagacccg aactttaccg ggatatcctg gggcaggaac
    32281 accagggtga acaaaaggta gcctggatga gcggggatta aggtggagcg cacggcttac
    32341 ctgatctccc gatatatcat tgagaacgga ctgctgagtg ctcatagcgt acagatcagg
    32401 tattactgcc aggatcctta tttcccccgt gggcctgccc caatagtgat atttgaaaaa
    32461 tagtactcga gggcctgccc caactgtgat atttgaaaat tagtactcgg gggccagccc
    32521 caactgtgat atttgaaaat tagtacttgg gggctggttc agccccctat tattcagaca
    32581 ataattatct aagttaagaa atcaattg
//
LOCUS       DAOQXT010000047        32449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1610_length_32449_cov_6.189973, whole genome shotgun sequence.
ACCESSION   DAOQXT010000047 DAOQXT010000000
VERSION     DAOQXT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 32449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32449
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1610_length_32449_cov_6.189973"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            858..1577
                     /locus_tag="PKM27_09820"
     CDS             858..1577
                     /locus_tag="PKM27_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNR07601.1"
                     /translation="MYKNPSIPVLLILLGLGLVISACSVQSARNPIKDTKIVDKPITW
                     DTTRKRLSLEYMQIRHGMTNVRAPLITPSMVVVHWTAIPTLAASYNTFRPSVLEGREE
                     LQGASKLNVGVQFLVDRDGTIYRLLPETAFARHTIGLNHCAIGIENVGDGDQYKLTDA
                     QLRANVTLIRYLHYKYPIRYVIGHMDYGRFQTHPLWKETDPNYLTMKTDPGEDFMKKL
                     YNGLSQAGVFGISRFPDAKIN"
     gene            complement(2235..3197)
                     /locus_tag="PKM27_09825"
     CDS             complement(2235..3197)
                     /locus_tag="PKM27_09825"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF009251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNR07602.1"
                     /translation="MSEFYSPIGHTDFHFSPDQGVYKGKVREIYTVGDRLISIASDRI
                     SAFDHILPRLIPYKGQVLNQVAAHFLEATRDLVPNWLESVPHPNVSIGKKCKPVKLEF
                     VVRGYLAGHAWREYQSGKRMICGVHMPEGLKESDRFPQPLITPSTKAEQGHDEDISKE
                     QAIAQGLCSLEEWITMERYALALFNRGTEMAADQGLILVDTKYEFGRYNDQILLIDEV
                     HTPDSSRYYYLEGYAQRQESGERQKQLSKEFVREWLMAHGFAGKAGQVMPDMPDAFVW
                     EISDRYIELYEKVTGKAFQKDPDNQNFHAIEQAVRQALKSRPSL"
     gene            complement(3252..3659)
                     /locus_tag="PKM27_09830"
     CDS             complement(3252..3659)
                     /locus_tag="PKM27_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07603.1"
                     /translation="MHDVLVKIRFIFYRFHDKGLEFFLVKENDQWTLPSGHAKDQTQR
                     FFNREGEMIELEADEIDRMKNLAIEADWHDMPSIRGLIKHDLKMVEEKFLQLEEGSYL
                     AAKEAFKKVLPQEYALLKELKDILFDRNLTQNI"
     gene            complement(3739..4812)
                     /locus_tag="PKM27_09835"
     CDS             complement(3739..4812)
                     /locus_tag="PKM27_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNR07604.1"
                     /translation="MTPLSSEQFLENYINNPSPTGFEVKGQQLWLEYVKPFTDSWELD
                     DYGTAFAVINPGQDYRVVIEAHADEISWFVNYITDNGLIHVIRNGGSDHQIAPSKRVN
                     IFTDKGIVKGVFGWPAIHTRSSTAEDPKLSIENLFVDVGAKDKEEVGKLGIHVGCVMT
                     YEDDFFVLNENYYVGRALDNRLGGYCIAEVARRIKENKIELPYSLYIVNAVQEEVGLH
                     GAEMIARRIKPNAAIVIDVTHDTSTPHMNKKKDGDVKCGDGPGITHAPSVHQKLLRLV
                     TDAAMVNGIPFQRQASSRATGTDTDAFAYSNQGVPTVLISIPLRYMHTTVEMAHKQDV
                     ENVIQLMIHSLGKIEKNHNFKYF"
     gene            complement(4831..5469)
                     /gene="pyrE"
                     /locus_tag="PKM27_09840"
     CDS             complement(4831..5469)
                     /gene="pyrE"
                     /locus_tag="PKM27_09840"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNR07605.1"
                     /translation="MLTPEHIAYRLLQIKAIKLSPQKPFTWASGLKSPIYCDNRLILS
                     YPEFRSQVVDAFIRESRPFVPFDVIAGVATGGIAYGALIADRMELPMIYVRSEAKGHG
                     LGNQIEGRLEAGQRVLVIEDLLSTGGSSLKAVQALRDAGAVVTGLLAIFSYGFEACTR
                     AFKEAEVKAQTLTDYPTLLRVASSNEVISRDDFDLLQSWNKDPKAWSEKHSV"
     gene            5468..6103
                     /locus_tag="PKM27_09845"
     CDS             5468..6103
                     /locus_tag="PKM27_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNR07606.1"
                     /translation="MNLRAQKYKFCINTTWILILDKKRLHKLSMKDFPMALVLQEPGM
                     ALIRETMHQLLDDIIRMPVIISTPDPKAVWEKILVKNTLIKAAGGVVKNPQGKFLLIH
                     RRGWWDLPKGKIDAGETPRKAALREVKEEVGLDCRILSALPDTWHIYRLNGELILKKT
                     YWYLMASSNSRTRLQEVEDITGHKWFTNNKLIMFKNKVYPNLADLLQFVAQ"
     assembly_gap    6448..6547
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7437..8585)
                     /locus_tag="PKM27_09850"
     CDS             complement(7437..8585)
                     /locus_tag="PKM27_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNR07607.1"
                     /translation="MPSVHSIPDLQLIQQTCRDFALRNIKPKSMEWDESQHFPAELFK
                     ELGTLGFMGVLVPEQYQGAGLGYQEYITILEEISKVDPSIGLSVAAHNSLCTGHILQF
                     GNEEQKEKYLPLLATGEWIGAWGLTEPNTGSDAGRMQCIAREDGDHYIIQGTKSWITH
                     GKSGDVAVVIARTGELLDNNGMTTFIVERGTPGFSGGKKENKLGMRASETAEMIFDQC
                     RVHKSQVLGKPGDGFRQALGVLDGGRISIAALSLGIAKGAYEASVQYAKERHQFGQAI
                     AHFQAIGFKLADMQTKIQMAELLTRKAGSLKDSGEKMTREAAMAKYYASEICVQVATD
                     AVQIFGGYGYTKDFPVEKFFRDSKLCTIGEGTSEIQKLVIAREIGKNR"
     gene            8732..10531
                     /locus_tag="PKM27_09855"
     CDS             8732..10531
                     /locus_tag="PKM27_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07608.1"
                     /translation="MMNFRTLFFLLFAGLPVLAAADNYLVTVYGRLVDGGGNGIGNAV
                     IYISQAPGSPFTVNEKVSTLPSGEFKWPVYIPDSIPGGVLLLGFQNCEGKEIFARFEF
                     SKNKPVIEAKLIFCERPVGKPCASEVIAKKINDTLAIAWVRAKGIPPFMHQWTNGLTG
                     DTIRFNPQLKIRICVVTKDSTGCKSEACVPGFNNSCGIYIEQDGRFLIAKSKEERIVS
                     LLWSTGADGEKIEIKEPGEYCVKAVMASGCEAKACILVKPVNLDPCAAEIVAERLGSD
                     NQDSTPGIRLQVKSGFDLKYILWSTGETTRTIVVRKSGEYCVSVSDNVRCKMYICKKV
                     ELGDDSCRVFIHMEKIPVTSNTDAGHKVRLTAKTTFKPNLYYWNTGDTTPSILVEKSG
                     EYCVTVSNGVNCKTASCVKVDLPGPNISRCEASIVVYQKTTGELDLITKAAGKAPFTY
                     QWITGSTDANITVKNSGKYCVTIKDAAGCTMQACVEVLFEKPSGGNLFPDADKIKLGV
                     QPLAEGAQIQLNIHPNPTADRLVYQVNSKLSEAGQLSVMNLYGKTIYQEKIDVQDGLS
                     YGQLDLSFLPPGVYMVNIHQQGFISSKKLIIER"
     gene            10594..11190
                     /locus_tag="PKM27_09860"
     CDS             10594..11190
                     /locus_tag="PKM27_09860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNR07609.1"
                     /translation="MQIDEDILKGCRKGDRRSQFRLYELCYPYLMGVALRYRKQREEA
                     ASAVNMAFLKILNHLDRFRENHSFKSWMRQIMVNTLIDEFRSSARFSSESGAVDYEKE
                     VAVNGVHVDYNNAEINLNVEQLVSYIHELNPLTASVFNMYVLDGFPHKDIASMLSISE
                     AASKWHLFTARKQLQERVETASERNTNPLIPKIKVNPV"
     gene            11187..12485
                     /locus_tag="PKM27_09865"
     CDS             11187..12485
                     /locus_tag="PKM27_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07610.1"
                     /translation="MKNEPWIDRVYKESTETWDVPVNLDHWQQAASLIDRRDKKKKRA
                     VWIRMAGLSGAALILLLILILWRNGPSESSPAAEPQPAGSPAAEPMAQASGTTDSPAP
                     GFPVQAIGPIAENTSGQGSSSNPSSQAYFSNNQAPRTIGLKNKEKIRQAKPYPADPSL
                     SSSIRINAPGLSTEAATPATPVPQRESLPEEGLDPLDFMAMTSSHKTLPVTVLEIPVE
                     VDYKPFRRKEFGLRLDLTRQAENGRSASMSMAGLEVFARKTLGTKTYWDLSLGYNSFF
                     NSSLYSEVLTSYQFQGFGSTVKNYGVKPEWIHYLYLQMAGGMQLNKHRAFAGIRPQLL
                     LGAIGEVDQIRFKDGAAVRDLSSAEISNLANGWLDKGSLHQLVFNVHLGYEYQFHPKF
                     GIGLQASRLLNDLYRPLPGDIAQHAGAKWNLGMGISYKLK"
     gene            12488..13597
                     /locus_tag="PKM27_09870"
     CDS             12488..13597
                     /locus_tag="PKM27_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07611.1"
                     /translation="MRPLKYIGLILTGFLSVTCNKIELPLNPAETPIFEMKANFGNQA
                     VNLAAGVDGLELDTRFISENLDVLILQGELKKKNCNPPCPNSLRLSLRQNRQDFLGNS
                     GIGLNPGRRPFYREARDSFIVHGKSLSQLPNSGSGRYDFGWTLNDKKVADKEIVSFPF
                     NPQIKNTVCLYVSHPDGSKASQCQVIDYSVKDSFPGLRVSIETQYNQSRGWTLVPKVR
                     GLGPFKYKWDNKSADAVQELDPKQASSHCVTVQDARGNTASACVDFNPEGKVKCRADF
                     ELTADISRLNQWTQYNTTEIVFVNEGGQKYLSSLMPQPASSVFEILESAPFEENAQQQ
                     PTRKLKIHFDLLLFGPDRTAIPFKGTGTIAVAHPK"
     gene            complement(13598..14371)
                     /locus_tag="PKM27_09875"
     CDS             complement(13598..14371)
                     /locus_tag="PKM27_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNR07612.1"
                     /translation="MLLIFLLFVSQMVQQPLIRFHENPVVAHRGAWKKNNLPENSIAS
                     LREAIRLRCTGSEFDIRMTNDEVLIVNHDPEFHGMPIEKTNYSQLAAIRLSNGETLPT
                     LENYLLEGMKENPGTRLVLEIKPSPAGVEKGRKIAKRVVEMIQNLHAEPWVTYISFDL
                     EILRAVLALDPQAATQYLNGERSPGELKQDGISGLDYHFSVFNKNPGWVVEAKKSQLA
                     LNVWTVNSAEDLERFVSQSFDFITTNEPELLFDILKAKP"
     gene            complement(14408..15658)
                     /locus_tag="PKM27_09880"
     CDS             complement(14408..15658)
                     /locus_tag="PKM27_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNR07613.1"
                     /translation="MRPRTLDEFAGQSHLMGPEAVLRKLIESGNTPSMILWGPPGSGK
                     TTLARLIAATQQRPYFPMSAIESGVKDVRETIEKAQRHRFFDKPNPILFIDEIHRFSK
                     AQQDSLLHAVEKGIINLIGATTENPSFEVIPALLSRCQVYVLNPLSETELLQMARHAC
                     VQDEYLKTRHWIFTETDALIHLAGGDGRKLYNILDILYSAHTGAGDIQVTNLAVSQAV
                     QQNLARYDKGGDQHYDMASALIKSVRGSDPNGAVYWLARMLEGGEDIEFIARRLIVSA
                     SEDIGLANPNALLIAQACFQAIHNIGMPEARIILSETVIYLATSPKSNSAYMAIEDAT
                     RLVRQTGDLPVPLHLRNAPTRLMKSLDYGKDYLYSHSFEQNFAAQEYLPDPVSGTRLY
                     SPQDNPTENKIQERILSWWKDKYQ"
     gene            complement(15749..17140)
                     /locus_tag="PKM27_09885"
     CDS             complement(15749..17140)
                     /locus_tag="PKM27_09885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR07614.1"
                     /translation="MSKSIFNRSIPAPRFLLILAVLLSGFGLEAQKQVIDKVICEVGN
                     EVILLSDLAEQIKMTESRQGPMRPEDKCFLLENMMISKLLVHQAFVDSVIVSEEELES
                     QLDARIERILESMGNDVTQFEAYYGKTTSEVRDDFREDLRNQLTAERMRTQLMEEVLI
                     TPSEVRQFYESIPKDSMPYLSSEVEVSEIVLYPVPNEESKKRAKDKMDKIIARLKAGE
                     KFEELAATFSDDPGSARNGGDLGWAKRGSFVPEYEAAAYNLEPGQVSEIVESEFGYHL
                     IQLLELRGNTIHTRHILIKPEIEVQDLDLARNTLAKVRMMILNDSITFSKAVKQYGHK
                     KEQSYSNDGRITNPRSGDTFFEIRDLEPEVYFTLDTMKVKGISSPVELIAPTGEKYYK
                     ILLLNSRTDPHQANLKQDYAKFQALAKESKKSELLVKWLETHIESTYIRVDPLFLNCP
                     NIQQWIVKQKNEN"
     gene            complement(17118..17978)
                     /locus_tag="PKM27_09890"
     CDS             complement(17118..17978)
                     /locus_tag="PKM27_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07615.1"
                     /translation="MRPLHLHFFLVLTVWTAACQFIAPDPGAGDPVLASVYSKKLYLS
                     DLEGIFPPECTREDSTRIISNFTSRWTQEQVFLNEAEKHVPADLKIEQLLKKYKESLI
                     GLNFQQKLIEQNLDSMVSEQELRDFYEKNKDQYQLETSIIRCYFVKIPANIDEMRRAR
                     EWWDDLEKNNNKKRLAGLADKYNGIYHLEDSVWHNFSDIEALFPKDRISARNWSRNER
                     LSISDAEFQYFFKVLELVSTRENAPFSYVAEKAKLIILNKRKNKLVEEFKTKLYETEL
                     RKNNVKIHIQ"
     gene            complement(18006..19982)
                     /locus_tag="PKM27_09895"
     CDS             complement(18006..19982)
                     /locus_tag="PKM27_09895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR07616.1"
                     /translation="MKFSKRTSYTLIFILWCTVFASGQQADEILFSVNGQGIGVSEFL
                     YIYGKNNAKDADFSRKSLQEYLDLYKKFKLKVAKARAMRLDTLPELMEELNTYKQQVS
                     NSYIMDKEVTEQLIREVYERQRNDIAVKHIFFQLSPNAPPQDTLAAWKRAMKAYGELT
                     GGVPFGKVVQENSDDRSNINHSGDLGYVTSMLPDGFYEVENTIYSLQRGAFSKPVRSS
                     AGYHILRVDDIRPARREMEIAQILIKKPAEREHDQGGKPTAEKIYKMLKNGADFAELV
                     RTFSHDESTKAKDGYIGFVGINQFEKSFEDAIFQLEQDGDISMPIESRVGWHIIKRIS
                     RKAELPYEESREKIKAQVEADGRFAYARNRVIDKIKQANGFKVNQEHLNTFIQSLPPD
                     FTSYSWEVSPSLPDLDLFTLSSKSYGTREFAHYLKQNTRERLQYAAGTPASQVVPGIF
                     NTYVTEKTIEFEQDHLAKTYPEFRNLMREYEEGILLFEITRQSVWDKASSDTLGLNQF
                     FNKNRNKYKWGERALVTDYTVASKDEATIKAIYEMSRKKEVDKLAKNLEKSYPGLVRF
                     EKTFAEKPEAGTAEVPWAAKQVTPYSIDKENGIATWRKVEKIVPPQLKSLDESRGYVI
                     ADYQDFLEKAWITDLEKEFPITLNQGVFESLIKK"
     gene            complement(20222..21901)
                     /locus_tag="PKM27_09900"
     CDS             complement(20222..21901)
                     /locus_tag="PKM27_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNR07617.1"
                     /translation="MARIFLLVSLFFVLNPRSFTQCLQGNCNNGSGTYVYPSGARYSG
                     EFVNTKIHGKGTLWMSNGNIYTGEWVQNYREGRGKMVFKNGDTYTGEFLRSKLHGTGT
                     MQYSLGGQYSGEWKSDLPDGKGKYTYANGETYEGLLTEGRRNGYGRYHYSDGSVYDGQ
                     WRDNLREGQGSLLLANGKKTSGNWSGDRLLAEGESLAASQENLPENVNANAALPTLST
                     PNLQNCNEIRCMYGLGFYIYKDGSKYVGPFRDGNPYGRGIVYYANGDRYEGQWDDTAP
                     QGEGIMYFRSGKVFAAVWDRGRPVRQLENRPAIRPRTDIRPETDEEVRVWAVIVGVAR
                     YAHMPVLKYADDDAYQIYAFLKSPEGGAISDHQIKVLIDEDASRQKIIDALQEQFGKA
                     DANDVVMLYYSGHGVNGAFLPIDYDGYNNKLFHEEINQIMEESEAKHKLCMIDACYAG
                     SMADAKADFSASLNKFYSILEAEKGGAAFILSCKNREVSLEDSGLRQGIFSHYLIRGL
                     KGEADGDRNKIVSVQELFNYISQEVKTYTGNIQNPVMEGNYNPNMPVAILR"
     gene            21989..22486
                     /locus_tag="PKM27_09905"
     CDS             21989..22486
                     /locus_tag="PKM27_09905"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNR07618.1"
                     /translation="MRIFSLLTAMVLMAGACKNLPTNETLSSQAGGIKSFGAEITEDG
                     FVTLDQATAMLGGVDSLPLKLSGTVKEVCQNKGCWMNVVAESGESQTVFVKFKDYGFF
                     VPKDCAGKKVVMEGIAYREVTPVDELRHYAEDKGASAEEIAAITEPKEELKFMASGVL
                     LYEKE"
     gene            22587..22662
                     /locus_tag="PKM27_09910"
     tRNA            22587..22662
                     /locus_tag="PKM27_09910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22620..22622,aa:Gly,seq:gcc)
     gene            23253..23338
                     /locus_tag="PKM27_09915"
     tRNA            23253..23338
                     /locus_tag="PKM27_09915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23287..23289,aa:Leu,seq:taa)
     gene            complement(23715..25970)
                     /locus_tag="PKM27_09920"
     CDS             complement(23715..25970)
                     /locus_tag="PKM27_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNR07619.1"
                     /translation="MTTVKTTINRRSFIKVTSLAGGGMMLSFNWLAGCKPTSQEILAL
                     PKEWFELNSYIKIGENGAVTLMSPNPEFGSNVKTSMPMILAEELDIDWKNVRVEQADF
                     FPERFERQFTGGSQAIRQGWKILRTAGATARQMMIRAAAQTWNVPVEEITTEAGTLQH
                     LPSGKKAGYGELASLAATLPVPEEVPLKKVKDFKIIGTSRKNVDGLKIVTGKPMFSLD
                     HTQDGMLVAMIAHPPAFGMKVKSVDDTAARAMPGIKDVFTIKTLADEYERNGFDTTSF
                     TELVVVAGNTTWEVMNAKKALVIEWEKISDSHIVVNGWGGKQTVRIPAGLESTAGHKA
                     QMAEMAKKPGKVLRKDGDPEAAFKRAAKVIERTYSAPYLAHNTMEPVNCFARVTDEHA
                     EIYGPIQAPESIMQTLVARLGMPKEKIHIKLARMGGGFGQRAYGHHMVEAAVISQKLK
                     APVKLVYTREDDMTYGIYRPTYTATYRAALDEKNQLLAFHVKAGGIPETPIHPNRFPA
                     GAIDHYLAESWSIESNITIGAFRAPRSNFMAGAEQSFLDELAEELGKDPIEFRLELLD
                     RAKTNPVGENNDYDPDRYAGVLKLVREKSKWQEPQPNIHRGVSAYFCHNSYVAEVLDL
                     VIQNKNPVIQKVYAAVDCGIVVNPDAAANMGEGGIVDGIGNALYGEMTFQDGVPQKTN
                     FDTYRMIRQVEAPKSIEIHFVENETDPTGLGEPLFPPVFAALSNALYKATQKRFYEQP
                     FISGLDQGFAD"
     gene            complement(25992..26447)
                     /locus_tag="PKM27_09925"
     CDS             complement(25992..26447)
                     /locus_tag="PKM27_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR07620.1"
                     /translation="MAIFNLNVNGKKTQVDLDPATPVLWVLRDHLNLVGTKYGCGIAQ
                     CGACTIHLDGVATRSCLLPVAAVGNKAITTIEGLSENGDHPVQKAWLEHDVPQCGYCQ
                     AGQIMTAVAFLKNNPHPSDEEIETAMNGNLCRCGTYLRIRAAVKTASKS"
     gene            complement(26634..28880)
                     /locus_tag="PKM27_09930"
     CDS             complement(26634..28880)
                     /locus_tag="PKM27_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNR07621.1"
                     /translation="MTLVKTFNGRRSFLKATALAGGGMMIGFSWLPSCQSDSNADLTL
                     PKEWFDLNGFLKIGDNGLITIMSPNPEGGQNVKTSMPMIVAEELDVDWEKVVVEQAPL
                     DTKLYTRQFIGGSQAIRQGWHGLRMAGATARHMLKQAAAQAWQVPETEITTAEGVLLH
                     EKSGRSAGYGEMAAAAARIPVPKEVPLKDTKEFKLVGTSRKNVDGKKIVTGQALFGMD
                     YHRPGMLIAMIVHPPAFGMKLKSFDDQSARKMPGIRDIFTIKALQDEYERQYFDTCTF
                     TDLVAVVGETTWEVLNAKKALVVEWELFTAYTESKLTPGGKQTVRFPAGLENSSEHIL
                     QMEKEAAKPGKVRRKDGEPEAVFKLPARVIERTYTAPFLAHNCMEPMNFFAHVTADKA
                     ELAGPLQKPEYTEKTLSARLGLPLEKIDIQMTRLGGGYGRRSYAHWLVEAALISQKMN
                     APVKLIYTREDDMTSGIYRPAYHAHYRAALDAENNLIAFQVKAGGIPESPLYADRFPA
                     GAVDHYLAEDWAIESNITVGSFRAPRSNFMAVAEQSFLDEVAEAAGKDPIEFRLELLK
                     KAKNHPVGKNNDYDPDRYAGVLELLKEKSNWEEEIPGIYRGVSAYFCHNSYVAQVLDL
                     VMEQGKPKVAKVTCVVDCGIVVNPDAARNLTEGAIVDGIGNAMYGSMTFRQGEPEMNN
                     FDKYRLIRMEEAPGAIDVHFVKSDIDPTGLGEPAFPPVFGALANALYKATGKRHYHQP
                     FIGNGLAG"
     gene            complement(29041..30036)
                     /locus_tag="PKM27_09935"
     CDS             complement(29041..30036)
                     /locus_tag="PKM27_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNR07622.1"
                     /translation="METRRLGKNGLEVSALGLGCMGMSFGYGPPQNKREMIALLRAAV
                     EKGVTFFDTAEVYGPFTNEELVGEALAPFRGTVKIATKFGFKPSASDPAKWNELDSRP
                     EHIKQVAEASLKRLKTDAIDLFYQHRVDTGVPIEEVAGAVADLIREGKVRHFGLSEAG
                     LQTIRRAHAVFPVTALQSEYSLWWRKPEEEVIPTLEELGIGFVTFSPLGKGFLTGAID
                     EHTLFDKTDFRNVVPRFAPEARKANQGMVDLLGNIAKEKNATPAQIALAWLLAQKPWI
                     VPIPGTTKLNRLEENLGATSIELTQDDLREIEHAASQITITGARYPESSERMVDR"
     gene            complement(30117..31016)
                     /locus_tag="PKM27_09940"
     CDS             complement(30117..31016)
                     /locus_tag="PKM27_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR07623.1"
                     /translation="MDKVESLEEFYKRKIGWMPDPIREEIGHFNVFKLDPFVGKNAQP
                     VPYKRRDFYKIMLVVGKSKVHYADQVVEVAKQALTFSNPQIPYKWEHIDQIRRGVFCI
                     FNQHFFHRYGNLNQYSLFQPGGNHVFELSDRQVNQVSGIFQRMMEEINSDYIYKYDAL
                     RTMVFELIHFAMKMQPSAKIDHQALNASQRISTLFLELLERQFPIDDNHKAVNLRTAS
                     DFARQLNVHVNHLNRSVKETTGKTTTQILAGRLLQEAKILLKHSAWPVSDIAHALAFR
                     EVTHFNNFFKKHVKLSPIKYRSI"
     gene            complement(31130..31807)
                     /locus_tag="PKM27_09945"
     CDS             complement(31130..31807)
                     /locus_tag="PKM27_09945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR07624.1"
                     /translation="MTRSRRWVIGDIHGCNRTLNGLLFNKLKVSSKDELFFLRDYIDR
                     GPDSKGVIDTLLNLKERQIRVHALLGNHEQMMMESEHDELLLEFWIRNGGDATLESFG
                     LESYSSFPDPYKAFFTDLPYYAETGKYILVHAGLNFEEPDLFSDEYLMLYQRIQDTNQ
                     AALGDRVMLHGHTPLPLGQILAQTANCRNLDGGCVFSRRRKDLGYLVAYNLDDGDYVY
                     EKCMDWD"
     gene            31944..>32449
                     /locus_tag="PKM27_09950"
     CDS             31944..>32449
                     /locus_tag="PKM27_09950"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNR07625.1"
                     /translation="MSKHGRVLVAMSGGIDSTVAAMLLHEQGYEVVGITMKTWDYANS
                     GGAKKETGCCSLDSINDARTVAVKMGFHHFILDIRNEFGDSVIDNFVEEYLAGRTPNP
                     CVLCNTHIKWEALLKRADALDCPFIATGHYARVGELEGRKYISRAKDTSKDQSYVLAV
                     LTAEQLRHA"
BASE COUNT         7568 a         8208 c         8292 g         8281 t
ORIGIN      
        1 tcattcttgc ttttaccggg ataagcgcgg agatgggtca attaaaattt gtttcggatg
       61 tcaagggttg ggtacccctt cagggcctgc cccaacttta ggatttgata attagtacgt
      121 gggggacagg ggtcggagaa caaaccatta ataaccaaaa tttttaagat ttattcaacg
      181 cccctgaccc ccctgattac cattttcaaa aactacagtt ggggcaggcc taaaggggaa
      241 ccaaagatcc tggcagtata ctcaaataaa actttcttcc ctgcccttac cggaatgagc
      301 gaagagatag gtcaattaaa tttatatcgg aatgccatgg gtcgggttcc ccttcaggtc
      361 ctgccccaaa tgtaggattt gagaattagt acatgggggg ggacaggggt cggaatacta
      421 tgtaaaaaag aaaattcata taaaattatt tcaacgcccc tgacccccac aggggggacc
      481 aaggagcctg gcggtatccc tgaattataa caatcatcct tgcccttacc gggataagcg
      541 cagagaagga tcaattaaaa tttgtttcgg atgtcaaggg ttgggttccc cttgagggga
      601 caggggccgg agaacccatt aataaccaaa attttgaaaa gagaattttc cattgtccct
      661 gtcccccacg ggggaaccaa ggattcgggc agtatgaggt cagaggtcag aggttagagg
      721 ttagaggtca gaggtcagag ttagcccacc atccaccacc caccatccac cacccacccc
      781 tcaccaccca ccacccacca ttcatacttt cccccgtccc tttttcgtta acatttcata
      841 tcttaacccg gcaacttatg tacaaaaatc caagcatacc ggttctgttg attttattag
      901 gacttggcct ggtgatctcg gcctgctctg tccagtccgc aaggaacccg attaaagaca
      961 caaagattgt tgataagccg attacctggg atactacgcg caagcggctc tctctcgaat
     1021 acatgcagat ccgccacggt atgaccaatg taagagctcc cctgatcacc cctagcatgg
     1081 tggtggttca ttggacggcg atcccaacct tggcagcctc gtacaataca ttccggccca
     1141 gtgtgctgga aggcagggag gagctgcagg gcgccagtaa gctgaatgtg ggggttcagt
     1201 ttttagtgga ccgggatggt accatatacc gcttgttgcc ggagacggcc tttgccaggc
     1261 ataccatagg cctgaatcat tgcgccatcg gtattgaaaa tgtgggggac ggggaccaat
     1321 ataaactgac ggacgcccag ctccgggcca acgtgaccct gatccgctac ctgcattata
     1381 aatatccgat ccgttatgtg atcgggcata tggattacgg ccgctttcaa acccacccgc
     1441 tctggaagga gaccgacccc aattacctga ccatgaagac cgatcccggc gaggatttta
     1501 tgaagaaatt gtataatggg ctgagccagg ccggggtttt cgggatttcc aggtttccgg
     1561 atgcgaagat taattagggg ttcccacaac gacacaaaga cactatggat tgcccacaaa
     1621 ggcatagcga agcaaaggac gcggtagttt tcccacaacg acacaacgac acaacgacac
     1681 tatggattgc ccgcaacggc agcgaagcga aggacgcggc agttttccca caacgacaca
     1741 aagtcactat ggattgccgc aacggcacag tgaagcgaag agcacggttt aattcccgca
     1801 tggatacggt ggtggttgcc cacataggaa aaaagtatac agttattccg gcatcggaga
     1861 tgccagacct tattggtaaa aataagtacg gaacaatagc ttcggagaag ctcaaccatt
     1921 aaagtcccaa ttcataaatt aacgggggat aatatcattg atgggtgatg ttgggcttct
     1981 ccgaagccgg ggatttactt ttaatgagta ttaaaaacgt ggagcatctt cgatgctgga
     2041 ggtttttttc tgaggtttct cacaaagaca ctataggttg cccacaaagg cacaaaggca
     2101 caaaggcacg atttaatttc ctcaacggta acaggggtgg ttgcccacta agacacaggg
     2161 ttcacgaagg taggagttga aaatatctgc tccgcgtacc ctgtgacctc cgtgcgaccc
     2221 aaaccgccct caacctataa ggaagggcga ctctttaacg cctggcgcac cgcctgttct
     2281 atcgcatgga aattctggtt gtctgggtct ttttgaaaag ccttgcctgt gactttttca
     2341 tataattcaa tataccggtc ggaaatctcc cagacgaagg catcgggcat gtccggcatc
     2401 acctgtccgg ccttgccggc aaatccatgg gccatcaacc attcgcgcac aaattccttg
     2461 gacaattgtt tttggcgttc gccgctttcc tgcctttggg catatccctc gaggtaataa
     2521 tatctcgaac tgtccggggt atgcacctca tcgatgagga ggatctggtc gttgtagcgc
     2581 ccaaattcgt acttcgtatc caccaggatc aggccctggt cggcggccat ttcggtgccg
     2641 cggttgaata gggccagggc gtaccgctcc atggtaatcc attcttccag gctgcacaga
     2701 ccctgggcga tggcttgttc tttggatata tcctcatcat gtccttgttc ggccttggtg
     2761 ctcggagtga tgaggggctg tggaaaccgg tcggattctt ttaagccttc cggcatgtgt
     2821 acgccgcaaa tcattcgttt accgctttga tattccctcc aggcatgccc ggccagatag
     2881 cccctgacta cgaattccaa tttcaccggc ttgcattttt taccaatgga aacattgggg
     2941 tgggggacgg attccagcca gttgggcacc aggtcgcggg tggcctcgag aaaatgagcg
     3001 gccacctggt tgagcacctg ccccttgtat ggaatgaggc ggggcaggat atggtcaaaa
     3061 gccgagatcc ggtcactggc gatggatatc aggcggtcgc cgaccgtata gatttccctc
     3121 actttccctt tatacactcc ctgatcgggg gaaaaatgaa aatcggtatg gcctatgggt
     3181 gaataaaact cagacatggg gtaaatgtaa tacaagtccg gggcctgaag gtcgttgacc
     3241 gcgcgccagg atcagatatt ctgagtcaga ttccggtcaa acaggatgtc cttcaattct
     3301 ttcaacaagg cgtattcctg gggcagcact tttttgaacg cttctttggc agccaggtag
     3361 gagccttctt ccaactggag aaatttttcc tccaccattt tcagatcgtg tttgatcagg
     3421 ccgcggatgg aaggcatatc gtgccagtcc gcttcgatcg cgagattttt catgcggtcg
     3481 atttcatcgg cttccagctc gatcatttcg ccttcgcggt taaaaaaccg ctgggtctgg
     3541 tccttggcat gcccggaagg gagggtccat tgatcgtttt ctttgaccag gaaaaattcg
     3601 aggcccttat cgtggaaccg gtaaaagatg aaccggattt ttaccaatac atcgtgcata
     3661 aggataaagt taaataatta ttgtcgaaaa agaactttat tcattcatca agggataagc
     3721 ctccgggccc ggaaaggttc agaagtattt aaaattgtgg tttttttcaa tcttgccgag
     3781 gctgtggatc atcagttgga tgacattttc cacatcctgt ttgtgggcca tttccacggt
     3841 ggtatgcatg taccgcaggg ggatggatat cagtacggtg ggtaccccct ggttggaata
     3901 agcaaaggcg tcggtatccg ttccggttgc ccgggaggag gcctgccgct ggaaggggat
     3961 cccgttgacc atggcggcat cggtcaccag ccggagcagt ttctggtgta cggagggggc
     4021 atgggtgatc cccggcccgt cgccgcactt gacatctcca tcttttttct tgttcatatg
     4081 cggggtggag gtgtcgtggg tcacgtcgat cacgatggca gcattgggtt tgatccgccg
     4141 ggcgatcatt tcggctccat ggaggcccac ttcttcctgg accgcgttaa cgatatagag
     4201 ggagtagggg agctcaattt tattttcctt gatgcggcgc gccacttccg cgatgcaata
     4261 acctcccagg cggttgtcga gggcccggcc gacgtaataa ttttcattta aaacaaaaaa
     4321 gtcatcctca taggtcatga cgcatccgac atggatgccc agtttaccga cttcctcctt
     4381 gtccttggcg cctacgtcca caaaaaggtt ttctatggac aatttggggt cttccgcggt
     4441 cgaagaacgg gtgtgtatgg caggccagcc gaacacgcct ttcacgatcc ctttatccgt
     4501 aaaaatgttt acgcgtttgg atggggcaat ctggtggtct gaaccgccgt tacggatcac
     4561 gtggatgagg ccgttgtcgg tgatgtagtt gacgaaccag gagatttcat cggcatgggc
     4621 ctcgatgacc acccggtagt cctgcccggg attgatcacc gcaaaggcgg tcccgtaatc
     4681 atccagttcc caggaatcgg taaaaggctt gacgtattcg agccagagct gctggccctt
     4741 cacttcaaag ccggttggag agggattgtt gatataattt tcaagaaact gttcggagct
     4801 gagtggtgtc attcgaaaat ttaaattagg ttaaacggaa tgtttttctg accaggcttt
     4861 tgggtccttg ttccaggatt ggagcagatc aaaatcatcc cggctgatca cctcattgga
     4921 actggccacc cggagcaggg tggggtaatc ggtcagggtt tgagccttga cctctgcctc
     4981 tttaaaggcc ctggtgcagg cttcaaaacc ataggagaaa atagcaagca gaccggtaac
     5041 cactgccccc gcatcgcgaa gggcctggac cgctttgagg ctgctgccgc ccgtggacaa
     5101 cagatcttcg atcaccagga cccgctggcc tgcttccagc ctgccttcga tctggttgcc
     5161 gaggccgtgg cctttggctt ccgaccggac atagatcatg ggcaattcca tccggtccgc
     5221 aatcagggct ccataggcaa tccctccggt ggctaccccg gcaatgacgt caaaaggcac
     5281 aaaaggtctt gattcgcgga tgaaggcatc gaccacctgg ctccggaatt ccgggtagga
     5341 caggatgagc cggttgtcgc aataaatggg tgatttcagt ccggatgccc aggtaaaggg
     5401 tttttgagga ctaagtttaa ttgcttttat ttgcagcaac cgataggcaa tatgctcggg
     5461 ggtcaacatg aatctgaggg cgcaaaaata taaattctgc atcaatacca cctggatcct
     5521 gatcctggat aaaaaacggc tgcacaaact gagcatgaaa gattttccta tggccctggt
     5581 tttacaggag ccgggcatgg ccctgatccg ggaaaccatg catcaattgc tggacgatat
     5641 catccgcatg ccggtgatca tctccacccc ggatccgaag gcggtttggg aaaaaatcct
     5701 ggtcaagaac acactgatca aggcggcagg aggggtggta aaaaatccgc agggcaaatt
     5761 cctgctgatc caccggaggg gatggtggga ccttcccaaa ggaaaaatag acgctggtga
     5821 gactcctcgc aaagccgctt taagagaggt aaaggaagaa gtgggcctgg actgcaggat
     5881 cctttccgcc ttacccgata cctggcatat ttaccggctg aacggagagc tcatcctgaa
     5941 aaaaacctac tggtacctga tggcttcttc aaatagcagg acaaggcttc aagaagttga
     6001 agatataaca gggcataaat ggtttacaaa caataaattg attatgttta aaaataaggt
     6061 atatccgaac ctggcggact tgttgcaatt tgtggcgcaa tagtgccaaa gcctgcaagt
     6121 ggatgtcact tcgcgccgca ctgagcgcca gcatgtggca atcacttagc gaagcccgat
     6181 accctcataa acctggatct gggcaaaagc cccccatccc ggaacgagcc ccaaacccca
     6241 gcctgaaggc cggggtttgc ggcctgagcg ccagcatgtg gcaaagcggg ctgcaagtgg
     6301 ttgtcactta gcgcccgccc tgagcgcccg catgtggcaa tcacttagcg aagcccgaaa
     6361 ccccttaaaa acctggatct gggctaaagc ctccatcccg gagcgagccc ccaaacccca
     6421 gcctgaaggc cggggtttgc agcctgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnagc atgtggcaat cacttagcga agcccgatac cctcataaac ctggatctgg
     6601 gctaaagccc ccatcccgga gcgagccccc aaaccccagc ctgaaggccg gggtttgcag
     6661 cctgagcgcc agcctgtggc aaagcgggct gcaagtggtt gtcactttgc gcccgccctg
     6721 agcgccagca tgtggcaatc acttagcgaa gcccgatacc ctcataaacc tggatctggg
     6781 ctaaagcccc catcccggag cgagccccca aaccccagcc tgaaggccgg ggtttgcagc
     6841 ctgagcgcca gcctgtggca aagcgggctg caagtggttg tcactttgcg cccgccctga
     6901 gcgccagcat gtggcaatca cttagcgaag cccgataccc tcataaacct ggatctgggc
     6961 taaagccccc atcccggagc gagcccccaa accccagcct gaaggccggg gtttgcagcc
     7021 tgagtgcaag catgtggcaa agcgggctgc aagtggttgt cacttcgcgc ccgccctgag
     7081 cgcctgcatg tggcaatcac ttagcgccgc ccgataccct cataaacctg gatctgggct
     7141 aaagccccca ttccggagcg agcccccaaa ccccagcctg aaggccgggg tttgcagcct
     7201 gagctccagc atgtggtaaa gcgtgttgca agtggttgtc acttcgcgcc cgccctgagc
     7261 gtcagcatgt ggcaatcact tagcgaagcc cgatagctat ccgggaccca aactccgtga
     7321 cctccatacc tccgtgcgct tacctacgaa aaaccgatac tcagtgagca gaccgccaga
     7381 ctgcccggca tcgacggggt acataaatat atttctgtaa atctcccagg ggaaacctag
     7441 cgattcttgc caatctccct tgcaatcacc agtttctgga tctccgaagt gccttccccg
     7501 atcgtgcaga gcttggagtc cctgaaaaac ttctccaccg gaaagtcttt agtatatcca
     7561 tatcccccaa atatctggac ggcatcggtg gccacctgta cacagatctc cgaagcgtaa
     7621 tatttggcca tggccgcttc ccgggtcatt ttttccccgc tgtctttcag gctgccggct
     7681 ttgcgggtga gcagttcagc catctggatt ttggtttgca tatcggccaa tttaaagccg
     7741 atcgcctgga agtgggcgat ggcttggcca aactggtgcc tttctttggc atactgtacg
     7801 gaggcttcgt aggcgccttt ggcaatgccc aggctgagcg cggcaatcga aatgcgcccg
     7861 ccatcgagga ccccaagggc ttgcctgaaa ccgtctccgg gtttgcccag gacctgtgac
     7921 ttatgcaccc ggcattggtc aaagatcatt tcagcggttt ccgaagcacg catgcccaat
     7981 ttgttttctt ttttacctcc ggaaaatccg ggcgtccccc tttccacaat aaatgtggtc
     8041 atgccgttat tgtccagcaa ttcccccgtc cgggcaatga ccactgccac atcccccgat
     8101 ttaccgtgag tgatccagga tttggtaccc tggatgatat aatggtcccc atcctcccgg
     8161 gcgatgcatt gcatccggcc ggcatccgaa ccggtattgg gctcggtgag gccccaggcg
     8221 ccgatccatt cgccggtggc caggagcgga aggtattttt ccttttgttc ttcgttgcca
     8281 aactgaagga tatgtccggt acacagggag ttatgggctg ccaccgaaag cccgatgctc
     8341 ggatcgactt tggagatttc ttcgaggatg gtgatgtact cctggtaacc caggccggcg
     8401 ccctgatatt gctcgggaac caggacgccc ataaaaccca gggtacctaa ttctttgaac
     8461 aattcagcag gaaagtgctg actttcatcc cattccatcg atttgggttt aatgttccgg
     8521 agggcgaagt cgcgacaggt ctgctggatc agttgaaggt cggggatact gtgaacggaa
     8581 ggcataaata tgacggcaca atgatagtca atttccaaaa aaggctaaaa cctccctttt
     8641 gttcgttatc ccttcgattt taacacttga agcttccgat tcaactaact ttaaaggcct
     8701 tcggctcgta tagcctgata aaaccgataa tatgatgaac ttcagaacgt tatttttctt
     8761 gctttttgcg ggcctgcctg tccttgcagc ggctgacaac tacctggtca cggtctatgg
     8821 ccggttggta gatgggggtg gaaacgggat aggcaatgcg gtcatctaca tcagccaggc
     8881 gccgggatcg ccctttaccg ttaatgaaaa agtaagtacc cttccatccg gggaatttaa
     8941 atggccggtt tatatccccg acagcattcc gggcggcgtc ctgcttctgg gatttcagaa
     9001 ttgtgaagga aaggaaattt tcgcgcgctt cgaattttca aagaataaac cggtgataga
     9061 ggcgaaattg attttctgtg aacgtccggt tgggaaaccc tgtgcatctg aagtcattgc
     9121 aaaaaagatc aacgataccc ttgccattgc atgggtcagg gccaaaggaa tacctccttt
     9181 catgcaccaa tggaccaatg ggctgaccgg ggataccatc cggttcaacc cgcagcttaa
     9241 gatcaggatc tgcgtggtca ccaaggattc gaccggctgc aaatcggaag cttgtgtgcc
     9301 cggctttaac aactcctgtg gaatttatat cgaacaggat ggtcgtttcc tgattgccaa
     9361 atccaaagaa gaacgcatcg tgtccctgct ctggagcacc ggggcggatg gggaaaaaat
     9421 agagatcaaa gaacccggcg aatattgcgt caaagcggtg atggcctccg gttgcgaagc
     9481 caaagcctgc atcctcgtga aacccgtaaa tctggatccc tgtgcggccg aaatcgtggc
     9541 ggaacgcctg ggttccgaca accaggatag taccccgggc atccggctcc aggtcaaatc
     9601 cggcttcgat ttaaaataca tcctgtggag caccggggag actaccagga ccatcgtggt
     9661 ccggaaatct ggtgaatact gtgtctcggt cagcgacaac gtccgttgca aaatgtatat
     9721 ctgcaaaaaa gtggaattgg gtgatgactc ctgccgcgta tttatccata tggaaaagat
     9781 tccagtgacc tcgaatacgg acgccggaca caaggtcagg ctgaccgcta aaacgacctt
     9841 caaacccaat ctttattact ggaacaccgg ggataccacg ccttccatcc tggtggaaaa
     9901 aagcggagaa tactgcgtga ccgtcagcaa tggagtgaat tgcaagactg cctcctgcgt
     9961 taaggtggat ttgcccggcc cgaatatttc ccgctgcgag gcctccatcg tggtctatca
    10021 aaaaaccacg ggggaactcg atctgatcac caaagcggcc ggcaaagccc ccttcactta
    10081 ccaatggatc acaggttcga cggatgccaa cattacggtg aaaaattcgg ggaaatattg
    10141 tgtgaccatc aaggatgcgg ccggatgcac catgcaggct tgcgtggaag tattgtttga
    10201 aaaaccttca gggggaaacc tgtttccgga tgcagataag atcaagctgg gcgttcagcc
    10261 cctggccgaa ggagcccaga tccagctcaa tattcacccc aatcccacgg ccgaccgcct
    10321 ggtttaccag gtcaattcca aactgagcga agcagggcaa ttgtcggtca tgaatcttta
    10381 cggcaaaacc atttaccagg aaaaaatcga tgtacaggac ggactttctt acggacagct
    10441 ggacctgtcc ttcctgcctc ccggggtgta tatggtgaac atccaccagc agggatttat
    10501 ttcatccaaa aaactgatca ttgaacgata ataaaagtac aggtcaacgg aaacccgccc
    10561 atcaggaatg aggcgggttt ttgtcttttt tgcatgcaaa ttgatgaaga catattgaaa
    10621 ggatgcagga aaggggaccg ccggtcccag ttccgtttgt acgagctctg ttatccctat
    10681 ttgatgggag tggccctccg gtaccggaaa caaagggaag aggccgcctc cgccgtcaat
    10741 atggcttttt tgaagatcct gaatcacctg gaccgcttca gggaaaatca ttcgttcaaa
    10801 agctggatgc ggcaaatcat ggtcaatacc ctgatcgacg aattccgcag cagtgcccgg
    10861 ttttccagcg aaagcggcgc cgtggactat gaaaaggaag tggccgtcaa cggagtgcat
    10921 gtcgactaca acaatgctga aatcaacctg aatgtggaac aactggtgag ttatatccat
    10981 gaattgaacc cgctgacggc cagcgttttt aacatgtatg tacttgacgg cttcccgcac
    11041 aaggatattg cctccatgct ttccatttcc gaagcggctt ccaaatggca cctcttcacc
    11101 gccaggaaac aacttcagga aagggtggaa acggcctccg aaagaaatac caacccactt
    11161 ataccaaaaa ttaaagttaa cccagtatga aaaacgaacc ttggattgac cgggtttaca
    11221 aagaatcaac cgaaacctgg gatgtgccgg tgaacctgga tcactggcaa caggctgcat
    11281 cgctgatcga tcgccgggat aagaaaaaga aacgggcggt ctggatccgg atggccggac
    11341 tcagcggggc agccctgatc ctgcttttga tcctgatcct ttggaggaat ggtccttcag
    11401 aaagctcacc cgcagcggaa ccccagcctg caggaagccc cgcggctgag cccatggccc
    11461 aggcctccgg aacgacggat tccccggccc ctggattccc cgtccaggcc attggaccta
    11521 ttgccgaaaa cacttcggga caaggttcat cttccaaccc atcctctcaa gcctatttct
    11581 ccaataatca ggccccccgg accatcggat taaagaataa agaaaagatc cggcaggcca
    11641 aaccttatcc tgccgatcca agcctgtctt catccatccg gatcaacgct cccggcttat
    11701 ccacagaagc cgccaccccg gctacgccgg tcccgcaaag ggaaagcctc cctgaggaag
    11761 gtttggaccc gctggacttt atggcgatga cctcctccca taaaacattg ccggtgaccg
    11821 tcctggagat accggtggaa gtggattata aaccattccg gcgaaaggaa ttcggtctga
    11881 ggcttgacct cacccgccag gcggaaaacg gccgatcggc ctccatgagc atggcaggcc
    11941 tggaagtttt tgccaggaaa acccttggga ccaaaaccta ttgggacctg agcctgggat
    12001 acaattcttt ctttaattca tccctctatt ccgaggtcct cacgtcttat caattccagg
    12061 gttttggatc tacggtaaaa aactatggcg tcaagccgga gtggatacac tacctgtacc
    12121 tgcaaatggc cggtggcatg caattgaata agcaccgggc ctttgcaggc atccggcccc
    12181 agcttctatt gggagccatc ggtgaggtgg accagatccg ttttaaggat ggggctgcgg
    12241 tcagggacct ttcatcggcg gaaatttcca acctggccaa tggctggctg gataaaggct
    12301 cgctgcatca gctcgtattc aacgtgcacc tgggatatga ataccagttt caccccaaat
    12361 ttggtatagg tttgcaggcc agccgcttat tgaatgatct gtaccgtccg cttcccggtg
    12421 atattgccca gcatgccgga gctaaatgga atctgggcat gggcatttct tacaagctca
    12481 aatgatcatg aggcctttaa aatatatcgg attgatcctg accggtttcc tgagcgtgac
    12541 ctgcaataag attgaactcc cgttgaaccc ggcggaaacc ccgatttttg aaatgaaagc
    12601 gaattttgga aaccaggccg tcaatctggc cgcgggggtg gatggcctcg aactggatac
    12661 ccgctttatt tccgaaaatc tggatgtatt gatcttacag ggagaactaa aaaagaaaaa
    12721 ttgtaacccg ccctgtccca actctttgcg gctaagcctc aggcaaaatc gacaggactt
    12781 cctggggaat tcgggcatcg gcctcaaccc gggcagacga cccttttaca gggaggcccg
    12841 ggacagtttt atcgtacatg gtaagagcct gtcccaattg cccaattcgg gttcgggcag
    12901 gtatgatttt ggctggaccc tgaatgataa aaaggtggcg gacaaggaaa tcgtttcgtt
    12961 tcctttcaat cctcagataa aaaatacggt ttgtctttat gtgagtcatc ccgatgggag
    13021 caaggccagc cagtgccagg tgatcgatta ttcggtcaaa gacagtttcc ccggactgag
    13081 ggtgagtatc gagacgcagt ataaccaaag ccggggatgg accctcgtac ccaaagtaag
    13141 gggcctcggg ccgttcaaat ataaatggga caataaatcc gcggacgccg tgcaggaact
    13201 cgatcccaaa caagcgtcca gccattgcgt caccgtgcag gatgccaggg gcaatacagc
    13261 cagcgcctgc gtcgatttta atccggaagg aaaggttaaa tgccgggcgg attttgaact
    13321 gaccgctgat atctccaggc tgaatcaatg gacccagtac aataccacgg aaatcgtttt
    13381 tgtcaacgaa ggcggccaaa agtacctgag ttccttgatg cctcaacccg cctcttcagt
    13441 attcgagatc ctcgaatcgg ccccatttga agaaaatgcc caacaacagc cgacccgcaa
    13501 attgaagatc cattttgatc ttttgctctt cggacccgac cgcaccgcga tacccttcaa
    13561 gggcaccgga acgatcgctg tggcacatcc caaataatta aggttttgct tttaggatat
    13621 cgaaaagcaa ttcgggttcg ttggtggtga tgaaatcgaa agactggctg acgaaccgtt
    13681 ccaggtcttc cgctgaattc acggtccaga cattcagggc cagttggctt tttttcgctt
    13741 caaccaccca acccggattt ttattaaaaa cagaaaagtg atagtccagc ccagaaatgc
    13801 catcctgttt tagctctccg ggggagcgct ccccgttgag gtattgggtg gcagcctggg
    13861 gatccagggc aaggacggcc ctgaggatct ccaggtcaaa actgatatag gtgacccagg
    13921 gttccgcatg gagattttga atcatttcga ctacccgttt tgcaatcttc ctgccttttt
    13981 ccaccccggc cggagagggt ttgatttcca ggaccagccg ggtaccggga ttctctttca
    14041 tgccttccaa taaataattt tccagggtcg gcagggtttc cccgttggat aatctaatgg
    14101 cagccaattg tgaataattg gttttttcaa tgggcatgcc atggaattcg ggatcatggt
    14161 ttacgataag cacctcatcg ttggtcatcc ggatgtcaaa ttcgctgccc gtacaccgga
    14221 gccggatggc ttcccggagg cttgcaatgg aattttcggg aagattattt tttttccagg
    14281 cgccccggtg tgccaccacc gggttttcat gaaaccggat caagggttgc tgtaccattt
    14341 gggaaacgaa aagaaggaaa ataagcagca tgttttaata ttggattggg atgatggcaa
    14401 aggatggtca ttggtactta tccttccacc aggataagat cctttcctga atcttgtttt
    14461 cagtgggatt gtcctggggg gagtacaagc gggtccctga aacgggatcg ggtaaatatt
    14521 cctgggcggc gaaattttgt tcgaaagagt gcgaataaag atagtctttg ccgtaatcca
    14581 gggatttcat cagccgcgtc ggggcatttc ggagatgaag gggcaccggc agatccccgg
    14641 tctgccgcac gagccgggtg gcgtcttcaa tggccatata agctgaattg cttttggggg
    14701 aagtggccag gtaaatcacg gtttcggaca ggatgattcg ggcttccggc atgccgatat
    14761 tgtggatggc ttgaaaacag gcctgggcga tcaggagggc attcgggttg gcgagtccga
    14821 tgtcttcaga tgccgacacg atcaggcgcc gggcgatgaa ctcgatgtct tcacctcctt
    14881 ccagcatccg ggcgagccaa tagacggctc cattggggtc gctgccccgt actgatttga
    14941 tcagggcgga ggccatgtcg tagtgctgat cgcctccttt gtcgtagcgg gccaggttct
    15001 gctgtaccgc ctggctgacc gccaggttgg tgacctgtat atccccggcg ccggtatggg
    15061 cggaataaag gatatccagt atgttgtaca attttcgtcc gtctcctcct gccagatgga
    15121 tcagggcatc ggtctctgta aaaatccaat gccttgtttt cagatattca tcctgtacac
    15181 aggcatgacg ggccatttga agaagttccg tttccgaaag cgggttcagg acataaacct
    15241 ggcagcggga aagcaaggcg gggatgactt caaaggatgg gttttccgta gtggctccga
    15301 tcaggttgat gatgcctttt tccaccgcgt ggagcaggga atcctgttgc gcttttgaaa
    15361 accggtggat ctcatcaata aacaggatgg gattgggttt gtcaaaaaaa cggtgacgtt
    15421 gcgccttttc aatggtctcc cggacatctt tgacccctga ttcaatggcg ctcatcggga
    15481 aatacggcct ttgctgggtg gcggcgatca atcgagccag agttgtcttg cccgatcccg
    15541 gcggtcccca aagaatcatc gaaggagtat tgcccgactc aatcaacttc ctcaaaaccg
    15601 cctccgggcc catgaggtgg ctttgccctg caaattcatc aagggtccgg ggccgcattc
    15661 gttctgcaag ggggatcaag agtggtgggt ggtggatggt gggtggtgga tggtagtcag
    15721 tctcgccgct cgctgctcgc atctcgcatc agttctcatt cttttgtttt acgatccact
    15781 gctgaatgtt tgggcagttc aaaaacaagg ggtccacgcg gatataagtg gattcgatat
    15841 gggtctccag ccacttgacc agcaattcgc tttttttgga ttctttggcc agggcctgga
    15901 atttggcgta atcctgtttg aggtttgcct ggtggggatc cgtcctggaa ttcagcaaga
    15961 ggattttgta atatttctcg cccgtgggag caatcagctc aaccggggag gaaatgcctt
    16021 tgaccttcat ggtgtccagc gtgaaataaa cttccggttc caggtccctg atttcaaaaa
    16081 aggtgtctcc ggacctgggg ttggtgatcc gtccgtcgtt gctgtaactc tgttcctttt
    16141 tatggccgta ttgtttaacc gctttggaga aggtgatgga atcgttcagg atcatcatcc
    16201 tcaccttagc cagggtattt cgtgcgaggt ccaggtcttg tacttcaatc tcgggtttga
    16261 tcaggatatg ccgcgtgtgg atggtattcc cgcgcaattc cagaagttgg atcaggtggt
    16321 acccgaattc ggattccacg atctcggata cctgtcccgg ttcgaggttg taggccgcgg
    16381 cctcgtattc gggcacaaaa ctgccgcgct tggcccagcc caggtcgccg ccgttccgtg
    16441 ccgaaccggg gtcgtctgaa aaggtggctg ccagttcttc aaatttttct ccagccttca
    16501 ggcgggcaat gattttatcc atcttgtcct tcgccctctt tttggattcc tcattgggta
    16561 cggggtagag gacaatctcc gaaacttcca cttcactgct gaggtaaggc atgctgtcct
    16621 tgggaatgct ttcataaaac tggcgcactt cggatggggt gatgaggact tcctccatca
    16681 gttgggtcct cattcgttcg gccgtaagct gattgcggag atcttcacgg aaatcatccc
    16741 ggacttcgct ggtggttttg ccgtaataag cttcaaactg ggtgacatcg ttgcccatgc
    16801 tttccaggat ccgttcaatc cgggcatcga gttgtgattc cagttcttcc tccgagacaa
    16861 tgacgctgtc gacaaaagcc tggtgcacca ggagtttcga tatcatcatg ttttccaata
    16921 aaaaacattt gtcttccggc cgcatgggcc cctgcctgga ctcggtcatt ttaatctgtt
    16981 cagccaggtc tgaaagcagg atgacctcat ttcccacttc gcaaatgact ttgtcgatga
    17041 cctgtttttg ggcttccagg ccgaagccgg aaagcaggac ggccaggatc aaaaggaagc
    17101 ggggtgcggg gatggaccta ttgaatatgg attttgacat tgttttttct tagttctgtt
    17161 tcgtacaatt tggttttgaa ttcttcgacc aatttgtttt tccttttatt gagaataatc
    17221 agtttggctt tttccgcaac ataagagaaa ggcgcgtttt cccgggtgga gaccaattca
    17281 agcactttaa aaaaatactg aaactccgca tcactgatgc tcagcctttc attcctggac
    17341 cagtttcggg cgctgatccg gtccttggga aagagggctt cgatatccga aaaattgtgc
    17401 cacacgctgt cttccaggtg ataaataccg ttgtacttat cggccaggcc cgcgagcctt
    17461 tttttgttgt tgtttttttc caggtcatcc caccactccc tggccctcct catttcatca
    17521 atatttgcgg gaatttttac aaagtaacaa cgaataatgg aggtctccag ttgatactgg
    17581 tctttatttt tctcataaaa atcccggagt tcctgctcag aaaccatgga atccaggttt
    17641 tgttcgatca gtttttgctg gaaattgagg ccgatcaggg actctttata ctttttcagc
    17701 aattgttcga ttttcaggtc ggccgggaca tgtttttcgg cttcattcaa aaacacctgt
    17761 tcctgggtcc atcgtgaggt gaagttcgat attatccgcg tgctgtcctc gcgggtgcat
    17821 tcgggaggga agatgccctc gaggtcgctg aggtacagct tttttgaata aacggaagcc
    17881 agtaccgggt ccccggcgcc cggatcgggc gcaatgaact gacatgcagc tgtccatacg
    17941 gtcagtacta aaaaaaaatg taaatgcaga ggcctcatgc tcctttaacc tatttgtccc
    18001 cttatttatt tttttatgag cgattcgaat acaccctggt tcagggtgat tggaaattct
    18061 ttttccaaat cggtgatcca tgctttttcc aggaagtcct ggtaatctgc aatgacatag
    18121 cctcgggatt catccaggct cttcaactgg ggagggacta ttttttccac tttccgccag
    18181 gtcgcgatcc cgttttcctt gtcaatggag tagggggtca cctgtttggc cgcccagggg
    18241 acttcagcgg tccctgcctc cggtttttca gcaaaggttt tttcaaagcg gaccaatccg
    18301 ggatagcttt tttccagatt ttttgccagc ttatccactt ctttcttccg gctcatctca
    18361 tagatcgcct tgatcgtggc ctcatccttg gaagccacgg tatagtcggt gaccagggct
    18421 ctttcgcccc atttgtattt attcctgttt ttgttgaaaa actggtttag gccgagggtg
    18481 tcggatgagg ctttatccca gaccgattga cgggtgatct cgaaaagcag gatgccttct
    18541 tcgtattcac gcatgaggtt cctgaattca gggtaagttt ttgcgaggtg gtcctgttca
    18601 aactcaatgg ttttttccgt aacataagta ttgaatatgc cgggtacgac ctggctggcc
    18661 ggggtgcctg ccgcgtattg cagcctttcc cgggtattct gcttcagata atgcgcgaat
    18721 tcgcgggtgc cataggattt ggagctgagc gtaaacaggt ccagatcggg cagggaaggg
    18781 gaaacctccc aggaatagga agtgaagtcc ggcggaaggg actgaataaa cgtattcagg
    18841 tgttcctggt tgactttaaa cccgttggcc tgtttgattt tgtctatgac cctgttccgc
    18901 gcatacgcaa agcgtccatc cgcttcgacc tgggctttga ttttttcccg ggattcctcg
    18961 taaggcaatt ccgctttcct gctgattcgt ttgatgatgt gccagcccac cctggattct
    19021 atgggcatgg agatgtcccc gtcctgctca agctggaaga tggcatcttc aaatgatttt
    19081 tcaaactggt tgatccctac gaaaccgata taaccgtctt tggctttggt gctttcgtca
    19141 tgggaaaaag tccggaccag ttcagcaaaa tcagctccgt tcttcagcat tttgtaaatt
    19201 ttttcagccg tgggtttacc tccctggtca tgttctcttt ccgcaggttt tttaatcagg
    19261 atctgggcga tttccatttc acggcgggcc gggcggatgt catcgacccg gaggatatga
    19321 taaccggccg aactccttac cggcttggaa aacgcgccgc gctgcaggga ataaatggtg
    19381 ttttccacct cataaaaacc gtccgggagc atggaagtga catatccaag gtcgccgctg
    19441 tgattgatgt ttgaacggtc gtcggaattt tcctggacca cttttccgaa aggcaccccg
    19501 ccggtcagtt ccccgtaggc cttcatggcg cgtttccagg ccgcaagagt gtcctggggt
    19561 ggggcattgg gagagagctg gaaaaaaata tgttttacgg caatatcgtt tcgctggcgt
    19621 tcataaactt ctcgaatgag ctgttcggtg acttccttgt ccatgatata agagttggaa
    19681 acctgttgtt tataggtatt caactcttcc atgagttcag gtaaagtatc cagcctcatg
    19741 gccctggcct tggctacctt cagcttgaat tttttgtaca ggtcaaggta ctcctggagc
    19801 gatttgcggg aaaaatccgc atcctttgca ttgttttttc catagatata caagaattcg
    19861 gagaccccga tgccttgtcc gttgacggaa aacaggattt catcggcttg ctgacctgag
    19921 gcgaaaaccg tacaccacaa aataaatatt aaagtataac tggttcgttt tgagaacttc
    19981 atagggtaaa gcgttaacgc tgcaaaagta cacattcctg atttaaaaag ggagtttaat
    20041 cagggattgg acggaggcgg gtatggattt gaagaacgga gtatccggtt cgaagcccgt
    20101 ggccactggg gtcaaaatta ccggggagac cggtaaggac ttgaagggtt tcgcccagtt
    20161 tatccggggg tgaccttgga ttacgattct gtaataccct gaccggccgg tgagggcctg
    20221 gttaacggag tatggcgacg ggcatgttgg gattgtaatt cccttccatg accggattct
    20281 ggatattgcc ggtatatgtc ttaacctcct ggctgatgta attgaacaat tcctggacac
    20341 tcactatttt gttgcggtcc ccgtcggctt cgcctttaag cccccggatc aggtaatggc
    20401 tgaatatgcc ctgtctcaaa ccgctgtctt ccagggaaac ttcacggttc ttgcaggaga
    20461 ggatgaaggc ggccccccct ttttcggctt ccaggatcga ataaaattta ttaagcgaag
    20521 cggaaaaatc cgccttcgca tcggccatgc tgccggcgta gcacgcgtcg atcatgcaca
    20581 atttgtgttt ggcttccgat tcttccatga tctgattgat ttcttcgtgg aaaagcttgt
    20641 tgttatagcc gtcataatct atgggcagaa aggctccgtt gaccccgtgg cccgaataat
    20701 agagcatcac cacgtcattg gcgtccgctt tgccaaattg ttcctgcaag gcatcgatga
    20761 tcttctggcg ggaggcatcc tcatcgatca gcactttaat ctggtggtct gaaatcgcgc
    20821 ccccttccgg acttttgaga aaagcataaa tctggtaagc atcatcatcg gcgtatttga
    20881 gcaccggcat atgggcgtac cgggcaaccc caacgatgac tgcccaaacc cggacttcct
    20941 catccgtttc cgggcggata tccgttctgg gacggattgc ggggcggttt tcgagttgcc
    21001 tcaccggccg cccccggtcc caaacggcgg cgaatacctt cccgctcctg aaatacatga
    21061 tgccttctcc ctggggcgcc gtatcgtccc attggccctc ataccggtcc ccgttggcat
    21121 aatagacgat gccccggccg taggggttcc catccctgaa aggaccgacg tatttggaac
    21181 cgtccttgta gatataaaag cccaggccat acatgcagcg gatctcgttg cagttttgaa
    21241 ggttcggggt ggacaaggtg ggcagggccg cgtttgcgtt gacgttttca ggcaggtttt
    21301 cctgactggc agccaggctt tccccttcag ccagtaaccg gtccccggac cagtttccgg
    21361 aggttttttt cccattggcc agcaggaggc ttccctggcc ttcgcgcagg ttgtccctcc
    21421 attggccatc atagaccgaa ccgtccgaat agtggtaccg gccataacca ttccggcggc
    21481 cctcggtcaa taatccttca taggtttctc cattggcata ggtgtatttg cctttgccgt
    21541 cgggaaggtc cgatttccat tcgccggagt actgtccccc caggctgtat tgcatggtgc
    21601 ctgttccgtg aagtttggac cgcaaaaact ccccggtata ggtatccccg tttttaaaaa
    21661 ccatcttacc cctgccttcc cggtaatttt ggacccactc cccggtatag atgttcccgt
    21721 tgctcatcca gagtgtgcct ttaccatgga ttttggtgtt cacaaattcg ccggagtacc
    21781 tggctccgct gggatacaca taggtgcccg acccgttgtt gcagttcccc tggaggcact
    21841 gggtaaagga cctggggttt aaaacaaaaa acaaggagac gaggaggaat attctggcca
    21901 tgataaggac taaatacatt tgccggcatg cttattgcaa agccccgttt gtttttaaaa
    21961 tatttaactt cgccgaaaat tttattgaat gcgaatcttc tctttattga cggcaatggt
    22021 cttgatggcc ggtgcatgta aaaacctgcc gaccaatgaa acgctttcct cccaggccgg
    22081 cggcatcaaa tccttcgggg ccgagatcac cgaagacgga tttgtgaccc ttgatcaggc
    22141 cactgccatg ctgggagggg tagacagttt gccgcttaaa ctgagcggta cggtgaaaga
    22201 agtatgtcag aacaaaggct gttggatgaa tgtggtcgca gagagcgggg agagccaaac
    22261 tgtttttgtt aagttcaaag attatggatt ttttgtcccg aaagactgcg cgggaaaaaa
    22321 ggtggtcatg gaagggatcg cctaccggga agtcactccc gtagatgaat tgaggcatta
    22381 tgcggaagac aaaggggctt ctgccgaaga aattgccgct attaccgagc ccaaggaaga
    22441 gttgaaattc atggccagcg gggtcctttt gtacgaaaag gaatagaccg ggccggtctt
    22501 taaacttcac gttcggtcat gtatgaaata agctaaaggc ctcttgcaaa aacagagaat
    22561 agtagtatct ttgcgaagct aaaaaagcgg aagtagctca gttggtagag cacaaccttg
    22621 ccaaggttgg ggtcgcgagt tcgagtctcg ttttccgctc caaacaagag tcaaaagtta
    22681 atagtcaata gtcaagagtt aaactgttag ggagtgactc ttgactcttg actcttgatt
    22741 cttgactatt gactattgat taatgagcac ccaattccta tcatgtttaa tgatgaactc
    22801 cgtgtacgga cgtttaactt tgctgttgat gccataaaag tattgaggaa tgttcctgaa
    22861 aatcaggaat gaaggatttt aaaaagtcag ttgatcagat ccataagttc aatggcagcc
    22921 aatttcaggg cgtcatgcgt aagcagatct aaaaacgaat ggtattccaa aatctgcata
    22981 gtcattgaag aatccgatga aaccttattc tggattgaac tgatagaaaa actatatcca
    23041 tctaataagg aagctttaaa agaaataaag gcggaatcct tggaactggt taaaatattc
    23101 tctaaagcca ggggcaaact ggctcccaaa taatatcttt gtcattcaat actttagcaa
    23161 aggcctgttc taggaagtag aagaacttac tgactattga ctattgacta ttgactcttg
    23221 actcttgact attgactatt gactcttgac acgcccaggt ggtggaattg gtagacacgc
    23281 aggacttaaa atcctgtggc cagtaatggc cgtgcgggtt caagtcccgc cccgggtacg
    23341 aatttcagtg gtcagtggtc agtagtcagt agtcagttag caagttggca agtttgtaag
    23401 ttagccagta gttaatctcg atactcgctg ctggcttctc gctgctggct tctttagtcc
    23461 tgcctaactt gtatgacaag gaaatggatg gtcaggcggg ggctgtgtgt gctgtcgcca
    23521 aaagtattca gcaatcagat ctcagtggtc tgtaattgta tttttgctcc tttaaccctt
    23581 gacccttaac cgttcaccca tatttattga ggtcaatatt cagttaccaa gttggcaaag
    23641 tgggtttccc cctcaatcct caaccctcaa ttcctcaacc ctcaatcctc aattcctcaa
    23701 ccctaatttg cacatcagtc tgcaaaccct tggtccaggc ctgaaataaa cggctgttca
    23761 taaaaccgtt tttgggttgc cttgtagagg gcattggata gggcagcgaa cacaggaggg
    23821 aaaagaggct ccccgagacc ggtcggatcg gtttcatttt ccacaaaatg gatctcgatc
    23881 gattttggcg cctctacttg ccggatcatg cggtaggtgt caaaatttgt tttttggggt
    23941 accccatcct gaaaagtcat ctcgccgtat agggcatttc cgataccatc cacaatccct
    24001 ccttcaccca tattggccgc ggcgtcagga ttgactacaa ttccgcaatc caccgcggcg
    24061 taaacttttt gaatgaccgg gttcttgttt tggattacga gatccaggac ctcggcgacg
    24121 taggagttgt gacagaagta ggcggatacg ccccggtgga tattgggctg cggttcctgc
    24181 catttggatt tctccctgac cagttttaga accccggcat atcgatcggg atcatagtcg
    24241 ttgttttccc ctaccggatt ggttttggcc cggtccaata gttccagcct gaattcgata
    24301 ggatcttttc ccaattcttc tgccagttca tccaggaagg actgttcggc gcccgccata
    24361 aaattggagc ggggcgccct gaaggcgccg atggtgatat tggattcaat ggaccagctt
    24421 tccgccaggt aatggtcaat ggctccggca gggaagcggt ttgggtgaat gggggtctcc
    24481 gggattcctc cggctttcac atgaaaggcc aggagttggt ttttttcatc cagggccgcc
    24541 cggtaagtgg cggtataggt aggccggtat atgccgtagg tcatatcgtc ctcgcgggta
    24601 taaaccagtt tgacgggcgc cttcaacttt tgggagatca ccgcagcctc aaccatatgg
    24661 tggccataag ccctctggcc aaatcctcct cccatcctcg ccagtttgat atgtattttc
    24721 tccttcggca tccccagcct ggcaaccagg gtttgcatta tggattccgg cgcctggatg
    24781 gggccgtata tttccgcatg ttcatcagtg acccgggcaa aacaattgac cggctccatc
    24841 gtgttatggg caaggtaagg ggcggagtag gtcctctcga tcactttggc cgctctttta
    24901 aaagcggctt ccggatcacc gtctttacgg agcaccttcc cgggcttttt cgccatttca
    24961 gccatctggg ctttgtgccc tgccgtgctt tccaatccgg ccggaatccg gaccgtttgt
    25021 ttgcctcccc atccattgac cacaatatgg gaatcggata ttttttccca ttcgatgacc
    25081 aaagcctttt tggcattcat tacttcccag gtggtatttc ccgctacgac caccagttcc
    25141 gtgaaactgg tcgtatcgaa tccgtttctt tcatattcgt ccgcaagggt tttaatcgta
    25201 aaaacatcct tgatcccggg catagccctg gctgcggtgt catccaccga tttcactttc
    25261 attccgaatg cggggggatg ggcaatcatg gcgacgagca tcccgtcctg ggtatgatcc
    25321 aggctgaaca tgggcttgcc ggtcacaatc ttcaggccat ccacattttt cctggacgtc
    25381 ccgatgatct taaaatcctt gacctttttc aaggggactt cttcagggac gggaagggtg
    25441 gcggccaggg aagccaattc gccatatccc gctttttttc cgcttggcag gtgctgcagc
    25501 gtgccggctt cggtggtaat ctcttcgaca gggacgttcc aggtctgtgc ggccgctcgg
    25561 atcatcattt gacgagccgt cgcccccgcc gtgcgaagaa ttttccatcc ctggcgaatg
    25621 gcctggctgc cgccggtgaa ctggcgttca aaccgttcag gaaaaaaatc agcttgttcc
    25681 accctgacgt ttttccagtc tatatccaat tcttctgcca ggatcattgg catggaggtc
    25741 ttcacatttg atccgaattc cgggttggga gacatcaggg tgaccgcccc gttttcccca
    25801 atcttgatat agctgttcag ttcaaaccac tctttgggaa gggccaatat ttcttgggaa
    25861 gtgggtttac atccggctaa ccagttgaaa ctaagcatca ttcctccacc tgccagggaa
    25921 gtgactttga tgaaggatct cctgtttata gttgttttta ccgtggtcat tttatggaaa
    25981 agttaggggg attaggattt tgaggcggtt ttgactgcag ccctgatccg gagataggtg
    26041 ccgcaacggc agaggtttcc gttcatggcc gtttcaattt cttcatcgct tggatgcgga
    26101 ttatttttca aaaaagcgac tgcggtcatg atctgaccgg cctggcaata gccgcactgc
    26161 ggcacatcgt gttccagcca ggctttttgg accggatggt ccccgttttc cgaaagtcct
    26221 tcaatggtgg taatggcttt gttgccgacg gcggccaccg gaagcaggca tgaccttgtg
    26281 gcgaccccat cgagatggat ggtgcaggct ccgcactggg caatgccgca accatatttg
    26341 gtgccaacca ggttgagatg gtccctcagc acccaaagta caggtgttgc cggatcaagg
    26401 tccacttgcg tcttctttcc attgacattc agattaaata tggccatacg ggttatgttt
    26461 ttttagaata aataaaatta cggacaagtc cgcggtagca gtttactaaa ttcaaacgat
    26521 ttgttacaga attcaaagat cacgaagcaa agtcgtttgg agatgcccat gggctgagta
    26581 tcatgacatt ggcaaaataa acggttttcc tgctcattct ttcttcaatt gacctatccc
    26641 gccaagccgt ttccaatgaa aggttggtgg taatgccgtt tacccgttgc tttataaagt
    26701 gcattggcca aagctccaaa aacaggtggg aaggcgggtt cacccaaacc ggttgggtct
    26761 atatcgcttt tgacaaaatg cacatcaatg gctcctggcg cttcttccat tcggatcaac
    26821 cggtatttgt caaaattatt catctctggt tcaccctgcc ggaaggtcat ggatccatac
    26881 atcgcgttgc cgatgccgtc cacgatggca ccttcggtaa ggttgcgggc cgcgtccggg
    26941 ttcaccacga tgccacaatc cacgacacag gtgacttttg cgacttttgg tttgccttgc
    27001 tccatcacca ggtcgagcac ctgggcgaca tagctgttgt ggcaaaaata ggccgatacc
    27061 ccccggtaaa tccccggaat ttcttcctcc cagttggatt tttcttttag caattccaat
    27121 acccccgcat aacggtccgg gtcataatcg ttgtttttgc cgaccggatg gtttttggcc
    27181 ttttttaaaa gctcaagccg gaattcaatc ggatctttgc cggcggcctc cgccacctca
    27241 tcgaggaaag attgctcggc gacagccata aagttggacc ttggggcccg gaaagagcct
    27301 acggttatgt ttgattcaat ggcccaatcc tctgcgaggt aatggtccac cgctccggcg
    27361 ggaaaccggt cggcataaag cgggctttcc ggaattcctc cggctttaac ctggaaagcg
    27421 ataagattgt tttcggcatc cagtgcagcc cggtaatgcg catgataagc cgggcggtaa
    27481 atgcctgaag tcatatcgtc ttcccgggta tagatgagtt tgaccggcgc attcattttc
    27541 tgtgagatca gggcggcctc caccagccaa tgcgcatagg accttcttcc gtatccaccg
    27601 cccagtcggg tcatctggat atctatcttt tcaaggggca ggcccagccg ggccgacagg
    27661 gtcttttccg tatactccgg tttctgaagg ggtcctgcaa gttcagcttt atccgcagtc
    27721 acatgggcaa aaaagttcat cggttccatg cagttgtggg caaggaaggg ggcggtatag
    27781 gtacgttcaa tgacgcgggc ggggagtttg aaaactgcct ccggctctcc atcttttcga
    27841 cgcacctttc ctggtttagc cgcttccttc tccatttgca aaatatgttc tgaagagttt
    27901 tccaaaccgg cggggaaccg cacggtttgt tttccacccg gagtcaattt ggattccgtg
    27961 taagcggtga agagttccca ttctacgacc agggcttttt tggcattcag gacctcccag
    28021 gtggtctcgc ccaccaccgc gaccaggtcg gtaaaggtac aggtatcaaa gtattgcctt
    28081 tcatattcgt cttgcaaggc cttaatggta aaaatgtccc tgattccggg cattttcctt
    28141 gcagactggt catcaaacga tttcaatttc atcccgaagg cgggtggatg tacgatcatg
    28201 gcgatgagca ttccaggccg gtggtagtcc attccgaaaa gggcttgccc ggttacgatt
    28261 tttttcccgt ccacattctt acgggaggtg ccgaccagtt taaattcctt ggtatccttg
    28321 aggggcacct ccttcggcac tggaatcctg gcagctgcag ccgccatttc gccatatccc
    28381 gccgacctgc cgcttttttc gtgaagcaat accccttccg cggtagtgat ctccgtttcg
    28441 ggcacttgcc aggcttgggc cgccgcctgc ttcaacatat gcctggccgt ggcgccggcc
    28501 atccgaagac catgccagcc ctgccggatg gcctgactgc cgccaataaa ctggcgggta
    28561 tataatttcg tatccagggg ggcctgttcc accaccacct tctcccagtc cacatccagt
    28621 tcctccgcta cgatcatggg catagaggtt tttacattct gacccccttc cggattgggg
    28681 gacattatgg ttataaggcc gttatcgcct atcttcaaaa aaccgttgag gtcgaaccat
    28741 tccttgggca gggtcaaatc agcgttcgaa tcggactggc aggagggcag ccaactgaac
    28801 cctatcatca tgccgcctcc ggccaatgcg gtggctttca aaaatgaacg cctgccatta
    28861 aatgttttta caagggtcat gtttccggtt taaaggtgta aaatttactc cttactttaa
    28921 gttataaaat cggctgataa actccagacc aatcaatgat ttggtcttgt ttaaacccgg
    28981 gttttcacaa atttattctt gtattttgag attatgggtt tttgtttcca taatactcta
    29041 ttatcgatcg accattcgct ccgagctttc cgggtagcgg gctccggtga tcgtgatctg
    29101 agaagcagca tgttcaatct cacgcaggtc atcctgtgtc agttcgatcg aggtcgcccc
    29161 caggttttct tcaaggcgat tcaacttcgt tgtacccggt atgggcacaa tccatggttt
    29221 ctgtgcaagc agccaggcca gcgcgatttg tgcaggggtt gcattctttt ctttcgcgat
    29281 gtttccaagt aaatcaacca taccctgatt tgccttacgg gcttccggcg caaaacgggg
    29341 aactacattg cggaaatccg ttttgtcaaa caaggtatgc tcatcgattg ctcccgtaag
    29401 gaaacccttt ccaagcgggc taaaggtcac aaagccgatt ccgagttcct ccagggtggg
    29461 gattacctcc tcttcaggtt ttctccacca taacgaatat tcactctgaa gagcggttac
    29521 cgggaaaacg gcatgggccc tcctgatggt ttgaaggccc gcttccgaaa gtccgaagtg
    29581 cctgaccttt ccttcccgga tcagatctgc tacagcccca gccacctctt caatcggtac
    29641 cccggtatcc acgcggtgtt gataaaacaa atcgatcgca tctgtcttta atcttttcag
    29701 cgaggcttcg gcgacctgtt tgatgtgttc cggccggcta tccaattcat tccacttcgc
    29761 aggatcgctg gccgaaggtt tgaaaccaaa tttggttgcg atcttcacgg tcccgcgaaa
    29821 aggtgccagg gcttcgccaa ccagttcttc atttgtgaaa ggtccataca cttcagcggt
    29881 atcgaaaaaa gtgacccctt tttcaaccgc ggcgcgaagg agcgcgatca tttcccgctt
    29941 gttttggggt ggaccataac caaaactcat ccccatgcag cccaaaccga gtgccgaaac
    30001 ttccaggcca ttctttccaa gtcttcgtgt ttccatcgtc ttattcttta ttctagggtt
    30061 aacttttaaa tcaaatatat aggagtgccc gggccaggaa ataactaaaa tcaaattcaa
    30121 atactcctgt attttattgg gctcaacttt acatgttttt taaagaagtt gttgaaatga
    30181 gtgacttccc ggaatgccag ggcgtgagca atgtcggata ccggccaggc actgtgtttc
    30241 agcaggattt ttgcttcctg caacaaacgt ccggcaagga tctgcgtcgt ggttttcccc
    30301 gtcgtttctt tcacggaccg gtttaaatgg ttaacgtgaa cgtttaactg acgggcaaaa
    30361 tcggaggccg tgcgcaggtt gaccgccttg tggttatcgt caatgggaaa ttgtcgttcc
    30421 agcagttcca ggaacagggt ggaaattctt tgggaagcat tgagggcctg gtggtctatt
    30481 tttgcagaag gctgcatttt catggcaaaa tggatcagtt cgaataccat cgtcctcaag
    30541 gcgtcgtatt tataaatata gtccgaattg atctcttcca tcatccgctg gaatatgccg
    30601 ctcacctggt ttacctggcg gtcgcttaat tcaaagacat ggtttccgcc cggctggaat
    30661 aaagaatatt gattcaggtt gccgtagcgg tggaaaaaat gctggttaaa aatgcaaaat
    30721 acgccccggc ggatctggtc gatatgctcc catttatagg ggatttgagg atttgaaaac
    30781 gtcaaggctt gtttagccac ttcaaccacc tggtctgcat agtgaacctt gctcttgccc
    30841 accaccagca tgattttata aaagtccctg cgtttatagg gcaccggttg ggcatttttc
    30901 ccgacaaagg gatccagctt aaatacatta aaatgaccaa tctcttcccg aatagggtca
    30961 ggcatccagc cgatttttct tttataaaat tcctctagcg attcgacctt gtccatttta
    31021 ttagagttaa tcctgccgcg cgccgcggca ttcatttctt ttgcaaagta tggcccggac
    31081 ctgtttttta aaaacgaaga tcaaagcgtt cattacgaaa ttcaaaccgt taatcccagt
    31141 ccatgcattt ttcatacaca tagtctccgt catccagatt gtaggccacg aggtaaccca
    31201 aatctttcct ccgccgggag aacacacagc ctccgtccag attcctgcaa ttggccgtct
    31261 gagccaggat ctggccgagg ggcaaaggag tatggccgtg gagcatgaca cggtcaccga
    31321 gtgcggcttg attcgtgtcc tgaattcttt ggtaaagcat aaggtactca tctgaaaaga
    31381 ggtccggctc ttcaaaattg agacctgcat ggaccagaat atatttcccc gtttctgcat
    31441 aataaggcag gtcagtaaaa aaggctttgt aagggtccgg gaaagaggaa taggattcca
    31501 gtccgaaact ctctagcgtg gcatcgcccc cgttacggat ccagaattct aaaagtaact
    31561 cgtcgtgctc cgattccatc atcatttgct cgtggtttcc aagcagggca tggacccgga
    31621 tctggcgctc cttgaggttc agcagggtgt cgatcactcc tttgctgtcc ggtccccggt
    31681 ctatgtaatc acgcaggaaa aacaattcat cctttgaaga caccttcagt ttgttgaata
    31741 acagcccgtt tagcgttcta ttgcatccgt ggatgtctcc gatcacccat cgtctggacc
    31801 tggtcatgtg ctgaattgag gattttatgc agctctttaa ggtgaagtta agtgctttcg
    31861 gctgtccgga tcaatgaaac ccaccctcag gacaggctga ttgcgggatc gaacgttccc
    31921 cttatctttg caactcagaa attatgagca aacacggaag ggtactggta gccatgagcg
    31981 ggggcattga cagcacggtc gccgccatgc tgcttcatga acagggttat gaggtggtcg
    32041 gcattaccat gaaaacctgg gattatgcca acagcggcgg ggcaaaaaag gaaaccggct
    32101 gttgttccct ggattccatc aatgacgctc gtacggtggc cgtaaaaatg ggttttcacc
    32161 attttattct cgatatccgg aatgaattcg gggattccgt gatcgacaat tttgtggaag
    32221 aatacctggc cggacggacc ccgaacccct gcgtgctttg caatacccat atcaagtggg
    32281 aagccctgct caagcgggcc gatgccctgg actgtccatt catagccacc ggtcattatg
    32341 ccagggtcgg tgaactggaa ggcagaaaat atatttcaag ggcaaaagat acgagcaagg
    32401 accaatccta tgtgctggct gtgctgaccg cagaacagtt gcgccacgc
//
LOCUS       DAOQXT010000048        31805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1665_length_31805_cov_7.920472, whole genome shotgun sequence.
ACCESSION   DAOQXT010000048 DAOQXT010000000
VERSION     DAOQXT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 31805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31805
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_31805_cov_7.920472"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..123)
                     /locus_tag="PKM27_09955"
     tRNA            complement(50..123)
                     /locus_tag="PKM27_09955"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Ile,seq:cat)
     gene            complement(327..809)
                     /locus_tag="PKM27_09960"
     CDS             complement(327..809)
                     /locus_tag="PKM27_09960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR07626.1"
                     /translation="MIRRAVPSDLDDLLTLFKNTISFINSRDYNPQQTEAWISSIHDR
                     ERWLDKIQKQYFILYLEGGRVLGFASLDQGRHIDLLYVHHKRQGEGIAALLLLELETE
                     AKKYPGPPYLNTHASITALPFFTSKGFTIVRENKVNLKGVELHNYEMKKALDLNQEHE
                     "
     gene            852..1712
                     /locus_tag="PKM27_09965"
     CDS             852..1712
                     /locus_tag="PKM27_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR07627.1"
                     /translation="MDWKKIILSALVMITGPAVPAQKITRDIPYALTPEKELKLDLYL
                     PDGISNPYLVIWVHGGAWHSGSKENPPQDLVKLGYALASISYRRTSEAKFPAMIHDIK
                     AAVRFLRSHADQYGYRGDKITLLGSSAGGHLVALAGLTNKHEGLEGKIGNDLATSSEI
                     QAVIDFFGPADLTTILSQSTPHGLNVRTPALALMFGKPLEQATEELKMASPVYHVDAG
                     DPPLFICHGDLDPQVPINQSIQLYGKYRELDLPVQLEFVHGGGHGGKPFSDPALIQKV
                     DQFLKKHLGR"
     gene            complement(1761..2525)
                     /locus_tag="PKM27_09970"
     CDS             complement(1761..2525)
                     /locus_tag="PKM27_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNR07628.1"
                     /translation="MIRPYLLEETNWKQIKETKYDVVIQPWGATEAHNYHMPYATDNY
                     QVIHIAREAAGRAWEAGAKVLVLPNIPYGIQTGQLDIPYCMNLLPSTQLLIAKDLCDV
                     VLRAGCRKLVFVNGHGGNHFKNIIRELAFFYPSLFCCSLNWWEAAKTKEYFEDPGDHA
                     DEFETSAMMAIRPDLLLPLSEAGDGATKKFKVEALKKGWVQAQREWTKITKDTGSGNP
                     ALATPEKGKRYVEACISQIAGFLIDLAQVDVEEMYE"
     gene            complement(2676..3062)
                     /locus_tag="PKM27_09975"
     CDS             complement(2676..3062)
                     /locus_tag="PKM27_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNR07629.1"
                     /translation="MTENELAKIVVDICYRIHTELGPGLLESIYEAAFVYELEHLGIP
                     YTRQEIINVVYKGQVLGPAFKSDIILENKLIVELKSVERLENVHHKVVLNYMKLKNIK
                     LGFLINFNVALIKDGIHRKILGQLED"
     gene            complement(3232..4788)
                     /locus_tag="PKM27_09980"
     CDS             complement(3232..4788)
                     /locus_tag="PKM27_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNR07630.1"
                     /translation="MFVRTLTCAVHGVDAFPITVEVNAGGTVAAGSQYYFLVGLPDNA
                     VREGQQRIESALKNTGYKMPRMKFVINLAPANIRKEGSAFDLPIAMGILAGSMQIEAP
                     CLHEYLIVGELSLDGSLRPIKGALPISVLARKQQYKGLILPRANAREAAIIESVPVYG
                     INHLQEAIDIVNGRPSIETTKVDIRSEFNHRGNSYELDFADVRGQENIKRALELAAAG
                     GHNVILIGPPGAGKTMLAKRLPTILPPLSLEEALETTKIHSVAGLLAPGSSIVTNRPF
                     RAPHHTISDVALVGGGSNPAPGEISLAHHGVLFLDELPEFKRSVLEVLRQPMEERRVT
                     ISRARVSIDYPASFMLIASMNPCPCGYFNHPEKECVCGPGVVKRYLTRISGPLLDRID
                     LHVEVTPVSVQELSDYQSRGESSKSIVARVLSARQIQEKRFINYPGVYCNAQMDGKML
                     REVCSLKTDGVSLLRNAMERLQLSARAHDRILKVARTAADLEGSEAIQNQHLAEAIHY
                     RNLDREGWAS"
     gene            complement(4969..5367)
                     /gene="mscL"
                     /locus_tag="PKM27_09985"
     CDS             complement(4969..5367)
                     /gene="mscL"
                     /locus_tag="PKM27_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNR07631.1"
                     /translation="MLKEFRDFINKGNVLDLAVAVILGAAFGKIVGSMVEDILMPVIG
                     SIFSVNIADWKYVMKEAVLGADGSVTTAAVEFRIGKFIQTVIDFILVAFPVFLIIKAV
                     NKMKKAAPPEPAGPSTTDALLTEIRDLLKK"
     gene            complement(5458..6885)
                     /locus_tag="PKM27_09990"
     CDS             complement(5458..6885)
                     /locus_tag="PKM27_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNR07632.1"
                     /translation="MKWVILILLAVLDYSGHWTTSKQTLPNIVLIFFDDMGYGDLGCY
                     GALDVSTPHMDQLALEGIRFTNFLTAQAVCSASRAALLTGCYPNRIGISGALFPNAPN
                     GIRSAVMTLAEMLKQKNYRTGIFGKWHLGDKKEFLPLQHGFDEYFGLPYSNDMWPVHY
                     DGTPATDWKKDRFPPLPLMEGNEPVSFIHNHTDQGKLTRQYTERAVSFIEKNRRHPFF
                     LYLPHSMPHVPIAASSAFKGTSKQGSYGDVIQELDWSVGQVLKALKENHLEKNTLVIV
                     TSDNGPWLNYGNHAGSSGGFREGKGTSFEGGHRVPCIMRWQGKLPQGKVCNQLCSTLD
                     ILPTLASLSGCSLPTKKIDGIDVFSLLMNPESPSPREVFYYYYRRNALEAVRKGNWKL
                     VLPHPGRSYENQLPGRDGFPGAAPEDVPINPGLYDLRRDPAERYDVSAANPAVLAELT
                     ALAEEAREDLGDDLTGKKGKNVREN"
     gene            complement(6882..7967)
                     /locus_tag="PKM27_09995"
     CDS             complement(6882..7967)
                     /locus_tag="PKM27_09995"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNR07633.1"
                     /translation="MEPLKEMFNLVYYKKLVKVLSSVHSRLDARSLLNQLTAGMEERS
                     LNERLSHTSLVLGHFLPPDFKSALAILDKTIPAMDPGYTALVFPDFVARFGLDHFEAA
                     MDALKRYTVYGSSEFAVRHFLKKDFDKTIHRMKAWAFDANPHVRRLASEGSRPRLPWS
                     FKLDTVIKDPRSTTPILEALRADPELYVKKSVANHLNDISKDHPDYMLSLLNRWDRAN
                     SHTAWIARHASRSLVKKGYAPALRLFDAMEGAQVRLSRISLSPTTLPIGGSVRFQFYL
                     RSDSDKDQKLIIDYVVEYRKKMGGLSPKVFKLKEFILSKGKEILITKNHIMKDYTTRK
                     HYPGRHLLHIQVNGKIMASTSFHLTRS"
     gene            8016..8432
                     /locus_tag="PKM27_10000"
     CDS             8016..8432
                     /locus_tag="PKM27_10000"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNR07634.1"
                     /translation="MKKESRRKFIGNLGKASLAGGVMVKPAAAAKAGRELKKRFIHHV
                     YFWLKNPNSAADKAKLIEGLRKLSAVKTIQQFSIGQPADTRREVIESSYALSWFTFFK
                     NGKDQASYQVDPIHLKFVEDYSHLWSKVIVYDTVDA"
     gene            8541..9641
                     /locus_tag="PKM27_10005"
     CDS             8541..9641
                     /locus_tag="PKM27_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR07635.1"
                     /translation="MLDRREFLQKLAVGSGALAIPELHRGLFPADKILGIALVGLGNY
                     ATHQLAPALQHTQRCRLVGLVTGTPEKAKTWSAKYRIPEKNIYNYQNFDEIRNNPDID
                     IVYVVLPNSMHAEYTIRAAKAGKHVICEKPMALNVQECEAMIRACAENRVQLSIGYRL
                     YFEPHHLEMRRLGTQKILGNVKMMESSLGFSMANPKVWRLNKAMGGGGAIMDLGVYCI
                     QGARRTVGELPESVTAQGFVLDKSVFKDIYEGMTFQMNFPGGAVSNSTTTYSSYVDRL
                     YATTGGKWFELNPSYNATGAGGRFSDDQIRPFELKTKAFQQIDQMDDFADCIAAKRTS
                     SASGEEGLIDLKIIEAIKQSADTGRKVRLVWE"
     gene            complement(9644..10753)
                     /locus_tag="PKM27_10010"
     CDS             complement(9644..10753)
                     /locus_tag="PKM27_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07636.1"
                     /translation="MILFYLWWLAFYAIHPVPAAERPVHEFRDPHRYLYVAVPGIRNY
                     LEYGGHGLLVYDIDKDHKLVKRIPTRGLAPDGKPSNVKGIAVSLATQCIYISTVHNLQ
                     CISLQTEQILWEKAFEKGCDRLSVSPNGQEIYMPSFEKEDWYIIDAATGTLKHTISPG
                     SGAHNTIYGLNGKEVYLEGLRSPILTVVNAQTWTSRTVGPFSNNIRPFTVNGKQTLVY
                     ANINDFLGFEIGDLVNGNKLYSVKVNGFEKGPVKRHGCPSHGIALSPDERELWLADAH
                     NQQIHIFDNSQMPPQQKQSIALRDQPGWITFSKKGDYAYPSTGEIIDTRTKKILLTLH
                     DEHGIPVQSEKMVEVGFRGKEAVDAGDQFGLGRVQ"
     gene            complement(10759..10896)
                     /locus_tag="PKM27_10015"
     CDS             complement(10759..10896)
                     /locus_tag="PKM27_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07637.1"
                     /translation="MEAEEVEAEVEAEVEVEVEVKVKVEVECGSLKFRNSTILHPVPY
                     L"
     gene            10972..12285
                     /locus_tag="PKM27_10020"
     CDS             10972..12285
                     /locus_tag="PKM27_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR07638.1"
                     /translation="MHSSKHRRDFLKQAAALSSFFILPRHVLGRGFLAPSDVIQLGFL
                     GAGKQSETLMKRFLDTKQARILAIADPHRLKLDRFLGWHQKQYAEAEALKDQASNEVQ
                     TYENYLEVLHRKDIDAVAIITPDHWHAVMAIQAARVGKDIYGEKPLSLTVKEGRAMVD
                     AVRKNNRVFQTGSMQRSWPNFRKACELVRNGYIGTIKEVQVNIGNPPKAVDFESEPLP
                     DHLNWPLWLGPNDAHIYHHFLAPRLEDTFWAKWRDYIPFGGGGMTDWGAHMFDIAQWA
                     LDMDGSGPVRVIPPTTLPESGEIRGLEYHYENGVIMKHFNFGINNAVRFIGTTGQIDI
                     SRQVFNVPEKLKDLELKGSDLKLPAPENHYIDFFQSMRTRSKPICDVEIGHRTATVCN
                     IGNIAYQLRRPLEWNPKKELFKKDKEANALLTRKHWEQYAGLKNA"
     gene            12309..13121
                     /locus_tag="PKM27_10025"
     CDS             12309..13121
                     /locus_tag="PKM27_10025"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNR07639.1"
                     /translation="MKPSISKDLSDSFILTGQQLLFFKEHGFIKLKQVLSEETVNYMN
                     EVISSEVRRLNTQHLPMEERDTYGKAFLQIMNLWTQSPLVKEIVFSKKLAGLATALLG
                     TRGVKLYHDQALYKEPGGGHTPWHADQYYWPLDTDRCVTVWIPLQATPLDMGPLEFSA
                     GSQNLTTGRNKKISDESQALLESTLKEEGFPHIIEPFDLGEVSYHLGWLFHRAGANRT
                     DKMRSVMTIIYMDKDMKLKSPENENQELDWKTWCPGAKPGEVIETELNPELN"
     gene            13428..13895
                     /gene="tnpA"
                     /locus_tag="PKM27_10030"
     CDS             13428..13895
                     /gene="tnpA"
                     /locus_tag="PKM27_10030"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNR07640.1"
                     /translation="MANTYTQIHLHLVFAVQNRDGCIGKEWKEELYRYITGIIQNHGH
                     KVLAINGMSDHVQILIGMRPTQSLSDLMQDIKGDSSKWINQKDFLKSWFSWQSGYGAF
                     SLDKARVGTIIEYIKNQELHHLKKSFREEYLDLLKEFGVEFDIRLVFHEMEEV"
     gene            complement(14093..15661)
                     /locus_tag="PKM27_10035"
     CDS             complement(14093..15661)
                     /locus_tag="PKM27_10035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR07641.1"
                     /translation="MKKVSWIYWLGFFLLLKLLLHLRVNGTWSFHRDELLYLALGRHL
                     DWGYASVPPGIGFWAWFSSAVLGGSVEAVRLISTLFCSATVLLTALMAREMQPAGNPP
                     SKGIFSTLLIGLAGLLCGALLRPAMLFMPVVFDLFYWTLLCWLFLKYINTGRDRWILF
                     FAGFAALGFLNKYSVLIFLFALLPGLLLSRHRKLFTKPVLYYSILLGLLIVLPNLLWQ
                     YRHQFPVFRHLGELAATQFVHVSLSGFFSDQIMFFMPALPVWLAGLYFLLWRREAVSW
                     RIFGWMFLTVLAVLLLFKAKSYYSLGAYPVLLAAGAAFLEEQTRHGRAWLRPVLVIFM
                     LVLGLVTMPAILPVFKPEKEASYLKKLAGYPGLDGLLRWEDGRYYALPQDFADMMGWQ
                     EIADLAGKAWAQIPDKSNAAIYAENYGLAGGIEYFGKKYGIEQVLSFSDNYRYWLPDS
                     LPPDFHTLVYVNDELGDDMPGFFGKITLEGQLEMPLSRQHGVQVYICEKPTAAFFDRI
                     GTAIRNAKEEKEIE"
     gene            complement(15718..16044)
                     /locus_tag="PKM27_10040"
     CDS             complement(15718..16044)
                     /locus_tag="PKM27_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07642.1"
                     /translation="MGLIKFKQIKPIPFTMRTRYWDPVKEAREQRLKEIKAMQEDSIE
                     GMKTRLSRRFRGGYNEYYHAKKMAVRKQNLMLLVALIAVLYASYYILVNYLPRIEAWL
                     DHALTK"
     gene            16233..17042
                     /locus_tag="PKM27_10045"
     CDS             16233..17042
                     /locus_tag="PKM27_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07643.1"
                     /translation="MKSSVGIVFLLLFLHLLISSCDSLKKPGSRGKIPEVRDEVPNGG
                     ADAVYSMFIHTAIPDTLACGTEYYITVKANDIFIECENYNYGYLYLEARRLANARINE
                     LTCNLTACPFVHSNIVFHSGKCVNGFAQVELRVVVHCLRPNELPADGLEELVTPRMDT
                     AFLDSLPVPFEVRPHEDLFLQIKSDQAGKPCPKEYIYRINLIDDVISCQTVGNYGPYL
                     ERARNEATKIWLATRCGPDCSRRPWEELGASWTCEQNNVKIEYWFIVPCKK"
     gene            17439..19337
                     /locus_tag="PKM27_10050"
     CDS             17439..19337
                     /locus_tag="PKM27_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNR07644.1"
                     /translation="MSDSIKHECGLAVLRLKKPLEYYHEKYGTALYGLYRLQLLMQKM
                     RNRGQDGAGLVTIKLDAEPGKRYISRKRSNSVNYLTDLFHDVFNNFRGLKPSLLDDPH
                     WLKENMPYTGELLMGHLRYGTHGANTIETVHPFLRQNNWISRNLVLAGNFNLTNVEEL
                     FAELISFGQYPKEKSDTVTILEKIGHFLDDEVQRLFNWYKPDGYTNEEINQFIFENLD
                     IQRLLKRACKKFDGGYVMGGLIGHGDAFVIRDPNGIRPAYWYEDDEVVAMASERPAIQ
                     TALTVSVKYVKELLPGHALIIKRNGTVAEVPFVDQPAERKACSFERIYFSRGTDRDIY
                     LERKKLGEQLVEPVLKSVNYDFENTVFSYVPNTSESAFFGFIEGLQKNIDDYKRKSLL
                     DHKDRLTEQKINEIMNWRPRIEKLVVKDEKLRTFIADEEIRAEMVSYVYDVTYGIVRN
                     QVDTLVLIDDSIVRGTTLRNSILRNLARLKPKKIIIISSCPQIRYPDCYGIDMSKMSE
                     FVAFQALVELLKKNKKEYLLEETYQRCKMQEKLPKEEVVNEVQALYREFTPEEISEEI
                     ARIVKPADLDVEIEVIYQSLEGLHAACPNHTGDWYFSGNYPTPGGNKVANRAFTNYME
                     KINERAYA"
     gene            complement(19421..20953)
                     /locus_tag="PKM27_10055"
     CDS             complement(19421..20953)
                     /locus_tag="PKM27_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07645.1"
                     /translation="MRSGNRYEQGLWVFFFVALGLTSCRNKETTVVWNQGFPVIGSQS
                     SPRAADLNGDGTDDLVLGACKNEYQSTDYGVIALDGKSGEMIWHTDSRDQVYGSATLV
                     DVNGDSTLDVIIGGRWAYLRALNGRTGETFWNYSPPDTNDPVLKYARFNFFNSVLVPD
                     QSGDGLRDLLIQNGGNHAAKPQSFSDRFPGVLMLMDSRTGTILAADTMPDGQESYMSP
                     LYFEQPDGNQYIVFGSGGETASGSLYIGLLSDLKNKNLVHARAVATDTSHHGFIAPPV
                     LADLNKDGFLDLVAISHSSRIFAVDGKTMEPLWNRHIPGTESSNSLAVGQFTGDATPD
                     LFTVVSKGEWPNSRGSLQILLDGKDGSIAYLDSQGCAGFSSPVVYDLNGDGVEEAILS
                     INLYDCSRGYTAVSRLEIQNRLIAIDFKRRRIIPIDQAQNMKNIFSTPWIGDLDRDGY
                     LDIVHSQFFSPNYDLLVFLGMQIKRISTSIRYKQPAKWGGYMGSNGDGIYRDQGSVVN
                     SQ"
     gene            21091..22077
                     /locus_tag="PKM27_10060"
     CDS             21091..22077
                     /locus_tag="PKM27_10060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNR07646.1"
                     /translation="MNSKLKVFVSRKIPSLGPDLLRKEGFEVKIWPEERPMQQDELIQ
                     EAKQADILLSLGENKIDPYFLNECRHLKMISQFAVGYDNIHIPEATRLGIPVGNTPDV
                     LSEATADIAFGLMIAVSRKFFFNHKMILAGGWGSFKPTSHLGQELAGKTLGIFGLGSI
                     GMVMAQRCQGAFGMKVIYYNRTSNELAEKQFNARKVSFDELLAQSDVLSVHSVLSDET
                     RGLFDRQVFSKMKPTSIFINTARGGIHHEEDLYEALQNKVIWGAGLDVTNPEPMDKDN
                     PLLQMENVAILPHIGSATVEARDGMSKLAAENIISYIKSGVIPHCVNPAAIE"
     gene            complement(22162..23022)
                     /locus_tag="PKM27_10065"
     CDS             complement(22162..23022)
                     /locus_tag="PKM27_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNR07647.1"
                     /translation="MKLKLPIIVIMNLVVISGMFSQSLPAYQLFTGKGKKLKYAQLLK
                     RTAGKDLVLFGEEHNNPIAHWLQLRLTRDCSKQQPLVLGAEMIERDNQDELEAYLQGT
                     ITAKQLDSTARLWSNYKTDYAPLVNFAKQNKIPFIGTNIPRRFASLVAKNGFEALDTL
                     SDLEKSWIAPLPVKYDAELPGYKNMLTIMAGHGGPNLPKAQAIKDATMAHFILQNLNP
                     ETLFIHYHGSYHSENYEGILWYLLQEKPQLKYLTIATVSQTSLQRLDPGNLGKADVIL
                     AVDADMTKTY"
     gene            complement(23095..25446)
                     /locus_tag="PKM27_10070"
     CDS             complement(23095..25446)
                     /locus_tag="PKM27_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR07648.1"
                     /translation="MSRYNDLRRKPVVHMLRQITSILLLVLIPLIGQSQGCRLVIQGT
                     IVDESTGIPLEYAAVYLQETRQGVAADSLGQFMFQGICPGFYHLQISHIGCENQNLYL
                     RVDRDTSLFIHLHHHAELLNEIAVHGSREDNSTQMANSIARTEIARESNKNLADLLEK
                     IAGVSVIRNGSGISKPVIHGLHGNRITMINNGLVQAGQQWGNDHAPEIDPFLTDHLTV
                     VKGVSSLAYGGNGLGGVVLAESNPIEADPHLHGQLNYIFQSNGRGHTVNARLQQNRPW
                     AAWMLSGTFKRQGDQRAPDYFLTNTGKKENNLGIQFEKKFGSRWASRLYYSLFDTEIG
                     ILRGSHIGNLSDLEEAITRPVPFYTRDAFSYGIGSPRQAVVHHLLKLENKWLINEKNI
                     LSIHYGGQINQRREFDVRRSGRSDLPALSLRQYSHFVEGIYHVTPRPGVLIKSGLQYQ
                     YAYNTNQPGTGVLPLIPDYQALTGSAFGIYQRSFEKGQWEMGVRYDLRHLAAATISRE
                     LPRRIVRFNNNFNNINVSFGGKYQLNEVLSLTTNLGYVLRSPEVNELYSFGLHQGVSG
                     LEEGNPRLKQEKSFKWIVGLDGAVNHKFFIQALGYLQPIWDYIYLQPLNEFRLTIRGA
                     FPVYQYMQTHALLYGSDLLVSYEWNKSVKWTSRASLVIGQDQSRGLPLVYMPAQSLQS
                     DLTYSFGDRKRWSGTYLGAHGKFVFRQNRLEPGQDFLLPPYGYSLFSLEGGSSYAVGK
                     SRLKFSLLVDNLFNTPYRDYLNRLRYYADETGFNASFRLNYSF"
     gene            complement(25430..25987)
                     /locus_tag="PKM27_10075"
     CDS             complement(25430..25987)
                     /locus_tag="PKM27_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 periplasmic binding fold superfamily
                     protein"
                     /protein_id="HNR07649.1"
                     /translation="MFKLLPAYLLISALIFSHCRLDDPQIPNEEELITTLRYTLTPVN
                     GGNSAVFTFQDYDGEGGEDPLISGGVLTANTEYTGSLEILNEISFPLVDIGQEIQTED
                     TEHQFFLATSGGLLANVEYADTDSNGHPVGLITKLITRSVSSGNLTITLRHEPDKTAP
                     GVSKGDLSQAGGETDLEIVFDVQIQ"
     gene            complement(26105..26545)
                     /locus_tag="PKM27_10080"
     CDS             complement(26105..26545)
                     /locus_tag="PKM27_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07650.1"
                     /translation="MIRDDEEAPLNPQSSILSPHSSILSYPRDLNPFSPLSLQQQVRF
                     FRFISSISLAFLLALPPVLNTAHEWFHLQENHCHDTSSLHLHSVQHHCDLCDAFTAQV
                     FIAPSCKVQKNVRQEFQDKLPEYSGLAHRRTVSATLLRGPPGIC"
     gene            complement(26532..27122)
                     /locus_tag="PKM27_10085"
     CDS             complement(26532..27122)
                     /locus_tag="PKM27_10085"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNR07651.1"
                     /translation="MAKSIDKAAGGDPVANATATQLANIEKSTGKNLEEWTRIILSSG
                     LSKHGELVSLLKEKHGLTHGNANVLVHVAKQSHAGAEGNDTDWISEQYKGKENLKPWY
                     DKIMTAIGQFGTDIETAPKKTYVSLRRKKQFALIQPSTKDRLDVGLNIKGIAPAGLAT
                     AAGSWNSMCTHRIKVEDEKMINQELIDWLKKAYDQG"
     gene            27285..28202
                     /locus_tag="PKM27_10090"
     CDS             27285..28202
                     /locus_tag="PKM27_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNR07652.1"
                     /translation="MFRREFLKHTSLGTLALGAFPYGLTEDAKKTSRLGLQLYSVRDE
                     MKNNPVATVQALGKMGYRDCEHAGYNGQTRKYYGYSPAEWKKILGDAGLTMKSGHTTF
                     ASMHYNMESKQLTDAWKTVVEDAVLAGQEYVISPWLDISLRKNFDDLKRFMEGFNAAG
                     AYCKSQGIRYGYHNHDFEFSIKLTSQRIMDIIMENTDPGLVAQQLDIGNMYGGGGRAM
                     ELLKQYPGRFELMHVKDVIKASTDGLAHYESTVLGKGLIDVKKVLKYALRKGGTKYLI
                     IEQEAYQGMAPLDSMKEDLRIMKDWGYAG"
     gene            complement(28249..29142)
                     /locus_tag="PKM27_10095"
     CDS             complement(28249..29142)
                     /locus_tag="PKM27_10095"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNR07653.1"
                     /translation="MVSCRTPLEREPGSETKIIETSGAKLVKSGPLYGIRVSRDIPVK
                     NYFRTLDSIVSEYRSRVPYALSEHLLVHANPWIIDSLQTTDYYHQKQRGIFVYDQRQL
                     TVLHEGDSLFIPDSATAALIQNRLNQNWIDINIPEFSLRLYEKDQVIHCMNIRVGQNT
                     QRYQAVPGRFEDLRTVPGEGRIVRINREPTVFFDPHTGIKQTHTTRDDGKSTLMPLIP
                     WLEPEINGQRPGQLLHPTTNPKTLGKAYSNGCIAFSEGEAWLLYYHAPVGTRIVVRYD
                     LKIVNDGGDTVILPDIYNFFN"
     gene            29365..>31805
                     /locus_tag="PKM27_10100"
     CDS             29365..>31805
                     /locus_tag="PKM27_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNR07654.1"
                     /translation="MKPVLFFLFVFLACSAVAQKIPSPSEFLPSPYTKMFTHHAHLVD
                     YFKMVAEKSKQVKLVQYGETYEHRPLLLAFVSSEANIQRLEDLRVNNLRRTGLLPGNP
                     DKSDKTVFVWFSYSVHGNESAGSESSMQVLYDLVNPDHGDAREWLKNTVVVIDPSVNP
                     DGYSRYTQWYYNAANERVSPMRANREHREPWPGGRSNHYLFDLNRDWAWQTQAESRQR
                     LKIYNEWMPQVHTDIHEMGNNSPYYFAPAAKPYHQYITPFQASFQTEIGKNNARHFDQ
                     NGWLYFTREVFDLFYPSYGDTYPTYSGAIGMTYEQGGIGAGRAILTENGDTLTLSDRI
                     QHHRTASLSTIEVSSKNAAKLLDQFQEYFDRNNHNPQGRYKTYIVKGSNDPGKLRSFC
                     SLLDKNQIRYGRTDRQRKVTAYNYENGKEEPVSVESSDLLISTHQPKSVLLQVLLDPE
                     SHLEDSATYDITAWSLPHAYALETYATAERINPDKPFEPNGSKVVYAPSAYAYIATWN
                     SFEDARFLAALSDLGIRSRFATRDFKLEGKNFQRGSLIVTAADNRKTEAFHKIIQDAA
                     RTHHKSLTQVSTGFSDQGYDLGSSYMELARIPKVLVLGGEESSSLSYGATWYFFEENL
                     QYPVDLVDSRQLGSVDLREFNTIILPDGRFTISEKLKSWVSEGGNLIALGSALNAFAD
                     KEGFALKSYESEDAKKEAEKEKEKQDLDRRKFPLDQEERDGLMDFIPGAILKTSLDTS
                     HPMAFGLGRSYFTLKTEDLKFPLIKGGKNVIYLEKDWKQSGFIGSRLRDKLKETTVVS
                     VEAMGRGRIVYMVDNP"
BASE COUNT         8106 a         7831 c         8090 g         7778 t
ORIGIN      
        1 ccaaataaaa atggcctgat aaaattctgt ttatcaagcc attacaatgt gggcccacta
       61 ggacttgaac ctaggaccta ttgattatga gtcaagtgct ctaaccgact gagctatggg
      121 cccgtttgga gctgcaaata tagatcaaag ctggtgaaag aaagtggaaa atgaaaccga
      181 aggtgggttt atcaacacga ggaagaggag tccagagctg caagctcccg gagaatcctc
      241 ttccgtggaa ttctcgtcag ttattggact aaaagaggcg gcaggttatt gtcgacaaat
      301 tcacaaaatc cgacggaaga ccccctttat tcatgctcct ggttcaggtc caatgccttt
      361 ttcatctcat aattgtgcaa ttccaccccc tttaaattca ccttattttc ccggactatg
      421 gtgaaacctt tgcttgtgaa aaagggaagg gcggtgatgc tggcatgggt attcaaataa
      481 ggtggacccg ggtatttttt agcttcggtc tccaactcga ggagcaacaa tgcggcaatg
      541 ccttcaccct gccttttatg gtgcacatat aaaagatcga tatgccttcc ctggtccaag
      601 gaggcaaatc ccaatacccg gcccccttcc aggtatagaa taaaatattg tttttggatt
      661 ttatccagcc agcgctccct gtcgtggatg gatgaaatcc aggcttccgt ctgctgagga
      721 ttataatccc gggaattgat gaaggatatg gtgtttttaa acaaggtgag caagtcatcc
      781 agatcggatg ggacagcacg acggatcata gaggattata ttcaaaatta ttctaattat
      841 ttttatccaa gatggactgg aaaaaaataa tactgagcgc cctggtgatg atcaccggac
      901 cagccgtccc ggctcaaaag atcacccggg atatccctta cgccctgact ccggaaaaag
      961 aacttaaact ggacctatat ctcccggacg gcatttccaa tccttatctc gtcatctggg
     1021 tgcatggcgg agcctggcac agcggttcaa aagaaaaccc gcctcaggac ttggtaaagc
     1081 tgggttatgc cctggcgagc atcagttatc gccggacttc tgaggcaaaa tttccagcca
     1141 tgatccatga cataaaagcc gccgtccggt ttctccggtc gcatgccgat caatacggtt
     1201 accggggaga taaaatcacc cttttgggtt cctccgccgg tggtcacctg gttgccctgg
     1261 cagggctgac gaataagcac gaaggtctgg agggtaaaat cggaaatgac ctggctacaa
     1321 gttcggagat tcaggcagtg attgattttt ttggaccggc cgatttaaca accatactca
     1381 gccaatccac cccgcatgga ctgaatgtcc gcaccccggc gctggccctg atgttcggta
     1441 aaccactgga acaggcaacg gaagagctta aaatggcaag ccccgtttat catgtggatg
     1501 caggagaccc tcctttattt atctgtcatg gtgacctcga tccccaggta cccatcaatc
     1561 aatccatcca gttatacggc aaataccggg aactggattt accggtgcaa ttggaatttg
     1621 tccatggtgg cggccacgga ggcaagccct tcagcgatcc tgccctcatc caaaaagtgg
     1681 atcaattttt aaaaaagcac ctgggaaggt gagtttggct gatagctgat agctgaaaac
     1741 tgatatctga tagctgaccc tcactcatac atctcctcca catccacctg ggccaaatca
     1801 atcaaaaatc ctgcgatctg actgatacag gcctccacat accgttttcc tttttccggt
     1861 gtagccaggg caggatttcc agatcccgta tccttggtga tctttgtcca ttcccgctgc
     1921 gcctggaccc aacctttttt taaggcctcc accttgaatt ttttagtggc cccgtccccg
     1981 gcttcggaaa gcgggagcaa taaatccgga cggatggcca tcatggcgct ggtttcaaac
     2041 tcgtcggcat ggtccccggg atcttcgaag tattctttgg ttttggcggc ttcccaccag
     2101 ttgaggctgc aacaaaacag cgaaggatag aaaaaggcca gctcccggat gatgttttta
     2161 aaatgatttc ctccgtgccc gttgacgaaa accaatttac ggcatccggc ccgcaggacc
     2221 acatcgcaga ggtctttggc gatcagcaac tgagtgctgg gcagcaggtt catgcaatag
     2281 gggatgtcga gttgcccggt ctggatgcca taggggatat tgggcaggac cagcaccttg
     2341 gcccccgctt cccaggccct gcccgcggct tcccgggcga tatggatgac ctggtaattg
     2401 tccgtggcat agggcatgtg gtagttatgg gcttcggtgg cgccccacgg ctggatgacg
     2461 acatcgtatt tggtttcctt gatttgtttc caattggttt cctcgaggag gtatggtcgg
     2521 atcatattcg aaagatagat tttttttccc gctgagacac ggtaaggtgg cccacaaagg
     2581 cacggagaac acaaagagaa agtaaatatt tcgacaattc ctggctcatg ttatgctctt
     2641 ccaattacaa gctgtcaaat ttaatttcat cttgcttaat cctcaagttg tccaagaatt
     2701 ttgcgatgga tcccatcttt gatcaaggca acattaaagt ttatcaggaa cccaagcttg
     2761 atgtttttta gtttcatgta attcaataca accttatggt ggacattttc aagtctttca
     2821 acggatttca gctcgactat caatttattt tcaagaataa tatcggattt aaaagcgggg
     2881 cccagaacct gtcctttgta aactacatta atgatttcct gcctggtata cggaattcca
     2941 agatgttcca actcataaac gaaagctgct tcatagatgc tttccaatag gccgggtcca
     3001 agttcagtat gaatcctgta acagatgtcc acaacgatct tagccaattc attttctgtc
     3061 atattgagtt caatttggaa gatatgattt gattactaaa ttttcctatg ctcttgatgc
     3121 gatttggact tatcatattt ataaattaat ttgtgttctt ggtgtcttgg tgggagacct
     3181 taataaaaat tagcggtggg agcctttagt aaaaattcat tgtgggaaac ctcaggatgc
     3241 ccacccctcc cggtccaaat tcctgtaatg gatcgcctcc gcgagatgtt gattctggat
     3301 cgcctcgcta ccctccaaat cggccgccgt gcgggccacc ttcaggatcc ggtcgtgggc
     3361 ccgggccgat agctgcaatc gttccatggc attgcgcagc aagctaactc cgtcggtttt
     3421 cagggaacag acttcccgga gcattttacc gtccatctgg gcattgcaat agacccccgg
     3481 ataatttata aatcgttttt cctggatctg ccgggcggag aggacgcgtg ccacgatgga
     3541 tttgctggat tctccccggg attggtaatc cgacaattcc tgcactgaaa ccggggtgac
     3601 ttccacatgg aggtcgatgc ggtcgagcaa ggggccgctg atccgggtaa gatacctttt
     3661 cactaccccg ggaccgcata cacattcctt ttccggatga ttaaaatatc cgcaggggca
     3721 gggattcatg gaagcgatca acataaaact ggccgggtaa tcaatgctga cccgtgcccg
     3781 ggatatggtg actctgcgtt cttccatcgg ttgccggagg acttccagca ccgaacgttt
     3841 gaattcaggc aattcatcga ggaacaagac tccatgatgg gcaagagaga tctcgccagg
     3901 tgcgggattt gaacctcctc ccaccagggc gacgtcactg atggtatgat gaggggcgcg
     3961 gaaaggccgg ttggtgacga tgctggaccc gggagccagc agaccggcca cactgtggat
     4021 cttggtggtt tcaagcgcct cttcaaggct cagggggggc agtatggtag gcagtctctt
     4081 ggcgagcatg gtttttcctg cgccgggggg accgatcagg atgacattat gtcctccggc
     4141 ggcggccagt tcaagagcgc gtttaatgtt ttcctggccg cggacgtctg caaaatcaag
     4201 ttcataggaa ttgccccggt ggttgaattc ggatcggatg tccaccttcg tggtttcaat
     4261 ggaaggccgg ccattcacga tatcgatggc ttcctgaagg tggttgatgc catataccgg
     4321 aacggattca atgatggccg cttcccgcgc gttggccctg ggcaggatca gacctttgta
     4381 ttgctgtttg cgggcaagga cggaaatcgg cagggcgcct ttgatcggac gtaaactccc
     4441 gtcgagggac aattccccga cgatcaggta ttcatgcagg catggggcct cgatctgcat
     4501 ggatccggcg agtatgccca tggcaatggg gaggtcaaag gccgagcctt ctttccggat
     4561 attggcggga gccagattga tcacaaactt catccggggc attttatagc ccgtattttt
     4621 tagagcgctt tcaatccgtt gctgcccttc ccggacagcg ttgtccggca ggccgaccag
     4681 gaaataatac tgggagccgg ctgcaaccgt gccccccgcg ttgacttcta cggtgatagg
     4741 gaatgcgtcc acgccatgga ccgcacaggt aagtgtccgt acaaacatgt gttctcagtt
     4801 gtgttacata aagataggaa attcaccgat gaagcaatct ccccacggcc tggaacatac
     4861 tgactgattt tcaaataaaa agccattccg gaggaacggc tttttattta tttgcctttt
     4921 gaaaaataat tctaaacgcc taccaacctc aaccctcaac cctcaatcct acttcttcag
     4981 caaatccctg atttcggtca acaaggcatc tgtcgtagag ggcccggccg gttcaggggg
     5041 agcggccttt ttcattttgt tgacggcttt aataatgaga aagaccggga atgcgaccag
     5101 gataaaatca atgacggtct gtataaattt accgatcctg aattcaacgg ccgcagtcgt
     5161 taccgaacca tccgcgccaa gcaccgcttc tttcatcaca tatttccagt ctgcgatatt
     5221 gacactgaat atggatccga tcaccggcat gaggatatct tccaccatgg aaccgactat
     5281 ttttccgaat gcggcgccca ggatcaccgc aaccgcaaga tccaggacgt tccccttgtt
     5341 tataaaatcc ctgaattctt ttaacataaa ctatggattg gtgattttat aaatatactg
     5401 ctccgtcatc taatgtaata acaataaacc ataaaaaagc aaaaggaagg gcctccttca
     5461 gttctcccga acattttttc cttttttacc cgtcagatca tcacccagat cctctctcgc
     5521 ttcctcagcc agggcagtca attcggcaag aacagcagga tttgccgcac tgacatcgta
     5581 acgctccgca gggtctcgtc gaagatcata aagtcccggg ttgatgggta catcttcagg
     5641 ggcggcgccg gggaacccgt ccctgccggg caattgattt tcataggacc tgcccggatg
     5701 cggaagaacc agtttccaat tccctttccg cacggcttca agggcattgc gccggtaata
     5761 atagtagaag acttcgcgcg gggaagggct ttccggattc ataagcagcg aaaagacatc
     5821 gatgccgtca atcttttttg ttggaaggga gcaaccgctc agggaagcca gggtgggcaa
     5881 tatatccagg gtgctgcaaa gttggttgca gacctttccc tggggaagtt taccttgcca
     5941 gcgcataata cacggtaccc ggtggcctcc ttcaaaactg gttcccttgc cttcgcgaaa
     6001 tcccccggag gagccggcgt ggttaccata gttcagccat gggccattgt cgctggtcac
     6061 gatgacgagg gtattttttt caagatggtt ttccttcagc gcttttaaaa cctggcctac
     6121 ggaccaatcc agttcctgga tcacatcccc gtagctgccc tgtttgcttg tacccttgaa
     6181 tgcggatgag gcggcaatag gcacatgggg catgctgtgg ggcaagtaca ggaaaaaggg
     6241 atgcctgcgg tttttttcga taaaggatac ggcccgctcc gtgtattgtc tggtcagctt
     6301 gccttgatct gtatggttat ggatgaatga aacgggttca tttccttcca tcaggggcag
     6361 gggaggaaac cggtcctttt tccaatccgt ggccggtgta ccgtcgtaat ggaccggcca
     6421 catgtcattg gaataaggaa ggccaaaata ttcgtcaaaa ccatgttgta gcgggaggaa
     6481 ctccttttta tcacccaggt gccatttccc gaatatcccg gtgcggtagt ttttttgttt
     6541 cagcatctcg gccagggtca ttactgcgga ccggatcccg tttggcgcat tgggaaacaa
     6601 ggcccctgaa ataccaatcc ggttgggata gcagccggtg agcagggccg ccctggaagc
     6661 ggagcaaacc gcctgggcgg taagaaaatt ggtaaatcga attccttcca gggccagttg
     6721 atccatatgg ggggtggaca catccagggc cccgtaacaa cccaggtccc cgtaacccat
     6781 atcatcaaaa aaaatcagga caatatttgg cagggtttgt tttgaagttg tccaatgacc
     6841 ggaataatct aaaacggcca ataaaatcag gataacccat ttcatgatct ggtcagatga
     6901 aaagaggtgg aagccatgat ttttccattg acctggatgt gaagcaggtg ccttccggga
     6961 taatgttttc ttgtggtgta atccttcatg atatgatttt tagtaataag gatctccttt
     7021 ccttttgaaa ggatgaattc ctttaattta aaaactttgg gggacaagcc tcccatcttt
     7081 tttctgtatt ccacgacgta atcgatgatc agcttctggt ctttatccga atccgaccgg
     7141 aggtagaact ggaatcggac agatccaccg attgggagcg ttgtgggtga aagacttatc
     7201 cgggaaagcc tgacctgtgc cccttccatg gcgtcaaaaa gccgaagggc cggcgcatat
     7261 ccttttttaa ccaggctgcg gcttgcatgc ctggcgatcc aggctgtatg ggaattggcc
     7321 cggtcccatc ggttcaacag ggaaagcata taatccgggt ggtctttgga aatatcgttc
     7381 aggtggttgg ccaccgattt tttgacatat aattccggat cggccctaag ggcttccagg
     7441 atcggagtcg tgctccgcgg atccttaatg acggtatcca gtttgaagga ccagggcaat
     7501 ctgggcctgc tcccctcgga agccagtcgg cgaacatggg ggttggcatc gaaggcccag
     7561 gctttcatcc ggtggattgt tttatcaaaa tcttttttta aaaaatgtcg gactgcaaat
     7621 tccgaggatc cgtatacggt ataccgtttt aaggcatcca tggccgcttc gaaatggtcc
     7681 agcccgaaac gggctacaaa gtccgggaaa acgagagccg tatatccagg gtccatagcc
     7741 gggatggttt tgtccagaat ggctaacgcg gatttgaaat cagggggcag aaaatgtccc
     7801 aggaccaggg aagtatgact gagtctttcg ttcagggaac gctcttccat ccccgcggtg
     7861 agttgattca gcaaggaccg ggcatccaac cgggaatgaa cggaggacag gaccttaacc
     7921 agttttttat aataaacaag attgaacatt tctttgagcg gctccattcc aatctattta
     7981 aggggtatac ctatctttgc catcaaacca aaaatatgaa aaaagaaagc aggaggaaat
     8041 ttatcggtaa cctgggaaaa gcttccctgg caggcggggt tatggtcaag ccggccgcgg
     8101 ccgctaaagc aggccgggaa ttgaaaaaac ggtttatcca ccatgtatat ttttggttga
     8161 aaaaccccaa cagtgcagcg gataaagcca aactgatcga gggcttgcgc aaactttctg
     8221 cagtaaaaac catccagcag ttcagcatcg gccaaccggc cgatacccgt cgtgaagtca
     8281 tagaaagcag ttatgccctg tcctggttta cctttttcaa aaacgggaag gatcaggcca
     8341 gctaccaggt cgacccgatc catctcaagt ttgtcgagga ttacagccac ctttggtcaa
     8401 aggtgatcgt ttatgatacg gtggatgcct gagttaaggc taaggttaag gctaaggcta
     8461 aggctaaggt ggaacacctc aatctcaacc ttaacctcaa cctcagcctc aatcttagcc
     8521 tcaacctcaa tcccaacctc atgttagatc gaagagaatt ccttcaaaaa ctggcagttg
     8581 gttcaggagc gcttgcgatt cctgaattgc accgtggcct cttcccggca gataaaatcc
     8641 tgggtatcgc cctggtggga cttggaaatt acgcaaccca tcaactggct ccggctctgc
     8701 agcacaccca aagatgccgg ttggtcggcc ttgttacggg gacccctgaa aaagcaaaaa
     8761 cctggtcggc aaaataccgc attccggaaa agaacatcta taactatcag aattttgatg
     8821 agatccgtaa taacccggac attgatatcg tctacgtggt attgcccaat tccatgcatg
     8881 cggaatatac gatccgtgcg gcaaaggccg gcaaacacgt gatctgtgaa aaacccatgg
     8941 ccctgaatgt gcaggaatgc gaagccatga tccgggcctg tgcggaaaac cgggttcaat
     9001 tgtccattgg ttaccggctt tatttcgaac cgcatcacct ggaaatgcgg aggctgggca
     9061 cacagaaaat cctgggaaac gtaaaaatga tggaaagcag cctgggcttt tccatggcca
     9121 atccaaaagt ctggaggctc aataaggcca tgggcggggg cggggccatc atggacctgg
     9181 gcgtatattg tatccagggg gccaggagga ccgtgggcga actgcccgag tctgtgaccg
     9241 cccagggttt tgtactggat aaatcggtgt ttaaagatat ttatgaaggc atgacctttc
     9301 agatgaattt ccccggcgga gcggtttcaa attccaccac gacctattct tcctatgtgg
     9361 accgccttta cgcgacgacg ggcggcaaat ggtttgaatt gaatccatcc tacaatgcca
     9421 ccggggcagg cggaaggttt tcggatgatc aaatccggcc cttcgaactg aagaccaaag
     9481 cttttcagca aatcgatcag atggatgact tcgcggattg cattgctgcg aaacgaacct
     9541 cttccgcttc cggggaagag ggtttgattg atttgaagat catcgaagcg ataaaacagt
     9601 cggccgacac cggcaggaag gtcaggctgg tgtgggaatg atcctactgc acccttccca
     9661 atccgaactg gtccccggca tccaccgctt ccttgccccg gaaaccgact tccaccattt
     9721 tttcactttg tacagggatg ccgtgttcgt catgcagggt gagcaggatt tttttggtgc
     9781 gggtatcgat gatctcaccg gtggatggat aggcataatc ccctttttta ctgaaagtga
     9841 tccacccggg ctggtcccgc agggctatgc tttgtttttg ttgcgggggc atctggctgt
     9901 tgtcgaaaat atggatctgc tggttgtggg cgtcggccag ccataattcc ctttcatccg
     9961 ggctgagggc gatgccgtgg ctggggcagc catgcctttt cacaggccct ttttcaaatc
    10021 cgtttacctt gacgctgtac agtttattcc cgttcaccag gtctccgatt tcgaagccca
    10081 gaaaatcatt gatgttggca tagaccaggg tttgttttcc attgacggtg aaaggcctga
    10141 tgttgtttga aaaggggcct acggttcgac tggtccaggt ttgcgcattg actacggtca
    10201 gaattgggga ccggagacct tccaggtaaa cttcctttcc gttgagcccg tagatggtat
    10261 tgtgcgctcc cgagcccggg ctgatcgtat gttttagggt cccggtagcg gcatcgatga
    10321 tgtaccagtc ctctttttcg aaactgggca tataaatttc ctggccattg ggtgaaacgg
    10381 agagccggtc gcagcctttt tcaaaggcct tctcccaaag gatctgttcg gtttgcaggc
    10441 tgatgcattg aagattgtgc accgtactga tataaatgca ctgggtggcc aggctgacgg
    10501 caatgccctt gacattggag ggtttgccat cgggagccag gcccctggtg gggatgcgct
    10561 tgacaagttt atggtcttta tcgatatcgt aaaccagcaa gccatgcccc ccgtattcca
    10621 gatagttacg gatccccggt accgccacgt acaaataccg gtgcggatca cggaattcat
    10681 ggacggggcg ttcagccgca ggaaccggat gaatggcata aaaagccagc caccagaggt
    10741 aaaataaaat catgatattc aaaggtaagg aactggatgt agaattgttg aattgcggaa
    10801 ttttagggaa ccgcactcaa cctcaacctt aaccttaacc tcaacctcaa cctcaacctc
    10861 agcctcaacc tcagcctcaa cctcctcagc ctccatttac aatctcaatc attttcctta
    10921 atataggccc tctttttacc tcatttactg aatttgcaca atcgattgaa tatgcattca
    10981 tcaaaacacc gccgtgattt ccttaagcag gccgctgcat tatcctcttt ctttatcctg
    11041 ccgcgccatg tcctggggcg gggtttcctg gctcccagcg atgtcatcca gcttggtttt
    11101 ctcggggcag gcaaacaatc ggaaaccctg atgaaacgat tcctggatac caagcaggcc
    11161 cgcatcctgg ccattgcgga tcctcatagg cttaaattgg atcgattcct gggatggcat
    11221 caaaagcagt atgccgaagc agaagccttg aaggatcagg cttccaatga agtacagact
    11281 tatgaaaatt acctcgaagt gctgcaccgg aaagatatcg acgccgtagc gatcatcacc
    11341 ccggaccact ggcatgcggt catggctatt caggcagccc gggtgggcaa ggatatttat
    11401 ggggaaaaac ccctgtccct tactgtaaaa gagggaaggg ccatggtcga tgccgtaaga
    11461 aaaaacaacc gggtgttcca gaccgggagc atgcaacgat cctggccaaa tttcaggaag
    11521 gcctgtgaac tggtgcgcaa cgggtatatc ggcaccatta aagaagtgca ggtcaatatt
    11581 gggaatccac cgaaagcggt cgattttgag tccgaaccct tacccgacca tctcaactgg
    11641 cccctctggc taggaccaaa cgatgcccat atttatcacc attttctcgc gccgcgcctg
    11701 gaagacacct tctgggccaa atggagggat tatattccct tcgggggcgg gggcatgacg
    11761 gactggggag cccatatgtt tgacatcgcc caatgggcgc tggatatgga cggatcgggc
    11821 cccgtcaggg tgattccgcc gactaccctg ccggaaagcg gggaaatccg cggcctggaa
    11881 taccattatg aaaacggggt gatcatgaaa cattttaatt tcggtataaa caatgccgtc
    11941 cgctttatag gtacgaccgg ccagatcgac atcagcaggc aggtttttaa cgtccccgaa
    12001 aaattaaaag accttgaatt aaaaggttcg gacctcaaat tgccggctcc ggaaaatcat
    12061 tatatcgatt tcttccaatc catgcggacc cgctccaaac cgatctgcga tgtggaaatc
    12121 ggtcaccgca ccgccacggt ctgcaatatc ggaaacatcg cttaccagct gcgcaggcct
    12181 ctggaatgga acccgaaaaa agagctgttt aaaaaagaca aggaggccaa tgccctgctt
    12241 acccggaaac attgggagca atatgcgggc cttaagaacg cctgatcaaa aaacctattc
    12301 aaaccagcat gaagccatcc atatccaaag atctaagcga ctcttttatt ctcaccggac
    12361 aacaactgct cttttttaag gaacacggct ttatcaaact taagcaggtc ctaagcgaag
    12421 agacggtaaa ttatatgaat gaggtgatct cctccgaggt ccggcgcctc aatacccagc
    12481 accttcccat ggaagagcgg gacacctacg gaaaggcctt tcttcagatc atgaacctct
    12541 ggacccagtc ccctttggtt aaggagattg ttttcagcaa aaaactggcc ggtcttgcaa
    12601 cagccctgtt gggtacgcgt ggagtaaaac tataccacga ccaggccttg tacaaagaac
    12661 ccggtggcgg acatacgccc tggcatgcag accaatatta ctggcccctg gataccgacc
    12721 gttgcgtgac ggtgtggatc ccccttcagg ctaccccact ggacatgggc cccctggaat
    12781 tcagcgccgg cagccagaac ctgaccaccg ggcggaataa aaaaatcagc gatgaaagcc
    12841 aggccctcct ggaaagcacc ttaaaggaag aaggatttcc ccatatcatc gagccttttg
    12901 acctgggaga agtcagttat cacctcggct ggcttttcca ccgggccggg gccaaccgga
    12961 ccgataaaat gcgcagtgta atgaccatca tctacatgga caaggacatg aaattgaagt
    13021 cccccgaaaa tgaaaaccag gaactggatt ggaagacctg gtgcccggga gcaaagccag
    13081 gggaggtgat tgagacggag ttgaatccgg agttgaatta attaaggaat taaaggggaa
    13141 gggataaagc attttcatcg attagaatag gtctattttt tcgggattta gagcattatt
    13201 tgttctaata tccctttagg gatttcatgt gggtagcaat tttgaatgct catattgcgt
    13261 cccgtacggg acgcaacaac cacgtatcca catttctacc cacatgcttt gcctaaaggc
    13321 aaattattga aaactggttt tattttctct aaagcgaatt ttcaggtcat ttagtgccga
    13381 cttgccgggt tgattaaatt attcaggtaa ttttattcaa aattattatg gcgaacacgt
    13441 acacccaaat tcatttgcat ttggtttttg cagttcaaaa ccgggatggt tgtattggaa
    13501 aagaatggaa ggaagaatta tacaggtata taaccggtat tattcaaaat catggtcata
    13561 aggtattggc cataaatgga atgtcagatc atgtacaaat tctgatcgga atgagaccta
    13621 cccaatcctt gtcggacctc atgcaggata tcaagggtga ttcttcaaaa tggattaacc
    13681 aaaaagattt tttaaaatcc tggttttcct ggcaaagtgg ctacggggca ttttcccttg
    13741 acaaagcaag ggtgggtacg atcattgaat acattaaaaa ccaggagcta catcatctaa
    13801 aaaaatcctt cagggaagaa tatcttgatt tattaaaaga atttggggtt gaatttgata
    13861 taagattagt atttcatgag atggaagaag tataataaaa ttaaaatgta catagggcta
    13921 aaagacaata aaaggtaggt ttaagaatcc tttttattgc agacttcgaa gatgtgttct
    13981 gttttttctc ccggttttta ttgtaattta tcctactgcc cactggcctc tcgcaaagcc
    14041 tcggacctct gacgaacttg ctaacttgct aactgattaa ccagccaact acctactcta
    14101 tctccttctc ctccttcgca ttccggatcg ccgtaccgat gcgatcaaaa aaagcagcgg
    14161 taggtttttc acagatatat acttgtacgc catgctgacg ggaaaggggc atctccaatt
    14221 gaccctcaag tgtgattttt ccaaaaaaac cgggcatatc atcccccaat tcatcgttga
    14281 cataaaccag ggtatggaaa tcgggcggca aggaatccgg cagccaatac cggtagttgt
    14341 cgctgaaact gagaacttgt tcaatcccgt atttttttcc gaaatattcg atccctccgg
    14401 ccaatccgta attttcggca taaatggcgg cattcgattt gtccggtatt tgagcccagg
    14461 cctttcccgc aaggtctgcg atttcctgcc agcccatcat atccgcaaaa tcctggggta
    14521 gtgcataata ccggccgtct tcccacctca gcaagccgtc caggccggga tagcctgcca
    14581 gttttttcag gtaggaggcc tccttttcag gtttaaacac aggcaggatg gcgggcatcg
    14641 tgaccaatcc caggaccagc ataaagatca caagaaccgg gcggagccag gccctgccat
    14701 ggcgcgtttg ttcttcaaga aaagcggcac cggcggccag caggacggga taagcaccca
    14761 gggaataata acttttggcc ttaaataaca agaggacggc caggacggtc aaaaacatcc
    14821 atccgaagat tctccaggat accgcttccc tgcgccataa taaaaaatac aaaccggcca
    14881 gccagaccgg caaagcgggc ataaaaaaca tgatctggtc tgaaaaaaaa ccgctgagcg
    14941 atacatgtac aaattgagtc gcggccagtt cacccaggtg ccggaaaacc ggaaactgat
    15001 gccggtactg ccacaggagg ttggggagca caatcaacag gcccagcagg atggaataat
    15061 aaaggaccgg ttttgtaaaa agttttcggt gccgggacag gagcaaaccg ggcaggaggg
    15121 caaacaggaa gatgagcacg gaatatttat tcaaaaagcc cagggccgca aagccggcaa
    15181 aaaacaggat ccaccggtcc cggcccgtat tgatgtattt aaggaaaagc cagcatagaa
    15241 gggtccagta aaaaaggtcg aagaccaccg gcataaacaa catggccggc ctcagcaggg
    15301 cgccacacag caaaccggcc aggccaatca ggagggtact gaaaatgcct ttggaaggag
    15361 gattgccggc gggctgcatt tcacgggcca tcaaggcggt aagcaatacc gtggctgaac
    15421 agaataaagt ggaaatcaaa cgcaccgcct ccacggaccc gcccaggaca gcggaggaaa
    15481 accaggccca gaaaccaatc ccaggaggca ccgaagcata tccccagtcg aggtgacgac
    15541 cgagggcgag atacaataat tcatcccggt ggaaggacca cgtcccattc acccggagat
    15601 gcagcagcag tttgaggaga agaaaaaacc cgagccaata gatccaggaa acctttttca
    15661 tatttatggt taaaatagat attatggtcg gaaaaatcga aaaggttcct tgcccggcta
    15721 tttagtcagg gcgtggtcta accaggcttc gatcctgggg agataattca ccaggatata
    15781 ataggacgca tatagaacag cgatcagggc gactagaagc atcaggtttt gtttccggac
    15841 cgccattttt ttagcgtggt aatattcgtt atagcctccc cggaaacgcc tggacaaacg
    15901 agttttcatg ccctcgatgc tgtcctcctg catcgctttg atttccttga gccgctgttc
    15961 cctggcctct ttcaccgggt cccaataacg ggtcctcatg gtgaaaggga ttggtttgat
    16021 ctgtttgaat tttatcaggc ccatgctgta atttcaaaga taagggaatc ggtataaaaa
    16081 cgggctgggg tttttgggaa ttactctttt tgatatatat ttaaatattc atctttcctc
    16141 aagcgttttg ccgggcgaag cagtcatgaa tcaaagtgcc gttctgccgc attcatgacc
    16201 gaggggtcgg acaccttcta aaacattgcc ctatgaaatc ttctgttggt atcgtgtttc
    16261 ttcttctgtt tttacatctt ctcatttcct cctgtgattc cctgaaaaaa ccaggcagcc
    16321 ggggaaaaat accggaagtc agggacgagg tccccaatgg gggtgccgat gccgtctatt
    16381 ccatgttcat ccacaccgcc atccctgata ccctggcatg cggtacagaa tattatataa
    16441 cggtgaaggc caatgatatt ttcatcgagt gtgaaaacta caattacggt tatctgtacc
    16501 tggaggcccg gcgcttggcc aatgcaagga tcaacgaact gacctgtaat ttaacggcct
    16561 gcccttttgt tcactccaat attgtttttc acagtggaaa gtgcgtcaat ggatttgccc
    16621 aggtcgaact ccgggtcgtc gtccactgtc tcaggcccaa tgaattacct gccgatggat
    16681 tggaagaatt ggtgactccc aggatggaca cggcattcct ggacagcttg ccggtgcctt
    16741 tcgaggtccg gcctcatgag gacttgtttt tacagattaa gtccgatcaa gccggcaaac
    16801 cctgccccaa agaatatatc taccggatca acctgatcga tgatgtgatt tcctgccaga
    16861 cggtcggaaa ctatggtcct tacctggaaa gggcgagaaa tgaagcgacc aaaatctggc
    16921 tggccacccg ttgtggcccg gactgctcga ggagaccctg ggaagaactg ggggccagct
    16981 ggacctgtga gcagaataat gtgaaaattg aatattggtt tatagtgcca tgtaaaaagt
    17041 aaaattcctg gcgtagtaac caccggcgag tgcatagggc atttaccctt ggattttagc
    17101 ctgaatggcc tgtgatgatg gtttgaatat tatttaatct ccttaaacaa tgtgtaaagg
    17161 gtctggccgg gtccgctagc agccatggcc ggattcctcc ggaatccaat aagcctttat
    17221 gtatttgaat atcatttgtt tataaggcgc ttgaacagtc caaactacca cccgggtatt
    17281 taaatgcgaa atcaagtagg ttaaaatttt aatagagggg ttttacggca aacgtttttt
    17341 aagttaatct tttgcaaacg aaagcgggta aaataaaacc ggaaagtcgg catatcgcca
    17401 atatatatct ttaactttgc agtctccaac ccgggctaat gagtgactcg atcaaacacg
    17461 aatgcggcct cgccgtcctt cgactcaaaa aaccactcga atattaccat gaaaagtatg
    17521 gtacggccct gtatggcctt taccggttac agcttctcat gcagaagatg cgcaaccggg
    17581 gacaggatgg ggcaggcctc gtaaccatca agctggatgc cgaacccgga aaaagataca
    17641 tcagccggaa aagaagcaac agcgtcaact acctgaccga ccttttccat gacgtgttca
    17701 acaatttccg gggacttaaa ccctccctgc tcgacgatcc gcattggctc aaggaaaaca
    17761 tgccctatac cggtgaactg ctcatgggcc atcttcgtta cggcacccat ggagccaata
    17821 ccatcgaaac ggttcatccc tttctgcgtc aaaacaactg gatcagccgc aacctggtgc
    17881 tcgcaggcaa tttcaacctg accaatgtgg aagaattgtt tgccgaactg atctcttttg
    17941 gccagtaccc caaggaaaaa tcagacacgg tgaccatcct ggagaaaatc ggccatttcc
    18001 tggacgatga ggtgcaacgc ctgtttaact ggtacaaacc ggacgggtac accaatgagg
    18061 agatcaacca gttcattttt gaaaatctgg atatccagcg gctgctgaag cgagcctgta
    18121 aaaaattcga cgggggttat gtcatgggag gcctcattgg tcacggggat gcctttgtaa
    18181 tccgggaccc caacggcatc cggccggcat actggtatga agacgatgag gtcgtagcga
    18241 tggcatccga aaggccggcc atccagactg cgctcacggt atcggtcaaa tacgtcaaag
    18301 aacttctgcc tgggcatgcc ctgatcatta aaaggaacgg caccgtggcg gaagttccct
    18361 ttgtcgatca accggccgaa cgcaaagcct gttcctttga gcgcatttat ttttcaaggg
    18421 gcacagaccg cgatatttat cttgaacgga aaaagctcgg agaacaactg gtcgaaccgg
    18481 ttttgaaatc ggtcaattac gattttgaga ataccgtttt ttcatatgtt cccaatacct
    18541 cggaatccgc ttttttcgga tttatcgaag gcttgcagaa aaacatcgac gactataaaa
    18601 gaaagagcct gctcgaccat aaagaccggc tcacggaaca aaagatcaat gagatcatga
    18661 attggcggcc aaggatagaa aaactggtgg tcaaggacga aaagttgcgc acctttattg
    18721 ccgatgagga gatccgcgcc gagatggtct cttacgtgta cgatgtgacc tacggcatcg
    18781 tccgcaacca ggtggacacc ctggtgctga tagacgacag cattgtccgg ggtaccaccc
    18841 tgcgcaacag catcctgcgt aacctggccc ggctgaaacc gaaaaaaatc atcatcatct
    18901 cttcctgccc gcagatccgg tatcccgatt gttatggcat cgatatgtcc aaaatgtcgg
    18961 aattcgttgc cttccaggcg ctggtggaac tgttgaaaaa aaataaaaaa gaatacctgc
    19021 tggaggagac ctaccagaga tgcaaaatgc aggaaaaact gcccaaggaa gaagttgtca
    19081 atgaagtgca ggcgctctac cgcgaattta cacccgaaga aatctccgaa gaaatcgccc
    19141 gcattgtcaa gccggccgac ctggacgtgg aaatagaagt catctatcaa agccttgaag
    19201 gccttcatgc ggcctgcccg aaccataccg gtgactggta tttctcagga aattacccga
    19261 cgccgggtgg caataaagtc gctaaccgcg cttttaccaa ttatatggag aagatcaatg
    19321 aaagggctta tgcctgaatg agggttgagg agtgagggtt gaggagtgag gagtgaggag
    19381 tgaggaatgt gattcctgac tactgactat tgactattga ctattgacta ttgactactg
    19441 atccctgatc cctataaatt ccatccccat ttgatcccat atatccgccc cattttgccg
    19501 gttgtttgta ccggatggaa gtggatattc gtttgatctg cattcccagg aagaccagga
    19561 ggtcgtaatt ggggctgaaa aactgggaat gaacgatatc caggtagcca tcgcggtcga
    19621 ggtctccaat ccagggggtc gaaaaaatat ttttcatgtt ttgggcctga tcgatcggaa
    19681 tgatgcgcct ccgtttaaaa tcaatagcga tgagccggtt ctgaatttcc agcctggata
    19741 cggcagtata acccctggag caatcgtaaa gattaatgct gagaatggcc tcctcgacgc
    19801 catctccgtt gaggtcatat accacggggg aagaaaatcc cgcgcatccc tgtgaatcca
    19861 ggtaagcaat gcttccgtcc ttgccatcaa gcaggatctg gagggacccc cggctgttgg
    19921 gccattcccc tttgctaaca acggtaaaca ggtcgggtgt tgcatctccg gtgaattggc
    19981 ccacggcaag gctattgctg gattctgtac cggggatatg ccggttccat aagggttcca
    20041 tggttttccc atccacggca aaaatcctgc tgctgtggga aatggcgacc agatccagaa
    20101 atccatcctt gttgagatcg gccaggaccg gtggagcaat aaaaccatgg tgggaggtat
    20161 ccgtcgctac tgcacgtgca tggaccaggt ttttattctt aagatctgac aaaagcccaa
    20221 tgtataacga cccgctggct gtttctcccc ctgaaccaaa tacgatatac tgattcccgt
    20281 ccggttgctc gaaataaagg ggcgacatat aggattcctg tccatcgggc atggtatcgg
    20341 cggccagaat ggtgccggtg cggctgtcca taagcatcag gactcctgga aaacggtcgg
    20401 agaaactctg gggtttggct gcatggttgc ccccattctg aatgaggaga tcccggaggc
    20461 catctccgct ctgatcgggc acgaggacgc tgttgaaaaa attgaatcgg gcatatttga
    20521 gcaccggatc gttggtgtcc ggcggactgt aattccagaa cgtttcgccc gtcctgccgt
    20581 tcagggcacg cagataggcc catctgcctc cgatgatcac gtccagggtg ctgtcgccgt
    20641 tgacatcgac cagggtggct gatccgtaca cctggtccct ggagtccgta tgccaaatca
    20701 tctccccgga ctttccgtcc agggcgatga ccccgtaatc cgtggattga tattcgtttt
    20761 tacaggcccc cagcaccaga tcgtcggtcc cgtctccgtt gagatcggcg gcccggggtg
    20821 aggactgtga accgatgacc gggaagccct ggttccagac gaccgtggtt tctttattcc
    20881 ggcaggaggt caatccaagg gccacaaaaa aaaataccca caaaccctgt tcgtatcgat
    20941 tgccggacct catgctgtaa aaacttgaat cgtaaagata accagggatt gtttgaacga
    21001 ttctgtaatt ttccaatacc ctttcaactt ttctccccgg atacccggtc atccttgaat
    21061 ttatatttat tattgccgga aagtcggctt atgaattcaa aactaaaggt atttgtcagc
    21121 aggaaaattc cttctttggg tcctgacctc cttcggaagg aaggctttga agtgaagatc
    21181 tggccggagg aaagacccat gcaacaggac gagctgatcc aggaggccaa acaggccgac
    21241 attttactca gcctggggga gaataagatc gatccctatt ttctgaatga atgcaggcac
    21301 ctgaaaatga tctcccaatt cgcagtgggt tatgacaata tccatatccc cgaagccacc
    21361 cgcctgggca tcccggtagg aaacacgccc gatgtcctca gtgaagccac ggcagatatc
    21421 gcctttggcc tgatgatcgc ggtatcccgg aaattctttt ttaatcataa aatgatcctt
    21481 gccggtggct ggggctcctt taaaccaact tcccatctgg gtcaggagct ggccggcaag
    21541 accctgggaa tctttgggct gggctccatt ggcatggtca tggcccaacg ttgccaaggg
    21601 gcctttggaa tgaaagtcat ttattacaac cgcacttcca acgagctggc ggaaaaacaa
    21661 ttcaatgccc ggaaagtaag tttcgatgaa ttgttggccc aaagtgatgt cctgtccgtc
    21721 cacagtgtgc tgagcgatga aacccgcggc ttgttcgacc ggcaggtctt ttcaaaaatg
    21781 aaacccacgt ccatcttcat caataccgcc aggggaggaa tacaccatga agaagatctt
    21841 tacgaagccc tgcaaaataa ggtgatctgg ggagccggcc tggatgtaac caatccggag
    21901 cccatggaca aagacaaccc cttgttgcag atggaaaacg tggccatcct ccctcatatc
    21961 gggtccgcta cggtcgaagc ccgggatggc atgtcgaagc tggctgcgga aaatatcatt
    22021 tcatacatta aatcgggggt gatcccgcat tgcgtgaatc cggcggctat tgagtaagca
    22081 atagtcaata gtcaatagtc aagggtcaat ggtcaatagt cagtgagcaa gttcacagtt
    22141 cacgactcaa ccttgccttg gtcaataggt tttcgtcatg tccgcatcta cggccaggat
    22201 cacatcggcc ttccccaggt tgccgggatc cagtctttgg agtgaagttt gggagacggt
    22261 ggctatggta agatatttta attggggttt ttcctggagg agataccaaa ggatcccttc
    22321 ataattttcc gaatggtaac ttccatgata atggatgaaa agagtctccg gatttaggtt
    22381 ttgaagtata aaatgagcca tggtcgcgtc tttgatggcc tgggctttgg gtaggttggg
    22441 tccgccgtgg ccggccatta tagtcagcat gtttttataa ccggggagtt cggcatcgta
    22501 tttgacggga agcggggcga tccaggattt ttccaggtcc gacagggtat ccagggcttc
    22561 aaatccgttt tttgcaacca gggaggcaaa acgccgcggg atattggtcc cgataaatgg
    22621 gatcttgttt tgtttggcaa aattcaccag cggggcataa tccgttttat aattggacca
    22681 gagccgggcg gtggaatcca gttgttttgc cgtaatcgtt ccctgaagat aagcttcgag
    22741 ttcatcctgg ttgtcccgtt cgatcatttc cgctcccagc accaagggct gttgtttgct
    22801 gcaatcccgg gtcagcctga gttgaagcca gtgtgcgatc ggattattat gttcttctcc
    22861 aaaaaggaca aggtctttgc cggccgttct tttgagaagt tgggcatact tcagtttttt
    22921 ccctttcccc gtaaacaact ggtaagccgg cagggattgg gaaaacattc cggaaataac
    22981 aaccaggttc atgattacaa taatcgggag ctttaatttc atgggggaaa ggtataaaat
    23041 ggctgcgagt catgcgtcgc gaggtatgga caatccgcta aaatcaattt tattttaaaa
    23101 agaataattt aacctgaaac tggcattgaa tccggtttca tcggcataat accgcaggcg
    23161 gttcagataa tcccggtatg gggtgttgaa taaattgtcc accagcagtg aaaatttcaa
    23221 ccgggacttc cccaccgcgt aactgctgcc tccttccaat gaaaacaggg aatatccata
    23281 cggaggcagc aggaagtcct gtccgggttc cagccggttt tgcctgaaca cgaatttccc
    23341 gtgggcgccc aaataagtac ccgaccatct tttgcgatcg ccaaaagaat aagtcaggtc
    23401 tgattgaagg ctctgggcgg gcatgtaaac caggggaagt ccgcggctct ggtcctggcc
    23461 aatgacaagg gaagccctgg aagtccattt gacacttttg ttccattcgt aggaaaccag
    23521 caggtccgat ccatagagga gggcgtgggt ttgcatgtat tgatataccg gaaaagcccc
    23581 gcggatggtc aaccggaatt cgttgagcgg ttgcaaatag atataatccc aaatgggctg
    23641 caggtatccc agggcctgga taaaaaattt atggttcacc gcgccgtcaa gcccgacgat
    23701 ccatttaaag gacttttcct gtttaagccg gggattgcct tcttccagcc cgctcacccc
    23761 ctggtgcaat ccgaagctgt acaattcatt tacctctggg gacctaagga catatcccag
    23821 gttggtggtc aggctgagca cctcattcaa ttggtatttt ccgccaaagg aaacattgat
    23881 gttgttgaaa ttattattaa atcggacgat cctcctggga agctcccggg aaatggtagc
    23941 ggccgccaga tgcctgaggt catagcggac tcccatttcc cactgccctt tttcgaagct
    24001 gcgctgataa atcccgaaag ccgatccggt cagggcctgg taatccggga tcaagggcag
    24061 gacaccggta cccggctgat tggtgttata ggcatattga tattgaagac cgctcttgat
    24121 cagtacgccc ggacggggag tgacatgata gattccttcc acaaaatggc tgtattgcct
    24181 gaggctcagg gcaggcaggt cggagcgccc gcttcgccgg acgtcaaatt ccctgcgttg
    24241 attgatttgg cccccgtaat gaatggagag gatgtttttt tcgttgatca accatttgtt
    24301 ttccagtttg agcaggtggt gaaccactgc ttgccgcggg gacccgatcc cgtatgaaaa
    24361 ggcatcccgg gtataaaaag gcaccggccg cgtgatggcc tcttccaggt ccgacaggtt
    24421 tccgatatgc gatccccgga gtatcccgat ctccgtatca aacaggctgt agtacaaacg
    24481 gctggcccag cgggacccga attttttttc gaattggatg cccagattgt tttccttttt
    24541 cccggtattg gtcaaaaaat aatccggtgc ccgctggtcc ccctgccttt taaaagtgcc
    24601 gctcaacatc caggcggccc agggccggtt ttgttgcaaa cgggcgttga cggtatgccc
    24661 ccggccgttg ctttgaaaaa tataattcaa ctggccgtga agatggggat ctgcctcgat
    24721 ggggttactt tcagccagta ccactcctcc cagcccgttt ccaccgtagg cgagggagct
    24781 cacgcctttc actaccgtca gatgatcggt gagaaaggga tcgatttccg gggcatggtc
    24841 gttcccccat tgctgcccgg cctggaccag gccgttgttg atcatggtga tgcggttgcc
    24901 atgcagccca tggatcaccg gtttggaaat gccgcttccg ttccggatga cggagactcc
    24961 ggctattttt tccagcaggt ccgcgaggtt tttatttgat tccctggcga tctccgttct
    25021 ggcgatcgag ttggccattt gggtggaatt gtcttcccgg gaaccgtgaa ccgcgatttc
    25081 attgagcagt tctgcatggt gatgcaggtg aatgaataaa ctggtatccc ggtccacccg
    25141 caaatacagg ttttgattct cacacccgat gtggctgatc tgcaaatggt aaaatccggg
    25201 acaaatgccc tgaaacataa attgaccgag gctgtcggct gcaactccct gcctggtttc
    25261 ctggaggtaa acggccgcgt actccagggg tattccggtg ctctcatcga caatagtgcc
    25321 ttggatcacc aaccggcagc cttgactttg cccgattaac ggaatcagga ccaggagaag
    25381 aattgaagtg atttgccgga gcatatggac aaccggtttt cgacgaagat cattgtatct
    25441 ggacatcgaa tacaatttca aggtctgtct ctcctcccgc ctgcgaaagg tcccccttgg
    25501 agactccggg agcagttttg tcgggctcat gcctgagggt aatggtcaga ttacctgaag
    25561 aaacagaccg ggtgatcaat ttggtgataa gtcctaccgg atggccgttg gagtcggtat
    25621 cggcatactc gacgtttgcc aacagtccgc ctgaagtggc gagaaaaaac tggtgttcgg
    25681 tatcctcggt ttggatctcc tgtccgatgt cgaccagggg aaaggaaatt tcattgagga
    25741 tttccaggga gccggtgtat tccgtattgg ccgtgaggac acctcccgaa atcaggggat
    25801 cttctccgcc ttctccatca taatcctgaa aagtgaagac ggcgctattt cctccgttaa
    25861 ccggtgtgag ggtatatctc agggtggtga tcaattcttc ttcattgggg atctgggggt
    25921 catccagccg gcaatgagaa aaaatcaatg ctgaaatgag aagataagcc ggaagcagtt
    25981 taaacataaa atgaaattaa aatcagggta aatagaaaac ccgggaaacc ggaacacatc
    26041 acgcgcctga tataaagaca tcgtgcgaaa cctttcagat cgatgcctta aaccggcgct
    26101 acgatcaaca aattcctgga gggcccctga gcagggtggc agatacagtc cttctgtgcg
    26161 ctaaacccga atactccggc agcttatcct ggaattcctg ccgaacattt ttctggacct
    26221 tgcaggaagg cgctataaaa acctgggcgg tgaatgcatc gcacagatca caatgatgct
    26281 ggacgctgtg caggtggagg ctggaagtgt cgtggcagtg gttttcctgc aaatgaaacc
    26341 attcatgcgc cgtgtttaaa acgggaggca gggccagtaa aaaggccagg gatatggatg
    26401 atatgaacct gaagaagcgc acctgttgtt gcaaagataa gggggaaaag ggattgaggt
    26461 cccggggata agagaggatt gaggagtgag gagagaggat tgaggattga ggattgaggg
    26521 gggcctcctc atcatccctg atcataagcc tttttgagcc agtctataag ctcctgattg
    26581 atcatttttt catcctccac cttgatccgg tgggtgcaca tgctgttcca gcttcccgcc
    26641 gccgtcgcca ggccggccgg ggctatccct ttaatattaa gtcctacgtc gagccggtcc
    26701 ttggtagagg gctggatcag ggcaaactgt tttttccgac gcaggctcac ataggttttt
    26761 ttaggggcgg tttcgatatc cgttccaaat tggccgatgg cggtcatgat tttatcgtac
    26821 cagggtttga ggttctcctt gcctttgtat tgttcggaaa tccagtcggt atcgttccct
    26881 tcggcgccag catggctttg tttggcaaca tggaccagca cattggcatt gccatgggtg
    26941 agcccgtgct tttctttgag caggctcacc agttcaccat gcttgctcag gccgctggaa
    27001 aggatgatcc gggtccactc ctccaggttc ttgccggtgg atttttcaat attggcaagt
    27061 tgggtagccg tagcgttcgc gaccgggtcg ccgccggcag ccttgtctat tgatttggcc
    27121 ataaaatatt tttctttaaa aatagaaaga gttgggaaaa agattcaaat acgacacgga
    27181 tctgcctttg aaatatcctt aatgaaaggt agctgtttta cctgcttccg gcgtttaact
    27241 ccggattatt gactaatttt atttttcaag caaaaaaatc ctatatgttc agacgagaat
    27301 ttctcaaaca cacgtcttta ggaaccctgg cactgggtgc atttccttac ggattgacgg
    27361 aagacgcaaa aaagacctcc cgccttggcc ttcaattgta ttcggtgagg gatgaaatga
    27421 aaaacaatcc ggtagcgacc gtccaggcgc ttggcaaaat gggttaccgt gactgcgagc
    27481 acgccggtta caacgggcaa acccgaaaat attacgggta ttcccccgcc gaatggaaaa
    27541 aaattctggg cgatgcgggc ctgaccatga aaagcggcca taccaccttt gcctccatgc
    27601 actacaatat ggagagcaag cagctcaccg atgcctggaa gaccgtcgtg gaagatgcag
    27661 tactcgcggg ccaggaatat gtcatcagtc catggctgga catcagcctg agaaaaaact
    27721 ttgacgacct caaacgtttt atggaaggtt tcaatgccgc cggagcctat tgcaaaagcc
    27781 agggcatccg gtacggctac cacaaccatg attttgaatt cagcatcaaa ctcaccagcc
    27841 agcggatcat ggatatcatc atggaaaaca ccgaccccgg cctggtcgct cagcagctgg
    27901 acatcggtaa catgtacgga ggcggcggaa gggccatgga attgctcaaa caatatcccg
    27961 gccggtttga actcatgcac gtcaaggatg tgatcaaagc aagtaccgac ggcctggcac
    28021 attacgaaag taccgttctc ggcaaaggct tgatcgacgt aaagaaggtc ctcaaatatg
    28081 ccctccgtaa agggggtacc aaatacctga tcatcgaaca ggaagcttat cagggcatgg
    28141 cgcccctgga ctccatgaaa gaagatctcc ggatcatgaa ggattggggg tatgcaggtt
    28201 agagggttga ggggtgagga ttgaggggtg agggttgagg attgatgctc aattgaaaaa
    28261 attataaata tccggcagga tgacggtgtc cccgccgtcg ttgacgattt tcaaatcgta
    28321 ccgcaccact atccttgttc cgacaggggc gtggtagtat aataaccatg cttcaccctc
    28381 agaaaaggcg atgcatccat tggaataggc ttttcccagg gttttgggat tggtggtggg
    28441 gtgtagcagt tggccgggcc tttgaccgtt tatttcgggt tcgagccagg ggatcagggg
    28501 catcagggtg gatttgccat cgtcgcgggt ggtatgggtc tgtttaatgc ctgtgtgcgg
    28561 gtcaaaaaat acggttggct cgcggttgat gcgtacgatc ctgccctctc cgggaacggt
    28621 cctgaggtct tcaaaccgcc cggggaccgc ctgatatcgt tgggtatttt gcccaacgcg
    28681 gatattcatg caatgaatga cttgatcttt ttcatacagc cgaaggctga attcggggat
    28741 attgatatct atccaatttt ggttgagccg gttctggatg agggccgcgg ttgcggaatc
    28801 cgggataaag aggctgtctc cctcatggag caccgtcagt tgcctttgat cgtaaacaaa
    28861 aatccccctt tgcttttgat gataataatc ggtggtttga aggctgtcga tgatccaggg
    28921 attggcatgc accaggaggt gttcagacag ggcatagggg accctggacc ggtattccga
    28981 tacgatgctg tccagggtgc ggaaataatt cttgacggga atgtcccgcg atacccggat
    29041 accatacagg ggaccggatt tgacaagctt cgcgcctgag gtctcgatga tttttgtttc
    29101 cgaaccgggt tccctttcca gtggggtccg acaggaaacc ataaacatcc cggtcaaaaa
    29161 aagataaatg aacggtcctg ccttccccat gttcgaatgt ttcacttcct cataacggta
    29221 tttgtccgcc tattgttctg tggaacgaaa caaccggatg cataactgct aagcgtaaag
    29281 cagctcatgt cattcggagg gaagattctt ccgtgaattc acccccgtgg aattcaattc
    29341 ggcttcatcc actatcttgc agccatgaaa ccggtccttt tttttctttt tgtttttttg
    29401 gcatgttccg cagttgctca aaaaattcct tctccttcgg aattcctgcc ctccccctat
    29461 accaaaatgt ttacccacca tgcccatctg gtggattatt tcaaaatggt agcggaaaaa
    29521 agcaaacaag tcaaactggt ccagtatggg gaaacctacg aacaccgtcc cctgctcctg
    29581 gccttcgtat cctccgaagc caatatccag cgcctcgaag acctccgggt caacaacctc
    29641 aggcgtaccg ggcttttacc gggcaatccc gataaaagtg ataaaacggt gtttgtctgg
    29701 ttttcttatt ccgtgcacgg caatgaaagc gccggatccg aaagcagcat gcaggtcctc
    29761 tacgacctgg tcaatccgga ccatggagat gcccgggaat ggttgaaaaa taccgtggtg
    29821 gtcatcgatc cttccgtaaa tccggatgga tacagccgtt atacccaatg gtattacaac
    29881 gcggccaatg aaagagtcag ccccatgcgg gcaaaccggg aacaccggga gccctggccc
    29941 ggaggccggt ccaatcatta tttgtttgac ctcaaccgcg actgggcctg gcaaacccag
    30001 gcggaaagcc gtcagcgcct gaaaatttac aatgaatgga tgccgcaggt ccacaccgat
    30061 atccacgaaa tgggcaacaa ttcgccatat tatttcgctc cggcggccaa accctatcac
    30121 caatacatca ccccatttca ggcttcattc cagaccgaaa tcggcaaaaa caatgcccgg
    30181 cattttgatc aaaacggctg gctctatttt acccgtgagg tattcgacct gttttatccc
    30241 agttacgggg atacttatcc cacctattcc ggagcgatcg gcatgaccta tgagcagggc
    30301 ggtatcggag cgggcagggc catactgacc gaaaacggcg ataccctcac cctgtccgac
    30361 cgcatccagc atcaccgtac agcctccctc tccacgattg aggtgagcag taaaaatgcg
    30421 gcaaagctcc tggatcagtt ccaggaatat tttgaccgga acaaccacaa cccacagggc
    30481 cgttataaaa cctatatcgt caaagggtcc aacgatcctg gaaaacttcg gtctttctgt
    30541 tccctcctgg ataaaaacca gatccgttac gggaggacgg accgccagag aaaagtaacc
    30601 gcttataatt atgagaatgg aaaggaagag ccggtttcgg tggaatcttc ggaccttctg
    30661 atcagtaccc atcagcccaa atccgttctg ctccaggtcc tccttgaccc tgaaagtcac
    30721 ctggaggatt ccgccactta tgacatcacc gcctggtccc tgcctcatgc ctatgccctg
    30781 gaaacctatg ccaccgccga aaggattaat ccggacaagc cgtttgaacc caatggcagt
    30841 aaagtcgtgt atgcgccgtc cgcttatgcc tatatagcca cctggaattc atttgaggat
    30901 gcccggttcc tggctgcgct ctccgatctg ggtattcgtt caaggtttgc gacgcgcgat
    30961 tttaaattgg aaggcaaaaa ctttcagcga ggcagcctga tcgtcacagc ggcggacaac
    31021 cgcaagacgg aagcttttca caaaatcatc caggacgcgg cgaggacaca tcataaaagc
    31081 ctcacccagg tcagtaccgg gttttcagac cagggctatg acctgggatc gtcctatatg
    31141 gaactggccc ggatccccaa agtgctggtc ctcggtgggg aggaaagttc atccctgtcc
    31201 tatggagcga cctggtattt ttttgaagaa aatctgcaat atccggttga tcttgtggac
    31261 agccggcaac tgggttcggt agaccttcgt gaattcaata ccattatcct gcctgacggc
    31321 cggtttacca tttcggaaaa gctcaagtcc tgggtgtcgg aagggggcaa cctcattgcc
    31381 ttgggctcgg ccctgaacgc gtttgcggat aaagaagggt tcgccctgaa atcctatgaa
    31441 tcggaagacg ccaaaaagga agctgaaaag gaaaaagaaa aacaagacct ggatcggcga
    31501 aaatttccat tggaccagga agaacgagac ggcctgatgg attttattcc cggggctatt
    31561 ctcaagacca gcctggatac ctcccatccc atggcatttg gtttaggccg ttcctatttc
    31621 accctgaaga cggaggactt aaaattcccc ctcatcaaag gaggaaaaaa cgtgatctac
    31681 ctggaaaagg actggaaaca atcgggattc atcggcagcc gcctgaggga taagctcaag
    31741 gaaaccaccg tagtcagtgt ggaggcaatg ggcaggggcc ggattgttta tatggtggac
    31801 aatcc
//
LOCUS       DAOQXT010000049        31719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1674_length_31719_cov_5.351030, whole genome shotgun sequence.
ACCESSION   DAOQXT010000049 DAOQXT010000000
VERSION     DAOQXT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 31719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31719
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1674_length_31719_cov_5.351030"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKM27_10105"
     CDS             <1..730
                     /locus_tag="PKM27_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNR07655.1"
                     /translation="FTLIIAVPSAVKVFNWLTTLYGGNIRLTTAALYGIGFVSMFISG
                     GLTGIFLGNSAIDVQQHDTYFVVAHFHIVMGVAAMFGMFAGVYHWYPKMYGRFMNETL
                     GKIHFWGTMIGAYAIFWPMHYIGMAGVPRRYYRFDTFEAFKHFGALNKFMTIAAIIVF
                     ACQILFIINFFYSIWKGRKVTSRNPYNANTLEWTTETIVPGHGNWEGNIPTVQRWAYD
                     YNKDEREFIPQYVPVGPDEKDESH"
     gene            770..1675
                     /gene="cyoE"
                     /locus_tag="PKM27_10110"
     CDS             770..1675
                     /gene="cyoE"
                     /locus_tag="PKM27_10110"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNR07656.1"
                     /translation="MKTGETYTISYAMDGLKAKVGDLGQLVKHKLTFFVVLSSVVGYL
                     LAATGEIQWFQVVLLALGGFMITGSANALNEIIERDYDALMTRTAGRPLPSGRMEVTE
                     AVIWAGVMGVAGILLLAFFNLITAVLGAASLILYAFVYTPLKRITPLSVAVGAIPGAL
                     PAMIGCIALEGKITLLSITLFGIQFFWQFPHFWSIGWLGFDEYRKAGYKIMPLPSRER
                     DHSIGMYSMIYALFLIPISLMFLFYPGVSNYAVAGLCLAAFGYAVAAWGLFIHNDLAR
                     ARKLLFTSLFYLPIIQIILLTDKWL"
     gene            1666..2244
                     /locus_tag="PKM27_10115"
     CDS             1666..2244
                     /locus_tag="PKM27_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNR07657.1"
                     /translation="MALTQSGPVNRFGIHPQKFALWAAMGSIVMMFASLTSAYVVRRG
                     AGNWLEYKLPLLFFISTLVLLISSLTLHWSYSSYKKGLELRYKSFLVVTLLLGLAFAV
                     LQYQGWLQLYSYGIELKGNPSGSFFYAISGIHLAHILGGLAALTVAGIHAFGLPFAVS
                     EKRRHRFELVCHYWHFVDVLWVYLLTFMIIQQ"
     gene            2264..3139
                     /locus_tag="PKM27_10120"
     CDS             2264..3139
                     /locus_tag="PKM27_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNR07658.1"
                     /translation="MTTDTIKHGAHEDQAHQEGGNLWGGGREPFKASYGKLMMWYFLL
                     SDAFTFSGFLISYGALRFSMPTWPLPDHVFETIPFYSGKAPLIFVTFMTFVLIISSVT
                     MVRAVQEGHRENKKGVVFWMLLTILGGITFLSCQAWEWTTLLAKEHVSIHRNPFSRHM
                     EAGVYLNEDGTPIVKDGAEETFKKGDSYLIHKHDGEWDLRKYRVTEGPDAGQVKQLAF
                     GPKAFGALFFFITGFHGFHVFSGVLFLAIIAINAASGVYVARKNGYEMVEKIGLYWHF
                     VDLVWVFVFLVFYLL"
     gene            3186..3611
                     /locus_tag="PKM27_10125"
     CDS             3186..3611
                     /locus_tag="PKM27_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNR07659.1"
                     /translation="MSAHLSYEESKKVVFKGLILLGVITLIEVFIALFGNGHVISGFH
                     LPKIIMYPMMIGLSLYKAYFIVYEFMHMRYEVRGLALAVLLPMVLLIWAMIAFFNEGN
                     AWFNYRDYVRTKNKTEVKTSIQPQGDKKPAETLEIQEIH"
     gene            3608..4135
                     /locus_tag="PKM27_10130"
     CDS             3608..4135
                     /locus_tag="PKM27_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07660.1"
                     /translation="MSPKPEGGVKKNRLALWISLFILLALPAISLYYSFQGSLLRKKS
                     LVELSPKGRLDPGLASLLSGEFNYRVIGSACEDVERTGALIDQFAQEKIQFVILQDSA
                     FEQRFPARQVEHWKKQNILLILPDPGPHLPGDQNACTFVLVNKNSEILNHYNFTEPVD
                     RRKLVEHLAFLVTSK"
     gene            4147..6078
                     /locus_tag="PKM27_10135"
     CDS             4147..6078
                     /locus_tag="PKM27_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR07661.1"
                     /translation="MRLRAAKNSLYTLALRLGLVYAAYMLSRVIFYLLNYKTFGFIPL
                     AEWPEIIMGGLLFDTSAIAYTNILFILLALFPVKEIISLRSYQRLYDAAFLTVNGACL
                     IINAVDFFYFRYNLKRITADFAHWAGESNVMRIMMSFAGENWYVFVLAAGLLYLFYKW
                     YRRIPLYVTLLPNAWLFGFVHLLLLSGMTGLSIAGMRGGFLHSTRPITLSNAGKYVSR
                     PDLMPVVLNTPFSLIRTFDKKAYPEKTYMPEEEAIQVFNPVKNYSDSLPFEAKNVVIL
                     IIESLSREHSGYLNPNLGGGEYKGYTPFLDSLMKESLVCTNAFANGRKSIDAMPSILA
                     GIPSLVLPYVLSHNSLNKINSLASLLKPKGYRSAFFHGAPNSSMGFQAFSRIAGFDQY
                     YGKDEYGNDADYDGIWGIWDEEFLQYFGRQLDTFTPPFLGICFTLSSHHPFKVPPRYE
                     GVFPEGKLPVHACVSYTDMALRKFFEVASTKPWYRNTLFVITADHSTVAWSEKYNSPV
                     GAFAIPILYFSPGGLLRKTYQGVTQQTDIMPSVLDYLHFDQPFVAFGNSIFDDEAEPF
                     ALHYNNEQYQVIDSDHRLSLFDGNKITGVYDIRDSWSLGGNKVLLPAESESELLRFHQ
                     AVIQQYNQRILHNKLTVHQ"
     gene            6088..6633
                     /locus_tag="PKM27_10140"
     CDS             6088..6633
                     /locus_tag="PKM27_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNR07662.1"
                     /translation="MPKSPVPEYNPALAKKLNGIAIVFSVVVVLIVASLRRFHVDTPV
                     DFRFLAPLHSSLNALTALGLLLAYYFIKQKKILWHKRVMILNMVFSALFLVSYIIYHL
                     TTPETRYCHEGVIRYVYFFILITHVILASFILPVVLFTFIRAITGQVEKHRNLARWTF
                     PVWLYIAVSGPVAYLMLRTCL"
     gene            6669..6923
                     /locus_tag="PKM27_10145"
     CDS             6669..6923
                     /locus_tag="PKM27_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07663.1"
                     /translation="MKRTGIVVMIVLLLVTESLMAQCPMCRMSLESNLEDGGTMGRTI
                     NAGILYMLGMPYLLVGLLGYIWYKNRKKMEAGQEEQTNLN"
     gene            7093..7875
                     /locus_tag="PKM27_10150"
     CDS             7093..7875
                     /locus_tag="PKM27_10150"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR07664.1"
                     /translation="MILKNKNILVIGGSGGLGLAACLVFLQEGANVFASGLGPPEALP
                     VSSSNFTYLVSDAANEDAVDRLVKEALTRLPQIHGLYHIAGGSGRKAGDGPLHLMSLE
                     GWDYTLNLNLKSIMLSNRAIIRYWMDNHLPGVILNMGSVLASSPSAQYFSTHAYAAAK
                     AGVEGFSRSLAAYYARSNIRVNVIAPGLIETGMSTRAQEDPAIRAFIKTKQPLDGGRM
                     GKPADCAGTAAFLLSDLSSFISGEVIHVDGGWQLSDGQYYTK"
     gene            7898..8785
                     /locus_tag="PKM27_10155"
     CDS             7898..8785
                     /locus_tag="PKM27_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR07665.1"
                     /translation="MIAGIDIGATNIKAVVLDQGYALVDKFQGPTKDDGKTKSWMAEV
                     KNMVDTVVKKYPDLSCMGLSAPGIPDEGNTCISFMPGRLYGLEGLVWSQYLGIPSCVL
                     NDANASMVAESRIGAAKGYEHAIMLTLGTGVGGSILIHGKLYTGFRQVGGHLGHLSID
                     MHNSFRSITGMPGSLEDAIGDSTVKARTGGRYASTTELVNAVREGEEEAQKVWLESVR
                     ALAIAVAGLANVLSPQALVLGGGIASAGNLLMEPFLRFLDEYEWRPGGKKMEIKTARL
                     GPFAGATGAGIFASEYFKS"
     gene            8782..9573
                     /locus_tag="PKM27_10160"
     CDS             8782..9573
                     /locus_tag="PKM27_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HNR07666.1"
                     /translation="MSLTDQYLDKCLALAERVRAQHSKILEAAEWFAGSILKGRVVHL
                     FGSGHSRIMVEEMWPRYGSFPGFNPIVELSMTFHNLVTGANGQRQAMYIENMPGLAEK
                     ILRNFNLSELDTALVISSSGTNIVPCEMAEIFRENKIKVVSIVTLDHLQKSESKRSDR
                     KKLTDFSDLVLDNGAPAGDAMVRIDGMEAPVSPGSTVGSVLLINSIKAELANLLMLGG
                     QPPNALVAACLAGAEKASHIFNEAYDEHATRMSRLYNFYKPIEIR"
     gene            9570..10610
                     /locus_tag="PKM27_10165"
     CDS             9570..10610
                     /locus_tag="PKM27_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HNR07667.1"
                     /translation="MTRTNTLRTTVVGSYPFPAWLEFASRNLHQFGSADIEEMIEDAV
                     QVAIQDQLAADLDVITDGEQTRIDFNLSFYGYLDGIEQESEAPRKWGPAAHDQRGKHK
                     ITGAVRAKHGLGTVAEFKRLQKLAGPSGKVLKCSVPGPYTLSGRLDPNGIYADRWEVT
                     EALVPIVADELRKLVEAGCTEITVDEPSMSCYAYKEDPLRFVDIFNRTVQAVRGKCFL
                     STHLCFGNYKGRPVGKRTLAPMLPAFLDLAIDEVHIEMANREFAEIERLSDFAGRMRV
                     AVGIIDVKNYYIESVEEVVFRIKQCLKFVPADLLSVAPDCGLSQTARWASRQKLKNMV
                     LGARAVREEKGL"
     gene            10620..11462
                     /locus_tag="PKM27_10170"
     CDS             10620..11462
                     /locus_tag="PKM27_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR07668.1"
                     /translation="MIKAVQKDDAFLTDLRGVGEGRARLDIWWLGQSGFLLHWNGKNI
                     LLDPYLSDSLTLKYQGTAKPHIRMCERVVDPAKLQFMHYVTSSHVHTDHLDPDTLAPL
                     FANNPGLKLILPLAHLREGMKRSGCSGERMGGLDAGQTFAEDEISFTAVPAAHETLQV
                     DEEGHHLFLGYIVRAGPWTLYHSGDTVLYPGMAELLKPYAPDVSFLPINGTDPSRGVS
                     GNMNAREAVWLAKNIDSKLTIPCHFHLFEFNTVEPDEFIETAQKEQVPIRVMQVGERL
                     SLQL"
     gene            11500..12801
                     /locus_tag="PKM27_10175"
     CDS             11500..12801
                     /locus_tag="PKM27_10175"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR07669.1"
                     /translation="MNIPESPLPRIVIVGAGFAGLKLARRLVGSKYQVVLIDKNNYHQ
                     FQPLFYQVAMAGLEPSTISFPLRRILRSDAKVFIRIAEVHSVNPERNELNTDIGSLKY
                     DYLILATGAKTSFFGNKNIEDMVLTLKSLSESLLVRNTILDHYETALTESDHEKKQGY
                     VDIVIIGGGPSGVELAGSLAEMKRYILPKDYNELNPKEVDIYLIEANNHLLPSMSKKS
                     SQRAERYLQKMGVLVMKNNRVTNVDADFVYLQDGSRIMSKNVIWTAGLEGAVIPGIPR
                     EDLVKANRIRVDRYQQTPSYKNVFVVGDLAYMEEPDYPNGHPQVAQVAIQQAKNLAYN
                     FKRLHQPGYVWRPFSYRDLGTLATIGRNKAVAEFKWVTVHGFIAWVLWLFVHLFQLLG
                     VRNKLFVFINWVANYLSYDQALRFIIKPKKDKRSELKKSKA"
     gene            12871..13482
                     /locus_tag="PKM27_10180"
     CDS             12871..13482
                     /locus_tag="PKM27_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07670.1"
                     /translation="MAWNWLSDRNPGYQKIAQRLGLDYEEKDQDKEILRAHKGYHLFK
                     TGSNKKVKYILSGLLESKEPFRVFEYSFAVSTGKSTTVFTQHVLSVRLKKSLPPFTLR
                     PENLFHKIGEWLGWKDIDFDAFPDFSKQYRLMSGKESSVRKVFNDHILGFLSFEKGWW
                     VECDGPTIIYFKHSKRMNEEMVEPFIQVSQTMHYFLTGFKGIR"
     gene            complement(13559..14257)
                     /locus_tag="PKM27_10185"
     CDS             complement(13559..14257)
                     /locus_tag="PKM27_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR07671.1"
                     /translation="MENLIGKVIYITGGTKGIGLGIAVKALEAGLKVAVSARHIDHFD
                     DKPFPPNQFLLIRSDVKNKEDEIQAVEQIIKKWGKLDVLVANAGVGHFAPFDQLSDEQ
                     WHDTLDTNLTGVFHSVQASLPALKKSNGYIITIASLAGTNFFVNGTAYNASKFGLVGF
                     TQALMLDLRPHQIKVTTIMPGSVATFFNNHIPNEKDFWKIQPEDIGQMVIDLLKMHPR
                     TLPSKIEVRPSIPA"
     gene            complement(14361..14954)
                     /locus_tag="PKM27_10190"
     CDS             complement(14361..14954)
                     /locus_tag="PKM27_10190"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNR07672.1"
                     /translation="MKLFYSTLLAVFTAAVALAGNPVKPVAYKVDPVNSKIEWTAYKM
                     TGQHTGTLQVKQGALEFNGNKLIGGRFDIDMQSMTVTDIQGSMMERLLSHLKNDDFFS
                     VDKFGTSSLVIKKAEQKSANLYKITADLIIKGISKEITFDATVNQSEGNASASLKVDR
                     TLFDIKYRSGRFFPEIGDKMIKDEFDLKVNLTFAKSI"
     gene            complement(15052..16839)
                     /gene="sppA"
                     /locus_tag="PKM27_10195"
     CDS             complement(15052..16839)
                     /gene="sppA"
                     /locus_tag="PKM27_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNR07673.1"
                     /translation="MNFLKILLGSCLGTLLAVFLVFFIGLGILSVVASSAGEPGKKAL
                     DKPGFLEIRADLYYPEKTNNVRSTGVFDLNEQHIGLHDLVQSIQHAASDPKIKGILYR
                     SGYSSLGSASALLIRQALLEFKASGKPIYAYGEYFTEGSYSMASVADRILLNPNGVVE
                     LKGFGAMIPFFKEFLDKTGISFEVYYAGQFKSATEPVRFNKMSDQNRSQIREYLEDLY
                     RLHLEGISESRGIAVSDLRNISNEYLSRSASDAIRFKLADTLAYQDDVYRMIQNDLGL
                     SETARPSIFSVNRYFESFDHQPAHAPAATAGRIAIVFAEGEILDGEGHNGQVGSARYL
                     RILRKIRQDDKIKALVLRINSPGGSSMASDNILHEINLIREAGKPVVVSMGDYAASGG
                     YYIACSADSIFAEPNTITGSIGVFFMIPNLTKLMGDKLGIDMDTVRTGKFTTAFTPFM
                     PWSSEESLIAQQQTDMVYDRFLSVVSKGRNMDKDDVEAVAQGRVWSGVKALEHGLVDR
                     LGGMQDALDCAVRMAKLDEYKVLEYPAMKDPIQQLLEDLTSKEPEITESIIQNKLTAW
                     FRPYARFASWTKEQAGKPLMRMPFELSIR"
     gene            17103..18053
                     /locus_tag="PKM27_10200"
     CDS             17103..18053
                     /locus_tag="PKM27_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNR07674.1"
                     /translation="MIQFYAREDNHLRQLQQAAPRCWINLHPPFEQNELESLAASKDI
                     PLDYLTDSLDPDERSRYERWDDNLRFILIQTPILNPDEEENQPIYITVPIGLLISPEF
                     ILTISAHENTILKKFIDGKVKNFDPSDTRSCILQILDQNVWAYLDSLKRLNLKRNLIE
                     QELYDSSRNAELKQLLRIEKSLVYFVNSLSANDLVKLKMKRTDFLQIAQDEEKADLFE
                     DILIDNNQALDMANRHTNILSGTMEAYASIVSNNLNKFIQRLTVVTIILMVPTLVASF
                     FGMNVHLPLSDKPMAFFYILFVSGLIIVFLVWFFRRKQMF"
     gene            18482..19459
                     /locus_tag="PKM27_10205"
     CDS             18482..19459
                     /locus_tag="PKM27_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNR07675.1"
                     /translation="MEQRVEPLLQENPNRFVVFPLQHNDIWQFYKDSVAGFWVTEEID
                     LAQDIITWQEKLTEDERHFIKHVLAFFAASDGIVNENLAENMLRMVQYPEAKFFYGYQ
                     IMIENIHSETYSLLIDTYIKDPSEKDYLFHAIDHIPCVRKKADWALRWISNGTFQQQL
                     IAFAAVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISRDEGQHCDFACLLYNKHIVN
                     KLPKAEVEEIIRDAVTIEKEFVTDALPVKLIGMNADLMCQYIEFVADRLLLALGNEKI
                     YKVDNPFPFMEMISLSGKTNFFEKRVGEYKKSGIGTTRENQVFSVDTEF"
     gene            19635..22118
                     /locus_tag="PKM27_10210"
     CDS             19635..22118
                     /locus_tag="PKM27_10210"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNR07676.1"
                     /translation="MKVLKRSGKQEEVSFDKITARIKKLCYGLNSTYVEPIEIAKRVI
                     IGLYDGVKTTDLDVLAAETAASLATVHPDYALLAARIAVSNLHKETKKSFSETIHDLY
                     TYIDPKNGQPAGLIGKETHDIVMRHADRLDSAIIYDRDYSFDYFGFKTLERSYLLRMD
                     GQVVERPQHMLMRAAVGIHGENLEAAIETYELMSEKWFIHATPTLFNAGTPKPQLSSC
                     FLLSMTDDSISGIFETLARCAKISQSAGGVGLSIHNIRAQGSYIKGTGGTSNGIIPML
                     RVYNDTARYVDQGGGKRKGAFAIYLEPWHADVFDFLELKKNNGKEEVRARDLFYALWI
                     PDLFMERVKADGNWSLFCPNEAPGLYDAYGADFEKLYTQYEAQGKARKVIRAQDLWFA
                     ILESQVETGTPYILYKDAANQKSNQKNLGTIRSSNLCTEIMEYTSPDEVAVCNLASLS
                     LPKYVKANKEFDHKKLMEITRVITRNLNRIIDINYYPIEEARNSNLRHRPIGIGVQGL
                     ADTFILMGYAFDSPEARQLNREIFETIYYGALLESCTLAEKDGAYSSFKGSPASQGIL
                     QYDMWEVTPTDRWDFPALKKQIKKHGLRNSLLLAPMPTASTSQILGNNECFEPYTSNI
                     YTRRTLSGEYVVVNKHLLNDLVNLGLWTKEIREKIIASNGSIQHIMEIPATIRDLYKT
                     AWELSQKVLIDMSADRGAYICQSQSLNLFVENPSFAKLSSMHFYGWSKGLKTGMYYLR
                     SKAAVDPIKFTLSEDYQKKFVTKEPDPRMTVSDLDEQDQPAGRAARPQGVPSVAPRET
                     VAALVDEPVIAAGIKACSLDDPGCEACSA"
     gene            22456..22947
                     /locus_tag="PKM27_10215"
     CDS             22456..22947
                     /locus_tag="PKM27_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNR07677.1"
                     /translation="MIAQAEAGILTRLEKILHDFIQELTPAKHKKPLKIKEILDDKRL
                     VMLMIRHGLPYSFYTLIESLAPFNESDWAAYFGISTKSLQRYKKAGERFKPLQSEKIM
                     EMAEVIYLGLELFGSADKFNAWLNQSNLALGGEKPVDLLKDSYGKSLVTGELTRISHG
                     IFV"
     gene            22986..23441
                     /locus_tag="PKM27_10220"
     CDS             22986..23441
                     /locus_tag="PKM27_10220"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNR07678.1"
                     /translation="MEVYRLCRKIYSGSLSGKGAALEGARWNSPGIEMIYTAMNRSLA
                     MVEVAVHLNLSAMPDDYYILTIHIPDELSLKKLNLSSLPDHWNDFPYHRETQAIGDAF
                     IKSGRYCLLQVPSAVTRGDFNLLINPVHPEFRRIRIVEREHFPFDKRFI"
     gene            23746..24063
                     /locus_tag="PKM27_10225"
     CDS             23746..24063
                     /locus_tag="PKM27_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNR07679.1"
                     /translation="MNIYVSNLGYQANDEDLKSLFSAFGPVVSAKVIMDKITGRSRGF
                     GFVEMTNEADGKTAIQNLNNQNVEGRSISVSVARERDASGNSNINRSFGSNSNSGGYK
                     KRW"
     gene            complement(24162..27749)
                     /gene="nifJ"
                     /locus_tag="PKM27_10230"
     CDS             complement(24162..27749)
                     /gene="nifJ"
                     /locus_tag="PKM27_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNR07680.1"
                     /translation="MKAKERMQATMDGNEAVAHVAYRINEVCAIYPITPSSTMAELAD
                     EWSAKKIKNIWGQVPDVIEMQSEGGAAGTLHGSLQTGALTTTFTASQGLLLMLPNMYK
                     IAGELTAAVFHVAARSLAAQGLSIFGDHGDVMAARMTGFGMISSANVQEAHDFALISQ
                     AVSLESRIPFIHFFDGFRTSHEVNKLEILSEEDLRTMINEDLVIRHRQRGLNPEKPFI
                     RGTAQNPDVYFQGRETVNRFYSDTPDIMEKTMARFDELTGRSYHLFQYTGHPEAERII
                     VVMGSAGDTIRETVLHLNAAGEKLGVIQVRLYRPFSMEHLLNALPASCRSLAVLDRTK
                     EPGASGEPLYNDVVVALNEAYASEKIGRLPKTIGGRYGLSSKEFTPAMVKAVFDELTK
                     SNPKNHFTIGIRDDVSHTSLDYDETFTLDESSWSQALFFGLGADGTVGANKNTIKIIG
                     ENTDLFAQGYFVYDSKKSGAKTVSHLRFGPKPIFAPYLIRQADFIACHQFNFLEKDDL
                     LKYARQGASLLLNAPFPTEQVWDRLPRLVQVKAREKEMKIFVIDANRVALETGMAGRI
                     NTIMQTCFFALSGVLPREEAIQHIKKAIEKTYSKKGAKTIQKNFEAVDQSLSNLFELK
                     IPARDTGTKTAEEVFSLTDEAPEFVRQVTARMMMGLGDELPVSLMPVDGTFPSGTTKW
                     EKRNIAESIPLWRDDLCIQCGNCSFVCPHSVIRSKFYHRGFLEDQPEHFQSAPINAKG
                     FPDTRFTLQIYSEDCTGCSLCVQVCPAHDPKDQSVKAINMKPRRERLDMDRDRENIAF
                     FEALPENDRTKFNFSTVHGVQFLEPLFEFSGACAGCGETPYIKLITQLFGDRLVVANA
                     TGCSSIYGGNLPTTPWTTNKEGKGPAWSNSLFEDNAEFGLGMRISLDKQGEMARDLLW
                     KMKDVVGVERVQSILDNKQSNDTEIQTQRRLVAELKKILQGMNSREAIQLGSVADHLI
                     KHSVWLIGGDGWAYDIGSGGLDHVLSTGKDVNIIVLDTEVYSNTGGQMSKATPTAASA
                     KFAAAGKRVGKKDLAMQAISYGNVYVAQIAMGANPQQTLLALREAEAYPGPSLILAYS
                     HCIAHGIDMEKGLNQQKNAVQSGYWPLIRYNPMLRKADQNPFILDSLRPSMSLKDYAY
                     QELRYKVLTKSNPVEAEHLMQLAQEIVDLRWRNYEEMAGFSAKDFQPVH"
     gene            complement(27763..29385)
                     /locus_tag="PKM27_10235"
     CDS             complement(27763..29385)
                     /locus_tag="PKM27_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNR07681.1"
                     /translation="MKNKELTPRADLSKDIMGIGPKRTRKPVYQDYLPPCNHACPAGE
                     NIQAWLGLVQEARYYEAWLKLTEDNPFPAIHGRVCYHPCEDHCNRGQTDTSVSIHSIE
                     RFLGDRAIEENWQFMAPESTSGHKVLIIGAGPSGLSAACHLARLGHEVTVYEAGPMAG
                     GMMHFGIPAYRLPRNIIHAEIKRLEALGVRFKLNYKVSDIVAEKKAGGFDAVFVAIGA
                     HIGKKVKIPAREAGKILDAVTFLKDVETGNAPKLGRKVVVYGGGNTAMDAARTVKRLG
                     ADEAMIIYRRDREHMPAHAFEADEALSEGVQIHWLRSIKEITETSFTVEVMRIEEGKP
                     VGTGVYETIEADALILALGQETDTAFLKKVPGIDINDEGVVVVGPNMMTGAYGIFAGG
                     DMVPSERTVTVAVGHGKKAARFIHAFLRGEEYHKPPAHPLISYESLHLWYDTEAPKSE
                     QGHLPPQAAVQGFNEIVAGISEKEARYEAQRCYSCGNCFECDGCYGSCPEGAILKLGK
                     GNRYEFNYQLCTGCAVCFEQCPCHAIEMVPES"
     gene            29567..30676
                     /locus_tag="PKM27_10240"
     CDS             29567..30676
                     /locus_tag="PKM27_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HNR07682.1"
                     /translation="MKSWYEIDDIDQLDSPALVVFPERVIYNIDQAIKMAGRASRLRP
                     HVKTNKSPEVCRLMMDRGITKYKCATIAEAEMLGMAGAPDVLFAYPLTGPKINRLVLL
                     IRKFPGTRYSALVDSLETAKTISAVLETEGLVFDVFLDINTGMNRTGMVPGDEAIDLF
                     HFCGREQSLRPVGLHVYDGHIRDEDLVVRKEKCDAAFERAVDLKDRLELENGDKLKMV
                     CGGSPTFPIHAARPEVECSPGTFVYWDAGYGTLFNEPYFLPAAVLVTRVISKPAPGLI
                     CTDLGHKAVAAENEIGKRVHFLNSRNLQALGQSEEHLVLKFTDEAGYKIGEVLYALPY
                     HICPTVALYEGVTIVENHRVTGYWRTIARDRKITV"
     gene            30701..>31719
                     /locus_tag="PKM27_10245"
     CDS             30701..>31719
                     /locus_tag="PKM27_10245"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNR07683.1"
                     /translation="MFIIDAHLDLSMNAMEWNRDLRLEIREIRRRERGLKDKADRGKG
                     LVSLPELRKGNIGLVVATQIARFVRPGSKLPGWNSPEQAWAQTQGQLAWYRSMEAEGQ
                     LRMIRDLKSLRSHLKYWSAERDPRDQSPKTIGYILSLEGADSLVDPAYLDLAYSYGLR
                     AIGPAHYGPGRYANGTDSRGHLNEAGRALLKRMQELNIILDATHLCDDAFWDAMDLFH
                     GPVWASHSNCRALVRHNRQFSDEQLKTLIRKNAVIGAAFDAWMIVPGWKRGISNPRDM
                     QCKADRVIDHIVHICMVAGNTDHVGIGSDLDGAYGQEQTPYDIRTIADLQRIPEKFLK
                     RGFRE"
BASE COUNT         7670 a         7991 c         8052 g         8006 t
ORIGIN      
        1 attcaccctc atcattgcgg tcccctctgc ggtaaaggtc ttcaactggc tgactacgct
       61 ttatggggga aatatccgtc tgactacggc tgccctgtac gggatcggtt ttgtgagcat
      121 gtttatttca ggggggctta ccgggatctt cctgggtaat tcagccatcg atgtgcagca
      181 acatgatacc tattttgtgg tggcgcattt ccatattgta atgggcgttg cggccatgtt
      241 tggcatgttt gcgggggtct accactggta cccgaagatg tatggccggt ttatgaacga
      301 aaccctgggt aaaatccatt tctgggggac catgatcgga gcctatgcga tattctggcc
      361 aatgcattat atcggcatgg ccggtgtgcc tagaaggtat taccgcttcg acacctttga
      421 agccttcaaa cattttgggg ccctcaataa gtttatgacc atagctgcga tcatcgtttt
      481 tgcctgtcaa atcctcttta ttatcaattt tttctacagc atttggaagg ggcgtaaggt
      541 gaccagcaga aatccctata atgccaatac cctggagtgg acgaccgaaa ccatcgttcc
      601 cgggcatggc aactgggaag gcaatattcc caccgtccag cgttgggcat atgactacaa
      661 taaagatgag agggaattca ttccccaata tgtacccgtc ggcccggatg aaaaggacga
      721 atcccattaa tcgcaccggt ataaatgcat tgacaaaaac ccgatggatt tgaaaaccgg
      781 cgaaacttat acgatatcct atgcgatgga tggcctgaag gccaaggtgg gagacctggg
      841 ccaattggtc aaacacaaac tgaccttttt tgtagtgctc agttcggtgg tcggctacct
      901 gctggcggcg acgggagaga tccagtggtt tcaagtggtc ctgcttgcct tgggtggctt
      961 tatgatcacc gggtcagcca atgccctcaa cgagatcatc gaaagggatt atgatgcctt
     1021 gatgacccgg acggccgggc gtccactgcc ctccggccgg atggaagtca cggaggccgt
     1081 aatctgggca ggcgtaatgg gcgttgcagg gatccttttg ctggcgttct tcaatctgat
     1141 cacggccgtt ttaggcgctg cctccctgat cctatatgct tttgtttaca ctccgctgaa
     1201 acggatcacc ccgctgtctg tggctgtagg ggccatccct ggcgccttgc ctgccatgat
     1261 tggctgtatc gccctggaag gaaaaatcac cctgctgtcc attaccttat ttggcatcca
     1321 gtttttctgg cagttcccgc atttctggtc cataggatgg cttggctttg acgaataccg
     1381 gaaagcgggt tacaagatca tgccgcttcc gtcccgggaa agggaccaca gcattggcat
     1441 gtacagcatg atatatgcct tgttcctgat cccgatcagc ctgatgtttt tgttctaccc
     1501 gggagtgagc aattatgcgg ttgccggcct gtgcctggcg gcttttggtt atgcggtggc
     1561 cgcctggggg ctttttatac acaatgatct ggccagggcc aggaaactgt tgttcacatc
     1621 cttgttttat ttacccatta ttcagatcat cttattgaca gataaatggc tctaacccaa
     1681 tcaggaccgg tcaaccgttt tggtatccac ccgcaaaaat ttgccttgtg ggcggccatg
     1741 ggaagcattg tcatgatgtt tgcttccctt acgagcgcct atgtggtgag gcggggtgcg
     1801 ggcaactggc ttgaatacaa attgcccttg ctgtttttca tcagtaccct tgttttactg
     1861 atctccagcc tgaccctgca ttggtcttat tcttcttaca aaaaaggcct ggaacttcga
     1921 tacaaatctt tcctcgtcgt cacgctgctt ctcgggctgg cttttgccgt acttcaatac
     1981 cagggttggc ttcaacttta cagttatggg attgaattga agggcaatcc ttcgggatct
     2041 ttcttttacg cgatttccgg catccatttg gcccatatcc tgggcggact tgcggccctg
     2101 acggtagcag gcattcatgc tttcggcttg ccctttgcag tcagcgaaaa aagacggcac
     2161 cggtttgaac tggtttgcca ttactggcat tttgtcgatg tgctctgggt atacctttta
     2221 acttttatga tcattcaaca ataattttta caattctgca tacatgacca cagatacaat
     2281 caaacacgga gcccatgaag atcaggccca ccaggaaggg ggcaatttat ggggtggagg
     2341 cagggaaccc ttcaaggcga gctatggaaa gctcatgatg tggtacttcc tgctctccga
     2401 cgcttttacg ttttccggat ttctgatttc atacggagcg ctccggttta gtatgcccac
     2461 ctggcctttg cccgaccacg tctttgaaac cattcctttt tattccggca aggcacccct
     2521 gattttcgtg acgttcatga ccttcgtgct gatcatcagt tcggtcacga tggttcgcgc
     2581 cgtccaggaa ggtcaccgcg aaaacaagaa gggcgtggtt ttctggatgc tgcttaccat
     2641 cctgggcggg atcaccttcc tgagctgcca ggcctgggaa tggacgacct tgctggccaa
     2701 agaacatgtg agcatccacc gaaatccttt cagccggcac atggaagccg gggtatacct
     2761 caatgaggat ggaaccccga tcgtgaaaga cggggccgaa gagaccttta aaaaaggcga
     2821 ttcctacctt attcacaagc acgatgggga gtgggacctt cgcaaatacc gggtcacgga
     2881 aggccctgac gcggggcagg taaaacagct tgccttcgga cccaaagcct tcggtgcctt
     2941 attctttttt atcaccggat tccatggatt tcacgttttt tcaggggttc tgttcctggc
     3001 catcatagcc atcaatgccg ccagcggggt atatgtggcg cggaaaaacg ggtatgaaat
     3061 ggttgaaaag atcggccttt actggcattt tgtggacctg gtgtgggttt tcgtgttcct
     3121 ggttttttac ctgctgtgaa acaggaccct cctttcattc attaatataa agaaacagaa
     3181 aagatatgtc agctcatctc agttacgaag aaagcaaaaa agtggtattc aagggcttga
     3241 tcctgcttgg ggtcatcacc ctgatcgaag tgtttattgc cctgttcggg aacggccacg
     3301 tcatttccgg ttttcacctg cccaaaatca tcatgtatcc catgatgatc ggcctgagcc
     3361 tgtacaaagc ttattttatt gtttacgaat tcatgcacat gcgttatgaa gtcaggggat
     3421 tggcgcttgc cgtcctgctt cccatggtgc tcctcatctg ggctatgatc gcttttttca
     3481 atgagggcaa cgcctggttc aactaccggg attatgtaag gacaaaaaac aaaacggaag
     3541 tcaaaacttc cattcaaccc cagggcgata aaaaacccgc ggaaaccctg gaaatccagg
     3601 aaatccattg agtccgaaac cggaaggcgg ggtgaaaaaa aacaggcttg ccttgtggat
     3661 ctccctcttt atcctgctgg ctttgcctgc catttccttg tactattctt ttcagggatc
     3721 cctgctgaga aaaaaatccc tggttgaact gagtccgaaa ggccgtctgg atcctggttt
     3781 ggccagcctg ctgagcggtg agtttaatta ccgggtcatt ggttccgcct gtgaggatgt
     3841 ggagcgcacc ggggccctga tcgatcagtt tgcccaggaa aagatccaat ttgtgattct
     3901 tcaggacagt gcttttgaac agcgttttcc ggcccgccag gtggagcatt ggaaaaaaca
     3961 aaacatcctt ctcattctcc ctgatcccgg acctcacctt cccggtgatc agaatgcctg
     4021 tacttttgta ctcgtaaata aaaattcgga gattttgaac cattataact tcacggagcc
     4081 ggttgaccgc cgcaaactgg tcgagcacct tgccttcctt gtgaccagta aataacgttt
     4141 ttgccgatgc gattacgcgc tgccaaaaac agtctctata ccctggccct ccgccttggc
     4201 ctggtttacg ccgcatatat gctgagccgg gtcatttttt acctcctcaa ttataagacc
     4261 tttgggttta tcccgcttgc ggaatggcct gaaatcatca tgggtgggct gctcttcgat
     4321 acttccgcca tcgcctatac caatatcctt tttattcttc tggccttatt cccggtaaag
     4381 gaaatcatca gcctccgttc gtatcaacgt ctttacgatg cggcctttct caccgtcaac
     4441 ggagcctgcc tgatcatcaa tgccgtagat tttttttatt tcaggtataa tcttaagcgg
     4501 atcaccgccg attttgccca ttgggccggg gaatcgaatg tgatgaggat catgatgagt
     4561 tttgcggggg aaaactggta tgtctttgtt cttgcggcgg ggcttttgta cctgttttac
     4621 aaatggtacc ggaggatacc attgtatgtc acccttttgc ccaatgcctg gttgtttggt
     4681 tttgtacatc tcctcttatt gagcggcatg acgggattga gtattgcagg catgcgggga
     4741 ggtttccttc attccaccag gcccatcacc ctgagcaacg ccggcaaata cgtgagccgt
     4801 ccggatttga tgcctgtggt gctgaatacc cctttcagcc ttatccggac ctttgacaaa
     4861 aaagcctatc cggagaagac atatatgcct gaagaagaag ccatccaggt atttaacccg
     4921 gtaaaaaatt attccgacag cctgcccttc gaggccaaaa atgtggtcat cctgattata
     4981 gaaagcctgt ccagggaaca ctccgggtat ttgaatccaa acctcggcgg cggggaatac
     5041 aaaggctaca ccccttttct ggacagcctg atgaaggaaa gcctggtctg caccaacgcc
     5101 tttgccaatg gccggaaatc cattgatgcc atgccgtcca tcctcgccgg catcccatcg
     5161 ctggtgctcc cttatgtctt gtcccataac agcctgaaca agatcaactc cctggcctcg
     5221 cttctgaagc ccaaagggta tcgttccgct tttttccacg gcgccccgaa cagttcaatg
     5281 ggtttccagg cgttttccag gattgccggt tttgaccagt attatggaaa ggacgagtat
     5341 gggaatgacg cggattatga cgggatctgg ggcatctggg atgaagagtt cctgcagtat
     5401 tttggcaggc agttggatac cttcacccct cccttccttg ggatctgttt caccttatct
     5461 tctcatcacc cttttaaagt gcccccccgg tatgaaggcg tattccctga aggaaaactt
     5521 cccgtacacg cctgtgtctc ctataccgat atggcattgc gtaaattctt tgaagtcgcc
     5581 tccaccaaac cctggtacag gaataccctt tttgtaatca ccgccgacca ttccacggtg
     5641 gcttggtccg aaaaatacaa cagcccggtt ggagcctttg ccattccgat cttgtatttc
     5701 agtcccggcg gcctgctccg gaaaacctac cagggagtga cccagcaaac cgatattatg
     5761 ccttccgtcc tggactacct gcatttcgac caacccttcg ttgcctttgg taacagcatt
     5821 ttcgatgatg aggcggaacc ctttgccctt cattacaata atgaacaata ccaggtcatc
     5881 gacagcgacc accggttgag tctttttgac gggaacaaga tcaccggtgt ttacgatatc
     5941 cgggattcct ggtccctggg tggaaacaag gtcctgttgc cggcagagag cgaatccgaa
     6001 ctgctccggt tccaccaggc agtcatccag caatacaacc aacgcatcct tcacaataaa
     6061 ctgacggtcc accaataaat accagccatg ccaaaatctc cggttcctga atacaatccg
     6121 gcccttgcaa aaaaattaaa cgggatagcc attgtttttt ccgtagtggt cgttctcatc
     6181 gtcgcttccc tgagacgttt ccatgtcgac accccggtcg atttccgctt tctggcccct
     6241 ctgcattcat cccttaacgc cctgaccgca ctgggtttgt tgctggctta ttatttcata
     6301 aaacagaaaa aaatcctttg gcacaagcgg gtcatgatcc tcaatatggt tttttccgcc
     6361 ttgttcctgg tgtcttacat tatatatcac ctgactactc cggagacccg gtattgccat
     6421 gaaggggtta tccgctacgt ctattttttt atactgatta cccatgtgat cctggccagc
     6481 ttcatccttc cggtggtctt gtttaccttc atccgggcaa tcaccggtca ggttgaaaaa
     6541 caccgcaatc tggccagatg gaccttcccg gtttggttat atatcgcggt ttccggccct
     6601 gtcgcttacc tgatgttaag aacatgcctg tgaaggaatt attgccggcc cccccgtata
     6661 tttgcccaat gaaacggacc ggtatcgttg taatgattgt attgctcctg gtgacggaat
     6721 ccttaatggc gcaatgtcca atgtgccgga tgtccctgga atcgaacctt gaagacggcg
     6781 gcacgatggg ccgcacgatc aatgccggca ttttgtatat gctggggatg ccttacctgc
     6841 tggttggcct gctgggctat atctggtata aaaaccggaa aaaaatggaa gccgggcagg
     6901 aagagcagac gaaccttaat tgacttctcc atctcctttt tagtttctta taccgggacc
     6961 ttcgaagttg actcgcctgg acaaagcaga tcgcgtcgtg gttctctcca tttaatattt
     7021 tatctttaag caatcggcct ggcattgaag tttggtgctt tccggtctcg ccgagggact
     7081 ttgttttccg ccatgatcct gaaaaataaa aatatcctgg tcattggagg ctccggcggg
     7141 ctcggcctgg cggcctgcct tgttttcctg caggaaggag caaatgtttt tgcgagcgga
     7201 ctagggccgc cggaagcatt gcccgtttcc tcctcaaatt tcacctacct ggtctcagat
     7261 gctgccaatg aagacgcggt ggatcgcctg gtgaaggaag ccctgacccg cctcccgcaa
     7321 atccacggac tttatcatat cgccggagga tcggggagga aggccggaga tggaccgctt
     7381 cacctcatga gccttgaagg atgggattat acgctcaatc tcaacctgaa gtccatcatg
     7441 ctgtcaaacc gggccatcat ccggtattgg atggataacc atttgcccgg ggtcatttta
     7501 aatatgggtt cggtactggc ctcctcaccc tccgctcagt atttttcaac ccatgcctat
     7561 gcggcggcca aagccggcgt ggagggcttt tccagatccc ttgcagccta ttatgccagg
     7621 tccaacatca gggtaaatgt catcgccccg ggcctgatcg aaacgggcat gtccacacgc
     7681 gcgcaggaag acccggccat ccgggctttt atcaaaacca agcagcccct ggacgggggc
     7741 agaatgggaa aaccggcaga ttgtgccggc acggcggcat ttttattatc ggatctttct
     7801 tccttcatca gcggagaagt cattcacgtg gatggcggat ggcaattatc tgatggacaa
     7861 tattatacca agtaattaat aaagcattag ttgaatagtg atcgccggaa ttgatatagg
     7921 agctaccaat ataaaagcgg tcgttttgga ccaagggtac gcgttggtgg ataaatttca
     7981 gggaccgacg aaggatgacg ggaaaaccaa atcctggatg gcagaggtga agaacatggt
     8041 ggataccgtc gtgaagaagt atccggacct ttcctgtatg ggtctttcag ctccgggtat
     8101 accggatgaa ggcaatacct gcatcagttt tatgccgggc cgtctctatg gacttgaggg
     8161 cctggtctgg agccaatacc tgggcattcc ttcctgcgtc ctcaatgatg ccaatgcgtc
     8221 catggtggcg gaaagccgaa taggggccgc aaaaggatat gaacatgcga tcatgctgac
     8281 cctcggcacc ggggtcgggg ggagtatact cattcatggc aaattgtaca ccggctttcg
     8341 acaggtgggg gggcacctgg gtcacctcag catcgacatg cacaattcct ttcgttccat
     8401 cacagggatg cccggcagcc tggaagacgc gatcggagat tcaaccgtaa aagccaggac
     8461 agggggcagg tatgcctcaa cgaccgaatt ggtaaatgcg gtcagggagg gggaggaaga
     8521 agcgcaaaaa gtttggcttg aatcggtgag ggcactggct atagctgttg ccgggttggc
     8581 aaatgtattg tcgccccagg ccctggtgct tggcggggga atcgcctccg caggcaacct
     8641 gttgatggaa ccctttctcc ggttcctgga cgaatacgaa tggaggccgg gtgggaaaaa
     8701 aatggaaatc aaaacagccc ggcttggacc gtttgcaggg gcaaccgggg caggtatctt
     8761 tgcttccgaa tatttcaaat catgagcctg accgaccaat acctggataa atgcctcgcc
     8821 ctggccgaaa gagtgagagc ccaacattcg aaaatcctgg aagcggcgga gtggtttgca
     8881 gggtccatcc tgaagggaag ggtggtccat ctctttggtt ccggtcacag ccggatcatg
     8941 gtggaggaaa tgtggcccag gtatggttca tttccgggat tcaaccccat tgttgaattg
     9001 tccatgacct ttcacaatct ggtcaccggt gccaatggcc agcggcaagc catgtatatc
     9061 gaaaacatgc cggggctggc ggaaaagatc cttcggaatt tcaacctgtc cgaattggat
     9121 accgccctgg tcatctcgtc ctcggggacc aatatcgtgc cttgtgagat ggccgaaatc
     9181 ttccgggaaa ataaaataaa agtggtttct atcgtcaccc tggatcatct ccaaaagagc
     9241 gaaagcaaaa ggtctgaccg gaaaaaactt accgatttca gcgacctggt tctggataac
     9301 ggcgcgcccg ccggggatgc catggtccgg atcgacggga tggaagcccc agtcagtccc
     9361 ggcagtacgg tcggttcagt attgctgatc aacagcatca aggccgaact ggcaaacttg
     9421 cttatgcttg gcggccagcc cccgaatgcc ctggtggcgg cttgcctggc cggggcggag
     9481 aaggcctccc atatctttaa tgaggcttac gatgagcacg ccacccggat gtcccgcctc
     9541 tataattttt acaaacctat tgaaataaga tgacccggac caatacctta agaaccaccg
     9601 tggtgggcag ctatcctttc cctgcctggc tggagtttgc ttccagaaat ctccatcagt
     9661 ttggaagcgc ggatatcgaa gaaatgattg aagacgcggt ccaggtggcc atacaggatc
     9721 agctggcagc ggacctggac gtcatcaccg atggtgaaca aacccgcatt gattttaatc
     9781 tgtcgtttta cgggtatctg gatggaatcg aacaggaatc ggaagcaccc aggaaatggg
     9841 gaccggccgc ccatgaccag cggggcaagc ataaaattac cggggccgtc agggcaaaac
     9901 atggcctggg cactgtggcc gaattcaaac gcctgcaaaa gctggccggt ccatcgggta
     9961 aagtgcttaa gtgcagtgta cccgggcctt ataccctcag cggccggctg gatcccaatg
    10021 gcatctatgc ggaccgatgg gaggttacgg aagcccttgt ccctatcgtt gctgatgaac
    10081 ttcggaagct ggtggaggct ggctgtacgg agataacggt ggacgaaccc tctatgagtt
    10141 gttatgctta taaagaagat ccccttcgtt ttgtggatat tttcaaccgg acggtgcagg
    10201 ccgtccgcgg aaaatgtttt ttatccaccc acctttgttt tggaaattac aaagggaggc
    10261 cggttggcaa aaggaccctg gctccgatgt tgccggcttt cctggacctg gccatcgacg
    10321 aggtgcatat tgaaatggcc aaccgtgaat tcgccgaaat cgaacgcttg tctgattttg
    10381 cgggcaggat gcgcgtagcc gtaggaatca ttgacgtaaa aaactattat atcgaatcgg
    10441 tagaggaggt cgtattcagg atcaaacaat gtctcaaatt tgttccggcg gatttattgt
    10501 cggtggctcc ggattgcgga ctcagccaaa ccgcccgctg ggccagccgt caaaaactga
    10561 agaatatggt gttgggagcc cgcgccgttc gggaagaaaa gggtttgtag aattacacga
    10621 tgataaaggc cgttcaaaag gatgatgcat tcttaaccga ccttcggggg gtgggagagg
    10681 gcagggcccg cctcgatatc tggtggcttg ggcaaagtgg gttcctgctg cactggaatg
    10741 gaaaaaatat tttattggat ccctatttgt cggattcgct taccctgaaa taccagggaa
    10801 cggccaaacc ccatatccgg atgtgcgaac gggtggtgga cccggcaaag cttcaattca
    10861 tgcattatgt gacgtcaagc catgtgcata cggatcacct ggatccggat accctggctc
    10921 cccttttcgc caacaatccg ggcctgaaac tcattctccc cctggctcat ctcagagaag
    10981 ggatgaaacg ctcgggttgt tccggggagc ggatgggggg actcgatgcg ggacaaacat
    11041 ttgctgaaga cgaaatttcg tttacggcgg tccccgcggc gcacgaaacc ctacaggtgg
    11101 atgaagaagg gcatcacctt ttcctgggtt atatcgtccg ggcaggcccc tggaccctgt
    11161 atcacagcgg cgacacggta ttgtatccgg gaatggccga attactgaaa ccctatgcac
    11221 cggacgtatc cttcctgccc atcaacggca ccgacccctc acgcggggtg tccgggaata
    11281 tgaatgcaag ggaagccgtc tggctggcga aaaatatcga cagcaaactc acaatcccct
    11341 gtcattttca tctatttgaa ttcaatacgg ttgaaccgga tgagttcatc gagacggccc
    11401 aaaaggaaca ggtacccatc cgtgtgatgc aggtggggga acgcctgagc ctgcaactct
    11461 gaatattaag ccttaatttt accctgcaga ttttcttttg tgaatattcc tgaaagtcct
    11521 ttaccccgga tcgtcatcgt gggagccggt tttgcaggcc taaaactggc ccggaggctg
    11581 gtggggagta agtatcaggt cgtactcatc gataagaaca actatcacca gtttcagccc
    11641 ttgttttacc aggtggccat ggccggtttg gaacccagta ccatttcttt tccgctccgg
    11701 cggatcctgc ggtcggatgc caaagttttt atacggatcg cggaagtgca ttccgtaaat
    11761 ccggaaagga atgaactgaa tacggatatc ggctccctga aatacgatta tctgatcctt
    11821 gccacagggg ccaaaaccag tttttttgga aataaaaaca tcgaggacat ggtcctcacc
    11881 ttaaaatcct tgtccgaatc gctgctggtc aggaatacca tcctggatca ttatgaaacc
    11941 gccctgacgg aaagcgacca cgaaaaaaaa cagggttatg tggacattgt gattattggc
    12001 ggagggccat cgggggttga actggccggt tcgctggcgg agatgaaaag atatatcctg
    12061 cccaaagatt acaatgaatt gaatccgaaa gaggtggata tttatctcat tgaagccaac
    12121 aaccacctgc ttccctccat gtccaaaaaa tcctcccaac gggcggaaag gtatttgcag
    12181 aaaatgggtg tactggtgat gaaaaacaac cgggtgacga atgtggacgc ggattttgtg
    12241 tatttgcagg acggttccag gatcatgagc aaaaatgtga tctggaccgc gggcctggaa
    12301 ggggcggtga tcccgggaat tccccgggag gacctcgtga aagccaaccg gatccgggtg
    12361 gaccggtacc agcaaacgcc ttcctacaag aatgtgtttg tggtcgggga cctcgcttat
    12421 atggaagaac ccgattatcc caacgggcat cctcaggtag cccaggtagc gatccagcag
    12481 gcaaagaacc tcgcttataa ttttaaacgg cttcatcaac ccggctatgt ctggaggcct
    12541 ttttcatacc gggacctggg caccctggca acgatcggca ggaataaagc cgttgccgaa
    12601 tttaaatggg tcacggtgca cggtttcatt gcctgggtcc tctggttatt tgtacatctg
    12661 ttccaattgc tgggagtgag gaacaaatta tttgtattca tcaactgggt ggccaattat
    12721 ctttcctatg accaggccct tcggtttatc atcaagccca aaaaagacaa acggtcagaa
    12781 ctgaaaaaaa gcaaggcctg atccccggcc cggcttttgc attaattttg accctttgaa
    12841 tggatatcca tttaattaaa aaggtaccgt atggcctgga attggctttc tgaccggaat
    12901 cccggttatc aaaaaatcgc ccaaaggctt gggctggatt atgaggaaaa ggatcaggac
    12961 aaagagatcc tgcgcgccca taagggatat catttattca aaacggggag caataagaaa
    13021 gtcaaatata tactcagcgg tttgctcgag tccaaggaac cgttccgggt ttttgaatac
    13081 agttttgcgg tttccacggg caaatcgacg acggtcttta cccagcacgt attgtccgta
    13141 aggttgaaaa aatcccttcc tccctttacc cttcggcctg aaaacctttt tcacaaaata
    13201 ggggaatggc tgggttggaa agacattgac tttgacgctt tccctgattt ttccaaacag
    13261 taccggctga tgtccggcaa agaaagttcc gtgcgcaaag tatttaatga ccatatcctt
    13321 gggttcctga gttttgaaaa aggctggtgg gttgaatgcg atggcccgac catcatctat
    13381 ttcaagcaca gtaaaagaat gaatgaagaa atggtggaac cctttatcca ggtatcccaa
    13441 accatgcatt atttcctcac cgggtttaaa gggatccggt gagaggtggg tggtggatgg
    13501 tggatggacc tcatacctct aacctcttac ctctaacctc aaccctcaat cctcaatcct
    13561 acgccggtat cgaaggcctg acctcgattt tgctgggcag ggtacgtggg tgcattttaa
    13621 gcaggtcgat gaccatttgg ccaatatctt ccggttgtat tttccagaaa tccttttcat
    13681 tggggatgtg attgttgaaa aaggtggcta ccgatccggg catgatggtc gtcaccttga
    13741 tctggtgcgg acggagatcg agcatgaggg cctgggtgaa gcccacgagt ccgaatttgc
    13801 tggcattgta ggcggtgcca tttacaaaaa aattggtccc ggcaaggctg gcgatggtaa
    13861 tgatatatcc gtttgatttt ttcagcgcgg gcaggctggc ctggacacta tggaataccc
    13921 cggtcagatt ggtatccaag gtatcgtgcc attgttcgtc gctcagttgg tcaaaggggg
    13981 cgaaatggcc aacgcccgca ttggcaacca ggacatccag tttgccccat ttttttatga
    14041 tttgttccac cgcctgaatt tcgtcttctt tgtttttaac atccgaccgg atcaggagaa
    14101 attgattggg cggaaagggt ttgtcgtcaa aatggtcgat gtgccttgcg ctgacggcta
    14161 ccttcaagcc cgcttcgagg gcttttacgg cgatccccag tccgattcct tttgtgccgc
    14221 ccgtgatgta gatcacttta ccgatgagat tttccatatt atccggttta ttcttaaact
    14281 aaaggtaagg gaatccccat aagaaaaagc cattccggaa tgccggaatg gccttattca
    14341 acaaaaccta tgacatatga ttagatcgac ttggcgaaag tcagatttac tttaaggtca
    14401 aattcatcct tgatcatctt gtcgccgatt tccgggaaga atctcccgct gcggtatttg
    14461 atatcaaaca gcgtgcggtc cactttaagg gacgcggagg cattgccttc ggattggttg
    14521 acggtggcat caaaagtgat ttctttactg atccccttga tgatcaggtc tgccgtgatt
    14581 ttataaaggt ttgcggattt ttgttccgct tttttaatca ccaggctgga ggtgccaaat
    14641 ttatcgacac tgaaaaaatc atcgtttttc aggtggctga gcaggcgttc catcatggat
    14701 ccttggatat cggtgacggt catggactgc atgtcgatat caaatctgcc cccgatcagt
    14761 ttgttcccgt taaactcaag agccccttgt tttacctgga gcgtacccgt gtgctggccc
    14821 gtcatcttat aagcggtcca ttcaatcttg ctgttgaccg gatccacttt gtaggcaacg
    14881 ggttttaccg gatttccggc caaagcgacg gctgccgtga aaacggccag gagggtagaa
    14941 taaaataatt tcataagagc gagtgatttt aaataatgga ttaattacaa aaaaacactt
    15001 tcttttcaat taatgttgaa aagagccctt caaataaatc ataaattttt cttaacggat
    15061 gctaagctca aagggcattc gcatcagggg ttttcctgcc tgttccttgg tccaggatgc
    15121 aaagcgtgca taaggcctga accaggcggt aagtttattt tgaataatgg attcggtgat
    15181 ttccggttct tttgaagtca gatcttccag aagttgctgg atgggatctt tcatagcggg
    15241 atattccagg accttgtatt catccagttt agccatgcgt acggcacagt ccagggcgtc
    15301 ctgcattccg ccaaggcggt ccaccaatcc atgttccagc gcttttaccc ccgaccagac
    15361 tctgccttgg gcaaccgctt ctacatcgtc cttatccata ttgcggccct tggagaccac
    15421 tgagagaaaa cggtcataga ccatatccgt ttgttgttgc gcgatcaggc tttcttcgga
    15481 agaccagggc atgaatgggg taaaggcggt ggtgaatttc ccggttctca cggtatccat
    15541 atcaatgccc agtttatccc ccatgagttt ggtcaggttt ggaatcataa agaatacgcc
    15601 gatggaaccg gtaatggtat tgggttcggc aaaaatactg tccgcgctgc aggcgatgta
    15661 atagcccccc gaggcggcat aatctcccat ggaaaccacc accggctttc cggcttcccg
    15721 gatcaggttg atctcatgga gaatattgtc ggaagccatt gagctgccgc cggggctgtt
    15781 aatcctgaga accagggcct tgattttatc atcctgcctg atttttctca aaatccggag
    15841 gtacctggcg gagcctacct gcccgttgtg gccttcccca tccaggattt ccccttcggc
    15901 gaagacaatg gcaatgcggc ctgccgtggc agcgggcgca tgggccggct ggtggtcaaa
    15961 ggactcaaag taccggttga ctgaaaagat ggagggacgg gcggtctccg aaaggcccag
    16021 gtcgttttga atcatccggt aaacatcatc ctggtaggcc agggtgtcgg ccagcttaaa
    16081 acggatggcg tcactggcgg aacgggaaag gtattcgttg gatatatttc tgaggtcaga
    16141 gaccgcaatg ccacggcttt cactgatgcc ttcgaggtgc aggcgataaa gatcctccag
    16201 gtattcccgg atttggctgc ggttttggtc actcatctta ttgaagcgga cgggttccgt
    16261 tgcgctttta aactggccgg cataatagac ctcgaagctg atgccggtct tatccagaaa
    16321 ttccttaaaa aaaggaatca tggccccaaa ccctttcagt tcaacgaccc cgtttggatt
    16381 gagcaggatg cggtccgcta ccgaggccat ggaataggac ccttcggtga aatattcccc
    16441 gtacgcatag atgggttttc cggaggcttt gaattcaagc agggcctggc ggatgagcag
    16501 agcgctggcc gaaccgagac tggaatagcc agaacggtag aggatgcctt ttatcttggg
    16561 gtcggaggcg gcgtgttgga tcgattgtac caggtcatgc aggccaatgt gctgctcgtt
    16621 gaggtcgaat acaccggtgg aacggacatt attagttttt tcaggataat ataaatcggc
    16681 ccggatttcc aggaagcccg gcttgtccaa tgcttttttg ccaggctctc cggccgaact
    16741 ggctaccacg gacagtatac ctagtccgat aaaaaatacc aggaaaaccg ccagcagggt
    16801 acccaggcag gatcccagga gtattttcaa aaaattcata aatggtgtct tatttcctgc
    16861 aatttaacag aagggccctg taatatgttt attttttcga ataatatgca gtattgttct
    16921 accaacgaca ggtattgaga tccaggggat tatccagtgg gtcaggaaaa aaggcccgat
    16981 ggaccggatc cggtgatacc gtgggcctta cccggagttt ttatcctgaa tttggaataa
    17041 ttctctaata ttgcgctgct tatcagattg caggtcacga atggatagtt ttacgcgaag
    17101 ttatgatcca gttttatgca cgggaagaca accacttgag gcagcttcaa caggccgcgc
    17161 cccgttgctg gatcaacctt catccgcctt ttgaacaaaa cgaactggaa tccctggccg
    17221 cctccaagga tattcccctg gattacctga ccgactccct cgatcccgac gaacgttcca
    17281 ggtatgagcg ctgggatgac aacctgcgtt ttatactgat ccagacgccc atcctcaacc
    17341 cggatgaaga ggaaaaccag ccgatctata tcacggtgcc catcggcttg ttgataagcc
    17401 ctgaatttat cctgaccatt tcggcccatg aaaacaccat cctcaaaaag tttattgatg
    17461 gcaaggtcaa aaattttgat ccttccgaca cccggtcctg catcctgcag atcctggacc
    17521 agaatgtctg ggcttacctg gacagcctca agcggcttaa cctgaaacga aacctgattg
    17581 aacaggaatt gtacgattca agccggaatg cggaactcaa acaactgctc cggattgaaa
    17641 agagcctggt ctattttgtg aattccctca gtgccaatga cctggtcaaa ctcaagatga
    17701 aaaggacgga ttttctgcag atcgcccagg acgaagaaaa ggcggatttg tttgaagaca
    17761 tcctcattga caacaaccag gccctggaca tggccaaccg gcacaccaat atcctgtcgg
    17821 ggacgatgga agcctatgcc agcatagtgt ccaataatct gaataagttt atccagcggc
    17881 ttacggtggt cacgatcatc ctcatggtac ccactttggt agccagtttt ttcggtatga
    17941 atgtccacct gccgctgtcg gataaaccca tggctttctt ttatatcctc tttgtgtcgg
    18001 ggctgatcat tgtattcctg gtttggtttt tccggcgaaa acaaatgttt tgatttttgc
    18061 cgctttttct ccatccccgg gggaatggaa accggaattt taaacaataa aagggcggtt
    18121 actaacaatt tatttgactg taaatatcag atttacagct tattgcatag gttatccaca
    18181 ggctgttggt aattttaaaa catattaaaa aaatgattca tttatttagc agaatacttg
    18241 atgaatattt ttaatgtgtc ttaaagaagt tcatacaaaa aatattctgc aaaccgaatc
    18301 gaattgaaaa aaaaataaaa aatacttata taaaaattat ttatatttaa tttttataac
    18361 cggagaagtc gattattttt gagccgcaga caggatgccg atcataccct tcggatttct
    18421 atctgtaatc cataataaat catatttatt aatcctattc gtgtaaactg tataagccag
    18481 tatggaacaa cgtgtcgaac cacttttgca ggagaatcca aaccgttttg tcgttttccc
    18541 cctgcagcat aatgatatct ggcagtttta taaagattcc gtggcgggtt tctgggtcac
    18601 ggaggaaatc gacctggctc aggacatcat cacctggcag gaaaagctca cggaggatga
    18661 gcggcatttt atcaaacacg tgctggcttt tttcgcagcc agcgacggga tcgtgaatga
    18721 aaacctcgcg gaaaacatgc tccgcatggt ccagtacccg gaagccaagt ttttctacgg
    18781 gtaccagatc atgatcgaaa acatccattc ggagacctat tccctcctca tcgataccta
    18841 tatcaaggat ccttcagaaa aggattatct gttccatgcc attgaccaca ttccctgcgt
    18901 aaggaaaaaa gcggattggg ccctacgctg gatttccaac gggaccttcc agcagcaact
    18961 gatcgccttc gccgcagtcg aagggatttt tttcagcggt tccttctgct ccatattctg
    19021 gcttaaaaaa aggggattga tgcccggact caccttttcc aacgaactga tctccaggga
    19081 tgaaggccag cattgcgatt ttgcctgcct cctttacaac aaacacattg taaataaatt
    19141 gcccaaagcg gaggtcgaag agatcatccg ggacgcggtc accatcgaaa aggaatttgt
    19201 cacggatgcc cttcccgtca aactcatcgg gatgaacgcc gacctgatgt gccagtatat
    19261 tgaatttgtt gcagaccgcc tcttattggc cttgggcaat gagaagatat ataaggtgga
    19321 taaccctttc ccctttatgg aaatgatctc cctctccggt aaaaccaact tttttgaaaa
    19381 gagggtaggg gaatataaaa aatcggggat cgggaccacg cgggaaaacc aggtgtttag
    19441 tgtggatacg gaattttgaa ataggttaag ggttaaaggt taagggttaa gggagagaaa
    19501 agaggggaga agctctcgca ggtaccggca aagatcggta atttggagag ttgaactgaa
    19561 tggggtggcc gatggcggag gcgccaataa acttgaagaa aactattgcg atataaatca
    19621 ttaaaagaag ctacatgaag gtcttaaaaa gaagcggtaa gcaggaggag gtgtcttttg
    19681 acaagatcac agcccgcata aaaaagctgt gttatggcct caattccact tatgtcgaac
    19741 ccatcgaaat cgccaaacgg gtcattatcg gcctctatga cggggttaaa accacggacc
    19801 tggacgtact ggctgcggaa actgcagcgt ccctggctac ggtccacccg gattatgccc
    19861 tgctggccgc ccggatcgcg gtgtccaacc tgcataagga gactaaaaaa tctttttcgg
    19921 agaccatcca tgacctatac acttatattg accctaaaaa cggccagcct gccggcctga
    19981 tcggcaaaga aacccatgac atcgtcatgc gccacgccga ccggctggat tccgccatca
    20041 tatatgaccg ggattacagc ttcgattatt ttggattcaa aacgctggag aggtcctacc
    20101 tcctccggat ggacggccag gtggtcgaac gtccccagca tatgctgatg cgggctgcgg
    20161 tggggatcca tggggaaaat ctggaagccg ccattgaaac ctatgagctc atgtcggaaa
    20221 aatggttcat ccacgcaacc cctaccttat tcaatgccgg cacgcccaaa ccgcagttgt
    20281 catcctgttt cctgcttagt atgacggacg acagcatttc aggcatattc gaaaccctgg
    20341 cccggtgcgc taaaatttcc caaagtgcgg gaggagtggg gctcagcatt cacaatatcc
    20401 gtgcgcaggg atcctatatc aaaggaaccg ggggcaccag caacgggatc atcccgatgc
    20461 tgcgggtcta taacgatacc gcccgttatg tggaccaggg cggaggcaag cggaaaggcg
    20521 ctttcgccat ctaccttgag ccctggcacg cagacgtttt tgattttctc gaattgaaga
    20581 aaaataacgg caaggaagag gtgagggccc gcgacctctt ttatgccctg tggatccctg
    20641 acctttttat ggaaagagtg aaagcggacg gcaactggtc tttgttctgc cccaacgagg
    20701 ctccgggact ctatgatgcc tacggcgccg atttcgaaaa attatatacc caatatgagg
    20761 cccagggcaa ggcccgcaaa gtgatccggg cccaggacct ctggttcgcc atcctggaat
    20821 cccaggtcga gacgggcact ccttatattc tctacaaaga cgcggccaat cagaaatcaa
    20881 accagaaaaa cctggggacg atccggtcca gcaacctctg tacagagatc atggaataca
    20941 cctctcccga tgaagtggcg gtctgcaatc tcgcctccct gtccctgccc aaatacgtga
    21001 aggcgaacaa agaatttgac cacaaaaaac tgatggagat cacaagggtg atcacccgca
    21061 acctcaaccg gatcattgat atcaattatt atcccatcga ggaagcgcga aacagcaacc
    21121 tgaggcaccg acccatcggg ataggggtgc agggactggc cgataccttc attctcatgg
    21181 gttatgcctt cgacagcccg gaagcccgtc aactcaatcg cgagattttt gaaaccatct
    21241 attacggcgc tttgctggag tcctgcacgc tcgccgaaaa agacggggct tattccagtt
    21301 tcaagggctc tccggccagc cagggcatcc ttcaatacga tatgtgggag gtgaccccga
    21361 ccgaccgttg ggattttccc gccctgaaaa aacaaataaa aaaacacggc ctccgcaaca
    21421 gcctcctgct ggcccctatg cccacggcgt ccacctcgca gatcctgggc aacaatgaat
    21481 gttttgaacc ctatacctcc aatatataca cccgcaggac cttgtcgggc gagtatgtcg
    21541 tagtcaacaa acacttgctc aacgacctgg tcaacctggg actgtggacc aaggagatcc
    21601 gggaaaagat catcgcttcc aacggctcga tccagcacat catggagatc ccggccacga
    21661 tccgcgacct ttataaaacg gcctgggaac tcagccagaa agtcctgatc gatatgtccg
    21721 ccgaccgggg cgcttacatc tgccagagcc agagcctcaa tctttttgtg gaaaatccca
    21781 gtttcgccaa attgtcttcc atgcattttt acggctggag caagggcctt aagacaggaa
    21841 tgtattacct gcgcagcaaa gccgccgtcg atcccattaa attcaccctc agcgaagatt
    21901 atcagaaaaa atttgtgacc aaggaaccgg atccccggat gacggtaagc gacctggacg
    21961 agcaggacca gcctgccggc cgtgcagcaa ggccccaggg cgtgccctcc gtagccccga
    22021 gagagaccgt agccgcactg gtggatgaac cggtcatcgc ggccggtata aaagcctgca
    22081 gcctggatga tccgggttgt gaggcttgca gcgcctgacc acaagctcat ttctctgtct
    22141 taaccacaat ttgatgctta attctttgaa tgagattgaa tgtggttttc ctatcaacag
    22201 actttaagga ctgtaacatt cctgaaactg gatacgatga attaaaaaag ataatatcgg
    22261 ctgtgaagcc ggtggtggac acgtctttat aaaaggaatg aaagatttct tgattcatta
    22321 agcgaaataa aaatattcaa atcaataagg ccatggatag ctcattccgg aatttcaccc
    22381 ggtaccttct gcctaaaaag tttgccttga ttcattggta aaaaagtcct atttttgtag
    22441 acaaaagtct acatcttgat tgcacaagcg gaagcaggca tattgacccg gttggagaaa
    22501 atactccatg attttattca ggaattaacc cctgcaaagc ataaaaagcc gctaaaaatc
    22561 aaggaaatcc tggatgacaa aagactggtt atgttgatga tccgtcacgg acttccttat
    22621 tccttttata cccttatcga atccctcgca cctttcaacg agtcggactg ggcggcttat
    22681 tttggcattt ccaccaaatc cctgcagcgg tataaaaaag ccggtgagcg ctttaagccg
    22741 ctgcagtcgg aaaagatcat ggaaatggct gaggtgatct acctgggcct tgaattgttc
    22801 ggttcggcgg ataaattcaa tgcttggctg aatcaaagca acctggctct gggtggcgag
    22861 aagccggttg acctccttaa agattcctac ggtaaatcat tggtgaccgg agaacttacg
    22921 cggataagcc atggcatttt tgtgtagagg ttatttccta agacttacga acccgaggac
    22981 ccctaatgga agtataccgt ttgtgcagga aaatctactc cgggtccctg agcggcaagg
    23041 gcgccgcgct tgaaggggcg aggtggaatt ctccaggtat tgaaatgatt tatacagcca
    23101 tgaaccggtc cctggccatg gtggaggtgg ctgtacacct taatctttcc gccatgccag
    23161 atgattatta tatcctgacg atccatatcc ccgatgaact gtcattgaaa aaactcaacc
    23221 tgtccagtct gccggatcac tggaatgatt ttccttatca ccgggaaacc caggcaatag
    23281 gggatgcatt tataaaatcc ggcaggtatt gcctgctcca ggtcccctcc gcagtgaccc
    23341 gtggtgattt taatttactg atcaatcctg tacatccgga attcaggcgg atccggatcg
    23401 tggagcgcga acattttcct tttgataaaa ggtttatata agatgcaggc cgtgcggttt
    23461 gagcgatgag ccggggaatt ttgtgatact tttagtggaa gacaggtcag aatgtaacca
    23521 tttgttcatg gttccattag tatactccca ctatccaccc caaaatattt ttccctctca
    23581 taccagaata cccctatctt tgccccgtga ttgtaaaaca ctagcttccg tactccggat
    23641 attttatttc ttcccctctc cggttagtta ttccttatcc aatttataga ttgctttttt
    23701 tcttatcgtc acagtatttt tttaattttt taatttttgt attttatgaa catttatgtt
    23761 tcaaatctgg gttatcaagc caacgatgag gatttaaaat cccttttttc agcattcgga
    23821 cccgtggtct ccgcaaaggt gatcatggac aagatcaccg gcagatccag aggatttggt
    23881 tttgttgaaa tgaccaatga agcggacggt aaaaccgcaa tccaaaatct taacaaccag
    23941 aatgtagaag gacgttccat ctccgtctct gtcgccaggg aaagggatgc atccggcaac
    24001 tccaatatca acaggagctt cggaagtaat tccaattccg gaggctacaa gaaaaggtgg
    24061 taaacaaacc aaagggttgt gatctctaaa ggacacaacc cttttttttc agcttttggc
    24121 tcatcccgca atccaccact caccatccac catccacttc atcagtgcac cggttgaaaa
    24181 tccttcgcac tgaatcccgc catttcttca taattccgcc atctcagatc gacgatttcc
    24241 tgagccagtt gcatcaggtg ctcggcctct accggattgg atttggtaag cactttatat
    24301 cgcaattcct ggtaggcata gtcttttaag gacatggatg gccgcaggct atccagaata
    24361 aatggatttt gatccgcctt ccgcaacatg gggttgtacc ggatcaatgg ccagtagccg
    24421 ctttgcacgg catttttctg ctggttgagc cccttttcca tatcaatgcc gtgggcgatg
    24481 cagtgggagt atgccaggat cagggaaggc cctggataag cctcggcttc gcgaagggcc
    24541 agcaaagtct gctggggatt ggcccccatg gcgatctggg cgacatagac attcccgtag
    24601 gagatagcct gcatggccag gtcttttttg cccacccgct ttccggcggc ggcaaatttg
    24661 gcggaggctg ccgtgggagt cgctttggac atctggccgc cggtattgga atacacttcg
    24721 gtgtccaata cgatgatatt cacatccttt ccggtagaca atacatggtc cagcccgccg
    24781 gagccaatat cgtaagccca tccatcccct ccgatcaacc aaacgctgtg tttgatcagg
    24841 tgatccgcca cagagcccaa ctgaatggct tcccggctgt tcataccctg cagtattttt
    24901 ttaagttcag cgaccaaacg gcgttgcgtt tgtatttccg tatcgtttga ttgtttgtta
    24961 tccaggatgg attggacccg ctccacaccc accacgtctt tcattttcca aagaaggtcc
    25021 ctggccattt cgccctgttt atccaggctg atccgcatac ccaggccaaa ttctgcattg
    25081 tcctcaaaga gggaattgga ccaggcagga cccttccctt ctttattggt cgtccaaggg
    25141 gtggtgggga ggtttccgcc gtatatggat gaacaacccg tggcattggc caccaccagg
    25201 cgatcgccaa agagctgggt gatcagcttt atatacgggg tttccccgca tccggcacaa
    25261 gcccctgaaa actcgaacaa gggttccaga aactggacgc cgtgcaccgt ggagaaattg
    25321 aacttcgtcc ggtcattctc gggcagagct tcaaagaagg cgatgttttc cctgtcccgg
    25381 tccatgtcca aacgttcccg ccggggtttc atattaatcg cttttacact ctgatctttc
    25441 gggtcgtggg ccggacagac ctgaacacaa aggctgcagc cggtgcagtc ttcggaatag
    25501 atctgcaggg taaaccgggt gtccggaaaa cctttcgcat tgatgggcgc cgactggaaa
    25561 tgttccggct gatcttccag gaagccccgg tgataaaact tggaccggat cacgctgtga
    25621 gggcatacaa aactgcaatt gccgcactgg atgcagaggt catcccgcca cagcggtatg
    25681 ctttcggcga tattccgttt ttcccatttg gtggttccgc tggggaaggt gccgtccacg
    25741 ggcatcaggc tcacgggcaa ttcatcgccc agtcccatca tcattcgcgc ggtcacctgg
    25801 cgaacaaatt caggcgcttc gtcggtgagg gaaaacactt cttccgcggt tttggtcccc
    25861 gtgtcccggg caggtatctt caattcaaag aggttgctca gggactgatc gacggcttcg
    25921 aaatttttct gaatggtttt ggcccctttt ttggaatagg ttttttcaat ggctttttta
    25981 atgtgctgaa tggcttcttc acggggcaac accccggaca aagcaaagaa gcaggtttgc
    26041 atgatggtat ttatccggcc ggccataccc gtttccaggg caacgcggtt ggcgtcgatc
    26101 acaaaaatct tcatttcttt ttcccgggct ttcacctgga ccaggcgggg caaccggtcc
    26161 cagacctgtt cggtggggaa aggggcattc aacaagaggc ttgctccttg acgggcatat
    26221 ttcagcaggt cgtccttttc cagaaaatta aactggtggc aggctataaa atccgcctgc
    26281 cggatcaggt aaggcgcaaa aatgggcttt ggcccaaagc gcaggtggga gaccgtcttc
    26341 gctccggatt tttttgaatc gtagacaaaa taaccctgtg caaacaagtc cgtattttcc
    26401 ccaatgatct taatggtgtt tttgttggcg cccaccgtac catccgcacc cagtccgaag
    26461 aatagggcct ggctccagga ggattcatcc agggtgaacg tttcatcgta gtcgaggctg
    26521 gtatgactta catcgtcccg gattccaatg gtaaaatgat ttttaggatt ggattttgtc
    26581 agctcgtcaa atacggcctt caccattgcc ggggtaaatt ctttggagga taaaccatat
    26641 cgtcccccga tcgttttcgg caggcggccg attttttctg aagcataggc ttcgtttaaa
    26701 gccaccacca catcgttgta caaaggttca ccggaagccc cgggttcctt ggtccggtcc
    26761 agcacggcca gggagcggca gcttgcgggc aatgcgttaa gcaaatgttc cattgaaaaa
    26821 ggccggtaaa gccggacctg aatgacccca agtttttccc cggccgcatt cagatgcaga
    26881 acggtttccc ggatggtatc tccggcggag cccatgacca cgataatcct ttcggcttcg
    26941 ggatgaccgg tatattgaaa cagatggtag gaccttccag tcagttcatc aaaacgggcc
    27001 atggtctttt ccatgatgtc cggcgtgtct gaataaaaac ggttcaccgt ttcccttccc
    27061 tggaaataga catctggatt ctgggccgta ccgcgtatga aaggcttttc cggattcagg
    27121 ccgcgctgac ggtgccggat caccaggtcc tcgttgatca tggtcctcag atcctcttcc
    27181 gaaaggattt ccagtttatt gacttcgtga gaagtcctga agccgtcaaa aaaatgaatg
    27241 aagggtatcc tcgattccag gctaaccgcc tggctgatca gcgcaaaatc gtgggcttcc
    27301 tggacattgg cggaggagat cattccaaaa ccggtcatgc gggcggccat gacatccccg
    27361 tgatcaccga agatggacag gccctgggcc gctaaagaac gggcggcgac atgaaaaacc
    27421 gccgcggtaa gttcgccggc aatcttgtac atgttgggca acatcagcag caaaccctgg
    27481 gatgcggtga acgtagtggt cagcgcaccg gtttgcaacg agccgtgcag ggtgcctgcg
    27541 gccccgcctt cgctttgcat ttctatgacg tccggaactt gtccccagat gtttttaatt
    27601 ttttttgcag accattcgtc agccagttcc gccatggtcg aagagggcgt gatggggtaa
    27661 atcgcacaca cctcgttgat ccggtaggca acgtgggcca ctgcctcatt gccatccatc
    27721 gtggcctgca ttctttcttt tgctttcatt ttatttgttg tttcaggatt caggaaccat
    27781 ttcaatcgcg tggcagggac attgttcaaa gcataccgcg cagcccgtac acagttgata
    27841 attaaattcg taccggttgc ccttgcccag tttcaggatg gccccttccg ggcaggaacc
    27901 gtaacagccg tcgcattcaa aacaatttcc gcaagaataa caacgctgtg cttcgtagcg
    27961 cgcttccttc tccgaaatgc ctgctacgat ttcgttgaac ccttgtacgg ctgcctgggg
    28021 aggcaggtgg ccctgttcgg acttcggcgc ctccgtatcg taccaaaggt gaaggctttc
    28081 atagctgatc agcggatggg ccggtggttt gtgatattct tccccgcgca ggaaagcatg
    28141 gataaaacga gcggcctttt tcccatggcc caccgcaacc gtaaccgtgc gttcgctggg
    28201 gaccatgtcc cctcccgcaa aaataccata ggcgccggtc atcatattgg gcccgaccac
    28261 gaccacgcct tcatcattaa tatcaatgcc cggcaccttt ttaaggaagg cggtatcggt
    28321 ctcctgtccg agagccagga tgagggcatc ggcttcgatg gtttcataaa ccccggtacc
    28381 caccggtttg ccctcctcga tgcgcatgac ttctaccgtg aatgaagtct cggtaatctc
    28441 tttgatggaa cgcagccaat ggatctgcac cccctccgac aaggcttcat cggcttcaaa
    28501 agcatgcgca ggcatgtgtt cccggtcgcg ccggtaaatg atcatggctt catctgcacc
    28561 cagtcgtttt acggtgcggg cagcatccat ggcagtattg cctccgccat aaacgaccac
    28621 tttacgcccc agtttagggg cattgccggt ttcgacatcc ttcaggaaag tcaccgcgtc
    28681 caggatcttg cctgcttccc gtgcagggat ttttactttc ttgccaatat gcgcgccgat
    28741 ggctacaaaa accgcatcaa agccgcccgc ctttttctcc gcgacgatat cagatacttt
    28801 gtaatttaat ttgaaacgga ccccgagcgc ttccagccgt ttgatctcgg catggatgat
    28861 gttcctcggc agccggtaag ccgggatgcc aaaatgcatc attcctccgg ccatgggccc
    28921 cgcttcatat accgtgacct catggcccaa ccgggcgagg tgacaggcgg cggaaagacc
    28981 gcttgggcct gcaccgatga tcaatacctt gtggccggat gttgactccg gggccataaa
    29041 ttgccagttt tcttcgatag cccggtcgcc taaaaaacgt tcaatggagt gaatgcttac
    29101 gctggtatcc gtttgtcccc ggttgcagtg gtcctcgcaa ggatggtaac atacccgtcc
    29161 gtggatggcg gggaaaggat tgtcctcggt cagtttcagc caggcttcat aatacctggc
    29221 ttcctggacc agtccaagcc aggcctggat gttttcccct gcaggacagg catggttgca
    29281 gggcggcaga tagtcctggt aaaccggctt tctggtccgt ttagggccaa tgcccatgat
    29341 gtctttactc aggtccgcgc ggggagtcaa ctctttgttt ttcataaact aaaattagaa
    29401 ctaaactcta ttttttctca tccacccata ctgactactg tcattcccag gcttgactgc
    29461 tattatggtg ggcaggccca ataaatatgc aaggccttga ccctttcttc tctttagtcc
    29521 accgtaaggc gaaaagattc tgatggccgg aggatatctt tacagcatga aatcatggta
    29581 tgaaattgac gacattgacc aattggacag ccccgccctc gtggtcttcc ccgaaagggt
    29641 catttacaat attgatcagg ccataaagat ggccggcagg gcaagccggc tgagacctca
    29701 tgtcaaaacc aataagtccc cggaagtatg ccgcctgatg atggaccggg gaataaccaa
    29761 atacaaatgc gccaccattg ccgaagcgga gatgttggga atggccgggg cgcctgatgt
    29821 tttatttgcc tatccgctga ccgggcctaa aattaatcgc ctggtcctgc tcatccggaa
    29881 atttcccggg acccggtatt ccgccctggt cgattcttta gaaacggcca aaaccatttc
    29941 tgcagtactg gaaaccgaag gtttggtctt tgacgtattc ctggatatca atacagggat
    30001 gaacaggacc ggcatggtcc ccggagacga agccatcgac ctttttcatt tctgcggccg
    30061 cgaacagagt ttacgcccgg ttggcctcca cgtttatgat ggtcatatcc gggatgaaga
    30121 ccttgtcgtt cggaaagaaa aatgcgatgc cgcctttgag agggccgtcg atttaaagga
    30181 ccggttggaa ttagagaatg gagataaatt aaaaatggtt tgcggaggtt cgcccacttt
    30241 tccgattcac gccgcccggc cggaagtaga gtgcagcccc ggaacctttg tttactggga
    30301 tgcaggttat gggaccttgt tcaatgaacc gtattttctt cctgcagcgg tcctggtcac
    30361 ccgggtcata tcaaaaccgg cgccggggct gatctgcacc gacctgggcc ataaagccgt
    30421 agcggccgag aacgagatcg gcaaacgcgt gcattttctg aatagcagga accttcaggc
    30481 cctgggtcaa agtgaagaac acctcgtctt aaaatttacg gacgaggctg ggtataaaat
    30541 cggggaggtc ctttacgccc tgccatatca tatttgccca acggtagccc tttatgaggg
    30601 ggtgaccatc gttgaaaatc accgggtgac cgggtattgg aggaccatcg caagggaccg
    30661 gaaaatcacc gtttaattca catcctgatc ctgatatctt atgtttatta ttgatgcgca
    30721 cctggacctg tccatgaacg cgatggagtg gaaccgggat ttacgattgg aaatccggga
    30781 aatccgcaga agggaacggg gcctgaagga caaggcggac cggggaaagg gactggtatc
    30841 ccttccagaa ctcagaaaag ggaacatagg cctggtggtg gccacccaga tcgcccgctt
    30901 tgtacggcca gggtcgaagt taccgggctg gaattcaccg gaacaagcct gggctcaaac
    30961 ccagggacag ctggcctggt acagatccat ggaagcggaa ggccaactcc gcatgattcg
    31021 cgacctgaag tccctgcgca gccatctgaa atactggagt gcggaaagag acccccggga
    31081 ccaatcccct aaaaccatag gctatattct ttccctggag ggcgctgatt ccctggtgga
    31141 tccggcttac ctggacctgg cttattccta cgggctgagg gccatcggcc cagcccatta
    31201 cggaccgggc agatatgcca acggtacgga ttcccggggc catctgaacg aggcgggaag
    31261 agctttattg aaaaggatgc aggaattgaa tataatcctg gacgccacgc atttgtgtga
    31321 tgatgcgttc tgggatgcca tggatctttt tcacggtccg gtatgggcca gtcacagcaa
    31381 ctgcagggcc ctggtccggc acaaccggca attttccgat gagcaattga aaacattgat
    31441 ccggaaaaat gcggtgatcg gcgccgcctt tgatgcctgg atgatcgtgc cgggctggaa
    31501 acgggggata tccaatccaa gggatatgca atgcaaggcg gaccgggtga tcgatcatat
    31561 tgtccatatt tgtatggtgg ctggaaatac ggaccatgtg ggcatcggtt ccgatctcga
    31621 cggcgcctac ggccaggaac aaacccctta tgacatccgg accattgccg acctgcaaag
    31681 gattccggaa aaatttctca agcggggttt ccgggagga
//
LOCUS       DAOQXT010000050        31623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1683_length_31623_cov_6.723581, whole genome shotgun sequence.
ACCESSION   DAOQXT010000050 DAOQXT010000000
VERSION     DAOQXT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 31623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31623
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1683_length_31623_cov_6.723581"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..213)
                     /locus_tag="PKM27_10250"
     CDS             complement(88..213)
                     /locus_tag="PKM27_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07684.1"
                     /translation="MPVLQSVMSYIVMSRSSQNSVLDFSLDFQAEISSRPSGLVI"
     gene            complement(800..872)
                     /locus_tag="PKM27_10255"
     tRNA            complement(800..872)
                     /locus_tag="PKM27_10255"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:Phe,seq:gaa)
     gene            complement(1099..2004)
                     /locus_tag="PKM27_10260"
     CDS             complement(1099..2004)
                     /locus_tag="PKM27_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNR07685.1"
                     /translation="MKRRNFLKSGILASGILPSFHSNAIPGIADPVFKMKFSPDFGLF
                     GDLAGDNPVDQIKWGHDQGFRAWENTMLKNRPVEEQERISKTVQGLGMEWGQFVGTLT
                     FKDVTFAGRDGGLREKVMEDVRASVDIAKRMNTRFIHNVLGMADPKLPWDFQMANAVE
                     LLKRIAGVYEPHGLIMVMESMNHKIDHPGMFLHTIPQAYALAKAVGSPSVKVLFDFYH
                     VQIQEGNLLPTLDYAWDEIAYIQIGDTPGRKEPTTGEINYPKVLQHVYDKGYRGFVGL
                     EHGSSKPGKEGEVAVLAAYRAVDPK"
     gene            complement(2045..5179)
                     /locus_tag="PKM27_10265"
     CDS             complement(2045..5179)
                     /locus_tag="PKM27_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR07686.1"
                     /translation="MNFHHKNLYSCLFLLLASALLTLSQCGKSPDAATRDIEVPEGFT
                     IEVAAGPDLVDYPMFAFVDETGRLFVYESTGNVYNKTEDALKNPQFRIKLLVDEDGDG
                     FYDKYTVFADKVGFPQGGVFYKGSLYASSAPELLKLTDTDGDGVADQREVLLSGWELN
                     VNANSLIGPFMAPDGWLYLTSAIEGFDIMTREGEHLKGETARVWRLKPDGSGLQWISA
                     GGMNNPVGLAFTAASEVLGTQTYFTEPKAGLRDALMYWTEGGVYPKPNNNITRDRLPL
                     TGDLLPVVTKYSRVAPAGIIRYRGSVLDESYRDNLFSAQFNTHRVLRHQLIREGASFR
                     TEDEVFFSSENEDFHPTDVLEDGDGSLLVVETGGWFIKGCPLSQVSKPELKGSIYRVR
                     KKDAAKMEDPFGNHIEWAGLDVATIARYIEDARPFVADRAVNQLVDRSQESVASLAAL
                     LTNSPDAHARTRIVYALYRIHSSESIAALREGLKDKDLQVRVAAAKALGLAKDQGSVD
                     QLIQAIQTDLPAVRRQAATAIGQIGEPRAIPALLQSADGVKDRFVEHAIIYSLLSMNR
                     PDLVKAGLTHPSDEVKRAALIALDQMQNQSLKLEQVTPFLASADSMLESAARWVISHH
                     PEWAPDFIVYLQKQLNKPEWSEADKTQLKDILVSFCGDGTMQRFMADQMAAPQIEKKL
                     FVLESMSGCNIKEFPAAWIGPLGKELTSNAEVEVKLKVLKLIRLYGVSSLDHTLKQVA
                     GNKQNPAILRIHAIGTSLRDSALLTSPHFDYLYTQIQPDIEASIRQQAAGVLGQAKLS
                     DDQLLKIAKEFLGKADAFILPRLMPVFQGAKNLEIGTALAGALERSPGLDNFTEEYLH
                     GLFSNYPPELNEAKDQLMAKLSAVQGERLSLIHAIENSIVKGDIVRGRELFLGKAICY
                     TCHVIGNEGAGFGPDLTSIQRDRSAHDLVEAIVYPGVSFVREYETYRISTKEGTFTGI
                     IQEQTGDMIRLGISPQESVQIQRSDILSTEILEHSMMPAGLDKLLTEQEMADLLAFLI
                     GQDQDPETDARLLR"
     gene            5616..5789
                     /locus_tag="PKM27_10270"
     CDS             5616..5789
                     /locus_tag="PKM27_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07687.1"
                     /translation="MKLKIRFFQLHYGICFIFCAFCMHLSPGTRSLPGESMIKAEWPL
                     PVSPDTVTDMDGF"
     gene            6064..9174
                     /locus_tag="PKM27_10275"
     CDS             6064..9174
                     /locus_tag="PKM27_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNR07688.1"
                     /translation="MGTAFAQINVTGTVKDENGEALPGVAIQVKGTSTGTVTDIDGMY
                     RISAQPQDILIFTYVGMQPHEVNVNGQSQIDVTMTADVEILSEVIVTALGIKEDRKKL
                     SYSAQSIGGQELQETQRDNAVMGLQGRVAGLSLTPTSGIPGSAVNITLRGVNSIGNSN
                     QPLFVVDGLPINSGTFDQHNLYSDAAGVSGNVNNNRDDIANRVTDLNPADIENITVLK
                     GPEAAALYGNEGANGVILITTKKGKAGVGRLNYNNRFTVSSLNLFPETQTAYGRGLNG
                     AADPNTANYFGAKIPSGTPLYDNIGAFYQNGSNMRHDLSFEGGATNLTYRLSGSYFNA
                     NGVIPENNQKQLNVALNLDANLLKNLRGSARINYISNDSKIPPAGALGYLLSVLAYPR
                     TDDITQYLNPDGTRRQTTSLSPASELDNPLFLVNKNKRTDHTNRTLTNISFTYEPLNW
                     FSLTARMGADIYSTLGNRFYHPESVIGNARRGWIENYSDDGRILNGNIFATFKKKIST
                     LNGSLLVGSSVDDRQADINASYGEKFYLPEFNSLNNTDPTTQRDRSNLRRTRLLGAFA
                     KAEISLNDILIFNLTGRNDWSSTLPKSNRSYFYPSAGVTFLLTSLPMFKENDRVLSFA
                     KLRASYAEVGNPAPAYLIQARLAPQPSTGGGFLFDFYGDNPALKPEKVNSYEIGGDFS
                     FFKGRLNADLAWFSKSIEDQIITQRLSYGTGFIFGLLNGGELNTKGWEIQLGGKPFRN
                     NTIDWEINANFTKYSTKVISLPANVNEYYSSDTWVYGNARASAFSPSAILASRFTSAN
                     NRYYAPVNDRGAGTATAIGGYSYLRNSKGDILVDPNTGFPLSNNNFLPIGDRNPDFTV
                     GLVNKLSFRKNLSLSFLVDIRKGGDIFNGNEMYLTQTGLSVNTLDREKPFVVKGVLKD
                     GKEESENPTVNAREISRFSSSLYYTTYVQPEDFVEKDINWLRLRDITLKYDFPSSFLQ
                     RSRVFKSLGIFVNGTDLLLITNYSGADPYVSSTTPATGGAGGFGFDYGKLSLPKTFSC
                     GLTIGL"
     gene            9211..10728
                     /locus_tag="PKM27_10280"
     CDS             9211..10728
                     /locus_tag="PKM27_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNR07689.1"
                     /translation="MKTNSKYKFSLLALFLVFASCEDFLDVNFDPRNPQVAQGFAILP
                     PILSQMARGESFDTRYIGAYTQNWCTRLANNVWDLHGYVAGSDAAGELWRSNYWSIGK
                     NIDLIVDDATAKEQWDYVGAAYGIRAWSWQSTTDYHGEIILKQAWEPNRYVFDYDSQE
                     EVYAEVVRVAEMALENLNKTGGGVAESSLGRGDLVYKGNREKWIKFVNGVLARNANHL
                     SNKASYSPDKVIGFVDKSFASNADNFAIPHAGTNSSDGNFYGPTRNNLANFRPTKFIV
                     SLLDGTVFNGVVDPRIGLMMTASPDGVFRGVTPGKVDSTNVTNDPRRIPTLWGGSPAL
                     AASAQPGKYIFTDKADHLIMTYWEMQFIKAEAAFKKGDKTLAFDAFRKGISAHMDFCK
                     VTTANKEAYLSSAALPQSSDALTLSQIMLQKYISMWVAGTIETWVDMRRYHYDPAIYT
                     GFTLPSPLAAGNNDKPAYRVRPRYNSEYVWNLETLNKLGAADLDYHTKEMWFTKP"
     gene            10789..11883
                     /locus_tag="PKM27_10285"
     CDS             10789..11883
                     /locus_tag="PKM27_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNR07690.1"
                     /translation="MESKSLTIPVLLLVLTIFRFQVTTAQEDSAKALSLSGYMETYYS
                     YDFDHPGTHVRQPFLYNFNRHNEFNLNLGLIKLAYQTTKVRGNLALMAGTYPNDNLSA
                     EPGVMKNVFEANAGIKISGSKNLWLDAGIFSSHLGFESAIGKDCWNLSRSLLAENSPY
                     YLSGLKVTYISGDAKWVLSGLVVNGWQRIYRVDGNNTPAFGHQLSFKPSPAITLNSSS
                     FIGSDKPDQVRQLRFFHNFYGIFQVNDRMAFTTGFDFGAEESAIGNGEYNTWYAPVLL
                     LKWSPGPKWNLAARLEYYSDANGVIIATGAPHGFKTWGYSLNLDFPLYDQVLWRVEGR
                     GFKSKDPLFNRGGPPSKTNASITTSLAISF"
     gene            complement(11932..12954)
                     /locus_tag="PKM27_10290"
     CDS             complement(11932..12954)
                     /locus_tag="PKM27_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR07691.1"
                     /translation="MPLIKPARTVFQIVKTKIFLFFGLLAVGLSGQGDPLVLYNKGAE
                     LAIARDFKGAIEAFDEAIRLDPNLYYAYAGRAAVKAEQGNYKAALADYQAYEKIVTEL
                     NLLGDPEILAKKAKVEELMQAVQEDGKSKNGNGGKPDEEISQAKTMTKLNEAIEANDE
                     PRVYYYRGKLKLETNEITAAIFDFSQSLLLDSGFVDAWVARGYARLKLLDLAPALNDF
                     SRAISLDPTNYEALIGRGEVKDKQKIYLSAIDDFSAAALAVPSNHVAYFDRGLTWFHL
                     KNFEKANDDFSMAIQLNSSHTRAYFNRAITRINLRKRTEACIDLKTASDLGHPQAAEY
                     INRFCN"
     gene            13133..17197
                     /locus_tag="PKM27_10295"
     CDS             13133..17197
                     /locus_tag="PKM27_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR07692.1"
                     /translation="MKYNVRYLLSSWLSMGLACTIAAQTSPGPNQAAPDQPYRIALSS
                     SNQPFAFHKVVINPKGKVYGLTGDRKDQLKALNQDGQSLFSVSGEFEQAAGLVATEQS
                     VYVLDQKEKLVFRFDANTGKLQNRFGDQAEERLRKPSSLGLIQGQLLLFDEGKNNVHF
                     FDLEGKFIKTVLLNGVQSPAEVYTNQRDAFYVLDDKLNAILKFDANVKPSPSGYLRLG
                     TSRPEKIECFTVDPLGYLLIQNGTTKQIEVYDWKEKPMKITQVAGNLETMTVDPSTYR
                     LIGKESNSRQATYEIQVIPPMPENIYGFDIDQNRLLVTFKPLNANLADRYGLLTYASD
                     GSDSLAYMTFGNQFVIDESTIFKGKSRRYKLVSMNPSAMSAPTTGFDNFFGLGNYLNK
                     AGNYEDAMLAYQNAIRYMGRPQKMVAFVAQAMLDMGKAQLASSPELIKGLNMLKSAYN
                     LNPRSPAIQEGLASGYQTLFWKLAAQENYTTILEEAGKVVSQTFLKPFILAGVDSVAG
                     VLENLPNVNTLTNARLMRIKLIEWAPVVPHYHTAAYQTGMRLYELKFRSGAPEYELQA
                     LLGECERNIKKTIDLLIAAKKPFQQEYLQHLEVLKLSNKYADLEKRAKESITVYGARF
                     SSEQTITIREYMAYALNGLKRYDEAIAEYEFLLGQKPNHTGYLTGLAETQVQAGNHPE
                     ALALYKQLLLNDRNNPQIIGKAGMIELLLGNFAEASFQLEKASQMDPSNKSFYGPLGE
                     AFEKTSSNQKALENYKLAIQYYETGKPNLEEAREKLKYYRNKTAALYSRMSDHAHAVD
                     VYKKLANQNPDEAVNWYNLGTSSLSAGLVFDAVNALQTAQGLNPGDTKIRTALQNAID
                     LREKVSANEEPLTIAEVRIPEIYPSLFNNYADLSLLPIGDLIVANNTKLPLKYSAMTL
                     DIPDLMPQPTIQNEGILVGFSNSTLSLRAIFSPAILNQTQSQTLQATVSIQYTYENAP
                     KTTSKTIPVTVHSRNAINWSDKRKLASFISQGSGPVTDFSRKALEYFSPVPSNTLNEN
                     LASALKLYSTLGSMRLSYLPDPELNYTTVSINTNLLDFLQYPSETLIRKTGDCDDLVT
                     LYCALLENAGVSTAYIDVPGHIFMALDLKLTPDEANEQGFQSTDIIVYQGKTWLPVET
                     TLIGSAPFSQAWEKGASRYYQEISKGNFPEIVPLVDARKVYRPSDFAPEALPSLPRDQ
                     QPVFREFTQQMQWIFQKLNATSLTVMKNQYLNEPNNVYIKNKLAVLLAQTGQWDEARR
                     ILEEALKLAPENPSVYNNLGNIAFQQNQFAEAIQFYEKSYTFDDSDIQTLINLTKANL
                     AILDKAAARKWFDRAASINPNIAKHYSLLLNQLQ"
     gene            17194..17913
                     /locus_tag="PKM27_10300"
     CDS             17194..17913
                     /locus_tag="PKM27_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR07693.1"
                     /translation="MIPKITLICFLLVLGSNLVHGQNTANYQVGKITYLEGQATLTAG
                     TQAVNVGINTPVLYLQTIKTAAKSVAEISWTNGSKTTVEPSSTYLIKDLYDQSNSKAL
                     AQSESVFSGFKKVFRAANESRKAEEGGIRRSKVNADTLPKPDQLYWKEDREVSFEEAS
                     AYYEKGDYPKAIWAFRTFLDQKPMDSMAKYATFALGHSYLLANNQVKAREIFEKFILK
                     YSNDELKNQAELVLTKFPASN"
     gene            17910..20135
                     /locus_tag="PKM27_10305"
     CDS             17910..20135
                     /locus_tag="PKM27_10305"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR07694.1"
                     /translation="MKFKKISRFFPRAFILSLISIALTAGVVWLSNNKLVTSPVKQLE
                     NTLYDLGFKGRSVNKAHKLIRPEDIAIVAVDNASIDELGRVQLWPRLYDGLVMEKIAA
                     AGAIAIGVDMLYTETDTLPAIYTRMLSQAGIMDSAKVISALSTDQDLSRAIEKAGNVY
                     LSLFDNDADSSRAVSDSVIRPLRLLTAPVSPERKFSRMIYPQLPLASFTRGARAVGSI
                     NMPSDLDGVVRDYQLVQQVGDEKDGQVRYLANFPYYMLADLLGVPESEVRFEGSTLCL
                     TDSFKIPLNRKGSFKINWLGTNEDIRMISFYKVLNDIVPAEYFKDKVVFIGASASGLE
                     DIKTTPDADDRVPGVKIHAVAFLNMINQAFFTNFNSIWFYLVLLLITFLISIALSNME
                     PMFGLLFLAGAMLLEILVAVVFLFQQWGVMIPLGTLLGSTLLVSILTTTYSYFTEQKQ
                     KRQLKNAFGTYVSATVVNKILEDPKSLMLGGTKKVLSVLFSDIRGFTNYSEKMDPQLV
                     VTILNRYLSAMSEPILSHEGTIDKFIGDAIFAIFGAPLEFSDHADEACEVALEMFHKL
                     DEVNEWIVSNKFEPLKIGIGINTGEMTIGNIGSSKRFDYTAIGDAVNLGSRTEGLTKY
                     FNVSVLVTEYTKDYCKSGQFLFRALPCTKVAGKAETVGMFELVDYKANRAKYEPHISL
                     WEQALQAFHDYKFDESYDLFAQCNQLKKDDYATEIYMKKCKEYKVHPENFSNILEMGS
                     K"
     gene            20296..23082
                     /gene="polA"
                     /locus_tag="PKM27_10310"
     CDS             20296..23082
                     /gene="polA"
                     /locus_tag="PKM27_10310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNR07695.1"
                     /translation="MPQKLFLLDAYALIFRAYYAFIRNPIINSKGVNTSAIFGFTNTL
                     LEILEKQKPSHIAVVFDHPSQTLREQEYNHYKANRDETPQPIKDAGPWIRDIIRGMNI
                     PIYDYEGYEADDVIATLAKKGAAAGHEVYIMTGDKDLAQCVTDQVKIYRPGKQGGPAE
                     VLGIEEVKQKFQIKTPLQVIDLLGLMGDAVDNIPGVNGVGEKTAVKLIEEFGSIETLY
                     ERIDEVKGKIKDKLIEGKENAFLSKRLATVVSDVPVEFNEEELSIGAPNREVLAPIFS
                     ELEFRTIGKRLFGDEFSVTRKTTSPSAPTLFEDVQEEDSPPDHGVSAAYTIRNTAHEY
                     QIAGTPALRAELISVLMKQKEVAFDTETTGLDSLNTRLIGMSFSFEEHKAWFVPVPES
                     FEEARALVRAFQPVLENPSIIKIGHNIKFDWQVIRQYGVEIKSPFYDTMVAHYLIDAN
                     QRHKMDYLAETYLQYSPVPIEALIGKAGKKQKSMSEVPLAELAEYAAEDADVTFRFKK
                     VFDPLLKQKELTRLFEEVELPLVETLQDMEWHGVQVDAAFLGEYSKSLGKELSEIKEK
                     IYQQAGAEFNLDSPKQMGEILFDRMKIPYTGPKTKTGQYQTNEDTLTKLAETHPIIES
                     IVNYRELAKLKSTYVDAIPELINPKTGRLHTTFNQTIAATGRLSSINPNLQNIPIRTD
                     RGKEIRKAFIPRDAEHVILSADYSQIELRIIAAISKDENMLEAFRRGEDIHTATASKV
                     FNVASGEVTKEMRRKAKAVNFGIAYGQSAFGLSQTLNIARSEAKEIIDSYFTQFPGIK
                     QYMQDTISFAKEHGYIQTLLGRRRYLPEIHERNFTVRAQAERNAINSPIQGSAADMIK
                     VAMIRIHQRLKKENFHSVMTLQVHDELVFDALRNEVEGIKPLIEEEMKNAIPNLPVPI
                     VVEMGTGNNWLDAH"
     gene            complement(23262..23726)
                     /locus_tag="PKM27_10315"
     CDS             complement(23262..23726)
                     /locus_tag="PKM27_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07696.1"
                     /translation="MTHSLSLSVFIVLVSACSASRQMTKESAIGVLYYQNYDLKISRA
                     GAMDTAYYVITNAKEFEDVFTRVREVDAKAPNFTGQTLVAISAPAATRLRVIRAAIAS
                     SDMNVYAGTCDANEAGCPENGLAVSTVPKSFSVKRVVFYINSRLVKQLPVSY"
     gene            complement(23870..24352)
                     /locus_tag="PKM27_10320"
     CDS             complement(23870..24352)
                     /locus_tag="PKM27_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNR07697.1"
                     /translation="MNPRIETIGPKKLVGKRLTMSLSGNQTAELWKSFMPRRGDLVNA
                     MNKEVISLSIYPPAYFVNFSPDQEFEKWAAMEVSDFDRVPGDMEAFSLEGGLYAVFEY
                     KGSSRDNRIFQEIFGTWLPGSAYELDQRPHFELLGPKYKNNDPSSEEEIWIPVRPKGT
                     "
     gene            complement(24479..25141)
                     /locus_tag="PKM27_10325"
     CDS             complement(24479..25141)
                     /locus_tag="PKM27_10325"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNR07698.1"
                     /translation="METQSVSLEKKSGTLIVTLLKCGVLSSMLYIAMNVFIPMFHPGY
                     SSFSQTVSELSAVDSPTRTLWMVLISLYSLLVFVFGWAVRSVAQGDRRLRIAGILMMV
                     YVIVGLVWPPMHQREVLAAGGGTLTDTLHIAFTMFTVPLMLLIMGFGSAVFGKRFRFY
                     SLVSILIMIVFGVLTGLQSARMEANLPTPWMGVWERISIAAYMVWIMVFVVMLLKRAR
                     TG"
     gene            complement(25324..26349)
                     /locus_tag="PKM27_10330"
     CDS             complement(25324..26349)
                     /locus_tag="PKM27_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR07699.1"
                     /translation="MKRLIYLLFCPFIFSCVEKSNKARPGPDTASVTVNEQTFLELGG
                     EKQYVEITGTTPDKPVLLFLHGGPGWPQTPHLRYFNAGISDSMTLVAWEQRGCGKSYM
                     QNPAAQNMSLERIVEDGHELTLWLKEKFWKDRIYLAGFSWGSVVGLKLIEKYPEDYAA
                     YFGIAQVLNIRQSIKRSRIWIAEQARGKDDIQTLRVLDQLEKGDTSLCKGELNCFLKQ
                     HELLSKYGGAIFSKEAEKEIQKAETQYEDYKNYDWFAAFKYSAEHLEKDMFEVDLTGI
                     RSLKIPVYFLLGRHDWNLVSAITEEFAKQLNAPHKEIIWFEQSGHEPLEEEAGKFNTT
                     IIHLVGK"
     gene            26570..27496
                     /locus_tag="PKM27_10335"
     CDS             26570..27496
                     /locus_tag="PKM27_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HNR07700.1"
                     /translation="MKFPKVFSAGELAALCEAEVLGDPSTPLQGMNEIHKVQPGDLMF
                     VDNEKYFRKSLESEASAILINQKIPVPAGKVLLYHPQPFDAYNALAWHFRPFMHTREA
                     INPLAMIDPSATIESGVVIGAFARIGAGTYIQANAYIGPYTEIGEKVIIHPACVIGSD
                     AFYYHKTNLVYTKWRSIGRVVVEDEVEIGSNCTIARGVSGDTVLGYGSKLDCQVHIGH
                     GVRVGKHCLMAAQVAIGGKTLIGDHVTMYGQVGVAQNLKIGDHAVLLAKTGVSKDLPG
                     GVTYYGIPAEPANIAYKQLAMMRQLARSKNKS"
     gene            27493..29976
                     /locus_tag="PKM27_10340"
     CDS             27493..29976
                     /locus_tag="PKM27_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07701.1"
                     /translation="MIHPYWISYLIALFFTLEPGERPSDWFYRQRAWPLDSIPQQFYQ
                     KAVGEAMLKARASYREYNQAWTFAGPMNIGGRITDVEGVNGNANIIYVGAASGGVFKT
                     TNGGQAWQAIFDQNPVLSIGDMAIHPSNPNILWVGTGEANGGGGSVSYEGNGIFKTTD
                     GGNSWIYLGLEQSGSIPKILIHPFDDKIIYVAAMGRMFGKNKQRGVFKSINGGDTWEQ
                     VLFHSDSVGAIDLILDPLHPDTLYAALWERSRRPSGIDYGGPACGIYRSFDGGKVWTP
                     LSQGLPAGANLGRIGIALCASSPQVVYAYYMDESGDFLGIYRSDNHGNTWTKTNGRVN
                     VANFGWWFGKIYVSPENPDVVYALGLSGTKSVNGGQQFLPMTENFNEDVHVDQHSLFI
                     DEKNPNRLLLGNDGGLYLSQNAGNQWSKINNLPITQFYTCHIDYSQPQRIYGGTQDNS
                     SMRRLPGSQDQWEIIWEGDGFVCLVDPSDNRYVYTESQYGVFVRSTDGGKNFSTALNG
                     IDGNDRKNWNTPVVFNPKNPASLYLGTNRLYKSTDRALSWQVISPNLGGISSSRNYGT
                     ITTISVSPLDSQLIYCGTDNGQAWVTKNGGQSWDQISTGLPQRWITSVAADHNIKSKV
                     YISLSGYRYDESMPHIFRSSDYGTTWTDISRGLPPVPCNQVLVDPALSGHLVVATDAG
                     VWRSYNDGNQWEMPGRDLPLTVYTSLAFHAPTRTLAAASYGRGIYTYTLELPVSQEET
                     SPDPLVFEAFPNPVKEVLTLKFSSPKTQKLTLGLTDLGGRFLWNKTIQVRPGLEYHEK
                     IQWNEGPGIYFLRLQENNGETKTLKILHP"
     gene            30126..31574
                     /locus_tag="PKM27_10345"
     CDS             30126..31574
                     /locus_tag="PKM27_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR07702.1"
                     /translation="MKTTLSPIALLFLFVHLSAQSPLTLADYIRTAQSRSLKSKLAQT
                     RTEISRYQYQSFLSDLRPQISFYGSLPAYSRDYFDVRQPDGTIKFLPRSQNFSNIGFS
                     LSQQILATGGILSLRTDLSRFDDFNLKTKSYNSTPLYLRFDQPLFGFNELKWNKKIEP
                     LKLEESGKSELLELQKIAVDICRSYFEVAEAQLRGQMAEANRTNILELLELEEKRIPL
                     GTTSEEKILQLRLQLIQSRQQADQARYQVQVALLRLNAEAGLQNGETAALMLPEELPV
                     VKLSADTAVYYARKLRPEFLAFQRSSLEAEREVKRADAARKSVNLSASFGYNNAADEF
                     GDLLNGALNQQRLSVGIDLPLVDWGRRDARVQAAKALQRLTQFNNELNEQNYTQEIIT
                     SVSTLELLHSNIQVARETESIARERFEVSLRQYQTGRLGLTELNIARQEKDEARVQHI
                     QALRNWWSGYYELRQLTLYDFEAEREIQILDK"
BASE COUNT         8168 a         8442 c         7601 g         7412 t
ORIGIN      
        1 cataattact aaaatttttt tgtaactatg tctgaaaatt tagggggctg tttcaataga
       61 tttacctgaa aaaaggatga aaagtgatca aatgaccagg ccggatggcc tggagcttat
      121 ttccgcctgg aaatcaagcg aaaagtccag caccgaattt tgcgaggaac gagacatgac
      181 gatataactc atcaccgatt gcaatactgg catgaggtgt ataaaaaggt taacagacag
      241 gaattgggcc ggaaggctga aaagggattt attccgacag ttgtgggttc tacaaccaag
      301 gacgggaatg ataaattgca tctttatatg ccctcttatt aataaatttt aaaaaaaatg
      361 tcacttaaaa ctctcatttt aagtaactta tgatcaaaat cgtggtggtg cctccaggaa
      421 ttgtcccgcg acctcgggac aaggacacca ggatattcta atgtctggat aaaatagaaa
      481 gcctgagtgc ctcatcatgg ataaaacgaa taattaaatt aaatccttgc ttttttgcct
      541 tttaaggaag gcttccattt ttcttgctct gatcctggaa aaaaggttgg cggacctggc
      601 aatccaccaa tcagaagcga tcatagtaaa ggaggattta aacgaatgag accgatgttg
      661 ggctagacgc tcctcaagat tctgagtttc cacgatataa tatttatcca gggacggact
      721 gtaaaggatg taaacaaaat gcatcatgtg ttcaaataaa caaaaagagg ttgccaaacg
      781 acaacctcct ttacttcggt ggtgcctcca ggaattgaac cagggacacc aggattttca
      841 gtcctgtgct ctaccaactg agctaaggca ccatcgccca taaacaagga acttgtttat
      901 aaggcggggc aaatatactt tttttatttt atccaggaaa tccctcactt ctctcggtga
      961 ataccgggcg gacctgtctg ccgccaggca ggcttccaag gacttgttta tttgaggatg
     1021 tataaagctc ccgcaggcgc gggattgcca tgctcctata catggaattt tgttgaagga
     1081 aaccaaccct ttttatgatc acttcggatc caccgcgcga taggccgcca atacggccac
     1141 ttcaccttct ttcccgggtt tgcttgaccc gtgttccagg ccgacaaaac cgcggtatcc
     1201 tttgtcgtaa acatgttgca gaacttttgg gtaattgatc tctccggtcg tgggctcttt
     1261 gcgtcccggg gtgtccccga tctgaatata ggcaatttcg tcccaggcat aatccagggt
     1321 aggcaggaga tttccctcct gaatctgcac atgataaaaa tcaaatagca ccttcacgct
     1381 tgggctccca accgctttag ccagggcata ggcctggggt atggtatgca ggaacatgcc
     1441 cgggtgatcg atcttatgat tcatggattc catcaccatg atgagcccgt gaggttcgta
     1501 tacgccggcg attcgtttta acagctcgac tgcatttgcc atctgaaaat cccagggaag
     1561 ttttgggtcc gccatgccta atacattgtg aataaaccgc gtattcatcc ttttggcaat
     1621 gtccaccgat gcccggacat cctccatgac cttctctctt aagccgccgt cacggcccgc
     1681 aaaggtgacg tccttaaacg tcaaagtacc tacgaactgc ccccactcca tgcccagtcc
     1741 ctgaacggtt ttgctgatcc tttcctgctc ttcgacgggc ctgttcttca gcatggtatt
     1801 ttcccaggcg cgaaatccct gatcatgtcc ccatttgatc tggtccacgg gattgtcgcc
     1861 cgccaggtct ccgaagagcc caaaatccgg ggaaaatttc attttaaaaa caggatcggc
     1921 aataccagga atggcgttgc tgtggaatga aggcaaaata cctgaagcaa gtattccgct
     1981 ctttaaaaag ttccgtcgct tcatataaaa catttattga atatggcaaa tcccgggctt
     2041 cggtttagcg gagcagtctg gcgtcggttt cggggtcctg gtcctgtcct atcagaaagg
     2101 ccaggaggtc cgccatttcc tgttccgtga gcaacttatc cagtcccgca ggcatcatgg
     2161 aatgttccag aatctccgtg gacagaatat cgctgcgctg aatctgcacc gattcctggg
     2221 gagaaatgcc cagccggatc atgtccccgg tttgttcttg aatgattccg gtaaaggtgc
     2281 cttccttggt gctgatccgg taggtttcgt attccctgac aaaactcacc cccgggtata
     2341 cgatcgcctc aaccaaatcg tgagcagacc ggtcgcgctg aatggaagta aggtccgggc
     2401 caaagccagc cccttcattt ccgatgacgt gacaggtata acaaatggct ttgccaagga
     2461 acagttccct tccgcgaaca atgtctcctt ttacaatgga attctcgatg gcatggatga
     2521 gtgacaggcg ttcaccctgt accgccgata atttggccat caattggtcc tttgcttcgt
     2581 tcaattccgg gggatagttt gaaaacaggc catggaggta ctcttccgtg aaattgtcaa
     2641 gaccgggcga acgctccagc gctcctgcaa gggcggttcc tatttctaaa tttttcgcac
     2701 cctgaaagac gggcatcagc ctcggcagga taaaggcatc cgcctttccg agaaattctt
     2761 tggcaatttt aaggagttgg tcatccgaca gtttggcttg tcccaggacc cccgcggcct
     2821 gctgacgtat gctggcttca atatccggct gaatctgggt atagaggtaa tcgaaatgcg
     2881 gactggtaag cagggcgctg tccctcaagg aggtcccgat cgcatggatt ctgagtatgg
     2941 caggattttg cttattcccg gccacttgtt taagggtatg gtccaatgaa gagaccccgt
     3001 acagccggat taactttaat accttaagtt ttacttcgac ctccgcattg gaggtgagtt
     3061 cctttccaag agggccgatc caggcggcag ggaattcttt aatattacag ccgctcatgg
     3121 actcaaggac aaagagcttt ttttcaattt gcggcgctgc catctgatca gccatgaatc
     3181 gctgcatggt gccatccccg caaaaggaaa ccagaatatc tttcaattga gttttgtcgg
     3241 cctcgctcca ctcgggtttg ttcagttgtt tttgcaggta tacaatgaag tcgggcgccc
     3301 attcgggatg gtgactgatc acccaacggg ccgcgctttc cagcatgctg tccgcgctgg
     3361 ctaaaaaagg agtcacctgc tccagtttta atgattggtt ctgcatttgg tccagtgcga
     3421 tcaaagcagc cctcttgacc tcgtccgaag gatgggtgag acccgcctta accaggtccg
     3481 gccggttcat cgagagcagt gaatagatga tggcatgttc aacaaaccgg tccttcactc
     3541 catcggcgga ctgcagcaat gcgggaatcg cacgcggttc gccgatctgc ccaatcgccg
     3601 ttgccgcctg gcgccggact gccggcagat cggtttgaat cgcttgaatg agttgatcca
     3661 ccgaaccctg gtctttcgcc aggcccaggg cttttgccgc ggctacccgc acttgcagat
     3721 ccttatcctt tagtccttcc cttaatgcgg cgatggattc actggaatgg atcctgtaaa
     3781 gggcatacac gatgcgggtg cgggcatggg cgtccgggga attggtcaac aatgcggcca
     3841 gcgaggccac cgactcctga ctgcgatcaa ccagttgatt tacggcccgg tccgccacaa
     3901 aaggccgggc gtcttcaata tacctggcaa tcgtcgcaac atcgaggccg gcccattcaa
     3961 tatggtttcc aaaaggatct tccattttgg cggcgtcttt ttttctcacg cgatagatcg
     4021 atccctttaa ttccggtttc gatacctgtg aaaggggaca gcctttgata aaccatccgc
     4081 cggtttccac gaccagcagg ctaccgtccc cgtcttccaa tacgtcggtc ggatgaaagt
     4141 cttcgttttc ggaagaaaag aaaacttcgt cctccgtccg gaaggaggcc ccttcccgga
     4201 tcaattgatg acgaagaacc cggtgtgtat taaactgggc gctgaataaa ttatcccggt
     4261 agctttcatc caggacgctg ccacggtaac gaatgatccc ggcaggggcc accctggaat
     4321 atttggtcac cacgggcaac aaatccccgg tcaaaggcag cctgtcgcgc gtgatgttgt
     4381 tgttgggctt gggatagacc ccgccttctg tccaatacat cagggcatcc cggaggccgg
     4441 ccttaggctc ggtgaaatag gtttgagtcc ccaacacttc cgatgcggcc gtaaaagcaa
     4501 gtccgaccgg attgttcatg ccaccggcgg atatccactg aagccctgaa ccatccggct
     4561 ttaatctcca gacccttgcc gtttcccctt taaggtgctc gccttcgcgg gtcatgatgt
     4621 caaaaccttc aatggcgctg gtcagataaa gccagccgtc gggcgccata aagggaccga
     4681 tgaggctgtt ggcattgacg ttcaactccc atccggacag cagcacttcc cgttggtccg
     4741 caacgccatc tccgtcggtg tccgtaagtt ttagcaattc aggtgcggaa gaggcgtaaa
     4801 gactaccttt ataaaacacc ccgccttgcg gaaaaccgac tttatctgca aagaccgtat
     4861 acttatcata gaagccgtcc ccatcttcat ccaccaggag ttttatccgg aattgaggat
     4921 tctttaaagc atcttcggtt ttgttgtata cattgccggt ggactcatac acaaaaaggc
     4981 gcccggtttc atcaacaaaa gcaaacatgg ggtagtccac caggtccggc cctgcggcca
     5041 cttcgatggt aaatccctcc ggaacttcaa tgtcacgggt tgctgcatca ggacttttgc
     5101 cgcattggga caaggttaat aaagcactgg ccaaaagcag aaacagacag gaataaagat
     5161 tcttgtggtg gaagttcatg ctgcagggtt gatcattccg taaaataaga ataaatcctg
     5221 agcctggata tggatgtcct ggacaggcct gaagaactaa agtccttcgg agaaaacatt
     5281 tcaaaaatga aaacagcagg ggatcgctat accacaatcc aggcgcaaca gcctgatgaa
     5341 tagcaagtcg gtttaaaccc aaatctttgg acgttgtcaa cccaagcatt cacgccaatc
     5401 ctgcccttct tatggattcc cggtgaaatc cttaattcgt tttccgacac ataacccata
     5461 aaaattgcat gccttccctt gacgtgttca tgtttggtct tttccctgta accggccttt
     5521 ctgcccagaa ttaattgttt aaatttatct ggattaagta taattttagc cggctgccca
     5581 tcgttattct tagtaattcc ttaacgcttt ttgatatgaa attgaaaatc aggttcttcc
     5641 aactacacta tgggatctgt ttcattttct gtgctttttg catgcacctt tcgccgggca
     5701 ccaggtcttt acccggtgag tcgatgatca aagccgaatg gccccttccg gtctccccgg
     5761 atacggtcac ggatatggat gggttttgag gtttaacagt ttagcagttt atcagtttag
     5821 cagttttcca ataaccgtat taggaatggt tggggaaggc agatttcata tgattttaat
     5881 caggtcatcc ggaaaataga atttttttag ctttaagtgg tctaaaacca ccagtttata
     5941 aagataagct gggaaaaaca ccttttcaaa accagattaa accttatgaa aatgctaagt
     6001 attagccgta acaggatagg gatccaccaa tgcctttggg tactcgtcct ttcattccta
     6061 ttcatgggca ccgcgttcgc ccagatcaat gtgaccggga cggtgaagga tgaaaatggg
     6121 gaagccctcc ccggggtagc catccaggta aagggaacct ctaccggtac ggtcacggat
     6181 atcgatggga tgtaccggat ttccgcccag ccgcaggaca ttctcatctt cacctatgta
     6241 ggcatgcagc cccatgaggt caatgtcaac gggcaaagcc agatcgacgt gaccatgacc
     6301 gcggatgtgg aaattctttc agaagtgatc gtcacggccc tcggtataaa agaagaccgc
     6361 aaaaaactga gttattctgc ccagagcatc ggtggacagg aattgcagga aacccagcgc
     6421 gacaatgccg taatgggttt gcaaggtcgg gtagccggcc tttcgctcac gcccaccagc
     6481 ggcatcccgg gatcggcagt aaacattacc ctgagggggg tcaattccat cggcaacagc
     6541 aatcagcctt tgtttgtggt ggatggcctg cctatcaaca gcggcacctt tgaccagcac
     6601 aatctttatt ccgatgcggc gggggtcagc ggcaatgtca acaacaaccg tgacgacata
     6661 gccaaccggg tgaccgatct caacccggcc gatatcgaaa acatcacggt tttgaaagga
     6721 ccggaggcag ccgccctgta tggaaatgaa ggcgccaacg gagtcatcct catcaccacc
     6781 aaaaaaggca aggccggcgt cgggagatta aattataaca accgcttcac cgtatccagc
     6841 ctcaacctct ttcctgaaac ccaaaccgcc tacggcagag ggctcaatgg cgccgcggac
     6901 cccaataccg ccaattattt tggagccaaa atcccttccg gcactccttt atatgataat
     6961 atcggggctt tttatcaaaa cgggtccaat atgcgccatg acctcagttt tgaaggcggg
     7021 gccaccaacc tgacttaccg tctttcgggc tcctatttta acgccaatgg ggtcatccct
     7081 gaaaacaacc agaagcaact caatgtcgcc ctgaacctgg atgccaacct gttgaaaaac
     7141 ctgagaggct ctgcccggat caactacatc agcaatgatt ccaaaatccc gccggcagga
     7201 gccctgggtt atcttctgag cgttttggct tatccccgga cggacgatat cacccaatac
     7261 ctcaatccgg acggcacccg caggcaaacc acttcgctga gcccggcatc ggaactcgac
     7321 aatccgctct ttctggtcaa taagaataaa aggaccgacc ataccaaccg gaccctcacc
     7381 aatatttcct ttacttatga acccctgaac tggttttctc tcacggcccg catgggagcc
     7441 gatatttaca gcaccctcgg caaccgcttt tatcacccgg aatccgtgat cggcaacgcc
     7501 cggaggggct ggattgaaaa ttattccgat gacggtcgca tcctcaacgg caatattttc
     7561 gccaccttca aaaagaaaat ttccacgctc aacgggtctt tgctggtcgg atcttccgtg
     7621 gatgaccgcc aggcggacat caatgcttct tacggcgaaa agttttacct tcctgaattc
     7681 aacagcctca acaataccga ccccaccacc cagcgggacc gcagcaatct ccgccggacc
     7741 cgcctcctgg gcgcttttgc aaaagcggag atcagtctca acgatatcct catctttaac
     7801 ctgaccggaa ggaacgactg gtcgtccacc ctcccaaaat caaaccgctc gtacttctat
     7861 ccttctgcgg gggtcacttt tttgctgacc agcctgccca tgtttaaaga aaacgaccgg
     7921 gtcttgtctt ttgccaaact cagggcctcc tatgctgaag taggaaatcc cgcaccggct
     7981 tatctgatcc aggcaaggct tgccccccaa ccctctacgg gcggcggatt cctgtttgat
     8041 ttttacggag acaacccggc cctcaaaccg gaaaaggtaa acagttatga aatcgggggc
     8101 gacttttcct tcttcaaagg acgtctcaat gccgacctgg cctggttttc aaaaagcata
     8161 gaggaccaga tcatcaccca gcgtctgagc tacggcaccg gttttatatt cggtttgctc
     8221 aacggaggag aattgaatac caagggatgg gaaatccagc tgggaggcaa acctttccga
     8281 aacaatacca tagactggga gatcaacgcc aatttcacaa aatactccac caaagtcatc
     8341 agcctgccgg ccaacgtaaa cgaatattac agttcggata cctgggtcta cggaaatgcc
     8401 agggcgagcg cgttttcacc cagtgccata ctcgcctcca ggtttacctc cgcgaacaac
     8461 cgttattatg cccccgtcaa cgatcgcgga gcgggcacag ctacggccat cggaggatac
     8521 agctacctga ggaacagcaa aggggacatc ctggtggatc ccaatacggg cttcccgctt
     8581 tccaataaca attttttacc gatcggggac cgcaaccccg atttcaccgt agggctggtc
     8641 aacaagttga gcttccgtaa aaacctgagc ctgtccttcc tggttgatat tcgcaaaggc
     8701 ggggatattt ttaacggcaa tgaaatgtat cttacccaaa ccggcctgag tgtgaatacc
     8761 cttgaccggg aaaaaccctt tgtagtcaaa ggcgtgctca aagacggtaa ggaagaatca
     8821 gaaaatccca cggtcaatgc ccgcgagatc agccgtttta gctcctccct gtattatacc
     8881 acctatgttc aaccggaaga tttcgtggag aaggacatca attggctgcg gctccgcgac
     8941 attaccctta agtatgattt cccttcctcc ttcctgcagc gttccagggt gtttaaatcc
     9001 ctggggatat ttgtcaatgg caccgattta ttgctgatca ccaattattc gggggccgat
     9061 ccttatgtca gcagcaccac ccctgccaca ggaggcgcgg gggggttcgg atttgattac
     9121 ggaaagcttt ccttgcccaa aactttttcc tgtggcctga ctatcggcct gtaatcgcat
     9181 ttcattcatt gaaaatcaat tctgaaagtc atgaaaacaa attccaaata taaattcagt
     9241 ctactcgccc tcttcctggt ctttgcttcc tgcgaagact ttctcgatgt caactttgat
     9301 ccccgcaatc cccaggttgc ccagggtttt gccattctgc cccccatcct gtcccagatg
     9361 gccaggggag aatcctttga tacccgttat atcggagcct atacccaaaa ttggtgtacc
     9421 cggctggcca ataacgtctg ggaccttcac ggttatgtag ccggcagtga cgctgccggg
     9481 gagttgtggc gctccaatta ctggtccata ggtaaaaaca tagacctgat cgtcgatgac
     9541 gctacggcta aagaacaatg ggactatgtg ggcgccgctt acggcatccg ggcctggagc
     9601 tggcaatcca ccaccgatta tcacggagag atcatcctca aacaagcctg ggaacccaac
     9661 cgctacgttt ttgattacga tagccaggag gaggtctatg cggaagtggt acgtgtggct
     9721 gaaatggcat tggaaaacct gaacaagacc ggggggggcg tggccgaaag ttccctgggc
     9781 aggggcgacc tggtctacaa aggcaaccgt gaaaagtgga taaaattcgt gaacggagtg
     9841 ctggcccgca atgccaatca tctctccaac aaagcctcgt attccccgga caaggtcatc
     9901 ggattcgtgg ataaatcttt cgcatccaat gctgacaatt ttgccatccc ccatgcgggc
     9961 accaattcct ccgatggaaa tttttatggc cccacgagaa acaacctggc caatttcagg
    10021 ccgaccaaat ttatcgtcag cctgctggac ggcaccgttt ttaatggcgt cgtggatccc
    10081 aggatcggcc tgatgatgac tgcaagcccg gacggtgttt tcaggggcgt gactccgggt
    10141 aaggtcgatt ccaccaatgt caccaacgat ccccgccgca tccccaccct ctggggcggt
    10201 tctcccgccc ttgcggcctc tgcccagccc gggaagtata ttttcacgga caaagcggac
    10261 cacctgatca tgacctattg ggagatgcaa ttcatcaaag ccgaagccgc ctttaaaaaa
    10321 ggggataaaa cccttgcctt cgatgccttc cgaaaaggca tctcggcgca tatggatttt
    10381 tgcaaggtga ccacggcaaa taaagaagct tacctgagca gtgcggccct gcctcagagt
    10441 tccgacgcgc ttaccctctc ccagatcatg cttcagaaat acatttccat gtgggtggca
    10501 gggacgatcg aaacctgggt ggatatgcgg cgctaccatt acgatccggc catttatacg
    10561 ggctttaccc tgcccagccc tctcgcggcc ggcaacaacg acaaaccggc ttaccgggta
    10621 aggccgaggt acaattccga gtatgtgtgg aatctggaaa ccctgaataa actcggggcg
    10681 gccgacctgg attaccacac gaaggaaatg tggtttacca agccttgaac agcccggtaa
    10741 ggaagttatc aattattgtc cggtaaataa gggaatggcc ttcggaatat ggaaagcaaa
    10801 tcgctcacca tacccgttct gttgcttgtc ttaaccatat tccgtttcca ggtcaccacg
    10861 gcgcaggaag acagcgcaaa agcccttagc ctctcagggt atatggaaac gtattacagt
    10921 tatgatttcg accatcccgg aacgcatgtc cggcagcctt ttctgtacaa tttcaaccgg
    10981 cacaatgaat tcaaccttaa tctgggcctg atcaaactgg cataccaaac cacaaaggtc
    11041 agaggcaacc ttgccctgat ggcgggcacc taccccaacg acaatctttc tgccgaaccc
    11101 ggggtaatga aaaacgtctt tgaagccaat gccggaataa aaatctccgg gagtaaaaac
    11161 ctgtggctgg atgcgggtat tttttcctca cacctcggct tcgaaagcgc catcgggaaa
    11221 gattgctgga atctgagccg gagccttttg gccgaaaaca gtccctatta tctgagcgga
    11281 ttgaaggtta cttacatttc cggcgacgca aaatgggttc tgagtggcct ggtggtaaac
    11341 ggctggcagc gcatttacag ggtggatggg aacaataccc ccgctttcgg tcatcagctt
    11401 tcgttcaaac ccagcccggc aatcactctc aacagcagtt cctttattgg gagcgacaaa
    11461 ccggatcagg taagacagct gcgttttttt cacaattttt acggaatctt ccaggtgaat
    11521 gaccgcatgg ccttcactac cggttttgac tttggagcag aagaaagcgc catcggaaac
    11581 ggagaataca acacctggta tgcacccgtt ctcctcctga agtggagccc tggccccaag
    11641 tggaacctgg ccgccaggct tgaatattac agcgatgcga atggagtcat tattgctacc
    11701 ggcgccccac atgggtttaa aacctgggga tattcactca atcttgattt ccccctctat
    11761 gaccaggtcc tctggcgggt ggaaggcagg ggtttcaaaa gcaaagatcc tttatttaat
    11821 cgcggcgggc caccctcgaa gaccaatgcc agcatcacta catctctggc catatccttt
    11881 tagatccgga aagtttcccg caaggaccac atcccagatg atgcaattca tttaattgca
    11941 gaaccggttt atatactctg ccgcctgcgg gtgccccaga tcactggcgg ttttgaggtc
    12001 gatgcaggcc tctgtccttt ttctgaggtt gatacgggta atggcgcggt tgaagtaggc
    12061 gcgcgtatgc gatgagttca gttggatggc catgctgaaa tcatcattgg ccttttcaaa
    12121 attcttaaga tggaaccagg tcaggccacg gtcgaaataa gccacgtggt tggagggaac
    12181 cgccaaagct gccgcggaaa aatcatcgat ggccgagagg tagatttttt gtttgtcttt
    12241 gacctccccc ctgccgatga gggcttcgta attggtgggg tccagggaga tcgccctgct
    12301 gaagtcattg agggccgggg ccaggtctag caatttgagc cgggcataac cccgcgccac
    12361 ccaggcatcc acaaaaccgg aatccagcag cagggactgg ctgaaatcaa agatggcagc
    12421 ggtgatctca ttggtttcca atttgagttt gccccggtaa taataaaccc tgggctcgtc
    12481 gttggcttca atcgcctcat tgagcttggt catggttttg gcctggctga tctcttcgtc
    12541 cggtttccct ccgtttccgt tcttggactt gccatcttcc tgcacggcct gcatcaattc
    12601 ttcgaccttt gcctttttcg cgaggatttc tggatcaccg aggaggttca gttccgtgac
    12661 aattttttca taagcctggt aatcggcgag ggctgctttg taatttccct gctccgcctt
    12721 cactgcggcg cggccggcat atgcataata aaggttgggg tccagcctga tcgcttcatc
    12781 aaaggcctca atggcgcctt taaagtcccg ggcgatcgcc agttccgcac ctttgttata
    12841 caggaccaga gggtccccct gcccgctaag gccaaccgcc aataatccaa aaaacaggaa
    12901 aatttttgtt ttcacgatct ggaatacggt tctggccggt tttataaggg gcataatctt
    12961 gctggactga tttaattgaa tcattaaaat tactcaaaat tacagccctt tcccgttttt
    13021 aaaaaaacgg taactaaagg catcccccct tctccgtttc taaaaccggg ttccccaata
    13081 ttgtgcaatc gttttcgaca taagtcgcta actttgaagg ctcaattacc caatgaaata
    13141 taatgtgagg tacttgcttt cgtcatggtt gtccatgggc ctggcttgca cgattgcggc
    13201 ccagaccagc ccgggaccaa accaggccgc cccggaccag ccttaccgga tcgccttatc
    13261 ctcttccaac cagccttttg cttttcataa ggtggtgatc aaccccaaag ggaaagtcta
    13321 tggccttacc ggggaccgga aagatcagct caaagccctg aatcaggatg gacagtccct
    13381 gttttccgtt tcgggggaat ttgaacaggc cgccgggctg gtagccacgg aacaatccgt
    13441 ttatgtcctg gatcaaaagg aaaaactggt tttccgtttt gatgccaata ccggcaaact
    13501 gcaaaaccgc ttcggggatc aggccgagga aaggttgaga aaaccaagtt ccctgggttt
    13561 gatacaaggg caacttttgc tttttgacga agggaaaaac aacgttcatt ttttcgacct
    13621 cgaagggaag tttataaaaa ccgtcctact caacggggta caatccccgg cagaggtgta
    13681 taccaaccaa agggatgcct tttatgtcct ggatgacaaa ctgaacgcca tcctcaaatt
    13741 tgatgcaaac gtaaagcctt ccccatccgg ataccttcgc ctagggacca gcaggcctga
    13801 aaagatcgag tgttttacgg ttgacccgtt gggttacctt ttgattcaaa acgggacgac
    13861 caaacagatc gaagtatacg attggaaaga aaaaccaatg aaaatcaccc aggtggcggg
    13921 caacctggaa accatgacgg tggacccgtc cacttaccgg ctgattggaa aggaatccaa
    13981 ttccaggcaa gccacttacg aaatccaggt catccctccg atgcctgaaa acatttatgg
    14041 atttgacatt gaccaaaacc ggcttttggt gactttcaaa cccctcaacg ccaacttggc
    14101 tgaccgttat ggattgctga cctatgcctc cgacggatcc gactccctgg cttatatgac
    14161 tttcgggaat caatttgtca tcgatgaatc cacgattttc aagggaaaaa gccgcaggta
    14221 taagctggtc agtatgaacc cttcggccat gagcgcgcct accaccggtt ttgacaattt
    14281 tttcgggctg ggcaattatc tgaacaaagc ggggaattac gaagacgcca tgttggccta
    14341 tcaaaacgcc atccggtaca tgggccggcc ccagaaaatg gttgcctttg tggcccaggc
    14401 catgctggat atggggaaag cccaattggc cagcagccct gaactgatca agggactcaa
    14461 catgctgaag tcggcctata acctaaatcc gagaagtccg gcgatccagg aaggactggc
    14521 ttcgggttat cagaccctgt tctggaaact ggcggctcag gaaaattata caaccattct
    14581 tgaagaagcg ggaaaggtgg tcagccagac ctttctcaaa ccctttatcc tggccggcgt
    14641 ggattcggtg gcgggcgtgc tggaaaacct gcccaatgtc aataccctga ccaatgcccg
    14701 gctcatgcgc atcaaactga tcgaatgggc cccggttgta ccgcattatc acaccgccgc
    14761 ttaccagacg ggcatgcgcc tctacgaact caaattcaga tccggcgccc ccgaatatga
    14821 attgcaggcc ttgctgggcg aatgtgaaag aaatatcaaa aaaaccattg acctgcttat
    14881 tgcggctaaa aaaccattcc agcaggaata cctccagcat ctcgaggtac tgaagttgag
    14941 caataagtat gcggatctgg agaaaagggc aaaggaatcg atcaccgttt atggggcccg
    15001 gttcagcagc gaacaaacca taacgatccg ggaatatatg gcttatgccc tcaacggctt
    15061 aaaaaggtat gacgaagcga tcgcggaata cgagtttttg ctcggccaaa aacccaatca
    15121 taccgggtac ctgaccggcc tggcagaaac ccaagttcag gcaggaaacc acccggaagc
    15181 cctggccctc tacaaacaat tgttgctgaa tgaccgcaac aatcctcaga ttatcggcaa
    15241 ggcgggcatg atcgaactct tgttgggaaa ttttgcagaa gcttcgtttc agctggaaaa
    15301 agcgagccag atggaccctt caaacaaatc cttttacggg cccctgggcg aagcgtttga
    15361 gaaaacctcc agcaaccaaa aagcccttga aaattataaa ctggccattc agtattatga
    15421 aaccggaaaa ccaaacctgg aagaagcccg ggaaaaactc aaatattacc ggaacaagac
    15481 cgctgcctta tattcaagga tgagcgatca cgcccatgcc gtggacgtgt ataaaaaact
    15541 ggccaatcag aatccggatg aagccgtcaa ctggtataat ctcggcacct ccagcctttc
    15601 cgccggcctg gtttttgatg ccgtgaatgc gctgcaaacc gcacagggac tcaatcccgg
    15661 ggatacgaaa atcaggaccg cgcttcaaaa tgccatcgac ctgcgcgaaa aggtgtctgc
    15721 caatgaagaa ccgctgacta ttgccgaggt caggatccct gaaatctacc cgtccctctt
    15781 caacaattat gcggacctga gcctgcttcc tattggggac ctcatcgtgg ccaacaatac
    15841 caaactgcct ctgaaataca gtgccatgac tttggatatt ccggacctga tgcctcaacc
    15901 cactattcaa aatgaaggca tcctggtggg cttctcaaat tccacgctca gcctccgggc
    15961 cattttctct ccggccatcc tcaaccagac ccaaagccag accctgcagg ccaccgtgtc
    16021 gatccaatac acatatgaaa atgcccccaa aaccacttca aagactatcc cggtaaccgt
    16081 tcattccagg aatgccatca actggtcgga caaacggaaa ctggcctcct tcatttccca
    16141 gggatccggg ccggtgaccg atttcagccg caaagcgctt gaatacttca gcccggtacc
    16201 ctccaatacc ctgaatgaaa acctggcttc cgccttaaag ctttattcca ctctgggcag
    16261 catgagattg tcctacttac cggaccccga attgaattac acaacggttt cgatcaacac
    16321 caacctcctg gattttcttc agtatccgtc cgaaaccctt atccgcaaga ccggggactg
    16381 cgacgacctg gtcacccttt attgcgccct gcttgaaaat gccggggtat ccactgccta
    16441 tatcgacgta ccgggccaca tttttatggc cctggacctg aaactgaccc cggatgaggc
    16501 caacgaacaa ggctttcagt ccaccgacat cattgtttac cagggaaaaa cctggctgcc
    16561 ggtagaaacc acgctgatcg gaagcgcccc cttctcccag gcctgggaaa aaggcgcatc
    16621 gcgctactac caggaaattt caaaaggcaa tttcccggag atcgtcccgc tggtggacgc
    16681 ccgtaaagtt taccggcctt ctgattttgc cccggaggcc ctcccttcgc tgccccgcga
    16741 tcagcagccg gtgttcaggg aatttaccca gcagatgcag tggattttcc aaaaactcaa
    16801 tgccaccagc ctgacggtca tgaaaaatca atacctcaac gagcccaaca atgtctatat
    16861 caaaaacaaa ctcgcggtat tgctggccca gaccggtcag tgggatgaag cccggcggat
    16921 cctggaggaa gccctgaagc tggccccgga aaacccatcc gtctacaaca accttggaaa
    16981 tatcgccttc cagcaaaatc agtttgcaga ggccatccaa ttctatgaaa aaagctatac
    17041 ttttgatgat tcggatattc agacgctgat caatctgacc aaggcgaacc tggccatcct
    17101 ggacaaagct gcagccagga agtggttcga tcgggcagcc tcaatcaatc caaacattgc
    17161 caaacattat tctttactac taaatcaact ccaatgatcc ctaaaatcac actgatctgc
    17221 ttcctcctgg tcctgggcag caatctggta catggccaga ataccgccaa ttaccaggtg
    17281 ggaaaaatca cctatctcga aggccaggcc accctgactg ccggcaccca agcggtcaat
    17341 gttggcatca atacccccgt cctttacctg caaactatta aaacggcggc taaatcggtg
    17401 gccgaaatca gttggaccaa cggatctaaa actacggtgg agccttccag cacttatctc
    17461 atcaaagact tgtacgatca aagcaattcc aaggcgctgg cccagtctga atccgtgttt
    17521 tctggcttca aaaaggtctt ccgggctgcc aacgaatccc gcaaggccga agagggaggg
    17581 atccgcaggt ccaaggtcaa tgccgatacc ttacctaaac ccgaccagct ctattggaaa
    17641 gaagaccggg aagtcagctt cgaagaagct tccgcctatt acgaaaaagg agattatccc
    17701 aaagccatct gggctttccg gaccttcctg gaccagaaac cgatggattc catggccaaa
    17761 tacgccacct tcgctctcgg ccattcttac ctcctggcca acaaccaggt gaaagcgcgg
    17821 gaaatttttg aaaaattcat cctgaaatat tccaatgacg agcttaagaa ccaggctgaa
    17881 ttggtattga cgaaatttcc ggccagcaat tgaaatttaa aaagatctcc cgcttctttc
    17941 cgcgggcctt tattctttcc ctgataagca tagcccttac cgcaggggta gtctggctga
    18001 gcaataacaa gctggtcacc tccccggtaa aacaacttga aaataccctc tatgacctgg
    18061 gattcaaggg cagaagtgtc aataaggccc acaaactgat ccgcccggag gatatcgcca
    18121 tcgtggctgt ggacaacgca agcatcgatg aactgggcag ggtacaactc tggccccgtt
    18181 tgtacgatgg cctggtcatg gaaaaaatag cggcagctgg ggctattgcc atcggggtcg
    18241 acatgctgta caccgaaacg gacacgctcc ctgcaatcta cacccggatg ttgtcccagg
    18301 ccggaatcat ggattccgca aaagtcattt ctgccctcag tacggaccag gatctgtccc
    18361 gggccattga aaaagcaggc aacgtttacc tctccctttt tgacaacgat gccgattcct
    18421 ccagggcggt aagtgattca gtgatacggc ctctccggct tttgacggcg ccggtcagcc
    18481 cggaaaggaa gttctcccgg atgatctatc cccagttgcc cttagcctcg ttcacccgcg
    18541 gagcaagggc cgtgggcagc atcaatatgc cgtccgatct ggatggggtg gtccgggatt
    18601 accagctggt ccagcaggtt ggggatgaaa aagacgggca agtgcgttat ctcgccaatt
    18661 tcccttatta catgcttgcc gatctcctgg gggtcccgga atcggaggtc cgatttgaag
    18721 gcagcaccct ttgtctcacg gacagtttta aaatcccttt aaaccgaaaa ggttcattta
    18781 aaataaactg gctgggtacc aatgaagata tccggatgat ctccttttac aaggtcctca
    18841 acgacatcgt gccggcagaa tatttcaagg acaaggtggt atttatcggc gccagcgctt
    18901 cgggcctgga agacatcaaa accactccgg atgcggatga ccgcgtaccg ggagtgaaga
    18961 tccacgccgt ggcttttctc aatatgatta accaggcctt tttcaccaat ttcaattcca
    19021 tctggtttta cctggtcctc ctgctcatca ccttcctgat ttccattgcg ctgagcaata
    19081 tggaacccat gtttggattg cttttcctcg ccggggccat gctcctggag atcctggttg
    19141 ccgtagtttt tctttttcag caatggggcg tcatgatccc cctgggcacc ctgttgggat
    19201 ccactttgct ggtcagtatc ctcaccacca cctacagtta ttttaccgaa caaaaacaaa
    19261 aaagacaact gaagaacgcc tttggcacct atgtgtccgc aaccgtggtc aataaaatac
    19321 tggaggatcc caagagcctg atgctcgggg gtaccaaaaa ggtcttgtcc gtcctctttt
    19381 ccgatatccg gggctttacc aattattccg aaaaaatgga cccgcaactg gtggtgacca
    19441 tcctcaaccg atacctgtcc gccatgtcgg agcccatcct ttcccatgaa ggcaccatag
    19501 ataaatttat cggggatgcg atttttgcca tcttcggcgc cccgcttgaa ttttcggacc
    19561 atgccgacga agcctgcgaa gtcgccctgg aaatgtttca caaactggat gaagtcaacg
    19621 aatggattgt ctccaacaaa tttgaacccc tcaagatcgg catcgggatc aataccgggg
    19681 aaatgacgat cggaaatatc gggtcctcca aacggtttga ttatacggcc atcggcgatg
    19741 ccgtgaatct cggttcacgc accgaaggcc tgaccaaata ttttaatgtc agtgtactgg
    19801 tcacggagta tacgaaggat tattgtaaga gcggtcaatt cctgttcagg gccctgccct
    19861 gtaccaaagt tgccggcaaa gccgagaccg tcggcatgtt tgaactggtg gattataagg
    19921 caaaccgcgc aaaatatgaa ccgcatatca gcctctggga acaagcgctc caggctttcc
    19981 atgactataa gttcgatgag tcttatgacc tgtttgccca atgtaaccag ttgaaaaaag
    20041 acgattatgc cacggagatc tatatgaaaa aatgcaaaga gtataaggtc cacccagaaa
    20101 atttttcgaa tattcttgag atgggcagca aataagatga ttgtccagct gccgagcttt
    20161 ccaactgtcc aactgtccaa ctgactattg actattgact attgactatt gactactgac
    20221 tactgactac tgactactga ctactgacta ctgactaact tgctaacttg ccaactgact
    20281 aactaacttt gccccatgcc tcaaaaatta tttcttttgg atgcttatgc cctgatcttc
    20341 cgggcctatt acgcctttat ccggaacccc atcatcaatt ccaaaggggt caatacctcc
    20401 gccatcttcg ggtttaccaa caccttgctg gaaatcctgg aaaaacagaa acccagccat
    20461 atcgccgtgg tgtttgacca cccctcccaa accctgaggg aacaggaata caaccactat
    20521 aaagccaacc gggacgaaac gccccaaccc atcaaggacg cgggcccctg gatccgggat
    20581 atcatacgcg gcatgaatat ccccatttac gattacgaag gatatgaagc agacgacgta
    20641 atcgccaccc ttgccaaaaa aggagccgcc gccggccatg aagtttacat catgaccggg
    20701 gacaaggacc tggcccaatg tgtaacggac caggtaaaaa tttaccgccc cggcaaacaa
    20761 ggtggcccgg cggaagttct cggcatcgag gaagtgaaac agaaattcca gatcaaaaca
    20821 ccccttcagg tcatcgacct gctgggcctg atgggcgatg ccgtagacaa tattcccggg
    20881 gttaacgggg tgggagaaaa aacagcggtg aaactgatcg aagaattcgg ttccattgaa
    20941 acactctatg aacgaatcga tgaggtcaag ggcaagatca aagacaaact cattgaagga
    21001 aaagaaaacg cctttctgtc caagcggctg gccacggtgg tcagcgatgt cccggtggaa
    21061 tttaatgagg aagaactctc gatcggcgcc cccaaccggg aagtgctggc tccgatcttt
    21121 tccgaacttg aattccgcac cataggcaaa cgtttgttcg gagatgaatt cagtgtcacc
    21181 cgaaaaacca caagcccttc cgcccctacc ctgtttgaag acgttcagga agaggacagc
    21241 cccccggatc acggggtttc ggctgcatac accatccgga acaccgccca tgaatatcag
    21301 attgccggca ctccggcact ccgggcagaa ttgatctccg tcctgatgaa acagaaggaa
    21361 gtggccttcg ataccgagac caccggcctg gactccctca acacccgcct catcggcatg
    21421 tccttcagtt ttgaagaaca caaagcctgg tttgtcccgg tgcccgaatc ctttgaagag
    21481 gcccgcgccc tggtccgggc attccagccg gttttggaga acccctcgat catcaaaatc
    21541 gggcataaca ttaaatttga ctggcaggtg atccgccaat atggggtgga gattaaaagt
    21601 ccgttttacg ataccatggt ggcccattac ctcatcgatg ccaatcagcg ccacaaaatg
    21661 gattacctgg cggagaccta tctgcaatac agccccgtac ccatcgaagc ccttatcggc
    21721 aaggcaggta aaaaacaaaa atccatgtcg gaggtgccgc tggccgagct ggccgaatat
    21781 gcggccgaag atgcggatgt caccttccgg ttcaaaaaag tgtttgatcc gctgttaaaa
    21841 caaaaagaac tgacccggct ttttgaagag gttgaattgc ccctggtcga aaccctccag
    21901 gatatggaat ggcatggcgt gcaggtggat gccgcttttc tcggcgaata ttccaagtcg
    21961 ctggggaaag aactttccga aatcaaagaa aaaatttacc agcaggcggg agcggaattt
    22021 aatctcgatt cgccaaaaca aatgggtgaa atcctttttg accggatgaa aatcccatat
    22081 acgggaccta aaaccaaaac cgggcagtat caaaccaatg aagataccct gaccaaactg
    22141 gcggagaccc atcccatcat cgaatccatt gtcaattacc gcgaactggc aaaactgaaa
    22201 tcgacctatg tggatgccat tcccgaactg atcaatccaa aaacgggaag gctgcacacg
    22261 accttcaacc aaaccatcgc cgccaccggc cggttgagtt ccatcaatcc caaccttcaa
    22321 aacataccga tccgcaccga ccggggaaaa gagatccgga aagcctttat cccccgggac
    22381 gccgaacatg tgatcctgtc tgcggattat tcgcagatcg agttgcgcat catcgcggcg
    22441 atcagcaagg atgaaaatat gctcgaggct ttccgccggg gagaagacat ccataccgcc
    22501 accgcctcga aagtttttaa cgtggccagc ggagaagtaa ccaaagaaat gaggcggaag
    22561 gccaaagccg tcaatttcgg aatagcctac gggcaatcgg ccttcggcct gagccagacc
    22621 ctcaatatcg cccgcagcga agccaaagag atcatcgaca gttattttac gcagttcccg
    22681 ggcatcaaac aatatatgca ggataccata tcctttgcca aggaacacgg ttatatccag
    22741 accctcctgg gcagaaggcg gtatctgcct gaaatccatg aacggaattt taccgtccgg
    22801 gcccaggcgg aacgcaacgc catcaacagc cctatccagg gatccgctgc ggatatgatc
    22861 aaggtggcca tgatccggat ccatcagcgg cttaaaaaag aaaacttcca ctccgtcatg
    22921 acccttcagg tccacgacga actggtcttc gatgccttgc gcaacgaagt ggaagggatc
    22981 aaaccgctga tcgaagagga gatgaaaaac gccattccca atttaccggt acccatagtc
    23041 gtggaaatgg gcacaggaaa taattggctg gatgcgcatt gaggatggaa gctggaagtt
    23101 agaagctggg agctgggagc tggcggctga aagttagaag ttcccatcga tatgcttcgc
    23161 gcctttgcgt ctttgcgaga aataaccctc tttgcgcctc cgcgtctttg cgagaaaaac
    23221 aatctgttac ccctacacgc cttcgcgaga aaaaagatca atcaatagct caccggcagt
    23281 tgttttacca gtctgctgtt tatataaaac acgaccctct tcacactaaa gctcttgggg
    23341 accgtggaca cagccaggcc gttttccggg caccctgcct cattggcatc acaagtcccc
    23401 gcgtacacat tcatatcgga agaggctatg gcggcgcgga tcaccctgag cctggtcgct
    23461 gccggtgcgg atatggccac cagggtttgc ccggtaaaat ttggggcctt tgcatcgacc
    23521 tccctcaccc tggtgaaaac atcctcaaat tcctttgcat tggtgatgac gtaataagcg
    23581 gtatccatgg caccggccct ggatattttt aaatcataat tttgataata aaggaccccg
    23641 atggcgcttt ccttcgtcat ttgccgggac gcagaacaag ccgagacgag gacaatgaat
    23701 acactcaggg aaagggaatg ggtcatgctt tttgaaaatg attccaaggg aagaatggaa
    23761 taaggactgc aaaaataggg aatatcctct tcgcgtcctt cgtgggttga gccattcccg
    23821 gggcctattc cagggaattc atcaagtact tattgcccgg atcaaaggat caggtgcctt
    23881 taggcctgac cggtatccag atttcctcct cagaagacgg atcattgttt ttgtatttag
    23941 ggcccaggag ctcaaaatgc ggccgttggt ccaattcata agccgatccg ggcaaccagg
    24001 ttccaaaaat ttcttgaaaa atacggttgt ccctgctgga ccccttatac tcgaaaaccg
    24061 cgtacagccc gccttcgagg ctaaaggctt ccatatcccc gggcacccgg tcaaagtccg
    24121 aaacctccat ggccgcccat ttttcaaact cctggtccgg gctgaaattt acaaagtaag
    24181 cgggagggta aatgctcagg gagatgacct ccttattcat ggcattgaca agatctcccc
    24241 tccttggcat aaaacttttc cacaactctg cggtttgatt tccggacaag ctcatggtca
    24301 gccgcttgcc caccagtttt ttagggccga tggtttctat tctcggattc atgcttttct
    24361 tttggttgat cagggggtta atagttcagg cagttcagga aattaccggt ttgaggtcgg
    24421 gggattgcaa ataaaggtag ataaataaaa tcagtgggct gcattcaggc ctgtaagatc
    24481 aaccggtcct cgctcttttt aaaagcatca ctacaaacac catgatccag accatataag
    24541 cggcaatgga gatccgttcc cagactccca tccagggcgt cggcaggttg gcttccatcc
    24601 gggcggactg cagtccggta agcaccccaa aaacgatcat aatcaggatg gataccaatg
    24661 aatagaaacg gaaccgtttt ccgaaaactg ccgacccaaa acccatgatg agcaacatga
    24721 gcggcaccgt aaacatggta aaagcaatat ggagggtgtc cgtaagggtt cctccacctg
    24781 ccgcgaggac ttcccgttga tgcatgggcg gccagaccag acctacgatt acatagacca
    24841 tcatcaggat gcccgcgatc cgcagacgac ggtcgccctg ggccaccgac ctgacggccc
    24901 aaccaaaaac aaaaaccagc aaagaataca agctgatcaa caccatccat aaagtcctgg
    24961 tgggactgtc taccgcggag agttcgctta ccgtctggga gaaggagctg taacccggat
    25021 gaaacattgg aataaaaaca ttcatcgcta tatacagcat ggatgaaagg acaccgcatt
    25081 taagcagagt cacgattaaa gtcccgctct ttttttccag cgacaccgat tgcgtttcca
    25141 taggccttat ttgcaaagga aaggggaagg caattgaatc ccaatgattt aaatcatacc
    25201 tatggggtta aaatccgcat cctggttcag taaaaacccg aatgaagatg gagtgccggg
    25261 tcgatgcgat ttggtcctga acccagccca agtcacgctg aatccaaaac ttccgcatcc
    25321 tgatcatttc cccaccaggt gaatgatcgt tgtgttgaat ttacccgctt cttcctccag
    25381 gggttcgtgt ccggattgtt caaaccagat gatttcctta tgaggggcat tcaattgttt
    25441 cgcaaattct tcagtgattg ccgatacgag attccagtcg tgcctgccca ataaaaagta
    25501 caccgggatt tttaaagatc ttatcccggt caggtcgact tcaaacatat ccttctccag
    25561 gtgttcggcg gaatatttga aagccgcaaa ccaatcgtaa tttttataat cctcatattg
    25621 tgtttccgct ttttgaattt ccttttcggc ctctttgctg aatatcgccc ccccgtattt
    25681 actcaataat tcatgctgtt ttaaaaaaca attcagttcc cctttacata gggaagtgtc
    25741 gcctttttcc aattgatcca ggacccgcag ggtttggatg tcgtcctttc ccctggcttg
    25801 ttccgctatc caaattcggg accttttaat gctctgccgg atattcagca cctgggcaat
    25861 gccgaaatag gcggcatagt cttccgggta tttttcgatc agtttgagcc cgaccacact
    25921 tccccaggaa aagccggcca aatagatccg gtctttccag aatttttcct tcaaccaaag
    25981 agtcagttca tgaccatcct ccacgatcct ttccaaactc atgttttggg ctgcaggatt
    26041 ctgcatatat gattttccgc aacctctttg ctcccaggcg accaaagtca tggaatcgct
    26101 gatcccggca ttaaaatacc gcagatgcgg ggtctggggc cagccgggtc cgccgtgaag
    26161 gaaaagcaac accggtttgt ccggagtggt cccggtgatt tcaacatatt gtttttcacc
    26221 gccgagttcg aggaaggttt gttcattcac ggttacggaa gcggtatctg ggccgggcct
    26281 tgctttattt gatttttcca cgcaggaaaa aataaagggg caaaacagga gataaatcaa
    26341 tcgtttcacc agatttgttt aaaccaatgc aaggtaatgt tttcaggttt tgcaccagtt
    26401 gcttcttggc ataaaaccat cagcaattct ttccccccct ttacccattt atctggcttt
    26461 cctttgggag cacctgctca aatttaataa ccgggagagg aaatttatgg tgaatagtcc
    26521 attctccatg atttcaaatt cagcttcctc ctatctttga ctccttgtaa tgaaatttcc
    26581 taaagtgttt tccgccgggg agctggctgc gctgtgtgaa gcagaggtat tgggggatcc
    26641 ctccacccca ctccaaggca tgaatgaaat ccacaaagtg cagcccgggg acctgatgtt
    26701 tgtggacaat gaaaaatatt ttcgaaaaag cctggaatcg gaggcttcgg ccatattgat
    26761 caaccagaaa atacccgttc ccgcgggcaa agtcctcctt tatcatcccc aaccctttga
    26821 tgcttacaat gcgctggcct ggcatttccg cccttttatg cacacccggg aagccatcaa
    26881 cccgctggcg atgatcgatc cttctgccac gatcgaatcc ggggtcgtga tcggcgcctt
    26941 cgcccgcatt ggagccggaa cctatatcca ggccaatgct tatatcggac cgtataccga
    27001 gatcggtgaa aaggtcatca tccacccggc ttgcgtcatt ggaagcgatg ctttttatta
    27061 tcacaaaacc aacctggttt ataccaaatg gaggtccatc ggacgggtgg tggtcgaaga
    27121 tgaagtcgaa atcggatcga actgcaccat cgccaggggg gtaagcgggg ataccgtgct
    27181 gggttatgga tccaaactgg attgccaggt ccacatcggt catggggtaa gggtcggaaa
    27241 acactgtctg atggcagccc aggtggccat cggagggaag accctgatcg gggaccatgt
    27301 gaccatgtat gggcaggtgg gtgtggccca gaacctcaaa atcggcgatc acgccgtatt
    27361 gctcgccaaa accggggtat ccaaggacct gccgggaggc gttacgtatt acggaatacc
    27421 cgccgaaccg gccaacattg cctataaaca actggctatg atgcgacaac tggcccgttc
    27481 aaaaaacaaa tcatgatcca tccttactgg ataagctatt tgattgcgct ttttttcacc
    27541 ctggagcccg gagaaagacc atccgactgg ttttaccggc aaagagcctg gcctttggat
    27601 tccattccac aacagttcta ccagaaggcg gtcggcgagg ccatgctaaa agccagagcc
    27661 agctatcgtg aatacaatca ggcctggacc ttcgccggac ccatgaatat cggaggcagg
    27721 atcaccgatg tggaaggcgt gaacggcaat gccaacatca tctatgtcgg ggcggcttcg
    27781 ggaggggtat ttaaaacgac caatgggggg caagcctggc aagccatttt cgaccagaat
    27841 ccggtcttgt ccatcgggga tatggccatt catccatcca atccgaatat cctctgggtc
    27901 ggtacggggg aagcaaacgg aggcggcgga tccgtatcct atgaaggcaa tggcatcttt
    27961 aaaaccacgg atgggggtaa ctcctggatc tacctcggac tggagcaatc cggcagcatt
    28021 ccgaaaatac tgatccaccc gttcgacgat aaaatcatct atgtggccgc catgggcagg
    28081 atgtttggca aaaacaaaca gcggggcgta ttcaaatcca tcaatggggg ggatacctgg
    28141 gagcaggtcc ttttccattc ggactccgtg ggcgccatag acctcatcct ggatccgctc
    28201 catcccgata ccctgtatgc cgcattgtgg gaaaggtccc gccggccatc cggcatcgac
    28261 tatggaggcc ccgcctgcgg gatataccgc agcttcgacg ggggtaaggt ctggactccg
    28321 ctgagccagg gactccctgc cggggccaac cttggccgta ttggaattgc cctttgcgcc
    28381 tcctcaccgc aggtagttta cgcctattat atggatgaga gcggggattt cctgggtatt
    28441 taccgctccg acaaccatgg caatacctgg accaaaacca atggccgggt caatgtggcc
    28501 aatttcggat ggtggtttgg caagatctat gtatcgccgg aaaatccgga cgtggtctat
    28561 gccctgggct tgtccggaac caaatccgta aacgggggac aacagttttt acccatgacg
    28621 gaaaatttca acgaagacgt tcatgtggac cagcattccc ttttcatcga tgagaaaaac
    28681 cccaaccggc tcctgctcgg taatgacggc gggctttacc tcagccagaa tgcgggcaac
    28741 caatggtcga agatcaataa cctgcccatc acccagttct atacctgcca tatcgattat
    28801 agccagccgc aacgaattta cgggggcacc caggacaaca gcagcatgcg ccgcctgccg
    28861 ggaagccagg accagtggga aatcatttgg gaaggggatg gttttgtctg cctcgtcgac
    28921 ccctccgaca accgttacgt ttacaccgaa tcccaatacg gggtttttgt acgcagtacc
    28981 gatgggggta aaaacttttc caccgcactc aacgggatcg acggcaacga ccggaaaaac
    29041 tggaataccc ctgttgtctt caatcctaaa aacccggctt cactctatct tggcaccaac
    29101 cggctctata aatccacgga cagggccctt tcctggcagg tcatctcgcc aaacctgggc
    29161 ggaatttcca gttcaaggaa ttatggaacc atcaccacga tttccgtctc ccccctggac
    29221 agccaactga tttactgcgg tacggataac ggccaggcct gggtcaccaa aaacggcgga
    29281 caaagctggg accagatctc aacgggcctg ccccagcgct ggatcacctc ggtggccgct
    29341 gatcacaata ttaaatcaaa agtgtatatc agcctctccg gttaccggta cgatgaatcc
    29401 atgccccaca tatttcgttc ctccgattat ggaaccacgt ggaccgatat ctcccggggc
    29461 ctgccacccg tgccctgcaa ccaggtcctg gtggaccccg ccttatcggg gcatctggtc
    29521 gtcgcgacgg atgccggcgt atggcgctct tacaacgatg gcaaccaatg ggagatgccg
    29581 ggcagggatc ttccccttac cgtgtacacc agccttgcct ttcatgctcc gacccgtacc
    29641 cttgcggctg ccagttacgg acgcggcatt tatacctata ccctcgaatt gcccgtaagc
    29701 caggaagaaa cctcgcccga cccgcttgtt ttcgaagcat ttccaaatcc tgtaaaagaa
    29761 gtgttgaccc taaaattcag ttcaccaaaa actcaaaaac tgacccttgg tttgacggac
    29821 ctgggtggaa ggtttttatg gaacaaaacg atacaggtaa gaccagggct ggagtaccat
    29881 gaaaaaattc agtggaatga agggccgggt atttattttt tgaggttgca ggaaaacaat
    29941 ggcgaaacga aaactctgaa aatcctccac ccttaattcc atttcgaaaa gaaaacaggt
    30001 aagccccaat agtaaaattt gagatccggg aacatagagg ccctgcattc cggattatgg
    30061 ttaaccggag aatcgcaatt ccggacaaat ggcgattcag tacgattttc attaatttta
    30121 ttttcatgaa aacaacacta tcgcccattg ccttgctttt tcttttcgtc catctgtcag
    30181 cacaatcccc acttactttg gctgactata tccggacggc gcaatcccga tcgcttaaaa
    30241 gcaaactggc ccagaccagg acggagatca gcaggtacca ataccagtcc tttctcagcg
    30301 atctgaggcc acagatcagt ttttacggga gccttcctgc ttacagccgc gattatttcg
    30361 atgtccgcca gccggacggc accataaaat tcctgccgag gagccagaat ttttcaaata
    30421 ttgggttttc cctgagccag caaatattgg ctaccggggg aatattgtcc ctgcgcacag
    30481 accttagccg cttcgatgat tttaatttaa aaaccaaatc ctacaacagc acccccctct
    30541 accttcgttt tgaccagccg ctttttggct tcaacgaatt aaaatggaat aaaaaaatag
    30601 agcccctgaa actggaagaa tcaggaaaat cggaattgct ggaattgcag aaaatagctg
    30661 tggacatttg ccgctcttat ttcgaggtgg ctgaggccca gctgaggggt caaatggcag
    30721 aagccaacag gacgaatatc cttgaattgc tggaactgga agaaaaacgc atacccctgg
    30781 gtacgacttc ggaagaaaag attttacaat tgaggctgca actcattcag tccaggcagc
    30841 aagccgacca ggccaggtac caggtgcagg tcgctttatt gcgactgaat gcggaggcgg
    30901 gtctgcaaaa tggggaaacc gccgccttga tgctgccgga ggagctgcct gtcgtgaaat
    30961 tatcggcgga tacggcggtt tattatgcga ggaaattacg gcccgaattc ctggcctttc
    31021 aacgcagcag cctggaggcg gagagggagg tgaaaagggc cgatgccgcc cggaaatcgg
    31081 ttaacctcag tgcgagtttc ggctataata atgccgcgga tgaattcggc gacctgctca
    31141 acggcgccct gaaccaacaa aggctgtcgg tcggcatcga tctgcccctg gtggattggg
    31201 ggagaaggga tgccagggtc caggcagcaa aagctttaca acgcctgacg cagttcaaca
    31261 atgaattgaa cgaacagaat tatacgcagg agatcatcac ttcagtgagt accctggaat
    31321 tattacactc caatatacag gtggccaggg agacggagtc cattgccagg gagcgattcg
    31381 aggtatccct caggcaatac cagaccggac ggctcggcct caccgagctc aatattgccc
    31441 gtcaggaaaa ggatgaagcc agggtgcaac acatacaggc cctgagaaac tggtggtcgg
    31501 gctattacga actgcgtcaa ttgaccttgt atgattttga ggcagaaagg gaaattcaaa
    31561 tattagataa gtgaaaaatg acccaatcgg cttggaatta aagttaaaaa ttcaatggtt
    31621 tcc
//
LOCUS       DAOQXT010000051        31613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1684_length_31613_cov_5.871221, whole genome shotgun sequence.
ACCESSION   DAOQXT010000051 DAOQXT010000000
VERSION     DAOQXT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 31613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31613
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1684_length_31613_cov_5.871221"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1477)
                     /locus_tag="PKM27_10350"
     CDS             complement(161..1477)
                     /locus_tag="PKM27_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR07703.1"
                     /translation="MKKSLLFYLCLVTALPVWAQKVYTLEECIEIAIKNSLLTQQAQL
                     NLQDAGTDITQARHNKYPDLNLSIGHGLNFGRSIDPSTNDFINQQITRGGFYAGSNVP
                     VWQAGRFNQAVRQYQYAEEASRWALKNQEDNLRLQVTLAYLLVLNNLDQAKRLQIQEE
                     LTAQQLLRLEDLDKNGAALPGAIADMRGQLASDQISRLDAEQQISTSKLSLAQLMNIP
                     FDLNMQIAPDNRTGTRETPESARTLLAETMNYHALIRSGEFTQQSVQSFIQSLKASRY
                     PQVGIGMEMGSNYSSAYQIQGSKVKFTKQLSNNFNYAVGLSLSVPILNNYRIRSSLQK
                     AQTLLQRSKNDIAQARNLVQQRIEEALLLQANAGERVRILQNQVDAFRESFRIAEVRF
                     NQGVLNSVEYLVVKNNLDRSENQAITARYELSFRDKVVEFYANLKQ"
     gene            complement(1494..2156)
                     /locus_tag="PKM27_10355"
     CDS             complement(1494..2156)
                     /locus_tag="PKM27_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR07704.1"
                     /translation="MIDLQHIFKWVPVGGARNFLLRDLSLQIGEGEFVSIMGPSGSGK
                     TTLLNVIGMLDHFDEGEYKFLHEEVHKMKEKQRTQLYKQYIGFVFQAYHLIDELTVYE
                     NIETPLLYQDVKGSERKALVADMLDRFNIVGKKDLFPNQLSGGQQQLVGIARALIAKP
                     KLILADEPTGNLNSKQGEEIMNIFSELNQQGVTIIQVTHSEKNAGYGSRIIELLDGRI
                     VN"
     gene            complement(2365..2811)
                     /locus_tag="PKM27_10360"
     CDS             complement(2365..2811)
                     /locus_tag="PKM27_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR07705.1"
                     /translation="MKKFLFLVVFCFPLLVTGQESADRLAAEKAMMNYLEGFYEGDTL
                     KLAESLRPGMYKTGYWKNKSSGKYDFDGRMTYDEAIQYARNVLIKKNFAKPGSPKKVE
                     VLDISKHTAAGKVYAWWGSDYILLSRQDGRWMIDQVLWEGPPETKD"
     gene            complement(2822..5197)
                     /locus_tag="PKM27_10365"
     CDS             complement(2822..5197)
                     /locus_tag="PKM27_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07706.1"
                     /translation="MFSNIFKLIFRNLYRYKYYSIVNILGLSIGVAALVWAWQTYRFS
                     FSYDRFHQDRDQIFRILSKIEGRDVKLGVCPLPLGPMAKADLSAVSDYVRLENINLSL
                     KAGQEEALNSRVTYTDPSFFDFFNFPLVAGVHEIGDRSNILLTETSAKKIFGTKDPLG
                     QTVLLHAGESYQQPMKVAGVLKDPPVNSSIQFELITNMENYVKNDSALLKADDWTWLA
                     DAIFLKIPDPANAVRVEKELTKYAPIQAKARQDLKAEHFILDPLDEVANHDRDIASNV
                     LSQRPEDSAAYGPLVLALLILISSCLNFANTTVARSNQRLKEMGVRKVLGGNKMQLIR
                     QSLLECGLMVFLAVLLSTLLNSWWLPVFNNMFTGIKISADYFSDPVLLRTMFWLWLGV
                     TLIAGLYPAFYISRYNATQIFRGSVKFGGTNLFSRVLLGLQLLVACITVIAGFAFSRN
                     SDFQKNFDFGFAVDDVFSVQLNKNTFDAYHQALSQAKSVEALAGTRGHISSSYKFIGL
                     EAKGEKKETRLLEVGDRYEEVMELQLLSGRWFKPFSTADLDHTIVLSEKAASEFGWTA
                     ETALGQQVKWDTVRAQVIGVTKDLYMTAFFDPIEPVAFTKVQPSAYRTLVVKSRPGQV
                     NPAFNEARAIWSGLYPMKPFNAFYQNEIAAEAHRVNASIATIFFWFAIISALLTATGL
                     FALISLTILKKLREIAIRRVVGASPGHIVFIVNKSYILIFVLSLVLGVFAGIALTRLL
                     MDLIFVVNVGVHGPTLVYSIAGILLLVAVTLGIKIWHTLSLKPSEVLRANQ"
     gene            complement(5228..7633)
                     /locus_tag="PKM27_10370"
     CDS             complement(5228..7633)
                     /locus_tag="PKM27_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07707.1"
                     /translation="MFRNYLLTGIRDLWKFRFFSGINVFGLGISISACLLVIMLIRDA
                     YNYDRFHPEPNRIYRVLTYAQRKGGGTEAYASSPYALSSFMAAQSSSIKEWVPLINRF
                     NGELVLGESRFQFEGLGTHPSFFKTFGFSLEEGNAGDLNQPNVIFLTAGLARKIFPDG
                     NALGQNLVWGGHQQALRVAGVLKEFPGKTHLEFDALTSVETLSAATGADAFGFDKNNW
                     LDYYAGWHFYRLTDQADKSKIEAELNEVAQNRYSGLHLESRDAGYSFKLQPMDKITPG
                     PLLSNSIGRGMPSVLLWFLSILGILIVASAGFNHANISLARAVNRAREIGIRKINGAG
                     RLNIFSQFIVESILVALLSTALGILILQWLIPLFSRLDFLNSMDITLRMDWALYGWYL
                     VFALVVGVLIGVLPAMMISRVAILPSIQKLSGLRILPQVSLRKAIITGQLAMTLVFFF
                     LITTSYLQIRFALDTNFGAGQDYMVNLDLQGQEYLRVKTEMSKLPVVKSIAASSMLMG
                     TYRDSKVDVKTKDTADALGVRDYVVDENFLPAFGLTLLNGKNFEPDPDQQHEKQVLVN
                     ESFLRFFNMGDPSEVVGKGIFLDTSLVSIAGVIRDFRFKPADYAIEPLVLRYIPEQFN
                     ILHLTLDRSADLQQAVASIRMSWAALDPGRPAQWSFYADDVKATYRSLRDILWIIAFF
                     AALAASITLMGLLGMVTYILSGRRKEIAIRKVVGAGLDKLFWVLSGSFIRWLVLAVLI
                     SIPLASLLNNLWLQNYAYRVTWSPLWFVPGLALLTTAVLLIVGLQVLRAALSNPVSSL
                     RNE"
     gene            9024..9974
                     /locus_tag="PKM27_10375"
     CDS             9024..9974
                     /locus_tag="PKM27_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR07708.1"
                     /translation="MKVKFWGCRGSLPASVTHDNIEYKVRFALEKAIRLGVQSSSALD
                     EFIRDLPFHIRSTYGNNTPCVEILGGNEIVVCDAGSGLRDLGRYLVSQPFSGPRVVNI
                     LVSHLHWDHIQGFPFFIPAYMQGTVIRIWGCHDGLESAFVNQQNPPCFPVLLEDMGAN
                     IRFHPLDLHGPNQIGGLKVTAIEQPHPGLTYGYCFEQDGKKIVYSTDIEHQDEVEDPM
                     NPYVQFYRDADILIMDCQFNLADHLYTKQNWGHSSNLIAIELGVISKAKTLCLFHADP
                     FLDDLQLDKFLQDSRRYHQIYAPDSEMEILLAYDGMEVEC"
     gene            complement(10174..12009)
                     /locus_tag="PKM27_10380"
     CDS             complement(10174..12009)
                     /locus_tag="PKM27_10380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNR07709.1"
                     /translation="MRKPGIKGIKPGGALICCLFCAVLFAQVQPLSRNDSAQVLLAEE
                     NFRNELARNNLKQASHYLNTIARIYWDHNQTRKAIEYYERSLELNQGLGNESGAAMIR
                     GNLGLLYADLRDYRRSFEEWQKVLATRRSFGDKENIIEALINSAVSLNSLRRYEESVR
                     FLEEAADISRQINNRERMLDNLLRCYSYLSETYEKAGNPALSRQYYDQYRTFSEEKKR
                     IDIRRMTNALDEQTYLKELAEAEEKKKAQELINKQKELLYLALAKELLDSTNQVLDTN
                     LAMAELNLSLLRKQRQSDSTIISRDKEIIRQQKTVQRSLGLASISLLAVCFVIYLNFI
                     QVKRSKKILAEKNGLIEKQNRELEDLNRIIAAHNLRMKKELDVGQEIQMSMLPKIYPP
                     GNLLDMHALLDPAREVGGDLYDFFMTDDEHLLFGIGDVSGKGVPAALFMAVTKTLVKA
                     HGIKMDSPAAILSAVNEDLQAENEKSMFVSYFLGKLNLRTGSLVYSNAGHLPPIVRNK
                     QGSKKLEGIHGPVLGAVDAFHYQEGRITLEPDERLLLYTDGVTEAMNIRQEQYGEDRL
                     MKFIDHKSSGGDSRTLVDALRQDLSRFAGNEEQSDDITMIAMARK"
     gene            complement(12006..13757)
                     /locus_tag="PKM27_10385"
     CDS             complement(12006..13757)
                     /locus_tag="PKM27_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07710.1"
                     /translation="MKSIRIQNIFPAGLAGSFLMLCCQVLVAQPGPPVPRYFIGPDST
                     LYWNRALPVYIKLAPSPDGAGVNLVSKEFEKYTNPVFLDTEGPNFIRTRYAVDKNTRQ
                     TVKPQLEVLFEVVADGEAPESTISFKNANAFSSAGKQYYGRGLTYELTSSDKYAGVEF
                     IHSAINSDPFSVYQQAISVPGEGEQTVRFYSTDRVGNVEPVKQSVFTTDLSAPVISHN
                     INGISDSNTIASTSTIYFTATDPLSGVARIHYRFDQAPFSIYTGANLKLDHLEDGDHV
                     LEYYAEDHVGNRSVNAGFRFYYDKLAPITASDILGDKYIWQDKVYFSGRTKMKLTAID
                     NKAGVKEIRYSVDQEAFKTYEEPFYLPAVQGNHTIRFYSIDRLSNTPGGAEEYKHNIN
                     LVFLDLTGPDIGHSFNGPTFRANNELFMGPKTTFVLQGKDNQSGLQYMAYSIDGAQAE
                     TRYEKPFGLDLPSGVHRIELFAYDQVNNRNVSEATVHFDSGSPIIISNFSTSPSAQRD
                     GLGVYPPYVVLFLAATDEIVGNDKIYYRINDGPELAYSNPIGNLAPDKKYEIRVRAVD
                     KVGNESTRSVSFFTSAR"
     gene            complement(13754..14179)
                     /locus_tag="PKM27_10390"
     CDS             complement(13754..14179)
                     /locus_tag="PKM27_10390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR07711.1"
                     /translation="MTIEITNQIKEINRVHRSLDRYLREKSLGEYDRKKVSIAVDEIL
                     SNIISYAYQDQGRHLIRINLECTEDKLILEFLDDGKPFDPIEFIRNTEGRSAEDLDRI
                     GGLGLKLVGRLMDRLDYLRQGNQNKLTLELNYSKNYIDS"
     gene            complement(14197..14532)
                     /locus_tag="PKM27_10395"
     CDS             complement(14197..14532)
                     /locus_tag="PKM27_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNR07712.1"
                     /translation="MEFEIQNLDHVKIISLSGDLNANTAGEAETKLIQLIMGGNRKLI
                     VDLEQLNYISSAGLRIFLAANKLVKKMDGEIRFCSLNRTVLEVFDISGFNMIFKFYPD
                     QASAIQGFH"
     gene            14709..16064
                     /locus_tag="PKM27_10400"
     CDS             14709..16064
                     /locus_tag="PKM27_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNR07713.1"
                     /translation="MKLLLSSLLLFLFIYPGCKAPEKETAAGPDRKALADTLETLLKQ
                     NLLHPWYPAAVDSVHGGYLSTFDSAFHPVGEQNKMIVSQGRHLWNNSRAALHFPNDRP
                     YKAYARHGFEFLRDKMWDPQYGGFFTLVDRMGNPQGEGSKTAYGNSFAIYGLSAYFLA
                     SADSGALDLAKKAFLWLESHAHDSVHLGYFQHLSRQGERQPRLPTTPSTSDLGYKDQN
                     SSIHLLEAFTELYQAWPDPLVKTRLDEMLFLIRDKIVNEKGNLVLFFQPDWTPVSFAD
                     SSRESILRHHNLDHLSFGHDIETAYLLMEASHISGWENDSVTWARAKQMTDQCLRYGW
                     DDTVGGFYDEGYNFKDTPGTTIIRDTKTWWAQAEALNTLLIMADLYPDDPLQYFDKFL
                     KQWSYIDRYLIDRQNGDWFDSGLDKNPAAAGRNKGHIWKALYHQYRSLENCVVRLRGD
                     Q"
     gene            complement(16239..17237)
                     /locus_tag="PKM27_10405"
     CDS             complement(16239..17237)
                     /locus_tag="PKM27_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR07714.1"
                     /translation="MAAPLPDFYDKFITALKLKGPAQNILVKELGELLHLQNRAIYNR
                     LNGDTQFTINEAILICKTYNIDIQEILLPDSDSIIYRSATIDRKINAPIEYLQGLVFY
                     LEWLKTLSNPKIHYTSHDFPLFLTCAYPRLIKLKLAIWSYYTWNLKSFTSATVLDLED
                     KEFSGVDEICKKIVGLYSRVPSIEYLPSSLFDNTIYQIRFLAEVQGFLHSDQIRLLIR
                     DLESMIALMEEIANSGTKNPGDVQFYRSDMLNQSLNFMVEWDHGCRVYTMIDSPNYIY
                     TESPRMTSHMKEYFKKIVNSSESLTQSNAKERRKYFNMLFQKVKALGEFGLSSASQ"
     gene            17700..18296
                     /locus_tag="PKM27_10410"
     CDS             17700..18296
                     /locus_tag="PKM27_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07715.1"
                     /translation="MNLFRNLSICRWMTYCILACLANPRVQAQDQLSISLFNHATTLP
                     PKGFSGPLHPGIDIGISTHLKPKENTLSFFHWKLGYYYHRLVHQGIQLYGEYNKQYKI
                     LKPISLGWSGGLGYLHTFELHEKFILGGDGTYRRTGRLGKPHAQASLALQILGRWGYW
                     APFAEYRLRLVSPFVNKYVPLLPSTSLHFGAYFNFHTI"
     gene            18308..19498
                     /locus_tag="PKM27_10415"
     CDS             18308..19498
                     /locus_tag="PKM27_10415"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR07716.1"
                     /translation="MKKLMIILGLCLSALACQKGDIDASTACSFTYPPGNDNHPKAAR
                     LRDILNKYNKLGLPGISLLVRDGSGSWIGAAGMADIEKNIPFQPCTVAKVASITKMFT
                     GTLVHLLVEQHTFKLDDKVDAFLDEEVLDKVKNCRGATIRQLMNHTTGIYDLITSDAF
                     YLALLNDPDKKWSAEELIKFTYGKEPKFELGKSCFYSNTNTLLLSMVIQKATGRTNQD
                     LLREKILHPLGMNDTYYYSHDELPPITAQGYFDLYNNQRIVNVTNFNTGSGNGYGGFF
                     STVLDLQKFIEPLLRTKTILTAGSMVEMTNFIPEADPEDPANDLNLGAGLMKRYFNQP
                     LTSDKYGYGHTGRDLGYSANAFYFPNYDVTCCFMVNYGTNAESELREVFFDFQDELTD
                     ALFN"
     gene            19599..20087
                     /locus_tag="PKM27_10420"
     CDS             19599..20087
                     /locus_tag="PKM27_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07717.1"
                     /translation="MKPLALKNHFFILLWLIPQMVFAQVELNTTKLTLLEGKFEKGSR
                     IIEFKPSSLIIKVKMKNLNRFTEQEIPAEAIKDITIKYDLSPVRGAMRGLMIGGGLGL
                     LIGSGLYYSGPVGAFVAFAALGTGIGLGAGGKKITRFPINGNLNEYQKNLDEMQRLLI
                     LK"
     gene            complement(20246..21721)
                     /locus_tag="PKM27_10425"
     CDS             complement(20246..21721)
                     /locus_tag="PKM27_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HNR07718.1"
                     /translation="MTPSQKFLEKHGIKDTEPDALPMEQPAEKATQNDRRGFLKKSLG
                     GLALGSFMFARPEETLEHATSRVNRFSAPSDLKITDMKYAVVMNGGGRCPVIKLETNQ
                     GLVGLGEVRDGADWRYALFLKSRIKGMNPCSVEQIFKRIKQFGYHARQGGGVCAVEMA
                     LWDLAGKAYNVPAYQLLGGKYRDKVRLYADTPQVKDPVAFAERMRVRLKEKGFTFLKM
                     DFGIELVRDIPGTIVNANFWDVNRQWSNDPMSYGAMLHPLTQVQLTDKGLEEMANYVA
                     KVREVVGYEVPLASDHYGHFDLNNAIRLGRAVEKYRLAWLEDMIPWQLTSQLKEIKTA
                     IETPLCTGEDIYLKEEFIKLIDTQSVDIVHPDLATSGGLLETKKIADYAEERGVASAM
                     HFAGLPISFMANVHCAAASMNFMGLENHSIDVPYWQNLVKTVDGKPLYEMGFAAVPDT
                     PGLGIELNEEEMKKHLAPENTNFFGPTPEWDNIRSWDRLWS"
     gene            complement(21769..22182)
                     /locus_tag="PKM27_10430"
     CDS             complement(21769..22182)
                     /locus_tag="PKM27_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR07719.1"
                     /translation="MENFTVFLSENTTLMRISRFEDILAWQKGQDLAVEVYLTLHEIK
                     DWDFRSQINRAAVSVSNNIAEGFDRHTNKEFIKFLTVARASCSEVKSMLYLAERLGYL
                     EKHKSIQLLLLTVEISKMINGLIKVLRKPYNSTSN"
     gene            complement(22281..24707)
                     /locus_tag="PKM27_10435"
     CDS             complement(22281..24707)
                     /locus_tag="PKM27_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07720.1"
                     /translation="MASFNFKLAVRNLLKNRLHTGINIIGLSIGIAAVILIYRIVDHE
                     ASYNKQFSNYDRIVRVVREEISKEDGTGYTPGLPIPAMDAVKRTVPQFEATARIRETW
                     STIAVPDPSSGLPLKKFDVDEGQIAFFTEPSFFKIFDWKWLTPDQSALLDQPNHLILS
                     RAMAEKCFGGTDQAVGQWITLDNLQPSMKVIGVVEDAPAASDFPVVFFISYPTIEAHP
                     EFYFYSKEWGSTSSNNQLFALLHSRDQMEAANQALASVGKEEYKNEQVAKKHFAQPLA
                     DLHYNEDIGTSGSHVMDKKKTWILSMIGVLILVMACFNFINMSNAQSVVRAKEVGIRK
                     TLGVGHGRLMANFLTETGILVGLAILLGTGLAYLTSPWLQYISPLPADIALFQGGNLF
                     VFLSLLFVAVTFLAGLYPAAVLSSFQPVQIFRANFEKGIGSGVFLRKVLVVLQFVIAF
                     GLIISTVIALSQLSFIRNMDLGFNKDLVYTFGFSNDSVSQSKLNTLKNQIKGLPGTEL
                     VSLSSDRPSSGNTWSSNWALQQGKEDAPFPISTKFCDEDYQETYRIKLLAGRWFLPSD
                     TAREAVINRTALKKLGITDFNSVLGNVIKLGNRTLNLVGVAEDYHSHSAHEPLEALLM
                     TSRKAYYFVAGVKIRPQNIQETVASIEKIYNNLFPEQVFKGRFYDEEIQRFYEADTQF
                     AALCKGFAVLAILISCLGLFALASFAISRRIKEIGIRKVLGASTRSIIGLIGKDFVLL
                     VLIAFVLATPLTWYLMNQWLQDFVHRTSISWWMFIAAMVLVVALALFTVAIQAVKAAW
                     MNPVKSLRTE"
     gene            24921..25409
                     /locus_tag="PKM27_10440"
     CDS             24921..25409
                     /locus_tag="PKM27_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNR07721.1"
                     /translation="MPVAEPPRERTDYEIERNKPMPSLNHAIIQGNLLFQLHLKVQKK
                     YSFLPEVSLSLPGIPVAVPDISVYPKLTADFLHDIGTMTESPWSIIEIISPSQSTDEV
                     LLKFERCFNAGVRSCWLVIPGLKAIEVYAKTDHYPFYTSDMLLQDKELDIELPMQTIF
                     GF"
     gene            25485..26657
                     /locus_tag="PKM27_10445"
     CDS             25485..26657
                     /locus_tag="PKM27_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR07722.1"
                     /translation="MKYISRPVWILSFVSLFTDTASEMLYPIMPIYLQTIGFSVVLIG
                     ILEGLAEATAGLSKGYFGRLSDLRSRRVPFVQAGYAFSAVSKPMMALFTFPWWIFLAR
                     TLDRLGKGIRTGARDAILSDEASPRTKGKVFGFHRSMDTIGAVLGPALALLYLYFHPG
                     DYKTLFILAFIPGLLAVLATLLLKDHTHKAVRTPISTNFFSFMRYWTESPSQYRKLVT
                     GLLIFALFNSSDVFLILKAKQAGLDDSLVIGVYIFYNLVYALFAFPIGFLADRAGLKP
                     VFIGGLVLFSIVYFGMAFNTNFIVFIGLFFLYGIYASATEGISKAWISNICDKKDTAT
                     AIGTFSGFQSICTLLASSLTGMIWFQFGATTALLTTAIATVILLIYFKTVPGPEKS"
     gene            complement(26691..27752)
                     /locus_tag="PKM27_10450"
     CDS             complement(26691..27752)
                     /locus_tag="PKM27_10450"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNR07723.1"
                     /translation="MIYQDSLFYAAFPSVVKRPNREFLLAFRRAPNLQAMGEKNYEHI
                     DPNSYLVTIRSRDGEVWTKNPDLLYAHPFGGSQDPCLLQLRDGSLICTSYGWRPVSQE
                     AYDKLKKPVFYSIPSKAIFLGGYVLRSTDGGHQWKGPYYPMRVPIDPKLDIFGNPLPA
                     YNRGGLWETKDGRILWVVAAGNEDNRGSAHLLVSRDKGLSWKYSSVVAEDEQAEFNEA
                     SVLETPGGDLVAFLRTANYDDQACIARSKNGGKSFSWQGMGFKGHPLHALPLPDKRIL
                     ITYGYRHKPFGIRTRILNAECTDFATAPEIILRDDGGGTDLGYTWSVLLNNNRVLVCY
                     YFNIDAGLRHIAGTIIEIK"
     gene            complement(27893..28753)
                     /locus_tag="PKM27_10455"
     CDS             complement(27893..28753)
                     /locus_tag="PKM27_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR07724.1"
                     /translation="MKKFSLLFLFGFTAIPLFGQKKLTPPEIGVVQNIENDRLLYKFG
                     FRYLVESAQKMLSPRSVSDRQFQEHLLSIKKLRVPLYACNLFIPGDLKVVGPTIDEEA
                     VLTYVDQVVRRAEAAGIKMLIWGSGGSRGVPGSFNRSEAEEQFIRMAKKIASLAEKQN
                     IILALESLNRTECNFINTIPEALAVVKAVDHKNFRLSVDIYHMLMEGESPEAITGTKD
                     YLVYCEVAEKVGRTPPGVHGEDLVPYFAALRKEGYQGKIVLECRWKDLETQGPAAYRE
                     LRSQVDEAYK"
     gene            complement(28904..31264)
                     /locus_tag="PKM27_10460"
     CDS             complement(28904..31264)
                     /locus_tag="PKM27_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07725.1"
                     /translation="MFRNYILIAWRNLLSNKTFSLINILGLALGMACSLVIVLWLNDE
                     LKKDRFHEHDARLYRVMENQYYSGDISTFSSTPGILAEHIVKDVPEIQYASQMLWEEE
                     PLFTVGNILDKEKGRYVQKDFLKMFSFHLKTGDKNSALDRPDGVVISQKLADKYFPGQ
                     DPMNKIIRIDNADNVVVTGVLDEIPEASSLTFEFLMSYDRWFKNNDWAKEWGNNGPRC
                     YVLLAENADLGKVNAKIKDYIKTKNKESNVELFLQNYGESYLYGEWKSAKQTGGRIEY
                     VRIFGIVAVIILLIACINFMNLATARSLKRAREVGVRKVVGARKSQLVQQFLSESILV
                     SFIALAFALGIVFLFLPSFNTLTQKELGIKLANGSYWALLLGLALVTGLVSGSYPAMF
                     MSALRPITILKGLLKFNPGAAFLRKGLVVFQFALSIFLILGMIVIYRQISFINNKNLG
                     FGKENLIFMPLEGDLKKSYYTFKEELLRQPGIKGVSASQSSPMEVGSSTQGVHWPGKD
                     TTQLTLFSHNPVTLDYISTMGIELADGRDFDPSFSTDTLNFLVNEAAARKIGYTDPVG
                     KELTVWGDKGQIIGLMKDYHFNSLHVPIEPLILRQFKGSWGLWGNVIVRTEAGKTKEA
                     IASMEKVFKQFNPAFPFKYSFTDEEIAKTYRSEQTIGKLSKYFAFLAIFISCLGLFGL
                     AAFTAEQRTKEIGIRKVLGAGVGNLVSMLSRDFIILVLISALIAFPIAWYFLNDWLKQ
                     YTYRENMHWWYFGLAGFLALAIALITVSFQAIKAALMNPVKSLRTE"
BASE COUNT         7872 a         7928 c         7700 g         8113 t
ORIGIN      
        1 gcgccctatc ttctaacttc taacttcgag cttctaactg gaagctggga gctggtagct
       61 gattactgaa tgctgattat tgaccgctga ttgctgaccg ctgattattg accgctgatt
      121 gctgactatt gactattgac tcctgactct tgactcctga ctactgctta agattcgcat
      181 aaaactccac gaccttatcc cggaatgaaa gttcataacg ggccgtaatg gcctggtttt
      241 cggaacggtc caggttgttt ttgactacga ggtattcgac tgagttgagc actccctggt
      301 taaagcggac ttcagcgatg cggaaggatt ccctgaaagc atccacctgg ttttgaagga
      361 tccgcacccg ctctccggcg ttggcctgaa ggagtaaggc ttcttcgatc ctttgctgga
      421 ccaggttcct ggcctgggca atatcgtttt tggaacgctg gagcagggtt tgcgcttttt
      481 gcaggcttga cctgatccgg taattgttca ggatgggcac ggacagggac agtcccaccg
      541 cataattaaa gttgttggac aactgtttgg taaatttcac cttgctgccc tggatctgat
      601 aagcgctgga atagttggag cccatttcca tgccaatgcc aacctgggga tacctgctgg
      661 ccttgagcga ctggataaaa gactggacgc tttgctgggt gaattccccg gagcggatga
      721 gggcatggta attcatagtc tccgccaaaa gcgtgcgggc cgattccgga gtttcccggg
      781 taccggtcct gttatccggc gcgatctgca tgttgaggtc gaaagggata ttcatcaact
      841 gggcaaggct tagtttggaa gtgctgatct gttgttctgc gtccaggcgg ctgatctgat
      901 cggaggccag ctgtccgcgc atatccgcga tcgccccggg cagggctgct ccgtttttat
      961 ccaggtcttc cagccgaaga agctgctggg cggtaagttc ttcctgaatt tgcagacgtt
     1021 tggcctgatc caggttgttc aggaccagca gataggcgag agtcacctgc aatctcaggt
     1081 tgtcctcctg gttttttaat gcccaacgtg acgcctcctc ggcgtattgg tattgacgta
     1141 cggcctggtt gaagcgtcct gcctgccaga cgggaacatt ggatcccgca taaaaacctc
     1201 cgcgtgtgat ctgctgattg ataaaatcgt tggtagaagg atcgatgctc cggccgaaat
     1261 tcagaccgtg gccaatgctg aggttcaagt cgggatattt attgtgcctg gcctgggtaa
     1321 tatccgtgcc ggcgtcctga aggttgagct gggcttgctg ggtcagcagg ctgtttttga
     1381 tcgcgatctc tatacattct tccagggtat acactttctg ggcccaaaca ggcaaagcgg
     1441 tcaccaggca taaataaaac agaagggatt ttttcataaa aaaggttgcg cttttaattg
     1501 acaatccggc catccagcaa ttcaataatc cgggaaccgt aacccgcatt cttctccgag
     1561 tgggtgacct ggataatggt tacgccctgc tgattcagtt cgctgaaaat gttcatgatt
     1621 tcttcgccct gtttgctgtt caggttgccg gtcggttcat cggcaaggat gagcttgggc
     1681 ttggcgatga gcgccctggc gatccccacc agttgctgtt ggccaccgct gagctggttg
     1741 gggaaaagat ctttctttcc cacgatgttg aaacggtcga gcatatcggc cacgagggct
     1801 ttgcgttcac tgcctttcac atcctggtac aaaagagggg tttcaatatt ttcatagacc
     1861 gtgagttcat cgatcaggtg ataggcctgg aatacaaaac cgatgtattg tttatacagc
     1921 tgggtgcgct gtttttcctt catcttatgc acctcttcgt gtaaaaactt gtattcccct
     1981 tcatcgaaat ggtccagcat gccgatcacg ttgagcaagg tcgttttgcc ggaaccggat
     2041 ggccccatga tggaaacaaa ttcaccctcc ccgatctgca ggctcaggtc ccggagtaaa
     2101 aaatttcggg cgccgcccac aggcacccat ttgaaaatat gctgcaagtc aatcatttta
     2161 gtttgtcagt taatcagttt atcagttaat cagttaatca gttaatcagt ttatcagttg
     2221 gtcagtggtc agaagtcaat ctcgcatctc gcgactcgct tctcgcattg gtaatattgg
     2281 tcctttaacc cttaaccctt aaccattcac ccatatttat caaggttagt ttatcagttt
     2341 atagtttatc agtttatcag ttggttagtc tttggtttca ggtgggcctt cccaaagcac
     2401 ctggtcgatc atccaacggc cgtcctgccg ggaaagcagg atataatcgc taccccacca
     2461 ggcataaacc tttcctgccg ccgtatgctt gctgatatcc aggacttcca cttttttagg
     2521 cgaacccggt ttggcaaaat tctttttaat caacacattc cgggcatatt gaatggcttc
     2581 atcatacgtc atcctcccat caaaatcgta tttaccgctg gatttgtttt tccaataacc
     2641 ggttttatac atccctggcc tcaggctttc ggccagttta agcgtatccc cttcataaaa
     2701 accttccagg taattcatca tggctttttc agcagccagg cggtctgcag actcctgacc
     2761 ggtgaccagg agcggaaaac aaaaaaccac caggaacaaa aatttcttca taactaaaaa
     2821 tttattgatt agcacgcagc acttccgaag gtttaaggct cagggtatgc catattttta
     2881 tacccagggt tactgcgacc aggagcagga tccctgcgat ggaatatacc agggttggcc
     2941 cgtggacccc aacattcacc acaaagatca ggtccatcag caaacgggtg agggcaatgc
     3001 ctgcaaagac gcccagcacg agggataaaa caaagatcag gatataactt ttattgacga
     3061 tgaagacgat gtggccgggg gaggcaccca ccaccctgcg tatcgcgatc tcccggagtt
     3121 ttttcaggat ggtcagggag atcagggcga ataaaccggt ggcggtaagc agggcggaga
     3181 tgatggcaaa ccagaaaaag atggttgcga tgctcgcatt cacgcgatgg gcttcggcgg
     3241 caatttcatt ctgataaaag gcattaaagg gtttcatggg gtagagtccc gaccaaatgg
     3301 ccctggcttc attaaaagcc gggttcacct ggcccggcct gcttttgacc accagggtcc
     3361 ggtaagccga tggctggacc ttggtaaaag caaccggttc tatcgggtcg aagaaagccg
     3421 tcatataaag gtctttggtc acgccgatga cctgggcacg gacggtatcc catttcacct
     3481 gttgcccgag ggcggtttcc gcagtccatc caaactcgct tgccgctttt tcactcaaca
     3541 cgatggtatg atcaaggtcc gcagtggaaa aaggcttaaa ccatcttccg gacaataact
     3601 ggagttccat cacctcctcg tagcggtccc ccacttccag taaacgggtc tccttttttt
     3661 ctcctttcgc ctccaggcca ataaacttgt aactggagct gatatgcccg cgggtgccgg
     3721 ccagggcttc gacggatttg gcctggctga gggcctggtg ataagcgtca aaggtatttt
     3781 tattcaactg gactgaaaaa acatcatcca ccgcaaagcc gaaatcaaaa tttttctgga
     3841 agtctgagtt tcttgaaaaa gcaaaacccg cgatcacggt aatgcaggcc accaagagtt
     3901 gaagcccgag caggaccctg gaaaacaaat tggttccgcc gaatttgacc gaacccctga
     3961 atatctgtgt ggcattgtac cggctgatgt agaaagccgg atacaggccg gcgatcaggg
     4021 tgacccccag ccagagccaa aacatcgttc gcagcaaaac cgggtcgctg aaatagtccg
     4081 cagaaatttt aatcccggta aacatattgt tgaagaccgg cagccaccag gaattgagca
     4141 gggtggacaa caagacggcc aggaatacca tcaggccgca ttccagcagg ctttggcgga
     4201 tcaattgcat tttgtttccc cccagcacct tgcgcacgcc catttctttg agccgctgat
     4261 tggagcgggc tacggtggta ttggcaaaat tgaggcagga agaaatgagg atcagcaggg
     4321 caagcaccag ggggccgtag gctgcggaat cttccgggcg ctgggacagg acattgcttg
     4381 cgatatcccg gtcgtgattg gcgacttcgt ccagcgggtc gaggatgaaa tgctcggctt
     4441 tcaggtcctg tcttgccttg gcctggatag gggcatactt tgtcagctcc ttttcaaccc
     4501 ggacggcatt cgcgggatcc ggaattttca ggaagatggc atccgcaagc caggtccagt
     4561 catccgcttt gaggagggcg ctgtcgttct ttacataatt ctccatgttg gtgatcaatt
     4621 caaactggat ggaagaattt acaggcggat ctttaaggac accggctact ttcattggtt
     4681 gctggtagga ttctccggca tggaggagta cggtttgacc cagcggatcc tttgtcccga
     4741 aaatcttttt cgctgaagtt tccgtcagaa gaatattgga ccggtccccg atctcgtgga
     4801 cgccggcgac caggggaaag ttgaaaaagt caaaaaacga cggatcggta taagtcaccc
     4861 ttgaattcag cgcttcctcc tgcccggctt tcagggaaag atttatattt tccaaccgga
     4921 cataatcgct tacggccgaa agatcggcct tggccatagg ccccaggggc agcggacata
     4981 cgccgagttt gacatccctt ccttcgatct tggacaggat gcggaagatc tggtcccggt
     5041 cctggtgaaa gcggtcatag ctgaaactga accggtaagt ctgccaggcc caaaccaggg
     5101 cagccactcc aatcgacagt cccagaatat tgactatgga ataatactta taccggtaaa
     5161 ggttgcgaaa aatgagtttg aatatattgc taaacatttt acaccgattc agaattataa
     5221 gaaaacatca ttcatttctc agggaactta cgggattgga cagggctgcg cgaaggacct
     5281 ggaggccaac gatcagcaat acggcggtcg tgagcagtgc aagtcccgga acaaaccaaa
     5341 gcggcgacca ggtaacccgg taggcatagt tttgcagcca taaattgttg agcagggatg
     5401 ccagaggtat ggaaatcagg actgccagga ccaaccagcg gataaaagat cctgacaata
     5461 cccaaaacaa cttatccagc cctgcgccga ccactttacg gatcgcgatt tccttgcgcc
     5521 gcccggacag gatataggtg accatgccca gcaaacccat cagggtaatg gaggcggcca
     5581 gggccgcaaa aaacgcaatg atccacagga tatcccggag ggagcggtac gtcgctttga
     5641 cgtcatcggc ataaaacgac cactgagcag ggcggccggg atccagcgct gcccagctca
     5701 tgcgaatgga agccaccgcc tgctgcaggt ctgcggaccg gtccagggta agatggagga
     5761 tattgaactg ttcagggata taacgaagca ccaggggttc aatggcgtaa tcggccggct
     5821 taaaacggaa atcgcggatc actccggcta tggagaccag ggaggtatcc agaaaaatgc
     5881 ctttgccgac cacctccgaa ggatccccca tattgaaaaa acggaggaag gattcattga
     5941 ccagcacttg tttttcgtgt tgctggtcag ggtcgggttc gaagtttttc ccattgagca
     6001 aggtgaggcc aaaggcgggc agaaaatttt catccaccac ataatccctt actccaaggg
     6061 cgtccgcggt atccttggtt ttaacatcca ccttgctgtc ccggtaggtg cccatcagca
     6121 tggaagaggc ggctatggat ttgaccacgg gaagcttgga catttctgtt ttaaccctga
     6181 gatattcctg gccctgcagg tccaggttga ccatgtaatc ctgaccggcg ccaaaattgg
     6241 tgtcaagggc aaagcggatc tggagataag aagtggtgat taaaaagaaa aaaacaaggg
     6301 tcatggccag ttggccggtg atgattgctt tccgcaggga aacttgtggg aggatgcgga
     6361 gaccggataa cttttgtatg gaaggcagga tggcgacgcg ggatatcatc atggcgggga
     6421 gcaccccgat caggactcca accaccaggg caaaaaccag gtaccatcca tacagcgccc
     6481 aatccatccg gagggtaatg tccatcgaat tgagaaaatc gagccggctg aacaggggaa
     6541 tgagccattg aagaataagg atgcccaggg ccgtggaaag caaggctacc aggatggatt
     6601 ccacgataaa ctggctgaag atattaagac gtccggctcc gttgattttc ctgatcccga
     6661 tttcccttgc ccggttgacc gccctggcga gggaaatgtt ggcatgatta aatccggcgg
     6721 aggcgacgat gagtatgcct aaaatggata aaaaccaaag cagcaccgag ggcatgcctc
     6781 ttccaatgct gttggataaa agaggcccgg gggtaatctt gtccatcggc tgaagcttga
     6841 atgaataacc tgcatcccgg gattccagat ggaggccgga gtaacggttt tgagcgacct
     6901 cgttcaactc ggcttcgatc tttgacttgt ccgcctgatc ggtcaggcga taaaaatgcc
     6961 agccggcata ataatccagc cagttgtttt tatcaaagcc aaacgcatca gcaccggtag
     7021 cggcgctgag ggtttccacc gaggtcaggg catcaaactc caaatgggtc ttgccgggaa
     7081 attctttcag gaccccggcc acccggagcg cttgttggtg gcctccccaa accaggttct
     7141 gccccagggc attgccgtcc gggaatattt tgcgggccag gccggcagtg aggaaaatga
     7201 cattgggttg attgagatcc cctgcattcc cttcctccag gctgaaacca aaagtcttaa
     7261 aaaaagaagg gtgtgtgccc agtccttcaa actgaaacct ggattcaccg agtacgagtt
     7321 ccccgttaaa ccggttgatc aggggcaccc attccttgat tgaagaagac tgggcggcca
     7381 tgaaggaaga taacgcatag ggggagctgg cataagcttc tgtccctccc cccttgcgtt
     7441 gggcatatgt caagactcga taaattctat tgggttccgg atgaaaccgg tcataattat
     7501 aggcgtcccg gatcagcatg atcaccagga ggcaggcgga aatgctgatg cccaggccga
     7561 acacattgat tcccgaaaag aacctgaatt tccacaggtc ccgaattccg gtcagcaaat
     7621 agtttctaaa catgattagg cttacggtaa acggtatgcc ataatcaaat gtatttgaaa
     7681 ttcaataact tagattacgt ttcgttaaaa gtactgtccg gttttgatac aataactgtg
     7741 cgataaatcc aaaccattcc cttttatcct ggaagccttg taatccgaag accctgaagc
     7801 acgatacgca tataatattg gattttctgg atactcaaaa cattaattca cgaattatgc
     7861 attttacttt attttaaatt catgatctcc tgactatact cagatttatt cttgatccgg
     7921 atcataattt agcccgggag cgggaatatc tttgttcttt attttttatc ctcgtctata
     7981 aataaattta gtgtaaaggg taattgaaaa ccagaatcaa aagtgatggt agggttttaa
     8041 aaaagattgc agaggataaa aatgattata tccattaaga caaaggattg cctgaactgc
     8101 tacaggcaat cctttttttt aacaggctcc atccatttta tattgaaccc aaaccacgca
     8161 tgggattttc tattgcatga attaggttta aaggttctga ccacaagcaa ccgtttcttt
     8221 gacattcatc aggtttcacc atcctgccct tgatttacat ttgcaccctg gaataatgaa
     8281 gacggaaata ttatcctctt ccggtccgta tttcagtttt tttagtgtaa agggtaattg
     8341 aaagttaggt caaaagtgat ttatatttca ataaaatacc tttattgatg aatgatttgg
     8401 gttaaaacaa attaaaagga ttgcctggcc ggtaatcctt ttttatttaa acattccgcc
     8461 ctgctccctg ctccctgcca acttgctaac ttgactaatt ctcctgcccc tatccccttc
     8521 tgggaaccac agatattggc agttcaatta aaaatatcaa tccgcggtgc tcctggtttg
     8581 atgatcacag ggcgaataat agaaaggatg actctaaagg caagactaat ggtcacccct
     8641 tcaggcctgc ccggctagca ttatttgaga acaggcgttc aggcaggcct gcccgacttg
     8701 cattgatgga gaaacagtgg ttaggcgggc ccaacataag tcaatggaat ttgaagaaag
     8761 acggcgggag ggggctggtg ccaatttctc tgcggttaca tcgcgctccc ggcgcactct
     8821 gcggtgattg taaaaataaa ccatcgtccg cacaggtgat tgccaccagg cgacccactg
     8881 gctataccag gtgtcaacca ccgctgcgca cctcacaaat gcggatcacc gggcgattac
     8941 caggtcgaaa atctcccatg caattactaa ctaactgact aactgactaa ctgactaact
     9001 ggctaactta ctaacttcat accatgaaag taaaattctg gggatgccgc ggttcgctgc
     9061 ctgcctcggt gacccacgac aatattgaat acaaggtccg gtttgccctc gaaaaagcca
     9121 tccggcttgg agtccaatcc tcctccgccc tggatgaatt catccgggac ctgccttttc
     9181 atatccgttc cacctatggc aacaataccc cctgtgttga aatcctggga ggcaatgaga
     9241 tcgtcgtctg tgatgccggc tcgggtctgc gcgacttggg ccgttacctg gtctctcagc
     9301 cgttttcagg ccccagggtc gtaaacatcc tggtatccca cctccattgg gaccatatcc
     9361 agggttttcc attttttatt cccgcttaca tgcagggcac cgtgatccgc atctggggct
     9421 gccatgacgg cctggaatcc gcatttgtca accaacaaaa ccctccctgt tttcccgtct
     9481 tactcgaaga catgggggca aacatccgtt ttcacccatt ggatctccat ggccccaatc
     9541 aaatcggtgg ccttaaggta acagccattg aacaacccca tccgggactg acctacggct
     9601 attgttttga gcaggacggg aaaaaaatag tgtacagcac ggacattgaa catcaggacg
     9661 aagtagagga cccgatgaat ccgtacgtgc aattttaccg cgacgccgac atcctcatta
     9721 tggattgcca gttcaacctg gccgaccacc tgtacaccaa acaaaactgg ggccattcca
     9781 gcaaccttat cgccattgag ctgggagtca tatccaaagc aaagaccctt tgcctgtttc
     9841 atgcagaccc cttcctcgat gatcttcaac tcgacaaatt tttacaggac agccggagat
     9901 accaccagat atacgccccg gattcggaga tggaaatcct gctggcctat gatggcatgg
     9961 aggtggagtg ttgaattgct aaattgttga attgtggaat tgttgaattg ctgaagtgtc
    10021 aaattgtctg aggactcgaa gattcagaga tttcatggtt gaggttgttc ccctgaccat
    10081 tgaccattga ccattgacca ttgactattg actattgact attgactact gactactgat
    10141 actgactcct gactactaac tcctgactac tgattacttt ctcgccatcg cgatcatcgt
    10201 aatatcatcg gattgttctt cattgccggc aaaccggcta aggtcctgtc gcaaggcatc
    10261 caccagggtc cttgaatcgc cgccggatga tttatggtca atgaatttca tcagccggtc
    10321 ttcgccatat tgttcctgac ggatattcat ggcttccgtc accccgtcgg tatacaagag
    10381 caggcgttca tccggttcca gagtgatcct gccttcctgg taatgaaagg catccacggc
    10441 gcccaatacc ggtccatgga tgccttccag ttttttactt ccctgtttat tgcggacgat
    10501 gggtggcagg tgaccggcat tggaatagac cagtgagccg gttctgagat taagcttgcc
    10561 gaggaaatag ctgacaaaca tggatttttc attttccgcc tgcagatctt cgttgaccgc
    10621 ggacaggatg gcggcgggtg aatccatttt aatgccatgg gccttgacca gggtcttggt
    10681 caccgccata aataaggcgg ccgggactcc tttgccggat acatctccaa taccaaaaag
    10741 caggtgctcg tcgtccgtca taaaaaaatc gtagaggtct cctcccactt cccgggcagg
    10801 atcgagcagg gcgtgcatgt ccaacaggtt tccaggagga tagattttgg gcagcatgct
    10861 catctggatt tcctgtccca catccagttc cttcttcatc ctgaggttgt gggctgcgat
    10921 gatccggttg aggtcttcca gttcacggtt ttgtttttcg atcaggccat ttttttcggc
    10981 taatatcttt ttcgaccgtt tgacctggat gaaattgaga taaatcacaa aacatacggc
    11041 aagcagggag atcgaggcga gtccgaggga acgctgcacg gttttttgct ggcggatgat
    11101 ctctttatcc cggctgatga tggtactgtc gctctgcctt tgtttgcgca gcaagctgag
    11161 attaagttcc gccatggcca ggttggtgtc caggacctgg ttggtcgaat ccagcagttc
    11221 tttcgccagg gccaggtaga gcagttcttt ttgtttgttg atcagttcct gcgctttctt
    11281 tttttcctcc gcctcagcca gttccttcag gtaagtctgt tcatccaggg cattggtcat
    11341 cctccggatg tcgatccttt tcttctcctc cgaaaacgtc cggtactgat cgtaatactg
    11401 gcgggacaag gcgggatttc ccgctttttc atacgtttcg gaaagatagg aataacagcg
    11461 gagcaggttg tccagcatgc gttcccggtt gtttatctgc cgtgaaatat ccgctgcttc
    11521 ttccaggaaa cgaaccgatt cctcataccg gcgaaggctg ttgagcgaaa ccgcgctgtt
    11581 aatcagggcc tcaatgatgt tttctttgtc gccaaaggat cttcgggtgg cgaggacttt
    11641 ctgccattcc tcaaacgaac gccggtagtc ccgcaaatcc gcatataaaa gaccgaggtt
    11701 gccgcggatc atcgccgcac cgctttcatt ccccaggccc tggtttaatt ccagggaacg
    11761 ttcgtaatat tcaatggcct ttctcgtctg gttgtggtcc cagtatatcc gcgcgatggt
    11821 attgaggtag tgcgaggctt gtttcaggtt attccttgcc agttcattcc tgaagttttc
    11881 ttccgcaagg agtacctggg ccgaatcatt cctggacaag ggttgcacct gcgcaaagag
    11941 cacagcacaa aacaggcagc aaattaatgc tccccccggt tttatcccct tgatgccagg
    12001 cttcctcatc gtgcagaggt aaagaaagat accgaccggg tggattcatt acccaccttg
    12061 tccacggcac ggacccggat ttcgtatttt ttatccggcg ccaggttccc aatcggattg
    12121 gaataggcca actccggtcc gtcattgatc cggtagtaga ttttatcatt gcctacgatt
    12181 tcgtcggtag ccgccagaaa aaggactaca taagggggat ataccccgag tccatcccgc
    12241 tgggccgaag ggctggtgct gaaatttgaa atgataatgg gcgatcccga atcaaaatgc
    12301 accgtggcct cgctgacatt ccggttattc acctggtcgt aagcaaacaa ttctatccgg
    12361 tggacgccgg aaggcaggtc caggccaaac ggtttttcat accgggtttc cgcctgggcc
    12421 ccgtcgatgc tgtaagccat gtattggagg ccggactggt tgtccttgcc ctgcaacacg
    12481 aaggtggttt tgggacccat aaacaattca ttattcgccc ggaaggtggg accgttgaag
    12541 gaatggccga tgtcgggacc ggtgaggtcc aggaatacga ggttgatgtt gtgtttgtat
    12601 tcttcagccc cgcccggggt attgctcagg cggtcgatcg aataaaaccg gatggtgtga
    12661 ttgccctgga cggccggaag gtaaaaaggt tcttcatagg tcttgaacgc ttcctggtcg
    12721 acggaatacc ggatttcctt gacgccggcc ttattgtcga tggcagtgag tttcattttt
    12781 gttctcccgg aaaaataaac tttatcctgc cagatgtatt tatcccccag gatatccgaa
    12841 gccgtgatgg gagccagttt gtcgtaataa aaccggaaac cggcattgac gctgcggttc
    12901 ccgacatgat cttccgcata atattccagc acatggtccc cgtcttcgag gtgatccagt
    12961 ttcaggttgg ctccagtgta tatggaaaat ggagcctggt caaatcggta atggatcctg
    13021 gccactcccg acaaggggtc ggtggcggtg aaatagatgg tggacgtgga ggcaatcgta
    13081 ttgctgtcgg agatcccgtt gatattatgt gaaatgaccg gggccgaaag gtcggtggta
    13141 aaaaccgatt gttttactgg ttccacattg cccaccctgt ccgtggagta aaagcgaacg
    13201 gtttgttcgc cttctcccgg aactgaaatg gcctgctgat aaacgctgaa tgggtcgcta
    13261 ttgatggcag agtggataaa ttccacgccg gcgtatttat cgcttgaggt cagttcataa
    13321 gtcaggcccc ttccataata ttgttttcct gcggaagaaa acgcattggc atttttaaaa
    13381 cttatggtgc tttccggggc ttctccatca gcaaccactt caaacaggac ttccagctgg
    13441 ggttttaccg tttgacgggt atttttatcc accgcgtacc gggtccggat aaagttgggc
    13501 ccttcggtat ccaggaagac cggattcgta tatttctcaa attctttgct gaccaggttt
    13561 acgcccgccc catccgggga aggggccagt ttaatataaa ccggcaaagc cctgttccag
    13621 taaagggtgg aatccggacc gataaaatac cggggaaccg gcggtccggg ctgggcaacc
    13681 aacacctggc agcaaagcat caggaaactc ccggccagcc ctgcggggaa aatgttttga
    13741 atccgaattg atttcatgag tcaatatagt ttttagagta attcaattcc agggtcagtt
    13801 tgttttggtt cccctggcga aggtagtcca gccggtccat cagcctgccg accaatttca
    13861 gccccaggcc tccgatgcgg tccaggtcct ctgcggaccg cccttccgtg ttccggataa
    13921 attcaatagg gtcaaagggt tttccgtcgt ccagaaattc caggatcagc ttgtcttccg
    13981 tgcattccag gtttatgcgt ataaggtgac ggccctggtc ctggtaagca taggaaatga
    14041 tgttgctgag gatttcatca acggctatgc tcaccttttt acgatcgtat tccccgagcg
    14101 atttttccct gaggtatcgg tccagggacc ggtgcacccg gttgatttct ttgatttgat
    14161 tggtgatctc aatggtcatc cccttagttt actgaatcaa tggaacccct ggatggccga
    14221 agcctggtcc ggataaaatt taaagatcat gttgaagccg gagatatcaa acacttccag
    14281 cacggtccgg ttgagggaac aaaaccggat ctcgccgtcc atttttttca ccagcttatt
    14341 tgcagcgaga aagatgcgca ggcccgcgct ggagatataa ttgagttgtt ccagatccac
    14401 gatcaacttc cggtttccac ccatgatcag ttgaataagt ttagtctccg cttcgccggc
    14461 agtattggca ttgaggtccc cggacaggct gatgattttc acgtggtcca ggttttggat
    14521 ttcaaattcc atagtgaaaa ataaagaatt caaattcagg ttaaaataca atttttccgg
    14581 cggtgcgagg ccgtaaattt agaatcctgc agtgggcgcc ggccgaaaac ggacagaaaa
    14641 aggggaggtc ctgaaacaca tttttactac ttttagatga ccaagccatc atacctcaac
    14701 cgattattat gaaattattg ttgtcttccc tcctgctgtt cctctttata taccctggct
    14761 gtaaagctcc ggaaaaggaa acggccgccg ggcctgaccg gaaagccctt gcggataccc
    14821 tggaaaccct gctgaagcaa aaccttttgc acccctggta tcccgctgcg gtggattcgg
    14881 tgcacggggg ttacctgtcc accttcgact ccgctttcca tccggtcggg gaacagaata
    14941 aaatgatcgt ttcacagggc aggcatcttt ggaacaattc gagagcagca cttcattttc
    15001 cgaatgaccg gccctacaaa gcttatgccc gtcacggctt cgaattcctt cgtgataaaa
    15061 tgtgggatcc ccaatacgga ggattcttca ccttggtcga ccgcatgggt aatccccagg
    15121 gagaaggatc caagaccgcc tatggcaatt catttgccat ttatggcttg tccgcctatt
    15181 tccttgcctc cgcggattct ggcgccctgg atctcgccaa aaaagctttc ctctggctgg
    15241 aatcccatgc tcacgattcc gtgcacctcg gttacttcca gcatttatcc cgccaggggg
    15301 aaaggcaacc aaggcttccc actaccccat ccacttcaga cctgggctat aaagaccaaa
    15361 acagttccat ccatttgctg gaagccttta cggaattgta ccaggcctgg ccggaccccc
    15421 tggtcaaaac acgcctggat gaaatgcttt tccttatccg ggacaagatc gtcaatgaaa
    15481 agggcaacct agtcctgttt tttcagccgg actggacccc ggtgagtttc gccgattctt
    15541 cccgggaatc catcctgagg catcataacc tggaccacct gtcattcgga cacgatatcg
    15601 aaaccgctta ccttctgatg gaagcctccc atatttcagg ctgggaaaac gacagcgtga
    15661 cctgggccag ggccaaacaa atgacggatc aatgcctgag gtatggatgg gacgatacgg
    15721 tgggaggctt ttatgatgaa gggtataatt tcaaggatac tcccggcacg accatcatcc
    15781 gcgataccaa aacctggtgg gcccaggccg aagccctgaa taccctgctg atcatggcgg
    15841 acctttaccc ggatgatccg ctccaatatt tcgacaaatt tctcaaacaa tggtcctata
    15901 ttgaccggta tctgattgac cgtcaaaacg gagactggtt tgacagcggc ctggataaaa
    15961 acccggctgc cgcaggaagg aataaaggtc atatctggaa agccctctac catcagtacc
    16021 ggtcgctgga gaattgtgtg gtgaggctca ggggggatca atagtcaata gtcaatggtc
    16081 aatggtcaat ggtcaatggt caatggtcaa tggtcaatgg tcattagtga ggagtcagca
    16141 gacctgacca ctgactactg attactgatt actgactact gactattgac tactgactac
    16201 tgactactga ctattgacta ctgactattg actactgact actgactggc cgaactcaat
    16261 ccaaactccc ccagggcttt caccttctga aaaagcatat taaaatattt tcttctttcc
    16321 tttgcattgc tttgggtgag ggactcggaa gaattgacga tttttttgaa atactctttc
    16381 atatgggatg tcattctcgg cgactccgta tagatatagt tagggctgtc gatcatggta
    16441 tacacgcggc agccgtgatc ccactccacc ataaaattga ggctttgatt gagcatatca
    16501 ctcctgtaaa actggacgtc acccggattt ttagtgccgg aattggcgat ctcctccatt
    16561 aatgcgatca tggattcaag gtcccggatc agcaagcgga tctgatcgga atgcagaaac
    16621 ccctggactt ccgcgaggaa gcggatctga tagatcgtat tgtcaaaaag ggatgacggc
    16681 agatactcga tggacggcac ccgggaatac aagccgacaa tcttcttaca gatttcatcc
    16741 accccggaaa actctttatc ttccaggtca agcacggtgg cgctggtaaa acttttgagg
    16801 ttccaggtat aataggacca gatggccagc ttgagtttga taagcctggg ataagcgcag
    16861 gtcagaaaca acggaaaatc atgggacgta taatggattt tgggattgga caaggtcttg
    16921 agccactcca ggtaaaagac caggccttgt aaatattcga tcggggcgtt gattttccta
    16981 tcgatggttg cactccggta tatgatgcta tccgaatccg ggagcaggat ttcctggata
    17041 tcgatattat aggttttaca gatcaggata gcctcgttga tggtaaactg ggtatctcca
    17101 ttgagtcggt tgtaaatggc gcggttttgc aggtgcagca actcacccaa ttctttgacc
    17161 aggatgtttt gggcagggcc ctttaattta agcgccgtga tgaacttgtc gtaaaaatcc
    17221 ggtaaagggg ctgccataaa aagtaattta aagcgctatt tagactctgt ataagtaagt
    17281 tagaacacct ccaccctggc ttggcggtat tgaactatca atatacataa aggttttgaa
    17341 atgcccattc aggctggccg aatgaaaaag gtgtatgccg gaaaaaataa aaaacctcgg
    17401 gaagcaaaaa cgaatccagg attgcacaaa tcatgcacaa attcctgtct atgtagcctt
    17461 ttggtacaaa tagctttcaa acgggctggt tcctggaacg aatcagtcaa aattttccag
    17521 tttcaactat tgatatttga ttcattaaac ggaaacaaac catgaaaaaa tcaatccttc
    17581 ctaaccaacc ggcaagttgg acggttatac gatctctccg gtccgtctac tcttaccgga
    17641 aattcttgac aaatcgtaac ttttttcaac cgggagctac taataaagaa aataagctca
    17701 tgaacctgtt ccggaattta tcgatctgcc gctggatgac ctattgtatc cttgcctgcc
    17761 tggccaatcc aagggtccag gcccaggatc aactgagcat ctccctcttc aaccacgcca
    17821 ccacccttcc acccaaaggc ttctcaggcc ccttacaccc gggcatagat atcgggatct
    17881 ccacccacct gaagcccaaa gagaacaccc tttctttttt tcactggaaa ctgggttatt
    17941 attaccaccg cctggtacac cagggaatcc aactatatgg tgagtacaac aaacaatata
    18001 agatccttaa accaatcagc ctgggctggt ccggtggact gggctacctg cacacctttg
    18061 aattgcatga aaagtttatc ctgggagggg acggcacgta tcggcgcacc ggccgcctgg
    18121 ggaaacccca tgcccaggca tccctggccc tccaaatcct tggccggtgg ggatattggg
    18181 ctccctttgc cgaataccgg ctgaggctgg tcagtccatt tgtaaataaa tatgttcccc
    18241 tgctacccag cacctccctg cacttcggtg cttattttaa tttccacacc atctaataac
    18301 cggtactatg aaaaagctga tgattatcct gggcctttgc ctgtctgccc ttgcctgcca
    18361 gaaaggcgat attgatgcat ccacggcctg ttcattcacg tatcccccgg gcaatgacaa
    18421 ccatcctaaa gccgcaagac tccgggacat cctgaacaag tacaataaac tgggtcttcc
    18481 aggtatatcc ctgttggtcc gggatggctc aggcagctgg attggagctg ccgggatggc
    18541 ggatattgaa aagaatattc ccttccagcc ttgtaccgta gccaaggtgg ccagcatcac
    18601 caaaatgttt accggcaccc tcgtccacct cctggttgaa caacatacct ttaaactgga
    18661 cgataaggtg gatgccttcc tcgatgaaga agtactggac aaggtcaaaa actgccgcgg
    18721 agcgacgatc cgtcaactca tgaatcatac cactggcatt tacgatctga tcaccagcga
    18781 tgccttttac ctcgctttat taaacgatcc ggacaaaaaa tggtcagcgg aggaactcat
    18841 taaattcact tatggcaagg aaccgaaatt tgaattggga aaaagttgtt tttattccaa
    18901 tacgaatacc ctgctcctca gcatggtgat ccagaaagca acgggcagga caaatcagga
    18961 cctgctccgg gaaaaaatcc tgcatccact gggcatgaat gacacctact attattcgca
    19021 cgatgaactg cccccgatca cggcccaggg atattttgac ctttacaaca atcaacgcat
    19081 agtcaacgtc accaatttta ataccggaag tggaaatggc tacggaggat ttttctccac
    19141 ggtgctggac ctgcaaaaat ttatagaacc cctgttgcgc acaaaaacca ttctgaccgc
    19201 cggttccatg gtagaaatga cgaatttcat tccggaagct gatccggagg accccgccaa
    19261 cgatttaaac ctgggggccg gtttgatgaa aaggtatttt aatcaaccgc ttacttctga
    19321 caaatatggg tatggacaca ccgggcgcga cctgggatac agcgccaatg ctttttattt
    19381 tcccaattac gatgtcacct gttgttttat ggtcaattac ggaaccaatg cggaaagtga
    19441 attaagggaa gtgttttttg atttccagga tgagctgacc gatgctttgt tcaattaatc
    19501 cttataagat caaaccttat ttacctgcca caagaaatca atactttaga tgcaggaaat
    19561 gaattttcaa aatttccaat aacaataaat gcatccgcat gaagccgtta gcattgaaaa
    19621 accatttctt cattctcctt tggttaattc cgcaaatggt cttcgcacaa gttgaactga
    19681 atacaaccaa actaacttta ttggaaggta aatttgaaaa aggaagcaga atcattgaat
    19741 tcaaaccttc ctcacttata ataaaggtaa aaatgaaaaa tctaaatcga tttacagaac
    19801 aagaaattcc agcggaggcc attaaagaca ttactataaa atatgactta agccctgtca
    19861 ggggagcaat gagaggattg atgatcggcg ggggacttgg cctgctcatc ggatccggat
    19921 tgtattattc tggacctgta ggagcatttg tcgcctttgc tgctctaggg acaggaatcg
    19981 gactgggtgc aggtggcaaa aaaataaccc gatttccaat taatggaaac ctaaatgaat
    20041 accagaagaa tcttgatgaa atgcagagat tactgatatt aaaatgaatt tgcacttcct
    20101 gtactccggt atacccgcat cctagagggc gtgaatccac ctatccttta aaaattgact
    20161 gaaaaaaaag tcagcattca acagcttgaa tgctgactac tgactattga ctattgacta
    20221 ttgactactg actattgact attgctcaac tccacaacct gtcccaactc cggatattat
    20281 cccactccgg cgtcggcccg aagaaattgg tattttccgg tgccaggtgt tttttcatct
    20341 cttcttcatt gagttctata ccgaggcccg gggtatcggg aacggcagcg aagcccattt
    20401 cataaagggg cttgccgtcc accgttttga ccaggttttg ccaatatggg acatcgatcg
    20461 aatgattttc caggcccatg aaattcatgc ttgcggctgc gcagtgcacg ttggccatga
    20521 aggatatcgg cagtcctgca aagtgcatgg ctgaagcgac accgcgctcc tccgcatagt
    20581 ctgcaatctt tttcgtttca agcagtcccc cggaggtcgc caggtcggga tgcacgatgt
    20641 ccaccgattg cgtatcgatc aattttatga attcttcctt gaggtaaata tcttctcccg
    20701 tgcacaatgg cgtttcgatg gcggttttga tttctttgag ttgtgaagtc aattgccatg
    20761 gaatcatgtc ttccagccag gccagccggt atttttccac agccctgccc aggcggatcg
    20821 cgttgttgag gtcgaaatgg ccataatggt ctgaagccaa aggcacttca taacccacta
    20881 cttctctcac tttggccacg taattcgcca tttcctccag gcctttgtct gtaagttgga
    20941 cttgagtgag cggatgcaac atggctccat aactcatggg atcattggac cactggcggt
    21001 tgacatccca gaaattggca ttgacgatgg tcccggggat atcgcgtacc agttcgattc
    21061 caaagtccat tttgaggaaa gtaaagcctt tttctttcag cctgacccgc atcctttcag
    21121 caaaggcgac gggatccttg acctggggtg tatcggcata gagcctgact ttgtcgcggt
    21181 atttgccccc cagcagttga tacgcgggta cgttataggc ctttccagcg aggtcccaga
    21241 gggccatttc cacggcgcat acccccccgc cctggcgcgc gtgatagcca aattgtttga
    21301 tccgcttgaa gatctgttcc acactgcagg gattcatgcc tttgatcctg cttttcagga
    21361 aaagggcgta tctccagtcg gccccatcgc gcacttcacc caggcccacc agtccctggt
    21421 tggtctccag cttgatcacc gggcagcggc cgcctccgtt catgaccacg gcatatttca
    21481 tgtccgtaat cttcaggtcg gagggagcgg aaaagcggtt gacgcgcgag gttgcatgtt
    21541 caagcgtttc ttccggccgc gcaaacataa aactgcccag ggccagtccg cccagggact
    21601 tcttaagaaa tcccctgcgg tcattctgcg ttgctttttc agccggctgt tccataggga
    21661 gcgcatccgg ttcggtgtcc tttattccgt gtttttcgag aaatttttgt gaaggagtca
    21721 ttgtagtaat ttttagttag caagttagta agttggcaag ttagtaagtt agtttgacgt
    21781 tgaattgtat ggttttctta acactttaat gagcccattg atcattttgg aaatttccac
    21841 cgtcaataac aggagctgga ttgatttatg cttctccaaa taccccagtc tttcagccag
    21901 gtagagcatg gattttactt cgctgcagga agctcgggct actgtcaaga atttgatgaa
    21961 ttccttgttg gtatggcggt cgaagccctc tgcaatatta ttggagactg aaacggctgc
    22021 cctgtttatc tggcttctga aatcccagtc cttgatttcg tgcagcgtca aataaacttc
    22081 aacagcaaga tcctgaccct tctgccaagc cagaatgtct tcaaaacggg atattctcat
    22141 tagggttgta ttctcagata aaaatactgt gaagttctcc aatttcacaa attaggaact
    22201 aaaccccggc tagcctacga acttgccaac ttgccaactt gccaacttgc caacttgcta
    22261 acttgctaac ttgctaacta ctactctgtc ctcagtgact tcaccggatt catccacgcc
    22321 gccttcaccg cctggatggc taccgtaaac aaggcaaggg caacgaccag gaccatggcc
    22381 gcaatgaaca tccaccagga aatactggtg cggtgcacaa aatcctgcag ccattgattc
    22441 atcaggtacc aggtgagggg ggtagccagt acaaaggcga tgagcaccaa aaggacgaag
    22501 tctttgccga tcagcccgat gatgctccgg gttgaggcgc ccaggacttt gcggatcccg
    22561 atttctttga tccgccgcga aatggcaaag gaggcaaggg caaaaaggcc caggcaggag
    22621 atcaggatag ccaggacagc aaatcctttg cacaaggcgg caaactgggt atcggcctcg
    22681 taaaaacgct ggatctcttc atcgtaaaaa cggcctttaa agacttgctc gggaaagagg
    22741 ttgttgtaga ttttttcgat tgacgccacc gtctcctgga tgttctgcgg cctgattttt
    22801 actcctgcca cgaaataata ggccttgcgc gaagtcatca gcagggcttc gagcggttca
    22861 tgggcggagt ggctgtggta atcttcggcc acgccgacga ggttaagggt ccggttgccc
    22921 agcttgatca cattgcccag gaccgagttg aagtcggtga tgcctagttt tttgagcgcc
    22981 gtccggttga tcacagcttc ccttgccgta tcggatggca ggaaccacct gccggccagc
    23041 aatttaatgc ggtaagtctc ctggtaatcc tcatcacaaa atttggtgga gatgggaaaa
    23101 ggagcatctt ctttcccctg ttgcaaggcc cagtttgaag accaggtatt cccgctggaa
    23161 ggacggtcgc tgctgaggct caccaattcc gtaccgggca ggcctttgat ctggtttttt
    23221 agggtattca gtttggactg gctgacgctg tcattgctga agccgaacgt atagaccaga
    23281 tccttgttaa agcccaggtc catgtttcgg ataaaagaga gctggctgag cgcgatcacg
    23341 gtactgatga tcaggccgaa ggcgatgaca aactgcaaca cgaccagcac tttgcggagg
    23401 aaaacgccgg agccgatccc tttttcgaaa ttggcgcgga agatctggac cggttggaaa
    23461 gaggaaagga ctgcagcggg atagagcccg gccaagaagg tgaccgcgac aaacaataat
    23521 gaaagaaaaa caaacaggtt gcccccctgg aaaagggcga tatcggccgg cagggggctg
    23581 atgtattgca gccagggact ggtgagatat gccagcccgg tgcccagcaa aatagccagg
    23641 cccaccaaga tccctgtttc tgtcaggaaa ttagccataa gccggccatg cccgaccccc
    23701 agggtcttgc gaatccctac ttccttcgcc ctgaccacgg actgcgcatt ggacatattg
    23761 ataaaattga aacaggccat gaccaggatc aggacaccga tcatggaaag gatccatgtt
    23821 tttttcttgt ccataacatg gctgccagag gtgcctatat cctcattgta atgcaagtcg
    23881 gccaagggtt gggcaaaatg ttttttggcc acctgttcat ttttgtactc ctctttgccc
    23941 accgaggcga gcgcctggtt ggccgcttcc atctggtccc tggaatggag caaagcaaaa
    24001 agctggttgt tgctgctggt ggagccccat tccttgctgt aaaaatagaa ctccggatgg
    24061 gcctcaatcg tgggataact gataaaaaac actaccggaa agtcactggc ggcaggggcg
    24121 tcttctacta cgccgatgac cttcatggac ggctggagat tgtccagggt gatccactgg
    24181 cctactgcct gatccgttcc gccaaaacat ttttcggcca tggcccggct caggatgagg
    24241 tgattgggtt ggtccagcag ggcggattga tccggagtca gccatttcca gtcgaatatt
    24301 ttgaaaaaag aaggttcggt aaaaaaagcg atctggcctt cgtccacatc aaacttcttt
    24361 aagggcaagc cgctggacgg atcgggcacc gcgatcgtag accaggtctc cctaatcctg
    24421 gccgtggctt caaactgggg caccgtcctc ttgaccgcat ccatggccgg aatcggcaag
    24481 ccgggagtgt acccggtacc gtcttccttg ctgatctctt ccctcaccac tctcacaatc
    24541 cggtcgtaat ttgaaaattg tttattgtaa ctggcctcgt ggtccacgat ccggtaaatc
    24601 aggatcacgg cggcaatgcc aatggataaa ccgatgatgt tgatgccggt gtgcagtctg
    24661 tttttcagca ggttcctaac cgccagtttg aaattaaagg aagccatgga tttctgattt
    24721 ttggtaaata tttaagacct ctgcttgaat ccattcattc actgcatagg tcataccaaa
    24781 tttatttaat cattgatttt caaagagata gcaaatttcc ctcttgaggc actgtacgtt
    24841 tttgaacatt tcctgacatt tccggacatc tccgcatacc tggtcgatag caagcctgaa
    24901 accattatat ttgaaaaacc atgcccgtag cagagcctcc ccgcgaacgc accgattatg
    24961 aaatcgagag aaacaaaccc atgcccagtc ttaatcatgc cattatacag gggaatctcc
    25021 tttttcaact ccatctcaag gtccagaaaa agtattcctt cttgcccgaa gtcagccttt
    25081 cattaccagg catacccgtc gccgtacccg atatttctgt ttatccaaaa ctcactgccg
    25141 attttttaca cgatatcggg accatgaccg aaagcccctg gtccatcatc gaaatcattt
    25201 caccctctca atcaacggat gaagtccttt tgaaatttga gcgctgtttt aatgcggggg
    25261 tgcggtcttg ctggttagtc atccccggcc tgaaagccat cgaggtatat gcaaaaacag
    25321 accattaccc attttacacc tctgatatgc tccttcagga caaagagctg gatattgaac
    25381 ttccgatgca aaccatcttt gggttctgaa caggaagtca ttggattata aaatccattt
    25441 ttaaccccta aaaaagaaat ttcagtcagt cataatccag gtaattgaaa tacatttcac
    25501 gcccggtatg gattttatct tttgtcagcc tgtttaccga cacggccagc gagatgcttt
    25561 accccatcat gcccatctat ctgcaaacca taggattttc agtggtgctg atcgggatcc
    25621 tggagggcct tgcagaggct acggctgggc tgagcaaagg ttatttcggc agactttcgg
    25681 atctccgttc caggagggtt ccctttgtcc aggccggata tgccttcagt gccgtgtcca
    25741 aacccatgat ggctttgttc acttttccat ggtggatatt ccttgcccgg accctggatc
    25801 gtttgggtaa gggcatccgg accggggcca gggacgcgat cttgtccgac gaagcaagtc
    25861 cccggacgaa aggaaaagta ttcggattcc accgctccat ggatacgatt ggagctgtac
    25921 ttggccctgc cctggccttg ctctatttgt atttccaccc cggggattac aaaaccctgt
    25981 tcattcttgc cttcattccg gggttattgg ccgtcctggc caccctgctt ttaaaggacc
    26041 atacccacaa ggcggtcaga accccgataa gtacaaattt tttttcattc atgcggtatt
    26101 ggacagagag cccttcccag tacagaaaat tagtgaccgg cctgttgatc ttcgccttat
    26161 tcaacagttc ggacgttttc ctgatcctta aagccaaaca agccggactg gatgacagcc
    26221 tggtcatcgg cgtttatatt ttttacaacc tggtttatgc cctctttgcc ttccccatcg
    26281 gtttcctggc cgaccgggca ggtctcaaac ccgtctttat tggcgggctg gtcctgtttt
    26341 ccattgttta ttttggcatg gcgttcaata ccaacttcat tgtctttatt ggattgtttt
    26401 tcctttacgg catttatgcc tcggccaccg aaggaatatc caaagcctgg atctcgaaca
    26461 tttgtgataa aaaagacacc gctaccgcca tcggaacttt ttccggcttc cagagcattt
    26521 gcacgctgct ggccagttcg cttaccggga tgatctggtt tcaattcggg gccaccacag
    26581 ccttgctcac tacagcgatt gcaactgtga tcctgctcat ttactttaag acggtccctg
    26641 gcccggaaaa atcctgaata tatggtgact actgatcccc tgactactga ctactttatt
    26701 tctattatcg tgcctgcaat atgcctcaag cccgcgtcga tattaaaata atagcatacg
    26761 agcacacggt tattgtttag caaaacgctc caggtatatc caaggtccgt ccctcctcca
    26821 tcatccctca aaatgatctc cggagcagtg gcaaagtccg tgcattccgc attcaatatc
    26881 ctggtccgga tcccgaaagg cttatgacgg tatccatagg taatcaatat tctcttatcc
    26941 ggaaggggca gggcgtgcag gggatggccc ttgaacccca ttccttgcca gctaaaggat
    27001 ttgcccccgt tttttgacct ggctatgcag gcctggtcat cataattcgc agtgcgcagg
    27061 aaggccacaa gatcccctcc aggggtttcc aatacggagg cttcattaaa ctccgcctgt
    27121 tcatcttcgg ccaccaccga ggaatatttc caggacaagc ccttatctct tgaaaccaat
    27181 aaatgcgcag agccccggtt gtcctcattt cccgcagcca ccacccaaag aatgcggcca
    27241 tcctttgttt cccagagccc accccggtta taagccggca agggattgcc gaatatatcc
    27301 aatttgggat cgatgggtac ccgcatggga taataaggac ccttccactg atgtcctcca
    27361 tccgtggacc ggagaacata tccgccgaga aagatcgcct tgctaggtat tgaatagaac
    27421 accggttttt ttagtttatc atacgcctcc tgactgaccg gcctccagcc ataactggtg
    27481 cagatcaggc tgccgtccct taactgcagc aggcagggat cctgcgagcc gccaaagggg
    27541 tgggcataca acaaatccgg attcttcgtc catacttctc catcccttga acggatcgta
    27601 acgaggtaac tgtttggatc aatatgctca tagttttttt cacccatggc ctgcagattg
    27661 ggagcccttc ggaaagccag taggaattcc ctattgggtc tttttaccac agaggggaaa
    27721 gcggcataaa acaaagaatc ctgatagatc attatatctt ttattttacg tacgccgtgt
    27781 actgattccc cgggcttgag agcagattgg gcgacaaggc cttgaagagg aaatgcaatg
    27841 agaactccgg gaaaaaagaa ggtgcaaaga aaatgtttca tgtgtccatg ctttatttgt
    27901 aagcttcatc cacctggctg cgaagttccc ggtaagcagc cggtccctgt gtctccaggt
    27961 ccttccagcg gcactcgagt actattttcc cctggtaccc ttcctttctc aaggcggcga
    28021 aataagggac caaatcttct ccatgaacac caggtggcgt gcgccctact ttttcggcaa
    28081 cctcgcagta caccaggtag tcttttgtgc ctgtgatcgc ctcaggcgac tccccctcca
    28141 tcagcatgtg ataaatatcc acactcaggc ggaagttttt atgatctacc gctttcacca
    28201 cggccagcgc ttcagggatg gtgtttataa agttacattc cgtcctgttc aggctttcga
    28261 gggcaaggat gatattttgt ttttccgcca aggaagcgat ttttttagcc atgcggataa
    28321 attgttcctc cgcctcgctc cggttaaaac tcccgggcac cccgcgcgag ccaccgcttc
    28381 cccagatcag cattttgata ccggctgctt ctgcacgcct gactacctgg tccacatagg
    28441 tcaaaaccgc ttcttcatca atggtcggtc ccactacttt cagatcaccc gggatgaaaa
    28501 ggttgcaggc gtataaagga accctcaact ttttgatcga aaggagatgc tcctggaatt
    28561 gccggtcaga gacactcctt ggagaaagca ttttttgtgc ggattcaacc agataccgga
    28621 agccaaattt gtaaagcaag cggtcattct cgatattttg caccaccccg atttcaggcg
    28681 gggttaattt tttttgcccg aaaaggggta tcgcggtaaa tccgaacagg aataaaaggg
    28741 agaacttttt caaggtttta aagatacgct aaaacaaagc acctcccggt caacgaacct
    28801 gaaacaattt ttgatccgta ttctttaatt ccaccttcct tttaaaacat ccaatatttt
    28861 gaaaatccca agcactgact aactgactaa ccgactcact gactcactcc gtcctcaaac
    28921 ttttgaccgg attcatcagc gccgccttga tcgcctggaa actcaccgtg atcaaagcaa
    28981 tggccagggc caggaacccc gcaaggccaa aataccacca atgcatattc tcccggtagg
    29041 tatactgctt cagccagtca ttgagaaaat accaggctat gggaaaagcg atcagggcgg
    29101 agatcagcac caggatgatg aagtccctgg acagcatgct caccaggttg ccgacacctg
    29161 cccccaggac cttgcggatg ccgatctctt tggtccgttg ttcggccgtg aacgcagcaa
    29221 ggccgaataa acccaggcag gaaatgaaaa tggcgaggaa ggcgaaatat ttggacagct
    29281 tgcctatggt ttgttcagaa cggtaggttt tggcaatttc ctcatccgta aaagaatatt
    29341 tgaaggggaa agcgggattg aactgtttga agaccttttc catgctggca atggcttctt
    29401 tggtcttgcc tgcttcggtc cggacgatca cattgcccca caagccccaa ctgcctttga
    29461 attggcggag aatcagcggt tctataggca cgtggaggga attaaaatga tagtccttca
    29521 tcaggccgat gatctgtcct ttgtcgcccc aaaccgtgag ttctttgccc acgggatccg
    29581 tataaccgat cttccgggcg gcggcttcat tgaccaggaa attgagggtg tcggtactga
    29641 aggacgggtc gaagtcgcgg ccgtcggcca gttcaatccc catggtgctg atataatcca
    29701 gggtgaccgg attgtggctg aagagggtca actgggtggt atccttgccc ggccaatgca
    29761 ccccctgggt cgaactgccc acttccatcg ggctgctttg ggaggcgctc acccctttaa
    29821 tgccgggctg ccggagcaat tcctccttaa aagtgtaata gctctttttc aggtccccct
    29881 ccagcggcat gaagatcagg ttttccttac cgaaacccag gtttttattg tttatgaagg
    29941 agatctggcg gtagatcacg atcataccca ggatgaggaa gatggaaagg gcaaactgga
    30001 atactaccaa accttttctt aaaaaagccg cccccggatt aaatttcaac aaacccttca
    30061 atatggtgat cggcctcaac gccgacatga acatggccgg ataagaacca gaaaccaggc
    30121 cggtcaccag ggccaggccc aataacaggg cccagtagga tccgttggcg agcttgatgc
    30181 ccagttcttt ctgggtcagg gtattaaacg acggaagaaa caaaaacaca atacccaggg
    30241 cgaaggccag cgcgataaaa ctgaccagga tggattcact cagaaattgc tggaccagtt
    30301 gcgatttcct tgctccaact accttgcgca cgccgacctc ccgggcgcgt ttcagggaac
    30361 gggcggtggc caggttcata aaattgatgc aggcaatcag caggatgatg acggccacaa
    30421 tgccaaaaat ccgcacatat tcgatgcgcc cccccgtctg ttttgcagac ttccattccc
    30481 catagagata agactcaccg taattctgca ggaagagttc tacgttgctt tccttgtttt
    30541 tggtttttat ataatctttg atctttgcat tgaccttacc gagatccgcg ttttcagcga
    30601 gcaagacata acatctcggt ccgttgttgc cccattcctt tgcccagtcg ttgttcttga
    30661 accagcggtc ataagacatg aggaattcga aggtaaggga agaggcctca gggatttcat
    30721 ccaggacacc ggtgaccact acattatccg cattgtcgat ccggatgatt ttattcatgg
    30781 gatcctgacc cggaaagtat ttgtccgcca gtttttgtga gatcaccact ccatccggcc
    30841 ggtccagggc ggagttctta tcgcctgttt tcagatggaa ggaaaacatt tttaggaagt
    30901 ctttttgcac ataacggcct ttttccttgt ccaggatatt cccgacggta aacagcggct
    30961 cttcttccca gagcatctgg ctggcgtatt gaatttccgg cacgtctttt acaatatgct
    31021 cagccagtat ccctggtgtt gaagagaagg tggatatatc ccctgaataa tattggtttt
    31081 ccatgacccg gtaaagcctg gcatcatgct catgaaaccg gtcctttttc aattcatcat
    31141 tcagccacaa tacaatgacg aggctgcagg ccatgcccag ggcgaggccc aggatgttga
    31201 tcagagaaaa ggttttgttg gataacaaat tgcgccaggc aatgagaatg taatttctaa
    31261 acataaaact tggtttgggg ccgggaggcc ggtttcagta tagttacttc aagggagtat
    31321 aaaaagttac aatttaaatt atcgattgat aatgagcctt ttatggttta cactttttgc
    31381 agggtgttta acgagtgtgc ggaaatgata caggaggggt ggttggtgtc ccgcaactgc
    31441 ggggaggtga atggtgggag gtgaatagtg ggaggtggat ggtgagaggc attcgaattc
    31501 ctttattcag atcttaaata aagacaattc atctgatagt cagttgcatg cacaggaatt
    31561 aaacccaaat catgcaatag agtttcgtaa tgaaaaccgg aaggccaata aag
//
LOCUS       DAOQXT010000052        30496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1783_length_30496_cov_6.444171, whole genome shotgun sequence.
ACCESSION   DAOQXT010000052 DAOQXT010000000
VERSION     DAOQXT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 30496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30496
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1783_length_30496_cov_6.444171"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..805)
                     /locus_tag="PKM27_10465"
     CDS             complement(92..805)
                     /locus_tag="PKM27_10465"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNR07726.1"
                     /translation="MTRLSVNINKIALIRNSRGGNMPDLIQVARDCERFGAEGITIHP
                     RPDQRHIRYSDIQPLKEAVATELNIEGYPSQEFMDRVLSHRPDQVTLVPDPPGALTSD
                     HGWDTVLHSAFLEDVVKALKSKGIRTSLFVDANPRMVEGAARSGTDRIELYTGPYAHR
                     FPDDPEASVKPHREAALVAAECGLGLNAGHDLNLHNLKFYKDQVPGLLEVSIGHALIC
                     DALYMGLEKTIGLYLQELK"
     gene            887..1792
                     /locus_tag="PKM27_10470"
     CDS             887..1792
                     /locus_tag="PKM27_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNR07727.1"
                     /translation="MRILVLLALVLTGLFAGMILMIAVLMISGTSMQQILDGGYINSE
                     KVNLARLVLLINHLTMFLLPALAWAWIYYRKKWLSGLSLDRGLSGLQALAGIFFLIMA
                     YPIVSKSFEINQSWDLPAWMGEMEGQTAEMLKILLKMDHGGELLFNLLIIALIPGIGE
                     ELIFRGIIQQEIDRALKKPWLAVVFSSLIFSAIHFQFEGFLPRFLLGIILGLLLMWTR
                     NLWLPILVHAFNNGMQVVLAYFIPEMSDQELEESVPVTWLALVASVIVTALLGYWFHR
                     RRALPEIREEPEIAAPGASDQNDTV"
     gene            1789..2625
                     /locus_tag="PKM27_10475"
     CDS             1789..2625
                     /locus_tag="PKM27_10475"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNR07728.1"
                     /translation="MKDLPLRFLTAMVLGIVVIGSIYYSYLSFSLLLLLISVLSGYEY
                     LRLAAFGAEISNPEGIKRLGAALTGLPLALSILPGSSDVQGSAFIILMVTLLAMSFLF
                     SYFLFKNEAGDWRLYQAVCMALIYIGLPLLLFRWVAAGSGQYEWLKPMTLLLLIWCND
                     IGAYFTGRTFGKRKLYVLVSPNKTLEGFFGGLILTITCGLVLSIYVHPLSPVQWVFYA
                     FIISILSTIGDLFESLIKRRYGVKDSGSLLPGHGGFLDRFDAFIFVLPASGLFLYLFW
                     NS"
     gene            2649..3302
                     /locus_tag="PKM27_10480"
     CDS             2649..3302
                     /locus_tag="PKM27_10480"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNR07729.1"
                     /translation="MNIHKEGFIIIPVCALVFGLLATLVINFVHVLTVPVIILFSLLF
                     AWVLWFFRDPVRIIPLPGDDMVYAPCDGKVVVIENVFEPVFFKEQRLLVSIFMSPLNV
                     HVNRVPVAGQVKYNQYFPGKYLVAWHPKSSTENERNYSVIDQGQYQVGVMQIAGALAR
                     RIVNYLKTGEEVKQGQELGFIRFGSRVDLYLPLSAKVEVKIDELVKGNLTCIARMEV"
     gene            3303..4010
                     /locus_tag="PKM27_10485"
     CDS             3303..4010
                     /locus_tag="PKM27_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNR07730.1"
                     /translation="MNDPKPYRHLFFDLDHTLWDFDRNSKATLERVFEQNRLEELTSV
                     PFNEFHARYSIHNHFYWGQYGKGLIGQEELRWKRMHSTLLEFELDDPRLARHLSHTYL
                     DRLPESTLLFPHAREVLQYLKDKGYHLHILSNGFEEVQHKKLKYSGIQGFFEQVITSE
                     ASSFVKPDKEIFLFALRRSRAEASDSIMIGDNPEADIQGAANADLHSVYVDHLGKPSP
                     VLPTYTVRHLRDLEELF"
     gene            complement(4180..4806)
                     /locus_tag="PKM27_10490"
     CDS             complement(4180..4806)
                     /locus_tag="PKM27_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07731.1"
                     /translation="MKNLFLLAFALLILGASCKSRQEKEEANREEELAESIESSADKF
                     AEDIKNMVDPGGKEVRLVDLADLKALLPQDLDGMDRQDLTGEGVGVMGFKMNKAEARY
                     EKGDKSIKLSLIDFGGFSAALLGIAAWSLTDIYHEDDDGYEKSATWKGYKVFEKSNRK
                     DHTSSLALIFKNRLVLNAEADQVTIEKLKDIISDEILEDLEDLDLKEK"
     gene            4954..7398
                     /locus_tag="PKM27_10495"
     CDS             4954..7398
                     /locus_tag="PKM27_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR07732.1"
                     /translation="MIRILNHNHNLNLNLNLNLNLNLNLILILNLLFLLSFTSPAFTQ
                     NTLSPLTIEKIMRDPRWIGTSPTNIRWSPDSRFIYFHWNPGAGPVDSLYRYSLSESKL
                     EKVDERSAAQLVTADEISYTSDRSAWTWSQRGNVYYQSDSMAQPVKITHTLGREWNPR
                     FSFNDRKIVFTLDDNLYSWEIASGSLSQLTHFIKSAAPRESRPSQQEAWLKNDQLREF
                     EILRQRRERQDSLQSLNKRLEASDLIKPIYLGDKNLGGLQISPDGRFVFYVLSRVNRA
                     KATVVPSFVTESGFTDNLPARAKVGDPADPQELMILDLHRDSTYGVSVKNLSGIKDIP
                     TFIGEYLELMKSYQKDSAERALRFSNLQWSPEGRKAVVDIRAQDNKDRWICSLDPATG
                     RLSLLDRQHDEAWIGGPGIGGGFGGGNSGWLNEEEFWFQSERTGYSHLYKIHISSLAM
                     TPLTMGNFEILRATLSPDRNLFTLTTNEVHPGEQQVYHLPVQGGQALRITHKTGGHQA
                     FPSPDGKHLAFLYSYSNIPWELYVQENKPDAPPKQITDKASSPEFKTYSWRIPEGITF
                     TARDEAQVHARLYRPAKKTNNKAAVIFVHGAGYLQNAHRWWSSYFREYLFHNLLADQG
                     YTVLDMDYRGSAGYGRDWRTGIYRHMGGKDLTDHVDGARYLAEQHGVNPKKIGIYGGS
                     YGGFITLMGLFTTPDVFAAGAALRPVTDWAQYNHGYTSNILNEPFTDSLAYRRSSPFY
                     YAEGLKNPLLICHGMVDVNVHYQDAVKLAQRLIELKKENWELASYPMEDHGFVEPTSW
                     MDEYKRILNLFNKWLR"
     gene            7513..8106
                     /gene="nudK"
                     /locus_tag="PKM27_10500"
     CDS             7513..8106
                     /gene="nudK"
                     /locus_tag="PKM27_10500"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HNR07733.1"
                     /translation="MPNPHIEIIDSQILSDQWYVLKKITFRQEDRHGHWHTVSREAYD
                     RGNGAVILLYHKAKKTVVLTRQFRMPTYLNGNDSGMLIEACAGLLDHDEPEDCIRKET
                     EEETGFVVREVRKIFEAYMSPGSVTEVLHFFVAEYDDAMKKTQGGGLARDHELIEVLE
                     IPFDQSLGMIASGEIRDAKTILLLQYAALNNLFENDK"
     gene            8117..9541
                     /locus_tag="PKM27_10505"
     CDS             8117..9541
                     /locus_tag="PKM27_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HNR07734.1"
                     /translation="MSAGCRLLLSLFLFIFGVPLALLGEKESKTRVVVLTDIEADPDD
                     HQSLIRLLLYSNQLKVEGLIATTSIHQKNRVSPESIHEVLMAYQKVQPNLLRHESGFP
                     SYAYLKSKVKSGLAVYGMEGVGPGRDSEGSEWIIRVLEKPDPGPVWFSVWGGPNCLAQ
                     ALWKIRNTRKPEEAVKLYDKIRIYTISDQDDSGPWIRNEFKNLFFIVSPGYNYSNASW
                     TGITRVYPGSEAVTTNRQWLAEHIQQGHGPLGAAYPDVAYGMEGDTPAFLGLLPNGLN
                     DPEQPAFGGWGGRYELKIPVFRDSNSGPFRRENWPQDQPETRPIWTNAADSVYAPSEQ
                     KGYKSDQATIWRWRTEFQHDFAARMDWCFKPFHQANHPPRPALAHADRLTVKSGELFH
                     LNAKGTEDPDGDSMSFLWFQYKEAGTFDGYIPFRPYSPNLYDLPVKAPDVTSPQTVHF
                     ILKVTDKGSPALTRYKRVIVTINP"
     gene            9634..10212
                     /locus_tag="PKM27_10510"
     CDS             9634..10212
                     /locus_tag="PKM27_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNR07735.1"
                     /translation="MLTKVIRLSTLITLMNVPFTRYASAQKPQEAKAGTQTLYDFKLK
                     KLEDGSVLDLSRYKGKKVVLLNVASECGYTPQYADWQKFYEAYGKKTVVIGIPSNEFG
                     GQEPGSNQDISTFCQKNYGVTFPMAEKAVVKGEGKHPLYQWLTTKSLNGWNDKEPGWN
                     FCKYVINEKGQLTHFFGSKILPTDSEFLEAVK"
     gene            complement(10306..11505)
                     /locus_tag="PKM27_10515"
     CDS             complement(10306..11505)
                     /locus_tag="PKM27_10515"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNR07736.1"
                     /translation="MNPSIKLSLLLALVWIACGASRKGEGFPQGNSNPLPAAHSFQSY
                     IRELKGKKVGLVVNQTSMVGAVHLVDTLLKQGVRIEKIFSPEHGFRGEADAGEKVATT
                     TDPRTGILLVSLYGEKRKPAPEDLAGLDLLVFDIQDIGVRFYTYISTMTLLMEACAEN
                     NKPLIILDRPNPNGYFVDGPVLRPEFKSFIGMHPVPVVYGMSIGEYALMVNGEKWLSN
                     GAQCALKVIPCANYDHTMTYDLPVRPSPNIPSLRSTLLYPSICFFEGTNCSEGRGTEA
                     PFVIFGHPKYSKGDYQFTPVSRPGAKNPKLLHQLCNGHNLSNLSIEEIMSWKRINLKW
                     LIQFYADMKDKDPFFLANNFFNRLAGNDVLMAQIKAGKTEEEIRASWKADLDAFKKVR
                     EKYLLYE"
     gene            11558..12952
                     /locus_tag="PKM27_10520"
     CDS             11558..12952
                     /locus_tag="PKM27_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNR07737.1"
                     /translation="METKYAVIDIETTGGLPQRDRIIEIGIILYDGEKETGRFGSLID
                     PGISIPPQITRLTGITTEMVSGQPKFYEVAKEIVQFTQGAVFVAHNVRFDYNFIREEF
                     SRLGYTYTRKQLCTKRLARLSLPGLGSYSLESLIRHLDIQVKHRHRALDDAYAACAVM
                     DHVFRLEENREKAIELIHQGVKESRLPASISLDYLHSLPEACGIYYFHNRSGDVIYVG
                     KSKNIKDRVLQHFADHTQKAVHLQQQVDSITYEITGSELLALLRENEEIKKIKPEINR
                     ALRKKELPYGIYATVDEKGFIRFEVDKARPVNENTVLIKKFSTPANARAFLSAAVERY
                     GLCQKLTSLERSGGCCFHYQIGKCRGACQGLEPPESYNERALLLQEKLVFPQKTFLVT
                     ESGRSQGETTVLCFRQGICIGYQYKDEYSGSFLSEEDLIPLPADEDTPSILRNYFSKN
                     PRAVWKEWRVEAGN"
     gene            complement(13005..14444)
                     /locus_tag="PKM27_10525"
     CDS             complement(13005..14444)
                     /locus_tag="PKM27_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
                     /protein_id="HNR07738.1"
                     /translation="MFKLFRMGGLKNRSILWYRQDLRMHDNEALHEAARFGHQVIPVY
                     VFDARIFQGISRFGHPRMHPLRARFIVESVKALRQSWRDMGGDLIVRAGKPEEIIFSL
                     AGEFKTSMVYCNRERTRDEVRVQDKLERNLWSIGQEMRCTRGKMLYYTQDLPFPVPHT
                     PDHFPAFRKELERVIPVRPMLDIPDTTPVPIRPADAGEIPEPEDFRLGLERGLEPLFN
                     GGEKEGIRKLKEYIWSDGGLKRYRAKDNFLNNALFSSSLSPWLAQGCLSPKMVYHEVK
                     SYESIFGPGEASQNLLTELVRRDYCRLITNKYGDRVFQRGGILKKPVPHVRDETYLFN
                     LWKEGRTGVPLVDAAMKALRATGYLPHPLREAVGRFLVSSLKVNWQLGADWFEANLVD
                     YDAASNWVNWLFLAGQMPESGEERAVSLAYLAKKYDPSGHFIRKWLKELDPLPDSKIL
                     HPESLSKEEQVKYGFTPGKNYPRPIIPFG"
     gene            14477..14899
                     /locus_tag="PKM27_10530"
     CDS             14477..14899
                     /locus_tag="PKM27_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07739.1"
                     /translation="MNAKPLLIISLALLLFACASGGSGKEIAGEVRMLHDSLMKLHDE
                     TMASHSSCLSLIDELKDLSAAAPDAGKVQIDSVRTELDYTSEIMMDWMAAYQDPVTED
                     TVAAGYLKEQIKAVREIARIQELNLGAARVLLKKENPE"
     gene            14896..15510
                     /locus_tag="PKM27_10535"
     CDS             14896..15510
                     /locus_tag="PKM27_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNR07740.1"
                     /translation="MKIRILFLMVPVLWSCIHRPARLPILGEMDISGGDTVFHQIPDF
                     SFINQYGEEYNKDSIRGKIYVSDFFFTKCPTICPKMAQQLLRIHERFADDPDFRILSH
                     SIDPKYDTTEALRKYAEKLGVGQTRTWHFLSLPKDQINETARQYLAAVQEDSTAAGGF
                     THTGHFLLVDGERHIRSFCDGTKAEEVDRLMKEISILLDERRLQ"
     gene            15491..15865
                     /locus_tag="PKM27_10540"
     CDS             15491..15865
                     /locus_tag="PKM27_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNR07741.1"
                     /translation="MKGACNSLLFAFLLSAGLAACDFRSQGEVIYQHSCNYCHMEDGN
                     GLARLILPFEEHRFVEDRQILVCTVVKGLDQGDTGHFMPRFDKLDHAQLANVLNYIRG
                     LKKMQAPPFTDLEVSRLREACH"
     gene            15958..17898
                     /gene="nagB"
                     /locus_tag="PKM27_10545"
     CDS             15958..17898
                     /gene="nagB"
                     /locus_tag="PKM27_10545"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF002557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNR07742.1"
                     /translation="MSIQSTEEMITAASVTKFEKIPTQIYSSTKDGSAVVARQIADLI
                     RQKEKEGKPCVLGLATGSTPISLYNELIRLHREENLSFRNVISFNLDEYYPMQPDTLQ
                     SYVRFMHENLFNHIDIPAANIHIPDGRVPKAKIPEFCHDYENKIREAGGIDLQVLGIG
                     RTGHIGFNEPGAGINSVTRLISLDFMTILDAASEFFGEENVPKNAITMGVKTILGARK
                     IILMAWGEGKARIVQRAIEGDMDDSVPATYLQHHPDTTFILDQAAASGLTRIKTPWLV
                     DTCAWDESLIKKAVIWLSIKLGKPILKLTNRDYAEDGMGDIITEFGNAYNINISIFNK
                     LQHTITGWPGGKPNADDTNRPERAEPAQKRVVIFSPHPDDDVISMGGTFIRLVDQGHE
                     VHVAYQTSGNIAVFDEDVIRFADFIRDFHNTFGLDAKAAEAMYFDIKRQLENKKPGEV
                     DSRPVQTIKGLIRRAEAKAGCRYVGIPDENAHFLDLPFYETGLVKKKPIGPEDIRITK
                     ELLNKIKPHQIYAAGDLSDPHGTHRVCLQAVFQSLEELKKDKVDWIGDCWLWLYRGAW
                     QEWEIENIDMAVPISPEELMRKRKAIFKHQSQKDRPLFPGSDAREFWQRAEDRNRGTA
                     NAYNKLGLAEYEAIEAFKRYYF"
     gene            17902..18555
                     /locus_tag="PKM27_10550"
     CDS             17902..18555
                     /locus_tag="PKM27_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07743.1"
                     /translation="MIKIPVRLAWIAVCLLPMVLSAQVAIELTPGVKLTFPGPPEKLD
                     TLGQQVYTYSDENAYYSCIIQKTNIDPVKLASLDILQFYSEIYQTVQNPADQCRPISQ
                     KEVVIQDTRGMEFYAQCAEREDVPELRYKRLVLVPPNLYVIDFWTTRAASDQAAGRKN
                     AFFESMIFTPQGGQAVIPSALDKTESGGSGWTQYGYFLAAIAVLLVALLYLKKKPKK"
     gene            18630..20948
                     /locus_tag="PKM27_10555"
     CDS             18630..20948
                     /locus_tag="PKM27_10555"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNR07744.1"
                     /translation="MNKFLFWSVLGLVFISCQQSPREIVIAEDRKSNYRIIFAEEAPD
                     SLRTAIEILQNHLRLMTGADVKAYSDIVVPQAEEILVGKSKRMEKYLNKIPLADLGSG
                     YYEGVHDKKWIIMANSASAILSGIYDIVGRLGSVKVSQDLTQFTQVQRFTRSSDPYIF
                     KPTFSYRQAVTPLANNDQYRAFNQININNTDDWGTWAYSMERIFPATSYFSSKPQYFA
                     EIQGKRLPAQVNFSDPRMKEDLTKNLEVWTMSKGLASYWSIGPYSNRIVSEDELTRKA
                     IGETGSPSGALLQLVNEIAAENKSRTYAIWLDGPYRIAPDKIQPLNNVMVVLDTRDVD
                     HAVSLGEGQTNETFRRDLAAWKKQTSNIAVVSHLTNEEKFMMPFPNLYALQRSLKYLD
                     SEGVDKIIFVGNSFIGSAMPDLKFYVASNLAWNVDQPVDTLIKRYCDNKYGVAAASMY
                     AYVRALETTLQSTKSRLYVNGSPSDGFRSWLQPTNINQLYSYFSATTSVTGNNPDLRD
                     LVLLERLSLIYTQLEVAKSMGTNTYGYFMNIGALSQLIRQENTMRQEKATMDIRKKDW
                     GTIQGMRDLLDQFVNDCDRMSVRVVDAKGTTPAQYRTLVQKYIDQQIQTHFAFKRGTF
                     TFNVSPDPYFADGAETILYDGVAGLAENPQTNWMGVSGGAFEVTWTNDKDTILKSLTT
                     RFLQNTEVRAWLPTEVSCLVSADGTSFTEVNTVRFGPETLARAIRPVTFNLGNRKIKA
                     IKLKASGLKTCPPDNIQSGLPAALVVDELEIR"
     gene            21051..22640
                     /locus_tag="PKM27_10560"
     CDS             21051..22640
                     /locus_tag="PKM27_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNR07745.1"
                     /translation="MIDPKRPFRLTKKTRFYLHSLVRHDLKASITVFFVALPLCLGIS
                     LASNAPVYSGLIAGVIGGMVVTLISKSELSVSGPAAGLAALCAVAITDMGNIQYFFIS
                     VLLAGFFQFALGLLKLGGFTHFIPSAVIKGMLVAIGILLISKQVPLIIGYDKPDFWRG
                     ELFNIITFNHGFSHIENLYKTSSAGVVLLALLALVCLILWKKYASKKFEVLPTSFVTV
                     LFGVIISLLFAYWFPALALEPSHFVRVPQDIFSEVGWPDFSILFKSGEISSLIWKNAL
                     IFCFIASLETLLSVAAIDKLDPFNRITPPNRELMAQGTGNFLSGLLGGLPITAVIVRS
                     SANAEAGARSKASSFLHGFWILLAILLAIPLLNRIPYAVLAIILIRTGYNLAKPKMIR
                     EVYHQGREQFLPFIFTIVAILFTDLLIGVVIGITYAIYFLFKHTYHAGFQVKQSKEGH
                     SPKFTIELDQHVSFLNKRRLTETLDKIPEYAIVEINGTDSAYIDRDILEIVQDFRSKA
                     HRKHISLSTVQVPEVRTIELH"
     gene            complement(22988..24070)
                     /locus_tag="PKM27_10565"
     CDS             complement(22988..24070)
                     /locus_tag="PKM27_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNR07746.1"
                     /translation="MMKPEIPFNDLPFLPPPRAQIETIPVLRQVSHTAAAIGELKGLA
                     NTLPNPDILLNAVVLKEATASSEIENIITTQDKLYQALSIKRLNVDLSTKEVLRYREA
                     LLEGYRLLSKRGFLSTNDIVKIQQVLEENSAGIRRLPGTALKNAATGTTIYTPPDHQE
                     TILRLMKNLEDYLHSDDNLSPFIQLAVQHYQFESIHPFYDGNGRTGRILNVLFLIMKG
                     WLEKPVLYLSKFIIDHKSDYYRLLQEVRIRENWGEWVLFMTKAVEVSARETIEKIRQI
                     NNLFYRTLETVRKEKPKIYSKDLLELLFVQPYCRIEMVMEQLDVSRPTASRYLNELAG
                     AGILKTRQVWKENLFIHEALFNLLKG"
     gene            complement(24187..26676)
                     /gene="priA"
                     /locus_tag="PKM27_10570"
     CDS             complement(24187..26676)
                     /gene="priA"
                     /locus_tag="PKM27_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNR07747.1"
                     /translation="MPFFPDDLEQDQPGHNLFARVILPLNLDKPYSYHVPREFMETIR
                     PGIRVEVQFGRSKLYAGVVSALYHEAEIPPRYKSILSILDEAPVVTDLQLKTWEWVAN
                     YYCCGIGEVMAAALPAAYKLDSESKIVLHPDFDLDLSELDDQEYLIAKALSLHKAISI
                     EDMRKILQKKSINKIIDQLVTKGIILIEEELLSGLQDKKEEYLVLDPKFQGDPDRMVG
                     ALDLVAKSALQTQVMLAFIQYARPKQEILRKDLARHVNLTTPVLKAMEKKGIFILQKR
                     PAARGLPAELKIELPDMTADQSKASFEISAAFGDMKNVVLLQGVTGSGKTRVYSEFIE
                     KTVSQGGQVLYLLPEIALTTHLITRLKKALGPHLHLYHSRISQRERVDTWQAVLHGAP
                     LTLAARSGIFLPFTNLKLIVVDEEHDPSYKQYDPDPRYHARDLAIWLAVQCGAKVILG
                     SATPSIESWYHARQHKYGHALLEKRYQEVELPEIELVDLYRENQGKDPPDIISNTLHD
                     AILRSLRQEEQVILFQNRRGYAPQQHCSNCHWHAMCPNCDVSLTYHKYINQLVCHYCG
                     YRRALFSTCPDCEKPTVALRGFGTEKIEDELKQKYPDARVRRLDLDTARSKASYQELL
                     DQFEERNIDILVGTQMLSKGLDFDHVGLVGILQADSIFYYPDFRSNERAYQLLTQVAG
                     RAGRKERQGRVILQAYNLRHEVLKYIMNYDYTGLAEKELKDRQALGYPPFMRLIEITI
                     KHKKPQQVDQASLMLAKQLQSRFGERVIGPLLPSIERLRNYYLRNILIKMEKNSQVIQ
                     EIKSSLRTSIYEVLRQPGLSSTRFNIDVDPD"
     gene            26853..27566
                     /locus_tag="PKM27_10575"
     CDS             26853..27566
                     /locus_tag="PKM27_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07748.1"
                     /translation="MAQQPQYACVGRMSQGYVTKGSCVLIRPGVALTAAHTLTKLHPT
                     LPLMAVFDSMEVKVDSFRIHPWYYEFPEADLAILYLSVPLNHIPLPGLNRKKKETGQT
                     GTTVGFGNFSVANDPSHIIDAGKLKSAGQNMLDSVAGAPLPDKNRPQLFADFDHPEDP
                     RFNRSGTAECLPLEYGLDGGDSGGGLFIVHRGRTLLAGINAIQNKNIADIIRTRSFYG
                     SSSQWVRISVFRKWIRRNS"
     gene            27723..28703
                     /locus_tag="PKM27_10580"
     CDS             27723..28703
                     /locus_tag="PKM27_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNR07749.1"
                     /translation="MLFAFLKKVMTGTPIRKDSHFIHRLRLYLKSDWRKGFREYKIFT
                     LRGLKDFILISMGIFSAAFGLKGFLLTNQFIDGGATGISLLISNLTDIPLPLLILTIN
                     IPFVLLGFRVIGHTFAVKTALAILGLALVLGTVPYPDVTQDHLLVAIFGGFFLGAGIG
                     LSVRGGAVIDGTEVLAIYLSRKLSTTIGDIIILINVLIFSTAAYLLSVEIALYSLITY
                     LAASKTLDFFVEGLEEYIGVTIISTRSETIRNMIVNELGRGFTIYQGRRGFGKKGEIK
                     DIEIVYTVVTRLELNKLNSEILKIDDDAFIVMSNVKDTKGGMIKKRRLKN"
     gene            28731..30407
                     /locus_tag="PKM27_10585"
     CDS             28731..30407
                     /locus_tag="PKM27_10585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR07750.1"
                     /translation="MLRLYFPKRSTAALLLACNLFYSCKTSGPDADQIVVHARIWSDG
                     GIREYDALALKGDRILATGPTEEMMKYKSAATEVLDAGGRLLCPGFIDAHVHLLQAGF
                     SLSSVQLRDAGTPREFIARMAAFAKTVPAGQWITGGDWNHELWGGQLPSRHWIDSVTP
                     DHLVAVNRLDGHMILCNSLALERAGITGDVKEVAGGEIPRDERGEILGIFKDNAADLI
                     YAKIPAPEPALEDQALRKAMEYVASYGVTGVHHMGSFADLEVFRRNKDKLITRFYAAT
                     PLAQWADLKTYIDQYGRGDSILHWGMLKGFMDGSLGSHTAAMSEDFSDKAGDKGFFVN
                     PPDSMEAWILAADGEGLHIGVHAIGDRAISDLIDIYERVRVTHGPKDRRWRIEHFQHP
                     AAKDLTRMRELDVIASMQPYHAIDDGCWAEKVIGKERIQLTYAFRSLLDVKVSLAFGS
                     DWFVAPAIPAMGIYAAVTRQTLDGKNPGGWVPLQKISVDEALSAYTSGSAHAGFEDQY
                     TGSIRQGHLADLVLFEDDITTLPQDSIRTARVAWTMMGGRMVYQRDARNE"
BASE COUNT         7292 a         8220 c         7748 g         7236 t
ORIGIN      
        1 cgcttcccga agctaagacc ttcttttatc agatatcatg gaatctgact actgactatt
       61 gactattgac tactgactac tgaaccgact actacttcaa ttcctggaga taaagtccta
      121 ttgtcttttc cagtcccata tacaaggcat cgcagatcag ggcatgcccg atggatacct
      181 cgagcaatcc agggacctga tctttataaa acttcagatt gtggaggttg agatcatgac
      241 ccgcattgag ccccagaccg cattcggcgg ccacaagggc ggcttcgcgg tgcggcttta
      301 ccgaggcctc gggatcatcc ggaaaccggt gcgcgtaagg cccggtgtat aattcaatgc
      361 ggtcggtccc gctccgggcg gctccttcca ccatccttgg atttgcatcc acaaaaaggg
      421 aagtccggat ccctttggat tttaatgcct tcaccacatc ctccaggaag gccgaatgaa
      481 ggacggtatc ccatccgtga tcggaagtca atgcgccggg cggatcgggt acaagggtca
      541 cctggtccgg ccggtggctg agtacccggt ccatgaactc ctgggaggga taaccttcaa
      601 tattcagttc tgtcgccacg gcttctttca atggctggat atcgctgtag cgaatatggc
      661 gctggtccgg cctggggtga atcgtgatcc cttcggcacc aaaccgttca cagtcgcggg
      721 ccacctggat caggtctggc atattgcccc ctcttgaatt gcggatcagg gcgattttat
      781 tgatattgac gctgagtcgg gtcatggcgg ggacaggttt agaatctcat ttcagcccgg
      841 aacatcaacc cggggtttga aaaaccaaat atactatctt cgcccgatgc gaatcctcgt
      901 gttgttggcc ctggtgctta ccggcctgtt tgcaggcatg atcctcatga tcgcggtctt
      961 gatgatttcc gggaccagta tgcaacagat tctcgacggg ggttatatca atagtgaaaa
     1021 ggtcaacctt gcccgcttgg tcctcctgat caaccacctg accatgtttt tgttgcctgc
     1081 cctggcctgg gcctggatct attatcgcaa aaaatggctt tccgggctga gtctggaccg
     1141 gggtctcagc gggcttcagg ccctcgcagg gatcttcttc ctgatcatgg cctatcccat
     1201 cgtatccaaa tcctttgaga tcaaccagtc ctgggacctg ccggcctgga tgggggaaat
     1261 ggaaggacag acggccgaaa tgctgaaaat cctgttgaag atggaccatg gcggagagtt
     1321 gttattcaac ctgctgatca tagccctcat tccggggatt ggcgaagaat tgatcttccg
     1381 gggcatcatt cagcaggaga tcgaccgtgc cctgaaaaaa ccctggctgg ctgtggtttt
     1441 ttcctccctg atcttcagcg ccattcattt tcagtttgaa ggttttcttc ctcggtttct
     1501 cctgggcata atcctcggcc tcttgctcat gtggacccgc aatctatggc tgcccatcct
     1561 cgttcatgcc ttcaacaacg ggatgcaggt ggtgctcgcc tattttattc ctgagatgtc
     1621 agatcaggaa ctggaagaat cggtaccggt gacctggctg gccctggtgg catcggtcat
     1681 cgtgaccgca ctgctcggat attggtttca tcgccggagg gccctgcctg aaatccggga
     1741 agagcctgaa atcgccgcgc ccggagcatc ggatcaaaac gatacggtat gaaagacctt
     1801 cctctgcgtt tccttacagc catggttttg gggatagtag tcatcggcag catctattat
     1861 tcctacctca gcttcagctt gctcctgctt ttgatctccg tactctccgg gtatgaatac
     1921 ctgcggctgg cggctttcgg ggcggaaatc tcaaacccgg aagggattaa acgattgggg
     1981 gcggcgctca cgggattgcc cctggccctt tcaatacttc ccgggtcatc ggatgttcag
     2041 ggttccgcat tcattatttt gatggtcacc ttgttggcca tgagtttcct gttcagttat
     2101 tttttgttta aaaatgaagc cggggactgg aggctttacc aggcggtgtg catggcgctg
     2161 atctatatcg gactgcccct gttgttattc cggtgggtcg ctgccgggtc ggggcaatac
     2221 gagtggctca aacccatgac cctcctgctg ctgatctggt gcaacgacat cggcgcctat
     2281 tttaccgggc ggacttttgg aaaacgaaaa ttgtatgtcc tggtatcgcc caacaaaacc
     2341 ctggaaggat tttttggagg gctcattttg accatcacct gcggcctggt cctgtcgatt
     2401 tatgtgcatc cgctcagccc ggtgcaatgg gttttctatg cctttatcat atccatcctc
     2461 tccaccatcg gggacctgtt tgaatccctg atcaaaaggc gatatggagt caaggattcg
     2521 ggtagcctgc tcccggggca cgggggattc ctggaccgtt ttgacgcgtt tatctttgta
     2581 cttccggcct ccggcctctt tttatatttg ttctggaatt cctgaacgga ttccgtaaaa
     2641 acccaaccat gaatattcat aaagaaggat ttatcatcat tcccgtctgt gccctggttt
     2701 ttgggttgct ggcgaccctg gtgatcaatt ttgttcacgt gcttacggtg ccggtgatca
     2761 tccttttttc cctgcttttt gcctgggtgc tttggttttt cagggatccg gtgcgcatca
     2821 tccccctgcc cggggacgat atggtctacg caccctgtga cggcaaagtg gtggtcattg
     2881 aaaacgtatt cgagcctgtc tttttcaagg aacagcgcct gttggtatcc atcttcatgt
     2941 ccccgctcaa tgtgcacgtc aaccgcgtgc ctgttgccgg acaggttaaa tacaaccagt
     3001 attttccggg aaagtatttg gtggcctggc accccaaatc atcgaccgaa aatgaacgta
     3061 actacagcgt gatcgaccag ggccaatacc aggtcggcgt gatgcagatc gcgggagccc
     3121 tggcccggcg aatcgtcaat tatctcaaga ccggggagga agtgaaacag ggacaggaac
     3181 tgggattcat ccgtttcggt tcccgcgtgg acctctatct gccgctttcc gccaaggttg
     3241 aagtcaaaat cgatgaactg gtaaagggca atctcacctg catagcccga atggaagttt
     3301 aattgaacga tcccaaacca taccggcacc tcttctttga cctggaccat acgctctggg
     3361 attttgaccg aaattcgaag gccaccctgg aaagggtatt tgaacaaaac cggctggagg
     3421 agctcacctc cgtgcccttc aacgagtttc atgcccgtta ttccattcac aatcattttt
     3481 actggggcca gtacggcaaa ggcctgatcg gccaggaaga attgcggtgg aaaaggatgc
     3541 acagcaccct cctagaattt gagctggatg atccccgtct ggccaggcat ctcagccata
     3601 cctacctgga ccgtttgccg gaaagcaccc tgcttttccc gcatgcccgc gaagtcctgc
     3661 agtatttgaa agataaggga taccatctgc atatcctttc caatggattt gaagaagtcc
     3721 agcataaaaa actgaaatat tccgggatcc aaggcttttt cgagcaggtg atcacttccg
     3781 aagccagttc cttcgtaaaa ccggataagg aaatctttct ttttgcccta agacgttccc
     3841 gagccgaggc ttccgacagt attatgatcg gggacaatcc ggaagcggat atccaggggg
     3901 ccgccaatgc agacctgcac agtgtctatg tggaccacct ggggaaaccc agccccgtgc
     3961 tgccgactta tacggtgagg cacttgaggg atttagaaga gttattttga ggtccggggc
     4021 taaagcccaa tcttcaccct gctcctccaa ccccagccta aaggccgggg ttgattcact
     4081 tactaaggct tgatcgcaaa agttttttac agttaaaccc catggcgtta gcctgcggta
     4141 gtgaagatga gaaagagtct aggctttagc ccacttatct catttctcct tcaagtccag
     4201 gtcctctaaa tcctccagga tttcatccga aataatgtct ttgagttttt cgatcgtgac
     4261 ctggtctgct tcggcattca ggaccagacg gtttttaaaa atcagggcca gggaactggt
     4321 atggtctttc cggttggact tctcaaaaac cttgtatcct ttccaggtgg cggatttttc
     4381 atatccgtcg tcgtcctcgt ggtatatatc ggtgagggac caggcggcga tgccaaggag
     4441 cgcggcgcta aaacccccga aatcgatcag gctgagcttt atggatttat cccctttttc
     4501 gtaccgggct tcggctttgt tcattttgaa gcccatcacc ccgacgcctt ctccggtcag
     4561 gtcttgacgg tccatgccgt ccaggtcctg tgggaggagg gctttcagat cggccagatc
     4621 aaccagccgg acctccttac cccccgggtc caccatgttt ttaatatcct ccgcaaactt
     4681 atcggcgctg gattcaatgc tttcagccag ctcctcttcg cggttggctt cttccttttc
     4741 ctgcctggat ttacaggaag cgcccaggat caggagggca aaagctaata gaaacagatt
     4801 tttcatggtt tgtaatttat atttaagaaa actggctgcc gccccataaa attccatcca
     4861 tatgaaaata tcggatcaac ttcaactaga cgaagaccaa taacacctat tatcggaaaa
     4921 tattataatt ttcgttcaaa ccattgaata aaaatgatcc gtatcctcaa ccacaaccac
     4981 aacctcaacc tcaacctcaa cctcaacctt aaccttaacc ttaacctcat cctcatcctt
     5041 aacctcctct tcctcctatc ctttacttct cccgctttca cccaaaacac cctcagcccg
     5101 ctgaccatcg aaaagatcat gcgcgacccc aggtggatcg gaacctcccc caccaatatc
     5161 cgctggagtc ctgacagccg ttttatctat ttccactgga accccggcgc gggtcccgtg
     5221 gactctctgt accgctattc cctatcggaa tcgaagttgg aaaaagtgga tgaaagatcg
     5281 gcggcgcaat tagtcactgc ggatgaaatc agctatacca gtgatcgcag cgcctggacc
     5341 tggtcccaaa gaggcaatgt ctattaccag tccgattcga tggcccagcc ggtaaaaatc
     5401 acccataccc tcggccggga atggaatccc cgtttttcgt ttaatgaccg gaaaattgta
     5461 tttaccctgg atgacaattt gtatagctgg gagatcgcat ccggctcgct gagccaactt
     5521 acccatttta ttaaaagcgc cgctccgcgg gaatcgcggc ccagccagca ggaggcctgg
     5581 ttgaaaaatg accagttgcg ggagtttgag atcctccgcc aacgccggga aagacaggac
     5641 agtctgcaat ccttaaataa aaggcttgaa gcttctgatc tcatcaaacc catctacctc
     5701 ggagataaga acctgggcgg actccagatc agtcccgacg ggcgttttgt attttatgtg
     5761 ttgtccaggg tcaaccgggc caaggccacc gtggttccgt cttttgtcac ggaaagcggc
     5821 tttaccgaca accttcctgc ccgtgccaaa gtgggggatc ccgccgatcc acaggaactc
     5881 atgatcctgg acttgcaccg ggattccacc tatggggttt ccgtcaaaaa cctttcgggc
     5941 ataaaagata tcccaacttt tataggtgaa tacctggaac tgatgaagtc ctatcagaaa
     6001 gattccgcgg aaagggcttt gcgtttttca aaccttcagt ggtcgccgga gggccggaag
     6061 gccgtcgtgg atatccgggc ccaggataat aaagaccgct ggatttgcag ccttgaccca
     6121 gccacgggga gactgagcct gctggaccgt cagcatgacg aggcctggat cgggggtccc
     6181 ggcatcgggg gcggttttgg cggtggcaac tccggttggc tgaatgaaga agaattctgg
     6241 tttcaaagtg aacggaccgg ctattcccat ctttacaaaa tccatatttc cagcctggcc
     6301 atgaccccgc tcaccatggg caatttcgaa attctgagag cgaccttgtc ccccgaccga
     6361 aatcttttta cccttacgac caatgaagtt catcccggcg aacaacaggt ataccatctc
     6421 ccggtccagg gcggccaggc tctcaggatc acccataaga ccggaggaca ccaggctttt
     6481 ccttccccgg acggcaaaca tctggccttt ctctattcat acagcaacat tccctgggaa
     6541 ttgtacgtgc aggaaaacaa accagacgcc cccccgaaac aaatcacgga taaagcctcc
     6601 agccctgaat ttaaaaccta ttcctggcgc attcccgaag gaatcacctt cactgcgagg
     6661 gatgaggccc aagtccatgc ccggctctac cgaccggcca aaaaaacaaa taataaagcc
     6721 gctgtgatct tcgtacacgg agccggctat ctgcaaaacg cccatcgatg gtggtccagt
     6781 tatttccggg aatacctctt ccacaacctg cttgcggacc aggggtatac cgtgttggac
     6841 atggattatc gcggaagcgc aggatatggt cgcgactggc gcaccggtat ataccgccac
     6901 atgggaggca aggacctgac cgatcatgtg gacggggcca ggtatctcgc ggagcagcat
     6961 ggggtaaatc cgaaaaaaat cgggatttat ggaggctcct acggcggatt cattaccctg
     7021 atgggcctgt tcaccacccc tgacgttttt gcggcaggcg ccgccctccg ccctgtgacg
     7081 gactgggccc aatacaatca tggttatact tcaaacattc tcaacgaacc cttcacggac
     7141 agcctggcct accgcaggtc ttcgccgttt tattacgcgg aggggcttaa aaacccgctg
     7201 ctcatctgcc acgggatggt cgacgtcaat gtgcattacc aggatgcggt gaaactggcc
     7261 cagcgcctga tcgaactaaa aaaagaaaac tgggagcttg cttcctaccc catggaagac
     7321 cacggtttcg tggagccgac gagctggatg gatgagtata agaggattct caacttgttt
     7381 aataaatggt tgaggtgagt tactcagtta gccagtttgt cagttagcta gtttgtcagt
     7441 ttgaaagttt gaaaatccac tttctattcc caggcagcca accactaatc accatccacc
     7501 atccaccata aaatgccaaa ccctcatatc gaaatcatcg acagccaaat cctctcggac
     7561 caatggtatg tattgaaaaa aataaccttt cgccaggaag accggcatgg ccattggcat
     7621 acggtaagcc gggaagctta tgaccggggg aacggggcgg tgatcctgct ttaccataaa
     7681 gcaaaaaaaa ccgtggtcct gacccgccaa ttccggatgc ccacctattt aaacggcaat
     7741 gacagcggca tgctgatcga agcctgcgcc ggcctgctgg accatgacga accggaggac
     7801 tgcatccgca aggaaaccga agaagaaacc ggatttgtcg taagggaggt gcgcaagatc
     7861 tttgaagcct acatgtcgcc gggctcggtc accgaggtcc ttcatttttt tgtggcagaa
     7921 tacgacgatg ccatgaaaaa aacgcaaggg ggcgggcttg cccgggacca tgaactcatc
     7981 gaggtgcttg agatcccttt tgatcaaagc ctggggatga tcgcgagcgg tgaaatccgg
     8041 gatgcaaaaa ccattctgtt gctgcaatat gccgccctga ataatttgtt tgaaaacgat
     8101 aaataaatcc tctgtcatga gcgctggttg cagactttta ctttcacttt tccttttcat
     8161 tttcggtgtt cccctggccc tgcttggcga gaaggaatcc aaaacccggg tggtggtgct
     8221 taccgacatc gaagccgacc cggatgacca tcagtccctg atccgcctgt tattgtattc
     8281 caatcagctt aaagtggagg gactgatcgc cactacctcc attcatcaga aaaacagggt
     8341 ctctccggaa agcattcacg aagtcctgat ggcctaccaa aaagttcagc ccaacctgct
     8401 caggcatgaa agcggctttc cttcctatgc ctacctgaaa tcgaaggtaa agagcggctt
     8461 agccgtttac gggatggagg gcgtggggcc tggaagggat tcggaaggct cggaatggat
     8521 catccgggtg cttgaaaaac cggatcccgg tccggtttgg ttttcggtct ggggcgggcc
     8581 caattgcctg gcccaggccc tctggaagat ccgcaatacc cggaagccgg aagaagcggt
     8641 aaagttgtac gacaaaatca ggatctatac tatttccgac caggatgatt ccggtccctg
     8701 gatccggaat gaattcaaga acctgttttt tatcgtgagc ccgggataca attattcaaa
     8761 tgcaagctgg acaggcatta ccagggttta cccgggatcg gaggcggtca cgaccaaccg
     8821 gcaatggctt gcggaacata tccagcaggg ccatggtcca ttgggggcgg cttatccgga
     8881 tgtggcctat ggcatggagg gggacacccc cgcttttctg ggcctcctcc cgaatgggct
     8941 caacgacccg gaacagccgg ctttcggagg atggggaggg cggtatgagc tgaagatacc
     9001 ggtgttcagg gattccaact cgggcccttt tcggagagaa aactggcccc aggaccaacc
     9061 ggaaaccagg cccatctgga ccaatgcggc ggactcggtg tatgccccat ccgaacaaaa
     9121 agggtataaa agcgaccagg ctacgatctg gcgatggagg acggagtttc agcatgattt
     9181 cgcggcgcgg atggactggt gcttcaaacc ctttcaccag gccaatcacc ctccccgtcc
     9241 cgcactggcc cacgcggacc ggcttaccgt gaaatccggt gagcttttcc acctcaatgc
     9301 caaaggcacg gaagatccgg acggggattc catgagtttt ctgtggtttc agtataagga
     9361 agcgggcacc tttgacgggt atatcccttt caggccgtat tccccgaacc tctatgatct
     9421 tccggtaaaa gcccctgacg tgaccagtcc acaaacggtc cattttatac tcaaggtcac
     9481 cgataaaggc agcccggccc tgacccgcta taaacgggtg attgtgacca ttaatccctg
     9541 aaaccggtaa accattttcg tccgggtttg tttattgatt tattttagcc gatgaagtat
     9601 ttcctgctga tcatagccct cttattaatc cttatgttga caaaagttat ccgcctgagc
     9661 accctgatca ccctgatgaa tgtgcctttc acccgctacg cttccgccca aaagccccag
     9721 gaggcaaagg caggtaccca gaccttgtat gattttaaac taaaaaaact ggaagacggg
     9781 tccgtgctcg acttgtcccg gtacaaaggc aaaaaagtag tcctgctcaa tgtggcttcc
     9841 gaatgcggct acacccccca atatgccgat tggcaaaaat tttatgaagc ttacggcaaa
     9901 aaaacagtgg tgatcggcat tccctccaat gaattcggag ggcaggagcc gggttcaaac
     9961 caggacattt ccaccttttg ccagaaaaat tacggggtca ccttccctat ggctgagaag
    10021 gccgtggtga aaggcgaagg caaacatccg ttgtatcaat ggcttaccac caaatccctc
    10081 aacggatgga acgacaagga acccggttgg aatttctgca aatacgtcat caacgaaaaa
    10141 ggccagctca cccatttttt cggttccaag atcttaccca cggattccga attccttgaa
    10201 gcggtgaaat gagactcgag tggcgagcaa cgagtggcga gcaacgagtt gcgagtagcg
    10261 agcaacgagc agccagctta cccaacacca cccaccaacc accaatcact catacaaaag
    10321 atacttctcc ctcaccttct taaaggcatc caggtccgct ttccagcttg ccctgatttc
    10381 ttcctccgtt tttccggctt tgatctgggc catgaggaca tcgttgcccg cgagccggtt
    10441 gaaaaaatta ttggcgagga aaaaagggtc cttgtctttc atatccgcat agaattggat
    10501 cagccatttc aggttgatcc ttttccagga catgatctcc tcaatggaaa ggttgctgag
    10561 gttgtgcccg ttacaaagct ggtggagcag cttgggattt tttgcgcctg gcctgcttac
    10621 cggggtgaac tggtagtctc ctttgctgta tttggggtgg ccaaaaatga caaatggcgc
    10681 ttccgtaccc ctcccttcgc tgcaattggt cccctcgaaa aaacagatgg aaggatacag
    10741 cagggtggac ctgagcgagg ggatattggg agaaggccgg accggcaagt cataagtcat
    10801 ggtatggtca taatttgcgc aggggatgac ttttaaagcg cactgcgccc cgttggacaa
    10861 ccatttttcc ccgttcacca tgagggcata ttccccgatg gacatgccat agaccacggg
    10921 tacgggatgc atgccgataa atgatttgaa ttccggccgg agcaccgggc catcgacaaa
    10981 ataaccattg ggattgggcc ggtccagaat gatcaggggt ttgttgtttt ccgcgcaggc
    11041 ttccatgagc agggtcatcg tggagatata agtatagaag cgcacgccga tgtcctggat
    11101 atcaaaaaca agaaggtcaa ggccggccag gtcttccgga gctggttttc ttttctcccc
    11161 atagagcgag accaggagaa ttccggtccg ggggtccgtc gtggtggcca ctttttctcc
    11221 cgcatccgct tcgccccgga atccatgttc cggtgagaaa attttttcaa tccggactcc
    11281 ctgcttcaat agcgtatcga cgaggtggac ggcgcccacc atggaagtct ggttgactac
    11341 caggcccact tttttgcctt ttaattcccg gatataggat tgaaaggaat gggcggcagg
    11401 caggggattc gaatttcctt gcggaaaccc ctctcctttc ctggaagcgc cgcaggcaat
    11461 ccagaccagg gccagcaaga ggcttaattt tatggatgga ttcatctggt tgtattacgt
    11521 taattttgaa tgcgtaatat aatgccttaa ttctaaaatg gagacgaaat acgcggtcat
    11581 tgatattgaa accaccggag gcttgcccca acgggaccgc atcatcgaga tcggcatcat
    11641 cctttatgac ggggaaaaag aaaccggccg gttcgggagc ctgatcgacc ccggcatttc
    11701 cattcctccc cagatcaccc ggttgacggg aatcaccacc gaaatggtat ccggccagcc
    11761 caaattttac gaggtcgcga aagaaatcgt tcagttcacc cagggggcgg tctttgtagc
    11821 ccataacgtc cgttttgatt acaattttat ccgggaagaa ttttcccgcc tggggtatac
    11881 ctatacccgc aaacagttgt gcacaaaaag actggccagg ctcagcctcc ccggcctcgg
    11941 atcctacagt ctcgaatcgt tgatccgcca cctggatatc caggtgaaac acagacaccg
    12001 ggcgctcgat gacgcctatg ccgcctgtgc cgtgatggat catgttttcc gcctggaaga
    12061 aaaccgcgaa aaagccatcg aactcatcca ccagggtgta aaggaatccc gactccccgc
    12121 ctccatcagc ctggactacc tgcacagcct gcccgaagcc tgcggaatct attatttcca
    12181 caaccgcagc ggcgacgtga tctatgtggg taaaagcaaa aacatcaagg accgggtcct
    12241 tcagcatttt gccgaccata cccagaaagc ggtccacctt caacaacagg tggacagcat
    12301 cacttacgag atcacgggca gcgaacttct ggccctcctg agggaaaatg aagaaataaa
    12361 aaaaataaaa cctgaaatca accgggcgct ccggaaaaaa gaacttccat acggcatcta
    12421 tgccacggtc gatgaaaaag gctttatccg ttttgaagtc gacaaagcca ggcctgtaaa
    12481 tgaaaacacg gttttgataa aaaaattcag caccccggcc aatgccaggg cctttctttc
    12541 cgccgcggtg gaaaggtatg ggctctgtca aaaactgacc agccttgaac gttccggggg
    12601 ctgttgtttc cattaccaga tcggtaaatg ccgtggggcc tgccagggcc tggaaccgcc
    12661 ggaatcctat aatgagaggg ccctgctgct gcaggaaaaa ctggtctttc cccaaaaaac
    12721 attcctggtc accgaatccg gacgaagcca gggggaaacg acggttttat gttttcgcca
    12781 gggcatctgt atcggttacc agtataagga tgaatacagc gggtctttcc tgagtgaaga
    12841 ggacctgatc cccttgccgg ccgatgagga cactccctcg atcctgagga attatttttc
    12901 caaaaatccg agggcggtgt ggaaggagtg gagggtggag gctggaaatt agaagctcga
    12961 agctcgaagc tggaagctcg tagctggtat ctcgtatctc gtatctaacc aaacggaatg
    13021 atcggccttg gatagttttt acccggggta aatccatact tcacctgctc ctctttgctc
    13081 agggattccg ggtggagtat ttttgaatcg ggaagaggat ccagttcttt tagccatttt
    13141 cggataaaat gaccggaagg gtcgtatttt tttgcgaggt aggccaggct gactgccctt
    13201 tcttccccgg attccggcat ctggcccgca aggaataacc agttgaccca attgctggcg
    13261 gcgtcgtaat ccaccaggtt agcttcaaac cagtcggctc ccagttgcca gttgaccttc
    13321 agggaggaaa ccaggaaacg gcctaccgct tcgcgcaggg gatggggcag gtagcctgtg
    13381 gccctgagcg ctttcatggc cgcatccacc agaggaacgc cggtccggcc ctctttccat
    13441 aaattaaaaa gataggtttc atcccgaacg tgaggcaccg gctttttcaa gatgcctccg
    13501 cgctggaata cccggtcccc gtatttattg gtgatcaggc ggcagtaatc cctgcggacc
    13561 agttcggtca acaggttttg cgaagcttcc ccggggccga aaatcgattc gtaggatttt
    13621 acttcatgat aaaccatttt cggcgaaagg catccctggg ccagccaggg cgaaagggaa
    13681 gaggaaaaca aggcattgtt taaaaaattg tctttcgccc ggtatcgttt gagtccgcca
    13741 tcggaccaga tatattcctt cagtttacgg atcccttcct tttccccgcc gttgaacaaa
    13801 ggttccaggc cccgttccag gcccagtctg aaatcttccg gttcagggat ttcacccgca
    13861 tcggcaggac ggattggaac aggggtggta tccgggatat caagcattgg cctcaccggg
    13921 atgacccgtt ccaattcctt ccggaaggcc ggaaaatgat cgggagtatg cgggacgggg
    13981 aagggcaggt cctgggtata ataaagcatt ttgccccggg tgcagcgcat ttcctggcct
    14041 atgctccaga gattgcgctc cagtttgtcc tggacccgga cttcgtcgcg ggtcctttcc
    14101 cggttgcaat aaaccatgga tgttttgaat tcccctgcga gggaaaaaat gatttcctcc
    14161 ggtttgcccg cccgtacaat caggtcccca cccatatcgc gccaggactg ccgcagggct
    14221 ttgaccgatt cgacgataaa gcgggccctc agcggatgca tccttgggtg gccaaaccgg
    14281 gaaatcccct gaaaaatcct ggcatcaaaa acatagacgg gaatcacctg atgtccaaaa
    14341 cgggcggctt catgaagcgc ttcgttgtcg tgcatcctca ggtcctggcg gtaccagagg
    14401 atagaccggt ttttcaggcc gcccatccga aagagtttaa acatccagcc attatctttg
    14461 caaaaatata cggttgatga atgcaaaacc attattgatt atttccctgg ccctgctcct
    14521 gtttgcctgc gcctccggcg gatccggaaa agaaatagcc ggcgaggtca ggatgctcca
    14581 cgattctctc atgaaactgc atgacgaaac catggccagt cacagcagct gcctcagcct
    14641 gatcgacgaa ttgaaagacc tcagcgcggc ggcccctgat gcgggaaaag tacagattga
    14701 ttccgtccgc accgaattgg actacacgag cgagatcatg atggattgga tggccgctta
    14761 ccaggacccg gttacggaag atactgtcgc tgccggctat ttaaaggaac aaatcaaagc
    14821 cgtccgggaa atcgcccgaa tccaggaact caacctgggt gccgcccggg tcttgttgaa
    14881 gaaagaaaac cccgaatgaa gatccggata ctattcctga tggtcccggt gctttggtcc
    14941 tgcatccacc ggcccgcccg gttgcccatc ctaggggaga tggacatttc cggtggcgat
    15001 acggtttttc accagatccc cgatttttcc ttcatcaatc agtatggaga agaatacaac
    15061 aaggattcga tccgcggaaa gatttacgtg agtgattttt tcttcaccaa atgtccgacc
    15121 atctgtccca aaatggccca gcaattgctc cgtattcatg agcggttcgc ggatgatccg
    15181 gatttcagga tcctttccca ttccattgac cccaaatacg acaccacgga agccctgcgg
    15241 aaatatgcag aaaagctggg ggtcggtcaa accagaacct ggcattttct gtcccttccc
    15301 aaggaccaga tcaatgagac cgcccggcaa tacctggccg ctgtgcagga agactctacg
    15361 gctgccggag gatttaccca taccgggcat ttccttttgg tggacggcga gcgccacatc
    15421 cgttcatttt gcgatggcac caaagcggaa gaggtggacc ggctgatgaa agaaatctca
    15481 atcctgctgg atgaaaggcg cctgcaatag ccttttattc gcgtttctgc tttctgccgg
    15541 cttagcggcc tgcgacttca ggtcgcaggg ggaagtcatc tatcaacatt cctgcaacta
    15601 ttgtcatatg gaggatggca acgggctggc ccggctgatc cttccatttg aagagcatcg
    15661 ttttgttgag gaccggcaaa tcctggtctg taccgtggtc aaaggcctgg atcaaggcga
    15721 caccggccat tttatgcccc gatttgataa actggaccat gcccagcttg ccaatgtact
    15781 gaactacatc cgcggtctga agaagatgca agctcctccc ttcaccgacc tggaggtgtc
    15841 gaggctgcgg gaggcttgcc attaaagcgg tcccgaagtg cagcccggtg ctcctgctgc
    15901 cctgaacgca ttatttattt tcttaatttc acagacaccc aggcagtgat tacttttgtg
    15961 tcgattcaaa gtacagaaga gatgattacc gctgcctccg tgaccaaatt tgaaaagatc
    16021 cccacacaga tctactccag caccaaggat ggatccgccg tcgtggcccg tcagatcgcc
    16081 gaccttatcc gtcaaaagga aaaagaaggc aaaccctgtg tcctgggcct ggccaccggc
    16141 tcgaccccga tctccctata caacgaactg atccgcctcc accgggaaga gaacctgagt
    16201 ttcaggaacg tgatctcttt taacctggat gaatattatc ccatgcaacc cgataccctc
    16261 cagagttatg tgaggtttat gcatgaaaat ttattcaacc acatcgatat tcccgccgcc
    16321 aacatccata ttcccgatgg ccgggtccct aaagcaaaaa ttcccgaatt ctgccatgac
    16381 tacgaaaata aaatcaggga agccgggggg atagacctcc aggtcctggg catcgggcgt
    16441 acgggtcata tcggcttcaa tgaacccggg gctggcatca attcggtcac ccggctgatc
    16501 tcccttgatt ttatgaccat cctcgacgcc gccagcgaat ttttcgggga agaaaacgtg
    16561 ccgaagaatg cgatcaccat gggggtaaaa accatcctcg gcgcccggaa aatcatcctg
    16621 atggcctggg gggagggaaa agccaggatc gtgcagcgag cgatcgaagg cgacatggac
    16681 gacagcgttc ctgccaccta ccttcagcat catccggata cgaccttcat ccttgaccag
    16741 gcggccgctt cggggcttac ccggatcaaa accccctggc tggtggatac ctgcgcctgg
    16801 gacgagagcc tgatcaaaaa agcggtcatc tggttgtcta tcaaactcgg aaagcccatc
    16861 ctgaaactca ccaaccggga ctatgccgaa gacggcatgg gggacatcat caccgaattc
    16921 ggaaatgcct acaacatcaa tatcagcatc ttcaacaagc tgcagcatac cattaccgga
    16981 tggcctggag gcaagcccaa tgcggacgat accaaccggc cggaacgtgc ggagcccgcg
    17041 cagaagcggg tggtcatctt cagcccccac ccggacgacg acgtgatttc catgggaggc
    17101 acctttatcc gcctcgtcga ccaggggcat gaagtgcacg tcgcctacca gaccagcggc
    17161 aacatcgccg tatttgatga agacgtgatc cggtttgccg attttatccg ggacttccac
    17221 aataccttcg gactggacgc caaagcggcc gaggccatgt acttcgacat caaacggcaa
    17281 ctggaaaata aaaaaccggg tgaagtcgat tcacggcccg tccagaccat caaaggcctg
    17341 atccgcaggg cggaagccaa ggcgggttgc cggtacgtcg gcatcccgga tgaaaatgcc
    17401 cattttctgg accttccttt ttacgagacc gggctggtga agaaaaaacc gatcgggccg
    17461 gaagacatcc gcatcaccaa agaactgctc aacaaaataa aaccgcacca gatctatgcc
    17521 gccggagacc tcagcgatcc gcacggcact caccgagtct gcctgcaggc ggtattccaa
    17581 tcccttgaag aacttaaaaa agacaaggtc gactggatcg gagattgctg gctatggctt
    17641 tatcgcggag cctggcagga atgggaaatt gaaaacattg atatggctgt tcctatttct
    17701 ccggaagaac tcatgcgcaa aaggaaagcg atattcaaac accagtccca gaaagaccgg
    17761 cccctgttcc cgggttcgga tgcccgggaa ttctggcagc gcgccgaaga ccgcaaccgg
    17821 ggcacggcca atgcctataa taaactaggt cttgctgaat acgaagccat tgaggccttc
    17881 aaaagatatt atttctagac catgataaaa atcccggtac gcttggcatg gattgcagtt
    17941 tgcctgcttc ccatggtcct ttcagcccag gtcgccatcg aacttacccc cggagtaaaa
    18001 ctcactttcc cgggaccccc ggaaaaactg gataccttgg gtcagcaggt ctatacctat
    18061 tccgatgaaa acgcctacta ttcctgcatt atccagaaaa caaacattga tccggtaaaa
    18121 ctggcaagcc tggacatcct ccagttttat tccgaaatct atcagacggt tcaaaacccc
    18181 gcagaccaat gcaggcccat cagtcaaaag gaggtggtca tccaggacac gcggggtatg
    18241 gaattttatg cccagtgtgc agaacgcgag gatgtcccgg aactccggta caaaaggttg
    18301 gtcctggtcc cgcctaacct ctacgtcatt gatttctgga ctacccgtgc cgcctccgat
    18361 caagccgccg gtaggaaaaa tgcctttttt gaatccatga tcttcacacc gcaaggaggg
    18421 caggccgtga tcccatccgc cctggataaa acggaatccg gcggttccgg ctggacgcaa
    18481 tacgggtatt tcctggccgc catcgcggtg ctgctggttg cactgctgta tttgaagaaa
    18541 aagcctaaga aatagttaat aaacccagcc cgcacccatt gcctggcttt attcttattt
    18601 ttgccgcgca aaagatcatc acctctccaa tgaataaatt tttattctgg tccgtattgg
    18661 gacttgtttt tatcagctgc cagcaaagtc cgcgggaaat cgtgattgcg gaagaccgta
    18721 agtccaatta ccggatcatc tttgcggaag aagccccgga cagcctgcgt acagccattg
    18781 aaatacttca aaaccacctt cggctgatga ccggagcgga tgtgaaagcc tattcggata
    18841 tcgttgttcc gcaggccgaa gaaatactgg tcggaaaaag caagcggatg gaaaaatatc
    18901 tgaataagat ccctcttgcg gatttgggct cagggtatta tgaaggggtt cacgacaaaa
    18961 aatggatcat catggctaac agcgcctccg ccatcctgtc cgggatttac gatattgtgg
    19021 gcaggctggg aagcgtaaaa gtcagtcagg acctcaccca gtttacccag gtgcagcggt
    19081 ttacccgctc ctcggatcct tatatattca aacccacttt cagctaccgg caggcggtga
    19141 ctcccctggc caacaacgac caatatcggg cattcaacca gatcaatatc aacaacaccg
    19201 atgactgggg cacctgggct tattccatgg agcggatttt tcctgccacc tcctacttca
    19261 gttccaagcc gcagtatttt gcagaaatcc agggaaaacg tttgcctgcc caggttaatt
    19321 tcagcgaccc gcgcatgaag gaagacctca ccaaaaacct ggaggtctgg accatgagca
    19381 agggcctggc ttcctattgg tccataggac cctattccaa caggatcgtc agcgaagacg
    19441 aattaacccg caaggccatt ggagagaccg gaagccccag cggcgccctt ctgcaactcg
    19501 tcaacgaaat cgcggcggaa aataaaagca ggacctacgc catttggctg gacgggccct
    19561 accgcattgc gccggacaaa atacaacccc tcaataatgt catggtggtc ctggacacca
    19621 gggatgtgga ccatgcggtc tccctgggcg aagggcagac caatgaaacg ttccgacggg
    19681 acctggcggc ctggaaaaag cagacctcca acatcgccgt ggtctcccat ctgaccaacg
    19741 aggagaaatt catgatgcct ttccccaacc tttatgccct tcaacgctcc ttgaaatatc
    19801 tggattcgga aggggtggat aaaatcattt tcgtggggaa ttccttcatt ggttcagcta
    19861 tgcccgacct caaattctat gtagccagca atctggcctg gaatgtggac caaccggtcg
    19921 acaccctgat caaaaggtac tgcgataata aatatggagt ggctgcagct tccatgtatg
    19981 cttatgtacg cgccctggag accacgctgc aatccaccaa gtcccggctt tatgtgaatg
    20041 gcagcccttc cgacggtttt cgaagctggc tccaacccac caacatcaat caattgtact
    20101 cgtatttcag cgccaccacc agtgtgaccg gcaacaaccc ggatttaagg gatctcgtcc
    20161 tcctcgagcg cctttccctg atttacaccc agctggaagt ggccaaatca atgggcacca
    20221 atacctatgg ttattttatg aatatcggcg ccctgagcca attgatccgg caggaaaata
    20281 ccatgcgcca ggaaaaggcc accatggata tccgtaaaaa agactggggg accatacagg
    20341 gcatgcggga cctgctcgac cagtttgtca acgactgtga ccgcatgtcg gtaagggtag
    20401 tcgatgcaaa gggaaccaca cccgcacaat accgcaccct ggtccagaaa tatatagatc
    20461 aacaaatcca gacccatttc gcctttaagc ggggcacttt tacctttaat gtttctcctg
    20521 acccttattt tgccgatggg gccgaaacca tcctttacga cggggtggcc ggcctggcgg
    20581 agaacccgca aaccaactgg atgggtgttt caggcggagc atttgaagtt acctggacca
    20641 acgacaagga taccatcctc aagtccctga ccacccgttt tcttcagaat acggaagtaa
    20701 gggcctggtt gcccactgag gtaagctgcc tggtttctgc cgatgggacc tccttcaccg
    20761 aagtaaatac ggtccggttt ggtcccgaaa ccctggcaag ggccatccgg ccggtcacct
    20821 ttaatctggg caaccgcaaa atcaaagcta taaagctcaa agcttccggg ctcaaaacct
    20881 gtcctccgga caatatccag tccggcctcc ctgcagccct ggtggtggat gaactggaaa
    20941 tccgatgaat ccatgattgg aacctggtgc aggcgccctt tagggagcag gcttgcaccc
    21001 gtttcagggt ctccgattaa aggggaactt tagtttgtaa cttcgcggcc atgatcgatc
    21061 ccaagcgccc cttccgcctg accaaaaaaa cccgttttta cctgcattcc ctggtccggc
    21121 acgacctcaa agccagcatc accgtatttt ttgtcgccct tcccctctgc ctcggcattt
    21181 cccttgcctc caatgcgcct gtttattccg gcctcatcgc aggggtgatc gggggcatgg
    21241 tggtcaccct gatcagtaaa tccgaattga gtgtcagcgg ccccgccgcc ggactggcag
    21301 ccctttgcgc ggtggccatc accgatatgg gcaacatcca atattttttt atttcggtcc
    21361 tcctggccgg ttttttccaa tttgccctgg gattgctgaa attggggggc ttcacccatt
    21421 ttattccttc cgcggtgatc aaaggcatgt tggtcgccat agggatcctg ctgatttcaa
    21481 aacaggtccc cctgatcatc gggtacgaca aaccggattt ctggcgtgga gaattgttca
    21541 acatcatcac cttcaatcat ggattcagcc atattgaaaa cctgtacaag acctcatccg
    21601 ccggtgtggt attgctggcc ctccttgccc tggtctgcct gatcctttgg aaaaaatacg
    21661 cttcaaaaaa atttgaagta ttgcccacct cctttgtgac cgtactgttt ggggtgatca
    21721 tttccctcct cttcgcttac tggttcccgg cgctcgcgct ggaacccagt cattttgtgc
    21781 gggtgcctca ggatattttt tctgaagtgg gatggccgga tttctccatc ttgtttaaat
    21841 ccggggagat cagttcgctg atctggaaaa atgcattgat cttctgtttt atcgcttccc
    21901 tcgaaaccct gctttccgtg gccgccattg acaaactcga tcctttcaac cggatcaccc
    21961 cgccaaaccg ggagctcatg gcccagggga ccggtaattt cctgagcggc ctgctgggag
    22021 gcttgcccat caccgcggtg atcgtacgca gcagcgccaa tgcggaagca ggcgcaaggt
    22081 ccaaagcatc ctccttcctc catggatttt ggatcctgct ggccatcctg ctggccatac
    22141 ccctcctcaa ccggatcccc tatgccgtcc tggccatcat cctgatccgt accgggtaca
    22201 atctggcaaa acccaaaatg atccgggagg tatatcacca ggggcgggaa cagttccttc
    22261 ccttcatctt caccatcgtc gccatcctct tcaccgatct gctcatcggg gtggtcatcg
    22321 gtattaccta cgccatttat ttcctgttta aacataccta tcacgccggc ttccaggtaa
    22381 aacaatccaa ggaaggacat agcccaaaat tcaccatcga actggatcag cacgtcagtt
    22441 tcctcaacaa acgccgcctt actgaaaccc tggacaaaat tcccgaatac gccatcgtgg
    22501 aaatcaacgg caccgacagc gcctatatcg accgggacat cctggaaatc gtgcaggatt
    22561 tcagatccaa agcccaccgt aagcacatca gcctgagcac cgtccaggtg ccggaagtga
    22621 ggacgattga attgcattaa agtcccggct cccatagtaa aatgtaacta ttatttcgag
    22681 acgcttcaat ccctgaaggt ttatataacc caaaccatgc aatagaattt ctattacaaa
    22741 ccggaatgcc tgcaaatact ggcacttcct cccattttta tcctcaccat aggagtgcct
    22801 atgtttgcgg taaaaatagt ccgggagcgc cagtctttat tggccttccg gttttcatta
    22861 cgaaactcta ttgcatgatt tgggtttaag tctggttcca ggcgtcgcgt ccggcaatat
    22921 acttggactg atgtaggtac ctatagataa aggaaatcac atgagtttgc ctttcaggat
    22981 aatgggctta tccttttagt aagttaaaga gagcttcatg aatgaaaagg ttttctttcc
    23041 aaacctgtct cgtttttaag atgccagcgc ctgccaattc attcagataa cgggaagcag
    23101 tcggacggga cacgtcaagc tgctccatga ccatctcaat gcggcaataa ggctggacaa
    23161 atagcaactc caaaaggtct ttgctgtata ttttcggttt ttccttccta actgtttcca
    23221 gtgtgcgata aaacaggtta ttgatttgac gaattttctc aatcgtttct cgggctgata
    23281 cttctacagc tttagtcata aaaagcaccc attctcccca attttcacgg atgcgaacct
    23341 cttgcagcag acggtagtag tctgatttgt gatcaatgat aaatttgctc aagtaaagta
    23401 ctggcttttc cagccagcct ttcataatta agaaaaggac atttaaaatg cgccctgtcc
    23461 ttccattgcc atcgtaaaat gggtgaatgc tttcaaactg ataatgctgc acagccagtt
    23521 ggatgaaagg ggaaaggttg tcgtcgctat ggaggtaatc ctccagattt ttcattaacc
    23581 ggagaatggt ttcttgatga tcaggcgggg tataaatggt tgttccggtc gccgcattct
    23641 tcaatgcagt gcctggcaaa cggcgaatgc ctgcgctgtt ttcttccaac acttgctgaa
    23701 ttttgacaat atcattcgta ctcaaaaaac ctcgtttcga aagtaagcga tatccctcca
    23761 acagggcttc ccggtaacga aggacttctt ttgtcgaaag gtcaacgttt aacctcttga
    23821 tagaaagcgc ctggtaaagt ttgtcttggg tggttataat gttttcaatt tcggaactgg
    23881 cggtggcttc tttcaaaacc actgcattca gcaaaatatc gggattgggc agggtgttcg
    23941 ctaacccttt tagttcgcca atggctgcag cagtatgaga aacttgcctt agcaccggga
    24001 ttgtttcgat ttgcgctctc ggtggcggca aaaaagggag gtcgttgaat ggtatttcag
    24061 gtttcatcat gcccgaattt aggaaactct ggttatatca tataaagaaa atcgaaatta
    24121 ctttacatga caacgttttc atactaagtc tttttacatg accccgcctt gaaagattgc
    24181 tccgagtcaa tccgggtcca catcgatatt aaaccgggtc gaagacagcc cgggttgcct
    24241 gaggacttca taaatgctgg tgcggaggga agattttatt tcctggatga cttggctgtt
    24301 tttctccatt ttgatcagga tgttccgaag gtaatagttg cggagccttt cgatggacgg
    24361 gagcaaaggg ccgatgaccc gttcgccaaa gcgggactgc aactgctttg cgagcatcag
    24421 agaggcctgg tccacctgct ggggcttttt atgttttatc gtgatttcaa taagcctcat
    24481 gaaaggagga taacccaggg cctgccggtc tttcaattct ttttcagcca gcccggtgta
    24541 atcataattc atgatgtatt ttaatacttc gtgcctcagg ttgtaagcct ggaggatcac
    24601 ccgtccctgc ctttctttcc ggccggccct ccccgccacc tgggtgagca actgataagc
    24661 ccgttcattg gaccggaaat ccgggtaata aaaaatggaa tcagcctgca aaatgccaac
    24721 caggcctacg tgatcaaaat caagcccctt gctcagcatc tgggtaccca ccaggatgtc
    24781 aatatttctt tcttcaaact gatcaagcaa ttcctggtag gaagcttttg acctcgccgt
    24841 gtccagatcg agacgccgga cccgggcatc gggatatttt tgtttaagtt catcctcgat
    24901 cttttccgta ccaaaaccac gcagggccac ggtgggtttt tcacaatccg ggcaagtgct
    24961 aaacaaagcc ctccggtaac cgcaatagtg gcagaccagc tggttgatgt acttgtgata
    25021 ggtgaggctg acatcgcaat tggggcacat cgcatgccaa tggcagttgg aacaatgctg
    25081 ctggggggca tagcctctcc ggttttgaaa caggatgacc tgctcttcct ggcgaaggga
    25141 ccggaggatg gcatcgtgaa gggtgttgct gatgatgtcc ggcggatcct tgccctggtt
    25201 ttcccggtaa agatccacca gttcaatctc cgggagttcg acttcctggt atcttttttc
    25261 caggagggca tgtccgtatt tatgctgccg ggcatggtac caggattcaa tggaaggggt
    25321 ggcgctgccg aggatcactt tggccccgca ttgcacggcc agccatatcg caaggtcccg
    25381 cgcatggtat cgcgggtccg ggtcgtattg tttataggaa ggatcgtgtt cctcgtccac
    25441 gacgatcagt tttagattgg tgaagggaag gaagatgccg gaccgggcgg ccagggtgag
    25501 cggggcaccg tgaaggaccg cctgccaggt atccaccctt tcccgctgac tgatcctcga
    25561 atggtacaga tgcagatgcg ggcccagcgc cttttttagc ctggtgatca ggtgggtggt
    25621 gagggcaatc tcaggcagca ggtaaagcac ctgcccgccc tggctgaccg ttttctcaat
    25681 aaactcactg tacacccgcg tctttccgct tccggtaacc ccctggagca gtacgacgtt
    25741 tttcatatcg ccgaaagcgg cgctgatctc aaaggacgcc ttgctctggt cggctgtcat
    25801 atccggtaat tcaatcttca attcagcggg gaggcctctg gccgccggcc ttttctggag
    25861 gataaaaatg cctttcttct ccatggcttt gagcaccgga gtggtcaggt tgacatgcct
    25921 ggccaggtct ttcctcagga tctcctgttt tggacgggca tactggatga aggcaagcat
    25981 cacctgggtc tgcagggccg atttagccac caggtcgagg gcaccgacca tccggtccgg
    26041 gtccccctgg aatttcgggt ccaggacaag gtattcttct tttttatcct gcaggccgct
    26101 cagcaattct tcttcgatca ggatgatccc tttggtgacc agttggtcga tgattttatt
    26161 gatggacttt ttctgaagga ttttgcgcat gtcctcaatg gaaatggcct tgtgcaggga
    26221 aagggctttg gcaatgaggt attcctgatc atcgagttcg gaaaggtcca ggtcaaaatc
    26281 gggatgcagg acgatcttgg attcactgtc cagcttgtaa gccgccggca gagccgcggc
    26341 catcacttcc ccgatgccgc aacaataata gttggccacc cattcccagg ttttgagttg
    26401 aaggtccgtc accaccgggg cctcgtccag gatagaaagg atggatttgt accggggggg
    26461 aatttcggct tcgtgataga gggcggaaac caccccggca taaagcttgc ttctgccaaa
    26521 ctgtacctcc acccggatcc ccgggcgaat ggtttccata aattcacggg gaacatggta
    26581 ggaataaggc ttgtccaggt tcaaggggag gatgaccctt gcaaataaat tgtgtccggg
    26641 ttggtcctgt tccagatcgt cgggaaagaa aggcatgcca tctgcaaaat aaagactttg
    26701 atttactttt accattgatg atcttccaat tgttcctgct cgggtggtta ctcaaactcg
    26761 atactccagt ggatggtggg tggtgggtag cagatggtcc tcacccgcca ggcgtgatcc
    26821 ggcacggaat tgataaaaat caaagtcttg aaatggctca acagccgcaa tatgcctgtg
    26881 tgggccggat gagccaggga tacgtcacca agggaagttg tgtcctgatc cggcccgggg
    26941 tagcgctcac agcggcacat accctgacca aattgcaccc cacactgccg ctcatggccg
    27001 tttttgacag catggaagtg aaggtggatt ccttcaggat ccatccctgg tattatgaat
    27061 tcccggaggc ggatctggcc atcctctatt tgtcagtgcc tttaaaccat attcccttac
    27121 ccggcctcaa ccggaagaaa aaagagacgg ggcaaacagg cacgacggtt ggattcggca
    27181 atttcagcgt ggccaacgac cccagtcata ttatagacgc gggcaagctt aaatccgccg
    27241 gacaaaacat gctggactcc gtagccggcg ctcccctgcc tgataaaaac cggccccagt
    27301 tgtttgccga tttcgaccat ccggaagacc cccggttcaa ccggagcggc actgcggaat
    27361 gcctccccct ggagtatggc ctggatggcg gcgacagcgg cggcggactg ttcatcgtcc
    27421 acaggggcag gaccctgctg gccggcatca acgccatcca gaataagaac atagccgaca
    27481 tcatccgcac ccgctctttt tatggaagtt cgagccaatg ggtcaggatc tcggtattca
    27541 ggaaatggat caggagaaat agttagctag ttagtaagtt ggctagttgg ctagttggct
    27601 agttgcctag ttgcctagtt ggctagttgg ctagttggta agttaagcca tactggaagg
    27661 agaaaggcga actgcgaact tgctaacttc ctaacttact aacttactaa cttgccaact
    27721 aaatgttgtt cgcctttctg aaaaaggtta tgaccgggac accgatcagg aaagattccc
    27781 atttcataca ccggctgagg ttgtatttaa aatcggactg gcgcaaggga ttccgggaat
    27841 ataaaatatt cactcttcgg ggccttaagg actttatcct gatcagcatg ggtatttttt
    27901 ccgcggcctt tggccttaaa ggatttttgc tgaccaatca attcatcgat ggcggggcca
    27961 cgggcatatc cctgctcatt tccaacctca ccgacatacc cctccccttg ctgatcctta
    28021 ccatcaacat tcctttcgtt ctcctcggtt tccgggtgat cggacacact ttcgccgtca
    28081 agaccgcgct ggctatcctg ggcctggccc tggtgctcgg caccgtccct taccccgacg
    28141 tgacccagga ccatttgctg gtggccatat tcggaggttt tttcctgggt gcaggcattg
    28201 gcttgtctgt aagaggcggg gctgtgatcg acggcaccga agtcctggcc atttacctga
    28261 gccggaaact cagcacgacc attggagaca tcatcatcct catcaacgtg ctgatctttt
    28321 ccacggcggc ttacctgctt tccgtggaaa tcgcccttta ttccctcatc acttacctgg
    28381 ccgcatccaa aacccttgat ttttttgtgg aaggcctgga ggaatacatc ggggtgacga
    28441 ttatttccac ccgctccgaa acgatccgca acatgatcgt caacgaactg ggccgcggat
    28501 tcaccatcta tcagggcagg agaggctttg gtaaaaaagg ggagatcaag gacattgaaa
    28561 tcgtctatac cgtggtcacc cgcctggagc tcaacaaact gaattccgag atcctgaaaa
    28621 tcgacgatga cgctttcatt gtgatgagca atgtcaaaga caccaaaggc ggaatgatta
    28681 aaaaaagacg attaaaaaat taattcgcct gcctgcttaa tccctttctt atgctacgct
    28741 tatactttcc gaaaagatcc acggcggccc tgctcctggc ttgtaatttg ttttattcat
    28801 gcaaaacatc cggaccggac gctgaccaga tcgtggtgca tgccaggata tggtccgacg
    28861 ggggaatccg tgaatacgat gccctcgcat taaagggaga ccggatcctg gccacgggcc
    28921 ctacggaaga aatgatgaaa tacaaatccg ccgccacgga agtccttgac gcaggcggac
    28981 gcctgttgtg cccgggattt atcgatgccc atgtgcatct cctgcaggcc ggattcagcc
    29041 tgagttcggt gcagttgcgc gatgccggca ctccccggga atttattgca cgaatggctg
    29101 cttttgcaaa aacggtgcct gcaggccaat ggatcaccgg cggcgactgg aaccatgagc
    29161 tttggggcgg ccagcttcct tcgcgccact ggatcgattc ggtcaccccg gatcatctgg
    29221 tggcggtcaa ccggctggat ggacatatga tcctctgtaa ctcgctggct ttggaacgcg
    29281 ccgggatcac cggggatgtg aaagaagtcg cgggcgggga aattccccgg gatgaaaggg
    29341 gcgaaatcct tggcattttt aaggacaatg cggccgacct gatctatgct aaaataccgg
    29401 cccccgagcc tgccctggag gaccaggccc tccgcaaagc gatggaatat gtagcctcct
    29461 atggggtgac cggggtccat cacatgggca gcttcgccga cctcgaagtt tttcgccgga
    29521 ataaggataa actgatcacc cgtttctacg cagccacacc cctggcccag tgggcggacc
    29581 tcaaaaccta tatcgatcag tatgggaggg gtgattccat cctgcactgg ggcatgctca
    29641 agggattcat ggacggttcc ctgggttcgc atacggcggc catgtcggaa gacttcagcg
    29701 acaaggcggg agacaaaggg tttttcgtaa atcctcccga ttcgatggaa gcctggatcc
    29761 tggcggcgga cggggaaggc ctgcacatcg gcgtgcacgc gatcggggac cgggccatct
    29821 cggaccttat cgacatttat gaacgcgtcc gggtaaccca tgggcccaag gaccggcggt
    29881 ggcgcatcga acatttccag catcccgcgg ccaaggatct gacccgcatg cgggaactgg
    29941 atgtcatcgc aagcatgcaa ccctaccatg ccatcgacga cggctgctgg gcggaaaaag
    30001 tcatcggcaa ggaacgcatc cagctcactt atgcattccg atctttgctg gacgtgaagg
    30061 tgagcctggc cttcgggagc gattggtttg tggctcccgc cattccggct atgggcatct
    30121 atgccgccgt cacccgccaa accctcgatg gaaaaaaccc cggaggctgg gtccccctgc
    30181 agaagatatc ggtcgacgaa gccctttccg cctacacttc gggttccgcc catgcgggat
    30241 tcgaggacca gtacaccggc agtatccggc aaggccatct cgccgacctc gtcctgtttg
    30301 aagacgatat caccaccctg ccccaggact cgatcaggac ggcaagggta gcctggacca
    30361 tgatgggcgg cagaatggtt tatcagcgag atgcgagaaa cgagtagcga gatgcgagta
    30421 gcgagatgcg agaaacgaga tgcgagaaac gagatgcgag tagcgagatg cgagtaacga
    30481 gcagcgagat gaactt
//
LOCUS       DAOQXT010000053        29631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1841_length_29631_cov_5.602685, whole genome shotgun sequence.
ACCESSION   DAOQXT010000053 DAOQXT010000000
VERSION     DAOQXT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 29631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29631
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1841_length_29631_cov_5.602685"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..349)
                     /locus_tag="PKM27_10590"
     tRNA            complement(276..349)
                     /locus_tag="PKM27_10590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(313..315),aa:Pro,seq:tgg)
     gene            681..2612
                     /locus_tag="PKM27_10595"
     CDS             681..2612
                     /locus_tag="PKM27_10595"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR07751.1"
                     /translation="MKFSTLILLLSFSISTVAQNSNIEQLLVEINEAQGSDLVDKTLQ
                     LANAYYRLGEYEQAKAAGHKVMELAPGLGMDRQMQEAQDLVVRCEQITALKNSQAKSD
                     PMGSMVIALQLSDLFYKNGEFDKARQMAAESYKYAYQFDNKSFMATALNREARATLKK
                     DKPSKEEEEEARRKLRTSLKIINDHKVINPLLKNDNLVNLEELGKNFVDAEEFAEANE
                     SFKTVMDSVNTHFRIWSYEGKHRKNGKRDSGWQGAVFLAPRDSIKKGLPEDLYEKIVQ
                     LKQIRLAKELIAPLPPPSSAGKDVNLMVEQVTNELKDLWPKELQEIQSDFEKNESRIG
                     QLAPGDVKKELLQATYKNKYDSLMHLHILDSINLEKQELSIRQHEAEMERQKARRSFM
                     MTGSGSTLLLSLFLFLGFTRQKKHNRLLTLKNTEIKLAQERSEELLLNILPAQVADEL
                     KQYGESKAHRFDNVSVLFSDFIDFTRIAETLPPETLVKELDYCFKAFDRIISKHSLEK
                     IKTIGDAYMCAGGLNPSLGNQTSGIVQAAREMQQFLHQWKEEKKARGEPFFEARIGIH
                     TGPVVAGVVGMKKYAYDIWGDTVNIASRMELSSEPGKINISGETFKQLNGEFSCSYRG
                     KIKPKNKAEIDMYFVEVSG"
     gene            complement(3023..6415)
                     /locus_tag="PKM27_10600"
     CDS             complement(3023..6415)
                     /locus_tag="PKM27_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HNR07752.1"
                     /translation="MKYDSDRFTLAATDLTNHLSCHHLTQLNRKVAMGQMAKPRWHDP
                     MMDVLRERGMEHEKAYVEHLRAQGKQVVDLRGKDSEAVVEAMRSGAEVLVQAGLGEGP
                     WRGIADILLKVDGQSRFGPYCYEVQDTKLTQNTKAETILQLCLYTDLLSGIQGGEAES
                     MSVIKRGNPFEREAFRYLDYRAYYRLVKKRFEETMAGPDLPTYPEPVEHCQICSWWQE
                     CDKKRHMDDYLSLVAGMQSIQIEELKSQGISRLEQFAETDLILKPGRGNLEALLKRQV
                     QARIQLEGRRRQKLLSKTLESEEGRGFHRLPEPNEGDIYLDFEGDAFFEGGGLEYLIG
                     YAVKKEGGLDYRRIWSSNKHEEKQAFSRFMDFVKAQWEEFPGMFIYHFAPYEPSTVKR
                     LAMIHSVYEAEVDQLARGLRFIDLHQVFKESLLASVETYSLKELEKFAAYERKLELHK
                     ASSARKRVEVALELNDFLSLDEQVIAELEAYNEDDCRSTAALHQWLEEKRKTEINLGK
                     PIERPLLKEVEARDETKDLDERTRKLFLALTENLNSESKDTEQLARRLLANLLDYFRR
                     EDKSAWWEYFRVHELEYDDLLEERKAIAGLEFVEELPKGPREKNPTHRYRFPPQEIRV
                     EVEDALDEVKGKSIGQVKAISLENNTIDIKKSGLAKERHPRAVHVPERVDPRLLTDSL
                     AAVARNLLDHGFNGQGSCRAAFHLLTKMRPDLLYNYDGPLLLQKEDLESGARRLATSL
                     NSSVLAIQGPPGTGKTFTGAQMIVDLYKQGKKIGITAISHKVIRNLAKSVIKHKPVDL
                     EIFFVHKGSVGDPEEIPFIREVGESDKVRAALEEGKIGCGTAWLWSEDKSEGVLDYLF
                     IDEAGQMSLAHALAASRASKNLVLLGDPQQLEQPQKGAHPEGSDIAALSHLLDGHLTI
                     PGTKGLFLGTSWRLHPEICKFTSELFYESRLNSKPGLERQRISGTRGFDEAGLFYVPV
                     PHKGNQSRSDEEVAAISAIVGTLLAGGFWTDEEGKTNNLMNKDILIVAPFNAQVGALR
                     QKLPGIEIGTVDKFQGQEAPVVIYSMTSSSAEDAPRGMNFLYNLNRLNVATSRAKCIC
                     ILVASPALFEPECHSIEQMKLANALCRYKELAKEVVLDALDG"
     gene            6551..7342
                     /locus_tag="PKM27_10605"
     CDS             6551..7342
                     /locus_tag="PKM27_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR07753.1"
                     /translation="MKYKWLGLGLLTIGIHASLPSFSQGYVNTPPAVSPMPMKPEMTE
                     IWNPEVPVVKPGKNLGEAPSDAIILFDGKNLDQWVSQRDPSKPAPWKIVNNDHMEVVP
                     GSGGIQTKMQFGDCQLHIEWSAPDMVESGGQGRGNSGVFFQNRYELQVLDSYNNRTYR
                     NGQAGSIYKDHAPLVNAMKGPLEWNVYDVVYTAVRFKPDGSVFSPARITVFHNGVLIQ
                     NNVTINGITEYIGLHMYKSAHGDDVLSLQDHNNKTQFRNIWVRPL"
     gene            7786..8910
                     /locus_tag="PKM27_10610"
     CDS             7786..8910
                     /locus_tag="PKM27_10610"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNR07754.1"
                     /translation="MKKIYLISCLSLILISVSGPLQGQLLRLPDANVTNLKASVGRTV
                     GVTDIMIRYNSPAVRGREGGIWGTAIVPYGYNVLGFGSNMESPWRAGANESTTISFST
                     DVTIQGKPLKAGQYGFFIAVYPDSCILIFNQNTKGWGSYFYDKGLDVLKVATRQQKNM
                     PASKERLEYAFSNQTDRSVEIALEWERWRIPFKVEVDIDGTTLASIQSQMSGAMGFDP
                     PSLQAAAAWCLSHNTNLDQAYTWITSATDPNLGGLKTFAALSTKAGILGKMNKKEEAD
                     QIMAQAVEQANAFELHAYGRQLLAQKKMKEAMDIFETNYKKHEGKWPTAVGMMRGYSA
                     MGNYPEALKYAKMGLTQAPDDLNKNSLQDAVKKLEAGKPL"
     gene            9041..10558
                     /locus_tag="PKM27_10615"
     CDS             9041..10558
                     /locus_tag="PKM27_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07755.1"
                     /translation="MKQWIMPLAFIFILPWSTFSVTGQEWMNLSLNYGPYKTGFRYIE
                     TSDPGRPCLTCAKSVTGYAAREMEIAVWYPSEDRETATMPYIQYLRLGPAGTITDLNI
                     LKQAFFKSISGLGGDTSLFETAFAPLFHEATRAQKDPTLAPGRWPLILYPDQPHFQNT
                     LCEFLASHGYIVASPRIKGTFRAEPEYNPAGIETGTDDLAFTLAYMRSHFQVNREFAF
                     IGTGFNATLGLALSFKNKDLAALVSLEGGITTPFEAGLILKSPYFDLRQCSVPMLVIH
                     APHPDVKPELTHRYKYSERVYQSYPQSSEFYFLNFGLWEKKVRNIFPKANRANTWESF
                     EISAQSVQLFLDWKLKKDEKSKSLLLENDWPPELMQTSVRPAVPIPPDHEQLSEVWEK
                     KGIDSLNMIYRNLRAYDNQPFDFSSFYQLSQKMIEQSRFRDLLSWALLFADAHPGSAI
                     PYSLQGRAHLELSNKAEAKKNYEKALSLLGQDPELNAEEKNYFGQAIETRIRSLN"
     gene            10571..11002
                     /locus_tag="PKM27_10620"
     CDS             10571..11002
                     /locus_tag="PKM27_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR07756.1"
                     /translation="MKPLFVIAALALSFPIQAQSPEDSVAIKKAALDYVEGFFTADSS
                     RLTQAMSPELTKRIIDHRSGKSKLLTIGLKELIGYTRMGKIPDKNPDEPFKAKVEIYD
                     IAGGIALAKVSTNKMPEFFDYIQAGKMNGEWKIINVLWAFN"
     gene            11165..12163
                     /locus_tag="PKM27_10625"
     CDS             11165..12163
                     /locus_tag="PKM27_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNR07757.1"
                     /translation="MKIHTCTFLLLLLSPFSGRKELEFAPTARITSSPQKLNNEKPGL
                     KNIIFKSTDGGQTWQDISEGLPENFKENGFYANDQGIYLLYGNGVYHRNINSQSPFWE
                     KEILINKQPNIAPGDSSLFAYGNDGPFLQKINGTRVWSPLYSNFQGGAIHALLETKGG
                     SVFICSDRGLYKSDKSGKSWKQVHTGGWVMKLAESNGVLLATSQGGILRSTDEGEHWD
                     LVISEGGVGIAVECINGGFAAITYNTESKTRRVRISYDGGKTWSPIDAGLPPSLSIAS
                     IIQAGENFYCGHPDGIFRSSDQGKSWSLMLPSVENKVFNLTVSGKVIYAIPRDGGC"
     gene            complement(12199..13683)
                     /locus_tag="PKM27_10630"
     CDS             complement(12199..13683)
                     /locus_tag="PKM27_10630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR07758.1"
                     /translation="MLDIKKNITLFIILLIGFVLRLTAIFTDPFLHPWDERFHALVAR
                     NLMDHPLIPVLRANPVTDNYDKFIWCCNHVWLHKQPLFMWQMALSMKVFGVSEWSMRL
                     PSAVMGTILIFLTYRIAILLTKNRTVALFSALMLAVSGFHLQLIAGMHGMDHNDVAHG
                     YYVLASIWTYCEYHRNQKWYWVVLTGVFSGAAILNKWLTGLLVYLIWGVVILITGLQK
                     SSVGKQVIHTFLSLFFCFPVFLPWQIYILDRFPDLANFVYDFNKRHITEALEGHTGDN
                     SFYLDHLVDLIGKYLYYFIPVGLLLSFIKKEISGRLNAGIIAGMLFVFWFFSVFVKTK
                     VDTYVFFIVPLGLIYISLGLNYIFEKLGKSRLVLAALVLATVYLSLNPSWFINYLSPQ
                     NNERNIRIYNTEIYKALNRLLPPNVKTVVNMNEFEDIDVMFYNEGITAYHFTMTEQDF
                     EELKVKQIPVGIFIPHGKYIWPKYVEDYPYLYPIDQELKNLDNK"
     gene            complement(13834..15180)
                     /locus_tag="PKM27_10635"
     CDS             complement(13834..15180)
                     /locus_tag="PKM27_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR07759.1"
                     /translation="MKKNHAWILLLFLSSLAQSQTWTSLFDGKSLKGWKQLNGQARYR
                     VENNQMVGTTVANTPNSFLATEIEYGDFILELELKVDNSMNSGIQIRSLSLPSYKEGR
                     VHGYQVEIDPSDRAWSGGIYDEARRDWLYIPDINPEGKKAFKKDQWNKYRIEAIGPVI
                     RTWINDIPVACLVDDLTLKGFIALQVHSIGKDQAPGTEIRWRNIRIQTSGLKPRPYDQ
                     TPVVNLMNNHLSEMEKNQGFKLLFNGNDFNGWRKVHQTGPPEKRWSVKEGMIGVMKSD
                     GSETGNDIVTTDQYGAFELTFDFKLTEGANSGLKYFVDEQYDSGGRSGIGLEYQVLDD
                     ARHPDAKQGVVGNRTLSSLYDLIPATPLNSRFQRKIGDWNQGRIIVYPNNMVQHWLNG
                     FKVVEYERNSNIFKALVARSKYAVYPGFASAAKGHILLQDHGDEVWFRNIKLRTLE"
     gene            15318..16973
                     /locus_tag="PKM27_10640"
     CDS             15318..16973
                     /locus_tag="PKM27_10640"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNR07760.1"
                     /translation="MNVLSPSPLPTTDPEPTDPRRLAGMLRQNLDPYPGPWDEKGLKH
                     LLRRSLFGTSAAEVEQFLLLGNLENTLSALCEIKPPASLPLVNYTDGDGVREPNAKDG
                     ETWIHEEYNNELEYYRLLSLKTWWLDLMLKDQTLMEKMVIFWHNHIPVEMVGVFHARQ
                     AYDYLATIRQYALGNFRQLIRALTLDPMMLFYLNGNSNNKSAPDENYARELQELFCIG
                     KGPEAKFTEDDVKAAARVLTGWKSDYLKPKTFFAEWAHDTGDKTFSDFYGGRVIRGQK
                     GSEGQKELDELIDMLISHPECARFICRKLYRFFVYPEISDWSEENIIRPLADLFIKTD
                     FEIAPVVKALLSSNHFFDPLLRLAMIKSPLDYTIGLNRHFGMRFPSSPGEIKSKFQAQ
                     TVMYYFLTSFQQDIGDPPNVSGWPAYYQFPQYDRSWISTHTVHQRGIHSDMLVWYGYQ
                     MDQGVATIDWPAYTDTLIHPENPNLLIEEVLGRLYDVPVDAEVKKYLKSILLSGQVSD
                     HYWTNAWQEWKSKPNDPMARNTVDIRLKIFYQYILQMDEYQIM"
     gene            16979..18550
                     /locus_tag="PKM27_10645"
     CDS             16979..18550
                     /locus_tag="PKM27_10645"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNR07761.1"
                     /translation="MKRRDFFRLTGSGLAANSFLPGFRPDKIPQWFDWFKLDTDRVLV
                     LIQLDGGNDGLNTVIPLDQMDLYNKARQSVALPANKVLSLPTTIKSGLHPALDEIRGL
                     YTEGRIKIIQSVGYPSPNYSHFRSTDIWMSASDAEEVLSSGWAGRYLNHEYPNYPNGF
                     PTEDMPDPLSVEIGYGQSLAFQGPATSMSVTIANPEDFNKLIQGIETPLPQTPFGDKL
                     GYVRLIKKQSNTYGERMKDAFGKTKNKASYPEDNDLADQLKIVARLIAGGLKTRIYKV
                     SISGFDTHDNQVQNGDHATGEHANLLRRISGAVKAFLDDLTALGLQDRVVGMTVSEFG
                     RRIISNASRGTDHGAAAPMFLFGNAIQGGLLGENPTLPAEAKWDDNIPMQYDFRSVYG
                     SLLENWFCINKTEVQQVLLKNYQTLPVIKPAYSCLSTPTREENQLKGISIIHAFPNPF
                     TSIINFKVQSNGEHSSISIFNPAGQVIHNIHHGLLSKGEHQYYWNSEDLPVGNYYVRF
                     QSGPEQQVKLIVKVR"
     gene            complement(18920..19918)
                     /gene="metX"
                     /locus_tag="PKM27_10650"
     CDS             complement(18920..19918)
                     /gene="metX"
                     /locus_tag="PKM27_10650"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNR07762.1"
                     /translation="MAENKVYPTGLPFYMEGGATLAGLDITYTDTGGQNKPVVWICHA
                     LTANADPSEWWPGLVGTGRLFDPEKYRIVCANILGSCYGTTGPASIDPLTGKAYGTSF
                     PLVTIRDMVHAHRLLQKHLGIDHIFLSIGGSMGGQQVLEWAITDPYLFERICLLACNA
                     RHSPWGIAFNEAQRMAIEAGIDAEGNVNPLGLEAARSLAILSYRHYITFDLKQYETED
                     KIDDFRASSYQRYQGLKLSRRFNAYSYITLSKAMDSHHVGRKRGGIPHALSLVKAKTA
                     VIGINTDVLFPLAEQKLLADQIPGATLDKIHSDYGHDGFLLEYEDIQKVLEKRFQL"
     gene            19974..20393
                     /locus_tag="PKM27_10655"
     CDS             19974..20393
                     /locus_tag="PKM27_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNR07763.1"
                     /translation="MKITLRRIGDPLHFEAANAQGNTIEMDAKAASGGTGKGMGPMET
                     LISALGGCSCIDIVLFLKKFRQELADIRVEIEADRDPDHIPSLFVKIHAHYHLYGKLD
                     ARQVEKAISLSIDKYCSVSQILKKSVPVTYAFTIHSL"
     gene            20390..21568
                     /locus_tag="PKM27_10660"
     CDS             20390..21568
                     /locus_tag="PKM27_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNR07764.1"
                     /translation="MSSRKFKDETIAIRTQTERTRHGEHSTPLFLTSSYAFDSGDEMA
                     DAFAGITEANVYSRYTNPNVEELVKKFCLLEEASAGLATASGMAAVFSSIAGLLNQGD
                     HLIASRALFGSSIQVLSHILPRWGITCTYVDPVDLSGWEQALTPNTRMVFLETPSNPS
                     LWITDLAGAGAFAHDHNLILAVDNCFASPVLQKPMKFGASLVIHSATKYADGHGRVLG
                     GIVAGKEDLMEKIAFFNRHSGPSISPFNAWVISKSMETLALRVRAHCKQAEELASRLQ
                     DHPGLEAVIYPFSENHPQVDLARRQMEAGGGIVCIKVKGDYAKTLQFINHLEMISISP
                     NLGDTRSIATHPTSSTHSRMTEEDRLTVGITPNLVRFSVGLEHIDDIEEDIRIALSKI
                     "
     gene            21580..22353
                     /locus_tag="PKM27_10665"
     CDS             21580..22353
                     /locus_tag="PKM27_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07765.1"
                     /translation="MKKGRNLLWLAFWSCLLFYQCGPAGDEKDPQYLADLAIKAHGSK
                     VFDQSIIEFKFRDRYYKSTRKDGKFIYERFWDSVGTVIHDVLDNRGFSRIVDQTEVIL
                     DAKKKEAFSNSLRGVFYFFGLPFQLNDPPVIKEYLGEVMLMEQPYHKLKIRFKPDPAD
                     QLIHDDVYVFWIHKKNHTVDYLAYSYTEPNERGLRFRQAINPRKIEGLRVQDYINYKP
                     IRDSSSLKVEDMDMAWAGNQLTEMSRIINENVKIRLNKN"
     gene            22450..24612
                     /locus_tag="PKM27_10670"
     CDS             22450..24612
                     /locus_tag="PKM27_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR07766.1"
                     /translation="MREKLLFTTVFVWLSCQFVFAQKISIGLASGKDAINEKCGLLNN
                     DQLVFTLDSAAKQNTRFVTNLGGTAIRGTDYATNLTDTVLFTTGQQSRSFDLTVFGDA
                     FAESEETVVVIATGQDGFADTFLITIQDRIARILTSQDTFRQCSNEPFKLNLYRAAGS
                     VINWFPADMAAPTDDPNVFTILPVRTSPVILTATIDQCVERDTIQVISQPIGVTLNTK
                     DTLFLCFPDSFRLVATLTPPNAQISWSPLDSTTRVINASSIQVKPPKSARYFVNAVSG
                     VCKAADTVYVSMDSLRDTKIIHFPVKDKYCPGDSIFFFSQRHPKDLFPALKPMWDPSN
                     GFQTPSDTFNAVVSADKTTTYLRTTRNNACVHRDSVYIKVVEPTIPVGPVDTTVCPGE
                     TVQINFNKDSSVYEDFKWSPQDGLISCEKCDNPKILVLGQQQYMVEAKKDGCDATAMI
                     STSVFQKPQVGVFTNIPQPIPAGSNVQFLLQLPTGTKAFKWSVDRNAVPSGNLTTTVN
                     NIGPGVHEVDVEITDANGCTWSYQFLVEVLCPPNALALQRSPQGNIYERSTLQISATG
                     INSNVTNVRWSVNGSNLNENGLTLTHTPSTAGNYIYRFEAIDLNGCPLAASITVQVIP
                     CISPEELKKKIPNAFSPNNDEKNDYFNYSDGTLTITKLLVFSRWGQLVYNNTDPENGW
                     DGRVNGNEAASDVYIYRLTYICGDAAPQEVSGTVTLLR"
     gene            24641..25924
                     /locus_tag="PKM27_10675"
     CDS             24641..25924
                     /locus_tag="PKM27_10675"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNR07767.1"
                     /translation="MKSFLPILPLLLFFGCGQGRQSQTNLPGKAETAPMREVVLMPKT
                     EAGLRLMADELAHKYLIFDGHVDLPYRLNIKNFRPTKEFIGIPVESKEGDFDYVRAKK
                     GGLSAPIMSIYIPASYQKGGAKSFAEELIDMVNSIIEGHPDKFAPGLTPDQVEANFKK
                     GLISLPMGMENGAPIENDLRNVAHFYDRGIRYITLTHSEDNQICDAAYASTRTWKGLS
                     PFGVEVVKEMNRVGMLVDVSHITDSSFWQVLRVSRAPVIASHSSCRHFTPGFERNMSD
                     DMIAALKGNGGMIMINFGSDFLDNEIRLKNNRDRNELEALLAAQNLSHRDSAAKPIIK
                     QFEASHPSLYSDVKRVADHIDHAVKLAGIDHVGFGSDYDGVGDSLPVGLKDVSAYPNI
                     IYELLSRGYSEEDIAKICSANFLRVWRKAIEVAGN"
     assembly_gap    26084..26183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26239..27159
                     /locus_tag="PKM27_10680"
     CDS             26239..27159
                     /locus_tag="PKM27_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07768.1"
                     /translation="MKLRLLFMVLPALTWFACQPAQPTGPIVEKGKDFDSYWHQGKAE
                     ITSYDLFQARYGEMREGEAVIVFVTEEHDREKQVKLDQPGIDPALKVGVLKMNLTKRF
                     YTGLYPYHMMLSSFVEAGGIAPWQGLKVAASCMEWCGQTYAQLNRRNDHYQYQLHSYF
                     EKENQIDKHLNRHMTEDAVWNLIRINPNALIQTDSILIIPSLLFARLSHIELKPYRAR
                     TSHLSLDLGNKVYHIEYPSLGRSLYIEYEPEFPHQIVRWSEEYKDGFGENAKVLTTTA
                     RRKKQIMSDYWNRHGIVDDSLRMELDLMKL"
     gene            27214..28032
                     /locus_tag="PKM27_10685"
     CDS             27214..28032
                     /locus_tag="PKM27_10685"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNR07769.1"
                     /translation="MNIFQAILLAIVEGITEFLPISSTGHMIIASSAMGIHTGAFTKL
                     FEVAIQLGAILAVLVLYRKRFLQWTNYQFYLKLAVGVLPALVLGFLFSDQIDAMLESP
                     FVVALSLLLGGIFLLFIDSVFSHHTIHQEGEIGYKNALIIGFWQTLAMIPGVSRSAAS
                     IIGGMQQKLDRDLAAEFSFFLAVPTMAAATLYSLFLKSWMVDGVEQKGFEIITGTTNN
                     TLVFLTGNVVAFIVAMLAIRFFIGYLQKHGFKLFGYYRILVGVILLILIKANKL"
     assembly_gap    28295..28394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            28541..28990
                     /locus_tag="PKM27_10690"
     CDS             28541..28990
                     /locus_tag="PKM27_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07770.1"
                     /translation="MKSPKTLVFLSLLSCSQPSKTDPRSLAKEIESADLAMSKLAGDI
                     GFNNAVLQYADSSIVKLSEGKPPIEGITAFSKSYDHEKDIKTISWTPSHAEVAQSGEL
                     GYTWGTWTLNLPDTVFLGKYFTVWKKNARGEWKVVLDGGNGTNPPPN"
BASE COUNT         7629 a         7807 c         6852 g         7143 t
ORIGIN      
        1 agcagaacaa tattacaaat ttgattttta tcaatatgtg cccgcatgac cacaatagga
       61 gggaaaactg atattggtca ggttggaggt cagaggtagg aggttagagg ttaaaggtca
      121 gaggttggag cttagaggcc ttgggagcag tgaccggctt cctgatcggt gtataccccc
      181 taaattttgg agcaaagaat ttcatttaaa cggaccattc cggtttaaaa tgacaaagag
      241 ccgcaataaa cgtattgcag ctctttgatg atccgtcggg aagactggat tcgaaccagc
      301 ggcctcctgc tcccaaagca ggcattctac cgggctgaac tacttcccga gctaagtata
      361 aatccatgac tttcagccag tcatgaaaaa agcgtcgcaa agataacatt ttcctgatag
      421 gaatggactg aaagggaatt agatatctta agttccagac ccgcttcttc aatttcaaca
      481 gacgaccggc cttccaggtc aacccttgac cctatcattt ggaactgacc tcaatggctg
      541 gtcaaacggg ctgaccgaaa acgtccgcac aatggcctcg tctctcgcgt tccggttcct
      601 ttccattttc ccctttatgc atttggcact taactttaca cctgtgtttt tctgatcgaa
      661 cagagccaaa aattttgtat atgaaattca gtaccctaat ccttttactc tccttttcta
      721 ttagtacggt tgcccagaac tcaaacatcg agcagctcct ggtggaaatc aatgaagcgc
      781 agggctcaga cctggttgac aaaacgctcc agcttgccaa tgcttattac cggctgggtg
      841 aatatgaaca agccaaggcc gccggtcata aggtcatgga gctggctccc ggcctgggga
      901 tggaccggca aatgcaggaa gctcaggacc tggtcgtccg ttgtgaacaa atcaccgccc
      961 tgaaaaacag ccaggctaag agcgacccca tggggtccat ggtcattgcc ctgcagcttt
     1021 cggatctttt ttataaaaac ggggaattcg ataaagcccg tcaaatggct gcggaatcgt
     1081 acaaatatgc ctatcagttt gacaataaat catttatggc cacggccctg aaccgtgagg
     1141 cccgggccac cctcaaaaaa gacaaaccca gcaaggaaga agaagaggag gcccgaagga
     1201 aactccgaac aagcctgaaa atcatcaatg accataaagt aattaatcct ctcctgaaaa
     1261 acgacaacct ggtcaacctt gaagaactgg ggaaaaattt tgtggacgcg gaagaatttg
     1321 ccgaagccaa cgaatcgttt aaaaccgtga tggactccgt caacacccat ttccggatct
     1381 ggtcttacga aggcaaacac agaaagaatg gaaaaaggga ttccggatgg caaggcgccg
     1441 tttttttagc accccgggat tccataaaaa aaggactgcc cgaagacctt tatgaaaaaa
     1501 tcgttcagtt gaaacaaatc cgcctggcca aagagctgat cgcccccctg cccccgcctt
     1561 cctccgcagg aaaggacgtc aacttaatgg tcgagcaagt caccaatgaa ttaaaggatt
     1621 tgtggccaaa ggaattgcag gagatccaat ctgattttga aaaaaacgaa tcgcgcatag
     1681 ggcagctggc gccgggtgat gtcaaaaaag aactgctgca ggccacgtat aaaaacaaat
     1741 acgactccct gatgcacctg cacatcctgg actccatcaa cctggaaaaa caggaactct
     1801 ccatccggca gcatgaagcg gaaatggagc gccagaaagc gcggcgttct tttatgatga
     1861 ccgggtccgg cagcaccctt ttattgtccc tgtttttatt tttaggattt acccggcaaa
     1921 aaaaacataa ccggctcctt accttaaaaa acacggaaat caaactggcg caggaacgct
     1981 ccgaagaatt gttgctcaac attctcccgg cccaggtcgc ggacgaactc aaacaatacg
     2041 gtgaatccaa agcgcaccgc ttcgacaatg taagtgtatt gtttagcgat ttcatcgact
     2101 ttacgcggat cgccgaaacc ctaccccctg aaaccctggt caaggaactg gattattgtt
     2161 ttaaagcctt tgaccgcatc atcagcaaac attccctgga gaaaataaaa accatcgggg
     2221 atgcctatat gtgcgccggg ggccttaatc caagcctggg caaccagacc tccgggatcg
     2281 tgcaggccgc cagggagatg caacaattcc tccatcagtg gaaagaggaa aaaaaagcca
     2341 ggggggagcc atttttcgag gcgcggatcg gaattcatac cgggccggtc gtagccggtg
     2401 tggtaggcat gaaaaaatac gcctacgaca tctggggaga tacggtgaat atcgcatcgc
     2461 ggatggaatt gagcagcgaa ccgggaaaaa tcaatatctc cggagaaacc ttcaaacagt
     2521 tgaatgggga gttctcatgt tcttaccggg gtaaaatcaa acctaaaaat aaggcggaaa
     2581 tcgatatgta ctttgtggag gtgagtggat aaggttatta tttcgcgcaa aggcgtcaac
     2641 cagtttaatg gttactcgca atggggcaag gcaccagtac aatccgttcc acgaggtaat
     2701 atttctcgcg gagagcgcgg gaagcacgga gaaatatttt attgaagaat gttaacctgt
     2761 tcggtcgaag tttacccagt tgggtcgaag tttagcttcg gccatcttca acttttcgcg
     2821 caaagacgca aagtcgcaaa gcgccaagtt gggtcgaact tagccatcct ccatttttcg
     2881 cgcaaagacg ccaagacgca aatcgtccaa tttagatgaa gcttggcttc ggccatccgt
     2941 tcggacgaag ctctgcttcg gccataactg aatcttcatt atctacacat attcggagct
     3001 caactcctcc cttacacaga tcttaaccgt ccaacgcatc cagtaccacc tccttcgcca
     3061 gttccttgta ccggcatagc gcgttcgcca gtttcatctg ctctattgaa tggcattccg
     3121 gttcgaaaag tgcgggggaa gccaccagga tacatatgca ttttgcccgg ctggtggcca
     3181 cattgaggcg gttgaggttg tagaggaaat tcatgcctcg tggcgcgtct tcggccgaag
     3241 aactggtcat ggagtagatc accacggggg cctcctggcc ctggaactta tccacggtac
     3301 cgatttctat cccgggtaat ttctggcgga gggcgcccac ctgggcgttg aagggagcca
     3361 caattaatat gtccttgttc atcagattat ttgttttccc ttcctcatcg gtccaaaacc
     3421 cacctgccag caatgttcca acaatggctg atatagcggc cacttcttca tctgaccggc
     3481 tctgattgcc tttgtgggga accggtacgt agaacagacc cgcttcatca aatccgcgcg
     3541 tcccgctaat ccgttgtctt tcgagtccag gtttcgaatt caatctgctc tcgtaaaaca
     3601 gctccgaagt gaacttgcag atctccggat ggaggcgcca gctcgtaccg agaaacagcc
     3661 ctttcgtccc aggtatagtc aggtggccat ccaataaatg gctgagggcg gcaatatcac
     3721 tgccttccgg atgagcccct ttctggggct gttctaattg ttgcgggtct cccaataaga
     3781 ccaggttttt agaagccctc gaagctgcaa gtgcatgagc cagggacatc tgacctgctt
     3841 cgtcgatgaa caggtaatca aggactcctt ctgatttatc ttcggaccac aaccatgcgg
     3901 tcccacatcc gattttacct tcttccaagg cggccctgac cttgtccgat tcgcccactt
     3961 cccggatgaa aggtatttct tcaggatctc caaccgaacc tttatgaacg aaaaaaattt
     4021 cgaggtcaac cggcttatgt ttgattaccg acttggccag gtttcggatc actttatgac
     4081 ttatggcagt aatcccgatt ttttttccct gtttgtataa atcgacgatc atttgagccc
     4141 ctgtaaaggt tttaccggta cccggtggtc cctggatagc cagtacactg gaatttaaac
     4201 tggtagccag cctcctggct cctgactcca aatcttcttt ttgcaataag agagggccat
     4261 catagttgta taacaagtcc gggcgcattt tggtcaataa atggaatgcc gcccggcaag
     4321 acccctgtcc attaaatcca tgatcaagca ggttccgggc aacggcggca agagagtcgg
     4381 tcagtaagcg aggatctacc cgttcaggta catgcacagc ccgtggatgc ctttctttgg
     4441 ccagacctga cttttttata tcgatggtat tgttttccag ggatatggct ttcacctggc
     4501 cgatggattt tcctttaacc tcgtccagag cgtcctcaac ttccacccgg atttcctgcg
     4561 gaggaaaacg gtaccggtgg gtcgggtttt tttccctggg acctttagga agttcttcaa
     4621 caaactccaa tccagcaatc gccttccttt cttcaagaag atcatcgtat tccagttcat
     4681 gaaccctgaa atattcccac caggcacttt tatcctctct tctgaaataa tccagcaggt
     4741 ttgccaacaa acgcctggcc agttgttcgg tatctttgct ttccgaattc aggttttcgg
     4801 tcagtgccag gaacaatttc cgggtccgtt catcgaggtc ctttgtttca tctcttgctt
     4861 ccacctcctt taacaggggc ctttcaatcg gttttcccag attgatctcc gtcttccgct
     4921 tctcttccag ccattgatgc agcgccgcag tagacctgca atcgtcttca ttgtatgcct
     4981 ccaattccgc gatcacctgt tcatccaaac tcaggaaatc attcagttca agcgccactt
     5041 ctaccctttt ccttgcgctg ctggccttat gcagttcgag tttgcgttcg taagcggcaa
     5101 atttttcgag ttctttcagg gaataggttt cgacgctggc caggagcgac tctttgaata
     5161 cctggtgcaa atcaataaat cgcaggcctc tggccaactg gtccacttcg gcctcataaa
     5221 ccgaatggat catggccaat cgttttacag tggatggctc ataaggcgca aaatgataaa
     5281 taaacatacc tggaaattcc tcccattgtg ccttcacaaa atccataaac cggctaaagg
     5341 cctgcttttc ttcatgcttg tttgaggacc aaatcctgcg gtagtccaac cccccctctt
     5401 ttttaacggc atatccaatc aggtattcca gtccccctcc ctcaaaaaag gcatcccctt
     5461 caaaatcgag atagatatcc ccttcattgg gttccggaag gcggtgaaaa cctcttccct
     5521 cttccgattc cagagtttta ctaagcagtt tttgccgtct tcgcccctcc agttgaatac
     5581 gtgcctgcac ctgccttttc aaaagtgctt cgaggttgcc cctgcctggt ttgaggataa
     5641 gatcggtttc ggcaaactgc tccagccttg aaatcccctg tgattttaat tcttcgatct
     5701 ggatggactg catacccgcc accagggaaa ggtaatcgtc catatgccgc tttttatcac
     5761 attcctgcca ccaactgcag atctgacaat gctctaccgg ctcgggataa gtcggcagat
     5821 caggaccggc catggtttct tcaaatcgtt ttttcaccag tcggtaatag gccctgtaat
     5881 ccaggtaccg gaatgcctcc cgctcaaagg gatttccccg cttgatgacc gacatgcttt
     5941 ccgcttcgcc accctgaatc ccggacaaga ggtcggtata aaggcagagt tgcaggatgg
     6001 tttccgcttt ggtgttttgc gtaagtttcg tatcctggac ttcataacaa taaggcccaa
     6061 accggctctg gccgtccact ttcaggagga tatccgcgat ccctctccag ggcccctccc
     6121 ccagtccggc ctggaccagg acctccgcac ctgatcgcat ggcttccacc acggcctcgg
     6181 agtcctttcc ccgcaaatca accacctgtt ttccctgtgc ccggaggtgc tccacatagg
     6241 ctttctcgtg ctccatcccg cgttccctca ggacatccat catcggatca tgccatcgtg
     6301 gtttcgccat ttgacccatg gccacctttc ggtttaattg ggtcaggtgg tgacaactta
     6361 ggtggttggt caggtcggtg gctgcaaggg taaaacggtc ggaatcatat ttcatagtga
     6421 tggtaaatta cgatggattc attttacttg ctggtacgtt taatttcctg attgggaatc
     6481 agtacgcggg cagcacttat tcgccttttt tattaagttt gggacttaat attcaaaatt
     6541 ttcaaagagt atgaaatata aatggctcgg acttggtctc ctcactatcg gaatacatgc
     6601 ctcccttcct tccttctcac aaggatatgt caatacgccc cctgccgtat cccctatgcc
     6661 gatgaaaccg gaaatgacgg aaatctggaa tcccgaagtt ccagtagtaa aacccggaaa
     6721 aaatctgggg gaagccccgt ccgatgcgat catcctgttt gacggcaaaa acctggacca
     6781 atgggtgagc cagcgcgatc cctccaaacc ggctccctgg aaaattgtaa acaacgacca
     6841 tatggaagtg gtgcccgggt cggggggcat ccagaccaaa atgcaattcg gggactgcca
     6901 gctgcacatc gagtggagcg cgcccgatat ggtggaaagc gggggacaag gccgcggcaa
     6961 cagcggggtc ttctttcaga accggtatga actgcaggtg ctggattctt acaacaaccg
     7021 gacctaccgc aatggccagg ccggcagcat ttataaggac cacgctcccc tggtcaacgc
     7081 catgaaaggc cctctggagt ggaatgttta cgatgtggtc tatacggccg tgcggtttaa
     7141 gcccgatggc agcgtcttct ccccagcgcg gatcaccgtt ttccacaacg gggtattgat
     7201 ccaaaacaac gtgaccatca acgggatcac cgaatacatc ggattgcata tgtataaatc
     7261 cgcccatggt gatgacgtcc tttccctcca ggaccacaat aataaaaccc agttcaggaa
     7321 tatctgggtc aggcctttgt aaaggtgaaa ggtgaaaggt gaaaggcgaa gggagaaaaa
     7381 aatgatcaag gccaccgcca cccaccccat gtaattttgc tccctttccc gtttaccctt
     7441 aaccgtttac cccttgacat ggtttgggag gagcttagct tcgaccctga aacttaacat
     7501 taggggttag cataaatacg aaaaagccga agctaagctt cgtctgaacg gccacccacc
     7561 acccaccacc caccatccac cacccaccac ccaccaccca ccacccaccg tcctgtcctc
     7621 tcatcccggc tacccgtgtc cttttgctcc ctttcccgtt tacccttaac ccttgtcctt
     7681 caatttcacc cctgccgggg gatacccctg cttttaaaat aatccacctt gcagcctgca
     7741 tttaagcgta acttagacgg catcccggta aaaaaacctc accttatgaa aaaaatatat
     7801 ttgatttcct gcctgagtct gattcttata tcggtaagcg ggccattgca aggccaatta
     7861 ttacgtcttc ctgatgccaa cgtgaccaat ctcaaggctt cggttggccg cacggtggga
     7921 gtcaccgata ttatgatccg gtacaattcg cctgccgtcc gcggacggga gggagggatc
     7981 tggggtacgg caattgtgcc ctatggatat aacgtactgg gctttggatc aaacatggaa
     8041 tccccctgga gggcaggtgc caatgagagc accacgattt ctttttccac cgacgtcacc
     8101 attcagggca aacccttaaa agccggccaa tacggtttct tcatcgccgt ttacccggat
     8161 tcctgcatcc tgatttttaa tcagaatacc aagggctggg gcagctattt ttatgacaaa
     8221 ggcctcgacg tcctgaaagt ggccacccga cagcaaaaaa acatgcccgc cagcaaagag
     8281 aggcttgaat atgctttctc caaccagacc gaccggtccg tcgaaattgc cctggaatgg
     8341 gaacgctgga ggatcccttt taaggtagaa gtggacatag acggtactac cctggcttcc
     8401 attcaaagcc agatgagcgg ggctatgggt tttgatccac ccagtcttca ggccgccgcg
     8461 gcctggtgcc tgagccacaa taccaatctc gatcaggctt atacctggat cacttccgcc
     8521 accgatccca acctgggcgg gctcaagacc ttcgccgcct tgtccaccaa agcaggcatc
     8581 ctgggcaaga tgaataaaaa ggaggaagcc gaccagatca tggcccaggc cgtggaacag
     8641 gccaatgcct tcgaactgca tgcctatggg cggcaattgc ttgcccagaa aaagatgaag
     8701 gaggctatgg atatttttga aaccaattat aaaaaacacg aaggcaaatg gccaacggcg
     8761 gtgggcatga tgcgcggata ttccgcgatg ggcaattatc cggaagcgct taaatatgcc
     8821 aaaatgggcc tcacccaggc tcccgacgac ctgaataaaa acagcctcca ggacgcagtc
     8881 aaaaagctgg aagccggtaa accgctttag gctttggaga gaaaggacaa acaccggttc
     8941 aggcaagacc tgggatgact cgctaatcaa atagtcaatg ttacatcccg gtgtatattg
     9001 aaccaccgga ttatttcaac atcaaacttt ccatgctgac atgaaacaat ggatcatgcc
     9061 cctggcattt atattcatac tgccttggtc aacattttca gtaactggcc aggaatggat
     9121 gaatctttcc ctgaactatg gcccttataa aaccggcttc cgttatattg agacttcgga
     9181 tccgggaaga ccctgcctta cctgtgcgaa atcggtaacc ggctatgccg cccgggaaat
     9241 ggagatcgcg gtttggtacc catccgaaga ccgggaaacg gccaccatgc cctatattca
     9301 atatctgagg ttaggaccgg ctggaaccat tacagattta aatattttga aacaggcttt
     9361 tttcaagagt atctccggcc tgggcgggga taccagcctt ttcgaaaccg cttttgcccc
     9421 gctctttcat gaggcaaccc gggcgcagaa ggaccctacc ctggcgccgg gccgatggcc
     9481 attgatcctc tatccggatc aacctcattt tcaaaacacc ctttgcgaat tcctggcttc
     9541 acatggttat atcgtggctt cgcccaggat taaagggact ttccgtgcgg aacctgaata
     9601 taatcctgca gggatcgaaa cgggcacgga tgacctggcc ttcaccctgg cttatatgcg
     9661 cagccatttt caggtcaacc gggaatttgc tttcatcggc accggtttca atgccaccct
     9721 cgggcttgcc ctgtctttca aaaacaaaga tttggcggcc ttggtcagcc tggagggagg
     9781 cattaccact ccctttgaag caggcctgat cttaaagagc ccatattttg atctccggca
     9841 atgctcggta ccgatgctgg tgattcacgc cccgcacccc gatgtcaaac ccgaattgac
     9901 ccaccggtat aaatattcgg agagggttta tcaatcttat ccccaatcct ccgagtttta
     9961 tttcctcaat ttcggcctct gggagaaaaa ggtccggaat attttcccca aagccaaccg
    10021 ggcaaatacc tgggaaagtt ttgaaatttc agcccaatcg gtccaactct tcctggattg
    10081 gaaattgaaa aaggatgaaa aaagtaaatc cttgctgttg gaaaacgact ggccgccgga
    10141 actgatgcaa acctcggtac gccctgccgt tccgattccc cctgaccatg aacaactttc
    10201 tgaagtatgg gaaaagaagg gcatcgattc ccttaacatg atttaccgga acctcagggc
    10261 atacgataat caaccttttg atttcagctc cttctatcaa ttgagccaga aaatgatcga
    10321 acaatcgaga ttcagggacc ttttgtcctg ggcccttctt tttgccgacg cacatccggg
    10381 atcggccatt ccttattccc tgcaaggccg cgcccacctt gaattgagca ataaagcgga
    10441 agcaaagaaa aattatgaaa aagcattatc cctgctcgga caggatcccg agctcaatgc
    10501 ggaagaaaaa aattatttcg gacaagccat tgaaacaagg atcagaagtt taaactaaac
    10561 ccgttcgaat atgaaaccgt tatttgtaat cgcagccctt gccctgtcct ttcccatcca
    10621 agcccaaagc cctgaagact ccgtagccat caaaaaggca gccctggatt atgtggaagg
    10681 cttttttaca gccgacagca gcaggctcac tcaagccatg tctcccgaat tgaccaaaag
    10741 gatcattgac catcgcagcg ggaagagcaa actcctgacc atcggtttaa aagaattgat
    10801 cgggtatacc cggatgggaa aaataccgga taaaaatccg gatgaaccgt ttaaggccaa
    10861 agtggaaatt tacgacattg ccggaggtat cgccctcgca aaggtcagca ccaacaaaat
    10921 gcctgagttt tttgattata tccaggcagg taaaatgaac ggggaatgga aaatcatcaa
    10981 cgtattgtgg gcctttaatt gaggtattca agggtttgga tcgaaccatg gcaaccttaa
    11041 gcttcctctc cggaggcttt acaaaatttt tacatccctt ttacagcttt ttaccaagat
    11101 aaagctgtgg gagggttgac tcccggtatt tttatgccat tctttcacca aaatcatttt
    11161 agccatgaaa attcatactt gtactttttt gctccttcta ctatccccct tttccggcag
    11221 aaaagaatta gaatttgcac cgaccgcccg gatcacaagc agtccgcaaa aactcaacaa
    11281 tgaaaagccg ggcctgaaaa acatcatttt taagtccacg gatggcgggc agacctggca
    11341 ggacatcagt gaaggccttc ccgaaaattt taaggaaaat ggcttctatg caaacgacca
    11401 ggggatctat ttactgtatg gtaacggggt ctatcacaga aatataaatt cccaatcccc
    11461 tttttgggaa aaagagattt tgatcaataa gcagcccaat attgccccag gtgattccag
    11521 cttatttgca tacggcaacg atggcccgtt tttacaaaaa ataaacggaa ccagggtttg
    11581 gtcgcccttg tattcaaatt ttcagggtgg agcgatccat gcccttttgg agacaaaagg
    11641 aggttcggtt ttcatctgtt ccgaccgtgg cctatataaa tccgacaaga gtggcaaaag
    11701 ctggaaacaa gtccacacag gaggctgggt gatgaaatta gcagagtcaa acggtgtact
    11761 ccttgcaacc agccaggggg ggatattaag atcgaccgat gagggtgaac attgggattt
    11821 ggtgatcagc gagggcggtg tgggcatcgc cgtggaatgc atcaacggcg gattcgccgc
    11881 catcacctac aatacggaat cgaagaccag aagagtgcga atatcctacg acggcgggaa
    11941 aacctggtcg cccattgacg ccggccttcc gccaagcctt tccatagctt ccataattca
    12001 ggctggcgaa aacttctatt gtggtcatcc cgacggcatt ttcagatcat cggaccaggg
    12061 aaaatcatgg agtcttatgc ttccttctgt cgaaaataag gtattcaatt taacagtttc
    12121 aggcaaagtg atttatgcca tacccaggga tggtggttgt taaatccccg atttcccagg
    12181 cacctacaac ctgcgtcctt atttattatc caggtttttc agctcctgat cgatcggata
    12241 taaataagga tagtcctcaa catattttgg ccatatgtat tttccatgcg gaataaaaat
    12301 cccaacaggg atttgtttta ctttaagttc ttcaaagtcc tgttcagtca tggtaaagtg
    12361 ataggccgtg atgccttcat tataaaacat cacgtctata tcttcaaatt cattcatatt
    12421 caccaccgtc tttacatttg gcggaagcag acgatttaat gccttgtaaa tttcggtatt
    12481 gtatatcctg atgtttcgtt cattgttttg gggtgacaga tagtttatga accagctcgg
    12541 atttaagctt aaatagactg ttgccaggac cagggcagcc aggaccagcc gggattttcc
    12601 caatttctca aaaatataat ttaaacccaa cgaaatatag atcaaaccca gcggcacaat
    12661 aaaaaaaaca taggtatcca ctttggtctt cacgaacacg ctgaaaaacc agaaaacaaa
    12721 taacattccc gcgatgatcc ctgcatttaa ccgtccggaa atttcctttt taataaaact
    12781 caacaataaa ccaacaggaa tgaaataata taaatatttt cctattaaat ccacaagatg
    12841 gtccagataa aaagaattgt ccccggtatg gccttccagg gcttccgtaa tatgcctttt
    12901 attaaaatca tagacaaaat ttgccaaatc aggaaaccgg tccaggatat atatttgcca
    12961 gggaaggaac accgggaaac aaaaaaacaa ggaaaggaac gtatgaatga cttgttttcc
    13021 tacactcgat ttttgcaagc cggtgatcag aataaccact ccccagatca aatacaccaa
    13081 taatccggtc agccacttat tcaaaatagc ggcgccggaa aaaacaccgg tcaacacaac
    13141 ccagtaccat ttctgattcc ggtgatattc acagtaagtc caaatactgg caagaacata
    13201 atatccgtgc gccacatcat tatgatccat cccatgcatt ccggcaatca actgcaaatg
    13261 aaatccggaa acggccagca ttaaagcgga gaataaggcc accgtcctat tttttgtcag
    13321 gaggatcgct atcctgtaag taagaaaaat caagatcgtt cccataacgg cgctgggaag
    13381 acgcatggac cactccgata ccccaaaaac tttcatactc aaagccatct gccacataaa
    13441 aagaggctgt ttgtgcagcc agacatgatt gcagcaccag ataaacttgt catagttgtc
    13501 tgtgacagga tttgcccgta gaacaggaat caagggatgg tccatgagat tccgggccac
    13561 cagggcgtga aaccgctcat cccagggatg gagaaatgga tctgtaaaaa tggcggtaag
    13621 ccttaaaaca aatccgatta aaagtataat gaataaggtg atgttctttt taatatccag
    13681 catcgtattt gttcatagtc tctcacttat ttcagccggg tgaaagcggc ggaagatatt
    13741 gaatatggaa tagagaatcc gctacctcct atctctgcat agacccaggg acctctcccg
    13801 aagcctaagc ctcctacctc ttacctccta cttctactcc aatgtcctca acttaatatt
    13861 cctgaaccac acctcatccc cgtggtcctg cagcaggata tgtcctttgg ctgcggaagc
    13921 aaagcccggg tatactgcat acttgctcct ggccaccagg gccttgaaaa tattgctgtt
    13981 gcgttcgtat tcgaccacct tgaagccatt gagccagtgc tgaaccatat tgtttggata
    14041 aacgattatc cttccctggt tccaatcccc gattttccgc tgaaaacggg aatttaaggg
    14101 agtggcgggt atcaagtcat ataaactgga cagggtccgg ttgcccacta ctccctgttt
    14161 tgcatccgga tgacgcgcat cgtccaggac ctggtattcc agcccgatcc cggacctgcc
    14221 cccggagtcg tattgttcgt ctacaaaata tttaagcccg gaattggctc cctcggtcag
    14281 tttaaaatca aaggtgagtt caaatgcccc gtattggtcc gtggtcacga tgtcgttgcc
    14341 cgtctccgaa ccgtccgatt tcatcacccc gatcatgcct tccttcaccg accaccgttt
    14401 ttccgggggg ccggtttggt gcactttgcg ccagccgttg aaatcgttcc cgttgaacaa
    14461 taatttgaat ccctggtttt tttccatctc actcaaatgg ttgttcatca ggttaacgac
    14521 cggggtttgg tcatagggcc gcggtttgag acccgaggtc tggatacgga tattcctcca
    14581 ccggatttca gtgccgggag cctggtcctt gccaatgctg tgcacctgga gggcgataaa
    14641 acctttgagg gtcaggtcgt ccaccaggca ggccaccggg atatcgttga tccaggtgcg
    14701 gatcaccggg cctatcgcct caatccggta tttgttccac tggtcctttt taaaagcctt
    14761 tttgccttcc ggattgatgt ccgggatata gagccagtct ctgcgcgcct cgtcgtagat
    14821 cccacccgac caggcccggt cggacggatc gatttccacc tggtagccgt ggacccgtcc
    14881 ttctttatag gagggaaggg aaaggctcct gatctggatc ccggagttca tgctgttatc
    14941 cacttttagt tcgagctcga ggataaaatc accgtattct atttcggtag ccaggaagga
    15001 gttgggggta ttggcgaccg tggtccccac catctggtta ttttcaacgc ggtaccgggc
    15061 ctgtccgttt aattgtttcc aacccttcag gcttttcccg tcgaacaggg aggtccaggt
    15121 ttgactttga gccaaagagg ataagaacag aaggaggatc catgcatgat tctttttcat
    15181 atccggaatt ttttgctgaa caagataaaa agcttttcgc tgcctgcttc caaaaccgca
    15241 gaggagacca ggaattccgg aaaccttagc caataaatac acttaatttt atgttataga
    15301 cccatatttt ttcccggatg aatgttttgt ccccatcacc tctccccact accgatcccg
    15361 aacccaccga tccccgcagg ctggccggga tgctgcgcca aaacctggat ccctaccccg
    15421 gcccctggga tgaaaagggg ctcaaacatc tccttcgccg cagcctcttt ggcacctcag
    15481 cagccgaagt cgaacaattt ctcctgctgg gaaaccttga aaacaccctc tctgccctat
    15541 gcgagatcaa acctcctgcc agcctccccc tggtcaatta taccgatggg gatggggtcc
    15601 gggagccaaa cgccaaagac ggcgaaacct ggatccatga agaatacaac aatgagctcg
    15661 aatattaccg cctgctctcc ctaaaaacct ggtggctgga cctgatgctg aaagaccaga
    15721 cgctgatgga aaaaatggtg atcttctggc acaaccatat cccggtggaa atggtagggg
    15781 tctttcacgc gcgccaggct tacgactacc tggccacgat ccggcaatac gcccttggta
    15841 atttcaggca attgatcagg gcccttaccc ttgatcccat gatgctgttt tatctcaacg
    15901 ggaattcaaa caacaaatcg gcgccggatg aaaactatgc ccgggagctt caggaattgt
    15961 tctgtatcgg caaggggccg gaagccaaat tcaccgaaga cgacgtgaag gcggccgccc
    16021 gcgtgctcac cggctggaaa tcggactatc tgaaaccgaa aacctttttc gcggaatggg
    16081 cccatgatac cggggataaa acattttctg atttctacgg gggaagagtg atccgcggtc
    16141 agaagggatc agaaggccaa aaggagctgg atgaactgat tgatatgttg atcagccacc
    16201 cggaatgtgc caggttcatt tgcaggaaac tgtaccggtt ctttgtttac cctgaaattt
    16261 cagactggag tgaagaaaac atcatccggc ccctggcgga tcttttcata aaaactgatt
    16321 ttgaaatcgc cccggtcgta aaggccctgc tgtccagcaa tcattttttc gatcccctgc
    16381 tccggctggc catgatcaaa tccccgctgg attataccat aggactgaac cggcattttg
    16441 gcatgcggtt cccctcctcc cccggagaga taaaatccaa attccaggcg cagacggtga
    16501 tgtattattt tctcacttct tttcagcagg atatcgggga ccctcctaat gtttcgggtt
    16561 ggccggctta ttatcaattc cctcaatacg accgttcctg gatcagcacc cataccgtcc
    16621 accagcgcgg gatccattcg gacatgctgg tctggtatgg ctatcagatg gaccagggcg
    16681 tggctaccat agactggccg gcctacaccg acaccctgat ccatccggaa aatcccaacc
    16741 tcctgatcga agaagtgctt ggcaggctgt atgatgtccc ggtggatgcg gaggtcaaaa
    16801 aatatctcaa aagcatcctg ctctccgggc aggtcagcga ccattactgg accaacgcct
    16861 ggcaggaatg gaaatcgaaa cccaatgatc ctatggccag gaatacggta gatatccgtt
    16921 tgaaaatttt ttaccaatac atcctgcaaa tggatgaata tcaaatcatg taaggggtat
    16981 gaaaagaagg gattttttcc ggttgacggg atcaggcctt gcggctaaca gttttttacc
    17041 gggcttcaga ccggataaaa ttcctcagtg gtttgactgg ttcaaactgg atacggaccg
    17101 ggtcctggtt ctcatacagc tcgatggcgg aaatgatggt ctcaataccg tgatcccgct
    17161 cgaccagatg gacctgtaca acaaagcgcg ccaatccgtg gccctgcctg ccaacaaggt
    17221 tttgtcactc ccaacgacca ttaaatcagg ccttcacccc gctttggatg aaatcagggg
    17281 cctttatacg gaaggcagga taaagatcat ccagtccgtg ggttatcctt cgcccaatta
    17341 ttcccatttc cggtccaccg atatctggat gtcggcctcg gatgcggaag aagtgctgag
    17401 ttccggctgg gccggcaggt acctgaacca tgaatacccg aattatccca atggttttcc
    17461 caccgaggac atgccggatc cgctgtccgt ggagatcggt tatggccaga gcctggcctt
    17521 ccaggggccc gccaccagta tgagcgtcac catagcaaac ccggaggact tcaataaact
    17581 gatccagggc atcgagacac ctttgcctca aacacctttt ggagacaaac tgggttatgt
    17641 ccgtttaata aaaaaacaat ccaataccta cggcgaacga atgaaggacg ccttcgggaa
    17701 aacgaaaaac aaagccagtt atccggagga caacgacctg gccgatcagt tgaagatagt
    17761 ggcccgcctg attgccggcg gactgaagac ccgtatctat aaagtaagca tcagcggctt
    17821 tgacacccac gacaaccagg tgcaaaacgg ggatcacgca accggtgaac atgccaatct
    17881 tctccggcgg atatccggtg ccgtgaaagc cttccttgac gacctgaccg ccctcggcct
    17941 tcaggaccga gtagtgggca tgacggtttc cgaattcggc cgacgcatta tatccaatgc
    18001 cagccgcggt acggatcatg gagccgccgc gcccatgttc cttttcggca atgccatcca
    18061 gggcggcctg ctcggggaaa accccaccct gcccgcagaa gctaaatggg acgacaatat
    18121 ccccatgcaa tacgatttcc ggtctgtcta tggcagtttg ctggaaaact ggttttgtat
    18181 caataaaacg gaggtacaac aagtcctttt aaaaaattac cagaccctgc cggttataaa
    18241 gcccgcttac agttgtcttt ccaccccgac ccgggaagaa aatcagttaa aaggaatcag
    18301 catcatccat gcttttccta atcccttcac ctccatcata aattttaaag ttcaatccaa
    18361 cggcgaacat tccagtattt ccattttcaa tccggccggc caggtgatcc acaacataca
    18421 tcatggcctt ttatcaaaag gggaacatca gtattactgg aattcggaag acctgccggt
    18481 aggaaattat tatgtgcggt ttcaatcagg gcctgagcaa caggtgaagc tgattgtcaa
    18541 agtgagatag gaagctggaa gctgggagct gggagctgga agctggaagc tgggagctgg
    18601 aagctggaag ctggggcgct cgcgtaaacc atacagcagc tacgccaaaa gaataaaggg
    18661 accgactgac tttgaattca cagaaatgcc ggacacaagg ccccctaact tctaacttcc
    18721 gggagatgga agctgggagc tggggcgctc gcgcaaatca cccacagcta cgccaatgga
    18781 acatagggac ctactgagtt tgaatgcgca ggaatgccgg acacaaggcc tcccctagct
    18841 tctatcttcc agcttctaac ttctgattgc tgattgctga aagctgaaag ctgattgctg
    18901 aaagctgaaa gctgaactct caaagctgaa atctcttctc cagcactttc tggatatcct
    18961 catattccag cagaaatcca tcgtgcccat aatcggaatg gatcttgtcc agggtggctc
    19021 cggggatctg atcggcgagt aatttttgtt ccgcgagggg gaaaagcaca tcggtgttga
    19081 taccgatgac cgccgttttg gctttgacca gggaaagggc atgaggaatg cctccgcgct
    19141 tgcggccgac gtgatgactg tccatggcct tgctcagggt gatgtaagaa taggcattga
    19201 agcgacggct cagcttcagt ccctggtacc gctggtagga cgatgccctg aaatcatcga
    19261 ttttatcttc ggtctcgtat tgtttgagat caaaagtgat gtaatggcgg taggaaagga
    19321 tggccaggct gcgggctgct tcgagcccga gcgggttgac attgccttcc gcgtctatgc
    19381 cggcttcgat ggccatacgc tgcgcttcgt tgaaagcgat cccccaggga gaatgcctcg
    19441 cgttgcaggc cagaaggcag atgcgttcaa acaggtaagg atccgtaatg gcccattcga
    19501 ggacctgctg tccccccatg gaccccccta tggagaggaa gatatgatca atccccagat
    19561 gtttttgcaa taaacgatgg gcatggacca tatcgcgaat ggtcaccagg ggaaaggagg
    19621 tgccataggc ttttccggtc agaggatcga tggaggcagg gcccgtggtc ccgtaacagg
    19681 atccgaggat attggcacat acaatccggt atttctccgg atcaaacaag cgcccggtac
    19741 cgaccagtcc cggccaccat tcggagggat cggcattggc agtgagggca tggcagatcc
    19801 agaccacggg cttgttctgc ccaccggtat cggtataagt aatatcaagt ccggctaagg
    19861 tggcgccccc ttccatgtag aaaggcaggc cggttggata gactttattt tcagccacgg
    19921 taaaaggcaa aaatataaat caattagctt tgcacccaat tttataaaag cggatgaaga
    19981 ttacactgag gagaatcggc gatccccttc attttgaagc cgccaatgcc cagggcaata
    20041 ccattgaaat ggatgccaag gctgcttccg gaggcaccgg aaaaggcatg gggcccatgg
    20101 agaccctgat cagtgccctg ggaggatgca gttgcatcga tatcgttttg tttttgaaaa
    20161 aattccggca ggaactggcg gatatccggg tggagatcga agcggaccgg gatcccgatc
    20221 atattccctc cctttttgtg aaaattcacg cccattacca tttgtatgga aaactggatg
    20281 cccgccaggt ggagaaagcc atatctcttt ccatcgataa gtattgctcg gtatcccaga
    20341 tcctgaaaaa atcagtgccc gttacctatg cgttcaccat ccattcttta tgagttcaag
    20401 aaaatttaaa gacgaaacca ttgccatccg gacccagacg gaacgcaccc ggcatgggga
    20461 gcacagcacc cctcttttcc tgacgtccag ctacgccttt gattccggcg atgagatggc
    20521 cgatgctttt gccggcatta cggaagccaa tgtctattcg aggtacacca atcctaatgt
    20581 ggaggaattg gtcaaaaagt tctgcctgct ggaagaagcc tcggcgggac tggctacggc
    20641 ttccggcatg gccgcggtgt tttcttccat tgccggcttg ttgaaccagg gcgatcacct
    20701 catcgcttcc agggccttgt ttggatcttc catccaggta ttgagccata ttttaccccg
    20761 gtggggcatc acctgcacct atgtcgatcc ggtcgatctc tccggctggg aacaagccct
    20821 gacccccaat acacggatgg tcttcctcga aaccccttct aatccatccc tttggatcac
    20881 tgacctggct ggggcaggag ccttcgcgca cgaccacaac ctgatccttg cagtggacaa
    20941 ttgttttgct tccccggttc ttcaaaaacc tatgaaattc ggtgcctccc tggtcatcca
    21001 ttccgctacc aaatatgccg acgggcatgg acgcgtcttg ggcggcatcg ttgcgggtaa
    21061 agaggacttg atggaaaaga tcgctttttt caacaggcat tccggcccct ccatctcccc
    21121 ttttaacgcc tgggtgatca gtaaatccat ggaaaccctg gccctgcggg taagagccca
    21181 ttgcaagcag gccgaagaac tggcttcccg gcttcaggat caccccggac tggaggcggt
    21241 gatctatccg ttttcagaaa accacccgca agtcgacctg gccaggcgtc aaatggaagc
    21301 aggtggcgga atcgtttgca tcaaggtaaa gggagattat gcaaagacgc tccaatttat
    21361 caaccacctg gaaatgattt ccatttctcc caacctggga gacacccggt ccatcgccac
    21421 ccaccccact tcttccaccc acagccgcat gacggaggag gataggttga cggtgggcat
    21481 cacccccaat ctcgtccgct tcagcgtggg gctcgaacat attgacgata ttgaggagga
    21541 tatccggatt gccttatcca aaatttaaat cgtggataca tgaaaaaagg acgtaatctg
    21601 ctgtggttgg ccttttggtc ctgccttctg ttttatcaat gcgggccggc gggggatgaa
    21661 aaggatcccc aatacctggc cgacctggcc attaaagccc acggcagcaa ggttttcgac
    21721 caatccatca ttgaatttaa attccgtgac cggtattata aatccacccg caaggacgga
    21781 aaattcatct atgagcggtt ctgggactcc gtaggaaccg taatccatga tgtactcgac
    21841 aaccggggct ttagccggat cgtggatcag accgaagtga tcctggacgc caaaaagaaa
    21901 gaagcctttt ccaattccct gcgcggggtc ttctatttct ttggcctccc ctttcagctc
    21961 aacgacccgc ccgtgatcaa agaatacctt ggggaagtga tgctcatgga acaaccctat
    22021 cataaactga agatccgttt taagccggac ccggccgacc agctcatcca tgatgacgtt
    22081 tatgtcttct ggatccataa gaagaaccat accgtggatt acctggctta ttcttatacc
    22141 gagcccaatg aacgaggctt acgttttcgg caagccatca atcccagaaa aatcgaaggc
    22201 ctgagggtac aggattatat caactacaaa ccgatccggg acagcagttc cctgaaggtc
    22261 gaagacatgg atatggcctg ggctggcaat caactcaccg aaatgtcccg gatcatcaat
    22321 gagaatgtca aaatccgcct gaataaaaac tgatcccctg ccctgttttg ctcgcggata
    22381 cccttgatat tcaaaggctg tgccttataa aattgttgta tttttgattc actttcacaa
    22441 tcagccgcaa tgagggaaaa attattattt accacggtct ttgtttggtt aagttgccag
    22501 ttcgttttcg cacagaaaat ctccatcggc ctggccagcg ggaaggacgc aatcaacgaa
    22561 aaatgcggcc tgctgaacaa cgaccagttg gtattcaccc tcgacagtgc ggccaaacaa
    22621 aatacccggt ttgtaaccaa tctgggcgga accgcgatac gaggcacgga ttatgccacc
    22681 aacctgacgg ataccgtgct ttttaccacg ggccagcaaa gccgcagttt tgacctgacg
    22741 gtattcgggg atgcattcgc ggaaagtgaa gaaaccgtgg tggtgatcgc aaccggccag
    22801 gatggctttg cagatacttt tctcatcacc atccaggacc gtattgcccg gatcctgacc
    22861 tcccaggata ccttccgtca gtgttcgaac gaaccattta aattgaattt ataccgtgcc
    22921 gccgggtccg tgatcaattg gtttcccgcc gacatggccg ctccaacgga tgacccgaat
    22981 gttttcacca tcctcccggt ccggaccagt ccggtgatcc tgaccgccac catcgaccaa
    23041 tgcgtcgaac gcgacacgat ccaggtgatc agtcaaccta taggggtcac cctaaatacc
    23101 aaagacacct tatttctttg ttttccggat tcattcaggc tggtggccac cctgaccccg
    23161 cccaatgccc agatctcctg gagtccgctc gacagtacga cccgggtgat caatgcttcc
    23221 tcaatccagg tcaaaccgcc aaaatccgcg cgttattttg tgaatgcggt ttcaggcgtt
    23281 tgtaaggcag cggatactgt gtatgtgagc atggactcgc tgcgcgatac caaaatcatc
    23341 cacttcccgg tgaaggataa atattgtccc ggcgacagca tctttttctt cagccagcgg
    23401 catcccaagg acctgttccc ggccctgaag ccgatgtggg acccctccaa cgggtttcag
    23461 acgccttcgg acacttttaa tgccgtagtc agcgctgata aaacaacgac ctatttgagg
    23521 actacccgga acaatgcctg cgtccaccgc gacagtgttt atatcaaggt ggtggaaccg
    23581 accatacccg ttgggccggt ggatacgacc gtttgtcccg gagaaactgt ccagatcaat
    23641 ttcaacaaag acagttccgt ctatgaagat tttaaatggt cgccccagga tggactgatc
    23701 agttgtgaaa aatgcgacaa cccgaaaatc ctcgtgcttg gccagcaaca atacatggtc
    23761 gaggcaaaaa aagatggttg cgatgccacg gccatgatct ccaccagcgt gtttcagaag
    23821 ccgcaggttg gggtgtttac caatatcccc caacccatcc ccgccggttc gaatgtgcaa
    23881 ttcctgctcc agctgcccac agggaccaaa gcctttaaat ggagtgtgga ccggaatgct
    23941 gtaccctccg gcaacctgac cactacggtc aacaatatcg gtcccggggt ccatgaagtg
    24001 gatgtggaaa tcacggatgc caacggctgc acctggtctt accagttcct ggtcgaggtg
    24061 ctttgcccac ccaatgccct ggccttgcag cgcagccccc agggcaacat ttatgaaaga
    24121 agcactttgc agatcagcgc caccggcatc aacagcaacg tcaccaatgt gcgctggagc
    24181 gtcaacggca gcaacctcaa tgaaaacggc cttacgctca cccacacccc ttccacagcc
    24241 ggcaattaca tctaccggtt tgaagccatc gatctcaacg gctgccccct ggccgccagc
    24301 atcactgtac aggtcattcc ctgcatctcc cccgaggaac tgaaaaagaa gattccgaat
    24361 gccttctcac ccaataacga tgaaaaaaac gattatttca attactcgga tggcacgctg
    24421 accattacca aattgttggt ctttagccgc tggggccaat tggtgtataa caataccgat
    24481 cctgaaaacg gatgggacgg ccgggtcaat ggcaatgagg ccgcttccga tgtttacatt
    24541 taccggctca cttatatctg cggggacgcc gcaccgcagg aggtgagcgg taccgtgacc
    24601 ttattgagat gattccatcc accatccacc acccaccaat atgaaatctt tcctccccat
    24661 cctacccctc ctgttattct tcggatgtgg tcagggccgt caatcccaaa ctaacctgcc
    24721 tggaaaagca gagaccgcac ccatgcgcga agtggtgctc atgcccaaaa ccgaagcggg
    24781 tttgcggctt atggcggatg aactggccca taaatacctc atcttcgacg gccatgtgga
    24841 cctgccctac cggctgaaca taaaaaattt ccggcctaca aaggaattta taggcatccc
    24901 cgtggaatca aaggaagggg actttgatta tgtaagagcc aagaaaggcg gtctgagcgc
    24961 acccatcatg tccatctata tccccgcctc ttatcaaaaa gggggtgcaa aaagttttgc
    25021 cgaagaactg atcgacatgg tcaacagcat catcgagggc catccggaca aattcgcccc
    25081 cgggcttacc cccgaccagg tcgaagccaa ttttaaaaaa gggctgatat ccctccccat
    25141 ggggatggaa aacggtgcac ccatagaaaa cgatctccgc aacgtcgccc atttttacga
    25201 tcgggggatc cgctatatca ccctcaccca ttccgaggac aatcagatct gcgatgctgc
    25261 atatgcttcc acccgcacct ggaaagggct cagtccattt ggagtagagg tggtgaaaga
    25321 gatgaaccgg gtcggcatgc tcgtggacgt atcccatatt acggacagca gcttctggca
    25381 ggtcctccgc gtcagccggg cgccggtcat cgcctctcat tcctcctgca ggcattttac
    25441 ccctggtttt gaacgcaaca tgtccgatga catgattgcc gcgctgaagg gcaacggggg
    25501 catgatcatg atcaatttcg gatccgattt cctcgacaat gaaatccgtt taaaaaacaa
    25561 ccgggaccgc aatgagctgg aggctctgct tgccgcacaa aatctgagcc accgcgacag
    25621 cgccgcgaaa ccgattataa aacaattcga ggccagccac cccagccttt attcagatgt
    25681 aaaaagagtt gcggatcaca tcgaccatgc ggtaaaactc gccggcatcg atcatgtggg
    25741 ttttggctcc gattacgacg gggtgggcga ttccctgccg gtcggcttga aagacgtctc
    25801 cgcctatccg aatatcattt atgaattgct ttcccgcggt tattcggaag aagatatcgc
    25861 aaaaatttgt tctgcaaatt tcctgagggt ctggagaaaa gcgattgagg tagccggcaa
    25921 ctaaatccag ccttcccgcc ctagcctttg ccttaacctt aaccttaacc ttaaccttaa
    25981 ccttaacctt aaccttaacc tttgccttaa cctttacctt aacctttgcc ttagcctttc
    26041 tccttttaat gtttatttat atttttatgt aaaacctgtt cctnnnnnnn nnnnnnnnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26161 nnnnnnnnnn nnnnnnnnnn nnnccttagc ctttctcctt ttaatgttta tttatatttt
    26221 tatgtaaaac ctgttcctat gaagctgagg ttattattca tggttttacc ggccctgacc
    26281 tggtttgcct gtcagcctgc ccagcccacc ggtcccatcg tggagaaagg aaaagatttt
    26341 gattcgtact ggcaccaggg caaagcggaa atcacttcct acgatttatt tcaggcccgt
    26401 tatggtgaaa tgagggaagg cgaagccgtg atcgtttttg tgaccgaaga gcatgaccgg
    26461 gaaaaacagg tcaaactgga ccagcccggc atcgacccgg ccctgaaagt cggggtctta
    26521 aaaatgaacc tgaccaaacg cttttatacc ggtctttatc cctaccacat gatgctctcc
    26581 agttttgtgg aggccggagg cattgccccc tggcagggcc ttaaagtggc cgcatcctgc
    26641 atggaatggt gcgggcagac ctatgcccag ctcaaccggc gaaatgacca ttaccagtac
    26701 cagctccatt cctattttga aaaagaaaac caaatcgaca aacaccttaa ccgccacatg
    26761 acggaagacg ccgtctggaa cctgatccgg atcaacccca atgccctgat ccagaccgac
    26821 agcatcctga tcatccccag cctgctgttt gcacgcctga gccatatcga actgaagcca
    26881 taccgggcgc ggacctctca tctcagcctg gacctgggca ataaagttta tcacattgaa
    26941 tatccatccc tcggccgttc actttatatc gaatacgagc ctgaattccc tcaccagatc
    27001 gtgcgttgga gtgaagaata taaagacggt ttcggtgaaa atgccaaagt attgaccacc
    27061 acggcgcggc gcaaaaaaca gatcatgtcg gattactgga accggcacgg catagtggat
    27121 gacagcctgc gaatggagct cgacctgatg aaattataag cctttgggct gtgggtggct
    27181 gattatcttt ggccctccaa gcaaagcgca gtcatgaata tattccaggc catcctgctc
    27241 gccatcgtcg aaggcattac tgaattcctt cccatttcct cgacgggcca tatgatcatc
    27301 gccagttcgg ccatgggcat ccataccggg gctttcacga aattgtttga agtggcgatc
    27361 caactgggag ccatccttgc cgtcctggtc ctctatcgga aaaggttcct tcagtggacc
    27421 aattaccagt tttatttaaa actggccgtt ggggtcttgc cggccctggt attgggtttt
    27481 ctgttttcgg atcagatcga tgccatgctg gagagccctt tcgtggtggc tttatcgctc
    27541 ctcctgggag gcattttcct cctctttatc gattccgttt tcagtcatca caccatccat
    27601 caggaagggg agatcggata caaaaatgcc ctgatcatcg gattctggca gaccctggcc
    27661 atgatccccg gggtgagtcg cagcgcggct tccatcatcg gaggcatgca gcagaaactg
    27721 gaccgggacc tggccgccga gttttccttt tttcttgccg tacccaccat ggcggcggcc
    27781 acgctctatt ccctcttcct caaatcctgg atggtcgacg gtgtcgaaca aaaaggattt
    27841 gagatcatta cgggcactac gaacaatacc cttgtttttc ttaccggcaa tgtggtggct
    27901 ttcatcgtcg ccatgctggc catccgcttt tttatcggtt atctccagaa gcatggattt
    27961 aagctttttg ggtattaccg catcctggtc ggagtcatct tgcttatttt gatcaaggcc
    28021 aataaattat aagttccccc aaccacacca cgaccgaaaa aattaataat taaataatta
    28081 acaatcaata ctttacgttc ctattgccct ccgtggcctt cgtgggctcc gtgtgcaacc
    28141 aatccgaacc ctgggaggag cgtagctcga ccttttaact tgttcgggag gagcggaact
    28201 tcgaccaaaa ggatcccaca aagacaccaa gttaaaaaaa ttaataatta aataattaac
    28261 aatcaatact ttacattctt tgtgccctcc gtggnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28381 nnnnnnnnnn nnnnagctcc gaccaatact ttgtggccct tgtgcccttc gtgggaaact
    28441 ctctgtgccc cgttgtggga aaccctaccc tgggaggagc gtagctccga cccaccagtt
    28501 tcccggaaaa accctaaatt aattgctcca accgcccgcc atgaaaagtc ctaaaactct
    28561 tgtcttcctc agcttactga gctgctccca accttcaaaa actgatcccc ggtccctggc
    28621 caaagaaatc gaatccgccg acctcgccat gagcaagctt gccggcgata tcggtttcaa
    28681 caatgccgtc cttcaatacg cggactcctc gatcgtgaaa ttaagcgaag gaaaaccacc
    28741 catcgaagga atcaccgcct tctccaaatc ttatgatcac gaaaaggata tcaaaaccat
    28801 atcctggacc ccatcccatg cagaggtagc ccaatccggt gagctcggct acacctgggg
    28861 gacctggacc ctgaatctgc cggacacggt ctttttggga aaatatttta ccgtatggaa
    28921 aaagaatgcc cggggggaat ggaaggtggt gctggacgga ggaaacggca ctaacccacc
    28981 tccaaactaa gcatccccca actgccattt tgactttaca aatacccttt gcacccagct
    29041 acaaacggtt acttccggat acaatcagct acaatcatct atatccattt gtacacggga
    29101 aatcgaagga tttccgttcg gaaaacggtg gatattcctt tgttatattt cggtggaaac
    29161 tctaaattag ctttatactt atttgatagt taatgagaat atctgactcc cctctaaagg
    29221 accaggcctt tgaatttacg ctttcaaaac gcaatgattt atagatgcct gggacatatg
    29281 ggttcactta ataaattcct gtaaattgag attccgcagc cgaatagcca aaatgattaa
    29341 accaaaagcg tactcattta aagctgatat aaggaaatat acgcaataaa catgcgtaaa
    29401 taaagatgtt agtggcaagg ctatgacgac acaacccgtc agacaaaata gggttctttt
    29461 tgacatttta tatttaagca gttgcttaaa taaacaaatt gactaccttt gtgctatgga
    29521 caacaattct tgtataagac aacaagcgga cattaaacaa ataaaccgct gtaaagaccg
    29581 agtttcagaa ctcaacggct cgtttgacta tttatcgaac ggacttgaat t
//
LOCUS       DAOQXT010000054        29456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1856_length_29456_cov_5.598449, whole genome shotgun sequence.
ACCESSION   DAOQXT010000054 DAOQXT010000000
VERSION     DAOQXT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 29456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29456
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1856_length_29456_cov_5.598449"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="PKM27_10695"
     CDS             <1..1603
                     /locus_tag="PKM27_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR07771.1"
                     /translation="EIIDKSMYPGATIEDKLGKMINSDFLNGLEVLYAIDKISSRIET
                     LGIGKKQINYKMRDANYSRQRYWGEPFPIIYDPEGIPHTLPVEELPLTLPPLDDFRPG
                     SNGKAPLSKLESWVKLPNGYTRETDTMPGFAGSSWYFLRYMDPHNDLEFASASSIRYW
                     QDVDLYVGGTEHAVGHLLYSRFWHKFLFDLGYTVTKEPYKKLLNQGKIQGISESAFLL
                     KEKQGGRHVFVSRTLMGPDDHKDCIALPVPIEFVKDYGSMNAYLDAGGIRQFIRFMPD
                     YGEALFKTEQGIYTSENFPPEGRFYTQAELAKMSKSLHNVINPDEVVAEYGADCFRLF
                     EMFLGPLEQSKPWDTRSIDGVGKFVRRIWSMFFNEGKFEVSGEPATAEELKILHRCIK
                     RVTEDIERFSFNTCVSHFMIASNELKAVHCNKREILDPFIRLMAPFAPFLTEELWERL
                     GHGESIHLTGYPEADPAHLFESVVNYPICINGKKRGNADYPADADPASLESMTLGLDF
                     VQKWLEGKTPKKVIVVTGKMINIVL"
     gene            1647..2234
                     /gene="rdgB"
                     /locus_tag="PKM27_10700"
     CDS             1647..2234
                     /gene="rdgB"
                     /locus_tag="PKM27_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNR07772.1"
                     /translation="MPPSTLLFASQNRKKAEEIRAILGDAFQIRTLAEMGYEQDLDEP
                     FDTVEANAKHKARQGFELFKLPCFAEDTGLVVEALNGLPGVQSARYAGKNKDPEANME
                     KVLSEMKGITNRRAYFIAFIAYYDGLEFFLFVGRIHGLILHEKHGNGGFGYDPIFQPD
                     GFERSFAEMDSSIKNRISHRAGALHGFTSWLKGKG"
     gene            complement(2289..3149)
                     /locus_tag="PKM27_10705"
     CDS             complement(2289..3149)
                     /locus_tag="PKM27_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNR07773.1"
                     /translation="MKSYLSLIKFSHTVFALPFAAVGTVLAFKAGYALDWLLGLKILL
                     CMVFARSAAMAFNRWADERYDRLNPRTAGREIPAGRVTSHEALWMTLISSLAFVVTTW
                     SINSICFYLSPLALLTILGYSYTKRWTAICHLILGGGLALAPIGSYLAVTGIFSLIPL
                     LYSGVVFYWVSGFDIIYALQDEEFDKVHRLHSIPARFGKSKALKISRLLHFISAMILF
                     FASWLIHNRYESSGVWLWLSALVFVSMLIYQQNLVKPGDLSKINLAFFTTNGLASVIY
                     GVLFSMDLLF"
     gene            complement(3158..5542)
                     /locus_tag="PKM27_10710"
     CDS             complement(3158..5542)
                     /locus_tag="PKM27_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNR07774.1"
                     /translation="MILVRPEALFQHLEFDKLNHSVAALCLIEDTRSRYFPMAVMTSK
                     QAIDLALDETLEFKSAYERNSLLPVQACPSISESLKRTDIQDYVFDLEALVHLFQVVK
                     NLEEIALFFRNPDQVKAMPLSIRQFERLNYTPDLYREMRTIFDDSGQIRPDATPELKI
                     ISRAIRSKEEEIDQVFRSLLNEFRKQGWLSESGESVRNNRRVVAVQSEYKRRIKGIIH
                     DESTTGRTTFIEPERIIEKNNELFDLEMDYQKEIYRILQALSKSCRQHADMLRMGWEM
                     LLHFDFIRSKAIFAVNCQAYRPEVTDRPQLKIKAGRHPLLFLKNKGLGRETVPFDLIL
                     TGDHRVLILSGPNAGGKSVAMKAVGLIQLMAQSGMLIPAHEASEMGVFHHFLGDIGDQ
                     QSLEDDLSTYSSRLLKMKQFLEVADDRSLVLIDEFGSGTDPKLGGAIAEAILLELNKR
                     KVYGVITTHYSNLKVLAFKTRGLLNGAMIFDTEHLKPTFQLRVGRPGSSYAFEVASRS
                     GLSPAVIEHARKRTHEDQKELEDLLIELQKDKLVLQEQLASVKEKELKLDKLVHNYER
                     LNAEFELKKKKFKLEQKNWELHSEKALADKIGKTLREIKAVPQPGHGQKKLEEVKQKM
                     ETLDQDLKKMHTEINKLETAQEKPMAIGSYVRLRQGSEIGRIVSIRKDQAQVEMGSLV
                     LNLPLRDLVKTREPLSRTDREQPKKILVEEKKVEGSLDLRGMSKSQAIELLQHYLDRA
                     LLSNVHELRILHGKGNGILRDLVKSQARQYKAIKKIFHPPNEAGGEGISILQLE"
     gene            complement(5678..8356)
                     /locus_tag="PKM27_10715"
     CDS             complement(5678..8356)
                     /locus_tag="PKM27_10715"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNR07775.1"
                     /translation="MRTYVLALLLLSCYSHALTAQNCTTTYADSYSNSTNVDNESRST
                     GAPNGSGATFTDDDSRLTWDMGAGLPTNVQICIVARKGSSGTSAEFNIQYGSNTSSFT
                     TAGSVFEITSTSFASYCVTIPVTFRYIRLVKANNDGTDMQVDAVVATYNCCSAQAGND
                     QSLCLTGSSVTMGATPLAGGSWTVRSSPANPGSVNITDPSSATTSITGFTSPGTYGFI
                     WIGPDCSDTMLVTASNPPSTAVDGSTTACNNSTTAINLFSLITGENAGGTWSRISGSG
                     GTFNAGAGTYVPNGANAGSTQIFRYIQSATAPCINDTSFATVNVIGCCSGRITGLLFN
                     RVTSGGNDLSITNGGSYDRNVIANNYNLEATVSGTLQSVRFIITGPTGSTVIDNDTPY
                     RSPASAAWNPNAGSYSVQVQAWSADNAGGTMCHDTTISFSLFALGSISGRAWLDANNN
                     GQQNTNEYSMMDIEVILTLPGGGTQSAITDEQGEYSFTGLAAGTYSLEFLLPGDKEAT
                     LSNSGSDATDSDIDGSGLISNFTLTAGQNRDYDAGMYAIDCECQGTNLLNNPSFESTG
                     GWTTQSGSFNPRYNNYMMCGSYAAYFQGSSSNSRVYQNVQGIAPGATVTLHGFAGTYA
                     EAANCPPVVRLKFFNAQNGLLQQDEVEISKLILEPDFLQEFFIIGTTAPPNTSYAQVE
                     VAITCHVVKLDGFCLTAEGGGEQQLPIQSLVFNPLEKLNDEIKLSWTTLNELNTDRFE
                     IFRSMDNMQTWVKVSEVPAMGHTTGEHNYVYMDKLDRNLAKISYRINQIDLDGKDQVT
                     PHRTINLKFGQLALEGYPNPTRYLYRLQIQSREEEKLSLVLLDIMGRPVLQENIQVSK
                     GYNMKEITLGSLPAGTYRLRVSGKTSSELITVVKVE"
     gene            8718..9905
                     /gene="lysA"
                     /locus_tag="PKM27_10720"
     CDS             8718..9905
                     /gene="lysA"
                     /locus_tag="PKM27_10720"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNR07776.1"
                     /translation="MISLVSQFGSPLYVYDESVIQRQAHLLKESLAYPDTLFCYAMKA
                     NSNLAILDTVRKLGFGVDCVSRGELELALMAGFDPSVIRFTPSFALTDEIRFAVEKGA
                     QVTLDSLPVLEWYLGQFPDIPAGLRLNPSVMAGAYDKTSTGHAKAKFGIGMDLLAEVL
                     RLVASGPFKINGLHIHSGSDILETDQFMKAVEVLLSAAPRFKSLDYIALGSGFKVPYF
                     EGDKKTDMAQLGKTITDRFTGFCADYGRPLRLIFEPGKFLVSEAGTFLVTCTWVKENG
                     GIRFAGVDSGFNHLIRPMFYGAWHTITNLSNPQGIPEKYDVVGNICETDTFASDRMIP
                     EIRPGDILAFANAGAYCYSMASSYNSRPRPAEVYLQQGEARLIRHRETLQHLLSLQIP
                     LPS"
     gene            complement(9902..10945)
                     /locus_tag="PKM27_10725"
     CDS             complement(9902..10945)
                     /locus_tag="PKM27_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNR07777.1"
                     /translation="MTDNTTYKRGRGAQFNPSNRFNTFHHDEHWICDSKTRFIPTEAK
                     SIVNKVESPDIPMPFSMNPYQGCEHGCVYCYARNTHPYWGYSAGLDFEQKILVKKDAP
                     ALLMKYLSRKNYLPQPILLSGNTDCYQPAEQEFRLTRQILEICLRFRQPLSLITKNSL
                     ILRDLDLLKPMAGLNLVQVILSVTTLDESLRSLLEPRTATSWKRLKTVESLSQQDIPV
                     FVMMAPVIPGLTDHEINELTEQAALHGAWDFFYSLLRLNGDVEPIFKDWLDKTFPERA
                     AKILNRTADCHGGQVQDSRFKVRMKGEGHFAAMVRDQVRIAKTKLGFPKRNAGPLNCS
                     LFKVPKAQLDLFD"
     gene            complement(11150..11629)
                     /locus_tag="PKM27_10730"
     CDS             complement(11150..11629)
                     /locus_tag="PKM27_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNR07778.1"
                     /translation="MKFKEAKALNHVAAFHDLFDAPVLSAPSIPPAERCALRVKLLQE
                     ELKELQESIEQNDLVGIADALGDLQYVLSGAVLEFGLGGVFAEIFDEIQRSNMSKTCR
                     SLEEATRTAAWFENEKKVSTRIVQKGEAYIVYRLPDYKILKSINYSEADLKPFIQPD"
     gene            complement(11619..12101)
                     /locus_tag="PKM27_10735"
     CDS             complement(11619..12101)
                     /locus_tag="PKM27_10735"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR07779.1"
                     /translation="MSNDRLKSIGAHHDQVWQGSPWYGSSVLNGLKGITLPEASGRIA
                     SQHTIAEILYHMTQWKKFVLEKLRGNTAFDIQVDSHADWIIINDLDEASWENIKNQYQ
                     QVTRDLLTEIPNYPDEILDGPVSGRKYTCREMMEGITEHDIYHLGQIMLLKKIMRDEI
                     "
     gene            complement(12164..13268)
                     /gene="prfB"
                     /locus_tag="PKM27_10740"
     CDS             complement(join(12164..13207,13209..13268))
                     /gene="prfB"
                     /locus_tag="PKM27_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156039855.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNR07780.1"
                     /translation="MTIEQLKSLNERLAALRRFLDVGKRIHDIEDKEQLTFNPDFWND
                     PLKAQAVLKEIKINKKWVENFKTIQDQVEELEVLFEYAKEGAVEEAEVELHYQKCLEI
                     MDDAEFRSTLNQPQDALACILEINAGAGGTESCDWSDMLLRMYIMWAEKNDFKVSQLY
                     KVDGDVAGIKSAGLEISGDYAYGLLKGENGVHRLVRVSPFNAQGKRMTSFASVFVHPM
                     IDESIEVQLNPADLEWDTFRSSGAGGQHVNKTESAVRVRHLPSGIVVECQQERSQHQN
                     RDKAIQMLKSRLYEAELERQLEAKEKVENQKMKNEWGSQIRSYVLDDRRVKDHRTGHT
                     EYNTEAVLNGSLNGFLKAFLMWDQNPVAMEEQA"
     gene            complement(13289..14188)
                     /locus_tag="PKM27_10745"
     CDS             complement(13289..14188)
                     /locus_tag="PKM27_10745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNR07781.1"
                     /translation="MLQTMRIGFLLFIGTFLAIQACKPSKRPTTLLGYEYEKHIDKGG
                     RKPKAGEHAYFHAITMVGDSILENTHVYPFVPNMRIEVDPDKEVEAIIDGLKVMGIGD
                     SITLYIPMDSLPFAPAQFQSFKEIKQVLALLDIKDEEGFKQDMAVRQQFLQNLADSLR
                     GRDSVTRLQLTGFITDYLDNKLTDKFQNTEDGVKYLILREGAGERPTTGDMVEVNYVG
                     SLTNQFVFDASFPKGQPYVYRLNTGQVIQGWDSVISQFNEGTEAIVFIPPALAYGKAG
                     SAPNIPPDSELVFYINLYKIRRL"
     gene            complement(14195..15214)
                     /locus_tag="PKM27_10750"
     CDS             complement(14195..15214)
                     /locus_tag="PKM27_10750"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNR07782.1"
                     /translation="MEDLPGIKSLFSLPQNVVILSHRNPDGDAVGSSIALQLYLIRKG
                     HKVHVIFPSEYPVNFTWMPLADEIVIYDLMPGRAKELIQVASVICCLDFNSLDRIDKM
                     GDLVRESPARKIMIDHHIDPEPFCDFVISAPEASATGELIYDMILALDDQKYIYPDLA
                     ECVYTGILTDTGNFSYNISPAIMRKSAHLLELGVDNNKIQELVYNNLPEKQLRLLGYC
                     LYHRMELLPEYQAGLIFLSKEDYSKFSIVRGDTEGIVNYLLMLKSVNIAALITDQNGI
                     IKFSFRSKGDISVQALARENFNGGGHKNASGGYQHTSLKEAIEKFKQVLPKYAQPVSS
                     FNILN"
     gene            15302..15721
                     /locus_tag="PKM27_10755"
     CDS             15302..15721
                     /locus_tag="PKM27_10755"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNR07783.1"
                     /translation="MATNRTFTMIKPDAVASGHIGAILHKINEAGFRIAALKLTRMSR
                     DTAGRFYEVHKNRPFYGELVEFMSSGPIVAAILEKDNAVEDFRTLIGATDPAKAAPGT
                     LRALYARNVGENAVHGSDSDENAAIEAAFHFAGTDLY"
     gene            complement(16005..16511)
                     /locus_tag="PKM27_10760"
     CDS             complement(16005..16511)
                     /locus_tag="PKM27_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNR07784.1"
                     /translation="MKKLFFLLVVGFGLIAACKNENSAVRDEARAAIEGTEAAATTDP
                     NASAAAAIVPAGPTTTIQFMEEEYNFGTINQGEVVSHTFKFKNTGAEPLVITDAKATC
                     GCTVPKKPTEPIAPGETGELVVEFNSAGKSGAQTKQVTVTANTNPPQSFIRIVSDIKV
                     KDAAAGAQ"
     gene            complement(16676..17350)
                     /locus_tag="PKM27_10765"
     CDS             complement(16676..17350)
                     /locus_tag="PKM27_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNR07785.1"
                     /translation="MAYFDPGFSSFLRNLKKHNGREWFLENKTVYEEKVKKPFEALVA
                     ELIYRVHQLEPGMLCTVKDAVFRINRDIRFSKDKSPYKTYVSAVIAHGGRKNLSEPGM
                     YIQLEADRLLIFCGIYMPDKSQLQKIRSAILHQGKEVGQLKSDKKLLALFGSMKGEIS
                     KILPPEFREAARTEPLLAHKQFYFESTYPGMEYLQRKDLAAFIMKHYTAARPWSLFLK
                     EAIQED"
     gene            17451..19541
                     /locus_tag="PKM27_10770"
     CDS             17451..19541
                     /locus_tag="PKM27_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR07786.1"
                     /translation="MSKPPMVNETIPVVYPVTDTSNLLEDYHGSVVNDPYRWLEVDTA
                     TEVESWVKSQNLVTQDYLSKIPFRENIRKRYEELFNYTKLSAPRKIGDYYFYYKNDGL
                     QNQAVIYYQKGRAGREEVFIDPNKLSEKGTVSINLLGASRDDRYLAYSQQAAGSDWQE
                     IRVREIATNTELPDRIKWVKFSGADWDHEGFYYSRYPDPKPGMELSDANRFHSVYYHL
                     LGTGQDRDIKVYENPDEPLMNHSVQVTEDGKYLILYASKGTSGMETRFRAVSDKTKSF
                     QVLFPGFENESYVLTHLDGQFIAVTNVHAPNKKVIRIDPGQPGEANWKTLIPESPHLL
                     NSLSKGGKKLFAEYLENATSKIYQFEYDGSQKTEIELPGLGSAHGFDGKEGDSLLFYQ
                     FTSFTDPGSIYEYRPEEKKSKPFFQPELKFDPAGFEQKQVWYKSKDGTSIPMFLVYKK
                     GLVLDGNNPCYLYAYGGFNISLSPSFSASRLILLENGGVFAMPNLRGGGEFGEAWHKA
                     GMLEKKQNVFDDFIAAAEYLIAEKYTSREKLAIAGGSNGGLLVGACMTQRPDLFAVAF
                     PAVGVLDMLRYHKFTIGHAWAVEYGSSDVKEQFEYLIKYSPLHNLKAGTRYPSTLITT
                     ADHDDRVVPAHSFKFAAALQKAHKGENPTLIRIETDAGHGAGKPTSKIIEEQADIWSF
                     FFYNTNSPVKYMKG"
     gene            complement(19601..20431)
                     /locus_tag="PKM27_10775"
     CDS             complement(19601..20431)
                     /locus_tag="PKM27_10775"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNR07787.1"
                     /translation="MKTKLLVLFLLFLFSPGIAQDLSGLRVLLYTKNGKGYVHDNIPQ
                     AIRCFDSLARKHRFQLKATEDPGVFTAEELTKTDLVVFASTNNEVFETNAQRLAFRQY
                     IEAGGLFLGIHSVLGTERNWTWFKQFLGGTFAWHPRFQPFEVLNIRPDHPSVKAIPVK
                     WVRKDECYFMKELYPGPEVLLVADLQSLKLDSAETVRFELNKGGFARYYPSVWQHRFD
                     GGLAWITALGHDKSDYSNPEFVGHLTQAMGYMKKQRRKLDFSRAYAKTHDEPLLFTNH
                     "
     gene            complement(20428..20976)
                     /locus_tag="PKM27_10780"
     CDS             complement(20428..20976)
                     /locus_tag="PKM27_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNR07788.1"
                     /translation="MLSRKTMLELNRPGLPEMRAGAKIPVTVVLENIRSGLNIGSVFR
                     TADAFNMEKILLAGFSVAPPHKEILKTALDATESVPWARVEDLASALMNLKEKGYHIS
                     AVEQVHGSIPLDQFDFSEKQPLVLVFGNEVSGVSEATLGLCDSAIEIPQSGIKHSLNI
                     AVCAGIVLWESYVQLNSFKNRL"
     gene            21007..22446
                     /locus_tag="PKM27_10785"
     CDS             21007..22446
                     /locus_tag="PKM27_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07789.1"
                     /translation="MKFPTGATGKYLPYLALYFMLLLAHGYQYGSGDQSDFMPYALHL
                     RNPDLYPGDFYISCLKEHFNERWLIARILSLIPPAGWPVLFMIIHFLASVLLFNGLLK
                     FSAHFLSRAWMQWLLLSVLLIVLYPVHLGGNELYYNMVCPSLLSKAAGIWCLWFCFRQ
                     DDLKAALCAVLATWLHPVAGFQVFILGTAFLPAVKWGRYCIITLTAVAPYLFILLKNL
                     TGPSQILPEVMALRNAHHFYPAYFGLKNAIILLPIFFAGTALAYRKDRRIFRLGIWVL
                     LGCGVYLLTLSAYPDLAIKSQWFKSTIWLEFFSLLMIGTWLSDLTLLRDVLKRLGSPF
                     LMLVYLIVLVVALYRARLANWYFPWSAPNESIRTALEAKSVSQPGDLFIVPAGFTSFK
                     FFAERPCWLDWKAIPHRSDCLEEWAGRVEKAYGLTTGYQGNLQEIDRISNARLASLDQ
                     ASKEMLKAAGVKYVLFRPEGKTDYRMIKL"
     gene            complement(22441..23688)
                     /locus_tag="PKM27_10790"
     CDS             complement(22441..23688)
                     /locus_tag="PKM27_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR07790.1"
                     /translation="MNKTGLYLSRQETRHPLLNQPPDPDTAFIVVIPVFNETDPEPCL
                     QSIEEAAGFASQKVEILIVVNDTDKSPETAVRQNRISLESLPKRSGACPLFVLDRTGL
                     PHKKGGVGPARKTGMDEACFRFFKAGRPGGVICSLDADTRVEANYFLVLRDAFRNTSK
                     ACLLIHYEHDTDNLPSKRHLEAITDYELHLRYYVHALRYAGFPYAFQTLGSAFAVDAA
                     AYASEGGMPPRQAGEDFYFIHKFSVKNQVASIGGTTVHPSSRLSDRVPFGTGKALLDR
                     MDGPPVEMMTYALRNFEVIRALLTQLEALYFQKSFRSLNLEKGLIEFLDTIGFEARVS
                     EMKKHSPSFKTFRNRFFQGMNAFMLMKCLHFLRDRDYANEPLGTACRQLLDRMGLEGM
                     KHGNHMDMLAYLRLKDKVLYMPS"
     gene            complement(23685..24374)
                     /locus_tag="PKM27_10795"
     CDS             complement(23685..24374)
                     /locus_tag="PKM27_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR07791.1"
                     /translation="MNKSKILLAEDDETLAFVTADNLNAKGYQVILVNNGVEAIKAFE
                     AEKPDLCILDVMMPLKDGFEAAKEIRAKSKEVPILFLTAKSMKEDRIEGLSLGADDYI
                     TKPFSIEELVLRIEIFLRRRFVDPNRTSGVITIGRYTFDPENYTIEGPEGKQGMTQRE
                     AELLSYFFKHKNQLSKRSEILVAVWGKDDYFLGRSMDVFVSRLRKFFEKDPSIKIENV
                     HGVGYRFTAPE"
     gene            complement(24371..25639)
                     /locus_tag="PKM27_10800"
     CDS             complement(24371..25639)
                     /locus_tag="PKM27_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR07792.1"
                     /translation="MRSSVIARLIILGSIAILSTLFIQFYIIRQQQNVEERAFRDKAI
                     TALSKVAKRLSKNDDLESFTSLIKHPQSNYFLVNINDHINVNTLDYYLKVEFQNVGLK
                     ENYEFGIYDCDRDSMVYHNAVSYEANQKNIRPGTKLPTYKDYLYYFGVRFPDRQANPF
                     YSYTWVSLGLLMLILLFFAYSLWIILKQRTLAEMQKDFINNLTHEFKTPLAAVNISSD
                     VLLQNEVIQENPRLRQYAQIIKNQNQLLNTQVERVLQVARMEKTNFQILPEPVNIHEI
                     ITDLVSGTEPRIKAKNGEIHVQLNAMAPVISADRIHFIHIIQNVLDNAIKYSEDHQLK
                     IELETYDEADKMVIRCADHGSGIPKEYLKKIFSRFYRVPTGNVHNVKGFGLGLHYVYQ
                     ICKAHGWKIRIESQVGVGTSLYIEIPKVIS"
     gene            25731..26312
                     /locus_tag="PKM27_10805"
     CDS             25731..26312
                     /locus_tag="PKM27_10805"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNR07793.1"
                     /translation="MDDATTLVATEYFPRISTVRQMMLAQKVWLAYGETYQKNSLRNR
                     CYLASGQGLQRLSVPLRKGKHQSQKIYKVKIAYEEDWPLQHLKAMETNYRSAPFFLHF
                     VDPVRLILESRPEYLVDLNWKILSLLFNVFRWHPDIACHMEFSSPAKSTISPTVKLRP
                     YNQVFAGRFGFLADLSILDLLFCMGPEPKYILP"
     gene            26359..28098
                     /locus_tag="PKM27_10810"
     CDS             26359..28098
                     /locus_tag="PKM27_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNR07794.1"
                     /translation="MSVFQFDPKIEVYQSLIDEAVKNLHELTQEIQNKELMETVSSLR
                     ARIRDPYMFVVVGEVKAGKSSFINALLSAGREICKVAPQPMTDTIQQIVYGPEEQEVH
                     INPYFKRHFIPAEILKEIAIVDTPGTNTIVAHHQEITERFIPSSDLIVFVFEAKNPYR
                     QSAWDLFDFIKDEWRKKIIFVLQQKDLMSPEDLAVNEKGLAEYAFKKGLPSAKIFSVS
                     AKDELEGRTDQSGFTLLRNYINANILQGKAGFIKLYNNVSTAQNINSRIYKGILDRKE
                     QFLYDLNFRNDIRTTLDEQEAKSKQQVAVLAENLLAAYDRATLKTEKELEAGLGMFNM
                     VKRSLISVFSKSQSVKEWLTGLLSGLEKDLQIELQNKLTGGIGDIADNIQQMGKIIDL
                     KIRNSRTVLKDSHELFAEIAERRAGVFRELQETFTSFLNRSENFSSADLFPGQESLSP
                     QIAGGTGMGIVGIVLAAIAKGTIFDVTGGILSGLGFLFAGALAFFQKRKFMDSFRKEI
                     NKGRNDLDSEVKARLNQYIVNIRQKIERSFSNFDGLLQYEEKQLARLDGFHNKIKDDL
                     SGLETELQGYQLA"
     gene            complement(28103..28534)
                     /locus_tag="PKM27_10815"
     CDS             complement(28103..28534)
                     /locus_tag="PKM27_10815"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR07795.1"
                     /translation="MKIHPVAFGTPEYDELIRLRYRVLKEPLGLDFSEEDLSKEFEDT
                     HVALYDDAYQLLACLVLSPETGGQVRMRQVAVLPEKQNSGFGRELVGYSEDWSRRAGF
                     KEMVLHAREQAIPFYEKLGYKKAGKKFTEVGLDHWQMKKSL"
     gene            28572..>29456
                     /locus_tag="PKM27_10820"
     CDS             28572..>29456
                     /locus_tag="PKM27_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR07796.1"
                     /translation="MKPIFAALLAMLYFHPLSAQRIAKVKKEAAAPVVTDLRPIVDQM
                     ANEIENEIIEARHWLHQHAELSNREFKTAEYIAKYLASVGIEHQTGVGKTGVVGLIRG
                     AKPGPTILLRADIDGLPVTERVDLPWKSTNRDLYLGMQVGVMHACGHDTHAAMLMGAA
                     KILYGIRDQLKGNIKLVFQPAEEGPPPGEEGGASLMIKEGLMENPAVDVAFGLHINSS
                     TEVGHINYKPGGTMAASDVLRIKIKGKQTHGSTPWDGIDPVVVGAQIVMALQTVVSRQ
                     TELTKEAAVVTVATFHAGV"
BASE COUNT         7091 a         7398 c         7448 g         7519 t
ORIGIN      
        1 tgagatcatc gacaaatcca tgtaccccgg ggcgaccatc gaagacaaac tgggcaaaat
       61 gatcaattcc gattttctga atggcctgga agtactctat gccatcgata agatttcatc
      121 ccggatcgaa accctgggca tcgggaaaaa acaaatcaac tataaaatgc gggatgccaa
      181 ttattcaagg caaagatatt ggggtgagcc gttcccgatc atttatgacc cggagggcat
      241 accgcatacc ctgcctgtag aggaattacc cttgaccttg ccacccctgg atgattttcg
      301 tccgggttcc aacggcaagg cccctttatc caaactggaa tcctgggtca agctgcccaa
      361 cggatatacc cgggaaaccg acaccatgcc cggatttgcc ggctcatcct ggtactttct
      421 ccgctatatg gatccgcaca acgaccttga atttgcctcg gcctcttcga tccggtactg
      481 gcaggatgtg gatctttacg taggaggcac cgaacacgcc gtagggcatt tgctttattc
      541 aaggttctgg cataaatttt tattcgatct gggttatacg gtcaccaaag aaccctataa
      601 aaaactgctc aaccagggca agatccaggg catttcagaa tccgcctttc tcctgaagga
      661 aaaacagggg ggccggcacg tatttgtttc ccgtacgctg atgggtccgg acgatcataa
      721 ggactgcatt gcccttccgg tgcctatcga atttgtaaag gattacggaa gcatgaacgc
      781 atacctggat gcgggaggaa tccggcaatt catccggttt atgccggatt acggggaggc
      841 cctgttcaaa acggaacagg gaatttatac ttccgaaaat ttccccccgg aaggccgttt
      901 ttacacccag gcagaactgg ccaaaatgtc caaatccctg cacaatgtca tcaatcccga
      961 tgaggtggtg gcggaatacg gggcggattg tttccgcttg tttgaaatgt ttctgggtcc
     1021 cctggaacaa tccaaaccct gggatacgcg gagcatagac ggagtaggaa aatttgtccg
     1081 caggatatgg tccatgtttt ttaacgaagg gaaatttgag gtgtccgggg aaccggccac
     1141 ggcggaggaa cttaaaatac tgcatagatg catcaaacgg gtgaccgagg atattgagcg
     1201 gttctcattc aatacctgcg tttcccattt tatgattgcc tctaacgaac ttaaagcggt
     1261 ccattgcaac aagcgggaga tcctggaccc cttcatccgc ctgatggccc cttttgcccc
     1321 ttttcttacg gaagaactct gggaacggct gggacatggc gaatccattc acctcaccgg
     1381 ctatccggag gctgacccgg cacacctgtt tgaaagtgtg gtgaattatc cgatctgcat
     1441 caatggcaaa aagagaggaa atgcagatta ccctgcggac gcagaccctg cttccctcga
     1501 aagcatgacc ctcggactcg attttgtgca gaaatggctg gaagggaaaa cgcctaaaaa
     1561 agtcattgtg gtcaccggaa agatgatcaa tatcgtgctc tgacctttag tggatacgga
     1621 cggtctgtat taaatcaatg gttagaatgc caccctccac cctcttgttc gcaagccaaa
     1681 acagaaagaa agcggaagaa atccgagcca ttctgggcga tgcttttcag atccgcacct
     1741 tggccgaaat gggctatgaa caagacctgg atgaaccctt tgatactgtt gaggccaatg
     1801 caaagcataa ggcacggcag ggttttgaac tctttaaact gccctgcttc gcagaggata
     1861 ccggcctggt ggtggaagcg ctgaatggcc tgccgggagt gcagagcgcg cggtatgccg
     1921 ggaaaaacaa agacccggaa gccaatatgg agaaagtcct ttccgaaatg aaggggatca
     1981 ccaaccgccg ggcttatttt atcgccttca ttgcctacta cgatggcctg gagttttttc
     2041 tttttgtagg caggatccat ggtcttatcc tgcatgaaaa gcatgggaac ggtggttttg
     2101 gttatgatcc tattttccag ccggacggtt ttgaacggag ttttgcagag atggattctt
     2161 ccattaaaaa caggatcagt caccgggccg gtgcgctcca cggttttacg tcctggttaa
     2221 aaggaaaggg ttagttagtt agtaaggtgg caagttagta agttggcaag ttagtaagtg
     2281 ggatggggtt aaaacaacaa atccatgcta aacaggactc cataaatcac gctggccagg
     2341 ccattggtag taaagaaagc gagattgatt ttgctgagat ctcccggctt cacaaggttt
     2401 tgctggtata tcaacatgga tacaaaaacc agggcgctca gccaaagcca tacgccggaa
     2461 ctttcatacc ggttatgaat cagccaactg gcaaaaaaaa ggatcatggc gctgatgaaa
     2521 tgcaggagcc ggctgatctt cagggctttg gattttccga accgggcagg tattgaatgc
     2581 aggcggtgga ccttgtcaaa ctcctcatcc tgcaaggcgt aaataatatc aaaaccggag
     2641 acccagtaaa acaccacccc ggaataaagc agggggatca gggaaaagat gccggtgacg
     2701 gcaagataac tgccaatggg cgccagcgcg aggcctcctc ccaggatgag atggcaaatg
     2761 gcggtccatc gtttggtata actgtatcca agaatggtca ggagggccag gggcgacaaa
     2821 taaaaacaga tggaattgat cgaccaggtc gtgaccacaa aagccaggct gctgatcagc
     2881 gtcatccata aagcttcatg ggaagtcacc cgtccggccg ggatttcacg tcccgcagtc
     2941 cgcggattaa gccggtcgta ccgttcatcc gcccagcggt tgaaggccat ggccgcgctc
     3001 cgcgcaaaca ccatgcaaag gagaattttg agcccgagga gccaatccaa tgcatatccg
     3061 gccttaaagg ccaggaccgt tccgactgct gcgaaaggca aagcaaaaac ggtgtgggaa
     3121 aatttaatga gtgacaggta tgatttcatt cactgtttta ttccagttga aggatggaga
     3181 tgccctcgcc gcccgcttca ttgggcgggt gaaagatttt ttttatggct ttatattggc
     3241 gtgcctggga tttgaccagg tccctcagaa tgccgttgcc tttaccgtgc aggatcctca
     3301 actcatggac gttacttaac agggcccggt cgagataatg ctgcagcagc tctatagcct
     3361 gggatttgct catgccccgg agatcgaggg agccttccac ttttttttct tccaccagga
     3421 tctttttggg ctgttcccgg tctgtgcggc ttaagggctc cctggttttt accaggtccc
     3481 tcagggggag gttcaggacc agagatccca tttccacctg ggcctggtct ttgcggatag
     3541 agacgatccg gccgatttcc gacccttgcc ggaggcgtac gtaagaccct atggccatcg
     3601 gtttttcctg tgccgtctcc agtttattga tctccgtgtg cattttttta aggtcctggt
     3661 cgagggtttc cattttttgt ttaacctctt ccagtttttt ctgaccatgt ccgggctgag
     3721 gaaccgcctt tatttcgcgg agggttttcc cgattttatc agccagggct ttctccgagt
     3781 gcaattccca gtttttctgc tcgagtttaa attttttctt ttttaattcg aattccgcat
     3841 tgagcctctc gtaattgtgg accagcttat ccagtttcaa ttctttctct ttcacactgg
     3901 ccaactgttc ctgcaggacc agtttatcct tctggagttc aatcagcagg tcctccagtt
     3961 ccttttggtc ttcgtgggtc ctttttcggg catgttcaat caccgcaggg gacaggccgc
     4021 tgcgggatgc gacctcaaag gcataggaac tccccggcct gcccaccctg agctgaaacg
     4081 tgggcttcag atgttccgta tcgaagatca tggccccgtt gagcaggccc ctggttttaa
     4141 aagccaggac tttaaggttg gaataatggg tcgtgatgac cccgtacact tttcttttgt
     4201 ttaattccag gagaatggct tccgcgatcg cgcctccgag tttgggatcc gtcccggacc
     4261 cgaactcatc gatcagaacc aggctccggt catccgccac ctccaggaat tgtttcattt
     4321 tcagcaggcg ggaactgtaa gtggaaaggt cgtcttccag ggactgttgg tccccgatgt
     4381 ccccgagaaa atggtggaaa acacccattt cactggcttc atgggccggg ataagcatcc
     4441 cggactgggc cataagttgg atcaaaccga ccgctttcat ggccacggat ttgccgccgg
     4501 cattggggcc gctgagaatg agcacccggt ggtctcctgt caggatgaga tcaaagggga
     4561 cggtttccct gcccaggcct ttatttttta aaaaaaggag cgggtgcctg cctgccttga
     4621 ttttgagttg tggacggtcg gtcacttcgg gccggtatgc ctggcagttt actgcaaata
     4681 ttgctttgga tcggataaaa tcgaaatgca agagcatctc ccatcccatg cgtaacatat
     4741 ctgcatgctg ccggcaggat ttgctcaggg cctggaggat ccggtagatc tccttttgat
     4801 aatccatttc caggtcaaac agctcattgt ttttttcgat gatgcgttcc ggctcaataa
     4861 aggtggtcct gcccgtggtg gattcatcgt ggatgatgcc tttaatcctg cgcttgtatt
     4921 ctgattgcac cgccaccacc cttctgttgt tgcggacgct ttctcccgat tcactcaacc
     4981 agccctgttt gcggaattca ttgagcaggg accggaacac ctggtcgatt tcttcttctt
     5041 ttgaccggat tgcccggctg atgatcttca gttcaggggt cgcatccggc ctgatctggc
     5101 cggagtcgtc aaagatcgtc ctcatttccc ggtaaaggtc cggggtatag ttcaaccgtt
     5161 caaattgccg gattgagagg ggcatggcct tgacctggtc cggattcctg aaaaataaag
     5221 cgatttcttc caggttttta accacctgga agagatggac cagggcctcc agatcgaaca
     5281 cataatcctg gatgtccgtc ctcttgaggc tttctgaaat acttggacag gcttgcaccg
     5341 ggaggaggct attcctttca taagctgatt tgaattcgag ggtttcatcc aaggcaaggt
     5401 caatggcctg cttggaggtc atgaccgcca tgggaaagta cctgctccgg gtgtcttcaa
     5461 tcaggcagag cgccgccacc gaatgattaa gtttgtcgaa ttccaggtgt tgaaataagg
     5521 cttcgggccg gaccagtatc atgagggtac aaaaataggg aaaaaaaaag ccggcaatat
     5581 ccaggatact gccagctgac cgctacctag tgtaaattta gtagggtatt ttttgataca
     5641 tgaggacctg aacccgaata ttgccaggtc cggagcgtta ttccactttt actacggtga
     5701 tcaattcact ggacgtcttt ccggaaaccc ggagtctgta ggtacccgcc ggaagcgatc
     5761 caagcgtgat ctccttcatg ttgtatcctt tggaaacctg gatattttcc tgcagaaccg
     5821 gccggcccat aatgtccagg aggaccaggc tgagtttttc ctcttcccgg ctctggatct
     5881 gcaggcgata caggtaacgg gtgggattgg gatatccctc caaagccagt tgcccaaatt
     5941 ttaaattgat cgtccggtgc ggggtgactt ggtcctttcc gtccaggtca atttggttta
     6001 tccggtagga tattttggcc aggttcctgt ccagtttatc catgtaaaca taattgtgct
     6061 ctcctgtggt atgccccatg gccggaactt cgctgacctt tacccaggtc tgcatattgt
     6121 ccatgctcct gaatatctca aagcggtccg tgttcagttc gttcagggtg gtccagctta
     6181 atttgatttc atcgttcaat ttctccagag ggttaaatac cagggattgg ataggcaact
     6241 gctgttctcc gccaccctcc gccgtgagac agaatccatc caacttgacg acatggcagg
     6301 tgatggccac ttctacctgg gcatagctgg tattgggggg cgcagtagtc ccgataatga
     6361 aaaactcctg gaggaaatcc ggttccagga tcaatttgga gatctccacc tcatcctgct
     6421 gcaacaggcc gttttgggcg ttgaaaaact tcaatctgac taccggggga caatttgccg
     6481 cttctgcgta tgtgcctgcg aatccatgca gggtgacggt tgcgcccgga gcaatgccct
     6541 gtacattttg gtaaaccctt gaattggaag agctgccctg gaaataggcg gcataacttc
     6601 cacacatcat ataattgttg tacctgggat taaaactccc gctttgggtg gtccagcccc
     6661 cggtgctttc aaaactgggg ttattcagca gattggttcc ctgacactcg caatcaatgg
     6721 catacatccc ggcatcgtaa tcccggtttt ggccggcggt cagagtgaaa ttgctgatca
     6781 aaccactgcc gtcaatatcg ctgtctgttg catcgcttcc ggaatttgaa agcgtagcct
     6841 ctttatctcc gggcaataaa aactccaggg aataggttcc ggccgccaat ccggtaaagg
     6901 aatattctcc ttgctcatcc gtgattgcag actgggtacc tccgccgggc agggtgagaa
     6961 tgacttcaat gtccatcatg gaatattcat tggtattttg ctgaccgttg ttgttggcgt
     7021 ccagccaggc acggccgctg atgcttccta gggcaaaaag actgaaggaa atggtggtat
     7081 cgtggcacat ggtccctccg gcgttatcgg ctgaccaggc ctggacctgg acagaatagg
     7141 agcctgcgtt tgggttccag gcggctgaag ccggtgaacg gtagggcgtg tcgttatcaa
     7201 taaccgtgga accggtaggg ccggtgatga tgaaccggac actctggaga gtcccggaaa
     7261 ccgtggcttc caaattataa ttattcgcta ttacattgcg gtcgtagctg cccccgttgg
     7321 taatgctgag gtcattgccc ccgctcgtga cccggttgaa taagagcccg gtgattctgc
     7381 cggaacaaca accaataaca ttcacagtcg caaaactcgt atcgttgatg cagggagcag
     7441 tggcggactg aatatatctg aatatctggg tggagccggc gttggcgcca ttgggcacat
     7501 aggtgccggc cccggcgttg aatgtacccc ctgagccgct tatcctgctc caggttcctc
     7561 ctgcattttc accggtgatg aggctaaaaa gattgatggc cgtcgtgctg ttgttgcagg
     7621 cggtggtgct gccgtccacg gcggtactgg gtgggttgct ggcggtgacc aacatggtat
     7681 ccgagcagtc cggaccgatc cagataaagc cataggtacc tggtgaagta aaccccgtaa
     7741 tcgaggtcgt ggctgatgaa ggatcagtga tgttgacact gccggggttg gccggacttg
     7801 acctgacagt ccaggaccca ccagccaggg gggtagctcc catggtgacg ctggagcccg
     7861 taaggcaaag cgactgatca tttccggctt gggctgaaca gcagttgtaa gtagccacaa
     7921 ccgcatccac ctgcatgtcc gttccatcat tatttgcttt taccaaccgg atgtatctga
     7981 aagtgaccgg tatggttacg caataactgg caaaactggt gctggtgatc tcaaagaccg
     8041 atcccgcagt ggtaaaactg gaagtgttcg atccatactg aatgttgaat tctgcggacg
     8101 ttcccgagga accttttctt gcgacaatac agatctgaac attggttgga agaccggctc
     8161 ccatatccca ggtcaaacgt gaatcgtcgt cagtaaatgt agcaccagag ccgttggggg
     8221 cgccggtgga ccgactttcg ttgtctacat tagtggagtt ggaataactg tccgcataag
     8281 tggttgtaca attttgggca gttaatgcat ggctgtagca gctcagcagc agcaatgcaa
     8341 gtacataagt acgcatttta tttagtgtta aagggtgtta atagacttga ggtcgattaa
     8401 cggtgcaaaa ttataacaat tattaccgaa ttttatataa tgcaattgtt aaatattgag
     8461 gtctaggcaa ttttttattg gttattaatt tgagcctttt taatcattta ttaatctatt
     8521 tcatacatat tacatttatt ttaatatttt atatattctt aaatagtaaa ttatataccc
     8581 ttctaaataa atccggagga taaagaggta tgccgtatca aactcttgtt taaagcggtc
     8641 ttcgacaata ccaaattttg tttcgcatcc tggctttctt tattatactt gcagttcaat
     8701 gtttaagttt ttcatccatg attagcctgg tatcccaatt tggcagccct ttatatgtct
     8761 atgatgaatc tgtcattcaa aggcaggccc acctattgaa ggaatccctg gcctatccgg
     8821 ataccctttt ttgttacgcc atgaaggcca acagcaacct ggccatcctg gatacggttc
     8881 gaaaacttgg ttttggggtg gattgtgttt ccagggggga actcgaactc gccctgatgg
     8941 ccggattcga tccttccgtc atccgcttta ccccgagttt tgctttgact gacgagatcc
     9001 gattcgcggt ggaaaagggg gcacaggtca cgctggacag ccttcccgtt cttgaatggt
     9061 atctcggtca gtttccggat attccggccg gcttaagatt gaatccttcg gtcatggccg
     9121 gcgcctatga caagacctct actgggcatg ccaaggcgaa attcgggata gggatggatc
     9181 tcctcgccga ggtcctgaga ctggtagcct ccggtccatt caaaatcaac ggattgcata
     9241 tccacagcgg tagcgatatc ctggaaacgg accagtttat gaaagcggtc gaagtcctcc
     9301 tttcggcagc cccccgattc aaatccctcg attatatcgc cttgggcagc ggttttaagg
     9361 tgccttattt tgaaggggat aaaaaaacag acatggccca gctgggtaaa acgatcaccg
     9421 accgttttac cggcttttgt gcggattacg gccggcccct ccggctcatt tttgagcccg
     9481 gaaaattcct ggtcagtgaa gccggcacct tcctggtgac ctgtacctgg gttaaagaaa
     9541 acgggggcat ccgttttgcg ggggtggaca gcggattcaa ccacctcatc cggcccatgt
     9601 tttatggcgc ctggcatacc atcacaaacc tcagcaaccc ccagggcatc ccggaaaaat
     9661 atgatgtcgt agggaacata tgcgaaaccg atactttcgc ctctgaccgg atgatcccgg
     9721 agatccgtcc cggcgacatc ctcgcttttg ccaatgcagg cgcttattgt tattcgatgg
     9781 cttcctctta caattcacgc ccaagaccgg cggaagtcta tctgcagcag ggggaagccc
     9841 ggctcatccg gcatcgcgaa accctgcaac acctgctctc cctccagatc ccactgcctt
     9901 cctaatcaaa cagatcaagc tgggctttgg gcaccttaaa aaggctgcaa ttcaacggac
     9961 cggcgttccg cttggggaaa cccagctttg ttttggctat cctcacctga tcgcggacca
    10021 tcgctgcaaa atggccttcg cctttcatac ggaccttgaa tcttgaatcc tgcacctggc
    10081 cgccgtgaca gtccgcagtc cggttcagga ttttggcggc cctttccgga aaggtcttat
    10141 ccagccagtc tttaaaaatt ggttccacat ccccgttgag ccgcagcagg gaataaaaaa
    10201 agtcccaggc gccgtgaagc gctgcttgtt ccgtgagctc atttatttca tgatccgtga
    10261 gtcctggaat aacgggcgcc atcatcacaa aaacggggat atcttgctga ctcagggatt
    10321 ccacggtttt taaacgtttc catgaagtgg ccgtcctcgg ttcgagaagg ctcctcaggg
    10381 attcatccag ggtcgtcacg gaaaggatta cctgcaccag gttcaagccg gccatgggtt
    10441 tgagaaggtc cagatccctc agaatcaggc tgttcttggt aatgagactc aggggctggc
    10501 gaaagcgaag acagatttcc aggatttggc gggtcagcct gaattcctgc tcggccggct
    10561 ggtagcagtc tgtgtttcct gaaagcaaaa tgggctgagg caggtagttt tttcggctga
    10621 ggtatttcat cagcaaggcg ggagcatcct ttttgaccag gatcttttgc tcaaaatcca
    10681 atccggcgct gtaaccccaa tagggatggg tgtttcgggc gtaacaataa acacaaccat
    10741 gctcacaccc ctggtaggga ttcatggaaa aaggcatggg tatatccggg ctttccactt
    10801 tgttgacaat ggattttgcc tcggtcggga taaacctggt cttggaatca cagatccagt
    10861 gttcgtcgtg gtggaaggta ttgaagcggt tggaggggtt aaactgcgca ccccggcccc
    10921 ttttgtatgt cgtattatcc gtcatgaata gtcgaatata cagacataat tacagagaaa
    10981 caaaaaaaaa gtcccgactt ttcgggacca aagcgtggat atgttctagg ccttgatact
    11041 tgctaagttg gtgacaagca agtggatcaa acctcaaaaa tgtaaattta taaataaaat
    11101 aggccgtgat gggttaaata aggcccactt taataaaaaa ttaaatttat caatccggct
    11161 ggataaaagg tttcagatcc gcctcggagt aattgatgga tttcaggatc ttatagtcgg
    11221 gcaggcgata tacgatatag gcctccccct tctgtacgat ccgggtggaa actttttttt
    11281 cattttcaaa ccaggctgcg gtccgggtag cttcttccag gctgcggcag gtcttgctca
    11341 tattggatcg ctggatctca tcaaatattt cggcaaacac cccgcccagc ccaaattcaa
    11401 ggaccgctcc ggataaaacg tattgcaggt ccccgagggc atcggcaatg ccgaccaggt
    11461 cattctgttc gatcgattcc tgcaattctt ttaattcctc ctgcaacaac ttgacccgga
    11521 gggcacatcg ttccgcaggg ggtatggaag gagcagagag gaccggggca tcaaacaaat
    11581 cgtgaaaggc ggccacatga tttagcgcct tcgcttcttt aaatttcatc ccgcatgatt
    11641 tttttcagga gcatgatctg tcccagatgg tagatgtcgt gttccgtgat cccttccatc
    11701 atttcccggc aggtatattt cctgccgctt accggaccat ccaggatttc atccggataa
    11761 ttcggaatct cggtgagcaa gtcccgggtc acctgctgat attgattttt gatattttcc
    11821 caggatgctt cgtccaggtc attaatgata atccagtctg catggctgtc cacctggatg
    11881 tcaaaagcgg tgttccctct cagtttttcg aggacaaatt ttttccactg ggtcatgtgg
    11941 taaaggattt cggcaatggt atgctggctg gcaatcctgc ccgaggcctc cggaagagta
    12001 attcctttga gcccgttcag gacggaggac ccataccagg gggaaccctg ccagacctgg
    12061 tcgtggtggg ctccaatgga ttttaaacga tcgttgctca tcttgcggtc ataaatatcc
    12121 agaaaaagga ggatctaaat ttttaaatct gtaaagcata aggtcaggcc tgttcctcca
    12181 tggctaccgg gttctgatcc cacatcagga acgctttcag gaagccattg agcgatccgt
    12241 tgagcaccgc ttcggtattg tattccgtat gcccggtccg gtggtccttg accctccggt
    12301 cgtccagcac ataagaacgg atttgactgc cccattcgtt tttcattttc tggttttcaa
    12361 ccttttcctt agcctccagt tgcctttcca gttccgcttc gtaaagcctg gattttaaca
    12421 tctgaatagc tttatcgcgg ttttgatgct gggaacgctc ctgctggcat tctaccacaa
    12481 tcccggaagg caggtggcgc acccgcacgg cggattccgt cttattgaca tgctggccgc
    12541 ccgccccact ggaccggaag gtgtcccatt ccaggtcggc cggattgagt tgcacctcta
    12601 tgctctcgtc gatcatcggg tgcacgaaga cggaagcgaa ggacgtcatg cgtttaccct
    12661 gggcattgaa tgggctgacc cggaccagcc ggtgcacgcc attttcccct ttaagcagcc
    12721 catacgcata atcgccggaa atctcgagac ctgcactttt aatgccggcc acatctccgt
    12781 ccaccttgta cagctgggaa accttaaaat cgtttttctc cgcccacatt atgtacatcc
    12841 ggagcagcat gtcggaccag tcgcaacttt ccgtgccgcc ggcgccggca ttgatttcga
    12901 gaatacaagc cagggcgtcc tggggttggt tgagcgtaga cctgaactcg gcatcatcca
    12961 tgatttccag gcacttctgg taatgaagct ccacctcggc ttcttctacc gcaccttctt
    13021 tggcgtattc aaacaaaact tcaagctctt cgacctggtc ctggatcgtt ttgaagtttt
    13081 caacccattt tttgttgatc ttaatttctt tcagcaccgc ctgtgctttc aggggatcgt
    13141 tccagaaatc ggggttgaag gtcagttgtt ctttgtcttc gatgtcgtga atacgtttcc
    13201 cgacgtcaaa gaaacctcct tagcgcagcc agtcgctcat ttagtgattt taattgttct
    13261 atggtcatga ttgaaataat ttttacagtt acagtcttct gatcttatac aaatttatgt
    13321 agaataccag ttccgaatcc ggaggtatgt ttggagcgga ccctgctttc ccgtaggcca
    13381 gcgccggggg aatgaaaacg atggcttccg tcccttcgtt gaactgggag atcaccgaat
    13441 cccaaccctg gatgacctgc ccggtattca gccggtatac atagggctgg cctttgggga
    13501 aggaggcgtc aaaaacaaac tggttggtta gtgagccgac atagttcacc tccaccatat
    13561 cacccgtggt gggccgctcg ccggcccctt cccggaggat caaatatttt accccgtctt
    13621 ccgtgttttg aaatttatcc gtcaatttgt tatccaggta gtctgtgatg aagcccgtca
    13681 actgaagcct ggtgaccgaa tcacgccccc tcagggagtc ggccaggttc tgcaaaaact
    13741 gttgccggac ggccatatcc tgcttgaatc cctcttcatc tttgatatcc aggagggcaa
    13801 gcacctgttt gatttcttta aaactctgga actgggccgg ggcgaagggc agggaatcca
    13861 tggggatata aagggtgatg ctgtctccga tgcccatgac cttcagtccg tcaatgatcg
    13921 cttccacctc cttgtccgga tccacctcga tgcgcatatt ggggacaaag ggatagacgt
    13981 gcgtgttttc gagaatggaa tccccgacca tggtgattgc atggaaataa gcatgctcgc
    14041 cggctttggg tttccttccg cctttgtcaa tatgcttttc atactcataa cccaataaag
    14101 tagtgggccg tttggagggc ttgcaggcct ggatggccag gaaagtaccg ataaacagca
    14161 aaaagccgat tctcattgtt tgcaacatgt atgattaatt taaaatatta aatgaggaga
    14221 ccggctgggc atactttgga aggacctgct tgaatttctc aatggcttcc ttcaggctgg
    14281 tatgctgata gccgccgctt gcatttttat ggcctccccc gttaaaattt tcgcgggcaa
    14341 gggcttgtac cgaaatatct cccttggacc ggaaagaaaa cttgataatg ccgttctgat
    14401 cggtgatcag ggctgcaata ttgacggatt tgagcatgag caggtaattg acaatacctt
    14461 ccgtatcgcc ccgtacgatg gagaatttgc tgtaatcctc cttactgaga aagatcagcc
    14521 cggcctggta ttccgggagc aactccatcc ggtggtacag gcaatacccc aataagcgca
    14581 gttgtttttc aggaaggtta ttgtagacca gttcctggat tttattgttg tccaccccca
    14641 attccagcaa atgggcggat tttctcatga tggccggcga aatgttgtat gaaaaattgc
    14701 ccgtgtccgt gaggataccg gtatacacgc actcggccag gtcgggatag atgtattttt
    14761 gatcgtccag ggccaggatc atgtcgtaga tcagctctcc ggtggcgctg gcctcgggtg
    14821 cagaaatcac aaaatcgcag aacggctccg ggtcgatgtg gtgatcaatc atgatcttcc
    14881 gggcggggct ttccctcacc agatcgccca ttttatcgat ccggtccagg gaattaaaat
    14941 caaggcaaca gatcacggaa gctacctgga tcaattcttt ggcccgtccg ggcatcagat
    15001 cgtagatgac gatctcatcg gccagcggca tccaggtgaa gtttacaggg tattcactgg
    15061 ggaagatcac gtgtacttta tgtcctttgc ggatgagata aagctgcaag gcaatgcttg
    15121 agccgacggc atcgccgtcg gggttccggt gggacaagat caccacgttt tgaggcagag
    15181 aaaacagaga ctttatcccg ggcaggtctt ccataaaaac ggcgcaaaga tcgggaaatt
    15241 tgacgaattg agcacaatat tagtgacagc ggattacctt tgcggcgcaa aaaaacatct
    15301 gatggcaacc aatcgcactt ttaccatgat taaaccggat gccgtggcat ccgggcatat
    15361 cggcgcaatc ctccataaaa tcaatgaagc cgggttccgg atcgccgcct taaaacttac
    15421 ccggatgtcc cgggatactg cgggcaggtt ctatgaagtc cacaaaaaca ggccgtttta
    15481 tggtgaattg gtcgagttca tgagttcggg cccgatcgtg gctgccatac tggaaaaaga
    15541 caatgccgtg gaggattttc ggaccctcat cggagctacc gatcccgcaa aggctgctcc
    15601 gggtaccctc agggccctgt atgcccgcaa tgttggagaa aacgccgtac atggatcgga
    15661 ttcggatgaa aatgcagcca ttgaagcggc ttttcatttt gccgggacgg atctttattg
    15721 atgcccattt ctcttttgcc cctgttcatt ttctgaggtt tcttcggtca gagcctgaag
    15781 attctgtctt ttgaagccag ggacttcact cgttctttaa gttatccgct ttaattaagt
    15841 ctcatggcct gcctgtaagg aaccaaatgg cccctgtaag aaaccaaagg gccatggccc
    15901 aaaggataaa aaaacaaaag accaaccccg tatagggatc ggtcctgttt ttgaagccgg
    15961 gccccgatcg gagcccggtt gttctatctt aaagcgttgt ccgcttattg tgctccggca
    16021 gcggcatctt tcactttgat atccgaaacg atacgaatga aagactgggg aggattggta
    16081 ttggcagtaa ccgtgacctg tttggtttgg gcgcctgatt ttccggcgct gttgaattca
    16141 acgaccaatt caccggtttc gcccggggcg atgggttctg tcggtttttt cggaaccgta
    16201 cagccgcagg tggctttggc atcggtaatg accagcggtt cagctcccgt attcttaaat
    16261 ttaaaagtat gggatactac ctcaccctgg ttgatggtcc cgaaattgta ttcttcttcc
    16321 atgaactgaa tagtagtcgt tgggccggcg ggcacaatgg ccgcagcggc agaagcatta
    16381 gggtcggtgg tcgccgcagc ctctgtacct tcaatggcgg cacgggcttc gtcccggaca
    16441 gcgctatttt cattcttgca ggcggcgatc aagccaaaac ccacaaccaa caggaaaaat
    16501 agttttttca ttagtgtttg tttttaattt ttgataatcg aattgggtta aaattctata
    16561 caaaattagg aacttgggtt ttgcagtcca atactatatt gccaacgatt tgttaatggg
    16621 ccggtcgcca ttttaaagat tccggggttt cccttcaaac ggttttattg caaaatcaat
    16681 cctcttgaat agcctctttc aggaataagg accagggcct ggctgccgta tagtgtttca
    16741 taataaaggc tgcgaggtct ttgcgctgca ggtattccat cccagggtaa gtcgattcaa
    16801 aataaaattg tttgtgtgcc agcaagggtt ccgtccgggc cgcttcccgg aactccgggg
    16861 gtaaaatctt gctgatttcc cctttcatgg atccgaaaag agccagcagc tttttatcgc
    16921 ttttcaactg gccgacttcc ttcccctggt ggaggatggc agaccttatc ttttgcaatt
    16981 gactcttatc cggcatatat atcccgcaaa agatcaacaa acggtccgcc tccagctgga
    17041 tgtacatacc cggttcagat aaattcttcc ggcctccgtg ggcgatcaca gcggaaacat
    17101 aggtcttgta aggtgattta tccttactga aacgaatatc gcggttgatc ctgaataccg
    17161 cgtctttcac cgtacagagc attcccggct caagctgatg tacccggtaa ataagctccg
    17221 ctaccagggc ttcaaatggt ttcttcactt tctcttcata gacggttttg ttctcaagaa
    17281 accattcccg gccgttgtgt tttttcaaat tgcgaaggaa gcttgaaaaa ccaggatcga
    17341 aataagccat gggacgtatt tttgggtcca aattaaccaa agtcgtgaat taccaccctc
    17401 tcctcttttt agtatggcta tcgctgatga gcaattgtaa atccgatcaa atgtcaaaac
    17461 cacccatggt caatgaaacg attccggtcg tctatccggt caccgatacc tccaaccttt
    17521 tggaagatta ccacggctca gtggtcaacg atccttaccg ctggcttgag gtggacacag
    17581 ccaccgaggt ggaatcctgg gtaaaaagcc agaacctggt gacccaggac tatttgtcga
    17641 aaatcccttt ccgggagaac atccggaaac ggtatgaaga attgttcaat tataccaaac
    17701 tgagcgctcc gaggaaaatc ggggactatt atttttatta taaaaatgac ggccttcaga
    17761 accaggcggt catttattat caaaaaggcc gtgccggcag ggaggaagtc ttcattgatc
    17821 cgaataaact gtctgaaaaa ggcaccgtgt ccataaacct tctcggcgct tcccgggatg
    17881 accggtacct ggcctattcc cagcaggccg cgggatccga ctggcaggaa ataagagtca
    17941 gggagatcgc caccaatacc gaactccctg accggatcaa atgggtgaag ttcagtggcg
    18001 cggactggga ccatgagggt ttttattaca gccggtaccc ggatccgaaa ccgggcatgg
    18061 aattgtctga cgccaaccgg tttcattctg tctattatca tttactgggt acgggccagg
    18121 accgggacat caaagtatat gaaaacccgg acgaacctct catgaaccac agcgtccagg
    18181 tgaccgagga tggtaaatac ctgatccttt atgcctccaa agggaccagt ggaatggaaa
    18241 cccgattccg ggccgtgagc gataaaacca agtctttcca ggtcttgttt ccgggctttg
    18301 aaaacgaaag ttatgtcctg acccaccttg atggccagtt cattgccgtc accaatgtcc
    18361 atgcacccaa taaaaaagtc atccgaatcg accctggcca gcccggagag gccaactgga
    18421 aaaccctcat cccggaatcc ccccacctat tgaacagcct gagcaagggg ggtaaaaaac
    18481 tttttgcgga atacctggaa aatgccactt ccaaaatata ccagtttgaa tatgatggtt
    18541 cccaaaagac ggagattgaa ctgcccggcc tgggaagcgc tcatggattt gacggcaagg
    18601 aaggggacag cctccttttt taccagttta cttccttcac cgatccggga agcatctatg
    18661 aataccggcc ggaagagaaa aaatcaaagc ccttcttcca gccggaattg aagtttgacc
    18721 ctgccggttt tgaacaaaag caagtatggt ataaaagcaa ggacggcact tccataccca
    18781 tgttcctggt ttataaaaaa ggactggtgc tggacggaaa caatccctgt tatctctatg
    18841 cttatggggg ctttaacatc agcctttccc cttcattcag tgcttcccgg ctgatccttc
    18901 tcgaaaacgg gggcgtcttt gccatgccca atctgcgggg aggaggcgag tttggcgagg
    18961 catggcataa agccggaatg ctggagaaaa aacaaaacgt gtttgatgat tttattgcgg
    19021 cggcggagta cctcattgcg gagaaatata cttccaggga aaaactggcc attgcgggag
    19081 gatccaatgg agggctgctg gtcggcgcct gtatgaccca gcgtcccgac ctctttgccg
    19141 tggccttccc tgcagtagga gtgcttgaca tgctgagata ccataaattc accatcggcc
    19201 acgcctgggc cgtggaatac ggatccagcg acgtgaaaga acaatttgaa tacctgataa
    19261 aatattcccc cctgcataac ctcaaagccg gtacccggta tcccagtacc ctgatcacca
    19321 cagccgatca tgacgaccgg gtcgtaccgg cccattcctt caaattcgcc gccgcccttc
    19381 aaaaagcgca caaaggagaa aatcccacgc taatccggat tgaaaccgat gccggtcacg
    19441 gggctggcaa acccacctcc aaaatcatcg aagaacaagc cgatatctgg tctttcttct
    19501 tttacaatac caacagtccg gtgaaatata tgaagggata ggggtcagta gtcagtagtc
    19561 agtagtcagt agtcagtagt cagtagtcag tagtcagtaa tcaatggttg gtgaagagaa
    19621 gtggttcatc gtgggttttt gcgtatgccc gggagaaatc gagttttctc ctttgttttt
    19681 tcatgtaccc catagcctgg gtgagatgac ccacaaattc aggattggaa taatcggatt
    19741 tgtcatggcc cagggccgta atccaggcca ggcccccgtc aaaccggtgt tgccatacgg
    19801 aagggtaata ccgggcaaat ccccccttat tgagctcaaa gcggacggtc tccgctgaat
    19861 ccagtttcag cgactgcaaa tcggccacca gcagcacttc cggaccgggg tacaactcct
    19921 tcataaaata acattcatcc ttccgtaccc attttacagg gatggccttc acggagggat
    19981 ggtccggccg gatgttgagt acctcaaagg gctgaaatct cggatgccag gcaaaggtcc
    20041 cgccgagaaa ctgtttgaac caggtccagt tgcgttcggt acccagtacg gaatgaattc
    20101 ccagaaacaa tccaccggct tcgatatact gccggaaagc caggcgctgg gcgttggttt
    20161 caaagacttc attattggta ctggcaaaaa cgaccaggtc ggtcttggtt agttcttcgg
    20221 cagtgaaaac cccgggatct tcagtggctt tcagctgaaa gcgatgtttt cgcgcaagag
    20281 agtcaaaaca acggatggcc tgcgggatat tgtcgtgtac ataaccttta ccgtttttag
    20341 tgtacaacaa tacccttaag ccagagaggt cctgggctat cccgggagaa aataaaaaca
    20401 ggaggaacag gacaaggagc ttggttttca taaccggttt ttaaaactgt ttaactgaac
    20461 atacgattcc cacaagacaa tccctgcaca aacggcaata ttgagcgaat gtttgatccc
    20521 ggattggggt atttcgatgg cgctgtcaca caagccaagg gtggcttcgc tgaccccgga
    20581 gacttcgttt ccaaagacca ggaccagggg ctgtttttct gaaaagtcga actggtccag
    20641 ggggatgctg ccatggactt gttctacggc ggaaatatgg taaccctttt ctttcagatt
    20701 catcaaggcg gatgcaaggt cttccaccct ggcccaggga accgattccg tggcatccag
    20761 ggccgttttc aggatttctt tgtggggagg ggccacgctg aatccggcca gtaaaatttt
    20821 ttccatatta aaggcatccg ccgtccggaa tacggacccg atattcagtc ctgaacgtat
    20881 attttccagc accacggtga ccggtatctt ggcacctgct cgcatctccg ggaggccggg
    20941 cctgttgagt tccaacatgg tttttctgga aagcatcttt gcaaagatat gtcctatatt
    21001 cagcgcgtga aatttccgac cggggcaact ggcaaatatt taccatacct ggccctgtat
    21061 ttcatgcttc tgctggccca tggctaccag tatggatcag gggaccagag tgattttatg
    21121 ccttatgccc tgcacctccg gaatccggac ctgtaccccg gtgattttta tatttcctgc
    21181 ctgaaggaac acttcaatga acgatggctt attgccagga tcctgtccct gatccctcct
    21241 gccggctggc ccgtattatt tatgatcatt cattttttgg caagcgtgct cctgtttaat
    21301 ggattattga agttcagtgc ccactttctg tcccgggcct ggatgcaatg gctgctatta
    21361 tctgtcctcc ttatagtcct ctacccggtt catctgggag gcaacgaact gtattataat
    21421 atggtttgcc ccagtttgtt gtccaaagca gccgggatat ggtgtctgtg gttttgtttc
    21481 cggcaggatg accttaaggc cgccctttgc gccgttttgg ccacctggct ccatccggta
    21541 gccggtttcc aggtttttat cctgggcacg gcctttcttc ctgcggtaaa atggggaagg
    21601 tattgtatca tcacgcttac agcagtggcg ccctacttgt ttatccttct aaaaaatttg
    21661 accgggccgt cccaaatcct tccggaggtc atggctctcc ggaatgcgca tcatttttac
    21721 cctgcctatt tcggattgaa aaatgcgatc atcctgctgc ccatattttt cgcagggacg
    21781 gcacttgctt accggaagga tcgaagaatc ttcaggcttg ggatttgggt gctcctgggt
    21841 tgtggggtct acctccttac cctgtcggca tacccggacc tggctataaa aagtcaatgg
    21901 ttcaaatcca ccatttggct tgagtttttc tccctgctga tgatagggac ctggttatcc
    21961 gatcttaccc tgctccggga tgtactgaaa cgactgggga gccctttcct catgctggtg
    22021 tatctgatag ttcttgtcgt tgccctttac cgggcccggc ttgcgaactg gtactttcca
    22081 tggtcggccc ctaacgaatc gatacgcacc gctcttgaag ccaagtcggt gagtcagccc
    22141 ggggatcttt ttattgtgcc cgcagggttt acctccttta aattttttgc agaaaggccc
    22201 tgctggctgg actggaaggc cattccccac cggtcggatt gcctggaaga atgggccggc
    22261 agggtggaaa aagcctacgg cctcaccacc ggataccagg gaaatttgca ggagatcgac
    22321 cggatcagca atgcgcgttt ggccagtctg gaccaggcat caaaggaaat gctgaaagct
    22381 gccggggtaa aatacgtcct cttcagacct gagggaaaga cggattaccg gatgataaaa
    22441 ttatgaaggc atatagagta ccttgtcctt aagccgcagg taggccagca tatccatgtg
    22501 gtttccgtgt ttcattcctt ccaagcccat ccggtccaac agttgccggc aggcggtgcc
    22561 caggggttca tttgcataat cacggtcacg taaaaaatgg aggcatttca tcaacatgaa
    22621 cgcattcatg ccctggaaaa accggttccg gaaggtcttg aacgaaggac tgtgtttttt
    22681 catttctgag acccttgcct caaatccaat ggtatccaga aattcaatca atcctttttc
    22741 caggttcagc gagcggaaag acttctggaa atacaaggct tccaattggg tcaatagcgc
    22801 ccggatcact tcaaaattgc gcaaggcata ggtcatcatt tccacaggtg gcccatccat
    22861 ccgatcaagc aaggctttgc cggtcccaaa aggaacccgg tctgacaaac gggaggaagg
    22921 gtggacggtg gtccctccga ttgaagccac ctggtttttt accgagaatt tatgaataaa
    22981 ataaaaatcc tccccggctt gccttggggg catacccccc tccgaggcat aggccgccgc
    23041 atccacggcg aaagcggaac ccagggtttg gaatgcatac gggaatccgg catacctcag
    23101 ggcatggaca taataacgca ggtgcagctc gtagtccgta atcgcctcca ggtgtcgttt
    23161 tgagggcaag ttatccgtat cgtgttcata atggatcagc aggcaagcct ttgaagtatt
    23221 cctgaaggca tcccgcagga caaggaaata attggcctcc acccgggtat ccgcatcgag
    23281 gctgcagatc actcctccgg gccgcccggc tttaaaaaac cggaagcagg cctcatccat
    23341 gcctgttttt ctggccggcc ccaccccacc ttttttatga ggaagtccgg tcctgtccag
    23401 cacaaacagg gggcaggcac cggatctctt cggtaacgat tccaggctaa tccggttttg
    23461 cctcaccgcc gtttcggggg atttgtcggt atcgttgacc acgatcagga tctccacctt
    23521 ctgggaggca aaaccggccg cttcttcgat actttggaga cagggttcgg gatccgtttc
    23581 attaaatacc ggaatgacca cgataaaagc ggtgtccggg tccggcggct gattgaggag
    23641 cgggtgccgg gtttcctgcc tggaaaggta aaggccggtt ttgttcattc aggtgccgta
    23701 aaacggtaac ctacgccgtg gacattttca atttttatgg agggatcctt ttcaaaaaac
    23761 ttgcgcaacc tgctgacaaa aacatccatg cttcttccaa gaaaataatc atccttgccc
    23821 catacggcga ccaggatttc agaccgtttg ctcaactggt ttttgtgctt aaaaaaatag
    23881 gacagcaact cggcttcccg ctgggtcatg ccctgcttgc cctccgggcc ttcaatggta
    23941 taattttccg gatcgaaggt atatcggccg atggtgatga ccccgctggt gcggttgggg
    24001 tccacaaagc gccggcgcag gaagatttcg attcggagca ccagttcttc gatgctgaaa
    24061 ggtttggtaa tataatcgtc cgcaccaagg gaaaggccct cgatgcggtc ttctttcatg
    24121 gatttggccg tgaggaacaa tatgggcact tccttgcttt tggccctgat ttctttggcg
    24181 gcctcaaaac catccttcag gggcatcatg acatccagga tacaaaggtc cggtttttcg
    24241 gcttcaaagg ccttgatcgc ttccacccca ttgttgacga ggatgacctg gtaacccttg
    24301 gcattgaggt tatcggccgt aacgaaggca agggtttcat catcctccgc gagcaggatc
    24361 ttcgatttgt tcatgatatc actttgggta tctcaatata caaacttgta ccgacaccga
    24421 cctggctttc aatcctgatt ttccagccat gcgctttgca gatctggtat acgtaatgca
    24481 ggcccagtcc aaagcccttg acattatgta catttcctgt gggtacccgg taaaacctgg
    24541 aaaagatttt tttaaggtat tccttgggga tgccggatcc atggtcggca caacggatga
    24601 ccattttgtc cgcctcgtca taagtttcca attcgatttt cagctggtgg tcttcagagt
    24661 attttatggc attgtcgagc acgttctgaa tgatgtggat aaaatggatc cggtcggcgc
    24721 tgatgaccgg ggccatcgca ttcaactgga cgtggatttc cccgttttta gccttgatcc
    24781 tgggctctgt gccggaaacc aaatcggtga tgatttcatg gatattcacc ggttcaggca
    24841 ggatctgaaa attggttttt tccatccggg cgacctgcaa gaccctttcc acctgggtgt
    24901 tcaggagttg gttttgattc ttgatgatct gcgcgtattg gcgcagcctg ggattttcct
    24961 ggatgacttc gttttgcaac aggacatcgg aggagatatt gaccgcggca aggggagttt
    25021 tgaattcgtg cgtcaggttg ttgataaaat ccttttgcat ctcggccagg gttctttgtt
    25081 tcaaaatgat ccaaaggctg taggcaaaaa acagcagaat caacatcaac aggccaaggc
    25141 tgacccaggt atacgaataa aacgggttgg cctggcggtc cgggaagcgc actccgaaat
    25201 aatagagata atccttgtac gtggggagtt tggtacccgg acggatgttt ttctggtttg
    25261 cttcataact caccgcattg tgataaacca tgctgtcccg gtcgcagtcg tagatgccga
    25321 attcataatt ttccttcagg ccgacattct ggaattccac tttgagataa taatccaggg
    25381 tattgacatt gatgtggtcg ttgatattga ccaggaaata attggactgg ggatgtttga
    25441 tcaggctggt aaaactttcc aggtcgtcgt ttttcgacag ccgcttcgcc actttggaca
    25501 gggctgtgat ggccttgtcc cgaaaggccc tttcctccac gttctgttgc tgacggatga
    25561 tataaaattg aataaaaagg gtcgaaagga tggctatcga acccagaata atcaatcttg
    25621 caatgacgct gctcctcatg agaagacaaa gttaatcacc tccggggtat tgccgcctgc
    25681 ctgcttcggc gaagccaggc cgaaatgaat aatgcctatc ttgcctgccc atggatgatg
    25741 cgaccaccct ggtggccacc gaatattttc cgcgaatttc caccgtccgg cagatgatgc
    25801 tggcccaaaa ggtatggctg gcatacgggg aaacctatca gaaaaacagc ctgcgaaacc
    25861 gctgttacct ggcatccggc cagggtttgc agcgattgtc cgtgcccctt aggaaaggca
    25921 agcatcagtc ccagaaaatt tacaaggtca aaattgctta tgaagaggac tggcccttgc
    25981 aacatctgaa ggcgatggaa accaattacc ggtctgctcc cttctttctt catttcgtgg
    26041 atccggtccg cctcatcctg gaatccaggc ctgagtacct ggtcgatctg aattggaaaa
    26101 tcctgtcatt gcttttcaac gttttcaggt ggcatccgga catcgcctgt catatggaat
    26161 tcagttcccc ggctaagtca acgattagtc caacggtcaa actcaggcct tataaccagg
    26221 tatttgccgg ccggtttggc tttttagcgg atctcagtat cctggacctc ttgttctgca
    26281 tgggcccgga acccaaatat atcctcccct gaaaaatccg gacgttttat tggatattta
    26341 tattaaaaaa cagccatcgt gagtgttttc cagtttgatc ccaagattga agtttatcaa
    26401 agccttattg acgaagcggt taaaaacctg catgaactca cccaggaaat ccagaacaaa
    26461 gagctgatgg aaacggtgtc ctccctgagg gcaaggatcc gggatcccta tatgtttgtg
    26521 gtagtcggag aggtcaaggc gggtaagagc agttttatca acgccctgct cagcgccgga
    26581 cgggagatct gcaaggtggc cccgcagcct atgaccgaca ccatccagca aatcgtctat
    26641 ggcccggagg aacaggaagt gcatatcaat ccctatttca aaaggcattt tattccggcg
    26701 gaaatcctga aggaaattgc catcgtggac acgccgggca ccaataccat cgtagcccat
    26761 catcaggaaa tcacggaacg ttttattcct tcctcggatt tgatcgtatt tgtctttgaa
    26821 gccaaaaacc cataccggca gtccgcctgg gacctgtttg actttattaa ggacgaatgg
    26881 cgaaagaaaa taatttttgt cctgcagcaa aaagacctga tgtctccgga agacctggcg
    26941 gtcaacgaaa aaggcctggc cgagtatgcc tttaaaaaag ggcttccctc cgcaaaaata
    27001 ttttccgttt ccgccaaaga cgaactggag ggcagaactg accaaagcgg tttcaccctc
    27061 ctcagaaatt acatcaacgc aaacattctt cagggtaaag ccggatttat caaactctac
    27121 aacaatgtat ccaccgccca aaacatcaat tcccgcattt ataagggcat cctggaccgg
    27181 aaagaacaat tcctgtacga tctgaatttc agaaatgaca tccggaccac cctggacgag
    27241 caggaagcca agtccaaaca gcaggtggcg gtgttggcgg aaaacctgct tgcagcctat
    27301 gaccgggcca ctttaaaaac agaaaaagag ctggaagcgg gcctgggtat gttcaacatg
    27361 gtcaaacggt cgctgatctc tgtgttcagc aaaagccaat ccgtgaaaga atggctgacc
    27421 ggcctccttt ccggcctgga aaaagacctg cagatcgaat tgcagaacaa acttaccggg
    27481 ggcatcggag acattgcgga caatatccag caaatgggca aaatcattga cctgaagatc
    27541 cgcaacagca gaacggtact caaagacagc catgaactct ttgccgagat cgccgaacgg
    27601 cgcgccgggg tattccggga actgcaggaa acctttactt cttttctgaa ccggagtgaa
    27661 aatttcagtt cagcagacct cttccccggc caggaatccc tgtcgcctca gattgccgga
    27721 ggtacaggca tgggcatcgt cggcattgtc ctggccgcca ttgccaaagg caccattttc
    27781 gatgtgaccg gagggattct ttcaggtctt ggattcttgt ttgccggagc gctcgccttc
    27841 ttccaaaaaa gaaaatttat ggactccttc cgtaaagaaa tcaacaaggg aaggaacgac
    27901 ctcgattccg aggtaaaggc caggctgaac cagtatatcg taaacatcag gcaaaaaatt
    27961 gaacgttcct tcagcaattt tgacggcttg ctgcaatatg aagaaaaaca acttgcccgg
    28021 ctggacggct ttcacaataa aatcaaggat gacctttcgg gcctggaaac agaactccag
    28081 ggataccagc ttgcctgagg cctcaaaggc tttttttcat ctgccagtgg tccagtccca
    28141 cttctgtaaa ttttttcccc gcctttttat aacccaattt ttcataaaaa ggaatcgcct
    28201 gttccctggc gtgcagcacc atttccttaa aaccggccct cctgctccaa tcctcgctgt
    28261 aaccaaccag ctcccggccg aagcctgagt tttgtttttc aggcaatacc gcgacctggc
    28321 gcatccggac ctgtccgccc gtctccgggc tgaggaccag gcaggccagc aattggtaag
    28381 catcgtcata aagggccacg tgggtatctt caaattcttt ggaaaggtct tcttcactga
    28441 aatccagacc caatggttct ttcaataccc ggtaccggag cctgatcagc tcgtcgtatt
    28501 ccggggttcc gaaagcaacg gggtgaattt tcatttatgc ttattttagc caatattaat
    28561 caatccatat catgaaacca atattcgccg ccttactggc catgctgtac tttcacccat
    28621 tgagcgctca aagaatagcc aaagtaaaaa aggaagctgc cgcacccgtc gtgacggatc
    28681 tccgtccaat cgtggaccaa atggctaatg aaatagaaaa cgagatcatc gaagcgaggc
    28741 attggttgca tcagcatgcc gaattgagca accgtgaatt taaaaccgcg gagtacattg
    28801 ccaaatacct ggcctcggtg ggcatcgaac accagacggg tgttggtaaa accggggtgg
    28861 tcgggctgat aagaggggct aagccggggc cgaccattct gctccgtgcg gatattgacg
    28921 gacttccggt caccgagcgc gtggaccttc cctggaaatc caccaaccgg gacctttacc
    28981 tgggcatgca ggtcggagtc atgcatgcct gcggtcatga tacccatgcc gccatgctta
    29041 tgggcgccgc caaaatccta tacggcatcc gggaccagct gaaaggcaat atcaaactgg
    29101 tattccagcc tgccgaggaa ggccctcccc ccggagaaga gggaggcgcc agccttatga
    29161 tcaaggaagg attgatggag aatccagcgg tggacgtcgc ttttggcctg catatcaaca
    29221 gttctaccga agtgggccat atcaattata aacccggggg caccatggcg gccagcgatg
    29281 tcctcaggat aaaaatcaaa ggcaaacaaa ctcatgggtc aaccccatgg gacgggatcg
    29341 atccggtggt ggttggtgca caaattgtga tggcgcttca gacagtggtc agccggcaga
    29401 ccgaactgac caaggaggcc gccgtggtca cggtggccac ttttcacgcc ggggtt
//
LOCUS       DAOQXT010000055        29173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1875_length_29173_cov_6.510543, whole genome shotgun sequence.
ACCESSION   DAOQXT010000055 DAOQXT010000000
VERSION     DAOQXT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 29173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29173
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1875_length_29173_cov_6.510543"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..2587
                     /locus_tag="PKM27_10825"
     CDS             164..2587
                     /locus_tag="PKM27_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07797.1"
                     /translation="MISTYFKTAWRNLMKNKVFTAINIFGLSLGITAFLLIFNYLRFE
                     YSYDDFHQNKDLIYRVPMMIREDQGIEQTFAFTYPAVAPALKKDFPEIEHTVRFRRQG
                     GIVRHGEVKMVENGSIYFTDPSAFEVFSFSFYKGSAETAMRELNDAVITRETAERYFG
                     NEDPLGKALHYRNEDFIVKGVLDNLPSNSHIRFNILLNYNKYIQVTRGRAETSWGWSD
                     FYTYVKLKPGANPEALQAKLEAFAQRYLGDDMKKSAYSVNFVLQPLKDIHLRSRYDYE
                     LEGNGDLSYLKYLGIAALFILFIAWINYINLTTAHSMDRSKEVGVRKVIGAGKFQIIR
                     QFLAESFFMILAAALLGILGFRFGQPFFSKLVDKDITGFYETNFSFWALGLGVFLAGT
                     FLAAFYPAFILSSMPPAYSIKSAQPLPGGQGRGKNILRKSLVLLQFIAALALIAASLG
                     FYRQLRFMQSRDLGIQIDQTLVLQQNAALDSSDLPAYTAFVNTLEANPEIRSVTASTS
                     VPGGEVGGSADFVLQNSQAGKRCRLLGIDRKFMDAYGLELLGGRNFSTDRMSTDTAVP
                     VNILVNETAARVFGFSDPSGILGKEMDGGGFHCKVIGLVKDYHQESLQYGFDPIVFYP
                     AEESDMGNFSLKVITGKLPALMDFVKEAWNRHFPESPFQYYFLDEQFNEQYKNDRLFA
                     TVLGLFTLIAILLACLGLFGLSLFTIARRTREIGIRKVLGATLAQITRMITMDYLRLI
                     LLAGILALPLAFLLVNGWLKQYAFHISLGVWFFILPLVLLILITLITVSYQSVKAGLA
                     DPVKSLRSE"
     gene            2884..5298
                     /locus_tag="PKM27_10830"
     CDS             2884..5298
                     /locus_tag="PKM27_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07798.1"
                     /translation="MFKNYLKTALRNLLRNRAYALINIAGLSAGIAAALLIFLVIQYE
                     TSFDSFHKKKDVIYRIGTAFYLEGAYSYSEGTAFPVGPAVRNELAQVKNVARIFKTDD
                     EPVTIEDGAAVKRKFKADLYYTEPEFFDLLDFPWLAGDPHSVLSKPGQAALTVEMAEK
                     FFGGWQQALGKRIRKGATGQEIYTITGILENVPARTDFPLEVLISFVSIEHSPYRGNL
                     EDWVSTQGNAYTLVELPEDVSIPKINEDLKAFSLRHKPEEYAKDTYLLQPLSEIHYDA
                     EYGNFRNHTFSKSLITALQLIGFFLVLIACVNFINLATAQAVHRSKEVGVRKVLGSNR
                     NQLVIQFVQETAILTALAVILALGLAGTALPFLNGLLKTEMAFDLRNAPEIGFFLFVL
                     WLVVTLVSGSYPSVILSGFNPVTALKTKISSGKVGGINLRRGLVVFQFGIAHVLIIGT
                     LIVVSQMNYFRNARLGFDHHSILNITIPNDSVSRSRMDFVRNRFEQNPEIEKVSFSFS
                     SPAARGNWSSDFNFDHSDQSTSFNAILKWADAAFFETYNMGLAAGRMYFPGDTVKEFV
                     VNETLLRKLGISDPNQVLGKELEFWDGVLKGPVVGVVKDFNSLSLRQPMAPVVLSTWK
                     NQYRMLSLRIKPGEESDVIAYAEKVWNEAYPDYIFEYRFMDETLAGFYAQETQLSILY
                     KVFAALAILISCLGLFGLVSYMAVQRTKELGIRKVLGAGKANIIYLLSREFTLLILLA
                     FAVAAPLAYTLMQKWLQQYTYKIEINVWIFLLAMFASICIAWLTVGRVAWNAARANPI
                     RSLRTE"
     gene            5482..7932
                     /locus_tag="PKM27_10835"
     CDS             5482..7932
                     /locus_tag="PKM27_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07799.1"
                     /translation="MIANNFQFILRRMARNKLTTSLHLVGLSLGITVCLLIGLFIRHE
                     LSFDRYHTKADRIYRINQVWIDFGKKEFHYSTPFPLANQIRQDISGLERVTKVHHPFQ
                     AIIELNPLKRFKQDKVMFTDPEFLDVFDVEVVQGNAYETLRQPFHAVLTESTAKKYYG
                     NENPVGKVFLYNNKYNITVGAVIKDFPNNTHLPAELLLSLADDRDYVGTSLTHYGSVS
                     GGSTFILLPENNPEPTPVLLSGLIGIYDKVLNSQPWMGKDSRCEVELQPLKDIHFNSK
                     YANGGQWVQAVNKMWLWFFGGVGLAVLLLACINFVNLSTAESLGRAKEVGVRKTIGAG
                     KKQLIGQFLLESFGLVLISAMIGVLLTKSVLPYINDLTGKQIEFNILQSGGLLLYLFA
                     GILLTALLAGVYPALIIAAFRPILALKSVHPQKGLSTTLLRKGLVVVQFSISISLLIS
                     LLFIGKQLNFIRFKNLGFDKDNVIVVNLPNEQMKEKTLLTQELSRVPGLAAYSYSTSP
                     PGADQHWGTFMSVIGREDPDRKQVTTIMTDEHYCSLYNIKLIAGRTFIPSDTAAVSES
                     IPEDQRFAKTLVNQKAAAALGFESPEAALGKRFWAGMMGWHHEIIGVVEDFNVGSLRD
                     EIKPTLISPYLRFCDKLNVKVSGGTDINLVINQLGAAIRTAYPKGVFEYHFLDEQIDQ
                     LYKSESRLFGLFRLFSMIAMLISCLGLWGLITFTTQQKVKEIGIRKVLGASLSNIVSL
                     LTRDFILLVCISIAIASPLAYWGMNKWLQSFAFRVPLGWTVFLWAGVSTVLIVLVTVS
                     FQSIKGALANPVKSLRTE"
     gene            8024..10393
                     /locus_tag="PKM27_10840"
     CDS             8024..10393
                     /locus_tag="PKM27_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07800.1"
                     /translation="MLKNNLKLYLRSLVKNKSFSIINISGLAIGMAAAALILLWVQHE
                     YSFDQFHRNKDRLYQVWNRDVRNGKINCWSTTPKPMAPAIQKDYDEVEKTVRVNYDFP
                     ILFTNGEKRIKASCNIVDSGFLQMFTFPLLKGDVNSCLDQGTSVVIGKELAGKLFGTE
                     DPMGKTVKLDNQYLFTVTGVLDELPDNTQFGFECLIPWRFLVNIHQSDDYWPNNSTAT
                     YALLKPGVKVTAFQDKLKDLRKRYDQESPEMVTFLYPFTRARLHGVFVDGVETGGRIQ
                     IVRIFTAIAFLLLLIACINFMNLSTARSEKRAKEVGIRKVVGAQRISLVRQFLTESVL
                     ISFLSGCIAALIVYFSLPAFNELVGKKLQLDFGNPGFLLSASGFILLTGLMAGSYPAL
                     FLASFNPVRVLKGTYLKVQALVNPRKILVVSQFIFSIILIMATLIIRQQLLYAQNRQP
                     GYDRDRLVVHFFEGDMEKNYPLIKNELLQSGLAVSVTKTSAPITQGWSNTWGLSWQGK
                     DPDDRTLIDRFCADDAVVRTTGMELLVGRDFDLTKFPTDSTAALINESAARVMGFDRF
                     DQPIGQIVKDNDRDWHIVGVVKDFILHSPFQPTVPMFIAGAKGWFNVMHVRYTKDLPV
                     SQVMEGAEKIFKKYNPEYPFNYSFTDDQYERKFSDERRVSVLAGLFAGLTIFISCLGL
                     FGLVSFMAASRTRELGVRKVLGASAAHVIGLLSREFMMLVIIAFVIASPLAYWIMNKW
                     LENYTYRIDIHGWVFFVSGIAVVLISFATVSFQALKAAAANPIHSLRTE"
     gene            10471..12933
                     /locus_tag="PKM27_10845"
     CDS             10471..12933
                     /locus_tag="PKM27_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07801.1"
                     /translation="MLKNHLLLAFRNFWRNKVFSLINISGLAIGISAALVFYLIVQYE
                     FSFERFHPDGDRIYRVVSKIEFPDLTIHNSGVPVPTARAARREVSGLDGLTHFITVYD
                     TKVSIPIAGNASPVVFKNQKEIVYADEEYFKVFQYHWLAGSPHTALKDPFQVVLTESR
                     AKTYFAHLNYDEILGKTVVYDDSLKMTVAGIVRDIREATDFRFKEFISRATVEQTGLK
                     QHWSWEEWGSINSGSQLFVKLSPGTIPGVIEQQLASIRDKYRGNKDQEPKDGTQHFLQ
                     PLHDIHFNPEYDAFETRQANKSVLGGLMAVAAFLLLLGCINFINLTTAQSALRAKEIG
                     IRKTVGGRKGQLITQFLSETFLFTILAALLSLAITPGLLNIFKDFIPPGVSIQSISRP
                     HVWLFLVLLVTGVSLLSGFYPAWVLTGFKPVTVMKNQAHSGTSQTRKAWLRKTLTVSQ
                     FVITQVLLIATLAFSKQIRYSLNKDLGYQKEAIVYINTEWNIFSNAKDDRRFILREKL
                     KRIPEIERVSLGSSPPASNNTSTMTMKFKNGDKVVETMVEVKYADADYFDLYKMKLIA
                     GKPLQESDTTRQFVINETYARMLGFDKPEEAIGHFIDRNHPTPVVGVVADFHTKSTHA
                     PIKPLAYSSEMNSSYTLHMALKPGGKEAGSWKKALDETEKIYRELYPEDDYKYTFFDE
                     SIARFYTSEQNIIRLLKWAAGLCVFISCLGLLGLVIYITNQRTKEIGVRKVLGASVVQ
                     ILALLSKDFVVLVVLAFFISAPLAWWGVHRWLENFAFRVEIGWLIFFVVGLSAVLLAL
                     FTISFQSVRAAMANPVESLRTE"
     gene            13227..15683
                     /locus_tag="PKM27_10850"
     CDS             13227..15683
                     /locus_tag="PKM27_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07802.1"
                     /translation="MWSNYLKVALRNLWKHRSFSAINIAGLAIGMAACLLILQYVSFK
                     LSFDQFNVHLKDIYRVVNDRYQNGKLIQHGTITYSGVGKAMNDDYEEVIRNARVVPSG
                     ETIISYGDKKLAEDQVLYVDEAFFSVFSYPLLAGDASTTFQEPYAVVLSESLAGKLFN
                     YKGNEFQQFIGKQISLLPDTMLYQVEAICKDVPEHSHLNFELLISYPTLISNGWEEAG
                     YNFTQSDFWHYVQLTPGTDYLKLNEKMPAFSQTHFQGNKVSGSDEKFYLQPLGKAHLY
                     SDFEYEIGRTGSATVVWGLLLIALFIIGIAWINYINLATARAVDRAKEVGVRKVAGST
                     KGQLITQFLTESLLVNGLGILLALLIVLLVQRPFNTLLEHKLSLVYLFSKGMSGYGIP
                     IGLGILVLTGVAVSGFYPAFVLSSFKPISVLKGKFQSSRQGILFRKGLVIGQFSITIA
                     LIIGSIIVMRQLRYMNRASPGFDMDQVMIVQSPSLTSWDTAFISRVNNFKQELKRLVQ
                     VKGATTSWNLPGGDIGRSFNVRRADSSGTDRYTMRHTSVDFDFVPMFSIPVLAGRSFR
                     TEDHHWDGQRLQNIMINASAARMLGFESPEAAVGKSIYRGQKKWEVIGVLGDYHQKSL
                     RFPLEPMLFLPYYSTNSDIAVKLNPSGNFSQTISLIREKYEAFFPGNLFDYTFLDEVF
                     NRQYQSDRLFGRVFGIFAGLAIGIACLGLLGLTMFSTVQRTREIGVRKVLGASVASIL
                     ILISRDFIKLVLIAAFLAFPIAGWVMKEWLDDFAFRINMGWEVFIIAGSGALLIALFT
                     ISYQAIKAARANPVKALRTE"
     gene            16022..18424
                     /locus_tag="PKM27_10855"
     CDS             16022..18424
                     /locus_tag="PKM27_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07803.1"
                     /translation="MFWSYFIIAWRNLKNHKGYSLINIGGLAVGMAVAMLIGFWIYDE
                     LSYNKYHQNYDHIGQVMQHQTFNGHKGTESSIPIPLGTELKNKFGSDFKYLVMASWQG
                     DHILSFGEKHLTREGNYMDVDIARLLTLEMIQGTPDGLKDPNSIFLSESTSKALFGDE
                     EAMGRKLKIDNKLDVAVTGVYKDLPFKTEFRELKFIAPWELYVSSMEWVKQARNENQW
                     GNNSFQLFAQIADQADMEKLSEKIKMAKQNQVPEDEKKFMAEIFLHPMADWRLRSNWV
                     EGVKTGGFIQYVWLFAGVGLFVLLLACINFMNLSTARCERRAREVGIRKSLGSERAQL
                     IRQFFSESLLVVLIAFLAALGLILGALPLFNEITNKQMVLPAGNLSFWLVSAAFIALT
                     GLLAGSYPALYLSSFQPVKVLKGTFRVGRLAALPRKILVVLQFTVSITMIIGTVLVYQ
                     QVLHTKNRPLGYDNNGMIMTQMKSIDFYGKLDLLRNELINNGAILEMSESSSPLTGIW
                     SNNGGFNWEGKDPGLQAEFATVWVTPEFGSSIGWKIREGRDFSRDFSTDSQAMVINTA
                     AVRFMNIDQPLGKVIRWGDDQNARTYTIIGVIDDMLMQSPYEPVKQTIYFMNPERANW
                     MNFKLNPAKSVDESIGKIEAAFKKYIPSAPFDYTFVDEEFGKKFETEERVARLAALFA
                     ILAVFISCLGLFGLSSFVAEQKTKEIGVRKVLGASVANLWALMSRDFTRLVILSCIIS
                     IPLSYYVLNGWLLNYTYRTSFSWWIFAIGGAGALFIALVTVSFQAIKAALANPVKSLR
                     TE"
     gene            18438..20864
                     /locus_tag="PKM27_10860"
     CDS             18438..20864
                     /locus_tag="PKM27_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07804.1"
                     /translation="MFSNYFKTAWRNLLKYKTYSGINILGLATGLAAFILISTYVRQE
                     LSFDRFNAKADRIYRLNTEIKFGDNHLDLAVCSPLVGETILRELPSVEQVARLRWYGE
                     FLVKKEDENVKETNVAWADASLFEVFSLPMIAGDPETALKDPGSIVLTETVARKYFNS
                     TDIIGKILTVNNREQKKITGVIRDLPNTCHFQFTSFLPNADDPSSREDTWAGSQNWNT
                     YVLLKPGTALDQFANDINRVYDKFLEPQLQKVINKSLSEFNASGDFFKLTLTKLTDIH
                     LRSSRAGELYGSGSLQNVYIFSAIALFILLIASINFMNLSTARSSNRAREVGVRKVLG
                     SSRPALISQFISESFLTVALALILAVCIGYLALPYFNTLVGAGIDRESLISAGMVLPG
                     LALILGVGLLAGSYPAFYLSSFIPVKVLKSLKGTAMNRSLLRNALVVFQFSTSVVLIA
                     GTLIVYRQMDFIRKKDIGYNRDQMLIINNTSHLGNKIDAFRLDLLRLNGVDQAAVSGF
                     LPVNYHRTSDSYFPDASLSTRDAISMQSWGIDEYYIPALEMKILKGRNFSPDRKSDSL
                     GLILNESAARFFGAGEVIGKKLYQVKDESTKELAIYEIIGVVKDFNYSTFRDQVQPLC
                     FTFEKDPGSLSLRMQTANIPDLLAEIKTLWKSYLPSLPFEYYFMDDEYNRRLEGEQKT
                     GELFSFFAMLAIFIACLGLFGLSAYMAEQRAKEIGIRKVLGASVSGITQMLSRDFLKL
                     ILISIVLAVPVAYFLMNRWLQEFSYRVGIQAWVFLLTAGLILLIAVLTVSFQAVKAAL
                     ANPVKSLRTE"
     gene            21104..23557
                     /locus_tag="PKM27_10865"
     CDS             21104..23557
                     /locus_tag="PKM27_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07805.1"
                     /translation="MLNNYLILAWRNLMKRKGFSLINSLGLAAGIAICMLIVLFIRDE
                     LSFDRFHPNGDRVYRMVVDRKYPGRSTSYSMIPQSYSQAVKQECPEVEATTRVFDFLG
                     GSAAQYRYGEKKFEEFRVLQVDSTFFEVFAGEFVSGDPATALDQPNSVVMNESTAARY
                     FGSADEALGKNIQPEGDNNQPLRVTGVCKDWPSHSHFDFDLLRTTAGNAFAARENFVG
                     FAAHTYFLLKLNTSPGQVEQKMPDIIGKYAAGDIARQFSVPFEQFQAAGNGYRYYLQA
                     LTDIHLDSRLEGELKPNGSRVAVYAFGIIALFILVLAIINFVNLSTARSGERAREVGI
                     RKTFGSGKKSLMFQFLSEAVLMGLASLIIAAGLVYLILPFFNQLSGKAFTMNNLLTGP
                     NILLFSGLALGTGLLAGCYPAFVLASFRPVQVLKGKLQSSQYGLFLRNGLVVFQFSIS
                     MVLIICTLMVNRQMNYMTSNSLGYNKDYTVLIQRTDLLGPQTQAFKNALLQVPGVKNV
                     SGASAFPGDPNYFGISWRQVGSNEPMTGRGILTDDQYQSLFELELVEGRFFSKDYGTD
                     SLAVVLNEAAVKELGLVNPIGARLISDEDFLNGPDNQSYEYHVIGILKDYNYQNLHLP
                     IVPLVFTSTSRFQDVTFSAAVKLHGQEMQAALRSIENEWKKFVKDRPFHYEFLDQSIA
                     RQYEAETRTRSIFSFFAGLAIFIACMGLLGLSAYATQQRMKEISMRKVLGASTLGIVS
                     LLTKDFMKLIGLAILLAFPLAWYGMSRWLQGFSYRAELSWWIFGLAAAGTLCIALVTL
                     GAQAYRAGRLNLIRVLRTE"
     gene            24186..26564
                     /locus_tag="PKM27_10870"
     CDS             24186..26564
                     /locus_tag="PKM27_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07806.1"
                     /translation="MFKNYLVVTWRILIKNKMYSAINLAGLAIGFACCVALGMYIYDE
                     WSYDRFHFHKDNIYRVVANIKQTGGTFDMAVTPGPLAPALKADFPEVIQTCRIARQRN
                     AVMEIRDQVFEPAQTLSVDHSFFELFDFKLLKGNKATVLLEPDEAIISEKLAIQCFGK
                     DWYLQDALLGSTFKFNDHRLLTIAGIIQDPPDQSHLQFDLLLSFKNEEISNGGYNWDS
                     NNYHTYVLLGPQADLARLQDKLLGFLESKNKYLEGNTLSLQPLLDIHLRSDFAFQTDW
                     AKTSNRVYIRIFFAVGLIVLFIALFNFINLSTAKAVERMKEVGVRKAIGAARGQLITQ
                     FFVESLTLSFLAIFISYALLLGFIPILNSVADKSLSIPYTLNFLLVLTGFSFVSGLLA
                     GIYPAIYLSGFKPVKVLKGIFDLNSSRFRQVLVISQFTPSIILIIVTIVIHHQINYLQ
                     NKDLGFDQSQLLSLKVKTKEIAPVNLMKQEMIAQPGIASVAATSSTLIDVINSTGRVK
                     WQGQGADEKLMFTTINIDPDYLTTSGMHLIAGRNFDPGRPVDNTKSFIINETAASKLG
                     WTPEEAVGKSFTIWDSTGNIIGVTRDFHYKPLTSSIGAFVFRYWPAESFQYLLIKIKP
                     NHIREAISNIEKVYKKFDPKTNIQYEFVNELVEHQYRTQLRSGKIILYFSILTIFVSC
                     LGLFGLVTFTTVQKTRQIGIRKVLGARVVSLVRLLSLDFIKLVGIAMVLAVPASIWLA
                     NRWLQDFAYKIELKWWMIGGAGLLALAFSVLTVSLQTLKAAMANPVKSLRSE"
     gene            26628..29051
                     /locus_tag="PKM27_10875"
     CDS             26628..29051
                     /locus_tag="PKM27_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07807.1"
                     /translation="MLKNYILIAWRNLIKHSSYTAIHILGLGIGFVAAFLIVLFIRDE
                     LSYDRFHTNIDDLYRLNFYGKMGDQEAHTSQAPGPAGPLFQSLFPEVEAACRIKTAGD
                     FSVRVGDKAYRESRLAYADSSLFRLFTFAVREGSTSGALNRPGQVIINATTAKKYFGA
                     ENALGKTLVLDKIKTYTVSAVIEDLPGNSHFQFDFFLPMAENQDSYVENWGSTNYHTY
                     FLLKPGTRVEALSSKMNESFREKFNLVLKQYLNSSLEEFTAQGNYARVELFPVRKIHL
                     YSNLDDELAHNGNISLIYIFGLAGFLILLLAGINFVNLSTARASVRSREVGVRKSIGA
                     LNRDLTLQFLAESMLVCLLCWLMAMILVSVSLPWFNNLAGKSLEIGGLFNAEVLIPLV
                     IIALVTGFTAGIYPALFLSALEPVKVLKGTLGLGLRKSKLRSGLVIFQFFVSIVLIIG
                     SLSIYRQVEYIQNKKLGYTKEQVLVISDAYLLGNQLEAFKNRLKAMPPVKNLSVGAFL
                     PVSSERNTTSIVLGNVPSPGNTFLVNNWWTDPDFLKTLEIELVQGRDFFRAFPADSQA
                     VIINETLARFFGYPGENLEGQVIGLPRDSGKIVPYHLIGVVRDFNFLSLHHPIEPLAI
                     FYGGTPDFITIRAETGQIQELLREIESGWNQLARGSPFTYDFLDDRYRQLYGAETKMG
                     KIAFVFAFLAIFIASMGLLGLVAFTIQQRFKEIGIRKVLGASLPDLLSLVSKDFILLI
                     GAAILFACPLSWWLVQQWLKRFVFRMDPGPGLFIGTALGTILIALVIVGLQTLKAARV
                     NPVKSLRTE"
BASE COUNT         7234 a         6992 c         7229 g         7718 t
ORIGIN      
        1 cataccatcc tccatatagg attaccctct acctgtacaa tcaatatttg tagggtccca
       61 tttggcatct ttatccaaaa aactaaagct gaaaaggctg tagtgcaaca agcacagtaa
      121 tcagtaatca gcaaaccaat caacatccac cacccacctt cccatgataa gtacatactt
      181 caaaaccgcc tggagaaacc tgatgaagaa caaagtgttc acggcgatca atatattcgg
      241 cctcagcctg gggatcacgg ccttcctcct catcttcaat tacctgaggt ttgaatacag
      301 ttatgacgat tttcatcaaa acaaggacct gatctaccgg gtgcccatga tgatccggga
      361 ggaccagggg attgagcaga ccttcgcgtt tacttacccg gcagtggctc cggctttaaa
      421 aaaggatttc ccggaaatcg aacatacggt gcgcttccgc cggcagggag gaattgtgag
      481 gcatggagag gttaaaatgg ttgaaaacgg cagcatttat ttcacggatc cttccgcctt
      541 tgaggtcttt tccttttcct tttataaagg atccgcagag accgcgatgc gggaactgaa
      601 tgatgcggtc atcacccggg aaaccgccga aaggtatttt ggcaatgagg accccctggg
      661 caaggccctg cattaccgca acgaggactt tatcgtaaaa ggggtgctgg acaacctccc
      721 ctcgaattcc catattcgtt tcaacatact gctcaattac aataaataca tccaggtcac
      781 gaggggcagg gctgaaacca gttggggctg gtcggacttc tatacctatg taaagctaaa
      841 acccggtgca aacccggaag ccctgcaggc gaaactggaa gcctttgccc aacgatacct
      901 gggggacgat atgaaaaaaa gcgcctattc ggtaaatttt gtccttcagc ctttgaaaga
      961 catccatctc cgctcccggt atgactatga actggaaggc aacggggacc tctcctacct
     1021 caaatacctg ggcatcgccg ccttgtttat cctcttcatt gcctggatca attacatcaa
     1081 tctgaccacc gcgcattcca tggaccggtc caaggaagtg ggtgtgagaa aagtgatcgg
     1141 cgccggaaaa tttcaaatca tccgtcaatt tcttgctgaa tcttttttta tgattttagc
     1201 tgcggccctg ctgggaattt tgggtttccg gtttggtcaa ccgttttttt caaagctggt
     1261 ggacaaggat atcaccggct tttacgagac caacttctcc ttttgggccc ttggcctggg
     1321 cgtctttctg gccggtactt ttctggctgc cttttacccg gcattcattt tgtcctcgat
     1381 gccgccagcc tattccatca aatcggccca gcccttgccc gggggtcagg gcagaggaaa
     1441 aaatattttg cgtaaatccc tggtcctcct tcaatttatc gccgccctgg cgctgatcgc
     1501 cgcctcgctg ggtttttacc ggcaattgag gtttatgcaa agccgcgatc tgggtattca
     1561 gattgatcaa accctggttt tgcagcaaaa tgccgccctg gatagttcgg acctgccggc
     1621 ttataccgct tttgtaaaca ccctggaagc caatccggag atccggtcgg tgactgcgtc
     1681 cacctcggtt ccgggaggtg aggtcggagg ctccgccgat tttgtgctgc aaaacagcca
     1741 ggccggaaaa agatgccggc tgctgggcat tgaccgcaaa ttcatggatg cttacggtct
     1801 tgagctgctc ggagggcgga atttttcgac agaccggatg agcaccgaca cggcagtgcc
     1861 cgttaatatc ctggtgaatg aaacggccgc aagggttttt gggttttcgg acccgtcggg
     1921 gatcctgggc aaagaaatgg acgggggcgg ctttcattgt aaggtcatcg gcctcgtgaa
     1981 agactatcac caggaatccc ttcaatatgg ttttgacccg atcgtctttt atcccgcgga
     2041 ggaaagcgat atgggcaatt tttccctgaa ggtcattacg ggaaaactgc ctgcgctgat
     2101 ggattttgta aaggaggcct ggaaccgaca ttttccggaa agtcccttcc agtattattt
     2161 cctggatgaa caattcaatg agcaatataa gaatgaccgc ttgttcgcga ccgtactcgg
     2221 cctgtttacc ctgatcgcca tcttgcttgc ctgcctgggc ctttttggtt tatccctgtt
     2281 taccatcgcg cgtcgcacac gggaaatcgg gatccgaaaa gtactgggtg ccacactggc
     2341 gcaaatcacc aggatgatca ccatggatta tctccgcctc atcctgctgg ccggtatcct
     2401 ggcgcttccg cttgccttcc tgctggtcaa cggctggctg aagcagtatg cttttcacat
     2461 ttcgctgggt gtctggtttt ttatactgcc cctggtcttg ctgatcctga ttaccctgat
     2521 cacggtcagt tatcaatcgg tgaaggcggg attggcggat ccggtgaaat cgctgaggag
     2581 tgagtgagac agttagcaag ttagccagtt agccagttaa ccagttaacc agttactgat
     2641 aaactgataa actgctatac tggtatactg ataaactaaa aaaccgatac accattgtaa
     2701 cggaaccgga cacttgaagt catttatttt ttcttaatct attgataatc agaaggtgta
     2761 acctttggta tgggaaatgg gtataaggag gaaggaagga ggaaggaaag gggtggactg
     2821 cttatagctg accactgaaa tctgaaatct gtctccatca ttaatcaaat aattcgccaa
     2881 tccatgttta aaaactatct taaaacggct cttagaaacc tgctgcgtaa ccgcgcctat
     2941 gcattgatca atattgccgg attgtcggcc ggcattgccg ctgcgctgct tatcttcctg
     3001 gtaatccaat atgaaacgag tttcgatagt tttcataaga aaaaggatgt gatctatcgc
     3061 atcgggactg ccttttacct ggaaggggct tattcttatt cggagggaac agcttttccc
     3121 gtggggccgg cggtacggaa tgaactggcc caggttaaaa atgtggcgcg catttttaaa
     3181 acggacgatg aaccggtgac cattgaagac ggggctgccg ttaaacggaa attcaaggcg
     3241 gacctgtatt atacggagcc ggaatttttt gacctcctgg attttccatg gttggccgga
     3301 gatccccatt cggtcttatc caagcccgga caggctgccc tgactgtgga aatggctgaa
     3361 aaattttttg gaggctggca acaggccctg ggcaaaagga taaggaaagg agctactggg
     3421 caagaaatct ataccatcac cggtattttg gaaaatgtgc cggccagaac cgatttcccg
     3481 ctggaagtcc tgatctcttt tgtttccatt gaacatagcc cgtacagagg caacctggag
     3541 gattgggtga gcacccaggg aaatgcgtat accctggttg aactcccgga agacgtatcg
     3601 atacccaaaa taaatgaaga cctaaaagca ttttccttaa ggcataaacc ggaagaatat
     3661 gccaaagaca cttatctcct gcagcctttg tcggaaattc attatgatgc cgagtacggc
     3721 aatttcagga atcatacctt cagcaaatcc ctgatcacgg cccttcaact gatcgggttc
     3781 tttctcgtcc tcatcgcctg tgtcaatttt atcaacctgg ctacggccca ggcggtccac
     3841 cgctccaaag aagtcggggt gagaaaagtt ctgggaagca atcgcaacca gctggtgatt
     3901 cagtttgtgc aggaaacggc gatcctgacc gccctggcag tcatcctggc ccttggcctg
     3961 gccggtacag ccctgccttt tttgaacgga ctccttaaaa cggaaatggc ctttgatctc
     4021 cggaatgccc ctgaaatcgg ttttttcctt tttgttttat ggctggtggt caccctggtg
     4081 tccggcagct atccgtctgt catcctttcc gggtttaacc cggtgaccgc cctcaagaca
     4141 aaaatctcat ccggaaaagt gggtggcatc aacctcaggc gcggcctggt ggtcttccag
     4201 tttggcatcg cccatgtatt gatcatcggt accctgatcg tggtcagcca gatgaattat
     4261 ttccgcaatg cccggcttgg atttgaccat cactcgatcc tcaatattac catccccaac
     4321 gatagcgtca gcagatcaag aatggatttc gtgcggaacc gcttcgaaca aaacccggaa
     4381 attgaaaaag tcagtttcag cttttccagc ccggcggcta ggggaaactg gtccagcgat
     4441 tttaatttcg atcactcaga ccagtccacc agtttcaacg ccatcctcaa atgggccgat
     4501 gcggcctttt ttgaaacgta taatatggga ctggcagccg gcaggatgta ttttcccggc
     4561 gatacggtaa aggaatttgt ggtcaatgaa acccttttgc gtaaactggg catctcggat
     4621 cccaaccagg tgcttggtaa ggaacttgaa ttctgggatg gagtattgaa aggcccggtg
     4681 gtgggtgtcg tgaaggattt taattccctt tctctccggc aacccatggc cccggtggtc
     4741 ctcagcacct ggaaaaatca gtaccggatg ctcagtctcc ggataaaacc cggagaggag
     4801 tccgatgtga ttgcctacgc tgaaaaagtc tggaatgaag cctatcccga ttatattttt
     4861 gaataccgtt ttatggacga aacgctcgcc gggttctatg cccaggaaac ccaactgtcc
     4921 atcctgtata aggtctttgc cgccctggcc atcctcatca gctgcctggg tttgttcggc
     4981 ctggtttcat acatggccgt tcagcggacc aaggaactgg gcatccggaa agtcctgggc
     5041 gccggcaagg cgaacatcat ctacctcctg tccagggagt ttaccctgct gattttgctg
     5101 gcctttgcgg tggctgctcc gctcgcctat accctgatgc aaaagtggtt gcagcaatac
     5161 acctataaaa tagagatcaa cgtctggatt ttcctgctgg ccatgtttgc ctcgatatgc
     5221 atcgcctggc tcaccgtggg gagggtcgcc tggaatgcgg cccgcgccaa tcccatccgg
     5281 agtctgcgaa cggaataaac aggggatttt agattaacgg gagctccggt ccacaccggt
     5341 cagcccgttt aaaatcaacc atattttgtc cgaaaacgga cacggaggtc aagggttatt
     5401 tatttaagtg gcagaaaatc aactattaac cattttggca caggaatagt cgaattaaat
     5461 caaaccatct actttttgcg tatgatagcc aataattttc agtttatact tcgccggatg
     5521 gcccgcaaca agctgaccac ctcgcttcat ttggtgggat tgagcctggg catcacggta
     5581 tgcctgctga tcggcctgtt tatccgccac gaattgagtt ttgaccggta tcacaccaaa
     5641 gcggacagga tctaccggat caaccaggtg tggattgatt ttggaaaaaa ggaatttcat
     5701 tattcaactc cctttccgct ggccaaccag atccgccagg acatttccgg tctcgaacga
     5761 gtgacgaagg tgcatcatcc gttccaggcc atcattgaat taaacccgct caaacgattt
     5821 aagcaggaca aagtcatgtt tacggaccct gaatttctgg acgtgttcga tgtcgaggtg
     5881 gttcagggca atgcttatga aaccctgcgc cagccttttc atgcggtttt aaccgaatcg
     5941 acggcgaaaa agtattatgg aaatgaaaac ccggtcggaa aagtgttttt gtacaacaat
     6001 aaatacaata ttactgttgg agccgtcatt aaagattttc ccaacaatac ccatttacct
     6061 gcagaattat tattatccct ggctgatgac cgggattatg tggggacctc cctgacccac
     6121 tatggttcgg tctccggggg gtctactttt attttattgc cggagaacaa ccctgagccc
     6181 actcccgtct tgctcagcgg tctgattggc atttatgaca aagtcctgaa cagtcaaccc
     6241 tggatgggta aagattcccg gtgcgaagtg gaacttcagc ccctgaagga tatccatttc
     6301 aattccaagt acgcaaacgg aggacaatgg gtgcaggccg taaataaaat gtggttgtgg
     6361 ttttttggcg gggtgggcct tgccgtgctt ttgctggcct gtatcaattt tgtcaattta
     6421 tccaccgcag agtctttggg gagggcaaag gaagtggggg tccgcaagac cataggcgcc
     6481 ggtaaaaaac aattgatcgg tcagtttctt ttggaatcct tcgggcttgt gctgatctct
     6541 gcaatgatcg gggtgttgct taccaaatcg gtactgccct acatcaatga cctgaccgga
     6601 aaacaaattg aatttaacat cctgcaatcc gggggattgt tgttgtattt gtttgcgggc
     6661 atcctgctga ccgctctcct ggccggggta tatccggcct tgatcattgc cgccttccgg
     6721 cccatcctgg cgcttaaatc cgtgcatccg caaaagggtc tttcgaccac cttattgaga
     6781 aaagggctgg tggtcgttca attcagtatt tcgatcagcc ttttgatcag cctcttgttc
     6841 attggcaaac aactcaattt tatccgcttt aaaaacctgg gtttcgataa ggacaacgtg
     6901 atcgtggtca acctgcccaa tgaacaaatg aaggagaaaa cattattaac ccaggaatta
     6961 tccagagtgc ctggcctggc cgcttattcc tattctacca gtccacccgg ggcagaccaa
     7021 cactggggga ccttcatgag cgtgatcgga agggaagatc cggatcggaa acaagtgacc
     7081 accatcatga cggatgagca ttactgctcc ctttataata taaaattaat tgccggaaga
     7141 acatttattc cctcagatac cgcggcggta tcggaatcca ttcctgaaga tcaaaggttt
     7201 gccaagaccc tggtcaatca aaaagcggcg gcggccctgg ggtttgaaag tccggaagcc
     7261 gctttgggca aacgattttg ggccggaatg atgggctggc atcatgaaat catcggagtg
     7321 gtggaagatt ttaatgtggg ttcgctccgg gacgaaataa aaccaacact gataagccca
     7381 taccttcggt tctgtgataa actgaatgta aaggtcagcg gcggtacaga cataaacctg
     7441 gtcattaatc aactcggagc ggcaatcagg accgcatatc ccaaaggagt ttttgaatac
     7501 catttcctcg atgaacaaat cgaccaatta tataagtctg aatcgaggtt gttcggcttg
     7561 ttccgcttgt tttcgatgat cgccatgttg atttcctgcc tcggtctgtg gggcctgatc
     7621 acctttacca cccagcaaaa agtaaaagaa atagggatcc ggaaggtttt aggcgcctcc
     7681 ctgtccaata ttgtgtccct gcttaccagg gactttattt tgctggtttg tatttcgatc
     7741 gcgattgctt ctcctttggc ctattggggc atgaacaaat ggttgcaatc cttcgccttc
     7801 cgggttcccc tcggatggac cgtattcctc tgggcaggcg tgtctacggt attgattgtt
     7861 ttggttacgg tcagttttca atcgatcaaa ggagcattgg caaatccggt gaaatccctg
     7921 aggacggaat gagatgcgag atgcgagaca cgagatggca acagtttaca aactatgccc
     7981 gaactgatcg ggactccaat caccattcat caaccgccaa gccatgttaa aaaataatct
     8041 aaaactctat ttgcggagtt tagtcaagaa caagtcgttt tccattatta atatctccgg
     8101 tctggcgatt ggcatggccg cagcagcttt gatcctgctc tgggttcagc atgaatatag
     8161 ttttgaccag tttcacagaa ataaagaccg gttataccag gtttggaacc gggatgtccg
     8221 gaatggcaag atcaattgct ggagtaccac cccaaagccc atggcgccgg ccattcaaaa
     8281 ggattatgac gaagtggaaa aaacggtcag ggtcaattat gatttcccca ttttatttac
     8341 taatggagaa aagaggatta aagcttcctg caatatcgtt gactctggct ttttgcaaat
     8401 gtttactttt cctttgctaa agggagatgt aaacagttgc ctggaccagg gtacatcggt
     8461 tgtaatcggg aaagaactgg cgggtaaatt attcgggacg gaagatccta tgggaaaaac
     8521 cgtcaagctg gataatcaat acctgtttac cgtgaccggt gtcctggatg aattgcccga
     8581 taacacccag tttggattcg agtgtttgat tccctggagg ttcctggtca atattcacca
     8641 atccgatgac tattggccca ataattccac cgccacctat gcccttttaa aaccgggtgt
     8701 aaaggtcact gcgttccagg ataaactgaa ggatttgcgc aagcggtacg accaggaaag
     8761 cccggaaatg gttactttct tatatccttt cacccgggcc cggttgcacg gcgtttttgt
     8821 ggatggagtg gagaccggtg gaaggataca gattgtccgg atattcacag ccattgcttt
     8881 tctattgctg ctgatcgcct gcatcaattt tatgaacctc agtaccgcgc gaagcgaaaa
     8941 acgggcaaaa gaagtgggca ttcgtaaagt ggtcggagcg caaaggattt ctctggtccg
     9001 ccaattcctg accgaatctg ttttaatctc ctttctttcc ggttgcattg ctgccttgat
     9061 tgtatatttt tctctgccgg catttaatga actggtcgga aaaaaattgc aactggattt
     9121 tgggaatcct gggtttctgt tgtccgcatc aggttttata ttattgaccg gcttaatggc
     9181 aggttcttat ccggctttat tccttgcttc attcaacccg gtgagggtat tgaaaggaac
     9241 ttacctgaag gtgcaggctt tggtgaaccc gcgaaaaatc ctggtagtga gccagtttat
     9301 tttttccata atactgatca tggccacctt aatcatcagg caacaacttc tgtatgcgca
     9361 aaaccgccaa ccgggctatg accgggatag gttagtcgtt catttttttg aaggagatat
     9421 ggaaaaaaat tatcctctga ttaaaaatga attattacaa tctggtctgg ccgtgtcggt
     9481 cactaaaacc agcgcgccca tcacacaagg gtggagcaat acctggggtt tgtcctggca
     9541 gggaaaagat cctgatgacc gcactcttat tgaccggttt tgtgcggatg atgcggtggt
     9601 gcggacgacg ggtatggaac tcctggttgg acgtgatttt gacctgacaa aatttccaac
     9661 cgattctacg gctgcgctga tcaacgaatc cgcggccagg gtgatgggct ttgaccggtt
     9721 tgatcagccc attggacaga tcgttaagga taacgacagg gattggcata ttgtcggagt
     9781 agtcaaagat tttattcttc actctccgtt tcagcctacg gtgcctatgt ttattgcagg
     9841 ggccaaaggt tggttcaatg taatgcatgt gcgttatact aaagaccttc ccgtttccca
     9901 agtgatggaa ggggcggaaa aaatcttcaa aaaatacaac ccggaatatc cgttcaatta
     9961 tagttttacc gatgatcaat acgaacggaa attttcggat gagcgaagag tatccgtatt
    10021 ggccggattg tttgccgggc ttaccatctt catatcctgt ctgggattgt ttggtttagt
    10081 tagttttatg gccgccagca gaaccaggga actgggcgtg cgaaaagtgc ttggagcgtc
    10141 tgctgctcat gtgattggat tgttgtccag ggaatttatg atgctggtaa tcattgcctt
    10201 tgtcattgca agcccgctgg cttattggat tatgaataaa tggcttgaaa attataccta
    10261 tcggattgat attcatgggt gggtattttt cgtttctgga attgccgttg ttttgatttc
    10321 tttcgctacc gtgagtttcc aggcattgaa agcggcggcg gctaatccaa tccattccct
    10381 gcggacagaa taaacccgcc tattgactat tgactactga ctattgacta ctgactattg
    10441 actactgact actgattact gactactatt atgttaaaaa accatctcct cctggctttc
    10501 cggaatttct ggcgaaacaa agtcttttcc ctgatcaata tttccggcct ggccattggg
    10561 atcagcgccg ccctggtctt ttatttgatt gtacaatacg aattcagttt tgagcgcttt
    10621 catccggatg gggatcggat ataccgggta gtttcgaaga tcgaatttcc cgacctgacc
    10681 attcataatt caggtgtgcc ggtgccgaca gcccgggctg cccgccggga ggtttctggc
    10741 ctggatgggc tgacccattt tatcacggtt tatgatacca aggtatccat accgatcgca
    10801 ggaaacgctt caccggtggt ctttaaaaac caaaaggaaa tcgtgtatgc ggatgaagaa
    10861 tattttaagg tatttcagta ccattggctg gcgggatcgc cgcatacggc tttaaaggat
    10921 ccgttccagg tcgtattgac cgaaagcagg gctaagacct attttgccca tttaaattat
    10981 gatgagatcc tgggtaaaac cgttgtttat gatgacagcc tgaaaatgac ggtggccggg
    11041 attgtacggg atatccggga agccaccgat ttccggttta aggaatttat ttcacgggcc
    11101 acggttgaac aaaccggatt aaaacagcat tggtcctggg aagagtgggg gagcatcaat
    11161 tccggctccc agttgtttgt aaaattgtcg ccaggcacta tacccggcgt cattgagcag
    11221 caactggcgt ccattcggga taaataccga gggaataaag atcaggaacc caaagacggc
    11281 acccaacact tcctgcagcc cctccacgat atccatttca acccggaata cgatgccttt
    11341 gagaccaggc aggccaacaa atccgtatta gggggtttaa tggccgtcgc ggcttttctg
    11401 ttgctgctgg gttgcatcaa ttttatcaac ctgaccaccg cccagtccgc cctgagagcc
    11461 aaagaaatcg ggatccggaa aaccgtaggg gggcgcaagg gtcagctgat cacccagttt
    11521 ttgagcgaaa cctttttgtt taccatcctg gccgccctct tgtccctggc tataacgccc
    11581 ggcctgctga acatcttcaa ggattttatt ccaccggggg tttccatcca atcgatcagc
    11641 cggccccatg tatggctctt cctcgtattg ttggtgaccg gtgtgagttt gttgtccgga
    11701 ttttacccgg cctgggtgct tacgggtttc aaaccggtga ccgtcatgaa aaaccaggct
    11761 cattccggga cttcccaaac ccgcaaagcc tggttgcgaa aaaccctgac ggtatctcaa
    11821 tttgtcatca cccaggtgct gctcatcgcc accctggcct tcagcaaaca aattcgttat
    11881 tccctcaata aggacttggg ctatcaaaag gaggccattg tttacatcaa tacggagtgg
    11941 aacatttttt caaatgcgaa agatgaccgc cgttttattt tgcgtgaaaa attaaaacgg
    12001 atccctgaaa tcgaacgcgt cagcctgggc agttcgcctc ccgcctccaa caatacctcc
    12061 accatgacca tgaaattcaa aaatggggac aaggtcgtgg agaccatggt ggaagtgaaa
    12121 tacgccgacg cggactattt tgacctgtat aaaatgaaac tgattgcggg taagcctttg
    12181 caggaaagcg ataccacgcg ccaatttgtg atcaatgaaa cctatgcccg tatgctcggt
    12241 tttgacaagc cggaagaggc cataggccat tttatcgatc gaaatcatcc gacccccgtc
    12301 gtcggggtgg tcgcggactt tcataccaaa tccacgcatg cgcccattaa accactggct
    12361 tattcttccg agatgaacag cagttatacc ttgcatatgg cattaaaacc cggcgggaaa
    12421 gaggccggtt cctggaagaa ggccctggat gaaacggaaa aaatatacag ggaactctac
    12481 ccggaagacg actacaagta cacgtttttt gatgaaagca tcgcaaggtt ctatacctcg
    12541 gagcaaaaca tcatccggct gttgaaatgg gcggcgggct tgtgcgtctt catcagttgc
    12601 ctgggtttgc tgggcctggt catatacatc accaaccaga gaactaaaga gatcggcgtt
    12661 cgaaaggtgc tgggagcatc ggtggttcag atactagcat tgctgtccaa agattttgtg
    12721 gtgctggtgg tgctcgcttt ttttatctcc gctcccctgg cctggtgggg cgtgcaccga
    12781 tggctggaaa actttgcctt cagggtcgag atcggttggc tgatcttctt tgtggttggt
    12841 ctttccgccg ttctactcgc gctgttcacg atcagttttc agtcggtcag agcggcgatg
    12901 gccaatccgg tggaaagtct gagaacggaa tagttgaggg taaaaggtta aaggtcaaag
    12961 gtcaaaggag aatatgccgt aaaggagatt caaaggtcaa aggttaacgg tcaaaggaca
    13021 aaggagaata agcagtagag gggattcaaa ggtcaacggt taacggtcaa aggtcaaagg
    13081 tcaaaggaga ataaaggacg gcttcactgc tttattaatt gtaacctttt tcaacagatt
    13141 cagtatttaa ttcaggaacc cggcccaatt cagaccggtt ggagttatga tttgttgttc
    13201 accaatgcaa ctacaccgtt agttttatgt ggtccaatta tcttaaagtc gccctccgga
    13261 acctgtggaa acaccggagc ttttcagcca tcaatatagc tggtttggct attggcatgg
    13321 ccgcctgcct gctgatcctg cagtatgtga gtttcaaact gagcttcgat cagtttaatg
    13381 tccatttgaa ggacatttac cgggtggtca atgaccggta tcaaaacggc aaactcatcc
    13441 agcatggcac cattacttat tcaggcgtcg gcaaagcaat gaatgacgat tatgaggaag
    13501 tgatccggaa tgcaagggtt gttccatccg gggaaaccat tatttcctat ggggataaaa
    13561 aactggctga ggatcaggtg ttatatgtgg atgaggcatt tttctccgtg ttttcttatc
    13621 ctttactcgc aggggatgca tcgaccacct ttcaagagcc ttatgccgtg gtcttatcag
    13681 agtcactggc gggaaaatta ttcaattata aaggcaatga attccagcag tttatcggga
    13741 aacaaatctc cctccttccg gataccatgc tctaccaggt tgaagccatc tgcaaagatg
    13801 tacctgagca ttcacattta aactttgagc tcctcatttc atatccgacc ctcatcagta
    13861 acggctggga ggaagccggg tataatttta cccagtcgga tttctggcat tatgtgcagc
    13921 tgacgcccgg tacggattac cttaaactga atgaaaaaat gcctgccttc agtcaaaccc
    13981 attttcaggg caataaggta tctggcagcg atgaaaaatt ttatttgcag cctctgggaa
    14041 aagcccacct gtattctgat tttgaatacg aaataggccg gacagggagc gccacggtcg
    14101 tatggggcct gttactgatc gctttgttta tcatcggtat cgcctggata aattacatca
    14161 acctagcgac tgccagggcg gtcgacaggg caaaggaggt cggtgtgcgc aaagtggcgg
    14221 gcagcaccaa gggacaactg atcacccagt ttctgacaga atcccttctt gtcaatggac
    14281 tgggtatctt attggctcta ttgatcgtcc tgttggtgca aaggccattt aacacgcttc
    14341 tcgaacataa attgtcgctt gtttatcttt tttccaaagg aatgagtggt tatggcatcc
    14401 cgatcggcct gggaattctt gtgctcaccg gtgtagcggt ttcggggttt tatcccgcct
    14461 tcgttttgtc ctctttcaaa cctatatccg tcctcaaagg aaagttccag agctcccggc
    14521 aggggatcct tttcaggaaa gggctggtca ttgggcaatt cagcattacg attgccctga
    14581 tcattggctc catcatcgtg atgagacaac ttcggtatat gaaccgggcc tccccgggtt
    14641 ttgatatgga ccaggtcatg attgttcaat ctccttcgct gacttcctgg gataccgcct
    14701 ttatttccag ggtgaataac ttcaaacagg agctcaaaag gctcgttcag gtaaaagggg
    14761 ccaccacgtc ttggaattta ccgggcgggg acatcggccg gagttttaac gtccgtaggg
    14821 cggattcttc cgggacggac cgctatacca tgaggcatac ctccgtggat tttgacttcg
    14881 tgcccatgtt tagcatcccg gtcctggccg gccgaagttt cagaaccgaa gaccatcatt
    14941 gggacggcca gagattgcaa aatattatga tcaacgcctc tgcagcccgc atgttgggtt
    15001 ttgagtcccc cgaagcggca gttggtaaaa gcatttaccg gggccaaaaa aaatgggagg
    15061 tgattggcgt cctgggggat taccaccaaa aatccctgcg gtttccgctg gagcccatgt
    15121 tgttcctgcc ctattacagt accaacagcg acatcgccgt caagttaaat ccttcgggca
    15181 atttctccca aaccatttcc ttgatccggg agaaatacga agcgtttttc ccgggcaatc
    15241 tttttgacta cactttcctg gacgaagtat tcaaccgtca ataccagagc gatcgtttgt
    15301 ttggacgggt gtttgggatt tttgcgggcc tcgccatcgg aatagcctgc ctgggtttat
    15361 tgggcctgac catgttttcc acggtgcaga gaaccaggga aatcggggtg agaaaggtgc
    15421 ttggagcatc ggtcgcctcc atattgatcc tgatttccag ggattttata aaattggtgc
    15481 tcatcgctgc tttcctggca ttccccatcg cagggtgggt gatgaaggaa tggctggatg
    15541 atttcgcttt ccggataaat atggggtggg aggtatttat tatcgccgga tcaggcgccc
    15601 tgctgatcgc tttattcacc atcagttacc aggcaatcaa agcggcaagg gccaatccgg
    15661 tcaaggcgct gcggacggag tgaggttaga ggattgaggt ttgaggattg aggattgagg
    15721 tttgaggttt gaggtttgag gtttggagag gtgacaccac tttctaactt tctaactagc
    15781 taacttataa actagctaac ttataaacta gctaacttat aaactagcta acttataaac
    15841 ttgccaccta aaccatttcg atccacctgg tgtacgaaaa cggacagtcc ggctgcggtt
    15901 ggatattaca aaggcttgac aatcaactat cgtaaccttt ggtataagtt gtggtatttg
    15961 taagcaggga ctcgcaagtc atatccaatc caattttcac agatcctcaa atacctgtct
    16021 tatgttctgg tcctatttta tcatcgcctg gagaaatctg aaaaatcaca aggggtactc
    16081 cctgattaat ataggcggcc tggccgtcgg gatggccgtc gccatgctga ttggattctg
    16141 gatctatgat gaactgagct acaacaaata tcaccaaaat tatgaccata taggccaggt
    16201 catgcagcac cagaccttca acggacacaa ggggacggaa tcttccatcc ctattcccct
    16261 gggcacggag ttgaaaaaca aattcgggag cgattttaaa tacctggtga tggcgagctg
    16321 gcagggagac catatcctga gctttggaga aaaacacctg acccgcgagg gcaactatat
    16381 ggatgtggac atcgcccgcc ttctgacgct ggagatgatc cagggtaccc cggatggact
    16441 taaagatccc aattccatct tcctgtcgga atccacttcc aaagccttgt ttggagatga
    16501 ggaagccatg ggccgcaaac taaaaataga caacaaactg gatgtggccg tcaccggcgt
    16561 ctataaggat ctgcctttca aaacagagtt cagggaattg aaattcatcg cgccctggga
    16621 actgtatgtt tcatccatgg aatgggttaa gcaagcccgg aacgagaatc aatggggaaa
    16681 caattctttc cagttgttcg cccagatcgc cgatcaggcg gatatggaaa agctatccga
    16741 aaaaataaaa atggccaaac agaaccaggt ccccgaggac gagaaaaaat tcatggcgga
    16801 aatcttcctg catccgatgg ccgactggcg cctgaggtcc aactgggtgg aaggagtcaa
    16861 aaccggcggc tttatccagt acgtctggct ttttgcaggc gtgggccttt ttgtcctgct
    16921 cctggcctgc atcaatttta tgaatctgag cacggcccgc tgtgaaagga gggccaggga
    16981 agtcggaatc cgtaaatccc tgggttccga acgggcgcag ttgatccggc agtttttcag
    17041 tgaatccctc ctggtggtct tgatcgcctt cctggccgcc ctgggactga tccttggggc
    17101 gcttccgctg tttaatgaaa ttaccaataa acaaatggtc cttcctgccg gcaacctttc
    17161 tttctggctg gtcagcgccg cgtttatcgc cctcaccggc ctgttggccg gttcttatcc
    17221 ggccctgtac ctttcctctt tccagccggt aaaggtatta aaaggcactt tccgtgttgg
    17281 ccggctggct gccctccccc gaaaaatact ggtggtcctt caattcaccg tatccatcac
    17341 catgatcatc ggcacggtcc tggtctacca acaggtcctc cacaccaaaa acaggcccct
    17401 gggttatgac aataacggga tgattatgac ccagatgaaa tccattgatt tttacggcaa
    17461 gctggattta ttgaggaatg aattgataaa caacggggcc atccttgaaa tgtccgaatc
    17521 ctccagtccg ctgaccggga tctggtccaa taacgggggc ttcaactggg agggaaaaga
    17581 tcccgggctc caggcggaat ttgccactgt ctgggtcacc cccgaattcg gttcttctat
    17641 tggctggaaa atcagggagg gacgcgattt ttccagggac ttttccaccg acagccaggc
    17701 catggtcatc aatacggccg cggtccggtt tatgaatatc gaccaacccc tgggcaaggt
    17761 gatccgctgg ggagatgatc agaatgccag gacctatacc atcatagggg tcattgacga
    17821 tatgctgatg caatcccctt atgaacctgt aaaacaaacc atttatttta tgaaccctga
    17881 gcgggccaac tggatgaatt tcaaactaaa cccggccaaa agtgtggacg aatccattgg
    17941 aaagatcgaa gcggcattca aaaaatacat tccatcggct ccttttgatt acacctttgt
    18001 ggatgaggag tttggtaaaa aatttgaaac cgaagagcgc gtcgcccgcc tggccgccct
    18061 gtttgccatc ctggccgtat ttatttcctg cctgggattg ttcgggcttt cttcctttgt
    18121 ggctgaacaa aaaaccaagg aaatcggggt gcggaaagtg ctgggcgctt ccgtggccaa
    18181 cctctgggct ttgatgtcca gggattttac ccggctggtg atcttatcct gtatcatctc
    18241 cattcccttg tcctattatg tgctcaacgg ctggctgctc aattatacct acaggacttc
    18301 attctcgtgg tggatcttcg ccatcggagg ggccggagcc cttttcattg ccctggtcac
    18361 ggtgagtttt caagctatca aagcggccct ggccaatccg gtaaaatcat tgaggaccga
    18421 ataataaacg ggaattcatg ttcagcaatt atttcaaaac cgcctggagg aacctcttaa
    18481 aatacaaaac ctactcgggc atcaatatcc tgggcctggc caccggcctg gccgccttca
    18541 ttctgatctc cacctacgta agacaggaat tgagttttga ccggttcaat gcgaaagcgg
    18601 accggattta ccggttgaac acggaaatca aattcgggga caatcatctg gacctggccg
    18661 tctgcagtcc gctggtcggt gaaaccattc tgcgggagct cccttcggtg gaacaagttg
    18721 cccgccttag atggtatggg gaattcctgg tgaaaaagga agatgaaaat gtcaaagaaa
    18781 cgaacgtggc ctgggcggat gcctctcttt ttgaagtctt cagcctgccc atgatcgccg
    18841 gggatcccga aaccgcctta aaggatccag gttccatcgt cctcaccgaa acggttgcca
    18901 ggaaatattt caacagcacc gatatcattg gaaaaatcct gacggtcaac aacagggaac
    18961 aaaagaaaat aaccggggtg atccgggacc tccccaacac ctgccatttt caatttacca
    19021 gttttctacc taatgcggat gatccttcct cgcgagaaga tacctgggcc ggcagtcaaa
    19081 actggaacac ctatgtattg ctcaaaccgg ggactgccct tgaccagttt gccaacgaca
    19141 tcaaccgggt ctatgataaa ttcctggaac cccagctcca aaaagtcatc aacaagtccc
    19201 tctcagaatt caacgcctcg ggggactttt tcaaactgac tttaaccaaa ctgaccgaca
    19261 ttcaccttcg gtcttccagg gccggtgaat tatatggtag cggaagtctg cagaacgtat
    19321 atatcttttc cgcgatcgca ctttttatcc tcctgatcgc cagcatcaat tttatgaatt
    19381 tatcgacagc ccggtcctcc aaccgggcca gggaagtagg tgtccgcaaa gttttgggtt
    19441 cctccaggcc ggcactgatc tctcagttta tttccgaatc tttcctcacg gttgcactgg
    19501 ctttaatcct ggccgtgtgc atcggatact tggccttgcc ttattttaat accctggtcg
    19561 gagcgggcat tgaccgggaa tccttaattt cagcagggat ggtcttgccg ggactggcgc
    19621 tcatccttgg ggttggcctg ttggcaggca gttatcccgc tttttatctt tcttcattta
    19681 tcccggtcaa agttttgaaa agcctgaaag ggacggctat gaaccgctcc ctgctcagga
    19741 acgccctggt tgttttccaa ttctcaactt ccgtcgtcct catcgcgggt accctgatcg
    19801 tctaccgcca gatggatttt atccgcaaga aggacatcgg ctacaaccgc gaccagatgc
    19861 tgatcatcaa caacacttcg cacctgggca ataaaatcga tgctttcagg ttggacctgc
    19921 tccggctgaa tggggtggat caggcggctg tttcgggatt cctgcccgtc aactatcaca
    19981 ggaccagcga ttcctatttt cccgatgctt ccctgtccac gcgggacgcc atcagcatgc
    20041 aaagctgggg gattgacgag tattatattc ccgccctgga gatgaaaatt ttaaaaggac
    20101 gcaatttttc accagacagg aagtccgaca gcctgggatt gattttgaac gaatcggctg
    20161 cccggttctt tggagcagga gaggtcattg gtaagaaatt gtaccaggtg aaggatgaat
    20221 ccaccaaaga actggccatc tacgagatca tcggcgtggt caaagatttt aattacagca
    20281 cgttcaggga ccaggtccaa cccctgtgtt ttacatttga gaaagacccc gggagcctgt
    20341 ccctccggat gcagacggcc aatattccag acctcctggc ggagatcaaa accctctgga
    20401 aatcctattt gccttccctc ccttttgaat attattttat ggatgatgaa tacaaccgaa
    20461 ggctggaagg ggaacagaag accggggaat tattttcctt ttttgcaatg ctggccatct
    20521 tcattgcctg tctgggtttg ttcggactgt ctgcctatat ggccgaacaa agggccaaag
    20581 agattgggat ccgaaaggta ttgggagcgt cggtcagcgg catcacccaa atgctttccc
    20641 gggacttcct gaaactgatc ttgatctcca ttgtgctcgc agtgccagtg gcctattttc
    20701 ttatgaaccg ctggttgcag gaattttcat accgggtcgg tatacaggcc tgggtgtttt
    20761 tacttaccgc cgggctgatc ctgctgattg ccgtattaac ggtgagtttt caggccgtca
    20821 aagcggccct ggccaacccg gtaaaatcat tgaggaccga atgattgaga agcgagatgc
    20881 cagaagcgag atgccagaag cgagatacga gatacgagat gctgaccaac tgataaactg
    20941 ataaactgac cttatgggtg aacggtaaag ggttaagggt taaaggacca aaattaccaa
    21001 tacgagatac gagaagcgag attcgagatt gattactgac cactgactac tgaccactaa
    21061 tcaactgata aactgataaa ctgataaact gattaatgac ctcatgctaa acaactacct
    21121 catcctcgcc tggcgcaacc tgatgaagag aaagggcttc agcctgatca acagtttggg
    21181 tctggcagca ggcattgcca tttgcatgct cattgtcctc tttatcaggg acgaattgag
    21241 tttcgaccgg tttcacccca acggggaccg ggtttaccgc atggtggtgg accggaaata
    21301 tcccggacga tccacctcct actccatgat cccccaatcg tattcgcagg ccgtaaaaca
    21361 ggaatgcccg gaagtggaag cgaccacaag ggtgtttgat ttcctgggag gaagcgcggc
    21421 gcaataccgg tacggggaga aaaaatttga ggaattccgg gtccttcagg tggattccac
    21481 tttttttgaa gtctttgcag gagaatttgt ttcaggcgat ccggccacag ccctcgacca
    21541 accgaacagc gtggtcatga atgagtccac cgctgcccgg tatttcggtt cggcggatga
    21601 ggcgcttggg aaaaacattc agccggaagg cgacaataac cagccgctca gggtcaccgg
    21661 ggtatgcaaa gactggccct cgcattccca cttcgatttt gacctcttgc gcaccacggc
    21721 aggcaatgcg tttgctgcgc gcgaaaactt tgtgggtttc gcggcccata cttatttttt
    21781 attaaagcta aatacttctc cgggacaggt ggagcaaaaa atgcccgata tcatcgggaa
    21841 atatgccgca ggggatattg ccaggcaatt ttcagtaccg tttgaacagt tccaggcggc
    21901 aggtaacgga taccggtatt atttgcaggc tctcacggac atacacctgg attcaaggct
    21961 cgaaggggaa ctgaagccca atggaagcag ggtagcggtc tatgcttttg gcatcatcgc
    22021 cctgttcatc ctcgtcctgg ccatcatcaa ttttgtaaat ctgtccaccg cccgttccgg
    22081 tgaaagggct agggaagtgg gtatccggaa gactttcggt tccggcaaga aaagcctgat
    22141 gttccagttt ctgagtgaag ccgtgctgat ggggctggcc agccttatta ttgccgccgg
    22201 cctggtttac ctgatcctgc ccttttttaa tcaattgtcg ggaaaggcct ttacgatgaa
    22261 taacctgctg acggggccca atatcctcct gttttccggc ctggccctgg gcaccggctt
    22321 gctcgccgga tgttatcccg cttttgtact ggcttcgttc cggccggttc aggtgcttaa
    22381 gggcaaactc caatccagcc aatacggcct gttcctccgg aacggcctgg ttgttttcca
    22441 gttttcgatt tccatggtgc tcatcatctg caccctaatg gtcaaccgcc agatgaatta
    22501 catgaccagc aacagccttg gatacaacaa ggattatacg gtccttatcc agcgaaccga
    22561 cctgcttggg ccgcaaaccc aggctttcaa aaacgccctc ctgcaggtac ccggtgtgaa
    22621 aaacgtaagc ggagcttccg cctttccggg agaccccaat tatttcggca tatcctggag
    22681 gcaggtggga agcaatgaac ccatgaccgg ccgggggatc ctgacggatg atcaatacca
    22741 gtccctcttt gaactcgaac tcgtggaagg gcgctttttt tcgaaagatt acggtaccga
    22801 ttctcttgcg gtggtcttga atgaggccgc ggtaaaggaa cttggcctgg ttaatcccat
    22861 cggagcccgc ctgatttcag atgaagattt tttaaacggg cccgacaacc aatcttatga
    22921 atatcatgtg atcggcatac tgaaagatta caattaccag aacctgcatt tgccgatcgt
    22981 ccccctggtc tttaccagca catcgcggtt ccaggatgtt acgttcagcg ccgcggtaaa
    23041 actgcacggc caggaaatgc aagcggcttt gcggtctatt gaaaacgaat ggaaaaaatt
    23101 tgtaaaggac cggccttttc attacgaatt cctggatcag agcattgccc gccaatatga
    23161 ggcggaaacc cggacgcgca gcattttcag tttttttgca ggcctggcca tttttatcgc
    23221 atgtatgggc ctgctgggcc tctcggccta tgcgacccag caaagaatga aagaaatcag
    23281 catgcgcaag gtgctgggcg cctcaaccct gggcattgta agcctattga ccaaagactt
    23341 catgaaactc atcggcctgg ccatactcct tgcttttccc ttggcatggt acggcatgag
    23401 tcgctggctc cagggattca gttaccgggc agaactttcc tggtggattt ttgggctggc
    23461 tgccgcgggc accctctgta ttgccctggt cacgctcggg gcgcaggctt accgggcggg
    23521 gcgcctcaac ctgatcaggg ttttgagaac ggagtagtga ggtcggggag tggatggtgt
    23581 gtggtggatg gtgtgtagtg gatggtggtt ggaatgggga cctcaaaaac aacaatcgca
    23641 tttttaattt ttggtttgga attcgcattc ctattgattc aggaggaaag taatcccgac
    23701 tttccgaggc ccaagtctca gatagaagac cggatcgggg ggagactttt ggaagggact
    23761 tctccaaacg gaaggccttt ctgatgggac tgcaaaaagt aatttgtagt ggattggttc
    23821 attctaaatc aaagggcagg caaatccttc agtccgggta taattagcag cccatgtcac
    23881 tcaacaaaag atggaagaaa tcggagtcag gtaggcgccc ggtgcaacct gcggtagaat
    23941 tgtgactccc ttatcttaag aatttgtgaa attgatctta ttagccaatc agaacgcctt
    24001 tccgattgcc aggtatggca tgcaagtatg gatccaggat gattctcttt ttgactttaa
    24061 tggtacagcc ccaatagccg ggagaatcaa tccgttgaca cgtccaattc cgcccgattc
    24121 agggctcctt cttaacctta accttaacct taaccttatc cttaacctta accttaaccc
    24181 gacgtatgtt caaaaattat ctcgtagtca cctggaggat attgattaaa aataaaatgt
    24241 attcagcgat caaccttgca ggcctggcaa taggatttgc ttgttgtgtt gcgctcggga
    24301 tgtatattta tgatgagtgg agttacgatc gttttcattt ccacaaagac aatatttaca
    24361 gagtcgtcgc caatatcaaa caaaccggcg gaactttcga tatggcggtc acccccggac
    24421 ccctagctcc agctttaaaa gcggattttc ctgaggttat tcaaacctgt cgcattgcga
    24481 gacagcggaa tgcggtaatg gaaataaggg accaggtatt tgaacccgca caaacgctga
    24541 gtgtggatca tagttttttt gaacttttcg attttaaatt gctcaaaggc aataaagcaa
    24601 ccgtattgct cgaacccgat gaggcaataa tcagtgaaaa attggccatc caatgttttg
    24661 gaaaggattg gtatttacag gatgcccttt taggaagcac gtttaaattt aatgaccatc
    24721 ggctcctgac cattgcgggc attatccagg atcctcctga tcaatcccac cttcaatttg
    24781 acctgttgtt gtcctttaaa aatgaagaaa tcagcaacgg ggggtataat tgggatagca
    24841 acaattatca tacttacgtg ttgctgggac ctcaggcgga cctcgcccgg ctgcaagata
    24901 aattattggg ttttcttgag agtaagaata agtacctgga agggaatacc ctttcgcttc
    24961 agcctttgct ggatattcac cttcgttcag attttgcctt tcaaacggat tgggctaaaa
    25021 caagcaaccg ggtctatata cgtatttttt ttgctgttgg attgattgta ttattcattg
    25081 cattgttcaa ttttattaat ttatccactg ccaaagccgt tgaaagaatg aaggaggtcg
    25141 gggtgcggaa agcgattgga gccgcgagag gtcagctgat aacccaattt tttgtcgaat
    25201 cgttgacgtt gtctttcctg gccattttta tttcttatgc cttgttactg ggttttatac
    25261 ccatattaaa ttccgttgcc gacaaatctt taagtattcc ttataccttg aattttttat
    25321 tggtcttgac tggattttca tttgtgtcgg gtctattggc cggcatttat ccggcgatct
    25381 atttatcggg attcaaaccg gtaaaggttc ttaaagggat ttttgatttg aattcaagca
    25441 ggttcaggca ggtactggtc atcagtcaat ttaccccgtc gattattctg attatcgtaa
    25501 ccatagtcat tcaccatcaa attaattatt tgcaaaacaa agacctcgga tttgaccaat
    25561 cccaattgtt gagccttaaa gttaaaacca aagaaattgc cccggtcaac ttaatgaagc
    25621 aggaaatgat tgcgcaaccc ggtattgctt ccgttgcggc cacttcttct accctgatcg
    25681 atgtgatcaa ttccacgggg agggtcaaat ggcagggtca gggggccgat gaaaaattaa
    25741 tgtttaccac cataaatatt gatccggatt acctgaccac ctccggaatg cacttaattg
    25801 ccggcaggaa tttcgatcct ggcagaccag tggataacac caaatcattt atcatcaatg
    25861 aaactgcagc ctctaaattg ggttggaccc cggaggaagc agtaggcaaa tcctttacga
    25921 tctgggactc tacaggcaac atcattggag taaccaggga ctttcattat aagccgctta
    25981 cctcgtccat tggagcattt gtattcaggt actggcctgc tgaatccttc cagtacctcc
    26041 tgataaaaat taaacccaat catatccggg aagccataag caatatcgaa aaggtctata
    26101 aaaaattcga tcctaaaaca aatattcaat atgaatttgt aaatgaattg gtggagcatc
    26161 agtaccggac tcagctgcgg tcgggaaaga tcattttata tttttccatc ctgaccatct
    26221 tcgtttcctg cttggggctg tttggactgg tcacctttac caccgtccag aagaccaggc
    26281 aaatcggcat ccggaaagtc cttggagcca gggtagtcag ccttgttcgt ttgttgtctt
    26341 tggattttat aaaactggtc gggatcgcta tggtcctggc agttccggct tcgatctggc
    26401 tcgcaaaccg gtggctgcaa gacttcgctt acaagataga gttgaagtgg tggatgatcg
    26461 gcggggcggg cttgttggct ctcgcctttt ctgttttgac ggtgtccctg cagaccctga
    26521 aggcggctat ggcaaacccc gtgaaaagtt tgagatcaga atagaaaagg ctgcatggga
    26581 gaggatggta aagcgtaacg tttccgaaat aatcacttaa aaataacatg ctcaaaaact
    26641 atatcctcat cgcctggcgc aacctgatca aacacagctc ctatactgcc attcatatcc
    26701 ttggcctggg tattggtttt gtggcggcct ttttaatcgt tttgtttatc cgggatgagt
    26761 tgagctacga ccgatttcac acgaatatcg acgatttgta ccggctcaat ttttacggta
    26821 agatgggcga tcaggaagct cataccagtc aggcgcccgg accggcggga cccctgttcc
    26881 agtccctgtt tcccgaagtg gaagctgcat gtcgcataaa gactgccggg gacttctccg
    26941 tccgggtggg agataaagcc tatcgggaga gcaggctggc ctatgcagac agcagtcttt
    27001 tccggctttt tacttttgcg gtccgggagg gatcgacctc cggagcacta aataggccgg
    27061 ggcaggtaat catcaatgca accacggcca aaaaatattt tggagcggaa aacgctctgg
    27121 gtaaaacact ggtactggat aaaatcaaaa cctataccgt gtcagcagtg atagaggatt
    27181 tgcctggcaa cagtcatttt caatttgatt ttttcctgcc gatggctgaa aatcaggaca
    27241 gctatgtgga aaactggggc agcaccaatt accatactta tttcctcttg aagccgggta
    27301 cccgggtaga ggctttgtct tcaaaaatga acgaatcgtt ccgggagaaa ttcaacctgg
    27361 tattaaaaca atatttaaac tcttccctgg aggagtttac cgcccagggc aactatgccc
    27421 gggtagaact ttttcctgtc cggaaaatcc acctgtatag taatctggat gatgaattgg
    27481 cgcataacgg caatatttcc ctgatttata ttttcggcct tgccggattt ttgatcctgc
    27541 tgctggcggg tatcaatttt gtaaacctgt ccacagccag ggcttcggtc cgatcccgtg
    27601 aagtgggggt ccgtaaatcg attggggctc tgaaccggga ccttaccctg cagttccttg
    27661 ccgagagtat gctggtctgc ctcctgtgct ggctcatggc catgatcctg gtttcggttt
    27721 cattgccctg gtttaacaac ctagctggaa aaagtctgga aatcggcgga ctctttaacg
    27781 cagaagtttt aattcctttg gtgatcattg ccctggtcac cggatttacc gcagggatct
    27841 atccggcatt attcctttct gcgctggaac ccgtcaaagt actcaaaggc acattgggcc
    27901 tggggctccg gaaaagcaag ttgaggagcg gcctggtcat attccagttt tttgtttcaa
    27961 ttgtactgat catcggatcc ctgagtattt accggcaggt agaatatatc cagaataaaa
    28021 aactgggtta taccaaggaa caggtgctgg tgatcagcga tgcctacctg cttggaaacc
    28081 agttggaagc ctttaaaaac aggctcaagg ctatgcctcc ggtaaaaaac ctttccgtcg
    28141 gcgccttcct tcctgtttcc tcagaacgca atacaacttc catcgtcctt ggcaacgtgc
    28201 cttcgccggg caataccttc ctggtaaaca actggtggac ggatcccgat ttcttaaaaa
    28261 ccctggaaat agaattggta cagggcaggg actttttcag ggcgtttcct gctgacagcc
    28321 aggccgtgat catcaatgaa accctggcgc gtttttttgg ttatcccggt gaaaacctgg
    28381 agggccaggt cattggcctt ccccgggata gcgggaaaat tgtaccctat catttgatcg
    28441 gggtggtccg tgattttaat tttttatccc tgcatcatcc catcgaaccc cttgcgatat
    28501 tttatggagg tactccggat tttatcacca tccgggctga aaccggtcag atccaggagc
    28561 ttctcaggga aatcgaatcc ggatggaacc aactggctcg gggcagtcct tttacctacg
    28621 actttttgga tgaccggtac cggcagttgt atggtgctga aacgaagatg ggtaaaatcg
    28681 cttttgtatt tgctttcctg gccatcttta tcgcctccat gggcctgctg ggactggttg
    28741 ccttcaccat ccaacaacga tttaaggaaa tcggcatccg gaaagtcctt ggggccagcc
    28801 tcccggattt gctttcactg gtttcaaaag actttatcct cctgatcggc gcagcgatat
    28861 tatttgcctg ccccctttcc tggtggctgg ttcaacaatg gctgaaacga tttgtcttca
    28921 ggatggatcc cggtccgggt ttattcattg gcactgcgtt gggtacgatc ctgatagccc
    28981 tggtgattgt aggattgcag acgttgaagg ctgccagggt gaacccggtg aaaagcctta
    29041 gaacggagta aaggagaaag gagaaaggtg ggacttgcag atattccaaa ttttaaaaat
    29101 ttggaatatc taagctttaa cccaaaccat gcaatagatt ttctattaca aaccggaatg
    29161 cctgcaaata ctg
//
LOCUS       DAOQXT010000056        29153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1879_length_29153_cov_7.231803, whole genome shotgun sequence.
ACCESSION   DAOQXT010000056 DAOQXT010000000
VERSION     DAOQXT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 29153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29153
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1879_length_29153_cov_7.231803"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1764)
                     /locus_tag="PKM27_10880"
     CDS             complement(112..1764)
                     /locus_tag="PKM27_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07808.1"
                     /translation="MKKLIYIGILLSMGIIPNVLAQKIGNLNGIYYQAVAVDETGKEI
                     VGTDISGKPLYNKAIGVRFAITKGLNGPVQWEETHTTTTDKFGLFTLIIGKGTSTGAA
                     PYPRMLDIPWIEADQYLKVELAIKNDGNYKLVSNQQFMAVPYSFYTDDIAEDAITTLK
                     ILDSTILNQDLRTGSVDTRTLLDSTILNRDLRTGSVDSRTILDSTITSQDFRTGAVDS
                     RIILDSTILNQDLSTGSVDTRTLLDSSVINQDLSAGSVDSRSILDFSIANQDLLTGSV
                     DSRIIQDSTLANQDLRTSSVDSRTLLDSTILNQDLSTGSIDSRSILDSSIANQDLLTG
                     SVDSRIVRDSSLINQDFNTGAIDSRLILDGSLKNEDLADSTIDLASKVTGILPVMYGG
                     TGADSLAANSLLLGNDRGGIHALGKATDGQIPVGITDSLPKLKTLAGKDGVKVTITAD
                     SVLISSGILSPGIKSIQSIVADTINPGIISIFTTWLSPPIAVEGATMESVVIGSIPVD
                     LNGCQMTTFVSEPGIIKVAITNLTSNFVDLGTNRNLKVVIMN"
     gene            complement(1789..2421)
                     /locus_tag="PKM27_10885"
     CDS             complement(1789..2421)
                     /locus_tag="PKM27_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNR07809.1"
                     /translation="MKILKSIYVLCLLAACSLTGLAQSGLVLEASQLFSSFKFKDRAG
                     VSHNKEYQGLYTGGYGIGYRYVNDIGLIIKPGIGVRHGGANLVYDDQNYSWRLQYAEI
                     KLGLGYMTRFEKINPYFMASGYYAFLLRGAQTTHNENLNITESDLLSNIDYGLIFSPG
                     VNIKLSDYISSYLELNYFKGLTNIEKDPGQKTKNFAYGLSLGVAISLTEN"
     gene            complement(2429..2938)
                     /locus_tag="PKM27_10890"
     CDS             complement(2429..2938)
                     /locus_tag="PKM27_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07810.1"
                     /translation="MKKKPILFVAGPGIVLLFLLSGPVHAQSIKRQSIPAYGSSTQTE
                     TVSISQTAGQAYNTTVHPNEITVSPGFQQGPRYHVVQIPAPVLNTLNITVYPNPASHS
                     VTLASDAEIEQAIISVTDVNGRFLHSEKVMKLTSHPLRCASWANGVYMITIQDPGRRV
                     KTIRLIISK"
     gene            3408..5648
                     /gene="katG"
                     /locus_tag="PKM27_10895"
     CDS             3408..5648
                     /gene="katG"
                     /locus_tag="PKM27_10895"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNR07811.1"
                     /translation="MEKNTSEALASPKQNGQSKCPFHGGALKQTAGGGTRNRDWWPNQ
                     LKLNILRQHSALSNPMGEKFNYAEEFKTLDLNALKKDIFELMTNSQDWWPADYGHYGP
                     FFIRMAWHSAGTYRISDGRGGGGSGSQRFAPLNSWPDNVNLDKARLLLWPIKQKYGRK
                     ISWADLMILTGNCALESMGLKTFGFGGGREDIWEPEEDIYWGSEGKWLDDKRYSGDRE
                     LENPLAAVQMGLIYVNPEGPNGNPDPLAAARDIRETFGRMAMNDEETVALIAGGHTFG
                     KTHGAADPSKYVGPEPAAAGIEEQGLGWKNSFGSGKGIHTIGSGLEGAWTTTPTKWSN
                     NFFWNLFGYEWELTKSPAGAHQWKPKHGMGANTVPDAHDPALRHAPTMLTTDLALRVD
                     PAYEKISRHFMDHPDEFADAFARAWYKLTHRDMGPISRYLGPEVPKEVLIWQDPVPAV
                     THKLVDEQDIEALKKKIKDSGLTISQMVTTAWASASTFRGSDKRGGANGARIRLAPQK
                     DWEVNNPSQLANVLKTLEGIQSEFNKSQSGGKQVSLADLIVLAGCAGIEKAAGKAGHS
                     VTVPFKPGRTDASQEQTDVESFAVLEPAADGFRNYLKPGQVASAEEMLVDKAQLLNLT
                     APEMTVLVGGMRVLNANFDQSKHGVFTKHPEALTNDFFVNLLDLNTGWNAISEAKDVF
                     EGRDRKTGEVKWTGTRVDLIFGSNSELRALSEVYGSSDAKEKFMQDFVAAWTKVMNLD
                     RFDLAD"
     gene            5897..9151
                     /locus_tag="PKM27_10900"
     CDS             5897..9151
                     /locus_tag="PKM27_10900"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNR07812.1"
                     /translation="MNKTIFILLVFVYSFSAGQDSSGKLDSEFRNPPKDTRPWVFWYW
                     VDENISKPGITKDLEAMARVGIGQALIGHISPGPVRGDARMLSKEWWDMVAFAVKEGQ
                     RLGVDIGFFNGPGWSQSGGPWISLDQSMRHVVSDEWRVRGPALLREKLHVPGPGFKQI
                     ALQAFPVDSRSAKSPGPSDMISFQARPETGNIYKLFDRDTATSYSFPAGSGSNEKITL
                     DLEFKEPWTIQAIKFDYPPVSFYTQVDFQSAEPDGMYRSIRKFKIDRRNIQFQIGPKR
                     FEPLWFAVPATRSRRFRFVFDQIRATDSLRFKEIELYTGTVVDQHIDKQLGKMASDPV
                     PLWDAYLWPEQEEPGNGDLIKPESLLDLRSRVDAEGFLNWEIPPGDWVILHSGMAPTG
                     AVNVPVPPEATGFECDKFSKEAIKTHFQAFAGQFLKTIPRAERTALKTIVIDSYEVGS
                     QNWSNDFAALFAERFGYDPLPWLPVFAGRIVKSADLSNRFLWDVRRLGADLIAENYVG
                     HLRKLSNRNGLDLWLENYGHWGFPAEFLQYGGRADMVSGEFWFENPYWDLGPIECRAA
                     SSAAHIYGKNRVFAEAFTAGFNFKQYPASMKSRGDRMFCEGINHFVLHLYIHQPWENR
                     VPGVTAWFGMSFQRNNTWFEQSKAWVDYLRRCQYLLRQGDPVSDLCYFTGEDVPKMTG
                     ALNPALPSGYDYDFINAEVLERARARDGKIILPHGKEYRLLILPEQTTMRPAMLEKLE
                     KLIADGAAVYGPPPSRSPSMAGYPGSDQEIMRLAMRIWGNKGTVTASGRSYGKGKLYR
                     DQDLAGVLEDMSLPPDVLMADTGILWTHRSSMGLDIYFLSNLDDREKKSWISFRVKNR
                     VPELWDPASGRISELSSYRMEGERTKVPVCLDPSGSVFIVFRKAGGEAAEPKARSTHA
                     VKEIALDEPWQLHFPGGWDAPDSITVKDLTSWTDFSDMGIKYFSGTARYKNTFNIPVE
                     TLKENTRVILDLGEVRMMAEVTVNGRNLGVLWKKPYKTEITEAIRTGDNQVEIRITNT
                     WWNRLVGDAKYPEGLPGSEVHAPRTFVTVKGWTGEEELMDSGLIGPVVVKIREE"
     gene            complement(9285..9695)
                     /locus_tag="PKM27_10905"
     CDS             complement(9285..9695)
                     /locus_tag="PKM27_10905"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNR07813.1"
                     /translation="MTTEQNKKIVYRVNKEFIEGGNINTVHEIFAPDFINYTAPPGSP
                     QGPDAVIYFFNQLLKPAFPELKVEIHEMIAEGDKVTTRKSFHAIHKGEFFGVKPTNKS
                     VVMEVIDIVQLRNGKYIAHWGILDMHNLMTQLAA"
     gene            complement(9782..10714)
                     /locus_tag="PKM27_10910"
     CDS             complement(9782..10714)
                     /locus_tag="PKM27_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR07814.1"
                     /translation="MYKSVSTGIRQVKLDTELYKIHKEGKGKSDFGMDNSSGLIDHGF
                     GLYSTRDVKKKIEHIKTEYFRISLTRQGSARFDIGLEKYRPVRNWILFGIPGQIFSLY
                     DVNPDFLAYYMLFTEKFISSTSLFANKTWKFPFLNHSGVQCFELKEETASEIEKIIFK
                     INDEVRNRKTHLSETIKLYIQLILIHASRDYDAVQLSSQGSSNPAQNLFFAYLKLVSR
                     HFLTVRKVAEYADLLHVSPDHLNRAVKTCSDKTARDLIDEMLLMEAKVYLLHSQSSVS
                     EIAYKLEFADPPHFNRFFKKMCRQTPLEFRNASE"
     gene            complement(10816..12246)
                     /locus_tag="PKM27_10915"
     CDS             complement(10816..12246)
                     /locus_tag="PKM27_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR07815.1"
                     /translation="MKFLNLNLNLNLNLNLNLILNLILNLILILKNLSMKTMTKTFIL
                     LKVCLLAILTLPAQTSKPVKEMAYADSPRLVEIFKDLHQNPELGFLEFRTSGIVAKEL
                     KTLGFEVITGIAKTGVVGVLKNGEGPIVMYRADMDCNSVKETTGLPYASVKTMKKEDG
                     TEVPVMHACGHDAHVTWMLGIARIMMALRDQWKGTLVFLAQPAEEPMTGAKAMVNDKM
                     YEKGVPIPDYLFGMHTWPIAVGSIINGTGERTSGSDQLDVTFYGIGGHGSTPELAIDP
                     IVMASNAVLQYQTLISRSLPAQDVGVLTVGSIQAGSDNNVIPAYAVAKLNLRWFTEKT
                     RNTLLEGIKRINEGLAVANGLPHELHPKILMKSNVFPQVNHTPLVRKINNALTPLLGP
                     ENIFSDTPPIMASEDFYHLVLGNNKTTCDFILVGTADPELYAQAVREGKRAPFFNHNG
                     NYQVDLAGIPLGVQIGVTALLEIFRK"
     gene            complement(12243..13097)
                     /locus_tag="PKM27_10920"
     CDS             complement(12243..13097)
                     /locus_tag="PKM27_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR07816.1"
                     /translation="MNLTFGVSTWLWTSPFTSETISLFPKIKSMGYDAVEIPVEYPEK
                     IKAKEVKAALGDHGLKAIVCGAFGPTRDLTHEDPAVHENCFAYIRECLDLCTAWDTGF
                     LAGPMYSAVGKARMVPPEQRRAEWDLAVRNLHKVCRMAQERGLAIALEPLNRFESDLV
                     NTAEDVMRLVKEVSHPAAKVMLDGFHMAIEERNLEEAITLTGDKLIHVQVSENYRGTP
                     GTGQTDWDSLKRGLKNIRYQGALSIESFTPEIKELAGAVCIWKNLAENQDFFAQEGLR
                     FLKNHFKD"
     gene            complement(13216..14424)
                     /locus_tag="PKM27_10925"
     CDS             complement(13216..14424)
                     /locus_tag="PKM27_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR07817.1"
                     /translation="MNRLFLFFFSITALPSFTQSKPDRIVHNDPAQYRVLRAVHAGAG
                     QMQFAQLIGRDVLSTNFLYLHAGVIESKGGIGHHFHHQIEEMYVLLDGEAEFTINGRT
                     SRLQAPVAVPCKQGDAHAIYNPTDGPVRWLNFAVSKFKGQGDAFDLGDNRVGASIDRV
                     PVFVSGSLNKEGLKDNQHPYAGQGALCKRAFGPEIFSTHWNHVDHLVIPAGKSVGPRR
                     LEGIEEVYYVIKGSGTLTVNSSTQPIVADDSFTGSLGETLALSNSGTADLELLVIGVA
                     ASQNKEANPAKPKAMVLQMDFVVPEENSEAFEKMYHSIYVPAMTVQQGYLGSRLVRLF
                     PKEVAKSIQAEPTTFNYQIQISFDTEENRRKWVASDQHQIAWPAASRLAKEYKWRGYE
                     VKGEDHPGFR"
     gene            complement(14516..16759)
                     /locus_tag="PKM27_10930"
     CDS             complement(14516..16759)
                     /locus_tag="PKM27_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNR07818.1"
                     /translation="MFKPVSHIRYALLITAFLIFYAGCQPGPASTGHDTWQVYGGDPD
                     QSRFFNGSQITRKNVDQLEVKWVYPASDSQAYFFSPIIVDTFMYVLAKNFSLIAVHAL
                     TGKEIWIHSRLQGISRRGINYWESEDKRDKRLLFTLNNTLQAIDATTGKSILSFGNNG
                     YVDLREGLDRDPSSITRMQAMMPGALFEDLILLGSAPGENYFSPPGYVRAYHVVTGKL
                     QWTFHTIPRPGEYGYDTWPKDAFKYVGAVNVWSEISIDKKRGIAFLPLGSPTYDYYGA
                     DREGSNLFGNCLVALDARTGRRLWHFQTVHHDLWDYDLASAPQLITVIREGNKKDAVA
                     VATKHGFLFIFDRVTGEPVFPIEEKPFPASAMPGEKAWPTQPIPVMVPDFTRHAVTKE
                     TLNPYLSAEVKEQWMKRLDSARSGLFIPPSDQYETVMMPGALGGANFGNTASDPEKGM
                     MYILTQEHASIYKLNKVLPPQASLSALEINKLNRLYINSCQTCHGKNKEGGLGPALVN
                     AGQRILYDEFKKVMENGKGQMPGFVHLDEQTLAALYRYLGGIPNRMNFGRRPDAGGEM
                     EGPVVASGGAAITPDEKRSAPMRDYPEGVPHPEIRYTTDYGLDWPGLLGPPWSSLLAY
                     DLNTGTIKWRKPVGEDSTFTKGDKNTGAPGGTLRKGMVVTSTGLVFATAKGGKLYAFN
                     GDRGDLLWETTLSYESNAQPSMFERNGRQYLVINATGPFAPDSYNHSLKPGALPRGYV
                     VYGLREK"
     gene            complement(16812..20303)
                     /locus_tag="PKM27_10935"
     CDS             complement(16812..20303)
                     /locus_tag="PKM27_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNR07819.1"
                     /translation="MKGLAQTCIYLLATILGIPSCTSPGASLRDGSGKLEILFLGHEK
                     EHHNSARFLPLLASSLSKEGIFFTYTNNPDDLNEKTLGLYDALMIYANHEEITPAQET
                     ALLDYVNKGGGFVPIHCASFCFQNSPKYIDLVGGQFKNHGTDTFTAAIIQNEHPVMKN
                     ISAFSTWDETYVHDKLASDIEILMERVDGDHREPWTWIKTHGQGRVFYTAYGHDERTW
                     GHPGFQQLVKEGLLWAVGDEAKLKWEKFKSEIPQLVYRDEPGIPNYEKRVPGPRFQEP
                     LSPEQSAKLIQVPVGFDLKLFASEPDIINPIAMDWDERGRLWVIETVDYPNTVREDRT
                     IGDDRIKICEDTDGDGKADKFTLFADNLNIPTSMVFIHGGVMVSQAPYFIFLKDTDGD
                     DKADERKIIMEGWGTFDTHAGPSNLKYGLDNQIWGVVGYSGFDGVIAGENRKFSQGIY
                     RFKPDLSSFEFMTGTSNNTWGLGITPNNDVFASTANNTHSVFLGIPAKTMKDVQGIRP
                     LGSSKIDGHYAMRTITDHVRQVDVFGGFTAASGHNFYTARAYPREYWDHTALVCEPTG
                     HLVHLAKIEKDGAGFIEKDGWNLFASADEWVSPVDAKTGPDGAVWVLDWYNFIVQHNP
                     TPTPERGGFAGENGKGNAYENPLRDKSHGRIWRVVSRQAKPYKPMRLNPGDPDGLIAA
                     LNHDHMLWRLTAQRLLVERGKPDILPALYRLARENKDGNGYAAIHALWTLDGLVDLSK
                     EDKAQSVVLESLGHPSPGVRKAAIQILSKAGWREETVIKNNLLNDPDPNTRMAAILSL
                     AELQPSDSLGNVLYKMSLEESVIGDEWLSKAIYVAAVRHKNGFLQAFLADHPGYDPSQ
                     VKSRNRESLTYDDESWNKMILPTFIEDAGLDIDGLIWFRKTLDIPVSMAKKAKLSLGP
                     VNDSDISYINGVRVGSTESRHLDKRLYEVAAGILKPGKNVIAIRVEDIGGRGGIYGKP
                     EELFLAAGDQKRSLAGEWKYEVAQEQGPAKTLFSDKSIGETFCEAYLGSLPGTAPDLV
                     ENSGEAILIHLKTIKNEMKYDQKTFSVEAGKPVEIVFENPDFMQHNLLITQIGSLETV
                     GKAADKLAADPQGAERQYVPDLPEVLFSTKLVNPQETVKLKFIAPEKPGDYPYVCTFP
                     GHWLIMNGIMKVKAPSL"
     gene            complement(20332..22260)
                     /locus_tag="PKM27_10940"
     CDS             complement(20332..22260)
                     /locus_tag="PKM27_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR07820.1"
                     /translation="MIKSLLSLFSILVALAPPAFAQPAEPYAPEGFDRLRENIPHGKI
                     DSVFYPSRTVGVNRKALVYTPPGYSTAKKYPVLYLLHGIGGDEYEWMRGGRPDIVLDN
                     LYAEHKIEPMIVVMPNGRAMKDDRAGGNIFDSLKVQAFATFEKDLLNDLIPFIENKYP
                     VLTDRNSRALAGLSMGGGQSLNFGLGNLDQFAWVGGFSSAPNTKRPEDLLPDPVAASK
                     QLKLLWISCGDNDGLLRVSQRTHDYLFEKGVPHIYYLEPGVHDFKVWKNGLYMFSQFL
                     FKPVDPSTFSRYTVLGFPASTNIRSAKYPQILPDHRVRFRIKAPDAQKVEINLGKRYV
                     MERDTGGYWMVTTDSLGEGFHYYSLLIDGVALADPASEAFYGMGRMASGIEIPFAGGG
                     YYAIKEVPHGELRSKRYYSPSLQTWRRFTIYTPPGYDENATERYPVLYLLHGGGEDER
                     AWAAQGKTDLILDNLLAEKKALPMLVVMLDGNIGSGGLAGFNEQILRSFENELKQSVI
                     PFVEKNYRVKTDAQNRALAGLSMGGLQTLYAGIRNFNLFSWLGVFSSGWFANRPELSN
                     PQYEFMKTNAGEINAHLKSLWVSMGGQEDIAYNNCKIMMEKFDELKVKYVYSEYPGGH
                     TWPVWRNNLYSFAQILFK"
     gene            complement(22362..22883)
                     /locus_tag="PKM27_10945"
     CDS             complement(22362..22883)
                     /locus_tag="PKM27_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR07821.1"
                     /translation="MKIAVSGAHRVGKSTLVENLLESLPEYTSRAEAYHELEEQGHHF
                     SDLPGLDEFFLQLDYSIEQIAKGGNCTLFDRCPVDILAYIQLSYALGHVQNQTLYQRV
                     FKAMSTLDLLIFVPIENPDVIACPENELSELRQRVNELLHEWVWDFDVPVLLVSGSAL
                     SRRNQVLQYLSKI"
     gene            23151..23972
                     /locus_tag="PKM27_10950"
     CDS             23151..23972
                     /locus_tag="PKM27_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR07822.1"
                     /translation="MKIFKTLKGMHTAGKAGMIFLLMGSCEKVEFDPALKHEFTIQSA
                     ADGSEYRIEVGLPAGYHPGGEKYPSLYILDGEENFDFVSSQCSEISDMLGVQNVVTIS
                     IGYGNDRSLDYTPTEVSAVTGGGPRFLHFIETQLIPAMEGYFSVDTSRGSRTIMGHSY
                     GGLFGAYAFSVNNRLFGNYILLSPSLWFDDLVSLQFEKQYRTENKDRSQLVFLGIGEA
                     ENLGRMQAPFEAFYQTLQSQYPHIKLTKNKEENLGHMGSRNPNIVKGLHYYFQNR"
     gene            24012..24626
                     /locus_tag="PKM27_10955"
     CDS             24012..24626
                     /locus_tag="PKM27_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07823.1"
                     /translation="MNSIKNICLLIAILFSMPLTAQRTFSSLSKEFNTLPKLTLEPGV
                     GIHTNFGTDFLITTLIQWNWLDRLSFASHSSFNINNITQRSFNGIKTRYNYSINQKLG
                     LGTTFYTGGNSHSVLLMAGIKYTSYRETLEDARFEPFGTAIHALSPDYGLMYSHKKIW
                     KKYFFTYRMYVPLYPWPAKGSDLLYLEGNMDNIALEVGLGIRII"
     gene            24730..25659
                     /locus_tag="PKM27_10960"
     CDS             24730..25659
                     /locus_tag="PKM27_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR07824.1"
                     /translation="MKKMVFIIPPTVELLDLAGPVQVFTEAIFYGFETEIEFYTYQDN
                     PVSTSGLGFGKISHYSEAKLKEGDYVFVPGMHEDYVRSISFKAEKAFFNWLKECSDKL
                     ITVGSICNGAFALGHAGLLDHTECTTHWRRVNELQTQFPKAKVVADILYVRSNRVYTS
                     AGISAGIDLALAILEDLKGALFTHKVARGLVVYHRRSGKHKQHSVYLDYRNHIHPQVH
                     EVQDYLLENLSKENSIGSLAVRVGMSPRNLSRVFKEKTGITVLEYLTNLRIEYAKTML
                     NNPEYTVDYIASKCGFKSARQLQRILKKQNGNK"
     gene            25656..26249
                     /locus_tag="PKM27_10965"
     CDS             25656..26249
                     /locus_tag="PKM27_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNR07825.1"
                     /translation="MNNHSDSAKIQKARTTGLFFLLAFLAYGLGRHLFKSEGGPEKYL
                     GAALIFSNSIMVLFIGLFLRETLKQSNVWVGNIYLMARVFEALALASILLNLMPSLNI
                     PDDAGYFPAMLVLGLGSIPMCLELYKQKISPAWLALGGLIGYTIFSFGFLMEFLGKKW
                     SMYLLAPGGAWEITFAVWLMIKNGPNKIHDPSPAKAG"
     gene            26480..28357
                     /gene="htpG"
                     /locus_tag="PKM27_10970"
     CDS             26480..28357
                     /gene="htpG"
                     /locus_tag="PKM27_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNR07826.1"
                     /translation="MSEKGQIAVQTENIFPIIKKFLYSDQEIFLRELISNAIDATTKL
                     QTLSSKGMAKGELGDLTIDILLDEKKKTLTIRDRGLGMSAEEARKYLNQVALSSAQEF
                     LEKYKGETNIIGHFGLGFYSAFMVADKVEVLSKSYNEEEKPILWTCDGSPEYTLKEHK
                     KKDRGTDVILHVNDDGKDYLEKNKIEELLKKYCRFLPYPIRFGTRTEYDWEGEGENRK
                     EIKKEVDNIINITQPIWKKSPTELTDEDYKKFYSDLYLFSSPPLFWIHLNIDYPFNLT
                     GILYFPKVTTGMDWQKNKIQLYSNQVYVTDEVKDIVPDFLMMLHGVIDSPDIPLNVSR
                     SYLQSDRQVKKITSYITKKVADKLEELFKNDRPSFESKWQDIGPFVKYGLITDEKFYE
                     KAIGFSLLKSEDGTYYTPEAYREKIKDQQTDKNGQVVYLYTHAPELHHASISAARARG
                     YDVLLMDQPIDNHFMQHLEMKIEKTHFTRVDSATLDKLIEKEDKIESVLSQEEQDKVR
                     TLFETFTQGTMNKVEVQALSPSDAPVHITKPEFIRRMKEMQMMQGMGSDLFPDSYQVI
                     VNGNHPLVAEKLLHATDEARQKELATHLYRLALLNQNLLKGAELTDFVSKNLEALSRN
                     D"
     gene            complement(28359..>29153)
                     /locus_tag="PKM27_10975"
     CDS             complement(28359..>29153)
                     /locus_tag="PKM27_10975"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNR07827.1"
                     /translation="NPINPTNPINPTNPINPTNPINPTMISIGNFFFKYRNLLFIVLY
                     LALFIPSPPLFSENRFGRGYFWWPIVFGLIITFKGQFIRALTIGLAYIVRGGKDGKVY
                     AEGLVTEGIFAHCRNPLYVGNILMLAGVGILANSLYYTLIVLPLFLFIYQCIVLAEEH
                     FLKNKFGEAFDEYCSRVPRWFFRLRGLYVTVRSMRYNWKRYLLKEHTTFFIWITGILL
                     IILFKYPHLTQHNAGFRNSILAWIIPLLAVAYGLIRYLKKSGLVRE"
BASE COUNT         7180 a         7182 c         7543 g         7248 t
ORIGIN      
        1 tatttgcagg cattctggct tgtaatagaa aatctattgc atggtttggg tttaagtcaa
       61 atcataaaat agtaagtcta ttgcatggat ttggatttaa cggcagccgg attaattcat
      121 gatcaccacc tttaaattcc ggtttgtacc caaatcgaca aagttggaag tgaggttggt
      181 aatggcgacc ttgattatac caggttctga aacaaaggtg gtcatctgac aaccatttag
      241 gtccaccgga atggacccga tgacgacgga ttccatggtc gccccttcaa cggctatggg
      301 tggagataac caggtcgtga atatactgat gataccggga ttaatggtgt cggccactat
      361 gctttgaata ctttttattc ccggagaaag gatgccggaa gatatcagta cggagtcggc
      421 ggtaatggta accttaactc cgtccttacc ggcaagggtt tttaattttg gaagggaatc
      481 cgtgatgcca accggaattt gcccgtccgt ggctttgccg agtgcatgta tccccccacg
      541 gtcattgccc aacaaaaggg agttggcagc cagcgaatcg gcgcctgttc caccatacat
      601 gacaggaaga atgccggtca ctttggaagc cagatcaatc gtggaatccg caaggtcctc
      661 atttttcaag gaaccatcga ggattaaccg ggaatcgatt gccccggtgt tgaaatcctg
      721 gttgatgagg gaagaatccc ggacaatgcg ggaatcgaca ctgccggtaa gcaggtcctg
      781 gttggcaatg gatgaatcca gaatggagcg ggaatcgata ctgccggtgg acaaatcctg
      841 gttgaggatg gtggagtcca ggagggtccg ggagtccaca ctgctggtcc ggaggtcctg
      901 gttggccagg gtggaatcct gaatgatacg ggagtccaca cttccggtaa gcaggtcctg
      961 gttggcaatg gaaaaatcca gaatggagcg ggaatcgaca ctgccggcgg acaagtcctg
     1021 gtttatgacg gaagagtcga ggagggtgcg ggtatcgaca ctgccggtgg acaaatcctg
     1081 gttgaggatg gtggagtcca ggatgatccg ggagtctacg gcgccggtcc ggaagtcctg
     1141 gctggtgatg gtggagtcga ggatggtccg ggaatcgaca ctgccggtcc ggaggtccct
     1201 gttgaggatg gtggagtcga ggagggtgcg ggtatcgacg cttccggtcc ggaggtcttg
     1261 gttgagtatg gtggaatcta atattttaag ggtggtgatg gcatcctctg ctatatcatc
     1321 cgtatagaat gaatagggca cggccatgaa ttgctggttg cttaccagtt tataattccc
     1381 gtcgtttttg atggccaatt ccactttcaa atactggtcg gcctcgatcc agggaatgtc
     1441 caacatccgt gggtagggag cggctccggt ggacgttcct tttccgatga tcaatgtaaa
     1501 taatccgaat ttgtcggtgg tggtcgtatg ggtttcttcc cattgcacag ggccattcag
     1561 tcctttagtg atggcaaaac ggacgccaat ggctttatta taaagaggtt ttccggaaat
     1621 gtccgtaccg acgatttcct tgccggtttc atccacggca acggcctggt aataaattcc
     1681 gtttaaattg ccaattttct gggccaggac atttggtatt attcccatgg ataataaaat
     1741 acctatataa atcagctttt tcattttaaa tctttttaga atttgacctt aattttctgt
     1801 caatgaaatg gccactccca ggctcaatcc gtaggcaaaa tttttcgttt tttgcccggg
     1861 gtccttttca atattggtga ggcctttgaa ataattcagt tcgagataac ttgagatgta
     1921 atccgacagc ttgatgttga caccgggaga aaagatcaat ccgtaatcga tgttgctcag
     1981 caggtcggat tccgtgatgt tgaggttttc attgtgcgtg gtttgggcgc cccggagcag
     2041 gaaagcataa tatcccgaag ccataaaata aggattgatt ttttcaaatc gggtcatgta
     2101 cccgagcccg agttttattt ccgcatattg cagcctccag gaataattct ggtcgtcgta
     2161 aaccaggttg gctcctccgt ggcgtacgcc gatgccgggt ttgatgatca atccgatgtc
     2221 attcacataa cggtaaccaa tgccatagcc cccggtgtat aaaccctggt attccttgtt
     2281 gtgggatacg cccgcccggt ccttgaattt gaaggaggaa aacaactggg aagcttccag
     2341 gaccaatccc gactgagcaa gccctgttaa tgagcaagcg gcaagcaggc agagtacata
     2401 aatggatttc aagattttca taatgtattt atttggaaat gattaatcgg atggttttta
     2461 cccgtcgccc gggatcctgg atggtgatca tatacacccc attggcccag gatgcgcatc
     2521 ggagggggtg tgaagtcagc ttcattacct tttccgaatg aaggaatctt ccgttcacat
     2581 cggttacgga gatgatcgct tgttcgattt ccgcatcact ggccagggtg acggaatgac
     2641 tggccgggtt aggatagact gttatgttta aagtattcag aacaggtgca gggatctgca
     2701 ccacatgata acgggggcct tgctgaaatc ccggtgaaac ggtgatttcg tttggatgga
     2761 ccgtggtatt ataagcctgg cctgcggtct ggcttatgga caccgtttca gtttgggttg
     2821 aagaaccgta ggccggtatg ctttgccttt tgatcgattg ggcatggacc ggtcccgata
     2881 acaggaagag caggacaata cccggccctg ccacaaacag gattggtttt tttttcatat
     2941 ataacggatt atgatgagtg cattcaaaac aggtcagccc ggttaccgcg agaatttgtc
     3001 ccttggtttt cctactcctt gctgaccaaa aaatcgaaat tcttaatcag ctcttgttca
     3061 catcaccatt attgaggacg aacctgtagc tgtagtcagg cattcttgtg ttcttttttt
     3121 ctaggtcaaa accaaaatac aaaaattccc aatcataact gatatacaga caaaaaatgc
     3181 cgggaatttt cttccgcata cttattgatt tggtctataa atttataaag attattaatt
     3241 tgatttattt ggctccttcc gctaatattg catgggcagg gagagtcctt ttgaatactg
     3301 ggctgcgggg tgaaccagct tttgagtcat accgcaacgg aactttttga gcgatgcgtt
     3361 cttgccatta ttcattttct tttatctaac ccgtaattca accaaatatg gaaaaaaata
     3421 catccgaagc ccttgcttca ccgaagcaaa atggtcaaag caagtgtccg ttccatggcg
     3481 gtgcgctcaa acaaaccgcc ggcggcggca cccggaaccg cgactggtgg ccaaatcagt
     3541 tgaagctcaa catcctgcgc cagcattccg ctttatccaa tccgatgggc gaaaaattca
     3601 actatgccga ggaattcaaa accctcgacc tgaatgccct aaaaaaggac atttttgaac
     3661 tgatgacgaa ttcccaggac tggtggccgg ccgactacgg tcattatggc cccttcttta
     3721 tccggatggc ctggcacagt gcgggcactt accgcatctc ggacggccgg ggcggcgggg
     3781 gctccggcag ccagcggttt gcaccgctca acagctggcc agacaatgtc aacctggaca
     3841 aagcacgctt gttgttatgg cccatcaaac agaaatacgg ccggaaaatt tcctgggctg
     3901 acctgatgat cctgacggga aattgcgcgc tcgaatccat gggcctgaaa acttttggtt
     3961 tcgggggagg aagggaagac atctgggaac cggaggagga tatctactgg ggatccgagg
     4021 gcaagtggct ggacgataaa agatactccg gggaccggga actggaaaat cccctggcag
     4081 ccgtccaaat gggcctgatc tatgtaaatc ctgaaggacc caatggaaac cccgacccgc
     4141 tggccgcagc ccgcgacatc cgagaaacct tcgggcggat ggctatgaat gatgaagaaa
     4201 cggtggccct gattgccgga gggcatacct ttggtaaaac acacggcgcg gccgatccca
     4261 gcaaatacgt aggcccggaa cccgcggcgg ccggtatcga agaacaagga ctcggctgga
     4321 aaaacagctt cggcagcgga aaaggaatcc acaccattgg ttcaggcctg gaaggagcct
     4381 ggaccaccac gcccacgaag tggagcaaca atttcttctg gaacctcttt ggctacgaat
     4441 gggaactgac taaaagccct gccggtgcgc atcaatggaa accaaaacac gggatgggtg
     4501 ccaataccgt accggacgcc cacgacccgg ctttaagaca tgcgcccacc atgctgacca
     4561 cggacctcgc cctgcgggtg gatccggcct acgaaaaaat atcgaggcat tttatggatc
     4621 atcccgatga gtttgcggat gctttcgcca gggcctggta caaactgacc caccgtgaca
     4681 tgggaccgat ttcccgttac ctgggcccgg aagtgcccaa ggaagtgctg atctggcagg
     4741 atccggttcc agcggttacc cacaaactgg ttgacgagca agacatcgag gcgctgaaga
     4801 aaaagatcaa ggattcaggt ctgaccatat cccaaatggt aactaccgcc tgggcctctg
     4861 catccacttt caggggttcc gataaacgcg gaggagcaaa cggtgcccgc atccggctgg
     4921 cccctcagaa ggattgggaa gtaaacaacc ccagccaact ggcaaatgtg ctgaagaccc
     4981 tcgaaggcat ccaatcggaa tttaataaat cgcagtccgg cggcaaacag gtttcattgg
     5041 ccgacctgat cgttctggcc ggctgcgccg gaatcgaaaa agcggccggg aaggccggtc
     5101 attctgtcac cgttcctttc aaaccgggaa ggaccgatgc ctcccaggaa caaaccgatg
     5161 ttgaatcgtt tgccgtactt gaaccggcgg cagacggatt ccgcaattat ctgaaaccgg
     5221 gtcaagtcgc ctcagctgaa gaaatgctgg tggacaaggc ccaactcctg aacctgaccg
     5281 cacccgagat gacggtgctg gtcggaggca tgcgtgtcct gaacgcgaat ttcgatcagt
     5341 ctaaacatgg ggttttcacc aaacatccgg aggccctcac caacgacttt ttcgtgaacc
     5401 tgcttgatct gaacaccggc tggaacgcaa tatcggaagc caaggatgtt tttgaaggcc
     5461 gggaccggaa aaccggtgag gtcaaatgga ccggtacccg cgtggatctt atcttcggtt
     5521 cgaactccga actcagggcc ctttctgaag tctatggatc ttcagacgcg aaagagaagt
     5581 ttatgcagga ctttgtggca gcctggacca aggtgatgaa ccttgaccgc tttgacctgg
     5641 ccgattaaca gagttgaatt attcatatca caaggggtgc ctggaaacgg gtaccccttt
     5701 ttattttatc acccggtgca atacggaaat tcttcgttcg attaccatta tgcttgtggt
     5761 cccagcccgg gacctggatc cggatgaacc actgtcctga aggttaaatg atatcctgtg
     5821 ataatccggt acccattcgc tgaccttccg gtttatcctt caaattgtaa ctttcgctga
     5881 agaagcaaag cggaagatga ataaaaccat ttttatcctc ctggttttcg tttattcctt
     5941 ctcagcaggc caggattctt ccggcaagct ggacagcgaa ttccggaatc ccccaaaaga
     6001 taccaggccc tgggttttct ggtactgggt cgatgaaaac atttcaaagc ccgggatcac
     6061 caaagacctg gaagccatgg cgagggtggg cataggccag gcgctgatcg ggcatatctc
     6121 tccaggtccg gtccgcgggg atgcccggat gctcagtaag gaatggtggg atatggtggc
     6181 ctttgccgtg aaagaagggc agcgcctggg tgtggacatt ggatttttca acggaccggg
     6241 atggagccag tcgggtgggc cctggatcag cctggaccaa tccatgcggc atgtggtaag
     6301 cgacgaatgg agggttcggg gcccggccct tttaagagaa aaactgcacg tgcccggacc
     6361 tgggtttaaa caaattgccc tccaggcttt tcccgtggac agcaggtccg caaaatcccc
     6421 gggcccttcc gatatgattt ccttccaggc ccgaccggaa accggaaata tttacaagtt
     6481 gtttgaccgg gatacggcca ccagctattc tttcccggcc ggctctggca gtaatgaaaa
     6541 aattaccttg gacttagagt ttaaagaacc ctggaccatt caggcaatca aatttgatta
     6601 cccaccggtt tctttttata cacaggtgga tttccagtct gctgaaccgg atgggatgta
     6661 ccgttcgatc agaaaattta aaatcgaccg gcggaatatc cagtttcaga tcgggcctaa
     6721 acgatttgaa cccctgtggt ttgccgtgcc ggctacccgg tccaggcggt ttcgttttgt
     6781 tttcgatcag atccgggcga ccgacagcct taggtttaaa gaaatagaat tatacaccgg
     6841 aacggtcgtg gaccagcata tcgacaaaca attgggtaaa atggcttccg acccggtccc
     6901 tctttgggac gcctacctgt ggccggaaca agaggaacca ggcaacggag acctgatcaa
     6961 accggaaagc ctactggatc tccgatcccg ggtggatgcc gaaggttttt tgaattggga
     7021 aattcctccc ggggattggg tcatcctcca ctcgggcatg gcgcccaccg gggccgtcaa
     7081 tgtacccgtg ccccctgaag cgacagggtt tgaatgcgat aaattttcga aggaagcgat
     7141 aaagacccat ttccaggctt ttgccgggca atttctgaaa accataccca gggcagaaag
     7201 gaccgccctg aagaccatcg tcatcgacag ttatgaagtc ggatcccaga attggtcaaa
     7261 cgattttgca gccctatttg ctgaaagatt cggatacgat cccctgccct ggttgcctgt
     7321 atttgccggc aggatcgtta aaagcgcaga cctctccaac cgctttttat gggatgttcg
     7381 gaggttgggc gcggacctca tcgctgaaaa ttatgtaggc cacctccgga aactttccaa
     7441 tcgaaacgga ttggacctct ggctggaaaa ctatgggcat tggggtttcc cagccgagtt
     7501 tttgcaatac ggaggccggg ccgatatggt ttcgggggag ttttggtttg aaaacccata
     7561 ttgggacctg ggccccatcg aatgccgggc cgccagttcg gcagcccata tttatggaaa
     7621 aaacagggtc tttgcagaag cattcaccgc cggtttcaat ttcaaacaat atccggcttc
     7681 gatgaaaagc cggggtgacc ggatgttttg cgaaggcatc aatcattttg tcctccacct
     7741 ttatattcac cagccctggg aaaaccgggt cccgggtgtg acggcctggt tcgggatgag
     7801 tttccagcgg aacaatacct ggtttgagca gagcaaggca tgggtggatt acctgcgacg
     7861 ctgccaatat ctgctccggc agggcgatcc ggtgagcgac ctgtgttatt ttacgggcga
     7921 agatgttccc aaaatgaccg gagccctgaa tccggccttg ccgtcgggtt atgattatga
     7981 ttttatcaat gccgaggtcc tggagcgggc ccgggccagg gatggaaaaa ttattctccc
     8041 acatggaaaa gaataccgcc tgctcatcct gcccgaacaa accaccatga ggccggcaat
     8101 gcttgaaaaa ctggaaaaac tgatagcgga tggggccgcc gtttacggac ccccgccttc
     8161 ccgatcaccc agcatggccg gttatcccgg ttcggaccag gagatcatgc gcctggccat
     8221 gcggatctgg ggcaataaag gaacagttac tgcctcaggc cggtcatacg ggaaaggaaa
     8281 actgtaccgg gatcaggacc ttgccggggt gttggaggac atgtccctgc ctccggatgt
     8341 cctcatggcc gacaccggga tcctgtggac ccaccggtca tccatgggcc tggacattta
     8401 ttttctttcc aacctggatg accgggaaaa aaagtcctgg atcagcttcc gggtaaaaaa
     8461 ccgggttccg gaactttggg accccgcttc gggaaggatc tccgaactgt cttcataccg
     8521 gatggaggga gaaagaacca aggtaccggt atgcctggat ccatcgggtt cggtttttat
     8581 cgttttccgt aaagccggcg gagaggccgc agaaccaaaa gcacggtcaa cccatgcagt
     8641 aaaggagatt gccctggatg agccctggca attgcatttt ccgggaggat gggatgcgcc
     8701 ggattccatc acggtcaagg acctgacttc ctggaccgat ttttcagata tgggtataaa
     8761 atatttttcg ggcacagcca ggtataaaaa cacgtttaat atcccggtgg aaaccctgaa
     8821 ggaaaacacc cgggtcatcc ttgacctcgg agaagtcagg atgatggccg aagtgaccgt
     8881 caatggcagg aacctcgggg tcctttggaa aaagccatat aaaacggaga tcacagaggc
     8941 tatccgcacc ggggacaacc aggtggagat ccggatcacg aacacctggt ggaacaggtt
     9001 ggtgggggat gcgaagtatc cggaagggct accgggcagc gaggtccatg ctccccggac
     9061 ttttgtgacg gtaaagggat ggaccgggga ggaggaactg atggattcgg gtttgatcgg
     9121 gccggtggtg gttaaaatta gggaagaatg atggaccggc tttaaaagga tcatgacgcc
     9181 ctggaacaac acatcagtcg acggggacga ctcgagggtc cggtacctac cgatataggt
     9241 tttgtacgaa tgagcccggg tccatcctta gtgacccaag tctttcaggc agcgagttga
     9301 gtcatcaaat tgtgcatgtc caggattccc caatgcgcga tatattttcc attgcgcagt
     9361 tgtacgatgt cgatcacctc catcaccacg cttttattcg tgggttttac cccgaaaaat
     9421 tcgcctttgt ggattgcatg aaatgatttt ctggtggtca ccttgtcccc ttcagcgatc
     9481 atctcgtgaa tttcgacttt caattcaggg aacgcgggtt tgagcagctg gttgaaaaaa
     9541 taaatcaccg catcgggtcc ctggggcgaa ccgggagggg cagtgtaatt gataaagtcc
     9601 ggtgcaaaga tttcatgcac cgtatttatg ttaccgcctt cgataaattc cttgtttacc
     9661 cggtaaacga tttttttgtt ttgttctgta gtcatgattt ttggtttatg aatgaaataa
     9721 cagaccaaag atgggtcgga tcccatttgc ggttattgtc cggaagcgac tttgaatgga
     9781 actattccga cgcgttgcga aattccaaag gggtttgcct gcacattttt ttaaagaacc
     9841 tgttgaaatg tgggggatct gcgaattcta atttataagc aatttccgaa acgctggatt
     9901 gtgaatgcag caggtagact tttgcttcca tcagcaacat ttcatcgatc aggtcgcggg
     9961 cagttttgtc ggagcaggtt ttgaccgccc ggttcaaatg gtccggcgat acatgaagca
    10021 gatctgcata ttcggctact ttgcggacgg tgagaaaatg ccggctcacc agtttcaggt
    10081 aggcgaaaaa aaggttttgc gccgggttgg agctgccctg gctggagagc tggaccgcat
    10141 catagtcccg gctggcgtgg atgaggatca gctggatgta taattttatg gtctcactca
    10201 aatgggtttt ccggttcctg acctcatcgt ttattttgaa gattattttt tcaatttccg
    10261 acgcggtttc ttccttcagt tcaaaacatt gcacccccga atggttgaga aacggaaact
    10321 tccaggtttt gttggcaaag agggaagtgc tggaaatgaa tttttccgtg aagagcatgt
    10381 aataggccag gaaatcgggg tttacatcgt aaagcgaaaa aatttggccg ggtattccaa
    10441 acaggatcca attgcgtacc ggccggtatt tttccagccc gatgtcaaac ctggcgctgc
    10501 cctggcgggt gagggaaatc ctgaaatatt cggtcttgat gtgttcaatt ttctttttta
    10561 catccctggt ggaataaagc ccgaagccat ggtctataag tccggaagaa ttgtccattc
    10621 cgaaatcact tttccccttt ccttctttat gaattttata cagttcggtg tccagtttga
    10681 cttgcctgat cccggtggaa acactcttat acatatccgg ttttactttt cggtttaaag
    10741 ccatccttgc ccgatatccg gtccaggtca ttccgaatgg ctgcccggaa tgcggatgac
    10801 cgcttaaaaa ttactttatt ttctgaatat ttccaagagg gccgtgaccc caatctgcac
    10861 gcccagaggg atccccgcca ggtccacctg gtaatttccg ttgtgattaa aaaaaggcgc
    10921 ccgttttcct tcccggactg cctgggcata gagctccgga tcagcggttc cgacgaggat
    10981 gaaatcacag gtcgttttat tatttcccaa aaccaaatga taaaagtctt ccgaagccat
    11041 aatgggcgga gtgtcggaaa atatattttc aggaccgagt aggggtgtca aagcgttatt
    11101 tattttcctg accaggggag tatggttgac ctggggaaat acattgcttt tcataagtat
    11161 tttaggatgg agttcatggg gaaggccgtt ggctactgca aggccctcat ttatcctttt
    11221 tatgccctcc aggagggtat ttcttgtttt ttcagtaaac caccttaggt tgagtttggc
    11281 cactgcataa gctggaatga cgttgttatc tgaacccgct tgaatggaac ctacggtcaa
    11341 tacccccaca tcctgggcag ggagactgcg gctgatcagg gtttggtact ggaggacggc
    11401 attgctggcc atcacgatag gatctatggc caattcaggt gtggaaccat gtccgcctat
    11461 cccataaaaa gtcacatcaa gttgatcgct gccggaagtc cgctcgccgg tgccgttaat
    11521 gatacttcct accgcaatgg gccaggtatg catcccgaat aaataatccg gtatgggaac
    11581 acccttttca tacattttat cgttcaccat ggcctttgcc ccggtcatgg gttcttcggc
    11641 tggctgagcc agaaaaacca gggtcccttt ccactggtct ctcagtgcca tcatgatccg
    11701 ggcgatgccc agcatccagg tgacatgcgc atcgtgtccg caagcatgca tgaccggtac
    11761 ttccgtacca tcttcttttt tcatggtttt gacgctggca tagggcagtc cggtggtttc
    11821 ttttacggaa ttgcagtcca tatcggcccg gtacatcaca atgggccctt ccccattctt
    11881 taaaacacct acaactccgg tttttgcaat cccggtaatg acttcaaaac ctaaagtctt
    11941 taattcttta gctacaatcc cggaagtacg aaattccaga aatcccaatt caggattttg
    12001 atgcaggtct ttgaatattt ccaccagacg tggggagtct gcataagcca tttccttgac
    12061 tggtttggag gtctgagcgg gaagggttag aattgccaac aggcatactt tcagaagaat
    12121 gaatgtcttg gtcatggttt tcatgctgag atttttgagg atgaggatga ggttaaggat
    12181 gaggttaagg atgaggttaa ggttaaggtt gaggttaagg ttgaggttaa ggttgaggaa
    12241 cttcaatcct taaaatggtt ttttaaaaac cgaagcccct cctgtgcaaa gaaatcctgg
    12301 ttctcagcca ggtttttcca gatgcaaacg gcgccggcca gttccttgat ctcgggagta
    12361 aaactttcaa tggagagggc gccctggtat cggatgtttt ttaatccccg tttcagggaa
    12421 tcccaatcgg tttgcccggt cccgggggta ccgcggtagt tctccgaaac ctgcacatgg
    12481 atcaatttat ctccggtcag ggtaatggcc tcctcgaggt tgcgttcttc gatggccata
    12541 tgaaaaccat ccagcatcac tttggctgcg gggtggctaa cctcctttac aagacgcatc
    12601 acgtcttcag ccgtattgac caggtcggac tcaaaacggt ttaatggttc cagggcaatg
    12661 gccaggccac gttcctgggc catacggcat actttgtgaa gattgcggac ggccaggtcc
    12721 cactccgccc tgcgctgttc cggaggaacc atccgcgcct tgcccaccgc cgagtacatg
    12781 gggccggcca ggaagccggt atcccaggcc gtgcaaaggt ccaggcattc acgaatgtag
    12841 gcaaaacagt tttcatgcac ggccgggtcc tcatgggtca ggtcgcgcgt agggccaaag
    12901 gccccgcaga cgatggcttt gaggccgtgg tccccgagtg ccgcctttac ctcttttgct
    12961 tttatttttt cagggtactc cacgggaatt tccaccgcat cgtaacccat cgatttaatc
    13021 tttgggaaaa gggatatggt ttccgaggtg aacggagaag tccacagcca ggtgctcacg
    13081 ccaaaagtca aattcatacc ggtgttttaa atcttttgtt taaatgattg agtcggagaa
    13141 gacggagaag atcctgattg cttttcgggg cttttattca ggagaaaaat ttgccacctc
    13201 atcagccttt ttagatcagc ggaatccggg atgatcctct cccttcactt catagccccg
    13261 ccacttgtat tctttagcca ggcgggatgc tgccggccag gctatctggt gttgatcgct
    13321 cgccacccat tttcttctgt tttcttccgt atcgaaagaa atttggatct ggtagttgaa
    13381 ggtcgtgggc tccgcctgga tggacttggc tacctccttg gggaaaagac gcaccaacct
    13441 ggatccgaga taaccttgct gaacggtcat ggccggcaca taaatggaat gatacatttt
    13501 ttcaaaggcc tcgctgtttt cttccggcac cacaaagtcc atttgcaaaa ccatggcctt
    13561 tggttttgcc ggattcgctt ctttattttg agaggccgcc acaccgatca ccagtaattc
    13621 gaggtccgcg gttcccgaat tcgacaaggc cagggtttca cccagggaac cggtaaaact
    13681 gtcgtccgca acgatgggct gggtggagga gttcaccgta agggtaccgc ttcccttaat
    13741 cacataatat acttcttcaa tcccttcaag acggcgaggc cctacggatt ttccggccgg
    13801 gatcaccagg tggtctacat gattccagtg ggtgctgaaa atttccgggc cgaaggccct
    13861 tttgcagagc gccccctggc ctgcatacgg gtgctggttg tctttgagcc cctctttgtt
    13921 caatgaaccg gaaacaaaaa ccgggacgcg atcgatgctg gcgcctaccc ggttatcacc
    13981 caggtcaaaa gcatccccct gtcctttaaa ctttgacacc gcaaaattca gccaccggac
    14041 agggccatcg gttggattat agatggcatg tgcatccccc tgtttgcacg gaacggcaac
    14101 cggagcttgt agcctcgacg tgcgtccatt gatggtaaat tccgcttcac cgtcaaggag
    14161 aacatacatc tcttcaatct ggtgatgaaa gtggtgcccg atgcctcctt tggattcgat
    14221 gacgccggca tgcaggtaaa gaaagtttgt ggataacaca tcccgtccga tcagctgtgc
    14281 gaattgcatt tgtcccgcac cggcatgcac ggcgcggagc acccggtact gcgcaggatc
    14341 attatgcacg atgcgatcgg gtttggattg ggtaaaagac ggcagggccg tgatggaaaa
    14401 gaaaaacaaa aacagtctgt tcatataacc ttaagatttg aatttaagaa taattcgtta
    14461 ttccattaaa agaggctctc gccaggtgtg cggcaggatt caccatttca attatttatt
    14521 tctccctgag cccatacact acgtaccctc tgggcaaagc ccccggtttc agggagtgat
    14581 tataactatc cggtgcaaag gggccggtgg cattgatcac caggtattgt ctgccattcc
    14641 tttcgaacat ggagggctga gcattggatt cgtagctcaa ggtcgtttcc cacaggaggt
    14701 cgccgcgatc cccgttaaag gcgtacaact tgcctccctt agccgtggca aaaaccaggc
    14761 cggtggaagt aaccaccatt ccttttctca gcgtgccgcc cggtgctccc gtatttttat
    14821 ctcctttggt gaaggtcgaa tcttcgccca cgggttttcg ccatttgatc gttcctgtat
    14881 tcagatcata ggccagtagg gaggaccagg gtggccctaa caagccgggc caatccaggc
    14941 cgtagtccgt ggtataacgg atttcaggat gggggacgcc ttcaggataa tccctcatcg
    15001 gggcggatcg cttttcatca ggtgtaatag ccgcgccccc cgaagccacc accggcccct
    15061 ccatttcacc tcccgcatcc ggtcgtcttc caaagttcat tctgttcggg atgccgccca
    15121 aataccggta caaggccgcc agggtctgct catccaggtg aacaaatccc ggcatttggc
    15181 ccttgccgtt ttccatgacc tttttgaact cgtcgtataa aatccgctgc ccggcgttta
    15241 ccagggcggg cccaaggcca ccttccttgt ttttaccatg gcaggtttga cagctgttga
    15301 tatatagacg gtttaattta tttatttcaa gggccgacaa actggcctgt ggaggcaata
    15361 ctttgttcag cttgtaaatg gatgcatgtt cctgggtcag gatgtacatc attccctttt
    15421 ccggatcgga tgccgtgttg ccaaagtttg cccctcccag ggctccgggc atcattacgg
    15481 tttcatactg atctgaagga ggaatgaaca atcctgaccg ggccgaatcc agccgcttca
    15541 tccattgttc ctttacctcg gcggagagat aaggattgag ggtctctttg gttactgcat
    15601 gacgggtaaa gtccgggacc atcacaggga tgggttgggt gggccaggct ttttcgcccg
    15661 gcatggcgct ggcaggaaat ggtttttctt caatagggaa aaccggttca ccggtgaccc
    15721 ggtcaaaaat aaacaggaaa ccatgtttgg ttgctacagc caccgcatcc tttttattac
    15781 cctcccggat cacggtgatc aactgcgggg cggaggcgag atcgtaatcc caaaggtcgt
    15841 gatgcaccgt ctggaaatgc cagaggcgcc tgccggtgcg ggcgtccagg gcgacgagac
    15901 agttgccgaa aaggttgctt ccttcacggt ccgcgccgta ataatcgtag gttggagaac
    15961 ccagcggcag gaaggcgatg ccccgttttt tatcaatgga tatttcactc cagacattca
    16021 ccgctcctac atatttaaag gcgtctttcg gccaggtgtc gtacccgtat tcgccgggcc
    16081 gggggatggt atggaaggtc cactgcaatt tgcccgttac gacgtggtag gccctgacat
    16141 atccgggggg cgaaaaatag ttttctccgg gggccgaacc aagcaggatc aggtcttcaa
    16201 acagtgcccc cggcatcatg gcctgcattc gggtgatgga tgaagggtca cggtccaggc
    16261 cttccctcag gtccacatat ccgttgttgc caaaactaag aatggatttt ccggtcgtgg
    16321 catctatggc ctgaagcgta ttgttgaggg taaacagcaa gcgtttatcc cgtttatctt
    16381 cactttccca gtagttgatg cccctcctgc tgattccctg aagccggctg tgtatccaga
    16441 tctctttgcc cgtcagggca tgtactgcga tcagcgaaaa atttttagcc aatacgtaca
    16501 tgaaggtgtc gacaataatc gggctgaaaa aataagcctg actgtcagag gccggatata
    16561 cccatttcac ctccaattgg tctacattct ttcgggtaat ctgtgagcca ttgaaaaacc
    16621 gcgactgatc cgggtcccct ccatatacct gccaggtatc gtgaccggtt gacgcggggc
    16681 cgggctgaca tcccgcataa aaaatcagga atgcggtgat gaggagggca tatctgatgt
    16741 gggaaaccgg tttaaacatc aggcaaaaga agattggtat ctgaattgga tttttggaat
    16801 aaacagggag atcaaagaga tggcgccttt actttcataa tgccgttcat gatcagccaa
    16861 tgcccgggaa aagtgcagac ataggggtaa tccccgggtt tttccggtgc gataaatttt
    16921 aacttcactg tttcttgcgg atttaccagt ttggtggaaa acaacacttc cggcaggtcg
    16981 ggaacatatt gccgctcggc gccctgcggg tcggcagcca acttgtcggc tgctttaccc
    17041 acggtttcca gggacccgat ctgggtgatc aggaggttgt gttgcatgaa atcgggattc
    17101 tcaaacacga tttcaaccgg ttttcccgcc tccacggaaa aggttttctg gtcgtacttc
    17161 atttcattct tgatcgtttt gaggtggatc agaatggctt ccccggagtt ttcgaccagg
    17221 tctggggctg tgcccggcag gctgcccagg tatgcctcac aaaaggtctc cccaatggat
    17281 ttatctgaaa acagggtttt ggcagggccc tgctcctggg ccacttcata tttccattcg
    17341 cctgcaagag accgtttctg atcccctgcg gccaggaaca attcttcggg tttgccgtag
    17401 atgcctcccc gcccgccaat atcttccacg cggatggcta tgacattttt ccccggtttt
    17461 aagatccctg cagccacttc ataaagcctc ttgtcgaggt gcctgctttc cgtacttccg
    17521 acccttacac cattgatata ggagatatcc gaatcgttga ccggcccgag cgaaagcttg
    17581 gctttttttg ccatggatac cggtatgtca agggtttttc taaaccagat cagcccgtcg
    17641 atatccaaac cggcatcttc gatgaaagtg ggcaggatca tcttattcca ggattcatca
    17701 tcataagtca gggactccct gttcctcgat ttcacctggc tgggatcata tcccggatga
    17761 tcggcaagga aagcctgcag gaatccattt ttatgacgta ccgcggccac atagatggcc
    17821 ttcgaaagcc actcgtcccc gataacgctt tcttccaggc tcattttata caaaacattg
    17881 cccagggaat cggaaggctg gagttctgcc agggaaagaa tggccgccat acgggtattg
    17941 gggtccggat cgttcaaaag attgttttta atcacggttt cttcccgcca accggctttg
    18001 gacaaaatct ggatggccgc cttacgcacg cccggggagg gatgtcccag tgattccaat
    18061 actacggatt gggccttgtc ctctttgctc aggtcaacca ggccatcgag ggtccataaa
    18121 gcatggatgg cggcataacc gtttccatct ttattttccc tggccaatct atacaatgcg
    18181 ggaaggatgt ccggcttgcc gcgctccacc aggaggcgct gggcggtgag gcgccagagc
    18241 atgtggtcat ggttcagggc agcgatcagt ccatccggat ccccgggatt gagccgcatg
    18301 ggtttataag gttttgcctg ccggctcacc acccgccaga tgcggccatg ggacttgtcc
    18361 cgaagcggat tttcataggc gttgcctttc ccgttctccc ctgcaaaacc accgcgttcc
    18421 ggcgtggggg tcggattgtg ctgaacgata aaattgtacc agtccaggac ccataccgca
    18481 ccatccggcc ccgtctttgc gtccacaggg gaaacccatt cgtcagcgct tgcaaacaag
    18541 ttccatccgt ccttttcaat gaatcccgcg ccgtcctttt ctattttggc caggtgcacc
    18601 aggtgcccgg tgggttcgca gaccagcgcg gtgtgatccc aatattcccg gggataagcc
    18661 cttgccgtat aaaaattatg cccggaggct gccgtaaagc cgccaaatac atcgacctga
    18721 cgcacatgat cggtgatcgt acgcatggcg taatggccat caattttgct gctgcccaac
    18781 ggtcgtatcc cttgtacatc cttcatggtt ttggctggaa tgcccaggaa gacgctgtgg
    18841 gtattattgg cggtggaggc gaatacgtca ttattgggcg ttattccgag gccccaggtg
    18901 ttgttgctcg taccggtcat aaattcaaaa ctgctcagat ccggtttgaa ccggtaaatc
    18961 ccctggctga atttgcggtt ctccccggcg atgacgccgt cgaatccgga gtagccaacc
    19021 actccccaaa tctggttatc gaggccgtat ttcaggttgg aggggcctgc atgggtgtcg
    19081 aaggtgcccc agccttccat gattattttc cgctcatctg ccttatcatc cccatcggta
    19141 tctttcagga aaataaaata aggggcctgg gagaccatca ctccgccatg aatgaaaacc
    19201 atcgaagtgg gaatattcag gttatcggca aataaagtaa acttgtcggc tttcccgtcc
    19261 ccgtccgtgt cttcgcaaat tttaatccgg tcatctccga tggtacggtc ttctcttacc
    19321 gtgttgggat agtcaaccgt ttcgatcacc cataatctac cccgctcatc ccagtccatg
    19381 gcaatggggt tgatgatatc cggctcggaa gcaaaaagtt tcaggtcgaa accaacgggt
    19441 acctggatca gttttgcgga ttgttcggga gagaggggct cctggaagcg cgggcccgga
    19501 actctttttt cgtaattggg aatgcccggt tcatcgcgat aaaccagttg agggatttcc
    19561 gacttaaact tttcccattt caattttgcc tcgtctccaa ccgcccaaag caatccttcc
    19621 ttgaccaact gttggaaacc gggatggccc caggtccttt catcgtgtcc ataggccgta
    19681 taaaatacac gcccttgacc atgggtttta atccaggtcc agggttcacg gtggtctcca
    19741 tccacccgtt ccatcaatat ttcgatgtct gatgccagtt tgtcgtgaac ataggtttcg
    19801 tcccaggttg agaatgcact gatattcttc atgaccggat gctcgttttg gatgattgcg
    19861 gcggtgaagg tgtcggtccc gtgattttta aactgtccgc ccaccaggtc gatgtatttg
    19921 ggcgaattct gaaaacaaaa actggcgcag tggatgggta caaagccccc acctttattc
    19981 acataatcca gcaaggcggt ctcctgggca ggggtgattt cctcatgatt ggcgtagatc
    20041 atcagggcgt cataaaggcc aagggttttt tcgttcagat cgtccgggtt attggtgtag
    20101 gtaaagaata tgccctcctt ggaaaggctg gaagcgagca ggggaaggaa ccgggcggaa
    20161 ttatgatgtt ctttttcatg gcccagaaaa aggatttcca gttttcctga gccgtcacgc
    20221 aggctcgcgc ccggggaagt gcaggacggg atgcccagga tggttgccag cagataaata
    20281 caggtttgtg ccaatccttt catggtctga gatgtttttt ttagggagag attatttaaa
    20341 cagtatttgg gcaaaggaat agaggttatt cctccagaca ggccaggtat gcccgcccgg
    20401 gtattcactg taaacatact ttaccttcag ctcgtcgaat ttttccatca tgattttaca
    20461 gttgttatag gcaatatctt cctgcccgcc catggatacc caaagcgatt tcagatgggc
    20521 attgatctcc ccggcgtttg ttttcatgaa ttcatactgc ggattggaaa gttccgggcg
    20581 gttggcaaac caaccggaac tgaacacgcc gagccaggag aaaagattaa aattccttat
    20641 ccccgcgtaa agggtttgca ggccgcccat agacaatcct gcgagcgccc tgttttgggc
    20701 atccgttttt acccggtaat ttttttcgac aaaaggaatg accgattgtt tcaattcatt
    20761 ttcgaaggac cggaggatct gttcattgaa gcctgcaagt cccccgcttc caatattgcc
    20821 atccagcatc accaccagca tgggtaaggc ttttttttcg gcaaggagat tgtccaggat
    20881 gagatcggtt ttgccctggg cggcccaggc cctttcatct tctccaccgc catgcaacag
    20941 atagaggacg gggtatcgtt ccgtggcatt ttcgtcgtaa cccggcggcg tataaatggt
    21001 aaaacgcctc caggtttgca gagaggggga ataataacgc ttcgacctga gttcgccgtg
    21061 cgggacctct tttatagcat agtagccacc ccccgcaaac gggatttcaa tgccgctggc
    21121 catgcggccc ataccataaa atgcctcgct ggccgggtcg gcaagggcta ccccgtcaat
    21181 cagcaaggaa taataatgga atccttcgcc gagggaatcg gtggtcacca tccagtaccc
    21241 ccctgtatcc ctctccatga catatcgttt acccaggttg atttcgactt tctgggcatc
    21301 cggcgctttg atgcggaacc ggacccggtg atcaggcagg atctgcggat acttcgcgga
    21361 gcgtatattg gtggaagccg gaaatcccag gacggtatat cttgaaaagg tggagggatc
    21421 cacgggttta aaaagaaact gggaaaacat gtacaaaccg tttttccaga ccttaaaatc
    21481 atggactccg ggttcgaggt aatagatatg gggaacccct ttttcaaaca ggtagtcgtg
    21541 ggtgcgttgg ctcacccgga gcagaccatc gttgtccccg caggaaatcc aaagcaattt
    21601 taattgtttg ctggcggcga caggatcagg caggagatcc tctggccgtt tggtattggg
    21661 ggccgatgaa aatccgccta cccaggcaaa ttgatccagg ttgcccagcc cgaaattcag
    21721 ggactggccc cctcccatgg aaaggccggc cagggcacgg gaattccggt cggtcagcac
    21781 cgggtattta ttttcaatga aggggatgag gtcgttcaat aaatcctttt caaaagtggc
    21841 gaaggcctgc actttcaggc tgtcaaaaat attcccgccc gcccggtcgt ccttcatggc
    21901 gcggccgttg ggcatgacca cgatcatcgg ttcgatttta tgctctgcat aaagattgtc
    21961 caggacgata tcgggccggc caccccgcat ccattcgtat tcatcccctc ctatgccatg
    22021 caacaaatag agcacgggat acttttttgc cgtggaataa ccaggagggg tgtagacgag
    22081 cgccttccgg ttgaccccga ctgtccgtga gggataaaag acggaatcaa tttttccatg
    22141 cgggatgttt tcgcgaagcc ggtcgaaacc ttcgggggca tagggttctg cgggctgggc
    22201 aaaagcgggt ggagccaggg ccacgaggat ggagaacaaa gaaagcaggg attttatcat
    22261 aatcaaaaat actaaagcaa ggccaaatgg gcataccgtt acccggcagg aacggatcca
    22321 ttatattcct ttccaggatt ccttgccttt acggtggagg tctatatttt ggaaaggtat
    22381 tgaaggacct ggtttctgcg ggacagggct gagcccgaca ctaagagcac agggacatca
    22441 aagtcccaga cccattcatg cagcagctca tttactcttt gcctgagttc tgatagttca
    22501 ttctccggac aagcgatgac atccggattt tcaatgggta cgaagatcag caggtcaagg
    22561 gtgctcatgg ccttaaaaac cctctggtac aaggtttggt tttgaacatg gcccaacgca
    22621 tagctcaact ggatataggc caggatatcc accgggcagc ggtcaaacag ggtacaattt
    22681 ccccctttgg cgatttgttc aatggaataa tccaattgca ggaagaattc atccagcccg
    22741 ggaagatctg aaaaatgatg accctgttct tccagttcat gataagcttc cgcgcggctt
    22801 gtatattccg gaagggattc cagcaggttc tctaccaggg tggatttccc aacccggtgc
    22861 gcacccgata cggctatttt catctttaat ctttatcccg gaccaagatc cgggtttgca
    22921 gtaaagttac ccgggtccgg ccataattta ttaaacgtaa ctgctcccgg actttgtccg
    22981 gattatggag caagtaagag gatgccattc ggaatacata aaccggaagg gaccgggaga
    23041 cttaaccgac ttgtccgttt ttgacgtgtg cttgtccttc ctgccgtacg gtaaggcttc
    23101 acctttacat ggtcaatgtt gacaaagaga atttaaaaat ttaatagagt atgaaaatct
    23161 ttaaaacctt gaaaggaatg cacaccgcgg gaaaggccgg gatgattttt ttattaatgg
    23221 ggtcctgtga aaaggtggaa ttcgaccctg ccctgaaaca tgaatttacg attcaatccg
    23281 ccgcagacgg gtccgaatac cggatagagg tcgggcttcc cgccggttac catcccggag
    23341 gcgaaaagta tccctccctc tatattcttg atggggaaga aaattttgat tttgtttcca
    23401 gtcagtgcag cgaaatttca gatatgcttg gcgttcagaa tgtcgtgacc ataagcattg
    23461 ggtatgggaa cgaccggagc ctggattata cgcctactga agtcagcgca gtgaccgggg
    23521 gcggtccgcg attccttcat tttattgaga ctcagctgat tcctgccatg gaggggtatt
    23581 tcagcgtgga tacctcccgg ggaagccgga ccattatggg tcattcctac ggaggacttt
    23641 tcggggctta tgcctttagt gtgaacaaca gattgtttgg caattacatt ctattgagcc
    23701 cgtccctctg gtttgacgac ctcgtttcct tacaatttga aaaacaatac cggacggaaa
    23761 acaaagacag atcgcagctg gtgttcctgg ggatcgggga agcggaaaat ttaggcagga
    23821 tgcaggcccc ttttgaagct ttttatcaaa cccttcaaag ccaatacccc catatcaaat
    23881 tgacgaaaaa caaggaagaa aacctcgggc acatgggttc aaggaatccc aacatcgtta
    23941 aaggcttaca ctattatttt caaaaccggt aaagccgcac cttcaatcaa tcctcttaaa
    24001 caaatatcat catgaactca ataaaaaata tctgcctgct gatcgcgatc ctgttcagca
    24061 tgcctttgac cgctcaaagg accttttcat ccctgtccaa ggaattcaat acccttccaa
    24121 aactgaccct ggagccgggc gtcggaatcc ataccaattt tggaacggat ttcctgatca
    24181 ccactctgat tcaatggaat tggctcgacc ggctgagttt cgcctcccat tcttccttca
    24241 atattaacaa tattacccaa cgttctttta atggcatcaa aaccaggtac aattattcaa
    24301 tcaatcaaaa attggggctg ggcaccacct tctacaccgg tgggaattcc cattcggttc
    24361 ttcttatggc cggtattaaa tatacttcat accgggaaac cctggaggat gcccggtttg
    24421 aaccgttcgg gacggccatc catgctttga gcccggatta cggattgatg tacagccata
    24481 agaaaatttg gaaaaaatat tttttcacct atcgcatgta tgtgccgctt tacccctggc
    24541 cggccaaagg atccgatctg ctttacctgg aagggaacat ggataatatt gcccttgagg
    24601 tgggcctcgg aatcagaatt atttagtgtc ggccgcaaaa tcaattcggg gcgcccttgc
    24661 cgtccccgaa agacaaacgg acacccgcaa aatttaactt tatttacatc accgtaaaat
    24721 cagacgagaa tgaaaaaaat ggtctttatc ataccgccca ccgtagaatt gctggatctg
    24781 gcgggtccgg ttcaggtgtt tacagaagcc attttctatg gtttcgagac ggaaatcgaa
    24841 ttttatacct accaggataa cccggtcagc acctcgggcc ttgggttcgg gaagatttcc
    24901 cattacagcg aagcgaaatt aaaagaagga gattatgtct ttgtaccggg catgcacgaa
    24961 gattatgtga gaagcatttc ttttaaggcc gaaaaagctt tttttaactg gttgaaggaa
    25021 tgttccgata aactcatcac ggtcggttcc atttgcaacg gggcttttgc tttggggcat
    25081 gccggcctat tggaccacac ggagtgcacc acgcactgga gaagagtaaa cgaacttcaa
    25141 acgcagtttc caaaggctaa agtggttgcc gatattctat atgtgagaag caaccgggta
    25201 tataccagcg ccgggatcag cgccgggatt gatttggcgc tggccatcct ggaggatctg
    25261 aaaggggccc tgtttacgca taaagtggcc cggggactgg tcgtgtatca ccggagaagc
    25321 ggtaaacata aacagcacag cgtatacctg gattacagaa accatatcca tccgcaggtc
    25381 catgaagtgc aggattatct tttggagaat ctgtcgaaag aaaacagtat tggttcgctg
    25441 gccgtccggg tgggcatgag cccgagaaat ctcagccggg tttttaaaga aaagacgggg
    25501 attacggtcc tggaatacct gaccaacctg cgcatcgaat atgcaaaaac catgttgaac
    25561 aacccggaat acaccgtaga ctatatcgct tccaaatgcg ggtttaaatc ggcgagacaa
    25621 ttgcaaagaa tcttaaaaaa gcaaaacgga aacaaatgaa taaccacagc gattctgcta
    25681 aaattcagaa ggcccggacc accggcttat tttttctttt ggctttttta gcctacgggc
    25741 ttgggcgaca tctctttaaa agcgaagggg gccctgaaaa ataccttggc gcggcactga
    25801 ttttttcgaa ctccatcatg gtccttttta ttggtctctt tttgagagag accctaaaac
    25861 agtctaacgt ttgggttggt aatatttatc ttatggccag agtttttgag gccctggcgc
    25921 tggccagtat cttattaaac ttaatgcctt cgctgaatat tccggacgat gccgggtact
    25981 ttcctgcgat gttggtttta ggcttgggca gtattccgat gtgcctggaa ttatacaaac
    26041 aaaagatctc cccagcctgg ctggcacttg ggggcctcat cggatacacc attttttcct
    26101 ttgggttttt aatggagttc ctgggtaaaa aatggagtat gtatttactg gcccctgggg
    26161 gtgcctggga aataactttt gccgtatggt taatgattaa aaatggaccc aataaaattc
    26221 atgatccatc ccccgctaag gccggatagc agcaagggcc attttgatct cgagggggtt
    26281 gccgtgttca ttgatccccg aaagaaattg aatttcccct aagcagtgga atcgcacccc
    26341 ccgcacattc tgcagatgca ggcgtcattt tgtcagttgc aggccctctg gcatgaggat
    26401 tgatcacccc agacattgaa gattaatcct aaaatcctat taatcctacg aatcctatta
    26461 atcctataaa tcctatccaa tgagtgaaaa aggccagatc gcagtccaaa ccgagaatat
    26521 cttccccatc atcaaaaaat tcctgtactc cgaccaggaa atatttctta gggaattgat
    26581 ttccaatgca atagacgcta ccacaaaact tcaaaccctg tcctccaaag gcatggcaaa
    26641 gggtgaactc ggggacctca ccatcgacat cctcctggat gaaaagaaaa agaccctgac
    26701 catccgcgac cggggactgg gcatgagcgc agaagaagcg cgcaaatacc tcaaccaggt
    26761 cgccctgtcc tccgcccagg agtttttgga aaaatacaag ggggaaacca acatcatcgg
    26821 gcatttcgga ctggggtttt attcggcctt catggtggct gacaaagtgg aggtgctcag
    26881 caaatcctat aacgaggagg aaaagcccat actctggacc tgcgacggca gcccggaata
    26941 taccctcaag gaacacaaga aaaaggaccg gggcaccgac gtgatcctcc atgtcaacga
    27001 cgatggaaag gactacctgg aaaaaaacaa gatcgaagag cttttgaaaa aatactgccg
    27061 gttcctgccc tatcccattc gctttggcac ccgcacggaa tacgactggg aaggggaagg
    27121 cgaaaaccgg aaagagatca aaaaggaggt ggacaatatc ataaacatca cccagccgat
    27181 ctggaaaaaa tcacccaccg aactcaccga cgaggattat aaaaaatttt acagcgacct
    27241 ctacctcttc agcagccctc cgcttttctg gatccatctc aatatcgact accccttcaa
    27301 cctgaccggc atcctctact tccccaaggt gaccaccggc atggactggc agaaaaacaa
    27361 gatccagctg tacagcaacc aggtctatgt cacggatgaa gtgaaggaca tcgtgcccga
    27421 tttcctgatg atgcttcatg gcgtgatcga cagcccggat attccgctca acgtttcccg
    27481 ttcctacctc caaagcgacc gccaggtcaa gaagatcacc tcctacatca ccaaaaaggt
    27541 ggccgacaaa ctggaggaat tatttaaaaa cgaccggccc tcttttgaat ccaaatggca
    27601 ggatatcggc ccgtttgtaa aatacgggct gatcaccgat gagaaatttt atgagaaggc
    27661 catcgggttt tccctgctca aaagcgagga tgggacttat tacaccccgg aagcctaccg
    27721 cgaaaaaatc aaagaccagc aaacggataa aaacggccag gtggtttacc tctataccca
    27781 tgcacccgaa ctgcatcatg cctccatctc ggcagcccgg gccaggggtt atgatgtgct
    27841 cctgatggac cagcccatcg acaaccattt tatgcagcac ctggagatga agatcgaaaa
    27901 gacccatttc acccgggttg attccgccac cctggacaaa ctgattgaaa aagaggacaa
    27961 gatcgaatct gtgctctccc aggaggagca agataaggtc aggactttgt ttgagacctt
    28021 cacccaaggt accatgaaca aggtggaggt ccaggctttg tcgccctcgg acgccccggt
    28081 ccacatcacc aagccggaat ttatccgtag gatgaaggaa atgcagatga tgcagggtat
    28141 ggggtcagac ttatttccgg attcttacca ggtgatcgta aacggaaacc atccgctggt
    28201 cgcggaaaaa ttgttgcatg cgaccgatga agccaggcag aaagaactgg cgacccattt
    28261 gtaccgcctg gccttgttaa atcagaattt gcttaaggga gcagaactca cggattttgt
    28321 gtccaaaaac ctggaagctt tgtcccgcaa tgattagttc actccctgac caggcctgat
    28381 tttttcagat accggatcag cccgtaagcc acagccagca agggtatgat ccaggctaat
    28441 atcgaattcc tgaaaccggc gttatgctgg gtcaggtggg ggtatttaaa caggatgatc
    28501 aacaggatac ccgtgatcca gataaaaaaa gtggtatgtt ccttcaagag gtatcgcttc
    28561 cagttgtacc gcatggaacg gaccgtcaca tacagtccgc ggagccggaa gaaccaccgg
    28621 ggtaccctgc tgcaatattc gtcaaaggct tcaccgaatt tgttcttcaa aaaatgttct
    28681 tcggcgagca caatgcactg gtaaataaat aaaaacaggg gcaaaacgat cagggtgtaa
    28741 taaagtgagt ttgccaatat acccaccccg gccagcatga gaatattgcc tacataaagg
    28801 gggttgcggc aatgggcgaa aatgccttcg gtcaccaggc cttcggcata gaccttcccg
    28861 tctttacctc cccgtacgat ataagccagg ccaatggtga gggcgcggat aaactgacct
    28921 ttgaaggtga tgatcagccc aaagacaatg ggccaccaga aatagccgcg gccaaaccgg
    28981 ttctcgctga agaggggtgg ggaagggata aacaaagcga ggtagaggac aataaagagc
    29041 aggttgcggt atttgaagaa aaaattgccg atggagatca tggtaggatt aataggattg
    29101 gtaggattaa taggattggt aggattaata ggattggtag gattaatagg att
//
LOCUS       DAOQXT010000057        28880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1898_length_28880_cov_7.303417, whole genome shotgun sequence.
ACCESSION   DAOQXT010000057 DAOQXT010000000
VERSION     DAOQXT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 28880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28880
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1898_length_28880_cov_7.303417"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKM27_10980"
     CDS             complement(<1..196)
                     /locus_tag="PKM27_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07828.1"
                     /translation="MIVTITLNPSIDISVIVPQLIPTEKLRCESYEKEVGGGGINVAK
                     GLHRLDVKTKALFFSGGHNGK"
     gene            240..893
                     /locus_tag="PKM27_10985"
     CDS             240..893
                     /locus_tag="PKM27_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNR07829.1"
                     /translation="MSIFSLLFIGLQLIVLPVLSFQSRSTLEESEEIPPRQALYLQSI
                     IMLTAIGGLAALVDWRETLEIRWFSPFTGPALALAGGIYLAGLLVNFINYRMSKEKED
                     KSAQMLPETKKEYLLWIFLCFTAAWAEEYIYRGVLTHLLSQNGMVFILAALLSAVSFS
                     FSHFTQGWLAIPVTFVFALGFQFLYAVSGSLALPFLVHFFYNISAEWVRKWLIRPVK"
     gene            complement(874..1653)
                     /gene="rsmA"
                     /locus_tag="PKM27_10990"
     CDS             complement(874..1653)
                     /gene="rsmA"
                     /locus_tag="PKM27_10990"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNR07830.1"
                     /translation="MIRAKKSFGQHFLTNESIARSIVDALAGFEPIGHLLEIGPGRGM
                     LSKYLAEKYQGLALVEADLDMVRLLAADPRYAACRLIHSDFLKLDLNNLFGGEPLVII
                     GNFPYNISSQIVIKMIDHRDLVPGMVGMFQKEMAQRIAHKPGSREYGVISVLTQAYYE
                     VDYLFTVNEGNFSPPPKVKSAVLRFTRKPEFHLDCDERLFRTVVKSCFLQKRKMIRNS
                     LKTRLSPDILADSFFDLRPEQLSVQDYVGIVKKIEAISPAG"
     gene            1732..2811
                     /gene="pdxA"
                     /locus_tag="PKM27_10995"
     CDS             1732..2811
                     /gene="pdxA"
                     /locus_tag="PKM27_10995"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNR07831.1"
                     /translation="MGSNGSNLIKIAITMGDFNGIGPEIIIKTLRHPKILEYCIPVVY
                     GSGKVLSYYKNVVKGDEFMFYQARSNEKLAPGKVYVVNCWQENVTITPGKVTEAGGKY
                     ALMAIDQAIADIRSGWIDGLVTAPIHKKSMQLAGFAHAGHTEYLARQLHAQDYLMMMI
                     GQQMRMGLLSTHIPLREVSAMVTLARIKESIRILHQTLQKDFGVDRPLIAVLGLNPHA
                     GDEGVIGDEEINTIIPGIQELRNNGLSLFGPFSSDGFFGSGQFKKFDGVLAMYHDQGL
                     IPFKSFEFTTGVNFTAGLPVVRTSPDHGTAFDIAGQNIADPTSFREALFGAIDISRQR
                     KKYQEDRANPLNKHQKISEEKEAES"
     gene            complement(2812..3345)
                     /locus_tag="PKM27_11000"
     CDS             complement(2812..3345)
                     /locus_tag="PKM27_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNR07832.1"
                     /translation="MEWFYIERFAPLAVEEMHRVGIPASIKLAQAVIESNSGRSDLAI
                     QSNNHFGIKCKSWWKGQSYYHKDDDLDARGRLMESCFRAYADIESSFRDHSDFLKSSP
                     NYIPLFKLDPTDYKGWATGLKSCGYATDKSYADKLIRTVETFQLYRYDTYKATIYDLP
                     ADKGRSPSSLPRETTVD"
     gene            complement(3451..4182)
                     /locus_tag="PKM27_11005"
     CDS             complement(3451..4182)
                     /locus_tag="PKM27_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR07833.1"
                     /translation="MIRTIVIEDEPLARNLLTQYIEKVSYLELVHTFSDPLKGLEFVN
                     NHEVDLMFLDIQMPNLSGIGLLRILQKKPEVILTTAYSDYALEGYDLNVTDYLLKPIT
                     FERFLQAVEKVKQRKENARQNNDPQIPPTSPPADDFMFVKDGTKLIRVNVNNIMYIEG
                     LKDYVTIYTPEQKIVSLQRMKNLENQLPKSQFIRIHHSYIVSLKWIHSIHKDYVEINK
                     VQIPIGETYRKAFREFIDQQKFLGD"
     gene            complement(4183..5232)
                     /locus_tag="PKM27_11010"
     CDS             complement(4183..5232)
                     /locus_tag="PKM27_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNR07834.1"
                     /translation="MVQTFLQKHRMLTLHTLFWCSYFLLFFYQISGPRGNEQPRAFAE
                     VFLNASTHTLFLMSVAYVNYFLFLPRFLSHKNGLRYALEFLVGAVLILIVYVILKRWI
                     ADGYTHKAMFYYNHRFPISSFVTSVLTASFVAVLRFFTEWKDLEEQRKELQNEKLSAE
                     LKFLRSQVNPHFLFNTLNNLYSLAYSMSPQTTEVISRLSQMMRYMVYDSNHDRVALER
                     EVDYMQNYISLEKLRLNDQIPIRFEVEGDLGQKKIAPLLLIPFLENAFKHGVSNTNPD
                     SWVKANLIVNANQLSFEVANSISVQAGTAGSAQSGIGLENVKKRLELNYPDRHKLKIS
                     QDNKQFTAKLDIQLA"
     gene            5452..8226
                     /locus_tag="PKM27_11015"
     CDS             5452..8226
                     /locus_tag="PKM27_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR07835.1"
                     /translation="MQKFKALCSLILAAFSLGPMLMAQNTGIPDGKNPSQIKGEGVIQ
                     GLLLDSITKKPVEFATIALIKKQTEATVDGTITDENGEFELKHIPDGEYFLEYGFLGY
                     ETKRSALITMVKDSKLKMGKIYLSPEALVLNTVTVEGTTEIIEEKVDRLVYNAEKDLS
                     AKGGDASDVMRNVPLLTVDLDGNVSLRGSSNVRVLINNKPSSIMAGSVADALKQIPAD
                     MIKSIEVITSPSARYESEGSVGIINIITKKNTLQGMTLGIDLSVGNRGSMLGLNGNFR
                     RGNMGFSLRGHGRAQYNVKGAFENTQTTLTSLGLQSSTQSAETITNMLHGNYNLGWDW
                     DITKKSNLSAGLRFGTRSGLSQQDNLITRTLLPGSTTPILSGRNVDSKDLSFSYDLNV
                     AFTHSVRPQEEFSFLGLYSRNNRTNDFEADLLNNVDFETITARQKNDNVSFNEEKTIQ
                     AEYQAPLGERQLIEFGGKGIFRLVNSDYQYFLSDGPNSPYYLDENRPSNVLNYDQTVG
                     AGFLSYTLSTRNKFTFKIGSRLEYTDINARFQENNGETAAIPSYWSLIPSANISKNIS
                     KTLTLKAGYNRRIQRPGIQILNPNINLANITNVSVGNPYLDPELTDNYELTASINMKG
                     VYLTAATFYRHTGNAITSLRDTTSVLNPNPDEPGYISAIRTTYQNIGKENTVGFNVFG
                     NVTLVKNWTINGGFDIYNVNLTNNNPDPAYNASNNGWVLGGRMFTNYKFKNGWGVQGG
                     GGARGRQVNLQGYSGSFAFYNLGLRKEFKNGKGNLNIAAENFLNFPFRVNSSSKTALL
                     SQSNTNSFYNAGIRIGVNYRIGKMTFEERRRKSINNDDIKNDGGGNDQGMGGGGNEQP
                     QNQGARGQNPGQNRNAANNQAAGQKPQPAPNQAKSTSTGKPQLVPANTEKKEAPKEAV
                     PFKKPENKNQ"
     gene            8807..10198
                     /locus_tag="PKM27_11020"
     CDS             8807..10198
                     /locus_tag="PKM27_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR07836.1"
                     /translation="MKTGPSPLPSDEFFENLRRKEYARLDETRSIYLDYTGGNLFPLS
                     LLEDHYQTLKTKVMGNPHSTNPSSLLSTQMVASARQKVLEYFNAEDYYCIFTTNASEA
                     LHIVGENYDFDPESFFLLTSDNHNSVNGIREYCHHKGGRHEYFSVNYEDLCINEIALK
                     TRVEQYQGFKRKLFAYPAQSNVSGVRHSLELIPWMKDKGWDVLLDAAAYVPTSRLDLK
                     LYQPDFVCLSFYKIFGYPTGIGCLLVKKDSFHKLHKQWFAGGTVTLVSVNYDRYYLAP
                     DHERFENGTINYLGIPALEKGLNFIENIGIDRIHQRIEDLTGYLIRRLGDLKHDNGRP
                     LLRIFGPEDIKDRGGNIVLNFFDPEGKPWPFELIEKEANARNISVRTGCFCNPGIDEI
                     NHCLSSDALAPYFSSRDQGNYQDMVQFLGTMRGSVRLSVGIPTVRKDIDGFVEFAKQY
                     LGLEAPSQVMQQF"
     gene            10361..11440
                     /locus_tag="PKM27_11025"
     CDS             10361..11440
                     /locus_tag="PKM27_11025"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR07837.1"
                     /translation="MNKLPLFSFLFVFLFRLAPALLAQAPDDPVDRCIKKFMAEKSIP
                     GLAVAILRDGIPIKVKGYGWADLEHQVSVHPETIFQSGSIGKQFTAAAILLLKREGKL
                     NLEDEISRYFNGSPATWTGIKVRHLLTHTSGIPDWEYDSTVIRLNTDYTEQELLEKAM
                     RLPPRFKPGEKWEYCNTGYVLLGILIRQLTGRFYGDYLREKVFAPLSMETARVIDEQE
                     IIKNRASMYILKEGTVKNQSWVSPTLNTTADGSIYLTILDLLKWDMALSKEKLLDRED
                     LLVMFSPVKLNDGSLYPYGFAWFLDPVNRHRAYQHSGSWQGFNSYIAKYPDDRLTVMV
                     LTNLNPSNPGQLTREISALYVPDLN"
     gene            11628..13202
                     /locus_tag="PKM27_11030"
     CDS             11628..13202
                     /locus_tag="PKM27_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNR07838.1"
                     /translation="MPDPLQSRRTFAIISHPDAGKTTLTEKLLLFGGAIQVAGAVKSN
                     KIKKHATSDFMEIEKQRGISVATSVMAFEYKGLLINLLDTPGHQDFAEDTYRTLTAVD
                     SAVVVIDVAKGVETQTEKLVEVCRMRQTPMMVFINKLDREGKDAFDLLDEVEQKLKLK
                     VTPLSWPVGMGQRFQGVYNLFEKKLCLFKPHHKQGEDETLVIEKLDSKDLETHIGTAA
                     AKTLREELHTLMTVYPPFEVKEYLDGHICPVFFGSAVNNFGVKELLDCFVQISPDPLP
                     RQTEERLVHPEEKKFSGFVFKIHANIDPRHRDRIAFVRVVSGKFERNKPYHLVRQDKD
                     LKFSNPYSFLAAKKEVVDEAFPGDIVGLYDSGNFKIGDSITEGEDLHFKGIPAFSPEQ
                     FRYVINNNPLKTKQLNKGIEQLMDEGVAQLFIKEDNGRKIIGTVGALQFDVIQYRLEH
                     EYGASCSYEPLSLHKACWVKSPDAAALKEFITRRKKDLALDKDGKLVYLAESPWALRM
                     VQENHPKIEFRFKSEE"
     gene            complement(13232..13957)
                     /locus_tag="PKM27_11035"
     CDS             complement(13232..13957)
                     /locus_tag="PKM27_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07839.1"
                     /translation="MNRETKFIQIQGVQREYLLCLPNQAPAGILLVFHGNGGTAEGFS
                     NKYHLEQAFTDFIVIYVQGIPGIGGGFDPKGLKNGWQRKVGEGEDRDLRLMDVLVPEL
                     LREWPFLKDRVFAFGHSNGGRFVYLLWNQRPDLFRGFIINAHQGTDLLNTAQPKNAMV
                     LTGAKDRVVNNANQMKSAEMIRTRLETDREQKINDLLTIYSNPSTGLCLYHYLHPGGH
                     DLPKTAVPFIGKFLSAIVDIPNF"
     gene            complement(14389..15510)
                     /locus_tag="PKM27_11040"
     CDS             complement(14389..15510)
                     /locus_tag="PKM27_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07840.1"
                     /translation="MIKMWLIVRKELALVFRQPAILRMMILMPLMQLILLPLAADYEV
                     KNINLQVIDQDHSSYSRSLTQKLGASPYFKLVEANGDPAMARRNLEQNKADLTLNIPA
                     GFEKDLIRESETALSIEADAVNGNKASLGCAYASQIIGDFNREVREKLMQLPRFNDFP
                     VIEVRSSFWYNPFIKYPLFMVPGILALLVTMVGGLMAALNIVQEKEIGTIEQLNVSPL
                     RRYQFLMGKLIPFWFLGMVSVTLGLIVGYLVYGIVPRGSLLTIYFFSAVYLIGALGVG
                     LLLSTLVDNQMQATLFAFFIIMFFVLMGGLYTPIDSMPRWAQILSSFNPVTYFIRVLR
                     FIVIKGSGISDLLVELRSMLLFGIVLNVLAVMNYRKRSA"
     gene            complement(15507..16616)
                     /locus_tag="PKM27_11045"
     CDS             complement(15507..16616)
                     /locus_tag="PKM27_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07841.1"
                     /translation="MNSRFAAMTIKEFKHVLRDPRSLFFLLAQPVMLMLLFGFALSNE
                     VKESRVAIIDQARDEETKKLTDRLDQSEYFDVMGHYADPSVVPALFEKGAARLAIVFP
                     PHFANDLEHLNAGQIQVLADASDPNTAAIATSYASAIFGAYQDELLGLMKLPYKINIE
                     TRMLYNPQLVSAFSFVPGVMTLILTILCTMMTSVAIVREKELGTLEVLLASPMHPMQI
                     VVSKAVPYLVLAMIDVAVILLLAVYVLGVPIRGSLFLLMAENTLYISNTLALGLLVST
                     ITEKQQVAMFVSLVGFMLPALLFSGFMFPIENMPWPLQWISHAVPTRWYYRIVNAIMI
                     KGLGLASIWKETLILAVMTLGLLVLAVKKFKVRLL"
     gene            complement(16724..17482)
                     /locus_tag="PKM27_11050"
     CDS             complement(16724..17482)
                     /locus_tag="PKM27_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR07842.1"
                     /translation="MKAIEVKDLTRKFGDFTAVDHISFDVSKGEIFGFLGANGAGKTT
                     AMRMLCGLLKPSSGEGRVAGFDINLQSEEIKKNIGYMSQKFSLYNDLTARENILFYGG
                     LYDIPYKELKIKSERLLDRLGLGPYANKLLRDYPLGWKQKLAFSVAIQHQPMIVFLDE
                     PTGGVDPITRRQFWDMIYESAEGGLTVFVTTHYMDEAEYCHRVCIMVDGRIAAMDTPQ
                     SLKKKYESQNMDEVFRKLARGAVRSPDVPGRGKR"
     gene            complement(17479..18399)
                     /locus_tag="PKM27_11055"
     CDS             complement(17479..18399)
                     /locus_tag="PKM27_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR07843.1"
                     /translation="MEMKTIQTDRLTRTFDKGKVTALQEVSFAVEKGEIFGLIGPDGA
                     GKTTLIRILASLILPDSGSASLAGFDPVRDYARVRQIIGYMPGRFSLYQDLSVQENLR
                     FFATVFKADLETNYPLIKDLYDQLAPFKNRRAGQLSGGMKQKLALCCALVHEPEILLL
                     DEPTTGVDPVSRREFWDMLIRLKARGITILVSTPYMDEATLCDRIALIQKGTILETGT
                     PAEIPRRLSRPVYAASAGDMYLLLRAVRDYPETHSCYTFGESHHVQLKSGSPQDLLDF
                     LGGRIPGPVALDQIEPGIEDVFMDKMTSRT"
     gene            complement(18390..19400)
                     /locus_tag="PKM27_11060"
     CDS             complement(18390..19400)
                     /locus_tag="PKM27_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNR07844.1"
                     /translation="MAKGKFSPSRIRLLPGLILLLLVFTGACNNKKRDYQASGIFEGR
                     EVIVSAESTGKILQLDLEEGMVLDAGAPVGMISCSQQELAVQQARASLESLEDKKADA
                     GPNIKVLEQQAVLNQNQIRILEDQLAALVRDETRLQRLVDAEAAPVKQLDDVRTQKSV
                     VEKQIAVAKSQVDITKRQIEAARQNTAIQNRAILSEKSPLSAKLEQLEDLEEKCRITN
                     PMRGTVLTQYAYAHEMVNAGKALYKIANLDTLYLKAYVTGTQLGEIRLGQDLTVWIDQ
                     GGEAVLSYPGNISWISDKAEFTPKSIMTVDERANLVYPVKIKVVNDGRIKLGMFAEVQ
                     WK"
     gene            complement(19387..20670)
                     /locus_tag="PKM27_11065"
     CDS             complement(19387..20670)
                     /locus_tag="PKM27_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR07845.1"
                     /translation="MKHAHMLKRFVLALFLIQAVVLTGFSQNRVSLVDLYAAIEKNHP
                     YQRMAAIAKSTEENQKKINALSRIPQASFLGQASWQSDVTEIPVVLPGLDIPGQPRDQ
                     YRALLELSQLIYDGGQRKAQDEQSVRAYDMDLAGTQVMIHPLKELVCKTFFSVLLADL
                     SIRQWSLLAEDLEKKAARLNRQIGEGMAAGSQEAALQIKIRETNQAMLEIKKQKSNAL
                     SSLELLTGMSLDSSMQFVPSLSPEDLAGTRPELALFEAQRKFQAASFSLSQTRFQPKV
                     QLFGQLGYGRPGLNLLSDEFKPYTILGLKWQWNLNNLYSRQGQREKDLLQAGLDKIKV
                     QEESFNLQEKIRIRAQETEIEHLGRLLLEDEAVIALHERVLKSSEAQLETGVATVNEY
                     ATDANNLTQARIKKDMHQIQLLQSKELLNLIHGKR"
     gene            complement(20683..21294)
                     /locus_tag="PKM27_11070"
     CDS             complement(20683..21294)
                     /locus_tag="PKM27_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR07846.1"
                     /translation="MSVVKKNIEEMILASARRHFSRKGFAGASLQEIAKEAGTTKSMV
                     NYYYRSKEKLFDSIFLSEFRNLFSSIAGVLLEEAPLEKKLEQIVSLDIDKLLGMPGLP
                     VFILSELHRNPEMIFKGMENIPVNRVLTKLEKQIKEESLRGIIRYISARELILNIQAM
                     TIFPFLAKPILTGKLGFTEKSFNSLMQKKKKEVVTMIWNSIKK"
     gene            complement(21479..22324)
                     /locus_tag="PKM27_11075"
     CDS             complement(21479..22324)
                     /locus_tag="PKM27_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07847.1"
                     /translation="MKISIILCTIGFLAPLLASSQKMAEFESDFTMTNAQGKAVKFTI
                     GFDRNAYEEFGDTIFGEYDLYRPNQSILFRDSFDIILHPLRVYSFNRSIIHSPKCGEY
                     EYGPLQLIVKVNMYMFPLTLKWNKTDFNGICVARSCFAPSIIGPLTGDYSDILLLRDQ
                     DSLIISHEMAKSLSLHYNLNSSDTIRYMFFLFRPRGASTSEHGNPINPKVIYNKGRFI
                     LSQVEPFDKMDVYSLDGRLLNRVRLPSTYPGEITIDWKPPGAGVYIVSLTGSRSLKPF
                     KLYCD"
     gene            complement(22478..24496)
                     /locus_tag="PKM27_11080"
     CDS             complement(22478..24496)
                     /locus_tag="PKM27_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR07848.1"
                     /translation="MRRSIVGCLIFIQTALGAQAQQSMSDTILEVKEVVVTASRMAQA
                     RASVNMPVQRIEREKLNEVASRNLVEALQHEGIWMQKTNQGGGSPFIRGLTGNQVLIV
                     KDGIRLNNSIYRYGPNQYLSLQSLFEPGQIEVIRGSGAVQYGSDAIGGVIHLISSNPV
                     TGAGQTFSGNVYTQWASSGVEKLANAGIRFSKNKFGLSMRGALAGYGDLQGGKKTGKQ
                     IPSGYDEGSADLKARWENPAGAWDLSWSATRQTQVPVYHKVVLEGFQRNQSDQLSHHL
                     AFLRNKYEFGGSRFFKQIETTLSWQKLSEQRSLQKKTTDPVRKEEDIAGVWGFSSQLE
                     LSPSNNTSGILGVDLYRDKVNSTAGLETTVPGSPALRGLYPDGATYMNAGFFNDWNFD
                     WESWDLALGWRYNIYQLKTNEASLGEVVLKPSALVWHLALGRTMGAHRIFIHADKTFR
                     APNIDDAGTLGIVDFRYEVPNYELSPEKGLHLGAGHRWVSKSLQLETSLFYLKLHDLI
                     TRTAISGDSIQGYPVFIKKNADRSYIYGLESSLNLKFSSRWTVRGMIQYSFGQNQSAD
                     EPIRRIPPLFCIGKIQYQPGLRWSFYAESLNAGRQDRLSAGDISDNRISRTGTPAWNI
                     LNLGLTVKLKSLECRAGVENLFNQDYRVHGSGINGAGRLLRLGIGVSW"
     gene            complement(24506..27259)
                     /locus_tag="PKM27_11085"
     CDS             complement(24506..27259)
                     /locus_tag="PKM27_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HNR07849.1"
                     /translation="MKPFIYIGLLLLFLCFACRNKSADQIWVSSAPAGNEYCFINERE
                     AILPNGRYVRPMGKTVRIAPHPYGLVLSADGRLAVTANSGTNPFSITVLRNPFTDSMT
                     TLQIPKSANTDDDLLSAVFMGLAISPDDKTIYVAGGQTTKIFAFDALSGEKKSTISCT
                     SDQDGFRYNDGYIGDMIMTRDGKTLYAVDQTGFRVIEVNLETGNVVRNWRTGRYPFGI
                     CLSPDEKKMYVANVGMFEYSLVNNMDSNSVGLRPLDFPAFAYGSEEMVKGIDRDSIQV
                     KGLGEMNADEAFSIWVFDLESPQSAPKARIKTGFRVGEKLDGIPAVGGSSPNSIVTDG
                     QYVYVSNGSHDCISVIDAARDSLVKSIDLTLDDRLGPMKGIIPFGLSLDPERKKLYVA
                     ESGINAVGVIDLNTLSLQGHLPVGWFPSKLRVSPDGKKLLVANAKGFGSGPNGGPSYT
                     SGPEGDYIGSLMKGSVTILDIPSEAELSKFTGEVIANNFSWKPLDKKSMGPVPPHPAS
                     GIPSPIKHIVFVSKENRTYDEVFGQIKNGKGEPSMARYGHRVSFYNKKRTDSVINTTV
                     MPNHLALAQRFAISDNFYVDSDHSADGHRWLAGTYPNEWLETHTSAAYGGKRDLNHRS
                     NAPGRFGMTGASGAIYPEEYNPHGSIWDHLHRNGKEFFNFGFGVEFDAGSFADPSMKY
                     GGVKYLVNYPLPGPLYDRTSRRYATYNMAIPDQFRLDVFIEEFNEKWGDGKSLPQVIT
                     IILPNDHGAGDRPEAGFPFRESYMSDNDLALGRLVEFLSHTPYWKEMAIVITEDDSQD
                     GRDHIDAHRSILMVVSPYAKKNYVSHTHYSFGSIFKTFWNILGIPYLNQYDAGATDLA
                     ECFTTQPDFSPYQATMVDTLVFNPRKALTPLNEEFDWKALGTGPKLDDETFIRTSIQE
                     YLK"
     gene            complement(27415..28323)
                     /locus_tag="PKM27_11090"
     CDS             complement(27415..28323)
                     /locus_tag="PKM27_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNR07850.1"
                     /translation="MKRRKFIQSAALAVPAGIAGMPNLSGNLKETSAPAFKMDYAPHF
                     GMFKNSAGEDLVDQLKFMADQGFRSLEDNGMLARPKEVQDKIGSTLTALGMRMGVFVI
                     EGGDNWKISLATGKKEFLDKFVATCKASVELAKRVNAKWMTVVPGYFERNLPLDIQTA
                     NVIEAMRRGAEVLEPHGLTMVMEPLSDNPDLFLRHAAQTYLICKAVNSPACKILYDIY
                     HMQRNEGQLIPTIEKCWDEIAYFQIGDNPGRKEPNTGEINYKNIFKYIHGRGYTGVMG
                     MEHGNAFPGKEGEMKLIQAYREVDIS"
BASE COUNT         7193 a         7440 c         7187 g         7060 t
ORIGIN      
        1 acttcccatt gtgcccaccg gagaaaaaca gggcctttgt tttgacatcc aaacggtgca
       61 atcctttggc gacattgata cctcctcccc ccacttcctt ttcataactt tcacaccgca
      121 gtttctcggt tggaatcaac tggggcacga tcaccgagat atcaatggac gggttcagtg
      181 taatggttac aatcatactt ggcgtgtaaa gattgcatat tttaggctat ttttgaccgg
      241 tgtcgatatt ttctttatta ttcattggcc tccagttgat tgtactccct gtactcagtt
      301 ttcaatccag gtcaaccctt gaagaatcag aagaaattcc accccggcag gccttatatc
      361 ttcaatccat tattatgctc accgcgatcg ggggcctggc tgcactggtg gactggaggg
      421 aaaccctgga aatccgctgg ttcagcccgt tcaccggccc tgccctggcc ctggcagggg
      481 ggatatacct ggccggcctg ctggtcaact ttatcaatta ccgcatgtcc aaagaaaagg
      541 aagacaaaag cgcccagatg ctgccggaaa ccaaaaagga atacctgctt tggatttttc
      601 tttgctttac cgcagcctgg gccgaggagt atatctaccg tggggtcctg acccacctcc
      661 tgagccagaa tggcatggta tttatacttg cagccctgct tagcgccgtc agcttttcct
      721 tttcacactt tacccagggt tggctggcca taccggtgac cttcgtattt gccctgggtt
      781 tccagtttct gtacgcggtc agcgggagcc tggcccttcc tttcctggtc catttctttt
      841 ataatatttc ggcggaatgg gtgcggaaat ggcttatccg gccggtgaaa tagcctctat
      901 tttttttacg atgccgacat agtcctgcac cgataactgc tccgggcgca ggtcaaaaaa
      961 agagtcggca aggatatcag gggacaaccg ggttttgagg gaattccgga tcatttttct
     1021 tttttgaaga aaacaggatt tgaccaccgt cctgaaaagg cgttcatcgc agtccaggtg
     1081 aaattccggt ttccgggtaa accggagcac ggccgatttt actttgggag gcgggctgaa
     1141 attgccctcg tttaccgtaa acaggtagtc cacttcgtaa taagcctggg tcagtacgct
     1201 gatcaccccg tattcgcggc tgccgggctt atgggcaatg cgctgggcca tttctttctg
     1261 gaacattccg accatacccg ggaccaggtc gcggtgatcg atcattttga tgacgatctg
     1321 ggaggatata ttgtagggga aattgccaat gatcaccagg ggttctccac cgaagaggtt
     1381 gttgaggtcc agcttcagga aatcggaatg gatcagccgg catgctgcgt accgggggtc
     1441 cgcagccaac aaccggacca tatccaggtc tgcctcgacc aaagcaaggc cttgatattt
     1501 ttcagccagg tatttactaa gcatgccccg gccggggccg atttcgagga ggtggccgat
     1561 gggttcaaaa ccggccaggg cgtccacgat ggaccgggca atggattcat tggtaagaaa
     1621 atgctggcca aaggacttct ttgcccgtat catactgcaa tgataccatt tctaaccctg
     1681 accaaacccc ctgatttata ttatcttcgg cccaaattag cgttaaataa aatggggagt
     1741 aacggatcaa acctgatcaa gatagccatc acgatgggtg atttcaacgg aatcggtccc
     1801 gagatcatca ttaagaccct gcgccatccc aaaatcctgg aatactgtat cccggtggtg
     1861 tatggttccg gcaaagtgct ctcctattat aaaaacgtgg tgaaaggcga tgaatttatg
     1921 ttttaccagg ccaggagcaa tgaaaaactg gcacccggaa aagtttacgt ggtcaactgc
     1981 tggcaggaaa atgtaaccat cactccggga aaggtgaccg aagccggtgg aaaatacgcg
     2041 ctgatggcca tagaccaggc cattgcggat attcgaagcg gctggatcga cggcctggtc
     2101 acggcaccca ttcataaaaa atccatgcag ttggcgggct tcgcccatgc cggccatacc
     2161 gaatacctgg cccgtcagct ccacgcccag gactacctga tgatgatgat cggccagcag
     2221 atgaggatgg gcctgttgtc cacccatatt ccgctcaggg aggtgtccgc gatggtcacc
     2281 ttggcccgca tcaaggaaag catccggatc ttgcaccaga cccttcaaaa agactttggt
     2341 gtggaccggc ctttgatcgc cgtactgggc ttgaaccctc atgccgggga tgaaggcgtg
     2401 atcggggatg aggagatcaa caccataata cccgggatcc aggaactcag aaataacggc
     2461 ttatccctct ttgggccctt ttcctccgat ggttttttcg gatccggtca gtttaagaaa
     2521 ttcgacgggg tgttggccat gtatcacgat cagggcctga tcccgttcaa gtcctttgag
     2581 tttacaacag gagtgaattt caccgccggt ttgccggtag tgaggacttc ccctgaccat
     2641 gggaccgctt tcgatattgc cggtcagaac attgccgatc ctacctcctt ccgtgaggca
     2701 ctgttcgggg ccatcgatat ctcgcgtcaa aggaaaaaat accaggagga ccgtgccaat
     2761 cccctcaata aacatcaaaa gatctccgaa gaaaaagagg ctgaatcctg atcagtccac
     2821 cgtcgtttcc ctcgggagcg aggaggggga cctgccttta tcggccggga ggtcataaat
     2881 ggttgcctta taggtgtcgt accggtacaa ttgaaaagtt tcgaccgtcc ggatcagttt
     2941 gtcagcatag gatttatccg ttgcataccc acaggatttg agccccgtgg cccaaccctt
     3001 gtaatccgtg gggtccagtt tgaagagcgg aatatagttg ggagaggatt ttaaaaaatc
     3061 ggaatggtcc ctgaaggaag attcgatatc ggcataggcc ctgaaacagg attccatcaa
     3121 tctgccgcgg gcgtccaggt catcgtcctt atgataatag ctttggccct tccaccagga
     3181 tttgcatttg atgccaaaat ggttgttgct ttggatcgcc aggtcgctcc ggccggaatt
     3241 ggattcgatc acggcctggg cgagtttgat ggaggcgggt ataccgacgc ggtgcatttc
     3301 ctccacggcg agcggggcaa accgctcaat gtagaaccac tccacttttc tggaatcttc
     3361 cggaccggaa aacgatgaac acaaaaccca aatcaatact gcaagaaccc cagacttcat
     3421 agccctgaat ccagtcaaaa actgtgccag ttagtccccc aggaattttt gctggtcaat
     3481 gaattcccgg aaggctttgc ggtaggtttc cccgataggg atctgcactt tgttgatctc
     3541 cacatagtcc ttatggatcg agtggatcca cttcagggaa acgatatagg aatggtggat
     3601 gcggataaac tgggatttag gcaactggtt ttccaggttt ttcatccgct gcagggatac
     3661 tattttctgc tccggggtat agatggtcac ataatccttg agcccttcga tgtacatgat
     3721 gttgttgacg tttacgcgga tcagtttggt cccgtccttg acaaacataa aatcgtctgc
     3781 gggcgggctg gtcggaggga tttggggatc gttgttctgg cgggcgtttt ccttccgttg
     3841 tttgactttt tcaaccgcct ggaggaagcg ttcaaaagta atgggtttga gcaggtagtc
     3901 cgtgacgttc aggtcgtatc cttccagggc ataatcggaa taagcggtag tcaggatcac
     3961 ttccggtttt ttttgaagga tgcgcagcaa cccgatcccg gaaaggttgg gcatctggat
     4021 gtccaggaac atcaggtcga cctcatggtt gttgacgaat tcgaggccct tcagcggatc
     4081 tgaaaaagta tggaccagct ccaggtagga cactttttcg atatattggg tcagcagatt
     4141 tcgggctagc ggttcgtcct caatgacaat cgttcggatc atttaagcca attgtatatc
     4201 caatttagct gtaaactgtt tattatcctg ggatatttta agcttgtggc ggtcgggata
     4261 attcaattcc agcctttttt tcacattttc aaggccgatc ccggactggg ccgaaccggc
     4321 tgtgccggct tgaacggaaa tgctgttggc cacctcgaaa ctcagttggt ttgcattgac
     4381 gatcagattg gctttcaccc agctgtccgg attggtattg gaaacaccgt gtttgaacgc
     4441 gttctccagg aaaggaatca agaggagggg ggcaattttt ttctggccaa ggtctccttc
     4501 cacctcaaac cggatgggaa tctggtcgtt gagcctgagt ttttcaaggc tgatgtaatt
     4561 ctgcatatag tccacttccc gctccagggc cacccggtcg tgattggaat cgtacaccat
     4621 atacctcatc atctgggaaa gcctggaaat gacttcggtg gtctgcgggg acatgctgta
     4681 tgccagggaa tacaaattgt tgagggtatt gaataaaaaa tgagggttga cctggctccg
     4741 taaaaacttc agttccgccg ataatttttc attctgcaat tccttgcgct gctcttcaag
     4801 gtccttccat tcggtaaaaa accggagcac ggccacaaaa ctcgcagtaa gcacggaagt
     4861 cacgaaagaa ctgatgggaa agcggtgatt gtaataaaac atggccttat gggtgtaccc
     4921 gtcggctatc cagcgcttga ggatgacgta gacgatcagg atgagcacgg cccccaccaa
     4981 aaattccagg gcgtagcgca gcccgttttt atgacttaaa aaccggggca gaaaaaggaa
     5041 gtaattgaca taggccacgc tcatcaggaa gagggtatgg gtactcgcgt tcaggaacac
     5101 ttcggcaaag gcccggggct gctcgttccc ccgcggaccg ctgatctgat aaaaaaacaa
     5161 taagaaataa ctgcaccaga acaaggtgtg cagcgtgagc atgcggtgtt tctgcaggaa
     5221 ggtttgaacc atgttacaat gcaaaattac cctagaagaa aggcattccc gttaactccg
     5281 ttcatttaca agataaaata gtatcaaatt tcgactgccc catatttttg atagatgcac
     5341 cggtcgccgg gaggggcaag aggaacagac gaataaagtc cccggatggt cgattgatat
     5401 ttgacaggct ccccggtccc cctctacctt tgggtcaaca atccataaaa catgcagaaa
     5461 tttaaagctt tgtgttccct gatcctggcc gcttttagtc tcggtcccat gctcatggca
     5521 caaaacaccg gcatcccgga cggcaaaaac ccctcccaga taaaagggga aggcgtcatc
     5581 cagggtttac tcctggacag catcaccaaa aaacccgtgg aatttgctac gatcgccctg
     5641 atcaaaaaac aaaccgaggc gacggtggac ggaacgatta ccgacgaaaa cggcgaattt
     5701 gaactcaaac acattcccga cggcgaatat ttcctggaat acggttttct cggctatgag
     5761 accaaaagaa gcgcactgat caccatggtt aaagattcca aattaaaaat gggaaaaatc
     5821 tatctttccc cggaagccct ggtgctgaat accgtaaccg ttgaaggaac tacggaaatc
     5881 atagaagaaa aagtggaccg tctcgtctat aatgcggaaa aagacctttc cgccaagggc
     5941 ggagatgctt cggacgtgat gcgcaatgtg cccctgctca ccgtggacct cgatggcaac
     6001 gtgagcctcc gcggcagcag caatgtccgt gttctgatca acaacaaacc gtccagcatc
     6061 atggccggca gcgtggccga tgcgcttaaa caaatcccgg cagacatgat caagtccatc
     6121 gaagtgatca ccagcccttc cgcccggtat gaatccgaag gctccgtggg catcatcaat
     6181 atcatcacca agaaaaacac cctccagggc atgaccctgg gcattgatct ttcggtcggt
     6241 aacaggggtt ccatgctcgg cctcaacggc aactttcgca ggggcaatat gggtttcagc
     6301 ctgagggggc acggacgcgc ccaatacaat gtaaaaggcg ccttcgaaaa tacccagacc
     6361 accctgacca gccttggcct tcagtccagc acccagagtg ccgaaacgat caccaatatg
     6421 cttcacggaa attacaatct gggctgggac tgggatatca ccaagaaatc caatttatcc
     6481 gccggccttc gctttggtac ccggagcggt cttagccaac aggacaacct gatcaccaga
     6541 accctgctgc ccggaagcac gactccgata ttgagcggtc gcaatgtgga cagcaaagac
     6601 ctgagttttt cctacgacct caatgtggcg tttacccatt ctgtccggcc gcaggaagag
     6661 ttcagtttcc ttgggctgta ctccaggaac aaccggacca atgattttga agccgacctt
     6721 ttgaacaatg tcgatttcga aaccatcacc gcgcgccaga aaaatgacaa cgtcagtttt
     6781 aatgaagaaa aaaccataca agccgaatac caggcccccc tgggcgagcg acagttgatt
     6841 gaattcggtg gcaaggggat tttccgcctg gtcaacagcg attaccaata ttttttaagc
     6901 gatggcccaa acagccccta ctacctggat gaaaacaggc catccaacgt attgaattat
     6961 gaccagacgg ttggcgccgg ctttttatct tataccctga gtacccggaa taaattcacg
     7021 tttaagatcg gaagccgcct tgaatacacc gatatcaacg cgcgtttcca ggaaaacaac
     7081 ggtgaaaccg ccgccatccc ttcctattgg agcctgattc caagcgccaa tatatccaaa
     7141 aacatttcca aaaccctgac cctgaaagcc ggatacaacc gcaggatcca gagaccgggt
     7201 atccagatcc tgaaccccaa catcaacctg gccaacatca ccaatgtctc cgtcggaaac
     7261 ccttacctcg acccggaact cacggacaat tatgaactga ccgccagcat caatatgaaa
     7321 ggagtttacc ttactgcggc caccttctac cgccataccg gcaacgccat caccagcctc
     7381 cgggatacca cttcggtact caatcccaat cccgacgaac ccggatatat ttccgcgatc
     7441 cgcaccacct accagaatat cggtaaagaa aacacggtgg gctttaatgt ctttggtaac
     7501 gtcaccctgg tgaaaaactg gaccatcaac ggcggcttcg acatctataa tgtaaacttg
     7561 accaacaaca acccggatcc ggcctacaat gcctccaaca acggctgggt cttgggcgga
     7621 agaatgttca cgaactacaa attcaaaaat ggatggggcg ttcagggagg cggtggcgcc
     7681 cgtggccgcc aggtcaacct ccagggttat tccggaagct ttgctttcta caacctgggc
     7741 ctgcgcaagg aattcaaaaa cggaaaaggc aatttgaata tcgccgcgga aaacttcctc
     7801 aacttccctt tcagggtgaa ttcgagttca aaaacggccc tgcttagcca gtccaatacc
     7861 aacagttttt ataacgccgg tatccggatc ggggtcaact accggattgg taagatgacc
     7921 ttcgaagaac ggaggagaaa atcgatcaac aacgacgaca tcaaaaacga cggtggaggc
     7981 aatgatcagg gcatgggcgg cggtggaaat gaacaacccc aaaaccaggg ggcccgcgga
     8041 caaaatcccg gtcaaaacag gaacgccgcc aacaaccagg ctgcaggaca aaaaccccaa
     8101 cccgctccaa accaggcaaa aagcacctcc accggaaaac cccagctcgt cccagcaaat
     8161 accgaaaaaa aagaagcacc gaaagaggcc gttcctttca agaaacccga gaataagaat
     8221 caataattta attgaatcga aagaagcggg gtccagcgat ggatcccgtt tttttttggg
     8281 ggtggggagg ttcacacggt gttaaggaga actcggagat ccatgcgtgc caaggttcgc
     8341 acggagttga ggagaacacg gagattcaat tttatcagtc ccccgatcca taaaaaaact
     8401 tcgtgctctc cgcgccctcc gtgcgtgcca aggttcgcac agagctaagg agaacacgga
     8461 gattcaattt tatcagcccc cccaactaaa aaaactccgt gctctccgtg ctctccgtgc
     8521 gtggcaaagt tcgcacggag ttaaagagaa cacggagatt atattttata aggcttctcc
     8581 atcctaacta aaaaactccg tgctctccgt gccctccgtg cgtgccaagg ttcgcacaga
     8641 gctaaggaga acacggagat tcaattttat cagccccccg atccataaaa aaactccgtg
     8701 ctctccgtgc cctccgtgcg atccaaaccc acacactctc cacgccctcc gtgtgaccca
     8761 taccgtgcac cactcttaac cggaagggcc ttaatttagc accatcatga agaccggccc
     8821 aagtcccctc ccctccgatg agtttttcga aaacctgcgc cgcaaggaat acgcccgcct
     8881 ggatgaaacc cggagcattt acctggatta caccgggggc aatctgttcc cgctcagcct
     8941 gctggaagat cactaccaaa ctctgaagac aaaggtgatg ggcaacccgc attcgaccaa
     9001 cccttcttcc ctcttatcca cccagatggt ggcatccgca aggcaaaaag tgctggaata
     9061 ttttaatgcg gaggactatt actgcatttt tacgaccaat gcatccgagg ctttgcatat
     9121 cgttggtgaa aattacgact tcgaccctga aagcttcttc ctccttactt ctgacaacca
     9181 caattcggtc aacggcatcc gggaatattg ccatcacaaa gggggccggc atgaatattt
     9241 ttcggtaaat tatgaagacc tgtgcatcaa tgaaatagcc ctgaaaacca gggttgaaca
     9301 ataccaggga ttcaaacgaa aattgtttgc ctatcccgcc cagtccaacg tatccggggt
     9361 caggcattct ttggaactga taccctggat gaaggataaa ggctgggatg tactgcttga
     9421 tgccgccgcc tatgtgccta cgtcccggct tgatctgaaa ttataccaac cggactttgt
     9481 ctgcctgtcc ttttataaaa tctttggtta tccgaccgga atcggttgcc tgctggtgaa
     9541 aaaagacagc ttccataaac tccacaaaca atggtttgcc ggtggcacgg tgaccctggt
     9601 ttcggtgaat tacgaccgct attatctggc ccccgaccat gaacgatttg aaaacgggac
     9661 gatcaattac ctcgggatcc cggcgcttga aaagggatta aattttatag aaaatatagg
     9721 catcgatcgc atccatcaac gtatagaaga tctgaccggt tacctgatcc ggcgcctggg
     9781 agacctgaaa cacgacaatg gccggccgct ccttcgaatt tttggaccgg aggatatcaa
     9841 ggaccgggga ggaaacatcg ttcttaattt tttcgacccg gagggcaaac cctggccttt
     9901 tgaactcatc gaaaaagagg ccaatgcccg gaatatttcc gtccgcacgg gttgtttctg
     9961 caatccgggc atcgatgaaa tcaaccattg cctgagcagc gatgccctgg ccccttattt
    10021 ttcaagccgg gaccagggca attaccagga tatggtccaa ttcctgggaa caatgcgggg
    10081 atccgtccgg ctgtcggtgg gtattcctac ggtcaggaag gatatcgatg gatttgttga
    10141 attcgcgaag cagtacctgg gcctggaggc cccaagccag gtgatgcaac agttttaaat
    10201 ccgtttgtac aagaatccag ttcatgaaac ctccaaaagt caatcgcccg tgacggatga
    10261 tgccctcata cccgtttcac tcaatccgcc agaattcata cgcatctttg caggctatgg
    10321 ataccaacca gggcaggaaa atcagaaata accgcataaa atgaataaac tacctctctt
    10381 ttcctttctt ttcgtattcc tttttaggct ggccccagca ttgctggccc aggctcctga
    10441 tgaccctgtg gaccgctgca taaagaaatt tatggcggaa aagtccatcc cgggattggc
    10501 ggtggccata ttgcgggatg gaattcccat aaaagtcaaa gggtatgggt gggctgattt
    10561 ggaacatcag gtttcggtgc accccgagac gatttttcag tccggatcca tcggaaaaca
    10621 atttactgcg gctgctattt tgctgttgaa acgggagggg aaattaaact tagaggatga
    10681 aatatccagg tattttaatg gttcacccgc cacctggacc ggtattaagg taaggcattt
    10741 gctgacccat acttccggga ttcccgactg ggaatacgac tcaacggtta ttcgtctgaa
    10801 tacggattac acggaacaag aactgctgga aaaagcgatg cgcctgcccc cgaggtttaa
    10861 acccggagaa aaatgggaat actgcaacac cggatatgtt ttgttgggaa tacttatccg
    10921 acaactcacg ggaaggtttt acggcgatta tctgcgcgag aaggtattcg ccccactttc
    10981 tatggaaacg gcaagggtca ttgatgaaca ggagattata aaaaaccggg cttccatgta
    11041 cattttaaaa gaggggaccg tgaaaaatca aagctgggtt tcaccaactt taaatactac
    11101 ggcggatggg tccatttacc tgaccattct tgatctgctg aaatgggata tggccctttc
    11161 gaaggagaaa ttgctggatc gcgaggatct cctggttatg ttttctccgg taaagctcaa
    11221 cgacggaagt ctgtatcctt atggttttgc ctggttttta gatcctgtaa acagacaccg
    11281 ggcttaccag cacagcggaa gttggcaggg ctttaacagc tacattgcaa aatacccgga
    11341 tgaccgcctg acagttatgg tattgaccaa tttaaatcct tcgaaccccg ggcagctgac
    11401 cagggaaatt tcagcccttt atgttccgga cctgaattaa agctggctgt tgaacccaat
    11461 ccatgcaatg gctaaagaaa aatcaaggga aaaccaggat ggaagacaag gaaggtaaat
    11521 ttttgtgcgg atgagcctat ttcatcgttg aaagggtgac caacagatca agattcaggg
    11581 cacacgccct ggccacgcgg gaattcctat ttttgcaccc ctgaattatg cctgatcctc
    11641 tgcaatcccg gcgcaccttc gccatcatca gccaccccga tgcggggaaa accaccctga
    11701 cggaaaaact cctgcttttc ggcggagcga tccaggtcgc aggggcggtc aaatccaata
    11761 agatcaaaaa acacgcgacc tccgacttta tggagatcga aaaacaaagg ggcatctcgg
    11821 tggccacttc cgtgatggcg ttcgaataca aaggcctgct catcaacctg ctcgacaccc
    11881 cgggccacca ggactttgcc gaagacacct accggaccct caccgccgtg gacagcgccg
    11941 tggtggtcat cgatgtggcc aaaggggtcg aaacccagac cgaaaaactg gtagaggtct
    12001 gccggatgcg ccaaacgccc atgatggtct ttatcaacaa actggaccgg gaaggaaaag
    12061 acgctttcga cctgctcgat gaagtggagc aaaaactgaa actcaaggtg accccgctga
    12121 gctggccggt aggcatgggc cagcggttcc agggggtcta taatttattt gagaaaaaac
    12181 tctgcctttt caaaccccat cacaaacagg gggaggacga aaccctggtg attgaaaaac
    12241 tggattcaaa ggatctggag acccatatcg gtacagcagc cgctaaaacc ctgagggaag
    12301 aactgcacac cctgatgacg gtttatcccc cgtttgaggt aaaggaatac ctggacggcc
    12361 atatttgccc ggtttttttc ggcagtgccg tgaataattt cggggtgaag gaattgctgg
    12421 attgtttcgt gcagatctcc cccgatcccc tgccccggca gaccgaggag cggctggtcc
    12481 atccggaaga aaaaaaattc tcgggttttg tgtttaagat ccatgccaac atcgatccga
    12541 gacacaggga ccgcatcgct tttgttcgcg tggtcagcgg gaaatttgag cgcaacaaac
    12601 cctatcacct ggttcgacag gacaaggatc ttaaattttc caatccttat tccttcctgg
    12661 ctgccaagaa ggaggttgtg gacgaagcct ttcccggcga tattgtgggc ctttacgaca
    12721 gcggcaattt taaaatcggg gattcgatca cggaaggcga ggaccttcac tttaaaggca
    12781 tccctgcctt ctccccggaa cagttccgct atgtgatcaa caacaatccc ctcaagacca
    12841 aacaactcaa caagggcatc gaacaactga tggacgaagg ggtggcccag ctcttcatca
    12901 aggaagacaa cgggcgtaaa atcatcggta cggtgggggc ccttcaattc gacgtgatcc
    12961 aataccggct cgaacacgaa tacggcgcca gttgcagcta tgaaccccta tccttgcaca
    13021 aagcctgctg ggtcaaaagc ccggatgccg cggcattgaa ggaattcatc acccgccgca
    13081 aaaaggacct cgccctggac aaggacggaa agctggttta cctggccgaa tccccctggg
    13141 ccctgagaat ggtccaggag aaccatccaa agattgaatt caggtttaag agcgaggagt
    13201 gattttaaca aaggggtaga tattccaaat attaaaaatt tggaatatcc acgatggcgg
    13261 aaagaaattt tccaataaac ggcaccgccg ttttcggcaa atcgtgcccg ccgggatgca
    13321 gataatggta taaacagagg ccggtcgagg gattggaata aatggtcaat agatcgttta
    13381 ttttttgttc ccggtccgtt tccaaccgcg ttcggatcat ctcggccgat ttcatttgat
    13441 tggcattgtt gactacccgg tccttcgcgc cggtgaggac catggcattt ttgggctggg
    13501 cggtgttaag caggtctgtg ccctggtggg cgttgatgat gaagccgcgg aagaggtcgg
    13561 gtctttggtt ccacaacaaa taaacaaatc gcccgccgtt gctgtgtccg aaagcaaaga
    13621 cccggtcttt caaaaaaggc cattccctga ggagctcagg caccaggacg tccatcaggc
    13681 gcaggtcgcg gtcctcccct tcccccacct tgcgttgcca tccgtttttt aatcctttgg
    13741 gatcaaaccc cccgcctatg ccggggatgc cctggacata aatcacgata aaatcggtga
    13801 aggcttgttc caggtggtat ttattcgaga agccttcggc cgtgcctcca tttccatgga
    13861 agaccaggag gatcccggca ggagcctgat tgggaaggca gaggaggtat tccctttgca
    13921 ccccttgaat ctggataaat tttgtttcac ggttcattaa actctttctc tgatttaaat
    13981 tacaaactaa tgaaaatttc atgattctga atactcccgc tattggtcag atttgcccaa
    14041 tgccggcgtt tggaaccacc caattcgccc tgaaggggca tcatttattt aacgagccaa
    14101 tcctgtttat caaacatgga atcgttaatg agtataaacg gtggttaaaa atgttgtgaa
    14161 tagtgacagg gtatataaat taatgatgcc ccttcagggc gatccaacca caccatttcc
    14221 cgagcatcgg gcgatatgcc cgatgctcgg gaaatggcgc cccttcaggg ccaactcatg
    14281 caacaccggt ttgattagaa ttatacaatt agtgcgcgat agtatcttga aaattccagc
    14341 gtgaatagtc aatcccacac aaatagtcag ccatctcaat ttagttatct aagccgacct
    14401 cttcctgtaa ttcatcaccg ccaatacatt gagcacgatt ccaaaaagaa gcatcgatct
    14461 caattctacc aacagatcgg agatgccgct gcccttgatc acgatgaagc gtaataccct
    14521 gatgaaatag gtgaccggat taaaggacga aaggatttgc gcccaccggg gcatggagtc
    14581 gatgggcgtg tacaagcccc ccatcagtac aaaaaacatg atgataaaaa aggcaaacag
    14641 ggtggcctgc atctggttgt ccactaaggt gctgagcagt aagcccactc ccaaagcgcc
    14701 gatcaggtag accgccgaaa agaaatagat ggtcagcagg gaccccctgg gaacgatccc
    14761 gtacacgagg taacccacga tcaggcccag cgtcacggaa accataccga ggaaccagaa
    14821 cgggatgagt ttccccatta aaaactggta ccgcctcagc gggctcacgt tcagctgttc
    14881 gatcgtgccg atttctttct cctgtacgat gttgagcgcg gccatgaggc ctccgaccat
    14941 ggtgaccagg agggccagga tgcccggcac cataaacaga gggtatttga taaacggatt
    15001 gtaccagaag gaactgcgca cttcaatcac aggaaaatcg ttgaaacgcg gaagctgcat
    15061 cagtttctcc ctgacttccc ggttgaaatc cccgatgatc tggctcgcgt aggcgcaacc
    15121 cagggatgct ttgttaccat tcaccgcatc ggcttcaatg gacagggcgg tttcgctctc
    15181 ccggatcagg tctttctcaa aacctgccgg aatgttcagg gtcaggtcgg ccttgttttg
    15241 ttccaggttg cgccgcgcca tggccggatc gccattcgcc tcgaccagtt taaaataggg
    15301 ggaggccccc aacttttgcg tcaggctgcg cgaataggaa gaatgatcct ggtctatgac
    15361 ctggagattg atattcttga cttcgtaatc cgcggccagg ggcaacagga tcagttgcat
    15421 gagcggcatc aggatcatca tccggaggat ggcaggctgc cggaaaacca gggctaactc
    15481 ctttcgaacg atcagccaca tttttatcat aggagccgga ctttaaattt ttttactgcg
    15541 aggaccagca gtcctaaggt catgaccgcc aggatcaggg tctctttcca gatgctggcc
    15601 aaaccgaggc ctttgatcat gatcgcattg acaatgcgat aataccaacg ggtgggaacc
    15661 gcatgactga tccattgtaa gggccagggc atgttttcaa tgggaaacat aaatccgctg
    15721 aacaggaggg ccggcaacat aaatccgacc agggatacaa acatggccac ctgctgtttt
    15781 tccgtaatgg ttgagaccag caaacccagc gcaagggtat tgctgatgta taaagtattc
    15841 tccgccatca gcaggaacaa acttccgcgg atcggaaccc ctaatacata cacggcaagc
    15901 aggaggatga cggccacatc gatcatggca agcaccaggt aaggcaccgc tttgctgacc
    15961 acgatttgca tcgggtgcat cggggaagcc agcaggactt ccagggtgcc cagttctttt
    16021 tccctcacga tagctaccga ggtcatcatg gtgcaaagga tggtcaggat cagggtcatc
    16081 accccgggca caaaactgaa agcgctcacg agctgcggat tataaagcat ccgcgtttcg
    16141 atattgattt tatagggcag cttcatcagc ccgagcagtt catcctggta cgcgccgaat
    16201 atggccgacg cataactggt cgctatcgcg gccgtattgg gatccgaagc atccgccaga
    16261 acctggatct ggccggcatt gaggtgttcc aggtcattgg caaaatgagg agggaaaaca
    16321 atagccagtc gcgccgcccc cttttcaaaa agggcaggca ccaccgacgg gtccgcataa
    16381 tggcccatca cgtcgaaata ttcgctttgg tcaagccggt ctgtaagttt tttggtttct
    16441 tcatccctgg cctggtcaat gatcgcgacc cgcgactcct tgacttcgtt gctgagcgca
    16501 aagccgaaaa gcaacatcag catgaccggc tgagccaata agaaaaacag gctgcgggga
    16561 tcccgcaaca cgtgcttgaa ttccttgatg gtcattgccg caaacctgct attcataaga
    16621 ttccaaatga ttcatgcttt attctccctt cacccttcgc ctttaacctt tttcctttcc
    16681 tccctttctc cttttccctt tttctccttt aacccttcgc cttttacctt ttacctctcc
    16741 caggcacatc cggactcctc accgcccccc tcgccaattt cctgaacact tcatccatat
    16801 tttgactttc atattttttt ttcaggcttt ggggcgtatc catggcggct atcctgccgt
    16861 cgaccatgat gcatacccgg tggcaatatt ccgcttcatc catataatga gtggtgacga
    16921 acacggtaag tcctccttca gccgattcat atatcatatc ccagaattgc cttctggtga
    16981 tcgggtcaac cccgcccgtg ggctcatcca gaaacacgat cataggctgg tgctgaatcg
    17041 ccacggaaaa agccagtttt tgtttccagc ccaggggata atcccggagc agtttgttgg
    17101 catagggccc caggcccagg cggtcaagca ggcgttcgct ttttatcttc agttctttat
    17161 aagggatatc gtacaaacca ccataaaaaa gaatattctc cctcgccgtg agatcgttgt
    17221 aaagggaaaa tttctggctc atatagccga tgtttttttt gatctcctcg ctttgaaggt
    17281 tgatatcaaa accggcgacg cggccttccc cagaactggg tttcagcagg ccgcaaagca
    17341 ttcgcatcgc cgtggttttc ccggcgccgt tcgcgcccag gaagccaaag atctcccctt
    17401 tactcacatc aaaagagata tggtccaccg cggtgaaatc gccgaattta cgggtcaggt
    17461 ctttcacttc aatcgctttc atgtcctgct ggtcattttg tccataaaaa catcttcgat
    17521 gccgggttct atctgatcca gggccaccgg tccgggaatt ctcccgccga ggaagtccaa
    17581 gaggtcctgt gggctccccg atttgagttg tacatgatgg ctttccccga aagtgtaaca
    17641 ggagtgggtc tccggatagt cgcgtaccgc ccggagcaga agatacatat ccccggcgga
    17701 ggccgcgtat acaggccgtg acaggcggcg ggggatttcg gcaggagtcc cggtttccag
    17761 gatggtccct ttttgtataa gggctatacg gtcacaaaga gtggcttcat ccatataggg
    17821 tgtcgatacc aggatggtga ttcccctggc tttgagtcga atcagcatat cccagaattc
    17881 gcgtcgggat accggatcta ctcccgtggt cggttcgtcc agcagtaaga tttccggttc
    17941 gtggacgagg gcgcagcaca atgccagctt ttgtttcatg ccaccggaaa gttgaccggc
    18001 ccgccggttt ttaaagggag ccagttgatc ataaagatct ttgatcaaag ggtagttggt
    18061 ttccaggtcg gctttgaaga cggtggcaaa aaaccggagg ttctcctgga cggagaggtc
    18121 ctggtataga gaaaatcttc ccggcatata tcctatgatc tgccggaccc tggcataatc
    18181 ccggacagga tcgaaacctg ccaggctggc gctgccggaa tcgggaagga tgagactcgc
    18241 cagtatgcgg attagcgtgg ttttgcccgc tccatccggc ccgatcagtc cgaagatttc
    18301 tcctttttcc acagcaaaac tcacctcctg cagggcagtc acttttccct tgtcgaaggt
    18361 tcgggtcagc cggtcggtct ggatggtctt catttccatt ggacctcggc aaacatgccc
    18421 agtttgatcc ggccgtcgtt gaccactttt attttaaccg ggtaaaccag gttggccctt
    18481 tcgtccacgg tcataatgga tttgggagta aactccgcct tgtccgagat ccaggagatg
    18541 tttcccggat aggacaacac cgcctcccca ccctggtcaa tccataccgt caggtcctgg
    18601 cccagccgga tctcgcccaa ttgggttccg gtgacatagg ctttaagata gagggtatcc
    18661 aggtttgcga ttttataaag ggccttcccc gcattgacca tttcatgggc ataggcatat
    18721 tgggtcagga cggtaccgcg catcggattg gtgatcctgc atttttcttc caggtcttcc
    18781 aactgttcaa gcttcgccga aagaggcgat ttttcactca gtatcgccct gttttgaatg
    18841 gccgtattct gtcgcgcggc ctcgatctga cgcttcgtga tgtccacctg ggacttagcc
    18901 accgcgattt gtttttccac cacggacttt tgcgtccgca catcatccag ttgttttacc
    18961 ggggcggctt cggcatccac cagtcgctgt aaacgggttt catcgcggac cagagcggca
    19021 agctggtctt cgagaatccg gatctggttt tgattcaaaa cggcttgttg ctccagcacc
    19081 ttgatatttg ggcccgcatc cgcttttttg tcctccaggc tttccagaga ggccctggcc
    19141 tgctgaacag ccagttcctg ctggctgcag gaaatcatgc ccaccggagc gccggcatcc
    19201 aggaccatgc cttcctccaa atccaattgt aaaattttgc cggtggattc ggcggatacg
    19261 atgacctcgc gaccctcaaa aattccactg gcctgatagt ccctcttttt gttgttgcat
    19321 gccccggtaa acaccaggag caggaggatt aaacccggaa gcaaccggat ccgtgatggt
    19381 gaaaatttac cttttgccat ggatgagatt taatagttct ttggattgta acagctggat
    19441 ttgatgcata tcctttttga tccgggcctg ggtcaggttg ttggcatcgg tggcatattc
    19501 atttacggtg gcaaccccgg tttccagttg ggcttcgctg cttttcaaaa cccgttcatg
    19561 cagggcgatc accgcctcat cttccaggag gagccggccg aggtgttcga tttccgtctc
    19621 ctgggcccgg atccggatct tttcctggag attaaaggat tcttcctgca cctttatctt
    19681 gtccagcccg gcttgcagca ggtctttttc ccgctggccc tgccggctgt ataggttatt
    19741 aaggttccac tgccatttca ggccaaggat tgtgtaaggc ttgaattcat ccgacaggag
    19801 attcaaaccc ggacggccat aacccaattg accaaagagc tggactttgg gttgaaatcg
    19861 ggtttgtgac aaactaaatg aagcggcctg aaatttccgt tgggcctcaa acaaagccag
    19921 ttcgggacgg gtaccggcca aatcctccgg actgaggctg ggtacaaact gcatgcttga
    19981 atcgagggac attcccgtga gcagttccag ggaggacaaa gcattggact tttgtttttt
    20041 tatttccagc atggcttggt ttgtctcccg gatctttatt tgcaaggccg cctcctggga
    20101 tcccgccgcc atgccttccc cgatctgcct gtttaggcgc gccgcttttt tttccaggtc
    20161 ttcagccagg agggaccatt gtcgaatgga aagatcggcc aggaggacgc tgaaaaaagt
    20221 cttacaaacc agttctttca agggatggat catcacctgt gtgccggcca ggtccatatc
    20281 gtaggcccgg acactctgtt catcctgggc tttgcgctgg cctccgtcat agatgagctg
    20341 ggacaattca agcaatgccc ggtactgatc ccggggctgt cccgggatat ccagcccggg
    20401 aaggacgacg gggatttccg tcacatccga ttgccaggaa gcctggccca aaaagctggc
    20461 ctggggaatg cgggagaggg cattgatttt tttctggttt tcttcggtcg atttcgcgat
    20521 ggcggccatt ctttggtaag gatggttttt ttcaatggcc gcataaagat cgacaaggct
    20581 gaccctgttt tgagaaaacc ctgttaacac aacagcctgg atcaaaaaca aggcaaggac
    20641 gaaccgctta agcatatggg catgtttcat cggaacgggg atttattttt tgatggaatt
    20701 ccagatcatg gtcactactt ctttcttttt tttctgcatg aggctgttga atgatttttc
    20761 ggtgaaaccc agtttaccgg tcaggatggg tttggccagg aatggaaaga tggtcatcgc
    20821 ctggatgtta aggatcaatt cgcgggctga gatataacgg ataatacctc gcaggctttc
    20881 ctccttgatc tgtttttcaa gctttgtaag gacccggttg accgggatgt tttccatgcc
    20941 cttaaagatc atctcaggat ttcggtgcag ttcgctcagg ataaagactg gcaatcccgg
    21001 catgccgagc agtttgtcga tatcgaggga aacgatttgt tccagtttct tttcaagcgg
    21061 ggcttcttcc aacaagactc cggcaatgga agaaaacagg ttcctgaatt cggagaggaa
    21121 gatggagtca aacagttttt ccttgctgcg gtaataataa ttgaccatgg acttggtggt
    21181 gcccgcttcc tttgcgatct cctggaggga agcgccagca aatcctttcc ttgaaaaatg
    21241 tctccgggcc gaagccagga tcatttcttc aatatttttt ttcacgacgg acatgtcgaa
    21301 cacgtgtttc gaacgtgtgt tcgaatttca aagatataag gtttttgttt taaaatccaa
    21361 aaaaaggtta tcggtacgct gactcccggg aaattacctt gttgagtatg aatgcgagga
    21421 aaggcgggtg gatgcccttg ggatcaattc attattcact gcattagatt tactcctttc
    21481 aatcacaata caacttaaag ggcttaagag accgcgaacc ggtcaaactt actatatata
    21541 cgccggcgcc gggaggcttc cagtctatcg tgatctcgcc aggataggtg gagggcaatc
    21601 taacacggtt taataatctt ccatccagcg aataaacatc catcttatca aaaggctcca
    21661 cctgactcaa aataaaccta cccttgttat aaattacttt aggatttatt ggattcccat
    21721 gctctgaggt agaggcgccc cggggccgga atagaaaaaa catatatcgg atagtatcac
    21781 tgctgttcaa attgtaatgc aaggataaac ttttcgccat ttcatgagaa ataattaagg
    21841 aatcctgatc tctcaataag aggatatcgg aataatcacc tgttagaggc ccaattattg
    21901 atggcgcaaa acaacttctg gccacgcaaa tgccattaaa atcagtctta ttccatttca
    21961 aggtcaaggg aaacatatac atattcactt ttacaatcaa ctgcaagggt ccatattcat
    22021 attcgccgca ttttggtgaa tgtataatgg agcggttaaa agaatatact cgtaatgggt
    22081 gtaatataat atcaaaactg tcccgaaata atatactctg attcgggcga tacaaatcgt
    22141 actccccaaa tattgtatct ccaaattctt cataggcatt cctgtcgaac ccaatagtaa
    22201 attttaccgc tttcccctgt gcattagtca tcgtgaaatc ggattcaaat tccgccattt
    22261 tctgggatga tgccagtaaa ggagcaagaa atcctatagt gcacagtata atcgaaattt
    22321 tcataaccct aatgatttga tcatgccagt ttgatgataa ctgctagagc ctttatttca
    22381 attttggatc tgcaatacaa atataattcc tgaatgcaga gataatgtta aagtgcaaag
    22441 cactggagga atagcatgaa aatccgcact tgacccccta ccatgaaaca ccaattccca
    22501 gcctcaacaa ccttcccgcc ccgttgatcc ccgaaccatg cacccggtaa tcctgattaa
    22561 acaggttttc caccccggcc ctgcattcca gcgacttcag ttttaccgtc aggcccaaat
    22621 tcagaatatt ccaggcaggg gtgcctgtcc ttgaaatgcg gttgtcggaa atatcaccgg
    22681 cgctgagccg gtcttgcctt cccgcattga gtgattctgc ataaaaactc cagcgcagcc
    22741 ccggctgata ctggatcttt ccgatacaaa acaagggagg gattctccgg atcggttcat
    22801 ccgcactttg attctgacca aaggaatatt ggatcattcc tctgacagtc cagcgactgc
    22861 tgaatttcaa attcaaggaa gattccagtc catagatata ggaccggtcc gcattttttt
    22921 tgataaaaac gggatatccc tggatggaat cgcccgaaat agcggttcgg gtgatcaggt
    22981 catgcagttt cagataaaaa agagacgttt ccaattgaag cgacttgctg acccagcggt
    23041 ggcctgctcc aagatgtaat cctttttccg ggctgagttc ataattgggg acttcgtacc
    23101 tgaaatcgac aatccccagg gtgcctgcat catcgatatt gggcgcccgg aaagttttgt
    23161 cggcatgaat aaaaatccgg tgggcgccca tcgtcctgcc aagggctaga tgccaaacca
    23221 gggctgaagg ttttaagacc acctcgccca ggctggcctc atttgttttc agttgataaa
    23281 tattatacct ccatcccagt gcgaggtccc aggattccca gtcaaaattc cagtcattaa
    23341 aaaaacctgc attcatgtaa gtcgctccat cgggataaag gccccgcaag gccggactgc
    23401 ccggaaccgt cgtttccaat ccggcagtgc tattgacctt gtcccggtat aagtctaccc
    23461 cgaggatacc gctcgtattg tttgaaggac tcaactcaag ctgcgaggaa aatccccaaa
    23521 ccccggcaat atcttcttcc ttcctgaccg ggtccgtcgt ttttttctgc aggctcctct
    23581 gttcggacaa tttttgccag gacagggtag tttctatctg tttaaaaaaa cgcgagcctc
    23641 cgaattcata tttattgcga aggaaggcca ggtgatggct gagctgatcc gactggttgc
    23701 gttgaaatcc ttccagcacc actttgtgat aaaccgggac ctgcgtctgc cgggtggccg
    23761 accaggaaag gtcccaggcg ccggccgggt tttcccaccg ggctttgaga tccgcactgc
    23821 cttcatcata accactgggg atttgcttgc cggttttttt gcctccctgc agatccccgt
    23881 aaccggctaa agctccgcgc atcgacaaac caaatttgtt ttttgaaaac cggatccccg
    23941 cattggccag tttttccacg ccggaggaag cccattgggt gtagacattt ccggaaaagg
    24001 tctgcccggc accggtcacc ggattcgaac tgatcaggtg aatgaccccg ccgatggcat
    24061 cggagccgta ttggaccgcc cccgatcccc ggatcacttc tatctgtccg ggctcaaaga
    24121 gcgattgcag gctcaggtat tgattggggc cataacggta gatggaatta ttgagccgta
    24181 ttccgtcctt cacgatcaaa acctggttgc cggtaaggcc gcgtataaaa ggggatcccc
    24241 cgccctggtt ggttttttgc atccagatcc cttcgtgttg caaagcttcg accaggttgc
    24301 gggaggcgac ctcgttcagt ttttcacgtt caattctttg tacgggcata ttcaccgatg
    24361 ccctggcctg ggccatgcgg gatgcggtga caacgacttc cttcacttcc agaatggtgt
    24421 cggacatgga ttgttgtgcc tgggcgccaa gggcggtttg aataaatatg aggcaaccta
    24481 cgattgatct ccgcatctcc cgggattatt taagatattc ctggatggag gtgcgtataa
    24541 aggtctcatc gtccagtttg gggccggtac ccagggcttt ccagtcgaat tcttcattca
    24601 aaggagtcag ggccttgcgg ggattaaaaa ccagggtatc caccatggtt gcctggtaag
    24661 gggaaaaatc aggctgggtg gtgaaacact ccgccaggtc ggtggcgcct gcatcgtatt
    24721 gattcagata aggaatgccc aggatgttcc agaaggtctt gaagatgctt ccgaaactgt
    24781 aatgagtatg gcttacgtaa ttcttttttg cataaggaga gaccaccatc aggatgctgc
    24841 ggtgggcgtc gatatggtcg cggccatcct ggctgtcgtc ctccgtgatc acaatggcca
    24901 tttctttcca gtatggggta tgcgataaaa attcaaccag gcggcccagg gccaggtcgt
    24961 tgtccgacat ataactttcg cggaaaggga agcccgcctc cgggcggtct ccggcgccat
    25021 gatcgttggg aaggatgatg gtgatcacct ggggcaggct cttcccgtcg ccccattttt
    25081 cattaaattc ctcgatgaaa acatccagcc ggaactggtc cgggatagcc atattgtagg
    25141 tggcataccg gcgggaagtg cggtcgtaca gcggcccggg caagggataa ttgaccaggt
    25201 atttcacccc gccgtatttc atggacggat cggcgaaaga gccggcgtca aattccaccc
    25261 caaaaccaaa attgaaaaac tccttgccgt ttcggtgcag gtgatcccag atggacccat
    25321 gcgggttgta ttcttccggg tagatggcgc cggaagcccc ggtcataccg aaacggccgg
    25381 gggcgttgga acggtgattt aggtctcttt tacctccgta agccgcagac gtatgggtct
    25441 cgagccattc attgggatag gtgcccgcca gccagcggtg gccgtccgcg gaatgatcgg
    25501 aatccacata aaaattatcg ctgatggcga agcgctgggc cagggccaaa tggttgggca
    25561 tgaccgtggt gttgatcacg gaatcggttc ttttcttatt gtaaaaacta acccggtgcc
    25621 cgtaacgagc catggaaggc tcccccttgc cgtttttgat ttgtccgaat acttcgtcat
    25681 aggtcctgtt ttccttcgaa acaaaaacaa tatgtttgat cgggctgggg atcccggagg
    25741 ccggatgagg aggcaccggg cccattgatt ttttatccag gggtttccag gaaaaattat
    25801 tggcgatcac ttccccggta aactttgaca gctcagcctc cgaaggaata tccagtatgg
    25861 taaccgatcc tttcatgagt gaaccgatat aatctccctc cgggcctgag gtataggagg
    25921 gcccgccatt ggggccgctt ccaaatcctt ttgcattggc caccaggagc tttttcccgt
    25981 cggggcttac ccggagcttg gatgggaacc agcctacagg caaatggccc tggaggctca
    26041 gggtattgag gtcaatgacc ccgaccgcgt tgatccccga ttccgcaacg tacaattttt
    26101 tcctttccgg atcgaggctg agcccaaacg gaatgatccc tttcatgggt ccaagacggt
    26161 cgtccagtgt gaggtcgatg cttttcacca ggctgtcgcg ggcggcatcg atcaccgata
    26221 tacaatcgtg gcttccgttg ctgacatata catattgacc gtcggtgact atggaattgg
    26281 gactgctgcc ccccacggcg gggatcccat cgagtttttc acctacccgg aaaccggtct
    26341 ttatcctagc ctttggggcg gactgtggcg actcaaggtc aaagacccaa atcgaaaagg
    26401 cttcgtccgc attcatctcc cccaggcctt tgacctggat cgaatcgcgg tcaatgcctt
    26461 tgaccatttc ttcagagcca taagcaaaag caggaaaatc caggggccgc agcccgacgc
    26521 tgttggaatc catattgttg accaggctgt attcaaacat gcccacattg gccacataca
    26581 tctttttttc atccggagag aggcagatcc caaaaggata tcttccagtg cgccagttcc
    26641 gcaccacgtt tccggtttcc aggtttactt cgatcacccg gaacccggtc tgatccacgg
    26701 catacagggt ttttccgtcg cgggtcatga tcatgtcccc gatatacccg tcattgtatc
    26761 tgaaaccatc ctgatccgaa gtacaggaaa tggttgattt tttctctccg gacaaggcat
    26821 caaaggcgaa tattttagtg gtctgccctc ccgccacata gatggtttta tcgtcagggg
    26881 aaatggccag gcccataaac actgcggaaa gcaggtcgtc atcggtattg gcggatttgg
    26941 ggatctgcaa ggtggtcatg gaatcggtaa acgggttccg gagcaccgtg atggaaaagg
    27001 ggttggtccc cgaatttgcg gtcactgcga gacgcccgtc ggctgaaagc accaggccat
    27061 aaggatgggg agcgattcgg accgtctttc ccatgggcct tacgtatctt ccattgggca
    27121 ggatggcttc gcgctcgttg ataaaacaat attcgtttcc ggcaggtgcg gaggataccc
    27181 agatctggtc agcggatttg ttccggcaag cgaagcacaa gaaaaggaga aggagtccga
    27241 tataaataaa tggtttcatg ttttggaatt caattggggc ccaagttaga ttttcgtaaa
    27301 atatccgggt tgtattgaaa tataactaaa tgaataagtt tcttcggtcg gaccatcctc
    27361 cgggatgcct tagagcaaat gaacaacata acccggcgca ggaaattctt ggttttagga
    27421 gatatccact tcccggtaag cctggatcag tttcatctcc ccttccttgc cggggaaggc
    27481 gttgccgtgt tccatgccca tgacccccgt ataaccgcgt ccgtggatgt atttgaatat
    27541 gtttttataa ttgatctccc cggtattcgg ttctttccgg cccggattgt ccccgatctg
    27601 gaaataggcg atctcatccc agcatttttc gatcgtgggg atcagttggc cttcattgcg
    27661 ctgcatgtga taaatatcgt aaaggatctt gcaggccggg ctgttgaccg ctttgcagat
    27721 gaggtaggtt tgcgccgcat gccggaggaa cagatccggg ttatccgaaa gcggttccat
    27781 caccatagtc aatccgtgcg gttcgaggac ttcggctccg cgccgcatag cctctatgac
    27841 attggcggtc tggatgtcga ggggcaggtt tcgttcaaaa tagccgggca ctacagtcat
    27901 ccatttggca ttgaccctct tggccagttc caccgatgcc ttgcaggtgg caacgaattt
    27961 gtccaggaat tcttttttcc cggtggccag ggatattttc cagttgtcgc ccccctcaat
    28021 cacaaaaacg cccatcctca tgcccagggc ggtcagggtc gagccgattt tatcctggac
    28081 ctctttgggg cgggccagca tgccattgtc ttccaaggac cggaatccct ggtcggccat
    28141 aaatttcagt tggtccacca ggtcctcccc ggccgagttt ttgaacatgc cgaaatgcgg
    28201 ggcataatcc attttaaacg cgggagcgga agtctcttta aggtttcctg agaggttggg
    28261 catacctgct atccctgcgg ggacggcgag ggcggcggac tggataaatt ttcttctttt
    28321 catgattcaa atgctggttt gtaaacctaa taaaatcctg ggtaataaca gggagtttaa
    28381 tttaatagat atctgtcgaa atgctcccgg agcgaggcca aacaaatgat gccccttcag
    28441 agcgaatagc tctattgagc ccctagcatt gggcgagacc gcccaatgct agggggataa
    28501 gggcctttca ggccgatatg gcagaatgga aggcgtcctc atcagcttca taaattacag
    28561 gcaagaaggc ttttcaggcc gatatgacag aatggaggcc tttaaaggcc agggggaatg
    28621 tcggaatagt tttaaacgcc tgaaaacaat ctcgtttcta caaaatattt ttcactatac
    28681 ccaacttgta tttaatctgg acttcccagt cacccccaaa ccgtaaactt caattgaaga
    28741 tttgtgtttc atccagctac aattttggaa tgcatcctat caaccctact aatcctacca
    28801 atcctacaaa tcctatccaa tcctaccaat cctattaatc ctaccaatcc taccaatcct
    28861 attaatccta ccaatcctac
//
LOCUS       DAOQXT010000058        28665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1919_length_28665_cov_7.857777, whole genome shotgun sequence.
ACCESSION   DAOQXT010000058 DAOQXT010000000
VERSION     DAOQXT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 28665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28665
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1919_length_28665_cov_7.857777"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /locus_tag="PKM27_11095"
     CDS             <1..2099
                     /locus_tag="PKM27_11095"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07851.1"
                     /translation="TGGNTWSTGATTDSIYVKTAGSYTVIYDDGTGCGTTLSDTVNIT
                     VFTPPVVSISGTLGICPNETTVLHAGSGFASYLWSTNETADSIVVGNPGLYIVVVTDA
                     NGCVGSDTASTQFSPVPAPSITGTFLFCPGDSTLLDAGTGFSSYLWSTGASTQTITVS
                     TEGGFSVTVTNSDGCQGSTTEFTSLYPSPFAVINGYDGFCPADSVLLSGSGGQSYLWS
                     NGSTTPDIYAKTAGLYILTVTDANGCKDSISKDIVEFTPPNPFISGTLSFCAGGSVTA
                     LDAGPNFNSYLWSTGESSSSILVDRVGTYDVTVTDNNGCHGSTSATVTQEGGIPDVPG
                     PITGSLFGMCNNLSPSTYSVSPVPNSTCYLWKMPPGATIVSGFMPDSNLFHNVIQVVF
                     DNSFTGGYIEVASHNDCGASPSFNGRSIYISAAPGSVPSNITGQASGVCKQVSTMYSI
                     SPVTGAVSYEWIVPLGAEITGYNGDSTAIEVFFATSYRTGDICVRYTTDCGTSPYKCL
                     NVSPQPKIQSNIAGPTTVCAQMQNAMFNIPPVIGATAYLWSVPTGATIVSGQGTTQIS
                     VNFGNHTGIVTVKAINDCGNSLIQYLSVNVTNCTRFPSREINYTKRRPNDFKFELYPN
                     PSDGLFLLELTSPEPGTEYALTVSDALGKKVLEYKLPGNQFVRDWLDLRHLGKGMYFM
                     TIANKSQQQTAKLVIH"
     gene            complement(2105..2974)
                     /locus_tag="PKM27_11100"
     CDS             complement(2105..2974)
                     /locus_tag="PKM27_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNR07852.1"
                     /translation="MIKSWISALIHSNILIALCAVALALETQVQLGLRPHVHPYLFLI
                     FFATLFEYNLHRLITVVYVPEALEDEKYEWLRQHKPGFYLMVFLSVTGFVITLFQAKK
                     EVLIALAPLAALALFYSTPVRHLGKKWFRLREIPYLKIFLIAGVWSGVTILLPVIHSG
                     RSYQVPFLAALLVERIFFILAITLPFDIRDLEADRKVGLKTIPMLFKKTNPLVLSYLC
                     LAMFAAIALVQYPGMGMSYITVALLISALTTAFFLASEKLQVLPFYHLGILDGTLLLQ
                     SVLVLVFYFLFKS"
     gene            complement(2977..4395)
                     /locus_tag="PKM27_11105"
     CDS             complement(2977..4395)
                     /locus_tag="PKM27_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNR07853.1"
                     /translation="MNDRMKNAESMSLSAGVHSANLSPVQKGALGLVGSGILIQLIYW
                     ISFKTQNASVWLWTSILLVSAGSVWYSYETYHAHPPGIKNNGVWFRSLTARGSLAWLL
                     AVILTGFYILLYWYPQYLGQGQGEGGANSGLIRLFDPLSQYLKGKPASQWFVYGTFYT
                     LIMFLMGIKFIYKYRHDRYQVLRTLSVLFFQTAFAFILPEILQGLNRPYFDFKNIWPL
                     NYYFFFDWHIDGLIQSGMLGYFMLFWGIVSFLVLTPVLTWFYGKRWYCSWVCGCGGLA
                     ETAGDPFRHLSSSSLKAWKVERWMVHGVLVFVVVMTAMVLYTRFTGAANILFLNSYQL
                     SAWYGFFIGAAFSGVIGVGFYPLMGSRVWCRFGCPMAAYLGILQKTKSRFRITVNGGQ
                     CISCGNCSTYCEMGIDVKSYAQRGQDVVRASCVGCGICSAVCPRGVLRLENGILDAGY
                     RTRTKEELDYEERSGMKPGVNK"
     gene            complement(4388..5749)
                     /locus_tag="PKM27_11110"
     CDS             complement(4388..5749)
                     /locus_tag="PKM27_11110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNR07854.1"
                     /translation="MRIIIIGNGIAGSTAARFIRKYSDHEILMISEESEYPFSRTALM
                     YVYMGHLRLEHTKLYEDGFWAKNRIDRLHGRVTEINPVSKSVQLHNGQSLSYDILILA
                     CGSLPKKFDWPGQDLQGVQGLYHLRDLQQLEDQTGRIRKAVIVGGGLIGVELAEMLRS
                     RNIAVSFLAIESSFWNTVLPAEESAMVNEEIRRHGVDLRLNTGLEEIYNDGRGWVAGV
                     RTGHDDHLEAQFVGLTVGVRPNIGFLYNSDIETNMGVMVDDYLRSSQKDIYAIGDCAE
                     LWTPRPGRKAIEAIWYTGRIMGETVAHTLCKGPVKYDPGIWYNSAKFFDIEYQVYGEI
                     EPQPVEHVQSLYWQGANGKKSIRINYNSATSPNFPLAVRGFCLMGIRYRQEVCEQWIR
                     SRATIETVLPQLGLANFDPEFFPQYESRLMDLYYIQSGKRLILKSGRDRDAVTRFLNK
                     INE"
     gene            complement(5801..8716)
                     /locus_tag="PKM27_11115"
     CDS             complement(5801..8716)
                     /locus_tag="PKM27_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07855.1"
                     /translation="MRLVICFFFLVWKLQLIALPGTADTTGISIIYTGRSLGALGVMR
                     SQEEHELVTEQANTEHKPFKLVSHSCWRAPGLAVFLPSDEPRGNELPRILAQKDSAKA
                     LKKYPAFKTHNSLLVQDPLRRNPDMLDMMKKNPRQKTDIPDLAETQVTYYSMTIRNGD
                     PAIIIEEEGAVWPEHPDLWTIGEVNRMDIGDTGRLYELPFNLGEMAVRSTVIKKQWVN
                     ADQNGEYQILADLGHRTGNFGLDDLERARIDFSLLTKLGYSILVPYHFELSLGADSLK
                     RLSLDFPGIRLLATNIRSRDSSLFHSRLILPFGNIRVGLLGLVDPTLQGDLSKNSLKD
                     FQFLPYLETIRYEVNQMRKSGVEAIVVLSNLDAGDNAALAEKVAGIDAIVADLHSRLS
                     PETVIQSVELPGHNLNRPGSPALVTRSFADGLGVGRLRMKFDAEGRLLGLQHRLDPVT
                     DQTVPDTSVIRQILSLSQRVSKPKGDLLFPAFNDLVTLYPDLKKYDATTQQGRVSQRM
                     WEEFVARLLRNAGPTELAVIRKFPYFPPLIGKLHEREVSAWLWNEDEILFCDVKGSAL
                     LKILADDVHEDLIFSGLTRPKPGPQQTYSRGLPSVSMEWLQLNCRILGRPVDPDGFYR
                     VATTDVIFEGVRAADFLESRRVRRQFIMDKEGRLVESPEGDKLSLRSFVLNELKRLNK
                     NNKGDKFLQAVTERLKPDPRFEPLLSFDFDRPTLWTSYNRKYNSDGYGAVPESRITAT
                     NSWVIGASGRFKATMDGLKNALDLGLTLAYAKQNSEISKNIQQISESADDIKLDLTYR
                     RKSNRIFQPFVRGQYDTEFTPTINPATLEENYKQQALRGVIGLARRFHRNWRNVELAS
                     VLEQDFGQNKAQFGFTARADGRFFLGGGAVIYSLRNDATWFFKAKSDTNRDLALRYNM
                     VHELLIPLIDELSLSVASDFFFFKGKVEETRETGMSMLLRVGITYDRLWKPRYQPLF"
     gene            complement(8803..9345)
                     /locus_tag="PKM27_11120"
     CDS             complement(8803..9345)
                     /locus_tag="PKM27_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNR07856.1"
                     /translation="MRKIILQLAVSLDGFIEGPNGEYDWCLTDQDYGMSSFLKKVDTL
                     FMGRKSYDLAKSMPVAAEDPFMVKMSKIKQYVFSNSLKEDEVQEPFHLVSGDTVSWTK
                     QYKQEKGKDIWLWGGASLTTSFMDHWLVDELVLAVHPILLGDGKPLFHGLKHRVRLDL
                     KSSRKYPSGLVMLRYAIVND"
     gene            9404..10549
                     /locus_tag="PKM27_11125"
     CDS             9404..10549
                     /locus_tag="PKM27_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HNR07857.1"
                     /translation="MRKSLSKLPLVGTNIFTKMSGLAAEVGAINIAQGFPDFACNPEL
                     IALVDQAMKGGFNQYAPMPGLLKLREGIAEKTFELYRREYDPGTEITVMPGATAGIYS
                     AIQALVQPGEEVIVLEPCYDSYIPSIILAGAVPVRYKMKFPGYAIDWEEVRRLINSRT
                     RMMVINTPHNPTGNILTREDMLALQSLVRDTDLLVLSDEVYEHIVFDGEKHWSVSAFP
                     ELAERSIMVASFGKTYHATGWRVGYVLAPAEMTAEIRKVYQFIAFSASTPVQHALAAF
                     LNKKEAYLQLNLFFEKKRDRFREMISGSRFSLLPSRGSYFQCATYERITDEGDAELAI
                     RLTHEYKVATIPVSAFYQDHTDHRVLRFCFGKKDETLEKAAEILCKI"
     gene            10537..11331
                     /locus_tag="PKM27_11130"
     CDS             10537..11331
                     /locus_tag="PKM27_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR07858.1"
                     /translation="MQDLSLTLLQTSLQWENPEANLAHFDRLISRIQGPTDLILLPEM
                     FNTGFSMRADSLAETMEGPTVNWMKTKAAQNQTVVFGSLIIREEKAFYNRLVAALPDG
                     SLQYYNKRHLFRMAGEQLTYTGGNELLTLEIKGWKIRPFVCYDLRFPVWSRNSFDPLA
                     GWQYDLAVYVANWPSARAHAWKSLPVARAIENQAWIATLNRIGSDGKGHTYSGDSQVI
                     NSYGETVVHLDEAEYVQTVVLDRNSLEKHREVFPVGRDADRFEIFP"
     gene            11338..11856
                     /locus_tag="PKM27_11135"
     CDS             11338..11856
                     /locus_tag="PKM27_11135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNR07859.1"
                     /translation="MNLTLRREKASDFHGIYLVHQAAFRQEAEGRLVEKLRKNKAYIP
                     ELSLVAIQDNLVVGHIIFSKIKISGGQKDYESLTLAPLGVLTEFQNKGIGSLLVREGL
                     KKARELGYRSVIVLGHKNFYPRFGFVPASRWGIESPYEVPDEAFMAIELVPGGLDGIS
                     GKVKYDEAFEEL"
     gene            11923..12399
                     /locus_tag="PKM27_11140"
     CDS             11923..12399
                     /locus_tag="PKM27_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HNR07860.1"
                     /translation="MKARNAIGLDSKKSKQLAEKLNDLLAHYSIFYQNTRGYHWNIKG
                     EKFFELHLKFEELYNDLLLKIDEVAERILTLGYAPLHNYSEYKKASAIKESSQVSDGL
                     KAVEDILNSFRVIIGLQRELLGLSAEAGDEGTNALMSDYIRAQEKLVWMYSAFLNK"
     gene            12437..13063
                     /locus_tag="PKM27_11145"
     CDS             12437..13063
                     /locus_tag="PKM27_11145"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HNR07861.1"
                     /translation="MKAYKKYFDQALRLNYPGQAVDYIRETEFLYQGIRADLGFVSSS
                     GNPMDRRLYISGYFLALIQVLDRDGKSMEEIRSLVTGIASEYVRPKNKWQAFLKRLPA
                     RLIKSGLASILLKKMDRMVSRNAHPEGFRVRVLTGKEETLGFGYGVDILECGICKLFA
                     RHGFSKYAALLCEVDYITSSLAGLQLVRTGTIANGAEQCDFRFKRTGG"
     gene            13298..14263
                     /gene="ric"
                     /locus_tag="PKM27_11150"
     CDS             13298..14263
                     /gene="ric"
                     /locus_tag="PKM27_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNR07862.1"
                     /translation="METVTDNMLDVTLLEPRMKHPTIFAGFDALAEGQSLVIHNDHDP
                     KPLYYQLLGERGNVFNWEYLTEGPRWWKVRITKRRADEQDETLGQIAAKDLRKAEVFK
                     KYGLDFCCGGKKTVKQACAEKGLDVTRIEQELQQADKLPTAQALPYQEWSLAFLSDYI
                     INTHHLYVKKSLPELEAYAIKVAAVHGGNHPELAEIKALVQDLKAEMTTHMAKEEKIV
                     FPYIKNLEGVASRGVAVFDSITQPVHQMEIEHESAGLLLEKIRQLSHDYQAPQDACGS
                     YRMLYSMLEAFENDLHLHVHLENNILFPKSIELEKKSKSIQVQSA"
     gene            14266..14790
                     /locus_tag="PKM27_11155"
     CDS             14266..14790
                     /locus_tag="PKM27_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNR07863.1"
                     /translation="MVYRRTEPASPIRRHPSIVPFSRDHHFGLLLSWKIRQGIKNHID
                     GERISDYVIYFFEKALAPHFRLEEEQLFPLFAEGHAGCQQALREHTQLEFLVNELRRD
                     KTGNVILQAFADLLEKHIRFEERILFNQLQTEIYDPHITEDRSQHLTKEEDPDLNWED
                     HFWTASPEKRRRNK"
     gene            14787..15593
                     /locus_tag="PKM27_11160"
     CDS             14787..15593
                     /locus_tag="PKM27_11160"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNR07864.1"
                     /translation="MKPINPDTKIAVLLKQDPRALEAIISLSPRFEKLRNPLLRKLMA
                     GRTSIQMASRIGGVNIRDFLSKLEALGFYIEGSGPVPAESKNRETPDFLKDLPPDRIT
                     ELDVRPEIETGKDPLHLILQRLKDLKPGQVLKLVNSFEPVPLIELLQKQGYESHVEEI
                     SPDLVITWFYATRPVDIQGLDPAKTKGWEILLKRFDQKMQTLDVRQLEMPLPMLTILE
                     ALETLPPDQALYVYHKRIPLFLLPELAERHFDYRIKEVSDQEVHLLLFRS"
     gene            15595..16887
                     /locus_tag="PKM27_11165"
     CDS             15595..16887
                     /locus_tag="PKM27_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07865.1"
                     /translation="MFGVPVSSVAQKTTSHKVVLPFYGYAAASLFAAALLILANSAAF
                     TGHYFHPRILAITHILALGWATMIILGASHQLVPILVEGSLYSEKLAMASFILAGLGI
                     PLLSYGFYYFEMGWPAKWGGRLVVLAILCYLINLFKSISRGKQDNVHALFVFTAGLWL
                     FITAFFGLVLVYNFTYTLLSEDTLHYLPLHAHTGILGWFLLLVMGVASRLIPMFLISK
                     YRNPRLLWLIYLLINTALLGFFFLYLSTVLINLIFIPALMVLAAVFLFIYYCYRAYQA
                     RLRKRVDPQVRISLFSVGLLALPWMLLLILIGLLLRNGGEQTSLALAYGFLVFFGWIT
                     AIILGMTFKTLPFIVWNKTYHHLAGIGRTPNPKDLFHDGIFRVMVLAYLAGLIFFTAG
                     ILAVQLFLLQAGAVLLLGTAALYNWNVFKLLFHKKPVT"
     gene            16884..17207
                     /locus_tag="PKM27_11170"
     CDS             16884..17207
                     /locus_tag="PKM27_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNR07866.1"
                     /translation="MNPVTNLPEKSKLALEALHQVLDPEIGLNVVDLGLIYRLDFDEE
                     KKELFCTMTLTTSFCPMGESITFGVLQALEACFPGYAVHVGLSFDPPWSQDLISGEGL
                     QFLNR"
     gene            17261..17692
                     /locus_tag="PKM27_11175"
     CDS             17261..17692
                     /locus_tag="PKM27_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNR07867.1"
                     /translation="MLSLTCKAAIKAVIFLASRLEAGQKTGILEISSHIGENEHTVGK
                     LLQKLVKEKIIHSAKGPRGGFYMTAGQQDQPVIRVVEAIDGKAVFDQCGLGLSRCSAT
                     HPCPIHDDYKVVRDAFKKMCEERKIRDLYGPVHRGTAYLLG"
     gene            17759..18412
                     /locus_tag="PKM27_11180"
     CDS             17759..18412
                     /locus_tag="PKM27_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07868.1"
                     /translation="MSRISSFSAPHKGLRNLLSKFSFLAGKTNFEDPAQVADLKKLGF
                     DLFRLLDEHARLENEFILATLAERVPHGADHDLRDHEYLEEEQDKVSHKLKRLNGKQD
                     ADAGHDFYLSFSKYHSRYLAHILQEETVTEKLLQENFSDEELLQLRGRIMKSIPFSDF
                     LIWVKYIIPAQNESENIGMLKGFIASAPPEAFQKVSEVLRKELPEAEYSSLIQKVTG"
     gene            18533..21241
                     /locus_tag="PKM27_11185"
     CDS             18533..21241
                     /locus_tag="PKM27_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNR07869.1"
                     /translation="MISTPDQRVRVFVSSTLEELAEERKVVRKAIEELRLIPVMFELG
                     ARAHPPRQLYQAYLEQSDVFIGIYWNQYGWTAPEMDISGLEDEYRMSGSKPRLIYVKS
                     STSRDPRLDRLIRDIEKQGSLAYKLFQDLRELQEYIENDLAALLSEKFRSETQKESPD
                     PKIFFNNIPQPYHELIGRQEEKKNLEDLLLGQHKRLITLTGMGGTGKSRLAIEIGFAA
                     LAHFEHGVCFVSLGSIRDHDQLESCIALALGLSNASSQALRETLLSYLSDKRLLLILD
                     NFEHVLEGAGLLSDILSRTRQVSILVTSRSSLNLRAEWIFPLSPLPVSSKVECGESGN
                     IELPSLKLFMERANSVNPRINWNTDNLEAAAVICFRLGGLPLAIELAAMRTKYNSPVQ
                     LLKGMEKSLDLLTGGARDLPGRQQSFRATLDWSYRLLSPEDQEIFRIMSIFQDGWYAD
                     TLRSVSGLPAETCAAATERLLDAGLIRLKNIDPEPRFDMLQPIDEYAGELFESDMEKD
                     PVRDRFLQFYVDLAKDISPSLLTVNYNRNWLPMLRTDYENFREAFQIALFRQDKKSAA
                     TLINAVGTYWMFEGHQNEGLLWLSQAGLNEPGDWRTLSKEEVHVYANSMLCSGIFLFF
                     PARFEEAIRDLKIGRDMFETIQDYRGMSRCLTYLGLISLSTGDLEGKSYFNEAIRRGN
                     ESGDYFSVFISTTFLSEYYLLTGDSAQAIQQIGEIDFLLNQRYTGPNYAVMQSYQPIF
                     YVEKGNVFLYAGDLETARQAFEESIRLFDQSGLKTSKGYAFVGLAIVHGLRGDYAHAK
                     KTSLRGLSTGRELGDVIIILVNLKVFLSSLIGEGEWKSSLPLLDEVFAFIRHYKYASW
                     TGDKITDAWIQGLLDQKQLKTNPGTDVPKITLDELIQKTLSLATEG"
     gene            21450..21986
                     /locus_tag="PKM27_11190"
     CDS             21450..21986
                     /locus_tag="PKM27_11190"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HNR07870.1"
                     /translation="MEVKTNSRTGQILDFMYILAWVAFIGLMIEAGAILISYGVSCVN
                     PEAARNLYKGMDLYPLRHYSFWHYTQSVSLIFALSAMKSFILFLVIKTLSKVNLKSPF
                     TIEVARTLEKISYVFLGTWIIAIINNAQNEGLLKRAGIFIEDRDAGEFLFMAGLVFII
                     SQVFKRGVELQSENELTV"
     gene            21990..22196
                     /locus_tag="PKM27_11195"
     CDS             21990..22196
                     /locus_tag="PKM27_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR07871.1"
                     /translation="MPIVVNLDVMMARRKMSLNELSEKVDITLANLSILKTGKAKAIR
                     FSTLESICKALDCQPGDLLEYIDK"
     gene            complement(22238..23707)
                     /locus_tag="PKM27_11200"
     CDS             complement(22238..23707)
                     /locus_tag="PKM27_11200"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNR07872.1"
                     /translation="MKNRIGIYAALTAVLFLILSFYSCKPARPEFPFREEGYWKKQGL
                     EDIILYWTQYAADREHGGFHTTLDRQWKPYGDSMRYPSMVARHLFGYSAAYLMSGDPA
                     HLETAKQLKDYLLNVAWDKAHGGFYDMLTVSGEAARANKNIFVQVYSITGLVMYYFIT
                     LDPEVLKYIREANSLLETKAWDPVSGGYFDELNPDWTVVDSSKSFSSVITPVSGYLLY
                     LYLATRDEVYLGQAERIMNTVLDKMRDKDSGWILESFDRDWNYKAPPPTVTEINTGHN
                     IETAWMLLRLHLLHPNDRFEKEALSLAEAIHRSGLDTGSGVWFASHAKDHSGGHADFS
                     YWWIQEYGMMFDLCLFKGTGREEYLSLFDLGAAFWDKFYIDREYGDTYLGVFLDGRIK
                     DGTKANRFKASYHNMETSLLNYLYTALWVNRRPVQLHFKITAARTGDLLYPLPIEDLD
                     LIIKSVHLDGKVYTETDPALKAVRLPELKEGRVIVEIGK"
     gene            complement(23697..24635)
                     /locus_tag="PKM27_11205"
     CDS             complement(23697..24635)
                     /locus_tag="PKM27_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNR07873.1"
                     /translation="MQRRKFIRNSSIGLASGLLLPALPESSPRPRKPLVRLAHLSDIH
                     VKPGIVPETGLAKALQHVQQLKPKVDFIINGGDAIMDALEADKQKTQIQFDLFKTILK
                     NENSLPVYHCIGNHDTWGWFIKNKALEDQDRLYGKTWVVETFGMPGRHYRFTKGRWHF
                     IILDSTQLNPEGGYIGKLDEPQFEWLQSELSGIPKEEYVCLVSHIPILSICAGLFFNK
                     TESNGDLKIQRNLWHTDFLRLKKLFASHPQIKTCISGHIHLQDDLTYLGIRYLCDGAV
                     SGNWWNGSFQEFSPAYAVLEFYSDGTCSRQMVQYEK"
     gene            complement(24786..26450)
                     /locus_tag="PKM27_11210"
     CDS             complement(24786..26450)
                     /locus_tag="PKM27_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR07874.1"
                     /translation="MPRISIGIIFLLLALGAKAQVPTGEIKGPYKWISKIYPGTERNY
                     WLYIPAQYDSRKAACSMIVQDGLGRANGWRLSAHLDTLIHRGEIPVIIGIYIDHGRVI
                     SADTSHYPRFNRSFEYDGLGDRYARFLMEEIIPEVKKSYNLSDDPNDRSIAGASSGAI
                     CAFNAAWERPDAFRRVLSTIGTYVGLRGADETATLVRKTEPKPLRIFLEDGYHDLNIY
                     AGDWYIANQNLLSALTWAGYEVNHAWGEGGHNNTHTLTVIADALRWLWKDYPAPVTSP
                     IDRYRGMSLTKNLGEWSELPVSKDGLNQIAAAPDGTVYFTGTDGTSIYTVSFDPATIF
                     KRIVQGHINDLTFDASGHPIYSDAGAGAIYIEQNNKASTLIRSAKPGQVLSTSKGLYF
                     MNGLQLGYYHYETKKTILHPLKSGVSGMAITSEKSYLNLIYPDEVFGYSYKINPDGSL
                     SHGQEYIHYHIPYGEKTASPHGLATDTANLLYTGTNMGVQVSDQLGRVNLILPAPNGP
                     VTDLCFGGKEFNRLFIVSGGNAYFRILNTKGNHPTAPPAKPPRPGM"
     assembly_gap    26493..26592
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26664..27197)
                     /locus_tag="PKM27_11215"
     CDS             complement(26664..27197)
                     /locus_tag="PKM27_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07875.1"
                     /translation="MNIFIRLFCLPIFLLFSMAAFAVHPASPLTETKTATLTVNQFLS
                     LDPGLISRKNGHEQAWIKRKLFRYAQHRLNKKVAQGRIQGNDALFQTGLKAEKPNRNG
                     MWSLILGGAGFLFLFASPLSLLSLPLAVGGFIFGLKGLKKDKNPLMAILGIVFCVLIA
                     LVFVLAIVFIATYFSWF"
     gene            complement(27308..28375)
                     /gene="rsgA"
                     /locus_tag="PKM27_11220"
     CDS             complement(27308..28375)
                     /gene="rsgA"
                     /locus_tag="PKM27_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNR07876.1"
                     /translation="MTLEQLGYPPSLDPYRVENQLDQFEAGRIIEEHKERYLVATARG
                     EYEAEITGHLRFSATGREDFPAVGDWVALSIHDPDFALIHAILPRTSVIKRQAVGRSG
                     ESQIIATNIDGAFLVQAVDRDFNINRLERYLTLCHESGVKPMIVLTKTDLAQPSRTGE
                     ILQEIKTRVRDVPVIAISNETREGYAALESLMVKGKTYCLLGSSGVGKSTLLNNLSGR
                     TVMKTGAISESSQRGKHVTSHRELILLDNGAILIDNPGMREVGVADSSSGLETTFDHI
                     LSLARDCKFKDCSHTGETGCAVIKAVEQGDIDQASYENYLKMEREKAHYESTLAERRR
                     KERQWGKMFKNYKKEIKKKDQ"
BASE COUNT         7126 a         7201 c         7294 g         6944 t
ORIGIN      
        1 agaccggggg caatacctgg agtacaggag ctaccaccga cagcatatat gtgaaaacgg
       61 cagggtctta tacggtaatc tacgatgacg gcaccggatg cgggaccacc ctctccgata
      121 cggtaaacat tacggttttc acacccccgg tggtatccat cagcggcacc ctgggcattt
      181 gtcccaatga aaccaccgtt ttgcatgcgg gttctggttt tgccagttat ttatggtcca
      241 ccaatgaaac tgcggattct atcgtagtgg gtaatccagg gctttacatt gtggtcgtca
      301 cagatgccaa cggttgtgtt ggatccgata cggcatccac ccaattcagc ccggtaccgg
      361 ccccttccat caccggaacc tttctttttt gtcccgggga ttccacctta ctggatgccg
      421 gaacggggtt cagcagttat ttatggtcta ccggtgcgag cactcaaacc ataacggtca
      481 gtaccgaagg tgggttttcg gtaacggtaa ccaattcaga cggatgccag ggaagcacca
      541 ccgaattcac ctcattgtac ccttcccctt ttgccgtgat taacggatac gacggattct
      601 gtcctgcaga ttccgtttta ttatccggga gcgggggcca gagttattta tggtcaaacg
      661 gcagtaccac tcctgatata tatgccaaga cggctggact ttacatcctt acggtgacgg
      721 atgccaatgg atgtaaagac agtatttcca aagatatcgt agagtttact ccgcccaatc
      781 cttttatttc cgggaccttg tccttctgcg ccggaggaag tgtgacggca ctggacgccg
      841 gaccgaattt taacagctat ttgtggtcta ccggagaatc cagttcatcc atactggttg
      901 acagggtagg aacttatgat gtaaccgtta cggataacaa cggttgccat ggatcaacca
      961 gcgccacggt cacccaggaa ggcggaatac cggatgttcc cggcccgatc acgggtagcc
     1021 tcttcggcat gtgcaacaac ctctcgccaa gcacctactc ggtcagcccc gtacccaatt
     1081 ctacctgtta cctctggaaa atgcctccgg gggcaaccat tgtatccgga ttcatgccgg
     1141 attccaattt gtttcataat gtcattcagg tggtttttga taattctttt accgggggat
     1201 atatcgaagt ggcttcccat aacgattgcg gggccagccc ttcgttcaac ggtcgcagca
     1261 tctacatatc ggcggctccc gggagcgtgc catcgaacat aacgggtcag gcctccggcg
     1321 tatgcaagca agtttccacc atgtattcca tttcaccggt caccggagcc gtatcgtatg
     1381 aatggatcgt tcccctggga gcggaaataa ccggatataa tggcgattca accgccatcg
     1441 aggtattttt tgcgacctct taccggaccg gggatatttg tgtccggtat acaacggatt
     1501 gcgggaccag tccttacaag tgcctgaacg tgtcgcctca gcccaaaatc cagtccaaca
     1561 tagccgggcc aacaacggtc tgcgcccaaa tgcaaaacgc aatgtttaat attccgcccg
     1621 tgatcggcgc taccgcctac ctatggtccg taccgaccgg cgcaaccatc gtttccggcc
     1681 agggtaccac acagatctcg gtgaactttg gaaaccatac cggtatcgtc accgtaaaag
     1741 cgataaacga ttgcggcaac agcctcatcc agtatctttc ggtcaatgtt accaactgca
     1801 cccgtttccc atcccgcgaa ataaactata ccaaacgcag accgaacgac tttaaattcg
     1861 aactgtatcc gaatccttcg gatggactct ttctgctgga gctaacctct ccggagccgg
     1921 gaacggaata tgccctgacg gtgtctgacg ccctgggtaa aaaagtgctt gaatataaat
     1981 taccgggaaa ccagtttgta cgggactggc tggacctccg tcatctgggc aaaggcatgt
     2041 attttatgac cattgcaaat aaatcccagc agcaaacggc caaattggtg atccattgat
     2101 ccggtcaact tttgaataga aaataaaaaa caaggacaag cacggactgc aacaacaggg
     2161 tcccgtccag gatgccgaga tgataaaaag gcaatacctg caatttttca ctggctaaga
     2221 aaaaggccgt ggtcagcgcg gaaatcagca gggcgacggt gatatagctc atgcccatcc
     2281 cgggatattg aacgagggca atggccgcga acattgccag gcagagataa gaaaggacca
     2341 ggggattcgt tttcttaaaa agcatgggaa tcgtctttaa acccactttg cggtctgcct
     2401 ccaggtcgcg gatatcaaac ggcagggtga tggcaaggat aaaaaaaatt ctttccacaa
     2461 gaagcgccgc caggaaggga acctggtaac tccttccgct gtggatcacc gggagcagga
     2521 tggtgacccc cgaccaaacc ccggcgataa gaaaaatttt gaggtaggga atctcgcgaa
     2581 gccggaacca ttttttcccc aggtgccgga caggggtcga ataaaaaagg gccagtgcgg
     2641 caagcggggc cagtgcaatc aacacttctt ttttggcctg gaacaaggtg atcacaaaac
     2701 ccgtcacgga aagaaacacc atcaggtaaa atccgggctt atgttgccgg agccattcgt
     2761 atttttcatc ttccagggct tccggcacat atacgacggt gatcagccgg tgcaaattgt
     2821 attcgaacag ggtggcaaaa aagatcagga agaggtaagg gtggacatgg ggcctgagcc
     2881 ctaactgcac ctgggtttcc agggcgaggg ccaccgcgca taacgcaatc agtatattgg
     2941 aatggataag ggctgaaatc caacttttaa tcacttctat ttgtttaccc ccggtttcat
     3001 tccggaccgc tcttcgtagt caagttcttc cttagtcctg gtccggtatc ccgcatccag
     3061 gattccgttt tccagcctga gcacgccccg cggacaaact gcggaacaaa taccgcagcc
     3121 cacgcaagat gccctcacga catcctgtcc ccgctgggca taggatttta catcgatccc
     3181 catctcgcaa taggtagaac aattgccgca ggaaatgcat tggcctccgt tgactgtgat
     3241 ccggaaccgg gacttggttt tttgcaggat acccaggtaa gccgccatcg gacatccgaa
     3301 gcggcaccat acccggcttc ccatcagggg ataaaagcct accccgatca cccctgaaaa
     3361 ggccgcgccg ataaaaaacc cgtaccaggc actcaattgg taactgttta aaaacaggat
     3421 attggctgcg ccggtaaacc gggtgtacaa aaccatagcc gtcatcacca ccacaaaaac
     3481 caggaccccg tggaccatcc atcgttcgac tttccaggcc ttcagggaag aggaggacag
     3541 atgccggaac ggatcaccag ccgtctcggc caggccgccg cagccgcaga cccaggagca
     3601 ataccatctt ttaccgtaaa accaggtcaa cacaggagtc aggaccagga aggaaacgat
     3661 accccagaac aacataaaat aaccgagcat cccgctttga atcaaaccat cgatatgcca
     3721 gtcaaaaaag aaataataat tcaggggcca gatgttttta aaatcaaaat aaggccggtt
     3781 gaggccttgc aggatctctg gaagaataaa ggcgaaagcc gtctggaaaa aaagtaccga
     3841 taacgtgcgc aaaacctggt accggtcatg cctgtattta tagatgaact tgatccccat
     3901 caggaacatg atcagggtgt agaaggtgcc ataaacaaac cactggctgg ccggtttccc
     3961 ttttaagtat tggctcaggg gatcaaacag acggatcagg cctgagtttg ctcccccctc
     4021 tccctgtccc tgtcccaggt attgaggata ccagtacagt agaatataaa atccggtgag
     4081 tattaccgcc aataaccagg cgaggcttcc ccttgcggtc aggctcctga accagacccc
     4141 gttgtttttt atacctgggg gatgtgcatg ataggtttcg taggaatacc agaccgaacc
     4201 ggcactgacc aggaggattg aggtccacaa ccaaacgctt gcattttggg ttttgaatga
     4261 gatccagtaa atgagttgga tcagtatccc ggaacccact aatcccagcg cccctttttg
     4321 tacagggctc aggtttgcgg aatggactcc ggcagagagg gacatggatt cggcgttctt
     4381 catcctgtca ttcatttatt ttatttaaaa atcgggtgac cgcatcccgg tcgcgcccag
     4441 acttcaaaat cagtcttttg ccggattgga tatagtacag atccatcaac cggctttcat
     4501 actgggggaa aaactcggga tcaaaattgg caaggccgag ttgaggcagg acggtctcta
     4561 tggtagcacg tgaacggatc cactgttcac agacctcctg cctgtaccgg atgcccatga
     4621 ggcagaaacc gcggacggcc agcgggaaat ttggactagt ggcgctgttg taattgatcc
     4681 ggatggactt ttttccattg gcgccctgcc aatacaggga ttggacatgc tccaccggtt
     4741 ggggttctat ttcgccgtaa acctggtatt cgatatcgaa aaatttcgca gaattgtacc
     4801 agatgcccgg gtcgtatttc acaggaccct tgcaaagggt gtgcgctacc gtctctccca
     4861 tgatccggcc ggtataccag atcgcttcta tggcttttct gccgggccgg ggggtccaga
     4921 gctccgcgca atccccgatc gcgtaaatat ctttttggga agaccggagg taatcatcca
     4981 ccatgacgcc catattggtt tcgatatcgc tgttgtataa aaaaccaata ttggggcgga
     5041 ctcccacggt aagccctaca aactgggctt caaggtggtc atcgtgcccc gtgcgcaccc
     5101 cggcgaccca tccccttcca tcgttgtata tttcttcaag accggtattg agccggagat
     5161 cgactccatg tctacggatc tcttcattga ccatggccga ttcttcggcc ggtaaaaccg
     5221 tattccagaa actggattca atggcaagaa aactgaccgc gatatttctg gatcgtaaca
     5281 tttcagccag ttcgaccccg atcagccccc ctcctacgat gacggctttc ctgatccgcc
     5341 cggtctgatc ttccagttgc tgtaaatccc gaagatgata aaggccctga accccttgga
     5401 gatcctgtcc tggccagtca aattttttgg gcagggaacc gcaggccagg atcaggatgt
     5461 cataagacag cgattgtcca ttgtgcagtt gaacggattt ggatacagga ttgatttcgg
     5521 taacccgtcc atgcagccgg tcaatgcggt ttttagccca aaaaccgtct tcatacaatt
     5581 tggtgtgctc aagacgaagg tgccccatat acacatacat cagggcggta cgcgaaaaag
     5641 gatattcgga ttcttcggag atcatcagga tctcatgatc gctgtacttc cggataaaac
     5701 gcgctgccgt acttcccgca atcccgtttc cgatgatgat gatcctcatg attctgtatt
     5761 taaacgtaca gaatattcat gagaaacaaa ggaggctccg ttaaaacagg ggctggtacc
     5821 tcggtttcca gaggcggtca taggtaatgc ctacccttaa aagcatactc atgccggtct
     5881 ccctcgtctc ctcaaccttt cctttgaaga agaaaaaatc ggaggccacg gagagggaaa
     5941 gttcatcgat cagcgggatc agcagttcat ggaccatatt ataccgcagg gccaggtcgc
     6001 ggttggtgtc cgactttgct ttaaaaaacc aggtcgcatc gttgcgtagg gaatagatca
     6061 ccgccccccc gcccaggaag aatcggccat ccgcccgggc ggtaaacccg aactgggcct
     6121 tattttgtcc aaaatcctgt tcgagcacgg aggcgagttc cacattgcgc cagttgcgat
     6181 ggaagcggcg ggcgaggccg attactccac gaagggcctg ctgtttgtaa ttttcctcca
     6241 aagtggctgg attgatggtg ggtgtaaatt cggtatcgta ctggccgcgt acaaagggtt
     6301 gaaagatccg gtttgacttt ctccggtagg tgaggtccag tttaatatcg tccgccgatt
     6361 cggagatttg ctggatattt ttggagattt ctgaattttg tttggcgtat gccagcgtaa
     6421 gcccgaggtc cagggcgttt ttaaggccat ccatggttgc cttaaaccgg ccgctcgcgc
     6481 cgatcaccca ggaattggtg gccgtgatcc tgctttccgg gaccgccccg tatccatcgc
     6541 tgttgtattt ccggttgtaa gaagtccaaa gggtgggccg gtcaaaatca aacgaaagca
     6601 agggttcaaa ccggggatct ggtttcaggc gttcggtgac ggcttgaaga aacttatccc
     6661 ctttgttgtt tttattaagc cgtttaagct cattgagtac aaaactccgg agtgataact
     6721 tgtccccctc cgggctttcg accaaccggc cttctttatc cataataaat tgacggcgga
     6781 ccctgcgtga ttccagaaaa tccgcagccc tgaccccttc gaagatcaca tcggtggtgg
     6841 ctaccctgta aaaaccatcc ggatcaaccg gcctcccgag aattctgcaa ttgagctgca
     6901 gccattccat ggataccgaa ggcaaaccac gggagtaggt ttgttgaggg ccgggtttgg
     6961 gacgggtcag cccactgaaa atgaggtcct cgtgtacatc gtctgccagg atcttaagaa
     7021 gggcgcttcc cttgacatca caaaatagaa tttcatcctc attccaaagc caggcactca
     7081 cttcccgctc atgcaatttg ccgatgaggg gcgggaaata agggaatttc cggattacgg
     7141 ccagctcggt gggtccggca ttccgcaata aacgggcgac aaattcctcc cacatccgtt
     7201 gggataccct tccctgttgg gtggtggcat cgtatttttt aagatcgggg taaagggtta
     7261 ccaggtcatt gaatgcaggg aaaagaaggt cgcccttggg tttgctgacc cgttgggaaa
     7321 gggaaagaat ttgccggatg accgaagtat caggcactgt ctggtccgtc accgggtcca
     7381 gccggtgttg aaggccgagc aatctgcctt cagcatcaaa tttcatccgt aaacgcccta
     7441 cccccaatcc atcggcaaat gaccgggtca ccagggcagg ggatcccggc cggttgaggt
     7501 tgtggccagg taattccacg gattggatca cagtttcagg gctcaggcgc gaatgcaggt
     7561 ccgccacgat cgcatcgatt cccgctactt tttccgcaag ggcggcgttg tcccccgcat
     7621 cgagatttga taaaaccacg atagcctcta cccctgattt tctcatctgg ttaacttcat
     7681 aacgaatggt ttccagatag ggtaagaact gaaaatcttt gaggctgttt ttggaaaggt
     7741 ccccctggag ggtggggtcc acaagaccca gcaggcctac ccggatattg ccaaacggga
     7801 ggatcaaccg gctatggaac aaagaactgt cccggctgcg gatattggtg gccagcagcc
     7861 ggatccccgg aaagtccagg gaaaggcgct tcaaactgtc tgcccccaga gataattcaa
     7921 aatgataggg gaccaaaatg gaatatccaa gcttggtgag aaggctaaaa tcaattcttg
     7981 cccgctcgag gtcgtcgagt ccaaaatttc cggtgcggtg gccaaggtcg gctaaaattt
     8041 ggtattcccc gttttgatca gcattaaccc attgtttttt aatcaccgta gatctcacgg
     8101 ccatttcccc caggttgaag ggcaattcat acaaacggcc ggtatcgccg atatccatcc
     8161 ggttgacttc cccaatggtc caaagatcag gatgctccgg ccagacggct ccttcttctt
     8221 caatgataat tgccgggtcc ccgttgcgga tggtcatgga ataataggtg acttgggttt
     8281 cagccaggtc gggaatatcg gttttttgcc ttgggttttt tttcatcatg tccagcatgt
     8341 cgggatttcg acggaggggg tcctggacta acaggctgtt gtgggttttg aacgcaggat
     8401 attttttaag ggcttttgcg gaatcttttt gggctaaaat ccgcggaagt tcattgcccc
     8461 tgggttcgtc ggagggcagg aaaacggcca acccgggtgc gcgccagcag gagtggctga
     8521 ccagtttaaa aggtttatgt tcggtgttgg cttgttcggt gaccagttca tgttcctcct
     8581 gggatcgcat caccccgagg gcccccaggc tgcggccggt gtaaatgatg gatataccgg
     8641 tggtatctgc ggtaccgggc aaggcaataa gttgcagttt ccataccagg aagaaaaaac
     8701 aaatgaccag ccgcattttg gcaatttgac cgatccggcc cagggagaca gatcagggta
     8761 aagatagtca aaaggaaaag cattataagg gatggttatc ccttaatcat ttactattgc
     8821 gtatcgcaac atcaccagtc cggaagggta tttccggctg cttttcaggt ccagcctgac
     8881 ccggtgtttt aatccgtgaa acaagggctt gccatcaccc aggaggatgg gatggacagc
     8941 gaggaccagt tcatccacca gccaatgatc cataaaggaa gtggtgaggc tcgcaccgcc
     9001 ccacaaccag atgtccttgc ctttttcttg tttgtattgt ttggtccagg ataccgtatc
     9061 ccctgaaacc agatgaaaag gctcctgcac ttcatcttct ttcagcgaat tggaaaacac
     9121 atactgtttg atcttcgaca tttttaccat aaaaggatct tccgccgcaa cgggcatgga
     9181 ttttgccaaa tcgtaggatt tacggcccat aaagagggta tccacctttt taagaaagga
     9241 ggacatccca taatcctggt cggtgaggca ccagtcgtac tccccgttgg ggccttcaat
     9301 gaaaccgtcc aggctgacgg ccagttgcag tatgattttg cgcatagttc aaaacaaaga
     9361 ttaattaaat ttacagtttt actgcctagt ttaatcagat ttgatgagga aatcgctatc
     9421 caaactaccc ctggtaggca ccaatatatt taccaaaatg agcggcctgg cggccgaggt
     9481 gggggctatc aatattgcgc agggatttcc cgatttcgcc tgcaatccgg agctgatcgc
     9541 gctggtcgat caggccatga aaggaggctt taatcagtac gcgcccatgc ccggtttgct
     9601 aaaattgcgc gaagggatag cggaaaaaac gtttgaactt taccggcggg aatacgaccc
     9661 gggtaccgaa atcaccgtca tgccgggagc caccgcaggg atctattcgg ccatccaggc
     9721 cctcgtccaa ccgggggagg aagtgattgt gctggagccc tgttacgaca gttatattcc
     9781 atccatcata ctggccggcg cggtgccggt aaggtataaa atgaaatttc cgggttatgc
     9841 catcgactgg gaggaagtcc gccggctgat taattccagg acccggatga tggtgatcaa
     9901 tacgccgcac aatcctaccg ggaatatcct tacccgggag gatatgctgg ccctgcaatc
     9961 cctggtcagg gataccgacc tcctcgtcct cagcgatgag gtctatgagc acatagtctt
    10021 cgacggggaa aaacactggt cggtatccgc ttttccggaa ctggcggaac gaagcatcat
    10081 ggtggcttct tttggaaaaa cctatcatgc cacgggatgg agggttggtt atgtattggc
    10141 tccggctgag atgaccgcag aaatccgtaa ggtgtaccag tttatcgcct tctccgcatc
    10201 aacgccggtg cagcacgctt tggcggcttt tttgaataaa aaggaggcct accttcaatt
    10261 gaatcttttt tttgagaaaa agcgggaccg gttcagggag atgatcagcg gcagccggtt
    10321 cagcctgctc ccttcccgcg gctcttactt ccaatgcgcg acttacgaac ggatcaccga
    10381 tgaaggcgat gccgaactgg ccatccgcct tacgcacgaa tacaaggtgg ctaccattcc
    10441 cgtatccgcc ttttaccagg atcataccga ccaccgggtt ttgcgttttt gttttggaaa
    10501 aaaagacgaa acccttgaaa aagcagcaga gattttatgc aagatctaag cctcaccctc
    10561 ctgcaaacca gcctgcaatg ggaaaatccg gaagccaacc tggcccattt tgaccgcctg
    10621 atttcccgga tccaggggcc gaccgatctc atcctgcttc ctgagatgtt caataccggt
    10681 ttcagtatgc gggcggacag cctggcggaa accatggagg gacccaccgt aaactggatg
    10741 aaaaccaagg cggcacaaaa tcagacggtg gtctttggca gcctcatcat ccgggaggag
    10801 aaggccttct acaaccgtct ggtggcggcg cttccggatg gttctttgca atattataac
    10861 aaaaggcatt tgttccgcat ggccggggaa caattgactt acaccggagg gaatgaactg
    10921 ctgaccctgg aaataaaagg ctggaagatc aggccttttg tttgttatga tctccgtttt
    10981 cccgtatggt ccaggaattc gtttgatccc cttgccggct ggcaatatga tctggccgtt
    11041 tatgtagcca actggccgtc cgcaagggcc catgcctgga aaagcctgcc ggtggccaga
    11101 gccatcgaaa accaggcctg gatcgccacc ctcaaccgca ttggcagcga cgggaaaggc
    11161 catacttaca gcggggacag ccaggtgatc aattcttatg gcgaaacggt ggttcacctt
    11221 gatgaggcgg aatatgtcca aaccgtggtc cttgaccgca acagccttga aaaacaccgc
    11281 gaggtattcc cggtgggccg ggatgcggac cgtttcgaga ttttcccctg aagtgatatg
    11341 aatctaactc tccgccggga aaaggcctca gattttcatg ggatttacct ggtccatcag
    11401 gccgccttcc gccaggaagc cgaaggcaga ttggttgaaa aactcaggaa aaacaaagcg
    11461 tatatcccgg aattgtccct ggtagccata caggacaacc ttgtcgtggg ccatatcatt
    11521 ttttcaaaaa taaaaatctc cgggggacag aaggattatg aaagcttaac cctggctccc
    11581 ttgggcgttc tgacggaatt ccagaataag ggcattggca gtcttttggt tcgggaggga
    11641 ctgaagaaag cccgtgaact gggctaccgt tccgtcatag tactgggcca taagaacttt
    11701 tatccccgat ttggttttgt tcccgcatcc agatggggaa ttgaatcacc ctacgaagtt
    11761 ccggatgaag cattcatggc catcgaactg gtgcccggcg gcctggatgg gataagcggg
    11821 aaagtgaaat acgatgaagc atttgaagaa ctgtaaaggc ggtttcattt tactcgttaa
    11881 gtcgcttttc agagaagatc cggtaacttt gtataaaaaa gcatgaaagc aaggaacgcc
    11941 atcggactgg acagcaaaaa gtcaaaacaa ctggctgaaa aactgaacga tttgctggcc
    12001 cactattcca tattttatca aaacaccagg gggtatcact ggaacatcaa aggagagaag
    12061 ttttttgaat tgcatcttaa gtttgaagaa ttatacaacg acctgctcct gaaaatcgat
    12121 gaggtcgccg agcggatcct caccctggga tatgcgcccc tgcacaatta ttcagaatat
    12181 aaaaaggctt cagccatcaa ggagagcagc caggtttcgg atggccttaa ggcggtggag
    12241 gatatcctga actctttccg ggtgatcatt ggcctgcaac gtgaattgct gggcctgagc
    12301 gccgaagccg gcgacgaagg gaccaatgcc ctgatgagcg attatatccg tgcccaggaa
    12361 aagctggtgt ggatgtattc cgcctttctg aataaatagg cctccttccg gtcaagccgg
    12421 ggtgcgactc tattaaatga aggcgtataa aaaatatttt gaccaagcgc tccggctgaa
    12481 ttatcccggc caggccgtgg actatatccg ggaaaccgaa ttcttgtatc aagggatccg
    12541 ggcggatctt ggtttcgttt ccagctccgg aaaccccatg gaccggcggt tgtatatttc
    12601 aggttatttt ctcgcactca ttcaagtcct ggaccgggat ggaaaaagca tggaggaaat
    12661 caggagcctg gtcaccggca tcgcctccga atatgtacgg cccaaaaaca aatggcaggc
    12721 ttttttaaaa aggctgccgg ccaggctgat caaatcggga ctagcttcca tcctgttaaa
    12781 aaaaatggac cggatggtca gccgcaatgc ccatcccgaa ggcttccggg tcagggtcct
    12841 caccggcaaa gaagaaaccc tgggctttgg atacggagtg gatatcctcg aatgcggaat
    12901 ctgtaaactt tttgcccggc acggcttttc aaaatatgcc gccttgctct gtgaagtaga
    12961 ctacatcact tcttccctgg ccggattgca attggtgagg accgggacca tcgcgaatgg
    13021 agcggaacag tgcgacttca ggttcaaaag gaccggcggc tgaggtcttc ccagcaagtc
    13081 tacaaatacg aagtaaaacc ctctgccgtc agacctaccc gtccgaagcg ctgaccggtc
    13141 acagtctggt tccgaagggt ttcgggaccg gtccccaggt tttgtttatc cttgagtcgg
    13201 gggttcttgc aggcagaaga cctaaaaaat aaaagtaatt aaatactttt attttaaaaa
    13261 aaaccctata tttgtagggt catctaaaac aaaacatatg gaaaccgtaa ctgataatat
    13321 gcttgatgtc accctcctgg aaccccggat gaaacacccc acgatcttcg ccggcttcga
    13381 cgcgctggcg gaaggccaaa gtctggtcat ccacaacgat catgacccca aaccgctgta
    13441 ttaccagctg ctgggggaaa ggggcaatgt gtttaactgg gaatacctga ccgaggggcc
    13501 ccgctggtgg aaagtcagga tcaccaaacg ccgggccgac gaacaggatg agaccctggg
    13561 acagatcgcg gccaaagatc tgcgcaaagc ggaagtcttt aaaaaatacg gcctggactt
    13621 ctgttgcgga ggaaaaaaga cggtaaaaca agcctgtgct gaaaaaggcc tggacgtcac
    13681 ccggatcgag caggaattgc agcaagccga taaattgcca acggcacagg cccttcctta
    13741 ccaggaatgg agcctggctt tcctctccga ttatatcatc aacactcacc acctgtatgt
    13801 aaaaaagtcc cttcccgaac tggaagccta tgccatcaag gtggccgccg tgcacggcgg
    13861 aaaccatcct gagctggcgg agatcaaggc gctggtgcag gatttaaaag cagaaatgac
    13921 gacgcatatg gccaaggagg aaaaaatcgt atttccttat atcaagaacc tggaaggggt
    13981 ggcctccagg ggagtggcgg tttttgacag catcacccag ccggtgcatc agatggaaat
    14041 cgaacatgaa tccgcagggc tcctccttga aaaaatcagg cagctgagcc acgattacca
    14101 ggctccgcag gatgcctgcg gctcttaccg catgctgtat tccatgctcg aagcctttga
    14161 aaacgacctc cacctccatg tccatcttga aaacaatatt ctgtttccca aatcgattga
    14221 actggagaaa aaatccaaat ccattcaagt ccaaagcgca tgattatggt ttatcgtcga
    14281 actgaaccgg caagtccgat ccggcggcat ccttcgatcg tccccttttc gcgtgatcat
    14341 cattttggcc tgttgctttc ctggaagatc cggcaaggca tcaaaaacca catagacggg
    14401 gaaaggatca gcgattatgt gatctatttt tttgagaaag ccctggcccc gcatttcagg
    14461 ctcgaagaag agcagttgtt tcctcttttt gcggaaggac atgccggctg ccaacaggcc
    14521 cttcgcgaac atactcaact ggaattcctg gtcaacgaat tgcgccggga taaaacgggt
    14581 aacgtcatcc tccaggcctt tgcggacctt ttggaaaaac acatccggtt tgaagaacgg
    14641 atcttgttta accagctgca aacggagatc tacgatcccc atatcaccga agaccggtca
    14701 cagcacctga ccaaggaaga ggacccggac ctgaattggg aagatcattt ctggacggcc
    14761 tccccagaaa aacgcaggag aaataaatga aacccatcaa ccccgatacc aagatcgcag
    14821 ttcttctgaa gcaggatcca agggctcttg aggccatcat cagcctttca ccccgttttg
    14881 aaaaactgag aaatcccctg cttcgcaaac tcatggcagg acgcacctcc atccagatgg
    14941 ccagcagaat cggtggggtg aacatcaggg attttttatc aaaactggaa gccctgggtt
    15001 tctacattga agggtccggt cctgtccctg cggaatcaaa aaaccgggaa acccctgact
    15061 tcctcaaaga cctccccccc gatcgcatca cggagctgga tgtcaggccg gagattgaaa
    15121 cgggtaagga ccctctccat ctgatcctgc aacgacttaa agatttaaaa ccaggacaag
    15181 tgctgaaact ggtcaacagc tttgaacctg tcccgctgat cgaattgctg caaaaacagg
    15241 gatacgaatc ccatgtcgag gagatcagcc ctgacctggt catcacctgg ttttatgcca
    15301 ccagaccggt ggacatccag ggtctggatc cggccaaaac caagggctgg gagatcctgt
    15361 tgaaacggtt tgatcaaaag atgcagaccc tcgacgtaag gcaactggaa atgcccttgc
    15421 cgatgttgac catcctggaa gccctggaga ctttgcctcc ggatcaggcc ctgtatgttt
    15481 atcacaaacg gatccctctt tttttattgc ccgaactggc tgagcggcat ttcgattacc
    15541 ggatcaaaga ggtttcggat caggaggtac atttgctcct cttcaggtca tgatatgttt
    15601 ggcgtcccgg tatcttcggt tgcccagaaa accacttcgc ataaggtggt actgcccttt
    15661 tacggctacg cagcagcatc cctgtttgca gcggcactgc tgatcctggc caattcagcg
    15721 gcctttaccg gacattattt tcatccgcgc atcctggcca tcacccatat cctggccctg
    15781 ggctgggcta ctatgatcat tttgggcgca agtcatcagt tggtacccat cctggtggaa
    15841 ggcagcttgt acagtgaaaa gctggcgatg gcttctttta tcctggcggg gctgggcatt
    15901 cccctgttgt cttatgggtt ttattatttt gaaatgggtt ggccggccaa atggggagga
    15961 aggctggtgg tcctggccat cctgtgttat ctcataaatc ttttcaaaag catttcacgg
    16021 gggaaacagg acaatgtcca tgccctcttt gtgtttacgg ccggcctttg gttgttcatt
    16081 accgcctttt tcggcctggt actggtttat aattttacct acaccctgtt gtcggaagac
    16141 actctccatt acctgcccct gcatgcccac acggggatcc tgggctggtt tttactgctg
    16201 gtcatggggg tggcttccag gctgatcccc atgttcctga tttccaaata ccgcaatccc
    16261 cgcctccttt ggcttatcta tttactgatc aatacggccc tcctcgggtt tttcttttta
    16321 tacctcagca ccgtactgat aaacctgatt tttataccgg cgctgatggt cctggccgcg
    16381 gtgtttttgt ttatctatta ttgttacagg gcttatcagg cgaggcttag aaaaagggtc
    16441 gacccccagg tccggatttc cctgttttcc gttggtctcc tcgcccttcc atggatgctc
    16501 ctcctcatcc tgatcggcct gcttttgcgg aacggtggag aacaaaccag cctggccctt
    16561 gcttacggtt tcctggtatt ttttggctgg atcacggcaa tcatcctggg catgaccttc
    16621 aaaaccctgc cgtttatagt ctggaacaaa acgtaccacc acctggccgg cattggcagg
    16681 actcccaacc cgaaggacct gttccatgac ggaattttca gggttatggt cctggcatac
    16741 ttggcaggcc ttattttttt taccgccggc atcctagcgg tccaactctt tttactgcag
    16801 gctggcgcgg ttttgctgct gggtacggcg gcattgtata actggaacgt atttaaactc
    16861 ctctttcata aaaaaccagt gacatgaatc cggtcaccaa ccttcctgaa aaatccaagc
    16921 tggcccttga ggcgctccac caggtcctgg acccggaaat cgggctaaac gtcgtggacc
    16981 tgggcctgat ctaccggctt gattttgatg aggaaaaaaa ggaattgttt tgcaccatga
    17041 ccctgaccac ttcgttttgc cccatggggg agtccatcac ctttggcgta ctgcaagccc
    17101 tggaagcctg tttccccgga tatgccgtcc atgtcgggct cagttttgat ccgccctgga
    17161 gccaggattt gatatccggg gagggacttc aatttctgaa cagatgaata atcaataatt
    17221 aagaattaat aattaagaat taaaaataaa agcagatatc atgctcagcc ttacctgtaa
    17281 agccgccata aaagccgtca tctttctggc ttcccgcctc gaggccggtc aaaaaacggg
    17341 tatccttgaa atctccagcc atattggtga aaacgagcac acagtaggca aattgttgca
    17401 aaaactggtt aaggaaaaaa tcatccacag cgccaagggt ccccgggggg gcttttacat
    17461 gaccgccggg cagcaggacc agcccgtgat ccgcgtggtg gaagccatcg acgggaaagc
    17521 cgtctttgat caatgcggcc tgggcctgag ccgttgttcg gccacccatc cctgtcctat
    17581 tcacgatgat tacaaagtcg tgcgggatgc atttaaaaag atgtgtgagg agagaaaaat
    17641 ccgtgatttg tacgggccgg tgcaccgggg gacggcgtac ctgttgggat gaagtgaagg
    17701 gttgacccca aagtcaaccc ttcactccaa taggtgcatt tctttctatt tttgtccaat
    17761 gtcccgcatc agctccttct ccgcccctca caaagggtta cggaaccttc tttccaaatt
    17821 ttcatttctc gccggcaaaa ccaatttcga agatcccgcc caggttgcag atcttaaaaa
    17881 gctgggattt gacctgttcc gactgctgga tgaacatgcc aggcttgaaa acgaatttat
    17941 cctggccacc ctggccgaaa gggtacccca tggcgccgac catgacttgc gcgaccatga
    18001 atacctggaa gaggaacaag acaaggtctc ccacaagctg aagcgcctga atggcaaaca
    18061 ggatgcggat gccggccacg atttttatct ttccttttcc aaataccaca gccgttacct
    18121 tgcgcatatt ctgcaggaag agacggtcac cgaaaaatta ttacaggaaa atttttcgga
    18181 tgaggaattg ctccaattgc gtggccgtat catgaaaagt attcctttct cggatttttt
    18241 aatctgggtc aagtacatca tcccggctca aaatgaatct gaaaacatag gtatgcttaa
    18301 gggatttatt gcaagcgcac caccggaggc ttttcagaaa gtgagtgagg tgctcaggaa
    18361 ggaattgcct gaagcagagt attcgtcttt aattcaaaaa gttacggggt agtcaggcgt
    18421 tgatcctcct gccgcattac tcaataaatg tttgcccatc tcccgtttcc acttctgggg
    18481 tttgggttta aatttcggga cgcccttctt ccgcactgct atctttgcga aaatgatttc
    18541 cacccccgac cagcgcgtcc gcgtgtttgt aagcagtacc ctggaagaac tggctgaaga
    18601 aaggaaggtc gtccggaaag ccattgaaga actgcgcctg atcccggtga tgtttgaatt
    18661 gggagccagg gcccaccccc ccagacagtt gtaccaggcc tacctggagc agagcgatgt
    18721 atttattggc atctactgga accaatacgg ttggactgcc ccggaaatgg acatttccgg
    18781 cctggaggac gaataccgga tgtccgggtc taaacccagg ctgatctatg tgaaatcttc
    18841 gacgtccagg gatcccaggc tggaccggtt gatccgggat attgaaaaac aggggagcct
    18901 ggcttataaa ttgtttcagg acctgaggga attgcaggag tatattgaaa atgacctggc
    18961 tgccttgctt tcggaaaaat tccggagtga aacccaaaag gaaagcccgg atcccaaaat
    19021 attttttaac aatattcctc aaccatacca tgaactgatt ggccggcagg aagaaaaaaa
    19081 gaatttggaa gacttgctcc tcggccagca taaaaggctg attaccctga ccggaatggg
    19141 agggaccggc aagtcaagac tggctattga gattggtttt gcagccctgg cccattttga
    19201 gcatggggtc tgttttgtaa gcctgggctc catccgggat cacgaccagt tggagtcctg
    19261 cattgccctg gccctgggcc tttccaatgc ttcctcccag gccctgcggg aaaccctgct
    19321 ctcctatctt tcagataaac ggctcctgct tatcctggac aattttgaac atgtcctgga
    19381 gggggcaggg ctcctgagcg atatcctgag ccgaacccgc caggtatcca tcctggtgac
    19441 cagtcgctcc tcccttaacc tgagggcgga atggattttt ccgctttctc cgctgcccgt
    19501 ttccagtaag gtggaatgcg gggaatccgg gaatattgaa ctcccttcct taaaattatt
    19561 catggaacgc gccaacagcg ttaatccccg gatcaactgg aatacagaca acctggaagc
    19621 ggcggcggtc atttgtttcc gcctgggagg cctgcccctg gcgatagaac tggcggccat
    19681 gcggaccaaa tacaacagcc cggtgcaatt gctcaaaggc atggaaaaat cgctggacct
    19741 tctcaccgga ggggcccgcg accttccggg ccgccaacag tcttttcggg ccactctcga
    19801 ctggagttat cgcttattga gccccgagga ccaggaaatt ttcaggatca tgtccatttt
    19861 tcaggatggt tggtacgcgg ataccctcag gtccgtcagc gggcttcctg ccgaaacttg
    19921 cgccgcggct accgagcgcc tgctggacgc cgggctgatc cgcctgaaaa acattgatcc
    19981 ggaaccccgg ttcgacatgc tgcagcccat cgacgaatat gccggagagt tgtttgaatc
    20041 ggatatggaa aaggacccgg tccgggaccg gttcctgcaa ttttatgtgg atctggcgaa
    20101 ggacatcagt cccagcctgc ttacggtcaa ttacaacagg aactggctgc ccatgctgag
    20161 gaccgattat gaaaatttca gggaggcctt tcaaattgcc cttttccgcc aggataaaaa
    20221 aagcgcggcc accctgatca atgcggtggg cacctactgg atgtttgaag gccatcaaaa
    20281 cgaaggactg ctttggctca gccaggcggg cctcaacgaa cccggagatt ggcggaccct
    20341 gtccaaagag gaggtccatg tctacgcaaa cagcatgctc tgttccggta tttttctttt
    20401 tttccccgcc cggttcgaag aagccatccg ggacctgaag attggccggg atatgtttga
    20461 aacgattcag gattaccggg ggatgtcccg gtgcctgact tacctgggcc tcatctccct
    20521 ttctaccggg gacctggaag ggaaaagtta ttttaatgaa gccatccgca ggggcaatga
    20581 gagcggggat tatttttcgg tttttatttc caccaccttc ctttcggaat attatttact
    20641 caccggggat tcggcccagg ctatccagca gatcggcgag atcgatttcc tgcttaatca
    20701 acggtatact ggcccaaact atgcggtcat gcaatcttac cagccgatct tttacgtgga
    20761 aaaaggaaat gttttccttt atgccggaga cctggaaacg gcccggcagg cctttgagga
    20821 aagcatccgg ttgtttgacc aaagcggcct caaaaccagc aaaggctacg cattcgtggg
    20881 gctggcgatc gtgcatgggc tccggggaga ctacgcccat gcgaagaaga caagcctgag
    20941 gggcctcagt accggacgtg aactgggaga tgtcatcatc atcctggtca acctgaaggt
    21001 ctttctgagc agcctgatcg gggaaggaga gtggaaatcg agcctgcctt tgctggatga
    21061 ggtatttgct tttatccgtc attataaata tgcctcctgg acgggggaca agatcaccga
    21121 tgcgtggata cagggattat tggaccagaa acagttaaaa actaatcctg gaacagatgt
    21181 accaaagata accctggatg aactgataca aaaaaccttg tcattggcaa cagaggggta
    21241 agatcagttc cttcgctcga aagccattga ttattaaaag ttcaggtgtt gaaatccgaa
    21301 cccttccttc taattgggtt tgatggcata aggccttaat acaagtcaat caaaaaatat
    21361 ttattgataa tcaatgcaaa attattgatt atcaataaat atttatttat atttgataat
    21421 tctaaaaaat aatataaact aaggtgtata tggaagtcaa aacaaattcg aggacaggtc
    21481 aaattcttga ttttatgtac attctggcct gggtggcctt cattggattg atgatagaag
    21541 ccggcgcgat cctgatttca tatggggtaa gttgtgtaaa cccggaagcc gccaggaatc
    21601 tgtacaaggg aatggacctg taccccctca gacattacag tttctggcat tatacccagt
    21661 ccgtttcctt aatatttgcc ttatcggcca tgaaatcatt catattgttt ctggtcatca
    21721 aaacactttc gaaggtaaac ctgaagagcc cctttaccat cgaggtcgca aggacccttg
    21781 aaaagatcag ttatgttttt ttgggcacct ggattattgc aataatcaac aatgcccaaa
    21841 atgaagggtt gctgaaaaga gcggggattt ttattgagga tcgggatgcc ggggaattcc
    21901 tttttatggc cgggcttgta tttattattt cacaagtttt caagcgtgga gtagagcttc
    21961 aatccgaaaa cgaactaacc gtataagata tgcccattgt cgtaaacctg gatgtgatga
    22021 tggccaggcg taaaatgtct ctaaacgaac tttccgaaaa agtggatatc acgctggcta
    22081 atctatccat actcaaaacc ggtaaagcca aagccatccg atttagtacc ctggagtcta
    22141 tttgtaaagc actggattgt cagccaggag atttattgga gtatatagat aaataaacta
    22201 tcgtgtgaaa gcgtaaactc ctggttcccg gaacttctta tttgccaatc tctacaatca
    22261 ccctgccttc tttcaattca gggagacgca cggccttcaa ggccggatct gtttctgtgt
    22321 aaactttacc gtcaagatgg acgcttttga tgatcaaatc cagatcttcg ataggcagcg
    22381 ggtacaacaa gtccccagtg cgggcggccg tgatcttaaa atgaagctgc accggcctcc
    22441 ggttcaccca aagggccgtg tagaggtaat tgagcaagct tgtttccata ttgtggtaag
    22501 aggccttaaa ccggttggct ttggttccgt cttttatccg gccatccaaa aaaacgccca
    22561 ggtaggtgtc cccgtattca cgatcgatat aaaatttatc ccagaaagcg gctcccaggt
    22621 cgaagaggct gagatattcc tcccttccgg tgcctttaaa tagacagagg tcaaacatca
    22681 ttccatattc ctggatccac cagtacgaaa aatcggcatg acctccggaa tggtcttttg
    22741 catgggaagc aaaccacact ccgctcccgg tatccagacc ggaccggtgg atggcttccg
    22801 caaggcttaa tgcctccttt tcaaaccggt cgtttggatg aagcaggtga aggcgaagaa
    22861 gcatccaggc cgtctcgata ttatggccgg tattgatctc ggtcacggtc ggcggcgggg
    22921 ctttataatt ccagtcccgg tcaaaggatt ccaggatcca gcctgaatct ttatcccgca
    22981 ttttgtccag cacggtattc atgattcgtt cagcctgacc gagataaacc tcgtcgcggg
    23041 tagccaggta tagataaagc aggtatccgg atacaggagt aatcaccgag ctgaaggatt
    23101 tgctgctgtc cactacggtc cagtcgggat tcaattcatc aaaatatccc cctgaaaccg
    23161 gatcccaggc cttggtttcc aaaagggaat tggcttcgcg gatgtacttc aaaacttccg
    23221 gatcgagcgt gatgaaataa tacatgacca ggccggtaat tgaataaacc tggacgaaaa
    23281 tatttttatt tgcccgggcg gcttccccgc tcaccgtcag catatcataa aacccgccgt
    23341 gggctttatc ccaggccaca tttaataaat aatccttcag ctgctttgcc gtctccaggt
    23401 gcgccggatc cccgctcata aggtaagcgg ccgaatatcc gaataagtgc ctggccacca
    23461 tgctgggata tcgcatcgaa tccccatagg gcttccattg ccggtcgagg gtggtgtgaa
    23521 atccgccatg ctcccggtct gcggcatact gagtccagta aaggatgatg tcttccagtc
    23581 cctgcttttt ccagtaccct tcctcccgga acgggaattc cggccgggca ggtttacagg
    23641 agtaaaaaga gagaatcaga aacaggacgg ccgtcagggc cgcataaata ccgattctat
    23701 ttttcatatt ggaccatttg gcgggaacag gtaccgtcac tgtaaaattc caggacggca
    23761 taggcaggcg aaaactcctg gaaagaacca ttccaccaat tgccgctcac ggcgccgtca
    23821 cagagatacc ggatgccgag ataggtcagg tcatcctgaa gatgaatgtg gccgctgatg
    23881 caggttttga tctggggatg ggaggcaaag agctttttga gccttaaaaa atcggtatgc
    23941 caaaggttgc gttggatttt gaggtctccg ttggattccg ttttattaaa aaacaggccg
    24001 gcgcaaatgg acagaatggg gatgtgggaa accaggcaga cgtattcctc tttgggtatg
    24061 ccgctcaatt cggactgcag ccattcaaat tgcggttcgt ccagttttcc gatataaccg
    24121 ccttcgggat tgagctgggt gctgtccagg atgataaaat gccagcggcc tttggtaaac
    24181 cggtaatgcc tgccgggcat gccaaaggtt tccaccaccc aggttttgcc gtacagccgg
    24241 tcctggtctt ccagtgcctt gttttttatg aaccatcccc aggtatcgtg attgccgatg
    24301 caatgataga caggcaggct gttttcgttt ttcaggatgg ttttaaacag gtcaaactgg
    24361 atctgtgttt tttgtttgtc ggcttcgagg gcgtccatga tggcatcccc tccgttaatg
    24421 ataaaatcga ccttgggttt gagttgctgg acatgctgga gcgctttggc caagccggtt
    24481 tcaggaacga tcccgggttt cacatggata tcgctcagat gggccaggcg caccaggggt
    24541 ttccggggtc gaggtgagga ttcgggaagg gctgggagga ggaggcccga agcgaggccg
    24601 atggaagagt tcctgataaa ttttctgcgt tgcattggat taggacttga attcagcaaa
    24661 agtagatgat ctgcccgagt ttcttcaccg ggtatttgca gggtccactt gaaccttatt
    24721 ggctaaggcc cgaaacagaa gccgggtatc gggaagggcg agggaagacc cgggtaaggg
    24781 tccgttcaca tgcccggtcg tgggggtttt gcaggggggg ccgtcggatg gttgcctttg
    24841 gtgttcaata tacggaaata ggcatttcct ccactgacaa taaaaagccg gttgaattct
    24901 ttccccccaa aacaaagatc ggtgaccggt ccgttgggtg cgggcagaat caggtttacc
    24961 cggccaagct ggtcggagac ctggaccccc atattggtac ccgtgtataa gaggttggcg
    25021 gtgtcggtgg ccaggccatg gggactggct gttttttctc cataaggaat atggtaatga
    25081 atgtattcct ggccgtggct gagacttccg tcgggattaa ttttatacga ataaccgaac
    25141 acctcatccg gataaatgag gttcaggtaa gatttttccg aggttatggc catccctgaa
    25201 accccagatt ttaaaggatg caggatcgtt tttttggttt cataatgata atacccaagc
    25261 tgaaggccat tcataaaata caatcctttt gaggtcgaaa ggacttgtcc gggtttagcg
    25321 gagcggatca gtgtcgaggc tttgttgttt tgttctatat agatcgcccc ggccccggca
    25381 tcgctgtaaa tgggatggcc tgaggcatca aacgtaagat cgttgatgtg gccctgaacg
    25441 attcttttga aaatagtagc cggatcgaaa gaaacggtat agatggacgt tccgtctgtg
    25501 ccggtgaaat agaccgtacc atccggcgcg gctgcgattt ggttcagccc atcctttgaa
    25561 acagggagtt ccgaccactc acccaggttc ttggtgaggg acattccccg gtaccggtct
    25621 atagggctgg tcacaggagc gggataatct ttccagagcc agcgaagcgc atcggcgatg
    25681 acggtgaggg tatgggtatt gttgtgccct ccttcccccc aggcatggtt tacttcatac
    25741 ccggcccagg tcagggcgct gagcaggttc tggttggcga tataccaatc tccggcataa
    25801 atgttcagat cgtggtatcc gtcttccagg aagatcctca gaggtttggg ttcggtttta
    25861 cggaccaggg tggcggtttc atccgctccg cgcaaaccta cataggtgcc gatcgtgctt
    25921 aagaccctcc ggaatgcatc cggcctttcc caggccgcat tgaacgcgca aatagcgccg
    25981 ctgcttgctc ccgcaatgct ccggtcattg ggatcatcgc ttagattata ggattttttt
    26041 acctcgggaa ttatttcttc cattaaaaac cgggcataac ggtcacccag tccgtcgtat
    26101 tcaaagctcc ggttgaaacg ggggtaatgg gacgtgtcgg ctgaaatcac ccggccgtgg
    26161 tcaatataga tgccgatgat gaccgggatt tccccacgat gaatcagggt atccaggtgg
    26221 gcggacaggc gccagccatt ggccctcccc agtccgtcct gcacgatcat gctgcaggcc
    26281 gctttgcggg aatcatattg ggccggaata tagagccagt aattccgctc ggttcccggg
    26341 taaattttac tgatccattt ataagggcct ttgatttcac cggtgggaac ctgggccttg
    26401 gcaccaaggg ccaaaaggag gaaaattatt ccaatagaaa ttctgggcat cctgtctttt
    26461 attgaacaga atgaagatat gggaaataaa tannnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnaatcagat tataaagatt ggttggtttc cccgacgaaa cagggttttg
    26641 ggattaaacg gcgaaggccg ggattaaaac caggaaaaat aagtagcaat gaatacaatg
    26701 gccaggacaa aaacaagggc gatcaataca cagaatacga tccccaggat ggccatgagc
    26761 ggatttttgt ccttttttaa tcctttcaaa ccgaaaataa aaccgccgac agccaaaggt
    26821 aaagataaca aagacagggg gcttgcaaac aggaatagaa atccggcgcc tcccaggatc
    26881 agggaccaca tgccattccg gtttggtttt tcggccttaa ggccggtttg gaataaagca
    26941 tcgtttccct gtatcctacc ctgggccact tttttattta agcgatgctg ggcgtaacga
    27001 aacagcttcc ttttgatcca ggcctgttca tgaccgtttt tcctgctgat cagaccggga
    27061 tccagggaca aaaactggtt aaccgtcaaa gtcgcggtct tcgtttctgt taacgggctg
    27121 gccgggtgta cggcaaaagc agccatactg aacaagagaa atatggggag gcaaaacaat
    27181 cgaataaaaa tattcatggt ctctttctgg caaggttgta aacgacggac aagataaacg
    27241 gaatgcacga atcaataccc tgtggaacaa gggattcctt tcagaaacaa aatacccctt
    27301 tcctttttta ttgatccttt ttcttaattt cctttttata gtttttaaac atcttacccc
    27361 attgcctttc tttcctgcgc cgttccgcca gcgtggattc ataatgggct ttttcccttt
    27421 ccattttgag gtagttttca taagaggcct ggtctatatc gccttgctca accgctttga
    27481 tcaccgcgca tcccgtctcc ccggtatggc tgcaatcttt gaatttgcaa tcccgggcaa
    27541 gggaaaggat atggtcaaaa gtggtctcca ggccggagct gctgtccgca acgcccacct
    27601 cccgcattcc cgggttgtca atcagaatag ccccgttgtc gagaaggatc agttcgcggt
    27661 ggctggtgac gtgttttccc cgctggctgc tttcactgat ggccccggtc ttcatgacgg
    27721 tccgtccgga caggttgttc aggagggtgg atttgcccac gccggaagaa cccagcaggc
    27781 aataggtttt cccttttacc atcaaacttt ccaatgcggc atatccttcg cgggtttcat
    27841 tgctgatggc gatcaccggt acgtccctca cccttgtttt gatctcctgc aatatttcac
    27901 cggtccggga tggttgcgca aggtcggtct tggtcaggac gatcattggc tttactcccg
    27961 attcatggca gagggtcagg taacgctcca gccggtttat attaaaatca cggtccacgg
    28021 cctgcaccag gaaagccccg tcgatattgg tggcgatgat ttggctctcc ccgctgcggc
    28081 cgacggcctg ccttttgatc acggaagttc ggggcagaat ggcatggatg agggcaaaat
    28141 cgggatcgtg gatgctcagg gccacccagt ctcccacggc cggaaaatcc tctcggccgg
    28201 tcgcggaaaa acgaaggtgg ccggtgattt cagcctcgta ttccccacgg gcggtggcca
    28261 ccaggtaacg ttccttatgt tcttcaataa tcctcccggc ttcaaattga tcgagttggt
    28321 tttcgacccg ataaggatcg agggagggtg gatagcctaa ttgttcgagg gtcatgggta
    28381 cggaaaggta taaaaataag ggggaatgat gatatgcagt gtttcatgcc cgagccttag
    28441 gaaatattaa gaattaaaga aatgaagaat taataaagtt ctcaaaatct ttcaatcaac
    28501 ggaatacccg gcttgagata aatccatggt tctctttata tccccacgct ggattcacag
    28561 aaaggagtgg aaatagaata ttaaaaaccc aaagcctatg gattctgttt tttaacccaa
    28621 atcatgcaat agagtttcgt aatgaaaacc ggaaggccaa taaag
//
LOCUS       DAOQXT010000059        28314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1947_length_28314_cov_6.663152, whole genome shotgun sequence.
ACCESSION   DAOQXT010000059 DAOQXT010000000
VERSION     DAOQXT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 28314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28314
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1947_length_28314_cov_6.663152"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKM27_11225"
     CDS             complement(<1..920)
                     /locus_tag="PKM27_11225"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR07877.1"
                     /translation="MKKAFWITLALTILVSFRPMTDPNRYFEITKNIELFANLYKELN
                     YYYVDDIEPGRLMRTGIDAMLNSLDPYTNYFSEAQVESYRFITEGKYHGIGAGMATMD
                     GLPTITELFEESPAYKSGLRLADQILSIGGKAVKGKSKEEIDTYLQGSADTNIDLVVK
                     RPGEAKELQFSVTRGEVDVPNVPYSGMVSDKIGYINLTVFSDQAGSHVAGALQNLRKE
                     NPGLSGLILDLRDNGGGLLREAVNICNIFIPKNELVVTTRGKVKEWDRAFKTLQNPVD
                     ETLPLVILVDKGTASASEIVSGVIQDDFRSG"
     gene            complement(1166..1573)
                     /locus_tag="PKM27_11230"
     CDS             complement(1166..1573)
                     /locus_tag="PKM27_11230"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNR07878.1"
                     /translation="MIDLSKILAVSGMPGLYEMVANRADGAIVKSYETGQARFAPLRK
                     HQFSLLETISIYTTEDSTPLSEIFQHMKNSDLPVPDASADQKSTIDYFQKILPDYDPD
                     RVSIGDIKKVIKWYRFLNSKNLLKEKAEKEAGA"
     gene            1710..2684
                     /locus_tag="PKM27_11235"
     CDS             1710..2684
                     /locus_tag="PKM27_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HNR07879.1"
                     /translation="MLYDLIIIGGGPIGLVCGIEAKKTGLSHLILEKGTLVNSIFHFP
                     ANMTFFSTSILLEIGNVPFISHADKPTRTEALEYYRRIADAWNLNIHLYEPVREILKQ
                     EAAFRIRTDQKTYLAKRVILATGFYDQEVRLGIPGEELPKVRHYYDEAHPYFRQKVAV
                     IGAGNSACDVALECWQKGAEVTLIVRGPQLKESIKYWIKPNIENRIRAKDILTYFNAE
                     VMEIRPENLLVRQEDKVFEIENDFVLAMTGYRPDYAFLESAGVAIEGDEFRTPVHDPV
                     TLETNVPGLYLAGVIIGGLKTNKWFIENTRDHGKKIMDHILQKENNDV"
     gene            2677..3327
                     /locus_tag="PKM27_11240"
     CDS             2677..3327
                     /locus_tag="PKM27_11240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR07880.1"
                     /translation="MFKSIELIIFDCDGVLVDSEPLANSLFARLCREHGFHLSDEEAM
                     EQFPGSRFASCVAYVEQKHGRQMPGDFVLTYRQALSEAFASHLEPIPGVREILPSIQR
                     PKAVASNGPKEKMVDNLITCRIYHHFQQDHIFSAYDIQKWKPEPDLFLTASAHLGAAP
                     ATCLVVEDSQPGVQAALAAGMQVVGFTHGGRNKKLYNLGVPLIDQMTELKGILVKG"
     gene            complement(3343..3918)
                     /locus_tag="PKM27_11245"
     CDS             complement(3343..3918)
                     /locus_tag="PKM27_11245"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNR07881.1"
                     /translation="MPGLAGENIYIGMVVADPFSERMAVIEINGILERFGKMGEKSGW
                     TYVLIPAEIARSLSKARNTFRVKGHIDQHEIRQVALVPVGDGDFILPVNGGMRKAIKK
                     SAGAPVRLRIQHDDSELLPDRDFMECLAEEPLAMTHYQKLTQGHKNYFTKWIGSARTA
                     PTKASRIAMALDALSRGLGFAEMLREKKGIQ"
     gene            4265..6571
                     /locus_tag="PKM27_11250"
     CDS             4265..6571
                     /locus_tag="PKM27_11250"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNR07882.1"
                     /translation="MKIQTAEESLAKGDYYSALDWFEQAYKEKRDPEIAYKIALLHLT
                     LRDYARAETQLSRLLTTNLRGVRPPAEAYFYYGQVLKMNGKTREATEAFQKYMAEGKD
                     QNKISMAQVELDGIKMMDDLNPNKGIAVVNVGTLVNSPFTESSALQAEDGSLYFISFQ
                     KKAPIVQDGKDNDYFSKIYTSTKDKDGKWAKPTALPDAINRPNTHSGHVSLSPDGKSM
                     VFTRAVLVNNEVSETRLYASSKTASGWTPAQEVKTPTGDFQIRYPAFGRMFDREVLFF
                     SSNMPGTKGGYDIFYAHFDGETLGDIINMGPAFNSEGDEISPFYTRTKFYYSSNGLPG
                     MGGFDIFSSEWTGTEFAAPVNMGRNFNTSVDDMFFTLNPDGNTGFLVSNRPGTRSLKS
                     KTCCDDIFYFSLQPILVDLTVTVFETPKKALPGSQVILNEFVEESSIEKERKSDKGNT
                     YPFELEADKAYRFITSKEGYYPDTFDINTVGVMESSSLKREVILRPMPPEPTTEVITI
                     NEPIRLNNIYYDYDDAAILKDAEQDLSALLGLLQEYPTMVIELSSHTDARGNDEYNKR
                     LSQRRANSAKNWITARGIDASRIKAIGFGEAKILNHCKNGVNCTDDEHRFNRRTEFRI
                     LEGPQTITIKKEIFKGQKPADKQGGGEASSSIRNMKAVYPKGTPVLKWDNPFHDFGVV
                     QKGMEAVHEFGFTNTGDADLIIEMATAYECTRLEYPTRPVKPGERGAVKAIFYSPDKS
                     GETEITINIVANTNPVIAESRFRAFVKE"
     gene            6690..7310
                     /gene="pnuC"
                     /locus_tag="PKM27_11255"
     CDS             6690..7310
                     /gene="pnuC"
                     /locus_tag="PKM27_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNR07883.1"
                     /translation="MSDLLRTLGAQAVSLGWVQWASFLTGLAYIIQAARNNPSCWPWG
                     ILSSGLWAWASWFQLHLLSDALLQVFYVVLGFVGWYHWAAAKGTQNSKVLAVSSLKSK
                     ELVYWILASAGLSLILGLVMRPTTAAFPFTDAALTVYSVSATLMLVKRKMENWIFWIV
                     IDLVSIPVFIMRGGYLFALLFFIYTLIAWRGWKNWQSAIARAGKTA"
     gene            7384..8454
                     /locus_tag="PKM27_11260"
     CDS             7384..8454
                     /locus_tag="PKM27_11260"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HNR07884.1"
                     /translation="MKFQFSCILFLFFLSACKNDFMGESKTPQVDAFGEANHVVIVCD
                     PELWEGPLGDSITWYFGSAYPILPQPEPIFDLRPMSYSDLLGAKPRRELRNYIFIADL
                     SRVDSTSLRLLHSLVGEERLKKHLEESRVSNLINRDVWARGQQLLYIIGKDPDDLYSR
                     IRTSFPALSKQIREFDRTQIKATAYAAGLNKGLAAQLANRYQIDLDIPADWLVATENE
                     RGTWLRKETEKSSSNIVMGKVPYLNASQLSTEGLKRIRDTFSRTFVRTDSPGDYMEIN
                     DQDLPLFIYQKNIDGNYGVEARGVWETKIEFMGGPFQTYLIQTPAKDSLLYIDLFVYA
                     PELDKRNLIQQLEEIIQTVHFK"
     gene            complement(8460..9986)
                     /locus_tag="PKM27_11265"
     CDS             complement(8460..9986)
                     /locus_tag="PKM27_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNR07885.1"
                     /translation="MKPILPLLGLLFSVTLGFAQATSGKPNIVVIFSDDHALQAIGAY
                     GSPHIKTPNIDRIAKEGAIFNNALCTNSICAPSRAVLLTGKYNHLNGQIDNRTTTVMD
                     TRQASFPKYLQENGYTTSWIGKWHISNTPRFFDYWKVLPGQGDYYNPDFIRMDSSLAR
                     IPGYATDIITDEALQWLDKGRAGDKPFCLVIGHKTSHREWMPDIRDLDAFKGKPFAVP
                     PTFFDKYEGRKAAAHQEMEIRDLRWDWDLKVNAGKLPIVTRMDAIQRTGWDAYYEKEN
                     ALLDTATMTREQIALWKYQRYMHDYMACVLSMDRNIGRVLDYLDRHGLAENTIVLYSS
                     DQGFYLGEHGWYDKRFMYEPSLHMPLVMRYPALIKPGTRVDKMVVSIDFTPTLLDLAG
                     VGIPPDMQGVSFRPLISGGKSASWRKSMYYHYYEYPDHHRVLPHYGVRTDRYKLICFY
                     EGSAKSWELFDLKKDPDELNNLIGQKRHQKRIGGLKNELRSLMVRYKDDQALSHLGLD
                     "
     gene            complement(10078..11172)
                     /gene="dprA"
                     /locus_tag="PKM27_11270"
     CDS             complement(10078..11172)
                     /gene="dprA"
                     /locus_tag="PKM27_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNR07886.1"
                     /translation="MKHPVEKVALSLLEGIGPVTARQLVAYTGEAVNVFHARRKELMS
                     IPGIGEKTVHAILNHKTAFEHAEAELAFAERQGIRILFYADKGYPYRLKACEDSPVVL
                     YYKGQADLDHHRIVSLIGTRSPTEWGKGLCTRLVEELQEYQVLVVSGLAYGVDITAHR
                     RCLDLDIPTIGVLGNGLDKIYPSAHLPVAKKMTEKGGLLTQFPTRTKPDRENFPMRNK
                     VVAGMSDAIVVVESGKEGGSMITAEYANLYNKDVFAFPGRPTDPMSAGCNALIKKNKA
                     VLVESAADLVSLMNWDKTGIPAVQRSLFVELNEQEQKIVELLKKEESVCIDRLYLELN
                     QSPGAVAGILLELECKGVIKGLPGKRYMLI"
     gene            complement(11257..12828)
                     /locus_tag="PKM27_11275"
     CDS             complement(11257..12828)
                     /locus_tag="PKM27_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNR07887.1"
                     /translation="MKRLLLLTVLFCLTGTAISQDKADDKINAIIRKQGLEQSKVMDH
                     AFFLTDVYGPRLTGSDMTDRAVAWAVEELKKMGMQNVHTESWGPFGRGWELKHFEMHN
                     LEPTYYPIIGYPKAWTGSTNGMVTGEVIYVDANTDEELAKYKGKLKGKIVLLDTLREV
                     KEWFEPSATRREAENLLDMANADLPNQMGRGNFPRRFQTGAAFAGKIWDLINEEQPLA
                     ILDRSFKGDLGTVFVSGARAKQGMRVQDPGAVVIPQVTISVEQYNRMFRQIKAGLTVK
                     VSLELRTAYTNPEGMEYNIIAEIPGTDLKDEVVMFGAHFDSWHSATGATDNAAGSSVM
                     IEAARILLETIKESGIPPRRTLRLALWTGEEQGLLGSRAYVNKHLVTRDTFNNIREIK
                     PEQGKISAYYNLDNGTGKIRGVYQQGNSEVGPVFRTWLNPFKDLGAATLTLQNTGGTD
                     HLSFDAVGVPGFQFIQDEISYSTKTHHSNMDTWDHLLADDLKQAAMIIASFVYHTAQR
                     NEKLPRKTQPKIESN"
     gene            13011..14690
                     /locus_tag="PKM27_11280"
     CDS             13011..14690
                     /locus_tag="PKM27_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR07888.1"
                     /translation="MTTASLCGQINISFMVTDAACLDGTILIMATGGSGSYQYEIVGN
                     TCGLPNRSLQSSPEFRNLPPCTYTVLVLDGTGTSATRDVAVGGNYKEPSVTIKPDICG
                     FTIDVINGKPPLRFALSTDGGKTYGPPSAQNVYSGLMAGTYDIRVNDSCGYVIHKKVI
                     LPPDTLTYRFDRVQRNQVDSIVAVVTSGGQGPFRFYIVNGMDTLSSTYNTFALKDILL
                     SCSTKVVIETACGRSLQDFHWTDSATAGGPKDVIRVQRTDTICAGSRLVVGPSEYRMS
                     GEYLDTLKTMGGCDSLVLTRLTVDPPLAFDLEALHPLCSGQDNGSISVSGLTGYSPHS
                     FLLNGRSFQGPKAGQLTGGNYVVKITDGHGCSAEKNIRLIEPAKIEFEAGEDSLLQPG
                     ESIVLKVKTNLGKDEIKSIKWFADPDILCSHCETLTLSPTKTQKIRAELLSQEGCTAE
                     DAFTLRLHSGFRVFAPNILKIPSSDGSERNARFTLYGPQIETIEYLRIFDRYGSLVYE
                     IKESVPGDWTGGWDGYTGQKPAPPGAYIYVARVRFADESRQVLRGDVTVIR"
     gene            14780..16516
                     /locus_tag="PKM27_11285"
     CDS             14780..16516
                     /locus_tag="PKM27_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07889.1"
                     /translation="MQVVLFLFYLAGLFYPALQASESPPTKNLIFEFGLDPAGDAGVV
                     QGRLLLILSRDSTAEPRFQVNDGLRTQQLFGQDVNGWTKANRVRMDGKVLGYPVSSLN
                     DLPAGRYFAQAVVHLYETFHLSTGQEVKLPMDRGEGQQWNRAPGNLYNQPVKIEIKPS
                     GTQRFKITIDQKIPPIPQPEDTRYVKHIRIQSKLLTAFWGRPMFLGAHVLLPEGFDEH
                     PEARYPLAIFHGHFPHDFDGFRTSPPDSNLKPDYSERFKLAGYNIIQQQEAYDFYKIW
                     TGPGFPRVLAIEIQHPTPYYDDSYAVNSASQGPWGDAITYELIPYIENQFRGIGKGWA
                     RFTYGGSTGGWEALAVQVFYPDEYNGCYAACPDPIDFRAYCLINLYEDKNAYYYEGPF
                     KKMLRPGVRNYLGQISTTVKDMNQRELVLGSHSRSGDQWDIWEATYSPQGRDGYPERI
                     FDKTTGVINPAVAAYWKENYDLSYILKRDWKKNGAKWKGRIHLYCGDMDNYYLNNAVY
                     LAESFLEATTDPYYDGEVDYGDRAEHCWNGDQTQPNAISRLRYHRYFIPKWTSEIQHR
                     APAGADLKSWRY"
     gene            16636..24039
                     /locus_tag="PKM27_11290"
     CDS             16636..24039
                     /locus_tag="PKM27_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR07890.1"
                     /translation="MGAFTVIYHLIFYLYLLTSSVWPSDNYPISQAAGMRIITITCPP
                     NAFSSCPEEEPQPFSSYSEFILAGGTVSSNCGIDTASFSLSREVVVFPSCPRSIQRTY
                     SVRDSCGGQDSCTYLVVINDNTTPVLVCPSTLVPCDNNILPPPFASYTAFLQGMLSIG
                     GSVTDNCSIDTASWRYIGESVEQNENCLTTVVRTYLIEDSCNNSALCNQKFIYIDITF
                     PSFTPARDTVLYLDANCFADTSVANLGGLSNVADNCGILEITKQDIVRPGCTDLDTLY
                     RIWTVRDSCHNAVSDTQFIRVFDTIKPLIDGPADTSYLCISEVPPPDTASLVLLSGCS
                     AGDYSKTVRDSVADSLCLNNKVLFRIFTFADGCGNTISHVQVITIRDTLPPVLDCPDS
                     IRVACDSEIPVPDPQTEVTAIDMCDGLIDAVFIRDSIENEACDHQKIIYRIYAAADLC
                     GNTSRCIQVITVRDTLPPVMTCPADTLVSCMAEVPQADTAMVSATDQCQGNLRIWHDK
                     DSTVNEICANKKTILRIYLAADACGNTSRCIQTILVNDQTPPEIICPGDTTVPCLSEV
                     PPVNTGMVTATDDCGQSPDILHIQDSIADPYCPNHKTIFRIYQATDACGNSSRCVQII
                     TIFDNEPPVIIGQSNTINLSCDETFTIQIPEAFDLCQGIVPVQVDSLVTDSLCPGTYK
                     LVFSFTATDSCGNTGTRFDTVSFRDDTPPFIECPPDAVINCTELTVFTLDSFLYYGGQ
                     VSDECGIDSSSFMFIGEEFTEEGENLIFTRKFKISDLCGNTDSCEYRITTSRECFVDL
                     ALKKTIPSSSQPFFAQAGGLVPFCVTVYNQGFVAADSIRVIDYLPDPGSKIITPGWID
                     HQNGNFCIYLSQANQRLPKGGLKPGDSLTFCFEIRLSPGLRVSELVNICEVSETRDTS
                     MALLQDLDSHNDEIKSNDTGGEPGTANDDFIMGDSRMGEDEDDHDPSLFYICQPLNCI
                     NKTNASVQADPGCGHCFKASELLTGVLLPDEFYVVTLYDSKGKPLPSNCVGREYLGQT
                     LTYTVSLPACGQNSCWGKVTIEDKAPPTLDCSPDTVYCSQVSALPLPGPVPDNCSGMA
                     SVTLVTESWKDLGCAGGEIQGIYTRRLTSRDVWNNSITCDKNYYIRRINLQDIVCPRD
                     TLFECSDTADVTDPLVSGTPTIDGIALWPGGVTCKLFVTYRDQRKEFCGPGFKIVRTW
                     IIGDHCTGQEIKCIQTIAFEDRTPPLVTALNRNVRLNADPHECSAFLDIPKPEVIDCS
                     PVTYSYTITYFDPLDSIKARTVTGPLPARIRIPAGTEQKVYLHMVDECNWHTRDSITV
                     QVRDITPPNPVCKAVTQVTLDPDSCWGSIEAKNLDNGSHDNCCQDLHFAVAYMDEIEA
                     ARKSFLDSLQRACGKNEYLKYKGWYDAYLEDWINSYVFRDKLDLNACGSRQVVLRVYE
                     ACGLPKYDPHVFPCSPHAWYCYNTSWRYRVTFNQAFRNGAGTSKDCTVKAPWGCKSDL
                     VNKFSSMNAFQANFYESVWLPRTCDTLLGNFSAPVVCQPRLYNDCMVQVLADDKQKPV
                     CDSLPDLEVYCDGVPGGWAYASAFCPGSGDKYTTYPGAITRNGQGTVYGYYGGSYSAS
                     HDEHQVLDTACRSNHYKDGWQPVYCRAWLELDRFDTLYRPDPKQLFYQPVWTDKAHPY
                     RSLAAKEFRITDNCLIDTVFFEDRGNINGCGEGWLERVWTIRDRCGHTSTCSQKIRVK
                     HRSDFEVLFPEDREIGCTDLALADPDLAGRPQISDQDCEQIAVRFQDDTFESVEEACF
                     KIVRTWTVIDGCRYEPSDHHQDSEVIVDDRLRANENDRFCVFRNLKDNGDGIVQYIQV
                     IKIVDHTPPQVVCIDTTLCLSGPDCALLVDIPLEATDDCVTDLWFDLAFDHNQDGIFD
                     DEIRKGVRSITGSFLPGEYSFKITAYDHCGNKSECNNRLVIRDCKPPTPYCLNGVITV
                     IMPSTGAIEVWASDLDRGSYDNCTAPQQLKFSFDQAGTNLSRVFSCTDIPDGKQSTLP
                     VEIWVTDLAGNTESCKTFILLQDNGDSPGGACRDSSSLPGLVSVKGKLATEEKEPVEN
                     VEIRMLTGTNTMTRQSTGPDGFYHFPDVQTQGTMEISAHRDDNPMNGVSTLDLLLIEK
                     HIKGERPLSSPFKKIAADVDKNKEINVLDLVELRKLILGIYDRLPSGESWRFIPANYS
                     FKDPGQPFDYPSSVQLKGDQDNRLDFTGIKVGDINGTASPHSLTGTEIRQAGPGLIFT
                     IPDRQVRQGEWIEVPLRSPNFSGISGFQGTLHFEGLDYQGLIAASLPITIENTGYRWL
                     DQKDLTFSWHHGRSMEITEGQTLFLLRFRALNDLKLSESIWINSRHTVAESYEGRGEV
                     KNLSLQFQTASGRLALAEQRLFQNYPNPFSDATVIGLSLRENGKGKLNLLDVTGKLIK
                     TIEKDWEAGYQEIRLEQRDFPGPGIYFYQFESPFFNASRKMILRN"
     gene            complement(24101..24535)
                     /locus_tag="PKM27_11295"
     CDS             complement(24101..24535)
                     /locus_tag="PKM27_11295"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNR07891.1"
                     /translation="MQPDPILNQTLVQGKSVIDAWNSRDKSLIFSLYSTDFVREDLGT
                     NRQYDVAAVSQMLDLYWTAFPDLQFSLKDEISGKQGKTVLVWKAKGTHQGPFMNIPAT
                     NRPIEFYGISILHVKDGKIIKVLYSWDEASMLRQMGLLPNLR"
     gene            24648..25202
                     /locus_tag="PKM27_11300"
     CDS             24648..25202
                     /locus_tag="PKM27_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR07892.1"
                     /translation="MMAQFPENQVHTSRLRLVPFTENWVAAIQEGSQAFLALTGYRLP
                     QPYTEFPESFQALRTALDKEKPVFPWTSYALLWEPEKTYVGQGGFKGKPDVDGGVEIG
                     YEIAKGFRSMGFAHEAIGALVRLSFEQEKVRQVYAHTLPVLNASNHLLIKNKFVFDST
                     VTDEEDGQVWKWVLRKTVFLLSVE"
     gene            complement(25224..27308)
                     /locus_tag="PKM27_11305"
     CDS             complement(25224..27308)
                     /locus_tag="PKM27_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07893.1"
                     /translation="MCAKLSEILEHERKSAFVGRDAELDLFQSVLKKENPVLLLYFHG
                     PGGQGKTTLMKKCMDLCAREDVPYLHLDGREINAHPAGFTEALHARLQLKPDEDVFEA
                     LEKLQESHVLFIDTFERLHPFDDWIRQDFLPQLPAHFCTLILSRNPPGTSWAIDPGWK
                     KYMKAVEVRNLSPSQARQYLIKRNIPEKTIPAVLEFTHGHPLALSVAADLFDQHPGQD
                     FNPERSPDLIKTLLELFVQNVPGPAHRAALEISSLVHVTTESLLQEALGMEHIQGIFE
                     WLRNLSFFDKNESGIFPHDIAREALSRDVKWRNPDWYHSLHEKIRNYYIRKLNSTSHQ
                     AQRSVLFSLIYLHRLNPMVKPFFDWQENGTFWQDQLKPGDLPVLRALVGKWEGPEAEL
                     YFNYWINHAASETWVYRNSKSEAVAFCLKIEILKLKEQNELPDPAIKKVAEYVLARMN
                     PRPGDLFTAFRFWMADGTYQGVSGLQSVIFLSIVQYYFTPGLAVSFLSCAQPEYWKTV
                     LQYADLSRVPELDFVSDGIPFGWYVHDWRKRPPLAWLDLLGKREVELVDQYDEASTEA
                     VSMLVLSEEEFGDSVHQAVKYYHQPDQLLSSPLLRSRLVLRMIMPDMKDLDKLKILKA
                     RIDLALKEIEDSPIDGKYHRVLYRTFINPVGSQEKVADFLNMSFSTYRRYLKTALEKL
                     VKLLWNMEMEKN"
     gene            27408..>28314
                     /locus_tag="PKM27_11310"
     CDS             27408..>28314
                     /locus_tag="PKM27_11310"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HNR07894.1"
                     /translation="MTQKISSLHNRLRQFADHLIEEEMKVYHELGIDIPHRWHAILQI
                     FNEDDQPHTITDLAELQNKTHPDVVYTVNQMMKHGLVREMTDRNDKRKRFIKASEKAK
                     QLMSELRPAWQAVQDATNSWVKEIAPDLWLNIEALNNSLEKKSFFQRIKKEKKRADLR
                     NVLLSRFEEVQNGKMLLQNFWNQFKGKYFNIDALDEFILNIDEHITKNQAQVYFARWG
                     EPIIGCICLIRRSFKFCEIVHLWVDENYRRRYVGTSLLNKAIAAGKEMGVNALFSQSH
                     PKLVAANSLFQHAGFSVSDQFPKAAE"
BASE COUNT         6842 a         7495 c         7106 g         6871 t
ORIGIN      
        1 ccgcttcgga aatcgtcctg gatcaccccc gagacgattt ccgaagcgga agcggtgccc
       61 ttgtccacca ggatcaccag gggcagtgtt tcatccaccg ggttttgcag tgttttgaat
      121 gcccggtccc attcctttac cttgccgcgg gtcgtcacca ccagttcatt tttgggtata
      181 aaaatattgc agatgttgac ggcttcccgg agcaagcccc ccccgttgtc cctcagatcg
      241 aggatcaggc cgctgagtcc tggattttcc ttacgcagat tttgcagagc ccctgcaaca
      301 tggctgccgg cctggtcgga aaatacggtc aggttgatat agccgatttt atcgctgacc
      361 atgccgctgt aaggcacatt gggaacatcc acttccccac gggtaaccga aaattgcaat
      421 tcctttgcct ctcccggcct tttgaccacc aggtcgatat tggtatccgc agaaccctgg
      481 aggtaggtgt cgatttcttc tttggattta cccttaacgg ctttgccccc aatagacagg
      541 atctgatcag ccaggcgtaa accggactta taagccgggg attcctcaaa aagttcagtg
      601 atggtcggaa ggccatccat cgtcgccatg ccggccccga tgccgtggta ttttccttcg
      661 gtgataaaac ggtaactctc cacctgggct tctgaaaaat aattggtata gggatccagg
      721 ctgttgagca tggcgtcgat ccccgtacgc attaagcgtc ccggttcaat atcgtccacg
      781 taataataat tgagttcttt gtacaggttg gcaaacagtt ctatattttt tgtgatctca
      841 aaataccggt tgggatcagt catgggccgg aaagagacca ggatggtcag ggcgagagtt
      901 atccagaacg cttttttcat tggaaaaatc gattagccgg taaaattact ggttaatccc
      961 gcacccaggt tcttaacaat taattaaaac tttatgccgg aaactaccca agacttcggc
     1021 ctgaggcttg atgcggatgc gaaaaaatta aggggctaat aacccctttg cctgaataag
     1081 gttttgatca gcgtacaaat tcccgccttg aaatcgcgtt ggctaattat atgccggtat
     1141 tgggcagccg gccctggcct gggtatcagg cacccgcctc tttctccgcc ttttccttaa
     1201 gcaggttttt actgttcaga aaacggtacc acttgatcac ttttttgata tccccgatgc
     1261 tcacgcggtc cggatcgtag tcgggtaaaa ttttctgaaa ataatcgatc gtgctctttt
     1321 ggtcggctga agcatccggc accggcaggt ccgaattttt catatgctga aagatttccg
     1381 acaggggggt ggagtcttcc gtcgtataaa tgctgatggt ctctaataat gaaaactggt
     1441 gtttgcgcag aggagcaaat ctggcttgcc cggtttcata ggactttacg attgccccat
     1501 cggcgcggtt ggctaccatc tcataaagac cgggcatccc cgataccgcc aatattttac
     1561 tcaggtcgat cattttgcaa aaataagcac tcaacagtca gttttcatca gtcagggctg
     1621 gtctctccaa ttttgcttcc tttacctttc gcctttcacc tttctccttt cgcccttcct
     1681 cccttcctcc atatctttgc cccccggata tgctgtatga cctgatcatc atcggaggag
     1741 gccctatcgg gcttgtgtgt ggaattgaag cgaaaaaaac gggtctcagc cacctcatcc
     1801 tggaaaaagg cacactggtc aactccatct ttcacttccc ggccaatatg acttttttct
     1861 ccacgtccat cctgttggaa atcgggaatg taccttttat ctcccatgcg gataagccta
     1921 cccgaaccga agccctggaa tattaccgaa ggattgccga tgcctggaac ctgaacatac
     1981 acttgtatga gccggtccgt gaaatcctga aacaggaagc ggccttccgg atcaggaccg
     2041 atcaaaaaac atacctcgca aaaagggtga ttcttgcaac cgggttttac gaccaggagg
     2101 tccgtctggg cattccgggg gaggaattgc cgaaagtccg gcactattat gatgaagccc
     2161 atccctattt ccgtcagaaa gtagccgtca tcggagcggg caattccgcc tgcgacgttg
     2221 ccctggaatg ctggcagaaa ggagctgaag tcaccctgat cgtccgtggg ccacagttga
     2281 aggaaagcat caaatactgg ataaaaccca atattgaaaa ccgcatcaga gcaaaagaca
     2341 tccttactta tttcaacgcg gaggtgatgg aaatcaggcc tgaaaacctc ctggtgaggc
     2401 aggaggacaa ggtatttgaa atcgaaaatg attttgtgct ggctatgacg ggataccggc
     2461 cggattatgc cttcctggaa tccgcgggcg tcgccattga aggggatgaa ttccgcactc
     2521 ccgtgcacga tcccgtgacc ctggaaacca atgtccccgg actctacctg gccggcgtaa
     2581 tcatcggagg tctaaaaacc aacaaatggt ttatcgaaaa cacccgcgac catgggaaaa
     2641 aaatcatgga ccatattctt caaaaagaaa ataacgatgt ttaaatcgat tgaattgatc
     2701 attttcgatt gcgacggggt cctcgtcgac agtgagcccc tggccaattc gctttttgcc
     2761 cggctttgca gggaacacgg ctttcacttg tccgacgagg aggccatgga acagtttccg
     2821 ggcagccgtt ttgcgagctg cgtcgcttat gtggaacaaa aacatggccg gcaaatgccc
     2881 ggtgattttg tattgactta tcgccaggcc ctgagcgaag ctttcgccag ccatcttgag
     2941 cccatccccg gagtgaggga aatcctgcca tccatccaaa gacccaaggc ggtagccagc
     3001 aacggaccca aggagaaaat ggtggataac ctgattacct gccggatcta tcatcacttc
     3061 cagcaagacc acatattcag cgcttacgat atacaaaaat ggaaaccgga accggacctg
     3121 tttcttaccg cctccgccca ccttggcgct gctcccgcaa cctgccttgt agtggaagac
     3181 agccagcccg gagtacaagc cgccctggcc gcaggcatgc aggtcgtggg attcacccac
     3241 ggagggagga acaaaaaatt gtataacctg ggtgtgcccc tgattgatca gatgaccgaa
     3301 ttgaagggga ttttagtgaa ggggtgactc ggagtcaact cttcactgaa tccctttttt
     3361 ttccctcagc atttccgcga accccaggcc ccgcgacaaa gcatccaggg ccatggctat
     3421 tctcgaagcc ttggtcggtg ccgtccgggc cgaaccgatc catttggtga agtaattttt
     3481 atgcccctgg gtcagttttt gataatgggt catggccagg ggctcctccg cgaggcattc
     3541 cataaaatct cggtccggaa ggagttcact gtcgtcatgc tgtatcctca accggaccgg
     3601 ggccccggcc gattttttaa tagcctttcg cattcccccg ttgacgggca ggataaaatc
     3661 cccatccccc acgggaacca gggccacttg cctgatttca tgctggtcga tgtgtccttt
     3721 aacccggaag gtattgcggg ctttggacaa ggaccttgca atttccgccg ggatcagcac
     3781 gtaagtccat ccggatttct cgcccatctt gccgaagcgt tccaggattc cgtttatctc
     3841 tatgactgcc atccgttcgg aaaagggatc tgcaaccacc atgccaatat aaatgttttc
     3901 tccagccagt cccggcacca ctcctgctct ctttttaatt taatgatgac cggggatatg
     3961 gcctgatttc ttttacccac ctccaaggca gtgacctggt ggtggtcatc gaaaggtcaa
     4021 gaggttttat tattgacctg ttacataaaa gcctgagccc cgaaatggcc cgtattttgt
     4081 ggagttaagg tattcaaaag ggtttgcagg ccttatcgcc tgaaaaagaa atcctttatc
     4141 tttatcttta aatcgtacat tctgatcaac acaggccaat atgtataaaa tcctgattgc
     4201 gcttatctta atggttgtgg ctccttccgc atatagtcaa ccgctgaagg aaataccggt
     4261 atccatgaaa atccagactg ccgaggaaag cctggccaaa ggagattatt acagcgccct
     4321 ggactggttt gaacaggcct acaaagaaaa acgggaccct gagatcgctt acaaaattgc
     4381 tttgttgcat ctcaccctga gggattatgc ccgcgccgag acccagcttt cacgcctgct
     4441 gaccaccaat ctgagggggg tgaggccgcc ggcagaagcc tatttttatt acggccaggt
     4501 attaaaaatg aacgggaaga cccgggaagc caccgaagct tttcaaaaat acatggccga
     4561 aggcaaggac cagaataaga tttccatggc ccaggtagaa ctggacggca taaaaatgat
     4621 ggatgacctg aatcccaaca agggcattgc ggtcgtcaat gtaggcaccc tcgtcaattc
     4681 tccgtttacc gaatccagtg cgttgcaagc cgaagacggc agtctttact tcatttcctt
     4741 ccaaaagaag gctcctattg tacaagacgg aaaagacaat gattattttt ccaaaatata
     4801 caccagcacc aaagacaagg acggcaaatg ggccaaaccc accgccctgc cggatgccat
     4861 caatcgtccg aatacgcaca gcgggcatgt ctcgctgagc ccggatggca aaagcatggt
     4921 tttcacccgg gccgtattgg tcaacaatga agtttccgaa acgagacttt atgcctcttc
     4981 gaaaacggcg agcggctgga cgccggcaca ggaggtcaaa acaccgaccg gagacttcca
     5041 gatcaggtat cccgccttcg gaaggatgtt tgaccgcgag gtgcttttct tttcctccaa
     5101 tatgcccggt accaaagggg gttacgatat tttttatgcc catttcgacg gggagaccct
     5161 gggtgatatc atcaatatgg gacctgcctt taattccgaa ggggatgaaa tcagcccgtt
     5221 ttatacccgc accaaattct actacagttc caacggcctg ccgggcatgg gtggattcga
     5281 tatcttttcc tcggagtgga ccggtacgga attcgcagcg cccgtaaata tgggacgcaa
     5341 tttcaatact tccgtggacg acatgttttt cacgctcaac cccgatggaa acacgggttt
     5401 tctggtttcc aaccggccgg gtacccgaag cctgaaaagc aaaacctgct gtgatgatat
     5461 tttttatttc agcctgcaac ccatcctcgt ggatctgaca gtgacggtgt ttgagacccc
     5521 caaaaaagcc ttacccggca gccaggtgat cctcaatgaa tttgtggaag aaagcagcat
     5581 tgaaaaagag cggaagagcg ataaaggaaa cacctaccct tttgaactgg aggcggacaa
     5641 ggcctaccgg ttcattactt ccaaagaggg ttattatccg gacacttttg atatcaatac
     5701 cgtgggcgtc atggaatcca gctcgctcaa acgggaagtc atcctgcgcc caatgcctcc
     5761 cgagcccacc accgaagtga tcaccatcaa tgagcccatc cggctcaata atatttatta
     5821 cgattacgat gacgccgcca tcttgaaaga tgccgaacag gacctcagcg ccctgctggg
     5881 tctccttcag gaatatccga ccatggtcat tgaactgtcc agccacacgg atgcaagggg
     5941 aaatgacgag tacaataaaa ggctctccca gcgcagggcc aactcagcca aaaactggat
     6001 taccgcaaga ggcattgatg cctccaggat caaagccatc ggtttcggag aagcgaagat
     6061 cctgaatcac tgtaagaacg gcgtaaactg cacggatgac gaacaccggt ttaaccgccg
     6121 gaccgaattc agaatcctgg aagggccgca gaccatcacc ataaaaaaag agatcttcaa
     6181 aggccagaaa cctgcggaca aacaaggcgg cggcgaagca tcatccagta tccggaatat
     6241 gaaggcggtt taccccaaag ggaccccggt gctgaaatgg gacaatccat tccatgactt
     6301 cggagtggtc caaaaaggga tggaagccgt ccacgaattt ggattcacca acaccggaga
     6361 tgcggacctg atcatagaaa tggctaccgc ttacgaatgc acccgactgg aatatccgac
     6421 ccggccggta aaaccaggag aaagaggggc ggtaaaagcc attttctata gtccggacaa
     6481 atccggggaa acggaaatca ccatcaatat cgtggccaac accaatccgg tgattgccga
     6541 atccaggttc agggctttcg taaaagaatg aataaaggac ccctctccct ggtgcccgga
     6601 ggtatggccc tttttatttt accggtagca tggaaaaagg aattgccctc ttcccgcatc
     6661 ttggaatcac ttgaataagg ggtatactca tgtctgatct cctcagaacc ctgggcgctc
     6721 aggccgtttc cctgggctgg gtccagtggg cttcttttct caccggactg gcttatatca
     6781 tccaggccgc acgcaacaat ccaagctgct ggccctgggg aatcctgtcc agcgggcttt
     6841 gggcctgggc cagttggttc caactgcatc tgctgagcga tgccctgctc caggtctttt
     6901 atgttgtttt gggttttgtg ggttggtacc actgggctgc tgccaagggt acacaaaatt
     6961 caaaagtctt ggcggtcagc agtttaaaat ccaaagaact ggtctattgg attttggcga
     7021 gtgccggcct ttccctcatc ctcggcctgg tcatgcggcc aaccactgcg gcctttccct
     7081 ttaccgatgc ggccctgacc gtatattccg tctccgcaac cctgatgctt gtcaaaagaa
     7141 aaatggagaa ctggattttc tggatcgtca tcgacctggt gtccatccct gtgtttatca
     7201 tgcgcggcgg ttacctgttt gccctcctct ttttcatcta caccctgatc gcctggaggg
     7261 gctggaaaaa ttggcaatct gcgattgccc gggcaggaaa aacagcatga aatgcccgcc
     7321 aatcctgccg gaaaatgcat ctataattgt gtattttaca ttcaaatacc atccttgatt
     7381 taaatgaaat ttcaattcag ttgtatttta ttcctcttct ttctcagtgc ctgcaaaaac
     7441 gattttatgg gggagtccaa gacccctcag gtagatgctt ttggcgaagc caatcacgtg
     7501 gtgatcgtct gtgacccgga gttgtgggaa ggacctctgg gggattccat aacctggtat
     7561 tttggttctg cttacccgat ccttccccag ccggaaccca tctttgacct gagaccgatg
     7621 tcttacagtg acctgcttgg ggccaaacct cgccgggagt tgaggaacta tatattcatt
     7681 gccgaccttt cacgggtgga ttccacttcc ctccggctcc ttcacagcct ggttggggaa
     7741 gaacggttga aaaaacacct ggaagaaagc cgggtatcca acctcatcaa ccgggatgtc
     7801 tgggcaaggg gccagcaatt gctgtatatc ataggcaagg atcccgacga cctttatagc
     7861 cggatcagga ccagtttccc ggccttgtca aaacaaatcc gtgaatttga ccgcacccag
     7921 atcaaagcga cggcctatgc cgccggcctc aataagggcc ttgccgctca actggccaac
     7981 cggtaccaga ttgacttgga tattccggcc gattggctgg tggccactga aaacgaacgc
     8041 ggcacctggt tgagaaaaga aacggaaaaa tccagttcaa atatcgtgat gggcaaggtt
     8101 ccctacctca acgccagcca gctttccaca gaaggcctca aacggatccg ggatactttt
     8161 tcccggacct tcgtccgtac cgattcaccc ggggactata tggaaatcaa tgaccaggac
     8221 ctccctttat ttatttatca gaaaaacata gacggcaact acggcgtcga ggcccgcgga
     8281 gtgtgggaaa ccaaaatcga atttatggga gggccctttc agacttacct gatacagaca
     8341 cccgccaaag actctctctt gtacatcgac ctgtttgtat atgccccgga attggataag
     8401 cgcaacctta ttcagcagct ggaagagatc atccagacgg tacacttcaa gtgacctcct
     8461 tagtccaggc ccagatggga cagggcctgg tcatccttat accggaccat caggctgcgc
     8521 agttcatttt tgagcccgcc gatcctcttt tggtgccttt tctgaccgat cagattattc
     8581 aattcatcgg gatctttttt aagatcaaac aattcccatg attttgccga accttcataa
     8641 aaacagatca gcttgtaccg gtcggtgcgg acgccataat gaggcaaaac ccggtgatgg
     8701 tccggatatt cgtaataatg atagtacatg gattttctcc aggaagcaga tttccccccg
     8761 ctgattaaag gcctgaacga tacaccctgc atatccggag gaatgcccac ccccgccaaa
     8821 tcaagaaggg tcggcgtgaa atcaatactc acgaccattt tatctacccg ggtaccgggt
     8881 tttatcaggg ccggatagcg catgaccagg ggcatatgca gggaaggttc atacataaac
     8941 cgcttgtcat accagccatg ctccccgaga taaaatccct ggtcggagga gtacaggacg
     9001 atggtgtttt cagccagtcc gtgccggtcc aggtaatcca ggacccgtcc aatattgcgg
     9061 tccatcgaca gcacgcaggc catatagtca tgcatatatc tttggtactt ccacaaggcg
     9121 atttgttccc tggtcatggt ggcggtatcg agcagggcgt tttctttttc ataataggca
     9181 tcccagccgg tgcgctggat ggcatccatc cgcgtgacga tgggcaattt ccctgcattt
     9241 accttaagat cccagtccca acgcaggtcc ctgatctcca tttcctggtg cgccgctgcc
     9301 tttcggcctt cgtatttatc aaaaaaggta ggaggtacgg caaagggttt gcccttaaac
     9361 gcgtccaggt cacggatatc cggcatccat tcccggtggg aggttttatg gccaatcaca
     9421 aggcaaaaag gtttgtcccc tgccctcccc ttgtccagcc attgcagcgc ttcatcggtt
     9481 atgatgtccg tggcataacc gggaatccgg gcgagtgaac tgtccatccg gataaaatcc
     9541 gggttataat agtcgccttg acccggcagc accttccagt aatcaaaaaa cctgggggta
     9601 ttgctgatat gccatttgcc gatccaggac gtggtatacc cgttttcctg cagatatttg
     9661 ggaaaggaag cctggcgggt atccattacg gtcgtggtcc tgttgtcgat ctgtccgttg
     9721 aggtgattgt attttccggt aagcaatacc gccctgcttg gtgcgcagat tgagttggtg
     9781 cacaaagcat tattgaaaat ggccccttcc tttgcaatac gatcgatgtt cggggttttg
     9841 atgtggggac ttccgtaggc gccgatggcc tggagggcat ggtcgtcgga gaagatcacg
     9901 acaatattgg gtttaccgga agtggcctgg gcaaatccca aagtaacaga aaagagcaga
     9961 ccgagaagcg ggaggattgg tttcatgaaa gaggctttgg atacaagtta gaaaaaaaaa
    10021 tcatgagccg gtatccggaa ccacttctgg cacaggccgg ttcccccatt accttcacta
    10081 aatcaacata tacctcttcc ccggtaagcc tttgatcact cctttgcatt cgagttccaa
    10141 caaaataccg gccacagcac cgggggactg gttcagttca aggtacaacc ggtcgatgca
    10201 taccgattcc tccttcttca acagttccac tattttctgc tcctgctcgt tcaactccac
    10261 aaagaggctt cgctggacgg ccggaattcc tgttttatcc caattcatta agctcaccag
    10321 gtccgccgcg ctttctacca ggaccgcttt attcttttta attaaagcat tacatcccgc
    10381 ggacatgggg tcggtgggtc tgccgggaaa agcaaagaca tccttgttgt acagattcgc
    10441 gtattccgcg gtgatcatgg accctccttc cttccccgac tccaccacga cgatggcatc
    10501 ggacatcccc gccaccactt tattcctcat cgggaaattt tccctgtccg gtttggtccg
    10561 ggtcgggaac tgggtcaaca aaccgccctt ctccgtcatt tttttcgcca ccggcaaatg
    10621 agcggatgga taaatcttat ccagtccatt ccccagcact ccaatggttg gaatgtccag
    10681 gtccaggcac ctgcggtgcg cggtgatatc cactccgtaa gccagacctg aaacaacaag
    10741 tacctgatat tcctgaagtt cttcaaccag gcgggtgcat aggcccttac cccattccgt
    10801 cggtgaccgg gtgcctatga gggaaacgat tctgtgatgg tccagatccg cctggccttt
    10861 gtaatacaat accaccggac tgtcttcaca ggccttcagg cggtaaggat aacctttgtc
    10921 cgcataaaaa aggatccgga tcccttgccg ctccgcaaaa gcgagttcgg cctcggcgtg
    10981 ctcaaaagcc gttttgtggt taaggatggc atggaccgtt ttttcaccga tcccggggat
    11041 gctcatcagt tctttcctgc gggcatggaa tacattgacg gcctccccgg tataggccac
    11101 aagctggcgg gccgttacgg gtccaatccc ctccagcagg ctgagggcca ctttttcaac
    11161 agggtgtttc attaaagtaa aattacaaac tcgccggacg atgggatggt ttccccggcg
    11221 gaaccgctat gtgcagtccg cactggggaa acccgttcag ttcgattcta ttttaggctg
    11281 cgttttccgg ggcagttttt cattacgttg tgcggtgtga tatacgaagg aggcgatgat
    11341 catcgccgcc tgtttgaggt catcggcgag caggtggtcc caagtatcca tattggaatg
    11401 atgggttttg gtgctgtagg atatttcatc ctggataaac tggaaacccg gaaccccgac
    11461 cgcgtcaaaa gacaagtgat ccgtaccccc ggtattctgt aaagtcaggg tggctgcgcc
    11521 caggtcttta aacggattga gccaggtcct gaaaaccggg cccacctcgc tgttgccctg
    11581 ctggtagacc cctctgattt ttccagttcc gttatccagg ttgtaataag cggatatttt
    11641 accctgttcc ggtttgatct cccggatgtt gttgaaggta tccctcgtca ccaggtgttt
    11701 gttcacatag gcacgtgaac cgagcaatcc ctgttcttcc ccggtccaca acgcaaggcg
    11761 cagcgtcctc cgtgggggaa tgccggattc tttaatcgtt tcaagcagga tgcgggcggc
    11821 ttcgatcatg actgacgaac ccgcggcatt gtccgtggcc ccggtggcgc tgtgccagga
    11881 atcgaaatgc gcaccaaaca tcaccacttc atctttcagg tcggtgcccg gtatttcggc
    11941 aatgatgttg tattccatgc cctcgggatt ggtataggct gttcttaatt caagggaaac
    12001 cttgaccgta aggccggctt tgatttgcct gaacatccgg ttgtattgtt ccacggaaat
    12061 ggtgacctgg ggaatcacga cggcgcccgg gtcttgtacc cgcatccctt gtttggcgcg
    12121 ggccccgctg acaaacacgg tgcccaggtc ccctttgaaa ctccggtcca atatcgcgag
    12181 cggttgttct tcattgatca ggtcccagat cttgccggca aaagcggccc cggtctgaaa
    12241 gcgcctcggg aaattgcccc tgcccatctg gttcggcagg tccgcattgg ccatgtccag
    12301 caaattttct gcttccctgc gggttgcgga aggttcaaac cactccttca cttcgcgcag
    12361 ggtatccagc aatactattt ttcccttcag ttttcccttg tattttgcaa gttcttcatc
    12421 cgtattggca tccacataaa tcacttcccc ggtgaccatt ccattggtgg agccggtcca
    12481 ggctttagga tatccgataa tgggataata agtgggttcc aggttgtgca tttcaaaatg
    12541 tttcagttcc cagcctctgc cgaatgggcc ccaggattcg gtgtgcacat tctgcatccc
    12601 catttttttc aattcctcga cggcccaggc caccgcccgg tctgtcatat cggaaccggt
    12661 gaggcggggg ccatacacat cggtgagaaa aaaagcatgg tccatgactt tactctgttc
    12721 caggccttgt ttgcggatga tggcattgat tttatcatct gccttatcct gagaaatggc
    12781 cgtccccgta aggcaaaaca ggacggtcaa taaaagcagt cttttcatta atatgaattg
    12841 gtttaaaata attaaagcaa tgcaagatag ccgaaattca tgtgaaatgc agccctgacc
    12901 acagcccccg ccaggccgtc ctttggcggc ccggggagat tggtgtaccg tagattttat
    12961 agtattttcg gcggatgggc cggttcaggc ttttttgcat actgaatttc ttgaccacgg
    13021 ccagcctctg tggccaaatc aatatcagct ttatggtcac ggatgccgca tgtctcgatg
    13081 gtaccatcct tatcatggcc accgggggca gcggctccta ccagtatgaa atcgtcggga
    13141 atacctgcgg actccccaac cggtcgctgc agtcgagccc ggaattccgg aacttacccc
    13201 cttgtacata cacggtcctg gtcttagatg gtacgggaac ctccgccacc cgggacgtag
    13261 cggtaggggg aaattataag gaaccttcag tcacgatcaa accggatatc tgtggtttta
    13321 ccatcgatgt catcaatgga aaacccccgc ttcgtttcgc tctttccacc gatggcggaa
    13381 agacctatgg tccgccttcc gcacagaatg tatactccgg tttgatggcc gggacttatg
    13441 atatcagggt gaatgacagt tgcggatatg tgattcacaa aaaagtgatc cttccgccgg
    13501 acacgctgac ctatcgtttc gaccgcgtgc aaaggaacca ggtcgattcc atagtagccg
    13561 tcgtcacttc aggcggccag gggccattcc ggttttatat tgtgaatggg atggacacgc
    13621 tgagcagtac ttacaatact tttgcgctta aagacatcct ccttagctgc tccacaaagg
    13681 tggtcattga aaccgcctgc ggccgttccc ttcaggactt ccattggacg gattccgcca
    13741 ctgcgggtgg gcctaaggat gtgatcaggg tacagcggac ggataccatt tgtgcaggtt
    13801 ccaggctggt cgtcggacca tcggaatacc gtatgtccgg ggaatacctg gacactttga
    13861 agaccatggg cggctgcgac agtctggtct tgacacgcct gaccgtcgat cctccgctgg
    13921 ctttcgatct tgaggcgcta cacccgttat gttccggcca ggacaacgga tccatttccg
    13981 tatccggact gaccggctac tccccgcatt cgttcctttt gaatggacga tccttccagg
    14041 gacccaaagc aggtcaattg accggcggaa attatgtggt aaagattacc gatggccatg
    14101 gttgttctgc ggaaaaaaac atcaggttga ttgaacccgc gaaaattgaa tttgaagcag
    14161 gggaggacag cctgttgcaa cccggtgaat ccattgtgct caaggtcaag accaaccttg
    14221 gaaaggacga gatcaaatcc ataaagtggt ttgcagaccc ggatatcctg tgctcccatt
    14281 gcgaaacgtt gacgctctcg ccgacaaaaa cacagaagat cagggcggaa cttctaagtc
    14341 aggaagggtg taccgctgag gatgcattta ccctccgtct ccattcgggt ttccgggttt
    14401 ttgcgcccaa tattttaaag attccatctt ctgatggaag cgaacggaat gcccggttta
    14461 ccctatacgg accccagatt gaaaccatcg agtacctgcg catattcgac cggtatggtt
    14521 cactggtcta tgaaatcaaa gagagcgtcc ccggggattg gacgggtggc tgggatggct
    14581 atacgggaca aaaaccggcg cctccgggag cgtacattta tgtcgcaagg gtccggtttg
    14641 cggatgaatc caggcaggtc ctgcgcgggg acgtcacggt gattcgttga gcttggtcga
    14701 cgggagaatc tttttatctt tatttaaatc aaaccctacc ggatatccaa tattatttta
    14761 ttccatttca ataaatgcca tgcaagtcgt cctgttcctt ttctacctgg ccggtctttt
    14821 ttatcctgca cttcaagcgt ctgaaagccc acccaccaaa aatctgattt ttgaatttgg
    14881 cctggatcca gctggggacg caggggtggt tcagggaagg ctcctgctca tcctgtccag
    14941 ggattcgacg gcagaaccgc ggtttcaggt caatgacggg ttaagaaccc agcagttgtt
    15001 tgggcaggat gtgaatggat ggacaaaggc aaaccgggtc cgcatggacg gcaaggtgct
    15061 gggatatccc gtttcttccc tcaatgatct tccagccggc cgctattttg cccaagcggt
    15121 ggttcatttg tatgaaactt ttcatctgag tacgggacag gaagtgaaat tgccaatgga
    15181 ccggggcgaa ggccaacaat ggaaccgggc acccggtaac ctctacaatc agcccgtcaa
    15241 aattgaaatc aaaccgtccg gaacccaacg gtttaaaatt acgattgacc aaaagatacc
    15301 tcccatccct cagccggaag ataccaggta tgtcaaacat atccggattc agtcaaaact
    15361 gctgaccgcc ttttggggaa ggcccatgtt ccttggcgcg catgtgctgc ttcccgaagg
    15421 atttgatgag catcctgagg ccaggtatcc cctggcgatc tttcacgggc atttccccca
    15481 tgattttgac ggattcagga ccagcccacc ggacagcaac ctgaaacccg attacagtga
    15541 acggtttaag ctcgccgggt acaatatcat ccagcaacag gaagcctatg atttttataa
    15601 gatttggacc ggccccggct ttccaagggt cctggctatt gaaatccagc acccgacccc
    15661 ttattatgac gacagttatg ccgtgaacag cgcgagccaa gggccctggg gcgatgccat
    15721 cacctatgag ttgattccct atatcgagaa tcaattcagg gggatcggca aaggctgggc
    15781 caggttcact tatggagggt ccacgggcgg atgggaggcc ctcgccgtcc aggtctttta
    15841 cccggacgaa tacaatggct gttatgcggc ctgcccggat cccatcgatt tcagggccta
    15901 ttgcctgatc aatctttatg aagataaaaa cgcctactac tatgaaggcc cctttaagaa
    15961 aatgctccgg ccgggagtgc gcaattacct tggccagatc tccacgacgg ttaaagacat
    16021 gaaccagcgt gaactggtgc tggggagcca tagccggagc ggcgaccagt gggatatctg
    16081 ggaggcgacc tactccccac agggcaggga cgggtacccg gaaaggattt tcgataaaac
    16141 aaccggggtg atcaatccag ccgtcgcggc atactggaag gaaaattatg atttatcgta
    16201 tattctcaaa cgggactgga agaaaaacgg ggcaaaatgg aaaggccgga tccaccttta
    16261 ttgcggggat atggacaact attatctaaa caatgccgtc tatctcgccg aatcattcct
    16321 tgaggccacc acggatcctt attatgacgg agaagtcgat tacggggacc gggccgaaca
    16381 ttgctggaac ggggaccaga cccagcccaa tgcaatttcc aggctcaggt atcaccgtta
    16441 tttcatcccc aaatggacct cggaaatcca gcaccgggct cctgccggcg ccgatctgaa
    16501 atcctggcgg tactgattct taccctgcca ttcgatttaa tcaggggcaa cccgggctta
    16561 cctgttatat taaaaatttt aataatatgt ggcatgcttc ttgttttatg atttccgttc
    16621 ggaaaatcaa attacatggg tgcctttacc gtcatatatc atttaatttt ttatttatac
    16681 ctgctgacct cttccgtctg gcctagcgat aattacccga tttcgcaggc ggcagggatg
    16741 cggatcatta cgatcacttg ccccccgaac gcattttcca gttgtcctga agaagagcct
    16801 cagcctttta gttcttattc cgaatttatc ctggccggcg gaacggtcag ttccaactgt
    16861 ggcatcgata ctgccagttt tagtttatcc cgggaggtag ttgttttccc ttcctgccca
    16921 agatccattc agcgcactta ttccgtgcgg gattcctgtg gcggccagga ttcctgcacc
    16981 tatctcgtgg tcatcaatga caataccacc ccggtgctgg tatgcccttc caccctggta
    17041 ccctgtgaca acaacatttt accaccgccg tttgccagtt ataccgcctt tttacagggc
    17101 atgttgtcca ttggcggatc ggtcacggac aattgttcca tcgacaccgc cagctggaga
    17161 tatatcgggg aaagcgtgga gcaaaacgaa aattgcctga ccaccgtcgt acgcacttac
    17221 ctcatcgagg attcctgtaa caattcggcc ctctgcaatc agaagttcat atatatcgat
    17281 atcacctttc cttcgttcac cccagcccgg gatacggtgc tttacctgga tgccaattgt
    17341 tttgccgata cctccgtcgc gaacctcggc ggcctttcaa atgtcgcgga caattgcggg
    17401 atattggaaa tcaccaaaca ggacatcgta cggcccggct gcaccgatct ggacacgctt
    17461 taccggatct ggacggtcag ggattcctgc cacaatgccg tttccgacac tcaattcatc
    17521 cgcgtctttg acaccatcaa acccctgatc gatgggccgg cggataccag ttacctttgc
    17581 atcagtgaag tgccgccacc cgatacggca tccctcgttc tcctatccgg ctgctcagct
    17641 ggggattatt ccaaaacggt ccgcgattct gtggcggaca gcctttgcct caataataaa
    17701 gtccttttca ggatcttcac cttcgctgat ggctgcggca acaccatcag ccatgtccag
    17761 gtcatcacca taagggatac cctacccccg gtccttgact gcccggacag catccgggtg
    17821 gcttgtgact cggagatccc ggtgcctgat ccccaaaccg aagtcaccgc tatagatatg
    17881 tgcgacggct tgattgatgc cgttttcatc agggattcca tcgaaaacga agcctgcgat
    17941 catcaaaaaa tcatttaccg catttatgcc gctgcggacc tctgcggcaa taccagccgc
    18001 tgtatccagg taatcaccgt acgcgatacc ttgccgccgg tcatgacttg cccggctgat
    18061 acccttgtat cgtgcatggc tgaagtaccc caggccgaca ccgccatggt ttcggccaca
    18121 gaccaatgtc aaggaaacct gcggatctgg catgataagg attccaccgt caatgaaatc
    18181 tgcgcaaaca aaaaaacaat ccttcgcatt tatctggccg ccgatgcctg cggcaatacc
    18241 agccggtgca tccagaccat cctggtcaat gatcagaccc ccccggaaat catttgcccc
    18301 ggggacacga ccgtcccctg tctgtccgag gtccctccgg tcaataccgg catggtcacc
    18361 gcgaccgatg attgcgggca aagtccggac atcctgcata tccaggattc catcgctgac
    18421 ccctattgcc ccaaccataa aacgattttc aggatctatc aggctacgga tgcctgtggc
    18481 aacagttccc ggtgcgtaca gatcatcacg attttcgata atgaacctcc cgtgatcatt
    18541 ggtcagtcca ataccatcaa cctgagttgc gacgaaacgt ttaccattca aatcccggaa
    18601 gcctttgacc tatgccaggg gatcgtaccg gttcaggtgg acagcctggt cacggacagc
    18661 ctttgcccgg gtacctataa gttggtcttt tccttcaccg ctacggacag ttgcggaaat
    18721 accggaaccc gttttgacac ggtcagtttc agggatgata ctcctccttt tatcgaatgt
    18781 ccaccggatg ccgtgatcaa ttgtacagag ctcaccgtat tcaccctcga ttcattcctg
    18841 tattacggcg gacaggtgag tgacgaatgc ggaatcgact cgtccagctt catgttcatt
    18901 ggtgaagaat ttacagagga aggagaaaat ttgatcttca cccgcaagtt taagatctcg
    18961 gacctttgtg gcaatacaga cagttgtgaa taccggatta ccacctcccg cgagtgtttt
    19021 gtggacctgg ccctgaaaaa aaccatccct tcttccagtc agcctttttt tgcccaggcc
    19081 ggcggattgg tacccttctg cgtcacggta tataaccagg gttttgtggc ggcagacagc
    19141 atccgggtga tcgactatct gcctgatccc ggaagcaaga tcattacccc cgggtggatt
    19201 gaccaccaaa atgggaattt ctgtatttac ctgagccagg ctaatcagcg gttgccgaag
    19261 ggagggctca agcccggcga cagtctgacc ttttgctttg aaatcaggct cagcccgggc
    19321 ctccgcgtga gcgaactggt caatatttgc gaggtcagcg agaccaggga tacttctatg
    19381 gcattgttgc aggacctgga cagtcataac gatgaaatta aaagcaacga tacaggtggc
    19441 gagcccggca ccgcaaacga tgattttatc atgggagaca gccggatggg ggaagatgaa
    19501 gacgatcacg atccatccct cttttatata tgccagccgc tgaattgtat caacaaaacc
    19561 aatgcctccg ttcaggcgga tccgggatgc ggccattgtt ttaaagccag tgaactgctg
    19621 accggggttt tattgccgga cgaattttat gttgtcaccc tttatgactc caaaggcaag
    19681 ccgctgccct ccaattgtgt ggggcgggaa tacctgggtc agacgctcac ctataccgtc
    19741 tccctgcccg cctgcggcca aaactcctgt tggggcaagg tgaccattga agacaaggcc
    19801 ccccccaccc tcgactgttc acccgatacg gtatattgct ctcaagtcag cgcccttccc
    19861 ttgcctggtc cggtccccga caattgcagc gggatggcat ccgtcaccct ggtcaccgaa
    19921 tcatggaaag atctgggttg cgccggcggt gaaatccagg gcatctatac ccgtcggctc
    19981 acctcgcgcg atgtttggaa caattccatt acctgtgaca aaaactatta catccggagg
    20041 atcaaccttc aggatatcgt atgcccgcgg gatacccttt tcgaatgttc cgacactgcc
    20101 gacgtcaccg atccgttggt cagcggcact cccaccatag acggcatagc cctgtggccc
    20161 ggcggggtca cctgcaaact tttcgtcacc taccgcgacc agagaaagga attttgcggc
    20221 ccgggtttta aaattgtgcg tacctggatc attggcgacc attgtacggg gcaggaaata
    20281 aaatgcatcc agaccattgc ttttgaggac cgcacccctc ctttagtgac cgccctcaac
    20341 cggaatgtgc ggcttaatgc ggacccgcac gaatgttcag ccttcctgga tatccccaaa
    20401 cccgaggtaa tcgattgcag tcccgtgacc tattcgtata cgatcaccta ttttgaccct
    20461 ttggattcca tcaaagcaag aacggtcacc ggaccattgc ctgcccggat ccggatcccg
    20521 gcaggtaccg aacagaaagt ttatctccat atggtggatg aatgcaactg gcatacccgc
    20581 gattcgatca ccgtgcaggt ccgcgacatc acgcccccca acccggtttg caaagcggtg
    20641 acccaggtca ccctggaccc ggattcctgc tggggaagca ttgaagcaaa aaacctggac
    20701 aacggcagcc atgacaattg ttgtcaggac ctgcatttcg ctgttgcgta catggatgag
    20761 atcgaagcgg cccgtaaatc ttttctggat tcccttcagc gcgcctgtgg taaaaatgaa
    20821 tacttaaaat acaaaggctg gtatgatgcc tacctggagg attggatcaa ttcctatgtc
    20881 ttccgggaca agttggacct caatgcctgc ggaagccgcc aggtcgtgct ccgggtctat
    20941 gaagcctgcg gcctgccgaa atacgatccc catgtttttc cctgcagccc tcatgcctgg
    21001 tactgttaca atacctcctg gcgctaccgg gtgaccttta accaggcttt cagaaacgga
    21061 gccggaacga gcaaagactg tacggtcaag gccccctggg gctgcaaatc cgacctggtg
    21121 aataaattca gttcaatgaa tgctttccag gccaattttt atgagtcggt atggctgccc
    21181 aggacttgcg acaccctcct tggtaatttc tccgcaccgg tggtttgcca gcccaggtta
    21241 tataatgact gcatggtcca ggtattggcc gatgataaac aaaaaccggt atgcgactcc
    21301 ctgcccgacc tcgaggtcta ttgcgacggc gtgccgggcg gttgggctta cgcctcggca
    21361 ttttgtccgg gcagtggcga caaatatacc acctacccgg gggccatcac gcgcaatgga
    21421 cagggcacgg tctacggcta ttatggagga agttatagcg caagccatga tgaacaccag
    21481 gtcttggata cggcctgccg gtcaaaccac tataaggacg gctggcagcc ggtgtattgc
    21541 agggcctggc tggaacttga ccgctttgac accctttacc ggcccgatcc caaacagttg
    21601 ttttaccaac ccgtttggac ggataaagca catccttacc ggagcctggc ggccaaagag
    21661 ttcaggatta cggacaattg ccttatcgac acggtctttt ttgaagaccg gggcaatatc
    21721 aatggttgcg gtgaaggttg gctggaaaga gtctggacca taagagaccg gtgcgggcat
    21781 accagcacct gcagccagaa aataagagta aaacacagga gtgattttga agtccttttt
    21841 cctgaagacc gggagatcgg gtgtacagac ctggctctgg ccgacccgga cctggccgga
    21901 aggccgcaaa tctcagatca ggattgcgaa caaattgccg tgcggttcca ggatgatacg
    21961 tttgagtcgg tggaagaggc ctgttttaaa attgtacgta cctggaccgt cattgacggt
    22021 tgccggtatg agccttccga tcatcaccag gactctgagg tgattgtcga tgaccggctc
    22081 agggccaatg aaaacgaccg tttctgtgtt ttcaggaatc tgaaggataa tggagatggc
    22141 atcgttcaat atatccaggt gatcaaaatc gtagaccata ctccacccca ggtcgtgtgc
    22201 atcgatacga ccctctgtct gtccggtccg gattgtgctt tactggtgga cattcccctg
    22261 gaagctaccg atgattgcgt caccgacctt tggtttgacc ttgcctttga ccataatcag
    22321 gacggaatat ttgatgatga aatccggaag ggggtccgga gcatcaccgg ttcatttctg
    22381 cccggtgaat attcgtttaa aatcacggcc tacgaccatt gcgggaataa atccgaatgc
    22441 aacaacaggc tggtcatccg ggattgcaag ccccccaccc cctattgcct caatggggta
    22501 atcacggtga tcatgcccag taccggggca attgaagtct gggcttccga cctggaccgc
    22561 ggcagttacg acaattgtac ggccccgcaa caattaaagt tcagttttga tcaggctggc
    22621 acaaatcttt cgagggtctt tagctgcaca gatatcccgg atggaaaaca gtcgaccttg
    22681 ccggtggaga tctgggtgac cgacctggcc ggaaatacag aatcctgcaa gacctttatt
    22741 ctgctccagg acaacgggga cagtcccggc ggagcctgca gggatagttc ttctttaccg
    22801 ggcctggttt ccgtcaaagg caagctggcc actgaggaaa aggaaccggt cgaaaacgta
    22861 gagatcagaa tgttaaccgg tacgaacacg atgaccaggc aaagtaccgg gccggatggt
    22921 ttctatcatt ttccggacgt tcagacccag ggcaccatgg aaatcagcgc acaccgggac
    22981 gacaacccca tgaatggagt gagtaccctt gatctcctcc tgatcgaaaa acacatcaaa
    23041 ggggaaaggc cactgtccag tccatttaaa aagattgccg ccgacgtgga caaaaacaag
    23101 gaaatcaatg tgctggacct ggtcgaactg cgaaaactca tcctgggtat atacgaccgg
    23161 ctgccgtccg gagaaagctg gaggttcatt ccggcaaatt attccttcaa agacccgggg
    23221 caaccttttg attatccctc atccgtgcaa ttaaaagggg accaggacaa ccggcttgac
    23281 tttacaggga tcaaggtggg cgatatcaac ggaacggcgt ctccccattc ccttaccggc
    23341 acagagatca ggcaggccgg acccggtctg attttcacca tacccgaccg gcaggtccgt
    23401 cagggagaat ggatagaagt ccctctccgt tcacctaatt tcagcggcat ttccgggttc
    23461 cagggtacgc tccattttga aggcttggat taccagggcc tgattgcggc ctccctgccg
    23521 attaccattg aaaacaccgg ttaccgctgg ctggaccaga aagacctcac cttcagctgg
    23581 catcacggcc gttcaatgga aataacggaa ggccaaaccc ttttccttct tcgcttcagg
    23641 gctttgaatg acctgaaact gagtgaatcc atctggatca attcccgcca tacggtcgcc
    23701 gagagttacg aaggccgggg cgaagtcaag aacctgtccc tgcagtttca aactgcttcc
    23761 ggcaggctgg ccttggcgga acaacggctg ttccagaatt atcccaaccc cttttcagat
    23821 gccacggtca tcggcctcag cctgagggaa aacggtaaag gaaaattgaa cctgctggac
    23881 gttacaggga aactgatcaa aaccattgaa aaagactggg aagccggata ccaggagatt
    23941 cggctggagc aaagagattt cccgggaccg ggcatctatt tctaccagtt tgaaagtcca
    24001 ttttttaatg cttcccgaaa aatgattctc agaaattaag cgaatagatt tgattttcga
    24061 acggagccgg ggggttgaag aatccaccgg ctttattttt ttatcgcagg ttgggcaaaa
    24121 ggcccatctg ccgaagcatc gatgcttcat cccaactgta aaggaccttg atgatcttac
    24181 catccttcac atggaggata gagatcccat agaattcaat agggcggttg gtggccggga
    24241 tattcataaa cgggccttgg tgggtgcctt tcgctttcca cacgaggaca gtttttccct
    24301 gcttgcccga aatttcatcc ttcagggaaa attgcaagtc gggaaaggcg gtccagtaaa
    24361 ggtccagcat ttggctgact gcggcgacgt catattgccg gttggtaccc aggtcttcgc
    24421 gtacaaaatc cgtggaatac agggaaaaaa taagggactt gtcccttgaa ttccaggcgt
    24481 cgatcacgga ttttccctgg accagggttt gatttaggat cgggtcgggt tgcatggatg
    24541 gctgctgtgg atataaatga agatggttta aatttgcatt tacaaaattg aggctaaaat
    24601 tgcggttccc cttactcatt ccctgcccag tttttattca ataattcatg atggctcaat
    24661 tcccagaaaa tcaagtacat acttccaggt tacgtctggt tccttttacc gagaactggg
    24721 tggcggccat ccaggaaggc agccaggctt tcctggcctt gaccgggtac cggctgcctc
    24781 agccctatac tgaatttccg gagtcttttc aggccctgcg caccgcgctt gacaaagaaa
    24841 aaccagtctt tccctggacg agttatgccc tgctttggga accggaaaaa acctatgtgg
    24901 gacagggagg cttcaaaggc aagcccgatg tggatggcgg ggtggaaatc gggtatgaaa
    24961 tcgcgaaagg attccgttcc atgggttttg cccatgaggc cataggtgcg ctggtccgtc
    25021 tgtcgtttga acaggagaag gtgcgccagg tttatgctca caccctacct gttctcaatg
    25081 cttccaacca cctgttgatt aaaaataaat ttgtgtttga ttcaaccgtc acggatgaag
    25141 aggacggcca ggtctggaaa tgggttttga ggaaaaccgt cttcctgctt tcagtggaat
    25201 agggaggcag ggctttctcc aggtcagttt ttttccattt ccatattcca gagcagtttt
    25261 accagctttt ccagtgcggt ttttaaatac ctgcgatagg tgctgaaaga catattcaga
    25321 aaatcagcca ccttttcctg ggagccgacc gggttgatga aggtgcgata cagcacgcgg
    25381 tggtatttac cgtcaatggg tgagtcttca atttctttca gggccaggtc gatgcgtgct
    25441 ttcaggattt tcagtttatc caggtctttc atgtccggca tgatcatccg caggaccagc
    25501 cttgacctca aaagagggct ggaaagcaac tgatcgggct gatgataata tttgacggcc
    25561 tgatgaacgg aatccccaaa ctcctcttcg ctcaggacaa gcatggaaac cgcctccgta
    25621 cttgcttcgt cgtattggtc caccagttcg acctcccgtt tacccaataa atccagccag
    25681 gccaggggag gtctttttcg ccagtcgtgc acataccatc caaaaggaat gccatccgat
    25741 acaaaatcga gctccggtac acggctgagg tcggcatatt gaagcacggt tttccaatat
    25801 tccggttggg cgcaggacaa gaagctgact gccagtcccg gggtaaaata atattgtacg
    25861 atggaaagga aaataacgct ttgaaggccg gacacaccct ggtaagtccc gtcggccatc
    25921 cagaaacgga aggcggtgaa aaggtcaccc ggcctggggt tcatccgggc caggacatat
    25981 tctgccactt ttttaattgc agggtcggga agctcatttt gttctttcag tttcaggatt
    26041 tcgattttca ggcaaaaggc caccgcctct gattttgaat tccggtatac ccaggtttcc
    26101 gaggctgcgt gatttatcca ataattgaaa tataactccg cttcaggccc ttcccatttc
    26161 ccgaccaggg cgcggagtac cggaaggtcc cccggtttga gttgatcctg ccagaaagtg
    26221 ccgttctcct gccagtcgaa aaacggtttt accattggat tgagccggtg aaggtaaatc
    26281 aaagaaaaaa gcacgcttcg ctgggcctgg tgggaggtgc tgttcagctt ccggatataa
    26341 tagttgcgta ttttttcatg aaggctgtgg taccaatccg ggttcctcca ttttacatcc
    26401 cggctcagag cttcccgggc gatatcgtgg gggaaaatgc ccgattcatt tttgtcaaaa
    26461 aaagacagat tgcggagcca ttcaaaaata ccctggatgt gttccatgcc cagggcttcc
    26521 tggaggaggg actcggtggt gacatgaacg agggagctga tttcaagggc tgcgcggtgg
    26581 gccgggccgg gcacattttg tacaaaaagc tcgagcaggg tcttgatcag gtcggggctt
    26641 cgctcgggat taaaatcctg ccccgggtgc tggtcaaaga gatcagccgc caccgacagg
    26701 gccagcgggt ggccatgggt gaattccaat accgcgggaa tcgttttttc cggtatgttc
    26761 cttttgatta aatactgcct ggcttgggat gggctgaggt tgcgaacctc caccgctttc
    26821 atatattttt tccagccggg atcgatggcc caggaagtgc ccgggggatt tcggctcagg
    26881 atcagggtgc agaagtgagc aggcaactgg ggtaaaaaat cctggcggat ccagtcatcg
    26941 aagggatgca gtctttcgaa ggtatctata aacaagacat ggctttcctg cagtttttcc
    27001 aatgcttcaa atacatcctc atcgggtttc agttgaagcc tcgcatgcaa agcttccgta
    27061 aaaccggccg gatgggcgtt gatctcccgt ccatccagat gaagataagg aacgtcctcc
    27121 cgggcgcaca gatccatgca ttttttcatc agggtcgtct tgccctgccc gcccgggcca
    27181 tggaaataca acaacagcac cgggttttcc ttttttaaga cggactggaa cagatcgagt
    27241 tcggcatccc tgcccacgaa cgcgcttttc ctttcgtgtt ccaggatttc agaaagtttg
    27301 gcacacatcg aaggaataag ttagaatgcg ttctgccctg taaattaaga aaaagccaaa
    27361 aaatccgatt tatacatgta tttttcactt ggatttttca ccgacttatg acccaaaaaa
    27421 ttagctcact gcacaacagg ttgaggcagt ttgccgatca tttgattgaa gaggaaatga
    27481 aggtttatca tgaattgggc atcgatattc ctcaccgttg gcatgccatc cttcaaattt
    27541 tcaacgagga cgatcagccc cataccatca ccgacctggc cgaacttcaa aacaaaacac
    27601 atccggatgt ggtctatacg gtaaaccaga tgatgaaaca cgggctggtg cgggaaatga
    27661 ccgaccgcaa cgacaaacgt aaacggttca tcaaagccag tgaaaaggca aaacaactga
    27721 tgagcgaatt aagaccggcc tggcaggccg tgcaggacgc caccaacagc tgggtcaagg
    27781 aaatcgctcc ggacctctgg ctcaatatcg aagccctcaa taattccctg gagaaaaaga
    27841 gttttttcca gcggatcaaa aaagagaaaa aaagggcgga tctgcgcaat gtcctgcttt
    27901 cgcggtttga ggaggtgcaa aacggaaaaa tgctgctgca gaatttctgg aaccagttta
    27961 aaggcaaata ttttaacatc gatgcccttg atgaatttat cctgaatatt gacgaacaca
    28021 tcaccaaaaa ccaggcccag gtttattttg cccgctgggg agaaccgatc atcggctgca
    28081 tctgtctcat ccgccggagt ttcaagtttt gtgagatcgt gcacctttgg gtggatgaaa
    28141 attaccgcag gaggtatgta ggcaccagcc tgctcaataa agccattgcc gccggaaagg
    28201 aaatgggagt caatgccctg ttttctcaaa gccatcctaa actggtggcg gccaactctc
    28261 ttttccagca tgccggcttt tcagtgagcg accaattccc aaaggcggca gagg
//
LOCUS       DAOQXT010000060        28096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1964_length_28096_cov_6.545808, whole genome shotgun sequence.
ACCESSION   DAOQXT010000060 DAOQXT010000000
VERSION     DAOQXT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 28096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28096
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1964_length_28096_cov_6.545808"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKM27_11315"
     CDS             complement(<1..330)
                     /locus_tag="PKM27_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07895.1"
                     /translation="MNQDILHAHTSRGDFTAGQLDALEVSLLPDQSYLVMVNNKPIIV
                     RIESCGSDLRNFTVRVGRDKVEVGLVREIDQLVQSMGFNKSVRHWVETLRAPMPGMVK
                     QVLVQEGE"
     gene            complement(327..710)
                     /locus_tag="PKM27_11320"
     CDS             complement(327..710)
                     /locus_tag="PKM27_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07896.1"
                     /translation="MSIRQFYIQLAFITGLTGMTLYFLTRYSGFESGWFPLVGLIFFF
                     LFSILIFHLGLHSAGHQDKNRFTGVVMGMIFLKLVLTLGIVLVYDKMIAPLGIQHVLS
                     FLIAYLFFTFFEVYFMSKLARRRPA"
     gene            complement(711..929)
                     /locus_tag="PKM27_11325"
     CDS             complement(711..929)
                     /locus_tag="PKM27_11325"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNR07897.1"
                     /translation="MTQPENPRKPAGEILKYSGMAFQMAVYLVAGWWLGSFADRYLNT
                     AKPYFSLAFAVLFLSAYFVKLIRDLSKK"
     gene            complement(935..1381)
                     /locus_tag="PKM27_11330"
     CDS             complement(935..1381)
                     /locus_tag="PKM27_11330"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNR07898.1"
                     /translation="MFGSKKTESTTSTPVKSISSNAGSSALNSVVSGTRIEGKITCDN
                     DIRIDGNLKGSLICHSKVIIGPSGVVDGDIECENAVIEGHFTGILTVRDSLQVKETAN
                     IKGDINTDKLVVHAGAVFNVSCLMKNEEHQVVTSSSNGIYTEVDQV"
     gene            complement(1509..2543)
                     /locus_tag="PKM27_11335"
     CDS             complement(1509..2543)
                     /locus_tag="PKM27_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNR07899.1"
                     /translation="MKKACVLVLMLSCIYGTAQDIHFSQFYMSPIHLNPAMTGVMNCN
                     KRFIGNYRNQWSSVLRSNAYNTFAASYDQRIEVGRYDYFGIGGGFWGDVAGQLNFGTI
                     QGKLAGSFSKRMAGSRTNSHYLAAGFDVGLAQRSIDFTKAQYGSQHDGQGGFDPTLPG
                     EALDRDNFVFADLNVGLLWFSVLDKNNFYVGAAYSHLNQPNQSFSNDRLVPLTSKITV
                     HAGGEMALKNNLSLVPGLVMFRQGEAFELNLGTSAKFITQKAKDFEQSFQFGAWVRLA
                     NHFEKTFTTDAVIVSSRFDFNQYGIGLSYDLNISSLRPASNGNGAFELSLIYNICDGS
                     SRRGVYCPTF"
     gene            complement(2613..3395)
                     /locus_tag="PKM27_11340"
     CDS             complement(2613..3395)
                     /locus_tag="PKM27_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07900.1"
                     /translation="MKPFWLGLFTLGTAFMVRAQTTIQPRQMDYGYSGVLYTSEYTLQ
                     ADLQTNGFKLGYRKGTIKKYYLTTFYHLDFGTLKHPKEYRQSLRTQSILHNITSANSF
                     IYGKQNSFMAVRGGIGGKRYFSEKAKRKGISVALAYEGGPSLGFTKPYYLDIRRYNGD
                     RYFVQAERYSEENREDFLDLNSIAGSASFLKGITQLRMIPGIQGKAALNFTFGDVEEY
                     VKSVEAGLMMDLYLRKIPIMIEAPNSAYFINFYLGIQLGKRK"
     gene            3518..4678
                     /locus_tag="PKM27_11345"
     CDS             3518..4678
                     /locus_tag="PKM27_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNR07901.1"
                     /translation="MQTNARTIWIVILSALGLYLLYYFSDLVSFILASWVLSMLGQPI
                     MKLFLEKLKLNKFSYGTSVAAVMTILLYILAILLIGVLFIPIIIEQASHLANIDYDKI
                     GASLQPPITYINNELQSLGLISSEQSVMDQIFEVVQKWFRPERVSNIFGSIIQIGSNL
                     TLAVGSIIFITFFFLQESGLFQEYLVAIVPKRHEEKISHILSDASRLLRRYFSGILIQ
                     GSIFCLISYIALTLFGVRNALLIGFFGGLMNVIPYVGPIIGMIFAAFITLTGYVGADF
                     SQVILPLCIKAIGSVFIAQVIDNWLVQPFLFSKTVKAHPLEIFLVILIAAKIGGITGM
                     ILAIPAYTVLRVVAKAFLSEYKIVKKITEDMADQPLEIPEEAPADEAANDKP"
     gene            complement(4694..6022)
                     /locus_tag="PKM27_11350"
     CDS             complement(4694..6022)
                     /locus_tag="PKM27_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HNR07902.1"
                     /translation="MQPKTITGFSKLSKLGKIKWLVENFFKDPENVMHELKSYWLDNQ
                     EHQELLDGISENTISNYPLPLGVVPNFVLNGKTYCVPMVTEESSVVAAASSAAKYWME
                     RGGIQAVIINNLKIGHVHFYWTGSPERFGELFELLKPKLLEESRGLMESMLKRGGGLK
                     SLTWKSFTGREPGLYQLFAEFDTRDSMGANFINTVLEQFSSTLQEFFKSDPALDGDER
                     EIDVIMAILSNYTPDCLVRTWVECPIADLGDFPGRMDALTFAERFYHAVRIAQVDPYR
                     ACTHNKGIFNGIDSVALATGNDFRSIEACGHTYAARTGKYQSLSHCAIDQGMFRFWME
                     LPLAVGTVGGLTSLHPIAKRSLEMLENPGAGDLMMIIAATGLIQNFAALRSLVTTGIQ
                     QGHMKMHLNNILTQTGASPEEKESAAAYFLDKTVSVNSVKLYLESIRKSG"
     gene            complement(6038..7060)
                     /locus_tag="PKM27_11355"
     CDS             complement(6038..7060)
                     /locus_tag="PKM27_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate delta-isomerase"
                     /protein_id="HNR07903.1"
                     /translation="MEEKQSPQLTDQRKKDHLDLALQSRMNALQADERFYYEPLLSGH
                     PSHSNIPETPWDGKVLKNPIWISSMTGGTAEAVVINERLARICKAFGLGMGLGSCRIL
                     LDSDQYLSDFKVRKFMGDQPLYANLGIAQVEQILLQEAWDKIERLIERLEADGLIIHV
                     NPLQEWLQPEGDPILHPPLQTIVSTLQNIKAPLIVKEVGQGMGPESLEALLKLPLAAI
                     EFAALGGTNFSTLEMKRRPAGNTGEYDNLSRVGHTAGEMTRFVNEIVSRLGDQVKCGS
                     VIISGGIKDFLDGYYYMQKLRFKSVYGQASGFLQPARKSYDALYDHARGQIDGLQMAY
                     GYLRVR"
     gene            complement(7129..7959)
                     /gene="tsf"
                     /locus_tag="PKM27_11360"
     CDS             complement(7129..7959)
                     /gene="tsf"
                     /locus_tag="PKM27_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNR07904.1"
                     /translation="MSEVSAADVKKLREMTGAGMMDCKSALSEAGGDFEKAIEILRKK
                     GQKLSVKRADREAKEGAVIALVSPNRSKGVVIRLSSETDFVSKNEDFINTARQIAQVA
                     LEQFPPSLEELLSLKLNGVSIGDKVTDMVAAIGEKIGLESYERLEAPLVVPYIHMGNR
                     AGVIVGLNKAGDQYLEAGKDVAMQIAAMKPIAVTPDGVDKSVIEKEIEIGKEVARQEG
                     KAEEMLEKIALGKLNKFYKENTLLQQEFVKDSKLNIDQYLKKVDKDLTVTAFKHVMNG
                     "
     gene            complement(8022..8759)
                     /gene="rpsB"
                     /locus_tag="PKM27_11365"
     CDS             complement(8022..8759)
                     /gene="rpsB"
                     /locus_tag="PKM27_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNR07905.1"
                     /translation="MKIPTYQEMLDAGVHFGHLRRKWNPKMRQYIFAEHKGIHIIDVN
                     RTAENLEKAASAMRNIVKSGRKIMFVATKKQAREIIANAARTVNMPFVTERWLGGMMT
                     NFATIRRSIKKMHNIERMLAAGNELSSITKKERLTLDRERAKLDKELGGISQLNRLPD
                     ALFLVDIGHEHIALQEATRLGIKTFAMVDTNCDPNLVDFAIPANDDASKSISIITSVL
                     VEAIKEGLEERRQMKAELEETGAAANA"
     gene            complement(8791..9180)
                     /gene="rpsI"
                     /locus_tag="PKM27_11370"
     CDS             complement(8791..9180)
                     /gene="rpsI"
                     /locus_tag="PKM27_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNR07906.1"
                     /translation="MEIINGVGRRKAAVARVYLSKGTGQIRVNGRELADYFPLRNMQA
                     KLLEPFQAAKVEQNGFDMKVLVSGGGFKGQAEAIRMGISRCLVKINGEHKKPLKDLKL
                     LTRDARVVERKKYGKPKARKSFQFSKR"
     gene            complement(9206..9631)
                     /gene="rplM"
                     /locus_tag="PKM27_11375"
     CDS             complement(9206..9631)
                     /gene="rplM"
                     /locus_tag="PKM27_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNR07907.1"
                     /translation="MSAKKEDFTGKRKWFIVDADGLTLGRLSTKIASILRGKTNPAYT
                     PHVDTGDFVIVTNADKVRMTGKKMLTKEYQSYSGYPGGQKTKTAKELYARKPHYILEH
                     AVKGMLPKNSLGRQMFKKLFLYSGSEHPHQAQKPETLTL"
     gene            10134..11840
                     /locus_tag="PKM27_11380"
     CDS             10134..11840
                     /locus_tag="PKM27_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNR07908.1"
                     /translation="MYFNSDLQAENTYDAIVVGSGISGGWAAKELTEKGLKVLVLERG
                     RMVKHGEYPTAALAPWQLPNGNRVTREEAKDYEKQSRTGYTISQATKHWFVKDTEHPY
                     SETKRFDWIRGYHVGGRSLMWGRHSYRHSDIDFTANAKDGHGTDWPIRYKDIAPWYDY
                     VESFAGISGMKEGLEQLPDGKFLPAIELNCVEKKVRESLMVKYKGARVLSSGRVANLT
                     QEHNGRSKCQFRNLCSRGCPYGAYFSSQSSTLPAAEKTGRLTIRPNSIVNSVIYNPET
                     KKATGVRVIDAESKESMEFQAKVIFLCASTINSTWILMNSATEIYPDGLGSSSGELGH
                     NLMDHHFRVGASGEMPGFEDMYYFGRKPGGIYLPRFRNVGQDKQKDFLRGYGYQGGAS
                     RTGWQRSVKELSFGEDLKETLGMPGQWTMGLGGFGECLPYHENKMTLNKDLLDKWGQP
                     TVTFDCEFKENEMNMRKDMGSSAAEMLEAAGAKNIRVNMPNDNAPGLGIHEMGTARMG
                     TSPKNSVLNAHNQVWDCLNIYVTDGACMTSASCVNPSLTYMALTARAADHAVNELKKM
                     NM"
     gene            11874..12443
                     /locus_tag="PKM27_11385"
     CDS             11874..12443
                     /locus_tag="PKM27_11385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HNR07909.1"
                     /translation="MDRREALRISALLMGGSLSAATIAGVMNGCKASTVPDWTPAFVS
                     KELANSLAELCETIIPAGKTPGAKDAKVERFIDVMMKEVFKPEDQETFTKGLKQLDSD
                     ARSKYGKAFDKIKPEERVEMVKAMDDELRERKEQPASKPFYDLVKELTVTGYCTSEPG
                     ATQHLKYVAVPGDYKPCIPLSEVGGTWAI"
     gene            complement(12513..15242)
                     /locus_tag="PKM27_11390"
     CDS             complement(12513..15242)
                     /locus_tag="PKM27_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNR07910.1"
                     /translation="MDHLLKLINEAQENGNYPISITLCQDLLKQASQSAGPIGSRFHN
                     LAELYLTEAYRELSLDSLLEDQLNAIRESLDRRSGEAHSFQTAVRQFAYYIYYADFLE
                     DKKKVDQAVSQLNAAIKFLLDHPQFYGEDPIRGSSESLAHRLFQCYRRLGKMAMDAHE
                     YVEARNYFYEALKQARKADQVFSRTGPLTQESEEIALEKLLDLAQKSGDRQLYDTYLL
                     QYQNIQKSSAYSLTREKDYLFAQLQSDALGWEEGLKLVSRMDLNALVSEDLTVQFAKL
                     DLFLKFNRRDSCIPILARLEKQIRHPVDLAAYFRAKAGLAVTSGDFLAMSDAMDSALY
                     HLSIHRSSDSLWGDLGLRTQLLETVMKGIQYHEQGYVQCRDAFFLEKKTKLLKHFITG
                     LQSIRNDLISDEDRLSFVRRLMPILDLALETYTDPSTPYVPDYESVLTCFEAGKSFNL
                     HSEYGLRKSISHQDLIKFTRLSSELREVKTQLELGGPAYDSLLGRHNSLQMQVRDLKR
                     MYTRQPEKRWGSLSHLQSLLDQETTLLEYFKGKSRIYALLINADHIGLYRLPGHPDTI
                     GQDINDLRLSISMAHESRYTRLRDSLYRQSALKLFRGLLQPIKPYLKRRLVLMPDLEL
                     ARLPFGILLTEEVGDSDYKKWPYLVREHVISSQYSLALWLDQTRKTDVGKQGSGKKSL
                     VSFAPVFHDLTYNREETKEISSLLGRASDFFGPEANRANFMKFAGQGRILHIASHAYS
                     NEDQFGESFIRLQEDTLLAGELGVMHLPQDLVFLSACESGTGKYVSGEGMMSLARSFF
                     QAGSRSVISTLWPIRDDLSKEQVVRVYRFLRAGQPKDEAIRNMQLNFIRNARFGQAFP
                     AFWAGYQSQGSQAPLFPNLLISLGYILGLAPFLALGLAFLFYRKYIKVSKLAS"
     gene            complement(15479..15817)
                     /locus_tag="PKM27_11395"
     CDS             complement(15479..15817)
                     /locus_tag="PKM27_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07911.1"
                     /translation="MSKSSDCRQSPPCYVMAAEIYQNSLLNDMVDFSNFDAEILDETG
                     KVLVDYDKRTTPVSLSPGAEATDTLYYLSPKPESESRVKAYRGTNVIIRIFSGPGSSP
                     LEIRLKVRRN"
     gene            complement(15824..16111)
                     /locus_tag="PKM27_11400"
     CDS             complement(15824..16111)
                     /locus_tag="PKM27_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07912.1"
                     /translation="MSLDYSKVFLVDELKFRAYGQLMEDSKLKDEPGIALHYSKLMQE
                     ALRQAELKRPALFTEESIEASFGTVKQIYNTGWFGRVMAFFLRTFGFNPHK"
     gene            16298..16882
                     /locus_tag="PKM27_11405"
     CDS             16298..16882
                     /locus_tag="PKM27_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR07913.1"
                     /translation="MAKETDEELVNMIKSGGPAREKALHRIYTDSNIREKIIAMITNN
                     NGNQQDGEDMVQEAIIVMDKNIREGRYRTEGNLQSYLFAIGKYLWMNQLRKKKPMLKG
                     SFSDLEMTGDPDEPDHFLLDEERKMKLQAILSRLGNRCQEILSLWQLSFSMEEIAAQL
                     KLNDASTARKAKYDCQQQLIKLIQKSSLTKSDLT"
     gene            16879..17670
                     /locus_tag="PKM27_11410"
     CDS             16879..17670
                     /locus_tag="PKM27_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07914.1"
                     /translation="MNQIIPDEALLDQFEAYHSGRMNTEDQNRFANRLATEPGFNESY
                     HGFLTSREAIEIKISDDLKAKMKKWQKQSLPGGLSEAGRIRRLPWSAWAAAAALLFLL
                     TFGFIQYRSVQSYPLDAIDEYQPVQALTLRNGDQDSGLNGIIRDYTSEAATLDQTISA
                     LLKIRPETPGNEYQKAQSYLAKLYLRQGLCADAAAAYLRSGELEGARDEAEIGAVLCL
                     IREGHTREEINGALKPILDHPNHSYALAAREIDQKINGFWWRLFK"
     gene            17730..18389
                     /locus_tag="PKM27_11415"
     CDS             17730..18389
                     /locus_tag="PKM27_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNR07915.1"
                     /translation="MFFPRICLACDKNLQARGHLFCIHCSYTIAITDQWKKPENEFTE
                     KFAGRLKLEQGAALYRFVPGGQLQQVIHKLKYQNRPDIGIHLGRTAGHQIHRYWPLPD
                     LIVPVPLHPKKYWQRGYNQAEQIARGIGEITGIPVLPNALEKTGYTRSQTRLHRLERM
                     QNMEKSFVQHPSVKMIGKHVLLVDDVLTTGATLEACALEILRNPGSRVSMCTLAMGRR
                     I"
     gene            18438..18944
                     /locus_tag="PKM27_11420"
     CDS             18438..18944
                     /locus_tag="PKM27_11420"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HNR07916.1"
                     /translation="MTATELVKKECLRRINESHEKIISSLAGFTQEEIWWRPNPASNS
                     MGNIILHLCGNLTQYVLSSLGGWPDQRQRSMEFEEQGPVPVKELFDKLEEVMRNVNET
                     IHACNDYELMRVRPVQCFEESGIGILIHVTEHLSYHTGQIVYFVKWKTGKSMHFYDGV
                     DLEARGEG"
     gene            complement(19017..19787)
                     /locus_tag="PKM27_11425"
     CDS             complement(19017..19787)
                     /locus_tag="PKM27_11425"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR07917.1"
                     /translation="MKKLFFFILPLALTFCKNKEMSDTGTAPAADTTAMVVDSNNMLT
                     DAEAASGWKLLFDGKSLDAWRVYNGDSIPHTWKVMDGQIVLKPEGPHGAMVGGDLVTR
                     DTFGDFELKFDFKLADSANSGLFYLVNENPKLAIWQTAPEFQLVDNNYVNTHEMEAAT
                     NHLTADNYDLMSCQGATFNAAGQWNTAGVIKKGNRVEHWLNGVKCVEYEIGSKEYTAL
                     VAKSKFKPFKGWSLNKTGRIGLQNHGGEIAFRNIKVRG"
     gene            complement(19861..20529)
                     /locus_tag="PKM27_11430"
     CDS             complement(19861..20529)
                     /locus_tag="PKM27_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07918.1"
                     /translation="MNLYANQLYHVYNQGNNRRPIFFSDENYQYFLWKMKMYITPFAD
                     IISYCLMPNHFHWLIYINRDLIERKSLRLYVNSIERQRREIKFGNVFSGIYTDRWEKS
                     NDHSKISINEAIGILLRSYTRAINKENGWTGSLFRAKCKSKNGWIDQFVTLKKSNGKT
                     DFRFTTGNDYAYACFHYIHDNPMVSGLVRETLEWKYSSARAYAGLETPDVCNVEMGRK
                     ILGI"
     gene            20657..23596
                     /gene="dnaE"
                     /locus_tag="PKM27_11435"
     CDS             20657..23596
                     /gene="dnaE"
                     /locus_tag="PKM27_11435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNR07919.1"
                     /translation="MYLNAHSYFSLRYGTLSPSELATGAAALGHTWMALTDINNTSCS
                     FEFLQACQKAGLRGLLGIEFRDPEHRYLYTGIAQNAAGWHELCALLTRSSLSGEPLPA
                     LAPPLEHCFVIYRSLPKPLADFAPHEYYGVSPAEVGGLYSSPLRKDPGRLLAFASVSH
                     RDREGHRIHKLLRCIDRNIVHTRLEAADTAPVTDSLVSRDSLLQAYAQYPELIRRTEQ
                     VADQCSISLESGTGVNRRTFTGTAGGDYELLTKLAREGCRRRYGTTHKRAAERTEKEL
                     QVIFKLGFAAYFLITWDVVRYARSAGYHHVGRGSGANSIVAYNLGITDVDPLELDLYF
                     ERFINPHRSSPPDFDIDFSWSERDDVTDYIFKRYGHEYTALLATYNTFKGRSIIRELG
                     KVFGLPKNDIDLIVDEPLASDKHHPWAKHIFRFGKSIENFPNYLSIHAGGILISERPL
                     NEHTALQMMPKGFAITHFDMYGAEDLGFHKYDVLSQRGLGHIKDAVDLIKRNKRQSVD
                     VHDIARLKEDPKVRAQLRSGHCIGCFYIESPAMRGLLHKLRCDDYVHLVAASSIIRPG
                     VAQSGMMREYIERFHHPEKIQYLHPVFEKNLKETYGVMVYQEDVMKIVHHFAGLDLDE
                     SDVLRRIMTGKKKSSDTFLRLRDKYFANCKARGYSEALTQEVWRQIESFSGYSFCKAH
                     SASFAVESFQSLFLKTYYPREFMVAVINNFGGFYNTELYVHEARMCGAIIHAPCVNRS
                     TYLTTLYGDDLFIGFVHIQNLEQNVAVRIVEERLANGPYRSLEDFVQRIDVSKEQLEI
                     LIRINAFRFTRMNKYELMWEKNMVHNPQVKYAQNGNFFPDAADQYSLPELDEGRFEQA
                     FDEIELLGFPLCSPFDLLAEPFKEDILARDMPAHHRRTVTMLGYYVTRKWVSTKNNKL
                     MSFGTWIDREGRFFDTTHFPPSLEAYPFKGKGVYRIRGKIVDDFDFKSLEVMDMERLP
                     YRKDERY"
     gene            23979..25151
                     /locus_tag="PKM27_11440"
     CDS             23979..25151
                     /locus_tag="PKM27_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07920.1"
                     /translation="MKKKLLINWLYYHPVGHVIEPLKLAKGYQLANKNLEVYLIINAD
                     SPRELPDACPWIKKTYTVSLDEVQRCGAGALQKIPKKWDYVITDNRARWLVSGRDNEN
                     LIHAQDLLKKLLVAKVKKGYVEQSGIQKESILPIVGNPKIKLPVPDKARDFAKKFKHR
                     GPKIAILLGGSSGKNRSPGLKSWLRICQALVAAIPDLKIYFTGVSRSINGRTATRDFN
                     REDVDDLINRLPGAEYAYDLGLWNQIALIETCDIFLSPHTGFAFIAPLVNTPWLEIAT
                     CPWPAYIFNDLPFYSVLPDCGSYPGWGNPLKGCGKLLQENKKARCVSDRSLKKKIPEI
                     VRGARYLLDKKFTYKKAISLHLKKIRKNYPIQHFFFLGGIEGLTHTPGEPIPKNQS"
     gene            complement(25155..26909)
                     /locus_tag="PKM27_11445"
     CDS             complement(25155..26909)
                     /locus_tag="PKM27_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNR07921.1"
                     /translation="MGRIKNLVPGLFLAFCLALGCDSRQDDQPNVVFILSDDQGWGDL
                     SAHGNPLLQTPNLDRLIQEGLEFERFFVSPLCAPTRASLLTGRYHLRTGVTSVSNGYE
                     VMDADETTLAELFKANGYRTGCFGKWHNGSHFPHRPEDQGFDEFIGFCAGHLTNYFNT
                     GLDSLSHTVKTQGYITDILTDRAIDFIGRNKDQPFFCYLPYNAPHSPFQLPDAWFDKY
                     KAMGLDDELASLYGMVDHMDYSIGRVLKSLEETGLEKNTIVIFMSDNGPNGVRFNGGM
                     KGIKGQVDEGGVRVPALIRWPEKIEAGRKINQPLAHIDWYPTLTGLCGLDPVMTKPID
                     GMDFSTQILGHHAEFPSRTLFTHVAFLNKNLISYPGAVRTATHRLVVRDTAHYLYDLV
                     KDPGQTTNLAPEQTELAGNLKNQYDQWFASVRQGYKPEKAIALNTSHILLPAYEAEYT
                     GNLRFAEGHGWAHDWLVNWSSTADTIRWLVESDTMRVFEVLLKYTCPPTDLGSVISVR
                     LGEQSIQATVTDSFDPPFLPSPDRVKRKEAYEKEWANMRLGAIRIPGGKHHIKLIPVS
                     VKHKRVSEVAGIELIEEE"
     gene            27020..>28096
                     /locus_tag="PKM27_11450"
     CDS             27020..>28096
                     /locus_tag="PKM27_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR07922.1"
                     /translation="MLNLSTLLQENSRRYPDKTAILFGESALTYTQVNGAANQVANGL
                     LQLGIKPGDKVALTCPNLPWFPIIYYGILKAGAVVVPLSVLLKKDEIAYHLRDSDSKA
                     YFCFTGTDEIPMGRMGLSAFEETEGCEYFFLIMPKPDMPPSIEGCNTLGSLLAGQSPH
                     FDTVQTSAEDTAIIIYTSGTTGKPKGAELTHNNLMHNAILCVDLLYLDKEDRLLIVLP
                     LFHIFGMTVLMNAGMFRGCTAVLLPRFDAGAVFGLMQKHAITVFAGVPTMYWGLLHHQ
                     DDNLDDDLIARNLKICVSGGASLPVKVLEDFETRFKVKIIEGYGMSEGSPVVTFNMLD
                     QGRKAGSIGKPVWGVDVKIVDEKDQ"
BASE COUNT         6990 a         7247 c         6953 g         6906 t
ORIGIN      
        1 ctcgccttcc tgaacgagga cctgcttgac catccccggc ataggcgccc ggagggtttc
       61 tacccagtgc cgaaccgatt tgttgaatcc catcgattgg accagttggt ctatttcccg
      121 gaccaggccc acctccacct tatcccggcc aacccgtacc gtaaagtttc gcaggtctga
      181 gccgcaggac tcgatgcgga cgatgatggg tttgttattc accatgacca ggtaggattg
      241 gtccggaaga agactgactt ccaacgcatc caattgcccg gcggtgaagt cccccctcga
      301 ggtatgggca tggaggatat cctggttcat gcaggacgcc tacgggccag tttggacata
      361 aaatacactt caaaaaaagt gaaaaacagg taggcgatca ggaagctgag gacatgctgg
      421 atccccagcg gggcgatcat tttatcgtaa acaagcacaa tacccagggt caggaccagc
      481 ttcaaaaaga tcatgcccat gaccacgccg gtaaaccggt ttttatcctg gtggcctgca
      541 gaatggaggc ccaggtggaa gatcagaatc gaaaacaaga agaaaaagat caggccgacc
      601 aggggaaacc agcccgattc aaagccggaa tagcgggtaa ggaagtaaag ggtcattccg
      661 gtaaggcccg tgatgaaagc cagttggata taaaactgcc ggatgctcat ttatttttta
      721 gacaggtcgc ggattagttt aacaaaatag gcagaaagga acaaaaccgc aaaagcaagg
      781 gaaaaatagg gttttgccgt attcagataa cggtccgcga aactgcccag ccaccagccg
      841 gccaccaggt acacggccat ctggaaagcc atacctgagt atttcagtat ttcaccggca
      901 ggtttcctgg gattttccgg ttgagtcaaa gttttcagac ttgatctacc tccgtgtaaa
      961 tcccgttaga ggaggaagtg accacctggt gctcttcatt tttcatcagg caggacacat
     1021 taaagaccgc gccggcatgg acgaccagtt tatcggtgtt gatatcccct ttgatattag
     1081 cggtctcttt tacctggagc gagtccctca ccgtcaggat accggtaaaa tggccttcga
     1141 tcaccgcgtt ttcacattcg atgtctccgt ccaccacccc gctggggcca atgatcactt
     1201 tgctgtgaca gatcagcgaa cccttgaggt tgccatcgat ccggatgtcg ttgtcacagg
     1261 tgattttccc ttcaatccgg gtgccgctca ccacgctgtt gagggctgaa gatccggcgt
     1321 ttgaagaaat gcttttgacc ggggtactgg tggttgattc tgtttttttt gagccgaaca
     1381 tttttttggt ttttatatcg tgagcaaata aaataaaagt caaagaaaaa aacaataaac
     1441 tccgtcttgc gacgaggtat tgtttttcac gggactacca aatttgtcgg tcgaaaaaaa
     1501 aatggagatt aaaaagtagg gcaatagacc cctctcctgg aggatccatc acagatattg
     1561 taaatcaggg acagttcaaa tgccccgttg ccattgcttg ccggcctcag gctggaaatg
     1621 ttcaagtcat aactcaggcc gatgccgtat tggttgaaat caaagcggga ggagacaatg
     1681 actgcatccg tggtaaatgt tttttcaaaa tggttggcca accgaaccca ggccccaaac
     1741 tggaaggatt gttcaaaatc cttggctttc tgggtgatga atttggcact ggtacccagg
     1801 ttgagttcga aagcttcacc ctgcctgaac atcaccaggc cggggaccag gctcaggttg
     1861 tttttcaggg ccatttcgcc tccggcatgt accgtgattt tactggtcag ggggaccagc
     1921 cggtcattgc tgaaggactg gttaggctgg ttgaggtggc tgtaggccgc ccccacataa
     1981 aaattgtttt tatccaggac actgaaccag agcaagccaa cattcaggtc ggcaaataca
     2041 aaattgtccc ggtccagggc ctctccgggc agggtggggt caaatcctcc ctggccatcg
     2101 tgctggctgc cgtactgggc ttttgtaaaa tcaatgcttc tttgggccag gcccacatca
     2161 aaaccggcgg ccaggtaatg cgagttggtc ctgctgccgg ccattctttt actgaaggaa
     2221 cctgccagct tcccctggat ggtcccgaaa ttaagctggc ctgccacatc gccccaaaac
     2281 ccgcctccaa tgccgaaata gtcgtacctg cccacttcaa tccgctgatc gtaggaagcg
     2341 gcgaaggtat tgtaggcatt ggaccggagc acactggacc attggttgcg gtaattcccg
     2401 ataaagcgtt tgttgcagtt catgaccccg gtcatggccg ggttgagatg gatcggggac
     2461 atgtaaaact gggaaaaatg aatatcctgt gctgtaccgt aaatacagga caacatcaag
     2521 accaatacgc aagctttttt catctatccc agactataaa cggtaaaaat aaaaattaat
     2581 tggctaagcg aaatgttaaa ataacgaggc tattatttac gcttgcccag ttgaatgccg
     2641 aggtaaaaat tgatgaaata agcggaattc ggagcttcaa tcataatggg aatcttgcgc
     2701 aggtaaagat ccatcatcag gcccgcttcc acggatttca catactcttc gacatctccg
     2761 aaggtgaaat taagggcggc tttgccctgg atgccgggaa tcatccggag ttgtgtgatc
     2821 cctttcagga aggaggcgga cccggcaatg ctgttcaggt cgagaaagtc ctcccggttt
     2881 tcctcactgt aacgttcggc ctggacaaaa taacggtccc cgttgtacct gcggatatcg
     2941 aggtaataag gctttgtaaa acccagggaa ggcccgcctt cataggccag ggctacggaa
     3001 attcctttcc tttttgcttt ttcggaaaaa tatcttttcc ccccgattcc gccccggacc
     3061 gccatgaatg aattctgttt accatagata aaactgttgg cgctggtgat gttgtgcagg
     3121 atcgactggg tgcgcaggct ttgccggtat tccttgggat gttttaaggt accgaaatcc
     3181 aggtggtaga acgtcgtgag atagtatttt ttaatggtgc ctttccggta acccagttta
     3241 aaaccgttgg tctgaaggtc ggcttgcagg gtatactcac tggtatagag gacgccgctg
     3301 tacccgtaat ccatctgcct gggctggatg gtcgtctgcg cccggaccat aaatgctgta
     3361 cccagggtaa aaaggcccag ccaaaacggt ttcatacaat cagagcgcat cggtcactaa
     3421 aattagtcga aatgtttttc atgaacaaat aatttaactt taggccctcc agaacaggga
     3481 ttttccgggc taaaatcggc cccaaacgag ttgatctatg caaacaaatg cacgcacgat
     3541 atggattgtc attttatccg cgctgggtct ttacctgctt tactactttt cagacctggt
     3601 ttcatttatt ctggcatcct gggtcttgtc catgctcgga cagccgatca tgaagctgtt
     3661 tcttgaaaaa ctcaaactga acaaattcag ttatggtact tccgtggcgg cggtgatgac
     3721 cattctgttg tacatcctgg ctatcctgct catcggcgtc ctttttatcc ccataatcat
     3781 tgaacaagcc tcgcacctcg ccaacatcga ttacgataaa atcggggctt ccctgcaacc
     3841 ccccatcacc tatatcaata atgaactcca gagcctgggc ttgatttctt ccgagcagtc
     3901 ggtcatggac cagatcttcg aagtggtgca aaaatggttc cggccggagc gggtttccaa
     3961 tatattcgga tccatcatcc agatcgggag caacctcacc ctggccgtgg gttccatcat
     4021 ctttatcacc tttttctttc tccaggagtc tggccttttc caggaatacc tggtggccat
     4081 cgttccgaaa cgccatgagg aaaaaatcag ccatatcctt tcggatgcca gccgcctgct
     4141 tcgcagatac ttcagcggca tcctgatcca gggatccatt ttttgcctga tcagttacat
     4201 cgccctcacc ctgttcgggg tccggaatgc cctgttgatc ggcttttttg gcggcctgat
     4261 gaacgtgatc ccctatgtag gcccgatcat cggcatgatt ttcgccgctt tcatcaccct
     4321 gaccggttat gtcggagccg atttcagcca ggtcatcctt cccctttgca tcaaggccat
     4381 cggttcggtc tttattgccc aggtgatcga caactggctg gtgcaacctt tccttttttc
     4441 aaaaaccgtg aaagcccatc ccctggaaat tttcctggtg atcctgatcg cggccaaaat
     4501 cgggggcatc accgggatga tattggccat tcctgcctat acggtgctgc gcgtggtggc
     4561 aaaggccttt ctcagtgaat ataaaatcgt gaaaaagatc accgaagata tggctgacca
     4621 gcccctggaa attccggaag aggcccccgc ggatgaggcc gcgaacgata aaccctgatg
     4681 ggctgcggag cattcaaccg ctttttctga tggattccag gtacagtttg acgctgttta
     4741 cggatactgt tttgtccaaa aaataggccg cagccgattc cttttcttcc gggctggccc
     4801 cggtttgggt caggatatta ttcaggtgca ttttcatatg gccctgctgg atgccggtgg
     4861 tcacgaggga acgcagggcg gcaaaattct ggatcaggcc ggtagcggct ataatcatca
     4921 tcaggtcccc ggcgccgggg ttttcaagca tttccaggga acgtttggca atgggatgga
     4981 gtgaggtgag tccgcccacc gtgcctacag ccaaaggcag ttccatccaa aatcggaaca
     5041 tcccctggtc gatggcacaa tggctcaaac tctggtattt tcctgtcctg gccgcgtagg
     5101 tgtggccgca ggcttcaatg gaacggaaat cattgccggt ggccaaagcc acggaatcga
     5161 tcccgttgaa aatgcctttg ttgtgggtac aggcgcgata aggatccacc tgggcgatac
     5221 gcaccgcatg ataaaaccgt tcggcaaaag tgagggcgtc catccttccg ggaaaatcac
     5281 cgaggtccgc aatggggcat tcgacccagg tccgcacgag gcaatcggga gtatagttgg
     5341 acaggatggc catgatcacg tcgatctccc tttcgtcgcc gtccagagcc ggatccgact
     5401 taaaaaattc ctgcaaggtc gaggagaatt gttcgagcac cgtattgatg aaattggcgc
     5461 ccatggaatc ccgggtgtca aactccgcga agagctggta gagcccgggt tccctccccg
     5521 tgaagctttt ccaggtcagg gatttgaggc ctcctcccct tttaagcatg gactccatca
     5581 agccgcggga ttcttccagc agtttgggtt tgagaagttc gaacaattca ccgaagcgtt
     5641 caggactgcc tgtccagtaa aaatggacat ggccgatttt caggttgttg atgatcacgg
     5701 cttggatgcc gccgcgttcc atccaatatt tggccgcact ggatgccgcc gcgaccacgc
     5761 tgctctcctc cgtgaccatg ggcacacagt aggtcttgcc gttcaggaca aaattgggta
     5821 cgacgcccag cggcagggga taattgctga tcgtattttc actgatcccg tccaggagtt
     5881 cctggtgctc ttggttgtcc agccaataac tcttcagttc atgcatgacg ttttcgggat
     5941 ccttaaaaaa attctcgacc agccatttga tcttgccgag tttggataat tttgagaaac
     6001 cggtaattgt ttttggttgc atcctgaggg tatcctgtta tcttacgcgt aagtatccat
     6061 aagccatctg cagcccgtct atctgtcccc gggcatgatc gtaaagggca tcataacttt
     6121 ttctggccgg ttgcaggaag cccgaagcct gaccgtaaac ggacttgaac cgcagctttt
     6181 gcatataata ataaccgtcc aggaaatctt tgatcccgcc ggagatgatc acgcttccac
     6241 attttacctg gtcgccgagc cgggatacga tttcgttcac aaaacgggtc atttcgccgg
     6301 cggtatgacc gacccggctg aggttgtcgt attccccggt atttcccgcc gggcgtcttt
     6361 tcatttccag ggttgaaaaa ttggtccccc ccagggcggc aaattctatg gctgcgaggg
     6421 gcagcttcaa cagggcttcc aggctttccg gccccatgcc ttgcccgact tctttaacga
     6481 tcaggggcgc tttgatgttt tggagtgtgc tcacgatggt ttgaaggggg ggatgcagga
     6541 tgggatcgcc ttccggctgc aaccattcct ggagcgggtt tacatgaatg atgaggccat
     6601 ccgcttccag tcgttcgatc agccgctcta ttttatccca ggcttcctgc aacaggatct
     6661 gttccacctg ggcgatgccc aggttggcat aaaggggctg atcgcccata aattttctga
     6721 ccttaaaatc tgaaagatac tggtcggaat cgagcaggat cctgcaggat ccaaggccca
     6781 tccccaggcc gaaggcttta cagatcctgg ccaggcgttc gttgatgacc accgcttccg
     6841 cggtgccgcc cgtcatggat gaaatccaga ttggattttt caggaccttt ccgtcccagg
     6901 gggtttcggg gatgtttgaa tggctcgggt gcccgctgag cagcggttca taataaaacc
     6961 gctcatcggc ctgcagggca ttcatcctgg attgcaaggc aaggtcgaga tgatctttct
     7021 tgcgctgatc ggtgagctgc gggctctgct tttcttccat gtgttgataa aattccgaaa
     7081 taaattccaa atcttgtcaa tactgaaaca agccggccgc aagttggttt aaccgttcat
     7141 cacatgtttg aacgcggtga cggttaagtc tttatccact ttcttcaggt attggtctat
     7201 gttcaatttt gaatctttga cgaattcctg ctgtaggagg gtattttctt tgtaaaattt
     7261 attgagttta cccagggcaa ttttttcgag catttcctcc gctttgccct cctggcgggc
     7321 gacttccttg ccgatctcga tttctttttc gatcacggat ttgtcgactc catcaggagt
     7381 gacggctatg ggtttcatgg ctgcgatctg catggccaca tccttgccgg cctccaggta
     7441 ttggtctcct gctttgttga ggcccacgat gaccccggcc cggttgccca tatggatata
     7501 aggaacgacc aggggcgcct ccagtctttc ataactttcc agcccgattt tttccccgat
     7561 ggcggctacc atatcggtga ctttatcgcc gatgctgacc ccgttgagtt tgagggaaag
     7621 cagttcttcc aggctgggcg gaaactgttc gagggccacc tgggcgattt gcctggccgt
     7681 atttataaaa tcttcgtttt tggaaacaaa atcggtttcg ctgctcagcc ggatgaccac
     7741 gcctttggag cggttgggag aaaccagggc aatgaccgcg ccttctttgg cttcccggtc
     7801 ggcgcgctta acggacaatt tctggccctt ttttctaagg atttcaatgg ctttttcaaa
     7861 atcgccgccg gcttcactca gggcggattt acaatccatc atgcctgcgc cggtcatttc
     7921 acgtaatttt tttacgtctg ctgcggatac ttcactcatg aatataaaga gacttaagaa
     7981 tgataaaaaa aaataaaatg gattctgatt ccccgaagga atcaggcatt ggcggccgcg
     8041 ccggtttctt ccagttcggc tttcatctga cgcctctctt cgaggccttc cttgatggct
     8101 tccaccagca cggaggtgat gatgcttatg gatttggagg catcgtcatt ggccgggatc
     8161 gcaaaatcca ccaggttcgg gtcgcaattg gtatcgacca tggcaaaagt tttgatgccg
     8221 agccgggtag cctcctgcaa agcgatgtgt tcgtgcccga tatctaccag gaaaagggcg
     8281 tcggggaggc gattgagttg ggatatacct cccagttcct tgtccagttt ggccctttcc
     8341 cggtccaggg tcagcctttc ctttttggtg atgctgctga gttcattgcc cgctgcaagc
     8401 atcctttcga tgttgtgcat ttttttgatg gaacggcgga tggtcgcaaa attggtcatc
     8461 atcccgccca gccagcgttc ggtgacaaag ggcatgttca cggtcctggc ggcattggca
     8521 atgatttcac gggcctgctt tttggtggct acgaacatga ttttgcgtcc ggacttcacg
     8581 atgttccgca tggctgaagc ggctttctcc aggttttcag ccgtccggtt gacatcgatg
     8641 atatggatcc ctttatgctc tgcaaagatg tattggcgca tcttgggatt ccatttcctg
     8701 cgaagatgtc cgaaatgcac acccgcatct aacatttcct gataggttgg aattttcatc
     8761 agaatagact ttaagtttga attctttaaa ttaacgttta gaaaactgga agctcttgcg
     8821 ggctttgggt ttgccgtatt ttttcctttc cactacccgg gcatcgcggg tgaggagttt
     8881 caggtccttg agcggctttt tgtgctcgcc gttgattttc accaggcacc tggaaatacc
     8941 catgcggatg gcttcggcct ggcctttaaa acccccgcct gaaaccagca ctttcatatc
     9001 gaagccattt tgttccactt tagccgcctg gaagggttcc aggagtttgg cctgcatgtt
     9061 gcgcaacggg aaataatcgg ccagttcgcg gccgtttacc ctgatctggc cggtgccctt
     9121 ggacaggtaa acccttgcta ctgcagcttt gcgtcttccg acaccattta taatttccat
     9181 atatgattag tttaagtgta tgagatcaaa gggtaagcgt ttccggtttc tgggcctgat
     9241 ggggatgttc actgccggaa tacaaaaaca atttcttgaa catttgcctg ccgaggctgt
     9301 ttttcggcag catgcctttg acggcgtgtt caaggatata gtgcggcttc ctggcataaa
     9361 gctctttcgc cgttttggtc ttttgaccgc cgggatagcc ggaatagctc tgatattcct
     9421 tggtcagcat tttttttccg gtcatcctca ccttatcggc attggtgacg atcacaaaat
     9481 cccccgtatc cacatgcggg gtataggccg gattggtttt acctctcagg atggaggcga
     9541 tcttggtgga taatcttccg agcgtaagcc cgtccgcatc cacgataaac catttccgtt
     9601 tgcccgtaaa atcttccttt ttggccgaaa tggtcttaaa agataatgtt ttcactggtc
     9661 actgaatttt aattctttta cctaaagagc cgcaaaatta caggaatgcg cgcataccca
     9721 caaattattt ttgaaaaaat ctttttataa cgccttgaaa aacaagtgtt tttttgctca
     9781 accgattata cggagccttt ttctgaactc agacctcaag ccattcctct ggcaatcagc
     9841 taagaatcag gacctttcca cggttttgaa gccgggagcc ttataggtgg aaggcttacc
     9901 atccggtgcc aaagggactt gcgtcaagcc ggttcatcgg atgggtggtt gatcccgatg
     9961 cgggaagaag gccggcggga cttaaaaaat ccttgctgaa tcgaatcatc caccaccggt
    10021 tgaaatcctg ttaattccaa ttaaaaaaat attaattgag catagctttc cggagagggt
    10081 atcagttttt ttctattttt aacgctttga aaaaccataa tatcttatac taaatgtatt
    10141 tcaactcaga tcttcaggct gaaaacacct atgatgcgat cgtggtcggt tccggtattt
    10201 ccggaggctg ggctgccaag gaattgaccg aaaaagggct gaaagtgctg gtcctcgaga
    10261 ggggtcgcat ggtcaagcac ggggaatatc caactgcagc gcttgcaccc tggcaactgc
    10321 ccaatggcaa ccgggtgacc cgtgaagagg ctaaagatta cgaaaaacaa tcccgcaccg
    10381 gatataccat ctcccaggcg accaagcact ggtttgtcaa ggataccgaa catccctatt
    10441 ccgaaaccaa acggtttgac tggatccggg ggtaccatgt cgggggaagg tccctgatgt
    10501 ggggcaggca ttcataccgc cacagcgaca tcgattttac cgcgaatgcc aaagacgggc
    10561 atggcaccga ctggccgatc cgttacaagg acattgcccc ctggtacgat tatgtggaat
    10621 ccttcgccgg aatcagcggg atgaaagaag gcctggaaca attgcccgac ggtaaatttt
    10681 tacctgccat agaactaaac tgtgtggaga aaaaagtcag ggaatccctc atggtcaaat
    10741 acaaaggcgc ccgggtcctg agcagcggcc gggtcgccaa tctcacccag gagcacaacg
    10801 gccgttccaa atgccagttt agaaacctgt gcagccgggg atgtccatac ggagcctatt
    10861 tcagcagcca gtcttcgacc ctgccggctg cagagaaaac gggcaggctc accatccggc
    10921 caaactcgat cgtaaattca gtgatttata atcccgagac caaaaaggca accggggtcc
    10981 gggtcatcga tgccgaatcc aaagaaagca tggaattcca ggccaaggtt attttcctct
    11041 gcgcctccac catcaattcc acctggatcc ttatgaactc cgccaccgaa atctaccccg
    11101 atggactggg atcctccagc ggggaactcg gccacaacct gatggaccat catttcaggg
    11161 tcggagccag cggggagatg ccgggattcg aggatatgta ttatttcgga agaaaacccg
    11221 gaggcatcta cctgccccgg ttccggaacg tcggacagga caaacaaaag gacttccttc
    11281 gcggctatgg ttaccaggga ggagcttccc gcaccgggtg gcaacgttcg gtcaaagaac
    11341 tcagtttcgg cgaggattta aaagaaacgc ttggcatgcc gggccagtgg accatgggat
    11401 tgggcggctt cggcgaatgc cttccttacc acgaaaacaa gatgacgctt aacaaggacc
    11461 tcctggacaa atggggccag cccacggtga ccttcgattg tgagtttaag gaaaacgaaa
    11521 tgaatatgcg caaggatatg ggcagttccg cggctgaaat gctcgaagcg gccggtgcaa
    11581 aaaacatccg ggtaaacatg cccaacgaca atgcacccgg cctcggcatc catgaaatgg
    11641 gtacggcaag gatgggcacc agtcctaaaa acagtgtact gaatgcccac aaccaggtct
    11701 gggactgcct gaatatctat gtgaccgacg gagcctgcat gacctccgcc tcctgcgtga
    11761 atccttccct gacctatatg gccctcaccg cgcgtgccgc cgaccacgcc gtgaatgaac
    11821 tgaagaaaat gaatatgtaa atcctttttt aaaatttgaa attatacaag atcatggata
    11881 gaagagaagc gttgagaatc agtgccctgc tcatgggcgg ttctttatcc gctgccacca
    11941 ttgccggggt aatgaatggc tgcaaagcca gtaccgtccc ggactggacc cctgcctttg
    12001 tttccaaaga actcgccaac agcctggctg aactttgcga aaccatcatt cctgccggaa
    12061 aaacaccggg cgcgaaagat gccaaagtag aacggttcat cgacgtgatg atgaaggagg
    12121 tcttcaaacc ggaagaccag gaaactttta ctaagggcct gaaacaactc gattcagacg
    12181 cccggtccaa atatggcaaa gccttcgaca aaataaaacc tgaagaaagg gtagaaatgg
    12241 tcaaagccat ggatgatgaa ctgagagagc gtaaagagca acccgcttcc aaaccgttct
    12301 atgacctggt caaggagttg accgtcaccg gttattgcac ttccgaacct ggcgcaaccc
    12361 agcacctaaa atatgttgcc gtgcccggag attacaagcc ctgcattccg ctgtcggaag
    12421 tcggaggcac ctgggcgatt taagtgggtg gtgcttggtg gttagtggat ggtggatcgt
    12481 caaacttgcc aacttgccaa cttgccaact tactaacttg ctaacttgct aactttaata
    12541 tactttctgt aaaacaaaaa ggcaagcccc aatgccagga agggagccaa accaagtata
    12601 tatccaaggc tgatcagcag atttgggaat agtggagcct gtgagccctg gctttgatat
    12661 ccggcccaga aagcgggaaa agcctggcca aaacgggcat tgcggataaa attcaattgc
    12721 atatttcgta tggcttcgtc ctttggctgt cccgcacgca gaaaacggta cacccggacg
    12781 acctgctcct tggaaagatc gtccctgatg ggccagaggg tgctgatcac actacggctt
    12841 ccggcctgga aaaaacttcg ggcaagggac atcatccctt ctccggatac gtatttgccg
    12901 gtgccggatt cgcaggcgct taaaaatacc aggtcctgcg gcagatgcat cacaccgagt
    12961 tcacccgcaa gcagggtatc ctcctggagc cggataaaac tttctccaaa ttggtcctcg
    13021 ttggaatagg cgtggctggc aatgtgtaaa atgcggccct gtcccgcaaa cttcataaaa
    13081 ttggcgcgat tggcctccgg cccgaaaaaa tcggaggccc tccccaagag gctggagatt
    13141 tccttcgttt cctcccggtt gtatgtcagg tcatgaaaga ccggcgcaaa ggaaaccagg
    13201 gactttttac cggagccttg ttttcctaca tccgtcttcc gggtctggtc caaccacaag
    13261 gccaaagagt attgtgaact gatcacatgt tcgcgaacca ggtagggcca ttttttataa
    13321 tcggaatccc cgacttcctc tgtcaaaagg atgccaaaag gcagccttgc caattcaaga
    13381 tcaggcatga ggaccagcct gcgcttcagg tatggcttta tgggctgcaa gagcccccgg
    13441 aataatttta aagcagattg ccggtataag gaatccctaa gcctggtata cctggattca
    13501 tgggccatgg atatgctcag ccgaagatca ttgatatcct gcccgatggt gtccgggtgc
    13561 ccgggcaagc ggtataatcc aatatggtcc gcattgatca aaagtgcgta gatcctggat
    13621 ttgcccttaa aatactccaa cagggtggtc tcctggtcca gaagggattg caggtgggag
    13681 aggctgcccc acctcttttc aggctgccgg gtatacatcc gcttgaggtc ccgaacctgc
    13741 atctgtaaac tattatgccg gcccaggagg gaatcataag ccgggccgcc tagctccagt
    13801 tgcgttttaa cctcccggag ttccgaagac aaccttgtga atttgatcag gtcctggtga
    13861 ctgatgcttt tcctaagccc gtattcggaa tgcaggttga atgatttacc cgcttcaaaa
    13921 caggtcagga cggattcata gtccgggaca tagggggttg aaggatctgt atacgtttcc
    13981 agggccaggt caaggatggg catcagtctc cgcacaaaac tcagccggtc ctcatccgag
    14041 atcaggtcgt tccggatcga ttgcagcccg gtaataaaat gcttaagtaa ttttgttttt
    14101 ttttcaagaa aaaaggcatc cctgcattgc acatagccct gctcatgata ctgaatgccc
    14161 ttcatgacgg tttccagaag ctgggtcctg agccccaggt cgccccatag ggaatcggat
    14221 gatctatgga tactcaggtg gtacaaggcg gaatccatgg catccgacat ggccaggaaa
    14281 tccccggaag taaccgccaa tccggcttta gccctgaaat aagcagccag gtccaccgga
    14341 tgcctgatct gtttttccaa ccgggccagg atgggtatac aactgtccct ccggttaaat
    14401 tttaagaaca ggtcgagctt tgcgaattgt acggtcagat cttccgacac caatgcattc
    14461 aaatccatcc gggagacgag tttcaggccc tcttcccaac ccagggcgtc gctttgcaat
    14521 tgagcaaata aataatcttt ttcccgggtc agcgagtagg cgctggattt ttgaatattc
    14581 tggtattgca aaagataggt atcatacaac tgccggtcac cggacttttg agccaggtcc
    14641 aacagttttt ccagggctat ttcttccgat tcctgggtta gcggcccggt gcgggaaaaa
    14701 acctggtccg ccttcctggc ttgtttcagc gcttcataaa aataattgcg cgcttccaca
    14761 tattcatggg cgtccatggc catcttgccc agccgccggt agcattgaaa caaacggtgg
    14821 gccagggact cgctgctgcc ccgtatgggg tcctcgccgt aaaactgagg atgatccagt
    14881 aaaaatttga tggctgcatt gagttgcgat accgcctggt cgaccttttt tttatcttcc
    14941 agaaaatcag cataatagat gtaataggca aattgccgga ccgcggtctg gaaggaatgg
    15001 gcttcgccgc tccgtcgatc caaactttcc cggattgcat tcaattggtc ctccagtaag
    15061 gaatccaggg atagttcccg gtatgcctcg gtaaggtata attcagccag gttgtggaac
    15121 cggctcccga tgggacctgc actttgtgat gcctgcttca ataagtcctg gcaaagggtg
    15181 atggagatcg ggtaatttcc gttttcctgg gcttcattga tcagtttcaa taaatgatcc
    15241 aacctgatag aatccggctg ggatgcgcaa accggaaggg aatggaaaaa taccgataag
    15301 aggaaaaggc agttcagttg acaaaccttg gtacggtgta agaaagccca agccataata
    15361 tttgtctgaa aaaaccttga acctgtgatt taaatacggg tgagaaaagg taattcctcc
    15421 taagaaacta caaaccaccc cttccaaata taaagactta acggccttaa ggtgaacttt
    15481 aatttcttct gacttttaat cggatttcca gcgggcttga tcccggaccg gaaaaaatac
    15541 ggatgatgac gttggtgccc cggtaggctt ttacccttga ttccgactcc ggctttggac
    15601 taaggtaata aagcgtatcc gtggcttctg ccccggggct tagagagacc ggggtggtcc
    15661 gcttgtcata atcaaccagc accttgccgg tttcatccag aatttcagca tcgaaattgg
    15721 aaaagtccac catgtcattc aacaaggagt tctggtaaat ttccgcggcc atgacataac
    15781 agggcgggga ttgtctgcaa tctgacgatt ttgacatgga atattattta tgaggattaa
    15841 acccgaaagt ccttaggaaa aaagccatga ccctgccaaa ccagccggtg ttgtagatct
    15901 gcttgacggt cccgaaactg gcttcaatcg attcttcggt gaataaggcc ggccgcttga
    15961 gctcggcctg ccttaacgcc tcctgcataa gtttactgta atggagggct atacccggct
    16021 cgtccttaag tttgctgtct tccatgagct gtccgtaagc cctgaatttc aattcatcta
    16081 ccagaaatac ttttgaataa tccaatgaca taagctggtt tgttaaatat tttaaaatgt
    16141 atttcttttt ggaaaggtta cctgaatttc catttttttt attaagaaat gaaaaaaata
    16201 aaaaagacat cacaacaaat tgatttataa ttatttatat ttgaatgatt aattattacc
    16261 ctttaaaaga cggacattct aaaaacaaaa gacatttatg gcaaaggaga ctgatgaaga
    16321 attggtgaac atgataaaat caggtgggcc cgccagggaa aaggcgcttc atcggattta
    16381 caccgattcg aatatcaggg agaaaatcat cgccatgatc accaataaca atggaaatca
    16441 acaggatgga gaggatatgg tccaggaggc catcatcgta atggataaaa atatccggga
    16501 aggccggtac cgtacggaag ggaatctgca atcctacctg tttgccatcg gtaaatatct
    16561 gtggatgaat cagctccgca agaaaaaacc catgctgaag ggcagtttct ccgatttaga
    16621 aatgaccggt gatcctgacg agccggacca tttcctgctg gatgaagaac gcaagatgaa
    16681 gttgcaggct atcctgtcca gactgggcaa ccgttgccag gaaattctga gcctctggca
    16741 gctttctttc agtatggaag aaattgcagc ccagttgaaa ctcaacgatg catccactgc
    16801 ccgcaaggca aaatacgatt gccagcaaca actgatcaaa ctaatacaga aatcctccct
    16861 caccaaatcc gaccttacat gaatcaaatc atacccgacg aggccttact cgaccagttt
    16921 gaagcctatc attccgggcg catgaacacg gaagaccaaa accggtttgc caaccggctg
    16981 gccacagagc ccggattcaa cgaatcttac catggctttc tgaccagccg ggaagccatc
    17041 gaaatcaaga tctccgatga ccttaaagcc aaaatgaaaa aatggcaaaa acaaagtttg
    17101 cccggagggc tttcagaggc cgggcgcatt cgccgcctgc cctggtccgc ctgggcggcg
    17161 gccgctgccc tcttattcct cctgactttt ggttttatac aataccggtc ggtgcagtcc
    17221 tatcccctgg atgccatcga cgaatatcag ccggtccagg ccctcacgct gaggaacgga
    17281 gaccaggatt ccggcctgaa cggaatcatc cgcgattaca ccagtgaggc cgccacttta
    17341 gatcagacca tttccgccct tttaaaaatc aggccggaaa ccccggggaa tgaatatcaa
    17401 aaggcccaaa gctacctggc aaagctctat ttacggcagg gactttgcgc ggatgccgct
    17461 gcagcctatc tgcggagcgg tgaacttgaa ggcgcccggg acgaagccga aatcggcgct
    17521 gtgctttgcc ttatccggga aggtcatacc cgggaagaga tcaacggagc cttgaaaccc
    17581 atccttgacc atccgaacca ttcttatgcc ctggccgccc gggaaataga tcaaaaaata
    17641 aatggcttct ggtggagatt atttaaataa aagttcagcc tatcacaccc atcacagtcg
    17701 tccgggaatt cttcgaccat atcggggatt tgttttttcc ccggatatgc ctggcttgtg
    17761 ataaaaacct tcaggccagg gggcacctgt tctgcattca ttgttcctat accatagcca
    17821 ttaccgacca atggaaaaaa ccggaaaatg agttcacgga aaaatttgcc ggacggctga
    17881 aactggagca gggtgcggcg ctctaccgtt ttgtacccgg cggacagctg cagcaggtga
    17941 ttcacaagct caaataccaa aacaggccgg atatcggcat ccacctgggc cgcacagcag
    18001 gccaccagat ccaccggtac tggccgcttc cggatcttat cgtaccggta ccgctccacc
    18061 cgaaaaaata ctggcaaaga ggttataacc aggctgaaca gatagccagg ggcattgggg
    18121 aaatcaccgg tatcccagtc ctgcccaatg ccctcgaaaa gaccgggtac acccggtcac
    18181 agacccgtct ccaccggctc gaacgcatgc aaaacatgga aaagtctttc gtgcagcacc
    18241 cgtccgtaaa aatgattggc aaacatgtgc tgcttgtcga cgacgtgctc acgacggggg
    18301 ccaccctgga agcctgcgcc ctggaaatcc ttcggaaccc gggaagcagg gtaagcatgt
    18361 gcacccttgc catgggaagg agaatttaac ggacacttcc tttccccaat catagtcata
    18421 ccccctatat ttaggccatg acagcaactg aactcgtaaa aaaagaatgt ctccggcgaa
    18481 taaatgaaag ccatgaaaaa ataatatcct cactggccgg gtttacccag gaagagatct
    18541 ggtggagacc caatcccgcc agcaacagta tgggcaatat catcctgcac ctctgcggca
    18601 acctgactca gtatgtcctc agcagccttg gcggatggcc ggatcagcgc cagcgctcca
    18661 tggaatttga agagcagggt cctgtcccgg taaaggaatt gtttgacaaa ctggaagagg
    18721 tcatgaggaa tgtcaatgag accatccatg cctgcaatga ctatgaactg atgcgggtac
    18781 gccccgtcca atgttttgaa gaatcaggta tcggcattct catccatgtg acggaacatc
    18841 tttcctacca caccggccaa atcgtctatt tcgtcaagtg gaaaaccggg aagtccatgc
    18901 atttttatga cggagtggac ctggaagcca ggggtgaagg gtgattggtg aagggtgatt
    18961 ggtgggtggt gattggcgct ccgaacctcc aacctctaac ctccaacctc ttacctctaa
    19021 cctctaacct taatatttct aaacgcaatc tcccctccat gattctgcag gccgatgcgc
    19081 ccggttttat tcaggctcca gcctttaaac ggtttgaatt ttgatttggc caccagggcg
    19141 gtgtattctt tggacccgat ttcgtattcc acacatttaa ctccgttgag ccaatgctcg
    19201 acacggttgc cttttttaat cactccggct gtattccact gaccggcagc attgaaggtt
    19261 gcaccctggc aggacatgag gtcgtaattg tcggcggtca ggtgattggt tgctgcttcc
    19321 atttcatgcg tgttcacata gttgttgtcc acaagctgga actccggcgc agtctgccag
    19381 attgccagtt tgggattttc attgaccagg taaaacaggc cgctgtttgc agagtcggcg
    19441 agtttgaagt caaacttcag ttcaaaatcc ccgaaggtat cccgggttac caggtcgccg
    19501 cctaccatag ctccatgtgg gccttccggc ttaagtacga tctggccgtc catcactttc
    19561 caggtatggg ggatgctgtc tccgttgtac accctccaag cgtcaaggct tttcccgtca
    19621 aaaagcagtt tccagccgga agcagcttcg gcatcggtga gcatattgtt ggagtccacc
    19681 accatggcgg tggtatccgc cgctggggcg gttccggtgt cagacatctc cttgttcttg
    19741 cagaaagtaa gggccagggg taatataaag aagaacagtt ttttcatgat acgtttttta
    19801 attggcacaa agttaaaaag ggcttcaggc attgtgggcc aggatctccg ttaacgatct
    19861 tcaaatcccc agtattttcc gtcccatttc cacattgcaa acgtcagggg tttctaatcc
    19921 tgcatacgcc cgggccgaag aatatttcca ctccaaggtc tcccggacca gaccagaaac
    19981 cattggattg tcatgtatat aatgaaaaca tgcgtaggca taatcatttc ctgtggtaaa
    20041 ccggaagtcg gttttgccat tggatttttt taaggtgaca aactgatcga tccatccatt
    20101 ttttgatttg cactttgccc tgaaaagact tccggtccaa ccgttttctt tgtttatggc
    20161 gcgggtatac gatcggagca aaatgccaat ggcttcattg attgaaattt ttgaatggtc
    20221 attggatttt tcccatcggt cagtataaat tcctgaaaaa acatttccaa attttatctc
    20281 tcttctttgt ctttcaattg agttcacata cagcctgagc gatttccttt ctattaagtc
    20341 gcgatttata taaatcagcc agtgaaagtg atttggcatc aggcaatagg atataatgtc
    20401 agcaaatggc gtgatgtaca ttttcatttt ccataaaaag tattgatagt tttcgtcgga
    20461 aaaaaatatc ggtcgacgat tattcccttg attataaaca tgatagagct ggttcgcata
    20521 taagttcatt tttaaaaata ggtggaaatg ctcaaaaatg aaggggtgac ttaagcgaag
    20581 gggtgactta agtcatcccc tcacttaggg cattgtctat tttttcatct atatttgtcg
    20641 aattatccga catatcatgt atctcaacgc ccacagctat ttcagcctcc ggtacggcac
    20701 cctctcccct tcggagctgg ccaccggagc ggccgccctg ggccatacct ggatggcgct
    20761 cacggatata aacaacacct cctgcagctt cgaattcctc caagcttgcc aaaaagcggg
    20821 gctccggggc ctcctcggca tcgaattccg ggacccggaa caccgttatc tctatacggg
    20881 gatcgcccaa aatgccgccg gttggcatga actctgcgcc ttgctgaccc ggagttcgct
    20941 cagcggggag cccctgcccg ccctggcccc tcccctggaa cactgttttg tgatctaccg
    21001 ctccctgccc aagccgctcg ccgatttcgc cccccacgaa tattacgggg tctcccccgc
    21061 cgaagtgggc ggcctgtatt catcccccct ccggaaggac cccgggcggc tcctggcctt
    21121 tgcctctgtc agccatcgcg accgggaagg ccaccgcata cataaactgc tgcgctgcat
    21181 cgaccgcaat atcgtgcaca cccgactgga agcggccgat acggccccgg tcacggacag
    21241 cctggtgagc cgggactcgc tgctgcaggc ttatgcccaa tatcctgaac tgatccggag
    21301 gaccgaacaa gtcgccgacc aatgttcgat cagcctcgag tccggcacgg gggtcaaccg
    21361 ccgtaccttc accggcacag cgggcggaga ctatgaactc ctcaccaaac tggcccggga
    21421 aggttgccgc cggcggtacg gaaccacaca caaaagggct gcagaacgta ccgaaaaaga
    21481 attgcaggtg atcttcaaac tgggcttcgc cgcctatttc ctcatcacct gggacgtcgt
    21541 gcgatatgcc cggagcgcgg gctaccacca tgtgggccgg ggcagcgggg ccaacagcat
    21601 cgtggcctac aatctcggca tcaccgacgt ggatcccctc gaactcgacc tttatttcga
    21661 gcgcttcatc aacccgcacc ggtcctcgcc tcccgacttt gacatcgact tttcatggag
    21721 cgaacgcgac gatgtcaccg actatatttt caaacgttat ggacatgaat acaccgccct
    21781 gctggccact tacaatacct tcaagggccg gtccatcatc cgcgaactag gtaaggtatt
    21841 cggcctgcct aaaaacgata tcgacctcat cgtcgacgag cccctggcct ccgacaaaca
    21901 ccatccctgg gcaaaacata ttttccgttt cggcaaaagc atcgaaaact ttcccaacta
    21961 cctctccatc cacgcggggg gcatcctcat cagcgagcgg ccgctcaacg agcataccgc
    22021 cctgcagatg atgcccaagg gttttgccat cacccatttt gacatgtacg gcgccgaaga
    22081 cctcggtttc cacaaatacg atgtcctcag ccagcgcggc ctgggccata tcaaggacgc
    22141 ggtggacctc atcaaacgca ataaaagaca gtccgtggat gtacacgaca tcgcccgcct
    22201 caaagaagac ccgaaggtga gggcccagct ccgttcgggc cattgcatcg gttgttttta
    22261 catcgaatcg ccggccatgc gcgggctgct gcacaaactg cgctgcgacg actacgtaca
    22321 cctggtggcg gcgagctcca tcatccgtcc gggggtggcc cagagcggca tgatgcggga
    22381 atacatcgaa cggtttcacc acccggaaaa aatccagtac ctccacccgg tctttgaaaa
    22441 aaacctcaaa gaaacctatg gcgtcatggt ctaccaggaa gacgtgatga agatcgtgca
    22501 ccattttgcc ggcctcgacc tcgatgagag cgacgtgctg cgccgcatca tgaccggcaa
    22561 aaaaaaatca tccgacacct tcctgcggct gagggataaa tatttcgcca actgcaaggc
    22621 gcgcggttat tccgaggccc tcacccagga agtctggagg cagatcgaaa gtttcagcgg
    22681 gtattcgttc tgcaaggcgc attcggccag cttcgccgtg gagtctttcc agagcctctt
    22741 tctcaaaacc tattatcccc gcgaattcat ggtggccgtg atcaacaatt tcggcggctt
    22801 ttacaatacc gagctctatg tacacgaggc gcgcatgtgc ggggccatca tccatgcccc
    22861 ctgcgtcaac cgcagcacct acctcaccac cctctacggc gacgacttgt ttatcggttt
    22921 tgtccacatc cagaacctcg aacagaatgt ggccgtgcgc atcgtcgaag aacggctggc
    22981 caacggccct taccgcagcc tcgaggattt tgtacagcgc atcgacgtca gcaaggaaca
    23041 gcttgagatc ctcatccgca tcaatgcttt ccgcttcacc cgcatgaaca aatacgaact
    23101 gatgtgggaa aaaaacatgg tgcacaaccc ccaggtcaaa tacgcccaga acggcaattt
    23161 tttccccgat gccgccgatc aatactcgct gcccgaactg gacgaaggcc gtttcgaaca
    23221 ggcttttgac gaaatcgaac tgctgggttt cccgctttgt tcccccttcg acctgctggc
    23281 agaacccttt aaggaagaca tcctggccag ggatatgccc gcccaccacc gccggacggt
    23341 caccatgctc gggtactatg tcacccgcaa atgggtgagc accaaaaaca acaaactcat
    23401 gagtttcggc acctggatcg accgtgaagg ccgctttttc gacaccaccc acttcccgcc
    23461 ttccctggaa gcctaccctt ttaaaggcaa gggcgtctac cgcatccggg gcaagatcgt
    23521 ggacgatttt gattttaaaa gcctggaggt gatggacatg gaaaggttgc cgtatcggaa
    23581 ggacgagagg tattgaaccc aaaccatgca atagagtttc tattacaaac cggaatgcct
    23641 gcaaatactg gcacttcccg gccgaagagg tcgatttatt attaaggtcg gcctcccatt
    23701 tttatcctca ccataggagt gcctatgttt gcggtaaaaa tagtccggga gcgccagtct
    23761 ttattggcct tccggttttc attacgaaac tctattgcat gatttgggtt gaagaatgaa
    23821 aaaataaagc cgaattattg agctctgcct gatggagtgc gcaaatcagg attccaggtt
    23881 actttaaacc aaaggggttc accattgcga taaaattaaa agccttagca ttgtgaatcc
    23941 atttatttaa attaatatag atcgacaatt atacaggagt gaaaaaaaaa ctactcatca
    24001 attggctcta ttaccatccg gtcgggcatg tcatcgagcc gctgaaactg gcaaagggat
    24061 atcaattggc aaacaaaaat ttagaagtat acctgataat caatgccgat tccccccggg
    24121 agcttcctga cgcctgtcca tggattaaaa aaacttatac cgtctcactg gatgaagttc
    24181 aacggtgtgg ggcgggtgcc cttcagaaaa tcccaaaaaa atgggactat gtcattaccg
    24241 ataaccgggc aaggtggttg gtatccggga gagataatga aaacctgatt catgcccaag
    24301 acttattgaa aaaattattg gtggcaaagg tcaaaaaggg atatgtagaa caatccggaa
    24361 tacagaaaga gagcatactg ccgattgtgg gcaatcctaa aattaaactt cctgttccgg
    24421 ataaagccag ggattttgca aagaaattta aacacagagg tcctaaaata gccattctgt
    24481 taggcggttc ctcaggtaag aatcgaagcc cgggcctgaa atcatggtta aggatatgcc
    24541 aggcgctcgt tgcggccatt ccggatctga aaatttattt taccggggta tcccggagta
    24601 taaatggaag aactgcgacc agagatttta accgggaaga tgtggatgat ctcatcaaca
    24661 ggttgcccgg tgctgaatat gcttacgatt tagggctgtg gaatcaaata gccctgatcg
    24721 aaacctgcga catttttctt tctccacata caggatttgc ctttattgcg cccctggtca
    24781 atacaccctg gctggaaata gccacctgcc cctggccggc ttatatcttc aatgaccttc
    24841 ccttctattc cgttttgccg gactgcggat cataccccgg ttggggtaat ccgcttaaag
    24901 gttgtgggaa attgttacag gaaaataaaa aagcacgctg cgtttccgat cggtcattaa
    24961 aaaagaaaat accagaaatc gtgagaggag ccagatacct gttggataaa aaatttacct
    25021 acaaaaaagc catcagcctt cacctgaaaa agatccgtaa aaactatccg atacagcatt
    25081 tctttttctt agggggcatt gaaggcttaa cccacactcc tggtgaacca atacctaaaa
    25141 accagtctta ggggtcattc ttcctcaatt aactcaatcc ctgccacttc ggatacccgt
    25201 ttatgtttta ccgaaaccgg aatcagtttg atgtggtgct ttcccccggg tatccggata
    25261 gcccctaatc gcatatttgc ccattctttt tcataggctt ccttcctttt cacccggtcc
    25321 gggctgggaa gaaaaggggg atcaaaagaa tcggtgaccg tagcctgaat gctttgctct
    25381 ccgagtcgga ccgaaatgac ggatcccaaa tcggttgggg ggcaggtata tttcagcagt
    25441 acttcaaaga cacgcatggt atccgattct accagccagc gaatggtatc tgcagtggaa
    25501 gaccagttga ccagccaatc atgcgcccag ccgtggcctt cggcaaatcg gagattaccg
    25561 gtgtattccg cttcataagc gggcaggagg atatgactgg tattcagagc gatggctttc
    25621 tccggcttgt agccttgccg gaccgaggca aaccactggt catactgatt tttcaggttc
    25681 cctgccaact cggtttgttc cggtgccagg ttggtggttt gaccgggatc cttgaccaga
    25741 tcgtataaat aatgggcagt atcccggacc accaggcgat gggtagcagt ccggaccgca
    25801 cccggatatg aaatgaggtt tttattcaag aaagcgacat gggtaaacag ggtccgggaa
    25861 ggaaattccg catggtggcc aaggatctgg gtcgaaaagt ccattccatc tataggcttg
    25921 gtcatcaccg gatccaaacc gcacaagccg gtgagggtag gataccagtc gatatgtgcc
    25981 agcggctgat tgatttttct ccccgcttca attttttcag gccaacggat cagtgcaggt
    26041 accctgaccc cgccttcatc aacctgtcct ttgatcccct tcataccgcc attaaatcgg
    26101 actccattgg gcccattgtc ggacatgaaa atcacgattg tgtttttttc gaggcccgtt
    26161 tcttccaagc tcttcaggac acgaccgatg gagtagtcca tatgatccac catgccatag
    26221 agggaagcca gttcatcatc cagtcccatc gccttgtatt tgtcgaacca ggcatccggc
    26281 aactggaaag ggctgtgggg tgcattataa ggcaaataac aaaagaacgg ctggtcttta
    26341 ttccggccaa taaaatcgat agcccggtcc gtcaggatgt cggtaatata accttgcgtt
    26401 ttaacggtat gggacaaact gtccaggccg gtattaaaat aattggtcaa gtgcccggcg
    26461 caaaaaccga taaattcatc aaatccctgg tcttccggcc ggtggggaaa atgtgaacca
    26521 ttgtgccatt tcccaaaaca accggtcctg taaccgttgg ccttgaatag ttcggccagg
    26581 gtcgtttcgt ccgcatccat gacctcatag ccgttggaaa ccgaagtcac cccggtgcgc
    26641 aaatgatacc ttccggtcag aaggctggcc cgtgtgggcg cacacaatgg gctgacaaaa
    26701 aaccgttcaa attcaaggcc ctcctgaatc agccggtcca ggttgggtgt ttgaagtaac
    26761 gggttgccat gagcggacag atccccccag ccctgatcat cggataaaat aaagacgaca
    26821 ttgggttgat cgtcctgccg ggaatcacag cccaaggcaa ggcaaaaagc aaggaacaat
    26881 cctggcacca gattttttat ccttcccata ttttttaaaa gtaatgaaat ccgccgctca
    26941 ataaaaacac tatcttcatt cccatgcccc aacccgcgaa tccatccttt tacccttgac
    27001 cttttgccgt taacctttta tgctaaacct ttctaccctt ttacaggaaa acagccggag
    27061 ataccccgat aaaacagcca tcctttttgg agagtcggcg ctgacttata cccaggtaaa
    27121 cggagcggcc aaccaggtgg ccaacggttt gctgcaactt ggaataaaac ctggcgataa
    27181 ggtggccctg acctgcccaa accttccctg gtttccgatc atctattatg gaatcttaaa
    27241 agccggggcg gtagtggttc cattaagcgt actgctgaaa aaggatgaaa tcgcctatca
    27301 tctcagggac agtgattcca aagcttattt ctgttttaca gggacggatg aaataccgat
    27361 gggcagaatg ggcctttccg ccttcgaaga aacggaggga tgcgaatatt ttttcctgat
    27421 catgcccaaa ccggatatgc cgccgtccat cgaaggatgc aataccctgg ggagtctcct
    27481 tgcagggcaa tcccctcatt ttgataccgt tcaaacgtcc gcggaggata cagcgatcat
    27541 catctacact tccgggacta cgggaaaacc aaaaggcgct gagctcacgc acaataacct
    27601 gatgcataat gccatccttt gtgtggacct gctttacctg gacaaggagg accgtctgct
    27661 gatcgtgttg cctttatttc acatcttcgg aatgacggtg ttgatgaatg cgggaatgtt
    27721 ccggggttgc actgcagtgc ttttgcccag gtttgatgcg ggagcggtgt ttgggctgat
    27781 gcaaaaacac gcgatcaccg tatttgccgg cgttcccact atgtactggg gcctgcttca
    27841 ccaccaggat gataacctgg atgatgacct gatcgcccgg aacctgaaaa tatgtgtttc
    27901 cggaggcgca tcccttccgg tgaaagtgct cgaggatttt gaaacccggt ttaaggttaa
    27961 aattattgag ggctatggca tgtcggaagg gtcccccgta gtcactttca atatgctgga
    28021 ccagggacgt aaagccggtt ccattggcaa acccgtctgg ggggtcgatg ttaaaatcgt
    28081 cgatgaaaaa gaccag
//
LOCUS       DAOQXT010000061        27968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_1978_length_27968_cov_6.771612, whole genome shotgun sequence.
ACCESSION   DAOQXT010000061 DAOQXT010000000
VERSION     DAOQXT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 27968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27968
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_27968_cov_6.771612"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="PKM27_11455"
     CDS             <1..1132
                     /locus_tag="PKM27_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNR07923.1"
                     /translation="SVLGIGIGTAALILVLSVLNGFEDLIKGLFNSFNPDLKITLERG
                     KTFEQDPAEYSALLQTANVRSVSAFLEETALLEYKDIKDFATLKGVDTAYALHSNILT
                     KMDEGVFELVSPLGFQAVAGLGIRNKLAISIDDPFARISAFMPDQKASLVQPFKRRSL
                     IPAGIFSIQQDYDNRYVFVDLGFMQDLAGKYGLITGYEIQLEDYGRKNETVDAIHKIL
                     GSGYKIADRYQQDAAFYKLMKMEKWVSFAILSFVLVLVAFNIVGALWMIVLEKKKDIM
                     VLKSLGMKDGTVGSIFLTTGLWITCLGVLLGFLLSILFYLLQQRFGLVPIPEGFLVDR
                     YPLNLRLTDFLLVGLTVTGIGLCASILPSQKASRLDEPIRYE"
     gene            1309..2130
                     /gene="ygiD"
                     /locus_tag="PKM27_11460"
     CDS             1309..2130
                     /gene="ygiD"
                     /locus_tag="PKM27_11460"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNR07924.1"
                     /translation="MDLKHLMDLPADQEKAMPVLFIGHGSPMNGIEDNEFSQTWKSLG
                     KTIPTPKAVLCVSAHWLTQGTSVTAMAYPRTIHDFGGFPQALYQVKYPAPGSPDLAAE
                     TKKIIRKTTVQLDHEWGLDHGTWTVVRHMYPGARIPVLQLSIDFYKTPEYHYSLAADL
                     QSLRKRGVLIIGSGNMVHNLGMVAWNKLSEPDYGYDWAIEMNSIFKEKIMDGDHQSLI
                     HYQNLNKAAKLAIPTPDHYYPLLYTLGLKTKNDPVRFFNDKLVGGSLTMTGVQLG"
     gene            complement(2132..3295)
                     /locus_tag="PKM27_11465"
     CDS             complement(2132..3295)
                     /locus_tag="PKM27_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07925.1"
                     /translation="MKTVPAFLLVFIFYSMGHTQALFEQGYLVNLSGDSIPCLIKNQD
                     LKKNPQRFEYKLTGESPVQTGDPHNTRAFGIGREVKFIMQEVDVDASSDEIEDLDLER
                     APRFSREKVFLRVLLEGKGSLYELDRRHNVRYYIMNGQGLIEPLVYKAFQVPGKGVAY
                     NFTYRSQLNREMACGSSAKSIQDLKYERTSLVKYVKDYNVCAGGGFVEYEKQARPLKF
                     SARVFTGINFYSALINHRKDPKTGLNVGAELEAILPVNKSKLSVFIAPAYQSFRSDPV
                     EFSLVTAHLELSGLEVPLGFRYYSYLSPKNSLFLEGMFVYDLSFTNAIKFKEITVNEN
                     QDAFVYAFGAGFRSSRFTVQGRWYQTRKFTSVQLIPGQLGFEKISVFLGYRLF"
     gene            3466..4326
                     /locus_tag="PKM27_11470"
     CDS             3466..4326
                     /locus_tag="PKM27_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07926.1"
                     /translation="MRIQILSLFLCSVISLSGQSPQLLQPGNLNSRDPEFGASLPRNE
                     NLILFNRTDSSRMRMSLMQSVRRAKKWLPAEMISFTEPPAWDIDPFISPDGKTIYFSS
                     DRAHPGLAPGFNLWSVSFKKGKWGQPAPLPSAINTVHDEIYCSLARNGNLYFTRMTGP
                     ASGIYFTSLKQSGEAVIHKAKIPVPDSIRLSNPAVAPDESFIVFVSGQLKGSGSADLF
                     ISHRLAFNTWSKPENLGPEINSSFAEFAPFISADGQWLYFTSERPGVVDQFPAGHRRP
                     GDIYKVKLKK"
     gene            complement(4427..4999)
                     /gene="nadD"
                     /locus_tag="PKM27_11475"
     CDS             complement(4427..4999)
                     /gene="nadD"
                     /locus_tag="PKM27_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNR07927.1"
                     /translation="MKTGLFFGSFNPVHTGHMIIANHMAYHIDLQQVWMVVSPHNPLK
                     PSAGLAKDYDRLHLVQLAIGDNPRLRASNVEFSLPKPSYTIDTLTYLKEKHPEHEFVL
                     IMGGDNLATLNKWKNYEKLLEWYEIYLYRRPRVEDGPFIHHPHVKLVEAPLIDLSATY
                     IRECIKKGLSVQYLVPDTVYEYLRGSSMYR"
     gene            complement(5108..6229)
                     /gene="dnaN"
                     /locus_tag="PKM27_11480"
     CDS             complement(5108..6229)
                     /gene="dnaN"
                     /locus_tag="PKM27_11480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNR07928.1"
                     /translation="MKFSVSSAELLKQIQLAGGAIGTNPVLPILEDFLFQVKGKILTI
                     QSTDLETSVKTEMEVKSDQNGAVAIPAKILIDTLKSLPEQPVTFSVDTATNSIEITSS
                     YGRYRLAGENPGEYPSFPSTVTQDTVKINSGILHDAFQKTLFATSSDELRLAMTGILV
                     QLDFNKIIFVSTDAHKLVKYVFQGVSSDVATQFILPKKTCSLLNNILPPDTEIEISFS
                     KSNAFFSFGHSLLACRLVDAKYPDYNGVIPVDNPNVLTVNRTQLLQSLRRIAIYANKT
                     TNQVALQVDEGSLTITSQDLDFSNEATEQLACTFNGKPMSIAFNGKFLIEMLSVMSTD
                     EISIRLSTPNRAGILTPAEEIKGQDLLMLIMPVLINQGH"
     gene            6529..7800
                     /locus_tag="PKM27_11485"
     CDS             6529..7800
                     /locus_tag="PKM27_11485"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNR07929.1"
                     /translation="MPANPMRYLPAGNPFSVPQPKFEKLVLANKAEFYPLNRGLVPAL
                     KLELTFPAGRPFEDKIGLASATLQGIKGGTLKRSATRLAEGFDYWGSSLNFDFYIDTC
                     GIKLFVLEKYLPRVLPLLVEMLSSPSFPEGELRLYKRQQVEKLRSDMTRADVVAYRKF
                     TEILFGPGHPYGYNSTVESIEEIHREDLISHYRSHYDMKRCKIFLSGQFSLQTRDLVA
                     HVLGSVELNEGHGRPHIPSRKLPLPSQNRISLEYAVQSSIRMGRVLFRRNHPDYVKMF
                     VLNTLLGGYYGSRLMQNLREKKGLTYHIYSSLDTFLYGGYFLISTEVDKERSELALQA
                     IRQEIEKLQTRLVGSGELRMVKNYLRGSFLHYFENAFAYGELIRTLSLEGGLGAYQKL
                     VDGVEKVTPEELRDLAIHYFQEKDLSLVQVS"
     gene            7952..8983
                     /locus_tag="PKM27_11490"
     CDS             7952..8983
                     /locus_tag="PKM27_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNR07930.1"
                     /translation="MKWFNFFSQELAIDLGTANTLIMQNDQIVIDEPSIVAINRKTGE
                     TIAVGSRAMQMHEKTHENIKTVRPLKDGVIADFQAAEKLIEGLINMIGTKRSFFTHLK
                     MVICIPSGITEVEKRAVFDSADHVDSKETYLIHEPMAAALGIGLDVEEPVGHIIIDIG
                     GGTTEIAVIAMSGIVTDQSIRTAGDEFNYDIMEYMKRAHNILIGERTAEQLKINVGSA
                     LKQLENAPEDYPVNGRDLMTGIPKQITIGHTEIATALDKSIAKIEDAILKALESTPPE
                     LAADIYKNGLYLTGGGALLRGLDKRISAKTKLPVKVVDDPLRAVVRGTGLALKNSDRY
                     SFLIDRKSV"
     gene            9000..9827
                     /gene="mreC"
                     /locus_tag="PKM27_11495"
     CDS             9000..9827
                     /gene="mreC"
                     /locus_tag="PKM27_11495"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNR07931.1"
                     /translation="MQNLFAFFYRYAFVFLFLILEFVSIRLIVSRNENQRTIFLNSSN
                     LIVGTLYERMDAVRKYFGLRQDNAALAEENSRLKNQLANLMNASPLITDSITDTVYRQ
                     RFVMHAAQVVNNSIERRNNVMTINKGARDGIDKYATVVEPTGIVGFVTHLGIRYATVM
                     SILNSNSRVSAMVKRTSTRGNLVWPGNSPLIMNVEAIPKHADVRVGDTIVTSSFSHFP
                     AGHPVGEITKATIEPGENFYLIEVRLFNDLARTNHVYVVNDLHKSELDSLNQFNTQK"
     gene            9824..10342
                     /locus_tag="PKM27_11500"
     CDS             9824..10342
                     /locus_tag="PKM27_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07932.1"
                     /translation="MNSIGFRHGLRLVFLFFFQVLVLKQVHLNWGDFNYISIILYPLW
                     ILLLPVQTARWILVAGGFLAGLGVDWFYDSPGVHASAATFTGFLRPFILSYLEPRGGY
                     NRVPIPSKAHLGLNWFVTYASIMLIGYLFIYISMEVFTPALLGTILVKTLFSFIISIL
                     MVLLYMFIFDPK"
     gene            10339..12213
                     /gene="mrdA"
                     /locus_tag="PKM27_11505"
     CDS             10339..12213
                     /gene="mrdA"
                     /locus_tag="PKM27_11505"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNR07933.1"
                     /translation="MKGNARHIIEATVIILAGILLFQSFYIQFLDKDIRKRAQYAALE
                     KVVSYPARGAIYDRNSKLLVYNNPVYDIEALYRKIDKNMDTARFCQLLGIDRATFISE
                     LNKDWSNKQFSKSKPFPFIKRVPVDQFAGFQEMMHEFPGFSAQLRYIRGYPRPIAAHA
                     MGYISEVDPAEIEKSNGVYERGDYKGTSGIERAYESQLKGDKGYKYLLKDNLGVTVGS
                     YQNGALDSAARSGDELMVSLDADLQEYGERLMQNKRGSVVAIEPSTGEILCLISAPTY
                     NPNLLTVDRDRGKYFSALVQDTLKPLFDRTVQAQYPPGSIFKTVVALAGLQMGVLDPN
                     KGTHCGGSYTLGRVYKCSHIHYDPRNLGDALEQSCNVYFYQKIQDIMNKYGSDKPGKG
                     LDEFAGYMRQFGLGKMLGMDYFNELPGTIPTDAYYKRHYYRLFRDSTWKAAYEISIGI
                     GQGVIQLTTAQMANLCAAIANRGYWITPHFYKGVIKNHQLQPQDKFLEKHKIDIDPRH
                     FETVIDGMERAVLTGTARIAQLKGIEVCGKTGTAQNPHGADHSVFCAFAPKDNPKIAI
                     AVIVENSGFGSTFAAPVASLLMEKYINGKVDSTRTWLEKRTLEQDLIHKPQPAPPTKP
                     "
     gene            12222..12731
                     /locus_tag="PKM27_11510"
     CDS             12222..12731
                     /locus_tag="PKM27_11510"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNR07934.1"
                     /translation="MIVSAIVAMNQDSAIGVKGQIPWYLPADLKYFRKTTTPHHVIMG
                     RQTFESIGKPLPNRINIILTRDPFYLSTGCLIAHSIREALDISADHGEKEAFIIGGAQ
                     IYQAALPLLDKLYITRVSLEVPEADAFFPTIDYKEWTLDSSVAFPADDKNKWPYCFEV
                     YSRVSKLAS"
     gene            complement(12753..13421)
                     /locus_tag="PKM27_11515"
     CDS             complement(12753..13421)
                     /locus_tag="PKM27_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07935.1"
                     /translation="MKLNSVLIFLILLAVSSRAQVMESSKVMSKGQQTSLNLTIPDVS
                     AEYVDDVYKEFIKTYKGKTKKDKKSNEWYSDDAQVASIANGAPIDIYARIESAGNQAT
                     VTLWIDLGTAYISSGTYPREYEEAGKLLEKFGQQVKISQAEDELAAVERDMKKLDADM
                     KKLKKDNEDYHKEIERANEKIKEAEKNILKNEEDQKSKQTAIEDQAGKVEAAREKVNK
                     LKQM"
     gene            complement(13473..14396)
                     /locus_tag="PKM27_11520"
     CDS             complement(13473..14396)
                     /locus_tag="PKM27_11520"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNR07936.1"
                     /translation="MGPLVFIGNPEAGKGRTLRAWPRLAEILKSAGIPHEIRWSARKD
                     HARELACQAMKEEPGLIVGFGGDGTAHQVVNGMVDSGIQNKTAFTMFPVGTGNDWARY
                     WKIPFKIHEWVEMIKQGNTHEHDLGLIVYTDEQGQEKKQVFNNVAGAAYDAYVVDFIE
                     NRKKKMSAGGIQYLWFIFRCLFSYRLQNSRISWPGGREADRFYTINIGICPYSGGGLH
                     LVPHAHPQSGALAVTAVRPVSKLQVFLLSPLFYSGKIIRHPRVLHFNTESLILEAGPG
                     EIIKIEAEGELLGNFPARISVIKKGLKICAP"
     gene            complement(14457..16718)
                     /locus_tag="PKM27_11525"
     CDS             complement(14457..16718)
                     /locus_tag="PKM27_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR07937.1"
                     /translation="MSLILRLASLYVLLIIGSSLPAQNREAGIFGKITDKTSGLPISD
                     ALVRIREKEGSHALSDEQGRFRLAAVPAGKYTLEIRALGYEFFSLEIQVDGPQHLDFQ
                     LQPGTIELHEVVVESSEAGALPRSFSALDKLLRPVNSAQDLLQLIPGLFIAQHAGGGK
                     AEQIFLRGFDSDHGTDFYIAVDGMPVNMVSHAHGQGYADFHFVIPETIDRLSVYKGPY
                     TTRFGDFSTSGTGEFMTRNGLDRSLVKAEAGMFNTARLVGLFDLLPGTAPNKEQHAYA
                     AAEYVYTDSYFDKRQKFHRYNLFAKYHGRISDRTFLQLSGSAFSAEWNGSGQIPERAV
                     LSGQISRYGQIDSFEGGNTSRKNLNAILSTSMPRNGLMKHQLFYTDYDFSLFSNFTFY
                     LNDPLYGDEIHQTDHRSILGYLGSYQHHSSFWGRNLLSQFGLASRYDHTGVRLLHSRR
                     REDLEPLVSGKVSQLNAGAYADFSFEIDRRWTLNAGTRLDLFSFAFKETGAAEGKKRL
                     IGRLSPKLNLYYQADARSRFFIKSGLGFHSNDARSVVLGQTENSLPKALGYEAGTELK
                     LGRKVLMQMALWALHSQSELVYVGDEGVVETNMPTRRMGLDLGLRVQPANKWFADADF
                     NVNHGRLVGAPEGEDFIPLAPRVTSTGGLQYKQDRGFNASLRYRLMDSRPANESNSIR
                     AKGYTILDASAMYVFKKMELGFTIENLLNREWNQAQFDTTSRLFDEGQAVSELHFTPG
                     TPFFLKGVVVYRF"
     gene            16894..18276
                     /locus_tag="PKM27_11530"
     CDS             16894..18276
                     /locus_tag="PKM27_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNR07938.1"
                     /translation="MQPFIIIFSLISLSLYSQQSPSFIFGLHQQYLETSITSKKFKHR
                     VLDSLIQQVAKDPLFEVKTAGQSFLGKNIYLIRAGTGSRRVLLWSQMHGDESTATMAL
                     MDLFNYLQDESQVGRKLLLEHCTLYFIPMLNPDGADLFVRRNAQQIDVNRDALFLQSP
                     EGRLLKSIRDSLNPEFGFNLHDQNKYLAAGDSKRPASISLLAPAYNPEKDINPVRERA
                     MLLAAQIHLTLQAFIPQGIGRYDDEFEPRAFGDNIQKWGTSTVLIETGGYENDPEKQY
                     LRKINFTAILSALESIATNSFHHQNIRDYWSIPENKSRLNDLIIRNVRLELNGKEYLS
                     DLSFNIPESSGRATLVDMGDLSIFRGYREWDGTGKKLVPGKVYPRMIKNSRKLGRLDQ
                     VRLIKDGYTEVRLRKPGPKTQKQLLNVLGATEVKNNTLQPGANPNALIYEKEEVIYLL
                     SNGNLVEIRP"
     gene            18316..19743
                     /locus_tag="PKM27_11535"
     CDS             18316..19743
                     /locus_tag="PKM27_11535"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNR07939.1"
                     /translation="MEKISVFDIFKIGIGPSSSHTMGPWRAALRFIHELKALNLLEKV
                     QGIQVDLYGSLAKTGHGHGTDTAILLGLSGFDPVTIPEESIPQEVERIHRSGRLSLEG
                     PATIGFDPGKDLIFHYFESLPYHPNGLRFKASVQGLAPYTSTYFSTGGGFVLKEKEEA
                     DRPEVHLPYPIQTSRDLESYCRLLQKPIDEIVLQNERMFRQEEEVHLGLLNIFETMMQ
                     CAYKGCHTEGVLPGGLNVSRRAASINKKLLDPETSYSDARGWLDAVRRSGHDFETINK
                     WISCFALAVNEVNASFGRVVTAPTNGAAGVIPAVLLYHICFGKGKEDKDRDIIRFLLT
                     AGEIGSIFKKGATISAAMGGCQAEIGVSSAMAAGGLALCLGGDYRQVLMAAEIAMEHH
                     LGMTCDPVGGLVQIPCIERNTMGAIKAITAAHLALESDPEQAKVSLDEVIRTMWLTAL
                     DMNSKYKETAEGGLAVNIAVNVAEC"
     gene            19905..20537
                     /locus_tag="PKM27_11540"
     CDS             19905..20537
                     /locus_tag="PKM27_11540"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNR07940.1"
                     /translation="MLAYLSLGSGFLSLLSLLILHFVSPEFKPSWRMISEYALGKHKW
                     LITSFFVFWSLSTFFLAFLLWNQVGGIWARIALILLVVSGIGEFMGGAFDVKHKLHGL
                     AFALGVPALPVAALVLSYQLVRQDAWAIHKSSILYSAHSTWISLVLMAVTMIVMISGF
                     KKAGIPMGPDIEPPDSVPAGVIAVSGYANRILVFCYILWLLVIAKIFTTQ"
     gene            20613..21179
                     /locus_tag="PKM27_11545"
     CDS             20613..21179
                     /locus_tag="PKM27_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNR07941.1"
                     /translation="MRKLILQMQTSLDGFVATGPNDEQSWVTWALDEIKPHVIELLDS
                     TDTILIGRKLAVDYIPFWQDTYTKPEDPMFEFAKRIVAAKKIVFSRTLEQSIWPNTEL
                     AKGPLAEEVNALKNQDGKDLVVYGGASFVSALIKEGLIDEYQFYINPVVLGKGVPVFD
                     LIDSFRPLVLIKSMVFPGGIILTKYQPK"
     gene            21321..23822
                     /locus_tag="PKM27_11550"
     CDS             21321..23822
                     /locus_tag="PKM27_11550"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNR07942.1"
                     /translation="MNKGLSSKEVADRIARFGYNELPTSKSKNILHIALEVIREPMFI
                     LLISCGSLYIILGDYTEGIILVCWVFIIIFITFYQHQKTESALDALKKLSSPRALVIR
                     DGKEVRIAGREVVPDDVVILNEGDRVPADGILSEGLNLTIDESLLTGESLPVTKTTHN
                     EKEPNIPVYSGTLVVQGKGLMEVKTTGSNTEFGKIGKSIQHIEQDQTRLQKEMKSLIR
                     NLFIAGALLSVVVIAGFYFTRGNFVQSLLNGLATAMAMLPEEFLVVLTVFLALGAWRL
                     SQKNVLTRKPSAIETLGSATVLCSDKTGTITQNKMELASLFANNKMFNKSSFREKAPE
                     IRELLVHAFHASQKDTIDPMEKAIGKGYNEWVIPKALLPDLVKEYPLSTRLFAMTRVL
                     KPKHEDHYLVSCKGTPEAVFSLCKMNDTEVSEHLTTVQKMAEQGQRVLGVAKAAWNKK
                     EIPDTQQAYEFEFIGLIGFEDPIRPEVPRAIQECHEAGIKVIMITGDYPSTAKCIASQ
                     AGMNTDGVVLTGTDLKNMSESELAEKIKSVNIFARIVPEQKLQIINALKTNGEIVAMT
                     GDGVNDAPALKAADIGIALGGKGTDVAREASSLVLLDDHFSSIVVAIRSGRKIFDNLQ
                     KSMSYIIAIHIPIIGLTLLPAFFPGLPILLMPLHIVFLELILDPVCSIAFESEKEEKD
                     IMKHPPRDPEEPFFGFGKIVGSASVGLLLMAMVLAIYFLSLQERHTDGETRAIAFSSL
                     ILGNIFLILTTISKTRNYIDVLLEKNMALLVILVIATALMIMVIYHPYLRTVFSFDYP
                     GFSHFNLAIAGALAVLFILETIKMIKQNFIMRTPV"
     gene            23875..24333
                     /locus_tag="PKM27_11555"
     CDS             23875..24333
                     /locus_tag="PKM27_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07943.1"
                     /translation="MKRFLFLTLFNALCVLFAYAQGISNEDIPDKKEIYSLIDQYSSA
                     REKRDTAMLRHLLTPDADQLVSSGQWRVGIGEAVKGMLLSSESNPGSRRLIVDKIRFL
                     DSKHAIADARYEIQNPDGAARKMWSTFIVVFQNGSWKIAAIRNMLPAPAR"
     gene            24469..25440
                     /locus_tag="PKM27_11560"
     CDS             24469..25440
                     /locus_tag="PKM27_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07944.1"
                     /translation="MNKLVFFFCLIPSGIFGQAANPLKGYPGIRDGVFVYFPPSRAML
                     LLGGGKSSPNSSESNVWKWKDSQWAMIPASGPGSRDFFPCAYHPGSGNIYCYGEQDSP
                     GGVFWSFDGAQWKSTKITGLESRDHHQMVYADHLNAFIIYGGANERGILDTLTWLVRD
                     GKATALNIPGPGIRYHSAMTYDKQRKKVVLYGGGKKPWEHWEFDGLRWVKINSRTSPG
                     NKFYHFMAYDESSDKIILHGGWRNQDPFDPVNKQNPQTWAWDGQDWTNIAGESIFSIA
                     FGYDAGRKQVVTYGRSGGPDNAPLALWILENGSWKKISDYGMPNTND"
     gene            25506..25877
                     /locus_tag="PKM27_11565"
     CDS             25506..25877
                     /locus_tag="PKM27_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR07945.1"
                     /translation="MKLGAFSVSLSVRDLRASREFYEKLGFSVFAGDPGKNYLIMKNG
                     NALIGLFHGMFEKNILTFNPGWDENAHPLDEFDDVRKIQRELKNNNIDIIQAADEETV
                     GPASFMVVDPDGNPILFDQHR"
     gene            complement(25888..26619)
                     /locus_tag="PKM27_11570"
     CDS             complement(25888..26619)
                     /locus_tag="PKM27_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07946.1"
                     /translation="MKTNRFLYDYSVVAFVLFFAFALWAFWPSYYSHLGDQEEVRFHT
                     HGIAMTLWCMMLIGQAWLIRKKRFVLHRRLGRLSYILMPVLLILTLNLIHHQLSGRGG
                     WNPLSLYSLALMVNATIVLAILYGLAIYHRRRPLLHARYMLATVFPLFTPVTDRLIYA
                     HFRPLLNYLPVLEGRPIAPVAGFLLADLLLLFLVYWDWKTHQRLDVFPKVLALLVGYH
                     FSVLSFYRFGFWERFGNWFLSLPLS"
     gene            26950..27531
                     /locus_tag="PKM27_11575"
     CDS             26950..27531
                     /locus_tag="PKM27_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNR07947.1"
                     /translation="MKKPLLIQNQKFEKTGQASVALETGEYRECTFNQCDFSNADFTD
                     CIFSDCEFTGCNASLCKLTRTGFREVRFKNCKLLGLHWEDCNSLIYNLTFDHCTLDFS
                     SFYNLNLRETVFRHSSLKESDFTDADLSRAVFESCDLDRTIFYHTNLEKADFRSARFF
                     TIDPEQNRLRGAKFSAGGLPGLLKKYGLEIDGE"
     gene            27567..>27968
                     /locus_tag="PKM27_11580"
     CDS             27567..>27968
                     /locus_tag="PKM27_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07948.1"
                     /translation="MEMHFPAPPLGQFIESFLYYKDYQPGHLIDRFFPDGNIYAVIDL
                     NEEPQYIFDNQTLRPIQTCRKVWFSGLRTNYISIPSCGESELFVINFRKGRAFPFVKG
                     PLGEYTDAVVDGGQSFRKDILRVREALLPLPG"
BASE COUNT         7063 a         7168 c         6940 g         6797 t
ORIGIN      
        1 gtcggttttg gggattggga tagggaccgc tgccctcatc ctggtcctct ccgttttaaa
       61 cggctttgaa gatttgatca aagggctgtt caactcgttt aacccggacc tgaagatcac
      121 ccttgaacgg gggaaaactt ttgagcagga tcccgcggaa tactcggccc tgttgcaaac
      181 cgcaaatgtg cgctcggttt cggccttcct ggaagaaaca gccctcctgg aatacaagga
      241 catcaaggat tttgccaccc tgaagggagt ggatacggct tatgcccttc attccaatat
      301 cctgaccaaa atggatgaag gcgtcttcga attggtttct ccgctcggtt tccaggcggt
      361 ggccggattg ggcatccgca acaaactggc catcagcatc gacgatccat ttgcccggat
      421 ttccgccttc atgcctgatc agaaggcaag cctggtccaa ccgtttaaaa gaagatcgct
      481 catcccggca ggcattttca gcattcaaca ggattatgac aaccggtacg tcttcgtgga
      541 cctgggcttt atgcaggacc tggccggaaa atacgggttg attaccggct atgaaatcca
      601 gttggaagat tacggccgga aaaacgagac cgttgacgcc atccacaaaa tactgggaag
      661 cgggtataaa atcgcggacc gttatcagca ggatgcggct ttttacaaac tgatgaaaat
      721 ggagaaatgg gtgtcttttg ccatcctgag ttttgtgctg gtcctggtgg cctttaacat
      781 tgtcggggcc ctctggatga tcgtccttga aaagaaaaag gacatcatgg tactcaagtc
      841 cctgggcatg aaagacggca ccgtcgggtc cattttcctc accaccggcc tttggattac
      901 ctgcctgggt gtcctgctgg gtttcctgct ttccatcctt ttttacctct tgcagcaacg
      961 ttttggactc gtgcctatcc ctgaaggatt tttggtggac cgttaccccc tgaacctgag
     1021 gttgaccgat tttctgctcg tgggccttac cgtcacaggc attggcctgt gcgcatccat
     1081 tcttccttcc caaaaagcct cccgcctgga cgaacccata cgctacgaat aaaaaaagat
     1141 tgtcgtattg ctacgacaat ctcccaatag gtgatagggc agtcgcttaa gcgtgaacaa
     1201 cctcacctgc actctgtgta tccaacgccc aaaatgtcac ctaaaacaat aaaaaatatt
     1261 tcaacatttg cagattggta tttgtttatg ctataattgt tctactgcat ggatttaaaa
     1321 catcttatgg atcttccggc ggaccaggaa aaggccatgc cggttttatt tatcggccat
     1381 gggtcaccca tgaacgggat tgaagacaat gaattcagcc agacctggaa atccctgggt
     1441 aagaccattc ctacccccaa ggccgtcctt tgtgtttcag ctcactggct gacccagggg
     1501 acctcggtga ctgccatggc ctatccgcga accatccatg atttcggcgg attcccccag
     1561 gcgctttacc aggtgaaata ccctgccccc ggcagtcccg acctggcagc agaaaccaaa
     1621 aaaatcatcc gtaagactac ggtccagctg gaccacgaat ggggcctcga tcacggcacc
     1681 tggaccgtgg tcaggcatat gtatcccggg gcacgcatcc ccgtgctgca gctgagcatt
     1741 gatttttata aaacgccgga atatcattat tcccttgccg ccgatctgca gtccttgcgt
     1801 aaaagagggg tgctgatcat cggcagtgga aacatggtcc acaacctggg catggtcgcc
     1861 tggaataaat tgtccgaacc cgattatggc tatgactggg ccatagaaat gaattccatt
     1921 ttcaaggaaa aaatcatgga cggggaccat cagtccctga tccactacca aaacctcaac
     1981 aaggccgcca aactggccat tcccaccccg gatcactatt atcccctcct ctataccctg
     2041 ggcctgaaaa caaaaaacga tccggtccgt ttttttaatg acaaactggt gggcggctcc
     2101 ctgacgatga ccggggtaca gctcggttaa attaaaagag gcggtagccc aggaaaacag
     2161 aaattttttc aaagcccagt tggcccggga tcaattggac agaagtaaat tttctggttt
     2221 gataccacct gccttgtacc gtgaaccgtg aacttctgaa tcctgccccg aaggcataaa
     2281 cgaaggcatc ctggttttca ttgactgtaa tctccttgaa tttgatcgcg ttggtaaaag
     2341 aaaggtcata aacaaacatc ccttccagga acaggctgtt cttaggactt aaataagaat
     2401 agtatctgaa gcccagggga acttccagcc cactaagttc aagatgcgcg gtgaccagtg
     2461 aaaattctac ggggtctgaa cggaaggatt ggtaggcagg ggcgatgaaa accgacagtt
     2521 tggatttatt taccggtaaa atggcttcca gttccgcccc tacgttcagc ccggtttttg
     2581 gatccttgcg gtgattgatc agcgcggaat aaaaattaat cccggtaaac acccgggcgg
     2641 aaaactttaa gggtcttgcc tgtttttcat attctacgaa tccgccgccc gcacaaacat
     2701 tatagtcctt tacatatttt accagggagg ttctttcgta tttcaaatcc tggatggact
     2761 tggcagaaga tccacaggcc atctcccggt ttaattggct ccggtaggtg aaattataag
     2821 ccaccccctt ccccggaacc tggaaggcct tgtaaaccag gggttcaatc agaccctggc
     2881 cgttcatgat ataataacgg acattgtgcc gccgatccag ttcatataag gagcccttgc
     2941 cctccaggag taccctgagg aatacttttt cccttgaaaa ccggggggct ctttccaaat
     3001 ccaggtcctc aatctcatcg ctggatgcat cgacatccac ttcctgcatt ataaatttta
     3061 cttcccggcc gatgccaaaa gcccgggtat tgtgcgggtc cccggtctgc accggactct
     3121 ccccggtcag tttatactca aaacgctgtg gatttttttt caagtcctga tttttgatca
     3181 ggcagggaat gctgtcgccg cttaaattca ccaggtaacc ctgctcaaat agggcctggg
     3241 tatgccccat ggaataaaat ataaatacaa ggaggaaagc agggactgtt ttcatagcgt
     3301 gtgattgaaa aaccaattta gaaaaaattt ttaaaattcc aaattgcgta tgggcagggt
     3361 cttgggccgt ctctcgcctg cgggagcttc ctgcctcttt taccaaaaag ttaatcccgc
     3421 cggatgggtt attcggacgg agagctcagg taataatttt gcccgatgcg aatccaaatc
     3481 ctctctcttt tcctttgcag cgtaatatcg ctttccgggc aaagtcccca attgcttcag
     3541 cccggaaacc tcaacagccg ggatcccgaa tttggagcca gtcttccacg gaatgaaaac
     3601 ctgatccttt tcaaccggac ggactcttcc cgtatgcgaa tgagcttgat gcaatccgtt
     3661 cgcagggcca aaaaatggtt gcccgctgaa atgatttcct ttactgagcc cccggcctgg
     3721 gacatcgatc ctttcataag cccggatggt aaaacgattt acttttcctc cgaccgggcc
     3781 catcccggcc tggctccagg tttcaacctt tggagcgtat ccttcaaaaa agggaaatgg
     3841 ggccagccgg cccccctccc cagcgcgatc aatacggtac acgatgagat ctattgttcc
     3901 ctggcccgaa acggcaatct gtattttaca agaatgacag gacctgcttc cggcatttat
     3961 tttacctcat taaaacaatc cggggaagcg gtgatccata aggcaaagat cccggttccg
     4021 gattccatcc ggctcagcaa tccggccgtg gcgccggatg aatcgtttat cgtatttgtt
     4081 tccgggcaat tgaagggttc aggcagcgca gacctgttta tctcccatcg cctcgcattt
     4141 aatacctggt ccaaacccga aaatctcggc cctgaaataa attcatcctt tgctgaattc
     4201 gctcctttca tatcagccga cgggcaatgg ctttacttca cttccgagcg tccgggagtg
     4261 gtggaccagt ttcctgccgg ccatcgaaga ccgggagata tttacaaggt caagttgaag
     4321 aagtaaaaat aaaaatctgt gctctcctgc gctcgcgtta tgaaccggtt ctactgaata
     4381 aaaagacctc cccttatcgc tgatactggt tcccggataa gccttttcac ctgtacatag
     4441 acgatcccct gagatattcg tacacggtat ccggcaccag gtattgaacg ctcaacccct
     4501 ttttgatgca ttcccggata taggttgcgg aaaggtcgat caggggcgcc tccaccagtt
     4561 tcacatgcgg gtgatggatg aatggcccat cctcaaccct gggtctccgg tacaggtaga
     4621 tctcgtacca ttccagcagc ttttcatagt ttttccattt gttcagggtg gccaggttgt
     4681 ccccgcccat gatcagcaca aactcatgtt ctgggtgttt ttctttcagg taggtgaggg
     4741 tatcaatggt ataggaaggt ttgggcaggg aaaactccac atttgacgcg cggaggcgcg
     4801 gattgtcccc gatggccagt tgtacgagat gcagccggtc gtaatccttg gccaagccgg
     4861 cggaaggttt taaaggattg tgcggagaaa ccaccatcca gacctgctgc agatcgatgt
     4921 ggtaagccat gtgattggcg atgatcatgt ggccggtatg caccgggttg aaagatccga
     4981 aaaaaaggcc cgttttcatt taccgcggct ggatgggaaa caaggtttat tcttattgga
     5041 ccctctttcc ccggtatggg aaagctcagg atccattacc cttgccggga ccctggaggg
     5101 acccatgtca atggccctgg ttgatcagta ccggcatgat gagcatcagc aggtcctgtc
     5161 ccttgatctc ttcagcgggg gtcaggatgc ccgcccggtt gggagtggaa aggcggatgc
     5221 tgatttcatc cgtggacatc acgctcagca tttcaatgag aaatttacca ttaaaagcga
     5281 tggacatcgg ttttccatta aaggtgcagg ccagttgttc ggtcgcttca tttgaaaaat
     5341 cgaggtcctg gctggtgatg gtcaggctgc cttcatccac ctgcagggct acctggttgg
     5401 tggttttatt ggcatagatg gcgatgcgcc tcagggattg cagcaattgg gtccggttga
     5461 ccgtcagcac attgggattg tccaccggga tgaccccgtt atagtccggg tattttgcgt
     5521 caaccagccg gcaggccagc aggctgtgac cgaaagagaa gaaagcgttt gacttgctga
     5581 aggagatttc gatttccgta tccggaggca agatgttgtt cagcagggaa caggtttttt
     5641 tgggaaggat aaattgggtg gctacatcgc tggacacccc ctggaatacg tatttgacca
     5701 gcttatgcgc atcggtggag acaaagatga ttttgttgaa atccagctgg accaggatgc
     5761 cggtcatggc cagccggagc tcatcgctgc tggtggcaaa caaggttttt tgaaaagcgt
     5821 cgtgcaggat cccggagttg attttgaccg tatcctgggt gaccgtggat ggaaaactgg
     5881 gatattcccc ggggttttcg cctgcgaggc ggtaacggcc ataactcgaa gtgatctcta
     5941 tgctgttagt ggctgtatcc acggaaaaag taaccggttg ttccggcagt gatttcaggg
     6001 tgtcgatcag gatcttggcc ggaatggcca cggccccatt ctggtcggat ttgacctcca
     6061 tctccgtttt taccgaagtc tccaggtccg tggattgaat ggtcaggatt tttcctttga
     6121 cctggaaaag aaaatcttcc aggatgggaa ggaccgggtt ggtgccgatc gcaccaccgg
     6181 ccagttgaat ttgtttgagc agttcggctg aggagacgct gaatttcatg aaggcggatt
     6241 atttaattcg ggggcaaaat taaccaaaac cccgaaggca gccaatcttt aatgactccc
     6301 ggttctcccg ggacatctct cttgagaaag aactaaggac ccgtctgcct gctcaggcag
     6361 gtaaaaaatt tccatagagt cccgggccga atagcgaggc cgcggctggg ttcaaccggc
     6421 ccacggcccc atgagaataa taaacagcgt aagcgtaatc cttaaaaaga tgaattccag
     6481 ggatcctcca tcatttgagc gacaggaatt gtgcaatacc ctctattttt gcctgccaat
     6541 ccaatgcgct atctgcccgc aggaaatcca ttttccgtcc ctcaacccaa attcgaaaaa
     6601 ctggttttag ccaataaagc cgaattttac ccgcttaaca gaggactggt cccggctctc
     6661 aaactggaac tcaccttccc cgccggccgc ccatttgagg ataaaatcgg acttgcctcc
     6721 gccaccctgc aggggataaa agggggcacc cttaaacgat ctgccacccg cctggccgaa
     6781 ggattcgact actggggctc ctcgctgaat tttgattttt atatcgatac ctgcggcatc
     6841 aaactttttg tgctggaaaa ataccttcca agggtcttgc ccctcctggt cgaaatgctc
     6901 agcagcccct ctttccctga gggtgaactc cggctgtata aaaggcagca agtcgaaaaa
     6961 ctgaggagcg atatgacccg tgcggatgta gtggcttacc ggaagtttac cgaaatcctg
     7021 tttggtcccg gtcatcctta cggatacaac agtacagtgg aaagcatcga agaaatccac
     7081 cgggaggacc tgatttccca ctaccggtca cattatgaca tgaaaaggtg caaaatcttt
     7141 ttatccgggc agttttctct tcaaacccgg gacctggtag cccatgtgct gggctctgtc
     7201 gaattgaatg aaggccacgg caggccccat atcccaagcc gcaaactgcc cctgccttcc
     7261 caaaacagga taagcctgga atacgcggtc cagtcctcca tccggatggg cagggtcctt
     7321 tttcgccgga accatccgga ttatgtaaaa atgtttgtgc ttaataccct gcttggcgga
     7381 tattacgggt ccaggctgat gcaaaacctg agggaaaaaa aaggcctgac ctatcatatc
     7441 tattcttcgc tggatacctt cctttatggg ggatatttcc tgatctccac ggaggtggac
     7501 aaagaaagga gcgaactggc cctgcaggcc atccggcagg agatcgaaaa attgcaaacc
     7561 aggcttgtag ggagcgggga gttgaggatg gtcaaaaatt acctccgggg cagtttcctg
     7621 cattattttg aaaatgcctt tgcctatgga gaactcatcc gcaccctctc actcgaaggg
     7681 gggcttgggg cttaccaaaa actggtggac ggggtggaaa aagtcacccc ggaagaactc
     7741 cgggacctgg ctattcatta ttttcaggaa aaagacctca gcctggtcca ggtgagctga
     7801 ttttctttaa atctttggaa ttaaatttat tatctctggc ctaatttcta atatatttgc
     7861 ttcctttgcg tttcaatcct gtgaatttct gaaagttaaa attttaggca aagtttttgc
     7921 aaggatatta taatagggat aagcattacg aatgaaatgg tttaattttt tttcacagga
     7981 gctggcgata gaccttggaa cggccaatac cctgatcatg caaaacgatc agatcgtgat
     8041 cgacgagcct tccattgtgg ccatcaaccg gaagaccggg gaaaccatcg cggtcggatc
     8101 cagggcgatg cagatgcatg aaaaaaccca cgaaaacatc aaaaccgtga gacccctgaa
     8161 agacggcgtg atcgccgatt tccaggcggc agagaaactg atagaagggc tgatcaatat
     8221 gatcggcacc aaaagaagct tttttaccca cctcaagatg gtgatctgca tcccctccgg
     8281 gatcaccgaa gtggaaaaaa gggcggtctt tgattcggcc gaccatgtgg actccaagga
     8341 aacctacctg atccatgaac ccatggccgc cgccctgggc atcgggctgg atgtggagga
     8401 accggttggc catattatca tcgacatcgg tggcggcacc acggaaatcg cggtgatcgc
     8461 catgtcaggc atcgtgaccg accagtccat ccgtaccgcc ggcgacgaat tcaactacga
     8521 tatcatggag tacatgaaac gagcccataa tatcctgatc ggtgaaagga ccgccgaaca
     8581 acttaaaatc aacgtgggct ccgctttaaa acagctggaa aacgccccgg aagattaccc
     8641 ggtaaacggc cgggacctga tgaccggcat tcccaagcag atcaccatcg ggcataccga
     8701 gatcgccacc gccctggaca agtccatcgc caaaatagaa gacgccatcc tgaaagcgct
     8761 cgaatccact ccgcctgaac tggctgccga tatttataaa aacggacttt acctgaccgg
     8821 gggcggcgcc ttgctgcgcg gcctggataa aagaatttcc gccaaaacca aattaccggt
     8881 aaaagtggtg gacgacccgc ttcgcgccgt ggtcagaggt acaggactcg ccttaaaaaa
     8941 ctcagaccgt tattcttttt tgattgaccg gaaaagtgtc tgatctttga ggccggaata
     9001 tgcaaaatct ctttgctttt ttttaccggt atgcctttgt ttttttattt ctgatcctgg
     9061 aattcgtcag tatccgcctg attgtcagcc ggaatgaaaa ccagcgcacc atttttctca
     9121 attcctcgaa cctcattgtg ggcacccttt acgaaaggat ggacgcggta aggaaatatt
     9181 tcgggctccg gcaagacaat gccgccctgg ccgaagaaaa ttccaggctc aaaaaccagt
     9241 tggccaacct gatgaacgcc tctcccctca tcacggattc cattaccgat acggtttacc
     9301 gtcaacggtt tgtgatgcac gccgcccagg tggtcaacaa ttccatagaa cggagaaaca
     9361 acgtcatgac gatcaacaaa ggcgcccggg acggcattga caaatacgcc accgtggtag
     9421 aaccgacggg gattgtcggc tttgtgaccc acctcggtat ccgttacgcc acggtaatgt
     9481 ccatccttaa ttccaattca agggtgagcg ctatggtcaa gaggacatcg acccgaggca
     9541 acctggtctg gccgggaaat tcccccctga tcatgaatgt ggaagcaatc ccaaaacatg
     9601 ccgacgtccg ggtgggggac accatcgtca ccagcagttt ttcccatttc cctgccggcc
     9661 atccggtagg cgagatcacc aaagccacca ttgagcccgg cgaaaatttt tacctcatcg
     9721 aggtccgcct ttttaacgac ctggcccgaa ccaaccatgt gtatgtagtc aacgacctcc
     9781 acaagtccga gctggacagt ctcaaccaat ttaataccca aaaatgaatt ccatcgggtt
     9841 taggcatggg ttgcggctgg ttttcctgtt ttttttccag gtactggtcc tcaaacaggt
     9901 ccatctcaac tggggcgatt tcaattacat ttcgatcatc ctttatcccc tctggatcct
     9961 tttactgccg gtgcaaaccg cccgctggat cctggtcgca ggcggtttcc tggccgggct
    10021 cggggtggat tggttttacg actccccggg ggtgcatgca agtgcagcca cctttaccgg
    10081 ctttctcagg cctttcatcc tctcctatct tgaaccccgg gggggataca accgggtgcc
    10141 tatcccaagc aaggcccacc tggggctaaa ctggtttgtg acttatgcaa gcattatgct
    10201 gatcggctat ctgttcattt acatttccat ggaagtgttt actccggcct tactcggcac
    10261 catcctggtc aaaaccctgt tcagttttat catttccatc ctcatggtcc ttctgtatat
    10321 gtttatcttt gatcccaaat gaaaggcaat gcgaggcata tcattgaggc taccgtaatc
    10381 atacttgccg ggatcctgct gtttcagtcc ttttatatcc agtttctgga caaggatatc
    10441 cgtaaaaggg cccaatatgc cgccctggaa aaagtggtga gttatccggc ccgcggggcc
    10501 atttacgacc gcaacagcaa attgctggtt tacaacaacc cggtgtacga catcgaagcc
    10561 ctttaccgca aaatcgataa aaacatggac acggcccggt tctgccagtt gctgggcatt
    10621 gaccgggcaa cttttatttc tgaactgaac aaagactggt ccaacaaaca attttcaaaa
    10681 agtaaaccgt tcccctttat caaaagggtg cccgtggatc aatttgccgg attccaggaa
    10741 atgatgcatg aatttccggg attctccgct cagctccggt atatccgcgg ctaccccagg
    10801 cccattgcgg cccatgccat gggttatatc agcgaggtgg acccggcgga aatcgaaaaa
    10861 tcaaacggag tgtacgagcg cggggactat aaaggcacca gcggcattga acgggcctat
    10921 gaaagtcagt tgaaagggga taaaggatat aaatatctcc tcaaagacaa cctcggggtc
    10981 acggtgggtt cataccagaa tggggccctg gactccgcag cgcgcagcgg ggacgaactg
    11041 atggtatccc tggatgccga cctccaggaa tacggggaac ggctgatgca gaataaaaga
    11101 ggttccgtgg ttgccattga accttcgacc ggtgaaatcc tgtgcctgat cagcgccccc
    11161 acttataatc caaatctgct gaccgtggac cgggaccggg gcaagtattt ttcagccctg
    11221 gtgcaggata ccctcaaacc gctttttgac cggaccgtcc aggctcaata tcccccggga
    11281 tccatcttca agaccgtggt agcccttgcc ggtttgcaga tgggcgtact ggaccccaat
    11341 aaggggaccc actgtggggg gtcctatacc ctcggccggg tctataaatg cagccatatt
    11401 cattacgatc cccgtaacct cggcgatgcc ctggaacaat cctgcaacgt ttacttttat
    11461 caaaagatcc aggacatcat gaacaaatat ggatcggaca aaccgggcaa aggcctggac
    11521 gagtttgccg ggtatatgcg ccagtttgga ctgggtaaaa tgctgggcat ggattatttc
    11581 aacgagctcc ccggcaccat ccccacggac gcctattata aaaggcatta ttaccgcttg
    11641 ttcagggact cgacctggaa agcggcttat gagatttcca tcgggatcgg acagggtgta
    11701 attcagctca ccaccgccca aatggccaat ctttgtgcag cgatcgcaaa ccgcggctat
    11761 tggatcaccc cgcattttta caaaggcgtg atcaaaaatc accaactgca gccccaggat
    11821 aaattcttag aaaaacacaa aatcgatatc gatccccggc attttgagac cgtcatagac
    11881 gggatggaaa gggcggtact gaccgggacc gcgcgcatag cccagctcaa aggcatagag
    11941 gtttgcggta aaacgggcac tgcccagaat ccgcacgggg cggaccattc cgtcttctgc
    12001 gcctttgctc cgaaagacaa tcccaaaatc gccatcgcgg tcatcgttga aaactccgga
    12061 ttcggctcga cctttgcggc gccggtggca tccctgctga tggagaaata catcaatgga
    12121 aaggtcgact ctaccaggac ctggctcgaa aagcgtaccc tggaacagga cctgattcac
    12181 aaaccccagc ctgcgcctcc caccaaaccc tgaattcccg gatgatcgtc tcagctattg
    12241 tggccatgaa ccaggattca gccattgggg tcaaaggcca gatcccttgg tacctgcccg
    12301 ccgaccttaa atatttcagg aaaaccacca ctccccacca tgtcattatg ggaaggcaaa
    12361 cctttgaaag tatcggtaaa ccgctgccca acaggataaa catcattctc acccgcgatc
    12421 ccttttacct gagtaccgga tgcctcattg cccattccat ccgggaggcg cttgatattt
    12481 cggcagacca tggggagaaa gaggctttca tcattggcgg cgctcagatc taccaggcgg
    12541 ccctcccctt actggacaaa ctttatatca cgagggtaag cctcgaagta cccgaggcgg
    12601 acgccttttt cccaaccatc gattataagg aatggaccct ggactcctcg gtggcttttc
    12661 cggcggatga taaaaacaaa tggccttatt gttttgaagt ctatagtaga gttagcaagt
    12721 tagcaagtta gcaagtttat cagttcaaca gtttacatct gcttcagctt attgaccttt
    12781 tcccgggcgg cctctacctt gcctgcctgg tcttcgatcg cggtttgttt gcttttctgg
    12841 tcttcctcgt tcttgagaat atttttttcc gcttctttta ttttttcgtt ggcccgctcg
    12901 atctccttgt gatagtcttc attgtccttt ttcagctttt tcatatcggc atcgagtttt
    12961 ttcatgtccc gctcgaccgc ggcgagttca tcttccgcct ggcttatttt aacttgctgt
    13021 ccgaattttt caaggagttt tccggcttct tcgtattcgc ggggataggt tcccgaactg
    13081 atataagcgg taccgagatc tatccataaa gtcacggtgg cctggttgcc ggcgctttcg
    13141 atccgggcat agatgtcgat cggcgctcca ttggcaatgc tggcgacctg ggcgtcatcg
    13201 ctgtaccatt cattggattt tttatctttt ttggttttcc ccttataggt tttaataaac
    13261 tccttgtaga catcgtccac atactcggcg gaaacatcag gaatggtcag gttgagactg
    13321 gtttgctggc ctttgctcat cactttggaa gattccatga cttgggcccg ggaggatacg
    13381 gccaatagga tcaggaaaat caaaacagaa tttaatttca taaaaatggg ttaaaatgta
    13441 ataatatcaa tctaaatttt ttcaaaatag tttcaggggg cacagatttt aagacctttt
    13501 ttaatgacgg aaatgcgggc cggaaagtta cccaacaatt ctccttccgc ttctatttta
    13561 atgatttccc cggggccggc ttccagtatc agggattcgg tgttgaaatg aaggaccctg
    13621 gggtgccgga tgattttacc ggaatagaac aaggggctca ataaaaaaac ctgaagtttg
    13681 gagaccggcc ttactgcagt cacggcgagg gccccgcttt ggggatgagc gtgcggtacc
    13741 aggtggagcc cacccccgga ataaggacaa atcccgatat tgatggtata aaaacggtcg
    13801 gcttcccggc cacccggcca actgatccgg ctgttttgaa ggcggtagga aaacaggcag
    13861 cggaaaataa accaaaggta ttgaatgcct cccgcgctca tttttttctt gcggttttca
    13921 ataaagtcca ccacgtaagc atcgtaggct gcaccggcta cattgttgaa gacctgcttt
    13981 ttttcctgcc cctgctcatc cgtgtaaacg atcaggccca ggtcatgttc gtgggtattc
    14041 ccctgtttaa tcatttccac ccattcgtga atcttaaacg ggattttcca atacctcgcc
    14101 cagtcgtttc cggtccccac cgggaacatc gtgaaggcgg tcttgttttg gatcccgctg
    14161 tccaccatcc cgttcactac ctggtgcgcc gtgccatccc cgccgaaacc aacaatcagg
    14221 cccggctctt ctttcatggc ctggcaggct aactcccggg cgtggtcttt cctggccgac
    14281 cagcggattt cgtgcggaat gccggctgat tttaagattt cggccaggcg gggccaggct
    14341 cttaaagtac gccccttgcc cgcctcggga ttgcctatga atacaagagg acccatgtcg
    14401 cacaaggtag ggagaaatga ggaacgacgg ccccggggga gtggattaca ggatactcaa
    14461 aaccggtaga caaccacccc tttcaggaaa aatggagtac ccggcgtaaa atgaagctcc
    14521 gacacggcct ggccttcgtc aaacaagcgg ctcgtggtat caaactgcgc ctggttccat
    14581 tcccggttca ggaggttttc aatggtgaac cccagttcca ttttcttaaa aacatacatg
    14641 gccgaggcat ccaggatggt ataccccttt gcccggatgg agtttgattc attggcgggc
    14701 ctgctgtcca tgagccggta ccggaggctg gcattaaatc cccgatcctg tttatattgc
    14761 agtccaccgg tgctggtgac ccttggcgcc aggggaataa aatcctctcc ttcaggggcc
    14821 ccgaccagcc tcccatggtt gacattgaag tccgcatccg caaaccattt attggccggc
    14881 tgtactctta gcccgagatc aaggcccatc ctccgggtag gcatgttggt ttcaaccacc
    14941 ccctcgtccc ccacatagac cagttcgctt tgagagtgca aggcccacaa agccatttgc
    15001 atcaggactt tgcgcccgag ctttaattca gtgccggctt cgtatcccag ggctttgggg
    15061 aggctgtttt ccgtctgacc caggactacg gaccgggcgt cgttggaatg aaatcccagg
    15121 cctgatttga tgaaaaaccg gctcctggca tcggcctggt aatagaggtt gagtttgggg
    15181 ctgagcctgc cgatgagacg ttttttaccc tcggcggccc ccgtctcttt aaatgcaaac
    15241 gaaaacagat ccagccgggt acccgcattc agggtccacc ggcggtcaat ttcaaagctg
    15301 aagtctgcat atgcccctgc attgagctgc gatactttgc cgcttaccag gggctcgagg
    15361 tcctcacgcc gcctggaatg caacaagcgc accccggtat ggtcataacg cgaggctaat
    15421 ccgaattggc tgagtaggtt ccggccccaa aagctggaat gatgttgata ggaacccaga
    15481 taacccagga tcgaccggtg atccgtctga tggatttcat ccccatagag aggatcgttc
    15541 aggtaaaaag taaaattgga aaaaaggctg aaatcgtaat cggtgtaaaa caattggtgt
    15601 ttcatcaggc cattccgggg catcgaggtg ctgaggatcg cattgaggtt cttcctggag
    15661 gtattgcccc cttcaaagga atcgatttgc ccataccttg agatttggcc ggaaaggacg
    15721 gcccgctccg gaatttgacc ggaaccgttc cattcggcgg aaaaggctga tccggataat
    15781 tgcagaaaag tccggtcgga aatccggcca tgatatttgg caaacaaatt gtaccggtgg
    15841 aatttctgcc ttttatcaaa ataagaatcc gtatagacgt attccgccgc ggcataagca
    15901 tgttgctctt tgttcggggc tgtaccgggc aagaggtcaa aaaggcccac cagccgtgcg
    15961 gtgttgaaca tgcccgcttc ggctttcacc aggctgcggt ccaggccgtt cctggtcata
    16021 aattcacccg tgccggaggt ggagaagtct ccaaaccggg tcgtgtaggg ccctttgtaa
    16081 accgaaagcc ggtcaatggt ctccgggatc acaaaatgaa aatcggcgta gccctgcccg
    16141 tgcgcatggg acaccatgtt gacaggcata ccatccaccg cgatataaaa atcggtgccg
    16201 tggtcgctgt caaaaccccg caggaagatc tgttccgcct tgccgccacc ggcatgctgg
    16261 gcgatgaata agcccgggat cagttgcagc aggtcctggg ccgaattcac cgggcgcagg
    16321 agtttatcca aagcgctgaa actgcgcggc agggccccgg cttccgaaga ttcaaccacc
    16381 acttcatgga gttcaatggt accgggctgg agttgaaaat ccaaatgctg agggccgtcc
    16441 acctggatct ccagggaaaa aaattcgtag cccagggccc ggatttcaag ggtgtatttg
    16501 cccgccggga ccgccgccag tcggaatcga ccctgttcat cgctgagggc atgactcccc
    16561 tccttttccc ggatgcggac cagggcatcg ctgatgggaa gaccgctggt tttatcggtg
    16621 attttaccaa agattccagc ttcccggttt tgagctggaa gcgaagatcc gatgattagc
    16681 aaaacatata ggcttgcaag tcgcaaaatc aggctcatcc ggcactttta aaacaggtcc
    16741 aaaattaccg gaaaagcggg atcctttttc acctgttcag ggggaagaat gggatcccag
    16801 gcattattgc gcgacaggga cttcccgggg gcaggcaagc cgatatacgc cctgcgggac
    16861 taaatccgat ttttgaatct acaagtccta cccatgcaac catttatcat tattttttcc
    16921 ctgatcagcc tttccctcta ttcccaacag agcccctcct ttattttcgg ccttcaccag
    16981 caatatctgg agacatccat cacttcaaaa aaattcaaac accgggtgct tgattcactg
    17041 attcagcagg tcgcaaagga cccgctgttt gaagtcaaaa ccgcggggca atcctttttg
    17101 ggaaaaaaca tttacctcat cagggcggga acgggcagcc ggcgggtcct tctctggagc
    17161 cagatgcacg gggacgaatc caccgctacc atggctctga tggatctgtt caattatctg
    17221 caggatgaat cccaggtcgg tcggaaactc ctgctggagc attgcaccct ctattttata
    17281 cccatgctca acccggacgg ggcggatctt tttgttcgcc gcaacgcaca gcaaatcgat
    17341 gtcaaccggg acgctctttt tttacaaagc cccgaaggac gcctcctgaa gtccatccgg
    17401 gacagcctga acccggaatt cggattcaac ctgcatgacc aaaacaaata ccttgcggcc
    17461 ggggacagca aaaggcccgc atcgatttcc ctgctcgccc ccgcctacaa cccggaaaaa
    17521 gatatcaatc cggtgcgcga gcgagccatg ctcctggccg cccagatcca cctcacgctc
    17581 caagccttca tcccccaggg catcgggcgc tacgacgatg aattcgaacc ccgggccttc
    17641 ggggacaaca ttcagaaatg gggcaccagc accgtactca tcgaaaccgg cggatatgaa
    17701 aacgacccgg aaaaacaata tttaagaaag atcaatttta ctgccatact cagtgcattg
    17761 gaatccattg ccaccaactc cttccatcat caaaacatcc gcgattactg gtccattccg
    17821 gaaaataaaa gccgcctcaa cgacctgatc atccgaaacg tcaggctgga actaaatggt
    17881 aaggaatatc tcagcgacct gtccttcaat atcccggaat cgtccggccg ggccaccctg
    17941 gtggacatgg gtgacctctc cattttcaga ggataccgcg aatgggacgg taccggtaaa
    18001 aaactggttc ccggaaaggt ctatcccagg atgatcaaaa attccaggaa gctgggcagg
    18061 ctcgaccagg tccgactgat caaggatggt tataccgaag tccggctcag gaaacccggc
    18121 cccaaaaccc aaaagcaact tctaaacgta ctgggcgcca ccgaggtaaa aaacaatacc
    18181 ctgcagcccg gggcaaaccc aaatgccttg atctatgaaa aggaggaggt catttattta
    18241 ctgagtaatg ggaacctcgt ggagatcagg ccttaatttc agcaagtccg gctatcttaa
    18301 acccgaccca atagcatgga aaaaatatcc gtctttgata tttttaaaat aggcatcggc
    18361 cccagttcca gccataccat gggcccctgg cgggctgccc tgcgatttat acacgaactg
    18421 aaggccctga accttttaga aaaggttcag ggaatccagg tggaccttta tggctccctg
    18481 gccaagaccg gccacggtca tggcaccgat acggccatcc tcctcggctt aagcggtttt
    18541 gatccggtga ccatccctga agaatccatt ccgcaggaag tggaaaggat tcaccggtcc
    18601 ggacgccttt cccttgaagg tccagccacc attgggtttg acccggggaa ggacctgata
    18661 ttccattatt tcgaaagcct gccctaccat ccgaatggcc tgcgtttcaa ggcctcggtc
    18721 caggggcttg ctccgtatac ctccacctat ttctccaccg gcggcggttt tgtgctgaaa
    18781 gaaaaggagg aagcggacag accggaagtc catcttccat accccatcca gaccagcagg
    18841 gacctggaat cctattgtcg tttgctccaa aaaccgatcg atgagatcgt actgcagaac
    18901 gaacggatgt tcaggcagga ggaagaagtg cacctgggcc tcctgaatat tttcgaaacc
    18961 atgatgcagt gcgcctataa gggttgtcat accgaagggg ttctgcccgg aggcttgaat
    19021 gtcagccgga gagcagcctc catcaataaa aaactgctcg atccggagac ctcgtattcg
    19081 gacgcccggg gatggctgga tgcggttcgc cggtcaggcc atgattttga aaccatcaac
    19141 aaatggatct cctgctttgc cctggcggta aatgaagtca atgcctcttt cggacgggtg
    19201 gtcaccgcgc ccaccaacgg ggcggcggga gtgatcccgg cggttttatt gtaccatatt
    19261 tgttttggga aaggtaagga ggacaaagac cgggacatca tccgtttttt gctgaccgcg
    19321 ggcgagatcg gcagcatctt caaaaaagga gccaccatca gcgcggctat gggcggctgc
    19381 caggcggaga tcggggtatc ctcagctatg gcggcagggg gccttgcctt atgcctgggt
    19441 ggagactaca ggcaggtatt gatggccgcg gagattgcca tggagcatca cctgggcatg
    19501 acctgcgatc cggtgggcgg actggtgcag atcccctgca tcgagcgcaa taccatgggt
    19561 gccatcaagg cgatcacagc ggcccacctg gccctcgaaa gcgatccgga acaggccaag
    19621 gtctccctgg acgaagtcat ccgtaccatg tggctgacgg ccctggatat gaacagcaag
    19681 tataaggaga cggcggaggg agggcttgcg gtaaatatcg cggtgaatgt ggcggagtgt
    19741 tgatgatgat taaggaatta aagaaattaa gaattaagaa ggcatgatcc tgtaattaaa
    19801 ggttgagcca aaaatttctt tcaaaatatt tgagcaacca aaatgttgca tttatatttg
    19861 caaccatatc gttgcattat attaaaccca ttaaataaaa aaatatgttg gcatacctta
    19921 gcctgggaag cggtttctta tccctcctta gtttattgat cctgcatttc gtcagccccg
    19981 aattcaagcc gagctggcgg atgatcagcg aatacgccct tggaaaacat aaatggctga
    20041 tcacctcctt tttcgtcttt tggagcctga gtaccttctt tctggccttt ttgttgtgga
    20101 atcaggtcgg tggtatttgg gccaggattg cgctgatcct gcttgttgtt tctggcatcg
    20161 gcgagtttat ggggggagct tttgatgtaa aacataaact ccacgggctc gctttcgcct
    20221 taggggtgcc cgccttgccg gtcgccgccc tggtcctcag ttaccagctg gtccgccagg
    20281 atgcctgggc tatccataaa tcatccattc tgtattccgc ccattccacc tggatcagcc
    20341 tggtcctgat ggctgtgacc atgatcgtaa tgatttccgg atttaaaaaa gccgggatac
    20401 ctatgggtcc ggacatcgag cctcccgatt cggttcccgc gggggtcatc gcagtgagtg
    20461 gttatgcaaa ccggatcctc gtcttttgtt atatcctgtg gctgttggtg atagcaaaaa
    20521 tatttacaac ccaataaaat aaacccggtg caaaccccgg acctccgatc ctccaaggac
    20581 ggataggatg gccggtctta ataattgagg tcatgagaaa attgattttg caaatgcaga
    20641 cttcgcttga cggatttgtt gctaccggac cgaacgatga acagtcctgg gtgacctggg
    20701 ctttggacga gatcaaaccg catgtgatcg aactgttgga ttccacggac accattttga
    20761 ttggaagaaa attggcggtt gattatatcc ctttttggca ggacacctat accaaaccag
    20821 aagacccgat gtttgaattt gcaaaaagaa ttgtggctgc gaaaaaaata gtattttcca
    20881 ggaccctgga acaatccatc tggcccaata cagaactcgc aaagggccca ttggctgagg
    20941 aagtcaatgc actgaaaaat caagatggaa aggacctcgt ggtctacgga ggcgcttctt
    21001 ttgtctcggc cctgatcaaa gaaggactga ttgacgagta tcaattttat atcaatccgg
    21061 tggtcctggg gaagggagta cccgtttttg atctaatcga ctcctttcga ccccttgttt
    21121 tgatcaaatc gatggttttt cccggcggta ttattttgac caaataccaa ccaaaatagg
    21181 gacatgccat gaagtcgttc tgcaggttca ggttcatgca ccggcagggg gatatttaag
    21241 ctgatgtttt tatcttcgtt tgacgccgac caggcgatac aaagggtgaa gatgcaggca
    21301 atagaccaat atcggcgggc atgaataagg gactgagcag caaagaagtc gcggacagaa
    21361 ttgcccgttt tggatataat gaacttccca cttccaagtc aaaaaacatt ctgcatatcg
    21421 ctcttgaagt catcagggag cctatgttta ttttactgat aagttgtggc tccctctata
    21481 tcattctggg agactatact gaaggcatca ttctggtttg ttgggttttt attatcattt
    21541 tcatcacctt ctaccagcat cagaaaacag aaagtgctct cgatgcactc aaaaaacttt
    21601 cttctcccag ggctttggtc atccgggatg gtaaagaggt ccggattgca ggcagggagg
    21661 tagtaccgga tgatgtggtc atcttaaatg aaggtgaccg ggtacctgcg gatgggatct
    21721 tatccgaagg tttaaatctg actatcgacg aatccctgct taccggtgaa tcactgcctg
    21781 tcaccaagac cactcataac gaaaaagaac caaacatccc ggtatacagc ggcaccctgg
    21841 tcgtccaggg aaagggcttg atggaagtaa agactacagg gagtaataca gaatttggaa
    21901 aaataggcaa atccattcaa catatcgaac aggatcaaac ccggttgcaa aaggagatga
    21961 aatcattgat ccggaatctc tttatcgccg gagccctgct aagcgttgtg gtgatcgccg
    22021 gtttttactt cacccggggc aattttgtgc agtctttgct caatgggctg gctacagcca
    22081 tggccatgtt gccggaagaa ttcctggtag tgctcaccgt ttttcttgcc ctgggcgcct
    22141 ggcgcttatc ccaaaaaaat gtactgaccc gcaagccttc ggcgatagag accctgggtt
    22201 cggccaccgt gttatgcagt gataaaacag gcaccatcac tcaaaacaaa atggaattgg
    22261 ccagcctgtt tgcgaataat aaaatgttca ataaatcatc tttcagggaa aaggctcctg
    22321 aaatccggga attattggtc catgcatttc acgcatcgca aaaagacacc attgacccca
    22381 tggaaaaagc aataggaaaa ggatataatg aatgggttat cccaaaagcc ctgttgccgg
    22441 atctggtaaa agaatatcca ctgagtaccc gtttattcgc catgaccagg gtattgaaac
    22501 caaagcacga ggaccactac ctggttagct gtaaagggac ccccgaagct gttttttctc
    22561 tgtgcaaaat gaatgatacc gaggtatcgg aacatttaac cacggttcaa aaaatggcgg
    22621 aacaaggcca aagagtctta ggagtggcaa aagccgcatg gaataaaaaa gaaataccgg
    22681 acacccagca agcatacgaa tttgaattta tcggattaat aggctttgaa gatccgatcc
    22741 ggccagaagt gccccgggcc attcaggaat gtcatgaagc aggcatcaag gtaattatga
    22801 tcaccgggga ctatccatcc accgccaaat gcattgcttc acaggcagga atgaacaccg
    22861 atggggttgt attgactgga acagacttga aaaatatgag cgagtccgaa ctggctgaaa
    22921 aaataaaatc ggtcaacatt tttgcgagga tcgttcctga acaaaaattg cagataatca
    22981 atgcgttaaa aacaaatggc gaaattgtcg ccatgaccgg ggatggggtg aatgatgctc
    23041 ctgcgttgaa agcggctgat attggaatcg ctttgggcgg aaaaggcaca gatgtagcaa
    23101 gggaggcgtc gtcccttgtt ttgttggatg accactttag ctcgatcgta gttgccattc
    23161 gctccgggcg taaaatattc gacaacctgc aaaaatcaat gtcctatatc attgccatac
    23221 atatacctat cattggactc accctgctac cggccttttt cccgggactc cccatccttt
    23281 taatgccgct gcatattgta tttctggagc tgatcctgga tccggtctgt tctatagctt
    23341 ttgaatccga aaaggaagag aaggacatca tgaagcatcc tccccgcgat ccggaggagc
    23401 ccttttttgg attcggaaaa atagttggca gcgcgtccgt tggcctgtta ttgatggcca
    23461 tggtcttggc catttacttt ttatccctgc aagaaaggca taccgatgga gaaacccggg
    23521 ccatcgcttt ttcatcgctg atcctcggaa acatttttct catccttacg accatttcca
    23581 aaacacggaa ttacatcgac gtactgctgg aaaaaaatat ggccttgctc gtaatcctgg
    23641 ttattgctac tgcactcatg attatggtaa tctaccatcc ttacctcaga acggtcttca
    23701 gctttgatta cccgggattc agccatttta acctcgccat tgcaggtgct ctggcagtgc
    23761 tgtttatcct cgaaaccata aagatgatta aacagaattt tataatgagg acccccgttt
    23821 aacaatggtc agcaggctga atttatttaa ttatttttaa ttatttaatt atcaatgaaa
    23881 cggtttttat tccttaccct gtttaatgcc ttatgcgtac tttttgctta tgcccagggc
    23941 atttcaaacg aggacatccc tgataaaaag gaaatttatt ccctgatcga tcagtattcc
    24001 tctgcacggg agaagcgtga taccgccatg ctcagacatc tcttaacacc tgacgcggac
    24061 cagttggttt cttcgggtca atggcgggtg ggtatcgggg aagcagtgaa gggcatgttg
    24121 ttatcttcag aaagcaaccc cggctcgcga agactcatcg tagacaaaat caggtttctc
    24181 gattcaaagc atgccatcgc agatgcccgg tatgaaatac agaatccgga cggcgcggcc
    24241 aggaagatgt ggagtacctt tattgtggtg tttcaaaacg gaagttggaa aatagcggcc
    24301 atccggaata tgttgccggc cccagcccga taggcctgca taccgacatg cacccctctt
    24361 gttccgggat gaaaaatttc ttattttagg acttccttca taaaatataa gactgagcta
    24421 aatcaaaatt gaatcccagg gattcattat taatcttatt gatcgaacat gaataagctc
    24481 gttttctttt tctgccttat cccctccggt atttttggac aagctgccaa tcctttaaaa
    24541 gggtatcccg gaatccggga tggagtattc gtttattttc ccccttcccg ggccatgctt
    24601 ctgttgggag gcggcaaaag cagcccgaac tcctcggaga gcaatgtttg gaaatggaag
    24661 gacagtcaat gggctatgat tccagcttcc ggtccgggat cacgggattt ttttccatgt
    24721 gcctatcatc ccggttcagg caatatctat tgttacgggg agcaggattc tccgggaggg
    24781 gtcttttggt cctttgacgg cgcccaatgg aaatcaacga aaataaccgg actggaatcg
    24841 agggaccacc accagatggt atatgccgac catttaaacg cctttatcat atatggagga
    24901 gccaatgaaa ggggtattct cgatacgctg acctggctgg tccgggacgg aaaagccact
    24961 gccttgaata ttcccggacc cggtatccgt tatcattccg caatgacata cgataaacaa
    25021 agaaaaaagg tggtgttgta cggtggaggt aagaaaccct gggaacactg ggaatttgat
    25081 ggattgcggt gggtaaaaat caacagcagg acaagccccg gtaataaatt ctatcatttc
    25141 atggcatatg atgaaagctc agataagatc attctgcacg gaggatggag aaaccaggac
    25201 ccctttgatc cggtgaataa acagaaccct cagacctggg cctgggatgg gcaggactgg
    25261 acgaatattg ccggggaatc catattctct attgcgtttg gatatgacgc aggaaggaaa
    25321 caagtggtca cctatggccg aagcgggggg ccggataatg cgcctttggc cttatggatt
    25381 cttgagaatg gaagctggaa aaaaatctcg gactatggta tgccgaatac gaatgactga
    25441 tttttcaacc cccttttcac cttatatgcg taatttcata ttcacatcct ttcatccatc
    25501 gatctatgaa attaggcgct ttttccgtaa gcctcagcgt aagggacctc agggcctcca
    25561 gggaatttta tgaaaagctg ggtttttctg tcttcgccgg cgaccccgga aaaaactacc
    25621 tcatcatgaa aaacggcaat gcgctgatcg ggcttttcca tggaatgttc gagaaaaaca
    25681 tactgacctt caacccaggt tgggatgaaa acgcccatcc cctggacgaa tttgacgacg
    25741 tgcgcaagat ccagcgtgag cttaaaaata ataatataga cattatccag gcggcggacg
    25801 aggaaaccgt cggcccggca agttttatgg tggtcgatcc ggacggaaac cccatactgt
    25861 ttgaccagca ccgctaaacg aaggggatca ggacaagggc aaactcagga accaattccc
    25921 gaagcgctcc caaaagccga agcggtaaaa actaagaacg gaaaaatgat aacccaccag
    25981 gagggccagg accttcggga agacatccag cctctgatgg gtcttccaat cccagtacac
    26041 caaaaaaagt aaaaggaggt ctgccagcag gaatccagcc accggggcaa tgggcctgcc
    26101 ttccaaaacc ggcaggtaat tcagcagggg cctgaaatgg gcatagatca ggcggtccgt
    26161 caccggagta aacaggggga acaccgtagc cagcatatac cgcgcatgca gcaggggcct
    26221 tcgccggtga tagatggcca ggccgtataa aatagccagg acaatcgtgg cgttgaccat
    26281 cagcgctaag gaataaaggg atagtggatt ccatcccccc cggcctgaaa gctggtggtg
    26341 gatcaggttc agagtcagga tcaacagcac gggcatcagg atataagata agcgtcccag
    26401 gcgccggtgc aggacgaacc gctttttccg gatcagccag gcctggccta tgagcatcat
    26461 gcaccaaagg gtcatggcaa tcccatgggt atgaaaacgg acctcctcct gatctcccag
    26521 gtgtgagtaa taacttggcc agaaggccca taaggcgaaa gcaaaaaata aaacaaaagc
    26581 caccacgctg tagtcgtata gaaaccggtt tgtcttcatc aattacctat cctttttcgc
    26641 gccgaaagat aagcccggtt ccggagttac tacttgtgtt ttcccgggag atggtgggct
    26701 gatccaggga gctgtctttg gagtttcaaa cagccttggg ctaatgtttg cgcacggagg
    26761 gcacggggcg cacggaggaa aagaatgggg cattatgatg agaaaagcag agcggccatc
    26821 tttacggtgg cggaaacgaa aataaatccc aatcaaaata tcaatcactc cgtggtctct
    26881 gtgccctccg tgcgaaattc cccatgagca atcccccgtg cgatattaac ccctaaattt
    26941 gccctcccaa tgaaaaaacc actcctcatc cagaatcaga aatttgaaaa aaccggccaa
    27001 gcctccgtgg cccttgaaac cggggaatat cgggaatgca cctttaacca atgcgatttt
    27061 tccaatgcag atttcacgga ctgcatcttt tcagactgcg aatttaccgg ttgcaatgca
    27121 agcctgtgca aattgacccg gaccggattc cgggaggtcc ggtttaaaaa ctgcaaactc
    27181 ctgggcttac attgggagga ttgcaacagc ctgatctaca acctgacgtt tgaccattgc
    27241 accctggatt tttcctcttt ttataatctg aacctgagag aaaccgtttt ccgacattcc
    27301 agtcttaaag aatcggattt cacggatgcg gatctcagcc gcgcggtctt tgaatcctgt
    27361 gacctggacc ggaccatatt ttatcatacc aacctggaga aagccgattt ccgctcggcg
    27421 cgttttttta ccatcgaccc cgagcagaac cggttgcggg gtgcgaaatt ttcggcgggc
    27481 ggcttgcctg gtcttttgaa aaaatacggc ttggaaatcg atggcgaata aaaggaaagc
    27541 aattccttac atttatatcc atgctgatgg aaatgcattt ccctgccccg ccgctgggcc
    27601 agtttatcga aagtttctta tactataaag actatcagcc cggccaccta atcgaccggt
    27661 ttttcccgga cggaaacatt tatgccgtca tcgatctcaa cgaagaaccc caatatattt
    27721 ttgacaacca gaccctccgg cccattcaga cctgtcgtaa agtatggttt tccggattga
    27781 ggaccaatta tatatccatt ccttcctgcg gagaaagcga actgtttgtg atcaatttca
    27841 ggaaaggccg ggcctttccc tttgtaaaag gtcccctcgg tgaatacacc gatgctgtag
    27901 tggatggcgg gcaatctttc cggaaggaca tcctgcgggt ccgtgaagcc ctattgcccc
    27961 ttccgggg
//
LOCUS       DAOQXT010000062        27609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2009_length_27609_cov_6.675728, whole genome shotgun sequence.
ACCESSION   DAOQXT010000062 DAOQXT010000000
VERSION     DAOQXT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 27609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27609
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2009_length_27609_cov_6.675728"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..965)
                     /locus_tag="PKM27_11585"
     CDS             complement(33..965)
                     /locus_tag="PKM27_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR07949.1"
                     /translation="MEMKPAGLLPSPVDQVPWGGEVNWLVKRDDLIHPLVSGNKYRKL
                     KYILHDFHRGNHRGLISFGGAFSNHIHALAALCGMNGIPSVGIIRGEDDQANPTLAFA
                     RNQGMVLHFVSREAYRLKLESERMEAILDRYGDFYLVPEGGDHPLAERGVGEIVHELE
                     SLGLKPDYLAVSAGTGSTATGLIKTLVNKGWTTRVLVLSSLKAPGLRDKIAIGSGIGR
                     NEFDYLDYSLGGYARTNPLYLDFLRQFFQKTGIPVDPVYNGKVVFGLDDLQKRGFFKA
                     GASVLWLHTGGLQGLEAYHYMQRKKFGSKGNYLK"
     gene            complement(956..1513)
                     /locus_tag="PKM27_11590"
     CDS             complement(956..1513)
                     /locus_tag="PKM27_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07950.1"
                     /translation="MMVFTGCREAASIPKPHAFPRIEFPVGKSRLVDEAYCPLRFEFP
                     DYGRVVQDTGFFDERPSDPCWFTIEISQLNASLHCSYGRILKKSDFDKYIEDEFKLLG
                     KHNIKADFRDEVELKNKQGVGGMAFLLEGPVATPYQFFLTDSTRHFFRASLYFNAQVN
                     PDSTRPVLEFLKKDLRIMQETFAWK"
     gene            complement(1575..2024)
                     /locus_tag="PKM27_11595"
     CDS             complement(1575..2024)
                     /locus_tag="PKM27_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNR07951.1"
                     /translation="MINKVTLVGNLGKDPEIRHLEGGTSVAKFSLATNENYRDKNGEW
                     QTQTEWHDIVAWRQLAERVARDLHKGSLVYVEGKITHRKWQDKEGQDRYSTEIVANTI
                     RSLDRRETSGGNRSYGENIPGAESTPATRNDYTSKDVGLPEDDDLPF"
     gene            complement(2337..2696)
                     /locus_tag="PKM27_11600"
     CDS             complement(2337..2696)
                     /locus_tag="PKM27_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNR07952.1"
                     /translation="MERKINKVTLRGYVANEPRVRWTEQGSKYVIFILATWGSPCRME
                     DSKTSFIQWLKVIAWGQVASSVQRVLYKGCQISLEGRFYWGGKRLGDGMLQARSEIVL
                     DQFELEIRHWGKTGIRA"
     gene            complement(2788..3162)
                     /locus_tag="PKM27_11605"
     CDS             complement(2788..3162)
                     /locus_tag="PKM27_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNR07953.1"
                     /translation="MTHEANKVQLTGFLGKEPQTKQFESGSKNASFTLAIHDNYAGKQ
                     GKKVEQTQWHQVVAWGELAEQVEKTLHKGNKIALEGRLNSRNYTAKDGTTRYITEIIL
                     SRFEVLPKTERLEVTASASTEI"
     gene            3537..4349
                     /locus_tag="PKM27_11610"
     CDS             3537..4349
                     /locus_tag="PKM27_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07954.1"
                     /translation="MTRLIQSVKSLAFILTLGFVLAGGFLHADAPDWITEKYPAYTIH
                     YTLQDECNKLEYLSLIDEGIKSVESFFNSPFQKPFEVIIHPDRHSLETAWQKQLNLPE
                     FKSECWMVASGDAYRLDMISPLLWERESCEHRFADKDKTRKLIIHELVHVFHGQRNIS
                     PDFSELSGMDWFVEGMAAYVSGQCDEDRLAQVRTALEQHKLPEDLENFWTGSFKYGLS
                     GSMIQYLQTIYGKQRLADLLKINSLKDLLAELDTTESVLLFGWTQFMNGPGH"
     gene            4541..6703
                     /locus_tag="PKM27_11615"
     CDS             4541..6703
                     /locus_tag="PKM27_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR07955.1"
                     /translation="MVIAPKASSTNYYISSSDIRANDSNPGTSPLAPWKSFSPVNSKA
                     SLATGDTIFLKRGDTFREQMNLKAGRNLVITAYGTGELPVISGADQVASWTGEDGIYQ
                     VKFSKQALNFFVNHKEQVLARFPDEGSYLTVDQATNDYLQDAALGSMDLATLNQSQVC
                     VHTAQWCWEKSGIVSASGNKINYLHPTLRVPTAGYGYFLYGHLSYLNTGKEWVYDSIN
                     QLLYYKPVSGNPNTMTCEVTVRAYGILPSKSVTGLHISQIAFAKQGESGVALPYPENE
                     NFKIDQCRFEGQYQHGVDIQGRYAEISNCTFREVDGLALFIHGTGFGARIHHNTFRNI
                     GPFRNSGIGQEINLSAIKGAFVDSCHIHHNDIDSTGYCGIAMDGKYNLIERNIIRNAM
                     LINNDGAALKSFGSGSQYNIYRNNFISLSDGNPEGNPPGTHFSTPAIYLDFSVSRCTI
                     QENTIYDRTQRGIFLNSGTHDNTVTGNVVYGANYCLDFNGNPILPTPMTGMTVKNNYF
                     MVKTSGDYLIRMLDNTGGYHQGEIDYNCYFQPYNPSLFAFLPPSTSLAFSTWKSTTGF
                     DQHSKSNTVSWNPSKPTDTLFINPSDNPLTIKLGPNKYTDLSGEDLCGSFTLQPYTSR
                     ILIKSSTLCIPTRMNGKPRSGPEVFPNPATSFIQIRSEEAIKELVLTDLNGRQVLKVS
                     HPSAETPIGLPAGLGQGMYLLQFSSVSGTTIKKIHIVR"
     gene            complement(6771..7070)
                     /locus_tag="PKM27_11620"
     CDS             complement(6771..7070)
                     /locus_tag="PKM27_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07956.1"
                     /translation="MKLYDIAHSRAGDKGDTLTLSLIPYEEKDFDWLCKTVTVEKVKE
                     HLKDTVRGEIIRYALPNISSLLFVCHHALSGGVTTSLGLDAHGKTMSSALLEMEI"
     gene            complement(7067..8389)
                     /locus_tag="PKM27_11625"
     CDS             complement(7067..8389)
                     /locus_tag="PKM27_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HNR07957.1"
                     /translation="MKDKIRIGCGAGFSGDRLEPAEVLVKEGRLDYLVLECLAERTIA
                     LAQKAKMKDPSSGYDPLLERRIRSLLPLIPDKKVRLITNMGAANPLAAADRIIQIAKE
                     TNTRIQVAVVTGDDVLDHLEPGLLTLETGQPLKAIGKLISANAYMGTEGILEALQTEA
                     SIILTGRVADPSLFLAPMMHEFLWRADDFSLLGKGTVTGHLLECAGQLTGGYFADPLK
                     KPVPGMAKLGHPFADVYPDGKTVLGKVPGTGGMLNLQTVKEQLLYEVVNPHEYYTPDV
                     IADFTSVRLKQLGPDRVEVMGGSGKSKPSSLKVSVGYPAFYLGEGEITYAGENAAGRA
                     RLAAEIVSERLSGLVQNLRVDLIGLGSVHGTAFRHAAEPYEIRLRIAGKTETQDLARR
                     IGEEVEALYTNGPAGGGGVRKNINEVIGIVSVLMPRDQIVPKVELKST"
     gene            complement(8386..9681)
                     /locus_tag="PKM27_11630"
     CDS             complement(8386..9681)
                     /locus_tag="PKM27_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015137 - citrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015746 - citrate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate:proton symporter"
                     /protein_id="HNR07958.1"
                     /translation="MLAILGFSTILVFLVLIFTRKMSVITALVMVPLLAALVAGFSVK
                     ELSGMMLTGIQQVAPTGILLIFAILYFAVMLDAGLFDPVINRIVRQVKGDPLKVVLGT
                     AVLTMLVHLDGDGTATFMIVLSAFLPIYKELKINRLVLAGVVALSVGPLHLVPWSGTS
                     ARAITVLNTDAVHLFNPNIPAIIAGMAWVALVAFYFGKKERARLGILPLNYDLKSNLN
                     EEQRSLRRPGLILVNALMTVLLIITLMSGKVAAPVLFMVAGLLALWINYPRLGDQHKV
                     LKSHGANIFMVSSMIFAAGIFSGILTGSKMIDAMAGSLVDILPLEYSRFLPLLTALTS
                     MPASLVFTPDAYYFGVVPVLSSAAEQYQMDPLEIGRAALLGQMTVGFPLSPLTASTFL
                     LTGLCEVDLADHQKFIFKWAFGTTAIMTLMALLTGSIHL"
     gene            9734..10672
                     /locus_tag="PKM27_11635"
     CDS             9734..10672
                     /locus_tag="PKM27_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR07959.1"
                     /translation="MRNAYLPSMEVIGTRGLSKSFGLNRALDQISLSIGSGEIYGFLG
                     LNGAGKTTLIRSLLGMINPDQGQIHLFGRELNPKFDQWNKIGYLVESAKAYPELTVAE
                     NLKVFGLLRRIKVPALEADAIERLKLAPYRNTKSKALSLGNLQRLGLAKALFHKPALL
                     ILDEPTNGLDPEGIVEIRNLLLDMARTGTTIFISSHILAEISRLADRIGIIHKGKLVR
                     ELHAHELNRQRTTSLRLRTRDNPKAREALLNQGFPAIQIHPSWIEIKDERAIQNPSSL
                     TRWLSLQDLPPEEIYIFKEDLEHFFLRTLQIENQDD"
     gene            10665..11444
                     /locus_tag="PKM27_11640"
     CDS             10665..11444
                     /locus_tag="PKM27_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR07960.1"
                     /translation="MTDFSHAVRAEWLKLKGSPMVRFSFIAFGLAPFMVGLFIIFIQY
                     PELAVKASSFSAKAQVMNIQPNWPSFLAVLQQALAVGGILVFGFVASWIFGREFSDGT
                     LKDLLALPIRRSTLVQAKFAIYFIWCLGLSFSNLVIGTGIGILLHLKDFSAGPQSLTN
                     YVITTFMVILSGSPVAFIASWGKGYLAPLAFVALTLVLSQILAAAGFGQFFPWAVPGL
                     FSDISGGTNLLTGWSHVILCLTGITGYFLTRIYWTKADHIS"
     gene            11573..12949
                     /locus_tag="PKM27_11645"
     CDS             11573..12949
                     /locus_tag="PKM27_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNR07961.1"
                     /translation="MITVIIPALNEEDHIGSVVRFARSQPLVNEVIVVDDKSSDRTVA
                     IAQENGAKVITSTKLGKGASMKEGILFATNEILTFLDGDIDPYPFYTIKLLTEPLLKG
                     EAEFVKSAFSRNAGRVTELVAKPLLSIFFPDLLAFSQPLSGMIAGKKSLFEKLDFRDD
                     YGVDIGILIDMHLMQVRMKEVEIGYLENKSKPWQALGKMSKEVAQTIIFKAASSKNPN
                     YNVEELGVLNEIRSQMEFALVDHLSNLSKLMAFDMDNTLLRGRFIDACANRFDFKKPL
                     MDIRSSEKDETVLTKRIANLLKGRTIQELIETVEQIELVEGTKEIVTELKKRGYIVGI
                     ITDSYDCIANHIKNKLGMDFTLANELEFSKGICTGEVKIPSFLYNHPKSLCRHTLCKT
                     NALISILEKYDIKMENTIAIGDSNNDLCMIKEAGMGISFCSREELLNHHADLVISEPS
                     FAELLEVT"
     gene            13065..13643
                     /locus_tag="PKM27_11650"
     CDS             13065..13643
                     /locus_tag="PKM27_11650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNR07962.1"
                     /translation="MPNILILSSSVRLGRNSHRLALFFRQYLLENQLAQAEILDLNEY
                     QFPIFEERLMYQTNPDPRVLEFAEKIKAADGVIIVTPEYNGGYPASLKNAIDVLEPEW
                     YKKPVALVTASDGQFGGSQVITALLLTFWKLGAVVVPARYPVPKVQEAYGPDGSPTDP
                     ATTNKFAKKFIKELLWTVKAVKAGMKETSPGA"
     gene            13731..15089
                     /locus_tag="PKM27_11655"
     CDS             13731..15089
                     /locus_tag="PKM27_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR07963.1"
                     /translation="MKRRLTSEDYLRELEEFRRFPDMNPGPMIRTNLEGTVLSSNVAA
                     QNLFGKDFNGKNWKQICPGITTEVWNKILQTEYAFPLEAQFSTLSYIFNHRVDQLGRM
                     VFVFGTDITVQRSAEKQMKQDKKMATLGTLAAGIAHELNNPAAATRRASQQLKETLHK
                     IDLNRQNLYTVRLGPGETDLMLELAQKASEFTKQRSSLTALQISDKETETEEWLEQLG
                     FENAWEIAPALVAIGIEKQFIDSRAKSLSPDALKAILQWVGLLVPVYQLLHEIYEASS
                     RISDIVVALKNYSFLGQAPVQAVNIHEGIDNTLLILKHKLKAGITVQKRYCEDLPPVT
                     AYGSELNQVWTNILDNAADALKGNGEIIIRTYKLDNMATVEIEDNGPGIPETILPRIF
                     DPFFTTKEPGKGTGLGLSTSYLIITEKHKGHLRARSRPGSTVFTVQLPFNQETNTEPP
                     QG"
     gene            15109..16770
                     /locus_tag="PKM27_11660"
     CDS             15109..16770
                     /locus_tag="PKM27_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR07964.1"
                     /translation="MSKPVIFTVDDDLEVLAAIHRDLRQHYKNTYRILKANTGNEVLE
                     AARELKQRSVPVALFLVDQRMPDMTGTELLKELVKWYPEACKVLLTAYADTEAAISSI
                     NEIGLDHYLLKPWDPPEQKLFPVLDDLLSKWNTRVKLPYEGIRVVGSRWSARSYQSKE
                     FLSLNQVPYQWLDIEEDGQTRELILPLSNKLKKLPVILFPDGTYLVEPTNLELAEKVG
                     IQTRAQQQFYDLVVIGSGPAGLANAVYGASEGLRTLLIERNAPGGQAATSSRIENYLG
                     FPAGITGADLAQRAVAQAKRFGAEMLTAQEAVSFRREDPYRYVVLADGSEICCYAIVF
                     ATGMSVRKLEVPGIERFQGIGVYYGAAMSEAAIYKGKNIGIVGGANSAGQGAVFFSRY
                     AQSVTMLIRAESLRPGMSEYLVDRIEDSPNIRVIPEVEVIAVDGDQALEKVTIRNIKT
                     GEEKLLDLAALFIFIGSQPHSEMAAGFVARDAKGFILTGPDLPRTGNKVPGWTLDRDP
                     FLFETNVPGVFAVGDVRSGANRRVAAAVGEGSASIYMVHRYLTTV"
     gene            16791..17384
                     /locus_tag="PKM27_11665"
     CDS             16791..17384
                     /locus_tag="PKM27_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNR07965.1"
                     /translation="MSALLKTRVEQTLGQAIPLTEFNAFLDLLYSKSFDKKSVLAEEG
                     KVCKNIYFFQKGSAYSYILDTKAEKHAIQFALAGYWISDLYSFFSGRPGKYNIETLEP
                     SEVLVLNKQQFEAACDTIPVFNRFFRILIQNAYVSLQFRLARTNSEDAEFRYREFSSL
                     HPDFVQNIPQYLIASYLGVKPQSLSRIRKALLKSSAK"
     gene            17580..18842
                     /locus_tag="PKM27_11670"
     CDS             17580..18842
                     /locus_tag="PKM27_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR07966.1"
                     /translation="MSFQSLQIVKPILKALEEEGYTTPTPIQAQAIPIVLQGTDLLGC
                     AQTGTGKTAAFAIPILQILGAAPHTPNKKRKIRSLIVTPTRELAIQILESFRAYGRHT
                     GLNFTVVFGGVNQNPQTSALRAGVDILIATPGRLLDLMQQGFVSLQDIEIFVLDEADR
                     MLDMGFINDVKKIIARLPQKRQSLFFSATMPPEITKLAGTILHNPSRVSVTPVSSTVE
                     IIEQFVFYVEKGNKNALLVELLRDKKIKTALVFTRTKHGADKVVRMLHQHRIPAAAIH
                     GNKSQNIRQRALSEFKAQRTRVLVATDIAARGIDIEDLEYVINYDIPNISETYVHRIG
                     RTGRAGAGGTAFSFCDAEETAFLKDIEKLIGKKIKVIENHPYRMQFTGATLPKNLPRS
                     KRSSGKSRNSPVTGGRISHYRSPLKASW"
     gene            19018..19551
                     /locus_tag="PKM27_11675"
     CDS             19018..19551
                     /locus_tag="PKM27_11675"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNR07967.1"
                     /translation="MKKLTKWVLDPAHSELQFKVKHMMITNVNGEFRNFDASVETVDD
                     DFTKGRMHVVIDATSVFTNNADRDNHLKSPDFFDAEQFQNLEFEGTSIKKLDEENYQL
                     KGLLTIKGIQKPVVLDMEYGGIQKDPWGNEKAGFTLTGKINRKEWGLNWNAALEAGGV
                     LVSEDVKINAEVQLKRA"
     gene            19632..20384
                     /locus_tag="PKM27_11680"
     CDS             19632..20384
                     /locus_tag="PKM27_11680"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNR07968.1"
                     /translation="MKILENKVAIVTGAGSGIGRAIALLYAAEGAKVVVSDIAEKGGL
                     ETVSQIKAAGGQAIFVKADTSSPEDNKNLVDQTVKEYGGLHIAVNNAGIGGPLGPTGD
                     YPIDGWKKVIDINLSGVFYGMHYQIQAMLASGGGSIINMASILGMTGTKGSPAYVAAK
                     HGVVGLTKAAALEYATSNIRVNSIGPGYILTPLVTNALDEATLKALVGLHPVGRLGKS
                     EEVAELALFLGSDKASFITGSYYNVDGGYLAQ"
     gene            20669..21601
                     /locus_tag="PKM27_11685"
     CDS             20669..21601
                     /locus_tag="PKM27_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNR07969.1"
                     /translation="MKNKFRRIPIPRFPVQRFLILLASCILPWMSKAQPVQKNYKLAM
                     VQMKVEAGKPEVNLARATERIAEAARNGAVIALLPEAMDLGWTDPSAKTKAYAIPSGP
                     TCQSLIRAAVENKIYVCAGIIEKDEERTYNAAVLIDPTGQVLLKHRKINELDIAHDLY
                     ATGEGLQVAHTPIGTLGLYICADATAKGNTLSNSLAYMGADMIISPCAWAVPPGFDNQ
                     KTPYGDTWRKVYRPVSAQFDLWVVGVSNVGKIEAGPWKDWDCIGSSLVYAPGGIEVLQ
                     APFGAEADTIIYVDIALKDRPARGTTWNTYWQNK"
     gene            21634..22170
                     /locus_tag="PKM27_11690"
     CDS             21634..22170
                     /locus_tag="PKM27_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR07970.1"
                     /translation="MKSSQDVFSVRNALPSEFAEIGLLMVEAYSQLEGFPKETEQPHY
                     YQMLANVGDLTAKPGVELLVAVSGEGEPAGAVVYFADMLYYGSGGSATKAKNAAGFRL
                     LAVSPKFRGRGLGKLLTIACIQKTIKTGLGQLIIHTTRAMQTAWKMYESLGFQRSEDL
                     DFLQGDLPVFGFRLRLKE"
     gene            22206..22643
                     /locus_tag="PKM27_11695"
     CDS             22206..22643
                     /locus_tag="PKM27_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNR07971.1"
                     /translation="MKQIETQIIIHTRPEKVWAVLMDFSSFPDWNPFIRLIGGGHQAG
                     ESLRLTLKVPDGMEMTIGPQILKAEPNKEFRWKGKLIISGLFDGEHYFKLIDLGDGRT
                     QFIHGEQFSGILVGLFGGTVAKTKIGFEKMNQALKKRCEEIKN"
     gene            complement(22695..24059)
                     /locus_tag="PKM27_11700"
     CDS             complement(22695..24059)
                     /locus_tag="PKM27_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNR07972.1"
                     /translation="MKNIVIIGAGLSGTLLTINLIEQGGSGGPVHITLIDRNPERDLG
                     PAYSSREEALLLNVTAGRMGAYSSAPGHFLQWVRKKGLSAGEWDFLPRLLYREYIQEL
                     YQQAMKKKAKGTAMTRIQGEVTDISIRNGKAEVFTENHAFHADQVVLALGNFPPRHLL
                     LANRSFIQSRHYFQNPWPDQVLDSISKDETVVLIGTGQTTVDLLVRLNRIRHEGKMIA
                     VSRHGLFPLAHSTFKEYPSFREEIMKQNSIAGILRVVRLHIKKSKELGQDANAVMDSM
                     RPYTQELWMNLTSEEKARFLRHVFRYFEILRSRIPPESEEVVKRLQESGQLTVVRGRI
                     TDLVPQDHHLEMIYIPAGKSRPETIASPRVINCMGPETDYFKVDHPLIKNLLNKAELQ
                     PDPFHLGMNALPNGAILHSDGTAHNILYTLGFPLRGIIWETLAAPEIRAQAEQLAHRL
                     LQKP"
     gene            complement(24087..24491)
                     /locus_tag="PKM27_11705"
     CDS             complement(24087..24491)
                     /locus_tag="PKM27_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07973.1"
                     /translation="MKQLKAALEKKYREDYYTGQLYVGEGTITYFPFTPLDLKKLKLK
                     IALVYVYPDDRLEIWLAGQNKNIQKAYWELFRGSDWNKHFIPPTIRDRFSIVESVLAE
                     HPDLDRFEELAVKAEQKALGFIRDVQEVLSTG"
     gene            complement(24609..25052)
                     /locus_tag="PKM27_11710"
     CDS             complement(24609..25052)
                     /locus_tag="PKM27_11710"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNR07974.1"
                     /translation="MKKVESSILIENYPAKTLEAFTSEHMLKDWWGVDRSLIKLGVGG
                     LYTLAWSADGNGFSFVMTGLVREYDPEHVLRIGDLVYLNPGKPLFGPMSLTMRAKDLG
                     GTTELYLCQDGYRDGEDWDWYYQAVREAWPKVLVTLKQYLEKTGF"
     gene            complement(25114..25998)
                     /locus_tag="PKM27_11715"
     CDS             complement(25114..25998)
                     /locus_tag="PKM27_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR07975.1"
                     /translation="MPLLSAFPKAFMQDLCKTGDMKLSEWIDLACALDIDGLEWYAGF
                     LEMADEKNWTTFRKRVEDQGKVIPMMCCSPDFTHPDPEFRAREITKEKRWIDMTYALG
                     GSYCRVLSGQRRPELSTEQGISLAAECIEACLPYARERGITLILENHYKDDFWEFPEF
                     AQKMEVFCKLVDRVRHPNFGVNYDPSNTYLAGEDPLELLYRVSGRVVTMHASDRFLKE
                     GTLEDLRKEEGGAVGYARRLSHGEIGKGLNDYDAIFSELKRVGFDGWISIEDGVEGME
                     QLERSVAFLREKIYKYWP"
     gene            complement(26097..26888)
                     /locus_tag="PKM27_11720"
     CDS             complement(26097..26888)
                     /locus_tag="PKM27_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR07976.1"
                     /translation="MRLKDKVIIITGSTTGIGKAIALRCVAEGARVVVHGLEKNWGEA
                     VVKTLGTENAVLHIEDLGREGAAGHLVTLALDHFGRLDGIVNNAARVVSSNIKTTDPA
                     LLRAVFEVNTLVPFTLIKAALPHLSASKGAVLNIGSVNAYSGEPNLLAYSISKGALMT
                     LTRNLGDSLHREYGIRVNQINPGWVLTETEIQRKRDHGLADDWYRQLPTVYAPSGRIL
                     LPEEIAAAAVYWLGDESGPVSGQVVDLEQHPFIGRNPPKDRSTIP"
BASE COUNT         7095 a         7271 c         6597 g         6646 t
ORIGIN      
        1 caaaattaat aaactgtcag attaaatcgt gactacttca ggtaattgcc tttagaccca
       61 aacttttttc gctgcatgta atgatatgct tccaggccct gaagtcctcc ggtatgcagc
      121 cataagaccg atgcacccgc cttgaaaaac ccccttttct gcaaatcatc caggccgaat
      181 acgaccttgc cgttatacac cgggtccaca ggtatgccgg ttttttgaaa aaattgcctc
      241 aggaagtcaa gatataaagg attggtacgg gcataacctc caagactgta atccaggtag
      301 tcaaattcat tgcggccaat acctgagccg atagcaattt tatccctaag tccaggcgct
      361 ttcagcgagg ataagacgag gaccctggtc gtccagcctt tattcaccaa ggtttttatc
      421 aatcccgttg cggtggaccc ggtgcctgcg gatacagcca ggtagtccgg cttaagtccc
      481 aggctttcca actcatggac aatctccccg acccctcttt ccgccagggg atggtccccg
      541 ccttccggca ccaggtaaaa atcgccgtaa cgatcaagga ttgcttccat cctctcgctt
      601 tccagcttca ggcggtatgc ttccctggat acaaaatgca ggaccatacc ctggtttctg
      661 gcaaaagcca gggtgggatt tgcctgatcg tcctctcccc ggataatgcc cacagatgga
      721 attccattca ttccgcatag ggctgccagg gcatggatat ggttgctgaa cgcacccccg
      781 aaactgatca aaccccggtg atttccccgg tgaaaatcat ggaggatata ttttaactta
      841 cggtatttat tcccggacac caggggatga attaaatcgt cacgctttac cagccagttt
      901 acctccccgc cccaaggtac ttggtcaacc ggactgggaa ggagcccggc tggtttcatt
      961 tccatgcaaa cgtttcctgc atgatccgga ggtccttctt taaaaattca aggacaggac
     1021 gggttgaatc cggattcacc tgggcattaa aatacaggga ggcccggaag aaatggcggg
     1081 tactgtccgt gaggaaaaac tggtatggag tggccaccgg gccttccaat aaaaaagcca
     1141 tacctcccac tccctgtttg tttttcagtt ccacctcatc cctgaaatcc gctttgatat
     1201 tgtgcttgcc caacagcttg aattcatctt caatatattt atcaaaatcc gactttttca
     1261 aaatcctgcc ataactgcaa tgcagggaag cattcaattg ggagatttca attgtaaacc
     1321 aacaggggtc cgagggtctt tcatcgaaaa aaccggtatc ctgcactacc cgcccatagt
     1381 ccgggaactc aaaacgaagc gggcagtagg cttcgtccac caacctggat ttacctaccg
     1441 gaaattcgat gcgtggaaag gcatgcggtt tcggtatgga cgctgcttcg cggcagccag
     1501 taaaaaccat caagcccgga accatcagcc ctaaccaaag ccaaaatcca ggtgtgaaat
     1561 gcctggatcg gggatcagaa aggcaggtca tcgtcttccg gaagcccgac atcttttgag
     1621 gtataatcat tccgggtggc tggagtgctt tctgctccgg ggatattttc accataggac
     1681 ctgttccccc ctgaagtttc acgacggtca aggctccgga tggtattggc aacgatttcg
     1741 gtggagtatc tatcctgccc ttctttatcc tgccatttcc ggtgggtgat tttcccttcc
     1801 acatagacca ggctgccctt gtgaagatcc cttgccaccc tttcggcaag ttgcctccaa
     1861 gctacaatat catgccattc ggtctgcgtt tgccattccc cgtttttatc gcgataattt
     1921 tcgttggttg cgagcgaaaa tttggcaacg gaagttccgc cttccaggtg tctgatctcc
     1981 ggatcttttc cgagattccc gaccagggta actttattaa tcatactctt gtattaacta
     2041 ttttcaaatc ttcaaaggta aggactcgaa cccaaattgt ttccgtggtg atccgccggt
     2101 ccagcaaggc tttctttatc catatcttct tgaagtcgct ttttatccgg tgactaccca
     2161 caagataaaa tacatctaag gcacgcctga tctgcaatat ttggatttag cagacagtca
     2221 taaaataatc tcttgtggga tgatcttaac ccgggccata acctccggaa aattaccatc
     2281 accaagcagc gaggcagtcc tcccatggct tttccctttt acaaaagaac attctgctaa
     2341 gctcttatcc cggtttttcc ccagtgcctg atttccagtt caaattggtc gaggacaatt
     2401 tcactccggg cctggagcat gccgtccccg agcctcttgc ccccccagta aaacctgcct
     2461 tcgaggctga tctggcaacc tttgtataac actctttgaa ccgaagaagc gacttgcccc
     2521 caggcgatga ccttcagcca ttgaatgaac gaagtctttg aatcttccat ccggcaaggg
     2581 cttccccatg tggccaggat aaagatgaca tactttgaac cctgttcagt ccatcggacc
     2641 ctgggttcat tggccacata acctctcagg gtgaccttat ttattttcct ttccatttcg
     2701 caaaaaccaa aaccggcacc aaatccagtg aacctggata cccaaaacca atgatccggt
     2761 cacagcgaac ctggtgccgg cgtttattta tatttccgtg gaagcggatg cggtgacttc
     2821 cagccgttct gtcttgggca ggacttcaaa acgggagagg atgatctcag tgatgtaccg
     2881 ggtagtcccg tctttggcgg tgtagttcct tgagttcaac cttccttcga gggcaatttt
     2941 attccctttg tgcaaggttt tttcaacctg ttcggctaat tcgccccagg ccaccacctg
     3001 gtgccattga gtctgttcga cctttttacc ctgcttgcct gcataattgt cgtggatggc
     3061 aagggtaaag ctggcatttt tagaaccact ttcaaattgt ttggtctggg gctctttacc
     3121 cagaaagccc gtcaattgga ctttgttcgc ttcatgtgtc atgtcgttta aattagttgt
     3181 ttaaaaaatt tggattgttt caccgggagc ctcgccccgg tcaggcaaat atgcacttcc
     3241 actcaggcct agccggacat taatcgatta ctttcgtttg ttaccgattg tacacggttg
     3301 aaaacggatc tcctcttcat tccggcttta taaaaaattg aatttctata agttatgtgt
     3361 agatggccca atttcctgca gtttcttaac aggtaaaaaa atggagaaat ctcaaaggga
     3421 ggctgggcat tcggctggaa tttttgcgat tcatcagggg aatccatgca tttgccgata
     3481 aaactttcga ccggatccct ccgaccctga attttacctc cgtaatcaaa aattctatga
     3541 cacgtttaat tcaatccgtc aaatccctgg ctttcatcct gactctgggt tttgtactgg
     3601 cgggtggttt tcttcatgcc gatgctccgg attggatcac ggagaaatac ccggcctaca
     3661 ccatccacta cacccttcag gatgaatgca ataaactcga ataccttagc cttatcgatg
     3721 agggaataaa atcggtggag tcctttttta attccccttt tcaaaaacca ttcgaagtca
     3781 tcatccatcc tgaccggcat tccctggaaa cagcctggca aaagcagctt aacctgcccg
     3841 aattcaaatc tgaatgctgg atggttgcca gcggagacgc ctacaggctg gacatgatat
     3901 cccccctgct ctgggaaagg gaatcctgtg aacaccgctt tgccgacaaa gacaagaccc
     3961 ggaagctgat catccatgaa ctggtccatg tttttcacgg ccaacggaat ataagcccgg
     4021 atttcagcga actctccggc atggactggt ttgtggaggg aatggcggcc tatgtttccg
     4081 gccaatgtga tgaggaccgg ttggcacagg tccggaccgc cctggagcaa cataaacttc
     4141 ctgaagattt ggaaaacttt tggacaggat ccttcaaata cgggctttcc ggcagcatga
     4201 tccagtacct gcagaccatt tacggcaaac aacggttggc ggacctgctt aaaatcaatt
     4261 ccctgaaaga ccttcttgct gaattggata ccactgaatc cgtcctcctc ttcggatgga
     4321 ctcaattcat gaatggtcct ggtcattgat gggtgaaggc cttcggggat ttcgcacagg
     4381 gaaaccccta tactccttcc aacagcatga tactcctgtg ctattttaaa gcatttgcaa
     4441 atattataaa cggtttttaa attaaacctc catccttctg aaaagtcaaa gcgccaaaga
     4501 cttttctcct atgctgcgca ttttaccgat tctgaccata atggttatcg ctcccaaggc
     4561 ttcctcaacc aattattata tctcctccag cgacatccgg gcaaatgact ccaaccccgg
     4621 cacctccccc cttgctccct ggaaatcctt ttccccggta aactccaaag cttcgctggc
     4681 caccggcgat acgattttcc tgaagcgggg agataccttc cgggaacaaa tgaatctgaa
     4741 agcggggcga aacctggtga ttacggccta cggcaccggg gagcttcctg tcatcagcgg
     4801 agccgaccag gtggcctcat ggacagggga agatggaatt taccaggtca agttttcaaa
     4861 acaggcgctc aacttttttg taaatcacaa agaacaggta ctcgcccggt ttccggacga
     4921 gggcagttat cttacggtcg accaggccac caacgactat ctccaggatg ccgcgcttgg
     4981 cagcatggac ctggccacac tcaaccaatc ccaggtatgc gtgcataccg cgcaatggtg
     5041 ttgggagaaa tcagggattg tttccgcttc tggcaataaa attaattatc tccatccgac
     5101 gctccgcgta ccgacggccg ggtatggtta ttttctttac ggtcatctga gttacctgaa
     5161 tacaggcaaa gaatgggttt acgattccat aaaccagttg ctttattaca aaccggtgtc
     5221 cggcaatccg aacaccatga cttgtgaagt cacggtgcgg gcttacggga tcctgccctc
     5281 gaaatcggtg accgggcttc atatcagcca gatcgccttt gcaaaacaag gagaatccgg
     5341 agtggccctg ccttatcctg aaaacgaaaa ttttaaaatc gaccagtgcc gatttgaggg
     5401 acaatatcaa catggggtgg acatccaggg aaggtatgcg gaaatcagca attgcacgtt
     5461 cagggaagtg gacggactgg ccctatttat ccatggtacc ggattcggag ccaggatcca
     5521 tcacaatacg ttccggaata tcggcccttt ccggaacagc ggcattggcc aggaaatcaa
     5581 tctttccgcg atcaaagggg ccttcgtcga cagttgccat atccatcaca acgacatcga
     5641 cagcaccggc tattgcggta ttgccatgga tgggaaatac aacctgatcg aacggaatat
     5701 catccgcaat gccatgttga tcaacaacga cggcgcagca ttaaaaagtt ttggaagcgg
     5761 ttcccaatac aatatttacc ggaataattt catcagcctc agcgatggaa acccggaagg
     5821 aaatccaccc ggcacccact tcagcactcc ggccatttat ttagacttta gcgtcagccg
     5881 ctgtaccatc caggaaaata cgatctacga ccggacccag cgaggcattt tcctcaacag
     5941 cgggacccat gacaatacag tgaccggaaa cgtggtatat ggcgccaatt attgcctgga
     6001 tttcaacggc aacccgatcc ttcccacacc aatgaccggc atgacggtga agaataatta
     6061 ttttatggtc aaaacgtccg gcgattacct catccggatg ttggacaata ccggtgggta
     6121 ccaccagggc gaaatagact acaactgcta tttccagcct tataaccctt ccctctttgc
     6181 atttctgcct ccctccacca gcctggcttt tagcacctgg aaatccacga ccggatttga
     6241 ccagcacagt aaaagcaaca cggtcagctg gaatccatcc aaacccaccg ataccctatt
     6301 tatcaatccc agcgataatc ccttgaccat aaagctgggc ccgaataaat atacagacct
     6361 ttccggggag gacctctgcg gttcgtttac ccttcagccc tatacctcaa ggatattaat
     6421 caagtcctcc accctgtgta ttcctaccag gatgaatggc aagccacgct ccggacctga
     6481 agtattcccc aacccggcca cttcatttat tcaaatccgc tcggaagaag ctattaaaga
     6541 acttgttttg acggacctga atggcaggca ggtgctgaaa gtcagtcacc cgtctgctga
     6601 aacccctatc ggtttacctg caggcctcgg ccaggggatg tacttgcttc aattcagctc
     6661 ggtttccggc acgacgatca aaaaaatcca tatcgtccgg taaaaaggtg gagtcccagc
     6721 tgcaaaatta acggccccgg aaaggaaaat cgaaaaccac accatggcct tcaaatctcc
     6781 atttcaagaa gggcgctgct catggttttc ccatgagcat cgagacccag ggaagtggtc
     6841 acgccgccgg agagcgcgtg atggcataca aacagcaggg aagagatatt gggcagtgca
     6901 tatcggatga tttcaccgcg aacggtatcc ttcagatgtt ccttcacctt ttcgaccgtg
     6961 actgttttac agagccaatc gaagtccttc tcctcataag ggatcaggga aagggtgagc
     7021 gtatctcctt tgtcccccgc gcggctgtgg gcgatatcgt ataatttcat gtggatttca
     7081 attctacctt tggaacaatc tgatctctcg gcattaaaac agaaacaatg ccgatcactt
     7141 catttatatt tttccgcacc ccgccgccgc ctgcaggacc gttggtatag agggcttcca
     7201 cttcttcacc aatcctgcgg gcgaggtcct gggtttccgt tttacccgct atccggagcc
     7261 tgatttcata aggttctgcg gcatgcctga aggcagtgcc atggacagaa cccagtccga
     7321 tcagatcaac ccggagattc tgaacaagac cggatagccg ttcgctcacg atttccgctg
     7381 ccagccgggc cctccctgcc gcattttctc cggcataggt gatttcccct tcgcccagat
     7441 aaaaagcggg atagcccaca ctgactttta aggaagaggg ttttgatttg ccactgccac
     7501 ccatcacttc cacccggtcg ggaccaagct gtttcaagcg gacagaggta aaatccgcaa
     7561 tcacatccgg tgtataatat tcgtgtggat tgaccacttc atagagcaat tgttccttga
     7621 cggtttgcag gttgagcatg ccccccgtgc cggggacttt accgaggacc gttttaccat
     7681 cggggtaaac atcggcaaag ggatgaccga gtttagccat gccggggacc ggtttcttta
     7741 gcggatcggc aaaataacct ccggtcagct gtcccgcaca ttcgagcaga tggccggtca
     7801 ccgtgccttt ccccaataaa ctaaaatcat ccgcccgcca taaaaattcg tgcatcatcg
     7861 gtgcaaggaa aagggaaggg tcagccaccc ggccggtgag gataatggat gcctccgttt
     7921 gcaacgcttc aaggatccct tccgtaccca tataggcatt ggccgaaatg agtttcccga
     7981 tggccttcag cggctgtccg gtctccaaag tcaataatcc gggttccagg tgatccagga
     8041 catcatcccc ggtcaccacc gccacctgga tcctggtatt ggtctccttt gcgatttgaa
     8101 tgattcgatc tgcagctgcc aggggattgg cagcccccat attcgtaatc agccttactt
     8161 ttttatccgg gatcaggggc aagagggacc tgatccttcg ctcgagcaaa gggtcatagc
     8221 cggaggaagg atccttcatt ttggcttttt gggcgagggc aatggtacgt tccgccaggc
     8281 attccaaaac gagataatcc aggcggcctt ccttcaccag cacttcagcc ggctcgagcc
     8341 ggtctcctga aaacccggcc ccgcatccga tccttatttt atccttcata ggtgaataga
     8401 tccggtcagg agggccataa gggtcatgat tgccgttgtc ccgaaagccc atttaaatat
     8461 aaacttttga tgatcggcca ggtcgacctc gcacagaccg gtgagcagga aggtggaggc
     8521 cgtcagcggg ctcaatggaa aaccgacggt catctgcccg agcaaagcag ctcttccgat
     8581 ttccaagggg tccatttggt attgttccgc cgcggaactc agcaccggga ccaccccaaa
     8641 ataataagca tcgggggtga ataccagact ggcaggcatg ctggttaacg cggtcaataa
     8701 aggcagaaag cgggaatatt ctaaaggaag aatgtccacc agcgaaccgg ccatggcgtc
     8761 gatcattttt gaaccggtga ggattccgga aaaaatgcct gcggcaaata tcatggagga
     8821 gaccatgaat atattggccc cgtgtgattt caggacttta tgctggtcgc caagtctggg
     8881 ataattgatc cacaaagcca gcaggcccgc taccatgaac aaaaccggtg ctgccacttt
     8941 tccgctcatc agggttataa taagcaagac ggtcattaaa gcatttacca ggataaggcc
     9001 gggtctccgc aggctgcgtt gttcttcatt caaattggat ttcagatcgt agttcaaagg
     9061 caggatcccc agccgggctc tttctttttt tccaaaataa aaagctacca gggccaccca
     9121 ggccataccg gcaattatcg caggaatatt gggattgaag aggtgcacag catcggtgtt
     9181 cagtaccgtg atggcccttg ctgaagtccc cgaccagggg accaggtgaa ggggccccac
     9241 actcagggct accactccgg ccagcaccag cctgtttatc tttaattctt tgtaaatcgg
     9301 gaggaaggcc gataagacga tcataaaagt agccgtgccg tccccgtcca gatggaccag
     9361 catggtcagt acggccgtgc ccagtacgac cttcaaagga tctcccttga cctgcctcac
     9421 tatccggttg atcaccggat caaacaagcc ggcgtccagc attacagcaa aataaaggat
     9481 ggcaaagatt aaaagaatgc ctgtcggggc tacctgttga ataccggtaa gcatcatccc
     9541 ggacaattct ttaacggaaa aaccggcgac aagggcggcc aggagcggga ccatgaccaa
     9601 agccgtaatc accgacatct tccgggtgaa gatcaggact aaaaatacca ggatggttga
     9661 aaaacccaga atggctaaca tcaggtccgc ttgttataat attaatcaaa agcctgagga
     9721 taaaggtaaa aaaatgcgaa atgcgtacct tccgtcaatg gaggtcatcg gtacgcgggg
     9781 tctttccaag tctttcggac tcaacagggc cctagaccaa atatccctga gtataggatc
     9841 gggagagata tacggtttcc taggactgaa tggcgccgga aaaactacat tgatcaggag
     9901 cctccttggc atgatcaacc ctgaccaagg ccaaattcac ttgtttggcc gggaacttaa
     9961 ccccaaattt gaccaatgga ataaaattgg atacctcgta gaatccgcca aggcctatcc
    10021 tgaacttacg gtagctgaaa acctaaaagt atttggcctc cttcgtcgca ttaaggtacc
    10081 tgccctggaa gcggatgcga ttgaacgtct caagctggct ccataccgaa acaccaaatc
    10141 aaaagccttg tccctgggca atcttcagcg ccttggactt gcaaaggcgc tttttcacaa
    10201 gcccgccctc ttgatcctgg atgaacccac caacgggctg gaccccgagg gaattgtaga
    10261 gatcagaaat cttttactcg acatggccag gacggggacc accatattca tttcaagcca
    10321 tatcctggca gagatttcca gacttgccga caggatagga attatccata aagggaagct
    10381 ggtccgcgaa ctccatgcgc atgaattgaa ccggcaaagg acgaccagcc tgcggttgcg
    10441 gacaagggat aaccctaaag cccgggaagc ccttttgaat caaggatttc cggcaattca
    10501 aatccatcca tcgtggattg aaatcaaaga tgaacgggca attcagaatc cttcatccct
    10561 cacccgatgg ttaagtctgc aggatttgcc tcctgaggaa atctatatct tcaaggaaga
    10621 cctggaacat tttttcctcc ggaccttaca aattgaaaat caggatgact gacttttccc
    10681 atgctgtgcg ggcagagtgg cttaagctca aaggcagccc catggtgcgg ttcagcttca
    10741 tagcctttgg gctggctcct ttcatggtgg gactgttcat tatttttatc caataccccg
    10801 agctggcagt aaaagccagt tccttctctg ccaaagcaca ggtcatgaat atccaaccga
    10861 attggccttc ctttctggcc gtcctgcaac aagcgctcgc tgtgggcggc atcctggttt
    10921 ttggttttgt agccagctgg atttttggcc gggaattcag cgacggcacc ttaaaagatc
    10981 ttttagccct tccgatccgc agaagcacct tggtccaggc aaaatttgcg atttatttca
    11041 tttggtgcct tggcttgtcc ttttctaatt tggtcatagg cacggggatt ggtatcctgc
    11101 tgcatttaaa ggacttctcc gccggccctc aaagcctgac aaactatgtt atcaccacat
    11161 tcatggtcat tttgtctggc tccccggttg cttttatagc cagttggggc aagggatact
    11221 tagcccccct ggcttttgta gcgctcactc tggtactttc gcaaatcctt gcagcagcag
    11281 gattcggcca atttttcccc tgggcagtgc ccggtttatt cagtgatatt tcaggaggca
    11341 caaatctctt gaccggatgg agccatgtca tcttgtgtct gaccggtatc accggttatt
    11401 tcctcacccg catatattgg accaaggcgg atcatatttc ctgaaccctt ttcagatgct
    11461 cttctttggg tatattcccg taaatttgca ttattactcc ccccttccgg ggtgcggtct
    11521 gcaccaggcc gattttgtga agtaacataa agcactatta aaatttactg acatgattac
    11581 agtgattata ccggcattga acgaagaaga ccacatcggg agcgtagtcc gttttgcgag
    11641 gagccagccc ctggtaaacg aagtcattgt cgtggacgac aaatcgtccg accgcaccgt
    11701 ggccatcgcc caggaaaacg gcgccaaagt gatcaccagc accaaactgg ggaaaggggc
    11761 atctatgaaa gaaggcatcc tttttgccac caatgaaatc ctcactttcc tggacgggga
    11821 cattgacccc tatccttttt acaccatcaa actgttgacg gaacccttgc tcaaaggtga
    11881 ggcggaattt gtaaaatccg ctttcagccg caatgcgggc agggtaacgg aactggtggc
    11941 caaaccctta ttgagcattt ttttccccga cctgcttgct ttcagccaac ccctcagcgg
    12001 catgatcgca ggtaaaaaat ccctttttga aaaactggat ttcagggatg actacggcgt
    12061 cgacatcggg atcctgatcg acatgcacct gatgcaggtc agaatgaagg aagtggaaat
    12121 cggttacctg gaaaacaaaa gcaaaccctg gcaggccctg ggaaaaatga gcaaggaggt
    12181 cgcccagacc atcatcttta aggccgcttc ctctaaaaat cccaattaca atgtcgaaga
    12241 actcggtgtg ctgaatgaaa tacgttccca aatggaattt gcactggtag atcatctgag
    12301 caacctgagt aaactcatgg ccttcgatat ggataatacc ctgcttcgcg gccggtttat
    12361 cgatgcctgt gccaaccggt ttgactttaa aaaaccactg atggatatcc ggtcctcaga
    12421 aaaagacgaa acggtgctga ctaaacgaat cgccaatctc ctgaaaggca gaaccattca
    12481 ggaactcatc gaaaccgtcg aacagattga actggtggaa gggaccaaag aaattgtaac
    12541 ggaacttaaa aaaaggggtt atatcgtagg aatcataacg gacagttacg attgcattgc
    12601 caaccacatc aaaaataaac tgggaatgga ttttaccttg gccaatgaac tggagttttc
    12661 caagggaatc tgcaccgggg aagtgaaaat cccatccttc ctttataacc atcccaaaag
    12721 cctctgcagg cacacccttt gcaaaaccaa tgccctgatc agcatcctgg aaaagtacga
    12781 catcaaaatg gaaaatacca tcgcgatcgg ggacagcaac aacgatctgt gcatgatcaa
    12841 agaggcgggt atgggcattt ccttctgttc ccgggaagaa ctgctcaacc accatgcgga
    12901 cctggtcatt tccgaacctt cctttgctga attactggaa gtgacctgac gactgcagcg
    12961 ccgcttaaaa aatttgaaag ggcatttccg gatgaacaca aacggatgga agactgtttc
    13021 ttttacatac ccttcttttg aatcaagaaa agtcatttca aaaaatgccc aatatcctca
    13081 ttctttcatc cagtgtccgc cttggccgca acagccaccg cctggccttg tttttccggc
    13141 aatacctgct tgaaaaccaa ctggcccagg ctgaaatatt ggatctgaac gaatatcaat
    13201 tccccatatt tgaagaacgg ctgatgtacc agaccaaccc ggatcccagg gtcctggagt
    13261 ttgccgaaaa aataaaagca gcggatggag tgatcatcgt gacgccggaa tacaatgggg
    13321 gttatccggc cagccttaaa aatgccatag atgtgctgga gccggaatgg tataaaaaac
    13381 cggttgccct ggtcacggct tcagatgggc aatttggagg ttctcaggtg atcacggccc
    13441 ttttgctgac tttttggaaa ctcggtgccg tggtggttcc ggcccgttat ccggttccca
    13501 aagtccagga agcttatggc ccggacggaa gccccaccga tcctgcaacc acaaacaaat
    13561 tcgccaaaaa gtttataaag gaactgttat ggaccgtaaa agcagtgaaa gcagggatga
    13621 aggaaacgag tcccggagca taagcattgg gataaaacag ggatccgatc catccggttt
    13681 tccaacttaa cccaggtttc aaactccggt acaaggctta tctttgccgg gtgaaacgcc
    13741 gcctgaccag cgaagattat ttgagggaat tagaagaatt cagacggttt ccggacatga
    13801 atccggggcc tatgatccgg acaaaccttg aggggactgt attatcctca aacgtcgcag
    13861 cccagaattt attcggtaag gactttaacg gcaaaaactg gaaacaaatt tgtccgggca
    13921 tcaccacaga agtttggaac aaaatcctgc aaacggaata cgcgttcccg ctggaggccc
    13981 agttcagcac cttgtcctat atattcaatc accgggtgga tcaattgggc aggatggtat
    14041 ttgtattcgg taccgatatc acggttcagc gttcggccga aaaacaaatg aagcaggata
    14101 aaaaaatggc taccctcggt acgctggcgg ccggcattgc gcatgaactg aacaatcccg
    14161 ccgcagcgac ccggcgtgct tctcaacaac tgaaggaaac ccttcataaa atagatttga
    14221 accgccaaaa cctttatacc gtcaggcttg gcccggggga aaccgatctt atgcttgaac
    14281 tggcccaaaa agcaagtgaa ttcacgaaac aacgcagcag cctgaccgcc ttgcaaattt
    14341 cggataaaga aacggaaacg gaggaatggc tcgaacaact cggctttgaa aatgcatggg
    14401 aaatcgcccc ggccctggta gccatcggga tcgaaaaaca atttatagac tcccgggcaa
    14461 agtccctaag ccccgatgcc ctgaaagcaa tccttcaatg ggtgggcctc ctggtacccg
    14521 tttaccagct gcttcatgaa atttatgaag cgtcttcccg tatttcggac atcgtggttg
    14581 ccttgaagaa ctattctttt ctcgggcagg cgccggtcca ggcggtcaat atccatgaag
    14641 ggatcgacaa taccctgctc atcctcaaac acaaacttaa agcaggcatc accgttcaaa
    14701 aacggtattg cgaagacctt ccccccgtca ccgcctacgg aagcgaactc aaccaggtct
    14761 ggaccaacat cctggataat gcggccgacg cgctcaaagg gaatggagag atcatcatcc
    14821 gcacttataa gctggataat atggctacgg tggaaatcga agacaatgga ccgggcatac
    14881 cggaaaccat cttacccagg atctttgacc ctttttttac caccaaagaa cccggaaaag
    14941 gcaccgggct gggactcagc acttcttacc ttatcatcac agaaaaacac aagggacatc
    15001 tgagggccag gtcgaggccg ggttcgaccg tgtttaccgt tcaactgccc tttaaccagg
    15061 aaaccaacac ggaacctcca caaggttaaa tcgaattcac tctaaaccat gtccaaacct
    15121 gtaatattca ctgttgacga tgatctggaa gtcctggccg ctatccacag agaccttcgt
    15181 caacattata aaaatactta ccggattctc aaagccaata ccggcaatga agttttggag
    15241 gcggcccgcg aactcaaaca gcgtagcgtg ccggtagcct tatttctcgt ggaccagcgt
    15301 atgccggaca tgaccggtac cgaactgctg aaggaactgg tcaaatggta tcccgaggcc
    15361 tgcaaagtct tgctgaccgc ctatgccgac acggaagccg ccatttccag catcaacgaa
    15421 atcggcctgg atcattattt gctcaaaccc tgggatccgc cggaacaaaa actttttccc
    15481 gtcctggacg acttgctcag taaatggaac acccgggtga aactgcccta tgaaggcatc
    15541 cgggtagtgg gttcacgttg gagcgccagg agttatcaat ccaaggaatt tctcagcctc
    15601 aaccaggtgc cttaccagtg gctggatatt gaagaggatg gccaaacccg ggaactgatc
    15661 ctgccactga gcaacaagct gaaaaaacta cccgtcatcc tgtttccgga cggtacttac
    15721 ctggtcgagc ctaccaatct tgaactggct gagaaagtcg gcattcaaac ccgggcgcaa
    15781 caacaatttt acgatctcgt ggtgatcggt agcggtcctg ccggccttgc gaatgcagta
    15841 tacggcgcct ccgaaggcct tcgaaccttg ttgatcgaac ggaacgctcc cggaggtcag
    15901 gccgcgacga gttccaggat tgagaactac ctgggatttc ccgccggcat cacgggagcg
    15961 gacctggccc agcgcgctgt agcccaggca aaacgcttcg gcgctgaaat gcttaccgcc
    16021 caggaagccg tatccttccg gcgtgaagat ccgtaccgct atgtagtgct ggccgatggc
    16081 agtgaaatat gttgttatgc cattgttttt gccaccggca tgagcgtgcg caaactggaa
    16141 gtcccgggaa tagagcggtt tcagggcatc ggggtatatt atggggcggc catgagcgag
    16201 gcggccattt ataaaggaaa aaacattggt atcgttggag gggcaaattc agccggccag
    16261 ggcgctgtct ttttttcccg ctatgcgcag agcgtgacca tgctcatacg cgctgaatca
    16321 ctccggcccg gcatgtcgga atacctggta gaccgcattg aagacagccc aaacatcagg
    16381 gttattccgg aggtggaggt gatcgccgtg gatggggacc aggctttgga aaaagtaacc
    16441 atccgtaata tcaaaaccgg agaggaaaag ctactggacc tggccgccct gtttattttt
    16501 ataggatccc agccccattc ggagatggca gcaggctttg tcgctcgtga tgccaagggg
    16561 tttatcctca ccgggcccga ccttcccagg accggcaaca aggtacccgg gtggacgctg
    16621 gaccgggatc cctttttatt tgaaaccaat gttcccggag tattcgccgt gggggatgtg
    16681 cggagcggag ccaaccgaag agtggcggcg gcggtaggtg aaggttcggc atccatctac
    16741 atggtgcacc gttatctcac taccgtctag gttaagaaat atacccagcc atgtcggccc
    16801 tcctaaaaac ccgcgtcgaa caaacccttg ggcaagccat tcctctcacg gaatttaatg
    16861 cctttctgga ccttctgtat tccaaatcat tcgataaaaa aagtgtgctg gccgaagaag
    16921 gcaaggtttg caagaacatt tatttcttcc agaaagggtc ggcttattcc tatatcctgg
    16981 acacgaaggc ggaaaaacac gcgattcagt ttgcccttgc cggttactgg atttccgatc
    17041 tctacagttt tttctccggg cggccgggca aatacaatat tgaaaccctg gaaccttcag
    17101 aggtgctggt attgaataaa caacagtttg aggcggcttg cgataccatc ccggttttta
    17161 accgattttt tcgcatcctg atccagaatg cctatgtctc gctgcaattc cggctggccc
    17221 gtaccaacag cgaagacgcg gaattccgtt accgcgaatt ttcatctttg catccggatt
    17281 ttgtacaaaa tattccgcaa tacctgatcg cttcctacct gggtgtaaaa ccacaatcac
    17341 tcagccggat caggaaagcc ttattaaaat catcggcaaa gtgaacctgt ttgagattca
    17401 gcccaaaccg aggtcgggtt ttaaacttct tcagcccaaa gaattcggaa atggacgagt
    17461 ttatatgaag cctctttttg acagaatcag gaattaatga aagataatga gtaactttgt
    17521 cccctacccg agaggcccaa atatcattgt atatctattt tttatttttc aaatcaaaca
    17581 tgtcatttca atcgttacaa atcgtaaagc cgattctgaa ggctttagag gaagaaggtt
    17641 ataccactcc cactccaatc caggcgcagg ccattcccat agttttacag ggcaccgacc
    17701 ttctgggttg tgcacagacc ggaaccggaa aaacggccgc ttttgcaatc cctattctgc
    17761 agatcctggg cgccgccccc cacaccccga ataagaaaag aaaaataagg agcctgatcg
    17821 tcacccccac ccgtgaactg gccatccaga tcctggaaag tttcagggcc tatggacggc
    17881 ataccggatt gaacttcacg gtcgtttttg gcggagtcaa tcaaaatcct caaacctcgg
    17941 ccctgcgcgc gggagtagac atcctgatcg ccacaccggg caggttgctt gacctcatgc
    18001 aacaaggttt tgtgagttta caggatatcg aaatttttgt cctggacgag gctgaccgca
    18061 tgctggacat gggatttatc aacgacgtta aaaaaataat agcccggctt ccccaaaaaa
    18121 ggcaatcgct ctttttctcg gcaaccatgc ccccggagat taccaaattg gccggcacca
    18181 tcctccacaa cccttccagg gtttcggtga ccccggtctc ttctacggtg gaaatcatcg
    18241 aacagtttgt tttttatgta gaaaagggca ataaaaatgc cctcctcgtt gaacttctcc
    18301 gggacaaaaa aattaaaacg gccctggttt tcacacgaac caaacacgga gcggacaaag
    18361 tggtcagaat gctgcatcag caccgtattc ccgcagcggc catccatggc aataaatcac
    18421 aaaacatccg ccagcgcgcc ctttctgaat tcaaggcgca gaggacccgg gtcctggttg
    18481 ccaccgatat tgccgcacgc ggcattgaca tcgaagacct ggaatatgtg attaattacg
    18541 acattcctaa tatttccgaa acctacgttc accggatcgg aaggaccgga agggcgggag
    18601 ccgggggcac ggctttttcc ttttgcgacg ccgaagaaac cgccttttta aaggacatcg
    18661 aaaaactgat tggcaaaaag atcaaggtta tcgaaaatca tccctaccgg atgcagttca
    18721 ccggcgcgac cttacccaaa aaccttccca gaagcaaacg gagttcaggc aagtccagga
    18781 acagtccggt caccggcggc cgcatctccc actaccgcag ccccttgaag gcgtcctggt
    18841 gagttctctt ggaaactaag gctcctgtgt acaaatggga taccgggtac gcgatattgc
    18901 ttcattcgcc tttcgccttt tacccttcgc ccctgaacat cttaacctat gtgaacgact
    18961 ttccccccta tccccctgca actttgtgtt attaattcaa taaaaacaca aagcaatatg
    19021 aaaaaactga ccaaatgggt cctcgaccct gctcacagcg aactgcagtt caaagtaaaa
    19081 catatgatga tcaccaacgt caacggggag ttccggaatt ttgacgcttc ggtcgaaacg
    19141 gttgatgatg atttcaccaa aggaaggatg catgtggtga tcgacgccac ttccgtattc
    19201 accaacaatg ccgaccggga taatcacctc aaaagccctg atttttttga tgcggaacaa
    19261 tttcagaacc tggagtttga aggaacttcc attaaaaagc tggacgaaga aaactatcag
    19321 ctgaaaggcc tgcttaccat caagggcatc caaaaaccgg tggttctcga tatggaatat
    19381 ggcgggattc aaaaagatcc ctggggaaat gaaaaggccg gattcaccct caccggaaag
    19441 atcaaccgga aagaatgggg actcaactgg aatgccgccc tggaagctgg cggtgtgctg
    19501 gtaagcgaag atgttaaaat caatgcggaa gtccagttaa aaagggcctg attggtccgg
    19561 catggcctcc gcaaggccat aaatcctgcg gaaagaatat tttagtcttc atttattaaa
    19621 atacaattct tatgaaaatt ttggaaaaca aagtggccat agtcaccggt gcgggttcgg
    19681 gtatcggacg cgccattgcg ctgctttatg ctgctgaagg tgcaaaagtg gtggtttcgg
    19741 acatcgctga aaaaggaggc ctggaaaccg tttcccagat aaaagctgca ggcggacagg
    19801 ctatttttgt caaagccgat acgtccagcc ctgaggataa taaaaacctg gtggaccaga
    19861 ccgtaaagga atatggagga ctgcacattg ccgtcaacaa tgcgggcatt ggcgggccgc
    19921 ttggcccgac gggagactat ccgatcgacg gttggaaaaa agtgattgac atcaaccttt
    19981 ccggcgtatt ttatggcatg cattaccaga tacaggctat gcttgcttcg ggtgggggca
    20041 gcatcatcaa tatggcctcg atcctgggca tgacgggtac gaagggatcc ccggcctatg
    20101 ttgcggcaaa acacggggtg gtcggcctta cgaaagcagc agcgttggaa tatgccacca
    20161 gcaatatccg ggtcaattcc atcgggcccg gttatatcct gacccccctg gtgactaatg
    20221 ccctggacga agccaccctg aaagcactgg tgggcctgca cccggtaggc cgtttaggaa
    20281 aatctgaaga agttgcggaa cttgccttat ttttaggatc cgacaaggct tcgttcatca
    20341 ccggctctta ttacaatgta gacggggggt acctggccca atagtacatt ccaataaaag
    20401 actcactcta accaaatctg ccattccgga tctccgtgtg gcagattttt ttttgtgaat
    20461 ttttcaggag gagttgaccc ggacgagcaa gtttgggaaa ggctaagcat agaccttgaa
    20521 atgaaaattt agtaaggctc aaatgaaaat cactgtaaac accaaggtcg tcgggtttgt
    20581 ccaggttttt ctttgcacaa tttccggctc tccgcattct ttgcggttaa tcaattatcc
    20641 ctgtattttt ataaaacatt attaatgaat gaaaaataag ttcaggcgga tccccatccc
    20701 ccgtttcccg gtgcagcggt tccttattct cctggcaagc tgtattttac cgtggatgag
    20761 caaagcacag cccgtgcaaa aaaattataa actggccatg gtccaaatga aggtggaagc
    20821 cggcaaaccg gaggtcaacc tcgcccgggc caccgaacgc atcgccgaag cggcccggaa
    20881 tggagctgtc atcgcccttc tccctgaagc catggacctg ggttggaccg acccttcagc
    20941 aaaaacgaag gcttatgcca ttccttcagg ccccacctgc caaagcctga ttcgcgccgc
    21001 ggtcgaaaac aaaatatacg tctgcgccgg gatcatcgaa aaggatgagg aacgcaccta
    21061 taacgcggcc gtgcttatcg accctaccgg ccaggtcctc ctgaaacacc ggaaaatcaa
    21121 tgaactggat atagcccacg atctgtatgc gaccggagag ggactgcagg tagcccatac
    21181 cccgataggc accctgggcc tgtatatctg cgccgacgcc acggctaaag gaaatacttt
    21241 gtccaattcc cttgcttata tgggcgccga tatgatcatc tcgccctgcg cctgggctgt
    21301 gcctcccgga tttgacaatc agaaaacccc ttatggggat acctggcgca aggtctacag
    21361 gcctgtaagc gctcagtttg acctttgggt ggttggggtc agcaacgtgg gaaaaataga
    21421 agccggccca tggaaggact gggattgtat aggcagttca ctggtttacg cgcccggggg
    21481 catagaagtt ttacaggccc cttttggagc ggaggcggat accattattt atgtcgatat
    21541 cgccctcaaa gaccggcccg cccgcgggac gacctggaat acctactggc agaataaata
    21601 agctttatcc ccgttccgaa tccaagccat acgatgaaaa gcagccagga tgtcttttcg
    21661 gtgcggaatg ccctcccttc agaatttgca gaaatcggtt tgctgatggt ggaggcctat
    21721 tcccaacttg aaggatttcc caaagaaacg gagcaacccc actattacca gatgctggcc
    21781 aatgtaggtg acctgacggc caaacccgga gtggaactgc tggtcgcggt gtccggagag
    21841 ggggagccgg cgggggccgt ggtctatttt gcggacatgc tctattacgg ctcgggcggc
    21901 agtgcgacca aagcaaaaaa cgctgccgga ttccggctgc tggccgtaag tccgaagttc
    21961 aggggccggg gccttggcaa acttttaacc atagcctgca tccagaaaac cattaaaacc
    22021 ggacttggcc aactcatcat ccatacgacc cgggccatgc agaccgcctg gaaaatgtat
    22081 gagtcccttg gcttccaaag atcggaagac ctggattttt tgcagggcga ccttcctgtg
    22141 tttggattca ggctcaggct taaggaataa aaaaaatccg tttataaaat attaaacccg
    22201 gggccatgaa gcagatcgaa acacagatca tcatccatac caggcccgaa aaagtctggg
    22261 ctgtacttat ggatttttca tcattccccg attggaatcc ctttatccgg cttattggag
    22321 ggggccatca ggccggggaa tccctgaggt tgaccctgaa agttccggat ggaatggaga
    22381 tgaccatcgg cccacagatc ctcaaggccg aacccaacaa agaattccgc tggaaaggca
    22441 aactgatcat ctccgggctt tttgacgggg aacattattt taaattgatt gacctcggcg
    22501 acggcaggac ccagtttatc catggggaac aattcagcgg gatcctggta ggcctgtttg
    22561 gtggcacggt tgccaaaacc aaaattggtt ttgagaaaat gaaccaggcg ctgaaaaaaa
    22621 gatgcgaaga gattaaaaat taaaacacct gcaacgcatg gtcacggcgg atcaatcccc
    22681 tggcatccac cttcctatgg tttttggagc aaccggtgcg cgagttgttc agcctgcgcc
    22741 cggatttcgg gtgcggccag ggtctcccat ataatgcccc ttaacggaaa tcccagggta
    22801 tataaaatat tgtgggcggt gccgtcagaa tgaaggatgg cgccattggg cagggcattc
    22861 atacccaggt gaaaaggatc aggctgcaat tctgccttat tcaacaggtt tttgatcaag
    22921 ggatggtcta ctttaaagta atccgtttca ggccccatgc agttgatcac ccgtggggag
    22981 gctatggttt caggccgcga ttttccggcg ggtatataaa tcatttctaa atgatgatct
    23041 tgcggcacca gatcggtgat gcgtcccctg actacggtca actgccccga ttcctgcagc
    23101 cgcttaacta cctcttcgct ttccgggggt atccggctgc gaagaatttc aaaataccgg
    23161 aaaacatgcc ttagaaacct cgctttttct tccgaggtca ggttcatcca gagttcctgg
    23221 gtatagggcc tcattgaatc catgaccgca ttggcatcct gtcccaattc tttggatttt
    23281 ttaatgtgaa ggcgcaccac ccttaaaatc ccggcaatag aattctgttt cattatttcc
    23341 tccctgaaag atgggtattc cttaaaagtg gaatgagcca ggggaaagag tccatgcctg
    23401 gacaccgcta tcattttgcc ttcatgcctg atcctgttca ggcggaccag gagatcgacc
    23461 gtggtttgcc cggtaccaat cagcacgacc gtctcgtcct tcgagatgga atccagtacc
    23521 tggtcgggcc aggggttctg aaagtaatgc ctgctttgaa taaacgaacg gtttgccaac
    23581 agcagatgcc ttggaggaaa atttcccagg gccaaaacca cctggtctgc atggaaagca
    23641 tgattctcgg taaaaacctc cgcctttcca tttcggattg aaatatcggt cacttcccct
    23701 tgaattctgg tcatcgcagt acccttggct tttttcttca tcgcctgctg atacaattcc
    23761 tggatgtatt cccgatataa aagccgggga agaaaatccc attccccggc ggataggcct
    23821 ttctttcgaa cccattgtaa aaaatgtcca ggggcggaac tgtacgcacc cattcgtccc
    23881 gcggtcacat tgagcaacaa tgcttcctcc cggctgctgt aagcgggtcc cagatccctt
    23941 tccggattgc ggtcaatcag ggtaatatgg accggaccac cgctccctcc ctgttcaatc
    24001 aaattgatgg ttaacaaggt ccccgacaat ccagccccaa tgatgacaat atttttcatg
    24061 gcatatccgc gttgagcccg gttgaatcac ccggttgaaa ggacctcctg tacatcccgg
    24121 ataaatccca aggccttttg ttctgctttt acggccaatt cttcaaagcg gtccaaatcc
    24181 ggatgttccg ccaatacgct ttcgacaatt gaaaaacggt cccggatggt tggagggata
    24241 aagtgtttat tccaatcgct gcccctgaat agctcccaat aggctttctg gatgtttttg
    24301 ttctgcccgg caagccatat ttccaggcga tcgtccgggt aaacatagac cagggcaatt
    24361 ttcagcttaa gctttttaag atcaaggggc gtgaaaggaa aataggtaat ggtcccttcc
    24421 ccgacataca attgcccggt gtaataatcc tcccggtatt ttttttcaag ggctgctttc
    24481 agttgcttca tgaaagaaat cacaccggga tttggcgact tctgttttga ctccatgtcg
    24541 actgtacatt cagaggttta tcaaaattac taaacggcat ttgttcagtt catggtttgc
    24601 agaacctgtc agaaaccagt cttttccaga tattgcttca gcgtaaccag caccttgggc
    24661 catgcctccc gcacagcctg ataataccaa tcccagtcct ctccgtcccg gtaaccgtcc
    24721 tggcaaaggt atagttcggt ggttccgccc aggtcctttg cccgcatagt caggctcatg
    24781 gggccgaaca agggcttccc cggattgaga tataccagat ctccgatccg gagcacatgt
    24841 tcagggtcat attccctgac cagtccggtc atcacgaagg agaaaccgtt tccgtccgca
    24901 ctccaggcca gggtgtacag gccacccaca cccagtttaa tgagggaacg atccacaccc
    24961 caccagtctt tcagcatgtg ttcgctggtg aaggcctcca gcgttttggc cgggtagttt
    25021 tcaataagaa tgctggattc cacttttttc atattctctt tagggggtct gcatttcgat
    25081 ccaaggatca aaagccggtt taaaataccc ggttcatggc caatacttgt atattttttc
    25141 cctcagaaaa gcgacgctcc gctccagttg ttccatgccc tccaccccgt cttctatact
    25201 gatccagcca tcaaagccca cccgcttcaa ttcactgaag atggcatcgt aatcattgag
    25261 ccccttcccg atctctccat gactcagcct ccgggcataa cctaccgctc ctccctcttc
    25321 tttccttaaa tcttcgaggg taccctcttt cagaaaccgg tcgctggcgt gcatggtcac
    25381 cacccggccg gaaacccggt acaacaattc caggggatct tcccccgcga gataggtatt
    25441 gcttggatca taattgaccc caaaattggg gtgccttaca cggtccacca gtttacagaa
    25501 cacttccatt ttctgggcaa attccgggaa ctcccagaaa tcgtctttat aatgattttc
    25561 caggatgagg gtgatgccgc gctcccgcgc ataaggcagg caagcctcaa tgcattcggc
    25621 agccagggat atgccctgct ccgtgctcag ctcgggccgg cgttgtcctg acaagaccct
    25681 gcaataagat ccgcccaggg cataggtcat atcgatccat cttttttctt ttgttatttc
    25741 acgggcccgg aattctggat caggatgggt gaaatccggg gaacaacaca tcatcgggat
    25801 caccttaccc tggtcttcca cccgcttccg gaaggtcgtc cagttttttt catctgccat
    25861 ttccaaaaat ccggcgtacc attcaaggcc atcgatgtcc agcgcgcagg ccaggtcgat
    25921 ccattcagat aatttcatgt cgcctgtttt gcagagatcc tgcatgaagg ctttggggaa
    25981 ggcggagagg aggggcatgg aggaggtttg agggttgagg gttgaggatt gggggttgag
    26041 gtcgtgcagt cacaaaacaa ttttacaatt taacaattta acaattcaac accgtatcag
    26101 ggaatggtac tcctgtcctt cggcggattt cgcccgataa acgggtgctg ttcaaggtcc
    26161 acgacctggc cgcttaccgg acccgattca tcccccagcc aatacaccgc ggcggcggca
    26221 atttcttctg gcaagagaat tctgccggat ggggcgtaca ccgtgggcag ttgccggtac
    26281 cagtcgtcgg caaggccatg atctctcttc cgctggattt cggtttccgt gagcacccaa
    26341 cccgggttga tctggttgac ccggattccg tattcccggt gcaggctgtc gcccaggtta
    26401 cgcgtgaggg tcatcagggc ccctttggag atgctgtaag caagcaggtt gggttcgccg
    26461 ctgtatgcat tgaccgaacc gatgttgagc acggctccct tcgatgcact gagatggggc
    26521 aaagccgcct tgatcagggt aaagggaacc agggtattga cctcaaaaac cgcgcggagc
    26581 aaagccgggt cagtggtttt aatattggaa gagacgaccc gggcggcatt attgacaatg
    26641 ccatccaacc ggccaaaatg atccagggca agggtgacca ggtggcccgc agccccttcc
    26701 cggcccaggt cttcgatatg caaaaccgca ttttcagtcc ccagcgtttt gacgacggcc
    26761 tccccccagt ttttttccag gccgtggacc accaccctgg ccccttcggc aacacagcgc
    26821 agggcaatgg ccttacctat cccagtggta ctgcccgtga tgataatgac cttatccttt
    26881 aatcgcatgg aacggcggct ctttcaaagc gaaaatattg aataaaatcg tattgcatcc
    26941 tggttaagtc gaagtttagc ttcagcgttt cccgccaggg caacgggtat agactggtct
    27001 cccacaaagc cacgaagtac acaaaatgta ggctattcat agatatcggc tcaatcccaa
    27061 acatccaagt aaattcttcg tacccttcgc ttcgctgtgc cgttgtggga accctaacca
    27121 ttgcggccaa cgggcataaa ttggtctccc acaaaggcca caaagttcac aaaatatagg
    27181 ctattcattg ataccggctc aatcccaaac attcaagcaa attcttcgtg cccttcgctt
    27241 cgttgtgccg ttgtgggaac ccaaacccta gcgggaaaca aggtatagac tggtctccca
    27301 caaagccacg aagtacacaa aatgtaggct attcctttag gcaggtcata cgggtaggtt
    27361 aattcccacg gtttattccg gcatcggaga tgcctgacct taatgctata aaaataagaa
    27421 caaaacaatg gcttcggaga agctcaacaa tcacatccca attaaaaaat taatattatt
    27481 tctttgatat aggagatggt ggtggccatt atttgggtgg gggatccgta gggacaataa
    27541 ggttgatatt cgacgatgtt gggcttctcc gaagccgggg ttttacttga atgaatatta
    27601 aaaacgtgg
//
LOCUS       DAOQXT010000063        27247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2056_length_27247_cov_7.068550, whole genome shotgun sequence.
ACCESSION   DAOQXT010000063 DAOQXT010000000
VERSION     DAOQXT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 27247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27247
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2056_length_27247_cov_7.068550"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..463
                     /locus_tag="PKM27_11725"
     CDS             212..463
                     /locus_tag="PKM27_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07977.1"
                     /translation="MMNKILWHAVSISALVRTRPENNAPVVKSVSNGIWLGVMAEMGE
                     WIYVVGKEAIGWVLRKEITTCDAQALHIRQDEAGWVVGY"
     gene            939..1460
                     /locus_tag="PKM27_11730"
     CDS             939..1460
                     /locus_tag="PKM27_11730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HNR07978.1"
                     /translation="MNRRNTIKHLFAASGALTALPSWARGWTASSFKTFRSSFNKAEQ
                     EILSAVVDTIIPAGNAIGAKSVGVDLFLQKLFDRCYEKEVQDNVKKQLQALESKAQAG
                     HQTSYAACPSALREQLLLQFASSEIKEEKDFFTLIKSETIRGFNTSEQVMVQYLKYKV
                     APGHYYGCVDVNA"
     gene            1533..3236
                     /locus_tag="PKM27_11735"
     CDS             1533..3236
                     /locus_tag="PKM27_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNR07979.1"
                     /translation="MTNFNIDSAKKRSYDAIVIGSGASGGWAAKELCDHGLKTLVLER
                     GRDVKHIVDYPTASKAPWEFEFRGTVPLEKRAGYQFGRFMQEATLHWALKDDEQPVVY
                     EKPFRWFRGYHVGGKSLLWARQTQRWSDFDFEGPARDGFAVDWPIRYKDLAPWYSHVE
                     KFAGIAGNKDGIAELPDSEVMPGFEMSCIEQYFKEKLKAHFPDRHLIQARCAHITDPQ
                     EIHIQQGRSKCQNQAMCNRGCIYGAYFSSNASTIPWALKTKKLTMRPDSVVHSIIYDE
                     KKGRATGVRVIDRHSHEMIEFHAKIIFVNASTINSTAILLNSKSNRFPNGLGNDNGLL
                     GKFLSWHNYRGKANAQFEGFADKRTDGRNPSNSYIPRFRNVRKQEMDFLRGYAIGIGG
                     GRGTSTDTSMIGDQLRENILNPKYGRWNMSSWMMGECVPIESNHMRLHHELKDKYGIP
                     QIVLSCEWTENDDKMVADYVEQSKAMFEAAGFINITANDTKSAPGSDIHEMGGVRMGH
                     DPKTSLLNGWNQMHHCKNVLVSDGACMTSTGTQNPTLTFMAITARAANHAVEELKRRN
                     I"
     gene            3777..4160
                     /locus_tag="PKM27_11740"
     CDS             3777..4160
                     /locus_tag="PKM27_11740"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNR07980.1"
                     /translation="MNSIKLALALLLAPLFFQMANGQETGQMVRLAKLVIDSTQLEAY
                     QVILKEEIATSVKLEPGVLTLYAVAEKANPNHITILEIYSGIEAYEKHIQTPHFLKYK
                     DGTKHMVKSLELIATIPLIPEMKIK"
     gene            complement(4271..4438)
                     /locus_tag="PKM27_11745"
     CDS             complement(4271..4438)
                     /locus_tag="PKM27_11745"
                     /inference="COORDINATES: protein motif:HMM:NF021357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNR07981.1"
                     /translation="MVQNPNDLIFIISIPSEIKWITRDRPKIDRFACPWLITRFVDRE
                     AEFIYVPLDHY"
     gene            4705..5889
                     /locus_tag="PKM27_11750"
     CDS             4705..5889
                     /locus_tag="PKM27_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR07982.1"
                     /translation="MKYLALLYLTILCFYRPCAQNDSILLGFRHQFYSGLLQEQRTLS
                     VYLPASYSLPSYSETGYPLLFVLDGESLFHLASGIVHHLSNIIVNPPCPEMIVVGIHQ
                     IDRNQDLSPSDSASGKGQRFLEFIEKEVIPFIDKHYRTQSYRMLGGRSLGGLCVVNAL
                     VQLNQVDSYLAIEPSLWWDSQRFLDKSQQKLRTLDLRHKSLFLAIANTMDRGLDTLTV
                     QSDTAFNTLHIRSLLKFKSFIEAGEILPAGYKYYETENHQTVTPVALFDGLRYFFRDY
                     PITRTSQDYDDTSFSVAQKFKKHFDMVSVLYRMELKPAEADLKGLGLHFQSVSQFRKA
                     AELFLLNIQYYPRSPDAHATYAACLQAMGERTQAIEFYTKSLELKDQPEVRRKLEALI
                     KP"
     gene            6022..7203
                     /locus_tag="PKM27_11755"
     CDS             6022..7203
                     /locus_tag="PKM27_11755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR07983.1"
                     /translation="MPTRSKDVFLKLLDTTLNFSIDFIENGTTARAGFYREGPANFSI
                     KINDPGFYRAVLSYGNLGLGESFMKKRFEMTEGSLEGLIEALLACKLDRKLRHHLKLG
                     LKILPFRLLGSLRGKQSNVQKHYDAGLDLFSSFLDPSLTYSCGYAEKETDSLEQLQLN
                     KFERICRKLDLKPGERLLDIGCGFGGMLQYAATRYSVQGLGITNSEDHYQTGNDRIRK
                     AGLAHQIRIEKMDYTRATGIFHKIVSIGMLEHVPPAEYGRYFKTIHRLLDEHGKGLVH
                     FVGANAASNDHDYFIQKYIFPMSHQPRLSAIADTLERQGLAILDVENLIRHYGFTARQ
                     WLHNFRNNRSGLGRYPDSFLNMFEYYLACCVAAANASDSALYQVLFMKNHRGDIPLRR
                     V"
     gene            7354..9240
                     /locus_tag="PKM27_11760"
     CDS             7354..9240
                     /locus_tag="PKM27_11760"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR07984.1"
                     /translation="MFLLLAGRFNPTAAQNIIPLYDTLKACLDISDRVLVYEDKSGQL
                     KLEDILQRDSLFAKRDQPVPNYGVTRSVIWARLDIRPQAGEEFYLGLGFPNIDTVTLY
                     YPSGGKYAAKKTGWTFPNASRDLVAPDLVFKLPGQEGDTLLPYYLEARGRIVILPIAL
                     GTRTTLMESLHTNALYYLFYLGLVSMLFFYNLAIYITSRAKEYLFYSTWVFFATLYFM
                     ISKGYTNTMLPESFNALFRHVNIVSSLGGISILLFVQTTLRLKEYMPRVIKYYLALLA
                     LYLVIIFASLAKQFQLASNLSQLVLMLTVILGLWSGIVMYRRGHSFARYYMYGFVVTL
                     ISMTIYILTFQQVFPFNVFTNNSIVAGSGIEMILFSFGLGAKIKAINDEKQAAQELAI
                     SALKDKEQLIHEQNVMLETKVEERTQELKLEKKKSDDLLLNILPEEVAYELKENGSTE
                     AKNYAEVSVLFTDFVNFTGISEKLSPKELVSELHICFKGIDEIIEKNRMEKIKTIGDA
                     YLAVCGLPNRCETHALNCVHAGFQILEFMKKRKEEGGLFEIRIGINSGPVVAGIVGIK
                     KFAYDIWGDTVNTASRLQQNGAPGKINVSGSTYELIKSKYSCTYRGKLAAKNKGSIDM
                     YFVD"
     gene            complement(9275..10264)
                     /locus_tag="PKM27_11765"
     CDS             complement(9275..10264)
                     /locus_tag="PKM27_11765"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNR07985.1"
                     /translation="MRAFLFITSAFLLWTRLPAQLHWVQDSALQAGLPEGIRVFKNQG
                     SPDGKPFLAWYAQIDLRNKNLDLGADTTVGRRITPQKFYEQLDSPLLVINGTFFSFAT
                     QQNLNAVMHRGRLLAYNQHSVRGTREDSLYYYHPFRGALGFNKKRKPDVAWLYTDSTR
                     RYPYAFQMPRPALKDRNPGIKIHEVEPNINRWFAGNRRKGIRKRWKMETAIGGGPVLI
                     QDSQVRISSREERMFLNSEEVKHPRTAMGYTREHLIVLVIQGRFPGIAEGAGLSQVAN
                     ILQELGCMEAINLDGGGSSCMLINGKETIKPSDPTGQRPVPAVFYIRAKSNNR"
     gene            complement(10346..11725)
                     /locus_tag="PKM27_11770"
     CDS             complement(10346..11725)
                     /locus_tag="PKM27_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR07986.1"
                     /translation="MSVNRRKFLGASLLAGAGSALTAGNERGMPKPSPRRSANGKLAI
                     GILGVGGRGRSHVQQLLYREDCEVTAINDIDPKALEACNQLFVKAGKPLPKAYLKGDR
                     GYLDLLQNAGTDAIIIATPWEWHTEMAIESMKAGMYTATEVAGAFSLDECWELVNTHE
                     STGSHLFFLENVNYRRDVMAVLNMVRQQVFGELIHLECGYQHDLRDVKFNNGKQEYGG
                     GVEFNDRGFHEARWRTQHSVLRNGDLYPTHGIGPVAMMADINRGNRFVYLTSMASKGR
                     GLHDYIVNHPQGGPDHPNAKIEFKLGDVVTTMIKCNNGETITLFHDTNLPRPYSLGFR
                     VQGTKGLWMDVNQSLMIEGKTKSHEWTKADEWLRKFDHPLWKKYEKQAENAGHGGMDW
                     FVIHSFVEGAKTNAPAAIDVYDAASWLCITPLSEESIALGSHPVTFPDFTRGRWLKNK
                     NTFASGDLY"
     gene            complement(11890..12015)
                     /locus_tag="PKM27_11775"
     CDS             complement(11890..12015)
                     /locus_tag="PKM27_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07987.1"
                     /translation="MEESNVNYGKKGSCFGLFVFFVLLFILLLVWVYRLNGKLEF"
     gene            12232..13731
                     /gene="rpoN"
                     /locus_tag="PKM27_11780"
     CDS             12232..13731
                     /gene="rpoN"
                     /locus_tag="PKM27_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNR07988.1"
                     /translation="MLKQSISQKMLQKLSPQQIQLMKLLQIPSVNLEQRIKEELEINP
                     ALEEGDESGDVFDLEQNGDNLDEKATDEQNEKDEILDEYIMDYLEDDPSYYKMSTGPA
                     PAAETDKHSPMPVERTFHEYLMSQLSLLDLNERQLKIAVHIIGSIDDDGYLRRDPLAI
                     IDDLLFTQNIETSRKEILEVLKYIQQFDPPGVGARDLQECLVIQLRNKVSKNNAPDQQ
                     KRLECAHNILTSYFEEFTKKHFHKLERGLDLPEEELKEVIDEILKLNPKPASAFNETH
                     NPSVQYIIPDFIIINNEGVLELSLNSRNAPDLHISEHYRNLLNSYKETYKTNKTSRQA
                     KEAVLFIKQKIDSAKWFIDAIRQRQETMYRTMYAIMQYQQEYFKSGDERKLKPMILKD
                     IADISGYDISTVSRVANSKFVQTEFGTKRLKEFFSESLQTQEGEEVSTLEVKKIISDM
                     VGVENKKKPLSDEKIKTTLERKGYNIARRTIAKYREQLSIPVARLRKEL"
     gene            13774..14529
                     /locus_tag="PKM27_11785"
     CDS             13774..14529
                     /locus_tag="PKM27_11785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNR07989.1"
                     /translation="MLKKSHLFAFNAWVKGLVLFCRPHRFLGFTERLNLFFSNTVGLS
                     RWISLQAKSPFNDFYTPGRDGKKREQLYKQVILDQHLEGQPIDYLEFGVASGGSFFWW
                     LQENNHPDSRFYGFDTFEGLPEDWGLGFRKGDMSSGMPELNDQRAVFIKGLFQDSLPP
                     FLREYKNPGRKKIIHLDADLFSSTLFALASLSPFLKPGDILLFDEFNVPNHEYFAFKI
                     FSESFYIRTRLLGAVNNYHQVALRIEHNPASPV"
     gene            complement(14790..16088)
                     /locus_tag="PKM27_11790"
     CDS             complement(14790..16088)
                     /locus_tag="PKM27_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR07990.1"
                     /translation="MNQKPTLSFWQIWNMSFGFLGIQVGWGMQMGNMSAIYEYLGAKP
                     DEIPLLWLAAPMTGLLVQPIIGYMSDRTWGKWGRRQPYFTVGAILATLGLLAMPHSSA
                     LWMAAGLLWILDASINISMEPFRAFVGDMLPEHQINQGYTMQSMFIGIGNVIAALLPA
                     GILAVFGSGTHTGGVPPYLIAVFSIGALCYLSAVMYTVRSTREYPPADLEEFDRMRKE
                     TQGFRRGVKEVLSNIYNMPSTMRRVALVQFFTWPGLFLMWFYFTPAVARDILGAPDTT
                     SPLYAEGVAWGNICFGFYSVVCFLFSFYLPRIADRYSRKYTHMLCLAAGALGLLFVGV
                     APGKYWLLLSMTGVGIAWASILSMPYAMLAYTLPQQKLGVYMGIFNFFIVLPEILATL
                     FFGWIMEHVLDNNRIAAVMVGGGLLALAALLCLQIREQKA"
     gene            16252..17934
                     /locus_tag="PKM27_11795"
     CDS             16252..17934
                     /locus_tag="PKM27_11795"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HNR07991.1"
                     /translation="MNAVIGLDFGTDSVRALIVDSETGRELGSQVEYYPRWQKGLYCN
                     ASKNQWRQHPLDYMESMEAAVKAVIKQAGPEIARSIVGVGVDTTGSTPVAVDPEGTPL
                     SLLPGFEDNPNAMFILWKDHTAVQEAAAINRLCSDWETDYTKFEGGIYSSEWFWAKIL
                     RICREDEAVRKASWSWVEHCDWIPHLLTGGRDATLIKRSRCAAGHKALWHPSWGGLPP
                     QSFLTALDPVLGGLRDRLFTETHTSDEAVGHLSGEWATRLGLRTDVVVAVGAFDAHMG
                     AVGGQIEPYHLSKVMGTSTCDILVAPNEENEMLVKGICGQVDGSVIPGMLGLEAGQSA
                     FGDIYAWFRAVLAYPIHEIIGSSGLLDETTRGKLIRESFDRIIPELTKAAQKVPIGSG
                     GILSLDWMNGRRTPDANQYLKGVISGLNLGSDAPSIFRSLVESTCYGARLIVDRFRAE
                     GIPIKGLIGLGGVAKKSPFIMQVMADVMNMPIKIAASEQTCALGAAMFAAVAAGAQPD
                     LASAMRNMGSGFDAEYLPVPEHARLYEKYYREYCSLAGHMESHVMNEIHQNP"
     gene            17931..18644
                     /gene="araD"
                     /locus_tag="PKM27_11800"
     CDS             17931..18644
                     /gene="araD"
                     /locus_tag="PKM27_11800"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HNR07992.1"
                     /translation="MNKYDALKEEAFAANKQLPALGLVLFTFGNASAVDRREGVFAIK
                     PSGIAYEKLTPDQMVIVDFEARVVDGTLRPSSDTKTHAVLYKEWEGIGGIAHTHSTYA
                     TAWAQAVQDIPILGTTHADHLTTDIPCAPPMKDEYIHDDYEYMTGYQIVEELRERQWS
                     YKEVEMILVGSHAPFTWGTTVQKAVYNSAVLESIARMAWLTLQIRPGVPSMKDTLIQK
                     HYERKHGEGRYYGNEEFKF"
     gene            18820..19326
                     /locus_tag="PKM27_11805"
     CDS             18820..19326
                     /locus_tag="PKM27_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07993.1"
                     /translation="MKVKIIRLSDHQEVVARIDFPKGIPLPSITDGWKFNFKSHSKKR
                     GLITYILVCEDTPNKIEGCIIFQLKDAIEPYMAYIEIAPHNKGEKKHFDRVAGCLIAY
                     ACRLSFIHGKGPYKGWLTFDVLEEDKQDEIKLMAVYCKKYGAQKFGDTTMVISPEAGE
                     NLINKYLN"
     gene            19333..19806
                     /locus_tag="PKM27_11810"
     CDS             19333..19806
                     /locus_tag="PKM27_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR07994.1"
                     /translation="MKRNEVRITDLWNKEKSKSLSEFIIDQSNKQTKERKLRNELLSI
                     QFKIEDYIESENVIQKLNILDFVKMYLSALNITQKRLADLFEMKDSNLYKYLVGERKL
                     NTTIVLKLSSFTDLNPEYWLRIEVKNELIEINKEKIKIKFFRKYNYQNLLTVQED"
     gene            20030..21754
                     /locus_tag="PKM27_11815"
     CDS             20030..21754
                     /locus_tag="PKM27_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNR07995.1"
                     /translation="MNSKILLIAQLFLISTGLPAQTFGIHELNQKLGKGINMGNMFEA
                     PAEGEWGNPFRDDYFQKIAGLGFNHVRIPIRWDVAARALQTAPFTIQPSFLERIKYVV
                     DLALSQGLYVIINMHHHEALFQDPEGVKPRFLSQWGQISDYFKNYDQRLLFEVLNEPN
                     TNLTGQKWNQFFKDALGEIRKTNPDRAVLMGIAPWGGLGGVPDLVVPEDENIIITVHY
                     YDPFTFTHQGAEWVDNSTPWLGTKWENTTLERNEITGSFAYLINFAKSINKPIHVGEF
                     GAYSKADLNSRVLWTNFLARWFESQGFSWAYWEWSAGFGIFDPFTNQYLTRLADALLK
                     DPMLPAKPQTTVKVFESGFGPGDGWNLYVQGGAAASLSRTGGQGIVDVTQTSGTGWHV
                     QLVQNNIQLKKDSRYLVTVKASSNTPVSITNYLGMNFSPWGSYSGYKSLSLNTAETEQ
                     TYSFIMTSPTDPAARLVFDLGNKPSRITISQVKVEEVLDVPVSYKQTISHPIKVFPNP
                     SAARIRIEGIDQPGRLQILSADGKLCINFGPWNPMNTVDVAELIPGIYFLRIVNSRGI
                     FNTPFIKY"
     gene            21951..23453
                     /gene="araA"
                     /locus_tag="PKM27_11820"
     CDS             21951..23453
                     /gene="araA"
                     /locus_tag="PKM27_11820"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HNR07996.1"
                     /translation="MIKSLTEFEVWLVTGSQHLYGPETLAQVGHHVKQIAHFLDQQGP
                     IPVQVISKPVVKSPEEIYQVVKEANASDFCIGLICWMHTFSPAKMWIRGLKALQKPMV
                     HLHTQFNEQIPWSAIDMDFMNLHQSAHGDREFGFIGSALKLERKVIVGHWKEEEVIRK
                     LGRWTRVAAAWADSQTMKIARFGDNMRQVAVTEGNKVSAQVRFGYEVSGYGLGDLVQY
                     IDSVTESEVIHTIQEYEDHYTLMQSLGHGGEKRESLFEAARIEAGLRHFLHDGGFTAF
                     TDTFEDLHGLSQLPGIAVQRLMHEGYGFAAEGDWKTAALVRTMKVMALGLEGGNSFME
                     DYTYHFDLQGSRVLGAHMLEICPSITRQKPRCEIHPLSIGGKEDPVRLVFDGAPGKAL
                     NASMIDLGHRFRLVVNQVEAVDVGHEMPLLPVARVLWDSKPGLAASAEAWILAGGAHH
                     TCFSQNITIEDLEDYASMAGIEIVVIDENLPPLRDYKQMLRNNEIYYGLR"
     gene            23654..24523
                     /locus_tag="PKM27_11825"
     CDS             23654..24523
                     /locus_tag="PKM27_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNR07997.1"
                     /translation="MDHFVFIINKSSGRQKSGELESRIMAAVKDRPMSAECHITQNAE
                     HTAELVQTALARGRENFVAVGGDGTVNTLARHLVHRKAILGIIPRGSGNGLARHLGMP
                     ANINDAIERLLPRRTQSVDAILINDHWSFNVAGIGFDGYISSLFGQNGKRGVANYIRL
                     INQEYKSYQPIEMEIHTDGRILHEQVFQLAIANASQYGNRAIIAPFAALDDQLLDISM
                     ISKVPYTFLPGFFLRVIAGKIHKSRYWNSFKTNRMYVKTSRPVHFHTDGDGRGISEHF
                     NVELVPSCLKLIY"
     gene            complement(24507..>24705)
                     /locus_tag="PKM27_11830"
     CDS             complement(24507..>24705)
                     /locus_tag="PKM27_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07998.1"
                     /translation="ALSDCHLLRSRLTLSDNHLLALSDCHLLRSRLTLSDNRLLALSD
                     CHLLGLGLALNDNHFAINRSV"
     assembly_gap    24706..24805
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24983..25846)
                     /locus_tag="PKM27_11835"
     CDS             complement(24983..25846)
                     /locus_tag="PKM27_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR07999.1"
                     /translation="MNIAFVTDREHDDLTPDDRLLVNWFAKQGHRVIAAVWDDPDIKW
                     DPFDAIVIRSVWDYFKRIHEFDRWLEKMETVKKPIFNPLSVVRWNKNKTYLGWFKEKG
                     ILIPEGIIRPQGHSADLKGWLEQLGFEKAVIKPTISGGAYKTWVTTPDLANRHQTAFD
                     ALLNEQDVILQRFSEEIVSHGELSLIYFNRKFSHAICKKAKPGDFRVQTQYGGQYHPY
                     FPEYELFLQVEKILELIPETLLYARIDGYLDACNRFVLMELELIEPALFLDSHPDACR
                     NFYEAFTAMLI"
     gene            complement(25858..27051)
                     /locus_tag="PKM27_11840"
     CDS             complement(25858..27051)
                     /locus_tag="PKM27_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR08000.1"
                     /translation="MPLFHASLIYTSEHPDPLEGHVLETDDKGKILNILPLERVEDRK
                     TQIQFIEGILVPGFVNAHCHLELSHMKNMIPTGTGLVEFIKNIVRRRKEVDRQGILEA
                     IAAGEQEMIAGGIVAVGDISNVEDTFYQKSRGLLRYHSFVEAFDLLQDEKAEAEMARA
                     YEVWSKLHCPPGHKKSLVPHAPYSVSSSLFELLRTHPHEGESISIHHQETHAENHFFL
                     EKSGALIDFYQTLGVSLEAFEATRAPSSLQVIQNMSAGRRTLLVHNTISTPRDIRSVE
                     EWNEEVYWVTCPNANLYIENQLPRYLHFIEHGAKMCVGTDSLASNWQLSILEELKTIQ
                     HYQSYLTFDRLIRWATLNGALALGFEKDLGSFSPGKTPGVVQIHPFDLSRRQLMPGSR
                     ANRLI"
BASE COUNT         7147 a         7015 c         6531 g         6454 t
ORIGIN      
        1 ccctgtcaat ctttattacg aacaaattcg tattttatga aaatattaac ccttttccgg
       61 cccggccaaa aagcaaccgc ccggccgggg atttgtcttt atttataagt taaatgtccg
      121 gttaaaacca atattggtct aaatcgccgg cgttatcatg gttaaaagaa taatatttga
      181 aaggtcctct acttcattaa acaggtcagg gatgatgaat aaaatattat ggcatgccgt
      241 atccatcagc gccctcgtca gaacgaggcc tgaaaacaat gccccggtgg tgaaatcggt
      301 ctccaacggg atctggctcg gcgtcatggc cgagatgggg gaatggatct atgtcgtcgg
      361 caaggaagcc attggctggg tgcttcggaa agagattacg acctgcgacg cccaggccct
      421 gcatatccgg caggatgagg caggatgggt ggttgggtat tagtagtcaa tagtcaacag
      481 tcaatagtca atagtcaata gtcaacagtc aatagcaagt tagcaggtgt agctcgcttt
      541 tcgtttctgg cttctcgttc cttgatcagt gcggcaggcc cctttcaggg gtcaggggtg
      601 tgcggatgaa aaaagaaatc agtggtcagg aatcattagt cagatagcag gtctagctcg
      661 cttctcgttt ctggcttctc gcttctggct tctggcttct cgtttctcgc ttctcgcttc
      721 tcgtttctgg cttctcgctt ctcgcttctc gcttctcgct tctcgcttcc cgcttctcgc
      781 ttgctaatac attttcaacc ccttttggta ctatccaatc taatcaacca tcccgggcta
      841 aaattatgcc ccaaactata aaccgctccg cgaaaaccga ttaattttat tcattcctag
      901 ccctatctac caggctggat gatttaaatt aatggagtat gaatcgcaga aataccataa
      961 aacatttatt cgcggcctcc ggcgccctca ccgcccttcc ttcctgggcc aggggctgga
     1021 cggcatcatc ctttaaaact tttcgttctt ccttcaacaa ggcggaacag gaaatcctgt
     1081 ccgcggtcgt ggataccatc attcccgcag ggaatgcgat cggtgcaaaa tcggtcggcg
     1141 tggacctttt cctccaaaag ctctttgacc gctgttatga aaaggaagtg caggacaatg
     1201 taaaaaaaca gttgcaagcc ctggaatcca aagcccaggc cggccatcaa acctcctacg
     1261 cggcctgccc ctctgctctg cgggaacagt tgctcctgca attcgcgtct tccgaaatca
     1321 aggaagaaaa ggattttttc accctgatca agtccgagac catccgcgga ttcaacactt
     1381 cggaacaggt gatggtccaa tacctgaagt acaaagtggc cccgggacat tattacggat
     1441 gtgtcgacgt caacgcctaa ataataaagg cacctgattt aaaataaaat cttctttgcg
     1501 cctttgcgcc tctgcgagaa acaaaaaaca tcatgacaaa cttcaacata gattcagcta
     1561 aaaaacgcag ttacgacgcc atcgtcatcg gttccggagc ctcaggcggc tgggccgcca
     1621 aggaattgtg cgatcatggc ctcaaaaccc tggtcctcga acggggacgg gacgtaaaac
     1681 atatcgtaga ctatcccacc gcgtccaaag ccccctggga attcgaattc cggggcacgg
     1741 tcccgctcga aaaaagggcc ggataccagt ttggccgttt tatgcaggaa gccaccctgc
     1801 actgggcact gaaagacgac gaacaacccg tggtctatga aaaacccttt cgctggttca
     1861 ggggatacca tgtgggcggc aaatccctgc tttgggcccg ccagacgcaa cgctggagcg
     1921 attttgattt tgaagggccg gcccgcgatg gatttgctgt tgactggccg atccggtata
     1981 aagaccttgc gccctggtac agccatgtgg aaaaattcgc agggatcgcc ggaaacaaag
     2041 acggcattgc ggaattgccg gacagcgaag tcatgcccgg cttcgaaatg tcctgcatcg
     2101 aacaatattt taaggaaaaa ctaaaggctc atttcccaga ccgccacctc atccaggccc
     2161 gctgcgccca catcaccgac ccccaggaaa tacatatcca gcagggccgc agcaaatgcc
     2221 aaaaccaggc catgtgcaac cggggctgca tctacggcgc ttatttcagc agcaatgcct
     2281 ccaccatccc ctgggccctg aaaaccaaaa agctgacgat gcggcccgac tccgtggtcc
     2341 attccatcat ctacgatgaa aagaaaggcc gcgccacagg ggtccgcgtc atcgaccggc
     2401 acagccatga aatgatcgag ttccacgcaa agatcatttt tgtcaatgcc tccacgatca
     2461 acagcaccgc catcctcctg aattccaaat ccaaccgttt ccctaacggg ctgggcaacg
     2521 acaacggcct cctgggcaaa ttcctttcct ggcacaatta caggggcaag gccaatgccc
     2581 agtttgaagg cttcgcggac aaacgcaccg acggccgcaa cccttccaac agttatatcc
     2641 cccggtttcg gaatgtgcgg aaacaggaaa tggacttcct ccgcgggtac gccatcggca
     2701 ttgggggtgg cagggggacc agcaccgata cttccatgat cggcgaccag ctccgtgaaa
     2761 acatcctgaa tcccaaatac gggcgctgga atatgagctc ctggatgatg ggcgaatgtg
     2821 tccccatcga atccaaccac atgcgcctcc accatgaact gaaagacaaa tacggcatac
     2881 cccagatcgt gctttcctgt gaatggaccg aaaacgacga caagatggtg gccgactatg
     2941 tggagcaaag caaagccatg tttgaagccg cgggatttat caacattacg gccaacgata
     3001 ccaaatccgc ccccggctcc gacatccacg aaatgggtgg ggtgcgcatg ggtcatgatc
     3061 ccaaaacttc cctgctcaac ggctggaacc agatgcatca ttgcaaaaat gtcttggtca
     3121 gtgacggcgc ctgcatgacc tcgacgggta ctcagaatcc taccttgacc ttcatggcga
     3181 tcacggcgag ggctgcgaat catgcggtgg aggagctgaa gaggaggaat atttaagttt
     3241 gtctgggcag gacgaaagtt gaacggggga cctatttaag gtctttcgcg caggccctaa
     3301 tattactttc aagccgcttc aacatttcca gcctcgagca gatcttgacc aggcgttgta
     3361 agacctgccc ctatccccaa taaataaatt tccattcggg acgtcctcag catcttctca
     3421 taatcataat ccattttgag atgatcctga attgaaaaca tttcgtcaca gtaggtccgt
     3481 aagtcatttt caaccatttc gaagctggct tacgaaccca tgggtatgct taaccttgtg
     3541 ataagatttt atccccattt gacggctcgg ggattgacca aatcaaggga gaactaaaaa
     3601 caagttctaa agaagggtga ggtggttaac tgccttctta atgaaatata tgatcatggg
     3661 caagtcccta caacaggagg tcagaggatg catcgccccc catatgagga atggaattgt
     3721 aaataagata aaatcagtta cgaataaaag gtataacact aattaatttt acagatatga
     3781 actcaattaa attagcactt gcacttttgc tcgcaccctt atttttccaa atggctaacg
     3841 ggcaggaaac cggtcaaatg gtaaggctgg ccaaactggt gatcgattcg actcagttgg
     3901 aagcctatca ggtcatttta aaggaagaaa tagccacctc tgtaaaatta gagcctggcg
     3961 ttttgacctt atatgccgtt gccgaaaaag caaatcccaa tcatatcacc atacttgaaa
     4021 tatactccgg tattgaggct tatgaaaaac atatccaaac cccccatttt ctgaagtaca
     4081 aagacggcac aaaacacatg gtgaagtcac ttgaattgat tgcaaccatt cccctcattc
     4141 cagaaatgaa aattaagtaa cggcaaagct tgtcaagcaa agttcccgtc caggtcagta
     4201 agattgtcaa tatgcatttg tccaggctct gtgcaaaaaa cactggatga aggcaataaa
     4261 gttgtcgcgt tcagtaatga tccagaggca cataaataaa ttccgcttcc cggtctacaa
     4321 atcgtgtaat cagccagggg caggcgaagc ggtcgatttt cgggcgatca cgggtgatcc
     4381 attttatctc cgaagggata cttatgataa atataaggtc attcgggttt tgaaccataa
     4441 agacagggaa gcactgttct ccgcccccgt tacccgcctg atttgagcga ccgggaaccc
     4501 tgtaaacagc ttagggaagt cctgaaggat aaaattttca aactcccgct tcatcttgcc
     4561 cggcggtaga gagcgtcctt gttcttcagc taattgaaat ccccttgatc ggtatgctcc
     4621 ttaagattgt tcccggtcca cttgaggtcc ttttaatggg ctgcccgata aaattcctat
     4681 ttttaatttt ataaattagg cttagtgaaa tacctcgccc tgctttatct gaccatcctt
     4741 tgcttttacc ggccctgcgc tcagaacgat tccatcctgc tgggattccg ccaccaattc
     4801 tattccggcc tgttgcagga gcaacgcact ttatccgtgt acctgcctgc cagttattct
     4861 cttccatcct attctgaaac aggctacccc ttgctttttg tcctggacgg ggaaagcctt
     4921 ttccacctgg cttcaggcat agtccatcat ctcagtaata tcattgtaaa ccccccttgt
     4981 ccggaaatga tcgtagtggg cattcaccag atcgaccgca accaggacct gagtccctct
     5041 gactctgcaa gcggtaaagg ccaacggttt ctggaattta tagaaaagga ggtcattcca
     5101 tttatcgaca agcattatcg cacccagtcc taccggatgc taggcggaag gtccctcggc
     5161 gggctctgtg tggtcaacgc actcgttcaa ctcaaccagg tggattccta cttggccatc
     5221 gagcccagcc tgtggtggga ttctcaacgc ttcctggaca agagccagca aaaattgagg
     5281 actctggacc tccgtcacaa aagcctcttc ctggctattg caaataccat ggaccggggc
     5341 ctggataccc tgaccgttca atccgacacg gcctttaaca ccctgcacat ccgctccctt
     5401 ttgaaattta aaagttttat tgaagcgggt gaaattctgc ctgcaggata caaatattat
     5461 gaaacagaaa atcaccaaac cgtgaccccg gtcgccctct ttgatggtct ccgatatttt
     5521 ttccgcgatt atcccatcac tcggacgagc caggattatg acgataccag tttttctgtc
     5581 gcccaaaaat tcaaaaaaca ttttgatatg gtttcggttc tctaccgtat ggaactgaaa
     5641 cccgcagagg ccgatctgaa aggtcttgga cttcattttc agagcgtgtc ccagttccgt
     5701 aaagcggccg aattgttttt gctcaacatt caatattatc caaggagtcc cgatgcgcac
     5761 gccacctatg ccgcttgcct gcaggccatg ggtgaaagga cccaggccat tgaattttat
     5821 accaaatctc tggaattaaa agatcagccg gaagttcgca gaaaactgga agcactgatc
     5881 aaaccatgac atggaataca ttctgcgatc gcgaatccaa ggatgcggat tttattccaa
     5941 ttttaaatta ttttaatttt aggctccaac caggaagtag agccccccgg ctcccgttaa
     6001 tccggtctaa tcttaaaagc catgccaacc cggtcgaaag acgtgttttt gaaattgctg
     6061 gataccaccc ttaatttctc catagacttc atcgagaatg gaaccacggc cagggcaggt
     6121 ttctaccgcg aaggccctgc aaatttttcc attaaaatca acgaccccgg tttttaccgc
     6181 gcggtgctat cctacggtaa tctcggactg ggcgaatcct ttatgaaaaa gagattcgaa
     6241 atgacggaag gttccctgga aggtctgatc gaggccctgc tggcctgtaa actggaccgt
     6301 aaactcagac accacctgaa actggggctt aagattttac ccttccgcct acttggatcc
     6361 ttgcggggaa aacaatccaa tgtacagaag cattacgacg cgggcctgga cctgttcagt
     6421 tccttcctcg atccttccct cacctattcc tgcggctatg ccgagaaaga aaccgacagc
     6481 ctcgaacaat tgcagctcaa caaatttgaa cgcatctgcc ggaaactcga tctaaaaccc
     6541 ggtgaacggc tgctggatat aggatgtggt tttgggggca tgcttcagta cgccgctacc
     6601 cgctattcgg tccagggcct ggggatcacc aacagcgagg atcattatca aaccgggaac
     6661 gaccggatcc ggaaggcagg gcttgcccac cagatcagaa tagaaaaaat ggattacacc
     6721 cgggcaacgg gtattttcca caaaatcgta agcatcggca tgctggaaca tgtgccgccg
     6781 gcagaatacg gtcgatattt caaaaccatt catcgtttgc tcgatgaaca cggaaagggc
     6841 ctggtccact ttgtcggcgc caacgcggct tccaacgacc acgattattt catacagaaa
     6901 tacatcttcc ccatgtccca tcaaccacgg ctgtccgcga tcgcggacac cctggaacgc
     6961 cagggcctgg ccatcctgga cgtggaaaac ctgatccgcc attacggatt cacggcccgc
     7021 cagtggcttc ataatttccg gaacaaccgg tctggcctgg gccggtatcc cgattcgttt
     7081 ctcaatatgt ttgaatatta cctggcctgt tgcgtcgccg cggcgaatgc ctcggactcg
     7141 gccctttacc aggtattatt tatgaaaaat catagggggg acatcccgct caggagggtc
     7201 tgagcgccgg ccggtgatca cagaagcatt cccaatggca ggaatacaga agataaacgc
     7261 cggcaccaga ccttattcct tttacgccgg agaactcagg tttgtctaaa ccgctatctt
     7321 tgaggcccga tgggccggaa aactttgttt ttcatgtttc tgcttcttgc cggccggttt
     7381 aaccccactg ccgcgcaaaa catcataccc ttatatgata ccctgaaggc ctgcctggac
     7441 atcagcgacc gggtcctggt gtatgaagac aaaagcggcc aactgaagct ggaagatatt
     7501 ttgcagaggg attccctctt cgcgaaaagg gatcaacccg ttcccaatta tggggtcact
     7561 cgctctgtca tctgggcccg gctggatatc cgtccccaag ccggagagga attttatctc
     7621 ggcctgggat tcccgaacat cgacacggtc accctatatt atccttccgg gggaaaatac
     7681 gccgcaaaaa aaacgggatg gaccttcccc aacgcatcac gggacctggt ggcccctgac
     7741 cttgttttca aattgccggg ccaggaaggg gataccctcc tgccctatta cctggaagcg
     7801 cgcggccgga tcgttatcct gcccatcgcg ctggggaccc ggaccaccct tatggaaagc
     7861 ctgcatacca acgcgctgta ttatcttttt tatctcggcc tggtcagcat gctgtttttt
     7921 tataacctgg ccatctacat cacttcgcgc gcaaaggaat atttgtttta ctccacctgg
     7981 gttttcttcg ccacccttta ctttatgatc tccaaagggt acaccaatac catgcttccg
     8041 gaatccttta atgccttgtt ccggcatgtc aatatcgtat cctccctcgg cggcatcagc
     8101 attctcctgt ttgtccagac caccctgagg ctgaaagaat acatgccccg cgtgataaaa
     8161 tattacctcg ccctgctggc cctctacctg gtcatcatct ttgcctccct ggcgaaacaa
     8221 ttccagctgg cctccaacct gtcccagctg gtcctgatgc tgacggtgat cctgggactt
     8281 tggtcgggga tcgtgatgta ccggaggggc cattcctttg cccggtatta catgtacggt
     8341 tttgtagtga ccctgatttc catgaccatt tatatcctga ccttccagca ggtcttcccc
     8401 tttaacgtgt ttaccaataa ttccatcgtg gccggcagcg ggattgagat gatcttgttt
     8461 tccttcggcc tcggggcaaa aataaaagcc attaacgacg aaaagcaggc ggcccaggaa
     8521 ctggccattt ccgccctcaa ggataaggaa caactgatcc atgaacaaaa cgtcatgctg
     8581 gaaacgaaag tcgaggagcg tacccaggaa ctcaaactgg aaaagaaaaa atccgatgac
     8641 ctgctgctca atatcctgcc ggaagaagtg gcttacgaac tgaaggaaaa cggaagcacg
     8701 gaagctaaaa actacgccga ggtcagtgtc ctgtttaccg attttgtcaa ttttaccggc
     8761 atcagcgaaa agctctcccc gaaagaactt gtcagtgaac tgcatatctg cttcaaaggc
     8821 attgacgaga tcattgaaaa aaacaggatg gaaaaaataa aaaccatcgg ggatgcctac
     8881 ctggcggtct gcggattgcc caaccgctgt gaaacccatg cgctcaattg cgtacacgcc
     8941 ggctttcaga tcctggaatt tatgaaaaaa agaaaggaag aaggcggcct gtttgaaatc
     9001 cgcatcggca tcaacagcgg tccggtcgtg gccgggattg tcggtattaa aaaattcgct
     9061 tacgacatct ggggggacac cgtcaacacc gcctcgcgcc ttcagcaaaa cggagctccc
     9121 ggaaaaatca atgtcagcgg cagcacctat gaactcatca aatcaaaata ttcctgcacg
     9181 taccggggca aactggccgc aaaaaacaag ggcagcatcg atatgtattt tgtggattaa
     9241 taaaacccgg tgttccccgt ataccagcac cggatcatct gttgttcgat tttgccctta
     9301 tataaaacac cgcaggcacc ggcctttgcc ctgtgggatc ggaaggtttg atggtttcct
     9361 ttccgttgat gagcatgcaa ctgctgcccc cgccatccag gttgatagcc tccatgcaac
     9421 ccagttcctg cagtatgttg gccacctggc tgagtccggc gccctcagca atgcccggga
     9481 atcgcccctg gatgaccagt acgatcaggt gctcccgggt ataacccatg gccgtcctcg
     9541 gatgttttac ttcctccgaa ttcaggaaca tccgctcttc ccggctgctg atgcgcactt
     9601 ggctatcctg gatcaatacc gggcccccgc ctatcgctgt ctccatcttc catctcttcc
     9661 ggattccctt cctccggttg ccggcaaacc agcgattgat attgggctcg acctcatgga
     9721 tttttattcc cggattgcgg tcttttaagg cgggcctggg catttggaag gcataggggt
     9781 acctccttgt agaatccgtg tagagccacg cgacatccgg ttttcttttt ttattaaacc
     9841 ccagggcgcc ccggaaaggg tgataataat aaagggaatc ctcccgggta ccccggacac
     9901 tgtgttggtt ataggccagc aggcgacccc tgtgcatcac ggcattgaga ttctgttggg
     9961 tggcaaagga aaaaaaagtg ccgttgatca cgagcagagg agagtccagt tgctcataaa
    10021 atttttgagg cgtgatccgc cggccaaccg tagtatctgc tcccaggtca agattcttat
    10081 tccgtaaatc tatctgggca taccaggcca ggaagggttt tccatccgga ctaccctggt
    10141 tcttaaaaac acggatgcct tcaggcagtc cggcctgcag ggcagaatcc tgaacccaat
    10201 gcagttgtgc gggaagccgg gtccacagca aaaaagcgct cgtaataaac agaaatgccc
    10261 gcataaacat attttataaa gtatatatat gtaaatttcc tatacgtggc gtttcaataa
    10321 ccttcaacag ctattgcgac tgtattcaat acaggtctcc tgaggcaaat gtatttttat
    10381 ttttcaacca cctgcccctg gtaaaatccg gaaaggtaac cggatggctg cccaaggcaa
    10441 tgctttcctc cgaaaggggg gtgatgcaga gccaggaggc ggcatcgtac acatcgatgg
    10501 cggccggtgc attggttttg gcgccttcca caaaagagtg gatcacaaac cagtccatac
    10561 cgccgtgccc ggcattttca gcctgttttt catatttttt ccaaaggggg tgatcaaact
    10621 tccggagcca ctcgtcggcc tttgtccatt catgggattt tgtttttccc tcgatcatca
    10681 gcgactggtt gacgtccatc cacaaaccct tggtaccctg gacccggaat cccagggaat
    10741 aaggcctagg caggttggta tcgtgaaaaa gggtgatggt ctccccgttg ttgcacttga
    10801 tcatagtagt cactacgtcg cccagcttaa actcgatttt ggcattggga tggtccggac
    10861 cgccctgggg atgatttaca atataatcgt gaaggcccct gccctttgaa gccattgaag
    10921 tgaggtaaac aaaacggttg ccccggttga tgtcggccat catggccacg ggtccaatgc
    10981 catgggtggg atacaggtcc ccgtttcgca gcacggaatg ttgggtgcgc cagcgggcct
    11041 cgtgaaatcc ccggtcatta aattccaccc ctcctccgta ttcctgtttc ccgttgttga
    11101 acttgacatc ccggagatcg tgctgatagc cgcattccag gtggatcagt tccccaaaaa
    11161 cctgttgacg gaccatgttc agcacggcca tgacatcgcg gcggtaattt acattttcca
    11221 ggaaaaacaa atgactgcct gtggattcgt gggtattgac cagctcccag cattcatcca
    11281 gggaaaatgc cccggcgacc tcagtggccg tatacattcc ggctttcatg ctctcgatgg
    11341 ccatttcggt atgccactcc cagggtgtgg cgatgatgat ggcatcggtg ccggcatttt
    11401 gcaaaaggtc aaggtaaccg cggtcccctt tgagataggc tttgggaagt ggttttcccg
    11461 ctttgacaaa caactgattg caggcttcaa gagctttggg gtcaatgtcg ttgatcgcag
    11521 tgacttcaca atcctccctg taaagcaatt gctgcacatg gctgcggcca cggcccccga
    11581 cacccaggat gcctatggcc agtttaccat tggcggaacg ccggggactt ggttttggca
    11641 tccccctttc atttccggcg gtgagcgcag atcccgctcc ggccaggagg gaagccccta
    11701 aaaatttgcg acggttgact gacatatgga tttaatggat ggttgattag cttatgatcc
    11761 gcacggagcc gctgcccccc ggggtccggc ccactaatgt aaagaaagga accaggaatt
    11821 tcgcaggcaa gaacttcttt tccttaaaac cgaactaaag gggaatccct ctccagcctt
    11881 gccgggatat taaaactcca gcttgccgtt gagccggtaa acccaaacca gtaaaaggat
    11941 aaacaagagc acaaaaaaca cgaacaggcc aaaacaggag ccttttttac cataatttac
    12001 gtttgattct tccatgaccg tctgctgaaa tagtacacaa agaaacatca attttctgat
    12061 ctttaataag cggttctaca gaaaatacag attcccctat atttacatat aaaatattaa
    12121 tatataattt aataaatatt aaaattaaac cacatatata taggaggtct gttatctttg
    12181 ttccgggatg aacctattgg tccttttatg tgtaatacaa attaataaac gatgttaaag
    12241 cagtcgataa gtcagaagat gctccaaaag ctgtctcccc agcagataca gctcatgaag
    12301 ttgctgcaga tacccagcgt caacctggaa cagaggatca aggaagaact ggaaatcaat
    12361 ccggctctgg aggaagggga cgaatcgggg gatgtatttg acctggagca aaacggggac
    12421 aatctggatg aaaaagccac ggacgaacaa aatgaaaagg atgagatcct cgatgaatac
    12481 atcatggatt acctggagga tgatccctct tattataaaa tgagcaccgg gccggctccg
    12541 gcggccgaga cggacaaaca ttcccccatg ccggtagaac ggaccttcca tgaatacctg
    12601 atgagccagc tgagcctgct tgacctgaat gaaagacaat taaaaattgc cgtgcacatc
    12661 attggcagca tcgatgatga cggatacctt cgccgggatc ccctggccat catcgacgat
    12721 ttgttattta cccaaaacat tgagaccagc cgcaaggaaa tcctggaagt cctcaaatac
    12781 atccagcagt ttgatccccc cggggtcggc gccagggatt tgcaggaatg tctggtgatc
    12841 cagcttcgca acaaagtcag taagaataat gcgccggacc agcaaaaaag actggaatgc
    12901 gcccacaaca tcctgacttc ctattttgaa gaattcacca aaaaacattt ccacaaactg
    12961 gagcgtgggc tggaccttcc ggaggaagaa ctcaaagaag tgatcgacga gatcctgaag
    13021 ctgaacccca aacccgccag cgcttttaac gagactcaca acccctcggt ccagtacatc
    13081 atccctgatt ttattatcat caacaacgaa ggggtcctcg aactttccct gaattccagg
    13141 aacgccccgg acctccatat cagcgagcat taccgcaacc tcctgaattc ctacaaagaa
    13201 acctacaaga ccaacaagac gtcccgccag gccaaagaag cggtgctttt tatcaaacaa
    13261 aaaatcgaca gcgccaaatg gtttatcgat gccatccggc agcgccagga gaccatgtac
    13321 cggaccatgt atgccatcat gcaataccag caggaatatt tcaagtcggg agatgaacgg
    13381 aaactcaaac ctatgatcct gaaagacatc gcggacatct cgggttatga tatttccacc
    13441 gtttccaggg tggccaacag taaatttgtg cagaccgaat tcggaaccaa aaggctgaaa
    13501 gagtttttct ccgaatccct ccagacccag gaaggggaag aggtctctac cctcgaagtc
    13561 aaaaaaatca tttccgatat ggtcggggtg gaaaacaaaa agaaacccct ttcagatgaa
    13621 aaaataaaaa ccaccctcga gcgcaaaggc tacaacatcg cccgcaggac tattgccaaa
    13681 taccgggaac agctgagcat ccccgtagcc cggctgcgta aggaattgta accctttgtt
    13741 ttagcaggac tggaaaacca aacggttgtc cccatgttaa aaaaatctca tttattcgcc
    13801 ttcaacgcct gggtcaaagg cctggtctta ttttgccggc cccatcgatt cctgggcttc
    13861 acggagcgac tgaatttatt tttttccaat acggtcggct tgagcaggtg gatttccctt
    13921 caggcaaaaa gtccattcaa tgatttttac acccccggga gggacggtaa gaaaagggaa
    13981 cagctttaca aacaggtcat tctcgatcag cacctggagg gtcaaccgat agattatctt
    14041 gaattcggcg ttgccagcgg cggttccttt ttctggtggc tgcaggagaa taaccacccg
    14101 gattcccgtt tttacggctt cgacaccttt gaaggattgc ctgaagactg gggtttggga
    14161 ttccggaaag gcgatatgtc ttcgggtatg cctgaactga acgatcagag ggcggtgttt
    14221 ataaagggcc ttttccagga cagcctgccg cccttcctca gggaatataa aaacccgggc
    14281 aggaaaaaaa tcattcatct tgatgccgat ttgttttcat cgactttatt tgctctggcc
    14341 agcctgtctc cctttttgaa gccgggagat attctccttt ttgatgaatt caatgtgccc
    14401 aaccacgaat attttgcttt caaaatcttt tctgagtcat tctatatccg gacccgtttg
    14461 ttgggagcgg tcaacaacta tcaccaggtc gccctccgga ttgagcacaa tccagcttcc
    14521 ccggtttgat ctatcctcca ttcccaatgg tatgcgagga tcaatactcc gagcttttga
    14581 ggattaaggg ggagtcaccc attagtcagt gggcttaaat gggcactgtc aattcttcaa
    14641 tacttgttca acgagaaacg ttcggaaagc gtcccggaca agagtctttg tgcaaccttg
    14701 atgatctgaa cgcaggccga agtcaataga aacccaggcg aaggttccga aagttcaaca
    14761 gatccgggaa agtatttgat tttacaggat caggcttttt gttcccggat ctgcaaacaa
    14821 agcagggccg caagggccag caatccacct ccgaccatga cggcggcaat ccggttgttg
    14881 tccagcacat gttccataat ccagccgaaa aacaaggttg ccaggatctc cggcaggacg
    14941 ataaaaaaat tgaagatgcc catgtatacg cccagtttct gctggggaag ggtataagcc
    15001 agcatggcgt atggcatgga aaggatgctg gcccaggcaa tccccacccc ggtcatggaa
    15061 agcaacagcc agtacttccc cggcgcgacg cccacgaata acaaacccag ggcgccggcg
    15121 gccaggcaga gcatatgggt atattttcgc gaataccggt cggctatcct gggcaggtaa
    15181 aaagaaaaca gaaaacagac caccgaataa aacccaaaac agatatttcc ccaggccacc
    15241 ccttcggcat ataaggggga ggtggtatcc ggcgcgccca ggatatcgcg ggcgacggcc
    15301 ggtgtaaaat aaaaccacat gaggaataat cccggccagg taaaaaactg gaccagcgcc
    15361 acgcgccgca tcgtcgaggg catattataa atattggaaa ggacttcctt gaccccccgg
    15421 cggaatccct gggtttcctt tctcatccgg tcaaactcct ccaggtcggc cggcggatat
    15481 tccctggtcg agcgcaccgt atacatcact gcgcttaaat aacacaaggc cccaatggaa
    15541 aaaaccgcga tcagataagg cggcactccc ccggtatggg tgccgctgcc aaatacggcg
    15601 aggatgccag ccggcaacaa ggcagcaatc acattgccga tcccgataaa catgctttgc
    15661 atggtgtatc cctggttgat ctggtgctcc ggcaacatat ccccgacaaa ggccctgaac
    15721 ggctccatgc tgatgttgat gctcgcgtcc aggatccaga gcaggccggc cgccatccac
    15781 aaggctgaag agtgtggcat ggccagcagg ccaagggtag ccaatatcgc ccccacggtg
    15841 aaatagggct ggcgcctccc ccattttccc caggtccggt cactcatata cccgatgatg
    15901 ggctgcacca aaagccccgt catcggcgcc gccagccaca agagcggtat ttcatccggt
    15961 tttgccccca ggtattcata gatggcgctc atatttccca tctgcattcc ccagcctacc
    16021 tggatgccaa gaaatccaaa actcatgttc cagatctgcc agaatgaaag ggtgggtttt
    16081 tgattcatat tcatcaggta tgattcttta ccgaaagtaa tgaaaagctg tgagctttga
    16141 cctcgagaag tcccgggaga attgtgagca cccctcattg attcctgatt cctgactacc
    16201 gattactgat tacttgttaa ctttcaaact tgctaacttg ctaactacaa catgaatgct
    16261 gtcataggcc ttgattttgg caccgattcc gtgcgggccc tgatcgtcga ttccgaaacg
    16321 gggagggaac tcggaagcca ggtggaatat tatccccggt ggcaaaaagg gctttattgc
    16381 aacgcttcaa aaaaccaatg gaggcagcat cccctggact atatggagtc tatggaagcg
    16441 gctgtgaaag cggtgatcaa acaggcaggg ccggaaatcg cccgttccat agtaggcgtc
    16501 ggcgtggaca ccaccggttc caccccggta gccgtggacc ctgaagggac tccgctttct
    16561 ctcttaccgg gtttcgagga caatcccaat gccatgttta tcctgtggaa ggaccatacc
    16621 gcagtccagg aagccgcggc catcaacagg ttgtgcagtg attgggagac tgattatacc
    16681 aaattcgaag gaggcattta ttcttcggaa tggttttggg ccaagatcct gcgcatctgc
    16741 cgcgaagatg aagccgtgag gaaggcttca tggtcctggg tggagcattg cgactggatc
    16801 ccccatttgc tcacaggcgg aagggacgcc accctgatta aaaggagccg ttgtgccgcg
    16861 ggccataaag ccctctggca tccttcctgg ggcggtcttc ctcctcaatc ctttctgact
    16921 gcccttgatc cggtattggg cggtttgcgc gaccgcttat ttaccgagac ccatacctcc
    16981 gacgaggcgg tgggtcatct ctccggagaa tgggctaccc gcctgggtct ccgtaccgat
    17041 gtagtcgtgg cggtgggcgc cttcgatgcc cacatggggg ccgtcggggg gcagatcgaa
    17101 ccttatcacc tcagcaaggt gatgggtact tccacctgtg atattctcgt ggccccgaac
    17161 gaagaaaacg aaatgctggt caaaggcata tgcggccagg tcgatggcag tgtgatcccc
    17221 ggcatgctgg ggctggaggc aggccaatcc gcttttggcg atatctatgc ctggttccgg
    17281 gcggttttgg cataccccat ccatgaaatc atcggcagca gcggtttgct ggatgaaacc
    17341 acccgtggaa aacttatccg ggaatctttt gatagaatca ttcccgaatt gaccaaggcc
    17401 gcccaaaaag tacccatcgg ctcagggggc atcttatccc tggattggat gaacggccgc
    17461 cggaccccgg acgccaacca atatctcaag ggtgtgatca gtggcctgaa cctgggcagt
    17521 gacgccccgt ccattttccg ctcgctggtg gagtccactt gttacggagc ccggctgatc
    17581 gtggaccggt tccgggcgga gggcattccc attaaaggat taataggcct gggcggagtc
    17641 gctaaaaaat cacccttcat catgcaggtc atggccgatg tcatgaacat gcccataaag
    17701 attgccgctt ccgaacagac ctgtgccctc ggggcggcca tgtttgcagc agtggccgca
    17761 ggggcccagc cggatctggc ttccgccatg cggaacatgg gatcgggatt tgatgcggaa
    17821 tacttgccag tacccgaaca tgccaggctt tatgaaaaat actaccggga atattgcagc
    17881 ctggccgggc atatggaatc ccatgtcatg aatgaaatcc atcaaaaccc atgaacaaat
    17941 acgatgccct gaaggaagaa gcctttgcgg ccaataaaca attgcccgcc ctcggcctgg
    18001 tcctttttac ctttggcaat gccagcgcgg tggaccggag ggaaggggtt tttgccatca
    18061 agcccagcgg gatcgcctat gaaaaactaa ccccggacca aatggtcatc gtggattttg
    18121 aagccagggt ggtggatgga acgctgcggc cttcttcaga taccaaaacg cacgccgtat
    18181 tgtataaaga atgggaaggg atcgggggta tagcccatac ccactctact tatgccaccg
    18241 cctgggctca ggccgttcag gatattccca tcctcggcac cacccacgcc gatcacctga
    18301 ccacggacat cccctgcgct cccccaatga aggatgaata catccacgac gattatgaat
    18361 acatgaccgg gtaccagatc gtggaagaac ttcgggagcg ccaatggtct tataaagaag
    18421 tggaaatgat cctggtcggc agccacgctc cttttacctg gggaactacc gtacagaagg
    18481 cggtttacaa cagcgccgtg ctggaatcca tcgcccggat ggcctggctg accttgcaga
    18541 tcaggcccgg agtgccttcc atgaaagata ccctcattca gaaacattac gagaggaagc
    18601 atggggaggg gaggtattat gggaatgagg aattcaagtt ttgaagttcc gaatccatct
    18661 tggcttgctt acatctatct ttgactgagg cgttccagaa ttttgccaaa agagtacttt
    18721 caatttataa tttattcata aaaaaccatt tgggttgttt aaaaaattat acaatttgta
    18781 caattattat tatctttgtt gataaatttt aactatggga tgaaggtaaa aatcattaga
    18841 cttagtgacc accaggaagt ggttgcaaga attgattttc cgaaagggat tcctttgcct
    18901 tcaataacgg atggatggaa atttaatttt aaaagccact ctaaaaaaag aggtttaata
    18961 acatacatcc tggtgtgtga agatactcca aataaaatag agggctgtat cattttccaa
    19021 ttaaaagatg caattgaacc atacatggcc tatatcgaaa tagctcctca taataaaggt
    19081 gaaaagaaac attttgatag ggttgcagga tgtttaatcg catacgcttg ccggcttagt
    19141 tttatacatg gaaaaggacc ttataaaggc tggttgacat ttgatgtatt ggaagaagat
    19201 aaacaagatg aaataaaact catggctgtt tattgtaaaa agtacggagc ccaaaagttt
    19261 ggtgatacta ccatggtgat atcccctgag gcgggtgaaa atcttataaa caaatattta
    19321 aattaaaaac ttatgaaaag gaacgaagtt agaataactg acctttggaa taaagaaaaa
    19381 agcaagtctc ttagtgaatt tattattgat caatccaata aacagacgaa agaacggaaa
    19441 ctaagaaatg aacttttgtc aatacaattt aaaattgaag attacattga gtcagaaaat
    19501 gtaatccaaa aattaaatat tttggatttt gtcaaaatgt atcttagcgc attgaatatt
    19561 acccaaaaga gattggctga tttatttgag atgaaagaca gtaatcttta taagtattta
    19621 gttggcgaaa gaaaattaaa tacaacaata gttttaaagc tgagttcatt tactgattta
    19681 aacccagagt attggctaag aatagaggtt aagaatgaat taatagaaat aaacaaagag
    19741 aaaatcaaaa taaaattttt caggaaatat aactatcaaa atttgttgac cgttcaagaa
    19801 gattaaggtt gctctcctgc aatatattta cacaaccaat cttgaagaaa agaatgctca
    19861 aaataagaga aattggaata ttaggtcact ggatttcaac tattgtcacg acacaatact
    19921 ttaaaaaaag atttaccaat ccctgacatc tgactagcca ggaaatcgca agttggccat
    19981 aatttcagag gccagaccac ccatccagaa atccactatt tttagtccta tgaattccaa
    20041 gatactactg atcgcccagt tgtttttgat atcaaccggg ttaccggcac agacctttgg
    20101 catccacgaa ctcaatcaaa aactgggcaa gggcatcaat atgggcaata tgttcgaagc
    20161 cccggcggaa ggggaatggg gcaatccgtt ccgggatgat tattttcaaa agatagccgg
    20221 cctgggcttc aaccacgtac gcattccgat ccgctgggat gtcgctgcaa gggccctgca
    20281 aacggcgccc tttaccatcc agccctcctt tctggaaagg atcaaatatg tggttgacct
    20341 ggctttgtcc cagggccttt atgtgatcat caatatgcat caccacgagg ccctcttcca
    20401 ggatccggaa ggagtcaaac ccaggttcct gagccaatgg ggccagatct ccgattattt
    20461 caaaaattat gatcagcgat tgctttttga agtgttgaat gaacccaata ccaacctgac
    20521 cgggcaaaaa tggaaccagt tctttaaaga cgcgctcggg gagatccgga aaaccaaccc
    20581 ggaccgggcc gtcctcatgg ggatcgctcc ctggggcggc ctgggcgggg tgcctgacct
    20641 ggtggtacct gaagatgaga atatcatcat caccgtgcac tattatgatc cgttcacctt
    20701 tacgcaccag ggagccgaat gggtggacaa ttccacgccc tggctgggca ccaaatggga
    20761 aaacaccacc ctggaacgga atgaaatcac cggctccttc gcttacctga tcaattttgc
    20821 caaatcaata aataaaccca ttcacgtcgg ggaattcgga gcctactcaa aagcggacct
    20881 gaacagccgg gtcctctgga ccaattttct tgcccgttgg tttgaatccc agggattcag
    20941 ctgggcttat tgggagtgga gcgccggctt cggaattttt gacccgttca cgaatcaata
    21001 cctgacccgg ctggccgacg ccctgttaaa ggatcccatg ctgcccgcca aaccacaaac
    21061 cacggtaaag gtttttgaaa gcggatttgg cccgggagac ggctggaacc tttatgtgca
    21121 gggaggtgcg gcggcaagct tgtcgagaac gggtggacag ggcatcgtgg atgtcactca
    21181 aaccagcggc accggatggc atgtccagtt ggtacagaac aatatccagc tgaaaaagga
    21241 cagccgttac ctggtcaccg tcaaggcttc ttccaatacg ccggtttcca tcacaaacta
    21301 tctcggcatg aatttttctc cctggggaag ttattcgggc tacaaatccc tgtccctgaa
    21361 cacagcggaa acggaacaga cctacagttt tatcatgacc tcccctaccg atccggcggc
    21421 ccgcctggtt tttgatctgg gaaataaacc atcccgcata accatctccc aggtaaaagt
    21481 ggaagaagtg ctggatgtcc cggtatccta taaacagacg atatcccacc ctataaaagt
    21541 atttcccaat ccctctgccg cgcggatacg gatcgaaggg atagaccaac ccggcaggct
    21601 ccagatcctc tcagcggatg gcaagttgtg cataaatttt ggaccatgga atcccatgaa
    21661 cacggtcgat gtcgccgaat tgatcccagg gatatatttt ctcaggattg tgaacagccg
    21721 aggtattttt aacactcctt ttattaaata ttaaaccatt cagattgaat ttgtgagaga
    21781 tcagtttcga gatcccagat gcgagatgcg agatacgaga tgcgagatgc gagatatgag
    21841 atacgagata tgagatacga gataagaggc tctttaccca ctaacttgct aacttactaa
    21901 cttactaact cgccaactta ccaacttgtt aacttaccaa cttacatccc atgatcaagt
    21961 cactcaccga atttgaggtc tggctggtga ccggcagcca gcatttgtac gggccggaaa
    22021 ccctggccca ggtcggccac catgtaaaac agatcgccca tttcctggat cagcagggac
    22081 ccatcccggt acaagttatt tcgaagccgg tcgtaaaatc ccccgaagaa atttaccagg
    22141 tggtcaagga agccaatgcc agtgattttt gcattggcct gatctgctgg atgcatacct
    22201 tctcaccggc taaaatgtgg atccgcggat taaaagccct tcaaaaaccc atggtccacc
    22261 tgcacaccca gttcaatgaa caaattccct ggtccgcgat cgatatggat tttatgaacc
    22321 tccatcagtc agcccacgga gaccgtgaat ttggcttcat tggctccgcc ttgaaattgg
    22381 aacgcaaggt gatcgttgga cactggaaag aggaagaagt gatccggaaa ctgggacgat
    22441 ggacccgggt ggctgcagcc tgggccgaca gtcaaaccat gaaaatcgcg cgtttcggag
    22501 acaatatgcg ccaggtggcg gtcacggaag gtaacaaggt atctgcccag gtccgttttg
    22561 gatatgaggt cagcggttat ggtttgggtg acctggtcca atacattgat tcggtcacgg
    22621 aaagcgaagt gatccacacc attcaggaat atgaagacca ttataccctg atgcaaagcc
    22681 tggggcacgg gggggaaaaa agagaatccc tttttgaggc ggcccgtatt gaagctggat
    22741 tgagacattt tttgcacgac ggaggattta ccgcctttac cgataccttc gaggatttac
    22801 acggattgag tcaattgccc ggtatcgcag tccagcgcct gatgcacgaa ggttatggtt
    22861 ttgccgcgga aggagactgg aaaacggcag ccctggtgcg aaccatgaag gtgatggccc
    22921 tgggcctgga aggaggcaac agtttcatgg aagattatac ctaccatttt gacctgcaag
    22981 ggtcccgggt gctcggcgcc catatgcttg aaatctgtcc ttccatcacc cggcaaaaac
    23041 cacggtgtga aatccatccc ctgagcattg gcggcaagga agatccggtg cgtctcgtct
    23101 ttgacggggc ccctggaaag gcccttaatg cctccatgat cgatctcggg caccggtttc
    23161 gcctggtcgt caaccaggtt gaagcagtgg acgtcgggca tgaaatgccc ctgctcccgg
    23221 tagcgagggt gctttgggat tccaaacccg ggctcgcagc ttcagcggaa gcctggatcc
    23281 tggccggagg cgcccaccac acctgcttca gccaaaacat caccatcgaa gacctcgaag
    23341 actatgcctc catggcggga attgaaatcg tggtgattga cgaaaatctc ccgcccctcc
    23401 gggattataa acagatgctg aggaataatg agatttatta tgggttgagg tgaatggatc
    23461 gcttaaaggc ttcacaagct ctgcgccttt agcggttaat tcaaatcgtt ttagcctacg
    23521 ggatatggca gagggaccta ttgtacagac cttcatacca caggtaaaag cccccggata
    23581 atacacctgt ccaacttagg aacttactaa cttgccaact ggctaactga ctaactgact
    23641 aactttgccc tccgtggacc attttgtatt catcatcaac aaaagttccg gaaggcagaa
    23701 aagcggggag ctggagtcgc ggatcatggc tgctgtcaaa gatcgcccca tgagcgcgga
    23761 atgccacatc acccagaatg cagagcatac cgctgaattg gtccagacgg ccttagcccg
    23821 ggggcgggaa aattttgtgg cggtcggagg tgatggtaca gtcaataccc tggcccgcca
    23881 cctggtccac cggaaagcaa tcctggggat cattccccgg ggctcgggca atggcctggc
    23941 ccgtcacctg ggcatgcctg caaacataaa cgatgccatt gaacggttgc tcccgcgcag
    24001 gacccagtcg gtcgatgcca tcctgatcaa tgatcactgg tccttcaatg tcgcgggaat
    24061 tggtttcgac ggatatatct ccagcctttt cggccagaat ggaaagcggg gcgtggccaa
    24121 ttatataaga ctgatcaacc aggaatacaa atcgtatcaa ccgattgaga tggaaatcca
    24181 caccgatggc cggatcctcc atgaacaggt cttccaactc gccatcgcca atgccagcca
    24241 atatggcaac agggccatca tcgctccttt cgcggccctc gacgaccagt tgcttgatat
    24301 ttccatgatc tccaaagttc cctatacctt cctgcccggt tttttccttc gtgtgattgc
    24361 cggcaagatc cacaagagcc gttactggaa ttcttttaaa accaaccgca tgtacgtaaa
    24421 gacttcccgg ccggtgcatt ttcataccga cggggacggt cgcggcatat cggaacattt
    24481 taatgtggag ctggtgccct cctgccttaa actgatctat tgatcgcaaa gtggttgtca
    24541 tttagcgcca gccccaagcc cagcaagtgg caatcactga gcgcaagcaa gcggttgtca
    24601 cttagcgtca gcctcgagcg cagcaagtgg caatcactga gcgcaagcaa gtggttgtca
    24661 cttagcgtca gcctcgagcg cagcaagtgg caatcactga gcgcannnnn nnnnnnnnnn
    24721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24781 nnnnnnnnnn nnnnnnnnnn nnnnncagca agtggcaatc actgagcgca agcaagtggt
    24841 tgtcacttag cgtcagcctc gagcgtagca agtggcaatc acttaaggca gccgcaagtt
    24901 tcgtggcaaa aatatttttc taccaggccg gagctaagct cctcccaaac attaagtatt
    24961 gaacgcagtt ttcgtttgac aatcaaataa gcattgctgt gaaggcttca taaaaatttc
    25021 tacaagcatc cggatgactg tccaggaaaa gcgctggttc gatcagttcc agttccatga
    25081 gcacaaaacg attacaggca tccaggtatc catcgatgcg ggcataaagc aaggtctcag
    25141 ggatcagttc taatattttt tcaacttgca ggaaaagttc atattccgga aagtaggggt
    25201 gatattgacc cccgtattgg gtttgcaccc ggaaatcacc cggcttcgct tttttgcaga
    25261 ttgcgtgact gaatttccgg ttaaaataaa tcagggacaa ttcaccatgg cttacgatct
    25321 cttccgaaaa tctctgcaaa atcacatcct gttcattcaa taaagcgtcg aatgccgtct
    25381 gatgcctgtt cgccagatcc ggggtggtca cccaggtctt gtatgctcct ccggatatgg
    25441 tgggcttgat caccgctttc tcaaaaccca gctgttccaa ccatcccttc agatcagcgg
    25501 aatgtccctg aggtcggatg attccctccg gaataagaat gcccttttcc ttaaaccacc
    25561 cgaggtaggt tttattttta ttccagcgga ctaccgagag cggattaaaa atgggcttct
    25621 tgaccgtttc cattttttcc agccagcggt caaattcatg aattctttta aaataatccc
    25681 agaccgaacg aataacgatg gcatcaaacg gatcccattt tatatccggg tcgtcccaaa
    25741 cggctgcaat gacccggtga ccctgctttg caaaccagtt gaccaggaga cggtcatccg
    25801 gggtaagatc gtcatgttcc cggtccgtaa cgaaagcaat gttcatagca agtgggatta
    25861 tatcagacgg ttggctctcg acccgggcat gagttgccgc cgactaagat caaaaggatg
    25921 aatctgaacc acgccggggg tttttcctgg agaaaaacta cccaggtctt tttcaaaccc
    25981 aagcgccagg gccccattca gcgtggccca gcggatcagc cggtcaaaag tcaggtaaga
    26041 ctgataatgc tgaatggttt tcaattcttc aaggatggag agttgccagt tggaagcaag
    26101 gctgtccgta cccacgcaca ttttggctcc gtgctcaata aaatgcaggt accggggcag
    26161 ttgattctcg atataaaggt tggcattcgg acaggtgacc caatacactt cctcattcca
    26221 ttcctccaca gaccggatat cccgcggagt actgatcgta ttgtgaacta gaagggtcct
    26281 gcgcccggcg ctcatatttt ggatcacctg gagggaggaa ggcgcccgcg tggcttcgaa
    26341 agcttccaga gataccccca gggtctggta gaagtcgatg agggcccctg atttttccag
    26401 gaaaaaatgg ttttccgcat gggtttcctg gtggtgaatg ctgatgctct ccccttcatg
    26461 cggatgagtt ctaagcaatt caaacaggct ggaggagacg ctgtaggggg catggggcac
    26521 cagggatttt ttatgtcccg gtggacaatg aagttttgac cagacttcat atgccctggc
    26581 catttcggct tccgcctttt catcctggag aaggtcgaaa gcctctacaa aagaatgata
    26641 cctcaaaagc ccccgtgatt tttgataaaa cgtgtcctcc acattgctga tgtctcccac
    26701 cgcaacgatc ccgcctgcga tcatttcctg ttcgcccgcc gcgatcgcct cgaggatgcc
    26761 ctgccggtcc acttctttgc gccgccggac gatatttttt ataaactcca ccaggcctgt
    26821 cccggtagga atcatgttct tcatatgaga cagttccagg tggcaatgcg cattgacaaa
    26881 gcccggaacc aggataccct ctataaattg gatttgcgtt ttcctgtcct ccacccgctc
    26941 caggggcagg atatttaata tttttccctt gtcatcggtc tccagtacat ggccctccag
    27001 gggatccggg tgttcgctcg tgtaaatcag ggaagcatga aaaagcggca tgactaaagt
    27061 attgcagatg cgaaaataat ggataagcta ccaatccaat caacgagatg ccagaagcga
    27121 gcagcgagct acgagcagcg agcagcgaga agcgagaagc gagacagacc tgctaacttg
    27181 ctaacttgct aacggttgat ggcaatataa aaatgagtca gcaatttttt tataatatgt
    27241 tatcagt
//
LOCUS       DAOQXT010000064        26887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2108_length_26887_cov_6.840042, whole genome shotgun sequence.
ACCESSION   DAOQXT010000064 DAOQXT010000000
VERSION     DAOQXT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 26887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26887
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2108_length_26887_cov_6.840042"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1270)
                     /locus_tag="PKM27_11845"
     CDS             complement(35..1270)
                     /locus_tag="PKM27_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNR08001.1"
                     /translation="MKNLFKKISIALAAVGPGLFLIGYNIGTGSVTTMAKSGADHGMS
                     QFWALVLSCVFTYILMVAYGRLTLVTGRTPLYNFKHEFKFGWILALYVLVALVVGELF
                     ALIGVTGIVADLLQEGIKIVSGGTVVHTGWIILVIVVLMYLLLWFGRYKQFEKVLTGF
                     VVLMGLCFVLVFILLKPDFATILKGMVPHIPDQPGALGLIAAMAGTTCSAAVFIVRGT
                     VVAEKGWTAKDLPAEKRDAFVSAFMMLFLSGIIMAVSAGTLHVMGLRLNNTVEMIHLF
                     EPLGGKAAALILIMGVTGAGLSTIFPIVLIAPWLVSDYLGKPRDLKSPLYRWLILLGL
                     VFCFGTLVLKQGAPALMIFSQAFQACILPAVAIPALILLNRQNTMKGITSGWKMNLGV
                     IAVILFSFLTTWFAIVDLI"
     gene            complement(1310..2353)
                     /locus_tag="PKM27_11850"
     CDS             complement(1310..2353)
                     /locus_tag="PKM27_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR08002.1"
                     /translation="MAPGFFDNQVNGFAGVSFSFGNSDLRPEDILKATRELWKKGVTT
                     YLPTLTTHSHEVLMKNFKVLGQNYSGDSLLGSIPGFHLEGPYINPEDGYRGAHPKAYV
                     RLPDWEEFMKLYHASGKKILQVTLAPELEGAMEFIKKCKELGITVAIGHHNANRSQVD
                     EAVGLGARIATHLGNGCANMINRHLNPLWPQLANEDLLISIICDGFHLNEEEIRVFYK
                     VKGPERTIITSDVTSYAALEPGEYKTQTGETIELTREGMLRYPAQNVLYGSASPITKG
                     VGHILKVTGCSLAEAVQMASTIPARLYGLTDRGRLEKGLRGDLVLFTLEDFKLTIQKT
                     YVQGKKVFDLNHP"
     gene            complement(2560..3540)
                     /locus_tag="PKM27_11855"
     CDS             complement(2560..3540)
                     /locus_tag="PKM27_11855"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08003.1"
                     /translation="MKSRRKFLNTLGTGALLASSGIPDLKAFSGFRTSKPIRIGIIGA
                     ENSHTAAFGKLFNIDKKFPGVEVTMVWGETDEYAQKSMKAGNIPIQVKETAEMLGKID
                     ALIVDHRHAKYHLEPARPFVEAGIPTFIDKPFCYRASEGLAFLQMARKKGTPVTSYSS
                     VAHSDATLDLRTQVKAMPDISQVIITGPVDPDSPYGGIFFYGVHMVEPLMVIFGEDIQ
                     AVKVSRGGKAGNALLKFASGLTATLLFKNKAYGWETYVETSKAMVELKPKVKESDPPR
                     ADADMVQMFATGKEPRSQQSILNGISVLEALEKSVLSEKWTDVEYLNIEG"
     gene            complement(3596..4954)
                     /locus_tag="PKM27_11860"
     CDS             complement(3596..4954)
                     /locus_tag="PKM27_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08004.1"
                     /translation="MTMKTKSSRSRRRFLRNTVAGAGLLAGGVLPGFSASSYRRILGA
                     NEKIRASVMGVNSRGFALSQNFAYQEDCDVVHVCDVDHRASAKCIKGLEGIQDLKPKA
                     YGDFRKSLESSEVDVLVVATPDHWHAPAALLAMQAGKHVYLEKPCSHNPQEGEILVKA
                     ALKYKKSVQMGNQRRSWPNVMEGIAELKNGIIGRPYFGKGWYTNNRESIGTGKPAPIP
                     DWLDWELWQGPAPRKPYQDNVVHYNWHWFWHWGTGEALNNGTHMIDLLRWGLGVDFPV
                     KVSSNGGKYHFKDDWETPDTQVINLDFKEGSSMSWEGRSCNGRHIEGSSVGVIFYGET
                     GALMIPSGNEYRVYDLKNKLVKEKKDSRPVDPRDLANPAGHLDALHIRNFFNHISKGE
                     KLKSDIDSGHKSTLLVQLGNIAQRVGHSLEINPENGHILGDAGAQKFWSREYEKGWEM
                     KL"
     gene            complement(5076..5576)
                     /locus_tag="PKM27_11865"
     CDS             complement(5076..5576)
                     /locus_tag="PKM27_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08005.1"
                     /translation="MFSIWMLIKALHILGGTFWLGGAAMIAAFLLPAAKKTGPSAGVF
                     MGHLMRDLKLPLAMNLASWTTLLMGTLLYWRITGGFKQWSLHWPGGIGLLIGVISGVI
                     AFLWGTFIQAPNAQKLGALLVQMQQSPSPELGAKVQAMQKKIYLGAWVGVLFLVLSLI
                     GMTLLH"
     gene            complement(5709..6503)
                     /locus_tag="PKM27_11870"
     CDS             complement(5709..6503)
                     /locus_tag="PKM27_11870"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNR08006.1"
                     /translation="MDLFYRCLAKTSVSQTVENVHRLRVAIKGIRSLLVLMDKACAGA
                     LQKRDHLKIYAPLFRKAGQVRDIQISLGLLKSKKTAYLVPYKKYLGRILKVKTRHLHA
                     ETLVIDLKQLDKLNHELVNKIQNIHPGSIPQSAASLIQRLLGRVYSLSQKPRDVKTLH
                     DIRIILKTVQESLEILLRIKPSQDWKAVDRKLKPMYDALGKWHDEALLIKSIKAYQHS
                     SGSVNGHLTRLASRLEKENKTREDKIYALLRKLLPPFRQKGSRKAR"
     gene            complement(6600..8894)
                     /locus_tag="PKM27_11875"
     CDS             complement(6600..8894)
                     /locus_tag="PKM27_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08007.1"
                     /translation="MLKPLILWSCLIFKAWTLFGQSPERVYYSSFRPEGWDIYLSGDG
                     GKSFKPIAEDPALDYDPVISPDGKWLVFTSEREGMPRLYIQSIDQAERPRLLVNSASM
                     QDQLVFSPDGKWCAFVSTHQGNAEIYKLPFVPDSVMDIRQAINLTNHPGGDFRPAWSP
                     DGSTLVFSSDRDHAVKPHAEFPFAMYRTGNLYSMDTSGSALERLTRTETWDGSPKWSL
                     DGKQIYFYSLRSGMPQIYQMNKDGSSQKRISPVDLKAISPCPLNDSLLVFTHWTQEDR
                     GAFKFMVLNLNSLNTEIYYRDSLAMYGMVAGPKDLRAFHGGKIPLEEKTNAGGFAGHL
                     LVRGPMEQYALPEKTVRLYGVRRAFAAPPDPHSPSLVFAAEQSKSIQEVLLTKYSAVL
                     LLFPAGLLLLIFFGLYRAIRDRLSVKPWKNVLAMSALLILSIAVLTISGYEIFASSNS
                     LSRSRTTFLLLAIPFLILCFYFFRKGGKASVLNSPFQIVYRTLGFGTGLLCMMLVYLI
                     VFYSNLFNLNIDFLKVNHETGAVEWLFTFKPGHSKVSQILDIKYKPDGSSLLFTAGLF
                     RNRPSAPGEIWEYRFMDKELKKISLFGSNQGFADFSADNTSMLFRGGHTGNFDIYLKT
                     KDQIENLTHSPWRENFPALSPDGNKVVYCSDINGKRVDSTITTMNIHFLEKQPDRSWG
                     ASKQITHFEGQEAHPHFSPDGLYIIYTTEEFGINDEQPLSQEFFFSPQLYGEIVAKRL
                     DDGQTFRLTHNKWEDGAPLWVKGY"
     gene            complement(8999..11284)
                     /locus_tag="PKM27_11880"
     CDS             complement(8999..11284)
                     /locus_tag="PKM27_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNR08008.1"
                     /translation="MGHLPKLIEDLALILIVGAMVTILFRRIRQPLVLGYILAGFLVG
                     PHLSIFPTVADMENVETLAEIGVIFLLFSLGLEFSFKKLMRIGGTASITAGIEIVFIT
                     LAGYITGKLMGWTTMDCLFLGGMLASSSTTIIIRAFDELGVKTKQFARVVFGVLVVED
                     IVVILLMVILSTVAVTRTIEGSQLFLTITRLLFFLILWFIAGIFILPTFLKRVKTLLD
                     EETLLILAIGLCLGMVVLATQVGFSAELGAFIMGSILAETTKAEKVEHLIKPVKDLFG
                     AVFFVSVGMLIDPLAIKEYGWAVILVTLLTIFGKFFSTAAGALISGQTLKQSVQVGMS
                     MAQIGEFAFIVATLGLSLGVISDFLFPVAVGASAITTFTTPYLIRYADKAYDLIARIL
                     PKRWVNALDRYAYSTQVSQVEGAWQVAAKSYVNIILINLIVLAAILLLSVKYLLPLLI
                     QWSDREVVGRGIALGLTLTVSLPFLWGLMAKRPGNMTYRELWLNRSASFGALRIIDIL
                     RIIGGVLLIGFIIDQFFSTLVSLIAVPLILGFLLPWAHKIQDLYHRLEDRFLDNLNAR
                     ESAAKANLTAEKRLHQKFTSSLKLSAWDAHMIDLKVDPHSYSVGKSLEELGWREHYGV
                     NVAYIRRGDKLIYAPDRNQKIYPHDQLGIIATDEQIQQFKPAFDTCESIEPAEPHVDD
                     IVLQKIQVDESNLLAGQTIRNSGIREKTKGLVIGIERGSQRMINPRSDLLFEEGDIVW
                     LVGERHKIHEQFSIMAPVHEH"
     gene            complement(11391..11912)
                     /locus_tag="PKM27_11885"
     CDS             complement(11391..11912)
                     /locus_tag="PKM27_11885"
                     /inference="COORDINATES: protein motif:HMM:NF039358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6141 family protein"
                     /protein_id="HNR08009.1"
                     /translation="MENQVFFSEMQRFKKWWVWLILLALIALYTYGVISQVLVGRPFG
                     QKPMTDAVLLLTLGLILFFAVLIYNIRLDTEITKEGVQVRFFPFHLRPRRYPWNSIRR
                     WEVRKYNAIIEYGGWGFRLGIFGKGKALSISGDQGLQLEFNDDKKLLIGTRKPKEMEA
                     AILRARQPKEPEA"
     gene            complement(12054..12716)
                     /locus_tag="PKM27_11890"
     CDS             complement(12054..12716)
                     /locus_tag="PKM27_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNR08010.1"
                     /translation="MQRIFIFAAVLIGLQAGLIAQQAPFWSEILAFSRQDEQQWPGTG
                     MIVFTGSSSIRMWKTLEQDFSDHRVLNRGFGGSTLPDVIRYAGEVILKYQPGQVVIYC
                     GENDLAADSSISGKTVFKRFKSLTGIIRDSLPDTKILFIALKPSPSRIHLMEKIVDAN
                     RRIRRYIKSRKYLAYADVFTPMLGPDGLPPERLFIKDRLHLSREGYEIWKETVKPFLI
                     RD"
     gene            complement(12768..13733)
                     /locus_tag="PKM27_11895"
     CDS             complement(12768..13733)
                     /locus_tag="PKM27_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HNR08011.1"
                     /translation="MININDLMVTGKKATRDGFGTGLLEAARQNKEVVGLTADLMGSL
                     KMDEFKKEFPERFFQMGIAEANMMGVAAGMAITGKIPYTGTFANFSTGRVYDQIRQSI
                     AYSHKNVKICASHAGLTLGEDGATHQILEDISMMRTLPGMTVIVPCDAYQTHQATVAI
                     SKHYGPVYLRFGRPAWPIFTEALPFEIGKAQVLNEGSDVTLVATGHLVWNGVEAVKLL
                     ALEGIHCDLINMHTLKPLDQSTLLESAGKTKCVVTAEEHQINGGLGDAVAAVLARNLP
                     VPQEFVAVQDSFGESGKPDELLIKYGLGVQDIVKAVKRVLARKKA"
     gene            complement(13761..14609)
                     /locus_tag="PKM27_11900"
     CDS             complement(13761..14609)
                     /locus_tag="PKM27_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNR08012.1"
                     /translation="MKKDLDSLRALASQVRRDIVRMVYQCQSGHPGGSLGCTEYMVAL
                     YGAHLRYNLPFNMEGKNEDLFFLSNGHISALWYSVLSRHGFFPVSELATFRKINTRLQ
                     GHPATHEGLPGVRVASGSLGQGLSVGVGAALAKKLDSDPGLVFVLTGDGELDEGQNWE
                     AIMSAAHHGVDNLIVTVDWNGQQIDGPTEKVMNLGDLPAKWRAFGWDVLLLENGNDME
                     EVVRSLDEAKSRLHQHKPVVILMKTVMGYGVDFMMGSHKWHGVAPNEEQCKNALAQLP
                     ETLGDY"
     gene            complement(14740..15036)
                     /locus_tag="PKM27_11905"
     CDS             complement(14740..15036)
                     /locus_tag="PKM27_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNR08013.1"
                     /translation="MFNQNSNKMNKSDLIDAIAKGASITKVQAEAALGSTLDSIRKAL
                     KKGDRVQLVGFGTFSVSKRAARTGRNPQTGKTIKIAARKVAKFKAGKELSTAVK"
     gene            complement(15169..16374)
                     /locus_tag="PKM27_11910"
     CDS             complement(15169..16374)
                     /locus_tag="PKM27_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08014.1"
                     /translation="MKIRFAQYWQNKSATYRAALLVIVGSLFIAAIANLVANEKPLFC
                     ICEGRWHFPAWRDYAEYTGLLRKTTAFEYTDCTHRLPAPVPYSPSTIDPDGSGYLSPS
                     AGWALHGKKHWLGTDALGRDVLAGMIHGTRYAWLIGLLSTLLAGIPGIFAGMAIGYWK
                     DHKLKLTLFQGIGLVLSFLLWIYEFLMIGSLSSGMVRMVLLVAWTALFIWVCSKWLKP
                     GRRSGLKTFSLPVDSLFMRFIDYFESFPRLFLMMALLVFLARPSGALLIVLIAFIRWP
                     LFAQVARAETLRESALNYVKASENLGLPWYRILSRHIWPNIRSTLLVTALFSVSTAVL
                     LEASLSFIGLGLKLESVTWGSILNEGRQYLPAWWLSVFPGLAMFILLLSLHWILDEKS
                     RNGAHREGW"
     gene            complement(16371..17729)
                     /locus_tag="PKM27_11915"
     CDS             complement(16371..17729)
                     /locus_tag="PKM27_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08015.1"
                     /translation="MKRLILNRLLISVPLLLAISFTAFILRDFMETDAFDPLQESQNL
                     PVSLRRSEIIQRARAESRDLPLFYFSIRPWSYPDTLFRFVIKSEKKRIKRFLHQGVPW
                     PSVHVYVTLEREMVEKAAHRLDKLLVEELSRLDDLNAMSAKLDAMTDSTRAGVLWGKL
                     KSSLHEMQKSRKWFPLFVPVPGWHGSHNRFHIWLNQIMRGDWGVSRSDYRPAWVKIRE
                     AVSWTLSINLVSLLVVYLLSLFLGEWLFRRKEQHKGKIIESVLFFLYSIPRFFLALLL
                     ILAFASDTLSPALHIFPSPGFFDSNPEASLLEQWSRYGAQLVLPVICLVLPSLAYLTR
                     IYSIRLRDEKNKPYAFMAWSKGDAEDFITRRHLRRNALLPLIALLGMEIPALFGGSVV
                     IEVLFNIPGMGRLMLQSILIQDWLIVFNILLLIGTFTILGKLLADLLYGFTDPRIRWR
                     AS"
     gene            complement(17749..20133)
                     /locus_tag="PKM27_11920"
     CDS             complement(17749..20133)
                     /locus_tag="PKM27_11920"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNR08016.1"
                     /translation="MPNPYSFFIFLLSIPALVRGQEHILWYSRPAVFFEEALPVGNGR
                     MGAMIYGGIREERLSLNDITLWTGEPVKADQNPESFRLLPAVREALFREDYAAADTLV
                     RKLQGRFSESYAPLGNLFIDFHHPHEASEYRRQLDLQNAEVNIQYVSNKTLYTRTVFA
                     SHPDRVIVIRLDTRGEGRLHFTVHLNSLLQHQVHTGAHRMTMAGRAPVHAEPNYRRVN
                     NAIVYADQRGTRFAASLEIRHGGGPKHATDSTITIDQASWAEIRLTIATSFNGFDKDP
                     FREGLDEIKGAMDPLEKISKYSYSDLVQRHRADYSKFYNRVVLDLDPAPSEKTEIGLR
                     LKQNRVERPDPGLFELYFNFGRYLLISGSRTPEAPLNLQGIWNEQVRPPWSSNYTVNI
                     NTEMNYWPVEVCNLSELHQPLLSFIGNLAQTGAVTAKTFYNCRGWTCHHNTDIWGMTN
                     PVGDFGSGDPNWANWQSGGAWLVSHLWEHFLFTRDSSFLRTRAYPLMKGASLFCLEYL
                     VEDHQGFLVTSPSTSPENIYVTDKNYKGATLYGSTSDLSMVRELFHAMIQAGEILDTD
                     HGFLDSVRSALNRLYPYQVSRDGHLQEWYHDWADQDPRHRHLSHLYGVYPGSTITPDQ
                     KALFQAARQSLLKRTNNGTGWSISWKIAMWARLLDAEQAYDAFLKLLTYYPADQSEIR
                     MAGGGTYPNLFDAHPPFQIDGNFGGVAGLAEMLIQSHEKEIHILPALPAAWPSGSVKG
                     LKARGNISVNLSWAGGKLNLAEFKGAADGLVEIKYAGQIKKLNVQQGKWISLTF"
     gene            complement(20139..20477)
                     /locus_tag="PKM27_11925"
     CDS             complement(20139..20477)
                     /locus_tag="PKM27_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNR08017.1"
                     /translation="MTIAELQHEVDQWIKNHGVRYYSELTNTAILAEECGEVARLMAR
                     IYGDQSFRTEGEADKAAQSLAAEMADVLFVLTCLANQCGVDLQHAIAVNMEHKKIRDA
                     ERHRHNPKLK"
     gene            20653..21027
                     /gene="crcB"
                     /locus_tag="PKM27_11930"
     CDS             20653..21027
                     /gene="crcB"
                     /locus_tag="PKM27_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNR08018.1"
                     /translation="MNWIAILSVAVGAAAGGVLRHLLSVWMRADMARFPWHTLIINLS
                     GSLFLGILWAYFLKHPGNLALRLALTTGFCGGFTTFSTFSLEMINLFQKGQYWSGGIY
                     LFSSVLGGVLLAGLGFAMMGKS"
     gene            complement(21124..21909)
                     /locus_tag="PKM27_11935"
     CDS             complement(21124..21909)
                     /locus_tag="PKM27_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR08019.1"
                     /translation="MNRRYRDFRSDHLERQVALSFYHGPAPDRPKIDHLVIVNDGQEL
                     AQMDMGQMVEARAVHHRRSILVVGCAAAPGEARKQEYGVQWSSDYAGRGHLAAAYNQF
                     ILSELIPFLHQEYSLDHHTDTTIAGWSLGGLSAMDIAWHSPHRFRKTGVFSGSFWWRH
                     PEKIKDDVHHRMMHHRVRNTSSRPELKFWFQAGTEDEKADRNNNGIIDVIDDTLDLIN
                     ELHGKGYHREHDMKFHIVEGGRHDVPTWKKMMPVFLDWVVTGD"
     gene            21951..22904
                     /locus_tag="PKM27_11940"
     CDS             21951..22904
                     /locus_tag="PKM27_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08020.1"
                     /translation="MKKIGILHGMESSFPPAFVDRINAMDVPGIRAEAVKIDRVIQGE
                     PLQYDVIVDRISQDVPFYRAMLKNAAITGTAVINNPFWWSADDKFFNNCLAVKIGVPV
                     PKTVILPSKERPPDTQETSFRNLAFPLDWQGMFDYIGWPAYMKPFAGGGWKHVYKIHD
                     QADFFDKYGQTNQLVMLLQEEIVFDHYFRCYCIGSKYVRIMAYEPRNPHHLRYDVRLP
                     VNSEKLISLIESYVIRLNEFLGYDFNTVEFAVRDGIPYAIDFCNPAPDADLHSVGKDN
                     WDWVVEHAALYAIERARVHKPGQDNLTWGKYISRSVNKEML"
     gene            22907..23761
                     /locus_tag="PKM27_11945"
     CDS             22907..23761
                     /locus_tag="PKM27_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR08021.1"
                     /translation="MWRILLWSISLIPAFYSYGTAQIPPKDWVKLFNGKNLEGWNVKI
                     HHHDYGVNFGNTFRVKGRKIVVAYDQYGDFNNQFGHLYYHKPYSHYRIKLEYRFRGSL
                     QPGAPDYTLLNSGVMFHSQDPATMPREQDWPISVEMQFLAGLGDGKPRPTGNMCSPGT
                     DIVYQGKKYPGHCLNSSSETYDKEKWVEAELEVLGDSLVRHRIEGKVVLEYTHPTMGG
                     GVANRFDPAVWEPGKALTSGFIALQSEGQPIEFRKIQLVDLKGCKDPGAKNFRSYLEA
                     HDPSSCIY"
     gene            23857..24693
                     /locus_tag="PKM27_11950"
     CDS             23857..24693
                     /locus_tag="PKM27_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HNR08022.1"
                     /translation="MYIKERESIRIAIVDLYNGEANEGIRCIHELIHSFSLEINLEVI
                     TDTYALRLEEDVPDLSYDIYISSGGPGSPLDTKGMAWDTKYFELIDSIEAWNRLPHAR
                     KKYVFFICHSFQMICRHYGLAEINERQPSSYGIFPVKLTAAGRAEALFETLPHPFYIA
                     DFRMFQVIRPDMNKFLSHGYEILAEENQPAPGTEDRALMAIRFSDYFVGTQFHPEADP
                     EGMLVHFNGAKKAPIIHNVGETAYHEMVVHLKDPDKIARTRATILPNFLRMAATQLEL
                     VR"
     gene            complement(24767..26344)
                     /locus_tag="PKM27_11955"
     CDS             complement(24767..26344)
                     /locus_tag="PKM27_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08023.1"
                     /translation="MNTEEFDDIIRNKVTQPAASEGPRDWDLFMLSAIDKGEEKIFED
                     HPTDRVVNEALGEFKADYNAGHWDLMARKLDEEVPDQPVVHARFDKTVSNSLKGITRK
                     YDASTWPKLAARIEAEEKYLRHYYRAKMIEAVLFCLLVLTLFQISRSGKFTPPFIKTA
                     EPETALRNNSNHDGDQAIATLYPKTENNTSIALNNSSGNTTGTNSPGALSATTSGGMD
                     LTSASRPVVARHDVLLERISSRQSLIGSGQIYSRLDGKVKSLPLLQELSGEPVEDGEE
                     ALLAHQLVPVPEPSSLELPDVVKLTVKGGWKLGMYSHFDYNQIYLPDQQFLVFGKKSD
                     LYPATTVSSTGYGAGFKMHYLKNRFGLEFGLGYANRSYSPSRAYYLDPYWNINFKKIE
                     YDIVALPIAVRYTTKSNTRLKPYAMAGINANFIAKAIYDVLPEIKQVNFNGERKLNAV
                     QNSIYSRVQDQFTHLDRRRALLYGQGSLGLEWKVNSSLDVYSQLTYAQRFLNRQFGPN
                     WDQFRSLSLEIGLKTGL"
     gene            complement(26334..26885)
                     /locus_tag="PKM27_11960"
     CDS             complement(26334..26885)
                     /locus_tag="PKM27_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNR08024.1"
                     /translation="MIPLQSEEEFIKACSRNERWAQKKLYEDHYGIMMGLCLRYANND
                     EDALDIMHEGFIKVFKHISKYQPGTSLSAWIRRIMVNTSIDYYRKENRRRTENLETAY
                     AIGTDDVDAISQCTEKEILTAIQELTFAYRSVFNLYVLEGYSHREIAEQLGITESTSR
                     SNLVKARIKLKEILLGKGKLHEH"
BASE COUNT         6616 a         6907 c         6773 g         6591 t
ORIGIN      
        1 tgactgttga ctattgacta ctgactactg actactaaat cagatcaaca atggcaaacc
       61 aggtggtcag gaatgaaaac agtatgaccg cgatcactcc cagattcatt ttccatccac
      121 tggtgatccc tttcatggtg ttttgacgat taagcaggat caaggctgga atggccacag
      181 caggtaaaat gcaggcctgg aaagcctgtg agaatatcat cagggcgggg gctccttgtt
      241 tcagaaccag ggtcccaaag caaaatacca gcccaagcag gatcaaccaa cgatacaaag
      301 gcgacttcag gtccctgggt ttgcccagat aatcgctgac cagccagggg gcaatcaata
      361 cgatcggaaa aatagtggac aggcctgctc cggtcacccc catgatcagg attaaagcgg
      421 ctgctttccc gcccaggggt tcaaacaggt gaatcatctc cacggtattg ttgagcctta
      481 agcccatgac atgaagggta cctgccgaaa ccgccatgat gatcccgctc aaaaacaaca
      541 tcataaaggc cgaaacgaaa gcgtctcttt tctccgccgg gagatctttc gccgtccagc
      601 ctttttcggc caccacggta ccgcgaacga taaacaccgc cgcggaacag gtggtacccg
      661 ccatggcggc gataaggccc agggccccgg gctggtcggg tatatgagga accattccct
      721 ttaaaatggt tgcaaaatca ggcttaagca ggataaaaac caggacaaaa cagagaccca
      781 tcaaaaccac aaaaccggtc agcactttct caaattgttt gtaccgccca aaccacaaca
      841 agaggtacat cagcaccaca atgaccagga taatccaacc tgtgtgaacg accgtcccac
      901 ccgatacgat tttaatgcct tcctgtaaga ggtcggctac aataccggta accccgatca
      961 gggcaaacaa ctcccccacc accagggcaa ccaaaacata cagtgcgagg atccaaccga
     1021 atttaaattc atgcttaaaa ttataaagcg gggtcctgcc ggtcaccagg gtcagcctgc
     1081 cataagccac catcaggata taagtgaaga cgcaggacag gaccagggcc cagaactggg
     1141 acattccatg gtccgcgccc gatttggcca tggtcgtgac actgccggtc ccgatattgt
     1201 aaccaatcaa aaacaggcct ggtccgaccg cggcgagggc aatggagatt ttcttaaaca
     1261 ggtttttcat gatggcggga tgtggcttta cgtcgtccgg caagtccgat tagggatgat
     1321 tgaggtcaaa cacctttttt ccctggacgt aggttttttg aatggtcagc ttaaaatctt
     1381 ccagggtaaa caaaaccagg tcgccgcgca gacccttttc cagcctaccc cggtcggtga
     1441 ggccgtataa ccgggcagga atggtgctgg ccatttgaac cgcttccgcg agcgagcagc
     1501 cggtcacctt taggatatgg cctacgccct tggtaatggg tgaagccgat ccatacaata
     1561 cattttgcgc gggatacctc agcatgcctt cccgggtaag ttcgatcgtc tcgccggtct
     1621 gggttttgta ttctccgggc tcaagggcgg cataactggt gacatcggag gtaatgatcg
     1681 tcctttccgg accttttacc ttgtagaaaa cgcggatttc ttcttcattt aaatggaagc
     1741 cgtcacagat gatgctgatc aggagatcct cgttggccag ttgcggccat aagggattga
     1801 gatgacggtt gatcatattg gcacaaccat taccaagatg cgttgcaatt cgggctccaa
     1861 gaccaacggc ttcgtccacc tggctccggt tggcattgtg atgccctata gccaccgtga
     1921 tccccagttc cttgcatttt ttgataaatt ccatggcccc ctccagttca ggggcgaggg
     1981 taacctgcaa gatcttcttc ccggaagcat ggtacaattt cataaactct tcccagtccg
     2041 gaaggcgcac ataagctttg ggatgcgctc cccggtaacc gtcttccgga ttgatataag
     2101 gtccctcgag atggaagccc gggatggacc ccaacaggga gtctcccgaa taattctggc
     2161 ccagcacttt gaagttcttc atcagcactt cgtggctgtg ggtggtcagc gtgggcagat
     2221 aggtggtcac cccttttttc cataattcac gggttgcctt caggatatcc tccggtctca
     2281 gatcgctgtt gccaaaggag aaggaaactc cggcgaagcc gttcacctgg ttgtcaaaaa
     2341 aacccggcgc gatatagacc ttcgccggac cggaccattt tttgattttt ttgattgact
     2401 caatgcggcc acccttaaac tcaagccgga ccggttggcc tgtggcatag tgtatacctt
     2461 caactgcctc ctgggcctac cctttaatga tcatggccca aaaaaatacg agcaacaggg
     2521 ggaccggaga agaaggaatt cgattgttcc ctgatgcgct catccttcga tattgaggta
     2581 ctccacatcg gtccattttt cgctcaggac tgatttttca agggcttcca ggacggatat
     2641 cccgttcaat atactctgtt gtgacctggg ttctttgccc gtcgcaaaca tctggaccat
     2701 atcggcgtct gccctgggcg ggtcgctttc ttttacttta ggtttgagtt cgaccatggc
     2761 tttgctggtc tccacatagg tttcccagcc ataggctttg tttttaaata ataaagtggc
     2821 ggtcaggccg gaggcgaatt tgaggagggc atttcccgcc ttgccgccac gactgacttt
     2881 tacagcctgg atgtcttccc caaaaatcac catcagcggc tccaccatgt gcaccccgta
     2941 gaaaaagatg ccgccataag gagagtccgg gtcaacgggg ccggtgatga tcacctggct
     3001 gatatccggc atcgccttca cctgggttct caggtccaga gtggcgtcgc tgtgggcaac
     3061 ggaactgtat gaagtgaccg gagttccctt tttcctcgcc atctggagaa aggccaatcc
     3121 ttccgaagcc cggtaacaaa agggtttatc gatgaaggtg gggatcccgg cttctacgaa
     3181 tggccgggcc ggttcaagat ggtatttggc atgacggtgg tccacgatca gggcatcgat
     3241 cttgcccagc atctctgccg tctctttgac ctggatggga atattccccg ctttcatgga
     3301 tttttgtgca tattcatcgg tttctcccca aaccatggtc acttccactc ccggaaattt
     3361 tttatcgata ttgaaaagtt ttccgaaggc ggcagtatgg gaattttccg cgccgatgat
     3421 gccaatgcga atgggcttgg aagtccggaa tcctgaaaat gcctttaggt ccggtatgcc
     3481 ggaacttgcc agcaaagccc ctgttcccag ggtatttaaa aatttccttc ttgatttcat
     3541 ctcttttggt tacggataat aaaaaaatga cttgaaagag ccgaacggct ttccactaca
     3601 atttcatttc ccagcccttt tcatattccc tcgaccaaaa cttttgagcc cccgcatcac
     3661 ccaggatgtg cccattctcc gggttgatct ccagggaatg gccgactctc tgggcaatat
     3721 ttcccaactg caccagcagg gtgcttttat ggccggaatc aatatcggat ttcagttttt
     3781 cccctttgga aatatggtta aaaaaattcc ggatgtgcaa ggcgtcgaga tgtcctgccg
     3841 ggtttgcaag gtctcttggg tccacgggac ggctgtcctt tttttccttc accagtttat
     3901 tcttcaggtc gtaaaccctg tattcatttc cgctggggat cattaaggcg cccgtttcgc
     3961 cataaaaaat caccccgacc gagctgcctt cgatatgcct gccgttgcaa cttctgcctt
     4021 cccagctcat cgaagaacct tctttaaagt ccaggttgat gacctgggta tccggggttt
     4081 cccaatcatc tttaaaatgg tacttgccgc cattggaact cactttcacc ggaaagtcca
     4141 ccccaagtcc ccagcggagc aggtcaatca tgtgggtccc gttgttgagg gcttcgccgg
     4201 taccccagtg ccaaaaccaa tgccagttgt aatgcaccac attgtcctgg tagggttttc
     4261 gcggtgccgg gccctgccag agttcccaat ccagccagtc cggaatggga gctggtttgc
     4321 cggtgccaat ggactccctg ttgttggtat accagccttt gccgaaatag ggcctgccga
     4381 tgatgccgtt tttcagttcg gcgatgccct ccatcacgtt gggccaggat cgacgctggt
     4441 tgcccatctg taccgacttt ttatatttta gggcagcctt caccagtatt tctccttcct
     4501 gcgggttatg gctgcaaggc ttttcaaggt atacatgttt acctgcctgc atagccaaca
     4561 gggccgcggg cgcatgccaa tggtcggggg ttgcaaccac caggacatcc acctcactgc
     4621 tctccaggga tttcctgaaa tccccatagg ctttgggttt taggtcctga atgccttcca
     4681 gccctttaat gcatttggct gaagcccgat ggtccacatc gcagacatga accacgtcgc
     4741 agtcctcctg ataggcaaaa ttctgggaga gggcaaaacc cctgctgttg actcccatga
     4801 ctgaagccct gattttttca ttggcaccaa ggatcctccg gtaactcgag gcggaaaaac
     4861 caggcagcac tccccctgcc agcaagccgg ctccggctac agtgttccgg aggaaacggc
     4921 ggcgggacct tgaagattta gttttcatcg tcattgatct tatgtttttt caagctcctc
     4981 aaaatagcgg atagtttggt aatttattat aaaaaaaagg taatagggtt gatgcagata
     5041 cggatcaagc cccttcaaaa gcctccatgc cgggatcaat gcagcagcgt cattccaatc
     5101 agggacaaaa ccaggaacag gacgcccacc caggctccca ggtatatttt tttctgcatg
     5161 gcctggactt tagctcctaa ttcagggctg gggctttgtt gcatttggac gaggagcgcc
     5221 ccgagttttt gggcattcgg ggcctgtatg aaggtccccc acaaaaaagc gataacccct
     5281 gagatcaccc cgatcagcag tccgatgcct cccggccaat gcaggctcca ttgtttaaag
     5341 cctccggtga tccgccaata aaggagggta cccatcaaca gggtggtcca ggaggccagg
     5401 ttcatggcca ggggcagctt taaatcacgc atgaggtggc ccataaaaac gccggcggac
     5461 ggtcctgttt ttttcgccgc aggcaataga aaagcagcaa tcatcgccgc gcctccgagc
     5521 caaaaagtgc cgccaagaat atgcagtgct ttgatcagca tccagatgga aaacatggtt
     5581 cttcgtattt attttaccaa agtatgtaaa attagataaa tcggattaac caggatgtac
     5641 gaggtgtgag acaggcataa ggggatcccg gatgcccccc tttggggagt tcggtacgtc
     5701 catgaaaact accttgcctt gcgggaccct ttctgcctga aaggagggag tagtttccgg
     5761 aggagtgcat agattttgtc ttccctggtc ttgttttcct tctccaggcg gctggccagt
     5821 cgtgtcagat gcccattcac cgatccggag gaatgttggt aggccttgat ggatttgatg
     5881 agtaaggcct cgtcgtgcca tttgccgagg gcgtcataca taggtttcaa ctttctatcc
     5941 accgccttcc aatcctgcga cggttttatc ctcagaagga tttccaggct ttcctgtacc
     6001 gttttcagta ttatgcggat atcgtggagg gttttgacat cccggggttt ttgggacaag
     6061 gaataaacac gccccaggag acgttggatc agggaagctg cgctctgcgg tatggagccg
     6121 ggatgaatgt tttgtatttt attgaccagc tcatgattga gtttatcgag ttgtttaagg
     6181 tcaatgacca gggtttccgc atgcaaatgg cgggttttta ctttaaggat ccggcccagg
     6241 tattttttat aagggaccag gtacgcagtt ttttttgatt tcagaagccc aaggctgatt
     6301 tggatatccc tcacttgtcc cgcttttctg aataaggggg cataaatctt caaatggtcc
     6361 cttttttgca gggcgcctgc acaggccttg tccatcaaca ccaggaggga gcgaattccc
     6421 ttgatggcca cccttagccg gtgtacgttt tccaccgtct ggctgactga ggtttttgcc
     6481 aggcacctgt agaacagatc catctgccgc cggaaccggt ttgttatttt attgccagcc
     6541 acgattaatg ttcgtttggt tgaatggatg ctcgtacata ttcctgttcc gtaacgatat
     6601 taatatcctt tgacccacaa cggggcgccg tcctcccatt tgttatgggt aagcctgaag
     6661 gtctggccgt cgtccaatct ttttgcaaca atttccccat agagctgagg gctgaaaaag
     6721 aattcctggg acaggggttg ttcatcattt attccaaact cctccgtggt ataaataatg
     6781 tacaatccat ccggtgaaaa atggggatgg gcttcctgtc cttcaaaatg ggtgatttgt
     6841 tttgatgcac cccaggaccg atcgggttgt ttttccagga aatgaatatt catggtggtg
     6901 attgtgctgt cgacacgctt tccgttaata tccgaacaat aaactacctt attcccatcc
     6961 ggggaaaggg caggaaaatt ttctctccaa ggtgaatgag tcaggttttc aatttgatct
     7021 tttgtcttga ggtaaatatc aaaattgccg gtgtgccccc cgcggaacaa catggatgta
     7081 ttatcggcac tgaagtccgc aaacccctgg ttggatccaa aaagggatat ttttttaagt
     7141 tccttatcca tgaagcggta ctcccagatc tcgcccggcg ctgagggccg gttgcgaaaa
     7201 agaccagcgg taaacaggag cgaggatcca tccggcttgt acttgatatc caggatctgc
     7261 gatactttag aatgtcccgg cttgaaggta aacaaccatt caaccgctcc cgtctcatgg
     7321 ttgactttta aaaaatcaat gttcaaatta aacaggttgg aataaaatac aataaggtaa
     7381 accagcatca tgcagaggag gccagtccca aagcccaacg ttctatatac gatttgaaat
     7441 gggctattta gaaccgatgc cttccctccc ttcctgaaaa aataaaagca aaggatcaaa
     7501 aatggaatcg ccagcagaag aaaggtggtc ctgctcctgc tcaaggaatt cgaactggca
     7561 aagatctcat aaccggaaat ggtcaaaacc gcgatcgaaa ggatcagcag ggccgacatg
     7621 gctaggacgt ttttccaggg tttcaccgat aaccgatctc taatggcccg gtataaacca
     7681 aaaaaaatca acaaaagaag acccgcagga aacaataata aaacagcgct gtatttggtc
     7741 agcaagacct cctgtatgga tttcgactgt tccgctgcaa agaccaggga tggtgaatgt
     7801 ggatcggggg gcgcggcaaa ggcccttcga accccatata gtcgaaccgt tttctccggt
     7861 aatgcatatt gttccatggg tcccctgacc aacaaatggc ctgcgaaccc gcctgcattg
     7921 gttttttctt ccaggggaat tttacctcca tgaaaagccc ttaaatcctt ggggccggca
     7981 accataccat acatggccag gctatcccgg taataaattt ctgtatttaa tgaatttaaa
     8041 tttaaaacca taaacttaaa tgctcctcga tcttcctggg tccagtgtgt gaacaccagc
     8101 aggctgtcat tcaagggaca gggagagatg gccttcaggt caacagggga tattcgtttt
     8161 tgcgaagaac catctttatt catctggtat atctgaggca ttccggaacg aaggctgtaa
     8221 aaataaattt gctttccgtc caaggaccat ttcgggctac catcccaggt ttcggtcctg
     8281 gtcagtcttt ccagcgcaga accgctggta tccatggaat agagatttcc ggttcggtac
     8341 atggcaaatg gaaattccgc atggggtttg accgcatggt cgcggtcgct tgaaaataca
     8401 agggttgatc catcgggtga ccaggcaggc ctgaaatccc cgccaggatg attcgtcaga
     8461 tttatcgcct gccggatgtc catcacactg tccggaacaa aaggcaattt ataaatctcc
     8521 gcattgcctt gatgggtgga gacaaaggca caccatttgc catcgggaga aaaaacaagc
     8581 tgatcctgca tggatgcgct gttgaccagc aggcgcggcc gttcggcctg gtctatggac
     8641 tgaatataaa gcctgggcat tccttcacgc tccgaggtga aaaccaacca ttttccgtcc
     8701 gggctgatca cggggtcata atccagcgcc ggatcctctg cgatcggttt gaaggacttg
     8761 cctccatccc ccgacaggta gatatcccag ccttccgggc ggaaggaaga ataatagact
     8821 ctttccggag actgtccaaa aagggtccat gccttaaaaa taagacagga ccagaggatt
     8881 aaaggcttaa gcatgctttg aatttaataa aatacaatac gtctgatatt gtactcaaag
     8941 taataagaat aaactcaaag taaaattttc atatcccggg gcgatgccct tgtaaacttc
     9001 aatgttcatg gacaggagcc atgatcgaaa actgttcatg gatcttatgt ctttccccta
     9061 ccagccagac gatgtcccct tcttcaaaca agagatcgga ccgtggattg atcatgcgct
     9121 ggcttccccg ctctatcccg atgaccagcc ctttggtttt ctcacggatg cctgaatttc
     9181 ggatggtctg gcccgccagc aggttgcttt catccacctg aattttctga agtacgatgt
     9241 cgtcgacatg gggttcagca ggctcaatgg attcgcaggt atcaaaggcg ggtttgaact
     9301 gctggatttg ttcatcggtg gcaatgatgc ccagttgatc atgagggtaa atcttttggt
     9361 ttctgtccgg cgcgtagatc aatttgtcgc cacggcggat ataggccaca ttcaccccat
     9421 aatgctccct ccagcccaat tcctccaggg atttgcccac gctgtaagaa tggggatcca
     9481 ccttgaggtc gatcatgtgt gcatcccagg cggacagttt aagggaggaa gtaaatttct
     9541 ggtgcaaccg tttttccgcg gtcaggtttg cttttgcggc ggattcccgg gcattgagat
     9601 tatccagaaa ccggtcttcg agcctgtggt acaggtcctg gatcttatgg gcccaaggca
     9661 gtaaaaatcc caggatcagg ggaactgcga tcagggatac cagggttgaa aaaaattggt
     9721 caataataaa tccgatcagc aagacccccc cgatgatgcg taaaatatct atgatcctca
     9781 gggcaccaaa gctcgcggaa cggttcagcc acagttcccg gtaagtcata tttcccggac
     9841 gtttggccat cagtccccat aaaaaaggca aggacaccgt aagggtcaga cccagcgcaa
     9901 tgccccgacc gaccacttcc cggtcactcc attggatcag caaaggaagg aggtacttta
     9961 cggaaagaag caagatcgcg gccaatacga tcaaattgat caaaatgata ttgacatagg
    10021 attttgcggc gacctgccag gcaccttcca cctggctgac ctgggtactg taggcataac
    10081 ggtccagggc attcacccag cgtttgggca ggatccttgc aatcaggtca taggctttat
    10141 ccgcataccg gatgagatag ggtgtggtaa aagtggtgat cgcggaagcg cccaccgcaa
    10201 ccggaaacag gaaatcactg atcaccccca gggaaaggcc cagagtggcc acgataaaag
    10261 cgaattcacc gatctgggcc atgctcatcc ccacctgcac cgattgtttc agggtctgcc
    10321 cggagatcaa ggctccggcc gcggtgctga aaaattttcc aaagatggtc aacagggtta
    10381 ccaggatcac cgcccagccg tattctttta tggccagggg atcgatcagc atcccgaccg
    10441 aaacaaaaaa aaccgccccg aacaaatctt ttacgggttt gatcaggtgc tccaccttct
    10501 cggccttggt tgtttccgca aggatggacc ccatgataaa agcgcccagt tccgccgaaa
    10561 agcccacctg ggtggccaat acgaccatac ccagacaaag gccaatggcc aggatcagca
    10621 gggtttcctc atccagcaaa gtttttaccc tttttaaaaa ggtgggcaat atgaaaatgc
    10681 cggcgatgaa ccaaaggatc agaaaaaaca acaatcgggt gatggtcagg aacaattggc
    10741 tcccttcaat ggtcctcgtg acggccaccg tggacaggat caccataagc aggatcacca
    10801 cgatgtcctc caccacgagc accccaaaca ccacccgggc aaactgcttg gtcttgaccc
    10861 caagttcatc aaaagcgcga atgatgatgg tggtggaaga gctggccagc atacctccca
    10921 ggaaaaggca gtccatggtg gtccatccca tcagcttgcc ggtgatgtat ccggcaaggg
    10981 taatgaatac aatctcgata ccggcggtga tggaagccgt gcccccgatg cgcatcagtt
    11041 ttttaaagct gaattccagg ccaaggctga aaaggaggaa gatgacgccg atttcggcaa
    11101 gggtttccac attctccata tccgccacgg tgggaaatat agagaggtga ggccctacca
    11161 gaaatccggc aaggatataa cccaggacca ggggttgccg gattctcctg aacaggatgg
    11221 ttaccatggc ccccacgatc aggatcaggg ccaggtcttc tatcaatttt ggtaaatgtc
    11281 ccatagcaat ggttcaaata aatgattttc attaatgcag gatccaatgg atctggatat
    11341 ttctccggat ttggaggtga ttaaacccag gaactcaatc gttgggccat ctatgcttcc
    11401 ggctctttag gttgccgggc acgcaggatg gctgcttcca tttctttggg tttcctggta
    11461 ccgatcagca attttttgtc gtcgttaaat tccagttgca gcccctggtc cccggaaatc
    11521 gacagcgctt tgccttttcc aaatatccca agcctgaatc cccatccgcc gtattcgatg
    11581 attgcattgt atttgcggac ctcccatcgc cgtatggaat tccatggata tcgcctgggg
    11641 cgcaggtgaa aaggaaaaaa ccgcacctgg acaccttctt tcgttatttc cgtatccaac
    11701 cggatgttgt aaatgagcac ggcaaaaaat agtataagtc ccagggtcaa cagcaaaacg
    11761 gcatccgtca tcggtttttg accaaaaggc cggccgacga gcacctggct gatcacccca
    11821 taagtatata atgcaatcaa agccaacagg atcagccaga cccaccattt tttaaaccgc
    11881 tgcatctccg aaaaaaatac ctggttctcc attttcgcca aaataaggga atatgaggag
    11941 aaatggaagt tagctagtta gccagttagc tagttagcta gttggcaagt tggcaagtta
    12001 gtaagttggc aggtcgccaa gtcctttagt atgaacgatt gaagttatcg gattcagtcg
    12061 cggatcaaaa aaggtttcac ggtttctttc cagatctcat acccttccct ggaaagatga
    12121 aggcggtctt tgataaacaa gcgttccggg ggcagcccat ccggtcccag catgggggta
    12181 aatacatcgg cgtaggccag gtatttcctg gatttgatat agcgccggat gcggcgattg
    12241 gcatccacga ttttctccat aaggtgtatc cgcgaagggc ttggtttgag cgcaatgaaa
    12301 aggatttttg tatccggcag ggaatcgcgg atgattccgg tcaggctttt aaatcgttta
    12361 aacaccgttt tcccgctgat gctgctgtcg gcagcgaggt cattttcccc gcaataaatg
    12421 accacttgcc cgggctggta tttgagaatg acttcaccgg cataccggat gacgtcgggc
    12481 agggtggatc cgccaaaacc gcgattgagg acccggtggt cactgaaatc ctgttccagc
    12541 gttttccaca tccggatgga agaacttccg gtgaaaacga tcattcccgt tcccggccat
    12601 tgttgttcat cctgccggct gaacgcgagg atctcagacc aaaacggggc ttgctgggcg
    12661 atcaatccgg cctgcaatcc aatcaggacg gctgcaaaaa tgaaaatcct ctgcacgatg
    12721 aaggtccgat taatttaaat ccattttaat ctccatgccg gtgtctgtca agccttcttg
    12781 cgtgccaaga cccgtttaac ggcttttaca atatcctgca ccccaagacc gtacttgatc
    12841 agcagttcat cgggtttacc gctttctcca aaactatcct ggacagccac aaattcctgg
    12901 ggcaccggaa gattgcgggc cagcacagcg gctacggcat cacccagtcc tccattgatc
    12961 tggtgttctt cggctgtaac cacgcatttt gttttacctg cgctttccaa aagagtggat
    13021 tgatccaggg gtttgagggt atgcatattg atgaggtcgc aatgtattcc ttccagggca
    13081 agcaatttca ccgcttcgac cccgttccac accaggtgtc cggttgcaac gagggtgaca
    13141 tcggaacctt cattcagcac ctgggccttt ccgatttcaa atggaagggc ttctgtaaaa
    13201 atgggccagg ccggacggcc aaaacggagg tataccggcc cataatgttt cgaaatagcc
    13261 accgtagcct ggtgggtttg ataggcgtcg caggggacga tcacggtcat gccgggcagc
    13321 gtccgcatca tactgatgtc ctccaggatc tggtgcgtgg cgccgtcttc ccccagggtt
    13381 aatcctgcgt gggatgcaca gatctttaca tttttatggg aataagcgat cgactgtctg
    13441 atctgatcgt acactctccc tgtggagaag ttggcaaaag ttccggtata ggggattttc
    13501 ccggtgatgg ccatgcccgc cgcgacgccc atcatattgg cttcggcgat ccccatttga
    13561 aaaaaacgtt ccggaaactc ttttttaaat tcatccattt tcagggagcc catcaggtct
    13621 gcggtcaggc ctacgacctc tttattttga cgggcggctt caagcaagcc ggtgccgaat
    13681 ccatcccggg tggccttttt accggtgacc atcaggtcgt tgatattgat catgtgtttt
    13741 gctatgcttt ttaatgatat ttaataatct cccagcgttt ccggcagttg ggcgagggca
    13801 tttttacatt gttcttcatt gggggccacg ccgtgccatt tgtggctgcc catcataaag
    13861 tcgaccccgt accccatcac ggttttcatc aggatcacca ccggtttgtg ttgatgcaac
    13921 ctggattttg cttcatcaag cgaccggacc acttcctcca tatcgttgcc gttttcaagg
    13981 agaaggacgt cccaaccgaa ggcacgccat ttggcaggca ggtcacccag gttcattact
    14041 ttttctgtgg ggccgtcaat ttgttggcca ttccagtcca cggtgacgat caggttgtct
    14101 acgccgtggt gtgccgcgct cattatggcc tcccaattct gtccttcatc caactcacca
    14161 tcaccggtca ggacaaaaac aaggcccggg tctgaatcca gttttttagc cagggcggca
    14221 ccgaccccga ccgagaggcc ctggcccagg gaccccgagg ccacgcgtac accgggcagt
    14281 ccttcatgcg tagcgggatg cccctgtaaa cgcgtgttta tttttcggaa agtggccagt
    14341 tcgcttaccg ggaaaaaacc gtgcctggaa agtacgctgt accataaggc ggagatatgg
    14401 ccgttggaga ggaaaaacaa atcttcgttt ttcccttcca tgttaaaagg aaggttatac
    14461 ctgaggtgcg ccccgtaaag cgctaccatg tattccgtgc atcccagcga gccgcccggg
    14521 tggccgctct ggcactgata taccatacgg acaatatccc tgcgtacctg ggaggcgagg
    14581 gctcgtagtg aatccagatc ctttttcata aactccgcaa agaaagagag aatttgatta
    14641 acctcaaaca aaggcttgcc tcaaatacaa aaggcaggta taaaactaca cctgcctcct
    14701 gttcattcca gtgggttcat cggaaccccg ggaaatcttt tatttcactg cagtcgaaag
    14761 ttctttgcca gctttgaatt tggccacttt acgggcagca atttttatcg ttttgccggt
    14821 ttgaggattc cggccggtcc gtgcggcacg tttggataca gagaaagtgc cgaaaccaac
    14881 caactgtacc cggtcccctt ttttaagggc tttacggata gaatccagtg tggaaccgag
    14941 ggcggcttca gcctgtactt tggtgattga agcgcctttg gcaattgcat cgattaaatc
    15001 tgatttgttc attttatttg aattttgatt aaaaatatag cgcaaatata tgatcaaaat
    15061 tcatatatat cgcatttttt aataagtttt ttttccggga aggccctgtt ttcaatgctt
    15121 cagggggata agccgtccga aaggcctggc tgacaaggat ttccaggttt accacccttc
    15181 ccgatgcgca ccattcctgc ttttctcatc caggatccaa tggagggaga gcaagaggat
    15241 aaacatcgcc aagccgggaa atacggaaag ccaccaagcc ggcagatatt gccggccttc
    15301 attcaggatg ctgccccagg tcacactttc cagttttaag cccaagccga taaaggacaa
    15361 ggaggcttcc agcaataccg cggtgctgac tgaaaacaag gccgtgacca gcagggtgga
    15421 ccggatgttc ggccaaatgt ggcggctgag gatccggtac cacggaaggc ccaggttttc
    15481 agaagccttc acataattca gcgcactttc ccggagtgtt tcagcacggg ccacctgggc
    15541 aaacaacggc cagcgaataa aagcgatcag tacgatcaat aaagcgccgg atggccgggc
    15601 cagaaatacc agcagggcca tcatcaggaa aaggcgcggg aaggattcga aataatcgat
    15661 gaaacgcatg aacaggctat cgacgggcaa cgaaaaagtt ttgagcccgc tccgcctccc
    15721 cggtttcaac catttgctgc atacccatat aaacaaagcg gtccaggcaa ccaacaaaac
    15781 catgcggacc atcccgctgc ttaaggaccc gatcattaaa aattcatata tccataaaag
    15841 gaaagacaag accaggccaa ttccctggaa cagcgtcaat ttaagtttat ggtctttcca
    15901 atacccgatc gccatacccg caaaaatgcc cgggatcccg gcaagcaggg tggacaataa
    15961 accgatcagc caggcataac gggtcccgtg gatcatcccg gcaagcacat ctctgcccaa
    16021 ggcatccgtt cccagccaat gttttttccc atgcagggcc caaccggctg aaggacttaa
    16081 atagcctgag ccatccgggt caatggtgga aggagaatag ggaaccggtg cagggagccg
    16141 atgggtgcaa tccgtatatt caaacgcagt ggtcttccgt aacaggccgg tatactccgc
    16201 ataatccctc caggccggaa aatgccagcg gccctcacaa atacaaaaca gcggtttttc
    16261 attggcaacc aggtttgcga tggcggcaat aaacaaactg ccaacgatca ccaacagcgc
    16321 ggcccggtag gtcgcggact tgttttgcca atattgtgca aacctgattt tcatgaggcc
    16381 ctccatcgga tcctgggatc ggtgaaccca tagagcaggt cggccaggag tttgcctaaa
    16441 atggtaaagg tcccgatcag taaaagaata ttgaacacaa tcaaccagtc ctggatcaaa
    16501 atggattgca gcatcaacct gcccatcccg ggaatattaa acaacacttc tatcaccacc
    16561 gaccctccaa acagggcggg tatctccata cccaacagag caatcagagg caggagggca
    16621 ttccggcgaa gatggcgccg ggtgatgaaa tcttctgcat cgcctttgct ccaggccatg
    16681 aacgcgtaag gtttgttttt ttcatcccgc aacctgatgg aatagatccg ggtaagataa
    16741 gccagggaag ggagcaccag gcatatcacc ggcaagacca gctgggcgcc ataccggctc
    16801 cattgttcca aaagagaggc ttcaggattt gaatcgaaaa aaccgggact ggggaaaata
    16861 tgcaaagccg gcgacagggt atcgctggca aaggccagga tcagtaaaag cgccagaaaa
    16921 aaacggggaa tggaatacag aaaaaagaga accgattcga tgatctttcc tttgtgttgc
    16981 tccttcctgc gaaacaacca ttcgcccaga aacaaggaca gcaggtaaac caccagaagc
    17041 gaaaccaggt tgatggacag ggtccaggaa accgcttccc tgattttgac ccaggccggc
    17101 cggtaatccg acctcgaaac cccccagtcc ccgcgcatga tttgattgag ccagatatga
    17161 aaacggttat ggcttccatg ccagccggga acgggaacga acaagggaaa ccatttcctg
    17221 ctcttctgca tttcatgcag tgaactttta agtttgcccc aaaggacacc agccctggtg
    17281 gaatccgtca tggcatccaa tttggcagac atggcattta gatcatccag gcggctcagt
    17341 tcttccacca gcaatttatc gagccggtgt gccgcctttt ccaccatctc cctttcaagg
    17401 gtaacataga catggacaga gggccaggga actccctgat ggagaaagcg cttaatccgt
    17461 ttcttttcag atttgatcac aaagcggaaa agggtatcgg gataagacca gggccttatt
    17521 gaaaaataaa acaagggcag atccctcgat tcggccctgg cacgctggat tatttcactt
    17581 ctcctgagtg aaacaggcag gttttgcgat tcctgaaggg ggtcgaaggc atccgtttcc
    17641 atgaaatccc ggaggataaa ggccgtaaag gaaatggcca ggagaagggg cactgaaatg
    17701 agcagccggt tcaggatcaa tcttttcatc gcgctggatc cttggtagtt aaaaagtcag
    17761 cgatatccat ttgccttgtt gcacgttcaa ctttttgatc tgccccgcat acttgatttc
    17821 cactaagcca tccgcggcgc ccttgaattc ggcaaggttg agtttacctc cggcccagga
    17881 aaggttcacg gaaatgtttc cgcgggcttt gaggcctttg accgatccgg atggccaggc
    17941 cgccggcaaa gcgggaagaa tatggatttc tttctcatgg ctttgtatca gcatttctgc
    18001 caggcccgcc actcctccaa aattgccatc gatctgaaaa gggggatggg catcgaataa
    18061 attagggtag gtgcccccgc cggccatgcg gatttcactt tgatcggcag gataataagt
    18121 taataatttc agaaaggcat cgtatgcctg ctccgcgtcc aggagccggg cccacatggc
    18181 tattttccag gagatggacc acccggttcc gttgttggtc cgcttcaaaa gggattgcct
    18241 ggccgcctgg aataaggctt tctggtccgg cgtaatggtg ctgcccggat acaccccgta
    18301 taaatgagaa aggtgccggt gccttggatc ctggtcggcc cagtcgtggt accattcctg
    18361 caaatgcccg tcacgactga cctggtaagg ataaagccgg ttcagggcag accgcaccga
    18421 atccaggaat ccgtggtccg tatccaggat ttccccggcc tggatcatcg cgtggaacaa
    18481 ttcacgcacc atcgacaaat ccgaggtact gccatacaag gttgcccctt tataattttt
    18541 gtccgtgaca tagatattct cgggtgaggt ggagggagaa gtcaccaaaa acccctggtg
    18601 gtcttcaacg aggtattcca ggcaaaagag ggaggcccct ttcatcaacg ggtaggccct
    18661 ggtccttaaa aaagaggagt cccgggtgaa taaaaaatgc tcccagagat gactgaccag
    18721 ccaggcccct ccggactgcc agttggccca gttcgggtca cccgagccaa agtccccgac
    18781 cggattggtc attccccaga tgtcggtatt gtgatgacag gtccagcccc tgcaattata
    18841 aaaagtcttt gcggtgaccg caccggtttg ggcgaggttg ccaatgaagg atagcaaagg
    18901 ctggtgcagt tcggaaaggt tgcatacttc caccggccaa taattcattt ccgtattgat
    18961 gttgaccgta taattgctgc tccagggcgg cctcacctgt tcgttccaga tcccctgcag
    19021 gttgaggggt gcttccggag tgcgggaacc gctgatgagc aaatagcgac caaaattaaa
    19081 atacagttca aaaagtcccg ggtcgggccg ctccacccgg ttttgtttaa gccggaggcc
    19141 gatctctgtt ttttcagaag gtgccgggtc gaggtcgagg accacccggt tatagaactt
    19201 ggaatagtct gcccggtgcc gctgtactaa atcggaatag gaatattttg agatcttttc
    19261 caaaggatcc atggcccctt tgatttcatc gaggccctcg cgaaaagggt ccttgtcaaa
    19321 cccgttgaag ctggtcgcta tggtcaaccg gatttccgcc caggatgcct ggtctatcgt
    19381 gatggtggag tccgtggcgt gtttagggcc cccgccgtgg cggatttcaa gggaggctgc
    19441 aaaacgggtt cccctttggt ccgcgtaaac gatggcattg ttcaccctgc gatagttggg
    19501 ttcggcatgc accggcgccc tgccggccat agtcatccgg tgcgccccgg tgtgtacctg
    19561 gtgttgcagc aggctgttca aatggaccgt aaaatgaagc cttccttccc cacgggtatc
    19621 caaccggata acgatgaccc ggtccggatg ggaggcaaaa acggtacggg tataaagggt
    19681 tttgttggag acatattgga tattcacttc tgcattctgc aaatcgagtt gccttcggta
    19741 ttcgctggcc tcgtggggat ggtggaagtc tataaataaa ttgcccagag gggcatagga
    19801 ttcggaaaaa cggccttgta actttcgcac cagggtatcg gctgcggcat agtcttccct
    19861 gaaaagcgct tcccgcaccg cgggcaaaag ccggaaagat tccgggtttt gatctgcctt
    19921 tacgggttct ccggtccata aagtaatatc attcagagag agtcgttctt cccggattcc
    19981 accgtatatc atcgccccca tgcgtccgtt gcctaccggc agggcttctt caaaaaatac
    20041 cgcgggcctg gaataccaaa ggatgtgctc ctgcccacgc accagcgcgg gtatgcttaa
    20101 caaaaaaatg aaaaaggaat aagggtttgg catggatctc atttcaattt ggggttgtgc
    20161 cgatgccgct ccgcatcccg gattttttta tgttccatat tcacggcgat ggcatgctgg
    20221 aggtcgacgc cgcattgatt ggccaggcag gtcaggacaa acaatacatc ggccatttca
    20281 gcggcgaggg attgggcggc tttgtccgct tccccttcgg tcctgaacga ctggtcgccg
    20341 tatattctcg ccatcagccg ggccacttct ccacattctt cagccagtat ggccgtattg
    20401 gtgagttcgc tgtaatacct cactccatga tttttaatcc attggtcgac ttcatgctgg
    20461 agctccgcga tggtcatgtt cgatataaat ttttgttcag aataaaccta cataatattt
    20521 gtgaagcgaa tagacatttt cttaaagacg aatcccaaga gcggaagaat ggaatgggcc
    20581 gattgaattc atgcgtcgaa ccttaagctg acaaaaaata tcaatacatt cgcctgccgg
    20641 gaaccatccg atatgaactg gatcgccata ttgagtgtcg cagtgggggc cgccgcgggt
    20701 ggcgtcctca ggcatctcct gtccgtctgg atgcgtgcgg atatggcccg ctttccctgg
    20761 cataccctga tcatcaatct ctccggcagt ttattcctgg gcatcctttg ggcttatttt
    20821 ttaaaacatc cgggcaatct cgcgctccgt ttggcgctca ctaccggttt ttgcgggggt
    20881 ttcaccacct tctccacttt ttccctggaa atgatcaacc tttttcagaa agggcaatat
    20941 tggtccggcg ggatctacct gttttcaagt gtcctgggcg gggttctttt ggccggcctg
    21001 gggtttgcga tgatggggaa aagttgagga gaataagtag acaggaggaa tggagacagg
    21061 aggaatggac aaccctcaat cctcaatcct caaccctcaa tcctcaatcc tcaacccttc
    21121 ttcttaatcc cccgtaacca cccagtccaa aaaaaccggc atcatttttt tccaggttgg
    21181 aacgtcgtgg cggccgcctt cgacgatgtg gaatttcatg tcgtgttcac ggtggtagcc
    21241 cttgccatgc agttcgttga tcaggtcgag ggtgtcgtcg atcacgtcga tgatcccgtt
    21301 gttgttgcgg tctgcttttt catcttcggt ccccgcctgg aaccaaaatt tgagttccgg
    21361 acgggaggag gtattcctga cgcggtgatg catcatccgg tggtgcacgt catccttgat
    21421 cttttcagga tgcctccacc agaaagaacc ggaaaaaaca ccggttttcc ggaagcggtg
    21481 cggggaatgc caggcaatgt ccatggcgct cagaccgccc aggctccatc cggcaatggt
    21541 ggtatcggta tggtggtcga gtgaatattc ctgatgaaga aagggaatca attcggagag
    21601 aataaactgg ttgtaggcgg cagccaggtg gcctcttccg gcataatcgg aggaccactg
    21661 aaccccgtat tcctgtttgc gcgcttcacc gggagcggct gcgcatccca ccaccaggat
    21721 ggaccgccgg tgatggaccg ctcgtgcctc caccatttga cccatgtcca tttgggccag
    21781 ttcctgccca tcgttgacga tcaccaggtg gtcgattttg ggccggtccg gagccggtcc
    21841 gtggtaaaaa gaaagggcga cctgcctctc cagatggtca gatcggaaat cgcggtacct
    21901 gcggttcaag gaataaaata tttatctatt ttgggtcaaa atacaaactt atgaagaaga
    21961 tcggaatcct tcacggcatg gaaagcagtt ttccacccgc ttttgtcgac cggatcaatg
    22021 ccatggatgt gccggggatc cgagccgaag ccgtcaaaat agaccgggtc atccagggcg
    22081 aaccgctcca atacgatgtc atcgtagatc gtatttccca ggatgtgccc ttttaccggg
    22141 ccatgctgaa aaacgcggcc atcaccggca ccgcggtgat taataatccc ttctggtgga
    22201 gcgcggacga taaatttttt aataattgcc tcgcggtaaa aatcggggtc ccggtaccta
    22261 aaacggtgat ccttccgtcc aaggaaaggc cgccggacac ccaggaaact tcgttcagga
    22321 acctggcttt cccgctggac tggcaaggca tgtttgatta catcggatgg ccggcataca
    22381 tgaagccttt tgccgggggc ggatggaaac atgtttataa aatccatgac caggccgatt
    22441 tttttgacaa atacgggcag accaaccaac tggtaatgct cctccaggaa gaaatcgtgt
    22501 ttgaccatta ttttcgctgt tattgcatcg gaagcaaata tgtccggatc atggcttatg
    22561 aaccccgcaa cccccatcac ctgcgttatg atgtcaggct cccggtaaat tcagaaaaac
    22621 tgatctccct tattgaatcc tatgtcatcc gcctgaacga attcctggga tacgacttca
    22681 acaccgtgga gtttgccgtg cgcgacggca ttccctatgc cattgatttt tgcaacccgg
    22741 ccccggatgc cgatctgcat tcagtgggca aagacaactg ggattgggtg gtagaacatg
    22801 ccgcccttta cgccatcgaa agggcccgtg tgcataaacc gggtcaggac aacctaacct
    22861 gggggaaata catttcccgg agtgtaaaca aagaaatgtt ataaaaatgt ggaggatcct
    22921 tctttggtcc attagtctta ttccggcgtt ttattcttac gggaccgccc aaattccgcc
    22981 gaaggactgg gtaaaattat tcaacggcaa aaacctggag ggctggaatg taaaaatcca
    23041 ccatcacgac tacggggtaa attttggcaa taccttccgg gtcaagggca gaaagatcgt
    23101 agtggcttac gatcaatacg gggacttcaa caatcagttt gggcatttat actatcataa
    23161 accctattct cattaccgga tcaaactgga ataccggttc aggggaagcc ttcaacccgg
    23221 tgcgccggac tataccctgc tcaacagcgg ggtcatgttt cattcccagg atccggccac
    23281 catgcccagg gaacaggatt ggccgatttc cgtggaaatg cagttcctgg ctggcctcgg
    23341 tgatggaaaa ccacggccca cgggcaatat gtgttccccg ggtacggata ttgtttacca
    23401 aggtaaaaaa taccctggcc attgtctcaa ttccagcagc gaaacctacg acaaagagaa
    23461 atgggtggaa gccgaactgg aggtattggg cgacagcctg gtccgtcacc ggattgaggg
    23521 caaggtggtg ctggaatata cccatcccac tatgggtggc ggagtggcca accgctttga
    23581 cccggcggtc tgggaacccg gcaaagccct gacctcgggc tttattgccc tgcaaagtga
    23641 aggacaaccc attgaattca ggaaaatcca actggtcgac ctcaagggct gcaaggaccc
    23701 cggcgccaaa aatttcaggt cctacctgga ggcccatgac ccttcaagct gtatttatta
    23761 agaaaaaaaa gctatataaa tagctgaata tcattgattt aatattatat ttaatttttt
    23821 ttatctttac ctcccgcaac caatcctgat cagggcatgt acatcaaaga gagagagtcg
    23881 attcgaatcg ccatcgtgga tctttataac ggggaggcca acgaaggaat ccgttgtatt
    23941 cacgagttga tccacagttt ttcccttgaa atcaacctgg aggtcatcac cgatacttat
    24001 gccctgcgac tggaagagga tgtacccgac ctctcctacg atatttatat cagcagcggc
    24061 ggcccgggaa gcccattgga caccaaggga atggcctggg acaccaagta ttttgaactg
    24121 attgattcca tcgaagcctg gaacaggttg cctcacgcca ggaaaaagta tgtgtttttc
    24181 atctgccatt cttttcaaat gatctgcagg cattatggcc ttgccgaaat caacgaaagg
    24241 caaccctcct cctatggcat ttttcccgtc aaactgacag ccgccggaag agctgaggcc
    24301 ttgtttgaaa ccctgccgca tcctttttat atcgcggact tcaggatgtt ccaggtgatc
    24361 cggcccgata tgaataaatt tctcagccac ggatatgaaa tcctggctga agaaaatcag
    24421 cctgctccag gcacggaaga ccgggcattg atggctatcc gtttttccga ttattttgtg
    24481 gggacccagt ttcacccgga agcggatccg gaaggcatgc tggtgcactt caacggggcc
    24541 aaaaaagccc ccattatcca taatgtgggc gaaacggcct atcacgaaat ggtcgtccac
    24601 ctaaaagatc cggacaaaat cgccaggacc agggctacca tcctgcccaa tttcctgagg
    24661 atggcggcta cccagcttga gttggtccga taggaatagg tgagtggtgg ttggtgggtg
    24721 gtggttaacc tcaacttgct aacttgctaa ctttctaact ttctaactac aatcccgtct
    24781 tcaacccgat ctccaaagac aaggacctga actgatccca gtttggacca aattgtctgt
    24841 tgaggaaacg ctgggcataa gtcagttggg aatagacatc caggctggaa ttgactttcc
    24901 actccaggcc cagggagccc tgtccgtaaa gcagggcacg cctcctgtcg aggtgggtaa
    24961 actggtcctg tacccggctg taaattgaat tttggacggc attcaatttg cgttccccgt
    25021 tgaaattgac ctgcttgatt tcaggcagta catcataaat cgccttggca ataaagttgg
    25081 cgttgatgcc ggccatggca tagggtttca ggcgggtgtt gcttttcgtg gtatacctga
    25141 cagcgattgg gagtgcaaca atatcgtatt caattttttt gaaatttata ttccaataag
    25201 gatcaaggta ataagccctg gaaggagaat aagaccgatt agcataaccc aggccgaatt
    25261 caaggccgaa ccggtttttg aggtaatgca ttttaaaccc ggcgccataa cctgtggaac
    25321 ttaccgtcgt agccggatat aaatcggatt ttttaccgaa aaccaggaac tgctggtcag
    25381 gcaggtagat ttgattgtaa tcaaaatggg aatacatacc cagtttccat ccccccttga
    25441 ccgttaattt caccacatcg ggcaactcaa ggcttgaagg ttccggtacg gggaccagtt
    25501 ggtgtgcgag caatgcttct tccccatcct ccaccggttc tccgcttaat tcctggagaa
    25561 ggggcagaga cttgaccttg ccatccagcc tggaataaat ctggccggaa ccaataagac
    25621 tttgacggga gctaattctt tccagcagta cgtcatgcct tgcaactacc ggccgggacg
    25681 ctgaggtcag gtccatcccg ccggatgtgg tggccgacag ggctccaggg gaattcgtac
    25741 cagttgtatt ccctgatgag ttatttaacg caatagaagt attattctcc gttttcggat
    25801 aaagcgtcgc aatggcctga tccccgtcat ggtttgaatt attccgtagt gccgtttcag
    25861 gttcggccgt ttttataaat ggaggggtga attttccgga acggctgatc tggaagagag
    25921 taaggaccaa caggcaaaac aggacggcct caatcatctt cgcccggtaa taatgccgaa
    25981 ggtatttttc ttccgcttcg atcctggcgg ccagtttagg ccaggtggag gcatcgtatt
    26041 tccgggtgat gcctttcagg gaattggaaa cggttttgtc aaagcgggca tgaacgaccg
    26101 gttgatccgg cacctcttcg tccagtttgc gggccataag gtcccagtgt ccggcattat
    26161 agtctgcctt aaattcaccc agagcctcat tgaccacacg gtccgtcggg tgatcttcaa
    26221 atatcttttc ctcaccctta tcgatggcgg acagcataaa caaatcccag tccctggggc
    26281 cttcggaggc ggccggctgg gttaccttgt ttctgataat atcatcaaac tcctcagtgt
    26341 tcatgcaatt ttccttttcc caacagtatt tctttcagtt taatcctggc tttgaccagg
    26401 ttggatcttg aagtgctttc cgtaatgccc agttgctccg caatttcccg gtgcgaatag
    26461 ccttccagga cataaagatt gaagaccgac cggtaggcga aagtaagttc ctggatggcc
    26521 gtaaggattt ctttttccgt gcactgactg attgcatcca catcatcggt gcctatggca
    26581 taagccgtct ccaggttttc ggtccggcga cggttttctt tccggtaata atcgatggag
    26641 gtattgacca tgatcctccg gatccaggcg gacaaagagg tgcccggctg gtacttgctg
    26701 atgtgcttaa agacctttat gaaaccttcg tgcatgatgt ccagcgcgtc ttcgtcgttg
    26761 ttggcataac gcagacagag acccatcatg atgccataat ggtcctcata gagttttttc
    26821 tgggcccatc gttcgttgcg cgagcaggct ttgatgaact cttcttcgga ttgtaaagga
    26881 atgataa
//
LOCUS       DAOQXT010000065        26883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2109_length_26883_cov_7.685702, whole genome shotgun sequence.
ACCESSION   DAOQXT010000065 DAOQXT010000000
VERSION     DAOQXT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 26883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26883
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_26883_cov_7.685702"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKM27_11965"
     CDS             <1..318
                     /locus_tag="PKM27_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08025.1"
                     /translation="SGESYYRLKLTGTDQSADYSDIVRVRVPDDENSLAFYRASTREI
                     LIHPGSVSENEVLSLFAVHGNLIIEVPGGVKSIGAESLNAGVYILVVKTENGRKVQKL
                     MVY"
     gene            complement(385..849)
                     /locus_tag="PKM27_11970"
     CDS             complement(385..849)
                     /locus_tag="PKM27_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNR08026.1"
                     /translation="MKPVLTNREGLELLRQYCNYQERCHQEVRSKGLKLGFRGDDLEE
                     LISAMITEGLLDEERYARSLARGKFRNNQWGRVKIIQALKMHNIPEYCIRKALTEIDE
                     SEYRQTLLKLAELKNESLPGGLSDYEKKQKVISYLMQKGYEFEAIESALGQE"
     gene            complement(859..1497)
                     /locus_tag="PKM27_11975"
     CDS             complement(859..1497)
                     /locus_tag="PKM27_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNR08027.1"
                     /translation="MSSIKNILFDLGNVIIDIDIPRTRQELVKLLRRPDLEGQVMETL
                     KPVLLDYEKGLLSDELFINALLRHARPQVYAQQVIRAWNAMLIDIPAERLDFLKQLRD
                     QQYRLFVLSNTNGLHLSWVNAFMEKNYQAPSLEPWFDRLYYSHLVKARKPETASFEYV
                     LRDAGIRPEETLYIEDTYENIESAQTLGIRTLYLTGGHDVITALKQYLSIKP"
     gene            complement(1567..2205)
                     /locus_tag="PKM27_11980"
     CDS             complement(1567..2205)
                     /locus_tag="PKM27_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR08028.1"
                     /translation="MTHALSSECKILIVEDEPLIAEHIATYLNNADFMVSGIAYDVDE
                     ARQLLRTSSPDAVILDINLDGETDGIQLAEFINKNYQLPFLFLTSYADRDTLERAKKV
                     EPWGYIVKPFNEKTLQATLEIAISNFAQRANQLAPGIQLEKLNRHLSTPITPREFEVL
                     QLIYEGQTNRQIAGTLFVSTNTVKRHINNAYLALEATSRSTAIARLRELMLK"
     gene            complement(2217..4220)
                     /locus_tag="PKM27_11985"
     CDS             complement(2217..4220)
                     /locus_tag="PKM27_11985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNR08029.1"
                     /translation="MKSILFLFFASLCFNRSPAQKDLISAAYWKEHKKVLISLDVAQD
                     QEKDSVLSILNNTLNHLPAQAEPLHRADLNHALGALYLDKKEFAPALRHLNAARSFFN
                     QVTPDSIDSRIISDLAKAFFHRGNYYVAGQNFMDALRTNPKPGPALENEIRDYLARIY
                     NLNASINLDPSFFIQSMELKKKLGDSLGMLRVAQRLSSTFYSEKQYARSHAFAGQAMD
                     LASRLGLPEDRIFSAIEKANCAIRLSGFPEAMALLEKIQIQIPSSDLNLLSRFETTLG
                     NYYLVQGLDSMAEIHYKKALPVRNSPLLEQNINLNKAESYEQAGRIKEALEHYRLYTR
                     QLLVGSALNVQSAITRIEENSARLKFKDEIRYLNAENRLMDSLLNKEKLLADALFSRN
                     QIQSQQLEDQTRLSASLQREMVLQEQKIADEKKMRFLFFAASLIFLLFGCLLYFMFIQ
                     QRNKNRIIEKQADEMEVLMKEIHHRVKNNLQIISSLLDLQSLSIRDQSAYTAIREGKN
                     RVQSMALIHQNLYRDGNIRSINVKDYIQHLASSLFDSYHIEKNKINLDTDIHPLLLDV
                     DTVVPIGLIVNELITNSLKYAFNPLENGDLSITLKPDGNGSLYFRVKDSGVGFPPGWS
                     PDAGNSFGFQLIRAFAKKLKAKVETYNEQGAVVALHITKYKLV"
     gene            complement(4330..5862)
                     /gene="dnaB"
                     /locus_tag="PKM27_11990"
     CDS             complement(4330..5862)
                     /gene="dnaB"
                     /locus_tag="PKM27_11990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNR08030.1"
                     /translation="MENPNPIVDIHASSARKTTRKLEDDSSIMTYGKIQPQAVPLEEA
                     ILGAIMIDKDALPVVINILKPESFYKPQNQIVYKSMLALFEKTQPIDLLTVHEALKKE
                     GELESAGGVHYLVDLSNKVGSAANIEYHARIVAQKHIQRELIRIATGTISGAFDETID
                     VFDLLDETERNLYDVTQGNLNRGYESLGALAARAQKSLEEISKREEGLTGVPSGFTAL
                     DRVTSGWQNSDLIIVAARPGMGKTSFTLALARNASFDHKMPIAIFSLEMASQQLVTRL
                     ISLEAEIEGTKMRNGKLTDDEWAKLHRVIENMAEVPIYIDDTPGINIFELRAKCRRLK
                     MHHDIKMVIIDYLQLMSGRSDNKSGTREQEISSISRALKGLAKELNIPVIAISQLSRA
                     VETRGGAKRPMLSDLRESGAIEQDADIVTFIYRPDYYEMEGMEDNLPKGVVEIIIAKH
                     RNGPLETVQLRFIDRFAKFTELDAFDFTRLKGEMPIPGQTPFSTSITRPSRMNEDDEV
                     PF"
     gene            6177..7484
                     /locus_tag="PKM27_11995"
     CDS             6177..7484
                     /locus_tag="PKM27_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNR08031.1"
                     /translation="MYRRMIIAGAVMVLIQAWVLAQPPERLIKVRVSPDHKDWTYKPG
                     EKVFYTISVSRNEEPVADCTIHYEVGPEKMTPVKKDSMKLVNGMARIDGGSMTGPGFS
                     RCVVTTWIDGKMYRGLATAGFNPNDLQPAITYPGDFMDFWNQAKSDLAKIPLDARMTL
                     LPERCTEKTNVYHVNLQNYREGGTVRLYGILCVPKKEGKYPALLRVPGAGVRPYNGDI
                     NMAERGIITFEIGIHGIPVNMDPSVYQNLGAGPLSGYWVYNLDDRDRYYFKRVYLGCV
                     RANDFIFSLPQFDGRNLAVTGGSQGGALSVVTAGLDPRVKYLGAYYPALADLTGYLQN
                     RAGGWPHMFDKNNPGHHTEAKIRTAQYYDVVNFARQIRVPGVYTWGYNDETCPPTSMH
                     PVYNVISGPKELWLYQETGHWTYPEQTEKVNQWLFTQLTKSNQ"
     gene            7554..7994
                     /locus_tag="PKM27_12000"
     CDS             7554..7994
                     /locus_tag="PKM27_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08032.1"
                     /translation="MRLVNQDDSPFIYKLYNTPDFIRFIGDKGLSDLTKAGEYIRDSI
                     RSSYDRHGFGLYLMELKDRAIPIGLCGILKRDEKNAPDIGFALLPEFYRQGYTLEAAE
                     SVLRYARERLNLQVIRGLVHPENTASRALLKKIGIPEEQIVLSV"
     gene            8089..>8537
                     /locus_tag="PKM27_12005"
     CDS             8089..>8537
                     /locus_tag="PKM27_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR08033.1"
                     /translation="MKNFMNKPTVLVETGGIKTPIRNYMATNLITFTPDSNVMDVVNA
                     LLEKRITGAPVLNDRKEVVGMIDDKTCLKLLVASEYYNSPLSEQTVSNYMDDVLTTVK
                     EDISISEAANIFLETKYKRLIVVDENGKLAGQISRRDILRAIRDINT"
     assembly_gap    8538..8637
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8796..10646
                     /locus_tag="PKM27_12010"
     CDS             8796..10646
                     /locus_tag="PKM27_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR08034.1"
                     /translation="MESNLMLYTGFLFARMRKSFHLFLILLLAGTGFSQDARLAEQYF
                     QDGEYEKAAVLFNKLLDQNKSNNYFFNRLTESLIYQDKMLEAEAIAKKQLKSFPDDAA
                     NYVVYGTLLEKKGEPKLAEEQFLKAIEKVPHDRGKITSLAGSFINMSKYDLAIEVYEK
                     GMKVFNDQNMFPYLLADMYRRKGDVPKMIQYYLISLDADPQRSVPIQSIFQRNFQPSD
                     YTELQKQLYAAIQKKPAAVHYPELLQWAMLQVKDYAGALRQARALDKMLDENGSRIFQ
                     IGQIAKSAQEWDPAIRAFEYITKEKGPQNSFYIESKKEGLACKRNKIKLSASPTSQQE
                     LMEVEQDYETFINEFGKNRNSAVLIIEYAELEALHLNNIPKAIRILDELINIPNLQRP
                     VLAQAKLNLGDYYLIQGEIWESTLLYSQVDKAFKEDQLGEEARFRNARLSYFNGDFTW
                     AQKQFDILKAATSRYISNDAIDLSVFILDNMKGDSVAEALQYFAQAELLAFQNKFEES
                     LDKLDSILYVFPEHDIHDDVLYQKAKIYRQLKQWDKAIPIYETIMEKYQEGIRCDNAI
                     FELAEIYDFQLKEKEKAKELYEKIFIDFSNSTFAVEARKRFRVLRGDKIQ"
     gene            complement(10704..10943)
                     /locus_tag="PKM27_12015"
     CDS             complement(10704..10943)
                     /locus_tag="PKM27_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08035.1"
                     /translation="MNKIKQVLGVLWMILAVVLVLFMFRQAYLKVSLATAGIMRTNTL
                     LQWIIILLVFIPICIGLFIFGRYALNREFDELEKE"
     gene            complement(11059..12102)
                     /gene="recA"
                     /locus_tag="PKM27_12020"
     CDS             complement(11059..12102)
                     /gene="recA"
                     /locus_tag="PKM27_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNR08036.1"
                     /translation="MSSERDAKLKALQLTVDKLEKTYGKGTIMKLGDRKVEDVEVIST
                     GSLGLDIALGVGGFPKGRVIEIYGPESSGKTTLAIHAIAEAQKKGGMAAFIDAEHAFD
                     RNYAESLGVDTENLFISQPDNGEQALEIAENLIRSGAIDIIVIDSVAALVPRSEIEGE
                     MGDSKMGLQARLMSQAMRKLTGAIGRTGCCCIFINQLREKIGVMFGNPETTTGGNALK
                     FYATIRLDIRRQGSPIKDKEGNMMGNTVKVKVVKNKVAPPFRVANFDIMYGEGISKTG
                     EIVDLGVDLEIIQKSGAWFSYGGSKIAQGREAAKQFMADNPEVADEIEAKIREKVAAG
                     AAIGKVAATAGDD"
     gene            complement(12253..12606)
                     /gene="ssb"
                     /locus_tag="PKM27_12025"
     CDS             complement(12253..12606)
                     /gene="ssb"
                     /locus_tag="PKM27_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNR08037.1"
                     /translation="MSNTNKVILSGYLGKEPMVKQFESGSRMATFTLATNESYLNSVG
                     KRIENTQWHKLVAWGDMAQEVERQLTKGTKIKAEGKLSHRHYEAQDGTRRYVTEIVLH
                     DFELLQFEKTQAIRA"
     gene            13002..13196
                     /locus_tag="PKM27_12030"
     CDS             13002..13196
                     /locus_tag="PKM27_12030"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNR08038.1"
                     /translation="MKDFLNYKLVLGVVLGAVAGYLYYHFIGCAQGTCMITSKPLNST
                     LYGALMGGLLVGSFKKEKQQ"
     gene            13279..16086
                     /locus_tag="PKM27_12035"
     CDS             13279..16086
                     /locus_tag="PKM27_12035"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNR08039.1"
                     /translation="MFYRSSSRSLLCILFILLFQAASGQHAVYKQLMAVDRERATTLT
                     SSTLFSRQAGGFRSDQVAKAVKKSTLVKLNRDELRQLYHSKQQRLELSIPYESGNLEL
                     ELIRTDLFSPDFTVISNESNGQALPIETGAFYRGIVRNDPNSLATFSFFPDEAIGIVA
                     TDQGNIVVGNYNTSRKEEYVIYNDHELSQPFSFECQTPEVEPGSELAEEIRRLSSQLK
                     LETRANRCVRFYLELDNTLVTEKGGAQGAVNWISGVYNQIQALYALDQISFTISQIFT
                     WTTNDPYSGSSTSTALNSFRSNRPSYNGDLAHLISRGAPTGGGVAWVNALCSTYGYAY
                     SWVNSSYSNFPTYSWTVNVLAHETGHNLGSPHTHSCTWSGGPIDGCGPTYNSAYKEGT
                     CANGPIPSGGGTIMSYCHLLSTGINLNKGFGPLPATLITNRINAATCLSASCTINVTV
                     PANVNASDGTYSDRVAVGWTGSAGNYFRVYRNTTNNSGTATALGSWQTANTYDDVSAV
                     ANQTYYYWVRAASNSSGANISAFSTSNTGWRSSVSVTTPTSVSASDGIYSDRVAVTWT
                     GSSGNYFRVYRNTTNNSGTATALGSWQTANTYDDVSAVANQTYYYWVRAASNSSGANI
                     SAFSTSNTGWRSSVSVTTPTSVSASDGIYSDRVAVTWTGSSGNYFRVYRNTTNNSGTA
                     TALGSWQTATSYNDASAATNQTYYYWVRAASNSSGANISAFSTSNSGYRSGTSGTSCN
                     PPSSLQASSITTSAFTMSWVSVSGASQYTIWYASGSSWVQLGTTSGTSVRITGMSANT
                     QYCFALRSVCGTTSSALSGSLCIRTTSSSVVSNELPSIDQEGTISFQIQALSKGNISI
                     LPNPVSLSGDFQISYYSSEESQAVIRVVDITGKTIDVVDRDLSVGENQISMEAPGVPG
                     IYSVQVQNKTGERRTLKLVVQ"
     gene            complement(16321..>16552)
                     /locus_tag="PKM27_12040"
     CDS             complement(16321..>16552)
                     /locus_tag="PKM27_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08040.1"
                     /translation="RLPKEKPGQGVTICFICEDALDLYQEFLKKELSPSEPFVGNKMW
                     VISLSDPDGYKLDFESNTEVPEGTKYKVVSGQ"
     assembly_gap    16553..16652
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16653..16895)
                     /locus_tag="PKM27_12045"
     CDS             complement(<16653..16895)
                     /locus_tag="PKM27_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08041.1"
                     /translation="MPKTNLKQAVPFFMVTDMERSLDFYVKGLGFDIKMDWQPRGRVE
                     WCWLEREGVALMLQEYREGRLPKEKPGQGVTICFICE"
     gene            complement(17140..20412)
                     /gene="ileS"
                     /locus_tag="PKM27_12050"
     CDS             complement(17140..20412)
                     /gene="ileS"
                     /locus_tag="PKM27_12050"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNR08042.1"
                     /translation="MRYNEYKSLNLPEIDKEIRAFWEKNDIFRLSVSERADGKPFVFY
                     EGPPSANGMPGIHHVMARAIKDIFCRYHTMKGEMVERKGGWDTHGLPIELGVEKELGI
                     TKEDIGKTISIADYNAACRRTVLRYKDQWDELTRRIGYWVDLEHPYITYENDYIESVW
                     NLLKILYDKNLLYKGYTIQPYSPAAGTGLSSHELNMPGTYKEVSDTTVVALFEVIRNE
                     QSAFLFDTEDEDVRIAAWTTTPWTLPSNTALTVGKNIDYVKIKTINPYTEKPQSVVMA
                     QVLAPNWIGGKHKAQDLHHSNPFKGSKLEGIRYNQLLPYGAPADGDAFRVLTGDFVTT
                     EDGTGVVHTAPSFGADDMRVARANGIGSLTLVDKQGKFTAQAGEFSGRYVKDYTDDPE
                     YKNVDIDIAVKLKQENKALNIQKYVHNYPHCWRTDKPVLYYPLDSWFIRSTAGKDRMV
                     ELNKTINWKPESTGTGRFGNWLENLQDWNLSRSRYWGIPLPVWRTEDQQEEICIGSLE
                     QLKIEINKANHALGLNQSIPDDLHRPYIDEIILVSPSGKAMKRELDLIDVWFDSGSMP
                     YAQWHYPFENQEKFRQNFPADYIAEGVDQTRGWFFTLHAIATLCFDSVAFRNVVSNGL
                     VLDKNGQKMSKRLGNAVDPFDTLDKHGADPTRWYMIANANPWENLKFDMDGLEETKRK
                     FFGTLYNTYSFFALYANIDGFYHKEAPVPLYERPEIDRWILSCLHSMIREVDLAYSGY
                     EPTQAARSIENFVDEKLSNWYVRLCRRRFWKPSLNADGSHSDDKISAYQTLYECLIAI
                     SKLMAPVAPFFAEWLYRNLNSVTRLENHESVHLALYPEFDPHKTDMDLEQRMDFAQRI
                     SSMVLSLRKREKLRVRQPLGRILIPVLDEEFIGQIDQVKDLILSEVNVKSLEYITDTS
                     GLIHKKIKANFKTLGKRLGKDMKAAAELISSLDQDAIRSLERTNHFNLDIGGNTYPIS
                     LEDVEISSEDLPGWFVTTDGKLTVALDTSLNEELLAEGIAREIVNRVQNLRKASDLQV
                     TDRIHLFLENHPDIIPAVHRFGDYIKNEVLADDIYYKDMSGMERIELTEGLEVAMEVR
                     KS"
     gene            complement(20567..20977)
                     /locus_tag="PKM27_12055"
     CDS             complement(20567..20977)
                     /locus_tag="PKM27_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNR08043.1"
                     /translation="MKHEELTSLIIGICISIHEKLGPGLLESVYEELICYELAKRNIP
                     YVRQKGIPVIYDGVRFDIGFRADIIVDDHILLEIKSIEKIQPVHFKIALSYLKLLRLE
                     VGLLINFNVVLLKEGISRLVASNTRTYNTNQDLK"
     assembly_gap    21201..21300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21509..24658)
                     /locus_tag="PKM27_12060"
     CDS             complement(21509..24658)
                     /locus_tag="PKM27_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNR08044.1"
                     /translation="MNKFLFLALVVVLHAQVLAQNASIQPDTFFKPLKWRSIGPFRGG
                     RSVAATGVVGNFTTYYMGTTGGGVWKTEDLGITWNNISDGYFKTGSVGAITVSESDPN
                     IVYVGMGEHAVRGVMTHHGDGVYKSTDAGKTWKRMGLELTQHIARIAIHPKNPDIVYV
                     AAQGALYGPSAERGVYKSTDGGKNWSKVLYVNEKTGCAELSMDMNNPLVLYAAMWEHG
                     RLPWKVISGGEGSGLYKSTDGGEHWEKIHNGLPEEKGKMAVSVCRSNPDRVYALIESD
                     SEKEAGGLFVSTDSGKSWERVTNDHRLVQRAWYYIELFADPLDEHTVYVLSAPALKSV
                     DGGKTWEVLSGPHGDYHDMWINPSDSKNMVIADDGGAGITVNGGQTWSSQANMPTAQL
                     YRINVDNLFPYRIYAGQQDNSSVVIASRELGSSGITTSSWTASAGGESAFLAFDPDDP
                     RYVLGGSYQGTIEILDTKAKAGTNIMAAPIQYLGMDAKDLKYRYNWNAPIIWSRHEPN
                     TYYHGAQKLLKTRDRGQSWTEVSPDLTRNEKEKQGKGGGPYTNEAVGAENYGTLAYVL
                     ESPHEKGVIWTGSDDGLVYLTRDGGKNWQNVTPSGLKECLINAIEVSPHDPATVYIAT
                     TRYKFNDHAPGLYKSSDYGKNWVNISSGIPYGAFTRVVREDDQHRDLLFAGTETGIYI
                     SWNGGKNWDPFQLNLPVTPITDLRIHQGDLIAATSGRSFWILDDLELLRQYKTEPDPF
                     SIYQPAPSILVNGSSELDKSSNIFTGAHRYRGVNPSTGVVIYYHLPELKIHELLTLEI
                     RDSQGHLVRTFTSRSDSLFTPWDGGPDAEPVLPKNKGLNRFVWNMRHPTTTGVEGVYI
                     ESSFAGHKVPPGKYSIILKSGGAMVSTQAEILPNPLYEVDAATYREYHSLMVKMESEV
                     DLMHKMINTLKARRDQLEALLSKIPGEDKFKPVKSDAEQLIQKMKAWDDDMIQRKSKA
                     YDDVENFPNKFTANYLFLINQTESDLPRVNKGSLERLKELDGEWKVLKSRAREILDQE
                     IPALNKRLWGLGFGAIWGD"
     gene            24854..>26883
                     /locus_tag="PKM27_12065"
     CDS             24854..>26883
                     /locus_tag="PKM27_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HNR08045.1"
                     /translation="MQATSRFNHVHYLWDEAKANSLAGDEVALFLYRSNLLGADLRLT
                     NYGGGNTSVKIRDKDPLTGNPVEVMWIKGSGGDIGTLKKSGCAALYMDRLLALEKVYR
                     GIQYEDEMVALFNHCIFDLESKAPSIDTPLHGFLPFKHIDHLHPDAAIAIAAAEDGRR
                     ITEDLFKGEIGWAEWQRPGFDLGLKLRACLDENRAKGIQLKGIMLGSHGLFTWGETSY
                     ESYVNTLEVVETCARYLSAHYGKTRPVFGGIKITGPDKTQREKQAAALAPVLRGLCSS
                     ERPMIGHFTDDERVLEFVNSFDLDKLAPLGTSCPDHFLRTKISPLVLRLDPAEDLGDA
                     VAVKEKLLPSYEEYRKLYKAYYDQCKHPDSPAMRDPNPVVLLYPGVGMFTFAKDKTTA
                     RLASEYYINAINVMKGAEAVSSYTALPRQEAFNIEYWLLEEAKLQRLPKPKALSGKIA
                     LITGSAGGIGKAIAKKFASEGACVVLNDIHEERLAEAHGEFVSAFGKDAVRATVLNVM
                     DASSIQEAFDRACLAFGGVDIIVNNAGISLSKPLTEHTEEDWDKIYDILVKGQFLVSK
                     AGVTVLKKQAMGGDIINIVSKNAVVSGPNNAAYGSAKSAQGHLTRLLAAELGPDKIRV
                     NMVNPDAVIAGSNIWAGGWAEGRAKAYGVTVEELPAYYAKRTLLNEIILPED"
BASE COUNT         6437 a         6664 c         6680 g         6802 t
ORIGIN      
        1 agtggtgaat cctattaccg tttaaaactg acgggcacgg atcagtcagc ggactattca
       61 gacatcgtcc gggtccgggt gccggatgat gaaaattcat tggctttcta ccgggcttcc
      121 acccgagaaa tcctgatcca tcccggatcg gtttccgaaa atgaggtcct ttccctcttt
      181 gctgttcatg gcaatctgat aatagaggta cccggaggag taaagtccat tggcgcggaa
      241 agcctgaatg caggggttta tatattggtt gtaaagacgg aaaatggaag aaaggtacaa
      301 aaactgatgg tgtactgaac ccggtccgga cctttgggaa tagagccctc gtctatgatc
      361 acccccgccg taaagattga atatttattc ctgcccgagc gccgattcaa tcgcttcaaa
      421 ttcatatcct ttttgcatca gataggaaat gaccttttgt tttttttcat aatccgaaag
      481 gccgccgggc agggattcgt ttttcagctc cgccagtttc agcagggtct gccggtattc
      541 cgattcatca atctcggtaa gggctttccg gatgcagtat tcagggatat tgtgcatttt
      601 aagggcctga atgatcttga ccctgcccca ttgattgttg cggaatttgc ccctggccag
      661 ggagcgggcg taccgctctt catcgagcag gccttcggtg atcatggccg agatcagttc
      721 ctccaggtcg tctccccgga aacccagttt gagtcccttt gaacggacct cctggtggca
      781 tctttcctgg taattgcaat attgcctcaa aagctcaagg ccttcccggt tggtcagaac
      841 gggtttcatc cggatcgttc agggtttgat ggacaaatat tgtttgagtg ccgtgatcac
      901 gtcatggccg ccggtaaggt aaagggtgcg gatgcccagg gtttgggcgg attcgatatt
      961 ttcataggta tcttcgatgt aaagcgtctc ttcaggccgg atcccggcat cccgcagcac
     1021 atattcaaaa gaagctgttt ccggttttct ggctttgacc aggtgggaat aataaagacg
     1081 gtcaaaccag ggctccagcg atggcgcctg gtaatttttc tccataaaag cgtttaccca
     1141 ggaaaggtgc aggccgttgg tattgctcag gacaaaaagc ctgtactgct ggtcccgcag
     1201 ttgtttcaaa aaatccaggc gttcggcggg gatatcgatg agcatggcat tccaggcccg
     1261 gatcacctgt tgggcataga cctgaggcct ggcgtggcga aggagggcgt tgataaacag
     1321 ttcatccgac aaaagccctt tttcataatc cagcaatacc ggttttaatg tttccatgac
     1381 ctggccttcc aggtccggac gccgcaacag tttgaccaat tcctgccggg tccgggggat
     1441 atcgatatcg atgatcacat tcccgaggtc aaaaaggatg ttttttatgg atgacacttt
     1501 gcaaaggtaa atgggaaatg cgggaatgta caccggtggg aatacctgac aagagaggca
     1561 ccgggtttat ttgagcatga gttctctcaa gcgggcaatg gctgtgctcc tggaggtagc
     1621 ttcgagggcc agataggcgt tgttgatatg tctttttacg gtattggtgc tcacaaacag
     1681 ggtcccggcg atttgccggt tggtctgtcc ttcataaatc aattggagga cttcaaattc
     1741 acggggcgtg atcggcgtgg acagatgcct gtttaatttt tccaattgaa tacccggcgc
     1801 cagctggttg gcccgctggg caaaattaga gatcgcgatt tccagggtgg cctgcagggt
     1861 tttttcatta aaaggtttga cgatataccc ccagggttcc actttttttg ccctttccag
     1921 ggtatcccgg tcggcatagg aagtcagaaa taaaaagggc agttggtaat ttttattgat
     1981 aaactctgca agctggatgc cgtcggtctc cccgtccagg ttgatatcca ggatcaccgc
     2041 atccggagaa gaggtccgga gcagttgacg ggcctcatcc acatcatagg cgatgccgga
     2101 taccatgaag tcggcattgt tgagataggt ggcgatgtgt tcggcgatca ggggttcatc
     2161 ctccacgatc aggattttgc attcgctgct caaggcatgg gtcatgacct accggattag
     2221 acaagtttat atttggtgat gtggagggcg accacggctc cctgttcgtt ataggtttcc
     2281 accttcgcct tcagtttctt ggcaaaagcc cggatcaact ggaacccaaa agaatttccg
     2341 gcatcgggac tccatccggg cggaaaacca acaccgctgt ccttaacccg gaaatacagg
     2401 gacccgttgc catcgggctt gagggtaatg gagagatccc cgttttccaa cggattgaac
     2461 gcgtatttaa gggaattggt gatcagctca ttcacaataa gtccgatggg caccacagta
     2521 tccacatcga ggagaagggg atggatatcc gtatccagat tgattttatt tttttcgatg
     2581 tgataggaat caaaaaggct tgaagccagg tgttggatat aatcctttac attaatgctc
     2641 cggatattgc cgtcgcggta aaggttctga tggatcaggg ccatgctttg cacccggttt
     2701 ttaccttccc ggatggccgt ataggccgac tggtcgcgga tggagaggga ttgaaggtcc
     2761 agcaggctgg atatgatctg caggttgttc ttgacccggt gatggatttc tttcatcagg
     2821 acctccattt cgtcagcctg tttttcgata atcctgtttt tgttcctctg ctgaatgaac
     2881 atgaagtata aaaggcagcc gaacaataaa aagatcaggc tggcggcgaa aaacaggaac
     2941 ctcatttttt tttcatcggc gattttttgt tcctgcagca ccatttcccg ctgcagggag
     3001 gcgctcagtc tggtctggtc ttccagttgc tgcgattgga tctggtttct ggaaaacagg
     3061 gcgtctgcca aaagtttttc tttattcagc aaagagtcca tcaaccggtt ttccgcattg
     3121 agataacgga tttcgtcttt gaatttaagg cgtgcggaat tttcttcgat ccgggtgatg
     3181 gccgattgca cattgagggc actgcccacc aaaagttgcc gggtatacaa gcggtaatgt
     3241 tccagggcct cttttattct tccggcctgt tcataggatt cggctttgtt caggtttata
     3301 ttctgctcca ggaggggaga attcctgacc ggcaaggctt ttttataatg tatctccgcc
     3361 atcgaatcca aaccctggac caggtaataa tttcccaggg tggtttcaaa gcggctgagc
     3421 aagttgaggt cagaggaggg gatttgtatt tgaatttttt cgaggagggc cattgcttcc
     3481 gggaacccgg acaagcggat cgcacagttg gccttttcaa tggccgaaaa gatccggtct
     3541 tcgggaagtc ccagccggct cgccagatcc atggcctgtc ctgcaaaagc atggctccgg
     3601 gcgtattgtt tttcactgta gaaagtactg gacagccgct gggccaccct cagcataccc
     3661 agggaatcgc ccagtttttt cttcagctcc atggattgta taaaaaagga aggatccaga
     3721 ttaatgctgg cgttcaggtt gtaaattctg gccaggtaat cccggatctc attttccagg
     3781 gcgggaccag gtttagggtt cgtgcgcaaa gcatccataa aattctggcc cgccacataa
     3841 tagttgccgc ggtgaaaaaa agctttcgcc aggtcggaga ttattcttga atcgatggaa
     3901 tccggcgtca cctggttgaa gaaggaacgg gctgcgttaa gatggcgcaa ggcaggggca
     3961 aattcctttt tatccaggta taaagctccg agggcatggt tcagatcggc ccggtgcagg
     4021 ggctccgcct gggcgggcag atgattcagg gtattgttta agatggacag gacgctgtct
     4081 ttttcctggt cctgggcgac atccaaagag atcaacactt ttttatgctc cttccaatac
     4141 gctgccgaaa tcaagtcctt ttgtgccggg ctccggttga agcacaagga ggcaaaaaat
     4201 aagaaaagga tcgatttcat tttaacggct caaaaaggtt gaagcggcaa taaagatggg
     4261 gggataaagc ggaatcccga tcacccaaac aggtgaattt ctatcaacgg aagactggcc
     4321 gccggaaggt caaaatggga cctcatcgtc ttcattcatc ctgctgggcc gggtaatcga
     4381 cgtgctgaag ggggtttggc cgggtatggg catttcccct ttaagcctgg taaaatcaaa
     4441 ggcatccagt tccgtgaatt tggcaaaacg gtcaataaac ctgagctgga ccgtttccag
     4501 gggcccgttc cggtgtttgg caataatgat ctccactacg cctttgggca ggttgtcctc
     4561 catgccttcc atttcataat aatcgggacg gtatatgaag gtgacgatat cggcatcctg
     4621 ctcgatggcg cccgattccc gcaagtcgga gagcatgggc cttttggcgc cgccccgggt
     4681 ttccaccgcc cggctgagct gggaaatggc gatgaccggg atattcagtt cttttgccag
     4741 ccctttaagc gccctggaaa tggaggaaat ttcctgctca cgcgtccccg atttattgtc
     4801 cgagcgtccg ctcatgagct ggagataatc gatgatcacc atcttaatgt cgtggtgcat
     4861 cttgagccgc ctgcatttgg cgcgcaactc gaaaatattg atcccgggcg tgtcgtcaat
     4921 ataaatgggc acttcggcca tgttttcaat gacccggtgc aattttgccc attcgtcgtc
     4981 ggtcagtttg ccgtttcgca tcttggtccc ttcgatctcg gcttccaggg agatcaacct
     5041 ggtcaccagt tgctggctgg ccatctccag ggaaaatatg gcgatcggca ttttgtggtc
     5101 aaaagaggca tttcgtgcca gggcaagggt aaaactggtt ttccccatac ctggcctggc
     5161 ggctacaatg atgagatcgg agttttgcca gccggaagtc acccggtcca gggccgtaaa
     5221 gcccgagggc accccggtaa ggccttcttc gcgtttggat atttcttcca gggatttttg
     5281 tgcccgggcg gccagggccc ccagggattc ataaccgcgg ttgaggttgc cctgggtgac
     5341 gtcgtaaaga ttcctttcgg tttcatccag aagatcaaat acatctatgg tttcgtcgaa
     5401 tgctccgctg atcgtccccg tggcaatgcg gatcagttcg cgctgaatat gtttttgagc
     5461 cacgatgcgg gcatgatatt cgatattggc ggccgagccc accttattgg acaggtctac
     5521 caggtaatgg acaccgcctg ccgattcgag ttcgccttct tttttaagtg cctcatgcac
     5581 ggtaagcagg tcgataggct gtgttttttc aaacaaagcc agcatggatt tgtagacgat
     5641 ctggttttgc ggtttataaa aactctccgg tttcaggata ttgatgacta caggcagggc
     5701 gtccttgtcg atcatgatgg cccccaaaat ggcttcttcc agcggcacgg cctgtggctg
     5761 gattttgcca taggtcatga tgcttgaatc gtcctccagt ttacgggtgg ttttccgggc
     5821 ggaggaggcg tgaatatcaa ctatcggatt tgggttttcc attcttcacc ggcaaacata
     5881 atcttaatta aaatatataa atcaatttta atgtcttgcc gctgttcaaa tgtaagacag
     5941 cgccttccaa ttgaaaaaca gcgaaccggt cctcttcacc aggccaatgt ggataagttg
     6001 ttggcagact gtggataagt gatccaaagg agcttgaggc ggtcaaaccc gtctgatttt
     6061 caataaaata actattgagc aagggtaata aaaaatgtta ataaaaaatt ctaaccagtc
     6121 aaaatccctt agtgaatctg gccggtttgt gtatcttaac catcaaatta aacagtatgt
     6181 acaggagaat gatcattgcc ggggcagtta tggttttgat ccaggcctgg gtgctggccc
     6241 agcccccgga aagactgatc aaggtaaggg tatccccgga ccacaaagac tggacctata
     6301 aaccgggtga aaaggttttt tacaccatca gtgtcagccg caatgaagag ccggtggctg
     6361 actgcaccat tcattatgaa gtcgggcccg aaaaaatgac cccggtgaaa aaagattcca
     6421 tgaaactggt taatgggatg gcccgcatcg acgggggcag tatgaccggg ccgggttttt
     6481 cgagatgtgt cgtcaccacc tggatcgatg gaaaaatgta ccggggactg gccaccgccg
     6541 gttttaatcc caatgatttg caacccgcaa ttacttatcc cggtgatttt atggatttct
     6601 ggaaccaggc caagtcggac cttgccaaaa tccccctgga tgcaagaatg acccttttgc
     6661 ctgaacgatg tacggaaaaa accaatgtct atcatgtgaa tctccagaat taccgggagg
     6721 gaggcaccgt tcgattgtat ggcatactct gtgtaccaaa aaaagaaggc aaatatcctg
     6781 ctttgctgag ggtgcccggg gcaggggttc ggccctataa cggggatatt aacatggcgg
     6841 aaagagggat catcactttt gagatcggca ttcacggcat tcccgtcaat atggatcctt
     6901 cggtatacca gaatttaggt gccggccccc tttccggata ctgggtgtac aacctggatg
     6961 accgggaccg ctattatttc aaacgggtct acctgggctg cgttcgcgcc aatgatttta
     7021 ttttcagcct tccccagttt gatggccgca acctggccgt gaccggggga agtcagggtg
     7081 gggctttgtc ggtggtgacc gcagggcttg atccaagggt gaaatatctc ggtgcttact
     7141 atccggcctt ggcggatctg acgggttatt tgcaaaaccg ggccggaggc tggccgcata
     7201 tgtttgacaa aaacaatccg ggtcatcaca cggaagcgaa aatccgcact gcacaatatt
     7261 acgatgtggt caatttcgcc cgccaaatcc gcgtccccgg ggtttatacc tggggataca
     7321 acgatgaaac ctgtccgccc acttccatgc acccggtata caatgttatt tcagggccta
     7381 aagaactgtg gttataccag gaaaccggcc attggaccta cccggaacaa acggaaaaag
     7441 taaatcaatg gttgtttacc cagttaacga aatccaacca gtgagcaaac cggttttgtt
     7501 tttgtaaaaa ttttttttca ctgaacttcc gactggaaac cgaaaggctg gtcctgcgtc
     7561 ttgtcaacca ggatgactcc ccttttattt ataaattgta caatacaccc gactttatcc
     7621 gttttatcgg ggacaagggt ttgtcggatc tgaccaaagc cggcgaatac atccgggatt
     7681 ccatccgcag cagttatgac cgacatggtt ttggtttata cctgatggaa ttgaaggatc
     7741 gggccatccc catcggcctt tgcggtatat tgaaaaggga tgaaaaaaat gctccggaca
     7801 tcgggtttgc ccttttacct gaattttaca ggcagggata tactttggaa gccgctgaaa
     7861 gcgtattaag atatgcccgg gagcgattaa acttgcaggt catccgcggc ctggtccatc
     7921 cggaaaatac cgcttccagg gccttattga aaaaaatagg aatacccgaa gaacaaattg
     7981 tactttcagt ctgaaacaaa atggaattac cgaaaaattc agattatctt gtagttagtt
     8041 ttgactatct tgagcctttg accagattaa ccgattaaaa cccgaaccat gaaaaacttt
     8101 atgaataaac ctaccgtact ggtagaaacc gggggaataa aaacaccgat ccggaactat
     8161 atggccacca atctcatcac ctttaccccg gattccaatg tcatggatgt ggtcaatgcc
     8221 ttgcttgaaa aaaggattac cggcgcgccg gttctgaacg accggaaaga agtcgtcgga
     8281 atgatcgatg acaaaacctg cctgaaattg ttggtcgcgt cggaatatta caactccccc
     8341 ctctccgaac aaacggtaag caactatatg gatgacgtgc tgactaccgt aaaggaagac
     8401 atcagcatct ctgaagccgc caatatattt ctcgaaacca aatacaaacg cctcatcgtc
     8461 gtggatgaaa acggcaaact ggccgggcaa atcagcaggc gagatatcct gagagcaatc
     8521 cgggacatca ataccatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagg
     8641 ttgaggttga ggttaaggtt aaggttaagg ttaaggttaa gattgaggtt aaggactccg
     8701 ggagaagttt ggatggatat accggtttta ttttaggatt ccttaacgga agcttgtttc
     8761 gttattgaaa agacatcatt ccaaatccca tatatttgga atcaaatttg atgttgtaca
     8821 ccggtttttt gtttgccaga atgcgtaagt ccttccattt attcctaatc ctgctccttg
     8881 ccggaaccgg gttttcccag gatgcccgtc tggcggaaca atatttccag gacggtgaat
     8941 acgaaaaagc cgcggttctt ttcaataaat tgctggatca gaacaaaagc aacaattact
     9001 tttttaaccg ccttacggaa agcctgatct accaggataa aatgcttgaa gccgaagcca
     9061 ttgccaaaaa acaattaaaa agttttccgg atgatgccgc caattatgtg gtctacggca
     9121 ccctgcttga aaaaaagggc gaaccgaaat tggcggaaga acagtttctg aaagccattg
     9181 aaaaagtgcc ccacgaccgg ggaaaaatca cttccctggc cggcagtttt atcaacatgt
     9241 caaaatatga cctggccata gaagtttacg aaaagggcat gaaagtgttc aacgaccaga
     9301 acatgttccc ttacctcctt gccgatatgt atcgccggaa gggggatgtc ccaaaaatga
     9361 tccagtacta cctgatcagc ctggatgccg atccgcagcg atccgttccc attcagtcta
     9421 ttttccagcg caatttccag ccttccgact ataccgaact gcaaaaacaa ttgtatgcag
     9481 ccattcaaaa aaagccggct gccgtgcatt accctgaatt gctgcaatgg gccatgctgc
     9541 aggtgaaaga ttacgccggt gcgctccggc aagcccgggc cctggacaag atgctggatg
     9601 aaaacgggtc gcggatattc cagataggcc agattgccaa atcggcccag gaatgggatc
     9661 cggccatccg cgcctttgaa tacatcacca aagaaaaagg cccccagaat tcattttata
     9721 tcgaatcgaa aaaagaaggc ctggcctgca aaagaaataa aatcaaactt tccgcaagtc
     9781 cgaccagcca gcaggagttg atggaggtcg aacaggatta cgaaaccttc atcaacgaat
     9841 ttggcaagaa caggaacagc gcggtcctca tcatcgaata tgccgagctg gaagccctgc
     9901 acctcaacaa tatccccaag gccatacgca tcctggacga actcatcaat atccccaacc
     9961 tccaaaggcc ggtcctggcc caggccaagc tcaacctggg cgattattac ctgatccagg
    10021 gggagatctg ggaatccact ttactgtatt cccaggtgga caaagctttt aaggaagacc
    10081 agctgggtga agaggcccgc ttcaggaatg cccgcctctc ctatttcaac ggggatttta
    10141 cctgggcgca aaaacaattc gacatcctta aggcggccac ttccagatac atcagcaacg
    10201 acgccattga tctgtcggtc tttattctgg acaatatgaa aggcgacagc gttgcggagg
    10261 ccctgcagta ttttgcccaa gccgagttgc ttgcttttca aaacaaattc gaggaatccc
    10321 tggataagct cgattccatc ctttatgtct ttccggaaca cgatatccat gacgatgtgc
    10381 tttatcagaa agcaaaaatc taccgtcaac tcaaacaatg ggacaaggcc attcccatct
    10441 atgaaaccat catggaaaaa taccaggagg gcatccgctg cgataacgcc attttcgaac
    10501 tggcggagat ctatgatttc cagctaaaag aaaaagaaaa ggccaaagaa ctttatgaaa
    10561 agatctttat tgatttttca aacagcactt ttgcggtgga ggcccggaaa cgattcaggg
    10621 tattgcgggg agataagatt cagtaggttg aagaggattg aggattgagg attgagggtt
    10681 gagggttgag ggggggctgc ccattattct ttctccaact catcaaactc cctgttcagc
    10741 gcgtaccggc cgaaaataaa caagccgata caaatcggaa taaataccag gaggataatg
    10801 atccattgca ggagggtatt ggtgcgcatg atgcccgcgg tcgccaggga aaccttcaga
    10861 taagcttgcc ggaacataaa aagtaccaac acgacagcca ggatcatcca aagaacccct
    10921 aatacctgtt ttatcttatt catcagggaa taaagatatt gcaccgggtg caataagcaa
    10981 caggaactat taagaaatta aactggaagt atatccaggc ctcccaggat ccaggatcct
    11041 tttttccaga agggaagttc aatcatcccc ggcagtggca gctaccttgc cgatagcggc
    11101 accggcggcc accttctccc tgattttagc ctcgatctca tccgcgactt cgggattgtc
    11161 ggccatgaat tgtttggccg cttccctgcc ctgtgcaatt ttggatccgc catagctgaa
    11221 ccaggcgccg cttttctgta tgatttcgag atcaaccccc aggtccacga tttctccggt
    11281 cttggatatt ccttcgccgt acatgatgtc aaaatttgca acccggaaag gaggggctac
    11341 cttattcttg accactttta ccttgaccgt attgcccatc atattgcctt ccttgtcctt
    11401 gatcggagac ccctgtctgc gtatatccag gcggatggtg gcatagaatt tcagcgcgtt
    11461 ccccccggtg gtcgtttcag gatttccaaa catgactccg atcttttcgc ggagctggtt
    11521 gatgaatatg caacagcaac cggtacgacc tatggccccc gtcagttttc gcatggcctg
    11581 ggacatcagg cgggcctgta agcccatttt gctgtccccc atttctccct caatttcact
    11641 ccggggaacc agggcggcca ccgaatcaat cacgatgata tcaatggccc cggaacggat
    11701 caggttttcg gcgatttcca gggcctgctc cccgttgtcc ggttgggaga taaagaggtt
    11761 ttccgtgtcc acgcccaggg attcggcata attgcggtca aaggcgtgtt cggcgtcgat
    11821 gaaggccgcc ataccccctt ttttctgggc ttcggcaatg gcgtgtatgg ccagggtcgt
    11881 tttaccggaa gattccggac cgtagatttc gatcacccgt cctttgggga atcctcctac
    11941 gcccagggca atgtcgagtc cgagagagcc ggtagaaatg acttccacgt cctctacttt
    12001 gcggtccccg agcttcatga tggtaccctt gccataagtt ttttccaatt tatccactgt
    12061 cagctggagg gctttcagtt ttgcatcgcg ttcgctactc attatcaatt atttataaaa
    12121 tgaaaattta aatataaact gcaaatttaa tgtaattgta aaggatttcc cgcagggaag
    12181 tgtcatggac ctggtcattt attttaagaa aatgccggca gcccgtacgt tttcctgcgg
    12241 gaaaatctgc attcatgccc gtatggcctg tgttttttcg aattgcagca actcaaaatc
    12301 gtgaagcacg atctcggtga cgtaccgcct ggtaccgtcc tgggcttcgt aatggcggtg
    12361 gctcagtttg ccttcggctt tgatcttggt ccctttggtg agctggcgct ccacctcctg
    12421 ggccatatcg ccccaggcga ccagcttatg ccattgggta ttttcgattc ttttacccac
    12481 gctgttcaga taggactcgt tggtggcgag ggtgaaggtg gccatccggg agccgctttc
    12541 aaattgtttt accattggct cttttcccag gtagccggac aggatgactt tgtttgtgtt
    12601 tgacatgatt aaatgtttta aatattaaaa aatgaatttc tttttaaccg caaccggttt
    12661 gccaaagatg tacgggcgaa agaaggacag ccggtaatta atcaattact tccgcttgaa
    12721 accgtctata aatgtttgga agcgtttgga atcgggtaaa gcatgcgaaa caatgcaatg
    12781 cgttttattc attgaaaagg gaaaggatgt ataatatgcg gaaaaatatc attgattcct
    12841 acctggtgaa tttcttacgt tccccggtta aaaaggaaca gccaaccaca atttaactaa
    12901 ttgattttct gtcaaatagg ataaaagtcc agtgtaactt ttgttaccgg tggagtcctt
    12961 ttaaactttc aattttgtat tttgcttcta taattgaatt catgaaagat tttctcaatt
    13021 ataaattggt tctcggcgtg gtcctcggtg cggttgcagg atatttgtat taccatttta
    13081 ttggttgtgc acagggcacc tgtatgatca cctccaagcc gctcaacagc accctgtatg
    13141 gagcgttgat gggaggactg ttggtaggta gttttaaaaa ggaaaaacaa caatgatttg
    13201 aagtactgga ttaattgttg ggaccattac aatatttaat ggtcttaaca gtttattaaa
    13261 gtacaaaaat cgttggatat gttttaccgt tcttcttcaa ggagcctgct atgtatttta
    13321 tttattctcc tgttccaggc cgcttcaggc cagcatgccg tttataaaca gcttatggcc
    13381 gtggaccggg agcgggccac taccttgaca agttccaccc tattctcccg gcaggccggc
    13441 gggttcaggt ctgatcaagt ggcgaaggcg gttaaaaaat ccactctggt aaaattgaac
    13501 agggatgaac tccgtcagct ctatcattcg aaacaacaga ggttggaatt gagcatcccc
    13561 tatgaaagcg gcaatcttga actggaactc attcgcaccg acttattttc tccggatttt
    13621 accgtgatca gcaatgaatc caatggccag gcccttccca tagagaccgg ggccttctac
    13681 cggggtattg tccggaatga ccctaatagc ctggctactt tcagcttttt tccggatgaa
    13741 gcaatcggca ttgttgccac ggaccagggg aatatcgtgg tcggcaacta taatacgagc
    13801 cggaaagagg agtatgtgat ctataacgac catgaactca gccaaccttt ttcatttgaa
    13861 tgtcagaccc ctgaagtcga accgggcagc gaactggctg aggaaatcag gagattgtcc
    13921 tcgcaactca agctggaaac cagggccaat cgttgtgtcc gcttctacct ggaactggac
    13981 aataccctgg ttaccgaaaa aggcggagcc cagggggcag tcaactggat cagcggggta
    14041 tacaatcaaa ttcaggcttt gtatgccctt gatcagatca gtttcactat ttctcagatc
    14101 tttacctgga ctaccaatga tccgtacagc gggtcgtcga cttccacggc cttaaacagt
    14161 ttcaggtcaa accggccaag ttataacggg gatctggccc atctgatatc ccgcggagct
    14221 ccgaccggcg ggggggtagc ttgggtaaac gccttgtgtt caacctatgg ttatgcttac
    14281 agctgggtga attcttctta cagcaatttc cccacttatt catggacggt gaatgtactg
    14341 gcccacgaga ccgggcataa cctgggttcc cctcatacgc atagttgcac ctggtcgggc
    14401 ggacccatcg acggctgcgg cccaacttat aattcggctt ataaagaagg gacttgtgcc
    14461 aatggtccaa taccctctgg tggaggaacc atcatgagtt attgtcattt actgagtacc
    14521 gggatcaacc tcaacaaagg atttggaccc ttaccggcta ccctgatcac aaacaggatc
    14581 aatgcggcca cctgcctgag cgcatcctgc accataaatg ttacggtacc cgccaatgtt
    14641 aacgcctcgg atggcaccta cagtgaccgg gtggctgtcg gttggaccgg cagtgccggc
    14701 aattatttcc gcgtgtaccg gaataccacc aacaattcgg gtactgccac cgccctgggc
    14761 tcctggcaaa ccgccaacac ttatgatgac gtttccgcag tggcgaatca aacctattat
    14821 tactgggtgc gggccgcgtc caacagcagc ggcgccaata tcagcgcttt cagcacttcc
    14881 aatacgggtt ggcggagttc cgtatccgtg accacaccaa cctccgtctc tgcatcggac
    14941 ggcatctata gtgaccgcgt agccgtgacc tggaccggaa gctcgggcaa ttatttccgc
    15001 gtgtaccgga ataccaccaa caattcgggt actgccaccg ccctgggctc ctggcaaacc
    15061 gccaacactt atgatgacgt ttccgcagtg gcgaatcaaa cctattatta ctgggtgcgg
    15121 gccgcgtcca acagcagcgg cgccaatatc agcgctttca gcacttccaa tacgggttgg
    15181 cggagttccg tatccgtgac cacaccaacc tccgtctctg catcggacgg catctatagt
    15241 gaccgcgtag ccgtgacctg gaccggaagc tcgggcaatt atttccgcgt ttaccgcaat
    15301 accaccaaca attcagggac cgctaccgcc ctgggttcct ggcaaaccgc cacctcttat
    15361 aacgatgcct cggccgccac caatcagact tattattact gggtacgggc cgcatccaac
    15421 agcagcggcg ccaatatcag cgctttcagt acttcgaatt caggttaccg cagcgggaca
    15481 agcgggacga gctgcaaccc gccttccagc ctgcaggcaa gcagcattac gacttccgca
    15541 tttaccatga gctgggtttc cgtgagcggg gcttcccaat ataccatctg gtatgcatcc
    15601 gggagttcat gggttcaatt gggcacgacc agcggaactt ccgtgcggat cacgggcatg
    15661 tccgccaata cccagtattg ttttgcgctg agatcggttt gcgggacgac cagcagcgcc
    15721 ctctcgggca gcctctgtat ccgtaccact tcgtccagcg tggtctccaa tgaactgcct
    15781 tccatagacc aggaaggcac catcagcttc cagatccagg ccttatcgaa aggaaatatt
    15841 tccattttac ccaatccggt ttccctttcc ggggatttcc agatcagcta ttattcctct
    15901 gaggaaagcc aggccgtcat ccgtgtggtg gatatcaccg gaaagacgat tgatgtggtg
    15961 gacagggatc tgtcagtggg tgaaaaccag atttccatgg aagctcccgg ggtccctgga
    16021 atttattcgg tgcaggtaca aaataagacg ggagagagga ggacattgaa gttggtggtg
    16081 cagtaagggg tggttggtga gtggtgggta gtgggtagtg gatggtgcgc ttttgcaggg
    16141 cagtttgctg aatccaaggg aaatagattt ggctttggtt ttttgaagag gctctgcatg
    16201 cttcgcgcgc tctgcggtga aatcatccgc acaaggtgat tgccaccaag cgacccgctg
    16261 gctaagccag gtgacaacca ccgctgcgct gccagactcg ctgcattcac tgaatactga
    16321 tcactgaccg ctgactactt tatattttgt tccttcggga acctccgtat tgctctcgaa
    16381 atccaattta tagccatccg gatcgcttaa ggaaatcacc cacattttat tccccacgaa
    16441 aggttctgat ggagacaatt ccttttttaa aaactcctgg tacaggtcca gcgcgtcttc
    16501 gcagataaaa cagatggtta cgccctgacc aggcttttct tttggaagcc gcnnnnnnnn
    16561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcgcaga taaaacagat ggttacgccc
    16681 tgaccaggct tttcttttgg aagccgccct tcccggtact cctgaagcat caaagccact
    16741 ccttctcttt caagccagca ccactcgacc cggccccggg gctgccaatc catttttata
    16801 tcaaaaccaa gccccttgac atagaagtcc agtgaacgtt ccatatcggt caccatgaaa
    16861 aaggggacgg cttgcttaag gtttgttttt ggcatggata taaaattaaa agcttggttg
    16921 gtttgggttc tagattttct gagtttaggc aaggaggtcg aatcatgagg cagcttaagt
    16981 tatgcgttct cggcgctctc tgcagtgact ataaagaata aaataaaccc tcctccgcac
    17041 aggtgattgc cactgggcga cccgccggct aaaccaagtg tcaaccaccg ctgcgctgct
    17101 gggcggcttc gctttcaaag acggcgaata aattcaccgc tacgatttcc tcacctccat
    17161 ggccacttcc aatccctccg tcaattcaat cctttccatt cccgacatat ccttgtaata
    17221 aatatcatcc gccagcactt cgtttttgat atagtcgccg aaccggtgaa cggcggggat
    17281 gatgtccgga tggttttcga ggaaaagatg gatgcggtcg gtgacctgaa ggtcggaggc
    17341 tttgcgtaga ttctggaccc ggttgacaat ctcgcgggcg atgccctcgg caagcaattc
    17401 ttcattgagg ctggtgtcga gcgctacggt cagtttgcca tcggtggtta caaaccatcc
    17461 gggcaggtct tcgctgctga tctccacatc ctccagactg atgggatagg tattgcctcc
    17521 gatatcaaga ttaaaatgat tggtccgttc cagggaacgg atggcgtcct ggtccagaga
    17581 ggagatcaat tcagcagcgg ctttcatgtc tttgcccagc cttttcccca gggttttgaa
    17641 attggcttta atctttttat ggatcaggcc gctggtatcg gtaatgtatt cgagggattt
    17701 gacattgact tccgaaagaa tcagatcctt gacctggtcg atctgaccga tgaactcctc
    17761 atcaagcaca gggatcagga tccttcccag cggctggcgt acccggagct tttcacgctt
    17821 gcgcagggaa aggaccatgg aactgatgcg ctgtgcaaaa tccatacgct gctcaaggtc
    17881 catatccgtt ttatgggggt caaactcagg atacaaggcg agatgaaccg attcatggtt
    17941 ttccagacgg gtgacagagt tcaggttccg gtacaaccat tcggcaaaaa acggagccac
    18001 aggggccatg agtttgctga tggcgatcaa acactcatac aaagtctgat aagccgagat
    18061 cttatcatcg ctgtggctgc cgtccgcatt gagggagggt ttccagaacc gcctgcggca
    18121 caggcgaaca taccagttgc tcagtttttc atcgacgaaa ttttcaatgc tgcgggcggc
    18181 ctgtgtgggc tcgtatccgg aataagccag gtccacttcg cggatcatgg aatgcaggca
    18241 ggacaggatc caccggtcga tctcgggacg ctcatacagg ggcaccggcg cttctttgtg
    18301 gtaaaagccg tcgatattgg catagagggc aaagaaggag taggtattgt aaagggtgcc
    18361 gaaaaactta cgtttggttt cttccaggcc atccatgtca aacttgaggt tttcccaggg
    18421 gttggcattg gcgatcatgt accagcgggt ggggtcggcc ccgtgtttat ccagggtgtc
    18481 gaatggatct acggcattgc caagccgttt ggacattttc tgtccgtttt tatccaggac
    18541 caggccattg gataccacgt tgcggaaagc cacggaatca aaacacaggg tggcgatggc
    18601 atggagtgtg aaaaaccagc cgcgggtttg gtcgacgcct tcggcgatat aatcggccgg
    18661 gaaattctgc ctgaattttt cctggttttc gaagggataa tgccattggg cataaggcat
    18721 ggacccggaa tcaaaccaaa cgtctatgag gtccagctcc cttttcatgg ctttgcccga
    18781 aggggatacc aggattatct cgtcaatata aggacggtgc agatcatccg gtatggattg
    18841 attcagtccc aatgcatggt ttgccttatt gatttcaatt ttcagttgct ccaaagagcc
    18901 aatacaaatc tcttcctgct gatcttccgt gcgccagacg ggcaggggaa tgccccagta
    18961 acggctccgg gacaggttcc aatcctggag gttttccagc caattgccga aacggccggt
    19021 gccggtgctt tcgggcttcc agttgatggt tttattcagt tccaccatcc tgtccttgcc
    19081 cgcggtggac cggataaacc aactgtccag gggataatac aaaaccggtt tgtcggtgcg
    19141 ccagcaatgc ggataattgt gcacgtattt ctggatattc agggctttgt tttcctgctt
    19201 gagtttaacg gcgatatcga tgtccacatt tttgtattcc ggatcgtcgg tatagtcctt
    19261 gacatagcgt cctgaaaatt cgcctgcctg ggctgtaaac ttgccctgtt tgtccaccag
    19321 ggtcagcgaa ccgatcccgt ttgctcgggc tacccgcata tcatcagccc cgaaggaggg
    19381 agcggtatgc actacccccg taccgtcttc cgtagtgaca aaatcaccgg tcagtacccg
    19441 gaaggcatcg ccatcggcgg gagcgccata aggcaagagc tgattatacc ttatcccttc
    19501 cagtttggac cccttaaacg gatttgaatg gtgaagatcc tgggctttgt gttttccacc
    19561 gatccagttt ggggccaata cctgggccat cactacactt tgtggtttct ccgtataggg
    19621 attgatggtt ttgattttta catagtcgat atttttcccg acggtcagtg cagtattgga
    19681 gggcaaggtc cagggcgtag tggtccaggc tgcaatgcgc acatcctcgt cctcggtatc
    19741 aaagagaaag gcggactgtt cattacggat cacttcaaaa agggcgacca cggtggtatc
    19801 gctgacttct ttatatgtac ccggcatatt gagctcgtgc gaactcaatc ccgtgcccgc
    19861 agcgggagaa taaggctgga tggtataccc tttatagagt aggtttttat cgtagaggat
    19921 tttcagcagg ttccagaccg attcgatata atcgttttca taggtgatgt aaggatgctc
    19981 gaggtccacc cagtaaccga tgcgccgggt gagttcgtcc cactggtctt tataacgcaa
    20041 gacggttctg cgacatgcgg cattatagtc cgcaatggat atggtcttgc cgatatcctc
    20101 cttagtgatg cccagttctt tttccacacc gagttcgatg ggcagtccgt gggtgtccca
    20161 tccccctttg cgctccacca tttcaccttt catggtatgg tagcggcaga aaatatcctt
    20221 gatcgcccgg gccatgacat ggtggatgcc gggcatgccg ttggcggagg gagggccctc
    20281 ataaaataca aagggcttgc cgtcagccct ctcggagaca ctcagcctga agatgtcgtt
    20341 tttttcccag aaggcccgga tttccttgtc tatctcgggg aggttcaggg acttatattc
    20401 gttataacgc ataaaacagg gggcaaagat agggagaaga tttctcacgg gggtttcgtg
    20461 tggtgagttt cacacggaga gaggcgcacg gagagcacgg ggggcgcgga ggtatttttt
    20521 attattctcc tacggtgagt gacgtattgg ggcacggagc tgttttttat ttcaagtcct
    20581 gattggtgtt gtacgttctg gtattactgg caacaagtcg tgagatcccc tcttttaaca
    20641 aaacaacgtt gaaatttata agcaaaccga cttctaatcg aagtagtttt aaataactca
    20701 aagcaatttt aaaatggacc ggctgtattt tttcaataga ttttatttcg agcaggatat
    20761 gatcatccac gatgatgtcg gcacggaatc caatgtcgaa tcttacccca tcgtaaatca
    20821 ccggaattcc tttctgtctt acataaggaa tattgcgctt ggccagttca taacaaatca
    20881 attcttcata taccgattcc agtaaaccgg gtccaagttt ttcatggatg gaaatacata
    20941 tgccaatgat caaagaagtg agctcttcgt gtttcatgaa ataaacctaa tgaaatgccc
    21001 ggtcataaac acggacaact gaggcctttt caggacgggg taaaccttaa attgtttttt
    21061 tcgtaccctt tgatgatccc tatatagaag aatgcactat ttgtaatgca caaaaaaaat
    21121 ttcccattaa ctctcagtga tcccagcgct ctccgtgcgc cacatccccg gcttccccct
    21181 ggaagccaaa attcttatga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 taagacacaa aaaaatctcc cattaatcct ccgtgccctc cgcgctctcc gtgcgccaca
    21361 tccccggctt ccccctggaa gccaaaattc ttatgacttc actctttgat cctcctcaat
    21421 tgaaatcatg catattctgt aagacacaaa aaaatctccc attaatcctc cgtgccctcc
    21481 gcgctctccg tgcgaaccct ctgtgcactc aatcccccca aatcgcccca aaccccaaac
    21541 cccacaacct cttattcaac gccggaatct cctgatccag gatctccctg gctcttgatt
    21601 tcagcacctt ccactccccg tcgagctcct tcagccgctc cagcgatccc ttattgaccc
    21661 tgggcaggtc actctccgtc tggttgatca ggaacaggta gtttgctgtg aatttgttgg
    21721 ggaagttttc gacgtcgtcg tatgcctttg atttacgctg gatcatatca tcatcccagg
    21781 ctttcatctt ttgaatcagt tgctccgcat ctgatttaac aggcttgaat ttgtcttcac
    21841 cagggatctt ggaaagcaag gcctccagtt gatcgcgtct ggccttcagg gtattgatca
    21901 tcttgtgcat gaggtccacc tccgattcca ttttaaccat aagtgaatgg tattccctat
    21961 aagtggcggc atcgacttca tacaatggat tgggcaggat ctccgcctgg gttgaaacca
    22021 tcgcacctcc cgatttgagg atgatggaat atttacccgg tggaaccttg tgccccgcaa
    22081 aactgctctc aatataaacg ccttccactc ccgtcgttgt tggatgacgc atattccaga
    22141 caaaacggtt taatcctttg tttttaggca atacgggttc cgcatccggc ccgccgtccc
    22201 agggcgtaaa gaggctatca ctccttgagg tgaaagtgcg caccagatgg ccttgggaat
    22261 cccggatctc cagggtaagc aattcatgga tctttagctc cggcaaatga taataaatca
    22321 caactccggt tgatgggttc accccccggt accggtgggc cccggtaaat atattcgaag
    22381 atttatccag ttcgctggag ccgtttacca ggatcgacgg tgcgggttga tagatcgaaa
    22441 aaggatcagg ctctgtttta tattgacgca acaactcaag atcgtccaaa atccaaaacg
    22501 acctgccgga tgtagcggcg atcaaatctc cctggtgtat cctcaaatcg gtaatgggtg
    22561 ttaccggtaa gttgagctga aaaggatccc aattcttacc gccattccaa ctgatgtata
    22621 tcccggtttc ggtgcctgca aacaacaaat cccggtgttg gtcatcctcc cggactacac
    22681 gtgtgaaggc gccgtatgga attcccgaac tgatattcac ccagttttta ccatagtctg
    22741 agcttttata cagacccgga gcatgatcgt tgaatttgta ccgcgtggtg gcgatataca
    22801 ccgtcgcggg atcatgaggg gatacctcta tcgcattaat caaacattcc ttcaagccgg
    22861 aaggcgttac gttttgccag ttcttgcctc catcccgggt caaatagacc aatccgtcat
    22921 cgctgcccgt ccagatcact cctttttcat gcgggctttc cagtacatag gccagggtgc
    22981 cgtaattttc ggcgccaaca gcttcattgg tataaggccc accccccttg ccttgttttt
    23041 ccttttcatt ccgggtgagg tccggggata cttccgtcca gctctgtccg cggtccctgg
    23101 tcttcagaag tttttgcgcc ccgtgataat aagtattggg ttcatgccgc gaccagatga
    23161 tcggggcatt ccagttgtac cggtatttta aatctttcgc gtccatgccg agatactgga
    23221 tgggcgccgc catgatattg gtacctgctt tcgctttggt gtccaggatt tcgatcgtgc
    23281 cctgataact tcctcccaaa acgtatcgcg gatcatccgg atcaaaagcg aggaaggcgc
    23341 tttcccctcc tgcggaagcg gtccagctgg aggtggtgat gccgctgctg cccaattcgc
    23401 ggctggcgat taccactgaa ctgttgtcct gctggccggc ataaatgcgg tatggaaaca
    23461 ggttgtccac attgatgcga tacaattggg ccgtgggcat attggcctgg gaagaccagg
    23521 tctggccacc gttgacggta atgcccgccc cgccatcgtc ggcaatgacc atattttttg
    23581 aatcggaggg attgatccac atgtcatggt aatctccatg cggccccgaa aggacttccc
    23641 aggttttgcc cccatccacc gatttaaggg ccggagcgct gagcacatat accgtatgtt
    23701 catccaacgg atccgcgaac aattctatat aataccaggc ccgctgaacc aggcggtggt
    23761 cattggtcac cctttcccag ctttttcctg aatcggttga aacaaacagg cctcccgcct
    23821 ctttctccga atcactttcg atcaatgcat atacccggtc cggattggaa cggcagactg
    23881 aaacggccat cttccccttt tcttccggaa ggccattgtg gattttttcc caatgctccc
    23941 ctccatccgt tgatttatat agcccgctgc cttcgcctcc gctgattacc ttccagggca
    24001 gacggccgtg ttcccacatg gcagcgtaga ggaccagggg attgttcatg tccatgctca
    24061 gttccgcgca tcctgttttt tcatttacat acaatacttt tgaccaattt ttcccgccat
    24121 cggtagattt atagaccccg cgctcggcgg atggtccata caaagctccc tgtgcggcta
    24181 catagacgat atccgggttt ttcggatgga tggcgatgcg ggcaatatgt tgggtgagtt
    24241 ccagtcccat tcttttccat gttttgccgg catcggtgga tttataaact ccgtcgccat
    24301 gatgggtcat taccccacgc accgcatgct ctcccatgcc cacataaact atattgggat
    24361 ccgattcgga aacggtgatg gctcccaccg atccggtttt aaaatagccg tccgagatat
    24421 tgttccaggt aatacccaga tcttcggtct tccagacccc gccgccggtg gtccccatat
    24481 aataggtggt aaaatttccg accacccctg tggccgcgac ggaacggcct ccccgaaaag
    24541 ggccaatgct ccgccatttc aaaggtttaa aaaaagtatc cggctggatg gatgcgtttt
    24601 gggccaagac ctgcgcatgt aataccacga ccagcgcaag gaatagaaat ttattcattt
    24661 ttggtttaat tacggcttca ggaattgccg gtgaatatag tttctttgga gcgggattga
    24721 aaggatctcc gtgcccctcg gggcccctgt gagaaatatt taatttaatg gaactcctgg
    24781 ccatagccga aagggaacgg aaactccgca tgctcacccc caggtccgca attcaattta
    24841 aatttgccct cctatgcaag ccacgagtcg ttttaatcat gtccattacc tctgggacga
    24901 agccaaagcc aattccctgg caggagatga agtcgccctt ttcctctacc ggtccaacct
    24961 gctgggcgcc gacctccggc ttacgaatta tggagggggc aacacctccg taaaaatcag
    25021 ggacaaagac cctcttaccg gaaacccggt tgaagtcatg tggatcaagg gatcgggcgg
    25081 ggatattggt acccttaaaa agtcgggttg cgccgccttg tatatggacc gcctcctggc
    25141 cctcgaaaag gtttaccggg gtatccaata cgaggacgag atggtggccc tctttaacca
    25201 ctgcatcttc gacctcgaat ccaaagcacc ctccatcgat acccccctgc atgggttttt
    25261 accttttaag catatagacc acctgcatcc cgatgcggcc attgccatcg ccgcggcgga
    25321 agacgggcgc aggataaccg aagatttgtt caaaggggaa atcggctggg cggaatggca
    25381 gcggccggga tttgaccttg ggctcaaact ccgggcctgc ctggatgaaa accgcgccaa
    25441 agggatccag ctcaaaggga tcatgctggg ctcgcacggt ttgtttacct ggggtgaaac
    25501 ttcttacgaa tcctatgtca ataccctgga agtggtggaa acctgtgccc gttacctgag
    25561 cgctcattat ggaaaaacca ggccggtatt cggtggaata aaaatcaccg gaccggataa
    25621 aacgcaacgt gaaaaacaag cggctgccct ggctcctgtt ttaaggggtc tctgttcttc
    25681 ggaacgcccg atgatcggtc attttaccga tgacgagcgg gtgcttgaat ttgtaaattc
    25741 gtttgatctc gataaactgg ctcccctggg cacttcctgc cccgatcatt ttttaaggac
    25801 caagatcagc ccgctggtgc tgcgcctgga tccggccgaa gacctgggcg atgccgtggc
    25861 ggtgaaagaa aaattactgc cttcctatga agagtaccgc aagttgtaca aagcgtatta
    25921 cgaccagtgc aaacacccgg acagccccgc catgagggat cccaaccccg tggtcctgct
    25981 ctatcccggg gtcggcatgt tcacttttgc caaggacaag acgacggccc gcctggccag
    26041 tgaatattat atcaacgcca ttaacgtgat gaaaggggct gaagccgtct ccagctatac
    26101 ggcccttccc cgccaggaag catttaatat tgaatattgg cttttggagg aagccaaact
    26161 gcagcgcctg cccaaaccca aagccctttc cggtaaaatc gccctcatca caggatcggc
    26221 gggcggaatc ggcaaggcca tcgccaaaaa atttgcaagc gagggcgcct gcgtggtatt
    26281 gaacgatatc cacgaagaaa gactggcaga agcccatggg gagtttgtat ccgcttttgg
    26341 caaggacgcc gtaagagcca cagtcctgaa tgtcatggat gcctcttcga ttcaggaggc
    26401 ctttgaccgg gcttgcctgg ccttcggggg tgtggacatc atcgtcaaca atgccggcat
    26461 cagcctctca aaaccattga ccgaacatac ggaagaagac tgggacaaga tctacgacat
    26521 tttggtgaaa gggcagttcc tggtttcgaa agccggagtg acggttttaa aaaaacaggc
    26581 gatgggcggg gatatcatca atatcgtatc caagaatgcg gtggtctcag ggcccaataa
    26641 tgcagcctac ggatccgcca aatcggcaca gggccacctg acccgcctcc ttgccgccga
    26701 attagggccg gacaagatcc gggtgaatat ggtcaatccg gatgccgtga tcgcggggtc
    26761 taacatctgg gcgggcggat gggcagaagg ccgcgccaag gcttacgggg taaccgtgga
    26821 agaattgccc gcttattatg caaaacgcac cctgcttaat gaaatcatcc tgcccgaaga
    26881 cat
//
LOCUS       DAOQXT010000066        25583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2247_length_25583_cov_6.258931, whole genome shotgun sequence.
ACCESSION   DAOQXT010000066 DAOQXT010000000
VERSION     DAOQXT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 25583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25583
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2247_length_25583_cov_6.258931"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKM27_12070"
     CDS             <1..225
                     /locus_tag="PKM27_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNR08046.1"
                     /translation="YKRKVLETLDLDAIRFIGYAFQIEMKYKSWYKGFKIKEIPITFT
                     DRVAGTSKMHGGIIKEAALGVIQLRLNRKK"
     gene            546..1025
                     /gene="mraZ"
                     /locus_tag="PKM27_12075"
     CDS             546..1025
                     /gene="mraZ"
                     /locus_tag="PKM27_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNR08047.1"
                     /translation="MSRFTGEYQSTVDDKGRVKLPAGLIRQMEEQGMVLNFVINRGFE
                     KCLMLYPKPVWDQKAAEVDKLNIYDPVQRQFVRYFYRGATQILADPADRLLLPKNLTE
                     YAGLEKNVTLFAYLNQIEIWDQKKYNAELANEPDQFSELAANVFGGVQPASSIPKGI"
     gene            1025..1933
                     /gene="rsmH"
                     /locus_tag="PKM27_12080"
     CDS             1025..1933
                     /gene="rsmH"
                     /locus_tag="PKM27_12080"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNR08048.1"
                     /translation="MEYHVPAMVQECLEGLHIQPDGIYVDATFGGGGHSRAILEKLGV
                     NGRLIAFDQDEQAFQNRIADRRFLLIHSNFRYLRRFLKYYGIGQIDGLLADLGVSSYQ
                     LDMPGRGFAFRHTGPDLPLDMRMNQQQPRTAADVINRTDPAKLEKILREYGEVWNAHR
                     LVELIMQRRERNPVTTVAQLEDLARSVAVGPVNQYLAKVFQAIRIEVNEEMTSLRQLL
                     NDAVEVLKPGGRLVILSYHSLEDRMVKHLIQTGDVDGLVKKDPFGNKIIKLRAVSKKS
                     IRPSEDEIKENYRVRSARLRIAEKSA"
     gene            2018..2329
                     /locus_tag="PKM27_12085"
     CDS             2018..2329
                     /locus_tag="PKM27_12085"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNR08049.1"
                     /translation="MEERSQNKWLQQILAGGMVIKHLPFIYFAGILALLYIANAHQAE
                     RKVRKIHKLREEVKELRWQYMSFRSEFLFSSSPTQLANDVRPLELGYHKQSSTKKLVK
                     K"
     gene            2333..4426
                     /locus_tag="PKM27_12090"
     CDS             2333..4426
                     /locus_tag="PKM27_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNR08050.1"
                     /translation="MKDEIIIRTYVVFLLFVLAGFLILGRVVQIGIVEGESWRGRGDS
                     LYVRHQPIEPERGNILSSDGRMLVTSLPLFEVRMDLLAVPKDKFVKNIDSLALCLSRH
                     LNPVISERAYRDLLWRERQAGNRYLLIKEEVTYSQLEKIKTFPLFRLGGNKGGLIVVR
                     KSKRERPYRMLAARTLGSVREGAKPVGLEGYYDDVLGGEKGSRLVQKVARDIWVPVDD
                     IGDFEPKRGKDIITHIDIDLQDITEQALYKSVAHHQALGGTAVVMEVKTGAIKAMANL
                     VRSGDDIGEYLNYAVGAATEPGSTFKLATMMALLEDGYIRLDDRVDIHGGEVELCGKK
                     IRDSEKRKGSLPTLKQVFAQSSNVGVAVTAQKYYEKNAKKFVKRLEQFGLNERSRIDL
                     EGEPAPYIKEAYNVEQKWSCTSIPWMAHGYELTLTPLQLLTFYNAVANKGRMMKPQLV
                     HQILEYSEPVKTYKPQVLKNQICSHSTLQQAMELLKSTTDSGGTAYALRNRLYKVAGK
                     TGTAVTNYYKGDINQGKKYQASFAGFFPADDPVYSCIVVIYEPSANGYYGASAAMPVF
                     REIADYSVASRFIATEDQAPVNTAPLPARIRAGGYATDIRTLLHFSGVRFENRCLNEW
                     ASLSPTESQPELTGINLQQDLVPDVRGMGLRDALFLLENKGLKVRLQGHGRVRSQSIA
                     PGSKIQGQTIELTMG"
     gene            4430..5884
                     /locus_tag="PKM27_12095"
     CDS             4430..5884
                     /locus_tag="PKM27_12095"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNR08051.1"
                     /translation="MNWTELKKHIQFFDIKGNAEFEVAGVESDSRRVKENFVFIAQKG
                     LVTDGHRYIGAAVEQGARAILCESLPEVLSPGVEFLQVKDPALAAGILADGFYGQPSS
                     RLKLVGVTGTNGKTTTATLLYRLMKALGFKAGLLSTVRNYIHDRAMETSHTTPDVIRI
                     NEMLALMVGAGCDYAFMEVSSHAMKQQRTAGLRFAGGVFTNLTHDHLDYHGTFKDYLD
                     CKKLFFDQLPRDAFAVTNKDDRNGMVMVQNTRARVVTYALKQLAEVKGKIKANTLQGL
                     VLEVNGMEVHSRLIGAFNGYNFLACFAAARELGFEPMEILAGMSGLPAVDGRFDTVYH
                     AEKKVLGIVDYAHTPDALTNVLDTIKELKAPGSRVITVVGCGGDRDKTKRPVMARIAM
                     EKSDAVIFTSDNPRTEDPQQILDDMIAGLEPQQMHRMIRQSDRREAIKSAARMADRPG
                     DIILIAGKGHETYQEIQGKRFPFDDKNILKEVLI"
     gene            6007..7260
                     /gene="mraY"
                     /locus_tag="PKM27_12100"
     CDS             6007..7260
                     /gene="mraY"
                     /locus_tag="PKM27_12100"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNR08052.1"
                     /translation="MLYHLFEYINNQVDLPGAGLFRYISFRAGGALIFSLLISMLFGQ
                     RIIKYLQAKQIGETVRDLGLHGQKEKEGTPTMGGIIIILAILLPCILWARLDNVYVLL
                     MIAVTLSLFFIGYLDDYIKVFKKNKQGLKAVTKLTGQIAVGLIVALTLLYNKNVLIRV
                     TEKEAVDNRFEILERLSASANPGNPEVLVHARSLSTNVPFTKGNAFDYAGILRWTGDA
                     ARTLTPLLYIIIIIFIITAVSNAVNITDGLDGLATGVSSIVGVVLGVFAYVSGNSIFA
                     NYLGIYFIPNSGELVIFTACLIGACIGFLWYNAYPATVFMGDTGSLTLGGIIATLAVV
                     LRKELLLPVLCGIFLIEILSVMVQVSYFKYTRRKYGEGRRIFKMAPLHHHYQKSGMHE
                     SKIVNRFWIVAVLLAVVTIITLKIR"
     gene            7262..8482
                     /locus_tag="PKM27_12105"
     CDS             7262..8482
                     /locus_tag="PKM27_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNR08053.1"
                     /translation="MLQITTKKEWKGDKVMWLIIGILIGFGLLAVYSATGSMAYLRYK
                     GNTEVYLLKQLVMAGIGLLCMYACHRQNYLIYNKLAPVLFLLAIPLLLYTLAFGVETN
                     DAKRWITLPVINLSFQTSDYARLALIMYLARSLSNKQEYIKDFKSAFIPVMVPVLLIC
                     GLIAPANLSTALLLFATSVMLMFVGRVSFKYIFMMAFFGILLFGTVVLIWWFFPDAVR
                     VDTWISRITDFISNENGGGYQVQQSKIAIANGSWLGLGPGNSTQRNFLPAAYADYIYA
                     IICEEYGLIGATGILLLYLWLLFRVSRIVMKSPKAFGAILAMGLTLNLVVQAFANMAV
                     SVNLVPVTGLTLPLVSMGGTSVLFTSVAFGIIMSVSKFIESIQEEQPAPENELMKMAN
                     TSPKPKTVEDESDH"
     gene            8466..9566
                     /gene="murG"
                     /locus_tag="PKM27_12110"
     CDS             8466..9566
                     /gene="murG"
                     /locus_tag="PKM27_12110"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNR08054.1"
                     /translation="MKVIISGGGTGGHIFPAIAIAQELKARVPGVNILFVGARGRMEM
                     EKIPQAGFEIIGLDVVGFNRSSLVKNFSLPWKLVKSLYQAWRILVKYKPGVVVGVGGY
                     ASGPVLYLANWLRIPTLIQEQNSFAGKTNMILGKKAVKICVAYDHMERFFPKEKIVVT
                     GNPVRKDLEGLESKRKEAMAYFGLDQDKKTILVMGGSLGARSINESMTAGHEQISGQK
                     SIQWIWQYGKQGAEKFGRSATAGLDNVKAMAFIDRADLAYAAADVLLCRAGALTISEV
                     LIANKPVVLVPSPSVAEDHQTHNAKALSDAGAAWLLPDREVVQNGTAQVIRLLQDQET
                     MTRMKQAQKSLARPQAAAAIVEQILHLNPVAA"
     gene            9563..10357
                     /locus_tag="PKM27_12115"
     CDS             9563..10357
                     /locus_tag="PKM27_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08055.1"
                     /translation="MKKFLRNIGTDQNMVRLGMGLTVLLFLMFYERAGRFKNRALSGQ
                     MQVHVQHLENGNDFITTEEMRSKILRKFQQEFSNVPLATLDLQEIEAGLQSMDFIKST
                     DVYLDASNRLNIFVKQREPILRILTPEGNSLYVDKDGSIMPASPHYSPRVLVAFAPVP
                     MTKDSLELSKPGMEKDLFELANFIARDRFLHALVEEIMVHSQNDWTLIPKFGPSRIFL
                     GDLERLEDKTDKLKRVYREILPGQGWDLYYSVNLKFKGQVICTKRA"
     gene            10391..11764
                     /gene="ftsA"
                     /locus_tag="PKM27_12120"
     CDS             10391..11764
                     /gene="ftsA"
                     /locus_tag="PKM27_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNR08056.1"
                     /translation="MNARDFNKKNVVAALDIGTTKVCCIVGRKDEYGKIDVLGASKVD
                     SEGVLRGVIANIDKTVKAISDAVDLAEKDAQCEIQEVYVGIAGQHIKSLQHRGILARD
                     NNEKEISRADIDRLVQDMYKLGLTPGDKILHVIPQEFTVDNEQGIVDPIGMSGHRLEA
                     NFHIITGQIAATNNIMRCVERANLTVADMTLEPIASAEAVLSKEEKEAGVVMVDIGGG
                     TTDITIFQEGIIRHTAVVAFGGNIITRDIKEGCTVMTEQAEKLKIKFGSALADEIVDN
                     RIITIPGLKGRDPKEISEKNLARIIQARLEEILDLILYEIRRSGYEKKLIAGIVLTGG
                     GALLKNFELLCEYHTGLQTRIGYPNEHFGYGFKDNISSPIYATGVGLLMYGLKMKESQ
                     QRMLLQTRKDRPAERPEPVTQPELGLPGVPAPKTASPIPAQGKQKKPLVDFIKNFFEP
                     SPDPDLR"
     gene            11818..13239
                     /gene="ftsZ"
                     /locus_tag="PKM27_12125"
     CDS             11818..13239
                     /gene="ftsZ"
                     /locus_tag="PKM27_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNR08057.1"
                     /translation="MKFEIPKESGNIIKVIGVGGGGSNAVSHMYRQGIVGVDFIICNT
                     DNQAMETSPVPVKIQLGPQLTAGRGAGSKPHMGKQACIESLEEIQALINENTKMVFIT
                     AGMGGGTGTGAAPIVAKIARELDILTVGIVTVPFPFEGKRRKEQAIEGIEEMKRNVDA
                     LIIISNEKLRQIYGNLPVSEAFGNADNILSTAAKGIAEIITMPGYVNVDFEDVNTVMR
                     NSGVAIMGTASASGERRAYRAVVDALSSPLLEENDIRGAQHILLNISYGTQEVTMDEI
                     YEITEYVQEEAGYGTDIIWGNCFDERLEDQICVTVIATGFEQNFGHKPVEVREKIKVS
                     LEEEKPTTSLHEVFDIGDQTNDKTIEFEPAYREPMIKNQEEPFIRSESGAENSGRHEE
                     EKRARPGGSETRSETGRKMRFQMHQMRNTSEMENQPAYLRRNIPLEDVPPSKDHPSPK
                     WTLSSDDEPQIRENNSYLYDNVD"
     gene            complement(13471..14280)
                     /locus_tag="PKM27_12130"
     CDS             complement(13471..14280)
                     /locus_tag="PKM27_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNR08058.1"
                     /translation="MKPTQKYLFETIFEADTPAGKKFDVVLMVLIVVSVILANLETLA
                     NLPPSIQNLFFILEWGFTVIFTIEFILRLIVAPKPLKYILSPYGIIDLIAIVPTYLSL
                     ILTGTHAIIIVRALRLLRVFRLFEMSGYVNQGAILITAFKRTKDKIIIFLFFIFIVVN
                     IVGALIYMLEHPANPNFDSLPRSVYWAIVTLTGLGYGDIVPKSAFGQILASFVVLLGY
                     AIIAVPAGLITKEYARTDKSIHFSNQVCPHCFKEGHQTDAVYCRACGNLLN"
     gene            complement(14277..15134)
                     /locus_tag="PKM27_12135"
     CDS             complement(14277..15134)
                     /locus_tag="PKM27_12135"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNR08059.1"
                     /translation="MVFLESIYSSRSGKKTYIAEYGLPARPHGLVILIHGLGEHIRRY
                     DEQFHYFVNKGYACLAADLPGHGRSEGRRGVWSSMDEPYAVIDRLFAIAAEKYPDTPR
                     ILYGHSMGANIAARYMQVRQPALRAAILTGIPIRTPKDMPRPLVRAILSSPVWIKNLT
                     IKNGLNIQSLCGDAQVVKNYLADPLVHDRVSLGAGASILDNAYHLTHTEWAPSFPVLV
                     MHGEDDKITFPEGSVLLKSVWNKNVDLKIWPGMMHEIHNEILKKQVWDHMLSWLDLLN
                     NQDTDNLSR"
     gene            complement(15142..15720)
                     /gene="pnuC"
                     /locus_tag="PKM27_12140"
     CDS             complement(15142..15720)
                     /gene="pnuC"
                     /locus_tag="PKM27_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNR08060.1"
                     /translation="MSLYVYLEWAAALAGLINVYLLTRQTILAWPAGLLSVFLYIFIF
                     YHHRLYSDVILHSVYVVLNLYGWYRWSRPDHQGNPLPVTRFSLGQNALLSLVILFSSL
                     AWGLFMKKNTHADFAYPNAFILVASLTAQYLMTLKKLENWVWWILVDITAIVIYSQKK
                     LLVTAVLYSLYLFLCILGFRAWYRSNKLTTLN"
     gene            complement(15754..17535)
                     /locus_tag="PKM27_12145"
     CDS             complement(15754..17535)
                     /locus_tag="PKM27_12145"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR08061.1"
                     /translation="MYKKYIYLPALFLLPWILSGATYYVSPQGNDGNSGLSPGNAFRT
                     LQQSAITVVAGDSVFVADSTYAGFDIRTSGTAADPIVFLALGSQVIINRRNPVTTDGI
                     NIEDADWIEIRGFQVINQPRAGIRAVNADHITIRDNTCLNNFKWGIFTGFTDDFLAEY
                     NECANSTDEHGIYVSNSSDRAIIRYNISHHNRAAGLHFNGDISQGEDGINHDPQVYGN
                     ILYRNGLGGGAAINMDGNQNALIYNNLIYDNYATGIALYQIDGGGPSINARIYHNTIV
                     QDPSARWCILLTDGASGATLRNNILINRHSFRGIINIDPASRTGFSSDYNVTTNWFTL
                     DDGNSVLDSSAWKALGYDTHSFFSANLTNLFINYPGEDLHLAPGAKAINFGITTLSPD
                     VATDLEGKLRTTGGAPDAGAYESVSGALPLFWTHVSIRRESHLIEIRGTLETDERAEK
                     IILEEWNQRTEAFTVRDSRTVSIAHGTLKTGFSLPASAGWHRYRLHAQNASGNKITSP
                     EIYIQVPTEDWSIFPNPTAGLIFLRLPDLDNASLVVRDAMGRTIRWIGNTREPVNLHG
                     LPPGKYLLTLMQDIHTLGQKWILKAGD"
     gene            17622..19463
                     /locus_tag="PKM27_12150"
     CDS             17622..19463
                     /locus_tag="PKM27_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNR08062.1"
                     /translation="MAIADNSGKDGTPASKPRMNWARLKESLYVFKYIRPYRWQFVGG
                     LILLALSSLLFMALLPLSGEMLDIALGKSAWKFSLTQMGLLLFLYLVIQGVISYFRVM
                     LFAIVSEKGTADLRKDLYHKLVTLPFPFYEKARVGEISSRLASDVDQLYAAFSITLAE
                     FIRQVIILVGGILFLLVSTPRLSIIMLAVFPVIVFGAMFFGRFIRKESKKRQEELANT
                     NVIVDETLQAIYTVKSFTNEPYEYHRYEKRITSLVGIALRLSRYRALFGTYIIIALFG
                     ALFFVIWLGAKYVQNGSMSPGDLLNVFTFTATIGVAIASLGSFYTQLVSVIGGTERLK
                     EILGKEAEVDMVQASSPSSVPMEGSIRFDRVEFSYPSRPDIDVLKGVSFSIGAGQKIA
                     FVGSSGVGKSTIAQVLLRFYEIKGGQILVGNKDYKDYEISEYRRHFGIVPQEVLLFGG
                     TIRENILYGKPDATEEELHRAARKSNCWDFINGFPEKFETIVGERGIKLSGGQKQRIA
                     IARAVLRNPTILILDEATSALDAESEKVVQDALKVLMEGRTSIIIAHRLSTIRDVDCI
                     YVLENGKIVEQGPHEILSAIPDGAYSNLARLQFEGNKELINPEENRA"
     gene            19472..20479
                     /gene="murB"
                     /locus_tag="PKM27_12155"
     CDS             19472..20479
                     /gene="murB"
                     /locus_tag="PKM27_12155"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNR08063.1"
                     /translation="MKILENHSLASLNTFGVEQKARYFATAQNTQEFLALVRQYENPR
                     ILGGGSNILITRDLDGLVIKNEIKGISIIGEDDRYARIRVCGGENWHEFVKWAVAKGW
                     GGIENLSLIPGTLGAAPIQNIGAYGVEIKDVLQVVEALDRTTGSIVHFQNAECRFGYR
                     HSLFKEPAQRDRYFILSATLQLDKQPQVNTRYADVERKLHELKIEHPNIRDVHRAVIE
                     IRQKKLPDPARLGNAGSFFKNPVLTEEQYRQLQSVKPDAPHYPFTHTFVKVPAGWLID
                     QAGWKGKQMGRVGCYEKQALVLVNLGGASGAEIWDFAQKVREDVQEKFGILLEPEINI
                     W"
     gene            complement(20488..21279)
                     /locus_tag="PKM27_12160"
     CDS             complement(20488..21279)
                     /locus_tag="PKM27_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR08064.1"
                     /translation="MAEKANYIHGVSPDEQTRLQLLNELTNQSFLDFLQLNGYEEVLE
                     LGSGLGILCAQVARLLHRGRAVGIELSKAQLGKAPRFPSNLEFVMGDVHELPFDDASF
                     DVVYGRYILEHISNPPGALKEAYRVLRPNGKIYFQENSILWMEFYPSCPHFMHAWKKF
                     AQLQNFLGGDAMIGIKLFDRLKKAGFRNLEISLAPELHTADSPHFKSWITNLIGNLRS
                     AEKKLMEKQFLNASEFLHAINELEEFMMHPQASTYFAWNRISGVK"
     gene            21408..23204
                     /locus_tag="PKM27_12165"
     CDS             21408..23204
                     /locus_tag="PKM27_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNR08065.1"
                     /translation="MPYSRLAGRKYLEDGLILVSTLAVLLVIINSGFSQTTHGLYSRW
                     FPVMNAVAFILLASRMSLNWKTRQLQVRWYAGQTFVLVLMLLALVGELFFHHILSGLQ
                     VTETLTANVFIYHSIFVFLFLEEVSTRLFSLKKSAVNAPFLLLISFLFFILVGSLLLM
                     LPSATTRPIPYVDALFTSTSAVCVTGLVVLDTATDFTLFGKWIILFLIQIGGLGILTF
                     TNLFGFIFSGSTTFQNQMLIQNMLNVQNLADALKALVRIVVITLIIEAVGGVVIFFLV
                     DSSIFSSPGEQAFFSIFHSISAFCNAGFSTLGNDNLYHESVRFAYGLHLVIAVLIIFG
                     GIGFGILLNILQYFREKVLQVYCLFIQRKPYRFKSQIITLNTRIVVFTTLVLLVVGMF
                     LFYLFEYHNTLRDHPTLFGKLVESFFASVTPRTAGFNVVSIAGLTIPTLMIYLLLMWI
                     GGSPGSTAGGIKTSVFAVATLNFINQVLGKERTELGRKEIPANSIQKAFAVISLSLMA
                     VGMSITLIILNDGGKFDLLKIGFEAFSALGTVGLSMGITPYLSDFSKIVLIFTMFIGR
                     VGFLTILIGLIRLFRREREKQYRYPKEDLFIT"
     gene            23218..23901
                     /locus_tag="PKM27_12170"
     CDS             23218..23901
                     /locus_tag="PKM27_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNR08066.1"
                     /translation="MKFIVLGLGNFGSELSIKLTELGHEVIGVDSNMKRVEYLQDSIT
                     HTICMDTTDQSAVRSLPVRNSDVVIIGIGEDTGASIITTALIKQMKANRLIARAINPA
                     HQQVMEAMGIDEIIHPEADTAYRLAKRLDIGTIVDSFTLDDQYKVIEATLPDAFVGKT
                     IREVDFQKEFNINIVTIVRNKEKVNNFGQVQVFHEILGVVSPETRLEARDMLVVFGKV
                     DDIDRMLKK"
     gene            complement(23916..>25583)
                     /locus_tag="PKM27_12175"
     CDS             complement(23916..>25583)
                     /locus_tag="PKM27_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08067.1"
                     /translation="QRTYSDVVFYNYKTGVKRTLTRKQKLFQPSPSNHSDGITCVELE
                     PSGLCHLIVLDTLGKVKHSIPVPDGYVATNPVFDAADTSLTAVNRGHGYAKLMRYTLS
                     DDKAHDLTGPVACIISNLVSQNDTVYFSSDVSGRDNIYAYDPVTQGVFQLTNHDLGIQ
                     QFNLEGDIILYNVQTAAGLRIRSMSISGSRMRRVDYFDKPSAPDSFTPARPASSLSLS
                     SKPAGPLNNPFRVYSWALRPDEGGNVFRIIGRNVLHTLQGTVAYKFLEEDRAQGLSGS
                     VALGNAYPLFTLSGGRTWGRRISSPSMVGTDSIRWDESNLSLGASIPLQFYRNNQIIQ
                     VRPSVQYGAYLPDFHKGDRVNYQSTQYLGAGLRLLNYRRRAYQHPASRFSQEVSIQWY
                     RAINHTAQALNLNTTFHFPGLGRNQVIETEWDFHRQPRSDAYRFSSTLRFISGYESFT
                     SDQASHWRVAYHFPLFYPDAGVNGIYFLKRVRGRLFHEGMHSLIRRTRGDLRVNYSSS
                     GAELLLDGQALNVLPVSFGVRFSYLWDRDLVSGSNRPQWSFVVEQLF"
BASE COUNT         6480 a         6354 c         6804 g         5945 t
ORIGIN      
        1 tataaaagaa aggtcctgga aaccctggac cttgatgcca tccggtttat cggttatgcc
       61 ttccagatag aaatgaaata caaatcctgg tataaaggat tcaaaataaa ggagattccc
      121 atcaccttca cggaccgggt ggccggcacc tccaaaatgc atggcgggat cataaaggaa
      181 gccgcactgg gagtgattca actcaggctg aaccggaaaa aataaggcaa ggccggaatt
      241 ttcatccccc tttttctcga tccaattcct caacggattg ccccggccgg gaaaaaaaat
      301 catttcattt tataagagct ttatttcata ttattgatat atgttatatt tatatatctg
      361 ttttattttt gtttatatca taattttaat tatgggttaa ttatatttaa tgtataaatt
      421 gcttatttac aatatttaat acatacaatg aaaattagat ttttaaaaaa tttgaaaaaa
      481 aagtaggtaa taatgggaaa aagtgggaca cttgatatat atttgagcta taaaccaatc
      541 agagagtgtc ccgctttaca ggagaatatc aaagcaccgt ggatgataag ggcagggtta
      601 aattacccgc aggccttatc cgccagatgg aggagcaggg catggttctg aattttgtca
      661 tcaatcgcgg ttttgaaaaa tgcctgatgt tgtatcccaa accggtttgg gatcagaaag
      721 cggcagaagt ggataagctc aatatctacg atccggtcca gaggcagttt gtccggtatt
      781 tttaccgggg ggccacccag atcctggccg atcctgccga ccgtcttctt cttcccaaaa
      841 accttactga atacgcaggc ttggaaaaaa acgtcacgct gtttgcctat ctcaatcaaa
      901 tcgagatatg ggatcagaaa aaatacaatg ctgaactggc aaacgagccg gatcagttct
      961 ccgaattggc ggcgaatgtt tttggagggg tccagccggc ttcgtcaata cccaaaggga
     1021 tttgatggaa taccatgtgc cagcgatggt acaggaatgc ctggaaggcc ttcatatcca
     1081 accggatggc atctatgtgg atgccacctt tggcggggga gggcattccc gggcgatttt
     1141 ggagaaattg ggcgtcaacg gacgcctgat cgcatttgac caggatgaac aagctttcca
     1201 aaacaggatc gcggatcgaa ggtttttgct gattcattcc aatttcagat atctcaggcg
     1261 gtttctgaaa tactatggga tcggccagat cgacggcctg ctggcggacc tgggggtttc
     1321 ctcatatcaa ctggacatgc ccggaagggg tttcgcattc cgccataccg ggccggacct
     1381 gcccctggac atgcgaatga accagcaaca gcccaggacg gcggccgatg tcatcaaccg
     1441 gacggacccg gcgaaactcg aaaagatcct gagggagtat ggagaggtct ggaacgcgca
     1501 ccggttggtg gaattgatca tgcaacgcag ggagcggaac ccggtgacga ccgtagccca
     1561 actggaggac ctggcaaggt ccgtggccgt cggcccggtc aaccaatacc tggctaaagt
     1621 gttccaggcg atccgcattg aggtgaatga agaaatgacc tctctgcgtc aattattgaa
     1681 tgacgcagta gaggtgttga agccgggagg caggctggtg atcctttcct accattccct
     1741 ggaggaccgg atggtcaaac atcttattca gaccggggat gtggatggac tcgtaaaaaa
     1801 ggatccgttt ggaaacaaaa taataaaact gagggcagtg tcaaaaaaat cgatccggcc
     1861 ctctgaagac gaaataaaag aaaactaccg ggtgcgcagt gcccgactac gaattgccga
     1921 aaagtcggca taatcactgc ggaaaaacaa tgcccagggc aaaccggagg tgtattcact
     1981 tccggagcag gggtatcatt tttaatcagg aaaatttatg gaagaaaggt ctcaaaataa
     2041 gtggctacaa caaatcctcg ccggcggaat ggtgatcaag cacctgcctt ttatctattt
     2101 cgccggcatt ttggccctgc tttatatcgc aaacgcccac caggcggagc ggaaggtcag
     2161 gaaaatccac aaactgagag aggaagtcaa ggaattgcgc tggcagtata tgtctttccg
     2221 gtcggaattc cttttttcaa gttcacccac ccagctcgcc aacgatgtca ggccccttga
     2281 actgggctac cataaacagt cctcgaccaa aaaacttgtg aagaaatagg ggatgaaaga
     2341 cgaaatcatc ataagaacct atgtcgtttt cctgctcttc gtacttgcgg gtttcctgat
     2401 cctgggccgg gtggtccaga tcggcatcgt tgaaggggag tcctggagag gccggggaga
     2461 cagtttgtat gtgaggcatc agcccataga accggaaagg gggaatatcc tttcctcgga
     2521 cggccgtatg ctggtaacct ccctgcctct gtttgaagtt cgtatggacc tgctggccgt
     2581 gccgaaggat aaattcgtaa aaaacattga ttccctggcc ctttgccttt cgcggcacct
     2641 caacccggtc atctccgaaa gagcctatcg cgacctgctg tggcgggaac gccaggctgg
     2701 caaccggtac ctgctgatta aggaagaagt gacctacagc cagctggaaa aaatcaagac
     2761 cttccccttg ttccggctgg gcggcaacaa gggaggattg atcgtggtgc gtaaatcaaa
     2821 gcgggagagg ccataccgga tgcttgcggc ccgcacactg ggttcggtgc gtgagggcgc
     2881 caaaccggta gggcttgaag ggtattatga cgatgtcctg ggtggtgaaa agggaagccg
     2941 gctggtccaa aaggtagcca gggacatctg ggttcccgtg gacgatatcg gggactttga
     3001 acccaaaaga ggaaaagata tcatcaccca tattgatatc gatctgcagg acatcaccga
     3061 acaagccctg tacaaatcgg tggcccacca ccaggcactg ggtggcactg ccgtggtgat
     3121 ggaagtgaag accggcgcaa tcaaagcaat ggccaacctg gtccgctcgg gagacgatat
     3181 cggcgagtac ctgaactacg cagtcggtgc agccacagag ccgggctcca ccttcaaact
     3241 ggctaccatg atggccctcc tggaagacgg gtatatccgt ctcgatgacc gggtggatat
     3301 tcatggagga gaggtggaat tgtgcggcaa aaaaatcagg gattccgaaa aacgcaaagg
     3361 gagcctgccg accctgaagc aggtatttgc acagtcctcc aatgtcgggg tggcggtgac
     3421 cgcacagaaa tattatgaaa aaaatgccaa aaaatttgtc aagcgactcg aacagtttgg
     3481 tctgaatgaa cggtcccgca tcgacctcga aggagaaccc gccccttata ttaaggaagc
     3541 ctacaacgtc gaacaaaaat ggagttgcac ctccattccc tggatggccc atggatatga
     3601 actgaccctg acgcccctcc agctcctgac tttttacaat gcggtggcca ataaaggccg
     3661 gatgatgaag ccccagttgg tacaccagat cctggagtat tcggagccgg taaaaaccta
     3721 taaaccccag gtgctgaaaa accagatttg cagccattcg accctccaac aggccatgga
     3781 attactcaaa agcacaacgg attccggagg cacggcttat gccctgcgca accggttgta
     3841 taaagtggcg ggaaaaaccg ggaccgccgt aaccaattat tacaaagggg atatcaacca
     3901 gggaaaaaaa taccaggctt cgtttgcggg cttttttcct gcggacgacc cggtttattc
     3961 atgtatcgtg gtcatttacg aaccctctgc caacggctat tatggggcct cggctgccat
     4021 gcccgtcttc cgggaaatag cggattacag tgtggcttcc cgcttcatcg cgacggagga
     4081 ccaagcgcct gtgaatacgg cgcccctgcc ggcccggatc cgtgcaggag gttatgctac
     4141 agacatcagg accctcctgc atttcagcgg cgtgaggttt gaaaaccgtt gcctcaatga
     4201 atgggccagc ctcagtccga ccgaaagcca gcctgaactg accgggatca acctccagca
     4261 ggatctggta ccggatgtcc gtggaatggg tttgcgcgat gcgctctttt tgcttgaaaa
     4321 taaaggatta aaagtcaggt tgcaggggca tggacgggtg agaagccagt cgattgcgcc
     4381 gggctccaaa attcaggggc agaccatcga actaactatg ggttgaagga tgaattggac
     4441 agaattaaaa aagcatatac agttctttga cattaaggga aacgcagaat ttgaagtggc
     4501 aggggtggag tcggattcac gaagagtgaa agaaaacttt gttttcattg cccagaaagg
     4561 gctggtcacg gacggtcacc ggtacatcgg cgctgcggtg gaacaggggg cgagggctat
     4621 cctttgtgaa agcttgccgg aagtattaag ccccggggtg gaattcctcc aggtgaaaga
     4681 tcccgccctg gcagccggga tcctggcgga tggtttttac ggccagcctt cctccaggct
     4741 caagttggtc ggagtcaccg gtaccaacgg taaaaccacc acggccacct tgctttaccg
     4801 cctcatgaaa gccctggggt tcaaggcagg tctcctgagc accgtgagaa actatatcca
     4861 cgaccgggcg atggaaacca gtcatacgac cccggatgtg atccggatca atgaaatgct
     4921 ggcgctcatg gtcggcgcgg gttgtgatta tgctttcatg gaagtgagtt ctcacgcgat
     4981 gaaacaacag cggacggccg ggctccggtt tgcgggcggg gtgtttacca atctaaccca
     5041 cgaccacctg gattaccacg gcacctttaa ggattacctg gattgtaaga agttgttttt
     5101 tgatcagttg ccccgggatg cctttgccgt caccaataaa gacgaccgca acggcatggt
     5161 gatggtgcaa aatacccggg cccgggtggt gacttatgcc ctgaaacaac tggccgaggt
     5221 caagggaaaa ataaaagcca ataccctcca ggggctggtg cttgaagtga atgggatgga
     5281 ggtgcacagc cgcctgatcg gcgctttcaa cgggtacaat tttctggcct gttttgccgc
     5341 cgcccgggaa cttggtttcg agccgatgga aatcctggcc ggcatgagcg gcttgccagc
     5401 cgtggatggg cggttcgata cggtttatca cgcggagaaa aaagtgcttg gcatcgtcga
     5461 ctacgcccat actcccgatg cattgacgaa tgtactcgat accattaaag aattgaaggc
     5521 ccctggttcc agggtgatca cggtagtggg atgcggcgga gaccgggaca aaaccaaaag
     5581 gccggtgatg gcccgcatag cgatggaaaa aagcgatgcc gtgattttta cctcggacaa
     5641 cccgaggacg gaagatccgc aacagatcct ggatgacatg atcgccggac ttgaacccca
     5701 gcaaatgcac cggatgatcc gacagtccga ccgcagggaa gccatcaaga gcgcagccag
     5761 gatggcggac cggccgggcg atatcatcct gatcgcaggg aagggccatg agacctacca
     5821 ggaaattcag ggaaagcgtt tcccctttga cgataaaaat atattaaaag aagttttaat
     5881 ataaaaaata taggttaatt atcattctca ggttggtttt aacaggtaca gcgggcgatg
     5941 gtctcaaaag tgaaatctta ccccgctttc ctgttttttt caatcccgga ggataactga
     6001 aaacaaatgc tgtaccattt attcgaatat atcaacaatc aggtcgattt acccggtgcg
     6061 ggcttgttcc gctatatctc tttccgggcc ggcggagcgt tgattttttc cttgttgatt
     6121 tccatgttgt ttggtcagcg catcataaaa tacctgcagg ccaaacaaat cggtgaaacg
     6181 gtaagggacc tgggtctcca tggccagaaa gaaaaagagg gtacgccgac catggggggc
     6241 atcatcatta tcctggccat cctcctgcct tgcatcttgt gggcaaggct ggacaatgta
     6301 tatgttttgc tgatgatcgc ggtgaccctt agtttgtttt ttatcgggta cctggatgat
     6361 tacatcaaag tgtttaaaaa gaacaagcag ggcctcaaag ccgtcaccaa actaaccggt
     6421 cagatcgccg tcggcctgat cgtggcgctt acgctgctat acaataaaaa cgtattaatc
     6481 cgggtcacgg aaaaagaagc cgtcgacaac cggtttgaaa tcctggagcg gctttccgcc
     6541 tcggccaatc ccggcaatcc cgaagtattg gtacatgccc gttccctctc caccaatgtg
     6601 ccctttacca aaggaaatgc ctttgattat gcgggcatcc tgcgctggac gggcgatgcc
     6661 gccaggaccc tgactccgct tctctatatc attatcatta tttttatcat tacggccgta
     6721 tccaacgcgg tcaatatcac cgatggtctc gacgggctgg ccacaggggt gtcttcgatc
     6781 gtgggcgtgg tcctgggcgt attcgcttac gtcagcggca actccatttt cgccaattat
     6841 cttggcattt attttatacc gaattcgggt gaactggtca tcttcacggc ctgcctgatc
     6901 ggggcctgta tcggcttttt atggtacaac gcttacccgg caaccgtatt catgggcgat
     6961 accgggagcc tgaccctcgg tggcatcatc gccaccctgg cggtggtttt gcgaaaggaa
     7021 ttgttgttgc ccgtcctctg cgggatcttt ttgattgaga tcctctctgt gatggtccag
     7081 gtttcctatt ttaaatatac ccgtagaaaa tacggcgagg gcagaaggat attcaaaatg
     7141 gccccgcttc accatcatta ccagaaaagc ggcatgcacg aatccaagat tgtaaaccgg
     7201 ttttggatcg tcgcggtcct cttggcagtg gtcaccatca tcaccctcaa aatcaggtaa
     7261 aatgctgcag atcacaacca agaaggaatg gaaaggcgat aaggtgatgt ggctcatcat
     7321 cgggatcctc atcggattcg gcctgcttgc cgtgtacagc gccacgggat ccatggctta
     7381 tctcagatac aaagggaata cggaagtcta cttgctcaaa caactggtca tggcagggat
     7441 cggcctgctg tgcatgtatg cctgccaccg gcagaattac ctgatctaca acaagctggc
     7501 cccggtcctg tttctgctgg ccatcccgct cctcctgtat accctggcct ttggcgtgga
     7561 aaccaacgat gccaaacgct ggattaccct cccggtcatc aatctttcgt tccagaccag
     7621 tgactatgcc cgcctggccc tcatcatgta cctggcccgc tcgctttcca ataaacagga
     7681 atacatcaag gactttaaga gcgcttttat accggtcatg gtgccggtcc tgctgatttg
     7741 cggactgatc gccccggcca acctgtcgac ggccctcttg ctttttgcga cctcggtcat
     7801 gctcatgttt gtcgggaggg tctccttcaa atatattttc atgatggcct tcttcggcat
     7861 cctgcttttc ggcaccgtgg tcctgatttg gtggtttttc ccggatgctg tgcgcgtcga
     7921 tacctggatc tctaggatca ccgattttat ttcgaatgaa aacggaggag gataccaggt
     7981 ccagcaatcc aaaatagcga tcgccaacgg gtcctggctg ggattggggc cgggcaacag
     8041 cacccagcgc aatttcctgc ctgcagctta tgccgactat atatacgcga tcatctgcga
     8101 ggaatacgga ttgatcgggg cgaccggaat actgctgctc tatctctggc tgttattccg
     8161 ggtatcccgg atcgtcatga aaagcccaaa agcttttggg gccatcctgg ctatgggatt
     8221 gaccctcaac ctggtggtcc aggcctttgc caacatggcg gtatccgtca acctggttcc
     8281 ggtcaccggc ctcaccctgc ccctggtaag tatgggcggg acttccgtgc tgtttaccag
     8341 cgtagccttt gggatcatca tgagcgtaag caaattcatc gaatccatcc aggaagaaca
     8401 gcctgctcct gaaaatgaac tgatgaagat ggcgaatacc agtccaaaac ctaaaaccgt
     8461 cgaagatgaa agtgatcatt agcggaggag gcaccggcgg tcatatcttt cctgccatag
     8521 ccattgcgca ggagcttaaa gcccgtgtgc ccggggtcaa catccttttc gtcggagcaa
     8581 gaggcaggat ggaaatggaa aaaatacccc aggcaggatt tgaaatcatc ggcctggatg
     8641 tggtaggctt caaccgttca tccctggtaa aaaatttcag tctgccctgg aaactggtca
     8701 aatcccttta ccaggcctgg cgcattttag taaaatacaa acccggtgtg gtggttggag
     8761 tggggggcta tgccagcggc ccggtcttgt acctggccaa ctggctccgg atcccgaccc
     8821 ttatccagga acaaaattcg tttgcaggta aaaccaatat gatccttggc aaaaaggccg
     8881 taaaaatttg tgtggcctac gatcatatgg agcggttttt ccccaaagaa aaaatcgtgg
     8941 tcacaggcaa tcccgtaagg aaagacctgg aaggcctgga atccaaaagg aaggaggcaa
     9001 tggcctattt cggcctcgat caggataaaa aaacaatcct ggtgatgggg ggcagcctgg
     9061 gcgcccgttc gatcaatgaa agcatgaccg cgggccatga acagatctca ggtcaaaaat
     9121 ccatccagtg gatctggcaa tacggaaaac aaggcgctga aaaattcggc cggtctgcaa
     9181 cagctggcct ggacaacgtg aaggccatgg cattcattga ccgggcggat ctggcttatg
     9241 ccgcagcaga tgtcttgttg tgccgggccg gcgcgctcac catatcggaa gtgctgatcg
     9301 caaacaaacc ggtggtactc gtgccctcgc cttccgtggc ggaggatcac cagacgcata
     9361 acgcgaaggc gctgtccgat gccggggcgg cttggctcct gccggacagg gaggtcgtgc
     9421 aaaatgggac ggcacaggtc atcaggcttt tgcaggacca ggaaacgatg acgcggatga
     9481 aacaagccca gaagtccctg gcccggccgc aggcggcggc agcgatcgtc gagcagattc
     9541 ttcatctcaa tcctgtggcc gcatgaaaaa attcctcaga aatatcggca ccgatcaaaa
     9601 catggtccgc ctgggcatgg gcctgaccgt gcttttgttt ctgatgtttt acgaaagggc
     9661 cggacgcttc aaaaacaggg ccctctccgg ccagatgcag gtccatgtac agcaccttga
     9721 aaacgggaac gatttcatca caacggaaga aatgcgttca aaaatcctcc gcaaattcca
     9781 gcaggaattt tccaacgtcc cgctggcgac gctggacctg caggagatcg aagcaggcct
     9841 gcaatccatg gactttatca aatccacgga cgtctacctg gatgccagca accggttaaa
     9901 catttttgta aaacaaagag agcccatcct tcgaatcctc actccggaag gaaacagttt
     9961 atacgtggat aaggatggaa gcatcatgcc ggcatccccg cattattcac ccagggtact
    10021 ggtcgccttc gccccggtac cgatgacgaa ggacagcctc gaattgagca agcccggcat
    10081 ggaaaaggac ctgtttgaac tggccaactt tatcgccagg gaccggttcc tgcacgccct
    10141 ggtggaggaa atcatggtcc atagtcagaa cgactggacc ctgatcccca aattcggtcc
    10201 ctcgcgcatt tttttgggtg acctggaaag gctggaggac aaaacggaca aactgaagcg
    10261 ggtataccgg gagatcctcc ctggccaggg atgggacctg tattattcgg ttaatctgaa
    10321 atttaaaggg caggtcatat gtacaaaaag agcataaatc aatctattta tcattctaaa
    10381 accaattaag atgaatgcaa gagactttaa taagaagaat gtggtggcgg ccctcgacat
    10441 cggcaccacc aaagtatgtt gcattgtagg cagaaaggac gaatacggca aaatcgacgt
    10501 gcttggcgcc agtaaggttg attctgaggg agtcctccgg ggagtcattg ccaatatcga
    10561 caagaccgtg aaagccattt ctgacgcagt ggaccttgcc gaaaaagacg ctcagtgcga
    10621 aatccaggag gtctatgttg gcattgccgg acaacacatc aagagcctgc aacaccgggg
    10681 catcctcgca agggacaata atgaaaaaga aatatcccgg gcagatatag accggctggt
    10741 gcaggacatg tacaaactgg gactgacccc cggggacaag atccttcatg tcattccaca
    10801 ggaattcacg gtggacaacg aacaaggcat cgtggatccc atcgggatgt cgggacaccg
    10861 gctggaagcg aatttccaca tcattaccgg gcagattgcc gcaaccaata atattatgcg
    10921 ctgcgtggag agggcaaacc tcaccgtagc cgatatgacg ctcgaaccca ttgccagtgc
    10981 cgaagccgtg ctcagcaagg aagagaaaga ggctggggtg gtcatggtgg atatcggggg
    11041 cggcactacg gacatcacca tcttccagga aggcattatc cgccatacgg cggtggtcgc
    11101 cttcggagga aacatcatca cccgggatat caaggaggga tgcactgtaa tgaccgaaca
    11161 ggccgaaaag ctgaaaatca aattcgggtc cgcccttgcc gacgaaatcg tggacaaccg
    11221 gatcatcacc atccccggac tgaaaggcag ggatcccaaa gaaatatcgg agaaaaacct
    11281 ggcgcgcatc atccaggccc ggctcgagga gatcctcgac ctgatcctgt atgaaatcag
    11341 aaggtccggg tatgagaaaa aactcattgc cgggattgtc ctcaccgggg gcggcgccct
    11401 cctcaaaaat tttgaattgc tttgtgaata ccataccggc ctgcaaaccc ggatcgggta
    11461 tcccaatgaa cactttggat acggattcaa ggataatatt tccagtccga tctatgccac
    11521 cggcgtcggc ctgctcatgt atggcctcaa gatgaaggaa tcccagcagc ggatgctgct
    11581 ccagacccgt aaagacagac cggccgaacg ccctgaaccc gtgacccagc ctgaattggg
    11641 cctgcccggg gtacccgcac ctaaaaccgc cagccccatc cctgcgcaag gcaaacaaaa
    11701 gaagccgctc gtggatttta tcaaaaactt tttcgaaccc tcaccggatc cggatctgcg
    11761 ctgatttatc cggagaagtg caagactcaa gattttaaaa tattaaaact ataaaccatg
    11821 aaattcgaaa taccaaaaga atcaggaaat atcatcaagg tgataggcgt tggcggcggg
    11881 ggcagcaatg ccgtgtccca tatgtaccgg caggggattg tcggagtgga ttttatcatc
    11941 tgcaatacgg acaaccaggc catggagacc agcccggttc cggtaaaaat ccagctgggt
    12001 ccccagctta ccgccgggcg cggcgcaggg tccaagcccc atatgggcaa acaagcctgc
    12061 attgaatccc tggaagaaat ccaggccctg atcaatgaaa ataccaagat ggtgtttatc
    12121 accgcgggca tgggcggcgg cacaggcacg ggagcggccc ccattgtggc caaaatcgcc
    12181 cgggaactgg atatcctcac cgtgggcatc gtcaccgttc ctttcccctt cgaaggcaag
    12241 cgcaggaagg agcaagccat tgaggggatc gaagaaatga aaaggaatgt ggatgccctt
    12301 atcatcatat ccaatgaaaa actccgccag atctatggca atctccccgt gagtgaagct
    12361 tttggcaatg cggacaatat cctgtccacc gcggcgaaag gcatcgctga aattattacc
    12421 atgcccggtt acgtcaacgt ggattttgag gacgtcaata ccgtcatgcg aaacagcggg
    12481 gtggcgatca tgggtaccgc ctccgcttcc ggggaaagac gggcttaccg cgccgtcgtg
    12541 gatgccctga gttcaccgct cctggaagaa aacgatattc ggggtgcgca gcatatcctg
    12601 ctcaacattt cttatggcac ccaggaagtc acgatggatg aaatatatga gatcacggag
    12661 tatgtccagg aagaggcggg ctatggtacc gatatcattt gggggaattg ttttgatgaa
    12721 aggcttgaag accagatctg cgtgacggtg atcgctaccg gatttgagca gaatttcggg
    12781 cataagcccg tggaggtccg tgagaaaatc aaggtttcgc tggaggagga aaaacccaca
    12841 acgtccctgc atgaggtttt tgatatcggg gaccagacca atgataaaac cattgagttt
    12901 gagcctgctt accgtgaacc catgattaaa aaccaggagg agccctttat ccgttcagaa
    12961 tccggtgcgg aaaattcagg tcgccacgaa gaagagaaaa gagcccgtcc gggcggttct
    13021 gaaacgaggt cggaaaccgg caggaaaatg aggttccaga tgcatcagat gcgcaatacc
    13081 tcggaaatgg aaaaccagcc ggcttacctc cgccgcaaca tcccgctgga agacgtgcct
    13141 ccttccaaag accatccaag ccccaaatgg actctttcca gcgatgacga accccagatc
    13201 cgggagaaca attcctatct ctacgacaat gtagactgag tttcgactgc agtatatctt
    13261 ttaattggtt ttgcacaaag tccggctgtc cttcgtaaag ggacggccgg ttttttttga
    13321 tctaaaggaa tggatccgcc cgaggcaagg ataaggtatg tcaggtttgc caaattaaac
    13381 ctgatttata aatctacctt ctcggccgag aagtgcatgg attaaaagac aatccggttt
    13441 gtaatggaaa atttattcca cggtttgggt tcagttgagc agattgccgc aggccctgca
    13501 ataaacggca tcggtttgat gaccttcttt aaaacaatgc gggcagacct ggttggaaaa
    13561 atgaatggat ttatcggttc ttgcatattc ttttgtaatc aggccggcgg gaacggcaat
    13621 gattgcataa ccaagtaaca caacaaatga agcaaggatt tgaccaaagg cggattttgg
    13681 tacgatgtcc ccatatccaa ggccggtgag agttacgata gcccaataaa cgctccgcgg
    13741 cagggaatcg aagttgggat tggcagggtg ttccagcatg taaatcaggg ccccgacaat
    13801 atttactaca atgaatatga aaaacaagaa gatgatgatc ttgtctttgg tgcgtttaaa
    13861 agcggtaatg agtatggcgc cctggtttac atagccggac atttcgaata accggaaaac
    13921 ccgtagcagg cgcaaggccc tcacaataat gattgcgtgc gtgcccgtta aaatcaggct
    13981 aaggtaggtg gggacgatgg cgatcagatc tatgatccca taagggctca gaatatattt
    14041 caatggttta ggggcaacga tcagccggag tatgaactca atggtaaaga ttacggtgaa
    14101 tccccattcc aggataaaga agaggttttg gatggatggc ggaaggttgg ccaaggtctc
    14161 caggttggca aggatgacac tcaccacgat aagcaccatc aggaccacat caaatttctt
    14221 accggcagga gtgtctgctt caaaaatggt ctcaaacagg tatttttggg ttggtttcat
    14281 cttgacaggt tatcggtgtc ttgattgtta aggagatcaa gccaggacaa catatggtcc
    14341 cagacttgtt ttttcaggat ttcattatgg atttcgtgca tcatccccgg ccagatcttg
    14401 aggtcgacgt ttttattcca gaccgacttt agcaggacac ttccttcggg gaaggtgatt
    14461 ttatcgtctt ccccatgcat gaccagaacg gggaaggacg gggcccattc cgtatgtgtc
    14521 aaatggtatg cattgtccaa aatggaagcg cctgcaccca ggctgacccg gtcatggacc
    14581 aggggatcgg caagataatt tttgaccacc tgggcgtctc cgcacaagga ctggatattc
    14641 aagccgtttt tgatcgtgag gttttttatc cacaccggcg atgaaaggat ggcccgtacc
    14701 aaaggcctgg gcatatcctt tggtgtccgt ataggtatcc cggtaagtat tgccgcccgg
    14761 agtgcaggtt gccggacttg catataccgc gcggcaatat tggcccccat ggagtgtcca
    14821 taaaggatcc ggggtgtgtc cggatatttc tctgcggcta tggcaaacaa ccggtcgatc
    14881 accgcgtagg gctcatccat ggatgaccat accccccgcc ggccttctga ccggccatga
    14941 ccggggaggt cggcagccag gcaggcataa cccttattca cgaaataatg aaactgttca
    15001 tcgtagcgcc ggatatgctc ccccaggccg tggatcagaa tgaccaggcc gtgtggcctg
    15061 gccggaagtc cgtactccgc gatataggtt tttttgccgc tccgggaact gtagatggat
    15121 tccagaaaaa ccattggaat tttaatttaa tgtagtcaat ttattcgatc tgtaccaagc
    15181 cctgaatccc aggatgcaca ggaacagata aagggaatat aaaacggcgg tcaccagcag
    15241 ttttttctgt gaatagatga cgatggccgt aatatcgacc aggatccacc agacccagtt
    15301 ttcaagtttt ttaagggtca tcaggtattg cgcggtcaga ctggccacca ggataaaggc
    15361 atttgggtaa gcgaaatccg catgggtgtt ttttttcatg aataaacccc aggccaggga
    15421 agaaaataag ataaccaggg acagtaaggc attctgcccc agggaaaacc gggtcaccgg
    15481 taaaggattg ccctggtgat cgggccggga ccagcgatac catccataga ggttcaacac
    15541 cacatagacc gagtggagga tcacatcaga atacagccgg tggtggtaaa aaataaaaat
    15601 ataaaggaaa acgctgagca atccggcggg ccaggccagg atggtttgcc gggtcagcaa
    15661 atacacattg atcagcccgg ccagggcggc tgcccattcc aggtacacgt ataaagacaa
    15721 gcctgaaagt tctgaaaaat accggaattg gtatcaatcc ccggccttga ggatccattt
    15781 ctgccccagg gtatggatgt cctgcatgag ggtgagcaga tacttccccg ggggaagtcc
    15841 atggaggttc accggctccc tggtattgcc gatccaccgg atggtcctgc ccatggcatc
    15901 ccggacgacc agggatgcat tatccagatc cggcagccta aggaaaatca aaccggcggt
    15961 aggattggga aaaatggacc aatcttcagt tggaacctga atataaattt ccggcgatgt
    16021 tattttatta cccgatgcgt tttgggcatg gaggcgatat ctgtgccatc ctgctgaggc
    16081 agggagactg aaacctgtct tcagcgttcc gtgggcaatg gaaacggtcc tcgagtcccg
    16141 gaccgtaaag gcttcagtgc gttgattcca ttcttcaaga attatttttt cggccctttc
    16201 gtctgtttcc agggtgcctc tgatttcaat gagatgggat tccctgcgta tggaaacatg
    16261 ggtccagaac aagggcagtg ctccggatac cgactcataa gcccctgcat ccggggcgcc
    16321 tcccgtcgtt cggagcttgc cctccagatc cgtagccacg tccggtgaga gcgtggtgat
    16381 accaaagttt atggctttgg cgccgggggc gagatgcaga tcctccccgg ggtagtttat
    16441 aaataggttt gtcaaattgg ccgaaaaaaa cgaatgggtg tcgtaaccga gggccttcca
    16501 ggccgatgaa tccaggaccg aattgccgtc atccagcgta aaccaattgg tcgtcacgtt
    16561 gtaatcgctg ctgaagccgg tgcgggaggc cgggtcgatg ttgatgatcc cccggaaact
    16621 gtgccggttg atcaggatat tgttgcgcag ggtcgcgccg gatgccccgt cggtcaataa
    16681 aatgcaccag cgggcggagg ggtcttgtac aatggtattg tgatagatcc tggcattgat
    16741 ggatggacct cccccgtcaa tctggtacag ggcgattccg gtcgcataat tgtcatagat
    16801 cagattgtta tagatcaggg cgttttgatt cccgtccata ttgatcgccg cgccgccgcc
    16861 caatccgttg cggtatagga tattgccgta aacctgggga tcgtggttga tgccgtcctc
    16921 cccctggctg atatccccat taaaatgaag gcctgcagcc cggttgtggt gactgatatt
    16981 gtatcttatg atcgcccggt cgctgctgtt ggaaacatat atgccatgtt cgtccgtcga
    17041 gttggcgcat tcattgtact cggcgaggaa atcgtccgta aatccggtga aaatacccca
    17101 tttgaaattg ttgagacagg tattgtcccg gatggtaatg tgatcggcat tcacggcccg
    17161 gattcccgcc ctgggctggt tgattacctg gaagcccctg atctcgatcc aatcggcgtc
    17221 ttcgatattg atgccgtccg tggtgaccgg atttctccgg ttgataatca cctggctgcc
    17281 cagggccaga aaaacgatcg gatcagctgc cgtgcccgag gtccggatgt caaatccggc
    17341 ataggtgctg tctgcgacga aaacggaatc ccccgcaacg acggtaatgg ccgattgctg
    17401 aagagtccgg aaggcgttcc cgggactcag gcctgaattg ccgtcattgc cctggggcga
    17461 tacataatag gtggctccgg ataatatcca gggcaataaa aacagggccg ggagataaat
    17521 atatttttta tacatatccg ttcgcttcaa acctgtaacg aggtcttgaa ttattttatt
    17581 tcaaatgagc gggggcttaa tacctaactt tgccctgctt tatggcaatt gcagataatt
    17641 caggaaagga cggaacaccg gcatcaaaac ccaggatgaa ctgggccagg ctaaaggaat
    17701 ccctgtacgt atttaaatac atcaggccct atcgctggca gttcgttggc ggcctgatcc
    17761 tgctggcttt gtccagcctg cttttcatgg ccttgcttcc tctaagcggg gagatgctgg
    17821 acatcgcatt gggcaaatct gcatggaaat tcagcctcac gcaaatgggc cttttgttgt
    17881 ttttatacct cgtcatccag ggcgtcattt cttatttccg cgtcatgttg tttgccattg
    17941 tcagtgaaaa agggaccgcg gacctgcgga aggacctgta ccacaaactg gtgacccttc
    18001 cttttccctt ttatgagaaa gcgagggtgg gggaaatcag cagccggctt gccagcgatg
    18061 tggaccagtt gtatgcggct ttttccatca ccctggcgga gtttatccga caggtgatca
    18121 tcctggtcgg ggggattctt ttcctactcg tatccactcc ccggctgtct atcatcatgc
    18181 tggcggtgtt cccggtgatt gtattcgggg ccatgttttt tggccgtttt atccggaagg
    18241 aatcaaaaaa aaggcaggaa gaactggcca atacgaatgt gattgtggat gagaccctcc
    18301 aggccatcta taccgttaaa tcatttacca atgaacccta tgaataccac cggtatgaaa
    18361 aaaggattac cagcctggtg ggcatagccc ttcggttgtc caggtaccgg gcgcttttcg
    18421 ggacctatat tattattgcc ttgttcggcg ccttgttttt tgtgatctgg ctcggcgcca
    18481 aatatgtaca aaatggttct atgagccccg gggacctcct taatgtgttt accttcacgg
    18541 ctaccatcgg cgtcgccata gcgagcctcg gatcctttta tacccaactg gtatcggtga
    18601 tcgggggcac ggaaagactg aaggaaattt taggaaagga agccgaagtc gatatggtcc
    18661 aggcctccag cccttccagc gttcccatgg aaggcagtat ccggttcgac cgggtggagt
    18721 tttcttatcc cagccggccg gatatcgatg tgctcaaagg ggtatccttc agtatcggcg
    18781 ccgggcaaaa aatcgctttt gtgggatcaa gcggtgtagg caaatccacc atcgcccagg
    18841 tcctcttgcg gttttatgag atcaaggggg gacaaatcct ggtgggcaac aaggattata
    18901 aagactatga aatctccgag tatcgaaggc atttcggcat cgtgccccag gaagtgcttt
    18961 tgtttggggg taccatcagg gaaaacattt tatatggaaa gccggatgcc acggaagagg
    19021 aattgcacag ggccgcccgg aaatccaact gttgggattt tatcaatggg tttccggaga
    19081 aatttgagac catcgtgggg gaaagaggga tcaaattgtc gggcggacaa aaacaaagga
    19141 tcgccattgc ccgcgccgta ttgagaaacc ccaccatcct catcctcgac gaagccacct
    19201 cggccctgga tgcggaatcc gaaaaagtgg ttcaggatgc attaaaagtc ctgatggaag
    19261 gacgcacctc gatcattatc gcccaccggc tctctaccat ccgggatgtc gattgcattt
    19321 atgtactcga aaacggaaag atcgtcgaac agggtcccca tgaaatcctg tccgccatcc
    19381 ccgacggggc atacagcaac ctggcccgcc tccagtttga gggcaataaa gaattgatca
    19441 atccggaaga aaaccgggcc tgaggccgtc tatgaaaatt ctggaaaacc attcactggc
    19501 atcgttgaat accttcgggg tggagcaaaa ggcgaggtat tttgccacgg cacaaaacac
    19561 gcaggaattc ctggccctgg tccggcagta tgaaaatccc cggatcctgg gaggggggag
    19621 caatatcctg atcacgcggg acctggacgg cctggtcatt aaaaatgaaa ttaaaggcat
    19681 atcaatcatc ggggaagacg accggtatgc ccgaatcagg gtatgcgggg gagaaaactg
    19741 gcacgaattt gtaaaatggg cggtggcaaa gggttgggga ggcattgaaa acctctccct
    19801 catcccgggt acccttggag ccgcgcccat ccagaacatc ggcgcttacg gggtggagat
    19861 caaggatgtc ctgcaagtag tcgaagccct cgatcggact accggatcga tcgtgcattt
    19921 tcaaaacgca gaatgccggt ttggttaccg gcacagccta tttaaggagc cggctcagag
    19981 ggacaggtat tttatcctca gcgccaccct gcaactggat aaacaacctc aagtcaatac
    20041 ccggtatgcg gacgtggaaa ggaaactgca cgagctgaag atcgaacatc ccaatatcag
    20101 ggatgtgcac cgggcggtca ttgaaatcag gcaaaaaaaa ttgcccgatc ctgcccgcct
    20161 gggaaacgca ggttcatttt tcaagaatcc ggtgctcacg gaggaacaat accggcagct
    20221 gcaatccgta aaaccggatg cgccgcacta tccctttacg catacgtttg taaaagttcc
    20281 cgccggctgg cttattgatc aggcgggttg gaaaggaaaa caaatgggca gggtaggctg
    20341 ctatgaaaaa caggccctgg tcctcgtcaa cctgggtggg gcaagcggag ccgaaatctg
    20401 ggattttgcc caaaaagtga gggaagatgt tcaggaaaaa tttggaatct tgcttgaacc
    20461 cgaaatcaat atctggtaat ccgcgcttta tttaacaccg gagatccggt tccaggcaaa
    20521 ataggtggat gcctggggat gcatcataaa ttcctccagt tcgttgatgg catgcaggaa
    20581 ttccgatgca ttgaggaatt gtttttccat cagttttttc tcggcgctcc ggagattacc
    20641 gatgaggttg gtgatccagg atttgaaatg aggggaatcg gcggtatgga gttcgggggc
    20701 cagggaaatt tccagattcc tgaatcccgc ttttttcaac cggtcgaaca atttgatgcc
    20761 gatcatggca tccccgccca ggaaattctg caactgggca aattttttcc aggcatgcat
    20821 aaaatgcggg caggacgggt aaaattccat ccacaggatg ctgttttcct ggaagtagat
    20881 tttcccgttg ggcctcagga cccggtaagc ctctttgagg gcgccgggag ggttgctgat
    20941 atgttcgagg atataccttc catagactac gtcaaacgag gcatcgtcaa agggcaattc
    21001 gtggacatcg cccatgacaa attccaggtt ggaggggaac cggggggctt tgcccaactg
    21061 ggctttggac aattcgatgc caaccgccct gccccggtga agaagccggg ctacctgtgc
    21121 acaaaggatg cccaggccgg accccagttc cagtacttct tcataaccat tgagctgtaa
    21181 aaaatccagg aaggattgat tcgttaattc attgagcaat tggagacgcg tctgctcatc
    21241 gggactgacc ccgtgtatat aatttgcttt ttcagccaca accacgctta ttaaaaaata
    21301 attcttaaat atctattaaa atcctgagat ttgcaagctt ctatccttca catttgtgaa
    21361 acaaagcgct ttaaacagcc atcggctttt ccactgccaa aaccgatatg ccctactcaa
    21421 gacttgccgg ccgtaaatac ctcgaggacg gcctgatcct ggtttcgacc cttgcggtat
    21481 tgctggtgat catcaattca ggattttccc agaccacgca tggtctgtac agccgctggt
    21541 tcccggtaat gaatgcggta gcctttattc tgcttgcctc gcgcatgtcc ttaaactgga
    21601 agacccggca attgcaagtc aggtggtatg caggccaaac ctttgtcctc gtcctgatgc
    21661 tcctggccct ggtgggagag ttatttttcc accacattct ttccggcctc caggtgacgg
    21721 aaacgcttac cgccaatgtg tttatttacc acagtatttt tgtttttctg ttcctggagg
    21781 aagtttccac ccggcttttt tcgctgaaaa aatccgccgt caatgcgcct ttcctcttat
    21841 tgatcagctt cctttttttt atactggtgg gcagcctgtt gctgatgttg ccatccgcta
    21901 ctacgcggcc gatcccttat gtggatgccc tgtttacttc cacttccgcg gtctgtgtga
    21961 ccgggctcgt ggtgctggat acggcgaccg attttacttt gttcggcaag tggatcattt
    22021 tatttttaat tcagatcggg gggctcggca tattgacctt caccaatctt ttcggtttta
    22081 ttttcagcgg atccaccact ttccagaacc agatgctgat ccagaatatg ctcaatgtgc
    22141 aaaacctggc ggatgccctc aaagccctgg tccgaatcgt ggtcatcacc ctgatcatcg
    22201 aagccgttgg gggggtggtt attttctttt tggtcgattc cagcattttt tcgtcccccg
    22261 gggaacaggc ctttttctcc atttttcatt ccatcagcgc tttttgcaat gccggttttt
    22321 ccaccctggg caacgacaac ctgtatcatg aaagtgttcg gtttgcttat ggcctccatc
    22381 tggtcattgc ggtgctgatc atattcggag ggatcggttt tggcatcctg ctgaacatcc
    22441 ttcagtattt ccgggaaaaa gtactccagg tctattgcct gttcattcaa cgaaagccct
    22501 accggtttaa atcacagatc atcaccctca acacccggat tgtggtattt acgaccctgg
    22561 tattgctggt ggtgggcatg tttttgtttt acctgtttga atatcacaat acccttcgtg
    22621 accatccgac cttattcggg aaactggtgg aaagtttttt tgcctccgta acgcccagga
    22681 ccgccggatt taatgtggtc agcattgcgg gattgaccat tccaaccctg atgatctacc
    22741 tcttgcttat gtggatcggc ggatctccgg gaagcacggc aggggggatc aaaaccagcg
    22801 tgtttgccgt ggccaccctt aattttatca accaggtcct gggtaaagaa aggacggagt
    22861 tgggcaggaa ggaaatcccg gcaaattcga tccaaaaggc tttcgcggtc atcagtctct
    22921 ccctgatggc ggtggggatg tcgatcaccc tgatcatcct gaatgacgga ggaaaatttg
    22981 acctcttgaa aatcggtttt gaggcttttt ccgcactggg cactgttggc ttgagcatgg
    23041 gtattactcc atatttgtcg gatttcagca agatcgtttt gatttttacg atgttcatcg
    23101 gccgggtggg atttttaacg attctgatcg gtctcatccg gctttttaga agagaaaggg
    23161 aaaaacaata ccggtatccg aaagaggatc tgttcatcac ctgattaatt ttattctatg
    23221 aaatttatcg ttttaggcct tggcaatttt ggttccgaac tcagcatcaa gctcaccgag
    23281 ctgggtcatg aggtcatcgg cgtagactcc aacatgaaac gggtggaata tttgcaggac
    23341 agcatcaccc acaccatctg tatggacacc acggaccagt ccgccgtccg ttctttgccg
    23401 gtaaggaatt ccgatgtcgt catcataggc attggcgaag acaccggcgc ctccatcatc
    23461 actaccgcac tgatcaaaca aatgaaagcc aatcgcctga tcgcccgggc catcaacccg
    23521 gcacaccagc aggtcatgga agccatgggt atcgatgaaa tcatccaccc ggaagccgac
    23581 acggcttacc gcctggccaa aaggctggat atcggtacca ttgtggattc tttcaccctg
    23641 gacgatcagt ataaagtgat cgaagccacc ctgcccgatg cctttgtggg gaagaccata
    23701 cgggaagtcg attttcagaa agagtttaat atcaatatcg tcaccattgt gcgcaacaag
    23761 gagaaggtga ataatttcgg ccaggtgcag gtcttccatg aaatcctcgg ggtcgtctcc
    23821 ccggaaaccc gcctggaagc cagggatatg ctggtcgtct tcggtaaagt ggacgacatt
    23881 gaccggatgc taaaaaaata ggacctgttt ctatcctaaa acaattgctc caccacaaaa
    23941 gaccattgag gccggttcga accgctcacc aggtcgcggt cccacagata actgaaccgg
    24001 acgccaaagc tcaccggcaa tacattcagc gcctgcccat ccaacaacaa ttccgccccg
    24061 ctgctggaat aattgacccg gagatcgccc cgcgtacgac ggatcaagga atgcatccct
    24121 tcatgaaaaa gccggcctct tacccgtttt agaaaataga tcccgttgac gcccgcgtcc
    24181 gggtaaaaga gtggaaaatg ataagcgacc ctccaatggg aggcctggtc gctggtgaaa
    24241 ctttcataac cggaaataaa acgaagggta ctggaaaacc ggtaagcatc ggaccgcggc
    24301 tggcgatgga aatcccattc cgtttcaatc acctgatttc tccccaatcc cggaaaatga
    24361 aaggtggtgt tcaggttcag ggcctgagcc gtgtgattga tcgcccgata ccattggatc
    24421 gatacctcct gggaaaaccg ggatgcggga tgttggtagg cccttcgacg gtaattgagt
    24481 aatcgcaagc cggcgcccag gtactgcgta ctttggtaat tgacccggtc gcctttgtgg
    24541 aagtccggca ggtaagcccc gtattgcacc gagggcctga cctggatgat ctggttgttg
    24601 cggtaaaact gaagggggat ggacgcgcca agacttagat tggattcatc ccaccggatg
    24661 ctgtccgtcc caaccatgga cgggctggat atcctgcgtc cccaggtcct tcccccgctc
    24721 aaggtgaaga ggggataggc attgcccaag gcgaccgacc ccgacaaacc ttgcgctctg
    24781 tcttcctcca aaaatttata ggcgacagtt ccttgcaagg tatggaggac atttcgccca
    24841 atgatcctga aaacattacc tccttcatcc gggcgcaggg cccagctgta aacacggaat
    24901 gggttattca ggggacctgc aggtttggac gacaaggaaa gggaggaggc aggcctggcc
    24961 ggtgtaaaag aatccggggc agaaggtttg tcgaaataat ccacccgccg catccggctg
    25021 cctgaaatgg acatggaccg tatccttaat ccggcggccg tctgtacatt gtatagaatg
    25081 atatcccctt cgagattgaa ctgctggata ccaaggtcat ggttggtcaa ttgaaacacc
    25141 ccttgcgtga ccgggtcgta ggcatagata ttgtccctgc cggatacatc gctgctgaag
    25201 taaacggtgt cgttctggct aacgaggttc gaaataatgc aggctaccgg cccggtgagg
    25261 tcatgagcct tatcgtcgga cagggtataa cgcatgagct ttgcatagcc atgtccacgg
    25321 ttgaccgcgg taagacttgt atccgccgca tcgaaaaccg ggttggtggc gacgtatccg
    25381 tcaggcaccg gaatggaatg tttcaccttg cccagggtat ccaggacgat cagatgacac
    25441 agacctgagg gctccagctc cacacaggta atgccatccg aatggttgga tggagaaggc
    25501 tgaaacagtt tttgttttcg ggtcaacgtc cttttaaccc cggttttgta attgtaaaaa
    25561 acgacatcgg aataagtccg ctg
//
LOCUS       DAOQXT010000067        25539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2257_length_25539_cov_6.783001, whole genome shotgun sequence.
ACCESSION   DAOQXT010000067 DAOQXT010000000
VERSION     DAOQXT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 25539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25539
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_25539_cov_6.783001"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKM27_12180"
     CDS             complement(<1..301)
                     /locus_tag="PKM27_12180"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNR08068.1"
                     /translation="MKRIGVFTSGGDAPGMNACIRAVVRTALHYKMEVFGINRGYSGM
                     IRGDMYQMTSRDVSNIMQLGGTILQSARSETFRTPSGRRRAYNQLKKAGIEGLVAI"
     gene            complement(305..1078)
                     /locus_tag="PKM27_12185"
     CDS             complement(305..1078)
                     /locus_tag="PKM27_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08069.1"
                     /translation="MDLIKAKSIMARLQAMINTLEANPDLSALERDLILNYLRELYEL
                     VNDIRPQSLPVFKPKQEAIPTVPEKELGQKTLEFKFEPIEEPAVEKPAEKIIPPPPPQ
                     PEPIPVEAAVVEAIKPVNEPVETNPAPASAPAPAPSEPAPVPEPVQAKPNPMPASILE
                     LFEVRRGSELSDKLNELPLRDINRAIGIADRMEIIQTLFGGQRQLFDQIITDLNQFKS
                     YEEAREFLGPGPAIHYKWDHDDRREKAVEFIRLIRRRYL"
     gene            1181..1366
                     /locus_tag="PKM27_12190"
     CDS             1181..1366
                     /locus_tag="PKM27_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08070.1"
                     /translation="MISKKGEKEKRRKGGRNLNLSLNLNLNLNLNLNLNLNLNLNLTL
                     HLHLFFNFALHVFGTPF"
     gene            1344..1937
                     /locus_tag="PKM27_12195"
     CDS             1344..1937
                     /locus_tag="PKM27_12195"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNR08071.1"
                     /translation="MFLEPHFKGQRSGWIEVICGSMFSGKTEELIRRIRRAKIANQKV
                     VIFKPSSDVRYAESQVVSHDQNTIESVPIRHSKEILKLSAGAEVIGIDEAQFFDLDLP
                     EVCQQIALRGIRVIAAGLDMDYLGRPFGPVPNLLAIAEYITKVHAICPHCGNLATHSY
                     RLTEEKDTVVLGEKDKYEPRCRTCYHMGNILNFQRDR"
     gene            1993..3525
                     /gene="purH"
                     /locus_tag="PKM27_12200"
     CDS             1993..3525
                     /gene="purH"
                     /locus_tag="PKM27_12200"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNR08072.1"
                     /translation="MSKIRIRSALVSVYHKNGLDDILHLLEKMEVTIYSTGGTYQFIQ
                     GLGIPVVSVEQLTGFAEILDGRVKTLHPKVFGGILARRDEGHLAQLEAQEIPEIDLVI
                     VDLYPFEKTVQNTTVESEIIEKIDIGGISLIRAAAKNYKDVLIVPSQEDYAFLHELLV
                     TKEGYSDLADRKFLATRAFQVSSHYDTQIFRHFNRDEKVQTLVFSAPLDRTLRYGENP
                     HQAAGFYGHLDRLLQALQGKELSYNNLVDVDAAIWLMKDMDEFGSSVAILKHTNVCGM
                     ASRDTLVQAWTDALAGDPVSAFGGVIITNRTVDEETASAIDHLFYEVLIAPDFDFAAL
                     RLLQKKKNRILLKLNQWPEEKWMVKSLLNGLIRQERDLALTTKAQLKSVTVHTANEQE
                     LTDLVYANRLVKHLKSNAITLVKNKQLIGMGCGQTSRVDALKQAIGKARHFGFSDLLA
                     GSAMASEAFFPFPDCVEIAHEAGIRNVVQPGGSVKDQESIDFCQTHGMAMYLTGIRHF
                     KH"
     gene            3528..4262
                     /locus_tag="PKM27_12205"
     CDS             3528..4262
                     /locus_tag="PKM27_12205"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNR08073.1"
                     /translation="MDLFLCLDAGNSRQKLAVFHGERMMYFQVEKEFSIEQLTKVLDR
                     FPVSAAMLCEVKSLNKPIKNFLRKKVRLLEMSHRLRLPVRNEYRTPETLGKDRLAAAA
                     GAVRLYPGEAVLIIGLGTCITYDFVSGRGVYRGGAISPGLDMRFRAMHDFTEKLPLVK
                     FDPKAALLGNSTTTALQAGAVWGMTLELQGMIERYRKKYKNLKVILAGGGADFFKKRL
                     KKPIFAHPNLVLLGLNEVLSLNVLET"
     gene            4246..5583
                     /locus_tag="PKM27_12210"
     CDS             4246..5583
                     /locus_tag="PKM27_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08074.1"
                     /translation="MYWKLRLPGVLLLVLSFCRLTAQEKPREYSPYSRFGFGDLTEPH
                     FSALGQLAGIAAGFHDRNMLNYSNPAALGSLRNTAFEGGVSTKFTTLKNQDLSKDFFG
                     GGLDYLALGVPIFNPINEALDRLERKFHWGMLVALKPFSHVGYDVSQIDSITGTGRYE
                     RRYTGSGGTYEFKWSNGFEYRNFSAGLGLGYLFGRMSYRRNLDLLAVSLPYSTIFLDE
                     ISVKGFRWNAGLQYRINLNKPVEGESESTKTLTFGLYGQTSQSYNTISDHLYRRQLIV
                     SAQVGVVDTVRDEKEIKGRGKLPGEFHLGVFYSDRYKFQAGGQLSLVSWSAYTNDAKP
                     EILDNSSQFAFGLSWCPDISSLDNLLKRITYRVGFKAGNDPRSFDGEQLKNYGVTFGV
                     GVPFVFQRKVSFANFAVETGSMGLKDKLKSNYLQLKLGFTLNDDEWFLKRKYN"
     gene            5612..7036
                     /locus_tag="PKM27_12215"
     CDS             5612..7036
                     /locus_tag="PKM27_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08075.1"
                     /translation="MKKSLYTIALVWLVSSSLSSSLNAQCNTWLNSPQKDEAETAHTL
                     YRDAIKAGKIMEAIPNWEKAFNIAPAADGLRDWHFSDGITIYKAIYEKETDAAKKEAA
                     LKKVMELYAGMIECYKAKSIKIPNCNDEACLQKKIGDIKALQAYDMYYSLRNPYPEVL
                     KVLAECTKISGKDALYTIFVPYADIAVYQFLDKALPKEEARAIHQKLNEIGEHNVATN
                     KKYGSYYKDALAYANAKFAEIEDFIFDCDYFKEKLEPEYRKDAENHETIKMVFNKLSS
                     KGCTETDPLMIELKTKFEKIVAEENARRRAEFEASNPIYMAKKMIDEGDFDGAIAKYK
                     EAIESAETDDEKGELVLSIASVQFRKLGQAAAARESARKAAGLKDNWGAPYLLIGDMY
                     VSGARNCDDDWGSRLAVLAAIDKYQTAKSIDPSMTSEANERIGRISSAMPAKDDGFMR
                     GHKEGQVLTVPCWIGESVRLRFKD"
     gene            7128..8567
                     /locus_tag="PKM27_12220"
     CDS             7128..8567
                     /locus_tag="PKM27_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08076.1"
                     /translation="MMLKNTFPVLLLAILSCSPKTQLPAPPPAAPQPASPAVDLSNLS
                     PCPNWLSLPNQDDITNEYIIYRDALKAGNYSRAFEGWKTVYAVAPAADGKRSTVLDDG
                     IRIYTHLFNQEKDETKRKEYVQKVLDLYDQIGTCYPTGANVDAQKAFQYYYSFPGYAT
                     DEQIFTLFKKALDQDGLKADYFILNPFTALLIQRFVDNKITLEETQKYAGLVRSILQE
                     GLKNCKTPAACEPWNIINEYVPARLEDLEGVEGFYDCTYYKNKYFRDFQAAPTDCEAI
                     NTVYGRLRWAKCPDTDEAVLALRAARDQNCRVVVEVKETTLSKAIDALNEGNYRQSVE
                     LLKQAIEVTEDPKRKAELAMTISKVYYGNLRNFPESRRWALQAAEYRPNWGEPYLTIG
                     KLYASSGPLCGPGRGYDSQQVVWPAIDKWNYAKSIDPTTAAEANKLINQYLQYMPTRE
                     DLHMRSISEGENIRIGCWINETTKTRTIK"
     gene            complement(8571..8864)
                     /gene="trxA"
                     /locus_tag="PKM27_12225"
     CDS             complement(8571..8864)
                     /gene="trxA"
                     /locus_tag="PKM27_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNR08077.1"
                     /translation="MQFSQLINQEKPVIIDFYADWCGPCKMMAPMLEQIKDEYDDQIK
                     IYKIDIDKNRSLTEKYQVQAVPTVMIFKSGKLGWRVAGVPSIQEVRNQVNKLL"
     gene            8940..9548
                     /locus_tag="PKM27_12230"
     CDS             8940..9548
                     /locus_tag="PKM27_12230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNR08078.1"
                     /translation="MNKSIFFLLLMVLAIACQSKKTDTPAEETQVTEASPAEATETAP
                     AAASLSPEIWNSELGARLKADYKENPANQAETDQNLILEYAVQNNLDVKRTPSGLYYI
                     MEKEGVGKSPKSENVVSVNYRGYFLDGKEFDSSYKRNAPIQLPANQFVPGWTEALTMM
                     KPGGKMKVLIPSHLAYGPVGYPGAIPGNAVLGFDMELLKVVQ"
     gene            complement(9607..10551)
                     /locus_tag="PKM27_12235"
     CDS             complement(9607..10551)
                     /locus_tag="PKM27_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNR08079.1"
                     /translation="MYKRILANDGIEADGKTALEVAGFTVDTNKVEQADLAKALQQYE
                     VIVVRSATKVRKDLIDACPHLRIIARGGVGMDNIDVDYARSRNVTVINTPKASSRAVA
                     ELAIGHMFALARMLQQSNFQMRAEGADFNALKKNYSSGSQLLGKTLGIIGFGRIGQEL
                     AKIALGLGMKVIAADPMIQTAELKFEIGSHSFSLSVPTDTLENLLIRSDFVSLHVPSS
                     QKPLLGREEFGKMKKGACVINTARGGTVDETALLEALESGQLGGAGLDAFLNEPNPSI
                     EILRHPKISVTPHIGGSTREAQAAIGKELAEKIIAQYA"
     gene            complement(10709..11077)
                     /locus_tag="PKM27_12240"
     CDS             complement(10709..11077)
                     /locus_tag="PKM27_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08080.1"
                     /translation="MPRPYKTTGCFRFFLFLIILAPLAYIGASYYHGQDGIGNIVNLF
                     KKGLSVFSSSDSKKSSETTTSLPVESPGAREGEKIKQSDLENSLEIKDRQIESLIKEN
                     LELKQKLKEYEEAAKKTPNK"
     gene            complement(11186..13297)
                     /gene="recG"
                     /locus_tag="PKM27_12245"
     CDS             complement(11186..13297)
                     /gene="recG"
                     /locus_tag="PKM27_12245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNR08081.1"
                     /translation="MLVRPDYLKSPIEYLKGVGPVKADLFKTELKIFTFADLLYAFPF
                     RHVDRSAITPIRQIRNDGQAVQVRGILSALSFSGTKFQKRIKASIQDQSGVLNLIWFQ
                     GSQWLEQSLRPGQEYLAFGNLTGYGYPFTMAHPELDLLSELVRMPGFEPVYNSTEKLN
                     KKGLDSRMRRKLITTLIENLPPGALPETLPDYLVRSMNLCSRAEAIRWIHIPSSRNEL
                     ERATQRLKFEELFFQQLKLLTTKSFRKKHLKGPVFEKVGTSFNEFFRNHLRFELTEAQ
                     KRVIKEIRHDTASGAQMNRLLQGDVGAGKTIVGLMIMLLAIDNEYQACLMAPTEILAT
                     QHYASITYALKGLGLPVGLLTGSVKGEKRKELLRLVRQGDIKILIGTHALLEDPVRFH
                     NLGLAIIDEQHRFGVAQRANLWQKNEKISPHILVMTATPIPRTLSMVYYGDLDVSIID
                     QLPPGRKPVKTMHYNEKSRPQVYQFLKDQIARGRQIYIIYPLIEESEKLDLQDLQNGY
                     EQLLHHFPRPAYQISVLHGRMRPEDKDFEMQQFSRGKTHILVATTVIEVGVDVPNASV
                     MVIENAERFGLSQLHQLRGRVGRGADQSYCLLMTGYKLSKEAKIRLKTMCETTDGFRI
                     AEVDLELRGPGELEGTRQSGGLDFVMVNLVTDLQILDQVQKMVAKIIERDPELIHPSN
                     KLLLEYLHQEELRSPGWSKIS"
     gene            13523..13906
                     /gene="rbfA"
                     /locus_tag="PKM27_12250"
     CDS             13523..13906
                     /gene="rbfA"
                     /locus_tag="PKM27_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNR08082.1"
                     /translation="MESKRQQQVAELILRNFSYVLQQEGLYIYGREPLVTVTSVKVAS
                     DLQLARIYLSIYNTENKEAILGQIREHDHKLKQEMYHRLKKQLRRMPALEYFIDETLD
                     EMYRVEALFQRLEGQKQAPGTGAGE"
     gene            complement(13907..15166)
                     /locus_tag="PKM27_12255"
     CDS             complement(13907..15166)
                     /locus_tag="PKM27_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNR08083.1"
                     /translation="MDIVEILQSAWAMRALLAGSLVGITCGLLGCFIVLRNMSLIGDA
                     LSHSILPGVVLAYVLVGQNLLGVFIGSLAAGIVTVVLMTWFQQNAKTKNDAVIGIVFT
                     AMFSIGVMGISYLSHTPGFHLDLKDFLFGNILGIQDSDLQLTVLISVVVILGLVFLFR
                     PLFITTFQPTIAFTQGIRVKSVHYALMILLSIVVVSALQTVGVILVVAMLITPASTAL
                     LLSDRLHRVLVLSGLIGLVSAVLGLFLSIYAQTTPGPAMAVMATLIYVLAVFFAPRKG
                     LIKRWQRQRRQKLKVDLEDILKESFKLQEAGELGLKTLITKISIPAPRLKKLLGYLNR
                     RNQIILTPDLSLTPEGISRAQALVRAHRLWETYLVNQIGLTEDQIHDEAEYQEHHSPD
                     DLLDEIDAKLGYPSIDPHGSPIPPKKG"
     gene            complement(15234..15677)
                     /locus_tag="PKM27_12260"
     CDS             complement(15234..15677)
                     /locus_tag="PKM27_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08084.1"
                     /translation="MKIIHGWICLGLLVSCNSGPSKPKVPPESMAYLNLQQIMALSEQ
                     ADFVDFLFFNMDVSVSQSDKASISQTVNFFDNQAKPEGMNCPAIGRISFQSGGKIIRE
                     ADIHFLGTGCGYFTIIENKKPVGTNLISEPGLKFFETLVTNYKPK"
     gene            complement(15692..16351)
                     /locus_tag="PKM27_12265"
     CDS             complement(15692..16351)
                     /locus_tag="PKM27_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08085.1"
                     /translation="MYRLGFFLFLITSGIPSGSNAQALRFGSGIFAGLNLSQIDGDDM
                     QGYDKMGINAGLRGIAYIIPKLEFHTELAYNQRGSQSKGFSQSNDKGRKMILDYASIN
                     ALVVLNDWFHPIKEYYRIQACAGVGTGRLIRSNVYDPAGDRDQRQIPLSHLPPFLNTT
                     DFSLILGINVKVDEQAGLSFRFNRSMNKLLDSDLVPTINERRVYSMTGYFISCDFFYH
                     F"
     gene            16507..16797
                     /locus_tag="PKM27_12270"
     CDS             16507..16797
                     /locus_tag="PKM27_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08086.1"
                     /translation="MQSKLRYTKHTLEKIEQFLEEAGYSVRYEKGNFQSGSCVLESKK
                     MIIVNRFFETEGRIIALLDIIHSLPLDEAILSPPALEFYLSLKTEIEKSEMV"
     gene            16794..17564
                     /locus_tag="PKM27_12275"
     CDS             16794..17564
                     /locus_tag="PKM27_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR08087.1"
                     /translation="MRLTFLGTGTSQGIPVIGCQCEVCLSADPKDRRLRSSARIEDGE
                     DRLVIDCGPDFRYQMLRSGTIRLQDVLITHEHYDHTAGLDDLRPFSFIHREPVRIYAQ
                     PEVLDNLKSRFAYAFGENKYPGSPEFELLPAHWNKSFEIGALKIQPLEVNHGRLKILG
                     YKIGEQLAYITDANGLPEETLSRILNIQTLVINALHHRPHHSHFTLDETILQIEKIRP
                     ARAWLIHMSHHMGLHEVINAGLPPHIRLAYDGLMLNLS"
     gene            17612..19621
                     /locus_tag="PKM27_12280"
     CDS             17612..19621
                     /locus_tag="PKM27_12280"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNR08088.1"
                     /translation="MNRLQYESSPYLQQHKDNPVDWYPWGREALDKAATEDKPILLSI
                     GYSSCHWCHVMEQESFTDKEVAAYMNEHFVNIKLDREERPDLDKIYMDAVVAITGSGG
                     WPLNCFLTPDLRPFYGGTYFPPRPAHQRPSWTQVLQHIQRIYTQQKAQVLEQAGRLSD
                     YLTNSGNTLLQLKPEQGTEKSTALEICRKMAKTLQARFDLVSGGFGTAPKFPAVMSIQ
                     YLLDYYFYSGEQTYADHAFFSLDRMAQGGIYDQVGGGFARYATDRNWNIPHFEKMLYD
                     NAQLLKLYSTAYKIRPSPLYAQVVEETVQWLVREMKSDSGGYYSAIDADSEHTEGKYY
                     VWSYEELRSVLGGEQFDLLKKVYEIRPEGNWQDLHHPTSSPVNILWVSENMRGDELLF
                     SRELREIKEKLGRVRERRIKPITDTKLLIGWNALLCQGFLEAWDVFQNPLFLEEARGI
                     LAFLARYGTDPGGDYLHQKDSTIPAMLDDIGYQAAAFLQAYRSLGELQLLEQAGRLLQ
                     YALKHYHDPETNTFSYSKATSDLIAPANDWYDNTTPSAAGTMALTLVHYGRIRNAEYE
                     SIGNEMIRKLEPAIVRYPESLSHWASLLLSQSFGWMEIKGADPDPDALREILKTFIPL
                     VILHRDNKSGEGISFCHNFSCERPVGSIREFRLLLKNHYHPDENR"
     gene            19608..20579
                     /locus_tag="PKM27_12285"
     CDS             19608..20579
                     /locus_tag="PKM27_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNR08089.1"
                     /translation="MKIVKGLVGLFSICLPVLLAAQESSQYTLFTAHKYALNPAYAGL
                     ESTLSITGGYRAQWQELNGSPSSRLLHAHLPLYNLKGAGGIKVEQESFGVEKTLRAAI
                     SYNYVYESSIGLFSAGLGLGFIQKSIDGSALRTPEGRYEGGLVVHQDDILFESKLNGL
                     APQLVAGFYFAQDKLELGVSLENFHRPVIKFNRGKSTYQIFPRLNFYGEYLFEISEQF
                     SIIPAFLVKSDLNRMQVDFSAQATFNGNVIAGLGFRGYSQNTIDALSLIGGLRINPQI
                     QISYGLDLSLSPIKDYHQGTHELMLHYTLNKKIGGVIKEPVIFNPRY"
     gene            20786..22024
                     /locus_tag="PKM27_12290"
     CDS             20786..22024
                     /locus_tag="PKM27_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR08090.1"
                     /translation="MKQRMLRGSLSMLFLLVLFACGGNKQSGYLTGVQDRPKWKGINP
                     YGMVYIPSGTLHVGQSDQDIFSSLTQKPKAISISGFYMDDTEITNNEYREFVQYVIDS
                     MAHTLMGDIIENDDGSTSIDWELPIDWTMEELSEMYYQGEDRLGGNELDVRKLVYTYS
                     WYDLRAAALNPKAPRRSFIKKESTAIYPDTLVWIRDFAYSYNDPIARNYFWHPAYDDY
                     PVVGVNWQMANAFSNWRTQMWNTHRPEEVNSEGFRLPTEYEWEYASRGKKELAPYPWG
                     GYYVRNAKGCLLANFKPGRGNYPEDGGQYTVEVHSYFPNEYGLFNMAGNVAEWTITAY
                     FDNANNFTHDMNPDIQYDAKPGDPDVYKRKVIRGGSWKDISYYLQNGTRTYEYQDSTK
                     SYIGFRNVLSFLGRSINDFK"
     gene            22136..22963
                     /locus_tag="PKM27_12295"
     CDS             22136..22963
                     /locus_tag="PKM27_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNR08091.1"
                     /translation="MATWYKTTSFKYLKNLVIGVGASLVMIGALFKIESLPNSSLFLT
                     IGLVTEAILFLVLGLLPPDKDYYWEKLYPGIEDYHSDVPAILPGVGAVDGKKVSRQLN
                     AEAVENSLSGMLNELQGMSRSMSSLKALQEVDFSQTKQQMKSVGDFYSKMNEAFLALN
                     GSVEDAKKTQQELGVLSKNLTAMNGTYTKVNEAFVALQGSAEDARKTQQELALMSKSL
                     GTLNNTYVKMGETFTSLQASADDAKKTQEQLNVLAKNLSSMNRVYGNVLSAMNVKEH"
     gene            23011..24576
                     /gene="gldM"
                     /locus_tag="PKM27_12300"
     CDS             23011..24576
                     /gene="gldM"
                     /locus_tag="PKM27_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HNR08092.1"
                     /translation="MSIPKQPRQLMINLMYLVLTALLALNVSAEIFNAFRVVDDGLRK
                     SNVALDEANNKIPAEVDKLARKDEVNLRKYADRAAPTRNLSKEFTEYVQAIIDTMVNQ
                     TGGYEVIPNTGVTHLKGEKNKDITTHYMVGRNPAANNGKGAELEKKIEETRNKFLEFI
                     DPEDRAGFMPKVSMNVDSAWQNTKKKNWAHFNFYQMPLGATLPILNKLQNDAKSTEAA
                     VLNYLMSKVGGEDIRFDKYQVVSAPKKGYIISGEKYEADLYLSASSSNVGTNMTALVN
                     GSPVPVVDGVAKYSVVPSGVGVKKYSVRFSVVNPVTNETTTAENEFEYEVGERSVTVS
                     AAKMNVLYKGVDNPIEVSAAGVSSNQINVSFEGPGSIRRNADGTYTLNLENSARPGTN
                     GTVVVSAAGNSARKQFRIKAIPDPVPRIGGIRENRVGNGEFKAQLGIAAVLENFDFDA
                     KCDIAGFTITRAARGEDLMEAPNPGSRFAGEGMRIISLAKPGNSYIFSNIRCKCPGDE
                     VNRKLSDIYVLIQ"
     gene            24581..25432
                     /gene="gldN"
                     /locus_tag="PKM27_12305"
     CDS             24581..25432
                     /gene="gldN"
                     /locus_tag="PKM27_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNR08093.1"
                     /translation="MKNLILVLIFFAGSLGAAMAQRPENVITEASEPGDSSIFMDDIV
                     HRRTILDQRVLPYESLREADIPWEKRLWQVIDVREKLNLPFIYPKAPLFGILNDAATQ
                     GLVAAFRDEDFKQLMQPLEIEGMMFSMDTIQVIDPETYETKTQVVKNEISAATIKFFR
                     IKEVWFFDREASRMKVRILGIAPVRERIIAGKAEEQPMYWLYYPQLREILARHRAFND
                     FNDASPLSWDDIFELRRFSSYVFKESNAQDLRLKDFFPEDGVARLREGEKIKNKLFNF
                     EQDLWEN"
BASE COUNT         6516 a         6523 c         6521 g         5979 t
ORIGIN      
        1 cgatggctac cagtccttcg attccggctt ttttaagctg attgtaagct cttcttctac
       61 cggaaggggt ccggaaggtt tcgctccttg cagactgcag gatggtgccc cccagctgca
      121 tgatgttgct cacatccctg gaagtcattt ggtacatgtc tccacggatc atgccggaat
      181 atccacggtt gatgccaaat acttccattt tatagtgcag ggcggtgcgc actaccgccc
      241 ggatgcaggc attcatgccg ggggcatccc caccagaagt aaaaactcct attcttttca
      301 ttttctataa atatcttctg cgaataagtc ggatgaattc cacggctttc tccctgcggt
      361 cgtcgtggtc ccatttgtaa tggatggccg gcccgggtcc gaggaattcg cgggcttcct
      421 cataagactt aaattggttg aggtctgtga tgatctggtc aaacaactgg cgttgtcctc
      481 caaacagggt ttgtatgatt tccatccggt cggctattcc gattgcacgg ttgatgtccc
      541 tcaggggcag ttcgttgagt ttatcggaaa gttcagatcc tcttcggact tcaaaaagtt
      601 cgaggatgga ggcgggcatg ggattcggtt ttgcttgtac cggttccggt actggtgctg
      661 gttccgatgg ggccggtgcc ggtgctgatg ccggtgccgg gttcgtttcg accggctcgt
      721 tgaccggttt aattgcctcg accacggcgg cctccaccgg tatgggttcc ggctgggggg
      781 gaggaggggg aatgatcttt tccgcaggtt tttcaaccgc cggctcctct atgggttcaa
      841 atttgaattc aagcgtcttt tgtcccaatt ctttttccgg gacggtcggt atagcttcct
      901 gcttgggttt gaaaaccggc agggattgcg gccggatatc gtttaccagt tcgtacaatt
      961 cacgcaaata gttaaggatc aggtcccgtt ccaaagcact taagtcggga ttggcttcca
     1021 gggtatttat catcgcctgc agccttgcca tgattgattt tgccttgatc aaatccatat
     1081 tttacatcaa cggaggggca aatttatgga ttttggcaat aacctcgttt gggaagcgca
     1141 gggcggcttc ctgtacagga tgtcataata aatgggtttt gtgatttcca aaaaaggaga
     1201 aaaggagaaa aggaggaagg gaggaaggaa cctcaacctt agcctcaacc tcaacctcaa
     1261 cctcaacctc aaccttaacc tcaaccttaa ccttaacctc aaccttaccc tccacctcca
     1321 cctatttttt aactttgccc tccatgtttt tggaacccca ttttaaaggt cagcggagcg
     1381 gctggatcga agtgatctgc gggtccatgt tttccggaaa aacggaggaa ctgatccggc
     1441 ggatccgcag ggccaagatc gccaatcaaa aggtggtgat tttcaaacct tcgtccgatg
     1501 tccggtacgc ggagagccag gtggtatccc atgatcagaa taccatcgaa tcggtcccga
     1561 tacgccattc gaaggaaatc ctcaaacttt cggcgggcgc ggaagtgatc ggcatcgatg
     1621 aagcgcagtt ttttgacctg gatctgccgg aggtttgtca gcagatcgcg ttgcggggga
     1681 tccgggtcat cgcggccggg ctggatatgg attatctcgg gaggcctttt ggccccgttc
     1741 caaacctttt ggccattgcg gaatacatca ccaaggtgca tgccatctgc ccccattgcg
     1801 gcaacctggc aacccattcc taccgcctca cggaagagaa ggatacggtc gtcctgggcg
     1861 aaaaggacaa atatgaaccc cgctgcagga cctgttacca tatggggaat atcttaaatt
     1921 ttcagcggga tcgatgatca accggagcca agtaccagta tttcctaact ttgcagctca
     1981 acctgagtca gcatgtcaaa aatcagaatc cgttcagccc ttgtttcggt ttatcataaa
     2041 aacggcctgg acgatattct tcacctcctg gaaaaaatgg aggtcaccat ttattcaaca
     2101 ggcggtacct atcagtttat ccagggcctg ggcatcccgg tggtgagcgt agagcaactg
     2161 acgggctttg ccgaaatcct ggacggccgg gtgaaaaccc tccaccccaa ggttttcgga
     2221 ggcatcctgg cccggcgcga tgaaggccat ctcgcccagc ttgaagccca ggaaatccct
     2281 gaaatcgacc tggtgatcgt ggacctttac ccctttgaaa aaaccgttca gaataccact
     2341 gtagaatccg agatcattga aaaaatagat atcggcggta tttcattgat ccgggccgcg
     2401 gcaaaaaatt acaaagacgt gctcatcgtg ccctcccagg aggattacgc ttttttgcat
     2461 gaactgctgg tcaccaaaga aggatacagc gatctggccg accgcaaatt cctggccacc
     2521 cgggcattcc aggtgtcctc gcattacgat acccagattt tcaggcattt caaccgggat
     2581 gaaaaagtcc agaccctggt tttttccgct cccctggacc ggacgcttcg ttatggcgag
     2641 aacccgcacc aggcagccgg tttttatggc catttggacc gcctgctgca agccctgcag
     2701 ggaaaggaat tgtcctacaa caacctggtc gatgtggacg ccgccatctg gctgatgaaa
     2761 gatatggatg aatttggaag cagtgtggcc atactcaaac ataccaatgt ctgcggcatg
     2821 gcctcccggg ataccctggt gcaggcctgg acagacgccc tggccggcga tccggtctct
     2881 gctttcggcg gggtgatcat caccaaccgc accgtggacg aagaaaccgc atcagccatc
     2941 gaccatttgt tttatgaagt gcttatagcc ccggattttg attttgccgc gctccgcctg
     3001 ctgcaaaaaa agaaaaaccg gatcctgctc aaattgaacc aatggccgga ggaaaagtgg
     3061 atggttaaaa gcctgctcaa tggcctgatc cggcaggaaa gggacctggc gctcaccacc
     3121 aaagcccaac taaaatctgt gaccgtccat acggccaatg aacaggaact caccgacctg
     3181 gtctatgcca accggctggt caaacacctc aaatccaatg ccatcaccct ggtcaaaaac
     3241 aaacaactga tcggcatggg ctgcgggcag acgtcccggg tggacgccct gaagcaggcg
     3301 atcgggaagg cccgccattt tggattttct gacctgctcg ccggatcagc catggcttct
     3361 gaagcatttt tccccttccc cgattgtgtg gaaatcgcgc atgaagcggg tatccgcaac
     3421 gtggtacaac ctggaggttc cgtcaaggac caggaaagca ttgatttttg ccagactcat
     3481 ggtatggcga tgtatctgac cggaatcagg catttcaaac actgatcatg gaccttttcc
     3541 tgtgcctgga tgccgggaac agccgccaga aactggccgt atttcacggg gagcgcatga
     3601 tgtattttca ggtggaaaaa gaattcagca ttgaacaatt aacaaaagta ttggaccggt
     3661 ttcctgtttc ggcggccatg ctttgcgagg ttaaatccct taataaacct attaaaaatt
     3721 tcttaaggaa aaaagtcagg ctgcttgaaa tgtcgcaccg gctaaggctt cctgtgagga
     3781 atgagtaccg gacgccggag acgcttggaa aggaccggtt ggcagcggcg gcaggtgcgg
     3841 tgcgtttata tccgggcgag gcggtgctga tcatcgggct gggtacctgc ataacatacg
     3901 atttcgtttc gggccgcggg gtgtaccgtg gcggggctat ttcccccggt ttggacatga
     3961 ggttccgtgc catgcatgat tttaccgaaa aactccccct ggtaaaattc gacccaaagg
     4021 cggcgctgct cggcaacagc acgaccactg ccttgcaagc cggagcagtt tgggggatga
     4081 cccttgaatt gcagggcatg atcgaacgat atcgcaaaaa atacaaaaac cttaaagtga
     4141 tcctggcagg tggaggcgca gattttttca aaaaaaggct taaaaaaccg atatttgccc
     4201 acccaaattt ggttttgctg ggcttaaacg aggtattatc tttaaatgta ttggaaactt
     4261 agattacctg gagtcttatt attggtgctc tcattctgcc ggttgacggc ccaggaaaag
     4321 ccaagggaat attctcccta ttccaggttt gggttcggag atcttaccga accccatttt
     4381 tccgcgctgg ggcagctggc tggcatcgcc gccggatttc atgaccggaa catgctcaat
     4441 tacagcaacc ccgcggccct gggttccctg cgaaatactg catttgaagg gggggtcagc
     4501 accaaattca cgaccctgaa aaaccaggat ttgagtaaag atttttttgg cggagggctg
     4561 gattacctgg ccctgggggt cccgatcttc aacccgatca acgaggccct ggaccgcctc
     4621 gaacgaaaat tccactgggg gatgttggtg gcgcttaagc cattcagcca cgtgggctat
     4681 gatgtgtccc aaatcgacag catcaccggt accggccggt atgaaaggcg ctacaccggc
     4741 agcgggggca cttatgagtt taagtggagc aatggatttg aataccgcaa tttcagtgcc
     4801 ggcctgggcc tcggctacct gtttggcagg atgtcctacc ggcgcaacct cgatctcctg
     4861 gccgtttccc tgccttacag cacgattttc ctggatgaaa tttcggtgaa aggattccgg
     4921 tggaatgcag ggcttcagta caggataaac ctcaataaac cggtggaggg agaaagcgaa
     4981 tccaccaaga cgcttacctt cggattatat ggccagacca gccaatcgta taacaccatc
     5041 agtgaccatt tgtaccgccg ccagctcatt gtgagcgcgc aggtgggcgt cgtagatacc
     5101 gtccgggacg aaaaagaaat caaaggccgg ggaaaactgc ccggagaatt ccacctgggg
     5161 gttttctaca gcgaccggta taaattccag gccggtggcc agctcagcct ggtttcctgg
     5221 tcggcctata ccaatgacgc caagccggaa atcctggaca attcctccca gtttgccttc
     5281 ggcttgtcct ggtgtccgga tatatcctcg cttgacaacc tgttaaaaag gataacttac
     5341 cgggtcggtt tcaaggccgg gaatgacccg agaagttttg acggggaaca attgaaaaat
     5401 tacggtgtaa ctttcggagt tggcgttccg tttgtatttc aaagaaaagt ttcttttgct
     5461 aatttcgccg tcgaaacggg gagcatgggt ttgaaggaca agttgaaaag taactatctt
     5521 caactcaaac tcgggttcac gctgaacgat gacgaatggt ttttgaaaag aaaatacaat
     5581 taacctttaa actgtttaat cactcttaaa aatgaaaaaa tcactgtaca caatcgccct
     5641 ggtctggttg gtttcttcat ctttgtcctc atccctgaat gcccagtgca acacctggtt
     5701 gaattccccc caaaaagacg aggcggagac tgcccatacc ctgtaccggg atgccatcaa
     5761 agcgggaaaa ataatggaag ccatacccaa ctgggagaag gcctttaaca ttgccccggc
     5821 tgccgatgga ctcagggact ggcatttcag tgatgggatc acgatttata aagccattta
     5881 cgaaaaagag acggatgcgg ccaaaaaaga ggcagcactt aaaaaagtga tggaattgta
     5941 tgccggcatg atcgaatgtt ataaagccaa atcgataaag atccccaact gcaacgacga
     6001 ggcatgcctt caaaagaaaa tcggggacat caaggccctt caggcctacg atatgtatta
     6061 cagccttcgc aacccgtatc cggaggtgct gaaagtgctt gcggaatgca ccaaaatcag
     6121 cggtaaggat gccctctata cgatctttgt accttatgcc gacatcgccg tttaccagtt
     6181 tctcgacaag gccctgccca aggaagaagc ccgggcgatc catcagaaac tgaatgaaat
     6241 cggcgaacat aacgtggcca ccaataagaa atacggttcc tattataaag atgccctggc
     6301 ttatgccaac gccaaattcg ccgaaatcga ggattttata ttcgactgcg attatttcaa
     6361 ggaaaagctg gaaccggaat accggaagga tgccgaaaat catgaaacca taaaaatggt
     6421 attcaataaa ctttcatcca aaggctgtac ggaaaccgat ccgctcatga ttgaactcaa
     6481 aaccaaattt gaaaaaatcg tggcggaaga aaatgcccgc aggagggccg aatttgaagc
     6541 cagcaacccc atctatatgg ctaaaaaaat gatcgacgaa ggcgattttg acggcgccat
     6601 cgccaaatac aaagaagcca tagagtccgc ggagacggat gatgagaaag gggaactggt
     6661 tttgtccatt gcctccgttc agttccggaa gctgggtcag gccgccgctg cccgggaatc
     6721 cgccagaaag gctgcgggcc tcaaggacaa ctggggagcc ccttacctgc tgatcgggga
     6781 tatgtatgtc tccggggcgc gcaattgtga tgacgactgg ggttcaaggc ttgcggtcct
     6841 ggcggcgatc gacaaatatc agacggccaa gagtattgat ccgtccatga cttcggaggc
     6901 caatgagcgc atcggccgga tctcttccgc aatgcctgca aaagacgatg gatttatgcg
     6961 tggtcataaa gaaggccagg tgctcaccgt accctgctgg atcggggaat ccgtccggtt
     7021 gagatttaaa gactgatcgc ggtaaacacc aaaaaatata cagagcctca tctttaccgg
     7081 tgaggctttt ttatttttat gtcccttttt gggcttaact taagctaatg atgttgaaaa
     7141 atacctttcc ggtcctgttg ctcgcaatcc tgtcctgttc acccaaaaca caactgcccg
     7201 cgcctccgcc ggcggccccg caaccggcat cccccgcggt ggatttgagc aatctcagcc
     7261 cctgccccaa ctggctaagc ctgccaaacc aggacgatat caccaatgaa tatatcatat
     7321 accgggacgc cctcaaggca ggcaattact cccgtgcttt cgagggatgg aagacggtgt
     7381 atgccgtggc gccggccgct gacggcaaga ggtccacggt cctggacgac ggcatccgga
     7441 tctataccca tctgttcaac caggaaaagg acgaaaccaa aaggaaagaa tacgtacaaa
     7501 aagtgctgga cctgtatgat cagatcggga cctgctaccc gaccggcgcc aatgtggatg
     7561 cacagaaagc atttcagtat tattattcct tcccgggata cgctacggat gagcagattt
     7621 tcaccctctt taaaaaagcc ctggaccagg atggtttgaa agcggactat tttatcctca
     7681 acccgtttac cgccctgctc atccagcgtt ttgtcgacaa taagatcacc ctggaagaga
     7741 cgcaaaaata tgccgggctg gtaagatcca ttctccagga aggattgaaa aactgcaaga
     7801 cccctgcagc ctgtgagccc tggaacatta tcaacgaata tgtaccggca cgcctggaag
     7861 acctggaagg agtggaagga ttttacgact gtacctatta taaaaacaaa tacttcaggg
     7921 atttccaggc agcccccacc gactgtgaag ccatcaatac cgtctatggc cgtttacgct
     7981 gggccaaatg cccggatacg gacgaagcag tcctggcctt gagggccgca cgggatcaaa
     8041 actgcagggt ggtcgtcgaa gtaaaagaaa cgaccctgag taaagccatc gacgccctca
     8101 acgaaggaaa ttaccgccag tcggtggaat tgctgaagca ggccatcgag gtgaccgaag
     8161 atcccaaacg taaggccgaa ctggccatga ccatttccaa agtctattac ggaaaccttc
     8221 gaaatttccc cgaatcccgg agatgggccc tgcaggcggc cgaatacagg ccaaactggg
     8281 gagagcctta cctcaccatc ggcaaacttt atgcatccag cgggccttta tgcggccctg
     8341 gtaggggtta cgacagccaa caggtcgtct ggccggccat cgacaaatgg aattatgcca
     8401 agtccattga tccaaccaca gcagcagaag ccaataaact gatcaaccaa taccttcaat
     8461 atatgcccac cagggaagac ctccatatgc gcagcatttc ggaaggggaa aacatccgga
     8521 ttggatgctg gataaatgaa accaccaaaa ccaggaccat caagtagggt tcagagcaat
     8581 ttattgacct ggttccggac ttcctggata gaagggacac cggcaaccct ccagcccaac
     8641 ttgcctgatt taaagatcat gacggtgggt acggcctgca cctggtattt ttccgtcagg
     8701 ctcctgtttt tgtcgatgtc gattttatag attttaatct ggtcgtcgta ctcgtccttg
     8761 atttgttcca gcatgggggc catcatttta cagggtccgc accagtcggc ataaaagtca
     8821 atgatgaccg gtttttcctg attgatcagt tgtgaaaatt gcataacaat atgtcaacgt
     8881 attctcctgg tccggggttc tcttaataaa tggagatttt tgcggaaata ttaaaaacaa
     8941 tgaataaatc aatttttttt ctccttctga tggttttggc tatagcctgc caaagtaaaa
     9001 agacggatac ccccgccgaa gaaacgcagg tgaccgaagc cagccccgca gaggccaccg
     9061 aaaccgcccc ggcggcggcc tccctttccc ctgaaatctg gaattccgaa ctgggtgccc
     9121 ggttaaaagc cgattataaa gaaaacccgg ccaaccaggc cgaaaccgac cagaacctca
     9181 ttctggaata tgcggtgcag aacaatcttg atgtgaagcg gaccccgtcc ggcctttatt
     9241 atatcatgga aaaagaaggc gttgggaaat cacctaaaag tgaaaatgtg gtttccgtaa
     9301 attatcgcgg ttattttctc gatggaaagg aatttgattc cagctacaaa cggaatgccc
     9361 cgatccagtt gcccgccaac cagtttgttc ccggctggac agaagccctg acgatgatga
     9421 aacccggagg caaaatgaaa gtcctgatcc cctcccacct ggcttatggc cccgtcggat
     9481 accccggcgc catacctggc aatgcggtcc tgggatttga tatggaattg cttaaagtgg
     9541 tgcaataaaa aaaagtgaaa aaagtgaggg gttgactcga agtcaacccc tcactttttt
     9601 tactttttaa gcatattggg cgatgatctt ctcggccagt tctttaccga tagcggcctg
     9661 ggcttcgcgg gtagagccgc cgatatgagg agtgacggag attttcggat gcctcaggat
     9721 ttcaatactg gggttgggtt cattcaggaa agcgtccaga cccgctccgc ccaattgacc
     9781 gctctccagg gcctccagca gggccgtttc gtccacggta cccccccgcg ccgtattgat
     9841 gacacaggct ccctttttca ttttaccgaa ttcttccctg cccagaagag gtttctgact
     9901 ggagggcaca tgcagggaaa caaagtctga ccggatcagc aggttttcaa gggtgtcggt
     9961 gggaacggaa agggagaacg aatggcttcc gatttcgaat ttcaactccg cggtctggat
    10021 catcgggtcg gcggcgatca ctttcatgcc gagtcccagg gctattttgg ccaattcctg
    10081 gccgatccgc ccaaatccaa tgatacccag ggtttttccg agcaactggc tcccggaaga
    10141 ataattcttt ttcagggcgt tgaaatcggc gccctccgcc cgcatctgga aattggattg
    10201 ttgcagcatg cgggccaggg caaacatatg cccgatggcc agttctgcca cagctcttga
    10261 ggaagctttt ggcgtgttga tcaccgtgac attcctggac cgggcataat ccacatcgat
    10321 attgtccatg ccgacccctc cgcgcgctat gatcctgaga tgggggcagg cgtcgatcag
    10381 gtctttacgg actttggtgg cgctgcgcac gacgatgacc tcgtattgtt gcagcgcttt
    10441 ggccaggtcg gcctgctcca ctttattagt gtctacggta aatccggcga cttccagggc
    10501 ggttttgcca tcggcctcga tgccatcgtt ggccagtata cgtttataca ttttcctttt
    10561 gttagatccg caaataaaga gctatttgag gaaaaaaaga aaatatattt cggttgattc
    10621 tacggggttt aaccctaaag cggcagagcg ggggatccct gtcttatttc aggagagccg
    10681 gtatcaccac cattccccaa ggcaaggttt acttattggg cgtttttttg gcagcctcct
    10741 catactcctt cagtttttgt ttcagttcca ggttttcttt gatcaggctt tcgatctgcc
    10801 ggtctttgat ctcaagggaa ttttccaaat ccgactgttt tattttttcc ccttcccttg
    10861 ctccaggact ttcaacgggc agggaggtgg tcgtttcgga ggactttttg gaatcggagg
    10921 aactaaagac cgacaggcct tttttaaaaa ggttcacaat gtttccaatg ccatcctgtc
    10981 cgtggtagta ggatgccccg atataggcca aaggggccag gataatgaga aaaagaaaaa
    11041 aacgaaaaca tccggtcgtt ttatagggtc tgggcataat tcatttcgtt tataaaattc
    11101 aaaattaaag cccaatcaaa gggagccaaa caaatatcta tgaaaccggc gataaatccg
    11161 acagaaccgg tcgcagcggt tgcactcagg aaattttgct ccagccgggg gaccgcaatt
    11221 cctcctggtg caggtattcc agcaacagtt tgttgctggg atggatcagt tcaggatccc
    11281 gttcaatgat tttcgcgacc atcttctgca cctggtccag gatctgcaga tcggtgacca
    11341 ggttgaccat cacaaaatcc agtcccccgc tctgacgcgt gccttccagc tcgcccgggc
    11401 ccctcagttc caggtccact tccgcgatcc ggaaaccatc ggtggtctcg cacatggtct
    11461 tgaggcggat cttcgcttct ttggataatt tgtaaccggt catcaacagg caataggatt
    11521 gatctgcccc ccggccaacc ctgccccgca gctggtgcaa ttgggatagg ccaaaccgtt
    11581 cggcgttttc aatcaccatc acgctggcat tggggacgtc cacgcccacc tcgatcacgg
    11641 tggtggctac caggatatgg gtcttgcccc ggctgaactg ctgcatttca aaatccttgt
    11701 cttcgggccg cattcgcccg tgcagcacac tgatctggta ggccggcctg ggaaaatgat
    11761 gcaataattg ttcatacccg ttttgcaggt cctgcagatc cagtttttcc gattcttcga
    11821 tcaatggata gatgatatag atctgcctgc cccgggcgat ctggtctttg aggaactggt
    11881 acacctgggg ccggctcttt tcattatagt gcatggtttt gaccggcttc cttccgggtg
    11941 gcaactgatc aatgatggat acgtccaggt ccccgtaata aaccatgctc aacgtccgcg
    12001 ggatcggggt ggcggtcatc accaggatgt gagggcttat tttttcgttt ttttgccata
    12061 ggttggcccg ctgggcgacc ccaaaccggt gctgttcatc aatgatggcc agccccaggt
    12121 tgtggaaacg caccgggtcc tccagcaggg catgggtgcc gatcaggatc ttgatatccc
    12181 cctggcgaac cagccgcagc agttccttcc ttttttcgcc tttgacactg ccggtgagca
    12241 ggcccaccgg caaaccaagc cctttgagcg cgtaggtgat ggacgcatag tgctgggtgg
    12301 ccaggatttc ggtaggggcc atgagacagg cctggtattc attgtcgatg gccagcagca
    12361 tgatcataag gccgacgatg gtcttgccgg cccctacatc cccctggagc aatcggttca
    12421 tctgtgcgcc cgaggccgta tcgtggcgga tttctttgat gacccttttc tgggcttcgg
    12481 tcagctcaaa ccggagatga ttgcggaaaa actcattaaa actggtccct accttttcaa
    12541 acacagggcc tttcagatgt ttcttccgga aggatttggt ggtcagtaat ttcaattgct
    12601 ggaaaaacag ctcttcaaat tttagccgtt gggtagcccg ttccagttcg ttgcggcttg
    12661 aagggatatg gatccagcgg atcgcctccg cccggctgca caggttcatg gaacggacca
    12721 ggtaatcagg cagggtttcg gggagtgcgc cggggggcag gttttctatc agggtggtga
    12781 tcagtttcct gcgcatgcgc gaatcgagcc cctttttatt gagtttttcc gtgctgttgt
    12841 agaccggttc gaagccgggc atgcggacca gttcactcaa aagatccagt tccggatgag
    12901 ccatggtaaa ggggtaaccg tatccggtca ggtttccaaa tgccaggtat tcctggccgg
    12961 gcctgagact ttgttccagc cattgggagc cctggaacca aatcaggtta agcaccccgc
    13021 tctggtcctg gatgctcgct ttgatccttt tttgaaattt agtacctgaa aaactcaggg
    13081 cggacaggat cccccttacc tgaaccgcct gaccgtcgtt gcggatttga cggatggggg
    13141 tgatggcaga acggtccaca tgccggaatg gaaaggcata gagaaggtcg gcaaaggtga
    13201 agatcttcaa ttccgtcttg aacaaatccg ccttcacagg acccacccct ttgagatatt
    13261 caatcggact cttcagataa tcgggcctga ccagcacttt ttgccttaaa aatacgggta
    13321 gaagctggga gttggaagct cgaagctagg ggctcgaagc tggtggccgg aagccaccga
    13381 aatcccggga gtctacagaa atcccgggcc aaaacgcccc taacctccag cttctaactt
    13441 caagcttcaa gcttccacct cacatccccg ctatccttat ctttgcacct ggtactgaag
    13501 ccaagcataa atcctcctga ctatggaaag caaaagacaa caacaggtag cggaactcat
    13561 attgcgtaat ttttcctatg ttctccagca agagggattg tatatctacg gcagagaacc
    13621 cctggtgacc gtgacttcgg taaaggtggc ctcggacctc caactggcca ggatttacct
    13681 gagtatttac aatacggaaa ataaggaggc catcctgggg cagatccggg agcacgatca
    13741 caaactcaag caggagatgt accaccggct gaaaaaacaa ttaaggcgta tgcccgcttt
    13801 agaatatttt attgacgaga ccctggatga aatgtaccgg gtcgaagcct tgttccaacg
    13861 gctggaaggg caaaagcagg ctccaggtac cggggccggg gaataatcag ccttttttgg
    13921 gcggaatcgg tgaaccatgc gggtctatgg aaggataacc cagtttggca tcgatttcat
    13981 ccagcaagtc atcgggactg tggtgctcct ggtattccgc ttcgtcatgg atctggtctt
    14041 cggtcagccc gatttggttg acgagatagg tttcccagag tcggtgggca cggaccaggg
    14101 cctgcgcccg ggagatccct tccggggtga ggctgaggtc cggagtcagt ataatctggt
    14161 tacgccggtt gagataacca agtaattttt tcaggcgggg cgcaggaatg gagattttgg
    14221 tgatcaatgt ttttaaacca agttccccgg cttcctgcag tttaaaggat tccttgagga
    14281 tatcttccag gtccactttg agtttttgcc tcctctgtct ttgccacctt ttgatcaatc
    14341 ctttccgggg tgcgaaaaaa acagccagga cataaatcaa ggtggccatg acagccatgg
    14401 ccggtccggg agtggtctga gcatagatgg acaggaaaag gcccagcacg gcggagacaa
    14461 ggccaatgag cccggacaga accaggaccc ggtgaaggcg gtccgacaac agcagggccg
    14521 tggaggccgg ggtgatgagc atggcgacca ccaggatcac ccctaccgtc tgcagggcgg
    14581 ataccaccac gatggacagg aggatcatca gggcatagtg gaccgatttg acccggatgc
    14641 cctgggtgaa cgcaatggtc ggctggaaag tggtgatgaa caagggccgg aacaaaaaga
    14701 ccaatcccag gatcaccacg accgaaatca acacggtgag ttgcaggtcg gagtcctgga
    14761 tgcccaggat atttccaaaa agaaagtcct tcagatcgag gtgaaaaccc ggcgtatggg
    14821 ataaataaga gatccccatg actccgatgg aaaacatggc ggtaaaaacg atcccgatca
    14881 cggcatcgtt tttcgtcttc gcgttttgct gaaaccaggt catcagcacg acggtaacaa
    14941 tcccggctgc cagggaacca ataaataccc ccagcaagtt ttgacctacc aggacataag
    15001 ccaggaccac ccccggcaaa atggagtggg agagggcatc cccgatcagg ctcatatttc
    15061 ttaatacaat gaaacaaccc aaaaggccgc aagtgatgcc taccaggctt ccggcaagaa
    15121 gggccctcat ggcccaggcc gattgtaaga tctcgacgat atccatattt aggcaaatat
    15181 attaataata tttagacttg cctaaatata tttttaaaga tatccttgta atttcattta
    15241 ggtttataat tggtaaccag tgtttcaaaa aatttaagcc ccggttcgga gatcaggttg
    15301 gtccctaccg gttttttatt ttctataatg gtgaagtacc cgcaaccggt tcccaaaaag
    15361 tgaatgtctg cctcccggat gatcttgccc ccggattgaa aggatattct gccaatggca
    15421 gggcaattca tgccctccgg tttggcttga ttgtcaaaga aattgacggt ctgggaaatg
    15481 gaggctttgt cactctggct gacgctcaca tccatattga aaaacaggaa gtccacaaaa
    15541 tcagcctgtt ccgacagggc cataatttgc tgaaggttta aataagccat ggattcaggg
    15601 gggactttgg gttttgaagg accggaattg caggaaacca gcagtccgag gcagatccag
    15661 ccatggataa tcttcatatt cccaaatgta gtcagaaatg gtaaaaaaag tcgcaggaga
    15721 tgaaataccc cgtcatggaa taaaccctcc tttcattaat ggttgggaca aggtcggaat
    15781 ccagcaactt gttcatggag cggttgaacc ggaaactgag gcctgcctgc tcgtccactt
    15841 taacattgat ccccaaaatc aacgaaaagt cagtggtgtt cagaaagggg ggcaaatggc
    15901 tcaggggaat ctgccgctgg tcgcggtccc ccgccggatc atacacattg gaccggatga
    15961 ggcggccggt gcctacgcct gcgcaagcct ggatccggta atactccttg atcgggtgga
    16021 accaatcgtt gaggaccacc agggcattga tggatgcata gtccaggatc atcttcctcc
    16081 ccttgtcgtt ggactgggaa aagcctttcg attggctgcc ccgctggttg taggcgagtt
    16141 cggtatgaaa ttccagtttg ggaatgatgt aagcgatacc ccggagtccg gcgttgatcc
    16201 ccattttgtc gtaaccctgc atgtcgtccc cgtcgatctg ggaaagattc agcccggcaa
    16261 aaatccccga accgaaacgg agggcctggg cattggatcc cgatggaatt cccgaggtaa
    16321 tcaggaataa aaagaaaccc aggcggtaca tattaaaaaa aattatatga aaattacaat
    16381 tccccgaatg aaatgatgac ttttgtcaat agtattaaaa acgtttaaat cctccgggtt
    16441 gttaatgcca gccatttatc caattcacgc ggctgaataa acttcccgga cctgtcctat
    16501 aagcctatgc aatcaaaatt aagatatacc aagcacacgc tggaaaagat cgaacaattc
    16561 ctggaagaag ccggttattc cgtaagatac gaaaaaggca atttccaatc cgggtcctgt
    16621 gtcctggaaa gtaaaaaaat gatcatcgtc aaccggtttt ttgaaacgga aggcaggatc
    16681 atcgccctct tggacatcat tcattcgttg cccctggatg aagccatcct gagtcccccg
    16741 gccctcgaat tctacctgag cctcaaaacg gaaatcgaaa aatcggagat ggtgtgaggc
    16801 tgaccttcct tggaacaggc acttcccagg gtatcccggt gatcggctgc caatgcgaag
    16861 tctgcctgtc cgcggatccc aaagaccgaa ggctcagatc ctcggcaagg attgaggatg
    16921 gtgaagaccg gctggtcatt gactgcgggc cggatttcag gtaccagatg ctgcgttccg
    16981 ggaccatccg gcttcaggac gtactcatca cccacgaaca ttatgaccat acggccggac
    17041 tggatgacct gaggcccttt tcttttattc accgcgaacc ggtgaggatc tatgcccagc
    17101 ccgaagtgct cgataacctc aaaagccggt ttgcctacgc tttcggcgaa aacaaatatc
    17161 ccgggtcccc ggaatttgaa cttcttccgg cccattggaa taaatctttt gaaatcgggg
    17221 ctttaaaaat ccagcccctg gaggtcaacc acggcaggtt gaagatcctc ggttataaga
    17281 tcggggaaca gctggcatac atcacggatg ccaatggcct tcccgaggag accctgtccc
    17341 ggattctgaa catccaaacc ctggtcatca acgctttgca ccaccggccg caccattccc
    17401 atttcaccct ggacgagacc atcctccaga tcgaaaaaat ccggccggcc cgtgcctggc
    17461 tgattcatat gagccatcac atgggcttgc acgaggtcat caacgcaggt ttgcccccgc
    17521 atatccgcct cgcttatgac gggttgatgc tgaatctttc ctgacctggc ctgtagtccg
    17581 ggcggaaatc ctctactttt gtccgtccgg tatgaataga cttcaatacg aatccagccc
    17641 ctacctgcaa cagcataagg ataatcccgt ggactggtat ccctggggca gggaggccct
    17701 ggacaaagcc gcaaccgaag acaaacccat tctcctgagc ataggataca gcagttgcca
    17761 ctggtgccat gtcatggaac aggagtcctt taccgataag gaggttgctg cctacatgaa
    17821 tgaacacttt gtaaacatca aactggaccg tgaagaaaga cccgacctgg acaagattta
    17881 tatggatgcc gtggtcgcca tcaccggctc cggcggatgg ccgctgaatt gttttctcac
    17941 cccggacctg cgtcctttct acgggggcac ctatttcccg ccaaggcccg ctcatcaaag
    18001 gccctcctgg acccaggtat tgcaacatat ccagcggatc tatacccagc aaaaggccca
    18061 ggtcctggag caggccggac ggctttccga ttatctcacg aattccggaa ataccctctt
    18121 gcagctcaaa ccagaacagg gaacggaaaa gagcacggcc ctggagatct gccggaaaat
    18181 ggccaagacc ctccaggcaa ggtttgatct ggtttccgga ggatttggta cggctcccaa
    18241 atttcctgcc gtcatgagta tccagtacct cctggattat tatttctatt ccggagaaca
    18301 aacttatgcc gaccatgcct ttttttccct tgaccggatg gcccaggggg ggatctatga
    18361 ccaggttggc ggcggttttg cacgctatgc cacggaccgc aactggaata ttcctcattt
    18421 tgaaaaaatg ctctacgaca atgcccaatt gctgaagttg tacagtacgg cttataaaat
    18481 ccggccgtcc cccttatacg ctcaggtagt ggaagaaacc gtacaatggc tggtccgcga
    18541 aatgaaatcg gattcgggcg gatattacag cgccatcgat gcggacagcg agcatacgga
    18601 aggcaagtat tatgtttgga gctatgagga actgaggtcc gtgctcggtg gcgagcagtt
    18661 tgacctgcta aaaaaggtct acgaaatacg accggaagga aattggcaag acctgcacca
    18721 cccgacgtct tccccggtga atatcctgtg ggtttcagag aatatgaggg gggatgaact
    18781 tttattttcc agggagctca gagaaattaa agaaaagctt ggccgggtca gggaaaggcg
    18841 gattaagccc attacggata ccaaattgct gatcggttgg aatgctctct tgtgccaggg
    18901 tttcctggaa gcctgggatg tttttcaaaa tccgctgttc cttgaggagg ccagggggat
    18961 cctggctttt ttagcaaggt atggaacgga cccgggcggg gattacctcc atcaaaagga
    19021 cagcacgatt cccgccatgc tcgatgacat cggttaccag gcggcggcct ttttacaggc
    19081 ctaccgcagc ctgggcgaac tacaattgct ggaacaagcc ggccggttgt tgcagtatgc
    19141 cctgaagcat tatcacgacc ctgagacaaa tactttttct tattcgaagg ccacctccga
    19201 cctgattgct ccggccaatg actggtatga caatactacg ccctctgccg ccggcaccat
    19261 ggccctgacc ctggtgcatt acggaaggat ccggaatgcg gaatatgaat cgatcggcaa
    19321 cgaaatgatc cggaagcttg agcctgccat cgtccggtac ccggagtccc tgagccactg
    19381 ggccagtctt ttgctcagtc aatctttcgg atggatggaa atcaaagggg ctgatccgga
    19441 cccggatgct ctccgggaaa tcctgaagac ctttatcccc ctggtcatcc tgcaccggga
    19501 caataagagc ggcgaaggca tcagtttttg ccataatttt tcctgtgaac ggcccgtcgg
    19561 atcaattcgt gaatttcgtt tacttttaaa gaatcattat catccggatg aaaatcgtta
    19621 aggggcttgt tggtttgttt tccatttgcc tgcccgtcct gctggcagcc caggaaagca
    19681 gccagtatac cctttttacg gcccataaat atgccctcaa tcccgcctac gcgggactcg
    19741 aatccacgct cagcatcacc gggggatacc gcgcccagtg gcaggaattg aatggcagcc
    19801 cgtccagccg gctcctgcat gcacaccttc ctttgtataa tctgaaaggg gcggggggca
    19861 tcaaagtgga acaagagtcc tttggtgtgg aaaagacttt aagggccgct atttcctata
    19921 attacgttta tgaaagcagt atcggcttgt tttccgccgg gcttggcctg ggttttatcc
    19981 aaaaatccat cgacggcagc gccctgagga cgcccgaagg caggtatgaa ggcggattgg
    20041 tggttcacca ggacgatatc ttgtttgaat cgaaactgaa cgggcttgcc ccgcagctcg
    20101 ttgcaggctt ttattttgcc caggacaaac tggaattggg cgtttccctt gaaaatttcc
    20161 atcggccggt catcaaattc aaccgcggca aatccaccta ccagattttt cccaggctga
    20221 atttttacgg agaatacctt ttcgaaatca gcgagcaatt ttcaattatt ccggccttcc
    20281 tggtcaaatc cgatttgaac cgcatgcagg tggatttttc ggcacaggcc accttcaacg
    20341 ggaacgtcat cgccggttta ggcttcaggg gctattccca aaatacgatc gacgccctct
    20401 ccctgatcgg ggggctccgt ataaatcccc agatccagat ctcctatggc ctggacctct
    20461 ccctttcccc gatcaaagat tatcaccagg gcacccacga actgatgctc cattataccc
    20521 tgaataaaaa gatcgggggg gttatcaagg agccggtgat cttcaatccg cggtactaaa
    20581 gtcctttttt aacgaaagtt taatacagtc gggcatgatt cgtgtaatat gtgccccgga
    20641 cagccgttat aagcttaagg gacagaatct tgtaatcgaa gtaccaaaat gattcgcggg
    20701 gaaaatttta gataccggaa tgatgacaag aaagtatctt tacgcccaaa tttttattga
    20761 gccagtattt ttgaaactta gaaacatgaa acaaagaatg ctaaggggga gtttgagcat
    20821 gctctttctg ttggttttat ttgcctgcgg gggcaacaaa caatccggtt atctgaccgg
    20881 ggtccaggac cgcccgaaat ggaaagggat caatccttac ggtatggttt acatcccctc
    20941 cggcaccctc cacgtcgggc aaagcgatca ggatattttc agttccctca cccaaaaacc
    21001 caaagcaatt tccatttccg gtttttatat ggacgacacc gagatcacca ataacgagta
    21061 ccgggaattt gtgcaatatg tgatcgattc gatggcccat acgctgatgg gcgacatcat
    21121 agagaacgac gacgggtcta ccagcattga ctgggaactg cccatcgact ggaccatgga
    21181 agaattgagc gaaatgtatt accaggggga ggaccggctg ggcggtaacg aactggatgt
    21241 tcgcaaattg gtttatacct acagctggta tgatctgcgt gcagcggcgc tcaatcccaa
    21301 agcacccagg cgttccttca tcaaaaaaga gtccactgcc atttatcccg ataccctggt
    21361 ttggataagg gattttgcat actcctacaa cgatccgatc gcccgcaact atttctggca
    21421 ccctgcctat gacgattacc cggtggtggg ggtcaactgg cagatggcca atgccttcag
    21481 caactggagg acccagatgt ggaataccca ccgcccggaa gaagtaaact cggaaggttt
    21541 ccgccttccc accgaatatg aatgggaata tgcctcccgg ggcaaaaaag aactggctcc
    21601 ctatccctgg gggggctatt atgtaagaaa tgccaaagga tgcctgctgg ccaatttcaa
    21661 gcccggcagg ggcaattatc ccgaggacgg cggccagtac actgtggaag tgcattcgta
    21721 tttccccaac gaatacggct tgttcaatat ggccggcaat gtggccgaat ggaccatcac
    21781 cgcttatttt gacaatgcca acaattttac gcatgacatg aaccctgata ttcagtatga
    21841 cgcaaaaccc ggtgatccgg atgtgtataa acgcaaagtg atccgcggag gatcctggaa
    21901 agatatatcc tattacctgc aaaacgggac gcggacttac gaataccagg actccaccaa
    21961 atcttacatt ggtttcagaa atgtattgtc tttcctgggc agatcgatca atgattttaa
    22021 gtaggacctt ccggagaaag caggatttcc attgcagccc catcagccgg catgatctga
    22081 atcggagacg attccaacac gtatgaattt ataaaatttt aatattaatc ataatatggc
    22141 aacctggtat aaaaccactt ctttcaaata tttaaaaaac ctcgttattg gtgttggcgc
    22201 atccctggtg atgattgggg ccctcttcaa aatagaaagc cttcccaatt caagtttgtt
    22261 tttgaccatc gggctggtca cggaagccat cctcttcctg gtgcttggcc tgctgccgcc
    22321 cgacaaggat tattattggg aaaaactgta cccgggaatt gaagattatc attcggacgt
    22381 gcctgccatc ctccccggcg tgggcgcggt ggacggcaaa aaagtgagcc gtcaactcaa
    22441 tgccgaagcg gtggaaaaca gcttgagcgg aatgcttaat gaattacagg gcatgagccg
    22501 gagcatgtcc tcgctcaaag ccctccagga agtggatttc agccagacca aacaacagat
    22561 gaaatcggtc ggggattttt attccaaaat gaatgaagct ttcctggcgc tcaacggatc
    22621 cgtggaagac gccaaaaaaa cccagcagga actgggtgtg ctttctaaaa acctgactgc
    22681 catgaacggg acctatacca aggtgaacga agcttttgtg gccctccagg gttccgccga
    22741 agacgccagg aaaacccagc aggaactggc cctgatgtca aaaagcttgg gcacgctcaa
    22801 caatacttat gtaaaaatgg gggaaacctt tacctcgctc caggcttccg ccgacgatgc
    22861 caaaaagact caggaacaat tgaatgtact ggctaaaaac ctttcatcca tgaaccgggt
    22921 atacggcaac gtgctgtcag ccatgaatgt aaaagaacat taaatcccaa tcaactttaa
    22981 aatcctttat cgtccatttt aatctgatcc atgtccatac ccaagcaacc ccggcagctg
    23041 atgatcaacc tgatgtacct ggtactcact gccttgctgg cactaaacgt atctgccgaa
    23101 atcttcaatg ctttccgggt ggttgatgac ggccttagaa aaagcaatgt cgccctggat
    23161 gaagcaaata acaaaatacc cgcagaagtg gacaaactgg cccgtaaaga cgaagtgaac
    23221 cttcggaaat acgccgaccg ggccgctcct acccggaacc tgagcaagga attcacggag
    23281 tatgtccagg ccatcatcga tacgatggtc aaccaaacgg gcggctatga agtgattccc
    23341 aataccgggg tgacccatct taaaggagaa aaaaacaaag acatcaccac gcattatatg
    23401 gttggccgga atccggcagc caacaacggt aaaggcgcag agctggagaa aaaaatcgag
    23461 gaaacgagaa ataaattcct ggagtttatc gatccggaag accgggccgg gtttatgcca
    23521 aaggtatcca tgaatgtcga ttccgcctgg cagaatacca aaaagaaaaa ctgggcccat
    23581 ttcaattttt accagatgcc cctgggagca accctgccca tactcaacaa actgcaaaac
    23641 gatgccaaaa gcacggaagc cgccgtactc aattacctga tgagcaaggt agggggcgag
    23701 gatatccggt ttgataaata ccaggtcgtt tccgcaccca aaaaaggcta tatcatttcc
    23761 ggggagaaat acgaggccga tctttacctc agcgcctcct ccagcaacgt cggaaccaat
    23821 atgaccgccc tggtcaacgg gtccccggtg cctgtggtgg acggggtggc caaatattcc
    23881 gtggtgcctt ccggggtggg ggtgaaaaaa tattcggttc gcttttcagt ggtgaacccc
    23941 gtgaccaatg aaaccactac ggcagaaaat gaatttgaat acgaagtcgg tgaaaggtcg
    24001 gtcacggtat ccgccgccaa aatgaatgtg ctttacaaag gagtggacaa cccgatcgaa
    24061 gtctctgccg ccggcgtatc cagcaatcag atcaacgtat cttttgaagg gcccggcagt
    24121 atccgccgca atgcggacgg tacttatacc ctgaatttag aaaactccgc cagacccggc
    24181 accaatggca cggtagtcgt cagcgccgcc ggcaattcag cccgtaaaca gttccgtata
    24241 aaagcgatcc cggatccggt tccaaggatc ggaggcatcc gcgaaaacag ggtcggcaac
    24301 ggagaattta aagcacaatt gggtatcgcc gccgtcctcg aaaactttga ttttgatgcc
    24361 aaatgcgata tcgcaggctt tacgattacc cgtgcagcca ggggtgaaga cctcatggaa
    24421 gcgcctaatc ccggttcaag atttgcaggg gaaggcatgc ggatcatcag tttggccaaa
    24481 cccggcaatt cgtatatctt ttcaaatatc cggtgcaaat gccctggcga cgaggtcaac
    24541 cggaaattat ccgacatcta tgttttaatc caataaggtc atgaaaaatt taattttagt
    24601 cctgattttt ttcgccggca gcctgggcgc agcgatggcc cagaggcccg aaaacgtgat
    24661 cacggaggcc agtgagccgg gtgacagcag catcttcatg gatgatatcg tccaccggag
    24721 gaccatattg gaccagcggg ttttgcccta tgaatccctc cgggaagccg atattccctg
    24781 ggaaaagagg ctctggcagg tcattgacgt gagagaaaaa ctgaatctac ccttcattta
    24841 tcccaaagct cccttgttcg gaatactcaa tgatgcggcg acccagggcc tggtggccgc
    24901 tttccgggat gaagatttca aacaactcat gcagcccctg gaaatcgaag gcatgatgtt
    24961 ttcgatggac accatccagg tgatcgatcc ggaaacctat gagaccaaaa cccaggtggt
    25021 aaaaaatgaa atcagcgccg caaccattaa attcttccgg atcaaggagg tctggttttt
    25081 tgaccgcgaa gcctcccgga tgaaggtcag gatcctcggc atcgcccccg tgcgcgaacg
    25141 gatcattgcc ggtaaagccg aagaacaacc gatgtactgg ctctattatc cgcagctgag
    25201 ggaaatcctg gccaggcacc gcgctttcaa cgattttaat gatgcttccc cgctcagttg
    25261 ggatgatatc tttgaattga gacggttctc cagttatgta ttcaaagaaa gcaatgccca
    25321 ggacctgagg ttaaaagact ttttcccgga ggacggcgtt gcccgcctgc gtgaaggaga
    25381 aaagatcaaa aacaaactgt ttaatttcga gcaggatttg tgggagaatt aggaggattt
    25441 attcaagaca ccggttggaa gctagaagct agaagctagt gccacgccaa ctattattgc
    25501 ctaaaaacag ctaatggttt tcggttgctg cctttttca
//
LOCUS       DAOQXT010000068        25244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2290_length_25244_cov_6.760332, whole genome shotgun sequence.
ACCESSION   DAOQXT010000068 DAOQXT010000000
VERSION     DAOQXT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 25244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25244
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2290_length_25244_cov_6.760332"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PKM27_12310"
     CDS             <1..173
                     /locus_tag="PKM27_12310"
                     /inference="COORDINATES: protein motif:HMM:NF020952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNR08094.1"
                     /translation="KLGYNRAGRIMDQLELLGVVGAGEGSKPREVLVSSEVELNRILD
                     RIRGGKERDTLF"
     gene            297..>2528
                     /locus_tag="PKM27_12315"
     CDS             297..>2528
                     /locus_tag="PKM27_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNR08095.1"
                     /translation="MIKRLLLSLFCISGTAFVLGQTGYYFPGASGFNTSIPSPEQFLG
                     YPIGSHHTRHDKVVEYIRELDRLSDRMTTRIIGQSYEQRDQIVAFITSPSNHSRIEEI
                     RQANLARANRPGGDEAPLVLTLGYNVHGNEPSSTEAALLTAWYLVASDHPETLQWLDS
                     MVILLDPVYNPDGRDRHTHWANMHKGSPPVADPNDREHNEVWPGGRTNHYWFDLNRDW
                     FLGIHPESRNRMKLFHEWMPYVMTDHHEMGSNATFYFDPGKYSSNNPIVPAYLYDVVY
                     PRYGEYFSKALNGIRSLYYTKESFDKLYPGYGSSYVNFYGGAGFLFEQASSRGHVQET
                     TTIPLEFGFTIRNQLTASLATLRASFAEKPMLLKLRRDFYAAAASQARVNPVKAYVFG
                     DAEDPSRTRAFVNLLRLHQIEVYQLDRSLQAGGKSFEKGRAYLVPVDQKNYFMVRSAF
                     EKEITYTDSLFYDASTWSLIHAFDLPFHEIRGGYATGDRIIDDLKYIPGPVEKSDYGY
                     LINQRDYQAYKAIWQLQSGNLITKVAFKPFKIQVGALSKSFGHGTLFIPVQQQKLKAD
                     EVFALMQRISSDCLIDVYPVHTGYSTEGIDLGSNFLRTLKPVKALMVIGQGVSGYEAG
                     EVWHLLDQRIGMPVTKVELNQMGRIDLNAYTTLILVHGAYAPDKPLQDRLKAWVRDGG
                     TLITFKGASEWVLRGDLTTGEKLVPADTLSPARTNYEDLQEREGAKQIGGSIYEADLD
                     LSHP"
     assembly_gap    2529..2628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2675..3913
                     /locus_tag="PKM27_12320"
     CDS             2675..3913
                     /locus_tag="PKM27_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNR08096.1"
                     /translation="MTSTIIISLCILVLIAYLFDLSSSKTKIPSVILLLVSGGLVRFL
                     TDWLGIQLPDLDVLLPVLGTVGLILIVMEGSLELQLHRSRLPMIFKSFMIAFIPLILL
                     SCLAAWGLQYLGYPHYKTNLGNVIPLFVISSAIAIPSVRHNSRPTREFVVYETSLSDI
                     LGVIFFNFIVLNEFIDQHAFFTFFMQLGLETLIAFIATLFLAFLLGKIEHHIKFVPII
                     FLIILIYAVSKELHLPALLFILIFGLFLGNLDELRNFKWIAKFHPENLDREVIRFKEI
                     TTEATFLIRSLFFLVFGYLIRPQDIINQQSAIWAACIVAAVYIVRIIILKLFRMGFDP
                     LLYIAPRGLITILLFLSIPAETRIPIINNSLVVQVMLLTVLIMMVGLMFSKEKSKEDK
                     AAREAEVRDSPEPITHSTPT"
     gene            complement(3888..4952)
                     /locus_tag="PKM27_12325"
     CDS             complement(3888..4952)
                     /locus_tag="PKM27_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HNR08097.1"
                     /translation="MNPVSGNALVFTLGLLDQNYAKTAHGLIRGSDRFTIKAVIDPVF
                     AGQEAGEIVDGRNRNIPVYATIDEALKEHRDIHYAMVGIAPVGGKLPKELLDQLKQCA
                     LKGLSIISGLHEYVSDKPDIAEAAARSGVQIIDIRKPKPKSELHFWTGRIFQVKCPIV
                     AVLGMDCAIGKRSTTRFLMEACQKEGIRAEMVFTGQTGWMQSGRYGFVLDSTYNDFVS
                     GELEHAIVCCWEEQHPDILFLEGQSALRNPSGPCGSELLISGQAKYTVLLYAPKRIYF
                     DDNPAWGKMPSLQSEIDLIRAYDSKVIGIALNTTGCTREEALRYQQSIQKEIKLPVCL
                     PVETGVGELMAAIRSVLNVL"
     gene            complement(4949..6157)
                     /locus_tag="PKM27_12330"
     CDS             complement(4949..6157)
                     /locus_tag="PKM27_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR08098.1"
                     /translation="MSLRSQFRSLIGQTSPFALELEIAGADGPWLIDPSGKKYLDMIS
                     GIGVSNLGHGNRGILDAIREQSEKYLHTMVYGEHIQSPQILLAELLVSLLPPSLNTVF
                     YLNSGAEAVEGALKLARKFTGRPGIISCKRAYHGSTYGAMSLMSDRSKTDPYRPLLPE
                     IHFMEFNRLEELEKINLQTAAVVMEVIQAEAGIRLPDPAYLEALRKRCTETGCLLIFD
                     EIQTGMGRTGSLFAFSPYGVVPDVLILGKSFGGGLPLSAFVADKKLMAVLADEPPLTH
                     MTTFGGHPLSCAAGLAALNQLVQTSLVHDSISKSDYLVKKLDQELFPALRRPPGLWLA
                     IDLADPLRVQQVIARAMNKGLLADWFLFNDESIRLAPPLTIRYEELDLAAAIINECLS
                     LEKSKSPVLS"
     gene            6263..6991
                     /locus_tag="PKM27_12335"
     CDS             6263..6991
                     /locus_tag="PKM27_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNR08099.1"
                     /translation="MRSSTALAGILLFLVACQQFVTKPLAYEIHGIDVSHFQGKVDWP
                     VVKDQGVSFAFIKATEGLEHSDTMFYTHWKALKLADIKRGAYHFFIPHLDPALQAENF
                     MINVSLQNGDLPPVLDMEVTDYPDPQDLVDRMQIWLDLVSVAYNTKPIIYTNLKAYFR
                     FVAGRFDEYPIWIARYNEKAPDLGNKKSWNFWQYANNGRIKGIKQPVDFNAFNGSHQQ
                     LESLCLKNLPSSELMELVRRNENY"
     assembly_gap    7022..7121
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7123..8034)
                     /locus_tag="PKM27_12340"
     CDS             complement(7123..8034)
                     /locus_tag="PKM27_12340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HNR08100.1"
                     /translation="MPSTKQTIICFGEILWDMLPAGKLAGGAPMNVAFHLNRLGEKVF
                     PLTALGRDELGRELMEFLKSKNIPTGLIQWNSYPTGKVLVKLNEKNEATYTIEYPSAW
                     DHIEWTESIATLFTDQSPVLVYGSLACRHEVSRNTLERLLPGAAIRICDINLRAPFYS
                     EDLIHDLLSKADWIKINHEELDLINSWNAAPEPGEQDQVQQLFAQYPRASLILVTRGG
                     DGAACYEKNSTHDHPGFKVEVQDTIGSGDSFLAMFISRKLSGFPLQVCLEDACALGAV
                     VASHPGATAIFDAESEVKKLKELRKLN"
     gene            complement(8079..9002)
                     /locus_tag="PKM27_12345"
     CDS             complement(8079..9002)
                     /locus_tag="PKM27_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR08101.1"
                     /translation="MLSLGFVTAILGDHSLEEVFDFASGNGFKCIEVMCWPASGTDTR
                     RYAGVCHIDVDRLDEGAIARIKGLQDQYQVKISGLGYYPNPLDPDPVQSAFYIDHICK
                     VIKACRALEVPVMNTFIGRDPGQSIEYNLDRFKEVWPAIVAYAESLEIRIGIENCPML
                     FTRDEWPGGKNLATTPAIWDRMFEIIPSPTLGLNYDPSHLIWQRMDEVQPVYDYKERL
                     HHIHLKDAKVYADRLNRHGIMAYPLQYHSPKLPGLGDVRWRDFFSALTSVRYRGPVCI
                     EVEDKAYEGSREDVYASIKTAKKFVSQFLPD"
     gene            complement(9024..9779)
                     /gene="deoC"
                     /locus_tag="PKM27_12350"
     CDS             complement(9024..9779)
                     /gene="deoC"
                     /locus_tag="PKM27_12350"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNR08102.1"
                     /translation="MDYTVDELAGMIDHSLLHPTLTDEEMMDGCKTAVAYRMATVCVK
                     PYFVKKAAELTAGSNTRVCSVIGFPHGNHAIKIKVAETRLAIQDGATEIDMVVHAGKV
                     LSRDWPYIRRDIAAVNKACLKGGAILKVIFENDFLPDDKLKIKLCKICNAVGVAFVKT
                     STGYGYVKSATGDYHYRGATPEDVMLMRKYCDPSIQIKAAGGIRTLDDLLLFRSLGVT
                     RIGATSSVAILEEAKRRLGLPGFMMEPQENKGY"
     gene            complement(9820..10947)
                     /locus_tag="PKM27_12355"
     CDS             complement(9820..10947)
                     /locus_tag="PKM27_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNR08103.1"
                     /translation="MRIGINARLLLRDRLEGIGWHAWEIIRRMIRNHPEHQFILFYDR
                     REGILIPEGENIETAVKFPPSRHPLLIWWWCRALGHACRHHRIEVFYSPEVLMPSGIS
                     VPCLITLHDLSPLILPHSLPLAHRWYYRYAIKKNAGEAVHLFTVSQFTRSEILRILQV
                     HPGKISVVYNAARPSFKPITDSAGLRNRFSDGHPYFIYVGALHQRKNVDQIIAAFDAF
                     KKQEGSPHKLILAGKFMGKHRRVRKAIARSGNRADLLILGYVAEPNLGPLIAGAEALI
                     NLSEYEGFGMPLVEAFQAGTPVIAADRSCYPEICGQAGLLVNPHDTADVVQAMQKAIL
                     QRRALVEAGFLQAGKYNWEESAEQVWERIRITAQNSSHLSR"
     gene            11056..11886
                     /gene="murI"
                     /locus_tag="PKM27_12360"
     CDS             11056..11886
                     /gene="murI"
                     /locus_tag="PKM27_12360"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNR08104.1"
                     /translation="MDSGPIGIFDSGYGGLTILRDLISALPGYDYLYLGDNARAPYGQ
                     RSFNTIYEYTLESVRWLFSQACPLVILACNTASAKALKNIQLFALPELAPDRKVLGVI
                     RPTTEIIGNYTASKHIGILATTGTVVSGSYLMEIKHFFPEINVFQQACPLWVPLIENN
                     EFQNPGADYFIEKYVDQLLAQSPEIDMILLACTHYPLVRDKIINFCPPGVNLMSQGPI
                     IATSLRSYLQKHPEIESRLSKGSRYEFYTTDAESDFDSHASRYFGSPVKARHTHLDHR
                     "
     gene            11952..13556
                     /gene="nadB"
                     /locus_tag="PKM27_12365"
     CDS             11952..13556
                     /gene="nadB"
                     /locus_tag="PKM27_12365"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNR08105.1"
                     /translation="MNTTDVLIIGSGVAGLSTAIQLAILRKDLSITVLTKTVQGESNT
                     RYAQGGIAAVWDETKDNFDKHIADTLDAGAGLCNQESVYNVVTEGPARVQEIIDWGTR
                     FDKTKSDLDYDLGREGGHSEHRILHYKDLSGAEIQRALIAKAASFANIVIKEHYYAIN
                     LITQHHLGYSLTRVSPNIECYGAYVLNKASKEIETWIARATILATGGTGQIYKSTTNP
                     QIATGDGIAMVYRAKGRVENMEFVQFHPTALFNPTGENPDFLVSEAVRGFGGILKTRD
                     GLEFMHKYDARKSLAPRDIVARAIDNEMKIRGDDFVYLDCRHLDRDAFIAHFPTIHEK
                     CLSIGIDPMKAMIPVVPACHYMCGGIQVDQLGRSSIKYLHACGECSSTGLHGANRLAS
                     NSLLEALVYSKRIAAYLADHLDGIGLRTQLPEWNASGTTEPKEMVLITQSWKELKEIM
                     SSYVGIVRSNVRLKRAMDRLHILYHETEDLYNTTVLSPQLCELRNLISIGYLVTRSAS
                     LRKESRGLHFTTDYPKEYGFLENTLL"
     gene            complement(13660..14016)
                     /locus_tag="PKM27_12370"
     CDS             complement(13660..14016)
                     /locus_tag="PKM27_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR08106.1"
                     /translation="MLYSLTPMLETHDLPATLRFYTEILSFNVDAYEEEWGWASLSKD
                     EITIMFSRPNPHRNLPSAILSGSLYIKTDQVDQWWEKLSLSCNVCYELETFEYGMREF
                     AIYDNNGYLLQFGQEV"
     gene            complement(14028..14387)
                     /locus_tag="PKM27_12375"
     CDS             complement(14028..14387)
                     /locus_tag="PKM27_12375"
                     /inference="COORDINATES: protein motif:HMM:NF026420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HNR08107.1"
                     /translation="MKIGYFIFGLLFVAFAAVQYNDPDPYIWGSYYLFIAILCFLEAL
                     KRYYSLLVWPAMLLTLAWAGFYLPDVRDYFQQGSPNIAGQMKAETPYIENMREFLGLM
                     ICALVLGSILYRNKKGK"
     assembly_gap    14540..14639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14640..16490)
                     /locus_tag="PKM27_12380"
     CDS             complement(<14640..16490)
                     /locus_tag="PKM27_12380"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR08108.1"
                     /translation="MKALSSAVFLALPFFIQSQILFNGYVRDAYTQTALAGARVRIED
                     LAQASRESLTDSLGRFRFEGVVAGRHQILVSLEGYQETRISDYLIGRTNIPLFIDLEP
                     RVSDLPEVTITGRSTGAFLHPLSNKLVISREQTERLSAGFFDPARLFTNQAGISPLND
                     AANHLVIRGNNPAFVKWMIHGTEIINPNHLSNAGTFSDQSSASGGGVNMISASVLNNT
                     YLNKAPSPAGLGNALAGLVDVNLRPGRTDKLTTEAQISLLGMEAGLEGPISADRGSSF
                     LFRYRYSTVGLLSELGAKFGEEDINYQDLMGHLSFPSKNSDWNIFFFTGDHQNKYAGI
                     EDSTQRTIDKESQDILYDGGQAIAGISNEWRLGAMHRWVNDLVWSQARSLREFGPAGS
                     MVLGNVNRLRQEKWSLHSRVVSAFKNNAVLTAGLQAQSNLDQAHYEDVVPRPNFDCCP
                     DPYYSIQPYAHYSVRLQNWTLGAGLHSMIMNAVSIEPRFQLQYQLSGSQSLQLNAGKH
                     SQVPSPISYYPKELRDPMVSYQAQAGYRKTNARSGWGLAFYYQYHRDIPNSGEYYSLI
                     NDYPYDNSFIAQAGSKGRIYGLETDFQAPLDKWNWTGNLSLFRSQLIAPSG"
     gene            16574..17026
                     /gene="arsN2"
                     /locus_tag="PKM27_12385"
     CDS             16574..17026
                     /gene="arsN2"
                     /locus_tag="PKM27_12385"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HNR08109.1"
                     /translation="MEVAKPPFEIFPATALDFNKIREALKQAKLVYQDLDPALRHFFV
                     ARANGQFQGCIGLEHYGHSGLLRSMWVDEGYRNQGIAGALVAKLEGYASSLGLHALYL
                     LTETAESYFGKRGFQQMDRDLTPEELKASAEFKSLCPASAKLMVKWLN"
     gene            17162..18898
                     /locus_tag="PKM27_12390"
     CDS             17162..18898
                     /locus_tag="PKM27_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNR08110.1"
                     /translation="MQSLQIKESPTEYDVVIVGSGAGGGMATYILGQAGLKICLLEAG
                     PMYDPKKDVTQFKWNWESPRRGASTKFRNFGDYDACYGGWELDGEPYTQKPGSRFDWF
                     RARMMGGRTNHWGRISLRFGPRDFKHRSLDGLGEDWPIGYEDLKPYYDRVDQMIGVFG
                     TNEGLVNDPDGYFLPPPKPRLHELYIKNGAMKAGVNVIPSRLSMLTKKINDERGVCVY
                     CGQCGRACQYYADFSASSCLVIPALKNKNVELVCDAMAREVTTNAEGLATGVSYVDRN
                     TLQEYHIKAKAVILAASACESARLLLNSKSSAHPNGLGNSSNIVGKYLHDSTGKDMGG
                     VLPRLMDHERYNEDGVGGAHIYSPWWLDNKKLDFPRGYHIEYWGGMGMPGYGFMGGIE
                     NLNGKFPVKGKTKEAGGYGASLKEDYRAFYGAMVGMSGRGEAIAREDNYCEIDPNVVD
                     KYGIPVLRFHYKWSDYEIKQAKHMQDTFQEILTQMGATITFGVDSKENNWGLAAPGRI
                     IHEVGTVRMHDDPKKGPLNKNCQAHDCKNLFVADAGPFVSQADKNPTWTIMALSMRTS
                     EYILDQMKKREI"
     gene            19091..>19757
                     /locus_tag="PKM27_12395"
     CDS             19091..>19757
                     /locus_tag="PKM27_12395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HNR08111.1"
                     /translation="MKRRESLKTIALGTVTAAALLKSCKAEEPKPGDTSASPAAPGKT
                     PPEGPARRPEEIAHYKEVISKTFFTTDEMASIAILADIIIPKDEVSGSATEAGVPDFI
                     EFIVKDQPHYQVPMRGGLRWMDLQCLQRFQKPFKDCSAAQQLEVVDLVAYPKKARPEH
                     SQGVSFFNTMRNLTATGFYTSAMGVKDIGFKGNTPNQWAGVPEDVLKAHGLAYTEKEL
                     KECI"
     assembly_gap    19758..19857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19858..20503
                     /locus_tag="PKM27_12400"
     CDS             <19858..20503
                     /locus_tag="PKM27_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR08112.1"
                     /translation="LFNTHYHGDHSGGNENFANMGATIIAHQNVRARLSADQRIINFS
                     QPQKAAPEAAWPVITFGDELNVFLNGEEVMIFHVEHAHTDGDALYYLPRSNVLHTGDC
                     FMKDRFPFIDRNSGGSVDGWLAAASRTLTLIDEETIIIPGHGDLANKKDYRDARDAVM
                     TLRDAVKGKIREGLDLDQIKALNLGKPFDTTFGSGFIKSDALVTTIYKDEMGEK"
     gene            complement(20591..21133)
                     /locus_tag="PKM27_12405"
     CDS             complement(20591..21133)
                     /locus_tag="PKM27_12405"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNR08113.1"
                     /translation="MSDPANPWTTLTEKIMYENPWIRVEEHEVINPSGNPGIYGKIHF
                     KNIAVAVLPLDDQGYTWLVGQYRYPLDSYEWEIPEGGCKEGESTLAAAQRELAEETGL
                     RATRYENILEMQLSNSVSDEVSVSYLATGLTEGTAYPEEDEKIQIRKVHFHEAVEMVK
                     KGEIRDALSVATILKAALWM"
     gene            21181..21717
                     /locus_tag="PKM27_12410"
     CDS             21181..21717
                     /locus_tag="PKM27_12410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNR08114.1"
                     /translation="MDLKLLAHLPEFHVHPESKLIVRFQDCDPFRHLNQSRYLDYFVN
                     AREDQLREHYGLDIYRFAMENGLAWMVVRNQIAYLRQVMVNEIVTIQTHVIEFDRQNL
                     TVEMIMWDENKIKPKALLWGTFAFVNLLEGRRAEHPDEMMEFYTKVHWNGAHGQSFDQ
                     RIQALYAIGKEKKPVQPG"
     gene            complement(21705..22838)
                     /locus_tag="PKM27_12415"
     CDS             complement(21705..22838)
                     /locus_tag="PKM27_12415"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR08115.1"
                     /translation="MDSPCLHIVSLDIPYPPLRGNVSGIYHRIRSLKEAGVEVILHAF
                     YKKFSLPDELTKYCRTIYLYPRKAMWKITGLRQPLFVQSRRSAQLLDMLCQDKHPILF
                     EGLHCLYHFEDPRLADRKKFIRMHNIESLYYGHLGRTETHLLRKGYFRFESSRSKSLE
                     EKGLPKATGVFAISQAEHHYLSIRNIPSVWIPPFHPHREAGGKAGFGEYALYHGDLSI
                     RENEEAAVFLIRQVFSKLDYPLVIAGFRPGIKLKREMEGRQQITLIDSPSTVQMREIL
                     EKAHIHLLPFSQNNGFKMKILDSLAFGRHILSNTLIGEANPELAGCLHLAGPSAPEWI
                     EKIKTLSQQAYLPEESDKRQNLFDSIYNNAISVKKILNAIYPG"
     gene            23101..>25244
                     /locus_tag="PKM27_12420"
     CDS             23101..>25244
                     /locus_tag="PKM27_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNR08116.1"
                     /translation="MVLILVSAAVYSAIAQARFASIQEALFSAGKMNGQGGPPNVNWI
                     RDGNQFSYIKGAGKINTYNPSTDTDEEVFNAGSLKFPGTDKEFAYTGFEWTADFNYLL
                     FQTNFQPVWRNSGNADYYLFSFKDKSLRLVAQGAYTAQVSPDGRSVAYERNGNLFVLD
                     LASKKEKQLTRDGAASVYNGRFGWVYEEEFGLVRAWEWNPDSKSIAYWRIDERAIPMY
                     QYSDLSGMHPEYKKIPYPRVGDPVPLARIGIVDIKKGKTTWANIPLQGGYFPRIYWTS
                     LPNTLAVARLDRKQKRLDLFMVDSRNGTARNILTEDSKGWMDVYNFFAGILHHFFFPP
                     RVEEFYWISDRSGYQHLYRYDYSGKLISQVTQGNWDVMTVESLDPATGLLYYTSTEAS
                     GLERHLYSIGLDGSSKRKLTLQPGRHQIQFAPGGQYYIDTWSNTTTPRQVEIRKVNGQ
                     LIKIWQDNAAVKTYVSSIAYAPRELETVVTSDGRTLDIYLIKPVGLDVSKKYPLVLSI
                     YGGPGAQSVYNSWAANPYEQYLAQQGYAIASVNNRGSTGYGRDFMESVYEQIGKLETE
                     DFVATADALARKYSWLDRQRMAIQGHSYGGFTTCYTLTKHPGAFRVGIAGAPVVNQKL
                     YDNIYSERYMGILPENDQKYEDHAPLNFVKNLSDPLLIAHALEDDNVHVINTFQFVKA
                     ATDAGKDVDLRIYPPGHHGVAYSAVSQIRLWQT"
BASE COUNT         5990 a         6413 c         6408 g         6033 t
ORIGIN      
        1 tcaaactcgg ttataaccgg gccggccgca tcatggatca gcttgaactg cttggtgtgg
       61 tcggcgcggg agaaggttca aaaccgagag aagtcctggt cagcagcgag gtagaactca
      121 acaggatcct ggaccggatc cggggtggta aggaaaggga tactttgttt tagttcgtga
      181 gttcgtaagt tcgtaagttg ggggtgtgca cctttcctca ggataaagtg ctgattttat
      241 ttcgatttgg catttccttc catccctctt atcttgagaa tcttatattg ttaccgatga
      301 taaaaagact ccttttatcc ctgttttgca tttccgggac agcctttgtt ttaggtcaaa
      361 ccggttatta tttccccggg gcatccggat tcaatacttc catccccagc ccggagcaat
      421 tcctgggtta tcccattggc agccatcata cccggcacga taaagtggtt gaatacatca
      481 gggaactgga ccgtctcagt gaccggatga ctaccaggat catcggacag tcttatgagc
      541 aaagggacca gatcgtggct tttatcacct cgccctccaa ccactcccgg atcgaggaga
      601 tccggcaggc caatcttgcc cgggccaacc ggcccggagg cgatgaggct cccctggtcc
      661 tcaccctggg atacaacgtc catggaaacg aaccctcgag cacggaagca gccctgctca
      721 cggcctggta cctggtagcc agcgatcatc ccgaaaccct gcaatggctg gacagcatgg
      781 tcatcctgct ggatcccgtt tataatcccg atggccgcga caggcatacg cattgggcca
      841 atatgcacaa aggcagcccg ccggtagccg atcccaacga ccgggaacac aatgaggtct
      901 ggcccggggg ccggaccaac cattattggt ttgacctcaa ccgggactgg tttttaggca
      961 tccatccgga aagccgcaac cggatgaaac tctttcacga atggatgccc tatgtgatga
     1021 cggatcacca cgagatgggc tccaacgcca ccttttattt tgatccgggc aaatacagca
     1081 gcaacaatcc catcgtgccg gcttacctct acgatgtggt ctatcccaga tacggcgaat
     1141 atttttccaa ggccttgaat ggtatccggt cgctctatta taccaaggag tcctttgaca
     1201 agttgtatcc gggttatggg tccagttacg tcaattttta cggaggagct ggatttttgt
     1261 ttgagcaggc cagttccaga gggcatgtgc aggagaccac taccattcca cttgaatttg
     1321 ggtttaccat ccgcaaccag cttaccgcct ccctggctac cctgcgggcc tcatttgccg
     1381 aaaagcccat gctgctcaaa ttacgccgtg atttttatgc cgcagccgct tcccaggcca
     1441 gggttaatcc ggtaaaagca tatgtgttcg gggatgcgga agacccttcc aggacgaggg
     1501 cctttgtcaa cctcctgcgc ctgcaccaga tcgaggtgta tcaactggac cggagcctgc
     1561 aggccggcgg caaaagtttt gaaaaaggca gagcctacct ggtgccggtg gatcagaaaa
     1621 actattttat ggtccgttcc gctttcgaaa aggagattac ctataccgat agcttgtttt
     1681 acgatgcttc cacctggtcc ctgatccatg cctttgatct tccttttcac gaaatccggg
     1741 gaggctatgc cactggagac aggataatag atgatctgaa atacattcct ggtccggtcg
     1801 aaaaaagcga ttacggatac cttatcaacc aacgcgatta ccaggcctat aaagcgatct
     1861 ggcagctaca atccggaaac cttatcacca aagtcgcttt caaacccttt aaaatacagg
     1921 ttggcgcttt atccaaatcc ttcggacacg ggaccttgtt tatacccgtc cagcaacaga
     1981 aactgaaagc cgatgaagtc tttgccctga tgcaacgcat ttccagcgac tgcctgatcg
     2041 atgtttatcc cgtgcatacc gggtattcca cggaagggat agacctggga tcgaattttt
     2101 taaggacttt gaaaccggtc aaagccctta tggtcattgg ccagggagtc agcggatacg
     2161 aagccgggga ggtctggcat ctgctggacc agcgcatcgg catgccggtg accaaggtgg
     2221 aattgaacca gatgggcagg atcgacctca atgcctatac cacccttatc cttgttcatg
     2281 gcgcttatgc accggacaag cccttgcagg accggttgaa ggcctgggtc cgcgacggag
     2341 gcaccctcat caccttcaag ggagcctctg aatgggttct ccggggggat ttgactacgg
     2401 gcgaaaaact tgtcccggcg gatacccttt ccccggcaag aacaaattat gaagacctgc
     2461 aggaacggga aggtgcaaaa caaataggcg gctcaatcta tgaagcggac ctggacctca
     2521 gtcatcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aggttgaatt
     2641 ttcgagtatc ttgaagattt attaaaaaga ataaatgact tcaaccatca tcatttccct
     2701 gtgcattctg gtcctcattg cctatttatt tgatttaagt tcttccaaga ccaagatacc
     2761 ttcggtgatt ctgttgttgg tatccggagg cctcgtccgt tttctgacgg attggttggg
     2821 gatccaattg ccggatctgg acgttttgct cccggttttg ggtacggtcg gacttattct
     2881 catcgtgatg gaaggatcgc tggaactgca actgcacaga tccaggcttc cgatgatatt
     2941 caaatccttt atgatcgctt ttattccttt gattcttttg tcttgcctgg ctgcctgggg
     3001 tctgcaatac ctgggatatc cccattacaa gaccaatctt gggaatgtga tcccgctgtt
     3061 tgtaatcagc agcgctattg ctattcccag cgtccgacac aacagccggc ctacccggga
     3121 atttgtagtt tacgaaacca gtctgtctga cattctggga gtcatatttt ttaattttat
     3181 cgtattgaat gagttcattg accagcacgc tttcttcact tttttcatgc agttgggcct
     3241 cgagaccctg attgcattta tcgccacctt attccttgcc ttcctgctgg gaaaaattga
     3301 acaccacatt aaatttgttc caatcatatt cctcatcatc ctcatttatg ccgtttcgaa
     3361 ggaattacat ttgccagccc tgttattcat cctgattttt ggtttgtttc tcggcaacct
     3421 ggatgaactt agaaatttca aatggatagc aaaattccat ccggaaaacc tggatcggga
     3481 agtcatccgc tttaaggaaa ttacgaccga agccaccttt ctgatccgtt ccctgttttt
     3541 cctggtgttt ggctacctga tccggccaca ggacatcatc aatcaacaaa gtgccatttg
     3601 ggcggcctgt attgttgcgg ccgtgtacat cgtccggatc attatcctta aactgttcag
     3661 aatgggtttt gaccctttgt tgtacatcgc cccccgcggt ttgatcacca tcctcctgtt
     3721 tttatccata cctgctgaaa cccgaatccc gattataaac aattccctcg tggttcaggt
     3781 gatgctgctg accgtattga ttatgatggt tggtctgatg tttagtaagg agaagagtaa
     3841 ggaggacaaa gcggcccggg aggcggaggt acgggattcc cctgaaccta taacacattc
     3901 aacaccgacc tgatcgccgc catcagttct ccgacacccg tttccaccgg caggcaaaca
     3961 ggcagtttga tttctttttg gatgctttgc tggtaccgga gggcttcctc tcgggtacat
     4021 ccggttgtat ttaaggcgat gccgatcacc ttgctgtcgt aggcccggat aaggtctatt
     4081 tcagactgca gggagggcat tttaccccag gccggattgt cgtcaaaata aatgcgtttg
     4141 ggagcatata ggagtacggt gtactttgcc tggccggaga tcagcaattc cgatccgcag
     4201 gggccgcttg gattccggag ggcggattgt ccttccagga agaggatgtc cggatgctgc
     4261 tcttcccagc aacagacgat ggcgtgttcc agttccccgg aaacaaaatc attgtaggtg
     4321 gagtccagga caaagccgta ccggccgctt tgcatccatc ccgtttgccc ggtaaaaacc
     4381 atttcggccc tgatcccttc cttttgacag gcctccatca gaaaacgggt ggtgcttctt
     4441 ttccctatgg cgcagtccat gccgaggaca gccacgatgg ggcatttgac ctggaagatg
     4501 cgtcccgtcc agaaatgcag ttcggatttg ggtttgggtt tccggatgtc gatgatttgt
     4561 acaccggacc gggcggcggc ttccgcgata tccggtttgt cgctgacgta ttcatgcagg
     4621 ccgctgatga tggacaaacc tttaagagcg cattgtttca gttggtccag taattcttta
     4681 ggcagtttac cacctaccgg agcaattccg accatggcat agtggatgtc ccggtgttcc
     4741 ttaagtgctt catcgatcgt cgcatatacg gggatgttcc ggttccttcc gtccacgatt
     4801 tcccccgcct cctggcctgc aaaaaccgga tcgatgacgg ctttgattgt aaaccggtcg
     4861 gaaccccgga tgagtccatg ggccgtcttg gcgtaattct ggtccaataa tccgagggtg
     4921 aatacaagcg cgtttccgga aacaggattc atgataggac gggtgatttt gatttttcaa
     4981 gggaaaggca ttcgttgatt atggccgcgg ccaggtccag ttcttcgtaa cggatggtca
     5041 ggggcggagc caggcggatg gattcatcat tgaacaaaaa ccaatcggcc agcaaacctt
     5101 tattcatggc ccgggcaata acctgttgta cccgcagcgg gtcggcaagg tcaatcgcca
     5161 gccaaagacc gggtgggcgg cgtaaggcgg ggaacagctc ctgatccagc tttttgacca
     5221 ggtaatccga tttggaaatg ctgtcgtgca ccaggctggt ttggactaac tgattaagcg
     5281 cggccagccc ggcggcgcag gacaagggat gcccgccaaa ggtggtcata tgcgtgagcg
     5341 gcggttcatc cgcaaggacg gccatcaatt ttttgtccgc cacaaaagcg gacaaaggca
     5401 agcctccccc aaaactttta cccaggatca gaacatccgg caccactcca taagggctga
     5461 aggcaaacaa ggatccggtc ctccccatcc cggtctggat ttcatcgaag atcaggaggc
     5521 aaccggtttc cgtgcatctt tttctaagcg cttccaggta ggcgggatca ggaaggcgga
     5581 ttcccgcttc cgcctggatc acctccatca ctacggcggc cgtttgcagg tttatttttt
     5641 cgagttcttc caggcgattg aattccataa aatggatttc cggcagcaaa ggccggtacg
     5701 gatcggtttt actgcggtca ctcatcaggc tcatggcgcc ataagtactg ccgtggtaag
     5761 cgcgtttgca gcttatgatc cccggtctgc cggtaaattt gcgggccagt ttcagggccc
     5821 cttccacggc ttctgccccg ctgttgaggt aaaagacggt gttcagggaa ggaggaagca
     5881 ggctgaccag gagttcggcc aggaggattt gcggcgactg gatgtgctcc ccatagacca
     5941 tggtatgcag atatttttcc gattgttccc ggatggcatc caggatgccc cggtttccat
     6001 ggcccaggtt gctcaccccg attccggaaa tcatatccag gtattttttc ccgcttggat
     6061 cgataagcca gggtccatcg gcccccgcga tctccaattc cagggcaaaa gggctggtct
     6121 ggccgatcag ggaacggaat tggctgcgaa ggctcattcg gacaatgaaa tttatcaggc
     6181 ctaaatatgc gttaacttac aaaataaatg agcgcccctc ccaatttttc attaagattg
     6241 gggtgacaat tataagttct ccatgcgatc aagcactgca cttgcgggaa tcctgctttt
     6301 tctggtagcc tgtcagcagt ttgtcaccaa acccctggct tatgagatcc atggtattga
     6361 tgtttcccat tttcagggca aggtagactg gccggtggtg aaggaccagg gggtttcctt
     6421 tgcctttata aaagcgacgg agggacttga acacagcgat actatgttct atacccattg
     6481 gaaggccctg aaactggccg atatcaaacg cggggcctat cattttttta ttccccatct
     6541 cgatcccgcc ctccaggcgg aaaattttat gatcaatgtg agcctccaga acggggacct
     6601 gcccccggta ttggatatgg aggttacaga ttaccccgat ccgcaggacc tggtggaccg
     6661 aatgcagatc tggctggacc tggtatctgt cgcttacaat accaagccga tcatctatac
     6721 caatctcaaa gcttatttcc gctttgtcgc cgggcgtttt gacgaatatc ccatctggat
     6781 tgcccgctac aacgaaaagg ccccggacct tggaaacaaa aaatcctgga atttctggca
     6841 atatgccaac aacgggcgga tcaaagggat caagcagccg gttgatttta acgctttcaa
     6901 tggaagccac caacaattgg agtccctttg tttaaaaaac cttccttcct ctgaattgat
     6961 ggaactggtt agaagaaatg aaaactatta gcaagttagc aagttagcaa gttagcaagt
     7021 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattaattta attttcttaa
     7141 ttcttttaat ttctttactt cgctttctgc atcaaaaatg gctgtggccc ctggatggct
     7201 tgccaccacc gctccaagag cacaggcatc ttccaggcag acctgcaggg gaaaacccga
     7261 aagttttctg gagataaaca tggccaaaaa cgaatcgccg cttccgatgg tgtcctgcac
     7321 ttcaacctta aagcccggat ggtcgtgggt actgtttttt tcatagcagg cggccccatc
     7381 cccgccgcgg gtgaccagga tcaggcttgc ccggggatat tgggcaaaca attgctgaac
     7441 ctggtcttgt tcgccaggct ccggagcggc gttccacgaa ttgatcaggt ccagctcctc
     7501 atgattgatc tttatccagt cggctttgga taaaagatca tgaatcaggt cctctgaata
     7561 aaaaggggcg cggaggttga tatcgcaaat cctgatcgcg gctccgggaa gcaatcgttc
     7621 cagggtattt ctgctgactt catgtcgaca ggccagagaa ccatagacca ggaccgggga
     7681 ctgatcggta aataaagttg cgatggattc ggtccattcg atgtgatccc aggcggaagg
     7741 atattcaatg gtgtacgtcg cttcgttttt ttcattgagt ttcaccagga ccttgcccgt
     7801 ggggtaggaa ttccattgaa ttaatccggt tggaatattt ttactcttca gaaattccat
     7861 caattcgcga cccagttcat cccggcccag ggccgtcagg gggaaaacct tttcacccag
     7921 gcggtttaaa tgaaaagcca cattcatggg ggcgccccct gccagtttgc ccgccggcaa
     7981 catatcccag agtatttccc cgaagcaaat gatggtttgt ttggttgaag gcatggctga
     8041 tgggtaggcc ttatttcagt tattgacaat ccgggatatt aatccggcag aaactgggaa
     8101 acaaactttt ttgccgtttt gatggaagca tagacgtctt ccctgcttcc ttcgtaggct
     8161 ttgtcttcca cttcaataca gaccgggccg cggtaacgga cgctggtcag cgcggaaaaa
     8221 aagtcccgcc atcgcacatc acccagcccg ggcagtttcg gactgtggta ctggagggga
     8281 taggccatga ttccatgccg gttcaagcgg tcggcataga ccttggcgtc cttgaggtgg
     8341 atgtggtgca gcctttcttt ataatcataa acaggctgaa cttcatccat tctttgccag
     8401 atcaggtggg aaggatcata attgagcccc agggtcgggg aagggatgat ttcgaacatc
     8461 cggtcccaga tggccggagt ggtggccagg tttttgcctc cgggccattc atcccgggta
     8521 aacagcatcg gacagttttc aatcccgatc ctgatctcca ggctttcggc atacgctacg
     8581 atcgcaggcc agacttcttt aaaacggtcc aggttgtatt caatcgactg gccggggtcc
     8641 ctgccgataa aggtgttcat gaccgggacc tccaaggccc ggcaggcttt gatcacttta
     8701 catatgtgat caatgtaaaa agcggattgc acgggatcgg gatccagggg attggggtaa
     8761 taacccaggc cggatatttt tacctgatat tggtcctgca ggcctttgat ccgcgcgatg
     8821 gccccttcat ccagacggtc cacatcaata tgacagactc cggcataacg gcgggtatct
     8881 gtacccgagg ccggccagca catcacctca atgcatttga atccattccc ggaagcaaaa
     8941 tcaaaaactt cttccaggga atgatccccg agaatagcgg ttacaaaacc cagtgagagc
     9001 atgaacgata gacgattggt tgattaatac cctttatttt cctgcggttc catcataaaa
     9061 cccgggaggc ccagtcttct tttggcttcc tccaatatgg ccacactgga ggtcgcacca
     9121 atgcgggtga cgcccaggga ccggaagagc aagaggtcgt ccagtgtgcg gattccccca
     9181 gctgctttga tctgtatgga aggatcacaa tatttcctca tgagcatcac atcctccggg
     9241 gtggcccccc ggtaatgata atccccagtg gccgatttga cataaccgta tcccgtactg
     9301 gtttttacaa aggctacccc gaccgcattg caaatcttgc acagcttgat cttgagtttg
     9361 tcatccggta aaaagtcatt ttcaaagatc actttaagga ttgcgccgcc ttttaaacag
     9421 gccttgttca ccgcagcgat atcccgccgg atatagggcc agtccctgct caaaactttg
     9481 ccggcgtgca cgaccatgtc tatttcggtc gccccgtcct ggatagccag gcgggtttcg
     9541 gcgaccttga ttttgatggc atggttgcca tgagggaagc cgatcaccga acaaaccctg
     9601 gtattgctgc ccgctgtaag ctccgcagct tttttaacaa aataaggttt gacacagact
     9661 gtagccatcc ggtaggcaac agcggtcttg cagccgtcca tcatctcctc atcggtaagg
     9721 gtgggatgga ggagggaatg atcgatcatg cctgccaatt catctacggt atagtccata
     9781 tccttgtttg gtgtgaagtt aaagagaaaa tggatgcgat caacgggaga ggtgagatga
     9841 gttttgagct gtgatcctta tcctctccca tacttgttcc gcggactcct cccagttata
     9901 tttcccggcc tggagaaaac cggcttccac caacgcccgt ctttgaagga tcgctttttg
     9961 catggcttgg actacgtcgg cagtatcgtg cggattgacc agaaggcctg cctggccaca
    10021 gatctccgga taacaggacc ggtcggccgc gatcacgggc gtaccggcct ggaaggcctc
    10081 gaccaggggc atgccaaagc cctcgtattc cgaaaggttg atcagggcct cggcccctgc
    10141 gatcaaaggg ccaaggttgg gttccgcgac gtagccaagt atgaggaggt ccgccctgtt
    10201 tccggatctt gcaatggcct tacgaacgcg ccggtgtttg cccataaatt ttcctgccaa
    10261 gatcagtttg tgaggactgc cctcctgttt tttgaaggca tcaaaggccg cgatgatttg
    10321 atctacattt ttacgttgat gcaaggcgcc cacataaatg aaatagggat gtccatccga
    10381 aaatcgattg cggaggcctg cggaatccgt tatgggttta aaggaaggcc gggccgcatt
    10441 atacaccaca ctgatctttc ccgggtggac ctgcaaaatc ctgaggattt cagacctggt
    10501 gaattgactc acggtgaaca ggtggacggc ttcccccgca ttctttttta tagcgtatcg
    10561 gtaataccac cggtgggcaa gggggaggct gtgcggcagg atcaggggcg ataggtcatg
    10621 gagggtgatc aggcagggca cagagatccc ggatggcatc aacacttccg gggaataaaa
    10681 gacctcgatg cggtgatgcc tgcaagcatg gccaagggcc ctgcaccacc accagatcaa
    10741 caggggatgg cgactcggcg gaaacttcac agcggtttca atgttttccc cttcagggat
    10801 caggatgccc tccctgcggt cataaaaaag gataaattgg tgttccggat ggttccggat
    10861 catgcgccgg atgatttccc aggcatgcca tcctataccc tcgagccggt cgcggaggag
    10921 gaggcgggca ttgattccga tgcgcatcgg gcaaaaataa ccattgcctt tgatgattga
    10981 tttggccgag ggggcccgga tgaccaaaga cctggtcctt tccgactctg caaaaatctt
    11041 atttttgcag agtccatgga cagcgggccg attggtattt ttgattcggg ttatggtggg
    11101 ctcaccattc tcagggacct gatatccgct ctgcccggtt acgactatct ctacctggga
    11161 gacaatgcca gggctcccta tggccagcgc tccttcaaca ccatttacga atacaccctt
    11221 gaaagcgtcc gctggttgtt cagccaggca tgccccctgg tgatcctggc ctgcaacacg
    11281 gcctcggcca aagcgcttaa aaacatccag ctttttgctt tgccggaatt ggccccggac
    11341 cggaaagtgt tgggggtgat caggcctaca acggaaatca tcggcaacta tacggccagc
    11401 aaacacattg gcatcctggc caccacgggc actgtggttt cagggtctta ccttatggag
    11461 atcaaacatt ttttcccgga gatcaatgtt tttcaacagg cctgtccgct ttgggtgcct
    11521 ttgatcgaaa acaatgaatt ccagaatccg ggcgcggatt attttatcga aaaatatgtc
    11581 gaccaattgc tggcgcagtc cccggagatc gatatgatcc tgctggcctg tacccattat
    11641 cccctggtgc gtgataaaat cataaacttt tgccctcccg gggtcaatct catgagccag
    11701 ggacccatca ttgccaccag cctgaggtca tatcttcaaa aacatccgga aatcgaatcc
    11761 aggctgagta aaggaagcag gtatgaattc tataccaccg atgcggaatc cgattttgat
    11821 tcccatgcga gccggtattt tggttctccg gtaaaagccc ggcataccca cctggatcat
    11881 cgctaagtca gatataccaa atcatcaaga tatggcatat ccgaagtact tttaaggctt
    11941 aaatcctcat tttgaataca accgatgtct tgatcattgg atccggtgtg gcgggattgt
    12001 caacagccat ccagctggct attctccgta aggacctcag cattacggtc ctgaccaaga
    12061 ccgtccaggg ggaaagcaat acccggtatg cccagggggg aatcgctgcc gtctgggacg
    12121 agaccaagga taattttgac aaacacatcg cggataccct ggatgccggg gccggactct
    12181 gcaatcagga atcggtctac aacgtcgtca ccgaaggccc cgcgcgggtg caggaaatca
    12241 ttgactgggg cacccgtttc gataaaacca aatcggacct cgattatgac ctgggccggg
    12301 aaggcggcca cagcgaacac cggatccttc attataaaga cctcagcggg gcggagatcc
    12361 agcgcgccct gatcgccaaa gcggcttcct ttgcaaacat cgttataaaa gaacattatt
    12421 acgccatcaa cctgatcacc caacatcacc tgggttattc actcacccgt gtatccccca
    12481 atatcgaatg ttatggggct tatgttttga ataaggcttc caaggaaatt gaaacctgga
    12541 ttgcgcgggc gaccatcttg gccaccgggg gtaccgggca gatttacaaa tccactacca
    12601 atccccagat tgccaccggc gacgggatcg ccatggtgta cagggccaag ggcagggtgg
    12661 agaacatgga gtttgttcag tttcatccga ccgccctgtt caacccgacc ggtgaaaatc
    12721 cggatttcct cgtttctgaa gcggtgcgcg gcttcggggg catcctgaaa acccgggatg
    12781 ggcttgaatt catgcacaaa tacgatgcgc gcaaatctct cgctccccgt gatatcgtcg
    12841 cccgggcgat agacaatgaa atgaagatcc gtggggacga ttttgtatac ctggattgcc
    12901 ggcatttgga ccgggacgcc tttatagccc atttcccgac cattcatgaa aaatgcctga
    12961 gtataggcat cgatcccatg aaggccatga tcccggtcgt gcctgcctgc cattatatgt
    13021 gcggaggcat tcaggtcgac cagttggggc gttcttccat aaaatacctc catgcttgcg
    13081 gggagtgcag ctctaccggc ctgcatggag ccaatcgcct cgcctccaac tccctgctgg
    13141 aagccctggt ttacagcaaa cgcatcgcgg cttatctggc ggaccatttg gatgggatcg
    13201 gcctgcgaac ccagctcccg gaatggaacg ccagcggtac cacagaacct aaggagatgg
    13261 tgctcatcac ccaaagctgg aaggaactca aggaaattat gagttcttac gtcggtatcg
    13321 tgcgctccaa tgtgcgactc aagcgcgcca tggacaggct ccatatcctt tatcatgaaa
    13381 ccgaagattt gtacaatacc accgtgttgt ccccccaatt gtgcgagctc cgcaacctga
    13441 tttccattgg ttatttggtc acgcgctcgg cctccctgcg aaaagagagc cgcggcttgc
    13501 actttacgac agactacccg aaggaatatg gatttttgga aaataccttg ctttagaagt
    13561 caatagtcaa tagtcaatag tcaatagtca atagtcaata gtcggtggtc aagagtcaag
    13621 agtgaatagt cgaaagtcaa tggagccttg gaggaggggt cagacctctt gcccaaactg
    13681 caggagatat ccattattat catagatcgc aaactccctc attccatatt caaaagtttc
    13741 cagttcatag cagacattgc aggacaagct taatttttcc caccattggt ccacctgatc
    13801 ggttttgata tacaatgaac cgcttaggat ggcggaaggc agattgcggt gcggattggg
    13861 ccggctgaac atgatggtaa tttcatcctt ggacagggag gcccagcccc attcttcttc
    13921 ataggcgtcc acattaaaag acaggatttc agtataaaac cgaagtgtgg ccggaagatc
    13981 atgggtttcc aacatcggag tcagggaata aagcatgggc aagctcttta tttgcctttc
    14041 ttattccggt acagaatgga gcccaggacc agggcgcaaa tcatcaggcc gaggaattcg
    14101 cgcatatttt cgatataggg cgtttcggcc ttcatctggc ctgcgatatt gggggaccct
    14161 tgctgaaaat aatcccgaac atcaggcagg taaaatcctg cccaggccag ggtaaggagc
    14221 atcgcaggcc ataccaggag ggaataatat cgcttcagcg cctccaggaa acagagtatg
    14281 gctatgaaga gataataact tccccagata taggggtccg gatcgttgta ctgtacggcg
    14341 gcgaaggcta caaataacaa tccgaagata aaatacccga ttttcatggc ggtcagtatt
    14401 caatggtaaa agaaatattg ggcaggatgc cgagttgata attgaatgcg gtcttgccct
    14461 gcacataatc aaaataggag aaggaaaaat tctgcttatt cgtcaggttc tgaatatcca
    14521 gggccaggag ggtgttgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    14641 cccgaagggg cgatcagctg cgaccggaag agggaaaggt ttccggtcca gttccattta
    14701 tccagcgggg cctggaagtc tgtttccagc ccgtagatcc ggcctttgga tccggcctgg
    14761 gcgataaagg aattgtcata aggatagtca ttgatcaggg aataatattc cccgctgttg
    14821 ggaatgtccc ggtgatactg ataatagaag gcaaggcccc atccggaacg agcattggtc
    14881 ttgcggtatc cggcctgggc ctgataggag accatgggat ccctgagttc tttgggataa
    14941 taggagatgg gggaagggac ctggctgtgt ttgcctgcgt tgagttgaag ggattgactc
    15001 ccgctcagct ggtattgcaa ttgaaacctg ggttcaatgg atacggcatt catgatcatg
    15061 gaatgcaggc ctgcccccag ggtccagttt tgtaatcgga cagaataatg agcatagggc
    15121 tgaatggaat aataagggtc cggacagcaa tcgaaattgg gcctggggac cacatcctca
    15181 taatgggcct ggtcaagatt ggactgcgcc tggagtcccg ccgtcaatac cgcgttattt
    15241 ttaaaggcgg ataccactct ggaatgcagg ctccattttt cctgccggag ccggttcaca
    15301 tttccgagga ccatgctgcc tgcgggacca aattccctga gactccgtgc ctgcgaccag
    15361 accaggtcat taacccatcg gtgcatcgca cccagcctcc attcattgct gatcccggct
    15421 atagcctgcc cgccgtcata aagaatgtcc tggctttctt tatcgatggt acgttgggtg
    15481 ctgtcctcga ttccggcata tttattctga tgatctccgg taaagaaaaa aatattccaa
    15541 tctgaatttt ttgaaggaaa actgaggtgg cccataaggt cctggtagtt gatgtcctct
    15601 tccccgaatt tggcgcccag ttcagaaagc aaacctaccg tggagtaccg gtagcggaat
    15661 aaaaacgagc ttccccgatc cgcagaaatc ggtccttcca gaccggcttc catgcccaat
    15721 aaactgatct gggcctcagt ggtgagctta tcggtccggc cgggccggag gttgacatcc
    15781 acgaggcccg caagggcatt gcccaggccg gccgggctcg gtgctttatt gagataggta
    15841 ttgttcaata cggatgcact gatcatgttt acacctcctc cgctggcgct ggactggtcc
    15901 gaaaaggtac ccgcattgct caggtgattg ggattgatga tttccgtgcc gtggatcatc
    15961 catttcacaa aagcgggatt attcccccga atcaccaggt ggtttgctgc atcgttgaga
    16021 ggacttatcc cggcctggtt ggtaaataaa cgggcaggat caaaaaagcc ggcggacaag
    16081 cgctccgttt gttccctgga gatgaccagt ttattggaca agggatgcaa aaaggccccg
    16141 gtgcttctgc ccgtgatggt tacttccggg aggtccgaaa ccctcggttc gagatcaatg
    16201 aacaggggaa tattggtgcg cccgataagg taatcggata tcctggtttc ttgataacct
    16261 tcaaggctga ccaggatttg gtgtctgccg gccactaccc cttcaaaccg gaaccgtccc
    16321 agtgaatcgg tcagggattc ccggctggct tgggcaaggt cttcaattcg gaccctggcc
    16381 ccggccaaag cggtctgggt ataggcgtcc cgcacataac cattaaacag gatttgggac
    16441 tggataaaaa aaggaagcgc cagaaaaacg gcggacgaga gggccttcaa gcatggtaca
    16501 aatttgatcg tcatatcggc ccgtaaagtt aatttcttat cctgccgatt tcagttattt
    16561 ttatttttag aatatggaag ttgcaaaacc acccttcgaa atcttccctg ccacagccct
    16621 tgattttaat aagatccgtg aggccttaaa acaggcaaaa ctggtctacc aggatctgga
    16681 ccccgcgctc cggcattttt ttgtcgcccg agcaaatggg caattccagg gttgcatcgg
    16741 cctggagcac tatggccatt ccggtttatt gagaagcatg tgggtggacg aagggtaccg
    16801 gaaccagggc attgccggcg ccctggtcgc taagttggaa ggctatgcct cctccctggg
    16861 attgcatgcc ctttatttat tgaccgaaac cgcggaatcg tatttcggaa aacggggctt
    16921 tcagcagatg gaccgggacc tgacaccgga agaattaaaa gccagcgccg agttcaagtc
    16981 cttatgcccg gcctccgcga aactaatggt taagtggctg aattgatgaa ttgttgaatt
    17041 gatgaattga cttgcgatta cttactaact tactaactta ctaacttact aacttgctaa
    17101 cttgctaact gactaactaa ccaacttttc tttacattag tcctttaaac caaaactacg
    17161 gatgcagagt cttcaaatca aagaaagtcc cactgaatac gatgtcgtca ttgtcggttc
    17221 gggtgccgga ggtggaatgg ccacttatat cctggggcaa gccggtctta aaatatgtct
    17281 tctcgaggcc ggcccgatgt atgatcccaa aaaggacgtt acccagttta aatggaattg
    17341 ggaatcgccc aggcgcggag caagcaccaa attccgcaat ttcggggatt acgatgcctg
    17401 ttatggagga tgggaattgg acggggagcc ctatacccag aaacccggct cgcgttttga
    17461 ctggttccgg gccaggatga tgggggggcg taccaatcat tggggcagga tctccctgcg
    17521 attcggaccc cgggacttta aacaccggag tctagacggc ctgggtgaag actggccgat
    17581 cgggtatgag gacctcaagc cctattacga ccgggtggac cagatgattg gggtattcgg
    17641 aaccaacgaa ggcctggtga atgatccgga tggttatttt ctgccaccgc ccaaaccccg
    17701 gttgcacgag ctttatatca aaaacggggc catgaaggcc ggggtcaatg tcatcccttc
    17761 gcgcctgtcc atgctcacca aaaaaataaa tgatgagcgg ggggtctgtg tttattgtgg
    17821 ccaatgcggc agggcctgcc agtattacgc cgatttttcg gcttcctcct gcctggtgat
    17881 cccggcgctt aaaaataaga atgtggaatt ggtctgcgat gccatggccc gtgaagtgac
    17941 caccaacgcg gaagggctgg ccacgggcgt ttcctatgtg gaccgcaaca cccttcagga
    18001 ataccatatc aaggcaaaag ccgtgatcct ggcagccagc gcctgcgaat cggcccgcct
    18061 cctgctcaat tcaaaatctt cagcccaccc caacggcctg ggcaattcca gcaatatcgt
    18121 cggcaaatac ctgcacgaca gcaccggcaa ggatatggga ggggtcttgc caagactgat
    18181 ggaccacgaa cggtataacg aagacggggt cggcggcgcg catatttatt ccccctggtg
    18241 gctggacaac aaaaaactcg actttccccg cggctaccat atcgaatact ggggcggcat
    18301 ggggatgccc ggttacgggt tcatgggggg aatcgaaaac ctcaacggca aattccccgt
    18361 taagggcaag accaaggaag ccggggggta cggcgcctcg ctcaaggaag actaccgggc
    18421 cttctacggc gccatggtgg gtatgtcggg ccggggagag gccatcgcgc gcgaggacaa
    18481 ttattgcgag atcgatccca atgtggtgga taagtatggc attccggtgc tccgcttcca
    18541 ttacaaatgg tccgattacg aaatcaaaca ggccaaacat atgcaggata ccttccagga
    18601 aatcctcacc cagatgggcg ccaccatcac tttcggggtc gattccaaag aaaacaactg
    18661 gggactcgcc gcccccggcc ggatcatcca tgaagtggga acggtgcgca tgcacgacga
    18721 tcctaaaaag ggccccttga ataaaaactg ccaggcccat gattgtaaaa acctttttgt
    18781 agccgatgcc ggtccctttg tctcccaggc ggataaaaac ccgacctgga ccatcatggc
    18841 cctgtccatg cggacctccg agtatatcct ggatcagatg aaaaaacgcg aaatctagtt
    18901 atgttgaatt gtaaaattgt ccaattgtcg aattgtagct atttctaact tcaatcgata
    18961 tctattaacc ccatcgtatt aagcacaaaa aaatcctcca aatcctccaa atcctccaaa
    19021 tcctccaaat cctccaaatc ctataaatcc aacaaatcct atcaatccta ttaatcctat
    19081 taatcctact atgaaaagac gcgaatctct caaaaccatc gccctgggta cggtcaccgc
    19141 cgccgccctg ttaaaatcct gcaaagcgga ggaaccgaaa ccaggggata cctccgcctc
    19201 tcctgccgcg cccggcaaga ccccaccgga aggccccgca cgccggccgg aagagatcgc
    19261 ccattataag gaagtcatct ccaaaacctt tttcaccacg gacgaaatgg cctctatcgc
    19321 catcctggcc gatatcatca ttcctaaaga tgaggtgagc ggcagcgcca ccgaagctgg
    19381 agttccggac ttcatcgaat tcatcgtgaa agaccagccc cattaccagg ttcctatgcg
    19441 gggcggactg cgctggatgg acctgcaatg cctacagcgc tttcagaaac ctttcaagga
    19501 ttgcagcgcc gcccagcagc ttgaagtggt tgacctcgtg gcttatccca aaaaggcaag
    19561 gccggaacac tcccaggggg tcagtttttt caataccatg cgaaacctga cggccaccgg
    19621 tttttatacc tcagccatgg gggtgaagga tattggattc aaaggcaata cccccaatca
    19681 atgggccggg gtgccggaag acgtgctcaa ggcgcatggg ctggcttata cagagaagga
    19741 gttgaaggag tgtatctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt
    19861 attcaatacc cattaccacg gcgaccactc ggggggcaat gaaaactttg ccaatatggg
    19921 cgcaaccatc atcgcccacc agaatgtacg ggcccgtctt tcagctgacc agcgcatcat
    19981 caatttttca caaccccaga aagccgctcc ggaagccgcc tggcccgtca ttactttcgg
    20041 cgatgagctc aatgtctttc tcaacggtga ggaagtgatg atcttccatg tggaacatgc
    20101 ccatacggac ggagatgccc tctattacct gccccggagc aacgtcctgc acaccggaga
    20161 ctgttttatg aaagaccgct ttccttttat cgatcgcaat tccgggggat ctgtggacgg
    20221 ctggctcgct gccgcatcca ggaccctgac cctgattgac gaggagacca tcatcatacc
    20281 cggacacggg gatttggcca ataaaaagga ttaccgcgac gcccgcgatg ccgtcatgac
    20341 cttgcgggac gccgtaaaag ggaagatcag ggaaggcctc gaccttgatc agatcaaggc
    20401 tttaaacctg ggaaaaccct ttgataccac ttttggcagc ggcttcatta aaagcgacgc
    20461 cctggtaacg acgatctata aggatgagat gggggagaaa tagaataggg ataagtcacc
    20521 gtggatagtg gttagtgggt ggtggttgga gatactctac catttctccc gtttcccgtc
    20581 tcctttcctc ctacatccac agagctgcct taagaatcgt ggcaacggac aaagcatcgc
    20641 ggatttcccc ttttttcacc atttcaacgg cctcgtgaaa atggactttc cggatttgga
    20701 ttttttcatc ttcttcgggg taagcggttc cttccgtaag tccggtggcc aggtaggaaa
    20761 cggaaacttc gtcggagacg gaattggata attgcatttc gaggatgttt tcgtaccggg
    20821 tggcccgcag tccggtttct tcagcaagtt ccctttgggc tgcggccagg gtcgattcac
    20881 cctctttgca acctccttcg gggatttccc attcatagct gtccagggga tagcggtatt
    20941 gccccaccag ccaggtatag ccctggtcat ccaggggaag aacggctacg gcgatgtttt
    21001 taaaatggat ctttccgtag atgcccgggt ttccagacgg gttgatcact tcgtgttctt
    21061 cgacccggat ccaggggttt tcatacatga ttttttcggt cagggtggtc caggggttgg
    21121 caggatcaga catgattcaa aactacaaag gctctgccaa caatatttta tttttatccc
    21181 atggatctaa agctgctggc tcatcttccc gagttccacg tgcacccgga gagcaaactt
    21241 attgtccgtt ttcaggattg tgaccctttc cgccacctta accaatcccg ctacctggat
    21301 tattttgtca atgcccggga ggaccagttg agagaacatt atggccttga catttaccgc
    21361 ttcgccatgg aaaatggcct ggcctggatg gtggtgcgca accagatcgc ctacctgcgc
    21421 caggtcatgg tcaatgaaat cgtgaccatc cagacccatg tgatcgaatt tgaccgccaa
    21481 aacctcaccg tcgaaatgat catgtgggat gaaaacaaaa tcaaacccaa ggctttgctc
    21541 tggggcacct ttgcttttgt caacctgctt gaaggacgca gggccgaaca cccggatgaa
    21601 atgatggagt tctacaccaa ggtccattgg aacggagcgc atgggcagag cttcgatcag
    21661 cggattcaag ccctctatgc cataggaaag gagaaaaaac cggttcagcc agggtaaatg
    21721 gcattcaaaa tctttttcac gctgatcgcg ttgttataaa tggaatcgaa aaggttttgc
    21781 cttttgtccg attcttccgg gagataggct tgttggctca gggttttgat tttctctatc
    21841 cactccgggg cagagggccc ggccaggtga aggcatccgg cgagttcggg attcgcttct
    21901 ccgatcaggg tattggaaag gatatgtctg ccgaaggcca gggaatccag gattttcatt
    21961 ttaaatccgt tgttttgact gaaaggcaac aaatgaatat gtgccttttc caggatttcc
    22021 cgcatttgaa cagttgatgg agagtcgatc agggtgattt gctgacgacc ctccatctcc
    22081 ctttttaatt ttataccggg acggaatccc gcaatgacca ggggataatc cagttttgaa
    22141 aagacctggc ggataagaaa aacggcggct tcctcattct cccggatact cagatcaccg
    22201 tggtaaagcg catattcccc aaaaccggct ttgccacccg cctcgcggtg tggatgaaaa
    22261 ggggggatcc agacggaagg aatatttcgt atggaaaggt aatgatgttc tgcctgggag
    22321 atggcaaaga ccccggttgc cttgggcaac cctttttcct caagggattt gctcctcgag
    22381 gattcgaagc ggaaataccc ctttcttaaa agatgggtct ccgtgcgacc caggtggcca
    22441 taataaagag attcaatatt atgcatcctt ataaattttt tccggtccgc cagacgagga
    22501 tcctcaaaat gataaaggca atgcaggcct tcaaacagga tgggatgctt atcctggcaa
    22561 agcatatcca aaagttgagc cgacctcctc gattgtacaa ataagggctg ccgcaagcct
    22621 gtgattttcc acatggcttt ccttggatac aggtatatgg tgcggcaata ttttgtcagt
    22681 tcatccggca aactgaattt tttataaaag gcgtggagaa tgacctccac tcccgcttct
    22741 ttcaggctcc ggatccggtg gtaaatgccg ctcacatttc cccgcaaggg aggataagga
    22801 atatccaggg agacgatatg gaggcatgga gaatccatgt gggtgcaatg atagcgtatt
    22861 atcaggtaag cggccccgga gaatagtatc cggaggccta ttcttcagtc tgccggggaa
    22921 ggaactcttg aattgaatag tcgatagtaa cggatcttgc caatcttggc gtttttgcgc
    22981 gaaaatcata gcgtcatgcc gtctttgcga caacaaaatc gtcggatccc agacctccca
    23041 acagttcctt acctttgaat catgaggtct acattcagat ttataatccg tatttccggg
    23101 atggtcttga tcctggtttc agccgctgtt tattccgcaa tcgcccaggc caggtttgcc
    23161 tccatccagg aagccctgtt cagtgccggt aaaatgaatg gccagggtgg gccgccgaac
    23221 gtcaactgga tccgggatgg gaaccaattt tcctacatca aaggggccgg gaagataaac
    23281 acctataatc cttctacgga taccgatgaa gaagtcttca atgccggatc gctgaaattt
    23341 ccgggtacgg ataaggaatt tgcctatacg ggttttgaat ggaccgccga ttttaattac
    23401 ctgctgttcc aaaccaattt tcaaccggtg tggagaaaca gcgggaatgc cgattattat
    23461 ctcttttctt tcaaggataa aagcctgcgg ctggtagccc agggagccta taccgcccag
    23521 gtatcgccgg atggccggtc ggttgcctac gaacgcaacg gcaatctctt tgtcctggac
    23581 ctggccagta aaaaggaaaa acaactgacc cgggacggag ccgcttcggt gtacaacggc
    23641 cgttttggct gggtctatga agaagaattt ggtctcgtcc gggcctggga atggaatccc
    23701 gacagcaaat ccatcgctta ttggcggatc gatgaaaggg ccatccccat gtaccagtat
    23761 tcggacctca gcggaatgca tcctgaatac aaaaaaattc cgtacccgag ggtgggggac
    23821 ccggtgcccc tggcccgtat cgggattgtg gatataaaaa agggaaaaac cacctgggcc
    23881 aatattcccc ttcagggggg gtattttccc aggatttact ggacctccct gcccaatacc
    23941 ctggcggtgg caaggctgga ccgtaaacaa aaaaggcttg atctttttat ggtggactcc
    24001 aggaatggga ctgcccgaaa tattctcacc gaagattcca aaggatggat ggatgtgtac
    24061 aacttttttg cgggaatcct ccatcatttt ttcttccctc cccgggtgga agagttttac
    24121 tggatctcgg accgcagcgg ttaccagcac ttgtaccgct acgattacag cggcaaattg
    24181 atttcccagg tgacccaggg caactgggat gtgatgaccg tggaatccct ggatcccgcg
    24241 accggtctcc tttattatac cagtacggaa gcttcgggac tggaaaggca cctttattcc
    24301 attggcctgg atggttcctc caaaagaaaa cttacccttc agcccggccg gcaccagatc
    24361 cagtttgccc cgggcgggca gtattatata gatacctggt ccaataccac cacccccagg
    24421 caggttgaaa tccggaaagt caacggccag cttattaaga tctggcagga caatgccgcc
    24481 gtaaaaacct atgtttcttc gattgcctat gccccgcggg aactggaaac cgtcgtcact
    24541 tcagacggca ggaccctgga tatttacctg atcaagccag tgggcctcga tgtttccaaa
    24601 aaataccccc tggtcttgtc catctatgga ggaccggggg cacaaagtgt atataactcc
    24661 tgggccgcca atccctatga gcagtatctc gcccagcagg gttatgccat cgcttccgtg
    24721 aataaccggg gcagcacggg ttatggtcgt gattttatgg aatcggtcta tgagcagatc
    24781 gggaaactgg aaacggaaga ctttgtagcc accgccgacg ccctggcacg aaaatattcc
    24841 tggctcgacc gccagcggat ggccattcag ggccatagtt acggcggctt caccacctgt
    24901 tataccctga ccaaacaccc cggtgcgttc agggtgggta ttgccggcgc cccggtagtc
    24961 aaccaaaaat tatatgataa tatctattcc gaacggtata tggggatcct cccggagaat
    25021 gaccagaaat acgaagacca tgccccgttg aattttgtga aaaacctcag cgaccccttg
    25081 ttgatcgctc acgccctgga ggacgacaat gtccatgtca tcaatacttt tcagtttgta
    25141 aaagccgcca ccgacgcggg caaggatgtt gatctgagga tctatcctcc cggccaccac
    25201 ggggtagcct acagcgcggt gagccaaatc cgtttgtggc agac
//
LOCUS       DAOQXT010000069        24959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2321_length_24959_cov_6.098619, whole genome shotgun sequence.
ACCESSION   DAOQXT010000069 DAOQXT010000000
VERSION     DAOQXT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24959
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_24959_cov_6.098619"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="PKM27_12425"
     CDS             <1..1731
                     /locus_tag="PKM27_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNR08117.1"
                     /translation="YQLSKSTLIAKINELRADDKIEGIHDVRDESARDELKVRVVVSL
                     KKDAIAQIVLNQLYKYTPLQSSFGVNNIVLVNGRPRLLNLKQLIQEFIKFRLEVIIRR
                     TSYLLRKAEERSHVLEGLLIAIDHLDEVIKLIRESETPDEARDQLMLRFSLSEIQAKA
                     ILALTLRQLTGLERAKLKEEYEELQKLIQRYREILANESVQKGIIKEELQEMSERYGD
                     ERRTEITINESEINIEDIIPNEEVVITITHLGYIKRTKVTEYRSQGRGGRGSKGSQTR
                     DEDFVEHLFTATTHAYLLLFTQQGKCYWLKTYEIPEAPKTGTGRAIQNIVSLPPDDKV
                     KAYISIKDLSDEEFINNHYIVFCTRKGIIKKTLVEEFSRPRTNGINAITINEGDQLIE
                     ARLTNGRNEILIANKQGRAIRFNESKVRAMGRNAAGVTGVELDEDNDEVMGMICVDPS
                     DKTTSVLTVSELGSGKRSDLEEYRVTNRGGKGVKTLNITEKTGTVVSIKAVSDHHDLM
                     ITTTHGIMIRLDMADLRVMGRNTQGVKLIRLDEGDEIADVTVIVKESDEVKNGNGDSG
                     HPVDEAGGTP"
     gene            1823..3184
                     /locus_tag="PKM27_12430"
     CDS             1823..3184
                     /locus_tag="PKM27_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08118.1"
                     /translation="MKKLLLFSVLSLLGISLSFAQEDPEKLVKKALKSFNAFNLEPNT
                     KGASLDEAKATIDEVFKSEAMNSNVGALLAKGQIYGGYILKQQTQKLLNPAYNEKLDF
                     PAAFTAYEAYSKALELGQKKFEKSDALKGLQGLTGELNNTGNELYNEGNFEGAFNHFL
                     ASLMIHEKMKANAQVSALDKPEDYNNQLFILAASAVKAKKFAEAKLYNDKLIAANYQD
                     GGIYENNYEILMEAGDTKGAEMALEEGRKKMPDDVGLMFKEINHFIRLGRLDLLVDRL
                     KSAIEKEPKNTSLYTTLGNVYDNLYQKDSIVMEKDAAGNYTVMASVNSNINFKSAEKY
                     FTEATQLDPKNGDAHYGLGAFYYNVAARMTAILNKLADDYSKEGSKKYEAIKVEVFTM
                     FDKSLPSFKMAESINPNDRNTLIALKEIYARENKFDLSNEFKKRLEIIEAGGSNPSSY
                     HKN"
     gene            3286..3729
                     /locus_tag="PKM27_12435"
     CDS             3286..3729
                     /locus_tag="PKM27_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08119.1"
                     /translation="MEYDTPSPVREAGLSDLPKIIPALKILRPHLSDQDFYTMIPVLF
                     EEGYRIIFIGDDRLAYSVAGYRTLNFLYSGKTLYIDDLVTLPDHLKKGYATQLLAWLK
                     SYARNNGFDHFSLDSGFQRKTAHRLYLNEGLEMEGFHFGRKVSDL"
     gene            complement(3826..4614)
                     /locus_tag="PKM27_12440"
     CDS             complement(3826..4614)
                     /locus_tag="PKM27_12440"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR08120.1"
                     /translation="MLKNLTLFVLFLWLAPGAVLSAQDQSVYVKKEFISSKGTHLPYR
                     LLYPDVPAKGKLPLVLVLHGGGERGNDNEKQLTHGAGLFTEPANRKNFPAYVLFPQCP
                     GDSYWASATIDRGTNPFKIEIDYSKPVPKGLEATMELVKHLAKTEKIDKKRIYITGLS
                     MGAMGTFEAVHRFPKTFAAALPICGIADVEAYNARAKKVPFWIFHGDADAVVSVSGSR
                     DIVAKLRSLGYTVTYTEYPGVNHNIWDHAFKEDNFLSWMFSKRR"
     gene            4756..5931
                     /gene="mqnE"
                     /locus_tag="PKM27_12445"
     CDS             4756..5931
                     /gene="mqnE"
                     /locus_tag="PKM27_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNR08121.1"
                     /translation="MHDAIDQLVRTDQDAAIWQKVKEGRRIGDDEALYLYDQADLGFT
                     AALANWLREQKHQHRTYFNRNIHIEPTNVCIYTCKFCSYSRLIKQRSEGWEYTKEQML
                     EILSGQKQKEITEVHIVGGVLPQYDLSFYADLFREIKSLYPALHIKSLTPVEMHYIFK
                     KAKKSVEEGLMILKEAGLDSLPGGGAEIFDEEIRQQICADKCGTEDWLKIHKAWHQLG
                     GRSNATILYGHIESYAHRVDHMRRLRDLQDQTGGFQTFIPLKFRNKDNEMSHIPEVTT
                     LEDMKNYAVSRLYLDNFDHIKSYWPMLGREMAQLSLSFGVDDLDGTIDDTTKIYSMAG
                     SEEQNPAMTTEELVLLIQRAGRLPVERDTLYRILKEYTEPLVAGPSRPYYALPVVNH"
     gene            5934..6701
                     /locus_tag="PKM27_12450"
     CDS             5934..6701
                     /locus_tag="PKM27_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNR08122.1"
                     /translation="MSVPKVAIVNYLNTLPFLHGLRSSGFVHEMELIEVMPSLCARYF
                     TEQKVDISLVPVGALTSFPDALFSPYGIACDGPVASVCLLSEVPLHQVNKVLLDYQSR
                     TSVLLVRILLEKYWKLFPRFQAAGEGFENAIHGNTAGLVIGDRALLMSPRYPYVFDLG
                     QAWKDYTGLPFVFAVWMYNRLPNPDFIRRFNKALQFGLDHIDAAIHNNSSYDAPFLKQ
                     YFNRNIQYLLEPEYLKGMRLFFHEKQSLEASLTSTLG"
     gene            complement(6723..7406)
                     /locus_tag="PKM27_12455"
     CDS             complement(6723..7406)
                     /locus_tag="PKM27_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08123.1"
                     /translation="MKFTRSVPVIRGWLIVGYLVYMGVYGFGQVGIKAVGTFAKNRIA
                     EITSQERLFSSRFGLGADYWFRLKKYRLEFLPSIHLQYGRERFNITDQVVGDLSWSSL
                     DFAPAIQFYPLDFFSDCQCPTFSKQGKFLKKGLFLSLAPGIGYHVLNSKNLNESSGDW
                     LLFGRAGLGLDIGLSDLLTLSPAVHYQLSQNMNWSRYFLSSNSASLDINSGLFLSLRL
                     GLRMDKNRY"
     gene            7458..8768
                     /gene="rimO"
                     /locus_tag="PKM27_12460"
     CDS             7458..8768
                     /gene="rimO"
                     /locus_tag="PKM27_12460"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNR08124.1"
                     /translation="MRLKGQKKNKIHVITLGCSKNTVDSENLITQLKHNQFEVEHDAD
                     QSDAKVIVVNTCGFIERAKKESIDTILEYAEAKNEGRVDRLIVTGCLSQRYKDQLKTE
                     IPEVDAWFGTMDLPALLANLQADYKKELLGERMISTPGHYAYLKISEGCNRTCSFCAI
                     PLMRGGHVSRPMESLVHEAEFLAARGVKELILIAQELTYYGLDLYKKRKLPELLAALG
                     QVEGIEWIRLHYAYPSKFPLEILDEMAVNPKICKYLDLPLQHASDAVLKAMRRQITKS
                     ETIDLIGKIRDKIPGIALRTTLLVGFPNETEKEFDELCDFITAMQFDRLGVFTYSPEE
                     DTEAYSLQDLVPEEIKTARADRIMEIQRDISRMKNESKIGRILPVMIDRKEKKHWVGR
                     SEADSPEVDQEIHLAPTKESLQPGNLVSIRITGAEDYDLMGEIV"
     gene            8777..9235
                     /locus_tag="PKM27_12465"
     CDS             8777..9235
                     /locus_tag="PKM27_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08125.1"
                     /translation="MLLTDTGFRHWAARPAVQAGFVFLVMLAANLLVKLLAFGGIEFE
                     SRIPWTLNIAFLLLFSIYNVVLGLLYSTGYRYWTYSISCFIGLAFLSAWSAKWLSHLS
                     MDEAGSFRWLYLVFFMVYFLIMMITLAIRKIVSLAEQDDHRFDEVKPKDN"
     gene            9254..9751
                     /gene="msrB"
                     /locus_tag="PKM27_12470"
     CDS             9254..9751
                     /gene="msrB"
                     /locus_tag="PKM27_12470"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNR08126.1"
                     /translation="MKIILFLFTLYTACNQLNQQPMPVTESVTAAASDTIKKMVKSEE
                     EWKKELSPMEFLVLRQKGTERAFSGDLWDHHEKGVYTCRGCQLPLFSSDTKFESGTGW
                     PSFYQPISRESLADYADNTHGMSRTEVVCARCGGHLGHVFDDGPRPTGMRYCINSVSL
                     DFQKR"
     gene            9732..10514
                     /locus_tag="PKM27_12475"
     CDS             9732..10514
                     /locus_tag="PKM27_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08127.1"
                     /translation="MTFRKDNRVPLKAIGFLGYFFLAWCTNAQSSFSGTFEGKINGDP
                     ARMELVQQGDGVTGKYHETGNTFDIRAKAEGKDCRGSLLISGTEIALATFNFRISETG
                     LRLTILLPDQSRVEAEFVRLAEPSKQPAGPFNHEKTLTAAQDGLPRDPAVVGRWMKED
                     ILNSGTGTSAASLVTVYYLSLYEDGRFIQEKARSAGGTDWSSIANRTTDLTGHWYTQN
                     QVMYVRPAGEKEYLRLHPYLFHNGALVFKTEDGKYLIWNKQN"
     gene            complement(10532..11722)
                     /gene="kbl"
                     /locus_tag="PKM27_12480"
     CDS             complement(10532..11722)
                     /gene="kbl"
                     /locus_tag="PKM27_12480"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNR08128.1"
                     /translation="MNQSVLTSLRDELDQLKANNLYKKERTITGPQGAVIQTLEAGEV
                     LNFCANNYLGLSSHPEVIRAAKETIDSHGYGMSSVRFICGTQDIHKKLEGALSEFLGM
                     EDTILYAAAFDANGGVFEPLLQEEDVVISDELNHASIIDGIRLCKAGRKRFLHNDMQD
                     LEEKLRESGSARRRMIVTDGVFSMDGTIARLDLICNLAEKYDAMVMIDECHATGFMGK
                     TGRGTHEYRDVMGRIDIITGTLGKALGGASGGFTSSRREVIEILRQRSRPYLFSNTLA
                     PSITGASLRVIQLLSESTALRDKLEKNTAWFRQAIHEAGFDILPGSHPIVPIMLYDAA
                     LAQEFAAKLLLKGIYVIGFFFPVVPKGKARIRVQISAAHEPVHLEKAVQAFTEVGREL
                     GVIK"
     gene            complement(11725..14154)
                     /locus_tag="PKM27_12485"
     CDS             complement(11725..14154)
                     /locus_tag="PKM27_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR08129.1"
                     /translation="MKRLFFFSLLLLCLKLQSQDQTNPYQTPSADLVDLVNAPPSPSV
                     LFTRNGGWMIIQQRQAMMDIADLAQPELRIGGLRINPATFSQSRIPFIVSLKLKESRS
                     GREYDLEGLPENPKFSFVRLSPDEQKLAFIHQSAQGNELWVADLKTRKTKRWSNVFIN
                     SSTGNPYQWVHGAEILAKAVREGKQPPRENPVPPGPVIQETEGVAAPAATFQDLLKNP
                     FDEALFEYYCSVDLVRISAEGTLPVSRDLIAGRFDLSPDKKYILLHRIKKPFSYLVPY
                     SRFATQIEILDLSGKTVRILADNPPDEVRPRGFDAASRLPRNFQWRDDLPATICWVQA
                     LDEGDPKKKVEFRDALQQLEAPFSGSPLTLYQSGMRLQDVYWSDKYLAFAREGLFATR
                     QILIKKFNPFLPDLAPDTLFAYSSQDAYNDPGEPVLAENQYHRQVVMTNPAKNLIYTI
                     GKGASPDGDMPFLSSFDLKSKKQEILWRCKAPYYEYPVHVIDLQEGSFITSRESQTEN
                     PNYYLRNYKGLNPTALTSFPNPQPQLANLKKEKIEYQRADGLNLTAVLYTPKGYDKTK
                     SGPLPVLMWAYPREYKSASDAAQVRGSKYSFTRITYGSPVFWALRGYAVMDQTEMPIV
                     GSGDEEPNDTYIDQLVKNAEAAVEKISSMGIGDKNRIGVGGHSYGAFMTANLLAHSDL
                     FAAGIARSGAYNRTLTPFGFQNEQRSFWEAPEVYHAMSPFSHADKIKEPILLIHGEAD
                     NNTGTFPMQSERLFHAIKGNGGTARLVMLPHESHGYAAKESIYHMLYEMDQWLEKNVK
                     NAGVKSTKPKS"
     gene            complement(14202..15146)
                     /locus_tag="PKM27_12490"
     CDS             complement(14202..15146)
                     /locus_tag="PKM27_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR08130.1"
                     /translation="MKILITGANGQIGTALAAALRDRYGRDKVLCTDIYKPKEDHGPF
                     EMLDILNVQRMSEIIEDHHITQVYHLAAILSANGEWNPKKTWNVNLNGQLSILELAHQ
                     YQMDKVFFPSTIAIFGSTTPKSNTPQHTAAEPSTVYGMSKLAGELWCQYYHSRYEVDV
                     RSLRYPGIIGWQSAPGGGTTDYAVEIFHAAIRQEAYTSYLSEDTFLPMLYMDDAIRAT
                     IELMEAPAGQISIRTSYNLSGLSVSPGTLYREIKKHLPDFTIRYEEDFRQKIAESWTE
                     SIDDSVARKDWGWRETFDLSRMTQDMLAHLGAPVRPET"
     gene            complement(15204..15650)
                     /locus_tag="PKM27_12495"
     CDS             complement(15204..15650)
                     /locus_tag="PKM27_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNR08131.1"
                     /translation="MILNLLSDTTPLKFSGKNGQRTVLDPIRKKWLALTPEELVRQLF
                     VVYLIRKLEYPASRIAVEKQIRMDRTIKRFDLVVYSSALAPFLLIECKSPEVMLDEQV
                     VSQVVRYNLAVKADFLCIVNGLECRVFQKSEDGRWREVDFLPAYIP"
     gene            complement(15653..16279)
                     /locus_tag="PKM27_12500"
     CDS             complement(15653..16279)
                     /locus_tag="PKM27_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08132.1"
                     /translation="MTKITCMPPALQFSLANCKAMLWSILFTEFLIFSTACRKAKGPP
                     PAYETVSLEGSDQKKVFKRDSLGNLLVEGVIFQGKRNGSWVTYHPSGQVETITNYLED
                     RKNGLYLRFGQNNQIEEQASYMDDLLEGTFIRYQFGHLDERIEYKAGKKNGRAIKYYL
                     RGGMQREMEFKDDVQDGFYRFYGEDGTKQIEEVYRAGKKVSGGIILSK"
     gene            complement(16276..16893)
                     /locus_tag="PKM27_12505"
     CDS             complement(16276..16893)
                     /locus_tag="PKM27_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08133.1"
                     /translation="MTRMLNRQAFGDPANAGNWPTEKEARDLLENWVPNEKLRNHMFQ
                     VGWLLRCWAKEKEGLDEHHQWKWQVTGWLHDADWEKWPETHCQKIVEELEQRQTDPEV
                     INAIAAHGPAHFGVEPKTRLDKMLYAFDELSGFVHAYSLMRPGGYQGMETKGVLKRLK
                     DKTFAAQVSREDIRDAAERIPMPVEELIDFVISHQADTPQIKSRP"
     gene            complement(17026..20325)
                     /locus_tag="PKM27_12510"
     CDS             complement(17026..20325)
                     /locus_tag="PKM27_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNR08134.1"
                     /translation="MSDFHFIPKKVFITLFFSILAGWSFGQGVTSANIAGLITDSEGE
                     ALIGATVLALHEPSGSRYGTSTREDGRFALPNVRIGGPYTITVSYVGYKDYQEKNITL
                     SLGQTLQFNVSLADESAVIQEIIVTSDKGSVLSSERTGAGTNIKKELLEAMPTISRGL
                     NDFLRYVPQSRSSSVASTTGAGTSFAGQDSRFNNLTIDGTIFNNSFGLASVPGGQTSS
                     TPISLDAIEEIQVSLAPYDVRQGGFTGAGVNAVTRSGTNKTQGSVFYNRRNESLTGKK
                     AAGTSIVRSDFDVQQFGGRLGGAIIKNKLFYFINFEQEKRTDPSSTFIANRGTTGNNV
                     TRVLASDLDQLSTFLKSKFSYDPGLYENFPLRTESHKGLAKIDYNINDNNRLSVRFNY
                     LKSLRDVTASNSGVFSGNRSNNLFALNFQNSNYIINNDLYSGIVELNSIIGTKFANNM
                     QLGFTANRDYRSSGGGVFPLVDILKDGTTYTTFGYEPFTPNNILNTDTWQFKDDLTFY
                     RGQHTITAGVNFESFKFDNTFTPTYYGQYIFNSLDDFYAAANGNADITLARYVLTYSA
                     LPGKALPTATTRAYQPGVYLQDDIALFKDRLKLTVGLRADMPFFGNTARNNAEVETFT
                     FKDAEGSPLKLSTSKLPDAKVLFSPRVGFNWDVNGDRTFQVRGGSGIFSGRPAFVWIS
                     NQVGNNGVLTGSLSVNSTKAYPFSPDVTAHIPDNATSPPTYNIAVTDPNFKFPQLLRT
                     NIGVDMKLPLGMVGTLEGIFSKNLNQVYYINANLEPATGAYSLPDVRPTYPGLGLSGT
                     TQANALRINDKITDAILLTNSSKGYTYSLTAQIQKQFARNFFFNGAYNFAEAKDLMTA
                     GSIAFSSWRDNPSRLGNNRQELAFSDNDQRHRIIAALYYKFNYGWGGTGINLGFQTGN
                     QGRVTYTVNGDVNGDGLTTNDLLFIHKNPNDIQFEQYTVGSGANARTITVDAQKAAYD
                     KFLSSVGYLNDNRGSYAERNGFLLPWLTTFDVSLTQDINVNIAGKKNTLQFRADLVNA
                     GNMINDQWGVGTITTTTNPLQFRSRNAATNQPVYRFTEISNKFPETGTRYGSRLTDVW
                     QIQLGARYIFN"
     gene            20515..21471
                     /locus_tag="PKM27_12515"
     CDS             20515..21471
                     /locus_tag="PKM27_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08135.1"
                     /translation="MTPLQEEIKRLLEEHQAVKKSESRLGELSSEINQARQDLARLEK
                     MIEKEYQDMEKLEKIGMKALFYKVLGNQQEQVEKEKQEYLQVSLKYDQVRKNLELLEF
                     ENNILRKKISAGPEIEKRLNELSLLQEKELMNAESAGTSELRKLNGQIETLQRLKIDV
                     DEAAKAGEKALECIDGVVAGLQQAHNWGQWDMSGRNPMSNHLKYTAIDKAKDWAYEAN
                     HQLKRFEKELIDVYRNIPQDFQLQLDNFNRFIDVFFDNLITDWIIQQKIYNALNNTLA
                     TRDKVVRMLKTLDAETPRINRNIEELELAKKQFVLNFSKGLQ"
     gene            complement(21544..22242)
                     /locus_tag="PKM27_12520"
     CDS             complement(21544..22242)
                     /locus_tag="PKM27_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR08136.1"
                     /translation="MAKVKSSVKKADEKLLDLVEVQQKTKSFFEQHQQKISIIGGALV
                     LGLAGYLAYLFLYLQPRQERAVEQMMVAQQQFEKDSFRLALTNPGGGYSGFLDIIDSY
                     KGTKTSNLAKYYAGISYLHLGQYDAAIDYLEDFSPKDEVTTITRAGNLGDAWSEKKDF
                     NKAMSYYKQAIAAGKNELLTPYYIKKAGLLYLYQKDNANALSQFNELKTQFPLSPEAS
                     DIDKYIVKAGGTAE"
     gene            complement(22349..23371)
                     /gene="pdhA"
                     /locus_tag="PKM27_12525"
     CDS             complement(22349..23371)
                     /gene="pdhA"
                     /locus_tag="PKM27_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNR08137.1"
                     /translation="MQVIEKPLAGTEVRYDKATYMKWYETMLRVRRFEEKTLLMYSQQ
                     KIRGFCHVYIGQEAIAGGLATAIRPEDAVITGYRQHGIAITRGLTASSCMAELFGKID
                     GCVKGKGGSMHFFSEKHRFFGGNGIVGAQIGIGTGIAFAEKYKGTDNICVTMFGDGAA
                     RQGILFESANMAMTWGLPVLYICENNGYAMGTSVSRSSNMVDIYKVGAAFNMPSKQVD
                     GMNVEKVHEALTEAAAYIRAGNGPYFLEIMTYRYKGHSVSDPATYRTKDELHHYMGMD
                     PISNLEEKLLSQSICTEQEILTIQDQLKAEMDEAVRFAEESPFPPAEELYTDNYVQPD
                     YPFLRD"
     gene            23510..24604
                     /gene="recF"
                     /locus_tag="PKM27_12530"
     CDS             23510..24604
                     /gene="recF"
                     /locus_tag="PKM27_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNR08138.1"
                     /translation="MILTQFKNYTHQAFSFHPGFNLVYGTNGSGKTNVLDAIHYLALG
                     KSHFVPQDKFSILQGESFFRIEGLFKQEEETVQWVLKFQPGTKRLEKDGKAYGRMQDH
                     IGEVGLVMIAPDDLDLIDGQSQARRKFIDLTLSQSDHIYLGHLATYKRLLDQRNALLK
                     SSPSPDLGFLQALNEKMTEPAHRIYSIRKKFVLDFLPLFYKYYHAISKAKENVEIRFQ
                     SDLDQADLLTLFNQGWTQDLYAQYTVRGCHKDDLVFLKDGEPLKYLGSQGQKKTFLVS
                     LFLAQSDYLSKTMDSPPIILLDDIFDKLDDERIRYLLHTIGNMSTRQVIITDTGKERL
                     SAVLKDLGAQVHFIHIRDGLVNTPLHDETT"
     gene            24588..24881
                     /locus_tag="PKM27_12535"
     CDS             24588..24881
                     /locus_tag="PKM27_12535"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNR08139.1"
                     /translation="MMKRHNDLPLSSVLKQWAGSEKLKPMLIQKNLEAHWEEWFGKLI
                     AKNTEKLSIRNRKLYIHIKSGPVKYELNLGRQNVMNLVNQKLGENYLEEVLVL"
BASE COUNT         6261 a         6249 c         6407 g         6042 t
ORIGIN      
        1 taccagttga gcaagtccac cctgatcgca aagatcaacg aactccgggc ggatgataaa
       61 attgaaggca ttcatgatgt ccgggatgaa tcggcgcggg acgaactcaa ggtacgtgtg
      121 gtcgtttcac ttaaaaaaga tgcgatcgcc cagatcgtac tgaatcaact gtataaatat
      181 acccccctgc agagttcctt cggggtcaac aacatcgtgt tggtcaatgg ccggccccgc
      241 ctgctcaacc tgaaacaatt gatccaggaa ttcatcaaat tccggctcga ggtgatcatc
      301 cggcgcacca gttatctcct tcgcaaagcc gaagagcggt cccacgtgct ggaaggcctc
      361 ctcatcgcca ttgaccacct ggatgaggtg atcaaactga tccgcgaatc ggaaaccccg
      421 gatgaggcca gggaccaact gatgcttcgt ttttcccttt ccgaaatcca ggccaaggcc
      481 atcctggccc tcaccctccg tcagctgacc ggcctggaaa gggcgaaact caaggaggaa
      541 tacgaagaac tgcaaaaact gattcagcgc taccgggaga tcctcgccaa cgagtcggtg
      601 cagaagggga tcatcaaaga ggagttgcag gagatgtccg agcgatatgg cgacgaacga
      661 aggaccgaga tcaccatcaa tgaaagcgaa atcaatattg aagacatcat ccccaacgag
      721 gaagtagtca tcaccatcac ccacctggga tatatcaaac ggaccaaagt gaccgaatac
      781 cgttcccagg gcaggggagg caggggttcc aaaggaagcc agacccggga cgaggatttt
      841 gtcgaacatt tattcaccgc caccacccat gcatatctcc tcctgtttac ccagcaaggc
      901 aagtgttact ggctgaagac ttatgagatc cctgaggcgc ccaaaacggg taccggcagg
      961 gctatccaga acatcgtttc cctgccgccc gacgacaaag tcaaggctta tatatccatc
     1021 aaggacctca gcgatgagga attcatcaat aatcattata tcgttttctg cacccgcaag
     1081 gggattatca aaaaaaccct cgtggaggaa ttttccaggc cgagaaccaa cgggatcaat
     1141 gccattacga tcaacgaggg cgaccagttg atcgaagccc gcctcaccaa tggaaggaat
     1201 gaaatcctga ttgccaataa gcagggccgc gccatccgtt tcaatgaatc caaagtgcgt
     1261 gccatgggcc ggaatgcagc cggggtaacc ggtgtggaac tggacgagga caacgacgaa
     1321 gtcatgggca tgatctgtgt ggacccttcg gataagacca cttcagtcct cacggtttcg
     1381 gaactgggca gcgggaaacg ttcagacctc gaagaatacc gggtgaccaa ccgcggtggc
     1441 aagggggtaa aaaccctgaa catcacggag aaaaccggca ccgtggtttc gatcaaagcg
     1501 gtatccgacc atcatgacct gatgatcacc accacccacg ggatcatgat acgcctggat
     1561 atggctgatc tccgggtcat gggcaggaac acccagggtg taaaacttat ccggctggac
     1621 gagggggatg agatcgcgga tgtcacagtg atcgtcaagg agagcgatga agttaaaaac
     1681 ggaaacggag attcggggca tccggttgac gaagccggcg gcactcctta actttgttaa
     1741 gctttttata aaatcattta actttttata aactttgtac tgatatttct ttcgtcatat
     1801 ttccaaaatt taaatgcaga aaatgaaaaa actcctttta ttctcggtgt tgagcctgct
     1861 cggtatttcc ctttcctttg cccaggaaga cccggaaaag ctggttaaaa aagcgctcaa
     1921 atcgttcaat gccttcaacc tggaacccaa taccaaaggg gcctccctgg acgaggcaaa
     1981 agccactata gatgaagttt tcaaatccga ggccatgaac agcaatgtcg gggcgctcct
     2041 ggccaaagga cagatttacg gcggttatat tttaaagcag cagacccaaa aactcctcaa
     2101 tccggcctat aatgaaaagt tggattttcc ggccgctttt acagcctatg aggcctactc
     2161 caaagcgctg gagctcggtc agaaaaaatt cgaaaaatcc gatgccctta aaggcctgca
     2221 ggggcttacc ggagaattga acaacaccgg caacgaacta tataacgaag gaaattttga
     2281 aggggctttt aaccattttc tcgcctcatt gatgattcac gaaaaaatga aagccaatgc
     2341 ccaggtcagc gccctggaca agccggaaga ctacaacaac cagttgttca tcctggccgc
     2401 ctccgccgtc aaagccaaaa aatttgcgga agccaaactg tacaatgata aactgatcgc
     2461 tgccaattac caggacgggg ggatctatga aaacaattat gaaatcctga tggaagcagg
     2521 ggataccaaa ggggcggaaa tggccctcga agaaggcaga aaaaaaatgc ccgatgacgt
     2581 aggtttgatg ttcaaggaaa tcaaccattt tatccgtttg ggccggttgg acctgctggt
     2641 ggaccggtta aaatcggcca tcgaaaaaga acccaaaaac acctccctgt acaccaccct
     2701 gggcaacgtg tatgacaatc tttatcagaa ggacagcatt gtaatggaaa aagatgccgc
     2761 cgggaattac accgtcatgg catccgtaaa ttccaacatt aattttaaaa gcgccgaaaa
     2821 gtattttacc gaagccaccc agctcgatcc gaaaaacggg gatgcccatt acggcctggg
     2881 cgccttttat tacaacgtgg ctgcccggat gaccgccatt ctcaacaagc ttgcggatga
     2941 ctattccaag gagggttcca aaaaatatga agccataaaa gtggaggtct ttaccatgtt
     3001 tgacaaaagt ctaccttcct ttaaaatggc tgaatccatc aatcccaatg accgcaatac
     3061 cctgatcgcc ctgaaagaaa tctatgcgcg ggaaaacaaa tttgatcttt ccaatgaatt
     3121 caaaaaaagg cttgaaatca ttgaagcggg aggatccaat ccttccagtt accacaaaaa
     3181 ttaaggtttc caaaaccagg ataaaaagcg ctggagctca actccggcgc tttttttttg
     3241 ccagtatagt tttttttacc ttgcagccct tcatgatctt attttatgga atacgatacg
     3301 cctagtccag tccgcgaagc gggcttatcc gacctcccca aaattattcc cgccttaaaa
     3361 attttgcggc cccatctcag cgatcaggat ttttatacca tgatcccggt tttatttgaa
     3421 gaaggatacc ggatcatttt tataggggat gaccgcctgg cctattcggt ggccggttat
     3481 cgcaccttga atttcttata cagcggcaag accctctaca tcgacgatct ggtgaccctg
     3541 ccggaccatt taaaaaaagg ctatgccacc caattgcttg cctggctgaa atcctatgcc
     3601 agaaacaacg gcttcgatca tttttctttg gactccgggt tccagcgcaa aaccgcccac
     3661 cggctttacc tgaatgaagg actggaaatg gagggctttc attttggaag gaaagtgagc
     3721 gacctctgat accagtgcgt ggggcggagg aagggagaaa aggagacagg aggaagggag
     3781 ggcgtctgac ctctgacctc tgacctctcg cctcttacct ctaacctacc tcctcttcga
     3841 aaacatccag ctcaggaaat tgtcctcctt aaaagcatgg tcccaaatgt tgtgattgac
     3901 gcccgggtat tccgtatagg ttacggtata acccagggac ctgagttttg caactatatc
     3961 ccgggagcct gaaacgctca cgacggcatc tgcatctcca tggaaaatcc agaaggggac
     4021 ttttttagcc ctggcattat aggcttcgac gtcggctatc ccgcagatgg gtagggcggc
     4081 tgcaaatgtc tttggaaagc ggtgcacggc ttcaaaggtg cccatggcgc ccattgagag
     4141 gccggtgata tagatcctct ttttatcaat cttctctgtt ttggccaggt gcttgaccag
     4201 ttccattgtg gcttccaggc ctttgggaac cggcttggag tagtctattt cgattttaaa
     4261 cggattggtg ccccggtcaa tggtggcgct ggcccagtag gagtcgccgg gacactgggg
     4321 gaatagcaca taagccggga aatttttccg gttggccggc tccgtaaaca agcccgcacc
     4381 gtgggtgagt tgtttttcat tgtcgttgcc tctttcccct ccgccgtgca ggaccaggac
     4441 cagggggagt ttgccttttg ccggaacatc cgggtagagt agacgataag gcaagtgggt
     4501 gccctttgag gaaataaact ccttctttac atagacggat tgatcctggg cggaaaggac
     4561 tgccccggga gctaaccaaa gaaaaaggac aaagagggtc aggtttttta acatgtttaa
     4621 acttacggag ttcctttgat tctttgcctc acccggcccg ggcatgcaaa aaaaacttgc
     4681 cccaaaaccc tttccggggc aagtccaagt tctaccatct atcccttacc tttgtacccg
     4741 aaggaaagaa tatatatgca tgatgccatt gaccaactgg tgcgtacaga tcaagatgct
     4801 gccatctggc aaaaagtaaa ggaaggccgc aggatagggg atgacgaggc tttatacctg
     4861 tatgaccagg cggacctggg ttttaccgcc gccctggcga attggttaag ggaacaaaaa
     4921 catcagcacc ggacttattt caaccggaat atccacattg agcccaccaa tgtctgcatc
     4981 tatacctgca aattctgctc ctattccaga ctgatcaaac aaagatcgga aggttgggaa
     5041 tatacgaagg agcaaatgct ggaaatcctt tccggtcaaa aacagaagga gatcactgag
     5101 gtgcatatcg taggcggggt cctgcctcaa tacgatcttt ccttttacgc ggatcttttc
     5161 cgcgagatca agtcccttta cccggccttg cacatcaaat cgctgacccc tgtggaaatg
     5221 cactatattt tcaaaaaagc aaaaaaaagc gttgaagaag ggctgatgat cttaaaagaa
     5281 gcgggactgg attccctgcc gggaggaggt gctgagatct ttgatgagga aatccgtcag
     5341 cagatctgcg cagataagtg cggcacggaa gattggctga aaatacataa ggcctggcac
     5401 caattgggtg gccggtcaaa cgcaaccata ttatatggcc acattgaatc ctatgcccac
     5461 cgggtggatc acatgcgaag gctgagggac ctccaggacc aaaccggagg ttttcagact
     5521 ttcataccgc tgaaattcag gaataaagac aacgagatgt cccatatacc cgaagttacg
     5581 accctggagg acatgaagaa ttatgcagtc agccggcttt acctggataa tttcgaccat
     5641 ataaaaagtt actggcccat gcttggaagg gaaatggccc agttgtccct gtccttcgga
     5701 gtcgatgatc tggatggcac cattgatgac acaaccaaaa tatattccat ggcgggttcc
     5761 gaagagcaga atccggccat gaccaccgaa gaactggtcc tattgattca acgggcaggc
     5821 cggcttcccg ttgaacggga taccctgtac cggatcctga aagaatacac ggagcctttg
     5881 gtcgccggtc cttcaaggcc ctattatgca ctgcccgtag tcaaccattg atgatgtccg
     5941 ttcccaaagt tgccatcgtc aattacctca acacgctgcc cttcctccat ggactgaggt
     6001 cttcgggttt tgtccatgaa atggagctca tcgaagtgat gccctccctg tgcgcgcggt
     6061 attttaccga acaaaaagtg gatatttccc tggtaccggt cggggctttg acgtccttcc
     6121 cggatgcttt attcagccct tacggtatcg cttgcgatgg cccggtggcc agcgtatgcc
     6181 tcttgtctga ggtgccgctg caccaggtga acaaggtcct gctggattac cagtccagga
     6241 cctccgtgct gctggtccgg atcctgcttg aaaaatattg gaagttgttc ccccgtttcc
     6301 aggcggccgg ggaaggattt gaaaatgcca tccatggaaa taccgcaggt ctggtcattg
     6361 gggaccgggc ccttctcatg agcccgcggt atccctatgt ctttgatctc ggacaggcct
     6421 ggaaggatta taccggactg ccttttgttt ttgcggtctg gatgtacaat cgactcccaa
     6481 atcccgattt tatccggaga tttaataaag cgctccagtt tggactggat catatcgacg
     6541 ccgccataca taacaattcc agttacgatg ctccgttcct caagcagtat tttaaccgga
     6601 acatccaata cctcctggaa ccggaatacc tgaagggcat gcggcttttt ttccatgaaa
     6661 aacaaagcct ggaagcaagt ctgacgtcca ccctgggctg acgaggcaag gacccgttta
     6721 aatcaatacc ggtttttatc cattctcaac ccgagacgga gggataggaa caaaccgctg
     6781 ttgatgtcca gggatgccga atttgagctt aggaaatagc ggctccagtt catgttttgg
     6841 ctgagctggt agtgcaccgc cgggctgagg gtcaacaagt ccgaaagtcc gatatccagg
     6901 ccgagacccg ccctgccgaa gagcagccaa tcgccagagg attcattcag gttttttgaa
     6961 tttaacacgt gataaccgat tccaggcgcc agactgagga acaaaccttt tttcaggaat
     7021 ttaccttgtt tggaaaaagt cgggcactgg caatcactga aaaaatcgag cggataaaac
     7081 tgaatggcgg gcgcgaagtc caggctggac caactgagat ctcccaccac ctggtctgta
     7141 atattaaagc gttcccggcc atactgaaga tggatggatg gaagaaattc gagcctgtat
     7201 tttttcagcc ggaaccaata atccgcccca aggccaaagc gggaggaaaa aagcctctcc
     7261 tggctggtga tctccgcaat gcggtttttt gcaaaagtgc ctaccgcctt tatccctacc
     7321 tggccaaatc catagacgcc catatacacc aggtacccca cgatcagcca accccttatc
     7381 acgggaacag aacgggtaaa tttcatggat aattgcaccg gcttaacctt acttttgccc
     7441 aaaattaggc gaagccggtg agattaaaag gacaaaaaaa gaataagatc catgtgatca
     7501 ccctcgggtg ctccaaaaat accgtggaca gcgaaaacct gatcacccaa ctgaaacaca
     7561 accaatttga agtagaacac gatgccgatc aaagcgacgc caaggtgatt gtcgtcaata
     7621 cctgcggttt cattgaacgg gccaaaaaag aatccatcga taccatcctc gaatacgcgg
     7681 aagccaaaaa tgagggcagg gtggaccggt tgattgtgac cggctgcctt tcccagcgtt
     7741 ataaggacca gttaaaaacg gaaatccctg aagtggacgc ctggtttggt accatggacc
     7801 ttcccgccct cctggccaac ctgcaggctg attataagaa agaactgctc ggggaacgca
     7861 tgatttccac acccggccat tacgcatacc ttaaaatctc ggaaggctgc aaccggacct
     7921 gcagtttctg tgccataccc cttatgcggg gcgggcatgt cagccggccg atggaaagcc
     7981 ttgtgcatga agcggaattc ctggcggccc gcggggtaaa ggaactgatc ctcatcgcac
     8041 aggaactgac gtattatggc ctggatcttt ataaaaagag aaaacttccg gaattgctcg
     8101 cggctctcgg ccaggtggaa ggcatagaat ggatccggct gcattacgct tatcccagta
     8161 aatttccact tgagatcctg gatgaaatgg ccgtcaatcc aaaaatctgc aaatacctgg
     8221 atctgcctct tcagcatgcc agcgacgcgg tcctgaaagc gatgcgaagg caaatcacga
     8281 aatcggaaac catcgacctg atcggcaaga tcagggataa aatacccggg atcgccctgc
     8341 gtacgaccct gttggtcggt tttcccaatg aaacagagaa ggagtttgac gaattgtgcg
     8401 attttatcac ggccatgcaa ttcgaccggc tgggggtttt tacttattcc cccgaggaag
     8461 atacggaagc atattccctg caggatttgg tcccggaaga aatcaaaacc gccagggccg
     8521 accgcatcat ggaaatccag cgggacatat cccggatgaa gaatgaatct aaaattggcc
     8581 ggatcctccc ggtcatgatc gaccgcaagg agaaaaaaca ctgggttggc cgttctgagg
     8641 cggacagccc cgaagtcgac caggaaatcc acctggcccc gacaaaagaa agcctccagc
     8701 ccggcaacct ggtctccata cgcatcacgg gcgccgaaga ctatgacctg atgggtgaaa
     8761 tcgtgtaatt ttagggatgc ttttgaccga taccggtttc cgtcattggg ccgcccgtcc
     8821 ggcggtacag gccgggttcg tgtttttagt catgctcgca gcaaacctcc tggtgaaatt
     8881 gctggccttt gggggaatcg aatttgaaag ccggataccc tggaccctga acattgcctt
     8941 tctattgctt ttttcaattt acaacgtggt gctgggcctg ttgtattcca cggggtacag
     9001 gtactggacc tattcgatct cctgttttat aggactggct ttcctcagcg cctggtcggc
     9061 aaaatggctg agtcaccttt ccatggatga agccggcagt tttcgctggc tttatctggt
     9121 gttttttatg gtgtactttt tgatcatgat gatcaccctg gccatccgga aaatcgtgtc
     9181 cctggccgag caggacgacc accggtttga tgaggtcaag ccaaaagaca attaataaca
     9241 ataaccaatt aatatgaaaa tcatcctttt cctgtttacc ctttacaccg cttgtaacca
     9301 gctcaaccag caacccatgc ctgtcacgga aagcgtaacc gccgccgcct cagataccat
     9361 taaaaaaatg gtaaaatcgg aggaagaatg gaaaaaggaa ctctccccaa tggaattcct
     9421 cgtcctcagg caaaaaggaa cggaacgggc cttttcaggg gatctgtggg accatcatga
     9481 gaaaggggtc tatacctgcc ggggttgcca gttgccgctg ttcagttcgg ataccaagtt
     9541 cgaatccgga acgggctggc caagttttta ccaaccgatc tccagggaaa gcctggccga
     9601 ttatgcagac aatacccacg gcatgagccg caccgaagtg gtttgtgcgc ggtgcggagg
     9661 ccacctgggc catgtattcg acgacggccc caggcctaca gggatgaggt attgcatcaa
     9721 cagcgtatcc cttgactttc agaaaagata accgggtgcc gctgaaagca atagggtttc
     9781 tgggatactt ctttctggcc tggtgcacca atgcccaaag cagtttttcc gggacctttg
     9841 aaggcaaaat aaatggagac ccggcgcgca tggaactcgt gcaacaaggg gacggggtca
     9901 ccggtaaata ccatgaaacc ggtaacacct ttgacatcag ggccaaggca gagggaaagg
     9961 attgccgtgg aagcctgctg atttccggaa ccgagatcgc cctggccacc ttcaatttca
    10021 ggatctcgga gaccggcttg agattaacca tcctattacc ggaccaatcc cgggtggaag
    10081 cggaatttgt caggttagct gaaccttcta aacagccggc cgggcctttt aatcatgaaa
    10141 aaaccctgac cgctgcacag gatggtttgc cgagggaccc cgcggtggtt ggccggtgga
    10201 tgaaagaaga tatcctgaat tcaggaacag ggaccagcgc tgccagcctg gtgacggtct
    10261 attatttatc cctttatgag gatggccgtt tcatacagga aaaggcaagg agcgccgggg
    10321 ggaccgattg gagcagcatt gcaaaccgga ctaccgacct cacggggcat tggtacaccc
    10381 aaaatcaggt catgtatgtc cggcctgccg gtgaaaaaga atacctgagg cttcacccat
    10441 acctttttca taacggcgct ttggtgttca aaacggaaga tgggaaatac ctgatctgga
    10501 ataaacagaa ttgaattata cccctatccc cttacttaat cactcccaat tccctgccta
    10561 cttccgtaaa tgcctgaacg gctttttcca ggtgcaccgg ttcatgcgca gcggagatct
    10621 gcacccggat cctggctttt cccttgggca cgacggggaa aaaaaagccg atgacataga
    10681 ttcccttcaa taacagtttc gctgcaaatt cctgggccag ggccgcgtca tagagcatga
    10741 tggggacgat tgggtggctg ccgggcagaa tgtcgaaacc cgcctcatga atagcctggc
    10801 gaaaccaggc agtatttttt tcaagtttat ccctcagtgc cgtggattca ctcagcaatt
    10861 ggatgacgcg cagtgaggcg ccggtgatgg aaggagcgag ggtatttgaa aacaggtagg
    10921 gcctggacct ttgcctgagt atttcgatga cttcgcggcg ggaggaggta aatcccccgc
    10981 tggcccctcc caaagccttg cccagggtac cggtgatgat gtcgatgcgt cccatgacat
    11041 cccggtattc atgcgtcccc ctgccggttt tacccataaa accggtggca tggcattcgt
    11101 cgatcatgac catggcatcg tatttctccg caagattgca gatgaggtcc agcctggcaa
    11161 tggttccatc catcgaaaag accccgtcgg tcacgatcat gcggcggcgg gcggatcccg
    11221 attccctgag tttttcttcc aggtcctgca tgtcgttgtg cagaaagcgt ttccggcccg
    11281 ctttacacag ccggatgccg tcgatgatgc tggcgtggtt gagttcgtcc gatatcacga
    11341 catcttcttc ctggagcaag ggttcgaata cgccgccatt ggcatcaaag gccgcggcat
    11401 aaaggatggt atcctccatg cccaggaatt ccgacagggc gccttccagt tttttatgga
    11461 tatcctgggt accgcagata aaacgcacgg aggacatgcc gtaaccatgg ctgtctatgg
    11521 tttctttggc tgcccggatc acctccggat gactggagag accaaggtaa ttgttcgcac
    11581 agaagttgag cacttccccc gcttccaggg tctggatcac cgcgccctga gggccggtga
    11641 tcgtcctttc ttttttataa aggttgttcg ctttgagctg atccagctca tcccttaaag
    11701 aggtcaatac cgattgattc atgatcagga tttgggtttg gtggatttga cgcccgcgtt
    11761 ttttacattt ttttcaagcc actggtccat ttcgtagagc atgtggtaga tgctttcttt
    11821 ggcggcgtaa ccatgacttt cgtggggcaa catgaccagg cgggcggtgc ccccgtttcc
    11881 ttttattgcg tgaaacaaac gttcactttg catggggaag gtgccggtat tgttgtccgc
    11941 ctcgccgtga atgagcaata tcggttcttt gatcttatcc gcatgggaaa agggagacat
    12001 cgcatgatag acctcggggg cttcccagaa acttctttgt tcgttttgga atccgaatgg
    12061 ggtgagcgtg cggttataag ctcctgaacg ggcaattccg gctgcaaaca aatcgctgtg
    12121 cgccaataaa ttggccgtca taaaagcccc gtaggaatgc cctcccacac cgatccggtt
    12181 cttgtctcct atccccatgg aacttatctt ttccactgcg gcttcggcat ttttcaccaa
    12241 ttggtcgata taggtatcgt ttggttcctc gtcgcccgaa cccacaatag gcatctcggt
    12301 ctgatccatc acggcatatc ccctgagcgc ccagaaaaca ggagaaccat aggtgatccg
    12361 ggtgaaactg tatttagagc cccgcacctg ggccgcatcg ctggccgatt tatattcccg
    12421 cggataagcc cacatcaaaa cgggtaaagg cccggatttg gttttatcat atcccttcgg
    12481 agtgtacaac actgcggtga ggtttagccc atccgccctt tggtactcta ttttttcttt
    12541 tttcaggttt gccagctggg gctggggatt cggaaaggag gtgagtgcag tgggattaag
    12601 tcccttgtaa ttcctcagat aataattcgg gttttccgtt tgggattccc tggaggtgat
    12661 aaaactgccc tcctgaagat cgatcacatg aaccgggtat tcataataag gggccttgca
    12721 tctccagagg atctcctgtt ttttggattt caggtcaaag gaagaaagga aaggcatatc
    12781 gccgtccgga gaggcccctt tgccgatcgt atagatcagg ttttttgcgg ggttggtcat
    12841 cacgacctgc cggtggtatt gattttctgc caggaccggt tcgccgggat cattataagc
    12901 atcctgcgag gaataagcaa aaagggtatc gggcgcgagg tcgggaagaa agggattaaa
    12961 tttctttatc aggatttgcc tggtggcaaa caatccttcg cgggcgaagg cgaggtattt
    13021 atcggaccag tacacgtcct gcagcctcat gccggattgg tatagagtca gggggctgcc
    13081 gctgaaaggg gcttcaagtt gctggagggc atcccggaat tcaacctttt ttttgggatc
    13141 cccttcgtcc agggcctgta cccagcaaat ggtggcggga aggtcatccc tccactggaa
    13201 attgcggggg agcctggacg cggcgtcaaa tcccctgggc ctgacttcat cggggggatt
    13261 gtccgcgagg atccgaactg ttttgcccga gaggtccagg atctcaatct gggtggcaaa
    13321 gcggctgtat gggacgaggt aggaaaatgg ttttttaata cggtgaagca ggatgtattt
    13381 tttatccggg gacaagtcaa aacggccggc gatcagatcc ctgcttaccg gcagggtccc
    13441 ttccgcactg atccggacca ggtccacgga acaataatat tcgaaaagcg cttcatcaaa
    13501 agggtttttg agcaagtcct ggaatgtggc ggcaggagca gccacgcctt cggtctcctg
    13561 gattacgggc ccaggcggca ctggattttc ccgggggggc tgcttgcctt cccgtacggc
    13621 cttggcgagg atctctgccc catggaccca ctggtacgga tttccggtgg atgaattaat
    13681 aaaaacattg ctccaccgct ttgttttcct cgttttgaga tccgccaccc acaattcatt
    13741 gccctgggca gattgatgga tgaatgccag tttttgttcg tcaggagaaa gacgtacaaa
    13801 ggaaaacttt gggttctccg gcaaaccctc caaatcgtat tcgcggccgg accggctttc
    13861 tttcagtttt aaggagacta tgaatggtat cctgctttga gaaaaggtcg ccgggttgat
    13921 gcgaagacct cctatacgga gttccggttg cgccaggtca gcaatgtcca tcatggcctg
    13981 tctttgctga atgatcatcc agccgccatt cctggtgaat aaaaccgaag gggagggtgg
    14041 ggcgttgacc aggtccacca ggtccgcaga aggggtttga tacgggttgg tttgatcctg
    14101 actttgaagc tttaaacaaa gcaacaagag gctgaaaaaa aacagtcttt tcatgtcttg
    14161 caatttattc attttttacc tcccgcgaga gggagggagg ctcaggtttc cggtcttacg
    14221 ggcgcaccaa gatgggccag catatcttgg gtcatcctgg aaaggtcaaa agtttcccgc
    14281 cagccccagt ctttccgggc aacggaatca tcgatgctct ccgtccagct ttccgcgatt
    14341 ttttgccgga agtcttcttc ataacggatg gtaaaatccg gaaggtgttt tttgatttcc
    14401 cggtaaaggg ttccggggct tacactcagg ccggaaaggt tatagctggt acggatactg
    14461 atttgcccgg caggggcttc catcagctca atggtggccc ggatggcgtc atccatgtac
    14521 aacatcggca gaaaagtgtc ctcactgagg taggaggtat aggcctcctg ccggatggcc
    14581 gcgtggaaaa tttcaacggc ataatccgtg gtacctcctc cgggagcgct ttgccaaccg
    14641 atgatccccg ggtaccggag gctccgcaca tccacctcgt acctggaatg ataatattgg
    14701 caccacagtt caccggccag tttgctcatc ccatagacgg tcgaaggctc ggcggccgtg
    14761 tgctggggcg tattggactt tggggtggtg gatccgaaga tggcgatggt gctgggaaaa
    14821 aaaaccttgt ccatctggta ttgatgggcc agctcgagga tgctgagttg cccattcagg
    14881 ttcacgttcc aggtcttttt gggattccat tccccgttgg cgctgaggat cgcagccagg
    14941 tgatagacct gggtgatgtg gtggtcttca atgatttcac tcatgcgctg gacattgagg
    15001 atatccagca tttcaaaagg cccgtgatct tctttcggtt tgtagatatc cgtgcacagg
    15061 accttgtcac gtccgtaacg gtcgcgcaag gccgcagcca gcgccgtccc gatctgacca
    15121 ttagcgcccg tgatcagaat tttcattggt cgcatcgggt aaagataatc aggaaaaccc
    15181 tgatttggat tccgggaaaa tactcaggga atataggccg gaaggaagtc gacctccctc
    15241 catcgtccat cctccgattt ttgaaaaacc ctgcattcca agccgttgac gatgcagaga
    15301 aaatcagcct tcacggccag gttatacctc acgacctgcg agacgacctg ttcatcaagc
    15361 atgacctccg gcgatttgca ttcgatcagg aggaaaggcg ccagggcgga agagtaaacc
    15421 accagatcaa aacgcttaat ggtccggtcc atccggatct gtttctccac ggcaatgcgg
    15481 gaggccgggt attctaattt gcggatcagg tagactacaa aaagctgccg caccagttct
    15541 tccggcgtga gtgccagcca ttttttccgg atggggtcca gcacggttct ctgtccattt
    15601 ttcccggaga atttaagagg ggtggtatca ctcaggaggt ttaggatcat gcttatttcg
    15661 aaaggattat gcctccgctg actttttttc ccgctctgta gacttcttcg atctgtttgg
    15721 tgccgtcctc gccatagaaa cggtaaaagc cgtcctgcac atcgtcctta aattccatct
    15781 cccgctgcat ccctcccctg aggtaatatt ttatggcccg gccgtttttc ttgcctgctt
    15841 tgtattcaat cctttcgtcg aggtgcccaa attggtaacg aatgaaggtt ccttccagca
    15901 ggtcatccat ataagaggcc tgctcttcga tctggttgtt ttggccgaag cggaggtata
    15961 agccgttttt acggtcttcc aaataattgg ttatggtttc cacttgcccg gaaggatgat
    16021 aagtgaccca agacccgttt ctttttccct gaaaaatgac tccctccacc aaaaggtttc
    16081 caaggctgtc gcgtttaaag accttttttt ggtcggaacc ttcaagagac acggtttcat
    16141 aggcgggggg cggacccttg gctttccggc atgccgtgct gaagatcaaa aattcagtaa
    16201 acaaaatgga ccatagcatc gccttgcagt tggccagaga aaactgcagg gcagggggca
    16261 tgcacgttat tttggtcatg gtctggattt gatctgggga gtatctgcct ggtggctaat
    16321 gacaaaatca atcagttctt ctaccggcat gggaattcgc tccgcagcat cccggatatc
    16381 ttccctggag acctgagcgg cgaaggtctt gtctttaagc ctttttaaga ctcctttggt
    16441 ttccatgcct tgataaccac cgggccgcat cagggaatat gcgtgcacaa aaccggacaa
    16501 ctcgtcaaag gcatataaca ttttatccag cctggtcttc ggttccacgc cgaaatgggc
    16561 cgggccatgt gccgcgatgg catttatgac ttccgggtcg gtctgccttt gttcgagttc
    16621 ttccactatt ttctggcaat gggtctccgg ccatttttcc caatccgcat catgcagcca
    16681 gccggtgacc tgccatttcc attgatggtg ttcatcgagc ccctccttct cttttgccca
    16741 gcaccggagc agccagccta cctggaacat gtggtttctg agcttttcat tggggaccca
    16801 attttccagt aagtcacggg cttctttttc agtaggccaa ttgccggcat ttgccggatc
    16861 accaaacgcc tgcctgttca gcatccttgt cattggacaa atgtaaatca aaacctctaa
    16921 cctccaacct cggacctcta acctctaacc tcggacctaa aaaaagaaag gccgtgcccc
    16981 ccgacacggc cctctgtaat ttattgatac taggttcaat aaatattagt tgaatatgta
    17041 gcgggcaccc agctggatct gccatacgtc ggtcaggcgg cttccataac gggtaccggt
    17101 ttcagggaat ttattgctga tttcggtaaa acgatatacc ggttgattcg tagcggcatt
    17161 tctgctcctg aactgcaggg gattggtcgt cgtggtgatg gttcctactc cccattggtc
    17221 attgatcatg tttccggcat tcaccaggtc cgccctgaac tgcagggtat ttttctttcc
    17281 ggcaatattg acgttgatat cctgcgtaag gcttacgtca aaggtggtga gccagggaag
    17341 caggaaacca tttctttccg cataggaccc ccggttgtca ttgagataac ccacgctgct
    17401 caggaattta tcataggctg ctttctgagc atccacggta atggtcctgg catttgcgcc
    17461 ggaacccacg gtgtactgtt caaactggat atcgttggga ttcttatgaa taaataaaag
    17521 gtcgttggta gtcagtccgt cgccattgac atccccgttg acggtatagg tcacccttcc
    17581 ctggtttccg gtctggaagc cgaggttgat acccgtacct ccccagccgt aattgaattt
    17641 ataataaagg gccgcgatga tccggtgcct ctggtcgttg tccgaaaagg ccagttcctg
    17701 gcggttgttg ccaaggcggc tgggattgtc cctccaggag gaaaaggcga tagatcctgc
    17761 cgtcatcagg tctttggctt cggcaaaatt gtatgcgccg ttgaaaaaga aattccgggc
    17821 aaattgtttc tggatctgtg ccgtgagcga ataagtatag cctttgctcg aattggtcag
    17881 gagtatggca tcggtgatct tgtcgttgat gcggagtgca ttggcctgag tagtcccgga
    17941 caatcccaaa ccaggataag taggccttac gtccggcagg ctataagctc cagttgccgg
    18001 ttccaggttg gcattgatat aatacacctg gttcaggttc ttgctgaaga tcccttccag
    18061 ggtccccacc atacccaggg gcagtttcat atccacaccg atattggtcc tgagcaactg
    18121 ggggaatttg aaattggggt ccgtaacggc gatattatag gttgggggag aggtggcatt
    18181 atccggaata tgggcggtga catcaggaga aaagggatag gctttggtgc tgttgacaga
    18241 cagcgatccc gtaagcaccc cattgtttcc gacctggttg gaaatccata cgaaggcagg
    18301 ccttccggag aagatcccgc ttccgccccg gacctggaaa gtccggtctc cgttgacatc
    18361 ccagttgaat cctaccctgg gagaaaataa gactttggcg tcgggaagtt tggaggtgct
    18421 caatttcaaa ggacttcctt cggcatcttt gaaagtaaag gtctcgactt ccgcgttgtt
    18481 ccttgccgta ttgccgaaga aaggcatgtc cgccctcagg ccaaccgtca gtttcaaacg
    18541 gtctttgaac agggcaatat catcctggag ataaactccc ggctggtagg cacgggtcgt
    18601 cgcagtggga agggccttcc cgggcagggc tgaataagtc agcacatacc tggccagggt
    18661 aatgtctgca tttccgttgg ccgcagcata aaaatcatcc agcgaattaa agatgtattg
    18721 gccgtagtag gttggggtaa aggtgttgtc aaacttaaag gattcgaagt ttaccccggc
    18781 agtgatggta tgctggcccc ggtaaaaggt gaggtcatcc ttaaattgcc aggtatccgt
    18841 gttcaggata ttgttggggg tgaaaggttc atatccgaaa gtggtatagg tggttccgtc
    18901 cttcaggata tccaccaggg ggaatacccc tcctccgctg ctccggtaat cgcggttggc
    18961 ggtgaaaccc agctgcatat tgttggcaaa tttggtgccg atgatgctgt tgagttccac
    19021 gatgccggaa tagaggtcgt tgttgatgat gtaattggaa ttctggaagt tcagtgcgaa
    19081 aaggttattg ctgcggttgc cgctgaatac gccgctgttg gaagcggtca cgtccctcag
    19141 ggatttgaga taattaaagc ggacgctcag gcggttgttg tcgttgatgt tataatcgat
    19201 tttagctagt cccttgtggc tttccgtccg gagggggaag ttttcataaa gccctggatc
    19261 gtagctgaat ttggatttca ggaaagtgga caactggtcc aggtcagaag ccagcacccg
    19321 ggtgacgtta ttgcccgtgg tgcctctgtt ggcaatgaag gtggaagaag gatccgttct
    19381 tttttcctgt tcaaaattga taaaataaaa caatttgttc tttataatcg ctccgcccag
    19441 ccgacctccg aattgctgga cgtcaaaatc cgaacgcaca atagacgttc ccgcagcttt
    19501 cttaccggtc aggctttcat tcctgcgatt gtaaaagacc gatccctggg ttttgttggt
    19561 gccggagcgg gtcacggcat ttacgccggc gccggtgaat ccaccctgcc ggacgtcata
    19621 gggggcaagg ctgacctgga tctcttcgat ggcatccagg gaaatggggg tggaactggt
    19681 ctgtcctccc ggtacactgg ccaggccaaa actgttatta aaaatggtac cgtcgatcgt
    19741 caggttatta aaacggctgt cctggccggc gaaggaggtc ccggcgccgg tggtggaagc
    19801 cacggaggaa gatcgggatt ggggaacata cctgagaaaa tcgtttaagc cccggctgat
    19861 cgtgggcatg gcttcaagaa gctctttttt aatattggtt cccgctccgg tacgttctga
    19921 actcagtacg gagcctttgt cgctggtcac gatgatctcc tgaattaccg cggattcgtc
    19981 ggccaggctg acattgaact gaagggtctg gccgagggaa agggtgatgt ttttttcctg
    20041 ataatcctta taacccacat aactgaccgt gatggtatag ggaccgccga tacgtacatt
    20101 gggtagggca aatcgtccgt cctcccgtgt agaagtcccg tacctggagc cggaaggttc
    20161 gtgcagggcc aacacggtgg caccgatcag cgcttcgccc tcgctgtcgg taatcaatcc
    20221 cgcaatgttt gcggaggtaa caccctgtcc gaatgaccat ccggccagaa tggagaaaaa
    20281 taaagtaata aatactttct ttgggataaa gtgaaagtcg ctcatatttc tcaggttata
    20341 tttgatttcg cgggcaaaag tacgatgggg atattaagaa aatattaatt taagaaaaat
    20401 tgtatatatt tttttcctat ctatgttctt ttgaaaatcc caaaattttt agtatgctcc
    20461 agatgaatta tttgcaattt tgaccgggga ttgtgccaaa atcaattctt atccatgact
    20521 cctttgcaag aagaaattaa acggctcctg gaagaacatc aagccgtaaa aaaatccgaa
    20581 tcccgcctgg gagaattgag ctccgaaatc aaccaggcca ggcaggacct tgcccggctg
    20641 gaaaaaatga tcgaaaagga ataccaggat atggaaaagc tggaaaagat cggtatgaaa
    20701 gccctttttt ataaagtgct cgggaaccag caggaacagg tggaaaaaga aaagcaggaa
    20761 tacctccagg tctccctgaa atacgatcag gtaaggaaaa accttgagct cctggaattt
    20821 gaaaacaata ttctccgcaa aaagatcagc gccggccctg agatcgaaaa aaggctcaac
    20881 gaactcagcc tcctccagga aaaagaactg atgaatgcgg agagcgccgg cacttccgaa
    20941 ctccgtaaac taaacggcca gatcgaaacc ctgcagaggc taaagatcga tgtggacgag
    21001 gccgccaaag ctggtgaaaa agcattggaa tgcatcgatg gggtggtggc cggcctccag
    21061 caagcccaca actggggaca atgggacatg tcgggccgga acccgatgag caatcacctt
    21121 aaatacactg cgatcgacaa ggccaaggac tgggcctacg aggcaaacca ccagcttaaa
    21181 cgctttgaga aagagctgat cgacgtgtac cgcaacattc cgcaggattt tcagcttcag
    21241 ctcgataatt ttaaccggtt tatagatgtc ttttttgaca acctgatcac ggactggatc
    21301 atccagcaaa agatttacaa cgccctcaac aataccctgg ccacccggga caaagtggtg
    21361 agaatgttaa aaaccctgga tgcggaaact cccaggatca accgcaatat tgaagagctc
    21421 gaactggcga aaaaacagtt tgtccttaat ttttcgaaag gcctgcaata atgaaaggtt
    21481 aagggatacg gtgcgaatac gctccatcca aaaacttaaa atacctggac ttgcagactg
    21541 gttttattct gcggtacccc cggccttgac gatatactta tcgatatcgc tggcttccgg
    21601 agaaaggggg aattgggttt tcagttcgtt gaactggctg agcgcattgg cgttgtcttt
    21661 ctgatacagg taaagaagcc cggctttttt gatataataa ggggtcagca attcattttt
    21721 ccccgcggcg atcgcctgtt tgtaataact catggctttg ttgaagtctt tcttttccga
    21781 ccaggcatcg ccaagattgc cggcccgggt aatggtcgtc acttcatcct tgggactgaa
    21841 atcttccagg taatcgatcg ctgcatcata ttgtcccagg tgcaggtatg aaatgccggc
    21901 gtaatacttg gccaggttgg aggtcttcgt ccctttgtag gagtcaatga tgtccaggaa
    21961 tccggaatag ccgccgccgg gattggtcag ggccaaccgg aaggagtctt tttcaaattg
    22021 ttgctgggct accatcatct gttccaccgc cctttcctgc cggggctgga gatataaaaa
    22081 caggtaggcc aggtatcctg ccaggccgag gaccaaagcg cccccgataa tggaaatctt
    22141 ttgctgatgc tgttcaaaaa aacttttggt tttctgctgc acttcaacca gatcaagcag
    22201 tttttcgtcc gcttttttca cacttgattt aacttttgcc atgccaggtc ttgaaaaatt
    22261 tgcgcaaaga taataatatc aacccatcga accaaatccg gctttgtcta aaaaactaat
    22321 ttgttaaggc ctcaaaatca gcgggagatc agtcccggag gaagggataa tcgggctgta
    22381 cgtagttgtc ggtgtataat tcttctgcgg gcgggaaggg ggattcttcc gcaaaccgaa
    22441 cggcttcatc catttctgcc ttgagttggt cctggatggt caggatctcc tgttcggtgc
    22501 agatggattg actgagcaat ttttcctcaa gatttgaaat cgggtccatg cccatgtaat
    22561 ggtgcaattc gtccttggtc cggtaggtgg cgggatcgct caccgaatgc cctttgtacc
    22621 ggtaggtcat gatttcgagg aaatagggac cgttccctgc ccggatataa gcggcggctt
    22681 cggtgagcgc ttcgtgcact ttctccacgt tcatgccgtc cacctgtttg ctgggcatgt
    22741 tgaaggcagc tcccacttta taaatatcca ccatattgct gctccggctg accgaagtac
    22801 ccatggcata cccgttgttt tcacagatgt acaggaccgg gaggccccag gtcatggcca
    22861 tgttggcgct ttcaaataaa ataccctggc gggccgcccc gtcgccaaac atggtgacac
    22921 agatattgtc cgtgcccttg tatttttcgg caaaagcaat gccggtgcca atgccaatct
    22981 gggctcccac gatgccgtta ccgccgaaaa agcggtgttt ttctgaaaaa aaatgcatgg
    23041 aaccgccctt gcctttgaca caaccgtcaa ttttaccgaa caactccgcc atgcaggatg
    23101 aagcggtcag gcctcgggta atcgcaatgc cgtgctgacg gtatcccgtg atcaccgcat
    23161 cctcgggccg gatggccgta gcgagcccgc cggcaatcgc ctcctggccg atgtaaacat
    23221 ggcaaaaccc gcggattttc tgctgactgt acatgaggag ggtcttctct tcaaaccgac
    23281 gaacacgaag catggtctca taccatttca tgtaggtggc cttgtcataa cgtacttccg
    23341 ttccggctaa gggtttttcg attacctgca tgttttacga ttttacggca aagataaatg
    23401 gaacaagcta tatcctccgg ttgatttttt cacaaaatgc gaagcggtcc gcatttggta
    23461 aaatgtcgat ctttgaggct caaacctatc cgagatctat atttccagtt tgatattgac
    23521 ccagtttaaa aactatacgc atcaggcgtt ttcattccat ccgggattca acctggtcta
    23581 tgggaccaac ggcagcggaa aaaccaacgt attggatgcc attcattacc tggccctcgg
    23641 gaaaagccac ttcgtgcccc aggacaaatt ttccatcctg cagggggaat cttttttcag
    23701 gattgaaggg ctgtttaagc aggaggaaga aacggtgcag tgggtgttga aatttcaacc
    23761 cggtacaaaa cgattggaga aggacgggaa ggcctatggc cggatgcagg accatatcgg
    23821 ggaggtgggt ttggtcatga ttgcgccgga tgaccttgat ctgatcgacg ggcagagcca
    23881 ggccagaagg aaattcatcg acctcacctt atcccagtcg gaccacatct atctcggcca
    23941 ccttgcaacc tataagcggc tgctggacca gcggaatgcc ctcctcaaat cttccccctc
    24001 tccggacctg ggttttttgc aggccctgaa tgaaaaaatg acggaacccg cccaccggat
    24061 ctattccata agaaaaaaat ttgtccttga ttttctgccc ttgttttaca aatattacca
    24121 cgccatcagc aaggcaaaag aaaatgtgga aatacgtttc cagtctgacc tggaccaggc
    24181 ggacctgctg accctcttca accagggatg gacacaggac ctgtatgccc agtatacggt
    24241 ccggggatgc cataaagatg acctggtgtt tttaaaagac ggcgaaccct tgaaatacct
    24301 gggctcccag gggcaaaaaa agaccttcct ggtcagcctg tttcttgcgc aatccgatta
    24361 tctttccaaa accatggaca gcccgccgat catcctgctc gacgacatct ttgacaaact
    24421 ggacgacgag cggatccgct acctcctgca caccatcggg aacatgtcca cccggcaggt
    24481 gatcattacc gacacgggaa aagaaaggtt aagtgcggtg ctgaaggacc tgggcgccca
    24541 ggttcatttt atccacatcc gggatggcct cgtcaatacc cccctccatg atgaaacgac
    24601 ataacgacct gccgctttct tccgtgctca aacaatgggc cggttccgaa aaactgaaac
    24661 ccatgctgat ccagaaaaac ctggaagccc actgggaaga atggtttgga aaacttattg
    24721 cgaaaaacac cgaaaaacta tcgatccgga accggaaatt atacatccac attaaatcgg
    24781 ggccggttaa atacgaactg aacctgggca gacaaaatgt gatgaacctg gtcaatcaaa
    24841 aacttggaga aaattacctc gaggaagtcc tggtcctctg acctcgattt ccgccgttga
    24901 attcgggaag ttcacgagta gcctaaggag ttgagactta ggaacatatt aaaattttt
//
LOCUS       DAOQXT010000070        24896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2332_length_24896_cov_5.962683, whole genome shotgun sequence.
ACCESSION   DAOQXT010000070 DAOQXT010000000
VERSION     DAOQXT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24896
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2332_length_24896_cov_5.962683"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1025
                     /locus_tag="PKM27_12540"
     CDS             177..1025
                     /locus_tag="PKM27_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNR08140.1"
                     /translation="MSVFLRIIKIVLIAALVVLNENKDQLLKSLGITSYLVGAIIGFL
                     LFFLIAELVKDLLLYLYRNRKKMKPRQTDNVTYGLENIYILFLAGALFYSVLYMFKLK
                     PREFFTGFTLISAATAIVMKDYVSNIISGMILAFSDKLKIGDYVRIGRHTGEILNFSL
                     SMITMRNDDDELVYLPINLVFTTDFINYSPGQEDFITVPFEINHKMRVPFEELQKRIA
                     ENLAPFHPNIQPGSGQLRVAANNQETTSFKYQYVLKTPDILLARRIKETILKTVFDST
                     SLPKAV"
     gene            complement(1229..1687)
                     /locus_tag="PKM27_12545"
     CDS             complement(1229..1687)
                     /locus_tag="PKM27_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08141.1"
                     /translation="MKIRNLVATVVVALFVQVGFAINSVKNTVAGNGVALPIELATGH
                     GMTRSEAKAIQKLEKKQVRAQKRVALVQKYLAKNMAKKAIDFKDPVNKWLWFGVFGWG
                     AGLVLWIIAAAVVTGGGFLGGGFLGVLASLAWLFGTVAFVIWLVKKFGDA"
     gene            complement(1768..2145)
                     /locus_tag="PKM27_12550"
     CDS             complement(1768..2145)
                     /locus_tag="PKM27_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNR08142.1"
                     /translation="MADRRIMMLIFISGALAVLLGAFGAHALSPYLDEAGTQTFNTAN
                     RYHFYHTFLAALLGVWKPGDRLARYGQVLALAGIVLFSGSLYLLAFKTVPGVPSWLGP
                     ITPMGGLAFILAWSTGILKLIKN"
     gene            complement(2127..2807)
                     /gene="ung"
                     /locus_tag="PKM27_12555"
     CDS             complement(2127..2807)
                     /gene="ung"
                     /locus_tag="PKM27_12555"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNR08143.1"
                     /translation="MATDVKIDASWKAVLQEEFDKPYFQAIRDFIKSEKEAGKVIYPP
                     GPKIFNAFNHCTFDQVKVVILGQDPYHGPGQAMGLSFSVPKGIPLPASLKNIYKEIER
                     DLQVKMPRHGDLSAWASQGVFLLNAILTVEQSKAGSHQHAGWQTFTDAVIRILSEQKN
                     GLVFLLWGNFARNKKNLIDQRRHHVLEAAHPSPLAGGAFHGNHHFSTANRLLLQSNKT
                     PIQWQIEE"
     gene            complement(2843..3664)
                     /locus_tag="PKM27_12560"
     CDS             complement(2843..3664)
                     /locus_tag="PKM27_12560"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNR08144.1"
                     /translation="MKIPALRIVLLGGGNLAYHLSKALSGLPGYPLVQIWTRSPKQAR
                     ALGVRSNCAFTHRLHELDSRANLYILCVSDDSIGSLAGELTGFIPPDAAVIHTSGSKP
                     STLFKSLYNNYGVLYPLQTFSKQKKINFSGIPLFITASNRSTEALLSHLAHQLSQEVR
                     VIQDSARLKLHLAAVLVNNFTNYIYTLTDDYLQSQGLDLRSFYPLIEETLAKLKTMKP
                     AQAQTGPARRHDKTVIDQHLSLLKKLKQEELQTIYRLFSTMIWKKYPLTGPPGRD"
     gene            complement(3681..6473)
                     /gene="ccsA"
                     /locus_tag="PKM27_12565"
     CDS             complement(3681..6473)
                     /gene="ccsA"
                     /locus_tag="PKM27_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNR08145.1"
                     /translation="MPPIEYLGEHRWIGHVLHASVIIGFLASLNAIIAYSKSVRNPAS
                     PWMKYGRMAFYLQGLMVFATIFLIFFAMLNHYYEYEYVRHHISDDLPFKYIFAAFWEG
                     QEGSFLLWMFWNVILGFILIRKSGPWEAPVMALFATLQVLLFSMLLGIHIGWGEHLVK
                     LGSSPSLLVRETFTAPIFENPNYVSLLKGNGLNPLLQNYWMTIHPPTLFLGFSSTIVP
                     FLFAFAGWWKNEPKAWLKPAQEWALFAVFILGLGILMGGAWAYEALSFGGYWAWDPVE
                     NMSLVPWIMLLAGLHTNMIARSTGRAVGTTYIYYALAFILVLYSTYLTRSGVLGDTSA
                     HAFTDMGLEWQLILMIVVFTFLSAYQYLTKRKLVASNSEEEKIYSREYWMFIGAMVLL
                     FSAILITGATSLPVFNKIIKAISPEYLGSVIVDPISHHNKFQLWIGVLVGLISGSAEF
                     LRFKETRWAEYKPRFLKLMGSSLAITAGLFLLTMRLLNEPSWQHLVLQFAGIFALVSN
                     LAYLFTFLRGKLKLAGSALAHFGFGMMLLGILATGLNKQIISSNLFAQSDLLEGFSTE
                     DYMRNLALIKGAPMPIKDYEVTWMSDTLLDPFNRQFQIRFDHKDESGRVIKKINVSPT
                     LIYNRNEAQPPSSNPSIYRGLLKDLYTFVNWLPPAMMNREAAAKIEDSLRYIPHLVAV
                     GDTLFFSGGYGVLLSVDDSARVPEYKAEPGDQVLRASVQFHRLNDTGSYLLQSVIVSR
                     ENYYFSLPQTQNDLALKVRISPLSLENFYPSSLEGDDLELKENQSGKIRGYQLRFLGF
                     DKDPAPSRYASRPNDVAVATRLEISSPDGAVRLAEPLFVIRDNQVFLVPDYILDQALK
                     VSCIKIDPQKESVLIRLYESVENKIQLELAENAPRNDMIVIQSILFPGINLFWLGSFL
                     MLGGVLLSGVRRWINR"
     gene            complement(6477..6869)
                     /locus_tag="PKM27_12570"
     CDS             complement(6477..6869)
                     /locus_tag="PKM27_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNR08146.1"
                     /translation="MNKLHIIAALVLITGMALFFSLSRDVSTYATFAEATENQHRVKV
                     AGTLAKDKEMYYNPAENPNYFSFYLRDPKGAEKKVILLQGKPQDFDRSEQIVVTGEMK
                     GDEFVANEVLLKCPSKYKDEEIYMKKQI"
     gene            7034..7504
                     /locus_tag="PKM27_12575"
     CDS             7034..7504
                     /locus_tag="PKM27_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HNR08147.1"
                     /translation="MRWRKFDGQIIEVPILEAVEKTLVRESEAGNRLKVCIGTDSEVR
                     QDGIEYATVIVFLREKKGGFMFICNQHRPRIMSVKERMITEVSMSVEIAYHLCDLLDR
                     YGVGLEVHADINTDPHFKSNEALKEAMGYILGMGFVFKAKPDAFASSYCADKVV"
     gene            complement(7564..7647)
                     /locus_tag="PKM27_12580"
     tRNA            complement(7564..7647)
                     /locus_tag="PKM27_12580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7611..7613),aa:Leu,seq:cag)
     gene            complement(7703..7786)
                     /locus_tag="PKM27_12585"
     tRNA            complement(7703..7786)
                     /locus_tag="PKM27_12585"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7750..7752),aa:Leu,seq:caa)
     gene            complement(7815..8330)
                     /locus_tag="PKM27_12590"
     CDS             complement(7815..8330)
                     /locus_tag="PKM27_12590"
                     /inference="COORDINATES: protein motif:HMM:NF019363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster co-chaperone HscB C-terminal
                     domain-containing protein"
                     /protein_id="HNR08148.1"
                     /translation="MNYFEFYGLSPSLAPDPELLKKKFLEASRKHHPDHFVLKPELEK
                     EEAEEWSAFNNKAYRTLSNPDLLFKYILELKGLTGEGEGDQSGLSQEFLMEMMDIHDL
                     ILEDPVKADLQLEAAENSIRAEIDPLILHFDFDKASGAELESLKEYYFKRRYILRLRE
                     NLNKFAADQRP"
     gene            complement(8327..9655)
                     /gene="rseP"
                     /locus_tag="PKM27_12595"
     CDS             complement(8327..9655)
                     /gene="rseP"
                     /locus_tag="PKM27_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNR08149.1"
                     /translation="MTGIPIMIIQLILSLSFLVIIHECGHFFPAKWFKTRVEKFYLFF
                     DPWFSLFRTKKGDTEYGIGWLPLGGYVKIAGMVDESFDTEKLKEEPKPWEFRSKPAWQ
                     RLIIMLGGVTVNFIAGFLLFALVLGIWGEEYLPSRSVPNGIFTDSLGRVIGLKDGDKI
                     LKVGAVDFDIFDHRMLRREVVINGAKTITVERDGQVIDLPMEDRFIHDLSSNKNKNST
                     LIGPRVPVKIDRFSESSPAQAAGLAVGDEIVSLNGTPMFINDLTKTLRQLKDSSVTLV
                     IKRGGQELPFTFKTSAEGTIGAYTLPVLSVERKKYGFMESIPAGINKGLGFLSDQIKA
                     FGQMFKGKIKASESLGGFASITSLFPDTWSWEDFIRITAILSLILGFMNLLPIPGLDG
                     GHVVFLLFELVTGKKLSDKFIEKATLVGFILLIALMIYANGLDVFRFLKK"
     gene            9789..10475
                     /locus_tag="PKM27_12600"
     CDS             9789..10475
                     /locus_tag="PKM27_12600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNR08150.1"
                     /translation="MHYFFGNAGPGSIADLSPEEARHAFVLRLKPGEKVGLLDGKGTV
                     CLGEVIFSGPKGAQVLVREVRNQQAPKPHIHLAVAPVKQSERMEWLVEKITEIGVSEI
                     SPLICDHSERFHFRPDRLEKIAHAAVKQSGRPWQVLLHQPVRFEAFMSQDFRGAQLIG
                     NQNGRPLKGLFGEARPDEITLCIGPEGDFSEHELALADKKGFIKISFGDVRLRTETAA
                     LVIAGILTGG"
     gene            10585..11265
                     /locus_tag="PKM27_12605"
     CDS             10585..11265
                     /locus_tag="PKM27_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNR08151.1"
                     /translation="MKIRAYSVLVFLLTLLFFSSAFRFSQAPTLKLALLKYNGGGDWY
                     ANPTSLPNLARFCNQALGTNLDDDYATVEVGSADLFNHPFVHMTGHGNVVFNDAESEN
                     LRNYLLAGGFLHIDDNYGMDPFIRPAMKKVFPELSFIELPFEHEIYHQKFSFKNGLPK
                     IHKHDDRSPQGFGLFWEGRLICFYSYECDLGDGWEDQEVHKDPEETRLHALQMGANLV
                     QYAFNESK"
     gene            complement(11302..11514)
                     /locus_tag="PKM27_12610"
     CDS             complement(11302..11514)
                     /locus_tag="PKM27_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNR08152.1"
                     /translation="MFKSFIERSVFGVCTYLGQRLGIDNTKIRLHFIYLSFFTLGSPV
                     IVYFILAFWLNITRYIRKNNHIFHWE"
     gene            11562..11882
                     /locus_tag="PKM27_12615"
     CDS             11562..11882
                     /locus_tag="PKM27_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNR08153.1"
                     /translation="MNKEKEIKQEINIELSEEIAEGIYSNLAIISHSHSEFVIDFVRL
                     MPNLPKAKVKSRIVLTPQHAKRLMMALGDNIKKYENQFGEVGDDSDFPGLPPMNFNTP
                     TAQA"
     gene            11922..13400
                     /locus_tag="PKM27_12620"
     CDS             11922..13400
                     /locus_tag="PKM27_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR08154.1"
                     /translation="MDWKAVLENEVSLDPEDWEGAKARGRQMLDDLVEYLRHIGDEPV
                     WRQPPREMKDFLHHTLQDSGQDPDLLYAQFKKYILPYPKGNIHPRFWSWVQGTGSLTA
                     VYADMLASAMNSNLAIGDHAAMYVEQQVLDWCKSMLAYPKEASGIILSGGSMANITGL
                     TIARNASDERIRSEGVGISGKPLVFYASEETHSCQQKAAEVLGIGSKGLRKIPVDDQY
                     RMRIDLLEKQIEADLKKDCLPFCIVANVGTVNTGAIDPLEEIRQICHHHKIWFHVDGA
                     FGALAKLLPEYQDSLKAMEQADSLAFDLHKWMYLPYEIGCLLVKDRQKHRDAFALQPH
                     YLSSHERGLPAGPDPVGNYGLELSRGFKALKAWFCFQEYGLDKYRQIIRQNIAQCLYL
                     ADLVRNSDFLELLAPVPLNIVCFRFKVPGMDEEALNRLNKEILMRLHEEAVATPSYTI
                     LKGRYAIRVANVNHRSKKHDFDALVEGVVRIGRELMAAIKPG"
     gene            complement(13413..14522)
                     /gene="leuB"
                     /locus_tag="PKM27_12625"
     CDS             complement(13413..14522)
                     /gene="leuB"
                     /locus_tag="PKM27_12625"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNR08155.1"
                     /translation="MNKTIAVLPGDGIGPEVIAQSLKVLKVIENKFQHRFECTEAPIG
                     AAAMAQGLEPLPPATLELCQKADAVLFGAIGDPRYDKDPGTVFKPEQGLLDLRKSMGF
                     FANIRPIKSYRKLAALSPLKEEQVHSINLILFRELTAGLYFGKKQKDEEGRWAIDECY
                     YHQEEIERISHLAFRQARLRKKKLTLVDKANVLETGKLWRSTVQNLHKSDYPDVELEL
                     MLIDHAAMQLVLNPRQFDVILTENMFGDILSDEASVVVGTIGLLPSASLNENQGLFEP
                     IHGSYPQAAGRNLANPIASILSVAMMLDFFDLTAEAGLIREAVDFLINEGLGTMDMAP
                     RTIITCSAMGEFVSGLVASDKSSWPSMRKSLDKTV"
     gene            complement(14621..15793)
                     /locus_tag="PKM27_12630"
     CDS             complement(14621..15793)
                     /locus_tag="PKM27_12630"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNR08156.1"
                     /translation="MAEKIQIFDTTLRDGEQVPGCKLNTQQKLELAEALESLGVDVIE
                     AGFPISSPGDFQSVYQIAEIIKHSTVCGLSRSVEKDIITAAEALQPARSPRIHTGIGT
                     SDVHIKYKFNSTPEKIIERAVAAVRLAKKFVYDVEFYAEDAGRTENEFLAKVCTEVVK
                     AGATVLNIPDTTGYCLPDEYGDKIRYLKENVKGIDKAILSSHCHNDLGLATANSIAAI
                     RNGARQIECTINGIGERAGNTSLEEVVMIIKQRKHLGFFTNIHSKMLTEMSHMVSDRM
                     GIPVQPNKAIVGANAFAHSSGIHQDGVIKHRENYEIIDPHEVGVNESAIILTARSGRA
                     ALAYRYRNLDIELGKEQLDILYKNFLELADQKKEIVAEDLLRLYDEHIVKKHKAVV"
     gene            15963..17504
                     /locus_tag="PKM27_12635"
     CDS             15963..17504
                     /locus_tag="PKM27_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08157.1"
                     /translation="MPKQPTDFLLQLIKSMSKPEKRHFKLFVQRNPSNTDSLFLRLFS
                     HMEKQRAYDEKSILHQIPEIKKQQLPNLKAHLYKQLLVSLRLMHRGENEDIDVREKID
                     YARVLYNKGLYRQALDMLDKAKEKCGTFQLHSLRMEILDFEKLIESQYITRSIASRAE
                     QLSAESQYTAGEIARIQQYSNLSLQLYSLYLKVGYVRNSKDHFFVREFFQAHLPVNRE
                     QDLGFYEKVYLYQSYVWYYHMLLDFKMYYRYSQKWVELFDHHPLLAAMERPLFLKARH
                     NQLNALFLLQRHDKFMEALHKLMTELKKQPARNDNEKSLEFIYTYTHRINQHYLEGTF
                     QQGLPLVHEIMHHIKADTHHLDFHRVILFYYKIAALYFGSGDNSRAIDYLNLIVNESP
                     DFRGDIQCYARILRLIAYYEMGDNYLIEYQVKSIYRFLTKMDDLHAVQKEILSFIRKL
                     PRVHQADLRREFRKLLDKFIRLRADPYETRSFLYLDIISWLESKIQGVPVQQVIREKF
                     LRKYK"
     gene            complement(17519..17740)
                     /locus_tag="PKM27_12640"
     CDS             complement(17519..17740)
                     /locus_tag="PKM27_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08158.1"
                     /translation="MNKRQWILTGAFLCFLFGFLTLLLSLLGLEFSYLAWIDRLPWIF
                     PLLLKLILLFGGLILAYLASFDWKNGNRE"
     gene            complement(17828..18238)
                     /locus_tag="PKM27_12645"
     CDS             complement(17828..18238)
                     /locus_tag="PKM27_12645"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNR08159.1"
                     /translation="MESNGMNWLAVLVAGIAAWALGALWYSPVLFGKAWQKESGLTEE
                     NLKNANMPLIFGTSAVMMIIMSAGLAMFFQGDAAMNAMKGLQMGLVTGLVFVATSTAI
                     NYLYQRKSFKLWLIDAAYQVLLLALAGAIVGAWR"
     gene            18326..19258
                     /locus_tag="PKM27_12650"
     CDS             18326..19258
                     /locus_tag="PKM27_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNR08160.1"
                     /translation="MDFQYNQNGIYCLVEMPVENFRRSLGLKMVKLIECPRDAMQGLK
                     TFIPTEKKIAYINQLMRVGFDTIDFGSFVSHKAVPQMADTHEVIRRLEPEGKSKLLAI
                     VANLRGAEDACRYEGIRYVGYPFSISETFQRRNTNSTIAESLETVKEIQDLCAHHGKQ
                     LVIYISMGFGNPYGDPWNVHIAQYWVDELAELGIRIISLSDTIGVSTPDSIHYLFSNL
                     IPPYPDVEFGAHLHTMPRSWREKVEAAWNGGCKRFDGALKGMGGCPMAKDDLTGNMPT
                     ERMLSFFKETVPDLSIDWTELDKAMAMADTLFYS"
     gene            complement(19249..20106)
                     /locus_tag="PKM27_12655"
     CDS             complement(19249..20106)
                     /locus_tag="PKM27_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNR08161.1"
                     /translation="MKYHFIGGEIIPDSQALIPVFDLGLLRGYGVFDFFNVKQGVPMF
                     LNEHLARFLNSARQVDLVCPYGIKELRKHIIELIAKNKMITGSMKVVLTGGISSNGFD
                     PDGPNRLMILAGDLIFPVTEEGDIHPFRLKTIDYVRETPGVKSLNYLVPLLHWKESKA
                     AGFHDLLYVHDGWVSETSRANLFAVTYDNILITPSEKILGGVTRDHVIRLAKTYMPVE
                     IRPLSLQEVLHCKSVFLTSTTKKIRPVSQIDEHVFSSTECWDPAGKLLQELKSMEDQY
                     ILEFLRHQE"
     gene            complement(20108..21553)
                     /locus_tag="PKM27_12660"
     CDS             complement(20108..21553)
                     /locus_tag="PKM27_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNR08162.1"
                     /translation="MPKLLVANRGEIAIRIFRTARKKGWKCVAVYSEADRHSLHLAHA
                     DEAYCIGPAPAKDSYLNIPSILEAAKKSGAGFIHPGYGFLSEKSEFVSAVESAGLVFI
                     GPGVEAMQKLGSKIAAKQIAEQLGIPLVPGYHRPIRDDGEGIAMARHIGVPLMIKAAA
                     GGGGKGMRVVKDLSQIVEAISRARAESKASFADDSIFLEKYIENPRHVEVQIMADLSG
                     RCIAFPERECSLQRRHQKLMEESPCVFISSTTREALRRDAVRLAQACGYTNTGTVEFI
                     VDAEENYYFLEMNTRLQVEHTVTEMLTGLDLVDLQLDIALGRSLPFTQEEIRSVGHAM
                     QWRIYAEDPAQGFMPSTGQITKYLAPTLPQIRIDSGYGQGSEVEVYYDPMIAKLIASD
                     QDRFSCMERLKEALQQFIIEGIETSIPFGIRVLEHPAVLAGDLSTGFLDQHLNEILYP
                     PDLEDKYLAAAAAAYHYYRTQSGQLKLPNHE"
     gene            21598..23559
                     /locus_tag="PKM27_12665"
     CDS             21598..23559
                     /locus_tag="PKM27_12665"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNR08163.1"
                     /translation="MDSKKIFDPPPEWISSCNMTAYISWLKKNKKLDFSDYESLWKWS
                     VDRVEDFWSSILEYFQVQYTGSFEQVLSSRKMPGAKWFEGIKLNYAAHLTRKVELEAS
                     ALIACNESGQIRPYTWQRVLREAGAVQRAFNRHGLRPGDRVAAYLPNIPEASISLLAI
                     VASGLVWSSCSPEFGSASVIDRMSQIQPRVLIAVDHYRYSGKTFDRRAELEAIVNGIP
                     SLELVILVDPLPGGSGELRGLPVIPWTAVAGEEGEFPEYVEVPFDHPMWILYSSGTTG
                     LPKAITHSQGGMLLEHLKYIHFHNDVRKGELFYWYSTTGWMMWNYLHTSWLAGAVPVL
                     YDGHPAHPGFARLWDLARDLGIHHFGTSAPFIHSCMKEGLVLPELPLLRSISSTGSPL
                     SPEAYDWLYREIGHPLYLWSMSGGTDVCTAFVGGCPISPVYEGEIQCRALGCDLRVYD
                     EQGQELNNEVGELVIVQPMPCMPVYFWNDPDGHKYHEAYFEVFPGVWRHGDWIQLTST
                     GGLVISGRSDTTLNRLGVRIGTAEIYAAVQQLEQVEDSLIIHIDLKSGEAYMPLFVKL
                     KHGQLEDHLLSEIRATIRHHCSPRHVPDAIIQVKDIPYTLSGKKMENAVKKIFQGKEL
                     SLVASPGAMRNPDSLEEYQEIRNSLDLRS"
     gene            23635..24540
                     /locus_tag="PKM27_12670"
     CDS             23635..24540
                     /locus_tag="PKM27_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR08164.1"
                     /translation="MTRRPQILTIESLEKFERVINRDFRPYDINMMVMPGTLEDKHKN
                     CASIPALRRQFNLIYLLLGGEHDVRLGAEHTRLRDHDLVIVPENTVYASDHIRHCTGF
                     CIHFKTEFLQTLLQRPLSEEFPYFRMDAHHILGLSPAESKIIRQSFENIISEYQRFSP
                     EKEYILRNLILILLLRVREIYRTHTKTIRQNTSRPEQLSTRFKQLVEKHFRTERTVSE
                     YAARLGVTSGHLSDVVKKTTGRSPREVIQDMLFLESKVLLAATDKTLSEISGELNFRD
                     LAHFSHFIKKKTGHFPQAFRKQLSV"
     gene            24678..>24896
                     /locus_tag="PKM27_12675"
     CDS             24678..>24896
                     /locus_tag="PKM27_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08165.1"
                     /translation="MKLKIMLLAMIATLFMAARMHHAGVAKAEHKMFAKPDEVRTFPL
                     GKLELVKLGGAMIGRATFRSRRHWTPAAP"
BASE COUNT         6279 a         6207 c         6319 g         6091 t
ORIGIN      
        1 gggggttaaa agtcaaattt gtaaatgggg tccatgagga acaaaaataa aactattcct
       61 atgattcaaa ggaggaaggg agtcccgcag ccgcgggagg gaggaaggga gtaccggact
      121 gaacctcaac gttaagctca atcatcctta cctttgaccc atccaaacat ccggttatga
      181 gtgtttttct gcgaatcatt aaaatcgttt taattgcggc tcttgtcgtc ctcaacgaaa
      241 ataaagacca gttgttgaaa tcgcttggga tcacttcata cctggtcggg gccattatcg
      301 ggtttttgct gtttttcctg atcgcggagc tggtcaagga cttattgtta tacctgtacc
      361 ggaaccggaa aaaaatgaaa ccccgccaaa cggacaatgt gacctacgga ttggaaaaca
      421 tctatatcct tttccttgcc ggagccttgt tctattcggt attgtacatg ttcaaactca
      481 aaccgaggga gtttttcacg ggatttaccc tgatttcggc cgcgacagcc atcgtgatga
      541 aggattatgt atccaatatc atcagcggga tgatcctggc attttccgat aaacttaaga
      601 tcggagacta tgtccggatc ggcaggcata ccggcgaaat cctgaatttt tccttgtcca
      661 tgatcaccat gcggaatgac gacgatgaac tggtgtacct gccgatcaat ctcgtattca
      721 ccacggactt tatcaattat tcacccggac aggaggactt tatcaccgtc ccctttgaga
      781 ttaaccacaa aatgcgggtc ccttttgagg aactgcaaaa gcggatcgca gagaacctgg
      841 ccccatttca tcccaacatt caaccgggaa gcgggcaact gcgggtggcg gccaacaacc
      901 aggaaaccac cagcttcaaa tatcaatatg tgctcaaaac cccggacatc ctcctggccc
      961 gaaggataaa agaaaccata ttgaaaacgg tatttgattc cacctcttta cccaaggcgg
     1021 tataaaaaca aagcccctcc gaagaggggc tttcagggtt aaacatcttg ccttttgatg
     1081 attcccaatt ttcgtcttga ggtttttata ttctattctg ttacgaaatt aaatacttta
     1141 ttcaattgtt caaattttat accattattt ataaaaaaaa gccgtctgtc ggagacaaac
     1201 ggctttcaac atttatttag agtaagtctt aggcgtcacc aaattttttg accagccaaa
     1261 tcacgaaagc cacggttccg aaaagccagg ccaagcttgc caataccccg aggaatcctc
     1321 ctcccaaaaa tccacctccg gtgactacgg ccgctgcaat aatccaaagc accaaacctg
     1381 ctccccagcc aaacactcca aaccacagcc atttgtttac cgggtcttta aaatcaatcg
     1441 ccttcttggc catgtttttt gccaggtatt tctgtaccag ggcaaccctc ttctgggccc
     1501 ttacctgttt cttctccaat ttttgaattg cctttgcttc tgaccgggtc atcccatgtc
     1561 cggttgccag ttcaataggc agggcaacac cattgccggc cacggtattt tttaccgaat
     1621 tgatggcaaa tccaacctga acaaacaagg ccactacaac agtcgctaca agattcctga
     1681 ttttcatatt aacttttatt taggttttcc aagcacgaag ataggcaaga atcgcggata
     1741 ttatggctaa tgccttttgt taaattttta attcttaatc aattttaaga ttccggttga
     1801 ccaggccagg ataaaagcca ggccacccat gggcgtgatg ggtccaagcc aggacggaac
     1861 ccccggcacc gttttaaagg ccaggagata aagggaaccg gaaaaaagga cgatacccgc
     1921 cagggccaga acctggccat accgggcaag ccggtctccc ggtttccaga cccccagcag
     1981 ggcagccagg aaagtgtggt aaaaatgata gcggtttgcc gtattaaaag tttgggtgcc
     2041 ggcttcatca aggtaggggc tcagcgcgtg cgcgccaaag gcgcccagca ggacagccag
     2101 ggccccggaa ataaaaatga gcatcattat tcttcgatct gccattggat tggtgtttta
     2161 ttggattgca ggagcagccg gtttgccgtg gaaaaatggt gattcccgtg aaaggcaccc
     2221 ccagccagag gggaagggtg tgcggcttcc aggacatgat ggcgccgctg gtctatcagg
     2281 ttttttttat tcctggcaaa attgccccag agcaggaaga ccaggccatt tttttgttcc
     2341 gataaaatac ggatgaccgc atcggtaaag gtttgccaac ccgcatgctg atgagatccg
     2401 gccttcgact gttccacggt aagaatagcg ttgaggagaa aaaccccctg ggaggcccat
     2461 gcgctcagat caccgtgccg gggcatcttg acctgaaggt ccctctcgat ttctttgtaa
     2521 atgttcttca gggatgccgg aaggggaatg cctttgggga ccgaaaaact caagcccatg
     2581 gcctgccccg gaccatgata cggatcctgg cccaggatca ccactttgac ctgatcgaag
     2641 gtgcagtggt taaaggcatt aaagatcttg ggacccggcg ggtaaatcac cttcccagcc
     2701 tctttttcgc ttttgataaa gtcccggatg gcctgaaaat agggcttatc aaactcttcc
     2761 tgcaagactg ccttccagga tgcgtcgatc tttacatcgg ttgccatctg tttttcggtc
     2821 gtttaaacag ttcaaaaata attcaatcac gaccgggagg gccggtgagc gggtattttt
     2881 tccagatcat ggtggaaaac aaacgatata tcgtttgcaa ttcctcctgt tttaattttt
     2941 tcaacaggga caggtgttgg tcgatcacgg ttttgtcatg ccggcgggcc gggccggttt
     3001 gcgcctgtgc cggcttcatg gttttcaatt ttgccagggt ttcttctatc agagggtaaa
     3061 aggaccggag atccagcccc tggctttgca ggtaatcatc ggtgagggta tagatataat
     3121 tcgtaaaatt attgaccagg acagcggcga ggtgcaattt caaccttgcg ctgtcctgga
     3181 tgacccggac ttcctggctg agttgatggg ccaggtggga caggagggct tcggtgcttc
     3241 tattggaagc ggtgataaaa agaggaatcc cgctgaaatt tattttcttt tgtttgctga
     3301 acgtctggag cggatataaa accccataat tgttgtacag ggatttgaac aaggtggatg
     3361 gtttgctccc ggaggtgtgg atgaccgccg catcgggcgg aataaaacct gtcaattctc
     3421 ccgccaggga tccaatcgaa tcatcggaga cgcaaaggat atataagttt gccctgctgt
     3481 ccaattcgtg gagcctgtgg gtgaatgcac aattggatct tactcccaaa gccctggcct
     3541 gcttcgggga ccgggtccat atctggacca gcggatagcc cggcaacccc gacaaggcct
     3601 tgctgagatg ataggcaaga tttcccccgc ccagtagaac aattcggagt gccggtattt
     3661 tcaaattaaa ggcattgcat ttaccggttg atccatctcc ggacaccgct cagtaaaaca
     3721 ccacccagca tgagaaagga acccagccaa aacaggttga ttccaggaaa taaaatggac
     3781 tgtatgacga tcatgtcatt ccggggcgca ttttccgcca gttccaactg gatcttgttt
     3841 tcaacacttt catacaaccg gatgagcacg ctctctttct gcggatcaat ttttatacag
     3901 gacactttca gggcctgatc cagaatataa tccggtacga ggaagacctg gttgtcgcgg
     3961 atcacaaaca aaggttcggc caaacgcaca gcaccgtcgg gactgcttat ttcgagccgg
     4021 gtcgctaccg ccacatcatt cggcctggac gcgtacctgg agggtgcagg atctttatca
     4081 aaacccaaaa aacgaagctg gtaaccacgg atctttcccg attggttttc tttcagttca
     4141 aggtcatctc cctccaggct ggacggataa aagttctcca ggctgagcgg actgatccgg
     4201 actttgaggg caaggtcatt ttgggtttgc ggaagactga aataataatt ttccctggaa
     4261 acaatgaccg attgcaggag ataagagccg gtatcgttaa gccggtgaaa ttgtacgctg
     4321 gcccggagta cctggtcacc gggttcggct ttatactccg gaacccgggc gctgtcgtca
     4381 acggacagca aaactccata tccgcctgaa aaaaagaggg tgtcgccgac cgccacaagg
     4441 tgtggaatgt accggaggct gtcttcaatc tttgctgccg cttcccggtt catcatggcg
     4501 ggaggaagcc aattgacgaa ggtgtacaga tccttcagca atcccctgta tatggatgga
     4561 ttggaggacg gaggctgagc ttcattgcga ttgtagatca gggtggggga aacattgatt
     4621 tttttgatca ccctgcccga ttcgtccttg tggtcaaaac ggatctggaa ctgccggtta
     4681 aaaggatcca gcaaggtatc ggacatccag gtgacctcat aatccttgat gggcatgggg
     4741 gctcccttga tcagggccag gtttcgcatg taatcctccg tgctgaaccc ttcgagcagg
     4801 tcggattggg cgaataaatt gctggagatg atttgtttgt tcagaccggt ggccaggata
     4861 cccaggagca tcattccaaa tccgaaatgg gccagcgctg aacctgccag tttaagtttg
     4921 cccctcagga aggtaaaaag ataagcgagg ttgctcacca gggcaaaaat accggcaaac
     4981 tgaaggacca ggtgttgcca ggaaggctcg ttcaacaaac gcatagtgag caggaacagt
     5041 cctgccgtga tcgccaggct gctccccatc aattttaaaa acctgggttt atattcggcc
     5101 cagcgggttt ccttaaaacg gagaaactcg gcggaaccgc tgatcagccc taccaggacg
     5161 ccgatccaaa gttggaattt attatggtgg ctgatcggat ccacgatcac actgcccaaa
     5221 tattccggcg aaatggcctt aatgatttta ttgaacacgg gcaaggaggt agccccggta
     5281 atgaggatgg cagaaaaaag caggaccatg gccccgataa acatccagta ttcccggctg
     5341 tatatttttt cttcttctga gttactggcc accagctttc gtttggtcag gtactggtat
     5401 gcacttaaaa aagtaaatac cacgatcatc aggatcagct gccactccag ccccatatcc
     5461 gtgaaggcat gcgcgctggt atcccccagc accccgctcc gggtaagata ggtcgaatag
     5521 agcaccagga tgaaagcaag ggcgtaatag atataggtgg taccgaccgc cctcccggta
     5581 ctccgggcga tcatattggt atgcagcccg gccaggagca tgatccaggg taccagggac
     5641 atgttttcaa ccgggtccca ggcccagtat cccccaaaac tcaaagcctc ataagcccag
     5701 gcaccgccca tcagaatgcc caaacccagg ataaatactg caaacagggc ccattcctgc
     5761 gccggcttaa gccaggcttt cggttcgttt ttccaccagc cggcgaaggc gaaaagaaaa
     5821 ggaacgatcg tgcttgaaaa gccaaggaac agggtggggg gatggatggt catccagtaa
     5881 ttctgaagca ggggattcag gccgttgcct ttgagcaggc tgacgtaatt gggattttca
     5941 aaaatagggg cggtaaaggt ttccctcacc agcagacttg ggctggagcc cagttttacc
     6001 agatgttccc cccatccgat gtggatgcca agcagcatgg aaaacagcag gacctggagc
     6061 gtagcgaaga gggccatcac cggggcttcc cacggcccgc ttttgcggat caggataaat
     6121 ccaaggatga cattccagaa catccagagc aggaaactgc cttcctggcc ttcccagaag
     6181 gccgcaaaaa tatatttaaa agggaggtcg tcggagatgt gatgccgcac atactcatat
     6241 tcataataat gattgagcat ggcaaagaag atcagaaaga tggtggcgaa gaccatcagg
     6301 ccttgcaggt aaaaagccat ccgcccgtat ttcatccagg gactggccgg attccggact
     6361 gatttggaat atgcgatgat ggcattgagg cttgccagga agcctatgat gaccgatgca
     6421 tgaaggacat ggccaatcca gcgatgctct cctaaatatt caatgggggg catcggctag
     6481 atttgttttt tcatgtagat ttcctcgtct ttgtatttgg aagggcattt cagcagtact
     6541 tcgttggcta cgaattcatc ccctttcatt tccccggtga ccacgatctg ttccgaccgg
     6601 tcgaaatcct ggggttttcc ctgaagcagg attactttct tttcagcgcc tttgggatcc
     6661 cgaaggtaaa atgaaaaata gtttgggttt tctgccggat tgtaatacat ctccttatcc
     6721 ttggcaaggg tgcccgcgac cttgacccgg tgctggtttt cggtcgcctc tgcaaaggtg
     6781 gcataggtac tcacatccct ggaaagggaa aagaataagg ccatgcccgt aatcaatacc
     6841 aaggccgcga tgatatgaag tttattcatg gttcaatgat ggacaaaagt acacgaagtc
     6901 ggctgcaaaa tcaaataaag tgaaggtcgg aagcttacaa atcggaaagt ttattatttt
     6961 tttcccgcct atatataaaa tgcaggaaat gtggctatta ttttgtaatt tagcaagcat
     7021 atccaatcca aatatgcgct ggagaaaatt cgacggccag atcatcgaag tccctatcct
     7081 ggaagcggtt gaaaaaacgc tggtcaggga atctgaagcc ggtaaccggc tgaaagtctg
     7141 cataggtacc gactccgagg tcaggcagga cggcattgaa tatgccacgg tgattgtatt
     7201 cctgagggaa aaaaagggcg gtttcatgtt tatctgcaat cagcaccggc ccaggatcat
     7261 gagtgtcaag gaaagaatga tcacggaggt atccatgtcg gtcgaaatcg cctatcacct
     7321 ctgcgacctc ctggaccggt acggagtggg gctggaagtc cacgccgata tcaataccga
     7381 tcctcatttc aaatccaatg aggccctgaa agaagccatg ggttatatcc tggggatggg
     7441 atttgttttt aaagctaaac cggacgcctt tgccagcagc tattgtgctg ataaagtagt
     7501 ttaaccaatt aaatttaagt tattgaggat caggctataa tcatatatga cctgataaga
     7561 aagtgccgaa ggtgggactc gaacccacac atcctcacgg acacacgccc ctgaaacgtg
     7621 cgtgtctacc aatttcacca cttcggctta gtatttcaat aaaaagggac ttcaccgtcg
     7681 cgaaagtccc tggccgattc agtaccgaag aagggaatcg aacccccaca acattgctgt
     7741 tactggattt tgagtccagc gcgtctacca gttccgccac ttcggcaaga aatcctgatc
     7801 caccggccag tcttttaagg ccgctgatct gccgcaaatt tattcaaatt ctcccgtaaa
     7861 cgcaaaatat atctgcgctt aaagtagtat tccttcaggg attccagttc tgccccggag
     7921 gccttgtcaa aatcaaaatg gaggatcagc ggatcgatct ccgcgcggat ggaattttct
     7981 gccgcttcga gttgcagatc tgcctttacc ggatcttcca ggataagatc atggatatcc
     8041 atcatttcca tcaaaaattc ctgggaaagt cctgattggt ctccttctcc ttccccggtc
     8101 agccctttta attccaggat gtatttaaaa agcaggtcgg ggttggaaag cgtccggtag
     8161 gccttgttgt taaaagcgct ccattcttcc gcttcctcct tttccagttc cggttttaaa
     8221 acgaaatggt ccggatgatg ttttctgctg gcttccagaa actttttttt aagaagttcc
     8281 gggtccgggg caaggctggg actcagtccg taaaattcaa aatagttcat ttcttaagga
     8341 aacggaacac atcaaggcca ttggcgtaga tcatcagggc tatcagtaaa ataaaaccta
     8401 ccagggtagc tttttctatg aatttgtcgc tgagcttttt tccggtcacc agttcaaata
     8461 gcagaaagac cacatgtcct ccgtccaggc cgggaatggg aagaagattc ataaaaccga
     8521 gaatcaggga aaggatggcg gtaatgcgga taaaatcctc ccagctccag gtatccggaa
     8581 agaggctggt gatgctggcg aaaccgccca ggctttcgga agctttgatc ttacctttga
     8641 acatctggcc gaaagctttg atctgatcac tcaggaagcc aagcccctta tttattcctg
     8701 ccggaatgga ttccatgaac ccgtatttct tcctttccac ggaaaggacg ggaagggtgt
     8761 atgccccaat cgtgccttcc gcggaagttt taaaagtgaa aggcaattcc tgccctcccc
     8821 gcttgatcac cagggtgacg gagctgtcct ttaattgccg caaggttttg gtcaggtcat
     8881 tgataaacat aggggtgccg ttgagggaga cgatttcatc tccgactgcc aggcccgcgg
     8941 cttgggccgg ggaggactca gaaaaccggt cgattttgac cgggacccgg ggtccgatca
     9001 gggtggagtt cttgttttta ttgctggaca agtcgtgtat aaatcggtct tccatgggaa
     9061 ggtcaatgac ctgtccgtcg cgttccacag ttattgtttt agccccattg atgaccacct
     9121 cccgccggag catgcgatga tcaaagatgt caaagtcgac ggcgcctacc ttaaggattt
     9181 tatccccgtc tttcagtccg atcacgcggc ccagggaatc ggtaaaaatg ccgtttggta
     9241 cggaacggga gggaaggtat tcttcacccc agatccccaa taccagggcg aaaagcagga
     9301 aacccgcgat aaaattaacc gtcactcccc cgagcatgat gatcagcctt tgccaggccg
     9361 gtttggagcg gaattcccag ggctttggtt cttctttaag tttttccgta tcaaaacttt
     9421 catccaccat gcctgcgatt ttcacatagc ctccgagggg cagccatccg atcccgtatt
     9481 cggtatcccc ttttttggtt ctgaacaggg aaaaccaggg atcaaaaaag aggtaaaatt
     9541 tttccaccct ggttttaaac catttggccg ggaaaaaatg tccgcactcg tgaatgatga
     9601 ccaaaaagga aaggctcagg atgagctgga tgatcatgat ggggataccg gtcatttaag
     9661 attatttgtt atgcaaaagg tgcaaaagta caacctcttg catccactaa caaactgccg
     9721 tccgccttgg ttctgtctta aggttttctt gaccaaaagc ggcaaggttg aattttgtat
     9781 tttgccctgt gcattatttt tttggaaatg cagggccggg gagcatcgcg gacctttctc
     9841 ccgaagaagc ccgccatgct tttgtcctcc ggctcaagcc cggtgaaaaa gtcggtttgc
     9901 tggatggaaa ggggacggta tgcctgggcg aagtgatctt cagcggcccc aaaggcgccc
     9961 aggtcctggt ccgcgaagtc aggaatcaac aggcgccaaa gccgcatatt cacctggccg
    10021 ttgctcccgt gaaacaatcg gaacgaatgg aatggctggt cgaaaaaatc acggagatcg
    10081 gggtctccga aatcagcccg ctgatctgcg accacagcga acggtttcac ttccgccctg
    10141 accggcttga gaaaatagcc catgccgcag tgaagcagag cgggagaccc tggcaggtcc
    10201 tgcttcatca acccgtccgg tttgaggctt ttatgagcca ggacttccgg ggcgcccaat
    10261 tgatcggaaa tcaaaatggc aggcccctca aaggcctttt tggggaagcg cggcctgacg
    10321 agatcacctt atgcattggt cctgaaggcg atttttcaga gcatgaactc gccctggcag
    10381 ataaaaaagg gtttattaaa atttcattcg gggatgtgcg tctgaggaca gagacggcag
    10441 ccttggtcat agcagggata ctgaccggtg gataaacccc gtgatcaaat gcgggcttca
    10501 tggtcttgtg ccccatctgt caccgtcctg caaattgtca tacacccggt gtttactgcg
    10561 tatatatttg tattaaaatt gaccatgaag atccgtgcat attctgtttt agtatttctt
    10621 ctgactcttc tgtttttctc cagtgctttc cgttttagcc aggcaccgac cttaaaactg
    10681 gccttgctga aatacaacgg aggaggggat tggtatgcca atcccacttc attgccaaac
    10741 ctggcccggt tctgcaacca ggctttgggt accaatctgg atgacgacta tgctacggtg
    10801 gaagtaggaa gcgcggacct ttttaatcat ccctttgtac acatgaccgg gcacggcaat
    10861 gtggtgttca atgatgcgga atccgaaaat ctccgcaact acctgcttgc cggaggattc
    10921 ctccatattg atgacaatta cgggatggac ccttttatcc ggccggcgat gaaaaaagta
    10981 tttcctgaat tgtccttcat cgagctgccc tttgaacacg agatctatca ccagaaattt
    11041 tctttcaaaa acggactgcc gaaaatccat aaacacgatg accgctcccc ccagggtttt
    11101 gggctttttt gggaaggccg cctgatctgc ttttattctt acgaatgcga cctgggcgac
    11161 ggctgggagg accaggaagt gcataaggac cctgaagaaa caagattgca tgccttgcaa
    11221 atgggggcca acctggtgca gtatgctttt aatgaatcga agtagggaga aaaggagaaa
    11281 gggagaaagg gaggaaggga tttactccca atggaagatg tggttgttct tgcggatata
    11341 tcgggtgata ttcagccaga aggccaggat aaagtagacg ataaccgggg aacccagcgt
    11401 aaaaaaactg agataaataa aatgcaggcg gatcttggta ttgtctatgc caagccgctg
    11461 tcccaggtag gtacagaccc cgaagactga tctttcaata aaagatttga acatgagtgg
    11521 gctaatctga tgtaaatttg cgcctttaag ttacggacaa aatgaataaa gaaaaagaga
    11581 tcaagcagga aataaatatt gagctgagcg aagagatcgc cgaaggcatt tattcaaacc
    11641 tcgctatcat atcccattcc cacagcgagt ttgtgatcga ttttgtcaga ttgatgccca
    11701 atctgccaaa agcaaaagtc aagtccagaa tagtactcac tccgcagcat gccaaaagac
    11761 tgatgatggc cctcggtgac aacataaaaa aatacgaaaa tcagtttggc gaagttggag
    11821 acgacagcga ttttccggga ctgcctccca tgaattttaa tacgcctact gcacaggcct
    11881 gaccttatat ccactgattt tatccatatc attgatcctc aatggactgg aaagccgtct
    11941 tagaaaatga agtatccctg gaccctgagg attgggaagg agccaaggcc cggggccggc
    12001 aaatgctgga cgacctggtg gaatatctga gacacatcgg ggacgaaccg gtctggaggc
    12061 agccgccccg tgaaatgaag gattttttac accacaccct gcaggattcc ggccaggatc
    12121 ccgatctgct gtatgcccag tttaaaaagt atatcctccc ttatcccaaa ggcaatattc
    12181 atccgaggtt ctggtcctgg gtacagggca ccggcagcct gaccgcagtc tatgcagaca
    12241 tgctggcttc cgccatgaac tcaaacctgg ccatcgggga ccatgccgcc atgtatgtgg
    12301 agcagcaagt gcttgattgg tgcaagtcca tgctggctta tcccaaagaa gccagcggga
    12361 ttatactcag cgggggctcc atggccaaca tcaccggatt gacgattgcc cgcaacgcat
    12421 cggacgagag gatacgttcg gaaggggtgg gcatttccgg caaaccactg gttttttatg
    12481 cttcggaaga aacacacagt tgtcagcaga aggcggccga ggtcctgggc attggttcca
    12541 aagggttgag gaaaatcccc gtggacgacc aataccggat gaggatagac ctgcttgaaa
    12601 aacaaatcga agcggattta aaaaaggatt gcctgccctt ttgtatcgtg gccaatgtcg
    12661 gcaccgtcaa taccggagcc attgatcccc tggaagaaat ccggcaaatc tgtcatcacc
    12721 ataagatctg gttccatgta gatggggctt tcggggcgct cgccaaatta ttgcccgaat
    12781 accaggattc gctgaaagca atggaacagg ccgacagcct tgcttttgac cttcataaat
    12841 ggatgtatct gccttatgaa atcggttgtt tgctggtaaa agacaggcaa aagcaccggg
    12901 atgcctttgc ccttcagccc cattacctga gcagccatga acgagggttg ccggccggtc
    12961 cggatcccgt gggcaattac ggattggaac tctcgcgggg atttaaagcc ctgaaagctt
    13021 ggttttgttt ccaggaatac ggattggata aataccggca aatcatcagg cagaatatag
    13081 cccagtgcct ttacctggcc gacttggtcc ggaattcgga tttccttgaa ctgttggcgc
    13141 cggtgccatt gaatattgtt tgtttccgat ttaaagtccc gggcatggac gaggaggcac
    13201 tgaaccggct caataaggag atcctgatgc gcctccatga agaggcggtg gccacgcctt
    13261 cctacaccat tttaaagggc agatatgcca ttcgggtagc caatgtaaac caccgaagca
    13321 agaaacatga ttttgacgcc ctggtggaag gggtggtccg tatcgggcgg gaactgatgg
    13381 ccgctatcaa acccggctga caagattcct gttcagaccg ttttgtccag gctttttcgc
    13441 atggaaggcc aggaagattt atctgaagcc accagcccgg aaacaaattc acccatcgcc
    13501 gaacaggtga tgatcgtcct tggcgccata tccatggtac ccaggccttc attgatgaga
    13561 aaatcaacgg cttcacggat caacccggcc tccgcggtca aatcaaaaaa atcgagcatc
    13621 atggccacgg acaggatgga agcaatgggg ttggcgaggt ttctgcccgc cgcctgggga
    13681 tatgaaccat ggataggttc aaagaggccc tggttttcat taagggaggc ggaaggcaaa
    13741 agcccgatgg tgccaacaac cacgctggct tcatctgaga ggatatcccc aaacatgttc
    13801 tcggtcagga tgacgtcaaa ttgccttgga ttgagcacca gctgcatggc ggcgtggtca
    13861 ataagcatga gttccagctc tacatcggga tagtctgatt tgtgcaggtt ttgtaccgtg
    13921 gaccgccaca gttttcctgt ttcaagcacg ttggccttgt ccaccagcgt aagctttttt
    13981 ttcctcagcc gggcttgccg gaaggccagg tggcttattc tttctatttc ctcctggtga
    14041 taataacatt catcaatggc ccagcgtcct tcttcgtctt tctgtttttt cccgaaataa
    14101 aggccggcag tcagttcccg gaaaaggatc aggttgatgg aatgcacctg ttcctcctta
    14161 aggggtgaga gggcagccag tttacggtag cttttgatgg gccggatatt ggcgaaaaaa
    14221 cccatcgatt tgcgaaggtc cagcaatccc tgttccggtt tgaagaccgt cccgggatct
    14281 ttgtcatacc tgggatcgcc gatagctcca aaaaggacgg catcggcctt ttggcagagt
    14341 tccagggtag cgggaggaag gggctcaaga ccctgggcca tggccgccgc gccgatggga
    14401 gcctccgtac attcaaaccg gtgctggaat ttattttcta tgactttaag aacttttaag
    14461 gactgtgcga tgacttccgg tccgatcccg tcgccaggta aaacggcaat ggttttattc
    14521 atagtctaaa aatgaggatt gtcagggata aaacccctgg gcggaatgat tatatttttt
    14581 aatgaaaccg gttgcggata tccatggata ggctgcccgc tcaaaccacg gctttgtgtt
    14641 tttttacaat gtgttcatcg tagagccgga gcaggtcctc cgctacgatt tctttcttct
    14701 ggtcggccag ttccaggaaa tttttataaa ggatatcgag ctgttctttt cccagttcga
    14761 tgtccaggtt gcggtagcgg taggcgaggg cggccctgcc gcttcgcgcg gtaaggatga
    14821 tggcggattc attgaccccg acttcatggg ggtcgatgat ctcgtaattt tcccggtgtt
    14881 tgatcactcc gtcctggtgg atcccggagg aatgggcaaa ggcgttggcc ccgacgatgg
    14941 ctttgttggg ttgtaccgga atacccatcc ggtcggagac catgtggctc atttcggtga
    15001 gcattttgct atggatgttc gtgaagaagc cgaggtgttt gcgctgcttg atgatcatca
    15061 ccacctcttc cagggaggta ttgcccgccc tttcgccgat gccgttgatg gtgcattcga
    15121 tctggcgggc cccgttgcga atggccgcga tggaattggc cgtagccagc cccaggtcat
    15181 tatggcaatg cgaagagagt atggctttgt ctattccttt gacattttcc ttaaggtacc
    15241 ggatcttgtc cccatattca tcggggagac agtacccggt ggtgtccggt atattgagta
    15301 ccgtggcgcc ggctttaacg acttcggtac ataccttggc cagaaattca ttttcggtac
    15361 gcccggcatc ctccgcgtag aactccacat cgtaaacaaa ttttttagcc agcctgaccg
    15421 cggccaccgc ccgctctatg atcttttcgg gggtgctgtt gaatttatat ttgatatgta
    15481 catccgaagt gccgatgccg gtatggattc gcggactccg ggccggctgc agcgcttccg
    15541 cggccgtgat gatgtctttt tccacgctcc gggataaacc gcagacggtg gaatgtttga
    15601 tgatttccgc gatctggtat acgctttgga aatccccggg gctggaaatc gggaatccgg
    15661 cttcgatcac gtccactcca agggattcca gggcttccgc caattccaac ttctgctggg
    15721 tattgagttt gcagccgggg acctgttccc cgtcccgaag cgtggtatcg aatatttgta
    15781 ttttttcggc catgaataaa ttactgatat ttgtcctgtg gtcgcatttc tgcaaaagac
    15841 agttaaaatt attgcaaata ttacggacgg acaaattgca gtttacaaca gctacgaccg
    15901 gacttcaggc catgaaggga taaactcttg attattaggt tttaaaatcg atttgtacta
    15961 caatgcccaa acaaccgacc gacttccttc tgcaattgat aaagtccatg tccaagccag
    16021 aaaagaggca ttttaaactg tttgtgcagc gcaatccttc caatacggat tccctgtttc
    16081 tccgcttatt cagtcatatg gagaaacaaa gggcctatga tgaaaagagc atcctccacc
    16141 agatcccgga aattaaaaaa caacaacttc caaacctcaa agcccacctt tacaaacaat
    16201 tgctggtcag tctgcgcctc atgcaccggg gagaaaatga ggatatcgac gtccgggaaa
    16261 aaatagacta tgcgcgggtg ctgtacaaca aagggctgta ccggcaggcc ctggacatgc
    16321 tggacaaagc caaggaaaaa tgcggcacct ttcagttgca ttccctccgg atggaaatcc
    16381 tggattttga aaagttgatt gagtcccagt acattacccg tagcatcgca tcccgggcgg
    16441 agcagttgtc cgcggagtcc cagtatacgg ccggagaaat cgcacgcatc cagcaatatt
    16501 caaacctgtc gctccaattg tacagtctgt acctcaaggt gggatatgtg cgcaactcca
    16561 aggatcattt tttcgtccgt gaatttttcc aggcccactt gccggtaaac cgggagcagg
    16621 acctgggttt ttatgaaaag gtctatctgt accagtctta tgtatggtat taccacatgc
    16681 tcctggattt taaaatgtat taccgctatt cccagaaatg ggtggaattg tttgaccatc
    16741 atcccttgct ggccgccatg gaacgccccc ttttccttaa agcccggcac aaccagctga
    16801 atgccctctt cctgctccag cggcacgaca agttcatgga agcgcttcac aagctcatga
    16861 cggaacttaa aaaacaaccg gccagaaatg acaatgaaaa gagccttgaa ttcatatata
    16921 cctataccca tcgcatcaac cagcattatc ttgaagggac ctttcagcag ggattgccgc
    16981 tggtccatga aatcatgcat catatcaagg cggacaccca tcacctcgat ttccaccgcg
    17041 tcattctctt ttattataag atcgccgcgc tttattttgg cagcggggac aacagccggg
    17101 ccatcgatta tctcaacctg attgtcaacg aatccccgga cttccggggg gatatccaat
    17161 gttatgcgcg catcctgagg ttgatcgcct actatgaaat gggggataat tacctgatcg
    17221 aataccaggt gaaatcgatc taccggtttt tgaccaagat ggacgacctg cacgccgttc
    17281 aaaaggaaat cctgagcttt atccggaaat tacccagggt ccaccaggcc gatctcaggc
    17341 gggaattcag gaaactgctg gataaattca tccgcctgcg cgccgatcct tacgaaaccc
    17401 gttccttcct ctacctcgat atcatttcct ggcttgaatc aaagatccag ggcgtaccgg
    17461 tgcaacaggt catccgagaa aaatttctcc gtaaatacaa ataaccaatg gcccgcaatc
    17521 attcccggtt gccgtttttc cagtcaaagg aagccaggta ggccaggatc agccctccaa
    17581 aaagcaggat cagttttaac aacaaaggaa aaatccaggg cagccggtcg atccaggcca
    17641 ggtaagaaaa ttccagtccc aggagggaaa gcagcagggt aagaaatcca aaaaggaagc
    17701 agaggaaggc tcccgtcaat atccattgtc ttttattcaa agcttcagag attggggcaa
    17761 aaatacaggt attgacgcac aaaggctccg gggtctttcc ctttccttca tccgtatgcg
    17821 tccaaactca gcgccaggcg cctacgatgg ctccagccag agccagaagc aacacctggt
    17881 aagccgcgtc gatcagccac aatttgaaac ttttgcgctg gtacaggtag tttatcgcgg
    17941 ttgaagtagc cacaaacaca agaccggtca ccaagcccat ttgcaggcct ttcatggcat
    18001 tcatcgcagc atccccctga aaaaacatcg ccaggccggc cgacatgatg atcatcatga
    18061 ccgcactggt tccgaaaatc aggggcatgt ttgcattttt gagattttct tcagtcagcc
    18121 cggattcttt ttgccaggcc tttccgaaaa ggacagggct gtaccacaat gccccgagcg
    18181 cccaggctgc aatgccggcc accaggaccg ccagccaatt catcccgttc gattccataa
    18241 tttagaaaaa ttgttttaag tgaatggagt ttaaaagggg gaatcccggt cgtttgttca
    18301 acccgataaa tttaaccatt ccccaatgga tttccagtat aatcaaaacg gtatttattg
    18361 cctggtcgaa atgccggttg agaattttcg ccgatcttta ggcctgaaaa tggtcaagct
    18421 tatagaatgt ccgcgggatg ccatgcaggg ccttaaaacc tttataccca cggaaaaaaa
    18481 aatagcgtac atcaatcagc tgatgagggt gggctttgac accattgatt tcgggagttt
    18541 tgtttcacac aaggcggtgc ctcagatggc agatacccat gaagtgatcc gtcggctgga
    18601 gcctgaaggc aagtccaaat tgcttgccat cgttgccaac ctcagggggg cggaggatgc
    18661 ctgccggtac gaggggatcc ggtatgtggg atatcccttc tcgatctcgg agactttcca
    18721 gaggaggaat accaacagta ccattgcgga atccctggaa accgtgaagg agatccagga
    18781 tttatgcgcc caccacggca aacagctggt gatctatatt tccatgggct ttgggaatcc
    18841 ttacggcgac ccctggaacg tgcacattgc gcaatactgg gtggatgagc tggctgaact
    18901 cgggatccgg atcatttcct tgtccgatac cattggagtg tcaacgcccg attccattca
    18961 ttacctcttt tccaatctta ttcctcctta tccggatgtg gagtttgggg ctcatctgca
    19021 taccatgccc cggagctgga gggaaaaagt ggaagcggcc tggaatgggg ggtgtaaacg
    19081 tttcgacggc gcactgaaag ggatgggggg ttgcccgatg gccaaagacg atctcaccgg
    19141 caatatgccc accgaacgaa tgctctcctt ttttaaagaa acggtccctg atctttccat
    19201 tgactggacg gagctggaca aagcgatggc tatggctgat acgcttttct attcctgatg
    19261 ccgcagaaat tcaaggatgt actggtcttc catggatttc aattcctgca gcaacttgcc
    19321 agccggatcc caacactcgg tactggaaaa tacatgttca tcaatctgac tgaccggccg
    19381 gatttttttg gtggtagagg tcaggaacac ggatttacaa tgcagcacct cctgcaggga
    19441 caggggacgg atctccaccg gcatataggt tttggcaagg cggatgacat ggtcccgggt
    19501 cactccgcca aggatttttt cagagggggt aatgaggata ttatcgtagg ttaccgcgaa
    19561 cagattcgcc ctggaggttt cagaaaccca tccatcgtga acgtataaca aatcgtgaaa
    19621 gccggctgct ttgctttctt tccagtgcag gaggggcacg aggtaattca atgattttac
    19681 accgggcgtt tcccggacat aatcaatggt ctttaagcgg aaaggatgga tgtctccttc
    19741 ttcggtcacc gggaagatca gatcgccggc aaggatcatc aggcggtttg ggccatcggg
    19801 gtcaaagccg ttggaagaaa tgccgccggt gaggaccact ttcatacttc cggtgatcat
    19861 tttattttta gcgatcagct caataatatg ctttcgaagt tcttttattc cataggggca
    19921 gaccaggtcc acctgtcttg cggaatttaa aaaacgagcg aggtgttcat tcaggaacat
    19981 ggggacgccc tgtttgacat taaaaaaatc aaacactccg tacccgcgaa gcaatccgag
    20041 atcaaaaacc ggaatgaggg cctgggaatc aggaatgatc tctcctccta taaagtggta
    20101 tttcatttta ttcgtgattg ggtagcttta attgacctga ttgagtccga taataatgat
    20161 atgcggctgc agccgctgca agatatttat cctccaggtc aggcgggtat aatatttcat
    20221 tcagatgctg gtcgagaaaa ccggtggaca agtcgccggc cagcaccgcg ggatgttcca
    20281 aaacccggat gccaaaggga atggaggttt cgataccctc tatgataaac tgttgaagcg
    20341 cttccttcag ccgttccatg caggaaaaac ggtcttggtc cgaggcaatc agtttggcga
    20401 tcatgggatc ataatacact tccacctcac tgccctggcc gtatcccgaa tcaatccgga
    20461 tctgcggcag ggtgggggcc aggtattttg tgatttgccc ggttgaaggc ataaatccct
    20521 gcgcaggatc ttccgcgtaa atacgccatt gcatggcatg cccgactgaa cggatttctt
    20581 cctgggtaaa gggcagggac cttcccaaag cgatatcgag ctgaaggtcc accaggtcca
    20641 gcccggtgag catttcggtg accgtatgtt cgacctggag gcgggtattc atttcaagga
    20701 aataataatt ttcttccgca tccacgataa actccaccgt gcccgtattg gtataaccgc
    20761 aggcctgcgc caggcggacc gcatccctcc gcagcgcctc ccgggtggtg cttgaaataa
    20821 aaacgcaagg ggactcttcc atcagtttct ggtgccgccg ctggaggctg cattcgcgct
    20881 ccggaaaagc aatgcaacga ccggaaaggt cagccatgat ctggacttca acatgccttg
    20941 gattttcaat atatttttca aggaatatgc tgtcgtccgc aaaagaagct ttggattcag
    21001 ccctggcacg gctgatggct tcgacgattt gggacagatc cttcaccacc cgcatgccct
    21061 tgcctccacc gcctgcagcc gccttgatca tcagtggaac gccgatatgc ctggccatgg
    21121 caattccttc cccgtcgtcg cggatcggcc ggtgatagcc ggggaccagc ggaatgccga
    21181 gttgttctgc gatctgtttt gcggcaatct tacttcccag tttttgcatg gcctcaaccc
    21241 ctggcccaat gaagaccagt cctgcggact ccacggcgct cacaaattca cttttttcac
    21301 tcagaaagcc gtagcccgga tggataaaac cggcgcctga ttttttagca gcctccagga
    21361 tggatggaat attcaggtaa ctgtctttgg ccggtgcagg cccgatgcaa taagcctcgt
    21421 ctgcatgggc cagatgcagg ctgtgccggt ccgcttcgga atagacggct acgcatttcc
    21481 agcctttttt tctggctgta cggaaaatac ggatggcaat ctccccccgg ttggctacca
    21541 gcaattttgg cataagcggt taaaatacaa tatctttcct ttctattcac ctctgctatg
    21601 gattccaaaa aaatatttga cccgcccccg gaatggatat cctcgtgcaa tatgaccgcc
    21661 tatatcagct ggctaaaaaa aaacaaaaag ctggactttt cggactacga gtccctatgg
    21721 aaatggtcag tggaccgggt cgaggatttt tggtccagta ttctggaata tttccaggtg
    21781 cagtataccg gcagttttga gcaggtgctt tcctcccgaa aaatgcccgg cgccaagtgg
    21841 tttgaaggga taaaactcaa ctatgcagca cacctgaccc gtaaggtaga actggaagct
    21901 tccgccctga tcgcctgcaa cgaatcggga cagatccgtc cttatacctg gcaaagggtc
    21961 ctgcgggagg ctggggcagt gcaaagggca tttaaccggc atggacttcg gcccggcgac
    22021 cgggtggccg cttacctgcc caatatcccc gaggcctcca tctccttgct ggccatcgtg
    22081 gccagcggcc tggtttggtc ctcctgttcc ccggagtttg gcagtgccag tgtcattgac
    22141 cggatgagcc agatacagcc cagggtattg attgccgtgg atcattaccg ttattcggga
    22201 aaaacgttcg accgcagggc ggagctggaa gccatcgtga atggcatccc cagcctggaa
    22261 ttagtgatcc tggtggatcc gttgcctggg ggaagcggag aacttcgagg gttgcctgtc
    22321 ataccctgga cagctgttgc aggggaggaa ggggagttcc ctgaatatgt cgaagtacct
    22381 tttgatcatc cgatgtggat cctgtattct tcgggcacca ccgggcttcc gaaggccatc
    22441 acccattccc agggcgggat gttgctggaa catctcaaat acattcattt tcacaacgat
    22501 gttcggaaag gcgaactgtt ttactggtat tccaccacag gttggatgat gtggaattac
    22561 cttcatacct cttggctggc cggtgcggtc ccggtgctgt atgacggcca tcctgcccat
    22621 cccggattcg ccagattgtg ggacctggcc agggaccttg gcatccatca ttttggaacc
    22681 agcgctccat tcatccacag ttgcatgaaa gagggcttgg ttttgccgga attaccacta
    22741 ctccggagca tcagcagcac cgggtctccg ctgagtccgg aagcctacga ctggttgtac
    22801 cgggagatag gacatcccct gtatttatgg tccatgagcg ggggtacgga tgtatgcacc
    22861 gcctttgtgg ggggttgccc catatccccg gtttatgaag gcgaaatcca gtgccgagcc
    22921 ctgggctgtg accttcgcgt ttacgatgaa caaggacagg aattaaacaa tgaggtcggg
    22981 gaattggtca ttgtccaacc catgccctgc atgccggttt atttttggaa cgatccggat
    23041 ggccataaat accacgaagc ttatttcgaa gtattcccgg gggtctggag gcatggagac
    23101 tggattcaat tgacctcaac agggggcttg gtcatttcgg gacgttcgga taccacctta
    23161 aaccgtcttg gggtccggat cgggacggct gaaatttatg ccgccgtcca gcaactggag
    23221 caggtggagg atagcctgat catccatatt gacctgaaat cgggggaagc ctacatgccc
    23281 ctctttgtca agttaaagca cgggcagttg gaggatcatt tattgagtga aatccgggca
    23341 accatcaggc atcattgttc accccgtcat gtcccggatg caatcatcca ggtcaaagac
    23401 atcccctaca ccctcagcgg aaaaaaaatg gaaaatgcgg tgaagaaaat ttttcaaggg
    23461 aaggagttgt ccctggtcgc ttcacccgga gccatgcgga acccggacag cctggaagaa
    23521 taccaggaga tcaggaattc actggactta aggtcctaaa agatcccgat gatcaagaag
    23581 gccaggaagc ggtgttttta aatcaaaccg gaagtcccta tattgaagga gactatgacc
    23641 aggcggcctc aaatactgac catcgaatcc ctggaaaaat ttgagcgggt gatcaaccgg
    23701 gacttccggc cctatgacat caatatgatg gtcatgcccg gtaccctgga ggacaagcat
    23761 aaaaactgtg cctccattcc ggccctgcga agacagttta acctgatcta tctgcttctc
    23821 ggaggtgaac acgatgtccg gctcggggca gaacataccc gtctccggga ccacgacctg
    23881 gtcatcgtgc ctgaaaacac cgtttacgcc agcgaccata tccgtcattg caccgggttt
    23941 tgcatccatt ttaaaactga atttctccag accctgctgc agcgtccttt atcggaagag
    24001 ttcccgtatt tccgcatgga tgcccatcat attctcggcc tcagcccggc cgaaagcaag
    24061 atcatcaggc aatcttttga aaacatcatc tcggaatatc agcggttttc ccccgagaag
    24121 gaatatattc ttcgcaacct gatcctgatc cttctactgc gggtgcgtga aatttaccgg
    24181 acccatacca aaaccatccg tcaaaatacc agccgcccgg agcaactgtc cacccggttc
    24241 aaacagctgg ttgaaaaaca cttcaggacg gagcgcaccg tgagcgagta tgccgcccgg
    24301 ttgggtgtga cctccgggca tttatcggac gtggtaaaga agaccaccgg aaggtcgccc
    24361 cgtgaagtga tccaggatat gcttttccta gaatcaaagg tgctgcttgc cgcaacggac
    24421 aagaccctgt ccgagatttc cggggagttg aacttccggg acctggcgca tttctcccat
    24481 tttattaaaa aaaagacggg tcattttccc caggccttcc gtaagcaact gtctgtctga
    24541 atgaaaagaa aacgggccag ggtctccatc cgttaattcc tgtttttata caaagaatgc
    24601 cgaagttcct acaagtccgt caggtgttgc cttgatcaac tttgtatcat tcattcattc
    24661 ttaaaaaatc ataaatcatg aaattaaaaa ttatgctgtt ggccatgatc gccaccctct
    24721 tcatggctgc acggatgcat cacgccggag ttgctaaagc cgaacacaag atgttcgcca
    24781 aaccggatga agtaaggacc tttccgcttg gtaaactgga acttgtgaaa ctgggtggtg
    24841 ccatgatcgg cagggcgacc tttcgctcgc ggcgccattg gacgccggcg gcgccg
//
LOCUS       DAOQXT010000071        24361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2405_length_24361_cov_5.630379, whole genome shotgun sequence.
ACCESSION   DAOQXT010000071 DAOQXT010000000
VERSION     DAOQXT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24361
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_24361_cov_5.630379"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="PKM27_12680"
     CDS             <1..1694
                     /locus_tag="PKM27_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR08166.1"
                     /translation="SAAHELIERGFQVEVYEANPVYFGGKARSEDHTGISANPLPGEH
                     GFRFFPGFYRHITHTMKRIPFGAGTCYDNLVTTERILIARNGQEPIKIQAEFPFTAAG
                     FNLVLSTLSGADTGLTGPEKKLFKNKLFQLLTTSKERFESEYEQQAWWYFVEAHQNSP
                     NYQNLLADGLTRSLVAAQAKKMSTRVGGGILLQLIYDIVDPGIPADRVLNGPTNQQWI
                     QPWISYLQAKGVQFFLNHTASALNVDAVGRLQSVRLDNPATGIPVYATGDYFILAVPV
                     EKAKDLLDHPVITNRDPIMSFPTELSKSVRWMNGIQFYLSTEIPLNPGHIIHIHSPWA
                     VTSISQVQYWSGYDIDSQSGGLVKGVLSVDVSDWQDGIYNGKRAKDTSEAELIQSTYE
                     QIQQSLIVGGVPLLPANRADVVVGYHIASSIRRTGPTQAVSDEPLLVNTVNSWDLMPK
                     SFTGIPNLFLAGDYVRTFTGLATMEAANESARRAVNCILSASGTGKPYAKVWRLREWA
                     MVLPYKWWDRRRYSKGLPYSSGFPWWLHVIAFIWSIICLVAWAIGLPFRWLFGFI"
     gene            1707..3032
                     /locus_tag="PKM27_12685"
     CDS             1707..3032
                     /locus_tag="PKM27_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR08167.1"
                     /translation="MLNKLKAWLDSLIIRHLPDSGHHKQARMIVRGSLLTVLFMAYYA
                     LISKVISYKEGVLIQSILTLGFALIPLAVLRRANLNLIGNLFVFSGCTGIVMLSLHTG
                     GLESPVLPWIGLVPIVALLFTNRNWAWAWFVLTLAVVALFIYLKYQGMIPSPRFDTSY
                     KVFLAANCLIGLVLTLFLLNTIFVNSIREINQQLESKNQEIEEKNKLLEQQAGQITAQ
                     RDQIILEKQKSDKLLLNILPADIAEELKQNGSCEARQYENVSILFTDFVNFTNYSEKM
                     SPQSIIAELHRYFTAFDAIIEKYGLEKIKTIGDAYMAVSGLPERNSNHAVLAILAAKE
                     IREFVQASMNPEGRSSENSGEGKQGFQARIGIHSGPVVAGIVGSKKFVFDIWGDAVNT
                     AARIEQNGFPQKISISGQTYELVKDHFPCEYRGKVKAKNKGELDMYFVN"
     gene            complement(3040..4251)
                     /locus_tag="PKM27_12690"
     CDS             complement(3040..4251)
                     /locus_tag="PKM27_12690"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNR08168.1"
                     /translation="MPFRLTQLDIVPKFIYFILFLLFFQSACKPESDRGTLIEPLSES
                     DIVSADYIEPGIKWGYIDLDGDLVIKTQFDDCRNFSDGLSAASDKGLWGYIDRSGEWL
                     VKAQYRGAWSFSQNRGRIKILNGKYGFLDTNGILVLDTLWEDAEDFHEGVARVKKDNR
                     YQFIDLEGRLISEDSWDQAFGFNQGYARISNGGWWGILNRGGKMIIPPKYDAIGQVSE
                     NRIPVQVKGKWGYVDTLHELVIPAQFDEATAFQEGSAAVAKDGKWMLIDLQGRPWQGL
                     PRPGLDWNYIYPLGEGRWAVQKNDSFALMSPDGLLLSPFQYNQINAFHEGLAVFSKDD
                     LFGYMDENGRHAIPNIFLLAWDFHEGVARYSLGEGIGFINREGKSIHNGLFPDVRDYH
                     EGLARVQTVTR"
     gene            4252..4779
                     /locus_tag="PKM27_12695"
     CDS             4252..4779
                     /locus_tag="PKM27_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08169.1"
                     /translation="MAKLLLCCLLFPVFNPLAAQKPTILEPANCAVLIPGTFQYSADT
                     IPTEVGEIIYHTWHSRDSTVQYLVSFCNYPEGTVHSDSTELLEEFFTATISATVEKTR
                     GIKRYESGMLQSGFPGRFWRIDIGTRYVKTRAFVAGTRFYSLQVIGPLGAEQDKSESA
                     FFDSFRFLDLLRARK"
     gene            4845..5315
                     /gene="coaD"
                     /locus_tag="PKM27_12700"
     CDS             4845..5315
                     /gene="coaD"
                     /locus_tag="PKM27_12700"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNR08170.1"
                     /translation="MIIAVFPGSFDPITIGHVDVVNKALPLFDKVIVAIGTNTQKKSL
                     FSLEQRKQWIMDVFQGVDKVEVDHFDGLTAVYANRIRANYLIRGLRNGSDFDYEKTIA
                     HVNSTIGHGLETVFFISSPEVAHISSTIVREIIGGGGDATMFIPREIHLKPLTF"
     gene            5329..6957
                     /gene="pruA"
                     /locus_tag="PKM27_12705"
     CDS             5329..6957
                     /gene="pruA"
                     /locus_tag="PKM27_12705"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNR08171.1"
                     /translation="MSGNFKLPPVKNEPVPEYLPGSAEKLQLKLALAKAKSSTPEIKM
                     TIGGKKISTGPSIPVRAPHDHQQILAYHSKGGKEQVKAAIQASLNAKAAWQHTPWEER
                     AAVFLKAADLLSGPYRARMNAATMLGQSKNVFQSEIDAVAELADFFRFNVKYMTEIYA
                     TQPASPPGTWNRLEYRPLEGFVLAITPFNFTSIAGNLPGAPALLGNTVVWKPAETQIY
                     SAAIIMEILEKAGLPDGVINMITIDGPSLGEVAFSHPDFAGLHFTGSTGVFNHLWQTI
                     GNQLGRYKSYPRLVGETGGKDFVLMHASSDPKAVATALSRGAFEYQGQKCSAASRAYI
                     PKSQWKKVQEYLLDDLASMTIGEPENFSHFINAVIDEKAFDKISSYITGARKSKDASL
                     LAGGRPDKSKGYFIEPTVILANRPDYKTMVEEIFGPVLSIYVYEDKSFEDTLKWIDST
                     SPYALTGAVFATDRQVIQQTSRQLMQAAGNFYINDKPTGAVVNQQPFGGARGSGTNDK
                     AGSILNLYRWLSPRTIKENFNPPRDYRYPFMSEE"
     gene            6968..7516
                     /locus_tag="PKM27_12710"
     CDS             6968..7516
                     /locus_tag="PKM27_12710"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNR08172.1"
                     /translation="MKIYTRTGDEGMTSLFGGTRLPKHHVRIEAYGSLDEVNAWIGLV
                     RDQVSDENTAAVLKKIQDHLFAIGSHLAASPDKKLPLPEVTGEDIKLLEDEMDHMTTQ
                     VSPLKHFILPGGHKRVSEIHLARTVCRRAERRVMALHQESPVEPVILVFLNRLSDYLF
                     ILGRWTAHQLGIEEIKWNPRKE"
     gene            7495..8313
                     /locus_tag="PKM27_12715"
     CDS             7495..8313
                     /locus_tag="PKM27_12715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08173.1"
                     /translation="MESPQGMNLDLAAIDQKDLEGLKAFQPADWPDITKAYAFYIPSP
                     YCQPLKLMENGEIRAIGCAIRHAQTAWLAQIIVAEPYRNRGLGSKMTQCLIDLVKPEV
                     RSISLVATDLGAPVYTKLGFRPVAEYSFFRRAEPLPPNPESGVADLNSSILGQVLELD
                     LEASGEDRSLLIRDHLENGKVISSNSVLYAYYLPTLGEGLIISRDEKAGQELMRLKLS
                     ARLRHVIPEGNVTAKNFLLENCFEPYRKALRMVLGDPVHFKPEWIYSRIGGNLG"
     gene            complement(8320..8886)
                     /locus_tag="PKM27_12720"
     CDS             complement(8320..8886)
                     /locus_tag="PKM27_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08174.1"
                     /translation="MKKFISSNLILGLVLAGILSRLIPHAPNFTAVGATALFAGALIR
                     PRWMSFIIPLLILWLSDLYLNNGMYRNLFPEHYTGWQWMGNAWVYTGFVLIVALGQWG
                     IRKINVKNVLLSSILASLLFFMVTNFGVWINSPAWPQNPAGLLACYEFALPFFWNTFA
                     ADLLFSGVLFGSYVWVTRYIGQRLEVRG"
     gene            9035..9469
                     /locus_tag="PKM27_12725"
     CDS             9035..9469
                     /locus_tag="PKM27_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08175.1"
                     /translation="MKQGIYQWDDGYPDASTLWQNLEEGDTWIMRDGDVLTASVTLDR
                     NQDPQYEKIGWAYPSDRFLVVHRLCTHPGYQGRGLARQLAYFAEQQAQDLQLDVIRLD
                     AYLGNPVSQKLYRSLGYHEAYGYCYYPPRDIMCNCFEKWVGS"
     gene            9510..11357
                     /locus_tag="PKM27_12730"
     CDS             9510..11357
                     /locus_tag="PKM27_12730"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNR08176.1"
                     /translation="MKRLSILMVLCLGGGLIQAQPNRWQQQVSYKMDIDFDVQSHRFT
                     GQQTLVYTNNSPDVLDRVFYHLYYNAFQPGSVMDVRSRNLPDPDGRVRDRISKLTESE
                     IGYHEILSLTQDGIPVKYHIEGTILEVKLAKPLKSKGKTTLAMKFRSQVPVQIRRTGR
                     NNREGIAYSMSQWYPKLCEYDYQGWHANPYVAREFYGVWGDFDVKIRIDKKYLVAAGG
                     YIQNPQEVGYGYEKPGSQVKPPSGEKLTWHFKAPRVHDFLWAADPEYKHTRLVNDDGI
                     DMHFFFQENDKTRDTWSRLPAVMNQVFKIANKRFGKYPYSYYAFIQGGDGGMEYPMAT
                     LILGEGSMDGLIGVSTHELLHSWYQMVLGSNESLYAWMDEGFTSYAQDIILAEIEKPG
                     SADQYGTGACAGAYQGYFNLAKSGREEPLSTHSDHYESNFAYGVAAYSKGEVFLHQLE
                     YIIGKQAFQAGLLDYFNTWKFKHPNVNDLVRVFEKRSGLELDWYKEYFVNSTKQIDYA
                     VDSVYEENGQARIVIRKKKPFIMPLDILVSLTDGSQNLEYIPLDLMRGEKIQEWPDKK
                     LNLQPDWTWTYPSYTLKLKVPFSQVAGIEIDPSKRMADTDRENNRWVRK"
     gene            complement(11471..14542)
                     /locus_tag="PKM27_12735"
     CDS             complement(11471..14542)
                     /locus_tag="PKM27_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNR08177.1"
                     /translation="MSHPFVDRVCNHESSLITGMRYFFLLLFFQLATVHSRVPAKKTA
                     EIKPGPDFSIAEDSIRASTLFQHADSALFNLQYDSAIIYFEKSANQYQRIVDSTRDSL
                     IWAGYANALFSIGFCHQVNRDMKKGLEYLQQSLKVVLERFGEDHPTAAWTYFKMARSY
                     LAMGDGNAERSCTLKGFNICQRISGKYDFLLGQAYHEMGYQYFLLNDQKKIEIYFNKT
                     EQIYDSALARYGGGPAGSESYPFPTKPVHADGPQPNSVLADFPLNFAEFHLRKSSHNL
                     APEKLNVARYSFNKAWTCYNSYPYLKPRLRFRINHIRAYLLINEQKIPEALSIAEELI
                     ELTKNNNNKYIFIDAFETKALALEKSGRFDQAFHVMKKCFTNYRPEGDLGIVWYMHRR
                     MADLSLSSERYPACLNYCDSLMALILKSGHGNLTENVNWKDYSLHEVQVLIQVFKYRY
                     AAFSKWKKNRLSLDELKQLQLLFKKIHEATIYLHSVVYTDEKRLALEADHYPFYEKAI
                     EFNEILFDSTREAKYALNALTWSESSKNVLWSIRLLEQQKGNRYYHSNTINKIKSISR
                     KIRDTEYTLTEMNINPESSASKRKSELQSALASHSTEMELLKAQYSREVISFASAAIH
                     SEYPLAAIQNRLKMEHSVLLDYFYGDSLIALNLVSPDTAITHILPGNEELSNSILNYL
                     SAIKAPGIISSDSLRALSQNLLQILIKPVWEYLNAPNLVIIPDGPLNMIPFEMLLEGR
                     KTAQSVRYEYSSGDLLNENPVRPCTKYIGFAPEYSGLESIQYTHHDSLLLAGFYERNR
                     SSLGPLRFNREEVKESAEILKGKVFSGNQVSREVFNRASEQAGILHLALHALEDEEHP
                     EYSQFLLNSESGANAIDPLYSFQIKNLFLKADLAILSACNTGVGKYQRGNGVQSLAKA
                     FKQAGCNNILMSLWPVNDASTKEIVVGFIKNLKNGLGKADALAKAKQNYLAAAPEEMK
                     HPYYWAGLVLIGDNEPMFGSGVDWPVSSGWLIILLLVVSVFFVVWRRKWIN"
     gene            complement(14623..17643)
                     /locus_tag="PKM27_12740"
     CDS             complement(14623..17643)
                     /locus_tag="PKM27_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNR08178.1"
                     /translation="MRYLVFLVLLFIASVHSELADNQNNFQQAFPEYLKAAALEADVL
                     MDKADSLLFHHQNDSATFYFMLALPILEKLTAQTRDSMTWLKYATTLYNLGYTTQESF
                     EMDSSQHYLNRCLTATQERFGRIHLLTTLTLRKMEKNFMFKGEYNNSRNHIFEGFEIS
                     LKLKDQNPLFLGMAYQNMAYQNFLLNDREKMEIYYNKTEEIYESLYRIQDPSPNGSDT
                     AGWQWKFPNSIVAYFPRHYVDYHLRTAYNHLMVGRIRNAQYNFDQAYNCYLHHRQVQS
                     MMKFRIDNIRVHLLLYNGSFESALRLADSTIEFTRNDTDRYIFIDAYESKALTYETMG
                     RWEDAFQCMKKCFDIYRPKGDEGILLYMRSRLAQLALNARRFDFSRSIVDSMIGKFMP
                     EVRWDHPEPVEWTGREIYILKNMLPLLKHKFLIEMASLSNRFDPGEAMKTLKIFNAIH
                     EATVSLHERTYTDVNRLVHERDHHLLYEKAIELTQKLFGYSGDSRFLLKGLKWSEASK
                     SVILTREMVGQNQNRPYASSRTQRQLADLRRKVKTINYELAELHLRDSAQDSEMRLRL
                     QGRLAALYGEMEILKERNVKELESGQASQIRNEYPIQTVRQNLKEEQSALIDFFYGDS
                     LISIYLLTPDTLMNVQLVKSLSFDETVERFIDHIKIPKPNNLKEIDSLAQKLYQFLLE
                     PFAPWIAGLNLVIIPDGLLCFLPFEYLVTPIRGFQAVRYEYSSGLYVNKPGMAPHKRY
                     MGFAPEYSGRSTFNQSRGDSILLDDMYSTNRASLGFLKYNVPEVRECSKILEGQGFTG
                     KQINKSLFFSHARQAGILHLALHALANDQNPDYSQLLLNSEQVDMDFEPLYAYQIKEL
                     DLNAELAILSACNTGAGKYQHGNGVESLAKAFNQAGCNNILMSLWPVNDASTKEIVVG
                     FIKNLKNGLGKADALAKAKQNYLAAAPEEMKHPYYWAGLVLIGDNEPMFGLGPWESIG
                     PAQIGLLLGLFALMPLILFKYRTDRRSRIGRQRS"
     gene            complement(17859..18905)
                     /gene="ilvC"
                     /locus_tag="PKM27_12745"
     CDS             complement(17859..18905)
                     /gene="ilvC"
                     /locus_tag="PKM27_12745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNR08179.1"
                     /translation="MAKLNFGGVEENVVTRDEFSLAHAREVLKEETIAIIGYGVQGPG
                     QALNLRDNGFNVIVGQRSPSPTWDKAIRDGFEPGKTLLSIEEAVQRGTIVQYLLSDAA
                     QIAAWPAVKPLLTKGKALYFSHGFGITYKERTGIIPPPDVDVILVAPKGSGTSLRRMF
                     LQGRGLNSSYAIFQDATGKAKDRVVALGIGIGSGYLFETTFQKEVFSDLTGERGTLMG
                     AIQGIFEAQYTTLRRHGHSPSEAFNETVEELTQSLMPLVAENGMDWMYANCSTTAQRG
                     ALDWKNRFRDATAPVFEELYNSVARGDEAQRSIDSNSQPDYRAKLEVELAELRNSEMW
                     QAGKVVRSLRPEKQ"
     gene            complement(18951..19541)
                     /gene="ilvN"
                     /locus_tag="PKM27_12750"
     CDS             complement(18951..19541)
                     /gene="ilvN"
                     /locus_tag="PKM27_12750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNR08180.1"
                     /translation="MLQQEYTITVYTENQIGLLNRIAIIFSRRKINVESLNTSPSEID
                     SVHRFNIVITETEEVVRKLCRQIEKQVDVLKAYYNNNEEIVWQELALYKVSTDVIATE
                     VKVERLLSQYGARVVVIRKDYTVFSVTGHREETDRLIQVLEPYGLIEFVRSARVAIIK
                     SSEGFHRKLTQFEYREPASDVVENEFLNQQEKVFSM"
     gene            complement(19562..21319)
                     /gene="ilvB"
                     /locus_tag="PKM27_12755"
     CDS             complement(19562..21319)
                     /gene="ilvB"
                     /locus_tag="PKM27_12755"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNR08181.1"
                     /translation="MSSITQPLQETSPETQAEVMISGAEATMRCLIAEGVETIFGYPG
                     GAIMPIYDALFHHQHEVNHILVRHEQGAIHAAQGYARASGRTGVVFATSGPGATNLVT
                     GLADAMIDSTPLVCITGQVFAHLLGTDAFQETDVMNITTPITKWNYQVTEAHEIPVVL
                     AKAFYLAGSGRPGPVLIDITKNAQIQAFSFPAYEKCKHIRSYRPKPVIRPEYIKEAAK
                     LIDQAERPLVIFGQGVILGKAEKEFDTFLEKSGLPAAWTILGMSAIPTDHPNAVGMLG
                     MHGNYGPNLLTNECDVLIAVGMRFDDRVTGRLDKYAKQAKIIHLDIDPSEIDKNVKTT
                     VPVWGDCKETLPMLTDAIQRKSYPDWLKKFKECLEQEESKVIRKELNPSQEILTMGEV
                     INILNSLTGGNAILVTDVGQHQMVTCRYAKFQDSRSNITSGGLGTMGFALPAAIGAKY
                     GAPHREVVAIIGDGGFQMTMQELGTIMQFKPKIKIIILNNNFLGMVRQWQELFHENRY
                     SFVDISSPDYVQVAQGYGIRARSVSERKDLEPALKEMMGSEGTYLLEVQVGKENNVFP
                     MVPQGRGVAEVVLCKEDLL"
     gene            complement(21344..23020)
                     /gene="ilvD"
                     /locus_tag="PKM27_12760"
     CDS             complement(21344..23020)
                     /gene="ilvD"
                     /locus_tag="PKM27_12760"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNR08182.1"
                     /translation="MELNKYSKHVTQDPSQPAAQAMLHGIGLSDTDMKKPQIGIASTG
                     WEGNPCNMHLNGIAASIKGKINEEGMIGLIFHAIGVSDGISMGTDGMRYSLPSRDIIA
                     DSIETVVSAQWYDGLVTVVGCDKNMPGALMAMARLNRPSILVYGGTIAPGFYQGKKLD
                     VVSAFEALGQKVNGMITEEDLQEVIHRAIPGAGACGGMYTANTMSSAIEALGMALPGN
                     SSNPAVSEAKRLESQDIAKAIFNLIEKDIRPRDILTQEAFENAITLTMVLGGSTNAVL
                     HLIAIARAASVDLTIDDFQRISERTPFLADLKPSGKYVMQDLHEIGGTPAVLKMLLDA
                     GRLHGDCLTVTGKTLAENLASAPSLPAGQDIIRPWDSPIKSTGHIQILYGNLAPGGAV
                     AKITGKEGELFEGPAHVFDEEKDLNQAILDKKIKPGEVIVIRYCGPKGAPGMPEMLKP
                     TSALMGAGLGDKVALITDGRFSGGTHGFVIGHVTPEAQEGGAIALVKNGDIIRLDIKN
                     HSMDVLLSDEEISRRMRLWTKPPLKANSGILKKYAMTVSSASEGCVTDEF"
     gene            complement(23221..>24361)
                     /gene="ychF"
                     /locus_tag="PKM27_12765"
     CDS             complement(23221..>24361)
                     /gene="ychF"
                     /locus_tag="PKM27_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNR08183.1"
                     /translation="YNKKINMPLRGGIVGLPNVGKSTLFNALTSGKALAANYPFATKD
                     PNIGIIVVPDPRLDALAKLVNPQKTIPNTIEIVDIAGLIKGASQGEGLGNQFLANIRE
                     VEAIVHVVRCFEDDNVIHVDGSVDPVRDKEIIDMELLFKDMETMDKRIDRLKKQAKGA
                     NKEELRLLELAENLKKHLDAGKAARTFECEKNDRPLIDDFFLLTSKPVLYVCNVDEKS
                     IHSGNKHTRALEAVVAKENLTATGHAHSEIILISAEIEAQIAQLESMEERKEFLEAMG
                     LEEPGVNRVIRSCYKLLNLISYFTAGEKEVRAWTIHIGDLAPQAAGVIHSDFEKGFIR
                     AEVISYADYIKYGSEHAAREAGRLMIQGKDYVVQDGDVMHFRFNV"
BASE COUNT         5872 a         6201 c         6149 g         6139 t
ORIGIN      
        1 taagcgcagc ccatgaactc atcgagcggg gttttcaggt agaagtttat gaagctaacc
       61 cggtttattt cggcggcaag gcccgttcgg aagatcatac ggggataagt gccaatcccc
      121 tgcccgggga acacggattc cgttttttcc ccggtttcta caggcatatc acccatacca
      181 tgaaaagaat ccctttcggc gccgggacct gctatgacaa cctggtgacc acggaaagga
      241 tcctcattgc aagaaacggc caggaaccca ttaaaatcca ggcggagttt ccattcacgg
      301 cggcaggatt taacctggtg ctcagcaccc tgtccggcgc ggacacgggt ttgaccggcc
      361 ctgaaaaaaa actttttaaa aacaaactct ttcaattgct taccacaagc aaggagagat
      421 tcgaaagcga atacgaacag caggcctggt ggtatttcgt agaagcgcat cagaacagcc
      481 ccaattacca gaatctcctg gcggacgggc tcacccgctc cctggtggcc gcccaggcca
      541 aaaaaatgtc gacccgggta ggaggaggta ttctcctgca attaatctat gatatcgtag
      601 atccgggcat ccccgcggac cgggtgttaa acggccccac caaccagcag tggatacaac
      661 cctggatttc ctaccttcag gccaaaggag tacaattttt tcttaaccat accgctagtg
      721 cgctcaacgt ggatgcggtt ggaaggcttc aatccgtaag gctggataac cccgctaccg
      781 gaatcccggt atatgcaacg ggcgattatt ttatcctggc cgttcccgtc gaaaaagcca
      841 aagacctcct ggaccatccg gtgatcacca accgggaccc catcatgagt ttcccaaccg
      901 aattgtccaa aagcgtgaga tggatgaatg ggatccagtt ttatctcagc actgaaattc
      961 ccctcaaccc gggacatatc attcatatcc acagcccctg ggcagtgacc agcatttcac
     1021 aggttcaata ctggtccgga tacgacatcg attcacagtc cggcggcctt gtgaaagggg
     1081 ttttgtccgt cgatgtgtcc gattggcagg acggaattta taatggcaaa cgggcaaaag
     1141 acacctccga agccgaattg atccaatcca cttatgagca gatccagcaa agcctgatcg
     1201 ttggcggtgt gcccctatta cccgcaaacc gggccgatgt agtggtcgga taccatattg
     1261 cctccagcat tcgccgcacc gggcccaccc aagcggtcag cgatgaaccc ttgctggtaa
     1321 ataccgtaaa ttcatgggat cttatgccca aatcctttac cggaataccc aatttgttcc
     1381 tggccggaga ctatgtcagg actttcaccg gcctggctac catggaagcg gccaatgaat
     1441 cggcgcgacg cgcggtgaac tgcatcctct ctgcttcagg cacgggcaaa ccttatgcaa
     1501 aagtctggcg attgcgcgag tgggccatgg tattgcctta caaatggtgg gaccggcgga
     1561 gatattccaa aggattgccc tattcctccg gctttccctg gtggttgcac gtgattgcct
     1621 ttatctggag cataatctgc ctggtggcct gggcgattgg tttacccttc cgctggcttt
     1681 tcggattcat ttgaccgcgt acccccatgc tgaataaact taaagcctgg ctggattcgc
     1741 tgatcattcg gcatttgccg gacagcggcc atcacaaaca ggcgaggatg attgtacggg
     1801 gcagcctgct gacggtattg ttcatggcct attatgccct catcagcaaa gtaatcagct
     1861 ataaagaggg agtcctgatc caaagcattc tgaccctggg tttcgccctg attcccctgg
     1921 cagtcctccg ccgggcaaac ctgaatctca tcgggaacct cttcgttttt tcagggtgta
     1981 ccggcatcgt gatgttgtcc ttgcataccg ggggcctgga atcgcctgtt cttccgtgga
     2041 tcggactcgt acccatcgtc gccttgcttt ttaccaaccg caattgggcc tgggcctggt
     2101 ttgtacttac ccttgctgtg gtggccttat ttatttattt gaaataccaa ggaatgatac
     2161 cctcgccgag atttgacacc agttataaag tcttcctggc cgccaattgt ttgatcggac
     2221 tggtgctcac ccttttcctt ctcaatacca tttttgtaaa ctccatccgg gagatcaatc
     2281 aacagctgga gtccaaaaac caggagatag aagaaaaaaa caaacttctt gaacaacagg
     2341 ccggccagat caccgcgcag agggaccaga tcatcctgga gaaacaaaaa tcggacaagc
     2401 tcctgctgaa tatcctgccc gcggatatcg ccgaagaact caaacaaaac ggaagctgtg
     2461 aagccaggca atacgaaaac gtgagcattc tttttacgga ttttgtcaat tttaccaatt
     2521 acagtgaaaa aatgagcccg caatccatca tcgccgaatt gcaccgttat tttaccgctt
     2581 ttgatgccat cattgaaaaa tacggccttg aaaaaataaa aaccatcggc gatgcgtata
     2641 tggcagtcag cggtttgccg gaaaggaaca gcaaccatgc ggtgctggcc atcctagccg
     2701 cgaaggaaat ccgggaattc gtgcaggctt ccatgaaccc ggagggccgg tcatccgaaa
     2761 actccgggga gggaaagcaa ggattccagg cgaggatcgg gattcattcc gggcccgtag
     2821 tggcaggaat tgtgggcagc aaaaaattcg tatttgacat ttggggggac gccgtcaata
     2881 cggcagcccg gatcgaacaa aacggctttc cccagaagat cagcatttcc gggcaaacct
     2941 atgagttggt caaggatcat tttccctgtg aataccgggg gaaggttaaa gccaaaaaca
     3001 agggcgaact ggacatgtat tttgtaaact gagccggctt taccgggtga cggtttgcac
     3061 ccgggccagt ccttcgtggt aatcgcggac atccggaaac aagccattgt gaatcgattt
     3121 gccctcccgg ttgatgaatc cgatcccttc gcccaggctg tatcttgcca cgccttcgtg
     3181 aaaatcccag gccagcagga agatattggg gatggcatga cggccattct cgtccatata
     3241 tccaaacaga tcatccttgc tgaaaacggc cagtccttcg tgaaacgcgt tgatctgatt
     3301 atattgaaat gggctcaaca acaggccatc cggtgacatc agggcgaacg aatcattttt
     3361 ctggaccgcc cagcgccctt cacccagagg ataaatatag ttccagtcca ggcccggccg
     3421 cgggaggcct tgccaggggc ggccctgaag gtcaatgagc atccatttgc cgtctttcgc
     3481 aacggccgcg gatccctcct ggaaggcggt cgcctcatca aactgggccg gtatcaccag
     3541 ttcatgaagg gtatccacat atccccattt gcctttaacc tggacgggta tgcggttttc
     3601 cgaaacctgg ccgatggcat cgtatttcgg cgggatgatc attttcccgc cccggttaag
     3661 gataccccac cagcccccgt tggagatacg ggcataccct tgattgaaac caaaagcctg
     3721 gtcccaggaa tcttcggaga tcagccgtcc ttccaggtcg ataaactggt accggttgtc
     3781 ttttttgacc cttgccacac cttcatggaa atcttcggcg tcctcccata gggtgtccag
     3841 gaccagtatt ccattggtat ccaggaatcc gtatttccca ttcagtattt taatccgtcc
     3901 gcgattttga ctgaaggacc aggctccccg gtactgggcc ttcaccagcc attctccgga
     3961 ccggtcgata taaccccaca atcctttgtc cgaagcggcg gacaagccgt ccgaaaaatt
     4021 cctgcaatcg tcaaattggg ttttgatgac caggtcgcca tccagatcaa tatagcccca
     4081 tttgatgccc ggttcgatat aatcggcgct gacgatatcg ctttcagaca ggggttcgat
     4141 cagggttccg cgatcggatt cgggtttgca cgcggattga aaaaatagaa gaaaaaggat
     4201 aaaatagatg aattttggta caatatccaa ttgggtcagg cgaaatggca tatggcaaaa
     4261 ttattgttat gctgcttatt atttccggtt tttaatcccc tggccgccca gaaaccgacc
     4321 atcctggaac ccgccaattg cgccgttttg atcccgggta ccttccagta ttcggcggat
     4381 acgatcccga ccgaagtggg cgagatcatc tatcatacct ggcactccag ggacagtacg
     4441 gtccagtacc tggtttcttt ttgtaactat ccagaaggaa cagtgcattc cgactccacc
     4501 gaattgctgg aagaattttt tacagccacc atctcggcga ctgtggaaaa aacccgcgga
     4561 atcaagaggt atgagtccgg tatgctgcaa tccggtttcc ctggccggtt ttggcgaatc
     4621 gatataggaa cccgttacgt caaaacccgg gcttttgttg cagggacccg tttttacagc
     4681 ctgcaggtca ttggcccgct tggcgcggaa caggacaaat ccgaatccgc tttttttgat
     4741 tcttttcgat tcctggatct tttgagagca aggaagtaag gcctgttcct ttcatttaca
     4801 ctaattttgg ctttccggat tccggctaaa ccagaccatc agatatgatc atcgcagtat
     4861 ttccagggtc ttttgacccc atcaccatcg gccatgtgga tgtggtcaat aaagccctgc
     4921 cgctgtttga caaggtcatt gttgccattg gcaccaacac tcaaaaaaaa tccctgttca
     4981 gcctggagca gagaaaacaa tggatcatgg atgttttcca gggcgtggat aaagtggagg
     5041 tcgatcattt tgacggattg accgccgtat atgccaaccg gatccgggcg aattacctga
     5101 tccggggcct gcggaacggg agtgattttg attatgaaaa gacgatcgcc cacgtcaact
     5161 cgaccattgg gcatggcctc gaaacggttt ttttcatcag cagccccgag gtggcccata
     5221 tcagcagtac catcgtaagg gagatcattg ggggcggggg ggatgccacc atgtttattc
     5281 ccagggaaat ccatttaaaa ccactcactt tttgaaaccg ataaccatat gtccggaaat
     5341 tttaaacttc cccctgtaaa aaatgaaccc gtgccggagt acctgcctgg aagcgctgaa
     5401 aaactccagc tcaaactggc cctggcgaaa gccaaatcaa gcactcctga aataaaaatg
     5461 accattggag ggaaaaagat atccaccggg ccttccatcc ccgtacgtgc accccatgac
     5521 caccagcaga tcctggccta tcacagcaaa gggggaaagg aacaggtgaa ggcagccatt
     5581 caggcctccc tgaatgcaaa agcggcctgg cagcataccc cttgggaaga acgggcggct
     5641 gtattcctga aagcggccga tctcctcagc ggcccatacc gggcgcgcat gaatgccgct
     5701 accatgcttg ggcagtccaa aaacgtattt cagtctgaaa tcgacgccgt agctgaactg
     5761 gccgattttt tccggttcaa cgtgaagtat atgacggaga tctatgccac ccagcccgcg
     5821 agccctcccg gtacctggaa ccgtctggaa taccggccgc tcgaaggatt tgttctggcc
     5881 atcacccctt tcaattttac atccattgca ggtaacctgc ccggggcgcc cgctttgttg
     5941 ggcaataccg tggtgtggaa accggcggaa acccagatct actccgccgc aatcattatg
     6001 gagatcctcg aaaaggccgg gctgcctgac ggagtcatca atatgatcac catcgacggg
     6061 ccaagcctgg gtgaggtggc tttcagtcat cccgatttcg caggactgca ttttacgggc
     6121 agtacaggcg tttttaatca tctatggcag accattggca accaattggg ccggtacaag
     6181 tcttatccca ggctggttgg agaaaccggg ggtaaggatt ttgtgctcat gcacgcctct
     6241 tccgacccaa aagccgtggc tacggccttg agccggggcg cttttgagta ccaggggcag
     6301 aaatgttcgg cggcatcccg agcctatatc cccaaatcgc aatggaaaaa ggtgcaggag
     6361 tacctcctgg acgacctggc ttccatgacc ataggcgagc cggaaaattt cagtcatttt
     6421 atcaacgccg tgatcgacga aaaggcattt gataaaatca gttcctacat tacgggagcc
     6481 cgtaagtcaa aggacgcttc ccttttggcg ggaggccggc cggacaaatc caaaggctat
     6541 tttatcgaac ctacggtcat cctggcgaac cggccggatt ataaaaccat ggtggaagaa
     6601 attttcggac ccgtcctgag catttatgtc tatgaggaca aatcgtttga ggatacgctg
     6661 aaatggatag actccacttc tccctatgcg ctcaccgggg cggtctttgc gactgaccgg
     6721 caagtgatcc agcagaccag caggcagctg atgcaggccg ctggtaattt ttatatcaat
     6781 gacaagccta cgggagctgt ggtgaatcaa cagcccttcg gaggcgcgag aggaagcggg
     6841 accaacgaca aagcgggttc gatactgaat ctttaccgtt ggctttcccc gcgcaccatc
     6901 aaagaaaact ttaatccgcc ccgggactac cgttatccct ttatgtccga ggaataaaaa
     6961 ccagatcatg aaaatataca cccgcaccgg ggatgagggc atgacttccc tttttggtgg
     7021 gacccggctg cccaaacatc atgtgcggat tgaagcctat ggcagcctgg atgaagtaaa
     7081 tgcctggata ggcctcgtcc gggaccaggt ttcagatgaa aatacagcgg ccgtccttaa
     7141 aaagatacag gaccatttgt ttgccatagg ctcccacctt gcagcttcac cggataaaaa
     7201 actccccctg ccggaagtca ccggggagga tatcaaactt cttgaggatg aaatggacca
     7261 tatgaccacc caggtcagcc cattgaaaca ttttatactt cccggcgggc ataagcgggt
     7321 cagcgaaatt catttggccc ggaccgtttg caggagggcg gagcggaggg tgatggcctt
     7381 gcaccaggaa tccccggtgg agccggtgat cctggtcttt ctaaaccgct tgtcggatta
     7441 tcttttcatc ctgggccgct ggaccgccca tcaactcgga attgaagaaa taaaatggaa
     7501 tccccgcaag gaatgaacct ggaccttgct gcgatcgatc aaaaggattt ggagggcctg
     7561 aaagcttttc aaccggcgga ctggccggat atcaccaaag cctatgcttt ttatattccc
     7621 agcccctatt gccagccatt aaaattgatg gaaaacgggg aaatccgtgc cataggctgt
     7681 gccatcaggc atgcacagac ggcatggctg gctcagatca ttgtagccga accatacaga
     7741 aaccgcggct taggaagtaa aatgacccag tgcctgatcg acctggtgaa acccgaagta
     7801 aggtccattt ccctcgtagc caccgacctg ggcgcccctg tttataccaa actgggattc
     7861 cggccggtgg ccgaatattc tttcttcagg agggccgaac ccctgccccc caatccggaa
     7921 tccggagtag cagatctcaa ttcttccatc ctgggccagg tcctggaact ggatctggaa
     7981 gcctcgggtg aagaccggtc cttgctcatc cgggatcatc ttgaaaatgg gaaggtaatt
     8041 tcttctaatt ctgtgcttta cgcatattat cttccaacat taggtgaggg gttgatcatc
     8101 agccgggatg agaaagccgg ccaggaactg atgcggctga aactatctgc ccggctacgc
     8161 catgtaattc cggaaggaaa tgtaacggcc aaaaattttt tacttgaaaa ctgctttgag
     8221 ccttacagga aagcgttgcg aatggtattg ggtgatccgg tacattttaa accggaatgg
     8281 atttattcaa ggatcggagg taacctcggc taagcacctc taacctctaa cctctaacct
     8341 ctgacctata tatctagtaa cccaaacata actcccgaac aaaaccccac taaacaataa
     8401 gtcagccgca aacgtattcc agaaaaaggg aagggcaaat tcataacagg caaggagtcc
     8461 ggccggattc tgaggccagg ccgggctatt gatccagacg ccgaaattgg tgaccatgaa
     8521 gaacaaaagg gaagcaagga tagatgaaag caacacattt tttacattga tcttccttat
     8581 gccccattga cccaaggcga cgatgaggac aaatccggta taaacccagg cattgcccat
     8641 ccattgccag ccggtgtagt gctcgggaaa taggttccgg tacatcccgt tgttcaggta
     8701 aaggtcggag agccataaaa tcaacaaagg aataataaag gacatccacc tggggcggat
     8761 cagggccccg gcgaacaagg cggtggctcc gaccgccgta aaattgggag catgcgggat
     8821 cagcctgctc aggataccgg caaggaccag tcccaggatt aaattagagg agataaattt
     8881 tttcacgccg gttcaaatta ctggtttaaa taatgcgtaa aattacttta aaataggcaa
     8941 tctgaacaac aatatccggg ccacgcccga aaaagcggca ctggctgatt ttgaggccat
     9001 cctggctctt tataaggagg tcaccgcctt cctgatgaag cagggcatct atcagtggga
     9061 cgacggttat cccgatgctt ccaccctctg gcaaaacctg gaggaagggg atacctggat
     9121 catgcgggat ggagatgttt tgacggccag cgtgaccctg gaccggaacc aggaccccca
     9181 gtacgaaaag atcggctggg cctatccctc ggatcgattc ctggtggttc accggctttg
     9241 cacgcatccc ggctaccagg gcaggggatt ggccagacaa ttggcctatt ttgctgaaca
     9301 gcaggcccag gacctgcaac tggacgtgat ccggctggat gcctatctcg gtaatccggt
     9361 ttctcaaaaa ttgtaccgct cgctgggtta tcatgaagcc tacggatatt gttattatcc
     9421 tccccgggac atcatgtgca attgttttga gaaatgggtg gggtcatgag gcatttgaac
     9481 ggcagctgct tttaatctat ttttagacca tgaaacggct ttcgatactg atggtcctct
     9541 gcctgggagg aggattgatc caggcccagc ccaaccgctg gcagcaacag gtctcttata
     9601 aaatggatat cgattttgac gtccaatccc accggttcac cggccagcaa accctggttt
     9661 ataccaacaa cagtccggat gtattggacc gggtctttta tcatctctat tacaatgctt
     9721 tccagccggg aagcgtcatg gatgtccgct cccggaacct gcccgatccg gatggcaggg
     9781 tgagggaccg gatatccaag ctcaccgaat cagaaattgg ttatcacgaa attctaagcc
     9841 tcacccagga tgggatacct gtgaaatacc atattgaagg caccatcctt gaggtcaaac
     9901 tggccaaacc cttgaaatcc aaagggaaaa ccaccctggc aatgaaattc cgttcccagg
     9961 tgccggtcca gatccgcagg accggacgca acaaccggga aggcatagcc tattccatga
    10021 gccagtggta tcccaaactc tgcgaatacg actaccaggg ttggcatgcg aatccctatg
    10081 tcgccaggga gttttacggg gtctggggcg attttgacgt aaagatcagg atcgataaaa
    10141 agtacctggt agcagcgggt ggttatatcc aaaatcccca ggaagtgggt tatggttatg
    10201 aaaaaccggg aagccaggta aaaccgcctt ccggcgaaaa actgacctgg catttcaaag
    10261 cgccccgggt gcatgatttt ctgtgggctg cggacccgga atacaaacac accaggctgg
    10321 tcaatgacga cggcattgac atgcatttct ttttccagga aaacgacaaa acccgggata
    10381 cctggtccag gctgcccgca gtgatgaacc aggtattcaa gatcgccaat aaaagatttg
    10441 gaaaataccc ttattcctat tacgctttta tccagggcgg ggatggggga atggaatatc
    10501 ccatggcgac ccttatcctg ggagaaggat cgatggatgg gctcatcggg gtatccacgc
    10561 atgaactcct ccatagctgg taccagatgg ttctggggtc caacgaaagc ctctatgcct
    10621 ggatggatga aggatttacc agttacgcgc aagacatcat ccttgccgaa atcgaaaaac
    10681 cgggcagcgc ggaccaatac gggactggcg cctgcgcggg ggcgtaccag ggttatttca
    10741 acctggcgaa aagcggccgg gaagagccgc tcagcaccca ttccgaccat tatgaatcca
    10801 attttgccta tggggtggcc gcctattcca aaggggaggt gttcctgcat cagcttgaat
    10861 acatcatcgg gaagcaggcc ttccaggccg gtttgctgga ttatttcaat acctggaaat
    10921 tcaaacaccc gaacgtcaat gacctggtcc gggtttttga aaaacggagc ggactggagt
    10981 tggattggta caaagaatat tttgtgaaca gcaccaagca gatcgattat gccgtcgatt
    11041 cggtctacga agaaaacggc caggcccgga tcgtgatccg caagaaaaaa ccgttcatca
    11101 tgcccctgga tatcctggta tctcttaccg acggcagcca aaacctggag tatatccccc
    11161 tggacctgat gcgcggggag aaaatccagg aatggccgga taaaaagctc aatctccagc
    11221 ccgactggac ctggacctat cccagttata ccctgaaact gaaggtcccc tttagccagg
    11281 ttgccggcat tgagatcgat ccttcaaagc ggatggcgga tacggacagg gagaataacc
    11341 ggtgggtgag gaagtgattc gggtagcgag atacgagtag tgagaagcca gatacgagta
    11401 gtgagaagcc agatacgaga aacgagaagc gagccgatca actggtaacc tgataaactg
    11461 gttaactgtt ttaattgatc cactttctcc gccaaaccac gaagaagacg ctgacaacca
    11521 aaagtaaaat aataagccac cccgatgaaa ccggccagtc tacacctgat ccaaacatcg
    11581 gctcattgtc cccgatcaat accagccctg cccaataata gggatgtttc atttcttccg
    11641 gggctgcggc gagatagttt tgttttgctt tggccagggc gtccgctttt ccgaggccat
    11701 ttttcagatt tttgatgaaa ccgactacaa tttctttggt gctggcgtca ttgaccggcc
    11761 agagagacat caggatgtta ttgcatccgg cttgtttaaa ggctttggcc agggattgca
    11821 ctccgttgcc ccgctgatat tttcccactc cggtgttgca ggcactcagt atggccaaat
    11881 cggctttgag gaataaattt tttatctgaa atgaatacag gggatcaatg gcattggcgc
    11941 ctgattcgga attcagcaga aattgggaat attccggatg ttcttcatct tccaatgcat
    12001 gcaaggccag atgaaggatg cctgcctgct ccgatgcacg gttaaaaact tcccgactta
    12061 cctgattccc cgaaaatact tttcctttga ggatttctgc tgattctttg acttcctccc
    12121 ggttgaatcg caaaggaccc aggcttgaac ggttgcgctc ataaaatcca gcgagtaaga
    12181 gggaatcatg atgggtatat tgaatggatt ccaaaccgga gtattccggt gcaaatccga
    12241 tatactttgt gcatgggcgg accggatttt catttagtaa gtcgccgctt gaatattcat
    12301 aacgtacgga ctgggccgtc ttccgtccct ccagcagcat ttcaaacggg atcatattca
    12361 agggaccatc cgggataatt acgaggtttg gggcattcaa gtattcccaa acgggtttta
    12421 tcagtatctg caaaaggttc tgggacaagg ccctgaggct gtcggaggaa atgattccgg
    12481 gggctttgat ggcggacagg tagtttaaaa tggaatttga aagctcctca ttcccgggca
    12541 ggatatgagt gatggcagtg tccggactga ccagattcag cgcgatcagg ctgtctccat
    12601 aaaaataatc aagcaaaacc gagtgttcca ttttcaaccg gttctggatg gcggccagag
    12661 ggtattcact gtggatggcc gcggacgcga aggagatgac ttccctggaa tattgggctt
    12721 tcagcagttc catttcagtg gaatgggatg ctaaggcgct ttgcagttct gattttcgtt
    12781 tggatgcgga tgattcagga ttgatgttca tttcggtcag ggtatattcc gtatccctga
    12841 ttttccggga aatggacttt attttgttta tggtattgga atggtaatac cggtttccct
    12901 tctgttgttc cagtaatctt attgaccaca acacattttt gcttgactcg gaccaggtga
    12961 gcgcatttaa agcatatttt gcttcccggg tggagtcaaa taaaatttca ttgaattcaa
    13021 tggctttctc atagaatggg taatggtctg cttccagtgc caatcgtttt tcatccgtat
    13081 aaaccacgga atggaggtag atcgtcgctt catgaatttt cttaaacaac agttgcaatt
    13141 gtttaagttc atcgaggctt aagcggttct ttttccattt actaaatgcc gcgtacctat
    13201 acttaaacac ttgtatcaat acctggacct catggaggct gtaatccttc cagtttacat
    13261 tttcagtaag gttcccgtga ccagacttca ggatcagggc catcaggctg tcacaataat
    13321 tcaggcaggc cggatatctt tctgaactta atgacagatc ggccattctc cggtgcatgt
    13381 accagacaat gccaagatct ccttcgggcc ggtagtttgt aaaacatttt ttcatgacat
    13441 gaaacgcctg gtcaaaccgg cctgactttt ccaaggccag ggccttggtt tcaaaagcat
    13501 caatgaagat gtatttatta ttgttgtttt ttgtcaactc gatcagttcc tctgcaatgc
    13561 tcagagcctc cgggattttt tgctcgttga tcaaaagata tgccctgatg tggttaatgc
    13621 ggaaccggag cctgggcttt aaatagggat atgaattata gcaggtccac gccttattaa
    13681 agctgtacct ggcaacattc agcttttcgg gtgcgagatt atgtgagctt tttcgaagat
    13741 gaaattccgc aaagtttaaa gggaaatcag ccaataccga attgggctgc gggccatcgg
    13801 catggaccgg ttttgtagga aagggatagg attcggatcc ggcggggcct cccccgtagc
    13861 gggctagggc tgaatcataa atttgttccg ttttattaaa atagatttct atttttttct
    13921 gatcattcaa taaaaaatac tgatagccca tttcatgata tgcctgtccc agcaggaaat
    13981 catacttccc ggaaattctt tggcagatgt taaatccctt taaagtgcaa gaccgttcgg
    14041 catttccatc ccccatcgcg aggtaggatc tggccatctt gaaataagtc catgcggcgg
    14101 tggggtgatc ctcgccaaac ctctccagga ccactttcaa actctgttga agatattcca
    14161 gccccttctt catgtccctg ttcacctgat ggcagaatcc tatggaaaac agcgcattcg
    14221 cgtagcctgc ccagatcagg gaatcccgtg tggagtcaac gattctttga tactgattcg
    14281 cagatttttc aaaataaata atcgccgaat catattgaag attgaaaagg gccgaatccg
    14341 catgttgaaa cagggtggag gccctgatcg aatcttctgc aatggaaaaa tcaggcccgg
    14401 gtttgatttc agctgttttc tttgccggaa cacgggaatg aaccgttgcg agttgaaaga
    14461 ataacaataa aaagaaatat ctcataccgg tgatcaaaga cgattcgtgg ttgcaaaccc
    14521 tgtccacaaa aggatgtgac aaagtccact ttaatataga ttgaattttg gaatttttcc
    14581 aaccattgga ttttcaggat ttcaaaccct ggaatcccat agtcatgacc gctgtctgcc
    14641 tatccgtgat cggcggtcag ttctgtactt aaataaaatc aggggcatta aagcaaacaa
    14701 gcccagcaag agcccaatct gggcaggacc aatggattcc catggtccca gaccaaacat
    14761 cggctcattg tccccgatca ataccagccc tgcccaataa taaggatgtt tcatttcttc
    14821 cggggctgcg gcgagatagt tttgttttgc tttggccagg gcgtccgctt ttccgaggcc
    14881 atttttcaga tttttgatga aaccgactac aatttctttg gtgctggcgt cattgaccgg
    14941 ccagagagac atcaggatgt tattgcatcc ggcttgatta aaggccttgg cgagggattc
    15001 cactccgttg ccgtgctgat atttgccggc gccggtattg caggcgctaa ggatggcgag
    15061 ctcggcgttg aggtccagtt ctttgatttg gtaggcataa aggggttcga aatccatatc
    15121 cacctgctct gaatttagca gcaactgcga ataatccgga ttttggtcat tggccagggc
    15181 atggagcgcg agatggagga tgcctgcctg ccgggcatga ctgaaaaaga gcgatttatt
    15241 tatttgctta cctgtaaagc cttgtccctc caatattttt gaacactccc gcacctcagg
    15301 gacattatat ttcaaaaatc ccaggctggc ccggtttgtg gagtacatat catccagcag
    15361 gatggaatcc ccacggctct gattgaaagt ggacctgccg gaatattcgg gggcaaatcc
    15421 catataccgt ttatgggggg ccattccggg tttattgaca taaagtccgc ttgaatattc
    15481 atatcggaca gcttgaaatc cacggatagg ggttaccaga tattcaaaag gaaggaaaca
    15541 aagcaggcca tcaggtataa tcacgaggtt cagacctgca atccacggcg caaaaggttc
    15601 caaaaggaat tgatatagtt tttgtgcaag ggaatcgatt tctttcaggt tgttgggttt
    15661 cgggatcttt atatggtcaa taaaccgttc aaccgtttca tcgaaggaaa ggctcttaac
    15721 caactgcacg ttcatcaggg tatccggggt caggaggtat atggagatca gactgtcgcc
    15781 ataaaaaaaa tcaatcaggg ccgattgctc ctccttcagg ttttggcgga ccgtttgaat
    15841 cggatattca tttcttattt ggctagcctg tccggattcc aactccttaa cattccgctc
    15901 cttcagtatt tccatttctc catataaggc agccaggcgg ccctgcaatc ggaggcgcat
    15961 ttcagagtcc tgggcactgt cccttaaatg taattctgcc agttcatagt taatggtttt
    16021 aaccttgcgg cggaggtctg ccaattgcct ttgtgtacgc gatgatgcat aaggccgatt
    16081 ttgattctgt ccaaccatct cccgggtgag gatgacgctt ttgctggcct ctgaccattt
    16141 taaccctttc agtaaaaatc gtgaatcccc tgaatagccg aataattttt gggtcagctc
    16201 tatggctttt tcgtatagaa ggtggtgatc gcgctcgtgc accaggcggt tgacatcggt
    16261 ataggttctt tcgtgcaaag agacggtagc ttcatggatg gcattgaata tttttaatgt
    16321 tttcatcgcc tcccccggat caaaccggtt tgacagggaa gccatctcaa ttaaaaattt
    16381 atgcttcaat aaagggagca tgtttttgag tatgtatatt tcccgcccgg tccactccac
    16441 aggttccgga tggtcccagc ggacctcagg cataaacttg ccgatcatac tgtccacgat
    16501 ggaccggctg aagtcaaatc tcctggcatt taaggcaagc tgagccaacc tgctgcgcat
    16561 atataacagg atgccttcgt ctcctttggg ccggtagata tcgaaacatt ttttcatgca
    16621 ctggaacgcg tcctcccaac gacccatggt ttcataagtc agggctttgg attcgtaggc
    16681 atcaataaaa atatacctgt cggtgtcgtt tcgggtaaat tcaatggttg aatcggccaa
    16741 tctcaaggcc gattcaaatg atccgttata aaggaggaga tggactcgga tattgtctat
    16801 cctgaacttc atcatggatt gaacctgccg gtgatggagg taacagttgt aggcctgatc
    16861 gaagttgtat tgggcattgc ggatcctgcc caccatcagg tggttgtatg cggtccgcag
    16921 gtgataatcg acgtaatgcc tcgggaaata ggccactatg gaattgggaa atttccattg
    16981 ccatccggca gtatcggaac cgtttggtga gggatcctga atccggtaca gcgattcgta
    17041 aatttcttcc gtcttattgt aataaatttc cattttctcc cggtcgttca ggaggaaatt
    17101 ctgatacgcc atgttctgat aagccatccc gaggaaaaga ggattctgat cttttaattt
    17161 cagactgatc tcaaatccct caaatatgtg atttctcgaa ttgttgtatt cacctttaaa
    17221 cataaagttt ttttccatct tcctgagcgt cagggtagtc aataagtgaa ttcttccaaa
    17281 ccgttcctgc gttgcggtca ggcaccgatt caggtaatgc tggctggaat ccatttcaaa
    17341 tgattcctgg gtagtgtaac ccaaattgta cagggtcgtg gcatatttca gccaggtcat
    17401 cgaatcccgg gtctgagcgg ttaatttttc aagtatcggc aaggccagca tgaaataaaa
    17461 cgtggctgaa tcattttggt ggtgaaacaa cagggaatcg gccttgtcca tgagaacatc
    17521 cgcttccagg gcagctgctt ttagatattc cggaaaagct tgttgaaagt tgttttgatt
    17581 atccgcgagc tcagaatgga ctgaggcgat aaaaagcaat accagaaaaa caagatatct
    17641 cattgcagtg attcagggct aagccgcaga cgcatccgct gtctccaccg gctaggtcaa
    17701 tccttgcttt aatttaattt gaatttttta tttaccccga acaaagttga atttcaggca
    17761 tacaatcctt gatcctgttc ctgactactg atccctaaca aaaaggcccc agatcacccc
    17821 ggagcctttt ccaatcatat ctatctaaaa agaatctttt attgtttttc cggcctcagg
    17881 ctgcgcacga cctttcctgc ctgccacatc tcggaatttc tgagttcggc cagttccact
    17941 tccaacttcg cccggtaatc cggctgactg ttgctgtcta tagaacgctg ggcctcgtct
    18001 ccgcgcgcca ccgaattgta taattcttcg aaaaccgggg ccgtcgcgtc ccggaagcgg
    18061 tttttccagt ccagggcgcc ccgctgggcg gtcgtgctgc aattggcata catccagtcc
    18121 atcccgtttt cggcaaccag tggcatgagg ctttgggtca gctcttcgac ggtctcgtta
    18181 aaggcctcgg aaggggagtg gccatgcctt ctgagggtgg tgtactgggc ttcgaatatt
    18241 ccctggatgg cgcccatcag ggtgccgcgt tcgccggtga ggtcgctgaa tacttccttt
    18301 tggaaggtgg tttcaaaaag atatcccgag ccgatgccga tccctagggc gaccacccgg
    18361 tccttggcct tgcccgtggc gtcctggaaa atggcataac tcgaattgag gcctcttccc
    18421 tgcaggaaca tgcggcggag gctggtgcct gaaccttttg gcgccaccag gatcacatcc
    18481 acatccggcg ggggaatgat gccggtgcgt tccttatacg tgatgccgaa tccatgtgaa
    18541 aaatagaggg ccttgccttt ggtcagcagg ggcttcaccg caggccaggc cgcgatctgg
    18601 gcggcgtcgg aaagcagata ttgaacgatc gtgcctcttt gaacggcctc ttcaatggaa
    18661 agaagggttt tgcccggttc aaatccgtcc cggatcgcct tgtcccaggt gggagagggg
    18721 ctgcgctgcc ctacaatcac gttaaaccca ttgtccctca ggttcagggc ctggccgggg
    18781 ccctggaccc cgtatccgat gatcgcgatc gtctcctctt tcaacacttc acgggcgtgg
    18841 gccagtgaaa attcatcgcg ggtaaccaca ttctcttcta ctccgccaaa gtttagtttt
    18901 gccatttaca gtttatgatt taaaaattag aaaatgctgc ctggtccggc ttacatgctg
    18961 aaaacctttt cctgctggtt gaggaattcg ttttccacga catcggatgc cggttcccgg
    19021 tattcaaact gggtgagctt ccggtggaag ccttcgctgc tttttatgat cgcgacccgc
    19081 gcactgcgta caaactcgat cagtccataa ggctcgagca cctggatcag ccggtcggtt
    19141 tcttcccggt gaccggttac gctgaatacg gtataatctt tccggatgac gaccacccgg
    19201 gccccatact ggctcaacag acgctccacc ttgacctcgg tggcgatgac atccgtggac
    19261 actttgtaga gggccagttc ctgccacacg atttcttcat tgttgttgta ataggccttc
    19321 agcacatcca cctgcttttc gatttgcctg cacagtttgc gcaccacctc ctccgtttcg
    19381 gtaatcacga tgttgaaccg gtggacggag tcgatttcgc tgggagaggt gttcaggctt
    19441 tccacattga tttttcgcct ggagaatatg atggcgatcc ggttgagcag gccaatctgg
    19501 ttttccgtgt agacggtgat cgtatattcc tgttggagca taaatttaga tttgacgtgt
    19561 tttataaaag gtcttccttg cataagacga cttcggccac ccctcttccc tggggcacca
    19621 tgggaaatac attgttttct tttcctacct ggacttccag gagataagtc ccttccgacc
    19681 ccatcatttc ttttaaggcc ggttcgaggt cctttcgttc cgatacgctt ctggcccgga
    19741 tgccgtagcc ctgggccacc tggacataat ccgggctgct gatatccaca aaactgtagc
    19801 ggttttcatg aaacaattcc tgccactggc ggaccatacc caggaaatta ttgttgagaa
    19861 tgatgatttt tattttgggt ttaaattgca tgatggtgcc cagttcctgc atggtcatct
    19921 gaaatcctcc gtccccgatg atggccacga cttcccggtg tggggccccg tatttggccc
    19981 cgatggcggc cggcaaggca aaacccatgg tgcccaggcc cccgctggtg atgttgctgc
    20041 ggctgtcctg aaatttggca tagcggcagg tgaccatctg gtgctggccc acatccgtga
    20101 ccaggatcgc gttgccgccg gtgagcgagt tgagtatgtt gatcacctcg cccatagtga
    20161 ggatttcctg ggaaggattg agctctttcc ggatcacctt ggattcttcc tgctccaggc
    20221 actctttgaa ttttttcaac caatccggat aggatttccg ctggatggca tcggtgagca
    20281 tgggaagggt ctctttgcag tctccccaaa ccgggaccgt cgtcttcacg tttttatcga
    20341 tttccgatgg atcgatatcg agatggatga ttttagcctg cttggcatat ttatccagtc
    20401 gtcccgtcac ccggtcatcg aaacgcatcc ccacggcgat caggacatcg cattcattgg
    20461 tgagtagatt gggtccgtaa ttgccatgca tgcccagcat acccacggca ttcggatgat
    20521 cggtagggat ggcgctcatc cccagtatgg tccaggctgc cgggagcccg gatttttcaa
    20581 gaaaggtatc gaattctttt tcggccttgc cgaggatcac cccctgtcca aagatgacca
    20641 ggggcctttc agcctggtct atgagcttgg cagcctcttt gatatattcc ggccggatca
    20701 ccggttttgg ccggtagctg cggatatgtt tgcatttttc ataagcgggg aaggagaagg
    20761 cctggatctg ggcattcttg gtgatgtcga taagcaccgg cccgggacgg ccgcttccgg
    20821 ccaggtagaa agctttggcc aggaccacag ggatttcatg cgcttccgtg acctggtagt
    20881 tccacttggt gatgggtgtg gtaatattca tcacgtcggt ctcctggaag gcatccgttc
    20941 ccagcaaatg cgcgaatacc tgacctgtaa tgcacaccaa tggagtgctg tcgatcatgg
    21001 catcggccag cccggtgacc aggttggtcg cccccgggcc gctggtggcg aaaaccaccc
    21061 cggtccggcc gctggcgcgc gcgtaaccct gggcggcgtg aatggccccc tgctcatgcc
    21121 tgaccaggat atgattgact tcatgctggt gatggaataa ggcatcgtaa atgggcatta
    21181 tagcccctcc gggataaccg aaaatggttt ccaccccttc ggcaatcagg caacgcatag
    21241 tggcttcggc accgctgatc atgacttcgg cctgggtttc agggctggtc tcctgcaagg
    21301 gttgggttat tgaagacata ttatttttat tgtaatgtaa aaatcaaaat tcgtccgtga
    21361 cgcaaccttc gctcgcggag gagacggtca tggcgtattt ttttaaaatt ccggaattgg
    21421 ccttaagggg tggtttggtc cacaagcgca tgcggcggct gatctcttcg tcggaaagga
    21481 gcacatccat gctgtgattc ttaatgtcca ggcggatgat atcgccattt ttgacgaggg
    21541 cgatcgcgcc cccttcctgg gcttccgggg tgacgtgccc gatcacaaat ccatgggtgc
    21601 ctccgctgaa acgcccatcg gtgatgaggg cgaccttatc acccagtccc gcacccatca
    21661 gggcactggt aggtttgagc atttccggca tgcccggggc ccctttgggc ccgcagtagc
    21721 ggatgacaat gacttcaccg ggcttgattt ttttatccag tatggcttgg ttaagatctt
    21781 tctcttcatc aaagacatgg gccgggcctt caaataattc cccttctttt ccggtgatct
    21841 tggccacagc ccctcccggg gcaagattac catataggat ctgaatatgt ccggtagatt
    21901 taatggggct gtcccagggc cggataatgt cctgtcccgc aggcagactg ggtgccgagg
    21961 ccaggttttc cgccagggtt ttgccggtca cggtgaggca gtccccgtgc aaacgaccgg
    22021 catccaataa catttttaac acggcagggg taccgcctat ttcatggagg tcctgcatga
    22081 cgtattttcc gctgggttta agatctgcca ggaaaggggt ccgttcactg atccgctgaa
    22141 aatcatcgat ggtaaggtct acgcttgcag cccgggcgat cgcgatcagg tgcagcaccg
    22201 cattggtgga tcctcccagg accatggtca gggtgatcgc gttttcaaag gcttcctgtg
    22261 tgaggatatc cctgggcctg atgtcttttt caatcaggtt aaatatggcc tttgcaatat
    22321 cctgactttc aagtcttttt gcttcggaaa ctgcagggtt ggatgaattt ccggggaggg
    22381 ccattcccag ggcttcaata gccgaggaca tggtgtttgc cgtgtacatg cctccgcaag
    22441 cccccgctcc gggtatggcg cgatggatca cctcctgcaa gtcttcttcg gtgatcattc
    22501 cattgacttt ctggccgagg gcttcaaaag cggagacgac atcaagcttt ttgccctggt
    22561 aaaaaccggg ggcaatggtc cctccataga ccaggatgga tggccggttg agcctggcca
    22621 tggccatcag ggccccgggc atgtttttgt cacagcccac cacggtgacc agcccatcgt
    22681 accactgtgc tgaaactacg gtctctatgg aatcggctat aatatctctg gagggcaggg
    22741 aataccgcat cccgtcagtt cccatggaaa tcccgtcact gacgccaatg gcatgaaaaa
    22801 taaggccgat catgccctct tcattgattt taccttttat ggaagcggcg attccattga
    22861 ggtgcatgtt gcagggattg ccttcccatc cggtgctggc aatgccgatc tgcggttttt
    22921 tcatgtccgt atccgaaagc ccgatcccgt gaagcatggc ctgggctgcc ggttgagagg
    22981 ggtcctgggt gacgtgtttg ctgtatttat tcaattccat aaatcaattt cccggtgatc
    23041 tttcaaatat tatgctaaat tccttaaccg ccaagaaaag gcgaagacca tcccaaacat
    23101 tcataaaggc ttctacgatg ccaaataatg aaatgggtca tcgcgggata cgaaatccac
    23161 gggaggcttt tacggcaatt gtttcaaccc gggagaaccc ttacctggtc atccccttct
    23221 ttaaacatta aaacgaaaat gcatgacgtc cccgtcctgc accacataat ccttaccctg
    23281 gatcatcagc cggcctgctt cccgggctgc gtgttccgaa ccgtatttaa tataatcggc
    23341 ataactgatg acctcagccc gtataaaacc tttctcaaaa tccgaatgga tcacacccgc
    23401 agcctgggga gccaggtccc cgatgtggat cgtccaggcc ctgacttcct tttccccggc
    23461 ggtgaagtat gagatcaggt tgagtaattt ataacaactg cggatcaccc ggttcactcc
    23521 cggttcttcc agtcccatgg cttccagaaa ttcttttcgt tcttccatgg actccaactg
    23581 ggcgatctgg gcctcaatct cggcactgat gaggatgatc tcggaatgcg catgtccggt
    23641 agccgtgaga ttttcttttg cgacgacggc ttccagggcc cgggtatgtt tattcccgct
    23701 gtggatggat ttctcgtcca cattgcatac gtaaagcacc ggctttgagg tgagcaggaa
    23761 aaaatcgtcg attaagggac ggtcgttttt ttcgcattca aaggtcctgg ccgcttttcc
    23821 tgcatccagg tgttttttga ggttttcggc caattccagc agccggagtt cctctttgtt
    23881 ggctcccttg gcttgttttt tcaaacgatc gatccgtttg tccatggtct ccatgtcctt
    23941 gaacaggagt tccatatcga tgatctcctt gtcccggacc gggtccacag aaccatccac
    24001 atgaatgacg ttgtcgtcct caaagcatcg caccacatga acaatggcct ccacttcccg
    24061 gatattggcc agaaactggt tgcccaggcc ttctccctgt gaggctcctt tgatcaggcc
    24121 cgcaatatcc acgatctcga tggtgttcgg gatggttttc tggggattga ccaattttgc
    24181 cagtgcatcg aggcggggat cgggcaccac aatgatcccg atattcgggt ccttggttgc
    24241 aaaaggataa ttggcagcga gggccttgcc tgacgtgagc gcattgaaca aggtcgattt
    24301 tccgacattt ggcagcccca caatacctcc tcttaaaggc atatttattt ttttattata
    24361 t
//
LOCUS       DAOQXT010000072        24181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2429_length_24181_cov_5.834618, whole genome shotgun sequence.
ACCESSION   DAOQXT010000072 DAOQXT010000000
VERSION     DAOQXT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24181
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2429_length_24181_cov_5.834618"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..949)
                     /locus_tag="PKM27_12770"
     CDS             complement(248..949)
                     /locus_tag="PKM27_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNR08184.1"
                     /translation="MKKLHSTFLTACSLLILSCATLKNQNSEPSTALERLQAGNARFY
                     SHHMKHPDQGPKRVETTALKQQPFAVVVTCSDSRVAPEILFDEGIGDLFVIRNAGNIV
                     EEEDVLASIEYAVVHLGVRTILIMGHEECGAIQAMVAGPDHAGHHEPAHIEAILDKLK
                     KEEEEKAVLDSPARGQERVHQCVVANVMHGIHAVEDTLKSEDMVPKEGLSILGAVYDI
                     HSGKVVFLPAGRSTF"
     gene            1122..1310
                     /locus_tag="PKM27_12775"
     CDS             1122..1310
                     /locus_tag="PKM27_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08185.1"
                     /translation="MKQSEKNELNDRAAAWMITIIVHAAILAALLFVSMPQRQAGPSG
                     EQPKMENPVSGTKIKPKA"
     gene            complement(1336..3498)
                     /locus_tag="PKM27_12780"
     CDS             complement(1336..3498)
                     /locus_tag="PKM27_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNR08186.1"
                     /translation="MQKKLFITLACLCSMTACKQNPSTETTMSNSVFHPERFQAPIAS
                     EKPKELTASGDTRIDPYYWLNERENPEVTAFLEAENKYADSVLAPVKQLKEDLFLELK
                     SRIKEDDSTVPYLKKGYYYYTRFETGKEYPIFCRKKGSLESQEEVLLNVNHLAAGHPY
                     ILAGNIQVSPDDRYMAYIEDTTGRNLFTAYIKDLRTGQLLKDRFETAGGVEWINDSKT
                     IIYETKDPVTLRTNKIWRHRLGSTQDELLFEEKDETAYCSIGKSKSDTYLFLNHGYTQ
                     NIEAHFMSADDPNGSFTLIKPREKDFYYSVEHFGDHFYIMTNKDGAKNFKIMQAPVKS
                     FTVENWKDLIPHDEEVLIESFDIFKNFLVVQERQNGLNQILVRKWADSSSHFIDFGEP
                     TYAAHFGANPEFDTDKLRYVFNSFKTPPSTIDYDMVNKSKEIKKVQAVLGGYDPNQYE
                     TELVWVEAKDKTRVPLSLIYRKGMKKDGTAACYLVGYGSYGSTYDPDFNSNWISLVDR
                     GFVCGIAHIRGGMEMGFKWYEQGKMKNKMNTFTDFIDCAQWLVDQKYTSKDRLFATGR
                     SAGGLLMGAVTNMRPDLFRGIITGVPFVDVLTTMSDPSIPLTTGEYTEWGNPAIKDQY
                     EYMKQYSPYDNLKEGNYPNLYVSTSLNDSQVQYFEPAKYVAKLRKLKKDNNALLLVTN
                     MTGSHGGSSGRFERLHERANEYAFMLGLMGITDAKIRN"
     gene            complement(3517..4149)
                     /locus_tag="PKM27_12785"
     CDS             complement(3517..4149)
                     /locus_tag="PKM27_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNR08187.1"
                     /translation="MQSEPIKHIAIAGNIGAGKTSLATQLARHYRWDVLYEDASDNPY
                     LADFYFDMNRWSFNLQIHFLHSRYRQVLSIRAGTSPVIQDRTIYEDAHIFAPNLFEMG
                     LMSKRDFESYQHLFQTMSSQIAPPDLLIYLRANIPTLVDHIQSRGRDYEGNMSLDYLK
                     KLNKKYDDWIEQYPSELLIVPVDELDFINRSEDLGKIINMVDSKIHGLFS"
     gene            4246..4536
                     /gene="gatC"
                     /locus_tag="PKM27_12790"
     CDS             4246..4536
                     /gene="gatC"
                     /locus_tag="PKM27_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNR08188.1"
                     /translation="MEIGRDLILKLETLAQLELTEEERRQMQGDLQQILQMVDKLQEI
                     NTEGLEPLVYLGADHSPLRTDDKPSSLPAAEALHNARFKEDPYFLVPKVIEK"
     gene            4533..5255
                     /locus_tag="PKM27_12795"
     CDS             4533..5255
                     /locus_tag="PKM27_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR08189.1"
                     /translation="MSALIEIEGLTKTYIMGEDVVHALNGVNLSIYKNEYAALMGPSG
                     SGKSTLMNLMGCLDTPTSGTYHLNGNDVSHMKDRELAQIRNKEIGFVFQTFNLLPRLT
                     ALDNVALPLIYAGMSKSQRIERAEFVLEIVGLEDRMSHKPNELSGGQRQRVAIARALV
                     NNPSIILADEPTGNLDSKTSDEIMRIFERIHNQGNTIILVTHEHDISLHAHRIIRMRD
                     GVVETDQVNPTIVRYEVAAGSR"
     gene            complement(5335..5958)
                     /locus_tag="PKM27_12800"
     CDS             complement(5335..5958)
                     /locus_tag="PKM27_12800"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNR08190.1"
                     /translation="MTVTLLLVIANVIISWQAFQRHDILDKLKHWPYAEKRNREYSRL
                     LTGIFLHGDYIHLALNMYALYSFGTLVEHQFEALFGGTGGKILYLSMYLLSGVFANLM
                     TYHKHQDQPAFASVGASGAVSGIMLSFVIFYPWVKLLLFYIIPIPGILASLLFLLYSS
                     YAARKGHGFIDHTAHFWGAVFGFGFTFLLKPALLPYFINEVIQGISF"
     gene            complement(5977..6450)
                     /locus_tag="PKM27_12805"
     CDS             complement(5977..6450)
                     /locus_tag="PKM27_12805"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNR08191.1"
                     /translation="MKLEVWAIGKTSEAYLSEGIRAYQQKISHFIPFGYQELQESRQQ
                     KSADRSKTMKNQADLVHSRLTPEDHVVLLDEKGKQLNSVQFARYLNGFLISSHKRVVF
                     LTGGAYGFHESVHNKYPEKLSLSSLTFPHDMVRLFLLEQIYRGCTILKNQPYHHI"
     gene            complement(6454..7761)
                     /locus_tag="PKM27_12810"
     CDS             complement(6454..7761)
                     /locus_tag="PKM27_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR08192.1"
                     /translation="MAEPPAQVVKHGNPDWDDQAVMYEVNIRQYTPEGTFTAFSHHVD
                     RLAKMGVDILWLMPIFPISEERRKGSLGSYYSVTDFMETNPEFGTKADFKSLVDKIHG
                     SGMKVIIDWVPNHTGWGHVWIKSHPEYYTQDSAGNIIDPIDPSTGKSWGWTDVADLNF
                     DNKEMRQAHIDAMQYWVSEFDIDGFRYDVAHNVPEDFWVTVSDSLRVMRPLLLLGESE
                     VPAFRNNGSLDLDYGWEFHHILNDIAKGRKNANHIDSFLVKDRMRNTKGYHLYFTSNH
                     DENSWQGTEFERMGDAHQTMAVLAFLFDGMPLLYSGQEEPLRKRLAFFDKDSIEWKNY
                     AYESFYKTLFDLKQNNRALGNGEAGAPLEKLLSHPNVFAFRRTKDNQSVTAIINVTGR
                     RQTISLETDLPGMKEVFTGKVFTYQKGQKIILKPWEYLVYTNP"
     gene            7760..7936
                     /locus_tag="PKM27_12815"
     CDS             7760..7936
                     /locus_tag="PKM27_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08193.1"
                     /translation="MGFVCSRVLQERSRVEVKVKVKVKIKVEVEVEVEAEVKEGRERD
                     PGLNNWWVMFFLTK"
     gene            complement(7939..9564)
                     /locus_tag="PKM27_12820"
     CDS             complement(7939..9564)
                     /locus_tag="PKM27_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08194.1"
                     /translation="MVKADWNYIENFVDPELFEHAYELAEHSNPDVLTLVDKGIYKTR
                     FKSPEGVLIEGEISFGRKYIRQYHCDCQAGKKILCLHLVAATILYRRERLEIPVATQA
                     TLPSKITISSILNQVPKEDLERFLLRYARMDKSFGQALKVHFASRVQVASPEDKYRDL
                     IKSMARLVPNAQGKLPRQAMASLFWISEELLLQAEDLIALDNPAEAFAICHELLCKYA
                     SIYRRLEMYFNDFEKYWIIMHQRLKTILDFPLAPGFRAEMEKKLFDAFADPAYPFIHS
                     PHNLFELLYPLAESAQKKILEDLVLNKIKRNDHNPVPLVSILKTAIKNQDQSLLALAL
                     RNNPEPMRWISALELLVSGNKAYAKSLGPWLISQFQDEYWKLRLMEKLYNWYPEESSS
                     ISYAAHLLLAHPEEKYVRYLLGRGQSVSTIAGLLEQANHPRKNILLTELFLSQDQVDK
                     AKSVIESNPGLDLLKSVTPRFLDLDPEWLTRMYQQLIRAYLDQHIGPVSASQVKSVIS
                     FLRMTKAGTLADQLEKWIKKNFQDHTSLSENLN"
     gene            complement(9784..11475)
                     /locus_tag="PKM27_12825"
     CDS             complement(9784..11475)
                     /locus_tag="PKM27_12825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNR08195.1"
                     /translation="MKFKLVLLSLFTLVGATWAQIQYDPERLKDWHLLDPKSQVPGTG
                     VEEAYKSILKGRTSQEVIVAVIDGGVDIHHEDLKGKIWINPREIPNNGLDDDKNGYID
                     DIYGWNFIGNASGNNVHHDTYESVRTLATLKKKYAQVDPTKLDKVARAEYDRYLSLLK
                     DIEGKRKDAEEQWKELRKNETLLLEVFEAIKKELGPLPLTKNNLEKIDTAKSIYVNIG
                     QKILLSVLEEEPNINSFDQITQSVKQQYGEPRAELEAKFLYQYNTEFDSRTIVGDHYE
                     NPDERYYGNNDVKGPDAFHGTHVAGIIAANRENTIGMKGIARDVKIMALRAVPDGDER
                     DKDVANAIRYAVDHGARVINMSFGKGESPYKKAVDDAIRYAEKRDVLLVHAAGNSGED
                     NDATLHFPVKTLDRKGLFVKKKVRNWLDVGALSFTMDEDMVASFSNFGRKSVDIFSPG
                     VRIYSTAPENRYEFADGTSMASPVIAGVAALIRSYYPKLSSKQVIKAILKNGTEIDQK
                     VNRPGSGDKVSLSQLCKTGSYVNATKALEAASKMKGSRPVPAPAPVPSTMAPPKA"
     gene            11649..12326
                     /locus_tag="PKM27_12830"
     CDS             11649..12326
                     /locus_tag="PKM27_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08196.1"
                     /translation="MKKILGLLFGLSLLTGRSLLAQPVQLGVTAGMTVMQGDYPSYRL
                     KDSFRILANPGFGLFIRRPIGSNAGVKASAYFSSFEGDDSLDPDLNGTRVPSRFKYPF
                     IEIQISGDFSPFHFNLARRQVDFFVLAGAGAVKTTINNTDISDDCPIFNLVIPAGAGM
                     RIHLSEQLQASLHAETNLSFSDCLDGYHGLTNVKDMYTSLKLAISYGIVPEAGKSNGR
                     NIGCPRF"
     gene            12372..14294
                     /locus_tag="PKM27_12835"
     CDS             12372..14294
                     /locus_tag="PKM27_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNR08197.1"
                     /translation="MEEPTPYEILTKYWGYGTFRMRQESIILSILDGKDTLALLPTGG
                     GKSLCYQVPALCREGICLVFSPLISLMNDQVSQLRSKNIPAALVHSGLSLKDTERILE
                     NCLYGEIKLLYLSPERLFSEKAWSRLVRMKVNLIAVDEAHCISQWGYDFRPSYLEIAK
                     IREAMPAVPVLALTATATPEVARDIQQKLLFRQENIVRSSYARSNLAYVVLKEEDKWT
                     RMAAILRKLPGCAIIYVRNRKSAKMVSEYLRQQRISSTYYHAGLSQDERVDRENKWKR
                     NEVRVICATNAFGMGIDKPDVRIVIHLDVPESMEAYFQEAGRAGRDGLKAHAVLFYSS
                     GDRRSMEERLEQNFPDLGFIQQVYKTLAVHYQIAAGSLSEESFPFDLVTFCELKKLPI
                     LPTIEALKVLEHSGLIVLNDGVYQPSLVEMLADKETLYRYQVKHAEEDALIKAILRAY
                     EGVFHHPVRISEYRLGKLLDLHEDDVAYFLASLHRAGILRYQPKSDQSRIFFKGERQK
                     SREVVIDQKWYSFRKTRSRTQLAQIWSYIESEECRSVIMQRYFGETTAEKCKICDHCL
                     QEAGIEENQYLKYKDYVMALLKKKQSIPLQELVDSVSVFRKDKMLQIIRMMESENLVS
                     LDPQMVIRAGDALLAG"
     gene            14330..15457
                     /locus_tag="PKM27_12840"
     CDS             14330..15457
                     /locus_tag="PKM27_12840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR08198.1"
                     /translation="MNSICIFVSNDLVTDQRMQRIAGTLAGAGWKVSLVGRRKKKSVP
                     LILNEVDVKRVNTWFQQGIGFYFMLNVLFFFEALRARTACLMAVDLDTLPAVRLAAFL
                     RNKTMIWDCHEIFSEMPELYKNPLRQKTWKFIESLTVPSLKNVIAVNEGVAAYLEKNY
                     SVKAEVVHNYPLSYPESILPEAKQASGLVLFQGALNEGRCLEVLIRAVQNLDPALRLM
                     IIGEGPLGSHLQSLSRSLELGHRIEFTGELKPEDLRKRTPGAFLGISLLEADHGNSWI
                     SLANKNLDYIMAGLPALTVNFPEYRKINEHWPVAILLDSVTEQTVSNAINRIHHDFTA
                     YKGMHAACLEARKVLCWEKESGKLISFFNGLRVDQDGAFQV"
     gene            complement(15401..16216)
                     /locus_tag="PKM27_12845"
     CDS             complement(15401..16216)
                     /locus_tag="PKM27_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNR08199.1"
                     /translation="MIQSIPLYQVDAFANRLFQGNPAAVCLPGQALPDALMQQIAAEN
                     NLAETAFVIPQGDHFSIRWFTPTVEVDLCGHATLASAFILFFVKSFPGDHIRFDSKSG
                     PLYVSRKDEMLVMDFPADRLEKAPLPEWLPEALGVQILETWTGRSDYLVLVDSEKTLI
                     GIKPDFRMLSSLPGRGLIVTAAGDNLDFVSRCFFPQSGIDEDPVTGSAHTSLVPYWNN
                     KLGKTRFVAAQRSARGGTLYCDLEGDRVHIGGYARLYLEGTVLVDPKAVEEGD"
     gene            complement(16213..17637)
                     /locus_tag="PKM27_12850"
     CDS             complement(16213..17637)
                     /locus_tag="PKM27_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNR08200.1"
                     /translation="MPVPYPDPALYRKHAAELTEIIFQYLSSCREESLPVLETIPPES
                     AYRFWKEELQNPTLSFPALFSKIIQGSTHLHHPGYIGHQVAVPHPYSMLADMAAGLLN
                     NGMAVYEMGQVSVAMERAVCEQIADRLEWNGDPGGIFTSGGSLANLTALLSARQKHSR
                     GKYWEGGHADSPPLAVMVSSEAHYCISRAVKIMGLGRQGLVLLPVNERFQTEVSRLED
                     IYQKTLSQGIEVFAFVANACSTATGAYDDIETIAGFCKKHQIWLHVDGAHGGAVLFSD
                     RHRALLQGAGLADSITVDFHKLLQSSILCTALVYRSIKDSYPTFSQGARYLFEDDEQE
                     WYQTGKRTFECTKAMMVLKAYTLLHQLGETSLGQTIDRQYQLASDFAEMLINHGHFQL
                     ACKPQSNIVCFRYTPPGIPPQNYDLLNREIREELVRGGKFYPVLTTIRDSLYLRTALM
                     NPGTTLDHLASLIDQILAFNPEKL"
     gene            complement(17656..18048)
                     /locus_tag="PKM27_12855"
     CDS             complement(17656..18048)
                     /locus_tag="PKM27_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08201.1"
                     /translation="MKKKEEAKVNKKRIVKDYENLNPELSKALHAAYPAGFAESMISF
                     FDRDGKRVSAIPFETDEIYYLIRMVEKKPKPVKDEADLESETEAEIPPAEEEDLFQDE
                     EEIEEESGDVSLDDIENAENSFSDDDNY"
     gene            18284..19570
                     /locus_tag="PKM27_12860"
     CDS             18284..19570
                     /locus_tag="PKM27_12860"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR08202.1"
                     /translation="MIGGASQNIIALTDSLFLYHHDQLMFAAMGFVGVYYLIIAAIGY
                     GFSRGGQIFIARRMGEKNRLAIGHLTHTMIVFELLIATLMFLLMKFGSEPLFRLLAQS
                     DTIIQHSLEYIQYRSWGIFFSYLGVAYVALYAGVARTSIIGYQTLVLIVVNLILNYGF
                     IFGHFGLPTMGIGGAGLASTLAEAIAVVVFTGFIFLDRKKNPYFLFQIHPINWKSIRQ
                     IVRVSVPFIVQSIVGLGSWFLLFTWIENMGEEELAVSNLGRIAYLIFSVPIWGYAAGI
                     QTMVSGYIGKNKLKAVIPIIWKTARLSLMTTCLIAFPFLILPEFFLYPLLGAEDMHLV
                     YEAKPVFLVILGLLAMFSVATIFFNGLSGTGASFQGLNIQIVCTLIYLLCAFYIIKVR
                     DMGLVWAWASEGIYWLLILFASSAALQTEKWKGIFD"
     gene            19596..20774
                     /locus_tag="PKM27_12865"
     CDS             19596..20774
                     /locus_tag="PKM27_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNR08203.1"
                     /translation="MTEPSRYESRGVSSDKNEVHHAIRRLDRGLFRNTFCKILPNIFS
                     GDPKAALMLHADTAGTKTILAYLYWKETGDLSVWKGIAQDALVMNIDDMACSGPVNRF
                     YISNTVLRNKNRIPGEVLEALIEGTLEFADSLKAHDIHLHHAGGETADVGDVVRTLDV
                     GFTAYSELSRSSVFEINIRPGDLILGIGSFGRSSYEQEYNSGIGCNGLTSARHDVLSG
                     EYGKIYPESFDPSVNPDLVYSGPHQMTDRIETGDGQSRNVGQLLLSPTRTFLPLLYPL
                     LKRAHPGLHGIIHCTGGAHSKVLKFVNGLKIIKDQLFSPPPVFSLIQKCSQASLPEMY
                     KVFNMGTRLEIYGTESCLAEVAAMASELNLPNQIIGRVESAIRNEVELKVGDQGWSYF
                     "
     gene            complement(20778..21254)
                     /locus_tag="PKM27_12870"
     CDS             complement(20778..21254)
                     /locus_tag="PKM27_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08204.1"
                     /translation="MKDFFNKLFGKAKKVGSDIADKAENAFEAAKDKAEDILEDPKVK
                     ATIDKAKAKAADLAEDAKAFGAKVADKAEDAFEVAKDKAEDLWEKTKDKAEDLAVDAK
                     EFGAKVADKADDLWERAKDKAEDLLEKKEAPEANASSSPESKPQDAPGSQEENKPA"
     gene            21359..23002
                     /locus_tag="PKM27_12875"
     CDS             21359..23002
                     /locus_tag="PKM27_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNR08205.1"
                     /translation="MTNKEIANTFELLGKIMELHHENPYKIRSYQNAYLILRKLDRPL
                     KDFTHEELLDLKGVGEAIADKITELLKTGHMATLEKYLAKTPPGVVEMLELQGLGPKK
                     IAILWKELGIESPGELMYACNENRLTAHKGFGEKTQHKIIESIVFLQSNQGKYLFTQA
                     RHHLQALIRRVQEADPDALAEMTGPLRRGLPVVEKAEMITTCSPDLFNQLDPELLTIN
                     KTQAHTLEGRLSGFLPVEIHLTDKKHWASALLTTTGPEHFVKTLPSLKDIPAKKEEDL
                     FHSMAMPYLSPELRDIHGVEKLGKRKLDHLVQSGDLKGLIHCHTTYSDGIHSLSEMAA
                     GAREAGYEYIVITDHSQAAAYANGLKPDRLQEQWKEIDHCNEGRKGFRVLKGIECDIL
                     SSGELDYHDQLLSRFEVVIASIHSAFQMDQAKATARLIRAIENPHTHILGHPSGRLFL
                     SREGYPLDYKKIIDACKANQVAIELNANPQRLDLDYSYIPYCIEKEVYISINPDAHNR
                     GSIRDVDYGITAARKGLLPRELCLNAMESESLLRFCRKG"
     gene            complement(23108..23509)
                     /locus_tag="PKM27_12880"
     CDS             complement(23108..23509)
                     /locus_tag="PKM27_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08206.1"
                     /translation="MKNSIILLLILGLVSWISCEKSSDQEASNGLTGTWNLVAITGGI
                     AGNGYQANFDAIHFDQGSFDLLKNKAKIFSGTYTLNAAADTFKITSTATEADPFLNIK
                     EKKISTDKGNLILSEPCCDLYNYEFSIGSNE"
     gene            complement(23572..>24181)
                     /locus_tag="PKM27_12885"
     CDS             complement(23572..>24181)
                     /locus_tag="PKM27_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNR08207.1"
                     /translation="IDPVVVGAQIVMALQTVVSRQTELTKEAAVVTVATFHAGVRNNI
                     IPEEAELTGTIRTLDRDMQVAIHEKIKRTVSKVAESMGAEAETTISIGNPVTYNDPAL
                     TYQMLPTIQRVAGIHQVHLTKAVTGAEDFSMYALKMPSLFLFVGGMPLGADAAKAPPH
                     HTPDFYVDDRGMKTGVRTLCHLALDYADQWKPEARPSALPGK"
BASE COUNT         5732 a         5942 c         6258 g         6249 t
ORIGIN      
        1 cgaattttca aggactccct tcagtttata ttttttttga atttgaatca aacttcggtc
       61 atcctaccat tggaataata gtagctaaaa taaaaaccga aataggtact cattttcacc
      121 gggacaaatt tatacggatt ttccggattt gttcccgcgg gaaaaaatat ttattgtaca
      181 tatataaaat ttgaaaataa caaaaaaggc tgttttaatt ctttttctct cgcgggaaat
      241 aaccggccta aaaggtgctc cggccggcgg gtaaaaaaac gacctttccg gaatgaatat
      301 catatacggc ccccaggatg gacaggcctt ctttcgggac catatcctcg gatttcagcg
      361 tgtcttcgac cgcatggatt ccatgcatta cattggcgac cacacattga tggacccgtt
      421 cctggccgcg tgcggggctg tccaatacgg ccttttcctc ctcttctttt tttaatttat
      481 ccaggatcgc ttcaatatgg gccggttcat ggtggccggc gtgatcaggg cctgctacca
      541 tagcctggat ggctccgcat tcctcatggc ccatgatcag gatggtccgc acacccagat
      601 ggaccaccgc gtattcaata cttgccagga cgtcttcttc ttccacgata tttccggcgt
      661 tgcggatcac aaagaggtcc ccgatccctt catcaaagag gatttccggg gctacccggg
      721 aatccgaaca ggtgaccacc accgcaaagg gctgttgttt taatgcggtc gtctcaaccc
      781 gtttgggacc ctggtcggga tgtttcatgt gatgggaata aaaccgggca ttccctgcct
      841 gtaagcgttc cagggcggta gacggctcgc tgttttgatt cttaagtgtg gcgcatgaaa
      901 ggattaaaag actgcatgct gtaaggaagg ttgaatgtaa ttttttcata aaaggggtta
      961 actgaaacct ttttctgtta caaaattaaa tacatcccgg gttggtttgg atgccggtgg
     1021 tccggaaagc ttaaataatt tgttaaccca tgatttctgc atttaaccag gcattaatac
     1081 gatttacagg tatttggcac gtttttttca gaaataccgg tatgaaacaa tccgaaaaga
     1141 atgagttgaa cgaccgggca gccgcctgga tgattacaat cattgtacac gccgccattc
     1201 tggccgccct gctctttgtt tccatgcctc agcggcaggc tgggccttcg ggcgaacagc
     1261 ccaaaatgga aaatccggtg agcggcacta aaataaaacc caaggcctga tcacccggtt
     1321 ctgccgacca ccggtctaat tcctgatttt tgcgtcggtg atccccatca ggcccagcat
     1381 aaacgcgtat tcatttgccc tttcatgcaa gcgttcaaag cggccggacg agcctccatg
     1441 tgatccggtc atgttggtga ccaggagcag tgcattgttg tccttcttga gcttgcgcag
     1501 tttagccaca tattttgcag gttcgaaata ctggacctgg gaatcgttca gcgaagtact
     1561 cacatagaga ttggggtaat ttccttcctt caggttgtcg tagggtgaat actgtttcat
     1621 gtattcgtac tggtctttga tggcgggatt gccccattcg gtatattccc cggtagtcag
     1681 ggggatcgag ggatcggaca tggtggtgag cacatccacg aaaggcactc cggtgatgat
     1741 gccccggaat aagtcgggcc tcatgttcgt cacggcaccc atcaacagcc ctcctgcgct
     1801 ccggccggtg gcaaacaacc ggtctttcga agtatatttc tgatccacca gccactgggc
     1861 acaatcgatg aagtccgtga aggtattcat cttgtttttc attttaccct gctcatacca
     1921 tttgaatccc atctccatgc ctccgcggat atgggcgatg ccacaaacaa atccgcggtc
     1981 caccaggctg atccagttgc tgttgaaatc cgggtcataa gtggaaccat aggacccata
     2041 gcccaccagg taacaggctg ccgtgccgtc ttttttcatt cctttccggt agatcaggga
     2101 caggggtacc cgggttttgt ctttggcctc gacccagacc agttccgtct cgtattgatt
     2161 ggggtcatag cctcccaaca ccgcttgtac tttttttatc tccttgcttt tattcaccat
     2221 gtcgtaatca atggtggatg gtggggtttt gaaggaattg aacacatacc gcagtttgtc
     2281 cgtgtcgaat tcgggatttg caccgaaatg agcggcataa gtcggttcgc caaaatcgat
     2341 gaagtgggag ctggaatccg cccattttct gacgaggatc tggttgagcc cgttttggcg
     2401 ttcctgcacc acgaggaagt ttttgaagat gtcgaaactt tcaatcaata cttcttcatc
     2461 atggggaatg aggtctttcc agttttcgac cgtaaacgat ttgaccgggg cctgcatgat
     2521 cttgaaattt ttggccccgt ccttattggt catgatataa aaatgatccc cgaaatgctc
     2581 gacggaataa taaaaatcct tttccctggg cttgatcagg gtaaagctgc cgttgggatc
     2641 atcggcggac ataaaatgag cctcgatatt ctgcgtgtag ccatgattga gaaacaggta
     2701 ggtatccgat ttggacttgc cgatgctgca gtaagccgtc tcgtcctttt cttcgaataa
     2761 taattcatcc tgggtggagc cgaggcggtg cctccagatc ttgttggtgc gcagggtcac
     2821 cggatctttg gtttcgtaaa tgatggtttt ggaatcattg atccattcca cgccgcccgc
     2881 cgtttcaaaa cggtctttca gcagctggcc ggtcctgagg tctttgatat aagccgtaaa
     2941 caaattcctt ccggtggtat cctcgatata agccatgtac cggtcatccg ggctgacctg
     3001 gatgttgccg gcaaggatgt agggatgacc tgccgccaga tggtttacat taagcaatac
     3061 ttcctcctgg ctttcgagac tgcccttctt cctgcaaaaa atcgggtatt cttttccggt
     3121 ttcgaatcgg gtataataat agtacccctt tttgagatag ggcacggtgc tgtcgtcttc
     3181 cttgatccgg gatttcagtt ccaggaacag atcctccttg agttgtttta ccggcgccag
     3241 gacgctgtcc gcatacttgt tttccgcttc caggaaagcg gtcacttcgg ggttttccct
     3301 ttcgttgagc cagtaatagg ggtcgatccg ggtatccccg ctcgccgtaa gttctttggg
     3361 tttttcggag gcaatgggcg cctggaatcg ctcagggtgg aagacgctgt ttgacatggt
     3421 tgtttcggtg gatggatttt gtttacaggc cgtcatactg cagagacagg ccagcgtgat
     3481 gaataatttt ttttgcataa agaagaaaat tacaggtcag gagaacaggc catggatttt
     3541 gctgtctacc atattgatga ttttgcccag gtcttcggac cggttgataa agtccagttc
     3601 gtcaacaggc acgatcagca attcggaagg atattgctcg atccagtcat cgtatttttt
     3661 attcagtttc tttaaataat caaggctcat attgccttcg taatcccggc cccgggattg
     3721 gatgtggtcg accagggtgg ggatattggc cctgaggtag atcaggaggt ccggaggggc
     3781 gatctgggac gacatggtct ggaacaaatg ctgatagctt tcaaaatccc ttttggacat
     3841 caggcccatt tcgaaaaggt ttggagcgaa gatgtgggcg tcttcataga tggtgcgatc
     3901 ctggatcacc ggtgacgttc cggcccgaat ggacaggacc tgccggtacc tgctgtgcag
     3961 gaaatggatt tgcaggttga aggaccaccg gttcatatca aaataaaagt cggcgagata
     4021 aggattgtcc gaagcatctt catataaaac gtcccagcgg tagtggcggg caagctgggt
     4081 ggccagcgac gttttgcctg caccgatatt gccagcaatg gcgatgtgtt tgatcggttc
     4141 tgactgcatt cgggggtgaa agatactatt tcaggcaaaa ttaggtaaga gtttaaaatc
     4201 gtacctttag cgatcatttc agggtactga tctaataaat aaaccatgga gataggccgc
     4261 gatttaattt taaaactcga aaccctggcg caactggagc tgaccgaaga ggaacgccgc
     4321 caaatgcagg gggatttgca gcaaatttta caaatggttg acaaattgca ggaaatcaat
     4381 acggaaggcc tcgagcccct cgtttacctg ggagcggacc acagtcccct gcgtacggac
     4441 gacaagccat cgagcctgcc ggccgcggaa gcccttcata atgcccggtt taaggaagac
     4501 ccctattttc tggtgcctaa agtaattgaa aaatgagtgc attgattgaa atcgaaggac
     4561 tgaccaaaac ctatatcatg ggcgaggatg tcgtacatgc cctgaacggc gtgaacctgt
     4621 ccatttataa gaatgagtac gccgcgctga tgggcccttc cggcagcggc aagtcaaccc
     4681 tgatgaacct tatgggatgc ctcgatacgc ccaccagcgg cacttaccat ctcaatggaa
     4741 acgatgtcag ccatatgaaa gaccgggaac tggcgcagat ccgcaacaag gaaatcggct
     4801 ttgtattcca gacttttaac ttgcttcccc ggcttaccgc cctggataat gtggccttgc
     4861 cgctgattta tgcgggaatg agcaagtccc aacgcatcga gcgggcagaa ttcgtgcttg
     4921 aaattgtagg actcgaagac cggatgtccc ataaaccgaa tgaactttcc ggggggcagc
     4981 gtcaacgggt ggccatcgcc cgggcattgg tcaataaccc gagcatcata ctggccgatg
     5041 agcctaccgg caacctggat tcaaagacca gcgatgaaat catgaggatt ttcgaaagaa
     5101 ttcataacca ggggaatacg atcatcctgg tcacccatga acacgatatt tccctgcatg
     5161 cccaccggat catccggatg cgggatggcg tagtggaaac cgaccaggtc aatcctacga
     5221 tagttcgtta cgaggtggcc gccggctcca ggtaaaagac ggggaactca aagtaaaaag
     5281 ggaacgggtc ctttgagcct gcagaaaaca atcttaccgg accgcttcgc aaggttagaa
     5341 agaaatgcct tggatcactt cattaataaa ataaggtaac aaagcaggtt ttaataaaaa
     5401 agtaaaaccg aaaccaaaaa ccgctcccca gaaatgcgcg gtatgatcaa taaaaccatg
     5461 ccccttcctg gccgcataac ttgaataaag caggaacaaa agggaagcca ggatgcccgg
     5521 aatgggaatg atgtaaaaga gaaggagttt gacccaggga taaaaaatca caaaggacag
     5581 catgattccg gacacggctc cggaagcgcc gacggaagcg aaagcaggct ggtcctggtg
     5641 cttgtgataa gtcatcaggt tggcaaacac cccgctcaac aggtacatgg agaggtaaag
     5701 gattttaccg ccggttcctc caaacaaggc ttcaaactga tgttccacca gggtgccgaa
     5761 gctatacagg gcatacatgt tcagggccag gtggatgtag tcgccgtgca ggaagatccc
     5821 cgtcaacaac ctggaatatt cacggtttct tttctcggcg tagggccagt gtttgagttt
     5881 atccaggata tcgtggcgct gaaaggcctg ccaggagatg atcacattgg cgatcaccag
     5941 taatagggta acggtcatgg gttgagggtc attggattaa atatggtgat agggctggtt
     6001 tttgagtatg gtgcatccgc ggtatatctg ctcaagtaaa aacagacgaa ccatgtcgtg
     6061 gggaaaagtg aggctggata aggacagttt ctcgggatat ttgttatgca ccgattcatg
     6121 gaagccatag gctcccccgg tcaaaaacac cacccgttta tgggaggaaa taaggaatcc
     6181 attcaggtaa cgtgcaaact gaaccgaatt cagctgcttc cctttttcat cgagcaaaac
     6241 cacatggtct tccggagtca gcctggaatg cacaagatct gcctggtttt tcatggtttt
     6301 actcctgtcc gctgattttt gctgcctgga ttcctgcaat tcctggtaac caaaggggat
     6361 aaaatggctg attttctgct gataggccct gatgccctcg gacagatagg cctccgaagt
     6421 ttttccaata gcccagactt ccagtttcat ccgctagggg ttggtgtaca ccaggtattc
     6481 ccagggtttc agaattattt tctgtccttt ctgataggtg aagactttgc cggtgaaaac
     6541 ttctttcatc ccgggaaggt ccgtttccag gcttatggtc tgcctcctac cggtgacatt
     6601 gatgatcgcg gtgaccgact gattgtcttt ggttctccgg aaggcaaata cgttcgggtg
     6661 ggaaagtagt ttttccaaag gagccccggc ttccccgttc ccaagggcgc gattattttg
     6721 tttcaggtca aaaagggttt tataaaaact ttcataagcg taatttttcc attcgatgga
     6781 gtccttgtca aaaaatgcaa gtcttttcct gaggggttct tcctgaccgc tgtaaagtaa
     6841 aggcatgccg tcaaagagga aggccaggac cgccatcgtt tggtgcgcat cacccatacg
     6901 ctcgaattct gtgccttgcc aggaattttc atcgtggttg gaagtgaaat agaggtgata
     6961 accttttgta ttccgcatcc ggtccttcac caggaaactg tcgatatggt ttgcgttttt
     7021 ccggcctttg gcaatatcgt tgaggatatg gtggaattcc catccgtaat caaggtccag
     7081 cgaaccattg ttccggaagg ccggcacctc gctctctcca agaagcagga ggggcctcat
     7141 gacgcggagg ctgtccgaga cggtgaccca gaaatcctcc ggtacgttat gcgcaacatc
     7201 atagcggaat ccatcgatgt caaactcgct gacccagtat tgcatggcgt cgatatgtgc
     7261 ctggcgcatt tctttgttgt caaagttgag gtcggccaca tcggtccagc cccaggattt
     7321 ccccgtggaa ggatcgatcg ggtcgataat attgccggcc gaatcctggg tataatattc
     7381 cggatgggat ttgatccaaa catgtcccca gcccgtatga ttgggaaccc agtcgatgat
     7441 caccttcatc cccgacccat ggattttgtc caccaaggat ttaaaatcgg ctttggttcc
     7501 gaattcggga ttggtctcca tgaaatcggt gacgctgtaa taactgccca ggctgccttt
     7561 ccttctttct tcactgatcg ggaaaatggg cataagccac agaatatcca ctcccatttt
     7621 tgccaaacga tccacatgat ggctaaaagc agtaaaggtg ccttccggtg tgtactgccg
     7681 gatgtttacc tcatacatga ccgcctggtc atcccaatcc ggattgccat gtttgaccac
     7741 ctgagccggt ggttcagcca tgggttttgt ttgttcccgg gttttgcagg agaggagtag
     7801 ggtggaggtt aaggttaagg ttaaggttaa gattaaggtt gaggttgagg ttgaggttga
     7861 ggctgaggtg aaggagggga gggaaaggga tcccggtctg aataattggt gggtcatgtt
     7921 tttcttaaca aaataaggtt aattcaggtt ttcggacagg ctggtatggt cctgaaaatt
     7981 tttttttatc catttttcaa gttgatccgc cagggtaccg gctttcgtca ttctcaggaa
     8041 tgaaatgacg gatttgacct ggctggcgga aacagggcca atgtgctggt ccaggtaggc
     8101 ccggatcaat tgctggtaca tccgggtcag ccactctgga tcgaggtcga ggaagcgcgg
     8161 ggttacactt tttaacaggt caaggcccgg attggattcg atgaccgatt ttgccttgtc
     8221 cacctggtcc tgggagagaa aaagctcggt cagcaggatg ttttttctgg ggtggttggc
     8281 ttgttcgagc aagccggcta tcgtggaaac agactggccc cggcccagga ggtacctcac
     8341 atatttttcc tccggatgag caagcagcaa atgagcggcg taggatatgg aagaactttc
     8401 ctccgggtac caattgtaca atttctccat cagccggagt ttccagtatt catcctggaa
     8461 ctggctgatg agccaggggc ccagggattt tgcataggct ttattgccgg aaaccagtaa
     8521 ttccagggcg gagatccacc gcatgggctc cgggttattt ctcaatgcca gggccaaaag
     8581 ggactggtcc tggtttttaa tcgctgtttt aagaatggat accaggggga cgggattgtg
     8641 gtcgtttctt ttgattttgt tcaggaccag gtcttccagg atcttttttt gggctgactc
     8701 agccaatggg tacaagagct caaaaaggtt atgaggagaa tgaataaacg ggtaagcggg
     8761 gtccgcgaat gcatcaaata actttttttc catctccgcg cggaatcccg gtgcgagggg
     8821 gaagtcgaga atggttttca gccgctggtg catgatgatc cagtattttt caaagtcgtt
     8881 gaagtacatc tcgagccgcc ggtagatcga agcatatttg cataataatt catggcagat
     8941 cgcgaaggcc tcagcagggt tgtccagggc gatcaggtct tcggcctgca acagcaattc
     9001 ctcgctgatc caaaacaggg atgccatagc ctgccggggc aattttccct gggcgttagg
     9061 caccagcctg gccatacttt tgatcaggtc ccggtatttg tcttccggag aggcaacctg
     9121 gacccggctt gcaaaatgta ctttcaaggc ctggccaaag gatttatcca tccgggcgta
     9181 gcggagcaaa aagcgttcga ggtcttcctt cgggacctgg ttgaggatcg aagatatcgt
     9241 gatctttgat ggcagggtgg cctgggtggc tacaggaatt tccaacctct ccctccggta
     9301 caaaatggtg gcggcgacca ggtgtaaaca caagattttt ttgcctgcct ggcaatcaca
     9361 atggtattgg cgaatatatt tccttccgaa cgaaatttcg ccttcgatca ggaccccttc
     9421 cggggattta aaccgggtct tgtagattcc cttatcgacg agcgtgagta cgtcgggatt
     9481 cgaatgctca gcaagttcat aggcatgctc aaacagttcc ggatccacaa aattctcaat
     9541 atagttccag tccgctttga ccatagttta acctttaaag ataggccgaa tagaggaaag
     9601 tacaaaaagg gcaagcggtt gctggcataa atcggggtct cgcgtcagct gagcaaagga
     9661 ggataggccg cgccagtcaa ttaggatcct accaatccta ccaatcctac caatcctaaa
     9721 aatcctatta atcctattaa tcctaaaaat cctattaatc ctatcaatcc tatcaatcct
     9781 atcctacgct ttcgggggcg ccatagtaga aggaaccggg gctggagcgg gcacgggccg
     9841 tgaacctttc attttcgagg ccgcttcgag ggctttggtc gcattgacgt aagaacctgt
     9901 tttacacaat tggctcaggg agactttatc ccctgacccc ggcctgttga ctttctggtc
     9961 gatttccgtg ccgtttttaa gaatggcttt gatgacctgc tttgagctga gtttagggta
    10021 ataggacctg atcagggcag cgacgcccgc aatgacgggg gatgccatgc tggtgccgtc
    10081 tgcaaattca tatctgtttt caggagcggt gctgtagatc ctgactcccg gggaaaaaat
    10141 gtccacggat tttctgccga aattggaaaa agaggctacc atgtcttcat ccatggtgaa
    10201 ggacagggca cccacatcca gccagttccg gaccttcttt ttgacgaata atcccttgcg
    10261 gtccagtgtt tttaccggga aatgcaaggt ggcatcattg tcctctcccg agtttccggc
    10321 ggcatgcacg agcaggacat cccttttttc ggcataacgg atggcatcgt ccaccgcttt
    10381 tttataaggg gattcccctt tgccaaaact catattgatg acccgggctc cgtggtccac
    10441 ggcgtaacgg atggcattgg ccacatcttt gtccctttca tccccgtcgg gcaccgcccg
    10501 gagcgccatg attttgacat ccctggcgat tcctttcatg ccgatggtat tttcccggtt
    10561 ggcggcaatg attcccgcca cgtgcgtgcc gtggaaagca tccggccctt ttacgtcgtt
    10621 gttgccgtaa tatctttcgt cggggttttc gtaatggtca cccacgatgg tacgggagtc
    10681 aaactccgta ttgtattgat atagaaattt ggcttccagt tcggcacggg gttcaccgta
    10741 ttgttgtttg acagattggg tgatctggtc gaatgaattg atgttcggtt cttcttccag
    10801 tacgctgagc aggatcttct ggccgatgtt gacataaatg ctcttggccg tatctatctt
    10861 ttccagattg tttttggtca gcggaagggg cccgagttct tttttgatgg cttcgaaaac
    10921 ttcgagcagg agggtctcat tttttctcag ttctttccat tgttcttcgg catccttcct
    10981 ctttccttct atatccttga gaaggctgag gtagcggtca tattcggcac gggccacctt
    11041 atccagtttg gtcggatcga cctgggcgta ttttttctta agggtggcca gcgtgcgcac
    11101 gctttcatag gtgtcgtggt gcacattatt gcccgatgca tttccaataa aattccagcc
    11161 atagatatcg tcgatatatc cgtttttatc atcatccaag ccattattgg ggatctcacg
    11221 cgggttgatc catattttgc ctttcaggtc ttcatgatgg atgtccactc cgccatcaat
    11281 gacggccaca atgacctcct gggaggtcct gcctttcaga atggatttat aggcttcctc
    11341 aacccccgtt ccgggaacct gggacttagg gtccaggaga tgccagtctt tcagtctttc
    11401 cgggtcgtat tggatttgag cccaggtagc accgaccagc gtaaacaggg agagcaggac
    11461 aagtttaaat ttcataccac aaataaccag aattcattaa tttttgatgg tccatttgcc
    11521 ttttttaaca tataattcac caagaccttt gtattcctga ggccgcagga aataaggccg
    11581 gccggaattc atatttttgc agccctttga taccttggca ttattttgga acgttaggct
    11641 tgtattcaat gaaaaaaatt ctggggcttt tatttgggct ttccctcctc accggccggt
    11701 ctctcctggc ccagcctgtt caactggggg tgactgccgg gatgacggtc atgcaggggg
    11761 actatccttc gtaccggtta aaggacagtt tcaggattct tgccaatccg gggttcggtc
    11821 ttttcatacg gaggccgatc ggctcaaatg caggggtgaa agcatccgcc tatttttctt
    11881 cttttgaagg ggatgacagc ctggaccctg atctgaatgg gaccagggtc cccagccgct
    11941 ttaagtaccc gtttatcgaa attcagatct ccggagattt tagtcctttt cattttaatc
    12001 tggcccggcg acaagttgat tttttcgtcc tggccggtgc gggggccgtg aaaaccacca
    12061 tcaacaatac cgatatcagc gatgattgcc ccatatttaa cctggtgatc ccggccggtg
    12121 cgggcatgcg gatccatttg tccgaacagt tacaggcctc ccttcacgcc gaaaccaacc
    12181 tgagtttcag cgattgcctg gatggctatc acggacttac caatgtgaag gacatgtaca
    12241 cctctctgaa actggccatc tcttacggta tcgtgccgga ggccggtaaa agcaatggcc
    12301 ggaatatcgg ttgccccagg ttctgaaatt ggaagggccc gaaaattccc tgatctttgg
    12361 acaggacata aatggaagag cccactccat atgaaatttt aaccaaatac tggggttatg
    12421 ggaccttccg gatgcggcag gaatccatca tcctgtccat cctggatgga aaagataccc
    12481 tggccttgct tcccacaggc ggcggaaaat ccctgtgtta ccaggtgcct gccctctgcc
    12541 gggaagggat ttgcctggtt ttttctccgt tgatttcgct catgaatgat caagtgagcc
    12601 agcttaggtc aaaaaatatt ccggcggccc tggtccattc gggactctcc ctgaaggata
    12661 ctgaacgtat cctggaaaac tgcctgtatg gtgagatcaa actgctttat ctatccccgg
    12721 agcgcttgtt ttccgaaaaa gcctggtccc ggctggtccg gatgaaggtc aatctgatcg
    12781 cggtggacga ggcccattgc atcagtcaat ggggctatga tttcaggccc tcctaccttg
    12841 aaatagcgaa gatccgggaa gccatgccgg ccgttccggt cctggccctg accgccaccg
    12901 ccacccctga ggtggcaaga gacatccagc aaaagcttct tttccggcag gaaaacatcg
    12961 tccggtccag ttatgccagg agtaacctgg cctacgtggt gctcaaggaa gaggataaat
    13021 ggacccggat ggcggctatc ctccggaaac tcccgggatg cgccattatt tatgtacgca
    13081 accgcaagtc agccaagatg gtcagcgagt atttgcggca acaacgcata tccagtacct
    13141 attatcatgc aggtctgagc caggatgaac gggtcgaccg ggaaaacaaa tggaaaagaa
    13201 atgaagtgag ggtcatttgc gccaccaatg cctttggcat gggtattgac aaaccggacg
    13261 tccgcatcgt catccatctg gatgtaccgg aaagcatgga agcttatttc caggaggcag
    13321 gtcgggcggg tcgtgacggc ctgaaggccc atgccgtttt attttacagt tcaggggacc
    13381 ggcggagcat ggaggagcga ctggagcaaa actttcctga cctcggcttc atacaacagg
    13441 tgtacaaaac ccttgccgtg cattatcaaa tcgcggccgg cagcctctcc gaagaatcct
    13501 ttccgtttga cctggtgact ttttgcgaat tgaaaaaatt gcccatcctg cccaccatcg
    13561 aggcgctgaa agtcctggag cacagtgggc tgatcgtgct caacgacggg gtctaccagc
    13621 cgtccctggt cgaaatgctt gccgataaag agacccttta ccggtaccag gtaaagcatg
    13681 cggaagaaga tgccctgatc aaagccatcc tgcgcgctta cgaaggggtt ttccatcatc
    13741 cggtcaggat ctcggaatac cgcctaggta aattgcttga cctgcacgaa gacgacgtcg
    13801 cctatttcct ggcttcgctg caccgggcag gcattctccg ctaccagccc aaatccgatc
    13861 agtccaggat tttctttaaa ggggaaagac agaaaagcag ggaagtggtc atcgatcaga
    13921 aatggtattc tttcaggaaa acaaggtcca ggacccagct tgcccagatc tggtcctata
    13981 ttgaatcgga ggaatgccgc agcgtaatca tgcaaaggta ttttggcgaa accaccgctg
    14041 aaaaatgtaa aatctgcgac cattgcctcc aggaagcggg gattgaagaa aatcaatatc
    14101 tcaagtacaa ggattatgtc atggccttgt tgaaaaaaaa acaatccatt ccgctccagg
    14161 agctggtgga ttccgtttcg gttttcagga aggataaaat gctccagatc attcgcatga
    14221 tggaaagcga aaacctggtc agcctggatc ctcagatggt gatccgggcc ggagatgccc
    14281 tgctcgcagg ctgaagccca aatccgcccg acttcttaca tttatcccgg tgaactccat
    14341 atgcattttt gtttccaatg acctcgtcac ggaccaacgc atgcaacgga ttgcaggtac
    14401 tcttgcgggt gccgggtgga aggtaagcct ggtcggcaga agaaagaaaa aatcagtccc
    14461 cctgatcctg aatgaggtgg acgtaaaacg ggtgaatacc tggtttcagc agggcattgg
    14521 tttttacttc atgctcaatg tgcttttttt tttcgaagcc ttgagggcgc gcaccgcttg
    14581 tctgatggcg gtggatctgg ataccctccc ggccgtccgc ctggccgcct tcctgcggaa
    14641 taaaaccatg atctgggatt gtcatgaaat ttttagtgag atgcctgaat tgtacaaaaa
    14701 ccccttaagg cagaaaacct ggaaattcat tgaaagcctt acggtcccgt cgctaaagaa
    14761 tgtcatagcc gtgaatgaag gggtggcggc ctaccttgaa aaaaattact ccgtcaaagc
    14821 ggaggtggta cacaattatc ccctctctta cccggagtcg atccttccgg aggccaagca
    14881 ggcttcgggc cttgtactct ttcagggcgc gctcaatgaa ggcaggtgcc tggaggtgct
    14941 catccgtgca gtccaaaatt tggatccggc tttgaggttg atgatcatcg gcgagggccc
    15001 tttaggttcc cacctgcaat ccttgagccg ctccctggaa ctggggcacc ggattgagtt
    15061 tacaggagaa ctgaagccgg aagatttgag aaaaaggaca cccggcgcat ttctgggaat
    15121 cagcttgctt gaggcggacc atggcaattc ctggatttcc cttgccaata aaaacctgga
    15181 ttatatcatg gcgggccttc cggcattgac ggtgaatttc ccggaatatc gcaagatcaa
    15241 tgaacattgg ccggtagcca tcctgctaga ctcggtcacg gaacaaaccg tttcaaatgc
    15301 aatcaaccgg attcaccatg attttacagc ctataaaggc atgcatgccg cttgcctgga
    15361 agcccgaaag gtcttgtgct gggaaaagga atcggggaag ttaatctcct tcttcaacgg
    15421 ccttagggtc gaccaggacg gtgccttcca ggtatagccg ggcatacccg ccgatatgga
    15481 cccggtcacc ctccaaatca cagtacaagg tgccgccccg ggcagatctc tgggccgcta
    15541 cgaaccgggt ctttccaagt ttgttattcc aataagggac cagggaagtg tgggcgctgc
    15601 ccgtcaccgg gtcttcatca attcccgatt gggggaaaaa gcacctggag acaaaatcaa
    15661 gattatcgcc ggccgccgtc acgatcagcc cgcggcccgg cagtgatgag agcattctga
    15721 aatccggttt tatcccgatt aaggtttttt ctgaatccac caggaccagg taatcagacc
    15781 ttcccgtcca ggtctcaagg atttggacgc cgagcgcctc cggcaaccat tcgggcaagg
    15841 gggctttttc cagacgatcc gccgggaagt ccatcacgag catttcatcc ttccggctta
    15901 catagagcgg accgcttttg gagtcaaagc ggatatggtc gccgggaaag gacttgacaa
    15961 aaaataaaat gaaagcgctg gccagggtgg catggccgca aaggtccact tccaccgtgg
    16021 gcgtaaacca gcggatcgaa aaatgatctc cttgcggaat tacaaaagcg gtttcggcga
    16081 gattattttc agcggcgatc tgctgcatca gtgcatcggg aagggcctgg cccggtaaac
    16141 aaaccgcggc aggattgccc tggaacaatc ggttggcaaa ggcatctacc tggtaaaggg
    16201 ggatggactg gatcataatt tttccggatt aaaagcgagg atttgatcta ttaaactggc
    16261 caggtggtcc agggtggttc ccggattcat taaggcggta cgcaaataaa gggaatcccg
    16321 tatggtagtc aagaccgggt aaaattttcc tccgcgcacc aattcctccc ttatttccct
    16381 gttgagcagg tcatagtttt gaggcgggat cccgggcggg gtgtaccgga agcagacgat
    16441 attggattgg ggtttgcagg ccaattgaaa atggccgtga ttgatgagca tttcggcaaa
    16501 atcggaagcc agctgatatt gccggtcaat ggtttgcccc agggatgttt ctccgagctg
    16561 atggagcaag gtatatgctt ttagcaccat catcgccttg gtacattcaa aagttctttt
    16621 cccggtctgg taccattcct gttcatcgtc ttcaaacaaa taccgggcac cctgggagaa
    16681 cgtcggatag gaatccttga tcgaccggta aaccagggcc gtgcaaagga tggaactctg
    16741 cagcagtttg tgaaaatcca cggtgatcga atccgccagc ccggccccct gaagcagggc
    16801 tcggtgacgg tcactgaaca gaaccgcccc tccgtgggct ccatcgacat gcagccagat
    16861 ctggtgtttt ttgcaaaagc ctgctatggt ttcaatatcg tcgtaagccc cggtcgcggt
    16921 actgcatgca ttcgccacaa aggcaaatac ttcaatgccc tggctcaggg tcttttgata
    16981 aatgtcttcc agccgggaca cctcggtctg aaaccgttca ttaaccggaa gaagaaccag
    17041 gccctggcgg cccaagccca tgattttaac cgcgcgggaa atacaataat gggcttcgga
    17101 ggataccatg acggcaagcg ggggcgaatc cgcatggccg ccttcccaat atttgccccg
    17161 ggagtgcttt tgcctggccg ataaaagcgc ggtcaagttt gccagggaac cgccggaggt
    17221 gaatataccg cccggatccc cgttccattc aagccggtcg gctatttgtt cacagaccgc
    17281 acgttccata gccaccgaaa cctggcccat ttcataaacc gccatcccgt tgttgaggag
    17341 cccggcggcc atatcagcca acatggagta gggatgaggt accgcaacct ggtgcccgat
    17401 ataaccgggg tggtggaggt gggtggatcc ctggataatt tttgaaaaca aggccggaaa
    17461 ggataaagtc gggttttgca gctcttcttt ccagaaccgg taagcgcttt ccggagggat
    17521 ggtttccagc accggaaggg attcctccct gcaagaggag aggtattgaa atatgatttc
    17581 cgtgagttct gctgcgtgtt ttcggtataa tgcaggatcg gggtagggaa cgggcaaaac
    17641 agggtggata tttactcaat agttgtcgtc atccgaaaaa gaattttcgg cattttcgat
    17701 gtcatccagg gagacgtcgc ccgattcttc ctctatttct tcctcgtcct ggaaaaggtc
    17761 ctcttcttca gctggcggga tttcggcttc ggtttcggac tccaggtcag cttcgtcttt
    17821 caccggtttt ggttttttct caaccatcct gatgaggtaa taaatttcat ccgtttcaaa
    17881 gggtatggcg gacacccgtt ttccatcgcg gtcaaaaaaa gaaatcatgg attcggcaaa
    17941 gccggccggg tatgccgcat gcaatgcttt agagagttcc gggttaaggt tctcatagtc
    18001 ctttactatt cttttcttgt tgacctttgc ttcttctttt ttcttcataa aagaatagat
    18061 tgtttcattt gataattgcc ttcaatgact gatcctgatc aggcaaattt atatgcactt
    18121 ccttatttct tttcattttt ctctgaataa tttttttttc tcaaaaagcc gcctgtgcgc
    18181 ggattctgcc ggccggatat aaggaattga gtttattttt gccagccaca aggtgaatct
    18241 cagtacctcg tatcgtaata ttttcaagat atccgcaccc atcatgatcg gtggggcttc
    18301 acaaaatatt atagcgctta ccgacagcct gtttttgtat catcatgatc aactgatgtt
    18361 tgcggccatg ggcttcgtag gcgtttatta tctgatcatt gccgccatag gttacggttt
    18421 ttccagaggg gggcagattt ttatagcccg gaggatgggt gagaagaaca ggctggccat
    18481 cggccacctg acccatacca tgatcgtttt cgaactgctc atcgcaaccc tgatgttttt
    18541 gttgatgaaa ttcgggagcg agccgctgtt caggttgctg gcccaatcgg ataccatcat
    18601 tcaacacagc cttgaataca tccaatacag gtcctggggc atttttttca gttacctggg
    18661 tgtagcctac gtcgctttgt atgcgggggt ggccaggacc tcgattattg gctaccagac
    18721 cctagtgctg atcgtggtga acctgatttt gaattacggg tttattttcg gccattttgg
    18781 cctcccgacc atgggtatag gcggagccgg gcttgcgagt acgcttgccg aagccattgc
    18841 cgtagtggtc tttaccggct ttatttttct ggaccggaaa aaaaatcctt atttcctttt
    18901 ccagattcat cccatcaact ggaaatccat ccggcagatc gtaagggttt cggtcccatt
    18961 tatcgtccag tccattgttg gcctgggctc ctggttttta ttgtttacct ggattgagaa
    19021 catgggcgaa gaggaacttg ccgtctccaa cctcggccgg atcgcttacc ttattttcag
    19081 tgtaccgatc tggggttatg cggcggggat ccaaaccatg gtcagcgggt atatcggcaa
    19141 gaacaaacta aaagcggtga tcccgatcat ctggaagacg gccaggttgt ccctgatgac
    19201 cacctgtctc atcgccttcc ccttccttat cctgcctgag ttcttcctgt accccttgct
    19261 gggcgcggaa gacatgcacc tggtctatga ggctaagccc gtctttttgg tcatcctcgg
    19321 gctgctggcg atgttttcgg tggccactat ttttttcaat gggctcagcg gcaccggggc
    19381 ctctttccag ggcctgaata tccagatcgt atgcaccctc atttacctgc tttgcgcttt
    19441 ttacatcatc aaggtccggg atatgggcct ggtctgggcc tgggcctcag aaggaatata
    19501 ctggctgctg atcctctttg cttcgtccgc agccctgcag actgaaaaat ggaaaggaat
    19561 atttgattaa aaatgttttt atagctttac ctggcatgac cgagccttcc agatatgaat
    19621 cccgcggtgt ttccagcgat aagaatgaag tgcatcatgc catccgccgc ctggaccggg
    19681 gattgttccg gaacaccttt tgcaagatcc ttccaaatat tttttctggt gatccgaaag
    19741 cagccctgat gttgcatgcc gataccgccg gcaccaagac catccttgcc tatctttatt
    19801 ggaaagaaac cggcgacctt tcagtatgga aagggattgc ccaggatgcc ctggtcatga
    19861 atattgatga catggcctgc agcgggccgg tcaaccggtt ttatatttcc aataccgttc
    19921 tgcgcaataa aaaccggatc cctggcgaag tcctggaagc cctgattgaa ggcactctgg
    19981 agtttgcgga ttccctgaag gcccatgaca tccacctgca ccatgcagga ggggaaacgg
    20041 cggatgtcgg cgacgtggtg cggacccttg atgtcgggtt tacggcctat tcggaacttt
    20101 ccagatcctc ggtgtttgaa atcaatattc gtcccggtga cctgattctc ggtattggtt
    20161 ccttcggacg ttccagttac gaacaggaat acaacagcgg gattgggtgc aacgggctca
    20221 ccagcgcaag acatgatgta ttgtccgggg aatatggaaa gatctacccc gaatcttttg
    20281 atccgtcggt gaatccggat ctcgtttact ccgggccaca ccagatgact gaccggatcg
    20341 aaaccgggga tggacagagc cggaatgtgg gacagctgtt gctctcccca accaggacct
    20401 tcctgcccct cttgtatccc ctgttgaaac gggcgcaccc cggtttacac ggcattattc
    20461 actgcacggg gggggcgcat tccaaggtcc ttaagtttgt aaatggatta aaaatcatca
    20521 aggaccaact gttttcaccg ccccctgttt tttccctgat tcaaaagtgc agccaggctt
    20581 ccctcccgga aatgtataag gtcttcaata tgggaacccg cctggaaatc tacggtacgg
    20641 aatcctgtct ggctgaagtg gcggcaatgg ccagcgaact gaatttgccc aatcaaatca
    20701 ttgggcgggt ggaatcggca atccggaatg aggtcgaatt aaaagtggga gaccagggct
    20761 ggtcctattt ttagggctca ggctggttta ttttcctcct gcgagccggg ggcatcctgg
    20821 ggtttcgatt ccggagagga agaagcgtta gcctccgggg cttccttttt ttccaacagg
    20881 tcctccgctt tgtctttggc cctttcccaa agatcatctg ctttatcggc cacttttgct
    20941 ccgaattcct tcgcgtccac cgcaagatct tcggctttgt cttttgtttt ttcccagagg
    21001 tcttctgctt tatccttagc cacttcaaag gcatcttcgg ctttgtcggc cacttttgca
    21061 ccaaaggcct tggcatcttc agccagatcg gcggctttgg ccttggcttt atcgatggtg
    21121 gctttcactt tgggatcctc caaaatatct tccgccttgt ccttggcggc ttcaaaagcg
    21181 ttctctgctt tatcagcgat gtctgaacct actttcttcg ctttgccaaa aagtttgtta
    21241 aaaaaatctt tcatatttcg ctttgtttaa caggatataa tattacaaag atccctacaa
    21301 gttaggcaat tttattttat gggatgataa aattggccgc agcaagtacc tttacgcggt
    21361 gaccaataag gaaatagcaa acacctttga attgttgggt aaaatcatgg agttgcacca
    21421 tgagaatccc tataaaataa ggtcctacca gaatgcttat ctcatcctga gaaaactgga
    21481 caggcctttg aaagatttta cccacgaaga attactggac ctcaaaggcg tgggagaagc
    21541 cattgcggac aaaatcaccg aattgctgaa gaccgggcat atggccaccc tggaaaaata
    21601 tttggccaaa acacctccgg gcgtggtgga aatgctggag ctccagggcc tcgggcccaa
    21661 gaaaatagcc atcctgtgga aagaactggg tattgaaagc ccgggcgaat tgatgtatgc
    21721 ctgcaatgaa aaccggctga ccgcgcacaa gggatttgga gaaaaaaccc agcacaaaat
    21781 cattgaatcc attgtcttcc ttcaatccaa ccagggaaaa taccttttta cccaggcccg
    21841 gcatcatctt caggccctga tcagaagggt ccaggaagca gaccccgatg ccctggcaga
    21901 aatgaccggc cctttgcgaa gaggcctgcc ggtcgtggaa aaagcagaga tgatcaccac
    21961 ctgttccccg gatttattca atcagttgga cccggaattg ttgaccatca ataaaacaca
    22021 agcccatacc ctggaaggcc ggctcagcgg cttcctgccc gttgaaatac acctgacgga
    22081 taaaaaacat tgggcgtccg cactgctcac taccaccggc ccggagcatt ttgtcaaaac
    22141 ccttccatct ctgaaggata tccccgcaaa aaaggaagag gatctttttc attccatggc
    22201 catgccatac ctttcccctg aattaaggga tatccatggt gttgaaaaac ttggtaaaag
    22261 gaaactggat cacctggttc agtccgggga cctcaagggc ctgatccatt gccataccac
    22321 ctatagcgac ggcattcaca gcctgtccga aatggccgcc ggagccaggg aagccgggta
    22381 tgagtatatc gtgatcacgg accattcaca ggctgccgcc tatgccaatg gactcaaacc
    22441 cgatcgactc caggagcaat ggaaggaaat cgatcactgc aatgaaggcc gtaaaggatt
    22501 cagggtgctc aaaggcattg aatgcgatat cttgtcgagt ggtgaactgg actaccatga
    22561 ccagctgttg tcccgctttg aggtggtgat tgcgagtatt cactccgctt tccagatgga
    22621 tcaggccaaa gccaccgccc ggttgatccg ggccattgaa aatccgcata cccatatcct
    22681 cgggcatccg tcaggccgcc ttttcctctc cagggagggt tatcccctgg attacaaaaa
    22741 gatcattgac gcctgcaagg ccaaccaggt agcgattgaa ctcaatgcca atccgcaacg
    22801 cctggacctc gattattcct atatcccgta ttgtattgaa aaggaagttt atatttccat
    22861 taacccggat gcgcacaacc gcggctcaat ccgggatgtc gattatggga tcaccgcggc
    22921 ccggaaaggg cttctgccta gggaactctg cctgaatgcc atggagtcgg aatcgctatt
    22981 aaggttttgc aggaaggggt agagggtaga ggattgagga ttgaggattg aggattgagg
    23041 attgaggatg gaggtcagag tgacatgccc actcgtacct cattcctcaa tcctcaaacg
    23101 tcatgcctca ttcattcgaa cctatgctga attcgtagtt gtaaaggtcg cagcagggct
    23161 cgctgagaat gagattccct ttgtcggtgc tgatcttttt ctccttgata ttgaggaaag
    23221 gatcggcttc ggtggccgtg ctggtgattt taaaggtatc ggcagcagca ttaagggtat
    23281 aggttccgga gaagattttt gctttgtttt tcaaaaggtc aaaactcccc tggtcaaagt
    23341 gaatggcgtc aaaattggcc tgatagccgt tccccgcaat accaccggtg atcgccacca
    23401 gattccaggt gccggtcaaa ccattagagg cttcctgatc ggaagatttt tcacaagaaa
    23461 tccaggacac cagtcccagg attaatagaa ggatgattga atttttcata atcagataga
    23521 cggtggaagg atgcgggagg ttgggtatgg ccggattttt ttggtaaggg attatttacc
    23581 cggtaaagcg cttggcctgg cctccggctt ccactgatcc gcataatcca gggccaggtg
    23641 gcagagggtc cggaccccgg ttttcatacc gcggtcatcc acgtaaaaat caggggtgtg
    23701 atggggaggg gctttggccg cgtccgcacc aaggggcatc cctcctacaa aaaggaaaag
    23761 cgagggcatt ttgagcgcgt acatagaaaa atcctccgca ccggtcaccg ccttggtaag
    23821 atggacctgg tggatgccgg ccacccgctg aatggtcgga agcatctggt aggtgagggc
    23881 aggatcattg taagtcaccg ggttgccaat ggagatcgtg gtttcggctt ctgcccccat
    23941 gctttcggcg accttcgaga ccgtccgttt gattttttca tgaatggcca cttgcatgtc
    24001 acggtccagg gtccggatgg tgcccgtcag ttctgcttct tccgggatga tgttgtttcg
    24061 aaccccggcg tgaaaagtgg caaccgtgac cacggcggcc tccttggtca gttcggtctg
    24121 ccggctgacc accgtctgaa gcgccatcac aatttgtgca ccgaccacca ccggatcgat
    24181 c
//
LOCUS       DAOQXT010000073        23772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2482_length_23772_cov_5.887549, whole genome shotgun sequence.
ACCESSION   DAOQXT010000073 DAOQXT010000000
VERSION     DAOQXT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23772
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2482_length_23772_cov_5.887549"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKM27_12890"
     CDS             <1..810
                     /locus_tag="PKM27_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR08208.1"
                     /translation="VTVVEFMEQGLVPREDEDISKELSRAFNKSGIRVHANTEVTRVD
                     TSGPGCLVYLKNRKTGQEESLACDVVLSAAGITPNLENLGLETAGVQLEKGLVKVDGY
                     GQTNVPGIYAIGDITPGQALAHVASAEGIICVEKIAGHHPTPLDYNNIPGCTYCQPEI
                     ASVGYTEKGARDAGYEIRVGKFPFSASGKAKASGAPGGFVKVIFDAKYGEFLGAHMIG
                     ANVTEMIAEVVVARRLETTGMDIVKSVHPHPTMSEALMEAAAAAYGEVIHI"
     gene            complement(833..1303)
                     /locus_tag="PKM27_12895"
     CDS             complement(833..1303)
                     /locus_tag="PKM27_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNR08209.1"
                     /translation="MKDLQWLLDKYGESHTNKTNKLIHWICVPAIMFSLVGLLKSIPF
                     PVTRDFILNWAGFLLLGAMIYYLRLSVTMFLGFCLIAYLLLWGNHKIYLAMDRSYSGL
                     ALFSIILFVVAWIGQFIGHKIEGKKPSFLDDLQFLLIGPAWLMHFIFKKAGIKY"
     gene            1386..2138
                     /locus_tag="PKM27_12900"
     CDS             1386..2138
                     /locus_tag="PKM27_12900"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HNR08210.1"
                     /translation="MILFLTLLGFILSADSPIAVAAKKENRVFELRIYHCEPGKLNDL
                     IARFQNHTTSLFEKHGMTNIGYWVPMKEDNQSLYYVLAYPDLPSREASWKSFSADPTW
                     NEVRKKSEEQGKIVKSVESVFMNPEDFSPAIRPKASKKERVFELRIYTCHPGRLPALE
                     KRFRDHTLKLFKKHGMTNIAYWKSVEKDGQQPRLIYFLAHESEPAGKASFASFGKDPE
                     WIRVRDESERDGKIVQKLESIYMKALPFSAYR"
     gene            complement(2149..3099)
                     /locus_tag="PKM27_12905"
     CDS             complement(2149..3099)
                     /locus_tag="PKM27_12905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNR08211.1"
                     /translation="MIWIIFGLLLTGLLALDLGILNKGTQILTPHEATRNSIIWVSIG
                     LSFSIFVFFAYTHHWFGIGLEIGEAKAGGAALTEYLSGYLVELSLSVDNLFVFALVFR
                     YFNVPAQSQYRVLFWGILIALLLRGLMIFGGVLLVQRFFWIEYVFAVIIIYSAYRMWV
                     SGDDALNIEEMPLVIWLRKWMRMVHYYKEDHFFTKVSGHWHATPLFLTFLVVNIVDVI
                     FAFDSIPAIFAITRDEFLIFTSNIFAILGLRSMFFLLTGLLEKFVYLKTSLIVILLFI
                     GIKMLISHFYHIPSGFALLIILVILGTGIVTSINKKTEKE"
     gene            complement(3361..5436)
                     /locus_tag="PKM27_12910"
     CDS             complement(3361..5436)
                     /locus_tag="PKM27_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNR08212.1"
                     /translation="MMDGSSGTIKLKVEGMTCTGCAATIERYLEKSGLPEARVNHIDG
                     QVEFKASPSLDLQQVIRGIDRLGYKVIQAPEKQGRKLSIKQELIISLLFTLPLIFHHV
                     FPGLFHWLQSPLLQFLFALPAMSIGFRRFLPGAFRSVWAGNPNMDVTICLGALAALVY
                     SLLGWFLGQPDMIFFETAASILALLLLGNYMEHLAVKRTTQSIDELSKLRAEFGMMQM
                     PSGALVKIPVDELKRGDLLLVNEGDAVPTDAVILNGEGLVDESLITGESSPVPRKKGD
                     PLIGSSQVLAGNFIVKVTQTGEHTALGRIIELVKAASHAKAPIQKTADRISAVFVPLI
                     LAISLLSFLLNFLLLDVSLSQSVIRSIAVLVISCPCAMGLATPTAIVVGLGKAMRQGI
                     LIKGGSTLEALGKVKTVIFDKTGTLTHPEIKLQALKPGMEITIANALYAMESRSSHPL
                     ARAVTRFLSATYTLDPPAMTVKEVKGSGLEAVESNGVSWKIGWSPQHSKIEILREGEL
                     VAYLESRDSLKDQAVEHIRHLHNHHIRPVLLSGDSEEKVKEVAGQLGIEDWQARVMPE
                     QKAARIENYKKKGMTAMVGDGINDAPALSLADIGVSFAEASHAAVQSSQVVLLNDRLA
                     TFTKAMRIAKHTLAVIRQNLFWAFSYNIVAIPLAALGYLNPMWGVLFMAFSDLVVVGN
                     SLRLRWLRV"
     gene            5631..5706
                     /locus_tag="PKM27_12915"
     tRNA            5631..5706
                     /locus_tag="PKM27_12915"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5666..5668,aa:His,seq:gtg)
     gene            complement(5777..8614)
                     /locus_tag="PKM27_12920"
     CDS             complement(5777..8614)
                     /locus_tag="PKM27_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNR08213.1"
                     /translation="MKNLAVWIAVFSPLVMHAQISFSPATGLTSPAEHFSGSVMGISD
                     MDGDGLDDIVRLDQSKNLSIQYQSAPGRPFRSRDFGAVSGVEQWSLSIGDINQDGFND
                     MVFAANENPGTVFYSRKSGDSIVYDKQFLAESEKAYAQACNLVDINGDGWLDFFLCND
                     LGENRIWANNGSGRLDGSPVAWIDFTTVPASDKSGNYGSVWTDIDNDGDLDLYIAKCK
                     AGVDDPKDPRRVNALYVNKGNGTFFENASAAGLASGHQSWAVVAGDCDLDGDQDLFII
                     NHYAPCQLMINNGFGIFTEATGTGIQYDGAGVQAAWVDFDNDGMLDLLIAGSKHQLYL
                     NRGNHQFEAAPAQVFGPQQIESFVVGDLNHDGRMDIYAGYGLVFNKPSHRPDALWLNT
                     SPHANHYLKVRLEGLMPNKSAVGARISLHSGNYKIAREIHSGVSYGTSQSLTMHFGLG
                     SRTKVDSLVIRWPDKYREVIQEPFIDRILIVKQKACFAFDPDLKGGNLCTTGDSIVIT
                     APVNGNYEWSGGEKTRSLTVRKAGEYQLAMTTLEGCRIFSNRLYIGSGPIGEHRIQAS
                     DTVICAGTSASLRLETNLSVLWNTGDTSKTIQVSAPGPYFGRVSESCVPVQSDTVRLR
                     TIIPSEVIGRNDTIGPFAKATLRAEGESIVWYAEPSGGSPLAFGNTYFIQTPLDKTTT
                     FYAQSVLTLPGAKFNVGLKEHSGTTKFHANNFSGRLLFDVKQASVLKSVKVYADTPAL
                     RQIDLLNAAGEILASKLVRIEAGESRVILNFNLPPGDGYALAANEEVSIKQFGSRSPK
                     LYRTEGEVGFPLKGGPVSIYATNAGAGNYYYFYDWELAYQDLVCASPRVPVRAVILTV
                     ATRDQAVQPFRIFPNPANRFLNIEWKGPVPSREVRFIFSSIDGQILLSASAHPSGPNW
                     RIPLPQWDPGIYLLRVKSGENEFVSRFAVIN"
     gene            complement(8611..9147)
                     /locus_tag="PKM27_12925"
     CDS             complement(8611..9147)
                     /locus_tag="PKM27_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08214.1"
                     /translation="MMIQPAVLALLLHCGSCFSLCAQSYFTTGGIRLGSDYGISLKQL
                     ILKRTTLEMIVSTDDQAKTNLGLCLLAAQHKPILTRNLNIFYGAGLAWNWQVEDLPEG
                     LVKRTLFGIPVQAGLEFKVGRINFSWDYTPVLYISTKSNAFDSLKGLTVRYVFLNRKE
                     GKRLVKQLKSIITTRKGK"
     gene            9237..10073
                     /locus_tag="PKM27_12930"
     CDS             9237..10073
                     /locus_tag="PKM27_12930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR08215.1"
                     /translation="MNSGLSFRMKQWWSYYQRAKTIHQADSPFLYRFCREVLESDAKD
                     PWFEKIENLRGDLLKNNELFVHTDFGAGSGKPGKKQSMHRLSDFIRRSAIRPESGRLL
                     HRISRFFEANHILELGTAAGLSTAYLSKPGGKGQLITVEGDSYLASVAKATFDKLGLQ
                     PDLLIGAFDQVLPGILGHHPVFDLIFIDGNHRGAALESYIRLLSPYLHPDLGVIILDD
                     IHWSRDMAGAWDRLRLERQWNLSLDLFQFGLLIRNRDLLHFNTLDLIARKWKPLNPGI
                     FR"
     gene            complement(10070..12703)
                     /locus_tag="PKM27_12935"
     CDS             complement(10070..12703)
                     /locus_tag="PKM27_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR08216.1"
                     /translation="MEDYIQENREDRYKFKELKVYSSTEWLADQKFKYRQVFDRNETT
                     FIYVELSLYNKFFDKESWEAVIHLKCFSLSNNKQELCNLEFKKQISKYEPLVVLQEGW
                     GNKKEGAFWKKGTYYWEAWIFDEKVGSKYFYIEDAGEPMTRIHNPYFDIKSLRFYEGN
                     EDDISLDDRVYLKNFSAAETRLVHLELQLKNKNRSHHWQCELFFRFYNETRELKAQVV
                     KLKSIRKEEDLVIFAASCGMPSKGSWKKGRLAMELVFMDSLIASAVLDFREHAEPGNV
                     PVFLPNQSIPGFLNAEQDQDLSLEQLLGQLDQLVGLTQIKRQVQNHAKYLQFVQLRKQ
                     YGFREEDEINVHSVFIGNPGTGKTTVAHMMGNLYHKMGLLSKGHVHLVDRVDLVGEYI
                     GQTAPKVREAIEKARGGVLFIDEAYALARSNDDSKDFGREVIEILVKEMSNGPGDMVV
                     IVAGYPKEMKYFLDSNPGLKSRFKLYYEFADYTPEELEKIAQVACLKKEVSLSEAARV
                     KIYEFILEAYRNRDRAFGNARFVYDLIEKAKINLGLRIMKNPGKLPDEYEEGELAEIL
                     LEDVEKIVFQDAQKSAVIPVDEKLLTASLTELDSLIGMNAIKKQIHELVDIVRYNHRV
                     GKDVLRHFYLHTILIGNPGTGKTTVARILAKIYKALGILERGHMVETDRQGLVAGYVG
                     QSALKTSEKIDEAIGGVLFIDEAYSLLSTRTAGVDYGNEVIQTLLKRMEDQRGSFFVF
                     VAGYPDNMEDFIKANPGLSSRFDKTLRFDDYQPEELGEIAGKMISEHGLSISPEAMSY
                     LQDYLKFLFQYRDRYFGNARTVRSVILEAIQYHSLRIAKEGDKARPGSLHRIELVDLE
                     NFKMKTEDMHFQRKSIGFRNN"
     gene            12816..13157
                     /locus_tag="PKM27_12940"
     CDS             12816..13157
                     /locus_tag="PKM27_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNR08217.1"
                     /translation="MAKILFRFEDHRLEPVSVEQPAKGFSILEITEDHNIHLNHNCGG
                     VCACSTCHIYVDKGMEVLEELSEKEEDFIDRARNPKLESRLACQCIVLDENSEIEVTI
                     PDQSQIIGHEH"
     gene            13189..13437
                     /gene="iscX"
                     /locus_tag="PKM27_12945"
     CDS             13189..13437
                     /gene="iscX"
                     /locus_tag="PKM27_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNR08218.1"
                     /translation="MSIKNLEDLPIYWKDHEDIAMKLYERFGDEFDEGKIYRIRFTEL
                     IEWVLEIPHFSSPREECTEAHLEQIQAKWVYEWRDNNK"
     gene            13445..13939
                     /locus_tag="PKM27_12950"
     CDS             13445..13939
                     /locus_tag="PKM27_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HNR08219.1"
                     /translation="MPDTLLPLLHRIKSFVFDGVMTDGSLLLTEDGHSLRTFHIRDGY
                     AIRKALQEGYCLAVISGGRSEGIRHRLQGLGVTDLFLDAQEKEPVLRQWMADRSIEPD
                     ALAYMGDDILDLGAMAHAALRACPCDAVPEVKAIANYISPRPGGKACVRDLIELVLKV
                     QNRW"
     gene            13949..14896
                     /locus_tag="PKM27_12955"
     CDS             13949..14896
                     /locus_tag="PKM27_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNR08220.1"
                     /translation="MFSIASGFLRLIRLPNLVITALALWLHWFLLLRLPLNQAGIATA
                     FGHGSIACLGLVFALLMGAGFIINDIYDMQIDAVNKGDKRIVGNLIPVRAAWLAFYIL
                     LAVAAILALVIASYFNKTGFFWIFPVVALLLVIYSIWLKKTAFWGNLVIAILCGSVLL
                     IPLATESETLSSLSIPGRFLIFKNFFICAFTGGLLTLARELVKDIEDIPGDQAGQART
                     LPVLYGIGPSKAIAFLLTFTLVLLACFLMFKTENPFWRGALIILPGFILPLVYVLYRL
                     FKSTTPRDFHPIANLLKLCMVSGMTLLPLYLAQEHLWNG"
     gene            14884..15477
                     /locus_tag="PKM27_12960"
     CDS             14884..15477
                     /locus_tag="PKM27_12960"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNR08221.1"
                     /translation="MEWLKPYNIILASQSPRRLELMRQAGFQFEVRVADIDEENYPAD
                     LEITAVPGYLASQKAQKIFNQEPDQERLVVIAADSLVFLGGRIFSKPLNRPEAEWMLR
                     TLGGKTHLVITGLCVLSDSFSEIRSVETWVEFNPITDDEIHYYINHFPPYDKAGAYGI
                     QDWIGLCAVKGIQGSYTNIMGLPMETLYSVLKEKIKP"
     gene            complement(15478..15690)
                     /locus_tag="PKM27_12965"
     CDS             complement(15478..15690)
                     /locus_tag="PKM27_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08222.1"
                     /translation="MKKRSPNMLFVLALAISFVVFSYLNWCPSTSEALSGSPSSLLEV
                     SRGILRIPEFEMVMRFAEKVIDFVRP"
     gene            complement(15728..17158)
                     /locus_tag="PKM27_12970"
     CDS             complement(15728..17158)
                     /locus_tag="PKM27_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR08223.1"
                     /translation="MILLTAKRLNLSFDSPRGSIQVLRDIDLAVHEKESIGLTGPSGA
                     GKTQLAYTLGRLNEYFGGRIRCDALTYHRAGEEWNLMEDHRIRAFRKTEIGYIFQEPF
                     LYFNPTLRIKSQLALSSASGIKDENLMGKWLDRVGLKEQDRILNSFPHQLSGGQLQRL
                     AIAGCLLKNPRIIIADEITSSLDQGATQRILELIMQLKEELGFSILWVTHNETEVAKY
                     CDRIWSVKDGRLESDMPAAGFKARPFSLQRKKGGEPGTREILQVTGIRKKYGRAGVPG
                     FEVLRGIDLSLREGEITGLAGESGSGKSTLGRILAGMESWDQGRIHYNGRELNRGFKP
                     FREILYLFQDAFSSINPRITAQKLLDEALAAGERRMDQEFILKLTRLDQAILPRKAGE
                     LSGGLRQRFALARALAAMPRILIMDESVNALDVSLQEEILDMLLQYQIQYRLTILFIS
                     HQLEVIHRLCDRVVYLREGVLEEIRG"
     gene            17248..17979
                     /locus_tag="PKM27_12975"
     CDS             17248..17979
                     /locus_tag="PKM27_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNR08224.1"
                     /translation="MHRTLDGWHSPSLNKHMEIARYGHYGFALLMLPTAAADYLEYER
                     FHLIGAISHFIDHGKCKVFSVNSINSESWLNDHMHPRLKSIRHNQFNYYIYDEVIPYI
                     RTHTSWDTPIITCGASLGALHAANLFFRRPDLISGTIAMSGDYNLQSYTKGYYDDDVY
                     FNSPQAYLSNLNDEHYLSRIRSSRHIHILTGSGNWENPQASVHFSRVLHAKGIPHELD
                     VWGHDMPHDWPTWRAMLPHYLGTRF"
     gene            complement(18010..19098)
                     /locus_tag="PKM27_12980"
     CDS             complement(18010..19098)
                     /locus_tag="PKM27_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08225.1"
                     /translation="MRQVLLFLLLSLQLGLRAQSAYSNEFLTIGVGARAHGMGGAVVA
                     SVNDVNSAYWNPAGLSHIDAPLQLGAMHAEWFGGVGKFDHISLARRLNADRAAVGSIS
                     MVRLAIDQIPNTLNLVAPDGSIVFDNVSEFSSADYGFLISYATAIGEWDEKKSNRLGA
                     NVKVIHRQIGPFAKAWGFGIDLGMQWVRGPWQFGLVGQDLSTTFTGWRYSFTEEQKIQ
                     LEKTGNIIPVSALEKTLPHLGAGILRRFGKEDAFYCNVELGMNVFFDGKRNTVVASEF
                     ASLEPKAGLEAAYKKYVFLRGGYGNIQKIKDELDPLRTNTRGQLYSGVGVRFGRVQID
                     YALSNIGNLAKELNYSHIFSATLSFTGE"
     gene            19509..21221
                     /gene="ggt"
                     /locus_tag="PKM27_12985"
     CDS             19509..21221
                     /gene="ggt"
                     /locus_tag="PKM27_12985"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNR08226.1"
                     /translation="MNKRISGLWVLFLILLAQCAPKINPSGYWTGISKKAVSAKAMVV
                     SAHPLASKVGIRILHQGGNAVDAAVGVQFALAVVYPRAGNIGGGGFMVYRAASGETNT
                     LDYREKAPALASRDMYLDSLGNPGTGSVLGHLASGVPGTVDGLITAHAKYGKLSFEKI
                     IAPAIRLAEDGFQVTADEAARLQDYREVFVRTNGMENAFVKANKWKEGDWLIQKELAW
                     TLKQIRDRGRAGFYEGPVADRIVAEMIQGKGIISHDDLKAYRSVWRKPVIVPYRDYKV
                     ISMAPPSSGGIALGQLLKMVEPYPLKDWGFRDSRTIHLMVEAERRVYADRAQYLGDAD
                     FYPVPQDSLLNPKYLKYRMQDYREEQASKSSSVYAGNFKLALEHFETTHLSVVDQYGN
                     AVAVTTTLNGNFGSKVIVDGAGFLMNNEMDDFSVKPGVPNMFGLIGGEANAIQPGKRM
                     LSSMTPTIVEKNGKLSMVVGTPGGSTIITSVFQVILNVLEWNMPMGEAVAASRFHHQW
                     LPDEIILEENAFDPVLKSKLESMGHKIRSISSIGLVDAIRVRPDGGLEGGADPRGDDH
                     AEGW"
     gene            22428..22835
                     /locus_tag="PKM27_12990"
     CDS             22428..22835
                     /locus_tag="PKM27_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08227.1"
                     /translation="MTDEEKSKIMMEEEHKLMAGYMDTAKTFTQLSIGALVLSITFLE
                     KILGFKDKIPIHYLLLIAWLFWLLAALSGAFYQYRAVKWLEKLGIESNLIKKQNHVGD
                     LLKVYPYQVYGAFLVFFFLGTIFFAIFGATIIL"
     gene            23102..23527
                     /locus_tag="PKM27_12995"
     CDS             23102..23527
                     /locus_tag="PKM27_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08228.1"
                     /translation="MTSDDKKLWRERWLGCINELTSLDLQKKSWLDKTNINPHWSFVE
                     FMCSYFDDLGIDDNYKYAIDKGWLTDQEFEIIKDWHEALDKYDSPKNDDYDHAAILND
                     PTWLDILQIGLTMKSKLTPTLNETERKILTEEINYLNFV"
BASE COUNT         5875 a         6122 c         5988 g         5787 t
ORIGIN      
        1 gtcaccgtgg ttgaattcat ggagcaggga ttggtccccc gagaagatga agatatttca
       61 aaagagctct cccgcgcctt caataaatcc gggatccggg ttcatgccaa tacggaggtg
      121 acccgggtgg atacttccgg gcccggatgt ttggtttatc tcaaaaacag gaaaaccggg
      181 caggaagaaa gcctggcctg cgatgtggtc ttgtcggcgg ccgggatcac cccgaatctt
      241 gaaaacctcg gcctcgagac tgccggagtt caacttgaaa aagggctggt gaaggtggac
      301 ggttatggcc aaaccaatgt cccaggtatt tacgccatcg gagacatcac cccggggcaa
      361 gctctggccc atgtggccag cgcggaaggc atcatctgtg tggaaaaaat agcggggcac
      421 caccctacgc ctttggatta taataatatt ccaggctgta cctattgtca gccggaaatc
      481 gcttcggtgg gttatactga aaaaggtgcc cgggatgccg gttacgagat ccgggtgggt
      541 aaattcccat tttccgcatc gggtaaggcc aaagcatccg gcgctccggg cggctttgtc
      601 aaagtgattt ttgacgccaa atacggggag ttcctggggg cgcatatgat cggggccaat
      661 gtgacggaaa tgatcgctga agtcgtagtg gcgcgcaggc tggaaactac aggcatggat
      721 attgtcaaat ccgtgcaccc gcaccccacc atgagcgaag cgcttatgga agctgcggcg
      781 gccgcctacg gggaggtgat ccacatctga tcggctttca ttccccggcg cgtcaatatt
      841 ttatacccgc ctttttgaaa ataaaatgca tcagccaggc ggggccaatc aataaaaatt
      901 gcaggtcatc caggaaggag ggttttttgc cttcgatctt atggcctata aattggccga
      961 tccaggctac cacaaaaagg atgatggaaa atagggccag ccccgaatag gagcggtcca
     1021 tggccaggta aatcttgtga tttccccaga gcaggaggta ggcgatcagg cagaagccta
     1081 aaaacatggt taccgataaa cggagataat aaatcatggc ccccagcagc aaaaagcccg
     1141 cccagttgag aataaaatcc cgagtaaccg gaaagggaat ggatttgagc agtcccacca
     1201 ggctaaacat gatggcgggg acacaaatcc agtggatcag cttattggtc ttgttggtat
     1261 ggctttcccc gtatttgtcc agcagccatt gcaaatcttt catcggcaag gagtttgtga
     1321 tccacaagat aaccaatcgg agcgatttct ttgtagcttt agcgcccact taatccagaa
     1381 agaaaatgat attgtttctt acgcttttag gctttattct cagcgctgat tcacccattg
     1441 ccgttgctgc aaaaaaagag aaccgggtct ttgaactcag gatttaccat tgcgaaccgg
     1501 gtaagctgaa tgacctgatt gcccgtttcc aaaaccatac caccagcctt tttgaaaaac
     1561 acgggatgac caatatcggt tactgggtgc ccatgaaaga agacaaccaa agtttgtatt
     1621 atgttttggc ctacccggac ctgccctccc gggaagccag ttggaaatcg ttttccgcgg
     1681 accctacctg gaacgaagtg cgcaagaaat ccgaggaaca gggcaaaatc gtaaagtccg
     1741 tggaatcggt gtttatgaac ccggaggatt tttcccccgc cattcgcccc aaagcctcaa
     1801 aaaaagaaag ggtgtttgag ctgagaatat atacctgcca tcccggacgc cttcccgccc
     1861 ttgaaaaacg cttccgcgac cataccctga aactttttaa gaaacacggc atgaccaata
     1921 ttgcctattg gaaaagcgtg gaaaaggatg gccagcaacc caggctgatc tatttcctgg
     1981 ctcatgaaag tgaaccggcg ggcaaggctt cttttgccag ttttggcaag gacccggaat
     2041 ggatccgggt aagggacgag tcggaaaggg acgggaagat tgtgcagaag ctggaatcca
     2101 tctatatgaa ggccctcccc ttctctgcct accgctgatc cggcagcctt attccttttc
     2161 cgttttttta ttgatcgaag tgacgatccc ggtcccgaga attacaagga tgatgagcag
     2221 ggcgaagccg ctgggaatat ggtaaaaatg gctgatcagc atttttatcc cgataaagag
     2281 gaggatgacg atcagggaag tcttgagata aacaaatttc tccaacaaac cggtgagcag
     2341 gaaaaacatg ctcctcaggc ccagaatggc aaagatattg gaagtgaaga tcaggaactc
     2401 gtcccgcgtg atggcgaaga tggccggtat ggaatcaaag gcaaaaatga cgtccacgat
     2461 attgaccacc aggaaggtca ggaacagggg cgtggcgtgc caatgaccgg aaaccttggt
     2521 aaaaaaatgg tcttccttgt aataatgcac catgcgcatc catttgcgca gccagatcac
     2581 caggggcatt tcttcgatgt tcagcgcatc gtccccggat acccacatgc ggtaagccga
     2641 ataaatgatg atcacggcga atacatactc tatccagaaa aaccgctgaa ccagcaggac
     2701 gccgccaaaa atcatcaggc cccttaacag gagggcgatc aggatccccc aaaaaaggac
     2761 ccggtactgg gattgggcag gcacattgaa gtaccggaaa accagggcaa acacaaataa
     2821 attgtccaca ctcagggaca attccaccag gtaaccggaa agatattcgg tgagggccgc
     2881 tccccccgcc tttgcctcac caatttcgag tccgatgcca aaccagtgat gggtataggc
     2941 gaaaaaaacg aaaatgctga aagaaagccc tatcgaaacc cagatgatgg aattccgggt
     3001 cgcttcatgc ggggtgagga tctgcgtgcc tttgttcaat atacccaaat ccaatgcgag
     3061 caagccggtc aacagcaagc caaagatgat ccatatcata aatgaggcgc caaagttaaa
     3121 aattttgaag gctgtgcatt ggaattaaac ccaaatcatg caatagagtt tcgtaatgaa
     3181 agccggaagg ccaataaaga ctggcgctcc cggactattt ttaccgcaaa cataggcact
     3241 cctatggtga ggataaaaat gggaggccga cctaaatcat aaatcaaact attcggccgg
     3301 gaagtgtcag tatttgcagg cattccggct tgtaatagaa aatctgttgc atggtttggg
     3361 ttaaaccctg agccacctga gccggagcga attgcctacc accaccaggt cggaaaaggc
     3421 catgaacagg accccccaca tgggattgag ataacccagg gccgccaggg gaatggccac
     3481 gatattatat gaaaaggccc agaagaggtt ttgcctgatg acggccaggg tgtgtttggc
     3541 tatgcgcatg gcttttgtaa acgtagccaa ccgatcgttt agcaggacca cctgggagga
     3601 ttgtacggcc gcatggcttg cctccgcaaa ggataccccg atatcggcga ggctcagggc
     3661 aggggcatcg ttgatgccgt ccccgaccat ggccgtcatg ccttttttct tataattttc
     3721 aatccgggcg gccttctgtt ccggcataac cctggcctgc cagtcctcga tgcccagctg
     3781 gccggccact tccttcacct tttcttcact gtccccggac aacaaaaccg gccggatgtg
     3841 gtggttatgc aagtgccgga tgtgttccac ggcctggtcc ttgagactgt ccctggattc
     3901 aaggtatgcc accagttccc cctcccgaag gatttctatc ttcgaatgtt gcggagacca
     3961 cccgattttc catgaaaccc cattactttc gacggcttcg aggcctgagc ctttgacttc
     4021 tttaacggtc atagcgggcg gatcgagcgt ataagttgcg gaaaggaaac gggtcacggc
     4081 acgggctaaa ggatgtgaag acctggattc catggcatag agggcattgg caatcgtgat
     4141 ttccatgccg ggttttaagg cttgtagttt gatttccgga tgggtaaggg tacctgtttt
     4201 gtcgaaaatg acggtcttga ctttacccag ggcttccagg gtggaaccgc ctttgatcaa
     4261 aatgccctgc cgcatagcct tgcccagtcc gacaacgatc gcggtaggcg tagccaatcc
     4321 catggcgcat ggacaggata tcaccaggac ggcgatggac cggatgacgg actggctgag
     4381 cgaaacatca agtaataaaa aattcaataa gaaagacaga aggctgatgg ccaggatcag
     4441 gggtacaaag accgcgctga tgcggtcggc ggttttttgt atgggtgctt tggcatggct
     4501 ggcggcttta accaattcga tgattcgacc cagggccgtg tgttctccgg tctgggtcac
     4561 ttttacaata aaatttccgg caaggacctg gctggatcca atcaaaggat cccccttttt
     4621 cctcggcacg ggactgcttt ccccggtgat cagggattcg tccactagtc cttccccgtt
     4681 gaggattacg gcatccgtcg ggacggcatc cccttcattg acaagcaata agtcacctct
     4741 tttaagttcg tcgacgggga ttttcaccag ggcaccggag ggcatttgca tcattccaaa
     4801 ttccgcccgg agtttactga gctcatcgat agactgggtc gttcttttga cggccaggtg
     4861 ctccatataa tttcccagta aaagaagggc cagaatgctg gctgccgtct cgaaaaagat
     4921 catgtccggc tggcccagaa accagcccag caggctgtaa accagggcgg ccagggcacc
     4981 caggcagatg gttacatcca tattgggatt gccggcccag accgaacgga acgcaccggg
     5041 cagaaagcgt ctgaagccga tagacatggc cggcaaggca aaaagaaatt gtaataaggg
     5101 cgattggagc cagtggaaaa gacctgggaa tacatggtga aagatcagcg ggagggtaaa
     5161 aagcaggctg atgatcagtt cctgctttat ggaaagtttt cttccctgct tttccggcgc
     5221 ctggatcact ttatatccca ggcggtcaat gccccggatc acttgttgga gatccaggga
     5281 gggcgaggct ttgaattcca cctgcccgtc gatgtggttg acccttgcct ccggcaggcc
     5341 gcttttctca agatatcttt caatcgtagc cgcgcacccg gtacaggtca tcccctccac
     5401 tttcagtttt atcgtgccgg aagatccgtc catcattgtt taaacaaaca ggcaatataa
     5461 aaagttatcc ctcatcagga ggccggataa aactaacggt tttcttccat ctccttcttc
     5521 aacccataaa aagtactttt aaaaatacca gggattcccc cagccgacat ttgagataaa
     5581 aacagaaaat ggtatctttg cagcgctttt gaccaaaaca aaagtgaact ggtgaatgta
     5641 gctcagctgg ttagagcatc agattgtggt tctgagggtc gcgggttcga atcccgtctt
     5701 tcacccctcc aaggcccggg ttaaaaagat ccgggctttt tttttgaatc aaacaggagg
     5761 tatccggcgg atccttttag tttataaccg cgaacctgga cacaaattca ttttcaccgg
     5821 atttgacccg cagcaggtag atgcccggat cccattgcgg caaggggatc ctccagtttg
     5881 gccctgaggg gtgggcgctt gcagaaagga ggatttggcc atctatgctg ctgaatataa
     5941 atcgcacctc ccgggaaggc acaggaccct tccactctat attcaggaag cggtttgccg
     6001 gattgggaaa tatcctgaat ggttgaaccg cctgatcccg ggtggcaacc gtgaggatca
     6061 ccgcgcgcac cggcaccctg ggactagcac aaaccaggtc ctggtaggcc agttcccaat
     6121 cgtagaaata ataataattg cccgccccgg cattggtcgc gtaaatagat accgggccgc
     6181 ctttaagggg gaagcccacc tctccttccg tgcggtataa tttcggggac ctggagccga
     6241 attgttttat cgaaacctcc tcattggcag ccagggcata accgtccccc ggcggcaggt
     6301 tgaaattgag gattacccgg ctttccccgg cttcaatccg aaccagttta ctcgcgagga
     6361 tctcccccgc ggcgttcaat aaatctatct ggcgaagggc cggggtgtcc gcataaactt
     6421 ttacggattt caggacgctg gcttgcttca catcaaacag cagcctgccg ctgaaattgt
     6481 ttgcatgaaa tttcgtggtc cccgagtgct ccttaagtcc aacgttgaat ttcgccccgg
     6541 gaagggtcag caccgactgg gcgtaaaaag tggtggtctt gtcgagtggg gtttgaatga
     6601 aataggtatt tccaaaggcc agcggggaac cgccggacgg ttcggcatac catacgatgg
     6661 attccccttc agctcttagt gtggctttgg cgaaaggccc aatggtatcg tttcgtccga
     6721 tgacctcgga aggaatgatg gttcgaagcc tcaccgtatc cgattgaacg gggacacagc
     6781 tttcactcac ccgcccgaaa tagggaccgg gtgcggagac ctggattgtt tttgaagtgt
     6841 ccccggtatt ccagagcacg gaaaggttgg tttccaaccg taaactggcg gaggtcccgg
     6901 cacagatcac cgtatcggaa gcctggatgc ggtgttcccc tatcgggccc gacccgatgt
     6961 ataaacgatt ggaaaaaatc cggcatcctt caagcgtggt catggccaac tggtattctc
     7021 ccgctttgcg cacagtgagc gatctcgttt tttctccccc tgaccactca tagttgccgt
     7081 tgacgggagc ggtgatcacg atgctgtccc ccgtggtaca gaggttcccg cctttgaggt
     7141 cggggtcgaa ggcaaagcat gctttttgtt ttacaatcag gatccggtcg ataaaaggtt
     7201 cctggatcac ttcgcggtat ttgtcgggcc accttatgac gagggaatcg actttggtgc
     7261 ggctgcccaa accaaaatgc atcgtcaagg attgactcgt cccataactc accccggaat
     7321 gaatttccct ggcaattttg taattgccgg aatgaaggct tatccgggcc cctaccgcac
     7381 ttttattcgg catcaaacct tccaggcgca ctttcaaata atggtttgca tgaggactgg
     7441 tattcagcca taaggcatcc ggccggtgag aaggcttgtt aaaaaccaaa ccataacctg
     7501 catagatatc catacgtccg tcgtggttga ggtccccgac cacaaaagat tctatttgtt
     7561 gcgggccgaa gacctgtgcg ggggccgctt caaattgatg gtttccgcga ttgagataca
     7621 actggtgctt gcttcccgca atgaggagat caagcatgcc gtcattgtca aaatccaccc
     7681 aggcggcctg caccccggct ccatcgtatt ggatcccggt gcccgtagct tcggtgaaaa
     7741 tgccaaaacc gttgttgatc atgagctggc agggcgcata atgattgatg ataaacaggt
     7801 cctggtcccc gtccaggtca cagtctcccg ccactacggc ccaggactga tgcccggagg
     7861 ccaggccggc agcggaagcg ttttcaaaga aggtcccgtt acctttattg acatacaagg
     7921 cattcacccg cctcgggtct ttcggatcgt ccacccctgc tttgcatttt gctatataaa
     7981 ggtcaagatc cccgtcgttg tcgatgtccg tccacacgct tccgtaattg ccggatttat
     8041 cggaggcggg gaccgtagta aagtcaatcc aggctaccgg cgatccgtca agcctgcccg
     8101 atccgttgtt ggcccagatg cggttttcgc ccaggtcgtt gcaaagaaaa aaatcaagcc
     8161 agccgtctcc gttgatgtcc actaggttgc aggcctgcgc gtaggctttt tcggactcgg
     8221 ccaggaattg tttgtcataa acgatggaat cccccgattt tctcgaataa aatacggttc
     8281 cgggattttc attggccgca aagaccatat cgttgaatcc atcctggttg atgtccccga
     8341 tcgaaaggct ccattgttcc accccgctta cggctccaaa atcgcgggag cggaacggac
     8401 ggccgggagc ggactggtat tggatggaga gatttttgga ttgatccagg cgtacgatat
     8461 catcgaggcc atccccgtcc atatctgaaa tgcccatgac cgagccgcta aaatgttctg
     8521 ccggggaggt cagcccggtg gccgggctaa aactgatctg cgcatgcatc accaacgggg
     8581 aaaagactgc aatccaaacc gccaaattct tcatttacct tttcgggttg taatgattga
     8641 ttttaattgt tttaccaagc gtttcccctc cttcctgttt aaaaaaacat accggacggt
     8701 gaggccttta aggctgtcaa acgcattaga ttttgtgcta atatataaaa ccggggtgta
     8761 atcccaactg aaatttattc tccctacctt aaactccagt ccggcctgga ccgggatccc
     8821 aaaaagagtc cttttaacca ggccttcggg caggtcttcc acctgccagt tccaggccag
     8881 tccggctcca tagaatatat tgaggttccg ggtcaggatg ggtttatgct gggcagcaag
     8941 caggcatagg cccaggttgg ttttggcctg gtcatcggtg gacacgatca tttccaaagt
     9001 ggtccttttc aggatgagtt gtttgaggga gatcccgtag tcggagccca gccggatgcc
     9061 gccggtggtg aaataactct gggcgcaaag ggaaaaacac gagccgcaat gcaaaagcag
     9121 ggccaggaca gcaggttgta tcatcatgga attttgtaac cattgacgca ggaaaagaaa
     9181 tactattgcg aagagaaact taaaattgtg tctggttcta ttaatatttt aaacggatga
     9241 acagcggtct ctctttccgc atgaaacaat ggtggtctta ctaccagcgg gccaaaacca
     9301 tccaccaggc cgactcccct tttctttacc ggttttgcag agaagtcctc gaatccgacg
     9361 caaaggatcc ctggtttgaa aaaatcgaaa accttagggg cgacctgctg aaaaataatg
     9421 aactattcgt acacaccgat tttggtgcgg gatccggaaa gccgggcaaa aaacaatcca
     9481 tgcaccggct aagtgatttt atccgccgct ccgccatccg gccagaatct gggaggctcc
     9541 tacatcgcat cagccggttt tttgaagcca accatattct tgaactgggc accgcagccg
     9601 gactatccac cgcctacctc agcaagcctg ggggtaaagg tcaactcatt actgtcgaag
     9661 gagactccta cctggcctcg gttgcaaaag ccacttttga taagctagga cttcagccag
     9721 acctcctcat cggggcattt gaccaggtac tgcctggaat cctcggtcat cacccggtat
     9781 ttgacctgat cttcattgac ggaaaccacc ggggcgcggc cctggaatcc tatatccggc
     9841 tactgagtcc atacctccat cctgatctgg gagtgatcat cctggacgat attcactgga
     9901 gccgcgatat ggccggggcc tgggacaggc tccggcttga aaggcaatgg aacctcagtc
     9961 tcgatttatt tcaatttgga ctgttaatca ggaaccggga tctccttcac ttcaatactc
    10021 ttgatttgat tgcgcgaaaa tggaagcctc ttaatcccgg aattttccgc taattatttc
    10081 tgaagccaat ggattttctt tgaaaatgca tgtcttcggt cttcatctta aagttttcga
    10141 ggtctaccag ttcaatccgg tgcaggctgc ccggcctggc cttgtcccct tctttggcga
    10201 tccggaggct atggtactgg atggcttcca ggatgactga gcgaaccgtc ctggcattac
    10261 cgaaataacg gtcccggtac tggaagagaa atttaaggta atcctgcaga tagctcatgg
    10321 cttccggact gatgctgagc ccgtgttccg agatcatttt accggcaatt tccccgagtt
    10381 cttcaggctg gtagtcgtca aaacgaagcg ttttgtcaaa ccttgaactg aggccggggt
    10441 tggctttgat aaaatcttcc atattgtccg gataacccgc gacaaataca aaaaaactgc
    10501 cccgctggtc ctccattctt ttgagcaggg tctgaatgac ttcattcccg taatccaccc
    10561 cggccgtgcg ggtggacagg agggaatatg cttcgtcaat gaacagcacc cccccgatgg
    10621 cttcatcaat cttttccgag gtcttgagtg cggactggcc cacatatccc gcaaccagcc
    10681 cctggcggtc ggtttccacc atatggccgc gctcgaggat gccgagggct ttgtagattt
    10741 tggccaggat cctggccacg gtggttttgc ccgtacccgg attgccgatc agtatggtgt
    10801 gcaaataaaa atggcgcagg acgtctttgc cgacccggtg gttgtagcgg acgatgtcca
    10861 ccaactcatg gatttgtttt ttgatggcat tcattccgat caggctgtcc aattccgtca
    10921 gcgaagcggt caacaatttt tcatctaccg ggatgacggc ggatttctgg gcatcctgga
    10981 aaacaatttt ctccacatct tcgagcagga tttccgccaa ttcgccttcc tcatattcgt
    11041 ccggcaattt gcccgggttc ttcatgatcc ggaggcccag gttgatcttg gctttttcga
    11101 tcaggtcata gacgaaacgg gcgttgccga aagcccggtc ccggttgcgg taagcctcca
    11161 aaataaattc atagattttt acccgtgcag cctccgacag gctcacctcc tttttaagac
    11221 aggccacctg tgcgatcttt tccagttcct cgggagtgta atcggcaaat tcataataca
    11281 atttaaacct ggatttgagc cccggattgg agtccaggaa atatttcatc tccttggggt
    11341 atcccgccac gatcacgacc atgtcgcccg gcccgttgga catttccttg accagtattt
    11401 cgatgacctc ccgcccaaaa tccttgctgt cgtcgttgga ccgggccagg gcgtaggctt
    11461 cgtctataaa caaaacccct cccctcgcct tttctatggc ctccctcact ttgggcgcgg
    11521 tctgcccgat gtattcaccc accaggtcaa cccggtcgac caggtgcaca tgccctttgg
    11581 acaagaggcc cattttatgg tacaggttgc ccatcatgtg ggctacggtg gtctttccgg
    11641 tgcccggatt accgatgaaa acggaatgga cattgatctc atcctcttcc cggaaaccat
    11701 attgtttccg gagctggaca aactgaagat atttggcgtg gttctgcacc tgcctcttga
    11761 tctgggtaag gcctaccaac tgatccaact gccccaggag ttgctcaagg ctcagatcct
    11821 ggtcctgctc cgcattgagg aaaccgggaa tggactggtt gggcaggaaa acagggacat
    11881 tcccgggttc agcatgttcg cggaagtcca ggacggcgga agcgatgagg gaatccatga
    11941 acaccagttc catcgccagc cttccttttt tccagctgcc tttggaaggc atgccgcagg
    12001 aagcggcaaa gatcaccagg tcctcttctt tccggatgct tttgagcttg acgacctgcg
    12061 ctttgagttc tcgggtttcg ttgtaaaaac ggaaaaacaa ttcgcattgc cagtggtggg
    12121 acctgttttt gtttttcagc tgcagttcaa gatgcaccag ccgggtttcg gccgcggaga
    12181 agtttttaag ataaacccgg tcgtcgagac tgatgtcgtc ttcgttgcct tcataaaaac
    12241 gcagcgattt gatgtcaaaa taggggttgt ggatcctggt catgggctcg ccggcatctt
    12301 cgatatagaa atatttgctg cccacctttt catcgaagat ccaggcttcc caatagtagg
    12361 tgcctttttt ccagaaggcg ccttcttttt tattgcccca gccttcctgg aggaccacca
    12421 ggggttcgta tttgctgatc tgttttttaa actccaggtt gcacaattcc tgtttgttgt
    12481 tggacaagga aaaacatttg aggtggatga cggcctccca gctttccttg tcaaaaaatt
    12541 tattgtaaag ggacagttcc acatagatga aggtggtttc gttccggtcg aacacctgcc
    12601 tgtatttgaa cttctgatcg gccagccact ctgtggaact gtataccttc agttctttga
    12661 atttataacg gtcttcccgg ttttcctgaa tataatcttc catgaatatt gttcagtcaa
    12721 attgcgtttt ttcagccaat attccaaaaa attatttttt ctaaacttat gcccttatcc
    12781 atacgttaaa gcatttgatt tacttttaca cccgaatggc aaaaatcctg tttcgttttg
    12841 aagaccatcg gttggagccc gtttcggtgg aacaacccgc caagggattc agcatcctgg
    12901 agatcacgga agaccacaac attcacctca atcacaattg cggcggcgtc tgcgcctgca
    12961 gcacctgtca tatttatgtg gacaagggca tggaggtgct ggaggaactt tcggaaaagg
    13021 aagaggattt tattgaccgc gcccggaatc cgaagctcga atcccgcctg gcctgccagt
    13081 gcatcgtgct ggacgaaaat tccgagatcg aggtcaccat tcccgaccag tcccagatca
    13141 tcggccacga acactgatcc tttgttcaac ccgccaaaag caaattcaat gtcaatcaaa
    13201 aacctggaag acctgcccat ttactggaaa gaccacgaag acatcgccat gaaactgtac
    13261 gagcggtttg gcgatgaatt tgatgagggc aaaatatacc ggatccgctt tacggaactc
    13321 attgaatggg tgctggaaat cccacatttt tccagtccca gggaagaatg tacggaagcc
    13381 cacctggagc agatccaggc taaatgggtc tatgaatggc gggacaacaa taaataatcc
    13441 cggcatgccc gataccctgc tccccctcct gcaccgcatc aagtcctttg tattcgacgg
    13501 ggtcatgacg gacggaagcc tgctcctgac cgaggacggc cacagcctac ggacttttca
    13561 cattcgcgac ggatatgcca tccgcaaggc ccttcaggaa ggatattgcc tggcggtgat
    13621 cagcggaggt cgttcggaag gtatcaggca ccggttgcag ggcctggggg tcacagacct
    13681 ttttctcgat gcccaggaaa aggaacctgt gcttcgacaa tggatggcgg accggagcat
    13741 agaacccgac gccctcgcct atatgggtga cgatatcctg gacctcgggg caatggccca
    13801 tgccgccctg cgcgcctgcc cctgcgacgc ggtaccggaa gtcaaggcca tcgccaatta
    13861 tatctctccc cggcccggag gcaaagcctg cgtcagggac ctcatcgaac tcgtgctaaa
    13921 agttcagaat cggtggtaat ttcaagccgt gttttccatt gcctccggtt ttcttcgact
    13981 gattagactg cccaacctgg tcatcaccgc gctggcgctc tggttgcatt ggttcctttt
    14041 gttgcggctg cctttgaatc aggccggaat agcgaccgcc ttcgggcatg gaagcattgc
    14101 ctgcctcggg ttggtcttcg ccctgcttat gggcgccgga ttcatcatca acgatatata
    14161 cgacatgcag attgacgccg tcaacaaggg ggacaaacga attgtaggga acctgatccc
    14221 cgtaagggcg gcctggctgg ctttttatat cctgctggct gtggcggcta tcctcgccct
    14281 ggtcatcgcc tcctatttta ataaaaccgg tttcttctgg atcttccccg tggtcgccct
    14341 gctcctggtg atctattcca tctggctgaa aaaaacggcg ttttggggca acctggtcat
    14401 cgccatcctt tgcggatcgg tattgttgat tccactggcc accgaatcgg aaaccctctc
    14461 ctccctttcc attcccggcc ggttcctgat ctttaagaat ttttttattt gcgcttttac
    14521 cggcggcctg ctcaccctgg ccagggaact ggtcaaagac attgaagaca tccccgggga
    14581 ccaggccggc caggcccgca ccctgcctgt gctttacggc attggccctt caaaagccat
    14641 cgcattcctc ctgaccttta ccctagtcct gctcgcctgc ttccttatgt ttaaaacgga
    14701 aaatccattc tggcgcggag ccctgatcat ccttcccggc tttatcctgc ccctggtgta
    14761 tgtgctttac cgcttgttca aatccactac cccccgtgat tttcatccta ttgccaacct
    14821 cttgaaacta tgcatggtca gcggcatgac cctgctccct ttatatcttg cccaggagca
    14881 cttatggaat ggttgaaacc ctataatatc attttggctt cccaatcacc ccgtcgcctg
    14941 gaactgatga ggcaggccgg cttccagttc gaggtaaggg tggccgatat cgatgaggaa
    15001 aattatcccg ccgaccttga aatcaccgcc gtacccgggt acctggccag ccaaaaagct
    15061 caaaagatat tcaaccagga accggatcag gaaagattgg tcgtgatcgc ggccgacagc
    15121 ctggtattcc tgggtggacg gatcttttcc aaaccactga accggccgga agcggaatgg
    15181 atgctccgga cccttggggg caaaacccac ctggtcatta ccggtctttg tgtactttcc
    15241 gactccttct ccgaaatccg ttccgtggaa acctgggttg aatttaatcc catcaccgac
    15301 gatgaaattc attattatat caatcacttc cctccttatg acaaagccgg cgcctatggc
    15361 atccaggact ggatcggcct ctgcgccgta aaagggatac agggatccta taccaatatc
    15421 atggggctgc ccatggaaac tttatattcg gtactgaagg aaaaaatcaa accctgatca
    15481 ggggcggaca aaatcgatca ccttttccgc aaaacgcatg accatctcaa attcagggat
    15541 acgcaagatg ccgcgggata cttccaaaag actggaagga gaaccgctca gggcctctga
    15601 agtgcttggg caccagttga gataggaaaa aaccacaaaa ctgattgcaa gcgctaatac
    15661 aaaaagcata ttgggagaac gctttttcat ttcgcaaaaa taagggagtg aagtcagaaa
    15721 atcaagctta tccccttatt tcttccagaa ccccttccct gaggtagact acccggtcgc
    15781 agaggcgatg gatgacttcg agctgatggg aaataaaaag gatggtcaac cggtattgaa
    15841 tttggtactg caaaagcata tccaaaatct cttcctggag gctgacgtcc agggcgttga
    15901 ccgattcgtc catgatcagg atccggggca tggccgccag ggcccgggcc agtgcaaagc
    15961 gttgcctgag gccacctgaa agttcgccag cttttctggg caatatggcc tggtccagcc
    16021 tggtaagctt tagtatgaat tcctggtcca tccggcgctc cccggccgcc agggcctcat
    16081 caagcagctt ttgagccgtg atcctgggat tgatcgagct gaaggcgtcc tggaacaaat
    16141 aaaggatttc acggaagggt ttaaaccctc tgttgagttc gcggccatta taatggatcc
    16201 ggccctgatc ccaggattcc attcccgcca ggatacggcc aagggtggac ttgccggatc
    16261 ccgattctcc ggccagtccg gtgatctccc cttcccggag acttaggtca atgcccctga
    16321 ggacttcaaa accagggacg ccggcccggc catatttttt ccggatcccg gtgacctgca
    16381 ggatttcacg ggtgccgggc tccccgcctt ttttccgctg aaggctgaat ggacgggctt
    16441 tgaatcccgc agcaggcata tcggactcca gccgcccgtc ctttaccgac cagatgcggt
    16501 cgcaatattt ggcgacttcc gtttcattgt gggtcaccca aaggatactg aaacccagtt
    16561 cttccttaag ttgcataatc agttccaaaa tcctttgcgt agcgccctgg tccagggatg
    16621 aggtgatctc atcggcaatg atgatccttg gatttttcaa aaggcagccg gcaatagcca
    16681 gccgttgcaa ttggccgccg gagagctggt gaggaaaact gttcaggatg cggtcctgtt
    16741 ccttcaagcc cacccggtcc agccatttgc ccatcaggtt ctcgtctttt attccggaag
    16801 cggaggaaag agccagttgg cttttaatcc gcagggttgg gttaaaataa agaaacggct
    16861 cctggaatat ataaccgatc tcggttttac ggaaagccct gatccggtgg tcttccatca
    16921 ggttccattc ctcacccgcc cggtggtagg tcaaagcatc gcaacgaatg cggccgccaa
    16981 aatattcgtt cagccttccc agggtgtatg ccaattgggt tttccccgcg ccgctcggcc
    17041 cggtgaggcc gatgctctct ttttcatgca cggccaggtc aatatccctg agcacctgga
    17101 tggatccccg gggcgaatca aaggaaaggt tcagccgttt ggcggtcagc aaaatcatcc
    17161 ccgggcaaga taatcatagg acccgaggat ttcattcctg aataatattg gatatttaca
    17221 gcctgaatcc aaattcaacg atcaaacatg caccggaccc tggatggatg gcatagccct
    17281 tctttgaaca aacacatgga aattgccaga tatgggcatt atggctttgc tttactgatg
    17341 ttgcccaccg cggcggccga ttacctcgaa tacgaaaggt ttcatctcat cggggccatc
    17401 tcgcatttta tcgaccatgg caagtgcaag gtgttttccg tcaacagcat caattcggaa
    17461 agctggctca acgatcacat gcatccgaga ctgaaatcca tcaggcacaa ccagttcaac
    17521 tattatatct acgatgaggt gatcccctat atccggaccc atacctcctg ggatactccc
    17581 atcatcacct gcggggcttc cttgggcgcc ctgcacgcgg ccaacctttt tttccggcgg
    17641 cccgacctga tctccggtac cattgccatg agcggggact ataacctgca atcctatacc
    17701 aaaggatatt atgatgacga tgtctatttt aattcccccc aggcctacct gtccaacctg
    17761 aacgatgagc attacctttc ccggatccgg tcgagcaggc atattcatat actgaccggc
    17821 tcaggcaact gggaaaatcc gcaggcctcc gtacattttt ccagggtcct gcacgcgaag
    17881 ggcatcccgc acgaactcga tgtctggggc catgacatgc cgcacgattg gccgacctgg
    17941 agggccatgc tcccccatta cctgggtacc cgcttttaat ggaagataat caccgggcaa
    18001 gtccggcctt cactcacccg taaaactcag ggtggcggaa aaaatgtggg aataattcaa
    18061 ttctttggcg aggttgccga tgttggatag ggcgtaatca atctggaccc tgccgaagcg
    18121 gacgcccacc ccgctgtaca attggccacg cgtatttgtc cggaggggat ccagttcgtc
    18181 ctttattttt tgaatatttc catacccgcc gcggaggaat acatattttt tatacgccgc
    18241 ctccaggccg gccttaggtt ccaggctggc aaattccgaa gccaccacgg tatttctttt
    18301 gccatcgaaa aagacgttca ttcccagttc gacattgcaa taaaaggcat cctcctttcc
    18361 aaagcgccgc aggataccgg cgcccaggtg gggcagggtt ttttccaggg cgctcaccgg
    18421 gatgatattg ccggtttttt ccaattggat tttctgttct tctgtaaaac tatatctcca
    18481 gccggtgaaa gtggtgctga ggtcctgccc gaccagccca aactgccagg ggccccggac
    18541 ccactgcatc cccaggtcaa tgccaaaacc ccaggccttg gcaaagggtc cgatctgccg
    18601 gtggatcact ttgacgtttg cgccgaggcg gtttgatttt ttttcatccc attccccgat
    18661 cgcggtggcg taagagatga ggaatccgta gtccgcgctg gaaaattcac taacattatc
    18721 aaaaacaatg cttccatcag gagccaccag gttgagggtg ttggggatct ggtcgatggc
    18781 cagccggacc atactgatgc ttcctacggc ggcccggtcc gcattgagcc tccgggccag
    18841 ggaaatatga tcaaatttgc ccacccctcc gaaccattcc gcatgcatgg cgccgagttg
    18901 caggggtgca tcgatatgag aaaggccggc cggattccaa taggcggagt tgacatcgtt
    18961 taccgaggcg accaccgcac cccccattcc atgagccctg gcgcctacgc ctatggtcag
    19021 gaattcattg gaatatgccg attgggcacg gagtccgagc tgcaaagaca gtaggaggaa
    19081 aagcaagacc tgcctcattg atcaaagata atgaaataga tatgttggga cgtagcttgg
    19141 ctgcactgtt tggacgaagc ttagcttcgg ccttgcatgc actgtttgga cgaagcttag
    19201 cttcggcctt gtattcgagg ttgctccagg gcgtcctcgc ctggagaggt ttaacttaaa
    19261 tgggcgggag aatggattat gttatccctc attttgcaaa cagcacctta ttgcaggctt
    19321 tgggttaata tttttgcgaa cgttattttt gattgcctgc ccctgacccc tccagggaac
    19381 ctaccacctg gctgttggaa ttaacccaaa ggtatggccc tgcgcttatt aagaattttt
    19441 cagactgtga ttccatgagt cgtatcacca accccttatt atctttgaat ccatcaaatg
    19501 aaaaccttat gaataaaaga atctccggcc tctgggtctt gtttctgatc ctcctggccc
    19561 aatgcgcgcc gaagatcaat ccttcgggct actggaccgg gatctccaaa aaagccgtct
    19621 cggcgaaagc catggtggtc agtgcccatc ccctggcttc aaaagttgga atccgcatcc
    19681 tgcaccaggg gggcaatgcg gtggatgctg cggtgggtgt acagtttgcg ctggccgtgg
    19741 tttaccccag ggcgggaaat atcggcggcg gcgggtttat ggtttacagg gcggcctccg
    19801 gcgagaccaa tacgctggat taccgcgaaa aagcgcctgc cctggcttca agagacatgt
    19861 acctggattc attgggaaat ccgggcaccg gcagtgtcct gggtcatctg gcatcggggg
    19921 tcccgggtac ggtcgacggc ctgatcacgg cccatgccaa atacggcaaa ctgtcttttg
    19981 aaaagatcat tgcaccggct attcgattgg ccgaggatgg gttccaggtg accgccgacg
    20041 aggctgccag gctgcaggac taccgggagg tgtttgtccg caccaacgga atggaaaatg
    20101 ccttcgtcaa agcaaacaaa tggaaagaag gcgactggct gatccagaag gaactggctt
    20161 ggacgctgaa gcagatccgg gaccgggggc gggcgggatt ttatgaagga ccggtcgcgg
    20221 accgcatcgt tgccgaaatg atacagggta agggaatcat tagtcacgat gacctgaaag
    20281 cctatcgttc ggtttggcgg aagccggtga tcgttccata tagagattac aaggtcatat
    20341 ccatggcgcc gccttccagc ggtggcatcg ccctgggtca gttgctgaaa atggtggagc
    20401 cttatcctct caaagactgg ggattcaggg actcccggac cattcacctc atggtagagg
    20461 cggagcgcag ggtttatgcc gaccgggccc aatacctggg ggacgccgat ttttaccccg
    20521 taccgcagga cagtctcctc aatcccaaat acctgaaata ccggatgcag gattaccgtg
    20581 aggagcaagc cagtaaaagc agcagcgtct atgcgggcaa ctttaaattg gccctggaac
    20641 attttgagac cacccacctc tccgtggtcg atcaatacgg gaatgctgtg gcggtcacca
    20701 ccactcttaa tggaaatttc ggcagcaagg tcatcgtgga cggggccggt tttttaatga
    20761 acaacgaaat ggatgatttc agcgtaaaac ccggggtgcc taatatgttt ggcctgatcg
    20821 gcggcgaagc caacgcgatc cagccgggca aaagaatgct cagcagcatg actcccacca
    20881 tcgtggaaaa aaacgggaaa ttgtccatgg tcgtcggtac gccgggaggc agtacgatca
    20941 tcacttccgt cttccaggtc atcctcaatg tattggaatg gaacatgccg atgggcgaag
    21001 ccgtggcggc cagccgcttc catcaccagt ggctgcccga tgaaatcatc ctggaagaaa
    21061 atgctttcga tccggtgctg aaatcaaaac tggaatccat gggccataaa atacgttcca
    21121 tcagttctat tggcctggtc gatgcgatcc gggtcaggcc ggacggcggc ctggaaggag
    21181 gggcagatcc caggggggat gatcatgcgg aggggtggta ggtaggaatc agtagtcagt
    21241 agtcagtagt cagtagtcag taatcagtag tcagtagttt tgctccctta acctttgacc
    21301 cttaaccgtt cacccctcaa ttgtttggga ggagcttagc tccgaccttc cgacataaat
    21361 caataatcaa taatcaataa tcgatagtca atagtcaatc tcgcctctcg ctgcgcatcc
    21421 gtaattgtat ttttgctccc ttaacccttg acccttaacc gttcacccct tccgacaata
    21481 gtcaataatc aatagtcgat agtaaatagt caatctcgct tctcgcttct ggctgctcgc
    21541 ctctcgctgc tcgtttggga ggagcttagc tccgaccttc ctctttaaga taatcgtttg
    21601 tttcctgctg caaatggata tatccggcta cattcgacaa caaacggtta atacccgaca
    21661 acaaacgatt aatacttcct ttatttaaaa aaaatcagct aatgcattga atattaaggt
    21721 tttaggtttt aatttagcaa gaaggaaaat agaacacccg gtgattcatg cggcctatgg
    21781 acacacttgt aaaaaaaata acccactatg aagatcttta tatttcctgt taattcccag
    21841 ggacaaaaac tgatcggttt ccggaccgaa cattttgatc cggtaagtat tgctatgatt
    21901 aaaaaaatac cccgtagtca ttgaaaccag ggtaccacaa gctggtgcat accttatgaa
    21961 aaagagacct ggaggcggtg ccaggaaacc ctggccgcct atgaattaat caaaatggtt
    22021 agcggagtgg tagcggataa aggagtggac cgccttttgc ctccggatcg gcaggtggag
    22081 ttcgactcct attataccca atatacatac agtggtacag cctcaatggg tactcgtcac
    22141 ctaaaaacac agaaatctat acctgcctgg cggcagacag gcccacctta ccgacactaa
    22201 taaatttcgt atgcaaagcc cgttggatgg cttggatatt gtctaaaatt tggaagatat
    22261 aagaaaatat acgcaataaa cattggtaaa taaagatgtt atgtgccata ctactcagat
    22321 gcttcattaa aagtgagcca attctaaaaa cggtctcgga aatttaagct tatggttaat
    22381 aaagaatatc tgcttaacta attatcacac ttcataatct cttaaacatg actgacgaag
    22441 aaaaatccaa aataatgatg gaagaagagc ataaactcat ggccggatat atggacactg
    22501 caaaaacatt tacccaatta agtattggtg ctcttgtcct ttctattact ttcttggaaa
    22561 aaatactggg attcaaagat aaaattccaa ttcactattt gcttttgatt gcatggttat
    22621 tctggttatt ggcggcatta tctggcgcat tttatcaata ccgtgctgta aagtggctgg
    22681 agaaactagg tattgaatcc aatttaataa aaaaacaaaa ccatgtcggc gacctcttaa
    22741 aggtatatcc atatcaagta tatggagctt ttcttgtatt tttctttctt ggaactattt
    22801 tttttgcaat atttggtgca acaataattt tgtgacaata tcccatcaat atcaacggct
    22861 taaaaagtac ggcacataac atggggtttg gcgatatggc ggctggagaa catattctca
    22921 tcattccgtt cgctgctcag ctttggtctc gtgtaaccgg ttctatcatg caatcaataa
    22981 cattcaacta acttatcttt atcaaataac aatccgggct ttggtaacac ggaataccgc
    23041 cacatcgcca agccctaacc gttagcactc attttaaaaa ctgacgatac agacaaatat
    23101 catgacaagt gacgacaaga aattatggcg agaaaggtgg cttggttgca ttaacgaatt
    23161 gacttcgctt gatcttcaaa agaaatcttg gctcgacaaa acaaatataa atccgcattg
    23221 gtcatttgta gaatttatgt gtagttattt tgacgactta ggaattgacg acaattataa
    23281 atatgcgatt gacaaaggtt ggttgactga ccaggagttt gaaataatta aagactggca
    23341 cgaagcattg gataaatacg actcaccgaa aaatgacgac tatgaccatg cagcaatttt
    23401 aaacgaccct acatggcttg acattttgca aatcggacta acaatgaaaa gcaaattaac
    23461 tccgacatta aatgagacag aaaggaagat attgactgaa gaaattaact atttgaattt
    23521 cgtttagggg tgagaaaaag gagtgctaac aagggttttg cgtcaggcgg gctgaccccg
    23581 tttcgtgcct cacggggcag gcagtgcaat cccgaaagca ttcgggatgg agctttgtgc
    23641 ttctattcaa gttcagtgcg ggttgacagt tttgtgctcc gatcccgaat gtcttcggga
    23701 ccgctcgaac ctgtctgacg tatccacccg gccaagtaca tatactcgga ttagcggtat
    23761 gaataaagaa gg
//
LOCUS       DAOQXT010000074        23698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2493_length_23698_cov_6.528317, whole genome shotgun sequence.
ACCESSION   DAOQXT010000074 DAOQXT010000000
VERSION     DAOQXT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23698
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2493_length_23698_cov_6.528317"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKM27_13000"
     CDS             complement(<1..636)
                     /locus_tag="PKM27_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08229.1"
                     /translation="MFSNYVKLAFRNLFKRKGYSLLNIFGLSIGITCCLLIFRYVSFE
                     RSFDRFVPESEQIYRLRLDAYQQGKLAWKSATVYPAFGPTLKKDFPEVEAFCRLHDAN
                     FLLSNDDKQIKFREEKGYFADPAFLDLFGIRLRSGNPATALEGPDKVMLTPGMAEKYF
                     GKEDPIGKKLVLRDADYTRVLEITGVFEELPAQSHLQIQHLMSYKTLDAINR"
     gene            complement(858..3287)
                     /locus_tag="PKM27_13005"
     CDS             complement(858..3287)
                     /locus_tag="PKM27_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08230.1"
                     /translation="MLTNYLKIAWRNLMRYRFISFINLFGLTTGLTGCLLIFNYIRHE
                     LSFDRYHEHADRIYRVTRIFRNPETGAQSLHLGTISPPFGPLLKAEFPEIEKITRLID
                     FSPLPMRYEEKMFNEQSVFLADEHLFDVFNVEVLRGNPSTALADPFTLMLSEGIAKKY
                     FGDDDPMDKSVRLNNQFNLKVTGVYKSFPSNTHMHPDILISFNTLNDSSIYGAQNLRT
                     NWGNNSFFTYLLLPEGYDYKKMEARFPDFLDKHMPRGAQARFLPSQGTALTLQKLTDI
                     HLHSHLDYEAEENGDIKRVYVFSAIALFILLIACINYMNLSTARSALRAREIGIRKTV
                     GAERKVLITQFLSESLLISWMAMLLSLGFTWLLLPWLNKISGQQLSMSTLLDPKVLPW
                     LVLVPFAVGLISGIYPALFMSSFRPVQVLKGLFTAGGSTITFRKALVTAQFAISIILI
                     ISTAVVFKQLRYMQRKSLGFEKDQIVTLPYNRQLNPAYESFRNQLISNSNIKDLTRSS
                     RIPTGRLLDAMGTRMRRGDSLAPVNVDLKYVAVDERFLSTYGVKVVAGRDFSREFSTD
                     TSAFLINEAAVQALGFQANEDALGQDIQYGNRKGKLLGVIQDFHFESMHQKIVPLILM
                     VPRQNGGFGRISIKMAGNNIPAVLAHIEKTWNGFLPEIPYEYTFLDENFDRLYQAENR
                     QGTIFTIFACLAIFIACLGLLGLSAFAISQRIKEIGIRKVLGAGTASIVGLLSKDFLK
                     LVAIAFVVAFPLAWYAMSRWLGDFAYRIRVPLWVFFLAGGIAAAVALLTIGVQAVKAA
                     RSNPVKSLRTE"
     gene            complement(3423..5855)
                     /locus_tag="PKM27_13010"
     CDS             complement(3423..5855)
                     /locus_tag="PKM27_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08231.1"
                     /translation="MIKNFFTTSWRFLWKNKSFSLINIAGLTAGILACLYILLYVYDQ
                     KNYDRHHRNGTDLFRVTSFLKLPGDQTNHLATCSAPIAPAMKNDFAEVMQFARTVGTI
                     GVSHHLLRYKENSFFETDAIYVDSTFFELFNYRFLSGDPATALRDPYSIVLNSRTALK
                     LFGTDQPVGAFIEIDNSYGKQNFKVTGVVDESLGKTHIKANLFLAMNSGGMGEYARNN
                     NSWAGNNFTSTYIRLHPEATPDALEKKLPAFLIKYGAQQLKDMGMEKVLHLQPVADIH
                     TSPGYQAEMSEPVNPFMLNVLMLIAILIQGIACVNFMNLSTARSSKRAKEVGVRKVMG
                     AGRTGLIKQFIGESALLSGISVAAAVLLLYLAMPMLNKITQTEIQFFSGNQKYIWLLL
                     AGITLVTALLAGSYPAFYLSAFNAVRVIKGNFTSHISSAGIRKSLVVFQFVLSIILIT
                     GILVIYQQMRFIQNKDLGFEKEQRIVLNFRTEEARRQMDAFRNELIGLTGIKGVCKSN
                     NYPSQFVYNDIPVFLQGGGVQDSKDPEFIMVDEHFTEAMGIKVLAGRPFHDHDSGKVL
                     INETLARELGLDPQHAEGTRLFSQVTLADQRPLSFEIAGVIADFNFNSLHRAILPFML
                     LYGSDPENLTHLIVATSSDDYGQLLSHMESAWLKFLPAVPFDYVFLDDQVQKQYQADL
                     TFSKIIGTFALFAILISCLGLFGLAAFRAEQSTKEIGIRKVLGASVASVFHLLAKDFL
                     ILVSISFLIAAPIAWWAMQSWLKAFEYRISVSGWVLAAAGLATLIIALCTISYQALKA
                     AVANPVKSLRTE"
     gene            complement(5961..8378)
                     /locus_tag="PKM27_13015"
     CDS             complement(5961..8378)
                     /locus_tag="PKM27_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08232.1"
                     /translation="MYKNYLFSAWRNLIKNKAFSFINISGLALGLSVCLLLMLFIRSE
                     FSYDRFHAKSERIYRAWLHEVYEGQTFTNTQTPIPLGPVLKENLPDIEAFCRVYQFNS
                     LLEQQGNRFNESVNMVDTSFFRVFDFHLMEGDPGQAFPNSNSMVISERMEEKYFGSGK
                     GMGKTLEMQLGQTKVLFTVSAISRESPLESSIRFDFLIPHSNEHHLFSERARTQGWTN
                     VFEETYLLLGEGKSGDAVEAQIPALVKRISGDQYVEGEYNVYLQPITDIHLNNKLPAG
                     NAPVSDPMYSYVLGFLGILILLIACINFITLSLGRSSTRALEVGVRKVLGAARTQLIR
                     QFWGEALMFTFIALGIAIGLSYLLLGPFNQLANRQLTLHLDGFTVMFLLALVVLVGLV
                     SGIYPAMVLSSFLPVKVLKNRMPEGFRIGLFRKSLVTGQFFSAIVLMAGTLVMWQQFR
                     FIRHTSLGYEKEYLVVVPTNKSRAEGMALAERFKIKLKSNPQVVGSTVNLFSFAQPGW
                     MNIGYEDDQGIYRNFRMNAIDADFIGTMGVELAAGRGFSSDNPADLAGSMLVNERLVE
                     EYGWDDPIGKKLPGRFNQQIIGVVKDFHFESLHSPIKPLVLVLQPDSILRAANDVGFT
                     FSPQPRINVRLKAGSVREQIEGLKQNWQEVAGGQEFEYSFLDEALTDMYKNEQRVSSM
                     VSWASLLSIFIASLGLFGLITLAVNRRIKEIGIRKVLGADVKGIVFLLSKDFTGLLLF
                     AMLFAAPVGWWALDQWLQSFAYRIRLQPWVFLLAGVSVFAIAWITMSIQAMRAAKTNP
                     VKSLRTE"
     gene            complement(8575..10938)
                     /locus_tag="PKM27_13020"
     CDS             complement(8575..10938)
                     /locus_tag="PKM27_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08233.1"
                     /translation="MMMHEFKIAFRSVMKNRWFSLINVAGLALGLAVFMTILLWVKYE
                     WSYNDFHRDGDRIASVMTNLKLEGGEISTFPAVPPLLAKALSKDLPGIEYACTSSWGD
                     QRQVSYGDLHFMEYGLYVSPEFLKIFSFPLIKGDQDKVLTEPNTILITEKLAEKYFKD
                     QDPVGQTLVIDQATTYTVTGVLKDVPRNATLSFDFLMPVQDYIRTVMGGNETWETNNM
                     RAYVKLHEGADREAVARSIRKFQDGYTDKQPNSELALFYLEDWYLRMDFKNGKYAGGG
                     RIADVRLFTLIALVILLLACINFMNLSTAKSTRRAREVGVRKTIGAGRGSLVRQFYGE
                     SLLFSFIAGLCALVLVSLALPFFNSLLRKQIAIDFTDPSNYLVFAGIIGLTGLVAGSY
                     PALALSSFSPVQVLKNHISSSGLGFSQLRKGLVLIQFMVSMILLAGTFSVVKQLRFLR
                     EKPLGFDKDHLIWFPNQIPVEKHPAALNLFSAIPGVSHAALASMTFTQSNNRGSEVNW
                     PGKLPGQDVFFSFIAGSQEIVETMGLTLAEGRAFMPGQYADTGAFILNEEAVRRMGLE
                     HPVGQILETHGESGRIVGVVKDFHFESLHSPIAPVIIMCRPGWTWLYYVRTTGRDVQR
                     VLHDLEAAYRQLAPGFVFDYNFQDKEYDRLYRSETQMSSLVQWSSFLALFISGLGLLG
                     LTLFTLERKTKEIGIRKVLGATWRDILTMVTNEFMLLTGLAAVFALPLAYLMVSQWLN
                     RYAFRADLEWWIYLMPVAITALITLVTVGSQAIRASAANPVKSLRTE"
     gene            complement(10950..13355)
                     /locus_tag="PKM27_13025"
     CDS             complement(10950..13355)
                     /locus_tag="PKM27_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08234.1"
                     /translation="MLIRNYLKVTWRSLFKNRLFTFLNILGLSSGLGIALLLLIYVKD
                     EKSFDRYHTQSDRIYRVGLTATFDGKNMDWANTPNAVGPAMKAEIPEVAEQARLLYHD
                     YGKTAFITSGQKKFVEKKLYWSDPGLFRIFDLPFLKGDPSTALDRPNTVVISSSLADK
                     YFGAEDPLGQVLKVDNKYDLEITGVYSDFPGNSTLDPALIGSFSSIEWASKNLVWSNA
                     SFETYVLLETGALEEKVEQSMLRLLESKVEADNRWFTIWLQPLKNIHLGSAGITNAST
                     TRTGDPGQVKILGILALVVLLIASMNYMNLATAKSQLRFREVGINKTIGAGWFQLAGR
                     FYLETALVTGVSVLVACGMVAAGLPLLNRIAQKNLSAGSLLSPDLLLITAAITLCLVL
                     VAGSYPAFYLSSFRAKSLLQTSFRKNTGAGLFRRTLVVVQFSASLILIVGALLFYKQL
                     EFIRQKNLGYKPEQVVAITTAAAENTEQIQALRDYYQNLSSVVSVCRAQIWPGNGGSG
                     RTLRNILKDTREQDGLMIKTNRVTSEFADVLGLKLLAGHPIRSERIPGDTIVQVVLNR
                     TAANYLGYTPEEAIGKKAYSLFGHDRAEIAGVVEDFHFESFHQPIGAYAFHNNDSEWR
                     PYLLVKLNTQNLPATMQQLEKAFNTHIPNSAFDYTFIDQFLNTLYASEQRTAKVVLLF
                     ASLTILIACLGLFGLAAFTAEQRIKEIGVRKVLGASVTDITALLSWDFLKLVGLAILI
                     ASPLAWYGAHLWLQDFAYRVEIGWGLFVLAAVLSLSIAFFTVGFQSLKAALSNPVRSL
                     RTE"
     gene            complement(13506..15950)
                     /locus_tag="PKM27_13030"
     CDS             complement(13506..15950)
                     /locus_tag="PKM27_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08235.1"
                     /translation="MYKNYLLTAWRNLIKNKTFSILNVTGLAIGIACFILIANYIVDE
                     VSYDRWNKYADRLYRVDADIRIGGSELNLAVSSDPMGPTLKQDYPQVEEFVRLYASSG
                     SKLIKKGSEYIEEGGVVHADSTLLRIFPFEVLAGDAQRPLHGPGKVILSETAARKYFG
                     NTGLEEVIGKTLETDDRDKFYTVTAVIRDVPRNTHFDKDLFFSIDNVDYNWGSYLSNN
                     FTTYIRLQEGADPLEFNKNFPGFLEKYVIPQVQQFAPIKSMDEFKAAGNKLEYFLFPV
                     TKIHLYSNRVAELAANSNIQYVYIFSAVALFILLIACINFMNLSTARSANRAREVGVR
                     KVLGTGRNNLISQFLTESTLIAFFATAAGIGMALLLLPFFNDLSAKTFGMKELFKPGW
                     LAVYLSLPFLLGLIAGIYPAFYLSAFRPIAVLKGKMEGSLNKSGLRSALVVFQFATSI
                     LLIIGAIVVYNQLKYIQSKNIGFNKDQVLVIDGTGALRPNTEAFKNEISKLSGVTAST
                     FAGYLPVSNSSRSDNTFFKDAVPDVNRGLNMQVWRIDEDYIPLMGMEILAGRNFSKER
                     GTDSSAIILNEEAVRLLGFESNPLDQKIYGGSRIEQNQVEAFHVIGVVKDFNFESLRQ
                     KVGPLCFFLGKADGTTAFKVNTGNIQQLIGQIEDTWRTMAKGAPFSYRFLDQSFDEMY
                     RAEQRVGKIALSFSILAIFIACLGLFGLATFIAEQRTKEIGIRKVLGAEVPDIITMLS
                     RDFIRLVLIAAVVAFPLGWFLMNRWLQDFAFRINISGWVFVIAGLAALVIALFTISFQ
                     ALKAALMNPVKSLRTE"
     gene            complement(16025..16384)
                     /locus_tag="PKM27_13035"
     CDS             complement(16025..16384)
                     /locus_tag="PKM27_13035"
                     /inference="COORDINATES: protein motif:HMM:NF008912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR08236.1"
                     /translation="MKIEKFEDLAVWNEALELAKEIYLELKASRDYSFRDQLQRSAVS
                     ISSNIAEGFERQTNKEFIQFLYIAKGSCGELRTQVHLAMRIGILNQEKSVAMLEKTKK
                     ISSMLYGLIKVRKEKFK"
     gene            complement(16473..17150)
                     /locus_tag="PKM27_13040"
     CDS             complement(16473..17150)
                     /locus_tag="PKM27_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR08237.1"
                     /translation="MIKITDLEKTYRTEEVETIALNKVSFDVKTGEFIAIMGPSGCGK
                     STLLNILGLLDDMDSGSYLFDGIEVAKFNERKRADLRKHNIGFVFQSFNLIDELSVFE
                     NVELPLIYTGVKSDERKRRVEEVLDKMQIMHRRNHFPQQLSGGQQQRVAVARAVVNKP
                     KLILADEPTGNLDSSNGNEVMQLLTDLNEQGTTIIMVTHSEHDARYSHRIVRMLDGRT
                     VMENILV"
     gene            17313..17666
                     /locus_tag="PKM27_13045"
     CDS             17313..17666
                     /locus_tag="PKM27_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR08238.1"
                     /translation="MRNFYDLKVWKLSHELTLKIYGTVISFPPEELFGLRSQMRRSSS
                     SIPTNLAEGCGRNSNPDFKRFVNIAIGSTSELEYQLLLAKDLKYLNEPTFIELTNNVI
                     EIRRMLISLYKKAPL"
     gene            complement(17741..18994)
                     /locus_tag="PKM27_13050"
     CDS             complement(17741..18994)
                     /locus_tag="PKM27_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNR08239.1"
                     /translation="MDRPIAKKTWTTQRILSILGLLALGVIIYMAVRSSSGKSKLNVD
                     TERLMISEVVEGPFKEFIPINGVVMPITTIYLDALEGGRVEEKLVEDGAMLKRGQPIL
                     RLSNTDLELTLANQETAVFEVLTQMQNTKNNAEQNTIRQLNQMAEVTNALEEASRVYH
                     LNKKLYEQKAIGFQDFKSSENLYNYQVRRKNLTEQILTQDSLSTRTQLAQMEESYARM
                     QNALQLMRRKVGDLIVRAPVDGQLTSLDAEIGQSKQKGERLGQLDVLSGYKIRADIDE
                     HYISRVFTGLEGDFTFAGKKYFLKIKKIYTQVTGGRFQVDMEFTGEVPQGIRRGQTLQ
                     VGLALGDESVVILVPKGGFFQTTGGNWIFKVNEAGNLAFKQDITLGRQNPNHYEVLSG
                     LKPGDKVVTNSYETYGKMEELVLQR"
     gene            19233..20567
                     /locus_tag="PKM27_13055"
     CDS             19233..20567
                     /locus_tag="PKM27_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNR08240.1"
                     /translation="MNLRESSILIVDDDPDVLTAVRLLLKPLVRSISFSRYPDEIKSL
                     LKEQDFDIILLDMNFRSGIHSGNEGLFWLKEIKKIKPQVMVILITAYGDIDLAVRSLK
                     EGAEDFILKPWQNEKLINSLHAAFKKQVKSAPAPPDAIRLLGESEKMKDLMVKVSKVA
                     PTDANVLILGENGTGKDVLAHQIHYHSSRADKPFIKVDLGALTESLFESELFGHKKGS
                     FTDAREDRVGRIEAAQGGTLFLDEIGNINLTQQAKLLTVIQNRHITRIGSNIPSQVDI
                     RLICATNLPMNELANEQRFRRDLLYRINTVDLHLPPLRERDKDIEILAKYFLQTYSAK
                     YLKPDLKLGRGALEALLSYPFPGNIRELQYTIERAVILCDKDELSASEIIFSGIESSQ
                     KLADAGDFKLSSVEKNTVIKVLEKHSGNITKAAKELGITRTALYRRLNKYEI"
     gene            20659..21996
                     /locus_tag="PKM27_13060"
     CDS             20659..21996
                     /locus_tag="PKM27_13060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR08241.1"
                     /translation="MAFERFEWKIIRRVVILLVTALAFTWLLVNSYYIYMLIPGVVLG
                     YLAYEMYVFNRKAYDEIDQYVESVHYRDFSRHFNVKEAPKELKSLREGFNEINDTIKD
                     ISLEKETHYQYLQKVLEIINTGIISYEEQGGQVIWLNETLKKMLQVPYLRTIHSLEKR
                     NPLLYRKIIDINSGNQDIVKLSEGLKELKVLLNSTAFLLEGKKYKLVAIQNINEAIDE
                     TEANAWQKLLSVMTHEIMNSVAPIASLADTLKTRLQDATPDEMEDLGVGIETIKKRSE
                     GLLRFAQTYRNLNKVSQPQIKAIPLGELFSTMYNLMNPTLEQKNIDLDIILKDPNLVL
                     HADPNLIEQVIINLLLNAKDALLDKPDARIMLSAEESNNRLVLKVADNGRGIPAEVAD
                     KIFVPFFSTKTNGSGIGLSLSKQIMLMHKGNIHYASRENEGTVFELVFPKLPA"
     gene            complement(22005..23630)
                     /locus_tag="PKM27_13065"
     CDS             complement(22005..23630)
                     /locus_tag="PKM27_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08242.1"
                     /translation="MKNKIRHLIFLMSWLFAGREGYCQGFGIHLDRSVYISGDALRFK
                     IYYLDKLDKNPGLLYVRLLNAKQFTLDRQLLKLDSLGAEGCIYLPSKLETGRYFLHFY
                     LVWDSRFGTVPKFESGFLPVDVYNDFEEEITDLGRPGPTFVSDGREIYARVKRRSRVQ
                     LDLNTVSNPAGGIFQNGSVSVFQYLPGSPDPTRLMQFNRSPDINPGIDELRTEPVLEG
                     QLIDPVDQSLVSEKYLSLFVPHLGSFQRFSADSGRFKLKLPAFEGVQAVQALSLNPNQ
                     SRPLEIKPDDFNPALPLNTIEGFPVPAPSVQAYLQLNYKRRIVHDFYKIPGEQAGPPV
                     ESFKSLVPDRVYQLKDFQQIHTVGDFIREVMLDARSSAVMDDRNKLRLRNRIKKELFR
                     WPAWIMVDGLFAGKESAVFEQEVSQVNSIGIFNKESTILSQLDTLMKHSGLLAIQGAQ
                     LKDHAARYGCLALPVQGWSTGSPWIYRPPSDHREPDLRSLLYWDANVKANEDGQVRIS
                     FHTGDLTGLFEIKLVGYDRLHRLVSKTFLVEVE"
BASE COUNT         5968 a         6079 c         5940 g         5711 t
ORIGIN      
        1 gcgattgatc gcgtccaggg ttttgtaaga catgaggtgt tggatttgca ggtgggattg
       61 ggcgggaagc tcttcaaaga cccccgtaat ttcaaggacc cgggtataat ccgcgtcccg
      121 caataccagt tttttcccga taggatcttc cttgccaaaa tacttttcgg ccatacccgg
      181 agtaagcatc actttatccg ggccctccag ggcggtggcc gggttgccgc tccgcaaccg
      241 gatcccaaaa aggtccagga aggccgggtc tgcgaaataa cccttctcct ccctgaattt
      301 gatttgttta tcatcgttgg agaggaggaa gttggcatca tgcaaacggc agaaagcctc
      361 cacttccggg aaatcctttt tcaaggtcgg gccgaacgcc gggtagacgg tagccgattt
      421 ccacgcgagc ttcccttgct gataggcatc cagccggaga cggtagatct gttccgattc
      481 cggtacaaaa cggtcaaaac tccgctcgaa ggaaacatac ctgaaaatca atagacaaca
      541 ggtgatgcct atcgaaaggc cgaaaatatt caagagggaa taccctttcc gcttaaacaa
      601 gttcctgaag gccagtttca catagttgct gaacatatca ttaggtattt tatgattttt
      661 aaacccggat ttcttaaatc ccggcacatc tcataccaaa ggcaacaatc attgaaaatc
      721 aagggaataa aaaagatcaa agtaaaaaac cgtccgtttt cgaacagtag gtgactggtt
      781 tccgttaatt ccttaattcc ttaattcctt aattccttaa ttccttaatt ccttaattcc
      841 ttaattttta attccactta ctccgtcctc aaactcttta ccggattgga ccgggccgcc
      901 ttgacagcct ggactccaat ggtcagcaaa gcaaccgcag ccgctatgcc gccggctaaa
      961 aagaataccc aaagcggtac gcggatccgg taggcaaaat ctcccagcca ccgcgacatg
     1021 gcataccagg ccaggggaaa ggcaaccaca aaggcaatgg ctaccagttt gagaaaatct
     1081 ttggacaaca ggccgacgat actcgccgtg cctgcaccga gtactttccg gatcccgatc
     1141 tctttgatcc gctggctgat ggcgaaagcg gaaagcccga gcaagcccag gcaggcgata
     1201 aagatggcca ggcaggcaaa aatggtaaag atggtgcctt gcctgttttc cgcctggtaa
     1261 agccggtcga aattttcatc cagaaaagta tactcgtacg ggatctccgg gagaaagcca
     1321 ttccaggttt tttcaatatg agccagcacg gcaggaatgt tgttgcctgc cattttgatg
     1381 gagatccggc cgaaacctcc attctgtcgg ggaaccatca ggatgagggg cacgattttt
     1441 tggtgcatgg attcaaaatg aaaatcctgg atcaccccca ggagtttccc tttgcggttg
     1501 ccgtattgga tatcctggcc cagggcatct tcattggctt ggaatcccag ggcctggacc
     1561 gcggcttcat tgatcaggaa agcgctggta tcggtgctga actccctcga aaaatcacgg
     1621 ccagccacca ctttcactcc ataggtggaa agaaaccgtt cgtccaccgc cacatatttc
     1681 aggtccacat tcaccggggc caggctgtcg ccccgcctca tccgggtgcc catggcatca
     1741 aggagccggc ccgtagggat acgcgaggaa cgggtaaggt ctttgatatt gctgtttgaa
     1801 atgagctggt ttcggaagga ctcgtaagcc gggttcaact gcctgttgta aggcagggtt
     1861 acgatctggt ctttttcaaa gcccagggat tttcgctgca tgtacctgag ctgtttaaat
     1921 acgaccgccg tactgatgat gagtatgatg gatatggcaa attgtgcggt gacgagggct
     1981 tttcggaaag tgatggtcga cccgcccgcg gtaaacaatc ccttgagcac ctgtaccggt
     2041 ctgaaagaag acataaagag ggccgggtaa atccccgaaa tcaggccgac cgcaaaaggg
     2101 accaggacca gccagggcag gacctttgga tccaggaggg tactcatgga caattgctga
     2161 ccggaaattt tattgagcca gggcaataac agccaggtaa agcccaggga taaaagcatg
     2221 gccatccagg agatcagcag ggactcgctc aggaactgcg tgatgagcac cttgcgttcg
     2281 gcccccaccg ttttccggat gccaatctcc cgggcccgga gggctgacct ggcggtcgag
     2341 aggttcatat aattgatgca ggcgatcagc aggataaaca aggcgatggc tgaaaataca
     2401 tatacccgct tgatgtcgcc gttttcttcg gcttcataat ccaggtggga atgcaggtgg
     2461 atgtcggtca gtttttgaag ggtgagcgcc gtgccctggg aaggcaaaaa ccgggcctgg
     2521 gcgcccctgg gcatatgttt atccaggaag tccggaaacc gggcctccat ctttttataa
     2581 tcgtaaccct cgggcaggag caggtaggta aaaaacgagt tgttgcccca attggtgcgc
     2641 aggttctggg ccccatagat ggacgaatcg ttgagggtat tgaaagaaat cagaatgtcc
     2701 gggtgcatat gtgtgttgga aggaaaggat ttatacaccc cggtgacttt cagattaaac
     2761 tggttgttca gccgcaccga cttgtccatc ggatcatcgt ccccaaagta ttttttcgcg
     2821 atcccttccg aaagcatgag ggtaaacggg tcagccaggg cggtggaagg atttcccctc
     2881 aagacctcca cgttgaatac gtcaaacaaa tgttcgtccg ctaaaaaaac actttgttcg
     2941 ttgaacatct tttcttcata acgcataggc aatggactga aatcgatcag gcgggtgatc
     3001 ttttcaattt ccgggaattc agcttttaag aggggcccaa agggcggaga gatcgtcccc
     3061 aggtgaaggc tttgggctcc ggtttccgga ttcctgaaaa tccgggtcac ccggtagatg
     3121 cggtccgcat gctcatggta ccggtcaaaa ctcaactcgt gccggatgta attgaagatc
     3181 agcaggcaac cggtgagtcc ggtggtaagg ccaaacaaat tgatgaagga gataaagcgg
     3241 tacctcatca ggttgcgcca ggcgatttta aggtagttgg tgagcatgga gttttacagt
     3301 taatcagttt gacagttaat caatttatca atttatcagt ttgacagtta atcaatttat
     3361 caatttatca gcccgtcggg agcagatatc tcattgttcg catctcgcat ctcgcatctc
     3421 cactattccg tcctcaggct tttcaccgga ttcgcaacag ccgctttcag cgcctggtag
     3481 ctgatggtac aaagggcgat gatcaacgta gccagtccgg cagcagccag tacccagccg
     3541 cttacggaaa tcctgtattc gaaagctttg agccagcttt gcatggccca ccatgcgatc
     3601 ggggccgcga tcagaaacga aatgcttacc aggatcagaa aatctttggc caacagatgg
     3661 aaaacggaag caacgctggc gcccaatact ttgcggatcc cgatttcttt ggtgctctgt
     3721 tcggcccgga atgcggccag gccgaagagg ccaagacatg aaatgagaat ggcaaacaag
     3781 gcaaaagtcc cgatgatctt cgaaaaagtg agatcagcct ggtattgttt ctgcacctgg
     3841 tcgtccaaaa acacgtaatc aaagggcacc gcgggcagaa atttcaacca ggccgactcc
     3901 atatgggaaa gcagctgccc gtagtcgtcc gatgaggtcg ccacgatgag gtgggtcagg
     3961 ttttccggat cgctcccata tagcagcatg aaaggcaaga tggctctgtg caacgaatta
     4021 aaattaaaat cggcgattac gccggcaatt tcaaaggaca atggtctttg gtctgcaagg
     4081 gtaacttgtg aaaacaggcg ggtgccttct gcatgctggg ggtccagtcc caattccctg
     4141 gccagcgtct cgttgatcag caccttgccg gaatcatgat catggaaagg ccggcctgcc
     4201 aacaccttga tgcccatggc ttccgtaaaa tgttcgtcca ccatgataaa ctccggatcc
     4261 ttcgaatcct gcaccccacc accctgcaga aagacgggga tgtcattgta tacaaactgg
     4321 ctgggataat tattggattt gcagaccccc tttataccgg tgaggccgat cagttcattg
     4381 cggaaggcat ccatttgcct ccgggcctct tccgtccgga aattcaggac aatacgctgt
     4441 tccttttcaa aaccaaggtc tttgttctgg ataaaacgca tctgctggta tatgaccagg
     4501 atgcctgtaa tcaggatgat cgacaggaca aactggaata ctacgagcga tttgcggatg
     4561 ccggcggacg aaatatggct ggtaaaattc cctttgatca cccttaccgc attaaaagcg
     4621 gacaaataaa aagcgggata gctgccggcc agcagggccg tgaccagggt gatgccggcc
     4681 agtaacaacc agatatactt ttgatttccc gagaaaaact gaatctccgt ctgggtgatt
     4741 ttgttcagca tgggcatggc caggtataat aagagaaccg ctgcggctac cgatatcccg
     4801 gacaacaaag cggattcccc gataaattgt ttgatcagcc ctgtcctgcc ggcccccatg
     4861 acctttcgca ctccgacttc cttggctcgt tttgaagacc ttgccgtgga aagattcata
     4921 aaattcacgc aggcaatacc ctggatgagt attgcgatca gcataagcac gttaagcatg
     4981 aatgggttta ccggttcgga catttcagcc tggtagccgg gggaagtgtg aatatcagcg
     5041 accggctgga gatgaagtac cttttccata cccatgtctt tcaattgctg cgcgccgtat
     5101 ttgattaaaa aggcaggaag tttcttttcc aatgcgtccg gagtcgcttc cggatgaagc
     5161 cggatatagg tcgaagtaaa attgttgccg gcccaggaat tgttgttcct ggcatactct
     5221 cccatgcctc cgctgttcat ggccaggaaa agatttgcct tgatatgggt tttacccagg
     5281 ctttcgtcca ccaccccggt aaccttaaaa ttctgtttgc cgtaggaatt gtcgatctcg
     5341 atgaatgctc cgaccggctg gtcagttccg aataatttca gggcagtacg ggaattcagc
     5401 acaatggaat agggatcccg caaggccgta gccggatcac ccgagagaaa ccggtaattg
     5461 aagagttcaa aaaaggtgga atccacatat atggcatcgg tttcaaaaaa tgaattttct
     5521 ttatacctga gcaagtggtg gctcactccg atggtgccga ccgtccgtgc gaattgcatc
     5581 acttctgcaa aatcattttt catggccggg gcgatcgggg cggaacaggt ggccagatga
     5641 ttggtctggt ctccaggcaa tttcaggaag gaagtgaccc ggaacagatc cgtcccattc
     5701 cggtgatgac ggtcatagtt tttctgatcg tatacataaa ggagtatata caggcaggcc
     5761 aggatgcctg ccgtcagccc ggcaatgttg atgaggctga acgatttatt cttccataag
     5821 aacctccagg aagtggtaaa aaagtttttg atcatggtct attatatttt tagtagtcag
     5881 tggtcaggaa tcagtagtca gtagtcagta gtcaatggtc aatagtcaat agtcaatagt
     5941 caatagtcaa tagtcaatag tcattcggtt ctgaggcttt tcaccgggtt tgttttagcg
     6001 gccctcatcg cctggatact catggtgatc caggcgatgg caaacaccga taccccggcc
     6061 aataagaaca cccaaggctg aagacggatc cggtaggcaa agctctgcaa ccattggtcc
     6121 agggcccacc aaccgaccgg ggcggcgaac agcatggcaa acaacaacaa tccggtaaag
     6181 tctttggaaa gcaggaacac aatgcccttc acgtcggcgc ccaatacctt gcgaatccca
     6241 atttctttga tccgccggtt taccgccagg gtgatcaggc caaacagtcc cagggatgcg
     6301 ataaaaatcg agagcaggga ggcccagctt accatggaac tgacccgctg ttcattcttg
     6361 tacatatcgg tgagcgcttc gtccaggaag ctgtattcga attcctggcc acccgccact
     6421 tcctgccagt tttgttttaa tccttcgatc tgctcccgga cggaccctgc tttcaggcgg
     6481 acatttatcc tgggctgggg agaaaaggta aagcccacat cgttggcggc acgcaggatc
     6541 gagtccggtt gaaggaccaa gaccaggggc tttatggggg aatggaggga ttcaaaatga
     6601 aagtctttca ccacaccgat gatttgctgg ttgaaccgtc cgggcaattt tttaccaatg
     6661 ggatcatccc aaccatattc ttccaccagc cgttcattga ccaacatgga cccggcgaga
     6721 tccgccggat tgtcgctcga aaatccacgg cccgctgcca gttcgacacc catggtcccg
     6781 ataaaatcgg catcgatggc attcatccgg aaattgcggt aaatgccctg atcgtcttca
     6841 taacctatat tcatccaccc cggttgagca aaactgaaaa ggttgacggt gctgcccact
     6901 acctggggat tgcttttgag tttgatttta aaacgttccg ccagggccat gccctctgcc
     6961 ctggatttat tggtggggac gaccaccagg tattcttttt cataacccag ggaggtgtgg
     7021 cgaataaaac ggaactgctg ccacatgacc agggtccctg ccataagtac gatggcgctg
     7081 aaaaattgcc cggtgaccag gcttttcctg aaaagaccga ttctgaagcc ctccggcatc
     7141 ctgtttttca acactttgac gggaaggaag gaagaaagca ccatcgcggg atagatccca
     7201 gaaaccagtc cgaccaaaac gaccagcgcc aacaggaaca tcaccgtaaa cccatccagg
     7261 tggagggtga gttgcctgtt ggccaactgg ttgaaggggc caagcaacag gtaagaaagg
     7321 ccaatggcta tccctaaagc aatgaaggta aacatcaggg cttcacccca aaattgacgg
     7381 atcaattgcg tacgcgccgc accaagcacc ttgcgtaccc ctacttccaa agcacgggtg
     7441 gaggatcttc ccaaagacaa ggttataaaa ttgatgcagg cgatcagaag aatgagtatc
     7501 ccaagaaaac cgagtacgta ggaatacatg ggatcactga ccggcgcatt gccggcgggc
     7561 agcttgttgt tgaggtggat gtcggtgatg ggctggagat aaacattgta ttcgccttcg
     7621 acatactggt cccctgaaat ccgttttacg agggcaggga tctgggcttc tacggcgtcg
     7681 ccggacttgc cctctccaag cagcaaatac gtttcttcaa atacattggt ccatccctgg
     7741 gtgcgcgccc tttcgctgaa cagatggtgc tcgtttgaat gcgggatgag aaaatcaaaa
     7801 cggatgctgg attccagggg ggattcccgg gaaatggccg aaacggtaaa caataccttg
     7861 gtctggccca gttgcatttc cagggttttg cccatgccct tcccggagcc gaaatatttc
     7921 tcttccattc tttcagaaat caccattgaa ttggaatttg gaaaggcctg gcccgggtcg
     7981 ccttccatca aatgaaaatc aaagacgcgg aaaaaactgg tatccaccat atttaccgac
     8041 tcgttaaacc ggttcccctg ctgttccaaa agggaattaa actgatatac gcggcaaaac
     8101 gcttcgatgt ccggaaggtt ttccttgagc accggtccca gggggatggg agtctgcgta
     8161 ttggtgaagg tctggccttc atagacttca tgcagccagg cccggtagat ccgctccgac
     8221 tttgcatgga aacggtcata actgaattcg ctccggataa acagcatcag cagaaggcag
     8281 acgctgagcc ccagggccag tcccgagatg ttgataaagg aaaaggcctt gtttttgatc
     8341 aggtttctcc aggctgaaaa aaggtaattt ttatacatgg aacggtttgt cagtttatcg
     8401 gttaatcggt ttatcagtta atcagtcagt ccggagcagg catctcgcat ctcgtatctc
     8461 gtatctcgca tctcgcatct cgcatatcgc atctcgcttc tcgcatctcg catctcgcat
     8521 ctcgcttctc gcatctcgct tctcgcatct cgcatctcgt ttctcgcttc tcaactattc
     8581 cgtccttagg cttttcaccg gatttgcagc ggaagcacgg atggcctggc tgcccaccgt
     8641 gaccagggtg atcaaagcag tgatggctac cggcatcagg tagatccacc attcgaggtc
     8701 tgccctgaat gcgtaccggt tcagccattg cgaaaccatt aaataagcca gtgggagtgc
     8761 aaagaccgcc gcaaggccgg tcaacagcat aaattcattg gtgaccatag tcaggatatc
     8821 ccgccaggtg gcgcccagca ccttacggat cccgatttcc ttagtttttc gttccagggt
     8881 aaacagggtc aggcccagca aacccaaacc ggatatgaac aaggcaagga aggaagacca
     8941 ttgcaccagg gaagacatct gtgtctcact gcgatagagc cggtcatatt ccttatcttg
     9001 aaaattatag tcaaagacga aacccggtgc cagttggcgg taggcggcct ccaaatcgtg
     9061 cagtaccctc tggacatccc tcccggtggt ccgtacataa tacaaccagg tccatcccgg
     9121 cctgcacatg atgatcaccg gcgctatagg gctatgcagc gattcaaaat gaaaatcttt
     9181 caccacgccc acgatcctgc ccgattcgcc atgggtctca aggatctggc cgaccggatg
     9241 ttcaaggccc atacgccgga ccgcttcctc attgaggatg aaagcacctg tgtcggcata
     9301 ttggccgggc ataaatgcac gtccttctgc aagggtcaga cccatggtct ccacgatttc
     9361 ctggctcccg gcaataaaac tgaagaaaac gtcctgtccg ggaagtttgc ccggccagtt
     9421 cacttcagaa ccccggttat tggactgcgt aaaggtcatg gacgcaagcg ccgcatggct
     9481 gaccccggga atggcagaga acaaattcaa agccgccgga tgtttttcca ccgggatttg
     9541 attgggaaac catatcaggt gatccttgtc gaagcccagt ggtttttccc tcaggaaacg
     9601 gagttgtttg accaccgaaa acgttccggc cagcaagatc atcgaaacca taaactggat
     9661 caggaccagg cctttcctca actgtgaaaa tccaagtcct gaggaggaaa tatggttttt
     9721 caaaacctgg acaggtgaaa aggaagaaag agcaagggca ggataactgc ctgcaaccag
     9781 gccggtcagg ccaatgatcc cggcaaatac gagataattc gagggatcgg taaaatcaat
     9841 cgcaatctgc tttcgcagca gggaattgaa gaagggcagg gccagggata ccaggaccag
     9901 ggcgcacaag ccagctataa aagaaaataa aagggactcc ccgtaaaact gcctgaccag
     9961 ggacccgcgg ccggccccaa tggtcttccg cacaccgact tccctggccc tccgcgtgga
    10021 tttggcggta gataaattca taaaattgat gcaggccagc aacaggatga ccaatgcgat
    10081 gagcgtaaaa agccggacgt ccgcgatccg gcctcctcct gcgtactttc cgttcttaaa
    10141 atccatccgc aaataccagt cctccagata aaataaggcc agttcggaat tgggttgttt
    10201 atcggtgtag ccgtcctgga atttccggat ggaccttgcg acggcctccc ggtcggcgcc
    10261 ttcatgcagc tttacatagg cccgcatatt gttggtttcc caggtttcgt tcccgcccat
    10321 gaccgtgcgg atatagtcct ggaccggcat caggaaatca aaactaaggg tggcgttgcg
    10381 ggggacatct ttcagcactc ccgtcacggt ataggtcgtg gcctggtcga tgaccagggt
    10441 ttggcctacc gggtcctggt ctttgaaata tttttcagcc agtttttcgg tgatgagaat
    10501 ggtattgggt tcagtcagca ctttgtcctg atcccccttt atcagcggaa aagaaaatat
    10561 ttttaaaaat tcagggctga catacaatcc gtattccata aaatggaggt ccccgtaact
    10621 cacctggcgc tggtcacccc aggacgaagt gcaggcatat tcaatgcccg gcaggtcctt
    10681 gctgagggct ttggcgagca ggggcggcac cgcaggaaag gtggagatct ctcctccttc
    10741 gagcttaagg ttggtcatca ccgatgcaat ccggtcgccg tcgcggtgaa agtcgttgta
    10801 actccattcg tatttcaccc aaagcaggat ggtcataaat accgcgagcc ccagggcaag
    10861 gccagcgaca ttgatcaatg aaaaccaccg gtttttcatg accgaacgga aagcgatttt
    10921 aaattcatgc atcatcatgg acagtttgtt tattcagttc gcagggatcg aacagggtta
    10981 gagagagccg ccttcagcga ctgaaaacca acggtaaaaa aggcaatgga cagggacaat
    11041 accgcggcca gcacaaacaa gccccagccg atttcgaccc ggtaggcaaa gtcctgaagc
    11101 cacagatgcg cgccatacca ggccaggggc gaagcgatca ggatggcaag gcctaccagt
    11161 ttgaggaaat cccaggaaag gagggcggtg atatcggtca cgctggcgcc taacactttc
    11221 cggaccccga tctccttgat tctttgttcg gcggtgaagg ctgccaggcc aaataatccc
    11281 agacaggcga tgaggatggt cagagaggca aagagaagga ccactttggc agtcctttgt
    11341 tcggatgcgt acagggtatt aagaaactga tcaataaagg tatagtcgaa agcagaattt
    11401 ggaatatggg tgttgaacgc cttttccaac tgctgcatcg tggccggaag gttttgggta
    11461 ttcagtttca ccagcaggta gggtctccat tcgctgtcgt tattatggaa ggcataagcg
    11521 ccgatcggct ggtggaagga ctcaaaatga aagtcctcca ccacgcccgc gatttcagca
    11581 cggtcatggc caaacaggct ataggccttt ttgccaatgg cttcctccgg agtgtaaccg
    11641 aggtagtttg ccgccgtgcg gttgaggacg acttgtacga tcgtatctcc cggtatcctt
    11701 tcagaccgga tcgggtgacc cgccaataat tttaatccca ggacgtctgc aaactcgctg
    11761 gtaacccggt tggtcttgat catcaaacca tcctgttccc gcgtatcctt taaaatattc
    11821 ctgagggttc tgccgctgcc cccgttgccc ggccagatct gggccctgca tacgctgacg
    11881 acggaactca gattctggta ataatcccgg agggcctgga tttgttccgt gttttccgct
    11941 gcggctgtcg taatggctac gacctgctcc ggtttatatc ccaggttttt ttgacggata
    12001 aattccagtt gtttgtaaaa taaaagcgcg cctacgatga ggatgaggga agcggagaat
    12061 tgcacgacca ccagcgtacg cctgaataat ccggctcccg tattttttct gaaagaggtt
    12121 tgaagcaggg attttgcccg gaacgaagaa agataaaagg cgggatagga cccggcaaca
    12181 agtaccaggc aaagcgtgat agcggcggta atgagcagga gatcgggcga caggagggag
    12241 ccggccgata aatttttctg ggcgatgcgg ttgagcaggg gcaggccggc cgcaaccatt
    12301 ccgcaggcca ccagaaccga taccccggtg accaaggcag tctcaaggta aaaccgccct
    12361 gcgagttgga accatccggc gccaatggtt ttattgatgc ccacttcccg gaaacggagt
    12421 tgtgattttg ccgtagccag attcatatag ttcatagaag cgatcagcag gaccaccagg
    12481 gccaggatac ccaggatttt tacctgcccg ggatcgccgg tacgggtggt ggatgcattg
    12541 gtaatgcccg cggatcccag atggatgttc ttcaggggct gcagccagat ggtaaaccaa
    12601 cggttgtcgg cttcgacctt tgactccagc aaccgcaaca tgctttgttc aaccttttct
    12661 tccagggcac cggtttcaag caaaacataa gtttcaaaac tcgcattgct ccagaccagg
    12721 tttttactgg cccattctat actgctgaag gaaccgatca gggcgggatc cagggtggaa
    12781 ttgcccggaa aatcggaata gacgcccgtg atttcgagat cgtatttatt gtccaccttc
    12841 aacacttgtc ccaggggatc ttccgcgccg aaatatttat cggccaggct ggaggagatc
    12901 accacggtat ttggccgatc cagagccgtt gaagggtcac ctttcaaaaa aggaaggtca
    12961 aaaatcctga acaggcccgg gtcggaccag tacagttttt tctccacaaa ttttttttga
    13021 ccgctggtga tgaaagcagt tttgccataa tcgtgataaa ggagccgggc ttgctccgcc
    13081 acttcgggga tttccgcctt catggcaggg ccaacggcat tgggggtgtt ggcccagtcc
    13141 atgttcttgc cgtcaaaggt ggcggtcagc cctacccggt aaatccggtc cgattgggta
    13201 tggtaacggt caaatgattt ttcatccttc acatagatca gtaacagcag ggcaataccc
    13261 aggccgctgg acaggccgag tatattcaaa aaagtgaaca ggcggttttt aaataaactg
    13321 cgccaggtga ctttcagata attgcggatc agcatgatat ctcgttttgg tccgggaccg
    13381 cccggtcctg aatgaattct ttttgctttt tgtgaggagc ccgggacttt tcccggtttc
    13441 caattagtgc gttctccttt tcctattact caccgcccgg atattacccg aaggttaatc
    13501 ttgagtcatt ccgttcgtaa acttttgacc gggttcatca gcgccgcttt taaagcctgg
    13561 aaacttatgg taaaaagggc aatcaccaaa gccgccagtc ctgcaattac aaatacccat
    13621 ccgctgatgt ttatcctgaa agcgaagtcc tgcagccacc ggttcatcag gaaccagccg
    13681 agcggaaagg ccacgacggc cgcaatcaaa accaggcgga taaagtccct ggacaacatg
    13741 gtgatgatat ccggcacctc tgcgcccagg actttacgga tcccgatttc tttggtacgc
    13801 tgttctgcga taaaagtggc caagccgaaa aggcccaggc aggcaataaa gatggccagg
    13861 atcgagaaag acagtgcgat cttccctact ctttgttccg cacggtacat ttcatcgaaa
    13921 gactgatcga ggaatcggta ggaaaagggg gcccctttag ccatcgttct ccaggtatct
    13981 tcgatttgcc cgatgagttg ctgaatattg cccgtattca ctttaaaggc ggtggtccca
    14041 tccgcttttc ccaggaaaaa acacaaagga ccgactttct gccgaaggga ttcaaaatta
    14101 aaatccttta ctacgccaat cacatggaag gcttccacct gattttgttc aatcctggat
    14161 ccgccataga ttttctggtc caggggattg gattcaaatc cgagcagcct cacggcttct
    14221 tcattgagga tgatggcgga agaatccgta cccctttctt tcgagaaatt tcttccggcc
    14281 aggatttcca tacccatcaa gggaatgtag tcctcatcga ttctccaaac ctgcatgttc
    14341 agtccccggt ttacatccgg cacggcatcc ttgaaaaaag tattgtcgct cctcgaactg
    14401 ttggacaccg gaaggtaacc ggcaaaggtg cttgcggtaa ccccgctcag tttggagatt
    14461 tcatttttaa atgcttccgt attgggccgg agggcgccgg tgccgtcgat gaccaggacc
    14521 tggtccttat taaaaccgat gtttttgctt tgaatgtatt tcagctggtt gtacaccacg
    14581 atggccccga tgatgaggag gatcgaggtg gcaaactgga atacgaccag ggctgaacgc
    14641 aagccgcttt tattgagtga tccctccatt ttgcctttga gcacggcgat gggcctgaaa
    14701 gccgacaaat agaaggccgg gtaaatcccg gctattaaac caaggaggaa gggcaggctc
    14761 aggtaaacgg ccagccatcc cggtttgaac aattccttca tgccaaaggt cttggcggac
    14821 aaatcgttga aaaaaggcag gaggagcaaa gccattccaa tgcccgccgc cgtggcaaaa
    14881 aaggcgatca gggtggactc ggtaaggaac tggctgatga ggttgttccg tcccgtgccc
    14941 agcactttac ggacccccac ttccctggcc cggttggcgg aacgggcggt ggaaaggttc
    15001 ataaaattga tgcaggcgat gagcaggata aacaaggcca ccgctgaaaa aatatacaca
    15061 tactggatgt tgctgttggc ggcaagttca gcaacccggt ttgagtacag gtggattttg
    15121 gtgaccggga acaggaaata ttcgagttta tttcccgccg ctttaaattc atccatgctt
    15181 tttatgggag cgaattgctg cacctggggg atgacgtact tttccagaaa acccgggaag
    15241 tttttattga attcaagcgg gtcagcgcct tcctgcagcc ggatgtaggt cgtaaaattg
    15301 ttgctcaggt aactgcccca gttgtaatcc acattgtcta tggaaaagaa caagtccttg
    15361 tcaaaatggg tattgcgcgg gacatcccgg ataacggccg taacggtgta aaacttgtcg
    15421 cggtcgtccg tctccagggt ttttccgatc acctcttcaa ggcccgtatt cccgaaatat
    15481 ttcctggccg cggtttcgga aaggatgacc ttgcccggtc catgcagcgg ccgctgggca
    15541 tcgcccgcca ggacttcaaa cgggaaaatc cgcagcaggg tggaatccgc atggaccacc
    15601 ccgccctctt caatgtactc gctgcccttt ttgatcagtt tggacccgct ggatgcgtat
    15661 aaccggacga attcctccac ctggggataa tcctgtttca gggtcggacc catcggatcg
    15721 gagctcaccg cgaggttcag ttcacttccc cctattctga tatcggcatc cacccggtac
    15781 agccggtccg catatttatt ccaccggtcg taggacactt catcgacgat gtaattggcg
    15841 atcagtataa aacaggcaat gccaatggcc agcccggtca catttaaaat ggaaaaggtt
    15901 ttgtttttga tgaggttgcg ccaggcggtg aggaggtagt ttttatacat gattcaattt
    15961 tttggagaaa aggaggcggg aggaagggag gaaagaagat ccctactcca ccagtctttt
    16021 ctattcattt aaatttttct tttctcacct ttattaatcc atacaacatt gaagaaattt
    16081 ttttcgtttt ttcaagcatt gctacagact tctcttgatt caaaatccct atgcgcattg
    16141 ccaagtgaac ctgggtcctt aattcaccac aggatccctt ggcgatatat aggaactgga
    16201 taaattcctt gttggtctgt ctttcaaaac cttcggctat attcgaggaa atggaaaccg
    16261 ctgaacgttg cagttggtcc ctgaaactat aatcccggct tgctttcaat tccagataaa
    16321 tctcttttgc aagttccaaa gcttcgttcc aaacagccag atcttcaaac ttttcaattt
    16381 tcatagagtg ttttattgtg tggttactgt aatcgcttcc cttctccctt cctcctgtct
    16441 cccttcctcc tgtctccctt cctccctttc tcctaaacca gtatattctc catcaccgtc
    16501 cttccgtcga gcatccggac gatccggtgg gaataccggg cgtcgtgttc actgtgggtc
    16561 accatgatga tggtcgtgcc ctgttcgttg aggtcggtga gcaattgcat gacttcgttg
    16621 ccgttggagg agtcgaggtt tccggtcggt tcgtcggcga ggatgagttt cggtttgttg
    16681 acgacggccc tggctacggc gacccgttgc tgctgtcctc cggagagctg ctgcggaaaa
    16741 tggttgcggc ggtgcatgat ctgcatttta tccagcactt cctccacccg gcgcttccgc
    16801 tcatcggatt ttaccccggt atagatcagg ggcagttcca cattttcgaa gacgctgagt
    16861 tcatcgatca ggttgaagct ctggaacaca aagccgatgt tgtgcttgcg cagatcagcc
    16921 cgtttccttt cattgaattt ggccacttcg atgccgtcaa agaggtagga acccgaatcc
    16981 atgtcatcca gcaatcccag gatattgagc aaggtggact tgccgcaacc gctgggtccc
    17041 atgatggcga tgaattcgcc ggttttcaca tcaaacgaca ccttgttgag ggcaatggtt
    17101 tccacctcct cggtgcggta ggttttttct aggtcagtta ttttgatcat aatttaaagg
    17161 ttagaggtta gaggttagag gttagagtat gccactcaag tctaacttca ctaccctggg
    17221 taataatttt atcagtggat attgtttctg ttttattatt tcaggcactt ttgcccaaat
    17281 tttatatttt caattctcac acatgaaaca ctatgagaaa tttttacgac ttaaaggttt
    17341 ggaaactatc ccatgaattg actttaaaaa tttacgggac cgttatttca tttccaccag
    17401 aagaattatt tggcctcaga agtcaaatga ggcggtcctc ttcttccatt cctaccaacc
    17461 ttgcagaagg ttgcggccga aattccaatc ctgattttaa aagatttgtc aatattgcta
    17521 tcgggtcaac ttccgaactt gaataccagc tacttctagc caaggattta aaatatctta
    17581 acgaaccgac atttatcgaa ctcactaata atgttataga aattcgaaga atgctaatct
    17641 ctctatataa aaaagcccct ctctaacctc tccccgctca cctcttacct cttacctcta
    17701 acctctaacc tcctacctct aacctcctac ctctaacctc ctacctctgc aacaccaact
    17761 cctccatctt cccatacgtc tcataactgt tcgtgaccac cttgtccccg ggctttaatc
    17821 cgcttaatac ttcataatgg tttggatttt gccgtccgag ggtgatgtcc tgtttgaagg
    17881 ccaggttgcc ggcctcgttt actttaaaaa tccagttgcc gccggtggtt tggaaaaagc
    17941 cgcctttggg gaccaggatc accacggatt cgtcgccgag ggccaggccg acctgcaggg
    18001 tctgtccacg gcgaatgccc tggggtacct cgccggtaaa ttccatatcg acctggaaac
    18061 gaccgccggt cacctgcgta tagatttttt tgatcttgag gaaatacttc ttgccggcaa
    18121 aggtgaagtc gccttcaagg ccggtgaata cgcgggagat gtagtgttcg tctatatccg
    18181 cgcggatctt atagccgctg agcacgtcca gctggccgag ccgttcgccc ttctgtttgg
    18241 attgaccgat ttccgcgtcc aggctggtca gctggccatc caccggggcg cggacgatga
    18301 ggtcaccaac cttccggcgc atcagctgca gggcattttg catgcgcgca taggattcct
    18361 ccatctgggc cagctgggtg cgcgtggaaa ggctgtcctg ggtcaggatt tgttccgtca
    18421 ggttcttccg ccggacctgg tagttgtaaa ggttttccga tgatttaaaa tcctggaagc
    18481 cgatggcttt ctgttcgtaa agttttttat tcagatgata gacccggctg gcttcttcga
    18541 gggcattggt cacctccgcc atctggttga gttgccggat cgtattctgt tctgcattgt
    18601 ttttggtatt ctgcatctgg gtgaggacct cgaataccgc cgtttcctgg ttggccaggg
    18661 tcagttcgag gtcggtattg ctgaggcgaa ggatgggctg gccgcgttta agcatggctc
    18721 cgtcttcgac caatttctct tccacacgcc cgccttccag ggcatccagg tatatggtgg
    18781 tgatgggcat caccaccccg ttgatgggaa tgaactcttt aaagggccct tcaaccactt
    18841 cactgatcat cagcctttcc gtatccacat tcagtttgga tttgccggaa gaagatcgca
    18901 ccgccatgta tatgatcacg cccagggcaa ggaggcccaa aatgctgaga attctttgtg
    18961 tcgtccaggt ttttttcgct attggtctgt ccaccgtatt agtttatttt ctgctcttaa
    19021 taaatacgga agatcgtgcc aaaggttaca gactattgat tatcaatact atacaaaaat
    19081 gttatttaat aaaaactgtc cgcttttgat acaaaagtgt gcggaaatgc gttttgccgg
    19141 agatgcaacc aggggaaaat aataccgtct tttaggagta taaccatatg ccgatgacgg
    19201 ataaccggat tttcccgaaa tttggaaaat atatgaacct ccgcgaaagc agtatactta
    19261 tagtggatga tgatccggat gtactgactg cagtccgtct tcttttaaag cccctggtcc
    19321 gttccatatc tttcagccgc tatccggatg aaatcaaatc cctgcttaag gaacaggatt
    19381 tcgacattat ccttttggac atgaatttcc gcagcggcat ccattccggc aacgagggtt
    19441 tgttctggct gaaagaaatc aaaaaaataa aaccccaggt catggtcatc cttattaccg
    19501 cttacgggga catcgacctg gccgtgcgtt ccctcaaaga aggcgcggaa gactttatcc
    19561 tcaagccctg gcaaaatgaa aaactgatca acagcctcca tgccgcgttc aaaaaacaag
    19621 tcaaaagcgc accggctccg ccggatgcca tccgcctcct gggcgagtcg gagaaaatga
    19681 aagacctgat ggtcaaggta tccaaagtgg cgcccaccga tgccaatgtc ttgattcttg
    19741 gagaaaacgg taccggcaaa gacgtgctgg cccaccaaat ccattaccat tcgtcccggg
    19801 ccgacaaacc ttttatcaag gtggacctcg gtgcacttac cgaatccttg tttgaaagtg
    19861 aattgttcgg acataaaaaa ggttctttca ccgatgcccg cgaagaccgg gtcggccgga
    19921 tagaagccgc ccagggcggg accctcttcc tggatgagat cggaaacatc aacctgaccc
    19981 agcaagccaa actgctcacc gtcatccaaa accgccacat cacccggatc gggtccaaca
    20041 tacccagcca ggtggacatc cggctcatct gcgcgaccaa cctcccgatg aacgaactcg
    20101 ccaacgaaca gcggttcagg cgagacctgc tgtaccggat caataccgtg gacctgcacc
    20161 ttcccccgct ccgagaacgg gacaaggata ttgaaattct cgccaaatat tttctccaga
    20221 cctattccgc caaatattta aaaccggacc tcaaactcgg ccgcggcgcc ctggaagccc
    20281 tcctgtctta tccttttccc ggaaatatcc gcgaactcca gtacaccatc gaacgggccg
    20341 tcattctctg cgacaaagac gaactgagcg cttccgaaat cattttttcc ggtattgaat
    20401 cctcccaaaa actcgccgac gcgggagatt tcaaactgag cagcgtggaa aaaaacacgg
    20461 tgatcaaagt tctggaaaaa cacagcggca acatcactaa agcagccaag gaactcggca
    20521 tcacccgcac cgcgctttac cggaggctga ataagtatga gatttaggtg ggaggtaaga
    20581 ggtaagagga ggtctcaact agctaacttg ctaacttact aactggttaa ctgtttaaca
    20641 gattgctatt tttaccccat ggcatttgaa cgctttgaat ggaagatcat acggagggtg
    20701 gtcattttac tggttacggc gctggccttt acctggttgc tggtgaacag ctattacatc
    20761 tatatgctga taccaggagt cgtgcttggc tacctggctt acgaaatgta tgtgttcaac
    20821 cggaaagctt atgatgaaat cgaccagtat gtcgaatcgg tccattaccg ggatttttcc
    20881 aggcatttta atgtgaagga agcgcccaag gaactcaaat cactccggga aggattcaac
    20941 gagatcaacg acaccatcaa ggatatctcc cttgaaaagg aaacccatta tcaatacctt
    21001 cagaaagtcc tggagatcat caacaccggc atcatctcct acgaagagca aggcgggcaa
    21061 gtcatctggc tcaatgaaac cctcaaaaaa atgctccagg tgccttacct gcggaccatt
    21121 cattccctgg aaaaaagaaa ccccctgctc taccggaaaa tcatcgatat caattcgggc
    21181 aaccaggata tcgtcaaact gagcgaaggc ctcaaagaac tcaaggtcct gctcaattcc
    21241 accgctttcc tgctggaagg aaaaaaatac aaactcgtcg ccattcagaa catcaacgaa
    21301 gcgatcgacg agaccgaagc gaatgcctgg caaaaactgc tcagcgtaat gacgcacgaa
    21361 atcatgaaca gcgtggcccc catcgcctcc ctggccgata ccctcaagac ccggctgcag
    21421 gatgccactc cggatgaaat ggaggacctg ggcgttggca tcgaaaccat taaaaaaaga
    21481 agcgaagggc tgctccgttt tgcccagacc taccgtaacc tcaacaaagt cagccagccc
    21541 cagatcaagg ccatcccgct cggagaattg ttcagcacca tgtacaacct gatgaacccg
    21601 accctggaac aaaaaaacat tgatctggat atcatcctta aagaccccaa cctggtcctt
    21661 catgccgatc ccaacctgat cgaacaggtg atcatcaacc tcctgctgaa tgccaaggac
    21721 gccctcctcg ataaaccgga cgcccgcatc atgttgagcg cggaagaatc caacaaccgg
    21781 ctggtcctca aggttgcaga caatggccgg ggcatcccgg cggaagtggc cgataaaata
    21841 tttgtaccct ttttcagcac caagaccaat ggaagcggca tcggcctgag cctttcaaaa
    21901 cagatcatgc tcatgcacaa gggaaacatc cattatgcct cgagggaaaa cgaagggacg
    21961 gtctttgaac ttgtatttcc caaactcccc gcctgacccg gtatttattc cacttccacg
    22021 agaaaggtct tgctgaccaa ccggtgcaag cggtcatatc caaccagttt gatttcaaac
    22081 aagccggtca gatcgccggt atggaagctt atcctgacct ggccgtcctc attggctttc
    22141 acgtttgcat cccagtacaa gagggatcgc aagtcaggtt cacggtggtc cgaaggcggg
    22201 cggtagatcc agggcgatcc ggttgaccag ccctggacag gcaaggccaa acatccatat
    22261 cgggccgcgt gatctttcaa ttgggcaccc tgaatggcaa gcagcccgct gtgtttcatc
    22321 aaggtatcca attgagaaag aatagtggat tccttgttaa aaatcccaat gctgttgacc
    22381 tgggagacct cctgttcaaa taccgcggat tctttacccg caaaaagtcc gtccaccatg
    22441 atccatgccg gccaccggaa cagttccttt ttgatcctgt ttctaaggcg cagtttgttg
    22501 cggtcgtcca ttacggcaga cgaccgggca tccagcatga cttcacggat aaaatcgccc
    22561 accgtatgga tttgctgaaa atctttgagc tggtagaccc ggtcaggaac caggctcttg
    22621 aatgattcca ccggcgggcc ggcctgttct ccgggaattt tgtaaaaatc atggacaatc
    22681 cttcttttgt aattcaattg aaggtaggcc tggacagatg gagccgggac agggaagcct
    22741 tcaattgtat tcaggggcaa ggcgggatta aaatcatccg gcttgatctc caggggacgg
    22801 ctttgattgg gattgagcga gagcgcctgt acggcctgca ctccttcaaa agccggcaac
    22861 ttcagtttga accgcccgct atcggctgaa aaacgctgaa aagaaccaag gtggggaaca
    22921 aacaaggaaa gatatttttc tgagaccagg ctctgatcaa ccggatcgat caactggcct
    22981 tccagcacag gctctgtccg cagttcgtca atgccaggat ttatatccgg gctcctgtta
    23041 aattgcatca gccgggtggg gtccggggat ccgggaagat attgaaaaac ggataccgat
    23101 ccgttttgaa agataccacc cgcgggattg gagaccgtat tcaggtccag ttgtaccctg
    23161 ctcctgcgtt ttacacgggc atatatctct ctgccgtcgg aaacaaaagt gggtccgggt
    23221 cttccaaggt cggttatttc ttcttcaaag tcgttgtaga cgtcgaccgg taggaagccg
    23281 gattcaaatt ttggtaccgt gccgaacctg gaatcccaga ccaggtagaa atgcaggaaa
    23341 taacggccgg tttccagttt agacggaagg tagatacatc cctccgctcc cagggaatcc
    23401 agctttaaca attgcctgtc cagggtgaat tgttttgcat tgagtaaacg cacataaagc
    23461 aaacccgggt ttttatccag cttgtcgaga taatagattt tgaatctcag cgcgtcaccg
    23521 gagatataga cgctccggtc caggtgtatg ccgaatccct ggcaataccc ttcccggcct
    23581 gcgaagagcc atgacatcaa aaagataagg tgccggattt tgtttttcac caccagtccg
    23641 gtttatcgat gtgcgggaat ttgatcgaaa aacaattgca gcagccttca aagtaccc
//
LOCUS       DAOQXT010000075        23430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2532_length_23430_cov_5.528513, whole genome shotgun sequence.
ACCESSION   DAOQXT010000075 DAOQXT010000000
VERSION     DAOQXT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23430
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2532_length_23430_cov_5.528513"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="PKM27_13070"
     CDS             complement(<1..1799)
                     /locus_tag="PKM27_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR08243.1"
                     /translation="MIRHYFKLARRGLLKHKYYTLINVFGLVAGVLSALIITKYLGGA
                     FQFDRHHEHKDRIYALSQEESVNDGIKIKSEATYQGLGDQVRQYPEVLQVARYNHHLE
                     SLVLADLDSGDRRSYHEKNIFYADSGFLKIFSFPLRYGDRGTALSRLHSIVLSGPASR
                     RYFGAQNPLGKTLTIRVPWGAETSYEVTGVLEDIPQKTQFQFDFLISLGATDPNESWI
                     VPDGSTFVLLKNRADIGALSAKMTTALHELPPLKSTHRKVWVSMDPLTKVQWSGMQYL
                     LMAAGLFILIISWVNYINQVIAQSYWRMKETGVFQVLGATRLNLKKQFMVESGLIGLI
                     SLVLVLVLYRLFEPALQSFTNGQILPLIGDPTPINVVFLGIFIGGSLLAAAIPAIILF
                     SSRLGPGLRNIDHNRFGSTSIRQGLVVLQFSMSTILMISVLVISSQLKYMKDKDKGME
                     MENVLIVRAPIIKDTTWVEKRKTVERFKKQCEGLPFVLQAVSSTTVPSEEYRQETYLS
                     LQGSEAKYMVHQNGVDDRFFDLYAIKFAAGGPFIPNANHKNRNSIILNESAARGLGLY
                     DFANRINTRIVDHESGEVYDLIGIVRDYHQTSL"
     gene            complement(2088..2360)
                     /locus_tag="PKM27_13075"
     CDS             complement(2088..2360)
                     /locus_tag="PKM27_13075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNR08244.1"
                     /translation="MNFEFTQNAWDDFEYWMETDRVMLDKIKELLNDIKKSPFQGLGK
                     PEPLKYDLKGYWSRRISGEHRLVYKVDGKKRENQKCTILQCRFHYE"
     gene            complement(2357..2623)
                     /locus_tag="PKM27_13080"
     CDS             complement(2357..2623)
                     /locus_tag="PKM27_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNR08245.1"
                     /translation="MQAVSISQLRSNIKKYLDAVSQSVDIIIVPRNNEEDAVVIMSIK
                     EYNTLLETGHLLSTNANRVRLQESLDQAKTGKTIKFNLSDDLAK"
     gene            complement(3284..6592)
                     /locus_tag="PKM27_13085"
     CDS             complement(3284..6592)
                     /locus_tag="PKM27_13085"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HNR08246.1"
                     /translation="MNKALLCLAFLSLCFSTAFAKIIVVKNEGDAFSPAVIFATVRDT
                     IEFQLATSHNVVEVSKPVWDANGSVSNGGFLLPFGGGQLTVTTVGTYYFVCEPHAIFG
                     MKGILHIAEAGEKYIARLSGMQEEFPFAGLGTGEVTANLLDDTLTVSGSFKNLTGNFN
                     PNVAGGAHIHRGLAGTSGGIVLPLKSIVDTALKSGVFPADSNRFILTAAQKEQLQGRE
                     FYVNIHTTLISSGEIRGQLLPQSEFYYQANLFGSQEVPQVMTTAHGAIVAELSGSNLV
                     ITGSAKDLSSGINTGIRMGGHLHLGYPGQNGGIQIELTPTLDTSGTSVQYLAANNTFS
                     LNVNQLAALRNQQLYANIHTNQYPGGEIRGQLTPMSTAKFRVHLSGAYEANPVNTLAS
                     GGLIINLIDSTITVAGSFSGLESNLAVNVSGGSHLHRGMAGQNGGIVQAIKATLDASQ
                     RAGLFIPDSNTYKLTPVLLDDLLSRRLYANIHSLNFTGGEIRGQVIPECGNVMYGFFT
                     GMQENKPVQSTGRGAVVAEVNGTRLTVSGYAERLLDKVNRNIGSHLHRGQTGANGPIV
                     IPLSMSFPTADSLSLVYPAAQNMYTVSNAFLDSLRRRQIYANIHTLRVGSGEVRAQLN
                     HEALAYFFTRLSGASENPPVRTTGAGALFAEFGGGTTRNIIVHGSFSGLEGKYAANVA
                     GGSHVHTGIAGSNGGIRQGLTVSLGPDSLSGAFLPGNNVYNIGDGGLDSLRKRLFYAN
                     VHTTKAMGGEIRGNFMPLANSYFAANLSPINEVPPLGGTAAGAVKAELLGDRLTVTGS
                     FANLNGDFNRNVAGGAHIHTGATGTNGGIAVGLKAETNPDNKGGRFFSDSNSVTLTAG
                     QIVDLHAGNLYVNVHTTTNAGGEIRGQLLFEPNAFPTAPVISSPAPGDTIRTGLKPDS
                     TYSITWSAGTDPDNNPLVYLIQASIVPEFTLILNTGLVGSNTAFTATIGSIDSTLALL
                     GIPPGGSVTLQFRVLASDGSLSTPGPVIPLTFQRGISTPVVDEFLRSYSMTLFPVPAT
                     QFALLELNAAKSSPMDLQVHEMNGRLAYREKIQVQNGHNRFRIDVSKFSPGMHILSLA
                     VEGRQVASMKLIKE"
     gene            6726..7346
                     /locus_tag="PKM27_13090"
     CDS             6726..7346
                     /locus_tag="PKM27_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNR08247.1"
                     /translation="MKKTVVFDLGGVLIDWNPRYLYRDLFEQEKEMEDFLANICTNEW
                     NELQDAGRSLEEATEVLCTHFPEYDSLIRQYYGQWQRMLKGPIPGTVNILSTLKENQY
                     RLLALTNWSNETFPVALELYDFLHWFEGILVSGKEKMKKPDPAIFNLLIRRYGLNPAE
                     TIYIDDSAKNVQAAIRAGFDTVHFRDPEDLLAGLADRGVMVGLGAH"
     gene            complement(7669..9090)
                     /locus_tag="PKM27_13095"
     CDS             complement(7669..9090)
                     /locus_tag="PKM27_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNR08248.1"
                     /translation="MADPKGFLKYDRELPPSRPIEERIRDYREIYVEEPEEHTRQQAA
                     RCMDCGVPFCHSGCPLGNIIPDFNDAVYREQWDEAFHILRSTNNFPEFTGRICPAPCE
                     ASCVLGLHSSPVTIEHIEKSIAEKAYEEGYVKINKPAHRTHKRVAVIGSGPSGLAAAD
                     QLNQYGHEVTVYERNDKPGGLLRYGIPDFKLEKWVLDRRLKYMEEEGVHFRVNANVGF
                     GIDARELLESHDALVLCGGSTIPRDLPIPGRNLKGIHFAMEYLEQCNRRNAGMTIDPA
                     SEINPSGRKVLVIGGGDTGSDCVGNSVRLGASQVTQIELLSKPPLERTATNPWPLWPM
                     ILRTSSSHEEGCERHWAILSKEFVEGEAGRVNGLKAVEVEWKSDPHTGQNKMLEIPGT
                     EKILECDFVFLAMGYLHPQYEGVLQTLDIAVDSRGNVSDKNYQTSHPKVFSAGDMRRG
                     QSLVVWAIAEGRQAAEAVNQYLS"
     gene            complement(9096..13595)
                     /gene="gltB"
                     /locus_tag="PKM27_13100"
     CDS             complement(9096..13595)
                     /gene="gltB"
                     /locus_tag="PKM27_13100"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNR08249.1"
                     /translation="MDNSDQKGLYVPQLEKDSCGVGLFANLNGDKVHRVVDQALTMLE
                     NMEHRGACGCEPETGDGAGILVQTPDRFFTKVCRDLGFTLPAFGQYGVLMCFLPKDDQ
                     LFNKTMDQARRLIAHSHFHILGQRDVPVNNFGLGKSALSTEPRMVQLFVAPNAPLLVR
                     ELERQLYVLRKYIIHSVYKILPEVEDSFYISSSSYKTIIYKGQLTTHQLRSYFPDLQH
                     ADFASAIAIIHSRFSTNTVPKWKLAQPFRYIAHNGEINTIMGNLNWWRAKEDHLQSEI
                     FTAEDNLMLKPIPWRGLSDSGSFDNVLEYLVQSGRSVPHSLMMMIPEAWEHDEQMAQY
                     KKDFYAYHQNLMEPWDGPASMCFSDGIVVGATLDRNGLRPSRYSLTEDNELVVASETG
                     VVDLDQAKIILKGRLQPGRILIADLDQHRIVGDEEVKKVVCQNKPYGDWLARRRKRIE
                     DLGKPMPFEPLSAKGPSLVERQQWAGMTKEDLKVILQPMVMEERDPLGSMGTDTPLAA
                     LSDKPQHLGNYFKQLFAQVTNPPIDSLRERSVMAFTSRLGASGNILEADESLAAFIYM
                     EHPVMTHSEMNLLKSVSNPDFKLCTLSTTFALTGPFSLENAVKALCHLAEVKVREGFN
                     ILVLSDADIVQGKAAIPSLMATGAIHHHLIQTALRSKTSLVVQAADIWESHHFALLFG
                     YGANAIHPYLALETVWFREQETLPEDYKTIEKKFIKGLEKSLLKIMAKNGISTLQSYQ
                     GAQIFEAVGIHDSVIETCFRGTVSRIGGLNFEGIQKEIGLRYQIQPKGELRDAGIYQW
                     KRNGEFHLFNPQSIHLLQMSTQLNDYSTFKKYSKVIDDQDQQACTLRGLFHFKKGNPI
                     PIEEVEPIENILKRFSTGAMSFGSISYEAHTTLAIAMNRIGAKSNSGEGGEDPKRYLI
                     QENGDYLRSAIKQVASGRFGATSYYLSEADELQIKMAQGAKPGEGGELPGHKVNDWIG
                     RVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANRNARISVKLVSKAGVGIIASG
                     VAKAHADVILISGHDGGTGASPLTSIRHAGLPWELGLAESHQTLLKNKLRDRVILQCD
                     GQLRSGKDLVTATLLGAEEWGIATAALVVEGCILMRKCHLNTCPVGIATQDPELRKKF
                     HGKPEHVISFFTFLAREMREIMASLGFRTINEMVGRTDLLEWKPNQTWKTQYLDLDPI
                     LQYQAPEEGTGTFKSVPQDHGIDDILDRKLIEHAKPALESGQHITGIFNIKNTDRATG
                     TMLSYEISRKWHGEGLPPATIRYRFRGSAGQSFAAFGARGLHFILEGESNDYFGKGLS
                     GATLVVSPDRKNKFDPSANIIVGNVALYGATSGEVYIKGMAGERFAVRNSGASAVVEG
                     VGDHGCEYMTGGRVLILGKVGKNFAAGMSGGIAYVKSTWENLDKWCNKEMVELEPPAE
                     EDFEEIRKLLRNHFHYTSSRIALDLLENWELKKDEFIKVMPVDYKRVLLEKKKQIAEF
                     VTVDK"
     gene            13792..15339
                     /locus_tag="PKM27_13105"
     CDS             13792..15339
                     /locus_tag="PKM27_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08250.1"
                     /translation="MPIAKSDHLIELIHSLSKAEKRHFKLFSSRLQRQEGTQFIKLFD
                     ILEKSKDIEDDFIRKKLGGIAGVKYANLKRNLYSNVLTSLRLIHSSKKVDLEIREQID
                     YARILHGKGLYLQALRLLERIKQVAKNHDQEILLLEIIEFEKLIETSHLTREHKDRTK
                     QLTDEADQLSGIVGQSIKLSNLKILIYGKYVQMGHVQNNKDEKAIQVYFKSHLPKVNE
                     SHLSTLDAITYHQCMVWYYFILLDFRQCMEHAVIWRDLFVSKSFMIGRDPDSYMHAMH
                     YILTCSYNLKQYKVFKQALEDLEKFRKDNYQRFNENSRLLSFIYVHTSRFHLYFLEGR
                     FREGLDIVPKTLRRLDKYKEKLDEHRILVIYFKIAWMYIGAGDPRTASNYLDRIIDLK
                     SVSLREDLQGYARLLNLIAHYEMGNKGLLSYLVRSTNYFLKKMHDLNKMQKATLHFFN
                     RLMNADAFKEKELFASFYPELVTLSKDHYEQQAFIYLDIISWVKSKVQHQTLQQVVAQ
                     SFAETNK"
     gene            15354..16769
                     /locus_tag="PKM27_13110"
     CDS             15354..16769
                     /locus_tag="PKM27_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNR08251.1"
                     /translation="MENNKTSLQRSLGLLSCIVLVISAIIGSGVFKKVAPMSDSLHLP
                     AFVLLAWILAGTITLFGALSNAEIAGMFAESGGEYAYFKKIYNPFFAFLYGWGCFTAI
                     RSASIASIAYVFAESLNHLIRLPAIGNSSMNLFGMFNVLDNLTVKLIATVLVVGLSYL
                     NFKGLKLGENVSRTLTYTLVAAIVFLIISGLMSSVGSGSNLTQPSASYIAEPFFSGHF
                     LALMFTAMLGAFWAYEGWNNLGYLGGEIKNPQRNIPLALLFGLLIVMTLYVLLNLVYH
                     YISPIDAIIARQTTGSGIAAVDIVGLYLGRAGTLFVSCLILVTTFNATNATILMSSRV
                     YYAMARDGAFFKKAEYVHPEYKTPSYSLWMQCGWSVLLIFSGSFDQLTDMLIFASFLF
                     YGATALGVFIMRKKAPNLERPYKVIGYPIVPGIFVLFCLGLLIYTPSQRPMEALAGIL
                     LILTGIPFYWFWKKRGVVVGG"
     gene            16855..17649
                     /gene="proC"
                     /locus_tag="PKM27_13115"
     CDS             16855..17649
                     /gene="proC"
                     /locus_tag="PKM27_13115"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNR08252.1"
                     /translation="MNKISIIGGGNLGTAIAEGLLASGFVKAKDLIVTRRNPNALQQL
                     AEQGVSILSDNAKAIKSSEVVILAVKPYQVPVLLKSVASAFTPGKHLLISVVTGLETR
                     TIREIVKKKISIIRAMPNTAIAIRQSMTCISGDGATAAQLNYCQTLFEQLGKVTVIDE
                     ALMEAATALGACGTAYAMRYIRANIQAGIEIGFNSEVASLIAAQTVKGAAELLLTRDT
                     HPESEIDKVTTPKGCTIAGLNEMEHQGFSSSLIRGVVTSYRMIAKD"
     gene            17890..17961
                     /locus_tag="PKM27_13120"
     tRNA            17890..17961
                     /locus_tag="PKM27_13120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17923..17925,aa:Arg,seq:ccg)
     gene            complement(18026..18649)
                     /locus_tag="PKM27_13125"
     CDS             complement(18026..18649)
                     /locus_tag="PKM27_13125"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNR08253.1"
                     /translation="MKHLNSRLEAFCDGVFAIAMTLLIIDIKLPESDIQTTGQLWHAI
                     LGITPSIASFLLSFIVVLITWINHHNHMQLIGKPSGSFLYANGFLLLTVVFIPFPTSL
                     LGEFLFTDHASPAVILYNAVLALQAIGWILISRTAIRNQLCNGEKAVGQIRLNGRFGV
                     FAFALYSLCAVLATWFPLLIAAFTTITWIFWLIFGIRIKQEGEQLLP"
     gene            18980..19297
                     /locus_tag="PKM27_13130"
     CDS             18980..19297
                     /locus_tag="PKM27_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR08254.1"
                     /translation="MNLRRDVFQAIADPTRRAILLLVASHSMTAGAIASHFETARPTV
                     SKHLQILTECELLEPEQNGREIYYHFNPKKIKEIADFIEPFLKLWDDRFNKLEAIMKK
                     YPR"
     gene            19322..19789
                     /locus_tag="PKM27_13135"
     CDS             19322..19789
                     /locus_tag="PKM27_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNR08255.1"
                     /translation="MERKTKVIAGEGSQEIWITREFDLPLNLLFKAYAEPGIVEQWMG
                     TKVLKLENRRHGGYQFETSDAQGNVVFRANGTIHEFDPDRKITRTFEMENTPFPVQLE
                     FLEFEKITEETSRLNMQIVYKSVALRDQMLRLPFAQGINMAHNRLQEVVNKLK"
     gene            19795..20214
                     /locus_tag="PKM27_13140"
     CDS             19795..20214
                     /locus_tag="PKM27_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNR08256.1"
                     /translation="MKKRDKIIYWVSTLWLALGMVSTGLVQLFRVEAPGAVAPPGVYG
                     IQYLGYPVYFLTILGAWKLLGVIAVLLPGLPLLKEWAYAGFFFLMSGAVFSHFALKDP
                     WIEFLPSLLLLALTVSSWYFRPSERKIMTQPAGQNFA"
     gene            20233..20799
                     /locus_tag="PKM27_13145"
     CDS             20233..20799
                     /locus_tag="PKM27_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNR08257.1"
                     /translation="MKNQPKKLSAEQVKDLLSTLGTRFEKNPGRHAGLEWAPIEARLK
                     AKPQKLGSLHEMESTGGEPDVVGLDKKTGEYLFYDCSPESPKGRRSLCYDQRALESRK
                     EFKPADSAMNLASAMGIELLTEEEYRALQKLGNFDAKTSSWIITPPDIRRLGGALFGD
                     YRYGTVFVYHNGAESYYAARGFRGVLRV"
     gene            complement(20849..21541)
                     /locus_tag="PKM27_13150"
     CDS             complement(20849..21541)
                     /locus_tag="PKM27_13150"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNR08258.1"
                     /translation="MNTKITFLAQVLATLWLIPSAFAQSTYRIGGLPSLNLNLKLNND
                     WSLNTKLESRQQFRSGEWNGKAIDQYEYLLTDLSLIAAKKIGLNSRLAGGYLVRSGEG
                     EWFHRFIQQFVVVQKLSGFRLAHRILSDQTFSKHEKPGFRFRYRISSEIPLNGESVDE
                     GELYLKINNEYINSLQAMEYDLEIRLTPLLGYDITESFKIETGPDYRINSFINGNTRH
                     SIWMMLNFFIEI"
     gene            complement(21531..22103)
                     /locus_tag="PKM27_13155"
     CDS             complement(21531..22103)
                     /locus_tag="PKM27_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08259.1"
                     /translation="MTTWFRILLLVIPLSGNIRAQEKFEKESRIGEKEVPTQALLFLA
                     PLNLSKVKWYKEEGLNGTSIEVKFKAGKARYSVEFDTLGRVEDIEIEADWDDLASGLR
                     DSIAASLNNHCIQHKVIKVQRHFKGDEAELFSIIRDGKNIPSLTHQYELIVRCNQEHQ
                     VDQFEYLFSDTGQPVSRSKIVFRNSSHLEY"
     gene            complement(22232..22486)
                     /locus_tag="PKM27_13160"
     CDS             complement(22232..22486)
                     /locus_tag="PKM27_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNR08260.1"
                     /translation="MDGKSLITTLVLGAVIGWLAGQVKRGFGFGLLGNIIVGILGSLL
                     GSWLFGLLGISLGSGLLGTILTGVIGAVVLLAVIGVIKRS"
     gene            complement(22498..>23430)
                     /locus_tag="PKM27_13165"
     CDS             complement(22498..>23430)
                     /locus_tag="PKM27_13165"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNR08261.1"
                     /translation="NWSPFMPNGVGGILKGTAAVFFAYIGFDALSTMAEECKNPQRDL
                     PRSMIYSLVICTVIYVLIALVLTGMTHYTRLDVGDPMAFVFNEISLDWLAGVIAVSAV
                     IATASVLLVFQVGQPRIWMSMSRDGLLPPVFSKIHPKYKTPSFSTILAGVVVAVPALF
                     LNLDVVISLTSIGTLFAFVLVCGGVLVLSGDPNRIQPKFKIPYLNAKYVMPFIGFAIL
                     WLLGIKIAHMYKETGFWHALPYLIYLALTVWVTVLSVLRNYSLIPVLGLLSCSYLLCE
                     SGTSNWIAFLVWLAVGQLLYFVYSYRHSKLKVTN"
BASE COUNT         5566 a         5923 c         6309 g         5632 t
ORIGIN      
        1 ttgagggagg tctggtgata gtccctgacg atcccgatca gatcatagac ttccccggat
       61 tcatgatcca caatccgggt attgatccta ttggcaaaat catagaggcc caggcccctg
      121 gccgccgatt cgttgagtat gatgctgttc ctgtttttgt ggtttgcatt ggggataaaa
      181 gggcccccgg ctgcaaattt tatagcgtag aggtcaaaaa accggtcgtc caccccgttt
      241 tgatggacca tgtatttggc ttcgctgccc tggaggctga gataggtttc ctgcctgtat
      301 tcctcactgg gcacggtcgt ggacgagacg gcctgcagga caaagggaag cccttcacat
      361 tgctttttaa accgctccac cgtcttcctt ttttccaccc aggttgtgtc ttttatgatc
      421 ggcgcccgca cgatgagtac attttccatc tccatgccct tgtccttgtc tttcatgtat
      481 ttcaactggc tggaaatcac caggacgctg atcatcagga tggtggacat ggaaaactgt
      541 agcaccacca ggccttgccg gatgcttgtg cttccaaacc ggttgtggtc gatgtttcgc
      601 aagcccggcc ccaaccggga tgaaaacagg atgatggccg ggatggctgc cgcgaggagg
      661 ctgcccccta taaaaatccc caggaaaacg acattgatgg gtgtgggatc cccgatcaag
      721 ggcaggatct gaccgttggt aaaggattgc agggccggtt cgaaaagccg gtaaaggacc
      781 aggaccagga ccagggagat caggccgatg aggccggatt ccaccataaa ttgttttttc
      841 agattcagcc gcgtggcacc gagcacctgg aacacgcccg tttccttcat cctccagtag
      901 gattgcgcga tgacctggtt gatataattg acccaggaaa tgatcaggat aaaaaggccc
      961 gcggccatca gcagatattg catgccggac cattgtactt tggtgagggg gtccatcgat
     1021 acccacactt tgcgatgggt ggattttaag ggcggcagtt catgtaaagc ggtggtcatc
     1081 ttggcggaaa gcgcgccgat atcggcccgg tttttcagga gcacgaaagt ggagccgtcg
     1141 ggcacgatcc aggattcatt gggatccgtg gcgcccaggc tgatcaaaaa atcaaactga
     1201 aattgggttt tttgagggat atcctccaac actcccgtca cttcatagct ggtttccgcg
     1261 ccccagggaa cccttatcgt cagcgttttt cccagcggat tctgcgcacc aaaataacgc
     1321 cgggaagccg gtccggagag gacgattgaa tggagcctgg acaaggcggt cccgcggtcg
     1381 ccataacgaa gtggaaaact gaagatttta aggaaacccg aatcggcata gaatatattc
     1441 ttttcatggt aggatcgccg gtcgcccgaa tccaaatctg cgaggactaa agactccaga
     1501 tggtggttgt agcgcgccac ttgcaggact tccgggtatt ggcggacctg gtccccaagc
     1561 ccctggtagg tggcctcgct ttttatttta atcccgtcgt ttacggattc ctcctggctg
     1621 agggcataga tccggtcttt gtgttcgtga tgacggtcaa attgaaaagc acccccgagg
     1681 tatttagtga tgatcagggc cgaaagcacc cctgcaacca gtccgaagac attgatcagg
     1741 gtgtagtatt tatgtttcag gaggcctctc cgggcgagtt tgaagtagtg gcggatcatg
     1801 ggatgtgtgt gatggggcga attaggtatt tatattattt ccaaggtacg tatagatata
     1861 attttatatt ggaaattctt cctttaattc cttctgcgtt cccggtcttg attccgcgtg
     1921 agcggaagcg ggtttataat tggctgatct atctatttaa aaggccaagc atgccaaggt
     1981 tgttcaagtg caattgacac ctggggtctt ttgttctgtg cattccgctt cacctaagcc
     2041 gacttttacc tggtttctcg gactgcccct ggtctttagg gcaaagatta ctcataatga
     2101 aagcggcatt gtaggatggt gcatttttga ttttcccttt tttttccatc tactttatac
     2161 accagtctgt gttcgcctga aattcttctt gaccaataac cctttaaatc atacttcaaa
     2221 ggttcaggtt tacccaatcc ctgaaagggg gattttttaa tgtcgttaag aagttctttg
     2281 attttgtcca gcattacgcg atcggtttcc atccagtatt caaagtcatc ccaggcattt
     2341 tgtgtaaact caaagttcat tttgccaggt catccgataa attaaatttt atggttttac
     2401 cggtcttagc ctggtccagg gattcttgca atctgaccct gttggcattg gtcgacaaca
     2461 agtgccccgt ctccagtaat gtgttatact ccttgatgga catgatgacc acggcatcct
     2521 cttcattatt cctgggaact ataatgatgt ccacggattg ggagaccgca tccaggtatt
     2581 tttttatgtt tgatctcaac tgggaaatgg aaactgcttg catagtttta aatttgtaca
     2641 aataaaagta caggattttg tacgaaaact agagtggaaa agacaggagg gtggcacata
     2701 atcccgtcca agcgggcttc caggtatagt tatcaatcag aaaagacctg ctcccgcagg
     2761 tgtgggaatg accagcgccc ggggaaccaa ggactaaaag aaaaaaggac catcatataa
     2821 aatatctaca acacccctgt cccccctgac taccattttc aaatactacc cttggggcag
     2881 gcccccgaca actaatcctc aaatcctaca gttagggcaa gccacggggg aaccaaagat
     2941 cttggcagta tactcaaaaa aaacaatatt ccttgccctt gggaaggagt acagaaatgg
     3001 atcaatcaaa atgcggagaa ctactgcaaa aatggatata aaatatttac aacacccctg
     3061 tcccccctcc tgactacaat tctcaaatgc cacctggggg cagacccacg ggggaaccaa
     3121 ggtgcctggc agttgaataa aataaaacaa tcttccttgc ccttaggaag gagtacagaa
     3181 atggatcaat caaaatgcgg agaactccta caaaagagga ctttcatata aaatatcttt
     3241 aactcctctg tccccctttt aacctctgac ctctgacctc accttattcc ttaatcaatt
     3301 tcatactcgc aacctgcctc ccttccaccg ccagggaaag gatgtgcatg ccgggggaga
     3361 atttgctcac gtcgatgcgg aagcggttgt gcccgttctg tacctggatc ttttcccggt
     3421 aggccagacg tccattcatt tcatggacct gcagatccat gggggaggac tttgccgcat
     3481 tcaattccag gagggcgaat tgggtggccg gcaccgggaa aagcgtcatc gaataagacc
     3541 tcaaaaattc atcgaccacg ggggtggata tcccacgctg gaaggtgaga ggtatgacgg
     3601 gaccgggggt ggacaaactg ccgtcggaag cgaggacccg gaattgcagg gtcacggagc
     3661 ccccgggcgg aatgcccagc agggccaggg tgctgtcaat ggatcctata gttgctgtaa
     3721 aagccgtatt actgcctacc agacctgtat tgaggatcag ggtgaattcc gggacaatgg
     3781 aggcctggat caaataaacc agtggattgt tgtccggatc ggtccctgcg gaccaggtga
     3841 tggaataggt ggaatcgggt ttgagtccgg tccgaatggt atccccggga gcagggctgg
     3901 atatgaccgg ggccgtcgga aaagcattgg gctcgaataa caactggccc ctgatttcac
     3961 cgcctgcatt ggtggtagta tgtacgttga catataaatt gccagcatgc aggtccacga
     4021 tctggcctgc cgtgagggtg accgaattgg aatctgaaaa gaagcgccct cctttattat
     4081 ccggattggt ttcggctttg aggccgacgg caatgcctcc gttggtccca gtggccccgg
     4141 tatggatatg cgctcctccg gcgacattgc ggttgaaatc cccgttgagg ttggcaaagg
     4201 aaccggtgac cgtcaggcgg tctccgagca attccgcttt tacggcaccc gcggccgtac
     4261 ctccaagtgg aggcacttca ttgatcgggc tcaggttggc cgcgaaataa ctgttggcca
     4321 ggggcataaa attgccgcgg atctccccac ccatggcttt ggtggtatgg acattggcat
     4381 agaataatcg ctttcgcagg gaatccaggc caccgtcgcc gatattgtaa acattattgc
     4441 cgggcaggaa agccccgctc agactgtctg gtccaaggct aaccgtaagt ccctgccgga
     4501 tccccccatt gcttccggcg atcccggtgt gcacatggga tccaccggct acattggccg
     4561 catatttgcc ttccagtccg ctgaaagaac catgcacgat gatattccgg gtggtccctc
     4621 cgccaaattc ggcgaataac gctcctgcac ccgttgtgcg aacaggtggg ttttcgctgg
     4681 cgccgctgag ccgtgtgaaa aaataggcaa gggcctcatg atttaattgg gcgcgcactt
     4741 cgcctgagcc caccctcaag gtatggatat tggcatagat ctgacgcctc cggagggaat
     4801 ccaggaaggc attggatacg gtgtacatgt tctgagccgc gggatagacc aggctgagac
     4861 tgtcggccgt ggggaaactc atggacaatg gaatcacaat cgggccgttg gcgcctgtct
     4921 ggccccggtg caggtgtgaa ccgatattgc ggtttacttt gtccagcaga cgctcggcat
     4981 agccggaaac cgtcaggcgg gtcccattga cttcggcgac cacggcgccc ctgccggtgg
     5041 attgcaccgg tttattttcc tgcatgccgg tgaaaaatcc gtacataacg tttccgcatt
     5101 cggggatgac ctgtccccgg atttcccctc ctgtaaaatt gagggaatgg atattggcat
     5161 aaagccgcct ggacaataaa tcatccagca ggaccggagt cagcttgtag gtattggagt
     5221 ccggtataaa caagcccgct ctctggctgg catccagggt ggcttttatg gcctggacaa
     5281 tgccaccatt ttgaccggcc atgccccggt ggaggtggct tcctcccgat acattgacgg
     5341 caagattgct ttccagaccg gagaaactgc cggcaaccgt gatggtggag tcgatcaggt
     5401 tgatgatcag gcctccgctg gcgagggtat tgaccggatt ggcttcatag gccccggaca
     5461 gatggaccct gaatttggcg gtagacatgg gcgtgagctg gccgcgaatt tcgcctccgg
     5521 ggtattgatt ggtgtggata ttggcataca actgctggtt gcgcagggcg gccagttggt
     5581 ttacattcag cgaaaaagtg ttgttagccg caaggtattg gacactggtg ccgctggtgt
     5641 ccagggtggg ggtcaactcg atttggatac ctccgttctg gcccgggtag cccaagtgaa
     5701 gatgcccgcc catccggatg cccgtattga tcccactgct caagtcctta gccgaaccgg
     5761 tgatcaccag gttggatccg ctgagttccg ccacgattgc tccatgtgct gtggtcatga
     5821 cctgaggtac ttcctggctg ccgaaaagat tggcctggta ataaaattca gactggggca
     5881 atagctggcc acggatttca ccagaggaaa tcagggtggt atggatgttt acataaaatt
     5941 ccctgccttg caattgctct ttctgagcgg cagtgagaat aaagcggttg gaatccgcgg
     6001 gaaaaacgcc ggatttcaag gcggtgtcga cgatggattt gaggggaagc acgatgcccc
     6061 cgctggtccc ggccaggccc cggtggatat gggcgccgcc ggctacatta ggattaaagt
     6121 tcccggtgag gttcttaaaa ctcccgctca cggtgagcgt atcatcaaga aggttggcgg
     6181 tgacctctcc ggtacccagt ccggcgaagg ggaattcttc ctgcataccc gagagccggg
     6241 caatatattt ttcccccgcc tcggcgatgt ggagaatgcc tttcatcccg aaaatggcat
     6301 ggggttcgca tacaaaataa taggtgccga cggtggtgac ggtcagttgg ccccctccaa
     6361 agggtaggag aaaaccaccg ttggaaaccg aaccattggc atcccagacc ggtttggaca
     6421 cttctaccac gttgtgggaa gtggccagtt ggaattcgat ggtatccctg acggtggcga
     6481 agatgaccgc gggagagaag gcatcgcctt catttttgac cacgatgatt ttggcaaaag
     6541 cggtggaaaa gcataaggat agaaaggcca ggcagaggag tgctttattc atatgatgag
     6601 gtttaatatt atgttaacga ttcagggacc caaaaatata cctgccaggt cttaaaattt
     6661 tgaactgatc caaataaaaa gaggtttatc gcaggtacaa ttttattatt ttgcctttta
     6721 atagcatgaa aaagaccgtg gtttttgatc tgggcggggt gttgatcgac tggaatccaa
     6781 gatatctgta ccgggacctg tttgagcagg aaaaggagat ggaagatttc ctcgccaata
     6841 tttgcaccaa tgaatggaat gaattgcagg atgccgggcg ttcgctggag gaagccacgg
     6901 aggtcttgtg cacgcatttc cccgaatacg attccctgat ccgtcaatat tatgggcagt
     6961 ggcagagaat gctcaagggc cctattcccg ggacggtcaa catactcagt accctcaagg
     7021 aaaatcagta ccggcttttg gccctcacca actggtccaa cgaaaccttt ccggtagccc
     7081 tggaactcta cgatttcctg cactggtttg aaggcatcct ggtgtcgggg aaggagaaga
     7141 tgaagaagcc ggatcccgcg atcttcaacc tgctgatccg gaggtatggt ttaaatcccg
     7201 cagagacgat ttatattgat gacagtgcga aaaatgtgca ggcggcgata cgggcgggtt
     7261 ttgatacggt gcatttcagg gatcccgaag atttgttggc ggggctggcg gatcgggggg
     7321 tgatggttgg gctgggggct cattgagtgt tcgctggggt ggttccccgc aagggcacgg
     7381 tagtcgtttc ccacaaagac gcagcgaagc gaaggacacg gttttattcc cacaaagacg
     7441 cagcgaagca aaggacacgg tttcattccc gcaaagacgc agcgattgtt cccacaacga
     7501 cacaaaggac acggttttat tcccacaaag acgcaaagaa cacagttcaa ttcccacaac
     7561 ggcacaaagg gtttcccacg cggacacaaa gggcataaat gatctccctc aatgaggtga
     7621 agacacgaag tacctgccaa cttgctaact taccaactta ctaacttact aacttaaata
     7681 ctgatttacc gcttccgcag cctgccggcc ttcagcaatg gcccagacca ccaggctctg
     7741 gccacgacgc atatccccgg ctgaaaatac tttaggatgg gaagtctggt aatttttgtc
     7801 cgaaacattg cctcttgaat ccaccgctat gtccagcgtt tgcaataccc cctcgtattg
     7861 gggatggagg tagcccatgg ccaggaatac aaaatcgcat tcgaggattt tttcggtgcc
     7921 cgggatctca agcattttat tttggccggt gtggggatct gatttccatt ccacttccac
     7981 cgctttcagt ccattgaccc ttcctgcttc cccctctaca aattctttgc tgaggatggc
     8041 ccagtgacgc tcacagcctt cttcatggga ggaggaggtc cgcaggatca tgggccacag
     8101 cggccaggga ttggtggcgg tacgctccag gggcggtttg gaaagcagct cgatttgggt
     8161 cacctggctg gctcccagtc gtacgctgtt gcccacgcag tccgatccgg tatccccgcc
     8221 tccgatcacc aggaccttgc ggccggaggg attgatctcg gaagccgggt cgatggtcat
     8281 acccgcattg cggcggttgc attgttcgag atattccatg gcgaagtgga tgcctttcag
     8341 attccggcca gggatgggca ggtcgcgggg gatggtggag cctccgcaaa ggacgagggc
     8401 gtcgtgggat tccagcaatt cccgggcatc gatgccgaag ccgacgttgg cattgacccg
     8461 gaaatgcacg ccttcttctt ccatatattt cagcctgcgg tcaagcaccc atttctccag
     8521 tttaaaatcg ggaatgccgt aacgcaggag tccgccgggt ttgtcgttcc gctcatagac
     8581 ggtgacttca tggccatatt gattgagctg gtcggccgcg gccagtccag aaggaccgga
     8641 gccgatgacc gccacgcgct tgtgggtccg gtgggccggt ttgttgatct ttacataacc
     8701 ctcttcatac gctttttccg caatgctttt ttcaatatgc tcgatggtca cgggggagga
     8761 atgcaggccc agcacacaac tggcttcaca tggagccgga cagatccggc ccgtgaactc
     8821 cgggaaattg ttggtgcttc tcaggatgtg gaaagcttcg tcccattgct cccggtagac
     8881 ggcgtcatta aaatcgggaa tgatattgcc caggggacaa ccgctgtgac aaaaggggac
     8941 cccgcaatcc atacagcgcg cagcctgttg ccgcgtgtgt tcttcgggtt cctccacata
     9001 gatttccctg taatccctga tgcgctcttc gatcgggcgc gaagggggca attcacggtc
     9061 gtactttaaa aaacctttcg gatcggccat aacaattatt tatcaacggt gacgaattcg
     9121 gcaatctgtt ttttcttttc caggagtacc cgtttgtaat ccacgggcat cactttgatg
     9181 aattcatctt ttttcaattc ccagttttca agcaggtcca gggcaatccg gctggaggta
     9241 tagtggaagt gattgcgcag gagtttgcgg atttcctcaa aatcttcctc cgcgggcggt
     9301 tccagttcca ccatttcctt attgcaccat ttatcgaggt tctcccaggt gcttttcaca
     9361 taggctatgc ccccgctcat gccggcggca aagtttttgc ccaccttgcc aaggatgagc
     9421 acccgccccc cggtcatgta ttcacaacca tggtcaccga ctccttcgac tacggcggag
     9481 gcgccggaat tgcggacagc aaaacgttcc ccggccatgc ccttgatgta aacttccccg
     9541 gaggtggcgc cataaagggc cacgttgccg acgatgatgt tggcggaggg atcaaatttg
     9601 tttttccggt cgggacttac gaccagggtg gcgccggaca ggcctttgcc gaaatagtcg
     9661 ttggattccc cttccaggat aaaatgcaag cctcgtgcgc caaaagcggc aaaactctgg
     9721 cccgcagagc ccctgaaccg gtaccggatc gtggccgggg gcagaccctc cccatgccat
     9781 ttccggctga tttcatagga gagcatggtc ccggtagcgc ggtcggtatt tttaatattg
     9841 aatatgccgg tgatgtgctg gccggattcc agcgcgggtt ttgcatgttc gatgagcttg
     9901 cggtcgagga tgtcgtcgat gccgtgatcc tggggcacgg atttaaaggt gccggtccct
     9961 tcttccgggg cctggtattg aagtatgggg tccaggtcca ggtattgggt cttccaggtt
    10021 tggtttggtt tccattccag caggtccgtc cttcccacca tttcattgat ggtccggaag
    10081 cccagggaag ccatgatctc tctcatttcc cgggcaagga aggtgaagaa ggaaatcaca
    10141 tgttcgggct tgccgtggaa tttttttctc agttcgggat cctgggtggc gatccccacc
    10201 gggcaggtgt tgaggtggca ttttctcatg aggatgcagc cttccaccac cagggcggcc
    10261 gtggcgatgc cccattcttc tgcaccaagc aaggtggcgg tcaccaaatc cttgccggag
    10321 cggagctggc cgtcacattg caggatcacg cggtcgcgca atttgttttt cagcagggtt
    10381 tgatggcttt cagccaggcc aagttcccag ggcaggccgg catggcggat ggaagtcagg
    10441 ggagaggccc cggtgccccc atcgtgtccc gaaatcagga tgacatccgc atgggctttg
    10501 gccaccccgc tggcgatgat gccgaccccg gctttgctca ccagtttcac ggatatcctc
    10561 gcattgcggt tggcattttt taaatcgaag atcagttgag ccaagtcttc gatggaatag
    10621 atatcgtgat gcgggggagg ggagatcagg cccactccgg gcgtggaatg tctcaccctt
    10681 ccgatccaat cgttgacttt atgacccggc aattcccctc cttcacccgg ttttgcgcct
    10741 tgagccatct taatttgaag ttcatcggct tcactgaggt aataggaggt agctccgaag
    10801 cggccggagg caacctgttt gatcgcggaa cggaggtaat ccccgttttc ctggatgagg
    10861 tatcgtttcg gatcttcacc gccttcgccg ctgttgctct tggcgccgat gcggttcatg
    10921 gcgatcgcca gggtcgtatg ggcttcataa ctgatggacc cgaaagacat ggcaccggtg
    10981 gaaaatcgtt tcagtatgtt ttcgatgggt tccacttctt cgatggggat ggggtttccc
    11041 ttcttgaagt gaaaaagtcc tctcagggtg caagcctgct ggtcctggtc atcgatgacc
    11101 ttgctgtatt ttttaaaggt ggaatagtca ttcagttgcg tggacatctg gagcaggtga
    11161 atgctctggg gattaaagag gtggaattct ccgttgcgtt tccactgata gatccccgcg
    11221 tcgcgcaatt cccctttggg ttggatttgg taacggaggc cgatctcttt ctggatgcct
    11281 tcaaagttga ggcctccaat acgggaaacc gtaccccgga aacaggtttc aatgaccgaa
    11341 tcatgaatgc ctacggcctc gaaaatctgg gccccctggt aggattgcag ggtggagatc
    11401 ccgtttttgg ccatgatttt cagcaggctt ttttcaagcc ctttgatgaa ttttttctca
    11461 atggtcttat aatcctccgg cagggtttcc tgttccctga accacacggt ctccagggcc
    11521 aggtagggat gtatcgcgtt tgcgccatat ccaaagagca gggcaaaatg gtgcgactcc
    11581 cagatgtccg ccgcctgtac cacgagggag gttttgctgc ggagggcggt ctggatcagg
    11641 tggtgatgaa tggcgccggt ggccatcagg cttgggatcg cggccttgcc ctggacgata
    11701 tcggcatccg acaagaccag gatgttgaag ccttcccgca ccttgacttc ggccaggtgg
    11761 cagagggcct tgacggcgtt ctcaagggag aaggggccgg tcagggcgaa ggtggtggac
    11821 agggtacaga gcttgaaatc gggattggaa acggatttca ggaggttcat ttcactatgg
    11881 gtcatcaccg ggtgctccat ataaataaaa gcggcaaggc tttcgtccgc ctccaggata
    11941 ttgccggagg cgcccagcct gctggtgaag gccatgacgc ttctttccct cagggaatcg
    12001 atcggggggt tggtcacctg ggcaaaaagt tgtttaaaat aattgccgag gtgctggggt
    12061 ttatccgata aggcggcgag cggggtatcc gtgcccatgg accccagggg atccctctct
    12121 tccatgacca tgggctggag gatcactttg aggtcctctt tggtcatgcc ggcccattgc
    12181 tgcctttcca ccagggacgg cccttttgcg gaaaggggtt cgaagggcat cggttttccc
    12241 aggtcttcta tcctttttct tctccgggcc agccagtccc catagggttt attctggcag
    12301 accacttttt tgacttcttc atcgccgacg attcggtgct gatccaggtc ggcgatcagg
    12361 atccggccgg gttgcagcct tcccttcagg atgattttag cctggtcgag gtccacgacc
    12421 ccggtttccg aggcgaccac cagttcattg tcttccgtca ggctgtagcg gcttggccgg
    12481 agtccgttgc ggtccagcgt ggcgccgact acgatgccgt cgctgaaaca catggaggcc
    12541 gggccatccc agggttccat gaggttttga tggtaagcgt aaaaatcttt tttgtattgg
    12601 gccatctgct cgtcgtgttc ccaggcttcg gggatcatca tcatgaggga gtggggcacg
    12661 gagcgtccgc tttgcaccag gtattccagt acattgtcaa aactgccgct gtccgacaag
    12721 cccctccatg gaatgggttt gagcatgagg ttgtcctcgg cggtgaatat ttcggattgg
    12781 agatggtctt ccttggcgcg ccaccagttg aggttgccca tgatggtatt gatctccccg
    12841 ttatgggcta tgtaccggaa gggttgggcg agtttccatt tggggaccgt attggtcgaa
    12901 aagcggctgt ggatgatggc gatggccgaa gcgaaatccg cgtgctgcag gtcggggaaa
    12961 taggacctga gctgatgggt ggtgagctgg cctttatata tgatggtttt ataggaggag
    13021 gaagagatgt aaaaagagtc ttcgacctcc ggaaggattt tgtaaacgga atggatgatg
    13081 tatttgcgca ggacataaag ctggcgttcg agttcccgta cgaggagggg agcattgggt
    13141 gcgacaaaga gttgtaccat gcgcggttcg gtgctcaggg cggatttgcc caggccgaag
    13201 ttgttgaccg gcacgtcccg ctggcccagg atatgaaaat gggaatgggc gatgaggcgg
    13261 cgggcctggt ccatggtctt gttgaaaagt tggtcgtcct tgggcaaaaa gcacatcagg
    13321 actccatatt gtccaaaagc gggaagagtg aatccgaggt cgcggcaaac cttcgtaaaa
    13381 aaacgatcgg gggtctgcac gaggatcccc gctccgtcgc cggtttcggg ttcacagccg
    13441 caggctcccc ggtgttccat attttcaagc atggtgagcg cctggtcgac gacccggtgc
    13501 actttatccc catttaaatt ggcaaacaat cccaccccgc acgaatcttt ttccaattgc
    13561 ggcacataca ggcctttctg gtcactatta tccatctaac ggttatttgt attattattg
    13621 tgacaatatc gcacatgccc gggcacagcc cgctggcatg aagcggatcg aaaagttatg
    13681 caattcctct ggcctgaccc gttttaatta atggaaaaaa ttgtactcaa caaaaaaaga
    13741 gagactttgt attaaattca atatcaatat gttataacaa tttttcttgt catgccaatc
    13801 gcaaaatcgg accaccttat tgaactcata cactccctct ccaaggcgga aaaaaggcat
    13861 tttaaactct tcagcagccg tctgcagcgg caggaaggca cccagtttat caaactgttt
    13921 gacatcctgg aaaagagcaa ggatatcgag gacgatttca tacgaaagaa gctgggaggg
    13981 attgccggcg tcaaatacgc caatctcaaa cggaatttat attccaatgt cctgaccagc
    14041 ctgcggctga ttcattccag taaaaaagtg gacctggaga tccgcgaaca gatcgattat
    14101 gcgcggatcc tgcacggcaa aggcctttac ctgcaggccc tccgtttgct ggaacgcatc
    14161 aagcaggtgg ccaaaaacca cgaccaggaa atcctcttgc tggagatcat tgaatttgaa
    14221 aaactgattg agaccagcca ccttacccgg gaacataagg accggaccaa acaactgacg
    14281 gacgaggccg accagttgtc gggcatcgtc ggtcaatcca tcaaactgtc caaccttaaa
    14341 atcctcatct acggcaaata cgtacagatg ggccatgtcc agaacaataa ggacgagaaa
    14401 gccatccagg tttacttcaa atcccatctg cccaaggtca atgaaagcca tctcagcacc
    14461 ctggacgcca tcacctacca tcaatgcatg gtttggtatt attttatcct gctggatttc
    14521 cgtcagtgca tggaacatgc cgtgatctgg agagatcttt ttgtcagcaa gtcctttatg
    14581 atcggcaggg acccggattc gtacatgcac gccatgcact acatcctgac ctgcagttat
    14641 aacctcaaac aatacaaggt cttcaaacag gcgctcgaag acctggaaaa attcaggaag
    14701 gacaattacc agcgctttaa tgaaaattcc aggttgttgt cattcattta tgtgcatacc
    14761 tcccggtttc acctgtactt cctggagggt cgcttccggg agggcctgga catcgtgccc
    14821 aaaacgcttc gccggctgga caaatacaag gaaaaactgg acgagcaccg catcctcgtc
    14881 atttatttca aaatagcctg gatgtatatc ggggcgggcg acccgcgcac cgccagcaat
    14941 tacctggacc ggatcatcga cctgaaatca gtgtccctgc gcgaagacct ccagggctac
    15001 gcccgcctgc tcaacctgat cgcgcattat gaaatgggga ataagggatt gttgtcctat
    15061 ttggtgcggt ccaccaacta tttcctcaaa aaaatgcacg acctcaataa aatgcagaag
    15121 gccaccctgc attttttcaa ccgcctgatg aatgcggacg ccttcaagga aaaagagttg
    15181 tttgcctctt tttacccgga actggtcacc ctttccaaag accattatga gcagcaggcc
    15241 tttatctacc tggacatcat cagctgggtg aaaagcaagg tgcagcacca gaccctgcag
    15301 caggtggtcg cccagtcctt cgccgaaaca aacaaataaa tcattgcaag taaatggaaa
    15361 ataacaaaac cagccttcag cgctcgctgg gcttattgtc ttgtattgta ctggtgatca
    15421 gcgcgatcat cggctcgggc gtgtttaaaa aagtggcgcc gatgtcggat tcgctccacc
    15481 tgcccgcttt tgtattgcta gcctggatcc tggcgggaac catcaccctc ttcggagcgc
    15541 tcagcaatgc ggagatcgcc ggcatgtttg cggaaagcgg aggagaatat gcctatttta
    15601 aaaaaatata caatcccttc ttcgcctttc tctacggctg gggctgtttt actgccatcc
    15661 ggtcggcatc catcgcttcg atcgcctacg tctttgcgga gtcgctcaat cacctgatcc
    15721 ggctgccggc cataggcaac agttcgatga accttttcgg catgtttaat gtcctggaca
    15781 acctcacggt aaagctgatt gctaccgttc tggtcgtcgg gctgagttat ctcaacttca
    15841 aaggacttaa attgggtgaa aacgtgtccc gcaccctgac ctacaccctg gtcgccgcca
    15901 tcgtctttct catcatcagc ggcctgatga gcagcgtcgg cagcgggagc aaccttaccc
    15961 agccttccgc ttcctatatc gccgaaccgt ttttttcagg tcatttcctg gccctgatgt
    16021 ttacggccat gctcggcgct ttctgggcct atgaaggatg gaacaacctg ggttatctcg
    16081 gtggagagat caagaatccg cagcgcaaca tcccactggc cctgcttttt ggcctcctca
    16141 tcgtgatgac gctttatgta ttgctcaacc tggtttatca ttatatctcc ccgatcgatg
    16201 ccatcatcgc gaggcagact accggcagcg ggatcgccgc ggtggatatc gtcggtctgt
    16261 acctgggcag ggcggggacc ctttttgttt cctgcctgat cctggtgacg accttcaacg
    16321 ccaccaacgc caccatcctc atgtcctccc gcgtgtatta tgccatggcc cgcgacggcg
    16381 ccttctttaa aaaagcggag tatgtgcatc ccgaatataa gaccccgtcc tattccctct
    16441 ggatgcagtg cggctggtcc gtgcttttga ttttttctgg cagttttgac cagttgaccg
    16501 atatgttgat tttcgcctcc ttcctgtttt acggagccac cgccttaggc gtgttcatca
    16561 tgcggaaaaa agcgcccaac ctggaaaggc cctataaagt catcggttat cccatcgtgc
    16621 caggtatttt tgtattgttc tgcctcggct tgctgattta taccccctcc caaaggccta
    16681 tggaagcgct ggcgggcatc ctgctgattc tgaccggcat tccattttat tggttttgga
    16741 aaaagagggg agtagtggtt ggtgggtagt ggttggcggg ggctctttgg gattaccgca
    16801 cggaatgata ttaataagaa cttcatcttt gcatgaaaat ttgaatattc agtcatgaat
    16861 aaaatatcca tcattggcgg gggcaacctg ggtacggcga tcgcggaagg cctcctggcc
    16921 tccggatttg taaaagccaa ggacctgatc gtcacccggc gcaatccgaa cgccctgcag
    16981 caactggctg agcagggcgt atccatcctc tcggacaatg ccaaagccat caagtcttcg
    17041 gaagtggtga tcctggcggt aaaaccctac caggtcccgg tgcttctaaa atcggtggcg
    17101 tccgccttca cccccggaaa acacctcctg atctccgtgg tcaccggcct ggagacccgg
    17161 accatccgcg agatcgtcaa aaagaaaatt tcgatcatcc gcgccatgcc caataccgcg
    17221 atcgccatcc gtcagagcat gacctgcatc agcggggacg gggccacggc cgcccagctc
    17281 aattattgcc agactctttt tgaacaattg ggcaaggtga ccgtgatcga tgaagccctg
    17341 atggaagcgg ccaccgcgct gggggcctgc ggtacggctt atgccatgcg ttatatccgg
    17401 gccaatatcc aggcgggcat cgagattggc ttcaattcgg aagtggccag cctgattgcg
    17461 gcccagacgg tcaaaggagc ggccgagctc cttctcacgc gggacaccca ccccgagtcg
    17521 gagatcgaca aggtgactac accgaaaggc tgtaccatcg ccggcttgaa tgagatggag
    17581 caccagggat tcagttcttc cctgatccgt ggggtggtga cgagttatag gatgatcgcg
    17641 aaggattgaa aggaagggag aaagggagga aggaacaatg ggcgaaaggc gaagggcgaa
    17701 gggagcaaaa ggataaagaa caaaagtgaa gggcgaaggg tgaagggata atggtgaaag
    17761 gcgaaaggta aagggtgaag ggacaatggt gaaaggcgaa aggtaaaggg tgaagggagc
    17821 ataagttgaa agaggggatg gtcttggaat acttgaaaaa ttgaaggata tttgcgacct
    17881 cattttgaag gcctcgtagt tcaaaggata gaatgggaga ttccggttct ccagataggg
    17941 gttcgattcc cttcgaggct actgatgaac cctctaagta attgatttag agggtttttt
    18001 attttatctt ttttacagct ttcaatcatg gaagcaattg ctccccttct tgtttgatcc
    18061 ggatgccaaa gatcagccag aatatccagg tgatggttgt aaaagcggcg atgagcagcg
    18121 gaaaccaggt ggccaggacg gcgcacaaag aatacagggc aaacgcaaag accccaaacc
    18181 ggccattgag acggatttga cctaccgctt tttcgccatt acataattgg ttgcggatcg
    18241 ctgtccggga gatcaggatc cagcctattg cctgaagggc taaaacggca ttgtacagaa
    18301 tcactgccgg agaggcatgg tccgtgaaca gaaattcacc cagcagcgag gtcggaaagg
    18361 ggataaagac gacggtgagg agcaaaaaac cattggcata aagaaatgaa cccgacggtt
    18421 tgccgatcaa ttgcatatgg ttgtggtgat tgatccaggt gatcaaaacc acaataaaac
    18481 tcagcagaaa actggctatg gaaggggtaa tccccaggat ggcatgccat aactgaccgg
    18541 tggtttgtat atccgattca ggcaatttaa tgtcaataat cagcagggtc atggctattg
    18601 caaatacccc atcgcaaaag gcttccagcc gggaattcaa atgtttcatt atatctattt
    18661 atcccttgat gatttattac atcgctgacc tgagcatgaa ccaataatcc agtttattca
    18721 ttcccccgga ataccaaaca ttgcatcggc tgggttttcc ttaggccctg accccaaact
    18781 gaaccaagat aataaactaa cctgaacctc agatcttaaa gggtattgac aaaattcaag
    18841 ttcacatttt cagataaacg agacttggaa gcctgcctgg cggctgacag gcctacccgg
    18901 ccaagtttgg taaatttttt tgaaaaaaac ttgcgtagtt aaatgactac acatatattt
    18961 gtagtcaaat agctacacaa tgaatctaag acgggacgtt ttccaggcca tcgcggaccc
    19021 tacccggagg gccatcctgc tcttggtggc ttcccattcc atgaccgcgg gggcgatcgc
    19081 ctcacacttc gagaccgcaa ggcccacggt ttcaaaacac ctgcaaatcc tcaccgaatg
    19141 tgaactgctg gaacccgagc aaaacggcag ggaaatctat tatcatttta atcccaaaaa
    19201 aataaaagag atcgcggact tcattgaacc cttcctgaaa ctttgggacg accggttcaa
    19261 taaactggaa gccatcatga aaaaataccc ccggtagttg ccgggaataa cactaaaata
    19321 tatggaacga aaaacaaagg ttatcgccgg tgaaggcagc caggaaatat ggatcaccag
    19381 ggaatttgac ctgcccctca acctgctttt taaagcctac gccgaacccg ggatcgtgga
    19441 gcaatggatg ggtacgaagg tcctgaaact ggaaaacaga aggcacggcg gctaccagtt
    19501 tgaaacctcc gacgcccagg ggaatgttgt gttccgtgcc aacgggacca tccacgagtt
    19561 tgatcctgac cggaaaatca ctcggacctt cgagatggaa aacacgccct tcccggtcca
    19621 gctggagttc ctggaattcg aaaagatcac ggaggagacc agcaggctca atatgcagat
    19681 cgtgtacaaa tcggttgccc tccgggacca gatgctccgg cttccttttg cccagggaat
    19741 caatatggcg cataaccggc ttcaggaagt ggtaaacaaa ctaaaatgac cgatatgaaa
    19801 aagagagata aaatcattta ttgggtatcc accttgtggt tggccctggg tatggtttcg
    19861 accggcttgg tgcaattgtt cagggtggaa gcaccggggg cggtagctcc acccggggta
    19921 tatggaatcc aatatttggg atacccggtc tatttcctga ccattctggg agcatggaaa
    19981 ctattggggg ttatcgccgt tttgctgccc gggttgccct tgttgaagga gtgggcttat
    20041 gcgggctttt tctttctgat gtccggcgcc gtattttcgc actttgccct gaaagatccc
    20101 tggatcgaat tcctgccctc cctcttgctc ctggcgctga cggtgagttc ctggtatttc
    20161 aggccaagcg aaagaaaaat aatgacccag cctgccgggc aaaactttgc ataagatcag
    20221 aatcgcatcg atatgaaaaa tcaacccaaa aaactttccg ccgagcaggt caaagacttg
    20281 ctctccacac tggggacccg gtttgaaaaa aatccgggca ggcatgccgg cctggaatgg
    20341 gccccgatcg aagccaggct caaagccaag ccgcagaaat tggggtccct ccatgaaatg
    20401 gaatccaccg ggggagagcc tgatgtggtg ggcctggata aaaagacggg cgagtacctc
    20461 ttttacgatt gttctccgga aagtccaaag ggccgccgaa gcctctgtta tgaccaaagg
    20521 gcgttggaat ccaggaaaga atttaaaccg gcggacagcg ccatgaacct ggcctctgcc
    20581 atgggcattg aactgttgac ggaggaagaa taccgggccc tgcaaaagct cgggaatttt
    20641 gatgccaaaa catcgagctg gatcatcacc cccccggata tccgccggct cggcggcgct
    20701 ctgtttggtg attaccgcta cggcacggtc ttcgtgtacc acaacggagc tgaatcgtat
    20761 tacgcggcaa gagggttcag gggagtgctg cgggtctgac cctaaggatc aggcccattt
    20821 cgggaagaaa gctgggacgc aggttccctt atatttcgat gaaaaaattc aacatcatcc
    20881 aaatactgtg tcttgtattc ccgtttataa atgaattgat ccggtagtcc ggccctgttt
    20941 caattttaaa actttcggtg atgtcgtagc ctagcagggg cgtaagcctg atttcgaggt
    21001 cgtattccat agcctgcaag ctgtttatat attcattatt tattttgagg taaagttcgc
    21061 cctcgtccac cgattcccca ttcaagggga tctcggaact tatccggtac ctgaatctga
    21121 acccgggttt ttcatgtttt gagaaagtct ggtcgcttag gatccggtgt gccaaacgga
    21181 atccggataa cttttgcacc acgacgaatt gttgtatgaa ccggtgaaac cattcccctt
    21241 ccccggacct gaccaggtag ccgcctgcca gcctggaatt taaacctatt tttttagcgg
    21301 cgatcaggga aaggtccgta agcaggtatt catattggtc gatggctttg ccattccatt
    21361 cgccgctccg gaactgttgc cgggattcca attttgtatt aagagaccag tcgttgttca
    21421 actttaaatt caaatttaag gacggcagac ccccgatacg gtaagtactt tgagcgaatg
    21481 cggaaggaat aagccacagc gtcgccagga cctgggccag gaaggtgatc ttagtattca
    21541 aggtggctgc tgtttcgaaa gacgattttt gacctggaaa ccggttggcc cgtatcggaa
    21601 aacaaatatt cgaactggtc cacctgatgt tcctggttgc accggacgat cagttcatat
    21661 tgatgggtca gggacgggat gtttttacca tccctgatga tcgaaaacag ttcggcttcg
    21721 tcccctttga aatgcctttg cacctttatc accttatgtt gtatgcaatg gttattgagc
    21781 gaggcggcaa tagaatccct gagcccggat gccagatcgt cccaatccgc ttcaatttcg
    21841 atatcctcca cccggcccag ggtgtcaaat tcaacgctgt accttgcttt gccggcttta
    21901 aattttactt caatgcttgt tccgttcagc ccttcttcct tgtaccattt aaccttagaa
    21961 agattcaagg gagctaaaaa caacaaagcc tgggtgggaa cctctttttc accaatgcgg
    22021 ctttcttttt caaatttttc ctgcgcccgg atattgcccg aaaggggaat aaccagcagc
    22081 aggatacgga accacgtagt cattaatgtc tgttttggca tgccgggttt attccgccaa
    22141 aattacgggc gtaacttttg tgatacgaga tgcgagatac gagatgccag atgcgaggta
    22201 cgaggtacga ggtgcgagct tccagcttcg gctaagacct cttgatcacc ccgatcacag
    22261 ccagcaggac cactgcgcca attaccccgg tgaggatcgt ccctaaaagg ccgctgccca
    22321 gggaaatccc gagcaagcca aacaaccagc tccccaggag gcttccgagg atccctacga
    22381 tgatattgcc caataagccg aagccaaatc cccgttttac ctggccggcc agccaaccga
    22441 tgacggcgcc aagcaccagg gtcgtaatta aagatttacc atccatatcg agtagtttta
    22501 attggtgact tttaatttac tgtgccggta actgtaaaca aaatacaaca attgtcccac
    22561 tgccagccaa accaggaagg ctatccagtt gctggtcccg gattcgcaca acaagtagga
    22621 acaggacaaa aggcccagca ccgggatcag ggaataattc ctgagcaccg acaggacggt
    22681 gacccaaacc gtgagggcca ggtaaatcaa atagggcagg gcgtgccaga agccggtttc
    22741 cttatacata tgggcgatct tgatccccag caaccagagg atggcaaagc cgatgaaggg
    22801 catcacatat ttggcattga ggtagggaat cttgaatttg ggctggatgc ggttgggatc
    22861 accggaaagg accagcacgc ctccgcagac cagtacgaaa gcaaacaggg taccaatgct
    22921 cgtcaggctg atcaccacat ccaggttgag gaaaagagcg ggaacggcca cgaccacccc
    22981 ggcaagaatg gttgaaaagg agggagtctt gtatttggga tggattttgg aaaaaaccgg
    23041 aggcaggagg ccatcccggc tcatgctcat ccatatcctc ggttgtccca cctggaagac
    23101 caataatacg ctcgccgtgg cgatcaccgc gctgacggca atcacaccgg ccagccagtc
    23161 gaggctgatt tcgttgaaca caaaggccat cggatccccg acgtccaagc gggtataatg
    23221 ggtcatgccg gtcagcacca gggcgatcag gacatagatg accgtacaga tcacgaggct
    23281 gtagatcatg gaccggggca ggtcgcgctg gggattctta cattcctcgg ccatcgtgga
    23341 gagggcgtca aagccgatgt aagcgaaaaa cacggccgca gtccctttga ggatcccgcc
    23401 gacgccgttg ggcatgaaag gggaccagtt
//
LOCUS       DAOQXT010000076        23037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2573_length_23037_cov_7.433644, whole genome shotgun sequence.
ACCESSION   DAOQXT010000076 DAOQXT010000000
VERSION     DAOQXT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23037
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2573_length_23037_cov_7.433644"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3974
                     /locus_tag="PKM27_13170"
     CDS             <1..3974
                     /locus_tag="PKM27_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNR08262.1"
                     /translation="AAGGSCKITAIYTDTYLDVCGAGFKIFHKWIVTDWCTLEEEVCE
                     QYIKVVDTSAPRILKPLDTIFANVSPHDCVAGVVLPALVPGTDFKDCGTVKQTYTISY
                     DDPSHPGKTVVLSGELPSKTLFLPMGVFTVEINLADACFNRTDTSRGLVITDITPPIP
                     VCNEITQVTIDPATCWAKIAAKELDNGSRDNCCEVLHYAAAHMDSITYWRNYWKEKLT
                     TEVGEKSFAKERLEYEALIEDWINCYVFSDTVNFTDCGSDSIVLRVYEACGVPRYDPH
                     VFPCGPHAWFCYNTYQYIGDFNWNWFDKDGPKSCSYRPALTSLKKLDKKYEGYETKGY
                     LEPKYEGAAQYEYCQVPFYFPSLQLMLQAKSTAGGGNPPGSYCSDRLYNDCMVVVLVD
                     DKEAPVVHELEDVTVYCDASPHYASYPRECSTGKKNGLWPGYLKDSDGTIHGYYGGYG
                     AEDEHAPDCGHYDHKKNWAPVYCREWLYLDSFETGGIIVPETYFEVPVFFDKTRPKRA
                     LAKHEFSITDNCRLDDASLSWTDEGDINSCGEGWIQRTWTMRDACGNEVTARQKVLVK
                     HRSDFEVVFPADTVMVCDYTQGTGPEVTGRPDVTDDECEQITVKFEDQVFSVDDGACY
                     KIVRTWTIFDWCIYEPNRTERYPEVIVDDRLRADTGNRECVYRNLKDNNDGYMQYIQV
                     IKVVDHEGPVIKVKDTVVCITSENCISETVRIPLSATDNCAEPEQIHFQIEIDLNASD
                     AAYADLTYDRSSIEQIVLGNPRELVFAPSGEGRHVVHVIATDNCGNKDTASYRLELKD
                     CKKPTPYCFSGIATVVMPSTGRVTVWAKDLDAGSYDNCTDKGGLIFSFSADQTETSRE
                     FGCADIPNGRSQTIPVEIWVTDEAGNQDHCSTFLLLQDNTGNICPDTAGLNVTIAGTV
                     QTEGKEPVEHVRVDINGTSLDMSYETGSKGSFHFEALPSKTDYTLKANRNDQPMNGIS
                     TLDLILIQKHILGSQPLDSPYKIIAADVDNDKQVTAIDLVELRKLILASYEQLPNTES
                     WKIIPKAQEFSDPQNPWSFKTQMEMKGVEKDYMQEDWIGIKTGDVNASAAAHSLMGTE
                     VRDNNTGLIFEAVDRHFNAGELVTVEFRSPNFRGISGFQGTVEVDGGWWVVDGSHTPG
                     SGALSLSDQNFGTRRIEEGFITMSWNANTGIDVTDKEVLFTMTFKAKQDGRISEALRI
                     GSQLTVAESYEGKGEPGSLGIRFVSPEGNEVSTRSELYQNYPNPFDQRTVIGLRLAKE
                     GRGTLSIHDVTGRTIRSIERDWTRGYHEVWLDRREIGATGVLYYRFESGFFTDSKKMV
                     IVE"
     gene            complement(4129..4998)
                     /locus_tag="PKM27_13175"
     CDS             complement(4129..4998)
                     /locus_tag="PKM27_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR08263.1"
                     /translation="MPSGPDHPVKHNTGSRIKLSVSSYSYWHFKGDKYPIEKVIEQAA
                     ALGLDGIDVLHRQMESEEPAYLQKLKRQAFSSGIALTCLSIHQGFVHPDKEELKKHIQ
                     HTIHCMQLAADMGIPCMRLNTGRWGTIKSFDEFMKNRGQEPALAGYTEEDAFKWCIDS
                     IAECMPVAEKLGVLLALENHWGLGSRPEGMIRIMKAINSPWLQLLMDTGNFLEDPYDK
                     LEMIAPYTCFVQAKTYYGGGEWYSLDLDYPRIIGILRKVHYKGYLSIEFEGKEAADSG
                     VDKSVRLLREILG"
     gene            5180..6700
                     /locus_tag="PKM27_13180"
     CDS             5180..6700
                     /locus_tag="PKM27_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNR08264.1"
                     /translation="MTLTTENILLVGSILLFLSLFAGKTSYKFGVPLLIFFLGIGMLA
                     GSEGIGGIYYDNPKTAQFIGIVALNFILFSGGLDTDWNSIKPVLWQGVTLSTLGVLVT
                     ALSVGLFVHWISDFTLFEGLLLGSIVSSTDSAAVFSVLRSKMLALKGNLRPTLELESG
                     SNDPMAYVLTLSFLGLVIHPESSLLGTIPVFFKQLLLGTLMGFALGRAGKLMINRIRL
                     DYEGLYIVLMIAIMFFSFAATDFVGGNGFLAVYLCAIYLGNQELIHKKKFLKSFDSFA
                     WLMQIVLFLTLGLLVFPRQIVPVIGIGLIISVFLILVARPLSVFLSLYFFRIQTRSKI
                     FISWVGLRGAVPIVFATYPLIAGIDKAPMIFNIVFFISVTSVLIQGTTLSLVARWLGL
                     TLPENLKARTQVDIQLADSIKSVLTEITLPGDNPVIGKQLVQLKLPNTSHISFIERNK
                     KYLTPNGATVLEKNDRLFVLSENKEALKKFCESLEIDPDGLNRKKVEWTGSEPSTA"
     gene            6751..8712
                     /locus_tag="PKM27_13185"
     CDS             6751..8712
                     /locus_tag="PKM27_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNR08265.1"
                     /translation="MKRKSFILPGFLWLMSGFHALAQSPVGFQYVNFSRVEIHDPFWT
                     SRMDKVATVTLPVCIDQTEVKTPRIRNFEAVAAKDGRRHQGIFYDDSDVFKALEAIAY
                     AIKMDKNPGLESKADEWIEKIAAAQLPDGYLNTYYTLNGLDKRWTDMSMHEDYNLGHM
                     IEAGVAYFDATGKRKLLDVCIRMVDHFASLFGPGKRHWVTGHQELELALVKLYHTTSD
                     RKYLDLANWLLEERGHGHASGYTWTEWKDTAYAQDDVPLRKVKDIKGHAVRAMYYYTG
                     AADVAAITGDQEYLEAMKRVWEDVVFRNMYITGGIGASGSNEGFTKDYDLPNDHAYCE
                     TCASVGMVFWNQRMNALTGEAFYADVLERSLFNGALDGISYAGDQFFYGNVLASSGQH
                     KRRDWFGTACCPANIARLLASLGNYIYSSSDRGIWIQQYISSNTVIPLKEVKVPVQVD
                     TKYPYEGKVSIILNNEIPIETNLYLRIPGWARGEAVPGGLYRFLDPAFQWPSITVNGV
                     KTNYTLDKGYAVLHRQWKGGDRIMLDIPMPVQRVVSDSIRYNEGKVALQRGPLVYCVE
                     GADHEGKVFNSILPDRTAVKPVWQPNILGGTLTLEASVPALQVEPDSISVKQQWVRMT
                     AIPYFNWANRGQNEMRVWLPRRFDGLKVD"
     gene            8740..10488
                     /locus_tag="PKM27_13190"
     CDS             8740..10488
                     /locus_tag="PKM27_13190"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNR08266.1"
                     /translation="MNNRVSLLPAFNIETIRGNIPPAFVFFVFFILISEESGAQDSSL
                     FRLAGLIKPVIIHTDRWGVSHIEAQTEEDLFFAQGYQAARDRLFQFELFRRQATGTVS
                     EMLGPRELDRDIGARLFRFRGDITRELNHYHPRGERIIKAFVKGINNCVRDALSREDD
                     LPLEFRLLNIKPGFWTPEDVVSRHQGLLGNVQTELQTARWLRQIGEERLIELMNYHPH
                     SPDLSLDSLLTREALAEDILKWYNAFRRPVRFQKEDLLGMVRPDILSPMPGPDGEIMN
                     YNSEGSNNWVISGNKTQSGYPIMANDPHRAITIPSLRYIVHLKAPGWNVIGGGEPILP
                     GVSIGHNEHGAWGLTIFQTDMEDLYVYRIDPQNRNRYKHHGQWLMFKTIADTVRVKGH
                     SPVKIEHRYSVHGPVVFVDTLRNLAYAVRCAWLETGAAPYLASLRMNQARSWAEFRQA
                     CAYSFVPAENMVWADRKGNIGWQAVGLAPVRKNHSGMVPVIDHVTHEWSGYQPVLERP
                     GAFNPREGFIATANENLTPPAYPYMQSIGYNWADPYRGMRIREVLQGAGDFLSRIWPP
                     CKPIIYPCRPGCWCPS"
     gene            10428..11207
                     /locus_tag="PKM27_13195"
     CDS             10428..11207
                     /locus_tag="PKM27_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNR08267.1"
                     /translation="MAALQTDYLSLPARLLVPLITSTVMTDPQLEKYGQYLASWDYKL
                     DPGSGAAGLYVLTERGIEEKLLALFLPENGDGLRSNDVSLNNLSSQAMVRWLWSPDGR
                     FGADPLEKRNQIIRSSFLEAAEKMISLFGPDFAQWKYGHDKFKHARLQHALSAVVNDS
                     LGTQFNTHSHPRGGYGNTVNSTGDADMQASGASFRIIADCADWDQALAINTAGQQGHP
                     GQPHYKDLFETWARDQYFPLYFSEARIKGVTESVTTLVPLE"
     gene            complement(11228..11851)
                     /locus_tag="PKM27_13200"
     CDS             complement(11228..11851)
                     /locus_tag="PKM27_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNR08268.1"
                     /translation="MFEHIFKNNEAWVARKLALDPAYFEKMAHGQSPEYLYIGCSDSR
                     VTAEELMGVQPGDVFIHRNIANMVISIDLNVMSVINYAVDHLKVRHIIVCGHYGCGGV
                     KASLQSKDLGILNPWLRNIRDVYRLHKDELNAITDPEEKYDRLAELNVQEQCINVIKT
                     AAVQKAYQERGLLVHGWIFDVHTGRLKDLHIDFPRILRSIMEIYRLI"
     gene            complement(11889..12500)
                     /locus_tag="PKM27_13205"
     CDS             complement(11889..12500)
                     /locus_tag="PKM27_13205"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HNR08269.1"
                     /translation="MQETQDIKTENKPNAYYPLSQGESIMGSADESFVPWNVYMTLQS
                     SIQFADYKINLLFVIAGIILTIVIDDSSQLSEEPLSYKITFMLFMLVMIPFIYYSVRT
                     VAAHVTSKPDVPTKKLYFFGDIASMPTSEYIRRFRAYNRAEHYDELLLQIHNLSHIAK
                     LKFYNYGKALYLLCVLMILLVVMLVIQTMQPATAASNALDTVQ"
     gene            complement(12509..13045)
                     /locus_tag="PKM27_13210"
     CDS             complement(12509..13045)
                     /locus_tag="PKM27_13210"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HNR08270.1"
                     /translation="MSKPDINPEDTNILTAYDESFVPWAIYGNLQSSIQFADNKINLL
                     FVIAGIILSIVLESIGDFSEESLVFKIVFVLLLLIMIPFLYYSIRTVAAHTIHRPDVQ
                     TRKLYFFGDILRMPASQFIEHFKSNTRTEHYDELLLQVHNLSHIAKKKFENYSKALYL
                     LSIMMGLLIVLLVSKAFL"
     gene            13259..13561
                     /locus_tag="PKM27_13215"
     CDS             13259..13561
                     /locus_tag="PKM27_13215"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR08271.1"
                     /translation="MTLTQRINPEKLEHAASMLRVLSHPVRLAIVDLLFTNGPLSVQE
                     IQEKLGLEQAIASQHLILMKDKGVLSSVKEGRCHYFSLLYPNMKNIIHCLEECCNA"
     gene            13596..14396
                     /locus_tag="PKM27_13220"
     CDS             13596..14396
                     /locus_tag="PKM27_13220"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNR08272.1"
                     /translation="MHPEAYLLATLIGLSLGLIGGGGSILTVPVLVYVMGISPVLSTA
                     YSLFIVGATSIIGAIQYAQQKLVDFKTGILFCIPSIAAVYLTRKFLIPFIPDPVFTLG
                     QLVISKELLIMLLFAVLMILASVSMILPVREKQYEKILPAWLTLLTVLLEGAGVGVLT
                     GLVGAGGGFLIIPALVLLTRMPMKKAIGTSLLIIALKSLIGFTGDLGHQVLDWNLLVL
                     FTLFAIAGMFLGTALSKRIGGEKLKPAFGWFVLVMGVFIVVNELYLKH"
     gene            14428..15843
                     /locus_tag="PKM27_13225"
     CDS             14428..15843
                     /locus_tag="PKM27_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR08273.1"
                     /translation="MKIEQIYTGCLAQGAYYITSEGEAAIIDPLREVKPYLERLETDK
                     VKLKYIFETHFHADFVSGHIDLSRHTGAPIVYGPDAACEFDCISARDGQEFKIGKVTI
                     KVLHTPGHTLESTTYLLKDETGKDHCIFSGDTLFLGDVGRPDLAQKAAHMTQEQLAGL
                     LYDSLTSKIMPLADDVIVYPAHGAGSACGKNMMKETVDTLGNQKKVNYALNQPNREAF
                     IKAVTEGLQPPPAYFPSNVAMNKKGYQAFEEVMNHGMRALSPAEFEVVAEETGAVVLD
                     TRLPGDFAKGFVPQSVNIGLKGDFAPWVGALLADVNQPILLITEVGQEEEAVTRLSRV
                     GFDHVLGHLAGGYAAWEASGKETDTVNRITAEEFASAFKKEESLVLDIRREGEYEAEH
                     VEDAYSRPLAYINDWIKDINPARHFYMHCASGYRSMIAASILKARGYHNFSEIEGGFT
                     AIKKTEVTCTDYVCQSKIIKQ"
     gene            15856..16176
                     /locus_tag="PKM27_13230"
     CDS             15856..16176
                     /locus_tag="PKM27_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNR08274.1"
                     /translation="MLKFLERLLFGADRGQVKSAIEAGALLVDVRTPSEYASGHVKGS
                     MNIPLESIPKNLSKFKNREPIVVFCRSGARSAQAKVLLNQSGFQHVINGGSWSSVNKL
                     VSNN"
     gene            16188..17390
                     /locus_tag="PKM27_13235"
     CDS             16188..17390
                     /locus_tag="PKM27_13235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNR08275.1"
                     /translation="MKTHYQILVIGGGNAGLSLSAHLLLKNKKLDIGIVEPSDKHYYQ
                     PAWTLVGGGVFDINDTVRNEADFIPKQAAWIKDSCVSFQPEANRVTLASGAVLTYDYL
                     VVCPGIQLDWGSVKGLKEALGQNGVTTNYSFKVAPYTFECLKNFKGGKVLFNNPHTAI
                     KCGGAPHKIMWLAGDYFRIHGLLDKAEIHYYSGGTRLFGVEKYEKTLKKVAERYHVHT
                     HFFEKLEEVDGPNKRARFVGFGEHNKGVETWVDYEIFHVTPPQSAPDFIKQSPLANAA
                     GWVDVDKYSLQHVRYPNVFSLGDSSSLPTSRTGAAVRKQTPVVANNLLSLIGGQTLQA
                     KYSGYSSCPIITGYGKLVLAEFDYNNQPMETFPFDQSKERWSMYQLKKQVLPRMYWNL
                     ILRGRAQG"
     gene            17424..17996
                     /locus_tag="PKM27_13240"
     CDS             17424..17996
                     /locus_tag="PKM27_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNR08276.1"
                     /translation="MNLVETLSQPWPWYIGGPLIGLIVPVLLIAGNKAFGISSSLRHI
                     CAACIPAKIPFFKYDWKKEMWNLFYAGGIILGGLIAAFWLPNPAPVEIHPDLIAELGG
                     YGISQSNSLLPESIFNWNSLLSLKGFIMMIVGGFFVGFGTRYAGGCTSGHSIMGLSTL
                     QWPSLVATCCFMLGGFIMANLILPHLLKMA"
     gene            17998..18522
                     /locus_tag="PKM27_13245"
     CDS             17998..18522
                     /locus_tag="PKM27_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNR08277.1"
                     /translation="METIKTKPEAEQIEKNTDFEVWSLDTAYVNEKDYQQPWWHELKY
                     VLAGIVFGIVLVKTEVISWYRIQEMFRLQSFHMYGVIGSAVAVGALSVFLIKKFRIKT
                     VYKQEIEFHKRPFTKGTIFGGLIFGLGWALTGACPGPLYAQLGSVGTVGIVTLLSAVA
                     GTWVYGKIQDKLPH"
     gene            18544..19527
                     /locus_tag="PKM27_13250"
     CDS             18544..19527
                     /locus_tag="PKM27_13250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNR08278.1"
                     /translation="MKYWETFVNGYTGYANYLWNEILNPSWHNYFYWLIGISLFFWLL
                     EIAFPWRKNQSAIRKDFWLDGFYMFFNFFLFSLIGYNAVSNVFSQAFNDFIRWAFGWE
                     NLVAIRVHQLPWLVQLGILFIVRDFIQWNVHRLLHRSEWLWQFHKVHHSVEEMGFAAH
                     LRYHWMETIVYRTLEYIPLAMIGFGVQDFLVVHIFTLAVGHFNHSNIRVPLGPLKYIF
                     NNPQMHIWHHAEHIPNRYGVNFGLTLSIWDYIFKTNYVPRSGRDEKLGFDQLDAFPKT
                     FLNQFLYPFYKKNIKNEKTNHPAPLAGHSGSSMQRNRTGKIESGPVQFPVG"
     gene            19520..19801
                     /locus_tag="PKM27_13255"
     CDS             19520..19801
                     /locus_tag="PKM27_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNR08279.1"
                     /translation="MAKDKTVQLIDVRTPGEYAGGHIAGSTNIDYNAPDFLDRMRKLD
                     KNKPVALYCAVGGRSGNAAVKLKELGFSKIYDLAGGTQAWAANKLPLVK"
     gene            19828..20964
                     /locus_tag="PKM27_13260"
     CDS             19828..20964
                     /locus_tag="PKM27_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNR08280.1"
                     /translation="MDIPLITVTFIALAGVLYAISQVSFVLRNLHRQPEHQAAFLFAP
                     LNKFRFGLAFLSLGLAFFLGLCWMQGRQAHQKQLLQARSQILAAYDSSKVWQAPDPYL
                     ILKEDHKDLILYGRQLISNTSDFLGPRGTVRRMSNGMNCQNCHLDAGTRPFGNNYSAV
                     LATYPKFRARSASEEDITKRINDCFLRSLNGDSLEAGSREMKAITAYIHWLGQAVPKN
                     HIPLGSGLVKVDLLQRPADPEKGKTVYMAKCQSCHGAEGQGLPKPEDPLSNYPPLWGK
                     GSYNEGAGLYRLSTFAGYVKANMPFGATKDNPQLSDEEAWDLAAFVNSQPRPPHPFLA
                     RDWPVISQKPYDHPFGPFADTFPADQHKYGPYQPIIAFQKSKTK"
     gene            20961..22637
                     /locus_tag="PKM27_13265"
     CDS             20961..22637
                     /locus_tag="PKM27_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNR08281.1"
                     /translation="MKNRRKFLQSLSMGGLGLTMIEPLKPALSASGSQGANPGEAGGT
                     QKITLLQTTDVHCQIHPHDELFWENEKMVFRKTAGYSHISTLFKQYRKSNPNTFIVDT
                     GDMFQGSMLSVKTEGGAYTEILDILAYDLYLPGNWEVIYLKKRMQTLLGGLNAPKVCA
                     NMYHDAGNGAKGELIFPAYHTWTAAGVKIGFLGYTDHLVPIRQSPIYSKGIIYTKPEE
                     NLKYYVDVLKNQEQCDYVIILAHLGLSQQIHIANLPECEGVDYIFGGDTHERVRKPIQ
                     CRYAKVVEPGAFGSFVGKLELTVTNGKIVGDHYELVEVNPKKYKADPAMDRLIDKLED
                     PYEDSIKQVHGYSKVPMYRYFVVENTIDTLIVDALMWKANTDIVLSNGFRFCPPRAAD
                     HTGMVPITEGFVYDMLPVDAVARTAWVTGRQLKEWLERELQNVFASEASKRFGGWVVK
                     MKGMDIEFKAFEEFGKRVQKITIGSEPVDPDKEYSVTACERDGDPMDMLCRIPKVRNP
                     RSLDFTMHQVIRDYVKTFSPVDPTPHHNVKVLDAPQTLLSQVFGVDYKFV"
     gene            22655..>23037
                     /locus_tag="PKM27_13270"
     CDS             22655..>23037
                     /locus_tag="PKM27_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNR08282.1"
                     /translation="MNRKFLLPALFFLGWILPLAAQEPVVNMNEYKLVFQLTNGDTVV
                     HKATVKQIFNALAAAPKSKIELVCHNNGISFLQVSKTQYMGQILELRNKGVVFAACEN
                     TLRERKIDKTDIVPQAVFVPAGIIE"
BASE COUNT         5714 a         5861 c         6067 g         5395 t
ORIGIN      
        1 ccgcggcggg aggttcctgc aagattacgg cgatctatac cgatacttat ctggatgtct
       61 gcggagccgg ctttaagata ttccacaaat ggattgtgac ggactggtgc accctggaag
      121 aggaagtatg cgaacaatac atcaaggtgg tggatacttc ggcgcccagg atcctgaaac
      181 ctttagatac gatttttgct aatgtaagcc cgcacgattg tgtggccggc gtggtattgc
      241 ctgccctagt accaggtaca gactttaaag attgcggaac ggtcaaacaa acctatacta
      301 tcagctatga tgatccctca catccgggta agacagtagt gctcagcgga gaattgcctt
      361 caaagacact ctttctgccc atgggtgtat tcacggtaga gattaacctg gcagatgcct
      421 gtttcaaccg gacggatacg agccggggat tagtcatcac ggacatcacc ccgccgatcc
      481 cggtctgcaa cgaaatcacc caggtgacga tcgacccggc gacctgctgg gctaagatcg
      541 cggccaaaga gctggacaac ggtagccggg acaattgctg cgaggtgctg cattacgcgg
      601 cggcgcacat ggacagcatt acatactgga ggaattactg gaaggaaaaa ctcaccacgg
      661 aagtcggaga gaagtccttt gccaaagagc ggctggaata tgaagccctg atcgaagact
      721 ggatcaattg ttatgtattc agcgatacgg tgaattttac ggactgcggc agtgattcca
      781 tcgtgctgag ggtgtacgaa gcctgtggcg taccgagata cgatccgcat gtcttcccct
      841 gcgggccgca tgcctggttc tgctataata cctaccagta tatcggggat ttcaactgga
      901 actggtttga caaggatggg ccgaagtcat gctcttaccg cccggccttg accagcctga
      961 agaaactgga taaaaaatac gagggatacg aaaccaaagg ctacctggaa ccgaaatacg
     1021 aaggagcggc ccaatatgaa tattgtcagg tacccttcta tttccccagc ctgcagttga
     1081 tgctgcaagc gaagagcacg gccggaggag gcaatccccc gggcagctat tgttcagacc
     1141 ggttgtacaa cgactgcatg gtggtggtgc tggtggacga caaagaggcc ccggtggtgc
     1201 acgaactgga agacgtcacg gtttactgtg atgcctcgcc ccattacgca agctacccca
     1261 gggaatgttc gacgggtaag aaaaacggcc tctggcctgg ttatctcaaa gacagcgacg
     1321 gcacgatcca cggctattat ggcggttatg gcgctgagga cgaacacgct ccggactgtg
     1381 gtcactatga ccacaagaaa aactgggccc cggtatattg cagggaatgg ctctacctgg
     1441 atagttttga aacgggaggc atcatcgttc cggaaacata ttttgaggtg ccggtattct
     1501 ttgacaaaac aaggccgaaa cgggcgctgg cgaagcatga attttcgatc acggacaatt
     1561 gccggctgga cgatgcgagc ctgagttgga cggacgaggg ggacatcaat tcctgcgggg
     1621 aaggctggat ccagcggacc tggacgatgc gcgacgcctg cggcaacgaa gtgaccgcca
     1681 gacaaaaggt attggtgaaa caccggagcg attttgaggt ggtcttcccg gcggatacgg
     1741 tgatggtctg cgattatacc cagggcacgg gcccggaggt gacgggcaga ccggacgtca
     1801 cggatgatga atgtgaacag atcacggtga aatttgagga ccaggtcttc tcagtggatg
     1861 acggggcatg ttacaagatt gtcagaacct ggacaatatt tgactggtgc atttatgagc
     1921 ccaaccggac ggaaagatat cccgaagtga tcgtggatga ccgcttaagg gcagacacag
     1981 gcaaccggga atgcgtgtac cgcaacctga aggacaataa cgacgggtat atgcagtata
     2041 tccaggtgat caaggtggtg gaccatgaag gtccggtgat aaaggtgaaa gacaccgtcg
     2101 tatgcatcac ttctgaaaac tgcatatctg aaaccgtgcg gatcccactg agcgccacgg
     2161 acaattgcgc ggagccggag cagatccatt tccagatcga aatagacctg aatgcaagcg
     2221 atgcggccta tgccgatctg acttatgacc gaagcagcat tgaacagatc gtgctgggca
     2281 atcccaggga actggtattt gctccatcgg gagaaggcag gcacgtggtg catgtcatag
     2341 ccacggacaa ctgcggcaac aaagacacgg ccagttaccg cctggagctg aaggattgca
     2401 aaaaaccgac gccttattgt ttttcgggca tagccacggt ggtaatgccg agtacgggaa
     2461 gagtgacggt atgggcgaaa gacctggatg cgggcagtta cgacaattgc acggacaagg
     2521 gaggtttgat cttcagtttt tccgcggatc aaaccgagac gagcagggag ttcggttgtg
     2581 ccgatatccc gaacggcaga tcacagacga tcccggtaga gatctgggtg acggacgagg
     2641 ccggcaacca ggaccattgc agcaccttcc tgctattgca ggacaatacc ggaaatatat
     2701 gtccggatac cgctggcctg aatgtaacga ttgcgggtac cgtgcagact gaaggcaagg
     2761 aaccggtgga acatgtacgg gttgacatca atgggacaag cctggatatg agttatgaaa
     2821 caggatccaa aggcagcttc cattttgagg ctttgccctc aaaaacggat tataccctga
     2881 aggcgaatcg gaacgatcag ccgatgaacg ggatcagcac cctggacctg attctaattc
     2941 agaaacatat cctgggtagc cagccgctgg attcaccata caaaatcatt gcggcggatg
     3001 tggacaacga taaacaggtg acggcgatcg acctggtaga gttaaggaaa ctgatcctgg
     3061 cttcatacga acaattacca aataccgaaa gctggaagat catccccaaa gcccaggagt
     3121 tcagtgatcc gcagaaccca tggagcttca agacccaaat ggaaatgaag ggcgtggaga
     3181 aagattatat gcaggaagac tggatcggga tcaagacggg ggatgtgaac gccagcgcag
     3241 cggcgcacag cctgatgggg acggaagtga gggataataa taccggattg atttttgaag
     3301 ccgtagaccg gcattttaat gccggtgaac tcgtcaccgt agaattccgg tccccgaatt
     3361 tcagggggat cagtggcttc caggggacgg tggaggtgga tggtggatgg tgggtggtgg
     3421 atggcagcca taccccgggc agcggtgcat tgtccttgag cgatcagaac tttggaaccc
     3481 gcaggattga ggaaggattc atcaccatga gctggaatgc caataccggg atcgatgtca
     3541 cggataagga agttttgttc accatgacct ttaaggcaaa acaggatggc agaataagcg
     3601 aagcattacg tataggttct cagctcacag ttgcagagtc ttatgaagga aagggtgagc
     3661 ccggcagcct cggcatccgg tttgtaagtc ctgaaggcaa tgaggtcagc acaagaagcg
     3721 agttatacca gaattatccc aatccctttg accagcgcac ggtgatcggc ctgaggctgg
     3781 cgaaagaggg ccggggtacg ctgagcatac acgatgtgac cggaaggacc atccgctcca
     3841 tagaaagaga ctggaccagg ggctaccacg aggtctggct tgaccggagg gagatcgggg
     3901 cgactggagt cttgtactac cggtttgaat cgggattctt cacggacagc aagaagatgg
     3961 tgatcgtaga ataatcgttc gtagcggcca gttttaactg gccgcttcgt acaagaatag
     4021 tccttcataa cgacggccta ggtagacgtc gcaacgcagg ccgcccgttg cagccagttt
     4081 taactggcct gtgcgtactc gtacctgagg cttataccgc cattcgattt aaccgaggat
     4141 ttccctcaaa agccgaacgc ttttgtccac acccgaatct gcggcttctt tgccttcaaa
     4201 ttcaatggat aggtatcctt tgtaatggac tttcctcagg atcccgatga tccggggata
     4261 gtccagatcg aggctatacc attctccccc gccataataa gttttagcct gcacaaaaca
     4321 ggtataagga gcgatcattt cgagtttatc gtagggatct tccaggaaat tgcccgtatc
     4381 catcagcaat tgcagccagg ggctgttgat ggccttcatg atccggatca tgccttccgg
     4441 tctggaaccc agaccccaat ggttttcaag ggccagcaag acacccaatt tttcagccac
     4501 gggcatgcat tcggcaatgc tgtcgataca ccatttaaaa gcgtcttctt cggtatagcc
     4561 ggcaagggca ggctcctggc cccggttttt cataaactca tcgaaggatt taatcgtgcc
     4621 ccacctcccg gtattgagac gcatgcaggg tatgcccata tcggcagcca gttgcatgca
     4681 gtgaatagtg tgttggatgt gttttttgag ttcctcctta tccggatgga caaatccctg
     4741 gtgaatggaa aggcaggtca gggcgatgcc gctggagaaa gcttgacgtt ttaatttctg
     4801 caaatacgca ggttcctcgc tttccatctg ccggtgaagg acatcgatgc catccaggcc
     4861 gagcgcagct gcctgctcta tgactttttc aatagggtat ttatcaccct taaaatgcca
     4921 ataggaataa ctcgacactg aaagtttgat tctggatccg gtattatgtt ttaccggatg
     4981 gtctggtccg gagggcattt gccccgggcc aataatcaag ggcaataggc ccccctgaca
     5041 aataaaattt ctcctgttca tgcttccccc gaaattatac aaatttcacc ggctggcata
     5101 caaaatggga atgcaggccc ctaagccctt aatccatcct tcatttaagt tatctttgga
     5161 gtgaagccac ctggaggaaa tgactttaac gaccgagaat atattgctgg taggatccat
     5221 cctgcttttt ctgtcgcttt ttgcaggaaa gacttcctat aaatttgggg tcccattact
     5281 tattttcttc ctcgggattg gcatgttggc cggatcggaa ggcattgggg gcatctatta
     5341 cgacaacccc aaaacggccc agttcatcgg catcgtggcc cttaatttta tccttttttc
     5401 cgggggcctg gataccgact ggaattccat taaaccggta ctctggcagg gggtcaccct
     5461 gtccactttg ggcgtgctgg tcaccgcctt gtcggtgggt ttgtttgttc attggatcag
     5521 tgattttacc ctttttgaag gattgctgct gggatcgatc gtgtcttcca cggactcggc
     5581 ggcggtgttt tcagtcctgc gttccaaaat gctggccctc aaaggcaacc tgaggcctac
     5641 cctggagctc gaaagcggca gcaacgatcc gatggcctat gtgctgacac ttagttttct
     5701 ggggctggtg atccatccgg aaagcagcct gctcggaacg atcccggttt tttttaaaca
     5761 actcctcctg ggcaccctga tgggcttcgc cctcggcagg gcaggcaagc tcatgatcaa
     5821 ccggatcagg ctggactatg agggtcttta catcgtgctg atgatcgcca tcatgttttt
     5881 cagtttcgcc gcgacggatt ttgtcggagg caacggattc ctggcggttt acctgtgcgc
     5941 aatatatctt ggaaaccagg aattgatcca caaaaaaaaa ttcctgaagt ccttcgacag
     6001 ctttgcctgg ctcatgcaga tcgtgctttt cctgaccctt ggactgctgg tttttcccag
     6061 gcagattgtg ccggtgatcg gcattggatt gatcatttcc gtttttctga tcctggtggc
     6121 caggcccctc agtgtatttc tcagccttta ttttttcagg atccagacca ggagcaagat
     6181 ctttatttcc tgggttggcc tccggggagc ggtaccgata gtcttcgcca cctatcccct
     6241 catcgcagga attgataaag cgcccatgat cttcaacatc gtcttcttca tttcggtcac
     6301 ctctgtcctg atccagggca ccaccctctc tttggtggcc aggtggctgg ggctgaccct
     6361 gcctgaaaat ctgaaagccc ggacccaggt ggacatccag cttgccgaca gcatcaaatc
     6421 ggttttgaca gaaatcaccc tccccgggga caacccggtc attggcaagc aactggtcca
     6481 gttgaaattg cccaatacct cccacatctc gtttattgaa agaaataaaa aatacctgac
     6541 tcccaacggg gccacggtat tggaaaaaaa cgaccgcttg tttgtactgt ccgaaaacaa
     6601 agaagcgctg aaaaaattct gtgaaagcct tgaaatagat ccggatggct tgaaccggaa
     6661 gaaggtggaa tggaccggca gtgaaccgtc cacggcctga ccggtcaaac gtgtttattt
     6721 tgtatttttc ttccttatat ttttaaatga atgaaaagaa aatcttttat cctgcctggt
     6781 tttctctggc tgatgtctgg cttccacgcc ctggcccagt ccccggttgg atttcaatat
     6841 gtcaatttct ccagggtcga gatacacgat cccttttgga cctcccgcat ggacaaggta
     6901 gccacggtca ccctcccggt ctgtattgat cagacggaag taaaaacccc caggatcagg
     6961 aattttgaag ccgtagcggc aaaagacggg cgccgtcacc agggtatttt ttacgacgat
     7021 tcggatgtgt tcaaggcgct ggaggccatc gcttatgcca ttaaaatgga taaaaatcca
     7081 ggcctggaaa gcaaggcgga cgagtggata gaaaaaatcg ctgcggccca gttgccggat
     7141 ggatacctca atacgtatta taccctcaac ggcctggaca agaggtggac cgacatgtcc
     7201 atgcatgagg attataatct gggacatatg attgaggcgg gggtcgccta ttttgatgcc
     7261 acgggaaaaa gaaaactgct ggatgtatgc atccggatgg ttgaccattt tgcctccctc
     7321 tttggtcccg ggaaaagaca ttgggtcacc ggccaccagg aactcgaact ggccctggta
     7381 aaactatacc atacgaccag tgaccggaaa tacctggacc tggccaactg gctgctcgaa
     7441 gagaggggtc acggacatgc ctctggttat acctggaccg aatggaagga tacggcctat
     7501 gcccaggatg atgtgccttt gaggaaggtg aaagacatca aggggcatgc cgtcagggcc
     7561 atgtactatt ataccggagc ggccgatgtc gccgcaatca ctggcgatca ggaatacctg
     7621 gaagccatga aaagagtctg ggaagacgta gttttccgca acatgtatat cacggggggc
     7681 atcggcgcct cggggagcaa tgaaggattt accaaagatt atgacctccc caatgaccat
     7741 gcctactgcg aaacctgtgc cagtgtgggc atggtctttt ggaaccaaag aatgaatgcc
     7801 ctcaccggcg aggcctttta tgccgatgtt ctggaaagga gcttattcaa cggcgccctg
     7861 gacgggatct cttatgccgg ggaccagttt ttttacggca atgtcctggc ctcatcgggc
     7921 cagcataagc gcagggactg gtttggcacg gcctgttgcc cggccaatat cgcccggctg
     7981 cttgcttccc tcggaaacta tatctactcc tcttcagacc gggggatctg gattcaacaa
     8041 tatattagca gcaatacggt cattcctttg aaagaagtaa aagttccggt ccaggtcgac
     8101 accaaatatc cttatgaggg caaggtgagc atcattctga acaatgaaat ccccattgaa
     8161 acgaatcttt acctgagaat tcccggctgg gccaggggag aggcagtgcc cggaggctta
     8221 tatcgtttcc tcgacccagc tttccaatgg ccttccatca ccgtcaacgg cgtaaaaacg
     8281 aattataccc tggacaaagg ctacgccgta ttgcaccgcc agtggaaagg aggtgaccgg
     8341 atcatgctgg acattcccat gcctgtccaa cgggtagtct cggacagcat caggtataac
     8401 gaaggcaagg tggcccttca gcgcggcccc ctggtgtatt gtgtggaagg ggctgatcat
     8461 gaaggcaagg tcttcaacag catcctgccc gacaggacgg cggtaaaacc cgtttggcaa
     8521 cccaatattc tgggcggaac actcaccctg gaagccagcg tgcccgccct gcaggtcgaa
     8581 ccggattcca tttccgtgaa acaacaatgg gtcaggatga ctgccattcc ttatttcaat
     8641 tgggccaacc gggggcaaaa tgaaatgcgg gtatggttgc cgagaaggtt tgatgggtta
     8701 aaagtagatt agttagcaag ttggcaagtg aaagcgtaca tgaataacag ggtgagcctc
     8761 ctcccggcct ttaacataga gaccattcgt ggtaatattc ctccagcttt tgtctttttt
     8821 gtttttttca tcctgatcag tgaggaatcc ggcgcccagg acagctccct tttccggctt
     8881 gccggcttga tcaaaccggt catcatccat accgaccgat ggggtgtttc ccatatcgaa
     8941 gcacaaacgg aggaagacct gttttttgcc cagggatacc aggcggcccg ggaccgcttg
     9001 ttccagtttg aattgtttcg caggcaggct accgggacag tttccgaaat gctcggcccc
     9061 cgggaactgg accgggatat cggtgcgcgc ctgttccggt tcaggggcga tataaccagg
     9121 gaactgaatc attatcatcc caggggtgaa cgcatcatca aagcatttgt aaaagggatc
     9181 aataactgcg tccgggacgc gctgagccgg gaggatgatc tccccctgga gtttcgactg
     9241 ctcaatatca agccgggctt ctggactccc gaagacgtcg tatcgagaca ccagggcctc
     9301 ctgggcaatg tgcagaccga actccagacc gcgagatggc tcaggcagat cggggaagaa
     9361 cgcttgattg aactgatgaa ttaccatccc cacagcccgg acctttccct ggactccttg
     9421 cttacccggg aagccctagc cgaagacatc ctcaaatggt acaatgcttt tcgccgcccg
     9481 gtccgtttcc agaaagagga tctcctgggt atggtccgtc ccgacatcct gtctcccatg
     9541 ccgggccctg acggggaaat catgaactat aattcggaag gaagcaacaa ctgggtgatc
     9601 agtgggaata aaacacagag cgggtaccct atcatggcca atgatcccca tcgcgccatc
     9661 accattcctt cgctccggta tatcgtgcat ctgaaagcgc ccggatggaa cgtgatcggg
     9721 ggcggggaac ccatcctgcc cggggtgagc atcggccaca atgaacatgg agcctggggc
     9781 ctgaccattt ttcagacgga tatggaagat ctgtatgtgt accggatcga tcctcagaac
     9841 aggaaccggt ataagcacca tgggcaatgg ttgatgttta agaccattgc ggataccgtc
     9901 cgcgtaaaag gccattcccc ggtaaagatc gaacaccggt attccgtaca cggcccggtt
     9961 gtttttgtgg ataccctgcg gaacctggcc tatgccgtgc gttgcgcatg gttggaaacc
    10021 ggcgccgccc cctacctggc cagcctacgt atgaaccagg cccggtcctg ggccgagttc
    10081 aggcaagcct gtgcctattc ctttgtgcct gccgaaaaca tggtatgggc ggaccgcaaa
    10141 gggaatattg gctggcaggc cgtgggcctg gcgccggtga ggaaaaatca ttccggcatg
    10201 gtcccggtta tcgaccatgt gacgcacgaa tggagcggtt accagcctgt gctggaaagg
    10261 cccggtgcct tcaatcctcg ggaaggtttt attgccacgg ccaacgaaaa cctcaccccg
    10321 ccggcttatc cctatatgca gagcattggc tataattggg ctgatcccta ccgggggatg
    10381 cgtatccggg aagtattaca aggagctggc gattttctat cccggatatg gccgccttgc
    10441 aaaccgatta tttatccctg ccggccaggc tgttggtgcc cctcataacc tcgacggtaa
    10501 tgacggaccc ccagctggag aagtatgggc aatacctggc ctcctgggat tataaacttg
    10561 accccgggtc cggtgcagcg ggtttatatg tcctgacgga acgcgggatc gaagaaaaat
    10621 tattggcctt gtttttaccg gaaaacggag acggcctccg atccaacgat gtctccctca
    10681 acaatctttc cagccaggcc atggtccgat ggctatggtc tccggacggg cggttcggtg
    10741 cagacccact ggaaaaaaga aaccagatca tccgctcctc cttccttgaa gccgccgaaa
    10801 aaatgatatc ccttttcggg ccggatttcg cgcaatggaa gtatggccat gataagttta
    10861 agcacgcccg ccttcaacac gcactcagcg cggtggtgaa cgatagcctg ggaactcaat
    10921 tcaacaccca ttcccatcca cggggaggat atggcaacac cgtgaacagc accggggacg
    10981 ccgatatgca agccagcggg gcttctttcc gcatcatcgc agactgtgcg gactgggatc
    11041 aggctttggc catcaatacc gcagggcagc agggccatcc aggccagccg cattacaagg
    11101 atttgtttga gacctgggcc agggaccagt attttcctct ttatttcagc gaggccagga
    11161 taaagggggt gaccgaatcc gtgaccacac tggttccctt agaataaagg gcagcgccgg
    11221 actcccttca gatcaggcgg tatatctcca tgatggaacg gaggatcctg gggaaatcga
    11281 tgtgcaggtc tttgagtctg ccggtatgca catcgaaaat ccagccatgg accagcaacc
    11341 ctctctcctg gtatgctttt tgaaccgcgg cggttttgat cacgttgatg cattgttcct
    11401 gtacattgag ctcggccagg cggtcatatt tttcttccgg gtccgtgatg gcattgagtt
    11461 catccttatg caggcgatac acgtcccgga tattgcgcag ccagggattg aggatcccca
    11521 gatctttgga ttgcaatgat gccttcactc ctccgcagcc ataatggccg caaacgatga
    11581 tgtgccggac cttgagatgg tctacggcgt aattaattac ggacatgaca ttgagatcga
    11641 tgctgatcac catgttggca atgttccggt gaataaatac atcgccgggt tgcactccca
    11701 tcagttcttc ggcggtgacc cggctgtcgg aacagccgat ataaaggtat tcgggggact
    11761 ggccgtgggc cattttttcg aaataagcgg gatccagggc cagtttacgg gcgacccagg
    11821 cttcattgtt tttgaaaata tgttcaaaca tgaaatggtt ttaaaaggaa taccatggca
    11881 tcggctcctt attggaccgt gtctaaggcg ttggaagcag ccgtggccgg ttgcatggtt
    11941 tgaatcacca gcatgacgac caggaggatc atcaggacgc aaagcaggta gagggccttg
    12001 ccgtaattgt agaatttcag tttggcgata tggctgaggt tgtggatctg gagtaatagt
    12061 tcgtcgtagt gttcggcccg gttataagcc ctgaaccggc ggatgtattc cgaagtgggc
    12121 atggaggcga tatccccgaa aaagtacaat tttttggtag gtacatccgg cttgctggtc
    12181 acatgggccg ccaccgtgcg cacggaataa taaataaagg ggatcatcac cagcataaac
    12241 agcataaaag tgatcttata ggagagcggt tcttccgaaa gctgggaaga atcgtctatc
    12301 acgatggtca ggatgatacc cgcgatcaca aagaggaggt ttattttata gtccgcaaac
    12361 tgaatggagg actgcagggt catgtaaacg ttccagggca caaaggattc atcggcggat
    12421 cccatgatgc tttctccctg ggacaaggga taataggcat tgggtttatt ctcggtcttg
    12481 atatcctggg tttcctgcat gataggggct ataaaaatgc ttttgaaaca agaaggacga
    12541 tgagcaagcc catcatgatc gaaagcaggt aaagggcctt gctgtagttt tcaaattttt
    12601 tttttgcgat atggctgaga ttgtggacct ggagcaacaa ttcgtcataa tgctccgttc
    12661 gcgtattgga tttgaagtgt tcaataaact gactggcagg catccgcagg atatcgccga
    12721 aaaaatacag tttccgggtc tggacatccg gccggtgtat ggtatgcgca gccaccgtgc
    12781 ggatgctgta atacaagaac gggatcatga tcagcagcag gaggacaaat acaattttaa
    12841 atactaaact ttcctcgctg aaatccccga tgctttccag gacgatggac aaaataatac
    12901 cggctatgac aaagagcagg ttgattttat tgtcggcaaa ttggatggaa gactgcagat
    12961 tgccataaat cgcccagggt acaaacgatt catcatacgc agtaaggata ttggtatctt
    13021 ctgggttgat atctggtttg gacatggccg tattgcggta taaatttagt ttattgcggc
    13081 ttttcagaga aatccaattt aagaccggcc aactgaaaac aaatgaagct ttgtatggag
    13141 agtcccgctc ccggagaact gccttcagcg agggtgcctg gtctcaaaag ttaaatcttg
    13201 ttaagataat acataaatac atagaaatat gtctatattt gcatctaaaa taaagaccat
    13261 gactttaacc cagcgcatca acccggaaaa actcgaacat gcggccagca tgctccgggt
    13321 cctgtcgcac ccggtccgtt tggccatcgt cgacctgctt tttaccaacg ggcccctttc
    13381 ggtccaggaa atccaggaaa aactcggctt ggagcaggcg attgcctccc aacaccttat
    13441 cttaatgaaa gacaaagggg tgttgtcttc ggtgaaggag gggaggtgtc attatttttc
    13501 tctcctgtat cccaatatga aaaacatcat ccattgtctg gaagaatgct gcaacgctta
    13561 agtccgtgtt agcgctcatc aatcaagaac tgcctatgca tcctgaagct tatctgctgg
    13621 ctaccctgat cgggctttcc cttgggctga tcggaggagg cggttccatc ctgaccgttc
    13681 cggtcctggt ctatgtgatg ggtatttccc ccgttttgtc caccgcatat tccttgttta
    13741 tcgtcggagc cacctcgatc atcggggcca ttcaatatgc tcagcagaaa ctggttgatt
    13801 tcaagacggg gatcctgttt tgcattccca gcattgcggc cgtatacctc acccggaaat
    13861 tcctgatccc atttatccct gatccggtat tcaccctggg tcaactggtc atttccaagg
    13921 aattgctgat catgttgctc tttgcggtgc tgatgatcct ggcttccgta tccatgatat
    13981 tgccggtgcg tgaaaaacaa tatgaaaaga ttctgccggc ctggcttacc ctcctcacgg
    14041 tattgttgga gggtgccggc gtcggtgtac tcacaggttt agtaggtgcc gggggcgggt
    14101 ttttaatcat tcccgccctc gtattactca cccggatgcc tatgaaaaag gcgatcggaa
    14161 cctccctgct gatcatcgcg ctcaaatccc tgattgggtt tacgggcgac ctgggccacc
    14221 aggttttgga ctggaacctg ctcgtcctgt ttacgctgtt tgccatagcg ggcatgtttc
    14281 tcgggactgc cttgtccaag cggattggcg gggaaaaact taaaccggct ttcggttggt
    14341 ttgttctggt catgggggta ttcatcgtcg tcaacgaatt gtatttaaaa cattaataaa
    14401 tagatctaac caaataaaca cgttgtaatg aaaatcgaac aaatttatac gggatgcctt
    14461 gcacaaggtg cttattacat taccagtgaa ggggaagccg cgatcatcga tcccctgagg
    14521 gaagtcaaac cctatctgga gcgcctggaa acggacaagg tcaaactgaa gtatattttc
    14581 gagacccatt tccatgccga ttttgtttcc gggcatatag acctcagccg gcatacggga
    14641 gccccgatcg tttacggacc cgatgccgcc tgcgaattcg attgcatttc agcccgggac
    14701 ggacaggaat ttaaaatagg aaaggtgacc atcaaggtgc tgcacacccc gggccatacc
    14761 ctggaaagca ccacctacct cctgaaagac gaaaccggga aggatcattg cattttctcg
    14821 ggagataccc tctttctggg agacgtgggc cgtcccgacc ttgctcaaaa agctgcccat
    14881 atgacccagg agcaactggc cggcttgctt tacgatagcc tgaccagcaa aatcatgccc
    14941 cttgcggacg acgtcatcgt ttatcccgcc cacggtgccg gcagcgcctg cggaaaaaac
    15001 atgatgaaag agaccgtgga taccctaggc aatcagaaaa aagtgaatta tgccctgaat
    15061 caacccaacc gggaggcttt tataaaagca gttactgagg gacttcagcc tccgccggcc
    15121 tatttccctt ccaatgtggc catgaataaa aaagggtacc aggcttttga agaggtgatg
    15181 aaccacggca tgcgcgccct gagccctgcc gaatttgaag tcgtggccga agagacgggc
    15241 gccgtggtcc tggatacccg cctgcccggc gatttcgcca aaggttttgt gccccagtcc
    15301 gtcaacatcg ggttgaaagg ggattttgca ccctgggtcg gagccctgct ggccgatgtc
    15361 aaccagccca tcctgctgat cacggaagtc ggccaggaag aagaagccgt tacccggttg
    15421 agccgggttg gatttgatca tgtgctcggg cacctggccg gaggatatgc cgcctgggaa
    15481 gcctccggca aggaaaccga caccgtaaac cggatcaccg ccgaggaatt tgcctcagct
    15541 tttaaaaaag aggaatccct ggtcctcgac atccgcaggg aaggagaata tgaggcggaa
    15601 catgtggagg atgcctacag ccggccgctg gcttatatca acgactggat caaggacatc
    15661 aaccctgccc ggcattttta tatgcattgc gccagcggat accgcagtat gattgccgct
    15721 tccattttga aagcgcgcgg ataccataat ttctctgaaa tcgaaggagg atttaccgct
    15781 atcaaaaaaa cggaagtgac ctgcaccgat tatgtatgcc aatccaaaat cattaaacaa
    15841 taaccttata ataatatgtt gaaatttctc gaaagactgc ttttcggtgc cgaccgcggc
    15901 caggtaaaat cagccattga agccggcgcc ctcctggtgg atgtgcgcac gccttcagaa
    15961 tacgcatcgg gccatgtcaa aggctcgatg aatatccccc tggagagcat ccctaaaaac
    16021 ctgagtaaat ttaaaaacag ggagccgatc gtcgtttttt gcagaagcgg ggcaagaagc
    16081 gcccaggcca aggtcttgtt gaaccaaagc ggatttcagc atgtgatcaa tggcggaagc
    16141 tggtcttccg tgaataaact ggtatcaaac aattaaatat tccggtcatg aaaacccatt
    16201 atcaaatcct ggtcatcggg ggcggcaacg ccggtctttc cctcagcgcc catcttttgc
    16261 tcaaaaacaa aaagctggac atcggcattg tggaaccttc tgataagcat tattatcagc
    16321 ctgcctggac ccttgtagga ggtggggttt tcgacatcaa cgatacggta cgcaacgagg
    16381 cggattttat ccccaaacag gcggcctgga tcaaagactc ctgtgtttct ttccagcccg
    16441 aagccaatcg ggtgaccctt gcctccggcg ccgtactcac ctatgattac ctggtggtct
    16501 gccccggcat ccagctggac tggggatcgg taaaaggcct gaaggaagcc ctgggccaaa
    16561 atggggtcac caccaattac tcctttaagg tggcgcctta tacttttgaa tgccttaaaa
    16621 acttcaaagg gggaaaggta ttgttcaaca accctcatac ggccatcaaa tgcggcgggg
    16681 cgccccacaa aataatgtgg ctggccggcg attatttcag gatccacggc ttgctggaca
    16741 aggcggagat ccactattac agcggaggca cccggctctt tggggttgag aaatatgaaa
    16801 agacgcttaa aaaagtggcc gaacgatacc atgtgcatac ccactttttt gaaaaactgg
    16861 aagaggtcga cggtcccaac aaaagggcac gtttcgtggg ctttggggaa cataacaagg
    16921 gggttgaaac ctgggtggat tatgaaatct ttcacgtgac ccctccacag tccgccccgg
    16981 attttatcaa acaaagcccc ctggccaatg ccgccggctg ggtggatgtg gataaataca
    17041 gcctgcagca cgtccgttac ccaaatgtat tttccctcgg agattcctct tcactgccta
    17101 cctcgcggac cggggcggcg gtgcggaagc agaccccggt ggtagccaat aacctgctgt
    17161 ccctgatcgg gggtcaaacc ctgcaggcca agtatagtgg ctacagttcg tgccccatca
    17221 tcaccggata cggaaaactg gttctggctg aatttgatta caataaccag ccgatggaga
    17281 cttttccttt tgatcaaagc aaggagagat ggagcatgta ccaactcaaa aaacaagtac
    17341 tgcccaggat gtactggaac ctgatcctgc ggggcagggc ccagggatga taaacgttca
    17401 ctcatactaa accataaatg atcatgaatc tagttgaaac tcttagtcag ccgtggccat
    17461 ggtatatcgg cggtccgttg attggattga ttgttccggt actgttgatt gccggcaaca
    17521 aagcctttgg gatcagttcg tctcttcggc atatctgcgc cgcctgcatc ccggctaaaa
    17581 tccctttttt caagtacgac tggaaaaagg aaatgtggaa cttgttctat gcgggaggca
    17641 tcatcctggg aggtctgatt gccgctttct ggcttcccaa ccctgcaccg gttgaaattc
    17701 acccggacct gatcgcggaa ttgggcggtt atggaatcag ccagtcgaat tccttgttgc
    17761 ctgaatccat tttcaactgg aattccctgc tgagcctcaa agggtttata atgatgatcg
    17821 tgggtggatt ttttgtaggc tttggtacga gatacgccgg aggctgcacc agcggtcatt
    17881 ccatcatggg attgtccact ctgcaatggc cctccctggt tgcaacctgt tgttttatgc
    17941 tgggaggctt catcatggcc aacctgattt tacctcacct tttaaaaatg gcttaaaatg
    18001 gaaaccatca agaccaagcc cgaggctgaa cagatagaaa aaaacacgga ctttgaagta
    18061 tggtccctcg acacggctta tgtcaatgaa aaagattatc agcaaccctg gtggcatgaa
    18121 ctgaaatatg tcctggccgg tatcgttttt ggaattgtgc tcgttaagac cgaagtgatc
    18181 agttggtacc gtatccagga aatgttccgc cttcaatcct ttcatatgta cggtgtgatc
    18241 gggagcgccg tggccgtagg cgccttgtcg gttttcctga tcaaaaagtt caggatcaaa
    18301 accgtgtaca agcaggaaat tgaattccac aaaaggccct tcaccaaagg cacgattttc
    18361 ggcgggctga tttttggcct gggctgggcc ttgaccggcg cctgtccggg ccccctttat
    18421 gcgcaactgg gcagcgtggg taccgtcggc atcgtcaccc ttttgagcgc cgtggccgga
    18481 acctgggttt acggtaaaat ccaggataag ctcccgcatt aagattcaaa ccaaaccaac
    18541 cgaatgaaat attgggaaac cttcgtcaac ggttatacgg gatatgcgaa ttacctctgg
    18601 aatgaaatcc tgaatccttc ctggcacaat tatttttatt ggctgatcgg gatttccctg
    18661 tttttctggt tgctcgaaat cgccttcccc tggaggaaaa accaatcggc catccgaaag
    18721 gatttctggc tggacggctt ttacatgttc tttaattttt tccttttttc cctaatcggg
    18781 tacaacgcgg tttccaatgt cttcagccag gccttcaacg atttcatccg ctgggctttc
    18841 ggttgggaaa acctggtggc catccgggtc catcagttgc cctggctggt tcagttgggc
    18901 attttgttta tcgtccgcga ttttattcaa tggaatgtgc accggctgct gcaccggtcg
    18961 gaatggctct ggcagtttca caaggtgcat cacagcgtcg aggaaatggg cttcgccgcc
    19021 cacctccgtt accactggat ggagacgatc gtttaccgta ccctggaata catccccctg
    19081 gccatgatcg gcttcggcgt gcaggacttc ctggtcgtcc atatctttac cctggccgtc
    19141 ggtcatttta accacagcaa tatccgggtg ccgctgggtc cgttgaaata catattcaac
    19201 aatccccaga tgcacatctg gcaccatgcc gaacatattc ccaaccggta tggcgtaaac
    19261 tttggcctta ccctttcgat ctgggattat atttttaaaa cgaactatgt ccccaggtcg
    19321 ggccgtgatg aaaaactggg cttcgaccaa ctcgatgcct ttcccaaaac ttttttaaat
    19381 caatttctct atccctttta taaaaaaaat ataaaaaatg aaaaaaccaa ccatcctgct
    19441 cctcttgctg ggcattctgg cagcagcatg cagcggaatc gcacagggaa aattgaatcc
    19501 ggaccagttc aattccctgt tggctaaaga caaaaccgtc caactgatcg atgtccgcac
    19561 tccgggagaa tatgccggcg ggcatatcgc aggcagtacg aacatcgatt acaacgcccc
    19621 ggatttcctg gaccggatgc ggaaactcga caaaaacaag ccggtggctt tgtattgtgc
    19681 ggttggtggg cgcagcggta acgcagccgt taaattaaag gaactcggtt tttcgaaaat
    19741 atacgatctg gcaggaggta cccaggcctg ggccgccaac aaattacccc tggtaaaata
    19801 attctcaaac ctcgttaaac aaggagagtg gatatccccc tgatcacggt aaccttcatc
    19861 gccctggccg gcgtgttgta tgctatttcc caggtcagct ttgttttaag gaacctccac
    19921 cgtcaaccgg aacaccaggc cgcctttttg tttgcgcccc tcaacaaatt caggtttgga
    19981 cttgccttcc tttccctggg cctggccttt ttccttggcc tctgctggat gcaggggagg
    20041 caagcccatc aaaaacaatt gctccaggcc cgttcgcaga tcctcgccgc ctatgattct
    20101 tcgaaggtat ggcaggcccc ggatccctac ctgatcctga aggaagacca taaagacctc
    20161 attttgtatg gcaggcaatt gatcagcaat acttccgatt ttctgggacc ccggggcacg
    20221 gtgcgccgca tgtccaatgg aatgaactgt cagaattgcc acctggatgc ggggacccgg
    20281 ccttttggca ataattattc ggcagtattg gccacctatc ccaaattcag ggcccggtcc
    20341 gcgtccgaag aagacattac caaaaggatc aacgattgtt tcctgcgcag cctcaacggc
    20401 gattccctgg aagccggcag ccgggagatg aaggccatca ccgcttacat ccattggctg
    20461 ggccaggcgg tcccgaaaaa tcacattccc ctgggctcgg gccttgtgaa ggtggacctc
    20521 ctccagcgcc ccgcggatcc tgaaaagggg aagacggtct acatggccaa gtgtcaatcc
    20581 tgtcatggcg cggaaggcca gggattgcca aagccggaag accccttgtc caattatccc
    20641 ccgctatggg gaaaaggaag ttacaacgaa ggagccggcc tgtatcgact gtccaccttt
    20701 gcgggctatg tcaaggcaaa catgcctttt ggcgccacca aggacaatcc ccagctcagc
    20761 gatgaagaag cctgggacct ggccgccttt gtgaattccc agcccaggcc cccacacccc
    20821 ttcctggcca gggactggcc ggtgatttcc caaaaacctt acgaccatcc atttggtccc
    20881 tttgccgata cctttccggc ggaccagcat aaatacggcc cttatcaacc catcattgcc
    20941 tttcaaaaat caaagaccaa atgaaaaacc ggagaaaatt tttacaatcc ctgtccatgg
    21001 ggggccttgg cctgaccatg atcgaacccc tcaaaccggc gctgtccgct tcagggtcac
    21061 agggcgcaaa ccccggtgaa gcagggggta cccaaaaaat aaccctgcta caaacgaccg
    21121 atgtgcattg ccagatccat ccccacgatg aattgttttg ggaaaatgaa aaaatggtct
    21181 tccggaaaac ggcggggtat tcccatattt caaccctctt taaacaatac cgcaaatcga
    21241 accccaatac ctttatcgtg gataccggcg atatgttcca ggggagcatg ctgtccgtga
    21301 aaacggaggg tggcgcctat accgaaatac tcgacatcct ggcttatgat ctttatcttc
    21361 ccggcaactg ggaggtcatc taccttaaaa aacgcatgca gaccctgttg ggcgggctca
    21421 acgcccccaa ggtatgcgcc aatatgtacc acgatgcagg caatggcgct aaaggcgaat
    21481 tgatttttcc ggcctaccac acctggaccg ctgccggggt caaaatcggc tttttgggtt
    21541 ataccgatca tttggtgccc atccgtcaat cgcccatcta cagcaagggg atcatctata
    21601 ccaaacccga agaaaacctg aagtattatg tggacgtgct caaaaaccag gaacaatgcg
    21661 attacgtcat tatcctggcc cacctgggcc tgtcgcagca gatccacatc gccaacctgc
    21721 cggaatgcga gggagtggat tatattttcg gcggggacac ccatgaacgg gtgcgcaaac
    21781 ccattcaatg ccgctatgcc aaggtcgtag agccgggggc tttcggttct tttgtaggta
    21841 aactggaact gacggtgacc aatggtaaaa ttgtcggcga ccattatgaa ctggtcgaag
    21901 tcaatcccaa aaaatacaaa gccgatcccg ccatggaccg cctcatcgat aaactagaag
    21961 atccctatga ggattcgatc aaacaggtgc acggatattc caaagtgccc atgtaccggt
    22021 atttcgtggt ggagaatacg atcgacaccc tgatcgtgga tgccctgatg tggaaagcca
    22081 ataccgatat cgtcctttcc aacgggttcc gcttttgccc gccgcgtgcg gcggaccata
    22141 ccggcatggt gcccatcacg gagggatttg tttatgacat gcttccggtt gacgcggtgg
    22201 ccaggaccgc ctgggtcacg ggaaggcaac tcaaagaatg gcttgagagg gaattgcaaa
    22261 atgtatttgc cagcgaggct tccaaacgct ttgggggctg ggtggtcaag atgaaaggca
    22321 tggatattga attcaaagct tttgaagaat ttggcaaaag ggtgcagaaa atcaccattg
    22381 gcagcgagcc tgtggacccg gacaaggaat acagcgtcac cgcctgtgag cgggatgggg
    22441 atcctatgga tatgctttgc cgcataccca aggtcaggaa tccgaggagc ctggatttta
    22501 ccatgcacca ggtgatccgc gattatgtaa aaaccttttc cccggtcgat ccgacccccc
    22561 atcacaacgt caaggtcctc gatgctcccc agactttatt gtcccaggtt ttcggggtgg
    22621 attataaatt tgtttagctt tatccaaaag actcatgaat agaaaatttt tattaccggc
    22681 cctctttttt ttgggctgga ttctacccct cgcggcccag gaaccggtgg tgaacatgaa
    22741 tgaatacaag ctcgtattcc agctcaccaa cggagatacg gtggttcaca aggccacggt
    22801 caaacagatc tttaatgccc tggccgcagc gcctaaaagc aaaatagaac tggtctgcca
    22861 caacaacggg atcagtttcc tccaggtttc caaaacccaa tatatgggac agatcctgga
    22921 gctcaggaac aaaggcgtgg tcttcgccgc ctgcgaaaac acccttcgcg aacgcaagat
    22981 cgacaagacg gacatcgtcc ctcaggccgt ctttgtaccc gccggaatca ttgagat
//
LOCUS       DAOQXT010000077        22322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2683_length_22322_cov_6.951228, whole genome shotgun sequence.
ACCESSION   DAOQXT010000077 DAOQXT010000000
VERSION     DAOQXT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22322
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_22322_cov_6.951228"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3974
                     /locus_tag="PKM27_13275"
     CDS             <1..3974
                     /locus_tag="PKM27_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNR08283.1"
                     /translation="AAGGSCKITAIYTDTYLDVCGAGFKVYRKWIVTDWCTLEEEECE
                     QYIKVVDTSAPRILKPLNTIFANVSPHDCVAGVVLPALVAGTDFRDCGTVSQKYTISY
                     EDPSHPGKTVVFTGDLPSKTLYLPMGEFRVEISLVDACFNRTDTSRYVVVTDVTPPIP
                     VCNEITQVTIDPATCWAKISAKELDNGSRDNCCEVLHYAAAHMDSITYWRNYWTEKLT
                     TEVGEKSFAKERLEYEALIEDWINCYVFSDTVNFTDCGSDSIVLRVYEACGVPRYDPH
                     VFPCGPHAWFCYNTYQYIGDFNWNWFDKDGPKSCSYRPALTSLKKLDKKYEAYGTKGY
                     MQPKYEGAAQYLYCQVPFYFPSLQLMLQAKSSVGGGNAPGSYCSDRLYNDCMVLVLVD
                     DKEAPVVHELEDVTVYCDASPHYASYPRECSTGKKNGLWPGYLKDSDGTIHGYYGGYG
                     AEDEHAPDCGHYDHKKNWAPVYCREWLYLDSFETGGIIVPETYFEVPVFFDKTRPKRA
                     LAKHEFSITDNCRLDDASLSWTDEGDINSCGEGWIQRTWTMRDACGNEVTARQKVLVK
                     HRSDFEVVFPADTVMVCDYTQGTGPEVTGRPDVTDDECEQITVKFEDQVFSVDDGACY
                     KIVRTWTIFDWCIYEPNRTERYPEVIVDDRLRADTGNRECVYRNLKDNNDGYMQYIQV
                     IKVVDHEGPVIKVKDTVVCITSENCISETVRIPLSATDNCAEPEQIHFQVEIDQNATD
                     AVYTNLTYDKASIELIILGNPRELVYAASGEGRHVVHVIATDNCGNKDTVSYRLELKD
                     CKKPTPYCFSGIATVVMPSTGTVTVWAKDLDAGSFDNCTDKGGLIFSFSADKTQTSRE
                     FRCADIPNGRSQTLPVEIWVTDEAGNQDHCGTFLLLQDNAGNACPDTAGLNVMIAGKV
                     ETEGKEPVEKVRVKISGSGLDLGFETSEKGQYEFGNLPSKADYALEARRNDQPMNGIS
                     TLDLILIQKHILGTLPLDSPYKIIAADVDNDKQVTAIDLVELRKLILATYEELPNTES
                     WRIIPKTQTFEDPQNPWNFRTKIEMKQVEKDYMQEDWIGIKTGDVNASAAAHSLMGTE
                     VRENQGGLIFEVVDQQFIAGDLVSVEFRSPNFRGISGFQGTVEVDGGRWVVDGNRTPM
                     TGALYITDQNIGNRKSSEGLITMSWNSNTGVDIPDREVLFTLTFKAGKSGRLSEALRM
                     GSQVTVAESYEGKGEVSNLAFRFVTPEGNEVNTRSELYQNYPNPFDQRTVIGLRLAKE
                     GRGTLSIHDVTGRTIRSIERDWTRGYHEVWLDRREIGATGVLYYRFESGFFTDSKKMV
                     IVE"
     gene            complement(4138..6414)
                     /locus_tag="PKM27_13280"
     CDS             complement(4138..6414)
                     /locus_tag="PKM27_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR08284.1"
                     /translation="MSELTAKVDYLADLNQAQRDAVTCTDGPIMVIAGPGSGKTRVLT
                     YRISYLIEQGVPPWQILALTFTNKAAKEMRERIEKVVGSRAKYIWAGTYHSIFAKILR
                     IEAAKIGYPPNFTIYDTDDTKSLIGDIISELSLDKQVYNAGQIRARISSAKSNLISPK
                     AYLQQEELMNQDKLNRIPYFQTIYERYVNRCQKAGAMDFDDLLYQMYIMLYRNPENVK
                     EKYQSQFRYILVDEFQDTNYLQYAIVKQLIEYPGSNKNICIVGDDAQSIYAFRGATIR
                     NIFDFESDYPQHRKFKLEQNYRSTEHIVSAANQVIQNNRNQIEKTLWTDESGGNKIRI
                     IKAVSDQEEGKRVADSIVEWKNRAGLANHQFAILYRTNAQSRIVEEQLRRNNIPYKVF
                     GGLSFYQRKEIKDFIAYLRLATNPRDEEALKRVLNYPRRGIGESTIQKALEYANEHQI
                     TLWEALHRPELLGRSASAVKHFLAVIQSCMEQASTQNAYAAALYIGKASGLLDALKAD
                     RTQDGISRLDNVNALLDGIQEYLENDEAVENETGERDLSAYLQTISLLTDQDEEIKNH
                     DFVTLMSVHAAKGLEFHTVFIVGMEENLFPSYMSMADPDQLEEERRLFYVAITRAKRQ
                     LILTYATMRYQYGKPRFNDPSRFLEEIDQRHIDTAVTLRNAGPFADGPRILSTIPKLG
                     AKKENKVEVDPAQFKVSPSEEIEVGMEVLHIKFGPGKVLHIDGARDNRVATIEFHNID
                     DPQRRIMLKFSKLQIIKR"
     gene            complement(6746..8899)
                     /locus_tag="PKM27_13285"
     CDS             complement(6746..8899)
                     /locus_tag="PKM27_13285"
                     /inference="COORDINATES: protein motif:HMM:NF039722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HNR08285.1"
                     /translation="MRQTEKWKGQGWIPDVLYSVLLGLGFMQLIVGLDKINPSNTNWL
                     LIRGSDLSYHYLAWEYFKDSPWSWPPGQLVGYAYPMYNSIMYTDSIPLLAFLFKSLRG
                     ILPIDFQYFGFWYALCFVLNTRFGLALMKQAGWSRGFRWLIAPFFTGAVVLVARFGHA
                     ALCGQWVLLDVCLIYLKAKTWTIARTYSHLYAVVFLTSLIHPYLLFMVLALSVTPLLQ
                     LTWEKRIPWTKIPLYTLLASVLALTGWTLSGAFLFKGQLSEGLGKYSANLNTYFNAWD
                     VGRLGPSFPYYDQGQGEGIAYLGLGVILLILLVAGASLLRGPSNPLPSRANRSGSINF
                     FFLGSVLFFVFALSPKWAFGNHLIVDWAYNDYISRTFRGTGRFTWPLYYFILYYTFMQ
                     WHRLSWPGWVKFLCLSCMLLVQAVDLSPLWKRKPYIDSPPSSLQYVDQFKRLIASCDK
                     VLVYPPYLATIADYWDYIDFTNLAQQAGKPITTGYGARLPEHIGRAFRDSVNNLSAYL
                     ASHPGDLVITHVDSLNLHQALIQKLGGQSFQFERYRVYAPEALIKKIDLNGIDPSSME
                     RIRTQQPVRLQEYLESKASLLIAGSVYEEGLGHLGESTKQYLKEMGSHTDSLRYGASW
                     AFLIQNRKFIREAISMTDPVSLSDSFQCQGHQTLLELESGGYQAGKKISIRLGGEERS
                     SPTRGLTLVVLDSCGRFVEKVRFDTYLSDAFLIIE"
     gene            complement(8886..10619)
                     /locus_tag="PKM27_13290"
     CDS             complement(8886..10619)
                     /locus_tag="PKM27_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08286.1"
                     /translation="MSKGVLITGCLALAFASMIYYPKWKMGRTEATISWDVSGYYLYL
                     PALFIYQDLRKLEFKAAIDEKYYPSSSPYQSYAHASGNQVMKYTCGLAILYSPFFILA
                     HVVARLTGLSADGYSPIYQFMLSLGSLAYAFLGLWMLRRLLRRYFDEGLTAGSLAILV
                     FATNYFDYASITGAMSHNYLFTLYTALLLLTDQFYRDRKAQWFYLAAGISGLMVLIRP
                     TEGISLLLLMGWGIRSRAGLRERIHFFTGQLRLVIQGALFFLLAPAIQLMYWKYVAGE
                     WLVYSYQDQGFSWFEGHHILQGLFSYRAGWLVYTPVMVLCLTGILANLWLDKPWARIY
                     LFFFLASLYLTFAWNEWTYGGSLGQRALVQHYPVLLFGLASSLEVARKYPWLNRATWL
                     FIGLCMAYNLWLTHQAHRGGIYEAGMMNKAYFWQTLFKFKNDPESKKLLDTNEPLIPF
                     DETKAGLLSDTLYTPDSNYCISASASVLLYQDKAPAGKLKVMTRVFTPQKEWDVWQMA
                     QLIVQYYEGDRSVKTVFIRVNRFIGDGEEKWIDLVTKSPDPASDRLRISLWNPGSRPV
                     CLQAMKLYAAD"
     gene            complement(10620..11480)
                     /locus_tag="PKM27_13295"
     CDS             complement(10620..11480)
                     /locus_tag="PKM27_13295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR08287.1"
                     /translation="MSLRAYFRGIYYALKNQVIALDYPWDAHPRYGQAKSMNAALEQW
                     CASGAEVYRDWLKRFTAYLPFYQSIPLEGGNPASGIYWRNHYFPGLDLIGLYALVREI
                     KPGRILEIGSGNSTAVMRQAIQDEGLGTRIHSIDPHPRKDISRLADEVHTHTLESLET
                     MAVFDSLGPGDILFFDGSHLALPNSDVTVFFLEVLPRVPPGVYIQIHDIYLPFDYPPD
                     MVLRGYNEQYLLAMALLSAPASFDPVFPAWWVSRQPDFIQLTDRLIWDPLLEKGIEKH
                     GGSFWFKKRS"
     gene            complement(11480..11941)
                     /locus_tag="PKM27_13300"
     CDS             complement(11480..11941)
                     /locus_tag="PKM27_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08288.1"
                     /translation="MKYRPWLIELIWFMFTGLMVYLVILPIRSEIYGFPFEKANIWFV
                     IVFITFLRWLLLLNATPFRKNQAFKAFLGMGSIWVMLYSIRQFGLFQTFLDEKGIQSI
                     TAHLTEDRQIALAKYIPSEFIFFSVGTILVCLMMPVRMIISIWRTYNLNKS"
     gene            complement(12076..13674)
                     /locus_tag="PKM27_13305"
     CDS             complement(12076..13674)
                     /locus_tag="PKM27_13305"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNR08289.1"
                     /translation="MKRRSFLKQIPGAAAVTVIGGQTVRADHTNPLLQALYQMMYETD
                     RVLVIVQCNGGNDGLNTVFPLDQYDQLKSVRGNMLMNSSSIIKLNDKTGIHPAMAALA
                     NLYQDGKMGVIQNVGYPNFNYSHFRATDIWLSASDSDEYLDSGWAGRYLNYEYANYPV
                     GYPNAAMPDPLAIRVGEGIGLGLQMMGTNMGVSINNASDPVNLTGNIFKDPAGADYVG
                     KELSYLREVQRQTDKFGDVILAAYEKAKNLSTKYPSVNNGNTANNLTNQLKIVARLIA
                     GGLKTRIYWVSTGGFDTHSSQVDPADFTKGTHANLLQGISDNIAAFMDDIKLLGLQER
                     VAGFTFSEFGRRIRSNASLGTDHGSALPVIYFGKNIIPAVVGDNPIINPNADFNSNLP
                     MQYDFRSVYGTFLNQWFCVPQADVHSILYNQFQILPILNTANCLSTASHELNVQNGIN
                     AVSVSPNPFVHKTRVDFESFGGFVRLQVFNNHGSLVRTLLSQDLPKGKYSNDLDLEGQ
                     PTGIYYIRWENGPLQQVKGMMKVL"
     gene            complement(13702..15357)
                     /locus_tag="PKM27_13310"
     CDS             complement(13702..15357)
                     /locus_tag="PKM27_13310"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNR08290.1"
                     /translation="MAFPSPQAVTPYTGPFGKAELLHLLRRTLFGVSPADLKAFEGQS
                     LEEVVDHLLDIPILPPPPPIRAYQGRNESVFDTGVPLGTTWVNSKPIAGEQNPNGARR
                     GSYKQWWVQLMAQQERNLREKLVLFWHNHLVTDTNEAVDLAVLAYRYNVLLRQSCTGN
                     FRKLMYDITLDGAMLRYLNGEKNTAGAPDENYARELQELFCLGKGPDSQYTEDDVKAA
                     ARVLTGWNINYADNYQSVFRPNRHDKNDKSFSSFYNNKVIKGKATAGAGTEEINELLD
                     MIFAHPETARFLCRKFFTFFGYYEINGEVEQQLIQPLAELFRTSGYELKPMLRALFTS
                     EWFFKTEYRGAMIKSPADFTLGMARQMGISWPVEENAFEARYFFASQLFLQMYNQGQE
                     LGDPPNVAGWPAYYQTPSFHELWVDTATYPKRLATVDSLVVRGISTGSGTQWVHAESK
                     NKSFRADPFLFVKQLNQPHDPNVLIDQLVELFYGVSVSQAVKDSLKTTYLLKGQSTDF
                     YWSAAYVAYADNPNTTNPEARQVPRQLQDLFAYMFTAAEYHLH"
     gene            complement(15488..16765)
                     /locus_tag="PKM27_13315"
     CDS             complement(15488..16765)
                     /locus_tag="PKM27_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR08291.1"
                     /translation="MEKIGRYRWTVVGLLFFATTINYLDRQIIGLLKPVIETEFNWSE
                     KDYANIVMVFSMAYALGLFIFGRFVDTMGTKIGYLVSIVTWSLAAMAHGLVRSTGGFM
                     AVRGLLGITEGGNFPSAIKSVAEWFPKKERALATGIFNSGANIGAVVAPILVPILLVA
                     YGWRMAFVITGAIGFIWVVFWLIYYEIPTRQKRLGAAENAYIHSDPADAVAEEQPVPW
                     VRLFGIRQTWAFIAGKFIPDPIWWFFLFWLPSYFSSTFNIDLKKPSLELIFVYTFTTI
                     GSIGGGWLSGFLIHKNWPVFKARKTAMLIYALLVTPIIFIQFAQEKWTVVFMIALAMA
                     AHAAWSANIFTTVSDMFPKRAVSSVVGIGGMAGGIGSILFPLFVGFLLDKYKLLGNIQ
                     TGYNILFVICGCAYLVAWGIIHLCAPRMEKVEI"
     gene            complement(16870..17877)
                     /locus_tag="PKM27_13320"
     CDS             complement(16870..17877)
                     /locus_tag="PKM27_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNR08292.1"
                     /translation="MNRVICFGEIMLRLTPFMHQRLEQSKSFNMDFGGSESNVSAGLA
                     QLGIPVAYVSRVPDSPLGKSALSSLAQWGVDTSVSVLGGDRLGIYFVELGAGRRPSRV
                     IYDRKQSGMHSLKPGMIDWNVVFRGAAWFHWSGITPSLSASAAAATLEAINAAREAGL
                     LISCDLNYRTKLWKYGAKPSEIMPALIEATDVIVGDEDVLDAYFGLKTDGIDDAFSKL
                     SHRFPNLKYMVLTDRKGLTASHFQYHAYLYCNGRTYESQKYELPDVIDRIGSGDAFMS
                     GLICKLLPSGQNPPDLQKAIEFATACGVYKHYIPGDLNIFTESDIVSLMEGSTGGKVG
                     R"
     gene            complement(17925..18590)
                     /locus_tag="PKM27_13325"
     CDS             complement(17925..18590)
                     /locus_tag="PKM27_13325"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08293.1"
                     /translation="MSQTQIITKIITDQGILPLYFHPDEKTSLDVLKCLYDAGIRAVE
                     YTNRGEAAYRNFKQLVDLRNQSMPELKLGIGTIKNLPDAKKYVEAATDFLVSPGWVPE
                     VAEYALQNDLFYAPGCMTPSEIIAAENKGIRFIKLFPGNLLGPEFMSTIREIFPSLLF
                     MPTGGVDTTTENIEAWFAAGVSAVGMGSKLISKKLMEARDYAAITSATQNVLEIIRTI
                     RIK"
     gene            complement(18634..19659)
                     /locus_tag="PKM27_13330"
     CDS             complement(18634..19659)
                     /locus_tag="PKM27_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNR08294.1"
                     /translation="MSKKVVTLGEIMLRLSTPDFKRFVQADSLDVTYGGGEANVAAAL
                     CNYGLQGVFVTKVPDNPIGQSAINHLRRFGVDTQFIVKGGDRLGIYFLETGASMRASQ
                     VIYDRSGASIAEAGPRDFDFDSILKDADWFHTTGITPALSDSAAELAEAALKAARAKG
                     ITTSIDLNYRKKLWSKEKARSVMTRLCQWVDICIGNEEDADTTLGFKAAQTDVSKGEL
                     NLDGYKEVFRQMREKFGFKVIASSLRESHSASDNGWSALVYDGSEFYHTRQYEVRIVD
                     RVGSGDSFASGLIYGLITGMSLKDAAEFGVAASALKHTIPGDMNHATLKEVLDLMKGD
                     ASGRVQR"
     gene            complement(19823..21253)
                     /gene="uxaC"
                     /locus_tag="PKM27_13335"
     CDS             complement(19823..21253)
                     /gene="uxaC"
                     /locus_tag="PKM27_13335"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNR08295.1"
                     /translation="MPNASKTNLAPKPGFLTDDFLLHSEPARRLYHEYARDMPIIDYH
                     CHLPPDQIATNYYFKNLTEIWLKGDHYKWRAMRALGVDEKYITGGAPDREKFLQWSRV
                     VPSLMRNPLYHWTHMELKNPFGINEVLSSENAGDIYDSAGELLTNTFSAQYLLNHFRV
                     ELVGTTDDPCDTLSYHQAMKGSTRGFIVVPTFRPDPVMNIDQPESFPSYIARLGKAAG
                     MEITHFDSLLDALKNRHDWFANLGCRASDHGQVHLYAYDYTLAEVRTIFSKGLRGEAL
                     SPDEINKYRSAMQYELAVMDWEKGWVQQFHLGAIRNNNTRLLTHLGRDIGVDSIGDYL
                     QIEMMSRFFDRLDQEEKLAKTVLYNVNPTYNEAFVTMTGNFQDGSLRGKMQWGSAWWF
                     LDQKDGMTDQLNVLSNMGVLSTFIGMITDSRSFLSFPRHEYFRRILCQLFGQDIVNGE
                     LPNDLPWIGKIVQDICYYNAKSYFNV"
     gene            complement(21246..>22322)
                     /locus_tag="PKM27_13340"
     CDS             complement(21246..>22322)
                     /locus_tag="PKM27_13340"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08296.1"
                     /translation="NSYPGKLTQLLYHRFKTFKGDPSKGLSIIPTELLSDNGKILKER
                     VFRHAGFNHLETEFLQWLEEACDFCDSLVDRIVPGKLKPGNGFITLPYEDALAVQTEP
                     YCLWAIQGNASTIKRLPFAGKIEGLVVSHSIQSFREQKLRLLNGGHTALSPIAFQAGC
                     QTVGQMMDHPLIAASLEVLGEKEILPTLTGLAPDAREFFLQMKTRWRNPYIVHELKTI
                     LAQSTTKMQARNGDTFIRYFQTRGALPEMLCFGLAAFLYLFRPHRMEEGLYFAPGSHG
                     EDFEVRDQKAALLFEHWQPYYRNQEKMEDTVPLLLSDPHLFEHPFARLPGLADKITAL
                     MLAFRKTSVPDQLSQLLNSVPQHA"
BASE COUNT         5465 a         5452 c         6181 g         5224 t
ORIGIN      
        1 ccgcggcggg aggttcctgc aagattacgg cgatctatac cgatacttat ctggacgtct
       61 gcggagcggg cttcaaggtt taccgcaaat ggatcgtgac ggactggtgc accctggaag
      121 aggaagaatg cgaacaatac atcaaggtgg tggatacttc agcgcccagg atcttgaaac
      181 cgctcaacac tatatttgca aacgtcagtc cccacgattg tgtggccgga gtggtattgc
      241 ctgccctggt agcggggacg gatttcaggg attgcggaac cgtgagccag aagtatacta
      301 ttagttatga ggatccatcc cacccgggca agacagtagt gttcaccgga gatttgccgt
      361 ctaaaaccct atacctgccg atgggcgagt tcagggtgga aataagcctg gtcgatgcct
      421 gtttcaaccg gacggatacc agccggtatg tggtcgtcac ggatgtgacc ccgccgatcc
      481 cggtctgtaa cgagatcacg caggtgacga tcgacccggc gacctgctgg gcgaagatct
      541 cggccaaaga gctcgacaac ggcagccggg acaattgttg cgaggtgctg cattacgcgg
      601 cggctcacat ggacagcatt acatactgga ggaattactg gacggaaaaa ctcaccacgg
      661 aagtcggaga gaagtccttt gccaaagaac ggttggaata tgaagctttg atcgaagact
      721 ggatcaattg ttatgtattc agcgatacgg tcaattttac ggactgcggc agtgattcca
      781 tcgtgctgag ggtgtacgaa gcctgtggcg taccgagata cgatccgcat gtcttcccct
      841 gcgggccgca tgcctggttc tgctataata cctaccagta tatcggggat ttcaactgga
      901 actggtttga caaggacggt ccgaagtcat gcagctaccg cccggcgttg accagcctga
      961 aaaaactgga taaaaaatac gaagcctacg gaacgaaggg atatatgcaa cccaaatatg
     1021 aaggggctgc ccaataccta tattgccagg tgcccttcta tttccccagc ctgcaattga
     1081 tgctgcaagc caagagcagt gtgggaggag gcaatgcccc gggcagctat tgttccgacc
     1141 ggttgtacaa cgactgcatg gtgctggtgc tggtggacga caaagaggcc ccggtggtgc
     1201 acgaactgga agacgtcacg gtttactgtg atgcctcgcc ccattacgca agctacccca
     1261 gggaatgttc gacgggtaag aaaaacggcc tctggcctgg ttatctcaaa gacagcgacg
     1321 gcacgatcca cggctattat ggcggttatg gcgctgagga cgaacacgct ccggactgtg
     1381 gtcactatga ccacaagaaa aactgggccc cggtatattg cagggaatgg ctctacctgg
     1441 atagttttga aacgggaggc atcatcgttc cggaaacata ttttgaggtg ccggtattct
     1501 ttgacaaaac aaggccgaaa cgggcgctgg cgaagcatga attttcgatc acggacaatt
     1561 gccggctgga cgatgcgagc ctgagttgga cggacgaggg ggacatcaat tcctgcgggg
     1621 aaggctggat ccagcggacc tggacgatgc gcgacgcctg cggcaacgaa gtgaccgcca
     1681 gacaaaaggt attggtgaaa caccggagcg attttgaggt ggtcttcccg gcggatacgg
     1741 tgatggtctg cgattatacc cagggcacgg gcccggaggt gacgggcaga ccggacgtca
     1801 cggatgatga atgtgaacag atcacggtga aatttgagga ccaggtcttc tcagtggatg
     1861 acggggcatg ttacaagatt gtcagaacct ggacaatatt tgactggtgc atttatgagc
     1921 ccaaccggac ggaaagatat cccgaagtga tcgtggatga ccgcttaagg gcagacacag
     1981 gcaaccggga atgcgtgtac cgcaacctga aggacaataa cgacgggtat atgcagtata
     2041 tccaggtgat caaggtggtg gaccatgaag gtccggtgat aaaggtgaaa gacaccgtcg
     2101 tatgcatcac ttctgaaaac tgcatatctg aaaccgtgcg gatcccactg agcgccacgg
     2161 acaattgcgc ggagccggag cagatccatt tccaggtgga aatcgatcag aacgcgacgg
     2221 atgcggttta taccaacctg acttatgaca aggcgagcat agagctgatt atactgggca
     2281 atcccagaga gttggtatat gcagcctccg gcgagggcag gcatgtagtg catgtgatcg
     2341 cgacggacaa ctgcggcaac aaagacacgg tgagttaccg gttggagttg aaagattgca
     2401 agaagccgac gccttattgt ttttcgggga ttgccacggt agtgatgccg agtaccggaa
     2461 cagtaaccgt atgggcaaaa gacctggatg cgggcagctt tgacaattgc acggacaagg
     2521 gcggcctgat cttcagtttt tccgcagata aaacgcagac aagccgggaa ttccgttgtg
     2581 cagacatacc caacggcaga tcacagaccc taccggtgga gatctgggta acggatgaag
     2641 cgggcaacca ggatcattgc gggaccttcc tgctgttgca ggacaatgcg ggcaacgcct
     2701 gtccggatac ggcgggcctc aatgtgatga ttgcggggaa agtggaaacg gaaggcaagg
     2761 aaccagttga aaaagtgcgt gtgaagatca gtggatcggg actggacctg ggttttgaga
     2821 cgagtgagaa gggccaatac gagtttggca acctgccatc gaaggcggat tatgcccttg
     2881 aggccaggag aaacgaccag ccgatgaacg ggatcagcac cctggacctg attttaatac
     2941 aaaaacatat cctgggcacc ttgcccctgg attcacccta caagatcatc gccgcggatg
     3001 tggacaacga taagcaggtg acggcgatcg acctggtgga actgcgaaaa ctgatattgg
     3061 cgacttatga agagttaccg aataccgaaa gctggaggat catacccaaa acgcaaacct
     3121 tcgaggatcc gcaaaatccc tggaacttta ggacgaagat agagatgaaa caagtggaga
     3181 aagattatat gcaggaggac tggattggga tcaagacggg ggatgtgaac gccagcgcgg
     3241 cggcgcacag cctgatgggc acggaagtga gggagaacca gggaggcttg atatttgaag
     3301 tggtagacca gcaattcatt gctggtgacc tcgtctccgt agaattccgt tccccgaatt
     3361 tcagggggat cagcgggttc cagggtacgg tggaggtgga tggtggaagg tgggtggtgg
     3421 atggcaaccg caccccaatg accggtgcat tgtacataac cgaccagaac attggtaacc
     3481 gaaaatcatc cgaaggattg atcaccatga gttggaattc caatacgggt gtggatatcc
     3541 cggatagaga ggtattgttc accctgacct tcaaagctgg aaaaagcggg cggttgagcg
     3601 aagcgttgag gatgggatcg caagtcactg ttgcagagtc ctacgagggc aagggtgaag
     3661 tgagcaacct cgccttccgg tttgtgacgc ctgaagggaa tgaagtaaat acaaggagcg
     3721 aattatacca gaattatccc aatccttttg accagcgaac ggtgatcggc ctgcggctgg
     3781 cgaaagaggg caggggtaca ctgagcatac acgatgtgac cggcagaacg atccgctcca
     3841 tagaaagaga ctggaccagg ggctatcacg aggtctggct tgaccggagg gagatcgggg
     3901 cgacgggagt cttgtactac cggtttgaat cgggattctt cacggacagc aagaagatgg
     3961 taattgtaga ataatcgttc gcagcggcca gttttaactg gccgctatgt acaagataaa
     4021 tccttcatta cgacggccaa ggcagccgtc gcaacacagg ccgccattgc ggccagtttc
     4081 aactggcctg tgcgtaccat acgtagcggt cagttttaac tggcctaccg atacaactca
     4141 cctctttatg atctgcagtt tgctgaactt cagcatgatc cttctttgag ggtcatcgat
     4201 attatgaaat tcgatggtag ctacccggtt gtcccgcgcg ccatcaatat gaagcacttt
     4261 gccgggtccg aactttatat gcaggacttc catgcccact tcgatttcct cactgggtga
     4321 aaccttgaat tgggcaggat ccacttcaac cttgttttct tttttggcac cgagtttagg
     4381 gatggtactc agaatgcgcg gcccatcggc aaaggggccc gcattacgga gggttaccgc
     4441 ggtatcaata tgcctttggt cgatctcttc caggaaacgg ctcgggtcat taaaccgagg
     4501 tttcccgtat tggtaacgca tggtggcgta ggtcaggatc agttggcgtt tggcgcgggt
     4561 gatcgccaca taaaacaacc ttcgttcttc ctccagctga tccgggtccg ccatgctcat
     4621 ataactcggg aaaaggtttt cctccatgcc gacgataaaa acggtatgaa attcaaggcc
     4681 cttggcagcg tgcacggaca tcagcgtgac aaaatcatgg tttttgatct cttcatcctg
     4741 gtcggtgagc agggaaatgg tctgcagata ggccgataag tccctttcgc cggtttcatt
     4801 ttcaacggct tcgtcgtttt cgaggtattc ctggatgccg tcgagcaggg cattgacatt
     4861 gtccagcctg gaaatcccgt cctgggtacg atccgcctta agggcgtcga gcaggccgct
     4921 ggccttcccg atataaagcg cggcggcata ggcgttttga gtgctggcct gttccataca
     4981 agactggatc acggccagga aatgttttac ggctgaggct gagcggccca gcagttcggg
     5041 gcgatgcagc gcttcccaga gagtgatctg gtgttcattg gcgtattcca gcgccttctg
     5101 gatcgtggat tcgccgatgc ccctgcgcgg gtaattgagc acccgtttta aggcttcttc
     5161 atcccgggga ttggtggcca gcctgaggta ggcgataaaa tccttgatct ccttgcgctg
     5221 gtaaaaactc aggcctccga acactttgta gggtatatta ttgcggcgca actgctcctc
     5281 cacgatcctg gactgggcat tggtgcggta aaggatggca aactggtgat tggcaaggcc
     5341 cgccctgttt ttccattcca cgatgctgtc cgccacccgt ttgccttctt cctggtcgga
     5401 aacagccttg atgatccgga tcttatttcc tccactttca tcggtccaaa gggttttttc
     5461 gatctggttc cggttgttct ggatgacctg gttggcggcg gatacgatat gttcggtgct
     5521 ccggtaattc tgctccagct taaatttgcg atgctgggga taatcgctct caaaatcaaa
     5581 aatgttgcgg atggtggccc ctcggaaggc ataaatactc tgggcgtcat cccccacgat
     5641 gcagatgttt ttattgctgc cgggatattc aatgagttgc ttgactatgg cgtactgcag
     5701 gtaattggta tcctggaatt catctaccag gatgtatcga aactggctct ggtatttttc
     5761 tttcacattc tccgggttcc ggtagagcat gatatacatc tggtagagca ggtcgtcgaa
     5821 atccatggct ccggccttct ggcaccggtt gacgtaccgt tcgtagatcg tctggaaata
     5881 ggggatgcgg ttgagcttgt cctggttcat caactcctcc tgctgaaggt aagccttggg
     5941 tgaaatcagg tttgatttgg cggaagaaat cctggcccgg atctgaccgg cgttatacac
     6001 ctgcttgtcc agggaaagtt cagagataat atccccgatc aggcttttgg tatcgtcggt
     6061 atcgtaaatg gtgaaattag gtggataacc gattttggcc gcttcaatcc ggaggatttt
     6121 ggcaaagatg gaatggtagg tgccggccca gatgtatttg gccctcgaac cgaccacttt
     6181 ttcgatgcgc tcccgcatct ctttggcagc cttgttggta aatgtcagcg ccaggatctg
     6241 ccagggcggc actccctgtt caatcagata agaaatccgg taagtgagca ccctggtctt
     6301 gccgcttccg ggcccggcaa tcaccatgat gggcccgtcg gtacaggtca cggcatcgcg
     6361 ctgggcctgg ttgagatcgg ccaggtaatc gactttggcg gtaagttctg acattgaacc
     6421 tgcgaaatta gggctttaaa tcatgttatg gaaatgttta atgggaagat aggataaata
     6481 ggattggtag gataaatagg atttgtagga tttgtaggat tggtaggatt agtaggattg
     6541 gtaggattgg taggatttgt aggatttgta ggatttgtag gatttgtagg atttgtagga
     6601 ttggtaggat tggtaggatt agtaggatta ataggattgg taggattggt aggattggta
     6661 ggattggtag gatggttaaa gtggtagaaa ttgttaattt tttgagagac gaagagatga
     6721 agggtaaatc aggtgggtgt gatggtcatt ctatgatcaa aaaagcatcc gacagatagg
     6781 tgtcaaaacg gactttctcg acaaaccgcc cgcaggaatc caggacaacc agggtcaagc
     6841 cacgggtcgg gctggatcgt tcttctccgc ccagccggat ggatattttt ttgcctgcct
     6901 ggtaaccgcc ggattccagt tcgaggagcg tttgatgtcc ctggcattgg aaagaatcgg
     6961 aaagggaaac agggtccgtc atggatatgg cctcccggat aaatttccgg ttttggatga
     7021 ggaaggccca ggaagcgcca taccgcaggg aatcggtatg ggatcccatt tctttcaaat
     7081 attgtttcgt tgattctcca aggtggccga gaccttcttc atagactgat ccggcgatca
     7141 gcagggaagc ttttgactcg aggtattcct gcaaacggac cggttgctgg gtcctgatcc
     7201 tctccatgga tgaaggatcg attccattca ggtcgatttt tttaataagc gcttccgggg
     7261 catagaccct gtacctttcg aattgaaaag attgccctcc caatttttgg attaaggcct
     7321 ggtgcaggtt caaagaatcc acgtgggtga tcaccaggtc cccgggatgg gaggcgaggt
     7381 aagcggacaa gttattgaca ctgtcccgga acgcccggcc gatatgctcc ggaagccggg
     7441 ccccgtaacc ggtggtgatg ggttttccgg cttgttgtgc aaggttggta aaatcgatat
     7501 agtcccagta gtcggcgatc gtggctaaat atggagggta aacgagcacc ttgtcgcagg
     7561 aagcaatgag tcgtttaaac tgatccacat attgaagcga agaaggcggg gagtcgatat
     7621 atggtttccg cttccagagc gggctaagat ccacggcctg taccaggagc atacacgaca
     7681 agcaaaggaa tttcacccag ccgggccagg acaaccgatg ccactgcatg aaagtgtaat
     7741 aaagtataaa ataatacaag ggccaggtga atcgcccggt gccgcggaaa gtccggctga
     7801 tgtaatcgtt ataagcccag tccacgatca aatggtttcc aaatgcccat ttcgggctga
     7861 gtgcaaaaac aaaaaacaac accgatccca ggaaaaaaaa attgatgctg ccggacctgt
     7921 tagcacggga aggcaaaggg tttgagggtc ccctcaaaag ggaagcgccg gcaaccagca
     7981 ggatcagcaa gatcacgccc aggcccaggt aagcaatgcc ctccccctgg ccctgatcat
     8041 aatacggaaa ggagggaccc aggcgtccca catcccaggc attaaaatag gtattcaggt
     8101 tggcggaata tttacccagg ccttccgaaa gttgcccttt gaacaaaaat gcgccgctca
     8161 gggtccatcc ggtgagggcc aggacggagg ccagcagggt atacagcggt attttggtcc
     8221 aggggatccg tttctcccac gttaattgta atagaggggt taccgacagg gccagtacca
     8281 tgaataacag ataagggtgg atcaggctgg ttaaaaacac cacagcatat aaatggctgt
     8341 aggtcctggc tatcgtccaa gtcttggctt tgagatagat caggcataca tctaacaata
     8401 cccattggcc gcaaagggcc gcgtgcccaa aacgagccac caggacgacc gcgccggtga
     8461 aaaaaggcgc gatcaaccaa cgaaaacccc tcgaccagcc cgcctgtttc atcaaagcca
     8521 ggccaaaccg ggtattgagc acaaaacaga gggcgtacca gaatccgaag tactggaaat
     8581 caatgggtaa aattccacgg agggatttaa acaggaaagc cagcaagggg atggaatcgg
     8641 tgtacatgat gctgttgtac atcgggtagg catatcccac caattgaccg ggaggccagg
     8701 accaggggct gtctttgaaa tattcccagg ccagataatg ataagagagg tcactgcccc
     8761 ggatcagcag ccagttggta ttggacggat tgatcttatc caggcctacg atcaactgca
     8821 taaaacccag gccaagcagg acggaataca gcacatccgg gatccagccc tgccctttcc
     8881 atttttcagt ctgccgcata taatttcatt gcttggagac agaccggcct tgaccccggg
     8941 ttccataaac ttatcctgag ccggtcggag gcagggtccg gacttttggt cacgaggtct
     9001 atccattttt cttcgccatc gccgatgaag cggtttaccc ggataaacac ggtctttacg
     9061 ctccggtccc cctcgtagta ctggacgatt aactgggcca tctgccagac gtcccattct
     9121 ttttgagggg tgaagacccg ggtcatcacc tttaattttc cggccggagc tttgtcctgg
     9181 taaagcaata ccgaagcgga agcggagata caataatttg aatccggagt ataaagggta
     9241 tccgataaaa gtccggcctt cgtctcatca aatggaatca ggggttcatt cgtatccagc
     9301 agttttttgg attcaggatc gtttttaaat ttaaataggg tctgccagaa ataggcttta
     9361 ttcatcatac ccgcctcata aatccctccc cggtgagctt ggtgggtgag ccagagattg
     9421 tatgccatac aaagtccgat gaacagccag gtcgccctgt tcagccaggg atatttcctg
     9481 gccacttcga ggctggatgc caggccgaat aagagtaccg gataatgctg caccagggca
     9541 cgctggccca gactgccccc ataggtccat tcgttccagg caaaagtcag gtaaaggctg
     9601 gcgagaaaaa aaaagaggta gatccgggcc cacggtttgt ccagccaaag attggccagt
     9661 atccctgtaa ggcaaaggac catgaccggg gtatagacca gccagcctgc acgatagctg
     9721 aaaaggccct gcaggatatg gtgcccttca aaccagctga agccctggtc ctggtaactg
     9781 taaaccaacc attccccggc aacatatttc cagtacatta attgtatcgc aggggcaagc
     9841 aggaagaaca gggcaccctg gattaccaac ctaagctgac cggtgaagaa gtgtatccgt
     9901 tcccttaagc cagcccggga ccggatcccc caacccatca ataaaagaag actgatccct
     9961 tcggtcgggc ggatcaatac catcaggccc gatattcctg cggcgagata aaaccattgt
    10021 gctttccggt cccggtagaa ttggtccgtg agcaggagga gggcagtgta aagggtaaat
    10081 aaataattgt ggctcatcgc cccggtgatg gaggcatagt caaaataatt ggtggcaaag
    10141 accaggatgg ccaggctccc tgcggtcagc ccttcgtcga aataccttct caggagtctg
    10201 cgcagcatcc ataagcccag gaaggcatag gccaggctgc ccagcgaaag cataaactgg
    10261 taaatgggtg aatatccatc ggcagaaagg ccggtgagcc tggccacgac atgggccagg
    10321 atgaaaaaag ggctgtacag gatggccagc ccgcaggtgt atttcatcac ctggttgccg
    10381 gacgcatggg cataagactg gtaggggctg ctgcttggat aatatttttc atcaatggcc
    10441 gctttaaatt caagctttcg aagatcctgg taaataaaca aggcgggtaa atagagataa
    10501 taaccggaga catcccagga gatggtggct tccgtacggc ccattttcca tttaggataa
    10561 taaatcatgg aggcaaaggc cagcgccagg catccggtga tcaacactcc ctttgacatc
    10621 tagctccttt ttttaaacca gaatgagcct ccgtgttttt cgattccttt ttccagcaaa
    10681 ggatcccaga tcaggcggtc cgtcaactga atgaaatcgg gctggcggct gacccaccag
    10741 gccgggaata ccggatcgaa ggaagccggt gcggacagca gggccatagc caataaatat
    10801 tgttcgttgt aaccgcggag caccatatcc ggtgggtaat caaaaggcag gtaaatatcg
    10861 tgaatctgga tataaacgcc gggagggacc ctgggcaaga cctcgaggaa aaagaccgtc
    10921 acgtccgaat tgggaagggc aaggtgcgag ccgtcaaaaa ataggatgtc tccggggccc
    10981 agggaatcaa aaacagccat tgtttccaag gattccaggg tatgggtgtg gacctcgtcg
    11041 gcaagccggg agatatcctt tcttggatgg ggatcgatgg aatgaatccg ggtacccagg
    11101 ccctcatcct gaatagcctg gcgcatcaca gcggtgctgt tgccggaacc gatttcgaga
    11161 atcctgcccg gctttatttc cctgaccagg gcatagaggc cgatcaggtc caaacccggg
    11221 aaataatggt ttctccaata aatgccggaa gcggggttgc cgccttccag tggaatggat
    11281 tgatagaatg ggaggtaggc ggtaaagcgt ttcaaccaat cccggtaaac ctccgcgccg
    11341 gaagcacacc actgttccag cgcagcattc atggattttg cctggccata ccggggatgg
    11401 gcatcccatg gataatccag ggcgatcacc tggtttttga gcgcgtaata gataccccgg
    11461 aaataagccc tcagggacat caggacttgt tgaggttata cgttctccag atggaaatga
    11521 tcatccggac gggcatcatc aggcagacta aaatggtacc caccgaaaaa aaaatgaatt
    11581 cggaagggat gtatttcgcc agggcgattt gccggtcctc cgtgaggtgg gccgtgatcg
    11641 actggatgcc tttttcatcc aggaaggtct ggaacaagcc aaactgccgg atggaataga
    11701 gcatgaccca gatggacccc atgcccagga aggctttgaa ggcctggttt ttccggaaag
    11761 gagtggcgtt caacaagagc agccagcgaa gaaaggtaat gaagacgatc acaaaccaga
    11821 tattcgcttt ttcaaagggg aaaccgtaga tctccgaccg gataggcagg atcaccaggt
    11881 aaaccattaa accggtaaac ataaaccaga tcagttcaat cagccatggc ctgtatttca
    11941 tgaagggatg aagatattaa aattgaatgg ctgaccgggt agttttaact ggatgggaaa
    12001 cccgtcactt ccagtctctt taagagaatc cctgaagttt ctccatttgg ctgtcccaac
    12061 gaataaattc tttggctata gcaccttcat catccccttc acttgctgga gaggtccgtt
    12121 ttcccagcgg atgtaataga tgccggtagg ttggccttcc agatccaggt cattgctgta
    12181 tttccctttg ggcagatcct ggctgagcag ggtccggacc aggctgccat ggttgttgaa
    12241 cacctggagg cggacaaaac cgccgaaact ttcaaaatcg acccgggtct tatggacaaa
    12301 tgggttgggg gagacgctga ccgcatttat tccgttttgc acattcagtt catggctcgc
    12361 cgtcgacagg cagttggcgg tattcaggat gggcaggatt tgaaactggt tgtacagtat
    12421 ggaatggaca tccgcctggg gtacgcaaaa ccattggttc aggaaggtac cgtagacgga
    12481 acggaaatca tactgcatgg gcaggttgct gttgaaatcg gcgtttggat tgatgatggg
    12541 attgtccccc accacggccg gaatgatgtt ttttccaaaa taaatgaccg gcagggccga
    12601 accgtggtcg gtacccaggg atgcgttgga ccggatgcgc ctcccgaatt cggaaaaggt
    12661 gaagccggcc acccgttcct gcagccccag caatttaata tcatccatga aggcggcaat
    12721 attatccgaa atcccctgga gcaaattggc atgggtacct ttggtaaaat cggccgggtc
    12781 tacctggctg gaatgggtgt caaaacctcc cgtactcacc cagtatatcc tggtcttcag
    12841 gcctcccgca atcaagcggg ccacgatttt aagctggttg gtcaggttgt ttgcggtgtt
    12901 gccgttgttt acgcttggat atttggtgct caggtttttt gccttttcat acgccgccag
    12961 gatcacatcc ccgaatttgt cggtttgtct ttgcacttcc cgcagatagg acaattcctt
    13021 gccgacgtag tccgccccgg caggatcctt aaaaatattc ccggtgagat ttaccgggtc
    13081 gctggcattg ttgatggata cccccatatt ggtgcccatc atctgaaggc ccagtcctat
    13141 gccttctccg acccggatgg ccagggggtc aggcatggcc gcattgggat agccgaccgg
    13201 ataattggcg tattcgtagt tgaggtaacg gccggcccaa ccgctgtcga ggtattcgtc
    13261 ggaatctgaa gcgctcagcc agatgtcggt ggcgcggaaa tgggaataat taaaattggg
    13321 gtaacctaca ttctggatga cccccatctt gccatcctgg tacaggttgg ccagggcagc
    13381 catggccggg tgaatgccgg ttttatcatt tagcttgatg atgctggagg agttcatcag
    13441 catattgccg cggacggatt ttaattgatc atactgatcc agggggaaga cggtattcag
    13501 tccgtcattg cctccgttgc actggacaat gaccagcacc cggtcggttt catacatcat
    13561 ctggtacaaa gcctgcaaaa gggggttggt atggtccgca cggaccgttt ggcctccaat
    13621 caccgtcacc gcggcggcac ctggaatctg ttttaaaaag gaccgtcttt tcatgatctc
    13681 ttggattatt gggaaaatgg attaatgcaa atgatattct gcggcggtaa acatgtacgc
    13741 aaaaaggtcc tggagttggc gggggacctg tctggcctcc gggttggtgg tattgggatt
    13801 gtcggcataa gcgacataag cggctgacca gtaaaaatcg gtggactgtc ctttcaagag
    13861 gtaggtcgtt ttaagactgt ccttcacggc ctgggacacg gaaactccgt aaaacaattc
    13921 caccagctgg tcgatgagca cattcggatc atgcggctga ttgagttgtt ttacaaataa
    13981 aaaagggtcc gcccggaagg acttgttttt actttccgcg tgtacccact gggtgccgct
    14041 gccggtgctg atgcctctca ccaccaggct gtcgaccgta gccagccgct tgggataggt
    14101 ggctgtgtcc acccaaagtt catggaaaga cggggtctga taatatgccg gccatcctgc
    14161 gacattgggc gggtcgccca actcctgtcc ctggttgtac atctgcagga ataactggga
    14221 tgcaaaaaaa taccgggctt caaatgcatt ttcctccacg ggccaggaga tgcccatctg
    14281 cctggccatg cccagggtaa aatcagccgg gcttttgatc atggccccgc ggtattccgt
    14341 tttaaaaaac cattcactcg tgaacaaggc cctgagcatc ggtttaagtt catatccgct
    14401 ggtccggaac agctcggcaa ggggctggat caactgttgt tccacctccc cgttgatttc
    14461 ataatagcca aaaaaagtaa agaatttcct gcagaggaaa cgcgccgtct ccggatgggc
    14521 aaagatcatg tccagcagtt catttatttc ttctgttccg gcgccggcag tggctttgcc
    14581 tttgatcact ttattattgt aaaaggagga aaaggacttg tcgtttttat catgccggtt
    14641 gggacgaaag acggattggt agttgtctgc atagttgatg ttccatcccg taaggacacg
    14701 cgccgcggcc tttacgtcgt cttcggtgta ctgggaatcg ggtcctttcc caaggcaaaa
    14761 caattcctgc aactcccggg cataattttc atcgggagcc cctgcggtat ttttttcgcc
    14821 gttgagataa cgcagcatgg ctccgtccag ggtaatgtca tacatcagtt tcctgaagtt
    14881 gccggtgcag gattgacgca acaaaacatt atagcgatag gccaggacgg ccaggtccac
    14941 cgcttcattg gtatcggtga ccaggtggtt gtgccagaac aagaccagtt tttccctgag
    15001 gttcctttcc tgctgcgcca tgagttggac ccaccattgt ttataggacc cccttcttgc
    15061 gccgttggga ttttgttctc cggcgatggg ttttgaattg acccaggtgg tgcccagggg
    15121 aactccggta tcaaaaacac tttcattcct tccctgatag gcccggatgg gagggggagg
    15181 cgggaggatg gggatgtcca gtaaatggtc caccacttct tcaaggcttt gcccttcgaa
    15241 tgctttcaaa tcagcgggac ttacgccaaa cagcgtccgc ctcaggaggt gcaacaattc
    15301 cgccttgccg aatggtccgg tataaggggt gaccgcttgt ggactgggga aagccataat
    15361 ggaagttttc tgctattaat atacgtaaga caagccttgg acgatagtca cggcgggtcg
    15421 tttaattgta tttaaaaatt taacgcgaaa acctgcccat catttcaccc agtacaaaag
    15481 gccgaggtca aatctcgact ttttccatcc ggggtgcgca gagatggata atgccccagg
    15541 cgaccaggta ggcgcagccg caaatgacaa agaggatgtt gtaaccggtc tgtatattgc
    15601 ccaggagttt gtatttgtcc agtaaaaaac cgacaaagag aggaaacagg atgcttccga
    15661 ttccgccggc catgcccccg atgcccacca cgctgctcac tgcccgtttg gggaacatat
    15721 cagaaaccgt ggtaaaaata ttggcgctcc atgccgcatg ggccgccatc gccagggcga
    15781 tcataaacac cacggtccac ttttcctggg caaactggat aaaaataatc ggggtgacca
    15841 ggagggcata gatcagcatg gcggttttgc gggctttgaa gaccggccag tttttatgga
    15901 tcaggaaacc tgaaagccat ccgccgccga tgctgccgat ggtggtgaag gtgtacacga
    15961 aaatgagctc aaggctgggt tttttaagat caatgttgaa ggtggaagaa aaataagagg
    16021 gcagccaaaa caggaaaaac caccagatgg ggtccggaat aaacttgccg gcgatgaagg
    16081 cccaggtctg ccggatgcca aacaagcgaa cccatggaac aggctgttct tcggctaccg
    16141 catcggccgg atcgctgtgg atataggcat tttcagccgc acccagcctt ttctgcctgg
    16201 tagggatctc ataataaatc agccagaaaa ccacccagat aaagccgatg gcgccggtga
    16261 tcacaaaggc catcctccag ccataggcta ctagaaggat cgggaccagg ataggggcga
    16321 ccaccgcccc gatgttggcg cctgaattaa agatgccggt ggccaaggcc ctttcttttt
    16381 tgggaaacca ttccgccacc gatttaatgg ctgatgggaa attgccccct tcggtaatgc
    16441 ccagcaaacc gcgcaccgcc ataaagcctc cggtgctgcg gaccaggcca tgcgccatgg
    16501 cggccaggct ccaggtgacg atggaaacca ggtatccgat tttggtgccc atggtgtcca
    16561 caaagcggcc aaaaataaaa agcccaaggg cataggccat ggaaaagacc atgacgatat
    16621 tggcataatc tttctccgac cagttgaatt cggtttctat gaccggcttc aacagcccga
    16681 taatttgcct gtccaggtaa ttgatggtcg ttgcgaaaaa caacaagccc accacggtcc
    16741 aacgatatct tccgattttt tccatagtag tcagtagtca atagtcagta gtcaatagtc
    16801 aatagtcagt agtcaatagt cagtggtcag tggtcagtag tcaggaatca gtagtcagtt
    16861 gggtttgaac tatcttccaa cttttcctcc cgtgcttcct tccatcaggg atacaatatc
    16921 cgattcggta aatatattca aatctcccgg tatatagtgt ttgtataccc cgcaggccgt
    16981 ggcaaattca atggcctttt gcaaatccgg cgggttttgc ccggatggga gcagcttgca
    17041 gatcaggccg ctcataaagg catcgccgct tccaatgcgg tcaatgacat ccggcagttc
    17101 atatttctgg gattcgtagg tccggccatt acaatataaa tatgcatggt attgaaaatg
    17161 actggcggta aggcctttgc ggtctgtcag gaccatgtat tttaaattag gaaaacgatg
    17221 actcagtttt gaaaaagcat catcaatgcc atcggtcttc aggccaaaat aagcgtccag
    17281 tacgtcttca tcgccgacga tcacatcggt ggcttcaatc agggccggca tgatctccga
    17341 tggtttggcc ccgtacttcc aaagttttgt acggtagttg agatcgcagg aaatcaacaa
    17401 gccggcttcg cgggcagcgt taatggcttc cagggtggcc gccgccgctg aagcactcag
    17461 ggagggggta atccccgacc agtggaacca agccgctccg cgaaagacca cattccaatc
    17521 gatcattccg ggtttcaggc tgtgcatgcc actctgcttc cggtcataga tcacccttga
    17581 aggtctccgg cccgcaccca gttccacaaa ataaatgccc agccggtccc ctccgagtac
    17641 acttaccgaa gtgtccacac cccattgggc caggctggaa agggcgcttt tacccaatgg
    17701 cgaatccgga acccggctta cgtaagccac cgggatgccc aattgggcca gtcccgccga
    17761 aacattggat tcgctccccc cgaagtccat attgaaggac ttcgattgtt ccaggcgctg
    17821 atgcatgaaa ggagtcaggc ggagcatgat ttccccgaag cagatcaccc ggttcataat
    17881 tcccgtgttt gaggagcaat cgtcatcagg ctggtcactc cccgttattt gatcctgatg
    17941 gttctgatga tttccagcac attctgcgtg gcggaagtga tcgcggcgta atccctggcc
    18001 tccatcaatt ttttactgat cagtttgctg cccatgccca ccgcgctgac ccctgccgcg
    18061 aaccaggcct caatattctc tgtggtagta tccacgccgc cggtgggcat aaacaggagg
    18121 gacggaaaaa tctccctgat ggtactcatg aattccgggc cgaggaggtt gcctggaaat
    18181 aacttgatga atcggattcc tttgttttcg gcggcaatga tctcggaagg agtcatgcag
    18241 ccgggggcat aaaagaggtc gttctgcagg gcgtattctg caacctcggg cacccatccg
    18301 ggactgacca aaaaatcggt ggcggcttcc acatattttt tggcatcggg aaggttctta
    18361 atggtgccaa tacccagttt caattcgggc atcgactgat tgcgaaggtc gaccagttgt
    18421 ttaaaattcc ggtaagcggc ctctccccgg ttggtatact ccaccgcgcg gatccctgca
    18481 tcatacaggc acttcagcac atccaggctg gttttttcgt ccgggtgaaa atacaggggc
    18541 aggatgccct ggtccgtgat gatttttgtg atgatctggg tctggctcat tttaaatggt
    18601 ttaaatagta acagatggat tccttagccg gatttaccgt tgcacgcgtc cgctggcgtc
    18661 tcctttcatc aggtcaagca cttctttcag ggtggcatgg ttcatgtctc ccggaatggt
    18721 atgtttgagg gcgctggcgg ctacgccgaa ttcggccgca tctttaaggc tcatgccggt
    18781 gatcagtccg taaatcagac cgctggcaaa ggagtccccg ctgcccaccc ggtcaacgat
    18841 tcgtacttca tactgccggg tatgataaaa ttccgaacca tcatatacca gggcgctcca
    18901 accgttgtcg ctggcgctat gactctcccg cagggaggaa gcaatcacct tgaatccaaa
    18961 tttttcacgc atttgcctga acacttcttt gtacccatcc aggttcagtt ctcccttgct
    19021 gacatcggtt tgggccgctt tgaaacccag cgtggtgtcg gcatcttctt cattgccgat
    19081 acaaatgtcc acccattggc aaagccgggt catcacggac ctcgcttttt ccttgctcca
    19141 gagttttttg cggtaattga ggtcaatgct ggtggtgatg ccttttgccc gcgccgcttt
    19201 aagagccgct tctgcaagct cggcggcgct gtcactcagt gcgggggtga tgccggtcgt
    19261 atgaaaccag tccgcatctt tcaggatgga gtcaaagtca aaatcccggg gacctgcttc
    19321 cgcgatcgaa gccccggaac ggtcatagat cacctgggag gcgcgcatgc tcgccccggt
    19381 ttccaggaaa tagatcccca gccggtcacc gccttttaca atgaattggg tatccactcc
    19441 aaagcggcgc aggtgattga tggcggattg cccgatcggg ttgtccggga ctttggtgac
    19501 gaatacgccc tgcaaaccat agttgcataa agcggcggct acgttggctt ctccgccccc
    19561 ataggtgaca tccaggctgt cggcttgtac aaaacgctta aaatccgggg tggacaatcg
    19621 aagcatgatc tccccaaggg ttactacttt ttttgacata aaggcaaagt gtttaaatta
    19681 ggttatacct gaacggaagg gcatgggata ccacccatca gaaaagccat ggtggacttt
    19741 tcgcttaaaa cggataaccg gccctgaccg tccgcggcaa aagtaagagg ccggggccta
    19801 tccacaaagg ctttaccggg gtttatacgt tgaaatagga ttttgcatta taataacaga
    19861 tatcctggac gatcttgcct atccagggca aatcattggg taattcccca ttgacaatgt
    19921 cctggccgaa aagctggcac aggatgcgcc tgaaatattc atgccttgga aaggagagga
    19981 aggaacggct atccgtgatc atgccaatga aagtggacag gacccccata ttggacagta
    20041 cattgagctg gtcggtcatc ccgtctttct ggtcaagaaa ccaccaggcg ctgccccatt
    20101 gcattttacc ccttaaactt ccgtcctgga aattgccggt catggtgaca aaggcctcgt
    20161 tataggtcgg attgacatta tacagcacgg ttttcgccag tttttcttcc tggtccaggc
    20221 ggtcaaagaa tctggacatc atttcgatct ggagataatc tccgatgctg tcgaccccga
    20281 tgtcgcggcc aagatgggta agcagccggg tattgttgtt acggatcgca ccgaggtgaa
    20341 actgctggac ccatcctttc tcccagtcca tcaccgccag ttcatattgc atcgccgagc
    20401 ggtatttatt gatttcatcc gggctcaagg cttctccccg aaggcctttg gaaaaaatgg
    20461 tccggacttc agccagggta taatcatagg catataaatg gacctggccg tggtcggagg
    20521 cccggcaacc caggttggca aaccagtcgt gccggttttt cagcgcatcg agcagggaat
    20581 caaaatgggt gatttccatg ccggcagcct tacccaacct ggcgatatag gaagggaagg
    20641 actccggttg gtcgatattc atgaccgggt cgggccggaa ggttggaacc acgataaacc
    20701 ccctggtgct gcctttcatg gcctggtggt acgaaagggt gtcgcaggga tcatccgtgg
    20761 tgcctaccag ttccaccctg aaatgattca gcaggtactg ggcggagaag gtattcgtca
    20821 gcaattcgcc tgccgagtcg tagatgtccc ctgcattttc ggaagagagc acttcattga
    20881 tgccgaatgg gttcttcagt tccatatggg tccaatgata taggggattg cgcatcaggc
    20941 tgggcaccac cctggaccat tgcaggaatt tttcacggtc cggtgctccc ccggtgatgt
    21001 atttttcatc caccccgagg gcgcgcatgg ctctccattt gtaatggtcg cccttcagcc
    21061 agatttcggt aaggttttta aaataataat tcgtagctat ctgatcggga gggaggtggc
    21121 aatgatagtc gatgatgggc atatcccgcg catattcatg gtaaagtctg cgcgcgggtt
    21181 ctgaatgaag gagaaagtcg tcggtcaaaa atccgggttt gggggcaagg ttggtcttgg
    21241 aagcattagg catgttgtgg tacggaattt aataattgtg ataattgatc aggcacggag
    21301 gtctttcgga aggcaagcat cagggcggtt attttatctg cgagtcccgg caagcgggca
    21361 aagggatgtt cgaagagatg gggatccgac aacaaaagag gtacagtatc ttccattttt
    21421 tcttgattcc ggtaataagg ctgccagtgc tcaaacaata aggcagcctt ctgatccctg
    21481 acctcaaaat cctctccatg actccccggt gcaaaataaa ggccctcttc catccggtgg
    21541 gggcggaaaa ggtataaaaa ggccgccaat ccaaaacaaa gcatttcggg caaggcaccc
    21601 ctggtctgaa aatagcggat aaaggtgtcc ccgttccggg cctgcatttt ggtcgtggac
    21661 tgggccagga tggtctttag ttcgtgtacg atatagggat tacgccatcg ggttttcatt
    21721 tgtaaaaaga attcccgggc atccggggca agcccggtca gggtgggtag gatttccttt
    21781 tcgcctaata cctccaggct ggcggcgatc aggggatggt ccatcatttg tcctaccgtt
    21841 tggcaccctg cctggaatgc gattggggac aaagcggtat gccctccgtt gagcagcctg
    21901 agtttttgtt cccggaagga ctgaatggaa tggctcacaa ccaggccctc tatttttccc
    21961 gcgaaaggca atctttttat cgtggaggca ttcccctgga tggcccaaag gcaatagggt
    22021 tcggtctgga ccgccagggc atcctcataa ggcaaggtga taaaaccgtt tccgggcttt
    22081 aatttaccgg gcacgatccg gtcgaccagg ctgtcgcaaa aatcgcaggc ctcctccagc
    22141 cactgtaaaa attccgtttc caggtggtta aacccggcgt gccggaatac ccgttccttg
    22201 agtatttttc cgttgtcgct gagcaattct gtggggatga tgctcaggcc tttggaaggg
    22261 tctcctttga aagttttgaa acggtgatac aaaagctgcg taagtttacc gggatacgaa
    22321 tt
//
LOCUS       DAOQXT010000078        22238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2694_length_22238_cov_6.292702, whole genome shotgun sequence.
ACCESSION   DAOQXT010000078 DAOQXT010000000
VERSION     DAOQXT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22238
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2694_length_22238_cov_6.292702"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKM27_13345"
     CDS             <1..505
                     /locus_tag="PKM27_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR08297.1"
                     /translation="LRAVQTRDLFSNHVDQSRLKVRSRSIPLRDSMEGKLFESLLMHR
                     IYYNDQVKELDEKTLIYFAYGTNGQLDNRVLEAYAQQLVSRLKNTRDDILLVGHTDDD
                     ATEESNLKLGLQRAELIKYLLVNKGLPAGRIKTSSKGESSPIAPNNTEANRSLNRRVE
                     LFLVPKQ"
     gene            516..1124
                     /locus_tag="PKM27_13350"
     CDS             516..1124
                     /locus_tag="PKM27_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08298.1"
                     /translation="MDSLQISLCDLPWWAVLASWLLPALLGYFWGILQYGRYKNKAKK
                     AEKELSSASKRIVNLENEVNECRKAYEKVSREKDKLTQPPAPAQVHAPGQDHTGSPAS
                     VENITTTEIIHATRNIFGYSIRRNDLKIVDGIDPKIEKLLHEIGIYTWADLESVDLHY
                     LSNVLREAGYPQVNVESWAYESALARQEKWDELKAFQEKEKS"
     gene            complement(1135..1839)
                     /locus_tag="PKM27_13355"
     CDS             complement(1135..1839)
                     /locus_tag="PKM27_13355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNR08299.1"
                     /translation="MIFPIGDDQVQGGRKPYFAYLFIGLNLAVFIYQALMPPPVLEQF
                     VLKFGSIPSEILQGKHLYTLLTSMFLHGGWVHLIGNMLFLWVFADNIEAVIGNFNFIL
                     FYLAGGLAATMAHVLAGPGSTVPAVGASGAISAVLGAYLVLFPASRIRVLVVYFFRSF
                     YMPAIYFLGFWIIQQLISGFFSLGTITDRAQVSGVAWWAHIGGFAFGLVAGWVARQML
                     EKPQQLGRGFSGDDLV"
     gene            complement(1893..2777)
                     /locus_tag="PKM27_13360"
     CDS             complement(1893..2777)
                     /locus_tag="PKM27_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNR08300.1"
                     /translation="MKSSFADCVEAIYRSRARVVCCGIGKSALVAQKVVATLNSTGTS
                     ALFLHAADAVHGDIGMLSRGDFLCCFSKSGETEELRLVIPIAKNIGCPVVAITAKPDS
                     YLAMQADYKIITPVDKEADPNNLAPTASTAAQMAAGDALAVSLLHLRGFTAEDFAKFH
                     PAGSLGKQLYLHVYDIYPLHGKPVNAPSDTLGQVILTISKNRLGATVVTDESGRPAGI
                     ITDGDLRRMLEKSPDYRTVLAAEIMNPNPKCIEDQALAMSALETMRSHNISQLVVLRD
                     EIYVGMIHLHDLVREGLI"
     gene            3017..5293
                     /gene="recQ"
                     /locus_tag="PKM27_13365"
     CDS             3017..5293
                     /gene="recQ"
                     /locus_tag="PKM27_13365"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNR08301.1"
                     /translation="MSETLAQKKKNKTTRTTAATKPSKSKDKASYGLTEALQQYFGFD
                     QFKDQQEEIIQSVLDGKDTFVIMPTGGGKSLCYQLPALMMEGTAIVVSPLIALMKNQV
                     DSIRGYSQGDEVAHFLNSSLTRTQIRQVKESITNKHTKLLYVAPETLTKDENLEFLRG
                     VQVSLIAVDEAHCISEWGHDFRPEYRRIREMIDALGHSVPVMALTATATPKVQSDIVK
                     TLDMKNHRVFKSSFNRTNLYYEIRPKVSKSQTIKEIIQILRTQPGGSAIVYVQSRKST
                     EELAQVLEVNGIKAAPYHAGLDTKLRAKIQDDFLMQEIDVICATIAFGMGIDKPDVRF
                     VIHYDIPKSIENYYQETGRAGRDGLEGRCVAFYSAKDILTLEKFLRDKDAAEREMGAH
                     LLDEVVAFAETAACRRVFLLNYFGEQFASEDCHQMCDNCRHPKEKVEVKQDMKLVLQS
                     IQQTKENYLVNLIVDFITGKATKEIKDYGFDEYDLFGKGHDHEPLYWHSICRHGILNG
                     LILKDIEQYGTLKLTEAGRKFIGKPTSIKIPLNTNFEAQAELDEEEAEARVVALDQTL
                     YKMLDDLRKSLSRTLKVPPYVIFQDGSLQDMATEYPVTLEDLAKVNGVNLNKAQRYGK
                     KFVELIAKYVEENEIDRPQEIQIKQIADKSKTKVAIIQSIDRKMPLEDIARTCQMKMD
                     ELMDEMDIIVTSGTKLNLDYYLEDNVDETIQEEIYDYFMSAESDDAELAYKSLKEEDI
                     TIEEIKLVRLKFLSEMAM"
     gene            5509..5961
                     /locus_tag="PKM27_13370"
     CDS             5509..5961
                     /locus_tag="PKM27_13370"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNR08302.1"
                     /translation="MLIWIINGPNLNLLGRREPEIYGHRSFEQYFEELKAAFPRLDLR
                     YFQSNHEGAILDHLQSIGFGQSDGLVFNPAAFTHTSIAIGDCLAAIQLPCVEVHISDI
                     YKREAFRHHSYIKPYCIDSIVGQGLDGYRSAVAKLSEYLSAREARTQS"
     gene            complement(6219..6725)
                     /locus_tag="PKM27_13375"
     CDS             complement(6219..6725)
                     /locus_tag="PKM27_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08303.1"
                     /translation="MRDRKFLLLFFLLLPGIHVPGQAFLILESSGRVKPRKYPVGSQI
                     RVHFKGEAGSAWDTYTIKGFDLPAQCIIVSDTYCLPIRDLDGFDVSPGSRGALARTMR
                     KFFIQWSFFSLAQYVFKPPLYPFHFAVAGGTALVWLYSSLFLKGEKKLNARHRLRLID
                     LSLPQPRA"
     gene            6872..7342
                     /locus_tag="PKM27_13380"
     CDS             6872..7342
                     /locus_tag="PKM27_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08304.1"
                     /translation="MTSLLLPHRFKKPGWFILIPAALAGILISIMDFDAQLLKGTVFA
                     IANDPLLGEDHFFSFIKTDLTNTVVGSLFIIGGLLVGFSREKKEDEFIANLRLTSLLW
                     AVWVSYILLLLAFLFIYGAVFFSVMVYNMFTVLIIFIIRFNFLLYKNAVPANEK"
     gene            7332..7532
                     /locus_tag="PKM27_13385"
     CDS             7332..7532
                     /locus_tag="PKM27_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR08305.1"
                     /translation="MKNNIRVERAIKGYTQEELANRVSVSRQTINAIEANKYIPSTAL
                     AIKIAREFGKRVEDIFYLDDTD"
     gene            complement(7638..8120)
                     /locus_tag="PKM27_13390"
     CDS             complement(7638..8120)
                     /locus_tag="PKM27_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08306.1"
                     /translation="MNHRFLHPGPSGDLILLPIEQEMDDNQSFFSFPEGKETLEMSVE
                     YYHRVGYQPPWIGYFVQHGERIVGSAGYKGPPREGRIEIAYGVFEPFRQKGLGGAICR
                     ALTRLALMTDPSLTVTARTLPEINFSSRILEKNGFRNSGIVIDPDDGPVWEWVYERPV
                     "
     gene            complement(8117..8863)
                     /locus_tag="PKM27_13395"
     CDS             complement(8117..8863)
                     /locus_tag="PKM27_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNR08307.1"
                     /translation="MKDLGSLITAIELHDVEGIRSSFARGISPNDLYQGIPLFRELTS
                     EYTRSPRFSLCVKAFVDFGLKGEDPALVAVLLDDAPALEKLLAADGALIHKTYSMRCA
                     YTPMHEVSLLHLCAEFNHVACADLLFRYGADINSAAGFDEFGFGGQTPVFHTVNQNSN
                     QSADMLTFLLDRAAKLQVTVPGLIWGKGYEWETLIPAVNPISYAMMGLLPQMHRSETV
                     IAETVSKLLRHAYGIKYEPANVPNAYLARP"
     gene            complement(8938..9375)
                     /locus_tag="PKM27_13400"
     CDS             complement(8938..9375)
                     /locus_tag="PKM27_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNR08308.1"
                     /translation="MPFSKILIAVDSSDYAVNAAKKGFELAQETNAAVALVFVVDAAK
                     AIGNVDAGILPMEAEEMLRKEAQQTIDQLAEQSHDPSRLSKLTPEGNPQDEILQTAED
                     WGADLIVMGTHGRTGLVHFLVGSIAESVVRHSKVPVMVVPTKE"
     gene            9630..11360
                     /locus_tag="PKM27_13405"
     CDS             9630..11360
                     /locus_tag="PKM27_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNR08309.1"
                     /translation="MKYIFACLLCLNLFGGYGQTLPTLFETSQARETPDYWEIIRWWK
                     TLDQHSDKVHLRTMGMTDAGQPLHLAVVSQAGDTDFERMRKENKRVILVNNGIHPGEP
                     DGIDASMLLARDIAFGKIRMPDQLVLAIIPVYNIGGCLDRSPDYRVDQNGPEAFGFRG
                     NSQNLDLNRDFIKCDSKEARAFAEIFHLADPDVFLDNHVSNGADYQHIMTLICSQHNK
                     LGGIMGEFMNKDFEPGIYASMKKKGYDLIPYVNAFGDRVDQGWPEFMDGPRYSSGYAA
                     LWHSFSFVAETHMLKAYDQRVQATYELMLSFMDFTSGHSKQIKELREATKNQVKSQRE
                     FTITWNPDRTRSSERLYKGYESGYRKSEVSGLDRLYYDRTRPYEKTIPFYNHYLPGQK
                     VQKASAYVIPQGWWKVIDLLRLNKVNMMPLDRDTLMEVEVYRIEEYESSSRPFEGHHI
                     NTKVRTSISVGKMSFRKGDYLVPLDQVANRFLVEVLEPAAPDSYFTWNFFDAVLGQKE
                     GYSAYAFEDIAAEYLQRHPDLKEKLAERRQSDSTFARSARAQLYYVYEHSPWIEPAFM
                     RYPVYRVNSQ"
     gene            11590..12786
                     /locus_tag="PKM27_13410"
     CDS             11590..12786
                     /locus_tag="PKM27_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08310.1"
                     /translation="MKYLLIFLSLFSQIQAFAQKRFDIRIQSESRLREAIVSVPTTPP
                     PPGGYPIVFMCHGTSGEASNFYNPVGWKELGQAENFITVFPSALVWCYLDQREDNKET
                     HLSKFVCGDLLSQVCEKDMNALISDILFFKKIIQLISDTLKVDQNKIFMTGFSNGSSM
                     AHKISMEATDVFSASGGSSGALHALDSTTPRSRRIPMWYMVGTRDDRYFSDLFPVELP
                     FGGDSILIYLQGSIRRALVCQGLTEEFTKTVTPVSHIYTWNTCRTGEACAPYVFMLNK
                     DQTHEYPNGQNHPVDAPKILWNFFNKPPAVKMTTPIRETKSSGVELVFYPNPATDFLS
                     IKFKGLKPGPGYLKIVNGSGKVMVRQNILAEGADRVDLSGWPSGIYYAMFTSRTGSLV
                     KLFTKV"
     gene            complement(12814..13392)
                     /locus_tag="PKM27_13415"
     CDS             complement(12814..13392)
                     /locus_tag="PKM27_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNR08311.1"
                     /translation="MRRIIAAINMTLDGNFDHTSVNPDEEVHHHYEELLNSGDAVLYG
                     RITYQLMEFWPGLIKNPSGNKALDEFALSIDRITKIVFSNTLQPEDIWWRNSRLAKQG
                     LREEIMELKSTLPSSNGNGNKDILIGSPSLIAAALNLNLIDEFQLCVHPVIAGKGMSL
                     FKSIDGRINLKLLKTKSFGSGAVIFYYGPENE"
     gene            complement(13389..14099)
                     /locus_tag="PKM27_13420"
     CDS             complement(13389..14099)
                     /locus_tag="PKM27_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR08312.1"
                     /translation="MFTESAEFYDLLYRFKDYQKEAGDLIQRIKRIRPGAKTVLDIGC
                     GTAEHHKYLQDEFQIDGLDLHEGFIEIAKRKNPSGHYYIENMINFKLGKKYEVILCLF
                     SSIAYVKTYKKLKSALTCFYQHLEEDGLVFVEPWLTPDHWQDGKIHMLTYEGEHLKIC
                     RMNRSASKGRLSLLNFHYLVGTPEKGVRHIKEKHELALFTQDEMNKAFHESGFEVSYD
                     EEGITGRGLYFGRKKAAK"
     gene            complement(14219..14575)
                     /locus_tag="PKM27_13425"
     CDS             complement(14219..14575)
                     /locus_tag="PKM27_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR08313.1"
                     /translation="MLTAIHPKLPMRDKAATREFYIHRLGFNEFGSMDHPAYLMVEKD
                     QVQIHFFGFPGLDPKENYGQVYIRTDNIDELYRSLLDRHISIHPNGPLENKPWGQREF
                     SVLDPDHNLLTFGQKI"
     gene            complement(14634..15569)
                     /locus_tag="PKM27_13430"
     CDS             complement(14634..15569)
                     /locus_tag="PKM27_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08314.1"
                     /translation="MGNAIFLWITLTLLTGWGLVKQGGHQSHPAPPKVIIPVLCLADD
                     KMTSGWKSALASRMSKPALDSVAALALPLSQEQKEWIHLIESKKAGWGLILDSLAIPF
                     RNCIAPDTTILFMGNSGNDDGFTFGLNTVCLDLRALYNNYGPAAIPENSGRIDRIFAH
                     EFTHLLHKQWAELNHFELRTFKDSILWECLYEGIGMYRSLSSKWLPADGVLPGITIKT
                     LASLYPVFTDRLLAAASSPSPDEASKNSIVSNLSRGPVDKKWGAFTVAIWLALESKGD
                     ERKLAFWIDHHLASPISLAKKYLPATDKKRLEQWY"
     gene            complement(15634..16356)
                     /locus_tag="PKM27_13435"
     CDS             complement(15634..16356)
                     /locus_tag="PKM27_13435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR08315.1"
                     /translation="MSKEKILAAYEQMAEAYNALVMHKPHNAYYDRPNTLRLLPNVKG
                     KTILDAACGPGVYAEILMDLGALVTGFDLSPKMVDLAIQRNPGRGTFFVHDLSKPLER
                     IGDHSCDIVLCALALHYIEDWSMTIKEFHRVLKSGGVLVISIEHPFYEFNFFKSKKYF
                     EIEPVKCTWRGFGSPVEVHSYRRPLSACLSPLTGNGFYIDELVEPKPSKEFEPLDPKH
                     FRELNEFPAFMCIRAVKRKGPT"
     gene            complement(16361..16843)
                     /locus_tag="PKM27_13440"
     CDS             complement(16361..16843)
                     /locus_tag="PKM27_13440"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR08316.1"
                     /translation="MTKSKILQMHNVGVVVESLDKAISFFTEIGLKLEGRALVEGEWA
                     GRVTGLGSQTVEIAMMVTPDGHSRLELSRFLDPPTVSDHRTAPVNALGYLRIMFTVDR
                     LDELVSRLTRHGAQLVGEVVRFEDAYRLCYLRGHEGLLIGLAEQLGHKTATDILENAG
                     "
     gene            complement(16938..17333)
                     /locus_tag="PKM27_13445"
     CDS             complement(16938..17333)
                     /locus_tag="PKM27_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08317.1"
                     /translation="MKKYAVLKLIGLAMLTMITLVLISFLEVAVYSYLINPGQDAAVY
                     DAHALSSAPYISGIFGFIIFFLVVRYWKKKKYPNLTTLVFLFPLIYVLLDLIIVTAAG
                     VRWAEFLIIFLIANGAKFLGSFLGYKLKP"
     gene            complement(17372..18349)
                     /locus_tag="PKM27_13450"
     CDS             complement(17372..18349)
                     /locus_tag="PKM27_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNR08318.1"
                     /translation="MAPDFDTTLVSQYIRSIFQDSRGHLWFGTIGEGVVRYDGKTLTY
                     FSSPDGFHQNTVYAINEDKNGNLWFGTDQGVYKYGAAGAPGKSLPTGVAAQAGFRNYN
                     QKDGLSQLDITRKGILVDQSGRIWIGTHGGVFKYNPSADTVGGQSFSLFNPLPPINVT
                     GILEDQRGNIWIASSNRGVFRYDGVNLSNIAEKEGLGDNYAGGLAEDKAGNMWFTMKN
                     GICKFDGHTLTEYTPRDGLCGTEFWGLIIEASGIIWVTARGCTTRFDPSLPLPHPKAF
                     TVFTSAEGLTCCVQSMYQDRAGNMWWGAGQGLFRFDGSRFYQVKKNGPW"
     gene            18348..18599
                     /locus_tag="PKM27_13455"
     CDS             18348..18599
                     /locus_tag="PKM27_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08319.1"
                     /translation="MAAAAKFLITLGLDMDGMGTCEEFIISCPLQEVRMDKKKQNINL
                     FMECYKLDGAKSFRPGCQRYNELVSNNPLWTRLHGGFLR"
     gene            18702..20057
                     /locus_tag="PKM27_13460"
     CDS             18702..20057
                     /locus_tag="PKM27_13460"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HNR08320.1"
                     /translation="MLKRDLHFILNGLFILSAGFGFILCTCTDPLNHESVPKGEAYLS
                     FTGNGAWCWFSDPRAIYFEGKHRRTFAGWVDDSGNITVGFYDHDLKRIETTVLHKNFE
                     KDDHDHPSLLMDRQGKLMVFYSRHASPDPIYLLKAKHAEDLSEWEPPLRLDLNDSITY
                     PGGSNTYTYTNLCRLADEKDRLYLFWRGADFKPNLSTSDDGGKVWSPGKIFILPDRIY
                     KDRRPYLKVASDNKAVIHFAFTDGHPNVEPANSIYYVKYSGNAFYKAGGEKIADWSAL
                     PIQPKSADLVYDATKTNEKAWIWDVAENEEGKPVIVFSTFPNDTSHVYYYSIWDNNQW
                     MNHRLVDSGPWFPQTPEGEKEREPYYSGGIVLDHEDPSNLYLSRLKNKKFEIEKWSTA
                     NKGVDWKVEEITHNSENHNVRPVVIRNYSEPDSLKVLWMNVEKYLHYTDYRASIKMNI
                     R"
     gene            20288..21742
                     /gene="asnS"
                     /locus_tag="PKM27_13465"
     CDS             20288..21742
                     /gene="asnS"
                     /locus_tag="PKM27_13465"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNR08321.1"
                     /translation="MYNQRTKIKALLSSEATHFQTTVMGWVRSFRNNQFIALNDGSTN
                     NNLQVVVELGMLDESNLKRITIGACIRASGELLPSMGKGQKVELKASAIEILGDSDAE
                     TFPIQPKRHSLEFLRENAHLRFRTNTFGAVFRVRHALAFAIHQFFNSKGFVYMHTPVI
                     TGSDAEGAGEMFRVTTLPIDGSAPRNEDGSINFKSDFFGRSTNLTVSGQLEGELAAMA
                     FSEIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDLEDNANLAEEFIKYIIRYATEY
                     CSEDLAFLAQRLEEEEKALPQEKRSGMGLLEKLDFVVKNHFERITYTQAIDILRESNH
                     NKKKKFQYPIDQWGIDLQSEHERYLVEKHFQKPVILTNYPAKIKAFYMRLDENCPPDR
                     QTVAAMDILAPGIGEIVGGSQREERMDHLLRRMREMHIPEKELWWYLDTRRFGSCVHS
                     GFGLGFERLVQFVTGMNNIRDVIAFPRYPGNAEF"
BASE COUNT         5805 a         5524 c         5504 g         5405 t
ORIGIN      
        1 gctgcgtgcc gttcagacca gggacctttt ttcaaatcat gtggaccaga gccgcctgaa
       61 agtaagaagc aggagtattc ccctgcgtga ttccatggaa gggaaattat ttgaatccct
      121 cctgatgcac cggatttatt acaatgacca ggtcaaagaa ctggatgaaa agacactgat
      181 ttattttgca tacggtacca atggacaact ggacaaccgg gtccttgagg cctatgccca
      241 gcagttggtc agccggctga aaaacacccg ggatgacatc ctgctggtcg ggcataccga
      301 tgatgatgcc accgaggagt ccaacctcaa gctgggcctg caaagagcag aactcatcaa
      361 atacctgctg gtcaacaagg gcctgcccgc gggcaggatc aaaacttctt ccaaagggga
      421 gtccagcccg atcgcgccaa acaacaccga agctaaccgc agcctcaacc gcagggttga
      481 gctcttcctc gtcccaaaac aataatcaaa tgaatatgga ttccctgcaa atctcattgt
      541 gtgacctgcc ctggtgggca gtcctggcct cctggctctt gccggccttg cttggttatt
      601 tctggggtat cctgcaatat ggcagatata aaaataaagc caagaaggcg gaaaaagaac
      661 tttcttccgc gagcaaacgg atcgtaaacc tggaaaatga ggtcaatgaa tgccgtaagg
      721 cctatgagaa agtgagccgg gaaaaagaca aattaacaca gccccctgcc ccggctcagg
      781 tacatgcccc tggccaggac cacaccggat cgcctgcctc agtggaaaat atcaccacga
      841 cggagatcat tcatgccacc cgaaacatct ttggatattc catccggcga aacgacctga
      901 aaattgtgga tggcatcgac cctaaaattg aaaaattatt gcacgagatc gggatataca
      961 cctgggccga cctggaaagc gtggacctcc actacctgtc caatgtgctc cgggaagcgg
     1021 gttatccgca ggtgaatgtg gaatcctggg cctacgaaag cgccctggcc cgacaagaaa
     1081 aatgggacga gctcaaagcg tttcaggaaa aagaaaaatc ctgatgtaca agttttagac
     1141 cagatcatcg cctgaaaagc ccctgcccag ctgctggggt ttctcaagca tttgccgggc
     1201 cacccagccc gccaccaaac caaaggcaaa tcctccgata tgcgcccacc aggccacgcc
     1261 gcttacctgg gcccggtcgg tgatggtccc cagggaaaag aatccgctga tcaactgctg
     1321 gatgatccag aatcccagga aataaatagc cggcatataa aaggaccgga aaaaataaac
     1381 caccagtacc cggatacgcg atgccggaaa aaggaccagg taagctccca gaactgcgga
     1441 aatcgccccg ctggccccca ccgcgggaac cgtgcttccg ggaccggcca ggacatgggc
     1501 catggtggcg gccaggccgc cggccaggta aaagaggata aaattaaagt tgccgatgac
     1561 cgcttcaata ttgtctgcaa agacccataa aaaaagcatg ttcccgatca ggtgtaccca
     1621 gccgccgtga aggaacatag aggtcagcaa ggtatacagg tgtttgccct gcagaatctc
     1681 ggaggggatg gatccgaact tcagcacaaa ttgttccaga accggcggag gcatcagggc
     1741 ctggtaaatg aacacggcca ggttgagccc gataaagaga taggcaaaat agggctttct
     1801 gccgccttgc acctgatcgt caccgatggg gaatatcata tgaatattaa aaacaaaaaa
     1861 ggaacaagat agtttctttg ctttcgagga gcttagatca ggccttcgcg taccaggtcg
     1921 tgcaggtgga tcatgcctac gtatatttca tcccgcagca ccaccagttg gctgatgttg
     1981 tggctgcgca tggtttcgag ggcggacatg gccagggcct ggtcctcgat gcatttcgga
     2041 ttcggattca tgatttcggc cgcaaggacc gtccggtaat ccggactttt ttccagcatg
     2101 cgacggaggt ctccgtccgt aatgatccct gccggccggc cggattcatc agtgactacc
     2161 gtggctccca acctgttttt ggatatggtc aggatgacct gacccagcgt gtccgaagga
     2221 gcgtttaccg gctttccgtg caggggatag atgtcgtata catgcaggta cagttgttta
     2281 cccagggagc cggcaggatg gaatttggca aagtcctctg ccgtgaatcc gcgcaggtga
     2341 agcaggctca cggccagggc gtcaccggcg gccatctggg cggccgtgct ggcggtaggg
     2401 gccaggttgt tgggatcggc ctccttgtcc accggtgtta tgattttgta atcggcttgc
     2461 atggccaggt aggaatccgg cttggccgtg atggccacca ccgggcatcc aatgtttttt
     2521 gcaataggaa tgacgagcct taactcttcg gtctccccgc tttttgaaaa acaacagagg
     2581 aagtcccccc tggagagcat gccgatatct ccgtgaacag catcggcggc gtgcaggaac
     2641 agggcggaag tcccggtgga attcagggtg gccaccactt tttgggcgac cagggcgctt
     2701 ttgccaatcc cgcagcaaac gacccgggcc ctggaccggt agatcgcttc cacgcagtcc
     2761 gcgaaagaac ttttcaaatg atttgctaat gcggaaaggg cctctatttc gattccgatg
     2821 catcgcctgg ctatatccaa aatctccttt tccaaatcgc gaaaaaaatg taatttcggc
     2881 tcctttttaa aaagacctaa aagtaataaa acatcgcaag ccgccattat tagatgacca
     2941 aacggaataa aatttaaaaa agggctcgct ttttttaatg ggaaagatga aatatcttca
     3001 gtaaattaca gggacgatga gtgaaacatt agcgcagaag aaaaaaaaca aaaccaccag
     3061 aaccaccgcc gctacaaaac cctctaaaag caaggataaa gcctcgtatg ggctgacgga
     3121 agccctccag caatatttcg gatttgacca gtttaaagac cagcaggaag aaatcataca
     3181 gagcgtgctg gatggcaagg atacttttgt gatcatgccc accggaggag gaaaatccct
     3241 ctgttaccag ctgcccgccc tgatgatgga aggcacggct attgtggtct ctcccctgat
     3301 cgcactgatg aaaaaccagg tcgattcgat ccgcggttat agccagggtg atgaggttgc
     3361 ccatttcctt aactcatcat taacacggac ccagatcagg caggtcaagg agtccatcac
     3421 caataaacac accaaactcc tttatgtagc tcctgagacc ctgaccaagg atgaaaacct
     3481 ggagttcctg cggggggttc aggtttccct gatcgccgtg gacgaagccc actgtatttc
     3541 cgaatggggc catgatttcc ggcccgagta ccggaggatc cgggagatga tcgatgccct
     3601 gggccactcc gtgccagtga tggccctgac cgcgaccgcc accccaaagg tgcagtccga
     3661 catcgtaaaa accctggata tgaagaatca ccgggttttt aaatcttcct tcaaccgcac
     3721 caacctgtac tatgaaatcc ggcccaaagt cagcaaatca cagaccatca aggaaatcat
     3781 ccagatcctg aggacccagc cgggaggttc cgccattgtc tatgtgcagt cccggaaatc
     3841 tacggaagaa ctcgcccagg tgctcgaagt caacggcatt aaagccgctc cttatcatgc
     3901 cggcctggac accaaattgc gggccaagat ccaggacgat ttcctcatgc aggagatcga
     3961 tgtgatctgc gcgacgatcg cttttggtat gggcatcgat aaacccgatg tacgttttgt
     4021 gatccactac gacatcccca aaagcattga aaattattac caggaaaccg gccgggccgg
     4081 ccgggacggc ctcgaaggaa ggtgcgtcgc tttttattcc gcaaaagaca tccttaccct
     4141 ggaaaaattt ctgcgggata aggacgcggc ggaacgggaa atgggggccc acctcctgga
     4201 tgaggtggtc gcttttgccg aaaccgcggc ctgcagaagg gttttcctgc tgaattattt
     4261 tggcgaacag tttgcctcgg aagattgcca ccagatgtgt gacaattgcc gtcatccgaa
     4321 agagaaggtg gaggtcaaac aggacatgaa actggtgctt caatccattc agcaaaccaa
     4381 ggaaaattac ctggtaaacc tgatcgtcga ttttattacc ggcaaggcga ccaaggaaat
     4441 caaggattac ggatttgatg aatatgacct ctttgggaaa ggacatgatc atgaacccct
     4501 gtactggcat tccatctgca ggcatggaat cctgaacgga ctcatcctga aagacatcga
     4561 acaatacggc acgctcaaac tgaccgaggc gggccggaaa ttcataggca aacccacttc
     4621 gatcaaaatt cccctgaata ccaattttga agcccaggcc gagctggacg aagaggaagc
     4681 ggaagcccgg gtggtcgccc tggaccaaac cctgtataaa atgctggacg atctgcgcaa
     4741 gtccctttcc aggacgctca aagtcccgcc ttatgtgatt ttccaggatg gttccctcca
     4801 ggacatggcc accgaatatc cggtcaccct ggaggacctg gccaaggtca atggggtcaa
     4861 cctcaacaag gcgcagcggt acggtaaaaa attcgtagag ctgatcgcga aatatgtgga
     4921 agaaaatgag atcgaccggc cgcaggaaat ccagatcaaa caaatcgcag acaaatccaa
     4981 aaccaaggtg gccatcatcc agtccatcga ccggaaaatg cccctggaag acatcgcccg
     5041 cacctgccag atgaaaatgg atgagctcat ggacgagatg gacatcatcg tcacctccgg
     5101 caccaagctc aacctggatt attacctgga agacaatgtg gatgaaacca tccaggagga
     5161 gatctacgat tattttatgt ccgctgaaag cgatgatgcc gaattggcct acaaatccct
     5221 gaaagaagaa gacatcacga tcgaggaaat aaaattggta agactgaaat ttttgtcgga
     5281 aatggcgatg taggagtcaa tagtcaatag tcaatagtca atagtcaata gtcaattagt
     5341 taatagtcag ttgttaacag ttttaaatca gacgggctat ttcaacggac cattggccgt
     5401 ggatcactga ttcctgacct gatacctgac tactgactac tgattcctga ctactgacta
     5461 ctgatacctg actactgact actgatacct gactactgac tactgcttat gctgatctgg
     5521 atcatcaacg gccccaatct gaaccttctg ggaaggaggg aaccagaaat ttatggccac
     5581 cgcagttttg aacaatattt tgaagaatta aaagccgcct ttccccggct ggacctgcgg
     5641 tatttccagt ccaatcatga aggcgccatc ctcgatcatc tgcagtccat tggttttggc
     5701 cagtcggatg gcttggtttt taacccggcc gctttcaccc atacttccat tgccatcggc
     5761 gactgcctgg ccgccatcca actaccctgc gtggaagtac atatctcgga catatacaag
     5821 cgggaggcct tccgccacca ttcctacata aaaccttatt gtatagacag cattgtgggc
     5881 cagggcctgg atgggtaccg gtcggccgtg gcaaaactgt cggagtattt gtccgcaagg
     5941 gaagcgcgga cgcaatctta gattgtcctc aaacttgaat aagttgacgt gaaaccgcat
     6001 gtcccggcct atcatctttg tattgtcaat caagtttatc cctttatact aaaagattga
     6061 tggtgaaagg acggaaaatc cagagtagca gctggatgag tccaagacgg caatttctta
     6121 ctgaaaatca agtccaatag gtggtaggaa aagaagttgc cgtccaccct ttttttaagc
     6181 atttctgaat cgtcctgctg attactccaa attttttttc aagccctggg ttggggcaaa
     6241 ctgaggtcaa tgaggcgaag ccggtgacgg gcattcaatt ttttttctcc tttcaaaaaa
     6301 aggcttgagt aaagccagac cagagccgtg cccccggcta cggcaaaatg aaaagggtag
     6361 aggggcggct tgaatacata ttgagccagg ctgaaaaaac tccattgaat aaaaaatttc
     6421 cgcatggtcc gggccagggc gccccggctc cctgggctga catcaaaacc gtccaggtcc
     6481 cggataggaa gacaataggt gtccgaaacg atgatgcatt gagcgggcag gtcaaagcct
     6541 tttatggtat aagtatccca ggccgatccg gcttcaccct tgaaatgaac gcgaatctgg
     6601 ctgcccaccg gatatttcct cggtttgacc cttccactgc tttcgaggat caggaatgcc
     6661 tggccgggca catggattcc cggcaaaaga agaaaaaaaa gaagaaggaa tttcctgtcc
     6721 cgcatatcag gctaaagaaa cgaattttcc aaaggaagct gctaaatccg gggttaatac
     6781 actcagttgt gtaaagtatt atagataaaa tgtaaaataa acttgacaaa ttgtaaacca
     6841 gcatttactt ttgtctttat aaacattatt tatgacctct ttattattgc cccatcgatt
     6901 caaaaaacca ggctggttca ttttgatccc ggccgccctg gcaggcatcc tcatcagcat
     6961 tatggatttc gatgcccaat tactgaaagg aaccgtgttt gccattgcca atgatccttt
     7021 attaggcgag gaccattttt tttcttttat caaaaccgac ctcaccaata ccgtggtcgg
     7081 atcgctgttt atcatcgggg gcctcctggt tggtttttcg agggaaaaga aggaagatga
     7141 atttatagcc aatctcaggc tgacctcctt gttgtgggcc gtgtgggtca gttatatcct
     7201 cctgttgctg gcattcttat ttatctacgg cgcggtcttt ttttccgtga tggtatacaa
     7261 tatgtttacg gtgctgatca tatttatcat ccgtttcaat ttcctccttt acaaaaacgc
     7321 ggtaccggcg aatgaaaaat aatatccggg tcgagcgtgc cataaaaggt tatacccagg
     7381 aagaactggc caaccgggtc tccgtcagca ggcagaccat caatgcgatc gaagccaata
     7441 aatatattcc ttccaccgca ctggccatta aaattgccag ggaatttggc aagagggtgg
     7501 aggacatttt ttatctggat gataccgact gagtaccggt tcttcttttc cgttttggat
     7561 caaagagcag gtccggaccc ggatccaagg ttcggtcaga acaaaaagat tcgtccgtgt
     7621 gccgtgaaag cggcagctca gacgggccgt tcataaaccc attcccaaac cggcccgtca
     7681 tccgggtcga tcacgatacc ggaattccgg aatccgtttt tttcaaggat gcggctggaa
     7741 aaattaattt ccgggagggt cctggccgtc acggtcaggc tggggtccgt catcagggca
     7801 agccgggtta aagcacggca aatagcgcct cccaggcctt tttgcctgaa gggctcaaag
     7861 acgccatagg cgatctcgat cctgccttcc cggggtgggc ctttgtatcc ggcggatcct
     7921 acgatccttt ccccatgttg aacaaaatac ccgatccagg gaggctggta gcccacccgg
     7981 tggtaatatt ccacggacat ttccagggtt tcctttcctt ccgggaatga aaaaaaggat
     8041 tggttatcat ccatttcctg ttcaatgggt aagaggatca ggtccccgga tggtccggga
     8101 tgcaggaagc gatggttcat ggccgggcaa ggtaggcatt gggcacattg gccggttcat
     8161 acttaatccc gtaagcgtgc cggagcaatt tgctgaccgt ctccgcgatc acggtttcac
     8221 tccggtgcat ttggggcagc aggcccatca tggcataaga tatgggattc accgcgggaa
     8281 tgagggtctc ccactcatag cctttacccc agatcagtcc gggcacggtg acctggagct
     8341 tggcggcgcg gtccagcagg aaagtcagca tatccgccga ctgattcgaa ttctgattga
     8401 cggtatggaa gaccggggtt tgtccgccaa aaccgaattc atcaaaaccg gcggccgaat
     8461 tgatgtccgc accgtaccgg aagaggaggt ctgcacaggc cacatgattg aattctgcgc
     8521 aaaggtggag gaggctgact tcatgcatcg gggtataagc gcaccgcatg gaataggttt
     8581 tgtggatcag ggccccgtcg gcagccaata atttttccaa tgccggggca tcgtccagga
     8641 ggaccgccac cagtgccggg tcttctcctt tcaggccgaa atcgacaaag gcctttacgc
     8701 ataagctgaa cctcgggctt cgggtgtact cactggtgag ttcccggaaa aggggtatgc
     8761 cctggtagag gtcattgggg ctgatccccc gggcaaagga agaccggatg ccctccacat
     8821 cgtggagttc gatggctgtg atcagggagc caaggtcctt cataaggaaa aatagatata
     8881 aaaattaatg atgggcttac cgcttcctat tccgcagacc tgtacccatt gccatttcta
     8941 ttccttggtg ggaacgacca tcaccgggac tttggaatgg cgcacgacgg attcggcgat
     9001 gctgccgacc aggaaatgga ccagcccggt ccggccatgg gtgcccatga caatgaggtc
     9061 cgctccccag tcttccgcgg tttgcaggat ttcatcctgg ggatttcctt cgggggtgag
     9121 tttggacagc ctggacggat catgggactg ttccgccagc tggtcaatgg tttgctgggc
     9181 ttctttccgg agcatctctt ccgcctccat aggaaggatc cccgcgtcca cgttgcctat
     9241 ggctttcgcg gcatccacca caaagaccag ggccacggcc gcattggtct cctgggcgag
     9301 ctcaaaccct tttttggcgg catttacggc atagtcgctg ctgtcgactg caattaatat
     9361 tttggaaaat ggcatgttga ataattttga ttaaaggtaa ggtgaaaatg ggaagtagta
     9421 ggtaagttag caagttagca agttagcaag ttagcaagtt ggcaagttgg caagttagta
     9481 agttgggaag ttggcctccg ttctgagata ttttaattga aacgatcgca gtcagcatat
     9541 ggccccaacc aaacggttga tcaacttact gctttgccaa aagattcact tataaactat
     9601 cttctatttt tacgatcaga agccttcata tgaaatatat ttttgcctgt ttgttatgcc
     9661 tgaacctctt cggcgggtac ggtcaaaccc tgcccaccct gtttgaaacg agccaagccc
     9721 gggaaacgcc ggattactgg gaaatcatcc gctggtggaa aaccctggac cagcactcgg
     9781 acaaggtgca cctgcgtacc atgggcatga cagatgccgg ccaacccctg cacctggcgg
     9841 tggtctcgca agcgggggat acggattttg agcgcatgcg gaaggaaaac aaaagggtga
     9901 tcctggtcaa caacggcata cacccggggg agcccgacgg gatcgacgcg agcatgctgc
     9961 tggccaggga tattgctttt ggaaagatcc gcatgccgga ccaactcgtt cttgcgatca
    10021 tcccggtgta caatatcgga ggttgcctgg accggagtcc cgattaccgg gtggaccaga
    10081 acgggccgga ggcttttggt tttcgcggca attcccaaaa cctggacctc aaccgcgatt
    10141 ttatcaaatg cgattccaaa gaggcccgtg ctttcgcaga aattttccac ctggccgatc
    10201 cggacgtatt cctggacaac catgtgagta acggggcgga ttaccagcac atcatgaccc
    10261 tgatctgcag ccagcacaat aaactgggcg gaatcatggg tgaattcatg aataaggatt
    10321 ttgaaccggg catttatgcc tccatgaaaa agaaaggcta tgatctgatc ccctatgtca
    10381 atgcattcgg ggaccgcgtg gaccagggat ggccggaatt tatggacggg ccccgctaca
    10441 gcagcggtta tgcggcgctc tggcatagtt tttcctttgt tgctgaaacc catatgctca
    10501 aagcctatga ccagcgcgtc caggccacct acgaactcat gctcagtttt atggatttta
    10561 cctccggcca cagtaaacaa ataaaagaac tcagggaagc cacaaaaaat caggtaaaat
    10621 cccagcggga attcaccatt acctggaatc ccgaccggac ccgttcatcg gaacggcttt
    10681 ataagggata tgaatccggg tacaggaaaa gcgaggtctc gggcctggac aggctctatt
    10741 atgaccgcac cagaccctat gaaaagacca tccccttcta caatcattat ttacccggcc
    10801 agaaggtcca aaaagcttca gcctatgtga ttccgcaggg ctggtggaaa gtaatcgatt
    10861 tgctccggtt aaataaggtc aatatgatgc ccctcgaccg ggataccctc atggaggtgg
    10921 aggtttaccg gattgaagaa tacgaatcct cttcccggcc ttttgaaggc catcacatca
    10981 atactaaagt caggaccagc atctcagtgg gaaaaatgtc cttccggaaa ggggattatc
    11041 tggttcccct ggaccaggtt gccaaccgtt tcctggtgga ggtcctcgag cccgccgcgc
    11101 cggattctta ttttacctgg aattttttcg atgccgtcct tggacagaaa gagggataca
    11161 gcgcttacgc ttttgaagac attgccgcgg aatacctgca aaggcatcct gatctcaaag
    11221 aaaaactggc tgagcgaagg caatccgaca gcacctttgc ccggagcgcc cgggctcagc
    11281 tttattatgt ttatgaacat tcaccctgga ttgagccggc gtttatgagg tatccggttt
    11341 atagagtcaa tagtcagtag tcaatagtca gtagtcagga atcagacatc agtagtcagt
    11401 tagcaagtta gtgtgctcgt atctcgtatc tcgtttctcg catctcgcat ctcgcatctc
    11461 gcatctcgta tctcgtatct cgtttctcgt atctctggta ttttattcct ctatatcacc
    11521 agatcaccca aaggggtaag aatagtccag tctccgcgaa atagctttgg gtacacatcc
    11581 tgattttaaa tgaagtacct tctgatattc ttgtccctct tttctcaaat ccaggccttt
    11641 gctcaaaaaa ggttcgatat ccggatccag agcgagtcga ggttgcgcga agccattgtt
    11701 tcagtaccta ccacccctcc gccgcctggg ggctatccca tcgtttttat gtgtcacggc
    11761 acctccgggg aggcgtccaa tttttataac ccagtaggct ggaaggaatt gggacaggcc
    11821 gaaaatttta tcaccgtatt tccctcggca ctggtttggt gttaccttga tcagagagag
    11881 gataataaag agacccatct ttcaaaattt gtatgcgggg atttattgtc gcaggtgtgc
    11941 gaaaaggata tgaatgcgtt gatcagcgat atcctttttt ttaagaaaat aatccagctg
    12001 atctcggata ccctgaaggt ggaccaaaat aaaatcttta tgaccggatt ttccaacgga
    12061 agctccatgg ctcataagat ttccatggaa gccaccgatg ttttttccgc ttccggcggt
    12121 tcgagtggag ccttgcacgc cctggactcc accactccca ggtcacggag gatcccgatg
    12181 tggtatatgg tggggaccag ggatgaccgg tatttcagtg acctctttcc ggtggaactg
    12241 ccctttggcg gggattccat tttgatctat ttgcagggca gcattcgaag ggccctggtt
    12301 tgccagggac ttacggagga attcactaaa acggtaaccc cggtaagcca tatttatacc
    12361 tggaacacct gcaggaccgg cgaagcctgc gccccttacg tatttatgct caataaggat
    12421 cagacccatg aataccccaa cggccaaaac cacccggtag acgcccctaa aatcctctgg
    12481 aattttttta ataagcctcc ggccgtgaag atgaccactc ccatccggga aacaaaatcc
    12541 tccggtgtgg agctggtatt ctatcccaat cctgctacgg attttcttag tataaaattt
    12601 aaaggcctta aaccgggacc aggatacttg aagattgtaa atggatccgg caaggtcatg
    12661 gtccgacaaa atatattggc ggaaggcgcc gaccgtgtgg atctcagcgg atggccctcc
    12721 ggaatctatt atgcgatgtt cacctcccgg accggaagcc tggtaaaatt atttacgaag
    12781 gtttagagcg accggtttta cattcttcag cgatcattca ttttccggcc cgtaataaaa
    12841 aatgaccgca ccgctgccaa aggattttgt tttcaaaagt ttaagattta tccggccatc
    12901 tatactttta aacaaggaca tgccttttcc agcaatcacc gggtggacgc agagctggaa
    12961 ttcatcgatt aaatttaagt tcagggctgc cgcgatcagg cttgggctgc caatcaggat
    13021 gtctttgttc ccattcccgt ttgatgaggg aagggtggac ttgagctcca tgatttcttc
    13081 cctaaggcct tgttttgcca accttgaatt tctccaccag atatcttccg gttggagggt
    13141 gttggagaaa acaattttgg taatgcggtc gatgctgagt gcgaattcat ccaaggcttt
    13201 attgccggag ggatttttta tcaagcccgg ccagaattcc atcagctgat aggtgatcct
    13261 gccatagagg acggcgtccc cgctgtttaa cagttcctca taatgatggt ggacttcttc
    13321 atcaggattg acggaggtgt gatcgaaatt cccgtccagg gtcatattga tggcggcaat
    13381 gattctcctc atttggcggc tttttttctc ccgaaatata agcctcttcc cgttatcccc
    13441 tcttcatcat aacttacttc aaaacccgat tcgtggaatg ccttattcat ttcatcctgg
    13501 gtaaacaggg ccaattcgtg tttttctttg atatgtctta ctcctttttc cggagtcccg
    13561 actaaataat ggaaattcaa gagggacaaa cgacctttgg aagcgctccg gttcatgcgg
    13621 caaattttta aatgttctcc ttcgtaggtc agcatatgga tttttccatc ctgccaatga
    13681 tcaggagtca gccagggctc cacgaaaaca aggccatctt cttccaggtg ctggtaaaag
    13741 caagtcagcg cagacttcag ttttttatac gtttttacat aagcgatgga actgaagagg
    13801 caaaggatta cctcatattt tttccccagt ttaaaattta tcatgttttc aatataataa
    13861 tgccccgagg gatttttcct cttcgcgatt tcgataaatc cttcgtggag gtccagtccg
    13921 tcgatttgaa actcgtcttg caaatatttg tggtgttcgg cggtgccgca accgatatcc
    13981 aggaccgttt tggccccggg ccggatgcgt tttatccttt ggatcagatc tccggcttcc
    14041 ttctgataat ccttgaaacg ataaagcaaa tcgtagaact ctgcgctttc agtaaacatg
    14101 tcatttattt aagaatcgtg atccttttcc ttgattagga cccttccaag tatatgcgcc
    14161 ttattctgga attcacatgc tgcatcagac ctttattaaa tttcaataca ggtccaggtc
    14221 agatcttttg cccaaaggta aggagattgt ggtccggatc gagcacggag aattccctct
    14281 gcccccaggg tttgttctcg aggggtccgt tcgggtggat ggaaatgtgg cgatccaaga
    14341 gggaccggta cagttcatca atattatctg tccggatata gacctgcccg taattctcct
    14401 ttggatccag tccggggaat ccaaaaaagt ggatctggac ctggtctttt tccaccatca
    14461 ggtaagccgg gtggtccatg gatccgaatt cgttaaaacc aagccggtgg atataaaatt
    14521 cccgggtggc ggctttgtcg cgcatgggga gcttggggtg gatggcagtg agcatgggtg
    14581 aggtggggtt tgaagcaatt tgttaggtga aagtattcag tacttcattt ctatcaatac
    14641 cattgttcca gtcttttctt atctgttgcc ggcaggtatt ttttagcaag cgatatggga
    14701 gaagccaggt ggtgatcaat ccagaaagcc agcttccttt cgtctccctt ggattcaagg
    14761 gccagccaga tggctaccgt aaaagccccc catttcttat cgacggggcc cctggacaga
    14821 ttgcttacga tgctgttttt ggaggcttcg tcgggggacg gagaggatgc agccgccagg
    14881 agccggtccg taaaaacagg gtacaggctt gccaaggttt ttattgttat tcccggcaag
    14941 accccatctg cgggcagcca tttagaggag agagaacggt acatgccaat gccttcatac
    15001 aggcattccc aaaggatgct gtccttaaaa gttctgagct caaaatggtt taattccgcc
    15061 cattgtttgt gcagcaaatg ggtaaattca tgggcaaata tgcggtctat ccggccggag
    15121 ttttccggta tagcagcagg gccgtaattg ttatacaggg ccctgaggtc aaggcatact
    15181 gtattcaacc caaaagtaaa tccgtcgtcg tttccggaat ttcccataaa cagaatcgtg
    15241 gtatcggggg ctatacaatt tctgaagggg atggccaggg aatccaaaat caggccccaa
    15301 ccggcttttt tagattcaat caaatggatc cattcctttt gttcctggct taagggcagc
    15361 gccaaagcgg caaccgagtc cagcgccggt ttggacattc tagaagccaa tgccgatttc
    15421 catccggagg tcattttatc atcggcaaga caaagcaccg gaatgatgac tttaggtggt
    15481 gccggatggc tttgatggcc accttgtttt accagtcccc atccggtcaa aagggtcagg
    15541 gtaatccata aaaaaatagc attccccata atccgttata cctgcaaatt tattaattcg
    15601 gtgaaattat cggtcgaaac cgatccattt ggattatgtc gggcctttcc ttttaaccgc
    15661 ccggatgcac ataaatgccg gaaattcgtt caactccctg aaatgttttg gatccaatgg
    15721 ctcaaattcc ttgctgggtt tgggttcaac caactcgtca atgtaaaaac cattcccggt
    15781 caggggggat aaacatgcac tcaatggcct tctataacta tgtacttcca ccggggagcc
    15841 aaaacctctc caggtgcatt ttacgggttc aatttcaaag tatttttttg acttaaaaaa
    15901 attaaattca taaaagggat gttcaatgga aatcaccagc accccaccgg attttaatac
    15961 ccggtgaaat tctttaatgg tcatggacca gtcttcgata taatgcaagg ccagggcaca
    16021 taggacgata tcgcaggaat gatctccgat ccgttccagc ggcttggaca ggtcatgaac
    16081 aaaaaaggtg ccccgaccgg ggttcctttg aatagccagg tccaccattt tcgggctgag
    16141 gtcaaaaccg gtgaccaggg ctcccaggtc cattaagatt tcggcatata cgcccggacc
    16201 gcaggccgca tcaaggatcg ttttcccctt tacgtttggc agcaagcgta gggtattggg
    16261 ccggtcgtaa taggcgttat gaggtttatg cataaccagg gcgttgtagg cttcggccat
    16321 ttgttcataa gccgccagga tcttttcttt tgacattgga ttacccggcg ttttctaaaa
    16381 tatcggtggc ggtcttatgg ccgagctgtt cggccagccc gatcagcagt ccttcatggc
    16441 cccggaggta acaaagccgg taggcgtctt cgaaccggac cacttcgcct acgagttgag
    16501 caccgtgcct ggtgagcctg ctcaccaact catcgaggcg gtccaccgta aacatgatac
    16561 gaagataccc aagtgcgttc acaggagccg tccgatggtc ggatacggtg ggtgggtcga
    16621 gaaagcgtga aagttcgagc cggctgtggc catcgggggt caccatcatc gcaatttcaa
    16681 cggtttggga acccagcccg gttacccttc cggcccattc cccttcaacc agggctcgtc
    16741 cctccagttt caggccgatt tccgtaaaaa aggaaatggc cttgtcaagg gactccacca
    16801 cgacgccgac gttgtgcatt tgcaatattt tacttttagt catgaaccag ggctttgaat
    16861 gatttcttaa tgtaagaggt ctgtaagggc ggctccgttg gcggtgcctt ccatccttcg
    16921 tccgggtatg tccgccttca tggctttagt ttatacccta aaaaacttcc gagaaatttt
    16981 gctccattgg caattaaaaa aatgataagg aattcggccc atcggacacc ggctgcggtg
    17041 acaataatca ggtccagcaa aacataaatg aggggaaaca gaaaaaccag agtggtcaaa
    17101 ttcggatatt tctttttctt ccaataccgg acgaccagaa agaagatgat aaatccaaaa
    17161 atgcctgaaa tatagggggc ggaggacagg gcatgggcat catacactgc agcgtcttgt
    17221 ccgggattaa tgagatagga ataaacggcc acttcgagga atgaaataag cactaaggtg
    17281 atcatggtca gcatagccaa cccgattaat tttaaaaccg catatttttt catggccttt
    17341 ttttagtgtt taaaaaagta aattaaaaat actaccaggg tccatttttc ttaacctggt
    17401 aaaaccggga accgtcaaac cgaaaaaggc cttgccctgc accccaccac atatttcccg
    17461 cccggtcctg gtacatgctt tgaacacaac aggtcaatcc ttccgcggaa gtaaacacgg
    17521 taaaggcttt tggatggggt agtggaagag aagggtcaaa tctcgtggta caaccccggg
    17581 cagtgaccca aataatacct gatgcttcga taatgaggcc ccagaattcg gtcccgcata
    17641 atccgtcccg gggtgtgtat tccgtcaatg tatggccatc aaatttgcaa attccgtttt
    17701 tcatggtaaa ccacatattt ccggctttat cttctgccag accccctgca tagttatctc
    17761 ccagaccttc tttttctgca atattgctga ggtttacccc gtcataacga aagacccctc
    17821 tgttcgaaga agcaatccaa atattgcccc tttggtcttc caggataccg gtgacgttta
    17881 ttggagggag tgggttgaat aatgaaaagc tttggccgcc cacagtgtcc gcagaggggt
    17941 tatacttgaa aacaccccca tgggtgccaa tccatatcct gcctgattgg tcaacgagga
    18001 tgcctttccg tgtgatgtcg agttggctca gtccgtcttt ctggttgtaa tttctgaagc
    18061 ctgcctgcgc tgccactccg gtaggcaggg attttcctgg ggccccggcg gcaccgtatt
    18121 tatatacccc ctggtccgtc ccaaaccaga gattcccgtt tttatcttca ttgatggcat
    18181 aaaccgtgtt ctgatggaaa ccatcgggac tggaaaaata ggtcagggtt tttccatcat
    18241 accgcaccac gccctcgcct atggtgccaa accataaatg gcccctggaa tcctggaaaa
    18301 tgctccggat atactgactg accagggtcg tgtcaaaatc gggagccatg gcagcggccg
    18361 caaagttttt aattaccctt gggctggata tggatggaat ggggacttgc gaagaattta
    18421 ttatttcttg tcccttgcag gaagtaagaa tggacaaaaa gaaacaaaat attaatttgt
    18481 tcatggagtg ttataaactt gatggagcaa aaagcttccg ccctggctgc caaagataca
    18541 atgaactggt atcaaataat cccctgtgga cccggctcca cggtgggttt ctgcgttgaa
    18601 ttcaggcagg aatattttaa ctttattccc ggcagtttaa acggtacggg catgcttcat
    18661 taacgcatta gctccggaga ataaaagtta tatattcgaa aatgttaaaa cgggacttgc
    18721 attttatttt aaatggtttg tttattttat ccgctggttt tggttttata ctttgcactt
    18781 gcaccgatcc attaaaccat gaatcggtac ccaaagggga agcctatttg tcttttaccg
    18841 gtaatggggc atggtgttgg ttttcagatc cgagggccat atattttgag gggaagcatc
    18901 gaagaacttt tgccggatgg gttgatgact ccgggaatat tacggtgggt ttctatgacc
    18961 acgacttgaa acgtatcgaa acaacagtat tacataaaaa cttcgagaag gatgatcacg
    19021 accaccccag tttgttgatg gacagacagg gaaaactgat ggtgttttat tccaggcatg
    19081 cgagcccgga tccgatttac ctgctgaaag caaaacatgc cgaagacctt tcggaatggg
    19141 agccgccctt gagattggat ttaaacgact cgataacata cccgggaggg tccaatacct
    19201 atacttatac aaacctctgc cgattggccg acgaaaagga caggctttat cttttttggc
    19261 ggggcgcgga ttttaaaccc aatctttcaa cctcggatga tggaggtaaa gtatggagcc
    19321 cggggaaaat attcattctt cctgaccgga tttataaaga ccggagacct tatttaaaag
    19381 tcgcttcgga caacaaagcg gtcattcatt tcgcctttac ggacgggcat ccgaatgtgg
    19441 aaccggcaaa ctccatctac tatgtaaaat attccggcaa cgcgttctac aaagccggtg
    19501 gtgaaaagat agccgattgg tcagccctgc ccattcaacc taaatcggcg gacctggttt
    19561 atgatgcaac caaaacgaat gagaaagcct ggatatggga tgttgctgaa aatgaagaag
    19621 gtaagccggt aatcgtattt tccaccttcc ccaatgacac cagccacgtt tattactata
    19681 gtatctggga taataatcag tggatgaatc ataggctggt cgattcaggt ccatggtttc
    19741 cgcagacccc ggagggtgaa aaggagcggg aaccttatta ttcaggaggt atcgttttag
    19801 atcacgagga tccttccaac ctctatttat ccaggttaaa aaataaaaag ttcgaaattg
    19861 aaaaatggag cactgccaat aagggtgtgg attggaaggt ggaggagatt acccacaatt
    19921 ctgaaaacca taacgttcgg ccagtggtga tccggaatta ttcagaaccc gattccttga
    19981 aagtattatg gatgaacgtc gaaaaatacc ttcattacac ggattaccgg gcttcgatta
    20041 aaatgaatat aaggtaatgg ttcaggtttg aattgcgggg aaatttttgt ttggaaaatc
    20101 ggatttcgta aacatgaggt agagaaaaca gtgcgtctgc attatgcatt aggttgcccg
    20161 atggcctgag gcttaaaatc atttttcttg atatttgtaa aggtttgtca gacagttata
    20221 tagcagttat tttcaattgt atatctttgc cggaaaatgg agggtgaact ccctctttat
    20281 tcgatccatg tataaccagc gtacaaaaat caaggccctg ctcagctccg aagccaccca
    20341 ttttcagact accgtcatgg gctgggtgcg cagtttccgc aacaaccagt ttatcgccct
    20401 caatgacggg agcaccaata ataacctcca ggtggtggtg gaactgggca tgctggatga
    20461 aagcaacctc aaaaggatca ccataggcgc ctgcatccgg gccagcgggg aactcctgcc
    20521 ctccatggga aagggccaga aggtggaact caaggccagc gccatcgaaa tcctgggcga
    20581 cagcgatgcc gagacctttc ccatccagcc caagaggcat agtctcgaat tcctcaggga
    20641 gaatgcgcac ctgcgcttcc gaaccaatac tttcggggcc gtattccgag tgcgccacgc
    20701 cctggccttc gcgatccacc agttttttaa ttccaaggga tttgtatata tgcatacccc
    20761 cgtgatcacg ggcagtgatg ccgaaggagc cggggaaatg ttccgggtga ccacccttcc
    20821 gatcgacggc agcgccccgc gcaacgagga cggcagcatc aatttcaaat ccgacttttt
    20881 cggccggagc accaacctca cggtgagtgg ccagctggaa ggggaactcg ccgccatggc
    20941 cttcagcgaa atttatacct tcggtcccac cttccgggcg gaaaacagca atacgccccg
    21001 ccacctagcc gaattctgga tgatcgaacc cgagatggcc ttctacgacc tcgaagacaa
    21061 tgccaacctc gccgaagaat ttatcaaata tatcatccgt tacgccactg aatactgcag
    21121 cgaagacctt gccttcctgg ctcagcgact ggaagaggaa gaaaaagccc tgccccagga
    21181 aaaacgcagc ggcatgggcc tcctggaaaa gctggatttt gtggttaaga atcatttcga
    21241 acgcatcacc tatacccagg ccatcgacat cctccgggaa agcaaccaca acaaaaagaa
    21301 aaaattccaa taccccatcg accagtgggg catcgacctc cagagcgagc acgaacgata
    21361 cctggtggaa aaacacttcc agaagccggt gatcctcacc aactatcccg ccaaaatcaa
    21421 ggccttttac atgaggctcg atgaaaactg ccccccggac cgccaaaccg tcgccgccat
    21481 ggatatcctg gcccccggga tcggagaaat cgtaggcggc agccaacgcg aagagcgtat
    21541 ggaccacctg ctccgccgca tgagggaaat gcatatcccc gaaaaagaac tctggtggta
    21601 cctcgacacc cggcgcttcg gttcctgcgt gcattcgggt ttcggcctcg gctttgagcg
    21661 cctggtccag tttgtcacag gcatgaacaa tatccgtgat gtgatcgcct ttcctcggta
    21721 tccggggaat gcggagtttt aggagtcacc agtcagtatt cagtaatcag ctgccgatgc
    21781 attgcatcgg gatagcaatc tgccgtggct gacccaaaag agtttgctac tctaacttaa
    21841 aagcccgata ccctctgaaa cttcaaatac tctaacttaa tatctccgtg ccctccgcga
    21901 acttcgtgcg aaccataaac acatttttga tgccgaaggc atcaaaaacc tacccaagaa
    21961 ggcacacgga gatcaggaga gcacggagga gagttttttt ccagaattaa aactcctctg
    22021 aaactcaaat actccaactt aatatctccg tgccctccgc gaactccgtg cgaaccataa
    22081 acacattttt gatgccgaag gcatcaaaaa cctacccaag aaggcacacg gagatcagga
    22141 gagcacggag gagagttttt ttccagtatt aaaactcctc tgaaacgtat ccacccggcc
    22201 aagtacatat actcggatta gcggtatgaa taaagaag
//
LOCUS       DAOQXT010000079        22205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2699_length_22205_cov_6.683747, whole genome shotgun sequence.
ACCESSION   DAOQXT010000079 DAOQXT010000000
VERSION     DAOQXT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22205
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_22205_cov_6.683747"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1338
                     /locus_tag="PKM27_13470"
     CDS             217..1338
                     /locus_tag="PKM27_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNR08322.1"
                     /translation="MISRTSRIRKITLYPFDIRLKQPFVISLETITHARNVLVHIQCE
                     DPDLYGWGEASPYQTIQGETRDTQLMIGKGIAKLLVGQDTKEHELNARRINQSLAFNY
                     CIKSAFDMALYDILARLEGRPLYQYLNGKRKILRTDMTVGIAKPEEMAPSARAYIEQG
                     FTEIKIKLGKDPDEDIERMVRVREAAGPRIKLRIDANQGWDPSSALRVLQAIQPLDLE
                     HCEQPVAAKNLTGMQAVNRASPIPIMADESLFDAYDASTLIRMQACSQFNIKLGKSGG
                     IREALKILDLAEEAGIACQVGCFSESRLGITALCHLAMARDSIVYYDMDSPLMLSEDP
                     VIGGVVYQNTNEMHCPDLPGLGLDLDPLFLKNLTRIEID"
     gene            complement(1397..1720)
                     /locus_tag="PKM27_13475"
     CDS             complement(1397..1720)
                     /locus_tag="PKM27_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNR08323.1"
                     /translation="MRDITIHITDRQGITKDIEVPPDIDLNLMELAKANDLPVEGTCG
                     GMALCASCHMYIESDHKLHPPSYDEVVMLDQAFYVESNSRLGCQIRINKELDGLRVTL
                     APVAG"
     gene            complement(2593..3870)
                     /locus_tag="PKM27_13480"
     CDS             complement(2593..3870)
                     /locus_tag="PKM27_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HNR08324.1"
                     /translation="MKNNRRKFFGQGLAMAGLAASPWQTFTESYTQAIRRNEQYSLPS
                     DIKITDIKCGYIRGGHSLFVKIYTDQEVWGCGEGVDATPGTYHLVKMMGARLKGRSPL
                     NVHRLFEDIRRSGFFEGAQSGMFIAVLSAVESALWDLAGKILGLPVYQLLGGKFRDKI
                     RVYCDTALYQNQLPKPDDFAAAAKKAMEMGFNAIKFDIDQANDPNKYDRYNWTASPAE
                     LKRMVDQISAARQAVGPNIDICVDMHGRYDVTTGERVAKLLEPLNLMWLEEPVPAENP
                     EAYKKIADSTSTPICAGENHYLAHGFRPLLEMNAVDIVMPDLQKAGGLGEGQRIANLA
                     NLYYVPFAPHMVASFLGAMASCHCCASVPNFMILEWQIYFHTNPMFKEIVKFDGEMVD
                     KSFITLTEKPGVGVEINEEGMRKYATPGVPFFE"
     gene            complement(3870..4814)
                     /locus_tag="PKM27_13485"
     CDS             complement(3870..4814)
                     /locus_tag="PKM27_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HNR08325.1"
                     /translation="MKFTIWLALFPSLLLTNLSAQQISKDELLFLTPHWTGERFPDGR
                     PKVPDALLQRMKLVTLEEAWAVLRNENYKHQYDDGWQVINPDSVLVGRAVTATFMPGR
                     PDIQRVIDDLGHNKDGRVKSQNTWPIELLQKGDVYVVDQFGAHEDGPTIGDNLGNSIY
                     TKTGNGIIYNGAIRDINGLKELGGFTSYFRSYHPSHHLNNPDGELNTTLVGINTPTRI
                     GKVTVLPGDIVLGRDGGVIIIPPHLAEKVVKTSEIIRLRDMFSHQRLREQKYTAGQID
                     TRWSAEIEKDFSAWLNAHMDKLPVPKEQIQELLKARTW"
     gene            complement(4914..5810)
                     /gene="atpG"
                     /locus_tag="PKM27_13490"
     CDS             complement(4914..5810)
                     /gene="atpG"
                     /locus_tag="PKM27_13490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNR08326.1"
                     /translation="MAANLKEVRERIKSVINTQQITKAMKMVSAAKLRKAQQAIVQMR
                     PYTVKLNEILGHVIASLGGDAEFKLAKTREVKQALVIVITSSRGLCGAFNTNIIKTAV
                     SLIQESYGSHRKEGKLSILCIGKKGFDYFKKHYSDCQIIGDHVHLIESYKAEPTDKLV
                     ESLIDSFETALYDRVQVVYGRFKNAATQFPTSEDYLPVPKINLPHTGKKKLKADYLFE
                     PDKQTVLESLIPALLKIQMKRYIMDTSASEHGARMTAMDKASENAEELLKELRINYNK
                     ARQETITKELLEIVAGAAALGG"
     gene            complement(5817..7406)
                     /gene="atpA"
                     /locus_tag="PKM27_13495"
     CDS             complement(5817..7406)
                     /gene="atpA"
                     /locus_tag="PKM27_13495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNR08327.1"
                     /translation="MVSVKPDEISAILKQQLSGLKTQAELEEMGTVLQVGDGIARVYG
                     LNQVESGELVVFENGVQAVVLNLEEDNVGVVLMGPWDEIHEGSIVRRTGKIASINVGE
                     GYLGRVVDALGNPIDGKGPITGQTYAMPLERIAPGVIYRQPVNEPLQTGIKAIDSMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQKEFYEKGEPVFCIYVASGQKASTVANVART
                     LEEHGAMPYTVIVAASAADPAPMQFFSPFAGCAIGEFFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKINNNDDIAKNMNDLPESLVKAGLI
                     KGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESALFNAGVRPAINVGISVSR
                     VGGNAQIKAMKKVAGTLKLDQAQYRALEAFAKFGSDLDAATQASLEKGKRNVEILKQP
                     QYSPAPVENQIAIIYLGTSNLLNAIPVDKVNEFEEIFLSNLEKRFPETLNELRKGQLT
                     PDAENKLKSLASELAGLYRKK"
     gene            complement(7435..7995)
                     /gene="atpH"
                     /locus_tag="PKM27_13500"
     CDS             complement(7435..7995)
                     /gene="atpH"
                     /locus_tag="PKM27_13500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNR08328.1"
                     /translation="MSLARISSRYAKSLLDFALEQDKLERIYQDMLQLKAAVSVRDFV
                     LLCKSPIVQPGKKEQVFNILFKDRFDPVTFGFFNILLKKGREAYVPEIVDQFMSQYEA
                     IRKITRVTLSTATPLDEQVIRKIKEKLAAAKSTRENIELETQIKPALIGGFVLEFDDK
                     LYDASVAHRLQQMRKAFKENDSIKSI"
     gene            complement(7995..8507)
                     /gene="atpF"
                     /locus_tag="PKM27_13505"
     CDS             complement(7995..8507)
                     /gene="atpF"
                     /locus_tag="PKM27_13505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNR08329.1"
                     /translation="MIFLADFSPIKPEFGLFFWTLLFFLLFWGLIGKFAFKPISESLK
                     KRESDIQDAIDLAKKTREEMNNMKSENERVLAEAREERARILAEAKETKNAIVSEARD
                     KAKEEASKVIGSAMQDIENQKKLAMIEIKDQVSKMALDIAEKVIRKELQNNPDQQAYA
                     QKLVDDLNLN"
     gene            complement(8586..8792)
                     /gene="atpE"
                     /locus_tag="PKM27_13510"
     CDS             complement(8586..8792)
                     /gene="atpE"
                     /locus_tag="PKM27_13510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNR08330.1"
                     /translation="MTGSIAAIGMGLAVIGAGIGVGTIGGKAMEAIARQPEAVGDIRA
                     NMILTAALVEGAALIALLLSYLSA"
     gene            complement(8838..10133)
                     /gene="atpB"
                     /locus_tag="PKM27_13515"
     CDS             complement(8838..10133)
                     /gene="atpB"
                     /locus_tag="PKM27_13515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNR08331.1"
                     /translation="MIKFRQILICKKLIFTLLFSYGLLTGQSQQEGQGAQHTESADHA
                     AQDAHPGQEGHHEFDPASTAFHHISDQNIYSIGPFNIPLPCLLYTKGQGWDIFSSSRF
                     GFDGYGHGDGHKAYQQFVLHEGQVMRVQDPSFPTDEVEIQGFTAKEEADDKGKKIEIP
                     YVLYQGHEYRLDKKSTADGGLFGGGLTSFYDFSLGKNAISMLIVCLLLGWMFISIAGT
                     YRRNPGRAPKGLQNMMEMVILFIRDEVAKPFIGAKWEKFFPFLLSLFFFILGLNLFGQ
                     VPFFGGSNVTGNLSVTMVLALLAFILTTVNGNKHYWQHILWMPGVPAWVKTILTPVEI
                     LGIFIKPLTLMLRLFANITAGHMTLVVFVSLIFIFGKSGQNVPASLGASVGSGLLSIF
                     MMAIELLVAFIQAYVFTLLTASYLGAATEEHHHAEEKPH"
     gene            complement(10277..10915)
                     /locus_tag="PKM27_13520"
     CDS             complement(10277..10915)
                     /locus_tag="PKM27_13520"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNR08332.1"
                     /translation="MFFISVGAQKNVTYASSKDSDPKARQILKAVSEKIKTGKGVTFG
                     FSFVTQAAESAPVIQKGKAMILGNKFVLDFPEQQLTSDGTTLWVYLKKRNEVQIQDAA
                     GLAEDPISPYKLLKIHEAPELVYILAGESRVNGAVCDVVEFKPLNYDVEYFKIKAEID
                     RAQSQYRSITLYLKSGDSYVLNISSQANTPFADSLFAFDLKKYPGIRVEDLR"
     gene            11033..11914
                     /locus_tag="PKM27_13525"
     CDS             11033..11914
                     /locus_tag="PKM27_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNR08333.1"
                     /translation="MKSYRFILPLFLLLWSGHVFTQGQLMESLSLDSRYFGKKMKYSV
                     YLPSGYQASNRHYPVLYLLHGYTDNETGWTQFGEIERIANEMMENGKATPMIIVMPDG
                     GVSWYINNFDRSMPYEDYFFNEFLPFIESTYRIRKEKAYRAVAGLSMGGFGSFLFAIK
                     HPEVFAHCAPLSAAFIEESTSINSPQANYDRIFSVLYGPGLTGTDRINDTYKANDPFR
                     IVADTANTAKLKTVKYYIDCGDDDFLSSGNAAMHKLLREKGIPHEFRMRDGAHNWTYW
                     RHSIRDVLKIISASFHR"
     gene            complement(12137..12715)
                     /locus_tag="PKM27_13530"
     CDS             complement(12137..12715)
                     /locus_tag="PKM27_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08334.1"
                     /translation="MKTLFFAVLLTLLTAGTLSAADPAYRLTSYGHTLVLNTSNWNKA
                     DLLVQIQDQEGHILTTQEYKPKEAGRLKRFNLAQLETGAYTITITDQVRTCTQEFVLN
                     EKSMTIHPEVKTFIKPFITLDESKLKVNFLNLGKSATLKILGLQQETIYQTQIDQPAF
                     NKAYNLSNLAKGEYTVMVLQGDKYYSHPFELK"
     gene            12953..13834
                     /locus_tag="PKM27_13535"
     CDS             12953..13834
                     /locus_tag="PKM27_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR08335.1"
                     /translation="MRGAPGIRPVLELIELSFGQSFTYRKFEEEEPNLKPFWHYHPEI
                     ELVYVQEGSSKRHIGNHISYFNNGDLILLGPNLPHYGFTERLSGKRSEIVVQLKKDFL
                     GQGFFEKPELKSIDQLFERATSGLTFSGNTRETVGKKLDGLENETPFNRLIGLLEIFQ
                     VLAHSEEYQVLNVSGPSLLVKRSDNEKIEKVYDYVRAHFKEQISLDEISALVNMEVPS
                     FCRYFKRVTGKTFTAFVNDFRIVHACKLLSETNYSVSEICFESGFNNFSHFNRLFKMT
                     THTTPLAYRNESQTILL"
     gene            14064..16853
                     /locus_tag="PKM27_13540"
     CDS             14064..16853
                     /locus_tag="PKM27_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR08336.1"
                     /translation="MTYDQFTVKAQECILEAQRIAESLDQQIVDTPHLLKGIMLTESS
                     VYDFLLKKSGVNVASVKLELDKLIQTYPKVKGTDVKQALSNDANQALSRAKNMLKDFG
                     DDYISMELILLGILTGNDRTAQLLKNSGATEAGIKNAIKELRKGRSVKEQGAENTYNA
                     LGKYAVNLNERAENGELDPIIGRDDEIRRILHILSRRKKNNPILLGEAGVGKTAIIEG
                     IAWRIVRQDVPENLRTKQIFALDMAALVAGAKYKGEFEERLKAVIKEVKESGDRVILF
                     IDEIHTLIGAGGGQGAIDAANMLKPALARGELHTIGATTSDEYQKYFENDKALVRRFQ
                     TVFIDEPSIEETVSILRGIQDKYEVYHKINILDEALVAAAELSSRYIPDRHLPDKAID
                     LIDEAAAKLRLELDSVPDEVDELQRRVRQLEIEKEVIKRDHDANKMDVINKSLAEAKE
                     KLDIVSASWKNEKEIVDQIQNIKKTIEELQGQADKAERESNYEVVAKLRYGTLKEQEK
                     LLAEAEARLKALPEDNRFTNQEVTANDIAAVVSKWTGIPLQKMLQSERGKLLLLEEEI
                     GKRLIGQTAAVKAVSDAIRRSRAGLGDPNKPIGSFIFLGPTGVGKTELAKALAEVLFN
                     DEKAIVRIDMSEYQEKHAVSRLIGAPPGYVGYDEGGQLTEAIRRRPYSIVLLDEIEKA
                     HPDTFNILLQVLDDGQLTDNKGRTANFKNTIIIMTSNMGSEKILENFEDLEALGDKHR
                     TEIIETTKMEVFDLLKENMRPEFLNRIDDKIMFLPLTKQEIKKIAQLQLKSIRKNLSR
                     QNLSIELSENAVNLLVDLGYDPQFGARPLKRVIQNDIIDELAKFLLSGTFASGDTILV
                     DTDIKGFTFRSTLNPETGLLHSAMDSVDTDMKTKNSRDKKLHQLNKATREVEDAVKKV
                     KDGEQPEGQEEPSKN"
     gene            complement(16850..17647)
                     /locus_tag="PKM27_13545"
     CDS             complement(16850..17647)
                     /locus_tag="PKM27_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08337.1"
                     /translation="MKSLHLAIPAILLVVISTILLFTHHRMWSLGLAVGILALVGIYL
                     LKDLIDWKYYLKHPPRLDKPVLGWLERVPFYRQLSEGDKIRFQQRLALFMLAHEYRVQ
                     PLAHASDEEEIQAPEDLKAISSIPAVMLLFNQDEYLIENIERIVFYGHPFPSPSIKEL
                     HHSEWNEEDKVLIFSIPHLKKGVLEPFGYFDLGLYEWVRAIKTVPLPSYSWEQFEKEF
                     LIHKSQIGLALGIADPDLHAVRKVYELHRSYIQLAPEPPSGALPDRV"
     gene            complement(17714..18754)
                     /locus_tag="PKM27_13550"
     CDS             complement(17714..18754)
                     /locus_tag="PKM27_13550"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNR08338.1"
                     /translation="MIKRKLTLALILLSVFLSAQNDDKARVATLTKALNQERKEDGWM
                     SGGGFGLDMGQLLQINPKVGAGENRLGFGGAVTFFAKYKKNLLTWDNTGSFNMGIVKL
                     GTGILASTSAFADVKQPYQKSIDELRLSSRLGYKTSPNSKFSYAADFGFISQLTPTYR
                     GTDNKNYMSRFSTATITTDLVAKLFAPAYITFAPGIDYKPDPTLSFFLAPFGYKSVIV
                     ADRDLARLNIHGNKWTSIDDFKLSDSQLGGLLKGTYNAKYAKEKISYTSRLTLFSNYL
                     NQPGNVDLDWINEFGFNISKAFQISIWTNMFYDHDVKVQVTDYTEVGGVRGTGRRLSF
                     TEQLLIRYNMIF"
     gene            19152..20498
                     /locus_tag="PKM27_13555"
     CDS             19152..20498
                     /locus_tag="PKM27_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNR08339.1"
                     /translation="MTAQSKTDEDAWMIKNLFSKTLSEGKSHDWLRALTREASGRLAG
                     SAASMAAVELTRQIMDTLGFSRVYLQPCTVPHWVRGETEIVRIVNSPFIGSMDLKALS
                     LGNSTGSGPQGLTAEVIEVQSLDTLEKMGSSLLRGKIVFFNRPMDPTQVRTFAAYGGA
                     VDQRGRGPAMAARFGAAGALVRSMTTSLDDVPHTGATNFQAGERQIPALAISTLSAEL
                     LSRELKKGKVMVYIRNTSRMLAPKLSYNVIGEIRGSQFPEEIIAVGGHLDSWDVGEGA
                     HDDGAGCVQSIEVLYQLVKAGYKPKRTIRAVMFMNEENGLGGGLAYADSSNARKEKHI
                     LALESDAGGFTPRGIGFSAEKEVYETYFKKVNSWAGLLEPYDIKFVNGGGGADIGPLK
                     SQNGLLAGLLPDSQRYFDYHHTATDNFEAVNRRELFLGAAAMASLIYLVDKFGLKE"
     gene            20495..21031
                     /gene="hpt"
                     /locus_tag="PKM27_13560"
     CDS             20495..21031
                     /gene="hpt"
                     /locus_tag="PKM27_13560"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNR08340.1"
                     /translation="MTEVTVNGKVFQVLISRKELEEKIEALANLINSKYSGEEPPLLV
                     GILNGSFIFMADLVRRLHFPCTIQFIRIESYTGMSTSGVVKMEADVSPRWVGRHVIVI
                     EDIVDTGHTLSRFIPVLKSHQPASVFVCALLFKKEALEVSVQLDEYCFLIGRKFVVGY
                     GLDYDGYGRNLPEIYQLK"
     gene            21077..21150
                     /locus_tag="PKM27_13565"
     tRNA            21077..21150
                     /locus_tag="PKM27_13565"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21111..21113,aa:Asn,seq:gtt)
     gene            21214..22140
                     /locus_tag="PKM27_13570"
     CDS             21214..22140
                     /locus_tag="PKM27_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNR08341.1"
                     /translation="MSLLVIGTVAFDSLETPYGRADRVIGGSGTYISWAASYFHKPVM
                     LQSIVGGDFPEHEIEALKNRGVHTEGLEIVRDKKSFYWAGRYHDNMIARDTLVTELNV
                     LADFNPVLPESYRNPEYVMLGNLTPEIQLSVLSQMQRRPKLVVLDTMNFWMDIALDGL
                     KEVIRKIDVLTLNDEEARQLSGEFSLVRAAQAIHQMGPKYLIIKKGEHGALLFSEGHI
                     FFAPALPLAQVFDPTGAGDTFAGGFIGYLARTGVLTFENMKRAVIYGSAMASYCVEEF
                     SIDRLKTLDMGQITDRVKQFVELVRFDTDLKE"
BASE COUNT         5417 a         5626 c         5721 g         5441 t
ORIGIN      
        1 ggaaatgggg gtcaatgccc tgttttctca aagccatccc aaactggtgg ctgccaactc
       61 tcttttccag cacgcgggct tttcagtgag cgaccaattc ccaaaggcgg cagaggaatt
      121 ctccaatatg cccctgctgc tggtcaagga cctttaagcc actgcatgaa atataggttc
      181 agcatgtccc cgtttacatt caaatcctcc tgatccttga tttccaggac aagccggatc
      241 cgcaaaatca ccctatatcc ttttgatatc cggttaaagc agccttttgt catctccctg
      301 gaaaccatca cccatgcgcg aaacgtcctg gtccatatcc aatgcgaaga tccggactta
      361 tacggctggg gagaagccag cccctatcaa accatccagg gagaaacccg ggatacccag
      421 ctgatgatcg gaaaagggat tgcaaagctc ctggtcggcc aggacacgaa ggaacacgaa
      481 ctcaatgcaa gacggatcaa ccaaagcctc gccttcaact actgcatcaa gagcgccttt
      541 gacatggcct tgtatgatat cctggccagg ttggaaggcc ggccgctgta tcaatacctg
      601 aatggtaagc gaaaaatcct ccggacagac atgaccgtgg gcattgcaaa gcctgaagaa
      661 atggcccctt cggcaagggc ttatatcgaa caaggattta ccgagattaa aatcaaactg
      721 ggcaaggacc cggatgaaga tattgaacgg atggtccggg tccgggaggc tgccggaccg
      781 cgaatcaaat tgagaattga cgccaaccag ggctgggacc cttcctctgc cctgagggtt
      841 ttgcaggcta ttcagcccct ggacctcgaa cattgtgaac aaccggttgc tgcaaaaaac
      901 ctcacaggca tgcaagccgt aaaccgtgcc agtcccattc ccatcatggc cgatgaatcc
      961 ctgtttgatg cttacgatgc ttccaccctg atccggatgc aggcctgcag ccagtttaat
     1021 atcaaactgg gcaaatccgg agggattcgg gaggccttaa aaatactgga cctggctgaa
     1081 gaggcgggca tagcctgcca ggtcggttgt ttcagtgaaa gccggctggg cattaccgcc
     1141 ctctgtcatc tcgccatggc cagggactcc atcgtttatt atgacatgga cagcccgctg
     1201 atgttgagtg aagacccggt cattgggggc gtcgtatacc aaaacaccaa tgaaatgcat
     1261 tgccccgatc tcccaggcct tggcctggat ctggatccct tgtttttaaa aaatcttacc
     1321 cggattgaga tagactgaaa ctatgccggt ttatcgctaa cggaagaaaa cttaaaggcc
     1381 agcccataaa gctgcctcat cccgccaccg gggccagggt gaccctcagt ccgtccagtt
     1441 ccttattgat cctgatctgg cagcccagcc ggctgttgga ttcaacataa aaggcctgat
     1501 cgagcatgac cacttcatca taggatggcg gatgcagttt gtggtcgctt tcgatataca
     1561 tatggcagct tgcacagagg gccatgcccc cgcaagtccc ctccacaggc aggtcattgg
     1621 cttttgccag ttccatcagg ttgaggtcaa tgtccggcgg cacttcgata tccttggtga
     1681 tcccttgacg gtctgtaata tgtatggtga tgtcacgcat cccgcaattt gtccgatgtg
     1741 tttaaagcag gcaaaaatac ggaaaaccct tgtgggtaaa ggcgatactt ccacccagag
     1801 ccgatgaaat gatgtcctca aaacccagct attatagggt gaccaggaag ttaaaaaaat
     1861 ttgtttccca ccattggtgc agcagttgga tgaactgcaa ggtcaggagg gatcgcgcat
     1921 cctctgcgtc cttcgcgccc tcggcggtta tttcccgcaa agacaccaag ggcactaaga
     1981 tgaggccgga acggcctccg ctttgccctg ggtagctaat gttagcatga aaatatctat
     2041 gccgcctcca aattccctct gcgcatcctc tgcctccttc gcgctctcgg cggttatttc
     2101 ccgaaaagac accaaagcat ccctctgcgc atcctctgcg accatcgcgt cctttgcggt
     2161 tatttcccgc aaagatagca tctctttgtg catcctcagc gaccttcgcg ctcctttgca
     2221 gttatttccc gcaaaaacac cagcatctct ttgcccatcc tccgcgacct tcacgcccct
     2281 ttgcggttat ttcccgaaaa aaacctagca tctctttgcg catcctctgc gaccatcgcg
     2341 tcctttgcgg ttatttcccg ccaggacacc aaggacacca agaggggaaa catcggcctc
     2401 taatttgccc agggacaggt aatggtagaa tgaaattaac atgccgcatc taacctccct
     2461 ctgcgatctc caccccctct gaggttatca aattcgaaat acggtacccc ctgcgtacat
     2521 atgtctattc aaaaaatggt gcccccggcg tattatgtct attcaaaaaa tggcacgccc
     2581 ggggtataat gtttattcaa aaaatggtac gcctggcgtg gcatatttcc tcatcccttc
     2641 ttcattgatc tcgacgccca ctcccggctt ctcagtaagg gtgataaaac ttttatccac
     2701 catttcaccg tcgaatttga cgatttcctt gaacatcgga ttggtatgga aatagatctg
     2761 ccattccagg atcataaaat tgggtacaga agcgcaacaa tgacaggagg ccatggcacc
     2821 caggaaggaa gcgaccatat ggggtgcgaa gggtacataa taaaggttgg ccaggttggc
     2881 gatgcgctgg ccttcaccca ggccacccgc tttctggagg tccggcatca cgatgtccac
     2941 cgcgttcatt tccaacaggg gcctgaagcc gtgggcgagg tagtggtttt caccggcgca
     3001 gatgggggtg ctggtggagt ctgcgatttt cttataggct tccggattct cagcaggtac
     3061 gggttcttcc agccacatca ggttgagcgg ttccaacaat ttggcaaccc tctccccggt
     3121 ggtgacgtcg tagcgtccat gcatgtccac gcaaatatca atattgggtc ctacggcctg
     3181 gcgggcggcg gagatctggt ctaccatgcg ttttagttca gcagggctgg ccgtccagtt
     3241 gtaacgatcg tatttgttgg gatcgttggc ctggtcaatg tcaaacttga tcgcgttgaa
     3301 gcccatttcc attgcttttt tagcggcagc cgcaaaatca tcgggtttgg gcaactggtt
     3361 ttgatatagt gcggtatcac aatagaccct gattttgtcc ctgaactttc ctcccagcaa
     3421 ctggtagacc gggagcccca ggattttacc ggccaggtcc cagagggccg attcgaccgc
     3481 ggacaggacg gcaatgaaca ttccggactg ggccccttca aagaaaccgg aacggcggat
     3541 atcttcaaaa agtcggtgga cgttcagcgg gctcctcccc ttaaggcgag cgcccatcat
     3601 tttgaccagg tggtaggtac ccggagtcgc atctacccct tccccgcatc cccatacttc
     3661 ctggtcggta taaattttta caaaaagact gtgcccgccc cggatgtagc cgcatttgat
     3721 atcggtgatt ttgatatcgc tgggcaggga atattgttca ttccggcgga tcgcctgggt
     3781 ataactttct gtaaacgttt gccaggggga ggcggcaagg ccggccatgg caaggccttg
     3841 tccaaaaaat tttcttctgt tgtttttcat taccaggtgc gggcttttaa gagttcctga
     3901 atttgttctt tgggtacggg gagtttatcc atgtgtgcat tgagccaggc ggaaaaatct
     3961 ttttcaattt ccgcggacca acgggtatcg atttgcccgg cggtatattt ttgttcacgg
     4021 agtcgctgat ggctgaacat atcgcgcaaa cggatgattt cggaggtctt gaccactttt
     4081 tcagcaaggt gaggcgggat gattatgacc ccaccgtccc ggcccaggac gatatcgccc
     4141 ggcaggacgg tcacctttcc aatgcgcgtg ggtgtattaa tccccaccag ggtggtattg
     4201 agttctccat ccggattgtt caaatgatgg gaggggtgat aggacctgaa gtaggaggtg
     4261 aacccgccca gttccttcag tccgttgata tcccggatgg ccccgttgta gataatccca
     4321 ttgcctgttt tggtatagat cgaattcccc aggttgtccc cgatggtcgg accgtcttcg
     4381 tgggcgccaa actgatccac gacgtatacg tctcctttct gcaataattc gattggccag
     4441 gtgttctgag atttgaccct tccgtcctta ttgtggccga ggtcgtcaat gaccctctgg
     4501 atgtccggac gcccgggcat gaaggttgcc gtcaccgccc tgcctaccaa tacactgtcc
     4561 ggattgatca cctgccagcc gtcatcgtac tggtgtttgt agttttcatt ccgtaaaacg
     4621 gcccaggctt cttccagggt gacgagtttc atccgctgca acagggcgtc gggtaccttg
     4681 ggcctgccgt ccggaaacct ttccccggtc cagtgggggg tgagaaataa gagttcatcc
     4741 ttgctgattt gttgagcgga aaggttcgtc aacagcaagg aaggaaacaa agccagccaa
     4801 attgtaaatt tcatattgat ctgcaatttt tgtaaacgat tacattattg attaaagacg
     4861 gatcaataat aaagagttct tttgaaaata agacaggaat tttcagggtg gccttaaccg
     4921 cccagggcgg ccgcaccggc cacgatttca agcagttcct tggtgatggt ttcctggcgg
     4981 gccttgttgt aattgatccg cagttccttc agcaattctt ctgcattttc cgaagccttg
     5041 tccatggccg tcatcctggc cccgtgctcc gaagcggagg tatccatgat ataccttttc
     5101 atctggatct tcagcagggc ggggatcagg gattccaata cggtctgttt gtccggctca
     5161 aacaggtagt ccgctttcaa ttttttcttg ccggtatggg gcaggtttat cttcggtaca
     5221 ggaaggtagt cttcggaggt tggaaactgg gtagccgcgt ttttgaaccg gccgtaaacc
     5281 acctggacac gatcgtaaag agcagtttca aaggaatcaa tcaggctctc caccagttta
     5341 tcggtaggct cggctttgta cgattcgatc aggtgcacat ggtctccgat gatctggcaa
     5401 tcggaataat gttttttgaa ataatcaaag ccctttttcc caatgcaaag aatgctgagt
     5461 ttgccttctt tccgatgact gccataggat tcctggatca ggctgacggc cgttttgatg
     5521 atattggtgt taaaggcgcc gcacaggccg cggctggaag tgatcacgat gaccagggcc
     5581 tgctttactt ccctggtttt ggccagttta aattccgcat ctccgcccag gctggcaatt
     5641 acatggccca ggatttcatt cagctttacg gtatagggac gcatctggac aatggcctgc
     5701 tgggccttgc gcaatttagc agccgagacc attttcatcg ctttggtgat ctgctgggta
     5761 ttgattacgg attttatccg ttcccttact tcctttaagt tggctgccat ggagaattat
     5821 tttttacggt acaggccggc cagttcggac gccaggcttt tgagtttatt ttcggcatcc
     5881 ggggtcaact ggcctttacg gagttcattc agggtctccg ggaatctttt ttccaggttg
     5941 gaaaggaaga tttcctcgaa ctcattgacc ttgtccacgg gtatggcatt gagcaggttg
     6001 ctggttccca aataaatgat cgcaatctgg ttttccaccg gggccggaga atattgcggc
     6061 tgtttgagga tctccacatt ccttttccct ttctccaggg aagcctgggt agcggcatcc
     6121 aggtcggaac cgaatttggc aaaggcttcg agggcgcggt attgggcctg gtccagtttt
     6181 aaggtaccgg ccactttttt catcgccttg atctgggcgt tccctcccac gcgggatacc
     6241 gagatcccta cgttgatggc cggcctcacc cctgcgttga acaaggccga ttccaaaaag
     6301 atctgtccgt cagtgatgga aatcacgttg gtcgggatat aagccgatac gtcaccggcc
     6361 tgggtctcga tgatcggcag ggccgtaagg gagccgcccc ccttgatcaa tcctgccttg
     6421 accagggact cgggcaggtc gttcatgttt ttagcgatat cgtcgttgtt attgattttg
     6481 gcagctcttt ccaggagccg gctgtgcagg taaaaaacat cacccggata ggcttcacgt
     6541 cccgggggcc tgcgcaaaag cagggatact tcacggtaag ccacggcctg tttggaaagg
     6601 tcatcataga taatcagggc aggacggccg gtatccctga aaaattctcc gatggcacaa
     6661 cccgcgaaag gggaaaaaaa ttgcatggga gcgggatccg cggcagaggc cgccacgatc
     6721 accgtataag gcatggcccc atgttcttcc agggtcctgg ccacattggc gacggtcgat
     6781 gccttttggc cggaagccac atagatgcag aacacgggtt cgcccttttc ataaaattcc
     6841 ttctggttta tgatggtatc gatggcgatg gcggttttgc cggtctggcg gtcaccgatg
     6901 atcaactcac gctggccccg tccgatcggg atcatgctgt cgatggcctt gatgccggtt
     6961 tgaaggggtt cgttgacggg ctggcggtaa attacaccgg gtgcgatccg ttccaggggc
     7021 atggcataag tctgcccggt gatcgggcct ttgccgtcaa tcgggttgcc cagggcatcc
     7081 accaccctgc cgagataccc ttcccctaca ttgatggagg cgatctttcc ggtacggcgt
     7141 acgatgctgc cctcgtgaat ttcatcccag gggcccatca ggaccactcc gacattatct
     7201 tcctccaggt tcaataccac ggcctgaacg ccgttttcaa acaccaccag ttcaccggat
     7261 tcgacctggt tcaggccata gacgcgggcg atcccgtccc ctacctggag cacggtgccc
     7321 atttcttcga gttctgcctg tgttttgagc ccggacaact gctgcttcag gatcgctgat
     7381 atttcatctg gttttacgct taccataatc gttggatatt attgttgtga tgatttaaat
     7441 ggattttatg ctgtcatttt ctttaaaagc cttgcgcatc tgctgcaggc ggtgcgccac
     7501 gctggcatcg tagagcttgt cgtcaaactc gaggacaaat ccgccgatga gtgccggttt
     7561 tatttgggtt tcgagttcga tgttttcccg ggtgcttttt gcggcggcca gtttctcctt
     7621 gattttccgg atgacctgtt catccagcgg ggtggccgta ctcagggtga cccgggtgat
     7681 tttacggatg gcctcgtact ggctcatgaa ctggtccacg atctcgggga cataggcttc
     7741 cctgcctttt ttaagcagga tattaaaaaa tccgaaggtc accgggtcaa accggtcctt
     7801 gaacaggata ttgaaaacct gttccttttt accgggctgg acaatggggc tcttgcataa
     7861 aagcacaaaa tccctcacgg acacggctgc ttttaattga agcatgtcct ggtatatgcg
     7921 ttccagttta tcctgctcca gggcaaaatc caggagggac ttggcataac gggatgaaat
     7981 gcgggccagg gacatcagtt gaggtttaga tcatccacca gtttctgggc ataagcctgc
     8041 tggtcgggat tgttttgcaa ttctttgcgg atcacttttt cggcgatgtc cagggccatt
     8101 ttactgacct ggtccttgat ttcgatcatc gccagttttt tctggttttc gatatcctgc
     8161 atggcgctgc cgatcacttt ggatgcttct tctttggctt tgtcacgggc ttcgctgaca
     8221 atggcattct tggtttcctt ggcctccgcc aggatccggg ccctttcttc acgggcttcg
     8281 gcgagcacgc gttcgttttc ggacttcata ttattcattt cctcccgggt ctttttcgcg
     8341 aggtcaatgg catcctggat gtcgctttcc ctttttttga gcgattcgct gatgggctta
     8401 aaggcaaatt ttccgatcag gccccagaac agcaggaaaa acaggagggt ccagaaaaac
     8461 aaaccgaatt ccggtttgat gggactaaag tcagccagaa agatcatgat ctgtcttgtt
     8521 tttttaaaaa accacctggt aagaaccaat cgttcggacc aggcggtctg gttattggaa
     8581 tcggattaag cggaaagata agataagagc agggcgatca gggcagcacc ttccacaagg
     8641 gctgcggtca gaatcatgtt ggcccggata tcgcccaccg cttccggctg acgtgcgatg
     8701 gcttccatgg cttttccccc gatagtacct acgccgatgc cggcgccgat tacggctaaa
     8761 cccattccaa tagcggcaat tgaacctgtc ataatttgaa ctatttattt aaatgaatta
     8821 attttttcat ggtttgatca atggggtttc tcttcagcat ggtggtgttc ctccgtggcg
     8881 gcgcccaggt aggaggccgt cagcagggta aacacatagg cctggataaa agcgaccagc
     8941 agttctatag ccatcatgaa tatggacagc agtccggagc ccaccgaggc ccccagggag
     9001 gccggtacgt tctggcccga ttttccgaaa ataaatatga ggcttacaaa aaccaccagg
     9061 gtcatgtgac cggcggtgat gttggcaaac aaccggagca tcagggtaag gggtttgatg
     9121 aaaatgccca ggatctccac cggggtcaga atggtcttga cccaggccgg tacgccaggc
     9181 atccaaagga tatgttgcca gtaatgtttg ttgccgttga ccgtcgtaag gataaaggcg
     9241 agcagggcca ggaccatggt cacgctgagg ttgccggtga cgttggagcc gccgaagaaa
     9301 ggtacctggc caaagagatt gaggccgaga ataaaaaaga agagagaaag caggaagggg
     9361 aaaaactttt cccatttggc cccgataaac ggtttggcca cttcatcccg gataaacagg
     9421 atgaccatct ccatcatgtt ttgaaggcct ttgggggcgc gcccgggatt gcggcggtag
     9481 gtccccgcaa tcgagataaa catccagcct aaaaggaggc agacgatcaa catgctgatc
     9541 gcgtttttgc cgagggaaaa atcgtaaaag ctggtcaatc cgcctccgaa taatccaccg
     9601 tccgccgtac tttttttatc caggcgatac tcatggccct ggtacaggac ataggggatt
     9661 tctattttct tgcctttgtc gtccgcctct tcctttgcgg tgaatccctg gatttccacc
     9721 tcgtccgtgg ggaaagaagg gtcctgcacc ctcatcacct gcccctcatg aagcacaaac
     9781 tgctgatagg ctttatgccc gtcaccatgc ccgtatccgt caaaaccaaa acgggaggag
     9841 gaaaagatat cccagccttg ccctttggta tagagcaggc agggcagggg gatattgaag
     9901 ggaccgatgc tgtatatatt ctggtcggag atatgatgga aggccgtgga tgcggggtca
     9961 aattcatgat gaccttcctg cccggggtgc gcgtcctggg cggcatggtc tgcggattcg
    10021 gtgtgttggg ccccctgtcc ttcctgctgg gactgcccgg taagcaggcc gtaagaaaat
    10081 aataatgtga aaatcagctt tttacaaatc aggatttggc gaaattttat cattttctta
    10141 aaaatcgctg caaatgtacg cattttagga ttctccaata cagtttaaac cctgatttta
    10201 caagattccg acggtgtaca aagcccgcaa acggttgcgg caaggcgacg gaatggattt
    10261 ttggttatga aagggatcag cgcaggtctt ccacccggat acccgggtac tttttcaggt
    10321 caaacgcgaa aagactgtcc gcaaacgggg tattggcctg gctgctgata ttaaggacat
    10381 aagaatcccc gcttttcagg tagagggtaa tcgaccggta ttgggactgg gcgcggtcaa
    10441 tttcggcttt gattttaaaa tattctacgt cataattgag cggtttgaat tcgaccacat
    10501 cacaaaccgc cccgtttacc ctggattccc cggccaggat ataaaccagt tcgggcgctt
    10561 cgtggatttt aagcaattta tagggggata tggggtcttc agcgaggccg gcggcatcct
    10621 ggatctggac ctcattcctt tttttgaggt aaacccaaag ggtggtgccg tcggaagtca
    10681 attgttgttc gggaaaatcc aagacaaatt tattcccaag gatcatggct tttccttttt
    10741 ggatcacagg cgccgattcc gcagcctggg tcacgaagga aaatccgaaa gtcacccctt
    10801 taccggtttt gattttttca ctgaccgctt tgaggatttg cctcgctttg gggtccgaat
    10861 ccttgctgga ggcataggtc acgtttttct gcgctccaac agaaatgaaa aacaaagtga
    10921 aaaataaaga caaaaccaga cgcccgttca taaactaata atgcaaacgt aagaattcac
    10981 cgggaggttc ttaaattacg gttaaattgc cgcgctgcat aaattcagaa atatgaaatc
    11041 ttaccgattc atcctgcctt tattccttct tctatggtcc ggccatgtat ttacccaagg
    11101 acaactgatg gaaagcctca gcctggatag ccggtatttt gggaaaaaaa tgaaatattc
    11161 cgtatacctg ccttcgggat accaggcttc caacaggcac tatccggtct tgtacctgct
    11221 gcacgggtac accgacaacg agaccggatg gacccaattc ggagagatcg agcggatagc
    11281 caacgagatg atggaaaacg gcaaggccac ccccatgatc attgtaatgc cggatggcgg
    11341 ggtaagctgg tacatcaaca attttgaccg ttccatgcct tatgaagatt attttttcaa
    11401 cgagttcctt ccttttatag aatccaccta ccggatccgg aaggaaaaag cttaccgcgc
    11461 ggtggccggg ctgagtatgg gaggcttcgg ttccttcctg ttcgccatca aacatccgga
    11521 ggtctttgcc cattgcgcac ccttgagcgc ggcttttata gaggaatcca cctccattaa
    11581 ttctccacag gccaactatg accggatctt ttctgtcctt tatggtccgg ggctcacggg
    11641 gacggaccgc atcaacgaca cctacaaggc caatgatcct ttccggatcg tggccgacac
    11701 cgccaacacg gccaaactca aaactgtaaa atattatatt gattgcgggg acgatgattt
    11761 tctcagctct ggcaatgccg ccatgcataa attgctcagg gaaaagggca tccctcacga
    11821 attccgcatg cgcgacgggg cacacaactg gacctactgg aggcatagca tccgggatgt
    11881 cttgaaaatc atcagtgcta gcttccaccg gtgagtattc cataccgacg cgggacctcc
    11941 cttcgattga tttaaaatat ttaaaaagga gggggctggt ttagcccaat tatttggcca
    12001 acatcccgga tacaggcctt tccaaaaaaa aggggatcct ttgaccccct ttactaaaaa
    12061 caaggaaaac tttactataa actcgtattg tatgaataca aagaaggtgt tctcatactt
    12121 gcccgggacc tgaaacttat ttcagttcaa aggggtggct gtaatattta tccccctgga
    12181 ggaccatgac ggtatattcg cctttggcca ggttggacaa attgtaagcc ttgttgaaag
    12241 ccggctggtc gatctgggtt tgataaatgg tttcctgttg aaggccgagg attttaagcg
    12301 tcgccgattt tccaaggttg agaaaattca ctttcagttt ggattcatcc agggtgataa
    12361 acggtttgat gaatgttttg acttccggat ggatggtcat ggatttttca ttcaggacaa
    12421 attcctgggt gcaggttctg acctggtccg taatggtgat ggtataggca ccggtttcga
    12481 gctgggcaag attgaatctt ttcagccggc cggcttcctt gggtttgtat tcctgggtgg
    12541 tcagtatgtg gccttcctgg tcctggattt gcaccaggag gtccgctttg ttccaattgg
    12601 aggtgttcaa taccagggtg tgcccatagg aggtcaaccg gtaggcgggg tccgctgcag
    12661 aaagggtgcc ggcggtcagg agggtgagga ggactgcaaa aaaaagtgtt ttcataattc
    12721 gatttgtttt gttttaaaga tgattgggtt tgatgcaaca aagtaaagca ggagccgccc
    12781 cgccctgctt cctttgaatt ttccaatatt gttgttggat taactcctca ggaattggaa
    12841 ataaaaattc gtcctgaatt tttgcgggtc attttaagca aaggaagtgt taattgacac
    12901 ttgcttaatc ccgcaggtcc catatttgcc tgtgaatcaa atcccatcga atatgagagg
    12961 tgcccccgga atccgacctg tacttgagtt gattgagctg agcttcgggc aatcttttac
    13021 ttatcgcaag tttgaagaag aagagcccaa cctcaagcct ttctggcatt atcaccccga
    13081 gatcgagttg gtctatgtgc aggaaggttc ctccaaaagg catattggaa accacatttc
    13141 atactttaat aatggggacc tgatcctgct gggtcccaat cttccccatt atggatttac
    13201 cgaaaggctc tccggaaaac gctccgagat cgtagtccag cttaaaaagg atttcctggg
    13261 acagggcttt tttgaaaaac cggaattaaa atccatcgac cagttgtttg aacgcgccac
    13321 ttcgggcctg actttttccg gcaatacccg tgaaaccgtt gggaaaaaac tggacggcct
    13381 ggaaaacgaa acccccttca accggctaat aggccttctg gagatcttcc aggtcctggc
    13441 ccactctgaa gaataccagg tcctcaatgt gagcggccct tcactgctgg tcaaaagatc
    13501 ggacaacgaa aaaattgaaa aggtatacga ctatgtgaga gcccatttta aagaacaaat
    13561 atcgctggat gagatttcag ctttggtaaa catggaggtg ccttcttttt gccgctactt
    13621 caaaagggtg accggcaaga ccttcaccgc ctttgtcaac gatttccgga tcgtgcatgc
    13681 ctgcaaactg ctcagcgaaa ccaattattc ggtgtccgaa atctgctttg aaagcggatt
    13741 caacaacttc tcccatttca accggctttt caaaatgacc acccatacca ctcccctcgc
    13801 ctaccgcaac gaaagccaga cgatcctttt ataacaatag tcaatagtca atagtcagta
    13861 gtcagtagtc aggctgacct ctgacctctg acctctgacc tctgacctct gacctctgac
    13921 ctctgacctc tgatagttga tggctgatcg ctgcctaaaa gtcagttatt tccaattcct
    13981 ccctgtcaaa agttcttttt tccgtacgaa ttcaggtttg gtcggcaaat tgataggtag
    14041 taccttgaaa taatatttaa aaaatgactt acgatcagtt taccgtaaaa gcccaggaat
    14101 gcatcctgga ggcgcagcgc atagcagagt ccctcgacca gcaaatcgtg gatactcccc
    14161 atttgcttaa aggcattatg ctgactgaat ccagcgtcta tgatttcctg ctgaaaaaga
    14221 gcggggtaaa cgtcgcctcg gtaaaactgg aactggataa actgattcag acctatccca
    14281 aagtgaaagg cacggacgtc aagcaggccc tgtccaatga tgccaaccag gccctttccc
    14341 gggcaaaaaa catgctgaag gattttggcg atgattacat cagcatggaa ctcatcctgc
    14401 ttggcatcct caccggcaac gaccggaccg cccaacttct gaaaaacagc ggggccaccg
    14461 aagccgggat aaaaaacgcc atcaaggaac ttcgcaaagg ccgctcggtc aaagaacagg
    14521 gggctgaaaa cacctacaac gcactgggca aatacgccgt caacctgaat gaaagagctg
    14581 aaaacgggga actggatccc atcatcggcc gcgatgacga gatccgccgg atccttcata
    14641 tcctgtcccg caggaagaaa aacaatccca tcctgcttgg tgaagccggg gtgggcaaga
    14701 cggccattat cgaaggcatc gcctggcgga tcgtccgtca ggatgtgccg gaaaacctca
    14761 ggaccaaaca aatctttgcc ctggacatgg cggcgctggt cgcgggcgcc aaatacaagg
    14821 gggaattcga agagcgcctg aaagcggtga tcaaggaggt caaggaatcg ggggaccgtg
    14881 tgatcctgtt tattgacgaa atccataccc tcatcggggc cggcggcgga cagggggcga
    14941 tcgatgccgc caacatgctc aaaccggcgc tggcccgcgg cgagctgcat accatcggag
    15001 ccaccacttc ggatgaatac cagaaatatt ttgaaaacga caaggcactg gtacgccgct
    15061 tccagaccgt attcatcgat gaacccagca tcgaagaaac cgtatccatc ctccgcggaa
    15121 tccaggacaa atacgaagtc taccacaaga tcaacatcct ggatgaagcc ctggtggctg
    15181 cggcggaatt gtccagccgc tatatcccgg accgccacct cccggataaa gccatcgacc
    15241 tgatcgacga ggccgcagcc aaactccggc tcgaactgga cagtgtcccg gatgaagtgg
    15301 atgaacttca gagaagggtc agacagctgg agattgaaaa agaagtgatc aaaagggacc
    15361 acgacgccaa taagatggat gtgatcaaca agtccctggc tgaggctaaa gaaaaactgg
    15421 acatcgtcag cgcctcctgg aaaaacgaaa aagaaatcgt cgaccagatc caaaacatca
    15481 aaaaaactat tgaggaactc cagggccagg ccgataaggc cgaacgggag agcaattatg
    15541 aggtggtggc taagctccgt tacggaaccc tcaaagaaca ggaaaaattg ctggccgaag
    15601 ccgaagcccg attgaaggcg cttcccgaag acaaccggtt caccaaccag gaagtcaccg
    15661 ccaacgatat tgccgccgtg gtatctaaat ggaccggcat cccgctgcag aaaatgctgc
    15721 aaagtgaaag gggaaaactc cttctactgg aagaagaaat cggaaaacgg ctcataggac
    15781 agaccgccgc cgtcaaagcc gtgagcgatg ccatccggcg cagcagggct ggcttgggag
    15841 atccaaacaa accgattggc tccttcattt tcctgggccc caccggcgtg ggcaaaaccg
    15901 agctcgccaa agcgctggct gaggtgttgt tcaacgatga aaaagccata gttcgcattg
    15961 acatgagcga ataccaggaa aaacatgcgg tatcccggct gattggggct cctcccggat
    16021 atgtgggtta tgatgaaggc gggcaactta cggaagccat tcgccggagg ccttattcga
    16081 tcgtgctcct cgatgagatt gaaaaagccc atcccgatac gttcaacatc ctgctgcagg
    16141 tcctggacga tgggcaactg accgataata aaggccggac ggccaatttc aaaaacacca
    16201 tcatcatcat gacctccaac atggggtctg agaaaatcct ggaaaatttt gaggaccttg
    16261 aagccctggg cgacaagcac cggacggaga tcatcgagac gaccaagatg gaagtttttg
    16321 acctcctcaa ggaaaacatg aggcccgaat tcctcaaccg gatcgatgac aagatcatgt
    16381 ttttaccgct caccaagcag gagatcaaaa agatcgccca gttgcaattg aagtccatcc
    16441 gcaaaaacct cagccggcaa aacctgtcta ttgaattatc ggaaaatgcg gtgaacctcc
    16501 tggttgatct ggggtacgac ccgcagtttg gggccaggcc gttgaagcgg gtgatccaga
    16561 atgatattat tgacgaactg gccaaattcc tgctttccgg gacctttgct tcaggggata
    16621 cgatcctcgt agatacggat atcaaaggct ttactttccg atcaacccta aacccggaaa
    16681 caggtctcct tcacagcgcc atggacagtg tggacaccga tatgaagacc aagaattcaa
    16741 gggataaaaa acttcatcag ctcaataaag ctacccgcga agtggaagac gccgtcaaaa
    16801 aagtaaagga cggggagcaa cccgagggcc aggaggaacc ttccaaaaac taaacccggt
    16861 ccggcagggc cccggaagga ggttccggcg ccagttggat atagctgcgg tgcaattcgt
    16921 aaacctttct cacggcatgc agatcggggt ccgcgatgcc taaagcaagg cctatctggg
    16981 atttatgaat gagaaattcc ttttcaaact gttcccaact gtaggagggc aggggtacgg
    17041 tttttatcgc acgcacccat tcataaaggc ccaggtcaaa ataaccgaaa ggctccaaaa
    17101 cccccttttt gagatggggt attgaaaaaa tgaggacctt gtcttcttcg ttccattcgc
    17161 tgtggtgcag ttcttttatg gaaggactgg gaaagggatg gccgtagaag acgatgcgtt
    17221 cgatgttttc gatcaggtat tcgtcctggt tgaaaagaag catgaccgcg ggtatgctgc
    17281 tgatcgcctt gagatcttcc ggggcctgga tctcttcctc atccgaagca tgggccaggg
    17341 gctgcacccg gtattcatgg gcaagcataa ataaggccag gcgctgttgg aaccggattt
    17401 tatccccctc ggaaagctgg cggtaaaaag gcaccctttc cagccagccg agtacaggtt
    17461 tatccagcct ggggggatgt ttgaggtagt acttccagtc gatgaggtct ttgagcaggt
    17521 aaatgccgac caaggccaga atccccacgg caaggcccag ggaccacatc cggtgatggg
    17581 taaagagcaa aatggtgctg atcaccacca ggagaatagc cggaatggcc aggtgcaagg
    17641 atttcatggt tcagttaaaa acgggaaaaa ccccggattg tatatgtcac cgcctccccc
    17701 tgatcaagtg cgtttaaaaa atcatgttgt accggatcag taattgttcc gtaaagctca
    17761 gcctcctgcc ggtgccgcgc acccctccca cctcggtgta atccgtgacc tgcactttca
    17821 catcatgatc gtagaacata ttggtccaga tggaaatctg gaaagccttt gaaatattga
    17881 agccaaactc gttgatccaa tccaggtcca cattacccgg ctgattgagg taattggaga
    17941 agagggtcag cctgctagta taactgattt tttcctttgc atatttggca ttgtaagttc
    18001 ctttgagcag gcctcccaac tggctgtcgg aaagtttaaa atcgtcgatg ctggtccatt
    18061 tattcccatg aatgttcagc cgtgcaaggt cccggtcggc cacaatcacg gacttgtacc
    18121 caaaaggagc aaggaagaag gaaagtgtgg gatcgggttt atagtcgatt ccgggggcaa
    18181 aggtgatgta ggccggggca aacagtttgg ccaccaaatc ggtggttatg gtggcagtgc
    18241 tgaacctgga catgtaattt ttattgtccg taccccggta agtcggggtc agctggctga
    18301 taaacccgaa atcagcagcg taggaaaact tgctgttggg gctggtttta taacccagcc
    18361 tggaactcaa ccgaagttcg tctattgatt tttgataggg ttgcttgacg tccgcaaagg
    18421 cggaggtgct tgcgagaatt ccggtgccga gtttgacgat acccatattg aaggaaccgg
    18481 tattgtccca ggtgagcagg tttttcttgt atttcgcaaa aaacgtgacc gcacctccga
    18541 aacccagcct gttttcaccg gcgcccactt taggattgat ctggagcaat tggcccatat
    18601 ccaggccaaa acctcccccg gacatccagc catcttcctt tctttcctgg ttgagggcct
    18661 tagtgagggt ggccaccctc gccttatcat cgttttgggc tgaaagaaaa acgctcagga
    18721 gtatcagggc aagggtgagt tttcttttta tcattttggt caaaaatttc ctgcaaagtt
    18781 atgggtacca tccatattat tcaggtggcc gctcgtattg tgccttatgt cttttgggga
    18841 cctgcggccg ggctgttgct ttgaaaagac cgccacaagg atctgggaac agggatggtc
    18901 agctttccaa cagagattac gcggtttcga ttgtaaacgg ttacaataac cttgaaataa
    18961 aaagaggtaa tgcaattttc atcacattac ctcgacccta accaaatgaa accaaattaa
    19021 tttttatttc taagataact tgatatcttg atgacataaa aatatttcaa ttcaagccaa
    19081 gatcatatat gcttcgggat caaaaaatta tattaattgt cattttcaac ttcttatttt
    19141 ggggaccatt tttgactgct cagtcaaaaa cggatgaaga cgcatggatg atcaaaaacc
    19201 tcttttcgaa aaccctctcg gaaggcaaat cccacgactg gctccgtgca ctcacccggg
    19261 aagcctccgg gaggcttgcg gggtccgcag cttccatggc cgccgtggaa ctgacccggc
    19321 agatcatgga tacccttggc tttagccggg tctatcttca gccatgcacc gttccgcatt
    19381 gggtccgggg agagactgaa atcgttcgca ttgtaaattc cccctttatc ggcagtatgg
    19441 acctgaaggc cctgtccttg gggaattcaa ccggctccgg tccacaggga ctgactgccg
    19501 aagtcatcga ggtgcagagt ctcgacaccc tggaaaaaat gggcagcagc ctcctccgcg
    19561 gcaagattgt ctttttcaac cgacccatgg acccaaccca ggtccggact ttcgccgctt
    19621 atggcggtgc ggtggatcag cgcggccgcg gacccgccat ggctgcccgt tttggagcgg
    19681 ctggggccct ggtccggagc atgaccacca gcctcgacga tgtaccccat accggtgcta
    19741 caaactttca ggcaggggaa aggcaaatcc ccgcactggc catcagcacc ctgagtgcgg
    19801 aattgctcag ccgggaactt aagaaaggga aggtcatggt ttatatcagg aacaccagcc
    19861 ggatgctggc cccaaaatta tcctataatg tcatcggaga gatcaggggt tcccagtttc
    19921 cggaggagat cattgccgta ggcggccacc tcgattcctg ggatgtcggc gaaggggccc
    19981 acgatgacgg ggcaggttgt gtccagagca tcgaagtgct gtaccaactt gtaaaagcag
    20041 gctacaaacc aaaaaggaca atccgggctg tgatgtttat gaatgaagaa aacggcctcg
    20101 gcgggggcct cgcttatgcc gattcctcga atgcaagaaa ggaaaaacat atcctcgcgc
    20161 tggagtccga tgccggcgga tttaccccgc ggggcattgg tttttccgcc gaaaaagagg
    20221 tttatgaaac ctatttcaaa aaagtgaaca gttgggcagg cttgctcgag ccctatgata
    20281 tcaagtttgt caatggcgga ggcggcgcgg atatcggccc ccttaaaagt cagaatggcc
    20341 tgctcgccgg attgttgccc gacagccagc ggtatttcga ctaccaccac acggcaaccg
    20401 acaattttga agcggtcaac cgcagggaat tgttcctggg agcggcagcc atggcttctc
    20461 tgatttacct ggtcgataaa ttcggactga aggaatgacc gaggtcacgg tcaacggcaa
    20521 ggtctttcag gtcttgattt ccaggaagga actggaagaa aaaatcgaag ccctggcaaa
    20581 cctgatcaat tcaaaatatt cgggagaaga gcctccttta ctggtcggga tcctgaacgg
    20641 ttcctttatt tttatggccg acctggtacg ccgacttcat tttccctgca ccatccagtt
    20701 tatccggatt gaatcctata cggggatgtc caccagcggg gtggtcaaga tggaagccga
    20761 tgtcagcccc agatgggtgg gcaggcatgt catcgtcatc gaggatatcg tggacaccgg
    20821 ccacacgctc agcagattta ttccggtcct aaaatctcac caaccggctt ccgtttttgt
    20881 ttgcgccctg ctgtttaaaa aagaggcgct ggaagtttcg gtccagcttg acgaatattg
    20941 tttcctgatc ggccgcaaat ttgtggtcgg ctatggcctg gattacgacg gttacggccg
    21001 gaacctgccc gaaatttatc aattaaaata gtgggcactt gagtataaat cattaatttt
    21061 gcccacccaa aagggagcct ccatagctca gttggttaga gcgacggact gttaatccgc
    21121 aggtcccagg ttcaagtcct ggtgggggcg caaaagaaag ggcattcctt accgggttgc
    21181 ccttttgttt tttaacttct aatcctttag tgcatgagtt tattggtaat cggtactgtg
    21241 gcctttgaca gcctggaaac cccttatggg agagccgacc gggtgatcgg cggttcgggc
    21301 acctatattt cctgggctgc ttcgtatttc cacaagccgg tgatgctgca aagcattgtc
    21361 gggggggatt tccccgaaca cgaaatcgag gccctcaaaa acaggggggt gcatactgaa
    21421 gggctggaga tcgtacggga taaaaaatcc ttctattggg ccgggcggta ccacgacaat
    21481 atgatcgcac gcgatactct ggtcaccgaa ctcaatgtgc tcgccgattt taatcctgtc
    21541 ctgcccgaat cctaccgcaa tccggaatat gtgatgctgg gcaacctgac gcctgaaatc
    21601 cagctcagcg tgctcagcca gatgcagcgc cggccaaaac tcgtggtcct ggacacgatg
    21661 aatttctgga tggacatcgc cctcgacggg ctcaaggaag tgatccggaa aattgatgtg
    21721 ctcaccctga acgacgaaga ggcccgccag ctctccggtg aattttcact ggtccgggcc
    21781 gcacaggcca tccatcaaat gggcccaaaa tacctgatca taaaaaaagg ggaacacggc
    21841 gccctgcttt tttccgaagg ccacatcttt ttcgcgccgg cccttccgct cgcccaggta
    21901 ttcgatccaa cgggcgccgg cgataccttc gccggtggtt ttataggcta cctcgcccgg
    21961 acgggagttt tgacttttga aaatatgaag cgggcggtga tctatggttc cgccatggct
    22021 tcatactgtg tggaggaatt ttcaatcgac cgcttaaaaa cactggacat gggccagatc
    22081 accgatcgcg tcaaacagtt tgtggaactg gtgcggtttg ataccgacct gaaggaatag
    22141 tggtcagcaa tcagtagtgc ttgttgcaca actctgaaaa taatcatatt aaaatatttg
    22201 ttata
//
LOCUS       DAOQXT010000080        21322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2836_length_21322_cov_6.517609, whole genome shotgun sequence.
ACCESSION   DAOQXT010000080 DAOQXT010000000
VERSION     DAOQXT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 21322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21322
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2836_length_21322_cov_6.517609"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKM27_13575"
     CDS             <1..298
                     /locus_tag="PKM27_13575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNR08342.1"
                     /translation="AYIFFYYLVWEFYFRGFLLFGLAKIYGPAAAILIQTISSCLVHL
                     GKPEGETLGSVAAGLLFGLLALRTGSIWYGWLIHFSLGVMTDFFILRNAGVSLF"
     gene            312..1310
                     /locus_tag="PKM27_13580"
     CDS             312..1310
                     /locus_tag="PKM27_13580"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR08343.1"
                     /translation="MNILITGANGFIGSRLAMRLTEMGHKIRGLVRETSRLDKLNGLE
                     LELAFGDITVPETLVKPMQGMDLVIHTAGAVTDWGPLSYFEQQNVDGLQQVFKAAEAA
                     GVGRFVHIGTVAIYGFGRIGVKEDDPFAESHVCYCISKRKGVEWLKRLAGHTKMEMTI
                     VHPGNVFGPGDDKFILPFLQLLDRNRFVWVNGGRAWTCPTYIENLVQGIALASFHPAA
                     AGETFIITDGLEMDWKTFAGALFRGLGKKPVTLSVPRPIVYSVAFMLETVYKLIHSRQ
                     APALTRYRIDNAGLDYHFSIEKAQRLLGYLPPVDFQASIDRTVQWYGERKLIKLKN"
     gene            complement(1321..3654)
                     /locus_tag="PKM27_13585"
     CDS             complement(1321..3654)
                     /locus_tag="PKM27_13585"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08344.1"
                     /translation="MKRILLIAILILPALSAVFSQPETNQRPSTGREKRIAFIQHERL
                     LASSPYQEVKWKLLNPANISGRSTDAWGIPGNKKIMYAGFATGGLWKTVDSGKTWTPL
                     MDRQATQAIGNMAIAPSNPNVIYVGTGEANIFRASLAGTGVYKSTDGGNFWNHLGLEN
                     TNTISRILIHPTDPNIVYVAAGGNEWSRNEDRGVYKSTDGGLNWKKILYVNDKTGAND
                     LLMDPSDPNTLYASMWTRIRKRWSDPVPEDGDHIYKSTDAGETWKIINNGLPDTKETG
                     RIGLANTPANPNVLYAFVDNHGKKRDPNPGEQDSYNRPKVVVIKGAEVYKSTDKGESW
                     KRMSEHDDFMEFFSGTYGWVFAQVRVSPTDENKVYLMGLDLTMSADGGKTWKSLTKGP
                     KGEWGRVHGDHHGLWIDPTDPNYLINANDGGVNVSYDGGLTWKGFYHELPTTQFYNVT
                     YDPRTPFYIYGSIQDEGSVRMLSTTTYDPADRSTGKFDWIPGGEGTRIAIDQKEPDIL
                     YSSSYYGHIMKGNLKRLGKKGASESIDPPVSLLGEEHRGEWLAATIISPHDGTTLYHG
                     LQYVLKTTDKGKTWEKISPDLTQNNRNKIGTLPYRINYQTISALAESPKKKGLLYAGT
                     DDGKAHFTTLEGNGKWTEISKGLLEDAAVSSIEPSRFEDATVYLSQSDRREDNITPLL
                     FKSSDYGRNWIKINSNLPASPVNIIREDPDNKDILYAGTDMGIYRSTDGAKTWNAMHG
                     NLPASVSVQDLFIHPTTKQIVIATYGRGVWILDRPNP"
     gene            complement(3771..4343)
                     /locus_tag="PKM27_13590"
     CDS             complement(3771..4343)
                     /locus_tag="PKM27_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08345.1"
                     /translation="MSLLQSLNSRIVLEKAARWYVFIMLNVYGWGKMLGGQFYRRGHL
                     PAEVAGQTLGEANAFDLAWTFMGYSFNYILFVGILQVAGAWLLIWERTKLIGAFILLP
                     ILANVVVFDLLFMDKHGATASALLYFSLVLIILFLNRKKILKALSGLMSLDHTLSPGT
                     RMAWLQILAALLTVAVLFGLDQFFVNLFGH"
     gene            complement(4365..7619)
                     /locus_tag="PKM27_13595"
     CDS             complement(4365..7619)
                     /locus_tag="PKM27_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNR08346.1"
                     /translation="MDGKIKTLFVLAVLIGLWPSCKEKKQATLYTSVPADSSGIRFSN
                     LLNEKGPVNILEYLYYYNGAGVAAGDLNNDGLPDLYLASNETGNKLYLNKGNFRFEDI
                     TEASGTACENQWNTGVSMADVNGDGWLDIYVCAIGDYKSFNGQNRLYINNRDLTFTEL
                     SNQYGLDFKAFSTQAAWLDYDRDGDLDMYLLCHSIHSVETYRDTSIRRQADPQKGDRL
                     MRNDGMKFTDVSPEAGILSSPLGYGLGVGVSDFNADGWPDILIGNDFHENDYLYINRQ
                     DGTFRQSQTLSFGHTSTFSMGSDIADINDDGFTDIVSLDMKPESEEVLKNSVGADPYD
                     IYQYKADFGYHFQYPRNTLQLNTFIRPDGLPIFSEIGQLSNISRTDWSWTPLLADFDN
                     SGSLDLFVANGIAHRPNDLDYLKYLAAPSASDTSDPGSLIQKMPDGRVRNYFFSQESP
                     LLFTDRSALWNPEGPDLSTGAAYADFDLDGDLDLVINRINEPASLLRNETQPPGHSLR
                     IKLEGQGLNPFAVGARVTLFQGDQKKTREIFPVRGFQSSSDYTVHFGLGSHTGIDSLL
                     IRWPDGSLQRIEAVQPDRLNTIKQQDRKEGYAAPVSRETLFKAADPGIAYLHVENPYN
                     DFEREKLLLYKLSTQGPGSAVADINGDGLEDLWIGGARGQAGITYLQTPSGSFTALAQ
                     NSLDLHASFEDVDGSWIDADQDGDPDLAVASAGYQMDQPALLVDRLYLNDGKGNLTFR
                     EDGFPAQAHMSACIAAHDLDQDGDTDLFIGGRVAPYYGFNTDSYFLINDGKGKFSVLE
                     DPVTRGLGMVTDAVWADVDPTPGKELVVCGEWMPLTILSREGNRWKKRMIPSSSGLWQ
                     TVKAADLDGDGDQDLVAGNLGMNHDLKASPQHPVMIYPADFDQNASPEAPVSYYREGK
                     DYSFFSKDELAGQLVFLKKKYTDYRSFSKQDFDQIFPKSRDDQNVRKVEITASAMFEN
                     KGDGHFTIIELPLEAQFSMVLALCLDEVSGDSLPDILLGGNLYETLPAFGRQDAGHGL
                     LLLNQGKMAFRALPAEQSGVFIDGAVRDIEILHTGSSKKYLFVRNGATPVVLHR"
     gene            complement(7625..10933)
                     /locus_tag="PKM27_13600"
     CDS             complement(7625..10933)
                     /locus_tag="PKM27_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNR08347.1"
                     /translation="MNKSSKIHVLILLAGCCWSCVQKDKPGVVPPLFTLMPASHTQVE
                     FSNPLHYDREFNIYRYRNFYNGGGVAIGDINRDSLPDLYFTANMAPNKLYLNKGNFVF
                     EDITESAGVGGTKAWSTGVAMADVNGDGWLDIYVCNSGDIKGDNKENELFINQGDNTF
                     IEMAEAYGLNDRGYSTHAAFFDYDKDGDLDMYLLNNSYQAIGSFNLRNNIREERDTLG
                     GDKLFRNDGKKFTDVSDEAGIYGSLIGFGLGVTVGDINQDGWQDIYVSNDFFERDYLY
                     INQKNGTFTEELTRQMPSISAASMGADMADINGDAYPEIFVTDMLPSTEKRLKTKTTF
                     EDWNKYQYNVKNGYYHQFIRNMLHLNHGDGSFSDIGRLAGVHATDWSWGALIFDMDLD
                     GRKDIFVANGIYQDLTDQDYLSFVSDENTVRNIVKDDRVDYKQLIDSIPSEAIPNHAF
                     HQGEGLQFSDSAAAWGLATPSFSNGAAYGDLDLDGDPDLVVNNVNMPCFIYRNESTSR
                     LGHHYLKIILKGKGGNTYAFGSQIKVYAGAKLQYLEYMPIRGFESSMEPVALFGLGKA
                     ESVDSLVIVFPDQSIIRISPVKADQTLFLDQREAIPAQTFAAKPASKSLFEPLTVKGV
                     SFMHSENDYSDFDRERLVYHMTSSEGPKVSIGDLNGDQLQDFFIGNAINAFGAIYLQQ
                     RDGSFSHTNQPAMEGHRASEDTGSGLFDADGDGDLDLYVCSGGSEYSEASSDLMDRLY
                     LNDGKGQFTWTRQPLPTFRFESSSCVRPCDVDGDGDEDLFVGIRAKPFNYGLPVNGYL
                     LINDGKGSYTDETKTRAPRLLQLGMITDARWADLDGDSVKELIVCGEYMPLQIFKNTG
                     GQWALVQAAGPTGWWRCLLDVDIDGDGDMDLVAGNHGLNSRFKTQPGHPLKMYVNDFD
                     QNGTVEQIITTFREGRDYPFTLKHDLLAQMPVLKKKYLHYSDFAGKTLDDFFSPAQRA
                     NTWVDSVTTLETTLFINDGKGRFSSKALPVEAQFSPVYALFSDDFNGDGKKDLILGGN
                     LYRTKPETGRYDASYGLVLTQTENQEFRALPATESGICIRGEIRDIQAIRIQGKKALI
                     IARNHDRPLFYTYR"
     gene            complement(10926..11507)
                     /locus_tag="PKM27_13605"
     CDS             complement(10926..11507)
                     /locus_tag="PKM27_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08348.1"
                     /translation="MYIVTRIHFFLLACLVLAVSCKSRYERMLERELASGERHDSLFL
                     GLTLGMDRKTFFEICWNYNKKGEMTQGSNNLSARYFLNEGLKAPAYLDFYPTFSNDSI
                     VEMPALFTYKDWAPWIKAYSTDSLLVDVKQLLERWYGPGFIEIKNKQKGNLWVKVDGN
                     RRIRFFKREPREVRMIITDMSKTPQTVNGAGNE"
     gene            complement(11559..14858)
                     /locus_tag="PKM27_13610"
     CDS             complement(11559..14858)
                     /locus_tag="PKM27_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNR08349.1"
                     /translation="MPRYLLLISILFCACKPNNTTVDEPATPLFRLLSSLRTGINFAN
                     NLTYTEEFNPYTYRNFYNGGGVGLGDINNDGLLDIYFCGNQVDNKLYLNKGNLAFEDI
                     TAKAGVACPGVWSSGVAIVDVNGDGWQDIYVCKSGKPGGDRRHNELFINQGDLTFREE
                     SAQYGLNTAGLSSHAAFFDYDRDGDLDVYLLNNSIRTVGVYDLRKDQRLIPDTLGGNK
                     LMRNEGGHFTEVSSQAGIYTSAIGFGLGVSVGDLNRDSWPDLYVSNDFFEKDYLYLNQ
                     RDGTFKECLDSAMSEISLNSMGADMADIDNDGLPEVFVTDMLPETDARYKTKTTFENW
                     EKYQRNVQNGYHHQFIRNALQWNRGGLQFSEISRYAGVYATDWSWGALITDLDLDGHK
                     DIFVANGIYKDLTDQDYLNLYNDPETILGLMNKENQVIKKLIDAIPSEKIPNCVFRNQ
                     GDLTFTNLAAEWGLGEASFSNGSSYGDLDNDGDPDLVVNNVNMPCFIYENTSDKNPNR
                     HYLRFEPEGSGANTLAIGTQITLYSGDRKWFQEVHPMRGFMSCVDPRPLFGLGSAEAV
                     DSIRIIWPDGRTMSLGHTPADQSIKLRPSPGLPFDSIPLQSEKTVLWERVYPGGLDFI
                     HQENAFNDFERDRLLFHMISREGPALATGDVNGDRLEDLFIGNAMDAEPALFLQQKSG
                     YFKSLKSGPLAQDKGSEDTDATFFDADGDGDLDLYVCSGGNEVPAGSSLLADRLYFND
                     GRGHFKKSNQILPTFNFESSSCVRPCDFDRDGDLDLFVGIRMVPFNYGVPCNGYLLEN
                     DGKGQFNPREVPALQKLGMITDAAWADFDGDGDHDLAIIGEWMPVTLLKNENNQFTDA
                     TAAYALDQSTGLWQSIRAGDLDGDGDQDLVAGNWGLNSRIKSTDKHPARLYINDFDQN
                     GSAEQILTVFNGDDYPLTLRQDLVMQIPSLKKKYLRFESYKNQKITDVFTPQQLSGAI
                     HLQCHTSRSGLFINQGDRFEFKSLPPEAQLSPVFSVLIEDLDRDGLMDLLLGGNYGSA
                     KPETGIHRAQGLSIFLQNKNHDFIYNASGHALHQEVRNIQALDIGQSRHFVLAVNNGI
                     PVVLKRQMEDK"
     gene            complement(15327..16358)
                     /locus_tag="PKM27_13615"
     CDS             complement(15327..16358)
                     /locus_tag="PKM27_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR08350.1"
                     /translation="MKYLVFLLALLTGPASSAQMPAGKVVSFVLLSKALQNPGGENPN
                     RKVSVYLPPDYEQAGKRFPVIYYLHGFMGTDSIYPGMKMILDLAIHRKKIQPFILVQA
                     SQYTLYEGSFYTNSSLTGNWADFTAKELVEYVDLNFKTIANRDGRGIAGHSMGGYGAL
                     KLAMLHPEVFCSTYALSPGLLALVKEFGPNSESFKELARIKTHEELKRSYYPRVLVAV
                     ARAWSPNPDKAPFYCDVPFTYQGDSLLVDQTVLEKWHQNMPVYMVDRYAGNLRKLKAI
                     KLDWGRNDSSRFPVQCGMLSQRLENLGIEHFAEEYIGNHVNKIWTADGRILNEVLPFF
                     NDYLVFETK"
     gene            complement(16394..17929)
                     /locus_tag="PKM27_13620"
     CDS             complement(16394..17929)
                     /locus_tag="PKM27_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNR08351.1"
                     /translation="MKIFLLYPIVFTLFAGARQNETALTLVTTKSGKLSGVLNEDKSI
                     SGFKGIPYAAPPVGELRWKEPQQVKPWTGTKVCDKFPASPYQNKPAPFAMWTEEFIAP
                     PEPLSEDCLYLNVWTPAKSAKEKLPVVMWIYGGGFVSGSSACAIYDGEALARQGIVFV
                     SINYRVGVFGFFSHPDLTKESGKNASGNYALLDQLAALRWIKDNITEFGGDPDRVTIA
                     GQSAGSFSVQALVASPLSKGLFRGAIAHSGASMGRFSKNLIDAEKSGLELAYKIQAES
                     VVALRNLSADSLLKLANTLPFGTFAPVIDGYVIPEDLGSIFLNKKHQDVSLMAGWVTG
                     DAALSGPVKTAEQFKTWVSSTYPSHKDEFLNAFPSATDEEARQSQLKLGNLNFAGYAD
                     HQWALNNDSNSYLYQFSHVPTDKPGFPNYGAFHTSEVPFALHTLSKWNRPWKDSDYGV
                     EKYMSAYWSNFVKTGNPNGAGLPEWKNYEAASQNIMELGEKPVLKPGMFKAELRVLER
                     IKK"
     gene            complement(18041..19144)
                     /locus_tag="PKM27_13625"
     CDS             complement(18041..19144)
                     /locus_tag="PKM27_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR08352.1"
                     /translation="MAQKVSNTPIEDSGEAEPTVSEKQHIEILIEEQIKRFQVICSSE
                     NDAIITSDESKRILFWNKGAEIIFRYTSEEALGKPLTLIIPGSLHHRHEEGMERMNQR
                     EKPRVLGSVLELKALRKGGEEFPIELTLGSWDHGGKRYYSGIIRDITEKKRAEATILD
                     EKKKADTLLLNILPRQVADELKNKGKAAAKKYDQVSILFTDFKDFTSLASSITPIKLV
                     KELNDIFGHFDEILEVFKIEKIKTIGDAYFAACGLPEKNRDHAILCIEAAKQMFRYLE
                     ARNKKSSIQWKMRAGIHSGPVVAGVLGKKKFAYDLLGDTVNTASRMESSGEVGKINIS
                     ETTYALVREKYKCVPRGQINAKGKGELNMFFVE"
     gene            complement(19408..20949)
                     /locus_tag="PKM27_13630"
     CDS             complement(19408..20949)
                     /locus_tag="PKM27_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR08353.1"
                     /translation="MKKFHENLVGKSLALIALLLICCKTLWAQPDLNKLDAYYQKALQ
                     DWNVPGMSIGIVKDGKLVFAKGYGTMEAGKNQTPDENTLYAIASNSKAFTSAAIAMLV
                     QEGKIAWDDKVKKYLPYFELYDRYVSDETTVRDLLCHRVGLGTFSGDVIWYRSDLTAE
                     EIIRRLKYLPKAYDFRSGYGYSNVMYVTAGEIIKTVTGKTWGEYITEKILKPLGMSRT
                     VYATRFLPGLSNVATPHEFLDGKNKPIPWVDWETVAATGGLISSVQDLAQWLILNMNH
                     GIRGKDTLLTKTSRNTLWTPHNNFPVDHTEKNALTHFRGYALGWGISDYQGKMRIGHT
                     GGYDGMISTIQMLPDENLGFIMLTNGLQAPIGALPNYTFDAFLGKDSRDWSKEQLENL
                     ARNKKSDTRIEDRKKARVMGTQSTLPADRYAGTYYANIYGNIKVTHHAGQLKIDFEHT
                     PDFSATLRHWHYDVYELVWDKPQAWFQLGTVRFVLDNNLKVTGLEFDVPNDDIFFEEL
                     KAVRK"
BASE COUNT         4750 a         5366 c         5723 g         5483 t
ORIGIN      
        1 ggcttatatt tttttttatt acctcgtctg ggagttttat ttcagggggt tcctattgtt
       61 tggcctggcc aagatttacg gaccggctgc tgccattctt atacaaacca tttcttcctg
      121 cctggtgcac ctgggcaagc cggaaggaga gacgctgggc tcggtggccg ccggcctttt
      181 atttggcctg ctcgctctcc ggaccggttc gatctggtat ggatggctga tccatttttc
      241 actcggggtg atgactgatt tttttattct ccgcaacgcg ggggtttcgc tgttttgaaa
      301 aagggagatc gatgaatatc ctgatcaccg gggcaaatgg ttttataggc agccgcctgg
      361 ccatgcgcct tactgaaatg ggccataaga taagggggct ggtaagggaa acttccaggc
      421 tggacaagct gaatgggctg gaactggaac tagcctttgg agacatcacg gtccctgaaa
      481 ccctcgtcaa acccatgcag ggtatggacc tggtcattca caccgcaggc gccgtcaccg
      541 actggggtcc gctttcctat tttgagcagc aaaatgtgga tgggcttcag caggtgttca
      601 aagccgcgga ggccgccgga gtcgggcgct ttgtgcatat cggtacggtg gctatttatg
      661 gttttggcag gataggggtg aaagaagacg atcctttcgc ggaaagccat gtgtgctatt
      721 gtatttctaa acggaaaggg gtcgaatggt tgaaacggtt ggccggacac actaaaatgg
      781 aaatgaccat cgtccatccg ggaaatgtgt ttggaccggg ggatgacaaa tttatcctgc
      841 cgtttctgca attgttggac cgcaaccgtt tcgtatgggt caatggaggc agggcctgga
      901 cctgtccgac ttatatcgaa aacctggtcc aggggatcgc tcttgcctct tttcatcccg
      961 ccgcggcggg ggagactttc atcattacag acggcttaga gatggattgg aaaacgtttg
     1021 ccggggcctt gtttcgcggg ctgggcaaaa agcctgtaac cctttccgta ccgcgcccca
     1081 tcgtctattc cgttgccttc atgctggaaa ccgtttacaa acttatccat tcccggcaag
     1141 ccccggcact cacccggtac cgcatcgaca atgcaggatt ggattatcat ttttccatcg
     1201 aaaaagcgca aagactcctt ggctatttac cgccggtgga tttccaggcc agcattgacc
     1261 ggaccgtgca gtggtatggt gagaggaaat taataaaatt aaaaaattaa gaattaagaa
     1321 ttaaggattg ggccggtcca ggatccatac tccccggcca taggtggcga tcacgatttg
     1381 tttggtagtg gggtggataa acaggtcctg taccgagacg gaagccggga ggttgccgtg
     1441 catggcattc caggtcttcg ccccatcggt gctccggtag atgcccatat cggtccctgc
     1501 gtataaaata tccttgttgt ccgggtcctc gcgaatgatg ttgacgggtg aagcgggcag
     1561 gttggagttg attttaatcc agttacggcc ataatccgat gatttgaaaa gcaaaggcgt
     1621 gatgttgtct tcccgccggt cagactggct gagatagacg gtcgcatctt caaaacggga
     1681 gggttctata ctgctcaccg cggcatcttc cagcagcccc ttgctgattt ccgtccattt
     1741 gccgttgcct tccagggtgg tgaaatgggc tttgccgtca tccgtccccg cgtacaacaa
     1801 tccctttttc tttggggatt cggccagggc tgaaatggtt tggtaattga tccggtaggg
     1861 cagagtgccg attttatttc tgttgttttg ggtcaggtcg gggctgattt tttcccaggt
     1921 cttgccttta tcggtggtct tcaatacgta ttgcaggccg tgataaaggg tggtgccgtc
     1981 gtgcggagaa atgatcgtgg cggcaagcca ctcgccccgg tgttcttctc ccagcaggct
     2041 tacgggcggg tcaatggatt cgctggctcc ttttttaccc agccttttca ggttgccctt
     2101 catgatatgt ccgtaatacg aagaagaata gaggatatcg ggttctttct ggtcgatggc
     2161 aatgcgggtg ccttcccctc ctggaatcca gtcgaattta cccgtagacc ggtcggccgg
     2221 gtcataggtc gtggtgctca gcatgcgcac gcttccttcg tcctggatgg agccgtagat
     2281 ataaaaggga gtcctgggat cataggtgac gttgtaaaat tgggtggtgg ggagttcatg
     2341 gtaaaagcct ttccaggtca gcccgccgtc atagctgacg ttcactccgc cgtcattggc
     2401 attgatcaaa taattgggat cggtgggatc gatccaaaga ccgtgatgat cgccgtgtac
     2461 ccggccccat tcccccttgg gccctttggt gagggatttc caggttttgc cgccatccgc
     2521 actcatcgtc aggtccaggc ccatcaggta caccttgttt tcatcggttg gggacacccg
     2581 gacctgggca aaaacccatc cataggttcc tgaaaaaaat tccataaaat cgtcgtgctc
     2641 gctcatcctt ttccaggact ctcctttatc cgtgctttta tagacttcag ctcccttgat
     2701 cacaactact ttaggccggt tataggaatc ctgctccccg gggttggggt cccttttctt
     2761 gccgtggttg tccacaaaag cgtagagtac attcgggttt gccggagtgt tggccaggcc
     2821 gatccgcccg gtctctttgg tatccggcag cccattattg atgattttcc aggtttcgcc
     2881 cgcatcggtg gacttgtata tgtggtcgcc gtcctccggt acgggatcac tccagcgttt
     2941 gcgtatccgg gtccacatgc tggcatagag ggtattcggg tcagaagggt ccatgaggag
     3001 atcgttggcc cccgttttgt cattcacata aagtattttt ttccagttaa gcccgccgtc
     3061 cgtggattta tagaccccgc ggtcttcgtt gcggctccat tcattgcccc ccgcggctac
     3121 ataaacaata ttggggtcgg tgggatgaat caggatccgg gaaatggtgt ttgtgttttc
     3181 aaggccaaga tggttccaga aatttcctcc gtcggtgctt ttgtacacgc ccgtaccggc
     3241 taaagaagcc ctgaaaatat tagcttcccc cgttcccaca tagatcacgt ttggattgga
     3301 gggcgcgatg gccatattgc cgatggcctg ggtggcctgc ctgtccatca atggggtcca
     3361 ggtcttgccg gaatccacgg ttttccataa cccccctgtg gcaaagccgg catacatgat
     3421 ctttttattt cccgggatgc cccaggcatc cgtgctcctg ccgctgatat tggccggatt
     3481 caacaatttc cattttactt cctggtaagg gctggaagcc aaaagccttt catgctggat
     3541 gaaagcaatt cttttttccc tgccggtact ggggcgctgg ttggtttccg gttgggaaaa
     3601 aacggcgctc agggccggga ggatcaggat ggcgatcagc agaattcgtt tcatgattgg
     3661 cggattaaaa agcaggccaa aaataacaga atttaacggc tgcggtgacc gttgagcacc
     3721 cgcagtcaac gataaaacaa gacggacaag gcaaacattg cctttgtggt tcaatgacca
     3781 aataagttga caaaaaactg gtcaagcccg aataaaacgg cgacggtgag cagggcggcg
     3841 aggatttgca accaggccat tcgggttccg ggagacagcg tatgatccag ggacatcaaa
     3901 ccggaaaggg ccttgaggat ctttttccgg tttagaaaca ggatgatcaa aaccagggaa
     3961 aaataaagca aggcgctggc ggtcgcccca tgtttgtcca tgaacaggag gtcaaagacc
     4021 acgacattgg ccagaatagg cagcaggata aaggcgccga tcagtttggt tctttcccag
     4081 atcaaaagcc aggcgccggc gacctgcaaa atgccgacaa agaggatata attaaacgaa
     4141 tatcccataa aggtccaggc caggtcaaat gcattggctt ctccaagggt ttggcccgca
     4201 acttcagctg gcagatgacc tctgcggtaa aactggccgc ccagcatttt gccccatccg
     4261 taaacattca gcattataaa aacataccat cgcgcggctt tttcaagtac gatcctggaa
     4321 ttgagacttt gcaaaaggga catgtaaacc tgcgggagcg gttttcaacg gtgcaataca
     4381 accggtgtgg ctccattccg gacaaataaa tactttttcg aactgccggt atgtaaaatt
     4441 tcgatatccc ttaccgcacc atcgatgaag actccgcttt gctctgccgg caaggccctg
     4501 aaggccatct tgccctggtt tagaagcagg aggccgtggc cggcgtcctg gcggccgaaa
     4561 gcgggcaagg tctcatagag atttccgccg agcaggatat ccggcagcga atccccgctg
     4621 acctcatcca ggcataaggc gaggaccatg gaaaactggg cttccagcgg caattcgatt
     4681 attgtaaaat ggccatcgcc tttattttca aacattgcgc tggccgtgat ttcaaccttt
     4741 ctgacatttt gatcatcccg gctcttcggg aatatctggt caaagtcctg ctttgaaaaa
     4801 ctgcggtagt cggtgtattt tttcttcaga aagaccagct ggccggccag ttcatccttt
     4861 gaaaaaaaag aatagtcctt accctcccgg tagtaggaga ccggcgcttc cgggcttgcg
     4921 ttttgatcaa agtcggcggg atagatcatg accggatgct gtggagaggc tttcagatcg
     4981 tggttcatgc ccagattacc cgctaccagg tcctggtccc catctccatc gagatccgcc
     5041 gctttgaccg tctgccatag cccgctgctt gatggaatca ttcgtttttt ccaacggttc
     5101 ccttctctcg aaaggatggt taggggcatc cattccccgc aaaccaccaa ttctttaccc
     5161 ggggtgggat ccacatccgc ccagacggca tccgtcacca tgccgaggcc cctggtcacc
     5221 ggatcttcca aaacgctgaa tttccctttg ccgtcattga tcagaaaata ggaatcggta
     5281 ttgaatccgt aatacggggc cacccttccc ccgataaaca ggtcggtatc tccgtcctgg
     5341 tccaggtcat gtgccgcaat acatgcgctc atatgggcct gggccggaaa accatcctcc
     5401 cggaaagtca gattgccctt gccgtcattc aggtaaaggc ggtcaaccaa aagggcgggc
     5461 tggtccatct ggtaacctgc cgaggccacc gccagatcag gatccccatc ctggtccgcg
     5521 tcgatccagg atccgtccac atcttcaaaa gaggcgtgca ggtcgaggct gttctgagcc
     5581 agggccgtaa atgatccgga cggtgtttgc agataagtaa tgcccgcctg acccctggcg
     5641 ccgccaatcc aaaggtcttc cagtccgtcg ccgttgatat ccgcgaccgc gctgccgggc
     5701 ccctgggtgg acaatttata gagcagcaat ttttcccttt caaaatcatt gtagggattt
     5761 tcaacatgta gataagcgat accggggtca gctgctttaa acaaggtttc ccttgaaacg
     5821 ggtgcagcat acccctcctt ccggtcttgc tgctttatgg tatttaagcg gtccggctga
     5881 accgcttcga tgcgctgcag ggagccatcc ggccatcgga tcaggaggct gtcgatgccg
     5941 gtatggctgc ccaatccgaa gtgcaccgta taatccgaag aagactgaaa gccccggaca
     6001 ggaaagattt ccctggtttt tttttggtcc ccctggaaaa gggttaccct cgcgcccact
     6061 gcaaatggat ttagtccctg gccttccagt tttatcctga gggaatggcc cggcggctgg
     6121 gtttcattcc tcagcaggct ggccggctca ttgatccggt tgatgaccag gtcgagatcc
     6181 ccgtctaagt caaagtcagc ataagcagcc ccggttgaca agtccgggcc ttccgggttc
     6241 caaagggcag atcggtcggt gaaaagcaag gggctttcct ggctgaagaa ataattcctg
     6301 acccggccat ccggcatttt ttggatcagg gaccctggat ccgaagtatc ggaggcggag
     6361 ggagccgcca ggtatttcag gtaatcgagg tcgttcgggc ggtgggctat gccgttggct
     6421 acaaagaggt ccaaactacc gctgttgtca aaatcggcca gcagcggagt ccagctccaa
     6481 tccgtcctgg agatattgct caactggccg atttcactga aaatcgggag gccatccgga
     6541 cggatgaagg tattcaactg cagggtgttt ctgggatact gaaagtggta cccgaagtca
     6601 gccttatatt gatagatatc gtaaggatcg gctcctaccg aatttttcag cacttcttcc
     6661 gattcaggct tcatgtccag gctcacgata tcggtgaaac cgtcgtcgtt gatgtcggcg
     6721 atatcgctgc ccatggaaaa agtgctggtg tggccgaatg aaagggtctg gctttgccgg
     6781 aaggtcccgt cctgccggtt aatatagagg taatcgtttt catggaaatc gttgccgatc
     6841 aggatatcgg gccagccatc cgcgttaaaa tccgaaacgc ccacccccag cccgtaaccc
     6901 agggggctgc tgaggatgcc ggcttccgga ctgacgtccg taaatttcat tccatcgttt
     6961 ctcatcaacc ggtccccttt ctgggggtct gcctggcggc ggatcgaggt atcccggtag
     7021 gtttccacgg aatggatgga atggcagagc agatacatgt ccaggtcgcc gtcacggtca
     7081 taatccagcc aggctgcctg ggtcgaaaaa gctttaaagt caaggccgta ttgatttgaa
     7141 agttccgtaa aggtcaggtc ccggttattg atataaagcc ggttttgtcc attaaaactt
     7201 ttataatccc cgatcgcgca aacataaata tccagccatc cgtcgccgtt gacatcggcc
     7261 atgctgaccc ccgtattcca ttggttttca caggcggtcc cggaagcctc agtgatatct
     7321 tcaaaccgga aatttccctt attcaaatac agtttgttgc ctgtttcatt ggaggcaagg
     7381 tagaggtcgg gcagcccgtc gttgttgagg tcacccgcgg ctacgccggc tccgttgtag
     7441 taatacaggt attcgaggat attgaccggg cctttttcat tcagcaggtt cgaaaagcgg
     7501 ataccgcttg aatcggccgg gacggaagta tagagggtag cctgtttttt ttctttgcag
     7561 gagggccaca atccaatcag gacggccagg acaaacaggg tttttatttt accgtccata
     7621 ggcctcagcg gtaggtataa aacaaaggac ggtcatggtt gcgggcgatg atcagcgctt
     7681 ttttaccctg tatccggatg gcttgaatat cccggatctc gccgcgaatg cagatgccgg
     7741 attcggtagc cggcaaagcc ctgaactcct ggttctccgt ttgggtaagc accaggccat
     7801 aggaggcatc gtagcgcccc gtttccggtt tggtgcggta taaattgcct ccgaggatca
     7861 ggtctttttt tccatccccg ttgaaatcat cggaaaaaag ggcataaacc ggtgagaact
     7921 gggcttccac cggaagggct ttggaactga acctcccttt gccatcattg ataaacaggg
     7981 tggtttccag ggtggtcacg ctgtccaccc aggtattggc cctttgggcc gggctgaaaa
     8041 aatcatccag ggtcttaccc gcgaaatcag aataatgcag atattttttc ttcaatacgg
     8101 gcatctgggc cagaaggtcg tgtttcagcg taaagggata gtcccgccct tcccggaagg
     8161 tggtgatgat ctgttccacc gtgccgtttt ggtcaaaatc attgacgtac atctttaaag
     8221 ggtgaccggg ctgggtttta aaccggctat tgaggccgtg gttgccggct acgagatcca
     8281 tatccccgtc tccgtcaata tcgacgtcca acaagcagcg ccaccagccc gtgggtcctg
     8341 cagcctggac cagcgcccat tgcccccccg tgtttttaaa gatttgcagg ggcatgtatt
     8401 cgccgcaaac gataagttcc tttacagagt ctccgtcaag atcggcccac ctggcatccg
     8461 tgatcatgcc aagctggagc aggcggggag ccctggtttt ggtttcatcc gtatacgatc
     8521 cctttccatc gttgatcaga aggtaaccgt tgaccggaag tccgtaatta aaaggtttgg
     8581 cgcggatccc cacaaagagg tcctcatccc cgtccccatc gacatcacag ggacgtacgc
     8641 aagagctgct ttcaaagcgg aaggtgggca ggggttgccg ggtccacgta aactggcctt
     8701 tcccgtcatt gagatacaag cggtccatca ggtcggagga agcttcggaa tattcgcttc
     8761 caccgctgca tacatagagg tcaagatcgc cgtctccatc ggcatcgaat aagccactgc
     8821 cggtgtcctc gctggcccgg tggccttcca tggccggttg gttggtgtgg ctgaaactgc
     8881 cgtcccgttg ctggaggtag atggcgccga aagcattgat ggcattgccg ataaaaaaat
     8941 cctgcaattg atccccattc aggtccccta tggatacctt gggcccttcc gaggaggtca
     9001 tgtgatagac cagccgttcc cggtcaaaat cggaataatc attttctgaa tgcataaacg
     9061 aaacgccttt gaccgtcagc ggttcaaaca gactttttga agccggttta gccgcaaagg
     9121 tttgggcggg tatggcttcc cgctggtcca gaaacaaggt ttgatcagcc tttaccgggc
     9181 ttatacggat gatgctttga tccggaaaaa caatgaccag ggaatccacc gattcggctt
     9241 tccccagtcc aaacagggcg accggttcca tgctcgattc gaaaccgcgg atgggcatgt
     9301 actcaaggta ttgaagcttc gctcccgcat aaactttgat ctgggaacca aaagcgtagg
     9361 tattgccccc tttgcctttc aaaatgatct taaggtaatg atggcccagg cggctggtcg
     9421 attcgttgcg gtaaataaaa cagggcatgt tcacattatt gaccacgagg tcaggatcgc
     9481 cgtccaggtc gagatcgcca taggcggcgc cattggaaaa agaaggggtg gccaggcccc
     9541 aggccgcggc ggaatcgcta aactgcaatc cttcaccctg gtggaaggca tggttgggga
     9601 tggcttcact gggtatggaa tcgatcagtt gtttataatc gacccggtca tccttgacga
     9661 tgtttcgcac cgtattctca tcggagacaa aactcaggta atcctggtcg gtgagatcct
     9721 ggtaaatgcc gtttgccaca aagatgtcct tgcggccgtc caggtccatg tcgaaaataa
     9781 gcgctcccca gctccagtcc gtggcatgta caccggccag gcggccgatg tccgaaaagc
     9841 tgccatcgcc atgattgagg tgcagcatat tgcggataaa ctggtgataa tacccgtttt
     9901 taacgttgta ctggtatttg ttccagtctt caaaggtggt tttggttttc aggcgcttct
     9961 cggtggacgg cagcatgtcg gtgacaaaga tctcggggta agcgtcgcca ttgatatcgg
    10021 ccatgtccgc ccccatggag gctgcgctga tgctgggcat ttgccgggtg agttcttcgg
    10081 tgaaggtgcc gtttttctgg ttgatataga ggtagtcgcg ttcaaaaaaa tcattgctga
    10141 catagatgtc ctgccagccg tcctggttga tgtcgcccac ggtaaccccc agcccgaaac
    10201 cgatgaggct gccgtatatg cccgcctcgt cgctgacatc ggtaaatttt ttcccgtcat
    10261 tccggaaaag tttgtccccg cccagggtat cccgttcttc acggatattg ttgcgcaaat
    10321 taaagctgcc gatggcctgg taggaattgt tgagcagata catatccagg tcgccgtcct
    10381 tgtcgtaatc aaaaaaagca gcgtgggtgg aatatccacg gtcgttgagg ccgtaggctt
    10441 cggccatttc aatgaaggta ttgtcgccct gattgatgaa aagttcgttt tccttgttgt
    10501 cccccttgat atcccccgaa ttgcagacat agatgtccag ccagccgtcc ccgttgacgt
    10561 cggccatggc caccccggtg gaccaggcct tggtgccgcc taccccggcg gattcggtaa
    10621 tgtcttcgaa tacaaagttt cctttgttga gataaagttt attaggagcc atattggcag
    10681 tgaaatagag gtcaggcagg ctgtcgcggt tgatgtcccc gatggctact ccacccccat
    10741 tgtaaaaatt ccggtagcgg taaatattaa actcgcggtc gtaatgcagc gggttggaaa
    10801 actcaacctg ggtatgggaa gccggcatca gggtgaagag gggagggacg acacccggct
    10861 tatccttttg tacgcaagac cagcaacatc cggcaagcag gatcagaaca tggatttttg
    10921 aagatttatt catttccggc cccgtttaca gtttgaggag ttttggacat gtccgtgatg
    10981 atcatacgaa cctcacgggg ttccctttta aaaaaccgga ttctgcggtt gccgtccact
    11041 ttcacccaca aattaccttt ttgtttgttt ttgatctcta taaatcccgg tccgtaccag
    11101 cgctccagca actgtttcac atccaccagc agactgtccg tggaataggc tttgatccag
    11161 ggtgcccagt ctttataagt gaagagggcg ggcatttcca caatggaatc gttgctgaag
    11221 gtggggtaaa aatccaggta agcgggggct ttgaggcctt cgttcagaaa ataccgcgcg
    11281 ctcaggttgt ttgagccctg ggtcatttcg ccttttttgt tgtaattcca gcaaatctca
    11341 aaaaacgtct tccggtccat gcctaaggtc agtccaagga acaaactgtc gtgcctttcc
    11401 ccgctcgcca gctcccgttc gagcatacgc tcataccggg acttgcagga gacagccaga
    11461 accaggcagg ccaggaggaa gaagtgaatg cgggtcacga tgtacaaagc ctcaaagata
    11521 gaattataga aattaagaat taaaggaatg aagggggact acttgtcctc catctgtctt
    11581 tttaagacca ccggaatacc attattcacc gcaagtacaa aatgcctgga ctggccgata
    11641 tccagggcct ggatattgcg gacctcctgg tggagtgcat ggccggaagc attgtaaata
    11701 aaatcatggt ttttgttttg aaggaagatc gaaaggccct gggccctgtg gatcccggtt
    11761 tccggttttg cactgccgta attgccgccc agcaggaggt ccataagccc gtcccggtcc
    11821 aggtcttcga taagcactga aaataccggg gatagctggg cttcgggggg gagggatttg
    11881 aattcaaacc ggtcaccctg gtttatgaac aaaccactgc ggctggtatg gcattgcagg
    11941 tggatcgcgc cgctgagttg ttggggggtg aatacatcgg tgatcttttg attcttgtag
    12001 ctttcgaaac gaaggtattt tttcttcagg gaagggatct gcatcacgag gtcctggcgc
    12061 agggtcaggg ggtaatcgtc gccgttgaaa acggtgagga tctgttctgc ggatccgttt
    12121 tggtcaaaat cgttgatgta gagacgggcc gggtgcttat ccgtggattt gatcctggaa
    12181 ttcaggcccc agttacccgc caccaggtcc tgatcgccat ccccgtcgag gtccccggcc
    12241 cggatggatt gccataggcc ggtactttgg tccagtgcat aagcagcagt ggcatccgtg
    12301 aactgattgt tttcattttt caggagggtg accggcatcc attctccgat tatggccagg
    12361 tcgtggtccc catcgccatc gaagtcagcc caggccgcat cggtgatcat ccctaatttc
    12421 tgaagcgcgg ggacttccct gggattgaac tgacccttgc cgtcgttttc aaggaggtaa
    12481 ccgttgcagg gcacgccata gttgaagggg accatgcgga tgccgacaaa aagatccagg
    12541 tccccgtccc ggtcaaaatc gcagggccgg acacaactgc tgctttcaaa attaaaagtc
    12601 ggaaggatct ggtttgattt tttgaaatgg ccccggccgt cgttaaaata aaggcggtcc
    12661 gccaggagtg aggacccggc cggcacttca ttgcccccgg aacacacata gagatccaaa
    12721 tccccatccc catccgcgtc gaagaaggtc gcgtcggtat cttccgatcc cttgtcctgg
    12781 gccaacggtc cggacttcaa tgatttaaag tacccgcttt tttgctggag gaacaaggcc
    12841 ggctccgcgt ccatcgcgtt tccgataaaa agatcttcca aacggtctcc gttgacatct
    12901 ccggtcgcca gggccggccc ctccctggat atcatgtgga agagcaggcg gtccctttcg
    12961 aaatcattaa aggcattttc ctgatgaata aaatcaaggc ctccgggata aaccctttcc
    13021 cataaaacag ttttttctga ctgcaagggg atgctatcga acgggagacc gggggatggc
    13081 ctgagcttta tggactgatc tgccggggta tggccgaggg acatcgtccg gccatccggc
    13141 cagatgatcc ggatggaatc gaccgcttcc gcggaaccca ggccaaacag gggccttgga
    13201 tccacacagg acataaatcc gcgcatggga tgcacctcct ggaaccattt ccggtccccg
    13261 gagtataagg tgatctgggt gccgatggcc agggtattgg cgccgctccc ttccggttca
    13321 aaacggaggt aatgccggtt ggggttttta tctgatgtgt tttcatagat aaagcagggc
    13381 atgttgacat tattgaccac caggtcgggg tcgccgtcat tatccaggtc cccgtaagag
    13441 gaaccgttgg aaaaagaagc ttcgcccagg ccccattccg cggccaggtt ggtgaaggtc
    13501 aggtcgccct ggttgcggaa gacacaattg ggtatttttt cggaaggaat ggcatcgatc
    13561 agctttttga tgacctggtt ttctttattc atcaggccca ggatggtctc gggatcattg
    13621 tacaggttga ggtagtcctg gtcggtcagg tctttataga tcccgttggc gacgaaaata
    13681 tccttatgtc cgtccagatc caggtcggtg atcagggcgc cccagctcca gtcggtggca
    13741 tagacgccgg cataccggct gatttcactg aattgaagac ctccccggtt ccattgcagg
    13801 gcattccgaa taaactggtg atgatagccg ttttgcacat tgcgctggta tttttcccag
    13861 ttttcaaaag tggtttttgt tttatagcgc gcatcggttt cgggcaacat gtccgtgacg
    13921 aagacctccg gcagtccgtc gttgtcgata tccgccatat ccgcccccat gctgttgaga
    13981 ctgatttcgg acatggccga gtccaggcat tctttgaagg tgccgtccct ctggttgaga
    14041 tagaggtagt ccttttcaaa aaaatcattg ctcacataga ggtccggcca actgtcccgg
    14101 ttgaggtcgc cgacgctgac ccccaggcca aaaccgatgg cgctggtata gatgcccgcc
    14161 tggctgctga cctcggtaaa atgcccgccc tcattccgca tcagtttatt acctccaagg
    14221 gtatcgggga tcaagcgttg gtccttacga aggtcataga cgcccaccgt gcggatcgaa
    14281 ttattcagca ggtagacatc caggtcgcca tcccggtcgt aatcaaaaaa ggcggcatgg
    14341 ctggaaaggc cggcggtatt gagcccatat tgggcggatt cctcccggaa ggtcaggtcg
    14401 ccctggttga taaaaagttc gttgtgtcgg cgatcgcctc cgggttttcc ggatttgcag
    14461 acatagatgt cctgccagcc gtcgccgttg acgtccacga tggccacccc gctggaccag
    14521 accccggggc aagccactcc ggctttggcg gtgatgtcct cgaaggcgag atttcccttg
    14581 ttgaggtaaa gtttattatc cacctggttg ccgcagaaat agatatccag gaggccgtcg
    14641 ttgttgatat cacccaggcc gacgccgccg ccgttgtaaa aattccggta ggtataggga
    14701 ttaaattctt cagtataggt caggttgttg gcgaagttga tgcccgtgcg cagggaggat
    14761 aggaggcgga acaggggcgt ggccggctca tccaccgtgg tgttgttggg cttgcaggcg
    14821 caaaataaaa tggaaataag gagcaggtac ctgggcatcg gggtcttggt ttcggatgca
    14881 agttaatata ggcagggcat agggctatgc taaaaaaaac aagggccttt gtggatttaa
    14941 cggggcatcg gcgggtggcc attgatgtca agtttcccgg gaggctgccg ggcaaatgag
    15001 gtcagtttat atgcatggca ggcaccgatt cttcggaacc acccaggaat gaattgctag
    15061 cctattttaa tatccaatcg tgtgaaaaat ggtgcttgaa cccaacgcat agattttcgg
    15121 cttgtaacaa cgagtctatc gcatgttttg ggtttatttt tcatatgctc atactaccgc
    15181 ttgtaatcat ttgtatttgc tggtagcctt tcgtataaat tggtcgatga aaagcttggc
    15241 atccccggac tttcggcctg attaagaata actttatttt tgatcaggcc actttgggtt
    15301 taattgaaaa tatcttgtcg cccggtctat ttggtttcaa acaccaggta atcgttaaaa
    15361 aaaggcagta cttcattcaa gatccggcca tccgcagtcc atatcttatt gacatgattg
    15421 ccgatgtatt cttcggcaaa atgttcgatg ccgaggttct ccaaacgctg actcagcatg
    15481 ccgcattgga cggggaacct ggatgaatca ttcctgcccc aatccaattt aatcgccttc
    15541 agctttctta gattgccggc ataccggtca accatataaa ccggcatgtt ttggtgccat
    15601 ttttccagta cggtctggtc caccaacagg ctgtcgccct ggtaagtaaa agggacatcg
    15661 caataaaagg gcgccttatc cgggtttggg gaccaggctc gtgcaaccgc tacaagcacc
    15721 ctgggataat aggaacgttt cagttcctca tgggtcttta tccgggccag ttctttgaaa
    15781 gactcgctgt tggggccaaa ttccttgacc agggccagca agcccgggct cagagcataa
    15841 gtactgcaga atacttcagg atgaagcatc gctaatttga gcgccccgta tccacccatc
    15901 gaatggccgg caatgccgcg gccgtcccgg ttggcaatgg ttttgaaatt cagatccacg
    15961 tattccacca gttctttcgc cgtgaagtcg gcccagttgc ccgtgaggct tgaattggta
    16021 tagaaactgc cttcataaag agtatattga ctggcctgga ccaaaatgaa gggctggatt
    16081 tttttgcgat gaatggcgag gtccaggatc attttcatcc cagggtaaat actatccgtt
    16141 cccatgaaac catggagata atagattacc ggaaatcgtt tgccggcttg ttcataatcc
    16201 ggtggaaggt acacggagac tttccggttt ggattttctc ctcccgggtt ttgcaaggcc
    16261 ttggacaaca atacaaaact gaccactttg ccggcgggca tctgggccga tgaggccggg
    16321 cctgttaaaa gtgcaaggag gaatactaaa tatttcatgg aatcccgcat ggttatagaa
    16381 gggataaaat aatttatttc ttaatccttt ccaaaacccg gagctctgct ttaaacatac
    16441 caggcttcaa aaccggtttt tcccccagct ccatgatgtt ttgcgaagcg gcttcatagt
    16501 ttttccattc cggcaggccg gcgccgttag gattgcccgt ctttacaaaa ttcgaccaat
    16561 aggcggacat gtatttctcc acgccatagt cggaatcttt ccatggacgg ttccatttgg
    16621 acagggtatg cagggcaaaa ggcacttctg aagtatgaaa ggctccatag tttggaaaac
    16681 ccggtttatc cgtgggcaca tggctgaatt ggtagagata gctgttactg tcattgttca
    16741 gagcccactg gtgatccgcg tatccggcga aattcaaatt gcccaatttt agctgcgact
    16801 gcctcgcctc ttcatccgtg gcggatggga aggcatttag aaattcatct ttgtggctgg
    16861 gataggtaga agaaacccag gtcttaaatt gttccgccgt tttgaccggg ccggacaaag
    16921 ccgcatctcc ggtgacccat cctgccatca gcgaaacgtc ctgatgtttt ttgttgagga
    16981 agatggagcc caggtcttcg ggaattacgt atccgtcgat aaccggcgcg aatgttccaa
    17041 aaggcaaagt gttggctagt ttcagcaaac tgtcagcaga aagatttctc agcgcaacca
    17101 cactttctgc ctggatttta taagctaatt caaggcctga tttttcagca tctattaaat
    17161 ttttggaaaa acggcccatg gaagcgccac tgtgggcaat ggccccgcga aacagacctt
    17221 tagacaaggg cgaggccacc agggcctgta cactaaagga gccggcggat tgaccggcga
    17281 tggtgacccg gtccggatca ccgccaaact ccgtgatatt gtctttaatc caacgcagag
    17341 cggccaattg gtccaacagc gcataattcc ccgaggcatt tttaccggat tctttggtaa
    17401 ggtccgggtg cgagaagaaa ccgaaaacgc ccacgcgata attgatgctc acaaagacga
    17461 tcccttgccg ggccagggcc tccccatcgt agatggcgca ggcgctggag ccggagacga
    17521 atccaccgcc gtagatccac atcactacgg gaagtttttc tttcgcagat ttggcagggg
    17581 tccatacgtt cagatagaga caatcctcac tcaacggctc cggcggggca atgaattcct
    17641 ccgtccacat ggcaaagggt gcgggcttgt tctgataggg gcttgcaggg aatttatcgc
    17701 agaccttggt gcccgtccag ggtttgacct gttggggctc tttccaacgc agttcaccga
    17761 ccggcggtgc tgcataaggg atccctttaa aaccggatat gcttttgtct tcattcaata
    17821 ccccggacaa cttgccgctt ttggtggtga ccagggtcag ggcggtctcg ttttgcctgg
    17881 cgccggcaaa gagggtaaaa acgatggggt aaaggaggaa tattttcatt cgattttatt
    17941 gaccgttcaa tgcaatgtta taatggtttt attcggattt acaaaggttg catttttatg
    18001 gtactctttg gtgagatggt cattgactaa acgaaaccat tcattccaca aaaaacatat
    18061 tcaattcccc tttgcctttg gcgttgatct ggcccctggg cacacatttg tatttctccc
    18121 tgaccagtgc ataagtggtt tccgagatgt tgatctttcc gacttccccg ctggactcca
    18181 tccggctggc cgtattgacg gtatcgccca ggaggtcata ggcaaatttc tttttaccca
    18241 agaccccggc cactaccggg ccggaatgaa tacctgcccg cattttccat tggatactgc
    18301 tttttttgtt tcgcgcctcc aggtacctga acatctgttt tgccgcttca atgcagagta
    18361 tggcgtggtc cctgtttttt tccggcagtc cgcaggccgc gaaataggcg tccccgatgg
    18421 tcttgatctt ctcgattttg aagacttcca ggatttcgtc aaagtgacca aaaatatcat
    18481 tgagttcctt caccagttta atcggcgtga tcgacgaagc cagggaagta aaatccttaa
    18541 aatcggtgaa caggatggaa acctggtcat acttttttgc agcggctttg cctttgtttt
    18601 ttaattcatc cgcgacctgc ctgggtaata tgttgagcaa taaggtatcg gcttttttct
    18661 tctcgtccag aatagtcgct tcggcccttt tcttttctgt gatatcccgg atgatgccgc
    18721 tgtaatatct ttttccgccg tggtcccagc ttccaagcgt aagttcaatg ggaaactctt
    18781 ctcccccttt ccttagggct ttgagttcaa gcacacttcc gaggacacgg ggcttctccc
    18841 tttgattcat tcgttccatg ccttcctcat gccggtgatg caaggatccg ggaatgatca
    18901 gggtcagggg cttgcccagg gcttcctccg aagtataacg aaaaatgatt tcagccccct
    18961 tgttccagaa aaggatccgt ttgctttcat ccgaggtgat gatggcgtcg ttttcggaac
    19021 tgcagatcac ctggaacctt ttgatttgtt cctcgatcag gatttcgata tgctgctttt
    19081 ctgaaacggt tggctcggct tcaccggagt cctcgatcgg cgtatttgaa accttctggg
    19141 ccatggatgg ggtattttga ttttttgaga ttggaattgg agccgggttt ggctaatcgt
    19201 ttggttttca ctccatatgg tcaactaaag gtaatgcatc gccctgattt gaatgagcgg
    19261 ctttcccaaa ggataaggat catatagtcg ccgctaccag ttttttgtag cccgggtcca
    19321 ggcgcaggtc attgtagatg tcaaccgtca tgcggtgcgg tttagccttc gttgctcacc
    19381 aaaattatcg atgttttgtc ccgggtccta tttcctcacc gccttcagct cttcaaaaaa
    19441 gatatcatcg tttgggacgt caaattccag gccggtgacc ttcaggttgt tgtccaggac
    19501 gaaacgaacg gttcccaatt gaaaccaggc ctggggctta tcccatacca gttcgtagac
    19561 atcatagtgc cagtgacgga gggtcgcaga gaaatcgggt gtgtgttcaa agtctatttt
    19621 taactgtccg gcatgatggg tcaccttgat gttgccatag atattggcat aataggtgcc
    19681 ggcataccgg tctgcgggca gggtggactg agtacccatg accctcgctt ttttacggtc
    19741 ttcgatgcgc gtatccgatt ttttgttgcg ggccaggttt tcaagttgtt ctttggacca
    19801 gtcccgggag tctttgccca ggaaggcatc aaaggtatag ttgggcaggg ctccgatggg
    19861 ggcttgcagg ccattggtca gcatgatgaa accgaggttt tcatcgggca gcatttggat
    19921 ggtggagatc atcccgtcat agccccccgt atgtcctatc cgcattttgc cctggtagtc
    19981 ggagatgccc cagccgagtg cataaccgcg gaaatgggtc agggcatttt tttcggtatg
    20041 gtctaccgga aaattgttgt ggggcgtcca aagggtgttc cgggaagttt tggtcagcag
    20101 ggtatccttg ccccggatgc cgtggttcat attcaggatc agccactggg caaggtcctg
    20161 cacagaagaa atcaatcctc cggttgcggc caccgtttcc cagtccaccc agggtatggg
    20221 cttgtttttg ccatccagga attcatgggg tgtggctaca ttgctcaggc cgggcaggaa
    20281 gcgggtcgca tagacggtcc tcgacatacc gaggggtttc aggatttttt cggtgatgta
    20341 ttccccccag gttttcccgg tgacggtttt aatgatttct cctgccgtga catacatgac
    20401 attgctgtac ccgtaccctg acctgaaatc ataggctttg gggaggtatt taagccggcg
    20461 gatgatctct tcggccgtca gatcggagcg gtaccagatc acatccccgc tgaaagtgcc
    20521 gagtccgacc cggtggcaaa gcaggtcgcg gacggtggtt tcatcgctta catagcggtc
    20581 gtacaattca aaatagggga ggtatttttt tactttatca tcccaggcga tttttccttc
    20641 ctggaccagc atggctatgg ctgctgaagt gaaggccttg gaattggagg caatggcgta
    20701 cagcgtattt tcatcgggtg tttgattttt ccctgcctcc atggtcccgt agcctttggc
    20761 aaaaaccagt ttgccgtcct tcacaatacc gatgctcatg ccggggacat tccagtcctg
    20821 gagggctttt tggtagtaag cgtccagttt gttcagatca ggctgagccc acagggtttt
    20881 gcagcagata aggaggaggg cgatcaaggc aagtgatttt cccaccaggt tttcgtggaa
    20941 ttttttcata agggagtgat tattatctgg tgtaagatat tctgtttcgc gggtttcccg
    21001 cgaatggatc tgcgtgggtt gctaagcggg tttcccgcta agatttccca caaagacaca
    21061 gcgaagcgaa ggacacaaag gaataggaaa tcccatctca tttttctttg tgggcttcgt
    21121 gtcttggtcc cgctaagatt tcccacaaag acgcaaagag cacaaaggaa taaagtcatc
    21181 ttatcgcatt tttctttgtg ggcttcgtgg cttggtggga gccaatgggc cgctgtgcgg
    21241 ctttcccgct aagatttccc acaaagacac agcgaagcga agagcacaaa ggaataagtc
    21301 atcttatccc atttttcttt gt
//
LOCUS       DAOQXT010000081        21084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2880_length_21084_cov_5.936374, whole genome shotgun sequence.
ACCESSION   DAOQXT010000081 DAOQXT010000000
VERSION     DAOQXT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 21084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21084
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2880_length_21084_cov_5.936374"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..948)
                     /locus_tag="PKM27_13635"
     CDS             complement(52..948)
                     /locus_tag="PKM27_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08354.1"
                     /translation="MRILFLLLFWLATIPLSNAQCKAIRVNSTLINQTGSSNGYYNYT
                     ISANVTLEYGNASIIPYYRCGSGEEMALDDCFTWESPSTRDFTSDVFRCPCDQPVSIR
                     LEGYASSNCKGNTCGNVEGSESNLALDLTDFRIAGHDADQVCFKWFIDPEEKETRYQI
                     ESSIDGIHFTYLTTFTSPDILLSGTAASKCMVRTNYNPYYRMKAIEKSGRALYSPIRS
                     INGRPAQTRILYNMNTREITLSGPDDQLASCVLHVYDAKGRRVYEEVIKGDKVLLPEF
                     PEGIYFIQINRSCDALVKKIVL"
     gene            complement(1134..2051)
                     /locus_tag="PKM27_13640"
     CDS             complement(1134..2051)
                     /locus_tag="PKM27_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08355.1"
                     /translation="MKLLYTVLLWSITSVIVYSQCSAVEVNNSSILQTGSSGGNCTYI
                     VNANITLQRGNASVKVFYTCGTNATEMVLEDCFSWDKPSTKNFTSSSFTCPCEAEVSV
                     RFIGYANPSCGGNACGEVGTINSGESTESQLALDLTNFKVEGLIRDQACFSWEVEPTE
                     TGTVFQLEKSTDGKSFVALTSLSYRDIRIQGTTATYCMPLRGTAQYFRIKAVETTGKV
                     HTSPVRFVKNKSAFTEVQFSPVSHQIRLVGDVDDLMAKPLYIYSAGGQLVHQQTINSD
                     RIDLPQLVGGIYIVRIPQAEGPLVRKVII"
     gene            complement(2270..3163)
                     /locus_tag="PKM27_13645"
     CDS             complement(2270..3163)
                     /locus_tag="PKM27_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08356.1"
                     /translation="MKYLCTVALWGISFIAGYSQCKQIFYSAVSLDLISSSGGNNTYT
                     ITATFTLNNGNASVKPYYTPGGGAEVALADCWNFPTPSTQTYTSSQFTVPSNATVSLR
                     FIAHANPNCGGTTCEILPAGSQLALDLTQFKISDVVRERACFNWHVELQEPNTLFHLE
                     RSMDGKHFSTFATLTFNEISLQGSEASYCANHLGVENYYRLKAVSNEGLTLFSPIRYL
                     NIQTSRVDIQFSPLSRQIRLSGGVDEVFSKPLSVINAYGQVVFQEYIKSDRTGLPELP
                     KGIYFVRIEKEGESILKKIAL"
     gene            3367..4182
                     /locus_tag="PKM27_13650"
     CDS             3367..4182
                     /locus_tag="PKM27_13650"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNR08357.1"
                     /translation="MESLYIRINQAWDDASLTGAPEVLEAIQETLALLDQGKIRVAEP
                     GLGGWTVNEWVKKAILLFFRISKMETIQNGPFEYYDKIPLKKNFAALGVRVVPQALAR
                     YGSFIEAGAILMPSYVNIGAYVGSGTMVDTWATVGSCAQIGRNVHLSGGVGIGGVLEP
                     PQAAPVIIEDHAFIGSRCIVVEGVKVGTEAVLGANVVLTQSTHIIDVSGPQPVTYRGE
                     VPPRSVVIPGSFDKSFPAGTFQVPCALIIGTRKESTDKKVSLNDTLREFNVAV"
     gene            4228..6423
                     /locus_tag="PKM27_13655"
     CDS             4228..6423
                     /locus_tag="PKM27_13655"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNR08358.1"
                     /translation="MRNRTILCFLFIGIFLHTTRADEGMWLPQLLSVLNESRMKSLGM
                     KISAKDIYNTNTGSLKDAVVSFGGFCTGAVISSNGLVLTNHHCGFDAIQNHSTLDKNY
                     IRDGFWAQTRADEIPNPGLFVTFIVRIDDVSREALRGLSPSMTESERQGLIDKNLAEL
                     TKNVKKESGQESFTRGFFEGNQYYLFVTETYRDVRLVGAPPSQIGNFGKDTDNWVWPR
                     HTGDFSLFRIYASRDNKPAEYAPDNVPYTPKKSFDISMDGVEPGDFTFVFGFPGRTMQ
                     YLHSEAVRQILEVSDPAKIGIRERALKILDGFMREDEQVKIQYAAKYAGIQNSYKKWQ
                     GEVLGLNRAGAVGKKQKYEALFQQRVDADAGFRQRYGSLLNELSKGYESYEPYGLASD
                     YYLEIISKIEMFTIASQLNSLVTAFEKGGENGYRQRLPVVKSNLENILGEFSGKVDRR
                     MFEELMDMYVQQPGGYVSPLLKERLKAAGGNYINLSLTLYGQTSLFDMDQLNSLFARS
                     AGETVEAIRRFEAVQLFVDLQKTYQSLVQSPSAEIQNRINRLQRDYMQAQLEVMKEKK
                     FYPDANRTLRVTFGQVQGYSARNAVQYHYYTDLDGVMEKYKPGDYEFDVPEKLRALHA
                     AKDFGPYGSEGVMPVCFIATNHTTGGNSGSPALDARGNLIGLNFDRAWEGTMSDIHYD
                     ASICRNIMVDIRYVLFIIDKYAGAGHLIREMKLVHPKKGPKKKKSKAGL"
     gene            complement(6447..6920)
                     /locus_tag="PKM27_13660"
     CDS             complement(6447..6920)
                     /locus_tag="PKM27_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNR08359.1"
                     /translation="MAEELKILTGTREEQYRALIPQIKALLEGEPDQIANMANVCAAL
                     KAQFEWLWIGFYLVRNEQLVLGPFQGPVACTRIGYGKGVCGTAWARGETVLVPDVEQF
                     PGHIACSSLSRSEIVVPLLRDGRVHGVLDIDSIELDAFNKTDQRYLEEIVGLLTS"
     gene            complement(7002..7484)
                     /locus_tag="PKM27_13665"
     CDS             complement(7002..7484)
                     /locus_tag="PKM27_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNR08360.1"
                     /translation="MKHQTQTFPEPLGPYSALRISSTAFKEGEFIPSRYTCDGHDVNP
                     PLSVHSVPEKSKSLVLIVADPDCPGGTWIHWLVWNIPPGASIAENEIPGEEGTNDFKK
                     VHFGGPCPPSGVHRYYFKIYALDCLLNAAPGSGIRQVEKAMEGHVLAFGQLMGRYRRH
                     "
     gene            7770..8294
                     /locus_tag="PKM27_13670"
     CDS             7770..8294
                     /locus_tag="PKM27_13670"
                     /inference="COORDINATES: protein motif:HMM:NF017758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HNR08361.1"
                     /translation="MELNHSPMVTLNHLLDYDINQFYLAEVQLSHHLPVLIERAGSLT
                     FKTILQKYLDYVRQHVQKFEGFLAEGKGGLPEPGNRIMEAFLDEAEDRLVQCSDPEVK
                     DACLLASVQSINHYKISAYGTAASFANALGMHNKAHIFHDAEVNEKQIDERLTQLAEQ
                     EINIRAKAPFLLHQ"
     gene            complement(8346..8717)
                     /locus_tag="PKM27_13675"
     CDS             complement(8346..8717)
                     /locus_tag="PKM27_13675"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNR08362.1"
                     /translation="MGYQFLNIFFFVFHSGLILFNLTGWIFPKTKKLHLITVGLTAFS
                     WFILGIWYGWGYCYCTDWHWEVREHLGYEDPPDSYIHFLISKLLGLELDPKLVDRGTL
                     ILFLIIFAASVWVNRKRSSQP"
     gene            complement(8723..9202)
                     /locus_tag="PKM27_13680"
     CDS             complement(8723..9202)
                     /locus_tag="PKM27_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNR08363.1"
                     /translation="MKKSAGILLFRLADERPEVFLVHPGGPFWVKKDEGSWSIPKGEF
                     GENENPLDAARRELQEETGILAEGSFIELTPVRQKSGKIIYPWALQQDADPLSITSNF
                     FEMEWPPKSGIKQSFPEIDRAAWFEFPEAKKKLVPGQVAILDELEKKLLQAKGMFYK"
     gene            complement(9199..10644)
                     /locus_tag="PKM27_13685"
     CDS             complement(9199..10644)
                     /locus_tag="PKM27_13685"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNR08364.1"
                     /translation="MSKEYPLNRLGPVLWEIPMSAHPAMRVPGHIYCREELLDAIRHD
                     RALEQLINVASLPGIQRAAIVMPDAHEGYGFPIGGVAATRWPDGFISPGGIGYDINCG
                     VRLLLTPLVFRDVENRVEAFSKDLYSSVPSGTGKGGDVLLNLNEMNAVLRQGAAWAVS
                     HQLGEPEDLEFIESNGCLANADPEKVSEQAKRRGLDQLGTIGSGNHFVEMDRISAIFN
                     DQAAAAFGLSQDQIVILIHTGSRGLGHQVATDYLRLMVNVMDQYGFKPRDRELAGVPF
                     NAPEGQSYFNAMAAAANFAWCNREIISHKIRQSLQRVFNLNPGHVKLLYDVAHNIAKI
                     EDHPVAGVPTKLIVHRKGATRAFGPGHLDIPEKYRAIGQPVLIPGSMGTSSFVLAGAP
                     NSMDSAFGSTCHGAGRKLSRTAAKKQVGGPELKEALRKEGIYIQAGSFGGIAEEAPLA
                     YKDVDLVVDTVHEAGLAIKVARLKPLAVIKG"
     gene            complement(10654..11070)
                     /locus_tag="PKM27_13690"
     CDS             complement(10654..11070)
                     /locus_tag="PKM27_13690"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNR08365.1"
                     /translation="MPYSFYPHTADIRLKVTGSTRGEVFGLAVQAMADLIKEGFCKKD
                     KPDFSREERLELKSIDGTSLLVDFLSEVLTLSHIRKTIFCDVIFDTLAETELKAILRG
                     KATDQFDKDIKAVTYHEAELKKSENGQWESILVFDI"
     gene            complement(11101..12552)
                     /locus_tag="PKM27_13695"
     CDS             complement(11101..12552)
                     /locus_tag="PKM27_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNR08366.1"
                     /translation="MKWFPILCLSLLFLIACKDQGKREILPALNAGFSSFEERQKNSG
                     RDETAVYTQTAASGIHYRLAARRATPGVVHIKSVYPVNEQRQLPDFFRDFFGEDFWRE
                     YNSPQMRMGSASGVIISDDGYIATNNHVVADAQSIEVVLYDQRSYQAKTIGVDPATDL
                     ALLKIEEKNLNFIEFGNSDSIEVGDVVLAVGNPFNLASTVTAGIVSAKARNINIISDQ
                     AAIESFIQTDAAVNPGNSGGALVDVYGRLVGINTAIATPTGTYAGYSFAIPVEIVKKT
                     MDDLLRFGKVVHAYLGVFISDMNGEKAKLLGIRQTTGVLVDSLQSLGAAKRAGVQKND
                     VIIEAGRRSVGTSTQLREIIARHGPGETLELRVIRNGEEKIIPVTLLPETETRQSEAA
                     LLKVLGIEIENLSPQERRNLNIEGGVKISRITKGALSGTGIKTGFIVLRVNGKSVNNR
                     NEFLDELRDKQGGMMIEGIYPGSPGLFYYAFGL"
     gene            complement(12777..14324)
                     /locus_tag="PKM27_13700"
     CDS             complement(12777..14324)
                     /locus_tag="PKM27_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter TolR"
                     /protein_id="HNR08367.1"
                     /translation="MRFKSIFFLGIFFIAIAPAKAQSKGFGVFDTAVDIGKPKISGSS
                     AYDAENQAYHIKGAGYNIWFNRDEFHFLTKRIQGDFIVTADFEFPAAGKEAHRKVGWM
                     VRENLNDDAAHLSATYHGGDGLTVAQWRVMRGAYMRDPQDEIFFPKKRARTIQLERKG
                     KTYTMRIANWGEPLQSAGSQVMENIGDNPYVGIFICSHNADVLEEARVWNVRIDRPMP
                     PNFNFGGADTIGSRLEVLDVFTGKRRVVYESPVRFEAPNFMPDGKSLLINSKGSLWTV
                     LLSGKGSPVKFNTGFADRNNNDHVISFDGKMVGISHHRTGMFGGGSTVYVVPITGGIP
                     KLVTEKTPSYLHGWSANNKEVYYVGRRDTTRNSPYHVYKANIETGAETQLTRLPFGHV
                     DGPEGSPDGQWIYYNSSQTGTMQLWRMKPDGTGNEQLTFDEYNNWFPHISPDGKWIAY
                     ISFPVDIDPVSHPACKAVSLKLMPVAGGAPRTIAYLYGGQGTINTPSWSADSKQIAFV
                     SYSGRIK"
     gene            complement(14425..16293)
                     /locus_tag="PKM27_13705"
     CDS             complement(14425..16293)
                     /locus_tag="PKM27_13705"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR08368.1"
                     /translation="MKRLYIHSLFTLSGLLLAVNFGCNDFLDKQPLGALSASNVTNKS
                     GISALLIGAYSLLDGQVGLTNLAGVAGVVNPTGITYASACSNWIYGSVCADDSYKGST
                     PTDQPPIVQLATWSLSATGGTNNYMNYKWVILYTGIQRANEVMRLIPQATDIDAAEAS
                     RMIAEARFLRAHYHMDAQKMWNNPPYVDESVSLSNNNLNVPNIDDAGNFVNIWPKIEA
                     DLAFARDNLPATQAQAGRVNKWAAQAYLAKAYMFQDKFAQAKPLLEDLIANGVTAKGE
                     KYSLVNFESNFNAQTKNGAESVFACQMSVNDGSQLNGNFGDVLNFPNGSGAPGGCCGF
                     NNPSFNLANAFKTDENGLPLLDTWNEGKGVNDENPANVYDGPLDPRIDWTIGRPGIPY
                     LDYGPHTPAWIRDLSVNGVFSPKKNVYAKSQFGSLSSNETAFWGATQINANNYIFIRF
                     ADIILWAAECEVETGSLDKAQEYVNLVRNRAADPSGWVYKNAAYSATAAKYTVTAASV
                     PADNYKISAYPAGRFASMGKDYARKAVQFERRLELAMEGHRFFDLQRWDNGTGSMANI
                     LNAYQAVEKTKKSFHSVNPTARFTKGASEFFPLPQTQIDLENSSGKMVLKQNPGYN"
     gene            complement(16317..19514)
                     /locus_tag="PKM27_13710"
     CDS             complement(16317..19514)
                     /locus_tag="PKM27_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR08369.1"
                     /translation="MKRYVALILFLSLSCQFSYAQRGSLQGTVSGDGEPLEFANVGIT
                     ELGLGSSTDASGKYLIQDIPFGTYTLTVSYVGFETHSLSVTISSTNPLTVDIAMGAGT
                     VLDEIVVTGYTAQKKKDITGAVAVVDMKNLRAVPSGTIESVLQGNAAGVTVINSGAPG
                     SPSNVRVRGITSVGSTDPLVIIDGTPGSMHDLNPNDIQSIQVLKDAGAAAIYGVRGSN
                     GVIIITTKRGQAGKITVSYDTYIGTQRPLKDGWNLANPTENANAIWEQFKNSNLPPIH
                     AQYGNGATPVIPDYITPTGQVGESAKTDPATYALYSNQITKANKAGTDWFHEIFKPAT
                     VMNHDLSISGGSERATFLLSLNYLDQDGTLIRNYLKRYGARINSAYNVSKSIRIGQSA
                     YFNYKKNPGYLGLPGINNANSINAAFRMPAIVPVYDIKGNFAGGGSRSLGNSPNPVAI
                     MERTKDNKANNYGILGNVYAEVDFLKNFTVKTSFGGAVDLFNNYSFVFTAYENAENAQ
                     NPNSFIENYGSVTSRTWTNTLNYKNTFAGSHNLNVLIGSEAISNAGRGISGARGSYYV
                     TNPANLTVDPNLWTLNFGTPTGQSNSNIAINGFQTPYENALFSLFGRLDYNFENKYYL
                     SGTLRRDGSSVFAPENRYGVFPSVTVGWRISAEPFMKGISWLDDLKLRGGWGKLGSIS
                     NVSPTNAFTLFSQSSVESYYDINGTSTTPVQGLFASQFGNVNTTWEEDIITNIGFDAS
                     LLKGRFDLSAEWYKKEISGLLFPAAIPATAGGSRPPFVNSGNIQNSGVDLSLTYHGKL
                     GEDLSFDIGGNFTSYANEVKSVPPGFLYIDYGQSRLQPGHPIGAFFGYKQIGLFQSQA
                     EVDAAPKQDAAAPGRHRFEDINGDNKIDANDRTHFGNPNPDFTAGLTLGATYKNLDLS
                     MFWYANVGNEVINQVRNSIDFPQQFDVAISKDAVYNSWRPDRPNAKVPRLERGANFSN
                     VTAFNSYLMEDGSFVRLKNLQLGYSVPVSKLDRIGASRLRIYVQAVNLFTFTKYTGLD
                     PELLSSTLTSTNFGIDGGAYPANQKMFTGGLNITF"
     gene            19734..20816
                     /locus_tag="PKM27_13715"
     CDS             19734..20816
                     /locus_tag="PKM27_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR08370.1"
                     /translation="MEDANSNKAPDLKKKKTHVTIHDLAAELKVSPSTVSRALHGHPG
                     ISQERIKAITRLAKKRNYVPNELAAGLRQSRSNCIGVITTWIDRNFHSSLIRSVEEEV
                     SSKGMNVIILQSHDRYKDEVENAKALLNSRVSGLIVSLAMETRDYSHFKPFFERQIPV
                     IFADRVPREIHAHKVLVDNFKAAYQATSHLIEQGYRHIAHLSGQQHRDNYLDRQEGYV
                     QALKDHGIRPDKRLIYECETKSAEEGVLAVQQLLSLKQPPDALFSTNDTMAVSILQYF
                     KKIGIRVPRDFGIVGFNNDPITSIIDPALTTTYNPAYEIGKHAAKLLLEHQRQNWTEK
                     DFKTIRLETELIVRESTDKKGLRNEG"
BASE COUNT         5140 a         5361 c         5394 g         5189 t
ORIGIN      
        1 gttaatagtc aagagtcaag agtcaataat caatagtcag aataccaaca gctagagaac
       61 tatttttttc accagcgcat cgcaggaccg attgatttga ataaaataga tgccttccgg
      121 aaattccggg agcaagactt tatccccttt gatcacttcc tcatacactc tccggccctt
      181 ggcatcatag acatgcagga cgcaggaggc gagttggtcg tcgggccccg aaagcgtgat
      241 ttcccgcgtg ttcatattgt agaggatccg ggtttgggcc ggacgtccat ttatacttcg
      301 gatgggagaa tacaaagccc tgcctgactt ttcaatcgct ttcatccggt aatagggatt
      361 ataattggtc cggaccatgc atttggaagc tgcggtgccg gacaacagga tgtcgggcga
      421 ggtgaaagta gtcaggtagg taaaatgaat gccgtcaata ctcgattcaa tctgataccg
      481 ggtttccttt tcttccggat caataaacca tttgaaacaa acctgatccg catcgtgacc
      541 tgcaatcctg aaatccgtga ggtcaagggc gagattggat tccgaccctt ccacattccc
      601 gcacgtattg cctttacaat tggaactggc atagccctca agccggatag aaaccggttg
      661 gtcacacgga caccggaaaa cgtcactggt aaaatccctg gtagacgggg actcccaggt
      721 aaaacaatca tccagggcca tttcctcacc gctcccacaa cgataataag gtattatgga
      781 agcattgccg tattccagcg tgacatttgc ggaaatggta taattataat atccgttcga
      841 actgccggtt tggttgatca gggtactatt gacccggata gctttgcatt gggcattact
      901 caaaggaatg gttgcaagcc aaaacaagag caaaaataaa attctcataa ttaaatttac
      961 atgctttaca agcctaaggc cctgatttca atcacgcctc ttaatgatgg ctaaagccag
     1021 cttttcttgg taattacgta attaccaaag ccgtacaaat caagtcgcat cagaaggaca
     1081 tcgacccgca aacttgcaaa cttgcaaact tgcaaactaa cagaactaac agactaaata
     1141 atcaccttcc tgaccagggg gccttcggcc tgaggaatac gcacgatata gatcccgccc
     1201 acgagttggg gcaagtcaat ccggtcggaa tttatagttt gctggtggac cagttggccg
     1261 ccagcggaat agatgtacag aggtttggcc atcaggtcat ccacatctcc aaccaggcgg
     1321 atttgatggc tgaccgggct gaactgcact tcggtgaaag cacttttgtt ttttacgaaa
     1381 cggacggggg aagtatgtac tttccctgtg gtttcgaccg ccttgatcct gaaatactgg
     1441 gccgttcccc ggagaggcat gcaataggtc gcggtggttc cctggatccg gatatcccta
     1501 tagctcaggg aggtcagggc cacaaaggat ttcccatccg tgcttttttc cagttgaaaa
     1561 acggtgccgg tttccgtcgg ctcgacttcc cagctgaaac aagcctggtc cctgatcaaa
     1621 ccttcgacct taaaattggt caggtccagg gccaattggc tttcggtgct ttctccgcta
     1681 ttgatcgttc cgacttcacc gcaggcattt cccccacagg aagggttggc atatccgatg
     1741 aatcggactg atacttcggc ttcgcaggga caggtaaaag aggaggatgt gaaatttttt
     1801 gtagagggtt tgtcccagga aaaacagtct tccaaaacca tctcagttgc attcgttccg
     1861 caggtataga ataccttcac ggaggcattg cccctttgca gggtaatgtt cgcatttaca
     1921 atataggtgc aatttccgcc ggaacttccg gtttgaagga tactggaatt attaacttca
     1981 acggcggagc attgtgaata cacaatgaca gaggtgatac tccacaggag taccgtgtac
     2041 agcagcttca taaatttagt gtaaaagggt taacttttaa cggcgcaaat atagcgtatc
     2101 taccccccca agaagtgtaa cttaggttac aaactttgaa ataaaaatgt taacgaatag
     2161 ttaacgatcg taaaaccttg ttatctctaa tactatactt tatgagtgtt tataatatcg
     2221 cccgtccggc gactgcttat ttcaataatt gtttggtgaa gatcttaatt caaagtgcaa
     2281 ttttcttcaa gatggattcc ccttcctttt caatccgtac aaaatatatt cctttgggga
     2341 gctccggcaa gcccgtccgg tcagatttta tgtattcctg gaaaaccacc tggccataag
     2401 cattgatcac ggagaggggc ttggaaaata cctcatccac ccctccggac aatctgattt
     2461 gacgggagag cggactaaat tgaatatcca ctcttgaggt ctgtatgttt aaatatcgga
     2521 tgggcgaaaa aagggtaagc ccttcattgc tcacggcttt cagcctgtaa taattttcaa
     2581 ctccgaggtg gtttgcgcaa taggatgctt ccgaaccctg gaggctgatc tcgttaaaag
     2641 tcagggttgc aaaagtggag aagtgcttcc catccatgct tctttccaaa tgaaacaggg
     2701 tattgggttc ctggagttcg acatgccagt taaaacaagc cctttcccgg acaacatctg
     2761 aaattttaaa ctgggtaagg tccagggcca gttggctgcc tgcggggaga atctcacagg
     2821 tggttcctcc gcaattcgga ttggcatggg caatgaacct aaggctgact gttgcattgg
     2881 acggaacggt aaactgggaa gaagtataag tctgggtcga gggcgtcgga aaattccaac
     2941 aatcggccag ggccacctcc gcccctccgc cgggggtata atatggtttt accgaagcat
     3001 tcccgttgtt aagggtaaag gtggcggtga tggtataggt attgttcccg ccggaagaag
     3061 aaattaaatc aagggaaact gctgagtaga aaatttgttt gcattgggaa taccctgcaa
     3121 tgaaggatat accccacagg gctactgtgc aaaggtattt cataatttag tataaagggt
     3181 taaaaaactg ctgcaaagat atacgaaaat ccctttaaac ttacaaatcc tcaccggcct
     3241 accttccccg ggaacacata accctggatt tatcaggaaa cgtaaaccca ttttcatcca
     3301 atggaaggag tgactgttct gcccaaatta tttcatggtc cactccaatg tctatctttg
     3361 tactccatgg aatccttata tatccgaatc aaccaggcct gggacgatgc ctcacttacc
     3421 ggagcccccg aagtgttgga ggccatccag gaaaccctgg ctcttctcga ccagggtaag
     3481 atccgggtgg cagaacccgg gcttgggggt tggacggtga atgaatgggt taaaaaagcc
     3541 attcttttat ttttcaggat cagtaaaatg gagacgatcc agaacggccc ttttgagtat
     3601 tacgacaaaa ttcctttgaa gaaaaacttc gcagccctgg gagtccgggt ggtgcctcag
     3661 gccctggccc gctatggttc attcattgaa gccggagcca tcctgatgcc cagctatgtc
     3721 aacataggcg cttatgtagg cagcgggacc atggtggata cctgggccac cgtaggcagt
     3781 tgcgcccaga ttggaagaaa tgtccatctc agcggcggag tgggtattgg cggggtactc
     3841 gaaccacccc aggcagcgcc ggtcatcatt gaggaccatg cctttattgg ttcccgttgt
     3901 atcgtcgtgg agggcgtcaa ggtcggaacg gaagccgtcc tgggggccaa tgtggtgctt
     3961 acccagtcca cccacatcat tgatgtcagc gggccgcaac cggtgactta tcgcggagag
     4021 gtgccgcccc ggtcggtggt gatcccgggt tcctttgaca agtcctttcc tgcgggaact
     4081 ttccaggtgc cctgcgccct gatcatcgga acgcgaaaag aaagcacgga caaaaaggtt
     4141 tctttgaatg acaccctgag agaattcaat gtcgccgtat gagcgctatt tgctatggca
     4201 acaagtataa ttttttaacc atctaatatg cgcaaccgaa ccattctttg ctttttgttc
     4261 atagggatct tcctccatac taccagggca gatgaaggga tgtggctgcc ccagttactg
     4321 agtgtgctca atgagagcag gatgaagagc ctgggtatga aaatcagcgc gaaagacata
     4381 tacaacacca atacggggag ccttaaagat gccgtggtaa gttttggggg cttctgcaca
     4441 ggagccgtca tttcttccaa tggtttggtg ctgaccaatc accattgtgg ttttgacgcg
     4501 atacaaaacc acagcaccct ggataaaaat tatatccgtg atggtttctg ggctcaaacc
     4561 agggcggatg aaatccccaa cccgggcctg ttcgtcactt ttattgtccg catcgacgat
     4621 gtgagccggg aagccctgcg cgggctgtcc ccttcgatga cggaatccga acgccagggc
     4681 ctgattgata aaaacctggc ggagttgact aagaacgtga agaaagaaag cggccaggaa
     4741 agttttaccc ggggtttttt tgaaggcaac caatattacc ttttcgtcac cgaaacctac
     4801 cgggatgtgc gcctggtcgg ggctcctcct tcccagatcg ggaattttgg caaggacacg
     4861 gacaactggg tctggccaag gcatacggga gatttttccc tgttccgcat ctacgcctcc
     4921 agggataaca aaccggcgga atatgcaccg gacaatgtac cctacacccc caaaaagtcc
     4981 tttgatattt ccatggacgg agtggagccg ggtgatttta ctttcgtgtt cggatttcca
     5041 ggccgcacta tgcaatacct tcacagcgaa gcggtacgcc agatccttga ggtcagcgat
     5101 cccgcaaaaa tcgggatccg ggagcgggca ttaaaaatac tggacggctt catgcgcgag
     5161 gacgaacaag taaaaatcca atatgccgcc aaatatgcgg gcatccagaa ttcctataaa
     5221 aaatggcagg gcgaggtgtt gggactgaac cgtgccgggg ccgtcggaaa aaaacaaaaa
     5281 tacgaagccc tgttccagca acgggtggat gccgatgccg gcttcaggca gcggtacggc
     5341 tcactcctga atgaactgtc caaggggtat gaatcctacg agccctatgg tctggccagt
     5401 gattattacc tggaaatcat cagcaaaatt gaaatgttta ccattgcctc ccaactgaat
     5461 agcctggtta cggctttcga gaaagggggg gaaaacggat accggcagcg cctgcccgtg
     5521 gtcaagtcca atctggaaaa catcctggga gaattctccg ggaaagtgga ccggcggatg
     5581 tttgaagagt tgatggatat gtatgtgcaa cagcccggcg gctatgtttc ccctttgcta
     5641 aaagaacgcc tgaaggccgc gggaggcaat tatatcaacc tgtccctaac cctttatgga
     5701 cagaccagcc tgtttgatat ggatcagtta aacagcctgt ttgcccgatc cgccggagag
     5761 acggtggagg ctatacgccg ctttgaagcc gtgcaattgt ttgtggactt gcagaaaacc
     5821 taccagtccc tggtgcagtc cccttctgcg gaaattcaaa accgcattaa ccgcttgcag
     5881 cgggattata tgcaggccca gctggaagtg atgaaagaaa aaaagtttta ccccgatgcc
     5941 aaccggaccc tgagggtgac cttcggacag gtccagggtt attcagccag gaatgccgtc
     6001 caataccatt attacaccga cctggatggg gtcatggaaa aatacaaacc cggcgattat
     6061 gaatttgacg taccggaaaa actgagggcc ctgcatgccg ctaaagactt tggaccctat
     6121 ggctcagagg gcgtaatgcc ggtttgtttt attgctacca accataccac gggcggaaac
     6181 agcggcagcc cggcgctgga tgcgcgggga aatctgatcg gactcaattt cgaccgggcc
     6241 tgggaaggca ctatgagcga catccattac gacgcctcca tctgccgaaa catcatggtg
     6301 gatatccgct atgtactctt tatcattgat aaatacgcgg gtgccggcca tttgatccgg
     6361 gaaatgaaac tggtccaccc caaaaaaggc cctaaaaaga aaaaatcaaa agccgggctg
     6421 taaaaccgca aaaggggccc tcgctttcag gaagtcaata aacccacgat ttcctccaga
     6481 tacctttggt cggttttatt aaaggcgtcc aattcgatgc tgtctatatc cagtacccca
     6541 tgcacccggc cgtcccggag aagggggact acgatctctg acctggaaag actgctgcaa
     6601 gcgatatgac cggggaattg ttccacatcc ggtaccaaca cggtttcacc cctggcccag
     6661 gctgtgccac atactccttt cccatatccg atgcgggtgc aggcgaccgg cccctggaaa
     6721 ggtccgagga ccaattgttc attccggacc aggtaaaatc caatccaaag ccattcaaac
     6781 tgtgctttga gcgcggcaca gacattggcc atattggcaa tttgatccgg ctccccctca
     6841 aggagcgctt tgatttgagg aatcaaggcc ctgtattgtt cttcccttgt tcccgtgagg
     6901 atctttaatt cttcagccat gttttcaaag gtacaattcc ttgtctttgg ggatggaatc
     6961 gtctagactt aagccgacca aagcgaacct tagtcccacg gctaatgcct acgatatctg
     7021 cccataagct ggccaaaggc cagaacatgc ccctccatcg ctttctccac ctgcctgatc
     7081 ccggaaccgg gggccgcatt cagcaggcaa tccagggcat agatcttgaa ataatacctg
     7141 tgcaccccgg acgggggaca gggtccgcca aaatggactt ttttaaaatc attggtcccc
     7201 tcctctcccg ggatttcatt ttcggcgatg gacgctcccg gtgggatatt ccaaaccagc
     7261 cagtgaatcc aggttcctcc cggacaatcc gggtctgcaa cgatgaggac caggcttttt
     7321 gatttttccg gaacggaatg aacggacaaa ggcggattca catcatggcc gtcacaggta
     7381 taccgggatg gaataaattc cccttcctta aaagcggtac tgctgatcct taaggcagaa
     7441 tatggtccaa ggggttcagg aaaagtttga gtctgatgtt tcatgaggta tagtgatttc
     7501 aggtggtaaa atccggatgg gttcttcttc aggaaccggt ttgaagggct cttctttctt
     7561 caccggatcc aggggctctg caggaggctg gatttccgac gcagcaggtg gagcgggtat
     7621 ttcgggtcct ttttcccttt cttttcccat attatgactc acaatctatg ggatattcaa
     7681 gtccacaatc ttaacatgaa tcaaaggaat ctttgacagc cgtcattgcc ctggcccggg
     7741 gtttgaatgt aaattgaaca aaaaaaaata tggaactgaa tcattcgccg atggtcactt
     7801 tgaatcattt attggattat gatatcaatc aattttacct tgcagaagta caattaagcc
     7861 accacctccc ggttttgatc gaaagggccg gttccttaac ctttaaaacc attctgcaga
     7921 agtacctcga ttatgtccgg cagcatgttc aaaaatttga aggtttcctg gcggaaggga
     7981 aagggggtct gcccgaaccc ggaaaccgga tcatggaagc tttcctggat gaagccgaag
     8041 accggctggt ccagtgttct gatccggaag tcaaagacgc ctgcctgctg gcttccgtgc
     8101 agtctatcaa tcattataaa atcagcgctt acggcaccgc tgcttctttc gccaacgccc
     8161 ttgggatgca taacaaggcc catatcttcc acgatgcgga ggtaaatgaa aaacaaatcg
     8221 atgaacgttt aacccaattg gccgaacaag aaattaacat aagggccaaa gcgccttttt
     8281 tgttgcacca gtaaaggttg aggttgaggt taaggttgag gttaaggtta aggttaaggt
     8341 tgaggttaag gttgagagga cctctttctg ttcacccaga cactggccgc aaaaatgatc
     8401 aggaagagga taagcgttcc ccggtctacg agtttaggat ccagttcaag accgagaagt
     8461 ttggatatca aaaaatggat ataggaatcg ggagggtctt cataccccag gtgttcgcgc
     8521 acttcccaat gccagtctgt gcaataacaa taaccccatc cgtaccatat gcccaggata
     8581 aaccacgaaa atgccgtcag gccgacggtg atcaggtgca gtttttttgt tttcgggaat
     8641 atccagccgg tcagattaaa gaggataagg ccactatgaa aaacaaaaaa gaagatgttc
     8701 aggaactggt accccattcg gatcatttat aaaacattcc tttagcttga agcagttttt
     8761 tttcaagttc atccaaaata gccacctggc cgggaaccag cttttttttt gcttcaggaa
     8821 attcaaacca ggctgcgcgg tcaatttctg gaaaggattg ttttattcct gatttcggcg
     8881 gccattccat ttcaaagaag ttactggtga tggacagggg gtcggcatct tgttgcaaag
     8941 cccagggata aatgatcttg ccggatttct ggcggaccgg ggtcagttct atgaaagagc
     9001 cttccgccaa aatgcctgtt tcttcctgca attccctgcg tgcggcatcc aggggatttt
     9061 cattttcgcc aaattcccct ttcgggatgg accagcttcc ctcatccttt tttacccaaa
     9121 agggtccgcc gggatgtaca aggaaaactt ccggacgctc atccgccaaa cgaaacaata
     9181 agatgcctgc actctttttc atcctttgat aaccgcaagc ggtttcaacc gggctacttt
     9241 gatggcgagc ccggcttcgt gtaccgtatc gaccaccaga tccacgtcct tataggcaag
     9301 aggggcttcc tctgcgatgc ctccgaatga gccggcctgg atataaatgc cttccttgcg
     9361 caaagcctct ttgagttcag gaccgcctac ctgttttttt gcagccgtcc gggaaagttt
     9421 tctcccggct ccgtgacagg tggaaccaaa ggccgaatcc atgctgttcg gcgcgccggc
     9481 caggacaaag gaggaggtgc ccatgctgcc tggaatgagg accggttggc cgatggcccg
     9541 gtatttctcc ggaatatcca gatggccggg accaaaggcc cgggtggctc ccttccggtg
     9601 tacaatcagt tttgtcggga caccggccac ggggtgatct tctatcttgg cgatgttgtg
     9661 cgcgacatcg tataataatt tcacatgccc gggattcaaa ttaaataccc gctgaaggct
     9721 ttgccggatt ttatggctga tgatttcccg gttgcaccag gcaaaattgg ctgcggcggc
     9781 catggcatta aaataggact gtccttcagg agcattaaag ggaaccccgg caagttcccg
     9841 gtccctgggt ttgaaaccgt actggtccat gacatttacc atgagccgga ggtaatcggt
     9901 ggctacctgg tggcccaggc cccttgatcc ggtatggatg agtatcacga tctgatcctg
     9961 cgacaggccg aaagcggcgg ctgcctgatc attaaaaatg gcggagatcc ggtccatttc
    10021 cacaaaatgg ttacccgaac cgatggtacc gagttggtcc aggcctctgc gtttggcttg
    10081 ttccgatacc ttttccggat cggcattggc caggcaaccg ttggactcga taaactccaa
    10141 atcttccggc tcgcccagtt gatgggaaac ggcccaggct gctccctgcc tcaataccgc
    10201 gttcatttca ttcaggttga gcaggacatc gcctccttta ccggttccgg acgggacact
    10261 tgagtataaa tctttggaaa atgcttccac ccggttttca acatccctga ataccaatgg
    10321 ggtcaaaagc aggcgcacgc cgcaattgat atcgtaccct atcccccccg gtgagataaa
    10381 tccgtccggc caccgggtag cggccacacc cccaatggga aaaccatacc cttcgtgggc
    10441 atccggcatc acgatggcag cccgctgaat accgggcagg ctggctacgt tgatcagttg
    10501 ctccagggca cggtcatgcc ggatcgcgtc cagcaattct tcccggcaat aaatatgccc
    10561 cggaacccgc atggcgggat gggcggacat gggaatctcc caaaggacgg gacccagccg
    10621 gttcaatgga tattccttgc tcattggatc aattcagatg tcaaacacca ggatgctttc
    10681 ccattgtcca ttttcacttt ttttcaattc cgcctcatgg taggtgaccg ctttgatatc
    10741 cttgtcaaac tggtctgtcg ccttccccct gaggatggcc tttaattcag tttcggccag
    10801 ggtatcgaag atcacatcac aaaatatcgt tttgcgtata tgactgaggg taagcacttc
    10861 ggataaaaaa tccaccagca aactggttcc gtcaatagat ttcaattcca gtctttcttc
    10921 ccgggagaag tccggcttat cttttttaca aaatccttcc ttgatcaaat ccgccatcgc
    10981 ctgcaccgca agcccgaaca cctctcccct cgtgcttccc gtcactttca accggatgtc
    11041 cgcggtatga ggataaaacg aataaggcat aactgcaata taaaccttaa tccgaaggct
    11101 ttacaatcca aaagcataat aaaaaaggcc gggagatccg ggatatatgc cctcaatcat
    11161 cattccgcct tgtttatccc tgagttcatc caggaattca ttgcggttgt taaccgactt
    11221 tccatttacc cgcaatacga tgaatccggt tttaatcccc gtacccgaca gggcgccttt
    11281 cgtgatccgg cttattttca cgcccccctc aatgtttaga tttctccttt cctgggggga
    11341 caggttttcg atttcgatac ccaacacctt taataaggcc gcctcgctct gcctggtttc
    11401 ggtttccggc aacagggtta ccgggatgat tttttcctcg ccgttccgga tcaccctcag
    11461 ttccagtgtt tccccgggtc cgtgccttgc tatgatttcg cgcaactggg ttgacgtccc
    11521 gacactgcgg cggccggctt ctatgattac gtcatttttc tgcacccccg ccctttttgc
    11581 cgcacccagg gattgaaggc tgtcgaccaa tacgcccgtg gtctgccgga tacccagcaa
    11641 tttggctttc tccccgttca tatcgctgat aaaaacgccg aggtaagcat ggacgacctt
    11701 tccaaaccgg agcaaatcat ccatggtttt tttgacaatc tccaccggaa tggcaaagga
    11761 gtaccctgca taggttccgg tcggtgtggc tatggccgta ttgattccga ccagcctgcc
    11821 gtaaacatcc acgagggctc ccccgctgtt gcctggattg acggcggcat cggtctggat
    11881 aaaggattcg atggcagcct ggtcggagat aatattgatg ttgcgtgctt tggcactgac
    11941 aatacctgcc gtgaccgtag aagccaggtt gaaaggattg cctacggcca aaaccacatc
    12001 ccccacctcg atggaatcgg aattcccgaa ttcaataaaa ttcaggttct tttcctcaat
    12061 cttcagcagg gccaggtcgg tcgccgggtc caccccgatt gtttttgcct ggtaactgcg
    12121 ctggtcataa aggaccacct cgatgctttg cgcatcggcg accacatggt tattcgtcgc
    12181 gatatatccg tcatcgctga tgatcacacc cgacgcgctg cccatgcgca tctggggaga
    12241 attgtactcc ctccagaaat cttccccgaa aaaatccctg aaaaaatcag gcagttgcct
    12301 ttgctcgttg accgggtaca ccgatttgat atgcactacc ccgggagtgg ccctcctggc
    12361 cgcaagccgg taatggatac ccgaagccgc ggtttgcgta tagactgcgg tttcgtcccg
    12421 ccccgagttt ttctgtctct cttcaaagga agaaaaacct gcatttaatg ccggaagaat
    12481 ttcccttttt ccctggtcct tacaggcaat gagaaacagg agggataagc aaagaatagg
    12541 aaaccatttc atgggtattg gtttaaagag aaaaccccct tgatttcaat gtaaaaaacg
    12601 gaaccgataa aaaccggttc cgtcattgta tacaatttac cctgacggta aaattggtta
    12661 tgaaaaactc tctcttacaa tattaatccc ggtaaaccct taaaccaatg aacccgatca
    12721 gggataatct tgatgctgat cagttgcacc taaatggaat taaaagaatt atttaattat
    12781 ttaattcttc ctgaataact gacaaaagcg atctgtttac tgtccgccga ccaggatggg
    12841 gtattgatcg tcccttgtcc tccgtagagg taggcaatgg tcctcggcgc tccgccggcc
    12901 accggcatca gtttgaggct taccgcctta caggcagggt gcgataccgg gtcgatgtcg
    12961 acggggaagg aaatgtaggc gatccatttc ccgtcagggg aaatatgggg gaaccagtta
    13021 ttgtattcat caaaggtcag ttgctcattc ccggtaccat ccggtttcat cctccacaat
    13081 tgcatggtgc cggtctggct gctgttgtaa taaatccact ggccgtccgg agacccttcg
    13141 gggccatcga catggccgaa ggggagccgg gtcaattggg tttcggcccc cgtctcaata
    13201 ttcgctttat acacatgata gggtgagttt ctcgtggtat ccctgcggcc gacataatag
    13261 acttccttgt tgttggccga ccagccatgc aggtaggatg gcgttttttc cgtcaccagt
    13321 ttgggaatgc cgccggtgat gggcacgaca taaaccgtcg atcccccgcc gaacatcccg
    13381 gtgcgatgat ggctgatccc gaccattttt ccatcaaaag agatgacatg gtcgttgttg
    13441 ttgcggtctg caaagcccgt attgaatttc accgggcttc ctttccctga aagaagtacg
    13501 gtccaaagcg atcctttgct gttgatcaac agacttttgc catcgggcat gaaattgggc
    13561 gcttcaaaac gtacggggct ttcgtagacc acccgccttt tcccggtgaa tacgtccagg
    13621 acttccagcc tgctgccaat ggtgtccgcg cccccaaaat taaaattagg cggcatgggg
    13681 cggtcgatcc gcacattcca gacccgggct tcttccagca catcggcatt gtgggaacag
    13741 ataaatattc cgacataggg attgtccccg atgttttcca tcacctgtga accggcggac
    13801 tgcaggggct ctccccaatt ggcaatcctc atggtatagg tcttgccctt acgctccaac
    13861 tgaatcgtcc ttgccctttt tttgggaaaa aagatctcat cctgcgggtc acgcatatag
    13921 gctccgcgca tgaccctcca ctgggccacc gtcaggccat cgcctccatg ataggtggcg
    13981 ctgaggtggg cggcgtcgtc gttgaggttt tcccttacca tccagcctac tttccggtgc
    14041 gcttcctttc cggcggcagg aaattcaaaa tcggcggtca ctataaaatc gccctggatc
    14101 ctcttggtta aaaaatggaa ttcgtcgcgg ttgaaccaaa tattgtatcc cgcacctttt
    14161 atatggtatg cctgattttc agcatcgtag gcagaactgc ccgaaatttt gggtttcccg
    14221 atgtccacag ccgtgtcaaa aacaccaaac cctttggatt gtgccttggc cggcgcaatg
    14281 gcaatgaaga aaatacccag gaaaaagatc gatttaaagc gcatgttgaa tgattaagac
    14341 ggtttgaata agttgaagaa caagggctgc ccgtccacca gggatcggta cagccccgtt
    14401 aaaaataaaa aatgtatgaa taaactagtt gtaaccggga ttttgtttga gcaccatttt
    14461 acccgatgaa ttctcaagat caatctgggt ttgaggcaaa gggaaaaatt cacttgcgcc
    14521 tttggtaaac cgggcggtcg gattgacgga atggaatgat ttttttgttt tttccacagc
    14581 ctggtaggca ttcaggatat tggccattga accggtcccg ttgtcccagc gctgcagatc
    14641 aaaaaaccgg tggccctcca tggccagttc gagcctgcgt tcaaactgca ccgctttccg
    14701 ggcgtagtcc ttgcccatgg acgcaaaccg gcctgcaggg taagcgctga tcttataatt
    14761 atccgccggc accgaagcgg cggtgacggt gtatttggcg gctgttgcgc tgtaagcggc
    14821 attcttatag acccagcccg aaggatcggc ggccctgttc cggaccaggt tgacatattc
    14881 ctgtgccttg tccaggctcc cggtttccac ttcacattct gctgcccaga ggatgatgtc
    14941 cgcaaatcgg ataaaaatat aattgttggc attgatctgg gtcgcacccc agaaagcggt
    15001 ctcattggag gacaggctgc caaactggct tttggcatag acgtttttct tgggactgaa
    15061 cacgccgttg accgaaagat cccggatcca ggcgggggtg tgcgggccgt aatccaggta
    15121 aggaatgccc ggccggccaa tggtccagtc gatgcgcgga tcgagggggc cgtcgtaaac
    15181 gttggcgggg ttttcatcgt taaccccttt tccttcattc caggtatcta aaagcggaag
    15241 gccgttttca tcggttttga aagcattggc caggttgaaa gagggattgt tgaagccgca
    15301 gcatccacct ggtgccccgg atccattggg gaaatttaat acatcgccaa agtttccgtt
    15361 gagctggctg ccgtcgttga ccgacatctg gcaggcaaac acggattcgg ccccgttttt
    15421 ggtctgggcg ttgaaattgc tctcaaaatt cacaaggctg tatttttccc ccttggccgt
    15481 cactccattg gcgataaggt cttcgagcag gggttttgcc tgcgcgaatt tgtcctgaaa
    15541 catataggcc ttggccaggt atgcctgggc cgcccattta ttgactcttc cggcttgcgc
    15601 ctgggtggcc ggcaggttgt ccctggcgaa agccaggtct gcttcgatct taggccatat
    15661 attgacaaaa ttgcccgcat catcaatatt ggggacattc aggttgttat tggacaggct
    15721 tacggattca tccacatagg gaggattgtt ccacatcttc tgggcatcca tatgataatg
    15781 cgccctcaga aacctggctt ccgcaatcat gcggctggct tccgccgcat cgatatccgt
    15841 agcctggggg atcaaacgca tcacttcatt ggcccgctgg atgccggtgt acaggatcac
    15901 ccatttgtag ttcatataat tattggtacc cccggtagcg gacaggctcc aggtggccaa
    15961 ttgtacaatg ggtggttggt cggtgggggt cgaaccttta taagaatcgt cggcgcaaac
    16021 actgccataa atccaattag aacaggcaga agcataggtg atcccggtag ggttaacgac
    16081 tccggccacc ccggcaaggt tggtcaaacc cacctggccg tcaagaaggg aataggcccc
    16141 gatcagcagg gcgctgatcc cggacttatt ggtgacgttg gaggcgctga gggctccaag
    16201 aggttgttta tcgagaaaat cattgcagcc aaaattcact gcgaggagca ggccgctgag
    16261 ggtaaaaagc gaatggatat ataatcgttt catgatttcg cgttgagtag gaatgattaa
    16321 aaagttatgt taagtcctcc ggtgaacatt ttttgattgg ccgggtaagc tcccccatcg
    16381 ataccaaaat tggtgctggt cagggtggag ctcaacaatt cagggtcaag tccggtgtac
    16441 ttggtgaagg tgaacagatt gaccgcctgc acatagatcc tcagcctgct ggccccgatc
    16501 cggtcaagtt tggaaaccgg tacggaataa cccaattgca ggttttttaa tcttacaaaa
    16561 gaaccgtctt ccatcaggta agaattaaag gcggtcacat tgctgaaatt ggcgccgcgt
    16621 tccagcctgg gcaccttggc gttgggccgg tcgggcctcc aggaattgta gaccgcgtct
    16681 ttgctgatgg ccacatcaaa ttgctgggga aaatcaatgg agttcctgac ctggttgatc
    16741 acttcgttgc ctacgttggc ataccagaac atggacaaat ccaggttttt atatgtggcc
    16801 ccgagcgtca ggcctgcggt aaagtccggg ttgggattgc cgaaatgcgt ccggtcattg
    16861 gcatcgatct tattgtcccc gttgatgtcc tcaaagcggt gacggcccgg ggcggcagca
    16921 tcctgtttgg gagcggcatc cacttcagcc tggctctgga aaaggccaat ttgtttgtaa
    16981 ccgaagaaag ccccgatcgg gtgaccgggc tggaggcggg attggccata atcgatgtag
    17041 agaaaacccg gaggcacgct tttgacctcg ttggcatagg aggtgaaatt gcccccgatg
    17101 tcgaaggaaa ggtcttcacc cagcttgccg tgataggtga gcgaaagatc gaccccgcta
    17161 ttctggatat ttccggaatt tacaaaagga ggcctcgacc ctcctgccgt cgcgggaatg
    17221 gcggcgggaa acaagaggcc gctgatttct tttttatacc attcggcgga gaggtcgaac
    17281 ctgcccttta aaaggctggc atcaaaaccg atattggtga tgatgtcctc ctcccaggtg
    17341 gtattgacgt tgccaaactg gctggcaaac aatccctgga ccggtgtagt gctggtcccg
    17401 ttgatatcat aatacgattc gacgctgctt tggctgaaca gggtaaaggc attggtgggg
    17461 cttacattgc tgatggaacc gagtttgccc cagcctccac gcagtttcag atcgtccagc
    17521 caggagatgc ccttcatgaa aggctccgcc gagatcctcc atcccaccgt cacggaaggg
    17581 aaaacaccgt aacggttctc cggtgcaaag acggaggatc cgtcccggcg aagggtaccg
    17641 ctcagataat atttgttttc gaaattatag tccagcctcc caaaaaggga aaagagggca
    17701 ttttcataag gcgtctgaaa tccgttgatt gcgatattcg aattgctttg cccggtcggt
    17761 gtgccgaaat tgagcgtcca gaggtttggg tccacggtga gattggccgg attggttaca
    17821 taataggagc cccgggcccc gctgatgccc cggcccgcgt tggaaatggc ttccgagcca
    17881 atcaggacat tgaggttgtg tgaccctgca aacgtattct tataattcag ggtattggtc
    17941 caggtccggc tggtgacgct gccgtaattt tcaataaaac tgtttgggtt ttgggcattt
    18001 tccgcatttt cataagccgt gaatacaaaa ctgtaattgt tgaacaagtc caccgctcct
    18061 ccgaaactgg tcttgaccgt aaagtttttt aggaaatcca cttcggcgta tacattgccc
    18121 aggatcccgt aattattggc tttgttgtcc ttggtcctct ccataatggc caccgggttg
    18181 ggcgaattgc ccaggctgcg cgatccgccg cccgcgaaat tgcctttgat atcatatacc
    18241 gggacgatgg ccggcatgcg gaaagcggcg ttgatggaat tggcattgtt gatgccggga
    18301 aggcccaggt acccgggatt ctttttataa ttgaaataag cgctttgacc gatgcggata
    18361 cttttgctta cgttgtaggc gctgttgatc cgggcgccat accgtttcag atagttgcgg
    18421 atcagggtgc cgtcctggtc gagataattc agggaaagga ggaaggtggc cctttcactc
    18481 cccccgctga tcgacaaatc gtggttcatg accgtagccg gtttgaagat ctcatgaaac
    18541 cagtcggtcc cggccttgtt tgctttggtg atctggttgc tgtacagggc ataggtggcc
    18601 ggatcggttt ttgcactttc ccccacctgt ccggtaggcg tgatgtagtc cgggatcacc
    18661 ggggtggccc cgtttccgta ttgggcgtgg atggggggga gattgctgtt tttaaattgt
    18721 tcccagatcg cgttggcatt ttccgtggga ttcgccaggt tccagccgtc tttaagcggg
    18781 cgttgggtac cgatataggt atcgtaagac acggtgattt tgccggcctg cccccttttg
    18841 gtggtaatga tgatcacccc gttggaaccc cgaactccgt aaatggccgc cgcgcccgca
    18901 tccttcagga cctggatgga ttggatgtcg ttggggttga ggtcgtgcat cgaacccggg
    18961 gtgccgtcga tgatcaccag ggggtcggtg ctgcctacgg aggtgatccc tctgactctc
    19021 acattgctgg gactgcccgg ggcgccggaa ttgatgacgg tcacccccgc ggcattgccc
    19081 tggaggaccg attcaatggt gcccgaaggc acggctctga ggtttttcat gtccaccacg
    19141 gccaccgccc ccgtgatgtc tttctttttc tgggccgtat agcccgtgac gacgatttca
    19201 tccagcacgg tgccggctcc catcgcgata tccacggtca gcgggttggt ggaactgatg
    19261 gtcacggaca ggctgtgggt ttcgaatcct acataggata cggtcagtgt ataggtgcca
    19321 aacggaatgt cctggatcag gtattttccg gaggcgtcgg tcgagcttcc aaggcccaac
    19381 tcggtaatgc ccacatttgc gaattccagc ggttcgccgt cgccggagac ggttccctgg
    19441 agggaccccc tttgagcgta cgagaattgg caagagagcg acaagaataa aatcaaggca
    19501 acatatcgtt tcatacaaaa aaggttgaca gattaaattt tccaaagaac aatcgattgc
    19561 ataaaacaat cgattgcact gcgcgttatc ctttgcagcg gtttcaaatg tagatcaaat
    19621 ccttttttac cgcaatcgat tgcataaatt tttaaccatt tcggcacttt tgtattaaac
    19681 ttggtcaaac gaagactggt gttttaacat ctgtaccgca ttaaacccta attttggaag
    19741 acgccaattc gaataaagcg ccggacttga agaagaaaaa gacccacgtc accatccatg
    19801 acctggccgc ggaactgaag gtatccccct ctaccgtatc ccgggccctc cacggccatc
    19861 cgggcatcag ccaggaaagg atcaaagcca taacccgcct ggctaaaaag cggaattatg
    19921 tacccaatga gttggcggcg ggcctgcggc agagccggag caactgcatt ggggtgatca
    19981 ccacctggat cgaccgcaat ttccattcgt ccctgatccg cagtgtggaa gaagaggtgt
    20041 ccagcaaggg aatgaatgtc atcatcctcc agtcccacga ccggtacaag gacgaggtgg
    20101 aaaacgccaa agccctgctc aacagccggg tcagcgggct catcgtatcc ctggccatgg
    20161 aaacccggga ttacagccat ttcaagcctt tttttgaaag acagatcccc gtgatcttcg
    20221 cggaccgggt gccccgcgaa atccatgccc acaaggtcct ggtggacaat ttcaaggcgg
    20281 cctaccaggc caccagccac ctgatcgagc agggttaccg gcacattgcc cacctgtcgg
    20341 gccagcagca ccgggacaat tacctcgacc gccaggaagg gtatgtgcag gccctgaaag
    20401 accatgggat caggccggac aaaaggctga tctacgaatg cgaaaccaaa agcgccgaag
    20461 aaggggtcct ggccgtgcag caactgctct ccctcaaaca accccccgac gccctgttca
    20521 gcacgaacga cacgatggcc gtcagcatcc tgcaatactt caaaaaaata gggatccgcg
    20581 taccccggga tttcgggatt gtcggattca acaacgatcc gatcaccagc atcatcgacc
    20641 ccgccctcac caccacctac aaccctgcgt acgagatcgg aaaacacgcc gccaagttat
    20701 tgctggaaca ccagcggcaa aactggacgg aaaaggactt taaaaccatc cggttggaaa
    20761 cggaattgat tgtgagggaa tcgacggata aaaaagggtt gaggaatgag ggttgagggt
    20821 taaagggagt attcaataaa gtgtgtcagg tacttcaagg cgggctgctg agtctgaggc
    20881 cgtaggccga agacgaagca gcccgccttg gccaattttt gggatcccat cattggaggt
    20941 gtttggtata ccggttgcca aaaacttcaa taagttcacg ctggatagga gcccagttaa
    21001 acacgttttt tttccaccct ttcgtattga accttaagtt caaatatagc tgttttagca
    21061 ggctttttgg attgacccaa gccc
//
LOCUS       DAOQXT010000082        20897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2912_length_20897_cov_6.283130, whole genome shotgun sequence.
ACCESSION   DAOQXT010000082 DAOQXT010000000
VERSION     DAOQXT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20897
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_20897_cov_6.283130"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKM27_13720"
     CDS             <1..969
                     /locus_tag="PKM27_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNR08371.1"
                     /translation="DKETLGGARTHSEISGITDYRVPDDLSALNTIRQMVSRLSHPVS
                     AGFNRVEAVAPKRLPGDILGNYPLQANKPYDVRAILESIVDDGSLLPYKEDYGKTIFT
                     AYARIEGWSVGIVANNREVVKTGKGEMQFGGVIYSDSADKATRFILNCNQKKIPLVFM
                     QDVTGFMVGSRSEHGGIIKDGAKMVNAVANSTVPKFTIIAGNSYGAGNYAMCGKAYDP
                     RLIVAWPTAKIAVMGGHQAARVLADIQINKIKAGGHVPDQAEEQDLYQKIKARYEEQE
                     SPYYAAARLWVDSIIHPAKTREFIALGIEMANHAPVEKFNVGVIQV"
     gene            complement(1218..1502)
                     /locus_tag="PKM27_13725"
     CDS             complement(1218..1502)
                     /locus_tag="PKM27_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08372.1"
                     /translation="MFQRLRQKWGVSGLDLALILITFAIGGSLCGYLAKVLLKGTPLE
                     GGVLWVIMYILLVTALWPVCVVFISIFTGQYTFFKKYVGKMASRIFGGNK"
     gene            1740..2852
                     /locus_tag="PKM27_13730"
     CDS             1740..2852
                     /locus_tag="PKM27_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNR08373.1"
                     /translation="MRLLAKTFSGLEKVLEAELKKLGAGKTKPITRGVEFEGDQGFLY
                     KSNLCLRTALEILVPVYSFRFDSQAQYYSRLYEFEWSDYLDFSKTFAIHHNVYSKIFT
                     HSQYAALVCKDAIVDHLRKKHTRRPNVDAQDPDVRIDLHILENQCTISLDSSWPSLFK
                     RGYRKAAFDSPINEVLAAGLIDLSGYDGSMDFYDPFCGSGTFATEAYIKASGIHAQHW
                     RENFGFRRWFDYNDALYQKILKKVNKPIGEIPHKIFASDLELYSMDACRINWKFAKIS
                     RRIQAGMEDFFKSKPKSESGLILMNPPYDKRIPLRDTERFYKDIGYKLKDDYRGFKAW
                     IISPYTDIVEGVKLKKIKSYKVYNGSLECKFIGFEC"
     gene            complement(2927..4522)
                     /locus_tag="PKM27_13735"
     CDS             complement(2927..4522)
                     /locus_tag="PKM27_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08374.1"
                     /translation="MKAIFTFGLLVLLFGFTQAQRTVSLKLVGDNNVPLKAEEVGDIK
                     VSWYGQDGIDVIYTETFPDPSVVEGLINLELGTGANNTAYTEAVNAYFRNQVPASETS
                     IKVNVEVSLTNGKLLNFDKEINLNDPLPVIKVINDQSVVILPAQINLVDGNRDVYING
                     REMFMKEDTSDILLSHVDPRGMNIYGGANQTAQFSSDGIYVRKEEDYAKYLFGTGVEL
                     KNTTYNTFYGLSGGNVLNLSNNDHFNFSTNEFSLGRSSNKFWFFNQSGGGFNPSAGRT
                     HEFTSDFFGIKDDITKRESRLFYNGISGFHNNQVRFNLGISSSGSPLFQFLNSAGKLL
                     YNMSPNTDGVTDITNHNPDGNFTYRITSLSGTAGKNPYVYITQNGGPPGAGMYYNSNN
                     VPVLFASVKNFRMDHPDLADQDIVYASLEGPEAAAYCRGKGQLTQGKQFIPFPDHFKF
                     VCSPDDLTIQLTPRSAKSRGLAVTEITKEGFRIEELMEGAGNYEFFWEVKGTRKGFED
                     YQVIRPKSEVMPVPFKRQEMNKE"
     gene            complement(4519..4998)
                     /locus_tag="PKM27_13740"
     CDS             complement(4519..4998)
                     /locus_tag="PKM27_13740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08375.1"
                     /translation="MRAIWTILLCISLLDLRGQRIAKISFPSAAGVARQDGFTIQYII
                     GDRVAGYAALEGDARMFVGFLDPVFSSISTSVTAERMERIQVFPNPFKEQISIRHSLT
                     GAGLRYRLTDLHGRILLQGSDRKWDMTFPTAHLAPGIYILSLEQAKGHFALYKLIKI"
     gene            complement(5135..5617)
                     /locus_tag="PKM27_13745"
     CDS             complement(5135..5617)
                     /locus_tag="PKM27_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08376.1"
                     /translation="MKKSITIFFFVLSFVNMVSGQTEKGTWLLGGNASYQRSSGKGTL
                     FLSPSIGYFLVNHVAGGAQLNLIAITGSTSWNLGPFAKYYFYGDERGRFYASLGLNIG
                     DAPGSNFDTGYSLGGGYAKFLNSSISIDMGVQYLRAGSSEGIFSLGAGFQIHFNSLRN
                     "
     gene            5782..6810
                     /gene="mgrA"
                     /locus_tag="PKM27_13750"
     CDS             5782..6810
                     /gene="mgrA"
                     /locus_tag="PKM27_13750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HNR08377.1"
                     /translation="MKINDLYDPGFYRANENRYDRMQYRRCGRSGIQLPMISIGLWHN
                     FGHTDNLENGRNILRTAFDQGITHFDLANNYGPPFGAAEENFGKLFKQDWVPYRDELL
                     ISSKAGWDMWPGPYGNLGSRKYLIASCDQSLRRMGLDYVDIFYSHRRDPDTPLEETMG
                     ALDHLVRQGKALYVGISQYNAADTAKASEILKAMGTPLLIHQPRYSMMDRWVETGLLN
                     ELEKRGVGAIAFSPLEQGILTDKYLKGFPKDSRAVKDGRYLTKEKITAEVLSKVTRLN
                     ALATKREQSLAQMAVAWLLKDPRITSVLVGASSPRQLLDSIGALKNLTFTKQELRDIE
                     TILKNKPS"
     gene            6812..7135
                     /locus_tag="PKM27_13755"
     CDS             6812..7135
                     /locus_tag="PKM27_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08378.1"
                     /translation="MKIFCLLLCCFLLHPGMGYSQGLAPASVKSMDTLAVRFHFIPFY
                     QYKAAVHKTANQPNKRPFRSPYDHHDALGFFCKWELKMDKQVRQAIRFRLGSVDYVNR
                     MEGKQ"
     assembly_gap    7274..7373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7472..8191)
                     /locus_tag="PKM27_13760"
     CDS             complement(7472..8191)
                     /locus_tag="PKM27_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08379.1"
                     /translation="MTKRILKSLLLALMAGLIILPACNTEDLSADAVGDLDALTDEST
                     YALEQRGNLGKHGCYDLIFPLSIVFSDGSKVEVTSQDSLRNAIRAWHQAHDAMKKEKP
                     DFVYPIQVMAEDGSIIDVADKAALSALREACHKTYLDSVCRRDSLHHGGFPRHDTLCF
                     TLVFPVQVTKADGTVITITSLDDLKVQTRDEHKKGRGHGIKHGLTNQLQLVFPVTVKK
                     SDGSTITILSKEELKALREGC"
     gene            complement(8300..9835)
                     /locus_tag="PKM27_13765"
     CDS             complement(8300..9835)
                     /locus_tag="PKM27_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08380.1"
                     /translation="MEEKIPEDKLERFLLSSLKQYGDSPSPEVWDRIEAGLRTAPVGV
                     YRRLPWMVGTAAAGLLAVLCIAVVLLVRSNQELKAELRKVQSLVPESKMHDRNEKNTI
                     NTGTRTHNGEEHNDLKAPKEGNKADASGPVQELDTLSGSNYQKALLLEKNQMKGVAGS
                     EKRRALKKGEKISEPPAVKDQTAIAANEAPILKVDSQPGSSEEQIKQVENWAVPPQIQ
                     AGSLVRNDLGELKREAQSLMSQQVHSPISAATIPARSRISIGFLGGPLSESGTIKPIR
                     PFDPRDTEQAKSEYHAMNSWQAGLELEKVLGPSWSITGGLSYKHFEFVQEIDQKLAFR
                     NREQHHPGRPPLQHDFRYRMQGPAGAAEFEINSEQLDSRATIDDSELIQVNIVNRTAL
                     NYLSIPVTAGYHLHFGRWQAFAKAGIQSDLLIHRDAQDPEVRISHPQLALKNNKPRAN
                     SQPVNAIGFNGLVQAGVKYRQGSRWGLQLSPSLFLPLTRRDPHIRSNARVYSVQVGLT
                     YAL"
     gene            complement(9839..10369)
                     /locus_tag="PKM27_13770"
     CDS             complement(9839..10369)
                     /locus_tag="PKM27_13770"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR08381.1"
                     /translation="MEGCRRGRREDQHRLYELLKDRMFGMCLRYSHSREDAEDILQDG
                     FIAVFRDFRQYKGDGPIEAWVRRVILNTALRHLRRQTKDIFIQAENEENQASDEITEE
                     DHFEKEMMIQYLLKTIQQMPPGFRAVLNLYILEGRSHEQIAGTLGISPGTSKSQLNRA
                     KEYLRKALARSLQAKG"
     gene            complement(10462..11175)
                     /gene="bshB1"
                     /locus_tag="PKM27_13775"
     CDS             complement(10462..11175)
                     /gene="bshB1"
                     /locus_tag="PKM27_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNR08382.1"
                     /translation="MVDILAIGVHPDDIELSCSGYLLKEIHAGRKVALLDLTQGELGT
                     RGNVQIRKKEALEAAKKMGALSREFLNLGDGFFDNNKKAIEPLIVKIRKWRPSIILAN
                     ALEDRHPDHGRAAELVGRAAYLSGLRKIETRLNGKKQQVWRPQAVYHYIQDYPLKPDL
                     VVDITGFLDQKMELIQTFKSQFYDPASLEPDTPISSADFLDFQVARARVYGKYISVKY
                     AEGFMVARPVGVSSLLPLV"
     gene            complement(11177..12226)
                     /locus_tag="PKM27_13780"
     CDS             complement(11177..12226)
                     /locus_tag="PKM27_13780"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNR08383.1"
                     /translation="MLRSKVAGIGIYLPEKVVTNHDLERVMDTSDEWIQERTGIRERR
                     YAKKHEESTSTMGAIAARRAMDDAGLEPADIDFVIFATLSPDYFFPGCGVLLQRALKL
                     GDVGALDVRNQCSGFIYGLSVADQFIRSGMYKNILLVGAEFHSAGLDFTTRGRNVTVI
                     FGDGAGALVLQPAGDQDSMILSTHLHSNGAQAEALAYINPGCHGGVHLGTERFGYPNQ
                     EFGGAFLTQKMIENEDYYPNMDGQAVFKSAVVKFPEVILEALDANGFQVSDLDMLIPH
                     QANLRISQFIQKKLGLRDDQVYNNIMRYGNTTAASIPIALYEAVQEGKVKKSNLVCLA
                     SFGSGFTWGSALIKW"
     gene            complement(12299..13576)
                     /locus_tag="PKM27_13785"
     CDS             complement(12299..13576)
                     /locus_tag="PKM27_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNR08384.1"
                     /translation="MKKSSFLVLVMVLLNSLVLQASMILIPMDPNQKNHLKAYGVTYW
                     VLQQNSEAYWLLNYLGGSFAFEYRPEFEKECKTRGITYQVIPNAAFNQILTEISNPEV
                     NQDAIKLEVAPKVAVYTPEYDHEGNRIQPWDDAVMLALTYAEIPYDKIYDEAVLSDKL
                     AKYDWLHLHHEDFTGQYGRFYSGNHTAPWYRQHVRESEEMAKKLGFKKVSQLKLAVVK
                     RIHDYLLGGGFLFAMCSATDTYDIAMAADGVDICAEVFDGDPMDGDCQERLDFSKTLA
                     FRNFRIMKNPFDNEFSSIDNNGRKVNPQNDYFTLFDFSAKWDPVPTMLTQNHTRTVKG
                     FMGQTTAFVKDYIRPDVLILGENKSMNEVRYIHGTRGQGTWSFYGGHDPEDYRHFIND
                     PDTDLNLHPNSPGYRLILNNILFPAAKKKERKT"
     gene            13695..15782
                     /locus_tag="PKM27_13790"
     CDS             13695..15782
                     /locus_tag="PKM27_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08385.1"
                     /translation="MSESPALIWILCFGTGFLFWLILYFRDKTFSSIHPAWKILMYLM
                     RGLATGMLLYLILAPLFKSVVTEIKKPVIVFAQDNSQSIASNWSKEKQNRYLAEKTAL
                     EESLASRFEVLSFTFGEKVVENGPVDFSERATDIGSALDYIENQTAGQNLGAIILATD
                     GNYNQGGNPLYSHPSLAVPVYTILMGDSTLKRDLSIQRVLHNNIVYLGDRFEVQVELK
                     ALQCNGERTNLRIFRVENGQEVLLQTELINIPSGDWHHTHRFILDAAKAGTAHFVIRA
                     SVLNNEYAVENNARDLFVDILDARQKILILAASPHPDLAVIKKILEGNKNYITETVQA
                     GNFTGKWADYSMVIFHQLPSRTFDLAPVIRDLNASQKPRIFILGSQADPVRFNASQTL
                     LRIAGDGRNTNEVSAEVRESFSLFTLDPAWKEFFRNFPPVSAPFGEYSASAGAHILMN
                     QKIGNIITSYPLLMIGEDQGIRTGIFAAEGIWKWRYFNFLENKNFDILDDLFRKVVQY
                     IGIKEDKRKFRVVSPQKVYLENQRVIFDAQIFNDSYEPVNADEVFMTILSPDRKEYQF
                     TFSKTDRAYTLDAGLFAPGSYTYTAYTNTRGGRQEVKGSFIVQKIQLETYQSTADHNL
                     LRLLAAQQDGKALFPEQVASLEKELSQQNAIKPVQYSSQRTYPIINFRWIFALLALLL
                     SAEWFLRRYHGAY"
     gene            15903..16946
                     /gene="pheS"
                     /locus_tag="PKM27_13795"
     CDS             15903..16946
                     /gene="pheS"
                     /locus_tag="PKM27_13795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNR08386.1"
                     /translation="MTNTDWTSIRDRFLEAQKGMEQEVVDSPASLEQFRIKYLGTKGI
                     LKDLSGYLKEIPVENRKEAGKLLNQIKQKTESLFQQYQQTYSSAGKASKAEIDLSAPG
                     YPLPLGSRHPVSLVMNQVIDIFKRIGFTIAEDREIEDDWHNFTALNTPDDHPARDMQD
                     TFYIKDSIQYLLRTHTSPVQARVMTREKPPIRIIAPGRVYRNETVSARSHCQFHQVEG
                     LYIDEGVSFADMKQTLLYYAREQYGPEVKIRLRPSFFPFTEPSAEMDVYYGLADETAH
                     RITKGTGWLEILGCGMVDPAVLVNCGIDPDKYSGFAFGMGIERQVLLKYNVPDIRMLF
                     ENDVRFLRQFSAI"
     gene            17161..18513
                     /gene="hisS"
                     /locus_tag="PKM27_13800"
     CDS             17161..18513
                     /gene="hisS"
                     /locus_tag="PKM27_13800"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNR08387.1"
                     /translation="MTKPSLPKGTRDFLPSEVMKRKYLFGILEEVFLRHGFVPIETPS
                     LELMQTLTGKYGEEGDRLLFKILNSGNFLEEADVAALEQKDSFKLAGSIAKRGLRYDL
                     TVPFARFVVMNRDKISFPFKRYQIQPVWRADRPQKGRYQEFYQCDCDVVGSKSLLYEA
                     DLLSIYSQAFSRLGIEVAIHVNHRAILAGIAEASGIAGHFTDMTVCIDKLDKIGEAAV
                     HEEMVKRGIGPEAATRVLQLIQSTTLESLIPSLSGTASGKKGIDDLKAIFNMLKGYTL
                     QQEIRFDPALARGLSYYTGFIVEVVAKNAKMGSLGGGGRYDNLTGSFGLPDVPGVGIS
                     FGAERIFDVMESMDLFPKNIGGSLQALIVALDPESMEYGIRITQTLRSSEIAADQYPE
                     LVKMNKSMKYANDLKIPFVVIIGEEERNTGRLSLKDMVSGNQEKLEVGELIKKIKTPI
                     "
     gene            18510..19940
                     /gene="gatA"
                     /locus_tag="PKM27_13805"
     CDS             18510..19940
                     /gene="gatA"
                     /locus_tag="PKM27_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNR08388.1"
                     /translation="MTSSYRDLSQLQRSLKEGQVTVSGLVRHYLKNIESSRDLNQYIE
                     VYEEEALHAAAALDEKIKTGQPLGKLFGLILSHKDIISYKGHALTASSNILEGYTALF
                     HATVIERALAEDAILIGRVQCDEFAMGSSNENSRYGPVRNPFDKERIPGGSSGASATS
                     VASDTCWASLGTDTGGSVRQPAAFCGLVGFKPGYGRISRHGLIAYASSFDQAGFLTRS
                     VYDAALLLEVAAGYDGHDASMGQATPEQYSQALDVPEKPRLAYLDLLHFKDAMDPAIL
                     KNYHETLESWKNKGFALDPVALDWLDYVIPAYYVMTTAEASSNLSRYDGIRYGYRADD
                     ATTLEQLYKRTRTRGFGTEVKRRILLGTYVLSAGFYDAYYQKAQKVRRLVRNRLDELF
                     RQYDALVMPVSPTLPWKIGEKIDDPVSVYLADIFTVLANVAGLPGISLPTGVDARGLP
                     IGTQLVGGQWKEQKLLRIAHALTLQE"
     gene            19950..20576
                     /locus_tag="PKM27_13810"
     CDS             19950..20576
                     /locus_tag="PKM27_13810"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNR08389.1"
                     /translation="MDAHELKTRLTQALERPLPGLSAHRRMAIPGRYIDAPIPDTARK
                     AAVLILFYSKLDRAFTSLIRRQTLETDRHSGQISFPGGKMESFETSPVQTALREAREE
                     IGVDGDLEILGSLTPLYIPVSNFHVYPIIAWSEKHPSFIIQPEEVDELIELDLDLLFN
                     PDLKTAVDIPVLSSLVLEKVPVYEMHGMIIWGATAMMLSELEIILAGG"
BASE COUNT         5091 a         5468 c         5143 g         5095 t
ORIGIN      
        1 gataaagaga ccctgggagg ggccaggacc cattccgaga tttccgggat cacggactat
       61 cgcgtacccg atgacctcag cgccctcaat accataaggc aaatggtcag ccggctcagt
      121 catccggtct ccgccggctt caaccgggtg gaggccgtgg caccgaaaag gctccccgga
      181 gacatcctgg gcaactatcc cctgcaagcc aacaaaccct atgatgtccg ggctatcctg
      241 gaaagtattg tagatgacgg aagcctcctt ccctacaaag aagattatgg caaaaccatc
      301 tttaccgctt atgcgcggat cgaaggttgg agtgtgggca ttgtggccaa caaccgcgaa
      361 gtcgtgaaaa ccggaaaggg ggaaatgcag tttggcgggg tcatttattc cgacagtgcg
      421 gacaaagcca cccggtttat tctcaactgc aaccagaaaa aaatccccct ggtcttcatg
      481 caggatgtca ccgggtttat ggtgggttcg aggtcggaac acgggggcat tatcaaagac
      541 ggtgcgaaaa tggtgaatgc cgtggccaat tcgaccgtac cgaagtttac catcatagcc
      601 ggcaattcat acggggccgg caattatgcc atgtgcggca aagcttacga cccccgcctg
      661 atcgtagcct ggccgaccgc caaaatagcg gtcatgggag ggcaccaggc ggcacgggtc
      721 ctcgcagata tccagatcaa caagatcaag gccggagggc atgttcccga ccaggccgaa
      781 gagcaggatt tgtatcagaa aatcaaggcc cgttacgaag agcaggaatc cccctattat
      841 gccgctgcca ggctttgggt ggacagcatc atccatcccg cgaaaacgag ggagtttatc
      901 gccctgggaa ttgaaatggc caaccacgcg cccgtggaaa aattcaatgt cggggtcatc
      961 caggtataaa gtttctgaag ccgtggcgtg aaaaattaaa tcagcgattt cttaaaaatt
     1021 tataaaagat gatttcgcca aacaataggc accaaggatt ggtttggcgg cgtgccaaaa
     1081 aaccgtccga ggacgaagcg gccaagggtg accggaaaaa ttgttgggaa tgctgattgc
     1141 aatgctttac tcccagagcg agtatttttt ggaacgagtt ttttggttac ttttttgcgg
     1201 aaaaaaagta acaagattta tttatttcct ccaaaaatcc tggaggccat cttgcccaca
     1261 tattttttaa aaaaagtata ctgcccggta aatatgctga taaacaccac gcagaccggc
     1321 cacagggcgg tgaccagcag tatatacatg atcacccaaa gcacccctcc ttccaatgga
     1381 gtgcctttta aaaggacttt ggccaggtaa ccgcacagac tgcccccgat cgcaaaagtg
     1441 atcaggatca gcgccaggtc caggccggat acgccccatt tctgcctcag gcgttgaaac
     1501 atggggcgaa gatagggaaa atggtgcttg gcggatggtg gatggtgctt ggatagccca
     1561 tacactattc ccgaaaaagt cgtggggaac gggaggtcaa tgcctgaaat atcctgaccc
     1621 tttataaagt gtttttcacc cttaaaaaac ggtcaaccaa attcaggcat ggacaaccca
     1681 ccatccacca tccaccaccc accatccacc acccaccatt tcgttacctt tgccttccaa
     1741 tgagattgct ggccaagacc ttttccggac tagaaaaagt gctggaagca gaattaaaaa
     1801 aactgggggc cggcaaaaca aagccgatca cccggggcgt tgaatttgag ggcgaccagg
     1861 gctttttgta caaatccaac ctttgcctcc ggacggccct ggagatcctg gtcccggtat
     1921 attccttccg gttcgacagc caggcccagt attacagccg gttatatgaa tttgaatgga
     1981 gcgattacct ggatttctcc aagacatttg ccatccacca caacgtctat tccaaaatct
     2041 ttacgcacag ccagtatgcc gccctggttt gcaaggacgc catcgtcgat cacctgcgga
     2101 aaaaacatac ccgcaggcct aatgtggacg cccaggaccc ggacgtgcgc atcgacctgc
     2161 acatcctgga aaatcagtgc accatttccc tggacagttc atggccttct ttgttcaaac
     2221 ggggttatcg gaaggcggca tttgattcac ccatcaatga agtgcttgcg gcaggcctca
     2281 ttgacctgag cggatacgac ggctcgatgg atttttacga tcccttttgc ggttcaggca
     2341 cctttgccac ggaagcctac atcaaggctt cggggatcca tgcccagcac tggagggaaa
     2401 attttggttt ccggaggtgg tttgattaca acgatgcact ctaccagaaa attcttaaaa
     2461 aagtaaacaa acccattggc gagatccctc ataaaatctt cgcctcagat cttgaattgt
     2521 attccatgga cgcctgccgg atcaactgga aattcgccaa gatcagccgg cggatccagg
     2581 ccggcatgga ggattttttt aagtccaaac ccaaaagcga aagcggcctg atcctgatga
     2641 atccccctta cgacaaacgg atccccctcc gcgataccga acggttttac aaagacatcg
     2701 gctataagct aaaagacgat tacaggggtt ttaaagcctg gatcatttcc ccgtacacgg
     2761 atattgtcga aggggtgaaa ctcaaaaaaa tcaaaagcta caaggtctac aacggatcgc
     2821 tggaatgtaa gtttatcggg tttgagtgct gaggacagag gttagaggtt agaggttaga
     2881 ggttagagta gttcctacct cgcacctctt acctcttacc tcgtacctat tccttattca
     2941 tttcctgtct cttaaaaggc accggcatca cctctgattt gggccggatc acctggtaat
     3001 cttcaaatcc tttgcgggtc cctttaactt cccagaaaaa ttcataattg cccgcccctt
     3061 ccatgagttc ttctatccgg aagccttctt ttgtaatttc tgtcacggcg aggccccttg
     3121 attttgccga acgcggggtc agctggatgg ttagatcatc cggggagcag acaaatttga
     3181 agtggtccgg aaaagggata aattgtttac cctgggtgag ttggcccttc cccctgcagt
     3241 aggctgcggc ttcagggcct tccagcgatg catagacaat gtcctggtcc gcaaggtcgg
     3301 gatggtccat ccggaaattt tttacagaag caaacagtac cgggacattg ttgctgttgt
     3361 aatacatgcc agctccaggc ggtcccccat tctgggtaat ataaacgtag ggatttttcc
     3421 ccgccgttcc gctaaggctg gtaatgcggt aggtgaagtt gccatccgga ttatgattgg
     3481 tgatgtcggt cacgccgtcg gtgttcggag acatattgta aaggagttta cccgcagagt
     3541 tcaggaattg gaagagcgga gagcccgaag aagaaatacc gagattaaac cttacctggt
     3601 tgttgtgaaa tcctgaaata ccgttataaa aaagccggga ttctctcttt gtaatatcat
     3661 ccttgatccc gaagaaatca ctggtaaatt cgtgggtcct gccggcgctg ggattaaatc
     3721 ctcctccgct ttgattgaaa aaccagaatt tattggatga tcggcccaga ctgaattcgt
     3781 tggtgctgaa attgaaatgg tcgttgttcg aaaggtttaa aacattgccg ccgctgaggc
     3841 cgtaaaaggt attgtatgtg gtgttcttta attccacccc tgttccgaat aaatattttg
     3901 cataatcctc ctctttcctc acatagatcc catccgaact gaattgggct gtctggtttg
     3961 cccccccata aatgttcata cccctgggat ccacatgaga taatagaata tccgaggtat
     4021 cttccttcat gaacatttcc ctaccgttga tgtaaacatc ccggtttcca tccacaaggt
     4081 tgatctgggc tggcaggatg accacactct ggtcattgat tactttgatt acagggaggg
     4141 gatcattcag atttatttcc ttatcaaaat tgaggagttt accattcgtc agggaaacct
     4201 cgacattaac cttaatgctc gtttcggagg cggggacctg gtttctaaaa taggcattga
     4261 cagcttcagt ataggctgtg ttatttgccc ctgttccaag ttcaaggttg atcaaaccct
     4321 caacgacgga tggatccgga aaggtttcag tgtaaatgac atcaatcccg tcctggccat
     4381 accatgacac ttttatatct ccaacttcct cggctttgag cggtacatta ttatcgccca
     4441 cgagttttag ggaaacggtc cgttgtgcct gtgtgaagcc aaaaaggagg accagaagtc
     4501 cgaaagtgaa aatggccttc atattttaat gagtttataa agtgcaaaat gcccctttgc
     4561 ctgctcgagg gaaaggatat aaatgcccgg agcaaggtgg gcggtcggaa aagtcatatc
     4621 ccatttccgg tcactccctt gtaaaaggat ccgtccatgc aaatccgtta gcctgtacct
     4681 aaggccggct ccggtcaggg aatgacggat agaaatctgc tccttaaagg gattgggaaa
     4741 aacctgaata cgctccatac gctctgccgt cacggacgtc gatattgaac tgaaaaccgg
     4801 atcgagaaag cccacaaaca tgcgggcatc gccttcgagc gctgcataac ccgccacccg
     4861 gtctccgatg atgtactgga tcgtaaaccc atcctgccgg gcaacccctg ctgctgatgg
     4921 aaaggaaatc tttgctatcc tttgtcccct caaatccaat aaggaaatgc aaagcaaaat
     4981 ggtccatatt gcccgcataa aaattggggt ttatactccc aatttaataa aaaatcgaaa
     5041 gaaagtaaaa ttttaagggg ctgtcctata ggccagaagt aaaacgcggg ttccttaatt
     5101 ccttaattcc ttaattcctt aattccttaa ttagttaatt tctaaggctg ttgaaatgga
     5161 tctggaatcc ggctcccagg ctgaagatgc cttcagagga tcccgccctg agatactgca
     5221 ctcccatgtc tatggaaata ctggagttga gaaatttggc atatcctccg cctaaactgt
     5281 agccggtatc gaaattcgaa ccgggggcat cgccaatatt gaggcccagg ctcgcataaa
     5341 aacggcccct ttcatcgcca taaaagtagt atttggcaaa gggccccagg ttccacgagg
     5401 tggaaccggt aatggcaatc aggttgagct gcgctccgcc cgccacatga ttgacaagaa
     5461 aataaccgat ggatggactg agaaacaagg tacccttgcc gctggatcgc tgataggagg
     5521 cgtttccgcc cagcaaccag gtgccttttt ccgtctgccc tgataccata ttgacaaaac
     5581 ttaatacaaa aaagaaaata gttattgatt ttttcatagc ccgtcatttt cgaagcagcc
     5641 ttaaagttaa aaaaattaat tttatgcccg aaggggctgt tgctgaggta tgaccatcac
     5701 ccatccgaac cgaagttggg tatgcccgga atcgggtgaa gtacactaac ttcaggcaaa
     5761 gcatccaatg caaaaaaaca tatgaaaata aacgacctgt acgatccggg attttaccgg
     5821 gccaatgaaa accggtacga ccggatgcaa taccgccgct gcggccggag cggcatccaa
     5881 cttcctatga tttccatcgg gctctggcat aattttggcc ataccgacaa cctggaaaac
     5941 ggaagaaata ttcttcgtac ggcctttgac cagggcatca cccattttga cctcgcaaat
     6001 aattacggtc cccccttcgg cgccgctgag gaaaattttg gaaaactatt caaacaggac
     6061 tgggtgccct accgggacga gctcctgatc tccagcaaag ccggctggga tatgtggccc
     6121 gggccttatg gcaatcttgg ctccaggaaa tacctcatcg cgagctgtga ccaaagcctc
     6181 cgccgaatgg gcctggatta tgtggatatt ttttattccc accgccggga tccggatacc
     6241 ccgctggaag aaaccatggg cgccctcgac cacctggtcc gccagggaaa ggccctttat
     6301 gtgggcattt cccaatataa tgccgcggat accgccaaag cttctgaaat ccttaaagcc
     6361 atgggtactc ccctcctcat ccaccagcct cgttacagca tgatggaccg ctgggtggag
     6421 acaggactgc tcaatgaact ggaaaaaaga ggagtcggcg ccatagcctt ttccccgctg
     6481 gaacagggca tcctgacgga taaatattta aaaggtttcc caaaagattc ccgtgccgtg
     6541 aaagatggcc gttacctcac caaagaaaag atcaccgccg aggtcctgtc caaggtcacc
     6601 cggctcaatg ccctggccac taaaagggaa caatccctgg cccagatggc cgtggcctgg
     6661 ttgctgaagg atccgcgcat tacctccgtt ctggtggggg caagcagtcc ccgacagttg
     6721 ttggacagca tcggcgccct taaaaattta accttcacca aacaggagct ccgggacatt
     6781 gaaaccattt tgaaaaacaa accctcctga cttgaaaatc ttttgcctgc ttctgtgttg
     6841 tttcctgctc cacccgggga tgggctattc ccagggcctg gctccggctt cggtcaagtc
     6901 catggatacc ctggccgtcc ggttccattt tatacccttt taccagtata aggcagcagt
     6961 tcataaaaca gcgaaccaac ccaataaaag acctttccgc tcgccctacg accatcacga
     7021 tgcccttggt tttttctgta aatgggaact gaaaatggac aaacaggtcc gccaagccat
     7081 ccggttccgc ctgggctcgg tggactatgt gaacaggatg gaagggaagc agtagaactg
     7141 ttgaattggt gaattgctga attgtaaaat tgttttagga ttaccatttt gttcctagga
     7201 agaggtcggt cctggcctcc tgctgcttag tgggagcttg ggaatctctg actcttgact
     7261 cttgactctt gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgactac
     7381 tgaccaacta aacaggctcc tgcttccagg agcccgttcg ttgatgctac attgatatga
     7441 aaaaataact ctacccgatc ggtgatgata ttcaacatcc ttcccgaagg gctttgagtt
     7501 cctcctttga aaggatggtg atcgtactgc catcggattt tttgacggtc acagggaaga
     7561 ccagttgcaa ctgattggtc agtccatgct ttattccatg gcctctgcct tttttatgtt
     7621 catccctggt ctggactttc aggtcatcca gactggtgat ggtgatgacc gtcccgtccg
     7681 ctttggtgac ctggaccgga aacaccagag tgaaacaaag cgtatcatgc ctcggaaatc
     7741 cgccgtggtg gagtgaatcc cgccggcaca cggaatcgag ataagtttta tggcacgctt
     7801 cacgtagggc agacaaggcg gccttatccg ccacatcgat gatggagccg tcttccgcca
     7861 tcacttgaat gggataaaca aaatccggtt tttccttttt cattgcatca tgggcctgat
     7921 gccaggcacg gattgcattc cggagtgagt cctggctggt cacctccact ttggaaccat
     7981 cgctgaaaac gatgcttagg gggaagatca ggtcataaca tccatgcttg cccaggttgc
     8041 ccctttgttc aagtgcatag gtcgattcat cagtcaaggc atcgagatcc ccgacagcgt
     8101 cagcactcag gtcttcggta ttgcaggcgg gaagaataat aagcccggcc atgagggcca
     8161 gcaacaaact ttttaaaatc ctctttgtca ttgtttaaaa ttttaggtaa aatctctcca
     8221 aactcctttg ttttattatg tggtaccggg aggcatgaaa cgttgcatgg atgtttatta
     8281 aaagcctgtt aatttattat cacagcgcat aagtcaatcc cacctgaacg ctgtataccc
     8341 gggcattgga ccggatatgc ggatccctgc gggtcagagg cagaaacaag gagggactta
     8401 attggaggcc ccaccgtgaa ccctgccggt atttcacccc tgcctgtacc aggccattaa
     8461 atccaatggc attgacgggt tgggagttcg cccggggttt attgtttttt aaagcaagct
     8521 gcggatggga gatcctgacc tcgggatcct gcgcatcccg gtggattaaa aggtcggatt
     8581 ggatgcctgc tttcgcaaaa gcctgccatc tcccgaaatg caaatggtac ccggcagtga
     8641 ccggtatgct caggtaattc agggctgtcc ggttcacaat attgacctgg atcaattccg
     8701 aatcatcgat ggtagcccgg ctgtcgagtt gttcggagtt gatttcaaat tcggctgctc
     8761 ctgcgggtcc ctgcattcgg tatctgaagt catgctgcag cgggggccgc ccgggatggt
     8821 gttgttcccg gttccggaaa gccagctttt gatcgatctc ctggacaaat tcaaaatgtt
     8881 tataggacag cccgcctgta atgctccagg aagggcccag gactttttca agttccagtc
     8941 ctgcctgcca ggagttcatc gcatggtatt cgctttttgc ctgctcggta tccctggggt
     9001 cgaatggccg gattggtttg atcgtaccgc tttctgataa aggtccgcct aaaaatccta
     9061 tggagatccg gctgcgggcg gggatggtag ccgcagaaat gggagaatgg acctgttgag
     9121 acattaagga ttgggcttcc ctcttcagtt cgccgaggtc atttcttacc aggctgccgg
     9181 cctggatttg tggtgggacg gcccaatttt caacctgctt tatttgttcc tctgaggaac
     9241 cgggctgact atccactttc aaaatgggag cttcatttgc cgcaatggct gtctgatctt
     9301 ttacggctgg gggttcgctg attttctccc cctttttcag agccctcctt ttttcagatc
     9361 cggcaactcc cttcatttgg tttttctcta aaagaagggc tttctggtaa tttgatccgg
     9421 acaaagtatc aagttcctgc acggggccgg aagcatccgc cttgttccct tctttgggtg
     9481 ctttcaaatc attatgttcc tccccattat gggttctggt cccagtattt attgtattct
     9541 tctcgttgcg gtcatgcatt ttactctccg gtacaaggct ttgaaccttt ctgagctcgg
     9601 ctttcaattc ctggtttgac cggaccagga ggacgactgc aatgcacagg actgcaagca
     9661 gaccggcggc ggctgtcccg accatccaag gcaggcggcg gtataccccg accggtgctg
     9721 ttctcaatcc ggcctcaatc cggtcccata cttcaggcga agggctgtca ccgtattgtt
     9781 tgagggaact tagcaggaaa cgttccagtt tgtcttcagg tattttttct tccatttgct
     9841 aaccttttgc ttgaagggac cgggccaggg cctttctcag gtattccttt gcccggttca
     9901 attgtgattt tgatgtaccc gggctaatgc ccagagtgcc tgcaatctgt tcgtgggagc
     9961 gcccctcaag gatatagaga ttcaataccg ccctgaatcc cggaggcatc tgctgaatgg
    10021 tttttaaaag gtactggatc atcatctcct tttcaaaatg atcttcttcc gtgatttcat
    10081 cggatgcctg gttttcctca ttttccgcct ggataaaaat atctttagtc tgccgcctca
    10141 ggtgccgcag ggctgtattc aggatcaccc tccgtaccca ggcttctatc gggccatccc
    10201 ctttgtactg tctgaaatcc ctgaagactg cgataaaacc atcctgaagg atatcttcgg
    10261 cgtcttcccg actatgactg tacctcaggc acattccaaa catcctgtcc ttcagcaatt
    10321 catacaacct atgctgatct tcccgcctgc cccggcggca tccttctatg acttggtcgg
    10381 aaacgatata ttcaggtctt tcctcttaaa tggtaccaga cccccttttt ggttgcatgc
    10441 gggggttaaa ctttttataa attataccag gggaagcagg ctcgacaccc caaccgggcg
    10501 ggccaccata aatccttccg catatttgac cgagatgtat ttgccgtaga ccctggcccg
    10561 ggctacctga aaatcgagga aatctgcaga ggaaatgggc gtgtccggtt ccagggaggc
    10621 gggatcgtag aactgggatt taaaggtctg gatcaactcc attttctggt ccaggaaacc
    10681 ggtgatatcg acgacgaggt cgggcttcag cgggtaatcc tggatataat ggtacacggc
    10741 ctggggcctc catacctgtt gtttttttcc gttgagccgg gtttctattt tgcgcaatcc
    10801 cgataggtaa gcggcccgtc caaccaattc ggcggcccgc ccgtggtcgg ggtggcggtc
    10861 ttccagggca ttggccagga tgatggaagg cctccatttc cggatcttca cgatcagggg
    10921 ctcgatggct tttttattgt tgtcaaaaaa gccatccccg aggttgagga actctctgga
    10981 gagggctccc attttttttg ccgcttccag ggcttctttt ttgcggatct gaacgtttcc
    11041 acgggtgccc aattcgccct gggtcaggtc gagcagggcc actttgcggc cggcatgtat
    11101 ttctttgaga agataaccgc tgcaggacaa ctcgatgtca tcgggatgca ccccgattgc
    11161 caatatatct accatattac cattttataa gcgcggaacc ccaggtgaat ccactgccga
    11221 aggaggcgag gcagaccagg ttggattttt tcaccttccc ttcctggacg gcttcataaa
    11281 gagcaatcgg gatggacgct gcggtcgtat tgccatacct catgatattg ttataaacct
    11341 ggtcatcccg cagccccagt tttttctgga taaactgact gatgcgcaga ttggcctggt
    11401 gcgggatgag catatccagg tcggaaacct ggaaaccgtt ggcgtccagg gcttccagga
    11461 tgacctcggg aaatttgacc acggccgatt taaaaacggc ctgaccgtcc atgttggggt
    11521 aataatcttc attctctatc attttctggg tcaggaaggc tccaccaaat tcctggttgg
    11581 gatacccaaa ccgttccgtc ccaagatgca ctcccccgtg gcaaccggga ttaatataag
    11641 ccagggcctc tgcctgtgcg ccgttgctgt gcaggtgggt ggaaaggatc atgctgtcct
    11701 ggtcaccggc gggttgaagc accagggccc cggccccgtc cccaaaaatg acagttacgt
    11761 tcctgccgcg ggtggtaaaa tcgagcccgg cggaatggaa ttcggcaccc accagcagaa
    11821 tgtttttata catgcctgac cggataaact gatcagccac ggaaaggcca taaataaatc
    11881 cgctgcattg gttccggaca tccagcgcac ccacgtcccc cagtttcagc gctcgctgaa
    11941 gcagcactcc gcatcccggg aaaaaataat ccgggctgag ggtcgcgaag atcacaaaat
    12001 caatatcggc cggctcaagg cctgcatcgt ccatggcgcg ccgggctgca atggctccca
    12061 tcgtggaggt ggattcttcg tgttttttcg catagcggcg ttcccggatc cccgtcctct
    12121 cctggatcca ttcatcagaa gtatccatca cccgttccag gtcgtgattg gtcaccacct
    12181 tttccgggag gtaaatgccg atccctgcca ctttgctgcg taacataatc ctttgattta
    12241 ggctgaaaag gtaaaaagaa tttcgaggag tttatcctta gaacagtccg ccttcccctt
    12301 aggttttcct ttccttcttt tttgcggccg ggaagaggat attattgagg atcagccggt
    12361 aacccgggct gtttgggtga aggttgaggt cggtatccgg gtcgttgata aaatgacggt
    12421 aatcctccgg gtcgtggccc ccataaaacg accaggttcc ctggccacgg gtgccgtgaa
    12481 tgtaccgcac ctcgttcatg gatttgtttt ctcccaggat cagtacatcc gggcggatat
    12541 aatcctttac aaaggcggtg gtctgaccca tgaatccttt tacggtacgg gtatggtttt
    12601 gggtgagcat agtcggcacc gggtcccatt tcgcgctgaa atcaaaaagg gtgaaataat
    12661 cgttttgcgg atttactttc cggccattgt tgtcgatgga actgaactcg ttgtcaaaag
    12721 gattcttcat gatccggaaa ttccggaaag ccagggtttt actgaagtcg agcctttcct
    12781 ggcaatcgcc gtccatgggg tcgccgtcaa aaacctccgc acaaatatcc accccatcgg
    12841 ctgccatcgc aatatcgtaa gtgtcggtcg cgctgcacat ggcaaacaaa aaaccgccgc
    12901 caagcaggta atcatggatc ctttttacca ctgcgagttt tagctgggac acctttttaa
    12961 acccgagttt tttagccatt tcttcggact cccggacatg ctggcggtac cagggtgcgg
    13021 tatggttgcc ggaataaaac cggccatact gaccggtaaa gtcttcatgg tggaggtgaa
    13081 gccagtcgta ttttgccagt ttatcgctca gcaccgcttc atcataaatt ttatcgtagg
    13141 ggatttcggc ataggtcagg gcgagcatca cggcatcgtc ccagggctgg atgcggttgc
    13201 cttcatgatc gtattccgga gtatataccg ccaccttggg ggccacttcc agttttatgg
    13261 catcctggtt gacttcgggg tttgaaattt cagtcaggat ctgattaaaa gcggcattgg
    13321 ggatcacctg gtaagtaatg ccccgggtct tgcattcctt ttcaaattcg ggccggtatt
    13381 caaaggcaaa gctcccgccc aaatagttga ggagccagta ggcttcgctg ttttgctgca
    13441 atacccaata ggtcaccccg taagctttca ggtgattttt ttgattcggg tccatgggga
    13501 tcaggatcat tgaagcctgc aaaaccaggg aatttaacag gaccataact aaaacaagaa
    13561 aactggattt cttcatagtt tttttaaaac gtttttaatt caaattcgga agtggaccaa
    13621 acaaatatca tggatatttc tacatctttg caacaattga ttccgaccca agccatattt
    13681 tgttttcagt tccaatgagt gaatccccgg ccttaatctg gattttatgt ttcggcaccg
    13741 gtttcctgtt ctggctgatt ctctatttcc gggataagac cttttcttcc atccatccgg
    13801 cctggaaaat attgatgtac ctgatgcggg gccttgccac aggcatgttg ttgtatctga
    13861 tcctggcccc tttgttcaaa tccgtggtca ctgaaatcaa aaaaccggtg atcgtctttg
    13921 cccaggacaa ttcacaatcc atcgccagca actggtccaa agaaaaacaa aaccggtatc
    13981 tcgcagaaaa aaccgcactc gaagaatccc ttgcttcgcg gttcgaggtc ctttctttca
    14041 ccttcggaga aaaagtcgtt gaaaacggtc cggtggattt ttcagaaagg gctacggata
    14101 tcggttccgc cctggattat attgaaaacc agacggcagg ccaaaacctc ggggccatta
    14161 tcctggctac ggacggcaac tacaaccagg ggggcaatcc gctgtactcc catccttctc
    14221 tcgcagtgcc cgtctatacc atacttatgg gagattcgac cctcaagcgg gacctgtcca
    14281 tccagcgggt tttgcacaac aacatcgtgt acctcggtga ccggtttgaa gtgcaggtcg
    14341 agctcaaagc tttacaatgc aacggggaaa ggaccaacct ccggatcttc agggtggaaa
    14401 acggtcagga agtcctcctt caaacggaac tcatcaacat cccttccggc gactggcacc
    14461 atacccaccg gtttatcctg gatgctgcta aggcaggaac tgcccatttt gtcatccggg
    14521 cttcggtact gaataatgaa tatgccgtgg aaaacaatgc ccgggacctg tttgtggaca
    14581 tcctcgatgc gaggcaaaag atcctcatcc tagccgcaag cccccatccc gacctggcag
    14641 tcatcaaaaa gatcctcgaa ggcaataaaa attacatcac cgaaaccgtg caggcaggga
    14701 attttaccgg taaatgggcg gactattcca tggtcatatt tcatcagctc ccatccagga
    14761 cctttgacct ggcccccgta atccgcgacc tgaatgccag ccagaaaccc aggatattca
    14821 tactgggcag ccaggctgat ccggtgcgtt tcaatgccag ccagacctta ttgcgcatcg
    14881 ccggtgacgg aagaaatacc aatgaagtga gcgccgaagt ccgcgagtcc ttcagccttt
    14941 ttacccttga tccggcatgg aaagaatttt tccgcaactt tcctccggtg tccgcaccgt
    15001 ttggagaata cagcgcctcg gcgggcgccc atatcctgat gaatcaaaag ataggcaata
    15061 tcatcaccag ctacccgctg ctgatgatag gcgaagacca gggcatccgt accgggatct
    15121 tcgccgccga aggcatctgg aaatggcgtt atttcaactt ccttgaaaac aagaattttg
    15181 atatcctgga cgacctgttc aggaaagtgg tccagtacat cggcatcaaa gaggacaaaa
    15241 ggaagttcag ggtcgtttcc ccacaaaaag tttatctcga aaaccagcga gtgatctttg
    15301 acgcccagat tttcaacgat tcctatgaac cggtcaatgc cgatgaggtt ttcatgacca
    15361 ttctttctcc ggaccggaaa gaataccagt ttaccttcag caagacggac cgggcctata
    15421 ccctggacgc ggggctcttc gctccgggca gctataccta taccgcttat accaatacgc
    15481 gcggaggccg gcaggaagtc aagggcagtt ttattgttca gaaaatccag cttgaaacct
    15541 atcaaagcac cgccgatcac aacctgctgc gccttctggc cgcccaacag gacggcaagg
    15601 cccttttccc ggaacaagtc gccagcctcg aaaaggagtt gtcccaacaa aatgccatca
    15661 aaccggttca atacagcagc cagcgcacct atcccatcat caatttccgc tggatatttg
    15721 ccctcctcgc cctcctgctc agcgccgaat ggtttttgag gagatatcac ggagcgtatt
    15781 gatgattaaa gaattaagga ttaataatta agaattccct tcctcctttt tcctttcctc
    15841 cttttctccc tttctccctt tctccctttc tccctttctc cctttctcct tacttttacc
    15901 ccatgacaaa tacagactgg accagcatcc gggaccggtt tttggaggct caaaagggca
    15961 tggaacagga ggtggtcgat tctccggcaa gcctggaaca attcaggatc aaatacctcg
    16021 gcacgaaggg catcctgaaa gacctttcgg gttatttaaa ggaaatacct gttgaaaacc
    16081 gcaaggaagc gggcaagctc ctcaatcaga tcaaacaaaa aacggaatcc ctcttccagc
    16141 aataccaaca aacctattct tccgcgggca aagcctcaaa agcggagatc gacctgagcg
    16201 ccccgggtta tcccctcccc ctgggctcga ggcatccggt ctcgctggtg atgaaccagg
    16261 tcatcgatat tttcaaaagg atcgggttta ccattgccga agaccgcgag atcgaggacg
    16321 actggcacaa ttttaccgct ctcaatacgc ccgatgacca cccggcccgc gatatgcagg
    16381 atacctttta tatcaaagat tccatccaat acctgttgcg gacccatacc tcgccggtgc
    16441 aggcccgggt catgacccgc gaaaagccgc ccatacgcat cattgcgccg gggcgggttt
    16501 accgcaatga aacggtatcc gccaggtcgc attgccagtt tcaccaggtc gagggccttt
    16561 acatagatga aggagtttcc ttcgccgata tgaaacaaac cctgctttat tatgcccggg
    16621 aacaatatgg cccggaggta aagatcaggc tgaggccatc cttttttcca ttcaccgaac
    16681 ccagcgccga aatggatgtc tattacgggc tcgcggatga aacggcccac cggatcacca
    16741 aaggcaccgg ttggctggag atcctcggct gcggcatggt ggaccccgcc gtcctggtca
    16801 actgcggcat cgatcccgat aaatactccg gctttgcttt tggtatgggt atcgaaagac
    16861 aggtactgct caaatacaat gtaccggata tccgtatgct gtttgaaaac gatgtccgtt
    16921 ttttgaggca gttcagcgcg atctgaagcc ggttctggag ctatttcaaa ctgattccaa
    16981 tgcacggggc aatcaggccg caagtatttt ctaaatatat ttgtatttgg atgtttaaat
    17041 ataaactatg tcaaaatcct acgtatttac cagcaacata tccatagaga tgaaaaactc
    17101 attcatttat tgatattttg acatcctcag acattttgaa ttattgctca atccaggcca
    17161 atgaccaaac cctcccttcc aaaaggcacc cgtgattttt taccctcgga ggttatgaag
    17221 agaaaatacc tttttgggat cctcgaagaa gtttttctcc gccacggctt tgtgcccata
    17281 gaaactccct ccctggaact gatgcaaacc ctgaccggca aatacgggga agaaggggac
    17341 cggttgttgt ttaagatcct caacagcggc aattttttgg aggaagcgga tgtcgcagcg
    17401 cttgaacaaa aggattcctt caagctggcc ggcagcattg ccaaaagggg tttacgttat
    17461 gacctgaccg tgccctttgc gcgatttgtg gtcatgaacc gggacaaaat ctctttccct
    17521 tttaaacgat accagatcca gccggtctgg agggccgacc ggccgcagaa aggcaggtac
    17581 caggagtttt atcaatgcga ttgtgatgtg gtggggtcca aatcgctcct gtatgaggcc
    17641 gacctcttgt ccatttattc ccaggctttc agtcgcctgg gcatcgaagt cgccatccat
    17701 gtcaatcacc gggccatcct cgctgggatc gctgaagctt ccggcattgc cgggcatttt
    17761 acggacatga ccgtatgcat cgacaaactg gacaagatcg gggaggccgc cgtgcacgaa
    17821 gagatggtaa aaaggggcat tggtccggaa gcagccaccc gggtgctgca actgatccaa
    17881 tcgaccacgc tggaatcctt aatcccttcc ttgtccggaa cggcttccgg taaaaaaggc
    17941 attgacgacc tgaaggcgat ttttaacatg ctcaaaggat ataccctcca gcaggaaatc
    18001 cggtttgacc cggccctggc caggggattg agttattata ccggctttat cgtcgaagta
    18061 gtggccaaga atgccaaaat gggcagcctg ggcggcggcg gcaggtatga caaccttacc
    18121 ggttcattcg gattgcccga tgtacccggc gtgggtatct cctttggcgc tgagaggatc
    18181 ttcgatgtga tggaatccat ggaccttttc cctaaaaata tagggggcag cctccaggcc
    18241 ctgatcgttg ccctggaccc cgaatccatg gaatacggga tccgcatcac tcaaacgctt
    18301 cggagttcgg agattgccgc cgaccagtac cccgaacttg tgaaaatgaa taaatccatg
    18361 aaatatgcca atgacctcaa aattcctttt gtagtcatca tcggcgaaga agaaagaaat
    18421 accggcaggc tctccctgaa agacatggtc agcgggaacc aggaaaaact ggaagtcggg
    18481 gaactgatca aaaaaataaa aaccccaata tgacctcctc ctaccgggac ctcagccagc
    18541 ttcagcggtc gttaaaggaa ggtcaggtca ccgtcagcgg cctggtccgg cattatctaa
    18601 agaatatcga atccagccgt gacctcaacc aatacatcga agtctatgag gaggaagccc
    18661 tgcatgccgc cgcggccctg gatgaaaaaa taaaaaccgg tcagcccctg ggcaagttgt
    18721 tcggactgat cctttcccac aaagacatca tcagttataa aggccatgcc ctgactgctt
    18781 catccaatat acttgaaggg tacacggcgc tgttccatgc cactgtgatc gaaagggccc
    18841 tggcggaaga tgccatcctt atcggccgcg tgcaatgcga tgaattcgcc atgggctcat
    18901 ccaacgaaaa ttcgcgctat ggcccggtga gaaacccttt tgacaaggaa cgcattcccg
    18961 gaggttcttc cggcgccagc gccaccagtg tggccagcga tacctgctgg gcttccctgg
    19021 gcaccgatac cgggggcagt gtccggcaac cggctgcttt ctgcgggctg gtcggcttca
    19081 agcccgggta tggacgtatt tccaggcatg gtttgatcgc ctatgcttct tcctttgacc
    19141 aggccgggtt ccttacccgt tcggtttacg acgccgcctt gctcctggaa gtcgccgccg
    19201 ggtacgatgg ccacgacgca agcatgggcc aggcgacccc tgaacaatac agccaagcat
    19261 tggatgtacc tgaaaaacca aggctggctt acctggacct ccttcatttc aaggatgcca
    19321 tggatcccgc gatcctgaaa aattaccatg agaccctgga atcctggaaa aacaagggat
    19381 ttgcactgga tcctgtggca ctggactggc tggattatgt gatcccggct tattatgtca
    19441 tgaccacggc ggaagcctca tccaacctgt cgcgttatga cggcatccgg tacggatatc
    19501 gtgcggatga cgccaccacc ctggaacaac tttataagag gacgcgcacc cggggattcg
    19561 ggactgaagt caaacgccgg atcctgctgg gcacctatgt attgagcgcc ggcttttatg
    19621 acgcttatta tcagaaagcg caaaaggtca ggcgcctggt ccgcaaccgg cttgatgaat
    19681 tgttccgtca atacgatgcc ctggtgatgc cggtatctcc caccctgccc tggaaaatag
    19741 gtgaaaaaat cgatgatccg gtctcggtct acctggccga tatttttacc gtcctggcca
    19801 atgtggccgg ccttccgggg atttcccttc ccaccggcgt ggatgcgcgg ggcctcccga
    19861 tcggcaccca gcttgtgggc ggccaatgga aggaacaaaa gctgctgcgt attgcccatg
    19921 ccttaacttt gcaggagtaa cccctgcaaa tggatgccca tgaattaaaa acccggttga
    19981 cccaggccct ggaacgcccc ctgcccggat tgtcggcaca ccgacgcatg gctattcccg
    20041 gaaggtatat cgacgctccc ataccggata cggcccgtaa ggccgccgtc ctgatcctgt
    20101 tttattccaa actggaccgg gcttttacga gcctgatccg gaggcagacc ctggaaaccg
    20161 accggcacag cggccagatc agttttccgg gaggcaagat ggaatccttt gaaaccagcc
    20221 ctgtgcaaac cgccctccgg gaagcccgcg aagaaatcgg cgtcgatgga gatctggaga
    20281 tcctgggaag cctgaccccc ttgtatatcc ccgtgtccaa ttttcatgtg taccccatca
    20341 ttgcctggag cgaaaaacac ccgtccttca tcatacagcc ggaggaggtg gacgaattga
    20401 tcgaactgga cctcgatttg ttgttcaatc ccgatttgaa aacggccgtg gacatcccgg
    20461 tactgtcctc cctggtgctg gaaaaagtcc cggtgtatga aatgcacggc atgatcatct
    20521 ggggggcgac cgcaatgatg ctgagtgaac tggagatcat cctggccggc gggtgagtat
    20581 ttcccacggg tggttcccac cacagcataa aatggtcgcc cacgaagaca cgaagacctc
    20641 gaagaaaaat gagatgggat ttcctattcc tttgtgccct tctctccgct gtgccgttat
    20701 gggaaatcat cgcgggcaag ccgcgtggcg gctcatcggc tcccacgaag acacgaagcc
    20761 cacaaagaaa aatgggataa gatgacttat tcctttgtgc tcttcgcttc gctgtgactt
    20821 tgtgggaaat cttagcggga aagccgcgta gcggctcatc ggctcccacg aagacacgaa
    20881 gcccacaaag aaaaatg
//
LOCUS       DAOQXT010000083        20798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2926_length_20798_cov_6.880731, whole genome shotgun sequence.
ACCESSION   DAOQXT010000083 DAOQXT010000000
VERSION     DAOQXT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20798
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2926_length_20798_cov_6.880731"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PKM27_13815"
     CDS             complement(<1..1070)
                     /locus_tag="PKM27_13815"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNR08390.1"
                     /translation="MNEFLIPSSQPLGPVKAGHLAWRSPSNIAIVKYWGKFGRQIPAN
                     PSLSMTLEDSFTETHLFYKPAGSGEDPIRYFFNNERSPSFEDRVVHFIEGVREYFPFI
                     EKLELEFHSKNSFPHSAGLASSASSMSSIALCLVSLEQDLYKRFSDKQFFLKASFIAR
                     MASGSACRSVYPCFAAWGRSDGIPDSTDAYAIACERFDPVFATFHDDIVIVSSDEKAI
                     SSSSGHALMNEHPFAESRYAQAKANARELFQVLKNGDLKRFGEIAETEAMTIHALMMS
                     SSPGYTLLLPNTLSILHKVRVFRKETGLPVYFSLDAGPNPHILYPAHIKDSVSAFIRT
                     DIQPLAENGVIIHDHCGMGPVQL"
     gene            1199..3076
                     /gene="gnd"
                     /locus_tag="PKM27_13820"
     CDS             1199..3076
                     /gene="gnd"
                     /locus_tag="PKM27_13820"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNR08391.1"
                     /translation="MARVHIIMGVSGSGKTTIGKSLSEKLGLPFYDGDDFHPPANKTK
                     MKAGIPLSDEDRQEWLGAMNRLILEQSRQEGAVIACSALKEKYRLALSKQLESPLSWI
                     VLRGDFELIHDRMMARKDHFMPAGLLQSQFEAMEYPAYGMHLSIEAGPEKLVDAILDY
                     LEKPEFGLVGLGVMGKSLARNLASKGVRMALFNQYVPGKEEKVAEKAIAAFPELASSR
                     GFEDLHAFADSLSRPRNIFLMVPAGPPVDAVIQQLLPSLSPGDILMDGGNSHFEDTDR
                     RISMLSSYDIHYLGIGVSGGEKGALTGPSIMPGGSREAWEKLSPYLSAAAARDKHGQA
                     CCTYIGPGGSGHFVKMIHNGIEYAEMQLLAEAYWILREGMQYSPERIADLFDRWNQGD
                     AAGYLLEITIKILRFKQDGRLVLDEIADIGGSKGTGSWSLNAAAQLGVPANLIGEALF
                     ARYLSSFKEARTEAAKQGGTVSAPVQVSEDQLHEAYQLARWINHHQGLEVLKAASNQY
                     GWDLKLPELTRIWTNGCIIRSSLMETLSRALMGAGQALPAHLFLVRDHRTALKEVVVM
                     ALKAKLAIPCLSASLNYLLGLSSADSPANLIQAQRDFFGAHTYRKKEDPFGKAYHTEW
                     E"
     gene            complement(3150..4370)
                     /locus_tag="PKM27_13825"
     CDS             complement(3150..4370)
                     /locus_tag="PKM27_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNR08392.1"
                     /translation="MHGKTLTFLLLLISIACLDLLLTHCGRSDHKQKLQKQDTVLLTK
                     HWESPIPHQEIPEGLTSISAASCGTCHAEIYKEWQQSTHAVAFQDPQFQAELKKDNII
                     TCLNCHTPLQNQQEFIVRGFINGDYKTPWKEVNPHFDKSLQLESITCATCHVRDGQVI
                     GALGSDRAPHKTLKDPGFLSEKLCLGCHNVVDEASPQLVCTFETGDEWNANWAKREGK
                     NCISCHMPSTERPLFPGMSKRAGHFHHFPASGIPKFKDREAGRLESMEFTESPVHPGY
                     SPGDLLNYQLKIKNSFAGHNVPTGDPERFFLIRYQWMDASGNILKEEVHRIGEEWQWH
                     PVAKKISDNNLKPKEERVYPFTQLLDRAGTYRFRVEVTKHRMTEENARYNQILGEYPL
                     SIEVYSKQYEIAVE"
     gene            complement(4451..5467)
                     /locus_tag="PKM27_13830"
     CDS             complement(4451..5467)
                     /locus_tag="PKM27_13830"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNR08393.1"
                     /translation="MPRPSPLILLIPLIFLILLIPLIPKASAQETYGSRLGWKADDRV
                     IILHVDDVGMSFESNEGAIQALEQGVASSWSIMMPCPWVPGIFHYLKEHPETDAGLHL
                     TLTSEWKDYRWEPLSGAGAVPGLVDSEGAMHSSVRAVVSLASPEEVDTEIRAQLDRCR
                     KMGWEPTHLDSHMGTLFAHPAFLDKYIRLGLEQKIPIMLPAGHNTLMRQTNPFDEQTM
                     VFLKATGEKLWNAGLPVIDDLHNFSYDWKPAASMKTREELRRFKSQKYMESFAGLKPG
                     ITFVIMHCATASDIFPHISDSGPLRQADLEAMLDPVLKDYLQKEGIHITTWREMMERR
                     KRIH"
     gene            complement(5571..5774)
                     /locus_tag="PKM27_13835"
     CDS             complement(5571..5774)
                     /locus_tag="PKM27_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08394.1"
                     /translation="MDQKVYLIQVLETSPDLVKVKFPFLDIPVEMNYRFFRTHLEKGY
                     LKIQKEEAIPPPGFFNQQVKGAA"
     gene            complement(5797..7365)
                     /locus_tag="PKM27_13840"
     CDS             complement(5797..7365)
                     /locus_tag="PKM27_13840"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNR08395.1"
                     /translation="MISNPFHRIKSINRTFVLSGMIALSVTTATLLLFTWLHPGLGKT
                     IKIEHITGSSASPAVFTLDEKGEPLVLDFTKVAAQVMDAVVHIKSTQTAIPPSPRNPY
                     EGAPNPYEEFFKNDPFRDFFNPRFFYQQPGPRNHNQPQVRVGQGSGVIISEDGYIVTN
                     NHVVADADDLEVTLHDNRTYKATVVGTDPTTDLALIQIKEKNLPTLAFVNSDAVKVGE
                     WVLAVGNPFNLTSTVTAGIVSAKGRNINILKEQFAVESFIQTDAAINPGNSGGALVNL
                     QGGLVGINTAIASPTGAYSGYGFAVPANMVSKVVEDLIKYGVVQRGVLGVMIRTVDGN
                     LGKEKNLEVTKGAYVDSLMANSAAGEAGIKPGDVIVAIGSTEIESSPQLQEVIARHRP
                     GDKLTLKVNRYGKIKEYSVTLNNRKGNTDLVSREHKEVLSRLGAELETLDAKTAAKLD
                     IRGGVKVKELYAGALRKNSNMREGFIITKVDGQPVATVDELVKVLEQKEGGVLLEGVY
                     EDLPGEYYYALGMQ"
     gene            complement(7487..7936)
                     /locus_tag="PKM27_13845"
     CDS             complement(7487..7936)
                     /locus_tag="PKM27_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR08396.1"
                     /translation="MTLIKSNPILPGFPRLFDDFFTKDVFDWGFQNYSSTQSTLPTVN
                     VIENNDAFTVEMAAPGMEKKDFQIELNNETLTISSQKEERNELSEGDRYTRREFSYQS
                     FVRSFHLPKSVVDESKIKAKYENGILRVLIPKREEAKTLPPRQIAIA"
     gene            complement(8154..9092)
                     /locus_tag="PKM27_13850"
     CDS             complement(8154..9092)
                     /locus_tag="PKM27_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08397.1"
                     /translation="MKSPMACFTKAMYPYRLTTLCIFLLACFNQCRQAIPVSRTDSFR
                     TLATLPAKLTVTSGLAPAGEGTVYVHNDSGNSPELYEINPGDGQIIRTIRISGRRNTD
                     WEELAWDSSFLYIGDFGNNSGRRKDLAILKVSRAEIVFRDTVSAGLISFHYPEQTRFT
                     WSRAHDFDCEAMIAIGDSLYLFTKNRGDLGTDIYRLPGDPGTYAALHVGHFNTRGLVT
                     AADLLHRAKNTLVLLGYETGQRQNKTFLWIAEDIEGTHFLGGRWHRRDLTFNLQAEAI
                     LFTSDSTLLMTNEQEGGKGGRIHELRLSAAPLNPIQ"
     gene            9112..9252
                     /locus_tag="PKM27_13855"
     CDS             9112..9252
                     /locus_tag="PKM27_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08398.1"
                     /translation="MHSKAESVGSAGEKLFIYMHLCFCPLKARPAKATGGMTYDSYQQ
                     AG"
     gene            9325..10149
                     /locus_tag="PKM27_13860"
     CDS             9325..10149
                     /locus_tag="PKM27_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNR08399.1"
                     /translation="MKILVPTDFSKPASVAIRYAAEFAQHTGAELVLFHALSIEGPPH
                     AMAAMKTHQILEAMEDGAKNDLIVKMHELRKMEGPSVNVSYKLVTGHPVQDMIGHYLQ
                     HHPVDLIVMGTKGASGMKKVLMGSNSAAVIGKIDVPVITVPEHARFKPVQHLVYASDL
                     NDIGGEMETLIRFCRGFDASIHILHIHSTAAPVGLDREALKNELRTKYDYTKLTLDFR
                     ESENVEEAIEDFIADTHADMLAMFTHQPTFLERLFGKSVTRELAFHSMIPLLSFKK"
     gene            complement(10399..12531)
                     /locus_tag="PKM27_13865"
     CDS             complement(10399..12531)
                     /locus_tag="PKM27_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNR08400.1"
                     /translation="MLSPKLKFLLCCLLILSVCSACKKEKAQIAFDTWEIKGGDGGSR
                     NYSSLDQINTNNVASLQLAWTYFGRDADSLNRSQIQCSPIIVDSILYGTTPGLDLFAL
                     HAGTGKEIWRSRASGRNLPGLGVNRGVMYWTDGFQKRIFFSFGHRLFCYDAMTGASVL
                     DFGELGSIDMHDGLGEDADSLLLMANTPGIVYKNLIIMGSRVSEGPDAAPGYIRAYDA
                     LSGKLVWVFHTIPHPGEPGYETWPPDAWTKVGGANNWAGMSLDTARGIVYIPTGSAAF
                     DFYGGNRKGKNLYANCILALEAATGKLKWHYQTTHHDILDRDLPSPPVLVQVKRDGKT
                     IDAVAQATKQGYLFVLDRDTGEPLFPVEERPVPASELPGEEAWPTQPFPVKPKPYARQ
                     EFTEDLINDLDSANHDYLKKQFALLRTGGPYLPPGVKPGIVFPGYDGGAEWGGQSYDP
                     ETGIFYINSNEMPWILTMVDLTPDKTMDNAKNYARGLFLRNCAICHGADLKGDGGKVY
                     PPIDQTIKKYSVPELRALLREGRRLMPAFRHLDSLDKDAIIAFIRNEAIPMRRAATSY
                     RPEVPYSMTGYNRFTGLDGHPAVKPPWGTLNAINLNTGEYEWTVPLGEVDSLTKKGIP
                     VTGTENYGGPLNTQGGLIFIAATKDEKFRAFDKATGKVLWEYQLPAGGYATPATYQVH
                     GKQYVVIAAGGGKMGTKSGDRYLAFSLQ"
     gene            complement(12599..14446)
                     /locus_tag="PKM27_13870"
     CDS             complement(12599..14446)
                     /locus_tag="PKM27_13870"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR08401.1"
                     /translation="MLQAIRFYFFNLWYWLSLAWIARVLFVAFYWKDYPGDKWSSLRD
                     CFIYGLRLDLSFASYILSFLFLMWFIYYTSRASLFFKAYRTFGMIALAAVLLITLADV
                     AIYAHWQMKINMTALQFALHPREVMASIGSDDNLPLLAFLFIVLIFVFGRWWWWLCQK
                     LRTTLAAGWRMILSAFAGFLVISGWSVLGIRGGLQLEPINQSAVYFSEQAIYNHTAVN
                     ASWNLLNKWVNNREGDNPYHYGSGEEVQNSLNTFYGDTPSVVPLTDSGIKPNIVFIIL
                     EGFTADVIRAFGGESGLTPTLDSLVQTGLIFTRFYSNGDRTYKGLPSILNAWPTQASS
                     SVTQNPDKTGALPSFSKTLANSGYHSSFYYGGESEFANIKSYLLNTGFDKIIDIKEFP
                     SSYRGLKWGVSDHYVFERMSKDLTEAKQPFFSCILTLSSHEPYDIPVEPFLKSKAIPD
                     LFRNTVYYTDWSLARFLTLSKDQPWFKSTLFVLTADHGNAMPRDYKNNYDPGRFKIPM
                     LIFGDPLREEWKGKVVDKTGSHTDLAATLLQNLELNTEAFRYSRNLLSRDLAGRAFYT
                     FDNGFGLIDESSTLVYDHVGGRVLIKEGPVTDSLLTLGKHLLQGTFNPL"
     gene            complement(14522..15052)
                     /locus_tag="PKM27_13875"
     CDS             complement(14522..15052)
                     /locus_tag="PKM27_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08402.1"
                     /translation="MSLILERIEALLSGNDLRTIGKVREIIPLIRNQSDFDALFSFMS
                     HHDRVIRMRAADAVEKISLGHPEYLQVHVAELFDLSHSAVDKEFKWHLALLLSRPVFS
                     KNEAEFAWAILERWAIDKSNSKIVRVNALEGLYHLFLQVTSRLLPFLQLLNKLADENI
                     PSINARIRKIRRHLPT"
     gene            complement(15220..16743)
                     /locus_tag="PKM27_13880"
     CDS             complement(15220..16743)
                     /locus_tag="PKM27_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNR08403.1"
                     /translation="MNPARRHIEIMDTTLRDGEQTSGISFSAYEKLTIAQILLTEVQV
                     DRIEIASARVSEGEFEAVKKITSWAASQGYIDRVEVLTFVDGNASIDWMLKAGAKVMN
                     LLTKGSLNHLTHQLKKTPEQHFSEIGEVLKKAMEMGLACNVYLEDWSNGMRHSPEYVF
                     RFLDFLVTQPVKRILLPDTLGVLVPYETHEYIHRTVQLYPGVHFDFHGHNDYDLGTAN
                     ALEAVRAGVHGLHLTVNGMGERAGNAPLASVIAVLKDYVKDVETSVDEKSLYTTSKLV
                     ETFSGFRIPANKPIVGENVFTQTAGIHADGDKKSNLYFNALLPERFGRQRLYALGKMS
                     GKANIENNLRELGISLSEEDLKKVTQRIIELGDRKEVVTQADLPYIISDVLDSSNLEE
                     KVRIENYVLTHSKGLRPSVTLRLNYQGEEFEESAQGDGQYDAFMNAIKKVYKIKNIDL
                     PLLADYYVHIPPGGKSDALCETIITWKKNGKEFKTHGLDSDQTVSAIKATQKMLNQI"
     gene            complement(16749..17345)
                     /gene="leuD"
                     /locus_tag="PKM27_13885"
     CDS             complement(16749..17345)
                     /gene="leuD"
                     /locus_tag="PKM27_13885"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNR08404.1"
                     /translation="MAYDKFTVLRSTAVPMPIENVDTDQIIPARFLKATERKGFGDNL
                     FRDWRYHPDNSPKADFVLNNPTYSGKILVGGKNFGSGSSREHAAWAIYDYGFRCVISS
                     FFADIFKNNSMNIGLLPVTVSEAFLDKIFRAIEADPKAEFEVSLPDQTVTILASGEKE
                     SFAITGYKKHNLMNGYDDIDFLHSMETEIRDFAQKHSL"
     gene            complement(17372..18772)
                     /gene="leuC"
                     /locus_tag="PKM27_13890"
     CDS             complement(17372..18772)
                     /gene="leuC"
                     /locus_tag="PKM27_13890"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNR08405.1"
                     /translation="MSTLFDKVWDAHIVRRIEDGPDVLFIDRHFIHEVTSPVAFLGLE
                     NRGMKVMYPERTFATADHNTPTINQHLPVKDPLSANQLETLERNTAKYGISYWGLGNP
                     KNGIVHVIGPENGITQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLASQCIMQP
                     KPKKMRITVSGKLSRGVVPKDVILYIISQTTASGATGYFVEYAGEVFEDMSMEGRMTV
                     CNMSIEMGARGGIIAPDETTFSYMKGRDKAPKGKDWDEALVYWKTLKTDPGAVFDKEY
                     HFNASDIEPMITYGTNPGMGVGISHHVPLAGTVDSNKTTYDKALHYMGFEEGQSMIGK
                     KVDYVFIGSCTNGRIEDFRAFASIVKGRKKADHVTAWIVPGSHIVEQQIKAEGILDIL
                     TEAGFTLRQPGCSACLAMNDDKIPAGKYAVSTSNRNFEGRQGPGARTMLASPLVAAAA
                     AITGVVTDPRTLLNNN"
     assembly_gap    18856..18955
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19164..19556)
                     /locus_tag="PKM27_13895"
     CDS             complement(19164..19556)
                     /locus_tag="PKM27_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08406.1"
                     /translation="MKFLLVLCVVLLIPLSSCNKAELTEEASSSSLDCKATTKAVVRK
                     QSLNENGSEFFYYLAVDTNIGGSNLVFPSYLDPNYRVDGKQLEIRYNTTSLKHTFVVC
                     VAGHNFDPNNPDEQSMPVVNVCKASATL"
     gene            19805..20794
                     /locus_tag="PKM27_13900"
     CDS             19805..20794
                     /locus_tag="PKM27_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNR08407.1"
                     /translation="MNPDTPQTMAATNAHKDIPGNPSTVMSSRIMLNDRSNGKPLKVL
                     SETDWQFWVHNGYVVVKNAVPREQALRTAAFVWEFEDKKPDDPATWYGAPRAEMKMKE
                     LVGSGMVEVYNHQRLWDNRQTQRVYEAFTDIWGTDQLWVTIDRANLNFPLRPGDTFKG
                     FIHWDYDPETKPQNVQGVLALADQTDENMGGFQCIPELFRTYDTWKLSQPADRDRFKP
                     DITGFEDQLVKVRLEAGDLLIFHSMQPHGIRPNHSKDKVRIAQYISMMPAEENNEALR
                     QWRIKSWKERIAPEGYAFPGDPRGWEQNKYPVAELNELGRKLLGLDRWSKGKG"
BASE COUNT         4971 a         5019 c         5534 g         5174 t
ORIGIN      
        1 agctgcaccg gccccatccc gcaatgatcg tgaatgatca ctccgttttc agccagaggc
       61 tggatatcgg tgcgaataaa ggcacttacc gaatctttga tatgggcggg ataaagaatg
      121 tgaggattag ggcccgcatc gagtgaaaaa tatacaggca ggccggtctc tttccggaag
      181 accctcactt tatgcaggat gctcagggta ttgggcagca ataaagtata acccggactg
      241 gaactcatca tcagggcatg aatggtcatg gcttcggttt cggcgatttc cccaaacctt
      301 ttcaagtccc cgttttttaa tacctggaac aattcgcggg cattcgcttt ggcctgggca
      361 taccggcttt ccgcaaaggg atgttcattc atcagggcgt gaccggagga actgctgatg
      421 gccttttcat cgctgctgac aatgacgata tcgtcatgga aagtggcaaa caccgggtca
      481 aaccgttcac aggcgatggc ataggcgtcg gtgctgtcgg gtataccgtc cgatcgcccc
      541 caggcggcaa aacaggggta taccgagcgg caggcgctgc ctgaagccat gcgggcgata
      601 aaggaggctt tcaggaaaaa ctgcttgtca ctgaaccgtt tgtagagatc ttgttcaaga
      661 ctgaccaggc aaagggcgat ggaactcatg ctcgaggcag aagaagccag gccggccgaa
      721 tggggaaatg agtttttgga atgaaactcc agctccagct tttcaatgaa cgggaaatat
      781 tccctgaccc cttctataaa atgtacgacc cggtcttcaa aggaagggct ccgttcgttg
      841 ttgaaaaagt accggatggg atcttcccca ctgcccgcag gtttataaaa caaatgcgtt
      901 tcggtgaatg aatcctccag ggtcatgctc aggctggggt ttgcgggtat ctggcgtccg
      961 aattttcccc agtattttac gatggctatg ttggaagggc ttctccaggc caggtggccc
     1021 gctttgaccg gccccagggg ctgggaggat ggaattaaaa attcattcat aaagcgctaa
     1081 agataaggca cctgcctgaa gtggaatgcc ggaattgcgg gaggtccggg acggagtagt
     1141 ttccaatatc cggaaactgc ttttccaagt gcttatcttg ggccgaaaat caggatccgt
     1201 ggcaagggta catatcatta tgggggtttc cggttccggg aagaccacga tcggcaaaag
     1261 cttgtcggag aaattgggcc ttccttttta tgacggagat gactttcatc ccccggccaa
     1321 caaaactaaa atgaaagcgg gcatccccct cagcgatgag gaccggcagg aatggctggg
     1381 tgccatgaac cgcctgatcc tggaacaatc caggcaggag ggagccgtga ttgcctgctc
     1441 ggccctgaaa gaaaagtaca ggctcgccct ttcaaaacaa ttggaatcgc ccctttcctg
     1501 gattgttttg cgcggggatt tcgaattgat ccacgatcgc atgatggccc ggaaggatca
     1561 ttttatgcct gccggattat tgcagtccca gtttgaagcc atggaatatc cggcttacgg
     1621 aatgcacctg tctattgaag ccgggccgga aaaattggta gatgccatcc tcgattatct
     1681 tgaaaaaccg gagttcggac tggttggcct gggtgtcatg ggaaaaagcc tggcccgaaa
     1741 cctggcctcg aaaggggtcc ggatggcatt gttcaaccaa tacgtacctg gcaaggaaga
     1801 aaaagtggcg gaaaaagcca ttgcggcttt tcccgaattg gcctcctcca ggggctttga
     1861 ggaccttcat gcttttgcgg attccctgtc caggccccgg aatatatttc tcatggtacc
     1921 tgccggtccc ccggtggatg ccgtaatcca gcaactgctt ccatcccttt ccccggggga
     1981 tatcctgatg gacgggggta attcccattt tgaagatacg gaccgcagga tatccatgct
     2041 ttcatcatac gatatccatt atctgggcat cggggtttcc gggggagaaa agggggcgct
     2101 cacgggtcct tccatcatgc cgggcggttc cagggaggcc tgggaaaaac taagcccgta
     2161 tctctcggct gccgcagcga gggataagca cgggcaagcc tgctgtacct atatcggacc
     2221 ggggggatcc ggccattttg tcaagatgat ccataacggc atcgaatatg cagagatgca
     2281 attgctggct gaagcttatt ggatcctgag agaaggcatg cagtatagcc cggagcgaat
     2341 cgcggacctc tttgatcgct ggaaccaggg agatgctgcg ggttatttgc tcgaaatcac
     2401 cattaaaatc ctacgattta agcaggatgg ccggctggtc ctggatgaga ttgctgacat
     2461 aggagggagc aaaggcaccg gtagctggag cctgaatgcg gcggcgcaat tgggcgttcc
     2521 tgcaaatctc attggagaag ccctgtttgc gcgttattta tcttctttca aagaagcccg
     2581 gacggaggct gcaaaacagg ggggcacggt ttccgctccg gtgcaggtat ccgaagacca
     2641 actgcatgag gcctaccagt tggcccgatg gatcaatcac caccaaggcc ttgaggtctt
     2701 aaaagcggct tcaaatcagt atggatggga ccttaagctc ccggagctca cccgcatctg
     2761 gaccaatgga tgcatcatcc gttcatccct gatggaaacc ctttcgaggg cgcttatggg
     2821 cgcgggccag gccctgcccg ctcatctttt cctggtcagg gaccaccgga cggctttgaa
     2881 agaagtcgtt gtgatggctt taaaggcaaa actggctata ccttgtcttt cggcttcact
     2941 gaattacctg ctcggcctca gttctgccga ctcccccgca aacctgatcc aggcccagcg
     3001 tgattttttt ggcgctcata cttaccggaa aaaggaggat ccattcggga aagcttatca
     3061 tacggagtgg gagtgaagaa gaggtagttt atccttttct cccgtctcct tttctcccgt
     3121 ctcctttcct cctccctatc attggggatt tattctacag caatttcata ttgtttcgaa
     3181 tagacctcta tcgacaaagg atactccccc agaatctggt tataccgggc attttcttcc
     3241 gtcatacggt gcttggtcac ctcgacccgg aagcgataag tccctgcacg gtccagcaat
     3301 tgggtaaaag ggtagaccct ttcttctttt ggtttcaggt tgttgtccga gatctttttt
     3361 gccactgggt gccattgcca ttcttcgccg atccggtgaa cctcttcctt taatatattc
     3421 ccggaggcat ccatccattg gtaacggata agaaaaaatc tttcgggatc tccggtgggt
     3481 acgttatggc cggcaaagct gttttttatt ttcagttggt aattcaggag atcgccggga
     3541 gaataccccg gatgtacggg gctttccgtg aattccatgc tttccaacct gcccgcttcg
     3601 cggtccttga attttggtat gcctgatgcg ggaaagtgat ggaaatggcc ggcccgtttg
     3661 gacatgcccg ggaacaaggg ccgttcggtg gagggcatgt ggcaggaaat gcagttttta
     3721 ccttcccgct ttgcccagtt ggcattccat tcatccccgg tctcaaaagt gcataccagt
     3781 tgaggactgg cttcatcgac cacattgtga catcccaggc ataatttttc cgaaaggaat
     3841 ccggggtctt tgagcgtttt gtggggcgcc cgatccgaac ctagagcccc aatcacctgt
     3901 ccatcgcgga catggcaggt ggcacaggtg atgctttcca attgaaggct tttatcaaaa
     3961 tggggattga cttctttcca cggcgtttta taatctccgt tgatgaatcc cctcacaata
     4021 aactcctgct gattttgcaa aggcgtatga caattgaggc aggtgatgat gttgtctttt
     4081 ttcagttcgg cctgaaactg cgggtcctgg aaggccaccg catgggtgga ttgttgccac
     4141 tccttataga tctccgcatg gcaggtgccg caactggctg cgctgatgga ggtgagccct
     4201 tccggtattt cctgatgggg tatgggcgat tcccagtgtt tggtgaggag gacggtatcc
     4261 tgtttttgca atttctgttt gtggtctgac cggccgcaat gcgtcagcag cagatccagg
     4321 caggcaatgg atatgagcag taataaaaag gtaagggttt taccgtgcat gaggtaaagt
     4381 taaggcctgg gtccgagttg tatcttcaat cgggagcacc cttggaaagc cgtgatgaat
     4441 cctccagccg tcaatgaatc ctcttgcgcc tctccatcat ctccctccag gtggtgatat
     4501 ggatgccttc tttttgcaga taatctttca ataccgggtc cagcatggct tccaggtcgg
     4561 cctgccggag agggcccgag tcgctgatgt gcggaaaaat atcggaggcg gtagcgcagt
     4621 gcatgatcac gaaagtgatg cctggtttca gtccggcaaa ggactccatg tatttttggg
     4681 atttgaagcg gcgcagttcc tccctggttt tcattgaggc cgcaggcttc cagtcataac
     4741 tgaaattgtg caggtcgtcg atcaccggaa ggccggcatt ccagagtttt tctccggtgg
     4801 ctttcagaaa gaccatggtt tgttcatcga aaggattggt ttgtctcatc agggtattgt
     4861 gaccggcggg aagcatgatg ggtatttttt gttccaaacc cagccggatg tatttatcta
     4921 aaaatgccgg atgggcaaag agggtgccca tatgggaatc gagatgcgta ggttcccacc
     4981 ccatcttgcg gcaacggtcg agttgggccc ggatttcggt gtccacttcc tccgggcttg
     5041 caaggctgac tacggccctt acactggagt gcatggcccc ttcggagtcg accaggccgg
     5101 gtacggcccc ggcgccggac aaaggctccc accgataatc tttccattcc gaagtcaggg
     5161 tcaggtgcag gcctgcatcg gtttcaggat gttcttttaa ataatggaag atcccgggta
     5221 cccatggaca aggcatcatg atgctccaac tgctggccac tccctgttcc agggcctgga
     5281 tagcgccttc attggattca aaggacatgc ctacgtcgtc cacatgcagg atgattaccc
     5341 ggtcatcagc tttccagccc agcctggagc cataggtttc ctgggcggag gccttaggaa
     5401 ttaaaggaat taaaagaatt aaaaaaatta aagggattaa tagaattaag ggggagggtc
     5461 tgggcatggc gaattttttg attgcacaaa gtatggaata aggaggaagg gaggaaggga
     5521 gaaagggaac ctcgttcaac ttcgaacaaa tgaaaaagag gtcgcctgat tcaggcggcc
     5581 ccttttacct gttgattaaa aaagccggga ggaggaatgg cctcttcctt ctggattttc
     5641 aaatatccct tctcaagatg ggtcctgaaa aaacggtaat tcatctccac gggaatgtcc
     5701 aggaagggaa atttgacttt caccagatcc ggacttgttt cgaggacctg gataagatag
     5761 actttctggt ccatggtgat cggtttgact atactttcat tgcatgccca gggcgtaata
     5821 atattctccg ggcaggtctt cataaacccc ttccaacagg acccctccct ctttttgctc
     5881 caaaactttt accaattcat ccaccgtagc gaccggttgt ccgtccactt tggtgatgat
     5941 aaatccttcg cgcatgtttg agtttttgcg cagggccccg gcgtacaatt cttttacttt
     6001 caccccgccc cggatgtcca gctttgctgc cgttttggca tcgagggttt ccaattccgc
     6061 gcccaacctg ctcaatacct ctttatgttc tctggatacc aggtcggtat tgcctttgcg
     6121 gttgttgagg gtcaccgaat attcttttat tttgccatag cggttgactt taagggtcag
     6181 cttatccccg gggcgatggc gggcaatgac ttcctgcaat tggggtgagc tctcaatttc
     6241 cgtggagcct atggctacaa tgacgtctcc gggtttgatt cccgcctcac cggcggcact
     6301 gttggccatc aggctgtcca cataagcccc cttggttact tcaaggtttt tctcttttcc
     6361 caggttgccg tccacggtac ggatcatgac gccaaggact cctctttgaa ccactccgta
     6421 tttgatcagg tcttcgacca ctttgctgac catattggcc ggcacggcaa atccataacc
     6481 gctgtatgcc ccggtggggc tggcgatggc cgtgttgatg cctaccagtc cgccctggag
     6541 gttgaccagg gctcccccgc tgttacccgg gttgatcgcc gcatcggtct ggataaaact
     6601 ttccaccgcg aactgttctt tcaggatatt gatatttctt ccttttgcgc tgacaattcc
     6661 tgcggtcacg gtggaggtca ggttaaaggg attgcctacc gccaggaccc attctccgac
     6721 tttgaccgca tcggagttga caaaggcaag agtgggcagg tttttctcct ttatttgaat
     6781 cagggcaaga tcggtcgtgg ggtcggtccc caccacggtt gccttgtaag tccggttatc
     6841 gtgcagggtg acttcgaggt catccgcatc ggcaaccacg tgattgttgg tcacgatgta
     6901 tccgtcctca ctgatgatca ccccgctgcc ctggcctacc cgcacctggg gctgattatg
     6961 attcctcggt ccgggttgct ggtaaaagaa acgcggattg aaaaagtccc ggaaaggatc
     7021 attcttaaaa aattcctcat aaggattcgg cgcgccctcg taaggattgc gggggcttgg
     7081 gggtatggca gtctgggttg acttaatatg gactaccgca tccatcacct gggcagccac
     7141 cttggtaaaa tccagaacca ggggttcgcc cttttcatcc agggtaaata ccgcgggaga
     7201 tgctgaggat ccggtgatgt gttcaatttt aatggtttta ccaagaccgg gatggagcca
     7261 ggtaaacagc agcagggtgg ctgtggtgac ggacagggca atcatcccgg aaaggacaaa
     7321 ggttctgttt atggatttga ttcgatgaaa tggattggat atcatagctt tttaatttga
     7381 gtctttgaat gacacaagga atagattaaa tgaaactctt attattaaaa agaaatctgt
     7441 aaagaacagg aaaaagggct ggccttccgg ccagccgcaa attttttcag gcgatggcaa
     7501 tctggcgcgg aggcagcgtt ttggcttctt cacgcttggg aatcagaacc ctcagaatac
     7561 cgttctcgta cttcgccttg attttggatt cgtccaccac ggacttgggc aggtggaatg
     7621 aacggacaaa agactgataa ctgaactccc ggcgggtata ccggtcgccc tcactgagtt
     7681 cgttgcgttc ctctttctgg gacgaaatgg tcagggtctc gttgttcagt tcgatctgaa
     7741 aatccttttt ctccatgccc ggagcagcca tctccacagt aaaggcgtca ttgttttcaa
     7801 tgacgtttac ggtgggcagg gtcgattggg tggaggaata gttctggaat ccccagtcaa
     7861 aaacgtcttt ggtaaaaaaa tcatcaaaga gcctgggaaa tcccggaagg attggattgc
     7921 ttttgatcag cgtcataatt accagatttt aaaggttaaa aaataaatta aaacaattaa
     7981 taaatggctt ttataaaacc gattgtgcct tagcacatga atattcccgc aattgaaatg
     8041 ccaatccttt ttctctgaaa atttgtcagt tttattaaaa aatttttaac aaagaattga
     8101 aaaattgaca aggctttgcc cggacgatgg ttaagttgac aagttaggac cgttcactgg
     8161 atagggttaa gaggggctgc gctgaggcgc aattcatgga ttctgccacc cttccctcct
     8221 tcctgttcat tggtcatcag cagggtggag tcggaggtaa atagaatggc ctcagcctgg
     8281 agattaaagg tcaggtccct tcgatgccaa cgtcctccaa gaaaatgggt accctcaata
     8341 tcctctgcga tccagagaaa ggtcttgttt tgcctttgac ctgtttcata gcccagcagg
     8401 accagggtat tttttgccct gtgcagcagg tcggccgcgg tgaccaggcc ccgggtatta
     8461 aaatgtccta catgcagggc cgcataagtc cctggatccc cgggcaagcg gtaaatatcc
     8521 gttcccaggt ctccgcggtt tttagtaaac aggtaaagag agtcgcctat ggcgatcatg
     8581 gcttcgcagt caaaatcgtg tgccctgctc catgtaaatc gggtttgttc agggtagtga
     8641 aatgaaatca ggccggcgga cacggtatcg cggaaaacga tctcggccct gctgacttta
     8701 agaatggcaa ggtcttttcg ccttcctgaa ttgttgccaa aatcaccgat atacaaaaag
     8761 ctgctgtccc aggccaattc ttcccagtcg gtatttcgcc tgccgctgat ccggatggtg
     8821 cgaatgattt ggccgtctcc cggattgatc tcgtaaagtt ctggagagtt tccgctgtca
     8881 ttgtgaacat atacggtccc ttctcctgct ggcgccagcc cggacgttac ggtgagctta
     8941 gccggaagcg tggccagggt ccggaaagaa tcagtccggg agaccggaat ggcctggcgg
     9001 cattgattga agcaagccaa taaaaaaata cacagggtag tcagccggta tgggtacatg
     9061 gctttggtaa aacaggccat cggggatttc ataaatcaag atattaaatc catgcattcc
     9121 aaggcggagt ccgtgggaag tgccggtgaa aagttgttta tttatatgca tctttgtttt
     9181 tgtcctttaa aggccagacc tgcaaaagcc accggcggca tgacctatga ttcctatcag
     9241 caagccgggt gatcttttgc accgaatacc ttcaaacaga atgctttatt tgaaaatcat
     9301 aattgataag taaccctgtt gatcatgaaa attcttgttc ccactgattt ttcaaaaccg
     9361 gcttccgtgg ccatccgtta tgccgcagag tttgcccagc atacgggggc tgagctggtt
     9421 ctgtttcatg ccttgtctat tgaaggaccc ccgcatgcta tggcagccat gaaaacccat
     9481 caaatcctcg aagccatgga ggacggggca aaaaatgacc tgattgtgaa aatgcatgaa
     9541 ctgaggaaaa tggaagggcc ttcggtgaac gtaagctata agctggttac gggacatccg
     9601 gtccaggaca tgatcgggca ttaccttcag catcatcccg tggacctcat tgtgatggga
     9661 accaaaggcg ccagtggaat gaaaaaggtg ctgatgggaa gcaactcggc ggccgtcatc
     9721 gggaaaattg atgttcccgt catcaccgtt cccgaacatg cccgatttaa accggttcag
     9781 caccttgttt atgcttccga tctgaacgat atcggaggcg aaatggaaac cctgatccgg
     9841 ttttgccggg gatttgacgc ttcgatccat atcctgcata ttcattcgac ggctgcaccc
     9901 gtgggcctgg acagggaggc gctgaaaaat gaactcagga caaagtatga ttacaccaag
     9961 ttgaccctgg atttccggga gagcgagaat gtagaagagg ccatcgaaga ctttatcgca
    10021 gatacgcatg cggacatgct ggccatgttt acccatcagc cgactttttt ggaaaggctt
    10081 tttggaaaaa gcgttacccg ggaattagct tttcacagca tgatccctct gctttctttt
    10141 aaaaaataag gcaagtccgc cgggctctct gattttgggt tttgatctac ctaaatttgc
    10201 ccgctgcata ttcggggcaa cggttttggg caaataaatt tgcccgctat ctatctatct
    10261 atctatctat cttgcgataa cggtgttggg gcaaataaat ttgtccgcta tttaccttgg
    10321 gaataagggt gtggggcaaa tgaatttgcc cgctatttat cttgggaata acggtgttgg
    10381 ggcaaataaa tttgcccgct actggagcga aaaagccaga taccggtctc ccgactttgt
    10441 tcccatttta ccccctccgg ccgcaatcac cacatattgt ttgccgtgga cctgataagt
    10501 agccggcgtc gcgtatcccc ctgcaggtaa ttggtattcc cagagcacct tgccggtcgc
    10561 cttgtcgaaa gcacggaatt tttcatcctt ggtcgccgca atgaaaataa ggcccccctg
    10621 agtgttgaga ggacctccat aattttcggt ccccgtcacc ggtatccctt ttttcgtgag
    10681 ggaatcgact tctccgagcg gaaccgtcca ctcatattca cccgtattaa gattgattgc
    10741 attgagggtg ccccagggtg gctttaccgc ggggtgtccg tccaggccgg taaaccggtt
    10801 gtaaccggtc atgctgtagg gcacttcggg acggtaagag gtggctgccc ttcgcatggg
    10861 aatggcttcg ttgcggataa aggcaatgat cgcatccttg tcgagggaat ccaaatgccg
    10921 gaaggcgggc atgaggcggc ggccttcgcg cagcaaagcc cttaattccg gaaccgaata
    10981 ttttttgatg gtttgatcga tcggtggata gaccttgcct ccgtcccctt tcaggtcggc
    11041 gccatggcaa atggcacaat ttcttaaaaa caagccccgg gcatagtttt tggcattgtc
    11101 catcgttttg tccggggtca ggtcaaccat ggtgaggatc cagggcattt cattactgtt
    11161 gatataaaat atcccggttt ccggatcgta ggactgtccg ccccattccg cccctccgtc
    11221 ataacctggg aacacgatac cgggtttcac cccgggaggc aggtagggtc cgccggtcct
    11281 caataaggca aattgttttt tgagataatc gtgatttgcc gaatccaggt cgttgatcag
    11341 gtcttctgta aattcctggc gggcgtaggg tttgggtttg acaggaaagg gttgggtggg
    11401 ccaagcttct tcacccggca attccgaggc gggcacaggc ctttcttcaa caggaaaaag
    11461 gggctctccg gtatcccggt ccagcacaaa taaatatccc tgtttggtag cctgggcgac
    11521 ggcatcgatg gtctttccgt cccttttgac ctggaccaat accggcgggg aaggaaggtc
    11581 acggtccagg atatcgtgat gggtggtctg ataatgccat ttgagtttgc cggtagcggc
    11641 ctccagggcc aggatgcagt tggcgtacag gtttttccct ttgcggtttc cgccataaaa
    11701 atcaaaagct gccgagccgg tagggatata aacgatgccc cgggccgtat ccagggacat
    11761 ccccgcccag ttattcgccc cgcccacttt cgtccaggcg tcaggcggcc aggtttcata
    11821 acccggttcc cccggatggg gtatggtatg aaagacccag accagtttac cggagagggc
    11881 atcataggcc cggatataac cgggagcggc atcgggccct tcagaaaccc tggaacccat
    11941 gatgatcagg tttttataaa caatgcccgg agtattggcc atcaacagga ggctgtcggc
    12001 atcttctccc agaccatcgt gcatatcgat actgcccaat tccccaaaat cgagtacgga
    12061 ggcgccggtc atggcatcgt aacaaaacag gcggtggcca aaggaaaaaa agatcctttt
    12121 ttgaaatcca tccgtccagt acatcacccc acggtttacc ccgagacccg gcagattgcg
    12181 gcccgaggcc ctgcttctcc agatttcctt acctgttcca gcatgtagag caaacaggtc
    12241 cagcccgggc gtggtcccgt ataaaatgga atcgacgatg atcgggctgc attggatctg
    12301 gctccggttc agggaatcgg catccctccc gaaataggtc caggccaact ggagggaggc
    12361 cacattgttg gtattgatct gatccaggga tgaataattg cggctgcccc catccccgcc
    12421 tttaatttcc caggtatcaa aggcaatttg ggccttttct tttttgcagg ccgagcatac
    12481 agaaaggatc agcaggcagc agagcagaaa tttcagttta ggggaaagca ttgatgaaat
    12541 taagaattaa agaattaaag aattaagatt tgagcattga attggtggta aggtatgatt
    12601 ataagggatt aaatgtgccc tgcagcaaat gttttcctaa agtcaataag gagtccgtca
    12661 caggcccttc ctttatcaga acccggcctc caacatgatc ataaaccagc gtggaggact
    12721 cgtcgatcag gccaaagcca ttgtcaaaag tataaaaagc ccgtcccgca agatccctgg
    12781 aaagcaaatt ccgggaatac cggaaagcct ctgtattcaa ttcaaggttt tgcaaaagcg
    12841 tggcggcaag gtcggtatgg gaacccgtct tatccaccac ttttcctttc cattcctccc
    12901 taagggggtc gccgaatatc aacatcggga ttttaaaccg gcccgggtcg taattatttt
    12961 tataatccct gggcatggcg tttccatgat cggcggtcag cacaaagagc gtggacttaa
    13021 accagggctg gtctttggat aaagtgagga agcgggcgag gctccaatcg gtataataga
    13081 ccgtgttccg gaacaggtcc gggatggctt tactcttcag gaagggttcg accgggatat
    13141 cgtagggctc atggctgctc agggtgagga tgcaactgaa aaaaggttgt tttgcctcgg
    13201 tcagatcttt gctcatccgt tcaaaaacat aatgatcgga caccccccat tttaacccac
    13261 ggtaagaaga tgggaattcc ttgatgtcga tgattttgtc aaaaccggta ttgagcaggt
    13321 aggatttgat atttgcaaat tcggattccc ctccgtaata aaagctggaa tgatatcccg
    13381 agtttgccag cgttttggag aaagaaggaa gggccccggt cttatccggg ttctgggtga
    13441 ctgaagagct ggcctgagtc ggccaggcat tgaggatgga aggcaggcct ttgtaggtcc
    13501 ggtccccgtt tgaataaaaa cgggtaaaga tcaggccggt ttggaccagg ctatccaggg
    13561 tgggggtgag gccggattcc cctccaaaag cccggatcac gtctgcagta aatccttcga
    13621 gaatgataaa aacgatattc ggtttgatcc cggaatcggt caaggggacc actgatgggg
    13681 tatcgccata aaaggtattc aagctgtttt ggacttcttc cccggagcca tagtgatagg
    13741 ggttgtcgcc ttcccggttg ttgacccatt tgttcagcag gttccaggac gcatttaccg
    13801 cggtatggtt gtatatggcc tgttccgaga aatagaccgc gctttgattg atgggttcca
    13861 gttgaagtcc accgcgtatg cccagaacgg accatccgct aatgaccaga aaacccgcaa
    13921 aagcggaaag gatcatgcgc catcctgccg ccaaggtggt ccggagcttt tggcaaagcc
    13981 accaccacca ccgtccaaaa acgaagatca ggacgataaa caggaaagcg agcaggggca
    14041 ggttgtcatc gctgccaata ctggccatga cctcccgggg atggagggca aactgaaggg
    14101 cggtcatatt gattttcatt tgccaatgag cgtagatggc gacatccgca agggtgatca
    14161 acaggacagc ggccagggca atcatcccaa atgtccggta cgctttgaaa aacagggagg
    14221 cgcggctggt ataatagata aaccacatca ggaataaaaa ggagagaata taagaggcaa
    14281 aggaaaggtc tagcctcagg ccataaataa aacaatccct gagacttgac catttgtccc
    14341 cgggataatc cttccagtaa aaggccacaa acaaaacccg ggcaatccag gccagggata
    14401 accaatacca cagattaaaa aaataaaagc ggattgcctg aagcatgagg ggcaaaaata
    14461 aggaggaagg gaggaaggcg aaaggtaaag gaggaaaggc gaagggagaa ggaggaatgg
    14521 gttaagttgg cagatgtctt ctgatcttcc ggatccgcgc attgatggaa ggaatgtttt
    14581 catcagccag cttgttcagc aattgcagaa atgggagcaa cctggaggtt acctgaagga
    14641 aaagatggta taagccctcc agggcattga cccgaacgat cttcgagttc gatttatcga
    14701 ttgcccaacg ctccaggata gcccaggcaa actccgcttc gttctttgag aatacgggcc
    14761 gggacaagag gagggcgagg tgccatttaa attctttgtc cacggcggaa tggctgagat
    14821 caaacaattc agctacatgg acctgaaggt attccggatg ccccaggctt atcttttcga
    14881 ccgcatcggc agcccgcatc cggatgaccc ggtcgtgatg ggacataaat gagaacaatg
    14941 catcgaaatc cgactgattc ctgatcagtg gaatgatttc acggaccttg ccaatggtgc
    15001 gaaggtcatt gccggacaat aaggcttcga tccgttccaa aatcaaggac atcctttaac
    15061 gattaagaac tgctaaactt acaaacttgc aaacttagta aagccttctt ccgcacgccc
    15121 cttgatccct taaaggggga gctgccgcgg attaccatca gttaactttc caacttgcta
    15181 acttggtaac ttgcaaactg actactgacc cactgaccac tatatctgat tcagcatctt
    15241 ctgggtcgcc ttgatcgccg aaaccgtctg atcgctgtcc aatccgtggg tcttgaattc
    15301 ctttccgttt tttttccagg tgatgatggt ttcacaaagg gcgtcggatt taccgcccgg
    15361 gggaatgtgt acgtaataat ccgccagcag gggcaggtcg atgtttttga ttttatagac
    15421 tttcttgatg gcgttcatga aagcgtcgta ttggccatcc ccctgggcgg actcttcaaa
    15481 ttcctcgcct tggtaattca ggcgcagggt cacagaaggc cttagtccct tggaatgggt
    15541 cagtacataa ttttcaatgc ggactttctc ctccaggttg ctgctatcca gtacatccga
    15601 gatgatatag ggcagatcgg cctgggtgac cacttctttg cggtcgccga gttcaatgat
    15661 gcgctgggtg actttcttta ggtcttcctc ggaaagggaa atgcccaatt cgcgaaggtt
    15721 gttttcaata ttggccttgc cgctcatttt gcccagggcg tataggcgct gcctgccaaa
    15781 acgttccggc aacagggcat tgaaataaag gttgcttttc ttatcgccgt ctgcatggat
    15841 gcctgccgtc tgggtgaaga cattttcccc aacgatgggt ttattggccg ggatccggaa
    15901 gccggaaaag gtttccacca gcttgctcgt ggtatacagg gatttttcgt ccacggaggt
    15961 ttccacgtct ttcacataat ctttcaggac cgcgatcacg ctggccaggg gggcgttgcc
    16021 tgcccgctcg cccatgccgt tgacggtgag atgcagtcca tgcacgcctg cccgaacggc
    16081 ttcaagggca ttggcggtac ccaggtcata atcgttgtgg ccgtgaaaat caaaatgcac
    16141 tccgggataa agctgcacgg tacggtgtat gtattcatgg gtctcataag ggaccagtac
    16201 cccgagggta tcaggcaaaa gaatcctttt taccggttga gtgaccagaa aatcaaggaa
    16261 acggaataca tattccgggg aatgccgcat gccgttgctc cagtcttcca ggtaaacgtt
    16321 acatgccagg cccatttcca tggccttttt cagcacttcc ccgatctcac tgaagtgctg
    16381 ctccggtgtt tttttaagct ggtgggtgag gtggttcagg gatcccttgg taagcaggtt
    16441 catcactttc gccccggctt tgagcatcca gtcgatagag gcatttccgt ccacaaaagt
    16501 gaggacctcc acccggtcga tataaccctg gctggcggcc catgaggtga tcttttttac
    16561 cgcttcaaat tctccttcgg atacccgtgc ggaggcgatt tcaatgcgat ccacctgtac
    16621 ctccgtgagc aggatctggg cgatggtgag tttttcataa gcggagaagg aaatcccgct
    16681 ggtttgttca ccatccctca gggtggtgtc catgatttca atatgccttc tggcgggatt
    16741 catccggttc agagcgagtg cttttgggcg aaatccctga tctccgtttc catggaatga
    16801 aggaaatcga tatcatcgta tccgttcatc aggttgtgtt ttttatatcc ggtgatggca
    16861 aaggattcct tttcgccgga cgcaaggatg gtgacggttt ggtcaggcag gctgacttca
    16921 aattcggctt tggggtccgc ctcgatggct ctgaatattt tatccagaaa cgcttctgaa
    16981 accgtgacgg gcaacaagcc gatattcatg gaattgttct taaaaatatc cgcaaaaaag
    17041 ctggagatca cgcaacggaa accataatcg taaatggccc aggcggcatg ttcccggctg
    17101 cttccgctgc cgaaattttt tcctcccacc aggatcttgc cggaataggt tggattgttc
    17161 agcacaaaat cggctttggg gctgttgtcg gggtgatatc tccagtccct gaacaggttg
    17221 tctccgaaac cttttcgttc ggtggctttg agaaaacggg ccggaatgat ctggtcggta
    17281 tcgacgttct cgatgggcat gggaaccgcg gtgctgcgaa ggacggtgaa tttatcgtaa
    17341 gccatttttt gttattgtga cgggtctggg ttcaattatt gttgagcaag gttctcggat
    17401 cggtgacgac cccggtgatg gcggctgcgg ctgcaaccag gggactggcc agcatggtcc
    17461 tcgcgccggg gccctggcgg ccttcgaaat tccggttgct cgtgctgacc gcatatttgc
    17521 cggccggaat tttatcgtca ttcatagcca ggcaggctga acacccgggt tgacgaaggg
    17581 taaaaccggc ttccgtaagg atatccagga tcccttccgc tttaatttgt tgttctacga
    17641 tatgggaacc gggcacgatc caggcggtga catgatccgc ttttttcctg cccttcacaa
    17701 tcgaagcgaa agccctgaaa tcttcgattc taccgttcgt acagctgccg ataaacacat
    17761 aatccacttt tttgccgatc atggactggc cttcttcaaa acccatataa tgcagggctt
    17821 tgtcataggt ggttttattg ctgtccaccg tcccggccag gggtacgtgg tggctgatcc
    17881 ccacacccat acccgggtta gtgccataag tgatcatggg ttcaatatcg gaggcgttga
    17941 aatggtattc tttgtcaaaa acagcaccgg gatcggtttt aagcgttttc caataaacca
    18001 gggcctcatc ccagtccttt cctttgggcg ccttgtccct tcctttcata taactgaagg
    18061 tggtttcgtc cggagcaatg atgcctcccc gggctcccat ttcgatgctc atattgcaga
    18121 cggtcatgcg gccttccata ctcatatcct caaagacttc cccagcgtat tccacaaaat
    18181 aaccggtggc tccgctggct gtggtctggg agatgatgta caggatgaca tccttaggca
    18241 ccacacccct gctgagcttg ccgctgaccg taatcctcat ttttttgggt ttgggttgca
    18301 tgatgcactg ggaagccagg accatttcga cttccgaagt cccaatacca aaggcaatgg
    18361 cgccgaaagc cccatgcgtg gaggtatggg aatctccgca gacaatcgtc atgccgggct
    18421 gggtgatccc gttttcgggc ccgatgacat ggacgatccc gtttttagga tttcccaggc
    18481 cccagtacga gatgccgtat ttggcggtat tcctttccag ggtttcaagc tgattggcgg
    18541 agagggggtc ctttaccgga aggtgctggt tgatggtggg ggtattgtgg tcggccgtgg
    18601 cgaaagtgcg ctcgggatac atgaccttca tgccccggtt ttccaggccc aggaaagcaa
    18661 ccgggctggt cacttcgtgt ataaaatgac ggtctatgaa caaaacgtcc ggaccgtctt
    18721 caatacgcct gacgatatgg gcatcccata ctttatcgaa taatgttgac atataagctt
    18781 gagatttcag gtacaaaaat ggtcaaaatc ggcttatcgg gatagtcaaa aggaggggga
    18841 actacggaag ccaagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaaa
    18961 ggtgaatggc gaaaggtaaa gggtgaaggg agcgggaaga gggcgaacgg aaaaaggcga
    19021 agggcgaagg gagcgaaaag agggagaagg gttaaagggt gaagggtgaa gggagcaaaa
    19081 attctgagat attaaaataa cgtttcagat caattgtcca tcaagccccc cggggccagc
    19141 aagaggagtc ttgcaggaaa atcttaaagc gtggcggatg ccttacaaac attgaccacc
    19201 ggcatggatt gttcgtccgg attattcggg tcaaaattgt gcccggcgac gcaaactacg
    19261 aaagtatgct tgagagaagt ggtattgtag cggatctcca gttgtttgcc atccacccgg
    19321 tagtttgggt ccaggtaaga ggggaaaacc aaattacttc cgccgatatt ggtgtccaca
    19381 gccaggtaat aaaagaattc tgagccgttt tcattgagac tttgttttct cactacggct
    19441 ttggtagtgg ctttgcagtc caggctgctg gaggaggctt cctcggttag ttcggcttta
    19501 ttgcaggagc tcagagggat taatagtacg acacaaagaa ccaagaggaa tttcataacg
    19561 agagagtttg aaccaaatat aacaaaaaag aatctgaaaa tcaaggatat ttcttttaag
    19621 tacatggatt aaagccggat ttcctgcaga gaatactctt attttatccc agagtgataa
    19681 atttcataaa ttgttttaaa taacttctat tttcatataa ttacatttcc atgacccggc
    19741 cggtgaacgg atgccctgta ttggccgccc tgtcattaca tttgtttaaa ataaataaga
    19801 tcttatgaat ccggatactc cgcaaaccat ggccgcgaca aatgcccaca aggatattcc
    19861 cggcaacccc tccacggtga tgagcagccg gatcatgctc aacgaccgct ccaatggcaa
    19921 accgctcaag gtactcagtg aaaccgactg gcagttctgg gttcataatg gctacgtggt
    19981 ggtcaaaaat gcggtgccca gggaacaagc cctgaggacg gcggcttttg tctgggaatt
    20041 tgaggataaa aaaccggatg atcctgccac ctggtacggt gcccccaggg cggaaatgaa
    20101 gatgaaagaa ctggtgggca gcgggatggt tgaagtgtac aaccatcaga gattgtggga
    20161 caaccgccag acgcaaaggg tttatgaggc ctttacggat atctggggta cggatcaatt
    20221 atgggtgacc atcgaccgcg ccaatctgaa ttttcccctt cgtccggggg atacttttaa
    20281 ggggtttatc cactgggatt acgatcccga aaccaaaccg caaaacgtcc agggagtgct
    20341 ggcccttgcg gatcagacgg atgaaaacat gggcggattc cagtgcatcc cggaactgtt
    20401 ccgcacctac gatacctgga aactcagtca gcccgccgac cgggaccgct tcaaaccgga
    20461 tatcaccggc tttgaagacc agttggtaaa agtcagactc gaggccggcg acctgctaat
    20521 ttttcatagt atgcaacccc acggcatccg gcccaatcac agcaaagaca aggtgcgcat
    20581 cgcccaatac atttcgatga tgcccgccga agaaaacaac gaagccctcc gccagtggcg
    20641 catcaaatcc tggaaggaaa ggatcgcccc cgagggttat gcctttcccg gagatcccag
    20701 gggctgggaa caaaataaat atccggttgc cgaattgaat gagttgggga ggaaattgtt
    20761 gggattggac agatggagta aaggcaaagg ttaaggtt
//
LOCUS       DAOQXT010000084        20738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2937_length_20738_cov_6.198134, whole genome shotgun sequence.
ACCESSION   DAOQXT010000084 DAOQXT010000000
VERSION     DAOQXT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20738
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2937_length_20738_cov_6.198134"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1124)
                     /locus_tag="PKM27_13905"
     CDS             complement(174..1124)
                     /locus_tag="PKM27_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNR08408.1"
                     /translation="MERKVLVIGDQIVELGEKKILRLLVGHLPSGTRIYIHIHVNRAM
                     DPGPVILLIGGMHGDEINGIEIVRQAGRKYMQSALQRGSLITIPLLNVYGFIHFTREV
                     TDGKDVNRSFPGSTKGSLASRIARMLYKKVLPQVDVLLDFHTGGNQRYNYPQIRFTKN
                     DGPSIALAEVFRAPFTLASTAKPNSLRQYASKMKIPNLAFEGGESGRFDGFSIEKGLQ
                     GIRSILEHYQMIVPAVDPALPQSHTLTGDTWIRATYSGIFIWQRASGVYVNKGEILGS
                     ISDPDNMRIEYIKSKTSGYIIGHNNAPVVHVGDALFHIGW"
     gene            complement(1124..2002)
                     /locus_tag="PKM27_13910"
     CDS             complement(1124..2002)
                     /locus_tag="PKM27_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HNR08409.1"
                     /translation="MVLAILSRNPGLYSTQSLAIAGRKRNHRMLVLDPYHCSQHIIGG
                     KPRLYYASRPLMRIDAIIPRIGATATDIGASVIDHFTSMGVYSTTTSDALLKARNKWS
                     CSQFLAARGLPVPEACLHFNAEYNEKVLQKFKYPLIIKLLKSTQGMGVLKANHKAQAL
                     NILEAFQRLEEQVIIQEFIQEVNGSDIRIIVIDGRVAGAMKRQAKKGEFRSNLHRGAR
                     AQPVRLTPEEESIAIRATRLLGLDVAGVDLLRSYRGPLILEVNASPGLEGIEQFLKSS
                     ISEKIIELVESRKPMA"
     gene            2087..3388
                     /locus_tag="PKM27_13915"
     CDS             2087..3388
                     /locus_tag="PKM27_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR08410.1"
                     /translation="MLPLVEKNNPRVVRSWAMFDWANSSYALVISAAIFPAYFLSVTN
                     ASISVGGNAVPNSSYYSFIISIAYIFVAAGSPILSGMADYSGKRLFFLRLFTWLGSLS
                     CISLFFFKGMETLWLGTAGFMLASIGFTSSLVFYNSYLPVIATEEHYDRTSAKGFAYG
                     YTGSVLLLIFNLVVIQKKEWFGIEDSGFAVRLAFITVGCWWLLFAQIPFNRLPSDSKE
                     KFGKGIWQKGYFELLAVWHKLKKQVHLRRFLLAFFFYSAGVQTVLYLAATFADTVLNF
                     GGSELIVIILILQLVGLVGAYLFAGVSRMKGNKFALLTMLFMWIAVCIAAYYTYTKFQ
                     FYLIASGVGLVMGGIQALSRSTYSKLLDENETDLTSYFSFYDVLEKLAIVIGTFSFGY
                     IDLISGGMRNSVLILIVYFAIGIVLLTRVKMSEARRDVRKQ"
     gene            complement(3653..4765)
                     /locus_tag="PKM27_13920"
     CDS             complement(3653..4765)
                     /locus_tag="PKM27_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08411.1"
                     /translation="MIRSIYSLLLFLFLLPAYASDGEFTKNYHKEYDVPKDATVELIN
                     KYGYVKVHTWEKRIASVDVNIIANTNSKSEADELFEKIQIQFTTTSNYIKAETVIQST
                     NKNWWDIRNWVGGWNKNSSYKINYDVYLPADLYLKLNHKYGDAFVDRLDRNAEVKISY
                     GNLRMDGLNADLDLELAYSDGNLGSIKRGHLNLRYSDITLAEMEDGSLNFRYSEIDLN
                     KAGTIKAESAYGELNAEYIASLDLQSRYDDIRVRSVGDIYVSSAYTDFDLGKVGKRTS
                     FSSTYGDIIIRDLDADFQSLDLKTSYTDVRISTGGAPYRLDASGSYADIKTPSDFNST
                     YQVEKSNSKSVKGYSRAENTGGLIKATLSYGGLRVD"
     gene            complement(4746..5318)
                     /locus_tag="PKM27_13925"
     CDS             complement(4746..5318)
                     /locus_tag="PKM27_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08412.1"
                     /translation="MSEDKLKRFIEQHRAEFETEKPPRQVWHRIEAELHQGGKVKSLW
                     GIIPAIVKIAASGLILLSLGIGVGYQIQRQKDFTYAIQNKDTRQEFREAEAYYTSQID
                     DKLTELGKYKSSASVLDDLNQIDQISAELKLELLKNPDQDQSILVKQMMQQYNQKLNI
                     LNKILSKLQQKENENPSLPNNKPEHDTLDL"
     gene            complement(5344..5898)
                     /locus_tag="PKM27_13930"
     CDS             complement(5344..5898)
                     /locus_tag="PKM27_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR08413.1"
                     /translation="MSTPDREDRPEERALVEDCLAGKRSAQSALYERYAQAMYQICLR
                     MLNAPEEAEDLLQEAFVDVFSHLQSYRFESTLGAWIKRIVVNRCINKLKSRKLQLVEL
                     SNHEPVLPQGHETDFEEQELKINRIKSAMKALPDGYRSVMSLYLFEGYDHQEIGEILG
                     ISEETSKSQYSRARVRLKELLSIQ"
     gene            complement(5956..6153)
                     /locus_tag="PKM27_13935"
     CDS             complement(5956..6153)
                     /locus_tag="PKM27_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08414.1"
                     /translation="MKKKRGSKVHKDIRGFDIQINQFGELKSNLNIEDLNRFLDRHVS
                     DKKIRDQAPGPEGPGTRDEEE"
     gene            complement(6197..7705)
                     /gene="gltX"
                     /locus_tag="PKM27_13940"
     CDS             complement(6197..7705)
                     /gene="gltX"
                     /locus_tag="PKM27_13940"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNR08415.1"
                     /translation="MSVRVRFAPSPTGALHIGGVRTALYNYLFAKKNHGIFILRIEDT
                     DQARYVPGAEEYILDALRWCGIEPTEGQGYGGKLGPYRQSERKAVYREAALQLVESGK
                     AYYAFDTEEELDRMRARLADAGVAAPKYDASVRMEMRNSLSLDPLEVTDLLRAGTPHV
                     VRLRVDPGKQVVYRDLIREEVRFDTTELDDKVLIKADGMPTYHLANVVDDHHMDISHV
                     IRGEEWLSSTAHHLLLYEAFEWSPPRYAHLPLIMRPDGKGKLSKRDGARFGIPVFPLS
                     WKGDTAEESFTGFREFGFEPEAVLNFLAFLGWNPGTDQELFSLDELVEAFDLGRVHRS
                     GARFDMDKALWFNHQYIKQRPVQEIVSKLKPLVEARGWLWDERKCLEIVPLFTERVNL
                     VSEIPDAAGYLFSELRTMDEEAVRKHWKPELGPVISELVKILEAVEPFEAQALEQAAK
                     AGFQSLNVKPGQILPILRLALAGGLQGPGVFQMLQILGKQGLSRIRTFVARD"
     gene            7895..8554
                     /locus_tag="PKM27_13945"
     CDS             7895..8554
                     /locus_tag="PKM27_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNR08416.1"
                     /translation="MYLIKTPKVIQNFFPNFIWQVPTREKALFLTFDDGPIPEVTPWV
                     LDELEKYQAKATFFTVGNNVRQHPDIFNRILDQGHSVGNHTYNHINGWTSENIPYFHD
                     VRHCAKLVHSSLFRPPFGRLKPGQIQFLQRHYRIIMWDVLSGDFDQEITREQCLRNVL
                     DNAGEGSIVVFHDSLKAKRNLCFVLPKVLDYFSRLGYTFECLSPHSIHQGIFEKKRIT
                     V"
     gene            8725..9978
                     /locus_tag="PKM27_13950"
     CDS             8725..9978
                     /locus_tag="PKM27_13950"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNR08417.1"
                     /translation="MTDGGSIQAVNQLRQLERELLLKQMQIQRLLNITQAINSNMSAD
                     DLFNMYREFLRFEMGMSRILLLIQEVDHWKLAVEFNLEKKYTAEALVPILRNITNLYT
                     VKATDEEILHEFDIVIPVYHKEHPISYALVTVANKEDLYHKIQFIQTITNIVAVAIEN
                     KRLFKNQMEQERMNKEIELAREVQSLLIPQTFPRSKNFELSSIYKPHFNVGGDYFDYI
                     RIGEDRYLICMADIAGKGVSAALLMSNLQGNLRQLARQYTDPEQLVIQLNEAVFALTG
                     GDRFLTLFIAEVQPARGRIDYINCGHISPILYSADRVEYLSKGTTVLGAFPKLPFIEH
                     GLIRVEDPTILLVFTDGLTDLVNASHQYFEIENLEKFVRDFHGESAEAFNQRLMIDLE
                     KFKGNQTFPDDITVLTCKIVPNAVS"
     gene            9994..10803
                     /locus_tag="PKM27_13955"
     CDS             9994..10803
                     /locus_tag="PKM27_13955"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HNR08418.1"
                     /translation="MKNRIVAGLLAILLGSVGAHKFYLRKPGAGIFYIFLFMIFLNAR
                     LPIMAILGLIEGLGLLMMSDQEFNRRYNAGRMVGQEGYPDQYPGPREIRSRERMRDRS
                     ERRGGGWKNVEVPEYPPNRRNVFKETGMQKYKEFDLEGAIEDFQKSLQMEPRDIATHF
                     NIACAFSLTEQPAKAYHHLSKAVELGFNDYEKINTHDDLAFVRIQKQWDGFKQSGYRQ
                     YAAFENQPFAEEKPEPQNDLLLDQLNKLNELRQKGLITEAEYVMEKRRLNE"
     gene            10864..11520
                     /locus_tag="PKM27_13960"
     CDS             10864..11520
                     /locus_tag="PKM27_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR08419.1"
                     /translation="MLAHDLIAEGIPAIDPHRVGEEALRRMEEYEVMHLPVVGNGQFM
                     GLLSEEEILKNGVDTPVETYLDEMSKVSVHRNDHLFDVMKKMTDSDITAVPVTDEEGH
                     YLGLITRKDLFDFYGRSFAWSEPGSILVLDLEDVHYSLSEISRIIEAEQGMIISSIIS
                     RPSETRILLTLKINQLDITRIIAALRRYDYEVTASYTEAEVADHFKERYDAFMRYLDV
                     "
     gene            11513..12922
                     /locus_tag="PKM27_13965"
     CDS             11513..12922
                     /locus_tag="PKM27_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNR08420.1"
                     /translation="MSDYRTMFRMTIGVLFLAAFCQSQAQYLYIDSIRFEGNKKTQNY
                     ILRRELDFRVHDTIAQQYLSTRLLENRSRLLNTGLFKEVHLNISRWDTETNHVTMEVR
                     VEEAWYIYPVPVFELADRNFNVWWNEFNGSLKRVNYGLRFIHNNLTGQQDGLKATLQL
                     GYSPKLEMIYSWPFFGRERAWRFRSELLYSQNRESYYKTEQDRLVFHRSESDFALQRL
                     RFLSALEHRTTLQLFQSISLEYQFNRTNDLIRNVLNPDFFLDNKARQNYLTLRYRFIY
                     DNRDLRFYPSRGLSAEFQVAKEGLAGAEDVNTLNATIQLDQYFPWTTRHNAGIMLKAK
                     GSIIRDKIPYYNSKALGYGENYIKGYEYYVMDGMDYLFLKFRERYIFFDRAMAFRKNK
                     KIGVKSMPVKLSTALHASTAYVNNVHYSFNNPLANRWLYSMGASLECLLANTSLIQLD
                     FSINHLKDTGIYLHFRDQF"
     gene            12925..13800
                     /locus_tag="PKM27_13970"
     CDS             12925..13800
                     /locus_tag="PKM27_13970"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNR08421.1"
                     /translation="MRILLFALETREETRKPLMALTDALLDQPGFEIYRYGEPVAENE
                     TRYNELPLIQSQQELLEHRIDIIITAGGDGTMLKAAAFSGATGIPLLGYNLGRMGFLA
                     VVEKDSIPMMISSLMERQYQIEERITLEVTSDPVIFPEGTFALNDFTILKRDNSSMIK
                     IHAAINGEFLNTYWADGIILATPTGSTGYSLSCGGPIIFPQSGNFVLTPIAPHNLNVR
                     PIVISDDSVLEFRVEGRTDSFLCTLDSQYALINSEYKIRLRKNSFAIRLVRLSGHTFL
                     KTLRDKLMWGLDMRN"
     gene            14128..16566
                     /locus_tag="PKM27_13975"
     CDS             14128..16566
                     /locus_tag="PKM27_13975"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR08422.1"
                     /translation="MKSLTTIVFAAVLYLTCTSSALAQDMITGKVADSKSVAYEFATV
                     LLLNHTDSSLAKGAITDPEGSFEFENAKPGKYLFKISYVGKPDILTQAFDYTGGMLQT
                     GVHAFPESATELKTVTVSAVKPVLEIKAEKMILNVENNVSSAGSDGLEILRKAPGVVI
                     DNNENITLKGKNGVQVYIDGKPARLGGRDLAALLKSYNANNIEAIEVIMNPSAKYEAE
                     GNAGIINIRLKRNQSLGTNGTAALNFRMGFTPKGNASLNLNHRKEKFNFYTNLTTAMG
                     INHENVDFYREQGGLFFDQVNRIKDDSVRFPRFNYRFGVDYLINAKHTLGAQINGNVY
                     DGVMNSHGRTRIGTLNNFGIIDSILNANTRTLETRNNFQTNLNYAYKDTMGRNFNLDA
                     DYGRINSKNDSYNPNMYMSPAEDATLSSSIFGSYTNTDITIKTLKGDYEQNLAKGRLG
                     IGFKLSQVTTGNVFDFFDYIQGVKTQDLEKSSDFNYDEMVYAAYVNYNKQIKKWAVQA
                     GLRVENTKSHGQLISLNPKDEDDVKRSYVDAFPSFAITYNASKKHTFGLTYSRRIDRP
                     RYHNLNPFEQRLDELTFRKGNPFLQPQYTQNLNFNHTLMGMLTTTLTYSRTNDFFGQI
                     IDSTQRRRTFITEKNIAQMDNYSIGIATPIPLAKWYNGYISVNAFRQIYEADFGTGKK
                     LNIATNGFNGYSEHTFTLNSGLKMQLSGWYNHGGVWGGTFVGKSMGSLDLGLQKTFLK
                     NKVDVKLSFTDVLKTAGWDAISDFGGIYFVGKGYNESQTFRVNATYRFGNMKVKGARQ
                     RKTGLEDEQRRAGN"
     gene            16777..17361
                     /locus_tag="PKM27_13980"
     CDS             16777..17361
                     /locus_tag="PKM27_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR08423.1"
                     /translation="MQFPDIHVQDKGHCLILGKSGTGKTTLLHLLAGLLPLQTGEIKI
                     DDQWVKSLGASQLDRFRGKKIGIVFQKSIFIEAISVMENLAWAQHLAGNPVDHGRILL
                     LLKRLGVEHQAHRLPYRLSIGEQQRVSIARALVNKPSVILADEPTSALDDQNADAVIR
                     LLEEQADLESAALILVTHDARIKSHFEQQIILAA"
     gene            17358..18662
                     /locus_tag="PKM27_13985"
     CDS             17358..18662
                     /locus_tag="PKM27_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNR08424.1"
                     /translation="MKLLKLSFQNLLHKPLHLTLSLVLFALGAGLIALMVTLNKQITQ
                     KFESNLAGIDLIIGAKGSPLQLVLNGLYHLDAPTGNISLQDAKPFLNPNHPLIKKAVP
                     LSLGDSYRGFRIVGTNRDFPELYKAGLKEGVWWDKPMEVVLGSIVASTAKLKLGDTFI
                     SSHGLTTDDFNQHEHAPFKVAGILNETGTVCDQLVLTSAESIWEVHEHQAKPALVEEG
                     EDKQDPVATEETHDHEEGEAHDHEHENQADSATLASREITTLLIQFKARNVQTLNLQR
                     NINQNTQLQAATPAIEINRLFSLMGIGFDAIRALAWMIALVSGLSIFISLYNSLKERR
                     QELALLRVLGATPAKLFALIIFEGLIISILGYLAGILIAHLVMHFSSDYLFQLYKYRF
                     DAWQFIREEAVLLLGSLAIGAAAAVIPAIQAYRTDIAKTLSD"
     gene            complement(18734..19825)
                     /locus_tag="PKM27_13990"
     CDS             complement(18734..19825)
                     /locus_tag="PKM27_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08425.1"
                     /translation="MKRRNFIKTAGMAAGSMTMAPGLMPNILIPRNKEKLGVALVGLG
                     YYSTDILAPALQLTQHCMLNGIVTGTPSKAETWKAKYKLADKNIYDYTRFDEIANNPD
                     IDVIYIVLPPSMHEEYVIRAAKAGKQVWCEKPMAVTAAECQAMIDACKKNKVSLSIGY
                     RMHHEPNTREIMQYTRTLPYGPIKDAYAEAGYRDGRTTHWKQIKKMGGGCMLDMGVYP
                     LNALRYSTGKEPLAVLSAKASTTRPEIYHEVEETMEFTLEFPGGVTGKGLASFGKGMN
                     SLQVNGEKGWYKLEPFQAYNGVKGMTSDGKTLNKTINNQQAKQMDDDAHSIINKKPML
                     VPGEEGLKDIRIVEAIKLAAKEGKSVSLR"
     gene            19914..>20738
                     /gene="mutY"
                     /locus_tag="PKM27_13995"
     CDS             19914..>20738
                     /gene="mutY"
                     /locus_tag="PKM27_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNR08426.1"
                     /translation="MYSWTGEHPREMPWKGERDPYKVWLSEIMLQQTRVEQGMPYFNR
                     FIRRFPDVQALAKASIKQVLKQWEGLGYYSRARNLHETARWIVRSQAGRFPEEYSSIL
                     RMKGVGPYTAAAIASFAFGLPHAVLDGNVHRVLSRYFGIDQTMDHRAGKKLFEDLADR
                     LLDKEQPARFNQAIMDFGALCCTPKNPDCTSCYLRNQCFAFKLDRIGQLPPAKKSKPR
                     RIRHFHYFIVADPGGQVVLSRRPAGDVWPGLYEFPKVETDGPQAPKVRPAGVDLPPR"
BASE COUNT         4933 a         5343 c         5284 g         5178 t
ORIGIN      
        1 gaccacgaca gtgaaaagaa aaaggagaaa ttttgaatgg cgcatgagag aaggccaaaa
       61 gtacaaacag aatggaatat gaggagtgag ggttgagggg tgaggagtga ggggtgaggg
      121 ttgggggttg agggttgagg attgagggtt gagggttgag gattgaggga ggatcaccag
      181 ccgatatgaa aaagggcgtc ccctacatgg accaccgggg cattgttgtg cccgatgatg
      241 tatccggagg ttttggattt aatgtattca atccgcatat tgtccggatc ggatatcgat
      301 cccaggatct cccccttgtt gacatagacc ccggaagcgc gctgccagat gaaaatcccg
      361 ctataggtag cccttatcca ggtatccccc gtaagggtat ggctctgagg aagggcagga
      421 tccacggcag gaacgatcat ttggtaatgt tccaggatgg acctgatgcc ctgcagtcct
      481 ttttcaatgc taaaaccgtc gaagcgcccg gattcacctc cttcaaaagc caggttgggg
      541 atcttcatct tcgaggcata ctgcctgagg gaattgggct ttgcggtgga agccagggta
      601 aagggcgccc ggaatacctc ggccagggca atgctgggcc catcattttt ggtaaaccgg
      661 atttgagggt agttgtatcg ttgattgcct cccgtgtgaa aatccagcag gacgtccacc
      721 tggggaagta cctttttata cagcatccgg gcgatccggg aagcaaggga gcctttggtg
      781 ctgccgggaa aactgcggtt aacgtctttt ccatcggtga cttcgcgggt gaaatgaata
      841 aaaccgtata cattcagcag ggggatggtg atcaggcttc cccgttgaag ggcggattgc
      901 atgtatttgc gcccggcttg ccggacaatt tcaatgccat tgatttcatc cccgtgcatg
      961 ccgccaatca acaggatgac cggacccggg tccatcgccc tgttgacatg aatatgaata
     1021 tagatccgcg taccggacgg cagatggcct acgagcagac gcaggatttt tttttccccg
     1081 agctccacga tctggtcacc gatcaccagg acttttcttt ccatcaggcc atcggttttc
     1141 gggattccac cagttcaatg attttctccg agatgctgga cttcaggaac tgttcgatgc
     1201 cttccagccc cggtgaggcg ttgacctcaa ggatgagggg accgcggtag gaccggagca
     1261 ggtccactcc cgccacgtcc aggcccagca ggcgagtggc gcggatcgcg atggattctt
     1321 cctcaggggt cagcctgacg ggctgggctc tggcgccccg gtgcagattg gagcggaact
     1381 ctcctttttt cgcctgtctt ttcatagcgc ctgccacccg tccatcaatc acgatgatcc
     1441 ggatatcgct tccattcact tcctggataa attcctggat gatgacttgt tcttccagcc
     1501 gttgaaaggc ttccaggata ttcagggcct gggctttgtg attggctttc aataccccca
     1561 tgccctgggt gcttttgagc agtttgatga tcaggggata tttaaatttt tgcaggactt
     1621 tttcgttgta ttcagcattg aaatgcaggc aggcttccgg caccggcaat ccccgggcag
     1681 ccaggaactg actgcaactc catttgttcc tggccttcag caaggcatcc gaagtggtgg
     1741 tggaatatac ccccatggaa gtaaaatgat cgatgacgga agcgccgata tctgtagccg
     1801 tagctccgat ccgggggatg atggcatcga tgcgcatcag gggccgggag gcgtaataca
     1861 gcctgggttt tccgccaatg atatgctggc tgcaatgata agggtccagg acgagcatcc
     1921 ggtgattcct tttcctgcca gcaatggcca ggctctgggt tgaatagagt ccggggttgc
     1981 gggaaaggat ggcaaggacc atggagaagg agatttggct gattgcgatg gtaaatccga
     2041 agccaaaact atgactttcc cggtttattc atgattatct ttggccatgc ttccgttagt
     2101 tgaaaagaat aatcccaggg tggtccggag ttgggccatg tttgactggg ccaattcatc
     2161 ctacgccctg gtgatctctg ccgccatttt tccggcctat tttctgagtg tgacgaacgc
     2221 gtccatttcc gtgggaggca atgccgtgcc caacagctcc tattattcct tcatcatatc
     2281 cattgcttat atttttgtgg cggcgggatc gcccatcctt tccggtatgg ccgattacag
     2341 cggcaaaagg ctgtttttcc tgcgtttatt tacctggctg ggctcccttt cctgcatctc
     2401 cctgtttttt tttaaaggca tggagaccct ttggctcggt acggcgggct ttatgctggc
     2461 cagcatcggg ttcaccagca gcctggtgtt ttacaattcg tatttgccgg tgatcgctac
     2521 ggaagaacat tacgaccgga cttcggccaa aggctttgct tacggatata ccgggagcgt
     2581 gctcctgctg atcttcaacc tggtggtcat ccagaaaaaa gaatggtttg gaatcgagga
     2641 ttccggattt gcggtccgtc tcgcttttat cacggtggga tgctggtggc tgctctttgc
     2701 ccagatcccc ttcaaccggc tgccttccga ttcgaaagaa aaattcggaa aagggatatg
     2761 gcaaaaagga tattttgaat tgcttgcggt ttggcataaa ctgaaaaaac aggtgcacct
     2821 gcgccgattc ctgcttgcct ttttttttta cagcgcaggg gtgcagacgg tcttgtacct
     2881 ggcggccact ttcgccgata ccgtgctgaa tttcgggggc tctgaactga tcgtgatcat
     2941 cctgatcctt caattagtag gcctggtcgg ggcctatctg tttgccggag tatcccggat
     3001 gaaaggcaat aaatttgccc tgctcaccat gttgttcatg tggattgccg tatgcatcgc
     3061 cgcttactat acctatacga agttccagtt ttacctgatc gcttcaggcg ttggactggt
     3121 gatgggtggc atccaggccc tgtccaggtc cacctattcg aaactgctgg atgaaaatga
     3181 aacggacctc acctcctatt tcagttttta cgatgtcctg gaaaagctgg ccattgtgat
     3241 cggcaccttc agttttggat acatcgacct catcagcggg ggcatgcgca acagcgtgct
     3301 gatcctgatc gtctattttg ccatcgggat cgtgctcctc acccgggtca aaatgtcgga
     3361 agccaggagg gatgtcagga aacaatagtc aatagtcaat agtcagtagt cagtaatcaa
     3421 taatcagtag tcaagaatca gtagtcagta atcagtagtc agtattcagt attcatgaat
     3481 cagtggaaca ctgctggcaa ctcgtatctc ctttctcgct aatcagtgga catgttcgcg
     3541 agttagtgag atctttgctc gcatctcgta tcttgtttct cgtatctcgt ttctcgtatc
     3601 tcgattctcg tttctcgttt ctcgtttctc gtttctcggg tcagcttcca gctcaatcca
     3661 ccctcaatcc cccataactc agtgttgcct tgatcaggcc acccgtattt tccgccctgg
     3721 aatacccttt taccgatttg gaattggatt tttctacctg gtaggtgctg ttgaaatcgg
     3781 agggggtttt gatatccgca taggaaccgc tggcgtccag gcgatagggg gctcccccgg
     3841 tgctgatgcg cacatccgta taggaggttt tcagatcgag ggactgaaag tcggcatcga
     3901 ggtcccggat aatgatatct ccgtacgtcg aggaaaagct ggtccgtttg ccgactttgc
     3961 caaggtcaaa atccgtatag gcgctgctga cgtagatatc gccgaccgaa cgtacgcgga
     4021 tgtcgtcgta acggctttgc aggtccaggc tggcgatgta ttcggcattg agttcaccgt
     4081 aggcgctttc ggctttaatc gtgccggctt tattcaggtc gatctcggaa tatctgaaat
     4141 tgaggcttcc gtcttccatt tccgccaggg ttatgtcgga gtacctcagg ttgagatggc
     4201 cccgcttgat ggaacccagg ttgccgtcgc tgtaggccag ttcgaggtcg aggtcggcgt
     4261 tgaggccgtc catgcgcagg ttgccgtagg atatttttac ttccgcgttg cggtccagcc
     4321 ggtccacgaa ggcatcgccg tatttatgat tgagtttcag gtataggtcc gccgggaggt
     4381 atacgtcgta gttgatcttg taggaggaat ttttattcca gccccccacc cagttgcgga
     4441 tgtcccacca gtttttgttg gtggactgga tcacggtttc tgccttgata taattgctcg
     4501 tggtggtaaa ctggatctgg attttttcaa acaattcatc cgcttcggat ttggaattgg
     4561 tgttggcgat gatgttgaca tcgacggagg cgatgcgttt ttcccaggtg tgcactttga
     4621 cataaccgta tttattgatc agttcgaccg tggcgtcttt gggcacgtcg tattccttgt
     4681 ggtaattttt ggtaaattcc ccgtcgctgg cataagccgg caagaggaac agaaaaagca
     4741 ggagactata aatcgagcgt atcatgttct ggtttgttat tgggtaagga aggattttca
     4801 ttttcttttt gttgaagttt tgacaggatc ttgttcagga tatttaattt ttgattgtat
     4861 tgctgcatca tttgtttgac caggatgctt tggtcctggt cggggttttt caggagttcc
     4921 agttttagtt cagcggaaat ctgatcgatc tggttgaggt cgtccaggac ggaagcgctc
     4981 gatttatatt tcccgagttc cgtgagtttg tcgtcgatct ggctggtata ataggcttcg
     5041 gcttcccgga attcctggcg ggtgtccttg ttttgaatgg cataggtaaa atctttttgc
     5101 cgctggatct ggtacccgac cccgatgccc agggacagga ggataaggcc ggaggctgcg
     5161 attttgacga tggccgggat gatgccccag agggatttca ccttgccccc ctggtgcaat
     5221 tcggcttcga tccggtgcca gacctgccgg ggtggttttt cggtttcaaa ttccgcccgg
     5281 tgttgctcta tgaatcgttt cagtttatcc tcactcatgg ttttaattta ttttatcatt
     5341 tggtcattgt atcgacaata attctttcag tctcaccctg gcccggctgt actgggattt
     5401 cgaggtttcc tccgaaatcc ccaggatctc tccgatctcc tggtggtcgt acccctcaaa
     5461 caggtatagg ctcattaccg accggtatcc atcaggcaag gccttcatgg ccgatttgat
     5521 ccggttgatt tttaattcct gttcttcaaa atccgtttca tggccctggg ggaggacagg
     5581 ttcatgattg ctcagttcca ccagttgcaa cttccggctc tttagcttgt tgatgcaacg
     5641 gttgactacg atacgcttga tccatgcgcc gagcgtagac tcgaaccggt aggattgcaa
     5701 atgggagaaa acatccacaa aagcctcctg cagcaggtct tccgcttctt ccggtgcgtt
     5761 gagcatccgg aggcagatct ggtacatcgc ctgcgcgtac cgttcataaa gcgcagactg
     5821 agcggatctt ttgccggcta aacagtcttc gacgagagcc cgttcttccg gtctatcttc
     5881 cctgtcagga gtggacattg atatacatta aagagagcgg gccggctaaa aggttgcacg
     5941 gcccgtaatt tttttttatt cttcctcgtc ccgcgttccg ggcccttccg gtccgggcgc
     6001 ctggtccctg atctttttgt cagaaacatg acggtccaaa aaccggttga ggtcctcgat
     6061 attgagattg cttttcagct ccccaaactg gttgatctgg atgtcaaagc cacggatgtc
     6121 tttatggact ttggaacccc gtttcttttt catttttcaa atttaagaat aaagtccaag
     6181 tccggggatg gtcctgttaa tcccgggcca caaaagtccg gatcctgctc aggccctgtt
     6241 tcccaagaat ttggagcatc tgaaacaccc ccgggccctg caaaccgccg gccagggcca
     6301 aacgcaggat gggaaggatt tgacccggtt tgacgttcag ggattggaac ccggccttgg
     6361 cagcctgttc cagggcctgg gcttcaaagg gttcgaccgc ctcaaggatt ttcaccagtt
     6421 cggagatgac gggtccgagt tcgggcttcc agtgtttgcg gaccgcttct tcgtccatgg
     6481 tccgaagttc agaaaataaa taccctgcgg catccggtat ctcgctgacc aggttgaccc
     6541 tttctgtaaa cagcgggaca atttccaggc atttccgttc gtcccaaagc catccgcggg
     6601 cttcaaccag aggcttgagt ttggagacta tttcctggac aggcctttgt tttatgtatt
     6661 ggtgattgaa ccacagggct ttgtccatgt caaaacgggc tcctgagcgg tgtacccggc
     6721 ccaggtcaaa agcttcaacc aattcatcca gggaaaacag ttcctggtcg gtcccgggat
     6781 tccagcccag gaaggcgagg aagttcagga ccgcttccgg ctcaaatcca aattcacgga
     6841 atcctgtaaa actttcttcg gctgtgtccc ctttccagga caaggggaag accggtattc
     6901 caaaccgggc gccatccctt ttgctcagtt tgcctttgcc atcgggcctc ataatcaacg
     6961 gcaggtgggc ataacgcggc ggggaccatt caaaggcctc atacagaagg aggtgatggg
     7021 ccgtactgct cagccattcc tctccccgga tcacatggga tatgtccatg tgatgatcgt
     7081 cgacgacatt ggccaggtgg taggtgggca tgccgtccgc cttgatcagg accttatcgt
     7141 ccagttcggt ggtgtcaaac cggacctctt cgcggatgag gtcccggtac accacctgtt
     7201 tgccgggatc cacccttaaa cggacgacat ggggtgtgcc ggctctgagc aggtcggtca
     7261 cctccagcgg atccaggctc aggctgtttc tcatttccat ccggaccgag gcatcgtatt
     7321 tgggagcggc caccccggca tccgccagtc tggcacgcat gcggtccagt tcttcttccg
     7381 tatcgaaggc atagtaggct tttcccgatt ccaccagctg aagggccgcc tcccggtaga
     7441 cggctttgcg ctcggattgg cggtaggggc cgagtttgcc gccatacccc tggccctcgg
     7501 tgggctcaat gccgcaccag cgaagggcgt ccaggatata ttcttcagct ccgggaacgt
     7561 accgggcctg gtcggtgtcc tcgatgcgca aaatgaaaat gccatggttt tttttcgcaa
     7621 acaggtaatt atacagggcc gttcttacac cacctatatg caaggctccg gtgggagaag
     7681 gggcaaaacg gacacgaacg gacataggcg cgggtttgtt aaataattat attaaaaaaa
     7741 tatctaatat gaaagatcca ttctattttt gttcatctat taaaatgcgg tcagcaggca
     7801 acggttaatc acaacctgtc gttataacta atgctgatgc attttgacaa gatgcgcagt
     7861 aatccctttt ttctcatgaa cgtagcagac gattatgtat ttaattaaga ctccaaaagt
     7921 aattcaaaat ttcttcccca attttatctg gcaggtacct acccgggaga aagccctctt
     7981 cctgaccttt gatgacggcc ccattcctga agtgactccc tgggtcctgg acgaactgga
     8041 aaaataccag gccaaagcca cttttttcac ggtggggaac aatgtccggc agcaccccga
     8101 tatttttaac cggatcctcg accagggcca ttccgtaggc aatcatacct ataaccatat
     8161 caatggctgg acttcggaaa acatccctta tttccacgat gtcagacatt gcgccaaact
     8221 ggtacattcc tcccttttcc gtccgccctt tggccgcctg aaaccgggcc agatccagtt
     8281 tttgcagcgc cattaccgga tcatcatgtg ggatgtgctg agcggcgatt ttgaccagga
     8341 aatcacccgt gaacaatgcc tgagaaatgt attggacaac gccggggaag gcagcatcgt
     8401 ggtcttccac gacagcctta aagccaaacg caacctttgt tttgtccttc caaaagtcct
     8461 ggactatttt tccaggctgg gctacacgtt cgaatgccta agcccccatt ccatccacca
     8521 ggggattttt gaaaaaaaaa ggattacggt atagcattca gctttcagca ttcagaattc
     8581 agccgatggc tgacggctaa tagctgacgg ctcatagctg atgtctgata gctgaccgca
     8641 gaccaccctt cccatttgtg aatgttttat taattcatca gggccgcaca acccggcccg
     8701 attattttgg tattttagcc ttcgatgacg gacgggggat ccatacaggc ggtaaatcag
     8761 ttgagacagc tggaaaggga gttgcttctt aagcaaatgc agatccagcg gctgctgaac
     8821 attacccagg ccatcaacag caatatgtcg gcggatgacc ttttcaatat gtaccgggaa
     8881 ttcctccgtt ttgaaatggg tatgtcccgg atcctgctcc tgatccagga agtggatcac
     8941 tggaagctgg ccgtggaatt taaccttgaa aaaaaatata ccgcggaggc cttggttccc
     9001 attttacgaa acattaccaa tttatatacc gtaaaagcca ccgatgaaga aatcctccat
     9061 gaattcgaca tcgtcatccc cgtataccac aaggagcatc cgatttctta cgccctcgtg
     9121 accgtggcca ataaggagga cctgtatcat aaaatccagt ttatccagac catcaccaat
     9181 attgtcgcgg tagccattga aaacaagagg cttttcaaaa accagatgga acaggagcgg
     9241 atgaacaagg agatcgaact ggcccgggag gtgcaaagcc tgctgatccc gcaaaccttt
     9301 ccccggagca aaaactttga actttcaagc atttacaagc cccacttcaa tgtcggcggc
     9361 gattatttcg attatatccg catcggggaa gaccggtacc tcatttgcat ggccgatatt
     9421 gccgggaaag gagtgtcggc agccttactg atgtccaacc tgcagggaaa cctgaggcaa
     9481 ttggcccggc aatacacaga tcccgaacaa ctggtgatcc agctcaatga ggccgtcttt
     9541 gcattgaccg ggggagaccg tttcctgacc ctgttcattg ccgaagtgca gccggcccgg
     9601 ggcaggatcg attacatcaa ttgcggtcat atttccccca tcctatattc cgcggaccgg
     9661 gtcgaatacc tctcgaaagg gacgacggtc ctgggggcct ttccaaagct gcctttcatc
     9721 gagcatgggc tgatccgcgt ggaagaccct accattttac tggtattcac agatggcctg
     9781 actgacctgg tcaatgcctc ccatcagtat tttgaaatcg agaacctcga aaaatttgtc
     9841 cgtgattttc acggcgaaag tgcagaggca tttaatcaac gcctgatgat cgatctcgaa
     9901 aaattcaaag gcaatcaaac cttcccggac gacatcacgg tgctgacctg caaaattgtg
     9961 ccgaatgctg tatcttgaac cctggtttca aacatgaaaa acaggattgt tgccggtctg
    10021 ctcgcaatat tattgggttc agtcggagct cataagtttt atttgcgcaa gccgggtgcg
    10081 ggcatttttt atatcttttt atttatgatt ttcctcaatg cccggcttcc catcatggcc
    10141 atcttgggcc tcattgaggg attgggcctg ctgatgatgt cggaccagga attcaaccgg
    10201 cggtacaatg cgggccggat ggttggccag gaaggatacc cggaccaata tcccggtccc
    10261 agggaaatcc gctcccgcga aaggatgcgt gaccgttcgg aaagaagggg agggggctgg
    10321 aagaatgtgg aagtccccga atatccgccc aaccgaagaa atgtattcaa ggaaaccggg
    10381 atgcagaaat acaaggagtt tgacctggaa ggagccattg aagactttca gaaatccctt
    10441 cagatggaac cccgggacat cgccacccat tttaatatag cctgtgcttt ttccctgaca
    10501 gagcagccgg ccaaggccta tcatcatctg tccaaggcgg tggagcttgg attcaatgat
    10561 tatgaaaaaa taaataccca cgacgacctt gcttttgtac ggatacagaa acaatgggat
    10621 gggttcaaac agagcgggta ccgccaatac gccgcttttg agaaccagcc ctttgcagaa
    10681 gaaaaacccg aaccccaaaa cgacctttta ctggaccagt tgaacaaatt gaacgaactc
    10741 cgccagaagg gcctgatcac cgaagcggaa tatgtcatgg aaaaaagaag attgaacgaa
    10801 taataacatg agtttaatac ttccaagtcg ccggtttgcg tctggtgttc aaatgaaaag
    10861 gtgatgctag cacatgatct gattgccgag gggattcccg ccatagaccc gcaccgggtt
    10921 ggagaggagg ctttgcggcg gatggaggaa tatgaggtga tgcacctccc ggtggtgggc
    10981 aacgggcaat ttatgggcct gctcagtgaa gaggagatcc ttaagaacgg agtggacacc
    11041 cccgtggaga cctatctcga tgaaatgtcc aaagtctcgg tccacagaaa cgatcacctg
    11101 tttgacgtga tgaaaaaaat gacggattcg gacattactg ccgtccccgt caccgatgaa
    11161 gaagggcatt acctgggcct gatcacccgg aaggacctct ttgactttta tggtagaagt
    11221 tttgcctggt ccgaacccgg cagcatcctg gtccttgacc tggaagacgt tcattattcc
    11281 ctgtctgaaa tatccaggat catcgaagcc gaacagggga tgatcatcag cagcatcatc
    11341 agccggccct ccgaaacgag gatccttctt acgctcaaga tcaaccagct ggacattacc
    11401 cggatcattg ccgccctgag gcgttatgat tacgaagtca ccgcgagtta caccgaggcg
    11461 gaggtcgcag accatttcaa agaacgctat gacgccttca tgagatatct ggatgtctga
    11521 ttaccggacc atgttcagga tgacgatcgg tgtcttgttt ctggccgcct tctgtcaaag
    11581 ccaggcccag tacctttaca tcgattccat ccgtttcgag gggaataaaa aaacacaaaa
    11641 ctatatcctg aggcgggagc ttgatttcag ggttcatgat accatcgccc agcaatatct
    11701 ttctacacga cttcttgaaa accggagcag gttgctgaat accggccttt ttaaggaagt
    11761 ccatctcaac atcagccggt gggatacgga aaccaatcat gtgaccatgg aagtccgggt
    11821 ggaggaggcc tggtatatct atccggttcc tgtctttgaa ctggccgacc gcaattttaa
    11881 cgtctggtgg aatgaattca acggatccct caaacgggtc aattacggcc ttcgctttat
    11941 acacaataac cttaccggac agcaggacgg gctcaaggcc accttgcaac tgggatacag
    12001 tcccaagctg gaaatgatct attcctggcc tttttttggc agggaaaggg cctggaggtt
    12061 caggtcggaa ttattgtatt cccaaaacag ggaatcttat tacaagaccg aacaggaccg
    12121 gctggtcttt caccgttcgg agtcggattt tgccctgcaa cgcctgcggt tcttatccgc
    12181 cctggagcac cggaccaccc ttcagctgtt tcaaagcatt tcactcgaat accagttcaa
    12241 cagaaccaac gatctgatcc gaaatgtgct taaccctgat ttttttcttg ataacaaggc
    12301 acgccagaat tacctgaccc ttcgctaccg gtttatctac gacaaccggg acctccgctt
    12361 ttacccctcc cgtgggcttt cggccgaatt ccaggtggcc aaggaagggc ttgccggggc
    12421 ggaagatgta aacacgctga atgcaaccat ccagctcgac cagtactttc cctggaccac
    12481 ccgtcacaat gcggggatca tgcttaaagc caagggatcc atcatccggg ataagatccc
    12541 ttattacaac agcaaagccc tgggttacgg ggaaaattat ataaagggtt atgaatatta
    12601 tgtcatggat gggatggatt acctgttttt aaaattcagg gaacgctaca ttttttttga
    12661 ccgggccatg gccttcagga aaaacaaaaa aataggtgtt aaatccatgc cggtaaaact
    12721 cagtacggcc ttgcatgcga gtaccgctta tgtcaataat gtgcattatt ccttcaataa
    12781 tcccctggcc aaccgctggt tatacagtat gggagccagc ctggaatgcc tgctggccaa
    12841 cacttccctg atccagctcg acttcagcat aaaccacctg aaagacaccg gaatttattt
    12901 acatttcagg gatcaattct aagtatgcgc atattgttgt ttgcactcga gacccgggaa
    12961 gaaacccgga aaccattgat ggcccttacc gatgccttgt tggaccagcc cggtttcgaa
    13021 atctaccgct atggggaacc ggtggcggaa aacgaaaccc gttacaatga gctgcccctg
    13081 attcagagcc agcaggaatt gctggaacac cggatagata tcatcatcac cgccggcggg
    13141 gacggcacca tgctcaaggc ggctgcgttc tccggggcaa ccggcatacc cttgctgggc
    13201 tataacctcg ggcgcatggg tttcctggca gtggtcgaga aggattccat tcccatgatg
    13261 atttcctctt tgatggaaag gcaataccag atcgaagaaa gaattaccct ggaagtcacc
    13321 tcagatccgg tgatcttccc ggaaggaacc tttgccctga atgattttac catcctgaaa
    13381 agggacaatt cctccatgat taagatccat gctgccatca atggggaatt cctgaatact
    13441 tactgggcgg acggcatcat cctggctaca ccgaccggtt ccaccggcta ttcgctcagt
    13501 tgcggaggac cgatcatatt tccccaatcc ggcaattttg tgctcactcc gatcgctccc
    13561 cataatctca atgtcaggcc catcgtgatt tccgatgatt ccgtcctgga attcagggtg
    13621 gaaggccgta ccgatagttt tttatgcacc cttgattccc aatacgccct gatcaactcc
    13681 gaatataaaa tccggctcag aaaaaattcc ttcgccatac gcctggtccg gctcagcggg
    13741 cataccttcc ttaaaaccct gcgcgataaa ctgatgtggg gtctggatat gaggaattga
    13801 gtagcgagta gcgagtagcg agaagcgaga aacgagaaac gagaaacgag aaacgagaag
    13861 cgagaaacga gaagccagat acgagaaacg agaagcgaga tattcccttc ctcccgtctc
    13921 ctgtccccct ttctcctaaa atcactgccg agttaattcc cttccacttt gagttaatat
    13981 cgtattagag accattttct gcaccgattt ttttggattg atatttccag aggttggtcg
    14041 aggggatatt caccttagtc gcaaaaaggg ccataccggc cccggagttg caaccatttt
    14101 cgtttaaaat gggtctttaa taactgtatg aaatccttaa ctaccattgt ttttgctgca
    14161 gtgctctatt tgacctgcac ttcttctgcc cttgcccagg atatgatcac cggcaaggtt
    14221 gcagattcca agtctgtggc ttatgaattt gccaccgtct tattgctaaa tcacacggac
    14281 agcagcctgg ctaaaggcgc cattaccgat cccgaaggaa gtttcgaatt tgagaatgcc
    14341 aaacccggca aatacctgtt caaaatttcc tatgttggta aaccggatat ccttacccag
    14401 gcctttgatt ataccggagg tatgctccaa accggtgttc atgcttttcc ggaatccgcc
    14461 accgaactca agaccgtgac cgtaagcgcc gtaaaaccgg tgttggaaat caaagctgaa
    14521 aaaatgattc tcaatgtcga gaacaacgtc agctccgccg gaagcgacgg cctcgaaatc
    14581 ctccgtaaag cgcccggggt tgtgatcgac aataacgaaa acatcacctt gaaagggaaa
    14641 aacggggtcc aggtttatat cgatggcaaa ccggccaggc tcgggggccg agaccttgcc
    14701 gccctgctca aaagctacaa tgccaataat attgaagcga ttgaagtcat catgaaccct
    14761 tcggccaagt atgaagccga aggcaatgcc gggatcatca acatccggct gaaaagaaac
    14821 cagagcctgg gcaccaacgg taccgcggcc ctgaatttca ggatgggttt tacccctaaa
    14881 gggaatgctt ccctgaacct gaatcaccgc aaggaaaaat tcaattttta taccaacctt
    14941 actaccgcca tgggaatcaa ccatgaaaac gtggatttct accgggaaca gggcgggctt
    15001 ttcttcgacc aggtcaaccg gatcaaggac gattcggtac gttttccgcg cttcaattac
    15061 cgtttcggcg tggattacct gatcaatgca aagcataccc tcggagccca gatcaacggg
    15121 aatgtatacg atggggtcat gaacagccat ggccgaaccc ggatcggcac cctcaacaat
    15181 ttcggcatca tcgattccat cctcaatgcc aatacccgga ccctggaaac ccgcaataat
    15241 ttccagacca acctcaacta tgcttataag gatacgatgg ggcggaactt taacctcgat
    15301 gccgactacg gcaggatcaa ttccaaaaac gacagttaca accccaacat gtacatgtct
    15361 ccggccgaag acgccacctt gtcctcttcg atcttcggat cttacaccaa taccgatatc
    15421 accatcaaaa ccctgaaggg ggactacgaa cagaacctgg cgaagggccg gttgggcatt
    15481 ggctttaaac tatcccaggt cacgaccgga aacgtattcg acttctttga ttacatccag
    15541 ggcgtaaaaa cccaggacct ggagaaaagc agcgacttta attacgacga gatggtgtac
    15601 gccgcgtatg tcaattacaa caagcagatc aaaaaatggg cggtccaggc gggattgcgg
    15661 gtcgaaaaca ccaaatcgca cggccagttg ataagcctca atcccaagga cgaagacgac
    15721 gtaaaacgtt cctatgtcga tgcctttccg tcctttgcca ttacgtacaa tgccagcaaa
    15781 aaacatactt ttggcctgac ttacagccgc aggatcgacc ggcccaggta tcataacctg
    15841 aacccttttg aacaaaggct ggacgaactg actttccgca agggcaatcc cttcctgcaa
    15901 ccccagtaca cgcagaacct gaacttcaat catacattaa tgggtatgtt gaccacgacc
    15961 ctgacctatt cgaggaccaa cgactttttc ggccaaatca tcgactccac ccagcgccgg
    16021 aggacattta tcaccgagaa aaacattgcc cagatggaca attacagcat cggcatcgcc
    16081 actccgattc cactggcaaa atggtacaac gggtatatca gcgtgaacgc tttccggcag
    16141 atttacgaag cggattttgg aacaggtaag aaactgaata tcgccaccaa tggatttaac
    16201 ggttattccg aacatacctt taccctgaac agtggcttga aaatgcagtt gtcgggctgg
    16261 tacaaccacg gcggcgtatg gggaggcacc tttgtcggca aaagcatggg aagccttgat
    16321 ctgggtctgc agaaaacctt cctgaaaaac aaggtggatg taaaattgtc cttcaccgac
    16381 gtcctgaaaa ccgcagggtg ggatgccatc agtgatttcg gcggtattta ttttgttggc
    16441 aagggttaca atgaatccca gaccttccgg gtcaacgcca cctaccgttt tggaaatatg
    16501 aaagtgaaag gcgcacgcca gagaaaaacc ggcctcgaag acgaacagcg cagggccgga
    16561 aactaaggac cgcggacgag ggcttccacg ccaagcccta ctcatcccat aaataccaaa
    16621 aaaccggtta agccggaaag gggttccctt tccggctttt ttattccgga ggttgggcct
    16681 tacccgaatt cttacggatc attgggcatt cgcccttact tttgccctgt gttatccagc
    16741 agcaacttat cgttccaata ccccggggga agggtcatgc agtttcccga catccatgtc
    16801 caggacaaag gccattgcct gatcctggga aagtccggca cgggtaaaac caccctgctt
    16861 catttgttgg ccggcttgct gcccttgcag accggagaga taaagatcga cgatcaatgg
    16921 gtgaaatccc tgggagccag ccagcttgac cggttccggg gtaaaaagat cggaatcgta
    16981 tttcaaaaat ccatttttat cgaggccatt tcggtcatgg aaaacctggc ctgggcacaa
    17041 cacctggcgg gcaatccggt cgatcacggg cgcatcctcc tgctcctgaa aagactgggg
    17101 gtcgaacacc aggcgcaccg ccttccttac cgcctgagca tcggggagca gcaaagggtt
    17161 tccattgcgc gggccctggt caataaacct tccgtgattc tggctgatga acctacttcc
    17221 gccctggacg atcaaaacgc cgatgccgtg atccgcctgc tggaagaaca ggccgacctt
    17281 gaaagcgctg cgctcatcct ggtcacccac gatgcccgca tcaaatccca ttttgaacaa
    17341 caaatcatcc tggcggcatg aaactactga aactgagttt tcaaaacctg ctgcataaac
    17401 ccttgcacct gaccctcagt ctggtccttt ttgcgctggg agcaggactg attgccctta
    17461 tggtgacgct gaacaaacag attacccaga aatttgaaag caaccttgcc ggcatagacc
    17521 tgatcatcgg cgccaaaggc agcccgcttc aactggttct caacgggcta taccacctgg
    17581 atgccccgac cggcaatatt tctttgcagg atgcgaagcc ttttttaaat cccaatcatc
    17641 ccctgatcaa aaaagcggtg cccttgtccc tgggtgacag ttatcggggg ttccggattg
    17701 tcggcaccaa tcgggatttc cctgaattgt ataaagccgg cctgaaggag ggtgtttggt
    17761 gggacaaacc tatggaagtg gttttgggga gcatcgtggc ctccacggcc aaacttaaat
    17821 taggggatac atttatcagt tcgcatgggc tgaccaccga cgattttaac cagcatgaac
    17881 acgcgccgtt caaagtagca gggatattga atgaaaccgg gacggtttgc gatcagctcg
    17941 tccttacttc agcggaaagc atctgggaag tgcatgaaca ccaggccaaa cccgccctgg
    18001 tagaagaggg ggaagataaa caggatcccg ttgcaacaga agaaacacac gatcatgagg
    18061 aaggggaggc acacgatcac gagcacgaaa accaagccga ttccgccacc ttggcctcac
    18121 gggagatcac caccctgctc atccagttca aggccagaaa cgtacaaacc ctcaacctgc
    18181 agcgtaacat caaccagaat acccaactcc aggccgccac cccggctatt gaaatcaatc
    18241 gcctgttttc ccttatgggc attggtttcg atgccatccg tgcactggcc tggatgatcg
    18301 ccctcgtatc cggcctgagc atcttcattt cgctttacaa cagcctgaaa gaaagaaggc
    18361 aggaacttgc actgcttcgc gtcctgggcg cgaccccggc gaaactgttc gccctgatta
    18421 ttttcgaagg cctgatcatc tccattctgg gttatctggc cggaatattg attgcccacc
    18481 tggtcatgca tttcagttcg gactacctgt tccaactgta caagtaccgt ttcgatgcct
    18541 ggcagtttat ccgggaggaa gccgtcttac tcctgggttc gctggccatt ggcgcggcgg
    18601 ctgccgtgat cccggccata caggcatacc ggaccgatat tgccaaaacc ttaagcgatt
    18661 gaccggaaac cgccggtcct ttacaagcct tccggatttt tacttttgat cattcggaac
    18721 caaagttcat ttctcaacgg aggctgaccg acttaccctc tttcgccgcc agttttatgg
    18781 cttccacgat ccggatgtct ttcaagcctt cttccccggg caccaacatc ggttttttat
    18841 tgatgatcga gtgcgcgtca tcgtccattt gtttggcctg ctggttgttg atcgttttat
    18901 tcagggtttt gccgtcgctg gtcattccct tgaccccatt gtaagcctgg aagggctcga
    18961 gcttgtacca gcctttttca ccattgacct gtaaagaatt catgcctttc ccaaagctgg
    19021 caaggccttt cccggtgacc ccgccaggga attcgagggt aaactccatg gtttcctcca
    19081 cctcgtgata aatttccggc cgggtggtgg aggctttggc ggaaagcacg gcgagcggtt
    19141 ccttgcccgt agaataacgc aaggcgttca acggatacac acccatatcc agcatgcagc
    19201 ccccgcccat tttttttatt tgtttccagt gggtggtcct gccatcccgg tatcccgctt
    19261 cggcataggc gtctttgatt gggccatagg gcagggtccg cgtgtattgc atgatctccc
    19321 gggtattggg ttcgtggtgc atgcgatagc cgatgctgag gctcaccttg ttttttttgc
    19381 aggcatcgat catggcctgg cattccgcgg ccgtgacggc catgggtttt tcacaccaaa
    19441 cctgtttgcc tgccttcgcg gcccggatca catattcttc gtgcatggaa ggggggagta
    19501 ctatataaat gacatcgatg tccggattgt tggcaatttc gtcaaagcgg gtatagtcgt
    19561 aaatgttttt atccgccagt ttatacttgg ccttccaggt ctcggctttg gagggggtgc
    19621 cggtgacaat gccgttcagc atacaatgct gggtcaattg cagcgcaggc gcaaggatgt
    19681 ctgtggaata atagcccaat ccgaccaggg ccacccccag tttttctttg ttccggggga
    19741 tgaggatatt gggcatgagg ccaggggcca tggtcatgct gccggccgcc atgccggcgg
    19801 ttttaataaa gtttcttctt ttcattggat cggttttaaa cagtatactt gcattcttcc
    19861 gaatgacttt ttcaaaaata acggaaaata gggcaaatcg attccgaaga gctttgtatt
    19921 cctggaccgg cgaacatccc agggagatgc cctggaaagg ggagagggat ccctataagg
    19981 tctggttgtc ggaaatcatg cttcagcaga cccgggtgga gcagggaatg ccctatttta
    20041 accggtttat cagacggttt ccggatgtgc aggcactggc aaaagcttcc ataaagcagg
    20101 tcttgaaaca atgggaaggg cttggatatt attccagggc aagaaatctt catgagaccg
    20161 cccggtggat cgtacgctcg caggccggcc gttttccgga ggaatattcc tcgatcctcc
    20221 ggatgaaagg ggtggggccc tataccgcgg ccgcgatcgc ctcttttgct tttggccttc
    20281 ctcatgccgt gctggatgga aatgtacacc gtgtcctttc ccggtatttc ggcatcgatc
    20341 aaaccatgga tcaccgggcc ggaaaaaaat tattcgaaga tttggcggac cggttgctgg
    20401 acaaggagca gcccgcgcgc ttcaaccagg ccatcatgga tttcggagcg ctctgctgca
    20461 cgcccaaaaa cccggattgc acttcctgtt atttgagaaa ccagtgtttc gcatttaagc
    20521 tggaccgcat cggacagttg cccccggcaa aaaaaagcaa gcccaggcgg atcaggcatt
    20581 ttcattattt tattgttgcg gaccccggtg gccaggtggt gctttcccgc agacccgccg
    20641 gggatgtttg gccgggctta tatgaatttc caaaggtgga gaccgatggc ccccaggccc
    20701 caaaagtcag gccggcgggc gtggatttgc caccccgg
//
LOCUS       DAOQXT010000085        20665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2945_length_20665_cov_6.889859, whole genome shotgun sequence.
ACCESSION   DAOQXT010000085 DAOQXT010000000
VERSION     DAOQXT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20665
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2945_length_20665_cov_6.889859"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1265)
                     /locus_tag="PKM27_14000"
     CDS             complement(81..1265)
                     /locus_tag="PKM27_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08427.1"
                     /translation="MFKYKISLSLFILSLTFFYCKQRKWSNELKYKFNSSISNNKLKF
                     TDLLDSINFIQLDNSELKLGYPLERFHVRNGKYLIAWNESGGYGKCGLFDPKGNLIAH
                     LTSNSDPKLAIGSISDLDLLKDTLFVLSGTKLYKINVNNNSVLEQLNFGEMFSTFRST
                     EYGLAMFSQMNSEYAVNMFDSRGRFMKGLINLKELPTFISDDFRHFSVFDHKSDLFYC
                     NLSRDIYKIDYQLMNSEILISMDFGVNQLNNKTLYEYLKSAGADHEMQDKLFNKEGKF
                     NFYSKVFNLKSLYFINFKLNKKTYYSLLNKKSQNLVSYSTIDNDTGLNFLTNKFGAIQ
                     FMEGINEALYSIAYPESIHQAILNNNNQSSIKIKSNWNAFKEILNSNGNPVVVIYYLK
                     KF"
     gene            complement(2536..3627)
                     /locus_tag="PKM27_14005"
     CDS             complement(2536..3627)
                     /locus_tag="PKM27_14005"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNR08428.1"
                     /translation="MNKHSGPAGPGPHGIPDYIVTPKKLGYYFPAEFHPHESTWLSWP
                     HKEASWPGKLNTVYPPYVQFILELTRGEKVNINVNDQAMKDFALVELTKAGVDLEKVR
                     FYFHPTNDAWCRDHGPAFLIHPDKEKGKIIVDWGYNAWGNKYPPYDLDDEIPTWVGLE
                     LKLPVYYPNIVMEGGSVDFNGKGTLVTSTACLLNKNRNPQLNQGQIEEYLYAYYGVDQ
                     ILWVDEGIVGDDTDGHIDDTVRFVNEEAVLVVRENNKEDENHALLEHNFRQLKSMRLL
                     NGKPLEIIELPMPDPLYYEGQRLPASYANFYIANRSVIVPTYRCAKDDLALSIIQSCF
                     PDREVIGIDSTDLIWGLGSFHCLSQQEPR"
     gene            complement(3794..4669)
                     /locus_tag="PKM27_14010"
     CDS             complement(3794..4669)
                     /locus_tag="PKM27_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HNR08429.1"
                     /translation="MTKIVNVGLVQMSCTRDKETNLAKAIGLVREAASRGAQIICLQE
                     LFTSLYFCDTEDYEHFKLAEPVPGPSTERLQEVARETGAVIIASLFEKRAQGIYHNTT
                     AVIDAGGAYLGKYRKMHIPDDPAFYEKFYFTPGDLGYRVFETRFARIGVLICWDQWYP
                     EAARLTALRGAEILFYPTAIGWATTQDEATNAEQFNGWQTIQRSHAVANGIHVVSVNR
                     VGFEQEGAMKFWGGSFIANPFGRILYQASHDQEEIHVEALDLDKTDFYRTHWPFMRDR
                     RIDSYGDLVKRYLDE"
     gene            complement(4678..6162)
                     /gene="guaB"
                     /locus_tag="PKM27_14015"
     CDS             complement(4678..6162)
                     /gene="guaB"
                     /locus_tag="PKM27_14015"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNR08430.1"
                     /translation="MEKTTTSSSPVIEEMYTFDDVLLVPAYSQVLPREVNTESQLTTG
                     IKLNVPIVSAAMDTVTEKKLAIAIAREGGIGIIHKNMGIEAQADQVKSVKRSESGMII
                     DPITLKAGARVRDARLIMDKYGIGGIPIIDEGHRLIGILTNRDLRFETNFNRPITEIM
                     TKERLITAPKGTTLDLAKGILQQYKVEKLPVVDDTGKLIGLITYKDIMRLEDYPHSCK
                     DTFGRLVVGAAVGVNKELQDRVDALVQVQVDVICIDTAHGHSRGVLEAIKTIRQRHPN
                     LEIIGGNVATADGARALVEAGVNAVKVGVGPGSICTTRVVAGVGVPQLSAIMNASRGI
                     AGSGIPIIGDGGIRFTGDIVKAIAAGASTIMAGGLFAGVEEAPGETVLYEGRKFKTYR
                     GMGSLDAMQSGSKDRYFQDAEDDIKKLVPEGIEGRVPYKGLLSEVMIQNIGGLRAGMG
                     YCGAANIKALQQAKMVKISSAGIHESHPHHITITKESPNYSSKA"
     gene            complement(6234..7079)
                     /locus_tag="PKM27_14020"
     CDS             complement(6234..7079)
                     /locus_tag="PKM27_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNR08431.1"
                     /translation="MMFRLYKTPGRFIIEENERYFLSTAVDWDQWVNRKNLFEELKIE
                     IAGLQAMDKDAASAFLDKVLAPIGSQEVWASGVTYLRSRDARMEESEHAKSAYDLVYD
                     ADRPELFFKSLPHRVAGPGQAVNIRRDSTWNVPEPELTLFINSQGTIAGYTIGNDMSS
                     RSIEGENTLYLPQAKTYERSAALGPCLAIPGEPLGPETEIDLKIFRDNKCVFHGQTRL
                     GAMKRRPEELRDYLLRGLEFNEGCFLMTGTGIVPPTTFTLEEDDLVDIRIGTIGTLSN
                     PVKWY"
     gene            complement(7084..7674)
                     /locus_tag="PKM27_14025"
     CDS             complement(7084..7674)
                     /locus_tag="PKM27_14025"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNR08432.1"
                     /translation="MLEIQGKLVSTDLLEKNFVCNLQACKGACCIEGDEGAPLAEEEK
                     AILDRVSSQVAGFLDEESRTLLEKHRYVKLERGRYATPLRADGACIYALRDGAGILSC
                     ALETAYRFGKTDWIKPLSCHLYPVRIREDKVLGWEALNYDQWSICAPACTNGDQLGVP
                     VYRFVRNALIRKYGEAFYEELDEIAQNWKPETNSKK"
     gene            7846..9177
                     /locus_tag="PKM27_14030"
     CDS             7846..9177
                     /locus_tag="PKM27_14030"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HNR08433.1"
                     /translation="MDELKSIFRDKSQQLKEKIKAMLKEHGNMKIDEVRLEQAFGGMR
                     GIQSMIWETSLLDAQEGIKFRGFSIPQIQELLPKAPGGHEPLPEGIFWLMLTGEIPTD
                     AQVQWLSDEWERRAVIPRAMTLVLDALPEEVHPMTQFSIAINALQADSIFARRYEEGM
                     PKTEYWDATYEDAMNLIARIPFAAAHIYRRIFKGNKHIAADRSLDWAANYSHMLGFEG
                     QECHEMMRLYQTIHADHEGGNASAHTAHLVGSTLSDVYLSYAAAMNALAGPLHGLANQ
                     EVIKWIFDMVAALGTRKPTSEQIAAYVKETLNKGQVIPGYGHAVLRQPDPRFYAQKRF
                     SERYLAEDDIIQIVWRLFEIVPPILQSLGKVSNPWPNVDAHSGAMLVHYGLTEYNYYT
                     VLFGVSRSLGVMAAQCWARALGFPLERPKSLTSDWVFDFVQHNNDLNNVNS"
     gene            9188..9583
                     /gene="gcvH"
                     /locus_tag="PKM27_14035"
     CDS             9188..9583
                     /gene="gcvH"
                     /locus_tag="PKM27_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNR08434.1"
                     /translation="MSKIESGLKYTPEHEWFRLESGNRAVVGITDFAQGELGELVFVE
                     VPTVGKSLKKGDVFGTVEAVKTTSDLFMPIGGKVLEFNEAISESGGDNPGLINEDPYG
                     SGWIIKMEATDLSEVEGLLDGEAYQQLIH"
     gene            9623..9985
                     /locus_tag="PKM27_14040"
     CDS             9623..9985
                     /locus_tag="PKM27_14040"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNR08435.1"
                     /translation="MFFRKYLYSGIWILMILVLSLLPKSSLPEMEFDIPYLDKVVHFA
                     MYFILLVLVSWERQKLESKPLFFNGLMLALLLSICTEAGQRFFFAGRSFEILDIVANI
                     IGSISGIYFFRIILKPYK"
     gene            9982..10902
                     /locus_tag="PKM27_14045"
     CDS             9982..10902
                     /locus_tag="PKM27_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNR08436.1"
                     /translation="MNVTEIDGRVALIFVFAIFVAVVAIIFILKSVFRKKATEDLSSK
                     YKDKKWASPIEGMYKYPDVDVFGMSGPIWRFGLTAALGLAVLGLNWTTYDRKIDIPTD
                     ALAFDDEIQVEIPRTAEPPPPPPPPPPPVVQEIKVTEEKIEEKVEFVDQSIQEETVVE
                     APPPPEPKKAPPPPPPPPPPKEEPDEIFKVVEEAPRFPGCEDKGSTDEKKKCADEKML
                     QYLYKNIKYPNIAKENGVQGRVVVTFVVEKDGRITDARVVRDIGGGCGEEALRVVNSM
                     NEMGERWKPGKQRGNPVRVQFNLPVQFKLN"
     gene            11120..11521
                     /locus_tag="PKM27_14050"
     CDS             11120..11521
                     /locus_tag="PKM27_14050"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNR08437.1"
                     /translation="MDSIYQLAQEKPRFPGCEDLPGEIAAKQKCSEEKLLQYLYSKVK
                     YPQYALENNIEGRCVISFVVEKDGSISQPRIVKDIGGGCGEEAIRVIQSMNEMPERWI
                     PGRQDGLPVRVKFNLPVKFQLKESDEQEDAQ"
     gene            complement(11682..14660)
                     /locus_tag="PKM27_14055"
     CDS             complement(11682..14660)
                     /locus_tag="PKM27_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNR08438.1"
                     /translation="MIKALLLFFLLIVQVNSLPYSSPDTARQQAAFQEKKWVDSIFNS
                     LTTDQKIGQLFMIRAHSNLGPEHIAEVERQIRKYQVGGLCFFQGTPLKQAELTNKYQT
                     LSKIPLMVAIDAEWGLGMRFKESAISFPRQLTLGALQEDKLIYDMGVEIARQLKRIGT
                     HVNFAPVADINVNPANPVINDRSFGENRVAVTSKSFQYMMGMQDMGVMACAKHFPGHG
                     DTDVDSHLDLPVINHNRARLDSIELFPFRLLFEQGMQSVMVAHLQVPSLEPDPRTPTS
                     LSSSTMTDLIKNEYGYQGLIFSDALEMKGVTKNFTPGALEVVAFKAGCDILCLPDSID
                     LSFEELKKAVAAEAPLKLQLDEAVRKILHAKYKMGLNAYSPVSLEKLTDEVNSPKAIA
                     LKSKLYEQALTLVANKNKLIPLTELDQHKLASVSIGATEKSPFQWRMDSYAKVSHQQT
                     ELNIPEAKAAALLNSLKGKDIVIVSLHGLSKQLKQNFSLSPGTLNFIKMLEQQSRVIL
                     VSFGSPYALRNFETSSWVLQAYEEDPQMQDLAAQAIFGAIGCKGRLPVNSGPAFPAGS
                     GLDSESLMRFGFTIPERVGLNHKLLGSLDSLCHEIVNVHAAPGGQLLVARKGKVVYHK
                     AFGYHDYEKKTSVRTDDLYDLASITKVAASTLAVMRLYDEGKIDLDQPMSEYLPDLKG
                     TNKSRATLTEIMSHQAGFKAWIPFYKETLLRVKRHVYKPDPALYASANSSKYSLPVAQ
                     GMFLKTGYDHLIWKSILESSLRENNNYVYSDLGFYMIAQMIRYQTGMRIDQFVNKYFY
                     KPLGLTHTLYNPYQSFPLQKIAPTEKDNYWRNRVIRGYVHDMGAAMLDGVSGHAGLFS
                     NAYELGTLFQMLLNGGRYGGDHLIRSATISQFASRQLGSTRRGIGFDMKELNTEKTQP
                     AGQFCSPNTFGHTGFTGTSAWVDPDQELVFVFLCNRTYPSMENNKLHKMEYRTRLLDA
                     VYLAMEEERIPLDNTMQLGQ"
     gene            complement(14669..16618)
                     /locus_tag="PKM27_14060"
     CDS             complement(14669..16618)
                     /locus_tag="PKM27_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR08439.1"
                     /translation="MAKPGKNKTENHPIRALAPAGDPRFPWSSSTVYTVCFFLSLACY
                     GLTLGHDFVLDDALVITQNQFTQKGIRGIPEILSHDSFTGFFGEDKNLVAGGRYRPLS
                     LVFFAIEHAVSGAPWLHHLINVLLFGLTVMIIFGFLRTALARIYSPDRANWIAGLAAL
                     LYAVHPIHTEVVANIKGRDEIQAFLFGLATLWISWKPRPGLLRGSASLVCFTLALFSK
                     ENAILWLPAGWLAYIFLDRQDWVTATRKISLFIVPAFLFLSVRALVFRQILDSPLPME
                     WLNNPFLKITDGQYILCSASEKWGSILYALAYDLKMLVLPYPLTHDYFPNHFPLKKLS
                     DPSVLLSLLIHLGAFVVAWRNRKSMPVLSFSILFIYTSLLLTSNVFFPVGILFSERFL
                     YMASFGFCLGVAGWSYQFIQTVNKPALAIILLLIFVAAALTLSRGRAWKNNLTLFQTD
                     IRVSANSAKINNALAGELLALAASQKDSLGKIKLAAEAEGRLTKALQIHPLYSDAWLQ
                     KGNALFYQGRFDESILAYRRTLALYPEHTHAKANLHLALREEGKRQGEKLGRTDLALQ
                     YLSQAYSLKPDDFETLRLLGVAHGVNRDNQRAVEFLEKALALQPGNAAVLFNLGTAYF
                     NSGNAPKGREFYEKARLLDSTLFIQ"
     gene            16943..17152
                     /locus_tag="PKM27_14065"
     CDS             16943..17152
                     /locus_tag="PKM27_14065"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNR08440.1"
                     /translation="MDPGSLESNRNEELVADLFAQEGEFMGIPPTHKQVQFSGIVIHR
                     IEYGKFAESRNEIDLLGIRQQLTYE"
     gene            17210..17674
                     /locus_tag="PKM27_14070"
     CDS             17210..17674
                     /locus_tag="PKM27_14070"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNR08441.1"
                     /translation="MQPQNQKPLETQVSNIRSDSIKQWITRYLEEVWNKRDFTNADKY
                     WGPEFVNVFSPESGKGPEAMRQQVDYFLKAFENSHFELKNILVQGDQVALWIEISGKH
                     TGELFGIPATHKEVRFREAVWYTLKNGKLHEVYPFVDWNDLFRQLGKYPTLD"
     gene            17703..18680
                     /locus_tag="PKM27_14075"
     CDS             17703..18680
                     /locus_tag="PKM27_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR08442.1"
                     /translation="MKTIIRLSVLLLWFFNASGQAPLSLGSITNLYDAFGKERPGVAF
                     DFGFSCIINYNGTIILFDAGSNADTFARNVKALGIDLARVDLAVVSHSHFDHLNGMDY
                     LLQVNPGVKIYYPNDIFWGADVPFDATGQEPSIGDSLPKEMRYFGGGATKFSIKQSGR
                     FWEANVEYVKTSKEIAPGIKLIATTSPFLGYFNRYPNLSFVPGVFNDNNNNQSKANEA
                     GLPELSLSLATPSGEILIVGCSHSSVEKITAATREFTKQSIQLLYGGFHLLPYKREEM
                     SGMIHYLKDELGVREVAPAHCSGHLAFKMFGDAYGSGFHFAGLGETVRF"
     gene            19302..20531
                     /locus_tag="PKM27_14080"
     CDS             19302..20531
                     /locus_tag="PKM27_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNR08443.1"
                     /translation="MINELSKQLAREAMKQEILSPQEKLMEYESQEDKLFIGITREEQ
                     LLENRVALTPNSVMMLVSNGHRVIIERGAGKRSFYTDEEYAEAGAQVSEDKKEILKAS
                     IILKVAPPGIEEINMMPGDQMLISPLHMPLLTEEYIQALRTRRITAVAMEYLKDKHDS
                     FPIVRILSEIAGLSAILTAAELLSVTSHGRGVLLGGIAGIPPAKVLILGAGVVGEFAT
                     KAALGLGASVRVFDNNIYKLMRLQNSVGVPLHTSVLNPKYLEYQLRSTDVVIGAIHSK
                     TGRAPMVVSEDMVMRMKQGSVIVDVSIDQGGCFETSEITTHEHPTFIKHGVIHYCVPN
                     IASKVPRTASSAISNIIAPLLLSTSEVRNIDEMLFANPGLRHGVYTYKGHLTNEYLSR
                     RFNMKFTDINLLLTSSF"
BASE COUNT         5233 a         4984 c         5096 g         5352 t
ORIGIN      
        1 tcaattgatt tcttaactta gataattatt gtctgaataa tagggggctg aaccatctat
       61 tactaccgct tgcctttatc ctaaaatttc ttaagataat agattacaac tacggggtta
      121 ccatttgaat ttaaaatttc cttaaaagca ttccagtttg atttgatttt tattgaactt
      181 tgattattat tattaaggat ggcttggtga attgattcag ggtatgctat tgaatataaa
      241 gcttcattaa ttccttccat aaattgaata gctccaaatt tattagttaa aaaatttaaa
      301 cccgtatcgt tatctatagt gctgtatgaa accaagtttt gagatttctt attcagcaag
      361 gaataataag tcttcttatt aagtttaaaa ttaataaaat ataaagattt aagattaaat
      421 actttggaat aaaaattgaa tttgccttcc ttattaaaaa gcttatcttg catttcatgg
      481 tctgccccgg cacttttaag atattcgtac aatgttttat tattcaactg attaacccca
      541 aaatccatgg aaataagtat ttcagaattc atgagctggt aatctatctt gtaaatatcc
      601 cgactcaaat tgcaataaaa taaatcactt ttatggtcga ataccgaaaa atgcctgaaa
      661 tcatctgaaa taaatgttgg aagttcttta agattaatta aacccttcat aaatcttcct
      721 ctcgaatcga acatattaac ggcatattct gaattcattt gactaaacat agctaaacca
      781 tattcagtac ttctaaaagt actaaacatt tctccaaaat taagttgttc taaaactgaa
      841 ttattattta catttatttt ataaagtttt gtgcctgata acacaaataa agtgtcttta
      901 agcaaatcta aatccgaaat acttcctatt gctaattttg gatctgaatt acttgtcaga
      961 tgtgcgatta aattcccctt ggggtcaaac aatccacatt taccatatcc tcctgattca
     1021 ttccacgcga ttaaatattt tccattgcgg acatgaaatc gttctaaagg atagcccagt
     1081 tttaactcag aattatctag ttgtataaaa tttattgaat caagtaaatc agtaaatttt
     1141 agtttattat ttgatattga ggaattaaat ttgtatttta attcatttga ccatttcctt
     1201 tgtttacaat aaaaaaaagt cagacttaat ataaataggg ataagcttat tttatacttg
     1261 aacatattat aatgataatt ctatattaaa gtctgacttc atatttatta tagtaattta
     1321 cacgccacac ccacaggttg tttgtgctcc tacattacaa ttacagcttt cgcaagactc
     1381 ccaacaaact aaataataat tagtcgggca gccaggtgga cattctttcc atttaagtac
     1441 gtatggattt gattcacttg catgtgctat tgacaaagta tctgtaaaca tattcagtcc
     1501 aatagtaaaa attaacccca agagcaataa aaaaattttc tttttcataa ttataatatt
     1561 gaattgtgaa ataatggtgt caaagttcca acacatttaa aattggcctg aaaaatgtag
     1621 cccctttatc ttaaaatatc ctactttaaa acgatataaa attgctctag gaattcaagt
     1681 tgtaaatcaa tcacttagat attagtgatc aaccaccgta ttactatctg attctgaaat
     1741 atttagaccc aaatgtccat gtaacctcct tataatatac tcatattctg ctttactgaa
     1801 gtacttctga gaatggtaga gatgtaaaag ttctgggtca ttaactttat gtattctgtg
     1861 atttacaagg gacatactga tgcccagtgc ctctgtaatc tcatctttgg tgtagaattt
     1921 tgtcctttcc ataatatatt aagtgttaag tttattctca aggttatatt cattggggta
     1981 agaagtagta gaaattaata agtgcggtcg acgtgcggcc ctgtaacgga tacgttctca
     2041 tcagaatcca agtaccagac ccaaaaattt cacagcaagg gtgtattttg tcctaaatat
     2101 tagggagttg tgaatccatt gcttaacgag gtattgaacg gtattgattg gaaaaaaaat
     2161 tgaattgaat tgaattgaat taaatcgaat taacctgtac tcataccttt tactctcata
     2221 attattagta tgcattttag tgtgttgaat gttatttcag ttttgatcgg gtatctgcat
     2281 catgcccttt gagacatgaa ggttaattct caggattaaa tataatttga agaattgaaa
     2341 taaacaaatt tattgtgatg ataagtttta agtgcacaac tttggttatt tttcatttga
     2401 aatttttgga tagccagaaa aattgaacac gaaatgcatg ttgtttacat cgtttactgt
     2461 cttttacctt gataaaaatt aacggggaat ccctcaatta tcaatcctca accctcactc
     2521 ctcatccctc actcctcacc ttggctcctg ctggcttagg caatgaaaac tccccaggcc
     2581 ccagatcagg tcggtggaat cgatcccaat gacctcacgg tccggaaaac aggattggat
     2641 gatggacaaa gccagatcgt ctttggcgca gcggtaggta ggcacaatca ccgaacggtt
     2701 ggcgatgtag aaattggcat aagaagcggg caagcgctgg ccttcataat atagagggtc
     2761 cggcatcggc agttcgatga tttccagggg cttcccgttg aggaggcgca tggatttcaa
     2821 ctgccggaaa ttatgctcca ataaagcgtg gttttcgtcc tctttattgt tttcgcggac
     2881 gaccagaacc gcttcctcat tcacaaagcg gaccgtgtcg tcgatatgac catcggtatc
     2941 gtcccccaca atgccttcgt ccacccaaag gatctggtcg acgccataat acgcataaag
     3001 gtattcttca atttgtccct ggttgagctg ggggttccgg tttttattca acaggcaggc
     3061 ggtggaggtc accagggtgc ccttaccgtt aaaatccaca ctgccccctt ccatgacaat
     3121 attgggataa tagaccggca gcttcagttc caggcctacc caggtgggaa tttcatcgtc
     3181 caggtcataa ggtgggtatt tatttcccca ggcattgtat ccccaatcca cgatgatttt
     3241 gcctttttct ttatcaggat gaatgaggaa ggcgggtcca tggtctcggc accaggcatc
     3301 gttggtggga tggaaataaa aacggacctt ttccagatcg accccggcct tggtgagctc
     3361 gaccagggca aaatccttca tggcctggtc atttacattg atattcactt tttcgccccg
     3421 ggtcaactca aggataaact gcacataagg cgggtaaacg gtattcagtt tacccggcca
     3481 gctggcttcc ttatgcggcc aactcagcca ggtggattca tggggatgga attcggccgg
     3541 gaaataatac ccgagtttct ttggagtgac aatataatcc ggtattccgt gagggccggg
     3601 gccggcaggt ccgctgtgtt tattcatggc cttccggcat taaaggaatg tttgaggtat
     3661 tggatcatga gcaggagttc tacacatttc gcgtaaatat cctgaaaatc tttttcatct
     3721 gtgatggatc cctggaaata ccggcatcca tttttaaaag tgctttagaa aaccaaactc
     3781 ccgccaccgg gctttattca tccaggtacc gcttcaccag gtctccataa gaatcaattc
     3841 tgcggtctct cataaagggc caatgggtcc ggtaaaaatc ggttttatcc aggtcgaggg
     3901 cttcgacatg gatttcctcc tggtcatggg aagcctggta caggattctg ccaaaaggat
     3961 tggctatgaa ggatcccccc cagaacttca tggccccctc ttgttcaaat ccgacgcggt
     4021 tgacgctcac gacgtgaatt ccgttggcca ccgcgtggct tcgctgaatg gtttgccagc
     4081 cattgaactg ttcggcattg gtagcctcgt cctgggtggt ggcccagccg atggcggtgg
     4141 gataaaacag gatttcggca ccccttaaag cggtaagcct ggcggcttcg ggataccact
     4201 ggtcccagca aatcagaaca ccgatcctgg cgaaacgggt ttcaaatacc ctgtacccca
     4261 ggtctccagg ggtaaaataa aatttttcat aaaaggccgg gtcatccggg atatgcatct
     4321 tccggtattt ccccaggtag gccccgccgg catcgatgac ggcggtagtg ttgtgataga
     4381 tgccctgcgc ccgtttttca aacaaggaag caataatcac ggcgcccgtt tcacgcgcca
     4441 cctcctgcaa gcgctccgtt gacggaccgg ggacgggttc ggcgagcttg aaatgttcat
     4501 aatcttccgt atcgcagaaa tagagggagg tgaacaattc ctggaggcag atgatttggg
     4561 cgcccctgga agccgcttcg cgcaccaggc cgatggcttt ggccaggttg gtttccttat
     4621 cccgggtgca ggacatttga accagtccga cgttgacgat cttggtcatg gcagggtcta
     4681 agctttggac gaataattgg gtgattcctt ggtgatggtg atgtggtgcg gatggctttc
     4741 atgaatcccg gcagaggata ttttcaccat tttggcttgc tggagtgcct tgatgttggc
     4801 ggcgccgcaa taacccatgc cggcacgaag gccgccaata ttctgaatca tcacttctga
     4861 aagcaatcct ttgtagggta cccggccttc tatgccttcc ggcaccagtt ttttgatatc
     4921 gtcctcggcg tcctggaaat accggtcttt ggagcccgac tgcatggcat ccagcgagcc
     4981 catgcccctg taagttttga atttacggcc ttcataaagc acggtttccc ccggggcttc
     5041 ttccactccc gcaaacagac cacccgccat gatcgtgctg gcgccggccg cgatggcttt
     5101 cacgatgtcc ccggtaaacc ggatcccccc gtccccgata atgggaatgc cggaacctgc
     5161 tatgccccgg gatgcattca tgatggcgga taattggggc acgccgaccc cggctactac
     5221 ccgggtggtg cagatcgatc ccggcccaac ccctactttg accgcgttga ccccggcttc
     5281 gaccaaagcc ctggctccat ccgccgtggc cacattgccc ccgatgattt ccaggttggg
     5341 atgtcgttgc cggatcgttt tgatggcctc caatactccc ctcgaatggc cgtgggcggt
     5401 atcgatgcag atgacgtcca cctggacctg gaccagggca tcgacccggt cctgaagctc
     5461 tttattgacc cccaccgctg cgcctaccac caatcttcca aaagtatcct tgcaggaatg
     5521 gggataatct tccagccgca tgatgtcttt gtaggtaatg aggccgatca gtttgccggt
     5581 atcgtccacc accggcagtt tctccacttt atattgctgg agaatgcctt tggccagatc
     5641 gagggtggtg cccttggggg cggtgatcag cctttctttg gtcatgatct ccgtgatggg
     5701 ccggttgaaa ttggtctcaa aacggagatc ccggttggtg agtatcccga tcaggcggtg
     5761 gccttcatcg atgatgggga tccccccgat gccgtattta tccatgatca gtcgcgcatc
     5821 cctcacgcgg gcgccggctt tgagggtgat gggatcgatg atcatgccgc tttccgacct
     5881 ttttacgctt ttgacctggt cggcctgggc ttcgatgccc atgtttttgt ggatgatccc
     5941 gattccacct tcccgcgcaa tggctatggc cagctttttc tccgtgaccg tatccatagc
     6001 tgcggagacg atgggcacat tgagtttgat gcctgtggtg agttgggatt cggtattgac
     6061 ttccctggga agcacctggg aataggcggg gaccaacaag acgtcatcga aggtgtacat
     6121 ttcttcaatg accggactgg aggaggtagt cgttttttcc atgtgcaaaa ataagggaat
     6181 tgccctgatt cccgcaccgg ttccctggat tttcaatcag gaagtgaatg cagtcaatac
     6241 cactttaccg gattggacag ggtgccgata gtcccgatgc ggatgtccac caggtcatcc
     6301 tcttccaggg taaaggtggt tggagggaca atgcccgtac cggtcatgag gaaacagccc
     6361 tcattaaatt ccaaacctcg caagaggtaa tccctcagtt cttccggccg gcgcttcatg
     6421 gccccgagac gggtttgacc gtgaaacaca catttattgt ccctgaaaat tttaagatct
     6481 atttccgttt ccggacccag gggctcgccg ggaatggcca ggcagggccc aagcgcggcg
     6541 cttcgttcat aagtttttgc ctgggggagg tagagggtgt tttcgccttc gatcgaccgg
     6601 gaactcatat cgttgccaat ggtgtatccg gcaatggtgc cctgggagtt gataaaaagg
     6661 gtaagctccg gttcgggtac gttccaggtg gagtccctac ggatattcac ggcttgtccg
     6721 ggcccggcca cccggtgagg caatgatttg aaaaataatt ccggccggtc cgcgtcgtag
     6781 acgaggtcat aggccgactt ggcgtgttcg ctttcctcca tccgggcgtc ccggcttctg
     6841 agataggtga ccccggaggc ccagacttcc tgcgatccga tgggggccag gaccttgtcc
     6901 aggaacgcgg acgcggcgtc cttatccatg gcctggagtc cggcaatttc aattttaagt
     6961 tcttcaaaga gatttttccg gttaacccat tggtcccaat cgacggcggt ggaaaggaaa
     7021 tacctttcat tctcttcaat gataaaccgg ccgggggttt tataaagtct gaacatcatg
     7081 atcttatttt ttggaatttg tttctggttt ccagttttgc gcgatttcat ccagctcctc
     7141 gtaaaacgct tcgccgtatt tccggatgag ggcattcctt acaaaacgat agaccggcac
     7201 ccccaattgg tcgccgttgg tgcaagccgg agcgcaaatg gaccactggt cataattgag
     7261 agcctcccat ccgagcacct tatcttcccg gatgcggacc ggatagaggt ggcaggaaag
     7321 gggtttgatc cagtcggtct tgccaaagcg gtacgccgtt tcgagggcgc aggaaaggat
     7381 gcccgcccca tccctcaggg cataaatgca ggcgccatcc gcccggagcg gggtggcata
     7441 acggcccctt tcgagcttta catatcggtg cttttccaac agggtcctgg attcttcgtc
     7501 gagaaaaccg gccacctggc tgctgacccg gtccaggatg gctttctctt cttctgccag
     7561 gggagcgcct tcatcgcctt caatgcaaca agccccttta caggcctgca ggttgcagac
     7621 aaaatttttt tctaaaaggt cagtgctgac caacttgccc tggatttcca acatcggtcc
     7681 caaggtacga ccctatttcg aagtgtctct taccgggccc tgagcccgct taaaaaaata
     7741 ggaagcattt tgaaatttat tattttttta ggctttattc tgatattttt ggccttcaga
     7801 cttttaccgt attgaattga ttcacgccta gtattttaag gggaaatgga cgaactcaaa
     7861 agcatattca gggataaatc gcaacaactc aaagaaaaga taaaggccat gctcaaggaa
     7921 catggcaata tgaaaatcga tgaggtaagg ctggaacagg cattcggcgg aatgcggggc
     7981 attcaaagca tgatctggga aacttccctc ctggatgccc aggaaggcat caaattccgt
     8041 ggtttttcca ttccccagat ccaggaattg cttccaaaag ctcccggcgg ccatgaacct
     8101 ttgcctgagg gtattttctg gctgatgctc accggtgaaa taccgaccga tgcccaggtc
     8161 cagtggctca gcgacgaatg ggaacgccgt gcggtgatcc cccgggcgat gaccctggtc
     8221 ctcgatgcct tgccggaaga ggttcatccc atgacccagt tcagcattgc catcaacgcc
     8281 ctccaggccg actccatatt tgccaggcgt tacgaagaag gcatgcctaa aacggaatac
     8341 tgggacgcca cctacgaaga cgccatgaac ctcatcgccc gcattccgtt tgccgcagcc
     8401 catatctacc ggcggatttt taaagggaat aaacatattg cggccgaccg ctccctggat
     8461 tgggcggcca attattcgca catgctgggc ttcgagggcc aggaatgcca tgagatgatg
     8521 cggctttacc agaccattca cgcagaccac gaagggggga atgcctcagc gcataccgcc
     8581 cacctggtcg gttccactct gagcgatgtc tacctttcct atgcggcggc gatgaatgcc
     8641 ctggccggcc ccctgcacgg ccttgccaac caggaggtga tcaagtggat ttttgatatg
     8701 gtcgcggcgc tgggcacccg gaaacccacc agtgagcaga ttgcggctta tgtaaaagaa
     8761 actttgaata aaggtcaggt gatcccgggc tacggtcatg ccgtactccg tcaaccggac
     8821 ccgcgcttct atgcccagaa acggttttca gagaggtacc tggcggaaga cgatatcatt
     8881 cagatcgttt ggaggctgtt cgaaatcgtg ccgccgatcc tgcaaagtct tggcaaggtg
     8941 agcaatccct ggccaaacgt cgacgcccac agcggggcca tgctggtcca ctacggcctc
     9001 acggaataca attattatac tgtcctgttt ggcgtgtccc gctctttggg ggtcatggcc
     9061 gcccagtgtt gggcgagggc tctgggcttt ccgctcgaaa ggcccaaatc cctgacttca
     9121 gattgggtgt ttgattttgt ccagcacaac aatgatctca ataacgtcaa ttcttaacct
     9181 tgttaccatg tcaaaaatag aatccggatt aaaatacacc cccgaacacg aatggttcag
     9241 attggaatcc ggaaaccggg cggtcgtcgg catcacggat tttgcccagg gcgaactggg
     9301 tgaattggta tttgtcgaag tccctaccgt gggcaaaagc ctgaaaaaag gagacgtatt
     9361 cggcactgtg gaagcagtaa agaccacctc tgatcttttt atgcccattg gaggcaaagt
     9421 gctcgaattc aatgaagcca tcagcgaaag cggcggagac aatcccgggc tgatcaacga
     9481 agacccctac ggcagcggct ggatcatcaa aatggaagcc accgacctgt ctgaggtgga
     9541 aggcctgctc gacggggagg cttaccaaca gctgatccat tgaccggaaa ggacacacac
     9601 caaattgtct ttatactgca tcttgttttt taggaagtat ttgtattcgg ggatctggat
     9661 tttaatgatc ctggtgctca gtctgctgcc taaaagttct ttgcccgaaa tggagttcga
     9721 tatcccctat ctggacaaag tcgtccactt tgcaatgtat tttatcctgt tggttctcgt
     9781 atcctgggaa aggcaaaagc ttgaatctaa gccactgttt ttcaatggat tgatgcttgc
     9841 cttgctctta agtatctgta cagaggcggg ccagcggttt ttttttgcag gaaggagttt
     9901 tgagatattg gatattgttg ctaatattat cgggtcaatc agtggtattt attttttcag
     9961 aatcatttta aaaccgtaca aatgaatgtg accgagatag acggtagagt tgcattgatt
    10021 tttgtatttg ccatttttgt ggcagtggtg gcgatcatct ttatcctgaa atccgttttc
    10081 aggaaaaaag cgacggaaga tctgtcctcc aaatacaaag acaagaaatg ggcttcaccg
    10141 atcgagggta tgtacaaata cccggacgtc gatgtctttg ggatgagcgg gcctatctgg
    10201 cgcttcggac tcacagccgc actcgggctg gctgtcctgg gcctgaactg gaccacttat
    10261 gaccggaaaa tcgacatacc gacagacgcg ctggccttcg acgatgaaat tcaggtcgaa
    10321 ataccgcgta ctgcagaacc accccctcct cctccgccac ccccacctcc tgtagttcag
    10381 gagatcaagg tgaccgaaga aaaaatagaa gagaaggtgg aatttgtgga tcaatccatc
    10441 caggaagaaa ccgtagtgga agcgccgcct ccaccggagc ctaaaaaagc gcccccacca
    10501 cccccaccac cccctcctcc aaaagaagaa ccggacgaga tctttaaggt agtggaagaa
    10561 gcgccgcgct tcccgggttg tgaggacaaa gggtccacgg atgaaaagaa aaagtgcgca
    10621 gatgaaaaaa tgctgcaata cctgtataaa aacatcaagt accccaacat tgccaaagaa
    10681 aacggagtgc aaggtagggt ggtcgtcacc ttcgtcgttg aaaaagacgg acggatcacg
    10741 gatgcccgag tagtccgaga cattggcggc ggttgcgggg aagaagccct tcgcgtggtc
    10801 aattccatga acgaaatggg tgagcgctgg aaacccggca aacaaagggg caacccggtg
    10861 agggtccagt ttaacctgcc cgtgcaattc aaactcaatt aaaggtccag acgatctcaa
    10921 agcttatagg gccgcttggt tttaccagcg gcctttttat ttagattgta tattaaatta
    10981 tgatatttgc acctttcaga tttaaatcgc tgccatggcc gttcgaaaca taagtttcct
    11041 ctttagctgc tggctgattt ttttgttctc ttgcagaaac gaatccgcag gaacttcttc
    11101 cgctcctgaa acagattctt tggatagcat ttatcaactg gcccaggaaa agccaagatt
    11161 tcccggttgt gaagaccttc cgggcgaaat agccgcaaaa caaaaatgct cagaagagaa
    11221 actgcttcag tacctctata gtaaggtaaa atacccgcag tacgccctgg aaaacaatat
    11281 tgagggcagg tgtgtgatca gttttgttgt ggagaaagac ggatccatta gccagccgag
    11341 aatcgtaaag gatatcggcg ggggttgcgg ggaagaagcc atccgggtca tccaatccat
    11401 gaacgagatg ccggaacgct ggataccggg tcgccaggat ggtcttccgg tgcgggtaaa
    11461 gtttaacctc ccggtaaaat tccagcttaa ggaaagcgac gagcaagaag acgcccaata
    11521 aaatcagggg accgcgcccc ccatcctaca atcctattaa tcctattaat cctataatcc
    11581 tattaatcct accaatccta ccaatcctat caatcctacc aatcctatta atcctaccaa
    11641 tcctaccaat cctaccaatc ctattaatcc tactaatcct attactgccc caactgcatc
    11701 gtattatcca gcggaattct ctcttcctcc atagccaggt agaccgcgtc gagaagccgg
    11761 gtgcggtatt ccattttgtg caatttgttg ttttccatgg aagggtaggt ccggttgcac
    11821 aaaaacacaa agaccagttc ctggtcgggg tccacccatg ccgaagtacc cgtaaacccg
    11881 gtatgtccaa aggtattcgg agaacaaaat tgcccggcag gctgggtttt ttcggtattc
    11941 aattctttca tatcaaaacc aatccccctt ctcgtagaac cgagctggcg gctcgcaaac
    12001 tggctgatgg tggcgctccg gatcaggtga tccccgccgt accggccccc gtttaataac
    12061 atctgaaaca gggttcccaa ttcgtaagca ttgctgaaca atccggcatg accgcttacc
    12121 ccgtcgagca tggcggcgcc catgtcgtgc acatatcccc ggatcacccg gtttctccaa
    12181 taattgtcct tttccgtggg ggctattttt tgtaggggga aggattgata gggattatag
    12241 agggtgtggg tcagccccag gggtttataa aaatacttat tcacaaactg gtcgatccgc
    12301 atgccggtct ggtaacggat catctgggcg atcatgtaaa aacccaggtc gctgtaaaca
    12361 taattattgt tttctctcag gctggattcg agtatgcttt tccagatcag gtggtcgtag
    12421 cccgttttta gaaacatccc ctgggccacg ggcagactgt attttgaact gttggcggag
    12481 gcgtacaaag ccggatcggg tttgtacaca tgccgtttga cccggagcag cgtttctttg
    12541 taaaaaggga tccaggcttt aaaaccggcc tggtggctca tgatttccgt gagggtagcc
    12601 ctggatttgt tggtgccttt cagatcaggc aggtattcgg acatgggttg gtccaggtcg
    12661 attttccctt cgtcatacaa gcgcatgact gcgagggtgc tggcggcgac cttcgtgatg
    12721 ctggccagat cgtacaggtc atcggtccgg accgacgttt ttttttcgta atcgtgatat
    12781 ccgaaggctt tgtggtaaac gacctttcct ttccgggcca ccagcaactg gcctcccggc
    12841 gcggcatgga cgtttacaat ttcatggcag agactgtcca gggaaccgag gagcttatga
    12901 ttcaggccaa cccgctcggg tatggtgaaa ccaaaacgca tcaggctctc cgaatccaaa
    12961 cccgaaccgg cggggaaagc ggggccggaa ttaaccggaa gcctgccctt gcagccaatg
    13021 gcaccgaata tggcctgggc cgccaggtcc tgcatctggg gatcctcttc ataagcctgg
    13081 aggacccagg aagaagtttc aaaattcctt aaggcgtatg gacttccaaa ggaaacgagg
    13141 atgacccggc tttgctgttc cagcatttta ataaaattca gggtgccggg ggagaggcta
    13201 aaattttgtt tgagctgttt gctcaatcca tgaaggctca cgatcacgat atccttgcct
    13261 ttaagactgt taagcagggc agccgccttg gcttctggta tattgagttc cgtttgctga
    13321 tggctcacct tggcataact gtccattctc cattgaaagg ggcttttttc agtggctccg
    13381 atggacaccg aagcgagctt gtgctgatcc agttcggtga gcgggatcag tttgttttta
    13441 ttcgccacca gggtgagggc ctgttcgtac aatttacttt ttagcgcaat ggctttaggg
    13501 ctattgacct cgtcggtaag tttttccaag gataccggcg agtaggcatt caggcccatt
    13561 ttgtatttgg catgcaggat cttgcgtacc gcttcatcga gctgcagctt gaggggggcc
    13621 tcggcggcaa ccgctttttt cagttcctca aaagataagt cgatgctgtc cggcaggcaa
    13681 aggatatcgc atcccgcttt aaacgcaacg acttcgagcg ctccgggtgt gaaatttttg
    13741 gtgacccctt tcatttcgag agcgtctgaa aaaatcaggc cctggtaccc gtattcattt
    13801 ttgatcaggt ccgtcatggt ggacgaggac agggaagtcg gggtcctggg atccggttca
    13861 agggacggca cttgcaggtg ggctaccatc acgctttgca tgccctgctc aaaaagaagg
    13921 cggaagggga agagttcgat gctatccagc ctggcccggt tgtgattgat gaccggcagg
    13981 tcgaggtggc tgtccacatc ggtatcgcca tggccgggaa aatgtttggc gcaggccatg
    14041 acgcccatgt cctgcatgcc catcatgtac tggaaggatt ttgaggtgac cgccaccctg
    14101 ttttcaccaa aggaacggtc gttgatcacc gggttggcgg gattgacatt gatatccgcc
    14161 accggggcaa aattgacatg ggtaccgatg cgcttgagtt gccgggcaat ttccacaccc
    14221 atatcgtaga tcagtttatc ctcctggagc gccccgaggg taagttgccg gggaaaactg
    14281 atggcgcttt ccttaaaacg catgcccagg ccccattctg catcgatggc caccatcagg
    14341 ggtattttag acagggtttg atatttattg gtgagttcgg cctgtttgag cggagtgccc
    14401 tggaaaaaac acaggccgcc cacttgatat ttccggatct gcctttcgac ttcggcgata
    14461 tgctcggggc caaggttgga atgggcgcgg atcataaaca attggccgat tttctgatcg
    14521 gtcgtcaggc tgttgaatat ggaatccacc cacttctttt cctgaaacgc ggcctgctgc
    14581 ctcgcggtgt cagggctgga gtaagggagg ctgttgacct gtacgattaa aagaaaaaat
    14641 aagagtaagg ctttaatcat tttcaaattt attgaataaa caaagtacta tcaagcaaac
    14701 gggctttttc ataaaactcc cggcctttgg gggcgttgcc ggaattgaaa taagcagtgc
    14761 ctaaattaaa aagaactgcg gcatttccgg gttgaagggc aagggctttt tccaaaaatt
    14821 ccacggcccg ctgattgtcg cgattgaccc cgtgggccac tcccagcagt ctcagggtct
    14881 cgaaatcatc cggtttaaga ctgtaggcct gactcagata ttgtagggcg agatcggtcc
    14941 gtcccagctt ttccccctga cgtttgcctt cttcccggag ggcaaggtgc aggtttgctt
    15001 ttgcatgggt atgttcgggg tagagtgcca gggttcgtct ataggccagg atggattcat
    15061 cgaaccttcc ctggtaaaaa agggcgttgc ctttctgcag ccaggcgtca ctgtaaaggg
    15121 gatggatctg aagggctttc gtcagcctgc cctccgcctc cgcggcaagt tttatttttc
    15181 caaggctgtc tttttggctt gcggccaggg caaggagttc cccggccagg gcattgttga
    15241 ttttggcgct gttggcggac acccggatat cggtctggaa aagggtcaaa ttgtttttcc
    15301 aggcccttcc gcgcgacagt gttaaggctg ccgcaacgaa aatcagaaga agtataatcg
    15361 ccagggccgg cttattcaca gtctgaatga attggtagga ccaccccgcc actcccaggc
    15421 aaaagccgaa ggaagccatg tataaaaacc tttctgaaaa caggatgcca accgggaaaa
    15481 atacattgga ggtcagcagt agggaagtat aaatgaaaag gatgctgaag gaaagaaccg
    15541 gcatggattt tctgttgcgc caggccacaa cgaaggcacc caaatggatc aacagactca
    15601 gcaggacgga tggatccgac agtttcttca ggggaaaatg atttggaaaa taatcgtggg
    15661 tcagggggta ggggaggacc agcattttta aatcataagc cagggcgtaa aggatactgc
    15721 cccatttctc cgaagcggag cagaggatgt attgaccatc tgtgattttt aaaaaagggt
    15781 tgttcagcca ttccatggga agagggctgt ccaggatctg gcgaaacacc agggcccgga
    15841 cggagaggaa caggaaggcg ggaacgataa ataaactgat ttttctggtc gcagtcaccc
    15901 aatcctggcg gtccaggaag atataagcca gccatcccgc cggcaaccaa aggatggcgt
    15961 tttccttgga aaacaaggca agggtgaagc aaaccagaga cgcgcttccc cgaagcaggc
    16021 ccggccgtgg tttccaggag atccacagtg ttgccaggcc aaagagaaag gcctggattt
    16081 catccctccc tttaatattg gctaccactt ccgtgtggat gggatgcacc gcgtacaaca
    16141 gggcggccag tcccgcaatc cagtttgccc ggtcgggcga atagatacgg gccagggcag
    16201 tccttaaaaa gccaaagatg atcatgacgg tcaggccaaa cagcaggacg ttgatcaggt
    16261 ggtgcaacca cggcgctcct gataccgcat gttcaatggc aaaaaagacc aggctgaggg
    16321 gcctgtagcg gccaccggct acgaggtttt tgtcttctcc gaagaaaccc gtgaagctgt
    16381 cgtggctgag gatttccggg atcccccgga tccctttttg ggtaaactga ttctgagtga
    16441 ttaccagggc atcgtccagg acaaaatcat gcccgagggt aaggccataa caggccaggc
    16501 tgaggaagaa gcataccgta tacaccgttg aggaagacca gggaaaacgc gggtcccctg
    16561 caggagcgag ggcgcggatc ggatgatttt ccgttttatt cttcccgggc ttggccatcg
    16621 cacaaacata ttaaaaatta ttttaatatg ttcggatgca aacgttaaag gagccgggct
    16681 ttggttcctg gatgtaaaat ctctgattct gtgatgaatt gaacaggatt tggacaaatc
    16741 ctggtcttgc cggtggatat ggagaaaaaa gaactttgag gaggtgatcg gattacattc
    16801 caggaatatg gttaataaat tgattatttt ctccataggt ttattctact ccttagttgc
    16861 cggtcaaaga aaaccaaaac atgatgcaag ccactccagg ttccgtcgat aaaattgaac
    16921 accataaggc ccaggcctgg aaatggatcc tggaagcctg gaatcaaacc gcaacgagga
    16981 gctggtggct gatctttttg cccaggaagg ggaatttatg ggcatcccgc ctacccataa
    17041 acaggttcaa ttttcgggga tcgtcattca caggatagaa tatggaaaat ttgctgaaag
    17101 ccggaatgaa attgatctgt tgggtatccg tcaacaactt acctatgaat aattgcgtat
    17161 tatgaagact ccatggattt tattttgctc attgattcta ttgtcttgta tgcaacctca
    17221 aaaccaaaaa cccctggaaa cgcaagtttc caatatccgg tcggacagca tcaaacaatg
    17281 gatcaccagg tatctggaag aagtctggaa taaaagggat tttacgaatg ccgataaata
    17341 ctggggaccg gaatttgtaa atgttttcag tcctgaatcg gggaaaggcc cggaagcgat
    17401 gcgtcaacag gtagactatt ttctcaaagc ttttgaaaac agtcactttg aactgaagaa
    17461 catcctggtc cagggagacc aggtcgccct atggatagag atcagcggga agcacaccgg
    17521 cgaattgttt gggatcccgg caactcataa ggaagtccgg ttccgtgaag cagtttggta
    17581 taccctcaag aatggaaaat tgcatgaggt atatcccttt gtggactgga atgatttatt
    17641 ccggcaactg ggtaaatacc caaccctcga ctgagtgtta atttaaattc aaccccatca
    17701 aaatgaaaac aattatccgc ctatccgtcc ttcttttgtg gttttttaat gcctcgggcc
    17761 aggctccgct atctttaggc agcatcacca atttgtacga tgcttttgga aaagaaaggc
    17821 ctggagtagc ctttgatttt ggcttttcct gtatcattaa ttataacgga accatcatcc
    17881 tgtttgacgc gggttccaat gctgatactt ttgcccggaa tgtcaaagcc ctgggaatcg
    17941 acctggccag ggtggatctg gccgtggtgt cccattccca ttttgatcat ctcaacggca
    18001 tggactacct cctccaggta aatcccggtg tgaaaatcta ttatcccaat gatattttct
    18061 ggggagcgga tgttcctttt gatgccaccg gtcaggaacc ttccatcggg gattcattac
    18121 caaaggaaat gcggtatttc ggcggagggg ctacaaaatt ttctatcaaa caatccggcc
    18181 ggttctggga ggcaaatgta gaatatgtaa aaacctccaa agagatcgct cccgggatca
    18241 aactcattgc caccacttcc cccttcctgg gttatttcaa ccgctatccc aacctgagtt
    18301 ttgtacccgg ggtcttcaat gataacaata ataatcagtc aaaggccaat gaagccggtc
    18361 tgccggaact ttccctctcc ctggccactc cctccgggga gatcctgatc gtgggatgtt
    18421 cgcacagctc cgtggaaaaa atcaccgctg ctaccaggga atttacgaaa caatccatac
    18481 aattgcttta cggaggattt catcttctgc cctataaaag ggaggagatg tccggtatga
    18541 tccattacct gaaggacgaa ctaggagtcc gggaagtggc gcccgcccat tgcagcgggc
    18601 atctcgcttt taaaatgttc ggggacgctt atgggagcgg gttccacttt gcgggacttg
    18661 gggagaccgt aaggttttga ttttttcaca caaagcatcg ttcagggtcc cacaaaggcg
    18721 cggagagcac gaaaaaataa gtaaccgatt tatggattac aaggtcggag caaagcccct
    18781 cccaatcatt gggattcacc tggagtatct cgcacggagc gcacagagag cacggagatt
    18841 ttatcttggc ttacagcaaa accgtttatc tattcctctt gaatcttaga taatctttct
    18901 ccgtgccccc catgtgctca atgagaccct taaaagatct ctatgcgcaa agtgtacgtt
    18961 aagttggagt atctcgcacg gagtgcacag agagcacgga gatttatctt tggcttaaag
    19021 caaaccgcca ggcttttcct cttgaatctt aaatactcat tctccgtgcc ccccgtgtgc
    19081 tccgtgcgat ccataaaaga tctccgtgag agcaaagtag ttgacttaaa gcaaaaccgc
    19141 catgccaatc cgcttgaata ttaaatactc attctccgcg ccccccgtgc gctccgtgcg
    19201 atcaaaaaaa ctatctccgt gagaaataag agctgcccca aatccctcct accctcctta
    19261 accacgaatg attaacttag cgtgttaact tcgcactatt gatgatcaac gagctaagca
    19321 aacaactggc ccgcgaggcc atgaaacagg aaatcctctc cccacaggaa aaattgatgg
    19381 aatatgaaag ccaggaggat aaattgttca ttggcatcac ccgggaggag cagctcctgg
    19441 aaaaccgggt agcgcttacc cccaattccg tcatgatgct ggtgtccaat gggcaccggg
    19501 tgatcattga aaggggtgca ggcaaacgtt cgttttatac ggatgaggag tacgccgaag
    19561 ccggcgctca ggtgagtgaa gacaaaaagg aaatcctcaa agccagcatc attttgaagg
    19621 tggcccctcc cgggatcgag gaaatcaaca tgatgccggg tgaccaaatg ctgatttcac
    19681 ctcttcacat gcccctgctc accgaagaat atatccaggc cctgcgcacc cggaggatca
    19741 ccgcggttgc catggaatac ctcaaggaca aacacgacag ttttcccatc gtgcgcatac
    19801 tcagtgagat tgccgggctc agcgccatcc tgaccgcggc ggaattgttg tcggtcacca
    19861 gccacggacg gggcgtcctg cttggaggta ttgccggcat ccctccggcc aaggtactga
    19921 tcctcggcgc cggggtggtg ggtgaattcg ctaccaaggc cgccctgggc ctgggtgctt
    19981 ccgtacgcgt ttttgacaat aatatttata aactgatgcg ccttcaaaac agtgtcggcg
    20041 tgcccctgca tacctccgtc ctcaatccca aatacctgga ataccagttg cgcagcaccg
    20101 atgtggtgat cggggccatc cattccaaaa ccgggcgggc ccccatggtg gtatcggaag
    20161 acatggtcat gcgcatgaag cagggttccg tgatcgtgga cgtgagcatt gatcagggtg
    20221 gttgttttga aacctcggaa atcactaccc acgaacatcc cacctttatc aaacacgggg
    20281 tgatccatta ctgtgtaccc aatattgcct caaaggtgcc ccggacggct tcttccgcca
    20341 tcagcaatat cattgcgccg ctgctcctgt ccacctcgga agtgcgcaat atcgatgaaa
    20401 tgttatttgc caaccccggg ctccgtcatg gggtatatac ctacaaaggc catctcacca
    20461 atgaatacct gagccggcgg ttcaatatga agtttacgga tatcaatttg ctgttgactt
    20521 cgagttttta gttggcaggg tgacaagttg gtcagtcagc aagttggcaa gaggtaatgg
    20581 acccgatctt atcctttggg aaagccattt tttagggaag ttcacgagta gcctaaggag
    20641 ttgagactta ggaacatatt aaaat
//
LOCUS       DAOQXT010000086        20532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2967_length_20532_cov_6.143820, whole genome shotgun sequence.
ACCESSION   DAOQXT010000086 DAOQXT010000000
VERSION     DAOQXT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20532
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2967_length_20532_cov_6.143820"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..822)
                     /locus_tag="PKM27_14085"
     CDS             complement(142..822)
                     /locus_tag="PKM27_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08444.1"
                     /translation="MQTNPPKFNLLKVSRSQYIFDMYEREYLYFSLHKSFRGKNPEQK
                     GILDPREMNVSTWHPDYLSIIFKSTKQEFKFTKGKNIIDPEVNEAGDNDLNTLICSFY
                     WLNNELEDPNALYQRGFYIEGNQMLVINKPVEFCKTIYTSLRSLNLFFEGDHVKYYDP
                     KTWNGPRGSLTTHHKDIAFSYQNEFRIAIRPSNYGEPGILPSLPMEIPIPGLKQMSYI
                     NDIYPPIG"
     gene            complement(841..1032)
                     /locus_tag="PKM27_14090"
     CDS             complement(841..1032)
                     /locus_tag="PKM27_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08445.1"
                     /translation="MNISLRALEIFLLAQGKKRGYNEKQEVDVTTQGQRITLVPEGRA
                     IIITVDGIPKSMLKENGEV"
     gene            complement(1173..2414)
                     /locus_tag="PKM27_14095"
     CDS             complement(1173..2414)
                     /locus_tag="PKM27_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08446.1"
                     /translation="MPDYEKLKRLCEEASSISRTVIDDFLVYYAAFRDKQDREFDVRI
                     ERFRAMEKKMPAAWTPSVKMQYIGHRLFKQGGLIHKYLRSEDLKNREAEELNFLQELS
                     ASPWKFSFSVILDHPAPDFYEMEDVFRGEQYLLYSPAVTQTLSDHPVSLWFNLIFYNG
                     SCWQTYGPLTGLQSYGPDDIFFYATELNESIDSDEELLEDVEKNPVPYMMLMSGSTHP
                     ILLQSGYEAVLIKGEAPLTGFDFQTMKNDFRIQFDSGVFKLSHKTLSDPPHLAEVYYD
                     EKESVLHASAMTDYGYHELIGCLNGYGIDFPIEPDIRIHPSLLDLIRKLLNKKVNLFP
                     YNHLFEEKKSPESEEFLAKLNQVLSMALPEINAGRKPDAEALARKLDLDPETVRDILD
                     NVIGRVDKLKKDGPFGHGPEA"
     gene            complement(2465..4714)
                     /locus_tag="PKM27_14100"
     CDS             complement(2465..4714)
                     /locus_tag="PKM27_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNR08447.1"
                     /translation="MKPSLLTINILILVLLFNGIVTTSSLSAQSKVNIAVDQRPPEAE
                     NLNVLQDWLRWNNPGSFRTHFFNSLASCCFDARDQEIARLFTRKDWLARQEIIKEKLL
                     EAIGPFPEKTPLNPRITGVIEKEGYRIEKIVFESHPGFYVPGCLFIPDGIKGKAPAVL
                     NVFGHEQESYHKALDQVIILNLVKKGMIVFAIDPLSQGEHVQYYDPEIKFSSIGYSVV
                     EHSYFGNVCFLAGSSGAKYFTWDGIRAIDYLVSRPEVDPERIGVTGFSGGGTVTAYLG
                     AFDPRVKAMVPCSWSTYNRRLLEMKGTQDAESILTHGLKNGLSFEDLIEVRAPKPCLL
                     TFTSRDEYLSLQGAREAYQEAAGAYAALGQPDHLQMVEDDSKHWVTPKIREEIYRFFM
                     KHFDLSGEAAEEEVALLSGQDLQVTPTGQIATSFGGDRVYEVNKKESLPLIEKLEQSR
                     KEISTHLEKVRKKAMEVSGYVAPCCPGAEPVINGRYQREGYTVTKFAIPGEGEYAIPL
                     LLFVPDSLPGKRSTIVYLHPSGKAAEAKAGGEIESLVKKGYLVAAADVLGTGETANTA
                     SRGQTDPYTGVLVGRSLVGIQAGDIVRVVNYLLQRPDVDSNGIGAVARGELAIPLLHA
                     AAFDPTIRSVTLISSLISYRSVVMNRIYKIGLTARPGGGHHHPYEVDFAWGIAGVLKA
                     YDLPDLMGCIAPRRLVVADPRNQVLENPTTAELNLDLEFPLEVYRVKKAGEKLRIIEN
                     MDRLEFIMD"
     gene            5881..6972
                     /locus_tag="PKM27_14105"
     CDS             5881..6972
                     /locus_tag="PKM27_14105"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNR08448.1"
                     /translation="MKKKGSLFIGSYSGIKVYIHWTFLILIGWILMSHFQAGKDLGGA
                     LSGVAFILLLFLCVVLHEFGHALTAKRFGIKTHDISLYPIGGIASLEKLPEKPGQELL
                     VALAGPMVNMVIAIFLWIYLTSIGGFPGTEEVNSAEYWQNMPMVNKLFIANITLAVFN
                     LIPAFPMDGGRVLRALLAFKMDRTKATRIAAGIGQGLAVLFVFLGFFYNFMLVFIGLF
                     IYLGAGGEALQETVKNAMSHFIVRDAMMTRFTPVAATDSLDSVSKLVLESQDKVFLVA
                     DQTQIHGVLTSNELIKGLSQFGTNASVAQVMRQDYLTLTSSMPLQEAYEKLSLSPCQV
                     APVVDGQQLLGILDKENIHELLMIRQKLA"
     gene            complement(7624..8667)
                     /locus_tag="PKM27_14110"
     CDS             complement(7624..8667)
                     /locus_tag="PKM27_14110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR08449.1"
                     /translation="MFPPPLRAICFLCGCLTSLQNFGQSDRILPDSLVLGWMNEFNIP
                     ASGIGYLQEGKILHTKVLGELQEGKPAPQNTLFQVASLTKPIVEMTVLRLVSQGLWSL
                     DEPLYPYWVDPEVKDDPRHLKLTTRHVLSHQTGFVNWRWLHPSKRLTFDFEPGTRTQY
                     SGEGLEYLKKAMEARFKMPLQEIVTKYLFFPDGMTDTHFFWDEGSEESRYAVAHDKDA
                     KPYEIRKNREASAADLLMTTVMDYSRFALGVLQKKQLSPEVWQEMIRIGAPDQNSKFG
                     LGWEIYTGLPGGEYILLHTGSDPGVRTLVALLPNSQAGLIVFTNGDNGMKLIIKVVEE
                     ALEIGAELVKRGG"
     gene            complement(8723..10099)
                     /locus_tag="PKM27_14115"
     CDS             complement(8723..10099)
                     /locus_tag="PKM27_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR08450.1"
                     /translation="MQAQTLRPDLRNPSIWTASGIHAEPLDDVKYPGIRFNETTQGGL
                     YILNQPEFSEGSIEFDLRGRNQLQKSFVGLAFHIQNDSVYDAIYFRPFNFRPEDTSRQ
                     SRAVQYISSPKNHWFTLRTAFPGKYENKVQPVPDPDGWFHVRIEVAGQEVKVYVNNSE
                     MPSLSVTKLSSTTSGRLGLWVGNQSDAGFANLEISPFKKMNAVPYGNNKDAGSYCQVG
                     DARIYYEVYGQGSPIVLLHGGVYGYIDEFKPLIERLAAHFQVICIGTRGHGKSEMGRL
                     PFTWAQRANDAYQVIRAVSNEKTTVIGFSDGAYTAYKLAALYPQAVNKIVAIGAGDFP
                     KNSKREKFNYNPELLMRDAANFFKSRLALMPEPNRWDECLQGLNHLYNNETLSKETFQ
                     QITCPTLLVAGDQDQYHRPEDLVNVARYIPESQLCIINGCDHVVFYCNFPMFWESIRT
                     FINSGKLP"
     gene            complement(10360..10938)
                     /locus_tag="PKM27_14120"
     CDS             complement(10360..10938)
                     /locus_tag="PKM27_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08451.1"
                     /translation="MKLLSSIFIILLGLSAKGQNPSYFYQIPAYPESYTAGTVAGRMV
                     DGLGFRYYWATEGLTEKDLAFKPGDSARTSLETLEHILSLTQILVHAVNQKPTVSQAP
                     APKMDFHQIREKTLMNLEEASKILKSDRAVLDSFPMIFQNPTRTTEFPFWNLINGPIA
                     DALWHVGQVVSFRRSSGNPFPSGVSVLQGVKR"
     gene            11108..12427
                     /locus_tag="PKM27_14125"
     CDS             11108..12427
                     /locus_tag="PKM27_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08452.1"
                     /translation="MSEEAEKEIIESCLLEIESRLGWGGSADWTHYDFEKLSEILEKG
                     TGIQLSVTTLKRLWGKIKYDHAPSLTTLNTLASFLGYEDWRGFKLSLKDSTPGVATEL
                     LEEVILSAPLEKPLRENRTVRQGKSVFFLFSFGIIALIGLFALNLKKAHSSSPYSIDP
                     ARFSFEADKIKTSGLPNSVIFRYDASASPSDSVFISQTWDIRRKTPVDKYKKNHSAIY
                     YYPGYFKAKLMVDTTVVKTHDIQITTDGWLGLVETDEGTPIYYQEADLLKAGWVGLEA
                     SALEARQLPLLPVSPKVRIYNQRDFEGLMNDRFVFETQVMNPFRDGDNACQRVQVLIH
                     CKDDIISIPLSAKACVGDLQLSFAGARVHSKSADLSGFGADLNQWTHLKVVSLNKKAT
                     LWVNGVEAYSLEFPNEPTEIVGVQYRFNGPGLIRDTWFLQDGRKIEL"
     gene            12477..13625
                     /locus_tag="PKM27_14130"
     CDS             12477..13625
                     /locus_tag="PKM27_14130"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HNR08453.1"
                     /translation="MKNRRREFLKKTAALTASMPLIQSGRANPVLGMEVPKRRAWIKD
                     AGIQLCLAYFWGIEPRKTALARQMAVYGAVGGINPAMAGLQGKSSIDREVIKGVKAAW
                     QKEGLQLKVIEGPPSLGTKTKLGLEGRDEEIGQFITLMKNLSAEGIDTICYNWMPVVN
                     WARTTLDKPGRGGALVSSFDLDDARSQTPITEYGELSHDGMWKNLEYFLKAAVPEAEK
                     YGIKLALHPDDPPVDRLRGIPRIMTSVEAFKKLLDLYPSPSNGLTFCQGSFASMGEEG
                     KGEDIPAAIEYFGKRNAIHFVHFRDVRGYKNKFEETFHDDGKTNMFKAMQTYLRMGFK
                     GPMRPDHVPTMAGDSNEHPGYSTIGTLFAIGYIRGLIEGAAKGDMKSY"
     gene            14098..15060
                     /locus_tag="PKM27_14135"
     CDS             14098..15060
                     /locus_tag="PKM27_14135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNR08454.1"
                     /translation="MVFLDFEKPLEDLFEELSKLQKIQEKKEVDVSEPIREMEQKIKN
                     TRKEIYSNLNGWQKVQVSRHPERPYTLYYIEQICKTFIELHGDRNIRDDKAMVGGLGI
                     IDNQSVVIIGHQKGINTKMRQYRNFGMANPEGYRKALRLMKLAERFGFPVITFIDTPG
                     AYHGPEAEERGQAEAIARNLFEMARLRVPILCYVIGEGASGGALGIGLGDKVFMLENT
                     WYTVISPESCSSILWRSWDYKQQAAEALKLTAEHMLEFGLIDDIVKEPLGGAHADPET
                     MAKTLKKHIKKELAEIMDEKPETRIKKRINKYTKMGQFKKGTPA"
     gene            15065..15625
                     /locus_tag="PKM27_14140"
     CDS             15065..15625
                     /locus_tag="PKM27_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08455.1"
                     /translation="MKSPQPFLYSFRLARFRNKLIREAESRILDKKPVHELAIRHMAI
                     LFDASSLEDKNEVLRFAKLFDKLRIQVHLLAYLDYKAGTSGLPFRHFNRGDVNFFFIP
                     DNSDIRTFLDFKYDLLINADLSQNLSIHYLAVLAYAPFKVGPKSQFDEYYHLILDTSD
                     TFSIKQYISELILILNKVSFHGHLVY"
     gene            15606..16493
                     /gene="dapA"
                     /locus_tag="PKM27_14145"
     CDS             15606..16493
                     /gene="dapA"
                     /locus_tag="PKM27_14145"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNR08456.1"
                     /translation="MDTSFIKGLGTALVTPFAGDRVDAEALKKIVANNLAHGVDFLVP
                     LGTTGETSTLTDSECHLVINTVLEANEGRVPVVCGWFGKNNTRQVLEMISAFRFDGIS
                     AILVASPYYNKPSQAGLFQHFIAIAEASPVPVLLYNVPSRTAVNITAETTLKLAEHPN
                     IIGTKEASGDFNQIMQVLKHKPSSFRVLSGDDLVTLPMIACGAEGVISVISNAFPGIF
                     AAMVDSALEGNFNDAKFHNDLLLDVHKWLYVEGNPVGIKAALQILGFCADEVRLPLWP
                     LSSPHKQQLREAIRHLSPL"
     gene            complement(16471..16584)
                     /locus_tag="PKM27_14150"
     CDS             complement(16471..16584)
                     /locus_tag="PKM27_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNR08457.1"
                     /translation="MNGEELSTAEDFNKKNAEQQASERALAKLGIKEATDA"
     assembly_gap    16600..16609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16610..17209)
                     /locus_tag="PKM27_14155"
     CDS             complement(<16610..17209)
                     /locus_tag="PKM27_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HNR08458.1"
                     /translation="MLRKLYNFYISDDKDFAKRIKSMTGVTPKKLNLYKLAFYHRSTF
                     NEQSEMAQNNERLEYLGDALLNSISAEYLYHKYPGANEGFLTKMRSKIVKRKTLNYIA
                     DAMGLDTLLMEFNKTHISVSMLGNALEALIGAMYLELGYEPTKKFVVNQLFRKFLDIH
                     ELEINDDNFKSRLLEWGQKKGHEVQYRVISKFKMDHRDRY"
     gene            complement(17220..18461)
                     /gene="fabF"
                     /locus_tag="PKM27_14160"
     CDS             complement(17220..18461)
                     /gene="fabF"
                     /locus_tag="PKM27_14160"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNR08459.1"
                     /translation="MRRVVVTGMGALTPIGNHVGAYLEGLQQGKSGAAPITHFDASLF
                     KTRFACEVKDLHVEELIDRKEAKRMDRFAHFAMISTAEAIRDSGLDLNQVDMDRAGVI
                     WGSGIGGLQSLEAEIMDYARGSGTPRFNPFLIPRMISDIAAGQISIHYGFRGTNYGTV
                     SACASSNHAILNSCDQIHYGKADLMISGGSEAAIIATGVGGFNAMKALSERNDDYTTA
                     SRPFDKDRDGFVLGEGAGCLVLEEYEHAKKRGARIYAEIVGGGASADAYHITAPHPEG
                     KGALSVMRTALRDAGLDPSSIDYINVHGTSTPLGDISEIKAIQAVFGDHAFKMNISST
                     KSMTGHLLGAAGAIEGIACILAIQHDFIPPTINHFTDDPEFDPRLNLTFNEAQHRRIN
                     FALSNTFGFGGHNTSVIFKRV"
     gene            complement(18643..18882)
                     /locus_tag="PKM27_14165"
     CDS             complement(18643..18882)
                     /locus_tag="PKM27_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNR08460.1"
                     /translation="MASIEEKVKKIIVDKLGVDEAEVTAEASFTNDLGADSLDTVELI
                     MEFEKEFDISIPDEQAEKIQTVGQAISYLESQINK"
     gene            19096..>20532
                     /gene="pyk"
                     /locus_tag="PKM27_14170"
     CDS             19096..>20532
                     /gene="pyk"
                     /locus_tag="PKM27_14170"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNR08461.1"
                     /translation="MSDFTLLNTKIIATVGPAISSFEMLLAMAKAGVKVFRLNMSHSD
                     QEAHLEVIRHLTRINEEYHFHVGILADLQGPKLRIGKIKDQAIDLQTGDEVIFTNTPC
                     EGTADGIYMSYELFPKEVAAGDKVLVDDGKLIFEVVDTDRKNSVRLKVLFGGILSSNK
                     GVNLPDTLISLPSLTDKDLKDLDFLIGQPVHWIALSFVRKADDIHDLRERLQKAGHQA
                     RIVAKIEKPEALKNINEVISATDAVMVARGDLGVEVPIEKLPGIQKEIVRRCIEHSRP
                     VIVATQMMESMIQNPSPTRAEVTDVANAVLDGADAVMLSAETSVGKHPIRVVEAMSRI
                     IAETERHYQPNQNRLPKLSKDSETFLADVLCYNAVKTSVDLDAKAIIGMTTSGFTAFK
                     ISSFRPKAEIFIFSSVAHMLSTLNLVWGVQCFEYHYDHISTDELMNKVLQTLKNKNLL
                     SRGDIVVNTGTVPVQKEGRTNMMKISVVE"
BASE COUNT         4952 a         5159 c         5185 g         5226 t
ORIGIN      
        1 agaatgccgc cctgtcacgg cggaggtcgc gggttcgagt cccgtccggt ccgcatcggt
       61 ttttaattca agtccctgat aatgttattg ttagggactt ttttgtttga acaatttgct
      121 ccagtgggcc aatatggata cctatccaat tggaggataa atatcattta tgtagctcat
      181 ttgtttcaat cccgggatag gaatttccat cggcaaggaa ggcaagatgc caggttctcc
      241 gtaattggat ggccttattg ctattcgaaa ttcattctga taagagaatg ctatatcctt
      301 atgatgggtg gtcaaacttc ccctaggtcc attccaagtt ttgggatcat aatatttcac
      361 atgatcccct tcgaaaaata aatttaaaga cctaagtgaa gtgtaaattg ttttacaaaa
      421 ttctactggt ttattgatta ctaacatttg attcccttca atgtaaaatc ctctttgata
      481 caatgcattt ggatcttcca attcgttgtt caaccaataa aatgaacaga ttaatgtatt
      541 tagatcattg tcccctgcct cattaacctc aggatcaatt atatttttgc cctttgtgaa
      601 tttaaattct tgttttgttg acttaaatat aattgacagg tagtcgggat gccatgtact
      661 tacgttcatt tctctaggat ccagaatacc tttttgctct ggattctttc cacgaaaaga
      721 cttgtgcaat gaaaagtaga gatactctct ttcgtacata tcaaatatgt actgggatct
      781 gctgaccttc aataaattga attttggtgg gttagtttgc attaatgcta agttattcaa
      841 ttaaacttct ccattctcct tcagcatact cttcggaatc ccgtcaaccg ttatgatgat
      901 ggccctccct tccggcacga gagtgatccg ctgcccctgg gtagtaacgt ccacctcctg
      961 tttctcattg tagccgcgtt tctttccctg ggcgagaagg aagatttcca gggcacgcaa
     1021 ggatatgttc attcgttgac ttcatcaggg tatgcaatcc ttgttcggcc acggccaggt
     1081 tatcttcatg gatatcttcc aattcatcct ggaatttttg cggccggtcg ctgacactct
     1141 gcctggagtg ctggttcccg aatttgatgc catcaggcct caggaccatg cccaaacgga
     1201 ccgtccttct taagtttatc aacccggcct ataacattat caagaatgtc cctgaccgtc
     1261 tccgggtcca ggtccagttt tcgggccagc gcctcggcat ccggttttct gccggcattg
     1321 atttctggca gggccattga taggacctgg ttcagtttag cgaggaattc ttcactctcc
     1381 ggtgattttt tttcctcgaa taaatggttg tatggaaaaa gattcacttt tttattcagc
     1441 aattttcgga tcagatcgag cagggaggga tggatgcgga tatcgggttc tatggggaag
     1501 tcgatgccgt atccgttcag gcatccgatg agttcatggt aaccatagtc cgtcatggct
     1561 gaggcgtgca ggacgctctc cttctcatca taatagactt ccgccaaatg cggtgggtca
     1621 gaaagtgttt tatggcttaa tttaaatacg ccggaatcga attggatccg gaaatcattc
     1681 ttcatagtct gaaaatcaaa gccggtcagc ggcgcttccc cttttattaa gacggcctcg
     1741 tatccgcttt gcagaaggat gggatgggtc gaaccgctca tcagcatcat atagggcacc
     1801 gggttttttt caacatcctc cagcagttcc tcatccgagt caatggactc gttcaattcc
     1861 gtggcataaa agaaaatatc gtcagggcca taactttgca aaccggtaag cgggccatag
     1921 gtttgccagc aggaaccatt ataaaagatc aggttgaacc acagggatac cgggtggtct
     1981 gacaaggtct gggtgacggc tggcgaataa agcagatact gctctccccg gaatacatct
     2041 tccatctcgt aaaaatccgg cgcgggatgg tccaggatca cactgaaact gaatttccag
     2101 gggcttgcgg acaattcctg taaaaaattc agttcttcgg cttccctgtt tttaagatcc
     2161 tcggaccgta aatatttatg aatcagccct ccctgtttaa aaagacggtg tccaatgtat
     2221 tgcattttga ccgaaggggt ccaggcggca ggcatttttt tctccattgc gcggaaccgt
     2281 tcaatccgaa catcaaattc acggtcctgt ttgtcccgga atgcagcgta ataaaccaga
     2341 aagtcatcga tcaccgttct ggaaatactg cttgcttcct cgcagagccg cttcagcttt
     2401 tcgtaatccg gcatggttcc atttgaatga aaaggtatga gaaattgtcc gtatcgggaa
     2461 ttgcctaatc cattataaat tccaggcgat ccatattctc gatgattctc agtttctccc
     2521 cggccttctt aacgcgatac acctccaagg gaaattcaag atcaaggttg agttccgcgg
     2581 tggtcggatt ttccaacacc tggttcctgg gatcggcaac caccagcctt ctcggcgcaa
     2641 tacagcccat cagatccggc aagtcatagg ctttcaaaac gccggcaatg ccccaggcaa
     2701 aatcgacttc ataaggatgg tggtggccgc ctccgggcct ggcggtcaac ccgattttat
     2761 agatccggtt catgactacg gaccggtaag agatcaggga acttatcagc gtgaccgagc
     2821 ggatagtagg gtcgaaagcg gcagcatgaa gaagtggaat tgccagttcc ccgcgtgcta
     2881 cggcgccaat cccattcgaa tccacgtccg ggcgttgtag gagataattc accaccctta
     2941 cgatatctcc cgcctgaatc cctaccaggc tgcgtccgac cagtacgccg gtatagggat
     3001 cggtctggcc tcggctggct gtattggcgg tttctcccgt cccgaggaca tctgcggccg
     3061 cgacgaggta tccttttttt accagggatt cgatttctcc accggctttt gcttcggcgg
     3121 ccttgcctga aggatgcaga taaacgatcg tgctgcgctt acccggcagg ctgtccggaa
     3181 caaacagcaa taggggaatg gcgtattcgc cttcacccgg gatagcaaac ttcgtcacgg
     3241 tatatccttc ccgctgatac ctcccattga tgaccggttc agccccgggg caacaaggcg
     3301 ccacataacc ggaaacttcc atcgcttttt tccggacttt ctccaggtgg gtgctaatct
     3361 cttttcttga ttgctccagt ttttcgatca gcggcaggct ttctttttta ttaacttcat
     3421 acacccggtc ccctccaaaa gaagtggcga tctggccggt tggggtcacc tgcaggtcct
     3481 gccctgaaag aagtgccact tcctcttccg cagcttcgcc ggacagatcg aaatgtttca
     3541 taaaaaaacg atagatctct tccctgattt tgggagtcac ccagtgtttg gaatcgtcct
     3601 ccaccatttg aagatgatcc ggctgaccga gcgcggcata agccccggcc gcctcctggt
     3661 aagcttcccg cgcgccctgc aggctcaggt attcgtcgcg tgaggtgaaa gtcaacaggc
     3721 atggcttggg ggccctcacc tcgatcaggt cttcaaagct caacccattc ttaagtccat
     3781 gggtcaatat cgattctgca tcctgggtgc ctttcatttc cagcagcctc cggttgtagg
     3841 tggaccagct gcagggtacc atcgctttta cccgtggatc aaaggctccc aggtaggcag
     3901 tgactgtacc accccccgaa aagcccgtta ccccgatcct ttccgggtcc acttccggtc
     3961 gggagaccag gtaatcaatg gccctgatcc catcccaggt aaaatattta gcgcccgatg
     4021 atccggctaa gaagcagaca ttcccgaaat aggaatgttc caccacactg tagccaatgg
     4081 aggaaaattt gatttccgga tcataatact ggacatgttc tccctggctg agcggatcga
     4141 tggcgaatac gatcatccct tttttcacca ggttaaggat gatgacctgg tccagggctt
     4201 tatggtagga ttcctgttca tggccgaaga cattgaggac agccggggct ttccctttga
     4261 tcccgtcggg aataaagagg catcccggta cataaaatcc cggatgggat tcgaacacga
     4321 ttttttcgat gcggtatcct tccttttcaa ttactccggt gatcctggga ttcaaggggg
     4381 tcttttctgg aaagggtccg atggcctcca ggagtttttc ttttattatc tcttgccggg
     4441 ccagccagtc cttccgggta aacaatcggg cgatctcctg gtccctggcg tcaaaacaac
     4501 atgaagcaag gctgttgaaa aaatgggtcc tgaaacttcc cggattgttc cagcgcaacc
     4561 agtcctgcaa gacattcaga ttttcggctt ccggcggtct ttggtcaact gcaatattta
     4621 ccttggattg ggcagacagg gatgatgtgg tgactatgcc gttgaagagc agaaccaata
     4681 ttaaaatatt tatggtaagg agggaaggct tcatgacttt aatataatgc aaaacgggtc
     4741 gaagtggtag ctgtgagggt agtgattttt aagccgttgt atatccatcc cggccttcag
     4801 gccggggtta aggtaaaggg aatttatggg ctttagcccc agatgaaagt gatagggtga
     4861 gtctatatgg tgcttcactg tgctgggctg aagcccaatt ggcgctccgc gtttaaacct
     4921 caggctaaag cctgaggttg gatgatcagc ctccccagta actactttcg ttgatggatc
     4981 aagcatattg ccctgatggg tagagatttt aagccgttgt atatccatcc ccggccttca
     5041 ggccggggtt gatgcataag aagagtatgg gctttagccc cagatgaagg tgataggtgt
     5101 ttctacatgg tgcatatgct tccctgtgct gggctgaagc caaattggcg ctccgccttt
     5161 aaacctcagg ctaaagccag aggttggatg atcagcctcc cctgtaacta ctttcggtgg
     5221 tgaatcaacc atatagccct gatggatata gatttttaag ttgttgatac catccccggc
     5281 cttcaggctg gggttgaggt aaagggaatt tatgggcttt agccccagat gaaggtgata
     5341 gttgagacta catggtgcat atacttcttt gtgctgggct gaagtccaat tcacgctatg
     5401 cctttgaacc ccaggcttaa gcctggggtt agatgatcaa cctcccctgt aaccactttc
     5461 ggtgatgaat caaacatatt gccctgatgg gtaaagattt taagccgttg tatatccatc
     5521 cccggccttc aggccggggt tagtgtaaaa gaagattatg ggctttagcc ccagatgaaa
     5581 gtgataagtg agtctaccat gttgcatatg cttccctgtg ctgggctgaa gcacaattca
     5641 cgctatgcct ttgaacccca ggcttaagcc tgggggttag atgatcaacc tcccaagtaa
     5701 ctaatggcgg tgatggatca atcacatcac ccaaatgggt ggagatttta agccgttgat
     5761 atccaacccc ggccttcagg ccggggttga ggcaaaggga atttatgggc tttagcccgc
     5821 ttcttcttaa tttgtactgg caaaacgttg agcccttcgg gcttttaaaa actcattctt
     5881 atgaaaaaaa agggttcctt attcatcggt tcctactcag gcatcaaagt atacatccac
     5941 tggacctttc tcatcctgat cggctggatc ctgatgtccc atttccaggc gggcaaggac
     6001 ctgggcggcg ccctcagcgg ggtggccttc atcctccttc tttttctctg cgtggtcctg
     6061 catgaattcg ggcatgcgct cacggcaaaa cggtttggca ttaaaaccca cgatatttcg
     6121 ctttatccga tcggtgggat cgccagcctg gaaaaattgc cggaaaagcc gggccaggaa
     6181 ttgctggtag ccctggcagg gcctatggtg aatatggtca tcgcgatttt tctttggatc
     6241 tacctgactt ccatcggagg ctttccagga actgaagagg tcaattcagc ggaatattgg
     6301 cagaatatgc ccatggtgaa caaactgttt attgccaata tcaccctggc tgtctttaac
     6361 cttataccgg cattcccgat ggatgggggc cgggtgctca gggcccttct ggcatttaaa
     6421 atggaccgga ccaaggccac ccggatcgcg gcgggaatag ggcagggcct ggcggtgctt
     6481 tttgtcttcc tgggtttctt ctacaatttt atgctcgtgt tcatcggctt gtttatttac
     6541 ctcggcgccg gcggcgaagc gctccaggaa acggtgaaaa acgccatgag ccattttatc
     6601 gtccgcgatg ccatgatgac ccgtttcacc ccggtggcgg ccaccgacag cctggattcg
     6661 gtttcaaagc tggtgctgga aagccaggat aaagtattcc tcgtcgccga tcagactcag
     6721 atccatgggg tcctcacctc caacgaactc attaagggcc tctcccaatt tggcaccaac
     6781 gcttccgtgg cccaggtgat gcgccaggat tacctcacct tgacttcctc catgcccctc
     6841 caggaagcct atgaaaaatt atccctcagc ccctgccagg tagccccggt ggtggatggc
     6901 caacaacttt taggtattct ggacaaggag aatattcatg agttgctgat gatcaggcag
     6961 aagttggcgt gaaaaaatgg tgggtggtgg atggtgggtg gtgggtggtg gacctgaccc
     7021 ttggcatttt tgaaaatatt caattgactt atccctgtac tcattcagac aagggtaagg
     7081 ccgggtgttt ttctatttag attctgccag aatccttggt tcccccgtgg gcctgccccc
     7141 aggcgggatc tgattattgg tgatatgggg ggacaggggt gttgattata ttgatataga
     7201 attctttttt ttaattgtgt tccgacccct gaccccttga aggggaaccc ggcgcttgat
     7261 atttcggaat aaatttaatg gacccacctc tgtacttatt caggtaagtg cagggccgaa
     7321 agttatactt taagatgctg ccaggatctt tggtaccccc gtgggcctgc ccccaggcgg
     7381 gatctgaata ttggtgatat tgggggggac aggggcgttg attatattga tatagaattc
     7441 tttttttaat tgtgttccga cccctgaccc cggaagggaa cctgaccctt ggtattggaa
     7501 caaatttaat ggacccatct ctgtgcttat tctggcatga gcaaggaaga ttgttttatt
     7561 taagttgact gccaggatcc ttgatccctc aatcctcaat cctcaaccct cattcctcaa
     7621 atctcacccc cctctcttca ccaattccgc cccaatctcc aacgcttcct cgacgacctt
     7681 gatgatcaac ttcatcccgt tatctccatt ggtaaagacg ataaggccgg cctggctatt
     7741 tggtaacaag gctaccaggg tacggacacc aggatcgctg ccggtatgga gcaggatata
     7801 ttccccgccg gggaggccgg tgtaaatttc ccagcccagc ccgaatttgg aattttggtc
     7861 cggggcccca atgcggatca tttcctgcca tacctcaggg ctaagctgct ttttctgcaa
     7921 tacccctaag gcgaaccggg aataatccat caccgtggtc atcagaagat cggccgcact
     7981 ggcttcccgg ttttttctga tttcataggg tttggcgtct ttgtcgtggg ctaccgcata
     8041 cctggattcc tctgagcctt catcccagaa aaaatgagta tccgtcatgc catcagggaa
     8101 gaacagatat tttgtcacga tttcctggag gggcattttg aatctggctt ccatggcctt
     8161 ctttaaatat tcaagccctt cgccggaata ttgggtgcgg gttccgggct caaaatcaaa
     8221 agtcagccgc ttgctcggat gcagccagcg ccagttgaca aaacctgttt ggtgtgaaag
     8281 cacatgccgg gtggtcagtt tgaggtgcct ggggtcatcc tttacctccg ggtcgaccca
     8341 gtaagggtac aagggttcat ccaaagacca aagcccctgg ctgaccagcc tcagtactgt
     8401 catttccacg atgggtttgg tcagggaagc cacctggaac aaagtgtttt gaggcgccgg
     8461 ttttccttcc tgcaattcac ccagcacttt ggtgtgcagg atttttcctt cctgaagata
     8521 tccaatgccg gatgcaggta tattgaattc attcatccag ccaagaacca ggctgtcggg
     8581 caagatccga tcggattgtc caaaattctg aagagaggtt aaacatccgc acaggaaaca
     8641 gatggcccgg aggggtggag gaaacatact actaaagact aagggccggc ggagaggttg
     8701 cacctcgagc aagatcagaa ggtcagggca atttaccgga attgataaaa gtccttatgc
     8761 tttcccaaaa catgggaaag ttgcaataga acaccacatg gtcgcaaccg ttgatgatgc
     8821 aaagctggct ttccgggata taccgcgcga cgttgaccag gtcttccggc ctgtggtatt
     8881 ggtcctggtc cccggcaacc aagagcgtgg gacaggtgat ttgctggaag gtttctttac
     8941 tcagggtttc gttattgtac aaatggttaa gtccctgcag gcattcatcc cagcggttgg
     9001 gctcgggcat cagggccagc ctgcttttga aaaaattcgc agcatcccgc atcaacaact
     9061 ccgggttgta attaaatttt tccctcttgc tgttctttgg gaaatccccg gcgccgatgg
     9121 ccactatttt atttacggcc tgggggtata aggctgccag tttataggcg gtgtaagcgc
     9181 catcgctgaa cccaatgacc gtggtttttt cattgcttac cgcacggatg acctggtaag
     9241 catcgtttgc cctctgggcc caggtaaagg gcaggcgccc catttccgat ttgccgtgtc
     9301 cgcgcgtacc gatgcagatg acctggaaat gcgcagccag tcgttcgatc agcggcttaa
     9361 attcatcgat gtacccatag acccctccgt ggagcaggac gatggggctg ccctggccgt
     9421 acacttcata ataaatccgg gcatccccga cctgacaata agatcccgca tccttgttgt
     9481 tgccataagg gacggcattc atttttttaa aaggactgat ctccaggttg gcaaagccgg
     9541 catccgattg attgcccacc caaaggccca gtcttcccga agtggtggaa ctcagttttg
     9601 taaccgaaag gctcggcatt tcactgttat tgacatagac ctttacctcc tgtccggcga
     9661 cctcaatccg cacatggaac cagccgtcgg gatcgggcac gggttgcact ttattttcat
     9721 atttccccgg gaaagccgtt cgcagcgtaa accagtgatt cttgggtgaa gaaatgtact
     9781 gcactgccct ggactgccgg ctggtgtctt cagggcgaaa attaaacggc ctgaagtaaa
     9841 tggcatcgta cacgctgtcg ttctggatat gaaaggccag gcctacaaaa cttttctgca
     9901 actggtttct gccgcggagg tcaaattcaa tgctgccttc ggaaaattca ggctgattca
     9961 ggatgtacag gcctccctgc gtggtctcat tgaaccggat acccggatat ttgacatcat
    10021 ccaagggttc cgcgtgaata cctgaagcag tccagatgga agggtttctg aggtccggac
    10081 gaagggtttg ggcctgcatt gtccaggacg ttaaaaggaa taaataaaat gcatttttca
    10141 taattgatta ttgtgggttt gtattaaggt ttgaggtttg aggttagagg ttagagcttt
    10201 gggagttccc agcttccagc ttccaggtta gaagctcgaa gctagaagtt agaagctagg
    10261 gcgtaatggc ctggcagtta cgggaagttt ggtagtccca cccctagctt ccagcttcca
    10321 acttcgagct acgagctacg agctacgagc ttacagctcc tacctcttaa ccccctgcaa
    10381 aacactgaca cccgacggga acggatttcc ggaagatctc cggaagctca ccacctgtcc
    10441 gacatgccag agtgcatcgg caatggggcc gttgatcagg ttccagaagg ggaattccgt
    10501 cgttcgtgtt ggattttgaa agatcatggg gaaggaatcg agtacggccc gatccgattt
    10561 caggatttta ctggcctcct ccaggttcat caaagtcttt tcccttatct ggtggaaatc
    10621 cattttgggc gcaggggcct gggataccgt tggtttttga tttaccgcat ggaccaggat
    10681 ttgggtcagg gataaaatat gttccagggt ttccagggaa gttctggcgc tgtcgccggg
    10741 tttgaaggcc aggtcttttt cagtgagtcc ctctgtggcc cagtaataac ggaatccaag
    10801 accatcgacc atacggccgg cgaccgtgcc cgccgtgtag gattccgggt atgcgggtat
    10861 ttgatagaaa taagatggat tttgaccctt ggcggaaagg cccaggagaa tgataaagat
    10921 tgaggaaagc agtttcatgt cggctttgaa attttttaca aatattattg gaggggcctt
    10981 gaatacggtt tccaaaccgg tgacaagttg gtccaatttt attgcaaacc ggcaagggca
    11041 ggagataagc ctgtttttta acttatcctt aaactatttt tcatcaattt agatcaacga
    11101 aatagccgtg tcagaagaag ccgaaaaaga gattatagaa tcctgcctcc ttgaaataga
    11161 gtcgaggctg ggctggggcg gcagcgcaga ttggacgcat tatgattttg aaaaactcag
    11221 cgaaatcctg gaaaaaggga ccgggataca gttgagcgtc accacgctta aaagactctg
    11281 gggtaaaatc aaatacgatc atgccccctc cctcaccacc ctgaataccc tggcttcctt
    11341 cctgggttat gaagactggc gcgggttcaa attgagcctg aaggatagca ctccaggggt
    11401 agctacggaa cttttggaag aggtgatcct gagcgcgccg ctggaaaaac ctttaagaga
    11461 gaatcggacc gtcaggcagg gtaaatccgt gttttttttg ttttccttcg ggatcatcgc
    11521 cctgatcggc ctgtttgccc tgaacctcaa aaaggcccat tcttcctcac cctattctat
    11581 agatcctgcc cggttttctt ttgaagccga taaaatcaaa acctccggtc tgcccaattc
    11641 ggtgattttc cggtacgacg catccgcctc cccatccgac tctgtattta tttcacagac
    11701 ctgggatatc cgtcgtaaaa cgccggtcga taagtataaa aagaaccatt ccgccatcta
    11761 ttattacccc ggctatttta aagcaaaact gatggtggat accacggtgg tgaaaacgca
    11821 tgatatccag atcaccaccg acggctggct cggactggtc gaaaccgacg agggaactcc
    11881 aatttattac caggaagcgg acctgttgaa agcgggatgg gtagggctgg aggcttcggc
    11941 cctggaggcg cgccaacttc cgctgctgcc ggtcagtccc aaggttcgga tttataatca
    12001 aagggatttc gaaggcctga tgaacgaccg gtttgttttt gagacccagg tgatgaaccc
    12061 cttccgggac ggagataatg cctgtcagag ggtgcaggta cttattcatt gcaaggacga
    12121 catcatcagt attcccctct ccgccaaagc ctgcgtgggg gatttgcaac tgagtttcgc
    12181 gggggcccgg gtccacagca agtctgccga tctctccggc ttcggcgctg atctgaacca
    12241 atggacccac cttaaagtgg tctctttgaa taaaaaagct accctttggg tgaatggggt
    12301 ggaagcctat tccctggaat tccccaatga gcccaccgag attgtcggag tgcagtaccg
    12361 ctttaacggc ccggggctca tccgggatac ctggttcttg caagatgggc gcaagatcga
    12421 attgtaaaca gtgaaggggt gactggcagt caccccttca cttaattata tacttcatga
    12481 aaaaccggag aagagaattt ctgaaaaaaa ccgcggcgct gaccgcatcc atgcccctga
    12541 tccagtccgg ccgggcaaac ccggtgctag ggatggaagt cccgaaacgc agggcctgga
    12601 tcaaagatgc aggcattcaa ctctgccttg cctatttctg gggaatagag ccgcgtaaaa
    12661 ccgccctcgc caggcaaatg gccgtttacg gcgcggtggg cggcatcaat cccgccatgg
    12721 ccggcttgca aggcaaaagc agcattgacc gggaggtgat caaaggggta aaagccgcct
    12781 ggcaaaagga gggcctgcag ctgaaggtga tcgaaggtcc tccctccctc ggcacaaaaa
    12841 ccaaacttgg cctggagggc cgcgatgaag agatcgggca attcatcacc ctcatgaaaa
    12901 acctttccgc cgaaggcatc gatacgatct gttataattg gatgcccgtg gtcaattggg
    12961 ccaggaccac cctggataaa ccgggcaggg gaggggccct ggtgagttcc tttgatcttg
    13021 acgatgcccg cagccagacc ccaatcaccg agtatgggga attgagccac gatgggatgt
    13081 ggaaaaacct ggaatatttt ctgaaagcgg cggtgcccga agctgaaaaa tacgggatca
    13141 aactcgccct ccatccggat gacccgccgg tggaccgcct cagagggatc ccgcggatca
    13201 tgacctctgt ggaggctttc aaaaaactgc tggaccttta tccgagcccc agcaacgggc
    13261 tcaccttctg ccagggcagt tttgcgtcca tgggtgaaga aggcaagggg gaagacatcc
    13321 cggcggccat tgagtatttc ggaaaaagga atgccatcca ctttgttcat ttccgggatg
    13381 tccggggata taagaataaa ttcgaggaga cttttcacga cgatggcaaa acaaacatgt
    13441 tcaaagccat gcaaacctat ctccggatgg gattcaaagg cccgatgagg cccgatcatg
    13501 tgccgaccat ggccggggat tccaatgaac atcccgggta cagcaccatc ggaacgcttt
    13561 ttgccattgg ttatatccgg ggtttgatcg aaggggcggc caaaggcgac atgaagtcct
    13621 attgacctgt tcatggagtt tttttaagtc aggccggtct gtatccgtgc tattttttat
    13681 tccaggcact caggcatatt gccgggcttc tgcacccaaa ggttgaccaa aaaacggaat
    13741 agagggaggt tagaggttgt ttttggacct ccagggccaa gctccgtgaa ctccaattct
    13801 ccgtgcgcct tcaaagtgat gggttgacta ttggtcatct atccgtttcg ccaaatggat
    13861 aaactctttg ataaattctc cgtgcgcgaa gattgcgaat cttccaaatc aaaaggggtg
    13921 gttgatggat agtatccttg ccttttaaat tgagaccttg atgtaaattg aacatctccc
    13981 aataaataca ccggggtaga ggataagcct tgtacatgat tgaaattcag gtaaataggg
    14041 cacatagacc ggatattgaa atttttctac ctttgcgcaa attagaatcg tcgccttatg
    14101 gttttcttag atttcgaaaa gcctttagaa gacctgtttg aagaactatc caaacttcag
    14161 aaaatccagg aaaaaaagga agtggatgtt tcagagccca tccgggaaat ggaacagaaa
    14221 atcaagaaca cacgcaagga aatatattcc aatctcaacg gatggcagaa agtccaggtg
    14281 tccaggcacc ccgaaaggcc ctatacccta tattacatcg aacagatctg caaaaccttt
    14341 attgaactgc acggtgaccg caacatccgc gacgacaaag ccatggtggg cgggctgggg
    14401 atcatcgaca atcaaagtgt ggtgatcatt ggccatcaga aagggatcaa caccaaaatg
    14461 cgccaatacc gcaatttcgg tatggccaat ccggaagggt accgcaaggc gcttcgcctg
    14521 atgaaactgg ctgaacggtt tggttttccg gtgatcacct tcatcgatac gccgggcgct
    14581 tatcacgggc ccgaagccga ggaaaggggg caggccgaag ccatcgcgcg caacctgttt
    14641 gagatggcca ggctcagggt gccgattctc tgttatgtga tcggcgaagg cgcttccggc
    14701 ggcgccctcg gaattgggct cggcgacaaa gtgtttatgc tggagaatac ctggtatacc
    14761 gttatctcac cggagtcctg ttcttccatt ctctggcgta gctgggatta taaacaacag
    14821 gccgcggaag cgcttaaact caccgcagaa cacatgctgg aattcgggct gatcgacgac
    14881 atcgtgaagg agcccctggg cggagcccat gccgatccgg agaccatggc caaaacgctg
    14941 aaaaaacaca tcaaaaagga attggccgag atcatggacg aaaaaccgga aacgaggatc
    15001 aaaaaaagaa taaacaaata cacgaagatg ggccaattta aaaaagggac gcccgcctga
    15061 ccgtatgaaa tcccctcaac cctttttata ctctttccgg ctggcccggt tcaggaataa
    15121 acttatccgg gaggctgaaa gccgaatatt agacaaaaag cccgtccacg aactggcgat
    15181 ccggcatatg gccatcttgt ttgatgcctc aagcctggaa gataaaaacg aagtgcttcg
    15241 ttttgcgaaa ttgtttgata agctccggat acaggtgcat ctcctggcct acctggacta
    15301 caaggccggc acctcaggcc ttcctttccg ccatttcaac cgcggggatg tcaatttttt
    15361 tttcatcccg gacaacagcg acatcaggac tttcctcgat ttcaaatacg atctgctgat
    15421 caatgcggac ctcagccaga acctctcgat ccactacctt gctgtcctcg cttatgcccc
    15481 ctttaaagtg gggcccaaat cccaatttga cgagtattac cacctcatcc ttgacaccag
    15541 cgataccttc agcataaaac aatatattag cgaactcatt ttgatactca ataaagtcag
    15601 ttttcatgga cacctcgttt attaagggat tggggaccgc actggtcacc ccctttgccg
    15661 gtgaccgggt ggatgcggaa gcccttaaaa aaatagtggc gaataacctg gcacatgggg
    15721 tggattttct cgttcccctg ggcacgaccg gtgaaacctc aaccctgacc gattcggaat
    15781 gccacctggt gatcaatacc gtgctggaag cgaatgaagg aagggtgccg gtggtttgcg
    15841 gttggttcgg aaaaaacaat acgaggcagg tcctggaaat gatcagcgcc ttccggtttg
    15901 atgggatcag cgccatcctg gtcgccagcc cgtattataa taaacccagt caggccggcc
    15961 tgttccagca tttcatcgcc atcgcggagg caagcccggt ccccgtccta ttgtataatg
    16021 tgccttcccg tacggcggtc aacatcaccg cggaaaccac cctcaaactg gcggagcatc
    16081 ccaatatcat agggaccaag gaggcctccg gggatttcaa ccagatcatg caggtattaa
    16141 aacacaaacc ctcaagcttc agggtccttt ccggcgatga cctggtcacc ctgcccatga
    16201 tcgcctgcgg ggctgaaggg gtgatttcag tgatcagcaa cgcctttccg ggcatctttg
    16261 ccgcgatggt ggattcggcc ctggaaggga attttaacga tgcaaaattt cacaacgacc
    16321 tgctcctgga cgtgcacaaa tggttgtatg tcgaagggaa cccggtgggg atcaaggcgg
    16381 ccctgcagat cctgggcttt tgcgcggatg aggtgaggtt gcccctctgg ccattaagca
    16441 gccctcacaa acagcagctc cgcgaggcca tcaggcatct gtcgcctctt taatacccaa
    16501 tttcgccagg gctctttccg aagcctgttg ctccgcattc tttttgttga aatcctccgc
    16561 ggtggaaagt tcttctccat tcacggtgac gaccacccgn nnnnnnnnng tagcggtcgc
    16621 ggtggtccat cttgaatttt gaaatcaccc ggtactggac ttcatgccct tttttctgcc
    16681 cccattccag caaacggctt ttgaaattgt cgtcgttgat ttccagttca tggatatcca
    16741 ggaatttccg gaaaagctgg ttgacaacaa attttttagt cggttcataa cccagttcga
    16801 ggtacatggc cccgatcagg gcttcgaggg cattgccgag catggaaacc gaaatatggg
    16861 ttttgttgaa ttccatgagc agggtatcca ggcccatcgc gtcggcgatg taattcaggg
    16921 tttttcgctt tacgattttt gacctcatct tggtcaggaa cccttcattg gctcccgggt
    16981 atttatggta gaggtattcc gcggaaatgc tgttgagcag ggcgtcgcca aggtattcca
    17041 gccgctcatt gttctgggcc atttcggatt gctcattaaa agtgctgcgg tggtagaagg
    17101 caagtttata caagttgagc ttcttcggcg tgacgcccgt catgcttttg atacgcttgg
    17161 caaagtcttt atcgtcagag atatagaaat tatacagttt acgcagcaca gactttgtat
    17221 taaaccctct taaagataac agaagtattg tgtccgccaa aaccaaaggt attgctcagg
    17281 gcaaagttga tccggcggtg ctgggcctcg ttgaaagtca ggttgagccg gggatcaaat
    17341 tcgggatcat cggtaaaatg attgatggtc ggggggataa aatcgtgctg gatggccagg
    17401 atgcaggcaa tgccttcgat cgcgcccgct gcccccagga gatggcccgt catcgatttg
    17461 gtggaactga tattcatctt gaaggcatgg tctccaaata ccgcctggat ggccttgatt
    17521 tccgaaatat ccccgagcgg ggtcgaggtg ccgtggacat tgatgtaatc gatcgaagac
    17581 gggtccaggc ccgcatcccg cagggcggtg cgcatcacgc tgagcgcccc cttaccttcc
    17641 ggatgcggtg cggtgatgtg gtaggcgtcg gcggaagcgc cgcccccgac gatttcggca
    17701 tagatccggg cgcccctttt ttttgcgtgt tcgtattctt ccaggaccag gcaacctgcg
    17761 ccttctccca gtacaaatcc atcccggtcc ttgtcaaaag gccgggaggc ggtggtataa
    17821 tcgtcgtttc gttcagacag ggctttcatg gcattgaagc cgcccactcc ggtcgcaatg
    17881 atggccgctt cagagccccc gctgatcatc aggtccgctt tgccgtaatg gatctgatcg
    17941 caggaattca ggatggcatg gttgctggag gcgcaggccg aaaccgtgcc gtaattggtg
    18001 cccctgaatc cgtagtgtat ggatatctgc ccggcggcaa tatctgagat catccttgga
    18061 atgaggaaag gattgaagcg gggtgtcccg ctgcccctgg cataatccat gatctccgct
    18121 tccaaagact gcaaaccgcc gatgcccgaa ccccagatca ccccggcccg gtccatatcg
    18181 acctggttga gatcgaggcc gctgtcgcgg atggcttccg ccgtggagat catggcaaag
    18241 tgtgcaaacc ggtccatgcg tttcgcttcc ttccggtcga tcaactcttc cacatgaagg
    18301 tccttgactt cgcaggcaaa acgcgttttg aaaagggaag cgtcaaaatg agtgatgggt
    18361 gccgccccgc tttttccctg ctgaagccct tcaagatagg ccccgacgtg gtttccaatc
    18421 ggggtcaggg cgcccatacc ggtgactaca actcttctca tattgatatt gctgatggtg
    18481 gttcgttcag gattgcaaat ttacaaaagt ttcccgcgac ccaaaggctt ccgcggggtt
    18541 cataacggat ggatcgcaaa atccgtgatg atctgatggg aaatacaaat aaaaaaagtc
    18601 cgcatgggga tatgcggact ttatattaaa aactgtaaat gtttatttat tgatctggga
    18661 ctccaggtaa ctgatggcct gtcccaccgt ttgaattttt tcagcctgct catcggggat
    18721 cgaaatgtca aattctttct cgaattccat gatcagttcc acggtatcga gcgaatcagc
    18781 acccagatca ttggtaaaac tggcttcggc ggtcacttcc gcttcatcaa ccccaagctt
    18841 atcaacgatg atttttttta ctttttcttc aatgcttgcc atttgttaga aggtttaaaa
    18901 atttctgcaa aaataaaatt tagtttttga tgataaaatt ttttttaaaa ccggacccgg
    18961 ccgtttgcag aaccgaacct tcccgttttg taggtgataa tatcattttg acttcaaaag
    19021 tttcacaaaa tagaaatatc ttttaccccc ttttccagat attttgctgt atctatgcgc
    19081 aaaaaaaatt aaagtatgag cgattttacg ctcctcaata ccaagatcat agccaccgta
    19141 ggccccgcca tttcctcctt tgagatgctg ctggccatgg ccaaagcggg ggttaaggta
    19201 ttccggctca acatgtcgca ttccgatcag gaagcccatt tggaagtcat ccggcacctg
    19261 acccgcatca acgaagaata tcatttccat gttggaatcc tcgccgacct ccagggtccc
    19321 aaactccgta tcggcaaaat caaggaccaa gccatcgacc tccagaccgg cgacgaagtc
    19381 atatttacca ataccccctg cgaagggacg gcggatggca tctacatgtc ttatgaactt
    19441 tttccgaaag aagtggccgc gggtgataaa gtactggtgg atgacgggaa actgatcttc
    19501 gaagtggtgg acaccgaccg caaaaattcc gtacgcctta aggttttgtt cggcggcatc
    19561 ctttcatcca ataaaggagt gaacctgccg gataccctga tctcccttcc ttccctgacc
    19621 gacaaagacc tcaaggacct ggatttcctc attgggcaac ccgtacactg gatcgccctt
    19681 tctttcgtgc gtaaagcgga cgatatccac gatttgcggg aacgccttca aaaagccggg
    19741 caccaggcgc ggatcgtagc taaaattgaa aaaccggaag cccttaaaaa tattaatgag
    19801 gtgatcagtg ccaccgacgc cgtaatggtc gcccgcgggg acctcggagt ggaagtgccc
    19861 attgaaaagc tcccgggtat ccagaaggaa atcgtcaggc gctgcattga acacagccgt
    19921 ccggtgatcg tcgccaccca gatgatggaa agcatgatcc agaatccaag ccctacccgt
    19981 gcggaagtga ccgatgtggc caatgccgtt ttggatggag cagacgccgt catgctgagc
    20041 gcggagacct ccgtcggcaa acatcccatc cgcgtagtgg aagccatgtc gagaatcata
    20101 gcggaaacgg aaaggcatta ccagcccaac caaaaccgtc tgcccaaact gtctaaagac
    20161 tcggaaacct tcctggctga tgtgctttgc tacaacgccg tgaaaacctc agtcgatctc
    20221 gatgccaaag ccatcatcgg catgaccacc tccggtttta ccgcttttaa aatatccagt
    20281 ttccggccca aagccgaaat atttattttc tcttcggtag cccatatgct gtccaccctc
    20341 aacctcgtct ggggcgtcca gtgttttgaa taccattacg accatatcag taccgatgaa
    20401 ctgatgaata aagtcctgca aacccttaaa aacaaaaacc tcctctccag gggggatatc
    20461 gtggtcaata ccggtacggt gcccgtgcag aaagaagggc ggacgaatat gatgaagatc
    20521 tcggtggtgg ag
//
LOCUS       DAOQXT010000087        20484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_2980_length_20484_cov_5.806354, whole genome shotgun sequence.
ACCESSION   DAOQXT010000087 DAOQXT010000000
VERSION     DAOQXT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20484
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2980_length_20484_cov_5.806354"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="PKM27_14175"
     CDS             <1..1498
                     /locus_tag="PKM27_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR08462.1"
                     /translation="QENAKTNRNWSADYINTWQRVYEPKVYEGGYPRIVIVPNPDGTV
                     TATFDRAAASKWIADNQNLLTQWHRENNKLADKGNPINNSLDRFEPGTARYDQALDEI
                     RNTYNNEGGTRFFDQSALYHVHGEYKFTPKFVNTWTVGANARLYAPESRGTIFYDSAG
                     LKITNFEYGVYTGMEKKTNDDRWTFSGTIRLDKNENFDFLMSPAASVVFKPRANNFLR
                     LSFASAIRNPTLSDQYLHLNVGPAILAGNIDGVQNLITVSSFLDYVNTLKTDQLVYFN
                     IDPIQPEKVKTFELGYRTTLFNKLYADLGYYYSIYNDFLGYNIGIDATFDPVSGFPQR
                     LQAYRYAANSINQVTTQGAAAGLNYYFGTYYQIQGNYSWNRLNKEFEDDPIIPAFNTP
                     EHKYNIGLSGRDIPLKVGGLRLNDFGFNINYKWIDSFQFEGSPQFTGFIPSYNLLDGQ
                     MNWRIRPWNTTVKIGASNLLDNKQFQAYGGPRIGRLAYLTLLYEWKKN"
     gene            1551..3161
                     /locus_tag="PKM27_14180"
     CDS             1551..3161
                     /locus_tag="PKM27_14180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08463.1"
                     /translation="MKQARFVSLLLMAVWTFTAYGQGIRYLHPVFDSVSVSNTFYGMN
                     VTILSVPTTGKGTRVPLPVSIYSPTGDTVKNRPLVIYIHTGNFLPFPQNQGTAGRTVN
                     GGALAALGMGFPVDSPLVEICTRLAKMGYVVAAIDYRTGWNPVASTQTERVNTLINAA
                     YRGVQDFRTAVRFFKKTFKEAGNPYGIDTSKIVAWGQGSGGYVSLASSVLDNYLDIPL
                     ASKGKFIGPDITGDGFGDPYVLPPVHGDIYGTSLGINPTNGDTLSLPNHVGYSSDFQL
                     SVNMGGALPDTLWLDATDLPLISFQEPRDPFAPYKEGLVLVPRGTMAPLPVVEVQGSY
                     LAVEKARRLGLQKKMIDLKLNDVYTQAANVKNDGIEGLYPISGNPLNPLDSDPWNWWD
                     TAFMASVERQAGTGNNTNGLLTNPDMSAAKARRYIDTIIGYYAPRAFAVLNLGTFVNT
                     PDLLKDTDVGFKLMPNPMVNQTLLQTNPDFPMKSVTLYDLNGRVVRKLSGLNTNSITV
                     HRNELSQGAYILQVGFKKGVISKTLLIQ"
     gene            complement(3313..4470)
                     /locus_tag="PKM27_14185"
     CDS             complement(3313..4470)
                     /locus_tag="PKM27_14185"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNR08464.1"
                     /translation="MKSTTLGRRDWLKAGLGLALGSAFPLSLQAETKQYLNRVHPHTG
                     RWMLEEAPVALKARLTSNENPYGPSDRAKMAIIHGFKDANLYSRTVAIELKSKIAAYE
                     GVRPEQVFLCAGLSELLNLVGAVTGLEHGDLVSAHPTFDLMLTTASAIGGQWIKVPLN
                     KKFEHDLGAMEKAITAKTKLVYICNPGNPCSTIVDPEELSAFCSRVSKKTLVFVDEAY
                     NDYIPDAARHSMVSRLRAGDQVMIGKTLSKIHGFAGLRVAYALATTGFIKELEKYRTW
                     DFTMNAPSMHGALATLQDEEFKQYCVMKNREARELAVKGLIALGYEPLPSYTNFLIFP
                     IQKEGKSFVQVMQNQGIGLRSWYFNDKHWCRVSVGTSEEMQLFLDALKQVG"
     gene            4720..5379
                     /locus_tag="PKM27_14190"
     CDS             4720..5379
                     /locus_tag="PKM27_14190"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR08465.1"
                     /translation="MKNLLIIILILSSNLAMAQGSWTTLFDGKSLEGWHNFKKTTVSG
                     WMIEDGVLTTDGKGGDLVSDKEFGDFILEFDFKVSPKGNSGIVYKVVEGEGEAYFATY
                     ASGPEYQLIDDVNYPSKLNETQKTGANYDLNPPKSMPSRPAGEWNSGSITVKKNKITH
                     KVNGKTVVKYTYGDEAWKAAVAKSKFAKWPYANPHHRGKIALQGHNDRVYFRNIRIKE
                     L"
     gene            5412..6608
                     /locus_tag="PKM27_14195"
     CDS             5412..6608
                     /locus_tag="PKM27_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08466.1"
                     /translation="MQKVAMLGSGFIGRFYAKAIHNQRSRDRVVAIYARREESAKKFA
                     DDYGCPIWSTDMETVIAHPEVTMVCIALPNNLHESAVHLCAKHRKSVICTKPLGRNSE
                     EAYRMLKAVEEAGIFAGYLEDLCYTPKNLKAVEMARNGSLGRILWAKSRETHPGPHSD
                     WFWDKEQAGGGCMLDLGCHCVEIGRNYIGKDIKPIRAFCWAATQVKPIDAEDHGIALI
                     EYENGAIGQFETSWSFRGGMDLRDEVMGTEGTIWLNNFLRTGFEAFTTGKGGDYIAEK
                     SETNSGWLFPVGDEVNELGYNHMFTDMFSAAEEGREPAETFYDGYIVNAILDACYRSA
                     ESGKWEPVELKEWRRRTGLSKFSGYQEYDADHYFIKEEVTHDGRHKLILKDKKSGKFV
                     EKDIKG"
     assembly_gap    6836..6935
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7060..7338
                     /locus_tag="PKM27_14200"
     CDS             7060..7338
                     /locus_tag="PKM27_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08467.1"
                     /translation="MDSILPILINAIFGGGGGWLASMLKKNGLGLVGNLLSGAVGGNV
                     LPLITSALGLFANSGDGGSLNLTSILTALVGGGAGSLLGGLFKKAPAA"
     gene            7497..8177
                     /locus_tag="PKM27_14205"
     CDS             7497..8177
                     /locus_tag="PKM27_14205"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNR08468.1"
                     /translation="MKTFYTIASALFIAVNAFAQQGLELSIGPSFTNVSVRGLQSSLR
                     PDRTLHTGLQVQALWDQDLGSGFSMATGLGYKEKGFHVREGIDMDVFNLPVSLGVEAI
                     TTLKYLELPLQWRYHTQGDGPLGFFVFAGPALSYALEGEVRTRANLIFDVNLTKTKLN
                     LDQDIYNRMEWSGLAGVGGTLRLKSGALTVQAGYQHGFSRVVDNTLIDLRLRNYGFGM
                     NIGYRINL"
     gene            8334..8492
                     /locus_tag="PKM27_14210"
     CDS             8334..8492
                     /locus_tag="PKM27_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08469.1"
                     /translation="MNQTKSESSRLMWVYTLISLVIMLFLLAVKPEWFWVALPFFGTY
                     LVKAFGVI"
     gene            complement(8504..9226)
                     /locus_tag="PKM27_14215"
     CDS             complement(8504..9226)
                     /locus_tag="PKM27_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNR08470.1"
                     /translation="MKIIPKGEFIFLEGPKPRTFELIFAWKVWKEFIKGFRALHFVGP
                     AITVFGGARFKREDEYYKTGLEIGKRIALSGFATVTGGGPGLMEAVNKGAFENGGKSI
                     GINILLPFEQYANPYLHKSVTIDYFFVRKTLLLKYSYAFIVLPGGFGTMDELYETITL
                     MQTNVVHDFPVVLIGKAYWKKMFEFIDEMVAYKTISPQDPGLIKVTDSLDEAMEYIKA
                     HIDTHFRVAQRHKPIWWLFERS"
     gene            complement(9259..10845)
                     /locus_tag="PKM27_14220"
     CDS             complement(9259..10845)
                     /locus_tag="PKM27_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR08471.1"
                     /translation="MKCKIFFSFFILTITQGFSQKIYNPAQIEALKAEVKKSIDGQAK
                     MAQVMIDKVFSFAELGFQETETSEYLTGILEKNGFKIERGISGVPTAWWASWGKGRPV
                     IAIGSDLDCIPKASQKPGVAYHDPIVEGAPGHGEGHNSGIPLNILSVLAVKEIMQRDQ
                     IPGTLILWPGVAEELVGTKAYFTRDGYFDSVDLCIFTHVSSNLGVSYGQAQGTGLISV
                     EYEFEGEAAHAGFAPWRGRSAADAVELMSIGWQYQREHLDPLQRSHSVIKNGGDQPNV
                     VPSKASIWYYLRHITYPQIMNLFEKANRIADGAALMTGTKVNRKILGSAWPRHFNQPM
                     AEAAYENIRQVGLPAWSDADQMLAKALQQEVGAVNKEGLASKLDTLEAPKSSPVSGGS
                     DDIGDISWKLPTITLTYPSNIPGLQGHHWSNAVTMATPIAHKGVIAGAKVEAMTLIDL
                     FTKPEITRRAWEYFRKEQGMEIKYTPMVGPQDKPAVYLNKEIMDEFRPKLRPYYYDET
                     RYSTYLEQLGIKYPTLKAKS"
     gene            10928..11776
                     /locus_tag="PKM27_14225"
     CDS             10928..11776
                     /locus_tag="PKM27_14225"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR08472.1"
                     /translation="MHILDAHQHFWKYEPAYYDWIDDKMDILRQDYFPDDLHATYAAN
                     QVDGCVSVQAISTENETDILLQHAGQHGFIKGVVGWVDLCDPGAGERLKHYSSQSKFK
                     GVRHVLQAEPVDFMERADFLAGLSRLAPLDLTYDILIYPRHLFTAWEMVKKFPDQKFI
                     IDHLAKPMIKSKQFEHWKDLMRNFADLPNVYCKVSGLVTEADWKNWRYPDFLPFLQAV
                     SEIFGPGRLVFGSDWPVCLLAASFKNVLDIVKTFTETWSVSEKEMVFYKNAVQFYNLD
                     RSWISE"
     gene            11761..12543
                     /locus_tag="PKM27_14230"
     CDS             11761..12543
                     /locus_tag="PKM27_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR08473.1"
                     /translation="MDLGIKDKVFIITGGAKGIGRAIALTMVAEGARPVILDKDDQAG
                     AQLVREIKEAGGHSIFLKVDLTEYDQCLDAVNFAVNHYQRLDGLLNNAGINDNVGLEK
                     GNPEAFEKSLQLNLMHYYNMAHLCLPHLKKTKGVILNTSSKTAVTGQGGTSGYVCAKA
                     GILGLTRDWAAELIPYGIRVNAMVPAEVWTPLYEWWVGTFPDPQAKKELIASKIPFGK
                     RFTTPQEIADMAVFLVSARASHITGEHVYVDGGYTHLDRALT"
     gene            12586..12915
                     /locus_tag="PKM27_14235"
     CDS             12586..12915
                     /locus_tag="PKM27_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HNR08474.1"
                     /translation="MPRFCFALDLKDDPKLIEEYERYHEAVWPDILQSLKKSGIVDME
                     IYRVLNRLFMIIEGNEEFSFEKKMEMDRANPKVQEWEELMWNYQQGLPGLAAGEKWMP
                     AKLVFKL"
     gene            12926..13699
                     /locus_tag="PKM27_14240"
     CDS             12926..13699
                     /locus_tag="PKM27_14240"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNR08475.1"
                     /translation="MFDLSRKTAVITGAASGIGKAIATVLAARGAEVIIFDLNEEAAA
                     QTVAEITESGGNCRAVTADVSDLEQIKSRIAAIETESQVDLLINNAGIGHVGTAENTG
                     PDYFDRLMTINVKGYYHMIHAVLPYMIKRAAGVILNIASVAGTVGISERFAYSVTKGA
                     VLAMTRSVAKDFIRHGIRCNSISPGRVHTPFVDGYLAKNYPGREAEVFAQLEKTQPLG
                     RMGKPVEIAYLALYLCCEESAFVTGTDFPIDGGFITLNS"
     gene            13734..14594
                     /locus_tag="PKM27_14245"
     CDS             13734..14594
                     /locus_tag="PKM27_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNR08476.1"
                     /translation="MKLIRFGRPELEKPGLILADGRWIDVSDVIPDYDESFFASGQVR
                     DLQEYLDTADGFRPVDPDTRLAPPLARPSKLICVGLNYAGHARETGADIPKEPVLFFK
                     STTAICGPNDDLLIPPGSVKTDYEVELAIVIGQKASYVPLDKADDFIFGYCLHNDYSE
                     REFQLERGGQWVKGKSCDTFAPLGPFILTKEEVPDPQNLRLWTKLNGELVQDSSTADM
                     VFGVHYLVHYISQFMTLLPGDVITTGTPFGVGMGRKPQRFINQGDVIEIGIDLLGSSK
                     QKARNVVSLR"
     gene            14635..15909
                     /locus_tag="PKM27_14250"
     CDS             14635..15909
                     /locus_tag="PKM27_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNR08477.1"
                     /translation="MELLIILLLILLNGLFSMSEIALVSSRKSRLETAVKKGDQNAKI
                     ALATLQSPTRFLSTVQIGITLIGILMGIYSGETITKDLELWVERFEDLRPYSHSIAVT
                     IVVIIMTFFSLVLGELVPKRIGMAYPETISKITARPMKTLSMIAAPFVWLLSHTGDLL
                     IKLFRIKNTPEGKVTEEEIKQIIQEGTEGGEVQEIEQDIVERVFHLGDRKISSLMTHR
                     NEIVFLDINDPADLIRNTVSNELHSVYPVMDGDKDNIQGVVFLKDLFTHIDDDQFRLA
                     EYLKPPKFLIENTSAYKALQLFKETKLHFGIVTDEYGQVQGMVTMNDLLEALVGGADE
                     FYDEEFSFEEREDGSWLIDGHYPIADFLHYFGLDELASKFEFNTLGGLVLHELKHIPR
                     AGERTMWKDFILEVIDMDGARIDKILVKKQAD"
     gene            16040..16633
                     /locus_tag="PKM27_14255"
     CDS             16040..16633
                     /locus_tag="PKM27_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNR08478.1"
                     /translation="MVPQFLIDLREFISQPWPWWLGGMMIAFIMFLLLWFGKNFGISD
                     NFRIMCAADGADQSTDFFKFNWKDQEWNLLVALGAVFGGYLASHYFTGPEGAVAHVSP
                     ATVESLRNLGLSYPNDQVPLVPEWYSWEAIFSLRGILCIVGGGFLVGFGARYAGGCTS
                     GHAISGLSALELPSLVSVIGFFLGGLVMTYLILPLLI"
     gene            16680..17087
                     /locus_tag="PKM27_14260"
     CDS             16680..17087
                     /locus_tag="PKM27_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNR08479.1"
                     /translation="MSRFIFIGILLGFTLYKTEAVSFFRIFEMFHFQSFHMFGIIGTA
                     VALGVGVVYLIRHFHLKSVEGQEIVIPPKAKTYPRYILGGFIFGLGWALAGICPAPMF
                     ILMGSGYTVLVLFLAAAMTGTYIYGKFRSKLPH"
     gene            complement(17158..17847)
                     /gene="trmD"
                     /locus_tag="PKM27_14265"
     CDS             complement(17158..17847)
                     /gene="trmD"
                     /locus_tag="PKM27_14265"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNR08480.1"
                     /translation="MRLDIIAALPRYFDSVFDNAIYRRATESGLFRVEVHDLRDFGKG
                     TYRQIDDYAYGGSAGMVLMAEPLSACIENLKSQRSYDDIIYLSPDGEVFNQHLANRLS
                     LCQNLLLICGHYKGIDQRIRDLYVTREISIGDYVLSGGEIAAAVLTDAVLRLIPGVMG
                     DESSALTDSHQDGLLSPPVYTRPAEFKGLKVPEVLLGGNDKAIREWLHEQALERTRDR
                     RPDLWEQWGDG"
     gene            complement(17852..18526)
                     /locus_tag="PKM27_14270"
     CDS             complement(17852..18526)
                     /locus_tag="PKM27_14270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNR08481.1"
                     /translation="MKNLLTLFFSLAFLQISFSQTMTISKDSVITDSLSYSLGTLIGS
                     NLKSQGFNELHIEDFTKGVLHAILEIGTLDLNQCSSIVDKYLAEKRELTYGPIKEAGE
                     KYLAENLKKPNIHVSKSGLQYEILTEGQGRKPGMSDTVTVHYEGKLIDGTVFDSSIKR
                     GEPIQLITNQVIAGWTEALQLMSVGSKYRLYIPYYLGYGENGAGQAIPPYATLIFDVE
                     LIKIGS"
     gene            complement(18532..19908)
                     /locus_tag="PKM27_14275"
     CDS             complement(18532..19908)
                     /locus_tag="PKM27_14275"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNR08482.1"
                     /translation="MMPEIRAYIDEHKSRFLDELIGLLRIPSISADPKYKPDVDRTAE
                     EVAAHLSKAGVDKVQVYSTPGHPVVYGEHILDPSLPTVLVYGHYDVQPPDPLELWTTS
                     PFEPEIREGNIYARGAADDKGQFFMHIKAFEAMKALGALTCNIKIIIEGEEEIGSNHL
                     ELFLKDHRNMLACDVVLISDTALIANDVPSLTTGLRGMSYVEVEVTGPNRDLHSGVYG
                     GGVANPANVLARMISSLHDDQGRVTIPGFYDKVKPVSTEERKEMNEAPFDLEEYCRDL
                     GIEEVAGESGYTTLERTSIRPTLDVNGIWGGYIGAGAKTVLPSKAFAKISMRLVPDQD
                     PEEITRLFTDYFISLAPSSVKVRVFPHHFGLPAVTPTDSLEYKAAELAMTDSFGKKPI
                     PQRGGGSIPIVSLFEQILGAKTVMMGFGLDSDNIHSPNERYGLFNFYKGIETIPLYYH
                     HYAQLKKS"
BASE COUNT         4933 a         5307 c         5093 g         5051 t
ORIGIN      
        1 tcaggaaaat gccaagacta accgcaactg gtctgccgat tatatcaata cctggcagcg
       61 ggtttatgaa ccaaaagttt acgaaggcgg ttatccgaga atagtcattg ttcccaatcc
      121 tgatggaact gtgactgcca cttttgaccg tgcagcagct tccaaatgga ttgcggacaa
      181 tcagaacctg ctcactcaat ggcaccggga aaacaataaa cttgcagaca aaggcaatcc
      241 gatcaacaat tcccttgacc gtttcgaacc cggaacagct cgatacgacc aggccctgga
      301 cgaaatccgg aacacctata ataatgaagg aggcacccgg tttttcgacc agtccgccct
      361 ttatcatgtc catggggaat ataaattcac accgaaattt gtgaacacct ggacggtggg
      421 agccaatgcg aggttgtatg cccctgaaag ccgcggcacc atcttctacg attccgccgg
      481 actcaagatc acgaattttg aatacggtgt ttataccggc atggaaaaaa agaccaacga
      541 tgaccggtgg actttttccg gtaccattcg cctggataaa aatgaaaatt tcgatttcct
      601 gatgagcccc gccgcttcgg tggtgttcaa accccgggca aacaatttcc tgcggctctc
      661 ctttgccagc gcgatccgga atcccaccct ctcggatcag tacctgcacc tgaatgtggg
      721 accggccatc cttgccggca atatcgacgg ggtccaaaac ctgatcacgg tttcttcctt
      781 cctggattat gtcaacaccc tgaaaacgga ccaattggtc tattttaata tagatcccat
      841 ccagcctgag aaagtgaaaa cctttgaatt gggctatcgc accaccctct tcaacaagtt
      901 atacgccgac ctgggatatt attacagcat ttacaatgac tttctcggtt acaatatcgg
      961 catcgatgcc acctttgacc cggtatcggg ttttccccaa cgcctgcaag cctatcgtta
     1021 cgcggccaac agcatcaacc aggtgaccac ccagggcgct gcggccggat taaattatta
     1081 tttcggcact tattaccaga tccagggaaa ttattcctgg aaccggctca ataaggaatt
     1141 tgaagacgat cccatcatcc cggccttcaa taccccggag cataaataca atatcgggct
     1201 cagcggcagg gatatccccc tgaaggtggg agggctgcga ctgaatgact ttggatttaa
     1261 catcaattac aagtggatcg attccttcca atttgaaggc tctccccagt ttacgggttt
     1321 tatcccttcc tacaacctgc tggacggaca aatgaactgg agaataagac cctggaatac
     1381 tacggtcaaa atcggggcgt caaatctcct ggacaataaa cagtttcagg cctacggagg
     1441 cccgagaata ggaaggctgg cctatctcac gcttctgtac gaatggaaaa aaaattaacc
     1501 gggaatttct caaattctca atattttacc tttataatct aaaatccttt atgaaacaag
     1561 caagatttgt gagcctcctc ctcatggctg tatggacctt tacggcatac ggtcagggaa
     1621 taagatatct tcacccggtc tttgactccg tctcggtctc gaataccttt tacgggatga
     1681 atgtgacgat actttctgta cctaccaccg gtaaggggac gcgggttccc ttacccgtca
     1741 gcatttactc cccgaccgga gatacggtca aaaaccggcc attggtcatt tacatccata
     1801 ccggaaattt tcttcccttt ccccaaaacc agggaaccgc agggagaacg gtcaatggag
     1861 gagccctggc cgccctcggc atgggctttc ccgtcgattc tccgctcgtg gaaatctgta
     1921 cccggcttgc gaaaatgggt tacgtcgtgg ctgccatcga ttaccggacc ggctggaatc
     1981 cggtagccag cacccagacg gaaagggtca ataccctgat taacgctgcc taccggggcg
     2041 tacaggattt tagaaccgcc gtccgttttt tcaaaaagac ctttaaagaa gccggtaatc
     2101 cgtatgggat tgacacttca aaaatcgttg cctggggcca gggctccgga ggatatgtga
     2161 gtttggcttc cagtgttctg gataattacc tggatatccc cctggcttct aagggtaaat
     2221 tcattggtcc tgatattacc ggggatggat ttggagatcc ctatgtattg cctcccgttc
     2281 atggagatat ctatggaact tccttgggga tcaatccgac aaacggggat accttgtccc
     2341 tccccaacca tgtagggtat agcagcgatt ttcaattgtc ggtaaacatg ggcggcgcct
     2401 tacccgatac cctgtggctg gatgccaccg acttgcctct gatctctttc caggaaccca
     2461 gggatccttt cgccccctat aaagaaggat tggttctcgt gccgagggga accatggccc
     2521 ccctgcccgt agtggaagtc cagggatcct acctggctgt agagaaagca cgccgcctgg
     2581 gcctccagaa aaaaatgatt gatcttaaac tcaacgatgt atacacccag gcagccaatg
     2641 tcaaaaacga tgggatagaa ggcctttatc cgatcagtgg caaccccctt aatcctttgg
     2701 attccgatcc ctggaactgg tgggatacgg cttttatggc ttcagtagaa cgtcaggctg
     2761 gtacaggtaa caataccaat ggcctgctga ccaacccgga catgtcggcc gcaaaagcca
     2821 gacggtatat cgataccatc atcggctact acgccccccg tgcctttgcc gttctcaacc
     2881 tgggtacctt tgtcaatacc ccggacctcc tcaaagacac cgatgtcggt ttcaaactga
     2941 tgcccaaccc gatggtcaac cagaccttgc ttcaaaccaa tcccgatttc ccgatgaagt
     3001 cggtcacctt gtacgatctc aacgggaggg tggtccgcaa actctccggt ttaaatacca
     3061 actcgatcac cgtgcatcga aacgagttgt cccagggagc ttatatcctt caggtcggat
     3121 tcaaaaaagg ggtgatctcc aagactctgt tgattcaata ataaagacgg atttccggtt
     3181 ttgccaatac cagcgggccg ggataaatga aaagcccggt ggcgacaccg ggcttttgtt
     3241 ttttcagggg aagggatact ttttctattc aataaatttg acctttagaa actcctgttt
     3301 gctaaggtga gcttacccta cttgtttaag cgcatccagg aacaattgca tctcctcgga
     3361 ggtccctaca ctcacccggc accagtgttt gtcattaaag taccagcttc tcaggccgat
     3421 cccctggttc tgcataacct gaacaaagga tttgccttcc ttttggatgg ggaaaatgag
     3481 aaagttggta taactgggca ggggctcata tcccagggca atcaaacctt taacagccag
     3541 ttcgcgggct tcccggtttt tcatcacgca gtattgtttg aactcctcat cctgcagggt
     3601 agccagggcg ccgtgcatcg aaggggcatt catggtgaaa tcccaggtcc ggtatttttc
     3661 gagttccttt ataaagccgg tggtggccag ggcataggct acccggagcc cggcaaagcc
     3721 gtggatcttt gaaagggtct tgccgatcat gacctggtcc cccgctctca gccggctgac
     3781 catgctgtgg cgggccgcat cggggatata gtcgttataa gcctcatcga cgaaaaccag
     3841 tgttttctta ctaacccggc tgcagaacgc gctgagctcc tccggatcca cgatcgtact
     3901 gcagggattg cccggattgc agatatagac caacttggtt ttcgcggtaa tggctttttc
     3961 catcgccccc aggtcgtgtt caaatttttt attgagcggg accttgatcc actgcccccc
     4021 gatggcggaa gcggtcgtta gcatcagatc gaaggtggga tgggcggaga ccaggtcgcc
     4081 atgttccagg ccggtgaccg cacccaccag gttgaggagt tccgacaagc ccgcgcataa
     4141 aaagacctgt tccggacgga ctccctcata agcggcaatt ttggatttga gttcgatcgc
     4201 gaccgtccgg gaatacaaat tcgcgtcctt aaaaccgtgg ataatggcca ttttagcccg
     4261 gtccgatggt ccataaggat tttcattgga ggtgagcctg gctttcaaag cgaccggggc
     4321 ttcttccagc atccaccttc cggtatgagg atgcacccgg ttgaggtatt gtttggtttc
     4381 cgcctgaagg cttagtggga aggcgctgcc caaagccagt ccgagtccgg ctttcagcca
     4441 gtcccggcga cccagtgtgg ttgacttcat gattggatga gatttggtac caaattaagg
     4501 aaaactgtgg aatagtagtc aatagtcaat agtcaatagt caatagtcag gagtcgggag
     4561 tcaatggtca atggtcagcg gtcagcttca gcattcagcc atcagcagtc agtgatcagc
     4621 aatcagcagt cagctttcag tacaggtctt cagtaaaaat tataagtcat gcacccaatg
     4681 tttaagcaaa tcggactatc tttcagaaaa taaagtttga tgaaaaacct gttaatcata
     4741 attcttatcc tttcaagtaa cctggccatg gcacaaggat cctggactac attgtttgac
     4801 ggcaaatcgc tggagggctg gcacaacttt aagaaaacga cggtctccgg atggatgatc
     4861 gaagacgggg tgctcaccac cgatgggaaa ggcggcgacc tggtgtcgga taaggagttt
     4921 ggggatttta tcctggagtt tgactttaaa gtcagtccga aaggcaacag cggcattgtt
     4981 tacaaagtcg ttgaagggga aggggaagcc tacttcgcca cttatgcttc cgggcccgaa
     5041 tatcagctca tcgacgatgt caactaccct tccaagctga atgaaaccca gaaaaccggc
     5101 gcaaattatg acctgaaccc accgaaatcc atgccctcca ggcccgccgg ggaatggaac
     5161 agcggcagca tcaccgtcaa gaaaaacaag atcacccata aagtcaacgg caagaccgtg
     5221 gtaaaatata cctatgggga tgaggcctgg aaagcggcgg tggctaaaag caagtttgca
     5281 aaatggccct atgccaatcc tcaccacagg ggcaagatcg ccttacaggg acataacgac
     5341 cgcgtttatt tcaggaatat ccggatcaaa gaactataat tttttttcac aaacccatct
     5401 tccatattct catgcaaaaa gtagccatgc tgggttcggg cttcatcggt cgtttttacg
     5461 ccaaagccat ccacaatcag cggagccggg atagagtcgt tgccatttac gcccgccggg
     5521 aagaaagcgc gaaaaaattt gcggacgact acggttgccc catctggtcc accgatatgg
     5581 aaaccgtcat tgcccatccg gaagtaacca tggtctgcat cgctctgccc aataacctcc
     5641 acgaaagcgc cgtgcacctc tgcgccaaac accgtaaaag cgtgatctgc accaaacccc
     5701 tgggccgcaa cagcgaagaa gcttaccgga tgctcaaagc cgtcgaggag gccggtatct
     5761 tcgccggtta tctggaagac ctgtgttata cgcctaaaaa tctcaaagcg gtggaaatgg
     5821 cccgcaacgg gtccctgggc cgcatcctct gggccaagtc cagggaaacc catccggggc
     5881 cgcacagcga ctggttttgg gacaaggaac aagccggcgg cggatgcatg ctggacctgg
     5941 gctgtcactg tgtggaaatc ggacgcaact acatcggcaa ggacatcaaa cccatccggg
     6001 ccttttgctg ggccgccacc caggtgaaac ccatagacgc cgaagatcac ggcatcgccc
     6061 tcatcgaata tgaaaacggg gccatcggcc agtttgaaac ctcctggtca ttccggggag
     6121 gcatggacct ccgcgacgaa gtgatgggca cggaaggcac catctggctc aacaatttcc
     6181 tgcgtaccgg ctttgaagcc ttcaccacgg gcaagggagg ggattatatt gctgaaaaat
     6241 cggaaacgaa ttcaggctgg ttgttcccgg tgggcgatga ggtcaatgaa ctggggtaca
     6301 accacatgtt taccgacatg ttttctgcgg cggaggaagg ccgcgagccg gcggaaacct
     6361 tttacgacgg gtatattgta aacgcgattc ttgatgcctg ctaccggtcc gcggaaagcg
     6421 gtaaatggga acccgtcgaa ctcaaagaat ggcggcgccg caccggcctt tcaaaattca
     6481 gcggttacca ggaatacgat gccgatcatt attttataaa agaagaagtc acccatgacg
     6541 gccggcacaa actgatcctg aaagataaga agtcggggaa gtttgttgaa aaagacatca
     6601 aggggtagct ggaagctggg agctggaagt tggggcggtc gcccaaaccg catcgcagtt
     6661 gagccaattg aagaatggga ccgacttact ttaaattcgc aggaataccc aatcacaaag
     6721 catccccgag cttctaactc ctagcttcta gctcctggaa gctgtagaag atctactatg
     6781 acctctaacc tctgacctct aacctctgac ttctgacttc tgacctatgt ccttgnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacctc taacctctga cctctaacct
     6961 ctgacttctg acttctgacc tatgtccttg catttcatat caaaactttt attatattag
     7021 atttcgaaat atttatttta aaaccaaacc ttaaatgtta tggattctat tcttcccatt
     7081 ctgatcaatg caatttttgg tggtggaggt ggctggctgg cttccatgct caagaaaaac
     7141 gggctgggcc ttgtaggtaa tttgttatcc ggggcggttg gaggcaatgt gctccccctg
     7201 atcacctcgg cgcttggcct ctttgccaac agcggtgacg gcggcagcct caaccttaca
     7261 tccatcctca ccgctttggt aggtggaggt gcgggttccc tgctcggcgg cctgtttaaa
     7321 aaagctcccg ctgcctaggc aataatcagt agtcagtagt cagtagtcag tagtcattgg
     7381 ggctcgcgtt gccgaccact gaacgctgac tactgattac ttcttttaat cttttcataa
     7441 tttattttaa ccctgcttaa cgcatgattt gcgtgtttat tccaaacatg ccaatcatga
     7501 aaacatttta caccatcgct tctgccctgt ttatcgcggt caacgcattc gcgcaacagg
     7561 gccttgaact gagtatcggt ccatctttca ccaacgtttc agtccggggt ttacagtctt
     7621 ccctgaggcc cgaccggacg ctgcacacgg gactgcaagt gcaggcttta tgggaccagg
     7681 acctgggcag cggtttttcc atggctaccg gactcggata caaggaaaag ggatttcatg
     7741 tccgcgaggg gatcgatatg gatgtattta atcttccggt cagtctcgga gtggaagcca
     7801 tcactaccct gaaatacctg gaacttcctt tgcaatggag atatcatacc cagggagacg
     7861 gcccgcttgg attctttgta tttgccggcc ccgccctctc ctatgccctg gaaggggagg
     7921 tccgcacccg ggccaacctg atctttgatg tcaatctgac aaaaacaaaa ctcaacctgg
     7981 accaggatat atacaaccgc atggaatgga gcggcctggc cggtgtcggg gggaccctaa
     8041 ggcttaaaag cggcgccctg actgtccagg cagggtatca gcatggcttc agccgtgtgg
     8101 tggataatac cctcatcgac ctccgcctca ggaattacgg atttggcatg aatattggat
     8161 accggataaa cttgtaagga cattttttaa tcccaaaaca gagtgtggaa acccagaccc
     8221 ggggtttccg cacttttttt tgccccttat cccttcctcc ttttctccct tcctcctttc
     8281 ctgcccttgt ttgcaaaatc cttctatttt tgtggcgctt taatacaaga atcatgaatc
     8341 agacaaaaag tgaatccagc cggctcatgt gggtctatac cctgatatcc ctggttatta
     8401 tgttgttttt gctggccgtc aaaccggaat ggttttgggt tgccctgccc ttcttcggta
     8461 cctacctggt caaggctttt ggcgtgattt aaattaaaaa ccttcaggac ctttcgaaaa
     8521 gccaccagat gggtttatgc ctctgggcca cccggaaatg ggtatcgata tgcgccttga
     8581 tatactccat cgcttcatcc agtgaatccg tcacctttat caggccggga tcttggggtg
     8641 aaatggtttt gtaagccacc atttcatcga taaactcgaa catttttttc cagtaagcct
     8701 tgccgatcag cacgaccggg aaatcgtgca cgacgtttgt ttgcatgaga gtgatcgttt
     8761 catacaactc gtccatggtc ccgaaacctc cgggcaatac gataaaggcg taagagtatt
     8821 tcaacaggag ggtcttgcga acgaaaaaat agtcgatggt gacggatttg tgcaggtaag
     8881 gattggcgta ttgttcaaag ggcagcagaa tattgatccc aatggatttg cctccgttct
     8941 cgaaagctcc tttgttgacg gcctccatca ggccggggcc gccccccgta acggtggcaa
     9001 aaccggacag ggcaatgcgt ttgccgattt ccaagccggt tttgtaatat tcatcttccc
     9061 ttttaaaacg ggctcccccg aagaccgtga tggcggggcc tacaaaatgg agggcgcgaa
     9121 agcctttgat aaattctttc caaaccttcc aagcgaaaat aagctcgaaa gttctgggct
     9181 tggggccttc aaggaatata aattcccctt ttggtatgat tttcaaggaa taaccggtta
     9241 atgagtttga agtagggatc aggatttggc tttgagggtg ggatatttaa ttcccagttg
     9301 ttccaggtat gtgctgtatc tggtttcatc gtaataataa ggccgcagtt taggacggaa
     9361 ttcatccatg atctccttat taagataaac agcgggtttg tcctgaggac ccaccatcgg
     9421 ggtgtacttg atctccatac cctgttcttt tctaaaatat tcccaggccc ggcgggtgat
     9481 ttcgggtttg gtaaaaaggt cgatcagggt catggcctcc actttcgccc cggcgatcac
     9541 ccctttatgg gcgatggggg tagccatggt gacggcattg gaccagtgat ggccctggag
     9601 gccaggtata ttggacggat aggtcagggt gatggtaggc agtttccagg atatatcccc
     9661 gatatcatcg gatccaccgc tcaccgggga cgatttaggg gcttccaggg tatccagttt
     9721 actggccaat ccttctttgt tgacggcacc cacttcctgc tgcaaagctt tggccagcat
     9781 ttgatcggca tcggaccagg caggcaggcc cacctgcctg atgttttcat aagcggcttc
     9841 cgccatgggt tggttaaaat gcctcggcca ggcggagccc agaattttac ggtttacttt
     9901 agtcccggtc attagggctg caccgtccgc gatgcggttt gctttttcaa agaggttcat
     9961 gatctgagga taagtgatat gcctcaggta ataccagatc gatgctttgg atggcaccac
    10021 attcggctgg tcccctccgt ttttaatcac ggaatgtgaa cgctgtaacg ggtccaggtg
    10081 ctcgcgctgg tattgccatc cgatgctcat cagttccacg gcatccgccg cgctcctgcc
    10141 cctccaggga gcaaagccgg catgggcggc ctccccttca aattcatatt cgaccgagat
    10201 cagaccggta ccctgcgcct gtccgtaaga gaccccgagg ttgctgctga catgggtgaa
    10261 tatgcagagg tccacggagt cgaaatatcc atcgcgagtg aaataggcct tggtccctac
    10321 aagttcttca gccacgccgg gccagagaat gagggtgccg gggatctggt cgcgctgcat
    10381 gatttctttc acggcaagca cgctgaggat gttgaggggt attccgctgt tatgcccttc
    10441 tccatgcccc ggagcccctt ccacgatcgg gtcgtgatag gccacccccg gtttttgcga
    10501 agctttgggg atacaatcca gatcggaacc aatggcgatc accggccttc cctttcccca
    10561 gctggcccac caggccgtgg gcactcccga aatcccacgt tcgatcttaa atccgttttt
    10621 ctccaggatg ccggtcaggt attcggaggt ttccgtttcc tggaaaccga gttccgcaaa
    10681 actgaacact ttatcgatca tgacctgggc catttttgcc tggccgtcaa tcgacttttt
    10741 gacctctgcc tttagcgctt cgatttgggc aggattgtat attttttgtg aaaatccttg
    10801 cgtgatggtt aaaataaaaa aactaaaaaa aatcttgcat ttcattggat caggaatatt
    10861 gaataaacaa acttaaataa aaaaatcaat tcacccggat tgcttacatt agcccccgct
    10921 gtaccatatg catatccttg acgcgcacca gcatttctgg aaatacgagc ctgcttatta
    10981 cgattggatc gatgataaaa tggacatact caggcaggat tatttcccgg acgatttgca
    11041 tgccacctat gccgccaacc aggtggacgg atgtgtatcg gtccaggcga tctccacgga
    11101 aaacgaaacg gatatcctgc tgcagcatgc cggacagcat ggcttcatca aaggagtcgt
    11161 aggttgggtg gatctttgcg atcccggcgc gggggaaaga ctcaaacatt attcatccca
    11221 atccaaattc aagggggtac gtcatgtcct ccaggccgaa ccggttgatt ttatggaaag
    11281 ggcggatttc ctggcagggc tgagccggct ggcaccgctg gatttgactt acgacatcct
    11341 gatctacccg aggcatttat tcaccgcctg ggaaatggta aaaaaatttc ccgatcagaa
    11401 attcatcatc gaccacctgg ccaaaccgat gattaaatca aaacaattcg aacattggaa
    11461 agacctgatg cggaatttcg cggaccttcc caatgtctat tgcaaagttt cgggattggt
    11521 gaccgaagcg gactggaaaa actggagata tcccgatttt cttcctttcc tccaggccgt
    11581 atcggaaatc ttcgggcccg gacgcctggt ctttggttcg gactggccgg tctgcctgct
    11641 cgcagccagt ttcaaaaatg tattggatat cgtcaaaacg tttacggaaa cctggtcggt
    11701 tagcgaaaaa gaaatggtat tttataaaaa tgcggttcaa ttctataact tagaccgatc
    11761 atggatctcg gaataaaaga caaagtcttt atcatcaccg gcggggccaa gggcatcggc
    11821 agagccattg cccttaccat ggttgccgaa ggcgcccgtc cggtcatcct ggacaaggac
    11881 gaccaggcgg gggcgcaact cgtccgtgaa atcaaggaag ccggcggaca cagcatcttc
    11941 ctcaaggtgg acctcacgga atacgaccaa tgcctggacg cggtaaactt tgcagtgaat
    12001 cattaccaaa ggctggatgg tttactgaac aatgcaggca tcaacgacaa tgtgggcctc
    12061 gagaaaggca atcccgaagc ttttgaaaaa agccttcagc tcaacctgat gcattattac
    12121 aacatggccc acctttgcct cccccacctg aaaaaaacca aaggagtgat cctcaatacc
    12181 agctccaaaa cggcggtcac cggccagggc ggtacctcag gctatgtgtg cgccaaagcc
    12241 ggcatcctgg ggctcacccg ggactgggcc gccgaactga tcccatacgg catccgggtc
    12301 aatgccatgg tccctgcgga agtctggacc ccgctctatg aatggtgggt gggcaccttc
    12361 ccggatcccc aggccaaaaa agaattgatc gcctccaaga tcccttttgg aaaaaggttt
    12421 accacgcccc aggaaatcgc ggatatggcg gtcttcctcg tttcagcaag ggcttcacac
    12481 atcaccgggg aacacgtgta cgtcgatgga ggatataccc atctggaccg cgccctcacc
    12541 tgatggatcc tttgaattta attttattgc ctagaaatcc cgttcatgcc ccgattttgt
    12601 tttgcccttg acctcaaaga tgaccctaaa ctcatcgaag aatacgaacg ataccatgaa
    12661 gcggtatggc cggacattct ccaaagcctc aaaaaatccg gcatcgtgga catggagata
    12721 taccgggtat tgaaccgcct ttttatgatc atcgaaggca acgaagagtt cagttttgag
    12781 aaaaaaatgg aaatggacag ggcaaatccc aaggtacagg aatgggagga actgatgtgg
    12841 aattaccagc agggcctgcc cggcctggcg gccggtgaaa aatggatgcc ggccaaactt
    12901 gtttttaaat tataattccg atgagatgtt tgacctaagc cgtaaaaccg ctgtgatcac
    12961 gggggcagcc agtgggatag gcaaagcgat tgccaccgtc ctcgccgccc gcggcgctga
    13021 ggtcatcatc tttgacctta acgaagaggc cgcagcgcag accgtcgcgg aaatcacgga
    13081 atccggcgga aattgcaggg cggtgaccgc ggacgtcagt gacctggagc agatcaaatc
    13141 gcggattgca gccatagaaa cggaatcgca agtggacctt ctgatcaaca atgccggcat
    13201 cgggcacgtc gggaccgcgg aaaatacggg cccggactat tttgaccggt tgatgaccat
    13261 caacgtaaaa ggctattacc atatgatcca cgccgtatta ccttatatga tcaaaagggc
    13321 agcaggggtc atcctcaaca ttgcctccgt agcgggcacc gtgggcattt cggaacggtt
    13381 tgcttattcg gtcaccaagg gagccgtgct ggccatgacg cgctcggtcg ccaaggattt
    13441 tatccgtcac ggcatccgtt gcaacagcat cagcccgggc agggtgcata cgccctttgt
    13501 ggatggttac ctggccaaaa actatccggg ccgggaagcc gaggtttttg cccaacttga
    13561 aaaaacacaa ccccttggcc gtatgggaaa accggtggaa atcgcctacc tcgcccttta
    13621 cctgtgctgc gaggaatctg cctttgtcac cgggaccgat tttccgattg acggaggatt
    13681 catcaccctc aattcctgat catcttgctc atccaaataa atacgacaga ataatgaaac
    13741 tcatccgttt tggccggcct gaattggaaa agcccggcct gattcttgca gacggccgct
    13801 ggattgatgt cagcgatgtc attccggatt atgatgaatc tttttttgct tccggacagg
    13861 tccgtgattt gcaggaatac ctggataccg cggatggctt tcgaccggtc gaccccgata
    13921 cccgcctggc cccccctctg gcaaggccct ccaaactgat ctgtgtgggc ctgaattatg
    13981 ccggccatgc ccgcgagacc ggcgccgata tcccaaaaga accggtgctt tttttcaaat
    14041 ccacaacggc gatttgtggt ccgaacgacg acctgctgat cccacccggt tcggtcaaaa
    14101 cagattatga agtggaattg gctattgtga tcggtcaaaa agcgtcttac gtaccccttg
    14161 acaaagcgga cgattttata ttcggttatt gcctgcacaa cgactacagc gaaagggaat
    14221 tccagctgga aagaggcggt cagtgggtga aggggaagtc ctgcgacacc tttgcccccc
    14281 tggggccctt tatcctcacc aaagaggagg tgcccgatcc ccaaaatcta agactttgga
    14341 cgaaactcaa tggggagctg gtccaggatt cttccactgc cgatatggtc tttggggtac
    14401 attacctggt acactatatc agccagttta tgaccttgct tccgggggat gtcatcacca
    14461 ccgggacccc ttttggagtc ggaatgggaa ggaaaccgca acgatttata aatcaaggag
    14521 atgtgatcga aatcggcata gatctcctgg gcagttctaa acaaaaagca agaaatgtcg
    14581 tatcattgcg ctgaaatctt acagacctga gattttggcg gtttagttac gagcatggaa
    14641 ttgttgatca ttttgttgct gattttgctg aatgggctgt tttccatgtc agaaattgca
    14701 ctggtatcct ccaggaagtc gaggctggaa accgcagtaa aaaaagggga ccaaaacgct
    14761 aaaatcgcgc tggccaccct tcaatccccc acccgttttc tttccaccgt tcagatcggg
    14821 atcaccctga tcgggatcct catgggtatc tatagcggcg aaaccatcac caaggacctg
    14881 gaactgtggg tggaaagatt cgaagacctg aggccctatt cccacagcat agcagtcacc
    14941 atcgtggtca tcatcatgac ttttttctcc ctggtccttg gagaactggt tcccaagcgc
    15001 atcgggatgg cgtatccgga gaccatatcc aagattacgg cgcggccgat gaaaaccctt
    15061 tccatgatcg ccgccccttt tgtctggctt ctctcgcata ccggtgacct cctgatcaaa
    15121 ttgttccgca ttaaaaatac cccggaaggc aaggttacgg aagaagaaat caaacagatc
    15181 atccaggaag gaaccgaagg cggtgaagtc caggagatcg aacaggacat tgtagaaagg
    15241 gtttttcacc tgggagaccg gaaaatatct tccctgatga cccaccgcaa cgagatcgtt
    15301 ttcctggaca tcaacgaccc ggcagacctc atccgcaata ccgtgagcaa cgaactccac
    15361 tccgtatatc cggtgatgga tggggacaag gacaatatcc aaggggtcgt tttcctcaag
    15421 gaccttttta cccatattga cgatgaccag ttccggcttg ccgaatacct caaacctccg
    15481 aaattcctga tcgaaaacac cagcgcctat aaggccctcc aattattcaa ggagactaaa
    15541 ctgcatttcg gcattgtcac ggacgaatat ggccaggtcc agggcatggt gaccatgaac
    15601 gaccttttgg aagccctggt cgggggagcc gatgaatttt acgatgagga attttctttc
    15661 gaagaacggg aagacggttc ctggctgatc gacggccact atcccatcgc ggatttcctc
    15721 cattatttcg gcctggacga actggcttcc aaatttgagt tcaataccct gggtggcctc
    15781 gtcctgcatg agctgaaaca tattccccgc gccggtgaac ggacgatgtg gaaagatttt
    15841 atcctggagg tcattgatat ggatggggca aggatcgaca agattttggt aaaaaaacag
    15901 gcagactgat ccccgctccc ccgagctgtc cctgccggcg tttcgatatt ttccaaccga
    15961 ttttttatcc tttatttttt ttatttcttc cgggccgcct tcgcaaatta tctttatccc
    16021 attctaaatt tccgccgtta tggtacctca atttctgatc gatctccgtg aattcatcag
    16081 ccagccctgg ccctggtggc tgggcgggat gatgattgcc ttcatcatgt tcttattact
    16141 ctggtttggc aaaaatttcg ggatttccga caatttcagg atcatgtgcg cggccgacgg
    16201 agccgatcaa agcactgatt ttttcaaatt caactggaaa gaccaggaat ggaatctcct
    16261 cgttgccctt ggcgcggtct tcgggggcta cctggcttcc cattatttta ccggccctga
    16321 aggcgcggtg gcccacgtct ctcccgcgac ggtcgaaagc cttcgcaacc tcggcctttc
    16381 ttacccgaat gatcaggtgc ccctggtacc cgaatggtat tcctgggagg caattttcag
    16441 tctccggggt atcctctgta ttgtcggggg aggcttcctc gttggatttg gcgcccggta
    16501 tgccgggggt tgcacttcgg gccatgccat cagcgggctt tccgccctgg aactgccctc
    16561 cctggtctcc gtcattggct tttttctggg cgggctggtg atgacttacc tgatcctgcc
    16621 tttgttgata taaaccccaa gacctctttt attcactgta aaaatttctc ggtatgcgga
    16681 tgtcacgatt tattttcatc ggaatcctcc tcgggttcac tttatataag acggaagcgg
    16741 tttctttttt ccgcatcttc gaaatgtttc attttcagtc cttccacatg tttggcatca
    16801 tcggcactgc ggtggcgctc ggtgtcggcg tggtttacct gatccgccac tttcacctca
    16861 aatcggtcga aggacaggaa atcgtgatcc cccctaaagc caaaacctat ccccgttata
    16921 ttctcggcgg ttttattttc ggcctgggct gggctcttgc cggcatttgt cccgccccga
    16981 tgtttattct catgggcagt ggatatacgg tactggtcct gtttttggca gcggctatga
    17041 ccgggactta tatttatgga aagttcagga gcaaattgcc gcactgaggt gttgaattgt
    17101 ggaattgcgg aactttcgaa ctaacaaact tgctaacttg ctaacttgca aactgtttca
    17161 tccatctccc cattgctccc atagatccgg cctgcgatcc cgcgtcctct ccagggcctg
    17221 ttcgtgcagc cattcccgga tggccttatc gttgccaccc agtagcactt cgggcacttt
    17281 taatcctttg aattctgccg ggcgggtata aaccgggggg gacaacaaac catcctggtg
    17341 cgagtcggtc agtgcggaag attcatctcc catcactccg gggatcaggc gaaggacggc
    17401 atcggtcaaa accgccgccg cgatctcccc cccggacaag acgtaatccc cgatggatat
    17461 ttcccgcgtc acataaaggt cccgtatgcg ctgatcgatc cctttgtaat ggccgcagat
    17521 cagcaggagg ttctggcaga gggacaagcg gttggcgagg tgctggttga atacctctcc
    17581 gtcggggctc agataaatga tatcgtcata ggaacgctgg cttttcagat tttcaatgca
    17641 agccgaaagc ggttcggcca tgaggaccat gccggcgctg cccccatagg catagtcgtc
    17701 gatctggcgg tacgttcctt ttccaaagtc tctgaggtcg tgaacttcaa cgcggaaaag
    17761 tccggattct gttgccctcc ggtagatggc attgtcaaaa acgctgtcga aatagcgggg
    17821 aagggctgct atgatatcca gccgcatcgg atcatgaacc tattttgatc aattccacat
    17881 cgaaaatcag ggtggcataa ggcgggatcg cctgtccggc gccgttttct ccgtaaccga
    17941 ggtaataagg aatgtacaag cggtatttgg aacccacgct catcaattgt aaagcctcgg
    18001 tccatccggc gatgacctgg ttggtgatca gttgaatggg ctctccccgt ttgatggaac
    18061 tgtcgaaaac cgtgccgtcg atcagtttgc cttcgtaatg caccgtgacc gtatcggaca
    18121 tcccgggttt tcggccctgg ccttcggtaa ggatctcgta ttgcaggcct gatttcgaaa
    18181 catggatatt tggttttttc aggttttccg caaggtattt ttcaccggct tctttgatcg
    18241 ggccataggt caattccctt ttctccgcca ggtatttgtc cacaatggat gaacactggt
    18301 tgaggtccag agtcccgatt tcgagaatgg cgtgcagcac gcctttagta aagtcttcga
    18361 tatgaagttc attgaagccc tgggacttca gattggaacc gatcagggtc cccagggaat
    18421 aagaaaggct gtcggtgatc acggaatctt tggaaatagt catggtctgg ctgaaagaga
    18481 tttgtaaaaa agcaagggaa aagaaaaggg ttaacaggtt tttcatgaat gtcaggattt
    18541 ttttaattga gcataatgat ggtaatagag cggaatggtt tcaatgcctt tataaaaatt
    18601 gaaaaggccg tatctttcat tgggtgaatg gatattgtct gaatcgaggc caaaccccat
    18661 catgaccgtt ttggcgccga ggatctgttc aaaaagcgaa acaatcggaa tgctcccccc
    18721 tccgcgctgg ggaatgggct ttttaccaaa actgtccgtc atggccagtt cggcagcttt
    18781 atactccaaa gaatccgttg gggtgaccgc aggaaggcca aaatgatgag ggaagaccct
    18841 gaccttgacg ctggatggag ccagggatat gaaataatcc gtaaacaacc gggtgatctc
    18901 ctcgggatcc tggtccggta ccagccgcat gctgatcttg gcaaaagctt tggagggcaa
    18961 tacggttttg gcccctgccc caatgtaccc accccatatt ccgttcacat ccagggtcgg
    19021 ccggatcgaa gtccgttcca gggtggtata acccgattcc cccgccacct cttcaatgcc
    19081 caggtccctg caatattctt ccaggtcaaa aggggcttca ttcatttcct ttcgctcctc
    19141 cgtggatacc ggtttaacct tgtcgtaaaa cccggggatg gtgacccggc cttggtcgtc
    19201 gtgcagggag gagatcattc ttgccaggac attggccggg ttggccacgc cgcctccata
    19261 gacccctgaa tgcaggtcgc ggtttggacc ggtgacctcc acttcaacat aactcatccc
    19321 acgcaggcct gtcgtcaggg aaggaacatc gttggctatc agagcggtgt cgctgatcag
    19381 cacgacatcg caggcaagca tgttccggtg gtctttgagg aagagttcca gatgattgga
    19441 accgatctct tcctcccctt cgatgatgat ctttatattg caggtcaagg ctcccaaagc
    19501 tttcatggct tcgaaggctt tgatgtgcat aaaaaactgt cccttatcgt cagcggctcc
    19561 ccttgcatag atgtttcctt cccggatttc aggctcaaag ggggaagtgg tccaaagttc
    19621 caggggatcc ggaggctgga catcgtaatg tccataaacc aacacggtag ggagggaagg
    19681 atccaggata tgctccccat atactacggg atgtccgggt gtggaataga cctgcacctt
    19741 gtccactccg gctttgctca gatgagcagc tacttcttcc gcagtacggt ccacatccgg
    19801 cttgtatttc gggtcggcgc tgattgaagg gatccggagg aggccaatca actcatctaa
    19861 aaaccgggat ttatgctcat cgatataggc tctgatttcc ggcatcatgg tcaaaaaaaa
    19921 tttggctgca aatatactca tctttaatca ggacgaattg aaaaaggggg aagaggggta
    19981 tgaccatccc gcacttgcaa gagcaggtct ggtttacgtc tatttcgaaa cccggaacct
    20041 ctcttatagt tggagattta aacctgctcc cccatgcacg gactgggtca ggggcgttga
    20101 taatattttc tatgaaggtc ctttttcgac ccatctatgt actccttccc aagagcaagg
    20161 aaggctgtct tgattaagga tacggccagg atccttggtt cccccgtggg agtcaggagc
    20221 gttgaagata ttttaagtta atctacccta cctcttacct cccagctcta acttctgacc
    20281 tcttacctct tacctccatc ctcttacctc tgacctctga ccacccggat ccttggttcc
    20341 ccctttaggt atactccagg aaccttggtc cccccgaggg ggacaggggc gttgaagatc
    20401 ttttatatga agattctttt ttgtattatt tctccgaccc ctgaccccta aaggggaacc
    20461 cgacctttgg tattccgata taat
//
LOCUS       DAOQXT010000088        19883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3086_length_19883_cov_5.887079, whole genome shotgun sequence.
ACCESSION   DAOQXT010000088 DAOQXT010000000
VERSION     DAOQXT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19883
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3086_length_19883_cov_5.887079"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1665)
                     /locus_tag="PKM27_14280"
     CDS             complement(118..1665)
                     /locus_tag="PKM27_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNR08483.1"
                     /translation="MESRLEIVTDPADPDQDKALRTNLARHLGLEVHELPLFRVLKRS
                     LDARRNPVYRYQVELEEAKGLPTEPTIKSRFRDVRSAPSVVIMGAGPAGYFAALECLI
                     LGLKPIVLDRGKDVKSRRRDLKAIQQDHIVNPDSNYCFGEGGAGTYSDGKLYTRSGKR
                     GDIGKILRLFVEHGADPDILIDAHPHIGSNKLPGIVEQIRNSILASGGEVHFNQRVVD
                     FVIRERRMRSVITASGDEFAGEAFILATGHSSRDIYHLLARKNLRLDAKAFALGVRVE
                     HPQQLIDQIQYRRAGREPYLPAASYSLSCTFNERGVFSFCMCPGGLMVPSATAPGEIV
                     INGMSLSRRDSRFANSGMVAAVFPSDFKNPADPFCCLTFQQEVEQAVFRAGDGSQRAP
                     AQRLTDFLNHKLSSSLPEVSYIPGIWSAPVHQVLPAFIVKSLVTGLRQFGKQMKGYIT
                     DEAVVVATESRTSSPVRIPRNSLSMHPDCEALFPCGEGAGYAGGIVSAAMDGQHCAQA
                     ALQYVLK"
     gene            complement(1667..2494)
                     /gene="kdsA"
                     /locus_tag="PKM27_14285"
     CDS             complement(1667..2494)
                     /gene="kdsA"
                     /locus_tag="PKM27_14285"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNR08484.1"
                     /translation="MNFQNLPLVNNPESPLFFLIAGPCVIESRDLIFETADHLTRITR
                     ELGIPFIFKSSYRKANRSSKDSFTGLGDVVALQYLQEVRDHFGIPVLTDIHTEPEAIL
                     AAPFVDVLQIPAFLCRQTSLLEAAADTGKTINIKKGQFMGPEAMKFAVDKVQARNNPN
                     VWLTERGTTFGYQDLVVDMRSIPVMQGLGVPVILDVTHSLQQPNQASGVTGGRPALIK
                     TMASAGIAAGADGLFIETHPNPSIAKSDGANMLALDRMESLLKHLVRIRRAILPEVS"
     gene            complement(2515..3342)
                     /locus_tag="PKM27_14290"
     CDS             complement(2515..3342)
                     /locus_tag="PKM27_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08485.1"
                     /translation="MKRYGKAEIGTWGSFILDYSPASWSVSRQSFEPPRASLQWVPGN
                     PGNFHTMASLVSKLHEVDLQEASRFLESVFLKFQTTLARQLPLELGVLGQLKPAQGSD
                     SLEFLKGPHLDALFEFAPLQLPPLTVQVPEKNPRIWIWLAIALITLTLAGLFFYFLSI
                     PSPEISATQATSRSTGPLKEVNIAPHQADTVPPEEEVAEASPLIQERIIITGTFCREG
                     NIRQMKEKIQYMQYRLYEEKVSEDCVRLGIYVYEEEDLELTLKDIRMYIEPTAWVLN"
     gene            complement(3416..4168)
                     /locus_tag="PKM27_14295"
     CDS             complement(3416..4168)
                     /locus_tag="PKM27_14295"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNR08486.1"
                     /translation="MLRDLVTSFKAYWEAWTIIVDKKLWMYLIIPGLISLFYGLTVGF
                     TAWYWSDNLKDLIYTILPTDSDSRILSKISSAIVGVGIATTGVVIYKYVIMVLLSPIL
                     NGLSRRVEEAVIGYSISYRKESDETVLKEYGRSLQFCLRNFLKEMIFTIGLLIVGLIP
                     FMGYVATPAIFLIQAMYSGYVNLDFTLGRYFSINGSIDFIRKNFGFSLGNGIAFFLII
                     MIPGVGLFFAPTLGTAAATLKAVPRIYRPSIA"
     gene            complement(4327..5712)
                     /locus_tag="PKM27_14300"
     CDS             complement(4327..5712)
                     /locus_tag="PKM27_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08487.1"
                     /translation="MEKLDSKYADRVDAIREQIQSSDHLQSYLEEETEQAFQALRDEF
                     EPEIHALYTEVGEEKPLQLIDLEQHLLDEKFEGLYLPKILGYTVLRGDVNEQVKYRRP
                     QDQFGLAVHALATHNNFEVLKNKTGQTLQIGFSLSSDIWVSNLIEHIENKRSRQYFEN
                     IRTNKIADEQERAVALKKYSRQFEGAPYHSAEFPETPEDLKSLYIPLQEFLLHRVSRF
                     RNNESLIPYLADLLKNEKLYHLSEFWSIVFIVTNFFHLEGNDRELLRRLLNENRRNSP
                     DFSEQYFNFLHSMHLAGIKFDANVDRNAAGLLDQDIQDDVSRYYQLADLIHTKGYMHE
                     DTIEAVRSFYETNEGLSVINTTVRDTILGYFKGLLENLGESEYHDYFSMSRIFNTYMK
                     IFSNEKFNQEVQGLNQQFIHKLLLKYNDKRGKDYQEIKKFVTAHFAELEYMKEKDITE
                     LFKTRRKKATT"
     gene            complement(5803..6273)
                     /gene="rpsP"
                     /locus_tag="PKM27_14305"
     CDS             complement(5803..6273)
                     /gene="rpsP"
                     /locus_tag="PKM27_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNR08488.1"
                     /translation="MAVKIRLQRHGRKKRPYYHIVVADARAPRDGKFIELIGTYNPIT
                     KPATIDINRDKALDWLGKGAQPTDTVRNILKFKGVLYKKHLMRGVKKGAMTIEEAEQK
                     YQAFIQDKDRANAQEVIKSAAEVKAFHEMVFGKAKPSRKKAQPEAPAEESAPQE"
     gene            complement(6371..8899)
                     /locus_tag="PKM27_14310"
     CDS             complement(6371..8899)
                     /locus_tag="PKM27_14310"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNR08489.1"
                     /translation="MGKILYTCMLTLLCLSMVHAQPVATITGQVRDVENNQAVDLATV
                     FVRAQNLSTETDENGRYSLQVPANIPLLLEVSRIGYKNASYPVSAIAAGTVLGIDIDL
                     VLESSSLEVIIRESNVRNRGMIRENLENLKLIPSVTGNFESILPHIALGTSSGTGGEL
                     SSQYNVRGGNYDENLVFVNDSEVYRPQLVRSGQQEGLTFPNADLIRDMSFSSGGFEAR
                     YGDKLSSVLDIHYRRPDSVKGSVSMSLLGATGHLEGSHQAGRSSYRKFTYLVGARYKT
                     NRYLLNSLQLKGEYVPDFADVQAYLTYDINKNWQLGALGNYNSSIFRFVPLSRSTATG
                     LIDFSLKLSADFEGQEIDHFRTGMGGLSLTFIPERKKNPLFIKFLGSAYQSLEKESFD
                     IISDYRLSLIETGLGSENQGKELALIGNGVQHLYARNLLYLQVRNLELKGGYEYSPGA
                     SREKTHFFQWGLRAQREEILDRLNDWERLDSALYTLPHTEQDVVIKNVLKTRNELEST
                     RFSAYFQDEYTMQRDGLGELRISGGFRMAYWTLNKEYYINPRAQVLYKPGQWKKDITL
                     RFAGGLYYQPPFYRELRTPEGLVNRDLQSQKSAHLLAGFTWDFTRGIKQPVPYRLVGE
                     VYYKKLWDQVIYDIDNVKIRYSGMNDASGYITGLDLRLNGEFVRGAESWFNLSFLRAR
                     ESIDGLQHQRREKGEAEGKNVKDVPRPTNQFLTFSALFQDYLPGNENLKVNLNLTVGT
                     GQPFGIPGNNRIFRNTYRYPAYHRVDLGFSYLIWDKSWRARRSKHLFRFTENAWISAE
                     IFNLLKVQNVASNTWFKSIYNVQYAIPNYLTSRRINIKCRIGFH"
     gene            complement(8906..9583)
                     /gene="rpe"
                     /locus_tag="PKM27_14315"
     CDS             complement(8906..9583)
                     /gene="rpe"
                     /locus_tag="PKM27_14315"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNR08490.1"
                     /translation="MKDQVLVAPSILNTDFTELGKTVDLLDQSEADWIHLDIMDGVFV
                     PNISFGIPVIQSIRKRTAKPLDVHLMIVEPDRHLEAFRDAGADLITVHYETCPNLHRT
                     LEYIRTLGAKAGVAVNPHTPVESLRDVLEILDLVLIMSVNPGYGGQKFIYQSIPKLRR
                     LKQMMAEENLNPLVEIDGGIGLQNAEPVVKAGVDVLVAGSSVFSDPDPASVIHRLRQF
                     SKSTFFV"
     gene            complement(9580..10320)
                     /locus_tag="PKM27_14320"
     CDS             complement(9580..10320)
                     /locus_tag="PKM27_14320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR08491.1"
                     /translation="MENTKAQDILFFDIETVSGKAEFSELPETMKMLWKLKANAFTRS
                     YDQPMTDAEVSEFYTQKAAIFAEFGKIICISVARFDLKEGQDLKLKVKSFFGHDEKEV
                     LLEFAEMLRKRFYRDEHRICGHNIREFDVPYLCRRMMVHQIPFPRILNLSGKKPWEST
                     HIDTMQYWKFGDVKHYTSLNLLANVLGIESPKDDIDGSQVGPVYWRENNLARIVDYCQ
                     KDAITVAQLYLYFNHKTLLSREQIEIFQ"
     gene            10619..11614
                     /locus_tag="PKM27_14325"
     CDS             10619..11614
                     /locus_tag="PKM27_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08492.1"
                     /translation="MSDYNSNNTAKKSNYLPILIGLLIASVAGNAYLFFNKQKQQEII
                     VNTTTKLTESERLKAELEKQYYEALQDLEKQRTDNKALNELIESQKVELKTQKDRIAR
                     MLGDSKNLASAKKEMANLRTQVDGYLAEINKLKEENATLATSNNMLTEEKKSLTTNLE
                     ETKKVNDELTTAKAVLISEKEKLASENTTLVKKVNIASVVKVNNIDISGYRIKSSGKE
                     VDRNKAKAIDGLRICFDVTENTITESGVEQFYVRVINPNGETQALENLGSGVLVNQAN
                     NEEIKYTSLKGVKYENKPQNTCMNWQPNIPFQEGTYTVEVYNKGYLSGTTTFQLK"
     gene            11732..12706
                     /locus_tag="PKM27_14330"
     CDS             11732..12706
                     /locus_tag="PKM27_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08493.1"
                     /translation="MKNRFLIPFFLLPFVSPVLAQSGSRQVKNPFMPPPGTSASIFAP
                     GMVSDEFGNRDFALAPDGLSFYYTLQYKNGLFSTILYSRKKKGKWTRPEVAGFSGRYS
                     DLEPAFSPDGRRLYFVSNRPLDPTGNKKDYNIWFVSLEEGRWSQPEALPFPVNTDANE
                     FYPSVSKNGNIYFTRVMEGQDEDIVLCRRQDNQYLPAVSLPGTINSTGAEFNAFVDPE
                     EAYVLFTAYRREGNLGQGDLYISYRDAGGNWSEAMNLGNKVNGPGLTYCPYVTADKKY
                     LFFSTARGAFAPPFPTAQSLDKLRTLFHSTQNGWDNIYWIGTEGIVKD"
     gene            complement(12894..14177)
                     /locus_tag="PKM27_14335"
     CDS             complement(12894..14177)
                     /locus_tag="PKM27_14335"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08494.1"
                     /translation="MKNKRTEERRAFLKQAGYASMGMMILPHLRRGVPPSDKVRIAHI
                     GLNGMGTNHLNWFQALPEVEIAALCDVDELHLAKAKSTLLSLQPDAKPDTYSDFRSIL
                     ERKDIDAITCATPDHWHAQIAIMAFEAGKDVYGEKPLSFNVREGQQMLKALNKHRRVF
                     QLGTQIHAGDNYHRVAEILQSGVLGRIHTVRLWKTGEPPVLELTPGPVPSTLNWDFWS
                     GPGPLRPYAPERCHLRYRYFLDYSGGIYQDFWCHIADIVWWSIQPKGLKSISSRGEKS
                     AGAGDTPRWIEVDYAFEGLNLYWTSTPPPVKGAENRHIGAYFEGEKGNLICDYNTREI
                     QIGGEYMNDLASVGQSIKRSPGHQQNFIDSVKSRQQPESNLAYVREMTLPMHLGLISW
                     RLGRRLEWNPLKEKFKNDPEANRLLKRKYRKGYDW"
     assembly_gap    14350..14449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14530..16143)
                     /locus_tag="PKM27_14340"
     CDS             complement(14530..16143)
                     /locus_tag="PKM27_14340"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNR08495.1"
                     /translation="MSKYVFITGGVTSSLGKGIIAASLAKLLQSRGFRVTIQKFDPYI
                     NVDPGTLNPYEHGECYVTNDGAETDLDLGHYERFLNTPMSQANNVTTGRIYQTVINKE
                     RAGDYLGKTVQVIPHITDEIKRRMRLQGDKGYDIIITEIGGTVGDIESLPYLESIRQL
                     RQELSDHDTVFLHLTLIPYLSAAKELKTKPTQHSVKELLTTGIQPDVLVCRTEHPINK
                     EIRQKLALFCNVERNSVIEAIDAESIYDVPLLMLKEGLDKIVLAKLHLKSKHEPELEL
                     WRNFLAKLKHPLHEVNIGLIGKYHELRDAYKSIYEAFVHAGAVNECHINIEAIHSESL
                     ENLDAIPERLQHLHGILIAPGFGERGIEGKYKAIEYARTQGVPFFGICLGLQSAIVEF
                     ARHVLHIRDAASTEVNPKTKNPVVDMMPEQKGITAKGGTMRLGSFACKIKKDSKVFKI
                     YGNDLIHERHRHRWEFNNKYLEEFESHGFKSVGVNPSTKLVEIMELKSHPWFVGVQFH
                     PELQSTVENPHPLFVSFIQAAIRFKMKEQ"
     gene            complement(16204..17457)
                     /locus_tag="PKM27_14345"
     CDS             complement(16204..17457)
                     /locus_tag="PKM27_14345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNR08496.1"
                     /translation="MSRLPSGKNDETVRAKIARLHRPLVLAVVSCLDAVFRNHKPADR
                     VLEKCFKDHPGFGKRDRSFIAEHVYDITRNYLLIASAFPQYVDFWEIFGAWLVIQGRD
                     LPDWREFDRLHQTRIGSRIPGLVKNPALKYSIPPELDAYGHRALGDRWYAEMAAMHQA
                     APLVIRVNTLKSNPMQVMDFFREQQVPLHLIDPSLPEALQIDQRINLFATRAFREGWI
                     EIQDYSSQQVAHFIRPDPGMRLIDACAGGGGKSLHLAARMANKGRIISLDIEEYKLKN
                     LKARAKRAGAGIIESRLIENNKIIKRLESSADAVLLDVPCSGSGVLRRNPDAKYRITP
                     DYIAQLVAKQKDILASYCKMVMPGGLLFYVTCSLFPEENEIQVHNFLTEHEGFELIED
                     RHLWPSECGYDSFYMAKIKRREGAA"
     gene            complement(18154..18762)
                     /locus_tag="PKM27_14350"
     CDS             complement(18154..18762)
                     /locus_tag="PKM27_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08497.1"
                     /translation="MQNDITGVWQLINSGDCNRNFLELSPFIDPYAKYGHKIYVSDSL
                     FYNPWMREEGLPFERRYKVKDKQIFLNGIGQILFTREDSILVLEKEGCKLSFIKKADV
                     ETLSEYSLANITFYVKDNDGSLVDSLNLQRTTSNAHIFRLAASIEIENLDKVFDEGVS
                     DTNEYKIILQLRNGKSFRITSYGKYETPFEVQTLIKYLLREN"
     gene            complement(18898..19101)
                     /locus_tag="PKM27_14355"
     CDS             complement(18898..19101)
                     /locus_tag="PKM27_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08498.1"
                     /translation="MNIEKIAGILFIIYGIYIAYETSKYHYPFNKETYYVYQFKDYFA
                     ALMAFILGLLLIFSNITFNELFD"
     gene            complement(19106..19519)
                     /locus_tag="PKM27_14360"
     CDS             complement(19106..19519)
                     /locus_tag="PKM27_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08499.1"
                     /translation="MAGGEMTEKVAENKPFTLVLGAASCNFNYGADGTKLSKRVKTGG
                     AINLVIVKDPASNILSIGKYVNGVEYPGTAIPATILEAVYLSEGRMHNRGGTSKREYI
                     LGGQLGNTLIAFCDFNGNGVIAAPGQILQEIKFDA"
BASE COUNT         4943 a         4906 c         4846 g         5088 t
ORIGIN      
        1 catggtccgc ttcaccgatt caaaataagc gatggaatcg tatgcctccc agaaaaagat
       61 gagggagatg aatatgcgtc ccattaaatc aatccagtct ttcacacgcg tcgggtttta
      121 tttgagtacg tattgaaggg cagcttgagc acagtgttgt ccatccattg ccgccgagac
      181 gataccgccg gcataacccg ctccttcccc gcatgggaaa agcgcttcac aatcgggatg
      241 catagatagt gaatttctgg gaatacgaac aggactgctg gttctgcttt ccgtggccac
      301 gaccaccgct tcatcggtga tatagccttt catttgtttt ccaaactgcc tgaggccggt
      361 gaccagggat ttaacaataa aggccggcag cacctggtgc accggcgccg accagattcc
      421 cgggatatag gatacctccg ggaggctgga ggacaactta tgatttaaaa aatcggttaa
      481 cctttgtgcg ggcgcccgct gggacccgtc ccccgccctg aatacggctt gctccacttc
      541 ctgctgaaac gtcaggcagc aaaacggatc ggcaggattt ttaaaatcag aagggaatac
      601 cgcggccacc atcccggaat tggcgaaacg gctgtctctt ctggaaaggg acattccatt
      661 gatcacgatc tccccgggcg cggtggccga cgggaccatc aggcccccgg ggcacatgca
      721 aaatgaaaag acccctctct cattgaaggt gcaggacagg gaataactgg ccgccggaag
      781 ataaggttcc cgcccggccc tccggtattg tatttgatcg atcagttgct gcggatgctc
      841 caccctcacc ccaagcgcaa aggctttggc atccagtctg agatttttcc gggcaagcaa
      901 atgataaatg tcccgggaag aatgccccgt ggccaggatg aatgcctcac cggcaaattc
      961 atcgccggag gcggtaatca ctgatctcat gcgcctttcc cggatgacaa aatccaccac
     1021 cctctgatta aaatgaactt cacccccgga ggctaggatg ctgttcctga tttgttccac
     1081 gatgccgggc agtttgttgg atccgatgtg tggatgcgca tcgatgagga tatcgggatc
     1141 tgcaccatgt tctacaaaaa ggcgtaaaat ctttcctata tccccccttt tcccggatcg
     1201 ggtataaagt ttaccgtcag aataggtccc ggctccgccc tctccaaaac aataattgga
     1261 atccgggttg acgatatggt cctgctggat ggcttttaag tccctgcgcc tggatttcac
     1321 atctttcccc cggtcgagta ctatgggttt cagcccgaga atcaggcatt ccagggcggc
     1381 aaaatatcct gcggggccgg cacccatgat caccaccgaa ggcgcggacc gaacatcccg
     1441 gaaacggctt ttgatcgtcg gttctgtggg taatccttta gcctcttcga gttctacctg
     1501 gtaccggtac accggatttc ttctggcgtc gagggaccgc ttgagcaccc gaaaaagcgg
     1561 caattcatgt acttctaaac ccaggtgccg ggccaggttt gtccgaaggg ctttatcctg
     1621 gtccggatca gcaggatcgg ttacgatttc gagccttgat tccatatcag gaaacttccg
     1681 ggagaatggc cctgcggatg cggacgaggt gcttcagcag gctttccatc ctgtccaggg
     1741 caagcatatt cgcgccatcg gatttggcta tggaaggatt gggatgggtt tctataaaca
     1801 aaccgtccgc tccggctgca atgcctgccg aagccatggt ttttataagt gcgggacgtc
     1861 ccccggtaac cccggaagcc tggttgggtt gctgcaatga atgggtcaca tccaggatga
     1921 ccggtacgcc gagcccctgc atgaccggga tggaacgcat atcgacgacc aggtcctggt
     1981 aaccaaaggt cgtgcccctt tcagtgagcc agacatttgg attgttccgg gcctggacct
     2041 tatccaccgc gaatttcatg gcctccggac ccatgaactg tcctttttta atattgatgg
     2101 ttttccctgt atccgcagcg gcttccagca aactggtctg gcggcataaa aaagcgggaa
     2161 tctgcaagac gtccacaaag ggtgcggcca ggatggcttc aggttcggta tgaatatccg
     2221 taagcaccgg aatgccaaaa tggtcccgga cctcttgcaa atattgcaag gcgacgacat
     2281 cgcccagccc ggtgaaggag tccttgctgg accggttggc tttacgataa gaagatttga
     2341 aaataaaagg gattcccagt tcacgggtga tccgggtcag atgatccgcc gtctcaaaga
     2401 tcaggtcccg gctttctatg acacagggtc ccgcgatcag aaaaaacagg gggctttccg
     2461 ggttgttaac aaggggcagg ttctgaaaat tcatcggaaa tgtttgctct tacatcagtt
     2521 taatacccag gcggtaggtt caatatacat gcggatgtcc ttcagggtca attccaggtc
     2581 ctcttcctca tagacataga tccccaggcg aacacaatct tcgctcactt tttcttcata
     2641 caaccggtac tgcatgtatt gtattttttc cttcatctgc cggatgttgc cttcccggca
     2701 aaaagtgccg gtgatgatga tccgttcctg gataagcggt gaagcttcgg ccacttcctc
     2761 ctcagggggc accgtatccg cttgatgagg ggcgatattg acctctttta aagggccggt
     2821 ggatcgggaa gtcgcctggg ttgcgctgat ctccggggaa gggatgctca gaaaataaaa
     2881 gaacaaaccg gccagggtga gggtaatcag cgcgatggcc agccaaatcc agatcctcgg
     2941 gtttttttca ggtacctgga cggtcagggg aggcagttgc aaaggagcaa attcgaataa
     3001 agcatccaga tgcggtccct tgagaaattc caggctgtcc gatccctgag ccggtttgag
     3061 ttgccccagg acacccagtt ccaggggcag ttgccgggca agggttgttt gaaatttcag
     3121 gaacaccgat tcgaggaaac gggaggcctc ctgcaaatcc acctcatgca actttgaaac
     3181 cagggaggcc atggtatgga agttgcccgg gttcccgggc acccattgaa ggctggccct
     3241 gggaggttca aaggattgcc tggatacgct ccaggaggct ggcgaataat caagaataaa
     3301 acttccccag gtaccgattt cagcttttcc atagcgtttc aaacactcca atatataagc
     3361 tgaaaaaccc atgttgaatc aggaagcaag gtacaaaaaa ataagccggt gcgaatcagg
     3421 cgatggatgg ccggtaaatc cgcggcacgg ctttaagcgt ggcggccgcc gtgcccaggg
     3481 tgggcgcaaa aaacaggccg acccccggaa tcatgatgat caggaaaaag gcaatgccgt
     3541 tgcccaggct gaagccaaag tttttacgga taaaatcgat gctcccattg atgctgaaat
     3601 aacggccgag ggtaaaatcg aggttgacat agccggaata catggcctgg atgaggaaaa
     3661 tggccggagt ggcgacgtaa cccataaagg ggatcagccc cacgatcagg agcccgatgg
     3721 taaagatcat ttcctttaaa aaattgcgca ggcagaattg cagactgcgg ccgtattctt
     3781 tgaggaccgt ttcatccgat tctttacggt aggaaatgga ataaccgatg accgcctcct
     3841 ccaccctcct ggacaagccg ttgagaatag gagaaagcag gaccatgatc acgtatttat
     3901 aaatcacgac cccggtggtc gctatgccca cgcccacgat ggccgaagaa attttggaca
     3961 gaatccggct gtccgaatcg gtgggcaata tggtataaat aaggtccttc aggttatcgg
     4021 accaatacca ggccgtgaat cccaccgtca ggccgtaaaa caaactgatc agccccggga
     4081 taatgaggta catccatagt tttttatcga ctatgatggt ccaggcttcc caatacgcct
     4141 taaaagatgt taccagatcg cgaagcataa aaatagaacg ggcaatttac caaattcgtt
     4201 agcaggaatt tccaaacgaa atgtaaatgt ccgaatgatc cggtcctaaa ctatgttaaa
     4261 ataaaattta atatgtcacg ccggcatccg ggccagggct tgacggtcat ccggtaaagc
     4321 ctgtgatcag gtcgtggcct tttttctgcg ggtcttgaac aattcggtga tgtccttttc
     4381 cttcatgtac tccagttcgg cgaaatgggc cgtcacaaat tttttgatct cctggtagtc
     4441 cttgcccctt ttatcgttgt atttcaaaag cagtttgtgg atgaactgct gattgaggcc
     4501 ttgcacttcc tggttgaatt tttcattgga gaaaatcttc atataggtat taaagatcct
     4561 cgacatcgaa aaatagtcat ggtattcgct ttctcccagg ttttcaagaa gtcctttaaa
     4621 ataaccgagg atggtatcgc gaacggtcgt attgatcacc gagaggcctt cgttggtttc
     4681 atagaaggaa cggacggcct caatggtatc ctcgtgcata tagcccttgg tatgaatgag
     4741 atccgccagt tggtaatacc tgctgacatc gtcctggatg tcctggtcca gcaagccggc
     4801 cgcgttccgg tcgacattgg catcaaactt gatccctgcc aggtgcatgc tgtgcaggaa
     4861 attaaaatat tgctcgctga aatccgggga attgcgccgg ttttcattca gcagcctgcg
     4921 cagcaattcc ctgtcgtttc cttccaggtg gaaaaagttg gtgacgataa agacgattga
     4981 ccaaaattca gagaggtggt ataatttttc atttttaagc aggtcggcga ggtaagggat
     5041 caggctttca ttgttcctga aacggctgac ccggtgcagg agaaattcct ggagcgggat
     5101 ataaaggctt ttaaggtcct ccggggtctc gggaaactcg gccgaatgat aaggagcccc
     5161 ttcaaattgc ctgctgtatt tctttaaagc gacggctctt tcctgctcgt ccgcgatctt
     5221 attggtgcgg atattctcaa aatattgcct gctccttttg ttttcaatgt gttcgatcag
     5281 gttggatacc cagatatcgc tggaaaggga aaagccgatt tgaagggtct gtcccgtttt
     5341 attcttcagc acctcaaaat tgttatgggt ggccagggca tgcacggcca gaccaaactg
     5401 gtcctggggc ctcctgtact ttacctgttc gttgacatcg ccccgcaata ccgtatagcc
     5461 cagtatcttc ggaaggtaaa ggccttcaaa tttctcatcc agcaggtgct gttccaggtc
     5521 tatcaactgc aggggctttt cttcgcccac ttcggtataa agggcgtgta tttctggctc
     5581 aaattcatca cgcagggcct gaaaggcctg ttcggtctct tcttcgagat aggattgcag
     5641 gtggtcggaa gattggatct gttcacggat ggcgtctacc cggtctgcat atttggaatc
     5701 cagtttttcc atggatcgat ttttttttaa aaataaaatc tggtaaaacg aaaaagcgct
     5761 ttaccagatt gaaaaataat attctgtcgg tttaaggcag gattattcct ggggagccga
     5821 ttcttcggca ggggcttctg gctgggcttt tttcctggat ggtttggctt tgccgaaaac
     5881 catttcatgg aaagccttga cctcagcggc gcttttgatc acctcctggg cattggcccg
     5941 gtctttgtcc tggatgaatg cctggtattt ctgttcggct tcttcgatgg tcatggctcc
     6001 ttttttgacc ccgcgcatca ggtgcttttt ataaaggacc cctttgaatt tcaggatatt
     6061 cctgaccgtg tcggtaggct gcgcgccctt gcccagccaa tccagggctt tatcccggtt
     6121 gatgtcaatg gttgccggtt tggtgatcgg gttgtaggta ccgatcaact cgatgaattt
     6181 tccgtcccgg ggcgcccggg catccgctac cacaatgtgg taatacggcc tctttttccg
     6241 gccgtgacgt tgaagtctga tttttactgc cataaaatga taaacattta gtagtacttg
     6301 ttaaaaaatg gatgcaaaag taaggatatt atctgtaatt caggaagccc accattaatt
     6361 ccggcatatt ttaatggaag ccgatgcggc attttatatt tatcctcctg gatgtcagat
     6421 aattgggtat ggcgtattgt acattataaa tggacttgaa ccaggtgttg gacgccacgt
     6481 tctgcacctt gagcaggtta aaaatctcgg cgctgatcca ggcattttcc gtaaaacgga
     6541 aaagatgttt agacctcctc gcccgccagc ttttatccca gatgaggtag gaaaagccca
     6601 gatcgacccg gtgataagcg ggatagcggt aagtattccg gaatatccgg ttgttcccgg
     6661 gaattccaaa aggctgtccc gttccgaccg tcaggttcag gttaactttt aaattctcat
     6721 tgccgggcaa atagtcctgg aaaagggcgc tgaaggtcag aaactggttg gtgggccttg
     6781 gcacatcctt tacgtttttc ccctcggctt cgcctttctc ccttctttgg tgctgcaaac
     6841 cgtctatgga ttccctggcc cgcaggaaag aaaggttaaa ccaggattcg gctccccgca
     6901 cgaattcccc gttgagcctc aggtccaggc cggtgatata accggaggca tcgttcatgc
     6961 cggaataccg gatcttgaca ttatcaatat cgtagatgac ctggtcccac aattttttgt
     7021 aatagacttc cccaaccagg cggtaaggaa ccggttgttt gattccccgg gtaaaatccc
     7081 aggtaaaacc ggccagcagg tgggcggact tctgggactg caggtcccgg ttgaccaatc
     7141 cctccggggt cctcagttcc cggtaaaagg gaggttgata atacaagccg cccgcaaaac
     7201 gaagggtgat gtctttcttc cattggccgg gcttgtataa tacctgggcc ctcggattga
     7261 tataatattc cttattgagg gtccagtagg ccatgcggaa cccgccggaa atccgcagtt
     7321 cgcccagccc atcccgctgc atggtgtatt catcctggaa ataggcggaa aaacgggtgg
     7381 attccaattc attccgggtt ttgagcacat ttttgatgac cacgtcctgc tccgtatggg
     7441 gaagggtgta cagggcggaa tccagccgct cccagtcgtt gaggcggtcc agaatttcct
     7501 cccgctgggc cctcaggccc cattggaaaa aatgggtttt ttcacggctg gccccgggac
     7561 tgtactcata gccccctttt aactcaagat tgcgaacctg gagataaagc aggttgcgcg
     7621 cgtataaatg ctggaccccg ttgccgataa gggccagctc cttgccctgg ttttcagatc
     7681 caaggccggt ctcaataagg gaaagccggt aatcgctgat gatgtcgaag gattcttttt
     7741 cgaggctttg gtatgcgctt cccaaaaact tgatgaagag cgggtttttt ttcctttcgg
     7801 ggataaaagt caatgaaagg cctcccatgc ccgttctgaa atggtcaatc tcctggcctt
     7861 cgaaatcagc gcttaatttt aatgaaaaat caatcaaacc cgttgccgta ctcctggaca
     7921 agggcacaaa acggaagatg gaagaattgt aatttcccag cgcgcccagt tgccagtttt
     7981 tattgatatc gtaagtcagg taagcctgga catcggcaaa atcgggcacg tattcccctt
     8041 ttaattgaag ggaattgagc aggtaccggt ttgttttgta gcgggcgcca accaggtagg
     8101 tgaatttccg gtagctgctc ctgccggcct gatggcttcc ctctaggtgg cccgtggcgc
     8161 ccaggaggga catggacacg gaacccttca ccgaatccgg cctccggtaa tgaatgtcca
     8221 gtaccgatga aagtttgtcc ccataacggg cttcaaagcc cccggaggaa aaagacatgt
     8281 cccggatcag gtcggcattg ggaaaggtca atccttcctg ctgcccgctc cggaccagct
     8341 gggggcggta aacttcagag tcgttgacga aaaccaggtt ttcatcataa ttcccacccc
     8401 gcacattata ctgactgctg agttcaccgc ctgtgccgct ggaggtaccc agggcaatat
     8461 gaggcaggat gctctcgaaa ttgcccgtga ccgaagggat cagtttgagg ttttccaggt
     8521 tttcccggat catgccccgg ttccggacat tgctttcccg gatgattacc tcaaggctgg
     8581 aagattccag gaccaggtcg atatcgatgc ccaggactgt tccggctgca atggcactga
     8641 ccggatagga tgcgttttta taacctatcc ggctgacttc gagaagcaag ggaatattgg
     8701 cgggtacctg gagcgaatac cggccgtttt catcggtttc ggtgctgaga ttttgggcac
     8761 ggacgaaaac cgtcgccagg tccaccgcct ggttattttc aacatccctg acctggccgg
     8821 tgatcgtcgc gacaggctgt gcgtggacca tgctgaggca gagcagggtc agcatgcagg
     8881 tataaaggat tttacccatg cctgactaaa cgaaaaaagt ggatttggaa aattgcctga
     8941 ggcgatgaat gacggaagcg gggtccggat ccgaaaatac cgaactgcct gccaccaaca
     9001 catccacccc cgccttgacc acgggttccg cattttgaag gccgatgccc ccgtcgattt
     9061 ccaccagggg attcaggttt tcttccgcca tcatttgttt gaggcgcctg agtttcggga
     9121 tggactggta gataaatttc tgaccgccat acccgggatt gaccgacatg atcaggacca
     9181 ggtccaggat ttcgaggaca tcgcgcagtg actccaccgg ggtgtggggg ttgaccgcca
     9241 ccccggcttt ggctcccagg gtgcgaatat attccagggt ccggtgtagg ttggggcagg
     9301 tttcgtaatg caccgtaatc aagtcggctc cggcgtcacg aaatgcctcg agatgccggt
     9361 ccggttccac gatcatgagg tgcacatcca agggtttggc cgttcttttc cggatgcttt
     9421 ggatgaccgg gatcccaaaa gaaatattgg gtacaaaaac gccgtccatg atgtccaggt
     9481 ggatccagtc cgcctcgctt tgatcaagca ggtcgacggt cttgcccaac tcagtgaaat
     9541 cggtattcag gatggaaggc gctacgagga cttggtcttt cattgaaata tttctatctg
     9601 ttcccggctg agcagcgttt tatgattaaa ataaagataa agttgtgcca cggtgatggc
     9661 atccttttgg caatagtcca cgatccgggc caggttattt tcccgccagt acaccgggcc
     9721 cacctggctg ccgtcgatat cgtccttggg gctttcgatg cccagcacat tggcgagcag
     9781 gttgagggaa gtatagtgtt tgacgtctcc gaatttccag tattgcatgg tatcgatatg
     9841 ggtggattcc cagggctttt taccgctgag gttgaggatc ctggggaagg ggatctgatg
     9901 gaccatcatc ctccggcaaa ggtaagggac atcaaattca cggatattat gaccgcagat
     9961 ccggtgttca tcccggtaga accgttttcg gagcatttcg gcaaattcca ggagcacctc
    10021 tttttcatca tggccaaaaa aggacttcac cttcagtttc aggtcctggc cttccttaag
    10081 atcaaagcgg gccaccgaaa tgcagatgat tttcccgaat tccgcgaaga tggctgcctt
    10141 ctgggtgtaa aattcactga cttctgcatc agtcatgggc tgatcgtagg accgggtgaa
    10201 ggcattggcc ttgagtttcc acaacatctt catagtttcc ggcagttcac tgaactccgc
    10261 ttttccggac accgtctcga tatcaaagaa taaaatatcc tgggctttgg tattttccat
    10321 tcacataaaa tataagcaaa aatgctcaaa attcatcaat tgtccgccgg aggcggtcca
    10381 ccggacaggt ccggaataca aggcaaaaat tatttcccgc cggctggaac accttatgta
    10441 tacataccgc cggattccac tgatttggtc aaaaagcctg caaaaccctc acggtcatgg
    10501 aaataggggc catggattgt taacaagcca ttatgtaaat tttacagttc aaaatactaa
    10561 gcggggggca gggctgcgtt aatacctcat aactttgtaa tactaaaaat ctaaaaacat
    10621 gtcagactac aattccaaca acaccgctaa aaaaagcaat tacttgccga tccttattgg
    10681 tttgttgatt gcttctgtag caggaaacgc ttatttgttt ttcaataagc agaaacaaca
    10741 ggagattatt gtaaacacca ccaccaaact taccgaatcc gaaaggctta aagccgagct
    10801 ggaaaaacaa tactatgaag ccctacagga cctggagaaa caacgcaccg acaacaaggc
    10861 ccttaacgaa ctgatagaaa gccagaaggt tgaactgaaa acccaaaagg accggatcgc
    10921 ccgcatgctg ggtgattcca aaaatctcgc ctccgccaaa aaagaaatgg ccaatctccg
    10981 cacccaggtc gatggatacc tcgcagagat caacaagctg aaagaagaga atgccaccct
    11041 cgcgaccagc aacaatatgc tgaccgagga aaagaaatcc ctcaccacca acctggagga
    11101 gaccaaaaaa gtaaacgatg aattgaccac ggccaaagcc gtcctgatca gcgaaaagga
    11161 aaaactcgct tccgaaaaca ccacccttgt taaaaaagtg aatatcgcct ccgtggttaa
    11221 agtcaacaac atcgacatca gcggttaccg catcaaaagc agcggcaagg aagtggaccg
    11281 caataaagcc aaagcaatcg acggactccg catctgtttt gatgtgactg aaaataccat
    11341 taccgaatcc ggtgttgaac aattctatgt acgggtgatc aacccgaacg gcgaaaccca
    11401 ggccctggaa aaccttggct ccggcgtact ggtgaatcag gccaacaacg aagagatcaa
    11461 gtacacctct cttaaaggcg tgaagtatga aaacaaaccg cagaatacct gcatgaactg
    11521 gcagccaaac attcctttcc aggaaggaac ctataccgtg gaagtgtaca ataaaggcta
    11581 tttgtccggt acgaccacct tccaactgaa gtagagtaaa cgtaactttt tcatagcatt
    11641 ttttaatggt taagcctgcc gtaacccctg cggcgggctt tttttttgct gcccccgggg
    11701 tagcaccctg gcctttttcg tatctatcgg catgaaaaac cgattcctga tccctttctt
    11761 cctcctcccc tttgtttcac ccgtacttgc ccagtccggt agccggcaag tcaaaaaccc
    11821 gtttatgccc ccacccggca cctcggcctc gatcttcgca ccgggaatgg tatcggacga
    11881 attcggcaat cgcgattttg ccctggctcc ggatggcctt tccttttatt acacccttca
    11941 gtataaaaac ggcttgttca gcaccattct ttattcaagg aaaaagaaag gaaaatggac
    12001 ccggcctgaa gtagccggat tcagcggccg gtattccgac ctcgaacccg ccttttcacc
    12061 cgatggccga agactctatt ttgtctcaaa ccggccactg gatcctaccg gcaataaaaa
    12121 ggattataat atctggtttg tatcgctgga ggaaggccgc tggagccaac cggaagccct
    12181 cccctttccc gtaaataccg atgccaatga attttacccc tccgtttcca aaaatggcaa
    12241 tatctatttt acaagggtca tggaaggcca ggatgaagac atcgtactct gccgccggca
    12301 agacaaccaa tatcttcccg cagtcagcct gcccgggacc atcaacagca cgggagctga
    12361 atttaatgcc ttcgtggacc ccgaggaggc ctatgtcctt tttaccgctt accgtcgcga
    12421 aggcaacctt ggtcagggtg acctttatat ttcgtacagg gatgcaggcg gtaactggag
    12481 cgaggccatg aatttgggca acaaggtcaa tggccccggt ctgacctatt gcccctatgt
    12541 gacggcggat aaaaaatacc tgtttttcag cacggcccgc ggtgctttcg ctcccccttt
    12601 ccccactgcc caatctctgg ataagctgag aactttattt cacagcactc aaaatggatg
    12661 ggacaatatt tattggatcg gtacggaagg gatagttaag gattagtttg ctagtttgct
    12721 agtttgctag tttgttagtt tgctagtttg ctagtttgct agtttgctag ttagtaagtt
    12781 ggagacctca aacctcaaac ctcaaacctc taacctctga cctctgacct ctgacctcta
    12841 acctctaacc tctgacctct gacctctaac ctctaacctc caacctccac ttctcaccaa
    12901 tcatacccct ttctgtattt ccgttttaac agccggttgg cttcagggtc attcttgaat
    12961 ttttccttca ggggattcca ttccaggcgg cgtccgagcc tccaggagat caggcccagg
    13021 tgcatgggca gggtcatttc ccgcacgtag gccaggttgg attccggttg ctgtcttgat
    13081 tttacggaat cgataaagtt ttgctgatgc cccggggacc ttttaatgct ttgtccgacg
    13141 gaagccaggt cattcatgta ttcccctccg atttgaatct cccgggtatt gtaatcgcaa
    13201 atcaaattgc ctttttcccc ttcgaaataa gctccgatat gtcggttttc ggcgcccttg
    13261 accggcggcg gggtcgaggt ccagtaaagg ttcaagcctt cgaaggcata gtccacttca
    13321 atccacctgg gggtatcccc cgccccggct gatttttcac cgcgggagct tatggatttc
    13381 aggcctttgg gctggatgga ccaccagacg atatcggcga tatgacacca gaagtcctgg
    13441 tatatgcccc ctgaataatc cagaaaataa cggtaacgga gatggcatct ctccggagca
    13501 taaggcctca gagggcccgg gcctgaccag aaatcccaat tcagggtgga agggaccggt
    13561 ccgggagtca attcaagcac ggggggctcc ccggtcttcc ataagcgtac cgtatgaatc
    13621 ctgcccagga ccccggattg caggatttcg gcaacccggt ggtaattgtc cccggcatgg
    13681 atctgggtgc ccagctggaa tacccggcgg tgcttattca gggctttgag catttgttgc
    13741 ccttcccgca cattgaagga caagggtttt tctccgtaaa cgtctttccc ggcttcgaaa
    13801 gccatgatcg cgatctgggc atgccaatga tcgggcgtgg cgcaggtgat ggcgtcgatg
    13861 tcttttcgtt caaggatgga ccggaaatcg gaataggtat ccggttttgc gtccggctgg
    13921 agtgaaagca gggtgctttt tgccttggcc aggtgcaatt catccacatc gcaaagggcg
    13981 gctatctcca cttcaggcaa ggcctgaaac cagttcaggt ggttggtccc catgccgttg
    14041 aggccgatat gcgcaattct cactttgtca ctgggtggga caccccttct taaatgggga
    14101 aggatcatca tccccatgga ggcatagccg gcctgtttta aaaatgcccg gcgctcctct
    14161 gtccgcttgt ttttcatgga tcaatcattt aacgatccca agataatcaa atcgaacttg
    14221 ctgactaacc aacttaccaa cttaccaact taccaactag ccaactagcc aactagccaa
    14281 ctagccaact agccaactag ataactagat aactagataa ctagataact agataactag
    14341 ataactagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ataactagat
    14461 aactagataa ctagataact agataactag ataactagat aactagataa ctttcaaact
    14521 ttctaactgc tattgctcct tcatcttaaa ccggatggca gcctgtatga acgacacaaa
    14581 cagggggtgc ggattttcca ccgtgctttg cagttccggg tgaaattgca cgccgacaaa
    14641 ccagggatgg gacttcaatt ccatgatttc caccagcttg gtggaggggt tgacgcctac
    14701 ggacttgaac ccgtgggatt cgaattcctc caggtatttg ttgttgaatt cccagcggtg
    14761 gcggtgcctt tcatgaatga ggtcgttgcc gtaaatttta aaaacctttg aatccttttt
    14821 gatcttgcag gcaaaggagc ccagcctcat ggttccgccc ttggcggtaa tgcctttttg
    14881 ctcgggcatc atgtcgacca cggggttttt ggttttggga ttgacttcgg tggaagccgc
    14941 atcgcggata tgcaagacat gcctggcgaa ttccacgatg gcggactgca gtcccaggca
    15001 gatgccgaag aacggaacgc cctgggtccg ggcatattcg atggccttgt acttgccctc
    15061 gatcccccgc tcgccaaaac cgggagcgat caggattccg tgcaggtgct gcagccgttc
    15121 cgggatggcg tccaggtttt caagggattc ggagtggatg gcttctatgt taatatggca
    15181 ttcattgacc gcgccggcat gcacaaaggc ttcataaatg gatttatagg cgtcgcgcag
    15241 ttcgtggtat ttgccgatca gaccgatatt gacttcgtgc agggggtgtt tgagtttcgc
    15301 aagaaaattt ctccacaatt ccagctcagg ctcgtgtttt gatttgaggt ggagtttggc
    15361 caggacgatt ttgtcgagtc cttctttcaa catcagaagc gggacgtcgt aaatggattc
    15421 ggcgtcaatg gcttcgatta cggagtttct ttccacgtta caaaacaggg ccagtttctg
    15481 acggatctcc ttgttgatgg gatgttcggt gcggcagacc aggacatcgg gttgaatccc
    15541 cgtagtcagc aattccttga cgctgtgctg ggtgggtttg gttttgagtt ctttggccgc
    15601 ggaaagatat gggatgaggg taaggtggag gaaaaccgta tcgtgatccg acagttcctg
    15661 cctcaactgg cgaatggatt cgagataagg cagggactcg atgtcgccca cggtgccgcc
    15721 gatttccgtg atgatgatgt cgtatccctt gtctccctgc aggcgcatgc ggcgtttgat
    15781 ctcgtcagtg atatggggga tcacctgcac ggttttcccg aggtaatcac ccgccctttc
    15841 cttgttgatc accgtctggt atatccggcc cgtggtcacg ttgttggcct ggctcatcgg
    15901 cgtattcagg aatcgttcat aatgcccgag gtccaggtcg gtttcggcgc cgtcgttggt
    15961 gacataacat tcgccgtgct cgtaaggatt gagggtcccg gggtccacgt tgatataagg
    16021 gtcaaacttc tggatggtta ccctgaagcc cctggattgc aataatttcg caagggaagc
    16081 ggctatgatc cctttcccca aagaagaggt caccccaccc gtaataaaaa catatttgct
    16141 catagccccg gtttgaatct tttactacgg gtcgtaaagg taaaaaataa tcccggccaa
    16201 tccctatgct gccccttccc ttcttttgat tttggccata taaaaactgt cgtagccgca
    16261 ttcggagggc cagagatgcc tgtcctcgat taattcaaag ccctcatgct ccgtcagaaa
    16321 gttatggacc tggatttcat tttcttccgg gaaaaggctg caggtgacgt aaaacaaaag
    16381 gccgccgggc attaccatct tgcaatagga ggcaaggata tccttttgtt tggctaccag
    16441 ttgggcgata taatccggag tgattcggta tttggcatca gggttgcggc gcaggacccc
    16501 cgaaccggaa caaggcacat cgagcagcac cgcatccgca ctggactcca gtcttttgat
    16561 gattttattg ttctcgatca ggcggctctc aatgatccct gctcccgccc tttttgcccg
    16621 ggctttcagg ttttttaatt tgtattcctc gatgtccagg ctgatgatcc ggcctttgtt
    16681 ggccatccgg gccgccaggt ggagggactt gcctcccccg cccgcacagg catcgatcag
    16741 cctcatgccg ggatcaggtc tgataaaatg ggcgacctgc tgggaggaat aatcctgaat
    16801 ttcgatccag ccttcccgga aagcccgggt ggcgaagagg ttgatccgtt gatcgatttg
    16861 aagggcttcg ggcagggaag gatcgatcag gtgaaggggg acctgttgtt ccctgaaaaa
    16921 atccatgacc tgcataggat tgcttttcag ggtattcact cttatgacca ggggtgcggc
    16981 ctggtgcatg gcggccattt cggcatacca gcggtccccg agggcccggt ggccgtaggc
    17041 atccagttcc ggtgggatgg aatatttcag ggcggggttt ttgaccaatc cggggatccg
    17101 ggaaccgatc ctggtctgat ggagtcgatc gaattccctc cagtccggca aatcccgtcc
    17161 ctggatgacg agccaggctc caaagatctc ccagaaatca acatattgcg gaaatgcgga
    17221 agcgatcagc agataattcc tggtgatgtc gtaaacatgt tccgcgataa aactccggtc
    17281 cctcttacca aatccgggat gatccttaaa acacttttcc aggaccctgt ccgccggttt
    17341 gtggttccgg aatacggcat ccaggcagga aaccacagcc aggaccagcg gacgatgcag
    17401 ccgggcgatt ttggcacgga ccgtttcatc gttttttcct gaaggaagcc tggacatcag
    17461 gccgcaaggt acacctgtcc atttaaactc agaagaaagg cccctcccgg actgcataaa
    17521 ataaaaaagg attgcccgac gaatcggcca atccggtatg aaaaagaaaa atcaagacgg
    17581 aatttttatc ctccccaatc caagttaaag accagtccct ttggccggtc catgcttcaa
    17641 ttacccttta cactaaatta attgaccggt ggaggataaa aattccgtct ttcaatgata
    17701 caaagatgaa gccttgccag ggaccattga atgatccggg tcaaagatga aaatgaatac
    17761 ggtcaggaaa ggattaatat aatatactgc attatatgga tataaaaaat ttatattcta
    17821 tttttaatag atatcaatat aatatcttga tatctcctga ccgcggtcag gtttcaggcc
    17881 ttgcccaagg acgccgggga attccggcga gctgtttaaa gtcgatcaag ggaggaaatt
    17941 ttacctacct gagcagttgc cattagctat gagcaggttg aggaaatggt gcacttctta
    18001 gatgttgcat agaagcgacc tgttttctga gactgaataa gctccatgtc tccctggagg
    18061 atgaggacca caaagggttc tctctcctga ttcgtaaata taacacatac tttctatgat
    18121 cctgctcatt cgtcacatcg aatgctttcc catttaattt tctctaagca aatattttat
    18181 taaagtctgt acttcaaagg gagtttcata tttcccataa gatgttattc gaaatgactt
    18241 accatttctg agttgtaaaa ttattttata ctcatttgta tctgaaacac cttcatcgaa
    18301 gaccttatca agattttcaa tttcaatact tgcagctaac ctgaaaatat gggcattgga
    18361 tgttgtcctt tgcaaattga gagagtcaac taaagatcca tcattatcct ttacataaaa
    18421 tgtaatgttt gccaaactgt attcagataa tgtttctaca tccgcttttt taataaatga
    18481 gagtttacat ccttcctttt ctagtactaa tatcgagtct tctcttgtaa acagtatttg
    18541 accaatacca tttaaaaata tttgtttgtc tttaacctta tatcttcttt caaaaggcaa
    18601 cccttcttct ctcatccatg gattgtagaa caatgaatcg gatacgtata ttttatgccc
    18661 atacttcgca taagggtcta taaaaggaga taactctaaa aagttcctat tacaatcccc
    18721 gctgtttatt aactgccaaa ccccggttat atcgttttgc actacactac atttcaaact
    18781 taaaaagctc aaagagaata aacctaagat tactattaat ttaatacaaa aattcattct
    18841 gaatagctta atagtcaatt gcctttacgc catttttaag ccaaaaattt aattcaatta
    18901 atcaaaaagt tcattaaaag ttatattgct gaaaattaat aataacccta aaataaaagc
    18961 cattaatgct gcaaaataat ctttgaactg ataaacataa tatgtttctt tattgaaagg
    19021 ataatgatat ttggaggttt cataagcaat ataaattcca tatataatga aaagaatacc
    19081 tgcaattttt tcgatattca taacactaag catcaaattt aatttcctga aggatctgtc
    19141 caggagcagc tataacccca tttccattga aatcacaaaa ggcaataagg gtgttaccca
    19201 gttggcctcc aaggatatat tctcgcttac tggtccctcc cctattatgc atcctacctt
    19261 ctgataaata gaccgcttca aggatagttg ccgggattgc tgttcccggg tattccactc
    19321 cattcacata cttcccaatg ctcaatatgt tcgatgcggg gtctttgact attaccaagt
    19381 tgatagctcc tcctgttttt accctcttac tcaatttggt cccatcagca ccatagttga
    19441 aattgcacga cgccgcccca agaaccagcg taaagggttt gttttccgca actttttcag
    19501 tcatttcgcc accagccata aggttctgat caaattggtt taccgactat cgaagattca
    19561 ggtgtccctt caggatgttc ctgtccaaga tagttaaaat tctaacttga tgccaaggga
    19621 acacgaggta agaatcaggt ttgggatcaa actccaacta aggaaaaaat aaagcctggc
    19681 aatctttctt tatctggaat aaacccgact tatggttcaa ttttgagctt taaacttgtt
    19741 tcaaacgatg gccttaaata gtcaaaaatg tttgacaggt aaaataaaaa accctctaaa
    19801 tcaatgactt agagggtaat tcagtagccc cgacaggaat cgaacctgta tcaaaagttt
    19861 aggaaacttc tattctatcc att
//
LOCUS       DAOQXT010000089        19795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3102_length_19795_cov_5.951925, whole genome shotgun sequence.
ACCESSION   DAOQXT010000089 DAOQXT010000000
VERSION     DAOQXT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19795
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3102_length_19795_cov_5.951925"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..321)
                     /gene="trxA"
                     /locus_tag="PKM27_14365"
     CDS             complement(1..321)
                     /gene="trxA"
                     /locus_tag="PKM27_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNR08500.1"
                     /translation="MAQTLTDGNFKTEVLDKQGVALIDFWAEWCGPCRMIGPIIEELS
                     AEYKDKATIGKLNVDDNAEVTMEYGVRSIPTLLFFKNGKLVDRHVGATSKKVLTDKLE
                     ALMS"
     gene            405..1433
                     /locus_tag="PKM27_14370"
     CDS             405..1433
                     /locus_tag="PKM27_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HNR08501.1"
                     /translation="MILRSRHKITGIVVFSFISVLAFFLSPGLLEKVYGGFIFPLVRV
                     IFSILIWIPFPLIYLVLFSLLVWLALLFRGKGSVKNKALRSGALTLSLIFLFYWLWAY
                     NYKRIPFGARFPVTLASVDSQALREELQFAAQFLLENSPGHRSYPSIKETEGMIRPWV
                     DSVCRAYGYRTPGMIRVKALYPKGLLLRIRTAGFYFPHGGEAYIDPGLHELQVPFTMA
                     HEMGHGFGVADEGACNFIALMALHRHPDSMMRYSGAMGYFRYVAGAYRFYFPEDYQQF
                     RDQLPLQVQDDLNQINARLLKYPDIFPKLRDRIYDRYLKMQGIDEGLASYDRIIDMVR
                     EARRRGVW"
     gene            complement(1595..2578)
                     /locus_tag="PKM27_14375"
     CDS             complement(1595..2578)
                     /locus_tag="PKM27_14375"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNR08502.1"
                     /translation="MKKKIAFVTGGYSGEAVISYESAKTIEAHVDRARYDLYRIDIRR
                     DGWFYNPAQGIEVPMDKNDFSFTLDGQKTTFEAVLCGIHGTPGEDGKLQGYFDLLGLP
                     YTACGLASSAITFNKRYTVAVAAFSGIPVSRSLHFFKGDPVDPAQVFGPLQPPLFVKP
                     NNGGSSIGMSKINSPEELAPALDKAFKEDDEVIVEEFISGTELTIGVYRQGKDIVTLP
                     ITEIRSKKEFFDYEAKYTTGMAEEITPAPVSPAIRELIQNQAKKLYHIFNCKGVVRID
                     FIYNEASGKAFMLEINTVPGQSANSIVPQQIRAAGNTLQEFYTSLIESCLA"
     gene            complement(2648..3271)
                     /gene="mqnB"
                     /locus_tag="PKM27_14380"
     CDS             complement(2648..3271)
                     /gene="mqnB"
                     /locus_tag="PKM27_14380"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HNR08503.1"
                     /translation="MTKILVCAATALELEALGPFAEGVETLVTGAGSPSTAFFLAAYF
                     SKKIPGLAIQAGITGSFRPEIGIGQVVQVREDYFADLGAQDRDGSFIPLDTLIREKNN
                     PFQAYRLEPPDRYIMSGVLEVRAVTVNRVSGRQEEIDQMIKRLQPDIESMEGAAFFAS
                     CMVHRVPCLQLRAVSNRVEPRNRESWDIPLALNRLQESLARLQEILF"
     gene            complement(3271..3966)
                     /locus_tag="PKM27_14385"
     CDS             complement(3271..3966)
                     /locus_tag="PKM27_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08504.1"
                     /translation="MSNSSVLGYLHQTSFSAHAQSRFTGTGIRAGSIQAILPSGTHSA
                     FGLNMEWYGLPEYLRQLVRMGYGRALSPNLAIGLSFHYLGLHVSQAEPAANLGFDLGF
                     MIKINGDLGIGYSSYNPLYRGKGSKTSSPGANLAGLSYQLSEGVVLYAEWEKTGDRPW
                     KSHLALDYRVVPVLSLRMGFTANPAGLGAGIGLQPDPRLAIDISFDFHPALGISPALS
                     LRYIFVPKENGTD"
     gene            4239..5168
                     /locus_tag="PKM27_14390"
     CDS             4239..5168
                     /locus_tag="PKM27_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNR08505.1"
                     /translation="MAKFRSHYAGSGGKRGRGKSFLFRTVLYGMVLLYLLYALARFIQ
                     KADWKLPGKAEDRVESMGALDSLDFFPAVSTGNQLVIHRHFALSYSEKHEQAEWVAYE
                     LTVDELNSARQERFDYFSPDHKITTRSAVHRDYSGSGYTRGHLAPAADMAHDPVAAEE
                     CFFMSNISPQEKAFNNGIWRELEEQVRDWARKNRRLYVVTGPILNQGIRGKIGKNRVS
                     VPSLFYKVLLDYTPPDFKGIAFLLPNERSERKLQEYAYPIDSVEALTGIDFFTKLEAS
                     GEMESLENSINLQAWPFSQERFKERVQVWNKQE"
     gene            complement(5220..5720)
                     /locus_tag="PKM27_14395"
     CDS             complement(5220..5720)
                     /locus_tag="PKM27_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08506.1"
                     /translation="MPSKRTTHRSSAGKKLYAVRDASGKFKDIQTFERSHRADLAKKS
                     KQEQAAKAAKAAKPSKTTKAVAKAKPKKAAKPVAKKAVAKKAAKPAAKKAVAKKAVAK
                     KPVAKKAAKPAAKKAAPKKAVAKKAAKPAAKKPVAKKKAPAKKVTKPAVPAPAPVTPP
                     VTGSSN"
     gene            5871..7445
                     /locus_tag="PKM27_14400"
     CDS             5871..7445
                     /locus_tag="PKM27_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNR08507.1"
                     /translation="MQDLSSRFLESIQKGYSFKGDTLIMGVPMYNKQVVTGAFIRLPL
                     STLNRHGLIAGATGTGKTKSLQVLAENLSAHGVPCLLMDIKGDLSGLAMPGTSNAKIE
                     ERAAKTGLPFIPSASPVELLSLSGEKGIRLRATVSEFGPVLFSKILGLNETQSSIMAL
                     VFKYCDDQDLPLLDLKDMKKVLSFLSNEGRADMEKEYGRMSAASVGTILRNIIELEQQ
                     GAETFFGERSFDVNDLVRRDEKGRGILSILRLTDIQNKPKLFSTFMLQLLAEIYASFP
                     EAGDLDKPKLCLFIDEAHLIFDEASDVLLDQIETIIKLIRSKGVGVYFITQSPTDIPA
                     AVLSQLGLKIQHALRAFTAADRKAIKEAAENYPLTEFYDTDQLLTELGIGEAFITALN
                     ENGIPTPLAHTMMRPPVSRMDILEPGEIDRLVSQSELANYYNESIDRESAHEILTRIL
                     EEAHSEDHQSQMKDQRSRERREKSTLEKMLDSTVTRQVGRTVARELTRGLLGVLGVRT
                     TARRKRKSTNRGFDWF"
     gene            complement(7456..8121)
                     /locus_tag="PKM27_14405"
     CDS             complement(7456..8121)
                     /locus_tag="PKM27_14405"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR08508.1"
                     /translation="MKDPIYKMHMGKRLSLILGLLAYVCLVSGQGAIAPKIKWLTFEE
                     AEWEARQAKSPRKFMVELYTDKCGWCKKMDLSTFQNEFIAHYVNENFYPVKFNAELRK
                     DIQFNSQLFKLDKSGYHELAIALTMGDLAFPTLVFLDEDYQIIQPIQGYQTVDELERI
                     MFYFANNMHKTTPWNTFVQHYQPTNSKPRVDPRDGPDHLLIIPVPVPPHAKSKGEVIK
                     KKN"
     gene            complement(8295..9314)
                     /gene="ribD"
                     /locus_tag="PKM27_14410"
     CDS             complement(8295..9314)
                     /gene="ribD"
                     /locus_tag="PKM27_14410"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNR08509.1"
                     /translation="MDQELLMARCLQLARLGKGRTLTNPMVGALLVHEGKIIGEGFHS
                     AYGADHGEIEAFKSVTKEDQERIAYSTLYVNLEPCCYHGKTPPCADEIIRRGIKKVVV
                     GTPDPNPKVNGMGIERLKAHGVDVRFPVLEAECRALNRFFFTNMQKQRPFILLKFACS
                     KDSYIGNYHRAVPISNAWSRYIVHKVRHEVDGILIGTRTALNDNPRLTNRFYYGKSPV
                     RIVMDRYGKIPADFHLFSDGQPTWLFTESPAIRPTPVRVFAWEKELQKMMDVLMQEGI
                     GSLLVEGGAYTLQSFIDLGLWDEIWVTQSGLVLGSGVKAPKFTGNLVNEFHLRQDKLS
                     IYKPL"
     gene            complement(9318..10448)
                     /locus_tag="PKM27_14415"
     CDS             complement(9318..10448)
                     /locus_tag="PKM27_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNR08510.1"
                     /translation="MNRIYLDHAASSPMMPEVIRVVTEAMELYSGNPSSIHTEGRTAR
                     AKIEDARKKIARIFNASIGEIFFTSGGTEANNTAIKCAVRDLGVKQIISTAIEHHAVL
                     HSIEAMRREYDIRTDLLAVDAWGRFELGDLETLLQQASAPILVSLMHGNNEIGNINPI
                     RQIGELCEQYGAYFHTDAVQTAGHHGLNLQEIKAHFIAASAHKFNGPKGAGILFIRQG
                     VPVRPLLDGGGQERNMRAGTEATHQILGMAYALELAMNTLARRQAHHADIKQYFKEEI
                     LKMDAGIHINGDPEKSLDHVLSLSFPPGPKSDLLLLQLDIAGVSASGGSACTSGAEQE
                     SHVLNAIGHPPERKTIRFSFSHSNTRDEIDRVISLLRTWYSP"
     gene            complement(10445..11830)
                     /gene="glmM"
                     /locus_tag="PKM27_14420"
     CDS             complement(10445..11830)
                     /gene="glmM"
                     /locus_tag="PKM27_14420"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNR08511.1"
                     /translation="MPLIKSISGIRGTIGGTPGINLTPPDIIAFTSGYAALLKTRFPS
                     PKIVIGRDGRISGPLVQSLVASTLQAMGVHVLDAGLTTTPSLEMAVTAHHAQGGIMIT
                     ASHNSMAWNALKFMNEKGEFIDAETGQALLQVSPEQILYSPADKLGGCTVVKNSISHH
                     IKSILKLKLVDAELVKNRKFKIVVDVINSTGSIAVPALLEKLGCEFELLNGEMNGVFA
                     HDPEPLPEHLSELSNLVVKNKADLGIVVDPDVDRLAFVCENGEPFGEEYTLVAVADYV
                     TGKEKGNTVSNLSSTQALRDITLKNGGQYSASAVGEVNVVARMKETKAVIGGEGNGGV
                     IYPKLHYGRDALAGIALFLTHLAESGLPASLLRARYPNYEISKNKIELAGKSQMDLIF
                     SRLKEQYQKNKLNSEDGLRIDLDGEWVHLRQSNTEPIIRIYAESSSKVVADHLAHKLI
                     RDIREIQKELK"
     gene            11948..13093
                     /locus_tag="PKM27_14425"
     CDS             11948..13093
                     /locus_tag="PKM27_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08512.1"
                     /translation="MDKVLDKIPVTLVSLLLASLPFSIAANSVLLAALAAWMLFEMLR
                     SPDMRKNLKGAGHPIPWAVIPLIYGISILYSHDYHRALNQWLNLSPLFVMPLAFLTFR
                     LDQNISRFRVISGLALCVSIVIHILVNAGLFDLGSKEAAMGSFFHDREKNAWIFWAGF
                     LSWLGGQEHKIMNRFFQSALFLGLLYFGNLTLILLAAFCWLAAESTFTGRGFKLAGPG
                     ILIFGGSMVMFALVFFPSFSMALQEAGQNILALAGRTSVSPGLQEYFIHLKMGFLQWW
                     THPFAGTGIGDYIHAMWNEYHDHGMEAPNYPHSQWVHLLVSTGMMAFGAGWILIRSWK
                     PLTGTQRLILALVLTSFLFMAPLKSQISATALMMAVLLIPGYGRNQA"
     gene            13074..14342
                     /locus_tag="PKM27_14430"
     CDS             13074..14342
                     /locus_tag="PKM27_14430"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR08513.1"
                     /translation="MEGTRLKDSPVRVKAYIAGLILFLTGLVSSKALISLGSVILIGT
                     ACWGLFSRPSLPVRWPKQLFGFVLLFLPPFISGIWSASVQDWMQVSQNKIMLPALFAS
                     FCLAPGLEPKTMLRLGLFQAMTVVLASWYSMLTLWPDPSGISSSYLEAKTLKVFLSND
                     HLHFSLYVFITILLLLANLNAFRMQFGKGVYLTASIGLAWLVIYLHILGAKTGLLLLY
                     LTAFIYLIMNYLNGKQWLRLGAGFIVLFLLASLSISQIPTLKNRFYYTLYDFNQYIHG
                     AYIDGLTDGARVLSWKAGKAVALESPWMGKGFGDLHHTFMKWHQAHSAQLQPYNWLQP
                     SNEWLMYLCGSGIPGMLILTLGLWLIYRHSPFREHFLFLALYCSQVLMMFYEVNLTNQ
                     IGISLLVFSVGWFQCSPSPGREASFRKQGT"
     gene            complement(14355..15131)
                     /gene="mazG"
                     /locus_tag="PKM27_14435"
     CDS             complement(14355..15131)
                     /gene="mazG"
                     /locus_tag="PKM27_14435"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNR08514.1"
                     /translation="MNNDSKTAQAFLRLATIMNDLREKCPWDRKQTIHSLRNLTIEET
                     YELADAVLQNDMKALKEEIGDLMLHLLFYARLAAEQNAFTLAEALDQECDKLIHRHPH
                     VYGDLQVADDQEVKQNWEKLKLQEGKKSVLGGVPDSLPAMVKAYRMQEKTKQVGFEWE
                     HAGQVWEKVEEELSELRESLHQADDQAHIEEEFGDVLFALINYGRFIGVEAETALEKV
                     NKKFKSRFQYIEQMAGRPLSEMTLDEMNDLWNQAKSLKAS"
     gene            15176..15394
                     /locus_tag="PKM27_14440"
     CDS             15176..15394
                     /locus_tag="PKM27_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08515.1"
                     /translation="MSQFFYTLAIIFGCFFLAFFMINLRYWISGREFRGTCASAGVEL
                     RGKIGECMVCGRKPGEACGKPDKASGAV"
     gene            15571..17439
                     /locus_tag="PKM27_14445"
     CDS             15571..17439
                     /locus_tag="PKM27_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNR08516.1"
                     /translation="MCKGSCFHPVQKILGSFILTFWGIPALLLAQPEGLSDLYRKVDT
                     LIPMRDGKKLYTVIYLPRDESKRYPILMERTPYSAGPYGPDRFPSRLGPNPGLAREQY
                     IFVRQDVRGRYMSEGHNLEVTPHRPGKKTRDEVDESSDTYDTIEFLLRMIRNHNGKVG
                     IYGISYPGFYASAALPDAHPALAAVSPQAPVTDEFIGDDVNHNGAFFLMDNFNFINSF
                     GVPRSGPLMNYPGSLTDTAYSDAYRFFLELGPPGRTRDPSLFGHKSYIWNEYLEHDTY
                     DEYWRSRNIRQHLKGIRVPTLVVGGWYDAEDCFGALRTYDALEKQNSGNANTLVMGPW
                     THGAWASGNWTSFGPYAFGMNTSTFFQDSIETPFFNYYLKGIGSAPAFEAVVFETGSN
                     LWKTYEQWPPRQSKELTYYLSPRAGLGDSPAPKAKAYSSYISDPSHPVPYTAKKQRYR
                     NNLYMVEDQRFVSDRPDVLSFTTGPLEDELILCGPVEAEIYASTSGSDLDLIVKVIDA
                     HPDSGTVDGTGAQYLVRAEVFRGKFRKSYSSPSPFHKNKPEVIRFTLPDVNHCFKKGH
                     RLMIQIQSSWFPLVDRNPHQFLKIPEAGPGDYQSAEIRIYHDRKRPSSIRFNSLIP"
     gene            complement(17436..>19795)
                     /locus_tag="PKM27_14450"
     CDS             complement(17436..>19795)
                     /locus_tag="PKM27_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR08517.1"
                     /translation="FSIPQKGLSVFVYHLDKTFGDSVKTKKSESGPLYLWYGQDSLAE
                     FRDVLEYVLDKTGKSLVVKTGRPARDSLKRHTAILRHDVVHAFRDTLLAGDLDATRFN
                     WDESGNQLVFLSTRDSAAAKIKSWQVRIHQPGLDSAAVRLDPLTAVLPDGMQVSNHFT
                     PFFSEDGTRLFFGLGRIPVPEDTSVPHIDRVSLDVWHYADDYLQTMQLKNLDQEIQRS
                     YLTLSLPDGKWIPLGDERLGQVVPAAKGNGRYALGITDYGQRIPLQWEGDSKKDLYLI
                     DLETGSKKMVINDLDGQASISPAGTYLFWFDQAKSQYFSHHNPTGKTTQVNRKIRVSL
                     ADEENDEPDDPGAYGICGWSEGDQWVYLYDRFDIWKCDPSGIKTPVNITHGQGREQKI
                     QFRNVVLDPDEKYFKPDQVLLLSALQDSTKQSGFYRLALKEKSGPHPLTMGEFSYGPV
                     LKARNAERMAYTKGNFQNSPDFYTSNWFKAETRHSAINPQQANYNWGSVDLIEWTTFK
                     GKPGRGLLYKPEDFDPSGKYPMIVYFYEKLTGGRYQYIAPAPTPSRLNIPFFVSRGYL
                     VLAPDIRYEIGYPGGSAYDHVVSGVESLKKYPWLDTANIALQGQSWGGYQALHIITRT
                     TQFKAAWTGAPVVNMFSAYGGIRWESGRNRQMQYEKSQSRIGATPWQRRDLYIENSPL
                     FYIDRINTPIVIMANDADGAVPWYQGIELFTNLRRLGKKAWLLNYNGEAHNLRERKNQ
                     KDISVRQQQFFDHFLKNEPAPAWLEKGIPAVYKGKTMGYEIEIKN"
BASE COUNT         4496 a         4942 c         5150 g         5207 t
ORIGIN      
        1 ttaagacatg agggcttcca gcttgtccgt gaggaccttt ttactggttg cacctacgtg
       61 gcgatccacc agcttgccgt ttttgaaaaa cagaagagtg gggatgcttc ttacgccata
      121 ctccatggtg acttcagcat tgtcgtctac gttcagtttg ccaatggtcg ccttatcctt
      181 gtattccgcc gataattcct cgatgatcgg accgatcatc cggcagggcc cgcaccattc
      241 agcccaaaaa tctatcaatg ccactccttg tttatcaagg acttccgttt taaagttacc
      301 gtctgtaagc gtttgtgcca ttttatctta aatttaaatg aacttgtaca acaattcggt
      361 gaagcgtttg gtttattaag tccaaagatc ttgataattt taatatgatt ttaagatccc
      421 ggcacaaaat taccggaatc gttgttttct cgttcatttc ggtattggcc ttttttttat
      481 ccccgggcct gttggaaaag gtttacggtg ggtttatatt tcccctggtc agggtgatct
      541 tcagcatcct gatctggatc ccatttcctt taatatacct ggtccttttt tcgttgctgg
      601 tatggctggc ccttcttttt cgaggtaaag gcagcgttaa aaacaaggcc ttacgttccg
      661 gggcgcttac cctttcgctt attttcctgt tctattggct ctgggcttat aattataaaa
      721 ggatcccttt tggagcccgg tttccggtca ccctggcgtc ggtcgacagc caggccctcc
      781 gggaagaatt gcaattcgcg gctcagttcc tgctcgaaaa cagcccgggg catcgaagct
      841 acccttccat taaggagacc gagggcatga tccggccctg ggtcgattcg gtgtgcaggg
      901 cgtacggata ccggacaccc gggatgatcc gggtgaaagc cctttaccca aaaggtcttt
      961 tgctccggat ccgtaccgcc ggtttttatt ttccacacgg tggggaggct tatatcgatc
     1021 ccggactgca tgaactccag gtccccttta ccatggcgca tgaaatgggc catggatttg
     1081 gtgtcgcgga tgaaggggcc tgtaatttca ttgcccttat ggccctgcac cgtcacccgg
     1141 attccatgat gcgatacagc ggggccatgg gttacttcag gtatgtggcc ggcgcctacc
     1201 gcttttattt cccggaggac taccagcaat tcagggatca attgcctttg caggtacagg
     1261 atgacctgaa tcaaatcaat gcgcggttat taaagtatcc ggatatcttt ccaaaactga
     1321 gagaccgcat ttatgaccgt tatttaaaga tgcagggcat tgatgaagga ctggccagtt
     1381 atgaccggat cattgatatg gtcagggagg cgaggagaag gggagtttgg tagtggaatt
     1441 aaggaattaa ggaattaagg aattaaggaa ttaagggtgg aagagttaag ggtcaaaggc
     1501 gaaaggttaa tggtcaaggg taaggaggaa cagaccgacc attcaacaac aattcagcga
     1561 ttcaacaatt ccgcgattaa acgattccac aatctcaagc caggcaggat tctatgaggc
     1621 tggtataaaa ctcctgcagg gtatttccgg cggcacgaat ttgctggggc actatgctgt
     1681 tggcgctttg gccgggaacg gtgttgatct caagcataaa agctttcccg gatgcctcat
     1741 tgtaaataaa atcgatgcgc accacgcctt tacagttgaa gatatggtac aatttttttg
     1801 cctggttttg aatgagctcg cggatggccg ggctgaccgg ggcaggggtg atttcctcgg
     1861 ccattccggt cgtgtatttg gcttcgtaat cgaaaaattc ctttttactc cggatctcgg
     1921 tgatgggcag ggtcacgata tcctttccct gccggtacac gccaatggtc agttctgtgc
     1981 cggaaataaa ttcttcgacg atcacttcat cgtcctcttt aaacgcttta tccagggccg
     2041 gcgccagttc ttcagggctg ttgattttgc tcatgccaat gctggatccc ccattgttgg
     2101 gttttacgaa gagcggcggc tggagcggac cgaatacctg tgcgggatcc acaggatccc
     2161 ctttaaagaa atggagcgac cgggataccg ggatgccgga aaaagccgca acggctaccg
     2221 tatatctttt gttgaacgta atagcggagg aagccaggcc acaggccgta tacggcaagc
     2281 ccagcaaatc aaaatatccc tgcaattttc catcctctcc cggagtaccg tggatcccgc
     2341 acaatacggc ttcgaaagtg gttttttgcc catccagggt aaaactgaaa tcatttttgt
     2401 ccataggcac ttcaattccc tgggcagggt tgtaaaacca accgtcccgg cgtatgtcga
     2461 tccggtataa atcataccgg gcccggtcca catgggcttc aatggtcttg gccgattcgt
     2521 acgaaatcac ggcctccccg gaatatccgc cggtgacaaa agcgattttt ttcttcattg
     2581 gataaaggta cgatgggaat ttatattaag aaaaaatcta atattttaaa cggggttgtc
     2641 ctttaattta aaacaggatt tcctgcaatc gtgccagcga ttcctgcaac cggtttaaag
     2701 ctaacggaat atcccaggac tccctgttcc ttggttccac ccggttggag acggcccgga
     2761 gttgcaggca gggaacccgg tggaccatgc aggaagcaaa aaaggcggct ccttccatgg
     2821 attcaatatc gggctgcaag cgttttatca tttgatctat ttcttcctgg cggccgctta
     2881 cgcggttgac cgtgacggcc ctgacttcca gaaccccact cataatgtaa cggtccggcg
     2941 gctccagccg gtatgcctgg aagggattgt ttttttcccg tataagggta tcgagtggga
     3001 tgaaactacc atcgcggtcc tgggcaccga ggtcggcaaa ataatcttcc cggacctgca
     3061 ccacctgacc gatgccgatc tcaggacgga atgaaccggt aatgcctgcc tgtatggcca
     3121 ggcccgggat ctttttgctg aaatatgccg ccaggaagaa ggcggtcgac ggcgacccgg
     3181 ccccggtcac gagggtttca actccttccg caaagggtcc cagggcttcc agttccaggg
     3241 ctgtggctgc gcataccagg attttggtca ttaatccgtc ccgttttctt tcggaacaaa
     3301 gatatatcta agactcaaag cgggagaaat ccccagggct ggatgaaagt caaaggaaat
     3361 gtcgatggcc aggcgcggat ccggttgcag gccaataccg gcgcctaaac cagcaggatt
     3421 cgcagtaaac cccattctca gggataaaac gggcaccacc cggtagtcca gggcaaggtg
     3481 gctcttccat gggcgatcgc cggttttttc ccattctgca tacaagacaa ccccttcgga
     3541 taattggtaa gacagtccgg caagatttgc tccaggggaa gaggttttgg agccctttcc
     3601 ccgatagaga ggattataac tgctatatcc aatgcctaga tccccattga ttttgatcat
     3661 gaagcctagg tcaaaaccca aattggcggc cggttcagcc tggctgacgt gcaaaccaag
     3721 ataatgaaac gaaagtccaa tagccaggtt cggggacaat gcccgtccat aacccatacg
     3781 aaccagttgc cggaggtatt caggcagtcc gtaccattcc atgttcaatc cgaatgccga
     3841 atgggttcca gaaggaagga tggcctgaat agatccggcc cggatgccgg tccccgtgaa
     3901 ccggctttgg gcatgggctg agaaactggt ttgatgcaag tagcccagta cggatgaatt
     3961 tgacaaatac ccatcgggtt cctggaggca aatgcccaag cctcccagac cggtgctttt
     4021 tgctccgcgg atttcaggta atccgctttg ccctttaagc aaaggaataa ctgctacaac
     4081 atagaggaat atgatgcaat tagggtgcag cttgcagggt tttctcgcaa ataaagataa
     4141 ggaattcagt gatcaaggct tggggaaaaa cagaggcgca gatcaagacg acgcctgttt
     4201 tcccgcgtat atttaccggt ttaagtccgg aaaccattat ggcaaaattc aggtctcatt
     4261 atgcggggag cggtggaaaa aggggcaggg gcaaatcctt tttattccgg acggtcttgt
     4321 atggcatggt gctcctctat ttattgtatg cgcttgcccg tttcatccaa aaagcggatt
     4381 ggaaattgcc ggggaaggcg gaagaccggg ttgaatctat gggtgcactg gattcgctgg
     4441 atttttttcc agccgtcagt accgggaatc aactggtcat acaccgtcat ttcgccttgt
     4501 cctattccga aaagcatgag caggcggaat gggttgccta cgaacttacc gtggacgagt
     4561 tgaattcggc gaggcaggaa cgattcgatt atttttcccc ggaccataaa ataaccaccc
     4621 gttccgccgt acaccgggat tattccggat cgggatatac gcggggccat cttgctccgg
     4681 ctgcggatat ggcccatgat ccggtagcgg cggaagaatg ttttttcatg agcaatatca
     4741 gtccccagga gaaagctttc aacaacggga tctggaggga attggaggaa caggtgaggg
     4801 actgggcgcg gaaaaacaga agattgtatg tggttaccgg gcccatactc aatcagggga
     4861 tccgggggaa aatcggtaaa aacagggtga gtgtgccgtc attattttac aaagtactgc
     4921 ttgattacac cccgccggat tttaaaggca ttgccttcct gctccccaat gaacgcagtg
     4981 aacggaagtt gcaggaatat gcatacccca tcgacagtgt ggaagccctc accgggatcg
     5041 attttttcac taaactcgaa gcttccggag aaatggaatc cctggaaaac agcataaacc
     5101 tccaagcatg gcccttcagc caggaacggt ttaaggaaag ggtgcaagtg tggaataaac
     5161 aggaataaac cagatgcgat tccggtttat tcctgtatgg gccggtaccg gtgtaaggct
     5221 tagttggagg aaccggttac cggaggagta acgggagcgg gtgcgggtac cgctggctta
     5281 gttacttttt tagctggcgc tttttttttg gctaccggtt ttttcgcagc gggttttgcc
     5341 gcttttttag ctaccgcttt tttaggggcg gcttttttgg ctgcgggttt tgcggctttt
     5401 ttggctactg gttttttagc taccgctttt ttggccactg cttttttagc agcaggtttt
     5461 gccgcttttt ttgctaccgc ttttttagct accggttttg ccgctttttt cggttttgcc
     5521 tttgcaacag ccttagtggt ttttgaaggc ttggctgctt tggcggcctt ggcggcttgt
     5581 tcctgttttg atttttttgc aaggtcagct cggtgagacc tttcaaaagt ctggatgtct
     5641 ttaaacttac cgctggcatc tcttaccgca tataattttt taccggcaga gctgcgatgt
     5701 gtcgttcttt tactaggcat gatgatgaat tttaatgaaa caatttataa aaataactaa
     5761 tgcgaatata ttataaaaaa gattaataaa aaaaataaaa atgtttttca tttaaaaaaa
     5821 ggtccaaact tatttcttca taagtttgta aatccaaaat tcaagaatgt atgcaagacc
     5881 tttcttcccg gtttcttgaa tcgatccaaa agggttattc attcaaagga gacacgctca
     5941 tcatgggggt acccatgtat aacaaacaag tcgtaaccgg tgctttcatc cggttgcctt
     6001 tgagtaccct taaccggcac ggcctgatcg ccggggccac gggcaccgga aagaccaaat
     6061 ccctgcaggt gcttgcagaa aatctttccg cgcatggagt gccctgcctg ctgatggata
     6121 tcaagggaga tctcagcggg ctcgccatgc ccggtaccag caatgcaaaa attgaagagc
     6181 gggcggccaa gaccgggctt ccctttatac cctcggccag tccggtggaa ttgctcagcc
     6241 tttcgggtga aaaagggata cggctgcggg cgacggtctc tgaattcggg cccgtgctgt
     6301 tttcaaaaat cctggggctg aatgaaacgc aaagcagcat catggccctg gtgtttaaat
     6361 attgtgacga tcaggatctc ccgctgctcg atttgaagga catgaaaaaa gtgctttcct
     6421 ttttgtccaa tgaaggaagg gcggatatgg aaaaggaata cggccggatg tccgctgctt
     6481 cggtcggcac catcctgcgc aacatcatcg agctggagca gcagggcgcg gaaacttttt
     6541 tcggtgaacg ttctttcgat gtcaacgatc tcgtgagaag ggatgaaaag ggaaggggca
     6601 tcctgtcgat cctgcggctc accgatatcc agaacaaacc caagttgttt tccactttca
     6661 tgctgcagct cctcgccgag atttatgctt ccttcccgga agccggcgac ctggacaaac
     6721 ctaaactttg ccttttcatc gatgaggccc acctgatttt tgatgaagct tccgatgttt
     6781 tattggatca gatcgaaacc attatcaaat tgatccgttc caaaggggtg ggtgtgtatt
     6841 ttatcaccca gagccctacc gatataccgg ctgccgtgct cagccagttg ggtctgaaga
     6901 tccagcatgc tttgagggcg tttaccgctg cggaccggaa agcgattaaa gaggcagccg
     6961 agaattatcc actcaccgag ttttacgata ccgatcagtt gttgaccgaa ctgggtattg
     7021 gggaagcctt tataaccgcc ctgaacgaaa acgggattcc gacgcccctg gcccatacca
     7081 tgatgaggcc tcccgtttca agaatggata tcctggagcc cggggagatc gacaggctgg
     7141 tcagccagtc cgaactcgca aactactata acgaatccat tgaccgcgaa agcgcgcacg
     7201 aaatcctcac ccgcatcctg gaggaagccc acagcgagga ccatcaaagt caaatgaagg
     7261 atcagcgaag cagggagagg agggaaaaaa gtacgcttga gaaaatgctc gacagcacgg
     7321 tgacccggca ggttgggcgt acggtggcgc gtgaacttac ccggggactg ttaggagtat
     7381 tgggagtgag aaccaccgcc agacgaaaaa gaaaatccac caaccggggc ttcgactggt
     7441 tttaaaatat ccggatcagt ttttcttctt gatgacttcg cctttcgatt tggcgtgcgg
     7501 cggcacggga accggaatga tcagcaggtg gtcgggcccg tccctgggat ccacccgggg
     7561 ttttgagttg gtgggctgat aatgctggac aaaggtattc cagggagtcg tcttgtgcat
     7621 gttgttggca aaataaaaca tgatgcgctc cagttcgtcc acggtctggt aaccctgaat
     7681 gggctggatg atctgataat cctcatccag gaaaaccagg gtagggaagg ccaggtcacc
     7741 catggtgagg gcgatggcca gttcatggta tccgctttta tccagtttga aaagctgtga
     7801 attgaattgg atgtctttcc ggagctcggc attgaatttt accggataga aattttcatt
     7861 gacgtaatgg gcgatgaatt cattttggaa ggtggagagg tccatttttt tgcaccatcc
     7921 gcatttatcg gtatacaact ctaccatgaa tttccttgga gatttggcct gccgggcttc
     7981 ccattccgct tcttcgaagg tgagccattt tatctttgga gctatcgcgc cctggccgga
     8041 aaccaaacac acataagcaa gcagaccgag gatcaggctg agtcttttac ccatgtgcat
     8101 cttataaatt ggatctttca cggttagaga ttcaaaatta caatctaaga aacagattat
     8161 ccaatggatc tggtgcatcg atcccagttt taaaagatat ttaacgcttt ggaaggaaac
     8221 cgttgtagtt ctttggtttt ctttaggttt tatataaaaa catcttcaaa aaaaagaatt
     8281 aaagaaggtc cggatcaaag gggtttgtag atcgagagtt tgtcttggcg gaggtgaaat
     8341 tcattcacta aattgccggt gaacttaggg gctttgacgc cgcttccaag caccaggccc
     8401 gattgggtga cccagatttc atcccaaagt cccaggtcta taaaggattg cagggtgtag
     8461 gctcccccct ccaccagcaa ggacccgatt ccttcctgca tcaggacatc catcatcttt
     8521 tgaagttcct tttcccaggc aaagaccctg accggggtag gccttatcgc ggggctttcg
     8581 gtaaacagcc aggtgggttg gccatccgaa aacaaatgaa agtcggcggg tatcttgcca
     8641 tagcggtcca taacgatccg caccgggctc ttgccataat aaaaccggtt ggtcagcctg
     8701 ggattgtcat tgagggcggt tcgtgtaccg atcaggattc catcgacttc atgcctgacc
     8761 ttgtggacga tatatcttga ccaggcgttg gaaatcggga ccgcccggtg ataattgccg
     8821 atgtaggaat ccttgctgca tgcaaacttc aacaggataa aaggcctttg tttttgcatg
     8881 ttggtgaaaa aaaaacggtt caatgccctg cattcggctt cgagtaccgg aaaccggacg
     8941 tccactccat gcgctttaag cctttctatg cccatgccat tgacttttgg attgggatca
     9001 ggcgtaccca cgaccacttt ttttataccc cggcgaatga tttcgtctgc gcagggcggg
     9061 gttttaccat gataacaaca gggctccagg tttacataca gcgtggagta ggcgatgcgt
     9121 tcctgatctt cttttgtcac cgatttaaaa gcttcgatct caccgtggtc agccccgtaa
     9181 gccgaatgaa aaccttcccc gatgatcttc ccctcatgga ccagcaatgc gccaaccatg
     9241 ggattggtga gggtccggcc tttgcccagc ctggccagtt gaaggcagcg tgccatcagg
     9301 agttcctgat ccatctgtta agggctgtac caggtacgga gcagggaaat cacccggtcg
     9361 atctcgtccc tggtattgga atgtgagaag gagaaacgga tggttttccg ttccggagga
     9421 tggccgatgg cgttgaggac atgggattcc tgctcggcgc ccgaggtaca ggcgctccct
     9481 cctgaagcgc tcacgccggc gatgtccagt tgaagcaaca acaaatctga ttttggcccg
     9541 ggagggaaag aaaggctgag cacatggtcc agcgattttt ccggatctcc gtttatatga
     9601 atgccggcat ccattttgag gatctcttcc ttaaaatatt gtttgatgtc cgcatgatga
     9661 gcctgccgcc gggccagggt gttcatagcc agttccaaag cataagccat gcccaggatc
     9721 tggtgcgtgg cctcggtccc cgcgcgcatg ttccgctcct ggccgcctcc gtccagcagg
     9781 ggacggacag gcactccttg ccggataaag aggatgccgg ctccttttgg cccgttgaat
     9841 ttatgagcac tggccgcgat aaaatgggcc ttgatttcct gcaggtttaa accatggtgc
     9901 cctgccgtct gaacggcatc ggtgtgaaaa taagcgccat attgttcgca gagttctccg
     9961 atctgccgta tgggattgat attcccgatt tcattatttc catgcatgag gctgaccagg
    10021 atgggagcgg aagcctgttg cagcagggtt tccaggtcgc cgagttcaaa acgaccccag
    10081 gcatcgacgg caagcaggtc ggtgcggata tcgtattcgc gcctcatggc ctcgatgctg
    10141 tgcaatacgg cgtgatgttc aatcgcggtg gaaatgattt gttttacccc gaggtcgcgc
    10201 acggcgcatt tgatggccgt attgttggct tccgtacctc ctgaagtaaa aaaaatttca
    10261 ccgatggatg cattgaatat cctggcgatt tttttgcggg catcttcgat tttggctctc
    10321 gcggtcctgc cttctgtatg aatggatgag ggattgccgc tgtacaactc catggcttcg
    10381 gtaaccaccc ggatgacttc aggcatcatg ggcgaagaag cggcatgatc caggtagatc
    10441 cggttcattt gagttctttt tgaatttccc ggatgtcccg gatcagttta tgggcgaggt
    10501 ggtcggccac cactttactg gaactctccg cgtatatccg gatgatgggt tcggtgttgg
    10561 actggcgcag atgcacccac tctccgtcaa gatcgatgcg gaggccatct tcactgttga
    10621 gtttgttttt ctggtactgt tctttaagcc gggaaaaaat gaggtccatc tggctttttc
    10681 cggccagttc aatcttgttt ttggaaatct cataattcgg atacctggcc cgcaggaggc
    10741 tggccggcag gccggattcc gccaaatggg tcaggaagag ggcaataccg gccagggcat
    10801 cccttccata atgcagcttg gggtaaatga ccccgccatt cccttcaccc ccgatgacgg
    10861 ccttggtctc cttcatcctg gccactacat tgacttcccc tacggccgat gcggaatatt
    10921 ggccgccgtt tttgagcgtg atgtccctga gggcctgggt tgaagaaaga ttggacaccg
    10981 tattgccttt ttccttgccg gtgacgtagt ctgcaacggc caccagcgta tattcttcgc
    11041 cgaagggttc cccgttttca cagacaaaag ccaggcggtc cacatcaggg tccaccacga
    11101 tgcccaggtc cgccttgttt tttaccacga ggtttgacaa ttcggaaagg tgttcgggaa
    11161 ggggttccgg gtcgtgggcg aaaacgccat tcatctcccc gttgagcaat tcgaactcgc
    11221 atcccaattt ttcaaggagg gccggaacgg cgatgcttcc cgtgctgttg atgacatcca
    11281 ccactatttt gaatttccgg ttcttcacca attcggcatc caccagttta agtttgagga
    11341 tggatttgat atggtggctt atactgtttt tcaccacggt gcatccgcca agtttgtccg
    11401 cgggactgta taatatttgt tccggagaga cctggagcaa ggcctggccg gtttcggcat
    11461 caataaattc tcctttttcg ttcatgaatt tgagggcgtt ccaggccatg gaattatgac
    11521 tggcggtgat catgatgccg ccctgggcat gatgggccgt caccgccatc tcaagcgacg
    11581 gggtagtggt caggccggca tcgaggacat ggacgcccat ggcctggagg gtggaggcca
    11641 ccagggactg gaccaaaggg cctgaaatac ggccgtccct gccgatgact atttttggac
    11701 tggggaaccg cgttttcagc agtgcagcat atcctgaagt aaaagcgatg atgtccggag
    11761 gggtcaggtt gattcccggt gtgccgccga tggtgcccct gataccggag atggatttga
    11821 ttaatggcaa ttaaagtggt ttatgcacaa aggtagaaga ttattaaaaa tgccctaatt
    11881 gattgagtat aaatattttc aacggtatag ccaggattgc aagccttata tttggccact
    11941 gtctcctatg gataaggttt tggataagat cccggtgacc ctggtcagtt tattgctggc
    12001 cagcctccca ttttcaatag ctgccaattc ggttcttttg gccgccctgg ctgcctggat
    12061 gttatttgaa atgctacgtt ccccggacat gagaaaaaac ctcaaagggg caggacaccc
    12121 gatcccctgg gcagtgattc ccctgattta cgggatcagc atcctttatt cacacgacta
    12181 tcaccgggcc ttaaaccagt ggctgaacct gtcgcccttg tttgtaatgc cactggcctt
    12241 tttgactttc cgcctggatc aaaatatttc acgtttccgg gtgatatccg gtttggccct
    12301 ttgcgtttcc attgtgattc atatactggt caatgcaggc ctgttcgacc tcggctccaa
    12361 agaagcagct atgggcagtt ttttccatga ccgcgaaaaa aatgcctgga tcttctgggc
    12421 cggtttcctt tcctggctcg gcggacaaga acacaaaatc atgaaccggt ttttccaatc
    12481 cgcccttttt ctcggtttgt tgtatttcgg caatctgacc ctgatcctgc tcgcggcttt
    12541 ctgctggctt gccgcagagt caaccttcac gggcaggggc tttaagcttg cgggcccggg
    12601 aatcctgata ttcggtggga gcatggtaat gtttgccctg gtgttttttc cctctttttc
    12661 catggccctg caggaggcgg gtcaaaatat attggccctt gccgggcgga cttcggtaag
    12721 cccgggatta caagaatatt ttatacacct gaaaatgggt tttcttcaat ggtggaccca
    12781 tccttttgca gggacaggga ttggagatta tattcacgcc atgtggaatg agtaccatga
    12841 ccatggcatg gaagccccca actacccgca ttcgcaatgg gtccatctgc tcgtaagcac
    12901 cggcatgatg gcttttgggg caggctggat ccttatccgg agctggaaac ccctgacggg
    12961 gacgcagagg ctaatcctcg ccctcgtgct gaccagtttc cttttcatgg cccctttaaa
    13021 atcccagatc agtgctaccg cactgatgat ggccgtatta ttgattcccg gatatggaag
    13081 gaaccaggct taaggattcg ccggtccggg tgaaggcata tatagcaggc ctgatccttt
    13141 ttttgacggg cctggtaagc tccaaagccc tcatcagcct gggatccgtg atcctgatcg
    13201 ggactgcctg ctggggcctg ttttccaggc cgtctcttcc ggtgaggtgg ccaaaacaat
    13261 tatttggctt tgttttactc ttcctgcctc cttttatttc gggcatctgg tctgcatccg
    13321 tccaggactg gatgcaggta agccaaaata agatcatgtt gcccgccttg tttgcctcct
    13381 tctgcctggc cccgggtctt gagccgaaga ccatgctcag gcttggcctt tttcaggcca
    13441 tgacggtagt ccttgcctca tggtattcca tgctgaccct ttggccggat ccttcaggga
    13501 taagttcaag ttatctggaa gctaaaacgc ttaaagtttt cctgtccaac gatcatttgc
    13561 atttcagcct ttatgtattt attaccatcc ttctcctgct ggccaacctg aatgcgttca
    13621 ggatgcagtt tggaaaaggt gtttacctga ccgcatccat tggcctggcc tggttagtta
    13681 tttaccttca tatcctggga gccaaaacag gtcttctatt gctttacctg accgcattta
    13741 tctatttgat aatgaattat ctgaatggga aacaatggct caggcttggg gctggcttca
    13801 tcgtcctctt cctcttggcc tccttatcca tcagccaaat tccaaccctt aaaaacaggt
    13861 tctattatac cctctatgat ttcaaccagt atatccacgg ggcctatatc gacggcttga
    13921 cggatggggc cagggtcctg tcctggaaag cgggtaaagc cgtagccctg gaaagcccct
    13981 ggatgggaaa gggattcggc gatcttcacc atacttttat gaaatggcat caggcacata
    14041 gcgcccagct ccagccttac aactggcttc agccttccaa cgaatggctg atgtatttat
    14101 gcgggtccgg catcccggga atgcttatcc tcacccttgg cctctggctg atataccgtc
    14161 acagcccttt ccgggagcat tttttgttcc ttgccttgta ttgcagtcag gtgttgatga
    14221 tgttttatga agtgaatctt acgaaccaga tcgggatcag tttactggtc ttttccgtag
    14281 gctggtttca gtgttcaccc tctcctggcc gtgaagcgag cttcaggaag caagggacct
    14341 gatgggatgg gtgatcagct tgccttcagg gatttggcct ggttccagag gtcgttcatt
    14401 tcatccaggg tcatttccga aagcggcctg ccggccattt gttcaatgta ctgaaacctg
    14461 gatttaaatt ttttattgac tttttcaagg gcggtttccg cctctacgcc gataaaacgg
    14521 ccataattga tgagggcgaa aagcacatcc ccgaattcct cttcgatatg ggcctggtcg
    14581 tcagcctggt ggagactttc ccggagttcc gagagctctt cctccacttt ttcccaaacc
    14641 tgaccggcat gctcccattc aaaacccacc tgtttggtct tttcctgcat ccggtaagcc
    14701 ttcaccatgg caggcaggga gtccggcacc ccgccaagta cggacttttt gccttcctgc
    14761 agttttagtt tttcccagtt ttgtttcact tcctgatcgt cggctacctg gaggtcccca
    14821 tacacatggg ggtgccggtg gatcagtttg tcgcattcct ggtccagggc ttctgcaagg
    14881 gtaaaggcgt tttgttcggc cgcaaggcgg gcataaaaaa gcaggtggag catcaggtcc
    14941 ccgatttctt ccttaagggc cttcatgtcg ttttgaagga cggcgtcggc cagttcgtaa
    15001 gtctcttcga tcgtcaggtt gcggaggctg tgaatggttt gtttcctgtc ccaggggcat
    15061 ttttcacgca ggtcattcat gatggtggcc agccggagaa aagcctgggc ggttttggaa
    15121 tcgttgttca tgcctggaaa aaacggtatc tttaaggctt aaatatacac ggattatgtc
    15181 ccaatttttt tataccctgg ccattatctt cggatgtttc ttcctggctt ttttcatgat
    15241 caacctgaga tactggatca gtggccgtga attcaggggg acctgcgcca gcgctggggt
    15301 ggaactccgg ggtaaaatcg gagaatgcat ggtctgcgga aggaaacccg gggaggcctg
    15361 cggaaagccg gataaggcaa gcggcgcagt ttaaactttg cggcccgaca ttttggagta
    15421 tgcgtacaag cagaaagaca agagggtgat caccgcaacg acgtacagga tttgcatatc
    15481 aggataataa atttggttgg ttataaactg actcaaatat cctaaacgga aaatatatat
    15541 cgcataattt cttatatatt tttttttgtt gtgtgtaagg ggtcttgctt tcatccggtt
    15601 caaaagattt taggttcgtt tatacttact ttttggggca ttcccgccct gcttctggcc
    15661 caaccggaag gcctttctga tctgtaccgg aaggtggaca ccctcatccc catgcgggac
    15721 gggaagaaat tgtacaccgt gatctatctg cccagggacg agtcaaaaag atatcccatc
    15781 ctgatggagc gcacccctta ttctgccgga ccttatggcc ctgaccggtt tccttcccgc
    15841 ctcgggccca atcctggcct ggcccgggaa cagtacatct tcgtcaggca ggatgtgcgg
    15901 gggagatata tgagcgaagg ccacaacctg gaagtgaccc ctcaccggcc cgggaaaaaa
    15961 accagggacg aagtggatga aagttcggat acctacgaca ccatagaatt cctgctccgg
    16021 atgatccgca atcacaatgg caaagtgggt atttatggca tctcttatcc gggcttttac
    16081 gcgtcggccg ctttaccgga cgcccacccg gccctggccg cggtcagccc gcaggccccg
    16141 gtcaccgatg aattcatcgg tgacgatgtc aaccacaacg gcgctttttt cctgatggat
    16201 aattttaatt ttatcaattc cttcggagtg ccccggtccg gccccctcat gaattatccc
    16261 gggagcctga cggataccgc ttattccgac gcttaccggt ttttcctcga attgggacct
    16321 ccgggcagga cccgggaccc ttcgctgttt ggacataaaa gctatatctg gaatgaatac
    16381 cttgaacacg acacctatga cgaatactgg cgatccagga atatccgcca gcatctcaag
    16441 gggatccgcg tgcccaccct ggtagttggc ggttggtatg atgcggagga ctgttttggc
    16501 gccctgcgta cctacgacgc ccttgaaaaa cagaattcgg gcaatgccaa caccctggtg
    16561 atgggcccct ggacccatgg cgcctgggct tccggcaact ggacctcttt tggcccctac
    16621 gcctttggca tgaataccag cacttttttt caggacagca tagaaacccc tttctttaat
    16681 tattacctca aaggaatagg gtccgcaccc gcctttgaag ccgttgtttt cgaaaccggg
    16741 tcgaatcttt ggaagactta cgaacaatgg ccccctcgac aaagcaagga gttgacctat
    16801 tacctgtcac cccgtgcggg cctgggggac tccccggcgc ccaaagccaa agcctattcc
    16861 tcctatatca gcgatccaag ccacccggtg ccttacaccg cgaaaaaaca gaggtaccgg
    16921 aacaatcttt atatggtcga agaccaaagg tttgtctctg acaggccgga tgtgctgtct
    16981 tttacgaccg gtcctttgga ggatgaactc attctatgcg gcccggtgga ggccgaaata
    17041 tatgcctcca cctcaggttc ggacctggac ctgatcgtaa aagtcatcga tgcgcacccg
    17101 gattccggaa ccgtggacgg aacaggtgcc cagtatttag tcagggcgga agtattcagg
    17161 ggaaaattca ggaaaagcta cagttcaccc agccctttcc ataaaaacaa acccgaggtg
    17221 atccgtttca ccctgcccga tgtcaaccat tgttttaaaa aaggtcaccg cctgatgatc
    17281 cagatccaga gcagttggtt ccccctcgtg gaccgcaacc cacaccagtt cctgaagatt
    17341 ccggaagccg ggccgggaga ttaccagtca gccgaaatcc ggatctatca tgaccgtaaa
    17401 aggccctcca gtatccgctt taattcttta attccttaat ttttaatttc aatttcatat
    17461 cccatcgtct tccccttgta aaccgcaggg ataccctttt ccagccaggc aggagccggt
    17521 tcgtttttaa ggaaatgatc aaaaaactgt tgttgccgga ccgaaatatc cttttgattt
    17581 tttctttccc ggaggttgtg ggcttccccg ttgtaattga gcaaccaggc ttttttcccc
    17641 agacgacgta ggttggtgaa cagttctatg ccctggtacc agggaactgc cccatcagcg
    17701 tcgtttgcca tgattacgat aggagtgttg atccggtcaa tataaaacaa gggcgaattt
    17761 tcaatgtaga gatcgcgtcg ctgccagggc gtagctccga tccggctttg cgatttttca
    17821 tattgcatct gtcggttgcg ccccgattcc cagcggatcc ctccgtaggc gctgaacata
    17881 ttgacgacag gggccccggt ccaggccgcc ttaaactgag tggtccgggt gatgatatgc
    17941 agggcctggt agcctcccca ggactgtccc tgcagggcga tgttggcggt atccagccag
    18001 ggatattttt tgagggattc cacgcctgaa accacatggt cgtaagcgct tccgccggga
    18061 tagccgatct cataccggat atccggggcg aggaccaggt aaccccggct cacaaagaag
    18121 gggatgttga gcctggacgg ggtaggcgca ggggcaatgt actggtatct tccaccggtc
    18181 agtttttcat aaaagtaaac gatcatcggg tattttccgg agggatcaaa gtcttccggt
    18241 ttatagagga ggccccttcc gggcttgccc ttgaaggtgg tccactcgat caggtccaca
    18301 ctgccccagt tgtaattcgc ttgttgagga ttgatggcgc tgtgtctggt ctcggcttta
    18361 aaccaattgc tggtgtagaa atccgggctg ttttgaaaat tccctttggt atatgccatc
    18421 ctctccgcat tcctggcttt taatacgggt ccataactaa attcgcccat ggtgagtggg
    18481 tggggacctg atttttcttt taaggccagg cggtagaacc cgctttgttt ggtggaatcc
    18541 tgcaaagcgg ataaaagcaa tacctggtcc ggtttaaaat atttttcatc cgggtcgagc
    18601 accacatttc taaactggat tttttgttca cggccctgtc catgggtaat atttacgggt
    18661 gttttaatac cggacgggtc gcatttccag atatcaaacc ggtcatagag gtaaacccac
    18721 tggtctcctt ccgaccatcc gcagatgcca taggcgcccg ggtcgtccgg ttcatcgttt
    18781 tcttcgtccg ccaggctgac acgtatcttc cggttgacct gggtggtttt gcccgtggga
    18841 ttgtgatggc tgaaatactg gcttttcgcc tggtcaaacc agaacaggta agtccctgcg
    18901 gggctgatag aggcctgccc gtccaggtcg ttgatcacca ttttttttga tcctgtttcc
    18961 aggtctatga ggtaaaggtc ctttttgcta tcgccttccc attgtaaagg aatgcgttga
    19021 ccataatccg taatcccgag ggcgtaacgg ccattccctt tagcggccgg cacgacttgt
    19081 ccaagacgtt catcgcccag aggaatccat ttcccgtccg gaagggagag ggtaagatag
    19141 gacctctgga tttcctggtc caggtttttc aactgcatgg tttggaggta atcgtccgca
    19201 tagtgccaga catccaggct gacccggtcg atgtggggta ccgacgtatc ttcaggaaca
    19261 ggaatcctgc ccagtccaaa aaatagcctg gttccgtctt cactgaaaaa aggggtaaaa
    19321 tggttgctca cctgcatgcc atccggcaat accgcagtca agggatccag tctgaccgcg
    19381 gcgctgtcca atccaggttg gtgaatccgg acttgccagc ttttaatttt agcggcagcg
    19441 ctgtcccggg tcgataaaaa aacaagctgg tttccggact cgtcccagtt aaagcgggtg
    19501 gcgtccaggt cgccggccag cagggtatcc cggaatgcat gcaccacatc atggcgcagg
    19561 atggccgtat gccttttgag gctgtccctt gccggcctgc ccgttttcac caccaggctt
    19621 ttaccggttt tatcaaggac gtattccaat acatcccgga attccgcgag agagtcctgc
    19681 ccgtaccaca aataaagagg gccgctttcg gatttctttg tttttaccga atccccgaag
    19741 gtcttgtcca ggtgataaac gaaaaccgaa agcccttttt gaggtatcga aaatg
//
LOCUS       DAOQXT010000090        19575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3148_length_19575_cov_7.386066, whole genome shotgun sequence.
ACCESSION   DAOQXT010000090 DAOQXT010000000
VERSION     DAOQXT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19575
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3148_length_19575_cov_7.386066"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..12557)
                     /locus_tag="PKM27_14455"
     CDS             complement(<1..12557)
                     /locus_tag="PKM27_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNR08518.1"
                     /translation="MNFFSKRIQGRIPTPLYKTVKHKLKFLIRTFSFLASGLVLAYSG
                     WKWYDQNQPLALMAGGPSGSVFRDYNGDGVKQSGEPLVPGVTVNAYDASGALCATTVS
                     NFSNTAPNYTLPMGCSGQIRIEFVIGSLDDCLDPGIDFTALNGAAYGSSVQFVDASTM
                     TPVNFGLYNPADYNRGATNSKVFIPCYTTGDPLVVGTSRSGAWFLGYPYNNSGTVAKS
                     NMVTGETIGSTWGVAYSDQAKVVFSAAFLKRHVGLGTLGSGGIYKLVPTPTSFTATSF
                     YDMDANGHRTRAAAGCVAYGNGSSFTINAAPGTEVTYNGVVDPLTGLACGLGVIGTNA
                     QRGLLGDKTAKSHDPAAFDQVGKVGLGDMEISDDGLFLFVTNLYSRRIFRLELNDAYN
                     PTAIVAVDSFNLPSPSPSVTNGVLRPFATKYHRGKLFVGALSTGENGGVNTVGGATDM
                     NAYVFELDNANTAPSLTAMSQTLTYPLNYLKGPSRRDMTGVNRGEKWNPWTRNNDVSL
                     STNAANEYRTYPTPMLTDIEFNERGDLIMAYTDRSGHQWGWINDRHFTGTVLIRYIVG
                     GDILIAGVNCNTGAYTLESNGSFNSTSIGFTTNTVVSAPNVNGVGNMDGPGGGEFFDQ
                     EFYNPYHDETAQGSMAVLPGDNKIITTIMDPLGIFEGGTIKFSTLNGDESHRYRLFEN
                     ATGNFGKANGLGDLELDGLIPPLEIGNRVWFDTDMDGVQDPGEAPIANVTVELYDGVT
                     LIGTDVTDVNGNYYFDTLNVADGNQSIGGNQKGPQPNRTYTIRIGSADWTGGAGTGDL
                     TGYVLTMDNVGGAGQPDVRDNDGVLSSMIPTISYTTGAPGQNDHTLDFGFILADEPIV
                     DLALRKTIDTILQDGTNPTALNNGDTVKFKIQLFNQGELGMDSLNVTDYLPAGFKFVN
                     ATGTGQVNEGWDGTDPNNPVYKWRSNTGSGGGGSLTTAQTLPLTAQTGDGVNSLSFMH
                     NPGTGSNRLLVLGIAVGNDDGTGEPPTVTTAQFQSVNLDFVGTVQSPGAGGSNENDVR
                     IYIYKQNNPTGSGNIDISISGTGDISAQAVTFNGVDLSNPLGAFQNTSGLTSPVSNMF
                     SASSGQVVFSMLGVDGGTSITSTGGQTVIFNGSNDQVSGAAVYENGPNPTTTFTLGGD
                     QDWALGAVPINVYSQPPFNPGDTLEICIYTTLEHVPGSPTAADFTNFAEISFARDTNG
                     VDQSANDIDSPLNNNPNDNAGGQQGSPADDYINGDGSGTPGDGVAPTDQDNHDPAFLN
                     IFDLALKKTIDTGSISGNGLYTIGTDITFNITVTNQGTDTAQNIEITDYLPCGLSFSL
                     APPNPGWTRTGSLATYTAPGPLAPGQSLIVPIILQISPSDSCNPDPLDYINVAEISGA
                     EDDEGNTYTSDVDSDYDQIKNNDAGGNVNSPSDDVITGDGTGTPNDEVADTDEDDQDP
                     AELLVFDLALRKIVSPATPGYPGPYNINDLIRFDITIFNQGNIDADTVSIVDNLPAGL
                     SFTAGDNTGLNWVAGPGANQVQRTLNMVGGDTLNAGEDTTVSIFLRVLHTSGGIGAYT
                     NISEITSATAIIDGLNVTMSGDADSPYDANANNDAGGQVTSPADNEILGNGTGSPGDG
                     IASGDEDDQDPALVTIVDVALKKLISASTPGPYQYGDNVQFEITVTNQGTIALDSMEV
                     TDYIPSGFTFGANPDWTLSGSDAMRVIRSRLDPSEDTLITINLVIAAESDLSKVDSAW
                     INYAEVSQAFDTLGTDVSLLDIDSDLDNNPGNNAGGRPLSDADNYIDGDGTGVIGDGV
                     DTTDQDNHDPALVSVVDVALRKIAADTLGDASLAFGDTIRFDIQIFNQGNVVIDSLTI
                     TDYIPEGFAYLPLFNPGWSGSYPSPQYGWGTSDDLLPGESDTISIYLQIVSVGEPNLA
                     KYTNYAEISFASDTLGVNQSSNDSDSPLNTLDDDNAGGQPLSPADDYIDGNGSGAIGD
                     GVDSTDQDNHDPASVYPRVVDVALVKRIGFTTPGPFTLGDTVVFDLKIVNQGTITLDS
                     VYLVDYIPAGFAFDAVLNPVWTLLGNQASTIVDETLLPGQSTIVSISLIILKITDLSL
                     VDSAWTNRAEITRIFDEDGNDVSSEDADSPLDNDPDNNPGGQPGSSADDEENGDGTGS
                     PGDGVPGTDQDNADPAFVDLPDLALRKIIQDTLGDGSLNYGDTIKFGIQLFNQGNIPV
                     DSIRIEDSIPSGYSFVPGSLNAGWILAGQDASYDWGQGDTLFPGEIDTICIYLTLNMV
                     TPSAAGHWINRAEITYFADTTGTPLVDIDSNPDNEQTNDPGGSPDTPSDDAIDGDGTG
                     MPGDTSRTTDEDDEDPAGLKFYDLALVKQVAAGSTVTAYGDSVKFLLTIINQGTEPVD
                     SILITDTLPAGLMFDNSLPVNNIWTPVGNELTTMLRTRLDPGEDTVICLALKVLKNYD
                     LSVAGSTWVNRAEISSAFDTLGMDIDDRDIDSPLDENYGNNGGGQPESPADDETGGNG
                     TGPVGGPDSGTDQDNADPALVEVADLALRKVAMDTLGDNVLLFGDTVKIAIQIFNQGS
                     IPVDSIVITDSLPAGLGFVTGPLNAGWVFNGSDYEYGWGTGDTLYPGESDTVCIYVIF
                     QLGFPGTPETYTNLAEISEFFDTDGNPLDDVDSAPDDNQANDGGGEPKTGSDDPIDGD
                     GTGMPGDTSSVTDEDDEDPLELGFFDLALRKVVNAGSTATAYGDTVKFDLMIFNQGSI
                     PTDSIILTDSLPAGFSFDATIPGNAGWVQSGNKLTKAVSGLLLTGLEDTTVCLWLTVE
                     QVLNPADHATAWINYAEISEAWLAGNNISILDIDSELNNIYGDNAGGQPDSPADDEVD
                     GDGTGAIGDGVDTTDQDNADPARVEIVDLALRKIIMDTLGDATLEYGDTIKFAIHLFN
                     QGNVPIDSLVVNDTLPNGFSYVNDPGLNAGWVFAGTEANYGWGTGGTLFPGESDTICL
                     YLRLEMVTSGLPDFTNRAEISYAQDTTGADRSNDDIDSPLNDNYGDNAGGQPGSPADN
                     EVDGVGTGMIGAGVDTTDQDNADPAFVEVVDLALRKIIVDTLGDATLEYGDTVKFAIH
                     LFNQGNVAIDSLMVNDTLPSGFSFVNDPGLNAGWTNPLGNETSYFWGESDTLNPGESD
                     TICIYLRLEQVNTGVPDFTNRAEISYAEDLTGADRSNDDIDSPLNDQYWDNAGGQPES
                     PADNEVDGDGTGALGDGVAPTDQDNADPALVQVVDLALRKVILDTLGDATLEYGDTIK
                     FAIHLFNQGNVPVEELTVTDTLPNGFSFVDNSLNTGWSLPAGNETQFVWGAGDTLNPG
                     ESDTLCIYLRLEMILSGIPDFVNRAEISSATGFGETDLSDSDIDSPLNGNYGDNAGGQ
                     PDSPADDEVEGIGTGAIGDGVDSTDQDNADPARVEVLDLALRKLLVPGPRMTVVYADT
                     AEFIIEVINQGNVPATNIKITDSLPDGFVADAGVNPGWTTVNSTSAYSTYCYTLTDTL
                     HPGDTASLNFYGIVESSEMQGAWINYAEITSVQDTTGADRSGDDIDSSPDGNVGGNDG
                     GGQPESEADDETNGDGTGAIGDGVASTDEDDEDPAALMLFNLALKKEIDGSTPGPYRY
                     GDTVRFVVTVVNQGNIPATNIEVSDSIPAGFAFDAGLNPDWTGPGPVVTLVFTDTIAM
                     GDTTTALLDLIVQPVVDPMLRQNAWTNLAEISYAEDTSGNNTDTNLPDHDSYPDDDFT
                     NDAGGRPESPADNYLNGNGQGQPGDGVAETDEDDADPALVEVFDLALVKQIDSAGTPL
                     PYTYGDLIKYKLRVINQGNEPAVNIEVADYIPQGLVFDGPSNPEWSLIDDSTAVTTIA
                     DTLFADEDTVLCIYLRLQPAADRNAWVNVAEISYAEDSTGADRSNDDADHVLDRDRTN
                     NAGSQVNSPADDYVDGDGTGSAGDGIPGTDGDNIDPAWLDVFDLALIKTTPHLVVQEG
                     EVVDFYITVYNQGNIDAENIKVTDYIPLDMSLAASPGWTVLPPDSACYTIPGPLFVGD
                     SITIQINLQVDDPAVNLPEGLYNYAEISSATDTMDADYPDIDGDFDADPGNDPGGDLF
                     NDDDNNISGNGKRGEDEDNHDGAYVMVCKGYVCNDRVNVSFGDLCEIIVTGDDVIENP
                     MLPSIHYRVTLRDASGKIVPDARLTRAHMGYCFTYSVDAPACPANSCWGTICVEDKAP
                     PMIDCSADTVLCFELSNLPSEGMV"
     gene            13143..14381
                     /locus_tag="PKM27_14460"
     CDS             13143..14381
                     /locus_tag="PKM27_14460"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNR08519.1"
                     /translation="MYLLTKDRVYLGYLIYILTIIAYFLGTAITEPFPSLFKCAFCFD
                     GFMKLCMGLAYFNFLYSVVFVPDSIPNAVRKWMHRLFRVTILLTLFSYPFIWLGPRFL
                     TDLTFGLLGLLGILSLSSLVAIMRKNIPLYIHLIILGSLLLVGSFVVTEFLVQTNTRF
                     RTNPNLLFHTFNSIATILEILLFMVGLLLRFGDKLKENYSLQQRLLTIENEALKSREA
                     MLRSKINPHFVQNIFNILSLNLPAGEAFDFLRKYIYQAGTFFRKTLTVTENTVHSLEE
                     EIEYIEEYLKMQQLLLKNYFSYSIRIDPDIDTYSMKVPTMILQPLVENSLKHGLSGLD
                     YPGEIIIQVTEENGHPAILVEDNGRGLEENQVIRQGHGLSLTQNRLAALKTNGIPATF
                     KIVKNPERKGITTKIEFISV"
     gene            14378..15154
                     /locus_tag="PKM27_14465"
     CDS             14378..15154
                     /locus_tag="PKM27_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR08520.1"
                     /translation="MKKNLCIYHIDDEYVYLDQVRQIVEQTPGLIYLGGENNAEKGLK
                     AVQNLRPDILLLDIEMPGKNGLYVADQLKGSNTFIVFLTNYTDYSIAAFNAFALHYLL
                     KPLSQMALLEVIERYKYITKTQAPSFAQKEQLQELKGYFQKQLKYPTRIFINTQKEII
                     VLPLNEIVFLSAEGSYTSFSLKDGRALISGKNLKTYASVVEQHPDFVRVHRSYIINQA
                     YLDKIEKKKPGLIFHFTSGTILSMNSFRKEEWFDKLNLVQ"
     gene            complement(16310..>19575)
                     /locus_tag="PKM27_14470"
     CDS             complement(16310..>19575)
                     /locus_tag="PKM27_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08521.1"
                     /translation="FAVARMADIEKARADYLDKIEAECGLKEYKEHKVWYDRYIEDWI
                     NMYVFDDELHMDDCGENQVVLRVYEADSMPRLDPHVWKCGAHEWFCYNSYPSYRLVHN
                     YHFFSTNPPASKDCDGGAPWLCKKASEQWFADRKNQFEFSRYYGTNDGANFPQTRLYN
                     DCMVKVIVDDKQPPVVDHLEDLTVYCDHAPAGKAYARQLCKQGEEFEYWPGPLSYQGE
                     TYGYYGGSDHIRRGHTDHQDPVYGCDHGDQWTPVYCRKWLYLDSFDQSGPVDPAQYFS
                     TLVKFDKDRPARPLGAKEFSITDNCRLDDTTMHITDIGSVNGCGEGWYQRTWTIKDKC
                     GNVATAIQKVIVKHRSDFEVVFPADLEVTCDTENATDSSHTGKPVITDDECEVIGVRY
                     VDEISTIDQGDGACYKIIRTWTLIDWCIYDPNAHTIHPDVIVDDRLRANDTDRVCVFR
                     NLKDNGDGFMKYVQIIKVFDHEAPVLNCRDTTICLTEGCLQGVNIPLEGADNCSEEVL
                     YRLDITRPDGSIETRVNVNSISGNFTAGVYTIRIIGKDHCENADTCTMRLTMRDCKAP
                     TPYCLNGIATVVMPLTGAIEIWAKDLDRGSTDNCTPNSALKFSFSSNVGEASRVFRCS
                     DIPDGKQQAIPVDIWVTDQAGNQDYCRTYILLQDNGGQAGGVCKDTTLTTANINGKLY
                     TEDKEGVEFATVELKGVGQAAIPSFKTGSDGNFAFSNIQLTGPVSIKALRDDNPMNGL
                     STLDLVLLQKHLLGTEKLKSPYKMIAADINNSQDISVVDLVELRKLILGLYDQIPGNT
                     SWKFIPKSYSFTDVQNPWGYPTEEQLDKMQETEVRDFVGVKIGDLNSSAVPHSLIGTE
                     VRGNDIGLTLETEDRVVRKGEEVSVTFRSPNFRGISGWQGTLHTGGLQYLGVRQAGKL
                     DLTEEHIGRRWAGEGMLTMSWHHHTGLDLDEETPLFTLVFRAGVDGRLSEMLRFGSQH
                     TIAESYEGKGELGGLSLHFVAADGNAVASRSELYQNYPNPFDQRTVIGINLAKEGKGV
                     LRILDITGRTLRSFEREWSKGYQEVWLDRREIGATGVLHYRFESEFFTASRKMMIVD"
BASE COUNT         4531 a         5278 c         4774 g         4992 t
ORIGIN      
        1 accatccctt ccgaaggcag gttcgacaat tcaaaacaca agaccgtgtc cgcgctgcag
       61 tcgatcatcg gcggcgcctt gtcttccacg caaattgttc cccaacagct gttggccggg
      121 catgccggtg catcaacgga ataggtgaaa caataaccca tatgagcccg ggtgagcctg
      181 gcatccggca ctattttgcc ggaagcatcc cgaagggtta cccggtaatg aatagagggc
      241 aacatgggat tttcaatgac atcgtccccg gtcactataa tctcgcaaag atctccaaaa
      301 gagacattca cccggtcgtt gcaaacatat cctttgcata ccatgacata ggccccgtcg
      361 tgattgtctt cgtcttctcc ccgcttgccg ttccctgata tattattgtc atcatcgttg
      421 aacaggtctc ccccgggatc atttccggga tccgcatcga aatctccgtc gatatccgga
      481 taatccgcat ccatcgtatc ggttgcggaa gatatctcgg catagttgta aagcccttcc
      541 ggcagattta ccgcaggatc atccacctgc agatttatct ggatcgtgat gctgtcccct
      601 acgaacaaag gacctggaat ggtgtaacag gcgctgtcgg gtggcaggac agtccatccc
      661 ggactggctg ccaggctcat atcaagcggg atataatcgg tcactttgat attctccgca
      721 tcgatgttac cctggttata gaccgtgatg tagaagtcca ccacttcgcc ttcctgcacc
      781 accaggtgcg gggtcgtctt gatcaaggca aggtcgaaca catccagcca ggccggatca
      841 atattatccc catcggtacc tggaattcca tcaccggcgc tacccgtacc gtctccatcc
      901 acatagtcat ccgcagggct gttgacctgg cttcctgcat tattggtccg gtcacggtcc
      961 agaacatggt cggcatcgtc gttgctgcga tcagcacccg tggaatcttc ggcataactg
     1021 atctcggcca cattgaccca ggcattccgg tcggcagcgg gctgcaacct caggtaaatg
     1081 cacaataccg tatcttcatc tgcgaagagg gtatccgcaa tcgtggtcac cgcagtgctg
     1141 tcatcgatca ggctccactc cggattggag ggcccgtcga agaccaatcc ctggggaata
     1201 taatccgcga cttcaatatt gaccgccggt tcatttccct gattgatcac ccgcagcttg
     1261 tatttgatga ggtctccata ggtataaggc aatggagtgc ccgcagaatc aatctgttta
     1321 accaaagcca ggtcgaaaac ctccacgagg gccggatccg catcatcttc atcggtttcc
     1381 gccaccccgt ctccaggctg accctgcccg tttccattca ggtaattatc tgccggactt
     1441 tccgggcgcc ccccggcatc gttggtgaaa tcatcatccg ggtaagaatc atgatccggc
     1501 aggttggtat cggtattgtt gccggaagtg tcttccgcat agctgatctc ggccaggttg
     1561 gtccaggcat tctgccggag catggggtca acgaccggtt ggacaataag gtccagcagg
     1621 gccgttgtgg tgtcgcccat cgcaatcgtg tccgtaaaca ctaaggtcac cacggggcct
     1681 ggtccggtcc aatccggatt caatccagca tcgaaagcaa aaccggctgg tatcgaatca
     1741 ctgacttcaa tattggtcgc cggaatattg ccttggttga ccacagttac tacaaagcga
     1801 accgtgtctc cataccggta aggtccggga gtggaaccat ctatttcctt tttcagggcc
     1861 aggttaaaca gcatcagggc tgcagggtct tcgtcgtcct cgtcggttga agctacacca
     1921 tcccctattg caccggtacc atctccgttg gtttcatcat cggcttcgct ttccggctga
     1981 ccgcccccat cgtttccacc tacatttcca tccggagagg aatcaatatc gtctccactg
     2041 cggtcggctc cggtcgtatc ctgaactgag gtaatctctg cataattgat ccaggcacct
     2101 tgcatctcag atgattcaac aatgccataa aaattcaggc tggcggtatc ccccggatga
     2161 agggtatcgg tcaaggtgta acaatacgtg ctgtacgcag aggtggagtt taccgtggtc
     2221 catcctgggt ttacgccggc atctgccaca aatccatccg gcagcgaatc cgtaatcttt
     2281 atattggttg ccggtacgtt tccttgattg atcacttcaa taatgaattc cgccgtgtcc
     2341 gcataaacca ccgtcatccg cgggccgggt accagcaatt tcctcaaagc cagatccaag
     2401 acctccaccc gggcgggatc ggcattgtcc tgatccgtgg agtccactcc atccccaatg
     2461 gctcccgtgc ctataccttc cacttcatcg tcggccgggc tgtccggctg accgccggcg
     2521 ttatctccat agttgccgtt caaggggctg tcaatatcgg agtcgctcag gtcggtttcc
     2581 ccaaaacccg ttgcactgct gatctcggct cggttcacaa aatccggtat cccactcagg
     2641 atcatctcca acctcaggta aatgcaaagg gtatcgctct cgcccggatt cagggtatcc
     2701 cctgctcccc agacaaattg ggtttcattt cctgcaggca aagaccaacc ggtattcagg
     2761 ctgttgtcaa cgaatgaaaa tccgttaggt agggtatccg tgacggtcag ttcctcgacc
     2821 ggcacattgc cctggttgaa caaatgaatt gcaaatttga tcgtatcacc gtattcgagg
     2881 gttgcatcac ccagggtatc caggatcacc tttcttaaag caagatccac cacctgtacc
     2941 aaagccgggt cagcattgtc ctgatcagta ggagcaactc catcacctaa agcgccggtg
     3001 ccgtccccat ctacttcatt gtcggcagga ctttccggtt ggccgccggc attgtcccag
     3061 tattgatcgt tcaacggact atcgatatca tcgttgctcc ggtccgcacc ggtaagatct
     3121 tcagcatagc tgatttcagc ccggttggtg aaatcgggca cacccgtatt cacttgttcc
     3181 agtcgcaggt aaatgcaaat ggtatcgctt tcgcccggat tcagggtatc gctttcgccc
     3241 cagaaataac tggtttcatt accgagtgga ttggtccacc ctgcattcag gccaggatca
     3301 ttcacaaaac tgaatccgct gggcagggtg tcgttgacca tcaggctatc gatggccaca
     3361 ttgccctggt taaataaatg gatcgcgaac ttgaccgtat cgccatactc cagggtcgcg
     3421 tcgccgagtg tatccacgat gatcttacgc aacgccaggt ctacaacctc gacaaaggcc
     3481 ggatccgcat tgtcctggtc agtggtatcc accccggctc cgatcattcc ggttccaact
     3541 ccatccactt cattgtcggc cggacttcca ggctgacctc ccgcattatc tccgtaatta
     3601 tcattcagcg gactgtcaat atcatcgttg ctccggtccg ctccggtcgt atcttgtgca
     3661 taactgatct ccgcccggtt ggtgaagtca ggtagaccgg aggtcaccat ctccaggcgc
     3721 agataaaggc agatcgtatc gctttcgccc ggaaacaggg tgccgccggt gccccatccg
     3781 tagttggcct cggtccccgc aaatacccat cctgcgttca gtccaggatc atttacataa
     3841 ctaaatccgt tgggcagggt gtcgttgacc accagactgt caatgggcac attgccctgg
     3901 ttaaacaagt ggatcgcgaa tttgatggta tcgccgtatt ccagtgttgc gtcgcccaaa
     3961 gtatccatga tgatcttacg cagggccaga tccacaatct caactcttgc cggatcggca
     4021 ttgtcctgat cggtggtatc cactccgtca ccgatcgccc cggttccatc cccgtccacc
     4081 tcgtcgtccg cgggactgtc cggttgtccg ccggcattgt ctccgtaaat attattcaat
     4141 tcagaatcaa tgtccagaat gcttatatta ttgcccgcaa gccatgcttc actgatttct
     4201 gcataattga tccaggccgt cgcatgatcc gcaggattca ggacctgttc gacggtaagc
     4261 cataggcaaa ccgtagtatc ttccagcccg gtcagcagga gtccgctcac ggctttagtc
     4321 agcttgttgc ccgactgaac ccagcctgca tttccaggaa tcgttgcatc gaaactgaaa
     4381 cccgccggca gggaatccgt gagaatgatg ctatctgtgg ggatgcttcc ctggttgaat
     4441 atcattaaat caaacttcac cgtatctcca taggcagtgg cggtacttcc cgcgttgacc
     4501 actttacgga gcgcgaggtc aaagaagccc agttccaatg ggtcttcatc gtcctcatcg
     4561 gtcacactgc ttgtatcacc gggcataccg gttccgtctc catcgatcgg atcatcagac
     4621 ccggtcttgg gttcaccgcc tccgtcattg gcctgattgt cgtccggagc agagtccaca
     4681 tcgtcgagcg gattgccatc ggtatcaaaa aactcactga tttccgccag gttggtatag
     4741 gtctctgggg tacccggaaa tcccaactga aaaatcacat agatacagac ggtatcgctt
     4801 tcaccgggat aaagggtgtc cccagttccc cagccgtatt cgtaatcgga cccgttaaat
     4861 acccagcccg cattcagcgg accagtcaca aatcccaaac ccgccggaag ggaatcggta
     4921 atgacaatac tgtctaccgg aatactaccc tggttaaaga tctggatggc gatttttact
     4981 gtgtcgccaa acagcaggac attgtcgcca agggtgtcca tagccacttt tcgcaaagcc
     5041 aggtcggcca cctctaccag tgccgggtcg gcattatcct ggtcggtgcc ggaatccgga
     5101 ccgccaaccg ggcccgttcc attacctccc gtttcgtcat ccgcagggct ttcgggctgc
     5161 ccaccgccat tgttgccata attttcatcc aaaggggaat caatatcccg gtcgtcgata
     5221 tccataccca gggtatcaaa tgccgaactg atttcagccc gattgaccca ggttgaccct
     5281 gcgacgctta aatcataatt tttaaggact ttcagcgcaa ggcagattac cgtatcctct
     5341 cccgggtcca gccttgttcg cagcatggtg gtcaattcat taccaacagg agtccagata
     5401 ttatttacag gaagtgaatt gtcaaacatc agacccgctg gtaaagtatc ggtaatgagg
     5461 atgctgtcca cgggctccgt accctggttg atgatcgtca gcaagaattt gactgaatcc
     5521 ccgtacgcag ttaccgtact gcctgcagca acttgtttta ccagtgccag atcgtaaaat
     5581 ttcaaaccgg cggggtcttc gtcgtcctca tcggtggtcc ggctggtatc tccgggcatg
     5641 ccggttccat ccccatcgat ggcatcatcc gacggagtgt ctggggaacc tccgggatca
     5701 ttggtctgct cattatccgg attggaatca atgtccacca gaggggtccc tgtggtatcg
     5761 gcaaaataag tgatttcagc cctgtttatc cagtggccgg ccgcagaagg cgtcaccata
     5821 ttcaaggtaa ggtaaatgca aattgtatct atttcacctg ggaaaagcgt atctccctga
     5881 ccccagtcat agcttgcatc ctgtccggcc aagatccagc ctgcattgag gctaccgggc
     5941 acaaaactgt agccggaagg tatggaatcc tctatccgga tactgtctac cgggatattg
     6001 ccctggttga ataactggat cccaaatttt attgtatctc cgtagttcag agagccatcg
     6061 cccagcgtat cctggatgat tttacgcaga gccagatcgg gaaggtccac aaaagcagga
     6121 tccgcattgt cctggtccgt gcctggcact ccatcaccag gactgccggt cccgtcgcca
     6181 ttttcttcgt cgtcggccga actgccaggt tgtcctccgg ggttattgtc cggatcattg
     6241 tccaggggac tgtctgcatc ttcacttgaa acatcattgc cgtcctcatc aaagatccgg
     6301 gtgatttcag cccggttggt ccaggcgctg tctaccaggc tcaggtccgt aatctttaaa
     6361 atgatcaggg atatgcttac aatcgtggat tggcccggta aaagggtttc gtccacaatc
     6421 gttgatgcct gattaccgag cagagtccag accggattta aaacggcgtc aaaggcaaaa
     6481 cctgctggga tatagtctac gagataaaca ctgtcgaggg taatggtgcc ctggttgacg
     6541 attttcagat caaatacgac cgtatctcca agggtgaagg gcccaggggt ggtaaagcca
     6601 attcttttaa ccagggctac atccacgacc cgcggataaa ccgaggccgg gtcgtgattg
     6661 tcctgatcgg tggagtctac tccgtctcct atggctccgg atccgttacc atcgatataa
     6721 tcatcggcag ggctgagcgg ttggccgcct gcattgtcgt catccagggt gttcagcgga
     6781 ctgtccgaat cattcgaact ttgatttaca cccagggtat cggaggcaaa actgatctcc
     6841 gcatagttgg tatattttgc cagattgggt tcaccgacgg atacaatctg taagtatatg
     6901 ctaatagtat cgctctcacc gggtaagaga tcatccgaag tgccccatcc gtattgcgga
     6961 cttggataag atccgctcca gccagggttg aataggggca ggtaggcgaa gccctccgga
     7021 atataatccg tgatggtcaa gctgtcgatc accacattgc cctgattaaa gatttgaatg
     7081 tcgaaccgga tggtgtctcc gaatgccagg ctggcatcac ccagcgtatc cgcggcgatc
     7141 ttgcgcaaag cgacatccac tacgctgacc aatgccggat catggttgtc ctggtcggtt
     7201 gtatccactc cgtctcctat gacgcccgtc ccatcgccat cgatgtagtt gtcggcatcg
     7261 ctgagggggc ggccgcctgc attattgccg ggattgttgt cgagatcact gtcaatatcc
     7321 agcaggctga catcggtacc cagggtatcg aaagcctggc tgacttctgc atagttgatc
     7381 caggcactgt ccactttaga caggtctgat tcggctgcga tgaccaggtt aatcgtaatc
     7441 aaggtgtctt ccgaaggatc cagacgggac ctgatgaccc gcattgcatc ggatccagac
     7501 aaggtccaat ccggattagc accaaaagta aaaccggagg gtatataatc cgtcacctcc
     7561 atgctgtcca atgcaatggt tccctgattg gttacggtga tttcaaactg tacgttgtct
     7621 ccatattggt atggcccggg tgtggaggcc gaaatcagtt ttttcaaagc cacgtcgact
     7681 atggttacca gggcaggatc ctggtcgtct tcatcgccgg aagcaattcc atcgccgggt
     7741 gatcccgtgc cgttgcctaa gatctcgttg tctgccggtg aagtcacctg ccccccggcg
     7801 tcgttgttcg cattggcatc atagggagaa tcggcatccc cgctcatggt cacgttcagg
     7861 ccatcgatga tggcagtggc ggaagtgatt tccgaaatat tcgtgtaggc cccaatcccc
     7921 ccggaggtat gaaggaccct caggaagatg gacaccgtag tgtcttcccc ggcattcagg
     7981 gtatccccgc caaccatatt gagggttcgc tggacctggt tggcgcccgg tcctgcgacc
     8041 cagttcagtc cggtattgtc tccggctgtg aaactcagac cggctggaag attatccacg
     8101 atgcttacgg tatccgcatc gatattgcct tggttgaaga tcgtgatatc aaaccggata
     8161 aggtcgttga tgttgtatgg ccccgggtag cctggggtag ccggtgaaac gatctttcgc
     8221 aaggccaggt caaatacaag cagttcggcc ggatcctgat cgtcctcgtc cgtatccgcc
     8281 acttcgtcat tgggcgtgcc ggtgccgtcg ccggtgatca catcgtcgct gggggagttg
     8341 acattgcccc ctgcatcatt gtttttgatc tggtcgtaat cgctgtccac atctgaggtg
     8401 taggtgtttc cttcatcatc ctcggctccg ctgatttcgg cgacgttgat ataatccagg
     8461 ggatcaggat tgcatgaatc cgaaggactg atttgaagga tgatgggtac gatcagggat
     8521 tggccgggag ccagtggccc tggagcggta taagtagcca gtgaaccggt cctggtccat
     8581 cccgggttgg gtggcgccag ggagaaacta agtccgcagg gtaaatagtc cgtgatttct
     8641 atattctgag cagtatcggt accctggttg gtcaccgtga tattgaatgt aatgtcggtg
     8701 cctatggtgt agaggccatt acccgatata ctgcccgtat caatggtttt tttcagggcc
     8761 agatcgaaaa tattcaagaa tgccgggtcg tgattgtcct ggtcggtggg cgctaccccg
     8821 tcgccgggag ttccggaccc gtctccattg atataatcat cggccgggct gccctgttgc
     8881 cctcccgcat tgtcattggg attgttgttc aatgggctgt cgatgtcgtt tgcactttgg
     8941 tccacgccgt tggtatcccg ggcaaagctg atttcggcaa aattggtaaa atcagccgcg
     9001 gtgggactgc ccggcacatg ttccagggtg gtgtaaatac atatttcgag ggtatcgccc
     9061 gggttgaagg ggggttggct gtagacgttt attggtacag cgcctaaagc ccaatcctga
     9121 tctccaccca aggtaaaggt ggttgttgga ttgggaccat tttcatatac cgctgctccg
     9181 gatacctgat cgttcgatcc attgaaaatg acagtttggc caccggtact tgtaatggac
     9241 gttccgccat cgactcctaa catgctgaaa acgacctgcc ctgaagaggc cgagaacatg
     9301 ttgctcaccg gacttgtcaa tccggaggtg ttttgaaatg ccccgagtgg attggagaga
     9361 tctaccccgt taaaggtgac cgcttgggcg gaaatatctc cagtgcccga aatgctgata
     9421 tcgatattcc ccgacccggt tggattgttt tgtttgtata tataaattct gacgtcattt
     9481 tcatttgaac cacccgctcc cgggctttgt actgtgccca caaaatccaa attaacggat
     9541 tgaaattgtg cagtggtcac cgtgggcggc tctcctgtgc catcgtcatt tccaacggca
     9601 atacccagga ccaataacct gttggatccg gtaccgggat tatgcatgaa ggacaaggag
     9661 tttaccccat ctccggtttg ggcagtcaaa ggcagggtct gagcagtagt gaggcttcct
     9721 cctccgccgc ttcccgtatt gcttctccat ttataaacgg gattattggg gtctgttccg
     9781 tcccaacctt cattgacctg gccggtgccg gtcgcattga caaacttgaa tccggcgggt
     9841 aaataatccg tgacattcag gctgtccatc cccaattctc cctggttgaa gagctggatt
     9901 ttaaatttga ccgtatcgcc attattcagg gcggtgggat tggtgccatc ctgcagtatc
     9961 gtgtcgatgg ttttgcgtaa ggccaggtcg acgatgggct cgtcggccag gataaatccg
    10021 aaatcaaggg tgtggtcgtt ctgtcccggg gctcctgtag tatagctgat ggtggggatc
    10081 atcgaagaca atactccgtc attgtccctt acatcgggtt ggcccgcccc ccccacattg
    10141 tccatggtga gtacatatcc ggtcaggtcc ccagtccctg ccccgcctgt ccagtctgcg
    10201 gagccgatcc ggatggtata ggtccggttg ggctgcggac ctttctggtt gccccctata
    10261 gattggtttc catcggccac gttcagggtg tcgaaataat aattcccgtt gacatcggtg
    10321 acgtccgtgc cgatcagggt cacgccatcg tataattcca cggtgacatt ggcgatcgga
    10381 gcttctccgg gatcctggac tccatccata tcggtatcaa accagacgcg gttgccaatc
    10441 tccaggggag ggattaaacc atccaattcg aggtcgccga gtccgttggc tttcccaaaa
    10501 ttgccggtgg cattttcaaa cagacggtac cggtggcttt catctccgtt gagggttgag
    10561 aattttatgg ttcctccttc aaaaataccc aggggatcca tgatggtggt gatgatctta
    10621 ttatctccgg gaagcactgc catggaacct tgggcggtct catcgtgata aggattatag
    10681 aattcctggt caaaaaactc gcctccgcca ggaccatcca tattacctac cccgttgaca
    10741 ttgggtgcgg atacgacggt gttggtggta aaacctatac tcgttgagtt aaaggatccg
    10801 ttgctttcca gggtgtaggc gccggtattg cagttaacgc cggcaatcaa gatatctccg
    10861 cccacgatgt accggatcag cactgttccc gtaaagtgtc tgtcgttgat ccatccccat
    10921 tggtgtccgg aacggtccgt ataggccatg atcaggtccc cgcgctcgtt gaattcgata
    10981 tcggtgagca tgggggtggg ataggtgcgg tactcgtttg cagcgttggt gctcagggat
    11041 acatcattgt tccgggtcca gggattccac ttttccccac ggttaacccc ggtcatatcc
    11101 ctccgtgaag gtcctttgag ataattaagc gggtaggtca gggtctggct catagcggtg
    11161 agggaaggag cggtgttggc attgtccagt tcaaatacat aggcattcat gtccgtggct
    11221 ccacctaccg tattgacacc accgttttcg ccggtgctga gggcgcccac aaataatttc
    11281 ccgcgatggt atttggtggc aaagggcctg agcacgccgt tggtgaccga agggcttgga
    11341 gaaggcaggt taaaactgtc gactgcgact atggccgtcg gattgtaagc atcgttaagt
    11401 tcaaggcgaa aaatccggcg ggagtataag ttggttacaa aaaggaaaag cccgtcatca
    11461 ctaatttcca tgtcaccaag gccaaccttt ccgacctggt caaaagcagc cgggtcatgg
    11521 cttttagctg ttttatcccc taaaagtcca cgctgggcat ttgttcctat taccccaaga
    11581 ccacaggcca aaccggtcag aggatccacc accccattat aggtgacttc cgtgcctggc
    11641 gcggcattta tggtaaagct gcttccattg ccataagcga cgcatccggc ggcagcacgt
    11701 gtccggtgtc cgttggcatc catatcataa aaagaagtgg cggtaaagga agtgggagta
    11761 ggcaccagtt tataaattcc accgctcccc aatgttccca gtcccacatg tcttttgagg
    11821 aaagcggcgg aaaaaactac cttggcctgg tcgctgtagg ccacacccca ggtggaacca
    11881 attgtttccc cggtgaccat attggatttg gcaaccgtac cggaattgtt atagggatat
    11941 ccgaggaacc aggccccgga acgggaggtt ccaaccacga gcggatcacc ggtggtataa
    12001 caaggaatga agactttaga attggtggcc cctcggttat aatcggccgg gttgtacaag
    12061 ccgaaattga ccggggtcat ggtggaggcg tccacaaact ggacggagga tccgtaggcg
    12121 gccccgttca gggcggtgaa atcaattccc ggatccaggc agtcatccag ggaaccgatt
    12181 acgaattcga tccggatctg accgctgcaa cccatgggca aggtgtaatt gggagccgta
    12241 ttggagaaat tactgactgt ggtggcgcac agggccccac tggcgtcata ggcgttgacc
    12301 gtcacgcccg gtacaagcgg ctccccgctt tgtttcactc catccccgtt gtaatcgcgg
    12361 aatactgaac cggaggggcc gccggccatc agcgcaagcg gctgattttg atcgtaccat
    12421 ttccacccgc tataggcgag gaccagcccg gaagccagga aggagaaagt acgaatcagg
    12481 aatttgagtt tatgtttgac cgttttataa aggggggtag gaattctccc ctgaatccgt
    12541 ttggaaaaaa aattcatgaa atgacagtat ttgtaatcct cccttcatca aagtccgggc
    12601 cgaccacaaa tccatccctt ttttatctgt accggaaaat cattcatagg gtagctcccc
    12661 agcgaccaga agctttaatc cagaccatca aacgttaaat tcatattgct ttccaattaa
    12721 ttcctaatag actaattatc aggttattaa acttgatttt ttttataata ctctgtttac
    12781 tgtttttgga aagaggtttt aattcaaacg caatcctctc ggggacagga ctaatgccat
    12841 gaaataatta atacagaaag atcacggacc gaaaaaggcc ttcacaaagt atttaggcta
    12901 ttttgcaaat ccggtcaaac aactattaaa aaaaaacatt cccgctgcgc aagaaaggag
    12961 gaagggtaaa agaaatttgc ctggtttagg gtattttaaa tattaagtaa tagttttata
    13021 taaattttta tttttgtaca agtagttgaa cctatttcca atcctcgtgt gtgtcccttg
    13081 agcaagtgat ttcgataatc ctgtttactg taacaggggt tcaattggtt tttatttctg
    13141 gtttgtattt actgaccaag gacagggtct acctgggtta cctgatttat atcctgacca
    13201 taatcgccta ttttttaggc accgccatca ccgagccgtt cccttccctg tttaaatgtg
    13261 ccttttgttt tgacgggttt atgaagctct gcatgggcct ggcttatttt aattttcttt
    13321 attcggttgt tttcgtcccc gactcgattc cgaatgcagt gagaaaatgg atgcacagat
    13381 tgttccgcgt cacgatttta ctgacgttat ttagttatcc tttcatatgg ctggggcctc
    13441 gttttttaac cgacctgacc tttgggctat tgggcctgct cggcatcctg agtttaagca
    13501 gcctggtggc gatcatgcgt aaaaatattc cactatatat tcacctgatc atcctgggct
    13561 ctctcttgct ggtggggtct tttgtcgtaa ctgaattcct ggtccagacc aacacccgct
    13621 ttcgaacaaa cccgaatttg ctttttcaca ccttcaactc cattgcgacc attctggaaa
    13681 ttctcttatt tatggtcggc cttttattgc gttttggaga caaactgaag gagaattact
    13741 ctttgcaaca aaggctgctc accatcgaaa atgaggccct aaaatcacgg gaggccatgt
    13801 taagaagtaa aataaatcca cattttgttc aaaatatttt taatatactt tcattaaacc
    13861 tcccggctgg ggaggcgttt gattttctga ggaaatacat ctaccaggca ggaacctttt
    13921 tcagaaaaac cttgaccgtt accgaaaata cggttcattc cctcgaagag gaaattgaat
    13981 atattgaaga gtatttgaaa atgcagcaac tcctgttgaa gaactacttt tcctattcca
    14041 tccggataga tccggatatc gacacctact ccatgaaagt gcccaccatg attttacagc
    14101 ccttggtgga aaacagtctc aagcatgggc tttccggact ggactatccc ggcgaaatca
    14161 taattcaggt tacggaagaa aacggacacc cggcgatttt agtggaagac aacggcaggg
    14221 gcctggaaga aaaccaagta atccggcaag gtcatggctt atccctcact caaaataggc
    14281 tggcagcact gaaaaccaat ggaatacccg ccactttcaa aatcgtgaaa aatcctgaaa
    14341 gaaaaggaat caccacaaaa atcgaattta tatccgtatg aaaaaaaacc tgtgcatata
    14401 tcatattgat gatgaatatg tttacctgga tcaagtcagg cagattgtcg aacagacgcc
    14461 cggattgatt tacctcggcg gagaaaacaa tgcggaaaag ggtctaaagg ccgttcaaaa
    14521 cctgcggccg gacatcctcc tgctggatat cgagatgccg ggtaaaaacg ggttgtatgt
    14581 tgcggaccaa ctcaaagggt caaatacttt cattgtattt ctgaccaatt atactgatta
    14641 cagcatcgcc gcttttaacg ccttcgccct gcattatctt ctaaagcctt tgtcccagat
    14701 ggccctcctc gaagtcatcg aacgttataa atacatcact aagactcagg ctccatcctt
    14761 tgctcaaaaa gagcaattgc aggaactaaa agggtacttc cagaaacaac tgaaataccc
    14821 gacccggatt tttatcaata cgcaaaaaga aattatagtc ctcccgctga atgaaatcgt
    14881 atttttatcg gccgaagggt cctatacttc cttcagcctt aaggatggca gggcattgat
    14941 cagcgggaaa aatttaaaga cctatgcatc cgtggttgaa caacaccccg attttgtacg
    15001 ggtacaccgg agttatatca tcaaccaggc ctaccttgac aaaattgaaa aaaagaaacc
    15061 cggtttaatc tttcatttta cctccggtac catcctgagc atgaactcct tccggaagga
    15121 agaatggttc gataaactaa acctagtcca ataggttaaa tccttatttt tttaaccagg
    15181 gaagtgcctg aaaagtctat tctcactaca tagaggcccg gaggtaaatt tggaagagtt
    15241 atgagattgg atctcacctg ctcgtggaaa acgatttgac catgggaatt aaagagcatc
    15301 aacaagccgg cttccaattt cagctcatcc ccagtcaaac gaatttgtct ttctgttcca
    15361 acgtatcgga tgctcgcagc aagggaggaa ggtcgaagat accggactgg ggaataagat
    15421 acctcgccac tcagcgtcac ggatttcagc ctgaaataag ggtcctggcc atattggttt
    15481 aaacagaagg acgccatatt ttcctggagc ttaaaatctc cagctcttaa ggtggataat
    15541 aaataaaaag caatgccatc cttgctgcac tccaacgaaa attccgtacc ggcttcgagt
    15601 gatgccatgc tccagctgaa acagatgcgg ttgggtagag ccccttggac cctgaaattg
    15661 gacagatcca aagccaattg tccggaagcc acattccctc cactggaaga gaagatatcc
    15721 ccacacgcat tgcctccgca agaaggattg gactgaccga ccattcttac ataaaccacc
    15781 tccccacagg ggcaggaaaa tgcggaagaa gtgaagattt gtgtggaagg attattccaa
    15841 cgaaaacaat ccggtaacaa tatttcctga ccttgtgatc cacaagtata gaatgttttt
    15901 actgaggcat tcccatggtc cagagttatg gaagcttgaa tgatataggt acatgaaccg
    15961 gttaaactat cggtacggat gacctcagca ctattgacct caatagccga acattgagga
    16021 taaaccaaat aacaacacgg gccccaaagg accaaggaga tcagcagctt cttcataatc
    16081 ttcagtataa agggaatttt tctttatacc ttattcaaat gtaaccttgt tgaggggcaa
    16141 accatacgaa aggttcatcc ttcaatatgc attgattttt aaacctgaca ttctgatgaa
    16201 tagaaccaga tttttattca tgaacaagta gatatatttt attttaatac ataaaaaaag
    16261 ccccggatca ttccggggct tcccctttgt attaaagcat acgaagccct caatctacaa
    16321 tcatcatctt gcggcttgcg gtgaagaact cggattcaaa gcggtaatgt aacactcccg
    16381 tagcccctat ttccctgcgg tccagccata cttcctggta tcctttcgac cattcgcgtt
    16441 cgaaggaccg gagggtccgg cccgtgatgt ccaggatccg gagcacccct ttcccttcct
    16501 tggccaggtt gatgccgatc accgttcgct ggtcaaaagg attgggatag ttttgataca
    16561 gttcactccg ggacgccacc gcattgccgt cggccgccac aaagtgcagc gacagaccac
    16621 ccagttcccc cttgccttca taactttctg ctatggtgtg ctgggaccca aagcgaagca
    16681 tctccgacaa tcgcccatcc acaccggctc tgaacaccag tgtaaacagc ggggtctctt
    16741 catccaggtc cagacccgta tgatggtgcc agctcatggt aagcatgcct tcaccggccc
    16801 agcgacggcc gatatgttct tccgtcagat ccagttttcc agcttgtcgg acgcccagat
    16861 actgcaggcc tccggtatgc agggtacctt gccagccgct gatcccccgg aagtttggcg
    16921 accggaaggt cacactcact tcctctcctt ttcggactac ccggtcttcg gtttccaggg
    16981 tgaggccaat atcattgccc ctcacctccg taccgatcag gctgtgcggt accgcgctgc
    17041 tgttcaggtc cccgatcttt actcctacaa aatcccgaac ttccgtctcc tgcatcttgt
    17101 ccaactgttc ttctgtggga tatccccagg gattctgaac atccgtaaag gaatacgatt
    17161 tcggtataaa tttccagctc gtgttgcccg gtatctggtc gtagagcccc aggatcagct
    17221 tgcgcaattc gaccaggtcc accacactga tatcctggct gttgtttata tctgccgcga
    17281 tcattttata cgggcttttc agcttttccg taccgagcag gtgtttttgc aataaaacca
    17341 ggtccagggt gctcaatccg ttcatcggat tgtcatccct cagggccttg atgctcaccg
    17401 gaccggtcaa ctggatattg ctgaacgcaa agttgccgtc agaacccgtc ttgaaggatg
    17461 ggatcgctgc ctggcccact cctttgagtt cgactgtcgc gaattcaacc ccttccttat
    17521 cctccgtata aagtttacca ttgatgttcg cggtggtcag tgtcgtatcc ttgcatactc
    17581 cgcccgcctg gcctccgttg tcctgcaaca aaatatacgt gcggcaatag tcctggttcc
    17641 cggcctgatc ggtcacccag atatccaccg gtatcgcctg ctgcttccca tccgggatat
    17701 cgctgcagcg gaatacccgg ctggcttcgc cgacattgct gctgaagctg aacttcaagg
    17761 ccgaattcgg ggtgcaattg tccgtacttc cccggtccag gtctttggcc caaatttcta
    17821 tggctcccgt caggggcatt accacggtcg caatcccgtt caggcaatag ggcgtgggtg
    17881 ccttgcagtc ccgcattgtc agcctcatcg tacacgtatc cgcattttcg caatggtctt
    17941 taccaatgat ccggatcgta tagaccccgg ctgtaaaatt gccgctgatc gagtttacat
    18001 tcacccgggt ctctatcgaa ccatccggcc gggtgatgtc gagccggtac agaacctctt
    18061 ccgaacagtt gtccgcacct tccagcggga tgttcacccc ttgtaggcaa ccttccgtga
    18121 ggcagatggt cgtatcccgg cagttcagca ccggcgcctc atggtcaaag accttgatga
    18181 tctggacata tttcataaaa ccgtctccat tgtctttcag gttgcggaac acgcagaccc
    18241 ggtccgtatc gttggcccgc aggcggtcat ctacaatcac atccggatgg atcgtatgcg
    18301 cattcgggtc gtaaatgcac cagtcgatca gggtccaggt ccggatgatc ttatagcagg
    18361 ctccgtcgcc ctgatctatc gtcgagatct catccacata cctcaccccg atgacctcgc
    18421 attcatcatc cgtgatcacc ggtttgccgg tatgactgga atccgtcgca ttttccgtgt
    18481 cgcaggtcac ctccaggtca gcaggaaaga ccacctcgaa atcgctgcgg tgtttcacaa
    18541 tcactttctg gatggccgtc gccacattcc cacatttgtc ttttatggtc caggttcgct
    18601 gataccatcc ttccccgcac ccgttcaccg agccgatatc cgtaatatgc atggtcgtat
    18661 catccaacct gcagttgtcc gtgatcgaga attcttttgc tcccaaggga cgcgcaggcc
    18721 ggtccttatc aaatttgacc agggtgctga aatactgggc cggatccacc ggaccgctct
    18781 gatcaaaact gtccaggtac agccacttgc ggcaatacac cggcgtccat tggtctccgt
    18841 ggtcacaacc gtacaccggg tcctggtggt ccgtatgccc tcggcggata tgatcgcttc
    18901 cgccgtaata tccgtaggtc tcgccctgat aggacagcgg gcccggccag tactcgaact
    18961 cttccccttg tttgcataat tgccgcgcat aggccttccc ggcaggggca tggtcgcagt
    19021 ataccgtgag gtcttccagg tgatccacca ccggcggttg tttgtcatcc acgatcactt
    19081 tcaccataca atcgttgtac agtctcgtct gggggaaatt cgccccgtcg tttgttccgt
    19141 aatacctcga gaattcaaat tgattcttcc ggtccgcaaa ccattgctca ctggccttct
    19201 tgcacaacca gggcgccccc ccatcacaat ccttgctggc cggagggttg gtcgaaaaga
    19261 aatggtaatt gtgcaccagg cggtaggaag gataggaatt gtagcagaac cattcgtgcg
    19321 ctccgcactt ccatacgtgg ggatccaacc gcggcatgct gtccgcttcg tacaccctca
    19381 ggaccacctg gttttccccg cagtcgtcca tatgcagttc atcatcgaat acatacatgt
    19441 tgatccagtc ttcgatatac cggtcgtacc agactttatg ctccttgtat tccttgagcc
    19501 cgcattccgc ttcgatttta tccaggtaat ccgccctggc tttttcgata tctgccatcc
    19561 gcgccaccgc aaaat
//
LOCUS       DAOQXT010000091        19506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3163_length_19506_cov_6.501465, whole genome shotgun sequence.
ACCESSION   DAOQXT010000091 DAOQXT010000000
VERSION     DAOQXT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19506
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3163_length_19506_cov_6.501465"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PKM27_14475"
     CDS             complement(<1..919)
                     /locus_tag="PKM27_14475"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNR08522.1"
                     /translation="MGESKVNQPNSTKELQKFTKALLQDVKSFEYMLAHNWFESGIHR
                     IGAEQEMVILDLKNYRPAPINTKVTPKLEAYDWLDTELAMFNLETNFKPRELKGTCFS
                     EMFEEALGQLKIVREALEPHQADILLTGIIPTIRKSDLDYKYLTPKKRYLALMEAIRK
                     QLSTDSFELRLSGIDELLLKHDSPLLEACNTSFQVHLQVSPDQFARFYNIAQVLAGPM
                     IAIAANSPLVFGRRLWHESRIAMFQQALDTRGSRAHMRERSARVHFGRDWVNDSILEI
                     YKEDIVRFRVLLATEINEDSLNMIHHGRVP"
     gene            1158..2786
                     /locus_tag="PKM27_14480"
     CDS             1158..2786
                     /locus_tag="PKM27_14480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNR08523.1"
                     /translation="MNNLKYRAAVSAFLIFLSFNAAFSQSGSDSSLLLSRGLLLFIFL
                     LLFGLIILISEKMLQIQAVRSGIKDATRYSILPEFSSWFRKKPKAYVGEAPVTYLNKG
                     FDLALTGQPKQDLVEARVNRYAVNATDFRGLPPIPKVVVTVGQEVAAGDPLFYDKNNT
                     EVLYVAPVSGEIIEINRGEKRAITTVVILADKKNRYRSLPPFDLGSSSREQIVKYLCE
                     GGVWPMIKERPFGTIASLDHAPDQIFISCFDSAPLAPDLDYVIRGKEGEFQKGLEVLS
                     RLTSGQVHLGLRANTDEAPAKAFTQASGVEKHWFSGKHPAGNVGIQIHHTAPIRKGGR
                     VWTLGVQEVVSIGALFLRQRHEADRVVALGGSAFTQTGYVQTYIGASLGDLTQGRLIE
                     NSRLISGDVYSGKQESAQGYLHYYDDQVSAIPEGNHETLFGWAIPRIMPSASGVFPNK
                     LLGDISYDADTNTNGEPRAFVMTGQYEAVLPMDIYPQLLVKAIITKDIERMEGMGILE
                     LSEEDIALCEFACTSKQPLQAILREGLDLIQSES"
     gene            2825..4081
                     /locus_tag="PKM27_14485"
     CDS             2825..4081
                     /locus_tag="PKM27_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HNR08524.1"
                     /translation="MALIDKIKSLEPDKKKHPFLHTVYDGFFTFLYQPATVTRGGVHI
                     RDGIDLKRTMAMVVVAMQLCYLFGTYNIGQQYFMAIGKHAGYFEAIHLKILFGLVKIL
                     PIWIVTHLVGLGVELYFAYKKGHGIEEGFLVTGALIPLIMPPDIPLWILAIAVAFAVL
                     FAKEAFGGTGMNIWNVALVARVFVFFAYPGTISGDSVWVAGLTSSNGQVVTDFGGSGW
                     VLHLFDKLWASLGWSTFGADGVPVDGFSGATPLALAAAGGWDAVTQHYTPSEMWWGVI
                     PGSVGETSKPLIIVGALMLIFMRIASWRIMVSMVFGLAITALIFNAWGFNPYMEVPWY
                     YHFYMGSFLFAMAFMATDPVTASATNTGKWIYGFFIGVIGMIVRILNPAYPEGWMLAI
                     LFMNSFAPLIDYFIVESNMKKRLRRV"
     gene            4074..4787
                     /gene="nqrC"
                     /locus_tag="PKM27_14490"
     CDS             4074..4787
                     /gene="nqrC"
                     /locus_tag="PKM27_14490"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HNR08525.1"
                     /translation="MFNNRYILIYTLVMALVVSVALAFVVNGLKPLHDINEAVFKKKD
                     ILSAIRDQIGADPLKMKDEELIRLFEEKMDKVVLDANGKVVTGLDAETIDLAAEEKKP
                     SNERKYPLFIYKGDKGNIYLMSVRGNGLWDKIWGYIALEGDFNTIAGASFGHVAETPG
                     LGAEIKDNANFPNAFHGKKIYENGNYVSVQVVKGGIKVPDHQVDAISGATITSNGVSE
                     MLNRGMVAYLPYFESLKKK"
     gene            4813..5520
                     /locus_tag="PKM27_14495"
     CDS             4813..5520
                     /locus_tag="PKM27_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNR08526.1"
                     /translation="MSESAVKSAASAEPREPWFGKKEKALLSDPLYIDNPITVQVLGV
                     CSALAVTTMVKPALIMGISVALVTGFSNLVVSLLRNGIPKQIRMIVQLVVIAALVTVV
                     EQFLKAFSYDVWKQLSVFIGLIITNCIVMGRLEAFAMANKPWKSFLDGIGNGLGYGWI
                     LVAVAAIREFFGRGALMGFKIIGPSSDFLINTQSSPLLSWYQPNGLMVLPAAAMFLIA
                     TIIWIQRSRTQKLIDIS"
     gene            5543..6151
                     /gene="nqrE"
                     /locus_tag="PKM27_14500"
     CDS             5543..6151
                     /gene="nqrE"
                     /locus_tag="PKM27_14500"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNR08527.1"
                     /translation="MDLLNIFLKSAFIENMVLSYFLGMCSFLAVSKSVKTAIGLGAAV
                     IFVLAITVPMNWLISELVLGPKGIFKMDLTFLRFILFIASIAAMVQLVEMAVEKFSPS
                     LYNALGIFLPLITVNCAILGAALFMVAREYNFSESVVYGVGSGFGWFLAIVLMAAIRE
                     KLRYSHVPAPLRGLGIAFVITAFLGIAFMSLMGINPANYFAK"
     gene            6171..7493
                     /gene="nqrF"
                     /locus_tag="PKM27_14505"
     CDS             6171..7493
                     /gene="nqrF"
                     /locus_tag="PKM27_14505"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNR08528.1"
                     /translation="MIFLIDLMFVVWAIVAFSALLLLLAFGLIYAQKKLVAQGDVKII
                     INGDEEHPLKVKPGSSLLSTLAGEKIFLPSACGGGGTCAMCECHVDQGGGDILPTELN
                     HISRKEAAEHMRLACQVKVRQDMHIRVPAEVFGIKKWECEVVSNYNVATFIKEFVVKL
                     PEGESLDFQSGGYIQIDVPPIEVAYKDFDIKPHPSDPAGPDKFKPDWDKFNLWPLRMK
                     NSEEVFRAYSMANHPAEGNIVKLNIRIATPPWDKKTNGWMAVNPGICSSYIFSRKPGD
                     KVMVSGPYGEFFIKPTKKEMVYIGGGAGMAPLRSHLFHLFHTLKTTDRKVSFWYGGRS
                     KRELFYTDEFRAIEKEFPNFRYYIALSEPMAEDNWKLSKDLEDREGDGFVGFIHKVLY
                     DNYLKNHPEPEEIEYYFCGPPMMNAAVIKLLDDLGVPKENIAFDDFGG"
     gene            complement(7595..8902)
                     /locus_tag="PKM27_14510"
     CDS             complement(7595..8902)
                     /locus_tag="PKM27_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNR08529.1"
                     /translation="MSNYRFETLQLHAGQQVDPTTKSRAVPIYQTTSYGFDNSDHAAN
                     LFGLRQFGNIYTRIMNPTTDVFEQRMAALEGGVAALATASGQSAQFLALNNIMQAGDN
                     FVSTSYLYGGTYNQFKVAFKRLGIEARFADGDSPASFEKLIDQNTKALYLETIGNPKL
                     NIPDFEAISALCKKYDLPLIVDNTFGAGGYLFQPLRHGAHVVVNSATKWIGGHGTSIG
                     GVIVDGGNYNWGNGKYPQFTEPSEGYHGMKFWEIFGDNNPLGMPNVAFIIRARVEGLR
                     DFGPSLSPFNSFLFLQGIETLSLRVQRHVDNAMALATWLENHDKVEFVWYPGLKSSPY
                     HELAKKYLSNGFGGILQFGIKGGLENGKKFIDSLQLISHLANVGDAKSLAIHPASTTH
                     EQLSDTEREAAGVLPNLVRISAGLEHIEDIKADFQQAFDKVFA"
     gene            9190..11382
                     /gene="metG"
                     /locus_tag="PKM27_14515"
     CDS             9190..11382
                     /gene="metG"
                     /locus_tag="PKM27_14515"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNR08530.1"
                     /translation="MTTAWIGRIVHFLTFTAQRMEIKRYLVTSALPYANGPLHIGHLT
                     GAYLPADIYVRFLRLTGKEVVWVCGSDEHGAAITVKARKENTTPGAIIDKYHEQLKMA
                     LAGMAIDFNIYHRTSEPIHHETSQDFFRTLYEKGEFQEIESQQYYDESAGQFLADRYI
                     MGTCPKCGNPNAYGDQCEKCGSTLSPTELINPRSTLSGTIPVMKPTTHWYLPLNQHQE
                     WLKTWIETGMLEGVKHHEPETWKPHVLGQCKSWLDSGLEPRSMTRDLDWGVDVPKEIP
                     GSEGKKLYVWLDAPIGYISATKAWAATHHETWEKYWKDPETALIHFIGKDNIVFHCLI
                     FPVILKSYGGYNLPINVPANQFMNLEGDKVSTSRNWAVWIHEYLEEWPGQQDLLRYYL
                     IKTMPEQRDSEFTWKGFQEANNNELVNNLANFFNRVCVLLHKYYQGEIPAFNPDKAFV
                     GTEEPDFDSFHETELLYLHDRIQDLNQSIREFRFRDALKSLMDMSSTGNSILQSNEPW
                     KKEQSDPELIKVVINYCAQLATALSVVIRPFMPFTSDKMRAQLGLSPLRDEDELNQLL
                     DALSQGEVLLPPGHKINEASHLFSRIEDARIQAQLDKLKRGAALSAPAPAVHVPLKET
                     ISIDDFNKTDLRAGTILTADRVPKADKLLHLTVDLGFEKRTILSGIAQHFQPEVLPGR
                     RVVIVANLAPRKMKGIESNGMILLSENGDGSLRFVEPSDGAENGSVVK"
     gene            12108..12827
                     /locus_tag="PKM27_14520"
                     /pseudo
     CDS             12108..12827
                     /locus_tag="PKM27_14520"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
     gene            13044..>19506
                     /locus_tag="PKM27_14525"
     CDS             13044..>19506
                     /locus_tag="PKM27_14525"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNR08531.1"
                     /translation="MHPGATEICDEVDNDCDGFIDEDLATTWYQDSDGDGFGNPGVTK
                     DSCAMPSGFVNNTTDCNDSEPAAYTGAVEICDDGIDNDCDSYTDCQDGDCNGIGNSMM
                     ASSNTYYADTDEDGYGDPAITKDSCALPIGYVADNTDCNDECYLCNPVATEICDGLDN
                     DCDGLVDDSLDTYFADFDGDNFYNPGISKDTCAQPVGFISLNEAAGIDCNDGDDQLTN
                     NCPSITYYVDSSNTDVMALTGRFRSALLVGESWDMAFTTLQAALATAVAGDTIKVAQG
                     TYYPGSTRDSSFIIPSGVVLLGGYPMGGGTDEERNWDCYKTILSGDIGNPDNNSDNSY
                     HVVVTTNTDSSTQVDGFCITGGNANGTFDDRYGGGWFNRGTGGDSSNPTIIHCRIFGN
                     NAARNGGGIGNFGDLGPTNPRIINSIISGNTATRGAGIYNYGYGSNSSPVLINCILSG
                     NRANYAGGGIYNDGEDGNSSPILINCVLNGNYAGSFGSAAFNTGYDGDMTGNSDPFFV
                     NCVIWNNDAGIAQNVFFNEDANLTVSYSVLQGTDFASITDFGYNATSGIANTFSDPAF
                     INAPASATAPTTAGDFHVLVYSPCINHGDNASNSFPTDLDDLARIYNGTIDAGAYEFQ
                     SIPCSGFTGVAYVNASAAGLNNGSSWTDAFTDLQDALAIARTCGLDTILVAEGTYYPS
                     TTDTLYDCAGNIESIIQLDSSLSFDIPDSLVMIGGYQVMNGIATGRDPWCFKTLLCGD
                     IDQDGEVYGDLQNSMHVVTTSQVSNATVVDGFHISGGNASESYPNYPDYVGGGWYDDG
                     SGMNGGSFPTILNCVISGNWANGGGGIFCDGTQGGNSSPRLINCILSGNGANASGGAM
                     FNDGNTGTSSPSLDNCVVSGNIAYYQWGGGMYNSGINSGVCAPQIRNSIFWNNFADIS
                     GPVFHNNDAVTTVSNSILQGSTDLNDGSVSSNENGGTTTGSDNLFSDPLFINAPAAAV
                     PPTTAGDFRVASYSPAVNFGDDAAIMAALSGHAFIDLNGNPRIAGAGVDAGVFEYQGT
                     DCTPYSGINTLYVDAQAAGSETGADWPNALLTLKEAIEIATYCTGIDSILVAAGTYYP
                     SCPDTVYDPCTGAVQTVLAPSRQQSFILPDGITLLGGYPTGGIGERDPHCNLTILSGD
                     IDMNGDSTGNTFNIVKTIEYQDTIVFDGFIIEQGNAIANGEEGGAWWDGSRTDSALLC
                     ISNTIFRENYAVLGGAFYGEGTRDLKILASEFYENTAAERGGAVYFQPVDNMFDSAFT
                     RMENVLLHHNTASMNGGAIAISFHPNISLDIIHATFSDNSPDVLNKATVGPARIRNSI
                     IWNNISPVFTNFNLPDVQFSIVQDGFSGTGNKFEDPLFRQPGFDYRLQYASPAIDMGD
                     NVFSMETTDLDDHARIQNGTTDMGAYEFQAPVCSGSGTAYVDSSAEAGGDGTSWETAF
                     NDLQQALVLAYFCQVDTILVAQGTYYPSRPDSVFDCRGALDTVYAANRDISFNIPDSI
                     TLLGGFPTGGGTLEERDWECNKTILCGEIQQDGDHTNNSYHVVTTIGVDSITTVDGFC
                     IQDGYAVYTSFENFPTFRNVGGGWLNIGVNIFIQLDDMAIADYYSNPRILNCTFYNNN
                     GLLGAGLGNLGFLSNASPLIYNCNFNSDSAILGGAIANIGISGESSPYIVNTKIQGNT
                     GLAAGGILNLGGSVQLIFGLQGIRTQSDQPIKMDSDNAQVLKGLIQSGFLGQLGSVNQ
                     NRNAGPEGAQYLIDPEYGVSAPVIINSLITGNAAAAGGGMVNAVLTLANEEFIGLSGE
                     SSPRLINCDITGNLAEIGAGILNTDFILDLTNGLSPVEGGGRLRPCIENSILWNNDAG
                     GDGNDIANMFDEVSDSIKLHHSITGDNYLDLNHNCIECVDTLLGGNKLATDPLFVDAP
                     PGGAAATTLGNFHLQDLSPAINMGENSVNPLSTDLDGLARIVQAVIDIGAYEKHLFCT
                     DDTLYVGVDGSLPPVEVILEETLPDGYELIVNGLADEDGMIELNCSDTGLNELVIEIV
                     RGCIPVLVDSCHMNITILDTFEKNLVCNDLVNVSLGAGCLKVLDPDDFLENIRGCRGL
                     YDIQVIYPLGTHRFPGEDQVDLSHIGYTMVYNVYEVGTGNKCWGYLKVEDKQPPLLNC
                     SVDTITCYELPDLPLLAEGADS"
BASE COUNT         4786 a         4838 c         5149 g         4733 t
ORIGIN      
        1 tgggcacgcg gccgtgatgg atcatattga gagaatcttc attgatctcg gtggccagca
       61 atacccggaa ccgcacaata tcttctttat aaatttccag gatcgaatca ttgacccagt
      121 cccggccgaa atggacccgg gcgctgcgtt cccgcatatg cgccctggaa ccgcgggtat
      181 ccagggcttg ctggaacatg gcgatccggg attcatgcca gagccttcgg ccaaagacca
      241 ggggcgagtt ggccgcaatg gcgatcatcg gcccggccag aacctgtgcg atgttgtaaa
      301 accgggcaaa ctggtccggg gagacttgca ggtgaacctg gaaactggtg ttgcaggcct
      361 ccagcaaggg ggagtcgtgc ttgaggagca attcgtcaat gccgctcagc cggagctcaa
      421 aagaatcggt cgacaattgt ttccggatgg cttccatcag ggcgagatat ctttttttcg
      481 gggtcaggta cttatagtca aggtctgatt ttcttatggt cgggatgatg cccgtgagca
      541 ggatgtccgc ctggtggggt tcgagcgcct ctcttacgat tttcagttgg ccgagggctt
      601 cctcaaacat ttcggaaaaa caagtcccct tcagttcccg cggcttaaaa ttggtttcca
      661 ggttaaacat ggcgagttcc gtgtccaacc agtcataagc ttccagtttt ggggtcacct
      721 tggtattgat gggagcaggg cggtagtttt tcaggtcaag gatgaccatc tcctgttctg
      781 ccccgatgcg gtgaatcccg gattcaaacc aattatgagc cagcatgtat tcgaaagatt
      841 tgacgtcctg gagcagggct ttggtaaatt tttgcagctc tttggtactg ttgggttggt
      901 ttactttact ctctcccata attcattata tcacggtgaa aagtaccaat aaaaattcag
      961 ctttccgtgt tccggatcct accctggggt tcagcccatc aaggacaatt cagggagatc
     1021 atatcccaaa acctatgtaa tttagactaa ttctaaatag ataaagccct ttcaactagc
     1081 catgatatat tatatgaaat tttatttggt ataaagtcaa atagtaactt tgcggcgcac
     1141 gattaaatca tgtacctgtg aataatctaa aataccgggc agcggtttct gcttttttga
     1201 ttttcctttc ttttaatgct gctttttccc aatccggttc ggacagcagc ctgctgctga
     1261 gcaggggttt gcttttgttt attttcctgc tcctgtttgg cctcattatc ctgatatccg
     1321 aaaaaatgct tcagatccag gcggtccgtt ccgggataaa ggatgccacc cgttattcga
     1381 tccttcccga attctccagt tggttccgta aaaaaccaaa ggcttatgtg ggagaggccc
     1441 cggtcactta tctcaataaa ggatttgacc tggctcttac cggacaacca aaacaggatc
     1501 ttgtggaagc gcgggtaaac cggtatgccg tcaacgcgac ggatttccgc ggccttcccc
     1561 ccatccccaa ggtcgtggtt accgtgggcc aggaagtagc cgcgggcgac ccgctttttt
     1621 atgacaaaaa caataccgaa gtgctttatg tcgctccggt gagcggagaa atcattgaaa
     1681 tcaaccgggg tgaaaaaagg gccattacga cggtggtgat cctggctgat aaaaaaaacc
     1741 ggtacagatc attgcctcct ttcgacctgg gatcttcaag ccgggagcaa attgtaaaat
     1801 acctgtgtga aggtggcgta tggcccatga tcaaggaaag acctttcggt acaattgcca
     1861 gcctcgacca tgcccccgac cagattttca tttcgtgttt tgattccgca cccctcgctc
     1921 ctgacctgga ttatgtcatt cgcgggaagg agggggaatt tcagaaaggt ttggaggtct
     1981 tgagccggct tacctcaggc caggttcatc tcggactccg tgcaaatacg gatgaagccc
     2041 cggcaaaggc cttcacccag gcctcggggg tggaaaaaca ctggtttagc ggcaaacacc
     2101 cggccggcaa cgtcgggatc caaatccatc ataccgctcc aataagaaaa ggagggaggg
     2161 tgtggacgct gggagtacag gaagtggtca gcattggggc cctctttctc cgccagcggc
     2221 atgaggcgga ccgggtcgtg gcgctcgggg gaagtgcctt tacccaaacc ggatatgttc
     2281 agacttatat tggagcctcc ctcggggacc tgacccaggg caggcttata gaaaacagca
     2341 ggctcattag cggcgatgta tacagcggca aacaagaaag tgcccaggga taccttcatt
     2401 actacgatga ccaggtatcc gccattcccg agggtaacca cgaaacgctc tttggatggg
     2461 ccatcccccg gatcatgccg agtgcttccg gagtctttcc gaataaattg cttggggata
     2521 tttcatatga tgcggatacc aacaccaatg gagaaccccg cgcttttgtc atgaccggcc
     2581 agtacgaagc ggtgcttccc atggatatat acccgcagtt gctcgtcaaa gcgatcatta
     2641 ccaaggatat tgagcgcatg gaaggcatgg gcatcctgga actgtccgaa gaagacatcg
     2701 ccctctgcga atttgcctgt acttcaaaac aacccttgca ggcaatcctc cgggaaggcc
     2761 tggacctgat tcagtctgaa agttaatgaa cctttgaaac caagaattat cacgatatac
     2821 ggatatggcc ttaatcgata aaatcaagtc actggaaccg gataagaaaa agcatccgtt
     2881 tttacacacc gtttacgacg gttttttcac ctttttgtat cagccggcca cggtcacccg
     2941 gggcggggtc catatccgag acgggatcga cctcaaaagg accatggcta tggtggtggt
     3001 ggccatgcaa ctctgttatt tgtttggaac ctataatatc ggccagcagt attttatggc
     3061 catcggcaaa catgccggtt attttgaagc catccacctc aaaatccttt ttggcctggt
     3121 caaaatcctg cccatctgga ttgtcaccca cctggtgggc ctgggcgtag agctttattt
     3181 cgcttataaa aaggggcatg gcatcgaaga gggtttcctg gtgaccgggg ccctgatccc
     3241 cctgatcatg ccgccggata tcccgctatg gatcctggcc atcgcggtgg cctttgcggt
     3301 tcttttcgcc aaggaggcct tcgggggtac gggcatgaac atctggaatg tagccctggt
     3361 ggcccgggtc tttgtctttt ttgcttaccc tggaaccatt tcaggggaca gtgtttgggt
     3421 agccggcctt acatcttcca atggccaggt cgtcacggat ttcggaggtt ccggttgggt
     3481 cctccacctt tttgataaac tttgggcctc cctgggatgg agcacttttg gagccgatgg
     3541 ggtcccagtg gacggattca gcggtgccac ccccctggca cttgccgcag caggaggatg
     3601 ggatgcggta acccagcatt acactccttc cgagatgtgg tggggcgtga ttcccggttc
     3661 ggtcggggag acctctaaac ccctgatcat cgtcggggcc ctgatgctga tcttcatgcg
     3721 cattgccagc tggcggatca tggtgtccat ggtgtttggc ctggcgatca ccgccctgat
     3781 ctttaatgcc tggggattta atccctatat ggaagtcccc tggtactacc acttttacat
     3841 ggggagtttc ctctttgcca tggccttcat ggccaccgac ccggtcaccg cttctgccac
     3901 gaacaccggt aaatggatct atggcttttt tatcggggtg atcggtatga tcgtacggat
     3961 cctgaacccg gcctacccgg aaggctggat gctggcgatc ctgtttatga actcctttgc
     4021 gcccctgatc gattatttta ttgttgaatc caatatgaaa aaaagattgc gccgtgttta
     4081 acaaccgata tatcctgatc tataccctgg tgatggccct ggtcgtatcc gtggccctcg
     4141 cttttgtggt caatggcctc aagccgctcc atgatatcaa cgaagcggta ttcaagaaaa
     4201 aggacatcct cagcgccatc cgcgatcaga tcggcgccga tccgttaaaa atgaaggacg
     4261 aagagctgat ccggcttttc gaagaaaaaa tggataaggt ggttctggat gccaatggaa
     4321 aggtggtgac cgggcttgac gccgaaacca ttgacctggc tgcagaagaa aaaaagccgt
     4381 ccaacgagcg caaatatccc ttgtttattt acaaagggga caaaggaaac atttacctca
     4441 tgtccgtccg cggcaatgga ctctgggata agatctgggg atatattgcc ctggaaggcg
     4501 acttcaacac tattgccgga gcctccttcg ggcacgtcgc ggagaccccg ggactgggtg
     4561 ccgagatcaa agacaacgca aacttcccca atgcctttca tggaaaaaag atctatgaaa
     4621 acggcaatta tgtatccgtc caggtggtca aaggaggaat taaagttccc gatcaccagg
     4681 tggatgctat ttccggggct accatcacct ctaatggggt atccgaaatg ctgaaccggg
     4741 ggatggtcgc ctatttgccc tattttgaaa gtttaaaaaa gaaataattc aaaagaagaa
     4801 aaacaaagcg atatgtctga atccgctgtt aaatccgctg cttctgccga acccagggaa
     4861 ccctggtttg gcaaaaagga aaaggccctc ctttcggacc ccctgtatat cgataacccg
     4921 atcacggtgc aggtcctggg cgtttgttcc gcactggcag tgaccaccat ggtcaaaccg
     4981 gcactcatca tgggtatttc ggtggcgttg gtgaccggct tttccaacct ggtggtttcc
     5041 ctgcttcgga acgggatccc aaaacaaatt cggatgattg tccagctggt ggtcattgcc
     5101 gctctggtga ccgtagtcga acaatttcta aaggcttttt cctacgatgt gtggaaacag
     5161 ctttccgttt tcatcgggct gatcatcacc aactgcatcg tgatgggccg gctggaggct
     5221 tttgccatgg ccaacaaacc ctggaaatcc ttcctggatg gaatcggtaa cggactgggt
     5281 tatggctgga tcctggtggc ggtagccgcg atccgggaat ttttcggacg cggtgccctg
     5341 atgggcttca agatcattgg accttcctcc gattttttga tcaataccca gtcttcgccc
     5401 ttattgagct ggtaccagcc caacggcctt atggttttac cggctgcggc gatgtttctg
     5461 atagccacca tcatctggat tcaacggagc cggacgcaaa agctaatcga tatttcctaa
     5521 tgacccatta attcccagac tcatggacct cctcaatatt tttctcaaat ccgctttcat
     5581 cgaaaacatg gtgctttcct attttttggg aatgtgctcc ttcctggcgg tgtccaaaag
     5641 cgtgaaaacc gccatcgggc ttggggccgc cgtcattttt gtgctggcca ttaccgtgcc
     5701 gatgaactgg ttgatcagtg aacttgtact cggaccgaaa gggatattca aaatggatct
     5761 gactttcctc cgcttcatcc ttttcatcgc cagcatcgcg gcgatggtcc aattggtgga
     5821 aatggccgtt gaaaaatttt ccccttccct ctataatgca ctgggtattt tccttcccct
     5881 gattacggtc aactgtgcaa tcctcggtgc ggccctcttt atggtcgccc gggagtataa
     5941 cttttctgaa tcggtggttt atggcgtggg ttccggcttt ggctggttcc tggctatcgt
     6001 actgatggcg gccatccgtg aaaaactgag atacagccac gtgcctgcgc ctttgcgcgg
     6061 actgggtatc gccttcgtga tcaccgcctt cctgggcata gcctttatga gcctcatggg
     6121 catcaacccg gcaaattatt ttgctaaata actttaattc aatgactatc atgatatttc
     6181 tgatcgacct gatgtttgtg gtttgggcga ttgttgcttt ttcggcctta ttgcttttgc
     6241 tggcctttgg cttgatttat gcccagaaaa aactggtggc ccagggggat gtgaaaatca
     6301 tcattaacgg ggacgaggaa catccgctca aagtaaagcc gggcagcagc ctgctgagta
     6361 ccctggccgg tgaaaaaatc tttcttcctt ccgcctgcgg gggaggaggc acctgcgcca
     6421 tgtgcgaatg ccatgtcgac cagggaggag gggacatcct gcctacggaa ctgaaccata
     6481 tttcccgcaa ggaggccgcg gaacacatgc ggctcgcctg ccaggtcaaa gtcaggcagg
     6541 atatgcatat ccgtgtgccc gcggaagtct tcgggatcaa aaaatgggag tgtgaggtag
     6601 tcagcaatta caacgtggcg accttcatca aagaatttgt ggtaaaactt ccggaagggg
     6661 aatccctgga cttccagtcg ggcggataca tccagattga cgtgccgccc atcgaagtgg
     6721 cttacaagga ttttgacatc aaaccccatc cttccgatcc cgccggaccg gataaattca
     6781 agccggactg ggacaaattc aacttatggc ccctgcgcat gaaaaatagc gaagaggtgt
     6841 tcagggccta ttccatggcc aaccaccccg ccgagggaaa tatcgtaaaa ctgaatatcc
     6901 gtattgccac gccaccctgg gataaaaaaa caaatggctg gatggcggtc aaccccggca
     6961 tctgctccag ttatattttt tcccggaaac cgggagacaa agtgatggtg agcggacctt
     7021 acggagaatt tttcatcaaa ccgaccaaaa aggaaatggt gtatatcggg ggcggcgccg
     7081 gcatggctcc cctgcgttcc catttgttcc acctttttca taccctgaaa accacggacc
     7141 gcaaagtcag tttttggtac gggggacgct ccaaacgcga attgttttat acagatgaat
     7201 tcagggccat tgaaaaagaa tttcccaatt tccgctatta tatcgcgctt tccgagccca
     7261 tggccgaaga caactggaaa ttaagcaagg acctcgaaga ccgcgagggc gacggttttg
     7321 tgggctttat tcacaaggtg ctctacgaca actatctgaa aaaccatccg gaacccgaag
     7381 agatcgaata ctatttctgc ggtcctccca tgatgaatgc ggcggtgatc aaactgctgg
     7441 atgatcttgg ggtacctaag gagaatattg ccttcgacga tttcggagga taataaaaag
     7501 ggggaatcaa aagttccccc tttttttttt gtcatcagcc atcagccatc agccatcagc
     7561 catgattttt atcattgcag atagctgaaa tctattaagc aaaaactttg tcaaaagcct
     7621 gctggaaatc ggctttgatg tcttcgatat gctccaggcc tgcactgatc cggaccaggt
     7681 tgggtaatac gccggctgcc tcgcgctcgg tatcactcaa ttgctcgtgc gtagtggagg
     7741 cgggatgaat ggccagtgac ttggcatcgc cgacgttggc caggtggctg atcaattgca
     7801 gggaatcgat aaacttcttc ccgttttcca gaccaccctt gatgccaaac tggaggatcc
     7861 cgccaaaacc gttggacagg tattttttgg ccaactcatg gtaagggctg ctttttaaac
     7921 cgggatacca gacaaattcg accttgtcgt ggttttccag ccaggtagcc agggccatgg
     7981 cattgtccac gtgacgctgg acccggaggc tcagggtctc gatgccctgc aaaaagagga
     8041 aggaattgaa ggggctgagc gagggcccga aatcacgcag tccctccacc cgcgcacgga
     8101 tgataaaggc tacattgggc ataccgaggg gattgttgtc tccgaagatc tcccagaatt
     8161 tcatgccgtg ataaccttcg ctgggttcgg tgaattgggg atattttcca ttgccccagt
     8221 tgtaattgcc cccgtccacg atgaccccgc ctatgctggt tccgtgtccg ccgatccact
     8281 tggtggcgga attcaccacg acatgggctc cgtgccggag gggctggaag agatatcccc
     8341 cggcgccaaa agtattatcc acgatcaggg gaaggtcgta tttcttgcaa agggccgaga
     8401 tggcttcaaa atcgggaatg ttcagtttgg ggttgccaat ggtttcgagg taaagggcct
     8461 tggtgttttg gtcgatcagt ttttcaaagc tggcggggct gtccccatcc gcaaaccggg
     8521 cttcgatgcc cagcctttta aaggccactt taaattggtt gtaggtgccc ccgtacagat
     8581 aggaggtgga gacaaaattg tcgcccgcct gcatgatgtt gttcagggca aggaattggg
     8641 ccgactggcc ggaagcggtg gccagggcgg ctaccccgcc ttccaaggct gccattcgtt
     8701 gttcgaatac atccgtggtg gggttcatga tgcgggtata gatgttgcca aactggcgaa
     8761 ggccgaaaag gttggcggca tggtccgaat tgtcaaaacc ataggaagtg gtctggtaga
     8821 tcggcactgc ccgggatttg gtggtcgggt ctacctgttg tcctgcgtgc aattgcaggg
     8881 tttcaaatct gtaattagac ataaatttta aaaattaaaa ggttgatgat atgattgaat
     8941 gaataagttc gaaaataagg gggtaagaat tggtccggga aaatggggta atatggatca
     9001 cagcatgcct tacggcatac aacagataca aacgacggcc acgggtttat ccattttcac
     9061 taccaaatat aattaaaagg cggaatccgg gaacaagagc ctataaattc tatcgttcag
     9121 gtatggtttg gaagggtgga tggtgggtgg tggatggtga gtttctatac cggacctcct
     9181 taaggtaggg tgacgacggc atggataggg agaatcgtcc attttcttac ctttacggcc
     9241 caacggatgg agataaaaag atacctggtc acttcggcct tgccttatgc caacggcccc
     9301 ttgcacatcg ggcatctcac cggggcctat ttgcctgccg atatctatgt acgttttcta
     9361 aggctgaccg ggaaggaagt cgtgtgggtc tgcggttcgg atgaacacgg cgccgccatc
     9421 acagtcaagg cacgaaagga aaacacgacc ccgggggcca tcatcgataa ataccacgaa
     9481 caactgaaaa tggcgctggc cggcatggcc attgatttta atatctatca ccggacttcc
     9541 gagcccatcc accatgaaac ctcgcaggat tttttcagga ccctctatga aaaaggcgaa
     9601 ttccaggaaa tcgaaagcca gcaatattac gatgagtccg ccggccagtt ccttgcggac
     9661 cggtacatca tgggtacctg ccccaaatgc ggcaatccca acgcttacgg cgaccagtgt
     9721 gaaaaatgcg gatcgacgct gagcccgaca gaactgatca atcccaggtc caccctcagc
     9781 gggaccatcc cggtgatgaa acccaccacg cactggtacc ttccactcaa tcagcaccag
     9841 gaatggttaa agacctggat cgagaccggc atgctggagg gggtcaaaca ccatgaaccc
     9901 gaaacctgga agccccatgt gcttggccaa tgcaagtcct ggctggacag cggcctggaa
     9961 ccccggtcca tgacccgtga cctcgactgg ggagtggacg ttccgaagga gatccccggc
    10021 tctgaaggta aaaaacttta tgtctggctg gatgccccga tcggttatat cagcgctacc
    10081 aaagcctggg ctgccaccca ccatgaaacc tgggaaaaat actggaagga cccggaaacc
    10141 gccctgatcc attttatcgg aaaagacaat atcgtctttc actgcctcat ctttccggtt
    10201 atcctcaaat cctatggcgg gtataacctt cccatcaatg ttccggcaaa ccagtttatg
    10261 aacctggagg gggacaaggt ttccacttcc agaaactggg cggtctggat ccacgaatac
    10321 ctggaggaat ggcccgggca gcaggatctc ctgcggtatt acctcatcaa gaccatgccc
    10381 gagcagcggg acagcgaatt tacctggaaa ggcttccagg aagccaacaa caatgaactg
    10441 gtcaacaacc tggctaattt tttcaaccgg gtctgtgtcc tgctgcacaa atattaccaa
    10501 ggggaaatcc ctgcctttaa tccggataag gcctttgtgg gtacagaaga gcctgatttt
    10561 gattccttcc atgagacgga attgctctat ctccacgacc ggatccagga cctcaatcaa
    10621 agcatccggg aattccggtt cagggatgcc cttaaaagcc tgatggatat gtccagcacg
    10681 ggcaattcca tcctacagtc caatgaaccc tggaaaaagg aacaatcgga tcctgaatta
    10741 atcaaggtcg tgatcaatta ttgcgcccaa ctggccaccg cgctcagtgt ggtcatccgg
    10801 ccttttatgc cctttacctc ggataaaatg agggcccaac tcggtctttc tccgctccgg
    10861 gatgaagatg agctaaacca attgctggac gccttgagcc agggggaagt cctcctccct
    10921 cccgggcata agatcaacga agccagccat ctgttctcaa ggattgaaga tgcacgcatc
    10981 caggcccagc tggacaagct aaaaagaggg gcagcgctca gcgctccagc acctgctgtt
    11041 catgtcccgc ttaaagagac catatccatc gacgatttca acaaaacaga tctccgggca
    11101 gggaccatcc tgacggccga ccgcgtgccc aaggcggata aattattgca tctgacggtt
    11161 gacctcggct ttgaaaagcg taccatactc agcggtatag cccagcattt tcagcctgaa
    11221 gtcttgcccg gtcgcagggt agtcatcgtc gccaatttgg caccccggaa aatgaaaggg
    11281 atcgaatcca acggcatgat cctgctctcg gagaatgggg acggcagcct gcggtttgta
    11341 gaaccgagtg atggggcgga gaatgggagc gttgtcaaat aattaaataa ttaaataatt
    11401 aagaaattaa gaattaaggg gagccgtctc aaccttaacc ttaaccatac cagttaaaat
    11461 tctgatgtaa aaattcctca ttcagagtta tttagattaa gtaactcttt ttaccttcga
    11521 ttaaatttcc cttagtgaac ccgtctcagc tgacagtatt tgccgcaagc ccttgcgtgc
    11581 attttccctg gtacggcttt cgctccaatc caaattacca accctttaac taagtattca
    11641 tgtaccggaa actgattatt tcatttacta ctgctctggc gcttggctcc tggttcttat
    11701 tcacaaacaa tgaagatgtc ccagatcagg cattcgcagt cgttaaaaca gattgtgagg
    11761 aatacggcgg ggattgggac ggggacggag tctgcgcgga ttttgactgc gacgacagcg
    11821 atgcggagaa tactaaggtg cagcccgact gcggggataa ctgtacgaac ggcggaagtt
    11881 cgggagtgga tgccgatagc gatggctatt gcaatgatgt ggattgcgat gatgctgatg
    11941 atagcgtatg ggatttactg gatccagctt acatcgataa tgatggagac ggatacgatg
    12001 acggcacaac tacagtatgt tggaatggat tcgatttgcc cagtggctac cgtgatttta
    12061 caaacgggac ggactgtgat gactatgatg atctcgtcta taccaacatg gaattatata
    12121 ccgatttcga ccaggacggg tatgacgacg gatcgggaac caccacactt tgcacggatg
    12181 gcaacgctcc atccggttat attgaaaatt caaatgggac ggactgcgat gataatgatg
    12241 atagtgtatg ggatttatta gatcctgctt atatcgataa tgatggagat ggatacgatg
    12301 acggcaccac tactgtttgt tggaatggga cgaaccctcc gacaggatac agtgtaaata
    12361 cttccggtac ggattgtgac gacactgacc cgaacgtcaa tcccgatgcc tccgaaattt
    12421 gcaatggcat cgacgacgac tgtgacggcg agaccgatga ggatcctgtt gatggattca
    12481 cgtattatgc ggatactgac gcagatactt acggggatgc gaacaacacg atctacgcct
    12541 gcacggcccc aaccggatat gtttcagacg atacggattg tgatgacacc aatggtcagg
    12601 ttcatccggg ggcagatgaa tattgcaatg atatcgatga cgattgcgat ggggagttcg
    12661 atgaggatgc catagaccca agcacctggt atgaggatgg agacggagat gggtacggaa
    12721 acccagcggt ctcccagatg gcatgtgacc aacccacggg gtatatttca gacaatacgg
    12781 attgcgatga taccgacttt tatgtaaacc ctggtgttgc ggaaatatgc gacggctacg
    12841 acaacgattg cgatggaacc agcgatgaag gatttggcaa cgacaacgac atggacggcg
    12901 taggcagtga ctgcgacaat tgtcctgacg aatacaatcc accggttgcc tgcctcgcct
    12961 gcctcattcc ggcaagcggc caagctgatg cagacagcga tggttggggt gatgaatgcg
    13021 attgcgatga tgccaacggt cagattcatc cgggagcgac cgaaatctgc gatgaggtgg
    13081 acaacgattg tgacggattc atcgatgaag acctggcaac gacctggtat caggacagcg
    13141 acggagatgg gtttggcaat ccaggtgtca ccaaagacag ttgtgccatg ccttcaggtt
    13201 ttgttaataa cactacagac tgcaatgact ctgaaccggc tgcctatacc ggtgccgtcg
    13261 aaatttgtga cgatggtatc gacaacgact gcgactcata caccgattgc caggatgggg
    13321 attgcaatgg gattggaaat tctatgatgg catcctcgaa tacctactat gccgacaccg
    13381 atgaggatgg ctatggcgat ccggccatta cgaaagacag ttgtgccctc cccataggtt
    13441 atgtcgctga caatacagac tgtaacgacg aatgctatct atgtaacccc gtagctacgg
    13501 agatctgcga cgggctggac aatgattgcg acggtctggt ggacgacagt cttgatactt
    13561 attttgccga ttttgacgga gataattttt ataatcccgg catatccaaa gacacctgcg
    13621 cccagccggt aggttttatt tccctgaatg aggccgccgg cattgattgc aatgacgggg
    13681 acgatcaact gaccaataat tgcccatcga tcacctacta tgtcgattcc tccaataccg
    13741 atgtgatggc gctgaccggc aggttccggt ccgccctcct ggtgggtgaa tcctgggata
    13801 tggcttttac cacccttcag gcggccctgg ccacggcagt ggcgggagat acaatcaagg
    13861 ttgcccaggg aacttattac ccaggcagca cccgggacag ctcattcatc attcccagtg
    13921 gggtcgtgct cctgggcggg tacccgatgg gtggtggtac ggatgaggaa cggaactggg
    13981 attgttacaa gaccatcctg agcggggata ttggaaaccc cgacaacaat tcggacaaca
    14041 gttatcacgt ggtggtcacc accaatacgg acagctcaac ccaggtggat ggattttgca
    14101 tcaccggagg caatgccaac ggtacctttg acgaccgcta cggaggcgga tggttcaaca
    14161 ggggaacggg tggagattca tcaaatccca ccatcattca ttgccggatc tttggaaata
    14221 atgccgcccg aaatggcggg gggatcggca atttcgggga tctggggccc accaatccca
    14281 ggattataaa cagcatcatc agcggaaata cggccactcg gggtgcagga atttacaatt
    14341 atggttacgg atcaaacagc agtccggtgc tcataaactg cattctgagc ggcaaccggg
    14401 ccaattatgc gggaggagga atatacaacg acggggaaga tggcaacagc agtcctatac
    14461 tcatcaattg tgttttaaat ggaaattatg cgggttcttt cggcagcgct gctttcaata
    14521 cgggttatga cggagatatg acaggcaaca gtgatccttt cttcgttaat tgtgtgatct
    14581 ggaataatga tgccggaatt gcccaaaatg ttttctttaa tgaagatgca aatttgacgg
    14641 tttcgtattc agtgctgcaa ggcaccgatt ttgctagcat caccgatttc ggatataatg
    14701 cgaccagcgg aatcgcgaat accttttccg atccggcttt tataaatgct ccagccagcg
    14761 ccactgcccc gaccacggcc ggggatttcc atgtcctggt ctattcccct tgcatcaacc
    14821 atggtgacaa tgcatccaat tcttttccaa cggatctgga tgacctggcc cgcatttata
    14881 acggcaccat cgacgcgggg gcttacgaat ttcaatccat cccttgtagc ggctttaccg
    14941 gtgtagctta tgtcaatgct tcggctgcag gacttaataa cggcagctcc tggacggatg
    15001 ccttcaccga cctccaggat gccctggcga tagccaggac ctgcggactg gacaccattc
    15061 tggtcgcgga agggacctac tatcccagca cgacggatac gctctacgat tgtgcaggaa
    15121 atattgaatc catcattcaa ctggactcga gcctttcctt tgacatcccc gattccctgg
    15181 ttatgatcgg aggataccag gtgatgaatg gaatagccac cggccgggat ccctggtgtt
    15241 tcaaaacgct tctttgcggt gacatagacc aggacgggga agtctatggg gacctgcaaa
    15301 acagtatgca tgtagtaacc acttcacaag tgagcaatgc aacggtggtg gatggattcc
    15361 atatttccgg aggcaatgcc agcgaatctt atccaaacta tcccgactat gtgggagggg
    15421 gttggtatga tgacggcagc ggcatgaatg gaggttcatt cccaaccatc ctcaactgtg
    15481 tgatcagcgg caactgggcc aatggcggag gaggaatttt ctgcgacggc actcaagggg
    15541 gaaacagttc cccccggctg atcaattgca tcttgtccgg caacggtgcg aatgcgagcg
    15601 gtggagcgat gtttaatgat gggaataccg gcaccagttc accaagtctg gacaattgcg
    15661 tggtttccgg caatatcgcc tattatcagt gggggggagg gatgtacaat tcagggatca
    15721 attcaggagt atgcgctcct caaatcagga attccatctt ctggaacaat tttgcggata
    15781 tctctggtcc cgtcttccat aataatgatg ctgtaaccac ggtctcgaat tccatattac
    15841 aagggagcac tgacctgaac gatggttcgg tttcttccaa tgaaaacggg gggacaacca
    15901 ctggcagcga caacttattc agcgacccgc tatttattaa cgctccagct gcagccgttc
    15961 ccccaaccac agccggtgat ttcagggtag caagttattc ccctgcagtg aattttgggg
    16021 acgatgctgc cattatggcg gctttgtcgg gtcatgcatt tattgacctg aatggaaacc
    16081 cccggatagc aggagcaggt gtggacgccg gagttttcga ataccagggt acggattgca
    16141 ctccatactc cggcatcaat actctttatg tcgatgccca ggcagccggc agtgaaacgg
    16201 gagcagactg gcccaatgcc ttgcttactt taaaggaagc tattgaaatt gcgacctatt
    16261 gtaccgggat cgattccatc ctggtcgccg caggcactta ttatccttcc tgtccggaca
    16321 cggtctatga tccctgtacc ggtgccgtgc aaacggtttt ggcacccagc cggcaacaat
    16381 ccttcatcct gcctgatggt atcacgctgc ttgggggcta tccgaccggt ggcattggtg
    16441 aacgggatcc gcattgcaat ctcaccatcc tgagcgggga tatcgatatg aacggggaca
    16501 gtaccggcaa taccttcaat attgtcaaga ccattgaata ccaggatacc attgtattcg
    16561 atggattcat catcgaacag ggcaatgcca ttgccaacgg tgaggaaggg ggcgcctggt
    16621 gggacggcag ccgcacggat tcggccttgt tatgcatcag caataccatt ttcagggaaa
    16681 actatgccgt attgggcggc gccttttatg gagaaggcac cagggattta aaaatcctgg
    16741 cctctgaatt ttacgagaat actgcggcgg aacggggggg agccgtgtat ttccaaccgg
    16801 tcgacaatat gttcgactcc gcctttacca ggatggaaaa tgtcctcctt catcacaata
    16861 cggcatcgat gaacggaggg gccatcgcca tttcctttca tcccaatatt tccctggata
    16921 tcatccatgc caccttcagt gacaacagcc cggatgtttt gaataaagct accgttggac
    16981 cggcgcggat ccgcaacagc atcatctgga ataacataag tcccgtattc acgaatttta
    17041 atttgccgga tgttcaattc tcgatcgtac aggacggatt ttccggcacc gggaataaat
    17101 tcgaagaccc cttgttccgg cagcccggct ttgattaccg attgcaatac gcttctccag
    17161 ccatagacat gggggataat gtcttcagca tggaaaccac tgacctggat gatcatgcgc
    17221 gcattcagaa cggaaccacg gacatggggg cttatgaatt ccaggcaccg gtgtgctccg
    17281 gatccggtac ggcctatgtc gactcttcag cggaagccgg aggggatgga acctcctggg
    17341 agaccgcctt caatgatctg caacaggcgc tggtcctggc ctatttctgc caggtggata
    17401 cgattttggt cgcccagggg acttattacc caagtcgtcc ggattcggta tttgattgcc
    17461 gcggggccct ggatacggta tacgcggcaa accgggatat ctcgttcaat attccggatt
    17521 ccattacgct actgggagga tttcccaccg gtggaggaac cctggaggaa agggactggg
    17581 agtgcaataa aaccatcctt tgcggggaga tccagcagga cggcgatcat accaacaaca
    17641 gttatcacgt ggtcaccaca atcggtgtgg acagcatcac caccgtggac ggattctgta
    17701 tccaggacgg gtacgccgtt tacaccagtt ttgagaactt tccaactttc cgcaacgtcg
    17761 gaggcgggtg gctgaacata ggggtcaata tttttataca actggatgac atggccatcg
    17821 cagactatta ttccaacccc aggatactta attgcacctt ttacaataac aatggccttc
    17881 ttggagcggg cctcggcaac ctaggcttcc tcagtaacgc ttcccccctg atctacaatt
    17941 gtaattttaa ttcggacagt gcgatcctgg gaggagcgat tgccaatatc gggatcagcg
    18001 gagaaagttc tccatatatt gtaaatacta aaatccaggg gaacacaggg ttagccgccg
    18061 ggggtatctt aaatcttggg ggatcggtcc agttaatatt tggattgcag gggatccgga
    18121 cccaatccga ccaaccaata aaaatggata gtgacaacgc gcaggtactg aaaggactga
    18181 ttcaatccgg gtttttaggt caattgggat ctgttaatca aaacaggaat gcgggtccag
    18241 agggcgccca atatctgatt gatcccgagt atggggtaag tgcaccggtg atcatcaaca
    18301 gtttgatcac cgggaatgca gccgccgccg gcggagggat ggtcaatgcg gttctgaccc
    18361 tggcgaatga agaatttatc gggcttagcg gcgagagttc acccaggctg attaattgcg
    18421 atatcaccgg caacctcgca gagattggag ccggtatctt aaatactgat tttatcctgg
    18481 atttgaccaa tggcctatct cctgttgaag gcggcgggcg actcagaccc tgcattgaaa
    18541 acagtatcct gtggaataac gatgccggag gagatggaaa tgatatcgca aatatgttcg
    18601 atgaagtctc cgattcgatt aaacttcatc acagcatcac cggggataat tacctggatc
    18661 tgaatcacaa ctgcatagaa tgtgtggata ctcttctagg tggaaataaa ctggctaccg
    18721 atcccctgtt tgtggatgct cctcccggtg gagcggcagc cactaccttg ggcaatttcc
    18781 atctgcagga cctttctccc gccatcaata tgggagaaaa cagcgtcaac ccgctttcca
    18841 cggatctgga tggcctggcc cggatagtcc aggcggtgat cgatatcggc gcttacgaaa
    18901 aacatttgtt ctgcaccgat gatacccttt acgttggggt agacggctcc cttccacccg
    18961 tggaagtaat ccttgaagaa acactaccgg atggctatga attgattgtg aatgggcttg
    19021 cggatgaaga tggaatgatc gaactcaatt gttctgatac cggactcaac gaacttgtga
    19081 ttgaaattgt gcggggctgt attccggtgt tggtggatag ttgtcatatg aatattacga
    19141 tcctggacac ttttgaaaag aacctggtct gcaatgatct cgtcaacgta tcccttggcg
    19201 ccggttgcct gaaagtgctg gacccggatg atttcctaga aaatatcaga gggtgcaggg
    19261 gattgtacga catccaggtg atctaccccc tgggcacgca ccgttttccg ggagaagacc
    19321 aggtggacct gagccatatt ggttacacga tggtgtacaa tgtatatgaa gtggggacgg
    19381 gcaataagtg ctggggctat cttaaagtgg aggacaaaca gccaccttta ctgaactgtt
    19441 cggtggacac catcacctgt tatgaactgc cggacctgcc gctgctggcg gagggggccg
    19501 acagtt
//
LOCUS       DAOQXT010000092        19217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3215_length_19217_cov_5.534026, whole genome shotgun sequence.
ACCESSION   DAOQXT010000092 DAOQXT010000000
VERSION     DAOQXT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19217
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3215_length_19217_cov_5.534026"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..768
                     /locus_tag="PKM27_14530"
     CDS             238..768
                     /locus_tag="PKM27_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR08532.1"
                     /translation="MLEQCLKEDRKAQQALYDRYAPILLGICRRYIRSDEDAEDVMIE
                     GFFKIFRNLRQFDRRGSFEGWMKKIMVNEALMWLRKKRLPQSELDEKLPLIQEDTDLR
                     QFELEGSEIIPLLDQLPDGYRTVFNLYVMEELKHREIAVMLGISINTSKSQLILAKKK
                     LKQLILEKQTKTQNKS"
     gene            765..1103
                     /locus_tag="PKM27_14535"
     CDS             765..1103
                     /locus_tag="PKM27_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08533.1"
                     /translation="MTPNHIETEFREKSRSIRIQPSAGSWDELSKRLDRPSSRQPFRL
                     SGWMAMAASFAGILLVSSLFLFQPSRKTDPFNSLPPATTFEYSESDLSSSIYEQISTL
                     HEAYRKMGYQ"
     gene            complement(1142..2326)
                     /locus_tag="PKM27_14540"
     CDS             complement(1142..2326)
                     /locus_tag="PKM27_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08534.1"
                     /translation="MDTIRVGIAGHAGLFEIKLSKKIKDCREYLDRLPSPSTLVSYPF
                     LTSIERCPPVGLQPAYAVLLDGSGKTVGFFYFQVIYFKASQSIRFNQDQDFFCRLHKS
                     LKGMVANLVEFNTLVGGNLLLSGPYGCILDPVYVSKQGLIYQHVLEEMQGWLRSRGVE
                     TSVTLVKDFPERDKYFEESTYHSFEVEPNMILRLGPWDKFEDYLDSLLSKYRVRARKA
                     IKSGAALQKHELGEKEIAAMNGRLFDLYRCTATGADFNLLDLDPMYFLELKKNMGSCF
                     HLYTYSLEGQPLAFYTVIEDGEFLEAHYLGIDEVLNKKYQLYLNILLDLIRCTIQGRK
                     KSIRFSRTALEIKSSVGAIAHPLTCYIKHRKVINNTFVPYLVDFLNKKKEWVPRHPFK
                     QP"
     gene            2470..3690
                     /locus_tag="PKM27_14545"
     CDS             2470..3690
                     /locus_tag="PKM27_14545"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNR08535.1"
                     /translation="MNRSNARAGPATFSLLAKVLFALHFFNLSPAQSVRCETDNAIPI
                     LSGTPHPYYPLQPQIRSDKALPVVVHVVWNRQEENIPDAEIKRQIDRLNLEFNNSYTR
                     RKSVPAEFRDKTGNPEIRFCLALFDPSGQTHTGILRTQTPTAGIGSVRDGQGRYLIHY
                     DQAGGSSAWDTQRYINIWIGKMDGAYGRSTIGGTAFSPAEDGIVIDPGRWGTDFSRNL
                     LGRTLVHEMGHYLGLKHTWGTTIGECTEDDGISDTPVQDGPYFGCPEYPQRSCGNSNM
                     FMNFMDFTDDYCLDLFTAEQVKRMHSVLAAFRPGLMNSAAACPTGTSPVSLSGIQVQY
                     RTSKGLLVIQAKEVPDSPIDLLISNSLGQYVYRYRVEIIDHAEIPAKNWPPGIYFINL
                     RYNKERRTIKFFKP"
     gene            3692..4390
                     /locus_tag="PKM27_14550"
     CDS             3692..4390
                     /locus_tag="PKM27_14550"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HNR08536.1"
                     /translation="MNRSILMLPLMVMAITCTRKTQSAAAQGEKLIEMDKSPCFGLCP
                     VYRLTVYRDGLMKMDAKQNMKISGLHTLQLNKADIASLRSKLEKMGLEKYQEEYREPV
                     ADAPSTELRYYIDGKPKRIFTNFIFPAPLQKITEELDQWAMGENWAKWIDPRIKKEFI
                     LLLEEGVPLSNILQKFSEQDMMPVRRLDPATRQYWLVTCLVMPGEEKSLMEQLRKDAE
                     IREVQENRTLEERQ"
     gene            4481..5131
                     /locus_tag="PKM27_14555"
     CDS             4481..5131
                     /locus_tag="PKM27_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08537.1"
                     /translation="MSILSLICIGSFLFFGILGIQQYPGGSDSQPGSQAFQWLENYWC
                     DLYAPTTYNGETNSARPWAMLATGLLAVGFILAWLSVPSLFSLSRWRAQIMVLPGISG
                     MALTVFLFSPWHDQVINFAGALLGLSIVAICPLLYRQGYTKFSFWGGLSVFTGAITYI
                     VYQTGLMPGSLPLLQKIAMLIFTGWMVSMNVAVLRIKGIEKTGGSNPPAHTTSSDL"
     gene            5283..7970
                     /locus_tag="PKM27_14560"
     CDS             5283..7970
                     /locus_tag="PKM27_14560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08538.1"
                     /translation="MKKYIKLLLSHNTKSFIRPDLRPLFIIVLVFAASHLAFTQKGLY
                     QYEESRIKSLPYHQDPVDLNGKALELRQSLLPGQWSRDFTVPGITGSIFVSATDGTNL
                     YVGGQFKTAGGVEANGIAKWDGQKWSSLGVGPENGVSGDVPQVEAIAYLDGRLYIGGQ
                     FSKAGSKTVNGVAYWDGNQWNTLGPDSANGVRRIFIINNDTIIGRGAVWALFAHQQKI
                     YIGGHFQYAGDSLANGITAWDPATGIWESFDGGFRGSSDTDPVYAYSFAAKGSDLYAG
                     GIFSTAGGVPAKNIAKWDGTNWIAIGNAENPVNDLRFDHAGKLLAVGYFNTNDQKGTT
                     RIGKWDGSTWTPVAGPKGLGTYISQFQVYGDTIYAWGSFDTLGYTLASLARLEGGNWQ
                     LLGGLGRAANEFLPGNVFGVELINNKLYVCGSFTKAGDLFPVNVVEWDLQNSKWKLLD
                     DGGANQGIHDGNIDVLEPAGKGLYAGGSFSVAGSQYTRNIAQWNGNKWESLGTGYNNG
                     IRGTVLSILVDSNDIYVGGYFGIAGTSEAFHVAKWDGNSWSPIGIGVGGVAGAHVKAL
                     AKVGHYLYVGGYFKVVGDAENYALPANSIARFDLITKRWETLGQGIEYVFGIPGAVYD
                     MEVDGDKIYVAGEFNSVDDKFYQNFAVLTNNKWSGIGDNHEIGIEGSARCVKLIGDDL
                     YIGGILRLERMGESVGILKWDGKKWIAVGEPLTADGRTVYVNNIVPHQNGLIAGGFFQ
                     KAGNKTVNHIAFYDGMEWNELGSGVIPGGLKIAKWGNKLFAAGPFDYVTAEGPGAGIA
                     QFEFDASTPSKDIVLPGALPLIKAYPNPARDFFSIDLSGDFNRGTLELFDPGGRLVRV
                     YSLAGAPSRFLTKGLHPGIYTFRVRSGKQILGGNKLLVE"
     gene            complement(7999..10611)
                     /locus_tag="PKM27_14565"
     CDS             complement(7999..10611)
                     /locus_tag="PKM27_14565"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNR08539.1"
                     /translation="MKLFWSFICVFLLYPGLPVMGQNQPLTGRPAIQDTVPKPDSLDV
                     LISKDAPDADVVYSAQDSSYLDAKEKIFHLFGDAKVTYQDYSLKAARIRLDMDHDIAY
                     AEGVQDSAGKWVGLPHFSDGEQEFDAIRMRYNFRTRKGIIDEVVTKYTDVFIRGTTTK
                     FVGGDPSDTTQTDIVYNRNAILTTCNAEHPHFGIRSTKQKFIKGRLVVVGPSNLEIMG
                     VPTPLWLPFGFFPITDKRSAGLIIPRDYEYSPSLGFGLRNVGFYTPLGPHFDLSTQFD
                     IYTRGSWGIQLGSNYAKRYKFSGLLNLGYSDRRFESGEKGDLRIEKSYTIQWSHRQAS
                     QANPYQNFSASVNIQTNDYQQLNYNDANIVLNNSLSSNINYNRTFPGKPFSFSASMSH
                     SQNTRDKSVIVSLPNFNFQMQRIYPFKRKQKAGDEKWYEQITFNYNANAQSLIRTTDT
                     ALFSSQSLNNFINSFGVRQVASSDVNFRVLKYFNLAPSINYREIWNLQQVNITNRNGS
                     PDTLQRDTLKGFKAAHLFNTSLSLNTAIFGTAVFKKGFIRGLRHTIRPGISLNYTPEN
                     NPYTGFYETRTGRRYYSIFEGMTYNEVPTSGRQLGIGYSITHIFEAKYFSKKDSAEKK
                     FKLFDNVYMSGFYNFAADSFKWSPMSVSGATRIFGGLTNLSIGASFDFYREDDAGRRI
                     NELLVKTGGGLARLQDYDIGLSSNFSFQDVKNLINKWKGQPAQEPEPEPDNEDELPAP
                     QKKKRENPETFWDMFKGFYFNHNFALGNIIDPVTREEKFGVIAHTLSTQGNIELTKNW
                     AVRVGNIGYDFKSKDLTYPDFGVTRRLHCWQMNFNYQPVRGTYSFNIYANPGTFSFLK
                     LPYRRNRFDPQNDL"
     gene            10819..11886
                     /locus_tag="PKM27_14570"
     CDS             10819..11886
                     /locus_tag="PKM27_14570"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNR08540.1"
                     /translation="MSKETRIAILAIVAITILILGYKFIIGKNLLTRSQYFYVKYENI
                     DQLQVSNPVMINGYEVGAVNKIYLDPADNTTPIVILEVNKDIKIPKNSKAELISLGVM
                     GGKAVQILLSGKCDADCAQSGDYLIPSSRSLLGSFVGEGEIDQYLEKIKISANQLFDS
                     IAGESGKKEIREGVQNFEKTLSNLAHSTTLLNNLLSRNSGKLDKMIASLESVVANIQG
                     SNKEITSILKNLDEMSTGLNKADPSQSVKALNETLAESKVAIENLQTTLKNADAALGE
                     VQTLIKKASTGDGTVAKLLDDPKLYQNLSNTARQLELLLQDLRLNPKRYVNVSVFGKK
                     DKAYTLPSLDPAMPPDSLKKQ"
     gene            complement(11934..12404)
                     /locus_tag="PKM27_14575"
     CDS             complement(11934..12404)
                     /locus_tag="PKM27_14575"
                     /inference="COORDINATES: protein motif:HMM:NF018738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HNR08541.1"
                     /translation="MLGKVKLFFGIEGLKVDLEVPLAFKAEDKVIQGILKLHSKAPQT
                     IRSIEIKLVETYHRGRGQEKRTNDYVWGRIAWNESLEIPANGEKIIDFRLPVEPQLSS
                     IEKMAGKSAVHHKIASFARLLKNAKSVFHVEVSAKVEGTALNPGIKKEIKLISA"
     gene            complement(12394..12900)
                     /locus_tag="PKM27_14580"
     CDS             complement(12394..12900)
                     /locus_tag="PKM27_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08542.1"
                     /translation="MLIRFEDMPGHSRVWIFQAERTLGEGEEMMLDILIRNFLEDWTS
                     HQRTIRASYEIRHHRFVILAVDEDQVGASGCSIDKLFRFVQSLESELGFVLLDKQKIA
                     LQQAGEIVSVPLHTLKEKVRAGEMDKEEKYFDLMIAQKESLGEFLKPLSSGWLARHLS
                     NEFKDHAR"
     gene            complement(12915..13694)
                     /locus_tag="PKM27_14585"
     CDS             complement(12915..13694)
                     /locus_tag="PKM27_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNR08543.1"
                     /translation="MDRIKTVSEYISEGERPEILFWVGCAGSFDPRAQQVSRAFASLM
                     KIAGIRFAILGDEEKCTGDPARRAGNEFLFQLLALQNIETLKNYEITKIVTTCPHCFN
                     TLKNEYPALGGHYEVVHHTVFLNDLLRDGRLKLAGEIPGGALTYHDSCYLGRINGIYE
                     DPREIMKQMNADIREMKRSKAGGLCCGAGGGQMFKEDEAGSKRINLERVEEALSTGAG
                     TIVSNCPFCLTMLTDGVKALDQQEKVMVYDLAELILQQKSP"
     gene            complement(13701..15020)
                     /locus_tag="PKM27_14590"
     CDS             complement(13701..15020)
                     /locus_tag="PKM27_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNR08544.1"
                     /translation="MLSILLFTLLTLGGFSLAFLNFRKIYRNICLGKPEGRLDQRWTR
                     LKNMLLVAFGQKKMFSLWKPAVLHLAIYLAFLLTQIELIEIILDGFSHSHRVLAPLLG
                     GFYTFLISAIELLSLFALVATVIFLYRRNILRLSRFQKPEMKGWPSWDANLILIGEIL
                     LVLAIFTMNGTDRVLQQVDPQQYQETGFFLVSGWLGPLMFGDYSASSLHLLERGGWWL
                     HYLVVLGFINYLPISKHLHIFLAFPQTWYSKLKPRGEMENMPVVMREARSMLDLPPVP
                     EPASTENESPEFGAKDVFDLPRRVLLGAYSCTECGRCTAVCPANLTGKKLSPRKIMMD
                     IRDRSEEIGRNLDKGKFSVSGYQDGKSLFSYILPEEIHACTTCQACVEACPVLINPME
                     PILELRRHEILSQSAGPAQWTAMFNSLENNGSVWPMTDARSAWAEKQ"
     gene            15074..15565
                     /locus_tag="PKM27_14595"
     CDS             15074..15565
                     /locus_tag="PKM27_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNR08545.1"
                     /translation="MKILNESQIRQKIKRLAIQILENNTDSKEIILAGINNKGYGFAE
                     YLKSEIAGFSDLPVILTRIKLNPADPLSREITIDLTPTQLEKKTIIVVDDVANTGRTI
                     FYAMKPILETLPKKLEVAVLVNRMHKSFPIHVDYVGLTLSTTLEENIEVELGGKEWSV
                     VLS"
     gene            complement(15661..16143)
                     /locus_tag="PKM27_14600"
     CDS             complement(15661..16143)
                     /locus_tag="PKM27_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HNR08546.1"
                     /translation="MNNEWSGTDKHSEEFINPVDKTKVTSTPGSLPYPHHAGSALVRP
                     EDMGKVQGLALQAMYQQTDTQLQQIREQIELLLAQGKNIQTRIQVSEQIYQAAIGFDP
                     VINHIYHLYQKKSDQAWALSMVAPHEWGRKKPYEYLATVRLLADHTWEILKTRETDEG
                     "
     gene            complement(16145..17176)
                     /gene="ruvB"
                     /locus_tag="PKM27_14605"
     CDS             complement(16145..17176)
                     /gene="ruvB"
                     /locus_tag="PKM27_14605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNR08547.1"
                     /translation="MIHRPNDPLNPAPSPEEKQIEKTLRPAALEEFSGQSKIVDNLKV
                     FIQAARMRGEALDHVLLHGPPGLGKTTLAHIISNELGSSLKATSGPVIERPGDLAGLL
                     TNLNAGDVLFIDEIHRLNSMVEEYLYSAMEDYRIDIMIDSGPNSRSVQISLNPFTLVG
                     ATTRMGLLTAPLRARFGITCHLDYYDFHTLVGIVKRAAGILAIPVTRDGAEEIARRSR
                     GTPRIANALLRRVRDFAMVQGDGTINIEIAKHALMALNIDRCGLDEMDKRILNTIITK
                     FRGGPVGLNTISTAVGEEAGTLEEVYEPFLIMEGFIQRTPRGREATHKAYEHLGIAPP
                     AAGGPTLFD"
     gene            complement(17181..17774)
                     /locus_tag="PKM27_14610"
     CDS             complement(17181..17774)
                     /locus_tag="PKM27_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08548.1"
                     /translation="MIHLWQILFILPFLSGREASEPHLVKCKVDAKVLDYTALDGCRF
                     ILELKDGTRLMPVELSDKEFLFSEGQQVKITYTEAKGVVSTCMTSAVPVKIECIQQVK
                     EGTKPGQVFIKKECYDTEAPLNVAWLNRVILRNKVIDVKKGYENQLPYYVLYGNVNVM
                     LFNCTGGIECEYALTSLGSCSERIESLSNLKSVWSQK"
     gene            complement(17787..19019)
                     /locus_tag="PKM27_14615"
     CDS             complement(17787..19019)
                     /locus_tag="PKM27_14615"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNR08549.1"
                     /translation="MEKIKVYNPVAELDGDEMTRIIWKFIKDKLILPYLDLPIQYFDL
                     GIEHRDATNDQVTIDAAQAIKACGVGIKCATITPDEARVEEFKLKQMWKSPNGTIRNI
                     LDGTVFREPIVMQNVPRLVSNWTAPIIIGRHAFGDQYRATDFVTRGKGKLTITFSPED
                     GSAPVSHEVYHFQGDGVALAMYNTDESIRGFARACFNMAIQKQWPLYFSSKNTILKKY
                     DGRFKDLFQEVYEREFKSVMDGMNLVYEHRLIDDMVASALKWNGNFVWACKNYDGDVQ
                     SDTIAQGFGSLGLMTSVLITPDGQTMEAEAAHGTVTRHYRDHQAGKPTSTNPIASIFA
                     WTRGLEFRGKKDGNQPLIDFCHALEKVCIETVEKGKMTKDLAVCIHGNKVEHGKHYLY
                     TEEFLDAIDEGLKIKLNR"
     gene            complement(19031..>19217)
                     /locus_tag="PKM27_14620"
     CDS             complement(19031..>19217)
                     /locus_tag="PKM27_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08550.1"
                     /translation="KKQEAKVENGQPNSGMDRLPPTTHHPLLTTHYPPPTIPSLPPFL
                     PSPFLPCPKNGIFAPDL"
BASE COUNT         4707 a         4877 c         4921 g         4712 t
ORIGIN      
        1 tttgtgggac accatctttg tgggacactc gtgagaacca cgtaaagggt caacggtaaa
       61 cggtaaaagg ccaagggagc ataattactt taaaatcaac atgcttcgtg gtcttcgcgt
      121 ctttgtggga caccatcttt gtgagcacca aagccctgtg cgaacccacc agaaaccaca
      181 aaattcgcat ctttaataaa aaaagccatc ggatcatcag cggtacatac agaccagttg
      241 ctggaacaat gtcttaaaga agaccggaaa gcccaacagg ccttatacga ccggtacgcg
      301 cccatcctgt tgggaatttg ccgccgctat atccgcagcg atgaagacgc ggaagatgtg
      361 atgatcgaag gatttttcaa gatcttccgc aacctgaggc aattcgaccg gaggggaagt
      421 tttgagggct ggatgaaaaa aatcatggtc aacgaggccc tgatgtggct gcgtaaaaag
      481 aggctgcccc agtctgaact ggatgaaaaa cttcccctga tccaggaaga taccgacctg
      541 cggcagtttg aactggaagg ctccgagatc atccccctgc tggaccagct gccggatgga
      601 taccggaccg tattcaatct gtatgtcatg gaagagctca aacaccggga gattgcggtg
      661 atgctcggca tcagcatcaa tacctccaag tcgcaactga tcctggcaaa gaaaaaatta
      721 aaacaactga tattggaaaa acagacaaaa acacaaaaca aatcatgaca ccaaatcata
      781 tcgaaaccga attccgtgaa aaatccagat ctatccggat ccaaccttcg gccggctcct
      841 gggatgaact gtccaagcgc ctggatcgac cctccagccg tcaacccttc aggctgagtg
      901 gctggatggc catggcggcc agctttgcgg gaatcctcct ggtcagttcc ctgtttttat
      961 tccaaccttc ccggaaaaca gacccgttca attcccttcc ccccgccacc actttcgaat
     1021 attccgaatc cgatctgtct tcctccatct acgaacagat ttccacgctg catgaggcgt
     1081 accggaagat gggataccag taatcagtag tcagtagtca gtagtcagta taggctgtgg
     1141 atcaaggctg tttaaacgga tgtctcggca cccattcctt cttcttattc aaaaaatcca
     1201 ccaggtaagg cacgaaagtg ttgttgatca ctttccggtg tttgatataa caggtgaggg
     1261 gatgtgcaat cgcccccaca ctgcttttga tctccagggc ggtgcgtgaa aaccggatgg
     1321 atttcttccg gccttgtatg gtgcaccgga tcaggtcgag caatatgttg agatacaatt
     1381 ggtatttttt atttagcact tcatcgattc ccaggtagtg ggcttccagg aattccccat
     1441 cctcaatcac cgtataaaaa gctaggggct ggccctccag gctataggta taaagatgga
     1501 aacaggaacc catgtttttt ttcagttcca gaaaatacat gggatccaga tccaaaaggt
     1561 taaagtcggc cccggtagcc gtgcaccggt aaaggtcgaa gagacgcccg ttcatggcgg
     1621 caatttcctt ctcgccgagc tcgtgttttt gaagggcggc gccggatttt attgcctttc
     1681 tggcccggac cctgtatttg gacaacaggg aatccaggta atcttcaaat ttatcccagg
     1741 ggccaagtct caaaatcata ttgggctcca cctcaaaaga gtgataggta ctttcttcaa
     1801 aatatttatc cctttccgga aagtccttga ccagggtgac gctggtttca actccccggg
     1861 aacgcaacca gccctgcatt tcttccagga catgctgata gatgaggccc tgcttagaaa
     1921 catataccgg atccaggata caaccataag gaccgctgag caacaaattt ccccccacca
     1981 gggtgttgaa ttccaccagg ttggccacca tgccctttaa gcttttatgc aggcggcaga
     2041 aaaaatcctg atcctggttg aaccggatgc tttgggaagc cttgaaataa atgacctgga
     2101 aataaaagaa acccaccgtt ttcccggacc cgtccaggag aacggcatag gcgggttgca
     2161 agccgaccgg aggacaacgt tcgatggaag tcagaaaagg gtaggaaacc aatgtacttg
     2221 gagagggcaa tcgatccagg tattccctgc aatccttaat cttcttactt aatttaattt
     2281 caaacaaacc ggcatggccg gcaatcccta cccgaatcgt gtccatcagg tgcaaagata
     2341 aggcgggggg gggattaaga cagagggtgt cttcgagaca ccccctgtct gcgtgtcttc
     2401 gagacacccc ctgtctgcgt gtcttcggcc ccccctgact tactctcatc tccttatctt
     2461 tacctctcca tgaacagatc gaatgcaagg gccggcccgg ccaccttttc actcctggca
     2521 aaggtgctgt tcgcgctcca cttcttcaat ttatcccctg cccagtctgt tcgctgcgaa
     2581 accgacaatg ccatacccat tctgtcaggc accccccatc cgtattatcc tctacaacct
     2641 cagatcagat ccgacaaggc cctcccggtg gtcgttcatg tggtctggaa ccgccaggaa
     2701 gaaaacatac ctgatgctga aatcaagcga cagatcgacc ggctcaacct tgaattcaac
     2761 aattcataca cgagacgcaa aagcgtaccc gccgaattca gggacaagac aggaaatccg
     2821 gagatccggt tttgcctcgc cctcttcgat ccatccggcc aaacccatac gggcatcctt
     2881 cgtacccaga ccccgactgc agggatcggc tccgttcggg atggtcaagg gaggtacctc
     2941 attcattatg accaggcggg cggcagttca gcctgggata cccagcgtta catcaatata
     3001 tggattggaa agatggatgg cgcttatggg aggagcacga tcgggggaac ggcattcagt
     3061 ccagctgaag atggcatcgt catcgatccg ggccgctggg gcaccgattt cagccgtaac
     3121 ctgctcggga ggaccctcgt gcatgagatg ggccattacc tgggactgaa acatacctgg
     3181 ggcacgacga tcggagaatg tacggaggat gacggcataa gcgatacccc ggtccaggac
     3241 ggcccttatt ttggctgccc ggaataccct cagcgttcgt gcggcaacag caatatgttt
     3301 atgaacttta tggattttac ggacgattat tgcctcgatt tgtttacggc cgaacaggta
     3361 aaaagaatgc attccgtcct tgctgccttc cggcccggat tgatgaattc tgcggcggcc
     3421 tgccctaccg ggacctctcc ggtcagcctt tccggaatcc aggttcaata tcggacatca
     3481 aaaggcctcc tggtgatcca ggcaaaggag gtacccgatt caccgatcga cctgctcata
     3541 tccaacagcc tgggccagta tgtctatcga tatcgtgtgg aaattataga ccatgctgaa
     3601 atcccggcga aaaactggcc tccaggaatt tacttcatta atttgcgtta taataaggaa
     3661 aggaggacga tcaaattttt taaaccctga tatgaataga tccatactga tgcttccgtt
     3721 gatggtgatg gccattactt gtacccggaa aacacaatcc gcggccgccc agggtgagaa
     3781 attaatcgaa atggacaaat ccccctgttt tggcctatgt ccggtttacc gcctgaccgt
     3841 ttaccgggat ggcctcatga aaatggatgc caaacaaaat atgaaaatca gcggtctcca
     3901 taccctgcag ctcaacaaag ccgatatcgc ttcgcttcgc tcgaagcttg aaaaaatggg
     3961 attggaaaaa taccaggaag aataccggga gccggtggcg gatgcgcctt ccacggagtt
     4021 gcgatattat atagacggga agccaaaaag gatatttacc aattttatct tcccggcacc
     4081 ccttcaaaag atcacggagg aattggatca atgggcaatg ggggaaaact gggccaaatg
     4141 gatcgatccg agaataaaga aagaattcat cctcttgctg gaggagggag tgcccttgtc
     4201 aaacatcctg caaaaattca gtgaacagga catgatgccg gtacgacgcc tcgatcctgc
     4261 gacccgtcaa tactggctgg ttacctgcct ggtcatgccg ggggaagaga aatcactgat
     4321 ggaacaactg cgaaaagatg cggagatcag agaggtacag gaaaacagaa ctttggagga
     4381 aaggcaatag gcaggaggca ggagtcaata gtcaatagtc aagagtcaag agtcaagagt
     4441 caagagtcaa gagtcagtgg caggtataag ttccaggaaa ttgtccatcc tttccctgat
     4501 ttgcatcggg tcctttttgt tttttgggat cctgggaatt caacaatatc ccgggggaag
     4561 cgattcccaa cccggatcgc aggcctttca atggctggaa aattattggt gcgacctgta
     4621 tgcccccacg acttataatg gggaaacgaa ctcggcgcgt ccctgggcca tgcttgccac
     4681 cggtttgctg gctgttggtt ttattcttgc atggctaagc gttccatccc tgttttcctt
     4741 atcgcgttgg cgggcgcaga tcatggttct acccggcatt tccggaatgg cgcttacggt
     4801 tttcctcttt tccccctggc atgaccaggt cataaatttt gccggggcat tactgggctt
     4861 gtccattgtg gccatctgcc ctctcttgta tcggcaggga tacaccaaat tcagtttttg
     4921 gggaggctta agtgttttta caggggccat cacctatatc gtttaccaga ccggcctgat
     4981 gcccggttcc ctgcctcttc ttcaaaaaat agccatgttg atttttacag gatggatggt
     5041 gtccatgaat gtggcggtac tgagaataaa gggaattgaa aaaacaggag ggtccaatcc
     5101 tcctgctcat actacatctt ccgatttgta gatcacctgg aggattcaaa tcaccagtat
     5161 cgggtgcata tcaaactgca ggtctttttg cttgataaca agacctggaa ggtttcaact
     5221 cgcataaaaa cgaaattatc cggtcctaaa ccggggcact gatttttcat tactttgcaa
     5281 ctatgaagaa atacatcaaa ctgcttttat cccataatac caaatcgttt ataagaccgg
     5341 acctgaggcc cctttttatt attgtactgg ttttcgctgc aagccacctg gcctttacac
     5401 aaaaaggcct ttaccaatac gaggaaagcc ggattaaatc cctgccatac catcaggatc
     5461 cggttgactt aaacggtaag gccctggagt tgaggcaatc ccttttgcct gggcaatgga
     5521 gcagggattt taccgtacct gggattaccg gttccatttt cgtttccgcc accgacggca
     5581 ccaatttgta cgttgggggc cagtttaaaa cggccggggg ggttgaagcc aatggcattg
     5641 ctaaatggga cggccaaaaa tggtcttctt tgggagtggg gccggagaat ggggtatccg
     5701 gagatgtccc ccaggtggaa gccatcgcgt atctggatgg tagattgtat atcgggggcc
     5761 agttttccaa ggcgggatcc aaaaccgtga acggcgtggc ttattgggac ggaaaccagt
     5821 ggaataccct cggaccggac agcgccaatg gggtcagaag gatatttatt ataaataatg
     5881 acaccatcat cggaagggga gctgtctggg ccttgtttgc acaccagcag aaaatttata
     5941 taggaggcca cttccagtat gccggggaca gcctggccaa cggcatcacc gcctgggacc
     6001 cggccaccgg gatctgggaa tcatttgatg ggggattccg gggctcttcg gataccgatc
     6061 cggtctatgc ttatagtttt gcggccaagg gcagtgatct ttacgcggga ggaatattct
     6121 ccacggccgg gggagtgccg gcaaaaaata ttgccaaatg ggatggaaca aactggattg
     6181 ccatcggtaa cgccgaaaac cctgtcaacg acctgaggtt tgaccatgca gggaagctgc
     6241 ttgctgtggg ttattttaat accaatgatc aaaaaggaac gacccggatc gggaaatggg
     6301 acggcagcac ctggacgccg gtggccggac ccaaagggct tggcacctat atctcccagt
     6361 ttcaggttta cggagacacg atctacgcct ggggcagttt tgacaccctg ggctataccc
     6421 ttgcctccct tgcaaggctg gaagggggca actggcaact ccttggtgga ttgggacgcg
     6481 ccgccaatga attcttaccg ggaaacgttt tcggggtaga gctgatcaac aataaattgt
     6541 atgtctgcgg cagttttacg aaagcgggag accttttccc cgtaaacgtc gtcgaatggg
     6601 acttgcaaaa ttcaaaatgg aaattgctgg atgacggggg ggccaaccag gggatccacg
     6661 atggaaatat tgacgtcctg gagccggctg gaaaagggtt gtacgcagga ggcagttttt
     6721 ctgtcgcggg gagccaatac accaggaata tcgcccaatg gaacgggaat aaatgggaat
     6781 ccctcggcac cgggtacaac aatggcatcc ggggtactgt cctgagcatc ctagtggaca
     6841 gcaacgatat ttatgtaggc ggatattttg gaattgcagg cacctcggaa gcttttcatg
     6901 tggcgaagtg ggatggcaac agctggtccc ccataggcat tggcgtaggt ggcgttgcgg
     6961 gcgctcatgt aaaggccctg gccaaggtcg gacattacct ctatgtcggt ggttatttta
     7021 aagtggtagg cgatgctgaa aattatgctt tacccgcaaa ttccatcgcc cggtttgacc
     7081 tgatcaccaa gcgttgggaa accctgggcc agggcattga atatgtgttc ggaattcccg
     7141 gtgcggtcta tgatatggaa gtggacgggg acaagatcta tgtggccggc gaattcaaca
     7201 gcgtggatga caagttttac cagaatttcg cggtcctgac caataacaaa tggtcaggca
     7261 tcggagacaa ccatgaaatc ggcatcgagg gctccgccag atgcgtcaaa ttaatcggag
     7321 atgacctcta catcgggggc atcctccgtc ttgaaagaat gggcgaatcc gtggggatac
     7381 tgaaatggga cggtaaaaaa tggatagccg tcggcgaacc cctgaccgct gacgggagga
     7441 ccgtgtatgt aaacaatatt gtgccgcacc aaaacggcct gatcgccggc ggcttcttcc
     7501 aaaaagcagg aaataaaacc gtgaaccata tcgcgttcta cgatggcatg gaatggaatg
     7561 agctgggaag cggggttatt cccggtggcc ttaaaattgc caaatgggga aacaaattgt
     7621 ttgcggccgg gccctttgat tatgtcactg ctgaaggacc aggcgcaggc atcgctcaat
     7681 tcgaatttga tgcttccacg ccttcaaaag atatcgtgtt acctggcgct ttgcctttga
     7741 tcaaggcata tcccaacccg gcccgtgatt ttttcagcat cgacctgtcc ggtgacttca
     7801 accgcggtac tcttgagttg tttgatcccg gcggacgact ggtccgggtc tattcgctgg
     7861 ccggagctcc ctcccgtttt ttgaccaaag gacttcatcc gggaatttat acattccggg
     7921 tcaggtcggg gaagcagatt ctcggcggaa acaaactgtt ggttgaatag atcatggctg
     7981 atcactgata gctgatttct acaaatcatt ctgcggatca aaccggttcc tgcggtaagg
     8041 caattttaag aagctgaagg ttcccggatt ggcgtaaata ttaaaactat aagtgcctcg
     8101 taccggctgg tagttgaaat tcatttgcca gcaatgcagc ctcctggtga ccccgaaatc
     8161 cggataggtc aggtctttgg atttaaaatc ataaccaata tttcccaccc tgaccgccca
     8221 gttcttcgtg agctcgatat tgccctgggt gctgagggta tgggcgatga ccccaaattt
     8281 ttcttcccgg gtgacggggt cgatgatatt acccagggcg aagttgtgat tgaaataaaa
     8341 gcctttgaac atatcccaga aggtttcggg attctcccgt tttttcttct gtggtgccgg
     8401 caactcatct tcattgtcag gttcaggttc gggttcctgt gccggttggc ctttccactt
     8461 attgatcagg ttttttacat cctggaaact gaaattagaa ctcagcccga tgtcgtaatc
     8521 ctgcagccgg gccaggcccc ctccggtctt gaccagcaat tcattgatcc gccggcccgc
     8581 atcgtcttcc cggtagaaat cgaaggacgc accgatactc aaattggtca ggccgccgaa
     8641 tatccgtgtc gcgccgctga ccgacatggg tgaccacttg aaggaatcgg cagcaaaatt
     8701 gtaaaaaccg gacatgtaaa cattgtcaaa cagtttgaac ttcttttccg ccgagtcctt
     8761 cttactgaaa tacttggctt caaaaatatg ggtgatggag tatccgatgc ccagttgcct
     8821 gcccgaagta ggcacttcat tataggtcat gccttcaaaa atggaataat acctccgtcc
     8881 cgtccgggtt tcatagaagc cggtataagg attgttttcc ggcgtgtagt tcaatgaaat
     8941 gccgggccgg atggtgtggc gcagtccccg gataaatcct ttcttgaaca ccgccgtacc
     9001 gaatatggcc gtgttgaggc tgaggctggt attaaaaaga tgagccgctt tgaatccctt
     9061 cagggtatcc ctttgcaggg tgtccgggct gccgttccta ttcgtaatgt tcacctgctg
     9121 caggttccag atttcccggt aattgatgct cggggccagg ttgaaatatt tcagcaccct
     9181 gaaattgacg tcggaggagg ccacttgccg gacgccaaag ctgttgatga aattgttcag
     9241 ggactggctg gaaaaaaggg cggtatcggt cgtgcggatg agggactggg catttgcatt
     9301 gtaattaaaa gtgatctgtt cgtaccattt ttcatccccg gccttttgtt tccgcttaaa
     9361 gggatagatc cgctgcatct ggaaattaaa attgggcagg ctcacgatca ccgacttatc
     9421 cctggtgttt tgcgaatggc tcatggaagc ggaaaaggaa aaaggtttgc ccgggaaggt
     9481 ccggttgtag ttgatgttgg aggagaggct attgttcagg acgatgtttg catcattgta
     9541 attcaactgc tggtagtcgt tggtctggat attgaccgag gcggaaaagt tttggtaggg
     9601 attggcctgg gaagcctggc ggtggctcca ttggatcgta taggattttt cgatcctcag
     9661 gtctcctttt tccccggatt caaacctgcg gtccgaatac cccaggttga gcagcccgct
     9721 gaatttatac cttttggcat aattggatcc cagctggatc ccccaggatc ccctggtgta
     9781 gatatcaaac tgggtgctca ggtcaaaatg cgggcccagc ggggtataaa agcccacgtt
     9841 cctcaagcca aaacccaggc ttggactgta ttcataatcc ctgggaataa taagtccggc
     9901 actcctttta tccgtgatgg ggaaaaatcc aaaaggcagc cacagggggg tgggcacgcc
     9961 catgatctcc aggttggaag ggcccaccac caccagtcgt cctttgataa atttttgttt
    10021 ggtgcttcga atcccgaaat gggggtgttc ggcattgcag gtggtcagga tggcattgcg
    10081 gttgtacacg atgtcggtct gcgtggtatc cgaaggatcc ccccctacga atttggtggt
    10141 cgtgccgcgg ataaagacat cggtgtattt ggtgaccact tcatcaatga tccccttccg
    10201 ggtcctgaaa ttatagcgca tacgaatggc atcaaattcc tgctccccgt ccgaaaaatg
    10261 gggcaggccc acccatttcc cggcactgtc ctgaacccct tccgcatagg ctatgtcgtg
    10321 atccatgtcc agccggatcc gggccgcttt cagagaatag tcctggtagg tcactttggc
    10381 atcgccaaaa aggtgaaata ttttttcctt ggcatcgagg taggaactgt cctgggcgct
    10441 gtaaaccaca tcggcatccg gcgcgtcttt ggaaatcagg acatccaggg aatcgggctt
    10501 cggtaccgta tcctggatag ccggacgccc ggtcaacggc tggttttggc ccattaccgg
    10561 cagtccggga taaaggagga aaacgcaaat aaaagaccag aataacttca cgatcaacgg
    10621 ataaaacgaa ggataggccg gtattgtccc aaggttaacg atagaataat aagcttgtta
    10681 acatctgcaa aaataggaca ttgccgccag agcggactcc ttgcctttta agcctggcca
    10741 cctgtccctg ttccccgctt ctccagctcc cgaataccat gatagatttt tggtttttac
    10801 tgatatcttt gcgtttttat gtcaaaagaa acccgaattg ccatcctggc tattgttgcg
    10861 atcaccattc tcatccttgg ctataaattc atcataggga aaaacctgct gacccggagc
    10921 cagtattttt atgtcaaata cgagaacatc gatcaactcc aggtctccaa ccccgtcatg
    10981 atcaatgggt acgaggtggg ggcggtcaat aaaatttacc tggatccggc cgacaacacc
    11041 acgccgatcg tgatcctgga agtcaacaag gacatcaaaa tcccgaaaaa ttcgaaagcg
    11101 gaacttatca gcctgggagt tatgggaggc aaggccgtcc agattttatt gtccggcaaa
    11161 tgcgacgcgg attgcgccca aagcggggat tacctgatcc ccagctcccg cagcctcttg
    11221 ggcagttttg tcggagaagg tgaaatcgat caatacctgg aaaaaatcaa aataagcgcc
    11281 aatcaattgt ttgacagcat tgccggggaa tcgggaaaaa aagaaatccg ggaaggggtc
    11341 cagaattttg aaaaaaccct cagcaacctg gcccacagca ctaccctgct caacaacctc
    11401 ctttcgcgga actccggcaa actggataaa atgatcgcca gcctggaaag cgtggtcgcc
    11461 aatatccagg gctccaataa agaaatcacc agcatcctta agaacctgga cgaaatgagt
    11521 accggactga acaaggccga tccctcccag tcggtcaaag ccctgaatga aaccctggcc
    11581 gagagtaaag tggcgattga aaatcttcaa accacgctga aaaatgcgga tgccgcactg
    11641 ggcgaagttc agaccctcat caaaaaggca agtacgggtg acgggaccgt agccaaactc
    11701 ctggatgacc ccaaacttta ccagaatctg tccaacacag ccaggcaact ggaactgctg
    11761 ctccaggatc tccgcctgaa tcccaaacgc tatgtcaacg tctcggtatt tggcaaaaag
    11821 gacaaagcat acaccctgcc ctccctggat ccggcaatgc caccggattc tttaaaaaaa
    11881 caatagtctt ccccacgcgc ccggtccggg gtttcgatcc gggactccgg tcgtcaggca
    11941 ctgatcagct tgatctcctt cttgattccg ggattgaggg ccgtgccttc cactttggcg
    12001 gaaacttcga catgaaaaac ggattttgcg tttttaagca ggcgcgcgaa gctggcaatt
    12061 ttatgatgga cggcggattt gccggccatc ttctcaatgc ttgacaactg gggttccacc
    12121 ggcaaccgga aatcaatgat tttttcgccg ttggccggaa tttcaaggga ttcattccag
    12181 gcaatccttc cccagacata atcgttggtc cgcttttcct ggcccctgcc tcggtgatag
    12241 gtttccacga gttttatttc gatcgatcgg atggtttgcg gcgctttgga atggagtttt
    12301 aaaatgccct ggatcacttt gtcctccgcc ttgaatgcaa gggggacttc gagatccact
    12361 ttcaggcctt cgattccgaa aaacagtttt actttaccga gcatggtctt tgaattcatt
    12421 tgataaatgc ctggccagcc aaccggaaga aagtggtttc aggaactctc ccagactttc
    12481 tttttgggca atcatcaggt caaaatactt ttcttcctta tccatttccc ccgccctgac
    12541 tttctctttc agggtgtgca agggcacact gacaatttcc ccggcttgct gcagcgcgat
    12601 cttttgttta tccaacaaaa caaagcccag ttcagattcc aacgattgga cgaacctgaa
    12661 caatttatcg atcgaacacc cgctggctcc gacctggtct tcgtcgaccg caaggatcac
    12721 aaaccggtga tggcggattt catagctggc ccggatggtc cgttgatggc tggtccagtc
    12781 ctccaaaaag ttgcggatca ggatgtccag catcatttcc tccccttctc ccagggtcct
    12841 ttcagcctgg aatatccaaa ccctggaatg tcccggcata tcttcaaatc tgatcaacat
    12901 ggtatccccg caaattacgg gcttttttgc tgcagaatga gttccgccag gtcgtaaacc
    12961 atgacctttt cctgctggtc cagggctttc accccatccg tgagcatggt caggcaaaag
    13021 ggacaattgc tgacgatggt cccggcgccg gtggacaagg cctcttcaac cctttccaaa
    13081 ttgatccgtt tggatcccgc ttcatcctct ttaaacatct ggccaccgcc agccccgcaa
    13141 cagagtcctc cggcctttga acgtttcatt tcccggatat ccgcattcat ctgtttcatg
    13201 atttccctgg ggtcctcata aatcccgttt atccttccca ggtagcagga atcgtggtag
    13261 gtcagggctc ccccgggaat ttctccggcc agtttcaatc gaccatcccg gagcagatcg
    13321 ttcaggaaaa cggtatggtg tactacttcg taatgccctc ccagggcagg gtattcgttt
    13381 ttgagggtat taaaacaatg tggacaggtg gtcacgattt tggtgatttc atagtttttc
    13441 agggtttcaa tgttttgcag ggcaagcagc tgaaacagaa attcattgcc cgcccggcgt
    13501 gccgggtcac ccgtacattt ttcctcatcc cctaaaatgg caaaccggat gccggcgatc
    13561 ttcatcaggc tggcaaaagc acggcttacc tgttgtgcgc ggggatcaaa acttcccgca
    13621 catccgaccc agaaaaggat ctccggccgc tccccttccg aaatatattc cgaaaccgtt
    13681 tttatcctgt ccatatccat ctattgcttt tccgcccagg cagaccttgc atccgtcatg
    13741 ggccatacac ttccgttgtt ttcaagactg ttaaacatag ccgtccactg ggcaggccct
    13801 gcggactggc tcaggatttc atgccgcctt aactccagga tcggttccat cggattgatc
    13861 agcacagggc aggcttcgac gcaggcctgg caggtggtac aggcatgtat ttcttccggg
    13921 aggatatacg aaaagaggga cttcccgtcc tggtaaccgg aaaccgaaaa cttcccttta
    13981 tccaggttcc ggcctatctc ctcgctgcgg tcccggatat ccatcatgat cttgcgcggt
    14041 gaaagttttt tgcctgtgag gttggcaggg caaacagccg tacaccgccc gcattcggtg
    14101 caggaatagg cccccaataa aacccttcgc ggaagatcaa agacgtcttt ggcgccaaat
    14161 tccgggcttt cattttccgt ggaggcgggc tccgggacag gcggtaaatc aagcatcgaa
    14221 cgggcttcgc gcatcaccac aggcatgttt tccatttctc ccctgggctt gagttttgag
    14281 taccaggttt ggggaaaagc caggaaaata tgaaggtgct tggatattgg caggtaattg
    14341 ataaaaccga gcacgacgag gtaatggagc caccagcctc cgcgttccag caggtgaagg
    14401 gacgaagccg aataatcccc aaacatcagt gggccgagcc agcctgaaac caggaaaaaa
    14461 ccggtctcct ggtattgctg cggatccacc tgctgtaata cccggtccgt accgttcatg
    14521 gtaaaaatgg ccaggacaag caggatctcc ccaatcagga tgaggtttgc gtcccaactg
    14581 ggccagcctt tcatttccgg tttctgaaaa cggctgagtc tcaggatatt tctccggtac
    14641 aagaaaatga cggtagccac cagggcaaaa agcgataaca attcaatcgc ggaaattaaa
    14701 aaagtgtaaa atcccccaag caagggtgcg aggaccctgt gcgaatggct gaatccatct
    14761 aaaatgattt cgatcagttc gatctgagtg agtaaaaaag cgaggtagat cgccagatgc
    14821 agtaccgcag gcttccataa ggaaaacatt tttttttggc cgaaggcaac caggagcatg
    14881 tttttcagcc gggtccatct ttggtccaaa cggccttccg gtttacccag gcaaatatta
    14941 cggtagatct tcctgaaatt cagaaaggcc agggaaaatc ctcccagggt caacagggta
    15001 aataaaagga tcgaaagcat ccgggctaaa aattctaact tgcataaaat tacaaaatca
    15061 aggccccgca gtcatgaaaa tcctgaatga aagtcaaatc aggcaaaaaa tcaaacggct
    15121 cgccatccag atcctggaaa acaataccga cagcaaggaa atcatcctgg ccgggatcaa
    15181 caacaaagga tacggctttg cggaatacct caaatcagag atcgccggct tttcagacct
    15241 tccggtgatc cttacccgta taaaactgaa tccggccgac ccgctttccc gggaaatcac
    15301 cattgatctg acccctaccc agctcgaaaa aaaaaccatc atcgtcgtgg acgacgtagc
    15361 caataccggg cgtaccattt tttacgccat gaaacccatc ctggaaaccc tacccaaaaa
    15421 actggaagtc gccgtcctgg tcaaccgcat gcataaatcc tttcccattc atgtggatta
    15481 tgtcggcctc accctctcca ccactttgga agaaaatatt gaagtggaac tggggggtaa
    15541 ggaatggagt gtggtgttga gttaggttag agctgcgaga ttcgagattc gagattcgag
    15601 atacgagatt cgagatacga ggccattaac ccttgacctt ttacccttta cctttaacct
    15661 ttagccctcg tccgtttccc tcgtcttcaa aatttcccag gtatggtctg cgagcaggcg
    15721 gaccgtagcc aggtattcat aaggcttttt acggccccat tcatgcgggg ccaccatgga
    15781 caaggcccag gcctggtctg attttttttg gtaaagatgg tagatgtggt tgatcacggg
    15841 gtcaaagcca atggctgcct ggtaaatttg ttcggagacc tggatccggg tctggatgtt
    15901 tttaccctgg gcgagcagga gttctatttg ttccctgatc tgctgaagtt gggtatcggt
    15961 ctgctggtac atggcctgaa gcgccaagcc ctgaactttg cccatatctt ccgggcgcac
    16021 cagcgccgat ccggcatgat ggggataggg caggcttccc ggggtggagg tcaccttggt
    16081 cttgtcgacc ggattaataa attcttcgga atgcttgtcc gtccccgacc attcgttatt
    16141 catatcaatc aaataaagta ggtcccccgg cggccggtgg tgcgatccca agatgttcgt
    16201 aagctttatg cgtggcttcc ctgccccgtg gggttcgctg aataaatccc tccatgatga
    16261 ggaagggttc atagacttcc tcaagagtgc cggcttcttc acccaccgca gtggaaatgg
    16321 tattcaggcc caccgggcct cccctgaatt tggtgatgat ggtgttgagg attcttttgt
    16381 ccatctcatc gagtccgcaa cggtcgatgt tcagggccat gagggcgtgc ttggcgatct
    16441 caatattaat ggtaccatct ccctggacca tggcaaaatc ccgcacccgg cgcaacaagg
    16501 cattggcgat ccggggtgtt ccccggcttc tgcgtgcgat ctcttctgca ccatcccggg
    16561 taacggggat ggccaggatg cctgccgccc ttttcacgat cccaaccagg gtgtgaaaat
    16621 cataataatc gagatgacag gtgatgccaa aacgggcccg aaggggtgcc gtgagcaatc
    16681 ccatccgggt ggtggcgccg accagcgtaa acggattgag tgaaatctgg acactccttg
    16741 agtttgggcc ggaatcaatc atgatatcaa tccggtagtc ttccattgca ctgtacaggt
    16801 attcttccac catggaattc aggcggtgga tctcgtcgat aaacaggaca tcgccggcat
    16861 tcagattggt cagcaggccg gcaaggtctc ccggcctttc aatgaccggc ccggaggtag
    16921 ctttcaggct ggatccaagt tcatttgaaa tgatgtgggc cagggtggtc ttgcccaggc
    16981 cgggcggccc gtgaagcaat acatggtcca gggcctcgcc tctcattctg gcagcctgga
    17041 taaaaacctt gagattgtcg acaattttac tctgcccgct gaattcttcg agggcagccg
    17101 gccgaagggt cttttcgatc tgcttttcct ccgggctggg ggccggattc agggggtcgt
    17161 taggccggtg gatcatgcct ttatttttgc gaccagacgg atttaaggtt ggacaggctt
    17221 tcaatgcgtt cactgcagga cccaagggaa gtcagtgcat attcacattc gatccccccg
    17281 gtgcaattaa acagcatgac gttgacgttt ccatacagga cataataagg caactgattc
    17341 tcatatcctt tcttgacgtc aatcacctta ttgcggagga tgacgcgatt gagccaggcc
    17401 acattgagcg gggcctccgt gtcgtagcat tcctttttga taaaaacctg gcctggtttt
    17461 gtaccttcct ttacctgctg gatgcattcg attttaaccg gtacggcaga ggtcatgcag
    17521 gtggagacca cccccttggc ttccgtatag gtgattttaa cctgctggcc ctcgctgaat
    17581 aaaaattcct tgtccgacag ttccacaggc atcaaccggg tgccgtcttt gagttcaagg
    17641 atgaaccggc agccgtcgag ggcggtgtag tccaggactt tcgcgtcgac cttgcatttt
    17701 accaaatggg gttctgacgc ttcccttccg gaaaggaacg ggaggataaa aaggatttgc
    17761 cagagatgga tcataagatt gggttatcag cgattcaatt ttattttcag gccttcgtcg
    17821 atggcatcca ggaattcctc ggtatacagg tagtgttttc catgctccac cttgttgccg
    17881 tgaatgcaca cggccaggtc cttggtcatc ttaccctttt ccacggtttc aatacagacc
    17941 ttttcgagcg catggcaaaa atcgatcagg ggctggttgc cgtctttttt acccctgaat
    18001 tccaggccac gggtccatgc aaagatagaa gcaatcgggt tagtgcttgt gggttttcca
    18061 gcctggtggt cccggtaatg ccgggttacc gttccatggg cggcttccgc ttccatggtc
    18121 tgcccgtcgg gggtgatcag cacactggtc atcaatccga gggatccaaa gccctgggct
    18181 atcgtatcgc tctgcacgtc gccatcgtaa tttttgcagg cccagacaaa gttgccgttc
    18241 cacttaaggg cagaggccac catatcatcg atcaggcggt gttcgtatac caggttcatg
    18301 ccatccatga cggatttgaa ttcccgctcg tacacttcct gaaacaggtc tttgaaccgg
    18361 ccgtcgtatt ttttaagaat ggtatttttt gatgaaaaat aaaggggcca ttgtttctga
    18421 atggccatat tgaagcaggc gcgggcgaaa ccacggatgc tctcatcggt attgtacatg
    18481 gccagagcta cgccatctcc ctgaaaatgg tacacttcat gactgaccgg ggcgcttccg
    18541 tcttcaggac tgaaggtgat ggtgagtttt cccttgcccc gggtcacaaa atccgtcgcc
    18601 cggtactggt ctccgaaggc gtgacggcca atgatgatgg gagctgtcca gttgctcacc
    18661 agccggggaa cgttctgcat gacgatcggt tccctgaaca ccgtgccgtc caggatattg
    18721 cggatggttc cgttgggact tttccacatt tgttttaatt taaattcctc cacccttgcc
    18781 tcatccgggg tgatggtggc gcattttatc cctaccccgc aagccttaat ggcctgagcg
    18841 gcgtcaatgg tcacctggtc attggtggca tcgcgatgct cgatccccag atcaaaatac
    18901 tggatcggca ggtccaggta gggcaggatc agtttgtcct tgataaattt ccagattata
    18961 cgggtcatct catctccatc cagttcagcc accgggttgt acactttaat cttttccatc
    19021 tttgttttct ttataaatca ggcgcaaaaa taccattctt tgggcaaggg agaaaaggag
    19081 acgggagaaa aggaggaagg gatggaatgg tgggtggtgg atagtgggtg gtgagtagtg
    19141 ggtggtgggt agtgggtggt agcctgtcca tgcctgaatt tggttgaccg ttttcaactt
    19201 tagcctcctg ctttttc
//
LOCUS       DAOQXT010000093        19204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3219_length_19204_cov_6.812784, whole genome shotgun sequence.
ACCESSION   DAOQXT010000093 DAOQXT010000000
VERSION     DAOQXT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19204
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_19204_cov_6.812784"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKM27_14625"
     CDS             <1..242
                     /locus_tag="PKM27_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HNR08551.1"
                     /translation="NINSYQMKSAFGDHEKIPGKILLHASTPDDIRQALIKKGYTLEF
                     SARTSGPITAIYFDRLHGTMWGGASNHGDDYGVGW"
     gene            433..846
                     /locus_tag="PKM27_14630"
     CDS             433..846
                     /locus_tag="PKM27_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNR08552.1"
                     /translation="MYPLELVKPMENELLQAGFEALTDEQEVEQALSDKEGTVLLMVN
                     SVCGCAAANARPGVRLALKSGKTPDKLYTVFAGVDREATARAREFLAPYPPSSPSIAL
                     FKNGELVHMIERYQIEGRSADIVAANLKMAFDSYC"
     gene            1129..2823
                     /locus_tag="PKM27_14635"
     CDS             1129..2823
                     /locus_tag="PKM27_14635"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNR08553.1"
                     /translation="MLKSLPFLIFIFIASTVFSQGFIVEEFNATIRIEKSGYIQVHEH
                     LKVDFTERKRGIIRDIPFKYTYQGKSYRTPIDRVQVDQWDYKVSSQGSAKSIRIGHPE
                     KYIEGRQEYHIRYRVKGPFIHAAGYDEFYWNITGNGWTAPILKVHFDIEMPEELPFRY
                     QDLKVFTGRAGSRVDSAYIQQEGRHVKGSSIDVFNPGEGLTIALKLPANYIDREQTLD
                     MESTPSEKAARAFQKQWPPAVIPALLISLLVGFWNKLRGVNPPSQVIPQPYPPLDLNP
                     AEVGAFYDHVVNDRDVISLLPYWASQGHIRMEFDPDDQDTYLYRLNYLGGDSTGYEQV
                     LFSDLFSHRDVVRLSDLKYKFASTHQKVKSMIHREIIGMQLYDEKYRYWFSSWRTVLL
                     VAAFVPLGILFLVLGYWMAAVIFMIGLLTAIILVSQAKALSEKGKYFHHQLKGFYAFL
                     KNDQNQSYGEILEKDPLYFDKVYPYAVALKLDQIFIKKIRPYREFAPVWYGYYGMMGA
                     GRKSTMEDFGRDFQPKEISSAFSTVQPVSTGGGGGGGFGGGGSSGGGFGGGGGSSW"
     gene            complement(2942..3346)
                     /locus_tag="PKM27_14640"
     CDS             complement(2942..3346)
                     /locus_tag="PKM27_14640"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNR08554.1"
                     /translation="MKQLTHKLPVLALIFAVPVFCEFSFQPKDTWTWPTEPKNLKVLP
                     KGTAGDDLKAIMQSWNKALGVKCNHCHVSKEGAPFSQWDFVADTKPEKNMARDMVKMT
                     NGINKKYMTKKFQSKNMISCATCHRGEKAPSM"
     gene            complement(3518..4168)
                     /gene="pdxH"
                     /locus_tag="PKM27_14645"
     CDS             complement(3518..4168)
                     /gene="pdxH"
                     /locus_tag="PKM27_14645"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNR08555.1"
                     /translation="MKHSPLAHLRKSYSKSSLSELTAAVNPFDQFKSWFEESLKSHPA
                     EPNAMVLSTVGETGRPSSRIVLLKYYDASMGFLFFTNYESRKGRDLKSNPQASLLFYW
                     PEQERQIRVEGKVKKVSRSLSEEYFHSRPRGSQIGAWTSDQSQRVPNREYIDRINQEL
                     EKFFKEYEPVPLPPFWGGYSLVPDYFEFWQGREDRLHDRIAYRLSRKKWSVERLSP"
     gene            complement(4177..7581)
                     /locus_tag="PKM27_14650"
     CDS             complement(4177..7581)
                     /locus_tag="PKM27_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR08556.1"
                     /translation="MKDLIKEFKPSSWAIDNKTSIYILTIIITIAGIFSYIRLPKEQF
                     PEVAFPQILVNTVYPGTSPTDMENLVAKPIENQLKGISGVKKIRSSSLQDFSLVVAEF
                     GTDVDVAEAKLKVKDAVDRARADLPNDLPQDPSVIDIDVSSIPIMNIHVSGDYDLDRI
                     KKYAEQLKDEIETLKEITRADLVGALVREIQVNVDMYKMTAANVTMRDIETAIAMENM
                     TISGGQLDMGNVKRAISVVGEYKDARQLGDIIVRGGTGAVVYLKDIAEIKDTFMEQES
                     YARLNKKNVLTLNVIKRSGENLIETSDKIKAISERMQASAFPSDLNVNITGDMSLKTR
                     AGLKELINTIVIGFLLVTFILMFFMGATNAVFVALSVPLSMFVAFMVMPTYGFTLNVI
                     VLFGFLLALGIVVDDAIVVIENTHRIFDNGKVPIKRAAKMAAGEVFLPVLSGTLTSLA
                     PFIPLAFWPGLIGNFMFGLPITLITTLLASLLVAYIINPVFAVDFMQKHEEGKKEKIF
                     NRKFIVTLVFFGVVLLLSHLGQNYFIRNLALCIFLFYLLNRFVLNGLIRGFQTSVWPA
                     VQNAYASMLRVAVKGYRPIGLLLFTVFLLFVSLFLAFKYIPGVRLFPESDPNFVYTYI
                     TLPVGTDQAYTNAVTAEVENRIIGVVGEDNPIVESVISNVAVGAGDPSNFEQQTSPHL
                     GKVTVAFVEFEKRHGKSTVEYLNKIREVVKGIPGAEIIVEKENSGPPAGKPISIEIVG
                     EEFDELNQTAQNLKRYLDSLQIDGVEELKSDLSISKPQLKITVDRQRANTEGISTAQI
                     GSDLRSAVFGKEVSKLRDGEDQHPIQLRFKEDQRNNVNLLLNQSITYRDMNMGGQMRQ
                     VPMAALTQLEYSNTVGSIKRKNLKRLVTISSNVLAGYNPNEVLAKVQSATARFQAPPG
                     IEIGFAGEQEDQAEAMGFLGRSLLISLGLIFLILVTQFNSFSKPIIILVEIPFSIIGV
                     LLGFTMIRMEFSIIMTGIGIVALAGVVVRNGILLVEFAEMMMKQGYSIREAVVEAGRT
                     RMTPVILTAMATILGLIPLAIGFNIDFVTLFSEWSPNIYLGGDNVAFWGPLSWTMIFG
                     LGFATLLTLVLVPALLVIVSRLKARLGLEDYEAKALAEAAEESGQA"
     gene            complement(7606..8751)
                     /locus_tag="PKM27_14655"
     CDS             complement(7606..8751)
                     /locus_tag="PKM27_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR08557.1"
                     /translation="MKKITALVSGSLFLAILGTSCSSKSGSSPTPDKQSRLTELKNQI
                     ASLEKEAAALELELYPEGKVEKTKNVGIRVFPQTLFRNYIDLQGSVLADQELYINSRM
                     AGNAQNILIKTGDHIQAGQTVLTLDDALLQQGLAELEQQLQFATDLYQKQKALWDQNI
                     GTEVQYLSAKNNKESLEKRIATTKEQWDMTRVKAPISGVIDEVMVKPGQMVMPGAPIA
                     RLVNFSKLKLSVNVPESYAGRIKPGNAIKIWFPDIQKEYDSRITYVSPVINRVNRTFS
                     AEAALPPNMSGVMPNMIAIIKIVDYSNPNAYVLPINLLQKDSQGDYVMVADSTDGYWH
                     SSKRPVTIGKFYNDLVEIKSGIDASHKVITLGYQDLLEGERLSIQTY"
     gene            complement(8781..10172)
                     /locus_tag="PKM27_14660"
     CDS             complement(8781..10172)
                     /locus_tag="PKM27_14660"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR08558.1"
                     /translation="MKTKLLTSLFFLLLCTAGRPLMGQNSFSLEEAVQYALKNSAQVK
                     QNQLNLVDADAQLMEYKSIGIPKVNATIDFSHYFAIPTTILPDFLSPTIDNRLVHYNL
                     LDSNLVEDPKGGGIPARFGLSNSLSGALAMNTILYDPSFFVGLKATKLYKELVTRQNS
                     VSDITIRQSVAKAYLGALIAIRSKSILDQNIATLDKIVKETEAVRKQGFVEQLDVDRL
                     SLSLQTMQLEAEKANRMTEITLNVLRMHMGFPFGETILLTENLDVLADRLKIDNADLV
                     SAFRPEQRPEYRSMQMAEELSKIRMQTIKLSAYPVITGFANVSYGSQSNNLIDKDQTQ
                     WFPSSIAGFKLNLPIFDGFNRKARYQRANVELDKLRLQMNEFERAMNLEVSTARIQFD
                     NARTTLDGSQRNLDLAQKIYDQAQLKYKGGIGSSLEINQAETVFLQAQSGYIQALYNM
                     LVTKTDLDKALGK"
     gene            complement(10169..10789)
                     /locus_tag="PKM27_14665"
     CDS             complement(10169..10789)
                     /locus_tag="PKM27_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR08559.1"
                     /translation="MDLSSEILIRAKSLFIRCGIKSTTMDDLAKGLGISKKTLYEKVP
                     NKAALIEKMVHLHIREEKKALALIRSQAANAVEEMILIAQHVILMLRQVSPNTAYEIR
                     KYYWESWKKLDKLNQEHILGVIRQNLKDGIAQGYYRSELGVDIIAKNYVTLSAALIDE
                     TIFPAHLFSPEKVFKETIRYHLGGITTPKGWELCEHYIKKLDNPIL"
     gene            11056..11421
                     /locus_tag="PKM27_14670"
     CDS             11056..11421
                     /locus_tag="PKM27_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNR08560.1"
                     /translation="MKLQSKTILLLLAGSIYVMACQGKKSDSQDTAAAPAEPAAAAPA
                     SNAEGKKIFETYCVLCHGVDGKLGLNGSKDLSISVLPIEEKIIQVTNGKNLMTPFKDI
                     LTEDEIKAVVAYTEELKVK"
     gene            11421..12539
                     /locus_tag="PKM27_14675"
     CDS             11421..12539
                     /locus_tag="PKM27_14675"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNR08561.1"
                     /translation="MPSKAYRVLGLLSGSSLDGTDVAICKFVIDRDWPGLVREWHITE
                     AATIPYPDEWITRLKSLPSSTSRDLVIADTELGHYYGLISRDFIQRHGLPIDLIASHG
                     HTIFHFPELKTTTQIGDGAAITAETGIDSVTQLRGMDVAFGAQGAPLAPLGDKYLYPG
                     YSFYLNLGGIANLTTSVEGAYLAFDICPCNQILNALAGEAGLTYDHDGGLAASGKLIP
                     DLLQLYQSDPYLTRSYPKSLDNTWVVENHVNPSLGFNAPTADKLHTAVEFIALEIAKA
                     CALFPLPPNAGRPSMFVTGGGALNHYLVTRIQALAGELEVVLPSRTIIEFKEVSFIAL
                     AGLFRGLRIPNLFASVTGAPGDTINGALYTNQTTSPWS"
     gene            12536..13513
                     /locus_tag="PKM27_14680"
     CDS             12536..13513
                     /locus_tag="PKM27_14680"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR08562.1"
                     /translation="MNKKVLVTGATGFIGNYTVRHLLASGYEVSALVRPESLTLDSVD
                     SFRTQARIFEGDILDPDSLEVAFRDQDAVVHAAALVSFRPEDKSQLARINIEGTANVV
                     NTCLEQHIPHLVHISSIAALGGQPGQEWFDETSIWVKNKRHTTYAETKYQAELEVWRG
                     KAEGLNVCILNPSLVIGPWLPGHHSMKIFSHNLPFYPSGGNGLVDVRDVAEAVRICLD
                     RKVKDERIILNGHNLSYKEMLDRLATHKGKKGPAIALPRSLAMAGVQVLGLFNRIFRR
                     NTVFQPRLLHSVFSKAGFDNTKSVELLGLKYRPVDESIIAVLQGSRVNG"
     gene            complement(13563..14117)
                     /gene="lptC"
                     /locus_tag="PKM27_14685"
     CDS             complement(13563..14117)
                     /gene="lptC"
                     /locus_tag="PKM27_14685"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNR08563.1"
                     /translation="MLRYLGSLAAILFLGACENDFNLLQEAHQKKGNEEMVRDVVLHY
                     TDSGRLEVLLKAPVIKRTLQNNIPKQEFTEGIYCEFYDDSLRLKARLTARYAIRDENK
                     RIMTARDQVVLVSADSTRLESEELIWDEGRAIVYSNKFVKWSRGKEVGTGFFFQADQS
                     FKKIQMRKTEADNIVIPGLGRDEE"
     gene            complement(14102..>15718)
                     /locus_tag="PKM27_14690"
     CDS             complement(14102..>15718)
                     /locus_tag="PKM27_14690"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR08564.1"
                     /translation="NNAGTFQLCVDNYNPPVSTNGDCVTGSVLCSKDAFHVESVVGAG
                     LDRDEADNSCLDFNNSIPNNNNSESNSTWYRWTAANNGPLTFKITPDVPNDDIDFAVF
                     EFPNGLNNCANKRMVRCMATACVGPTGLNNTAQDTEENPDCNPGEDGFLKQLDMVKGM
                     SYGVLINNFTSTNHGFFIEFGGLGEFLGPETLIRVEPESGLKCEQSFTISDSTFFSAG
                     NIVKYTWNFGDGSLPSEVNFTKGPHEVRYKSFGLKTIVLVVESDKGCTYSFSKEINVQ
                     PCCEDLESLQINALAMDMVCHGVADGSVTISGMGGTPDYQFSINNEKFRRQSIYKGLN
                     KGSYLVRITDARGCQDTATVTIDEPPPVTVDVGPDLNGRLGYEVVLNGTYMAPDPIRS
                     IRWVPPDGIVCDSCLNTEVIPPGTTGYKLVVTTIKGCMGADSLIIRVTLDRSLYTPNV
                     FTPDQDSNNRIFRLYGNRAISSIEYLRVFNRWGGLVYEGRNLDINDESTGMGWHLQGR
                     SPTPRGIYLCGLRIVCRPDHPDNQRRCYVT"
     assembly_gap    15719..15818
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16082..16798
                     /locus_tag="PKM27_14695"
     CDS             16082..16798
                     /locus_tag="PKM27_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNR08565.1"
                     /translation="MVRKFIPNLFTLFNLLMGCISLYFVFTQQFRIVFWLILLAALAD
                     VLDGMVARWLKVDSGLGEQLDSLADVVSFGVVPGSILFQLIRYAWESNHMISTGMNGL
                     WLAFGGFIFTLGAALRLAIFNIDERQTTEFIGLATPGASVVVLGLMLVHVKGAGFMFD
                     LLSNEYVLLGFSILLFLLMLAPLRMFSLKSITKDWKANGLPIAFVLLAIPTALVFGGP
                     ALVLLPLLYVLLSVYLFRNT"
     gene            16795..17049
                     /gene="purS"
                     /locus_tag="PKM27_14700"
     CDS             16795..17049
                     /gene="purS"
                     /locus_tag="PKM27_14700"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNR08566.1"
                     /translation="MKFSAQIRVMPHKVLLDPQGKAVGNNLKRIGLEACTNVRVGKNI
                     QMEVEAPDQDSAEKLVRSACEKLLTNPIMEYFEYSLQAQN"
     gene            17049..17714
                     /gene="rsmI"
                     /locus_tag="PKM27_14705"
     CDS             17049..17714
                     /gene="rsmI"
                     /locus_tag="PKM27_14705"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNR08567.1"
                     /translation="MLYLVPTPIGDLEDITYRALRVLRSVDYILSEDTRTTQFLLQHY
                     QISKETRSFHDFNEHGRLPAVLADLKSGKNIALVSEAGMPGISDPGFLLVRACRQEDL
                     PLTVLPGPCALINALVGSGIPTEPFHFEGFLPHKKGRQTRLKYLSGLDCTFILYESPH
                     RILRCLEELITHCGPERPSAVCRELTKKFEEVRTANLGDQKKYFENEGVVKGEFVVVV
                     SGR"
     gene            17835..19175
                     /locus_tag="PKM27_14710"
     CDS             17835..19175
                     /locus_tag="PKM27_14710"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNR08568.1"
                     /translation="MIRRTVYGCLVLIVAATMLVSCKKDNPNYLVEVSDPSFYHRTMK
                     EVTDIMVYDIFSPPVASRIYSRVAIAGYEAMVPGDASYLSLAGQLNAMEAMPKPEAGK
                     EYAFPISSIKAMINVGRTLLFSEDKMDAYEQEILSEYRKIGVPEAVLARSLAFGDSIS
                     AAVLKWTSTDNYKQSRTFPKFSVSNEAVRWKPTPPAYMDAVEPHWNKQRPLCLDSCSQ
                     FKPKPPVPFSRDQDSEFYKIAKEVMDVVNRQDTTFNSIASFWDCNPFVMHQHGHVMFA
                     TKKISPGGHWIGVAGQMCRQLNKSFSETAEVYALLSIGLYDGFIICWDEKFRSNLVRP
                     ETYINENIDPEWTPLLQTPPFPEHTSGHSVVSSVSAEILSNLLGENIAFTDSVEIEYG
                     LFARSFPSFRKAAEEACISRFYGGIHYMPAITEGATQGINLGKYILERLKTRVE"
BASE COUNT         4423 a         4756 c         5116 g         4809 t
ORIGIN      
        1 ccaatatcaa cagctaccag atgaaaagcg ctttcgggga ccatgagaaa attccgggca
       61 aaatcctgct gcatgcctcc acgcccgatg acatacgaca ggccctgatc aaaaaagggt
      121 acaccctgga attcagtgcc cgcacctccg gcccgatcac cgcgatttat tttgaccgtc
      181 tgcacggcac catgtgggga ggcgccagca atcacgggga tgattacggg gtggggtggt
      241 agtagtggtt gaattgttcg gcatgggaat tggtaagtaa ttaagaatta agtaattaag
      301 aattaaggag taccacccac cacccaccac ccaccttttt tcccatttca ttacctagca
      361 ggcaggactt caccgtattt ttgtcctatc gtaaactatt tgttgtttcg gacgtttatt
      421 tgaaaaaaag aaatgtatcc tttagaactg gttaaaccga tggaaaatga gttgcttcag
      481 gcagggtttg aagccctgac tgacgagcag gaagtggagc aggcgctgag tgataaagag
      541 gggacggtat tgctgatggt gaacagcgtt tgcggctgcg cggcagccaa tgcccggccg
      601 ggtgtccgcc tggccctgaa atcaggcaag actcccgata aattatatac tgtatttgct
      661 ggagtggata gggaagctac cgcaagagcg cgggaatttt tagctcctta ccccccatcc
      721 tctccttcta ttgcattatt taaaaacggg gaattggtcc acatgatcga acgctaccag
      781 atcgaaggca ggagtgccga catcgtcgcc gccaatctca aaatggcttt cgacagctat
      841 tgctgatctt tgtacccgct ttagaccacc ttaaagaatt tacctccaaa ggttccattt
      901 taccggatca ggttccggtt ttttcatttt atgagttcct ttccatgggt taggaggtta
      961 cgttactgtc tcgcaatttt cccaccaggt cacaaaggat atatgatatt gattatcaat
     1021 aaaataaaaa ttgaatcttc gtgaacttcg tgtcttagtg ggaaccccac tctttgcggg
     1081 aaaagttttg gcccggaact ccgaatcttt tcttctcgtt aaaagggtat gttaaaatca
     1141 ctcccgtttc tcatcttcat ttttattgcc tccaccgttt tttcccaggg ctttattgtc
     1201 gaagaattca acgccacgat taggatcgaa aaatcgggct atatccaggt ccacgaacac
     1261 ctcaaagtgg attttacaga aagaaaaaga ggaatcatcc gtgatattcc tttcaaatac
     1321 acttatcagg gcaagtctta ccgcaccccc atagaccggg tgcaggtgga ccagtgggac
     1381 tataaggttt cctcccaggg cagtgccaaa tccatccgga taggacatcc ggaaaaatat
     1441 atcgaaggca ggcaggaata ccacatccgc tacagggtaa aagggccttt tatccatgcg
     1501 gcagggtatg atgaatttta ctggaacatc accggcaatg gctggacggc accgatccta
     1561 aaagtccatt ttgacatcga aatgccggag gaactcccat tccgttacca ggaccttaaa
     1621 gtattcacgg gcagggcggg gtcccgggtc gacagcgcct atattcagca ggaaggaagg
     1681 catgtcaaag gcagttcgat cgatgttttt aatccggggg aaggcttgac cattgcctta
     1741 aaattgccgg ctaattatat agaccgcgag cagaccctgg acatggagag cacaccttcg
     1801 gaaaaagcgg cccgggcatt tcagaaacag tggccccctg cggtcattcc cgccttactc
     1861 atttctttat tggtcggttt ctggaataaa ttacgggggg tcaacccgcc cagccaggtg
     1921 atcccgcaac cctatccgcc cctcgatctg aatccggcag aagttggcgc cttttacgac
     1981 cacgtggtca atgaccgcga tgtgatttca ttgctgccct attgggcatc ccaggggcat
     2041 atccggatgg aatttgaccc tgacgaccag gacacctatt tataccgctt aaattacctg
     2101 ggaggggaca gcacaggata cgaacaggtc ttgttcagtg atttgttcag ccaccgcgac
     2161 gtggtgcgtc tttccgacct gaaatacaaa tttgccagca cgcatcagaa ggtcaagtcc
     2221 atgattcacc gcgaaatcat cggtatgcaa ctctatgatg agaaataccg gtattggttc
     2281 agttcctggc gcaccgtcct cctggtggcg gcctttgtgc ccctgggcat ccttttcctg
     2341 gtattgggct actggatggc tgcagtgatt tttatgatcg ggctgctcac ggcgatcatc
     2401 ctggtcagcc aggccaaggc gctgtcggaa aagggaaaat actttcacca ccagctcaaa
     2461 ggattttatg cctttctcaa gaatgatcaa aatcaatcct acggggagat ccttgaaaaa
     2521 gatcccctgt attttgataa agtttatcct tatgccgtcg ccttaaaact cgaccagatt
     2581 tttataaaga aaatacgacc ctacagggag ttcgcccccg tatggtatgg ttattacggc
     2641 atgatggggg ccgggcgaaa atccaccatg gaagattttg gccgggattt ccagcctaaa
     2701 gaaatctctt cggcattcag caccgtgcaa ccggtttcca cgggaggcgg gggcggaggg
     2761 ggcttcggcg gcggcggcag ttccgggggc ggatttggag gagggggagg aagcagctgg
     2821 taaatgaaaa ttaagaatta agagaattaa ggaattaagg gaattaagga taaaaaaaaa
     2881 gagggtggag tgaactaccc tctttttgct ttttctctat ctgatccctg atcccttaca
     2941 actacatact tggagccttc tccccgcgat ggcaggtcgc acaggagatc atgtttttag
     3001 attgaaactt tttggtcata tactttttat tgatcccatt ggtcattttg accatgtccc
     3061 gggccatgtt tttttcaggc ttggtatcgg cgacaaaatc ccattggctg aatggagcgc
     3121 cttccttgct cacatggcaa tggttgcact tgaccccaag ggctttgttc catgactgca
     3181 tgatggcttt gaggtcatcc ccggcggtgc ctttgggcaa tactttcagg ttctttggtt
     3241 ctgtcggcca ggtccaggta tcttttggct gaaaactgaa ttcgcagaat acgggaacgg
     3301 caaagatcag ggccagcacc ggaagtttgt gagtgagttg cttcatgtta tatgattttg
     3361 agcgtaaagt acaaaatatt gcaaaagcac aaaaccggat aacctaaatc ttgaaggctc
     3421 gctgaaaata tcaattgcac agggcgggat cgccggaaat gttcagcggg tatccgggga
     3481 ccaatggtaa aaagacggga tcaaatcccg ggagccctca gggggaaagc cgctctacgc
     3541 tccatttttt gcggctgagg cgataagcga tccggtcatg cagacggtct tccctgccct
     3601 gccagaattc aaaataatcc ggcaccaggc tataaccacc ccagaagggg ggcaagggga
     3661 ccggttcata ttctttaaaa aacttttcaa gctcctggtt gatccgatca atgtattccc
     3721 ggttggggac ccgctggctt tggtccgaag tccaggcgcc tatctgggaa ccccttggcc
     3781 tggagtggaa atattcttcg gaaaggcttc ggcttacttt ttttaccttt ccttcgacgc
     3841 ggatctgtct ttcctgttcc ggccagtaaa acaaaaggga ggcctgtggg ttggatttta
     3901 aatccctgcc cttgcggctt tcatagtttg taaaaaacaa aaaccccatg gaggcatcgt
     3961 agtatttgag caggacgatc cggctcgagg gacggccggt ctctccgacg gtggaaagga
     4021 ccatggcatt gggctcggca ggatgggatt tcaggctttc ctcaaaccag gatttaaact
     4081 ggtcaaaagg gttgactgcg gcggtgagtt cactcaggct gcttttggaa taggatttcc
     4141 gcagatgggc cagggggctg tgtttcataa cattgattat gcttgtccgc tttcttccgc
     4201 agcttccgcc agggccttgg cttcgtaatc ctccaggccc agcctggctt ttaaccggga
     4261 aacgatcacg agcagggccg gtaccaggac cagggtgagc agggtcgcaa aacccagacc
     4321 gaagatcatg gtccagctca ggggacccca gaatgccacg ttgtcgcctc caaggtagat
     4381 attggggctc cattctgaaa acagggtcac aaaatcaata ttaaaaccga tggccagggg
     4441 gatcaaaccc aagatggtgg ccatggcggt caggattacc ggggtcatcc gggttctgcc
     4501 cgcttccacc acggcctccc ggatggaata gccttgcttc atcatcattt ccgcaaattc
     4561 gaccagcagg atcccgttcc gcaccaccac cccggccaaa gccacgatgc ctatgccggt
     4621 catgatgatt gaaaattcca tcctgatcat cgtgaagccc aggagcaccc cgatgatgct
     4681 gaatgggatt tccaccagga tgatgatggg cttgctgaag gaattgaact gggtcaccag
     4741 aatgagaaag ataaggccca gcgagatcaa aagggaccgg cctaaaaacc ccatggcttc
     4801 tgcctggtct tcctgttccc ccgcgaaccc aatctcaata ccgggaggcg cctggaatct
     4861 tgccgtcgcg gactggactt tggccaggac ctcattggga ttgtatccgg ccagcacgtt
     4921 ggaagagatg gttaccaggc gtttcagatt tttcctttta atggagccga cagtgttgct
     4981 gtattccagt tgggtcaggg cggccatggg cacctggcgc atttgtcctc ccatattcat
     5041 gtcgcggtag gtgatgctct ggttgagcag gaggttcaca ttgttccgct ggtcttcctt
     5101 aaaacggagc tggatgggat gttggtcctc cccgtcccgg agttttgaga cttcttttcc
     5161 aaagactgcg ctccggaggt cggaaccgat ctgggcggtg gaaatgcctt ctgtgtttgc
     5221 acgctgacgg tccacggtga ttttgagttg cggtttgctg atggaaaggt cggattttaa
     5281 ttcttccacc ccatcgatct ggagggagtc caggtaccgc ttcaggtttt gcgcggtttg
     5341 attcagttca tcgaattctt caccgacgat ttcaatactg atgggcttac ctgccggcgg
     5401 tccgctgttt tccttttcga cgatgatttc cgcgcccgga atgcctttca ccacttcccg
     5461 gatcttattg aggtattcca ctgtggattt gccatgcctt ttttcaaatt ccacaaaggc
     5521 cacggtcacc ttgcccaggt gggggctggt ttgctgttca aaattggacg gatcgcccgc
     5581 acctacggcc acattggata tcacggattc cacgatcggg ttgtcctccc cgaccactcc
     5641 aatgatccgg ttctccactt ctgccgtgac cgcattggta taggcctggt cggtgcccac
     5701 gggcagggtg atataggtgt atacaaaatt ggggtcggat tcgggaaaca gtcggacgcc
     5761 gggaatgtat ttgaaggcaa ggaatagtga aacgaacaac aagaagacgg taaaaagcaa
     5821 caggccaata ggccggtacc cttttaccgc cacccgcagc atagaggcgt aggcattctg
     5881 caccgcaggc catacggagg tctggaaccc gcggatcagt ccgttcaaga caaaacggtt
     5941 gagcaggtaa aagaggaaga tgcacagggc cagattacgg ataaaataat tttggccaag
     6001 gtgggaaagc aacaggacga ccccgaagaa caccagggtg acgatgaatt ttctgttgaa
     6061 tattttttcc tttttccctt cctcatgctt ttgcataaaa tccacggcaa acaccggatt
     6121 gatgatgtaa gccaccagca gggaggccag cagggtggtg atcagggtga tgggaaggcc
     6181 aaacataaaa ttgccgatga ggcccggcca gaatgccagg ggtatgaagg gggccaggga
     6241 ggtcagggta cccgaaagga ccgggaggaa tacttcgccg gcggccatct tggccgctct
     6301 ttttatcggc acttttccgt tgtcgaaaat ccggtgggtg ttttcaatga ccacgatggc
     6361 atcgtccacc acgataccca gggcaaggag gaatccgaat aggacaatca cgttgagggt
     6421 aaacccgtaa gtgggcatga ccataaaggc cacaaacatg gaaaggggca cggataaagc
     6481 cacgaaaacg gcgttggtag cacccataaa aaacatgagg atgaaggtga ccagcaggaa
     6541 tccgatcacg atggtattga tgagttcctt gagtcccgct ctcgtcttga ggctcatgtc
     6601 gccggtgatg ttcacattca ggtcggaggg gaatgcggaa gcctgcatcc gttcgctgat
     6661 cgccttgatt ttatccgatg tctcgatcag gttttccccg cttcttttga tgacgttgag
     6721 ggtgagcaca ttttttttat tgagccgggc ataactctcc tgctccatga aggtgtcctt
     6781 aatctcggcg atatccttca gatagaccac ggcgccggtg ccgccccgga cgatgatgtc
     6841 ccccagttga cgcgcgtctt tgtattcgcc gaccacactg atggcgcgtt tcacattgcc
     6901 catgtcgagc tggcctccgg aaatggtcat gttttccatt gcgatggccg tctcgatatc
     6961 ccgcatggtc acattggccg cggtcatttt gtacatatcc acattgacct ggatctcccg
     7021 caccagggcg ccgaccaggt cagcacgggt gatttccttg agggtttcga tctcgtcctt
     7081 gagttgctcc gcgtattttt taatccggtc caggtcgtaa tcgccggaga cgtggatgtt
     7141 catgatggga atgctggaca cgtcgatatc gatgacggaa ggatcctggg gcaggtcatt
     7201 cgggaggtcc gcccgggcgc ggtccacggc atcttttact ttcagtttgg cctccgccac
     7261 atccacatcg gtgccaaatt cggcgacgac cagggaaaag tcctgcaggg aactgcttcg
     7321 gatttttttg acccccgaaa tgcccttgag ctggttttca atgggtttgg caaccaggtt
     7381 ttccatatcg gtcggagagg tgccgggata caccgtattg accaggatct gggggaaggc
     7441 aacttccgga aactgttcct tgggcagccg gatatatgaa aaaatacctg cgatcgtgat
     7501 gatgatggtc aggatataga tactggtctt attgtcaata gcccagctgc ttggcttgaa
     7561 ttccttgata agatctttca tggtctggat tccggggatt tatgattaat aggtttggat
     7621 gctcagccgt tcgccttcga gcaggtcctg gtatcccagg gtaatcactt tgtggctggc
     7681 atcgatcccg gatttgatct cgaccaggtc attgtaaaac ttaccgatgg tgacggggcg
     7741 tttggaagaa tgccagtacc cgtccgtgga atccgctacc atgacatagt ccccctggct
     7801 gtctttttgc aggaggttga tgggcagtac ataggcattg gggttgctgt aatccacgat
     7861 tttaatgatg gcaatcatgt tgggcatcac cccgctcata ttcggaggca gtgcggcttc
     7921 ggcggaaaaa gtgcggttta cccggttgat gaccgggctg acataagtga tgcgcgaatc
     7981 atattctttt tggatgtcgg gaaaccagat tttgatcgcg ttcccgggtt tgatccgccc
     8041 tgcataggat tcgggaacat tgacggaaag tttcaatttt gaaaaattga ccagccgggc
     8101 aatgggcgcc ccgggcatga ccatctggcc cggcttgacc atcacttcgt caatcactcc
     8161 ggatatcgga gcttttaccc gggtcatgtc ccattgttcc tttgtggtgg caatcctctt
     8221 ctcgagcgat tctttgttgt ttttggcgct gagatactgg acttccgtgc cgatgttctg
     8281 gtcccagagg gctttttgtt tttgatacag gtcggtggca aattgcagtt gctgctcgag
     8341 ttcggcaagt ccttgctgga gcagggcgtc gtccagggtg agcacggttt gaccggcctg
     8401 gatatgatcc cccgttttga tcaggatatt ctgcgcgttg ccggccatcc tggaattgat
     8461 gtacaattcc tggtcagcaa gcaccgatcc ctggaggtcg atatagtttc tgaacagggt
     8521 ctgggggaat acccggatcc ctacattctt ggttttttca accttgcctt cggggtaaag
     8581 ttccagttcc agggccgctg cttctttttc aaggcttgcg atctggtttt tgagttccgt
     8641 gagccggctt tgtttgtccg gggtgggaga ggagcctgat ttggatgagc aggaggtacc
     8701 caatattgcc aggaaaagag atccggaaac gagggcggtt atttttttca tgtctgtgag
     8761 ttgataagta gttaatgggt ttattttccg agggccttat ccaggtcggt cttggtgaca
     8821 agcatgttat acagggcctg gatatagccg ctttgggcct ggaggaaaac ggtttcagcc
     8881 tggttgatct ccaggcttga gccgatgccc cctttatatt ttagttgggc ctggtcatat
     8941 attttctgcg ccaggtccag attgcgttga ctgccgtcca gggtcgttcg ggcgttgtca
     9001 aactggatcc tcgccgtact cacttccagg ttcatggccc tttcaaattc gttcatctgc
     9061 aaccggagtt tatcgagttc cacattggct ctctggtacc tggctttccg gttgaaaccg
     9121 tcgaagatgg gcagattaag cttaaagccg gcgatggaac tgggaaacca ttgggtctgg
     9181 tctttgtcaa tcaggttgtt tgactgggat ccataggaaa cgttggcaaa accggtaatc
     9241 accgggtagg cgctcagttt gatggtttgc atccggatct tgctgagttc ttcggccatt
     9301 tgcatggacc ggtattccgg ccgttgttca gggcgaaagg ccgataccag gtcggcattg
     9361 tctattttca accggtcggc caggacatcc aggttttccg taaggaggat ggtttctcca
     9421 aaaggaaaac ccatatgcat ccggagcacg ttgagggtga tctctgtcat ccggttggct
     9481 ttttcggctt ccagttgcat ggtctgcagg ctgagggaca gccggtcgac atccagttgt
     9541 tcgacaaaac cctgtttccg gacggcttcg gtctctttga caattttatc aagggtggcg
     9601 atgttttggt ccaggatgga tttgcttcgg atggcgatca gggctcccag ataggccttt
     9661 gcgacggact gcctgatggt gatatcggag acggaattct gccgggtgac cagttcctta
     9721 taaagtttgg tcgctttcag gccgacaaaa aaggagggat cgtagagaat ggtgttcatg
     9781 gccagggccc cggaaagaga attggaaagg ccaaagcggg cagggatgcc gccacccttg
     9841 ggatcttcga ccaggttgct gtccaacaaa ttgtaatgga ccagccggtt gtcgatggtt
     9901 gggcttaaaa aatccggcag gatggtggtg ggtatggcaa aataatggga gaagtcgatg
     9961 gtggcgttta ctttcgggat gccgatggat ttgtattcca tgagctgggc atcggcgtcc
    10021 accagattga gctgattttg tttgacctgg gcgctgtttt taagcgcgta ttgcaccgct
    10081 tcttcgagtg aaaaactgtt ttggcccatc aggggccggc ccgctgtgca aagcaggagg
    10141 aagaataggg aagtaagcag tttcgttttc ataaaatggg gttatccaat tttttaatat
    10201 aatgctcaca caattcccag cctttgggag tggtgatgcc gcccaggtga taccggatgg
    10261 tttccttaaa cactttttcc ggagaaaaca ggtgggcggg gaagatggtt tcgtcgatga
    10321 gggcggcgct gagggtgacg taatttttgg cgatgatgtc caccccgagt tccgaccggt
    10381 aatacccctg ggcaatgcca tccttcagat tttgccggat gactcccagg atgtgttcct
    10441 ggttgagttt gtccagtttt ttccaggatt cccagtagta tttccggatc tcgtatgcgg
    10501 tgttggggga tacctgacgc agcatcagga tcacatgctg ggcgatcagg atcatttctt
    10561 cgaccgcgtt ggcggcctgg gaccggatca gggccagcgc ttttttttct tcccggatgt
    10621 ggagatggac catcttttcg atcagggccg ctttgttggg cactttttca taaagcgttt
    10681 ttttagatat acccagtcct ttggcaaggt catccatggt ggtggacttg attccacagc
    10741 ggataaagag acttttggcc cggatgagaa tttcggagga cagatccatg ataagaaggc
    10801 gcaaaaataa ccggaaaaat cataggaaac ccgggtagtt taaaaagttt cctgaacagg
    10861 gacggacgat ggacgaatgg gtggaattgg aggacgaatg taggggggga agggacgaag
    10921 ggggaagggc gaacggtgat gggtgaaagg cgaaaggcga agggcgaagg gagcaaaagt
    10981 gtcggtgcct ccgaataaat caagtcccga ttccctggga cgaattactt ttgtgccaaa
    11041 tcacagaatc cgtttatgaa acttcaatcc aaaaccatcc tccttttatt ggcgggatcg
    11101 atctatgtca tggcctgcca gggtaaaaaa tcggatagcc aggatacggc agccgcccct
    11161 gctgaaccgg ccgcggccgc cccggcctcc aatgccgagg gtaaaaagat ttttgaaacc
    11221 tattgcgtgc tttgccatgg cgtagatggt aaactggggc tcaatgggtc caaggacctt
    11281 tcgatttctg tccttcccat cgaagaaaaa atcatccagg tgaccaatgg gaaaaatctc
    11341 atgacccctt ttaaagatat cctcacggaa gacgagatca aagcggtggt ggcttatacc
    11401 gaagagctta aagtaaaata atgccctcaa aggcctatcg tgtcctgggt ttattgtccg
    11461 gttcttccct ggatggcacg gatgtcgcca tttgcaaatt cgttatcgac cgggattggc
    11521 cgggcctggt ccgggaatgg catatcaccg aagcggccac cattccttat ccggatgaat
    11581 ggatcaccag gctaaaatca cttccctcgt ccacttcccg ggatctggta atcgcggata
    11641 ccgaactggg ccattattac ggcttaatct cccgggactt cattcagagg cacggcctgc
    11701 ccattgacct gatcgcctcc cacgggcaca ccatcttcca ttttcctgaa ttgaaaacca
    11761 ccacccaaat aggggacgga gcagccatca ccgcggagac gggaatcgat tcggtcaccc
    11821 aactccgggg aatggatgtg gctttcggtg cccagggcgc tccgctggcc cctcttggag
    11881 acaaatacct gtatccgggt tattcgtttt atctcaacct ggggggaatc gccaacctga
    11941 cgacctccgt ggaaggggca taccttgctt ttgatatttg cccctgcaac caaatcctga
    12001 atgccctggc aggggaggcg ggcttgactt atgatcacga tggagggctg gcggcctcgg
    12061 gcaaactcat ccctgattta ttacaacttt accaatccga cccttacctc accaggtctt
    12121 accccaaatc cctggacaat acctgggtgg tggaaaacca tgtgaaccct tctcttgggt
    12181 tcaatgcgcc gaccgcagat aagctgcata ccgcagtgga gtttatcgcc ctggagattg
    12241 ccaaagcctg tgccctcttc cccttgcccc cgaatgcagg gaggccctcg atgtttgtca
    12301 ccgggggcgg ggccctgaat cattacctgg tcacccgcat tcaggctctg gccggggaac
    12361 tcgaggtggt cctgccctcc cggaccatca ttgagtttaa agaggtttct ttcattgccc
    12421 tggccggttt gtttcggggt ctccggatac ccaatctttt tgcttccgtc accggagcgc
    12481 cgggggatac gattaatggg gcattgtata ccaatcaaac gacttccccc tggtcatgaa
    12541 taaaaaggtg ctcgtgaccg gagcgaccgg atttataggc aactataccg tccgtcatct
    12601 cctggcatcg ggctatgagg tatctgccct ggtccgcccg gaaagcctga ccctggattc
    12661 cgtggactcg tttcgtactc aggccaggat cttcgaagga gacatcctgg atcccgattc
    12721 cctggaggtc gccttccggg accaggacgc cgtagttcat gccgcagcac tcgtgagttt
    12781 caggccggag gataaatccc aactggcccg gatcaatatc gaaggcacag ccaacgtagt
    12841 caacacctgc ctggagcagc atattcccca cctggtccac atcagctcga tcgccgcctt
    12901 gggcgggcaa cccggccagg aatggtttga tgaaacttcc atttgggtga agaataagag
    12961 gcataccacc tatgcggaaa ccaaatacca ggctgaactg gaagtatggc gcggtaaagc
    13021 ggaaggatta aatgtctgca tcctgaatcc ctccctcgtg atcggcccct ggctgcccgg
    13081 tcatcattcc atgaagatct tttcccacaa tctgcctttt tatccttcgg gaggcaacgg
    13141 cctggtcgat gtccgggatg tggcggaagc cgtacggatc tgcctcgatc gcaaggtcaa
    13201 agatgagcgg attattctta acgggcataa tctcagttat aaggaaatgc tggaccggtt
    13261 ggcgacgcat aaaggtaaga aagggcctgc aatagccctg cccaggagcc tggccatggc
    13321 gggagttcag gttctcggcc tattcaaccg gattttcagg cgaaataccg tattccagcc
    13381 ccgcttgctt cactccgtat tttcaaaagc gggctttgac aatacaaaat ctgtcgaact
    13441 gctcggtttg aaatacaggc cggtggacga gagtattatt gcagttttgc agggatcaag
    13501 ggtcaacggt taaaggagaa aggagaaagg aaggcaggat ctcaaccctc aatcctcaac
    13561 cctcactcct catccctgcc caaccccggt atcacaatat tgtccgcttc cgtttttcgc
    13621 atctggatct ttttaaagga ttggtcggcc tggaaaaaga aaccggtgcc gacttccttg
    13681 ccgcggctcc actttacaaa tttattgctg tacacgatgg cacgaccttc atcccagatc
    13741 agttcctcac tttccagccg ggtactgtcg gccgatacca ggacgacctg gtcgcgggcg
    13801 gtcatgatcc ttttgttttc atcccggatg gcgtagcggg cagtcagcct ggctttcagg
    13861 cggagggaat catcataaaa ctcacaatag atgccttcgg tgaattcctg tttgggaata
    13921 ttattctgca gggttctttt gatcacgggg gctttgagca acacttccag ccgccccgaa
    13981 tccgtataat gcaggaccac atcccggacc atttcttcat tgcctttttt ctggtgggct
    14041 tcctggagga gattaaaatc attctcacag gcaccgagaa aaagaatggc cgctagacta
    14101 cctaagtaac gtaacatcgc ctttgattat ccgggtgatc cggtcgacaa acaatacgga
    14161 ggccgcatag gtatataccc cggggggtag gggatctccc ttgtaggtgc catcccattc
    14221 ccgtcgattc atcgttgatg tccaggtttc ttccctcata aaccaaacct ccccagcggt
    14281 taaacaccct gaggtactcg atggaagaaa tggcccggtt tccgtacaac ctgaagattc
    14341 tattattgct gtcctgatca ggtgtgaata cattgggggt atagagggaa cggtcgaggg
    14401 tcacgcggat gatcaatgaa tccgctccca tacaaccctt aatggtggtg accaccagtt
    14461 tatagccggt ggttccggga gggatgactt ccgtgttgag gcaggaatca caaacgatgc
    14521 cgtcgggcgg gacccaccgg atggaacgaa tggggtccgg agccatatag gtcccgttga
    14581 ggaccacttc ataacccagc cgtccattga ggtcaggacc cacatctacg gtcaccggag
    14641 gaggctcatc gatggtgacg gttgccgtat cctggcatcc ccgggcatct gtgatccgga
    14701 ccaggtagct tcctttattc aggcctttgt agatggattg acgcctgaat ttttcattgt
    14761 tgatactgaa ctggtaatcg ggggtgcccc ccatgccgga aatggtcacc gagccatccg
    14821 cgaccccatg gcagaccata tccatggcca gggcgttgat ttgcagggat tccaggtcct
    14881 cgcagcaggg ttggacattg atttcttttg aaaaggaata cgtacatcct ttatcggatt
    14941 ccaccaccag cacgatggtt tttaatccaa aggatttgta ccggacctcg tgggggcctt
    15001 tggtaaaatt gacttcggat ggcagggagc cgtccccgaa attccaggta tactttacga
    15061 tattgccggc cgagaaaaac gtgctgtccg aaatagtaaa ggattgttcg catttgagac
    15121 cggattcggg ctccacccgg ataagggtct ccgggccaag gaattcaccc aatcctccga
    15181 attcaataaa aaaaccatga ttggtggagg taaaattgtt gatcaggacc ccataactca
    15241 ttcctttcac catgtccagt tgtttcagga atccgtcttc accggggttg cagtcagggt
    15301 tttcttccgt gtcctgggcc gtattgttga ggccggtcgg gcccacgcag gcggtggcca
    15361 tacaccggac cattctttta ttggcgcagt tgttcaatcc gttaggaaat tcgaacaccg
    15421 caaaatcgat atcgtcattc ggtacatccg gggtgatctt gaaagtcagg ggcccgttgt
    15481 ttgcggcggt ccagcggtac caggttgaat tggattctga attgttgttg ttaggaatgg
    15541 agttattaaa atccaggcag gaattgtcgg cctcatcccg gtccaggccg gcgccgacca
    15601 cgctttccac gtgaaaggcg tctttactgc ataataccga accggtgacg caatccccgt
    15661 ttgtactcac cggcgggttg taattgtcca cgcagagctg aaaggtcccg gcattgttnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc attggtatag gcggtcacat
    15841 tcgaacagaa tttggtcaca tcggaaatcc ggaaagcatc caggcaaaaa tcattgtccg
    15901 gctgggcccg gaggaggaag gcagacaaaa tcccaacaca taaatagata tacttcaacc
    15961 tcattctcag ttaattccac aagttaaacg tcaataggac cgcaattgat ttcaccggtt
    16021 ttgtttcgga aatattaaaa ttgtgacctt taccctcata acagacgcaa tatatagaac
    16081 aatggttaga aaattcatcc caaacctgtt caccctcttc aacctcctga tgggatgcat
    16141 ttcactgtat tttgtcttta cccagcaatt ccggatcgta ttctggctga tcctgctggc
    16201 cgccctggcg gacgtcctcg acggaatggt ggccaggtgg ctgaaagtgg acagcggcct
    16261 gggagagcaa ctggattccc tggctgacgt ggtttctttc ggagtcgtgc ccggctccat
    16321 cctcttccag ctcatccggt atgcctggga gtccaatcac atgatcagta cgggcatgaa
    16381 cggtctatgg ctggcattcg gggggtttat ttttaccctg ggggccgctt tgcggctcgc
    16441 catttttaat attgacgaaa ggcagaccac ggaattcatc gggctggcta cccccggggc
    16501 atcggtggtc gtgctgggtc tcatgctggt ccatgtcaag ggggcgggtt ttatgtttga
    16561 cctgttgagc aatgaatacg tgctccttgg tttttccatc cttttgtttc tgcttatgct
    16621 ggcgcctctc cggatgttca gcctcaaatc catcacgaag gattggaagg ccaacggctt
    16681 gcccatcgct tttgtcctgc tggccatccc cactgccctg gttttcggtg gtccggccct
    16741 ggtcctactt cccctgcttt atgtactttt gtcggtatat ttattcagaa acacatgaag
    16801 tttagcgcac agatcagggt catgccgcac aaggttttgc tcgatcccca gggtaaagcc
    16861 gtcggcaaca acctcaaacg aattggcctg gaggcctgta ccaatgtgag ggttggcaaa
    16921 aatatacaga tggaggtgga agcgccggac caggacagcg ccgaaaaact ggtacggtcc
    16981 gcctgtgaaa aattgctgac caaccccatc atggaatatt tcgaatattc actgcaagct
    17041 caaaactgat gctgtacctg gtgccgacac ccatcggcga cctggaagac atcacctatc
    17101 gtgccctgcg ggtactccgg tccgtggatt atatcctttc tgaagacacc cgcacgaccc
    17161 aattcctgct tcagcattat cagatctcca aagaaacccg gtctttccat gattttaatg
    17221 agcatggccg gcttccggct gtattggccg atctcaaatc cggtaaaaac attgccctgg
    17281 tgagcgaggc ggggatgccg ggtatttccg atccgggctt cctgctggtc agggcctgca
    17341 ggcaggaaga ccttcctctt accgttttgc ccggaccctg tgccctgatc aacgccctgg
    17401 ttggttccgg tattccgacc gaaccctttc attttgaagg attcctgccg cataaaaaag
    17461 gcaggcagac ccgcctgaaa tacctgtccg gcctggactg cacttttatc ctctatgaat
    17521 ctccgcaccg gatcctccgg tgcctggagg aactgatcac gcattgcgga ccggaaagac
    17581 ccagcgcggt ctgcagggaa ctgaccaaaa aatttgaaga ggtccggacg gccaaccttg
    17641 gggatcaaaa aaaatatttt gaaaacgaag gggtggtgaa gggggaattt gtggtggttg
    17701 ttagcggccg gtaaactaat aataaaggct atcgtgcact ttaaagtaat ggacatagcc
    17761 ttaagccaat tcttagacgg ccaaaagggc taccccttaa aaaaaagtaa ctttgtcaaa
    17821 attcatttga gtatatgatc cggaggaccg tttatggctg cctggtgctg atcgtcgcag
    17881 ccaccatgct ggtttcgtgt aaaaaagaca accccaacta tcttgtagaa gtcagcgatc
    17941 cctcttttta ccaccggacc atgaaagaag tgacagacat catggtctat gatatttttt
    18001 ccccgcccgt agccagccgg atatacagca gggtggccat tgcgggttac gaggccatgg
    18061 tacccgggga cgcctcctat ttgagcctgg ccggccagtt gaatgcgatg gaggctatgc
    18121 ccaagccgga ggcgggaaaa gaatatgcct ttcccatttc cagcatcaaa gccatgatca
    18181 atgtggggcg gaccctgctt ttttcggagg acaaaatgga tgcctatgaa caggaaattc
    18241 tgtcagagta caggaaaata ggcgtccccg aagcggtatt ggcccggtcg cttgctttcg
    18301 gcgacagcat cagcgccgct gtcctgaaat ggacctccac cgacaattac aagcagtccc
    18361 ggacctttcc aaaattttca gtcagcaatg aggcggtccg ctggaagccg acccctccgg
    18421 cttatatgga cgcggtagaa ccacactgga ataaacagag gcctttgtgc ctggacagtt
    18481 gctcccagtt caagccgaag cctccggtgc ccttttcccg ggaccaggac agcgaatttt
    18541 ataaaattgc aaaggaagtc atggatgtgg tcaaccggca ggataccact tttaattcca
    18601 tcgcttcttt ctgggattgc aatccttttg tcatgcacca gcacggacat gtcatgtttg
    18661 ccaccaaaaa gatttctccc ggagggcact ggatcggggt ggccgggcaa atgtgtcgcc
    18721 agctcaacaa gtctttctcc gaaaccgccg aagtgtatgc gctcctgtcc attggcctat
    18781 acgacgggtt tatcatctgc tgggatgaaa aattccggag caacctggtg cgcccggaaa
    18841 cttatataaa tgaaaacatc gatccggagt ggaccccctt gctgcaaact ccgcctttcc
    18901 cggaacatac ctccggacac agcgtcgttt cgagcgtttc ggctgagatt ttaagtaatc
    18961 tcctggggga aaacatcgct tttacggaca gcgtggagat tgaatatgga ttgtttgccc
    19021 gttcttttcc cagtttcagg aaagcagccg aagaagcctg catcagccgg ttttacgggg
    19081 gcattcatta tatgccggcg attacggaag gggctacgca ggggatcaat ctgggtaaat
    19141 atattctgga gagattgaag acgagagtgg agtagttggt aagttattca gttttattca
    19201 gtta
//
LOCUS       DAOQXT010000094        18909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3300_length_18909_cov_6.600191, whole genome shotgun sequence.
ACCESSION   DAOQXT010000094 DAOQXT010000000
VERSION     DAOQXT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18909
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3300_length_18909_cov_6.600191"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKM27_14715"
     CDS             <1..311
                     /locus_tag="PKM27_14715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR08569.1"
                     /translation="LISNAIKFTDPNGRIQVRSSMNGKLVEIAISDNGVGIEKSKIEK
                     LFRIGENIKSVGTSGESGTGLGLILCKGFVEKHGGRIWAESEVGRGSEFKFTMPVYEE
                     "
     gene            complement(338..2650)
                     /gene="bglX"
                     /locus_tag="PKM27_14720"
     CDS             complement(338..2650)
                     /gene="bglX"
                     /locus_tag="PKM27_14720"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNR08570.1"
                     /translation="MKREKVSSALTSALTLALTLTLALTLLPTPLIQAQSGSMSTYIS
                     SLMAKMTLEEKIGQLNLLTQGGTIPTGSAVSTDVEAKIRKGQVGGIFGLTGPDKVRQA
                     QDLAMQSRLKIPLIFGLDVIHGYKTVLPVPLGLSCSWDTALIQRAARLSAREASADGI
                     CWTFSPMVDISRDPRWGRVAEGAGEDPFLGSEIAKAYVRGYQGSNMSGSEEIMACVKH
                     FALYGASEAGRDYNTVDMSRGRMFNDYLPPYKAAVEAGVGSVMTSFNDVEGIPASGNK
                     WLLTDVLRNLWGFRGLTVSDYTSINEMSNHGLGDLQRVSAMALNAGCNMDMVGEGYLT
                     TLEKSLKEKKVSLAQIDGACRKVLEAKYKLGLFKDPYKYIDESRPAGRILTPVNRTEA
                     RSIAARSMVLVKNENQILPLKPGGSIALVGPLADDKRNMLGTWAVSGNWEHSVTVLEG
                     IRNLAAGPGQVHYAKGCDITEDDFLAGQANVFGERVTRDGRSAQAMIDEAVAAARQSE
                     VIVAVLGEASEFTGEASSMSNIDLPASQKKLVEALKATGKPLVIVLMSGRPMTIEPEL
                     NASKGFLLSWFGGVESGNAVADVLYGKHNPSGKLSMTFPKNAGQIPIYYSIRSTGRPY
                     RGEPFRKFKSNYLDVSNEPLLPFGYGLGYSRFEYGAPVLDKTKMKAGEKLTASVTLKN
                     TGPYDGEETVQLYIRDQVASVTRPVKELKGFKKIFLRAGEEKKVEFEIRTEHLKFYNY
                     RLAHLAEPGEFSLGIGGDSGVKMEASFMLE"
     gene            complement(3035..4876)
                     /locus_tag="PKM27_14725"
     CDS             complement(3035..4876)
                     /locus_tag="PKM27_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNR08571.1"
                     /translation="MHYLSLEHAGKSFGEKVLFRDVSLMINKGEKIALIAPNGSGKSS
                     LLRMIAGIDKPEGENATYLLSKQVKLAFLDQDPGFNPESTVLDSVLDADIPAVKAVRQ
                     YELAQLHAAENPKALEVALHEMDVYKAWDVEARVKEILFKLNLQQLEQKVKTLSGGQV
                     KRLALAHVLLAQADLIILDEPTNHLDLDMIEWLEEYLSQESLTVLMVTHDRYFLDHVC
                     DTILELYNGSLHRYKGNYSAFLEKKDLMLENDQVRHDKLRQLYKQELNWVRRQPKART
                     TKAKSRVDQFAVIEDALRQSREQQKIRIDFGTARLGSKILELHYINKSFQGKPVIRDF
                     HYTFKRGERIGIVGPNGAGKSTLLNIIMNRIQPDHGKIITGSTVKFAYYEQSGLQLEE
                     DKKVIDVIRDIADNWKQPDGSVISVAQLLERFLFDRKKQQVYVSQISGGEKRRLYLLT
                     ILMQQPNFLILDEPTNDLDIFTLQVIEEYLAEYSGCLLIVSHDRFFLDKLVDHLFIFE
                     GEGHIRDYNGNYTQYRLEQEAKQYDDLRPKESGPLSASRQDHENQKAMRRIEKQLEQL
                     NQKKQEIQNRFDRPQVATEDLIKWSAELKEIESQLSSKEDEWLEMAG"
     gene            5141..8248
                     /locus_tag="PKM27_14730"
     CDS             5141..8248
                     /locus_tag="PKM27_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08572.1"
                     /translation="MKKSILFLSLVLLGLVEFSTPLNAQYFGRNKPHYKSFDFKLYET
                     PYFSIYHYLENKEALDRIALWTEQWYKMHQHVLRDSFFFKNPMIIYNDHADFQQTNAI
                     TGEIGVGTGGVTEAFRNRVIFPFTMTNQQTWHVLGHELVHAFQYDIVINGDSSSIRNL
                     ENLPLWMVEGLAEYMSIGRVDPFTSMWMRDAVLNNDVPGFKQLQNPNYFPYRYGQAFW
                     AFLAGLYGDDIIREMYSNTALYGLEIAAPVTLGLSMDALSERFQNSIRTYYEPFLGDK
                     KERLVGRELISSKNAGRLNVSPALSPNGKYVIFLSEKDLFSTDLYLADAATGKIIRKV
                     LSTVRDGHLDDLNYMESSGTWSPDSKQFAFIAYKKGIHAMVIKDVDNGRTVDEVHFKE
                     VPAMANPAWSPDGKSIVFAGLVQGQSDLYQYELRTKKLKRLTNDVYSDIQPNWSLDGK
                     SLVYATDKISMDRGRKHGKFNMNLAILDLATGQSRQLDFFYGANNLNPEHDDNGNIFF
                     LSDRDGYRNIYRYALASDSIFQMTDFLVGVSGITEWSPALSVGRKRDRIVYSYYAKNS
                     FTIYSATEERFLNKPVAPDDVDLEPGTLPMTNLGQKDIVSNNLSKADGYPQLPQDAAT
                     YMKKFAEKPVQNKFKLDYIGGSTGLGVGTGSFGTRTGLAGGVQMLFSDLFGNNQIYST
                     LALNGDIYDFGGQVTYINSKNKVTWGATLSHIPFQTGGVSYRLDTIQLGNQLWEVLRE
                     DLDILRIFEDQAGVFAQFPLSTTRRFETSISGNYRSFRLDRYPTYYDPLTGQFIAEDR
                     RERIPIESDIINIGGYLIKRTGFFNTGLAYVGDNSYFGMASPMAGYRTRLEVEKYFSG
                     FDFWAATVDYRKYWWKKPVSLAARFLYHARFGESANEFSPILIGNMGFVHGFYYTQLD
                     RIRERYGLTFEQLSGQKLLMGNVEVRLPFTGPERLAVIKSSYLLSELALFLDGGVAYN
                     DWNNIIFKEPSDPNYIGLSRFVMSTGVSLRVNLFGAFILEPYYAFPLLKNSRATFGLN
                     LVPGW"
     gene            8349..8996
                     /locus_tag="PKM27_14735"
     CDS             8349..8996
                     /locus_tag="PKM27_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNR08573.1"
                     /translation="MFPRIKICCISSEEEAKLAIRYGASALGLVGKMPSGPGVISDEM
                     IYRIARKVPPPIGTFLLTSETKPEDIIRHHRHTMTNTIQLVDTLEERSYDQLRAAMPY
                     VKLVQVIHVLDEGSVEEAIAASAEADALLLDSGNPNLDIKVLGGTGRTHDWKLSRKIR
                     ESVSIPVFLAGGLRPENVRRAADEVQPYGLDLCSGLKTEGKLDERKVEAFFREIY"
     gene            complement(9083..10711)
                     /gene="ade"
                     /locus_tag="PKM27_14740"
     CDS             complement(9083..10711)
                     /gene="ade"
                     /locus_tag="PKM27_14740"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNR08574.1"
                     /translation="MTWTVQGQMVDLEQGKIYGAEILISEGRIQNIQKTRSSKGPFLL
                     PGFVDAHIHIESSMLVPSEFARMALPFGTLATVSDPHEIANVLGVPGVYYMIENAEGL
                     PLEILFGAPSCVPASAFETAGARLDSQEVAALLSDPRVGYLSEVMNYPGVIHRDPELM
                     AMIRAAGKNHKPVDGHAPGLRGEALKSYVSAGIQTDHECFSYDEAREKLERGMKVIIR
                     EGSAAKNYTELAPLIGEFPERLMFCSDDKHPDDLQRGHINQLVARAVGDGFDLFDVLR
                     IACVNPRLHYPLTSGRLRVQDPADFILVDDLRDFRVRDVYVKGQRVVEDGECLLASKP
                     VPVINAFHAMPVSGEDFRLRALSEKIAVIEALDGQLITRALDAPALIREGYAVADPKN
                     DVLKIAVVNRYQAAKPATGFVRGFGLKTGAFASCVAHDSHNIVAVGTDDDAIARVVNK
                     IIEQKGGIAASLNSFVLGLELPVAGIMSPNDGVTVAKAYSMLDHFVRKDLKSPLRAPF
                     MTLSFMALPVIPALKMTDKGLFDGEKFEWTTVFQ"
     gene            10938..11447
                     /locus_tag="PKM27_14745"
     CDS             10938..11447
                     /locus_tag="PKM27_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNR08575.1"
                     /translation="MKKLFFVLFLAFGWTFASAQKIAIVDITQVLENLTEYKKAQEQL
                     DKLTATWKQEISDAREKIKVMYNKYQAEQVLLTDEQRRTKEEEIMAKEKEVMEMQKSK
                     FGPEGALFMKRQELVQPIQDKVYRAIQDYANTRGFDLILDKGSSAGVIFSSAALDKTP
                     DILKKLGIQ"
     gene            11496..12065
                     /locus_tag="PKM27_14750"
     CDS             11496..12065
                     /locus_tag="PKM27_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNR08576.1"
                     /translation="MKAIKYILAAAFIIAGMQGNAQKFGYLNSAGILSEVPEVKQAES
                     NLKAYQEQLSKQGQQKVEALQAKYQELARKEKQGEIAPKALQEQADKLKLEEEELAKL
                     EQEMQGQLAQKREALLQPILDRVNKAIQDVAKENGYTYIFDSSTGILLYADEASDVSA
                     LVRAKLGLPNLAAAKKDEAPAGANTSVKN"
     gene            12156..12605
                     /locus_tag="PKM27_14755"
     CDS             12156..12605
                     /locus_tag="PKM27_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNR08577.1"
                     /translation="MNLEEKINNDIRDAMKSKDQAWLRGARAIKAAILLAKTDGSGLA
                     LDQDREIKLLQKLVKQRQDSLAIYEAQNRADLAQVEREEIEIIQRYLPQPVTGEELES
                     FIRGLIQAEGASGIKDMGRIMGLATKGLSGKADGKTISEIVKKLLTA"
     gene            12785..13240
                     /locus_tag="PKM27_14760"
     CDS             12785..13240
                     /locus_tag="PKM27_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNR08578.1"
                     /translation="MNIYVANISYNATDAQLEALFAQYGAVKSAKIIMDRMSGRSRGF
                     GFVEMENDDEAKAAIAALNGQSWMEKELNVNEARPKEDRGPRPGGGSGGFRPRREGGY
                     GGGYRSGGDRGGFSRGGDRGGRTGGGDRGGYRGGNRDSSGDRDFNRDSD"
     gene            13563..14711
                     /locus_tag="PKM27_14765"
     CDS             13563..14711
                     /locus_tag="PKM27_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HNR08579.1"
                     /translation="MTPLERFFLMCSGSNPAILKKSPTDINKYIGIGATIFFTGVLAF
                     IASAYAIYTVFDSWIASLAFGLTWGLMIFNLDRYIVSSMKRQGSGWQDFFTALPRLLL
                     AVIISIVISKPLELKIFEKEINAELIIMEQEVYKTQEQKVRERFDSGITGLQQEVGTL
                     KTEVETLRAKRDELAEAARQEADGTGGSRQRNLGPIYKTKKQDADRAEAELNEAIARN
                     QPLIAEKENAIKSLQEASSSEVTGLQRAGLGGFASRIDALSRLSEKSGAIWIASLFIM
                     LLFIAIETAPIFVKLISSRSPYDYVLHKHEHVFEMNHQQLTSILATATRNKIAEENEI
                     NTFRTQATVKAEKQLIEHAIREEVERLKQQPAGWKAWWSKGRFLGAEG"
     gene            14784..15185
                     /locus_tag="PKM27_14770"
     CDS             14784..15185
                     /locus_tag="PKM27_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08580.1"
                     /translation="MKYWMRLWLLPFGLFSCEKADHQDPEILEGRYLTNPLLDFRCIA
                     LPADQLPTLEIVKNSGNTYDFKLHQYFPEKKITELRQILLAPSAQGFKLRYKNQDAGT
                     WKNEEFLDNRKILTLSAQLEDEFVYFVGEKK"
     gene            complement(15233..16288)
                     /gene="rlmN"
                     /locus_tag="PKM27_14775"
     CDS             complement(15233..16288)
                     /gene="rlmN"
                     /locus_tag="PKM27_14775"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNR08581.1"
                     /translation="MTTQALSTDIRAMNPEQLEAWVLGLGEPRYRAKQIYQWLWKKQV
                     TDFDRMSDLSKPFREKLKNSAVIRPLVLDKAQFSMDGTIKDRFTLYDGHKIESVLIPV
                     VRDHRYTVCVSTQVGCSLTCSFCATGKMKRIRNLDAAEIVDQYILVNRQCLEHYGSPL
                     TNIVYMGMGEPLLNYREVMRSVDLLTNENALGLSPRRLTVSTAGIAKMIRQLGDDQVR
                     FNLALSLHAADDVKRNKIMPINEQNHLEALMEALEYFYAKTQNEITYEYIAFDQFNDS
                     LADAQMLTRLCKRNFPVKVNIIEYNPVEGVELKKSGKGRLDEFASYLYNHGVNVTLRR
                     SRGKDIDAACGQLANKE"
     gene            16431..17441
                     /locus_tag="PKM27_14780"
     CDS             16431..17441
                     /locus_tag="PKM27_14780"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNR08582.1"
                     /translation="MNRLMAGCLLVFLSSCAASRYSIQEEDGTDYSNARNWAALPSME
                     DKADLIPDGLQTVKDAPVDVFFIHPTSFNNKNDHQQWNASLAVNKINRHTDRKPIKYQ
                     ASIFNQAGKVYAPRYRQVHLKAYFTKDTTRAKRAFELAYRDVRDAFRYYLDHYNAGRP
                     IIIATHSQGTTHGLRLMKEFFDGSPLQNKLVAAYLPGIPVPRDYFSHIPACNAPDATG
                     CVNSWRTFEYGYEPRFLAGEKPMIVTNPISWHSDTNYVDKSFHKGAVLWKFKGGILPQ
                     IADAQVHKNILWVHKPKFPWSFVYKSKNYHIADYNFFYLDVRENAILRRDVYLKGVRG
                     EE"
     gene            17556..18854
                     /locus_tag="PKM27_14785"
     CDS             17556..18854
                     /locus_tag="PKM27_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNR08583.1"
                     /translation="MSSEKQKIGLWTGTSLVVGNMVASGIFMLPATLASYGSLSLLAW
                     LLSGVGAISLALVYAWLSRIMPLARGGPYAYVRDSLGHFAGFQVAWIYWISIWCTNAA
                     LAIACTSYLTTFFPVLGNSTLAAALTCLGMIWFLTWLNTTGIRNAGRMQLITTILKII
                     PLLMIGIGGLFYIQASHFSDFNRSETSVLSAILTTTTLTFFAFTGLECATIPSREMDQ
                     PEKNIPKATVYGTSIGTIIYVLITLTVMGILPPEILVQSNAPLADAANEVWGGWARYL
                     VGAGAVISTFGGLNGWILMQGQIPAAAAADQLLPHIFARENKSKTPVFSLVGSSILVS
                     ALIFMNYQKSLASAYEFAILLSTLAILIPYLFSVIAFLIRGRAPGVMNWKPIHLIVAA
                     LAFLFSVIAVIGSGSDIVYWGFLLLIAGLPVYAWMKASNQ"
BASE COUNT         4687 a         5079 c         4669 g         4474 t
ORIGIN      
        1 atctgatttc caacgcaatc aagtttaccg atccaaacgg gaggatacag gtccgctctt
       61 cgatgaacgg caagctggtg gaaatcgcca tttcagataa cggagtgggg attgaaaaat
      121 caaaaattga aaaattattc cggatcggtg aaaacatcaa atccgtcggg acctccggag
      181 aaagcgggac gggcttaggc ttgatcctct gcaaaggatt tgtggaaaag catggcggaa
      241 ggatctgggc ggaaagcgaa gtgggccggg gtagtgagtt caaatttact atgccggtgt
      301 atgaggagtg aggagtgggg agtgagggtc tggaatctca ttccagcatg aaggaagctt
      361 ccatcttcac tcccgaatcc cctccgatac ccaggctgaa ttccccgggc tcggccaaat
      421 gggccagacg gtaattataa aatttcaggt gctccgtacg gatttcaaac tccacctttt
      481 tttcttcgcc cgcacgcagg aagattttct taaatccttt taattccttc accggccgcg
      541 tcacgctggc cacctggtcc cggatataca attgaaccgt ttcctccccg tcgtaaggcc
      601 cggtgttttt aagggtcacc gaagcggtaa gtttttcacc cgctttcatt ttggttttgt
      661 ccaggacagg ggctccgtat tcaaaacggg aataacccag cccatatcca aagggcaaca
      721 ggggctcgtt ggacacatcc aggtaattgg atttaaactt gcggaaaggt tctccccggt
      781 atggccttcc cgtactgcga atgctgtaat agatgggaat ctggcccgcg tttttcggga
      841 aagtcatgct gagtttcccg gaaggattgt gtttgccata caatacgtct gctaccgcgt
      901 taccggattc caccccacca aaccaggaaa ggagaaaacc ctttgaggcg tttagttcgg
      961 gctcaatggt catgggcctt ccgctcatca gcacgatgac caggggtttg ccggtcgctt
     1021 tcaaggcctc caccaatttt ttctggcttg cgggcagatc aatattggac atgctcgacg
     1081 cttccccggt aaactcagaa gcttcgccga ggacggcgac gatcacttcg gattgcctgg
     1141 ccgccgcgac cgcttcatca atcatggcct gggcgctgcg cccgtcgcgg gtcacgcgtt
     1201 caccaaagac attggcctgg ccggcaagga agtcgtcttc cgtaatatca caacctttgg
     1261 catagtggac ctgtccaggt ccggcggcca ggttcctgat gccttccagt accgtcacgg
     1321 aatgctccca gttgccgctg accgcccagg tgcccagcat attcctttta tcgtcggcca
     1381 ggggcccgac cagggcaatg gatccaccgg gtttcagagg gaggatttga ttttcatttt
     1441 tgaccaggac catggagcgg gcagcgatgg accgggcctc ggtgcggttt acgggggtga
     1501 ggatgcggcc ggccggccgg ctttcatcga tatatttata gggatctttg aacaggccca
     1561 gtttgtattt ggcttccagc acttttctgc aggcgccatc gatctgtgcc aggcttactt
     1621 ttttttcctt cagtgatttt tctaaagtag tcagataacc ttcccccacc atatccatat
     1681 tgcaaccggc attcagggcc attgcggata cccgctgcag gtcgccgagg ccgtgattac
     1741 tcatttcatt gatggaggta taatcgctga cggtcaggcc cctgaatccc caaaggtttc
     1801 gaaggacatc cgtgagcaac catttattgc ccgaagcagg aatgccctcg acgtcgttaa
     1861 aggaggtcat cacgctgccc actccggctt ccaccgcggc cttataaggg ggcaaataat
     1921 cgttgaacat ccggccccgg ctcatatcca cggtattgta atcccgtccc gcttccgaag
     1981 cgccataaag tgcaaaatgt ttgacacagg ccatgatctc ctcactgccc gacatattgc
     2041 ttccctggta gccccgtaca taggcctttg cgatttcact tcctaaaaac ggatcttccc
     2101 cggcgccttc cgccaccctg ccccaacgcg gatcccggga aatatccacc atgggtgaaa
     2161 aagtccagca aatgccatcg gcgctggctt ccctggccga aagccgggca gcccgctgga
     2221 tcagcgcggt atcccaactg caggataagc ccagcggaac cggcaggacc gtcttgtacc
     2281 cgtggatcac gtccagccca aagatcaggg gtattttcag gcggctctgc atggccagat
     2341 cctgggcttg tctcacctta tccgggccgg tcaggccgaa aatgccaccc acttgtcctt
     2401 ttcggatctt ggcttccaca tcggtgctca ccgccgaacc ggtagggatg gtcccaccct
     2461 gggtcagcaa attgagctgg cctatttttt cctccagggt cattttggcc atgagggagg
     2521 agatataggt ggacatcgaa ccggattggg cctggatcag gggggttggt agtaaggtta
     2581 aggctaaggt taaggttaag gctaaggtta aggcggaggt taaggcggag gagacttttt
     2641 cccgtttcat aatcattaat ttctgtcgga tataaaccta cggaatattt aatttaaatg
     2701 gtccctgacc ggaaggttta atccaaatga tcaatagacc atatcagtcc atgtaccagg
     2761 tattatagcc cacgatccct caaggcctgc ccctggaaga aaccgattgt tttaaacccc
     2821 gctacctgca aaaaaatgag ctccatacgg gataccaccc gaagctggtg gctttgcagg
     2881 agatcaggaa tttaaaaatc cggcaaagtg gatgatgaaa agaattggtt ttacgcccga
     2941 tcgctgattg ctgaatgctg actgctgatt gctgactatt gactattgac tcctgactat
     3001 tgactcctga ctattgactc ctgactactg actcctaccc cgccatctcc agccactcat
     3061 cctccttgct ggacaattga gattctatct ctttcagttc cgccgaccac ttgatcaggt
     3121 cttcggtggc cacctgcggg cggtcaaacc ggttctggat ttcttgcttt ttttggttca
     3181 gctgttccag ctgtttttca attcggcgca tcgccttctg attttcatgg tcctgcctgg
     3241 aagcgctgag aggtcctgat tctttgggcc tgaggtcgtc gtattgtttg gcttcctgct
     3301 ccagccggta ttgggtataa ttcccgttgt aatcacggat atgcccctcc ccctcaaaaa
     3361 tgaacaaatg gtccaccagc ttgtcgagga aaaaacggtc gtgcgatacg atgaggaggc
     3421 aaccgctgta ttccgcgagg tattcctcaa tcacctggag ggtaaaaata tcgaggtcat
     3481 tggtcggctc gtcgaggatg aggaaattgg gttgttgcat cagtatggtg agcaggtaaa
     3541 gcctccgttt ttcccccccg ctgatctggc tgacatagac ctgctgcttc ttgcggtcaa
     3601 aaagaaaacg ttccaggagt tgagctacgc tgatcaccga tccgtccggt tgtttccagt
     3661 tgtccgcgat atcccggatc acatcgatga ctttcttgtc ctcctccagt tgcaggccgg
     3721 attgttcgta ataagcaaat ttgacggtgg agccggtgat gatcttgcca tggtccggct
     3781 ggatcctgtt catgatgata ttgagcaggg tggattttcc cgccccattg gggccgacga
     3841 taccgatacg ctcgcccctt ttaaacgtgt aatgaaagtc cctgatcacg ggctttccct
     3901 gaaaagactt attgatataa tgcagttcga ggatcttgga acccaggcgg gccgtaccaa
     3961 aatcaatcct tattttctgt tgctcccggc tttgacggag ggcgtcttca atgacggcaa
     4021 actgatccac ccggcttttg gccttggtgg tgcgggcttt gggctggcgc ctgacccagt
     4081 tcagttcctg tttgtacaac tgccggagtt tgtcgtggcg gacctggtcg ttctccagca
     4141 tgaggtcctt tttttccagg aaagcggaat aatttccctt ataccggtga agcgatccgt
     4201 tgtaaagttc caggatggta tcgcatacat ggtccaggaa atagcggtcg tgagtcacca
     4261 tcaaaacggt aagcgattcc tggctgaggt attcctctag ccattcgatc atgtcgaggt
     4321 cgagatgatt ggtgggctcg tcgaggatga tgaggtccgc ctgggccagt aacacatgag
     4381 ccagggccag cctcttgacc tggcctcccg acagggtttt tactttttgc tcaagttgct
     4441 gaagattgag tttgaaaaga atctccttga cccgggcttc cacatcccag gctttgtaca
     4501 cgtccatttc gtgcagcgcc acttccaggg ctttggggtt ttcggccgca tgcaattggg
     4561 ccagttcgta ttggcgcacc gctttcacgg ccgggatgtc cgcatccagc acactgtcca
     4621 gcacggtgga ttccggatta aagccgggat cctggtccag aaaggccagt ttgacctgtt
     4681 tggaaagaag gtaggtagcg ttctcccctt ccggcttgtc gataccggcg atcatacgca
     4741 gcagggaact tttaccgctc ccgttcgggg cgatcagggc gattttctcc cccttgttga
     4801 tcatcaggga aacatcccgg aacaggactt tttcaccaaa ggactttccg gcatgttcga
     4861 ggctgaggta gtgcatcggg ctgcaaagat aagggaaggt caagagtcaa gagtcaagag
     4921 tcaagagtca agagtcaagg gtcaagggtc aagagtcaag agtcaagagt caagagtcaa
     4981 gagtcaaggg tcaagggagc ggctctccct gctcaaactt ctcctgagac ctctaacctc
     5041 tgacctctag cctctgacct ctaacctatt tcaaaaattt aagagatctt taaagatcaa
     5101 aagcgaccga ttttaagtta aagaaagaga agatcaattt atgaaaaagt ccattttatt
     5161 cctttccctg gtccttttgg ggctggttga gttcagtacc cctttgaatg cccagtattt
     5221 cggaaggaac aaaccccatt ataaaagttt cgattttaaa ttgtacgaga ccccttattt
     5281 cagcatctat cactacctgg aaaacaagga agccctggac cggattgcgc tttggacgga
     5341 acaatggtac aaaatgcacc aacatgtact ccgggactcc tttttcttca agaacccgat
     5401 gatcatatac aacgaccacg cggactttca gcaaaccaat gccatcaccg gagagatcgg
     5461 ggtgggcacc gggggggtca cggaagcttt ccgcaaccgg gtgatctttc catttaccat
     5521 gaccaaccag cagacctggc atgtgctggg gcatgagctg gtccatgcct ttcaatacga
     5581 tattgtcatc aacggggatt cttcctcgat cagaaacctg gaaaatctgc cactgtggat
     5641 ggtggaaggg ctggccgaat acatgtccat cggcagagtc gatcccttta cctccatgtg
     5701 gatgcgcgac gccgtactga acaatgacgt tcccggtttc aaacaactgc aaaacccaaa
     5761 ttattttcct taccggtacg gacaggcgtt ctgggccttc ctggccggcc tgtacgggga
     5821 tgacatcata agggaaatgt actccaatac ggcattgtac ggcctggaaa ttgcggcccc
     5881 ggtgaccctg ggcctgagca tggacgcctt gtcagaaagg tttcaaaact cgatacgcac
     5941 ttattatgaa cctttcctcg gcgataaaaa agaaagactg gtcggcaggg aactgatctc
     6001 ttccaaaaat gcaggcaggc tcaacgtctc ccccgccctc agcccaaatg gcaaatacgt
     6061 catcttcctt tcggaaaaag acttgttttc gaccgatctt tacctcgcgg atgccgctac
     6121 gggaaaaatc atccggaaag tgctcagcac ggtccgggat ggccacctgg acgacctcaa
     6181 ttatatggaa tccagcggga cctggagccc cgacagcaaa caatttgcct tcattgctta
     6241 taaaaaaggc atccatgcca tggtgatcaa ggatgtggac aacggcagga ccgtcgatga
     6301 agtccatttc aaggaagtgc ctgccatggc caacccagcc tggagccccg atggtaaaag
     6361 catcgtcttc gccggcctcg ttcagggcca gtcggacctc taccaatatg aactccgcac
     6421 taaaaaatta aaacggctga cgaacgatgt gtacagcgac atccagccca actggtcgct
     6481 cgacggaaaa tccctggtct acgccaccga taaaatcagt atggatcgcg ggcgaaaaca
     6541 cggcaaattc aatatgaact tggccatcct cgacctggcc accggccaaa gccgtcagct
     6601 ggatttcttt tacggggcca acaatctcaa cccggaacat gacgacaacg ggaatatctt
     6661 tttcctctcg gaccgggacg gttaccgcaa catataccgg tatgccctgg cttcggattc
     6721 catcttccaa atgacggatt tcctggtcgg ggtgagcggg atcaccgaat ggtcccctgc
     6781 tttgagcgta ggtcgcaaaa gggaccggat cgtgtattcc tattacgcca aaaacagctt
     6841 taccatatat tccgccaccg aagaacggtt cttgaataaa ccggtggccc ccgatgatgt
     6901 ggaccttgag cccggaactc ttcccatgac caacctgggc cagaaagaca tcgtgagcaa
     6961 caacctttcc aaagccgacg gatatcctca attgccccag gatgccgcaa cttatatgaa
     7021 aaaattcgcc gaaaaaccgg tacagaataa attcaaactc gattacatcg gcggaagcac
     7081 cgggttgggt gtggggacgg gaagtttcgg tacccgcacc gggctggccg gaggggtcca
     7141 gatgttgttt tcagacctgt ttggcaacaa ccagatttat tcgaccctgg cgctcaatgg
     7201 ggacatctac gacttcggag gacaggtgac ctatatcaat tccaaaaaca aagtaacctg
     7261 gggcgccacc ctctcccaca tccctttcca gaccgggggg gtcagttacc ggctggatac
     7321 cattcaactg ggcaaccagt tatgggaagt tttaagggaa gacctggata tcctgcggat
     7381 ctttgaggac caggccgggg tctttgccca attccccctt tccacgaccc ggaggtttga
     7441 aaccagtatt tcgggcaatt accgttcctt ccggctggac cgctatccca cctattacga
     7501 tccgttaacc ggccagttta ttgccgaaga ccgccgcgaa cggatcccga tcgaaagcga
     7561 tatcatcaat attggaggat acctgatcaa aaggaccgga ttttttaata ccggcctcgc
     7621 ctatgtaggg gacaattcat acttcggcat ggcttccccg atggcaggct accgcacccg
     7681 tctcgaggtg gaaaaatatt tttccggatt cgatttctgg gccgcaaccg tggattaccg
     7741 caaatactgg tggaaaaaac cggtgagcct tgccgcccgg ttcctctatc acgcaagatt
     7801 tggtgaaagc gccaatgaat tcagccctat cctgatcggc aacatgggct ttgtccacgg
     7861 cttctattat acccagctcg accggatccg cgaaagatac ggtttgactt ttgaacaact
     7921 cagcggccag aaactcctga tgggcaatgt cgaagtgcgc cttccgttca caggccctga
     7981 acgcttagcc gtgatcaaat ccagctacct gctttccgaa ctggccctct tcctggacgg
     8041 cggggtagcc tataatgact ggaacaatat tatcttcaaa gaacccagcg atcccaatta
     8101 tatcggcctg tcgcgctttg tcatgagtac gggcgtctcc ctccgggtca acctgttcgg
     8161 cgccttcatc ctggaaccct attatgcatt tcctttactc aaaaacagca gggccacctt
     8221 cggactaaac ctggtaccgg gatggtagat aggggtgagg agtgaggggt gagggtagct
     8281 caatcctcaa tcctcaatcc tcaatcctca atcctcaatc ctcaatcctc aatcctcaac
     8341 ccttaacaat gttcccccgc atcaaaatct gctgcatcag cagtgaagaa gaagccaaac
     8401 tggccatccg gtacggggcc tcggccctgg ggcttgtcgg gaagatgccc agcgggccgg
     8461 gagtgatttc cgacgaaatg atttaccgca ttgcccggaa agtcccgccc cccatcggca
     8521 ccttcttact gaccagtgaa accaaaccgg aagacatcat ccggcatcac cggcacacga
     8581 tgaccaatac cattcaactg gtggataccc tggaggaaag atcgtatgac cagctccggg
     8641 cagccatgcc ctatgtcaaa ctggtgcagg tgatccatgt cctggatgaa ggatctgtgg
     8701 aagaagccat cgccgcatcc gccgaagccg atgccctcct cctcgacagc ggaaacccaa
     8761 acctggacat taaagtgctt ggcggtaccg ggcgcaccca cgactggaaa ctgagccgta
     8821 aaatcagaga aagcgtgtcc atcccggtat tcctcgccgg aggactcagg ccggaaaatg
     8881 taaggagggc agcagatgaa gtgcaaccct atggccttga cctgtgcagc ggcttaaaga
     8941 cggagggtaa attggatgaa cgaaaagtcg aggcgttctt ccgggaaata tactgatgat
     9001 ccgataatct actaaccggc tacctgtaac tgactactga ctactgacta ttgactattg
     9061 actattgact attgactatt gactactgaa acaccgtcgt ccactcaaac ttctccccgt
     9121 caaacaatcc cttatccgtc attttcagtg cggggatcac cggaagggcc atgaaagaaa
     9181 gggtcataaa aggagcccgc agaggggatt tcaggtcttt gcgcacaaaa tgatcgagca
     9241 tgctgtatgc tttggctacc gtgactccat cgtttgggct catgatcccg gctacgggca
     9301 attccaggcc gagcacaaaa ctgttgaggg aagcggcgat ccctcctttc tgttcgatga
     9361 ttttattcac tacccgggcg atggcatcat catcggtccc tacggccacg atattgtggg
     9421 aatcgtgggc tacgcaggat gcaaaggctc cggttttgag cccaaagccc cggacaaatc
     9481 cggtcgcagg tttggcagcc tggtaccggt tgaccacggc aatttttaaa acgtcatttt
     9541 taggatcagc cacggcatag ccttcccgga tgagggccgg cgcgtccagc gcccgggtga
     9601 tcaactggcc gtcgagcgct tcgatcaccg cgatcttttc cgaaagcgcc cgaagccgga
     9661 agtcttcacc ggataccggc atcgcatgga atgcattgat cacaggtacg ggcttggacg
     9721 ccaacaggca ttccccatcc tcaaccaccc tttgcccttt tacatagaca tccctgaccc
     9781 tgaagtcccg gaggtcatcg accaggataa aatcggccgg atcctggacc ctcagccgcc
     9841 ctgaggtcag aggatagtgc agtctcgggt tgacgcaggc aatacgcaat acatcaaaca
     9901 gatcaaagcc atcgccgacc gccctggcca ccagctggtt gatatggccc cgttggaggt
     9961 cgtcgggatg tttgtcatcg gaacaaaaca tcaagcgctc cggaaattcc ccgatgagag
    10021 gagccagctc cgtataattt ttggccgcac tgccttcgcg gatgatgacc ttcatacccc
    10081 gttccagctt ttccctggct tcatcatagg aaaaacattc gtgatcggtt tgaatccctg
    10141 cggaaacata agatttgagc gcttccccgc gaaggcccgg ggcatggcca tccaccggct
    10201 tatgattttt accggccgcc cggatcatgg ccatcagttc agggtcccgg tgaatcacgc
    10261 cggggtaatt catgacttct gacaagtatc ccacgcgggg atcggacaac aaagcagcca
    10321 cttcctggct gtcaaggcgg gcgccggccg tttcaaaagc gctggccggc acacaggaag
    10381 gggccccgaa caggatctca aggggaaggc cttccgcgtt ttcaatcatg taatacaccc
    10441 ccggcacacc aagcacattg gcgatttcat gagggtcgga aacggtcgcc agggttccga
    10501 aagggagggc catgcgcgca aattccgaag ggaccagcat cgaactttca atatggatat
    10561 gggcatccac aaaacccggc aggaggaaag ggcctttcga ggaccttgtt ttctggatgt
    10621 tctggatacg gccttccgaa atcaggattt ccgccccgta aattttaccc tgttccaggt
    10681 ccaccatttg tccttgcact gtccaagtca tactcaccgg gttctttttc gttaaatcct
    10741 ttaatagtag gtccaaatta tgttattctt tttaatgcaa ataaccggtc ccgatttcta
    10801 aagatcttgt tttcagtttt ttacaaaatg gagggggttt gaccaccggt gacctggtgt
    10861 aactttttgt agatcaatac agttaccttg ttaaccacat gaataaaaat aataactttg
    10921 aatttaaatc accgatcatg aaaaaactat tctttgtcct ctttctggct tttggctgga
    10981 ctttcgcttc cgcgcagaag atcgccatcg tggatattac ccaggtcctt gaaaacctga
    11041 ccgaatataa aaaagcgcag gaacagctgg acaaactgac agccacctgg aaacaggaga
    11101 tctccgatgc ccgggaaaag atcaaagtca tgtacaacaa gtaccaggca gaacaagtcc
    11161 tcctcacgga cgaacaacgc cggaccaaag aagaggagat catggctaaa gaaaaagagg
    11221 tgatggagat gcaaaaatca aaattcggac ccgaaggagc cctctttatg aaaagacagg
    11281 aactggtgca acccatccag gataaagttt accgggctat tcaggactat gccaataccc
    11341 gtggatttga cctcatcctc gacaaaggga gttcggccgg cgttatattc tcaagcgcgg
    11401 ccctggacaa aacaccggat atactcaaaa agttgggaat tcaatagtac ctttgcggcc
    11461 caaaacagta tactctacta aacagcaaaa cagacatgaa agccattaaa tacatactcg
    11521 ctgcagcctt catcattgcg ggcatgcagg ggaacgccca gaaattcggc tatctcaaca
    11581 gtgccggcat cctttccgaa gtccctgaag tgaagcaggc ggaatccaat ctaaaggctt
    11641 accaggagca gctgtccaaa cagggtcagc agaaagtgga ggccctccag gccaagtacc
    11701 aggaactggc ccgcaaggaa aaacagggtg aaattgcccc gaaggccctt caggaacaag
    11761 ccgataaatt aaaattggag gaagaagaac tggccaaact cgagcaggaa atgcaaggcc
    11821 agctggctca aaaaagagaa gccctgcttc agcccattct cgaccgggtc aataaggcca
    11881 tccaggacgt agccaaagag aacggttata cctatatttt tgattccagt accgggatcc
    11941 ttctttatgc cgacgaggct tcggatgtca gcgcgctggt aagggccaaa cttggactcc
    12001 ccaacctggc tgccgccaag aaagatgaag cgcctgccgg ggctaatact tccgttaaaa
    12061 actagaccgg tccatctctc aaaaaaaacg aatattgcaa aacaaaagcc cagtttcctg
    12121 ggtttttgca tttcttacga agtgcccaat ttaaaatgaa cctggaagaa aaaatcaata
    12181 acgatatccg ggacgcgatg aagtccaaag atcaggcctg gctgcgtggt gcgcgggcca
    12241 tcaaagcggc catattgctg gccaaaaccg acggaagcgg gctggccctg gatcaggacc
    12301 gtgaaatcaa actgcttcag aaactggtaa aacaaaggca ggattccctg gccatctatg
    12361 aagcccagaa ccgggccgac ctcgcccagg ttgagcggga agaaattgaa atcattcaac
    12421 gctacctgcc ccaaccggtg acgggggaag aactggaatc ttttatcaga ggcctgatcc
    12481 aggctgaagg agcttccggc atcaaggaca tgggccgcat catgggtctc gccaccaaag
    12541 gactgagtgg aaaggcggac ggaaaaacca tttccgaaat tgtaaaaaaa cttctcaccg
    12601 cctgaacatt tcctgtaaat taccttactt ttgaacaaat taagtcgaac aaaaaggtta
    12661 accgcggata tttgcagcaa atgattttcc acttcaaatc caccctcctc taatatttct
    12721 taaaaccaac cgcacgtttt ttttcaaatc agtaatcaat tttcttattt aaattttttt
    12781 cagaatgaat atttatgttg caaacatcag ctacaatgct actgatgcac agctggaggc
    12841 cttatttgcc cagtacgggg ccgtaaagtc tgccaaaatt atcatggatc gcatgagcgg
    12901 acgtagccgc ggattcggct ttgtcgaaat ggaaaatgac gacgaagcta aagccgctat
    12961 tgcagccctc aatggccagt cctggatgga aaaagaatta aatgtcaacg aagcacgccc
    13021 taaagaagac aggggcccga gacccggcgg cggaagcggc gggttcaggc caagacgcga
    13081 aggaggctac ggagggggtt accgctccgg aggcgatcgc ggtggtttca gcaggggcgg
    13141 tgaccgcggc ggcagaaccg gcggcggaga ccgtggcggc tatcgcggag gaaaccgtga
    13201 cagcagcgga gaccgtgatt tcaacaggga ttccgattga tctaaacttt ttctagaata
    13261 ccaaaaggcc gcgggtatcc ctgcggcctt ttttattggg agcattcaat taaaatcagg
    13321 cagggacaaa ctttcagcta tcagttatca gttatcagct atcagctttc agcgctgccg
    13381 gttacttatg ctcccttacc cattaacctt tcacccttcg cccttcacct ttcgcctttt
    13441 ctccctttct cctaacaaac acttaaagtt gtagttcaat ttcccactcc ggtcgattaa
    13501 ataagggaag ccacaatttt caacgatacc ttgaggttta ttcataaaat caaaccaaaa
    13561 gcatgactcc actggaacgg tttttcctga tgtgttcagg atccaaccct gccattttga
    13621 aaaaaagccc gaccgatatc aacaaataca tcggcatcgg cgccaccatc tttttcaccg
    13681 gggtactggc cttcatcgcc agcgcctatg ctatctatac ggtttttgat tcatggatcg
    13741 cctccttggc ctttggcctt acctggggtt tgatgatctt caacctggac cgttacatcg
    13801 tttcgagtat gaaacgccag ggttccgggt ggcaggactt tttcaccgcc cttcccaggc
    13861 tgctgctggc cgtgatcatt tccattgtca tctccaaacc gcttgagtta aaaatatttg
    13921 aaaaggagat caatgccgaa ctgatcatta tggaacagga agtatacaaa acccaggagc
    13981 agaaagtccg ggaaagattc gattccggga ttaccggcct tcagcaggag gtcggcaccc
    14041 tcaaaaccga ggtcgaaacc ctcagggcca aaagggacga actggccgag gccgccaggc
    14101 aggaagcgga cgggaccggc ggatccaggc aacgcaacct cggccccatt tataaaacaa
    14161 aaaaacagga cgccgaccgg gcggaagcag aactgaatga ggccatcgcc cgcaaccaac
    14221 ccctgatcgc ggaaaaagaa aacgccatca aatcgcttca ggaagcctcc tcctccgagg
    14281 tcaccggact tcagcgggca ggcctgggcg gctttgcttc ccgcatcgat gcgctctccc
    14341 gcctttcgga aaaaagcggc gccatctgga tagccagcct ttttatcatg ttgttattca
    14401 ttgccattga aacggcgccg atctttgtca aactgatctc cagccgaagc ccctacgatt
    14461 atgtgcttca caagcacgaa catgtgtttg aaatgaacca ccaacagctt acctccatcc
    14521 tggccaccgc cacccgcaat aagatcgccg aagaaaacga gatcaatacg ttccgcaccc
    14581 aggccaccgt caaagccgaa aaacaattga tcgaacatgc catccgggaa gaagtggaaa
    14641 gactgaaaca acaacccgcc ggctggaaag cctggtggtc caagggccgg tttttgggag
    14701 ccgaaggtta aacccgcatt ttttctgcga tctgaccaac cttttccagg agggctccgg
    14761 tcttatttaa aaaagacgga gtaatgaaat actggatgcg tttatggtta ttgccttttg
    14821 gcttgttttc ctgcgaaaag gcggaccacc aagatccgga gatcctggaa ggcaggtacc
    14881 tcaccaatcc gctgcttgac tttcgttgca tcgccctgcc cgcggatcaa ctgcccactc
    14941 tggaaattgt gaaaaattca ggcaatacct atgatttcaa actgcatcag tatttcccag
    15001 agaaaaaaat aactgaattg cgccaaatcc ttctggcacc ttcggcacag ggtttcaagc
    15061 tccggtataa aaaccaggac gcaggcacct ggaaaaatga ggaattcctg gacaaccgta
    15121 aaatactgac cctgtcagca cagctggaag atgagtttgt atattttgtt ggtgagaaaa
    15181 aatagttgga tggtgggtag tggctggtcc acccactatt caccacccac ccctactcct
    15241 tattcgccag ttggccgcag gcggcgtcaa tatccttgcc ccggctgcga cggagggtga
    15301 cgttgacccc atgattatat aaataggagg caaactcgtc caaccggcct ttacccgatt
    15361 tttttaattc caccccttcc accggattgt attcgatgat attcaccttt actggaaaat
    15421 tccgtttgca gagacgggtc agcatttggg cgtcggccag ggaatcgttg aactgatcga
    15481 aggcgatgta ttcataggtg atttcgttct gggtctttgc ataaaaatat tccagggcct
    15541 ccatgagcgc ttccaaatgg ttctgttcat tgatcggcat gatcttattg cgcttgacat
    15601 cgtccgccgc gtgcagggac agggccaggt tgaaccggac ctggtcgtct cccagttgcc
    15661 ggatcatttt ggcaatcccg gccgtgctta ccgtaaggcg cctggggctg aggcccaggg
    15721 cattttcgtt ggtcagcagg tccacgctac gcatcacttc ccggtaattc agcaggggct
    15781 cgcccatgcc catgtagaca atattggtga gcggggatcc gtaatgttcc aggcattgtc
    15841 ggttgaccag gatgtactgg tccacaattt cggcggcatc cagattgcga atccttttca
    15901 ttttgcctgt ggcgcaaaaa ctgcaggtaa ggctgcatcc cacctgggtg gatacacaga
    15961 cggtatacct gtgatcccgg acgacgggga tcaatacgga ttcgatcttg tggccatcgt
    16021 aaagcgtaaa acgatccttg atggttccat ccatactgaa ttgggccttg tcgagcacga
    16081 ggggacgaat gaccgcactg tttttcaatt tttccctgaa cggctttgaa agatcgctca
    16141 tccggtcaaa atcggtcacc tgctttttcc aaagccactg atagatctgt tttgcccgat
    16201 acctgggttc ccccaagcca aggacccagg cttcgagttg ctcagggttc atggcccgga
    16261 tatcggtgct aagggcttgg gtcgtcatcg gcctgcaaag ataagggcaa ttcaacgggc
    16321 tccaatcctc aacctcaacc tcaacctcaa cctcaacctc aacctcaacc ttaacctcaa
    16381 ccttaacctc aacctcaaca tgccatccaa taccttttta cctttgcccc atgaatcgac
    16441 tgatggccgg ttgtttgctg gtgtttttaa gctcctgtgc cgcttccagg tacagtatcc
    16501 aagaagagga tgggaccgat tattccaatg caaggaactg ggccgctttg ccttccatgg
    16561 aagacaaggc cgacctgatc cccgatggcc tgcaaacggt gaaagatgcc ccggtggatg
    16621 ttttttttat acatcccact tcgttcaaca acaaaaatga ccaccaacag tggaatgctt
    16681 ccctggcggt caataaaatt aacagacata ccgaccggaa gcccataaaa taccaggcga
    16741 gcatatttaa ccaggccggc aaggtctatg ctcccagata ccgtcaggtg catctgaagg
    16801 cctattttac gaaggatacg acaagggcaa aacgcgcctt tgagctggcc taccgggacg
    16861 tgcgcgacgc tttccggtat tacctggatc attataatgc aggcaggccc atcatcatcg
    16921 ctacgcacag ccagggcacg acccacggcc tgcgtttgat gaaagaattc tttgacggga
    16981 gcccgctgca aaacaagctc gttgcggctt acctgcctgg cattcccgtg ccccgggatt
    17041 atttcagtca tatccctgcc tgcaatgcac cggatgctac cggttgtgtc aacagctggc
    17101 gcactttcga atacggatac gaaccccgtt tcctggctgg ggaaaagccc atgatcgtaa
    17161 ccaacccaat aagctggcat tccgacacca actacgtgga taaaagtttc cataaaggcg
    17221 ccgtcctctg gaaattcaag ggaggcatcc tcccgcagat cgccgatgcc caggtccata
    17281 aaaacatact ctgggtccac aaacccaaat ttccctggag ctttgtttat aaaagtaaaa
    17341 actaccacat cgcagactat aatttctttt acctggacgt gcgggaaaat gcaatactca
    17401 gaagggacgt ctatttgaaa ggagtgaggg gtgaggagtg aggattgatc caaacccctc
    17461 aatcctcatc cctcaaccct cacccctcaa tcctcacccc tcaaccctgt tttccttacc
    17521 ttgtccattt ttcagaccat tcgatccttc ctctcatgtc ttcagaaaag caaaaaattg
    17581 gcctttggac cggtacctcc cttgtcgttg gcaatatggt tgcctcgggc atttttatgt
    17641 tgcctgccac cctggcatcc tatggcagcc tgagcctcct ggcctggttg ctgtccggtg
    17701 tgggtgcaat aagcctggcc ctggtttatg cctggctgag tcggatcatg cctcttgcaa
    17761 ggggagggcc atatgcctat gtccgggaca gcctgggcca ttttgctggt ttccaggtag
    17821 cctggatcta ttggatcagc atctggtgta ccaatgctgc cctggcgatt gcctgcacaa
    17881 gttatctgac tacgtttttt cctgttttgg gcaatagcac cttggcagct gccctgacct
    17941 gcctgggaat gatctggttt ctgacctggc tgaacacaac cgggatccgc aatgcgggaa
    18001 gaatgcagtt gatcaccacc atcctgaaaa tcatccctct gctgatgatc gggatcggtg
    18061 gcttatttta tatccaggcc agtcattttt cggacttcaa tcgctcggaa acttctgtcc
    18121 tttccgccat tttgacgacc acaaccctta ccttttttgc ttttacaggt cttgaatgcg
    18181 ctacgatccc gtccagggaa atggatcaac cggaaaaaaa cataccaaaa gcgacggtat
    18241 acggaacctc aatcggcacc atcatttatg tactgatcac cctgaccgtc atgggaatcc
    18301 tgcctcctga aattctggta cagtccaatg cccccctcgc tgatgcagcc aatgaggtct
    18361 ggggcggctg ggcccggtac ctcgtaggag caggtgctgt aatatccaca ttcggaggcc
    18421 ttaacggctg gatcctcatg cagggtcaaa tacccgctgc ggcggcagca gatcaattgc
    18481 ttccccacat attcgccagg gaaaataaat ccaaaacacc ggttttcagc ctggtcggat
    18541 cgagcatcct ggtctcggcc ctcattttca tgaattacca aaagtccctg gcttcagctt
    18601 atgaattcgc aatcctgctg tctacattgg ccatattgat cccctacctc ttttcagtga
    18661 tcgcattcct catccggggc agggccccgg gtgtgatgaa ttggaaaccc atccatttga
    18721 tcgtggcagc cctggccttt ttattttccg tgatcgctgt aatcggttcc gggtcagaca
    18781 tcgtttactg gggattcctg ttgctgattg ccgggctgcc ggtatatgcc tggatgaagg
    18841 ccagtaatca atagtcaata gtcaatagtc aatagtcaat agtcaatagt caatagtcaa
    18901 tagtcaata
//
LOCUS       DAOQXT010000095        18550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3376_length_18550_cov_6.818924, whole genome shotgun sequence.
ACCESSION   DAOQXT010000095 DAOQXT010000000
VERSION     DAOQXT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18550
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3376_length_18550_cov_6.818924"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1337)
                     /locus_tag="PKM27_14790"
     CDS             complement(105..1337)
                     /locus_tag="PKM27_14790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR08584.1"
                     /translation="MAWKRNDQRLVFAGACLGMLIFGIVMITLGSTLPDLRERYRISE
                     LQAGSMFSLLPLGILTGSFLFGPVADRYGYKGLLMVHSFFILLGLVGIAYSSDYLLLM
                     VFVFLIGLGGGVLNGATNALVADISEQTKAARLGLLGVFYGIGALSMPTVLNLLKGGF
                     RADQIMAWVGWSILLVILFYAWIRFPKPKQAQGFSINQAVRLVKSRTLLLAGFFLFFA
                     SGSEGLANNWLASYLEDQHTLSREQSLFCLSLLVLSLTLGRLVLGRVLIKYSSQKVLF
                     ASLLMQFVAVGILYSGQGYAWSMGAACLLGLGFAAAFPVILGLVAELFAQWSGTAFSI
                     ALVIALTGNMLINYLMGFLSELRGIGMLHYLQWVAILSMMSLLFLFFRNHKENIYSQS
                     KNNQLSTCDFRKLLHFNN"
     gene            complement(1318..2235)
                     /locus_tag="PKM27_14795"
     CDS             complement(1318..2235)
                     /locus_tag="PKM27_14795"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR08585.1"
                     /translation="MSDFIAIDLGGTKLLGALVDEQGRVEHKIKRPVQGERGGEIADA
                     IHELIQALLEKSDQAGGIGISVPGIAYRNGVVWAPNLAGWDDFPLKDHLQTLGIPAIP
                     LEIESDRSCHLMGEVWKGAASGSEHAIFIAVGTGIGAGILADGHVLRGQNDISGAIGW
                     MALNPEYAAGYKERGCFETHASGYGLAFQARQLGRPEWNTEDIFPALEKGDPAAQKLM
                     NEAIRYWGMACANLVSIFNPGKIIFGGGVFGPAVRYIPIIYEEASRWAQPVSIRQVQF
                     LPSQLGDEAGILGAAWFAYKKWKDGLEKK"
     gene            complement(2265..3596)
                     /locus_tag="PKM27_14800"
     CDS             complement(2265..3596)
                     /locus_tag="PKM27_14800"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNR08586.1"
                     /translation="MKKMHLFLILLLGFALESFSQESKAARDQRMEWWRDAGFGLFIH
                     WGAYAIPAGIYNGTEVKGIGEWIMHTANIPIAEYEAFVQQFNPVKFDAKKWVAIAKKA
                     GVKYIVITSKHHDGFCLWDSQVSDYDIMDAAPFKRDILRELTDACKAAGIRICFYHSI
                     MDWHHPDAESKKDYTHQNSPNPDWNKYREEYLKPQVKELMTKYEPGVMWFDGEWIPEW
                     TEAQGKDLYNFIKELNPEIIINNRIGKGRKGMQGMSEYEDAAGDFGTPEQEILEGTSA
                     YDWESCMTMNDTWGYKKNDHNWKSAKQLIDNLIDVATKGGNYLLNVGPTAEGLIPPES
                     VKRLQEMGNWLKKNGEAIYNTRSRPNYKEGDHYKFTVSKDGKYTYVIADQFTGSTATL
                     KEINPRPGSDIYMLGLKKPLPWTQLSNGDYLITLPKKLPGKYAWTLKITNP"
     gene            complement(3707..4099)
                     /locus_tag="PKM27_14805"
     CDS             complement(3707..4099)
                     /locus_tag="PKM27_14805"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNR08587.1"
                     /translation="MPRILTIESLEPHYRCTCSNGVHRWYADEQESSGGGDTAPSPTE
                     ILLSAMGSCAINTLRMYAARKEWPLEKIRIRLSLETIKSESGTSYRIHEEVELTGPLD
                     QEQKDRLRSLLPKCPVTRVVTGQVELFY"
     gene            complement(4104..4739)
                     /locus_tag="PKM27_14810"
     CDS             complement(4104..4739)
                     /locus_tag="PKM27_14810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR08588.1"
                     /translation="MSIQSFTFNAFSENTYVLFNPDGSGLIVDPGMNEINERRRFQAF
                     LREKGITPTHILNTHCHIDHILGNKWCKETFQIPLWIPDGEQPMLDSLSQVAKMYGLP
                     AEPSPEPDYLMKEGDFLVSGTTRWQIISAPGHSPAGICLYDAEGKILIAGDVLFFESI
                     GRTDLPGGNHRLLLQNIQEKLMALPDEVVVYPGHGPATTIGHERVHNPFIN"
     gene            4844..5278
                     /locus_tag="PKM27_14815"
     CDS             4844..5278
                     /locus_tag="PKM27_14815"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR08589.1"
                     /translation="MKSLICLLLLLAGVAPLHAQALSEQENSVLRLSAEKFNYLNPEN
                     LDRLEPFLHDRLVFIHSNAMTETKEQMFRNLRDGKWKLRKVDVREASVRIFRKKTAVV
                     VGKGTFYASNAGHDIDIDLYYTETWVLDKKKWRLASRHASRL"
     gene            5303..7084
                     /locus_tag="PKM27_14820"
     CDS             5303..7084
                     /locus_tag="PKM27_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR08590.1"
                     /translation="MWRNPYLNLLATAWKYARNEKRRYLNIYGFYVITNLLNALRPIL
                     YGWFIHGLETQGVAVLRQAWLYVAAYLGLNLLFWLFHGPARVMERKLAFNLGRNFKME
                     LFHQALHLPVKWHQDHHSGTIINRIQKAYQALKDFFQDGFMFFEAFAKFILSFAAMLY
                     FSPFFGGLALVMGMLTIWIIFKFDKPLIQNMHERNEKEHLVSSNLFDTLSNINTVITL
                     RLEDQMKKSLSDRIAQVWPPYRKNVTLNEWKWFVADMLVALIYAIIILGYVSQQYQPG
                     ETFILGGLVTLVVYVHQFTSVFHDIAWQYNQIIRYNTDVLSVGKIQEAWRDLHPRESV
                     EDFPEEWKQIEISGLQFSHHGLYSDKNKTHSLHDIRLRLEKGKRIALIGESGSGKSTL
                     LALLRGLYPAEPGLLLNQDGHLYHDLAPVNRRVTLFPQEPEIFENTIRYNITLGLPVE
                     DAEINQVCETASFKEVLDQLPKGLESSIKEKGVNLSGGQKQRLALARGILAARTSDII
                     LMDEPSSSVDPRTELVIYRRLFHLFKDKVLVSALHRLHLLVHFDYIYVMQDGRILEEG
                     TFDHLRKNGRIFGELWKHQETSSAGGD"
     gene            complement(7125..7628)
                     /locus_tag="PKM27_14825"
     CDS             complement(7125..7628)
                     /locus_tag="PKM27_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08591.1"
                     /translation="MEAHTFDMPSSKLLKSILFTALVAGILDLAGAVVSYMISRGGGF
                     PKKILEYIAGGWYGSAALDGSLKMNIVGGISHFFIATCWTALFYFLYPRLRFLQKSRL
                     VSAILYGLVIWAVMNLAVLPLSAWNAPLRLMPFEAAKAAFILIVCVGLPVVVRTGSYY
                     KSIQTGA"
     gene            7681..9528
                     /locus_tag="PKM27_14830"
     CDS             7681..9528
                     /locus_tag="PKM27_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNR08592.1"
                     /translation="MRPSRFFWISLFSFVLASLLSGQDPSLRIEPPSWWIGMKNPAVQ
                     LLVHGDSISSLLPSVRYPGVSIRKISKTGNPNYLFLDLVIDKSTKPGSLTIFLGNKFK
                     AEWDLREREKGSSLRTGFGPGDAIYLITPDRFANGDPSNDASVMTLEKPNRKDQDGRH
                     GGDILGIVQNLEYIRDLGFTRIWCTPMLENNQPATSYHGYAITDFYKVDPRFGTNESY
                     RDLSRQARAMGMGLIFDVVLNHCGDHHWWMKDLPSRDWINNEGSYVNTNHRRETHQDP
                     HRSKSDEERMTAGWFVPTMPDLNQRNPFLTTYLIQNTIWWIEYAGLSGLRVDTYPYSD
                     KGFSSKWSKAILSEYPGFNMVGEEWSMNPMIIAYWQKGKKNHDGYVSHMPSMMDFPLN
                     QALVQALNEKESFNEGLIRLYHSLANDIVYADPSHLLIFPDNHDMSRFYTQIHEDFAR
                     WKLGLVFMATTRGIPQIYYGTEVLLTNPRSDAHGEIRADFPGGWTGDAKNAFTGMGLS
                     DKEKEAQVFLKSLLQLRKKTPAFQSGKLIHFAPEQGVYVYFRILGNEKYMVVLGKNRN
                     SIQIDLGRFVEILGASPSFTDVFTGQVLPLETARQLDAFGYRVMRVKSQ"
     gene            9665..9889
                     /locus_tag="PKM27_14835"
     CDS             9665..9889
                     /locus_tag="PKM27_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR08593.1"
                     /translation="MKEKKELTAIIEREGDYYVALCPELDIASQGDSIEESRTNLIEA
                     IELFFETASATEINQRLHDDVLITRVKVSV"
     gene            complement(10353..11054)
                     /locus_tag="PKM27_14840"
     CDS             complement(10353..11054)
                     /locus_tag="PKM27_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNR08594.1"
                     /translation="MKKLSFLLLALFTSQCAELQNLKLPIGQALSTEEIGGGLKEALT
                     NGISKGADLLSQVDGYFKSPYKVLLPEEALTVINRLKAVPGFNDLEANLTLRVNRAAE
                     LAAQKAKPIFVNAIKQMTFKDATNILMGADTSATSYLRTNTYDALYTEFRPVILNALN
                     EVHAADYWETAVTAYNKIPFVKKTNPKLDDYVTSKALTGLFSMIAKEEIGIRKDVSLR
                     TTDLLKKVFAKQDKK"
     gene            11211..11570
                     /locus_tag="PKM27_14845"
     CDS             11211..11570
                     /locus_tag="PKM27_14845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR08595.1"
                     /translation="MSTVKNFKELRIWQQSMSLVSKIYPVLQDLPVNERFGLAQQIRK
                     AAISVPSNIAEGQVRGTRKFIYFLNISLGSLAELETQLLLTVQLNFLSNEKTSPLIIE
                     LESLTRQIRALINKLKG"
     gene            complement(11648..12007)
                     /gene="folB"
                     /locus_tag="PKM27_14850"
     CDS             complement(11648..12007)
                     /gene="folB"
                     /locus_tag="PKM27_14850"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNR08596.1"
                     /translation="MNTHKGKIALEGLRLFAHHGYYEEERLLGREFILDIYVEADLAT
                     AGRTDQLGDTLNYEKIIDLCKEEMKIPSRLLEHVAHRIRERILALTGRDAVVKVRISK
                     PNPLLGVELGRFYVELG"
     gene            12709..13902
                     /locus_tag="PKM27_14855"
     CDS             12709..13902
                     /locus_tag="PKM27_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNR08597.1"
                     /translation="MKPSQREDRYQHHDYFQVDELLSEDHLMARQVVRDWVKKEVSPI
                     IEDYANRAACPTHLFKGLAEIGAFGPSLPVEYGGGGMDEIAYGVIMQELERGDSGVRS
                     MASVQGSLVMYPIYSYGSEEQKRKYLPKLGTGEFIGCFGLTEPDHGSDPAGMTTRIKD
                     AGDHYILNGAKMWITNSPLADLAVVWAKDEENTVRGMIVEKGNPGFSAPEIHGKWSLR
                     ASITGELVFEDVKVPKSQVLPGVKGMKGPLSCLSKARYGIAWGAIGAALDCYDTALRY
                     SKERKQFGRPIGQFQLTQKKLAEMITEITKAQLLTWRLGVLANQKKASPAQISMAKRN
                     NVHMALTVAREARQILGGMGITNEYPIMRHMMNLETVLTYEGTHDIHLLITGMDVTGL
                     NAFSG"
     assembly_gap    14155..14254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14905..16050
                     /locus_tag="PKM27_14860"
     CDS             14905..16050
                     /locus_tag="PKM27_14860"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR08598.1"
                     /translation="MNKIRLWSGIYSCGLLFAFFSAQAQPRSNTDSIRLVNPSFEDIP
                     KQSSPPLGWADCGFTGESAPDVHPSNFWEVSKPAIHGKTYVGLVVRNNDTWERISQML
                     ESPLQAGQCYHFSIFISTSENYKSRTNVGNNPLLRMQSDSIFNFNIPAVLRIWAGNIS
                     CGRSELLAESVPIENVEWKRYDFRLKPKANYNYIMFEAFFKTPVLFPYNGNILLDNAS
                     SLQPVPCNQPMEPPKPVAVAPKPKPPVWQAGKDTSKPPPPVVFRPKPKVGEIIKINNL
                     YFKADSDSIPPNSYQALDEIFHYLQDNESLVVEIGGHTNGLPKDDFCDSLSELRAKAV
                     AEYLMRKGIPRERMTYVGYGRRKPIDTNATREGRARNQRVELKILSS"
     gene            16050..17099
                     /locus_tag="PKM27_14865"
     CDS             16050..17099
                     /locus_tag="PKM27_14865"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNR08599.1"
                     /translation="MSLFKIGQERSREEIQEYLHATAWMPGETVHSTSKAGEGNMNVI
                     LRVSFFSGKSLILKQSRPYVNKFPSIPAPPERIETENAFYQLVRKHPELSDFMPRVIG
                     FDRPNRILALEDLGEGSDYTFIYKKDSSWPLDLNRDALRFLSILHHLPVPEGYPSNLA
                     LRQLNAEHLFDFPYRTDHGFDLDKVQYGLEDLARPYREDLLLREAIKGLKKIYLGQGG
                     RQLLHGDYFPGGWLQAGGGFKVIDPEFSFIGPAEYDLGIMMAHLFMARQDKNQVAGFC
                     SAYDSPSGFSDTLCWQFCGMEIFRRLIGLAQLPVDLSLEEKASLLKQAYEWVTGSGQA
                     SGHFGESGVSKPVTS"
     gene            complement(17122..18063)
                     /locus_tag="PKM27_14870"
     CDS             complement(17122..18063)
                     /locus_tag="PKM27_14870"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HNR08600.1"
                     /translation="MNHFISFRDVEDKEALLEQALSFARDPWQSEDAGRHKTLGMLFF
                     NPSLRTRLSTQQAAYQLGMKVIGLNADQGWKMEFEEGVVMNADRAEHVKEAAAVMSQM
                     VDLIAIRSFPGLTDRDKDYREEVMTGFCKYSRVPILNLESSTLHPLQSFADIMTMEQH
                     RSQDRKNTKVVLTWAPHIRALPQAVANSFSQWTLGYGYDLVITHPEGYELDEAFTPGA
                     RIEYDQDKAMEGADFVYAKNWSAYRDYGKILSQDPSWMITSKKMDLTSQGKFMHCLPT
                     RRNLEIADEVLDGPDSLVLKQARNRIFAAQAVLFGLL"
BASE COUNT         4553 a         4866 c         4653 g         4378 t
ORIGIN      
        1 aaatcggtct caaatactga ataaaaacct atagaatgga atggattgaa gatgagattg
       61 ccggtgtcct tgaaaaaatg gttaccgacg taagaacgaa aggatcaatt gttgaagtga
      121 aggagttttc gaaaatcgca agttgaaagt tgattgtttt ttgattgcga atatatattt
      181 tctttatggt ttcggaagaa aaggaagagc agcgacatca tactgaggat ggccacccat
      241 tgcaggtaat gaagcatgcc gatgcccctg agttcgctca ggaatcccat taaatagttg
      301 atcagcatat tgccggtgag ggcaatgacc agggcgatgc tgaaagcggt ccccgaccat
      361 tgggcaaaaa gctccgccac caaacccagg atcacgggga aagccgccgc aaagccaagg
      421 ccaaggaggc aggcggcgcc cattgaccag gcatatcctt gcccggaata taaaatgccc
      481 acggccacaa actgcatcag cagggaggca aaaagcactt tttgggaaga atattttatc
      541 aagacccttc cgagaaccag gcgccccagg gttaaggaga ggaccaaaag ggacaggcag
      601 aacagggatt gttcacggct cagggtatgc tggtcttcga gataactggc cagccagttg
      661 ttggccagcc cttcgctccc ggatgcaaaa aacaaaaaaa agccggccag gagtaaagtc
      721 ctggacttga ccaggcggac ggcctggttg atggaaaaac cctgcgcctg tttgggcttg
      781 ggaaaccgga tccaggcata aaacaggata accaataaaa tactccaacc cacccaggcc
      841 atgatctgat cggccctgaa accgcccttc aacagattca gcaccgtagg catcgaaagg
      901 gccccgattc cataaaaaac gcccagcaat ccaagccggg ctgccttggt ctgctcactg
      961 atgtctgcca cgagggcatt ggtagcccca ttcaatacgc cgccgcccag cccaatcaaa
     1021 aagacaaaga ccatcagcaa caggtaatcc gaagaataag cgatgcccac caggcccagc
     1081 aggataaaaa aagaatggac catcaaaagg cctttgtatc cataacggtc ggccacaggc
     1141 ccaaataaaa aagaaccggt caggatgccc aacggcaaca aggagaacat ggaaccggcc
     1201 tgcaattccg aaatgcgata cctctccctg aggtccggga gggtagaacc cagggtgatc
     1261 attacgatac caaatatcag cataccgagg caagccccgg caaacaccaa ccgctggtca
     1321 tttcttttcc aggccatctt tccatttttt ataagcaaac caggctgcgc ccaaaatgcc
     1381 cgcttcatct cccaattggg aggggagaaa ctgcacctgc cggatgctca ccggctgggc
     1441 ccacctggac gcctcttcgt aaataatggg tatataccgg accgcggggc caaatacccc
     1501 tcctccgaag atgattttcc ccggattaaa tatgcttacg aggttggcgc aggccatgcc
     1561 ccaataccgg atcgcctcgt tcatgagttt ttgcgcggca ggatcgccct tttccagggc
     1621 cggaaaaata tcttccgtat tccattccgg ccgcccaagc tgccgggcct ggaaagcaag
     1681 tccatacccg gaggcatggg tctcaaaaca tcctctttct ttgtagcccg cggcatattc
     1741 agggttgagg gccatccatc caatcgcgcc ggatatgtca ttttgcccgc gaaggacatg
     1801 gccatcggcc aggatgcccg ccccgatccc cgtcccgacg gcgataaaaa tggcatgctc
     1861 acttccgctg gcagcaccct tccagacttc acccatcagg tggcaggacc ggtcgctttc
     1921 gatttccagg ggaatagccg gaatgcccag ggtctggagg tggtctttga gagggaaatc
     1981 gtcccagcct gccaggttgg gcgcccaaac caccccgttg cggtaggcaa tgcccggaac
     2041 ggaaattccg atgccgcccg cctggtcaga cttctccagg agggcctgga tcagttcatg
     2101 tatcgcatcc gcaatctctc cacccctttc cccctggaca ggccgtttga tcttatgctc
     2161 caccctgccc tgttcgtcga ccagggcgcc aagcagtttg gtgcctccaa gatcaatggc
     2221 aataaaatcg ctcaaggttt ggatgggaat tcaggacttc gaaatcaggg gttggttatt
     2281 ttgagggtcc aggcgtattt gccgggcaat tttttgggca aagtgatcag gtaatcgccg
     2341 ttggacaact gggtccaggg cagcggtttt ttcagcccca gcatataaat atccgatcct
     2401 ggcctgggat tgatttcttt gagggtagcg gttgaacccg taaactgatc ggcaatcaca
     2461 taggtgtact ttccgtcctt gctcacggta aatttataat gatctccctc cttataattt
     2521 ggacggctgc gcgtgttata tatggcttcc ccgttttttt tcagccaatt ccccatttcc
     2581 tgcagccgtt tcacgctttc cgggggaatc agcccttccg ctgtaggccc tacgttgagc
     2641 aggtaattgc cccctttggt cgcgacatca atgaggttat caatcagttg tttggccgat
     2701 ttccagttgt ggtcgttttt tttgtaaccc caggtatcgt tcatggtcat gcaagactcc
     2761 caatcataag cggaagtgcc ttccaggatt tcctgctccg gggtgccaaa gtcaccggcc
     2821 gcgtcttcgt attcactcat gccctgcatg ccttttcgcc ctttgccgat gcggttgttg
     2881 atgatgattt ccggattgag ctctttgata aaattataca ggtccttgcc ctgcgcttcg
     2941 gtccattccg ggatccattc cccgtcaaac cacatgaccc cgggttcgta tttggtcatc
     3001 agttctttca cctgcggttt caggtattct tcccggtatt tattccaatc cgggtttgga
     3061 ctgttttggt gagtataatc ttttttggat tccgcgtcgg ggtggtgcca atccatgatg
     3121 gaatggtaaa aacatatccg gatccccgca gctttacatg catcggtgag ttcgcggaga
     3181 atatcccttt tgaagggagc ggcgtccatg atgtcgtaat cggaaacctg ggaatcccat
     3241 aagcagaacc catcgtggtg tttggaggtg atcacgatgt attttactcc tgcttttttg
     3301 gcgatcgcca cccatttttt agcatcgaat ttcaccggat tgaattgttg aacaaaggct
     3361 tcatattccg caatggggat attggccgta tgcatgatcc attcccctat tccctttact
     3421 tccgtaccgt tataaattcc ggctgggatg gcataagccc cccaatgaat aaacaaaccg
     3481 aatccggcat ccctccacca ttccatgcgc tggtccctcg ccgcttttga ctcttgggaa
     3541 aaactctcca gggcaaaccc caacagcaaa atcagaaata aatgcatttt tttcatatgg
     3601 tcagtattat aatatgacga atataggaat ttgtcccttt ctcctttctc ccttctccct
     3661 ttctcccttt attttccctt cgccttttac ccttcgcctt tcgtccttaa taaaaaagtt
     3721 ccacctgtcc ggtcaccacc cgggtcaccg ggcatttggg aagcaggctg cgcagacggt
     3781 ctttttgttc ctggtcaagc gggccggtca attccacctc ttcgtgaatg cggtaggagg
     3841 tgccgctttc ggatttaatg gtctccaggc taaggcggat gcggattttt tcaaggggcc
     3901 attcctttcg ggcggcatac attctcaggg tgttgatggc gcaggatccc atcgcactca
     3961 gcaggatttc agtgggcgaa ggagcggtat ccccaccccc gctcgattcc tgttcatcgg
     4021 cgtaccaacg gtgcacgccg ttggagcagg tacagcggta atgcggttcc agggattcaa
     4081 tggtcaatat tcttggcatg gattcagttt ataaaaggat tatgtacccg ctcatgcccg
     4141 atggtggttg ccgggccgtg gcctggatag accaccacct catcaggaag ggccatcagt
     4201 ttttcctgga tgttttgcag gaggaggcgg tggttgcctc ccggcaaatc cgtacggcca
     4261 atggactcaa aaaacagcac atcgcccgct atgagtattt tgccctctgc gtcatagagg
     4321 cagatgccgg ccggggaatg cccgggagcg gatatgattt gccaacgggt ggtgccgctc
     4381 accaggaagt ccccttcctt catcaggtag tccggttccg gggagggctc tgcgggcaag
     4441 ccgtacattt tggccacctg gcttaagctg tcgagcatgg gttgttcgcc atccgggatc
     4501 cataaaggga tctgaaaagt ttccttgcac catttattac ccaggatgtg gtcaatatgg
     4561 cagtgggtgt tgaggatatg cgtaggggtg atgccctttt cacggagaaa agcctggaac
     4621 cttctccgct cattgatctc gttcatcccg ggatcgacga tcagcccgga accgtccgga
     4681 ttaaacagga cataggtgtt ttcactgaac gcgttaaagg taaaggactg gatactcatg
     4741 ctaaagcggt acaaaggaaa tacaattttg gttcagcagg caggatttat agagaaggcc
     4801 tcgcgacctg atccggaacc caaaaacgaa ttatcttgaa gccatgaaat ccctgatctg
     4861 cctcctcctt ttactcgccg gcgtagcccc gcttcacgca caggccctct cggaacagga
     4921 gaactccgtg cttcgattgt ccgctgaaaa attcaactat ctcaatccgg agaacctcga
     4981 caggctggaa cctttcctcc acgatcgcct ggttttcatt cattccaatg ccatgacgga
     5041 aaccaaggaa caaatgttcc ggaatctccg ggacggcaag tggaaactgc gtaaagtcga
     5101 cgtccgggaa gcctccgtgc gcatcttccg gaaaaaaacg gccgtcgtgg tcggcaaggg
     5161 gactttttat gcgagcaatg cgggccatga tatcgatatt gatctctatt atacggagac
     5221 ctgggtgctg gataagaaaa aatggcgcct ggccagccgt catgcgtccc gtctctaagc
     5281 cggctttatc ctcctaccta ctatgtggcg caacccttat ctcaacctgc tggcaaccgc
     5341 ctggaaatac gcccggaatg agaagcggcg ctatctgaat atctatggtt tttatgtcat
     5401 caccaatctg ctcaatgccc tccgccctat cctgtacggg tggtttatcc atggacttga
     5461 aacccaggga gtagccgtac tgaggcaggc ctggctttat gtggccgcct acctgggact
     5521 aaacctgctg ttctggcttt tccacgggcc tgcgcgggtg atggaaagaa agctggcctt
     5581 taacctggga cgaaatttca aaatggagtt gttccaccag gcccttcatc tgccggtcaa
     5641 atggcaccag gaccatcaca gcgggaccat catcaaccgg atacagaagg cttatcaggc
     5701 cttaaaagac tttttccagg atggttttat gtttttcgag gctttcgcca aattcatcct
     5761 ctcctttgcg gccatgttat atttttcgcc cttctttggg gggctggccc tggtcatggg
     5821 catgctcacc atttggatca tcttcaagtt tgacaaaccc ctcatccaga acatgcatga
     5881 aagaaatgaa aaagaacacc tggtatcctc caatctgttc gacacgcttt cgaacatcaa
     5941 taccgtcatc acgctgaggc tggaggacca gatgaaaaaa agcctgtccg accggatcgc
     6001 gcaggtttgg cccccatatc gtaaaaatgt aaccctcaat gaatggaagt ggtttgtggc
     6061 ggacatgctg gtcgccctga tttacgccat aatcatcctg ggttatgtaa gtcaacaata
     6121 ccagccgggc gagaccttta tcctcggtgg cctggtgacc ctggtggttt atgtgcacca
     6181 gtttaccagc gtatttcatg atattgcctg gcaatacaac cagatcatcc gctacaatac
     6241 cgatgtgctg tccgtcggca aaatccagga agcctggcgg gacctccatc cccgggagtc
     6301 cgtggaggat tttccggagg aatggaaaca gattgaaata tccgggctgc aattttccca
     6361 tcacggcctt tattcggata aaaacaaaac ccatagcctg catgatattc gccttcgact
     6421 ggagaaaggc aaacgcattg ccctgatcgg ggagagcggc agcggcaagt ccaccctcct
     6481 ggccctgttg cggggtctct atccggccga accgggattg ctcttgaatc aggatggcca
     6541 tctctaccac gacctggctc cggtcaaccg gcgggtaacc ctttttcccc aggaacctga
     6601 aatttttgaa aataccatcc gttataacat caccctcggg ctcccggtgg aagacgccga
     6661 aatcaatcag gtctgcgaaa ccgcttcgtt taaagaagtc ctggatcagt tacccaaagg
     6721 cctggagagc agcatcaagg agaaaggcgt caacctctcc ggaggacaga aacaaaggtt
     6781 ggccctggcc cgtggcatcc tggccgcgcg gaccagcgac atcatcctga tggatgaacc
     6841 cagcagcagc gtggatcccc ggacggaact cgtcatctac cgacgcttat tccatttgtt
     6901 caaagataaa gtgctggttt ccgccctgca ccggcttcat ttgctggttc atttcgatta
     6961 tatctatgtg atgcaggacg gcagaatact ggaagaaggc acctttgacc atcttcgtaa
     7021 aaacggcagg atattcgggg aattatggaa acatcaggag acctcatccg ctggagggga
     7081 ttagtccgtt tcatataaag aaaaaacaat caagggacgg caattcacgc cccggtctga
     7141 atcgatttat aataactgcc ggtcctcacg accaccggca ggcccacaca aacgatcagg
     7201 atgaaggcgg ccttggccgc ttcaaagggc atcagcctta aaggagcatt ccaggcggac
     7261 aaaggcagta cggccaggtt catgaccgcc cagatcacca ggccataaag tatcgcgctg
     7321 accaaacggc ttttttgcag gaaacgcaga cgaggataaa ggaaataaaa caaagccgtc
     7381 cagcaggtgg caatgaaaaa atgggatatg cccccgacga tattcatttt gaggctgccg
     7441 tcgagcgccg cgctgccgta ccaacccccg gcaatgtatt ccaggatctt tttgggaaaa
     7501 ccgcccccac ggctgatcat atacgaaacg acggctcccg cgaggtcaag gattccggcg
     7561 accagggccg tgaagaggat ggacttgagg agtttggagg atggcatgtc gaaggtatgc
     7621 gcttccaaaa tacggattta taccctgacg gagtagggca tggatttgta tatttgatca
     7681 atgcgcccaa gccgattttt ctggatatca cttttcagtt ttgttctggc ttccctctta
     7741 tccggccagg atccttccct gcgcattgag ccgccctcct ggtggattgg gatgaaaaat
     7801 ccggcagtcc agctattggt gcatggggac agcatctcca gccttctccc ctcggttcgt
     7861 tatcccggcg tttcgatccg caaaatttcc aaaaccggaa atcccaacta cctcttcctg
     7921 gatctggtca tcgataaatc aacaaaaccc ggcagcctga cgatcttttt aggaaataaa
     7981 tttaaagcgg aatgggacct ccgggagagg gagaagggct cctccctgcg caccggtttc
     8041 ggccccgggg acgccattta cctgatcacc cccgaccgtt ttgcaaatgg ggatccgtcc
     8101 aacgacgcct cagtgatgac cctggaaaaa cccaaccgaa aggatcagga tggaaggcat
     8161 ggaggggata tcctcggtat tgtccaaaac ctggaataca tccgggacct tggcttcacc
     8221 cggatatggt gcaccccgat gctcgaaaac aaccagcccg ccacttccta tcacgggtat
     8281 gcgatcaccg acttttataa ggtggatccg aggtttggga ccaacgaatc ttaccgcgac
     8341 ctgtcccggc aagcccgggc tatgggcatg ggcctgatct tcgacgtagt gttgaatcac
     8401 tgcggcgatc accattggtg gatgaaggac ctgccttccc gcgactggat caataatgaa
     8461 gggagctacg tcaataccaa tcaccggcgg gagacgcacc aggaccccca ccgctcaaag
     8521 tccgatgagg aaaggatgac ggcaggctgg tttgtgccca ccatgccgga tttgaatcaa
     8581 agaaatccct tcctgacgac ctatctcatt caaaacacca tctggtggat tgaatacgcc
     8641 ggtttgagcg gcttgcgggt ggacacttat ccctattcgg ataaagggtt ttcctcaaaa
     8701 tggtccaaag ccatcctgtc ggaatatccc ggattcaata tggtgggtga ggagtggtcc
     8761 atgaacccca tgatcatcgc ctattggcaa aaaggcaaga aaaaccacga cggctatgta
     8821 tcccatatgc cctcaatgat ggacttcccg ctcaaccagg ccctggtcca ggccttgaat
     8881 gaaaaggaaa gtttcaatga aggcttgatc cggctgtacc acagcctggc caatgatatt
     8941 gtttatgcgg acccgtcaca tctgctcatt ttccccgaca atcacgacat gagccgcttt
     9001 tatacccaga ttcatgaaga ctttgcccgc tggaagctgg gcctggtttt tatggcgacc
     9061 acccggggca tcccgcagat ctattatggt acggaggtcc tgctgaccaa tccccgttcc
     9121 gatgctcatg gggagatccg ggctgatttc cccggaggct ggactggcga tgcgaaaaat
     9181 gcatttacgg gaatgggact ttcggataag gaaaaagagg cccaggtatt tttaaaatcc
     9241 ctgcttcaac tcagaaaaaa gacccccgcc ttccaatcgg gaaaactgat ccactttgcc
     9301 ccggaacagg gggtctatgt ctatttcaga atattgggaa atgagaagta tatggtggta
     9361 ttggggaaaa acagaaattc cattcaaata gacctcgggc gttttgtgga aatcctggga
     9421 gcatctcctt cctttacgga tgtttttacg ggacaagttt tgcctttgga aacggccagg
     9481 cagttagatg catttgggta tagggtgatg agggtcaaaa gtcaatagtc aatagtcaat
     9541 agtcagcagt cagcaatcag tagtcagtag tcagtagtta gcaggactat ctcgttgctc
     9601 gtttctcgct tctcgcccct tgatcaaaag gaccggattt tctgtatatt tgaattgaat
     9661 atacatgaaa gaaaaaaaag aactcacagc aataatagaa cgggagggcg attattatgt
     9721 tgccttgtgc cctgaactcg atatagccag ccaaggcgac tccatcgaag agtcacgcac
     9781 caacctgatt gaagcaattg agctcttttt tgagaccgca tccgcgacag agatcaatca
     9841 aagacttcat gacgacgtac tcattaccag ggtcaaggta tccgtgtaaa tgaatacgtt
     9901 ttccggaaag gaggtttgta atattcttaa gaaacatgga ttcaaggaag tcaggcaacg
     9961 cggcagccac atcatcatgc aaaaaccctt agggaacagc accattaccg taccagtacc
    10021 taatcataaa gaactgagat taggaacact tcggtcaatc atcaggcaaa gcgggatcga
    10081 taaatcctct ttcgaatagg ctctaaaatc caatagtcag cggtcagtag tcagtagtta
    10141 gcaagttagc tggttcataa gttcgcaagt ctagctcgct gctcgcccct ttgatcagtg
    10201 cggcagtccc ctttcagggg tcaggggtgt gcggatgaag tagtaatcag gaatcagtgg
    10261 tcagtagcaa gttagcaggg cgtttctcgt ttctcgtttc tcgcttctcg cttctcgctt
    10321 ctcgctgctc gctgctcgct tctcacatcc aactacttct tatcctgctt agcgaacact
    10381 tttttcagca gatcggtggt cctcaggctg acgtctttgc ggatgccgat ttcttccttg
    10441 gcaatcatgg aaaagagtcc ggtgagcgcc ttgctggtca cgtagtcatc gagtttggga
    10501 ttggtctttt ttacaaaggg gatcttgttg taggcggtca cggcagtttc ccaatagtcc
    10561 gcggcatgga cttcattgag ggcgttgagg atcacgggcc tgaattcggt gtacagggca
    10621 tcataggtat tggtcctcag gtaactggtg gcggaagtgt cggcacccat caggatattg
    10681 gtcgcatcct tgaaagtcat ctgtttgatg gcattgacaa aaatgggttt ggctttctgg
    10741 gcggccagct ccgcggcgcg gttgaccctc agggtgaggt tggcttccag gtcgttgaag
    10801 ccgggcacgg ctttcagccg gttgattacg gtcagggcct cctccggaag caggacttta
    10861 tagggacttt taaaataccc gtccacctgg ctgagcaggt cggcgccctt gctgatcccg
    10921 ttggtgagcg cttccttgag cccgcccccg atctcttcgg tcgatagggc ctggccgatg
    10981 ggtaatttca ggttttgcag ttcggcgcac tgggaggtga aaagggcgag gaggaggaag
    11041 gaaagttttt tcattctttt tttaatttct aaaacgcaaa agtattggat tggggggtta
    11101 tggaagggtt aaaatggtgg gtggtatccc gctaccgcgg gtggtggatg gtgggtggtg
    11161 gatggtggat gactacattt tttctacctt aatatactaa aacacacacc atgagcactg
    11221 taaaaaattt taaggagctt cggatttggc aacaaagtat gtcattggtt tcaaaaattt
    11281 atcccgtcct gcaggatctg cccgtgaatg aaagattcgg attggcccag caaatccgta
    11341 aagcggcaat cagcgtccca agcaatatag ccgaaggaca agtaagggga accagaaaat
    11401 ttatttattt cctaaatatc tcattaggtt cattggctga actggaaaca caattgttgt
    11461 tgactgttca attgaatttt ttgagtaatg aaaagacatc tccattgata atagaattgg
    11521 agagtctgac ccgtcaaata cgagcattga taaataaatt aaaaggataa aagacatccg
    11581 accatgataa gaccactggg atttcagggc catccaccaa caaccatcca ccatccacca
    11641 accaccccta tcccagctcc acataaaacc tccccagctc cacccccagc aagggattcg
    11701 gcttggaaat cctgaccttc acaacggcat cccgcccggt cagggccagg atcctttccc
    11761 ggatacggtg tgcgacatgc tccaggagcc tggaagggat cttcatttcc tctttgcaga
    11821 ggtcgatgat tttttcgtag ttgagggtat ccccgagctg gtcggtgcgg cctgcggttg
    11881 caaggtcggc ctcgacatag atatccagga taaattccct gcccaggaga cgctcttctt
    11941 catagtaccc gtgatgggca aagaggcgca agccttcgag ggcgatcttc cctttatgcg
    12001 tattcattga gtaattaaat tgatgaatcc gaatacaaga tacgaacagt cggatagagc
    12061 gatggtcgca tcatgggata taaccaagga ttgaatgagc aattctctct gcgatttctc
    12121 tgcgccctct gcgtcctcgg cggttaaacc cctacaaaca ccaacggcga taaagtccct
    12181 gattaaaaat cgattccttt ctctgcgatt cctctgcgtc ctctgcggtt cattaaatag
    12241 aacttacttc ctttccaacc aaaagaatcg aaggaaggaa tctttcatct agccgccaag
    12301 atcgcgaagt acgctgaggc tctgcaaaga catttcatta ccccttttcc tctgcgacct
    12361 tcgcgccctc tgcggtttat taaatggaat attcttcctt tccagcgaaa agaatcgaag
    12421 ggagaaatcg ttcgtttaac cgccaagtgc gcaaagtacg ctgaggttcc gcaaagacat
    12481 ttcattaccc ttttccctct gcgcctcctc tgcgaccttc gtgccctctg cggttaaaca
    12541 tgtgcaacca ccaacggcga taaagcactg attaaaatcg atcctttctc tgcgaatcct
    12601 ctgcgccctt tgcgacctcg gcggttaacc atatggaaac atacgcgggg aattgtagtt
    12661 tataccccaa tggaggcccg atccctgcga ttcttctatt tttgccccat gaaaccatcc
    12721 cagcgcgaag accgctatca gcaccacgat tatttccagg tcgatgaatt gttgagtgaa
    12781 gaccacctga tggcccggca ggtggtcagg gactgggtca aaaaagaagt cagcccgatc
    12841 attgaagact atgccaaccg tgcggcctgc cctacccatt tgtttaaagg cctggcggaa
    12901 atcggcgcct ttgggcccag cctgcccgtg gaatacggcg gaggaggcat ggacgagatt
    12961 gcctatggtg tgatcatgca ggaactggaa cggggggaca gcggcgttcg ttctatggcc
    13021 tcggtgcaag ggtccctggt gatgtatccc atctattcct acggcagcga ggaacagaaa
    13081 agaaaatatt tgcccaaact cgggaccggg gaattcatcg gttgtttcgg gctcaccgaa
    13141 ccggaccacg gatccgaccc tgcgggcatg actacccgca ttaaagatgc cggcgatcat
    13201 tatatcctca acggggccaa aatgtggatc accaattccc cgctggccga ccttgccgtg
    13261 gtctgggcca aggacgaaga aaataccgtg cggggcatga tcgtagaaaa aggcaacccc
    13321 ggcttcagcg cccctgaaat ccacggcaag tggtcgctcc gtgcatccat caccggcgaa
    13381 ttggtcttcg aggatgtcaa ggtgcccaaa agccaggtcc tgcccggggt caaaggcatg
    13441 aaaggccccc tgtcctgcct ctccaaagcc cgctatggca ttgcctgggg agccatcggg
    13501 gctgccctgg actgttatga cacggcgctc cggtacagca aggaaaggaa acaatttggc
    13561 cgccccatag gccaatttca acttacccag aaaaaactgg cggaaatgat caccgagatc
    13621 accaaggccc agttgctgac ctggaggctg ggcgtattgg ccaaccagaa aaaagccagc
    13681 ccggcccaga tctccatggc caagcggaac aatgtgcaca tggcccttac cgtcgcccgg
    13741 gaagcccggc agattttggg cggcatgggc attaccaacg aatatcccat catgcggcat
    13801 atgatgaacc tggagaccgt attgacttat gaaggcaccc acgacatcca tttgctgatc
    13861 acgggtatgg acgtcacggg actgaatgcc ttttcgggtt aaataaataa tgacggcctt
    13921 tcaggcctgc cctttcaggg cgatatgact gacctcatac ccgagcttcg ggcaataggc
    13981 ccgaagctcg ggaaatggcg ccctttcagg gctaactcat acgttgcttt cctgttcctg
    14041 tttgacgcct ccatgcagga attcattttc ggttaaatgg cgccctttca gggcgatacg
    14101 actgacctca tacccgagct tcgggcaata ggcccgaagc tcgggaaatg gcgcnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccttc agggctaagg catacgatgc
    14281 gttcctccat cctggaattt aatctgaggc taaccaatga tctgaaaggg ggtgtctaaa
    14341 aagtcaagat atcgtccagt cagtgtgcgg aatgatcagt acaaacgagg ttgtatcagg
    14401 cgtcctcgcc cgcctgcagt ctggcaggcc tgagactggt tcgcaatatt atagtgccat
    14461 cagaaaagca atatgaaatt attgtttgta aactctctta tgacatactg ctaaaacgct
    14521 tcaggcggga cgcccgcctg accacaactg tatcctattg accagtcggg caggcactga
    14581 agcaactccg ccttgtttgg ccattcatga ggcctgaaag gccgtcattc cccctgcatt
    14641 gggcggctgc ccaatgcagg ggattaaaac agatccattc gccctgaaag ggcatcagtt
    14701 atctacgcaa caaaatccgt ttataaaaaa ttggattgaa aatgagattc acaggagtgg
    14761 aatcagtcgg tgtacaaaat tgaaagtgag tcctccagta ttagccacaa aactcccccc
    14821 tttgacgtga tttgatccgc ttaacgattt gttttatttt gtaactatta tttaactgat
    14881 aaagccggta tttttgcact agaaatgaat aagatacgcc tctggtccgg tatttattcc
    14941 tgtggattgc tgtttgcctt tttctcggcc caggcccagc ccagatccaa taccgattcg
    15001 atccggctgg tcaatccttc atttgaagac atccccaaac aaagttcccc tcccctgggc
    15061 tgggcggact gcggctttac gggcgaatcc gccccggatg tccaccccag caatttctgg
    15121 gaagtcagca aaccggcgat acatggaaaa acctatgtcg ggctggtcgt gaggaataac
    15181 gatacctggg aacggatcag ccagatgctg gaatcccccc tgcaggccgg tcaatgttat
    15241 catttcagca ttttcatttc cacttccgaa aactacaaga gccgcaccaa tgtgggcaac
    15301 aatccgctcc tgcgcatgca gagcgattcc atcttcaatt ttaatatccc ggcagtattg
    15361 cggatctggg cgggtaatat ctcctgcggc cgctccgagt tgctggccga aagcgtaccg
    15421 atcgaaaacg tggaatggaa aagatatgat ttcaggctga agcccaaggc caattacaat
    15481 tatatcatgt ttgaggcctt ttttaaaacc ccggtgcttt ttccttacaa tggaaacatc
    15541 ctgctcgata acgcctcaag cctccagccg gtcccctgta accaacccat ggaaccccct
    15601 aagccggtag ccgtagcccc aaaacccaaa cccccggtct ggcaagccgg aaaggatact
    15661 tccaaaccgc ccccacccgt ggtattcagg cccaaaccca aggtgggcga gatcataaag
    15721 atcaacaatc tctacttcaa agcggacagc gacagcattc cgccgaacag ctaccaggcc
    15781 ctggatgaaa tattccatta cctgcaagac aacgaaagcc tggtcgtgga gatcgggggg
    15841 cataccaacg ggcttcccaa agatgatttc tgcgacagcc tttccgaact ccgggcaaag
    15901 gcggtggccg aatacctcat gcgcaaaggc ataccccggg agcggatgac ctatgtcggc
    15961 tatggccgcc ggaaacccat cgacaccaat gccacccgtg aaggccgggc gcgaaaccag
    16021 cgcgtagaat taaaaatcct gagttcctga tgtccttatt taaaatcgga caggaacgtt
    16081 ccagggaaga aattcaggaa tatctccacg ctactgcctg gatgcccggg gaaaccgtgc
    16141 attccacaag caaggccggg gagggcaata tgaatgtcat cctgagggtc agtttctttt
    16201 ccggaaaaag cctgatcctg aaacaatcca ggccttatgt aaacaaattc ccctccatcc
    16261 ccgctccgcc cgaacggatc gaaacggaaa acgcttttta ccagcttgtg cgcaaacatc
    16321 cggaattatc ggatttcatg ccccgggtga tcggatttga ccggccaaac cggatccttg
    16381 ccctcgaaga cctgggtgaa gggagcgact atacttttat atacaaaaag gattcatcct
    16441 ggccccttga tttaaaccgg gatgcccttc ggtttttatc catcctgcac catcttccgg
    16501 tccccgaagg atacccctcc aacctggccc tgcgccaact gaacgccgaa cacctctttg
    16561 atttccctta ccgcaccgac cacggttttg acctggacaa ggtccagtat ggtctggagg
    16621 acctggcaag accctaccgg gaggatcttt tattgcggga agcgataaaa ggactgaaaa
    16681 aaatatacct tggccagggt ggccgccagt tgcttcacgg cgactatttc cctggcggct
    16741 ggctgcaggc cgggggaggc tttaaggtga tcgaccctga attcagtttt atcgggcctg
    16801 ccgaatacga cctggggatc atgatggctc atctgttcat ggcgcgtcag gataaaaacc
    16861 aggttgcggg attttgcagc gcctacgatt ccccaagcgg tttttctgat accctttgct
    16921 ggcaattttg cgggatggag attttccggc gtctcatcgg cctggcccaa ttgccggtgg
    16981 acctcagcct ggaggaaaaa gcaagcttac taaaacaggc ttatgaatgg gtgacgggat
    17041 cgggtcaggc ttcggggcat tttggggaat ccggggtgtc aaaaccagtg acttcgtagg
    17101 ggcaccctga ctttcaaata actacaacaa tccaaacaaa accgcctggg ccgcaaaaat
    17161 ccggttcctg gcctgcttca gcaccaggga atccggccca tccaatacct catccgcgat
    17221 ttccaggttt cgccgggtag gaaggcagtg catgaatttc ccctgactgg tcaagtccat
    17281 tttttttgaa gtgatcatcc aggatgggtc ctggctcagg attttaccat aatcccggta
    17341 tgcgctccag tttttggcat agacaaagtc tgcgccttcc atcgctttgt cctggtcgta
    17401 ttcgatacgg gcgccaggtg taaaggcctc gtccagttca tatccttccg ggtgggtgat
    17461 caccagatcg tatccgtatc ccagggtcca ttgggaaaaa ctgttggcta ccgcctgggg
    17521 cagggcgcgg atatgcggcg cccaggtgag gaccactttg gtgttttttc tatcctgact
    17581 gcggtgctgc tccatggtca tgatgtccgc aaaggactgc aggggatgca gggtggagga
    17641 ttccaggttg aggatgggca ccctggagta cttgcaaaaa cccgtcatga cttcctcgcg
    17701 gtagtctttg tcccggtcgg tgagcccggg aaaggagcgg atggcgatga ggtccaccat
    17761 ctggctcatg acggccgccg cttccttcac atgttcggcg cggtcggcgt tcatgacgac
    17821 tccctcttca aattccatct tccagccctg gtccgcgttc aggccgatca ccttcatgcc
    17881 gagctggtag gcggcctgtt gggtgctcag ccgggtccgg agactggggt taaaaaacaa
    17941 catgcccagg gtcttgtgcc tgccggcatc ttcggactgc caggggtccc gggcaaagga
    18001 caaagcctgc tcaaggaggg cttccttatc ctccacatcc cggaaagaaa taaaatgatt
    18061 catgcaacgg gcataaatat aaggcaatcg ccgcaaggtc gaattactcc ggtgacgtgt
    18121 aatcaaaaat gtcgtacgtt taaatgggac tgacaaaagg caaaaaatgc ctgaccgcct
    18181 aaagacacaa atagctcaag gatagcgaag cagtaatgtt caaagacatc tatacctact
    18241 tcgtgagcct ggtgtcttct atataactca atgccaaacc attggtaaaa aaagaggact
    18301 tcggtgaaga aatataaaat tcaaatcacg aaggaggctt atgcccatcc ttttaccaaa
    18361 gttatctctc cggtgttgca gtgcaccaaa ctcctgcaca ctttgccgga gatattgagc
    18421 ttcacccaaa catggggcgg gctgtgtcgg gacacactag atcccgtagg gatttcatac
    18481 gggtagcaga aaattttggg atttaatatc gtcccggatg ggacgaaata acctgaccgt
    18541 atatgctacc
//
LOCUS       DAOQXT010000096        18473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3392_length_18473_cov_7.771691, whole genome shotgun sequence.
ACCESSION   DAOQXT010000096 DAOQXT010000000
VERSION     DAOQXT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18473
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3392_length_18473_cov_7.771691"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3077)
                     /locus_tag="PKM27_14875"
     CDS             complement(<1..3077)
                     /locus_tag="PKM27_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNR08601.1"
                     /translation="MMDSIATKRRTTLNKLHLYILQMFLGLAAMMPFKIGAQTNTVVL
                     PSDGNHTKSVAPQGSLRYQRGFYLIQRKEILASGLQSGDTISCIGFTIADAQNDTTRA
                     KFKVYLQNTTDTVSRIDTAWTVIPGIANTYQLVTGLHPGNYEWQVRSDCSPFSPILGF
                     NNDNLDSCRPVTHLKEGNITDVSAELSWVSPAHSVTDFFVEYSLTDTENWIGMSTTNT
                     TITLNGLAPNTGYQWRVKTRCASDSSVISNSSFVTEKSNNCNSPTGLSVVSVGDTTAK
                     LDWGPAVGNTFYSVRFRRVGNTAWVETISFADSISINSGLVPGTTYEWQVRSNCNISD
                     AGAFIAGPRFTTSGPTVCYFPPYFSVDSITSSSVKFSWDSSVAATSYELRYRAKDVIS
                     WTNATNPMSLVHDDSIAVPDTIGPYDIPFKGMNLDTFVYTGQGVYVAWEYEYMTGPLS
                     SPNTSLATLANSVLKGSFGQDSLRTILSFITRSDTSALALDTVLNANDYRPETRLCSP
                     SLKDSVEVLAVYALGKYAPRYTSAPVSAVIRNHSPDTLTYTVNITVKEQLTQTVRYTV
                     NQNITVGPDTIGLIEFSGWSPVLLETDSIIVSVPAQGDENVLYNNRNYYLQTVTPSTV
                     SYEDGSNAISQAGTDTLAGMTLGRHYMDGCGKINAIQVFLSQSAQGHPVYAVALDTNL
                     VILGQSSAIVPDSAQVNKYYTFYLPGTRLLQNEYYYVGLAQDSSMVSCFPVGVQYEGG
                     RIRDSAYFRGNAAGDSIWHHPYPGRLMIRAELIPGTAVPSITGDLFLCTGTSDTLVVS
                     SILARYADSVIAYSSQYSPYQYSAREALGSPNVYPGYGPDPKSWISSSEAGRESLVLH
                     FPEADSVNFVDVFETFNPGALDSIYLQDEGTGMFHLIWSGTAAPAPPSSKKNRFNFPL
                     TPYKVSGVRLAFNMDTVPGYSAIDAVCIGRLVTPGDFTSLNWTGGSTDDTLIISAPGT
                     YTVTTVDAMGCMSVDSVTSISPVSVIPVITVEGPLSFCPGDSVKLKSSQTGGNT"
     gene            complement(3091..5901)
                     /locus_tag="PKM27_14880"
     CDS             complement(3091..5901)
                     /locus_tag="PKM27_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08602.1"
                     /translation="MKKLYFILCFQLLLVAARAQVALKGINYQAAAHNPQGNLLRNQQ
                     INLKIYLFGNENDKRVNHYSEIHQVTTNQQGLFNLVIGEGLRVEGQYGLVPWSTENIW
                     LEVAIQDKGHSGFSSVSNSKLLAVPYALYALTANKLIHSGSASQPAPPEPGVISNQWS
                     VFGNANTDASGNPYHINSLGTTDKVDLFLITDNVERLRIFKGGDISAKLNFEIGQNLK
                     VGQNMQVGLTATVDDSLVVKRSVFFNTDTGATVNYGPFTVAKGSPSLLSGRLTVEQAT
                     DLNAALNVDGPTDLNDRLNVNNMSPTKLTGTLQVDSFTVLNDSLNVSLGSPTAFSGTL
                     EVDSCASFHDKVRILSIYQTDTSGAAPTGSLQVGGGAYIRENLYIGGVAKFGGPVAFG
                     GAVSITDLTQSVNPMTGALRVSGGTGIGLNLNVGGAAGIGGMLTLLDLTESIDSTTGA
                     LKVFGGMSIRRRLNVRGATAFAGSLNIGGISTLNNTMYVRSSGNFIAQFTNTSNQNGI
                     SIQVANAVPEWANNFMEFRNSSAGVVGYIEGENSTQSVNNLLYAHEIENLNFRIHSAE
                     LAIVIATATLFIAGKTLVSALSSSTGCSGLGFCVAAPITSLIVKATLDVGGAAADLIN
                     NSFRTVDAIAEKDAYINYKAARVGVTYESGSGDYAEWLPKSNDGEAFLPGYVVGLRNG
                     FISKQTTDAQKIMVISSQPIVLGNTPPESLRSRYEKVAFLGQVPVRVMGRVHAGDYIL
                     PSGNEDGLARGMAPDEMKAEDYANMVGVAWSAHDQEGYGVVMTAVGLHGSEINKKVVE
                     QKNRIQRLREKVTQRNQVLAQLVPGFQELASGSNSTVTRSVRPGSSPGNSQGQPSLKE
                     FAKSLSYFEFSQEQISEILDQALVMARQKGTQPDEDPFWAKIESDQAYRKSFIEEIQK
                     SARNELPRLFEQNLQQTKVNQK"
     gene            complement(5968..8832)
                     /locus_tag="PKM27_14885"
     CDS             complement(5968..8832)
                     /locus_tag="PKM27_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08603.1"
                     /translation="MKSLRRSTSRLMASLFRYRAQTAKKWYAVFCFQLCVLAFGYAQV
                     IPKGMNYQAVARNPKGELLSHQKIGLKISLVAMDQGQKITHYSETHEVATNALGLFNL
                     VVGEGRKAGGEFGLIPWNAQNIWLEVSIRDPKDSEFVLLSSSKLQFVPYAIHAGLAGG
                     LAGPGNLQPSSNSSAPEPGVESTTWSVFGNHNTDLAGNPYHINALGTTDFVDLILITD
                     NIERMRIRADGNIITKLNFDITKNLKVGQNLTVDLTASVGDSLLVKGNVILNQVSGFT
                     LVKGPYTVENQSPSILTGTLTVDRSVDLKETLNVDGNTDLNARMFVNNRSPSLLTGTL
                     QVDSVTNLNDALTVTNASPTTLTGTLRTDSCATFNDRLKIDSQFSTDTSGISPSGSLQ
                     VGGGTYVKENLYVGGVAKFGGPVAFAGAVTIQDQTQSTSPSTGALKVSGGVGIGLNLN
                     VGEAAMIGGMTSVTDVTESTDTLTGALKVFGGAGIRRRLNVGGAVSLFDSLTVSGLTT
                     MGNALQVTSGSPYIAHFINTSNQNGISIKLANPSPGWSNNFIEFRNSNSGVVGRIEGQ
                     NQSEYLNNPNYIRELDIYKTDLKFAEATVITASYFLVGAAAGVVAAAASSTPCAGLGV
                     CGTTPIVSLIVKAAAELAARSIGLARAVELRDHFQERKDEFVNYHAARIGVTYESGAG
                     DYAEWLPKRDTQETFLPGYIVSLQYGQITKRTEGYGKLLVISTKPAVLGNSPEPSLKR
                     NYEKVAFMGQVPVLVMGKVRSGDYILPSGNQDGLGRAVPVENMQAEDYIQIVGTAWSD
                     KETESCGLVNVAIGLNDSDISKLAEDNQGQIQELKSELDQTHSILARLVPGFQPATRS
                     REAAGQAVNVQNPLAGELWFKEASTGRTDFSQVSRDQVEGYLMMAEKSLRDNGGVPEN
                     YAFWRQYQTDPSFKQNCIASIQGIYKQSVRLQTERIKQNK"
     gene            complement(8829..11618)
                     /locus_tag="PKM27_14890"
     CDS             complement(8829..11618)
                     /locus_tag="PKM27_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08604.1"
                     /translation="MKKLIFILVFQIAFLAAGQAQVIPKGMNYQAVARNLRGEIIADQ
                     PIGLKIFLFSNENDIRTNYYTELHEVKTNALGLFNLIVGEGKKEQGVYGLIPWNRENI
                     WMEVAIQEKGKSGFTTVSSSKLMAVPYAIHAGTADRLTGKNPDPVATYAPPEPGVIST
                     DWSVFGNAKTDASGNIFRLNSLGTTDFVDLIMITDNQERMRIWGYGDIRTKLNFEIGK
                     NLTVGQTLYVVHSASVGDSLIVLKNVYLNTLTGSTINCGPFTVADLSPTLLTGTLAVD
                     QLADFNTTLNVDGPTDLNGRLYVNKKSPTKLSGTLQVDSTTHLNDALQVNNKSPVYLT
                     GTLEVDSAAVFNDKVKITSLFSTDTSGQFPTGSLQVGGGTYIKQNFYVGGIAKFGGPV
                     NFAGAVSITDVTQSTSPSTGALKVSGGVGIGLNLNVGGAAGIGGMFTVKDLTESVDSL
                     TGALKISGGASIRKRLNVRGAGLFYHTLDVAGISSFNGGLNVTNSNPFVASFINTTNQ
                     HGITVQVNNAAPGWSNNFVEFRNSAGAVVGRIEGENSTQFTSNPRYVTELNLLEAYVR
                     AAELTLVEASVNVVLAGGDVVAAATSSTACLGLGACVTFPIPSLIAVAAIRLAARVAV
                     VGGAGYALDLANDRKQDFIDLKAGLYGVTYESGAGDYAEWLPKENPEEIFKPGDIVGV
                     HGGLISKTIRAHSKLMVVSSQPIVLGNMPDADRKGQYEKVAFLGQVPVRVAGKVMAGD
                     YILPSGNQDGTGRACPSSKLKISDYGSIVGTAWSESDGSEERLVNVAIGLQTRELATL
                     VARQEKEIEDLEREFDETNEQLARMVPGFKQAAGITGEKTYQAASTIQEKTDPSYRLN
                     KWLPTELKAPEILESLSMVEKSFLETGGSREAIYLLNQIKTTPDFIEQYLTNFRFTDT
                     QFLRKQLEKIKPRQK"
     gene            complement(11646..12146)
                     /locus_tag="PKM27_14895"
     CDS             complement(11646..12146)
                     /locus_tag="PKM27_14895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08605.1"
                     /translation="MMKFKSRVHMKAILGLILAFSYLQLDGQSVLAAAGGSDTQKQLT
                     LEWTLGEVFVESVSTRENWFTEGFHQPLLSTRPVNSSAGGYDIKIFPNPTQDLLNVLI
                     KTPLSEFIELSLSDVTGKIIFTRKVPSGSPEIQLRVKEVPEGMYLLSVENQQGYRIGS
                     FKVIKL"
     gene            complement(12154..16941)
                     /locus_tag="PKM27_14900"
     CDS             complement(12154..16941)
                     /locus_tag="PKM27_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HNR08606.1"
                     /translation="MQRIRIETWGAQGGGSMKWISERCPDEGPIIQNDGGLGGYAVGE
                     LDVVPGQLLYIYVGGQGRVGTNEENDGGYNGGGDGGRYGGGGGGATDVRTNPFDLDSR
                     VIVAGGGGGGNTGCPDGGIGGPGGGTMIGESDGTSLYVWDPPGGGGTLIAGGAAGSYG
                     EAGSWGFGGGTVADSQFHASGGGGGWYGGGSAYGTGGGGGSSMAGMMGYSSLQNGSTI
                     LGVREGHGLVVISLLEDNVCSFCELSCNSKVNISMPASECYRIMRPEELLSNVTNNCT
                     TFGYGLTISYPFGTSRLHGNDVDRSHLGLGLIFKIETPDASNSCWGYLYMEDKAPPVA
                     GCMGTREVSCYQLNKLLDLSTQVVDNCSSRNEAVLVRLLFADKGCQNDSVIGVLSRTI
                     RATDSWKNTSICSDTLIITRDSIEETQGPDLVPLACKITCKTDPATQGPAQSDYEDIY
                     FSRDSSHPYYPDPEFLLHLQQRDSFLTENHCIPQELKIVPYLYDSVLIWESGQYIKTW
                     QPVDQYPNRSTFCKIQVSYHDQVLPSCGASAFKIRRQWRLTDWCTGEEKSFIQYIEVA
                     DRNPPVLFMENGGLEALDNRLNYNVNTGPHSCLGEVNLKELVAVDCNAAIRQTYRVSF
                     TDNSHPPKITVLEGNLPGKINLPANGAVYGVKCHDIEVTLRDECSNRFDTLIRACIVD
                     ETPPEILVKGALTSTMDPATCWSRIYAKDLDNGSRDHCCDVLHFAIATQDSINAARKY
                     VYDAIIEQCGFADYDSSREYYDFYIEDYISSYIFKDYLDLTACEEYQIVVRVWEACGI
                     PRYDPHIWPCSEHQWFLYNAGYPRSHFRADHNLNFGFSQNANYSKFKAPKDCNWRYPL
                     IFCDPLLAEWFALAGLDDFNPYYEGAGAAELCNFDFYWPRLGLRSAGNTSNTSGNPPG
                     NTCSRMLWKDGMVRVNVVDKTPPLAEKPDDLVWYCDGLLASGHNPYEFARCDDDSYGE
                     NNAKDHSCVDANGKPYNEIECIRENDSDSGDGVDGTGKPFGWYGCNSYGPSHPGEHGK
                     PVPCASGAGTWAPLYCHSWLCLDASDRLEKTDPSLNFWKPRFHSGPLDSLDAGDGYFW
                     IWDNCKIDSTGLVVKDSSFADACGNGWLQRTWFSKDECGNEVTTSQKIITKHRSDFEV
                     LFPPDVITACTDPAALSQDVLGRPMVMDDECEVVGMVYTDELFNDLPDACYKIVRTWR
                     VINWCKFEPNQAVGSGDILVDDRLIADPVYRPCVYRHLKDNGDGLITYTQIIKVRDSF
                     PPVLACRDTTLCILDQECEQPEINIPFQATDNCTEESQIQYRWELDENPSAGDLAAKN
                     YNKNSIEKISLGNTNRLVLDQAAPVSLVHVFAKDHCGNEDTCTYVLRIEDCKKPTPYC
                     YSGLATVVMPTSGRVTVWARDLDAGSYDNCTAKSALTFSFSEDRNEVKKDFSCVDLPD
                     GKSARIPVRIFVWDETGLFDYCNTHLLIQDGIGNSCPDTLSIIKNRKPVGFLVSTGFS
                     PAIPGARIQGSSSPDKESLLQNLPNPFHSSTRIRFVLIRNENYRLRIVDLTGKTIWTR
                     EGWAEAGIHEVMIKESDLKGPGLYYYTLETQGSLITKKMLHTQ"
     gene            complement(17357..18295)
                     /locus_tag="PKM27_14905"
     CDS             complement(17357..18295)
                     /locus_tag="PKM27_14905"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNR08607.1"
                     /translation="MATEKIKAFAPATLSNICVGFDVMGLAIEEPGDEVLASRHASPG
                     AHILSTNHEKIPFDPKLNTASVAVQAMLEKYGDPALGIGLDIIKKMPVGTGMGSSAAS
                     AAAALVAANALLGDPFRKIELLEFAVIGEKAADGATHADNVAPSLLGGLMIIRSLSPV
                     DVVQLPIPGELYIGLLFPHITILTKEARGILRPEVPMKQFIQQTANMGAFVSALYRED
                     WELMGRSLVDLVIEPQRAKLIPFLDEVKKAAYQAGALTCSISGAGPAIFSFAKGKSNI
                     TRVAQAVEEVMNGLNIPYDFHISRINQKGSVVMEGK"
     gene            complement(18285..>18473)
                     /locus_tag="PKM27_14910"
     CDS             complement(18285..>18473)
                     /locus_tag="PKM27_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08608.1"
                     /translation="EVSPDNPFYQLAGSDNMIVFTTERYHERPLVVRGPGAGAEVTAA
                     GVFAEVVTIGDYLSGYGY"
BASE COUNT         4370 a         4841 c         4563 g         4699 t
ORIGIN      
        1 gtattgcccc cggtctggct ggatttcaat ttaacactgt cgccggggca aaagctgagg
       61 gggccttcca cggtaatcac cgggatgacg gataccggag agatcgaagt aacgctgtcg
      121 accgacatac aacccatcgc gtcaactgtt gtaacggtat aggtcccggg tgcagaaatg
      181 attagcgtgt catcggtact gcctcccgtc cagttcaaac tggtaaaatc tcccggcgtc
      241 accaggcggc cgatacatac cgcatctatt gcgctgtatc ccggcaccgt gtccatatta
      301 aaagccaggc gcaccccgga tactttataa ggggtcaacg gaaaattaaa ccggtttttt
      361 ttggaggacg ggggagccgg agcggcggtt cctgaccaga tcaggtggaa catgcccgtc
      421 ccttcgtcct gcagatagat actgtccaag gctcccgggt tgaaagtttc gaagacatcg
      481 acaaaattta cggagtctgc ttccgggaag tgaaggacca aagattccct gccggcttcg
      541 ctggaggaaa tccaggattt ggggtcggga ccataacccg gatagacatt ggggctaccc
      601 agggcttccc gggcgctgta ttgatacgga gagtactggg agctgtacgc gatcacgctg
      661 tccgcataac gggccaggat actggatacg acaagtgtgt ccgaagtgcc ggtacacaaa
      721 aacaaatcgc cggtaatact aggtaccgcc gtgcccggaa taagttctgc ccggatcatt
      781 aaacgtcccg gataaggatg atgccatatg ctatccccgg cagcgttgcc tctgaaataa
      841 gcactgtccc ggatccggcc cccttcatac tggactccga ccggaaaaca ggaaaccatg
      901 ctggaatcct gggcaagccc tacataataa tattcatttt gcaataggcg ggtacccggc
      961 agataaaaag tataatattt attgacctgg gccgaatccg gtacgatagc ggaagattga
     1021 ccaagaatca ccaggttggt atccagcgcc acggcataaa ccggatggcc ctgggcggac
     1081 tggctcaaaa agacctgtat ggcattgatc ttgccgcatc cgtccatgta atgacggcct
     1141 agcgtcatgc cggccagggt atcggtgccg gcctgggaga ttgcattcga accgtcttca
     1201 taggaaacgg tggaaggcgt tacggtctgg agataataat tccgattatt gtagagcacg
     1261 ttttcatcac cctgggccgg taccgagacg atgatggaat cggtctccag aagaaccggt
     1321 gaccatccgc taaactcgat taaaccgatg gtgtcgggcc ccaccgtaat attttgattc
     1381 acggtgtagc ggaccgtctg ggtcagttgt tccttcaccg taatatttac cgtataagtc
     1441 agggtatccg ggctatggtt ccggatcact gcggacacgg gtgccgaagt ataccgcggg
     1501 gcgtatttcc caagagcata caccgcaagg acctccacgc tgtctttcaa ggaaggcgaa
     1561 cacaaccggg tttccggccg gtaatcgttt gcattcaaaa cggtatccag cgccagggcc
     1621 gaagtgtcgc ttcgagtgat aaatgacagg atcgtgcgga gggaatcctg gccaaaactc
     1681 cctttcagca cagaattggc aagggttgcc agcgaggtgt tcggggagga aaggggaccg
     1741 gtcatgtact cgtattccca ggcgacataa accccctgtc cggtataaac gaaggtgtcc
     1801 aggttcatgc ctttaaacgg aatatcatag ggtccgatgg tatcggggac cgcgatgctg
     1861 tcgtcgtgca ccaggctcat gggattggtt gcattggtcc aggagatgac gtcctttgcc
     1921 cggtacctca attcataaga ggtggcagcc acggaagaat cccaggaaaa tttcaccgaa
     1981 gaagaagtaa tgctgtccac ggaaaaataa ggcggaaaat aacaaaccgt gggcccggag
     2041 gtggtaaacc gggggccggc gataaaagcc cctgcatcgg agatgttgca attagatctg
     2101 acctgccatt cataggtcgt tcccggtacc aacccggaat tgatactgat cgagtccgca
     2161 aatgaaatgg tctccaccca ggcagtgttg cccactcttc taaaccggac ggagtaaaaa
     2221 gtgttgccta ccgccggacc ccagtccaat ttcgccgtgg tatctccaac actcactacc
     2281 gaaagaccgg taggggagtt acagttgttg gatttttcgg tgacaaagga tgaattgctg
     2341 atcacggagg agtccgaggc gcagcgggtt tttaccctcc attggtaacc cgtattggga
     2401 gccaggccgt tgagggtgat ggtggtattg gtcgtgctca ttccaatcca gttttccgta
     2461 tccgtcaggg aatattcaac aaaaaaatcg gtcacggaat gggccgggga cacccaactc
     2521 aattcagcgc tgacatcggt tatgttgcct tctttcaaat gggttaccgg ccggcaggaa
     2581 tccaggttat cgttattaaa accaaggatg ggtgaaaagg gggaacaatc ggacctgacc
     2641 tgccattcat aattgccggg atgcagtccg gtgaccagtt ggtaagtatt ggcaatgccc
     2701 ggaatgaccg tccaggccgt gtcgatccgg ctgacggtgt ccgtggtgtt ttgaagatag
     2761 accttaaatt tggcgcgggt ggtatcgttt tgggcatctg caatcgtaaa tccgatgcag
     2821 gagatggtat ccccggactg aaggccgctg gccagtattt cctttctttg aatcaggtaa
     2881 aagccccttt gataacgaag tgaaccttgc ggagccacgg atttggtgtg attgccatcg
     2941 gaaggcaaga ccactgtatt cgtctgcgcc ccgattttga acggcatcat tgcagccagg
     3001 cccagaaaca tctgcaaaat ataaagatga agcttattca atgttgtccg tctcttggtg
     3061 gcaatggaat ccatcatgga taatgaaggg ttatttttga ttgaccttgg tctgttgcaa
     3121 attttgttca aaaagtctcg gtaattcgtt tctggcgctc ttctggatct cttctataaa
     3181 ggatttccgg tatgcctggt ccgattcaat ttttgcccag aaaggatctt cgtccggctg
     3241 agttcctttt tgcctggcca tgaccagggc ttggtccagt atctctgaaa tttgttcctg
     3301 tgaaaattca aaataggaca ggctttttgc aaattctttt aaggatggtt ggccttggct
     3361 gtttcccggg gatgagccgg gccggacaga ccgggttacc gtggaattgg agcccgaggc
     3421 cagttcctga aagcccggta ccaattgtgc aagaacctga tttctttggg taaccttttc
     3481 acggagtcgc tgaatcctgt ttttttgttc caccactttt ttattgatct cgctgccgtg
     3541 gaggcctact gcggtcatga ccactccgta accttcctga tcgtgagcgg accaggccac
     3601 ccccaccata ttggcataat cttctgcttt catctcgtcc ggggccattc ccctcgccaa
     3661 tccatcttca ttcccggaag gcaggatata gtcccccgca tggacccgcc ccatcacccg
     3721 gacaggtacc tgtccgagaa aagctacctt ttcataccgg gaccgcaggc tttccggcgg
     3781 agtatttccc aagactatag gttgggatga aatgaccatg attttttgcg catcggtggt
     3841 ttgtttggaa atgaacccat ttctcaatcc gactacatat ccgggcaaaa aagcctcgcc
     3901 gtcgtttgac ttgggcagcc attccgcata atccccagaa cccgattcgt aggtaacccc
     3961 taccctggcc gctttataat tgatataagc atctttttct gcaatggcgt caacggtccg
     4021 gaaactgttg ttgatcaaat cggctgccgc acccccaaca tccagtgttg ccttaacaat
     4081 cagggaggta atcggggcgg cgacacaaaa gcccaggccg gagcagccgg ttgaggagga
     4141 taaggcgctg accagggttt taccggcaat aaataaagta gccgtggcaa tgactatcgc
     4201 aagttccgca ctgtggatcc tgaaattcag attttcaatt tcatgggcat acagcaggtt
     4261 gttgacagac tgggtactgt tttccccttc aatatatcct accacaccgg ccgagctgtt
     4321 tcggaattcc ataaaattat tggcccactc gggtactgca ttggccacct ggatgctgat
     4381 tccattttga ttgctggtat tggtgaattg ggcaataaaa ttgcctgaac tcctgacata
     4441 catggtattg ttcagagtgg aaatcccccc aatgttcagc gaaccggcaa aagcagtcgc
     4501 tcctcttaca tttaatcgcc tgcggataga catgccccca aaaaccttta acgcgcccgt
     4561 cgtggaatca atggactccg taaggtccaa tagggtcagc atcccaccta ttccggcagc
     4621 tccacctacg ttgagattaa gaccgatccc ggttcccccg gagactctta aagcgccggt
     4681 catggggttg accgattgag ttaaatccgt gatcgacacc gccccgccga aagcaacagg
     4741 tcctccaaac ttggccacac ccccgatata taaattttcc ctgatgtagg cgcctccccc
     4801 tacctgcaaa gaaccggtag gcgctgcccc ggaggtatcg gtctggtata tggataaaat
     4861 cctgacctta tcatgaaatg aggcacagga gtccacttcc agggtgccgg aaaacgcggt
     4921 ggggctgcct aggctgacgt tcagggagtc attcaatacc gtaaaggaat cgacctgcag
     4981 agtacccgtg agtttggtcg ggctcatatt atttacgttt aaccggtcgt tcaggtcagt
     5041 gggcccatcc acattgagcg ccgcattcaa atcggtggcc tgctccacgg taagcctgcc
     5101 tgacaacagg gacgggctgc ccttggcaac cgtgaaaggg ccgtaattga ccgtggcacc
     5161 ggtatccgta ttaaagaaaa cacttctttt taccacaagg gaatcgtcca ctgtcgcggt
     5221 cagtcccacc tgcatattct gcccgacctt cagattttgc ccgatttcaa aattgagttt
     5281 ggccgagatg tccccaccct tgaaaatcct caaccgttcg acattgtccg tgatcaaaaa
     5341 taaatccact ttatccgtgg tgccgagtga attgatatgg tatggattac cgctggcatc
     5401 cgtattggca tttccgaaca ccgaccattg gttggaaatc acaccgggtt ccgggggcgc
     5461 aggctgggaa gcgctgccgg aatggatcag tttatttgcg gtcagcgcgt acagggcgta
     5521 aggcacggca agcagtttgc tgttgctcac ggaggagaaa ccggaatgcc ctttgtcctg
     5581 gatggcaact tccagccaaa tgttttctgt gctccagggc accagcccat attggccttc
     5641 gacccggagt ccttccccta taaccagatt aaacaagccc tgttggttgg tcgtcacctg
     5701 gtgtatttcg ctgtaatggt tgaccctttt atcgttttca ttgccgaata agtaaatttt
     5761 caaatttatt tgttgattgc gcaacaggtt tccctgggga ttgtgcgccg cagcctggta
     5821 attgattcct ttgagggcca cctgggcccg ggctgcaacc agcagcagtt gaaaacacaa
     5881 aatgaaatac agttttttca tttcaatttc cttttcgaat aaaaaaatcc acggtcgttt
     5941 tccgggtcat tggatatatc catggtttca tttgttttgt ttgattcgtt ccgtttgaag
     6001 tcgcaccgac tgtttataga tcccctggat ggaagcaata caattttgct taaaggaagg
     6061 atcggtttga tattgtctcc agaaggcata attttccgga actccaccgt tgtcgcggag
     6121 ggatttttct gccatcatga ggtatccctc cacctggtcc ctggaaacct gggaaaaatc
     6181 ggttcttccg gtgctggctt ctttgaacca aagttctccg gctaaaggat tctgcacatt
     6241 caccgcctgg cctgcagctt ccctgctgcg ggttgcgggc tgaaagccgg ggaccagtct
     6301 ggccaggatg gaatgggtct gatccagttc ggatttcagt tcttggatct gtccctggtt
     6361 gtcttcggcc aatttactga tatcggaatc attcaatccg atggcgacgt tgaccaatcc
     6421 gcaactttct gtttccttat ccgaccaggc cgtgcctacg atctgaatat aatcttcagc
     6481 ctgcatgttt tccaccggca cagctcttcc taatccgtcc tgattacccg agggcaggat
     6541 ataatccccg gacctgactt tacccattac caaaacaggt acctggccca taaaggcgac
     6601 tttttcataa ttccttttaa gggatggttc agggctgttt cccaatacag cgggtttggt
     6661 ggagatgacc agtagtttgc cgtaaccctc tgttcttttt gtgatttgcc cgtattgcag
     6721 actcacgata tatccgggga ggaaggtttc ctgggtatcc cgtttgggca accattccgc
     6781 ataatccccg gctcccgatt cataagtcac cccgatccgg gccgcatggt aattgacaaa
     6841 ttcatctttc ctttcctgga aatgatctct caattccaca gccctggcca aaccaatgga
     6901 acgagccgca agttctgcgg cggctttcac aatcaaagag acgatcggtg tggtaccgca
     6961 taccccgagt cctgcgcagg gggtggagga ggccgcagcc gcaaccactc ccgcggcggc
     7021 accgaccaaa aaataggaag ccgttataac ggtggcttcg gcaaatttta aatccgtctt
     7081 gtaaatatcc agctcccgta tataattggg attgttgagg tattcggatt ggttctggcc
     7141 ctctattctc cccactactc ctgaattgct gttgcggaat tcaataaaat tattggacca
     7201 gcccgggctg gggttggcta gtttgatgct gattccattc tgattgctgg tattgataaa
     7261 atgggcgatg taaggcgagc ccgaggttac ctgcaaagca ttgcccatgg tcgtaagccc
     7321 cgacaccgtc agcgaatcaa acaaggatac ggcaccgcca acgttgagcc ttcgccggat
     7381 cccggctccg ccgaaaactt ttaaagctcc cgtaagcgta tctgtggact cggtgacatc
     7441 ggtcaccgaa gtcattcctc cgatcatggc ggcttcccca acatttaaat tcaagcctat
     7501 gcccaccccc ccggacactt tcaacgctcc ggtggatggg ctggtggatt gggtttggtc
     7561 ctgtatggtt acggccccgg caaaagcaac cggtccgcca aatttggcga cacctccgac
     7621 atacaggttt tctttcacat aagtccctcc gcctacctgt aaggagccgc tggggcttat
     7681 tccggaagta tccgttgaaa attgggagtc aattttaagc ctgtcgttga aggtggcgca
     7741 ggaatccgtt ctcagggtac cggtaagagt ggtgggactc gcgttggtta cggtaagcgc
     7801 atcattcagg ttggtcacgg agtccacctg cagggttccg gtcagaaggc ttgggctcct
     7861 gttgtttacg aacatcctgg cgttgagatc cgtatttcca tccacattca gtgtttcctt
     7921 gaggtccacc gaccggtcga ctgtgagggt tccggtcaga atgcttggac tttgattctc
     7981 caccgtgtag ggacctttaa ccagggtaaa tccactcacc tgattgagaa taacattccc
     8041 tttgaccaaa agcgaatccc cgacggatgc cgttaaatcc accgtcaggt tttggcctac
     8101 cttaaggttt ttagtaatgt cgaaattgag tttggttatg atgtttccgt ccgccctgat
     8161 ccgcatcctt tctatgttat cggtgatcag gatgagatca acaaaatcgg tcgtgcccag
     8221 ggcattgata tgataggggt tgccggcgag atctgtattg tgattgccga ataccgacca
     8281 ggtagtagat tcgacgccgg gttctggagc ggatgaattt gaggacggct gtaagttgcc
     8341 cggccccgcc aggccgcccg ccaggcctgc atgaatggca taaggaacga attgcagttt
     8401 gctgctgctg agcaggacaa attctgaatc cttagggtcc cggatggata cttccaacca
     8461 gatgttctgg gcattccatg gaataagtcc gaattcgccc ccggccttcc tgccttcccc
     8521 gacaaccaaa ttaaacaaac ccaatgcatt ggtggccacc tcatgggtct cactgtaatg
     8581 cgttatcttc tgtccctgat ccatggccac cagggaaatt tttaacccga ttttttgatg
     8641 actcagcaat tcacctttgg gattgcgcgc cacggcctga taattcattc ctttgggaat
     8701 gacctgggca tagccgaagg ccagcacgca aagctgaaaa caaaaaacag cgtaccattt
     8761 tttagccgtt tgcgccctgt atcgaaaaag ggatgccatc aaccgggatg tacttcgtcg
     8821 aagggatttc atttttgacg cggtttaatt ttttccaatt gtttacgcaa aaactgggta
     8881 tcggtaaacc ggaaatttgt caggtattgt tcgataaaat ccggggtggt ttttatttga
     8941 ttcaacaggt aaatggcctc cctgcttcca ccggtttcca aaaaactttt ttccaccatg
     9001 ctcagggatt ccagaatttc gggggccttt aattccgttg gaagccattt atttaaacgg
     9061 taggaaggat cagtcttttc ctggatagtc gatgcggcct ggtaagtttt ttctccggtg
     9121 atgcccgccg cctgtttaaa cccggggacc atccttgcaa gttgttcatt ggtctcatca
     9181 aattcccttt ccaggtcttc gatttccttt tcctggcggg caacaagggt tgccaattca
     9241 cgggtctgaa gtccaatggc cacgttgacc aaccgttctt cactgccgtc ggattccgac
     9301 caggccgtac cgacaatgga tccgtaatcg cttattttca gttttgagga cggacaagcc
     9361 ctgcccgttc catcctggtt gccggacgga aggatataat cccccgccat caccttaccg
     9421 gccactcgta ccggaacctg acccaggaag gccacttttt cgtactgtcc tttgcggtct
     9481 gcatccggca tattccccag gacgatgggc tggctggaaa caaccatgag tttgctgtgt
     9541 gcccgtatgg tttttgatat caggcctccg tggacaccca caatgtcacc cggtttgaaa
     9601 atttcctcag ggttttcttt aggcaaccat tcggcataat ctccggctcc ggattcatag
     9661 gttacaccgt ataagcctgc tttaaggtct ataaaatctt gctttcgatc atttgccaga
     9721 tccagggcat aacccgcacc gcccaccact gctacgcgag ccgccaaacg aatagcggct
     9781 acggcaatca gactggggat gggaaaggta acgcaggcgc ccagacccaa acaggctgtg
     9841 gaagaggtgg ccgctgccac cacatcccct cccgcgagaa caacgttgac gctggcttcc
     9901 accaaagtca gttctgctgc cctcacataa gcttccagca aattcagttc agtgacatac
     9961 cggggattgc tggtaaactg ggtggagttt tctccttcaa tgcggccgac caccgctcct
    10021 gcgctgtttc tgaattccac aaaattattg gaccagcccg gagccgcatt attgacttgt
    10081 acggttatgc catgttgatt ggtggtattg ataaatgaag cgacaaacgg gttggagttg
    10141 gttacattca gacccccgtt aaaactggaa attccggcaa catcgagcgt atggtaaaac
    10201 aaacccgctc cccggacatt cagccttttc ctgatgctcg cacctccgga aatctttaat
    10261 gctcccgtca gcgaatccac ggattcggtc aggtctttaa cggtaaacat cccaccaatg
    10321 ccggctgctc cccccacatt gaggtttaat ccaataccga ctcccccgga aaccttcaat
    10381 gccccggtgg atggactggt tgattgcgtg acatctgtga tggaaaccgc ccccgcaaaa
    10441 ttcaccggtc ctccgaattt ggcgatgccc ccgacataaa aattctgctt gatataagtg
    10501 cctccgccta cctgcaagga acccgtcgga aactgtcccg aagtatccgt ggaaaacaag
    10561 gaggtaatct tgactttgtc gttgaatacc gctgcagaat ccacttccaa tgtgccggta
    10621 agataaaccg gactcttgtt attgacctgc aaggcgtcgt tcagatgggt ggtggaatcc
    10681 acctgcaaag tcccgcttaa cttggtgggg ctttttttat tgacatagag tctcccgttt
    10741 aaatccgtag ggccatccac gttcagggta gtattgaagt ccgccaactg gtccacggcc
    10801 agcgttccgg tcaataaagt gggactgaga tcggctacgg taaagggtcc gcaatttatc
    10861 gtggacccgg tcagggtatt gaggtaaaca ttttttaaga caatcaggct gtcccctacg
    10921 gaggcgctgt gaaccacata gagggtttga ccgacggtga ggtttttgcc tatttcaaaa
    10981 ttcagcttag ttcggatatc cccataaccc cagatcctca ttctttcctg gttgtcggtg
    11041 atcatgatga gatctacaaa atcagtggtg cccagtgagt ttaatctgaa gatgttgccg
    11101 ctcgcatccg ttttggcgtt tccgaataca gaccaatccg tggaaatcac accgggctcc
    11161 gggggtgcat aagtggcgac cgggtccggg tttttaccgg tcagccggtc tgcggtgcct
    11221 gcgtgaatgg catagggtac ggccatcagt ttgctgctgc ttaccgtggt aaaaccggat
    11281 ttcccttttt cctgtatggc cacctccatc catatatttt ctctgttcca tggaatcagt
    11341 ccgtatactc cctgttcttt tttcccctct ccgacaatca ggttgaataa tcccagggca
    11401 ttggtcttca cttcatgaag ctcagtataa taattggtcc ggatgtcgtt ttcgttgctg
    11461 aacaaaaata ttttcaaacc aatgggttga tcagcaatga tttctccccg gaggtttctc
    11521 gctacggctt gataattcat gcccttggga atcacctgag cctgccctgc ggccaggaag
    11581 gcgatttgaa aaaccaggat aaaaattaac tttttcatgt ggagtaaaat cgggaaatca
    11641 aaaagctata gtttaatcac tttaaatgaa ccgatccggt atccttgttg gttttccacg
    11701 gaaagcaaat acatgccttc cggcacctct tttaccctga gctgtatttc cgggctgcct
    11761 gaaggaactt tccgggtaaa gattattttt ccagtgacgt ccgaaaggct cagttcaatg
    11821 aattccgaca atggggtttt gatcaataca ttcagcaagt cctgcgtagg gttcggaaaa
    11881 atcttaatat cgtatccacc ggccgatgaa ttaaccgggc gggtggacaa caggggttga
    11941 tgaaatcctt ccgtaaacca attttcccgg gtgcttacgc tttccacaaa tacctcaccc
    12001 agggtccatt ccagggtcag ctgtttttgg gtatctgatc cgccggctgc agccagcacc
    12061 gactgtccgt cgagctgcag gtatgagaag gcgaggatca aacccaaaat cgctttcata
    12121 tgcaccctgc tcttgaattt catcatggtt tcattattga gtgtggagca tttttttagt
    12181 gatcagggat ccctgggttt ccagcgtata ataataaaga ccagggccct ttaaatcgga
    12241 ttccttaatc atgacttcgt ggattcccgc ctcagcccat ccttcccggg tccatattgt
    12301 tttaccggtc aaatcaacaa tccgtaaccg gtaattttca ttccggatca acacaaaccg
    12361 gatccttgtg cttgaatgaa aggggttggg cagattttga agcaaagatt ccttatccgg
    12421 tgaggaggat ccctggatcc gggcacccgg aatggccggg ctgaacccgg tagataccag
    12481 aaatccaacc ggtttcctgt ttttaataat ggacagggta tccggacagc tatttccaat
    12541 cccatcctga atcaataaat gggtattgca ataatcgaag aggccggtct catcccatac
    12601 aaaaatccgg accgggatcc gggccgattt gccatcgggc aggtcaacac aactgaaatc
    12661 ttttttgacc tcattgcggt cctcgctgaa actgaaagtc agggctgatt ttgcagtaca
    12721 attatcataa cttcctgcat ccagatccct ggcccatacc gtgacccttc ccgatgtggg
    12781 catgactacc gttgcaaggc ctgaataaca ataaggagtg ggttttttac aatcttcgat
    12841 ccttaataca taggtgcagg tgtcctcgtt cccgcaatgg tctttggcga agacgtgcac
    12901 cagggatacg ggggccgcct ggtccagcac caaacggttg gtgtttccaa ggcttatttt
    12961 ctcaatgctg tttttattgt aattttttgc ggccaggtct cccgccgatg gattttcatc
    13021 cagttcccaa cgatattgaa tctggctctc ctcggtacag ttgtccgttg cctggaaagg
    13081 aatattgatc tccggctgtt cgcactcctg gtctaaaata caaagggtag tatcccggca
    13141 ggctaagact ggaggaaagc tgtccctgac cttgatgatc tgggtatagg tgatcaggcc
    13201 gtccccgttg tctttgaggt gccggtaaac acagggtcta tatacggggt ccgcaatcag
    13261 gcggtcatcg accaggatat cgcctgaccc gacagcttga ttaggctcaa atttgcacca
    13321 gttaatgacc cgccaggtcc ggacaatctt ataacaggca tccggcaggt cgttaaacag
    13381 ttcatctgta taaaccattc cgaccacttc acattcgtcg tccatcacca tgggcctgcc
    13441 caacacatcc tgggaaaggg cggccggatc cgtacaagcc gtgatcacat ccgggggaaa
    13501 aagaacctca aaatcagaac ggtgtttggt gatgatcttc tgggaggtgg tgacttcatt
    13561 gccacattcg tctttggaaa accaggtccg ttggagccat ccgtttccgc aggcatctgc
    13621 aaaggaggaa tccttcacca cgaggccggt actgtctatc ttgcaattgt cccagatcca
    13681 aaagtaacca tccccggcat ccagggaatc caaaggcccg ctatggaacc ggggcttcca
    13741 gaaattcagg cttgggtctg ttttctccaa tcggtccgaa gcatccaggc acaaccaact
    13801 gtgacaatac agcggtgccc aggtcccggc acccgaagca caaggaacgg gtttcccatg
    13861 ttcacccgga tggctgggcc catagctgtt gcatccatac cagccaaaag gttttcctgt
    13921 accatctacc ccgtcccccg aatcggagtc gttttcccgg atgcattcta tttcattgta
    13981 aggtttcccg ttggcgtcca cacaactatg gtctttggca ttattctcgc cataactgtc
    14041 gtcgtcacag cgggcaaatt cgtaagggtt atgtccggaa gccaggagcc catcgcaata
    14101 ccagaccagg tcatccggtt tttctgcaag gggtggggtt ttatccacca cgtttacccg
    14161 gaccatcccg tctttccata acattctgga acaggtgttt cccggtggat ttccagaagt
    14221 gtttgatgtg tttccggccg acctcagccc caggcggggc cagtaaaaat caaaattgca
    14281 caattcggcg gcgccggccc cttcgtaata aggattaaag tcgtccagtc cggccagggc
    14341 aaaccattcc gccaggagcg gatcacagaa gatgagcggg tatctccagt tgcaatcctt
    14401 tggagcttta aattttgaat aattggcatt ttgagaaaaa ccaaagttca ggttatggtc
    14461 tgccctgaaa tggcttcttg gatatcctgc gttatataaa aaccactgat gttccgaaca
    14521 gggccagata tgcgggtcat agcggggaat accacaagct tcccataccc tgaccacgat
    14581 ctggtattct tcacaggccg tgaggtccag gtaatccttg aaaatgtatg aactgatata
    14641 gtcctcaata taaaagtcat aatattccct ggaagaatcg taatcggcga agccgcattg
    14701 ttcgatgatg gcatcatata cgtatttgcg cgcggcattt atggaatcct gggtggcaat
    14761 ggcaaaatgc agaacatcac aacaatgatc ccggctgccg ttgtccaggt ctttggcata
    14821 aatcctggac cagcaggtgg ccgggtccat ggtggaggtc agggcgccct tgaccaggat
    14881 ttcaggaggg gtttcatcca cgatgcaagc cctgatcagg gtatcaaacc ggttggaaca
    14941 ttcatcgcgg agcgttactt caatatcgtg gcattttaca ccgtagaccg ccccgttggc
    15001 cggcaggttt attttaccgg gcagattgcc ttccagcacc gtaatttttg ggggatggga
    15061 gttgtcggta aaactgaccc ggtaagtttg ccggatggcc gcattacaat ccactgcaac
    15121 caactcttta aggtttactt ctcctaaaca ggaatggggt ccggtattta cattataatt
    15181 taaccggtta tcgagcgcct ccaggccacc gttttccata aagagaacgg ggggattccg
    15241 gtcggcaact tctatgtatt ggataaagga tttttcttcc cccgtgcacc aatccgtcaa
    15301 tctccattgc cggcggattt taaaagcgga ggctccgcaa gacggcaaga cctggtcgtg
    15361 ataggacacc tggattttac aaaaggtact tcggtttggg tattgatcca ccggttgcca
    15421 ggttttgatg tattggccac tttcccagat caggacgctg tcgtagagat aagggactat
    15481 tttcaattct tgcgggatgc aatggttttc cgtcaggaaa ctgtcccgct gctgcaggtg
    15541 gagcaggaat tcagggtccg gataataagg gtgagaggag tcccgggaaa aataaatatc
    15601 ctcataatcc gattgggccg gtccctgggt ggcaggatct gtcttgcagg taattttgca
    15661 agccagggga accagatcag gcccctgggt ttcttcaata gaatccctgg taatgatcag
    15721 ggtatccgag cagatggaag tatttttcca ggaatcggta gcccggatgg ttctggaaag
    15781 gactccgatt accgagtcgt tctgacaacc tttgtctgca aacagcaatc tgaccagtac
    15841 ggcttcattc cggctggagc aattatccac cacctgggtg ctcaaatcca gtaatttatt
    15901 aagttgatag caggaaacct cccgcgtccc catgcagccc gccaccggag gcgctttgtc
    15961 ctccatataa agataacccc agcaagaatt ggatgcgtcc ggggtttcta tcttaaagat
    16021 aagcccaagc cccaggtgtg accggtccac atcgttgccg tgcaacctgg aggtgccaaa
    16081 gggataactg atggtcagcc cataaccaaa ggtggtacaa ttgtttgtga cgttactcaa
    16141 taattcttcc ggccgcatga tgcggtaaca ttccgaagcg ggcatggaaa tatttacttt
    16201 ggaattgcag gataattcac aaaaactgca aacattatct tccagcaggg agatcaccac
    16261 cagtccatgg ccttcccgca ctcccagtat ggtacttcca ttttgcaagg aactgtaacc
    16321 catcattccg gccatggaag atcccccgcc tccaccggtt ccataagcac tcccaccgcc
    16381 ataccatccg cctccgcctc cgcttgcatg aaattgggaa tcagcaactg ttcctccgcc
    16441 aaatccccaa ctgccggcct ctccatatga acccgcggct cctcctgcga tcaaggtgcc
    16501 ccctcctccg ggtgggtccc aaacatataa ggaggtgccg tcactttctc caatcatggt
    16561 tccgcctccc ggtccaccaa taccgccatc cgggcagccg gtattgcctc cgcctcctcc
    16621 gccggcaacg atcacccggg agtccaggtc aaatggattt gttcttacat cggtcgctcc
    16681 gccgcctccg ccaccatacc tgccgccatc gcctccgccg ttgtatccac cgtcattttc
    16741 ttcattggtc ccgacccggc cctgcccgcc tacatatata tataataact ggccggggac
    16801 tacatccagc tctccaaccg catacccccc gaggcccccg tcattttgga tgattgggcc
    16861 ctcatcggga catctttccg agatccattt catcgaacct cccccctggg ccccccaggt
    16921 ctcaattctg attctttgca cacaactggg cacataccag ggctgaaccc taccggtata
    16981 ttggaattct atatgctgcg cctggagcgg aaactgaaaa agaaaggtga aaaaaatcaa
    17041 acatgcaatc caaccggcca atgggcaact aaaggacaaa gctatgcctg ttctgatttt
    17101 ttgggtaata aacttcatca gcttggagcg gactagaacc taaatgtaga attaaatccc
    17161 cataccccat aacatactga aaagatataa attatatata acactttcta atgtataagg
    17221 gtttgcctta tattttttta aacaaataag aaatatataa tttgtttaat taaaattcac
    17281 ccgttcagga tatcccgttc attttattcc ctaatgagag gaagggtcat agaggaacag
    17341 attttcaaat tgcctttcat ttgccttcca tcaccacgct tcccttttgg ttgatccggg
    17401 aaatatgaaa gtcgtatggg atattcaatc cattcatcac ttcttcgacc gcctgggcga
    17461 cccgggtgat attggatttg ccttttgcaa aactaaaaat ggccgggccg gcacctgaaa
    17521 tggaacaggt aagtgccccc gcctgataag ccgctttttt tacttcatcc aggaaaggga
    17581 tcagcttcgc cctttgcggt tcaataacca ggtccaccag gctgcgaccc atcaattccc
    17641 agtcctcccg gtataaagcc gaaacaaagg cccccatatt ggcggtttgc tggataaact
    17701 gtttcatggg cacttcggga cgcaaaatgc cgcgcgcttc cttggtgaga atggtgatgt
    17761 gcgggaacaa caatccaata tacagttctc ccggtatggg caattggacc acatcgaccg
    17821 gactgaggct ccggatgatc atcaggcccc ccagcaaaga gggggctaca ttgtccgcat
    17881 gggttgcgcc gtccgccgct ttttccccaa tcactgcaaa ttccaggagt tcgattttgc
    17941 ggaaaggatc accaagcagg gcattggccg ccaccagggc tgcggctgcg ctggcggcac
    18001 tggatcccat tcctgtgccg accggcattt ttttaatgat atccagtcca attcccaggg
    18061 caggatcacc gtatttttcc agcatggcct gtacggccac cgaagcggtg ttcaattttg
    18121 gatcaaaggg gatcttctca tggttggtgg aaagaatatg ggcgccggga ctggcatgcc
    18181 tgcttgccag cacctcgtcc ccgggttctt caatggccag gcccattaca tcaaatccaa
    18241 cacagatatt ggacagggtc gccggtgcga acgcttttat tttttcagta gccatatccc
    18301 gacaaataat ctccgatggt cacgacttcg gcaaaaaccc cggccgccgt cacttcagcg
    18361 cctgcacccg gtccccgcac caccaggggg cgctcatggt acctctcggt ggtaaagacg
    18421 atcatgttat cgcttcccgc caattgataa aagggattgt cgggggatac ctc
//
LOCUS       DAOQXT010000097        18262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3455_length_18262_cov_5.804361, whole genome shotgun sequence.
ACCESSION   DAOQXT010000097 DAOQXT010000000
VERSION     DAOQXT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18262
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3455_length_18262_cov_5.804361"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..526)
                     /locus_tag="PKM27_14915"
     CDS             complement(185..526)
                     /locus_tag="PKM27_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08609.1"
                     /translation="MKISRKKFVLGFLILAFAFQFITNSVLGPEVRLFPANGEWFPGT
                     ESPMNWKNKLATILYPVKYILIKPLSFLGKDPDAPPPVLIFAFAMYWSAIALFLHFLL
                     SLLFRRKKTGV"
     gene            complement(873..3050)
                     /locus_tag="PKM27_14920"
     CDS             complement(873..3050)
                     /locus_tag="PKM27_14920"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HNR08610.1"
                     /translation="MAKTIIISNRLPVKMEILPGGEIRFKASEGGLATGLGSVYKQGD
                     NIWIGWPGHFLNSVDEQDHVIRELTKENMRPVFLTADEIRLFYEGFSNEVLWPNFHYF
                     PQLVVYEDSYWESYEKVNQKFCDAVLQYTEEGDTIWVHDYQLLLLPQMLREKLPRIAI
                     GYFNHIPFPSFEMFRMLPWRKSLLMGLLGADLIGFHTYDDMRHFLSSVSRLTPFSVKE
                     QFVQRGDRRIKVESFPMGIDYERYADSAAAPETISQEVEYRTALGGKRLILSIDRLDY
                     TKGIPQRLRAFNEFLIRYPEFKEKVSLVMVVVPSRDQVGMYKDLKETVDELVGKINGT
                     HGNMKWTPVHYFYRSIPMQKLSAYYRIADVGLVTPLRDGMNLVAKEFIASKLDKKGVL
                     ILSEMAGAAKELSDAILINPFNKNEFVQAMYQALTMPLPEQRSRIEKMQVSLKKYNIH
                     HWVRIFMDGLAEIKQEQQAYLTKQIDETDLPEILSRFEKAPRRFIFLDYDGTLVGFNV
                     NPAACQPDEELLSIFEGLAKDSRNHVIIISGRPHQTLDAWMGKFPFTLIAEHGVWTKK
                     ENGEWSIKENLRKDWKVEIKKIMDAHVDRTPGSFIEEKNFGLVWHYRGVEVGLGELRS
                     RELMSHLRYLASSRGLQVLEGDKVVEVKNIEVNKGIAAFRFISKNPEDLHLAIGDDWT
                     DEDTFSVMPDEAITIKVGHAASKAKFHLDTVGDVREFLKRLMV"
     gene            complement(3094..4140)
                     /gene="corA"
                     /locus_tag="PKM27_14925"
     CDS             complement(3094..4140)
                     /gene="corA"
                     /locus_tag="PKM27_14925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNR08611.1"
                     /translation="MPRHKRKTGMPPGTPVFTGDKFPVKTFTTCIKYNDSSYEELDDF
                     NLVRLDAEDDFNYWIDIRGVHDSKYIEAVGHKFQIHALALEDILEIEQRPKMDEYQNA
                     IFITAIALKMDAQSGELSKEQITLYFGKNFLISFQEDADDFLKVIRDRIHQKRGRLRQ
                     RKTDYLAYSILDFTIDHYYDALDRIEKKLSDMDELLHEENSIISQKELHDIKIQVIRI
                     RKSIYPMREVINRFIRSENPLIFPETDIYLRDLADHSIQIAEMSETYQDMANSLHDLY
                     QAETSNRLNNVMKILTIISTIFIPLTFIAGIYGMNFHFMPELSWRLGYFLILGVMAAV
                     AILFLLYFRRKKWL"
     gene            4303..5757
                     /locus_tag="PKM27_14930"
     CDS             4303..5757
                     /locus_tag="PKM27_14930"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNR08612.1"
                     /translation="MKPTVFSQFLIAVLAAALVFGAFQMIKGPSKNVIIEHVDGTPAK
                     NTVFTLNENGEAVPLDFTGVAEKSLGAVVHILSVSGTSGRPSQEDLLREFFGQRRSPR
                     VGSGSGVIINPDGYIVTNNHVIQGADDLEVTLHDKRTYKATLVGTDPQTDIAVIKIEE
                     KGLQSLHFTNSDQVRIGQWVLAIGNPFNLNSTVTAGIVSAKGRSIEIFDRSVGGIESF
                     IQTDAAINPGNSGGALIDLNGSLIGINTAIASQTGTYSGYGFAVPANLASRVVSDLIQ
                     YGSPQRGYLGVQIQNVDGNLARQQNLKITEGAFIASVMENGAAQKAGLKANDVVVSIN
                     DKKVTSSSDLMEIVGSHRIGDKLKLKVNRDGNLKDFEVELKNQAGTTAAVTAPKGNGS
                     FEEELGASLSPIDAATAQRMGIRGGVQVNNIGDGLIRDSNIEEGFIITRVNRQPVSQP
                     EEVIAFLQNNTDGVLIEGYYPGQPEKQYYAIRPK"
     gene            5990..6541
                     /locus_tag="PKM27_14935"
     CDS             5990..6541
                     /locus_tag="PKM27_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNR08613.1"
                     /translation="MQTRLSRFSILALLLTLWACNPKTSQMLYTVSPESKLKELGIEL
                     PVPEAPVANYVNAVRTGNLLYTAGKGPNRPDGSQVKGKVGKDLSIEEGYEAARLTAIQ
                     LIAVLKQELGDLSKVKRVVKVLGMVNAEGDFTQQPAVINGCSDLLVKVFGDRGKHARS
                     AVGMGSLPGNIAVEIELIVEVQD"
     gene            6628..8124
                     /locus_tag="PKM27_14940"
     CDS             6628..8124
                     /locus_tag="PKM27_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08614.1"
                     /translation="MRPFLIDIHVHPGLNPYGKSFSTLRGSNSMDPTQENSIWRKDHP
                     NDLERLLERSLGLVKFTQSDFQSLTNGHVHCICASLYSIEQGLVRLKTGSGWLTDTLT
                     NFISGLGKDRINFLQSNKDYFSDLEGEYRFYNELHNRTVWINGTARKYILLKNFTELE
                     TAIASEGHADGLHTIFVILTVEGMHNLDSRLDQAEGDENLFLANVDKLKSWTHPPFFV
                     TFAHHFYNKLCGHAESLVDMIQDQLTRQEYQMNTGFTPLGWKVLDKLLDTANGRRIYI
                     DIKHMSRLSRRQYFEYLQNTRNAEWAAGKLPVIVSHGACNGFKSESEKIHSPGLEDTG
                     RHMYVKDINFYDDEILLVARSGGIFGLQLDERRIANRNYKRGSRLRSLFASKFKRQLA
                     NSRMVWNNIQHIGMLLDQHDLPAWDCMTIGSDYDGLIDSVNLFWSAEDLPALAKCLEI
                     KAEEWLSGGGYAFRNPSNRIGAQEIVDKIFYSNAMEFFRKYFDGTVEI"
     gene            complement(8212..9690)
                     /locus_tag="PKM27_14945"
     CDS             complement(8212..9690)
                     /locus_tag="PKM27_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08615.1"
                     /translation="MRFGLFILLLSFCSCRRAPESEPTDVSYRVETIRLPEGLTGETG
                     AIEFLPDGRLVACFLRGEVMIYDPKKEDWKVFASGLHEPLGLLALSESEMLVMQRPEL
                     TRLVDTDRDGKADLFETVTDDFGMTGNYHEWNYGPVRDTAGNLYIGLNTASEYGIVMP
                     EVRGTLDTTLVPGKPLQKFAAVPYRGWIMQLKPDGSIHPFASGFRSPNGLVIDDLNRL
                     FATDNQGDWVGTSVLYEVKEGHFYGHPASLLWKEGWGRGDPSKLPVRELEAMKTPGSV
                     LFPHGIMAASPTQPVIDRTSGKFGPFAGQFFIGEMNQERILRVMLEEIDGQLQGACIP
                     FLDGQGLIKGSNRMAFAPDGSLWVGHAQHGFAGDLGIQRMVYTGRAPMEVHAMRLQKD
                     GFELTFTQAIHDTVSLNSALFRLRHYYYEYHLKYGSDQFDVQPVPVNRIDISSDRKKL
                     VLVLPEIKAGYIYELTLPGLVSDAGTGLENKLICYTVNRLVE"
     gene            complement(9687..10799)
                     /locus_tag="PKM27_14950"
     CDS             complement(9687..10799)
                     /locus_tag="PKM27_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNR08616.1"
                     /translation="MNNYFKGGYFSAVLLPAFLIIRFIPLSGQVPADTLRIQIHAVSG
                     MQYDLVRFAVNPGSWVKLVLANTDDMEHNLVIGRKGSREKIVAAALALGKDGPAAAYI
                     PIVEEVLHSIQVLKPGEADSMVFRAPRSTGVYPYVCTFPGHGNIMYGAMHVTNGVMPR
                     LAADLHVPEHRRKSEEYHHLKQRPSGHPYTLVPPYHYRVLMPDAGPAAIAVCLSKDLA
                     YCWDAGACRLRYAWEGEFLDLTDYWTIKGELHAKILGTVFYRDQSPFPLRLGHPDSIP
                     AVKFKGYRLIRGYPEFNYLLNGTEIYEWITPVEKGVGLVRQFRIREAGRPVWFVHGSG
                     DGVVYQSDKGQWAENQLKLDPKEAAQFTLTMIKTGQ"
     gene            complement(10800..11984)
                     /locus_tag="PKM27_14955"
     CDS             complement(10800..11984)
                     /locus_tag="PKM27_14955"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HNR08617.1"
                     /translation="MKDKSTTQKLNRRSFHKSIAAGLLGPGLISPGLIGPGLLGPLTA
                     LTDKPAKAESKINHFHHVGGDYHAVMGEPGWNWNDKQKNWTNPRNFQYHQRHGVRHLT
                     NIMEGAWDLDQMKKWKDDCDRGNMVWEAIRMDSGFIYAKPGGDREKKLQEVTENIRKA
                     SLVGVQVVTMHWTMIPIRRNGHVDGRGGSSYHSFKLEDNWRELPAGSRGIIGYEEYWE
                     RITYFLQNIIPVCAEHQLRLGVHPYDPPGLPRGYQGVDNWNAGPGTVFDSLKKYESIV
                     DSPYNGFQLCLGTIMEGLKNPKEELLPIVKYFADKGKICQIHMRNIKGGLHDFQEVYI
                     DEGEADFIEVIRILRDSGYTWSICPDHVPSHPDDPGGYQAFSQAFGYIQGMIDSANTE
                     VR"
     gene            complement(12443..13387)
                     /locus_tag="PKM27_14960"
     CDS             complement(12443..13387)
                     /locus_tag="PKM27_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR08618.1"
                     /translation="MNMEQADRITIPFFKVKGVLLFLFLVNNFYLNAQSRPVQALAPG
                     VFYYFGDELQKKSANCLWVVFKDYVLAVDANYPWGAEEILREIRKTTDKPVRFVFNTH
                     YHHDHTWGNSVFVDSGAVVVASRVTDGEMRTLGQVEWDRRSAYSGGDMGPYRREFPSL
                     TFDRRMVFDDGTHRVELILMGPAHTAGDAVAWLPKEKILATGDLCVNGNPWGNNVADK
                     NADYDRWLRVLDSLASWGASTVIPGHGDPGGSGILKQQRDYLADMLAQVRKGIETGKS
                     KEDLVKEIDLSKHPVRGGNTVSNHRSIRAMYDKLKGLW"
     gene            complement(13420..14016)
                     /locus_tag="PKM27_14965"
     CDS             complement(13420..14016)
                     /locus_tag="PKM27_14965"
                     /inference="COORDINATES: protein motif:HMM:NF015553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter"
                     /protein_id="HNR08619.1"
                     /translation="MDLILTGILTFASTNIDDLFILSMWFGNKRYMPLEIIVGQYLGI
                     LALTGLSLALSLVGLWMDPAYLGLLGLFPIYLGLKSLWRLFRKKEQEVKIDFAEPMQN
                     NMGNVLAVAGVTIANGGDNTGVYVPLFAAMGWEGKGMTVGVFIIMTFLWCLAAKYLAG
                     HPIISKAADKYGHILAPLILIVLGLYILAESGAFGILN"
     gene            complement(14007..14519)
                     /locus_tag="PKM27_14970"
     CDS             complement(14007..14519)
                     /locus_tag="PKM27_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNR08620.1"
                     /translation="MRESRRQLTLFISDQNGMIEKIRAAFNPEQFNLIPAHITLCRED
                     EIEPLEKVLKNLGSIILPGPLRIEFGPVERFEDGKGVLLPGLIGNPGFDDLRKAVLTG
                     IIDIPRNHRPHITLMHPRNSCCTDEIFRQIRVVALPIVLNFDTVSLIEQEQWGPWKVV
                     EHFPICRPWT"
     gene            complement(14607..16307)
                     /locus_tag="PKM27_14975"
     CDS             complement(14607..16307)
                     /locus_tag="PKM27_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR08621.1"
                     /translation="MKRCVQIHPFWFCLSFILFFSCKEKNKIPSLESIGLINLKRGEV
                     TWCGPPDGRFGTVEFETSCSVGGGKDFNLAMALLHSFEYDEAEKVFAKIIDQSPDCAM
                     AYWGVAMSNYHPLWAPPSEAELEKGSRALAVARGLERIPRRESDYLEALSLFYEDWQN
                     TDHRTRSMRFEKAMEKLYYTWPDDIEAAIYYSLALVAAADPADKTFTRQKKAHSILTS
                     LYPNEPDHPGIVHYIIHSCDYPELAELALPAARKYMSIAPSSAHALHMPSHIFTRLGL
                     WDECIQSNLVSAASAKCYAENAGLKGHWDEELHAMDYLVYAYLQKGENDLARQQLEYL
                     NTIHEVSPTNFKVAYAFAAIPSRFVLENRLWNEAATLQLPKINFLWQDYPWQTAIVHF
                     TRLLGNVHLGHLDSARLELAALEKIYDALMAKKETYNANQVLIQINSAKAWIQLQEGK
                     NSEALRLMDMAAQMEDNTEKHPVTPGEVIPARELLGDMLLQVNQPGKALEAYEINLKR
                     HPNRFNSLYGAGLAAAKSNNPEKAQSFYDLLTSVANPNRSERPELEKARSFLKRSDIK
                     "
     gene            16497..17885
                     /locus_tag="PKM27_14980"
     CDS             16497..17885
                     /locus_tag="PKM27_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNR08622.1"
                     /translation="MLRINQELTLNDLKAKISAFWDLSGGKIRALEKEYDPSKGSPVF
                     TAAGKYTTRGWTEWTQGFQFGSALLQYDATGEDWFHNIGRDRTLGFMAPHISHVGVHD
                     HGFNNVSTYGNLLRLMQEGKTPYNEWEKNFYELALKISGAVQASRWTKIKNGGFIHSF
                     NGPHSLFVDTLRSCRALILSHLLGHELQAENDIRISLLDRAFQHIRATADYSVFYGQG
                     RDSYDTWGRTAHECIFNTRDGNYRCPNSQQGFSGFTTWTRGLAWAMCGFSEQLELLKE
                     LSSDEILIAGERELIEAMILKAARATCDFYIQNTTTDGIPYWDTGAPGLVKMGDYLSR
                     PAEPYNDHEPVDSSAAAIGCQGLLRFGLYLTGKGDEELGTHYWQAGLTVLNTLLDQPY
                     LSLDAGHQGLLLHSIYHRPNGWDYVPAGSKIPCGESSMWGDYHIREVALYVQKLLRQE
                     PYYAFFKAVKKD"
     gene            17887..>18262
                     /locus_tag="PKM27_14985"
     CDS             17887..>18262
                     /locus_tag="PKM27_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNR08623.1"
                     /translation="MSRLTDYSRLCIHTITTKPWSIEEACGNFSDAGVKGITVWRNAL
                     EGKALMATGRMIRDRGLQIVSLCRGGFFPSIDAVKRSLALEDNRKAIDEAHALGAPMI
                     VLVCGADPGQPLQESRKQIRDGI"
BASE COUNT         4495 a         4760 c         4555 g         4452 t
ORIGIN      
        1 gacccataac cttgactaca ccagccggga aaaaactcgc ggccaatgca atacgacaaa
       61 atgcggcaaa cccctgaaac catttgtctg actttgcttc ataatatact tcctcaatcc
      121 gattcattta tttgatgttt aaccaaaact ttaattgact ttcggctact attgaagatt
      181 gcacttaaac tcctgttttt ttacgcctga acagcaagct gagtagaaaa tgaaggaata
      241 aagcaatggc actccagtac atagcaaatg caaatatcaa tacggggggt ggagcatctg
      301 gatcttttcc taaaaaagac agcggcttaa tcaaaatata tttgacgggg tataatatgg
      361 ttgcaagctt gtttttccag ttcattggtg attcagttcc aggaaaccat tctccatttg
      421 caggaaagag tcgaacctca ggccctaaaa ctgaattggt gatgaattga aaagcaaacg
      481 ccaaaatgag gaaacccagg acaaactttt ttctcgaaat cttcatagga ttaactttaa
      541 aagaaaaaag aactttgaat tacaaagtaa attaattatt tttatttttc aaagtagtta
      601 aaggaaaaat tttggctgtg gaatggtgcc ctgaaaatgg catggtttca ggaagttcga
      661 acgtacaaca tcaggattgc cgcaacgctg gcggacaatt ggtagtggta cgattaaatg
      721 ctatcccggg tttgagcgat ttttttgctt tttagttgtt aactggaaca tcagtttttc
      781 aaatcggcat aagttgccgg gcggattgta ataaaataca agccatgcgg aaagcttcgg
      841 actgttggcg gtcagtggac aaattacttc tcctaaacca tcagcctttt caaaaactcc
      901 cgcacatccc ccaccgtatc caggtggaat tttgctttgg aagcggcatg tcccactttg
      961 attgtgatgg cctcgtcggg catcactgaa aaagtgtcct cgtcggtcca gtcgtcgccg
     1021 atggccaggt gtaagtcttc cgggtttttg ctgataaagc ggaaagcggc aatgcctttg
     1081 ttcacttcaa tattcttgac ttccaccacc ttgtcccctt ccaggacttg caggccccgg
     1141 gaggaagcca ggtaacggag gtgggacatt agctcgcggg acctcagttc cccgagcccc
     1201 acttccaccc cgcggtaatg ccagaccagg ccgaagtttt tttcttcgat aaaggaccct
     1261 ggggtccggt ccacatgggc gtccatgatc tttttgatct cgaccttcca atccttgcgc
     1321 aggttttcct tgatggacca ttccccgttt tcctttttgg tccagactcc gtgttccgcg
     1381 atgagggtga atggaaattt tcccatccag gcgtccaggg tctgatgggg acggccgctg
     1441 atgatgatga catggttccg ggagtctttg gccagtcctt caaagatcga caggagttcc
     1501 tcatcaggct ggcaggcggc cggattgacg ttgaacccca caagggtacc gtcgtaatcg
     1561 agaaaaataa acctccgggg agctttttcg aaccgggaca ggatctccgg gagatcggtc
     1621 tcatcgattt gtttggtgag ataagcctgc tgttcctgct tgatctcggc aaggccatcc
     1681 ataaaaatcc ggacccagtg atggatattg tattttttga gggaaacctg cattttttca
     1741 atccggcttc tttgttcagg caagggcatg gtcagggcct ggtacatggc ctggacaaat
     1801 tcattcttgt tgaaaggatt gatcaggatg gcgtccgaca attctttggc cgcgccggcc
     1861 atctcggaca ggatcaatac tcctttttta tccagtttag aagcgatgaa ttccttggcc
     1921 accaggttca ttccgtccct gagcggggtg accaatccca catcggcgat ccggtaataa
     1981 gcggaaagtt tttgcatggg gatcgagcgg tagaaataat gcaccggcgt ccatttcata
     2041 ttgccgtgag tcccgttgat ctttcctacc agttcgtcca ccgtctcctt aaggtcttta
     2101 tacatgccga cctggtccct cgaaggcact acgaccatga ccagcgagac cttttccttg
     2161 aactcggggt atcggatcaa aaattcatta aaagccctga gccgttgagg gatccccttg
     2221 gtataatcga ggcggtcgat gctcaggatc agccttttcc ctcccagggc ggtccggtac
     2281 tccacttcct ggctgatggt ctccggagct gcggcggagt cggcatagcg ttcgtaatcg
     2341 atgcccatgg ggaaggattc cacttttatc ctccggtccc ccctttgcac aaactgttcc
     2401 tttacactga aaggcgtaag acggctcacc gaacttaaaa aatgcctcat gtcgtcatag
     2461 gtgtggaagc cgatcaggtc ggcccccagc aaacccatca gcagggactt gcgccagggc
     2521 agcatccgga acatctcaaa ggagggaaaa gggatgtggt tgaaataacc gatcgctatc
     2581 ctgggcagtt tttctctcag catttgggga agcaggagca actggtaatc gtggacccag
     2641 atcgtatccc cttcttcggt atactggagc accgcatcgc agaacttctg gtttaccttt
     2701 tcataggact cccagtaact gtcctcgtag acgaccaatt gcgggaaata atgaaaattg
     2761 ggccagagaa cctcattgga aaaaccctca taaaacaacc tgatttcgtc ggcggtgagg
     2821 aaaaccggcc tcatgttttc tttcgtcaat tcccggatta catgatcctg ttcgtccaca
     2881 ctgttcagga aatgaccggg ccagcctatc cagatattat cgccctgttt atacaccgat
     2941 ccgaggccgg tggccagtcc gccttcgctg gctttaaacc tgatttcacc acccggtagg
     3001 atttccattt tgacaggcag cctgttgctg atgatgatgg ttttagccat agaaaaagat
     3061 gcgtttgaat ttggatttat tggttcgtgc cggtcacaac catttctttc gcctaaaata
     3121 cagcaaaaat aaaatagcca cggccgccat gacacccaag atcaaaaaat aacccaggcg
     3181 ccagctcagt tctggcataa aatggaaatt catgccatag atgccggcga taaacgtcag
     3241 cggaatgaag atggtggaga tgatggtcag gatcttcatc acgttgttga ggcggttgct
     3301 ggtctccgcc tggtaaaggt catggaggga attggccata tcctggtagg tttccgacat
     3361 ttccgcgatc tggatgctgt ggtcggccag gtcccggagg tagatgtcgg tctccgggaa
     3421 aatcaggggg ttttccgaac ggataaaccg gttgatgacc tcccgcatgg gatagatgga
     3481 tttgcggatc cggatgacct ggatcttgat gtcgtgcaat tccttctggg aaatgatgga
     3541 attttcttcg tgcagcaatt cgtccatgtc ggaaagtttt ttctcgatcc gatccagggc
     3601 gtcgtaatag tggtcgatgg tgaagtcgag gatgctgtag gcgaggtaat cggttttccg
     3661 ctgcctcagc ctcccccttt tctgatggat ccggtcgcgg atgactttta aaaaatcatc
     3721 ggcatcttcc tggaaggaga tcaggaaatt ttttccaaaa tagagagtga tctgttcctt
     3781 gctgagctcc ccgctctggg catccatttt cagggcgatc gcggtgatga aaatggcatt
     3841 ctggtattca tccattttcg gcctttgttc gatttccagg atgtcctcca gggccagggc
     3901 atggatttga aatttatggc ctaccgcctc gatgtatttt gaatcgtgga cgccgcggat
     3961 gtcgatccag taattaaaat cgtcttccgc gtccaggcgg acgaggttaa aatcgtcaag
     4021 ctcttcataa gacgaatcgt tgtatttgat gcaggtggtg aaggtcttca ccggaaattt
     4081 atcccccgta aataccgggg ttccgggcgg cataccggtt tttcgtttat ggcgcggcat
     4141 gagcaatgat aaggaattaa atcatagccc ggttcacggt cgttaaaaaa aacaaaaacc
     4201 cgttaaactc cattaaaacc gcccggggat cgggattgtc ccctgccctg cctgtacttt
     4261 tgaggggtaa acctattgat gtccaaaatt gttctttaat ccatgaaacc tactgttttt
     4321 agccagttct taatagccgt tttggctgcg gcacttgtat ttggcgcctt ccagatgatc
     4381 aaggggccgt ccaaaaatgt gatcattgag cacgtggacg gcactcccgc caaaaacacc
     4441 gtgtttaccc tcaacgaaaa cggtgaggcc gttccgctcg attttaccgg ggtggccgaa
     4501 aaatcactcg gggcggtcgt gcacattctt tcggtcagcg gtacctcagg caggcccagc
     4561 caggaagacc ttctccgcga attctttggc caaaggagat cccccagggt cgggagcgga
     4621 agcggcgtca tcatcaatcc cgacgggtat atcgtaacca ataaccatgt gatccaggga
     4681 gcggatgacc tggaagtcac cctgcacgat aaaagaactt acaaagccac cctggtgggc
     4741 acggatcccc aaaccgatat tgccgtgatc aaaatcgaag aaaagggttt acaatccctc
     4801 catttcacca attccgacca ggtgaggatc gggcaatggg tactggccat aggcaatcct
     4861 tttaacctga actcgacggt gacggccggt attgtcagcg caaaaggacg gagcatcgag
     4921 atctttgacc gaagcgtcgg gggcatcgaa tcctttatcc agacagatgc cgccatcaac
     4981 ccgggaaaca gcggcggcgc cctgatcgat ctcaatggca gcctgatcgg catcaatacg
     5041 gcgatcgcca gccaaaccgg gacttattcc ggttacggtt ttgccgttcc cgcgaacctg
     5101 gcaagccggg tggtttccga cctgatccag tatggaagcc cgcagcgcgg ttatctcggg
     5161 gtgcagatcc agaatgtcga tggcaacctg gccagacaac aaaatctgaa gatcacggaa
     5221 ggcgctttca tcgcttcggt gatggaaaac ggcgcggccc agaaagcagg cctcaaagcc
     5281 aacgacgtgg tggtttccat caatgataaa aaggtgacca gcagttccga cctgatggaa
     5341 atcgtgggca gccaccggat tggggataaa ctcaaactta aagtgaaccg ggacggcaac
     5401 ctcaaagact ttgaagtgga attaaaaaac caggcaggga ccaccgcggc cgtgacggcg
     5461 ccaaaaggca acggctcctt tgaggaagag cttggcgcct ccctctcccc catcgatgcg
     5521 gctaccgccc agcgcatggg catccggggc ggcgttcagg taaataatat tggcgacggt
     5581 ttgattcggg acagcaatat cgaagaaggt tttatcatca cccgggtcaa ccgccagccg
     5641 gtcagccagc cggaggaagt catcgccttc ctccaaaaca atactgacgg ggtcctgatc
     5701 gagggttatt atcccggcca gcccgaaaaa cagtattatg ccataaggcc aaaatgagtt
     5761 tttagatttg agtaaatatt tttctggaat gggtcggcgt ccgctggccc attttttttg
     5821 gtggatggtc agaggtggat ggtcagaggt ggatggtgtt gggtggttgc tcctggcgtc
     5881 ctcgcctgga gctatacaag tttcaggcgg ggacgcctga aacaacaatt ccacaattcc
     5941 acaattccac aattctacaa tacgctatct tggtaccaaa tccaaaacca tgcaaacccg
     6001 attgtcccgc ttctccatcc tggcccttct ccttaccttg tgggcctgta atcctaaaac
     6061 cagccagatg ctgtataccg tcagtccaga aagcaaactc aaagaattgg gcatcgaatt
     6121 gccggtgccg gaggctcctg tcgccaatta tgtcaatgcc gtacgcacgg gcaacctgct
     6181 gtacaccgcc ggaaaggggc ccaaccggcc ggacggcagc caggtaaaag gcaaggtggg
     6241 caaagacctc agtattgaag aaggatatga agccgcccgg ctcactgcca ttcaactgat
     6301 cgcggtgctg aaacaagaac tgggcgacct gtcgaaggta aaacgggtgg ttaaagtcct
     6361 cggaatggtc aatgccgaag gggattttac ccaacaaccc gccgtgatca acggctgttc
     6421 cgacctccta gtaaaggtct ttggcgaccg gggcaaacac gcccggagcg cggtaggcat
     6481 ggggtccctg cccggcaata tcgccgtaga gatcgaactc atcgtggaag tccaggacta
     6541 aacctttgcc tatatttaag gcagggaggc tgaccaaatg cactcctttt acactttatg
     6601 gtattgataa ccttttaaag tgctgccatg aggccttttc tgatcgatat ccatgtccac
     6661 ccgggtttga atccctatgg aaaaagtttt tcaacactgc gagggtccaa cagtatggat
     6721 cccacccagg aaaacagcat ctggcgaaag gaccacccca atgacctcga gcggctcctg
     6781 gagcgcagcc tgggcctggt caaatttact cagagcgact ttcaaagcct caccaacggc
     6841 catgttcatt gcatctgcgc ctccctgtac tccatcgaac agggactggt gaggttaaaa
     6901 accggttcgg ggtggttgac cgataccctc accaatttta tctcgggact gggtaaagac
     6961 cggatcaatt ttctgcagtc caacaaggat tatttcagcg acctggaagg ggaataccgt
     7021 ttttacaatg agctccacaa ccggacggtg tggatcaacg gcaccgcgag gaaatatatc
     7081 ctccttaaaa actttaccga gctggagacg gccatagcct ctgaaggcca tgctgatggt
     7141 ttgcacacca ttttcgtcat ccttaccgta gagggcatgc acaacctgga ttccaggctg
     7201 gaccaggcgg aaggcgatga aaatctcttc cttgccaatg tggacaaact caaatcctgg
     7261 acccatcctc ccttttttgt gactttcgcc catcattttt acaataaact ttgcgggcat
     7321 gccgaaagcc tggtcgatat gatccaggac cagctgaccc ggcaggaata ccagatgaac
     7381 accggcttca caccacttgg ctggaaggtc cttgataaac tgctcgacac tgccaatggc
     7441 cggcgcattt atattgatat caaacacatg agccgcctgt cccgccggca atatttcgaa
     7501 tacctgcaaa atacccgcaa cgcggaatgg gcagccggaa aattgccggt catcgtaagt
     7561 cacggcgcct gcaatggatt caaaagcgaa tcggagaaga tacacagccc aggtctggag
     7621 gacaccggcc gccacatgta tgtcaaagac atcaactttt acgatgacga aatcctcctg
     7681 gtggcccgca gcggcggcat tttcggactg cagctggatg aaagaaggat tgccaaccgg
     7741 aactacaaac gcggttcccg tctccggagc ctttttgcat cgaagttcaa aaggcaactg
     7801 gccaacagcc ggatggtatg gaacaatatc cagcatatag gcatgcttct tgaccaacac
     7861 gaccttcccg cctgggactg catgaccata ggcagcgatt acgacggcct gatcgattcg
     7921 gtgaatttat tctggtccgc ggaagacctt cccgccctgg cgaaatgcct ggagatcaaa
     7981 gcggaggaat ggctgtccgg agggggttat gcttttagaa atccctccaa ccggataggg
     8041 gcacaggaga tcgtcgacaa gattttttat tccaatgcca tggaattttt caggaagtat
     8101 tttgatggga cggtggagat atagatcagc agatatgcca aattttgaaa atttggcata
     8161 tctgaataat gcctaatcct gctggatatt ttgcggcagc attgccagta gctactctac
     8221 caacctattc accgtataac aaatcaattt attctctaga cccgtccctg catcagaaac
     8281 caaaccgggg agggtgagtt cataaatata accggccttg atttcaggca atactaaaac
     8341 cagtttcttc cggtcggagg aaatatcgat ccggttcacc ggcaccggtt gcacatcgaa
     8401 ctggtctgaa ccatatttca aatgatattc ataataataa tgcctcaggc ggaacagcgc
     8461 cgaattcaga gaaaccgtat cgtgtatggc ttgggtaaaa gttaattcaa atccatcctt
     8521 ttgcaagcgc atcgcgtgga cttccattgg agccctcccc gtgtacacca tgcgctggat
     8581 ccccaggtcg cccgcaaaac catgctgggc atggcctacc caaaggctgc cgtccggtgc
     8641 aaaggccatg cggttgctgc ccttgatcag cccttgtccg tccaggaaag gaatacaggc
     8701 accctgcaac tggccatcta tttcttcaag catcaccctt aaaatgcgtt cctggttcat
     8761 ctcccctata aaaaactggc cggcgaaggg gccgaatttc ccggaggtcc ggtctatcac
     8821 cggctgagtg ggcgaagcgg ccatgatccc atgcggaaac agcaccgaac ccggggtctt
     8881 catggcctcc agttctctga caggcaattt ggaagggtct ccccggcccc acccttcttt
     8941 ccataaaagg ctggcgggat gaccgtaaaa atgcccctct ttgacctcgt ataagacgct
     9001 ggtgcccacc cagtccccct ggttgtccgt ggcaaacaaa cggttgagat catcgatcac
     9061 caggccattc ggcgaacgga agccgctggc aaaaggatgt atggagccat ccggtttcaa
     9121 ttgcatgatc catccccggt atggcacggc ggcaaatttc tgaagcggtt ttccgggcac
     9181 caaggtcgta tccagcgtcc ccctcacttc aggcatgaca atgccatact cggaggccgt
     9241 gttcagcccg atgtatagat ttccggccgt atcgcgaacc ggcccgtaat tccattcgtg
     9301 gtaattgccc gtcatgccaa agtcatcggt cacggtttcg aagaggtccg cctttccatc
     9361 cctatccgta tccaccaacc gggtgagttc cggacgttgc atgacgagca tctcgctttc
     9421 actgagggcc aggagcccca ggggctcgtg caatccggat gcaaaaacct tccagtcttc
     9481 ttttttggga tcgtagatca tcacttctcc ccttagaaaa caagccacca aacggccgtc
     9541 cggcaaaaat tcaatggccc ccgtctcacc ggtaaggccc tcaggcaggc ggatcgtttc
     9601 gacccggtag gaaacatcgg ttggctcgga ttcaggcgcc ctgcgacagg agcaaaagga
     9661 aagtagcagt ataaataacc caaacctcat tgtccggttt ttatcatggt gagggtaaat
     9721 tgggcagcct cttttggatc gagctttaat tggttttcgg cccattgtcc tttatcgctc
     9781 tggtaaacca ctccatcccc ggatccgtgg acaaaccaaa ccggccgccc tgcttccctg
     9841 atacgaaatt gccggaccag gcctaccccc ttttccactg gggtgatcca ttcataaatt
     9901 tcggtcccat taagcagata attaaattcg ggataacccc ttatgaggcg ataaccctta
     9961 aattttacgg ccgggatgga atcgggatgt cccagccgca aaggaaaggg gctttggtcc
    10021 ctgtaaaata ccgtgccgag aatttttgca tgcagttcac ctttgatggt ccagtagtcc
    10081 gtgaggtcca gaaactcacc ctcccaggca tatcgcagcc tgcaagcgcc ggcatcccaa
    10141 caataggcca ggtccttcga taaacaaaca gcaatggcag cggggcccgc atccggcatc
    10201 agcacccggt aatgataagg ggggaccagg gtatagggat gcccggaagg cctttgtttt
    10261 agatggtggt actcttcgct ttttctccgg tgctccggca catgaagatc cgccgccaaa
    10321 cgcggcatca ctccattggt cacatgcatg gccccgtaca tgatgtttcc atggccgggg
    10381 aaagtgcata cataaggata aacgcccgtg gaccggggcg cccggaaaac catggaatcg
    10441 gcctcgcccg gttttaggac ctgaatggaa tggagcactt cctccactat tgggatatag
    10501 gccgctgccg gaccgtcttt gcccaaagcg agggcggcag ctacaatctt ttcacggctg
    10561 cctttccgtc cgatcacgag attgtgctcc atatcgtcgg tattggccag caccagtttg
    10621 acccagcttc ccgggttaac tgcaaaacgc accaggtcgt attgcatccc ggatacggca
    10681 tggatctgga tgcgcagggt atccgccggg acctgaccgg ataggggtat gaaacgaatg
    10741 ataaggaagg caggcaataa aaccgccgaa aagtaacccc ctttgaaata attattcatc
    10801 taccggactt ccgtattcgc tgaatcaatc atcccctgga tgtaaccgaa agcctgggaa
    10861 aaggcctggt atcctcccgg gtcatccgga tggctgggta catggtccgg gcagatcgac
    10921 caggtatagc cggaatcccg gaggatccgg atcacttcaa taaaatccgc ctcgccttca
    10981 tcgatataca cttcctgaaa atcgtgcaaa ccgcctttga tattgcgcat gtggatctgg
    11041 cagatctttc ccttatcggc gaaatatttg acgatgggca gaagttcctc tttgggattc
    11101 ttcagtcctt ccatgatcgt tcccaggcaa agttgaaaac cgttgtaggg actgtcgaca
    11161 atggattcgt attttttaag ggaatcgaag accgtgcctg gacctgcatt ccaattgtcc
    11221 acgccctggt atcccctggg caggccgggc ggatcgtagg ggtgaacccc aagacggagc
    11281 tgatgttccg cgcagaccgg aatgatgttt tggagaaaat aagtgatcct ttcccagtac
    11341 tcctcgtaac cgatgatccc ccggctgccg gcagggagtt cacgccagtt gtcctcaagt
    11401 ttaaacgaat gatagctcga gcctcccctg ccgtcgacat gcccgtttcg gcgaatgggg
    11461 atcatggtcc agtgcatggt aactacttgg acgccgacca gggaggcttt cctgatattt
    11521 tccgttactt cctgcaattt tttctcccgg tcccctccgg gtttggcata gatgaacccg
    11581 gagtccatgc gaatggcttc ccaaaccata tttccccggt cacaatcgtc tttccatttt
    11641 ttcatctggt ccaggtccca ggctccttcc atgatattgg tcaggtggcg aaccccatgt
    11701 ctctggtgat actggaaatt gcggggattg gtccagttct tttgtttatc attccaattc
    11761 cagccgggct cccccatcac cgcatggtaa tcacccccaa catgatggaa atgattgatc
    11821 ttactttctg cctttgccgg tttatccgtc agagcagtca atggacccaa tagtcccgga
    11881 cctatcagtc ccggacttat cagtcccgga cctagcagtc ctgcggcaat ggatttatgg
    11941 aaggaacgac ggttgagttt ttgtgtggtg gacttatctt tcatggaccg cttcatttga
    12001 ttgtttaaag caaaataggc agaaaatggc caagctacga atcgatattt tgcgcagggc
    12061 gtctcgaatt acacggaata ttttttagtt caaaaaaaat cctacctctc cgtgctcccc
    12121 gtgctctccg tgcgcaaaag cccgtgagca tattcaccga tcaggccggt ggccagatgt
    12181 ttatggaagg agcggcgggt ttagtttttg agtgttggcc ttttctttca tggacccgtt
    12241 tcaattgatt gcattgaaca aaatagggag aaaacagctc aagctaatat ggatactttg
    12301 ctcagagggt ataaccatcc cgcgccaagt cggagggtat cgaatgacac ggaatcttta
    12361 tgatattaaa aagtatccca aatctccgtg ctctccgcgc cctccgtgtg caaaaccccc
    12421 gtgtgcaaaa tcccgcgcac catcaccaca atcctttcaa tttatcatac atcgccctga
    12481 tcgaccggtg attcgacacc gtattccctc cccgtaccgg atgcttgctg aggtcaatct
    12541 ctttcacgag gtcctccttt gacttgcctg tttcaatccc tttacgcacc tgggcgagca
    12601 tatccgcgag gtaatccctc tgctgtttca ggatcccaga accaccaggg tctccatgcc
    12661 cgggaatgac cgtcgaggct ccccaggagg ccaggctatc caaaacccgg agccagcgat
    12721 cataatccgc gtttttatcc gctacattat tgccccatgg atttccgttt acacaaagat
    12781 cccccgtggc caggatcttt tctttgggca gccaggccac cgcgtccccc gcggtatgcg
    12841 ccggtcccat aaggatcaat tccacccggt gcgtaccatc atcaaaaacc attcgcctgt
    12901 caaaggtcag agaaggaaac tccctccggt agggacccat atccccaccg ctgtatgcgg
    12961 atcgccggtc ccattccact tgcccaaggg tcctcatctc cccgtcggtc accctggaag
    13021 caaccaccac cgccccggaa tctacaaaca cgctgttgcc ccaggtatgg tcgtgatggt
    13081 agtgggtgtt gaacacaaaa cggaccggtt tatccgtggt tttccggatc tcccggagga
    13141 tttcttccgc tccccagggg taattggcat ccacggccag gacataatct ttaaagacca
    13201 cccaaaggca attggcggac tttttctgaa gttcatcacc gaagtaatag aataccccgg
    13261 gagccagcgc ctgaacggga cgggattggg cgtttaaata gaaattgttc accaagaaca
    13321 ggaacaacag cacacccttt actttgaaaa aaggaatcgt tattctatcc gcttgttcca
    13381 tattcataga tatgccaaat tttcgaaatg gggcatatct caatttaaaa tcccgaaagc
    13441 cccgctctct gcaagaatgt aaagtcccaa aacaatcagg atcaatgggg cgaggatatg
    13501 cccatattta tcagcagctt tggatataat ggggtgcccc gccaggtatt tggcagccag
    13561 gcaccacagg aaggtcatga tgataaagac gcccaccgtc atcccctttc cctcccagcc
    13621 catcgcggcg aacaggggga cgtagacccc ggtattgtcg cccccgttgg cgatcgtgac
    13681 ccctgcaacg gcaaggacat tgcccatatt attttgcatt ggctctgcaa agtcgatttt
    13741 tacctcctgc tctttcttcc tgaacaggcg ccataagctt tttaaaccca aataaatagg
    13801 gaaaagacca aggagtccaa ggtatgccgg atccatccag agacccacga gggaaagggc
    13861 taggctcagt cccgtaaggg caagaatccc cagatactga ccaactatga tttccagggg
    13921 catgtacctt ttatttccaa accacatact gaggatgaac agatcgtcga tgttggtgga
    13981 tgcgaaggtg aggatgccgg tgaggatcag gtccatgggc ggcagattgg aaaatgttcg
    14041 acaaccttcc aggggcccca ttgctcctgt tcaatcaggc tgacggtgtc aaagttcaaa
    14101 acaatgggca atgcgacaac cctgatctgc cggaaaattt catccgtaca gcatgaattc
    14161 ctgggatgca tcagggtgat atggggtctg tgattgcggg ggatgtcaat gattccggtc
    14221 aatacggctt ttcgtaaatc atcaaacccc ggattcccta taaggccggg aaggagtacc
    14281 cccttaccat cctcaaaccg ctccacaggc ccgaattcaa tccggagcgg cccggggaga
    14341 atgatggatc ccaggttttt taagaccttt tcaaggggtt caatctcgtc ttcccggcat
    14401 aaggttatat gagcggggat aagattgaat tgttcgggat taaaagcagc ccggattttt
    14461 tcgatcattc cattctggtc agaaataaag agggtcaatt gccggcggct ctctcgcatt
    14521 ttacaacatt acaacaatcg attcggaatc agaaaggcta agggtagcca aaatatagaa
    14581 atccggctaa tctcagatgg tctattttac tttatatcag acctttttaa aaacgacctg
    14641 gctttttcca gctccggtcg ttcagatcgg tttggattgg ctaccgaagt cagcaggtca
    14701 tagaatgatt gtgctttttc cgggttattg gattttgctg cggccaggcc agccccataa
    14761 aggctgttga accggtttgg atgccttttt aaattgattt cataagcctc aagcgctttg
    14821 ccgggttgat ttacttgaag caacatgtcc cctagtaatt cccttgccgg gatcacctcc
    14881 ccgggcgtga ccgggtgttt ttcagtatta tcctccattt gggcggccat atccatcagt
    14941 ctcagggctt cggagttttt gccttcttgc agctgtatcc aggctttggc gctgtttatc
    15001 tggatcagta cctggttggc attatatgtt tcttttttag ccatgagggc atcgtaaatt
    15061 ttttctaaag cagccagttc caaccgggca gaatccaggt ggccaaggtg gacattacca
    15121 aggagccgcg taaaatggac gatcgcggtt tgccaggggt aatcctgcca taaaaaattg
    15181 atcttcggga gctgcaaggt ggcggcttcg ttccagagcc tgttttcaag cacaaagcga
    15241 gaaggaatgg ctgcgaaggc ataagcaacc ttaaagttcg ttgggctcac ctcgtggatg
    15301 gtgttcaggt attccaattg ctgccgggcc aggtcatttt cccctttttg caggtaggca
    15361 tacacgaggt agtccatggc atgaagttcc tcatcccaat ggccttttaa tccggcgttc
    15421 tctgcataac attttgcaga agctgcagac acgagattgg attggataca ttcgtcccaa
    15481 agccccagcc tcgtgaaaat atgcgacggc atgtgcaagg catgggcgga ggagggtgct
    15541 atggacatat atttcctcgc cgcaggcagg gccagttcgg ccagctcagg ataatcgcaa
    15601 ctgtgaatga tataatgcac aatgccggga tgatccggtt cattgggata aagggaggtt
    15661 aaaatggagt gggctttctt ttgcctggta aaggttttgt ctgcaggatc cgccgcggcc
    15721 acgagggcca gggaataata aatggccgcc tctatatcgt cgggccaggt gtaatagagt
    15781 ttttccattg ccttctcaaa acgcatactc ctggtacgat gatccgtatt ttgccaatct
    15841 tcataaaaca aggaaagcgc ttcgaggtaa tccgattccc gcctgggaat tcgctccaag
    15901 ccccttgcaa cggcaagagc cctggagcct ttttccaact ccgcttcgga gggcggggcc
    15961 cagaggggat ggtaattgct catggccact ccccaatagg ccatggcgca atccggggat
    16021 tgatcaatta ttttggcaaa gaccttttct gcttcatcgt attcaaagga atgcaataaa
    16081 gccatagcca ggttaaaatc tttacctcca ccaacggaac aggaggtttc aaactcaacg
    16141 gtaccaaacc ggccatccgg cgggccgcac cacgtgacct cccctctctt taaattgatt
    16201 aaaccgatgg actcaagcga tggaatttta ttcttctctt tgcaggaaaa gaacaggata
    16261 aaacttaaac aaaaccaaaa aggatggatt tgcacgcagc gtttcatcca tttctaacga
    16321 atagcctggt ctgagaattg ttttaatctc taattgagca caaaccatcc ttaggtcagt
    16381 ttttgaaatt ttgcctttcc ggtaacctta ccgttttttg actcgccggg atattacctt
    16441 cactcctgta ttttatttta gttttaactg cttttttaat aaccaggatt ctttgtatgc
    16501 taagaataaa tcaggaactc accttaaacg acctcaaggc caaaatatcc gctttctggg
    16561 acctttccgg tggaaaaatc agggcccttg aaaaggaata cgacccctcc aaagggtctc
    16621 ccgtgttcac cgcggccgga aaatatacca cccggggatg gaccgagtgg acccaaggct
    16681 tccagtttgg gtcggcactt ctccaatacg atgccacggg agaagattgg tttcacaaca
    16741 tcggcaggga ccgtacgctc ggattcatgg ccccgcatat cagccatgta ggcgtgcatg
    16801 atcacggatt caacaatgta agcacctacg gcaacctcct ccgcctgatg caggaaggca
    16861 aaacgccgta caatgaatgg gaaaaaaatt tttatgaact ggcccttaaa atatccgggg
    16921 cggtccaggc cagccggtgg actaagatca aaaacggcgg attcatacac tctttcaacg
    16981 ggccccattc cctttttgtg gatacccttc gttcctgccg ggccctgatc ctcagccacc
    17041 tgttggggca tgaactccag gctgaaaacg atatccggat cagtttactg gaccgggctt
    17101 tccagcatat ccgggcaacg gcggactatt ccgtgtttta tggccaggga cgtgacagtt
    17161 acgacacctg gggtcgcacc gcccatgaat gtatttttaa tacgcgggac gggaattacc
    17221 gctgtcccaa ctcacaacag gggttttcgg gattcactac ctggacccgc ggactggcct
    17281 gggcgatgtg cggtttctcc gaacagcttg aattgctcaa agaattatcc tccgatgaaa
    17341 ttttgattgc aggggagaga gaactgattg aagccatgat cctgaaagcc gcccgcgcca
    17401 cctgtgattt ttatatccag aataccacga ccgacggtat accctactgg gacaccggag
    17461 cgccggggct tgtaaaaatg ggtgattatc tctcccggcc ggccgagccg tataacgatc
    17521 atgaaccggt ggacagttct gctgccgcta ttggatgtca ggggctcctg cgttttggcc
    17581 tctacctcac cggtaaaggc gatgaggagc tgggtaccca ttattggcag gccggcctga
    17641 ccgtattgaa taccctgctg gaccagcctt atctgagctt agatgccgga caccagggct
    17701 tgctcctgca ttccatctat caccgtccga acggttggga ctatgtcccg gcgggaagta
    17761 aaataccttg cggagaatcg agcatgtggg gcgattacca tatccgcgaa gtcgccttat
    17821 acgttcaaaa acttctccgc caggaacctt attacgcctt tttcaaggcc gttaaaaaag
    17881 actgagatga gcaggctgac ggattattca aggctttgca tccataccat cacgactaaa
    17941 ccctggagta ttgaagaagc ctgtggaaat ttttcagatg ccggcgtcaa aggcatcacg
    18001 gtatggagaa atgcactgga gggaaaggcc ctcatggcta ccggccggat gatccgggac
    18061 cgtgggctgc agatcgtctc cctttgccgg ggaggttttt tcccatccat tgatgcggta
    18121 aagagaagcc tggccctcga ggataaccga aaggcgattg atgaagccca tgccctcgga
    18181 gcccctatga tcgtgctggt ctgcggagcc gatccgggac aacccctgca agagtcccgg
    18241 aaacaaatcc gggatggaat ac
//
LOCUS       DAOQXT010000098        18035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3504_length_18035_cov_6.789989, whole genome shotgun sequence.
ACCESSION   DAOQXT010000098 DAOQXT010000000
VERSION     DAOQXT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18035
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3504_length_18035_cov_6.789989"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="PKM27_14990"
     CDS             <1..1553
                     /locus_tag="PKM27_14990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNR08624.1"
                     /translation="TVGGLSRNKAGYGFIYDGLDRMTGSRYGENTGSWTNLNRYNENL
                     SYDLNGNILTLGRRGYISAGNWNTIDTLTYTYGNSSNPDWATTIADGSGSALGYPNTS
                     GTFSFDAAGNQTTYSAKGITAITYNHLNLPTKYTWSGGNTLEIQYDAAGRKLKKIPST
                     GNAKTYVGGIEYSASAIEAIYHPEGRARLISSTWEYEYAVKDHLGNARVYFKYNGSIS
                     LLQESHYYPFGMEQGGWTAPTSPENKYKYNGKELNGEFSLGMYDYGARWYDPTVGRWG
                     QVDPMAEKMKEWSLYNYAFNNSISFNDLDGNEPNDPVEPPNWIELLKRGFKDFNEAIK
                     GLLKSIGFDSETVLVGPKSDEEASAITRNRAIMDNFKDDLQQIVDIQTSLIPGGSLIN
                     PNSTKSDIATDIGLETAGLILPGLKFIGTVRINYLNNVQNPKLRNLINDLYRPFAKVG
                     SGSSMDAYRLEQTTGRNVGGKSHGTKLIEYRTALIKLWNENIALSPSDKAIVKQLLTD
                     IQNALSGN"
     gene            1534..1893
                     /locus_tag="PKM27_14995"
     CDS             1534..1893
                     /locus_tag="PKM27_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08625.1"
                     /translation="MHFQEIKDEYYLKINKRLRETNPQFKSVFDIDDGVYLILGEFGT
                     YLLDHINEDDKFSDALCFINEALEYGKHETEDAIVIQIFHKIYASSIDISKFRDYLNP
                     RAKFVFDTQLTKFNEPI"
     gene            2490..3515
                     /locus_tag="PKM27_15000"
     CDS             2490..3515
                     /locus_tag="PKM27_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNR08626.1"
                     /translation="MKRRRSNIVNLARNGYISSGILNAFNNLTYTYGNSSNPDWPTTI
                     ADGSGSALGYPNTSGTFSFDAAGNQTTYSAKSITAITYNHLNLPTKYTWSGGNTLEIQ
                     YDASGRKLKKIPSTGNAKTYIGGIEYSASVIEAIYHPEGRARLISSTWEYKYAIKDHL
                     GNARVYFKCNGSITFLEESHYYPFGLEQGGWTAPTSPENKYKYNGKEMNDEFSLGLYD
                     YGARWYDPTVGRWGQVDPSSENDRSISPYLYAFNNPLHFTDPDGKWPYPGVMPLFKSI
                     SNFFKGSDAGLNQQYSLYKMNGRTSGGDTKLGIQDNALGRLKNGKNVGAPVVRFDAAD
                     TKTPFHM"
     gene            3536..3940
                     /locus_tag="PKM27_15005"
     CDS             3536..3940
                     /locus_tag="PKM27_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08627.1"
                     /translation="MHLPLAGGEKTLTALAGAGNTLDAISKVAKPVAIATDVIRLGDA
                     VTSDGGRIGDNTIVAAGSVAGGWAGAWGGAVLGAKGGDIAGLLGGPFAEVTVPIGGFV
                     GGLVGGIVGAFGGSKLGEEGSKQLIKIQEEIK"
     gene            3937..4425
                     /locus_tag="PKM27_15010"
     CDS             3937..4425
                     /locus_tag="PKM27_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08628.1"
                     /translation="MILINQYQILEVYFPRESLNKETWSFWTKTISTLLFQLSKGPMS
                     IFTVNPDKSRSIGYELGIATEGKLHSKKYLNSILNPVIQQDLDLLVDTDEFYLGHTIL
                     VNSDLNESKIENIIICWSHNLFKSEVEFFKMDSDGLCFYWYNPKNIEEAKRYLTGLQW
                     PI"
     gene            4482..4700
                     /locus_tag="PKM27_15015"
     CDS             4482..4700
                     /locus_tag="PKM27_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08629.1"
                     /translation="MTYESQGSILALTKKEYISAGNWNTIDTLTYTYGNSSNPNWATT
                     IADGSGSALGYPNTSGTFSFDAAGKLIH"
     gene            complement(5380..6375)
                     /locus_tag="PKM27_15020"
     CDS             complement(5380..6375)
                     /locus_tag="PKM27_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HNR08630.1"
                     /translation="MFTIQSYPLAVLFCILTMICWGSWANTQKLAASTWRFELFYWDY
                     VAGILLFSLLFAFTLGSLGDSGRSFVEDLYQASGSSLFSAFIGGVLFNAANILLVAAI
                     AIAGMSVAFPVGIGLALVVGVIVNYLAAPVGNSVLLFTGVVLVAVAILLNANAYRKLA
                     SNTGKVSTKGLALSVVAGLLMGFFYKFVAGSMFPDFAHPLDGKLSPYTAVVVFSTGIL
                     ASNFLFNTLLMRRPFVGTPVSFSDYFSGNFRTHAVGILGGMIWCAGMSFSILASDKAG
                     PAISYGLGQGATIVAALWGIYIWKEFKGAPAGVHRILNLMLACYIAGLAIIIYSR"
     gene            complement(6447..7334)
                     /gene="rbsK"
                     /locus_tag="PKM27_15025"
     CDS             complement(6447..7334)
                     /gene="rbsK"
                     /locus_tag="PKM27_15025"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNR08631.1"
                     /translation="MLVIGSSNTDMVVKTSRFPAPGETIIGGDFFIFPGGKGANQAVA
                     AARLGVRTTFICRTGDDLFGRQALEGFRNEGIDTTYCSVDPEAASGVALITINAEGQN
                     EIVVAPGANDRLMPEHLDAAKSAFTNNEYVLFQLEIPMATVSHGIQMCRDLGIKAILN
                     PAPAALLDKNLYPKLYLITPNEKEAEMLTGISIDDLASADKAADRFLAWGVKQVIITL
                     GAGGAYFKDGEQAFHIPAPIIQAVDTTAAGDIFNGALAVALSQGQSWKEAVRFAVKAA
                     SLSVSRLGAQASAPYLYEI"
     gene            complement(7447..8763)
                     /locus_tag="PKM27_15030"
     CDS             complement(7447..8763)
                     /locus_tag="PKM27_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08632.1"
                     /translation="MNRRKFISQSSQWSLAAGWTAIHPGLAPLLPRKSANEKILVGLI
                     GANSMGWGILSHALRQPNVECIGICDVDQNVLGRRTEEVAKQYGKKPQAFSDFRKLLE
                     LKDLDAVIIGTPDHWHCLIMVEACWAGKDVYVEKPMANSIAECTVMVRAARKYKRIVQ
                     VGQQQRSGKHWQEAMDFIHTGQIGSLRKVHAWANFNYGTGQPKAPDEPIPAGVDFDTW
                     LGPAPSRSFNKTRFHGSWRMFWDYGGGLMTDWGAHLIDIALWAKKITSPSGYVMAGGG
                     NFYSPDFAHETFDTQSVLYQFPDYTITWEHTAGIQSGPYNRPYGLAFLGNDATLVIDR
                     GGWEIFPESEGGKFKTPAFPKRSGRESHEEHVRNWLECIRERKEPNCTVENGRLTAMY
                     CHFGNIALRTHSLLDWNETSHSFGQNQSANRLIKPEYRKPWVFPEE"
     gene            8873..10171
                     /locus_tag="PKM27_15035"
     CDS             8873..10171
                     /locus_tag="PKM27_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR08633.1"
                     /translation="MGKIRHYRWIVVGLLFAATAINYIDRQVIGLLKPLLEKEFNWTE
                     SQFARIVMAFTLAYAIGLLLSGRVIDRIGTKLGFAITITVWSIAGMLHALARSVWSFS
                     LVRIILGLGEAGNFPAAVKTVAEWFPKNERGLATGIFNSGTSVGVVLSVLLVPLILSF
                     GSWQQVFWITGALGFIWLAAWWVFYEVPSKQKRLTAEEYEWIRSGQEEAAQVQVAIRW
                     KKLFAFRQTWAMITGKGLIDPIFWFFLFWLPSYFSSTFSLDLKKPSLELMIIYTATTL
                     GSIGGGYLSSLLIKKGWPVLKARKTVLLIFAFTELSVFFTQFANDAWVAVLLISLAVA
                     VHQAWATNIFTLATDLFPKEAVSSVAGIAGMAGAVGGIFFPLLVGYILDRFKAVDHLT
                     GGYHLIFSICAFTYLLAWLIIHLLTRKSGRVELAELIPKG"
     gene            10354..10623
                     /locus_tag="PKM27_15040"
     CDS             10354..10623
                     /locus_tag="PKM27_15040"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNR08634.1"
                     /translation="MTRFDTRLTKEQKLLFKRAAMLGGYRNLSDFVITTVQNRAVEII
                     ETKERIIASQRDLDIFFEAITRPAKPNKALRSAARGFKSGIRSGH"
     gene            10613..11116
                     /locus_tag="PKM27_15045"
     CDS             10613..11116
                     /locus_tag="PKM27_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08635.1"
                     /translation="MDTETVLLGSRHRKTDFLCGKVQLDHYLHHQAGQDVKRKLAACF
                     VSVDPVNERIRGYYTLSNYSLPLAGWPEQIKKKLPKSYLAIPVTLLGRLAVDHRYQGQ
                     GLGEWLLLDALYRSYTSSEIIGSLGVVTDPLDIEAENFYTKYGFIKLPDSGKMFIAMQ
                     TIIQLFQ"
     gene            complement(11402..11572)
                     /locus_tag="PKM27_15050"
                     /pseudo
     CDS             complement(11402..11572)
                     /locus_tag="PKM27_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929977.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
     gene            12254..13615
                     /locus_tag="PKM27_15055"
     CDS             12254..13615
                     /locus_tag="PKM27_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNR08636.1"
                     /translation="MKRRNLLKNLAAASAAATLPLQQVLAKNTIPKMQASGDIRITDI
                     KTILTAPDGIRLVVVKVMTSEPGLYGIGCATFTQRAYVVATAIEKYLKPFLTGRNVDE
                     IEDIWQSSYVSSYWRNGPVLFNAMSGVDIALWDIKAKRANMPLYQLLGGKVRHGVDLY
                     YHASGLEYSELDDKVAAAIANGYRHVRIQMGQKGNANYGARGATGTPASSVGLPPEQS
                     GPTDPKIIFNPAVYTRSVPKMFEHIRVKFGEEVELLHDIHERIPLNAAVNMCKELEQY
                     HPYFIEDPFPPEDNDHFKILRQQTTVPLAMGELFNTQQEFLPLIKDRLIDFIRVHISQ
                     IGGLSPARKVQALSEFFGVRTAWHGPGDASPVAHAAQLALELCSYNFGVHEGYVFPEK
                     TKEVFPGCPETRNGYMYAQEKPGLGMDIDEKAAAKYPFAEGKNFDYSWGTTRRPDGTV
                     IRP"
     gene            13627..14379
                     /locus_tag="PKM27_15060"
     CDS             13627..14379
                     /locus_tag="PKM27_15060"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR08637.1"
                     /translation="MGLRLPLFAARDLPFTLIKLWLLVYLVLGIRGRICGQQDPEAGY
                     KLIFNGKDLEGWKGDPVYWRVEDGILTGEVSPATLLKKNSFIIWQGGKPGDFELKLEY
                     RVSSKGNSGINYRSVLVDGEPYALKGYQADIDGEDQWSGQNYEERGRTFLARRGQQVR
                     VDPGPRISLTAELGKPGDLQQYIRKEDWNEYHLIVKGNHLRHFINGKLMSEVLDLDTI
                     NRTSEGYLGVQVHVGPPMKIEFRNIRLKPLNP"
     gene            14429..14782
                     /locus_tag="PKM27_15065"
     CDS             14429..14782
                     /locus_tag="PKM27_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08638.1"
                     /translation="MFQTNSLWVEIAVVSIFYLLGHIFMGHFAERDPKWRKFAKYLVT
                     LVLVMLLSTYFGRSVSMTVLGLSLLPILYIHAVILPRKGINGWTGEPKSRYYDFRGWN
                     KDIFGTGRTPGNPEG"
     gene            complement(14954..16105)
                     /locus_tag="PKM27_15070"
     CDS             complement(14954..16105)
                     /locus_tag="PKM27_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08639.1"
                     /translation="MTKKIKVAVAGVGFIGPVHIESLRRVGTLDVVAMFHPIENEAKD
                     KAAALGVPAYYTDYDQMIREADFDCIHICTPNNLHFSMAKAALLAGKHVVCEKPLATT
                     VKEAEELIALAKEKKAVNAVSFNIRYYPLVHQMKAMREKGEIGRIYSIIGSYLQDWLF
                     FETDYNWRLEPDKSGESKAIADIGSHLIDLIEYISGLSIVEVMADFSTIHPIRKKPLK
                     PVETYSGKLLTPEDYSDVPIATEDYASVLLRFNDGCKGVVTVSQVSAGRKNRINLEIA
                     GSKQTLAWCSETPNELWIGNRNEANAVMLRDPSLVDKSVRSIISYPGGHNEGFGSTSK
                     QLFTEIYKAVAEGKQPEHPSFPTFEEGWRELLLCDRIIESHRTQKWVKV"
     gene            complement(16235..17614)
                     /locus_tag="PKM27_15075"
     CDS             complement(16235..17614)
                     /locus_tag="PKM27_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HNR08640.1"
                     /translation="MKTAKGLLICFLLFQSMTALAQLRVSDNKRFLQTADGKPFFWMG
                     DTAWELFHRLSREEADKYLKTRADQGFNVIQAVVLAELDGLHTPNAYGEIPLDHDDPT
                     RPREAYFQHVDYIIEKAASLGIYIGLLPTWGDKLNKSTWGTGPEIFNAENAKQYGLWI
                     GRRYKNKKNIIWILGGDRTPRHDADVNIWRAMAAGITEGAGGPDRALMTFHPQPNAVQ
                     DGGSAKWFHQDDWLDFNMLQTGHCRENNVWDRIQYPYNLKPVKPVLDGEPIYEDHPVC
                     FNARENGTSSAYDVRRSAWYTVLAGAFGHTYGCHDIWQMYAPHRTPVNGPHHPWYVAL
                     DLPGANQMKYLRYLFESRPMFDRIPDQTLLSTHGGNNDRLQASRGKDYIIVYSAQGNT
                     FTLYTGKLSATQLTAHWYNPKNGQSQALGTFENKISQEFIPPSSGYGHDWVLVVDDAV
                     KNYTMPAFN"
BASE COUNT         4661 a         4202 c         4452 g         4720 t
ORIGIN      
        1 gtaccgttgg gggcctgtcc cggaacaagg ccgggtacgg ctttatctat gacggcctgg
       61 accggatgac cggctcgagg tacggtgaaa ataccggctc ctggaccaac ctgaaccgct
      121 acaatgaaaa cctgagctat gatcttaacg gaaatatcct gaccctgggc aggagaggat
      181 atatcagcgc cggcaattgg aataccattg acacgctgac ctatacctat ggcaattcct
      241 ccaatcctga ttgggccacc accatagccg acgggtccgg gagcgccctg ggatatccca
      301 atacctcagg cacctttagc tttgatgccg ccggtaatca aactacttat tctgccaaag
      361 gcatcacggc cataacttac aatcacctca atctcccaac caaatacacc tggagcgggg
      421 ggaacacttt ggagatacaa tacgatgcag ctggcaggaa acttaaaaaa atacccagta
      481 ccggaaacgc taaaacttat gtgggaggta ttgaatacag cgcctctgct attgaagcta
      541 tctatcaccc ggagggaagg gccaggttga tcagcagtac ctgggagtat gaatatgctg
      601 ttaaggatca cctgggaaat gcacgggtat actttaaata caatggaagt atcagtctct
      661 tacaagagtc tcattactat ccatttggta tggagcaggg aggatggaca gcaccgacca
      721 gtccggagaa taagtataaa tataatggga aggaattgaa tggtgaattt agcttgggga
      781 tgtatgatta tggggcgagg tggtatgatc cgacagtagg gagatggggg caggtggatc
      841 cgatggcgga gaaaatgaag gaatggagtt tatacaatta tgcttttaat aattcaatta
      901 gctttaatga cctagatggg aatgaaccca atgatccagt tgaaccccca aattggatag
      961 agctgcttaa aagagggttt aaagatttta atgaggcgat taaaggtctg ctaaaatcaa
     1021 taggattcga tagtgaaaca gtcttagtcg gtccaaagtc agatgaagaa gcatccgcca
     1081 tcacaaggaa tagagcgata atggataatt ttaaggatga tcttcagcaa attgttgata
     1141 ttcaaacttc attgattcct ggagggtcgc taattaatcc caattcaaca aagagcgata
     1201 ttgccacaga tattggatta gaaacggctg gtcttatttt gccaggacta aaatttatcg
     1261 gtactgtaag aattaattac ttgaataatg ttcaaaaccc taaactaagg aatttaatta
     1321 atgatctata tcgacctttt gccaaagttg gtagtggaag ttcaatggat gcttatagat
     1381 tagaacaaac gactggaaga aatgtcggag gtaaatcaca tggtactaaa ttaatcgagt
     1441 atcggacggc attaataaaa ttatggaatg agaacattgc tctttcccca tctgataaag
     1501 caattgtaaa gcaattatta actgatatac aaaatgcact ttcaggaaat taaagacgaa
     1561 tattatttaa aaattaataa aagattaaga gagactaatc ctcaatttaa atcggttttc
     1621 gatattgatg atggtgtgta tcttatactt ggagaatttg gaacttactt attagatcat
     1681 attaatgagg atgataaatt ttcggatgct ctgtgtttta ttaacgaagc tttagaatat
     1741 ggcaagcatg aaacagagga tgcaattgta attcaaatat ttcataagat ctatgcatct
     1801 tcgattgata ttagcaagtt tcgagattat ttaaacccca gagcaaaatt tgtttttgat
     1861 acccaactaa ctaaatttaa tgaaccgatt taaattaaat taatattaat agccgtacaa
     1921 ggtaaacctt ctaaggatgc ccgtcaatta gcctgcagct ccccctaaag tttcagatgt
     1981 atttactaat aaattttaat gagtacttat atattattac tcttcatttt tactttaaac
     2041 agcaaaatga atgcctgtga tccgtgtatt ttgataaata aatcttttca aaatgaatta
     2101 ttccagaagg aattttatcc atgtaatgat tcatctgaca ctttaattct tattgataat
     2161 agtaaggtga ttcctgtaaa ttgtaaaata gatcaagtat gctcaagacc tattaagctc
     2221 agctttactg ataaaagtga tctttcgcca gaaattatta tattatatcg atatgacaac
     2281 aaccgtagaa aaaaggctat ttatttaatg cggccattta caggagctgc acttatattc
     2341 agatttgaaa ggagaaagag agtttgttca ttaattaatg tcgaagttgg ctctttttaa
     2401 tttaataatg caggataatg aactgatttg tttcggtata atttaaatct cctatatttt
     2461 agtaatgctt gccatcccga gatgaaacaa tgaaacgaag acggagtaat atagtgaacc
     2521 ttgcgcgcaa tggatatata agctcaggaa ttttgaatgc gttcaataat ctgacctata
     2581 cctacggcaa ttcgtccaat cctgattggc ccactaccat agccgacggc tccggaagcg
     2641 ccttaggcta tcccaatacc tcaggtactt tcagcttcga cgctgccggc aatcaaacga
     2701 cttattctgc aaaaagtatt actgccataa cctacaatca cctcaacctg ccaactaaat
     2761 atacctggag cggggggaac actttggaga ttcaatacga cgcttctggt agaaagctca
     2821 aaaaaatacc cagtaccggt aatgccaaaa cctatatagg aggtattgaa tacagcgcct
     2881 ctgttattga agctatctat catccggaag gtcgggccag actgatcagc tccacttggg
     2941 agtataagta tgctattaaa gatcatctgg gaaatgcacg ggtatacttt aaatgcaatg
     3001 gaagtatcac attcttagag gagagccatt attatccttt cggcttagag caaggaggat
     3061 ggacagcgcc gaccagtccg gagaataagt ataagtacaa tgggaaggaa atgaatgatg
     3121 aatttagctt gggattatat gattatgggg cgaggtggta tgatccgaca gtagggagat
     3181 gggggcaggt ggatccgagc tctgaaaatg accgcagcat ttcaccatat ctttacgcgt
     3241 ttaataaccc tttacatttt acggatccag atggcaaatg gccatatcct ggagtaatgc
     3301 cactatttaa atctatttct aattttttca aaggttcgga tgcaggctta aatcaacaat
     3361 attctttata taagatgaat ggaagaacat caggtggtga tacaaaactg ggtattcagg
     3421 ataatgcgtt aggacgttta aaaaatggca agaatgtcgg agcgcctgta gttagatttg
     3481 atgctgctga taccaaaact ccattccaca tgtaaatggt cccaaaggtt caaatttgca
     3541 cctaccttta gctggtggag agaaaacttt gacggcactt gctggtgcag gcaatacatt
     3601 ggatgctatt agtaaggtgg ctaaaccggt tgctatagcc acagatgtca taaggttagg
     3661 tgatgcagta acatctgatg gcggaagaat tggtgataat acaattgtcg ctgctggaag
     3721 tgtagctgga ggttgggctg gtgcgtgggg cggtgctgtt ttgggagcaa agggcggaga
     3781 tatagctggt ctattgggtg ggcccttcgc cgaagtaaca gtcccaatag gagggtttgt
     3841 tggtggattg gttggtggga tagtcggcgc ctttggtggt tcaaaattag gagaagaagg
     3901 ttcgaaacaa ctaataaaaa tacaggagga aataaaatga ttctaattaa tcaatatcaa
     3961 atacttgaag tttactttcc tcgcgaaagc ttaaataaag aaacatggtc tttttggact
     4021 aagacaatat caactttatt gttccagctc tcgaaaggac caatgagtat ttttactgtc
     4081 aatcccgata agtctagaag cattggatac gaattaggga tagctacaga agggaaatta
     4141 cattctaaga aatacctaaa tagcattctt aatccagtaa tacaacaaga tttggatttg
     4201 cttgttgata ctgatgaatt ttatctggga catacgattc tcgtcaattc tgacctcaat
     4261 gaaagtaaaa tagaaaatat tataatctgt tggagtcaca acctttttaa aagcgaagtt
     4321 gaatttttta aaatggactc agatggcctt tgcttttact ggtataatcc aaaaaacatt
     4381 gaggaagcta aaagatactt aactggttta caatggccta tctaacttat taagaataaa
     4441 acaactttaa ttgacaatat tgggaaattg aaatgatagg gatgacctat gaatcccaag
     4501 gcagtatcct tgccctgacg aaaaaggaat atatcagcgc cggcaactgg aataccattg
     4561 acacgctgac ttatacctac ggcaattcgt ccaatcccaa ttgggccact accatagccg
     4621 acggctccgg aagcgccctg ggatatccca atacctcagg caccttcagt tttgatgccg
     4681 cgggtaaact gatacactga taccggctaa gatcataagg gaggaacgga ggagtgtgac
     4741 ctaaaattta atctttcttt tttttagctc ctcaatgatt tttgggctga gcccgcggca
     4801 acggcaatgc ttgatatgct ccaaatgcca ggcaatgcgc tggtccatgg tcggattttt
     4861 tggcatttta tttttcaaat gccattcggc gttgagtatt ggtagttcgg gcataggatt
     4921 tgttcctttt gaaaggcaat atactcaatt gagacaaccg acagtcataa gttgagccca
     4981 ggaatatctt aaaaaattga taatcctttt ccattatgat tgttgagatc ctggttttgg
     5041 ctaaagccca tttgcctttt cttgatcaaa ccctcaggct gaagcctggg gttagcagtt
     5101 aaattggttg ctggcggatg taatcctcgg gcttaatatt taggataagc gcctaagtcc
     5161 ttgcccttgt atttccctgc agacctagtg tcaatctagc agagtagtta cttgatcagg
     5221 ttaaatctct gactttgggc taatgcctcc ttgccatctc aggagccaac cgccagcctg
     5281 aggcctggga ttgtctgaat taactggagg caggcttgtc atgggcttgc ggaacccagg
     5341 cttttttgct tcttcctatt caggggccca gaagatcgct taccttgaat aaatgatgat
     5401 cgccaggccg gcaatataac aggccagcat gaggttcaag atccggtgga ccccggccgg
     5461 tgcaccttta aattccttcc agatatagat cccccacaga gcagctacga tggtggcccc
     5521 ctgtccaagt ccataagaga tggccggtcc cgccttgtcc gaagccagga tgctgaagga
     5581 catgcccgcg caccagatca tgcctcccag tatgcctacc gcatgggtcc ggaagttgcc
     5641 cgaaaaataa tctgaaaagg aaaccggtgt acccacaaaa ggccgccgca taagcagggt
     5701 attgaataaa aaattgctgg ccaggatgcc ggtggaaaag accaccacgg ccgtataggg
     5761 actgagtttg ccgtccagcg ggtgagcaaa atcggggaac atggagccgg ccacgaattt
     5821 atagaaaaaa cccatcagaa gaccggcgac tactgaaagc gccaagcctt tggtggacac
     5881 tttcccggta ttggaagcaa gtttccggta agcgttcgca ttcaggagaa tggcaacggc
     5941 taccaggacc accccggtaa acagcaatac ggaatttccc accggggctg ccaggtaatt
     6001 gacaatcacc ccgaccacca gggccaggcc aatgcccacc gggaaagcaa ccgacatgcc
     6061 cgcaatcgca atcgcggcga ccagcaggat attggccgca ttgaaaagaa ccccgccaat
     6121 aaaggcggaa aagagtgaag agccggaagc ctggtacagg tcttccacaa aactccgtcc
     6181 cgaatctcca aggctgccca gggtaaatgc aaaaagcagg gaaaacaaaa ggatgccagc
     6241 gacataatcc caataaaaca attcaaatcg ccaggttgag gcggccagtt tttgggtatt
     6301 ggcccaggag ccccagcaaa tcatggtgag gatgcaaaag aggacggcga gagggtagga
     6361 ttggatggta aacattatgt agtagtcagt ggtcagttag caagttagca agttagcaag
     6421 tttactgata gctgatagct gatagctcaa atctcataaa ggtagggtgc ggatgcctgt
     6481 gctcccaggc gggagacgga aaggctggca gcctttactg caaaacggac cgcttctttc
     6541 caggattggc cctggctgag ggctacggca agggctccat tgaaaatatc gccggccgcg
     6601 gtggtatcta ccgcttgaat tataggcgcc ggaatatgga aggcctgttc accatctttg
     6661 aaataagctc ctccggcacc cagggtgatg atcacttgct ttacccccca ggccaggaaa
     6721 cggtcggccg ctttatccgc ggaagcgaga tcatcgatgg agatcccggt gagcatttct
     6781 gcctcttttt cgttgggggt gatgaggtaa agttttggat aaaggttttt atcaaggaga
     6841 gcagctgggg cgggattcag gattgctttg atccccaggt cccggcacat ttgaattcca
     6901 tggctgacgg tagccatagg gatctccagc tgaaataaaa catattcatt attggtgaat
     6961 gcggactttg cagcatccag gtgctcaggc atcaaacggt cattggcccc gggagcgacc
     7021 acgatttcat tctggccttc ggcattgatg gtgatcaggg caaccccgga ggctgcttcc
     7081 gggtccaccg agcagtaagt ggtatcgatt ccttcgttcc tgaatccctc caatgcctgc
     7141 cttccaaaca aatcatcccc ggtccggcat ataaaagttg tgcgcactcc cagacgggcc
     7201 gccgccacgg cttggttggc tcccttgcct cccggaaaaa taaaaaaatc acccccgatg
     7261 atggtctccc caggcgcggg aaaacggctg gttttgacta ccatgtcggt gtttgaactt
     7321 ccgatgacca gcattgttga attgttgaag tgctaaactg ttgaattgtt gcggaattgt
     7381 tgaattgtgt tgttgtagcg ttgtactttt tctcccttaa cctttttcct ttaaccctta
     7441 accttttcat tcctccggaa acacccacgg tttcctatac tctggcttaa tgagtctatt
     7501 ggctgactgg ttttggccaa aggaatggct tgtttcattc caatcaagca gggaatgggt
     7561 acgtaaagcg atattgccga aatggcaata catggcggtt agccggccgt tttccacggt
     7621 gcaattgggt tccttgcgtt cgcggatgca ttcgagccaa ttgcgcacat gttcttcatg
     7681 cgattccctg ccgctgcgtt tggggaaagc cggggtcttg aatttaccgc cttccgattc
     7741 cgggaagatt tcccagcctc cgcggtcaat gaccagggtg gcgtcgttgc ccaggaaggc
     7801 gagaccgtag ggacggttgt agggtccgct ctggatgccg gccgtatgtt cccaggtgat
     7861 ggtataatcg ggaaactggt aaaggacgga ttgggtatca aaggtttcat gggcaaaatc
     7921 cggggaataa aaattgcctc cgccggccat gacataaccg gatgggctgg tgattttttt
     7981 ggcccaaagg gcgatatcga tgaggtgggc tccccaatcc gtcatcaggc cgcccccgta
     8041 atcccagaac atccgccagc tgccgtggaa gcgtgtcttg ttgaaactgc gggatggcgc
     8101 aggacccagc caggtatcga agtccactcc cgcgggtatg ggttcatcgg gcgctttggg
     8161 ctggccggtt ccatagttga aattggccca ggcatgtact ttgcgcaagc ttccgatttg
     8221 cccggtatgg ataaaatcca tggcttcctg ccagtgtttg ccgcttcgtt gctgctggcc
     8281 tacctgtacg atccgcttgt atttgcgggc ggcccggacc atcacggtac actcggcgat
     8341 ggagttggcc atgggtttct ccacatatac atcctttcct gcccagcagg cttccaccat
     8401 gatgaggcaa tgccagtgat cgggagtgcc gatgatcacg gcatccagat ccttgagctc
     8461 gagcagtttc ctgaagtctg aaaaagcctg gggttttttg ccatattgtt tggcgacttc
     8521 ttccgtcctc cggccgagca cattctggtc cacatcgcag atcccgatgc attcgacatt
     8581 gggctggcgg agggcgtggc tgaggatgcc ccatcccatg ctgttggctc cgatgaggcc
     8641 gacgaggatt ttttcattcg cggatttgcg gggcaaaagg ggagcgaggc ccggatggat
     8701 ggcggtccat cccgctgcca gggaccattg ggaactttgg ctgataaatt tgcggcgatt
     8761 cataagtaat aatttgaaac gggtcttttc aaagataaat tttttaattt gacagcggat
     8821 agcattggaa ttaagaatta agaattaagg aattaagaat taagaattgg gggtggggaa
     8881 aattcggcat taccggtgga tcgtggtcgg gctgttgttt gcggcgacgg cgatcaatta
     8941 tatcgaccgc caggtcatcg gcttgctaaa acccttgttg gaaaaagaat tcaactggac
     9001 ggagagccag tttgcgcgga tcgtcatggc atttaccctg gcttatgcga tcggcctcct
     9061 cctctcggga agggtcattg accggatcgg gaccaaattg ggttttgcga ttacgatcac
     9121 ggtctggagc atcgcgggca tgttgcatgc cctggcaaga agcgtttgga gtttttcatt
     9181 ggttcgcatc atcctgggtt tgggtgaggc cggcaatttc cccgcggctg tcaaaaccgt
     9241 ggcggaatgg tttcctaaaa atgaaagggg tctggccacc ggaatattca attcgggcac
     9301 cagcgtgggc gtggtgctca gtgtgttgct cgtgccgctc attttgagtt tcggaagctg
     9361 gcagcaggtc ttttggatta cgggtgccct cggttttata tggctggccg cctggtgggt
     9421 cttttatgag gtgccttcaa aacagaaacg attaacggct gaagaatatg agtggatccg
     9481 tagcggacag gaagaagcag cgcaggtgca ggtagccatc cgctggaaaa aattatttgc
     9541 ctttcgccag acctgggcca tgatcaccgg taaaggcctc attgacccga tcttctggtt
     9601 ttttcttttc tggctgccct cttatttctc ttccactttc agccttgatt tgaaaaagcc
     9661 gagcctggaa ctcatgatca tttacacggc gacaacactg ggcagcattg gaggcggtta
     9721 tctttcatcc ttgttgatta aaaagggctg gccggttctg aaagcaagga aaacggtgct
     9781 gttgattttt gcttttaccg aattatcggt ctttttcacc cagtttgcca acgatgcctg
     9841 ggtggccgtc ctattgatca gcctggcggt ggccgtgcac caggcctggg ccactaacat
     9901 tttcaccctt gccaccgacc tgtttccaaa ggaagcggta agctccgtgg cgggcatcgc
     9961 gggcatggca ggggcagtcg gcggcatatt ttttcctttg ctggtgggat atatcctgga
    10021 ccggtttaaa gcggtggacc atctgaccgg cggttaccat ttgatcttct ccatctgcgc
    10081 ctttacctat ctgttagcct ggctgatcat tcatttgctg acccggaaat cggggagggt
    10141 ggaactggcg gagcttatac ctaagggata aggttttttg caaaaaggaa atattcaact
    10201 cattcaatca aatcctaccg attctatcgc cggtgggatg cgaaatcaac agcagtggac
    10261 gtaacgttcc ccggcatttg ccaaatggga attgtacgga aaatatacgt acttttgtat
    10321 caaaggaaat tcatggcctc cgcaaaacaa gcgatgacaa gatttgacac caggcttacc
    10381 aaggagcaaa agttgttgtt caaaagggcg gctatgcttg gtggctatcg caatttatcg
    10441 gattttgtga tcaccacggt tcagaaccgg gcggtcgaaa tcattgaaac taaagaaaga
    10501 atcatcgcat cccagaggga tttggatatc ttttttgagg ccatcacccg gcccgcaaaa
    10561 cccaacaaag ccttgcggtc agccgcaaga ggtttcaaat ccggcattcg aagtggacac
    10621 tgaaaccgtt ctcctagggt caaggcaccg gaagacggat tttttgtgtg gcaaggtgca
    10681 gctggatcat tatctccacc accaggctgg ccaggatgtc aaacgaaaat tggccgcatg
    10741 ttttgtgtcc gttgatccgg taaatgaacg cataagagga tattatacct tatccaatta
    10801 ttctttgcct ttggctgggt ggccggaaca aataaaaaag aaattaccga aatcctattt
    10861 agccataccg gttaccttgc tcggcaggtt ggctgttgat cacagatacc aggggcaggg
    10921 attgggtgaa tggttattac tggatgccct ataccgaagt tatacttctt ctgaaatcat
    10981 agggtcctta ggggtcgtca cagatcccct ggatattgaa gccgaaaatt tttatactaa
    11041 atacggtttt attaaactgc ctgacagcgg caaaatgttc attgcgatgc aaaccattat
    11101 tcaactcttt caataaggaa taaccgctac catagccatg cctggaatta gagacggcaa
    11161 aaaaactgaa gggaaggcac aggttgctga aaggaaataa ggtcgtttga ttgccatata
    11221 gaccattgag gggtgtatga caatcctgcg caggcgggag caggcttcca ggtcttgtta
    11281 tcgaacaaaa aagacctgca ttttgccata ccccccaggg aggggtgtaa gacaaattgc
    11341 attaagcggc tttttgtatc aaactcaaaa cacaagccca gttattgctt tttttcatag
    11401 ctctcaggtt tgcaatggat tgggctcctt taatggacca tctttgacca gataatttta
    11461 atctttgttg gactacattg cgatgagcag attcgatggg tcctgaacct atcgataagc
    11521 cgctatccag ataactttta tactgcattc tatcctcatg ttcttcataa tatcgaataa
    11581 cttcttggag ggtttgtttt atttctggtt ttttagggcg gtacttcttc aattcctcaa
    11641 ctacttcatc cactttgttt tcaagtaatt ttttcttctg cttttccatc catattcgac
    11701 gatgtacttc aatcgggaac tgacccagac taaatcgagc cagcttttca atccccatgg
    11761 tctgttttaa aatctcctct ccctcttcaa acacatatcc ttgccctacc agactcatga
    11821 ggtcttgtaa cctttcactg attccaaaaa cctttacgtt tgcgcttaat acatgctgct
    11881 tacttacctc gacggctcct agacaagttt gaagtttttt tttcattcct gctcctaggc
    11941 aacggaccaa tggactcctt cagaatcttc tggctgattc gctgacccat ttccaaaatc
    12001 ttcgtctcgt actcatagcc gctagttatc gttccttctt gtgcaaacca ctcggctaat
    12061 tcttgtttta ctgtttctag gatagaggcg gttgcttcta tctttttcat ggtgggaaat
    12121 attataccct ctaatttaac acttctgtgc attttgtcct acacccccag ggagaaaccg
    12181 gttcctccca ttcatgaaat ttcctatatt gcataaagac tttttcaacc ttccatttaa
    12241 ataccaatcc gatatgaaac ggcgtaatct tctcaaaaac ctcgcagcgg cttccgcggc
    12301 ggcgaccctc ccccttcagc aggtactggc caaaaacacg atacccaaaa tgcaggcctc
    12361 cggcgatatc cggatcaccg atatcaagac catcctcacc gctccggacg gcatacgcct
    12421 ggtcgtggtc aaggtgatga cttccgagcc cgggctctat ggcatcggtt gtgccacctt
    12481 tactcaaagg gcctatgtgg tggcgaccgc cattgaaaaa tacctcaagc cttttctgac
    12541 cggccgtaat gtggatgaga tcgaagacat ctggcaatcc tcctatgtga gttcatactg
    12601 gcgcaatggc cccgtcctgt tcaatgccat gtcgggagtc gatatcgcct tatgggatat
    12661 caaagccaaa agggccaata tgcctttgta ccagttattg ggcggcaagg tgcgccacgg
    12721 cgtggacctt tattaccatg cctctggact ggaatactcg gaactggatg acaaagtagc
    12781 cgcggccatt gcaaacggat accgccatgt ccggatccag atgggccaga aaggcaatgc
    12841 aaactatgga gcccgcgggg ctaccggcac gccggccagt tcggtgggtt tgcccccgga
    12901 acaatccggc ccgaccgatc ccaagatcat ttttaacccg gcggtctaca cccgctccgt
    12961 ccccaaaatg tttgaacata tccgggtcaa attcggtgaa gaagtggaac tcctgcatga
    13021 catccacgaa cgcatccctt tgaatgcggc agtgaacatg tgcaaggaac tggaacaata
    13081 ccatccttat tttatcgaag acccctttcc gccggaagac aacgatcatt tcaagatcct
    13141 gcgtcagcaa accacggtac ccctggccat gggagagctc ttcaataccc agcaggaatt
    13201 cctgcccctg atcaaagacc gcctgatcga ttttatccgg gtgcatatct cgcagatcgg
    13261 tggactgagc ccggcgcgca aagtgcaggc cctgagcgaa tttttcgggg tgcgcacggc
    13321 ctggcatggt cccggcgacg cttccccggt cgcccatgct gcccaactgg ccctggaatt
    13381 gtgcagttat aatttcgggg tccacgaagg atatgtcttc ccggaaaaaa ccaaggaggt
    13441 cttccccggt tgcccggaaa cccgcaacgg ctatatgtat gcccaggaaa aaccggggct
    13501 gggcatggat atcgatgaaa aagctgccgc aaaatatcct tttgccgaag ggaaaaactt
    13561 tgactattcc tggggcacca cccggaggcc ggacggcacc gtgatccgtc cgtaaatgat
    13621 ccgtaaatgg gactgcgcct accgttattt gcagccaggg acttgccttt caccctcatc
    13681 aaactttggt tgctggtata cctggtcctg gggatcagag gcaggatctg cggccagcag
    13741 gacccggagg ccggttataa acttattttc aacgggaagg acctcgaggg ttggaaagga
    13801 gatccggtgt actggcgggt cgaagacggg atcctgaccg gagaagtcag cccggccacc
    13861 ttgctgaaga aaaattcatt catcatctgg cagggcggca aacccggtga ttttgagctg
    13921 aagctggaat accgggtgtc ctccaaaggc aacagcggaa tcaattaccg gagtgtgctg
    13981 gtggatgggg aaccctatgc attaaaaggc taccaggccg acatcgacgg ggaagaccaa
    14041 tggagcggcc agaattatga agaacgcggc cgtactttcc tcgccaggcg agggcaacag
    14101 gtccgggtgg atccgggccc ccggataagc ctgaccgcgg aactcggaaa gccgggcgac
    14161 ttgcagcaat atatccgcaa ggaagactgg aacgaatatc acctgatcgt aaaaggcaac
    14221 cacctccggc attttatcaa cggcaaactg atgagcgagg tgctggatct ggacacgata
    14281 aaccggacct ccgaaggcta cctgggagtg caggtgcatg tggggccgcc aatgaaaatc
    14341 gaattcagga acatccgctt aaaacccctg aatccttaaa tccaatgaag ccggacagtt
    14401 caaaatgcct gtattaaaac cttgaattat gtttcaaacc aattcattgt gggtcgagat
    14461 agccgtggtg agcatcttct atttattggg ccatatcttc atgggccact ttgcagaaag
    14521 ggatcccaag tggagaaaat tcgccaaata cctggtcacc ctggtcctgg tcatgcttct
    14581 atccacctat ttcggccgat cggtctccat gactgttttg ggactgtccc tcctccccat
    14641 actgtatatt cacgcggtca tcctgccccg caaaggcatc aacggctgga ccggggaacc
    14701 gaaatcgagg tattatgatt tcaggggttg gaacaaggat attttcggga caggcagaac
    14761 tcccggtaat ccggaaggat gaggtgattt cccctttatt aaactatcca agtccttctt
    14821 atgggaaaaa gggttaaatg gcgaggcccc gactttgttc caaactacca atcccgcagg
    14881 gattaaaagt aacgaatcgg agcgcagccc ccgggattta tagcccagcc ttccgaccct
    14941 gtcagggtcg aatctagacc tttacccact tctgagtccg gtgactttct atgatccggt
    15001 cacacaagag gagttcgcgc catccttcct caaaagtcgg gaagctggga tgttccggtt
    15061 gtttgccttc agccaccgct ttataaattt cggtgaagag ttgtttggag gtgctgccga
    15121 atccttcgtt atggccgccg ggataggaga tgatgctgcg tacggattta tccaccaggg
    15181 aaggatcgcg gagcatgacc gcgttggctt cgttgcggtt gccgatccac agttcattgg
    15241 gggtctccga acaccaggca agggtttgtt tggagccggc gatctccagg ttgatccggt
    15301 ttttccttcc ggccgaaacc tggctgacgg tgaccacgcc cttgcagccg tcgttgaacc
    15361 tcaacaggac gctggcataa tcttccgtcg caatcggcac gtcgctgtaa tcttccgggg
    15421 tcaggagttt tcccgaatag gtttccaccg gtttaagcgg ttttttacgg atgggatgga
    15481 tggttgaaaa atcggccatc acttccacga tgctgagtcc ggaaatatat tcgatcaggt
    15541 caatcaggtg ggatccgata tccgcaatgg ccttgctttc cccggattta tcgggttcca
    15601 gccgccagtt gtagtccgtt tcgaaaaaaa gccagtcctg gaggtaagat ccgatgatgg
    15661 aatagatccg gccgatttcc cctttttccc gcatggcttt catctggtgc accaggggat
    15721 aataacggat attaaaactg accgcgttga ccgccttttt ttctttggca agggcgatca
    15781 attcttcggc ttcttttacg gtcgttgcca ggggtttctc acaaaccacg tgtttgccgg
    15841 ccagcagggc ggctttggcc atcgaaaagt gcaggttgtt gggcgtgcag atatgaatgc
    15901 agtcaaaatc tgcttcccgg atcatctggt catagtccgt ataataagcg gggacgccca
    15961 gggctgccgc cttatccttt gcctcattct cgatggggtg gaacatggct accacatcca
    16021 gggtgccaac ccttctgagg gattcgatat gtacgggtcc gataaaaccc acgccggcga
    16081 cggccacttt tatttttttt gtcatgatgg acgaaatgaa ggttaaataa gatttgaagg
    16141 ggccaattta tcaataaagg atgggatttt ttacaccgta tgtatccggt ttgatctaaa
    16201 aagcctgcag cttccagcca gtgaggtcaa gcactcaatt aaaggccggc atcgtataat
    16261 tttttacggc gtcatccacc accagcaccc aatcgtggcc ataccctgaa gaaggcggga
    16321 taaactcctg ggagatctta ttttcgaaag tgcccagggc ctggctttga ccgtttttgg
    16381 gattatacca gtgtgcagtc agttgtgtcg cggaaagttt cccggtgtat aaagtgaacg
    16441 tatttccctg ggcggaataa acaataatat aatccttgcc ccggctggcc tgcaggcggt
    16501 cgttattgcc tccatgggtg gaaaggaggg tctggtcggg aatgcggtca aacatgggcc
    16561 tggactcaaa gaggtaacgg agatatttca tctggttggc tccgggcaag tccagggcca
    16621 cataccaggg atgatgaggg ccattgaccg gggtgcggtg cggggcgtac atctgccaga
    16681 tgtcgtggca gccgtaagta tggccaaagg ctccggccag cacggtgtac caggccgaac
    16741 gccggacatc gtaagcgctg gaagtcccgt tttccctcgc gttgaaacag accgggtggt
    16801 cttcatagat gggttccccg tcgagcaccg gtttcacggg tttcaggttg tagggatatt
    16861 ggatacggtc ccagacattg ttttcacggc aatggccggt ctgaagcata ttgaaatcca
    16921 gccaatcgtc ctgatgaaac catttggcgg agcccccatc ctgcaccgca ttgggttggg
    16981 gatgaaaggt catgagcgcc cggtcaggac cgcctgcgcc ctccgtgatc ccggcagcca
    17041 tggcccgcca gatgttgacg tcggcatcat gtcttggggt gcggtccccg ccgagtatcc
    17101 agatgatatt ctttttattc ttataacgcc tgccgatcca gaggccgtat tgttttgcgt
    17161 tttccgcatt gaagatctcc ggccccgtgc cccaggtgct tttatttaat ttgtcccccc
    17221 aggtgggtaa aagaccgata taaatgccca gggaggcagc cttttcgatg atataatcca
    17281 cgtgctgaaa gtaggcttcc cggggccggg tgggatcgtc atggtccagc gggatttcac
    17341 cataggcatt gggcgtatgc aatccgtcca attcagccag gacgaccgcc tgaatgacat
    17401 taaatccctg gtccgcgcgg gttttgaggt atttgtccgc ttcctcccgg ctcaggcggt
    17461 gaaacaattc ccaggccgta tcccccatcc agaaaaaagg ttttccatct gccgtttgca
    17521 gaaatcgttt gttgtcgctc acccgcaact gggccagtgc ggtcatgctt tgaaaaagca
    17581 agaagcaaat aagtaaaccc tttgccgttt tcataatccg agtctttatt ctgttaaatt
    17641 atcaaacttt gaccctttcc cgtttaccct tccccctcct ttttgctccc ttcgccttta
    17701 acctttcgcc tttaaccttt tttgctccct tcgccattaa ccctacgtat tttgaatccc
    17761 tggcgggatt ctaatctaaa cccagtgttt caatctgcca atccacttat gctcccttcg
    17821 ccattaccca ttcgcccttc accctttatg ctcccttcgc ctttgaccgt tgacctttcg
    17881 cctttaaccc tatgtatatt gaatccctgg cgggattcta atataaaccc agtgtgtcaa
    17941 tctgccaatc cacttttgct cccttcgcct ttaaccctac gtatattgaa tccctggcgg
    18001 gattctaata taaacccagt gtgtcaatct gccaa
//
LOCUS       DAOQXT010000099        17720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3578_length_17720_cov_6.388622, whole genome shotgun sequence.
ACCESSION   DAOQXT010000099 DAOQXT010000000
VERSION     DAOQXT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17720
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3578_length_17720_cov_6.388622"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2231
                     /locus_tag="PKM27_15080"
     CDS             <1..2231
                     /locus_tag="PKM27_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNR08641.1"
                     /translation="VASSGFTAYARHIIGSDFYYDCNGPGRGGNTKNFSFNLTIYRDC
                     SNPAGSPYDPDASFGVYRFNGSRYTYITQFTVNHGQISRVLPENNPCLIIPPNVCVEE
                     ASYRFTVDLPVTSDTYVIYYMRCCRNNTILNINAPNNTGATFYTEISPQAQLACNSSP
                     RFKAFPPIVICADFPLDFDHSATDKDGDSLVYEFCTPLAGGGSGDGDPFGGGQGCGAV
                     RPNPAICAPPFTYVRFVSPLFTTKDPMGPGVLQLDPMTGKLEGKPVNLGQYVVGICVN
                     EYRAGVLIGSIHRDFQFNVGTCEQAVHAKIQSDTSVGKNFSINYCGDNTVKLTNQSFR
                     EEYIKNYYWEFRSTGGGQAHTSNEKNATITFPAPGKYKGLMIVNRNALICSDTAFVDL
                     NIIPSDIKAEFEFDYDKCSSLPIKFMDQSTGRITPPRTWHWDFDDGSSSRNKNSSHLF
                     QVPGKYQVTLTVTDGSICKSTASREIAYFPAPQLLDVLPDKFRGCAPATIRFQNLSIP
                     LDSTYQVEWDFGDGSRSNGIHATHTYESPGVYSISMSIRAPSGCVTRESFNSFVRVQE
                     GPVSDFDFSPKSLTTLNPSVQFTNHSRDAVAYSWNFGDENGSELENPSHLYQDTGSYV
                     VNLIAKHQNGCIDTSQKQLDVLLNISYFLPNAFTPNNDGVNDFFLGTGSMTGMQDFSM
                     SIFNRWGEMVFYTEDPLLGWNGRKNNQGMVEPGGVYVCLVRYKTDRGEARELKGFATL
                     IR"
     gene            2310..2621
                     /gene="rplU"
                     /locus_tag="PKM27_15085"
     CDS             2310..2621
                     /gene="rplU"
                     /locus_tag="PKM27_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNR08642.1"
                     /translation="MYAIVTIAGQQMKVKEGQKLFVHRLDAKQGDTVSFDRILLIDRD
                     GAVEIGTPTLPGTVGATVLADDVQGDKVVIFKKKRRKGYKVKRGHRQQFTQIKIDSIT
                     A"
     gene            2633..2896
                     /gene="rpmA"
                     /locus_tag="PKM27_15090"
     CDS             2633..2896
                     /gene="rpmA"
                     /locus_tag="PKM27_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNR08643.1"
                     /translation="MAHKKGEGKVLNGRDSNPKYLGVKRFGGQVVKAGNIIVRQRGTR
                     FHPGNKVGVGKDFTLFALADGAVQFKKGRNDRTFVHIVETAQA"
     gene            complement(2966..3709)
                     /locus_tag="PKM27_15095"
     CDS             complement(2966..3709)
                     /locus_tag="PKM27_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR08644.1"
                     /translation="MVSFRNVCMLLLLGSLWATQGFAQEGIQEALQQFQDGRYDRAAT
                     IFEKMLREEKASPDLYFNLGTCYLQDGRIGEARLAMEKGLRLAPGHKKLLHQLDLVKS
                     RIDPQIEALPSFLLLHWFAGLRDRWPSSHWGYLMLVLIWLFSLLIFLRRAGIWTAPAA
                     LKYGLIGLAAFSSILYFSRNHAEHRHYGILLEGKPLRIAPEANSQVLLPLGAGSKTEI
                     VDSLGGWYKVILENNDQGWIEKSSLTKVE"
     gene            complement(3712..5535)
                     /locus_tag="PKM27_15100"
     CDS             complement(3712..5535)
                     /locus_tag="PKM27_15100"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNR08645.1"
                     /translation="MYRVPFLFVLNILLLSGIPSDALAQAGAAGEQQIKILHSDYTFL
                     DIEPGLETKILKGDVQLYHDSSFLYCDSARIVDLLVYAYGNVVIRHNDTVQIFADSIR
                     YNGLDKAADLYGEVILVSGSQSLFTRKLHYDLNASVASYHTPATLKSKETVLKSQRGY
                     YFVQEDLAHFAGQVQLVDPEIQLRSDSLQFNTKTQTAYFTGPTLMERQDRTIYCEGGF
                     YDVDDKQARFTGNPQFQEKDKKASSEVMLYDGLNGQITLFNQVKYLSEKETLEGDSLY
                     YNENTRIMTLLGEGMIRTEGGTVRSSKKLVYHDSTGVFITEGRSVLQDSSNYLEADLI
                     EREAGSEDLKARGAVYWTDTLNRVDIHCDSLHFNKRENRVVATGKGRQPLFKSFDNPA
                     DTLFLRADTLVAYQADTLRPDKSIKAYHRVTVFRSDLQARCDSLSYHQKDSVFTLYHD
                     PVIWSDTSQFFADTIRLLQSGNEIKSIYLLQNAMIINSPDLRYFNQIKGRRVRADFDS
                     SELATVDVLGNAEAVYYILDDDRAYIGVNKIICSNILVKFGNNQVDRIYFFTEPNGTM
                     YPMRKADHEALKLKGFKWLDSERPTYTQLVNPGEVNLQDRN"
     gene            5631..6077
                     /locus_tag="PKM27_15105"
     CDS             5631..6077
                     /locus_tag="PKM27_15105"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HNR08646.1"
                     /translation="MIQRIQTVFLLLAVLFLTLQTFTPMVLSKSPEPEGVFQDGRLLV
                     SEYQVSLALLAAAILTSLITIFLFKNRKLQKNLVILSIVLIIACHIAGYFYFFPSGSQ
                     STSTLQAGLKPAVGAALPLFGLILLALAYRFIDKDEKIVKSMDRLR"
     gene            complement(6324..8477)
                     /locus_tag="PKM27_15110"
     CDS             complement(6324..8477)
                     /locus_tag="PKM27_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNR08647.1"
                     /translation="MTNVAAQSWNNKTICSPDFIVVPAGSGASLNVLANDVSMSGSPV
                     YLKSISYSDQVSASIEGGNIRFTSVSNADGIGHLIYTACDAQGHCGVGEVSVLVVDPS
                     RVAYSDTVYHAVAKSSSFRLYLPEKGFQLKVKPAHGLLAKIDDYQYEYHPLGSHGNRD
                     LLVFESGKKRKVFDLTQVDRPVKNQFIQDDIVYLNKNTSRWFAALKNDLANPVITQFT
                     QPDHGQVDLNPDQTFTFTSDFDFEGISRFEYTACNSSGCETASVYLYVSDFLPREDLS
                     PAFRTAEGRSLVIPYQVPIEDYEFRIITPPQFGTLDFYSGPVSINLECEQMEVMHPLI
                     YTPFPGFIGNDRFVVNFCLKTGTKQCAPVLIKVETYKEQTCRPATDFVWPGDANTDGL
                     VDLKDLNTISALIGTEGQAREQGNTTWTNQYAPDWNRATTTGSINAKHADADGDGLVD
                     AEDAKVIIQNYNQSNKIIPQGVYFLDPALSTSSPVAEVIAPGDDAVIQLAFGNQDNLL
                     YDVEAISFDLEYNDELLGEEDILIEVLGNSWFGYDNSLLDAKVFTKGKISVNFASTRG
                     KLKHGAGKTIKVRAKGGPIVSHVEGFKIPKKLPVEFKVTNIQLTQGNGTVISLPGSRS
                     TVTVDFDRKPQDKAEFALYPNPATNWVGIKTLDPDERLEKIMLIGIDGKTILNRKLDP
                     VNQINQSLYHLEPGLYILQAHTSAGVFSKKLEILR"
     gene            8704..10128
                     /locus_tag="PKM27_15115"
     CDS             8704..10128
                     /locus_tag="PKM27_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08648.1"
                     /translation="MKELKFVRVLSQLSKNELNKLQRFLESPYFNVNPKVLGLFKAVV
                     HEGNLDDPDQVNKNRIWKKLMPDQAFHDPRWRKWCNELMGLIEKYLVCEQLDKKPLMA
                     NNLLLESVYENKLTLLYKSILNKAENDLKKNKNPSSEFHLQHFLFQRNLYDLKEYEIN
                     MESQSNLETIHEVLDLFYLAEKTKQLVNAVTRRIDFNLPIRIDLEDQLIQIVRQGAYL
                     EHPEIGLYYKIYQIKNQKDPGPLYPGLKQSVLSHLDKFPQQEAVDIFKEMINFTTLQR
                     NQGRTEFEHESLEWYKYGLKYDLVFPNGAFHPGHFLNIVITGIRTKEFAWTEKFIEEY
                     QNIIPKDQKNTLVTFSLARLYFNQNQFGLVIQKLQDVEFDELTYNLDSKVLLLATYYE
                     VEEWPAMSSLAESFRTFLNRHQKDMTPAKKLRYSNFIKYIKRLSRVKYQDRAARLRAL
                     EEIKSTTAVVNQGWLLEKAKAISR"
     gene            complement(10137..11315)
                     /locus_tag="PKM27_15120"
     CDS             complement(10137..11315)
                     /locus_tag="PKM27_15120"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNR08649.1"
                     /translation="MANALDFSNTEIAFAGKTDQELKETLRLFKLMKNPNLVKLGSGL
                     AMLMVRLKLPLMDPLIKATVFRQFCGGTNLLDCQTTIDRLYKNKTFSVLDYGAEAKDS
                     EKDFDHTLNEILKTVQFAAANASVPVVSLKISSLARNAFLEKWQGKNPLDASEEAELA
                     KIRKRLNRILHLASDMGVGVFIDAEESWVQDAMDTLVEELMEAYNKKKVVAYNTYQMY
                     RTDRLEVLKKAFEKAQNRNYYLGAKLVRGAYMEKERKRAADRAYPSPIHATKEETDEA
                     FNQAIRFCIEHYNRISSCNASHNMDSCQLMAKLITDKLIPRNHSHLNFCQLLGMSDNI
                     TFNLAYHGFNVAKYVPYGPIVEVVPFLIRRAEENTSITGEVGRELALIREEVARRGMK
                     "
     gene            complement(11321..12055)
                     /locus_tag="PKM27_15125"
     CDS             complement(11321..12055)
                     /locus_tag="PKM27_15125"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNR08650.1"
                     /translation="MRKFGLIGYPLTHSFSKKYFADKFIREKIEDAVYELYPIPQIEE
                     FPALVAGIGPGLMGLNVTIPYKRSVIPFLDALSPAARAIGAVNTIHFVNGLTTGHNTD
                     ATGFEESLRSWYDAGTGRALVLGDGGSAQAVKYVLQKLGIPFHTVSRQKKEGTLSYEE
                     LDHTRVGNCTLIVNTTPLGMYPDVKSCPPIDYSALGKGHFLYDLVYNPEKTAFLAKGE
                     ARGCRTMNGLDMLHRQAEAAWHIWNS"
     gene            12146..13573
                     /gene="miaB"
                     /locus_tag="PKM27_15130"
     CDS             12146..13573
                     /gene="miaB"
                     /locus_tag="PKM27_15130"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNR08651.1"
                     /translation="MAETGNTQELMDELRQGEVYAGQSEIPSGLKYYIESYGCQMNFS
                     DSEIVASILESEGYGATREVEQADLVLLNTCSIREKAEETIRKRLRTFDQLKPARPGM
                     LIGILGCMAERLKSQLLEEEKLVDLVVGPDAYRSIPGLIAGAHDGRKGLNVFLSREET
                     YADINPVRLDSNGVTAFISIMRGCDNMCSFCVVPYTRGRERSRDAYSIVAEASTLFEK
                     GFREVTLLGQNVDSYKWKNPENGQVVSFANLLRSVAQIHPDLRVRFSTSHPKDITDEV
                     LHTMAAHENICKYIHLPVQSGNNRILDLMNRTYDRGWYIERLEAIRTILPECAVSSDV
                     IAGFCSETQEEHQDTLSMMEYARYSMSYMFSYSERPGTPAARKLADDVPLETKKSRLA
                     EIVALQNRLSLEHNRKDIGRIFKVLIEGDSKKSKEEFKGRNTQNKMVVFPKVSAYRPG
                     DYVLVEIMDANSATLFGKIINTPPQ"
     gene            13570..14856
                     /locus_tag="PKM27_15135"
     CDS             13570..14856
                     /locus_tag="PKM27_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNR08652.1"
                     /translation="MTQFSVQTIKQRFGIIGNSKDLERALEIAVRVADTDLTVLITGE
                     SGTGKEIFSQIIHSLSKRKHNKFIAVNCGAIPPGTINSELFGHEKGSFTGATGERKGY
                     FETADGGTIFLDEIAEMPLDTQAYLLRILESGEFFRVGSSKVLRTNVRVLAATNVDLI
                     ERIHRDRFREDLYYRLNIVPIHVPSLKERREDIYLLFRKFSVDFAEKYGTTPVELDEE
                     ARDILTQYSWPGNVRELKNFAEQVSVLSESRLITRDILMQHMPGSQSGQLSLVTGRAG
                     DGRIDEREILYKILFDMKKDMNELKTLFFELIRRNNLEMPELIAPVQEKAWVPDSGTW
                     REKAYAIPAGSAADASAAVEGIARPVILEKIEPKVTLSPKSIVVEEPLSLEQMEKDLI
                     GKALNKHKGRRKEAARELGISERTLYRKIKQYNLDD"
     gene            14915..15385
                     /gene="lptE"
                     /locus_tag="PKM27_15140"
     CDS             14915..15385
                     /gene="lptE"
                     /locus_tag="PKM27_15140"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNR08653.1"
                     /translation="MGFFCAQVSCYSFKSTSIDPSITSFTVLPFENRADNGPVTLAIS
                     FSEKLKDKVRRESRLKFVETDPDITFSGNIRDFSVVSQAPTADNTSALSRLTISVQVS
                     FENRRDETKNWNQSFSYFQEFDGRTNLLDIQESLVERINNQLVEDIFNKAFGDW"
     gene            15417..16064
                     /locus_tag="PKM27_15145"
     CDS             15417..16064
                     /locus_tag="PKM27_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08654.1"
                     /translation="MEDLIYYLQHPESLDQLTLEEINRLITAYPYLESLKRIYDKKQG
                     NGTTWSADMDLHSRLDLAAVRAYRQGQDGTALEPPVFDLPAVHEVTLNGLNGHEMQPD
                     SVTVEVPVQPAFQLYPYEASGFIRFLETLPPCTPEPPVTSSTPSGPLVEVDRLIEDSL
                     ELKATTGSESLADLWATQGQYREAISLYERLSFEYPEKSATFAAKIEKLKAENSL"
     gene            16061..16315
                     /gene="secG"
                     /locus_tag="PKM27_15150"
     CDS             16061..16315
                     /gene="secG"
                     /locus_tag="PKM27_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNR08655.1"
                     /translation="MITFLIILIGIVSALLILAVLIQNPKGGGIDSTFGGQGANQLFG
                     VARSTDFIEKLTWGLIGALFVLCIITAMFVATPEATNITQ"
     gene            16493..16756
                     /locus_tag="PKM27_15155"
     CDS             16493..16756
                     /locus_tag="PKM27_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNR08656.1"
                     /translation="MKPINDRVIVKPAPAEEKTKGGIIIPDTAKEKPQRGEVIAVGPG
                     KDGNLMTVAVGDIVLYGKYSGQELHHDGQDYLIMREDDILVIL"
     gene            16808..>17720
                     /gene="groEL"
                     /locus_tag="PKM27_15160"
     CDS             16808..>17720
                     /gene="groEL"
                     /locus_tag="PKM27_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNR08657.1"
                     /translation="MAKQISFNADARERLKAGIDAMANAVKVTLGPKGRNVVIQKSFG
                     APLITKDGVTVAKEVELEDAVQNMGAQMLKEVASKTADLAGDGTTTATVLAQAMVTAG
                     MKYVAAGANPMDLKRGIEKGVRHIVNELKNQTEVIGDDFNKIQQVASISSNNDEEIGK
                     LIADAMKRVSKDGVITVEEAKGTDTYVEEVLGMQFDRGYLSPYFVTNAEDMTAEYENP
                     YILIHDKKISNMQEIVPLLEKVISTGRPILLIAEDMESQALGVLVVNRLRANLKVVAV
                     KAPGFGDRRKAMLEDIAILTGGKMISED"
BASE COUNT         4526 a         4591 c         4425 g         4178 t
ORIGIN      
        1 tggtcgcatc cagcgggttt acggcctatg cccggcatat tatcggcagt gatttttatt
       61 acgattgcaa tgggcccggc agagggggca acaccaaaaa tttcagtttt aacctgacca
      121 tttaccggga ctgcagcaat cctgccggtt ccccttacga tcccgatgcc agcttcgggg
      181 tttaccgttt caacggcagc cgttacacct atatcaccca atttaccgta aaccatgggc
      241 agatcagccg cgtattgcct gagaacaacc cctgcctgat cattcctccc aatgtctgcg
      301 tggaggaagc ctcctaccgt tttacagtgg atttaccggt gaccagcgac acctatgtga
      361 tctattatat gagatgttgc cgcaacaata ccatcctcaa catcaatgcg cccaacaata
      421 ccggagccac tttttacacg gagatcagcc cccaggccca gctggcctgc aacagcagtc
      481 ccaggtttaa agcctttccc cccatcgtga tctgtgccga ttttcccctt gattttgacc
      541 acagcgctac cgataaggat ggcgattcgc tggtctacga attctgcacg cccctcgccg
      601 gagggggatc tggagacggg gacccgtttg gcgggggcca gggttgcggc gcggtacgtc
      661 ccaatcccgc catttgtgct cctccattta cctacgtccg gtttgtctcg cccctcttca
      721 ccaccaagga tcccatgggt cccggagtat tacaactgga cccgatgacc ggcaaactgg
      781 aaggcaagcc ggtcaacctc ggtcagtatg tagtgggcat ctgtgtcaac gaataccggg
      841 cgggtgtcct gatcggctcg atccaccgcg atttccagtt caatgtaggc acctgtgagc
      901 aggcagtgca tgccaagatt cagtccgaca ccagtgttgg aaaaaacttt tcaatcaact
      961 attgcggcga caataccgtc aaattgacca accagagttt ccgagaggaa tacattaaaa
     1021 attattattg ggaattccgt tcgacgggag gaggccaggc tcacaccagc aacgaaaaaa
     1081 acgccacgat caccttccct gccccgggca aatacaaagg gctgatgatc gtcaaccgga
     1141 acgccctgat ctgcagcgat acggcctttg tggatttgaa catcatccct tcagatatca
     1201 aggcggaatt cgaatttgat tatgacaaat gttccagcct gcccattaaa ttcatggacc
     1261 agtccaccgg ccggatcacc cctccccgga cctggcattg ggactttgat gacggctcct
     1321 ccagccgcaa caaaaacagt tcgcatcttt tccaggtccc cgggaaatac caggtcaccc
     1381 ttacggtcac tgatggaagc atatgcaagt ccacggcaag ccgggagatc gcttatttcc
     1441 cggcgcccca attgctcgat gtgcttccgg ataaattcag gggctgcgca ccggccacga
     1501 tccggtttca aaacctgtcc atcccgctgg attctaccta ccaggtggaa tgggacttcg
     1561 gcgatggcag ccggtcaaac gggatacacg ccacccatac ctacgaatcc cccggggtat
     1621 attcgatatc catgtctatc cgcgctccca gcggttgtgt gacccgggaa tcgttcaact
     1681 cctttgtccg ggtgcaggaa gggccagtga gtgacttcga tttttcgccc aagagcctca
     1741 cgaccctcaa tcccagcgtt cagtttacca accattccag ggatgcggtg gcctattcct
     1801 ggaatttcgg ggacgaaaac ggttccgaac tggaaaatcc aagccatctc taccaggaca
     1861 ccgggtccta tgtcgtgaac cttatcgcca aacaccagaa tggctgcatc gatacttccc
     1921 aaaaacagct tgatgtcctt ctgaacatca gttatttcct gcccaatgcc tttacgccca
     1981 ataacgatgg agtgaatgac ttcttcctgg ggacgggctc catgaccggc atgcaggatt
     2041 tcagcatgtc cattttcaac cgctggggtg aaatggtctt ttataccgaa gatcctttgc
     2101 tgggatggaa cgggagaaaa aacaaccagg gcatggtgga acccggcggg gtatacgtat
     2161 gtctggtccg gtacaaaacc gaccgggggg aagccaggga actcaagggt tttgccaccc
     2221 tgatccggtg atttccggtt tggattaaaa tttgataatc tcgccggatt accttatttt
     2281 tgcgcttcca aaaaaagaaa ataaaagata tgtacgcaat tgtaactatt gccggtcagc
     2341 aaatgaaagt gaaggaaggc cagaagcttt tcgtccaccg gctggatgca aaacagggcg
     2401 atacggtctc ttttgatcgg atcctgctca ttgaccgcga cggcgctgta gaaattggca
     2461 cccccaccct tcccggcacc gtcggggcca cggtattggc cgatgatgtc cagggagaca
     2521 aggtggtcat cttcaaaaag aaaagaagaa aaggctacaa ggtcaaacgg ggccaccgcc
     2581 agcaattcac ccagattaaa attgattcaa tcaccgctta aaaccaggaa tcatggcaca
     2641 taagaaaggc gaaggcaaag tattaaacgg cagggacagc aatcctaaat atttaggggt
     2701 aaaacggttc ggcggacagg tggtgaaggc gggcaacatc attgttcgtc aaaggggcac
     2761 ccgtttccac ccgggcaaca aggtcggcgt tggcaaggac ttcaccctct ttgctttggc
     2821 ggacggggcc gtccagttta aaaaaggcag aaacgaccgc accttcgtgc atatcgtaga
     2881 aaccgcccag gcttaaacaa acggggcctt taaaaagccg gcccgtatgg gcgaatgaac
     2941 ggttcaagga tcttacttcc ctgaattatt ccactttcgt taaggaggat ttttcaatcc
     3001 aaccctggtc gttattttcc aggatgactt tataccagcc tcccaggctg tccacgattt
     3061 cggttttgct ccctgcccca agcggtagca atacctggct gttggcttcg ggggcgatcc
     3121 tcaggggctt tccttccaga agaataccat aatgacggtg ctcggcatga ttccttgaaa
     3181 aataaaggat ggagctaaag gccgcaaggc cgatgagacc gtacttcagg gccgcagggg
     3241 cagtccaaat ccctgcccgg cgcaggaaaa taaggaggga gaaaagccag atcaggacca
     3301 gcatcaggta accccaatga gaggacggcc atcggtcccg caatccggca aaccaatgca
     3361 aaagcagaaa ggaaggcaag gcttcgattt gcgggtctat cctgcttttc accaggtcca
     3421 gctggtgcaa taatttttta tgtcccgggg ccaggcgcaa tcctttctcc atggccagcc
     3481 gggcctcccc gatgcggcca tcctgcaggt aacaggtgcc caggttgaaa tataaatccg
     3541 gtgatgcctt ttcttcccgg agcattttct caaaaatggt cgcagcccgg tcgtatcgcc
     3601 cgtcctgaaa ttgttgcagg gcttcctgga tcccttcctg tgcaaaccct tgagtggccc
     3661 ataaggaacc caataaaagc agcatacaga cattccggaa agagaccatg ttcaattacg
     3721 atcctgcaaa tttacttctc ccggattaac cagttgcgtg tacgtgggcc tttccgaatc
     3781 cagccattta aaacctttta gtttgagggc ttcgtgatcc gctttccgca tgggatacat
     3841 ggtcccgttc ggttcggtga aaaaatagat gcggtccacc tggttattgc caaatttcac
     3901 caggatattg ctgcagatga tcttgttgac cccgatatag gcccggtcat catccaggat
     3961 ataatatacc gcttccgcat tgcccaatac atccaccgtg gccaattcgc tggaatcaaa
     4021 atccgcccgg acgcggcgtc ccttgatctg attgaaataa cggagatcgg gtgaattgat
     4081 gatcatggca ttctgcagca ggtatatgga tttgatctcg ttgcccgatt gcagcaggcg
     4141 gatggtatcc gcaaaaaact gggaagtgtc agaccagatt accggatcat ggtaaagcgt
     4201 aaaaacggag tccttctggt gataactcag ggaatcgcac cgggcctgga gatctgaccg
     4261 gaatacggtc acccggtggt aagcctttat ggatttgtcc ggacggaggg tatccgcctg
     4321 ataagccacc agggtgtccg cccggaggaa gagggtgtcg gctggattgt caaaggattt
     4381 gaacaggggt tgtcggcctt tgcccgtggc caccacccgg ttttcccttt tgttgaaatg
     4441 aagggaatca caatggatgt ccacccggtt cagggtatct gtccagtaca ccgcacctct
     4501 tgccttcaga tcttccgaac cggcttccct ttcaataaga tcggcttcca gataattgct
     4561 gctgtcctgt aaaaccgacc gcccttcggt gatgaatacg ccggtgctgt catgataaac
     4621 cagtttttta gaagagcgga ccgtaccgcc ctccgtcctg atcattcctt cgcccaaaag
     4681 ggtcatgatc cgtgtatttt cattgtaata aaggctgtcc ccttccaggg tttccttttc
     4741 gctcaggtat ttcacctggt tgaaaagggt gatttgcccg tttaacccat cataaagcat
     4801 cacttccgag ctcgcttttt tatccttttc ctgaaactgg ggatttccgg tgaaccgggc
     4861 ctgtttgtca tccacatcat aaaagccgcc ttcacaatag atggtccggt cctgcctttc
     4921 catcagggta ggcccggtaa aataagcggt ctgcgttttg gtattgaatt gcagggaatc
     4981 gctgcgcaac tgtatttccg gatcgacaag ctgcacctgt cccgcaaaat gggccaggtc
     5041 ttcctgaaca aaataataac ctcgctgact tttgagtaca gtctccttgg atttcagggt
     5101 agcaggcgta tggtaggaag ccaccgaagc attgaggtca taatgcaatt tgcgcgtaaa
     5161 cagggattgg ctgccgctca ccagaatgac ttccccgtaa agatccgcgg ccttgtccag
     5221 gccattgtag cgaatggaat ccgcgaagat ctgtaccgta tcgttatgcc ggatgactac
     5281 attgccgtag gcataaacca ggaggtccac gatgcgtgcg gaatcacaat acaaaaaact
     5341 gctgtcgtga tacaactgga cgtcgccttt caggatttta gtctcgagcc ccggttcgat
     5401 atccagaaaa gtataatcgg aatgcaggat cttgatctgc tgctccccgg cggcgcccgc
     5461 ctgggccaag gcatcggaag gaataccgga caacaggagg atattcaaaa caaataagaa
     5521 aggtacccgg tacatgcagt caataaacga agcccaaaga taaaaagttg cggattgcta
     5581 ccggcgccgg gccaaaccct ctatcttcgc aggcgattaa tccatcaccg atgattcaac
     5641 ggattcagac cgtatttctc cttttggcag tcctgttttt gactttgcag accttcacgc
     5701 ctatggttct atccaagtca ccggaaccgg aaggggtctt tcaggacggc cggctcctgg
     5761 tgagcgaata ccaggtctcc ctggcactcc tggccgccgc cattctgacc tccctcatca
     5821 cgatattctt gttcaagaac aggaagctgc agaaaaacct ggtgatcctt tccatagtac
     5881 tgatcatagc ttgtcatatc gccggttact tttacttttt cccctccggg tcgcagtcaa
     5941 cctctaccct gcaggcaggc ttgaaacctg ctgttggcgc tgcgctcccc ttatttggat
     6001 tgatcttgct ggcactcgcc taccgtttca tcgataaaga tgaaaagatc gtaaaatcga
     6061 tggaccggct tcgttaaacc caaatgatgc aatggggttt tgatatgaaa gcctgagata
     6121 tttcgcacgg agttcgcggg gtacacggag ggttaataag tgtgtttcta tggtgccctg
     6181 cagagtaatt ataatcctca gagcttgcac ttctccctga ttttagcatt gatagcatcc
     6241 gcgattccgg gatggccggc catcagtggt tcggtcgctt ctcagttctg acccagaaga
     6301 tttgtcttca atgagcagaa gacctatctg agtatttcca gtttcttgga gaacaccccg
     6361 gcggaagtat gggcctgaag aatgtacagg ccgggttcca ggtgatagag ggattggttg
     6421 atctgattta ccggatccag ttttctgtta agtatggttt tcccgtctat cccgatcagc
     6481 attatttttt caagtctttc atccgggtcc agggttttta ttccgaccca attcgttgcc
     6541 ggattgggat acaaggcaaa ctccgcctta tcctggggtt tacggtcaaa atcaacggtg
     6601 accgtggacc gggaaccggg aagactgatg acggtgccat tgccctgggt caactggata
     6661 ttggtaacct taaattcgac cggtaatttt ttggggatct tgaagccttc cacgtgcgaa
     6721 acgatcgggc cccctttcgc ccttactttt atggtcttgc cggcgccgtg cttcaattta
     6781 ccacgggtgc tggcaaaatt cacactgatc ttgcccttcg tgaatacttt ggcatcgagc
     6841 agggaattgt cataaccgaa ccaggaattg ccaagcactt caatgaggat atcctcttcc
     6901 ccaagcaatt catcgttgta ttccaggtcg aagctgatgg cttccacgtc atagagcagg
     6961 ttgtcctggt tgccaaaggc cagttgaatc accgcgtcat ctcccggtgc gatgacctcg
     7021 gcaaccggtg aggaggtgga cagtgcggga tccaggaaat atacgccctg cgggattatt
     7081 ttattggact ggttgtagtt ttggatgatc acctttgcat cttcagcatc caccaggcca
     7141 tcaccgtcgg catcggcgtg tttggcattg atggagcctg tggtggtcgc cctgttccag
     7201 tcgggagcat actgattggt ccatgtggta ttgccctgtt ccctggcctg tccttccgta
     7261 ccgatcagag cgctgatcgt gttgagatct ttcaaatcca caaggccatc ggtattcgca
     7321 tcgccgggcc atacaaagtc ggtggccgga cggcaggtct gctctttata cgtttccact
     7381 ttgatcaata ccggcgcgca ttgtttggtg ccggttttca gacaaaaatt caccacaaaa
     7441 cggtcgttcc cgataaaacc ggggaaaggc gtatagatca gggggtgcat gacctccatt
     7501 tgttcgcatt ccaggttgat gcttaccggc ccggaataaa aatcaagggt cccaaactgg
     7561 ggaggggtga tgatcctgaa ttcgtaatcc tcgatcggaa cctggtaagg aatgaccagg
     7621 gagcggccct cagcggtccg gaaagcaggg cttaagtctt ccctgggaag aaaatcgctt
     7681 acgtagagat agaccgaagc ggtttcacaa ccggaactgt tgcaggccgt gtattcaaaa
     7741 cggctgatcc cttcaaaatc aaaatcggaa gtaaacgtga aggtttggtc agggttgaga
     7801 tccacctggc cgtggtcggg ctgggtaaac tgggtgatga ccgggttggc gagatcattc
     7861 ttaagggctg caaaccaccg ggaagtgttt ttattcaagt aaacaatatc gtcctggatg
     7921 aactgatttt tcaccggccg gtcaacctgg gtaaggtcga ataccttgcg ttttttaccc
     7981 gattcgaaaa ccagcaggtc acggttccca tgactgccca ggggatggta ttcgtattgg
     8041 taatcatcga ttttggccag caggccatga gccggtttta ctttgagctg aaagcccttt
     8101 tccggaagat ataaacgaaa ggaactgctt ttggccaccg catggtagac cgtgtcggaa
     8161 taggcgaccc ggctgggatc cacgaccagg acggacactt caccgacccc gcaatggccc
     8221 tgcgcgtcgc aggcggtata tatcaaatgg ccgatcccat cggcattcga aacggaagtg
     8281 aaccggatat tgcctccttc aatggaagcg gaaacctgat cgctgtagga tatggatttc
     8341 agataaaccg gtgaacccga catcgagaca tcattggcca atacgttcag ggaggctccg
     8401 ctgcccgcgg gcactacgat aaaatcgggc gaacaaatcg ttttattatt ccaggattgc
     8461 gccgccacgt ttgtcacagc caggacaacg aataagcaaa tagcaagaaa cggaaaggtt
     8521 cggttcattc tattatatta gcgacgatat aattgagtgg gcaagatagg cataaattgt
     8581 aaatcaaaaa aataaacata tattttttct atatataaaa ttaacacatt caaaaatagt
     8641 gtaatatata aataattaaa aataaaaatt ttttatataa acttcgtttc aatttgttaa
     8701 catatgaagg agctaaagtt tgtccgggtt ttgtcccagc tctcgaaaaa cgagctcaac
     8761 aaacttcagc gttttttgga atccccttat tttaatgtta accccaaagt gctcgggctt
     8821 tttaaagctg tagtgcacga gggcaacctc gacgacccgg accaggtaaa taaaaacagg
     8881 atctggaaaa agctgatgcc ggaccaggct tttcacgatc cccggtggcg gaaatggtgc
     8941 aacgagctca tgggtctgat cgaaaaatac ctggtctgcg agcaattgga taaaaaaccc
     9001 ttgatggcca acaacctcct gctcgaatcc gtatatgaaa acaaactgac cctcctctat
     9061 aaatccatcc tgaacaaggc agaaaatgat ttgaaaaaaa ataaaaatcc ctcttcggaa
     9121 tttcatcttc agcattttct tttccagcgg aacctctatg acctgaagga atacgagatt
     9181 aacatggaat cgcagtccaa cctggagacc attcatgaag tccttgatct tttttacctc
     9241 gccgaaaaaa ccaaacagct cgtaaatgcc gtcacccgaa ggattgactt taacctgccc
     9301 atccggattg acctggaaga ccaattgatt caaattgtga ggcagggagc ctatctcgaa
     9361 catcctgaaa tcgggctcta ttataaaatt taccagatca aaaaccagaa ggacccgggt
     9421 cccctctatc ccggcctcaa acaatccgtt ttgagccatc tggataaatt tccccagcag
     9481 gaagccgtgg acatcttcaa ggaaatgatc aatttcacca ccctgcagcg caaccagggc
     9541 cgcaccgaat ttgaacacga atccctggaa tggtataaat acgggcttaa atacgacctg
     9601 gtcttcccca acggcgcatt ccatccggga cacttcctca acatcgtgat cacgggcatc
     9661 cgcaccaagg aatttgcctg gacggaaaag tttatcgagg aataccaaaa catcattccg
     9721 aaagatcaga aaaacaccct ggtcaccttc tccctggcgc ggctgtattt caaccaaaac
     9781 cagtttgggc tcgtcatcca aaaactgcag gatgtcgaat ttgacgaact gacctataac
     9841 ctggactcaa aagtgttgct cctggccacc tattatgaag tcgaggaatg gccggcgatg
     9901 agctcgctgg cggaaagctt cagaactttt ctcaacaggc atcagaaaga catgaccccg
     9961 gccaaaaagc tgcggtattc caattttata aaatacatta aacgattaag ccgggtaaaa
    10021 taccaggacc gggcggccag actcagggcc cttgaagaaa tcaaatccac caccgcggtg
    10081 gtcaaccagg gctggttgct ggaaaaagcc aaagccatta gccgctaatc ccggaattat
    10141 ttcattcccc tccgggccac ctcttcgcgg ataagcgcca gttcgcggcc tacctccccg
    10201 gtaatggacg tattctcttc cgccctgcgt atcaggaaag gcacgacttc cacgatcggg
    10261 ccgtagggca catatttggc cacattaaat ccatggtaag ccaggttgaa agtaatgttg
    10321 tcgctcatgc ccagcaactg gcaaaaattc aggtggctgt ggttgcgcgg gatcagttta
    10381 tcggtgatca gtttggccat gagctggcag ctatccatat tgtgtgaagc attgcaggac
    10441 gatatccggt tgtaatgttc gatgcagaac cggatcgcct ggttaaaggc ttcatcggtt
    10501 tcctccttgg tggcatggat gggcgaggga taagcccggt cagccgcccg ttttctttcc
    10561 ttttccatat acgctccgcg caccagtttg gcgccgaggt aataattgcg gttctgcgct
    10621 ttttcgaagg ctttcttcaa cacttccaga cgatcggtgc ggtacatctg gtaggtattg
    10681 taggccacga cctttttctt attgtaggct tccatcaatt cttccaccag ggtgtccatg
    10741 gcatcctgga cccaggactc ttcggcatca ataaaaaccc cgacacccat atccgaagcc
    10801 aggtggagga tgcggttgag ccgcttcctg attttagcca gctcggcttc ttcagaagcg
    10861 tccagggggt ttttaccctg ccatttttca aggaaagcat tccttgccag ggaggatatt
    10921 ttaaggctca ccaccggcac cgaggcatta gctgcagcaa actgaacggt tttgagaatt
    10981 tcgttcaggg tatggtcgaa atccttttcc gaatccttgg cttcggctcc gtaatccaac
    11041 acactgaagg ttttgttttt gtataaccgg tcgatggtgg tctggcagtc cagcagattg
    11101 gtccccccgc aaaactgcct gaacaccgtg gctttgatca acgggtccat caggggcaat
    11161 ttcaaccgga ccatgagcat ggccaggccc gatcccaact taacaaggtt ggggtttttc
    11221 atcagtttaa acaatctcag ggtttctttc aactcctgat cggtcttgcc ggcgaaggca
    11281 atttccgtat ttgaaaaatc cagtgcgttt gccatatttt ttaactgttc cagatgtgcc
    11341 aggctgcttc tgcctggcgg tgaagcatat ccaatccatt catagtcctg caccccctgg
    11401 cttccccttt ggccaggaag gcggttttct ccggattgta gaccaggtca tagaggaaat
    11461 gtcccttgcc cagggcagaa tagtcgatgg gcggacagga tttcacatcc ggatacatgc
    11521 ccaaaggggt ggtgtttaca ataagggtgc aatttcctac ccgcgtatgg tccaattcct
    11581 catagctgag ggtcccctcc tttttctgcc tcgaaacggt atggaaaggg atacccagct
    11641 tttgcaatac gtattttacg gcctgggctg agcccccgtc tcccagaacc agcgcccggc
    11701 ccgttccggc atcgtaccag gaccggaggc tttcttcaaa gcctgtcgcg tcggtgttat
    11761 gccccgtggt taaaccattg acgaaatgga tcgtattgac cgccccgatc gcccgggcgg
    11821 caggggaaag ggcatctagg aaagggatga ccgacctttt atatggaatg gtgacattca
    11881 gccccattag tccggggccg atccctgcca ccagggcagg aaattcttct atctggggta
    11941 tgggatagag ttcgtaaacc gcgtcttcaa tcttttcacg gataaactta tcggcgaaat
    12001 attttttgga aaaagaatgg gtcaggggat aacctatcag gccgaacttc cgcatacagg
    12061 gacacaaaat taatcaatta taaaagaagg ctggtgctgc cattttggca taaaaatcct
    12121 aactttgcgg ctctgaaaat atagcatggc cgaaacggga aacacacaag aactgatgga
    12181 cgagctgagg cagggggaag tctacgccgg ccaaagcgag atcccatccg ggcttaaata
    12241 ttatatcgaa agttacggct gccagatgaa tttttccgat agcgagatcg tggcctccat
    12301 cctggaaagc gaagggtatg gagccacccg ggaagtggaa caggcagacc tcgtattgct
    12361 caatacctgc agcatccgtg aaaaagctga agaaacgatc cgcaaaaggc tgcggacttt
    12421 tgaccagctc aaacccgccc gtccgggcat gttgatcggc atcctgggct gcatggccga
    12481 aaggcttaaa agccaactgc tcgaggagga aaaactggtt gacctggtgg tggggcccga
    12541 tgcctatcgt tccattccgg ggcttattgc cggggctcac gacggccgga aaggactcaa
    12601 tgtctttctg tccagggaag agacttatgc ggacattaac ccggtccggc ttgattccaa
    12661 tggggtcacg gcctttatca gcatcatgcg gggatgtgac aacatgtgtt ctttttgtgt
    12721 cgttccatat acccggggac gggagcgaag ccgggacgcc tacagcattg tcgccgaagc
    12781 aagcaccctg tttgaaaagg gtttccggga agtgaccctc ctgggccaga acgtcgattc
    12841 ctataaatgg aaaaatccgg aaaacggcca ggtcgtttct tttgcaaacc tactccgcag
    12901 cgtggctcaa atccaccccg atctgcgcgt gcgattctcc acctcccacc ccaaagacat
    12961 caccgatgaa gtattgcata ccatggccgc ccatgaaaac atctgcaaat acattcacct
    13021 gccggtgcaa tccggaaaca accggatcct ggacctgatg aacaggacct atgaccgggg
    13081 ctggtacatc gaacgtctcg aagccatacg caccatcctg cccgaatgtg cggtttcctc
    13141 cgatgtgatc gccggttttt gttcggaaac ccaggaggag caccaggata ccttgtccat
    13201 gatggaatat gcccgctaca gcatgtctta catgttttca tattctgaaa ggccgggcac
    13261 cccggctgcc cggaaactgg cggatgacgt ccccctggaa acgaaaaagt caagactggc
    13321 tgaaatcgtc gcgctgcaaa accggctgtc ccttgaacac aaccggaaag atatcggccg
    13381 catatttaaa gtattgatcg aaggcgattc caaaaaatcg aaagaagaat tcaaaggcag
    13441 gaatacccag aataaaatgg tggtattccc gaaggtttcg gcttatcggc ccggggacta
    13501 cgtgctcgtg gaaattatgg atgcaaacag cgctacgctt ttcggtaaga tcataaacac
    13561 ccctccccag tgacccagtt ttcggtacaa accataaaac aacgtttcgg gatcatcggc
    13621 aattccaaag acctggaaag ggccctggaa atcgccgtac gggttgccga caccgacctg
    13681 acggtcctca tcaccggaga aagcgggacc ggtaaggaaa ttttttcaca gatcattcat
    13741 tccctcagca aacggaaaca caataaattc atagccgtca actgcggagc catcccgccg
    13801 ggcacgatca attcggaatt gttcgggcat gaaaaaggct ccttcaccgg ggctaccgga
    13861 gaacggaagg ggtattttga aactgccgac gggggcacca tcttcctgga tgaaattgca
    13921 gaaatgcctt tggacaccca ggcctacctg ctgcgcatcc tggaaagcgg cgaatttttc
    13981 agggtgggat cttccaaagt gttgcgcacc aatgtgcgcg tgctggccgc gaccaatgtg
    14041 gacctcatcg aaaggatcca ccgggaccgc ttcagggaag atttgtatta ccgtctcaat
    14101 atcgttccca ttcacgtgcc ttcgctcaag gaaagaaggg aagacatcta tcttctgttc
    14161 aggaaatttt ccgtggactt tgcggaaaag tatggaacca ccccggtgga attggacgag
    14221 gaggcccggg acatcctgac ccaatactcc tggcctggca atgtgcggga actcaaaaat
    14281 tttgcagaac aggtatccgt actctccgaa agccggctga tcaccaggga cattttaatg
    14341 cagcatatgc cgggcagcca gtcggggcaa ttgtctctgg tcaccggcag ggcgggcgac
    14401 ggccgtattg acgagaggga aatcctttat aaaatccttt ttgacatgaa aaaggatatg
    14461 aacgaactga agaccctttt ctttgaactc atccggagaa acaatcttga aatgccggag
    14521 ctgatcgccc ctgttcagga aaaggcctgg gtccccgatt caggcacctg gagggaaaaa
    14581 gcctatgcca tcccggcagg gtccgctgcg gacgcttcgg ccgccgtgga aggaattgca
    14641 aggcctgtga tccttgaaaa aatagaaccc aaggtgaccc tgagccccaa atccatcgta
    14701 gtggaagagc ctctttccct ggaacagatg gaaaaagacc tcatcggcaa ggccctgaac
    14761 aaacacaagg gccggagaaa ggaagccgcc cgggaactgg gcatcagtga gcgcaccctt
    14821 taccgaaaaa taaaacaata caacctggac gattaaaatg cttaggatat ccgtatcacc
    14881 ccggaagcat ccgcttgttt catccctttt ggtgatggga ttcttttgcg cgcaggtttc
    14941 ctgttattca ttcaaaagca cctccatcga tccctccatc acctcattta ccgtattgcc
    15001 ttttgaaaac agggccgaca acgggccggt cacccttgcc atctcgtttt cagaaaaatt
    15061 aaaagacaag gtacgcaggg agtcccggtt aaaatttgtg gaaaccgatc cggacatcac
    15121 cttttcagga aatatccgtg atttcagcgt ggtttcgcag gctcccaccg ccgacaatac
    15181 ttctgcctta agccgcctga cgatcagtgt gcaggtttcc tttgaaaacc gcagggatga
    15241 aacgaaaaac tggaaccagt ccttcagcta ttttcaggag tttgacggca ggaccaactt
    15301 gctcgacatt caggaaagcc tggtggaaag gatcaacaat caactggtgg aggatatttt
    15361 caataaagcg tttggggatt ggtagccctt aactacattt gtaataagag atgaagatgg
    15421 aggatttgat atattatttg caacatcctg aatctctgga tcagttgacc ctggaggaaa
    15481 tcaaccggct gatcactgcc tatccttacc tggagtccct gaagcggatc tatgacaaaa
    15541 aacaagggaa cgggaccacc tggtctgcgg atatggacct ccattccagg ttggatctcg
    15601 ctgccgtgcg ggcttaccgg caagggcagg acggtaccgc cctggagcct ccggtatttg
    15661 atcttccggc ggtccacgag gttactttaa atgggctcaa cggtcatgaa atgcagccgg
    15721 attcggtgac cgtagaagtc ccggtccagc cggcgttcca actgtaccct tatgaagctt
    15781 cgggttttat ccggtttttg gaaaccctgc ccccctgcac cccggaacct ccggtcacct
    15841 cttcaactcc ttcgggcccc ctggtcgagg ttgatcggct gattgaagac agcctggagc
    15901 tcaaagccac gaccggaagc gaaagccttg ccgacctttg ggcaacgcaa ggccagtacc
    15961 gggaggcgat ttcgctttac gaaagactgt cttttgaata tcctgagaaa agcgctactt
    16021 ttgcagccaa aattgaaaaa ttaaaggctg aaaattcatt atgattacct ttctgattat
    16081 tctcattggg attgtttccg cgctgctcat cctggcagta ttaattcaaa atcccaaagg
    16141 agggggcatt gattccactt tcgggggcca gggcgccaac cagctcttcg gggttgcccg
    16201 ttccaccgac ttcattgaaa agctcacctg ggggctgatc ggggcgcttt ttgtcttgtg
    16261 catcatcacc gcgatgttcg tggccacccc ggaagccacc aacattaccc aataaggttc
    16321 tgccaattct tcagtcggtt tggcctgccc tgtcagccca aacaaaaaaa cactatgttt
    16381 cagtccttca atacagtttc ccggaaaaaa tgtcagttgg ttgtccaatg gcacaataaa
    16441 tgctaagaca gttaaaaata aattgttaaa tacttaataa aatttcaaaa atatgaagcc
    16501 tatcaatgac agagtgattg taaaaccggc accggccgag gaaaaaacca aaggtggtat
    16561 catcatcccg gacactgcga aggagaaacc ccagcgtggc gaagtgatcg ccgtgggccc
    16621 gggcaaggat ggcaatctga tgacggttgc cgtaggggac attgttttgt acggaaaata
    16681 ttccggccag gaattgcacc acgacgggca ggactacctg atcatgcgcg aagatgacat
    16741 cctggtcatt ctttaagtat ctggaaataa gtattcatca tttttcaaag aataaaattt
    16801 atataaaatg gctaaacaaa taagctttaa tgcggatgcc cgggagagac tgaaagcggg
    16861 tatcgatgct atggcaaatg ccgtcaaagt caccctcggt cctaaaggcc gcaatgtcgt
    16921 tatccagaag agtttcggag cccccctgat taccaaggac ggggtcacgg tcgccaaaga
    16981 agtggaactg gaagacgccg tgcagaacat gggagcccaa atgctcaagg aagttgcctc
    17041 caaaaccgct gacctggccg gcgacgggac caccaccgcc acggtactgg cccaggccat
    17101 ggtgactgcc gggatgaaat acgtagctgc cggcgccaat cccatggacc tgaaaagagg
    17161 cattgaaaaa ggagtgcggc atatcgtgaa tgaattaaaa aatcagaccg aagtgatcgg
    17221 ggatgatttt aataaaatcc agcaggtggc ctccatttcc tccaacaacg atgaagaaat
    17281 cggtaagctc attgccgatg ccatgaagcg ggtttccaaa gacggggtga tcacggtgga
    17341 agaagccaaa ggcacggata cctatgtgga agaagtgctg ggtatgcaat ttgaccgcgg
    17401 ttacctgtct ccctatttcg tgaccaacgc cgaagacatg accgccgaat acgaaaatcc
    17461 ctatatcctg atccacgata aaaagatttc caacatgcag gagatcgttc ccctgctgga
    17521 gaaagtgatc agcacaggca ggcccatcct cctcatcgcg gaagatatgg aaagccaggc
    17581 cctgggggta ctcgtggtca accgcctgcg tgccaacctc aaagtggtag ccgtcaaagc
    17641 tcccggcttc ggcgaccgcc gcaaagccat gctggaagac atcgccatcc tcaccggcgg
    17701 caagatgatt tccgaagaca
//
LOCUS       DAOQXT010000100        17518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3625_length_17518_cov_6.945485, whole genome shotgun sequence.
ACCESSION   DAOQXT010000100 DAOQXT010000000
VERSION     DAOQXT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17518
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3625_length_17518_cov_6.945485"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="PKM27_15165"
     CDS             complement(<1..1602)
                     /locus_tag="PKM27_15165"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNR08658.1"
                     /translation="MKTTILPFFLCLVISTNLPAQANVQSLRTQKPVLHARHWLAITG
                     KPLAATAGATLFQQGGNAVDAACAMIAATCTMFDVLSWGGETQALIYNPKTGEVVGIN
                     ALGIAPTGATAQFYKSKGFKYPPATGPLAAVTPGTPGGILVMLAEFGTKSLREVLAPA
                     MQMADGYPMDEDVHRQMKRYADDLKSWPYSKKVFFKGDQLPQPGELFSQPDLYVTLKK
                     MVDTEAAALASGKSRKEAIMAAYDRFYTGDIADEFVRGTREQGGLITKEDLAKWKVLI
                     EKPLSTNYKGIEVYKLREWQQGPVLLQALNMLENLDLKKMRYNSLQYIHTLYQVMNYA
                     YADRDFYYGDPYVPPVEPIQGLLSKAYAAQRLKLIDTAKNDAMALPGDPYPFEGKTNP
                     YLDLIERMKKPSSHRPSDLEEYMKNFYAGTTSVTAADAQGWVVSVVPSGGWMPACIAG
                     NTGIGLSQRMQSFVLDASENPFNVVAPGQRPRVTLTPTLALKDGKPLLAFGVQGGDSQ
                     DQNLLQFFLNMTEFGMNVQQAVEAAN"
     gene            1698..2057
                     /locus_tag="PKM27_15170"
     CDS             1698..2057
                     /locus_tag="PKM27_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08659.1"
                     /translation="MNTKCIECGDKIMGRSDKKFCTDQCRSAYNNRLNSDSNNIMRNI
                     NNTLRKNRRILLEANPEGKGKISREQLVKRGFKFDYFTNLYQTKNGHTYYFCYDQGYL
                     PLDRDWYALVKRQEYIH"
     gene            2081..3655
                     /locus_tag="PKM27_15175"
     CDS             2081..3655
                     /locus_tag="PKM27_15175"
                     /inference="COORDINATES: protein motif:HMM:NF013264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08660.1"
                     /translation="MRSRTSFLSILLLLVSLAIHAQTPNWSETVAKIVYTHCTPCHRS
                     GGSGPFSLMSYGQAFNNRFSMRSAVSQKIMPPWPPDDSYSALAHSRALKEADLEAIVR
                     WVDGGAPSGNLSTAPQAPQYDNLGYIKNPQLVISTPEYTLAGNTDEYRCYPESSNINQ
                     EMWLTALECIPGNPGIVHHILVFRDGGNRSFQLDQQQAGPGYLCFGGAGTSGAELIGA
                     WVPGAQPFVLPKGFGIRLPANSNIIIQVHYPGGSKGKSDKTIVKFQLTDQPQRDAYLV
                     PALNHSTSMTNGPLYIPANTKKTFNQQFALPFNASVIGVAPHMHLIGTSIRTWAVGPD
                     QKEIPMIHIPRWDFHWQGIYQFQKVMKVPAGSMIYSEATYDNTPANHHNPNFPPKDVS
                     VGEATTDEMMITYFLFTLYQNGDEKIIIDSAKALVSSAPDAEIETRPVTVFPNPAGRL
                     SPSRLVFKTDPNDRIELSILDLTGKEIFRKKYLYSAAGTQTIGLGADRLDTGTYIIRV
                     RGQKWQATKKWILTPE"
     gene            3764..4483
                     /locus_tag="PKM27_15180"
     CDS             3764..4483
                     /locus_tag="PKM27_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08661.1"
                     /translation="MDSVETGIKKKFYDKAYWYFMLAALVTIIGFFPSYFSRLTVTDP
                     AHHLHGITATCWMLLLVVQPWLYKTGKLSLHRNLGKSSYVLVPLILLSGLNMVHIMLN
                     NQVNYPPDLPYRLAFIDFITLAQFLLFYGLAINHRKKLQLHARYMACTVLGPLIPALT
                     RALFLIPWIDHFDKALNTSYILIESVLVILLLDDRRTGRIRLPYVLALILFFVQHLVM
                     NFVGQWTWWRSLMDAYAGLRF"
     gene            4566..5831
                     /locus_tag="PKM27_15185"
     CDS             4566..5831
                     /locus_tag="PKM27_15185"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNR08662.1"
                     /translation="MKADLEELVSNKVIDPETARRIREYYDLKSKASKASRLLLILSI
                     TGIVLVGLGLILLIAYNWESLPKAFKLAFAFLPLAATQTFGLWVLVKKGGLVLWHESA
                     ALAILFAVGIAMSLVTQIYHIEGALSGFLQWWIILSAPLVPVFISDVVSLAVWIGTGW
                     YLTSISWTRSDYDLFAPAVILLALGWYSWRLRHEGLKKWMTFHHWVIPIVLTIYIFSL
                     APHTCNRFLLIALLTLFMGFGGVAFTVFKGQGLLTNGFRTGQFLGTWITGIILGFEFY
                     WTSPLQDSLSRCIADNPVFLPLVNLAVLGYLAYALFRRFKESEPDDLGWMGLALIAVV
                     IAGSGYPGFGHIAANIILLAGSARIIYGGLSRENLWLLNLGLLTLASWVISRFFETNI
                     SFVWKGLLFITLGILCFGLNWFILKKQRT"
     gene            5828..6388
                     /locus_tag="PKM27_15190"
     CDS             5828..6388
                     /locus_tag="PKM27_15190"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNR08663.1"
                     /translation="MNTPLIKITGFILLCLFQWILPVSWILQEEKTRTEGIFVKIPCR
                     PFDPADPFRGTFLNIQPLPAEFDYEDSSRFENQQEVWVNYIPDSGEIYRFHSVQPLSE
                     APAQPVHFKVTIRNTYVQGPGAQYTAFLDYPFSKYFISEKEAPRLAEKYNQALSDTTQ
                     TVYAGLFVLDGNASLDGIWIGAEKLE"
     gene            complement(6468..7013)
                     /locus_tag="PKM27_15195"
     CDS             complement(6468..7013)
                     /locus_tag="PKM27_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNR08664.1"
                     /translation="MILHPWHEVDPGDRAPEVCWAFIEISRKSRAKYEIDKKSGLLKL
                     DRVLYSSVMYPANYGFIPRTMHADGDPLDVLVLSQIDITPHCLVEVNIIGVMQMIDQG
                     ESDDKILAVAARDPSVNYICDIADMPPNLEKEIIQFFNTYSVLENKKVTATSFLGRET
                     AFRIINEGIEFYRERAEIGGW"
     gene            complement(7182..8090)
                     /locus_tag="PKM27_15200"
     CDS             complement(7182..8090)
                     /locus_tag="PKM27_15200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNR08665.1"
                     /translation="MNKIIAVCGGSGFIGKAMSVYLTSMGYQVRIITRTKRPGRSFAQ
                     FEWDPAREYLEPGALDGVYGVVQLAGESIEKKRWTASRKKVLYDSRVNSGRFLIKCIN
                     QQVRPPKVYLSMAGIGIFGDRPGELLDESAPPGPSSFISDLSRDWEGVVSGLDPEVRK
                     VILRTSPVLSRGEGILKKFYLPASLGIAPVFGKGEQIYSWIHIEDLVRIFCRMLTRED
                     TAPVYHVTAPAPVSQKEFVTRITRLAGPFILKVPVPRWGLKLALGQLADTLFESLAVM
                     PAKLLEEGFEFRYPDLDSALKHLLKR"
     gene            8154..9890
                     /locus_tag="PKM27_15205"
     CDS             8154..9890
                     /locus_tag="PKM27_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR08666.1"
                     /translation="MPLPPKNAYIPFLLLLSLLACNKARTPLQEGVLQVRLAREPDIL
                     NPVLSATSTAAAIENLLYLPLMDFDPYTQSFKPVLTSGPAEIIRQEDRVGYKINIRQQ
                     AIWDDGSPVTAADVSFTLKATLNPFVQSVGKRSVIYAIDSIEFSGDPRQLVFWTDDRY
                     FLDEYSFTNNFILQAARLDSSGLLEDLSWSELKYMSPEDTGAAAARARDFAASLSRPE
                     FGREGLSGGGPYRLEQWVANQYIKLKRKDQWWGQAFADSAVNFRAYPSEITYRIIPEE
                     ANAITALKEGLLDVLAADISPSNFKSLSQDSSLQTKINFIAGPPIRFVYLSLNNRNPL
                     LDQSETRKALARLLDLDQLQNNLFQGYAQRIEAPFQPAKEHYHHQLAPVPFDIEGAKS
                     LLSQAGWTDSNRNGTVDKKINNRQTELKLRMLVTPGGLSQQVAIHLQEQGRKAGVDIE
                     IIPKPINLLLENLRNHDFEIAALADIQYPGPDDPYPFWHSESYISGGQNYTGFGSPFT
                     DSIINEIRFGSSAPKRIALYKELQEEIYDQQAAIFLFAPQNLIAVNKKWDARAASVRP
                     GYFINDFRLHAR"
     gene            complement(9938..11251)
                     /locus_tag="PKM27_15210"
     CDS             complement(9938..11251)
                     /locus_tag="PKM27_15210"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNR08667.1"
                     /translation="MKHTLRRILTPTLMCAVTALSAQDRGISLFNNTQIYTGRNPALP
                     FPKEFVIGLPEGAGSLVLETGKGPWLIDRADGGKDFSLASGWAGLEKNNYFIDGEWKV
                     RTFFLGKKWKYGQLGLSHEWEGHSSLRFNKDLVGLLGFGNYGYLQVEPRASSQPLDLK
                     SEGEHFIFQAFTLQSALFRNKWSVGAAVRYLGGIQDFHTDILKLKADIRDPLVLKMEE
                     DWSFYSADLVETFSVDSIRLSKSNGLAPGRHPGFAFSIGVQYKAPRWRAALQLKDLGS
                     IRWRKGNRFSRSGNTLYQGIQGIDFLNLNENIFDQVRDTLHALAGVEKSEASFTRSLP
                     ASLLAENQVYFSDRWTVGFSADYIFSRDYGYLMAGAVYRPFPIWSFGGQMSLDTRKDW
                     NLGLLTSLELGVFNLFFATDHVPALWKLNKADRLQARFGLALMWD"
     gene            complement(11248..12747)
                     /locus_tag="PKM27_15215"
     CDS             complement(11248..12747)
                     /locus_tag="PKM27_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08668.1"
                     /translation="MKGIRTILVWLLCAGFACNLPEYYLDRDREEWALPLLNGEIKAM
                     TLLENSVTLGEIRVSQDGTLAIHYTGEILQKTKKDIFQPLPGNLPIPMTDTLNEVTLP
                     VVNNMIIRRAVLGKGNYSFAFGHQETEPVSLQLWIPEMVYQGQTVQHKLEIPYAGETN
                     TSGISGPHPLEDIRLIPVNNKVGLHYRALASENRPFLLPSLFFYYDQLDFKYVEGFIG
                     QNIYDLKSDTIELDLYDGFVQGGLYIDEPRVTMQVVSSFGFPTKALVHTFEMRSGQGA
                     PVSFQSKILDEGMFFDYPALGQAGQFKTTTFVFDPSNSNIREVFNAQARFLIYDVDAL
                     SNPLLVPDSSYFITDSSRFTIHVSVDLPLRGRIRQYPASKIFNLDGGQLSELKEGRLV
                     VQTENGLPLSASAQIYLNDLSGMLIDSVFTGYKDLFKPAPTDSEGKAAGTASYQYEIP
                     VPAARIGAWTKTRSVRVEVFFDSPPGGQVVGIRAEDKLNIKLGFIGKIK"
     gene            complement(12744..14144)
                     /locus_tag="PKM27_15220"
     CDS             complement(12744..14144)
                     /locus_tag="PKM27_15220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR08669.1"
                     /translation="MKIRFCGATKEVTGSCHLVILEDGFKILLDCGLFQGDNDDELNR
                     IWLFKPGEIGAVVLSHAHIDHCGRLPKLAKDGFEGNIYCTHATRDLSAIMLMDSAKIQ
                     ENDAEYENKKNTKSGKKQVQPLYTLKDAGRVMKNFVSFNYNRWFRIHETVEVLFKDAG
                     HILGAASVTLRIREGKRMVMLGFTADIGRPDRPILKNPEPMLPVDFLICESTYGDRLH
                     EEGPAEEQHFLRIIHETCVKKKGKLIIPAFSVGRTQEIVYMLDQAENKKILPKVKVYV
                     DSPLAVNATNVFLEHPECFDQELHEYLLFDKNPFGFNGLHYIKSVEESKALNGSDEPC
                     IIISASGMANAGRVRHHLANNIEDPKNTILIVGYCTPNTPGGMLRNGIPEIKFFGESK
                     KVNARVELMDSFSAHGDQKEMLQFIRNQLPSVERTFLVHGDDRVLPVWRSVLQENGFQ
                     QVTVPSLGQELDITQP"
     gene            14216..15331
                     /locus_tag="PKM27_15225"
     CDS             14216..15331
                     /locus_tag="PKM27_15225"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR08670.1"
                     /translation="MESKIIKYSLYALVFSLLAFNCKKRESPLPGLVRGYEVIDSRQS
                     PGYVLQVMKGDSVLLEQMEGVEDLATRKPISAKSLFNTGSISKTFVAYAILHLAHEGR
                     IHLNDSLLKYFPDFKNQGIGKKVTLYHLLTHSSGLPDNRPVQKDSLFYLTADDAQNWA
                     PLLQNDTLHFEPGTKYEYSNPAFNALALVIQQVTGIKWQDYIKLKIFTPAGMATSTIT
                     EGMHPEEGVCHAYLPLADGQWKEQDYGEEPTFNASGNGGVWSSVQELAFYEKAIRLGL
                     FFEPEVLNLAREIYPLPGWRDSIPSQLGLSWFIGTFAGHPMYSHTGSQGGFTADFVSI
                     PEEDFFYTILSNTPLDILETRAYVLHLALERGWLNPK"
     gene            15412..16890
                     /locus_tag="PKM27_15230"
     CDS             15412..16890
                     /locus_tag="PKM27_15230"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08671.1"
                     /translation="MKYWLVYLLFLPVWAPGQQYSIKVNGGYGSGLFNAGDTIHIWAR
                     EEKLTETFQNWSGDTSWMINPEEWHAIGIMPPRNVTVTAEFQDLPSGAKLTFERMQGR
                     DTLKPVQYYFPPKGPTAKALVWIFHGTGGSFHSITDQIEGTSIVKKLVANGYAVAISE
                     SEESTYRRDFDGDGNLRWRYVASADNVDMQNIQQFRDTFVARGYIDPLLPNMGLGFSA
                     GGAFALINGWFMKFARGVSYCAPGSVGIADKSDMPSQWFMGFNDNHPDVGKAGNDTAR
                     IISAALDQRGVCSQFNLFPPSPCYPEYFRRISGIDPVLSAQIFNELMTMGAFTSRNFP
                     KLYPDQIKGIVLTSPSKYPALSSLTAAQIIHLDDQLIVLIAGHHVQSHFNGRMLRFLD
                     EACGEITTPYSDLRSPLGFELWPNPASHRLFIRLVGVQDQKISVQLTGLDGKLIRYYT
                     RDHHQKESIELNLPESLRSGVYGLRLTGSLGYRNSEKLTILQ"
     gene            complement(16941..>17518)
                     /locus_tag="PKM27_15235"
     CDS             complement(16941..>17518)
                     /locus_tag="PKM27_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08672.1"
                     /translation="YVNLYVKNEHHDGYYRDSCVFRNEIDIWDKMSAQIIYANGVTVN
                     YSLTTYSPYEGWKIAFNGFNGRIDSWEDIPFQKADVDQANRHAAEMTQTRNEKPESFD
                     EIFVMDNFSKAGETLYVPKYRGGHGGGDSRMQDLIFNNPKNENPYKIMAGTRDGAMSC
                     LIGIAARKSIALKRPVRIDELTDLKPSSNRM"
BASE COUNT         4151 a         4662 c         4303 g         4402 t
ORIGIN      
        1 attggccgct tcgaccgcct gctgaacatt catgccgaat tccgtcatat taaggaaaaa
       61 ctgcagcagg ttctggtcct gggaatcccc tccctgcacg ccaaaagcca ggagtggctt
      121 tccatcctta agtgcaaggg ttggcgtgag ggtcacccgc ggcctctgtc ccggcgcgac
      181 aacgttaaat ggattttcgc tggcatccaa aacaaaggat tgcatccgct ggctgaggcc
      241 gatcccggta ttgccggcaa tgcaggcggg catccagccc ccgcttggga ctacgctcac
      301 cacccagccc tgggcgtcgg cagccgtgac ggaggtcgtc ccggcataaa agttcttcat
      361 atactcctcg agatcggaag gccggtgtga acttggtttt ttcattcttt ctataaggtc
      421 caggtatgga ttggttttgc cttcaaaagg ataaggatcc ccgggcaggg ccatggcgtc
      481 gtttttggca gtatcgatca gcttgagcct ttgggcggca taagctttgg ataaaaggcc
      541 ctggatcggc tcgaccgggg gcacataagg atccccataa taaaaatcgc ggtcggcata
      601 ggcataattc atgacctggt agagggtatg aatgtattgc agggaattat aacgcatttt
      661 tttgaggtcc aggttttcga gcatattgag cgcctggagt aaaaccgggc cctgctgcca
      721 ttccctcagc ttgtacactt caatcccctt gtaattggtg gacagcggtt tctcgatcag
      781 gaccttccat ttggccaggt cttccttcgt gatcagcccc ccctgttccc gggtgccgcg
      841 cacaaattca tccgcgatat ccccggtata aaaacggtca taagcagcca tgatggcttc
      901 tttgcgactc ttgccggacg ccagcgcggc cgcttcggta tccaccattt ttttaagagt
      961 gacgtacagg tcgggttggg aaaacaattc accgggctgg ggcaactggt cccccttgaa
     1021 aaaaactttc tttgaataag gccaggattt gaggtcatcg gcataccgtt tcatctggcg
     1081 gtgtacatcc tcgtccatcg ggtatccatc ggccatttgc atggccggag ccaatacttc
     1141 ccggagactt tttgtgccaa attctgccag catcaccagg atcccgccgg gggttccggg
     1201 cgtcacggcg gccagcgggc cggtggccgg gggatatttg aatcctttac ttttataaaa
     1261 ttgagcagtg gcccccgtgg gtgctatacc gagggcattg atgccgacca cttctcctgt
     1321 tttcgggtta tagatcaaag cttgcgtttc tcctccccag ctcaatacat caaacatggt
     1381 gcaggtggcg gcaatcatgg cgcaggccgc gtctaccgca tttcctccct gttgaaacaa
     1441 agtagcccct gccgtcgccg caagcggttt tccggtaatg gccagccaat gcctggcatg
     1501 aagcactggt ttctgggtcc tgagggattg cacattggcc tgggccggca agtttgtact
     1561 gatgaccagg caaaggaaaa aaggaagaat ggtcgttttc atatttaaat ttagggataa
     1621 ccgctgaacc ggtgaaagaa atccactggc tttgcatgcc tgtcgcgcca ttctgcaaat
     1681 gcagtatctt tgaaaacatg aacacaaaat gcattgaatg cggggataaa atcatgggcc
     1741 gttcggataa aaagttctgt acagaccaat gccggagcgc atacaataac cgcctgaaca
     1801 gtgacagcaa caacatcatg cgcaacatca acaatacctt gcgcaaaaac cgcaggatcc
     1861 tcctggaagc caatcccgag gggaagggca aaatttccag ggaacagctg gtcaaacggg
     1921 gatttaagtt tgattacttc accaaccttt accagaccaa aaacggacat acctattatt
     1981 tctgttatga ccagggttac cttcccctgg atcgcgactg gtatgccctg gtcaaacgcc
     2041 aggaatacat tcattgaatc tgatcgttat taaaagaaat atgcgcagcc ggacttcctt
     2101 cctttccatt ttattattac tcgtgtccct ggctatacat gcccagaccc cgaactggtc
     2161 ggagacggtg gcaaagatcg tatataccca ctgcacgccc tgtcaccgga gtggcggttc
     2221 ggggcccttt tccctcatgt cctatggcca ggctttcaac aaccggttta gcatgcgttc
     2281 ggccgtgagc cagaaaatca tgcccccctg gcctccggat gattcgtaca gcgccctggc
     2341 ccacagcagg gccctgaagg aagcagacct cgaagccatc gttcgctggg tggatggcgg
     2401 agctccttca ggtaatttat ccacggctcc gcaagccccc caatatgaca acctgggata
     2461 cattaaaaat ccacagttgg tgatctccac ccccgaatat accctggcgg ggaataccga
     2521 tgaataccgc tgttaccccg aaagcagcaa tataaaccag gagatgtggc tgactgccct
     2581 ggaatgcatc ccgggcaatc cggggatcgt gcaccatatt ttggttttca gggatggcgg
     2641 gaaccggagt tttcagctgg accagcagca ggcaggcccc ggctatctct gttttggcgg
     2701 cgcggggacc tccggggcgg aactgatcgg ggcctgggtg cccggggccc aacccttcgt
     2761 cctgcccaaa ggatttggca tccggctgcc tgccaacagc aatatcatca ttcaggttca
     2821 ttatcccggc gggtccaagg ggaagtccga caagaccatt gtcaaattcc agctgaccga
     2881 tcagccccaa agggatgctt acctggttcc tgccctcaat cattccactt caatgaccaa
     2941 cgggcccctg tatatccctg ccaacaccaa aaaaaccttt aaccagcaat ttgccttgcc
     3001 gttcaacgct tcggtcattg gcgtggctcc gcatatgcac ctgatcggca cgtccatccg
     3061 gacctgggcg gttgggcctg atcagaaaga aatcccgatg atccatattc cgcgctggga
     3121 ttttcactgg cagggcattt accaatttca gaaagtcatg aaggtgcctg caggttcgat
     3181 gatttattcc gaagcgacct atgacaatac gccggccaac catcacaatc ccaatttccc
     3241 gccaaaagat gtgtcggtgg gggaagcaac cacggatgaa atgatgatca cttattttct
     3301 gtttaccctt taccaaaacg gggacgaaaa aatcatcata gattccgcga aagccctggt
     3361 ctcttcggcc ccggatgcgg agatcgaaac caggcccgtc acggtctttc ccaatcctgc
     3421 cggcaggtta agcccttcgc gattggtatt taaaacagat ccaaacgatc gcattgaact
     3481 cagcatcctg gacctgaccg gcaaggaaat cttccggaaa aaatacctgt actccgctgc
     3541 cggcacccag accatcggcc tgggagcaga ccggttggat accggcactt atatcatccg
     3601 ggtccgggga cagaaatggc aggccacaaa aaagtggatc ctgacgcctg aatagtttac
     3661 cggaggtctt cgctccgggg ccggcactat gggccttgcc aaatttgcag tcctcgtatt
     3721 tgccataaaa gaatatttta cagccggaaa aaatgacctc atcttggata gcgtggagac
     3781 cggcataaaa aagaaatttt atgacaaggc ttactggtac tttatgctgg ccgccctggt
     3841 caccattatc ggttttttcc cttcttattt cagccggctg accgtcaccg atcccgcaca
     3901 ccacctgcat ggcatcaccg ctacctgctg gatgctgttg cttgtcgtcc agccctggct
     3961 gtacaaaaca ggaaaattat ccttgcaccg aaaccttggt aaatcttcct atgtcctggt
     4021 acccctgatc ctgctcagcg gattgaatat ggtgcatatc atgctcaata accaggtcaa
     4081 ctatccccct gacctgcctt atcggctggc attcattgat tttatcaccc tggcccagtt
     4141 tcttttattt tatggcctgg ccatcaacca ccgaaaaaaa ttacaattgc acgcccgtta
     4201 catggcctgt accgtattgg ggcctctgat ccccgccctt acccgggctc ttttcctgat
     4261 cccctggatt gatcatttcg ataaagcgct gaacaccagt tatatcctca ttgaatcggt
     4321 attggtgatt cttttgctgg acgacaggcg taccggcagg atacgattgc cctatgtcct
     4381 cgcgttgatc ctgtttttcg tgcaacatct ggtcatgaat tttgtagggc aatggacctg
     4441 gtggcggtcc ctgatggacg cttacgccgg attgaggttc tgaatagtat tggaaaacaa
     4501 cagaataccg gccgggaatt gtaccgggtt attatctgcg ttcatggttt gtttaattta
     4561 gtcgggtgaa agcagatctg gaagaactgg taagcaataa ggtaatagac ccggaaacgg
     4621 cccgccggat ccgggaatat tacgacctga aatcaaaagc atcgaaagca tcccggctgc
     4681 tcctgatctt gtccatcacc ggcattgtgt tggttgggct cggactgatc ctcctcatcg
     4741 cttacaattg ggaatccctt ccaaaagcat tcaagctggc cttcgccttt ttaccccttg
     4801 cagccaccca gacctttggt ttatgggtcc tggtaaaaaa agggggtttg gtcctgtggc
     4861 atgaatccgc agcccttgcc attctttttg ccgtcgggat cgccatgtcc ctggtcaccc
     4921 agatctatca tatcgaggga gccctgtccg gctttcttca atggtggatc atcctttcgg
     4981 cgcccctcgt cccggttttt atatcggatg tggtttcttt ggctgtctgg atcgggaccg
     5041 gctggtacct gacttccatc tcctggacaa ggtccgatta tgatcttttt gctcccgcgg
     5101 tgatcctgtt ggcgctcggc tggtattcct ggcgcttgag gcatgaaggg ttaaaaaaat
     5161 ggatgacctt tcatcactgg gtcattccca tagtactgac catctatatt ttttcactgg
     5221 caccccatac ctgcaaccgg tttttactga tcgccctgtt gactttgttt atgggattcg
     5281 gtggggtcgc tttcacggtt tttaaaggac agggtctgct gaccaacggc ttccggaccg
     5341 gccaattcct gggtacctgg attaccggca tcattcttgg atttgaattt tactggactt
     5401 caccccttca ggattccctg tccaggtgta ttgctgataa ccctgttttt ttaccactgg
     5461 ttaatctggc agtcctgggt taccttgcct atgccttgtt tcgaaggttc aaggaatccg
     5521 agccggatga tctgggatgg atgggactgg ccctgatcgc ggtagtcatt gccggttcag
     5581 ggtaccctgg ctttgggcat atcgccgcca atattatttt attggccggg tccgcgagga
     5641 tcatctatgg cggtctttcc agggaaaatc tttggttact caacctgggc ctgcttaccc
     5701 tggccagttg ggtaatctcg cggttttttg aaaccaatat atcctttgtc tggaaaggct
     5761 tattgtttat caccctgggg atcctttgtt tcggcctgaa ttggttcatc cttaaaaaac
     5821 aaagaacatg aatacccccc taattaagat caccggcttt atactcctct gtctttttca
     5881 atggatcctg ccggtcagct ggatccttca ggaagaaaaa accaggaccg aagggatttt
     5941 tgtcaagatt ccctgccgtc cattcgaccc ggccgatccc tttcgcggaa cctttctgaa
     6001 tatacagccc ctgcctgcgg agtttgacta cgaagacagc agccgctttg aaaatcagca
     6061 ggaagtatgg gtcaactata ttcccgattc cggagaaatt taccggttcc attccgttca
     6121 gcccttgtcg gaggccccgg cacaaccggt tcacttcaaa gtcaccatcc ggaataccta
     6181 tgtgcagggg cccggcgccc agtacacagc cttcctggat tatccttttt ctaaatattt
     6241 tatcagcgaa aaagaagcgc cgcgacttgc tgaaaaatac aaccaggcgc tgtccgacac
     6301 cacccaaacc gtctatgccg gcctttttgt cctcgatgga aacgcttccc tggatgggat
     6361 ctggattggt gcagaaaagc tggagtgatc acaattccaa tctcttggcc gcagatttat
     6421 ttagttgcct cacctttcaa ttcaacaatt caacgacgcc tcccctttca ccaacccccg
     6481 atttccgctc tctccctgta aaattcaata ccctcgttga tgatgcggaa tgccgtttcc
     6541 ctgccaagga agctggttgc tgttactttt ttattttcaa gtaccgaata agtattgaaa
     6601 aactggatga tttctttttc caggttcggc ggcatgtcgg caatgtcgca gatataattc
     6661 acgctggggt cacgggcagc tacggccagg atcttatcat ccgattcccc ctggtcaatc
     6721 atttgcatga cccctataat attgacttct accaggcaat gcggggtgat gtcaatttgg
     6781 gacaatacca acacatcgag cgggtcgccg tcggcatgca tggtcctggg aataaagccg
     6841 taattggccg gatacatgac agaagaatac aggacccggt ccagtttcag cagaccgctt
     6901 tttttatcga tctcgtattt ggcgcggctt tttctggaaa tttcaataaa agcccagcag
     6961 acttcaggcg cccggtcgcc gggatccact tcgtgccagg gatggagaat cataatttaa
     7021 ttaaggaatt aaggaattaa gaatgaaagt tagaccggac acttgaggtt accttctggc
     7081 ccagagatgt ttccatgggc taaaggtaga aaattagatt gagcgggaga ccccgctgtg
     7141 ggcttttagg gatcctacga agcccttccc ttttgtcctg ctcaacgctt caataaatgc
     7201 ttcagcgccg aatccagatc cggatacctg aattcaaatc cttcctcaag cagttttgcg
     7261 ggcatcacgg ccagggactc aaacaaggta tcggccaatt ggcccagggc cagtttcagg
     7321 ccccaacgcg ggaccggcac cttcaggatg aatggacctg caagcctggt gatccgggtg
     7381 acaaattctt tttggctgac cggggcggga gcggtgacat gataaaccgg tgcggtgtct
     7441 tcccgggtca acattctgca aaatattctg accaggtctt cgatatgaat ccaggaataa
     7501 atctgttcac ccttgccaaa aaccggggca atacccaggc tggccggtag ataaaacttc
     7561 ttaaggatgc cttccccccg gctcaggacc gggctggtcc ggaggatcac tttccgcact
     7621 tcgggatcga ggccggacac gaccccctcc caatccctcg acagatcaga aataaaggaa
     7681 gagggccccg gcggagcgct ttcatccagc aattccccgg gccggtcgcc aaagatcccg
     7741 atgcccgcca tagaaagata gaccttgggg ggacgcacct gttgattgat acactttatc
     7801 aggaagcggc ctgaattcac cctgctgtca tataatactt ttttccggct ggcggtccat
     7861 ctcttttttt ctatggattc tccggcaagc tggacgacgc catagacccc atccaaagct
     7921 ccgggttcga gatattcccg ggccggatcc cattcaaact gggcaaagga tctgcccggc
     7981 ctttttgtcc gtgtaatgat gcggacctgg tagcccatgg aagtcaaata gaccgacatc
     8041 gcctttccga tgaatccgct ccctccacaa accgcaatga ttttattcat cctccaagat
     8101 tattggaata aaatactact tttaattgat ccttgaccca aattactgtt tctatgcctt
     8161 tgcctcctaa aaatgcgtat atccctttct tgttattgtt gagtctcctg gcttgtaaca
     8221 aagcaaggac cccgctccag gagggagttc tccaggtgcg cctggcgcgg gaaccagata
     8281 tcctcaaccc cgtactatcc gccacttcga ccgcggctgc tatagaaaac cttttatacc
     8341 tgcccctgat ggattttgac ccgtataccc aatcgttcaa acccgtgctt acttccggtc
     8401 ctgctgaaat catccgtcag gaggaccgtg tcggctataa aataaatatc cgccaacagg
     8461 ccatatggga cgacggcagt ccggtgaccg ccgccgacgt gagtttcacg ctgaaagcca
     8521 ccctaaaccc tttcgtccag tcggtgggaa aaagaagtgt gatttatgct attgacagca
     8581 tcgaattctc cggggacccc aggcaactgg tcttctggac cgacgaccgt tatttcctgg
     8641 atgaatattc tttcaccaat aattttatcc tgcaggccgc ccgcctggac agctcgggtt
     8701 tgcttgaaga cctcagttgg agcgaactga aatacatgag tccggaggat accggtgccg
     8761 cagcagccag ggcgagggat ttcgcggcat ccttatccag gccggaattc ggccgggaag
     8821 gtttgtcggg cggaggacct taccgcctgg aacagtgggt ggcgaatcaa tacatcaaac
     8881 tcaaaaggaa agaccaatgg tggggacaag cgtttgcaga ttccgcggtc aatttcaggg
     8941 cctatccctc agaaattact taccgcatta ttcctgaaga agcgaatgcc atcacggcgc
     9001 tcaaggaagg cctcctcgat gtgctggccg ccgatatcag cccatccaat ttcaaaagcc
     9061 tgagccagga cagctccctc caaaccaaaa taaattttat tgcaggtcct cccatccggt
     9121 tcgtttacct gtccctgaac aaccgcaatc cactgctgga tcaatccgaa acccggaaag
     9181 cgctcgcgcg cctgcttgac cttgaccagt tacaaaataa tttattccag ggttatgccc
     9241 agcggatcga agcccctttt caaccggcca aggaacatta tcaccaccaa ttggcgccgg
     9301 tcccctttga tatcgaaggt gcaaaatccc tgctttccca ggccggatgg acagatagca
     9361 accgcaacgg cacggtggat aaaaaaatca acaaccggca gacggaactc aagttgcgca
     9421 tgctggtcac tccaggggga ttgagccagc aggtggccat tcaccttcag gagcaaggac
     9481 gcaaagcggg agtggatatc gaaatcattc ccaaaccgat caatctgttg ttggaaaacc
     9541 tgcgtaacca tgactttgaa attgcggcac tggctgacat tcaatatccg ggtccggacg
     9601 acccttaccc tttctggcac agtgagagtt atatttccgg cggccaaaac tatacgggtt
     9661 tcggctcgcc ctttaccgac agcattataa acgaaatccg gtttggaagt tccgctccga
     9721 agcgaattgc gctctacaag gaactgcaag aggaaattta tgaccagcaa gccgccatat
     9781 ttttattcgc cccgcaaaac ctcatagccg taaataaaaa atgggatgcc cgggccgcct
     9841 ccgtaagacc aggatatttc atcaatgatt tcaggctgca tgcccgatga ccgaaccagc
     9901 ggtttggcat tattcagaac gatcggttaa ctgcttttca atcccacatc aaagccaagc
     9961 cgaacctggc ttgtaaccgg tcggccttat tcaatttcca cagggccgga acgtgatcgg
    10021 tggcgaaaaa caggttgaac acccccaact ccagggaggt cagaagaccc aggttccagt
    10081 ccttgcgggt atccaggctc atctgccctc caaagctcca gataggaaat ggcctgtaca
    10141 cggcgccagc catcaggtat ccataatccc tgctaaaaat atagtccgcc gaaaagccta
    10201 ctgtccaccg gtccgaaaaa taaacctggt tttccgccag gagactggcc ggtaaggacc
    10261 tggtaaaact ggcttcggat ttttcaacac ccgccagggc atggagggta tcccggacct
    10321 gatcaaaaat attttcattc aggttcaaaa aatcaatccc ctgaattccc tgatacaagg
    10381 tattgccgga acgtgaaaac cggtttcctt ttctccaccg gatgcttccc aggtctttga
    10441 gctggagcgc ggccctccat cggggggctt tatattgaac cccgatagaa aatgcaaatc
    10501 cgggatgccg gcctggtgcc agcccattcg acttactcaa acggatgctg tccaccgaaa
    10561 acgtctccac aaggtctgcg gagtagaagc tccagtcttc ctccattttg aggacgaggg
    10621 gatcccggat atccgctttt agtttgagga tatcggtatg gaaatcctgg atgcccccaa
    10681 ggtacctgac ggcggctccc accgaccatt tgttcctgaa aagggcggat tgcaaggtga
    10741 atgcctggaa aatgaaatgt tctccctcag actttaggtc caggggctgg gaggaggctc
    10801 gcggttccac ctgcaggtac ccatagttgc cgaagccaag caaacctacc agatctttgt
    10861 taaaacggag ggacgaatgg ccttcccatt catgactcag tcccagttgg ccgtatttcc
    10921 attttttacc taggaaaaag gtcctgacct tccattctcc atcaataaaa taattatttt
    10981 tttccaggcc cgcccacccc gaggcaagag aaaagtcttt gcccccgtcc gcccggtcaa
    11041 tcagccaggg gccttttccg gtttcgagga ccaggctgcc ggctccttcg ggcaggccaa
    11101 tcacgaattc tttaggaaaa ggaagcgccg ggttgcggcc ggtgtaaatc tgtgtattgt
    11161 tgaacaggga gatgccccgg tcctgggctg acagggcagt taccgcacac atcaaggttg
    11221 gggtcaatat gcgcctcagg gtatgcttca ttttattttt ccgatgaaac ccaattttat
    11281 attcagttta tcttctgcgc gaatgccgac cacctgtcct cctgggggag aatcaaaaaa
    11341 tacttccacc ctcacagatc ttgttttagt ccaggccccg atcctggcag caggaacagg
    11401 gatttcatat tgatagctgg ccgttcccgc agccttacct tcgctgtccg taggagcggg
    11461 tttaaacagg tctttgtatc cggtgaatac ggaatcaatg agcatcccgc taaggtcatt
    11521 caaatagatc tgggccgagg cggacagggg cagtccgttt tcggtctgga cgaccagacg
    11581 gccttctttc agttctgaaa gttggcctcc atcgaggtta aaaatttttg aagccggata
    11641 ttgccggatc cggccccgca gcgggaggtc cacggagaca tggatggtaa aacggctgct
    11701 gtccgtaata aaataacttg aatccggtac cagcaatgga ttggacaagg catccacatc
    11761 gtagatcaaa aacctggcct gggcattaaa cacctccctg atatttgaat tggacggatc
    11821 gaatacgaag gtggttgttt tgaactggcc cgcctgtccg agggcagggt agtcgaagaa
    11881 cataccttca tccaggatct tgctttgaaa tgaaacaggt gcaccctggc ccgagcgcat
    11941 ctcaaaggta tggaccaacg ctttcgttgg aaaaccaaag gaactgacca cctgcatggt
    12001 gaccctgggt tcgtcaatat ataatccgcc ctgtacaaaa ccgtcgtata aatccagttc
    12061 gatcgtgtcg cttttcaggt cataaatatt ctggcctatg aagccttcga catatttaaa
    12121 gtcaagctgg tcataataaa aaaacaagga aggcagcagg aaggggcggt tttctgaggc
    12181 cagggcccgg taatgcagac ccactttgtt gttaaccgga atcaggcgaa tgtcttccag
    12241 gggatgcggg cccgagatgc ccgaagtatt ggtttctccg gcataaggga tttcaagttt
    12301 atgctgaacc gtctgaccct gataaaccat ttccggaatc catagctgaa ggcttaccgg
    12361 ctccgtttcc tggtgcccga aagcaaacga ataatttcct ttgccgagta cggcccttcg
    12421 aatgatcata ttgttgacca ccggaagggt gacctcatta agggtatcgg tcattggaat
    12481 gggcaggttg ccgggtaagg gttggaaaat atcctttttg gttttttgaa ggatctctcc
    12541 ggtatagtga atagccaggg tcccgtcctg actaacccgg atttcaccca gggtcactga
    12601 attttcaagc agggtcatgg ctttgatttc cccgttcaac aagggcaggg cccattcctc
    12661 ccggtcccgg tccagataat attccgggag attgcaggca aagcctgcgc acaacagcca
    12721 gactaagatc gtgcggatcc ccttcacggc tgtgtgatat ccagctcctg tcccaatgaa
    12781 ggcaccgtga cctgttgaaa tccgttttcc tggagcacac tgcgccaaac cggaagcacc
    12841 cggtcgtcac catgtacgag gaaggtcctt tcaacggaag ggagctgatt ccggataaat
    12901 tgaagcattt ccttctggtc cccgtgcgcg gaaaaactgt ccatcagttc gaccctggca
    12961 ttcacctttt tggattcacc aaaaaatttg atctccggga tgccgttcct caacatgcct
    13021 cccggggtat tgggcgtgca gtacccgacg atgagtatgg tgtttttcgg gtcttcgata
    13081 ttattggcca ggtggtgcct tacccgcccg gcattggcca tgccggaggc gctgatgatg
    13141 atgcagggtt catcggagcc attgagggct ttggattctt cgacgctttt gatataatgg
    13201 agcccgttga acccaaatgg atttttatca aacagcaggt attcatgcag ttcctggtca
    13261 aaacattcag gatgttccag gaagacgttg gtggcgttga cggccagggg gctgtccaca
    13321 tatactttca ctttaggcag tattttttta ttttccgcct ggtccagcat gtacacgatt
    13381 tcctgggtac ggccgacact gaaggccgga atgatgagtt tgcccttctt ttttacacag
    13441 gtctcgtgaa tgatccggag aaaatgttgt tcttccgcag ggccctcttc atgcagccgg
    13501 tctccgtagg tcgattcaca gatgaggaaa tcgaccggaa gcatgggctc cgggtttttc
    13561 aggatgggcc ggtccggcct tccgatgtcc gcagtaaatc caagcattac cattcttttc
    13621 ccttcccgga tgcgcagggt cacgctggcc gcgcccagga tatgcccggc atctttgaac
    13681 aatacctcta cggtttcatg gatccggaac caccggttgt aattgaagct tacaaaattt
    13741 ttcatcaccc tgcccgcgtc cttcagggta tacaggggct gcacctgttt tttaccgctc
    13801 tttgtatttt ttttattttc atactccgcg tcgttctcct ggatctttgc gctgtccatc
    13861 agcatgatgg ccgagaggtc ccgggtggcg tgcgtgcaat agatattgcc ttcaaatcca
    13921 tcctttgcca gtttgggcaa cctgccgcaa tgatcaatat gggcatgcga taagaccacg
    13981 gctccgatct ccccgggctt aaaaagccag atccggttca gttcatcgtc gttatctccc
    14041 tggaacaaac cgcaatccag taatattttg aacccgtcct ccaaaatcac caggtgacag
    14101 gaccccgtca cttccttggt cgcaccacaa aaccgtatct tcataaaccg tctttcttta
    14161 atgcgataaa gatagtttat attgtctgcc ggcacaaaaa ccgcttttat ttgaaatgga
    14221 atccaagatt ataaaatact ctttgtacgc ccttgtcttc agcctcttgg cattcaattg
    14281 taaaaaaaga gagtctccgc tgcctggcct cgtcaggggt tatgaggtca tcgacagccg
    14341 tcaatccccc gggtacgtcc tgcaggtgat gaaaggggat tccgtgttgc tcgaacaaat
    14401 ggagggcgtc gaggacctgg ctacccggaa acccatctcg gcgaaaagcc tttttaacac
    14461 cggatccatt tcaaaaacat ttgtggccta tgcgatcctg cacctggccc atgaaggcag
    14521 gatccatctg aacgacagcc tgctgaaata tttcccggat tttaaaaatc aagggattgg
    14581 gaaaaaagtt acgttatacc acctgctcac ccattcttcg ggccttccgg acaaccggcc
    14641 ggttcagaaa gacagcttgt tttacctcac cgctgacgat gcacaaaact gggcccccct
    14701 tcttcaaaac gatacccttc attttgaacc gggcactaaa tatgaatatt caaatcccgc
    14761 ctttaatgcc ctcgccctgg tcatccaaca ggtgaccggc attaaatggc aggactacat
    14821 caaactcaaa atcttcactc ctgcaggaat ggccaccagc accattacgg aagggatgca
    14881 tcccgaggaa ggagtatgcc atgcctatct ccccctcgcg gatggtcaat ggaaggaaca
    14941 ggattatggg gaagaaccta cgttcaatgc ttcgggcaac ggaggggttt ggagcagtgt
    15001 tcaagaactg gcattctatg aaaaagcgat tcgcctggga ctgttttttg aaccggaagt
    15061 ccttaatctg gcaagggaga tctacccgct cccgggctgg cgggacagca tcccatccca
    15121 acttggtctc tcctggttca tcggaacctt cgcgggacat cccatgtatt cgcataccgg
    15181 atcacagggt ggatttacgg cggattttgt aagcatcccg gaagaggatt ttttttacac
    15241 cattttgtcc aataccccgt tggacatcct tgaaaccagg gcttatgtac tgcatctggc
    15301 ccttgaacgc ggctggctca atccaaagta aaaataaagg agactgaatc acccctacgg
    15361 gtgatcagat aatatatttc aggttctaat tttgaagaat tgattcaagg catgaaatat
    15421 tggttagtgt acctcctgtt tttgccggtt tgggctcctg gacaacaata ttccattaaa
    15481 gtgaacggag gttacggaag cggcttgttc aatgccggcg acacgatcca tatctgggca
    15541 agggaggaaa aactcacgga aacttttcaa aactggtccg gcgatacctc ctggatgata
    15601 aatccggaag agtggcatgc cataggcatc atgccgccgc ggaatgttac tgtgacggcg
    15661 gagttccagg atcttccttc cggcgcaaag ctgacttttg agaggatgca gggccgggat
    15721 acgctcaagc ccgtccagta ctattttcct cccaagggcc caactgctaa agcactggtc
    15781 tggatttttc acgggacagg tggcagcttt cattccatta cggatcagat cgaagggaca
    15841 tccatagtga aaaaactggt cgcaaacggc tatgcggtag caatcagtga aagcgaagag
    15901 tcgacttacc gccgggactt tgacggcgat gggaaccttc gatggaggta tgtagcctcc
    15961 gccgacaatg tggatatgca aaacatacaa cagttccggg acactttcgt agcccgcgga
    16021 tatatcgatc ccttgcttcc gaatatgggg cttggatttt cggcaggcgg tgcatttgct
    16081 ctgatcaacg gctggttcat gaaatttgcc cgcggagtca gttattgcgc tccgggatca
    16141 gtgggtattg ccgataaatc ggatatgcct tcccaatggt ttatgggttt caacgacaac
    16201 catccggacg tgggcaaagc cggcaatgat accgcccgga tcatttccgc agctctggac
    16261 cagagagggg tatgcagtca gttcaatctc ttcccgccct ccccctgtta tccggaatat
    16321 tttcggagga tttcgggtat agaccctgtc ctgtctgctc agatttttaa tgaattgatg
    16381 accatgggtg ccttcacgtc ccgtaacttc cccaaactgt atcccgacca aatcaagggg
    16441 atcgtgctta ccagtccatc taaatatccg gccctgtcct ccctgactgc tgcccagatc
    16501 atccacctgg acgatcaact cattgtgctt atagccggtc atcatgttca atcccatttc
    16561 aatggaagga tgttgcggtt tctggatgag gcttgcggag aaatcaccac tccctattcg
    16621 gatctcaggt cccccctggg gtttgaactc tggcccaatc ccgcctctca caggctcttc
    16681 atacgattgg tcggggtcca ggatcaaaaa atcagtgttc agttaaccgg actggatgga
    16741 aaattaattc gttactatac cagggatcac catcaaaagg aatccattga actcaacctt
    16801 ccggaaagct tgcggagtgg agtctatggg ctccggctta ccggcagcct tggataccga
    16861 aattcggaga aacttacaat attgcagtag cgtccgctct ttaattttta aacttaaaaa
    16921 acagacgcct tcaaaggacg ctacatccgg ttggacgagg gcttaaggtc ggtcagttca
    16981 tcgatgcgca cgggcctttt aagggcaatg cttttgcggg cggcaatacc gatgaggcag
    17041 gacatggctc cgtcccgggt ccctgccatg attttgtagg gattttcatt ttttggattg
    17101 ttgaagatca ggtcctgcat ccggctgtct ccgcctccgt ggccgcctcg gtattttggc
    17161 acatagaggg tctccccggc ttttgaaaaa ttgtccatga caaatatttc atcaaaggat
    17221 tctggctttt cattgcgggt ctgggtcatt tccgcggcat ggcggttggc ttggtccaca
    17281 tcggctttct ggaaaggaat atcctcccag ctgtcgatcc tgccattaaa tccgttgaaa
    17341 gcgattttcc aaccttcgta aggggaatag gtggtgaggg aataattgac cgtgacgccg
    17401 ttggcataaa tgatctgggc cgacatctta tcccagatgt caatttcatt tctgaacacg
    17461 cagctgtcgc ggtaataacc gtcgtggtgt tcgtttttta catacagatt gacgtatt
//
LOCUS       DAOQXT010000101        17489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3635_length_17489_cov_6.143341, whole genome shotgun sequence.
ACCESSION   DAOQXT010000101 DAOQXT010000000
VERSION     DAOQXT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17489
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3635_length_17489_cov_6.143341"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKM27_15240"
     CDS             <1..348
                     /locus_tag="PKM27_15240"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNR08673.1"
                     /translation="ELFESRGGKTAFFVSPNIMYTKQGNFSELLGGAYFGMGPLFAGL
                     WGRLTPTNPDAAILNIGLRRGNMKISYSFDATISKLSLGSGGAHELGIVFNLDDQSDR
                     KSPDYNDCFQLFR"
     gene            509..1960
                     /locus_tag="PKM27_15245"
     CDS             509..1960
                     /locus_tag="PKM27_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR08674.1"
                     /translation="MIAACSKKGGERSSSTGWKYNDQKWGGFEKLDYKGQVTGPNLVP
                     IEGGTFVMGLTEEDVPFQWNNEGRRVTVSSFYMDETEVSNIDYREYVYWLSRVYTSFP
                     MIAKKALPDSLVWREELAFNEPFVESYFRHPSYDDYPVVGVNWRQATDYCEWRTDRVN
                     EMLLIEKGILNPNPEQKDAENFNSKAYLTGQYAGNVRKNLKDLQSGGERSVRYEDGIL
                     LPSYRLPTEAEWEYAALALYGKQVSKNDELIAERRIYPWDGNTVRYKRRTKDQGKIMA
                     NFKRGRGDYMGTAGKLNDQGNIPTNVRSYVPNDFGLYNMAGNVSEWVADVYRPLTSTT
                     LRDEEEQDLNPFRGNEFRVIDLDSTGNVQKDSLGRIKYRTVQLDESLTRENYSRGNVI
                     DYRDGDDTTGAFYDYGVHTLISNHSRVVKGGSWADRAFWLSPGARRYKDEDKGDKTIG
                     FRCAMSRMGAQSMNDQKGLDFNVKPKAVKRRYN"
     gene            2028..3341
                     /gene="murF"
                     /locus_tag="PKM27_15250"
     CDS             2028..3341
                     /gene="murF"
                     /locus_tag="PKM27_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNR08675.1"
                     /translation="MDIRELYLEFLAGSGICTDSRLIKPAQLFFGLAGSRANGSDFAL
                     QALRDGARYAIVDDPALSGHPGCILVPSALETLQQLAGWHRQQLNIPVIGITGSVGKT
                     TTKSLIHAVLSSRYKAFCTLGNLNNHIGVPLSVLSIGSEHEIAVIEMGANHIGEIESL
                     CEIARPTHGLITRIGKAHLEGFGSLEGVLIAKTELFRYLRKNKGLAFVNDQDELVFAA
                     SKGFEMPKVMIHEITHSSILAADPFLTIRLELAGEPALEVVTRIPGSYNLENIEAAAC
                     VGKYFEVPLPGIKAALEKFESGSNRSQVIEHGGNSFLMDAYNANPVSMAESIRSFAAI
                     STQKKKVLILGEMRELGTDSPAEHQGILDLIAHFTWAGVILVGPEYKELVTREPYYHF
                     PDVASLKPWFQAQEFRDTFFLVKGSRGVALEQLFEPGTFNPGSAH"
     gene            complement(3319..4362)
                     /locus_tag="PKM27_15255"
     CDS             complement(3319..4362)
                     /locus_tag="PKM27_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08676.1"
                     /translation="MKEISNDTPFTAGSRNQGRIGHAVQAIKDPENQSGPSFALLGSN
                     DAADRIRAELFELADHFPLSNLIDLGNLRDGDPQEGPALFQSLFNAGIIPVFIASDPG
                     DFELHLKASLRHSMVSSWAAVHPSLLSDYPGRNSVFEAAESGNASFKFIGHQLHITPA
                     EQIEKTARLGLNSMRLGQVRQHIDAVEPWFRSLDHVLFDLDALRRTDFPSKKSINPGG
                     FFYEEACKICQFSGASSHLKSFGLYGYDPLSDTDGNGAAVAAQLIWYLFEGFNNHREE
                     KAIQKSKMTQYIVHNPQNGKDIHFWKSNQSGRWWLEIPDHEDHWISCTYDDYNAASKG
                     AYSSRIVSVLNLD"
     gene            4639..6288
                     /locus_tag="PKM27_15260"
     CDS             4639..6288
                     /locus_tag="PKM27_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR08677.1"
                     /translation="MTTDPEQNQKWRMGEYRYSAKPKNMWELGIHTGHYFIDGDVDPL
                     QPAGFGVGLHLRKAIHYVFSFRGDLTYAASKGLDPQLTPVDIAAQDLNATTAKGRHGN
                     IYRNFRNQDVHARLQGVLNIGNILFHKERNKWNWYVFAGLGLNYNKTKIDALKGNAAY
                     DFSSVSGDVDTKSGRKSIKKQVKNILDGVYETEARKKVAIFRFGDKTNIHAAFSAGTG
                     IARKISKRVNLALEHEVITSDNDLLDGFVYRSSVDQSNNNDVAHYTSLRLGINLGNFE
                     KKTEPLYWLNPLDGVLHDLTELKQRPILDLTDGDGDGVIDMVDQQKNSPMGAVVDTRG
                     VALDSDKDGIPDHLDKEPFSPPGFSTDAQGIAAIEADKGLTEDEVINLINQRISNIKT
                     DWFLPMIHFDLDKYYIKPEFYGQLHHVATVMKSHPDLKVAVVGHTDVRNPDDYNKVLA
                     FKRADAAMNYLISKYNLPRDRFLIQYGGEALPMVPGLPDSHNIDKQQEMSQYINRRVE
                     FRIATSTDQNMERPTGPDAGKNTPGSSRPGPKYSGNVNSGY"
     gene            complement(6395..6721)
                     /locus_tag="PKM27_15265"
     CDS             complement(6395..6721)
                     /locus_tag="PKM27_15265"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNR08678.1"
                     /translation="MASDQKFVDYITDQIRGAGDIVAKKMFGEYGIYSDGKIFGLICD
                     NKLFIKPTNSGRKFIGEAVEAPPYPGAKPSFLIGEKIEDHEWLSELVRISLKELPEPK
                     QKRKKS"
     gene            6894..6967
                     /locus_tag="PKM27_15270"
     tRNA            6894..6967
                     /locus_tag="PKM27_15270"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6928..6930,aa:Ala,seq:ggc)
     gene            complement(7302..10118)
                     /gene="carB"
                     /locus_tag="PKM27_15275"
     CDS             complement(7302..10118)
                     /gene="carB"
                     /locus_tag="PKM27_15275"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNR08679.1"
                     /translation="MPRDHTIKSVLIIGSGPIVIGQACEFDYSGSQASRSLREEGIEV
                     SLINSNPATIMTDPVTADHVYLWPLTVESLVKILEEQKIDAVLPTMGGQTALNLAVEA
                     EKQGIWAKYGVRMIGVDIDAIELAENREKFRNHVKQLGMSVPPSYIANSFLEGKNAAQ
                     KIGFPLVIRPSFTLGGTGGGFVMKEEEFDEALRRGLDASPSHEVLVEKAVLGWKEFEL
                     ELLRDLNDSVAIICTVENMDPMGIHTGDSITVAPAMTLSDTAFQKMRTDAITLMRSMG
                     NFAGGCNVQFALNPENEELIVVEINPRVSRSSALASKATGYPIAKIAAKLAIGYNLDE
                     LKNQITKTTSAYFEPTLDYVIVKMPRWDFEKFPGADHKLGLQMKSVGEVMAIGRSFLE
                     ALQKACQSQENRRMGLGADKKEWIQTQDILKRLETAFDDRVYRVKDALRLGVPEKTIH
                     KLTLIDPWFIKQIKSLVRIEDELMRYNVAEDIPAPFLREMKELGYSDGQIAWLLRIHE
                     REVYRHRMKLGIRRTYKMVDTCAAEFEARTPYFYSTFDRENESVPSDKKKIIVLGSGP
                     NRIGQGIEFDYCCVHGLIAIKEAGYESIMVNCNPETVSTDFDIADKLYFEPVYWEHLK
                     EILDLEKPEGVIVQLGGQTALKLAEDIHREGIRIIGTSFNSMDIAEDRGRFSDLLKEL
                     EIPYPEYGTARDADEALAVAKRTGYPVLVRPSYVLGGQKMRIVINDDELEHHVLTIFK
                     HMPENKVLIDQFLERAKEAEIDAIFDGEDFHIMGVMEHVEPAGIHSGDSHALLPPYSL
                     TEEEIRTMAFYAEKIARSLEICGLINIQFAIKDHQVYVIEANPRASRTTPFIAKAYQV
                     PYLNIATHVMMGTKKLRDFNILKNLQGYAIKVPVFSFEKFPHVDKQLGPEMKSTGEAI
                     YYIKDLKDPYFRKLVSEASMYLTR"
     gene            10254..11234
                     /locus_tag="PKM27_15280"
     CDS             10254..11234
                     /locus_tag="PKM27_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR08680.1"
                     /translation="MNPPRILITDEVHPVLLDGLREMAFDLDYHPAIAYEDVLQLIGS
                     YDGLVVNSKIICDQNFIDRAARLRFIARLGSGREIIDLEYAASRGIQTFFSPDGNNNA
                     VAEHALGMLLALANQLVKADRQVRQKIWEREARRGWELSGKTIGIIGFGYTGSQFARK
                     LAGMEMKVLVHDKYLSGFQSQLPFIVETGKEDIIREADIISFHLPLSAETYHYCNDDL
                     IGSCKPGVILINTSRGNVIDTKALVRGLTSGRVGGACLDVFENEKPMTFTAEEDRLYS
                     TLYAMDQVILSPHIAGWTRESKYKMASILLKKILNLYRSGDHENRSGATL"
     gene            11323..12720
                     /locus_tag="PKM27_15285"
     CDS             11323..12720
                     /locus_tag="PKM27_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNR08681.1"
                     /translation="MKNRYFDLIEQTFYFPQEGFDIENGYLDFNGIPLIHLIRKYGTP
                     LKLTYLPRIGAQIKRARNLFNRSMKNVGYNGQYYYCYCTKSCHFRFILEEVLQNGAHL
                     ETSSSFDIDLIRKLYMQNQLDKDEIIICNGYKAGDYGRKICKLINEGFKNVIPILDNK
                     NELGIYEEHVENGCKIGIRIATEEEPNFQFYTSRLGIRNSEVIQFYKERLANNPKFKL
                     EMLHFFVDTGIKDTIYYWGEFKKVLKIYCELKKICPELRAIDIGGGLPIRNSLGFEYD
                     YSYMINEIVKQIKIACQDEGIPEPDIFSEFGKYTVGESGATIFSVIGQKQQNDSELWY
                     MIDNSLMNTIPDSWGMAERFILLPINKWYNDFTRVNIGGITCDNSDFYNSEVHENQVY
                     LPAFSKTDPEPLYIGFFHTGAYQDALSGYGGIKHCLIPSPKHILVDRDEQGNLVDRVY
                     LDEQKADDMLKILGY"
     gene            12749..13723
                     /locus_tag="PKM27_15290"
     CDS             12749..13723
                     /locus_tag="PKM27_15290"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNR08682.1"
                     /translation="MSQGIISAFIEKHFRHFNAAALVDAAKGYQHHLDIGGKMMVTLA
                     GAMSTAELGKSLAEMIRQGKVDIITCTGANLEEDIFNLVAHTHYKRIPNYRDLTPEQE
                     QELLDHHYNRVTDTCIPEMEAMRRMEQQLIDVWNEAKKNGKRYFPHEFVYQIIRNKSL
                     EKYYEIDPANSWLIAACERNLPIIVPGWEDSTTGNMFAGHCLEQEYEPTMVRNGIEYM
                     MFLADWYLKNTEKIGVGFFQIGGGIAGDFPICVVPMIYQDMRRTDVKCWGYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLEVDTPKFIIESDATIVAPLIFAHILGW"
     gene            complement(13740..14750)
                     /gene="nadA"
                     /locus_tag="PKM27_15295"
     CDS             complement(13740..14750)
                     /gene="nadA"
                     /locus_tag="PKM27_15295"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNR08683.1"
                     /translation="MSLSSISAEQLQRDGYLHIPVSPDLDLEAEILKLKQEKNAVILS
                     HYYQESEIQDLADYVGDSLGLSQQAAKTKADIIVFAGVHFMAETAKILSPDKKVLLPD
                     LKAGCSLADSCPPDLFAKFKAKYPDHVVVSYINCTAEIKTLTDICCTSTNAIHVINSI
                     PPDKKIIFAPDINLGKYLIKQTGRDMVLWNGTCMVHEIFSHEKIVRLKERHPKAKFIA
                     HPECEPHILEMADYIGSTSGLLKYTMTNPAREFIVATEAGILHQMELKSPEKTFIPAP
                     PNNQCACNECPHMKRNTLEKLYTCMKYELPEIILPQWVIEQGRASIDRMLEISRRAGL
                     VG"
     gene            complement(14828..15541)
                     /locus_tag="PKM27_15300"
     CDS             complement(14828..15541)
                     /locus_tag="PKM27_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNR08684.1"
                     /translation="MGEPGILYLIPSPLDEEYQGVLIPEQVRVLEGLRFFVVENIRTS
                     RRFIKKILPGFDIDACYFLEMDKHQAGQDFSMLINVLNGGSSAGLLSEAGCPGIADPG
                     SQVVRLAHEQGIRVVPWIGPSSITLALMASGLNGQQFVFHGYLPTGRPDLIPRLKRVE
                     ADMVRTGYTQIFIETPYRNLSLLETLLANLAETTLLCLAIDITGPDEYIKTQTVKRWK
                     TAGLVHFGKKPAIFLIGAG"
     gene            complement(15552..16955)
                     /gene="lpdA"
                     /locus_tag="PKM27_15305"
     CDS             complement(15552..16955)
                     /gene="lpdA"
                     /locus_tag="PKM27_15305"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNR08685.1"
                     /translation="MDFQILIIGSGPGGYVAAIRCAQLGMKVALVEKYASLGGTCLNV
                     GCIPSKALLDSSEHYDQAMHQFASHGLRVKGIDLDMPQMIRRKDEVVDATVKGVKFLM
                     KKNKIEVFHGTASFKGPHEVEIKKSDGQLLTVQAEKIIIATGSKPLTPAAFHYDKERI
                     ITSTEALNIREVPGRMVIIGAGVIGLELGSVFSRLGTKVEVIEFLDRILPGMDGECAR
                     EMKKILEKKGMVFHLNHSVDGVIKSKKGVEVSYSDKNTKESFKLQSDYCLVAIGRRPF
                     TEGLDLAKAGVSQDEKGRILVNEQLQTAVPHIYAIGDVVRGPMLAHKASEEGILVAEI
                     MAGQKPHIDYNLIPGVVYTWPELAGVGKTEEQLKEEGNNYKTGKFPFKALGRAKASMD
                     TEGFVKVLSDASTDEVLGVHILGARAADLIMEAVSLMEFKASAEDIARISHPHPTFSE
                     AIKEAGFAAWTGKSLHL"
     gene            complement(16959..>17489)
                     /locus_tag="PKM27_15310"
     CDS             complement(16959..>17489)
                     /locus_tag="PKM27_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08686.1"
                     /translation="GIMYYELESTGKNKEAQVFGLRPEQVREIRYKNDRGEIPRDFGE
                     TVALTAPGGQADAEDAGFADVTGEIELPEEKRRKGRSRDHRNQGARNQNWQAGPPSDQ
                     NRPQRSRTDQTDRRGGQGRQGPRQDKPRQDQPRRERPNNPERPQNSSRPQDQSRKPRP
                     PFNKNKDRFNPPPSNS"
BASE COUNT         4204 a         4444 c         4544 g         4297 t
ORIGIN      
        1 gagctttttg aatcccgcgg agggaaaacc gcattttttg tgagccccaa catcatgtat
       61 accaaacaag gcaatttcag tgaattgctg ggcggcgcct attttggaat gggccctttg
      121 tttgcaggcc tctggggtag gctgaccccc accaatcccg atgccgccat cctcaatatc
      181 ggtctgagaa ggggcaatat gaagatttcc tacagttttg acgcgaccat ttctaaactc
      241 agccttgggt cgggaggtgc gcacgaactg ggtattgtat ttaacctgga tgaccagtcg
      301 gaccggaaat cgccggatta caatgactgt tttcagctgt tcagataaaa aatcggagct
      361 cttaagcaat aattaacacc aaacggccat ttattcctga gcattaggat tttaccgtat
      421 atttggcagt ctttttgaat accaaaattg caaccgttaa gacaataaaa aaatgagaag
      481 ggtttttctt ttagccataa cattttccat gatagccgcc tgctccaaaa aaggaggaga
      541 gcggtccagt tccaccgggt ggaaatacaa tgatcagaaa tggggaggat ttgagaagct
      601 ggattacaaa ggacaggtga ccgggccaaa cctggtgccc attgaaggag gcactttcgt
      661 catgggcctc accgaggaag atgtcccttt ccaatggaat aatgagggga gaagggttac
      721 ggtttcctcc ttctatatgg atgaaactga agtttccaat atcgattacc gggaatacgt
      781 ctactggctc agccgggttt atacgtcttt tcccatgatt gccaaaaaag ccctgcccga
      841 ttccctggtt tggcgggaag aactggcctt taacgaaccc tttgtagaaa gttatttcag
      901 gcacccttcc tacgatgatt atccggtagt gggggtcaac tggagacagg ccactgatta
      961 ttgcgaatgg aggacggacc gggtcaacga aatgctgctg atcgaaaaag ggatcctgaa
     1021 ccccaatccg gaacaaaagg acgctgaaaa cttcaattca aaagcctacc tgaccggtca
     1081 atatgccgga aatgtcagaa aaaacctcaa agacctgcaa agcggtggtg aacgaagcgt
     1141 aaggtatgaa gacgggatcc tgcttccctc ttaccgtttg ccgaccgagg ccgaatggga
     1201 atacgccgct ctcgctttat atggaaagca ggtttcaaaa aacgatgagt tgatcgccga
     1261 acgcagaatc tatccctggg atggcaatac ggtccggtac aaaagaagga ccaaagacca
     1321 gggcaagatc atggccaact tcaaaagagg aaggggtgat tatatgggta ccgccggcaa
     1381 actcaacgac cagggcaaca tcccgaccaa tgtgagatcc tatgtgccca atgatttcgg
     1441 cctttacaat atggccggga acgtcagcga atgggtggcc gatgtatacc gcccgttgac
     1501 cagtaccacc ctgcgcgacg aagaagagca ggatctcaat cctttccgcg gaaatgagtt
     1561 ccgggtcatt gacctggaca gcaccgggaa tgttcagaaa gacagcctgg gcaggatcaa
     1621 atacaggacc gtccagctcg atgaatcgtt aaccagggaa aactacagcc ggggcaatgt
     1681 catcgactac cgggacgggg atgataccac gggggcgttt tatgattatg gcgtgcatac
     1741 cctgatcagt aatcattcca gggttgtaaa aggaggatcc tgggccgacc gtgcgttttg
     1801 gctttctccc ggggcccgcc gctacaagga cgaagacaaa ggcgacaaga ccattggatt
     1861 ccgctgtgcc atgtcccgca tgggagctca gtccatgaat gatcagaaag ggcttgattt
     1921 taatgtaaaa cccaaagcgg taaaacgcag gtataactga tattcatata taataaaaat
     1981 tcataatata ttccctgtaa gcgctacctt gcagggaatt tttttttatg gatatccggg
     2041 aattatacct tgaattttta gccggaagcg gcatctgtac ggacagccgc ttgatcaaac
     2101 cggcgcaatt gtttttcggc cttgcgggtt ctagggccaa tggcagtgat tttgcactgc
     2161 aagcccttcg cgatggagcc cggtatgcca tcgtggatga cccggccctg tccggccacc
     2221 cgggttgcat cctggtgcct tccgccctgg aaacccttca gcaattggcc ggctggcacc
     2281 gtcagcaact gaacataccg gtaatcggca tcacgggttc cgtcggtaaa acgacgacca
     2341 agtccctgat ccacgctgtt ctgtcctcca ggtacaaggc cttttgcacc ctgggcaacc
     2401 tcaataacca tataggagta cccttgtccg tcctttccat tggcagcgaa catgaaatag
     2461 cagtcatcga aatgggcgcg aaccatatcg gggaaatcga atccctctgt gaaatcgccc
     2521 ggcccacgca tggcctgatc acccggatcg gtaaagccca tctggaaggt tttggcagtt
     2581 tggaaggggt tttaatcgcc aaaacggaat tgttcagata ccttcgaaaa aacaagggcc
     2641 tcgccttcgt caatgaccag gacgagcttg tctttgcagc cagtaagggg tttgaaatgc
     2701 caaaggtgat gatccacgag atcacacact cctcaatcct ggccgccgat ccttttctca
     2761 ccatccggct ggagctggcc ggcgaaccag ccctggaggt cgtcacccgc atcccgggat
     2821 cctataatct cgaaaacatc gaggcggcag cctgtgtagg aaagtatttc gaggttcctc
     2881 tcccggggat caaagctgcc ctggaaaaat ttgaaagcgg atcaaaccgg tcccaggtca
     2941 ttgagcacgg tggaaacagt ttcctgatgg atgcctataa tgccaatccg gtgagtatgg
     3001 cggagtccat acgctccttt gcggccatta gtacgcaaaa gaaaaaggtg ctgatcctgg
     3061 gtgaaatgcg ggaacttggg acggacagcc cggcggagca ccaggggatc ctggacctca
     3121 tcgcacattt tacctgggct ggagtcattc tggtgggacc ggaatataaa gaattggtaa
     3181 cccgggagcc ttattaccat tttcctgatg tggccagttt gaaaccctgg tttcaggcgc
     3241 aggaattcag ggatactttt ttcctggtca agggaagccg gggtgtggcg ctggaacaac
     3301 tgtttgagcc cgggacgttc aatccaggtt cagcacactg acaatccttg aggagtaagc
     3361 gcccttgctg gctgcgttgt aatcatcgta agtacaggaa atccaatggt cttcatgatc
     3421 cgggatctcc agccaccagc ggccgctttg attgcttttc caaaaatgga tgtctttgcc
     3481 gttctggggg ttgtgtacaa tgtattgggt catcttggat ttctggatgg ccttttcttc
     3541 gcggtggttg ttgaatcctt caaacaggta ccagatcaat tgggccgcca cagccgcgcc
     3601 attgccatcc gtatccgaca ggggatcgta accgtataag ccgaatgatt ttaggtggga
     3661 ggaggcgccg gaaaactggc agatcttgca ggcctcctca taaaagaatc cgccggggtt
     3721 tatggacttt ttggatggaa aatccgttct acgcagagcg tccaggtcaa aaagtacatg
     3781 atctaacgaa cgaaaccagg gttcaacggc atcaatatgt tgcctgacct ggcccagccg
     3841 catcgaattg agccccaggc gggcagtctt ttcgatctgt tctgccgggg tgatgtgaag
     3901 ctggtggccg atgaatttga aagaggcatt tccgctttcc gccgcttcaa aaacggagtt
     3961 ccggcccgga taatccgata gcagggaagg atggaccgcg gcccaggaac tgaccatgga
     4021 gtgcctgaga ctggccttca ggtggagttc gaaatccccg ggatccgaag cgatgaagac
     4081 cggaatgatc ccggcattaa aaagcgattg gaaaagggcg ggaccttcct gcggatcacc
     4141 atccctgaga ttacccaggt cgataaggtt ggaaaggggg aaatgatcgg ccagttcaaa
     4201 caactcagcc ctgatccggt ctgccgcgtc gtttgagcca agcagggcaa aggacggccc
     4261 ggactggttt tcggggtcct tgatcgcctg aacggcatga ccaatccggc cctggttacg
     4321 ggagcctgcg gtaaaggggg tatcgttgga gatttctttc atatctgact atgtgtgtga
     4381 ttttaataaa ttacaaatat atttaatgtg ttatataaac cgaaataatt tagctttttt
     4441 gttttttgtc gtatttttgg aaccgttcaa aacgttattc agagggctgc atttaactga
     4501 ataactgatt attacaaaat tttcgacaac atgcgttctg gttttttaag gatattggta
     4561 tttaatgctg ttttctttat agccgggtca ttgatatccc aggacgatac ctggactgca
     4621 cctaaaataa cggacaacat gaccaccgat ccggaacaaa atcaaaaatg gaggatgggt
     4681 gagtaccggt attcagccaa gccgaaaaac atgtgggagt tgggtattca taccggccat
     4741 tattttattg acggggatgt cgacccgctg caaccggccg gctttggggt cgggctccat
     4801 ctccgaaagg ccattcatta cgtattttct tttcgcgggg acctgactta tgcagcaagt
     4861 aaagggcttg acccgcagct gacccccgtt gatatagcgg cacaggacct gaacgccacc
     4921 acggcaaagg gaaggcatgg caatatttac aggaatttcc ggaaccagga tgtacatgcc
     4981 cgcctgcagg gggtgttgaa tatcggcaat atcctattcc acaaagagag gaataaatgg
     5041 aactggtatg tatttgccgg actcggactc aattataata aaaccaaaat cgatgccctc
     5101 aaagggaatg cggcctatga tttttcctcc gtcagcgggg atgtggatac caaatccggc
     5161 cggaaatcca taaaaaaaca ggtcaaaaat atcctcgacg gtgtctatga gacggaagcc
     5221 aggaaaaaag tggccatttt cagatttggc gacaaaacca atatccatgc ggcattttca
     5281 gcagggaccg ggatcgcccg aaaaatcagt aaaagggtca accttgccct ggaacacgag
     5341 gtgattacct cagacaatga cctgctggac ggttttgttt accgttcctc ggtggatcaa
     5401 tccaataata acgacgttgc gcattatact tcgctccggc tggggatcaa cctgggcaat
     5461 tttgaaaaga aaaccgagcc gctgtactgg ctcaatccgc tggatggagt attgcatgac
     5521 ctgaccgaac tcaaacaaag gcccatcctg gaccttacgg acggggacgg ggacggggtg
     5581 atcgatatgg ttgaccagca gaaaaactca cccatgggcg ccgtggtgga caccaggggg
     5641 gtggccctgg acagcgacaa ggacggaata ccggatcatt tggataagga accgttttcg
     5701 cctcccggtt tttcgaccga tgcgcagggt attgctgcca tagaggccga caagggactg
     5761 accgaagacg aagtcatcaa cctgatcaac cagaggatca gcaatattaa aaccgactgg
     5821 ttcctgccca tgatccattt tgacctggat aagtattata tcaaaccgga gttttacgga
     5881 caattgcacc atgtggccac cgtcatgaaa tcccacccgg acctcaaggt cgccgtagtg
     5941 ggccataccg atgtgcgcaa ccccgacgat tacaataaag tgctggcttt taaaagggcg
     6001 gacgcggcca tgaattacct gatttccaaa tacaacctgc ccagggaccg tttccttatt
     6061 cagtatggag gagaagccct gcccatggta cccggcttgc ccgactcgca taacatcgac
     6121 aagcagcagg aaatgagcca atacatcaac cgcagggtag agtttcgcat cgccacttca
     6181 acggaccaaa acatggaaag acccacagga cccgatgccg gcaaaaacac ccccggttct
     6241 tcccggccgg gcccgaagta cagcgggaat gtgaattcag gttattgatc accgggttca
     6301 cccgggttta agtggaatcg tggttgattc cggtcatgca ttaataaagt ttggtctttt
     6361 gactactgct gaatctgaat ccggttcgct gtagtcagga ttttttcctt ttttgttttg
     6421 gttcaggcaa ttcttttaat gaaatccgga ccaattcgct cagccattcg tggtcttcga
     6481 ttttttctcc tatcaggaaa ctgggttttg ctcccgggta aggaggagcc tccacggcct
     6541 cgcctataaa ctttcttccg gaatttgtgg gtttaatgaa cagcttgttg tcacaaatca
     6601 gtccaaaaat ttttccatcc gagtatattc cgtattctcc aaacattttt tttgcgacaa
     6661 tgtcgcccgc gccccggatt tggtcagtta tatagtctac aaatttctgg tcggaagcca
     6721 tacttctgtt tttatattaa agtacggatt taagattgtt ccggaagggt gttcccggat
     6781 attttcgtcc tgaaccctcc atgaaaagga tcagccatcg ttgaacctga gctcaataaa
     6841 actgatttca gaattctgaa aacattatat atttgcacct cgatttgaat tagggggcat
     6901 tagctcagct ggctagagcg cttgcatggc atgcaagagg tcatcggttc gactccgata
     6961 tgctccacta acacactatt tttgttactt atattattta tagtccaaaa ccggaccaat
     7021 attacataga ccacccttcc gatcaggaga aagattgact gaacaaaatg ggggcatttc
     7081 agttttcaca tctaaagtta ccgattggat cctgaaatat tccaggtccg atggtcttaa
     7141 atttgaaaag ggatatcaaa agcaaaaaaa gtcgaaaata tgtttttccc ttcatgaggg
     7201 ttggctagct tgctgctccg aaagctttag gaaaaaaggt cattgttacg attccatcag
     7261 caagacacca ttcaccaatc accacccacc atccaccatt cctaccttgt cagatacatg
     7321 ctcgcctcac tcaccagttt cctgaaatag ggatccttca ggtctttgat ataatagatg
     7381 gcttccccgg tgcttttcat ttccggccca agttgtttgt ccacatgggg gaatttctcg
     7441 aatgaaaaca ccggaacctt gatcgcgtat ccctgcaggt tcttgagaat attaaaatcc
     7501 cgcagttttt tggtgcccat cattacatgg gtcgcgatat tgaggtaggg cacctggtag
     7561 gctttcgcga tgaatggggt ggtgcgggaa gccctcgggt tggcttcaat cacatagacc
     7621 tggtggtcct tgatggcaaa ctggatattg atcaggccgc agatttcgag cgaacgggca
     7681 attttttcgg cgtaaaaggc catggttcgg atttcttctt ccgtgaggct gtacggaggc
     7741 aagagggcat ggctgtcgcc ggaatggatc ccggccggtt cgacgtgttc catgaccccc
     7801 atgatgtgga aatcctcccc gtcaaagata gcgtcgatct ccgcctcctt ggccctttcc
     7861 agaaactggt ctatgagcac cttgttttcc ggcatgtgtt tgaaaatcgt gagcacatgg
     7921 tgttcgagtt cgtcatcgtt gatcacaatg cgcattttct ggccacccag cacataggaa
     7981 ggacggacca ggacggggta gccggtgcgt ttggcaacgg cgagggcctc gtcggcatcg
     8041 cgggcggtgc cgtactccgg gtaggggatt tcaagttcct tgagcagatc ggaaaaacgt
     8101 ccgcggtcct ccgcaatgtc catgctgttg aaggaggtgc cgatgatgcg gatgccctcg
     8161 cggtgaatgt cttccgccag tttgagcgcg gtctgtccgc caagctgaac gatgaccccc
     8221 tccggttttt caaggtccag gatctctttg agatgttccc agtaaaccgg ttcgaagtac
     8281 aacttgtcgg cgatatcgaa gtcggtggat acggtttccg ggttgcaatt caccatgatg
     8341 gattcgtatc ccgcctcctt gatggcgatg aggccatgta cgcaacaata gtcgaattca
     8401 atgccctgtc cgatgcggtt ggggccggag cccaggacga tgattttctt tttatcggac
     8461 gggacggatt cgttctcacg gtcgaaggtc gaatagaaat agggggtcct cgcttcaaat
     8521 tcggcggcgc aggtgtccac catcttgtag gtcctgcgga tgcccagttt catccggtgg
     8581 cggtagactt ctctttcatg gatccggagc agccaggcaa tctggccgtc cgagtaaccc
     8641 aattctttca tctcccggag gaagggggcg ggaatatcct ccgccacatt ataacgcatg
     8701 agttcgtcct cgatgcggac caggcttttg atctgtttga taaaccaggg atcgatgagg
     8761 gttagcttgt gaatcgtttt ttcgggcacc cccagccgga gagcgtcctt tacccggtac
     8821 acgcggtcgt caaaagcggt ttcaagcctt ttaaggatgt cctgggtctg gatccattcc
     8881 tttttatcgg ccccgaggcc catacgccgg ttttcctggg actggcaggc cttctgaagg
     8941 gcttccagga aactgcggcc gatcgccatg acctccccca cggacttcat ctggaggccg
     9001 agcttatggt ccgcacccgg aaatttctca aaatcccaac ggggcatttt gacaatgaca
     9061 taatcgagag tgggctcaaa ataggcggag gtggttttgg tgatctggtt cttcagttca
     9121 tccaggttgt atccgatggc cagtttggcc gcgattttgg caatgggata accggtggct
     9181 ttgctggcca gggccgaaga ccgggagacc ctggggttga tttccactac aatcagttct
     9241 tcgttttcag ggttgagggc aaactgcaca ttgcagcccc cggcaaaatt acccatcgaa
     9301 cgcatgaggg tgatggcatc cgtcctcatt ttctggaatg cggtatccga cagggtcatg
     9361 gctggggcca ccgtaatgct gtcccccgtg tgaatgccca tagggtccat gttttccaca
     9421 gtgcagatga tggcgacgct gtcgttgagg tcccggagca attcgagttc aaattccttc
     9481 cagcccagga ctgctttttc caccagcacc tcgtgggagg gggaagcgtc cagcccgcgg
     9541 cggagggctt catcgaattc ttcttccttc attacaaacc cgcctcccgt gcctcccagg
     9601 gtgaaggaag gccggatcac caggggaaac ccgatcttct gggcggcatt tttcccttcg
     9661 aggaaggaat tggcgatata ggagggggga acggacatgc caagttgttt tacgtggttc
     9721 ctgaacttct cccggttttc cgccagttcg atggcatcga tgtccacccc gatcatgcgt
     9781 actccgtatt tggcccagat gccctgtttt tcggcttcca cggccaggtt cagggcggtc
     9841 tggcccccca tggtaggcaa tacggcgtct attttttgct cctccaggat tttaaccagg
     9901 ctttcgaccg tgagcggcca gagatagacg tggtccgccg tcaccggatc ggtcataatg
     9961 gtggccgggt tggaattgat gagggagacc tcaatcccct cctccctcaa tgacctggaa
    10021 gcctgggacc cggagtaatc gaattcacag gcctgtccaa tgacaatggg gccgctgccg
    10081 atgatcagga cggattttat ggtgtggtca cgaggcatgg atggatcagg tatggttgta
    10141 ttaaaaaatc gcgcaaagat aaaacttagt ggggacctcc gtatggttcc ggccggatta
    10201 atgtcgcttt cccgggggcc tgaaaaccac attttcatac atttggacca gatatgaacc
    10261 cacccaggat attaatcacg gacgaggtcc atcccgtctt gctggacggt ctccgggaaa
    10321 tggcttttga cctggattac cacccggcca ttgcctatga agatgtcctc cagctcattg
    10381 gatcctacga cggcctggtc gtcaacagca aaatcatctg tgaccaaaac tttatcgacc
    10441 gggccgcgcg gttgcggttc attgcccgcc tgggatccgg cagggagatc atagacctgg
    10501 aatacgccgc cagccggggc attcaaacct ttttttctcc cgacggcaac aacaatgcgg
    10561 tcgccgaaca cgcactgggt atgttgctgg ccctggcgaa tcaactggtc aaggcagacc
    10621 ggcaagtccg ccaaaagatc tgggaacggg aggccaggag gggttgggaa ctcagcggca
    10681 agacgattgg catcattgga tttggatata ccggaagcca gtttgcccgc aaactggcag
    10741 ggatggaaat gaaggttctg gtccacgaca aatacctgtc cggcttccag tcccaacttc
    10801 cttttatcgt ggagaccggg aaggaagata tcatccggga agccgatatt atcagttttc
    10861 acctgccctt gtcggcggaa acctaccatt attgcaacga tgacctgatc gggtcctgca
    10921 aacccggggt gatcctgatc aatacctcca ggggtaatgt gatcgatacg aaggccttgg
    10981 tccgcggatt gacctcgggc cgggtgggcg gcgcctgcct agatgttttt gagaatgaaa
    11041 aacccatgac ttttactgcg gaagaggacc ggctttattc caccctttat gccatggacc
    11101 aggtgatcct gagccctcac atcgcaggct ggaccaggga atcaaagtac aagatggcct
    11161 ccatcctgtt gaaaaaaata ttgaatttat accgctccgg ggaccatgaa aaccgctccg
    11221 gggccacgct ttaaaaaagg caggaaattt tccgatgaaa aggtcatttg ttttttcaaa
    11281 aaactacctt tgccacaact ttaaaagaac ccatttggat agatgaagaa ccgttatttt
    11341 gaccttatcg aacaaacctt ctattttccg caggaaggtt tcgatatcga aaacggatac
    11401 cttgatttta acggtatacc cctgatccac ctgattcgga aatacggcac tccgcttaag
    11461 ctgacctacc tgccccgcat cggcgcccag atcaaacggg cccggaacct gttcaaccgg
    11521 tcaatgaaaa acgtgggtta caacgggcaa tactattatt gttattgcac caaatcctgt
    11581 cacttccgct ttatcctcga agaagtgctt caaaacggcg cgcacctgga gacttccagt
    11641 tcattcgata tcgacctcat ccgcaaattg tacatgcaga accagctcga taaggatgag
    11701 atcattattt gcaacgggta caaggccggg gattacggca gaaagatctg caaactgatc
    11761 aatgagggct ttaagaatgt gatccccatc ctggacaata aaaacgaact gggcatttac
    11821 gaggaacatg tggaaaacgg atgcaaaatc gggatccgca ttgccacgga agaagagccc
    11881 aatttccagt tttatacctc acgcctgggg atccgcaaca gcgaggtcat ccagttttac
    11941 aaggagcgcc tggccaacaa ccccaagttt aaactggaaa tgctgcattt tttcgtggat
    12001 accggcatca aggacacgat ctattattgg ggtgaattca aaaaagtcct caagatctat
    12061 tgcgagctca agaaaatttg cccggaactc cgggcgatag acatcggcgg aggcttgccc
    12121 atccgcaatt cactcggttt tgagtacgat tacagctata tgatcaacga gatcgtaaag
    12181 cagatcaaga tcgcctgcca ggacgaaggc attccggaac cggatatttt ttcggaattc
    12241 ggcaaataca ccgtcgggga aagcggggcc acgatctttt ccgtgatcgg ccaaaaacaa
    12301 caaaacgatt ccgagttgtg gtatatgatc gacaattccc tgatgaacac catcccggac
    12361 agctggggca tggccgaacg ttttatcctg ctgcccatca acaaatggta caacgatttt
    12421 acccgggtga atatcggggg catcacctgc gacaattccg acttttacaa ttccgaagtc
    12481 catgaaaacc aggtgtacct gccggctttt tccaagacgg accccgagcc cctttatatc
    12541 gggtttttcc ataccggcgc ctaccaggac gcgcttagcg gatacggagg aatcaaacac
    12601 tgcctgatac ccagccccaa acacatcctg gtggaccggg atgaacaggg caacctggtg
    12661 gaccgggtgt acctggacga acaaaaagcc gacgacatgc tgaagatcct cggctattga
    12721 ttttttaaac cgataaaata ccgagaccat gagccaagga ataatcagcg cttttattga
    12781 aaaacatttc aggcatttta atgcagccgc cctggtggac gcggccaaag ggtaccagca
    12841 tcacctggat atcggcggca aaatgatggt caccctcgcc ggcgccatga gcacggcgga
    12901 attgggcaaa tccctggctg aaatgatccg gcagggcaag gtggacatca tcacctgcac
    12961 cggcgccaac ctggaagaag acatttttaa tctcgtggcc catacccact ataaaaggat
    13021 ccccaattac cgcgacctca ccccggaaca ggaacaggaa ttgctggacc atcactacaa
    13081 ccgtgtgacg gatacctgca ttccggagat ggaagccatg cgccggatgg aacaacagct
    13141 gatcgatgtc tggaacgagg ccaaaaaaaa cggcaaacgt tattttcccc atgaatttgt
    13201 ataccagatc atccgcaaca aatccctgga aaaatattat gaaatcgacc ccgccaacag
    13261 ctggctgatc gccgcctgtg agcgcaacct cccgatcatc gtgccgggat gggaggacag
    13321 caccaccggc aacatgtttg ccggccattg ccttgaacag gagtacgaac cgaccatggt
    13381 ccgtaacggg atcgaataca tgatgttcct ggccgactgg tacctgaaga ataccgaaaa
    13441 gatcggggtg ggttttttcc agatcggtgg cggcatcgcc ggcgatttcc ccatttgtgt
    13501 ggtgcctatg atctaccagg atatgcggag gaccgatgtt aaatgctggg gttatttctg
    13561 tcagatttca gattccacga ccagctacgg atcctattcc ggcgccgtgc cgaatgaaaa
    13621 aatcacctgg ggcaaactgg aagtggatac ccctaaattt atcattgaat cggatgccac
    13681 gattgtcgcc ccgctgatct ttgcccatat cctgggctgg tgatccttcc gtcaaaggat
    13741 tatcctacca aacccgcccg cctggatatt tccagcatgc ggtcgatgga tgcgcgcccc
    13801 tgttcaatca cccattgggg caggatgatt tccggtaact cgtatttcat gcaggtgtac
    13861 agtttctcca gcgtattgcg cttcatatgg ggacattcgt tgcaggcgca ctggttgttg
    13921 ggcggggccg gaataaaggt tttttccggc gatttcagtt ccatctggtg aaggatccca
    13981 gcctccgtgg ccacgataaa ttccctggct gggttggtca tggtgtattt gagcaggccg
    14041 ctggtgcttc cgatatagtc cgccatttcg aggatatggg gctcgcattc gggatgcgcg
    14101 atgaatttgg ccttgggatg cctttccttc agccggacaa tcttttcgtg ggagaagatt
    14161 tcatggacca tgcaggtccc gttccacagg accatatccc tgcccgtttg tttaatgagg
    14221 tatttaccca ggttgatgtc cggggcgaat atgatttttt tgtcgggagg gatgctgttg
    14281 atgacatgaa tggcattggt ggaagtgcag cagatatcgg tgagcgtttt aatttccgcg
    14341 gtacaattga tataactcac cacgacgtgg tccggatatt tagccttgaa tttggcaaaa
    14401 aggtccggag ggcaggaatc cgcgagggaa catccggcct tcaggtctgg caggagcacc
    14461 tttttgtccg gagaaaggat tttggcagtc tccgccataa aatgcacccc ggcgaaaacg
    14521 atgatatccg ctttggtttt ggcggcctgt tgcgaaagac ccaggctgtc gcccacataa
    14581 tcggccaggt cctggatttc ggattcctgg taatagtggg agaggatgac cgcatttttt
    14641 tcctgcttga gtttcaggat ttctgcttcc aggtccaagt ccggactcac cgggatgtgg
    14701 aggtagccgt cgcgctgtaa ttgttccgca gagatagaac tcaaagacat aaatggattt
    14761 ttaataaaag gaacaagacc aacaagggcc gtgatcgttc cgaaattcaa agataaagac
    14821 tattcggcta tcctgccccg atcaggaaga ttgcaggctt tttgccgaag tgcaccaggc
    14881 ccgcggtttt ccatcttttg accgtctgtg ttttgatgta ttcgtcaggc ccggtgatgt
    14941 ctatggccag gcagagcagg gtggtttcag ccaggtttgc caacaaggtc tccagcaggg
    15001 acagattccg gtaaggagtt tcaataaaaa tctgggtgta cccggtccgg accatgtcgg
    15061 cttccactct tttcagccgg gggatcaggt ccggccttcc ggtcgggagg tatccgtgaa
    15121 atacaaattg ctgtccattc aatccactgg ccatgagtgc cagggttatg gatgaaggac
    15181 cgatccaggg aactacccgg atcccctgtt catgagcgag cctgaccacc tggctccccg
    15241 gatcggctat ccccgggcaa cccgcttcag agagcaggcc cgccgaggag cccccgttca
    15301 ggacattgat aagcatcgaa aaatcctgcc cggcctggtg tttgtccatc tccaggaaat
    15361 agcaggcgtc gatatcaaaa cctggtaaaa tctttttgat aaaccttcgt gaggtgcgga
    15421 tattctccac cacgaaaaac cggagtcctt ccagcacgcg gacctgttcc ggaatcaata
    15481 ccccctgata ttcttcatcc aggggtgaag ggatcaggta taaaattccc ggctctccca
    15541 tccggtggta tttataaatg caacgatttt ccggtccagg cggcaaagcc cgcttctttg
    15601 atggcctcgg aaaaagtggg atgcgggtga ctgatccggg caatgtcctc cgcgctggct
    15661 ttgaactcca tcaggctcac ggcctccatg atcaggtccg ctgccctagc gcccaggata
    15721 tgcaccccca ggacttcgtc cgtggaagca tcggacaaga ctttgacaaa accttccgta
    15781 tccatgctgg ctttggcacg tccaagggct ttaaagggga atttgccggt tttgtaattg
    15841 tttccctctt ctttcaattg ctcttccgtc ttgccgacgc cagccagttc gggccaggtg
    15901 tacaccaccc cgggaattaa attataatca atatgcggtt tttggcccgc catgatttcc
    15961 gcgaccagga tgccttcttc agaagccttg tgcgccagca tcgggccccg gactacatcg
    16021 ccgatggcat agatgtgggg cactgccgtt tgcaattgtt cattgaccag gatccggccc
    16081 ttttcatcct ggcttacccc ggctttggcc aggtccaggc cctcggtaaa gggcctcctg
    16141 ccaatagcga caaggcaata atccgattgg agtttaaagg attctttggt gtttttatcc
    16201 gaatagctga cttcaactcc tttcttggat ttgatcaccc cgtccacgct gtggttaagg
    16261 tgaaacacca ttcctttttt ttccagtatt tttttcattt cccgggcgca ttccccgtcc
    16321 attccgggaa ggatgcggtc caggaattct atgacctcga ctttggtgcc cagccttgaa
    16381 aagaccgatc ccagttcaag cccgatcacc ccggccccta tgatcaccat tcggccgggg
    16441 acttcccgga tattcagggc ttccgtactg gtgatgatcc tttctttatc gtaatggaaa
    16501 gccgccggcg tgaggggttt ggatcccgtg gcaataatta ttttttcggc ctggacggtc
    16561 aacaactggc cgtcgctctt tttgatttcc acctcgtgag gccccttgaa tgaagccgtc
    16621 ccgtggaaaa cttcaatctt gttttttttc atcaggaact tgaccccttt tacggtagcg
    16681 tccaccactt cgtcttttct gcggatcatc tggggcatgt ccagatcaat gcctttcacc
    16741 cggagtccgt gggaagcaaa ctgatgcatg gcctggtcgt aatgctcgct gctgtccaaa
    16801 agggccttgg agggaatgca acccacattg aggcaggtgc cccccaggct ggcgtatttt
    16861 tccaccaggg cgactttcat acccaactgt gcgcatcgaa tggccgcaac atatccccct
    16921 ggtccagacc ctatgatgag tatctgaaag tccatggatc aggaatttga aggtggggga
    16981 ttaaaccggt ctttgttttt attgaagggg ggtcttggct ttctactctg gtcctgaggc
    17041 ctgctgctgt tttgtggccg ctcgggatta ttcggtcgct cccgcctggg ctgatcttgt
    17101 ctgggcttgt cctgccttgg accctgtctg ccttggccgc ctctgcggtc cgtctgatcg
    17161 gtccgggacc tttgaggacg gttttgatcc gatggaggcc cggcctgcca gttttggttc
    17221 cgggctccct ggttccggtg gtccctggac ctccctttcc ttcttttttc ctccggcaat
    17281 tcaatctccc cggtcacatc ggcaaatccg gcgtcctcag catccgcttg gccgcctggt
    17341 gcggtcagtg cgacggtttc cccaaaatcc ctggggatct ctccccggtc gtttttataa
    17401 cggatttccc ttacctgttc aggtctgagc ccgaaaacct gggcttcttt attttttccg
    17461 gtggattcca gttcgtaata catgattcc
//
LOCUS       DAOQXT010000102        17098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3755_length_17098_cov_5.920613, whole genome shotgun sequence.
ACCESSION   DAOQXT010000102 DAOQXT010000000
VERSION     DAOQXT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17098
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_17098_cov_5.920613"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKM27_15315"
     CDS             complement(<1..685)
                     /locus_tag="PKM27_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR08687.1"
                     /translation="MNYAVDSKGFYGIFGGAYIPEMLQYNVEQLRSEYLKIIHEPAFQ
                     SEFNYLLKHYVGRPSPLYEAKRLSDKYKARIFLKREDLNHTGAHKINNTIGQILLAER
                     LGKKRIIAETGAGQHGVATATVCALKNLECVVYMGSVDIERQAPNVARMRMLGARVVP
                     ATSGSQTLKDATNEAIRDWIQHPEDTHYIIGSVVGPHPYPDMVARFQSVISAEIREQL
                     PEYTGSPDPD"
     gene            complement(669..1292)
                     /locus_tag="PKM27_15320"
     CDS             complement(669..1292)
                     /locus_tag="PKM27_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNR08688.1"
                     /translation="MKPDLLIKVCGMKLPEQIHALAGLEVDYIGLIFHQASPRFLDLD
                     IDPLFFGNKKKTGVFVNAPYEQILYTQSRYQLDAVQLHGNEPPEFCTRIQDRGIEVIK
                     AFSIDRNYDFERTAEFDGYADLFVFDARGDAPGGNGVRFDWGKLSEYQGATPFLLSGG
                     ISYEHLDEIRHFNHPACIGIDINSRFEHGPGNKDLITIKKFVYELRR"
     gene            complement(1282..2079)
                     /gene="trpC"
                     /locus_tag="PKM27_15325"
     CDS             complement(1282..2079)
                     /gene="trpC"
                     /locus_tag="PKM27_15325"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNR08689.1"
                     /translation="MNVLQQIVDNKRRELETAKSRISLEQLKAGPHFDRPCLSLAKSL
                     QEKAGIIAEFKRRSPSKGVIRHPASAAEITRSYYLHGASACSILTDHDFFGGSLQDML
                     DARPGLEIPVLRKEFILDPYQVYEAKSAGADLILLIAECLSREEIRDLSRLAHDLGME
                     VLLEMHLESELDKWIPGIDLLGINNRDLRTFDTGIEKSLKMAALLPAEAVWVSESGLK
                     GEDELHELSTAGYRGFLIGETFMKAADPGALCGAFVNSLKTTPHHEA"
     gene            complement(2076..3074)
                     /gene="trpD"
                     /locus_tag="PKM27_15330"
     CDS             complement(2076..3074)
                     /gene="trpD"
                     /locus_tag="PKM27_15330"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNR08690.1"
                     /translation="MKKILTYLFEHKKLSRAEAREILVNISRNVYNEAQVAAFITVYL
                     MRAISEEELQGFVDALLEMAVPADFEGQETIDLCGTGGDGKNTFNISTLAAFVVAGAG
                     YKVTKHGNSGVSSKCGSSDVLETLGYRFTNDPDTLKRQLDKSNICFLHAPLFHPAMKT
                     VAPIRKQLGVKTFFNMLGPLVNPAKPKYQMTGVFNLELARLYAYLFQKQAGKRFAVLH
                     ALDGYDEISLTGNFKVKTHSSDRILAPADLGMKTVRQEDIHGGDTREDAAAIFLHVLK
                     GAGSPAQQAVVAANAAIAIQTFSPALSLRDAVAAAEESVKSGRAFQSFKNLIEAVA"
     gene            complement(3095..3706)
                     /locus_tag="PKM27_15335"
     CDS             complement(3095..3706)
                     /locus_tag="PKM27_15335"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNR08691.1"
                     /translation="MKILMIDNYDSFTYNLVQYLRELTGKSIQVVRNDEVPASYAADF
                     DCIFISPGPGVPSESGIILEVIRTFGPSKSIFGVCLGLQAIGEAYGGRLVNLAQVYHG
                     VNTPVYIRDADEPIFKGIPSPFLAGRYHSWVVDQDQVPDSLVVTAVDGAGMIMAASHK
                     TYHVKGVQFHPESILTPDGKKILANFLVESESLFKKQLIEDTF"
     gene            complement(3714..5120)
                     /locus_tag="PKM27_15340"
     CDS             complement(3714..5120)
                     /locus_tag="PKM27_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNR08692.1"
                     /translation="MEKIKLYVHSRKYLADTVTPVHLYLKLRDHFDESGLLESTDFRS
                     IENCHSYLGLGKLAAITVNVGTMKVELPGGESHEHKLSGPHDLSERLREFMDRFDLQQ
                     QGDYQLLNGLFGHFNFDAIQYFDTITFDPAKRTTDIPDVRLTLYKFLIAIDHFKDEMY
                     IMENSLDGQSNGIEYLDSLILNEQFNAFRFQKVGEESSSLTDQEYRDLVTKGKYHCQI
                     GDVFQVVFARRFRQGFAGDDFNVYRALRSVNPSPYLFYFDMGNYRIFGSSPESQLVIK
                     NGIASVNPIAGTYKRTGNDEQDQQRAKELALDPKENAEHIMLVDLARNDLGVHSDKVE
                     VRVLKEVQYFSHVIHLVSKVDGRLSKGHNIFKVFGDTFPAGTLSGAPKYKAIELINAY
                     EPEHRNFYGGSIGFAGFDGSLNHAITIRSFLSKDQVLHYQAGAGIVFTSVEENELQEV
                     NNKLGALRKAMTEAQNIK"
     gene            complement(5335..>6279)
                     /gene="holA"
                     /locus_tag="PKM27_15345"
     CDS             complement(5335..>6279)
                     /gene="holA"
                     /locus_tag="PKM27_15345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNR08693.1"
                     /translation="LLHGEEPYFIDQISDFFQDQLLNETEQVFNRRVFYGKETDFKTV
                     LDEARQYPMMSSRRLVLVREAQEMKGMDEMVGYFENPVASTILVLCYKYKKFDQRTRA
                     AKAIKQHGMVFESKPLYDNQVPDWILKEAFRRGIKIAPDACQLMAEYLGTDLGSIVQA
                     IDKIVISATPGEVVTAHDLDDFIGIHKDFNVFELQKAIGQRDTVKAFRIIHYFEANPK
                     ASPMVMILANLYAYLAKLFAARAANARNDQDLMAALQLKSPFFLKEYKEALKHFSLPQ
                     LEEALLLLREYDLKYKGVNSKGVEEGALLKELVSRILL"
     assembly_gap    6280..6379
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6413..7183
                     /gene="surE"
                     /locus_tag="PKM27_15350"
     CDS             6413..7183
                     /gene="surE"
                     /locus_tag="PKM27_15350"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNR08694.1"
                     /translation="MPRPLILVTNDDGLFAPGIRALVKVAAEFGEIVIVAPNSPQSGQ
                     GHAVTLESPLRINRVNTFGDIEAYECTGTPVDCVKLAKHLVLRGRKVNLCLSGINHGS
                     NSSINIIYSGTMSAAMEASIEGIDSIGFSLLDFSFDADFESSEFYVRHIIEKTLRHGL
                     THTNLLNVNIPKLKKSEIRGIRVCRQAEGRWQEEFMEGTDPRGQKYYWLTGKFVTRDA
                     LEDTDEWALHNGYISVVPSGHNLTRYQAIEPLRFLENV"
     gene            7176..7496
                     /locus_tag="PKM27_15355"
     CDS             7176..7496
                     /locus_tag="PKM27_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08695.1"
                     /translation="MSDLLARIFRRDQWINGLVSGLFLPIVLFAGLLYLSESIGKMAT
                     GNENQDLHLLRPRTLALLALFANMILMQAFKKLRWNQSMRGMAVSTFICVALWVIKFA
                     GELF"
     gene            7518..8621
                     /gene="lpxB"
                     /locus_tag="PKM27_15360"
     CDS             7518..8621
                     /gene="lpxB"
                     /locus_tag="PKM27_15360"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNR08696.1"
                     /translation="MKYYILSGEASGDLHGSNLVKQIRSLDQDAEIRAWGGDLMEAAG
                     AKIVKHYKDLAFMGFVEVIQNIRTIHRNFKLCKEDLLHFKPHALILIDYPGFNLRIAE
                     WAKKNGFRIFYYISPQLWAWKPGRVKIIRSCVEQLFTILPFENEFYKKYQVFPEYVGH
                     PLLESTGSFKPDKMIAEELKGKKVLALLPGSRKQEITRILPVLLRALDAFPEYTPVIA
                     AAPAQPLSLYEGIANDQGKAGIRIFHHHTYSILSLASLAWVSSGTATLETALFRVPQV
                     VCYAGNPLSYHIAKRLIRVKYISLVNLILDYPLLPELIQHDLTPDKLIDATQKLDPAL
                     IAKEYGKLHDLLQPANASARVAASIINKLQFKA"
     assembly_gap    8764..8863
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8864..9258
                     /locus_tag="PKM27_15365"
     CDS             <8864..9258
                     /locus_tag="PKM27_15365"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNR08697.1"
                     /translation="EKADTTRDPDLSETGRQRAEKLASLLSNENIKHIYSTNYKRTRQ
                     TAAPLSLINGAEILLYTPDSLNALADKLRHLEGNVLVVGHSNSTLSLMDAMGIKYSLP
                     KIEESDYSNLFKIRKKGKRFTGLEEMKF"
     gene            complement(9270..9719)
                     /locus_tag="PKM27_15370"
     CDS             complement(9270..9719)
                     /locus_tag="PKM27_15370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNR08698.1"
                     /translation="MKLTAFVLGALVILSFTRCLEPSQYEQDDDIIQDYIKNKNLQMV
                     KHETGLYYQIINSGSGETPELSDSVSVHYKGFLTNGQQFDGTTTSPATFLLGELITGW
                     KIGIPLVKEGGEIMLLVPSNLGYGSISVGPIPANSVLVFEVELVDVK"
     gene            complement(9732..11156)
                     /locus_tag="PKM27_15375"
     CDS             complement(9732..11156)
                     /locus_tag="PKM27_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR08699.1"
                     /translation="MIFSLSPSEKKIFQVVRESAASLGIPCYVVGGYVRDKLLGRASP
                     DIDFLAVGNGIALAQEVAGRLYPIPRISVFQRFGTAQIIHGEFDLEFVGARKESYRED
                     SRKPMVEDGSLEDDLRRRDFTINAMAVSLNDEDFGQLIDLFDGMKHLEMKLIKTPLEP
                     ERTFSDDPLRMLRAVRFASQLEYTIDAFTFRGIQQTADRISIISMERICTELEKILCS
                     RQPSTGFKLLHLAGLLKYILPELTALEGVEYKDGKGHKDNFYHTLMVLDNVAAKSENI
                     WLRWAALFHDIGKPATKRFNGKQGWTFHGHDLLGASMLPRIFKRLRLPGDHRLKYVQN
                     LVRLHLRPISLTKEEITDSAIRRVLFEAGEDVDDLMILCEADITSKKPEKVRTILENY
                     QIVRSRMLEIEQKDQLRNWQPPIDGQEIMDTFGLKPSLQVGQIKTAIREAILDGKIDN
                     DYQQARTFMLEEAARMGLKPLQSI"
     gene            complement(11178..12179)
                     /gene="dusB"
                     /locus_tag="PKM27_15380"
     CDS             complement(11178..12179)
                     /gene="dusB"
                     /locus_tag="PKM27_15380"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNR08700.1"
                     /translation="MVKIADIELGDFPILLAPMEDVSDPPFRALCKMQGCDLLYTEFI
                     SVEGLIRDAAKSLQKLDIYPEERPIGIQIFGGEMDSMLRAIPLVEEAGPEILDINYGC
                     PVHNVVCRDAGAGILKDIPKMVALTKAVVENTRLPVTVKTRLGWDANTIKIDEVVRRL
                     QDTGIQAISIHARTRSQMYKGEADWTHIARIKNDPSIFIPVFGNGDIDSPQKAKEYKE
                     RYGPDGIMIGRASIGHPWIFREIKHYLRTGEILDPPSLDERIEAVKAHLDFSVRWKGP
                     KLGVLEMRRHYKNYFRGIEGAKSFRERLVLEPESQGVLEILEELHGQLMQSRESLPV"
     gene            12268..13581
                     /gene="nhaD"
                     /locus_tag="PKM27_15385"
     CDS             12268..13581
                     /gene="nhaD"
                     /locus_tag="PKM27_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNR08701.1"
                     /translation="MEMLVIVCFIVGYLLIVFEHPLKLDKTIPALLMGILCWTFISIG
                     GLNVFGAEEAGHHDVNNVVLHHLSSIAAILIFLIGAMTIVELVDLHKGFAVITNRIRT
                     TDKRKLLWIISWLAFFLSSVLDNLTTTIVFVSLLRKLIRDRNERIWYVSFVIIAANAG
                     GAWTPIGDVTTTMLWIAGKVSTYLLIVNLAIPSVICLALPLFIASFLKVFQGQISSMH
                     SADSRDLEREKLLSSKTMLIAGVGALIFVPIFKTVTHLPPWMGMMLGLSIVWLISEYI
                     HPEEDFSEDRRHKYTVRHALSRIELSSILFFLGILMAISALESLGKLAQLAAYMDRVI
                     PNQDVVATVIGIMSAVIDNVPLVAATMGMYELPMDAKMWHFIAFSAGTGGSMLIIGSA
                     AGVAAMGMEKIDFIWYLKRIAWLAAIGFFAGCLATSLIHLILHEA"
     gene            13671..14978
                     /gene="nhaD"
                     /locus_tag="PKM27_15390"
     CDS             13671..14978
                     /gene="nhaD"
                     /locus_tag="PKM27_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNR08702.1"
                     /translation="MEWLIIVSFVIGYLLIVFEHPLKLDKAIPALFMGIFIWSVIALN
                     HMPLIDVSGDHGTTEAVAHHLGKITEILLFLLGAMTIVEVVDAHHGFSVITNRIRTKN
                     KTHLLWIISALAFVLSSILDNLTTTIVLVSLLRKLIYRREERIWYVAFVVIAANAGGA
                     WSPIGDVTTTMLWIAGKVSSVQLIINLIVPSILCMAIPLIVASRMKIFKGNLEAAEDD
                     EGYYSSKSSGIQSSKIMLMVGVGALVFVPIFKSVTHLPPWMGVMFGLGIVWLVTEFVE
                     PELDLDEQDRMKYTLRHALGRVELSSILFFLGILLAISGLESLGILKDLAVKMDAIIP
                     SQDLVATLIGILSAIIDNVPLVAAAIGMYEVPLDHELWHFIAFSAGTGGSMLVIGSAA
                     GVAAMGMEKIDFIWYLKKISGLAALGFLGGCIYLALVNYLRAA"
     gene            15008..16081
                     /locus_tag="PKM27_15395"
     CDS             15008..16081
                     /locus_tag="PKM27_15395"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HNR08703.1"
                     /translation="MLRFNRTQSGLLSLVALIILFSCKPSAKLQVLQPADLVIPEHIR
                     KIVLIDRSKPAGGFWSNVESVLTGEEYQQDKQGRRRAMEGLSELLQKTPRFATIYSNV
                     EMTGSKGGREFMTPLSWREVEEICNKFGGDALVAIEMFDSDIESSTTSRSVKEKDKEG
                     KEITRVVFDGRRRGTVKLGWRFYDPKDRTIVDEYRDNDTEEVTSSGAASQQAATNNLR
                     KSYELVRDIAFDLGGKYGKRIAPVWITLSRTYYKNIKGANEDKFEQAARYADAGEWAK
                     AAEIWEKVASVRSNPDAAGKATYNLAVAAEVNGQLNTALQYARDAYTQFSDKKAKNYI
                     RLLEQRIQDLETIQRQMHEKKTS"
BASE COUNT         3842 a         4471 c         4408 g         4177 t
ORIGIN      
        1 tatccgggtc cggtgaaccg gtgtattcgg gcaattgctc ccggatctcc gcgctgatca
       61 cactctgaaa gcgggctacc atatccgggt aaggatgagg ccccaccacg gagccgataa
      121 tataatgcgt gtcttccggg tgttggatcc agtcacggat cgcttcattg gtggcgtctt
      181 tcagggtttg ggacccgctg gtcgccggga ccacccttgc ccccagcatc cgcatgcggg
      241 ccacattagg cgcttgtctt tcaatgtcca ccgagcccat gtacaccaca cattcaaggt
      301 ttttgagggc gcatacggtg gccgtggcta cgccgtgctg cccggctccc gtttcggcga
      361 tgatccgttt tttacccaac cgctccgcca ggaggatctg gcctatcgta ttatttattt
      421 tatgggcgcc ggtatggttc aggtcttccc gtttaagaaa aatgcgggcc ttgtatttat
      481 ccgaaagccg tttggcttcg tacagcgggc taggcctgcc cacataatgt ttcaacaggt
      541 agttgaattc cgactggaag gcgggctcat ggatgatttt aaggtattcc gaccggagtt
      601 gctccacatt atattgcagc atctccggaa tatacgctcc cccgaatatc ccataaaacc
      661 ctttggaatc aacggcgtaa ttcatacaca aattttttta tcgttatgag gtctttgttc
      721 cccggcccgt gttcaaaacg gctgttgata tcgatgccta tgcaggcggg atgattaaaa
      781 tgacggatct catccaggtg ttcgtaagag atgcctccgc tgagcaggaa tggggtggct
      841 ccctggtatt cggaaagttt tccccagtcg aaacggaccc cgttgccgcc cggcgcatca
      901 ccccgggcat caaaaacaaa aagatcggca taaccgtcaa actccgccgt tctttcaaaa
      961 tcataattgc ggtctatgga gaaagccttg atcacttcga tgccccggtc ctggatgcgg
     1021 gtacaaaatt cagggggctc gttgccatgc aattgaaccg catccaattg gtaccggctt
     1081 tgggtgtaca ggatttgttc atagggcgcg ttgacaaaga ccccggtttt ctttttattg
     1141 ccaaaaaaca agggatcgat gtccaggtcc aggaaccggg gtgaggcctg gtgaaagatc
     1201 agtccgatgt aatccacctc caggccggcc agggcatgga tctgttcggg cagtttcatg
     1261 ccgcagactt taattaaaag atcaggcttc atggtggggg gtggttttga ggctgttgac
     1321 gaaggcgccg catagggctc ccggatcagc ggctttcata aaggtttcac cgatcagaaa
     1381 accccggtac ccggccgtgg ataattcatg caattcatct tcccccttca acccgctttc
     1441 cgatacccat accgcttctg cgggcaataa ggcagccatt ttcaatgatt tttcgatacc
     1501 cgtatcaaag gttctaaggt cccggttgtt gatgcccagc aaatcgatcc ccggaatcca
     1561 tttgtccagt tctgattcca aatgcatttc caacaggact tccatgccga gatcgtgagc
     1621 aagccggctg agatccctga tctcctcccg gctaagacat tcggcgatca ggaggatcag
     1681 gtctgcaccc gcggatttgg cttcatagac ctggtagggg tccaggataa attctttcct
     1741 caataccggg atttcaaggc cggggcgcgc atccagcata tcctgcaggg aaccgccgaa
     1801 aaaatcatga tcggtcagga tcgaacaggc gctggcgccg tgaaggtaat aggaccgggt
     1861 gatctccgcg gcagatgcgg gatggcgaat gacccctttg gacggggacc ttcttttgaa
     1921 ttccgcaatg attcccgctt tttcctgcag gcttttcgcc agggaaaggc atggccggtc
     1981 aaaatgggga cctgccttca gttgctccag gcttatccgg gacttggcgg tttccagctc
     2041 cctccgcttg ttgtccacga tttgttgcaa tacgttcatg ctacggcttc gatcaggttt
     2101 ttaaaggatt ggaaagccct gccgcttttt acactttctt ccgccgccgc aaccgcatcc
     2161 ctcaggctga gcgccgggct aaaggtctgg atggcgatgg ccgcattggc ggcaacgacg
     2221 gcttgctggg ccggactgcc cgcccctttc agcacatgga gaaagatcgc cgccgcatct
     2281 tcacgggtat cgccgccgtg aatatcttcc tgtctgaccg ttttcatccc gaggtcggcc
     2341 ggagcaagga tccggtccga ggaatgggtc ttgaccttaa aattaccggt gagggaaatc
     2401 tcgtcgtatc catccagggc gtgcagtact gcaaaacgtt tcccggcttg tttctgaaac
     2461 agataggcgt ataaacgggc cagttcaaga ttgaacaccc cggtcatctg atattttggt
     2521 tttgccgggt tgaccagggg cccgagcata ttaaaaaagg ttttgacccc aagttgtttc
     2581 cggatcgggg ctacggtttt catcgcagga tgaaacaggg gtgcatgtaa aaagcagata
     2641 ttgcttttgt ccagttgtcg tttgagggta tccgggtcgt tggtaaaccg gtatccgagg
     2701 gtctccagga catcggaaga accgcatttg gaactcacgc cggaattgcc gtgcttggtg
     2761 accttgtatc cggccccggc aaccacaaaa gccgccaggg tggaaatatt gaaggtgttt
     2821 tttccatcgc ctccggtacc gcagaggtca atggtttcct gcccttcaaa gtccgcaggg
     2881 accgccattt ccagcaaggc gtcgacaaag ccctgcaatt cttcctccga aatggcgcgc
     2941 atgaggtaga ccgtgatgaa ggcggctacc tgggcttcat tgtaaacgtt ccgggagata
     3001 ttgaccagga tttccctggc ttccgcccgg gataattttt tgtgttcaaa aaggtaggta
     3061 aggatttttt tcatggaatg gagattgatg catttcagaa ggtatcttct atcaattgtt
     3121 ttttgaataa ggactcgctt tccaccagga aattggccag tattttttta ccatccgggg
     3181 taaggatgga ttcgggatga aactgcacgc ccttcacatg gtaggtcttg tggctggcgg
     3241 ccatgatcat gccggcccca tccactgcag tgaccaccag cgaatccgga acctggtcct
     3301 gatcgaccac ccaggaatga taccttccgg ccaggaaggg ggaagggatc cccttaaata
     3361 tcggttcgtc cgcatccctg atatataccg gggtgttcac tccatgatat acctgggcga
     3421 ggttgaccag acggccgcca taagcttccc cgatcgcctg gagtcccagg cagacgccga
     3481 agatggattt ggaaggtccg aaggtgcgaa ttacctccag gataatcccc gattcagagg
     3541 gaaccccggg acccggcgaa ataaaaatgc aatcgaaatc cgccgcatag gaagccggca
     3601 cctcatcgtt gcgcaccacc tggatggatt taccggtcag ctcacgcaaa tattgcacga
     3661 ggttgtaggt aaaggaatcg taattgtcga tcatcagtat tttcatgctc cgtctatttg
     3721 atgttttggg cttccgtcat ggcttttcgg agggcgccca acttgttgtt tacttcctgc
     3781 agttcgtttt cctcgacgct ggtgaatacg atgccggcac ccgcctggta gtggaggacc
     3841 tggtccttgc tcagaaacga acggatggtg atggcgtgat tgagcgatcc gtcaaagccg
     3901 gcaaaaccga tggatccccc gtaaaaattg cggtgttccg gttcgtaggc gttgatgagt
     3961 tcaatggcct tgtatttcgg ggctcccgat aaggttcctg cgggaaaggt atccccaaac
     4021 actttaaaaa tattgtgccc cttcgagagc cggccatcca ctttggatac caggtgtatg
     4081 acatggctga aatattgcac ctccttgagc acccggactt ccaccttgtc ggaatgcacg
     4141 ccgaggtcgt tgcgggccag gtccaccaac atgatgtgct ccgcattttc tttcgggtcg
     4201 agcgccaatt ccttggcccg ttgctggtcc tgttcgtcgt tgccggttct cttataggtg
     4261 ccggcgatcg gattgacgct ggcaatcccg tttttgatca ccaactggga ttcgggcgat
     4321 gaaccgaaaa tgcggtagtt gcccatgtca aaataaaaca ggtaaggaga agggttcacc
     4381 gaacgcaggg cccggtaaac attaaaatca tctcccgcaa aaccctgccg gaaccttcgg
     4441 gcaaatacca cctggaatac atccccgatc tgacaatggt atttgccttt ggtcacgagg
     4501 tcgcggtatt cctggtcggt caatgaactg ctttcctccc ctactttttg gaatcggaac
     4561 gcgttgaact gttcattcag gatcagggaa tcgaggtact caatcccgtt ggactggccg
     4621 tccaggctgt tctccataat gtacatttca tccttaaaat ggtcgatggc gatgaggaat
     4681 ttataaaggg tgagccggac atcggggata tcggtggttc gtttggccgg gtcaaaggtg
     4741 atggtatcga aatattggat agcatcaaag ttaaagtgcc cgaaaaggcc gttgagcagt
     4801 tggtaatccc cttgctgctg caggtcaaac cggtccataa attcccggag ccgttccgac
     4861 aggtcatggg gcccggacag tttgtgttca tggctttcgc ccccgggcag ttccactttc
     4921 atggtcccca cattgacggt aatggcggcc agtttgccga ggccaaggta ggaatggcaa
     4981 ttctcaatgc tcctgaagtc ggtgctttcg aggagtcccg attcgtcgaa atggtccctc
     5041 agctttaaat acaaatgcac cggggtcacc gtgtcggcca ggtatttccg gctatgaacg
     5101 tatagtttta ttttttccat tttccacaaa ctgaaagcgg cctgccgggt gcaccgacaa
     5161 gccgctttgg ttatatgcta ttttaacaaa caactggctt gctcagacca cccggggatc
     5221 agggacctgc caccaccagc tattgttagt ttttttcatt ttatttactt cggaaggcaa
     5281 atataatgcc gtttttgaat tgtcctaaga cgtttaaaaa aatttaaaca ttgtttaaag
     5341 caggatgcgg ctgaccaatt ctttcaacag ggccccctct tcgacccctt tgctgttgac
     5401 ccccttgtat ttaaggtcgt attcccggag caggagcaag gcttcctcga gttggggcag
     5461 gctgaagtgt ttcagggcct ccttgtattc ttttagaaaa aaaggacttt tcaattgcag
     5521 ggcggccatc aggtcctgat cgtttctggc attggcggca cgggcggcaa acaatttcgc
     5581 gagataggca tagaggttgg ccaggatcat caccatgggg ctggctttgg ggttggcttc
     5641 aaaataatgg atgatccgga aggcctttac ggtatcccgc tgccctatgg ctttctgcaa
     5701 ttcgaaaaca ttaaaatcct tatggatgcc gataaaatca tcgaggtcat gggccgtgac
     5761 cacttcaccg ggggtggcgg agatcacgat cttgtcgatg gcctgtacga tggagcccag
     5821 gtcggtgccc agatattcgg ccatcagctg gcaggcatcc ggagcaattt tgatgcctct
     5881 gcggaaagcc tctttcagga tccagtccgg gacctggtta tcatacaggg gtttggattc
     5941 aaagaccatc ccgtgctgtt tgatggcttt ggcggcccgg gtgcgctgat caaacttttt
     6001 gtacttataa cacaaaacga ggatggtcga ggccaccggg ttttcaaaat aacctaccat
     6061 ttcatccatg cccttcattt cctgggcctc ccggacgagg accaggcggc ggctggacat
     6121 cataggatat tgccgggctt catcgagcac ggttttaaaa tcagtttcct tcccgtaaaa
     6181 taccctccga ttaaaaacct gttcggtttc attcagcagc tgatcctgga aaaagtcgga
     6241 aatctgatca ataaaataag gttcttcccc atggagcagn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnt cccccgcaaa ccttattttt gccccctgat ctatgcccag
     6421 accacttatc cttgtgacca atgacgatgg actgtttgcc ccggggatcc gggccctggt
     6481 taaagtcgcc gccgaattcg gggaaatcgt aatcgtcgcc cccaacagcc cccaatccgg
     6541 acagggccat gcggtaacgc ttgaatcccc tttacggatt aaccgtgtga ataccttcgg
     6601 agacatcgaa gcgtatgaat gcaccggcac cccggtcgac tgcgtaaaac tggccaaaca
     6661 cctggtgctg cgcggccgga aggtgaacct ctgcctctcg ggaatcaatc acggatccaa
     6721 ttcgtccatc aacatcattt attccgggac gatgtccgcg gccatggagg cctccattga
     6781 agggattgat tcgatcggtt tttctctgct ggacttttcc tttgacgccg attttgaatc
     6841 ttccgaattt tacgtcaggc atattattga aaagaccctt cgtcatggcc ttacccatac
     6901 caacctgctg aatgtaaaca ttcccaaact gaaaaaatcg gagatccggg gtatccgcgt
     6961 ctgtcgccag gcggaaggaa gatggcagga ggaatttatg gaagggaccg atccgagggg
     7021 gcagaaatat tattggctga ccggaaaatt cgtcacccgg gatgccctgg aagacacgga
     7081 tgaatgggcc ctgcacaacg gttatatctc cgttgtccct tcaggccaca acctcacccg
     7141 gtaccaggcc attgaacccc ttagatttct tgaaaatgtc tgatctcctg gcaaggatat
     7201 tccggcgcga ccagtggatc aacgggctgg tctccggcct ctttctcccc atcgtgctct
     7261 ttgccggttt gctttacctt tccgaatcca ttggaaaaat ggcgacggga aatgaaaacc
     7321 aggatctcca tctgcttcgg cccaggaccc tggccctgct ggcccttttt gccaatatga
     7381 tcctcatgca agcgttcaaa aaactgcgct ggaatcagag catgcgggga atggccgtat
     7441 cgacctttat ctgcgtggct ttatgggtca ttaaattcgc aggcgaatta ttctgatcac
     7501 caagtcatgg ccgctcaatg aaatattata tcctgtccgg cgaagcttcc ggcgacctgc
     7561 atgggtccaa cctggtaaaa caaatccgga gcctggacca ggacgcggaa atccgggcct
     7621 ggggaggcga cctgatggaa gcggccggtg caaaaatcgt caaacattac aaggacctcg
     7681 ccttcatggg ctttgtggaa gtcatccaga atatccggac catccaccgc aattttaagc
     7741 tctgcaagga agacctccta catttcaagc cccatgccct gatcctgatc gattacccgg
     7801 ggttcaacct gcggatcgcg gaatgggcca aaaaaaacgg cttccggata ttctattata
     7861 tatcccctca actctgggcc tggaaacccg gaagggtgaa gatcatccgg tcctgcgtcg
     7921 aacaattgtt taccatcctt ccttttgaaa atgaatttta taaaaaatac caggtctttc
     7981 cggaatatgt aggccatccc ctcctggaaa gcaccggcag ttttaagccg gacaagatga
     8041 tcgcggagga attaaaaggt aaaaaagtcc tggccttatt gccgggaagc cggaaacagg
     8101 agatcacccg gatcctcccg gtcctgctcc gcgcccttga cgcattccct gagtacactc
     8161 cggtcattgc tgcggccccc gcccagcccc tgtccctgta tgaggggatc gcgaatgacc
     8221 agggcaaggc cggcatccgg atcttccacc atcatacgta ttccatattg tccctggcca
     8281 gcctggcctg ggtgagttcg ggaacagcca ccctggagac ggcgcttttc cgcgttcccc
     8341 aggtcgtttg ttatgccggc aatccccttt cctatcatat cgccaaaagg ctgatccgtg
     8401 taaaatatat atccctggtc aacctgatcc tcgattatcc cctcctgcct gaactcatac
     8461 agcacgacct gaccccggac aagctgatcg atgccaccca aaaactggat ccggctttga
     8521 tcgcaaaaga atacggaaaa ttacacgacc tgcttcaacc ggccaacgcc tctgcgcggg
     8581 tggcggcttc catcatcaat aaacttcaat ttaaagcatg aaaaaatttc caagcctcct
     8641 cctttttctt ccaatctggt tggtgttttc ctgccagaaa tcatcccaga tctacctggt
     8701 ccgtcatgcg gaaaaggccg acacgacccg ggacccagac ctgagcgaaa ccgggcgcca
     8761 aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggaaaa ggccgacacg
     8881 acccgggacc cagacctgag cgaaaccggg cgccaaagag ccgaaaagct ggccagcctt
     8941 ttatccaacg aaaacataaa acacatttat tccacaaatt ataaaaggac caggcaaacc
     9001 gccgctccgc tgagccttat aaacggagcg gagatcctgc tttacactcc ggattcctta
     9061 aatgcgctcg ccgataaact gcgccatctg gaaggcaatg tcctggtggt cgggcatagc
     9121 aattccaccc tcagcctgat ggatgccatg ggtataaagt acagcttacc gaaaatcgaa
     9181 gaatcggatt attccaactt gtttaaaatc aggaaaaaag gtaaaagatt taccggcctg
     9241 gaagaaatga agttttaaat tataataaac tacttcacgt ctaccagttc cacctcaaag
     9301 accagcacac tgttggcggg aatcgggcca accgaaatag atccataacc caggttgctg
     9361 ggcaccagca acatgatctc ccccccttct ttgaccagtg gaatgccgat cttccagccc
     9421 gtgatgagct caccaagcaa aaaggtcgca ggactggtgg tggttccgtc aaattgctgg
     9481 ccattggtca gaaacccctt gtagtgaacg gatacgctgt cactcaattc cggagtttct
     9541 ccgcttccgg agttgatgat ctggtaataa aggccggtct catgtttgac catctggagg
     9601 tttttgtttt tgatataatc ctggatgatg tcgtcatcct gttcatattg gctgggctcc
     9661 aggcaacggg tgaagctgag gatgaccagg gcgcctaaaa caaatgcagt taatttcatg
     9721 gtatggatga gttagataga ttgtaacggt ttaaggccca tgcgggctgc ttcttcgagc
     9781 ataaaagttc ttgcctgctg gtaatcgttg tcgattttac catccaggat ggcttcccgg
     9841 atcgcggttt tgatctgtcc gacctggagg gagggcttca ggccaaaggt gtccatgatt
     9901 tcctggccgt cgatgggagg ttgccagttg cggagctggt ctttttgttc gatctccaac
     9961 atcctggagc ggacgatctg gtaattttcc aggatggtcc tgaccttttc gggtttttta
    10021 ctggtgatat ctgcttcaca gaggatcatc aggtcatcca cgtcctcccc ggcttcaaag
    10081 agcacgcggc gtatggcgct gtcggtgatc tcctctttgg tcaggctgat cgggcgcagg
    10141 tgaaggcgga ccaggttttg aacatatttc aaccggtggt ccccgggaag ccgcagtctt
    10201 ttgaatatac ggggcaacat gcttgccccc agcaggtcgt ggccatgaaa ggtccagccc
    10261 tgcttgccgt tgaacctctt ggtcgccggc tttccaatgt catgaaagag cgccgcccag
    10321 cgaagccata tattctccga tttggcggct acattgtcca ggaccatgag ggtgtgataa
    10381 aaattatcct tatggccttt gccatccttg tattccaccc cttcgagggc ggtgagttca
    10441 ggcaggatgt atttaagaag gcctgccagg tgcaaaagct tgaacccggt ggaaggctgc
    10501 cgggagcaaa ggattttttc cagttctgtg cagatccgct ccatggaaat gatgctgatc
    10561 cggtcagcgg tctgttgaat gccccggaaa gtaaacgcat cgatcgtgta ttcgagctgg
    10621 ctggcaaacc gcacggcccg gagcatgcgc aacggatcat cggaaaaggt gcgctccggc
    10681 tccagcggcg ttttgatcag tttcatctcc aggtgtttca taccgtcgaa aaggtctatc
    10741 aactgcccga aatcctcatc gttgaggctg accgccatgg cattgatggt aaagtccctg
    10801 cgccggaggt catcctccag gctgccgtcc tccaccatgg gtttcctgga atcttcccgg
    10861 tacgactctt tacgggcgcc tacgaattca aggtcaaact ccccgtggat gatctgcgcg
    10921 gtgccaaaac gctgaaatac gctgatccgg ggaatggggt aaagccttcc cgccacttcc
    10981 tgggccaggg cgatcccatt gcctaccgcc agaaaatcaa tatcgggaga agcccgcccc
    11041 agcaatttgt cgcggacata acctcctacc acataacaag gaatccccag gctggccgcg
    11101 gattcccgga cgacctggaa tatctttttt tcagaagggc tgaggctgaa gatcacggtt
    11161 accggttgtt cagcgggtta aacaggaagg gattcccggc tttgcatgag ctgtccatga
    11221 agctcttcga ggatctcgag gaccccctgg ctttccggtt cgaggaccag tctttcacgg
    11281 aaagatttgg cgccctcgat gcccctgaaa taatttttat aatgtctgcg catttcgagc
    11341 accccaagtt tggggccttt ccatcggacg gaaaaatcaa gatgggcctt taccgcttcg
    11401 atccgttcat ccaggcttgg gggatcgagg atttcaccgg tgcgcaggta atgtttgatc
    11461 tctctgaaga tccagggatg gccaatgctg gccctgccga tcataatgcc atccgggcca
    11521 tagcgttctt tgtattcttt ggctttttgc gggctgtcga tatcgccgtt tccaaaaacg
    11581 ggaataaaaa tggaaggatc atttttaatc cttgcaatat gggtccagtc ggcttccccc
    11641 ttgtacatct gtgaacgggt gcgggcgtgg atgctgatcg cctggatgcc ggtatcctgc
    11701 aggcgcctga ccacttcatc gattttgatg gtattggcgt cccaacccag cctggttttg
    11761 accgtgacgg gcaggcgggt gttttctacc acggccttgg tgagggctac cattttcggg
    11821 atatccttca ggatgccggc ccctgcgtcc cggcaaacca cattgtgtac cggacaaccg
    11881 taattgatat ccaggatttc cggcccggct tcctccacca gcggaatggc ccgcagcatg
    11941 gaatccatct cgcctccgaa gatctggatg ccgatgggcc tttcctccgg ataaatatcc
    12001 agtttctgca agcttttggc cgcatcgcgg atcaggcctt ccaccgagat aaattcagta
    12061 tagagcaggt cgcatccctg cattttgcaa agagcccgga aaggcgggtc gctgacatcc
    12121 tccatgggag caagcaggat gggaaagtct cccagttcga tgtccgcgat tttgaccatt
    12181 tcgaccggca aagatacgat ccgtgaggtt cttataccac aaatcaaata tttcaaacta
    12241 tattggtcgg taaatcattt gagctcaatg gaaatgctgg tcattgtctg cttcatagtg
    12301 ggctaccttc tgatcgtatt tgaacatcct ttaaaacttg ataaaaccat acccgccctt
    12361 ttaatgggta tcctctgctg gacattcata tccatcgggg gattgaatgt atttggggcg
    12421 gaggaggccg ggcaccacga tgtaaacaat gtggttctcc atcatttgtc ctccatcgcc
    12481 gcgatcctga tcttcctgat aggggccatg accatcgtcg aactggtgga cctgcacaaa
    12541 ggttttgcgg tcatcaccaa ccggatccgc accacggata aaagaaaatt attgtggatc
    12601 atctcctggc ttgctttttt tctttcctcg gtattggata atctcacaac taccattgtg
    12661 ttcgtatccc tcctcagaaa attgatccgg gaccgcaatg aacggatctg gtacgtctcc
    12721 tttgtgatca tcgcggccaa tgccgggggc gcctggactc cgatcgggga tgtgacgacc
    12781 accatgcttt ggattgccgg taaggtcagc acttatctgt tgatcgtcaa cctggccatc
    12841 ccttccgtca tttgcctggc cctccctttg tttatcgcca gttttttgaa agtattccag
    12901 ggacagatca gcagcatgca cagtgctgat tccagggacc tcgaacgtga aaaactgctg
    12961 agcagcaaga ccatgctgat cgccggtgtc ggcgcgctca tctttgtacc gatattcaaa
    13021 accgtcaccc acctgccgcc ctggatgggc atgatgctgg gactgtccat cgtatggctg
    13081 atctccgaat acatccatcc ggaagaggat ttttcggaag accggaggca taaatacacc
    13141 gtacgccatg ccctatccag gatcgaatta tcctccatct tgtttttctt agggatcctg
    13201 atggccatat ccgcactgga atccctgggg aaactggcgc aactcgcagc atacatggac
    13261 cgggtgatcc ccaatcagga cgtggtggcg accgtgatcg gcattatgtc ggcggtcatc
    13321 gacaacgtcc cattggtagc cgccacgatg gggatgtacg agctgcccat ggatgccaag
    13381 atgtggcatt ttattgcctt cagcgcgggt acgggaggga gcatgctcat catcggttca
    13441 gcagcgggag tcgctgccat gggtatggaa aaaatcgatt ttatctggta tctcaaacgc
    13501 attgcctggc tggcagcgat agggtttttt gcgggatgtt tggctaccag cctgatccat
    13561 ctgatcctgc acgaagctta aaaaaacagt ctcttgtttt ttagtatatt gcggcctacc
    13621 ggatggttaa ccccaaaccg gtcaaccaca tgatataatt ttatgaatga atggaatggc
    13681 tgatcatcgt tagttttgtc ataggatacc tgctgatcgt tttcgagcat cctctcaaac
    13741 tggacaaggc cattcccgcc ctgtttatgg gcatctttat ctggtctgtc atcgcgctga
    13801 accatatgcc cctgatcgat gtgtcggggg atcacggcac gacggaggcg gtcgcccatc
    13861 acctcggaaa aatcacggaa atcttattgt tcctgctggg tgcaatgacc attgtagagg
    13921 tggtggacgc acaccatggc ttttcggtga tcaccaacag gatcaggacc aaaaacaaaa
    13981 cccatttgct gtggattatt tccgctttgg cttttgtgct ctcatccatc ctggacaacc
    14041 tgaccaccac cattgtcctg gtttctcttc tccggaaact gatttaccgc agggaggaac
    14101 ggatctggta cgtcgctttt gtggtcatcg cagccaatgc cgggggagcc tggtccccga
    14161 tcggggatgt aaccaccacc atgttgtgga tagcaggcaa agtcagttcg gtccagctca
    14221 taattaatct tatcgtgcct tcgatcctgt gcatggcgat ccccctgatc gtggccagcc
    14281 ggatgaaaat attcaaaggc aacctggaag cggcggaaga cgatgaaggg tattattcca
    14341 gcaaatccag tggaattcaa agttcaaaga tcatgctcat ggtaggggtt ggcgccctcg
    14401 tatttgtccc tatatttaaa tcggtcacgc acctgccacc ctggatgggg gtcatgtttg
    14461 gattgggcat tgtctggctg gtgacggaat ttgtggagcc ggaactcgac ctggatgaac
    14521 aagaccggat gaaatatacc ctccggcatg ccctgggccg ggtcgaattg tcgagcattc
    14581 tttttttcct cggcatttta ttggcgatca gtggcctgga atcccttggg attctcaaag
    14641 acctggcggt gaaaatggat gccatcatcc cttcccagga cttggtggcc accctcatcg
    14701 ggatcctttc cgcgatcatt gacaacgtgc cgctcgtggc tgcagccatc ggcatgtacg
    14761 aggtccccct ggatcatgaa ctctggcatt ttatcgcgtt cagcgccgga actggaggca
    14821 gcatgctggt catcggttcg gcagccggcg ttgccgcgat ggggatggaa aaaatcgatt
    14881 tcatctggta cctgaagaaa atcagcggcc tcgcggccct gggctttctg ggcggttgta
    14941 tttacctggc cctggttaat tacctccgcg cggcttaact ttgcgccccg aaactaaatt
    15001 atttttaatg ctcagattca acagaaccca aagcggcctg ctcagcctgg tcgccctgat
    15061 catccttttt tcctgtaaac ccagcgccaa acttcaggtc cttcagccgg ccgacctggt
    15121 gatcccggaa cacatccgga aaattgtgct catcgaccgg agcaagcctg ccggcggctt
    15181 ctggtcaaat gtagagagtg tgctcaccgg cgaggagtac cagcaggaca aacaaggccg
    15241 gcgccgcgcc atggaaggcc tcagtgaatt gctgcagaaa acgccccgtt ttgccaccat
    15301 ctattccaat gtggaaatga ccggctcaaa aggaggccgt gaatttatga ccccgctctc
    15361 ctggagggaa gtggaggaga tttgtaataa attcggaggg gatgccctgg tcgccatcga
    15421 gatgtttgat tccgatatcg aatcctccac taccagccgc agtgtaaaag aaaaggacaa
    15481 ggagggcaaa gaaatcaccc gcgtggtttt cgacggacgc cgccgcggca cggtcaagct
    15541 gggttggagg ttttatgatc ccaaagaccg gaccattgtg gacgaatacc gggataacga
    15601 taccgaggaa gtgaccagca gcggggctgc aagccaacag gccgcgacga ataacctgag
    15661 gaagtcctat gaactcgtta gggatatcgc ttttgacctg ggaggaaagt acggcaaacg
    15721 gatcgccccg gtctggatca ccctttcccg cacctattat aaaaacatca aaggggccaa
    15781 cgaagataag tttgaacagg cggcccgcta tgcggatgcg ggagaatggg ccaaggccgc
    15841 agaaatatgg gaaaaagtgg catcggtccg gtccaatccg gatgcggcgg gcaaggccac
    15901 ctacaacctc gccgtagccg cggaagtcaa cgggcaactc aataccgccc tgcaatatgc
    15961 ccgggatgct tacacccagt tcagtgataa aaaagccaaa aattatatcc ggctcctcga
    16021 acagaggatc caggacctgg aaaccattca gcggcaaatg cacgaaaaaa agacgagctg
    16081 aaccgggggc ttttgagggt aaggttcggg aggaattctg ctgagtcctc aaactgtgta
    16141 aataggttac caccgcgata acaggttttt gaaggatcgt tacttgttgt tttatgtggt
    16201 ctgcttgttg ggcttctccg aagcctgatt ccctctgttg ttaagtgtta agctcgttcg
    16261 gcatctccga tgctgagaaa tgccaattat ttcttggtcg ttgtgccctt tgtgggcctg
    16321 cgcctgcttt cccacaacgg cacagcgaag cgaagggcac gaaggatttt tttgtgtgct
    16381 ttgtggcctt cgtgggagct agctttcccg ttaataattt cccataacgg cacagcgaag
    16441 cgaaggcacg aagcattttt ttgtgttctt cgtgcccttt gtgggagtcc tgcagggagg
    16501 ggcgaagccc cgacctcccg ttgcgcggct gaccaagatg attcccacaa cgacaccacg
    16561 tttttcataa agaatttgtg ttcttcgtgg cctttgcggg agaccggcag ggaggggcga
    16621 agcccccacc tcccgttgcg cggctgacca agatgatccc acaacgactc cacgtttttc
    16681 ataaatactt tgtgttcttc gtgccctttg tgggagtcct gcagggaggg gcgaagcccc
    16741 gacctcccgt tgcgcgactg accaagatga ttcccacaac gacaccacgc ttttcataaa
    16801 tactttgtgt tcttcgtgcc ctttgtggga gaccagcagg gaggggcgaa gccccgacca
    16861 ctcgttgcgc cgctgaccaa gatgattccc actgcgaccc cactttttta ataaatactt
    16921 tgtgttcttc gtgccctttg tgggaatcct gcagggaggg gcgaagcccc gacctcccgt
    16981 tgcgcggctg accaagatga ttcccacaac gactccacgc tttgcataaa taatttgtgt
    17041 tcttcgtggc ctttgtggga gaccggcagg gaggggcgaa gccccgaccc cccttgcg
//
LOCUS       DAOQXT010000103        16944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3802_length_16944_cov_6.760199, whole genome shotgun sequence.
ACCESSION   DAOQXT010000103 DAOQXT010000000
VERSION     DAOQXT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16944
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3802_length_16944_cov_6.760199"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1420)
                     /locus_tag="PKM27_15400"
     CDS             complement(377..1420)
                     /locus_tag="PKM27_15400"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08704.1"
                     /translation="MKPVYIALCSFGMSGTVFHAPFLSRHPGFKLYAVWERSKNLSAE
                     AYPGTLVYRTLEDLLADPDVELVIVNTPSYTHFNYARQALSAGKHVVVEKPFTATAGE
                     ARELVDMARQRGLKLSVFQNRRWDSDFKTVKGIVEEGVLGEILEAEFHYDRYNLSLSP
                     KAHKETPGPAVGCLYDLGPHLIDQALHLFGMPEAVFADLMITRPGSKVDDYFEILLYY
                     PARRVRLKSGYQVREPVPSFVLHGSKGSFLKSRADVQETHLQAGRSPAEADWGMEPES
                     AWGLLHTDIEGTVIYKRIPSLLGNYMEYYEGVYQSLRNHAPMPVSGEDGWNTIKIIEA
                     ARASHASRSVVGV"
     gene            1556..2719
                     /locus_tag="PKM27_15405"
     CDS             1556..2719
                     /locus_tag="PKM27_15405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR08705.1"
                     /translation="MITPEKYRFFDRMLEGVQLISRDYHYLYVNEAIARQGKSTIGAL
                     MGHTMMEVYPGIEHTEVFGRIEACMNNQTPDHLISEFVFPDGSKGWFDIRMEPVEEGV
                     FIFTFDITEQKKLEGELKKLNANLSKQVQEKTRELSEFLDRERELNQMKSRFVSMASH
                     ECRTPLSTILSSISLIEKYELTEQQPNRVKHIRRIRHSVLSLTALLDEFLNLEKLETG
                     NMSVHFTEVEIKPFISEILGELEGIFKFGQRILYSHQGHSNAILEKQIFRNVLLNLLS
                     NAIKYSDKDIELQTLVHDDHVMVQVRDQGIGIPEEEQKSLFDRFFRATNALNVQGTGL
                     GLNIVKRYLELVQGSIDFTSKENEGTTFIVYLSQKNLMEEPAEAKPHKTIGRL"
     gene            complement(2778..3719)
                     /locus_tag="PKM27_15410"
     CDS             complement(2778..3719)
                     /locus_tag="PKM27_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08706.1"
                     /translation="MIKLLPLFLILLAIASCKSDKRPEALPDKEPLGIEWSKDVPAKH
                     DIVNGRAVTYKDDIYVLAGVAGRFMKYHPETYAWTDLAGLPAPRTEAAMILWNDKIIV
                     AGGVDDSSHLMSRVDYYDLNDQVWRMFASLPQGRARFQLNIYDSILYANGGACGANER
                     TYSPCTEVVSYNDSTMQWEILTNIPSSRFGHVSVSNGPELYMIRGYGLVPNMGTYYLN
                     HLDKQVDTRPAMPNHRGNFGAVLVGDYILTFGGKTREMNSPMERYSIKEKKWEPLPSC
                     PFYTDRFAYTRLKNTIYVFGGSQAPLQVWKGKIVFKE"
     gene            3805..4539
                     /locus_tag="PKM27_15415"
     CDS             3805..4539
                     /locus_tag="PKM27_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HNR08707.1"
                     /translation="MSALIQTEGIVVRTIKYGETSVITEVLTRKLGSQSFIMGGVTAG
                     NARSGLFQIMNQLEVVVYYKENRTLHRIKEARFLKTYQALSFEIHRSAIGIFILEMVR
                     KCLRGSVQDEALYQWVKDCLDLADQRTTPLPWLPVYFALGFTRFLGIQPLKCADPDHR
                     VFDLREGLFLNRAPHHPDYLEGAATALLSRVLECEDILELAEINSTTRSTRKALLASI
                     LDYYRMHLDHFQGLESPQILEEILTA"
     gene            4575..7010
                     /locus_tag="PKM27_15420"
     CDS             4575..7010
                     /locus_tag="PKM27_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNR08708.1"
                     /translation="MNIPFGEEIRSFVKAAFEPASIWEKGLRICALFFVAGLFSFFFL
                     SLLVRIGLFGSIPGTSELRLIKNPVSSVLLDDENRLLGKYFIENRTNVHYKAIAPHVI
                     QALVATEDERFYRHHGIDLRSWARVLVKSFLLMDQSSGGGSTLSQQLAKNLYPRKDLW
                     LLTTPINKVREMYIARRLEKIYNKEEILTWYLNTVPFGNGIYGIEVACKQLFNTEPDK
                     IKIQDAAVLVGMLKATGIYSPLRNEERASDRRNVVLKQMVKNGFLAQAEYDSLAQLPI
                     KLKYTREGHTEGVGTYIREQIRLDLNKLLKDFLKEDGKPYNLYTDGLKIYTTLDVHMQ
                     RLAEAAVRERMARLQADFDNHWKKREPWGQTSLLSDQVAKTERYLYMKKNGYSAAEIE
                     EAFNQKTAMTLFTYKGPVDTLLSPLDSLKYYLRMLNAGFLALDHSSGKIKAWVGGVDY
                     ATFKYDHVKSKRQVGSTFKPIVMAAGLQQDFTPCDYFSNDRLVYAQYDGWSPENTDGK
                     YGGFYSMEGTLVHSVNCAAVDAIIQTGTGPVIELAGKLGIHSELPQVPSLALGTADIS
                     LMEMVQAFGVFANQGLRAEPYYLKRIENNKGEVLVDFEKDRGLPTEVLSPLQAELMVQ
                     MLKSVAENGTAKGLKTMYSIGTEIGAKTGTSQNQSDGWLIAITPKLALGAWVGGEMPQ
                     IRFRSLSLGQGSYMALPICGSFLHKVNASKVLKKYQGGVFPPLSDEAMSRLGCPYFIE
                     NEGDQLELIALGDSLQPGPAFVNSPPDTVVDVSQRRRARDRESRGLGAFLERLFGKKD
                     RKEKRDSLENSGH"
     gene            complement(7121..8383)
                     /locus_tag="PKM27_15425"
     CDS             complement(7121..8383)
                     /locus_tag="PKM27_15425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR08709.1"
                     /translation="MLSTTKTDALRPSYSFTGKQLLMVAVAALGYFVDVYDLLIFSSE
                     RIESLQAIGVAREDMKDVGIMILNFQMAGLIIGGFLFGIMADKYGRLKVLFASILLYS
                     LANIGNAFVTGIPGFIVARFLAGIGLAGELGVALSWISESLDRKQRTIATMIVSAIGL
                     FGGIAAALVSANFDWQTSYIVGGVLGLLLLGLRISVRESKMFIKNSSKKAIRGDLVEL
                     FTNNKQLYKYTLCVLAGAPAVVFISLFITLTPEFCRAFGITEEVSVSNAIMYYLISLA
                     ISDILCGYLSKLMQQRKGPLLIYAGLQIIATVYFLLIPPATESGYYVRCVILGFSVGY
                     WGVLVTNSVEQFGTNIRATVATSTPNLIRGITIPATLIFSALVKPLGLVTSGAVIAFS
                     LIGISIISILLLKDKFENNLNYTEIFED"
     gene            complement(8520..9203)
                     /locus_tag="PKM27_15430"
     CDS             complement(8520..9203)
                     /locus_tag="PKM27_15430"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNR08710.1"
                     /translation="MKFLQAPWKISGMAGVLFVALSFIASGMNKMPPRYNQEISTFVS
                     WFSENGDRYRFGHFLAGLAFLLFYFPFFSGFCEVLRKAEGEPSIWTRVTWAGAIMSPA
                     AGTVAGAFIMGIVLLNGNASPELSQFGMAGNFYAYTVSGAYGGIAMIGSSVIILKTNI
                     FKRWLGWAGLVIGSTAIISIGTLVENNPQGLFATINGFAWLAYFLWIGVLSIELIKRK
                     ETRGGKEAD"
     gene            complement(9317..9688)
                     /locus_tag="PKM27_15435"
     CDS             complement(9317..9688)
                     /locus_tag="PKM27_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNR08711.1"
                     /translation="MKPLISNKDYDMLKSVIRQWSPVSKTKEVHLLEQELAKVKVVND
                     QELTPDVIRLNSYFEVAEESSNQLLNFTLVLPGHADFKMKKISVLSPLGIALIGFKEG
                     MTIDCALPAGKKIFKILKVLN"
     gene            complement(10212..10601)
                     /locus_tag="PKM27_15440"
     CDS             complement(10212..10601)
                     /locus_tag="PKM27_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08712.1"
                     /translation="MANPENSTHFAPELHIPNGTFNIDFYLRFGATENFCFRNEDGSI
                     HVAELEFDGCVFHLHETMRGALEPVSAKGVTAVIGLFVENVHDVFNRAIAAGATEIDP
                     VTDHDYGYRQGMFKDPFGHYWQIQKRI"
     gene            complement(10635..11216)
                     /locus_tag="PKM27_15445"
     CDS             complement(10635..11216)
                     /locus_tag="PKM27_15445"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNR08713.1"
                     /translation="MRKIISFMHLSLDGFVAGPRGELNWVKVDEEIFDFVGKRISQTD
                     TALYGRVTYQMMENYWPSAGDKPGATRHDQEHSKWYNTAHKVVLSNTLREAELTNTEI
                     ISDHLSDRINEIKQSGGSGSKEILLFGSPTATHALIQRGLIDGYWLFVNPVILGRGIP
                     LFVDIKEKIKLKLLTTRPFASGVTELNYIVDRE"
     assembly_gap    11284..11383
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11470..11592)
                     /locus_tag="PKM27_15450"
     CDS             complement(11470..11592)
                     /locus_tag="PKM27_15450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR08714.1"
                     /translation="MSDLERALDYYCGLLGFEFTTRYGIDAVFISAGGVHHHSA"
     gene            complement(11661..12326)
                     /locus_tag="PKM27_15455"
     CDS             complement(11661..12326)
                     /locus_tag="PKM27_15455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNR08715.1"
                     /translation="MKEDFLANENKELNRFLVFSASMRKDSLNTHLARLAKETIEKLG
                     GQVDFASMAEFDCPSYHGDHEKLGGIPEGATAFNQRILANDALVISSPEYNGSMPGLL
                     KNAIDWVSRFRPQPFNEKQILLMSASPSMVGGNRGVLALRIPLEHLGGRVFPNMFSLA
                     MAHKAFTPDWKIGDETLAKRFEDNMVSFIQLVEAQKHYPCIKKAWVEFLGEKTDALTE
                     RAE"
     gene            complement(12510..12956)
                     /locus_tag="PKM27_15460"
     CDS             complement(12510..12956)
                     /locus_tag="PKM27_15460"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNR08716.1"
                     /translation="MNYLMQLIPLLKQKGLLWGAMALFITGVNSPTDPAAALAEIDKS
                     AVNPHNNMAIKAIIELQAHPGKRDELKAFIENLVATHGPGGPGFIRSTRYEVLGNPDK
                     LIEIADWDSAEAREAHMKAAAASGIYAPLKDLLAEPFRVTIIKQME"
     gene            complement(13229..13762)
                     /locus_tag="PKM27_15465"
     CDS             complement(13229..13762)
                     /locus_tag="PKM27_15465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNR08717.1"
                     /translation="MKNIFVINGSASEHSSNQKLINIVEERTKDFFKLTVFNDLKTLP
                     HFDPDRSVNNTPEVILDFRNRIEAAEGILICTPEYVFSIPSGLKNAIEWCISTTVFSG
                     KPTGLITASASGEKGHEELQLIMKTAMANFTMDTTLLIQGVKGKIDEQGRLTDSHTKD
                     NLDKFLLAFKNLVKNAL"
     gene            complement(14472..15869)
                     /locus_tag="PKM27_15470"
     CDS             complement(14472..15869)
                     /locus_tag="PKM27_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR08718.1"
                     /translation="MPSLKETYKLVVQALKGEHTDYTAGSMKRAIVLLAIPMILELSL
                     ESVFAVVDMFFVGKLGPGAIATVGLTEAVVTLVYSAGIGLSMAATAMIARRIGEKNPE
                     AANHAAVQSLIISLVATLVISILGIVYAPHILQFMGATPEVVRDGTPFTRIMLGGSVV
                     IILLFLINGIFRGAGDAAMAMRSLWIASGLNIVLCPLLIYGLGTWEGYGLKGAAIATT
                     LGRGAGVLYQCYHLFNGKGEIRIRLSQFKADWKVIRGLLDIAWPATFQFIIASGSWIV
                     LTRLVAETGGTEASAGYQVAIRNVVFFILPAWGLSNAAATLVGQNLGAGQPDRAEKSV
                     LLTARYNIYFMALVTLLFTAIPGPIIRIFSQEPEVVKTGIDALRIIGTGYIFYGIGMV
                     MMQAFNGAGDTRTPTWINFVGFWCFQIPLAYVLSGVFHLGTDGVFIAIPAAETAIALA
                     CYYFFRKGKWKTVKV"
     gene            complement(15887..16750)
                     /locus_tag="PKM27_15475"
     CDS             complement(15887..16750)
                     /locus_tag="PKM27_15475"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNR08719.1"
                     /translation="MKKIIVILLWCVLGGLTAFGQETHLGLRIGLNYATMNGTLEENA
                     GFGTSPGFHVGPVFSYGFSDIMGLRGELIFSQKGSKFNYNGPSYFTIKKNQEYIPTSG
                     VRVMNSVINNNYLDLPLMFYTLFFERLDVAVGAYASALIGSTGRGDLEYTGKRDNSNA
                     QVGPIRFLLDQNYFKDKAGSVASSVTQTIRLEGDQIVLPERIGGYYEYRTKPDGNRYN
                     SLDYGLTGSVHYRLSRGLYAGIRYQVGMADVSNDDFEVSYKSLNPSKEFILIPEKHTN
                     RTLQLSLMLQL"
BASE COUNT         4225 a         4354 c         4087 g         4178 t
ORIGIN      
        1 gaccaggtct tccgcccttt tctccatggt cagatccgga ttttgaaaag ggaagtcaag
       61 gtttctttga gtttggtttt tgcaggaaaa caatactata aagacagatg cgaggatcgc
      121 cgggtggaac cattttttca ttagcaggat tttggaggaa tcagtcggat aggtgggtta
      181 aagataacaa gtaatttgac ctaacctatt tgacctaatc cctgatattt gcgaagatcc
      241 gatcctgcaa gggtcagtca gactgggtcg tacatttcca agactgtccg ctacgggtta
      301 gttattttct gtttgtcagt ttatcagcta tatcaacttg ctaacttgct aacttaccaa
      361 cttgctaact tactaactaa accccgacta cactgcggga agcatggctg gccctcgcgg
      421 cttcaatgat cttgatggta ttccatccat cctctccgga tacgggcatg ggagcatgat
      481 tccgcaggct ttgatagacc ccttcataat attccatata atttccaagc aaggagggaa
      541 tccttttgta aatgaccgtt ccttcaatat ccgtgtgcaa taatccccag gccgattccg
      601 gttccatccc ccaatccgct tcggcaggtg atcttcccgc ctgcaagtgg gtttcctgca
      661 catccgccct ggatttcagg aaggaccctt tggaaccatg gaggacaaaa gaggggacgg
      721 gttcccggac ctggtagccc gatttcaaac gaactctccg ggccgggtaa taaagcagga
      781 tctcgaaata atcatccact ttcgaaccgg gccgggtgat catcaggtca gcgaagacgg
      841 cctccggcat tccgaacaga tgaagggcct gatcgatcag gtgggggccc aggtcataca
      901 ggcatcccac cgccggcccg ggcgtttcct tatgcgcttt tggactcagg ctcagattgt
      961 accggtcgta atgaaactcg gcttcaagga tctctccaag caccccctct tccacaatgc
     1021 cttttacggt cttaaaatca ctatcccagc gccggttttg aaacaccgac aatttaagcc
     1081 cacgttgcct ggccatgtcc accagttccc tggcttctcc ggccgtagca gtgaatggtt
     1141 tttcaaccac cacgtgtttg ccggcggata aagcctggcg ggcatagttg aaatgagtat
     1201 aactgggcgt gttcacaatg accagctcca catcgggatc cgccagcagg tcctccaggg
     1261 tccggtaaac cagggtcccg ggataggctt ccgcactcag gtttttcgat ctttcccaga
     1321 cggcatagag cttaaatccc gggtgccggc tgagaaaggg cgcatggaaa accgtaccac
     1381 tcatgccaaa cgagcagagg gctatataaa caggcttcat gtcaggaaga taaaaaaatg
     1441 aaatcaatta caccggcttg cctgtccggc ccgcttgttg caattgcggc atttaaaaag
     1501 taaatttaaa ctcccgatat ccctgtgagt tatatctgtt agcctttcag tccttatgat
     1561 tactcccgag aaatatagat tttttgaccg catgctcgag ggggtccagc tcatcagcag
     1621 ggactatcac tatctctatg tcaatgaagc catagcccgg cagggtaaat ccaccattgg
     1681 agcgctcatg ggtcatacca tgatggaagt ctatcccggc atcgaacaca cggaggtctt
     1741 tggacggata gaagcctgca tgaacaacca gaccccggac cacctcatca gtgaatttgt
     1801 ttttcccgac gggtccaaag gatggtttga tatccggatg gaaccggtgg aagaaggcgt
     1861 tttcattttc actttcgata tcacggagca gaaaaaactg gaaggtgaat taaaaaaact
     1921 gaatgccaac ctttccaaac aagtccagga gaaaacccgg gaattgtccg aatttctgga
     1981 ccgggaaagg gaattgaatc agatgaagtc ccggtttgtc tccatggcct cccacgagtg
     2041 ccggaccccc ctcagtacta tcctgtccag catatccctc atcgaaaaat acgagctcac
     2101 cgaacaacag cccaaccggg tcaaacacat ccgccgcatc cggcattcgg tgctcagcct
     2161 cacggccctc ctggatgaat tcctgaatct tgaaaaactg gagaccggaa acatgagcgt
     2221 ccatttcacc gaggtggaga ttaagccttt tatttccgaa atcctggggg aactcgaagg
     2281 catctttaaa ttcgggcagc ggatcctgta ttcccaccag ggccattcca atgccatcct
     2341 tgaaaaacaa attttcagaa atgtgctcct gaacctcctt tccaatgcca tcaaatattc
     2401 cgataaggac atcgaactgc agacccttgt ccacgatgac catgtgatgg tccaggttcg
     2461 cgatcagggc attggtatcc cggaagaaga acagaaaagt ttattcgacc ggtttttccg
     2521 cgcgaccaat gccctgaatg tgcagggcac cggccttggc ctcaatatcg tcaaacgcta
     2581 cctcgaactg gtccagggga gcatcgattt taccagcaaa gaaaatgaag gcacaacctt
     2641 tatcgtctac ctgtcccaga aaaacctgat ggaagaaccc gcggaggcca aaccccataa
     2701 aactataggg cgcctctaaa ttccatccat tgaatgcccg gggattcccg gttcaaggcc
     2761 ttgaaaggtt ttcaggacta ttccttaaat acaattttcc ctttccacac ttgcaggggg
     2821 gcttgcgatc cgccaaacac atagatggtg tttttcaagc gggtataggc aaaccggtcc
     2881 gtataaaagg gacaggatgg caagggctcc catttttttt ctttgatgga ataccgctcc
     2941 atcggggaat tcatttccct ggtttttccg ccaaaagtca aaatataatc gccgaccagg
     3001 accgccccga aatttccccg gtgatttggc atcgcaggcc gggtatcgac ctgcttgtcc
     3061 aaatggttca ggtaataggt gcccatattg ggtaccaggc catagccgcg gatcatatag
     3121 agttccgggc cgtttgacac cgagacatgc ccaaaacggg aagaggggat attggtcagg
     3181 atttcccatt gcatcgtgga atcgttgtag gaaacaacct ccgtgcaggg gctgtaggtc
     3241 ctttcatttg ccccgcaggc ccccccattg gcatataaaa tggaatcgta aatattcagt
     3301 tgaaaccggg cgcgcccctg gggcaaggag gcaaacatcc tccatacctg gtcattgagg
     3361 tcataataat ccacgcggct catcagatgg ctggaatcgt ccacccctcc agccacgatg
     3421 atcttgtcgt tccacaggat catggccgcc tcggtccggg gggcggggag gccggcaagg
     3481 tcggtccagg cataggtttc cggatggtac ttcataaagc gccccgccac ccctgcgagc
     3541 acataaatat catccttata agtcaccgcc ctgccgttga cgatgtcgtg tttggccggc
     3601 acgtcctttg accattcgat gcccagcggc tctttgtccg gaagggcttc cggcctcttg
     3661 tccgatttgc aggaagcgat ggccaggagg attaaaaata aaggcaggag tttgatcatg
     3721 cgcgcaaagg taaaaagttc aaaccggatt aatattataa aaaaatataa tatgattttg
     3781 ggccgcttat tgtaatttta ccgggtgtca gccttaatcc aaaccgaagg gatcgtcgtc
     3841 cggaccatta aatacggaga gaccagcgtc atcacggaag tccttacccg aaaactgggc
     3901 agccaatcct ttattatggg cggagtcacc gcgggcaatg ctcggtcagg ccttttccaa
     3961 atcatgaacc agctcgaagt ggtggtttat tataaggaaa accggacgct tcaccggatc
     4021 aaggaagccc ggtttttaaa gacctaccag gccttgtcct tcgaaatcca tcgaagcgcc
     4081 attggcattt tcatcctgga aatggtgagg aaatgcctgc ggggttcggt ccaggatgaa
     4141 gccttgtatc aatgggttaa agactgcctg gacctggcgg accaaaggac caccccgctt
     4201 ccctggttgc cggtctattt cgcgctcggt tttacgcgtt tcctgggtat ccagcccttg
     4261 aaatgcgccg acccggacca ccgggtattt gacctgcggg agggcctttt tttgaatagg
     4321 gccccgcacc acccggacta ccttgaaggg gcggccacgg cccttttgag cagggtcctg
     4381 gaatgtgagg acatcctgga actggcggag atcaactcaa ccacccggtc cacccggaag
     4441 gctctactgg caagtatatt ggattattac cggatgcacc tggatcattt ccagggactg
     4501 gagtccccgc agatcctgga ggaaatcctg accgcctgaa atagaacctg tttttttagc
     4561 gtttatagat atccatgaat atacctttcg gagaggagat ccgatctttt gtaaaagccg
     4621 cctttgaacc ggcctcgatc tgggaaaagg gactgaggat ctgcgccttg ttttttgtcg
     4681 ccggcctgtt ttcctttttt tttctcagcc tcctggtccg gatcggctta ttcggttcga
     4741 tccccgggac cagtgaactc cggctgatca agaatcccgt ttcctccgta ttgcttgatg
     4801 atgaaaaccg cctgctggga aaatatttca tcgaaaacag gaccaatgtc cattacaaag
     4861 ccatcgcccc ccatgtgatc caggcccttg tggccacgga agacgaacgt ttttacaggc
     4921 atcacggcat cgacctccgg agctgggccc gggtattggt taaatccttc ctgctgatgg
     4981 accaatcctc gggaggagga agcaccctca gccagcaact ggctaaaaac ctgtatcccc
     5041 gcaaggacct ctggctgctc accaccccca tcaacaaagt ccgggaaatg tatatcgccc
     5101 ggaggttgga aaagatctac aacaaagagg aaatcctgac ctggtacctc aatacggtgc
     5161 ctttcgggaa cggcatctac ggcattgaag tggcctgcaa acaattgttc aataccgaac
     5221 cggacaagat caagatccag gacgcggccg tcctggtggg catgctcaag gcgaccggca
     5281 tttacagccc gctccggaat gaagaaaggg cttccgaccg gaggaacgtg gttttaaaac
     5341 aaatggtcaa aaacggtttc ctggcccagg ctgaatacga ttcgcttgct caactgccta
     5401 tcaagctgaa gtatacccgg gaagggcata cggagggggt gggcacctat atccgggagc
     5461 aaatccgcct ggacctcaac aaattgctaa aggatttcct caaggaagac ggcaaaccct
     5521 ataatctcta taccgatggc ctgaaaatat ataccaccct cgatgtgcac atgcagcgtc
     5581 tcgcggaagc cgcggtccgc gaacgcatgg ccaggctcca ggcagatttt gacaaccatt
     5641 ggaaaaaaag ggaaccctgg ggccagacct ccctgctgtc cgatcaggtc gcaaaaacgg
     5701 agcgttacct ctacatgaaa aaaaacggct acagcgctgc ggaaatcgag gaagctttta
     5761 atcaaaaaac ggccatgacc ctttttacct ataaagggcc ggtggatacc ctgctttccc
     5821 ccctggattc actcaaatat tatctccgca tgctcaatgc cggttttctg gccctcgatc
     5881 acagcagcgg taaaatcaaa gcctgggtgg ggggcgtgga ctacgccacc ttcaaatatg
     5941 atcacgtaaa atccaaaagg caggtgggct ccactttcaa acctatcgta atggccgccg
     6001 ggctccagca ggacttcaca ccctgtgatt atttttcaaa cgaccgcctc gtctatgccc
     6061 aatacgacgg ctggtccccg gaaaacaccg acggcaaata cgggggcttt tattccatgg
     6121 aaggcaccct ggtgcattcg gtcaattgcg cagccgtgga tgccatcatc cagaccggga
     6181 ccggtccggt gatcgaactg gccggaaaac tagggatcca ttccgagctg ccccaggtgc
     6241 cttccctggc tcttgggaca gcggacatct ccttaatgga aatggttcag gcttttggag
     6301 tctttgccaa ccagggtctg cgggctgagc catattacct caagcggatc gaaaacaaca
     6361 agggcgaagt cctggtggat ttcgaaaaag accgcggact tcccacagaa gtgctgagcc
     6421 ctcttcaggc agaactcatg gtccaaatgc tgaaatccgt ggcggaaaac ggaacggcca
     6481 aagggctcaa aaccatgtat tccatcggca ccgaaatcgg ggcaaagacc gggaccagtc
     6541 agaaccagtc ggatggctgg ctgatcgcca tcacgcccaa actggcgctt ggcgcctggg
     6601 tcggcgggga aatgcctcaa atccgtttcc gctcactttc cctggggcag ggatcctata
     6661 tggcgcttcc catttgcggc agttttctac acaaggtaaa cgccagcaaa gtgctgaaaa
     6721 aataccaggg aggcgttttc cctcccttat ccgatgaggc catgagccgt ttgggctgtc
     6781 cctactttat tgaaaacgaa ggggaccaac ttgaattgat tgccctgggc gatagcctgc
     6841 aaccgggacc ggcctttgta aacagcccgc ccgatacggt ggtagatgtg agccagcggc
     6901 ggagggcgag ggaccgtgaa agccggggac tcggggcttt tttggaaagg ttattcggaa
     6961 aaaaagacag gaaggaaaaa agggactcgc ttgagaattc tggccattga cggatttgcc
     7021 ggaaacttaa ggagatatgc atccctccag atattagtgc aaaatgatag taccagttta
     7081 aatctgcact aattaatttg tctggtcggg gtattattct ctaatcctca aagatctccg
     7141 tgtaattcag gttgttctcg aatttgtcct tgagtaagag gatgctgatg atgctgatgc
     7201 cgatcaggga aaatgcgatg accgctccgc tggtgaccag gcccagtggt ttgacgaggg
     7261 cagaaaagat aagggtcgcc gggatggtga tgccccggat gaggttgggg gtggaggtcg
     7321 ctacggtagc ccggatattg gttccaaact gctcgacgct gttggtgacc aggacacccc
     7381 agtaacccac tgaaaaaccg aggatcacac aacggacata ataacctgac tcggtagctg
     7441 gcgggatcag taaaaaataa acggtggcta tgatctgaag gccggcatag atcagcaggg
     7501 ggcctttgcg ttgttgcatc agtttgctca aatacccgca aagaatgtcg gagatggcca
     7561 gggatatcag ataatacatg atcgcattgc ttaccgaaac ctcttcggta atcccaaagg
     7621 cacggcaaaa ctccggcgta agggttataa aaaggctgat aaacaccacg gccggagctc
     7681 cggccaacac gcacaaggtg tatttataaa gttgtttatt gttcgtgaac agttcaacga
     7741 ggtcgccgcg gatggctttt ttggaagagt tttttataaa cattttgctt tccctgacgc
     7801 tgatccggag gcccagcagg agcagaccca gcactccgcc aacaatatag gaggtctgcc
     7861 aatcaaaatt ggccgagacc agggctgcgg cgatcccacc gaacaggccg atcgcggaca
     7921 cgatcatagt ggcgatggtc ctttgtttcc ggtcaaggct ttcgctgatc cagctcaggg
     7981 ccacgcccag ttctcccgcc aggccgatgc ccgctaaaaa cctggcgacg ataaagccgg
     8041 ggatgccggt gacaaaggca ttgcctatat tggccaggct gtaaaggagg atgctggcaa
     8101 agaggacctt gagccggccg tatttatcgg ccatgatgcc aaagaggaag ccgccgatga
     8161 tcagtccggc catctggaaa ttgaggatca tgatcccgac gtctttcata tcttccctgg
     8221 cgaccccaat cgcctgcagg gactcgatgc gttccgaact gaaaataagc aggtcataaa
     8281 cgtccacgaa atatcccagg gccgctacgg ccaccatcag gagctgtttt ccggtaaaag
     8341 aataggacgg ccggagcgcg tcggtcttgg tggtagaaag cattttggtt ttttggattc
     8401 ggaccgaaaa tacaaatttt aaaaaaacca aatagcatac tcccacccgg gcttgatccc
     8461 cctatccctt acgctccttt gaccttttac ccttcctccc tttctccgaa caagcggttt
     8521 tagtccgcct cctttccgcc tcgggtttcc ttccttttaa tcaattcaat ggacagaacc
     8581 ccgatccaga gaaaataagc caaccatgca aagccgttta ttgtcgcaaa caatccttgt
     8641 ggattattct caaccaatgt tccaatactt ataattgctg ttgaacctat tactagtcca
     8701 gcccagccca accatcgttt aaaaatgttc gtttttaaaa taattacaga tgaaccaatc
     8761 attgcgattc ccccataagc tcccgatacg gtgtaagcat aaaaatttcc agccattcca
     8821 aattgagaca gttcggggga agcattccca tttaaaagaa ctattcccat aataaatgca
     8881 cctgcaacag taccggctgc tggagacatt attgcgccag cccaggtcac tcttgtccat
     8941 atggagggtt ctccttctgc tttcctgagc acttcacaga agcccgaaaa aaatggaaaa
     9001 taaaaaagca gaaaagccaa acctgccaga aaatgcccaa aacgataccg atctccattt
     9061 tctgaaaacc aactcacaaa agtcgaaatt tcctgattgt acctgggagg cattttattc
     9121 attcctgaag caataaaaga taacgcaaca aaaagcaccc cggccattcc gcttattttc
     9181 cagggtgctt gtaaaaattt catttatata ctccttgtgt tgagtttgat tattccaggt
     9241 gatctggacc cttaggttat tctaaaacca aaccctgtgg tcattttctc atactacacc
     9301 ccacaggcgc caaacgctaa tttagcactt tcagaatttt aaatattttc tttcccgccg
     9361 gcaaggcaca atcgatcgtc atcccttcct taaaaccaat aagggctatg cccagtggag
     9421 ataaaacgga tatttttttc attttaaaat ccgcatgacc tggaagcaca agtgtaaaat
     9481 tcagcaactg attggagctt tcctccgcta cttcaaaata ggaattcagg cggatgacat
     9541 ccggagtcaa ctcctggtca ttgaccactt ttactttggc cagttcctgc tccaggaggt
     9601 gcacttcctt cgttttgctg accggtgacc attgacgtat gacggacttg agcatgtcgt
     9661 aatctttatt tgaaattaaa ggtttcatgt tttttgaatt ttaatgaatt cgggcgtttt
     9721 acgatttttg gaataagtaa accgctaaaa ttttttttag tttttattcg tgattccaga
     9781 atataaacag gtaatcgggc atttcctcac tattcaccga atcccggtaa aatcaaccgg
     9841 aaatgaatat gtcctgcctt ctacgccctt ccggaggcat gaaccgagtc gtgaacagca
     9901 tggcttctta cgaaccggga acggccggaa aaaaacctgg aagaaggata tcccctgttt
     9961 ccagtaaaat gaaacagggg cttaagccac gaaggctttg gagtgcataa atcccagggc
    10021 cgtgcctgcg gcgaagggac acatacaaga gaccagcgtc aaggctgacc gctgctccat
    10081 aaatttatat gccggaatac ataaaggcat tttgacttgc cgtattttaa ttgactgcaa
    10141 agttacccgt tgtttttatt aaaagcaatg cctgagcata tgcccgatca gggtatggcc
    10201 ccgttccccg gttagatccg tttctggatc tgccaataat gcccgaaggg gtctttgaac
    10261 attccttgcc ggtaaccgta atcgtggtcg gtgaccgggt cgatttccgt cgctcctgct
    10321 gcaatggccc ggttaaacac atcgtggaca ttttccacaa acaaaccgat gacggcggta
    10381 accccttttg cacttacagg ttccagggcg ccccgcatcg tttcgtgcaa atgaaaaaca
    10441 caaccgtcaa attccaattc agccacatga atacttccgt cctcattccg gaaacaaaaa
    10501 ttttcggttg cgccaaacct gaggtaaaaa tcaatgttga aagtcccatt ggggatatgc
    10561 aattcaggag caaaatgcgt ggagttttcc ggattcgcca tattcggggt ttttgggtgg
    10621 tcagcggttc tttgttactc cctgtccacg atataattca attccgttac gccgctggca
    10681 aagggccgtg tagtcaacag ttttagcttg attttttctt tgatgtccac aaacagggga
    10741 atgccccgtc caagaatgac cggattgaca aacagccagt agccgtcaat caggccccgt
    10801 tgaatcagag cgtgggtcgc ggtcgggctt ccaaaaagca ggatttcttt actcccgctc
    10861 ccgccggatt gttttatttc atttattctg tccgacagat ggtcgctgat gatttctgtg
    10921 tttgtcaact ccgcttccct taaggtgttt gataaaacca ctttgtgcgc agtattatac
    10981 cacttggagt gttcctggtc gtgccgggtc gctcccggct tgtctcccgc ggaaggccag
    11041 taattttcca tcatctggta agtcacccgt ccatataagg ccgtgtccgt ttggcttatc
    11101 cgtttcccca caaaatcaaa aatttcctca tccactttga cccagttgag ttcacccctc
    11161 ggcccagcta caaaaccgtc cagcgaaagg tgcataaatg aaataatttt tctcattccg
    11221 ttctgtttat tgttgctgcg caaagatagt aagattagga atgcatggaa atggaacgga
    11281 tacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnattgttg ctgcgcaaag
    11401 atagtaagat taggaatgca tggaaatgga acggatacgc cggaagggcg ttcttactgt
    11461 accaggtatt caagcactgt gatgatggac cccaccggcg gaaatgaaga ccgcgtcaat
    11521 accgtaccgg gtcgtgaact caaatccaag aaggccgcag taataatcca gggcacgttc
    11581 gaggtccgac acttttagat gtacatggcc gactctggtc tgagccggga ttatgtattt
    11641 gtttccatcc taagaggcat ttattccgct ctctccgtaa gcgcatcggt tttttctccc
    11701 aaaaattcca cccacgcctt tttgatgcag ggataatgtt tctgcgcttc caccagttgg
    11761 ataaaagaca ccatattgtc ctcaaaccgt ttagccagcg tttcatcccc gattttccag
    11821 tccggggtaa atgctttgtg ggccatggcc agggaaaaca tgttcgggaa tacccttccc
    11881 cccaaatgtt caagcggaat gcgcagggca agcacccccc ggttgccgcc caccatggaa
    11941 ggggaagccg acatcaggag gatctgcttt tcattgaagg gctgcggacg aaagcgtgaa
    12001 acccagtcaa tcgcattttt aagcagaccg ggcatggagc cgttgtattc aggcgaagaa
    12061 ataaccaggg catcgttggc gaggatgcgc tggttaaaag cggttgcccc ctccggtatg
    12121 ccgcccagtt tttcgtggtc gccgtggtag gaagggcagt caaactccgc catggatgcg
    12181 aagtctactt gtccgcccag tttttctatc gtttcttttg caagtcttgc aagatgcgta
    12241 ttgagcgagt cttttcgcat cgaggctgaa aacacaagaa accggttcag ctccttgttt
    12301 tcattggcaa gaaaatcctc tttcatataa atcgtttccg tgtttgatat taatcagtaa
    12361 attttagacg aggtgtttgc ttggcatcgt ctatttcgcg tcaagctgct aagacgctat
    12421 atagtatatt tcgtaataac gcattttcga aatgacgctt tatcattgct cttcctttgc
    12481 gccttcgcgt cttggcgaga aaccgatccc tactccattt gttttatgat ggtcaccctg
    12541 aaaggttccg cgagcaagtc tttgagcggc gcataaattc cggaagctgc cgcagctttc
    12601 atatgcgctt ctctcgcttc tgccgaatcc cagtctgcta tttcaatgag tttatcgggg
    12661 ttacccaaga cctcatagcg ggtgcttctt ataaacccgg ggcctcccgg gccatgtgtg
    12721 gccaccaggt tctcaatgaa tgcctttagc tcatcccgtt tccccggatg agcctgtaat
    12781 tcgatgattg cttttattgc catattgtta tgaggattga ctgcggactt atctatttca
    12841 gccaaggcgg ctgcaggatc tgttggcgaa ttgaccccgg taataaacaa ggccatggca
    12901 ccccataaga gccccttttg tttcagcagt gggattaatt gcattaaata attcatgatc
    12961 ggagatttaa tttccttatt cgtatggctt ttcggcatcg cacgacgatg atccaacagg
    13021 gaccaacatt ttatgtaaat ataatgcgga cttcaccatt ttatttttta atactcatcg
    13081 tcgctactca tttttgatct tgccaattta aaagatgctg gaaaccgttc gggggcgggt
    13141 tttgaaccaa ggttgttcaa acccactaaa gtcaatcatt gtttaacagt ttttattcgt
    13201 acgtcccgca tactccagca ctcctttgtt ataaagcatt ttttaccaag ttcttaaatg
    13261 ccaaaagaaa tttatccaag ttgtccttgg tatggctgtc cgtcaggcgc ccctgttcgt
    13321 ctattttccc ctttacgccc tgaatcaaca gcgtggtgtc catcgtaaaa ttagccatgg
    13381 ccgttttcat gatgagctgc aattcttcgt gtcctttttc accgcttgcc gacgcagtaa
    13441 tcagtcccgt gggtttgccg gaaaaaacgg ttgttgaaat gcaccattca atagcatttt
    13501 taagtccgct tggaatactg aaaacatatt ccggcgtgca aattaaaatt ccttccgccg
    13561 cttcaattcg gtttctaaaa tctaaaatta cctccggggt attgttaaca gaacggtcgg
    13621 ggtcaaaatg cggaagtgtc ttcaggtcgt tgaaaacggt cagcttaaaa aagtctttcg
    13681 tccgctcctc cacgatatta ataagttttt gatttgaaga gtgttcgctg gcactcccgt
    13741 taattacaaa aatatttttc atcggtcagt ctgctgcggt gcgtcgttta cgaatctcta
    13801 ttgcatggtc tgggttaaat cgcaagattt aattattcag cctttttcga tcttgccgcc
    13861 ttttgttttt tcttgttctt taagcgttct tcataaaatt taaaactctc ctgaatcaaa
    13921 ccctctaatt cccggtccat gtcttttccg ttttcgatga tgatatggtg agtccaatag
    13981 cccttcctgg gttgaacaga ttcttcaatc ttgtcgcttt tgattctctt tccggtcaga
    14041 atggtcaggg taatggaatt ttcccgcctt ttctttatcc aggtttgcgg aagccaaacc
    14101 caaaggtatc ggtaatcctc tccgaacgat atttgcgtat tgtttaccac aattttcggg
    14161 gaacggtgtc tttcaataag actgcgaagg ctattaaata atttcaaaga ggcatctctt
    14221 ccatgaaaaa atttttgaat ttccttttca gtctttaacg tcatcatggt tcagtgctcc
    14281 caggcattga attttccagc tttcgtgtgt tcgtgcagtt tctcattcaa gtcacccaat
    14341 gaatctctta ctaactctct gcacgcattt aatttaggta aaaagtttta cttcctgcag
    14401 aactggccac cggccgactg caattctcag ttttattcaa tcctcaaccc tcaaccctca
    14461 tgcctcatgc ctcaaacctt caccgtcttc cacttccctt tcctgaaaaa ataataacag
    14521 gccagggcaa tagccgtttc ggccgcgggt atggctataa agacgccatc cgtacccaaa
    14581 tggaaaaccc cggaaaggac ataagccagg gggatctgga agcaccagaa accaacaaaa
    14641 ttgatccagg taggcgtgcg ggtatccccg gccccgttga aggcctgcat catcaccatg
    14701 cctataccgt aaaaaatata ccccgtgccg atgatgcgca aggcgtcaat gcccgtcttc
    14761 accacttcgg gctcctggct aaatatccgg atgatgggac ccggaatggc agtaaacagg
    14821 agggtgacca gggccataaa atagatattg taacgggcgg tcagtaggac tgatttttcc
    14881 gcccggtcgg gctggccggc gcccaggttc tggccgacga gggtggccgc ggcattgctc
    14941 agcccccagg cgggcaggat aaaaaagacc acgttgcgga tggccacctg gtagcccgcc
    15001 gaagcttccg taccgccggt ctcggccacc aggcgagtga gcacgatcca actgccggaa
    15061 gcgatgataa actggaaagt ggccggccag gcgatgtcca gcaaaccccg gatgaccttc
    15121 cagtcggctt taaactggct gagccggatc cggatctccc ccttgccatt gaacaaatgg
    15181 taacactggt acaatacgcc cgcacccctg cccagcgtag tggctatggc cgcccctttg
    15241 agtccatatc cttcccaggt ccctaaacca tagatcagga ggggacagag gacgatgttg
    15301 agcccgctgg ctatccacag gctgcgcatg gccatggccg cgtcacccgc cccccggaaa
    15361 atcccattga tcaggaaaag gaggatgatg accacacttc cgcccagcat aatgcgggta
    15421 aagggcgtcc cgtcgcgaac cacctccgga gtggcgccca taaactgcaa tatatgggga
    15481 gcatacacga tgcccaggat gctgatgacc agggtggcga ccagggaaat gatcagggac
    15541 tgcaccgcgg catggttggc cgcctccgga tttttttcgc cgatgcgcct ggcgatcatg
    15601 gccgtcgcgg ccatgctgag gcctatgccc gcggaataca ccagggtgac caccgcttcg
    15661 gtgagtccca ccgtggcaat ggcccccggc ccgagcttgc ctacaaaaaa catatcgacc
    15721 accgcaaaaa cgctttcgag gctcagttcc aggatcatgg ggatggccag caggacgatg
    15781 gcccgcttca tgctgccggc cgtataatcg gtatgttctc cttttaaagc ctggacaacg
    15841 agtttatagg tttccttaag ggaaggcatg gggtggtgca ggggcgctac aactggagca
    15901 tcagggacag ttgcagggtc cggttggtgt gtttttcggg gatgaggata aattctttgg
    15961 aaggattgag gcttttgtaa ctcacctcaa aatcgtcgtt gctcacatca gccatgccaa
    16021 cctgatagcg gatgccggca tacagacccc ggctcaggcg gtagtggacc gatcccgtaa
    16081 gcccgtaatc caggctgttg tatcggttgc catcgggttt ggtccggtat tcgtaataac
    16141 ctccgatccg ttccggcagc acgatctggt ccccttccag ccggatggtc tgggtgaccg
    16201 aagaggctac cgaaccggcc ttgtctttaa aataattctg gtccaggagg aaacgaatgg
    16261 gcccgacctg agcattgctg ttgtcccgct tcccggtata ttccaggtcg ccccggccgg
    16321 tcgagccgat gagggccgag gcataagccc cgacggccac atccagcctt tcaaaaaaca
    16381 gggtgtaaaa catgaggggc aggtccaggt aattgttgtt gatcacggaa ttcatcaccc
    16441 ggaccccgga ggtggggatg tattcctggt tttttttgat ggtgaagtag cttggcccgt
    16501 tgtaattgaa tttgctgcct ttctgggaaa aaatcaattc cccccggagt cccatgatgt
    16561 ccgaaaaacc gtatgaaaag accggcccaa catggaagcc gggagaagtc ccaaatcccg
    16621 cattttcttc cagagtaccg ttcatggtgg catagttgag gccgatccga agccccaggt
    16681 gagtttcctg gccgaaggcg gtcaggccgc ccaggacaca ccataaaagg atgacaataa
    16741 tttttttcat aaacttgagt aaggcgcaaa gatagggctt atgaattagc cgcgggattc
    16801 gctaagggat tgccactttc tgcccgcacg gcgggcgcaa agtgacaacc actcgcggca
    16861 cgcttgcaac ttactacgcg cacggcggcg caaaggtaaa gggaaagaaa gatggattgc
    16921 acactggcag cgctaagtga ttgc
//
LOCUS       DAOQXT010000104        16640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_3885_length_16640_cov_6.343805, whole genome shotgun sequence.
ACCESSION   DAOQXT010000104 DAOQXT010000000
VERSION     DAOQXT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16640
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_16640_cov_6.343805"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..551)
                     /locus_tag="PKM27_15480"
     CDS             complement(63..551)
                     /locus_tag="PKM27_15480"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNR08720.1"
                     /translation="MKWTALRAILKHLRDILLLPLPVTCLIPLAVYRYTPEYIPDFLP
                     VKIMGLVLFSLGVMLFCWSLFLFEKKGRGTLAPWSPKEKLITTGPYRFCRNPMTGGIL
                     LILVAEGLFFRSAPILIWAALFFILNFLFCKFREEPYLEKKFGATYQHYKSKVPGWWP
                     KI"
     gene            complement(565..732)
                     /locus_tag="PKM27_15485"
     CDS             complement(565..732)
                     /locus_tag="PKM27_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HNR08721.1"
                     /translation="MEKKKFVLRIDEQTMKALEKWAADEFRSLNGQLEYILTQALKEA
                     GRYPKYKEEKK"
     gene            complement(764..1624)
                     /locus_tag="PKM27_15490"
     CDS             complement(764..1624)
                     /locus_tag="PKM27_15490"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNR08722.1"
                     /translation="MKTEHVTQSFNGYLFLTISLLIFFSGLGIILFTEIRWLGVVLVV
                     LDSLFLFPGFFLVNPNESKVLLLFGEYKGTVKDNGFFWANPFYHKKRISLRAQNFDSE
                     RIKVNDKKGNPIMISGIVVWRVKDTYKAAFDVTDYHSFVKVQSEAALRELAGSYAYDN
                     MDDEGHEMTLRSGHDEVNHALETQLRARLAIAGIEVIEARIGYLAYAPEIASAMLRRQ
                     QAEAVVAARMKIVEGAVGMVEQALLHLSKKAIIELDEEKKAAMVSNLMVVLCSDKDAT
                     PIVNAGTLHQ"
     gene            complement(1776..3269)
                     /gene="glpK"
                     /locus_tag="PKM27_15495"
     CDS             complement(1776..3269)
                     /gene="glpK"
                     /locus_tag="PKM27_15495"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNR08723.1"
                     /translation="METYILALDQGTTSSRAILFDREGNIAGLEQKEFTQIFPQPGWV
                     EHDALEIWETQLWVAQNLLKKKGVSPGQVAAIGITNQRETTVIWDRITGQPIYNALVW
                     QDRRTAPICEKLKQEGLAEYITEYTGLVIDAYFSGTKIKWLLDQVPGAREKAEAGQLL
                     FGTIDTWLLWKLTGGKVHATDYSNASRTMLFNIRSLDWDPTLLKALHVPASLLPSVMP
                     SSALFGETEAVLFGEPVRISGMAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGTI
                     PYRSTKGLLTTIAWGLDGKVFYALEGSVFNAGSAIQWLRDGLGLIGNSSETEALATSV
                     PDAGGVYMVPAFTGMGAPYWDMYARGTIQGLTRGATRAHLVRATLESLAFQTKDVLDI
                     MAEESRIALDTLRVDGGASANNFLMQFQADILGTPVERPAVLETTALGAAYLAGLATG
                     YWTKGEIASRWQCDALFEPKLDMEKTGMLYRGWKKAVERSLGWIEGN"
     gene            3440..5146
                     /locus_tag="PKM27_15500"
     CDS             3440..5146
                     /locus_tag="PKM27_15500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNR08724.1"
                     /translation="MIPFSAKDRTGYFQKLADKKVHILVIGGGITGAGILLDAVNRGF
                     EAALIEKLDFGSGTSSRSTKLIHGGLRYLKQRQFALVRETGKERAIVFNNARHLVEKK
                     KMLLPIYKGGTLGTWTTSIALWIYDYLARVGRNEHKLMLKKETTRRTEPLLKQEGLKG
                     SGLYYEYHVDDTRLTLEVIKKAVENGGTALNYAEAMGFIMDQGRIAGVEVVDKLTGTP
                     FTIRADYVVNATGPWSDQVAGLDQQSGNIKRVVLSKGVHIVVPYYKLPVKQAVYFENR
                     KDRRMIFAIPWWGHTYVGTTDTAYDGDPDHIHATAEDVEYLVDAANSVFTRSPIRTED
                     ISSTWAGVRPLVYEKGKSLGELSRKDEVFKSPGGLITIAGGKLTGYRKMAERVIQLII
                     DDLKDHPDKPRPVLHEEALTDLSNISGSEFRSDEEFRKLKLYCLSLRHHLEVDEDTME
                     KLFHRYGTNTEKVVALAETFTSEVSDPYLRIELGSLLYGIRQEMVCSLSDHLVRRTSQ
                     LYFGRNQILKYLEPLSALLASELELSPSQLLSDKNSFLKEYEAVMSWQKEKNPVTDTF
                     IH"
     gene            5317..6333
                     /locus_tag="PKM27_15505"
     CDS             5317..6333
                     /locus_tag="PKM27_15505"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNR08725.1"
                     /translation="MNRRAFTKSLLGSGMAYGIGAQSNMKKPTYLKSGDQVGLICPAG
                     PISDERLVRSINNLLNLGLKPIPGNYILNQNGYLSGTRTERLADLHQMYRDEQIRAVW
                     CIRGGFGCTHLLPHLDYDLVQRSNKLLLGYSDVTALHCGLYTRLKISNLHSPVASSEF
                     TPYTLEQLQKIIFGMPGQVIRIGASPENDQLFNEGNTVYERYVIREGIGRGVLWGGNL
                     SLLSALCGTPYLKINRDFLLFVEDIDEAPYRIDRMLTQLMQVLPLHRLKGVVLGVFEG
                     CEKKANTPSQSLKEVMLDRFSDFDVPVMYGFPFGHIKNQCTLPFGAEAVMDTGSFQLT
                     VKLP"
     gene            6335..7363
                     /locus_tag="PKM27_15510"
     CDS             6335..7363
                     /locus_tag="PKM27_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNR08726.1"
                     /translation="MKLTIHTYILKTRKVFTTSHGSADHRRCLIVELEHEGCKGYGEA
                     TEILYYGKNLEAMVRLVQGNKEWIESLPMADPLDRYLEFFSRFSEDRFVLCALDVAYH
                     DLYGKLKGQSLTRMWGLDRSHNPLSSLTVAIDETEKMLEHIDQNPWPVYKVKLGTPQD
                     MEIMQAIRRHTDHPLRVDVNEGWSAAQTIAYSSTLRDLEVEFIEQPIPAREHDSMAEV
                     FRKSALPLFADESCHIPEDVPRCLQHFHGINIKLMKCGGLSPALEMIRLARTLGLKIM
                     IGCMTETSVGISALGQILPLVDYADMDGALLISNDPAAGVHLEDGRAVYPDEGGTGAR
                     LITENPLA"
     gene            7364..8410
                     /locus_tag="PKM27_15515"
     CDS             7364..8410
                     /locus_tag="PKM27_15515"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR08727.1"
                     /translation="MYYASHIPSRTTHVGGEEYLFFSGTAYLGMHARPEFGDLVYEGI
                     RNWGTNYGASRLGNVSIPVFDQAESKLAAWLGTSSALVVSSGTLAGRLVLEVLSEDYE
                     THYSPIAHVAINPSYKKHEPHRFDAWIEKTLQNIRVSDKEKHLITFNSIDSLTGIQPT
                     LDWVDAIPGNKEVLLIADDSHGIGVMGTEGRGNFPLLLRKHPNALLIASLGKAMGLPA
                     GLIAGSGEIVQRAKAHPLFGGSSPMVPAYAHAFLHSDELYAKARRQLFENMKRFEEGA
                     RDSGLITGSRNFPVYCTHSHELAPFLEKRKIRISHFAYPSPADNLYTRVVVNALHTQE
                     DMERLIEGIKGFRG"
     gene            8524..10869
                     /locus_tag="PKM27_15520"
     CDS             8524..10869
                     /locus_tag="PKM27_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNR08728.1"
                     /translation="MNMHGIKPFFCFILILLSSCKKEPGHTGSLFEKRAVDLHPSPLP
                     LSPEESIKKIQLPPGYSVELVAAEPLIRDPVGMAWDGNGNLYVVEMSTFMMDARATDQ
                     YKPYNRIVRLVDTDWDGRMDRSTIFIDSLVLPRVVLPVRDELIVGVTNVQHLYAYIDT
                     NDDGRADQKRLVFKNDAIDSRNMEHQNGGLIWNLDNWIYPSRDNLRFKYKEGRLIADT
                     LIDNMIGQWGMTSDNYGRLFYSEAGPGLPVVQFNQMPAYGSLNFSDQYPADFAVPWPI
                     MSTVDAQGGPQVLRPSDSTLNQFTSGCGQSIFRGDRMPEDMQGDYFIAEPVARVIKRG
                     KVIDRDGKRMVENVYQGQDWLASADFNFRPVNTYTGPDGCFYIVDMYRGIIQEGEFAE
                     EDSWLNRKIKALGLEKNKGYGRIYRVVHRDYRRDAEKPRLLEKSAMDLLPYLGHPNGW
                     WRDQAQQLLVLKQDKSIYEPLLQMVRNAPGALIRDKALARIHALWTLDGLGLAHTGIL
                     SEALKDEHAQVRKAAVWIGEAWIRQNDTAFINRLGLLKDDPSPDVRIQLYLSLRTSKN
                     KKAESIVRDILRENPDNEMIRYSHSVFEESYARAEAEKARISRLSPAEKQLIALGATR
                     YKQLCSTCHGMDGKGISIGGAPRIAPPLAGSPRVRGDKIMLIQILLYGLKGPVDNISY
                     PGSMLPYKQQDDEWIASVLSYIRNSADLGNASSIVTREEVEDIRATAAIEDGHIPDLR
                     MLEIYKLGRGEAQNWSKGKPGSGGQRWGGHFRSMKDSTGRN"
     gene            10904..11728
                     /locus_tag="PKM27_15525"
     CDS             10904..11728
                     /locus_tag="PKM27_15525"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR08729.1"
                     /translation="MNWLKRLTLLGLIPASLWPVLGQESYINGIGMEFIQVPAGEMIV
                     GRFQPTVSRMKAWGSDQPLNEEVYGQAVRMARLEDRDGFRVKIQAPYYIGKYEVTQDQ
                     WQKVMGGNPSFFNKSRVGDESGRHPAEGISWKAAQRFIHKLNKLEQGKARYRLPTEFE
                     WEYAARAGKKDDISWREILESAVIASPSTAEAGSKKPNSWGIYDMLGNVWEWVEDIYN
                     EKIFADPVPPKKGREHVLKGASFFGDVKNATYMTHAGGPGSGYDIGFRLVMEKHTN"
     assembly_gap    11757..11856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11857..12553
                     /locus_tag="PKM27_15530"
     CDS             <11857..12553
                     /locus_tag="PKM27_15530"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR08730.1"
                     /translation="KVKVKVKVLSFLLVTAMASCLKAQLPAGFKSIFNGKNLKGWHIS
                     RTNHQGTTPDVQVRNGVLELRQSPFGQGGVLLTNKQYSDFELYLEVNIDSFSNGGLFL
                     RSNEGGAAYQVELALPGGTGDLLGERIPVSNPAKASGRENVWKPNDWNSFRIRMQGTV
                     PKICLWINDRLIYEVEQTRNDFIAGATRGMVGLQCHWTALYSPAAESELMPLDAWRPG
                     AVHRFRNIGIRKR"
     gene            12679..12873
                     /locus_tag="PKM27_15535"
     CDS             12679..12873
                     /locus_tag="PKM27_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08731.1"
                     /translation="MKWPILIYLTWSVLIAACKKDGIGQYDLPDKVLFTHLPTELDQM
                     LEFGAIGQIQVIPKVQGGFL"
     gene            12916..13779
                     /locus_tag="PKM27_15540"
     CDS             12916..13779
                     /locus_tag="PKM27_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08732.1"
                     /translation="MSEGVIYNIRKEERTVIEQDAPADIRGKTFEDYALDIALTGRSR
                     MYYGHVSKLSNGVLEAAGAIQAGRGVENKVSIPVSAGQVLGYIGIQPGFDIGMWDLDR
                     EGFFSNPDRYTREYRASVSYTDYLPGALREQIWNINPRTVEPRGGKITYDVEGTLAGN
                     WFIPGTKSLTEWSKQLVIARHEKYADRVTIADASPLLDGDGAKNVGRASYIWWIKGNI
                     PLPETVTPASGKIKYAVADWWKFTHQEGNPDGTLLIRMENPRQIRYQYFAGKMPDELT
                     EFSGEARTYVR"
     gene            13882..14964
                     /locus_tag="PKM27_15545"
     CDS             13882..14964
                     /locus_tag="PKM27_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR08733.1"
                     /translation="MNNQTKPIRVLVAGAGNMGGSHATAYHNMEGFEICGLVSTGKSK
                     ELLNQKLGGGYALFSDYSKALELTRPDAVSINTYPDTHESYALQAFDQGCHVFMEKPI
                     ADTVAGAERVAEAARQAGKKLVIGYILRHHPSWIKFIEIAQQMGKPLVMRMNLNQQSH
                     GYMWTVHRNLMKSLSPIVDCGVHYIDVMCQMVRSKPIQVGAIGARLTEAIPAGNYNYG
                     QLQIRFEDGSVGWYEAGWGPMVSETAFFVKDVFGPKGAVSIVARDAGGSGKSSSIDAH
                     TKTESLRIHHAELNEQDEFVRPDEWLDMTDEPGHQELCNREQAYFLKAIREDLDLTHH
                     VQDAVDSLRIAFACDESVRTGQVVKL"
     gene            complement(15050..16018)
                     /locus_tag="PKM27_15550"
     CDS             complement(15050..16018)
                     /locus_tag="PKM27_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08734.1"
                     /translation="MKNFLYLNACLFLLISCRDSSPSGQAAGSEKGYFIAYNVYIPDT
                     TKDDWEVMLMNMDGSGKRNLTRNDDVAWTYYAFQDKLYFISDRDTCYRCFFLYETDPG
                     GQSIRKVSDLRLEDSWMSSRHHGEEMVVTGRLGPTTRFQLFIVNTKSGQYSQITSDTA
                     ARYGDPCFSPDGSQIVYAYKQNKRDRNSHEELFIMNSDGSGSRQLTHYPEDNVSAKEP
                     GYKAGAPRWHPTEHFISYISMQDGRHQICAVNPEGSKQWKITTGPKGEGWHDWSPDGK
                     WLTYDSSDPNGRQYHILLMNWQTKEVKQLTDTTYRSQLAPVFVNSQ"
     gene            complement(16131..>16640)
                     /locus_tag="PKM27_15555"
     CDS             complement(16131..>16640)
                     /locus_tag="PKM27_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR08735.1"
                     /translation="GPLGEYTDAVVDGEHSFRKDILRVREALLPLPGAWEKFALAEKL
                     LLAQYGPDLRLNPCVDYAVEQILLHPAQTTIANLSVKIGYSHKHLIRLFRDHTGLSPK
                     EFLKIIRFQKAISRIDAPAAGGWTAIAAASGYYDQAHFIHEFKNYSGFTPAQFVEQQR
                     AFTNYVALE"
BASE COUNT         4111 a         4115 c         4499 g         3815 t
ORIGIN      
        1 acttttcagt gactcatttt tatattgcca tcaaccgtta gcaagttagc aagttggtaa
       61 gttcagatct tgggccacca gcccggcact tttgatttat agtgctggta tgttgccccg
      121 aattttttct cgagataagg ctcttctctg aatttgcaga acagaaagtt gaggataaaa
      181 aacagggcag cccaaattaa aataggagcc gatctgaaaa acaggccctc ggcaaccagg
      241 atcaggagga ttcccccggt catgggattc cggcaaaacc ggtaagggcc ggtggtgatc
      301 agtttttcct tggggctcca gggagcaagg gtgcccctgc cctttttttc aaaaaggaaa
      361 agggaccagc aaaacagcat gactccaagc gagaataaca ccagacccat aattttgacg
      421 ggaaggaaat ccgggatata ctccggcgtg taccggtaaa ccgccagggg gatgaggcag
      481 gtcaccggca gggggagcag gaggatatcc cggaggtgct ttaggatggc ccggagtgca
      541 gtccatttca taaggcgatc gcgttcattt tttttcttct ttgtacttcg gatagcggcc
      601 ggcctctttc agggcctggg tgaggatgta ttcaagttgg ccgttgaggc tccggaattc
      661 atcggcagcc catttttcca gcgccttcat ggtctgctcg tcgatgcgga ggacaaattt
      721 tttcttttcc atgatctggt ttaaaaccgt tcacccgacg gcatcactgg tgcagggtcc
      781 cggcattgac gatgggggtg gcatctttgt cggaacagag caccaccatc aggttgctca
      841 ccatggccgc tttcttttct tcatccagtt caatgatcgc cttcttggaa agatggagca
      901 aggcctgctc gaccatgccg acggcgcctt ccacgatctt catgcgggct gcaaccacgg
      961 cctcggcctg ctgccggcgc aacatcgcac tggcaatctc cggggcatag gccaggtagc
     1021 cgatcctggc ttcgatcact tcgatcccgg cgatcgccag gcgcgcccgc aattgagtct
     1081 ccagggcgtg gttgacctca tcatgcccgg agcgcagcgt catctcatgc ccctcatcat
     1141 ccatattgtc ataggcatag gatcccgcca gttcacgcag ggcggcctcc gactgcactt
     1201 ttacaaaact gtggtaatcc gtcacatcaa atgcggcttt gtaggtatcc ttcacccgcc
     1261 agaccacgat cccgctgatc atgatcggat tgcctttttt atcattgacc ttgatccgct
     1321 cgctgtcgaa attttgcgcc ctgagggata ttcttttttt gtggtaaaag ggattggccc
     1381 agaaaaagcc attgtccttg accgtgcctt tatactcccc gaaaagtaaa aggaccttgg
     1441 attcattggg gttgaccagg aaaaaccccg gaaagagaaa aaggctgtcc agcacaacca
     1501 gcaccacccc taaccaccgg atttcggtaa acaaaataat ccccagtccg gaaaagaaga
     1561 tcaggaggct gatggtgagg aaaagataac cattaaatga ttgggtaaca tgttcggttt
     1621 tcattttgat attaatttga tatcaaaagt atagcatttt atcttttttg tcaagggggg
     1681 agaaaaaaaa tcttcagcag cagctttttt aaggtaatag aatgaccagg cccgaagcct
     1741 ccacatcaat ccttaattct taatttctta attccttaat tcccttcaat ccaccccaaa
     1801 ctcctctcca ccgccttctt ccagccccgg tagagcatcc cggttttctc catatccagt
     1861 tttggttcga aaagggcatc gcattgccag cgactggcta tttcaccctt ggtccagtaa
     1921 ccggtggcca ggccagcgag gtaagcagct cccagggcgg tggtttcaag gacagccggc
     1981 ctttccaccg gggtgcccag gatatcggcc tggaattgca tgaggaaatt attggctgaa
     2041 gcgccgccgt caacccggag ggtgtccagt gcgatgcggg attcttccgc catgatgtcg
     2101 aggacatctt tggtttgaaa agccagggat tccagggtag cccggaccag gtgggcccgg
     2161 gtggccccgc gggtaaggcc ctggatggtc ccgcgggcat acatatccca gtaaggagcc
     2221 cccatgcccg tgaaagccgg aaccatataa accccaccag catccggaac cgaagtcgcc
     2281 agggcttccg tttccgatga gtttccgatc aggcccaggc catctctcag ccattgaatg
     2341 gcggagcccg cattaaaaac gctgccctcc agcgcataaa atactttgcc atcaaggccc
     2401 caggcaatgg tggtgagcag gcctttggtc gaccggtaag ggatcgtgcc cgtattcatc
     2461 agcataaagc agccggttcc ataggtgttt ttggccatgc cgggttcaaa acaagcctgg
     2521 ccaaacaggg ccgcctgttg gtcgccggcc atgccgctga tccggacggg ttcaccaaac
     2581 agcaccgcct ccgtttcccc gaaaagagcg ctcgagggca tcaccgaagg aagcaggctg
     2641 gccggtacat ggagggcttt gagcagggtg gggtcccagt ccagacttcg gatattgaaa
     2701 agcatggtgc gggaagcatt gctgtaatcc gtggcatgca ccttgccgcc agtcagtttc
     2761 cacaaaagcc aggtatcgat ggttccaaac aaaagctggc cggcttctgc tttctcccgg
     2821 gcgccgggca cctggtccag cagccatttt atttttgtcc ctgaaaaata ggcatcgatg
     2881 actaaaccgg tatattcagt gatgtattcg gccaatcctt cctgcttgag cttttcacaa
     2941 atgggggcgg tccgccggtc ctgccagacc agggcgttgt aaatgggttg tccggtgatc
     3001 ctgtcccaga tgacggtggt ttcccgctga ttggtaatgc caatagccgc aacctgaccg
     3061 ggacttaccc cttttttctt caataggttt tgggcgaccc aaagctgcgt ttcccatatt
     3121 tcaagggcat cgtgctccac ccagccgggc tggggaaaga tctgggtgaa ttctttttgt
     3181 tccaatccgg cgatattgcc ctcccgatcg aaaaggattg cccgcgaact ggtagtgccc
     3241 tggtccaggg cgaggatata ggtttccatg gccatggaag taaaaatagg aattaaggat
     3301 tattcgttca tgaattaaac gccagctaac ctcaaccctc aatcctcaat cctcaatcct
     3361 caatcctcaa tcctcttacc tctaacctca tatattatac ttttatttaa tacaattacc
     3421 taaatttgtc tcgcagatca tgattccatt tagtgcaaag gaccgtaccg gatattttca
     3481 aaaactggct gataaaaaag tccatatact ggttatcggg ggtgggatca ccggcgccgg
     3541 catcctgctg gatgcagtca accggggctt tgaagcagcc ctgatcgaga agcttgattt
     3601 tggaagcggg acttccagcc gttccaccaa actgattcat gggggactgc gttacctgaa
     3661 acaaaggcag tttgcactgg tcagggaaac cggcaaggaa agagccatcg tatttaacaa
     3721 cgccaggcat ctcgtggaaa agaaaaaaat gctcctcccg atctacaagg ggggcaccct
     3781 gggcacctgg accacctcca ttgctctatg gatctatgac tacctcgcca gggtgggccg
     3841 gaatgagcat aaactcatgt tgaaaaaaga aacgacccgc aggacggagc ccttgctcaa
     3901 gcaggaagga ctgaaaggtt ccggccttta ttatgaatac catgtggacg atacccgcct
     3961 gaccctggaa gttattaaaa aagcggttga aaacggaggt actgccctga attatgcgga
     4021 agccatggga tttattatgg accaggggcg aatcgcgggc gtagaagtcg tggataaatt
     4081 gacaggcacc ccttttacca taagggcgga ttacgtagtc aatgccaccg gaccttggtc
     4141 ggaccaggtc gccgggcttg accaacaatc cggaaatatc aaaagggtag tcttgtccaa
     4201 aggggtgcat atcgtggtgc cttattataa attgccggtc aaacaggccg tatattttga
     4261 gaaccgcaaa gaccggcgca tgatcttcgc cataccctgg tgggggcata cctatgtcgg
     4321 tacgaccgat accgcttatg atggcgaccc ggaccatatc catgctacag cggaagatgt
     4381 tgaatatctc gtggatgccg ccaattcggt cttcacccgg tctccgatcc ggacggagga
     4441 catcagctcc acctgggcgg gagtcaggcc cctggtttat gaaaaaggca agtccctggg
     4501 ggaattgtcc cgcaaggacg aagtatttaa aagtcccggg ggactgatca ccattgccgg
     4561 agggaaactg accggctacc gtaaaatggc tgaaagggtg attcaactga tcatcgatga
     4621 cctcaaagac catccggaca aaccgaggcc ggtccttcat gaggaagccc ttaccgacct
     4681 gtccaatatc agcggttcgg aattcagatc ggatgaggaa ttcagaaaac tgaagttgta
     4741 ttgcctctcc ctgaggcatc acctcgaggt ggatgaggac accatggaaa aactgttcca
     4801 ccgttacgga accaacacgg agaaagtggt ggccctggcg gaaaccttca cttcggaagt
     4861 ctccgatcct tatctccgga ttgaactggg aagcctgctc tatggcatcc ggcaggaaat
     4921 ggtttgcagt ctttcggatc acctggtgcg cagaacgagc cagttgtatt tcggaaggaa
     4981 ccagatcctg aaatacctgg aacccctgtc ggccttgctg gcctccgaac tggaattaag
     5041 cccctcccaa ctcctcagcg ataaaaactc cttcctcaaa gaatatgaag ccgtgatgtc
     5101 ctggcaaaaa gaaaaaaacc ctgtaacgga tacttttatc cattaagggt tgcatatgaa
     5161 aagactcact cttaattctt gaaataaagg tatcggtcat tcaaaaagcc aaaccagaat
     5221 tatggattaa atgaaaggaa tcgtcgacaa agattttcaa tacccgcaat gaagggcaaa
     5281 acaaccggac acggaaatga agaaatcagg aagaaaatga accgtagagc atttaccaag
     5341 tccctcctgg gttccggaat ggcatatggt attggggcac aaagcaatat gaaaaaaccg
     5401 acttatctga agtccgggga ccaggtcgga ctgatctgtc ctgcggggcc catcagcgat
     5461 gaaaggctgg tccggagcat caacaatctt ttgaatctcg ggctgaaacc catccccgga
     5521 aattatatcc tgaatcaaaa cggttacctg tcaggcaccc ggacggagcg ccttgccgac
     5581 ttgcatcaga tgtatcgcga tgagcagatc cgggcggtct ggtgcatacg cggcggtttt
     5641 ggctgcaccc acctgttgcc ccacctcgac tatgacctgg ttcaaaggtc caacaaactg
     5701 ctgctgggat acagcgatgt caccgcactg cactgcggcc tgtacacccg ccttaagata
     5761 tcaaacctgc atagcccggt ggcctcctcc gaatttacgc cctataccct ggaacaactg
     5821 cagaaaatca tattcggcat gccgggccag gtcatccgca ttggcgccag cccggaaaat
     5881 gaccagctgt tcaacgaagg caataccgtt tacgaaagat atgtgattcg cgaaggcatt
     5941 ggcagggggg tgctttgggg gggcaatctt tcccttctct ccgcattgtg cggtaccccc
     6001 tatctgaaaa tcaatcgtga ttttcttctg tttgtggaag acatcgatga ggccccttac
     6061 cggatcgacc gtatgctgac ccagctgatg caggtattgc cgctgcacag gctgaagggg
     6121 gtggtcctgg gggtgtttga aggttgtgaa aagaaagcca ataccccttc ccagtcgctg
     6181 aaggaagtca tgctggaccg gttttcagat tttgatgtgc cggttatgta tggtttccct
     6241 tttggccata tcaaaaacca atgcaccctg ccttttgggg ccgaagccgt gatggatacc
     6301 ggttcatttc aattgaccgt aaaactgccc tgagttgaaa ctgaccatcc acacctatat
     6361 cctgaaaacg cgcaaggtgt tcaccacttc gcatggcagt gcggatcatc gcaggtgcct
     6421 gatcgtcgaa ctggagcatg agggatgcaa gggatacggg gaagccaccg agatcctgta
     6481 ttatgggaaa aacctggaag ccatggtccg cctggtgcaa gggaacaagg aatggatcga
     6541 atcccttccg atggcagatc ccctggaccg ctacctggag tttttcagcc ggttttcaga
     6601 agatcgattt gtgctttgcg ccctggacgt agcctatcac gatctctatg gaaaactgaa
     6661 aggacagtcg ctgacccgca tgtggggcct ggaccgaagt cataatcctc tttccagcct
     6721 tacggtggcg attgacgaga cggaaaaaat gcttgaacac attgatcaga atccctggcc
     6781 ggtttataag gtcaaactgg gcaccccgca ggatatggaa atcatgcagg ccatccggag
     6841 gcatacggat caccccctga gagtggatgt caacgaaggt tggtcggctg cgcaaaccat
     6901 tgcttattct tccaccctcc gggatcttga agtggaattc atcgaacaac ccatccctgc
     6961 cagggagcat gactccatgg cggaagtgtt ccggaaaagc gcccttccat tgtttgccga
     7021 tgaatcctgc catatccctg aggatgtgcc ccgttgtctt caacatttcc atggcatcaa
     7081 tatcaagctg atgaaatgcg ggggactcag ccccgccctg gagatgatcc gcctggcccg
     7141 gacgcttgga ctcaaaatca tgatcggttg tatgacggaa acttcagtag gcatttccgc
     7201 cctgggtcag atcctgcccc tggtggatta tgcggatatg gacggggcct tgctgatctc
     7261 caacgatcct gctgccggcg tgcacctcga ggatggccgg gcggtttatc ctgacgaagg
     7321 gggaaccggt gcgcggctga tcacagaaaa tccccttgcc tagatgtatt atgcttccca
     7381 tatcccttca aggaccaccc atgtgggcgg tgaggaatat ctttttttca gcgggaccgc
     7441 ctacctgggc atgcatgccc gccccgaatt tggggacctg gtttatgaag gcatcagaaa
     7501 ttggggtacc aattacggag caagcaggct gggcaatgta tccattcccg tttttgacca
     7561 ggccgaatcg aagctggctg cgtggctcgg gacttcatcg gccctggtgg tctcttcagg
     7621 aactttggcc ggcaggttgg ttcttgaagt cctgagtgaa gattacgaaa cccattattc
     7681 gcccatcgcc catgtcgcca tcaatccctc gtataaaaaa cacgagcccc atcgcttcga
     7741 cgcctggata gaaaaaaccc tgcaaaacat ccgggtcagc gacaaagaaa aacacctgat
     7801 cactttcaat tccatcgatt cgcttacggg gatccaacct accctggact gggtggacgc
     7861 catacccgga aacaaagaag tattgctgat tgcggacgat tcacacggca ttggcgtaat
     7921 gggtacggaa ggccggggaa attttccact cctgttgagg aagcatccga atgccctcct
     7981 gatcgcctcc ctgggcaagg ctatgggcct gccggcgggg ctgatcgcgg gatccgggga
     8041 gatcgtgcaa agagccaaag cgcatccctt gtttggagga agttcaccta tggtgccggc
     8101 ctatgcccat gcctttctgc acagcgacga attatatgcc aaagcacgcc ggcaactttt
     8161 tgaaaatatg aaacggtttg aggaaggggc ccgggacagc ggcctgatta ccggcagccg
     8221 gaacttcccg gtatattgta ctcactccca tgagctggcc cccttcctgg agaagcgaaa
     8281 gatccggata tcccattttg cctatcccag tcctgcggat aatttataca cccgggtggt
     8341 ggtgaatgca ttgcatacgc aggaggatat ggagaggttg attgagggga ttaaggggtt
     8401 tagaggttga ggttgaggtt aaggttgagg ttaaggttga ggttaaggtt gaggttaagg
     8461 ttgaggttga ggggcctgaa tttcaaatgg tatccaggtt tatccttgta aatttagctt
     8521 ttcatgaata tgcacgggat caaaccattt ttctgcttta tccttatcct cctgtcatcc
     8581 tgcaagaagg aacccgggca taccggctcc ctttttgaaa agcgggccgt ggacctccac
     8641 ccttcgccct tgcctctaag cccggaagag agtataaaaa aaatacaact gccaccgggt
     8701 tattccgtgg aactggttgc cgcggaaccc ctgatccgtg atccggtggg catggcctgg
     8761 gatgggaatg gaaatctcta tgtcgtcgag atgagcacgt tcatgatgga tgccagggct
     8821 acggaccagt acaaacctta caaccggatt gtcagattgg tcgataccga ctgggacggc
     8881 cgcatggacc ggtcgaccat ttttattgac agcctggtcc tgccgcgtgt agtgttgccg
     8941 gtgagggatg aactgatcgt cggggtcacc aacgtccagc atctctacgc ttatatagac
     9001 accaatgacg atggcagggc ggatcagaaa cgcctggttt ttaaaaatga cgccattgat
     9061 tcccgcaata tggaacatca aaacggcggc ctgatctgga acctcgacaa ttggatttat
     9121 ccgagcaggg acaacctgcg ttttaagtat aaagaaggcc ggctgatcgc cgataccctg
     9181 atcgataata tgatcgggca gtggggcatg acttcggaca attatgggcg cttgttttat
     9241 tccgaagccg ggcccggcct ccccgtggtc caatttaacc agatgccggc ctacggctct
     9301 ctcaatttca gcgaccagta tcccgctgat tttgccgttc cctggcccat catgagcacg
     9361 gtggacgccc agggcggtcc ccaggtgttg cggccttccg acagtacact caatcaattt
     9421 acttcgggat gcggtcaatc catcttcagg ggagaccgga tgccggaaga catgcagggg
     9481 gattatttta ttgccgagcc ggtggccaga gtcatcaaaa gggggaaggt cattgaccgg
     9541 gatggaaaaa ggatggtgga aaatgtttac caggggcagg actggctggc ttctgcggat
     9601 ttcaatttca ggccagtcaa tacctatacc ggcccggatg gttgttttta tatcgtggac
     9661 atgtaccgcg gaatcatcca ggaaggggaa ttcgcggaag aagattcctg gctcaaccgg
     9721 aaaataaaag ccctcggcct ggagaaaaac aaaggatacg gcaggatata ccgggtggtc
     9781 caccgggatt accggcggga tgccgaaaag ccgcgcttgc tggagaagtc ggctatggat
     9841 ttattgccct atctcgggca tccgaatggc tggtggaggg accaggctca gcaactgctg
     9901 gtcctcaaac aggataagtc gatttatgaa ccgctcctgc aaatggtaag aaatgccccg
     9961 ggagctttga ttcgcgacaa ggccctggcc cgcatccatg ccctctggac tctggatggt
    10021 ctcggcctgg cccatactgg catcctttca gaggccctga aggatgagca tgcccaggtg
    10081 cgaaaagccg cggtctggat cggggaagca tggatccggc aaaacgatac agcctttata
    10141 aaccgattgg gtcttttgaa ggacgaccct tcgcctgacg tccgcattca attgtatttg
    10201 tccctgcgca ccagcaaaaa caagaaggca gagtccatcg tgagagatat cctccgtgaa
    10261 aatccggaca atgaaatgat ccggtattcc cattcggtat ttgaggagtc ttatgccagg
    10321 gccgaggcgg aaaaggccag gatcagccgg ctcagcccgg cggaaaagca gttgattgcg
    10381 ctgggggcca ctcgctataa acaattatgt tccacctgtc atggcatgga cggtaaaggg
    10441 atatccatcg gaggcgctcc ccggatagct ccgcccctgg ccggctcacc ccgggtcagg
    10501 ggtgacaaga tcatgctgat ccagatttta ttgtacgggc tcaaagggcc ggtggacaat
    10561 atttcttatc cgggctccat gctcccctat aaacaacagg acgatgaatg gatcgcttcc
    10621 gtactgagtt atatccggaa cagtgccgat ctcggtaatg cctcctccat tgtgacccgg
    10681 gaggaagtcg aagacatccg cgctacggca gccattgaag atggccatat cccggacctg
    10741 aggatgcttg aaatatacaa actgggaagg ggagaggccc aaaactggtc aaagggtaaa
    10801 ccggggagcg ggggacaacg ctggggaggg catttcagat cgatgaaaga cagcactggt
    10861 aggaattaaa gaattaagaa ttaagaatta agaaataaga attatgaatt ggctgaaaag
    10921 gctgaccttg ctcggtttaa ttccagctag cctgtggcca gtattggggc aggaatctta
    10981 tatcaacggc ataggcatgg aatttattca ggtaccggcg ggggaaatga ttgttggccg
    11041 gtttcaaccc acggtgtcgc gaatgaaagc ctggggctca gaccagccgc tgaatgaaga
    11101 ggtgtatggg caggcggtgc ggatggccag gcttgaagac cgggatggat ttcgggtgaa
    11161 gatccaggct ccttattata taggaaaata tgaagtgacc caggaccaat ggcaaaaagt
    11221 gatgggcggg aatccgtcgt tttttaataa aagcagggtc ggggatgagt cgggcaggca
    11281 cccggcggaa ggcataagct ggaaggccgc ccaaaggttt atccataaac tcaataagtt
    11341 ggagcaaggt aaggcccggt accgcttacc cacggagttt gagtgggaat atgccgccag
    11401 ggccggtaaa aaggatgata tatcctggag ggaaatcctg gagtctgcgg tgattgccag
    11461 cccttcgacc gcggaggccg ggtccaaaaa gccgaattcc tggggtattt acgatatgct
    11521 gggtaatgta tgggaatggg tggaagacat ttacaacgaa aaaatattcg ccgatccggt
    11581 ccctccaaag aaaggcagag agcatgtcct caaaggggct tccttttttg gcgatgtaaa
    11641 aaatgcgact tatatgaccc atgcgggagg tccgggcagt ggttatgata taggtttcag
    11701 gttggtcatg gagaagcata caaattgaag gtgatggatt ttggaattga ggatgannnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaag gttaaggtta aggttaaggt
    11881 attgagcttt ttattggtga ctgcaatggc ctcttgcctt aaggcgcaat tgcctgccgg
    11941 attcaaatcc atcttcaatg gtaaaaacct gaaaggctgg catatcagcc ggaccaacca
    12001 ccagggaacg accccggatg tgcaggtgcg gaatggagtg cttgagttgc gacagtcgcc
    12061 ttttggccag ggcggggtat tactcaccaa taaacagtat tcggattttg aattatacct
    12121 ggaagtaaac atcgattctt tttccaacgg gggcctcttc ctgcggtcca atgagggcgg
    12181 ggccgcatac caggtggagc ttgccctgcc cgggggtacg ggagacttgt tgggggaaag
    12241 gattccggtg agcaatccgg ccaaggcaag cggaagggaa aacgtctgga aaccgaacga
    12301 ctggaattca ttccgcatcc gcatgcaggg gaccgtcccg aaaatctgtt tatggatcaa
    12361 cgaccgcctg atttacgaag ttgagcagac ccggaacgat tttatcgccg gggccacccg
    12421 gggtatggtc ggccttcaat gccactggac cgctttatat tctccggctg cggaatctga
    12481 actcatgccg ctggatgcct ggcggccggg agccgtgcac cggttccgga atatcgggat
    12541 ccgaaaacga taggtcttat tcagaaagga atataccctg aaggacgggg atgcagcata
    12601 agacaattat ttaccggatg aaatcgttaa tttagaggtg cacaccatga aataggacaa
    12661 tgcgccaaat aaagggtcat gaagtggcca atactaatat atctgacctg gtctgttctg
    12721 atcgctgcct gtaaaaagga tggaataggc caatatgatc ttcccgataa ggttctgttc
    12781 acccacttgc ctacggaact cgatcaaatg ctggaattcg gggccatcgg ccaaatccag
    12841 gtcatcccaa aagtgcaagg aggtttttta taaaggaaac cttatgcgcc aaatccgcta
    12901 atcccggtat atgccatgtc tgaaggggta atctacaata tccggaagga agaaaggaca
    12961 gtcatcgaac aggacgctcc cgcggatatc cggggaaaga cctttgagga ttatgccctg
    13021 gatattgccc tcaccggtcg atccaggatg tattatggcc atgtgtcaaa gctttcaaac
    13081 ggagtattgg aagcagcggg agcgattcag gcagggaggg gggtggaaaa caaggtatcc
    13141 attccggtca gtgccggaca agtccttgga tacatcggca tccaacccgg ttttgatatc
    13201 ggaatgtggg acctggatcg ggaaggtttt ttttcaaatc ctgatcggta caccagggaa
    13261 taccgggcct cggtgagtta taccgattac ctgcccggtg ccctcagaga acaaatctgg
    13321 aatatcaatc ccaggacggt ggaaccccgg ggtggaaaga ttacctatga tgtggaagga
    13381 acccttgccg gcaattggtt tattcccggg accaagtccc tgaccgaatg gagtaaacaa
    13441 ctggtgatcg cccgacatga aaaatatgcc gatcgcgtca ccatagccga cgccagccct
    13501 cttctcgatg gagatggagc aaagaatgtc ggccgggcct cctatatctg gtggatcaaa
    13561 ggcaatatcc ctttgccgga aaccgtgact cccgcctctg gaaaaataaa atatgccgtt
    13621 gccgattggt ggaaattcac gcaccaggaa gggaacccgg acggcaccct gctgatcagg
    13681 atggaaaatc cgcgacagat ccgttaccaa tattttgccg gcaaaatgcc ggatgagttg
    13741 actgaatttt caggtgaggc acgaacctat gtgcggtgat tgaataaaat attattgcaa
    13801 atctgccgcc ggtccgtctg gttagggcaa gtggaattaa ccggaatttt tacggagatt
    13861 tgcccttcaa caaaccattc aatgaataac caaaccaaac ccatccgcgt cctggtcgcc
    13921 ggcgccggca atatgggtgg atcacacgcc acggcctatc ataatatgga aggatttgaa
    13981 atctgcggcc tggtgagcac cggtaaaagc aaggaattgc tgaatcaaaa acttggaggg
    14041 ggttatgccc ttttttcaga ttattcaaaa gccctggaat tgacccgtcc ggatgcggtc
    14101 agcatcaata cttatcccga tacgcatgaa tcctatgcac tgcaggcctt tgaccagggt
    14161 tgccatgtct ttatggaaaa accgatcgcg gatacggtgg ccggagcgga gcgggtggcc
    14221 gaagctgccc ggcaagccgg caagaaactg gtgatcgggt atatcctgcg ccatcaccct
    14281 tcctggataa agtttattga aatcgcccaa caaatgggca aacccctggt gatgcgcatg
    14341 aacctcaacc agcaaagcca tggttatatg tggacggtgc accgcaacct gatgaaaagc
    14401 ctgagcccca tcgtggattg cggggtccat tacatcgatg tgatgtgcca gatggtccgc
    14461 tccaaaccca tccaggttgg cgccatcgga gcgaggctga ccgaagccat accggcaggg
    14521 aattataatt atggacaatt gcagatccgt tttgaggatg gatccgtagg ttggtatgaa
    14581 gccggatggg ggcccatggt gagcgaaacc gctttttttg taaaagatgt gttcgggccc
    14641 aagggtgctg tttccatcgt agcccgcgat gccggcggtt ccggcaaatc ttcatccatt
    14701 gacgcccata ccaaaacgga atccctgcgc atccatcacg cggaactcaa cgaacaggac
    14761 gagtttgtcc ggccggatga atggctggac atgactgatg aacccggcca tcaggaactt
    14821 tgcaaccgcg aacaggccta tttcctcaaa gcaatccggg aagacctcga tctcacccac
    14881 catgtacagg acgccgtgga tagtctgcgc attgcttttg cctgcgatga atcggtaagg
    14941 acgggacagg tagttaagtt gtagaattgt agaattgttg aattgtttag tgactgctga
    15001 ctactgacta ctgactactg actactgact actgactact gactactgac tattgactat
    15061 tgacaaacac cggtgcgagt tgtgatcggt aagtggtatc cgtcagttgt tttacttctt
    15121 tggtttgcca attcattaga aggatatggt attgcctgcc attggggtca gaggagtcat
    15181 aagtcagcca tttgccatcg gggctccagt catgccatcc ttcccctttt gggccggtgg
    15241 tgattttcca ttgtttggag ccctccgggt ttaccgcaca gatctggtga cgtccatcct
    15301 gcatggagat gtagctgatg aaatgctcgg tgggatgcca acggggcgct ccggccttgt
    15361 aacccggttc tttcgctgac acattgtctt ccggataatg ggtcagttgc ctgctgccgg
    15421 acccatccga attcatgata aaaagttctt catggctgtt ccggtctctt ttgttctgtt
    15481 tataggcata aacgatctga gaaccatccg gggaaaaaca ggggtctccg taacgggcgg
    15541 cggtgtcgga ggtgatttgg gagtattgcc cggatttggt gttcacgata aaaagctgaa
    15601 accgcgtcgt gggtcccagg cgcccggtga ctaccatttc ttccccgtga tgccggctgc
    15661 tcatccagct gtcttcaagg cggaggtcgc tgactttccg gatggactgg ccgccggggt
    15721 cggtttcata gagaaaaaaa caacggtaac aggtatcccg gtcgctgatg aaatacaatt
    15781 tgtcctgaaa ggcataatag gtccaggcga catcgtcatt ccgggtcagg tttcttttac
    15841 cgctgccgtc catattcatg agcatgactt cccagtcgtc tttggtggta tccggaatgt
    15901 agacgttata agcgatgaaa tagcctttct ccgaacccgc ggcttgaccg gaagggctgg
    15961 aatcccggca ggaaatcaaa agaaacaaac aggcattcaa ataaaggaaa tttttcatca
    16021 ctgggttttg aggaattcca aaggctccgg gacgggattg tccgggaact ggaatttcgt
    16081 tgactgcaaa gaaacttaaa agcaatggcc tgccattgga aaaaatttac ctattccagg
    16141 gccacataat tggtaaaagc cctttgctgt tctacaaact gggcgggggt gaaccctgag
    16201 taatttttaa attcatgaat gaagtgggcc tggtcgtaat acccggaagc cgcagcaatc
    16261 gcggtccagc ctccggcagc cggcgcgtcg atacgtgaaa tggctttctg aaaacggatg
    16321 atctttaaaa attcttttgg actgagtccg gtgtgatccc ggaaaaggcg gatcaggtgt
    16381 ttgtgggaat aacctatttt gacggacagg ttggcaatgg tggtttgggc cggatggagc
    16441 aggatctgct ccaccgcata gtccacgcag ggattcaggc ggaggtccgg accgtattgc
    16501 gccaagagta gtttttcggc gagggcaaat ttttcccagg cccccggaag gggcaatagg
    16561 gcttcacgga cccgcaggat gtccttccgg aaagagtgct cgccatccac gaccgcatcg
    16621 gtgtattcac cgaggggacc
//
LOCUS       DAOQXT010000105        15507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4258_length_15507_cov_5.789154, whole genome shotgun sequence.
ACCESSION   DAOQXT010000105 DAOQXT010000000
VERSION     DAOQXT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15507
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4258_length_15507_cov_5.789154"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..556
                     /gene="merTP"
                     /locus_tag="PKM27_15560"
     CDS             2..556
                     /gene="merTP"
                     /locus_tag="PKM27_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerTP"
                     /protein_id="HNR08736.1"
                     /translation="MAASLSCITPVLALVAGTSGLASTFSWLEPARPYFIGLTILVLG
                     FAWYQKLKPKKQIDCNCETDEKPKFTQSKMFLGIVTVFAGLMLAFPMYAHIFYSKTEK
                     QVIVVDKTNIQTVEFTIIGMTCASCEEHVNHEVNKLTGIIKSTASYESGNAIIEFDNS
                     KTSTAEIEKAIKSTGYSVTDKKEK"
     gene            556..762
                     /locus_tag="PKM27_15565"
     CDS             556..762
                     /locus_tag="PKM27_15565"
                     /inference="COORDINATES: protein motif:HMM:NF041374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HNR08737.1"
                     /translation="MEIILQSTITCPNCRHKKEETMPTDACQYFYECENCKTVLKPKQ
                     GDCCVYCSYGSVKCPPIQQDKKCC"
     gene            1302..1865
                     /locus_tag="PKM27_15570"
     CDS             1302..1865
                     /locus_tag="PKM27_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNR08738.1"
                     /translation="MNIDNILDNIYILPQTSKQTLKKHIDEVGFSKGHILLRADKIEN
                     NIYFIKQGIVRGYSKTTDNEITFWFGKEGDTIISMKSYVSNQKGYEDIELLESCKLYE
                     LKTKSLKKLFDEDIHIANWGRRFAEQELLKTEERLISRQFRTAKERYKEILKDNPDLI
                     QRVQLGHIASYLGITQVSLSRIRAEIK"
     gene            1942..2271
                     /locus_tag="PKM27_15575"
     CDS             1942..2271
                     /locus_tag="PKM27_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNR08739.1"
                     /translation="MNWIILVIAGLFEVAFAFCLGKAKGTTGNEMYLWYGGFLVTLAI
                     SMGLLVKATQTLPIGTAYAVWTGIGAVGTVLVGIFVFKEPATFLRILFLTTLIGSIVG
                     LKAVSVH"
     gene            2290..2748
                     /locus_tag="PKM27_15580"
     CDS             2290..2748
                     /locus_tag="PKM27_15580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08740.1"
                     /translation="MNITIRKAEVEDSEQLWRLMKELAIFEKYINSFAITPEIVRNSG
                     FKKKPPDFHCIVAENNSKIAGMLVYYYLPYTAQNKPAIYMKELYVDENYRGQKIGEQL
                     MTVLKNEAKENNCSQIKWTVASWNEAGQRFYERLGANENKEWLNYEWNIQ"
     gene            2758..3291
                     /locus_tag="PKM27_15585"
     CDS             2758..3291
                     /locus_tag="PKM27_15585"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08741.1"
                     /translation="MENISVQVQKVTLNDIQQLQEIGRKTFYETFAGSNTKEDMQKYL
                     DESFSETKLTEELTDQNSEFYFAKIEDKIIGYLKVNFGASQTELKDNKALEIERIYVP
                     KEFHGKKVGQILYDKAIEIAEQHNSDYVWLGVWEENPRAINFYKKNGFVEFDKHIFKL
                     GNDEQTDIMMKLKLNNN"
     gene            3546..3728
                     /locus_tag="PKM27_15590"
     CDS             3546..3728
                     /locus_tag="PKM27_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08742.1"
                     /translation="METKSTDRKSRNSIAIWIGLGIALGAAYGAYSGNTSLGVGIGLA
                     VGVVVGAIFSAVKNGK"
     gene            complement(4100..4888)
                     /locus_tag="PKM27_15595"
     CDS             complement(4100..4888)
                     /locus_tag="PKM27_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNR08743.1"
                     /translation="MESNYSYLQVEITNGILIARIDRPKALNALNTQVFAELGNLISV
                     FAPGIQGLKGIVITGSGDRAFAAGADISEFTGIGTGKAKEFLTPGNVVLRQIETFHRP
                     VIAAVNGFALGGGCELAMACHLRIAGEKARFGLPEASLGILPGYGGTQRLPQLIGKSK
                     ALEMMLTGDMITAEEAFRLGLVNHLTEAGKETEKAIEIIEKISSRGPLSIQRIIAAVN
                     TYYPHLEAGIDEEGNHFRDLADTADFREGVAAFLGKRKAVFEGK"
     gene            5060..5839
                     /locus_tag="PKM27_15600"
     CDS             5060..5839
                     /locus_tag="PKM27_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR08744.1"
                     /translation="MAKIITIANQKGGVGKTTTAINLAASLAILEKKVLLVDADPQAN
                     AGSGVGVQVDDLALSLYDCMINGADPDAVILQTETPNLWILPSHPDLVGAEIEMVYKD
                     RREFILKNILDKISHRYDFILIDCLPSLGLITINALVAAHSVLIPIQCEIFALEGLSR
                     LKRTISIVRENLNPGLEIEGIVLSMYDQRLRLANLVIHELRSNAQDYIFNTIIHRNSK
                     IGEAPSVHKPVLLYDAAGKGTINFLNLATELLQRQQAPVGV"
     gene            5849..6712
                     /locus_tag="PKM27_15605"
     CDS             5849..6712
                     /locus_tag="PKM27_15605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNR08745.1"
                     /translation="MDQKKELGKGIRALLGNMENQSKVAALSNVMNVPLAFIETNPFQ
                     PRNEFDQAGLEELASTIRIHGLIQPVTVRKLTDQRYQLISGERRVRAARMAGLTEVPA
                     FIRLADDQGMLEMALIENIHRENLNPLEVAISMSRLIEECKLTHEDMSTRVGKDRSTV
                     TNYIRLLKLPPDIQQAVKSKKLSMGHARALAGVDNLVLQMKLFKECLLHEWSVRQLEF
                     TIRSYQSAGKPKAAAVPPSPGGLLKTYQEKANALFGRKIQIKSDKKGKGSLIVPFASL
                     DELDEMLDRLS"
     gene            6777..7379
                     /locus_tag="PKM27_15610"
     CDS             6777..7379
                     /locus_tag="PKM27_15610"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNR08746.1"
                     /translation="MNKEIRHIITTIKTGFPPAGNILIIGLLLLLGFTGQAQQDTLPA
                     APLNVDSLSLVQDKRPRTALWLGLALPGAGQVYNKRWWKVPLVYGAYGFAVYRIRETR
                     NRYLEFDGYFKEALATNAKVEVVPDVFFDARQIKTYRDRFRDDKDRAVFLLIGIHFLS
                     ALEAFVDAHLKNFNIDDDLGLQLVNPDSGGPAIGLRYRLH"
     gene            complement(7371..7688)
                     /locus_tag="PKM27_15615"
     CDS             complement(7371..7688)
                     /locus_tag="PKM27_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNR08747.1"
                     /translation="MDATSFLKDLPGKIKPEMLEGMETLFHFDLEGEGGGQYTVKVKD
                     GKLEVNPGLLEEPKCVVKSSTETLMGIVEGKINPMMAVMTGKIKISNPGEMIKYAKVF
                     GLM"
     gene            7875..8798
                     /gene="fmt"
                     /locus_tag="PKM27_15620"
     CDS             7875..8798
                     /gene="fmt"
                     /locus_tag="PKM27_15620"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNR08748.1"
                     /translation="MRIVFMGTPAFAVPSLEALLCSHHRVVGVVTAPDSLGGRGGRQI
                     IQSEVKTCAQAHKLHIMQPPKLKAPEFFEELKSLQADIQVVVAFRMLPEKIWSMPPLG
                     TINVHGSLLPKYRGAAPIHWAVINGEEFTGVTVFRLRHEIDSGEILKQHAVPIGPDET
                     TGDVYDKLKTAGAATLLEVLADMERGDSHPIPQDPSLVSLAPKLYHTDGILDFSKPRR
                     ELHNLIRGLSPTPTAWFVFNGLKFLLYRSHLPEVSPPAREAGNLQILGKKLYLHATDG
                     AIEITEIQQENKKRMAAADFINGMKNHPMFN"
     gene            complement(8805..10208)
                     /locus_tag="PKM27_15625"
     CDS             complement(8805..10208)
                     /locus_tag="PKM27_15625"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HNR08749.1"
                     /translation="MLGLLLFLLSFLSASGKGYDIRVELKNAPSDTLLLGYYYAERQY
                     VRDTAIGKNGLFHFQGDSALAPGIYILVMPPKYDFIQLFINEDEQQLEVQADALNTIS
                     SIKVKGSEDNTLFYDYLRFLELKRPQAQGLQEKLKAEKEAGRATDALQQQLDKINQEV
                     KSRQVELTQKYPGTITAMLIRATFDVEVPEMTGTPEEVEQKKYLWYKSHYFDHLALSD
                     SRLLRSPLIHDRINYFVEKLTLQHPDSVKSSLDEILRRVRPAEETFKYYLIHFVNHYA
                     ASKIVGFDGIYVHLVDEYYAKGFAPWVGEEQLTKMKQNAAALRPILLGKTAPEIKLIR
                     HPEGKPVSLHGIRSPYTILFIWDPECSHCKASLPHVIDFYNKYKDKGVEILAVCSQFG
                     DKVPACWKYLEEQKMNPGWINAADPQHTSRFKILYDVKTTPQIFILDADKKILSKNIG
                     AEQLPEVMDHILAVPKP"
     gene            10357..11505
                     /locus_tag="PKM27_15630"
     CDS             10357..11505
                     /locus_tag="PKM27_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR08750.1"
                     /translation="MSHIINRLGEERENYFNAISPPVMQTSNYAFESVMDFRKKISTE
                     FESHVYTRGNNPTVEILRKKLAALEHTEDCLVFGSGVAAVAAAVMANISQGEHIVCVR
                     SAYSWTKTLLQKYLPRFGVSHSFVDGRYIDHIEAAILPNTRILYLESPNSMIFELQDL
                     EACAALAKKYGLVTILDNSYCSPIFQQPADFGIDLVLHSGTKYINGHSDVVMGVLCGK
                     REMIKKIFYSEFMNIGHILSPHDAFLAIRGLRTLPLRVQRSHETCAKLMEALQNHPKV
                     EKIHHPFIDEQQALAVRQMKGGGMFTLALKAARKEQVIQFCDSLENFLIAVSWGGHES
                     LIMPVVTFYDIPGQPDPELPWNLVRFYIGLEDPHYLLADILQALDRIS"
     gene            11571..13769
                     /locus_tag="PKM27_15635"
     CDS             11571..13769
                     /locus_tag="PKM27_15635"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNR08751.1"
                     /translation="MKSGCLLFLILLSFRASGADTLDLSRPFIIQAGQCREKMTIDGA
                     LDESIWQNAPKASAFQEHWPQNARVPLHKTEAMIASDDHSIYVAFTCYDTSKNFIIQT
                     LKRDFQLDGNDAVAVILDPTGQKNFAYAFLLSPAGVQGEALINQFEADANWDSKWFSA
                     VKIYEDRWTAELAIPLHILRYDPNLLVWNVNFLRGDVKNFRFYSWTKIPQQFDIFNLG
                     YLGSLVWPEPLPKKGGQITLIPYLNANTSREFEEHTVQKLNFGFGGDAKITLSSSLNL
                     DLTANPDFSQVEVDRQVTNLTRFNVFFPERRNFFLENADVFSQFGDPGNPIFFSRRIG
                     LDNDGNAVPILGGVRLSGNMGETWRIGALSMQTKGTAEQAGQNYSALTLHKKVLKRSL
                     IKGLVTNRQAVDGIVVLPKDYGRNASLEMIYQNEKGSLFNWLGLHKSFKHDLKDKNGQ
                     LSAGVWYNDKNFSTVTGIGSTGTNFTADMGFVNQIDNYDSERDTVIRQGFNSAFSFNQ
                     YILRTPDHKFINQHQFSLNNSFKFLPGETSLLDWSTSLEYELSFKSSSEIEWSVRRNQ
                     LQLLYPFSFTDGSPLPPGKYSYANVNAEYRSDLRKRFGFTMSASAGSFYNGSLTAFTA
                     GMLYRAQPWGNFEVNFEFNDLDFPDPFGRDQLILINPRIEFNFTKNLFWTTFLQYNTQ
                     ADNFNINSRFQWRYKPMSDIYMVYTDNYAVKFWGPKNRSFVLKINYWLNI"
     gene            13816..14253
                     /locus_tag="PKM27_15640"
     CDS             13816..14253
                     /locus_tag="PKM27_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08752.1"
                     /translation="MLKGLVLLFLFIAQPIRAQTAFIQDEKFGRVWIKPEHPASFTRT
                     DQERNLQEYFTKFYSGHGDLDSGFKALVLLTLIIDGEGFANLSKATASNPAVSLETLQ
                     IAKSIHEMPRWFPAEERDKKVPFQVLLSLQFEGNKVLTRYLKF"
     gene            complement(14259..14780)
                     /locus_tag="PKM27_15645"
     CDS             complement(14259..14780)
                     /locus_tag="PKM27_15645"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HNR08753.1"
                     /translation="MNEIITHRPKKELQADHAWRLFTSLQEMIQFADYKVYLLYMIAG
                     IVLSIIFSEYKELATLNPVFKACFAMLLLVSGLLIYFSMKTMMPRSHNDHHTQTKKLI
                     YFMDIASQQDKDFVGRFLELPTDQITEDLLNQAAILSDILKQKFLNLKRAMWAFYGIL
                     FIVLVMQILKIAM"
     gene            14898..15482
                     /locus_tag="PKM27_15650"
     CDS             14898..15482
                     /locus_tag="PKM27_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNR08754.1"
                     /translation="MVSWSVTDFLTVSFTLFAVIDVIGSVPLLISLKSKMGGIEEFRA
                     TFLSGGLMILFLFVGEKLLNLMGVDLRSFAVAGSIVIFILGLELVLGIEFFKTDAQVQ
                     SGTLVPIAFPLIAGSGTLTTVISLKANYSTTVILTAILVNLAIVYLVLKSLNLIERAL
                     GPGGLLAVRKFFGVILLAIAVKIFAANLPGLLPG"
BASE COUNT         4226 a         3731 c         3631 g         3919 t
ORIGIN      
        1 aattgcagct tcactttctt gcattacacc tgttttagca cttgtagcag ggacaagcgg
       61 acttgcttct actttttctt ggcttgagcc ggcaagaccc tacttcatcg gcttgacaat
      121 tttagtgctt ggtttcgctt ggtatcaaaa actaaaacca aaaaaacaaa ttgactgcaa
      181 ttgtgaaacg gacgagaaac cgaaattcac acaaagcaaa atgtttctcg gtattgtaac
      241 agttttcgca ggacttatgc ttgcctttcc aatgtatgct catattttct attcaaagac
      301 agagaagcaa gtaattgttg ttgacaaaac aaatattcag acagttgagt ttaccattat
      361 cggaatgact tgtgcaagtt gcgaagaaca cgtaaaccac gaagtgaata agttaacggg
      421 cataatcaaa tcaacagcgt cctatgaaag tggaaacgct atcatagagt ttgacaattc
      481 aaaaacttca actgccgaaa ttgagaaagc gattaaatca acaggttatt cagtaacaga
      541 caaaaaagag aaataatgga aatcatatta caatcaacaa ttacttgccc aaactgcaga
      601 cataagaaag aagaaactat gccgactgat gcctgccaat atttttacga atgtgaaaat
      661 tgcaagaccg ttctgaaacc aaaacaagga gattgttgtg tttattgtag ttacggaagt
      721 gtaaaatgtc cgccaataca acaagacaag aaatgttgct aacgacagaa agcaaagaag
      781 cccagccact aacagcggtt tggcgtaatg gcgggttcag tgcttcggtt gacagttttg
      841 tgggtcaaca aacagttgtg cttcgtatca acatctgtgg taaaaatccg ccactacgcc
      901 aagccgcaaa acgttatgcg agattgtcga aactactcta attccgcttc gtaatctttc
      961 tcttcgcccg tcatttcctt cccgttgaga tctgccgtcc cggtggctca atcttccatc
     1021 gcttattgag ggcatcccat gcccggcggt gcgctccgag gaaagtgtat ttgtcgtggg
     1081 actcaacatt tgcgtttttc atagcaacaa atcaggacgg ggaatatttc taaatatggc
     1141 aaccccgcat aacacgagct tagcgacatg gccgctgagt agttggtatt gataattctg
     1201 ctccgaaatt gcaatttagt cgttctacac gcagcaaccg atataacggc cacggcgcca
     1261 agctctggtc cgttataggc aaccttaccg aaacccctca aatgaatata gacaacattc
     1321 ttgacaacat atatatattg ccacaaacat ctaagcagac gcttaaaaag catattgatg
     1381 aagtgggttt ttcaaaggga catatcttgt taagagcaga caaaatcgaa aacaatatct
     1441 actttattaa acaaggaatt gtcagaggtt attctaaaac aaccgacaac gaaataactt
     1501 tttggtttgg aaaagaggga gatacaatta tttctatgaa aagttatgtt tcaaatcaga
     1561 aaggatatga agatattgaa ttgttggaaa gttgcaaact gtatgaactg aaaacaaaaa
     1621 gccttaaaaa actttttgac gaagacattc atattgcaaa ttgggggcga agatttgccg
     1681 aacaggagct tttaaaaaca gaagaacgtc ttatttcccg acaattccga actgcaaaag
     1741 aacgttacaa agaaattcta aaagataatc ccgacctaat tcaacgggtt caattaggac
     1801 acatagcttc atatttggga ataacacaag ttagtttaag cagaataaga gcagaaataa
     1861 aatagctagt tcatttttta tcatttgtaa aattttcttt caatttcatt tccgaatttt
     1921 gaaagcaaaa aattagagaa aatgaattgg attattttag tgattgctgg tttatttgaa
     1981 gtcgcttttg ccttttgttt aggtaaagca aaaggaacaa caggaaacga aatgtattta
     2041 tggtatggag ggtttttagt tactcttgcc atcagtatgg ggcttcttgt taaagctact
     2101 caaacattac caatcggaac agcttatgcc gtctggacag gtatcggagc cgtaggaaca
     2161 gttttagtag ggatttttgt atttaaagaa cccgcaacct tcttacgaat actgtttctg
     2221 actactttaa ttggctcaat cgttgggctt aaagctgttt cggtacatta aataaattaa
     2281 atttacaaaa tgaatatcac aatacgaaaa gcagaagtag aagacagtga acaactttgg
     2341 cggttaatga aagaattggc aatttttgaa aagtatatta attcatttgc cattacaccg
     2401 gaaattgtca gaaatagtgg ttttaaaaaa aaaccacctg attttcattg tattgttgcg
     2461 gaaaacaata gtaaaattgc aggaatgttg gtgtattatt atcttcctta cacagcccag
     2521 aacaaacctg ctatttatat gaaagaattg tatgtagatg aaaattacag agggcaaaaa
     2581 atcggagagc agctaatgac tgttttaaaa aatgaagcaa aagaaaataa ttgttcacag
     2641 attaaatgga cagttgcatc ttggaatgaa gcaggacaaa ggttttacga gagattagga
     2701 gcaaatgaga ataaagaatg gttgaattat gaatggaata ttcaataaaa taaaacaatg
     2761 gaaaatatat cagtacaagt acagaaagtt acgctaaatg acattcaaca attacaggaa
     2821 ataggaagaa aaacttttta tgaaactttt gcaggttcaa acactaaaga agatatgcaa
     2881 aaatacttag acgaaagttt tagcgaaacc aaattgacag aagaactcac agaccaaaac
     2941 tctgaatttt attttgcaaa aattgaagac aaaataatcg gctacttaaa agtaaatttc
     3001 ggagcatctc aaacagaatt aaaagacaac aaagcgcttg aaattgaaag gatttacgtt
     3061 ccaaaagaat ttcacggaaa gaaagttgga caaattcttt atgataaagc aattgaaatt
     3121 gcagaacaac acaattctga ctatgtttgg ttaggcgttt gggaagaaaa cccacgagca
     3181 ataaattttt acaagaaaaa tggtttcgta gaatttgaca aacatatttt caagttaggc
     3241 aatgacgaac aaacggacat aatgatgaaa ctaaaactta ataacaacta aaaaggctgc
     3301 ctataacaag ggtttggcga aatggcgggt taagtgctaa attcaacgtc agtaattcta
     3361 tcaccgcata agccattttt atttcctttt ttgtaattta gcaaatagaa aatggcttat
     3421 gctactgttg tgctaaatcg aaagctcgta cttctatttt cgccacttcg caaagccccg
     3481 aaccgttggc ggtcattgtc cgaggactca ttagaatcga tggtataatt cttaaacaaa
     3541 aaaatatgga aacaaaatcg acagatagaa aatcaagaaa tagtatagct atttggattg
     3601 gtcttggaat cgcattgggg gccgcctacg gtgcttattc gggaaatacc tcattaggcg
     3661 tagggattgg actagctgtg ggtgttgttg ttggtgcaat atttagcgct gtaaagaacg
     3721 gaaagtaaaa tttcaatcaa cactataacc agtaacatct agtttggacg tccaatataa
     3781 aggacaagag ggtttgaaaa atgacgattg taggattatg agacttgttt tgtaatgaca
     3841 tattacttgg tttacgctga ctacggaaca acgaaccgcc aacacaatgt atgtgccatt
     3901 gcggggatta aggtaaagtt gtagcttccg tttcaaataa cgttcggtct cgtgggacag
     3961 tttcggagta gtcgggcaaa tcagtcgtgc ctccttcatt tgccctccag gcccgcaacg
     4021 tcacatacac caacgttacc ggtcatgtca taaccaaaaa ctttgagaca atttaaccca
     4081 tttttgacgg tatgctcccc tatttccctt caaaaaccgc cttcctcttt cccaaaaaag
     4141 ccgctacccc ttccctgaaa tccgccgtgt cggccagatc gcggaaatga ttgccctctt
     4201 catcaatgcc ggcttccagg tgcggataat aagtgtttac tgcggcgatg atcctttgaa
     4261 tggacaaggg gccccggctg cttatcttct cgataatctc gatcgctttt tcagtctctt
     4321 ttcccgcttc ggtcaggtgg ttgaccaggc ccaggcggaa agcttcttcg gcggtgatca
     4381 tatccccggt gagcatcatt tccagggctt ttgatttgcc gatcagttga gggagccgct
     4441 gggtgcctcc gtaaccgggc aggatgccga gactggcttc gggcaagccg aaccgggcct
     4501 tttcacccgc gatgcgaaga tgacaagcca tggccagttc acatcccccg cccagggcaa
     4561 aaccattcac ggcagcaatc accggacggt gaaaggtctc gatctgcctc agcaccacgt
     4621 tgccgggagt gaggaattct ttggcttttc cagtgcctat cccggtgaat tcactgatat
     4681 cggcaccagc ggcaaaagcc ctgtccccag aaccggtgat gactatacct ttcaggccct
     4741 ggatacctgg tgcaaagact gagatcagat ttcccaattc ggcaaagact tgggtattga
     4801 gggcattcaa ggccttaggc cggtcgatcc tggcgatgag gataccgttc gtgatttcca
     4861 cctggaggta agagtagttg gattccatgg ctttatgaat tgataattag aattacgaac
     4921 ataatgattc tgaggatagg aagggagttt ggatcaggga aggattgtgg gacaggctga
     4981 gaggataccc ccatcccccc tgccctcctt aaactaagac gcaaaaaatg taattttgta
     5041 aacgttcagg aatccggaca tggcaaaaat catcaccata gcgaaccaaa aaggcggggt
     5101 gggtaagacc accacggcca tcaacctggc cgccagcctg gccatcctgg aaaaaaaagt
     5161 gctgctcgtg gatgccgatc cccaggccaa tgccggctca ggagtgggcg tccaggtgga
     5221 tgatctggcc ctctccctct acgactgtat gatcaacggc gccgacccgg acgctgtgat
     5281 cctgcaaacc gaaaccccca acctctggat cctgccctcc catcccgacc tggtaggcgc
     5341 ggaaatcgaa atggtgtaca aggaccggcg ggagttcata ttaaaaaaca tcctggataa
     5401 aatcagccac cgctacgatt ttattctgat cgattgcctg ccttccctgg gcctcatcac
     5461 cataaacgcc ctcgtcgccg cgcattccgt actgatcccc attcaatgtg agatcttcgc
     5521 cctggagggc ctcagccggc tcaaacggac catttccata gtcagggaaa acctgaaccc
     5581 gggcctggaa atcgaaggca tcgtcctgtc gatgtacgat cagcgcctgc gcctcgccaa
     5641 cctggtcatt catgaactgc gcagcaatgc acaggactat atcttcaaca ccatcatcca
     5701 ccggaattcc aagatcggcg aagcgcccag cgtgcacaaa ccggtcttgt tgtacgatgc
     5761 cgcgggaaaa gggaccatca attttctgaa cctggccacg gaattgctgc aaaggcaaca
     5821 ggcccccgta ggagtataat ataaagctat ggaccaaaaa aaagaattag gcaaaggcat
     5881 ccgggcgctg ctgggcaata tggaaaacca gtcaaaagtg gccgcgctgt ccaacgtgat
     5941 gaatgtaccc ctggccttta tcgaaaccaa tcccttccag cccaggaatg aatttgacca
     6001 ggcaggcctg gaagaactcg cctccaccat ccggatccac ggactcatcc agccggtcac
     6061 ggtccggaaa ctgaccgacc agaggtacca gctcatctcc ggggagcgga gggtaagggc
     6121 cgcccgcatg gccggcctca cggaagttcc ggctttcatc cggctggcgg atgaccaggg
     6181 catgctcgaa atggccctga ttgaaaacat ccaccgggaa aacctcaatc cgctggaagt
     6241 ggccatttcc atgtcccgcc tcattgaaga atgcaaactc actcatgaag atatgtccac
     6301 ccgggtgggt aaagaccgga gcacggtgac caattatatc cgtctgctca aactgcctcc
     6361 ggacatccag caagcggtga aatcgaaaaa actgagcatg ggccatgccc gcgccctggc
     6421 cggcgtggac aacctggtct tgcaaatgaa attgtttaaa gaatgccttc tgcatgaatg
     6481 gtcggtgagg caactcgagt ttacgatccg ctcctaccag tccgccggca aacccaaagc
     6541 cgccgctgtt cctccttccc caggcggcct gcttaaaact tatcaggaaa aggccaatgc
     6601 cctttttggc cgtaaaatcc agattaaatc cgataaaaaa ggcaaaggca gcctgattgt
     6661 tccttttgcc agcctggatg aactggatga aatgctcgac cgtttgtcct gaacccgcgg
     6721 acctcaacct acatttacca tggatctatc gttataccca tcctggaacc caacgtttga
     6781 ataaagaaat ccggcatatc attacaacca taaaaacagg cttccctccc gcggggaata
     6841 tccttatcat aggcttgctg ctgctccttg gttttacagg gcaggctcag caggatactt
     6901 tgccggcggc cccgctcaac gtcgacagcc tcagcctcgt gcaggataaa cgaccccgga
     6961 cggcgctctg gctgggcctg gccctgcccg gtgcgggaca ggtttacaat aaaaggtggt
     7021 ggaaggtgcc cctggtgtat ggggcttatg gttttgcggt gtaccggatc cgggaaacga
     7081 gaaaccggta tttggaattt gacggatatt ttaaagaggc cctcgctacc aatgccaagg
     7141 tggaagtggt gccggacgtg ttttttgatg cccggcaaat taaaacttac cgggaccggt
     7201 tccgcgacga caaggaccgt gccgtctttc tgctgatcgg tatccatttt ttatccgccc
     7261 tggaagcctt tgtggacgcc cacctcaaga attttaacat cgacgacgat ctcgggttgc
     7321 aactggtcaa tccggattcg ggcggacccg caattggcct tagataccgg ctacattagt
     7381 ccgaagacct tggcgtattt gatcatttct cccgggttgc tgattttgat cttgcccgtc
     7441 atcacggcca tcatcggatt gattttgccc tcgacgatgc ccatcagggt ttccgtgctc
     7501 gattttacca cgcatttggg ttcctcgagg agtcccggat tcacctccag cttgccgtcc
     7561 ttcactttta ccgtgtactg gccgccgccc tccccttcga gatcaaaatg aaagagggtc
     7621 tccatgcctt ccagcatttc gggtttgatt ttaccgggaa ggtctttcag aaaagatgtt
     7681 gcatccatga attatatttt accggtaaga taatcgcgaa ttatgaatta ctccacgaaa
     7741 cttcggtata cagaatatga ttgtcaaact tgccaactag ctaactgact aactaaataa
     7801 ctagcaaact gaataactag caaactgact aactagctaa ctgactaact agctaactga
     7861 ctaactttac tcccatgcgt atcgtcttca tgggtacccc ggcttttgcc gtcccttcac
     7921 tggaagcctt gctctgctct catcaccgcg tcgtgggcgt ggtgacggct ccggattccc
     7981 tcggaggccg gggaggacgc caaatcatcc aatccgaagt aaaaacttgt gcgcaggccc
     8041 acaagctgca catcatgcag cctccaaaac tgaaggcccc tgaatttttt gaagaattga
     8101 agtccctgca ggcggacata caggtcgtcg tcgcttttcg catgcttccc gagaagatct
     8161 ggagcatgcc acccctggga accatcaatg tgcatggctc tttattacca aaataccgcg
     8221 gggccgcccc catacattgg gcggtgatca acggcgagga atttacaggg gtcaccgttt
     8281 tcaggctcag gcatgaaatt gactccgggg aaatactcaa acagcatgct gtgcctattg
     8341 gccccgatga aacgaccggc gatgtgtacg acaaactcaa aacagcgggc gccgccacct
     8401 tgctggaggt gctggcggat atggaacggg gcgactccca ccctataccc caggatccat
     8461 ccctggtcag cctggctccc aaactttacc atacggatgg cattcttgat ttttcaaaac
     8521 caaggaggga attgcacaat ttaatccggg ggctcagccc caccccaacc gcctggttcg
     8581 tttttaatgg attaaaattc ctcttgtacc ggtcgcacct gcccgaggtt tcaccgccgg
     8641 caagggaagc gggaaacctg cagattcttg gaaaaaaact ttatttacat gcgacggacg
     8701 gagccattga gatcaccgag atccagcagg aaaacaaaaa gaggatggcc gccgccgatt
     8761 ttataaacgg catgaaaaac catccgatgt ttaattgacc gggcctatgg ttttggaacc
     8821 gcgaggatat gatccatgac ctcgggaagt tgttccgccc cgatattttt tgagaggatt
     8881 tttttatccg catccaggat gaagatctgg ggtgtggttt tgacatcgta caggatcttg
     8941 aaccgggagg tatgctgggg gtcggccgca ttgatccagc ccggattcat tttttgctct
     9001 tccaggtatt tccagcaggc ggggacctta tccccgaact ggctgcagac ggccaggatt
     9061 tccactcctt tgtctttgta tttgttataa aaatcgatca catggggcag ggaggccttg
     9121 caatggctgc attcgggatc ccagataaac aggatggtgt agggagaccg gatgccgtga
     9181 aggcttaccg gtttaccttc cgggtgcctg atgagtttga tttccggggc ggttttaccg
     9241 agcaggatgg gccggagggc agcggcattt tgtttcattt tggtcagttg ttcctccccg
     9301 acccatggcg caaaaccttt ggcataatat tcatccacca ggtgaacata gattccgtcg
     9361 aagcctacga ttttagaggc cgcgtaatgg tttacaaaat gaatgaggta atatttgaag
     9421 gtttcttcgg caggccggac ccggcgtaga atttcatcca gtgaagattt gaccgaatcg
     9481 ggatgctgca gggtcagttt ttctacaaaa taattgatcc ggtcatggat taacggactc
     9541 cggagcaggc gggaatcgga caaagccagg tggtcaaaat aatgcgactt ataccagaga
     9601 tattttttct gttccacctc ctccggggtt ccggtcatct ccggaacctc cacatcaaaa
     9661 gttgcccgga tcagcatagc ggtgatcgtc cccggatatt tttgggtgag ttccacctgc
     9721 ctgcttttga cttcctggtt tattttatcc aattgttgct gaagggcatc ggtagctctt
     9781 ccggcttctt tctccgcctt cagtttttcc tgtaatccct gggcctgcgg ccttttcaac
     9841 tccaggaaac gcaggtaatc gtaaaacaag gtattgtcct cggacccctt gacctttata
     9901 ctggatatgg tattcagggc atccgcctgc acctccagct gctgttcgtc ttcattgata
     9961 aaaagctgga taaaatcgta tttcggcggc atcaccagga tatagatacc gggcgcgagc
    10021 gcactgtcgc cctggaaatg aaagagtccg tttttaccta tggccgtatc cctgacatac
    10081 tggcgttcgg cataataata tccgagcaag agggtgtcgg aaggggcatt ttttaattcg
    10141 acccggatgt cgtatccttt accggaagcg gacaaaaagg aaaggagaaa gaggagaagt
    10201 cccagcataa tggcaataaa aacacaaaaa tcgaattatc cggccatatc cctggcttta
    10261 ttaatggttt tttaaccaag agctgaggaa cagggtcccg tcgaagcgaa ttggcggtat
    10321 ttattaagcg tccttcaata cctttgattt atggaaatga gtcatattat aaaccgcctg
    10381 ggtgaagaac gggaaaacta ttttaatgcc atatcccctc cggtcatgca gaccagcaat
    10441 tatgcctttg aatcggtgat ggatttcaga aaaaaaatct ccaccgaatt cgaatcccat
    10501 gtctataccc ggggcaacaa ccctacggtg gaaatattga ggaaaaaact ggccgccctg
    10561 gaacataccg aagattgcct ggtgtttggg agcggggtgg ctgccgtcgc cgccgcggtc
    10621 atggccaata tcagccaggg ggagcatatc gtctgcgtcc gttcggctta ctcctggacc
    10681 aaaaccctcc tgcaaaaata cctgccgagg tttggggtaa gccacagttt tgtggacggc
    10741 cgctacatcg accacataga agccgctatc ctgcccaata cccgcatcct ctacctggaa
    10801 agccccaaca gcatgatctt cgaactgcag gacctggagg cctgtgcggc gctggctaaa
    10861 aaatacggct tggtcacgat cctggacaac agttattgct cgccgatatt ccaacagccc
    10921 gctgatttcg ggatcgacct ggtcttgcac tcgggcacca aatatatcaa cgggcacagc
    10981 gatgtggtca tgggcgtatt gtgtggtaag agggaaatga tcaaaaaaat attttacagc
    11041 gaattcatga atatcgggca tatactcagt ccccatgacg ctttcctggc tattcgcggc
    11101 ttgcgcaccc ttcccttgcg ggttcaacga tcgcacgaaa cttgcgcaaa attgatggag
    11161 gcacttcaaa accaccctaa agtggaaaag atccatcatc cgtttatcga tgagcagcag
    11221 gcgctggccg taagacaaat gaaaggcgga ggaatgttca cccttgccct taaagcggcg
    11281 cggaaagagc aggtgatcca attctgcgat tcgctggaaa attttctaat cgctgtatcc
    11341 tggggagggc atgaatcgct gatcatgcct gtggtaacct tttatgacat tcccggacag
    11401 ccggaccctg aacttccgtg gaacctggtc cggttttata tcggtctcga agatccgcac
    11461 tacctgctgg ccgatatcct gcaggccctg gaccggattt cctaaggaaa ggttaaccgg
    11521 ggcggtaagg aaacgactct agctttgatg ttctaatttt gcgcccgttt atgaaatcag
    11581 ggtgtctact gtttttgatt ttactttcct tccgggcctc cggcgcggac accctggacc
    11641 tcagcaggcc atttataatc caggcgggtc aatgcaggga gaaaatgacc atcgacggag
    11701 ccctggatga atccatttgg caaaatgcgc caaaagcctc cgccttccag gaacactggc
    11761 cgcaaaacgc aagggttcct ttgcataaga ccgaagccat gattgcatcc gatgatcatt
    11821 ccatttacgt ggcctttacc tgttatgata ccagcaagaa ttttatcatt caaaccctga
    11881 agcgggattt ccagttggat ggcaacgatg ctgtcgcggt gatcctggat ccgaccggac
    11941 aaaaaaactt tgcctacgcc ttccttcttt ccccggccgg tgtccaggga gaagccctga
    12001 tcaaccagtt cgaagcggat gccaattggg acagcaaatg gttttcggcg gtgaagatct
    12061 acgaggaccg ttggaccgcg gaattggcca tccccctcca catcctgcgt tatgatccca
    12121 accttttggt ctggaatgtt aatttcctga ggggagacgt aaaaaatttc cgcttttatt
    12181 cctggacaaa aatccctcaa cagtttgaca tttttaacct gggatattta gggtccctgg
    12241 tatggcctga accattgccc aaaaaaggag ggcagatcac cctgatcccc tacctgaatg
    12301 ccaacacctc cagggagttt gaagagcata cggtgcagaa gctcaacttt ggatttggag
    12361 gggacgctaa aatcaccctt tcctccagcc tgaacctgga cctcacggcc aatccggatt
    12421 tttcgcaggt ggaagtcgac cggcaagtaa ccaacctgac gagatttaat gtcttttttc
    12481 ccgaaaggcg taattttttc ctggaaaacg cggacgtctt cagccagttc ggcgatcccg
    12541 gaaatcccat ttttttttcc aggcggatcg gtctcgataa cgatggcaat gccgtaccca
    12601 ttctcggggg tgtccgtctg agcggcaata tgggcgaaac ttggaggatt ggggctttgt
    12661 ccatgcaaac caagggcacg gcggagcagg ccggtcagaa ttattcggcc ctgaccctgc
    12721 ataagaaggt gctcaaaagg tccctcatca agggcctggt caccaaccgg caggcggtgg
    12781 atggcattgt ggtgctgccc aaggattatg gacggaatgc ttctctggaa atgatttacc
    12841 agaatgaaaa gggttccttg ttcaactggc tgggcctcca taaatctttt aaacatgatc
    12901 tgaaggacaa aaacggccag ctcagcgcgg gcgtctggta caacgacaag aacttcagta
    12961 cagtcaccgg catcgggtcc acaggcacga attttaccgc cgacatgggt tttgtcaacc
    13021 agatcgacaa ctacgactca gaaagggata ccgtgatccg ccagggtttc aattccgcct
    13081 tcagtttcaa ccaatatata ctgcgcaccc cggaccataa attcatcaat cagcatcagt
    13141 tcagtctcaa caactcgttc aaatttcttc ccggggaaac cagcctgctg gattggagta
    13201 caagcctgga atacgaactc agttttaaat cctccagcga gattgaatgg tctgtccgca
    13261 ggaatcagct tcaattactt tatcctttct cttttacgga cggcagcccc ctgccccccg
    13321 ggaaatacag ttatgccaac gtgaatgcgg aataccgttc cgatctccgc aaacggtttg
    13381 gatttaccat gagcgcttcc gccggcagct tttacaacgg ttcccttacg gcgttcacag
    13441 ccggtatgct ttacagggcc cagccctggg gtaattttga ggtgaatttt gaattcaatg
    13501 accttgattt tccggaccct tttggccggg atcaattgat cctgatcaat ccccgcatcg
    13561 aattcaattt tactaaaaac ctgttttgga ccactttcct gcaatacaat acgcaggccg
    13621 acaactttaa catcaacagc cggttccagt ggagatacaa gccgatgtcg gatatttata
    13681 tggtttatac cgacaactat gccgtcaaat tctgggggcc caaaaaccgg agttttgtcc
    13741 tgaaaatcaa ttattggttg aacatttgaa caggccaggg cacccttcct tctgcagatc
    13801 ccttatcttt atttaatgct aaaaggcctg gtcctcttat ttcttttcat cgctcaaccg
    13861 atccgggcac aaaccgcttt tattcaggat gagaaattcg gaagggtctg gatcaagcct
    13921 gaacatccgg ccagttttac ccggacagat caggagcgca acctgcagga atattttaca
    13981 aaattttact ccgggcacgg ggacttggat tccggtttca aggcgctggt cctgctcacc
    14041 ctgatcatag acggagaagg ctttgccaac ctgtccaaag caaccgcttc caatccggcg
    14101 gtcagccttg aaacactgca aatcgcaaaa agcatccatg aaatgccgcg atggttcccg
    14161 gctgaggaga gggacaaaaa agtgcctttc caggttctct tgtccctgca atttgaaggg
    14221 aacaaagtat tgacgcgcta tttgaaattt taaccggctc acatggcgat cttaaggatc
    14281 tgcatgacga gaacgatgaa taaaatcccg taaaaagccc acatcgcccg tttaagattc
    14341 agaaattttt gtttgaggat atcgctgagt atggcggcct ggttgagcag gtcttccgtg
    14401 atctgatcgg taggcaattc caaaaaccgg ccgacaaagt ccttatcctg ctggctggca
    14461 atgtccatga aatagataag ttttttggtc tgagtatgat ggtcgttgtg ggaccggggc
    14521 atcatggtct tcatggaaaa atagatcaac aggccggata ccaagagaag catggcgaag
    14581 caggctttaa agaccgggtt gagggtggcc agctccttgt attcgctgaa aatgatggac
    14641 agcacgatcc cggcgatcat atacagcagg tatactttat aatccgcaaa ttggatcatt
    14701 tcctggaggg aggtaaacag ccgccaggcg tgatcggcct gcaattcttt cttcggccgg
    14761 tgggtgatga tttcgttcat atctcagagc cagtaaaagg caggcaaatg taggaaagcc
    14821 gccggaatcc cccaatccgt ctttccaggg tccccgaagt gagcccgaat ctccctgctt
    14881 cttactaatt ttaacccgtg gtctcctggt ctgtcaccga tttcctcacg gtaagtttta
    14941 ccctgtttgc cgtgatcgat gtcatcggtt ccgtgccatt attgatctcc ctgaagtcaa
    15001 aaatgggcgg catcgaggaa ttccgggcca cctttctttc cggagggctc atgatccttt
    15061 ttttatttgt cggcgaaaaa ctgctcaacc tcatgggggt cgatctgcgg tcctttgcgg
    15121 tggccggttc catcgtgatc tttatccttg gccttgagct tgtcctgggc attgaatttt
    15181 ttaaaaccga tgcccaggtc caatccggta ccctggtccc catagccttc ccattaatag
    15241 caggttccgg aacgctgact acggtgattt ccctaaaagc caattactcc accacggtca
    15301 tcctgaccgc catcctggtc aacctggcca ttgtttacct ggtcttaaaa tccctcaatc
    15361 tgattgaaag agccctgggt cccggtggtt tgcttgcggt gagaaaattt ttcggcgtga
    15421 tcctccttgc cattgccgta aaaatctttg cggccaacct tcccgggctt cttccgggat
    15481 aaaaagccat tgaatccggg cactaga
//
LOCUS       DAOQXT010000106        15154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4395_length_15154_cov_5.565932, whole genome shotgun sequence.
ACCESSION   DAOQXT010000106 DAOQXT010000000
VERSION     DAOQXT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15154
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4395_length_15154_cov_5.565932"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..893)
                     /locus_tag="PKM27_15655"
     CDS             complement(372..893)
                     /locus_tag="PKM27_15655"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR08755.1"
                     /translation="MKHNLLFVSLLACTMGFAQIPDQAVQKETIAFLGFNEQAITSLA
                     KAIPEDKYGWKPADGVRSVREVLLHVAGGNYFLLMNMGIAPPPTIDLMGMEKMEADKD
                     KILEILQASFKHVSDNLLSFKDSQLSEKVKFPWAEMTRHAACFLLIDHTGEHKGQLIA
                     YARSNGIVPPWSN"
     gene            complement(982..3678)
                     /locus_tag="PKM27_15660"
     CDS             complement(982..3678)
                     /locus_tag="PKM27_15660"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNR08756.1"
                     /translation="MKRRCFFVLGLLFISLQGIRSQTISGTVTDDKGLAMPFATIQVF
                     KTDKGGVSNQDGYYSIETGPGVFQLRYQFVGYRNLDTLVTVTNRDLTIHVRLRPEALT
                     LPTAIIDGSNEDPAYTIMRRTIAKAPYHANQVETYGAMVYIKGSGRLLKTPFLFRNRI
                     KKELAKEGIDSTVAFTQESVSRLSYKRPGQYRDTVISVRSTGDANNTSPMSFVYSSFY
                     DPKVAGAVSPLAPNAFQIYTFEYLGYIEDHGKVINKIKVTPRGRGDQVFEGMLYITDQ
                     LWSIHSLDLVTSLWGIRFAIKQVFAPASGDVWMPIDQIYDVTGDVFGFGFAYKYLAHL
                     SNYTITLNPDIQVPLVVLDDKIDKLEAKAANEKINKNPSPLSFSGLEPGQELSAKQLK
                     KMMKEYKKQELESLPEADTVAISFNESKQTVDSQAYKRDSVYWAEIRPVALTLHEIRG
                     YSRMDSLSEVEKQKAAEKQEDSSTLSITMGPDGTRMNVTKTHSAFKLQHLLFGSRYNL
                     NKTHYISILPTIGTFNFNTVDGYHAGFGLETGNIKKKNKRFEWKLRPEIRYAFSREAV
                     NWKLSGLLQKKSGKTGDGPWALKLEGGNAVDSYNPFLPENSYLNGLYTLLFRKNYLKL
                     YEKKYLGLAYEKTFSRSFSLKASAEWADRTILENHTNYSFFNRKRIYLSNQPNTFENS
                     APLFEAHQALVTDLSVRLVPFQRYVAQGAVKVLDDSKSPVITLGYKRGWEAGDSRFDH
                     FYLGWKHHIGIGAGDDYDYAIQAGSFVGNTTPYFQDFAQFAGNRYVFSPSDPVKYFRL
                     LDYHLYSTNDSYLSLLSNYQFRRFIFTTSPWVRKKGIRENVMINVLKTPGTELYTEFG
                     YSINYILRVLRLEVVTGWQDMKYKETGFRFGVATGFKNLFKF"
     gene            3841..6054
                     /locus_tag="PKM27_15665"
     CDS             3841..6054
                     /locus_tag="PKM27_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNR08757.1"
                     /translation="MQFRSGLFFLISIFFLNTRAGGQAFKALDIPVVIGTKTLLYPWA
                     GGLNAPQIHNADLNRDGSTDVVIFDRVGGAWLPFTWEGKLVYRPDLKSLFPRLKEWVV
                     FKDHNADGIQDIFSYSTTPGIAGIDVYDGQGTPGSLQWVKRSFPNDRANILFYTSGSS
                     RLNVYVSNIDYPAIEDIDRDGDLDVLSYETGGSLVIWYKNLAADKKLAAGSFDFVIGE
                     FCYGKFVEDGFSEAITLSNNPNKCASGLQPELEVRHAGSTTMAFDRDGDSDYDLLIGD
                     ISSSGLIYLRNGGDTLSAYMDAQETHFPVHSKPVDIPYFVSPFLADVNNDGLKEFFAA
                     SNFQFGSDNYHCLWKYEVDPANKQNFLYNSNAYLVDEMIDLGENTFPALVDVNGDGLL
                     DLVVGSGGYFDRNGLTRAGLALFTNTGTATKPSFQLTDSNWLDFRKYGPESYSFAPAF
                     GDLDGDGDLDLLVGEVLGRLYFAVNTGGKDKAMVFNSVLTGYSGIDVGQYSAPAVEDL
                     DGDGLPDLVVGERNGNVNFFKNTGSAQAAQFSAIPTLDNLGSIDTRVPGFATGNSAPF
                     FFRSNQKRYLAVGTSAQDILLYESPSAVSTPFNLVSTRWGAIHEGDETHPALADIDRD
                     GKLDILTGNLRGGLAWYQSDLPSEFSTAYRSQGEGFSISIYPNPARLTTVLTTGDRQL
                     RGKLDILDLSGRLIKAYPFTGNRLELDVSVYPPGVYLARISSTSGQYSKKLVIAR"
     gene            complement(6058..6498)
                     /locus_tag="PKM27_15670"
     CDS             complement(6058..6498)
                     /locus_tag="PKM27_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08758.1"
                     /translation="MLREEAISKVLEEAYRHEKMNLYDACFNLFSESETHLSDCKAYA
                     DELIDEKLAKYIDEKKTMLEITNYGKFWMLKGGYIEYLKQSEKKIKNEKVSLEDRLEE
                     ELVEARLKFTRYRIKTFWISFLLSILGFLFSLISLLLVFFKLKG"
     gene            complement(6505..7335)
                     /locus_tag="PKM27_15675"
     CDS             complement(6505..7335)
                     /locus_tag="PKM27_15675"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNR08759.1"
                     /translation="MGSPFHDPEREGREISLAFSPCPNDTFMMDALVNRKLPSGPDLK
                     VHLMDIQQLNQAAVAATFDVTKISVAAYPLVSDQYELLDAGAAMGFGCGPLVVAKTAL
                     KEKDLDRLKIAIPGRHTTANYLFHHFFPSAGQPFFTVFSEIENLVLSGEAGAGVIIHE
                     SRFTYRQKGLFCVADLGALWEERYHLPLPLGIFVCRRSLDPGLKENMNHLIRDSIRYA
                     FDHPASPSAYVLNHAREMEPEVVKQHIGLYVNEYSLSMGTAGRAAIDFLIQSENLIHK
                     "
     gene            7318..8025
                     /locus_tag="PKM27_15680"
     CDS             7318..8025
                     /locus_tag="PKM27_15680"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNR08760.1"
                     /translation="MEWTPHILYEDNHYIAISKPSGIPVQAESTDEDGVIPRLQEFIG
                     KRDQKPGKAFIGVLHRLDKAVTGVLILAKTSKGQERFNELLRKKEVRKFYFAVTEKNK
                     MPVEGTLTHFFSRKGEEYKMSVSLRKGSEKDQEAILKYSILAVQEDHQLLDIELITGR
                     RHQIRAQLAYMGCPILGDKRYGSKFQAPGIALHCCRIQFIHPVKKVPLNISCLPYGMD
                     EVWKPFRKKMVAVVNSQ"
     gene            8091..8188
                     /gene="ffs"
                     /locus_tag="PKM27_15685"
     ncRNA           8091..8188
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKM27_15685"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8242..8898
                     /gene="fsa"
                     /locus_tag="PKM27_15690"
     CDS             8242..8898
                     /gene="fsa"
                     /locus_tag="PKM27_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNR08761.1"
                     /translation="MKFFIDTANLDQIREARDLGILDGVTTNPSLMAKEGITGKENII
                     KHYQAICELVKGDVSAEVIATDFEGIVREGEELAALAPNIVVKVPMIKEGIKAIKYFS
                     DHKIATNCTLVFSAGQAILAAKAGATYLSPFIGRIDDTNWDGMQLIAEIAEIFSLQGF
                     ETQILAASIRNSMHIVSAAKAGADVVTCPLPAILGLLKHPLTDIGLAQFLADHKKANS
                     "
     gene            complement(8904..9776)
                     /locus_tag="PKM27_15695"
     CDS             complement(8904..9776)
                     /locus_tag="PKM27_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNR08762.1"
                     /translation="MAIAPSELILNSKGHVYHLDLHPDMITPVCITVGDPDRVSMVSR
                     YFDQIEVIRTHREFITHIGRLGTRNLMVISTGIGTDNIDIVLNEIDALLNIDLETYLP
                     KKHTTSIRFIRIGTSGSFHHGIPVDSRVVNPLAFGIDSLMKFYPGNKISPDAGWLSGL
                     EPDAKELFTDAYLGWANEDLEKWVGAGMHRGIAISCPGFYGPQGRNLRLQSNISVQLL
                     DQLATLSYENFPFTNFEMETAGLYSLSKALGHEAVSVNTILANRVNGQFSKDPEKSVD
                     GLIREVLEKIDKQE"
     gene            9945..10340
                     /locus_tag="PKM27_15700"
     CDS             9945..10340
                     /locus_tag="PKM27_15700"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNR08763.1"
                     /translation="MKKIFHLSTCQTNQKILKDWKPGKEFTLQNIKEKNISAEELDIA
                     AGVLGSYEALFSRRALKYKTMGLADKKLSEKDYRKLILSEYTFLKRPVLINGKEVFAG
                     SAKESVEGGRRSVRGREQKGERRRVKGEG"
     gene            complement(10548..12197)
                     /locus_tag="PKM27_15705"
     CDS             complement(10548..12197)
                     /locus_tag="PKM27_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HNR08764.1"
                     /translation="MKRYLKIHDQDNVVVALEEFKKLDKLEFNGQSILISENIPVKHK
                     IAIRDFLRGEAVKMYGLTVGRAKTPILKGSRISTENTEHATESFDTLQVKTDWKKPDI
                     GRFQGRTFKGYHRKDGSVGIRNYWIILPLVFCEKRNVAVIQDSLVKALGYQTVEEFGV
                     DTRLLVDLFRSGAAAPELLKADILRTQAEIHNRRVFKNIDGIKILNHDMGCGGTRQDA
                     DSLCRLLAGYVLNPNVAGATVLSLGCQNAQVSLFQEALDRFNPGHGKPVYIFEQQKYS
                     SERELIADAVKQTFCGLIEADKIERTPAGLEHLSLGLECGGSDGFSGITANPALGHAS
                     DLIVGLGGTTILSEFPELCGAEQDLINRCETPENAEKFARLMKTYSARAEAVGSGFYA
                     NPSPGNIRDGLITDAMKSLGAALKGGTAPVKGVLDYTERVSEKGFYLLCTPGGDVEST
                     TAMTSSGCTMIAFTTGLGTPTGNPVAPTIKIATNTALARRMPDIIDLNAGTIVDGADT
                     IPSKGEEILEYVIRVASGEAIPHAERLAQDDFIPWKRGISL"
     gene            12263..12961
                     /locus_tag="PKM27_15710"
     CDS             12263..12961
                     /locus_tag="PKM27_15710"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNR08765.1"
                     /translation="MNQELVIKSSTKQAVIFGASGLIGGFCLEYLLNHPAYSSVLVFG
                     RRVPGFRHEKMTAFQVDFENMEAWAGQVRGDDLFICLGTTMKKAGSKKAFYHVEYDYI
                     TDLVAYAELHRMKQVMLVSAMGADSSSVFFYNQVKGKIEESVRCRDFWSIHIFRPSVL
                     IGSREELRPLESFAAGAGMALRVLGPKLFAGITPIEGEKVAMSMVRAAQGTRSGVFYY
                     PSLVMAKMGKKEQN"
     gene            12988..13329
                     /locus_tag="PKM27_15715"
     CDS             12988..13329
                     /locus_tag="PKM27_15715"
                     /inference="COORDINATES: protein motif:HMM:NF040082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HNR08766.1"
                     /translation="MDQRKNKIVLEISLDEQDIPSSIHWTADEGPEAGNSQEARAFLL
                     SLYDAPSGDTLKIDLWTKDMQVHEMNRMMYFTLRSLADTYLRATANKELSTDLRRFTD
                     YFAEKCQLKNN"
     gene            complement(13351..13860)
                     /locus_tag="PKM27_15720"
     CDS             complement(13351..13860)
                     /locus_tag="PKM27_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR08767.1"
                     /translation="MIIGIGGVSRAGKTTLSEKLKKRLKGNSIKLFNQDDYVKKTGLP
                     IIKNHIDWEHPDSIDWKRLKKEVRAARATTAFVIVEGLFAFYDRDLARTYDHSVFVQI
                     DREVFFSRKSRDLRWGKEPAWFIRHIWNSYQKYGKLPRTIKEVIWVNGAMHTDLDPLV
                     RLILAGAGS"
     gene            13884..14978
                     /locus_tag="PKM27_15725"
     CDS             13884..14978
                     /locus_tag="PKM27_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08768.1"
                     /translation="MTNFHFKGGFILAFSLLITCSPPAVITRTDGTDPLRYEGEKHFK
                     NITQLTFGGNNAEAYWSFDSKHLTFQSDFGSWSNGCDQIFLMTPGKDDLKNNKPVRIS
                     TGTGRTTCSYFMPGNKKILYASTHAAGSTCPPAPERTGGKYVWAVYESFDIYVADLEG
                     KIVSRLTDHPGYDAEATVSPKGDKIVFTSVRDGDLDLYTMDTDGKNVKRVTSELGYDG
                     GAFFSPDGSKLVFRASRPQTEEAKKEYLDLLKQGLVQPTEMEIFVCNTDGSGLKQVTK
                     LGKANWAPYYLPDNKRILFSSNHAASRGYSFNLYMINEDGTGLEKVSFDQTFDSFPMF
                     SPNGKYLVFASNRFNGGTRDTNLFVTEWVE"
BASE COUNT         3698 a         3690 c         4008 g         3758 t
ORIGIN      
        1 gatggcggct ataccgagaa tggtcaaatc aaatacgccc aggttccgtt taagatgagc
       61 aaacaggccc tcctcctccg tcagcgccgc atcctttaat attttttcaa ccgatttttt
      121 acgaaacaaa cctccactca tagatcattg gataatatgt ggaatgaaga taaagcaaat
      181 tgccaaaccg gtgaatgttt gcacgaactg gcagggatta ggacaagcgg tgtctttgga
      241 atgctacgga agttggaaca gcagtgttat tgaatgggca agccgataaa ctgatcaacc
      301 ggcaaactga aaaactgact actgattact gactactgac tattgactac tgactactga
      361 ttcctgacca ctcaattact ccagggcggg acaatcccat tggaccttgc atatgcgatg
      421 agctggcctt tgtgctcgcc tgtgtgatca atcagaagga aacaagctgc atgtctcgtc
      481 atttcagccc atggaaactt gactttttcg ctaagctgtg aatctttaaa actaagtagg
      541 ttgtcgctga catgcttgaa cgatgcctgc agtatttcca ggatcttatc cttgtccgcc
      601 tccattttct ccattcccat gagatcaatc gtcggagggg gtgctattcc catattcatc
      661 aggagaaaat agttacctcc ggctacatgc aaaaggactt cgcgcaccga gcgaactcca
      721 tcagcaggct tccatccgta tttatcttct ggaatcgctt ttgccaggct ggttatagcc
      781 tgttcgttaa agccgaggaa agcgatcgtt tctttttgaa cggcctgatc cggaatttgg
      841 gcaaaaccca tggtacaggc cagtaatgaa acgaaaagta gattatgctt catgattttg
      901 ttttttggtt ggtttgaaat taatcatttt ttggcgaccg gaagtgatta atctatttgt
      961 tgttccaatt cagaacccgc ctcagaattt aaacaggttc ttaaacccgg tcgccacccc
     1021 gaaacggaaa cccgtttcct tgtacttcat atcctgccaa ccggtgacga cttccaggcg
     1081 gagaacccgg agtatatagt tgatgctgta cccaaattct gtatacagtt ccgtccctgg
     1141 cgtcttcagt acattgatca tcacgttttc ccggatgcct tttttcctta cccagggtga
     1201 agtggtgaaa ataaaacggc ggaactggta attggaaagc aaagaaagat aagagtcatt
     1261 ggtgctgtac agatgataat ccagcaagcg gaaatatttt acgggatccg atggggaaaa
     1321 cacatagcgg tttcctgcga actgggcgaa gtcctggaaa tagggcgtgg tgtttcccac
     1381 aaaagatccg gcctggatgg cgtaatcgta atcgtccccc gcgccgatgc caatatggtg
     1441 tttccatccc aggtaaaagt gatcaaagcg gctgtccccc gcctcccagc ctcgcttata
     1501 accgagggtg atgacgggcg atttggaatc gtcaaggact tttaccgcgc cctgggccac
     1561 gtatcgctga aaggggacca ggcggacgga caggtcggtc accagggcct ggtgggcttc
     1621 aaataaagga gcggaattct caaaggtgtt gggctggttg cttaaataga tccgtttcct
     1681 gttgaaaaag ctgtagttgg tatggttttc caggatggtg cggtccgccc attctgccga
     1741 agctttcagg gaaaaggagc gtgaaaacgt tttttcgtaa gccaggccca ggtatttttt
     1801 ctcatagagt ttgaggtagt ttttacggaa gagcagggtg tagaggccgt ttaaatagga
     1861 attttccggc agaaaaggat tatacgaatc cactgcattg ccgccttcca gcttcagggc
     1921 ccagggtccg tcgccggttt tacctgattt tttctgcagg agtccggata gtttccagtt
     1981 tacggcctcc ctggagaagg catagcggat ttctggcctc agtttccatt cgaagcgttt
     2041 attcttcttt ttgatattac cggtttccag tccgaatccg gcatggtaac cgtcgaccgt
     2101 attaaaattg aaggtcccga tcgtcggtaa aatggagata taatgggttt tgtttaaatt
     2161 ataccgggag ccaaacaaaa gatgctgcag cttgaaagcg gaatgggttt tggtgacgtt
     2221 catccgggtg ccatccggac ccatagtgat gctgagcgtc gaggagtctt cctgcttttc
     2281 cgccgctttt tgtttttcga cttcggagag gctgtccatc cggctataac cccggatttc
     2341 gtggagggtc agggccaccg gtcggatctc cgcccaatac acactatctc ttttataggc
     2401 ctggctgtcc accgtctgct tgctttcatt aaaggatata gccacggtat ccgcctcggg
     2461 taagctttcc agttcctgtt ttttatattc cttcatcatt tttttcagtt gtttggcgct
     2521 gagctcctgt ccgggttcca gcccggaaaa actgagaggg gaaggatttt tattgatctt
     2581 ctcattggcg gctttggctt caagcttatc gatcttatca tccagcacca ccaggggcac
     2641 ctggatgtcg gggttgaggg ttattgtata attggacagg tgcgccaggt atttataggc
     2701 aaaaccaaaa ccaaaaacat ctcccgtgac atcgtagatc tggtcgatcg gcatccagac
     2761 gtccccgctg gcgggggcaa atacctgttt gatggcaaag cgtatgcccc agagagaggt
     2821 caccaggtcc aggctgtgaa tgctccaaag ctgatcggtg atgtagagca ttccttcaaa
     2881 cacctggtcc ccccggcccc tgggggtcac cttgattttg ttgatcacct tgccgtggtc
     2941 ttcgatatat cccaggtatt cgaaagtata gatctggaat gcgttggggg caagcggaga
     3001 gaccgccccg gcaaccttgg gatcataaaa gctggaatac acaaaactca ttggactggt
     3061 gttgttggca tccccggtcg aacgtacgga tatgacggtg tcccggtatt gtccgggcct
     3121 tttataggat aggcgggaaa cggattcctg ggtgaaggca accgtggaat caatgccttc
     3181 cttagccagt tcttttttga tccggttgcg aaagagaaag ggcgttttga gcaaacggcc
     3241 gctccctttg atataaacca tagccccgta ggtctccacc tggttggcat gatagggggc
     3301 tttggcgatg gtcctccgca tgatggtgta ggcagggtcc tcattgcttc cgtcaatgat
     3361 ggcggtgggc agggtgaggg cttcggggcg gaggcgcaca tggatcgtca ggtccctgtt
     3421 ggtcacggtc accagcgtat ccaggttccg gtaacctaca aactgatacc tcaactggaa
     3481 tacgcccgga ccggtctcga tgctgtaata accatcctgg ttggacacgc cgcccttatc
     3541 cgttttaaac acctggatgg tggcaaaagg catggcaagc cccttgtcgt ccgtcaccgt
     3601 acccgaaatc gtctggctcc ggatcccctg gagactaata aacaataatc ccaggacaaa
     3661 aaaacatcgc cttttcatgc ctcaaaatta ggggaatcgc ctttaagacc cgggggcttt
     3721 cgaccaggcc ggcctgacga cctctcaaag cggtggaata gccgatttat aatattgggg
     3781 agttatggac aaatggattt gtccggtacc atgccgtatt tatttcaagt aatttcgcgt
     3841 atgcagtttc gttccggttt attcttcctg atcagcatat tttttttaaa tacccgggca
     3901 ggaggacagg ccttcaaagc cctcgatatt ccggttgtga tagggactaa aaccctcctt
     3961 tatccctggg cgggaggact gaatgcaccg cagatccaca atgcggacct caaccgggac
     4021 ggatcgacgg atgtagtgat ctttgaccgg gtagggggcg cctggctgcc ctttacctgg
     4081 gaaggcaaac tggtttaccg gcccgacctg aaatccctgt ttccaaggct caaggagtgg
     4141 gtggttttta aagatcacaa tgccgatggg atccaggata ttttttctta ttcgactaca
     4201 ccgggcatcg cgggcatcga tgtgtacgat ggccagggaa ccccaggttc gcttcaatgg
     4261 gtcaaaagat ccttccccaa tgaccgggcg aatatccttt tttatacctc cggatcgagc
     4321 cgcctcaacg tctatgtcag caatatcgat tatccggcca ttgaagacat agaccgggac
     4381 ggggacctgg atgtactgag ctatgaaacc ggcggcagcc tggtcatctg gtataaaaac
     4441 cttgccgcgg ataaaaagct ggcagccggc agttttgatt ttgtgatcgg ggagttctgt
     4501 tacgggaaat tcgtggaaga cggtttttcg gaagcgatca ccctgagcaa caatccgaac
     4561 aaatgcgctt cgggccttca acctgagctg gaagtacggc acgcaggctc taccaccatg
     4621 gcttttgacc gcgacgggga cagtgactac gatttgctta tcggggatat ctcttcctcc
     4681 ggcctgatct atctccgcaa tggaggggat accctctccg cctatatgga tgcccaggag
     4741 acccattttc ccgtccattc caaaccggtg gatattcctt atttcgtaag ccccttcctg
     4801 gccgatgtaa acaatgacgg actgaaggaa ttttttgcgg cttccaattt tcagtttgga
     4861 tcagacaatt accattgttt gtggaaatat gaggtcgatc cggcaaacaa acagaatttt
     4921 ctttataaca gcaatgccta cctcgtggat gaaatgatcg acctgggtga aaacaccttt
     4981 cccgccctgg ttgacgtcaa cggggatggg cttctggacc tcgtcgtcgg ttcgggcggc
     5041 tattttgacc gcaatgggct tacccgcgca ggactggcgc tttttaccaa tacgggaacg
     5101 gccaccaaac cttcctttca attgaccgac agcaactggc tcgacttcag aaagtatggt
     5161 ccggaatcgt acagttttgc cccggccttc ggagacctgg atggagacgg tgacctggat
     5221 ctcctcgtcg gagaggtatt gggacgacta tattttgcgg tcaatacggg aggaaaggac
     5281 aaagccatgg tttttaattc cgttcttacc gggtacagcg gaatcgatgt gggccagtat
     5341 tcggcgcctg ccgttgaaga cctggatgga gacggccttc ctgaccttgt agtcggggaa
     5401 agaaacggga acgtcaattt ttttaaaaat acaggcagcg cccaggccgc gcaattttct
     5461 gcaattccaa cgctggacaa tttgggttcc attgatacgc gtgtaccggg ttttgccaca
     5521 ggcaattcgg cccccttttt tttccggagc aaccaaaagc gttatctggc ggtggggacg
     5581 agcgcccagg atatcttatt gtatgaatcg ccctccgccg taagcactcc ctttaacctg
     5641 gtatccactc gctggggcgc cattcacgaa ggggatgaaa cccatcccgc ccttgcggat
     5701 attgaccggg atggaaaact ggatatcctc accgggaatc tgaggggagg cctggcctgg
     5761 taccagagcg atctgcccag tgagttcagt acggcctacc gatctcaggg ggagggattt
     5821 tcaataagta tttatcccaa tccggcccgg ctgactaccg ttttgacgac gggagaccgc
     5881 caactaaggg gaaaattgga tatcctggat ctttccggta ggttgattaa ggcctatcct
     5941 tttaccggta accgccttga actggatgtg tcggtttatc cgcccggggt ttacctggcc
     6001 cggatttcat ccacttccgg acagtatagc aaaaaactgg tgatcgccag gtaaaattca
     6061 gcctttcagt ttgaagaaaa caagcagcag gctgatcagg gaaaaaagaa aacccaggat
     6121 ggagagcagg aatgaaatcc agaatgtttt aatccggtag cgggtgaatt tcaggcgcgc
     6181 ctctacaagc tcctcttcca gccggtcctc cagggaaacc ttttcattct tgatcttttt
     6241 ttcactttgt ttcaggtatt caatataacc gcccttgagc atccagaatt tgccatagtt
     6301 ggtgatttcc agcatggttt ttttttcatc gatgtatttg gccagttttt catcgatgag
     6361 ttcatcggca taggccttgc aatcgctgag atgggtttcg gattctgaaa acaggttgaa
     6421 acaggcatcg tacaaattca ttttctcatg cctgtaggcc tcttccagga cttttgagat
     6481 ggcttcttcg cgtaacacgg taatctattt atggataagg ttttcggatt gaatcaggaa
     6541 atcgatggcc gccctgccgg cggtgcccat ggaaagactg tattcgttga cgtaaaggcc
     6601 gatgtgttgt tttaccacct ccggttccat ttcacgggcg tggttcaaaa cataagcgct
     6661 gggggatgcc gggtgatcaa aggcgtagcg gatgctatcc cggattaaat gattcatgtt
     6721 ttcttttaat ccgggatcca gggacctacg gcagacaaaa attccgaggg gcagaggcaa
     6781 gtggtatctt tcttcccaaa gcgcacccag gtcggcgacg caaaacaaac ccttttgccg
     6841 gtaggtgaaa cggctttcat gaatgatcac ccccgccccc gcttcgcccg acaataccag
     6901 gttttcaatt tcagagaata cggtgaaaaa gggttggccg gcggagggga aaaaatgatg
     6961 gaacaaataa tttgcggtgg tatgcctgcc cggaatagcg attttcagac ggtcaaggtc
     7021 tttctccttc aaggctgttt tagccacgac caggggcccg catccaaaac ccatggccgc
     7081 gcccgcatcc aataattcat attgatcaga taccaaggga taggccgcca cactgatctt
     7141 ggtgacatcg aaggttgcgg caaccgcggc ttggttgagt tgctggatgt ccattaaatg
     7201 caccttcaaa tccgggccgg agggcaattt acggttgacc agggcatcca tcataaaggt
     7261 atcgttgggg cagggagaaa aggcaaggct gatttcccta ccttcacgtt ccggatcatg
     7321 gaatggactc cccatatttt atacgaagat aaccactata tcgcgatctc taagccgtcg
     7381 ggcatcccgg tacaggccga atcgacggat gaagacgggg tgatcccgag gctgcaggaa
     7441 tttatcggca aacgcgatca aaaaccggga aaagccttta tcggagtgct ccaccggctc
     7501 gacaaggcgg tcaccggcgt cctgatactg gccaaaacct ccaaagggca ggaacgtttt
     7561 aatgaactgc tcaggaagaa ggaagtcagg aagttttatt ttgcagtaac ggagaaaaat
     7621 aaaatgccgg tggaaggaac cctcacccat tttttctcca ggaaagggga ggagtataaa
     7681 atgagcgtga gcctccggaa gggtagcgaa aaggaccagg aagccattct caaatactcc
     7741 atcctggccg tccaggagga ccatcaatta ctggacatag aattgatcac cgggaggagg
     7801 caccagatcc gggcccaact agcctatatg ggatgcccca tcctcggtga caaaagatac
     7861 ggaagcaagt tccaggcgcc gggcatcgcc ttgcattgct gcaggatcca gtttatccac
     7921 ccggtcaaaa aggttccgct gaacatatcc tgtttgcctt atgggatgga tgaagtgtgg
     7981 aaacctttta ggaagaaaat ggttgcagta gtcaatagtc aatagtcagt tatgcaacaa
     8041 ttctacaatt caacaattcc gcaattcaac aattccatta cctttggccc cggtgagaat
     8101 tatgcaatca gctcctgttg aacctcccca gggtcgaaag gcagcaagga taaaacggtt
     8161 gagcgatgtg tatgatttct caccattttt ttttgacttt tgctgtgaat accaataaat
     8221 tatactcaat cgcaacctcc tatgaagttt tttattgata ccgccaacct ggaccagatc
     8281 cgggaagcca gggacctggg aatcctggat ggggtcacga ccaatccctc cctgatggcc
     8341 aaagagggga tcactggcaa agaaaatatc atcaaacatt accaggcgat ctgcgagctg
     8401 gtcaaaggag atgtcagcgc cgaagtcatc gctacggatt ttgaagggat cgtccgggaa
     8461 ggggaggaat tggcggccct ggccccgaat atcgtggtca aggttcccat gatcaaggaa
     8521 gggatcaaag ccataaaata tttttcagat cataaaatcg ccaccaattg caccctggtg
     8581 ttcagcgccg ggcaggctat cctcgccgcc aaagccggag cgacctacct gtccccgttt
     8641 attggccgga ttgacgatac caactgggac ggcatgcaat tgatcgccga gattgcggaa
     8701 atattttccc ttcagggctt tgaaacccag atcctggcgg cctccatccg aaattccatg
     8761 cacatcgtct cggcggcaaa ggcgggcgct gacgtcgtca cctgtccgct tccggctatc
     8821 ttgggattgc tgaaacatcc gctcaccgat atcgggctgg cccagtttct cgcggaccac
     8881 aagaaagcca acagctgaca ggattattcc tgtttgtcga ttttttcgag cacctctcga
     8941 ataaggccat ccactgattt ttccgggtct ttgctgaact ggccatttac ccggttggcc
     9001 aaaatggtat ttacactcac cgcttcatgg cccagggcct tcgataagga ataaagtccg
     9061 gccgtttcca tttcgaaatt ggtgaagggg aagttttcat aggagagggt ggccagttgg
     9121 tccagcaatt gcacagagat attggactgc agtctcaggt ttcggccctg agggccataa
     9181 aaaccggggc aactgatggc aatcccccgg tgcatccccg cgcccaccca tttttccagg
     9241 tcttcattgg cccagcccag gtaggcatca gtaaacaatt ctttggcatc cggttccagg
     9301 ccactgagcc atccggcgtc cggactgatt ttatttcccg gataaaattt catcagggag
     9361 tcaataccga aggccagcgg gttgacgacc cgggaatcca ccgggatgcc gtgatgaaag
     9421 gaacccgagg tgccgatacg gatgaaccgg atagaggtcg tgtgtttttt gggcagatag
     9481 gtctccaggt caatattgag cagggcatcg atctcattca gcacgatatc gatattgtcg
     9541 gtcccgatcc cggtggagat caccatgagg ttccgggtac ccagccttcc gatatgggtg
     9601 atgaattccc ggtgggtgcg gatgacctca atctgatcga aataccggct gaccatggac
     9661 accctgtccg gatcgcccac cgtaatgcag accggggtga tcatatcggg gtgcaggtcc
     9721 aggtggtaga catggccttt ggaattgaga atgagttcgg atggtgcgat ggccatggat
     9781 cagttgccgc gggatttctg ctctaattta aaaaatccgg caaatccgct caagctctac
     9841 caaaacggat agtctgggga tagggaatct tcattgggaa aactcccatt gattcttcct
     9901 cctcgatggc cgtgggatac ggtataccgg cctaacttta caccatgaaa aagatctttc
     9961 atctctcgac ctgccagacc aaccaaaaaa tattaaaaga ctggaaacca ggcaaggaat
    10021 ttaccctcca gaacatcaag gaaaaaaata tcagcgccga agaactggat atagcggccg
    10081 gcgtgctggg ttcctatgaa gcgcttttca gccggcgggc cttgaaatac aaaacgatgg
    10141 gcctggcgga taaaaaattg tcggaaaagg attaccgcaa actcatcctt tcagaataca
    10201 cttttcttaa aagaccggtc ctgatcaacg ggaaggaggt atttgcagga agcgccaaag
    10261 agtcggtgga aggggggagg aggagtgtga ggggaaggga gcaaaagggt gaaaggcgaa
    10321 gggtgaaggg tgaagggtga agggtgaaag gcgaagggag caaaagggta aaagggtaaa
    10381 gggcgaaggg tgaaaggtga agggagcaaa tgggtaaaag ggtaaagggg gaagggtgaa
    10441 aggcaaaagg gagcatgagg tccaaattct ctgttaacag ctggaagcta gaagctagaa
    10501 ggtagaagct agaagctaga agctggaaac tagaagctgg aagctagtca cagactgatt
    10561 ccccgtttcc aggggataaa atcgtcctgg gccagtcgtt cggcgtgagg tatggcttcg
    10621 ccgctggcaa cccggatgac gtattcgagg atttcttcgc ctttggatgg aatggtatcg
    10681 gcgccatcta caatcgtgcc tgcattgagg tcgatgatat caggcatcct gcgggctaag
    10741 gcggtgttgg tggcgatctt gatcgtgggg gctaccgggt ttccggtcgg agtgccgaga
    10801 ccggtggtga aggcaatcat agtgcatccg ctggaagtca tggccgtagt ggattccaca
    10861 tccccgcccg gggtgcacaa caggtaaaaa cctttttccg ataccctttc ggtataatcc
    10921 aggacccctt tcaccggggc ggtgcctcct ttgagcgcag cgcccaggga tttcatggca
    10981 tccgtgatca atccgtcgcg gatattgccg ggagagggat tggcgtaaaa accggaaccc
    11041 acggcttccg ccctggcgct gtaggttttc atcagccggg cgaatttttc tgcattttca
    11101 ggggtttcac aacggttgat caggtcttgt tccgcaccgc agagctccgg aaattcggag
    11161 agaatggtgg tacctcccag gccgacgatc aggtcggagg catgtcccag ggccggattt
    11221 gccgtgatgc ccgaaaagcc atccgaaccg ccgcattcca ggccaaggct gagatgttcc
    11281 agcccggccg gtgttctttc tattttatcc gcttcaatca atccgcaaaa cgtttgtttg
    11341 accgcatcgg cgatcagttc tcgctcggag gaatacttct gttgttcaaa aatatagacc
    11401 ggttttccgt gccccgggtt aaaacggtcg agggcttcct ggaaaaggga gacctgggcg
    11461 ttctggcagc ccaggctcaa cacggtcgcc ccggccacat tgggattgag tacatatccc
    11521 gcaagcagtc ggcaaaggga gtccgcatcc tggcgcgtcc ctccgcatcc catatcgtgg
    11581 ttaaggattt tgattccgtc gatattttta aatacgcggc gattgtgaat ttccgcttgg
    11641 gttctgagaa tatcggcttt aaggagttcc ggggccgctg caccggaccg gaataaatcc
    11701 acgaggagtc gggtatccac accgaattcc tcaacagtct ggtaaccgag cgctttgacc
    11761 agggagtcct ggatcacggc cacattccgc ttttcacaaa aaacgagggg gaggatgatc
    11821 cagtaattgc ggatccctac cgacccgtcc ttgcgatggt aacccttgaa agtccttccc
    11881 tggaagcggc cgatatccgg ttttttccaa tcggttttga cctggagggt gtcgaaggat
    11941 tcggtggcgt gttccgtatt ttccgtgctg atccgggagc ccttgaggat gggggtcttg
    12001 gccctgccca cggtaagacc gtacattttc accgcttctc cgcgcagaaa atcacggatg
    12061 gcaatcttgt gtttgacagg gatgttttcc gaaatgagga tagactggcc gttaaattcg
    12121 agtttatcca gctttttgaa ttcttccagt gcgaccacta cattatcctg atcgtggatt
    12181 ttcagatatc ttttcatgcc ttgtatttgg ggaggtgcaa tacgggtcaa aattaagctt
    12241 ttggtcattt atcttcgccg ggatgaatca ggaattggtg atcaaatcgt cgaccaaaca
    12301 ggccgtgata ttcggcgctt caggcttgat cggaggattt tgcctcgagt acctgctcaa
    12361 tcacccggcc tattcgtccg tcctggtctt cggccgccgg gttcccgggt tccggcatga
    12421 gaaaatgact gccttccagg tggattttga gaatatggaa gcctgggcag gccaggtccg
    12481 gggtgatgat ctttttatct gcctcggaac caccatgaag aaggcaggtt ccaaaaaagc
    12541 gttttaccat gtggaatacg attacatcac cgacctggtg gcttacgcag aattgcaccg
    12601 gatgaaacaa gtgatgctgg tctcggcgat gggggcggat tcctcttccg tcttttttta
    12661 caatcaggtc aagggtaaga tcgaagagtc cgtgcgttgc agggattttt ggagcatcca
    12721 tattttccgg ccctcggtcc tcatcggcag cagggaagaa ttgaggccat tggaatcctt
    12781 tgcggccggg gcgggcatgg cgctgcgggt actcggccct aaactttttg ccgggatcac
    12841 cccgatagaa ggggaaaaag tagccatgtc tatggtccgg gcggcccagg gcacccgcag
    12901 cggggtattt tattatccct ccctcgtcat ggccaaaatg ggtaaaaaag aacagaactg
    12961 acggatttta ctctgtttct ttgcgccatg gatcaaagaa aaaataaaat tgttctcgaa
    13021 atcagcctcg atgagcagga tatcccctca tccatacatt ggacggcaga cgagggcccc
    13081 gaagcgggga acagccagga agcgcgcgcc tttctcttgt ctctttatga cgcccccagc
    13141 ggagataccc ttaagatcga tttgtggacc aaggatatgc aggtacatga gatgaaccgg
    13201 atgatgtatt tcaccctgag gagcctggcg gacacttacc tgagggccac cgccaataaa
    13261 gaattgtcca cagacctgag gcggtttacc gattattttg cagaaaaatg ccagttgaaa
    13321 aacaattagg ccgcccggct ttgaaaccgg tcaggaaccg gctcctgcca ggatcaaccg
    13381 gaccagggga tcaaggtcgg tatgcatggc cccgtttacc cagatcacct ctttgatcgt
    13441 acggggcagt tttccgtatt tctggtagct gttccagatg tggcggataa accatgccgg
    13501 ttctttcccc caccggaggt cccggctttt ccgggaaaaa aagacctccc tgtcaatctg
    13561 gacaaagacc gaatgatcat aggtccttgc caggtcccgg tcataaaagg caaagaggcc
    13621 ttccacgatg acgaatgcgg tcgttgccct tgcggccctg acctcctttt tcagcctttt
    13681 ccagtctatg gaatccggat gttcccagtc gatatgattt tttatgatgg gcaggccggt
    13741 ttttttaaca taatcatcct gattgaaaag ctttatcgaa ttacctttga gccgtttttt
    13801 taacttttcg gacaaggtcg ttttgcctgc ccggctaacc ccgcctatgc cgataatcat
    13861 gggcccaaaa ttaattaaag cagatgacga actttcattt taaaggaggc tttatacttg
    13921 ccttcagttt gttgatcact tgttctcccc ctgcggtcat tacccggacg gatggaacag
    13981 atcccctccg gtatgaagga gagaaacact ttaaaaacat cacccaactg acctttggcg
    14041 gcaacaatgc ggaagcgtac tggagttttg acagtaaaca cctgaccttt cagagcgatt
    14101 ttggttcctg gtccaacgga tgcgaccaga tttttttaat gactcccggg aaggacgacc
    14161 ttaaaaacaa taaacccgtc cgcatcagta cgggcaccgg gaggaccact tgttcctatt
    14221 ttatgcccgg caataaaaaa atattgtatg cttccacgca tgcggcagga agcacctgtc
    14281 cacctgctcc tgaacggacc ggcggaaagt atgtctgggc cgtttatgaa agttttgaca
    14341 tttatgtagc cgacctggaa ggaaaaatcg tttcccggct taccgaccat ccgggttatg
    14401 atgccgaagc cacggtaagc ccgaaaggcg ataaaattgt atttacctcc gtccgggacg
    14461 gggacctcga tctgtacacc atggataccg atggtaaaaa cgtaaaaagg gtgacttcgg
    14521 aactcggtta cgacggcggc gccttctttt ccccagatgg ctcaaaactg gttttccggg
    14581 cttcgcgccc gcaaacggag gaggcaaaaa aggaatacct cgacctcctg aaacaaggat
    14641 tggtccagcc taccgagatg gaaatttttg tctgcaatac ggacggttca ggcctgaaac
    14701 aggtgaccaa actgggcaag gcaaactggg cgccctatta tttaccggac aacaaaagga
    14761 tcctgttcag ctccaaccat gcggccagca ggggatattc tttcaatttg tatatgatca
    14821 acgaagatgg caccggcctg gaaaaggtca gtttcgacca gaccttcgat tccttcccca
    14881 tgttttctcc taacgggaaa tatctcgttt ttgcttccaa ccgcttcaat gggggtaccc
    14941 gggataccaa tctttttgtg acggaatggg tggaataaat aggtgtcccc caccggtagg
    15001 aggttagagg tcagaggtca gaggttagag atcccgcggc tgtaggagtt aaaaggagaa
    15061 aggtgaaggg cgaagggtga agggacacta attgctgaat tgctgaattg ttttgggact
    15121 acatttttat tccaagaatc aatttattac acat
//
LOCUS       DAOQXT010000107        14729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4543_length_14729_cov_6.814570, whole genome shotgun sequence.
ACCESSION   DAOQXT010000107 DAOQXT010000000
VERSION     DAOQXT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4543_length_14729_cov_6.814570"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            695..1297
                     /locus_tag="PKM27_15730"
     CDS             695..1297
                     /locus_tag="PKM27_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNR08769.1"
                     /translation="MEIELKSLNPKFVGLFRQTFAFTEEEFALFLSFFQFKTICKKEY
                     YLRAGELCKAKAYLNKGCARNFVVDEKGHERILFFPFEDWWLGDFDSYYSGKPATNYV
                     QMLEDSELLVISKEHFQKAEQEIPKLKQWYSSKMLSSASAARNRIEEMKTLSAEELYL
                     KLLEKQPHIFQRIPLQYIASYLNIEPQSLSRMRKRLLKNH"
     gene            1340..1879
                     /locus_tag="PKM27_15735"
     CDS             1340..1879
                     /locus_tag="PKM27_15735"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR08770.1"
                     /translation="MFFTPGIFVSGNLSITKNKNMTTLTTTQSRVLKPKEGLKLQSGP
                     GRELIYKVTGEDTGGAFDYFIVEVAPHGGPPLHVHHKQEETIHVLKGKFKVRIGDEIL
                     YCNEGDFVYLPSKVPHAFLNLTDEQAEIIVVYTPGGGHKFYEELGPLMRNGSPDKKVV
                     AELFEKYEMTILGPPLKQD"
     gene            complement(2016..2522)
                     /locus_tag="PKM27_15740"
     CDS             complement(2016..2522)
                     /locus_tag="PKM27_15740"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08771.1"
                     /translation="MIDIKKAGMEDWQKIRNIAFETWPVTYGTLMPMEQVHYLLEKLF
                     SRHTIQSQMNENGHVYLLAESEGIQVGFASYEINYRGQPRMMIHKLYVSPALQGNGIG
                     TQVLDALTGIASQKHQTALRLKVYYLNRKAIRFYEKYGFVKTGIEKTDPGNRFIIEDE
                     VMIRELLS"
     gene            complement(2591..3319)
                     /locus_tag="PKM27_15745"
     CDS             complement(2591..3319)
                     /locus_tag="PKM27_15745"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNR08772.1"
                     /translation="MQALVMDFNGIALKRKARLAGLLYLFLVFSGLYGLIYLPSKIPQ
                     GGDAATTAKNILANEFLFRTGLVNGMISGLLFLFVVLALYRLFEPVDSKQARLMVILI
                     VLQIPIGFVLEVFPLTSLMILKGEALKFLEPGKQEQVALILLEIGKQGTLSLEFFWGL
                     WLFPFAWLVWKSGFIPRILGLFLMLNGLAYILEWFTLLLFPSYKAQMRVWAFPFFFGE
                     LAITLWLLLIGIRKKAETHENVSP"
     gene            complement(3320..4660)
                     /locus_tag="PKM27_15750"
     CDS             complement(3320..4660)
                     /locus_tag="PKM27_15750"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNR08773.1"
                     /translation="MIRLFQTLFITFFIQGYLVGQIDPSAGLWKTWFISSGRDYRLPA
                     PGSYKDEIAQVLSIQQKADETTRLQIHYWNAGPPCYRWQEMMTRLWTLDTGRYGALAN
                     MLLGTAIYDATVAAWDSKYTHHRPRPFEADRRINIRIPPPESPSFPCEHSVAAGVAVS
                     VFSAFYPAMADSVRKLARQLMDSRVAAGVAFPSDTRAGFELGMRIAGEEIERTSDFVC
                     KETWDGKIPEGPQYWRGRFALLPMAGKNKTVVLSGPDEFRPGPPPDYAKEMEELRHYK
                     QNFRSLSNAFQWANADWFAEELKKKIFEYNLHLNPPLAAYIHAAMGVGLYDGFVACWD
                     AKYTYWGIRPNQYDTTFSPAILVTPPFPGYPSGHAAITAVEAGLMSYFFPEEKERYWK
                     KAAEAAESRFQAGIHFRSDNEAARELGKKVAEKLVQKLIREDHPGPMDKFSGNK"
     gene            4959..6014
                     /locus_tag="PKM27_15755"
     CDS             4959..6014
                     /locus_tag="PKM27_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNR08774.1"
                     /translation="MKWPLDIKKIKFYLWVGLAYFGLWSIGNLVGYRDTLWVRTVNDL
                     WRVLYIIAINFSFFEYALPFILAKRKSVLYNILWGLLIVWIFLMLWSFGLYGWRAIGI
                     ALGIYTELTRFKTVDEGVSYHFGYSVGSILFFGLVRHVFQYIQLRQSAQQLRIEKQQA
                     ELNYLKSQTNPHFLFNTLNNIYSLARDKSDLAPESIMRLSKILRYMLYETGGEYIAIE
                     QELKIIGDYIELEKLRYDESLRVNFNYDIEDMKQALPPLLLIPLVENAFKHGVSETRS
                     QPFVDIHLSVKERRLNFGVKNSVDPFSGGKALKENIGLANLRRQLELLYTDYRLAVNQ
                     GDSMFTAILYINLASHV"
     gene            6007..6699
                     /locus_tag="PKM27_15760"
     CDS             6007..6699
                     /locus_tag="PKM27_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR08775.1"
                     /translation="MSELRCIIIEDEPLAVKVLVEYISQVPFLKLQATFKDALLATDW
                     LRENGTDLIFLDIHLPRLKGLSFLKTLGHPPAVILTTAYHQYAVEAFNLNVTDYLLKP
                     FEFERFLVAVNKVKTHQAVLLNKRDHPETRDYLFLNVQKKKVRIAFSDIVYIESQREY
                     IKVVTLKDAYLSKMSTHEIESLLPPRQFRRIHRSYIVSLDKIEAYTAEAVEVNGVSIP
                     VGRGYRESLEGL"
     gene            6751..7728
                     /locus_tag="PKM27_15765"
     CDS             6751..7728
                     /locus_tag="PKM27_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR08776.1"
                     /translation="MLKKLLILLFALILFVLIGILAFFGPYYARVNYHAPNPAKGYHA
                     GFYLYISPGAKKTGLQGGPVTLLVQPNNTGTISDNAKVHQKGAWMTGFERHKIANDLN
                     VVLLVPAFIRPAEDWHIYTQALDRDAMVTKRKDLIRIDLQLLAMIDEARALLKEDGIE
                     SNEKFLLQGFSASGMFANRFTILHPEKVLAVVAGSPGGWPIAPVNYYKEVLLAYPAGI
                     GDLEVLTGKAFDSLSYVGIPQLIIMGSADDNDSLDFTDGWDKEAASIVDGFFGKDPLS
                     RWPHAEALYRQAGAKVKFVLVEGVGHDRKKLQSYGVEFFADILNPQNPD"
     gene            complement(7706..9100)
                     /locus_tag="PKM27_15770"
     CDS             complement(7706..9100)
                     /locus_tag="PKM27_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNR08777.1"
                     /translation="MIPCIAILDVGKTNKKILVFNRDYELVHEQIDQIPETQDEDGFP
                     CEDLQALSQWALNTMESLVRNGGFDIKAINFSAYGASFVLLGEEGSPVAPLYNYLKPI
                     DQDLLEDFYDQYGGKASLLRETASPALGNLNSGIQLYRLKKWNPEKFSRIKTALHLPQ
                     YLSYLFTRKAVSELTSIGCHTMLWDFERDQYHRWVHEEGLMKWFPGNEPADSAFPIRL
                     ADKNVLCGIGLHDSSAALIPYLKTIPDPFILLSTGTWSIALNPFNQEALSAAQISDDC
                     LFYFSYEGRPVKAARLFSGHEHEEEVHRIADCFDCNPEFYKNLRFDSGYWQALSENYV
                     EQPDSVDEHGLKLSGFRDRFVQFFEDETEAYYTLIADLVGLQVRHLRLIQDEQAIKQI
                     YVDGGFSKNLIFMHLLAERLPDSRVFASTVAQASALGAALVLHHHWNGGELPAGLVKT
                     DHVGINPINRDFAD"
     gene            complement(9182..>9451)
                     /locus_tag="PKM27_15775"
     CDS             complement(9182..>9451)
                     /locus_tag="PKM27_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HNR08778.1"
                     /translation="LQSVEAILQAYAKALLIDRASLKTAQMQNDTVRCGEILQEAYQT
                     DVRPLLAEARLRSGGALDPIQAYRDLGVRQKLVEARGHGSVATGL"
     assembly_gap    9452..9551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9552..10611)
                     /locus_tag="PKM27_15780"
     CDS             complement(<9552..10611)
                     /locus_tag="PKM27_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNR08779.1"
                     /translation="MKINPSDLEQPNEKLLKTHKQKLEWFNMFSDQTENIIQKLIDFQ
                     IAIPSWALGTGGTRFGRFSGGGEPATLEDKIDDVGLIHALNRSSGAISLHIPWDIPQD
                     YQKIKNRAASLGLAFDAVNSNTFQDQEGQPLSYKFGSLQNTDPAIRRQAIDHNIEVIR
                     HGVELGSKSITVWLSDGSSYPGQLNFREAFQRTLEGLKTIYEALPPDWKMFVEYKAFE
                     PNFYSMTVGDWGQSMLYAQKLGPKAFTLVDLGHHLPNANIEQIVALLLMEGKLGGFHF
                     NDSKYGDDDLTVGSINPYQLFLIFHELIDGMDTRRMDHARDLGWMIDASHNIKDPLED
                     LLQSVEAILQAYAKALLID"
     assembly_gap    10777..10876
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10877..11747)
                     /locus_tag="PKM27_15785"
     CDS             complement(<10877..11747)
                     /locus_tag="PKM27_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR08780.1"
                     /translation="MSRYTLFFFLITGLTCRDAKPAQLTRNQEFPWSFRPNILWLVAE
                     DLSPVIPPFGDSTIETPNLSRLAAEGIRYPMVFSPSGVCAPSRAALAMGMYPSRFGAQ
                     HMRTGFWPGGRPRPEQVKAQASSFPEGLPQYEAMPPPEARMHSEYLRMEGYYCSNRFK
                     QDYQFVAPVTAWDDCSRTAHWRNRKDGQPFFSIFNFEVTHESQIWTKASDSLWLDSTL
                     NIPVPPYLPNTPVALRDVRRMYSNIREMDAQIGKVLAELEEDGLLDSTIIFWYGDHGG
                     PLPRQKRLCYDSGL"
     gene            11843..12220
                     /locus_tag="PKM27_15790"
     CDS             11843..12220
                     /locus_tag="PKM27_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08781.1"
                     /translation="MKRSIFFIITAVVAILFGGMMLLTPAMTSENFGLETTPESTLLF
                     RSLGGMVLSLGLLNFLVRNEPDSKPLQAVLMVNMVSHIISMGNDFYGASQGILEFSKL
                     AGGMVAHVFIVVGSAFYLLKMKS"
     gene            12260..12628
                     /locus_tag="PKM27_15795"
     CDS             12260..12628
                     /locus_tag="PKM27_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR08782.1"
                     /translation="MTNSINWFEIPVKNFERAKAFYSALFDAEIQQMPHPTLKYGILP
                     GDMQNGVTGGIVEGDGFEPSMTGALIYLNGGDDLSIPLSKVEKAGGKIVLPKTSIGAN
                     GFMAYFIDTEGNKVALHSMG"
     gene            12933..13355
                     /locus_tag="PKM27_15800"
     CDS             12933..13355
                     /locus_tag="PKM27_15800"
                     /inference="COORDINATES: protein motif:HMM:NF017907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BphX family protein"
                     /protein_id="HNR08783.1"
                     /translation="MNFLKWWLRIVGLFYVLQFVMVAIVRAPIRVQGPAGALEDAAAG
                     NPMARFLVDTWVTFGLEVGAIGMVLLLASRVPETARILVWLIIGIEIGRGLIADSYMI
                     SRGHPLPTLLIWLVLHSIVILTGWMALQKSKTETDKIN"
     gene            13543..13935
                     /locus_tag="PKM27_15805"
     CDS             13543..13935
                     /locus_tag="PKM27_15805"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNR08784.1"
                     /translation="MKSEIIEYHQRLTPADQAICDLLASVILETLPEADYKIWHAHPV
                     WFLEGNPVVGYQKLKDSVRLLFWSGQSFEEDQLDKEGKFKAAEIRYTGKEQIKVADLK
                     RWLEKARAIQWDYKNIVKRKGRLERLKG"
     gene            14009..14386
                     /locus_tag="PKM27_15810"
     CDS             14009..14386
                     /locus_tag="PKM27_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08785.1"
                     /translation="MKSKVYNLLLILTSLFGYLEWGGNNHQFLFEAEAEIFSRLINDP
                     LSVIHPLVLLPLAGQILLLITLFQRKPSKILTFIGMGGLGILLGFMALVGVLGLNYKI
                     VLSVLPFLTIAVMTILFYRKTHS"
     gene            14383..>14729
                     /locus_tag="PKM27_15815"
     CDS             14383..>14729
                     /locus_tag="PKM27_15815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR08786.1"
                     /translation="MKIIALLRCDHLKKSISFYTEILDFTLKFPDEAENEWVVDLING
                     EAEILLSSIDGTPRNSVYIRVEDVDALFNKYIRRGLIVPNNPDSPVHQSPIDQTWGLR
                     EFYVNDPDGNTLR"
BASE COUNT         3842 a         3407 c         3512 g         3768 t
ORIGIN      
        1 ggaagattat ttaccaaacc taaagtgaag tcgcttaaag accaccgtgc ttcgtactaa
       61 aatgtgaatc cattattcaa acaaatttac aaagaaccga ttaagtcaga ctgcattgac
      121 cgagtagcgc gtaaacaaat ttgaaatgaa gttctggtga ggaattaatg gaacgaggat
      181 ttaaactgat gtaattttag cagaagaatt taactgttgc aggagtctgc cagtattaaa
      241 atgtggcgag tacgtcagcg gttgttcatg agagttgttg ataaccgaat ctgaaggaaa
      301 agatgagaag gctaagaaag atgtccattc cggaaaaaca caccaatacc aaggccacca
      361 atgccggcac acaggccacc cgtaggttta cacatgtatg gttttttcca acaatagttg
      421 gtctataata gcgcgtgagg caccattata gaattagaaa tacaggaaat agtcttcgtt
      481 ccacaatcgt attatatcgt ataaatacca atctggaccg ataagtacat attttttcga
      541 tccttaccgt attttatagt atcattataa accaaatgat tcggctgatg aaaagcgaag
      601 gccaacatgg ggcttttaag ccggctatac aagaaagttc agcccgattt cggaagccaa
      661 ccggtagtcc gcaaacttaa aaagaaaacc ctaaatggaa attgaattaa aaagtttaaa
      721 ccctaagttt gtaggactgt tcaggcaaac cttcgcattt accgaggaag aatttgcttt
      781 gtttttatct ttttttcagt tcaaaaccat ttgcaaaaaa gagtattatt tgagagctgg
      841 cgaattatgc aaagcaaagg cctatctcaa taagggttgt gcaagaaact ttgtggtgga
      901 cgaaaaagga cacgagcgga ttttattctt tccgtttgaa gactggtggc tgggtgactt
      961 cgatagttat tattcgggta aaccggcaac aaactatgtt caaatgctcg aagattcaga
     1021 actactggtc atctcaaaag agcattttca aaaggcagag caggaaatac cgaaacttaa
     1081 acaatggtat tcttccaaaa tgctcagttc cgcaagtgct gcccgaaaca gaattgaaga
     1141 aatgaaaacc ctgtcggcag aagaacttta tctcaaatta ttggagaagc aacctcacat
     1201 ttttcaacga attcccttgc agtacattgc ctcttatctc aatattgaac cccaatcgct
     1261 cagccgcatg aggaaacggc ttttaaaaaa ccattaaaac tcttttactg cttgtttttc
     1321 ttaacccggg ttaagtgttt tgttttttac accaggcatc tttgtatccg gtaatttatc
     1381 aatcactaaa aacaaaaaca tgactacatt aactacaact cagagcagag tgctcaaacc
     1441 aaaggagggg ttgaagctgc aatccggccc cggccgcgag cttatttata aagttaccgg
     1501 cgaagacacc ggtggcgcat ttgactattt tatagttgaa gtggcgccac acggaggccc
     1561 accactgcat gtgcaccata agcaggagga aaccatccat gtgcttaaag gcaaatttaa
     1621 agtccggatt ggtgatgaaa tattgtactg caatgaaggg gactttgtct atctgccctc
     1681 aaaagttccg catgccttcc ttaacctgac ggatgagcag gctgagatca ttgtcgtcta
     1741 tacacccggt ggaggccaca aattttatga agaactagga ccgcttatgc gcaatggttc
     1801 gccagataaa aaggtagtcg ctgaactatt tgaaaaatat gaaatgacca tacttggtcc
     1861 tccattaaag caggattgag ccaaagaacc tgtatttgat ttggattcca gaagcaaaca
     1921 ttagccaatt ggctaaaact ggaaacctaa acccaggatt acttcgacaa tatgtttctg
     1981 gcacaaaaaa aaatcagaat cacagataga aaaagttatg aaagcagctc cctgatcatg
     2041 acctcatctt caattataaa ccgattgccc ggatcggtct tttcaatgcc cgttttgaca
     2101 aaaccgtatt tttcatagaa acgaatggct ttacggttga ggtagtagac cttaagtctt
     2161 aaggcggtct gatgcttttg cgaggcaatt cctgtaaggg catccaggac ctgtgtccca
     2221 atgccattcc cctgtaaggc aggggagaca tataatttgt ggatcatcat tcgcggttgt
     2281 cctctatagt tgatttcata ggaggcaaaa cccacttgga ttccttcaga ttctgccagc
     2341 aggtatacat gcccgttttc attcatctgg ctttggatgg tatgcctgct gaatagcttt
     2401 tccaggagat agtggacctg ttccatcggc atcagggtcc cgtaggtgac cggccaggtt
     2461 tcaaaagcaa tgttccggat cttttgccag tcttccattc cggccttttt aatgtcgatc
     2521 atcgggttaa aatcatcggg atgggtccaa attagaaaaa taattcgacc gcctgcaata
     2581 tttaaaaggc tcaaggactc acattctcat gcgtctctgc ttttttcctg atgccaatca
     2641 ataacaacca taaggtgatg gccaattctc cgaaaaaaaa cgggaaggcc caaacgcgca
     2701 tctgggcttt gtacgacgga aagagtaaaa gggtaaacca ttccagtata taggccagtc
     2761 cgttgagcat cagaaaaagg ccgaggatcc ggggaataaa acccgacttc cagaccagcc
     2821 aggcaaaggg gaaaagccaa agcccccaaa agaattctaa agaaagggtt ccctgcttgc
     2881 cgatctccaa taggatcagg gccacttgct cctgcttccc tggttccagg aatttaagcg
     2941 cttctccctt caggatcatc agcgaggtga gtggaaatac ctcgagcacg aatccaatgg
     3001 ggatttgcag gacgatcagg atcaccatga gcctggcttg cttgctgtca accggctcaa
     3061 acaaacggta caaggccaat acaacgaaaa gaaataacag tccactgatc atcccgttga
     3121 ccaaaccggt ccggaacaaa aattcattgg ccaggatgtt tttggctgtg gtcgcggcat
     3181 ccccgccctg tggaattttg gaaggaagat aaatcaggcc gtaaagtccc gaaaagacca
     3241 ggaaaagata aaggaggcca gccagcctgg cttttctttt taatgcaatg ccattgaagt
     3301 ccatgaccaa ggcctgcatt tatttatttc cggaaaattt atccatggga ccgggatgat
     3361 cttcacggat gagtttttgg accagctttt ccgccacttt ttttcccaat tcccgggcgg
     3421 cttcattgtc gctgcggaaa tgaatgcccg cctggaagcg ggactcggca gcctctgctg
     3481 cttttttcca ataacgttcc ttttcttccg gaaaaaaata agacatcagg ccggcctcta
     3541 ctgccgtgat ggccgcatgt ccggacgggt agccgggaaa aggcggagtc accaggatgg
     3601 ccggactgaa agtggtatca tattgattgg gccgtatgcc ccaataggtg tacttggcat
     3661 cccaacaggc tacaaaacca tcgtagagtc ctacacccat cgccgcatgg atataagcgg
     3721 ccaggggtgg gttgaggtgc aggttgtatt cgaatatttt tttcttcagt tcctccgcaa
     3781 accaatccgc attggcccat tgaaaggcat tgctgaggga acggaagttt tgtttgtaat
     3841 gcctcagctc ttccatctct ttggcataat ccggcggagg acccggacga aactcgtccg
     3901 gcccggagag gactactgtt ttgtttttgc ccgccatagg cagaagggcg aatctccccc
     3961 gccagtattg ggggccttcc ggtatcttgc catcccaagt ctctttgcaa acaaaatcac
     4021 tggttcgttc aatttcttcc ccggcgatcc gcatgcccag ctcaaagcct gccctggtat
     4081 ccgaaggaaa ggcgacgcct gctgccaccc tggagtccat caactgccgg gccagcttcc
     4141 ggacggaatc ggccatcgcc ggataaaatg ccgaaaagac cgacaccgcc acaccggcag
     4201 ccaccgaatg ttcgcagggg aaggaaggac tttcgggcgg aggaatacga atattgatcc
     4261 tccggtccgc ttcgaaaggc cttggacggt gatgggtgta tttgctgtcc caggccgcaa
     4321 ccgtcgcgtc ataaatggcc gtgccgagca acatgttggc cagggctccg tacctgcccg
     4381 tatccaaggt ccagagccgg gtcatcatct cctgccagcg atagcaggga ggaccggcat
     4441 tccaataatg gatttgtagc cgggtggttt cgtccgcttt ttgctggatg gacaggacct
     4501 gggcaatttc atccttgtaa gatccgggcg ccggcaggcg gtaatccctg ccggaagaaa
     4561 taaaccaggt cttccagagg ccggccgagg ggtcaatctg tccaacaagg tatccctgga
     4621 tgaaaaaagt gataaagagg gtttggaata aacggatcat gatttcttgt atttatcaca
     4681 atactattcc cgaaaagaat gtggagtgga gctccgtcaa caaacaaccg taccgggtat
     4741 accgaaggtc cggcaagacc catgggcctg atcaaaaaac cggccgaatc attcccttaa
     4801 atttctaatc aactgccggc caattcataa tgaatgtttc atcaagccgg atctttcatt
     4861 cgttgaccct tttggtccat tggcatacgc agttattctt tccgtagacc gggaccggag
     4921 gagattaaga aatcacgacc tttatactat caggggaaat gaaatggccg ctcgatatca
     4981 aaaagatcaa attttacctt tgggtcgggc tcgcttattt tggcctctgg tcgatcggca
     5041 acctggtggg ttaccgggat accttatggg tcaggacggt aaatgatcta tggagggtcc
     5101 tctatatcat cgccataaac ttttcttttt ttgaatacgc ccttcccttt attctggcaa
     5161 agagaaagtc tgttttatac aatatcctat ggggcctcct gatagtttgg attttcctga
     5221 tgttgtggtc tttcggcctc tatggctgga gggccatcgg gatcgccctg ggcatctata
     5281 cggaactgac ccgcttcaaa accgttgacg aaggcgtcag ctaccacttc ggatacagtg
     5341 tgggttccat cctttttttc ggactggtgc ggcatgtgtt tcaatacatt cagctcaggc
     5401 aatcggccca gcaactgcgc attgaaaaac aacaggccga actgaattac ctgaaatcgc
     5461 agaccaaccc gcattttttg tttaatacgc tcaacaatat ctattccctg gcccgggaca
     5521 aatcggacct ggcaccggaa tccatcatgc gattgtcaaa gatccttcgt tatatgctct
     5581 acgaaaccgg gggtgaatat atcgccatcg agcaggagtt aaaaatcatc ggcgattata
     5641 tcgagctgga gaagctgcgc tacgatgaat cgctgcgggt caattttaat tatgatattg
     5701 aggatatgaa gcaggccctg cccccgctgt tgctgatccc tctggtggaa aacgctttca
     5761 agcatggggt atccgagacc cgttcacagc cctttgtaga tatccatctt tcggtaaagg
     5821 agcgccggtt gaattttggg gtaaaaaact cggtggatcc gttttccggc gggaaagccc
     5881 ttaaagaaaa tatcggcctg gccaatctcc ggcgccaact cgaattgctt tataccgatt
     5941 accggcttgc ggtgaatcag ggagattcca tgtttacggc tatcttatac attaacctgg
     6001 caagccatgt ctgaactccg gtgcatcatc attgaagacg aacccctggc ggtaaaagtc
     6061 ctggttgaat atataagcca ggtccctttt ttaaaattgc aggctacctt caaagacgcc
     6121 ctcctggcta cggactggtt gagggaaaac gggaccgacc tgattttttt ggacatccac
     6181 ctgcccaggc tgaagggact ttccttcctc aaaaccctgg gccatccccc ggccgtgatc
     6241 ctgactacgg cctatcacca atatgctgtg gaagcgttca accttaatgt caccgattac
     6301 ctcctgaagc cttttgaatt tgaacgcttc ctggtggctg tcaacaaggt caaaacccac
     6361 caggcagtcc tgcttaataa acgggatcac ccggaaaccc gggattatct ttttctgaat
     6421 gtccagaaaa agaaggtcag aatcgctttc tccgatatcg tttatataga aagccagagg
     6481 gaatatatca aggtagtgac cctcaaggat gcttatctgt ccaagatgag cacccatgag
     6541 attgaatccc tcctgccccc caggcagttc aggcggatcc atcgttccta catcgtctct
     6601 ctggataaga tcgaggccta taccgccgag gcggttgaag tcaatggagt gtcgataccg
     6661 gtgggccggg ggtataggga gagcctggag ggtttatgag gaggcgtttc gttattattg
     6721 ggtatatttt ttgaatccac ccatcagatt atgttaaaga aattattaat tctcttgttt
     6781 gccctgatct tatttgtact gattggcatc ctggcttttt ttggacctta ttatgcaagg
     6841 gttaattatc atgcgccaaa cccggccaaa gggtatcatg ccggctttta tctctatatt
     6901 tctccgggag caaaaaaaac aggcctacaa ggagggcctg tcactttact tgtccaaccc
     6961 aataataccg gcaccatttc ggataatgca aaagttcatc aaaaaggtgc ctggatgacc
     7021 ggctttgaaa ggcataagat agccaatgac ctcaatgtgg ttctgctggt accggcattt
     7081 ataagaccgg ctgaagactg gcatatttat acccaagccc tggaccggga tgcaatggtc
     7141 accaaacgaa aagacctgat ccgcatcgat cttcaattgc ttgccatgat cgacgaggcc
     7201 cgggccttac ttaaagaaga tggaatagaa tcaaatgaaa aattccttct tcaggggttt
     7261 tcggcgagcg gaatgtttgc caaccggttc actatcctcc atccagagaa ggtactggcc
     7321 gtggtggcgg gatccccggg cggatggccc attgcacccg taaactacta caaagaagta
     7381 ttattggcct atcctgcagg catcggcgac ctggaagttt tgaccgggaa agcttttgat
     7441 agccttagtt atgtgggcat ccctcaactc atcatcatgg gatcggcgga cgacaacgat
     7501 tcccttgact tcaccgacgg atgggacaaa gaggcagcgt caatagtgga tggatttttt
     7561 ggaaaagatc cgctgtccag atggccgcat gcagaagctt tgtacagaca agccggagca
     7621 aaggttaagt tcgttctggt tgaaggggtc ggtcatgacc gtaaaaaact tcagtcctat
     7681 ggcgtggagt ttttcgccga tatccttaat ccgcaaaatc ccgattaatc ggatttatcc
     7741 ccacatggtc cgtttttacc agcccggcgg gaagctctcc cccattccag tgatggtgca
     7801 aaaccagcgc cgctcccagg gcggaggcct gagccacggt ggaggcaaat acacgggaat
     7861 ccggaagccg ctcagccagg agatgcataa aaatgaggtt ttttgaaaaa ccaccgtcca
     7921 cataaatctg ttttatggct tgttcatcct ggatcagtcg aaggtgcctg acctggaggc
     7981 ccaccaggtc cgcgataagg gtataataag cctcggtttc atcctcaaaa aactggacaa
     8041 agcggtccct gaaaccagaa agtttcaggc cgtgttcatc cacggaatcc ggctgctcca
     8101 cataattttc tgaaagagcc tgccaataac cggagtcaaa ccggagattt ttgtaaaatt
     8161 ccggattaca atcaaagcag tcggcaatcc gatggacctc ttcttcatgc tcatggcccg
     8221 aaaacaaacg cgcagctttg accggcctgc cctcataact gaaataaaac aggcagtcat
     8281 cggaaatttg tgcggcactc agggcttcct ggttaaaagg gttgagggca atgctccagg
     8341 tcccggtaga caatagaata aaaggatccg ggatggtctt caaataagga atgagggccg
     8401 cggaactgtc atggaggccg atcccgcata atacgttttt atcagccaac cggatgggga
     8461 aagccgaatc cgccggctca tttccaggga accatttcat caacccttct tcatgaaccc
     8521 agcgatgata ctggtcgcgt tcaaaatccc agagcatggt atgacaaccg atgctggtca
     8581 gttcggagac tgctttgcgg gtgaataaat aactaaggta ttggggaagg tggagggctg
     8641 tttttatccg gctgaacttt tccgggttcc attttttcag gcgatacaac tggatacccg
     8701 aattgagatt acccagtgcc ggggatgccg tttcccggag caggctggct ttgcctccat
     8761 attggtcgta aaaatcctcc agcagatcct ggtcgatggg tttcaggtaa ttgtaaagcg
     8821 gggcgaccgg actgccttct tcaccgagca aaacaaaact agcgccgtaa gcggaaaagt
     8881 tgatggcttt gatgtcaaag cctccgttcc ttaccaggct ttccatggta ttcaatgccc
     8941 attggctcaa ggcctgtaaa tcctcacagg ggaaaccgtc ctcatcctgg gtttcgggga
     9001 tctgatcgat ctgctcatgg accagttcat agtcccggtt aaataccagg atcttcttat
     9061 ttgtttttcc tacgtccagg atagcgatgc aggggatcat ttttcaatta aaaattaaag
     9121 gaattaagaa ttaaaatccc acctcattca tttttaattc tttaattatt aattaattta
     9181 attacaaacc cgtcgccacc gacccatgcc cccgcgcctc caccagcttt tgccttaccc
     9241 ccaaatcccg gtaagcttgg attggatcca aggccccgcc cgaccgcaac ctcgcttcag
     9301 ccagcaatgg cctcacatcg gtctggtaag cctcctgcag gatctccccg cagcgcaccg
     9361 tatcgttttg catttgggct gtctttaaag aagcgcggtc gatcaatagg gctttggcat
     9421 aggcttgcag gatggcttcc accgattgaa gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nggtcgatca atagggcttt ggcataggct tgcaggatgg cttccaccga
     9601 ttgaagcaga tcttccaggg gatccttgat attatgggac gcgtcgatca tccaacccag
     9661 gtcgcgggca tggtccattc tccgggtatc catgccgtcg atcagttcgt gaaagatcag
     9721 gaatagctgg taaggattga tgctgcccac cgttagatca tcatccccgt atttgctgtc
     9781 gttgaaatga aaacccccaa gtttgccttc catcaggagc agggcaacga tttgttcgat
     9841 attggcattg ggcaggtggt ggcccaggtc caccagggta aaagccttgg ggcccagctt
     9901 ttgggcatag agcatggact gtccccaatc tcctacggtc atggaataaa aattgggttc
     9961 aaaggctttg tattcgacaa acattttcca gtcgggaggc agggcttcat atatcgtttt
    10021 taatccttca agggtccttt gaaaagcttc ccggaaattc aactggccgg gataactgga
    10081 tccgtccgac aaccataccg taatggattt ggatcccagc tccaccccgt ggcggatcac
    10141 ctcgatattg tggtcgatgg cctgccggcg aatggcggga tcggtattct ggagggaacc
    10201 gaatttataa ctgagcggct gtccctcctg gtcctgaaaa gtattggagt tgactgcgtc
    10261 gaaagccaaa cccagcgatg ccgccctgtt tttgatcttt tgataatctt gaggaatgtc
    10321 ccaggggatg tgaagggaaa tggccccgct ggaccggttc aaagcatgga tcaggcccac
    10381 atcatcaatc ttatcttcca gggtagccgg ttcgcctcca cctgaaaaac gtccaaaacg
    10441 ggtaccccct gtgcccagcg cccagctcgg tatggcgatc tggaaatcga tcagcttttg
    10501 gataatgttt tccgtctgat ctgaaaacat gttaaaccat tccagttttt gtttatgggt
    10561 cttcaataat ttttcattgg gctgttcaag gtcggatgga ttgatcttca tgatcctttc
    10621 tgtattttat tcagcgatta cgttactggg taaatatcct atgcggtggg cgagtgcttc
    10681 gactttattt cccggcggca aaatgaaagg ttcctggtat acttgccagg atttggaggt
    10741 gtctgaagat gccaggattt tgtatcctat ggaagcnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnttaa tcccgaatca taacaaagcc tcttctgccg gggaaggggt
    10921 ccgccgtgat ccccatacca gaatatgatt gtactatcca ggagtccgtc ttcctccaac
    10981 tccgccagca cctttcctat ctgagcgtcc atttcccgga tattggaata catgcggcgg
    11041 acatcgcgca aggccaccgg ggtattgggc agataaggcg gtacgggtat gttcagggtc
    11101 gaatccaacc aaagcgaatc gctggccttt gtccagatct gggattcatg ggtcacttca
    11161 aaattaaaaa tggaaaaaaa cggttggccg tctttccggt ttctccaatg ggccgttctg
    11221 ctgcaatcat cccaggcggt caccggcgcc acgaactggt aatcctgctt gaagcggttg
    11281 ctgcaataat atccctccat gcggaggtat tcgctgtgca tgcgcgcttc aggcggaggc
    11341 atggcttcat attggggaag gccttccggg aaagaagaag cttgtgcctt gacctgttcg
    11401 ggccttggtc ttccccccgg ccaaaaaccg gtgcgcatgt gctgggcgcc aaaacgagag
    11461 ggatacatgc ccatggccag tgcggcccgg ctgggcgcac agacaccgga aggactgaat
    11521 accatgggat aacggatgcc ttctgcggcc agacggctga ggtttggggt ttcgatggtg
    11581 gaatctccaa agggagggat gaccgggctg aggtcttcgg caaccagcca gaggatattg
    11641 ggacggaagg accagggaaa ttcctggttt ctggtcaact gggcgggttt agcatcgcga
    11701 caggtcaggc cggttatcag gaagaaaaag agggtataac gggacatggt gaaaggatgg
    11761 gtaatttatg gaattaacgg gaatgacagg gtggaaacgg aacagttttc tttcgtaatt
    11821 tcatcaataa aaaccaaaca acatgaaacg ctcaattttc tttatcatca ccgctgtggt
    11881 agctatactc ttcggcggca tgatgttgtt gaccccagcg atgacatcag aaaatttcgg
    11941 gcttgaaacc actccggaat caacactcct tttccgttca cttggtggga tggttttatc
    12001 gcttggcctt ttgaattttc ttgtccgaaa tgaaccggat tcaaaaccgc ttcaggctgt
    12061 tcttatggtc aatatggtat ctcatatcat cagcatgggc aatgattttt acggtgcaag
    12121 ccagggtatt ttagaattca gtaaattggc tggcggaatg gtcgctcatg tttttattgt
    12181 tgttggttcg gccttttatc tcctgaaaat gaaatcatag gatgcattat tgcatcagta
    12241 taagtaaatc aaaaactaaa tgacaaattc catcaactgg tttgaaattc cggtaaaaaa
    12301 tttcgaaagg gctaaagcct tttattcggc attattcgat gcggaaattc agcaaatgcc
    12361 acaccctaca ttaaaatatg gcatattgcc gggagacatg caaaacggtg ttacgggggg
    12421 catagttgag ggtgacggat ttgagccctc catgactggg gccctcattt atcttaatgg
    12481 cggggatgat ttgagcatac cgctttccaa agtagaaaaa gcaggaggta aaatcgtttt
    12541 accgaaaacc tcaattggcg ccaatggttt tatggcctac ttcatagata cggaaggaaa
    12601 caaagttgct ttgcattcga tggggtgaaa tgggtttctt tttgcactct catctggcta
    12661 ttgtttagtg gtctttgcat cttcactcaa gcccacattg cttttaaacc atgcgatgga
    12721 taattataaa cccggacccg gactattgac ttccctatct tttactgctg aatttacaag
    12781 ttgaaatttt cagcttatcc tcgtataatt ttcaaagaaa ttgcggaaac gggttggtta
    12841 ggtaaatccg gaaataaatg aacatccgga ttccggtttt tatttcttaa tttcggtaaa
    12901 agccggtttg gctgtttaat aaacacaatg ccatgaattt tctcaaatgg tggctccgga
    12961 tcgtaggctt gttttatgtt cttcaattcg tcatggtggc tatagtcagg gctcccatcc
    13021 gcgtacaggg ccctgcagga gccctggagg atgcggcagc aggcaatccc atggccaggt
    13081 ttttggtaga tacctgggtt accttcggcc ttgaagtcgg ggcgatcggt atggtcctct
    13141 tgttggcatc ccgggtgccg gaaacggccc gcatcctggt ctggttgatc atcggcatcg
    13201 aaattggaag aggcctgatc gcggatagtt atatgatcag ccggggacat ccgcttccca
    13261 ccctgctgat ttggctggtc ctgcactcca tcgttatcct gaccggttgg atggccctgc
    13321 agaaatccaa aacggaaacg gataaaataa attgaaagca cctcatgtat tcttcctgtt
    13381 tccaataggt ataaaactgt tggagacttt aatcaggtcg tgagctgaaa ggatcaaaat
    13441 cataacagcc cccgatcggg agatgagagt catatgccct aattatttgg taaatttctg
    13501 gccattaggc aaccgtttaa atacctactt tttaaaaaga atatgaaatc cgaaattatt
    13561 gaataccatc aaagactgac ccccgcagac caagccatct gcgacctatt ggcatcggtt
    13621 atcctggaaa ccttgcctga ggcggactat aagatctggc acgcccatcc cgtctggttt
    13681 ctggagggca accccgtcgt cggctaccaa aaactgaagg acagcgtgcg gctgctcttt
    13741 tggagcggcc agtcctttga ggaagaccag ctggacaagg aaggaaaatt caaggccgcc
    13801 gaaatccgtt atacgggaaa ggaacagatc aaggtggccg atttaaaacg atggcttgaa
    13861 aaggcgagag ccattcaatg ggattataaa aacattgtca aaagaaaggg ccggctggag
    13921 aggctgaaag gatgatccat tcgtgaacat ccatttccta tacggcaggc cgggttccct
    13981 ggacccatcg aagaaataca ttcatttcat gaaaagcaaa gtttacaacc tgttgctgat
    14041 cctgacctcc ctgttcggtt acctggaatg gggcggtaac aatcatcagt ttctttttga
    14101 ggcggaggcg gaaatctttt ccaggctgat taacgatccc ctctcggtca tccatcccct
    14161 ggtcctcctg cctttggccg gtcaaatcct gttactgatc accttgttcc aacggaaacc
    14221 atcaaaaatt ctaaccttca taggcatggg aggcctgggg attttgctgg gttttatggc
    14281 tttggtgggg gttttgggtt tgaattataa aatcgtcctt tccgtattgc catttttaac
    14341 aatagctgta atgactattc ttttttatag aaaaacgcat tcatgaaaat aattgcactc
    14401 cttcgatgtg accatctgaa gaaatccatt tccttttata ccgagatcct ggattttact
    14461 ttaaaatttc cggacgaagc ggagaatgaa tgggtcgtgg atttgatcaa tggggaagcg
    14521 gaaatcctgc tctccagcat agatggaacg ccaagaaatt ctgtatacat aagggtagag
    14581 gatgtcgatg ccctgtttaa taaatatatc cgacgtggct tgatcgttcc aaacaatccg
    14641 gattcacccg tacaccaaag tccaatagac caaacctggg gattgcgtga attttatgta
    14701 aatgatccgg acggaaacac cctgaggtt
//
LOCUS       DAOQXT010000108        14703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4552_length_14703_cov_7.520549, whole genome shotgun sequence.
ACCESSION   DAOQXT010000108 DAOQXT010000000
VERSION     DAOQXT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14703
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4552_length_14703_cov_7.520549"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1021..2208)
                     /locus_tag="PKM27_15820"
     CDS             complement(1021..2208)
                     /locus_tag="PKM27_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR08787.1"
                     /translation="MNLFDVYPLYDIEPVRGRGCYVYDQKGVEYLDFYGGHAVISIGH
                     SHPHYIQRITKQLQKLGFYSNSIINSLQKELADKLGKISGCEDYQLFLINSGAEANEN
                     AIKLASFQNGRKKVISFKKSFHGRTSAACNVTDDPKIKSEINKTFDVEFCPLNETGRL
                     EELLESREYACVIIEGIQGIGGVYVPDATFLRELQTLCRKTETLFILDEIQSGYGRSG
                     KFFAYQHADDIRPDLITVAKGMGNGFPIGGVLIQPGIKARHGMLGTTFGGNHLACAAA
                     LAVLEVMEEDDLVSHSAEVGEYLMAEMTQIPQVREVRGKGLMVGVEFDFPVADLRKQL
                     VFKERVFTGSAADPNTLRLLPPLSIQANQVDQLTEKLRSVLRTLSDKTPGYRPVAKAS
                     SKS"
     gene            complement(2258..3511)
                     /locus_tag="PKM27_15825"
     CDS             complement(2258..3511)
                     /locus_tag="PKM27_15825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR08788.1"
                     /translation="MAQTVLRLDDAIEIALSQNPNILISSNEKEIAQVNNNWWAAGRI
                     PTVGLNSTFNNSITNLNQKLNNGTVIERNGARNSTINVNGQFSYRIYGGKRMYIIKNR
                     LDLEEALADEVLKQDINQTVFDVINRYIDIVRLKSQLDAVRETISFFEERSKLSQVRF
                     EIGTAGKNDYLQSQVDLNVQRTSELNILNSIELAKMELNRVMVRDPFTAFEVEDIPAP
                     EILPSREEMMQAIDAQNPQITALRFNQKILTQLREEILATQKPSVFANASANFNRNSS
                     TAGFNLFTMTYGPQAGLSLSVPLFAGPIVRQQVKVNDLQYKNQDLQIQNVKNDLTSLA
                     ANAYQNYENANRQIGLEEQNLAIIREHNAISMERFRKASITTVELRQAQLNLIEAQNR
                     IINSRFILRQSEAQILYVMGRLVEQ"
     gene            complement(3620..6688)
                     /locus_tag="PKM27_15830"
     CDS             complement(3620..6688)
                     /locus_tag="PKM27_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR08789.1"
                     /translation="MNISEVSLRRPVFAIVLNLIILLFGIIGFKFLGVREYPAIDPPF
                     INVRCTYPGANADIIESQITEPLEKAINSVPGIRNVTSSSSQGNCNINVEFELGVDLE
                     EAANDVRDKVSQAARSLPADLDAPPVVSKADASSDPILSMTVQSPNRNQLEITEFANN
                     ILVERLQTIPGVSSIQIWGEKRYAMRIWMDPDKMNAFGITAKDIQVALQRESVEIPSG
                     KVSGEAVELSVRTFGRLNTEEEFNNVIIRSSETGILRLGDVAEAVLGPENEESVFRES
                     NIPMISLAIIPQPGTNYVNISDEFYRRVDQLKRDVPEDVKLGIALDSTKFIRKSLDEV
                     KETLILSLSLVIAIIFFFFRSWGIALRPLLDIPVSLIGAFFIMYLFGFTINVLTLLAI
                     VLATGLVVDDGIVVTENIYKKIEAGMNKWQAAREGSKEIFFAVLATSITLAVVFIPII
                     FLQGFVGRLFREFGIVVAGAVLISALVSLTLTPVLNVKMATSAHHSWLYRVTEPFFKG
                     MENGYRRSLSAFLKVRWMAFVIVLACGGVIYFIGSKIKSELAPMEDRSVFRIQVTAPE
                     GTSYEAMDRNIVAITDYVLEQVPEKTTVMSMTAPGFTGSGSVNTGFVRVVLSDPHDRE
                     RSQAQIVQAVSKGLPQFTDVRMFAAQDQTIQVGRRFGGQPVQFVVQNNNFEKLKEALP
                     RFLEEIQKSQILVNADVDLKFNKPELRIQIDRLKASELGVSIGDLSEALQLALSNRRL
                     GYFIKDGKQYQVIGQVMRNDRDDPNDLKNFYVRSNRGTTISLDNLVTMTEEVTPPTLY
                     HFNRMKSASINCGLAPGYAVGDGIEEMERIAAQVLDPSFTTALSGTARDLAESKGNSG
                     FAFILALILIFLVLAAQFESFIDPLIIMITVPLAIAGALLSLYLFGHTLNIFSQIGMI
                     MLIGLVTKNGILIVDFANKQQLDGVNKKDSAITASVLRLRPILMTSLAMALGALPLAL
                     SLGAASTSRIPLGIVIVGGVLFSLVLTLFVIPAIYTFWSTRKHAMRAELQ"
     gene            complement(6744..7832)
                     /locus_tag="PKM27_15835"
     CDS             complement(6744..7832)
                     /locus_tag="PKM27_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR08790.1"
                     /translation="MRSNYIPTVILLLLTPFLFLRCDKKEAANGPGAAAGGAPQGIPG
                     GPGNVPVPRYDAVIARGFEISREIQSPGTILAEETTNLQPEISGRITGIYFNEGSFVP
                     NNKLLVKLNDADLLAQRSKMEVQLKIAEASELRQKELLAVNGTSQQEYDLATLAVSNI
                     KADMELNAVNLAKTEIRAPFAGKLGLRNVSLGAYVSPQTILSNISQVSTLKIEFNVIE
                     KYTTDLHIGKVVRFKTETSQNTYDAKIIAFENTLTSDTRQLKIRAQVLRPDRTLTPGS
                     FISVNFSVGSSEPAIMVPAQSIIPQARDRQVIIYQNGQANFTKVITGFRDSSRVEVLE
                     GLKAGDTILTTGLLTIRPGAKIDVRITE"
     gene            complement(8215..10101)
                     /locus_tag="PKM27_15840"
     CDS             complement(8215..10101)
                     /locus_tag="PKM27_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNR08791.1"
                     /translation="MKAFLNFAVAFSLAADLFAGPLGPFQDTTRPAHQLENPMSVQYM
                     KEKLRRNSPRLVLTPELEQELRLKIISDPVIKNVYQAIRLNTQAILREPLLERVVVGR
                     RLLATSREMLYRMNMLGMAYRMEKDPILLDRINEEVLAVCDFSDWNQSHYLDVAEMAL
                     AVAFALDWAGDQLPVSTVQAARRALIEKGLKPSYDEKGNTGWIRGTNNWNQVCHGGMI
                     AAAIVIAEEDPALASETIRRALEGIPYALDEYGPDGVYPEGATYWGYGTSFSVVTAAI
                     LESAFGTDFGLSKYPAFIEGAACQVLNTAPSGWYYNFADCGDRRNENGDMTLAWFAAK
                     TGNKTFYEKERFLMPPDKMGKLGRLDGAGLVWLAKFRDKPSAEIPQAWKGAGSNPIVI
                     FKSGPEDPHQYYFGGKGGRGLVNHGNMDGGSFVFEVNGIRWVVDPGNQDYHLLEKTGF
                     DLWGRCQNCQRWTLLTKNNFGHSTLTVNDGLHAVDGMATITGFRQGPKPEAVIDLTPA
                     FSGQLQSAQRRFVKDGPQSLLVEDLLETTKETRMITWQLMTTADIEIINGGAVLHKEG
                     KQLRVENLSHPGLTFSVISLYPAPLELDRQIPGLKRLELRIPAYLLRNEKEKIAIKLS
                     GQLP"
     gene            10654..11628
                     /locus_tag="PKM27_15845"
     CDS             10654..11628
                     /locus_tag="PKM27_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08792.1"
                     /translation="MKEQIEKILPSDDKKRKWISVGLTVIISGLLTLWGIYGIGEYGI
                     ALFILTPLFIGAGSTILYGLKKEINYKQAWQIGFLTLGVYTAGLLIFVIEGVICIAMA
                     APIGLLLTWVGSLIGYTIINKTPNNAPTTMLILIGIIPTMAFIEKDNKPTLTSVLTSI
                     EINADPQTVWKNVVEFPKLDEPTEFIFKTGIAYPINANIEGTGVGAVRHCNFTTGSFV
                     EPITVWDEPKLLKFDVVEQPDPMKELSFWDIDAPHLHDYFVSKQGQFKLTELPNGNTL
                     LEGTTWYYHNIKPTFYWQLWSDHIIHKIHERVLTHIKKNAENDKKSGI"
     gene            11845..13263
                     /locus_tag="PKM27_15850"
     CDS             11845..13263
                     /locus_tag="PKM27_15850"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNR08793.1"
                     /translation="MNKLIIFLIVVTGLTFSCNNSQTTTNEQISVTEESKQTVQTEET
                     TSDLETLKDTNKTNTTLPKQAEVIENESTISWADFYKQLEKRPQVFSINNSKDTLLSC
                     LEGTTVRIRSNTFVSILTGKPISGNIKITVTEYYKLSDILLANLTTTSDNSLLETAGM
                     IYIAAVSNNENCKIKDGQKIEIGFPTKAKNDDMQIFSGDWDNNRINWLSVSNSTDLNK
                     IYTVTDVQPQFPGGDQKMMQFISKNIKYPNIAKEFGIQGTVYIRFTIDRDGNVKDANV
                     FRGIDPDCDREALNAVLQFPKFKPAQVKGENVHITYTLPINFRLDGGITKSSDIKQNF
                     ENTYSDSTINMAHSNHITSYLFSTTQLGWINCDRFIFEKGERVDLIVKLDKKAESDVK
                     IVFHGIKSIMPSFPTNDKHTFNKIPKGEMITIVAIKNIANKPHLAIKETVTSTQTETD
                     FTFQPITMETLKSEMKKLDKIN"
     gene            13765..14655
                     /locus_tag="PKM27_15855"
     CDS             13765..14655
                     /locus_tag="PKM27_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08794.1"
                     /translation="MHKDYKRNFIRTSFYDPFKLLCFLLFYTAGCSVVLAQPPGKHWE
                     GIAYNQYRNELAVFSGAEFQDNQMFLTDTLWLFNGTWRFVDGNNITRRWAHSLAYHDN
                     LLYTYGGLSLNASHQEEVLNDLLRFDGLWSKVAEGSKLVLPTLHSVKGKLLLAGQSLE
                     DRKNFEVWELASNAFKKQSSTRLDVGNDGVRTLVVEDCFVVIYPYDSGFVFQNVTNGE
                     ISVVKDLPKRTKFGITYNHNLVSYFLFGGIDESRNLSNDLWQIREGQPEKLSTQDSPA
                     PRASCSLLPIAKGFILYGGT"
BASE COUNT         3841 a         3494 c         3586 g         3782 t
ORIGIN      
        1 acattacgtg cctaaaggca ccatttggga tgcaatttat acgcaacaat atcattacct
       61 gttttttggg ccgtagtccg aatggaggtt tctgaggagg gcggtttggg tcgcagggag
      121 gtcacagggt acgaagagta tgaatttgaa aaattcagat attttcaatt cctaccttcg
      181 tgaaccttgt gtctcagtgg gcaaccaccc ccgttaccgt tgctgcaatt aaactgtgct
      241 attcacttcg ctgtgtcgtt gtgcttttgt accgttgtgg gaaaaccgcc gtttcgtcgt
      301 gagaaattcg aaacatatat acctccagca tcgaagatgc tccacgtttt taatactcat
      361 tcaagtacaa ccccggtgct tcggagaagc ccaacatcgt cgaacatcta cgatacaagg
      421 cctcaccgaa cgcccaccaa aaagatggcc actccaaccg cctatatcga aaaaaataat
      481 gtctttttaa ttgggactac gattgttgag cttctccgaa gcccttgttt tgttcttatt
      541 ttttaccaaa aacgtcatgc atctccgatg ccggaagaaa ccgggggaat caaatccttg
      601 ctcataaatg cacctagcct tgcatgaact ctttaatgct ctttgagacg ttgtgggaac
      661 cactgcctgt aggggccaaa gcccgaatgg gagcggccga agcaaagcgt cgtccgaacg
      721 ggtgctcttc gcttcgccgt tgtgggaacc atcgtcgtgt cgttgtggga accattgtcg
      781 tgtcgttgtg ggaaaaactt ttgtgccctt cgcttcgctg tgtcttagta ggcatcccat
      841 acggcccttg tgggaaacct ctcaagcatg tcgtgtcgtt gtgggaatta aatagtgtcc
      901 ttcgcttcgc agtgcctttg tgggcaacct tgcagttcac ccaactgctg cacgcttcgt
      961 gggcagcccc tttggtcagc ctccatccat accacgacat ggtggtctga tagcggctta
     1021 tcaggacttg gaagaagcct tggccacggg acggtagccc ggggtcttat cggaaagggt
     1081 cctgagcacg gaacggagct tttccgtcaa ctgatccacc tggttggcct ggatgctcag
     1141 ggggggcaac aaacggagcg tattgggatc ggcggcggat ccggtaaaca ccctttcttt
     1201 gaataccagt tgtttgcgga ggtcggcgac cggaaaatca aattccacac ctaccatcag
     1261 gcctttcccg cgcacttccc tgacctgggg aatttgggtc atttcagcca tgaggtattc
     1321 gcccacctcg gcggagtggc tgaccaaatc gtcctcttcc atcacttcga gtacggcgag
     1381 agccgcggca caggccaggt gattgccccc gaaagtagtg cccagcatgc catgcctggc
     1441 tttgatcccg ggctggatca ggacgccgcc gatgggaaat ccattgccca tgcctttggc
     1501 gaccgtgatc aggtcgggcc ggatatcgtc cgcatgctgg taagcgaaaa acttgccgct
     1561 ccggccatac ccgctttgaa tttcatccag tataaaaagg gtttccgtct tccggcaaag
     1621 cgtttgcagt tcccgtagga aggtcgcatc cggaacatag accccgccga tgccctggat
     1681 gccctcgatg atcacgcagg catactcccg gctttccagc aattcttcca gccggccggt
     1741 ttcgttgagc ggacagaatt cgacgtcaaa ggttttattg atttccgact tgattttcgg
     1801 gtcatcggtg acgttgcagg cggcggaggt acggccatgg aagctttttt taaaggagat
     1861 cacctttttc cggccattct ggaaagaagc cagtttgatg gcattttcat tggcttccgc
     1921 gccgctgttg atcagaaaaa gctggtagtc ttcgcagccg ctgattttac ccaatttatc
     1981 cgccagctct ttctgcaggg aattgatgat gctgttggaa taaaatccaa gtttttgcag
     2041 ttgtttagtg atccgctgga tataatgcgg gtgggaatgg ccgatggaga tcacggcatg
     2101 tcccccgtaa aaatccaggt actccacccc tttctgatcg tacacataac agcccctgcc
     2161 ccggaccggc tcgatatcgt acaggggata aacatcaaaa agattcatac ggcaaattta
     2221 tgaagatttg aaaaggaggg gtaaaatgct ttgaacatca ttgttccacc aaccggccca
     2281 ttacatagag gatctgggct tcgctctgcc ggaggataaa ccggctgttg atgatccggt
     2341 tctgggcttc tatgaggttg agctgggcct gcctgagttc cacggtggtg atgctggcct
     2401 tgcggaagcg ctccatggag atggcattgt gttcgcggat gatcgccagg ttttgttctt
     2461 ccagcccgat ctggcggttg gcgttttcat aattctggta ggcgttggca gccaagctgg
     2521 tcaggtcgtt ttttacattc tggatctgga ggtcctggtt tttatattgc agatcattga
     2581 ccttgacctg ttggcggacg atggggcctg caaaaagggg cacggaaagg ctcagcccgg
     2641 cctgggggcc ataggtcatg gtaaagaggt taaaaccggc ggtgctgctg ttgcggttga
     2701 aattggcgga tgcgttggcg aaaacgctgg gtttttgggt ggccaggatt tcctcccgga
     2761 gctgggtaag aattttttga ttaaagcgca gggcggtgat ctgggggttc tgggcatcga
     2821 tggcctgcat catttcctcg cgggagggca ggatttccgg cgcggggata tcttccacct
     2881 caaaggcggt gaaggggtcg cggaccatga cccggttgag ttccattttg gccagttcga
     2941 tgctgttgag gatattcagt tcgctggtcc gctgcacgtt gaggtccacc tgggattgca
     3001 gataatcatt cttgccggcg gttccgatct cgaagcggac ctgggaaagt ttgcttcgtt
     3061 cttcaaaaaa ggaaatggtt tccctgacgg catccagctg gctcttgagc cggacaatat
     3121 cgatataccg gttgatgaca tcaaagaccg tctggttgat gtcctgtttc agcacttcgt
     3181 cggccagggc ttcttcgagg tccaggcggt ttttgatgat atacatccgt ttccccccgt
     3241 agatccggta actgaactgg ccgttgacgt tgatcgtgct gttcctcgcc ccgttgcgtt
     3301 caatgacggt accgttgttc agtttctggt tgaggttcgt gatggaattg ttgaacgtgc
     3361 tgttcaaacc cacggtgggg atccgtccgg cggcccacca attgttgttt acctgggcga
     3421 tctctttttc gttggaggag atcaggatat tcggattctg gcttaaagcg atttctatgg
     3481 cgtcatccag gcgaaggaca gtctgagcca tggtcacaga agtgaataaa acaaagaaca
     3541 aagtgagttt catattctgg gtcaggatat gccttgaaaa ggttttggat atgaatattt
     3601 acgtaccatg gccaatgact tattgcagtt cggcccgcat ggcgtgttta cgggtactcc
     3661 agaaggtata aatggccgga atgacaaaga gggtcaggac cagggaaaac aagaccccgc
     3721 ccacaatcac aatgccaagc ggtatgcgcg aagtggaagc cgctcccagg gaaagggcca
     3781 ggggaagggc gccgagcgcc atggccaggc tggtcatgag gattggcctc agcctgagca
     3841 cggaagccgt gatggccgaa tcttttttat tcaccccatc cagttgttgt ttattggcaa
     3901 aatccacgat caggatcccg tttttcgtca ccaggccgat caacatgatc atcccgatct
     3961 gggaaaagat attcagggta tgtccgaata aataaaggct cagcagggcg ccggcgatcg
     4021 ccaggggcac ggtgatcatg atgatcaggg gatcgataaa actttcaaac tgggctgcca
     4081 ataccaggaa gatcaggatc aaggccagga tgaaggcaaa accgctgttt cccttgctct
     4141 cagccaggtc acgggccgta ccggacaggg cggtggtaaa actcgggtcc aggacctggg
     4201 cggcgatcct ttccatttcc tcaatgccat ccccgaccgc atatcctgga gctaatccac
     4261 aattgatgct ggccgatttc atccggttga agtgataaag ggttgggggt gtgacttctt
     4321 cggtcatggt gaccaggttg tccaaggata tggtcgtacc ccggttgctg cgcacataaa
     4381 agtttttgag gtcgttagga tcgtcgcggt cgttgcgcat cacctgtccg atgacctggt
     4441 attgtttgcc gtccttgata aaataaccca gcctgcggtt gctgagggca agctggaggg
     4501 cttcggacaa gtccccgatg ctcacgccca gttcactggc tttgaggcgg tcaatctgga
     4561 tgcgcagttc aggtttgttg aatttgagat caacgtccgc gttcaccagg atttggcttt
     4621 tttgaatttc ttccagaaac cggggaaggg cttccttgag tttctcaaaa ttattgtttt
     4681 gcactacaaa ctgcacgggc tgcccgccaa agcgacggcc aacctggatt gtttggtcct
     4741 gggcggcgaa catccgcaca tcggtaaatt ggggaaggcc tttactgact gcctggacga
     4801 tctgggcctg tgatctctcg cgatcatggg gatcggagag gacgacgcga acgaatccgg
     4861 tattgaccga accgctgccg gtaaacccgg gggcggtcat gctcatcacc gttgtttttt
     4921 caggcacctg ttccaggaca taatcggtga tggcgacgat gttgcggtcc atcgcttcat
     4981 aagaggtccc ctcgggggcg gtcacctgga tcctgaatac gctgcggtct tccatcggcg
     5041 ccagttcgga cttgattttg ctgccgataa aatagatgac gcccccgcag gctaaaacga
     5101 tcacaaaggc catccacctg actttgagga aggcgctcag gctgcggcgg tatccgtttt
     5161 ccatgccctt gaaaaaaggc tccgttacac ggtaaagcca ggaatggtgc gcggaggtag
     5221 ccattttgac gttcaatacg ggagtgaggg taagggatac cagggcggag atcaataccg
     5281 cgccggcgac cacaatgccg aactccctga acagccgtcc cacaaaaccc tggaggaaaa
     5341 tgatggggat aaataccacg gccagggtaa tggaagtggc cagtacggcg aaaaagatct
     5401 ctttggaacc ttcccgcgcc gcctgccatt tgttcatgcc cgcttcaatt tttttataaa
     5461 tgttctccgt gaccacgatg ccgtcatcca ccaccaggcc ggtggccagt acaatggcga
     5521 gcagggtgag cacattgatg gtaaagccaa agaggtacat gatgaaaaat gcgccgatca
     5581 gggaaaccgg gatgtccagc aaaggcctca gggcaatgcc ccaactccgg aagaaaaaga
     5641 agatgatcgc gatcaccagg ctcagggaaa ggatcagggt ttcctttacc tcatccaggg
     5701 atttccggat aaacttggtg ctgtccaggg cgatcccgag tttgacgtct tccggcacat
     5761 ccctttttaa ttgatccacc ctccggtaaa attcatctga gatgttcaca tagttggtac
     5821 ctggctgggg aatgatggcc agggagatca tggggatatt gctttcccgg aaaacggatt
     5881 cttcattctc cggtcccagg accgcttcgg ctacatctcc cagcctgaga atccccgtct
     5941 cactgctccg gatgatgacg ttgttgaatt cttcctccgt attcaaccgg ccgaaagtcc
     6001 gcacactcaa ttcgaccgcc tcaccggata ccttgccgct cgggatttca acactttccc
     6061 gctggagggc cacctggatg tccttggcgg tgatcccgaa tgcattcatt ttatccggat
     6121 ccatccagat cctcatggca tatctttttt ccccccagat ctggatgctg ctcacgccgg
     6181 gaatggtctg cagcctttcc acgaggatat tattcgcaaa ttcggtgatc tccagctgat
     6241 tccggttggg gctttgtacg gtcatgctga gaatgggatc gctgcttgca tcggccttgg
     6301 atactacggg aggcgcatcc aggtctgccg gaaggctgcg tgccgcctgg gacactttat
     6361 cccggacatc gttggccgct tcttcgaggt ccaccccaag ttcgaattcc acattgatat
     6421 tgcaattgcc ctgggaactg ctggacgtca cattccggat gcccggcacg ctgttgatgg
     6481 ctttttccag cggttcggtg atttgagatt cgatgatgtc cgcattggcg cccgggtagg
     6541 tgcaccggac gttgatgaac ggcggatcga tcgccggata ttccctgacc ccgaggaatt
     6601 taaaaccaat gattccaaac aaaaggatga tgaggttgag cacgatggca aacaccggcc
     6661 ttctcagact gacttccgat atattcattg gtttggatta aaggttagaa aataaaaggt
     6721 catatgcagg gtcgcttctg gtcttattct gtgattctta catcgatttt cgcgccggga
     6781 cggatggtta gcaaaccggt ggtcagtatg gtatcgccag cctttaatcc ttccaggacc
     6841 tctaccctgg aagaatcccg gaagccggtg atcactttgg taaaattggc ttgcccattc
     6901 tggtatatga ttacctgccg gtccctggcc tgcggaatga tcgattgagc cggtaccatg
     6961 attgcaggtt ccgaggaccc tacactaaaa ttaaccgaaa taaaacttcc gggagtaagg
     7021 gtacggtccg gccggagcac ctgggccctg atcttcaact ggcgggtatc gctcgtgagg
     7081 gtattttcga aggcaatgat tttggcatcg taggtattct gggacgtttc cgttttaaac
     7141 cgcacgacct tgccgatatg aaggtccgtg gtatattttt cgatgacatt aaattcgatc
     7201 ttgagggtgc tcacctggga gatgttggaa agtatggttt gggggctgac ataggccccg
     7261 aggcttacat ttctgagtcc cagcttgccg gcaaaaggcg cccggatttc cgttttcgcg
     7321 aggtttacgg cattcagctc catgtccgcc ttgatattgc tcaccgccag ggtggcgagg
     7381 tcgtattcct gctggctggt cccgttgacg gccagcaatt ctttctggcg caattcactg
     7441 gcttcagcaa tcttgagctg gacttccatt ttcgaacgct gggccagcag atcggcatca
     7501 ttcagtttaa ccagcaattt attattggga acaaaacttc cttcattaaa atagatgccc
     7561 gtgatccttc cggaaatttc aggctgcagg ttggtggttt cttccgccag gatggttccg
     7621 ggggactgaa tttccctgct gatttcaaac cccctggcaa tcaccgcatc gtacctggga
     7681 acgggaacat tgcccggtcc ccccggtatt ccctgggggg ccccgccggc cgcagctcct
     7741 ggtccgttgg cagcctcttt tttgtcgcag cgtaaaaaca ggaaaggggt caataacagc
     7801 aaaatgaccg ttggtatata atttgatctc atgaattttg caaaattaag gtctctgcgg
     7861 acacttttct gtcctgtggg gagactttcc ggattattag acggacaggc ggtttaatta
     7921 taccgatcat tccaaggcac ttaaaaaaaa aagttggctt tccggggtga catttcatac
     7981 tgttaataca ctaataagaa aatgttatca aaccaacttt cactgccaaa gcaaatggcc
     8041 tggatcgccg cgcgcgttcc gggaatgacc atggggaacc ttgcagacca catcccttat
     8101 cctggaatcc ctatccggca tctggccggg aggattacca gggagcggac tttggcacac
     8161 cttaaatctg acgattcaat tttgaaatgc tgaaccggtc aattccgtat aaagttatgg
     8221 caattggccg gacagcttta tagctatttt ttccttttca ttccgcaaca gataggccgg
     8281 aatccttaat tccagccgtt tgaggccggg gatctggcgg tccaattcga gcggcgcggg
     8341 atataaggaa atgaccgaaa aggtcagccc gggatgcgaa agattttcca ccctgagctg
     8401 tttgccctcc ttatgaagga cggcgccccc attaatgatt tcgatatcgg ccgtggtcat
     8461 cagttgccag gtgatcatcc gggtttcctt tgtggtttcc aggagatctt cgacaagcag
     8521 ggactgggga ccgtctttga caaatcttct ttgtgccgat tggagttgac cggaaaaagc
     8581 cggagtcagg tcgatgaccg cttcaggctt ggggccctgc cggaaaccgg tgatggtggc
     8641 cattccatcc acggcatgca atccatcatt caccgtgagg gtgctgtgcc caaaattgtt
     8701 tttggtgagc aaagtccatc gttggcagtt ttggcatctt ccccagagat caaagccggt
     8761 cttttcgaga aggtggtaat cctggttgcc cggatccacc acccacctga tcccattcac
     8821 ttcaaaaaca aaggatcccc catccatatt tccatgattt accaggcccc ttcccccctt
     8881 gcctccaaaa taatattgat gcggatcttc aggcccgctc ttaaaaatca ctatgggatt
     8941 ggatccggcc cctttccagg cttggggaat ttccgcggaa ggcttatccc tgaatttagc
     9001 caaccatacc agtccggcac catccagacg gccgagttta cccattttat caggaggcat
     9061 taaaaacctt tccttttcat aaaaagtttt attccccgtt tttgccgcaa accaggccag
     9121 ggtcatatcc ccgttttcgt ttctccggtc accacaatcg gcaaaattgt agtaccagcc
     9181 ggaaggcgcg gtgttgagta cctggcaggc ggcgccttca ataaaagctg ggtattttga
     9241 caatccaaaa tccgtcccaa aggcgctttc caagatcgcc gcggtgacta cactgaagct
     9301 ggtgccgtat ccccagtaag ttgctccttc cggatatacc ccgtccggcc cgtattcatc
     9361 gagcgcgtag ggtatgcctt ccagggcgcg gcggatggtt tcggaagcca gggcagggtc
     9421 ttcttccgca atcacgattg cggccgcgat catgccccca tgacaaactt ggttccagtt
     9481 gttggtgccc cggatccagc cggtattgcc tttctcgtca taactgggtt tgagaccctt
     9541 ttcgatcaga gcccggcggg cggcctggac cgtcgatacg ggcaactggt cccccgccca
     9601 gtcaagtgca aaagcaacgg ccaatgccat ttccgccacg tcaaggtaat gggattgatt
     9661 ccagtcgcta aaatcacaga ccgccaggac ttcctcattg atccggtcca gcaggatggg
     9721 gtccttttcc atccgatagg ccatacccag catattcatc cggtacagca tctcccggga
     9781 agtagccaaa agccggcgcc cgacgaccac tctttccagc aaaggttccc tgaggatagc
     9841 ctgggtattt agcctgatgg cctggtaaac atttttgata accggatcgc tgattatttt
     9901 gagcctgagc tcctgttcca actcgggagt cagtacaagc ctggggctgt tgcggcgcaa
     9961 tttttctttc atgtattgaa cggacatcgg attctccagc tgatgagcag gccgggtggt
    10021 atcttgaaaa ggacccaaag gacccgcaaa caaatctgca gccagggaaa aggccactgc
    10081 aaaattcaaa aatgctttca tacctttttt ttggatcctt taaaataaag aacagaatgg
    10141 aaaaaatcac atgaaacaaa gcttctcgct tcacgcttct cgcctctcgc ctctcgcttc
    10201 tcgtttctcg cactcgtttc tcgcttctcg tttctcgctt ctcggtctgt ttccagctac
    10261 aaacagctat atccggctac attcgaccac aaacggttaa tatccgccta caaacgatta
    10321 attaggccta tataaaaaat cccagttaat ttcttgaata ttatcggttt aaattatatt
    10381 ttagcaatgc cggcaaattg aacacccggt gaatcaggct gcctttagac attttgctaa
    10441 caaataaacc actatgaaga tccacgctac ttttcacacc ctcctgaact cttacgcctt
    10501 acatacattg aaagcataag tgcatctcag gcattcagtc tgtctcggtc cataatacat
    10561 tgaaaaccat atgaatggat taaatgatac aggtagattt acgaaatatt gcgtaaacac
    10621 aaatgttata tgccacttta ggacgacacg aaaatgaaag aacaaataga aaaaatattg
    10681 ccaagtgacg acaaaaaacg aaaatggatt tcggtcggac tgactgttat catttcaggt
    10741 cttttgacac tttggggcat ttacggaatt ggagaatatg gaattgcctt gttcattttg
    10801 acacctcttt tcattggtgc gggttcgaca attttatacg gactaaaaaa ggaaattaat
    10861 tacaaacagg cttggcaaat cggctttttg acacttggag tttatacagc aggactttta
    10921 atttttgtaa ttgaaggagt aatttgtatt gcaatggctg caccaatcgg acttctattg
    10981 acttgggttg gtagtttaat tggttataca ataatcaaca agacaccaaa caacgcacca
    11041 acgacaatgc taattttaat cggtatcatt ccgacaatgg cgtttataga aaaagataac
    11101 aaaccgacct tgacttcagt attaacttca atagaaatta acgcagaccc acagacagtt
    11161 tggaaaaacg tagttgagtt tccgaaactt gacgagccta ccgaatttat ttttaagaca
    11221 ggaattgcct atccaataaa cgcaaatatt gaaggaacag gagttggtgc agttagacat
    11281 tgcaacttca cgacaggaag ttttgttgaa ccaataaccg tatgggacga accaaaactt
    11341 ttaaaatttg acgttgtaga acaacccgac ccaatgaaag aattaagttt ttgggatatt
    11401 gacgcaccac atttacacga ctatttcgtt tctaaacaag gacaattcaa attgacagaa
    11461 ctaccaaacg gaaacacact tttagaaggc acaacttggt attatcacaa cataaaaccg
    11521 acattttatt ggcaactttg gagtgaccat atcattcaca aaatacacga acgagttttg
    11581 acacacataa agaaaaacgc tgagaatgat aagaaaagcg gcatataaca cgggttttgg
    11641 gtcaggcggg gtgacgtgca aaattggggc tttcccgcaa aaacgggaca agttatgctt
    11701 ctattcaagt ttagtgtagg ttggcagttt tgtgctcccg atgcttcgat agaaacaggt
    11761 ccgaaacccg cctgaacgca aagcccgaaa ccgttagcag caatattgac tgaccagaca
    11821 cgttaaataa aaaacgttat gacaatgaat aaactgataa tatttttaat cgttgtaacg
    11881 ggactaacat tttcctgcaa caattcgcag acaacaacca acgaacagat ttctgtgacc
    11941 gaagaaagca aacaaactgt tcaaacagag gagacaactt cagacttaga aaccttaaaa
    12001 gacactaaca aaacgaacac tactcttccc aaacaagcag aagttataga aaacgaatcc
    12061 acaatttctt gggctgactt ttataagcaa cttgaaaaaa ggccgcaagt attttctata
    12121 aataatagca aggacacttt attgtcttgc ttggaaggga caacggtaag aattcgttct
    12181 aacacattcg tttctatatt gacaggaaag ccaatttcag gaaacataaa aattactgta
    12241 acagagtatt acaaattgtc tgacatacta ttagcaaatc tcacaactac atccgacaat
    12301 tcacttttgg aaactgctgg tatgatatat attgccgcag tttcaaataa cgaaaactgt
    12361 aaaataaaag acggacaaaa aatagagatt ggttttccaa caaaagctaa aaatgatgat
    12421 atgcaaattt tcagcggaga ttgggacaac aatagaatta attggctatc cgtttctaac
    12481 tcgacagact taaataaaat ttatacagta acagatgttc aacctcaatt tcccggagga
    12541 gaccaaaaaa tgatgcaatt catcagtaaa aacattaagt atccaaacat agcaaaagaa
    12601 tttggcatac aaggaacggt ttatattcga tttacgattg acagggatgg caatgttaag
    12661 gacgctaatg ttttcagagg catagacccc gattgcgaca gagaagcatt aaatgcagtg
    12721 ttacaatttc caaaattcaa acctgcacaa gttaaaggag aaaatgtaca tattacgtat
    12781 acattaccta tcaatttcag acttgacgga gggataacga aaagctcaga cattaaacaa
    12841 aattttgaaa acacttatag cgactcgaca atcaacatgg cacattcaaa tcacattaca
    12901 tcctatttat ttagtacaac acaactcggt tggattaact gtgacaggtt tatttttgag
    12961 aaaggagagc gagttgacct catagttaaa cttgacaaga aagctgaaag tgatgtgaaa
    13021 attgtatttc atgggataaa atctataatg ccatcattcc caacaaatga caaacacact
    13081 ttcaataaaa ttccaaaagg agaaatgatt acaattgtgg ctattaaaaa tattgccaac
    13141 aaaccacatc ttgcaattaa agaaactgta acatcgacac agacagaaac cgactttact
    13201 tttcagccaa ttacaatgga gactttaaag agtgaaatga aaaaactaga caaaattaat
    13261 taatagaatg acaagaaata ccgcagctaa cactggttgt gcgtcaggtg ggctgacccc
    13321 gtttcgtgcc tcacggggca gacagtgcaa actcggagct atcccgcaaa aatcctacaa
    13381 gttgtgcttc tattcaagtt cagtgcaggg tgacagtttt aatgatgaat cgggacaggt
    13441 cgggctcccc tgcctgcgtg acgcagtcag gcagggaaat ccgccactgc gccaagcgcc
    13501 aaaacgtcag cgcccagtgc cgggaaatgc aacgtaattt taattgttcg atgtggattg
    13561 gttccagccg gcatgagtgt atccacatat caacaaaaga gagaaaaagg atcaaaaaga
    13621 aagaaaacca atacaataac aacaaaatca ataattttaa agaatgatca acaaaggaat
    13681 acactttaat attccgaaaa aactgtctca acaattgggg aactaaatat tatgtgcact
    13741 ttggtttttt aataaacggc atcaatgcat aaggattata aacgcaactt tattagaacc
    13801 tctttttatg atccgttcaa attgctatgc tttctattat tttataccgc gggttgcagt
    13861 gtggttttag ctcagccacc gggaaaacat tgggaaggga tcgcctacaa ccagtataga
    13921 aatgagctgg cagtctttag tggtgctgaa ttccaggaca accaaatgtt ccttactgat
    13981 acactttggt tattcaacgg tacatggcgc tttgttgacg gcaacaatat tacccgccga
    14041 tgggcacaca gtttagctta tcatgataac ttgttgtata catatggtgg attgagttta
    14101 aatgcgtccc accaggaaga ggtgctcaat gatttacttc gatttgatgg attgtggagc
    14161 aaagtcgcgg aaggctcgaa acttgtttta ccaacacttc attcggtaaa gggaaaattg
    14221 ttgttggctg gtcagtcgct tgaggatagg aaaaactttg aagtttggga acttgccagt
    14281 aacgccttca aaaagcaaag ctcaaccagg ctggatgtgg gaaacgacgg agttcgaaca
    14341 ctggttgttg aagattgttt cgtggtgatc tatccctacg actccggatt tgtttttcaa
    14401 aatgtaacaa atggggaaat ctctgttgtg aaagatttac ccaaacgcac aaagtttggc
    14461 atcacttaca accataacct tgtaagctac tttttattcg gaggaataga tgaaagcagg
    14521 aatcttagta acgacttgtg gcaaatcaga gagggacagc ccgaaaaatt gagcacccaa
    14581 gactcccctg caccgagggc ttcctgtagc ctcttgccaa tagccaaagg cttcatatta
    14641 tacggcggta cgtaagcacc cggccaacca catattgatc aaggcaagag tggggcatag
    14701 att
//
LOCUS       DAOQXT010000109        14503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4630_length_14503_cov_5.764673, whole genome shotgun sequence.
ACCESSION   DAOQXT010000109 DAOQXT010000000
VERSION     DAOQXT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14503
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4630_length_14503_cov_5.764673"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PKM27_15860"
     CDS             <1..814
                     /locus_tag="PKM27_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HNR08795.1"
                     /translation="HHPDLGLDYDWNLFRELKRMYPRLKLHALGPPEIAHISKISGTT
                     HLETLTALKEAGLDSFPGAGAEILVDRVRNLIARGKCLSQEWLDVMRAAHQLNLTTSA
                     TMMFGTVETIEERMEHLVRIREVQAEKPEGATGFLAFIPWPFQDEGTLLQRLKRAENN
                     TTAEEYIRMIALSRLMLPNITNIQASWLTVGKSVAQICLHAGANDMGSIMIEENVVSA
                     AGAPHRFTAKGIQQAIREAGFEPQLRNQHYQKREMPENMEEQRIEPVGLRVN"
     gene            complement(934..2301)
                     /gene="radA"
                     /locus_tag="PKM27_15865"
     CDS             complement(934..2301)
                     /gene="radA"
                     /locus_tag="PKM27_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNR08796.1"
                     /translation="MKLKKVYYCSNCAAKSPKWIGHCPSCDQWNTYVEEIVDVSKPGD
                     ALQKSLRSQKPGAKPVLLSEIQKSAAKRLITPDHELNRALGGGIVQGSLILIGGQPGI
                     GKSTLMMQLALNLKAKILYVSGEESEEQIKMRADRMGLTPNHCYILTETELSRVLDEI
                     QALQPDLVIIDSIQTLTLPTVESIQGSITQVRECTAEIQRYAKQSGIPFILIGHINKE
                     GMIAGPKVLEHIVDVVLQFEGDTFHAYRILRTRKNRFGSTDEMGLYEMQANGLRQIHN
                     PSELLLSQTQENLSGSAVCATVEGQRPVLLEVQALVSTALYGTAQRSATTFDLRRLSM
                     LLAVLEKRVGLRFSSYDVFLNIAGGLKINDPALDLAVVAALVSSLQDMAIDKHIAFAG
                     EVGLSGEIRAVSRVEQRIQEAERLGFNRIYISSNNLKGLSKTKNKIQVTALGKVNDLL
                     SDLFG"
     gene            complement(2576..3334)
                     /locus_tag="PKM27_15870"
     CDS             complement(2576..3334)
                     /locus_tag="PKM27_15870"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNR08797.1"
                     /translation="MNLLVTDVGNSDTVFGIWHSGDESPLVYRLETASLINDPSLLSQ
                     FLQKGFGGFRPPERVVVSSVVPAATQQVTKALAQWSKQDIKVLEKSQFEKLPLVILRP
                     DEIGTDLVANALAAHNLSKGASIVVDFGTALSFTTIDATGTILGVSIAPGLRTALQAL
                     TTHTAQLRTVPLQMPESALGKSTEQAIQTGILYGYDGLVRGIVSAQEKELGLDLFLIA
                     TGGLSKMIHTLEDRVNLYAPHLTLEGLKIAAQYL"
     gene            3398..4015
                     /gene="scpB"
                     /locus_tag="PKM27_15875"
     CDS             3398..4015
                     /gene="scpB"
                     /locus_tag="PKM27_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNR08798.1"
                     /translation="MELLELQIESLIFISSEPLKPEDLIACLEAYHKEKIEPTLVMEC
                     LEKIKTKYASDDFAFELNEIAEGFQFLTKAAYHPIVRTFLNQMSQKRLSRTALETLAI
                     IAYKQPVSKPEVERIRGVNCDHTIQKLLEKELITMEGRAEGPGKPILYVTSEKFMNYF
                     GLKSIDQLPNIKEFNHGFNEVGEEIPIERMVMDLEQDISANKNNE"
     gene            4012..4533
                     /locus_tag="PKM27_15880"
     CDS             4012..4533
                     /locus_tag="PKM27_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HNR08799.1"
                     /translation="MNTESTLVNRVAESELQTIALDDMIRDAAVVSFDLKDYLFQGLI
                     LKEKDFRESLKATDWSKYRDQILAVHCSTDAIIPMWAYMLIAAYAAPVAKAIYYGGKQ
                     EVLEQVLLERINQKDWEYLRGKRVVIKGCSDQELPASAYLQIVRQLQPLAQSIMYGEP
                     CSTVPIFKRPRVV"
     gene            4703..5620
                     /locus_tag="PKM27_15885"
     CDS             4703..5620
                     /locus_tag="PKM27_15885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNR08800.1"
                     /translation="MNKLMHVYSFVLAGLLSGLLFTSYVKPEKGGASGTGEDKETKIP
                     QAIHAVPIREKYSFGGENVPIQNFDVRERLDRELLVNTYWHSNSILCIKAAQRYFPVI
                     EEVLAKYRIPDDFKYIPVIETSLRNETSPAGAKGIWQFMKSVAESYDLEVNEEVDERY
                     HFEKATEAACRHILRYKQRFGSWTLAAAAYNAGEGNLAKELGRQGERSYYDLNINDES
                     SRYIFRLLAMKDIMEHPDDYGFFIQPEEKYSRNNTKKIEVKTAVPNLGDWARENGTTY
                     RMLKLMNPWLRTSSLTNKLKKTYYIEVPK"
     gene            complement(5622..7649)
                     /locus_tag="PKM27_15890"
     CDS             complement(5622..7649)
                     /locus_tag="PKM27_15890"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNR08801.1"
                     /translation="MVLLLTLLWSFLQIGIGDSKPVEWSHRIEQLDSSRYRLIFELKI
                     IKGWGTYSQYQESAETSVKPIEFHFEDSSALRFLGKNEESGHIKKSFDKVFEEEVVKI
                     TEEAQFVQVVEILRPGEAITGYVDGIVCDDVQCIPFDYDFSFTVPGLAQPAEKTAQEG
                     SPEKKLLDQSVSSLQSSYQTVTSDCAVEEKKIDVSLLLTFIFGFLGGLLALLTPCVFP
                     LLPLTVSFFTKSSHNRATGIRNGVLYGLAIIVIYVGLGLLITSFFGETALNDLSTNWL
                     ANLLFFIIFVVFAFSFFGYYEITLPSSWSTKTDALGRSGNLLGIFFMAFTLAIVSFSC
                     TGPIIGTALVSSATSKVGPAVVMFGFSLALALPFGLFAAFPAWLQSLPRSGSWMNSVK
                     VVLGFLELALALKFLSVSDMTSHWGILKYELFMGLWILIFAAMTAYLMGWFQFPHDSP
                     MKKRSTLRTAFAALSAVWTLYLATGFLFNEKTKTYQSLSLLSGLAPPAYYNYFSPLPE
                     ADPDLKARFSSFSKCANNLDCFKDYQEGLVYAKEVQTPILLDFTGYGCVNCRKTEEHI
                     WVEDEIWSRLAGDFVLVSLYCDDETPLDEVIYSKSRDKKIRNIGNKWEDFQIVNFQQN
                     SQPLYVPVDPNDQEVLIPARGYRSSVKGYQQFLDCAYEAFKSKQSGLLGRR"
     gene            complement(7655..8095)
                     /locus_tag="PKM27_15895"
     CDS             complement(7655..8095)
                     /locus_tag="PKM27_15895"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNR08802.1"
                     /translation="MTVLILLSLFFNSGKVPVSWSHSVEKTGAQEYKIIFTAAIEEGW
                     NIYSQYLASDDGPVRTSFTFDAGPHFALVGKNEESGTIKKGHDEMFDMEVIKISKKGT
                     FTQKVKVLDPSKPITGIIEYMCCNELQCLPPKEVPFTIDLTSVK"
     gene            complement(8157..8879)
                     /gene="purQ"
                     /locus_tag="PKM27_15900"
     CDS             complement(8157..8879)
                     /gene="purQ"
                     /locus_tag="PKM27_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNR08803.1"
                     /translation="MHDLKFGVVVFPGSNCDDDLVHVVGEVCKVHTLKIWHKDHKLPD
                     LDGILLPGGFSYGDYLRSGAIARYANIMPAIKDFADQGGFVWGICNGFQILCEAGMLP
                     GVLLANRDQKFISDNVHLRVQTSNSMITSALPKGTVLRMPIAHADGRYYADQEVLDQL
                     IQNDQILFKYCDAEGNPSEAANVNGSLLNIAGICNIHRNVYGMMPHPERASEAMLGNE
                     DGLGLFRSMIHNLVAANPVAGS"
     gene            complement(8881..9837)
                     /locus_tag="PKM27_15905"
     CDS             complement(8881..9837)
                     /locus_tag="PKM27_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08804.1"
                     /translation="MLSLFRKNGFINSLLLLAYTFLLQIIPAALGQGRIPPSDIVRSP
                     FLASLLHILMLFIQAIFINRMVIENRLHRDIMLYPGVFFILYSSILPEFWSFSHLHLA
                     NFMVIWALHELFQIYKTSSPAVEIFNASFLIGIASIFCPPTILYLFLIFIGVNNLKKM
                     ELVHPIQILIGGALPWFLWFTWKFWRGHGLEVFPSFGNHMGLNVLEIGSTTADMLKGG
                     FFLFLLIFSILLYNEFRKKKHIQAQKKMDILYFALFHSVLVGLFHQPFGWNVLLLGAP
                     MLGIFAGLAISHLKNQTLAETIHFILFLGVILFQLFYFVTLA"
     gene            9987..10961
                     /locus_tag="PKM27_15910"
     CDS             9987..10961
                     /locus_tag="PKM27_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNR08805.1"
                     /translation="MEKDLLAIQRPIEKELGLFEVYFRNAMKSQVSLLDRITYYIVKR
                     KGKQIRPMFVFFSAKLVDEIQPATYTAAALVEIIHTATLVHDDVVDDSMQRRGFFSIN
                     ALWKNKIAVLVGDYLFAKGLDISIHEKQYEILAILNNAIKELSEGELLQIEKARKLDI
                     NEQVYYEIIRQKTASLIAASCAAGVASVTKDPGIIARVKQIGEWIGMAFQIKDDLFDY
                     GEASIGKPTGIDIKEKKMTLPLIYTLQHAGGGLKREIMNTMRHHSEDRRKVKEVIEAV
                     RSNGGLEYSTRIMHEYADKAKKELLTFPDSTARASLASLIDFTINRKS"
     gene            complement(10956..12266)
                     /gene="murA"
                     /locus_tag="PKM27_15915"
     CDS             complement(10956..12266)
                     /gene="murA"
                     /locus_tag="PKM27_15915"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNR08806.1"
                     /translation="MITDAFEVQGNCQLKGTLVPQGAKNEALQILCAVLLTREKVTVS
                     NIPGILDVTRLIELLGGLGVKVEKLAEGTYAFTAADLDREYMASDEFDKKAKAIRGSV
                     MLIGPLLARLGKMYLPKPGGDKIGRRRLDTHFEGLTQLGAKVEYDPAKAQYFIEAKNL
                     NGAYIHMEEISVTGTANVVMAAVLAQGTTTVYNAACEPYVQQLCRMLVRMGAHIEGIG
                     SNLLTITGVPVLKGTEHRMLPDMIEVGSFIGLAAMTRSEITIQQAGIQNLGIIPNVFR
                     KMGVTLIEKGDDLYIPALDEYEISTFMDGSILTVYDSPWPGFTPDLMSVILVMAIQSK
                     GSVLIHQKMFESRLFFVDKLIEMGAQIILCDPHRATVIGLNRKHFLRGIEMSSPDIRA
                     GVALLLAALSAKGTSLIHNVNQIDRGYQNIDGRLNAIGARIRRL"
     gene            complement(12399..13091)
                     /locus_tag="PKM27_15920"
     CDS             complement(12399..13091)
                     /locus_tag="PKM27_15920"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNR08807.1"
                     /translation="MNYNSTRSLLLYPEYGRNVQEMIRSIRNEEDPRKRQAMANAIVS
                     LMGHLAPGPKNGSDIRPKLWKHFFAIAEYQIDVVSFEGIRYEAEESNIKPVPVDYPPT
                     CETYRHYGNHVRRLIEKASQMEDGPKKSYLISLIGSYMKMAYKTWNKDHFVSDEGILK
                     DLKALSKGKVHVEDETSLDVLSRRTKGGPQNLQPASSENRPVKTGKFRHNRNRRFNDR
                     HGRNNQQQRRRG"
     gene            complement(13104..14438)
                     /locus_tag="PKM27_15925"
     CDS             complement(13104..14438)
                     /locus_tag="PKM27_15925"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNR08808.1"
                     /translation="MRNSIISLFTLFFSLIFIRCNDPAILGSDLLKEDEIDLNYVDTF
                     RLKARTLLDTGVITYDRSISISFNQLPMGRFDDPIFGTVEARIYAQFIKSFFELPPDF
                     KNAVLDSAMVYLALDTVTAPYGKAAEQKHDLEVYPMLESISTTKRYRSGYYSFMNEAM
                     AAAKLYNYFPDPKFRPMVLEPEDTFSYDPNVAFRLDNAYGNYILGLDSLWYNSDSIFL
                     ANVHGLMFRQANANQRMVHYWINSSYSRLQIFYKVGTQARIYRFPFQGGPRIPYWQHD
                     YSPIIQEALNTPETGDQMIFLQSLSGLKAKIEFPTLKLPPGVIINKAELEVTALTLPG
                     DDPAVFTPPGQLGLLRFDDAGNEVFISDQGALDDFTLLGGTLTTKTVNGASLSTYTMN
                     VSNHIQEIILGKQAPECYLAIFPRSEKLSRVVLGGPQHPQYPMKLRISYTTQ"
BASE COUNT         3673 a         3550 c         3824 g         3456 t
ORIGIN      
        1 acaccaccct gatctcgggc tggattatga ttggaattta ttccgggagc tcaaacggat
       61 gtatcccagg ttgaaactgc atgccctggg ccctcccgaa atagctcata tttccaaaat
      121 ttccggcacc actcatctgg agaccctgac cgccctgaaa gaggccggac tggattcttt
      181 ccccggggca ggagctgaaa tcctggtcga ccgggtgaga aacctgatcg cccggggaaa
      241 atgcctcagc caggagtggc tggatgtcat gcgggcggcc caccagctca acctgaccac
      301 ttccgcgacc atgatgtttg gtacggtgga gacgattgaa gaaaggatgg agcacctggt
      361 ccgcatccgg gaggttcagg cagaaaaacc tgaaggggcc accggtttcc tggcctttat
      421 tccttggcct ttccaggatg aaggaaccct gcttcagcgt ttaaaaaggg ccgaaaataa
      481 taccaccgcg gaagagtata tccggatgat cgccctgagc aggctcatgc tccccaacat
      541 caccaatatc caggcttcct ggctgacggt tgggaaatcg gtagcacaga tctgcctcca
      601 tgcaggggcc aacgatatgg ggagcatcat gatcgaagaa aatgtggtgt ccgccgcagg
      661 ggccccccac cggttcaccg ccaagggaat ccaacaggct atccgggagg ccggcttcga
      721 accccagctc cggaatcagc attatcaaaa aagggagatg cccgaaaata tggaagaaca
      781 gaggatagaa cccgtggggc tcagggtaaa ctgaggtttt ttcaggacgc cgctctgcat
      841 tactattaat attaatctct gctttctttt attgatacaa ataattgaaa atcaataata
      901 aaagatccag gaatcaacag ttggttggaa gtgctatccg aataaatcgc ttaaaagatc
      961 attgaccttg ccgagggcgg tcacctgaat tttattttta gttttagaga gacccttcag
     1021 attgttggaa gaaatataga tgcggttaaa acccagcctt tccgcttcct ggatccgctg
     1081 ttcgaccctg ctgaccgccc tgatctcccc gctgagaccg acctccccgg cgaaggcgat
     1141 atgcttatca atggccatat cctgaaggga ggaaaccagg gccgccacta ccgccagatc
     1201 aagggcggga tcattgattt tcagtcctcc ggcgatattg agaaatacat catagcttga
     1261 gaaccgcagg cccacccttt tctccagtac ggccaggagc atgctgaggc gccggaggtc
     1321 aaaggtggtg gccgagcgct gggcggttcc gtataacgcg gtgctgacga gggcctggac
     1381 ctccagcagg acgggccgct ggccttccac ggtggcgcag accgcgcttc cgctcaggtt
     1441 ttcctgggtc tgggacaaga ggagttcgga agggttgtgg atctggcgta ggccgttggc
     1501 ctgcatttca tacaaaccca tttcgtccgt ggatccaaac cggtttttac gggttcgcaa
     1561 gatcctgtag gcgtggaagg tatccccttc gaactgaagc actacatcca cgatatgttc
     1621 gagcactttt gggccggcga tcatgccttc tttattgata tgcccgatca ggataaaagg
     1681 aattccgctt tgttttgcat aacgctggat ttctgcggta cattccctga cctgggtaat
     1741 gcttccctgg atggattcca ctgttggcag ggtcagggtc tgaatggagt cgatgatcac
     1801 caaatcgggc tggagggcct ggatttcatc gaggacccgg ctgagttccg tctcggttag
     1861 aatataacag tggttggggg taaggcccat gcggtcggcc ctcattttaa tttgttcttc
     1921 actttcctcg ccggagacat agaggatttt agctttcagg ttgagggcaa gctgcatcat
     1981 gagggtagac ttcccgatcc cgggctggcc tccgatgagg atgagggaac cttgcacgat
     2041 tcctcctccc agggcccggt tgagttcatg gtccggggtg atcagtcgct ttgcggctga
     2101 tttttggatt tcagaaagta aaaccggttt tgctcccggt ttttgggaac gtagggactt
     2161 ttgaagggcg tcgccgggtt tggacacgtc cacgatctcc tcgacataag tattccattg
     2221 atcgcaagaa gggcaatgac cgatccattt cgggcttttg gccgcgcaat ttgaacaata
     2281 gtatactttt ttcagtttca tataacaatg atcattgccg ggtctaattt aaatcatccc
     2341 taaggctaat ccgggtcaaa attattttat tttatcgagg ggatctttga aagagaaatt
     2401 gattaaaaag agattaaaaa aaacagggat aaagatcctg ccggatttaa tcaaaatatt
     2461 gatcaaaaca ggcaactgtt gagatcgaca attggttttt tggggtttat acagggtgct
     2521 ttgctgttct gcagtcaagg tgccctgttt tattttaaac cgttttactc cctgattaca
     2581 aatattgagc ggcaatttta aggccttcca gggtcagatg aggggcatac agattgacac
     2641 ggtcttcaag ggtatggatc attttactca aacccccggt agcgatcaaa aataaatcga
     2701 gccccagttc cttttcctgg gcggagacta tgcccctgac caggccgtcg taaccatata
     2761 gaatgcccgt ctggatggcc tgttccgtgc ttttccccaa ggcggactcc ggcatttgaa
     2821 gcggaaccgt acgcaattgg gcggtatggg tggtgagggc ctggagggcc gtccggaggc
     2881 cgggagcaat gctcaccccc agaatagtcc cggtggcatc gatcgtggtg aaactgaggg
     2941 cggtcccaaa atccaccaca atgctggctc ctttggataa gttatgagcg gccaaggcgt
     3001 tggcaaccag gtcggtgcca atttcatccg gccgaaggat cacaagcggt aatttttcaa
     3061 actggctttt ttccagcact ttaatatctt gtttgctcca ctgagccaaa gccttggtga
     3121 cctgttgggt tgctgcggga accacgctgc tcaccactac ccgttccgga gggcgaaagc
     3181 cgccgaatcc cttttgaagg aattggctga gaagggaagg atcattgatc agcgaagcgg
     3241 tctcgaggcg gtaaaccagg ggagattcat ccccggaatg ccagatgcca aaaacggtat
     3301 ccgaatttcc aacgtctgtg accaggaggt tcataaatgc aaaatatgcc aggtgaagtg
     3361 aaattaggtt aattttatgc ttttaaatac tgcatctgtg gaattacttg aacttcagat
     3421 agaaagcctg atttttattt catccgaacc cctgaaaccg gaggatctga tcgcctgcct
     3481 ggaggcctat cataaagaga aaatcgaacc aaccctggtg atggaatgcc ttgaaaaaat
     3541 aaaaacgaaa tacgcttccg atgattttgc ctttgaactc aatgaaatcg ccgaaggctt
     3601 ccagtttttg accaaggccg cctatcatcc catcgtccgc acctttctga accagatgag
     3661 ccagaaacgg cttagccgca cggccctgga gaccctggcc attattgcct acaaacaacc
     3721 cgtttcaaaa cccgaggtcg aacggatccg cggggtcaat tgcgatcata ccatccaaaa
     3781 actgctggaa aaagaattga tcaccatgga aggtcgggct gaaggccccg ggaaacccat
     3841 actctatgtg acaagtgaaa agtttatgaa ctatttcggc ctcaaaagca ttgatcaatt
     3901 gccgaatatc aaggaattta accatggatt caatgaagtc ggggaggaga tcccgatcga
     3961 acgaatggtc atggacctgg agcaggatat atcggccaat aaaaacaacg aatgaacacg
     4021 gaatccactt tagtcaaccg ggtagcggaa agcgaacttc aaaccatcgc cctggatgat
     4081 atgatccggg atgcggcggt ggtttccttt gacctgaaag attatttgtt tcagggactc
     4141 atcctcaagg agaaagattt ccgggaatcc ctgaaagcca ccgattggtc caaataccgg
     4201 gaccagatcc tcgctgtgca ttgcagtacg gatgccatca ttccgatgtg ggcgtatatg
     4261 ctgatcgctg cttatgccgc accggtggcc aaagccattt attatggcgg aaaacaggag
     4321 gtcctggagc aggtccttct cgaacggata aaccaaaaag actgggagta cctgcgcggc
     4381 aagcgggtgg tgatcaaagg atgcagcgac caggaacttc cggcctcggc atatttgcaa
     4441 atcgtgcgtc agctgcaacc cctggctcaa agcattatgt acggggaacc ctgcagcacc
     4501 gtcccgattt tcaaaaggcc gagggtggtt taaacccaaa ccttatcatt gacctcctca
     4561 tgggggattt gggttaaacc ctgttaaatt ttatattccc ggatattctg gtttaaattt
     4621 gtcttgcaca gggcctgatg aaacaatagg ttttatcagg agagtttctt tggttttcta
     4681 tttatccaaa aattcagacg gtatgaataa gttgatgcat gtgtattctt ttgtgctggc
     4741 cggtttgttg tcaggcctgt tgtttacttc ttatgtaaag cccgaaaaag gcggcgcctc
     4801 cgggaccggg gaggataagg aaacgaaaat tccacaggcg attcatgccg tccccatccg
     4861 ggaaaaatac agcttcggcg gagaaaatgt tcccatacag aatttcgatg tccgggagcg
     4921 gctggaccgg gaattgctgg tcaatactta ttggcattcc aattccatcc tttgcattaa
     4981 agccgctcag cggtacttcc ccgtcataga ggaggtcctg gccaaataca ggatcccgga
     5041 tgacttcaaa tacatccccg tgatcgaaac ctccctgcga aacgaaacgt caccggccgg
     5101 ggccaagggg atctggcaat ttatgaagtc cgttgccgaa tcctacgacc ttgaggtgaa
     5161 tgaggaagtg gacgaacgat atcattttga aaaagccacc gaggccgcct gccgccacat
     5221 cctgagatac aaacaacgct tcggaagctg gaccctggcc gcggccgcgt acaatgcagg
     5281 agaaggcaat ctggccaaag aactcggccg ccagggtgaa cgctcctatt atgacctcaa
     5341 tatcaatgat gaatcttcaa ggtatatttt ccgcctcctg gccatgaagg acatcatgga
     5401 gcacccggat gactatggat tttttatcca gccggaagaa aaatacagcc gcaacaacac
     5461 caaaaaaata gaagtgaaga ccgctgttcc caaccttggg gactgggcca gggaaaatgg
     5521 gaccacttac cggatgctca aattgatgaa cccctggctg agaacttcaa gccttaccaa
     5581 taagctgaaa aaaacctatt atatcgaagt accgaaataa atcaacggcg cccgagcaat
     5641 cccgactgct tggatttgaa ggcttcataa gcgcaatcca ggaattgctg ataccccttg
     5701 acactgcttc gatagcccct ggccgggata agcacctcct gatcgttggg atccaccggc
     5761 acatacaacg gctgggaatt ttgctggaaa ttgacaatct ggaagtcttc ccatttattg
     5821 ccgatgttcc ggatcttttt gtccctgctt tttgaataga tcacctcatc cagcggcgtt
     5881 tcatcatcac aatacaggct aaccagtaca aaatcacccg ccagcctgct ccagatttcg
     5941 tcctcgaccc atatgtgttc ttcggtcttg cggcagttaa cgcaaccgta acccgtaaaa
     6001 tccagcaaga tgggtgtttg aacttccttg gcgtaaacca ggccttcctg gtaatcttta
     6061 aaacaatcca ggttgttggc gcatttggaa aaggaggaaa acctcgcttt caggtccgga
     6121 tccgcctctg gaagcggtga aaaataattg taataagccg gaggggccag cccgctgagc
     6181 agggagagcg actggtaggt ctttgttttt tcattaaaaa ggaaaccggt agccaggtac
     6241 agggtccaga cggccgagag ggcggcaaaa gcggtcctca gggtgcttct ttttttcatg
     6301 gggctgtcgt gagggaattg gaaccaaccc atcagatagg cggtcatcgc agcaaaaatg
     6361 agtatccaga gtcccataaa caattcatat ttcaggattc cccagtgcga ggtcatgtcg
     6421 gaaaccgaaa ggaatttcag ggccagcgcc agttccagga agccaagcac cactttcacg
     6481 ctgttcatcc agctgcccga ccggggtaag gattgcaacc aggcgggaaa agcggcaaat
     6541 aatccaaagg gcaaggccag ggccagggaa aatccaaaca tgacgacggc ggggccgact
     6601 ttgctggtgg ccgaagaaac cagggctgtt cctatgatgg ggccggtgca ggaaaaggaa
     6661 acgatggcca gggtgaatgc cataaaaaag atgccgagga ggttgccgct gcgtccaagg
     6721 gcatcggtct tggtgctcca gctgctgggc agggtgatct cgtaataccc gaaaaaggaa
     6781 aaagcaaaga ccacaaaaat gatgaaaaag agcagattgg ccagccagtt ggtcgagagg
     6841 tcattgaggg cggtttcccc gaagaacgag gtgatcagca ggccgagccc cacatagatc
     6901 acgatgatcg ccagcccgta caacaccccg ttgcgtatgc ctgttgcacg gttatggctg
     6961 ctcttggtaa aaaaactgac cgtcagcggc aacaaaggga agacacaagg ggtcagtaag
     7021 gcaagcaatc cccccaggaa tccgaaaata aaagtcagga gcagggagac gtctattttt
     7081 ttttcttcga cggcacagtc gctggtcacg gtttggtagc tggactgaag gctgctgacg
     7141 gactggtcca acagcttttt ttccgggctt ccttcctgtg ctgttttttc cgccggttgg
     7201 gcaagtcccg gtaccgtgaa tgaaaaatca taatcaaacg gtatgcattg tacgtcgtcg
     7261 catacgatcc cgtccacata gcccgtgatc gcctcccccg ggcggagaat ctcgacaacc
     7321 tgtacaaatt gggcttcttc ggttattttg accacctctt cttcaaagac tttgtcgaaa
     7381 ctttttttga tatggcccga ttcttcattt ttccccagga aacgcagggc tgatgaatct
     7441 tcaaaatgga attcgatggg tttcaccgag gtttctgcac tttcctgata ttgactgtaa
     7501 gtgccccagc ctttgattat ttttaattca aagatcaacc ggtaccgcga cgagtccagt
     7561 tgttcaatcc ggtgtgacca ttctacgggt ttggagtcgc cgatgccgat ttgcagaaaa
     7621 ctccagagca gggtaagcaa caataccatg aaaattattt taccgaagtc aggtcaatgg
     7681 tgaaagggac ctctttggga ggcaggcatt gcagttcgtt gcaacacatg tactctatga
     7741 tccctgtgat gggttttgaa gggtccagga ctttaacttt ttgggtaaag gtgccttttt
     7801 tgctgatttt gatgacttcc atgtcaaaca tttcatcgtg cccttttttt atagttcccg
     7861 attcctcatt tttaccaacc agggcaaaat ggggcccggc atcaaaggtg aaggaggtcc
     7921 tgaccggccc gtcatcggag gcgaggtact gggagtaaat gttccagccc tcttcgatgg
     7981 ccgccgtgaa tatgatttta tattcctgcg cacctgtctt ttccactgaa tgactccagg
     8041 acacgggaac cttgccggag ttaaaaaaca aactcagaag gatcaggaca gtcatggtca
     8101 aaattttctg cgaaattaag acttgtgcag ccggcctatt tccgattaag aaataattaa
     8161 ctcccggcca cgggattagc ggccaccagg ttatggatca tggagcggaa gaggcccagg
     8221 ccgtcctcgt tgcctagcat ggcttcagaa gccctttcgg gatgaggcat catgccgtaa
     8281 acattccggt gaatattaca gatgccggcg atattcaaca aggaaccgtt gacattagcg
     8341 gcttcgcttg ggtttccctc cgcatcacaa tatttaaaga ggatctggtc gttttggatc
     8401 aattggtcga gtacctcctg gtctgcataa tagcgaccgt cggcgtgtgc gatgggcatt
     8461 cgcagaacgg tccctttcgg cagggcggag gtgatcattg aatttgaagt ctgtaccctc
     8521 aggtgcacgt tgtcgctgat gaatttctgg tcccggttgg ccaggagtac accgggcagc
     8581 attcccgctt cgcaaaggat ctgaaaaccg ttgcagatcc cccagacaaa accgccctga
     8641 tcggcgaaat ctttgatggc gggcatgatg ttggcataac gggctatggc tccggatctc
     8701 aggtaatccc cataggagaa cccccccggc aacaatatcc cgtccaggtc cgggagctta
     8761 tggtctttgt gccagatttt gagggtatgc accttgcaga cttccccaac gacatggacc
     8821 aggtcgtcat cacaattgga acccgggaag acgacgacgc cgaatttgag gtcatgcata
     8881 tcaggccaaa gttacaaaat aaaagagctg gaaaaggatg accccgagaa aaaggataaa
     8941 gtggatggtt tccgccaggg tttggttttt caggtgggag atggccaggc cggcgaatat
     9001 gccgagcatg ggggctccca gcagcaatac gttccagcca aagggttgat gaaacagccc
     9061 taccaggaca gagtgaaaaa gggcgaaata gaggatatcc atttttttct gtgcctggat
     9121 gtgttttttc ttcctgaatt cattgtaaag aagaatggaa aaaatcagga ggaaaaggaa
     9181 aaagccccct ttgagcatgt ccgcggtggt ggagccgatt tccagtacat tcagccccat
     9241 gtgattgcca aaagaaggaa acacttccag gccgtgtccc cgccaaaatt tccaggtgaa
     9301 ccataaaaac cagggcaagg ccccgccaat gaggatctgg atcgggtgta ccaactccat
     9361 ttttttgaga ttgttcaccc cgatgaatat caggaacagg taaaggatcg ttggcggaca
     9421 gaagatggag gcaatgccta tcaggaaaga agcattaaag atctcaacgg ccggactgga
     9481 ggttttatag atctggaaaa gctcatgcag ggcccagatg accataaaat tggccaggtg
     9541 gaggtgggaa aacgaccaga attcaggcag gatggaactg tacaggataa aaaacacgcc
     9601 cgggtaaagc ataatgtccc ggtgcagtct gttttctatg accatgcggt tgatgaatat
     9661 ggcctggata aaaagcatca ggatatgaag cagggaagcc agaaacggcg aacggacgat
     9721 gtccgaagga gggattctgc cctgtcccag cgctgcggga atgatctgca gtaaaaaggt
     9781 ataagccaac agcagcagac tgtttataaa accatttttc ctgaagaggg acagcatgaa
     9841 gtgcacaaaa ttaaaacaat aaacacgtac ttggtctttt gggccctccg gaattgtccc
     9901 ggggccccgg ccgggcggcc aacaaaaaac tcagcctata aacgggccaa accacaatat
     9961 tggtatcttg tgcgcgaggt gaaattatgg aaaaggatct cctggcaatt caaagaccga
    10021 ttgaaaaaga actgggtctt ttcgaggttt acttccgaaa tgccatgaag agccaggttt
    10081 ccctgcttga ccggatcacc tattatattg tcaaaagaaa ggggaaacaa atccgcccga
    10141 tgtttgtctt cttcagtgca aaactggtgg acgaaatcca accggcaacc tataccgcgg
    10201 cggctttggt ggaaatcatc cacaccgcga ccctggtcca cgatgacgtc gtggatgatt
    10261 ccatgcaaag gaggggtttt ttctccatca acgccctttg gaaaaataag atcgcggtac
    10321 tggtcgggga ttacctgttt gccaaggggc tggatatttc aatccatgag aaacaatatg
    10381 aaatcctggc catcctcaac aatgccatta aggagttgag tgaaggagaa ctcctgcaga
    10441 tcgaaaaagc caggaaactc gatatcaatg aacaggttta ttacgagatc atccggcaga
    10501 aaaccgcctc cctgattgcc gccagttgtg cggcgggggt ggcttccgtc acgaaagatc
    10561 ccgggatcat tgcccgggtc aaacaaatcg gggagtggat cgggatggcc ttccagatca
    10621 aggatgacct ctttgattac ggcgaagcct ccatcggcaa gcccaccggc atagacatta
    10681 aggaaaaaaa gatgaccctg cccctcattt ataccctcca gcatgccggc gggggcttga
    10741 aacgggagat catgaacacg atgcgccatc acagcgagga ccgcaggaaa gtaaaagaag
    10801 tgatcgaggc ggtacggtcc aacgggggac tggaatacag cacccggatc atgcacgaat
    10861 acgccgataa ggcaaaaaag gaattgttga ctttccccga ttccacggcc agggccagcc
    10921 tcgcttccct gattgatttc accatcaacc ggaaatcata acctgcggat gcgggcccca
    10981 atggcattca aacggccatc gatattctga taaccgcggt cgatctggtt tacgttgtgg
    11041 atcaggcttg ttcctttggc gctcagcgcc gccagcaaca gcgccacccc tgcccggatg
    11101 tccggggagc tcatttcgat cccgcggaga aaatgtttcc ggtttaatcc gatcacggtg
    11161 gcccggtgcg gatcacagag gatgatctgg gcccccatct caatgagttt atccacaaaa
    11221 aataggcggc tctcgaacat tttctggtgg atcagcaccg atcccttgct ctggatggcc
    11281 attaccagga tcacgctcat aaggtcaggt gtgaaacccg gccagggcga gtcatagact
    11341 gtaaggatgg agccatccat aaaggtggag atctcgtatt cgtccagcgc cgggatatag
    11401 aggtcatcac ccttttcaat cagcgtgacc cccatcttac ggaatacgtt cgggatgatg
    11461 cccaggtttt ggatcccggc ctgctggatg gtgatttcag aacgggtcat ggcagcgagt
    11521 cctataaaac tgccgacttc aatcatgtcg ggcaacatcc ggtgttcggt gcccttcaaa
    11581 accggcaccc ccgtgatggt cagaagattt gaaccgatcc cttcaatatg ggcccccatc
    11641 cggaccagca tcctgcacaa ttgttgcaca taaggctcac aggcggcgtt gtaaaccgtc
    11701 gtggtgccct gggccagtac ggcggccata accacgttgg ccgtaccggt cactgaaatt
    11761 tcttccatgt ggatataggc cccgttcagg ttttttgctt cgataaaata ctgggctttg
    11821 gccggatcgt attccacttt cgccccgagc tgggttaatc cttcaaaatg ggtatccagc
    11881 ctgcgcctgc cgattttatc gccccccggt tttggcaggt acatcttgcc cagcctggca
    11941 agcaggggcc cgataagcat gacggatccg cggatggctt tggctttttt gtcaaattca
    12001 tcgctggcca tatattcccg gtccaggtcc gccgccgtaa aggcatacgt cccttccgcc
    12061 agtttttcca ccttcacacc caggcctccc agcaattcaa tcagccgggt cacatccagg
    12121 atccccggaa tgtttgacac cgtcaccttt tcgcgggtga gcaggacggc gcacaaaatc
    12181 tgcaaagctt cgtttttggc tccctggggc accagggttc ctttcaactg acaatttccc
    12241 tggacttcaa atgcatctgt gatcatggcc tggattcgat tcggctcaaa tgtattaaaa
    12301 ataattgtaa tatgaaattg atcagatacg agaaacgagc tacgaggtac gagccacgag
    12361 cttccagctt cgagcttcga gcttcgagct tcgaggtcct acccccttct cctctgctgc
    12421 tgattattcc ggccgtgccg gtcattgaac ctgcggttcc ggttatgcct gaatttgccg
    12481 gttttgaccg gacggttttc ggaactggcg ggttggaggt tttggggacc tccttttgtt
    12541 ctgcggctga ggacatccag ggaagtctcg tcttccacat gcactttacc cttgctcagg
    12601 gctttgaggt ctttgagaat gccttcatcg ctcacaaaat ggtctttgtt ccaggttttg
    12661 taagccattt tcatatagga tccgatgagg gagatcaggt agcttttttt aggcccgtct
    12721 tccatctggc tggctttttc aatcagtctg cgcacatgat tgccataatg gcggtaggtt
    12781 tcgcaggtgg gcggataatc caccggcacc ggtttgatat tgctctcttc cgcttcgtag
    12841 cggatgccct caaaggatac gacatcgatt tgatattcgg ctatggcaaa aaaatgtttc
    12901 cacagtttcg gccggatatc gctgccgttt ttcgggcctg gggcgagatg ccccatcagg
    12961 ctgacgatgg cattggccat ggcttgtctt ttgcggggat cttcctcatt ccggatcgaa
    13021 cggatcatct cctggacgtt gcgtccgtat tcggggtaaa ggagcaggga gcgggtcgaa
    13081 ttgtaattca tgccttttaa taattattgg gtggtataac tgatgcgcaa tttcatggga
    13141 tattggggat gctgaggtcc ccccaggacc accctggaca gtttttccga tctgggaaaa
    13201 atagccaggt aacattcggg agcttgtttt ccaaggatga tttcctggat gtggttggaa
    13261 acattcatgg tgtaagtact gaggctggcc ccatttactg tttttgtagt gagggtaccg
    13321 cccaggaggg taaaatcatc cagggcgccc tggtctgaaa taaacacctc gttgccggca
    13381 tcatcaaagc ggagcaggcc gagctggcca ggcggggtaa agaccgcggg atcatcgccg
    13441 ggtaaagtta aggccgttac ttccaattcc gccttgttga tgatcacccc gggcggaagt
    13501 ttcagggtcg gaaactcaat tttcgctttc aaacccgaaa gcgactggag aaagatcatc
    13561 tggtctcccg tttccggggt gttcagggct tcctggatga tcgggctgta atcgtgctgc
    13621 cagtaaggga tcctgggtcc gccctgaaaa gggaaacggt aaatcctggc ctgggtcccg
    13681 accttataaa atatttgcaa gcgggaatag gaggaattga tccagtaatg caccatacgc
    13741 tgattggcat tggcctgcct gaacatgagt ccatgcacat tggccaggaa aatcgagtcg
    13801 ctgttgtacc agagcgagtc cagtcccagg atataattgc cgtacgcgtt gtccaggcgg
    13861 aaagctacgt tcgggtcata gctgaaggtg tcttcgggct ccaggaccat cggccgaaac
    13921 ttagggtccg ggaaataatt ataaagtttt gcagcggcca tggcttcgtt cataaaactg
    13981 tagtagccgg accggtaacg cttagtggtc gatatggact cgagcatggg atagacctcc
    14041 aggtcatgtt tttgttcggc agcctttcca tagggggccg tgacggtgtc gagggcaagg
    14101 taaaccatcg ctgaatccag gaccgcattt ttaaaatccg gcgggagctc gaaaaaggat
    14161 ttaatgaact gggcatagat cctggcctcc accgtgccaa atatcggatc gtcgaaacgg
    14221 cccatgggaa gctggttgaa agaaatggag atggaccggt cataagtaat cacccccgta
    14281 tccagcaggg tcctggcctt aagtcggaag gtgtcgacgt agttcaggtc gatttcatct
    14341 tccttgagca ggtcggatcc gaggatggcc gggtcgttgc acctgataaa aatcaggcta
    14401 aaaaacaggg tgaacaggga aattattgaa tttctcattc tgaatgcttc ttgaccccgg
    14461 aagtaattat ttgagcaggg tttcgtagaa gtgaagatag gcc
//
LOCUS       DAOQXT010000110        14391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4678_length_14391_cov_7.514230, whole genome shotgun sequence.
ACCESSION   DAOQXT010000110 DAOQXT010000000
VERSION     DAOQXT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14391
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4678_length_14391_cov_7.514230"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2320)
                     /locus_tag="PKM27_15930"
     CDS             complement(<1..>2320)
                     /locus_tag="PKM27_15930"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08809.1"
                     /translation="MNTCDQTITVQDTTRPTITCPTDTLVICFTDVPAVDLNAVLSTD
                     NCTQIVIITHVGDSIGDSTCINGLTVFRTYRATDSCGNFNECIQQITVLDTEAPQFTY
                     FPNDTLVVCASDLPIQTPLASDNCGLVRVSHVEDIVTDSGCINQFTLWHIWEAVDTCQ
                     NMIRDTQMVVVRDTILPQFTYFPNDTLVNCMTDLPIQMPQAADNCGLVRVSLVEDIIT
                     DSNCINQFTLLHIWEAVDTCQNMVRDTQVVIVRDTLLPAFTFFPNDTTVACAADITIQ
                     VPEASDNCGPVRVSLVEENTIDSSCVNQKTRLFIWEAVDTCQNAIRDTQFVFVLDTVG
                     PEIPVPGNIEVVQCGTFFSIVLGIVATDNCDGMVPVDADISIIDSTCPGDYKILIVYT
                     SNDTCGNFSEVRDTIIVIDTLDPVITCPGDYTLGCEGGLPAPITSLDSFFLAGGQIYD
                     SCCLDSSSFRFIQEILDCDADGDPTLSRYYAIDDCCGHSDTCVQVITIPPCLLDLALR
                     KTLNAPEPYFAGYGGTVPFTITVYNQFCVPADSIKIIEYLPSVGSSVTDPGWVNNGDG
                     TACLLLTSNDLLPLGGLLYGDSVVISFTVQLGLDFGDGTGINRAEIFSANTPDGPASD
                     VDSDPNNNPGDDIIVDDVIDNSGSDEDDYDIAQFFVCQPLVCNNHVNVSLEEDCELCF
                     TADDFLEGGALPEEFYTIELFAGGKKLPGNCVDRAYLGYTLTYSVLINSPCGNNSCWG
                     TVTIEDKAPPMIDCRADTVLCFELSNLPSEGMV"
     assembly_gap    2321..2420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2421..3179)
                     /locus_tag="PKM27_15935"
     CDS             complement(<2421..3179)
                     /locus_tag="PKM27_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08810.1"
                     /translation="MKSKISKVVSAFIITLVVASAMTVYRTGWHWPGQVDRTKGTLSV
                     TGNNASGAFGFEHQIKTVSTEVFAFLLQPVITCPAPDTVDCDSPLDTTALGSPIVVPD
                     VACTMGIDTLFYSDSLADFDCPNTYNLFRTWIVVDSCGLSDTCIQSIRVQDTTRPTIV
                     CPDDITIYRDGSCGIDTTLGTTGSPTIGDNCGTPTLIYSDNTAGLDQCSGTGSIIRTF
                     TVTDSCGNMNTCDQTITVQDTTRPTIVCPDDITIY"
     gene            3660..4271
                     /locus_tag="PKM27_15940"
     CDS             3660..4271
                     /locus_tag="PKM27_15940"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNR08811.1"
                     /translation="MPRSDPRIDAYIDKAAPFARPILDHIRSLVHQACPEVEEKIKWS
                     FPHFDYKGEMMCSMAAFKAHCAFGFWKAELIRSSQGKLQLADRTAMGNLGKITGLKDL
                     PSDRAMIKMIQEACALNDQGIKVKKPEPAQKKEVVAPEYLLQALAKNKAAQKTFDKAS
                     YSFRKEYIEWITEAKSETTRQSRIKTALDWMAEGKGRNWKYER"
     gene            complement(4450..6696)
                     /locus_tag="PKM27_15945"
     CDS             complement(4450..6696)
                     /locus_tag="PKM27_15945"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNR08812.1"
                     /translation="MIHLAWTAPVLRFKGNDAPDRLSLGKIRLLIRTCYLTVVFFAVT
                     LTWKGPAAQTGFNHLKNETSPYLRQHARNPVDWYPWGREALDKAKSEDKLMVISIGYS
                     SCHWCHVMEKEAFSDTAVSAFMNKHFVSIKVDREERPDIDQVYLSACRLVNGDACGWP
                     LNIIALPDGKPVFSGTYMEKKQWLEMLAFFRDQKNSQTDKLVQYGRQLSQRLQQESGN
                     QDGHEGLPDSLFHELVLDFLPNTDTAGGGLQGSPKFPMTPLYEFLLAYHALFGGEGVF
                     RAVESTLDHMMKGGIYDWLEGGFARYSVDEAWTLPHFEKMLYDNARAISLYAQAFRFN
                     GKKEYREVVRACTDFLENQLKAKSGGYFSSFDADSEGEEGKYYLWSLDEVEQALADPG
                     LFEMSKSVFGLSEEGNTLGSGDESLPGKNVLAFFRDPGEWAAAKGWTPEQAEGEMKRV
                     RERLLAARLQRTRPAVDSKIICSWNALVVIAYADAFQALGEKTYKKRALENGEFIWKY
                     FVQKDGSLSRLYDQKVKKGNAFLEDYAYTGLSYVRLYQISFEEKWLKRAQTLANYALR
                     HFYDSSRHAFYYTSGLEPALVARTLDEEDGVLPSSNAAMATLLFQLGTYFDDGEFLRI
                     SGKMVTKSMSRMREEGMKASFAHWSLLYIQTGSRPLYEIAVVGKNSHELSRRLASRYI
                     PNALLLGGKRGGKLDLLQGKYVPGKTLIYVCQNKVCQLPVEQVEDAWRILDIKNLNTL
                     HYVPAPAP"
     gene            6760..8256
                     /locus_tag="PKM27_15950"
     CDS             6760..8256
                     /locus_tag="PKM27_15950"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08813.1"
                     /translation="MYSIRMINVSVTGLILILGTFIILPATSQTETSWTKNLPGIGTF
                     SSTRVADLNGDGTDDIVLGAGREETQTCDSGVIALNGKTGELMWKVFARDQMFGSAAF
                     QDIDADGHPDVFISGRSSELIAISGYSGKVIWRFDPKSREHRKQRWFNFYNPQWIPDQ
                     DGDGLQDLLVSNGGDVQVEAYNPNRAPGYLLILSARTGAILHQAVMPDRKEIYMSVCI
                     SPSPGSPDFDIVFGTGGETLGGHLFLGKLSQLLRNDLSEAVILDSTADRGYIAPAARA
                     DLNGDGVTDILVNAVNGRLIAFDGMSLGRLWEVKRPGTETYSSITVGYFNRDEVPDAF
                     CSYAMGSWPKMDWSAQFMADGKTAKVEFEDSLGFYQNSSGIAVDWDGDGYDEVLLSVN
                     FQEIKNLYQKFFYNMLVLIDFKTNEVLQIGETFDGNNISSTPWAGDLDHDGYLDIVYV
                     HGTNLRHTYTFDGMQVHRIATKVPVKKPVRWGAYQGSAYTGIFPNGSK"
     gene            8289..9059
                     /locus_tag="PKM27_15955"
     CDS             8289..9059
                     /locus_tag="PKM27_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08814.1"
                     /translation="MNEHTALKPVSGHSTKGVRSFFGPIIGSFYILVFSLAAALPVGA
                     QNDVHVGRNLFDHLLVKSKEAIPRIDRSEIEGSPYLNDSFTLAMVLTTDAIVHNLPVK
                     YDIYNDLMEFKDRDQVFLLDPSPKIQKILLEKQTFVVEPYDHDGKFGPGYFILLDSVK
                     VKLLQKKVIRFQELQPAKALQTQNTPAKYVPMPDKFYYRIGTEEAKPINNLKKMIAQF
                     PSHQKALLAFANRRNLGKTKEDLLQLWEYYHGLEERPN"
     gene            complement(9064..11187)
                     /locus_tag="PKM27_15960"
     CDS             complement(9064..11187)
                     /locus_tag="PKM27_15960"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNR08815.1"
                     /translation="MPIRLKVLFSALSICLLVSASAQPAGSPDKTFPWWKKNSLRVIQ
                     VNLPAYEAATLDPDSLVQDLVTHAANAVIINAGGIMAFYPSKLDFHYINPYMKPEMLG
                     KVVENCHRNGIKVIVRFDFSRIDRSIFEAHPDWAYVDRNGEQMINTDKYVTCINAPYM
                     QEKAFEIIAEVIRLYPVDGIFQNMPGYQVNNAYIGKYHGIDQNEHDKKRFYDFSGGMT
                     LPLEENPRDPVFVKYQEFKKFTTEEWQDRLHKLVKSMNPNIAICTYATKNVDMIRHES
                     QTNTLPYFPYNASDNVNVMMTSYPDHIVSNASIQQISFQSRYNAIEPEEMEIRLWENI
                     ANGSGLDMSMMGDLRNYEDERNFSVMKKVYGHHRKFEKYYGRYRSLARVAVVAPGWWP
                     HGNTMQEYRGIQLMLQEAHIPFDRIEDSQLGARREDLKKYSLIILPDIHTLNPESADA
                     LESLAREGISLVATNQTLRERPDLLNKWFGVRPKNRVEDCSGQYLEVPQDGRFPSLPG
                     QKMIFLKFNLGEYDMISCDEYLMPLLSKGRPGPPEMIGGHDSTGYYGIGLKKYTGAMN
                     AILPLNLGKLYYLHGYEQHKHLFLDLLRRLLPSLDQDMMTNAHPRIETILREFDYNEE
                     AAFGKPFISRGRILHLINLSGFSGNTYFEPMAASGLFFEFPCEFIPTRVWTMSGQKVS
                     FKTEKGKLKLDLAELQNYEGIVIER"
     gene            11413..12255
                     /locus_tag="PKM27_15965"
     CDS             11413..12255
                     /locus_tag="PKM27_15965"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNR08816.1"
                     /translation="MISRRKFLYQSSISALSVPFLHEDMTGIPKHRRNIGVQLYSVRV
                     EMNQDAKGTLAKLAAMGYNQIESARSQKGLYYGLSPKEMKQSCMDLGMTLRSGHAHVD
                     KDWLKTIDQAAEAGQEYLICSTMPTSGQTVSNYQEVADTFNKAGEQCKSRGIKFGYHN
                     HEYEFDTVDGQVLYDILLKNTESGLVHMELDLGWVVAAGKKPLDYFNRFPGRFPLWHL
                     KDMNLTEKKSTEFGKGQLDIPAILKSHKKSALKYFFIEQEEYGKSAFDSLDYDIQYYK
                     KVKW"
     gene            12757..13098
                     /locus_tag="PKM27_15970"
     CDS             12757..13098
                     /locus_tag="PKM27_15970"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNR08817.1"
                     /translation="MDGTINISNSKAQLRKGVLELCILAIIHEQPVYPSDIIKRLKAS
                     ELIVVEGTLYPLLNRLKDAGYLEYNWQESRSGPPRKYYQITDHGRQLYQELYQSWKEL
                     VHSVNQTTNNQ"
     gene            13098..>14391
                     /locus_tag="PKM27_15975"
     CDS             13098..>14391
                     /locus_tag="PKM27_15975"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNR08818.1"
                     /translation="MNKIHTINLGGHPYTVDEDAFEYLHAYIRSLKKHFKSSPGCDEI
                     INDIESRLAELLQERMQDKAIVTLREVKEVIAIMGTPEEFGAEPIDENKFDPEKPRKE
                     RSGGRLFRDPDDKIIWGVCSGIAAYFGIQDPVWVRLVFGLMILGGGIGLPLYIILRII
                     MPKADSAADRLAMRGEPIDYQSIAKKIQDEIEHLSGEFNNPMDPESKQQWDAGWNAAG
                     KGIRTGANLLSGVFQFIGNILERIVPLALKIAAVVSIAILCFFIVSMIFGWSLLWPYA
                     GYLVPGVRAASALAMMNLFFLGIIPMVFIIFLFARMYNGTRLPRSLAAGLAGLWLLNL
                     MGGILFGVNTARQFSNHAEINQLVYEGSINQDILNIELGDSKERDDMFTLFGEAWIED
                     HSIVLPRIPVHIVESPDQQFHVSQNNNASGQTSREARLR"
BASE COUNT         3706 a         3567 c         3547 g         3471 t
ORIGIN      
        1 accatccctt ccgaaggcag gttcgacaat tcaaaacaca agaccgtgtc cgcgcggcag
       61 tcgatcatcg gcggcgcctt gtcttcgatt gtaaccgtac cccaacagga attgtttcca
      121 cagggggaat tgatgaggac cgaataggtc aaggtatacc caaggtaggc cctgtccacg
      181 caattgcctg gcaatttttt accgccggca aagagctcaa tggtataaaa ttcctcgggc
      241 agggcacccc cttcgaggaa atcatcggcc gtgaaacaca attcgcaatc ctcttccagg
      301 ctgacattga cgtgattatt gcaaaccaag ggctggcaca caaaaaactg cgcaatgtcg
      361 tagtcgtctt catccgaacc cgaattgtcg atcacgtcat cgacgatgat atcgtccccg
      421 ggattattgt tggggtcgct gtccacgtcg gatgcagggc catcgggggt attggcggaa
      481 aatatttcag ccctgttgat gccggtccca tccccaaagt ccaatcccaa ttgtacagta
      541 aatgaaatga ctaccgaatc tccgtatagc aaaccaccca gaggcagcag atcattgctg
      601 gtcaggagta aacaggccgt cccgtcaccg ttattcaccc acccggggtc ggtcacggag
      661 gatccgacgg acgggagata ttctataatt tttatactgt ctgcaggaac acagaactgg
      721 ttataaaccg taatggtgaa tggaaccgtg ccgccataac cggcgaaata gggttccggt
      781 gcattcaatg tttttctcaa tgccaggtcc agcaaacaag gcggtatagt gatcacctgg
      841 acgcaggtgt ccgaatgtcc gcagcagtcg tctatggcat aataccggct cagggtcgga
      901 tcgccgtctg catcgcaatc caggatttcc tgaatgaagc ggaaagaaga ggaatccagg
      961 caacaggaat catagatctg accccccgcg aggaagaagg aatccaggct ggtaatggga
     1021 gcgggcaagc cgccctcaca tccaagggta tagtctccag gacaggtgat gaccggatcg
     1081 agggtatcga tcacaatgat ggtatccctg acttcggaaa agttgccaca ggtatcattg
     1141 gaagtgtaaa caatcaatat tttataatct ccgggacagg tactatcaat gatggaaata
     1201 tccgcatcta ccggaaccat accgtcgcaa ttatctgtgg cgacaatccc cagaacaatg
     1261 ctgaagaaag ttccgcactg gactacttca atattgccgg ggacaggtat ttcaggccca
     1321 acggtatcca aaacaaatac aaactgagta tcccggatcg cgttttgaca ggtatccacg
     1381 gcttcccaga taaacaacct ggttttttga ttgacacagg acgagtcgat ggtattttct
     1441 tccaccaggc tcacccgcac cgggccgcaa ttgtccgaag cctccggtac ctggatcgta
     1501 atgtctgcag cacaggctac ggtggtatcg ttgggaaaga aagtgaatgc cgggaggagg
     1561 gtatcccgca cgatgactac ctgggtgtcc cggaccatgt tttgacaggt atccaccgct
     1621 tcccagatat gcaataaggt aaattggttg atacagtttg agtccgtgat gatatcttcc
     1681 accaggctca cgcgcaccag gccacaattg tccgcggctt gaggcatctg gattggtaaa
     1741 tccgtcatgc aattaaccag ggtatcattt ggaaaataag tgaattgggg taaaatagta
     1801 tctctcacca ccaccatctg ggtatcccgg atcatatttt gacaggtatc cacagcctcc
     1861 cagatatgcc ataaggtaaa ttggttgatg cagccggaat ccgtaacaat gtcctccaca
     1921 tggcttaccc ggaccaggcc acagttgtcg gaggcaaggg gggtctgtat tggcagatcg
     1981 gaagcgcaga ccaccagagt atcatttggg aaataggtga actggggggc ctccgtatcc
     2041 aaaacggtga tctgctggat acattcattg aaattaccac aagaatcggt ggcccggtag
     2101 gtcctgaata ccgtgaggcc gttgatacag gttgaatccc cgatggaatc gcctacatgg
     2161 gtgatgatta cgatttgtgt acagttgtcg gtgctcaaaa cggcattgag gtctaccgcg
     2221 ggaacatcgg taaaacaaat tacaagagta tctgtaggac aggttatcgt cggccgcgtc
     2281 gtatcctgca ccgtgatcgt ctgatcacag gtattcatat nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn gtatatcgta atatcatcag ggcaaacgat cgtcggccgc
     2461 gtcgtatcct gcaccgtgat cgtctgatca caggtattca tattgccgca ggaatccgtc
     2521 acggtaaagg tccgtataat acttcccgtc ccgctgcatt gatccaggcc ggccgtatta
     2581 tcactataga tcagggtcgg agtaccgcaa ttatccccaa tcgtcgggct gcccgtcgtc
     2641 cccagtgtgg tatcgatgcc acaggaacca tcacggtata tcgtaatatc atcagggcaa
     2701 acgatcgtcg gccgcgtcgt atcctgcacc cggatacttt gaatgcaagt atctgatagt
     2761 ccgcaggaat ccactacgat ccaggttctg aataaattgt aggtattggg gcagtcaaaa
     2821 tcggcaagac tgtcgctata aaaaagtgta tcgatcccca tcgtacaagc cacatcgggc
     2881 accactattg ggctgcctaa tgcagtggta tccaaaggtg aatcacagtc caccgtatct
     2941 ggtgccggac aggtaattac gggttgtagc aaaaaagcaa atacttctgt ggatacggtc
     3001 ttgatttggt gttcaaatcc gaaggctcct gaagcattat ttccggttac tgaaagcgtc
     3061 ccttttgttc gatccacctg gccgggccag tgccagcctg tccggtagac ggtcatggcc
     3121 gaagcaacga ccagggtgat gataaatgct gaaacgactt tagaaatctt acttttcatg
     3181 cagcttgaaa tttgaaatca gggagtaaat aatttttttc ggaaagggta taggactgga
     3241 ttaaacggca aatccaaggg ttcgaatgcc tgatccagct attcatataa ttttttttta
     3301 gatgctttat caattggcta ttaattattt aaataatgaa tttcgagata tccataattg
     3361 gtcaataaat aagccaataa ccatgccgat taatcttaat ttgaaggtca ctgggctaaa
     3421 agaaaaaatg gattcatgga aggaatgacc gcctttacaa atcaattgtc ctgtttacca
     3481 aattttaaaa atccattcaa atggctggat aaggtcatgt catattaaat aatttggtat
     3541 gtaaaagtca ttgtatacaa atcttatata aaacaatttt tatcatataa ccgtttttgc
     3601 acaactggat tatgcgaaaa gccattttct tctgatttta gattccaaat cgaaaccaca
     3661 tgccccgctc cgatccccgc atcgatgcct atatcgacaa ggcagccccc tttgcccgcc
     3721 ccatcctgga ccatatccgc tccctggtcc accaggcttg cccggaggtg gaagaaaaaa
     3781 taaaatggag ttttccccat tttgattaca agggggaaat gatgtgttcc atggccgcct
     3841 tcaaagcgca ctgtgccttc ggattctgga aggccgaact gatccggtcc tcccagggca
     3901 aactgcaact ggccgaccgt acggccatgg gtaaccttgg caagatcacc ggccttaaag
     3961 acctgccctc cgaccgggca atgatcaaaa tgatccagga agcctgtgcg ctgaatgatc
     4021 aggggataaa ggtcaaaaaa ccagagccgg ctcaaaaaaa agaagtggtc gctcctgaat
     4081 acctgctcca ggcgctggcc aaaaacaagg cggcccagaa aacctttgac aaggcatcgt
     4141 attccttccg aaaagaatac atcgaatgga tcaccgaagc caaaagcgaa accacccgcc
     4201 agagccggat aaaaacggcg ctggattgga tggcggaagg caaggggagg aattggaagt
     4261 atgagcggta atcatataaa tttctataaa ttctttcccc ccctgacccc tccagggaac
     4321 cacagatctt ggctgtaatt taaaaatttt ggatttggcc ctgcacttta tataaaatca
     4381 atcagggcga gatcaatcag ggcgagacta aaatggattt tcccgaatac caaactaatg
     4441 gactcccctt cagggggcgg gggcgggaac ataatgtaac gtgtttaaat tttttatgtc
     4501 caatatcctc caggcatctt ccacctgctc caccggtaat tggcagacct tgttctgaca
     4561 aacataaatc agtgttttac ccggtacata tttgccctgg agcaagtcca gcttaccgcc
     4621 ccttttccca cccagcaaaa gggcgttggg gatataccgg ctggccagcc ggcggctcaa
     4681 ctcatggctg tttttaccga ccacggcaat ttcatataga ggccgggagc ctgtctgaat
     4741 ataaagcagg ctccaatgtg caaaacttgc cttcatgccc tcctccctca tccgggacat
     4801 ggactttgta accatttttc ccgatatacg aaggaattcc ccgtcgtcaa aataggtccc
     4861 caactgaaac agcaaggtgg ccatggccgc attggaagaa ggaaggaccc catcctcttc
     4921 gtcaagggtg cgggcaacca gggccggctc caggccggaa gtgtaataaa atgcatgccg
     4981 ggaggaatca taaaaatggc gcagggcata gtttgccagg gtttgggccc gttttaacca
     5041 tttttcctcg aagctgatct ggtagagccg tacgtaggag aggccggtat atgcataatc
     5101 ctcaagaaag gcatttcctt tttttacttt ttgatcataa agcctggaga ggctcccgtc
     5161 tttctgcaca aaatatttcc agataaactc cccattttcc agggcccttt ttttatacgt
     5221 tttctcgccc agggcctgga aggcatcagc ataggctatc accaccaggg cattccagga
     5281 acagatgatc ttggaatcca ccgcaggcct tgtcctttga aggcgcgcgg ccagcaatcg
     5341 ttccctgacc cgtttcattt ctccttccgc ctgttcaggg gtccatcctt tggcagcagc
     5401 ccattcaccg ggatccctga aaaaagcaag cacatttttg ccggggaggc tctcgtctcc
     5461 actgcccagg gtattccctt cttcactcag gccaaaaacc gattttgaca tttcaaataa
     5521 accgggatcc gccagggcct gctccacttc atccaggctc cagagataat attttccctc
     5581 ctccccttcg ctgtcggcgt cgaaggatga aaaatatcct ccgcttttcg ccttcaactg
     5641 gttttcaagg aagtccgtgc aggcccggac cacttcccgg tattccttct taccattaaa
     5701 ccggaaagcc tgggcataca ggctgatcgc ccgtgcattg tcataaagca ttttttcaaa
     5761 atggggcagg gtccatgctt catcgaccga gtagcgcgca aaaccaccct ccagccagtc
     5821 gtagatcccc cctttcatca tatgatccag ggtggattcc accgccctga agaccccctc
     5881 ccctccgaag agggcatgat aagccaggag gaattcatac aagggcgtca tggggaattt
     5941 agggctgccc tgcaagccac ccccggccgt atcagtattg ggaagaaaat ccaaaaccag
     6001 ttcatgaaat aatgaatccg gcaggccttc gtgcccgtcc tgattcccgg actcttgctg
     6061 caacctttgg ctgagttgcc ggccatactg caccagttta tcggtttgcg aatttttttg
     6121 gtccctgaaa aaagcgagca tttccagcca ttgttttttt tccatataag taccggaaaa
     6181 gaccggtttt ccatcgggca gggcgatgat gttcaaaggc cacccgcaag cgtccccatt
     6241 gaccaaacgg caggccgaga gataaacctg gtctatgtcc ggcctttcct ccctgtcgac
     6301 cttgatggat acaaaatgtt tgttcatgaa ggcgctcacg gcggtatccg aaaaagcctc
     6361 cttttccatg acatggcacc agtggcagga tgagtatccg atgctgatga ccatcagttt
     6421 gtcttccgac ttcgccttat ccagcgcctc cctgccccag ggataccagt ccacggggtt
     6481 tctggcatgc tgtctcaggt aagggctggt ctcatttttc agatggttga agccggtctg
     6541 ggcggccggg cccttccagg tcagggtgac agcaaagaaa acaacggtta aataacaggt
     6601 acggatcaaa agccggattt tacccaaaga taaccggtcc ggggcatcgt tccctttgaa
     6661 acgaagtacg ggtgctgtcc aggcaagatg aatcatcctt atcccgggat agaatttttt
     6721 aaaaatgagg caaagggacc atttaaaccg tttatattca tgtattctat cagaatgata
     6781 aatgtttcag taaccggtct gatcctgatt ctgggcactt tcatcattct tcccgctacc
     6841 tcacaaaccg aaacatcctg gaccaaaaac ctgccgggga tcgggacttt ttcctccacc
     6901 cgggtagccg acctcaatgg agacgggacg gatgatatag tcctgggtgc aggaagggaa
     6961 gaaacccaga cctgcgattc gggggtgatc gccctgaacg ggaagacagg ggaactgatg
     7021 tggaaagtct ttgccaggga ccagatgttt ggttctgcgg ctttccagga catcgacgcc
     7081 gacggccatc cggatgtgtt tatcagcggc cggtccagtg aattgatcgc catttcgggc
     7141 tacagcggca aggtgatctg gaggtttgat cccaaatcgc gggaacaccg taaacaaagg
     7201 tggtttaatt tctataaccc ccaatggata ccggaccagg acggagacgg attgcaggat
     7261 ctcctggttt ccaacggcgg cgatgtccag gtggaagcct acaaccccaa ccgggcgccg
     7321 ggttatctgc ttatcctcag cgcccgcacc ggcgccatcc tgcaccaggc tgtgatgccc
     7381 gaccgcaaag aaatctacat gtcggtctgt ataagtccaa gtcccggttc ccctgatttt
     7441 gatatcgtgt tcggcaccgg cggggaaacc ctgggaggac accttttcct gggtaaactt
     7501 tcccaattgc tccgcaatga cctcagtgaa gcggtgatcc tggacagtac ggcggaccgg
     7561 ggttatattg cccctgcggc ccgggccgat ctcaatgggg atggggtcac ggatatcctg
     7621 gtgaatgcgg tgaacggccg cctgatcgct tttgacggaa tgagcctcgg gcgcctctgg
     7681 gaggtgaaac gtccggggac agagacctac agctccatta cggtgggtta tttcaaccgg
     7741 gatgaggtgc cggatgcttt ttgttcctac gccatgggca gttggcccaa aatggactgg
     7801 agtgcacagt tcatggccga cggtaaaaca gcgaaggtgg aattcgagga ttcactcggg
     7861 ttttatcaaa acagttcggg cattgccgtg gactgggacg gggatggtta tgatgaggtt
     7921 ctgctgagcg tcaattttca ggaaataaaa aacctctatc agaagttttt ctacaacatg
     7981 ctggtcctga tcgattttaa gaccaatgaa gtccttcaaa tcggggaaac tttcgacggc
     8041 aacaacatct cttccacccc ctgggccgga gaccttgacc acgacggcta tctggacatt
     8101 gtctacgtgc acggcaccaa tctccggcat acctacacct ttgacggcat gcaggtccac
     8161 cgcattgcta cgaaggtccc ggtcaaaaaa cccgtacgat ggggtgcata ccagggcagc
     8221 gcctataccg gcatctttcc taacggatca aaatagcccc acatggatta ttccggtatc
     8281 tttataagat gaacgaacat actgctttaa aaccggtaag cggacactct acaaaggggg
     8341 tccggtcttt cttcggacct atcatcggaa gtttttatat tttggttttc agtcttgccg
     8401 ccgcccttcc ggtcggggcc caaaacgacg tgcacgtcgg ccggaacctg tttgaccatc
     8461 tattagtgaa gagcaaggaa gccataccca ggatagaccg ctccgagatc gagggaagtc
     8521 cttatctcaa tgattctttt accctggcca tggtcttgac cacggatgcc atcgtgcaca
     8581 atctcccggt caagtatgat atatataatg acctgatgga attcaaggat cgggaccaag
     8641 tgttcctgct ggacccctcc cctaaaatcc agaagatcct tctggagaaa caaaccttcg
     8701 tcgtggaacc ttacgatcat gacggaaaat tcgggccggg atattttatt ttgctggatt
     8761 cagtcaaggt caaactgctt cagaaaaaag taatccgttt ccaggaactg caacccgcca
     8821 aagccctcca gacccagaat accccggcca aatatgtgcc tatgccggat aagttttatt
     8881 accgcatcgg tacggaagaa gccaaaccca tcaataacct caaaaaaatg atcgcccagt
     8941 ttcccagtca tcaaaaggcc ttacttgcct ttgccaatcg acgaaatctg ggaaaaacga
     9001 aggaagacct gctccagctc tgggaatatt accacggttt agaagaaagg ccgaattagg
     9061 gtcttatctc tcaatcacga tcccctcata attttgcaat tccgccaggt cgagtttcag
     9121 tttgcctttt tccgttttga aggaaacttt ttggcctgac atggtccata ccctggtggg
     9181 aataaattca caaggaaatt caaaaaacag gccactggcc gccatgggct cgaaataagt
     9241 attgccgctg aaccctgaca ggttgatcag gtgtagaata cgtccgcggg atataaaagg
     9301 ttttccaaaa gcggcttctt cgttataatc aaactcccgg aggatggttt cgatccgggg
     9361 atgggcattg gtcatcatgt cctgatccag ggaaggtaga agacgccgga gcagatccag
     9421 gaagaggtgt ttatgctgtt catatccatg gaggtagtac aactttccca ggttgagcgg
     9481 gaggatggca ttcatcgcgc cggtatattt tttcagaccg atcccgtagt acccggtgga
     9541 gtcatggcct ccgatcattt cgggagggcc gggcctgccc ttggacagga ggggcatgag
     9601 gtattcatcg caggagatca tgtcgtattc gcccaggttg aatttgagaa agatcatttt
     9661 ttggccggga agggagggaa agcggccgtc ctgaggtacc tcgaggtatt ggccgctgca
     9721 atcctccacc cggtttttgg gcctgacccc aaaccattta ttcaacaagt cgggccgttc
     9781 ccgcagcgtt tgattggtag cgaccagtga aattccttcg cgggccaggc tttccagcgc
     9841 gtccgcactc tccgggttaa gcgtatggat gtccggcaat atgatcaggg aatacttttt
     9901 aagatcttcc ctcctcgctc cgagttggct gtcttctatg cggtcaaagg ggatatgggc
     9961 ttcctgaagc atgagttgga tgccgcggta ttcctgcatg gtgttgccgt ggggccacca
    10021 accgggcgcc actaccgcca ctcttgccag ggagcggtaa cggccataat atttttcaaa
    10081 ttttcggtgg tgcccgtaca cttttttcat cacgctgaaa ttgcgttcat cttcatagtt
    10141 tcttaaatcc cccatcatgc tcatatccag gccggagcca ttggctatat tctcccagag
    10201 ccggatttcc atttcttccg gttcgatggc gttgtaacgg ctctggaacg aaatctgctg
    10261 gatgctggca ttgctgacga tatgatccgg gtaactggtc atcatgacgt tgacgttgtc
    10321 cgaagcgttg taagggaagt aaggcagggt attggtctgg gactcatgcc ggatcatgtc
    10381 gacatttttg gtggcatagg tacagatggc aatgttggga ttcatggatt tcaccagttt
    10441 gtgaagccgg tcctgccatt cttccgtggt gaatttttta aactcctggt acttgacaaa
    10501 aacagggtcc cgggggttct cttcaagggg cagggtcatg cccccgctga aatcataaaa
    10561 ccttttttta tcgtgttcat tctggtcaat gccatggtat ttgccgatgt aagcattgtt
    10621 gacctggtat ccgggcatgt tttggaaaat gccatccacg gggtaaaggc ggatcacctc
    10681 cgcgatgatt tcaaaagctt tttcctgcat gtacggcgca ttgatacagg tgacgtattt
    10741 gtcggtgttg atcatttgct ccccgttgcg gtccacatag gcccagtccg ggtgcgcttc
    10801 gaagatggaa cggtcaatcc tgctgaaatc aaaccggacg attactttga tcccgttccg
    10861 gtgacaattt tccaccactt tcccgagcat ttccggtttc atgtacggat tgatgtagtg
    10921 aaaatcaagc ttgctgggat aaaacgccat gattcctccc gcattgatga tcacggcatt
    10981 agctgcatgg gtgaccaggt cctggaccag gctgtcggga tccagggtcg ccgcttcata
    11041 ggcgggcagg ttgacctgga ttacccggag gctgtttttc ttccaccatg ggaaagtctt
    11101 gtccggtgaa cccgccggct gggcggatgc ggaaacaagt aaacaaatcg acaaggccga
    11161 gaacaaaact ttcaatcgaa taggcatggt gctcatttca aggtataaat tataaaaact
    11221 atgggacttc aaatttgtcc cggatacata aaacaatgca ggggccgtga tccctctccc
    11281 attgtccatt ttgtagtaac cagggataat tgcgtataaa taaagcagaa agaataattt
    11341 ttgtttaaac aaaacattca aaagcatcaa gggtaagcgg ctaaagttta attttgaaca
    11401 aaaccgatgt tcatgatctc ccgtagaaaa ttcctttacc aaagctcgat atccgccctg
    11461 tccgttccat ttctccatga ggacatgacc ggcattccca aacaccgccg gaacatcggg
    11521 gttcaattgt attccgtccg tgtggagatg aaccaggacg ccaaagggac gctggctaaa
    11581 ttggcggcca tgggatacaa ccagatcgaa agcgcccgct ctcaaaaggg tttatactat
    11641 gggcttagtc ccaaagaaat gaaacaaagc tgtatggacc tgggtatgac cctgcgcagt
    11701 ggccatgccc acgtcgataa ggactggctt aagaccatag accaggctgc agaagccggc
    11761 caggaatacc tgatttgttc caccatgccc acctcaggac aaaccgtgag caattaccag
    11821 gaagtagcgg atactttcaa taaggccggc gagcaatgca aatcccgggg cattaaattc
    11881 ggttatcaca accatgagta tgaatttgac acggtggacg gtcaggtgtt atacgatatc
    11941 ctgctgaaaa acacggaatc cggtctggtc catatggaac tggacctggg atgggtcgtc
    12001 gcagccggta aaaaacccct tgattatttc aatcggttcc cgggacgctt ccctctctgg
    12061 catctcaagg acatgaatct cacggagaaa aaaagcaccg aattcggaaa gggccagctt
    12121 gatattcctg cgatccttaa aagccataaa aaatcagcgc tcaaatactt ttttattgaa
    12181 caggaagagt atggcaaatc cgcttttgac agcctggatt acgatattca gtattataag
    12241 aaggtgaagt ggtgagcgaa cgagtaacga gctacgagta atgagctgcg agtaacgagc
    12301 tacgagtaac gagctacgag taacgagctg cgagtaacga gctacgagta aagagctgcg
    12361 agtaacgagc tacgagtaac gagctacgag taacgagcgg cgagctatga ggcctttttg
    12421 aagagggcag ctggctgata aggtcctgat tggaggcaaa ctgaaagggt ctatattcgg
    12481 tgacaaggat ccggactatt tggccaaagc attttagatc cggcaaccgg acaagcatcc
    12541 gtattctttt cgaccaacga tttctcttct gctataaaaa ccaccttttt tatgatgaaa
    12601 gaggagtatt aaaacaaatt caattaatga atataaaaat atttaatgct ttttatattg
    12661 ttaaacaatt gattacaata ttggcattta aaggttcttt gttaaaatta gtactatgca
    12721 tcacataata tctgtcttga cctatatatt tgtcccatgg atggtacaat taatatttca
    12781 aacagtaaag cccagctccg caaaggggtg ttggagcttt gtatcctcgc tatcatacat
    12841 gaacaaccgg tctatccatc ggacatcatc aaaagattga aagcttcgga gctcatcgtg
    12901 gtagaaggca ctttatatcc cctgctcaac cggctgaagg atgcggggta tctggaatac
    12961 aactggcagg aatcccgttc gggcccaccc cggaaatatt accagatcac cgatcacggc
    13021 aggcagcttt accaggagct gtatcaatcc tggaaagaat tggtacacag tgttaatcaa
    13081 actacaaaca atcaataatg aataagattc ataccataaa cttagggggc catccttata
    13141 ctgtcgatga agatgcgttc gaatacctgc atgcttatat ccgcagtttg aagaaacact
    13201 tcaaaagttc accgggatgt gatgaaatca tcaacgatat agaaagccgg ctcgccgaat
    13261 tgcttcagga gcggatgcag gacaaggcca tcgtcaccct gcgggaggta aaagaagtca
    13321 ttgccatcat gggcactccc gaagaatttg gagccgaacc gatcgatgaa aataaattcg
    13381 atccggaaaa gccccggaaa gagagatccg gaggaaggtt gttccgcgac ccggacgaca
    13441 agatcatttg gggggtatgc agcggcattg cggcttattt cgggatccag gatccggtat
    13501 gggtgaggct ggtattcgga ctgatgatcc tgggcggcgg cataggtctt ccactttata
    13561 tcattctccg gatcatcatg cccaaagccg attccgctgc cgaccggctg gccatgcgcg
    13621 gggagcccat cgactatcaa tccatcgcca aaaaaatcca ggacgagatc gagcatttgt
    13681 cgggtgaatt caataacccg atggatcccg aaagcaagca acaatgggat gcagggtgga
    13741 atgcagccgg caagggaatc cgtaccgggg caaatcttct cagcggcgtt tttcaattta
    13801 tcggaaatat cctcgaacgg atcgtcccgc tcgccctgaa aatcgcagcg gtggtaagca
    13861 tcgccatttt gtgctttttt attgtaagca tgatcttcgg ctggtccctg ctttggccct
    13921 atgccggcta tctggtcccc ggggtgcgcg cggcctccgc gctcgccatg atgaacctgt
    13981 ttttcctggg catcatcccg atggtcttta tcatattcct gtttgcccgc atgtacaacg
    14041 gcacccgcct gcccagatcg ctggccgcag gcctggccgg cctttggctg ctcaacctga
    14101 tgggcggcat tttgtttggt gtcaataccg ccaggcagtt tagcaaccat gccgaaatca
    14161 accagctggt gtacgagggg tcaatcaacc aggacatcct gaatatcgaa ttgggcgaca
    14221 gcaaggaaag ggacgatatg tttacccttt tcggcgaagc ctggatcgag gaccacagca
    14281 ttgtattgcc gcgcatcccg gtccatatcg tcgaatcgcc cgatcagcag tttcacgtca
    14341 gccaaaacaa caatgcttcc gggcaaacca gccgggaagc caggttgagg g
//
LOCUS       DAOQXT010000111        14070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4811_length_14070_cov_5.614413, whole genome shotgun sequence.
ACCESSION   DAOQXT010000111 DAOQXT010000000
VERSION     DAOQXT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14070
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4811_length_14070_cov_5.614413"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1360
                     /locus_tag="PKM27_15980"
     CDS             110..1360
                     /locus_tag="PKM27_15980"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNR08819.1"
                     /translation="MKIITWTAFLFFISNHFYAQSQMLKAGAFAVNITPSRPVPLSGY
                     AGRAQPSTGVHDSIYARAFVFDDGQTRACLIQADLIGFSFEFADEINQEVEKNTGIPK
                     QNSFLVAAHNHGAPSTRAYGESATENLALYLSELKSKLVDAAVRAWGSRVPVNTAYGE
                     GSCRMNINRRARHSEGGIWLGRNPDGPCDHTVGVLRLDNMNGETIALLVNWPCHATVN
                     GQENSLVTGDWPGSTVRYLEKNYKAGIPIGMFAGASGDINPVYGPHKNFGEMDAIGLI
                     LGEEIVKITSALSPQPAGKLAVLSQELTLPGKQRSTGRMMPNESLLPGDSVKLRLSVI
                     KIGTLALTGISGELMTEIGMEIKNNSPFRQTQVITHCNGNSGYLCTDAAYREGGYEPM
                     VSRTMPGIGRIIVDNMVKMLNSLY"
     gene            complement(1445..3166)
                     /locus_tag="PKM27_15985"
     CDS             complement(1445..3166)
                     /locus_tag="PKM27_15985"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNR08820.1"
                     /translation="MQVGLARVDITPETSIRLAGYAARSKGESEGVLQKLEAKALAFG
                     SDAQRPSILITVDLVGIPGHITTKLAEKLSAKLGIDPAQLVICASHTHSGPEVGNLLN
                     ILQYRGATAYSDSLLELGQLKHIAQYIDHLSMKLEELSQAALKDRKPALVSWIQGQAL
                     FAKNRRAQGGPEDVALPIMRIASPDGKLRAILVNYACHGTTLPGDVNHLHGDWMGETQ
                     KQIETRYPGVLALVAIGAGGDSNPQPRGKMEHVEMHAREITDQVEKLLTSSQWQALAS
                     PPAGRMKWVNLPFAKVPTIADLIQQTGDKTVKGYYARLALDRIARGQAVPADLKYPVQ
                     VWDFGGDMLMINLAGEVVVDYSIRLKEEIGAEHVWLNAYSNDVPCYIPSKRILQEGGY
                     EADLSMYYYDKPSPFSEAVEDLIISAVHDLLPNAFKNVRVSNNSPVVLKADPGKTIEL
                     RAELARSTGPEIKYMPEWRAFGWFTELDQVEWEVSLKKGGTYEVYLDWSVSDEEAGKP
                     YVFESGNQRINGIVGKTGSWFTYKYEKIGTIKLAAGKSGLVFKAGADSPKGALLDLRS
                     MKLVPVK"
     gene            complement(3360..6368)
                     /locus_tag="PKM27_15990"
     CDS             complement(3360..6368)
                     /locus_tag="PKM27_15990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNR08821.1"
                     /translation="MNSRFLILVQLILIISCKPSENIGKGPVEPGEALATFELEPGYK
                     IECIASEPLISDPVDMEIDEYGRLYVVELHGYPLDLGGSGVIKLLADTDGDGRMDQGT
                     VFADKLVMPFGIMRWKKGVLVADAPNILYMEDTDGDGKADVRDTLLTGFAVSNAQMNV
                     SNPVYGLDNWIYVTSESGSTYQIYKKEFGDLGSDILYPDQPDGPRLPLKGSGRTVRFR
                     PDWRALELTSGKTQFGHDFDSRGHHILGNNSNHIYHEVIADSYLKRNPDLLVSNATQN
                     MTDHGEEVFPITQNPEHQLLTNIGIFTSACGNIAYSGAAFTEAFNKNVFFVCEPVSNM
                     VHADLLVDTGTSFKARRIGRPNKEFLASTDFWFRPVNLYSGPDGALYVVDYYRQIIEH
                     PEWMSEEAIQSGQLYNGSDRGRIYRISKTDAQPAAWMNGLKLGDAADGALVEELSNPN
                     GWWRLNAQRLLIDRGGKEAVPALKQQVQNQSSSLARLHALWTLEGLGALTPDLIEMSL
                     QDRDAGIRENAIRLAESFLKTSPSMFQALLALQNDEDSKVRFQLLCTLGFLDSPASAE
                     TRRNLLFRDLDDQWVQIAALSANRSQTYPLLKEVLGHFQLDRTAYASLAQRLTSMIGT
                     GGDLTRIRSLIHQALGDGDKNYRGWEAPVAEGLARGMERKSFKGSDFKNELPALIKAC
                     FEAPSEPLRNAALHLLSVIKIEDKSELKKGVEQAVGISRDNTLPDGQRAEAIRFLALD
                     ELEPHASLLKSLINPRERQAVQRAALKNLGLIPGHAFTNLVLQEWATLTPELRTEAVA
                     AFMGKPERINHLLNAIDSGRIQKSSIVFYQRVQLMTQPDEKIRTRARALFAENQEDQV
                     TRAYKPALQIKGDASKGKAVYVQNCVICHQVRGALGVSFGPDLGTIHSWPPEGIMANI
                     LSPNLSIAAGFDLWEVELDQGEMVQGLITSETPGAITLKNAEGKQTTINRQDIISLKT
                     LNISAMPAGWEKTINHQQMADLLAFLRQLN"
     assembly_gap    6796..6895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7344..8249
                     /locus_tag="PKM27_15995"
     CDS             7344..8249
                     /locus_tag="PKM27_15995"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNR08822.1"
                     /translation="MTTIKILFVFSMTLSMTECKDGSKPDTSMNPVVFTPANSFTSGA
                     EGPAVDKDGNVYAVNFEREGTIGRITPDGKGSVFIELPNGSIGNGIRFNSSGDMLIAD
                     YTNHNVLKVSMATKELKVHAHEPTMNQPNDIAIDSRDRIYASDPNWAAGTGNIWRIDP
                     DGKVTLLEKDMGTTNGIEVSPDEKTLYVNESVQRKVWAYDLSDSGSVSNKRLLIEFPD
                     FGMDGMRCDQEGNLYITRHGKGTIAVVSPTGALLKEIVLAGKKPSNIAFGGADGRRIY
                     VTLQDTKNMETALVEFPGREWRMRR"
     gene            8863..10251
                     /locus_tag="PKM27_16000"
     CDS             8863..10251
                     /locus_tag="PKM27_16000"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNR08823.1"
                     /translation="MKFNNSSLALAGASWKSAVFLTLSCMVWFRANLIAQSLPSPVFR
                     ASVVKINITPDKPKQLLGYGARVSSGVHDSIYHRIVALDDGRTQFFLVSTEICLMSPS
                     LYDEVAANLKQKLGIEPVNFWWSVTHTHSAPEVGVPGLAEVFLGDRYKHPVDTEYTAF
                     VEKTLFDGIVEARRRLEPARLGIGWGYSQANINRRAVDVDGRASLGLNPDGPVDRRLG
                     LIRLEKLDRSPLALIVNYPIHGTVLGGTSLVISGDAPGVVSDYVEEQTGATTLFINGA
                     AGNIAPIYSVYPSPQAGHLSQFRVLLGDRILEANRMIGHTQDTVRLFTGSLTVESPRK
                     PGLGWPSYLGKYSRTEKNGDPLVRLPVRFLRLNEDLAIWSAPLELFCEISNEVRDRSP
                     FPFTLFYGYTNGWLGYLPAENAWRHGGYEVNTVSPFTPAMPGQLTESVTAYLQGELRS
                     AGSGTSVPNKKP"
     gene            10447..10519
                     /locus_tag="PKM27_16005"
     tRNA            10447..10519
                     /locus_tag="PKM27_16005"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10481..10483,aa:Glu,seq:ttc)
     gene            11193..11423
                     /locus_tag="PKM27_16010"
     CDS             11193..11423
                     /locus_tag="PKM27_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08824.1"
                     /translation="MKIHFSVFLLTAREGLVEGRIKDVNHQLLFDTGTKEEAEAALKK
                     AHIFDPKEKLSGPNWLINSGFRFVILEVFEAS"
     gene            11536..11847
                     /locus_tag="PKM27_16015"
     CDS             11536..11847
                     /locus_tag="PKM27_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08825.1"
                     /translation="MKKSTIYDDILQELKEKGSFPIHNGYDQSTYQALQKLHAWDLIE
                     PSPFNTKYTLTKEGHILLDSGKSFDDLVHPSWKTKTWNVFQKILGPGLVLAFRKLLGL
                     I"
     gene            12003..12659
                     /locus_tag="PKM27_16020"
     CDS             12003..12659
                     /locus_tag="PKM27_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08826.1"
                     /translation="MQETFIILTVVVIIFLSLIVVALIWDIIFRHILNGPSILKMLQI
                     NSSNSTNVPKKSNTDIDDPEYFELDSRHITNLKHLDTILLNILSTGNSARLVDIFPTK
                     SKGVTETIEIFFPGRGHVFLEALDYLEKKGLIYKISNSNLQNHDTYHLTYEGYLKCFG
                     NKLEQEYRNSINEKNMNVLMIKSNLDRNRKSNKLQYLAITISIIAILVSSFIAVLKMK
                     "
     gene            complement(12773..>14070)
                     /locus_tag="PKM27_16025"
     CDS             complement(12773..>14070)
                     /locus_tag="PKM27_16025"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HNR08827.1"
                     /translation="TRFKDENDPFRFVIVCAMWITGFDVPTLSTLYLDKPLKSHTLMQ
                     AIARANRISEGKNNGLIVDYIETYVALLDALAIYGAGDAKGDTEGNQPTEPPVRPLED
                     LIHELDQALIATESYLKLDLKFDLAELIDSTGLDKILAIEKGINAVYTNDESKSKFQL
                     LAREVFNKFKALQPDKVLNQFAPRRNAINAIYSAIEDNIQSADVTEIMKKIQAIVDDS
                     ISSVAAEPGKDNGKIIDLSKLNVEILVKHFEKSGNKNSIVQAIKTKVEQQLRDMVDKN
                     PLRVDFYTRYQEIIDEYNRGKDAVTIEETFKKLIEFVNSLTEEEADTRREGLTDEQKA
                     IFDILRKPSLSDSDKKKVKEIAIELLTELKKDKLKVEQWSDKLETAAAVFNTVSNTLF
                     NSMPYPTYQIDDLELKTNLVYQHLKQRYYGGGQSIYGNY"
BASE COUNT         3482 a         3414 c         3436 g         3638 t
ORIGIN      
        1 gataaaaatg ggaggaagtg ccagtatttg caggcattcc ggtttgtaat agaaaatcta
       61 ttgcatggtt tgggtttatg ctgaattctt ttaccaatcc tttaatcgta tgaaaatcat
      121 cacctggacc gctttccttt tttttatttc aaaccacttt tacgcgcagt ctcaaatgct
      181 gaaggccggg gcttttgcgg taaacatcac tccttccagg cctgttcccc ttagcgggta
      241 tgccggccgg gcacaaccct cgaccggtgt tcacgattcg atttatgcgc gtgcttttgt
      301 ctttgacgat ggccaaacca gggcctgcct gatccaggcc gatctgattg gcttttcctt
      361 cgaattcgcc gacgagatca accaggaagt ggagaaaaat acaggcattc ccaaacaaaa
      421 cagtttcctg gtggccgcgc acaatcacgg cgccccctcg acccgcgctt acggggaaag
      481 cgccacggaa aaccttgccc tttacttgtc ggaattaaaa tccaaattgg tggatgctgc
      541 ggtccgggca tggggaagcc gggttccggt gaacaccgct tatggagaag ggtcctgccg
      601 catgaatatc aaccgccggg caaggcatag cgaaggcggg atctggcttg gaagaaatcc
      661 ggatgggccc tgcgatcata ctgtgggagt cctgcgcctg gacaatatga acggcgaaac
      721 gatcgctttg ctggtcaact ggccctgcca tgccacagtg aatggccagg aaaacagcct
      781 cgttaccggt gactggccgg gatcgaccgt ccggtatctt gagaaaaatt acaaagccgg
      841 tattcccatc ggcatgtttg ccggagcctc cggcgacatc aacccggtct acggcccgca
      901 taaaaatttc ggggaaatgg atgccatcgg ccttatcctt ggtgaagaga ttgtcaaaat
      961 cacttcggcc ctttctcctc aaccagccgg taaactggcc gtgctgagcc aggaacttac
     1021 ccttccagga aaacagcgtt caaccggccg gatgatgccc aatgaatcct tactacccgg
     1081 ggactctgta aaactgaggc tgtcggtgat aaaaatcgga accctggccc tcacgggcat
     1141 ctcgggcgaa ttgatgaccg aaataggtat ggagatcaag aataattctc cgttccggca
     1201 aacccaggtc atcacccatt gtaacggcaa cagcggatat ctgtgcaccg acgccgctta
     1261 ccgggaaggc gggtatgaac cgatggtatc ccggacgatg ccgggtatcg gcaggatcat
     1321 tgtggataat atggtgaaga tgttgaacag cctgtattaa atacgatttg gaaatagcgt
     1381 atgcactcca tccattgtta atcctttata aaatggaaaa ataaaccctt tagccaacaa
     1441 ttccctattt caccggaacc agcttcatac tgcgcagatc aagcaatgct cccttcgggg
     1501 aatctgcccc tgctttaaaa accaagccac tcttgccagc agccaatttg atggtgccga
     1561 tcttttcata cttgtaggta aaccaggacc cggtcttgcc aacgataccg ttgatccgct
     1621 gattgcccga ttcaaaaaca taaggctttc ccgcttcttc gtctgaaacc gaccagtcga
     1681 gatacacttc ataggtccct ccttttttca acgacacctc ccattccacc tggtccaatt
     1741 cggtaaacca tccaaaggct ctccattctg gcatgtattt gatttccggc cccgtcgatc
     1801 tggccagttc cgcccgaagc tcgatggtct tgccggggtc tgccttcaac acaaccggac
     1861 tgttattcga aaccctgacg tttttaaagg cgttgggtaa aagatcgtgt accgcgctta
     1921 tgatgaggtc ttccactgcc tccgaaaagg gggaaggctt gtcgtaataa tacatgctca
     1981 aatccgcctc atagccgcct tcttggagga tcctttttga ggggatataa cagggcacat
     2041 cattggaata agcgttcagc cacacgtgct ccgcaccgat ttcttccttg agccggatgg
     2101 aatagtccac aaccacttcc cccgccaggt tgatcatcag catatcgccg ccgaaatccc
     2161 agacctgaac cggatatttc aagtccgcag gtacagcctg ccccctggca atgcggtcca
     2221 gggcaaggcg ggcataatat cctttcaccg ttttatcccc ggtttgctgg atcaggtcgg
     2281 caatcgtagg gactttggca aaaggcaggt tgacccattt catccggccg gcgggaggac
     2341 tggccagagc ttgccattgg gaagaggtca gcaatttctc cacctggtct gtgatctccc
     2401 tcgcgtgcat ctccacatgt tccattttac cgcgtggctg aggattggaa tcgcctccgg
     2461 cgccaattgc gaccagggca aggaccccgg ggtatcgggt ttcgatctgt ttttgtgttt
     2521 cacccatcca atccccgtgc aaatgattga catcgccggg cagagtggtc ccatggcagg
     2581 cataattcac caggatggcc cgcagttttc catccggtga ggcaatccgc atgatgggaa
     2641 gggctacatc ctcgggcccg ccctgggctc gcctgttttt ggcaaacagg gcctgcccct
     2701 ggatccagga aaccagcgcc ggtttcctgt ccttcagggc cgcctggctg agttcttcga
     2761 gtttcatgga cagatggtcg atgtattggg caatatgttt tagttgaccc aattcaagca
     2821 gggaatcgct ataggccgtt gcgccccggt attggagtat gttgaggagg tttccaactt
     2881 ctggcccgct gtgggtgtgg gaggcgcaga ttaccaattg cgcagggtct atccccagtt
     2941 tggcggaaag tttctctgcc aattttgtgg tgatatgtcc cgggatgccc accaggtcca
     3001 cggtaatcag gatggaaggt cgctgggcgt cacttccgaa agccagggcc ttggcttcca
     3061 gtttttgaag cacgccttcc gattctccct tcgaacgggc ggcataacct gccagccgaa
     3121 tgctggtttc cggagtaata tcaacccggg ctaagccaac ctgcatggtc cctggaggct
     3181 tagcagaggt ttgtgctgtg ccaggaagga tgaaaagaat gaggacaatt aaaagaagac
     3241 cggagatcag agtgaacgtc cccttttgat tttttgaccg ggatatttgg tttggtttca
     3301 taataacgaa attacaaagc atcgaattta ttcaatgaca ttacgggata tgggatgaat
     3361 cagttcaatt gcctcagaaa agccaataag tcagccatct gttggtgatt gatggttttt
     3421 tcccagcccg cgggcatagc ggaaatattg agtgttttta aagatattat atcctggcga
     3481 ttgatcgtcg tttgtttgcc ctcggcattt ttcaaggtga ttgcacccgg ggtttcactg
     3541 gtgatcaggc cctgtaccat ttctccctgg tccagttcaa cttcccaaag atcaaatccg
     3601 gcagcaatgg agagattggg tgaaagaata tttgccatga tcccttccgg tggccagctg
     3661 tgaatggtac ccaggtcggg gccaaaggaa acacccaggg cgcccctcac ctggtgacaa
     3721 atcacgcagt tttgaacata gacggcttta cctttggaag cgtccccttt gatttgaagg
     3781 gccggcttat aggccctggt gacctggtcc tcctggtttt ccgcaaaaag ggcgcgggcc
     3841 cgggtacgga ttttttcatc cggttgggtc atcaactgca cccgttgata aaatacaatg
     3901 cttgatttct gaatcctgcc tgaatcaatg gcatttaaca ggtggtttat tcgttccggc
     3961 ttgcccataa aagcggctac cgcttctgtc ctcagctccg gagtcagagt ggcccattcc
     4021 tgcaatacca ggtttgtaaa ggcatggccc gggatgaggc cgaggttttt taaagccgct
     4081 ctctgaacgg cttgtcgttc ccgggggttg atcaatgatt ttaataaaga agcgtgtggt
     4141 tccaactcat ccagcgccag gaatcggatc gcttcggccc tttgcccgtc aggtaaagta
     4201 ttgtcccggg aaatgcctac tgcctgctcc accccttttt ttaattctga tttatcttca
     4261 atcttaatca ccgataacag atgaagggca gcatttcgaa ggggctcgga gggagcttca
     4321 aaacaagcct tgatgagcgc aggcagttcg tttttgaaat cggatccttt aaacgacttc
     4381 ctttccattc cacgggccaa tccttctgca acgggggcct cccatcctcg atagttttta
     4441 tctccatcgc ctaaagcctg gtgaatgagt gagcggatcc tggtcaaatc gccgcccgtg
     4501 ccgatcattg aagttaagcg ctgggcaagg gaagcataag ctgtccggtc cagctgaaaa
     4561 tgaccgagca cttcttttaa taaaggatag gtctgggaac ggttggcgga caaagccgca
     4621 atctgcaccc actggtcatc caggtccctg aacagcaaat tccttctggt ttcggcggat
     4681 gccggactat ccaaaaatcc aagggtgcag agcaattgaa atctcacttt ggaatcttca
     4741 tcgttttgca gggccaggag ggcctggaac atggaagggg atgtttttaa aaaagattcc
     4801 gccagcctta tggcattttc ccggatgcct gcatccctgt cctgcagcga catttcaatc
     4861 aaatcgggag taagggcgcc aagaccttcc agtgtccata aagcatgcaa tcgggccagc
     4921 gaggaggact ggttttgcac ttgctgtttc aatgcaggta ccgcttcttt tccgccccgg
     4981 tcgatcagta aacgctgggc attcagcctc caccagccat tcggattgga gagctcttcg
     5041 accagggccc catcggcggc gtcgcccagt ttcaggccat tcatccatgc tgccggctgg
     5101 gcatccgttt tggaaatacg gtagatcctg ccccggtctg atccattgta aagctgaccg
     5161 gattggatcg cttcttccga catccattca gggtgttcga tgatttgccg gtaataatct
     5221 accacataga gtgcgccatc cggtccagag tacaaattga ccggacggaa ccaaaaatcc
     5281 gtcgacgcca ggaattcctt atttggacgt cctatcctcc gggccttaaa actggtcccg
     5341 gtatcgacca ggagatcggc atggaccata ttgcttaccg gttcgcatac aaaaaagaca
     5401 tttttattaa aggcttcggt aaaggcggct cccgaatatg cgatgtttcc acaggcggaa
     5461 gtgaatatgc cgatattggt caacaactgg tgctccggat tttgcgtgat gggaaatact
     5521 tcttcgccat gatcggtcat gttctgagtg gcattggaaa ccagtaaatc cggattgcgt
     5581 ttgagataag agtctgcaat gacctcatga tagatgtgat tggagttgtt tcccaggata
     5641 tgatgtcccc ggctgtcaaa atcatggcca aactgggttt tgcctgaagt cagctccagg
     5701 gctcgccagt cgggtcgaaa gcgcaccgtg cggccgcttc ctttcaaggg cagtcggggc
     5761 ccgtccggtt ggtccgggta gaggatatcg ctgcccaggt ccccaaactc ttttttataa
     5821 atctggtagg tggagccgga ttcggaggtg acgtagatcc aattgtccag gccatagacc
     5881 gggttgctca cgttcatctg ggcgttggag accgcgaagc cggtgagcag ggtatcccgc
     5941 acatccgctt taccgtcccc atcggtatcc tccatgtaaa ggatattggg ggcatcggcc
     6001 accaagacgc cttttttcca ccgcatgatt ccaaagggca tgaccagttt gtcggcaaag
     6061 acggtaccct ggtccatcct gccatccccg tcagtatctg ccaggagttt tatgaccccg
     6121 cttcctccga ggtcaagggg gtagccgtgg agttcaacca cataaaggcg gccatattca
     6181 tcgatctcca tgtccacggg atcggagata agcggttctg aggcaataca ttcaattttg
     6241 tagcccggtt ccagttcgaa ggtggccagg gcctctcctg gttctaccgg gcctttcccg
     6301 atattttctg aaggcttaca acttatgata aggataagct ggactaaaat caggaagcgg
     6361 ctattcattt ggttttataa gctcctttca tgagtcagga caaagtagta ttttttaggt
     6421 tcaggtaaaa attgaattca gcggacggat agccccatcc gggatataaa atggggagcc
     6481 gattaaaaat agcggctatt ccgtcccatc tccaccaacc ccgccaggga tgaaattcca
     6541 caggcggagc tgggtgattg ccacctttgc gtcgcgcagg agcggggccg ggtgacaacc
     6601 accgctacgc tgctgcgctg ctgcgctctt ccaatcccgt cagggaagtg atgcgggtag
     6661 tagatccaga agaacggtca ttccgtcccg tcagggatga aattccacag gcagcgctag
     6721 aggtgattgc cacctttgca tcgcgcagga gctaggccag gtgacaacca ccgctgcgct
     6781 gctgcgctgc cacgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggtga
     6901 ttgccacctt tgcgtcgcgc aggagctagg ccaggtgaca accaccgctg cctgctgcgc
     6961 tccaccaatc ccgtcatgga tttgatgtcg gtagcagata caaaagaacg gtcattccgt
     7021 cccgtcaggg atgaaattcc acaggcggcg ctagaggtga ttgccacctt tgcgtcgcgc
     7081 aggagctagg ccaggtgaca accaccgctg cctgctgcgc tccaccaatc ccgtcatgga
     7141 tttgatgtcg gtagcagata caaaagaacg gtcattccgt cccgtcaggg atgaaattcc
     7201 acaggcggcg ctagaggtga ttgccacctt tgcgtcgcgc aggagctaga ccaggtgaca
     7261 accaccgctg cgctgctgcg ctgaagcgct gaagcttgaa gaatcatgta taaatgagat
     7321 atcttgcata tatttaatat aacatgacga caataaaaat actctttgtt ttctcaatga
     7381 ctttgtccat gaccgaatgt aaagatggtt ccaaacccga taccagcatg aatccggtgg
     7441 tctttacgcc ggccaatagt tttacctccg gagcagaggg acctgcggtg gacaaggatg
     7501 gaaatgttta tgcggtcaac tttgaacgtg agggcacgat tggaaggatc accccggatg
     7561 gcaaaggcag cgtcttcatc gaactgccca atggcagcat cggcaacggg atccggttta
     7621 acagctccgg ggatatgctg atcgcagact acaccaatca caatgtgctg aaagtatcca
     7681 tggccacaaa agaactgaaa gtccatgcgc acgagcccac catgaaccag cccaacgata
     7741 tcgccatcga tagccgggac cgcatctatg ccagcgatcc caattgggca gcaggcacgg
     7801 gcaatatctg gcgcatcgat ccggatggca aggtgaccct gttggaaaaa gacatgggca
     7861 cgaccaatgg gatcgaagtc agcccggatg agaagacact ctatgtcaat gaatccgtcc
     7921 agcgcaaggt ctgggcctat gatctgtccg acagcggttc ggtatccaac aagcgcctgc
     7981 tgattgaatt tcccgatttc ggcatggacg gcatgcgctg tgaccaggaa ggaaacctgt
     8041 acatcacccg gcacggtaaa gggaccattg cagtcgtctc ccctacggga gcactcctca
     8101 aagaaattgt cctggccggc aagaaaccca gcaatatcgc cttcggagga gccgacggac
     8161 gccgtattta cgtcaccctc caggacacca aaaatatgga aaccgccctc gtcgaatttc
     8221 ccgggagaga gtggagaatg agacggtagc attgggctaa agcccaatga gagttgatac
     8281 catattttgg tggaatttcc cggtaacccg cacggagctc acggtggcca cggaggaatt
     8341 tttaaatttt cttcaaacat taatttttgg gaaaagcttt agttccgacc ttaaactatt
     8401 acaatctgtt agccctataa atgtggtaga tttccaattt cttggtgtcc ttcgcttcgc
     8461 tgtgtcgttg tgggaccccg ttgtggtaac tgcttgggtg agcgaagctc cgaccttaac
     8521 ctttattcat ccaagagcac tataaatgca gtgggcttcc aatttcttgg tgcccttcgc
     8581 ttcgctgtgc cgttgtggga cttgcttgct tgggaggagc ttagctccga ccgaccttaa
     8641 tcaatatata tgcggtgcac ctctattttc tttgcgccct tcgcttcgct gtgccgttgt
     8701 gggaccttgt acttaactaa gggtaatcgg gagacttcca atttcttagt gcccttcgct
     8761 tcgctgtgtc tttgtgggcg acctttgagg gaaccgcctg ggaggaacga agctccgacc
     8821 ttaaaatatt cattatattg aagacccgat aatccaagat caatgaaatt caataattca
     8881 tccctggcgt tggccggcgc atcatggaaa agtgctgtat tcctcacctt gtcctgcatg
     8941 gtatggttca gggccaatct tatcgcccaa tcgctcccct cccccgtatt cagggcctcc
     9001 gtggttaaga taaacatcac tcccgacaag ccgaaacaat tgctgggcta tggggcccgg
     9061 gtctcttccg gggtccacga cagcatttat caccggatcg tggccctgga cgacgggcgt
     9121 acccaatttt tcctggtctc taccgaaatc tgcctcatgt cgccctcttt gtacgatgaa
     9181 gtggcggcaa acttgaaaca gaagctgggt attgaacccg tcaatttctg gtggtccgtc
     9241 acacacactc attccgcccc ggaggttggg gtgcccggac tggccgaagt tttcctgggg
     9301 gaccggtata aacatcccgt ggatacagaa tatacagcct ttgtggaaaa aacccttttc
     9361 gatggcatcg tggaagcgcg ccgcaggctg gaaccagccc gattggggat cggttggggt
     9421 tattctcagg ccaatatcaa ccggagggcc gtggatgtgg atggcagggc ctccctgggg
     9481 ctcaatcccg atggacctgt ggaccgcagg ctgggcctga tccggctcga aaaattggac
     9541 cgcagtcccc tggccctgat cgtgaactat cccatccacg gcaccgtact cggaggaacc
     9601 agcctggtga tcagcgggga cgcacccggt gtagtatccg attatgtgga agaacagacc
     9661 ggagccacca cactattcat caatggggcc gcgggcaata tcgctcccat ttacagtgtt
     9721 tacccaagcc cccaggcggg gcatttgagc cagttcaggg tcctgctcgg cgaccggatt
     9781 ctggaggcaa accggatgat cgggcatacc caggatactg tgagattgtt taccggttcc
     9841 ctgaccgtcg aaagtccccg taaacccggg ctgggctggc cttcttacct cggtaagtat
     9901 agccggactg aaaagaacgg agaccctctg gtccgtttgc cagtccgctt cctcaggttg
     9961 aacgaagacc ttgccatctg gtcggcccct ctggaattgt tctgtgaaat atccaatgag
    10021 gtgagggaca gatccccctt cccctttacc ctgttttacg gctataccaa tggctggctg
    10081 ggctacctgc cggcggagaa tgcctggagg cacggaggct acgaggtcaa tacggtttcg
    10141 ccttttaccc cggcgatgcc gggtcaactg accgagtcgg tgacggctta cctgcagggc
    10201 gaattgagaa gtgcgggttc gggaacttct gttccaaaca agaaaccctg attggtaccc
    10261 ccctgttggg gcttggaaaa aagaacaata tcgccacacc ctaaaaacga aaatgaagat
    10321 attgtactga tttatagatg cataggtcac catggagagt gcttcaacgg tctgtatttc
    10381 attagttgag ccgaacttta ggataataat aaaattccat tatttttgca cccgctgcat
    10441 taatatggcc cgttcgtata gaggcctagg acgcctccct ttcacggagg aaacacgggt
    10501 tcgaatcccg tacgggctac tgaatcaatt ttattaaacc ctgtagaaga ataatctacg
    10561 gggttttttg tttggacatg gtggtaattt atgtgctcga aagtgaaaag gatggcaggt
    10621 ggtataccgg catggccatt cacgcaactt cccgcttaca tgaacataat agtggtaaaa
    10681 atagatttac caaaagccac cttccttgga agatcattta tcttgaaaat tgtgcagatt
    10741 ggaaggcagt tagaatcaga gaaaagtact tgaagagtgc ggcaggcaaa cgatggttgg
    10801 taaaaatgct taatagcaat gaaaacacgg gttccctgcc cgactgaccg atagaagatt
    10861 taaaagataa actttgctca gttggtcagg cgggcctgcc cgactgaccg ataaatttac
    10921 tatgctcagt cggtcaggcg gggaatcccg tacgggctat actcttggag gatgtacaac
    10981 cagacattcg gtggccttct aaaaaaagag tgctagtcgt tacacttgtt acggttgctc
    11041 cagtttgttt caccctgtcc ccaaaaaagt tcaattgtag gcgaaaaaac ttatataaga
    11101 atttttaaat atgaaaacag atcatgggct gattgctagt aattttgtta aagactaata
    11161 ttggtcaatt ttgtaaagga aagctataag caatgaaaat tcattttagt gttttcctcc
    11221 taactgccag agaggggtta gttgaaggaa ggatcaagga tgtaaaccat caacttttat
    11281 ttgacacggg tactaaagaa gaagctgaag ccgcccttaa aaaagcgcat atttttgatc
    11341 caaaagaaaa gttatctgga ccaaactggt taataaatag cggtttccgg tttgttatcc
    11401 tggaagtatt tgaggcttct taaatggctc tacttcaaaa aatcgtaaaa ttgtattcaa
    11461 agccctgaca cttattgtcg gggtttttta gtaggtcaat ttaacttcag aaaacaaaaa
    11521 aaacaggtta agaaaatgaa aaagtctaca atttatgacg atatactcca agagttaaag
    11581 gaaaagggtt ctttcccaat ccacaatggg tatgatcaaa gcacctacca agcgttgcag
    11641 aaattacacg cctgggacct gatagagcca agccctttca atacgaaata cacacttacc
    11701 aaggaggggc atatactctt ggattccggt aaatcctttg atgacctggt tcatcccagc
    11761 tggaaaacta aaacttggaa cgtatttcag aaaattctcg gtcctggatt agtattggct
    11821 tttcggaagc ttttgggttt gatttgagca ttgtacattg tttttgcact gaaattggac
    11881 cttgagaata taattgttaa ttttaattaa aaaggcatgg gtctgagaga tttaggattt
    11941 cattttgtca tctaagctaa aaggtcagat ccgtgcaatt ggcgttataa ggattaaaat
    12001 atatgcaaga aacatttatt atattaactg tagtagtgat aatattttta tcattgattg
    12061 ttgttgcatt aatttgggat ataattttta gacatattct aaatggtcct tcaatactca
    12121 aaatgcttca aattaattct tcaaattcta cgaacgttcc taagaaatct aataccgata
    12181 ttgatgatcc agaatatttt gaattagatt caaggcatat tacaaatttg aagcacctgg
    12241 atactatttt attaaacatc ctttctactg gaaattctgc aagattggtc gatatttttc
    12301 caactaaatc aaaaggcgtt acagaaacaa ttgaaatatt tttcccgggg agaggacatg
    12361 tgtttctaga ggcattggat tatcttgaga aaaaaggatt aatttataag ataagtaaca
    12421 gtaaccttca aaaccatgat acttaccatc ttacttatga aggttattta aaatgcttcg
    12481 gaaataaact tgaacaagag tatcgaaatt caataaatga aaaaaacatg aatgtcttaa
    12541 tgataaaatc taatctcgat cgaaatagaa aatccaataa gcttcaatat ttagcgatta
    12601 caataagcat aattgctatt ttagtaagtt catttattgc agttttaaaa atgaaatgat
    12661 tcgcactgaa accctattgg tcaatagctt ggtcctgtcc acttcaactt caacgcatcc
    12721 tgcatagcta gcgcgaacga atccatttgc cagtttagct cagtacaaac ttctaatagt
    12781 tcccataaat actctgccca cccccataat acctctgctt caaatgttga taaaccaaat
    12841 tcgtcttcag ttccaggtcg tcgatttgat aggtcgggta aggcattgaa ttaaataaag
    12901 tattgcttac tgtattaaag accgccgccg cagtctccaa tttatccgac cattgctcca
    12961 ccttaagttt gtcttttttc aactcggtta acagctcaat cgcgatctcc ttcacttttt
    13021 tcttatcgga atcggacaga ctgggtttgc ggaggatatc gaatatggct ttttgttcat
    13081 ccgtgaggcc ttcccgcctg gtatcagctt cttcttcggt caatgagttg acaaattcga
    13141 tgagtttttt gaaagtctcc tcgatggtga cagcgtcttt accccggttg tattcgtcaa
    13201 tgatttcctg gtagcgggta taaaagtcca cgcgtaatgg atttttatcc accatgtcgc
    13261 gaagttgttg ctcaactttt gtcttgattg cctggacaat ggagttttta ttgccggatt
    13321 tttcaaaatg cttgactaag atctccacat ttaacttact caggtcaatg atttttccat
    13381 tgtctttacc tggttccgct gctacggatg aaatggaatc atccacaata gcctggattt
    13441 ttttcatgat ttcggtgaca tcggcgcttt gaatattgtc ttcaatggcc gagtagatcg
    13501 cattgatcgc atttctcctg ggggcaaatt gatttaaaac tttatccggt tgaagggcct
    13561 taaacttgtt gaatacttcc cgggccagga gctggaattt gcttttgctt tcgtcattgg
    13621 tatatacggc attgatccct ttttcaatgg cgagtatttt atccagaccg gttgaatcga
    13681 tcagctctgc caaatcgaat tttaaatcca gtttcagata ggactccgtg gctattaatg
    13741 cctgatccag ttcatggatg aggtcttcca gaggtctgac tggtggctct gtaggctggt
    13801 tgccttcggt atcccctttg gcatcgccgg ctccatagat agccagggca tcaagaaggg
    13861 cgacataggt ctcaatatag tccacaatga gcccattgtt tttgccctca ctgatgcgat
    13921 ttgcccgggc aatggcctgc atcagggtat gtgatttgag cggcttgtct aaatataagg
    13981 tagacaaggt aggcacatca aacccggtaa tccacatggc gcatacgatc acaaaacgga
    14041 aggggtcatt ttcatcttta aaccgggtct
//
LOCUS       DAOQXT010000112        13995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4841_length_13995_cov_6.842324, whole genome shotgun sequence.
ACCESSION   DAOQXT010000112 DAOQXT010000000
VERSION     DAOQXT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4841_length_13995_cov_6.842324"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..651)
                     /locus_tag="PKM27_16030"
     CDS             complement(178..651)
                     /locus_tag="PKM27_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNR08828.1"
                     /translation="MARFHFITQWALDARVEEVYEIIKDSSALSRWWPSVYLEVNTIY
                     PGLANGVGKKVALLTRGYLPYTLRWDFEVIQIIPCKKIVLDAFGDLEGRGTWTFSPED
                     KGCLVVFDWDIRFRKKGLAWLSFLLRPVFRLNHRWAMKQGYESIKEEIRRRRMVS"
     gene            complement(905..2251)
                     /gene="mtaB"
                     /locus_tag="PKM27_16035"
     CDS             complement(905..2251)
                     /gene="mtaB"
                     /locus_tag="PKM27_16035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNR08829.1"
                     /translation="MGAPRSIAFHTLGCKLNYAETSTLQSLCEKEGLQVLEFDQPADI
                     YVINTCSVTEQADKKCRYAIRQALNISPQAKVIVTGCYAQLKPAEIVDIPGVSLVLGA
                     GEKFRLTEYLDALAQSNGLSPLQSGTIGEVRTFNSSYSFGDRTRSFLKVQDGCDYKCS
                     FCTIPLARGRSRSDTVEHAVEKAREIAANGVREIVLTGINLGDFGNGTEVIEGMRPKK
                     SALFIDLIRALDEEAGLPRIRISSIEPNLLTDEIIEFVAGSRRFMPHFHIPLQSGSDE
                     LLRLMQRRYVSSLYASRVEYIKSLMPHACIGVDVITGFPGETERHFRETYEFLHRMDV
                     SYLHVFTYSERQNTPAVHLPHSVPVHLRRERNEILRQLSEKKKHFFYQSHLGTSRPVL
                     LEHDGNDRQWCGFTDNYIKVYVDKNAGAPNEIVMARLEGFNGLGEMEGEVIYQFAG"
     gene            2414..2917
                     /locus_tag="PKM27_16040"
     CDS             2414..2917
                     /locus_tag="PKM27_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNR08830.1"
                     /translation="MERNARTFLDVVDSHKGIIYKISAAYCRNEPDRKDLVQEILLQL
                     WLSFHKYNAQFALSTWIYRIALNVSISHLRTEYRRKRINQDLPADLLNLKDEEEPLQP
                     ESDTEFLFACIRQLKAIDRALILLYLEGKSQQEIAEIIGTTPTNVSTRVFRIKQQLKQ
                     KFSNHTK"
     gene            2922..3587
                     /locus_tag="PKM27_16045"
     CDS             2922..3587
                     /locus_tag="PKM27_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08831.1"
                     /translation="MDDHKLLELWKTTDQKLEACLRFNRQNAEDLTKWKVRSLLASMN
                     PLKLFTLLMGILWVCFVDLLIINTFPYGNPFFLVSAGIQVLLTKFAIGIYLYQLILIY
                     QVDVGEPVINTQEKLARLRSSTLWVARLLFLQLPVWTTFYITADMIKDGGAGFFLVQI
                     PVTFAFLYLAIWLFINIKYEKRDKKWFRLLFSGREWGPVIQSMELLDQVREYRQGAPE
                     VKE"
     gene            3663..4322
                     /locus_tag="PKM27_16050"
     CDS             3663..4322
                     /locus_tag="PKM27_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08832.1"
                     /translation="MKRRTIVVILLVVLATALAFDLYLPVKKDFRQFDPAAVGRLDAE
                     MWRSYYEKRPVRLFFQLSRLMRTQFHAPYIRSHFIAYQSAKAAFVFKEGRNRIQYAGA
                     LPYLKTYFSQLNDLSKVPFNFFKLAEEELEWWIIRREGEKFTPSDWEAVLAREGEIMY
                     SIPKENFMDYARDRVAAMVLRDQKGQSITEQDWQTITQMCISAWTKFHAVVQPRISSA
                     P"
     gene            complement(4295..5488)
                     /locus_tag="PKM27_16055"
     CDS             complement(4295..5488)
                     /locus_tag="PKM27_16055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNR08833.1"
                     /translation="MNIYSTVQRKTLLLWAGVLLGLVSVYASWLKYQAPPRAFEGSPL
                     QYTGYNNYVIFKQAFFHLLDHKDMYVLYPAEHHDLFKYSPGFAAAFGILAWLPDCIGL
                     TVWNLLNFLVFFLALTRLSVPWAARIPLLLVFTFPELLTSLQNLQSNGLMTGLLIFAF
                     IDFEKSNTGKAAVWIALAAFIKVYAGLAALFFLIYPGKVRFIIHGLLAVGVLFCLPLI
                     WVPPAEMWAQYRGWWRILSQDHEASLGISILYLIHLVQPAFQSKTFIQVFGLGMILPA
                     LFPLRKFADVLSRINLLAFLFLTMVIYNHKSESATFILAVAGMGIWYFSKANPTRLQT
                     AGIGLAFIFTVLSASDLFPANIRKNWFVPYGVKLWPCILIYLLVLKELILTGFRVQGA
                     EEIRG"
     gene            complement(5485..6993)
                     /locus_tag="PKM27_16060"
     CDS             complement(5485..6993)
                     /locus_tag="PKM27_16060"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR08834.1"
                     /translation="MVMLKRECLCLGFLGLCLVSSGQTILFKNFYIADGTGSKLFLGD
                     LRIRDHKIAILGELIPRPGEKVIDGQNRKILAPGFIDTHSHHDRNLEDSGQYSSFLNQ
                     GITTLVIGQDGSSRFPLQDYFEQLTREGIPVNLASYSGHNTLRFNAMGNTEFNREATH
                     GEIRKMKKGLKQDLKSGALGLSTGLEYDPGIFSSKDEVLKLALAAARKDGRYASHLRS
                     EDVGLEEALTEIIDIGRRTRMPVHISHFKIAMKAKWGMADQLLQRLDSAQTAGVQITA
                     DVYPYDFWLSTLEVHFPKRDFDNRQTAEFALRELTPPDGLILSRYDARPDLVGKSIAQ
                     IAEDWKQEPAEVYMQLIKTARDKNAPEHVMGRSMHENDITRLMQWKHTNICSDGFGGG
                     RHPRGVASFPRVLHYFSGQLKAFEFETAIHKMTLLSADHMGLALRGKISPGFYADLVV
                     MDRDQIKDHASLEDPYALSSGIEQVWVNGVQVWDGRSFMGSRPGMVLKRQAQ"
     gene            7026..7739
                     /locus_tag="PKM27_16065"
     CDS             7026..7739
                     /locus_tag="PKM27_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08835.1"
                     /translation="MGRFYLFIAVFGLLASCNQEDVDQYNYTVRGRVTHEITGLGVPG
                     VWVYTEYGEPCCGSIAFRKGLDSAKTNFEGKYTLQISYPEDTLIYRFITFLKAGTLNN
                     QFYQYSGLDVQDIGLTRFEYNEMVAPVVPAKLPDGHLQSCDFTILPVGYVEIDFRNRG
                     SAPTDTVSVKAIRMDKNQEYKNVLFYPSYSFGLHLFPVPASIPTLLIQENTYNGNTVT
                     VRDTLNLKAGQRVSRTLER"
     gene            7830..8648
                     /locus_tag="PKM27_16070"
     CDS             7830..8648
                     /locus_tag="PKM27_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR08836.1"
                     /translation="MSFLKNKTVFITGGSRGIGKAIGLRMAREGANVALAAKTTEEHP
                     KLPGTIYSAAAEIEAAGGKALPIQADIRFEEQVAAAIHKAVETFGGIDILVNNASAIS
                     LTDTPNTEMKRFDLMHQINTRGTFLTTKYALPFLKKSDHAHILVLSPPIHLDPKWLGM
                     HLAYTISKFSMSMCVMGWAEEFRGKIAVNALWPRTTIATAAVQNLLGGDQMVRLSRKP
                     EIVADAAYEIFRHNASTLSGRFCIDEDILREAGVNDFTSYAVDPGSELMTDLFL"
     gene            complement(9219..9698)
                     /locus_tag="PKM27_16075"
     CDS             complement(9219..9698)
                     /locus_tag="PKM27_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08837.1"
                     /translation="MRTTIFYLAAVLFILTSFTVQPAWKMIGERAVKFSSDHDVIKVG
                     GNDWYTKLKIKVVDAPLHISNFDVVFENGERFDVAIRQDIRKGGESRIIDLPGGERRI
                     DRIEFRYRTTGRVAGGTANVIVFGRLSLPHEGAERVEFARSSSHGEHEERGESLNGR"
     gene            10002..10643
                     /locus_tag="PKM27_16080"
     CDS             10002..10643
                     /locus_tag="PKM27_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR08838.1"
                     /translation="MNHENQITDALAVQEAPVTVVVAGPEHARYAEEICHLIEESAKA
                     RKTGIAKRSPEYIIKKLNSGDAVIALQGEKLAGFCYIETWSHGQYVANSGLIVAPDLR
                     KAGLGREIKIKAFNLARDKYPYAKVFGITTSLAVMKINTELGYKPVTFSELTQDEAFW
                     KGCSSCPNYDILQRNERKMCLCTGMLAPSKMEEMPYDLSRMIINDLAQNNGSI"
     gene            10630..11820
                     /gene="argG"
                     /locus_tag="PKM27_16085"
     CDS             10630..11820
                     /gene="argG"
                     /locus_tag="PKM27_16085"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNR08839.1"
                     /translation="MAPYDKVVLAYSGGLDTSFCIKYLQLEKNLEVHAAIVNTGGFTE
                     EELHTIGTKARQMGAASFTVLDATRDFYQNCLRYLIYGNVLRNNTYPLSVSAERVFQA
                     LSIIEYAKQTGARYIAHGSTGAGNDQIRFDLIFNILGSGLEVITPIRDLKLSRQEEVD
                     YLKKQGVDLSWEKAQYSINKGLWGTSVGGKETLTSDQYLPESAFPTPVTKQEPENLVL
                     TFRKGELTGINQQEYNNPVDAIRALSVIASPFGIGRDIHVGDTIIGIKGRVGFEAAAP
                     VLILKAHHLLEKHTLAKWQQYWKEQLANWYGMMLHEGQYLDPVMRDIEVFLEHSQERV
                     TGSVLLKLFPYRFELHGVDSPHDMMKSKMGQYGEMNNAWTGEDARGFTRILSTANKIH
                     RLAE"
     gene            11900..12865
                     /gene="argC"
                     /locus_tag="PKM27_16090"
     CDS             11900..12865
                     /gene="argC"
                     /locus_tag="PKM27_16090"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNR08840.1"
                     /translation="MVPVAIVGGAGYTGGELIRILIHHPFAELTEVVSESQAGKAVTS
                     VHADLLGETDLIFSKQLEGRPEVVFLCTGHGKAKEFMSKHSLDKKTRVIDLSMDYRLA
                     DPSHDFIYGLPELNRARIRPARHIANCGCFATGIQLALLPLAKEGLLQEDIHVHAITG
                     STGAGQAFSDSLHFSWRSSNVSIYKPFNHQHLPEIRQSLLQLDAGFKAPIHFIPVRGN
                     FTRGIFSTVYTKNDLSAEQALSLYREYYQDEIFVKITEENPDLKQVVNTNKCILHLEK
                     HGPLLLVISALDNLLKGASGQAVQNLNILYGWPEDSGLKLKGMVF"
     gene            complement(13106..>13995)
                     /locus_tag="PKM27_16095"
     CDS             complement(13106..>13995)
                     /locus_tag="PKM27_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08841.1"
                     /translation="QIPGNTSWKFIPKSYSFTDVQNPWGFPTEELLDKMNEQEVRDFI
                     GVKIGDLNSSAIPHSLIGTEVRGFERGLILETEDRLVRKGEELSVTFRSPNFRGISGW
                     QGTLHTGNLLFEGIGNDGKLDLKENNLGHRWAAQGMLTMSWNHHTGMDLDDQSPLFTL
                     IFKAGSNGRLSEMLRIGSQHTVAESYEGKGELGGLSIRFVSPEGNTVTSQSVLYQNYP
                     NPFDQRTVIGINLAQEGRGLLRIMDITGRTVKTIERDWSKGYQEVWLDRREIGATGLL
                     YYRFESRFFTASRKMLIVD"
BASE COUNT         3529 a         3719 c         3338 g         3409 t
ORIGIN      
        1 tagaataatt tattcgccct ttcccgtaac cgaaataatt ttttttgaag tccatcgcaa
       61 gaatttctgt tacaagtatc tcccttttcc caatggcatt tttgcatctt agagtcactt
      121 ttcagtgact catttctatg ttgccatcaa ccgctaactt gctaacttgt taacttacta
      181 acttaccatc ctccttctcc tgatctcctc ctttatgctc tcatatccct gtttcatggc
      241 ccatcgatgg ttcaatctga agaccggacg aagcaggaaa ctcagccagg ccaatccctt
      301 tttccgaaac cggatatccc agtcgaagac gaccaggcag cccttatctt ccggactgaa
      361 ggtccaggtc ccccgaccct ccagatcgcc gaaggcatcg agcactattt ttttacaagg
      421 gatgatctgg atgacttcaa aatcccagcg cagggtatag gggaggtaac ctctggtcag
      481 caaggccact tttttaccca ccccattagc cagcccggga taaatggtat tcacttccag
      541 gtagacggaa ggccaccacc gggacagggc ggatgaatcc ttgatgattt cataaacttc
      601 ttcgacccgg gcgtccagcg cccattgggt gataaaatga aagcgggcca tctttttttt
      661 aatcaaaaat ggaccgggcc tttattcagg gacaagtcca ggatttgttc aaaagatatt
      721 caacgatgag ggaaggcatg gacatccttt tctccggtct ccctttctcc tctaaaaggg
      781 aataacgaat caactaattg atgaggggtg ctttaagcca aataatgttt ttgggcagat
      841 aggcattttc tctcctttcg ccgttcaccc ttcaccgttc acctcccgcg gctgcgggtc
      901 tccttcaacc cgcaaattga tatattactt ctccttccat ctcgcccaag ccattgaatc
      961 cctccagcct ggccatcacg atctcattgg gcgccccggc gtttttatcc acatacactt
     1021 tgatataatt atccgtaaag ccgcaccact gccggtcgtt gccgtcatgc tcaagcagga
     1081 cgggacggga agtgcccagg tgggattgat aaaaaaagtg tttctttttt tcggaaagtt
     1141 gccgaaggat ttcattgcgt tcacggcgga ggtgcaccgg tacggaatgg ggcaaatgga
     1201 ccgccggagt gttctgtctt tcggaatagg taaatacatg caggtaggaa acatccatgc
     1261 ggtgcaggaa ttcataggtt tcccggaaat ggcgctccgt ttcacccgga aagccggtga
     1321 tcacgtcgac cccgatgcag gcatggggca tgagtgattt tatgtactcc acccgggagg
     1381 cgtacaggct tgaaacatat cggcgttgca tcagcctgag caattcatcg ctgccgctct
     1441 ggaggggaat atggaaatgg ggcatgaatc ggcgggagcc ggctacaaat tcaatgatct
     1501 cgtcggtgag gaggttgggc tcgatggagg agatccggat gcggggtaaa ccggcttcct
     1561 catccagggc ccggatcaga tcaatgaaca gggccgattt tttgggcctc atcccttcga
     1621 tcacctcggt accgttgcca aaatcaccca ggttgatgcc cgtgagtacg atttcccgga
     1681 ccccgttagc ggcaatttcc ctggcctttt ccacggcatg ttcgaccgta tcgctccggc
     1741 tccttccgcg ggcaaggggg atggtacaaa acgaacattt ataatcgcag ccatcctgta
     1801 ctttcaggaa ggaacgggta cggtcaccga atgaataaga actgttaaaa gttctaacct
     1861 ccccaatggt gcccgattgc aggggggaga gcccgttgct ctgtgcaagg gcatcgaggt
     1921 actccgtcag cctgaatttt tcgcccgcgc ccagcaccag gctgactccc ggaatgtcga
     1981 cgatttcagc gggcttgagc tgggcataac aacccgtgac gatgactttc gcctgggggg
     2041 aaatattaag cgcctggcgg atcgcgtaac ggcatttttt atcggcttgt tcagttacgc
     2101 tgcaggtatt gatcacatag atatcggccg gttggtcaaa ctcaaggacc tggagccctt
     2161 ccttctcgca aagcgactgg agcgtggagg tttccgcata attgagcttg caccccagtg
     2221 tatgaaaagc gattgacctg ggagcaccca tggcgcaaag ataccgcgaa taaaaaattc
     2281 cgtcccattg tgatattttg gccccaccgg cgaataataa tatatcctga tggatgatcc
     2341 ttcgtgaagg gattgtcggt cagaatttta aacgcacgca attttaacca tctccttatt
     2401 aatcaaacaa ccagtggaaa gaaatgcgcg gaccttcctg gatgttgtgg attcccacaa
     2461 aggaatcatc tacaaaatct cagccgctta ttgccggaat gaaccggacc gcaaggacct
     2521 ggtccaggaa atactgcttc agttatggct ttcattccat aaatacaatg cacaatttgc
     2581 cttgtccacc tggatctacc ggatcgcact caatgtatcc atttctcacc tgaggaccga
     2641 ataccgccgg aaaaggatca accaggacct ccccgctgat ttgctcaacc tgaaggatga
     2701 agaggaaccc cttcaaccgg agtcagatac tgaattttta tttgcctgca tccggcaact
     2761 taaagccata gaccgcgccc taatcctgct ttatctcgag gggaaaagcc agcaggaaat
     2821 cgcggaaatc atcggaacga ccccgaccaa cgtatccacc cgggttttca ggatcaaaca
     2881 gcaactgaaa caaaaattct caaaccacac aaaataaaat catggacgac cacaaactcc
     2941 tggaactctg gaaaaccacc gatcaaaagt tggaagcatg cctgcgcttt aaccggcaaa
     3001 atgcggagga cctcactaaa tggaaagtcc gctccctgct ggcctccatg aatcccctca
     3061 aactttttac cctgcttatg ggcatcctct gggtgtgttt tgtggatctg ctgatcatca
     3121 atacctttcc ctatggcaat ccctttttcc tggtatccgc aggcatccag gttttgctga
     3181 ccaagttcgc gatcggcatc tacctctatc aattgatcct catttaccag gtcgatgtcg
     3241 gggaaccggt gatcaatacc caggaaaaac tggcccgcct gagatcatcc accctctggg
     3301 tcgcccgttt gctttttctg cagttgccgg tatggactac tttttatatc actgcggaca
     3361 tgattaaaga cggaggagcc ggttttttcc tggttcagat ccccgtcacc ttcgcttttt
     3421 tatacctggc gatctggctt tttataaata tcaaatatga aaagcgggat aaaaaatggt
     3481 tcaggctcct cttctcaggc agagaatggg gtccggtaat tcaatccatg gaactcctgg
     3541 accaggtaag ggaatatcgc caaggggcgc ctgaagtgaa ggagtgacac gaagtcaccc
     3601 ttcactcatg gacttgactt ctgtccttct tgctcgcaat tttgcattta taatccccgg
     3661 acatgaaacg cagaaccatt gtggtcatcc tactggtcgt cctcgcaaca gccctggctt
     3721 tcgaccttta ccttcccgtt aaaaaagatt tcaggcagtt tgacccggcg gccgtgggca
     3781 ggctggacgc cgaaatgtgg cgctcctatt acgaaaaaag accggtccgg ttatttttcc
     3841 agctgagccg gctcatgcga acccagtttc acgcccctta tatccggtcc cattttatcg
     3901 cctaccaatc ggccaaagcc gcctttgtct ttaaagaagg gagaaaccgc atccaatacg
     3961 ccggagccct cccttatctc aaaacctatt tcagccagct caatgatctg agcaaggtcc
     4021 cctttaattt ttttaaactg gccgaagaag aactggaatg gtggatcatc cggagagagg
     4081 gagaaaaatt taccccttcc gattgggaag ccgttttagc ccgtgagggg gagatcatgt
     4141 acagcattcc caaagaaaat tttatggatt atgcccgcga ccgggtggcc gccatggtcc
     4201 tccgtgacca aaagggacag tccatcacgg aacaggactg gcaaacgatc acccaaatgt
     4261 gtatcagtgc ctggaccaaa ttccacgccg tggttcagcc ccggatctct tccgctccct
     4321 gaacccggaa accggtaaga atcagttcct tcagaaccaa caagtaaatc aggatacagg
     4381 gccagagttt aaccccatag ggtacaaacc agtttttgcg aatattcgcc ggaaaaagat
     4441 ccgaagcgct gagcacggta aaaataaagg ccaggccgat accggccgtt tgcaggcggg
     4501 tgggatttgc cttggaaaaa taccagatcc ccattccggc cacggccagg ataaacgtgg
     4561 ccgactccga cttatggtta tagatcacca tcgtcaaaaa caggaaggca agcaggttga
     4621 tcctggacaa aacatcagcg aatttccgca atggaaataa ggcgggaagg atcatcccta
     4681 agccgaacac ctgaataaag gttttggact ggaaagccgg ctgcaccaga tggatcaggt
     4741 agagtattga aattcccaat gaagcttcat gatcctggct gaggatgcgc caccaacccc
     4801 ggtattgggc ccacatttca gcgggtggca cccatatcag gggtaaacaa aagagcaccc
     4861 caacagcaag taagccgtga ataataaatc tgaccttgcc cggatagatt aagaaaaaca
     4921 aggccgccag gcccgcgtaa accttgatga atgcggccag ggctatccag actgctgcct
     4981 tccccgtgtt tgatttttca aaatcaataa aagcaaagat cagtaacccg gtcatcaggc
     5041 cgttggactg aaggttttgc aggctggtca gcagttcggg aaaggtaaaa accagcaaca
     5101 aggggatgcg cgccgcccag ggtaccgaga gccgtgtcag agcaaggaaa aataccaaaa
     5161 aattaagcag gttccagaca gtcagtccaa tgcagtcggg caaccaggcc aggattccga
     5221 atgccgccgc aaaacccgga ctgtatttga ataagtcgtg atgctccgcg ggatatagca
     5281 catacatatc cttatggtcc agcagatgaa aaaacgcctg tttgaatatc acataattat
     5341 tataccctgt gtattggagc gggctgcctt caaaggcacg gggcggggcc tggtatttta
     5401 gccaggaggc ataaaccgag accaggccca ataaaacgcc ggcccagagg agcaaggtct
     5461 ttcgttggac ggtgctataa atattcattg agcttgtctt ttcagcacca tgccgggcct
     5521 ggatcccata aatgaccggc catcccaaac ctgaacccca ttgacccaaa cctgttcgat
     5581 gcccgaactg agcgcatagg gatcctcaag gctagcatga tctttgattt gatcccggtc
     5641 catgaccaca aggtccgcat aaaatccggg actgattttt ccgcggaggg ccaggcccat
     5701 atggtctgca ctcagtaggg tcattttatg gatggcggtc tcaaattcaa aagctttaag
     5761 ttgtccgctg aaataatgca ataccctggg aaaactggcc acccctcttg gatggcggcc
     5821 gcccccaaag ccatcgctgc atatattggt atgcttccat tgcatcaacc gggtgatgtc
     5881 gttttcatgc atggacctgc ccatgacgtg ttccggcgca tttttatccc tggcggtttt
     5941 gatcaattgc atataaacct ctgccggctc ttgcttccaa tcctccgcga tctgggcaat
     6001 gcttttaccc acaagatccg gccgggcatc atatctcgat aagatgaggc cgtccggggg
     6061 agtaagctcc cggagtgcaa attcagcggt ttgccggttg tcaaaatccc gtttgggaaa
     6121 atggacctca agggtggaaa gccaaaaatc ataggggtag acatcggctg tgatttgtac
     6181 tcccgccgtt tgggcgctgt ccagccgctg caacaactga tccgccatac cccatttggc
     6241 tttcatggca atcttaaaat gggagatatg taccggcatc cgggttctcc ggccaatatc
     6301 gatgatttca gtcagcgcct cctccaggcc gacgtcctcc gaacggaggt gggaggcata
     6361 tcttccgtcc tttcgggcag ctgccagggc cagtttcaag acttcatctt ttgaggaaaa
     6421 aataccggga tcgtattcca gtccggtgct taatcccagg gctccggatt ttaaatcttg
     6481 tttcagcccc tttttcattt tccggatttc tccatgggtg gcctcccggt taaattcggt
     6541 gttgcccatc gcattaaaac ggagggtgtt gtggccggaa taagatgcaa ggtttaccgg
     6601 aattccctcc cgggtcagtt gttcaaaata atcctgcagg ggaaaacggg aactgccgtc
     6661 ctggccaatg accagggtgg taataccctg attcagaaag ctggaatatt gaccggaatc
     6721 ttcaagattg cggtcgtgat ggctgtgggt atcgatgaaa ccgggggcca ggatctttcg
     6781 gttttggccg tcaatcacct tttctcccgg ccgggggatg agctcaccca ggatagcaat
     6841 tttatggtcc cttatcctta gatcacccag gaataacttt gatccggtgc catccgcgat
     6901 ataaaaattt ttaaacagaa tggtctggcc ggaggataca agacacaaac ccagaaaacc
     6961 aaggcagaga cattcccttt tcaacataac catgaaaata tcggttaatt tcgtgcctgc
     7021 aaggtatggg caggttttat ttgtttatcg cagtttttgg attgctggca tcctgcaatc
     7081 aggaagacgt tgaccagtat aattatacgg tgaggggcag ggtgacccat gaaatcaccg
     7141 ggcttggggt tcccggggtc tgggtgtata ccgaatacgg agagccctgc tgcggatcca
     7201 tcgcattcag aaaggggctg gattccgcaa aaaccaattt tgaaggaaaa tacaccctgc
     7261 agatatcgta ccctgaagac accctgattt accggtttat cactttttta aaagcgggca
     7321 ccctgaacaa ccagttttac cagtattccg gcctggatgt ccaggatatc ggccttaccc
     7381 ggttcgaata caatgagatg gtggctcctg tggtccccgc gaaactgccg gacggtcatc
     7441 ttcaaagctg tgatttcacc atcctcccgg tgggctatgt cgaaattgat ttcaggaaca
     7501 ggggttccgc gccaaccgat accgtttcgg taaaagccat ccggatggac aaaaaccagg
     7561 aatacaaaaa cgtgctgttc tatccttcct attccttcgg tttacatttg tttccggtac
     7621 ccgcttccat ccccaccctg ttgatacagg aaaatacata caatgggaat accgtgaccg
     7681 tgcgggatac cctcaacctc aaggccgggc aacgggtctc gagaaccctg gaaaggtagc
     7741 cttccccatt ccattccagg cactgggatt ggattttaaa ttcaaaagcc gattctatcg
     7801 cttagattag ccggatagat tcaaaagcca tgtctttttt aaaaaacaaa acggtattta
     7861 tcaccggggg cagccggggt attggtaaag ccataggcct tcgaatggcc cgtgaaggcg
     7921 ccaatgtagc gcttgcggcc aaaaccaccg aagagcatcc caaactgcct ggaaccattt
     7981 attccgctgc agctgaaatc gaagcggccg gaggaaaagc cctgcccatc caggcggata
     8041 tccgttttga agaacaggtc gccgcggcca ttcacaaagc ggttgaaacc ttcggcggaa
     8101 tcgatatcct ggtcaacaac gccagtgcca tctccctgac cgatacgccc aacacggaaa
     8161 tgaaacggtt tgacctaatg caccagatca atacccgggg cacttttttg acgactaaat
     8221 atgccctgcc cttcctgaag aaatcggatc atgcccatat cctggtcctc tcccctccca
     8281 tccatcttga tcccaaatgg ctgggcatgc atctcgctta tacgatcagt aagttcagca
     8341 tgagcatgtg tgtcatggga tgggctgaag aattcagggg aaagatcgcc gtcaacgcgc
     8401 tttggccaag gactaccatt gccaccgcag cagtgcaaaa tctccttgga ggggaccaga
     8461 tggttcggct gtcccgcaag ccggaaatcg tggccgatgc cgcttatgaa atattcaggc
     8521 acaatgcctc caccctgtcc ggccggtttt gcattgatga agacatcttg cgggaagccg
     8581 gagtgaatga ttttacttct tacgcagtgg atcccggttc ggaattgatg acggaccttt
     8641 tcttgtgagc ttcgttggag gttgtctaat agagtcgata gggttcgcac ggaggtctgg
     8701 agagcacgga gacaatctta gttttagagg cccttccccg cgcggaaggg ttcgcaagga
     8761 gcgtgggagg gcacggagat atttctttgc ccttattgtg cttgggataa attctaaaaa
     8821 tatacttctc cgtgctcttc gtgttctccg tgagaattct acttgcacgg cgccccccgg
     8881 gcgcccatcc ccgcgcaaat gttcgcacgg agtaagggag ggcacggaga tatttctttt
     8941 gctcatattc gattaactcc gtgctcttcg tgttctccgt gtgcattcta cctgcaaggc
     9001 gcccccaggc gcccttctcc gcgcaaaagt tcgcacggag catgggagga cacggaggct
     9061 ttactttgca cttattgcac ttgggataaa tcttaaaata taattctccg tgcgcttcgt
     9121 gttctccgtg agaattctac ttgcacggcg cccccgtgcg cccatccccg gtaaaaaaaa
     9181 agttcgcacg gagcaaggga ggacacggag atatttcttt acctcccatt caatgattct
     9241 ccgcgttctt cgtgttctcc gtgagaacta cttcgtgcga attccactcg ttctgctccc
     9301 tcgtgcggaa ggctcagcct cccaaagaca atcacattag ccgtaccgcc ggccactctc
     9361 cccgtggtcc tgtaccggaa ttcaatccgg tcaatccggc gctctcctcc cggcagatca
     9421 atgatccggc tttcgcctcc tttgcggata tcctgacgaa tggccacatc aaaccgttcg
     9481 ccattttcaa aaaccacatc gaaattgctg atgtgcaggg gggcgtcgac gacttttatt
     9541 ttgagtttgg tgtaccagtc attgccgccg actttaatga cgtcgtgatc ggaggagaat
     9601 ttcaccgctc gttccccaat catcttccag gcaggctgga cggtgaaaga agtcaaaatg
     9661 aacaagacag cagcaagata aaaaatggtt gtacgcatat ctgagttttt aaagtttcta
     9721 acgggtaaga ctaaatttag cccatccggg tttaaaccgg ctaaaaatat tcaaacattt
     9781 ttatctgcga gttccggcta atcgataact ttgcaccagt gatacctcag gtactttcac
     9841 atatagaatc tttcatcagt caggacctcc tttcccccag gtcctttcaa cctgcccgtt
     9901 gatcctccaa cgaaggtatt tatcattttc ttaaataaaa taattaaccg agttggattt
     9961 attcgaacaa attccatgtt aacatagcca ttgtagtaaa aatgaaccat gaaaatcaaa
    10021 tcaccgatgc actggcggta caagaagcac cagtgacagt agtcgtagcc gggccggaac
    10081 acgcccgtta tgccgaggag atttgccacc tgatcgaaga atctgccaaa gccaggaaaa
    10141 ccgggatcgc aaaacgctct ccggaataca ttatcaaaaa actcaacagc ggggacgccg
    10201 ttattgccct gcaaggagaa aaactggcgg gtttttgtta tatcgagacc tggagtcacg
    10261 gccaatacgt agccaattca ggactgatcg tcgccccgga cctgcgcaag gcagggcttg
    10321 gccgggagat caagatcaaa gcctttaacc tggcccggga taaatacccc tatgccaagg
    10381 tcttcgggat caccaccagc ctggcggtga tgaagatcaa taccgaactc ggttataaac
    10441 cggttacttt ttccgaactg acccaggatg aagccttctg gaaaggctgc agcagttgtc
    10501 ccaactacga tatcctgcaa cgcaacgaaa gaaaaatgtg cctatgcacc ggcatgctgg
    10561 ccccctctaa aatggaagaa atgccctatg atctttcccg gatgatcatc aacgatttag
    10621 cacaaaacaa tggctccata tgacaaagta gtcctcgctt acagcggcgg actggatacc
    10681 tccttttgca tcaaatacct gcagctggag aaaaacctgg aagtacatgc ggcgatcgtt
    10741 aataccgggg ggttcaccga agaagaactt cacacgatcg gaaccaaagc gcggcaaatg
    10801 ggggcggctt ccttcaccgt gctggacgca acccgtgatt tttaccaaaa ctgcctccgg
    10861 tacctgattt acggaaatgt tctgcgcaac aatacctacc cactatcggt cagtgcggaa
    10921 agggtgtttc aggccctctc catcatcgaa tacgccaaac aaaccggtgc ccggtacatc
    10981 gctcacggga gtaccggcgc gggcaacgac cagatccgtt ttgatcttat ttttaatatc
    11041 ctgggctccg gactggaggt gatcaccccc atccgggatc tgaaattgtc ccgtcaggaa
    11101 gaagtcgatt acctgaaaaa acaaggagtg gacctttcct gggaaaaagc acaatattcc
    11161 atcaacaaag ggctctgggg taccagcgtg ggaggtaagg aaaccctgac ttccgatcaa
    11221 tatctccccg aatccgcctt cccgactccc gtcactaaac aggaaccgga aaacctcgtc
    11281 ctcactttca ggaaaggaga acttaccggc atcaatcaac aggaatacaa caatccggtg
    11341 gatgccatcc gggctttatc cgtgatcgct tccccttttg gcataggcag ggacattcat
    11401 gtaggggata ccatcatcgg catcaaaggc cgcgtcggtt ttgaggccgc cgccccggtc
    11461 ctcatcctca aagcccatca tttgctggaa aaacataccc tggcgaaatg gcaacaatac
    11521 tggaaagaac aactggccaa ctggtacggc atgatgctgc atgaaggcca atacctggac
    11581 ccggtcatgc gggacatcga agtcttcctg gaacacagcc aggaaagggt gaccggctcg
    11641 gtgcttctca aactcttccc ctaccgcttt gaattgcacg gggtggattc tccccacgat
    11701 atgatgaaaa gcaagatggg acaatacgga gaaatgaaca atgcctggac cggcgaggat
    11761 gcccggggct tcacccggat cctttctacg gccaataaga tacaccgttt ggcggagtag
    11821 cccctcagcc ttaacctcaa cttcagcctc agcctcagcc ttaacctcaa cctcaattta
    11881 tcccttattt ttgcctccta tggttccagt ggcgattgtt ggcggagccg gttataccgg
    11941 tggagaattg atccggatat tgatccatca tccgttcgcg gaactcaccg aggtggtgag
    12001 cgaaagccag gccggtaaag cggtgacctc ggtgcatgcg gatcttttag gggagaccga
    12061 cctgatattt tccaaacaat tggaaggcag gcccgaggtg gtttttttat gcaccgggca
    12121 tggaaaagca aaagaattca tgtccaagca ttccctggac aaaaaaacga gggtgattga
    12181 cctgagtatg gattaccggc tggccgatcc ttcccacgat tttatatatg gattgcccga
    12241 attgaaccgg gccaggatca gacccgcgcg ccacatcgcc aattgcggct gctttgcaac
    12301 gggtatccaa cttgccttgc ttcccctagc caaagaagga ttgctccagg aggatatcca
    12361 tgtccatgcc attacgggca gcacgggggc aggtcaggcc ttcagcgaca gccttcattt
    12421 cagctggcgc agttcgaatg tcagtattta caaacctttc aaccaccagc acctccccga
    12481 gatcaggcaa tccctgctcc aactggatgc cgggttcaaa gcacccatcc attttatacc
    12541 ggtccgggga aattttaccc gcggtatttt ttccacagtg tacacaaaaa atgacctcag
    12601 cgcggaacaa gccctatccc tctaccggga atattaccag gatgaaattt ttgtaaagat
    12661 cacggaagaa aatcctgacc tgaagcaggt ggtcaatacc aataaatgca tcctccacct
    12721 ggaaaaacac gggcccctcc tcctggtgat ttccgctttg gataatttat tgaagggcgc
    12781 cagcggccag gccgtgcaga atctcaatat cctgtatggc tggccggaag acagcggact
    12841 gaaattaaag gggatggttt tttagacagg ccttacccat tccaccgatc gggactccct
    12901 acttcccttt gttctttttt tcagcccttt tttctttcag ggtcttcgtt gctttctttt
    12961 taacctcttt tttctggtct ttgctttttg acataacgaa caaatttatc aatcctaaag
    13021 gtaaataata ttataaatta ttcatatata aaaaaagccc cggatcgctc cggggccttc
    13081 cattttattc attctgggcc ttggttcagt ccacaatgag cattttcctg ctggcggtga
    13141 aaaatctgga ttcaaagcgg tagtacagta agcccgtggc gccgatctcc cggcgatcca
    13201 gccatacttc ctgatacccc ttgctccaat cacgctcgat cgttttgacg gtacggccgg
    13261 taatatccat gattcttagg agtcccctgc cctcttgggc aaggttaatc ccgatgactg
    13321 tacgctgatc aaacggattg gggtagtttt gatacaatac actttgtgag gtcaccgtat
    13381 tgccttccgg gcttacaaat cgaatcgaca ggccacccag ttcacctttc ccctcatagc
    13441 tctccgccac ggtgtgttgg gagccaatcc gcaacatctc tgacaagcgg ccgtttgacc
    13501 cagccttgaa aatcagggta aacagcgggc tttggtcatc caggtccatt cccgtgtgat
    13561 gattccaact catggtcagc atcccttgtg ccgcccagcg atgcccgaga ttgttttctt
    13621 taagatccaa ttttccatca ttaccaattc cttcaaacag gaggttgcct gtatgcaggg
    13681 taccctgcca gccgctgatt ccccggaagt ttggtgaccg gaaggtcaca ctcaattctt
    13741 ctccttttcg aaccagccgg tcttcagttt ccaggatgag gcctctttca aagccccgga
    13801 cctcagtgcc gatcaagctg tgcggtatag cactgctgtt caggtctccg atcttcacac
    13861 ctataaagtc cctcacctcc tgttcattca tcttatccag cagttcttct gttggaaaac
    13921 cccagggatt ctgaacatcg gtaaaggaat acgatttcgg tataaatttc cagctcgtgt
    13981 tgcccggtat ctggt
//
LOCUS       DAOQXT010000113        13955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4855_length_13955_cov_5.676475, whole genome shotgun sequence.
ACCESSION   DAOQXT010000113 DAOQXT010000000
VERSION     DAOQXT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13955
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4855_length_13955_cov_5.676475"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..772)
                     /locus_tag="PKM27_16100"
     CDS             complement(53..772)
                     /locus_tag="PKM27_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08842.1"
                     /translation="MIRIAIAVLIFLFSTGWSAAQGTRFGLKAGPSVGFQKWNYFQNG
                     PLYKYHGALWIESYQEEDPFSIFAQAGYHVKGSATRFYGPIQIDNQIYDIPANEFIFN
                     NLSLQAGVKKKFLWSKLSGYYAFGLRGEYTLSTNLDSYDRINRLVPVYPFNSAVKRVL
                     AGFTLGGGLEIPFGEFAGGILELSISPDLTRQYFQPQINNIIDIFQPGRTTSIPEKSI
                     KNLTLELSFGVYLTRKVIYVD"
     gene            complement(769..1842)
                     /locus_tag="PKM27_16105"
     CDS             complement(769..1842)
                     /locus_tag="PKM27_16105"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR08843.1"
                     /translation="MARLRILIGALNWGLGHATRCQPVIEECLRRGHEVHLAADGLAA
                     MVFEKNNPDLPVHRLAPLNIKYSTWFWLKLLAQTKQLLTWFWKDRRETRRLQRLYQYD
                     LILSDSRPACRVQGVPGILIINQPSPWIPGRLIRWFTHRTLWAIINRFDEICIPDLPR
                     ENNLSGKLIHLPGRIHRTFVGLLSRMQAPSSGASFQIPGRILAILSGPEPSRTNFETE
                     LILRLAGHDYRIAGGRPDLKTRTDRYLSYMDAVGLADEISKAEYIICRSGYSSLMDLA
                     PFKKKLILVPTPGQTEQEYLAGRLQSKRQAVVWNMKSESLQEVLNSAGLSSPFYLENE
                     PGLLGSAIRKWEEYCQKKSNLQG"
     gene            complement(1855..2469)
                     /locus_tag="PKM27_16110"
     CDS             complement(1855..2469)
                     /locus_tag="PKM27_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08844.1"
                     /translation="MKYWILALALVVGTGCAKVYYSPDAKSLASRHKRIAIIPPKVSI
                     TPRKKSDLEALKEQQKLESVNFHQEIYSWLLRRKMQNKLRVDVLDLETTIAKLKEAGY
                     PNERSLTPVELQDLLGVDGILTSNFSLSKPMSEGGAIALGLIFGVWGATNEVVVGMEI
                     HDRPKEKVIWSFNHKLSGSTFSTPARLVDQLMRQASKKMPYISK"
     gene            complement(2518..4068)
                     /locus_tag="PKM27_16115"
     CDS             complement(2518..4068)
                     /locus_tag="PKM27_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNR08845.1"
                     /translation="MERELIINSTPRDVEIALLEDGKLVELHHQQSDENFQVGDVFLG
                     TVTKLMPGLNAAFVNIGHSKDAFLHYTDLGPPLMSFLQFAEKSIQGKLQTHLLDQFPI
                     EPEINKNGKINQVLARKQNILVQILKEPISTKGPRLTGEITIPGRFMVLTPFIDQVAV
                     SKKIDDQEERKRLQILVESIKPKNFGVIVRTAAAGKKVADLHEEITSLAQKWETIHKQ
                     LFKARAPMKLLSELDKTSSMLRDLLNASFNRIITNDRELYQNTRTLLQNISPEQTKIV
                     QHYKGDRAIMDQYGVSRQIKSSFGKTATMNSGAYLVIEHTEAMHVIDVNSGPKMQKSD
                     QQSAALAVNLEAAAEIARQLRLRDIGGLIIIDFIDMKSQDHKNHLFRMMREYMASDRA
                     QHSILPLSKFGLMQITRERVKPAVNINTSEVCPTCGGSGQIQPSILVTDEIQRDLEFI
                     VENRPNTRITLEVHPFLAAYLTKGIWSRQVKWWWTYKRWFSVRANENIGLTKYKLFDG
                     NEDEIRMN"
     gene            complement(4379..5161)
                     /locus_tag="PKM27_16120"
     CDS             complement(4379..5161)
                     /locus_tag="PKM27_16120"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR08846.1"
                     /translation="MKPSVLFAGSVILFFVLFFGFSKIAPEVRNPEKSGSIHFDNKDL
                     TVELNSLKDSLSEGDKAYLAGLESELASASQDSSRIQSLQKISSFWYQQGRIGFSAEY
                     AVKIAQIFPTGESWSIAAANLALAAKTPGLAEEKISDYITRSKAAFIQAGEADPGNIS
                     HKLNYALLATEYPSADNPMEGILTLRKLNEDFPEDLTVLYHLARLAIQTGQWDRAKER
                     LETLFKLDPGNARAACLMVQISQHDGEVAKAAEWQKKCDSNQ"
     gene            complement(5158..5481)
                     /locus_tag="PKM27_16125"
     CDS             complement(5158..5481)
                     /locus_tag="PKM27_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNR08847.1"
                     /translation="MRKADLVAMISEKTGVPKVDVLVSLEMFFKEVKNTLAEGENVYI
                     RGFGSFIIKKRARKIGRHIKKNVAIEIPEHFIPSFKPAKVFVEQVKNKVTQMPEGEVE
                     GEEEE"
     gene            5691..7001
                     /locus_tag="PKM27_16130"
     CDS             5691..7001
                     /locus_tag="PKM27_16130"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR08848.1"
                     /translation="MIRAVFIAAGFFLSLETIAQTASPTKARMPVLTPINDPALAGMS
                     VQRIERLDKALETMIREQKLPGLVALVARRGKIVLHKAYGLADAPANRSLHKDDIFRI
                     ASMTKAITATAVMMLYEEGGFSLDDPIAKWIPAFKNPQVLKAFSPADSSYTSRPASRE
                     ITIRHLLTHTSGIGYGMIDGDERVKKIYKKAGIIELYSTDPVTTEDNIKRLAALPLHH
                     DPGEKFTYGMGLDVLGYFIEILSKQSFPDFLASRLFQPLGMKDTYFYLPDDKAGRLVK
                     IHLPVKAGTWSTDPDEGYDEDYPIKGARSNCSGGAGLSSTAIDYAIFLQMLLNDGVYN
                     GKRFLSRPTVSLLTRSNQIGDLWGGEEGRDHFSLAFSVLNKKGSELGIGSEGRFSWGG
                     YFNTNYWVDPKEQIVAVLMKQTRGLPEDPSEAVFTRLVYQSIDD"
     gene            complement(7165..8553)
                     /gene="fumC"
                     /locus_tag="PKM27_16135"
     CDS             complement(7165..8553)
                     /gene="fumC"
                     /locus_tag="PKM27_16135"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNR08849.1"
                     /translation="MDFRKEKDSLGYVDVPANAYWAAQTQRSTQNFKIGGQRIPIEII
                     RAFAILKKAAAITNHKLKVLPKEKMMLISRVCDEILEGKLDDQFPLVVWQTGSGTQSN
                     MNVNEVIANRAHLLSGGSLTDTHKVLHPNDDVNKSQSSNDTFPTAMHIAAYSAIMEWT
                     LPGMTELRNALSKKARAFMKVVKIGRTHLMDATPLTLGQELSGYVAMLDHGIKCVSHS
                     LAHLSELALGGTAVGTGLNTPKGYAKKVASEIARISGYPFVTAPNKFEALATHDAMVE
                     AHGALKTVAASLFKIANDIRLLASGPRSGIGEIIIPDNEPGSSIMPGKVNPTQSEAMT
                     MAMAQVMGNDVAINIGGMNGHFELNVFKPLIAYNFLISARLIGDACNSFTEHCVLGLE
                     PNHARIKHNLDNSLMLVTALNTRIGYDKSAEIAKKAHKEHSTLKEAAVALGYVTAEQF
                     DQWVRAEDMVGQ"
     gene            8710..9306
                     /locus_tag="PKM27_16140"
     CDS             8710..9306
                     /locus_tag="PKM27_16140"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNR08850.1"
                     /translation="MAFTLPALPYSFDALEPHIDTRTMEIHHGKHHQAYVTNLNTAIE
                     NTEWADKSIEEILANVSRLSPAVRNNGGGHYNHSLFWQIMGPGKGGVPTGIIGDAIQG
                     SFGSFDAFKEALSKAGMGRFGSGWAWLSKDAQGKLFISSTPNQDNPLMDLAEQKGTPI
                     LGVDVWEHAYYLHYQNRRADYLNAFFNVINWDAVAARL"
     gene            complement(9676..9918)
                     /locus_tag="PKM27_16145"
     CDS             complement(9676..9918)
                     /locus_tag="PKM27_16145"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNR08851.1"
                     /translation="MHYAYILRSELSGKYYYGSSGDIQKRLADHNKGRVKYTKPFRPW
                     KLHYWEEFQTNSEALRRENFFKTIDGYRWLKSMNIT"
     gene            complement(10303..11088)
                     /locus_tag="PKM27_16150"
     CDS             complement(10303..11088)
                     /locus_tag="PKM27_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08852.1"
                     /translation="MRIHIYIGISLFLAVGMACKNETPVQTGQNINGAFNVEGSDPEA
                     IWVAEDAIAASGGAEAWADTRYIKWNFFGRRWLLWDKKDGKVRIENTGNDTKLILDLN
                     TRQGKVFKDGREWEDPDTLKKYLDNAYRIWINDSYWLAMPFKMKDPGVHLRYVREDTT
                     VKGIASDVISLTFDSVGVTPQNKYEVWVGKNSSLVAQWAYYPNVLDSTPSIINPWDGY
                     VKYGNIFLSSDRGENRQITGIKVYESLPASLFESFEAVDFERL"
     gene            11216..12562
                     /gene="ffh"
                     /locus_tag="PKM27_16155"
     CDS             11216..12562
                     /gene="ffh"
                     /locus_tag="PKM27_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNR08853.1"
                     /translation="MFESIHDNLDNAFKFLRGENKLTELNIAEAIKEIRRALVGADVN
                     YKIAKEFTDKVKDKALGTENVLKAVKPGELMIKIVFDELTELMGGQSAGINLKGEPGI
                     VLVAGLQGSGKTTFSAKLANYLKTKKGKKPLLVACDVYRPAAIDQLSTLGEQINVAVF
                     KEPDNKNPLEIAHKALAFARQNHHDVVIVDTAGRTTLDDAMMTEIREIKEAIHPTESL
                     FVVDSMIGQDAVTTAAAFHEAINYDGVVLTKLDGDTRGGAALSIKYTVGKPIKFISMG
                     EKLDTLDVFYPDRMAQRILGMGDIVSLVEKAQEQFDEKEAKALEKKIRKNKFDFNDFL
                     SQINQIKKMGDLKNLVSMMPGMGKALKDVDINDSAFKKVESIIQSMTPTERANPELLN
                     MNRKNRIARGCGQSVHEITAFIKQFDQMKQMMQQLSTGKGMGTMMKQMQNMRGFQG"
     gene            12597..>13955
                     /locus_tag="PKM27_16160"
     CDS             12597..>13955
                     /locus_tag="PKM27_16160"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNR08854.1"
                     /translation="MKTISSLLWSGLLILLTACQPKTASRVATAPPSQVSQSTPTPAQ
                     PVTPALEAPSEVGIDLGAKIPLDPSVRIGVLPNGMTYYIKKNAKPEQRVELRLAVKAG
                     SILEDEDQLGLAHFVEHMAFNGSKNFKKQELVDYLESVGTRFGPDLNAYTSFDETVYM
                     LQARSDEADKLEKGLLVMEDWASGVAFDPVEIDKERGVVVSEWRTSLSGEQRIMQKVL
                     PVILYQSHYANRLPIGKPEIIEKASYEAVKRFYKDWYRPELMAVMAIGDLDPDKMETE
                     LRNRFSKIPASPASARKREHIEVPMHPETKVIVATDKEAAFTQAQVYYKHTKKTPASI
                     QDYRNGLLANLYNGMLNVRLDELAQKPNPPFNYAGAGFGDFLANLNAYSSSVSTPEGQ
                     ALTGLQAVMIENERAFRHGFTSTEFERQKEDLMRRMESAVKEKDKTESGAIASTLVGH
                     FLE"
BASE COUNT         3471 a         3495 c         3435 g         3554 t
ORIGIN      
        1 tgactactga ctactgacta ctgactactg actactgact actgactact gactaatcca
       61 cataaataac cttcctcgtc aaatacaccc caaacgaaag ctcaagcgtc aggtttttaa
      121 tgcttttttc cggaatggaa gtagtgcgcc ccggctggaa gatatcgatg atgttgttga
      181 tctggggctg aaaatactgg cgggtcaggt cgggactgat gctgagttcg aggattccgc
      241 cggcaaactc tccaaacggg atttccaggc ccccgcccag agtaaatccg gcaagcactc
      301 tttttacagc ggaattgaag ggatagaccg ggaccagccg gttgatgcgg tcgtaggaat
      361 ccaggttcgt agaaagagtg tattccccgc ggagcccgaa ggcataataa ccggaaagtt
      421 tgctccacag gaattttttc ttaacccccg cctggagcga aagattgtta aagataaact
      481 cattggcagg gatatcgtag atctggttgt caatctggat gggcccataa aagcgggtgg
      541 cgctcccttt gacatgataa cccgcctggg cgaagatcga aaaaggatcc tcctcctggt
      601 acgattcgat ccacaaggcg ccatggtatt tatacagcgg accattctga aaataattcc
      661 acttttggaa gcccaccgaa ggccctgctt tcaatccaaa gcgggtaccc tgggcagcac
      721 tccatccagt agaaaaaaga aaaatcaaaa cggcaatcgc gatccggatc atccctgcaa
      781 attagatttt ttttggcagt attcttccca cttccggata gctgaaccca gcagacccgg
      841 ttcgttttca agataaaacg gggaagacag tccggcacta ttcagcacct cctgcaggga
      901 ttcggacttc atattccaga ccacagcctg ccttttggat tgcagccttc cggccagata
      961 ttcctgttcc gtctggccgg gggtgggtac gaggatcagt ttctttttga atggggcaag
     1021 gtccatcagg ctgctgtaac cagacctgca gatgatgtat tccgctttgg agatttcatc
     1081 agccaggccg accgcgtcca tataagaaag ataccggtcc gtccgggtct ttagatcagg
     1141 cctgcccccg gcaatccgat aatcatgacc agccaaccga aggatcagtt ccgtttcaaa
     1201 gttcgtgcga gaaggctccg gaccagaaag tatggcaagt atccttccgg gtatttgaaa
     1261 gctggcaccg gaagacgggg cttgcatccg gcttaaaagt cctacaaaag tcctgtgaat
     1321 tcgcccgggc aggtggatca gtttcccgct gaggttattt tcccgcggga ggtccgggat
     1381 acagatttca tcgaaccgat ttatgatcgc ccacaaagtc ctgtgggtga accaccggat
     1441 caaacggccc gggatccatg gagaaggctg attgatgatt aaaattcccg gcacaccctg
     1501 aaccctgcag gccggcctcg aatccgagag gatcaggtca tactgataca atctttgcag
     1561 gcgccgggtt tccctgcggt ctttccaaaa ccaggttagg agctgtttgg tttgggccag
     1621 gagctttagc caaaaccagg tgctatattt aatgttgaga ggagccagcc ggtgcaccgg
     1681 cagatcagga ttatttttct caaaaaccat ggcggccaaa ccatcggcag ccaagtgaac
     1741 ttcatgacct cttctcaggc attcctcaat caccggctgg caacgggtgg catgccccaa
     1801 gccccagttg agcgccccga tgaggatgcg aagcctggcc attcccaagg ccgtctattt
     1861 ggatatatag ggcatttttt tgctggcctg gcgcatcagc tggtcaacca gcctggccgg
     1921 ggtactgaac gtgctgccgg agagtttatg gttaaaactc caaatgacct tttctttggg
     1981 gcggtcatga atttccatgc cgaccaccac ctcattggtg gctccccaaa ccccgaagat
     2041 caggcccagg gcaatcgcac ctccttccga catgggcttg gacaaagaaa aattggaagt
     2101 caggattccg tcgactccca acaaatcctg cagttcgacc ggggtcaacg acctttcatt
     2161 tggatacccc gcttccttaa gcttggctat ggtcgtttca agatccagga cgtccaccct
     2221 cagtttgttt tgcattttgc ggcgaagcag ccaggagtat atttcctggt gaaaattgac
     2281 cgactccagt ttctgttgtt ctttgagggc ttccaggtct gattttttcc ttggcgtgat
     2341 ggacactttc ggtggaatta ttgcaatgcg tttatgccgg ctggccaggg atttggcatc
     2401 cggactgtaa tacactttcg cacagcccgt acctaccacc aaagccaggg ccaaaatcca
     2461 atacttcata tatataaaaa attatagatt atcatcgact attacccgcg gcatgtttca
     2521 gttcatccgg atctcatcct cattcccgtc aaacaatttg tatttggtga gcccgatgtt
     2581 ctcgttggcg cgaaccgaaa accaccgttt ataggtccac caccatttga cctgccggct
     2641 ccagatccct ttcgtcaggt aagccgccag gaaaggatgc acttccaggg tgatgcgtgt
     2701 atttgggcgg ttttccacta taaattcgag gtcccgctga atttcatcgg tcacgaggat
     2761 agagggctgg atttggccgg accctccaca ggtgggacaa acttcgctcg tattgatatt
     2821 caccgcgggc ttgaccctct ccctggtgat ttgcatgaga ccaaacttcg agagtgggag
     2881 aatgctgtgt tgggcgcggt ctgaagccat atattccctc atcatcctga aaagatgatt
     2941 tttatggtcc tggctcttca tgtcgataaa atcgatgatg atcaggcccc cgatatcacg
     3001 gaggcgcagt tgccgcgcga tttctgcggc ggcttccagg ttcacggcga gggcagcgga
     3061 ctgctgatcc gatttttgca ttttagggcc gctgttgacg tcgatcacat gcatggcctc
     3121 ggtatgttca atcaccaggt aggctccgct gttcatcgtg gcggttttgc cgaatgagga
     3181 tttgatctgc ctggatactc cgtattggtc catgatcgcc cggtccccct tgtaatgctg
     3241 gacgattttg gtttgttccg gggatatgtt ttgcagcagg gtgcgcgtgt tctggtacag
     3301 ttcccggtcg ttggtgatga tccggttgaa cgatgcgttg agcaggtccc ggagcatgga
     3361 ggaggtctta tccagctcgc tgaggagttt catcggggct ctggccttaa acaattgttt
     3421 atgaatggtt tcccattttt gggccaggct ggtgatttct tcatgcaagt cagccacttt
     3481 tttaccagcg gcagcagtac ggacgatgac cccgaagttt ttaggcttga tggattccac
     3541 gaggatctga agccttttac gttcttcctg gtcatcgatt tttttggaaa cggccacctg
     3601 atcaatgaat ggggtcagca ccataaaacg cccgggtatg gtgatttccc cggtgagacg
     3661 ggggcccttg gtggaaatgg gttccttaag gatctggacc aggatgtttt gtttccgggc
     3721 gagaacctgg ttgattttgc cgtttttatt gatttccggt tcgatcggaa actggtccag
     3781 caggtgggtc tggagtttac cctggatgga tttttcggca aactgcagaa aagacatcag
     3841 gggcggcccc aggtcagtat aatgtaggaa ggcatctttg gaatgcccga tattgacaaa
     3901 ggcagcattc aatccgggca tcagtttggt caccgtgcct agaaaaacgt ctcccacctg
     3961 gaaattttcg tcagactgct gatgatgcaa ctcgaccagt ttgccatcct caagcagggc
     4021 aatttctacg tccctcggag tagaattgat aatgagttcg cgctccattt acacttgttt
     4081 gaaactaggc ttcaatgaac agaaatccgg gttttcccgg ttggagcgaa taacaaaaag
     4141 atgaaaattg ttgagagggt ttgcaccctc tctccgtcca taaaagccta agaaatcact
     4201 atctcagttt ctttttatgt ctgtttttac gtaatctttt cttacgctta tgggtggcca
     4261 ttttatggcg ttttcttttt ttaccgcaag gcatgtttaa aaattaattt actttttttg
     4321 ttaataatat ggttatccgg caaggtgacc cttagggtac cttaacgttt gatgtcaatc
     4381 attggttcga atcacatttt ttttgccatt ccgctgcttt agccacctct ccatcgtgtt
     4441 gggaaatctg gaccatcagg caggcggccc tggcatttcc cgggtccagc ttaaacaagg
     4501 tttccagtct ctctttggcc cggtcccatt gtccggtctg tatcgccagg cgcgccaggt
     4561 gatacaatac cgtcaggtct tccggaaaat cttcattgag ttttctcagg gtcagaatgc
     4621 cttccatcgg gttgtcagcg gaggggtatt cggtggccag gagtgcataa ttcaacttat
     4681 ggctgatatt accgggatcg gcttcgccgg cctggataaa agccgctttg gacctggtga
     4741 tataatccga tattttttct tccgcaaggc cgggagtttt ggcagccagg gcaagattgg
     4801 cggcggctat ggaccaggat tctccggtcg gaaaaatttg ggcaattttt acagcatatt
     4861 ccgcagaaaa gcctatcctt ccctgctgat accaaaagga cgatattttt tgaaggctct
     4921 ggatcctgga agaatcctgc gaggcggaag ccaattccga ttcgagtccg gccaggtagg
     4981 ccttatcccc ttcggaaagg ctgtccttca ggctgtttaa ttcaacggtc agatctttat
     5041 tgtcaaaatg tatggatcct gacttttcag gatttcgaac ttcgggagct atcttactga
     5101 atccaaaaaa caatacaaaa aacaaaatca cggatcctgc aaagaggacg gaaggtttca
     5161 ttcttcttct tctccttcta cttctccttc aggcatttgg gtgactttat tctttacctg
     5221 ttcgacaaaa acctttgccg gtttgaaaga agggatgaaa tgttcaggaa tttcaatggc
     5281 cacattcttt ttgatatgcc tgccaatttt ccgggcgcgt tttttaataa tgaacgaacc
     5341 aaatcccctg atatatacat tttctccttc agccaaggta ttcttaacct ccttaaaaaa
     5401 catttccaga ctcaccaata catccacctt cggcacaccg gttttttctg agatcatggc
     5461 gacaagatca gcttttctca tcagtaattg atttttaatt gattgtaaaa caatggtccg
     5521 gtttcttatg gaaccgcgtg caaagttctg attttatcct gaattgacga aatattttat
     5581 aaaatattta ttttctgtta gttagaccat atttccaggg ccttaacagt tgataccggt
     5641 tttgcagcca aaaccttata atgttatctt tcggagaaat ggtaaaagaa atgatcagag
     5701 ctgtttttat tgcggcagga ttcttcctga gccttgagac gatagcacag acagccagtc
     5761 cgaccaaggc caggatgccg gttttaacac ctataaatga tccggccctg gcagggatgt
     5821 ctgttcagcg gattgagcga ctggataaag ccttggaaac catgatccgg gaacaaaaac
     5881 tcccgggatt ggtcgccctg gtagccagaa ggggtaaaat tgttttgcat aaggcctatg
     5941 gtctggcaga cgctccggca aaccgaagcc ttcataagga tgacattttt cgcattgcat
     6001 ccatgaccaa ggccattact gccactgcgg tcatgatgct atatgaggag ggcggattct
     6061 ccctggacga tcccattgcc aaatggatac ctgcctttaa aaacccccag gtacttaagg
     6121 ccttcagtcc ggctgattca agctatacct cccggccggc cagcagggaa atcaccatcc
     6181 gtcatcttct gacccatact tcaggcatcg ggtatggcat gattgacggg gatgaacgag
     6241 ttaaaaaaat atataaaaaa gcggggatca tcgagttata ctccaccgat ccggtcacca
     6301 ctgaggacaa tattaaacgc cttgcggctc tccccctgca tcatgatccg ggtgaaaaat
     6361 tcacctatgg catggggctg gacgtactcg gttatttcat tgaaatactg tccaagcagt
     6421 cgttccctga tttcctggcc agccgtctct ttcagccgct cggcatgaag gacacctatt
     6481 tttacctgcc ggatgacaaa gccggccgcc tggtgaaaat acatctccct gtcaaagctg
     6541 ggacctggtc cacggatccg gacgaagggt atgatgaaga ttatcccata aagggcgccc
     6601 gatccaactg ttccggagga gcgggactca gttcaacggc catagactat gccatttttc
     6661 ttcaaatgct gctgaacgac ggagtgtaca acgggaagcg gttcctgagt cgcccgacgg
     6721 tcagcctgct cacccggtca aaccagatcg gcgatctctg gggcggggaa gaaggcaggg
     6781 atcattttag cctcgctttc agcgtgctca ataaaaaagg ctccgaactt ggaatcggtt
     6841 ccgaaggacg ctttagctgg ggcgggtatt ttaacaccaa ctactgggtg gatcccaaag
     6901 aacaaatcgt agccgtcctg atgaaacaaa cgcggggcct cccggaggat ccgtcggaag
     6961 cggtgtttac cagattggtt taccagagca ttgatgatta gggattgtga acattcagct
     7021 atcagcattc agcattcagc attcagcatt cagcaatcag caatcagcta tcagcattcg
     7081 gcaatcagct atcggctgtc cgcgtttcgc tcccttcgcc cttaaccatt cgcccttaac
     7141 cattcgccct tcaccattgt caactcattg cccgaccata tcctccgccc tcacccattg
     7201 atcaaactgc tccgcggtga catatcccag ggcgacggct gcctctttga gggtgctgtg
     7261 ctccttgtgc gcttttttgg cgatctcagc ggacttgtca tacccgatcc gggtattcag
     7321 ggcggtcaca agcatcaggg aattgtccag gttatgtttg atccgggcat gattcggttc
     7381 taggcccagg acacaatgct ctgtaaaact gttgcaggcg tcgccgatca gtctggctga
     7441 gatcaggaaa ttatacgcga tcaggggctt aaatacattg agctcaaaat gcccgttcat
     7501 ccctccgata ttgattgcca cgtcatttcc catcacctgg gccatggcca tggtcattgc
     7561 ctcagattgg gtcggattca ctttcccggg cataatggaa gaacccggct cgttatcggg
     7621 gatgatgatt tcaccgatcc cgctcctcgg gcccgaggcc aggagcctga tatcattagc
     7681 gattttaaac agggaggcgg ctacggtttt taaagcaccg tgggcttcca ccatggcatc
     7741 gtgggtggcc agggcctcga atttattggg tgcggtcaca aagggatatc ccgaaatccg
     7801 ggcaatttca cttgcgactt tttttgcata gcctttggga gtattgaggc cggtgcccac
     7861 cgctgttcct ccaagggcca gttcagacag atgggccagg ctgtggctga cacattttat
     7921 tccatggtcg agcatggcga catagccgct taactcctgg cctaaggtca gcggggtggc
     7981 atccatgagg tgggtgcgcc cgattttaac caccttcata aatgccctgg cctttttgct
     8041 tagcgcattt ctgagttcgg tcatgccggg aagggtccat tccatgatgg cgctgtacgc
     8101 cgcaatatgc atagccgtgg gaaaggtgtc gttgctggat tgtgatttat tgacatcgtc
     8161 attggggtgg agcactttat gcgtatcggt caggctgcct ccggaaagga gatgtgccct
     8221 gttggcaatc acctcgttta cgttcatatt gctttgcgta ccggatccgg tctgccagac
     8281 cacgagggga aactggtcat ccagttttcc ttcgaggatt tcatcgcaga cccgggagat
     8341 cagcatcatc ttttctttgg gcaagacctt gagcttgtgg ttggtgatgg cggccgcttt
     8401 tttcaggatg gcaaaggccc ggatgatttc aatcgggatc cgttgtcccc cgattttaaa
     8461 attctgggtg gaacgctggg tctgggcggc ccaataagca tttgcaggaa cgtctacata
     8521 acccagggaa tctttttctt ttctgaaatc catatcggtt taaaatttca gtaaagataa
     8581 gccggcaaaa tcctcctata aaatcagatt gggaccatgg ccggtgaact gcctggtccg
     8641 gaaatcaacc tttttatttt atttttgttg ctttattaat attcattcac ctataaactt
     8701 aatatcatca tggcatttac gcttccagcc cttccttatt cttttgatgc tcttgaaccc
     8761 catatcgata cccggaccat ggaaatccac cacggcaagc atcaccaggc ttatgtcacc
     8821 aacctcaata ccgccatcga aaacaccgag tgggcggata aatccattga agaaattctg
     8881 gccaacgtat ccaggctaag cccggccgtg agaaacaacg ggggaggcca ttataaccac
     8941 agcctgtttt ggcaaattat gggcccgggt aaaggagggg tgcccaccgg aatcattggc
     9001 gatgccattc aaggcagctt tggttccttc gacgctttta aagaagcact aagcaaagca
     9061 gggatgggcc ggtttggcag cggctgggcc tggctgagca aagatgccca gggtaaactg
     9121 tttatctcct ccactccaaa ccaggacaat ccgctcatgg atcttgcaga acaaaaaggc
     9181 actccgattt taggcgtcga tgtatgggaa catgcttatt accttcatta tcaaaaccgt
     9241 agagccgatt atctaaacgc attctttaat gtaatcaact gggatgctgt tgccgcccgc
     9301 ctctaagaat ttctcagaaa gggagaaagg cgaagggtga aaggcgaagg gcgaaaggcg
     9361 aagggcgaaa ggaatgcaag tacttgctaa ctttccaact ggctaactta ccaacttgct
     9421 aactaattta agctgtaagg gaagggctcg aaccttcacg gagggctttc ccgaagggat
     9481 cactttgtgt cccgaaggga tcactacgtg aagcgcgggt acattgcctg gtggtctccc
     9541 aaggggatca ctttgtgtcc cgaagggatc actatgtgtc ccgtagggat cgctatgcga
     9601 aaaccccagt ctgtccacaa acagggaaag gccttatacc tcatttatag aaaaatctat
     9661 ctggaaaaca ggatcttacg tgatgttcat gcttttcagc caccggtagc catcgatggt
     9721 tttaaaaaaa ttctcccgtc ttagcgcttc tgaattggtt tggaactctt cccaataatg
     9781 caatttccaa ggccggaagg gttttgtata ttttaccctg cccttgttgt gatctgccag
     9841 ccttttttgg atatcgccgc tggatccgta ataatacttt ccgcttaatt cacttcttag
     9901 gatatacgca taatgcatct taagctgtaa gggaagggct cgaaccttca cggagggctt
     9961 agcgcgggta cattgcctgg tggtcaaccc cagtctgtcc acaaacaggg acaggcctta
    10021 tacctggttt atcgccttac tccacccccg agacaagagg gcatgtctgc caatttcatc
    10081 acctcacagt atctcagaat ctcttttgaa tccgatagtg caaatttaaa tgaacggacc
    10141 gaatatttaa agaaaaatgt acgaatatta ttaaatttta tcattcattt aaaaaaataa
    10201 tgattttatt gaataaattt caatttaaca tctctcccgg gcgttccgat tgaaaaaaat
    10261 ttcagctgac cagggactga tcattcattg ggttgaatcg gattataatc tttcaaaatc
    10321 caccgcctca aaggattcga aaagggaggc aggcagggat tcatatacct tgattccggt
    10381 gatctgcctg ttttctcccc ggtccgagga cagaaagata ttgccgtact tcacatatcc
    10441 atcccagggg tttattatgg agggggtgct gtccagtacg ttggggtaat aagcccattg
    10501 cgcgaccagg ctgctgtttt tcccaaccca gacttcatac ttgttttgtg gggtgacccc
    10561 tacgctgtcg aaggtcaggg aaatgacatc cgaggcaatg cccttcacgg tagtatcctc
    10621 ccggacgtac ctcaaatgta caccgggatc tttcatcttg aagggcatgg cgagccaata
    10681 ggaatcgttg atccagatgc gataagcgtt gtcgaggtat tttttcagcg tgtccgggtc
    10741 ctcccattcc cggccgtctt taaagacttt accctgccgg gtattgaggt ccaggatcaa
    10801 tttggtatcg ttgccggtat tttcaatcct gaccttccca tcctttttat cccaaagaag
    10861 ccatctcctg ccgaaaaaat tccatttgat gtaccgggtg tccgcccagg cttccgctcc
    10921 gccggaggcg gcaatcgcgt cttccgctac ccaaatggct tccgggtccg acccttccac
    10981 gttaaatgcc ccgtttatgt tctgcccggt ttggaccggg gtttcatttt tacaagccat
    11041 ccctacggcc aggaaaaggc tgattccaat ataaatgtgg atcctcatat taaaacgatt
    11101 gtcttataaa ggtaatatgc agtcctcatt tacagacatt gggtcgaaag ggggaaaacg
    11161 ggctcggaga tttcgcctat ttttgccgat catattgtaa atccttactg cccttatgtt
    11221 tgaatccatt cacgacaacc tggataatgc ctttaaattc ctgagagggg aaaacaaact
    11281 caccgaactc aatattgccg aggccatcaa ggaaatccgc cgggccctcg tgggagcaga
    11341 cgtcaattat aaaatcgcca aggaattcac ggacaaggtt aaggacaaag ccctgggtac
    11401 ggagaacgtg ctgaaagccg tgaaacccgg tgaattgatg attaaaatcg tatttgacga
    11461 gctgaccgaa ctcatgggag gtcagagcgc cggcatcaat ttaaaagggg aacccggcat
    11521 cgtcctggtt gcaggcctcc agggcagcgg taaaaccacc ttttccgcca aactggccaa
    11581 ttatctcaaa accaaaaaag gtaagaaacc cctgctcgtg gcctgcgacg tttaccgtcc
    11641 ggccgccatt gaccagttga gcaccctcgg ggaacagatc aatgtggctg tattcaagga
    11701 acccgataat aagaatcctc ttgagatagc ccataaagcc ctggcatttg ccagacaaaa
    11761 ccaccatgac gtggtcatcg tggataccgc cggacggacc acgctggatg atgcaatgat
    11821 gaccgaaatc cgtgagatca aagaggcaat ccatcctact gaatccctct tcgtggtgga
    11881 ctccatgatc ggacaggatg ccgtcacgac ggcggccgcc tttcacgagg cgattaatta
    11941 tgacggtgtg gtcctcacca aactcgacgg ggacacccgt gggggggcag ccctgtccat
    12001 aaaatacacg gtgggaaaac ccatcaagtt tatttccatg ggtgaaaaac tggataccct
    12061 ggatgtattt tacccggacc gcatggccca gcgcatcctt ggaatgggtg acatcgtcag
    12121 tcttgtggaa aaagcacagg aacagtttga cgaaaaggaa gctaaagcgc ttgaaaagaa
    12181 aatcaggaaa aataaatttg actttaatga ttttctgtcc cagatcaacc agattaaaaa
    12241 aatgggggac ctgaaaaacc tggtatcgat gatgcccggc atgggaaaag cgctgaaaga
    12301 tgtggacatc aacgacagcg cttttaagaa agtggaatcg atcatccagt ccatgacccc
    12361 aactgagcga gccaatcccg aattgctgaa catgaaccgc aaaaaccgca ttgcccgagg
    12421 ttgtggccaa tcggttcatg aaatcaccgc cttcatcaag cagtttgacc agatgaaaca
    12481 aatgatgcag caactgagca ccggtaaagg catgggcacg atgatgaagc agatgcagaa
    12541 catgagggga ttccagggat aagcgaaata ctttttaaat taataaattc cagactatga
    12601 aaaccatctc aagcctgctt tggtcaggcc tcctcatcct gctcaccgca tgccagccca
    12661 aaaccgcgtc ccgggtggcc accgcacccc cctcccaggt tagccagtcc acgcccactc
    12721 ctgcccagcc ggttacaccc gctttggaag ccccttccga agtcggaatt gatctgggtg
    12781 caaaaattcc actggacccc tctgtccgga ttggggtcct gcccaacggg atgacctatt
    12841 atataaagaa aaacgccaaa cccgaacaaa gggtggaatt gaggcttgcc gtcaaggccg
    12901 gttccatcct ggaggacgaa gatcagttgg gactggccca cttcgtcgaa cacatggcct
    12961 tcaacggcag taaaaatttt aaaaagcagg aactggtgga ttacctggaa tcggtaggca
    13021 cccgcttcgg tcccgacctc aatgcctata ccagctttga tgaaaccgtt tatatgcttc
    13081 aggcccgctc ggatgaagcg gataaacttg aaaaagggct cctggtcatg gaagactggg
    13141 ccagcggggt cgctttcgac ccggtggaaa tcgacaaaga aaggggcgtg gtggtttcgg
    13201 aatggcggac cagcctcagc ggggaacaaa ggatcatgca gaaagtatta ccggtcatcc
    13261 tctaccagtc ccattacgcc aaccgcctgc ctataggcaa acccgaaatt atagaaaagg
    13321 cttcctatga agccgtgaaa cggttttaca aggattggta ccggcccgaa ctcatggccg
    13381 tgatggccat cggcgacctg gatccggata agatggaaac cgaactcagg aaccggtttt
    13441 ctaaaatacc cgcatcaccc gccagtgcgc ggaaaaggga acacatcgaa gtacccatgc
    13501 acccggagac gaaagtgatc gtcgccacgg acaaggaagc agccttcacc caggcccagg
    13561 tctactacaa gcataccaaa aaaacaccgg catcgattca ggattacaga aacggcctcc
    13621 tggccaattt atacaacggt atgctcaatg tgcgtctcga tgaactcgcc cagaaaccca
    13681 atccgccctt caattatgcc ggtgccggat ttggcgattt tctggccaat ctcaatgcct
    13741 attcttcctc ggtgagcaca ccggaaggac aggcgcttac cggccttcag gcggtcatga
    13801 ttgaaaatga aagggctttc aggcatggtt ttacctccac ggaatttgaa aggcaaaagg
    13861 aagacttgat gcgccgtatg gagtccgccg taaaagaaaa agacaaaacc gaatccgggg
    13921 ctattgcttc gacattggtc ggccatttcc tggaa
//
LOCUS       DAOQXT010000114        13851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4899_length_13851_cov_4.275805, whole genome shotgun sequence.
ACCESSION   DAOQXT010000114 DAOQXT010000000
VERSION     DAOQXT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13851
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4899_length_13851_cov_4.275805"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..>1463)
                     /locus_tag="PKM27_16165"
     CDS             complement(84..>1463)
                     /locus_tag="PKM27_16165"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HNR08855.1"
                     /translation="PITVYFLSFGLKSQNLSMLTFILVFLVLAAIIIAIVRYDIHPFL
                     ALFVGAIVYGLLAGMSGKQILDSISDGFGSVVGRIGLVIMLGIIIGTFLEKTGGALVI
                     AQKVLSIIGEKAVMLAMMITGWILSIPVFGDSAFIMMNSINKSLSFKSKAPFAATTIA
                     LTLGISSTHSLVPPTPGPIAAAGILGADLGRVIFWGIIVSSVALIPCYYFVKHYVSRF
                     HLDPKLADEKVENTNAYLPSAAKASLPIIIPLLLIILGSVADYPSHPFGEGRLMAFLK
                     FIGNPIMALLLGAGLSFWLPKKFELRLLSSTGWIGDSLVTAAPIILITGAGGIFGKML
                     QNSGIADLVSSNLSSANLGLLLPFLMSLSLKTAQGSSTVAIVTTASVIAPLLVPLGLD
                     SEFMKVLAVLAIGAGGIAISHANDSFFWCMTQMTGMNIKQGNQTHSLGTLILATGAIA
                     VIYLISWIF"
     assembly_gap    1464..1563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1816..1889
                     /locus_tag="PKM27_16170"
     tRNA            1816..1889
                     /locus_tag="PKM27_16170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1850..1852,aa:Arg,seq:tct)
     gene            2077..2976
                     /locus_tag="PKM27_16175"
     CDS             2077..2976
                     /locus_tag="PKM27_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR08856.1"
                     /translation="MNRYLLILGLAALASSCKNKEPESLQRLHQLEDEYAKSKDPKTL
                     ETLFAEYISFVTGTAGDKNQKIEVLERAEKLSSANNRYFESVTFLNTLIKEDPESPKT
                     GDRLLQLADLMKNVIRNENAANTLYAGFLEKYPSHPKAEVARAVVGDQPTPLRTLIET
                     LAGKMYDDSLHQFNETAATQYVDATEAYVLINPDDADSPELLNKAAETARSLKTYNKA
                     ISLYDWVIEKYPASKVASQSLFLKAFTFDNELHDIENARFYYQEFIKRYPNNDFADDA
                     KVLMENLGKTDQEFLESIQQQNK"
     gene            2980..3378
                     /locus_tag="PKM27_16180"
     CDS             2980..3378
                     /locus_tag="PKM27_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08857.1"
                     /translation="MKSKFFFPALLLALLIVSCSPKTAKPMEAAAAAAPAIPYLGDWT
                     YTVPGTPLGDVTGKMILKSDNGVIKAFLETNGETTELSEFKLEDKNISGIFYYSGTPV
                     RLEGVFESPNSLTGKLTADGAGVFSMKASR"
     gene            complement(3514..4518)
                     /locus_tag="PKM27_16185"
     CDS             complement(3514..4518)
                     /locus_tag="PKM27_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR08858.1"
                     /translation="MIHTDILIIGAGPVGLFTVFEAGLLQMHCHLLDILPQPGGQLSE
                     IYPKKPIYDIPGYPSILASELVDKLMKQIEPFRPGFTLGERAETLFRGDDGKFVITTH
                     KGTRIKAGVVAIAGGLGCFEPRKPPIPGIEKYEDHGVDYFVKNPEVYSGKNIVIAGGG
                     DSALDWAIFLAGKAKTLNLIHRRTEFRGAPDSVAKVMELAKSDQFRLITEAQVSGLAG
                     DNRLEEVIVKHDREGEISIDADYFIPLFGLTPKLGPIANWGLQIEQQAIRVNTLDYST
                     EVPGVYAIGDINTYPGKLKLILCGFHEATLMCQSAFKHLNPDKKLSFKYTTVTGIKGM
                     "
     gene            complement(4931..5551)
                     /locus_tag="PKM27_16190"
     CDS             complement(4931..5551)
                     /locus_tag="PKM27_16190"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR08859.1"
                     /translation="MKGHFPIYIFAEKLDGVNFSTETFWEGKIKEGPNYKFHSKRGYQ
                     YICEATHLNKIPDKKYDFILSCHSLEHSANPLKVVEECKRVLKKGGYFILVLPDKENT
                     FDRKRPFTTMSHLLKDYHCNTGEEDETHFEEVRRLFDFSMVDKSMEMSVWLDQMPNNI
                     NHRLLHHHVFNFELITEIFKFFSFEVIIQKSIKPFHLVTIARCLEK"
     gene            complement(5758..6273)
                     /locus_tag="PKM27_16195"
     CDS             complement(5758..6273)
                     /locus_tag="PKM27_16195"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR08860.1"
                     /translation="MSEQLSLSLWPQTGIFRSLIHFDVVISGKTFEYMEFFWIAMGEM
                     TRVLKRGGLLCIIVPNGFKKHRYPVDYYRFFTDGLVALARYVSLETLHDHTNCAPSIK
                     FKSWYSQKDVDSILIARKNYSGPAKYIDLNSYQCVSPDHEQLKTGLVAYRSIFKRLKS
                     ALKSIFPYRMG"
     gene            complement(6314..7222)
                     /locus_tag="PKM27_16200"
     CDS             complement(6314..7222)
                     /locus_tag="PKM27_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08861.1"
                     /translation="MKFSFLRRRLRTHFKNLSLFYNETNLLAVGSMLCKQQAAINSGS
                     LNDYEFRIFSQWGDDGIIQYLIRNLRIKNETFIEFGVGDYEESNTRFLLMNNNWSGFV
                     MDGSEELVREMTKRNWYWRHDIRARAAFIDRDNINALLAESGFQEVGLLHIDLDGNDY
                     HILEEIDFSRLNPCLLILEYNSVFGAMRPLTVPYDKNFSRGKAHYSHLYYGASLPALQ
                     HLANRKGYALVGGNLAGNNAYFVRRDLLNERVKEVPMEKAYRESRFREARAKDFSLSY
                     LFGPARLELLRGMEVINVVTGGKESL"
     gene            complement(7304..11041)
                     /locus_tag="PKM27_16205"
     CDS             complement(7304..11041)
                     /locus_tag="PKM27_16205"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNR08862.1"
                     /translation="MFQKDQAAYRNQAHARYLKDYTLLKLNPSSFQEIKSRRNSPLSI
                     AIPYQSGQLRMELEPLYLFDASFKLLTDQSGSQGVPYQAGKYFKGRIKDDTTSLVTLS
                     VFEDEVNGIISSARFGNLNLGRIRDQSPEDYIVYNESDAKESVEFTCASLGNAIYRSQ
                     LEQLQSQVDQQLESRADGCITVDFELTREVYSQYQNNINNAANWLTSLFAAVKSLYLN
                     EQVNISIKTIFVWTSEDGYSDDVGTALDQLKARRLNDAAFTANFVHLVRGELCNGGCS
                     LAGIAWLDVLCDNSYRFAVSEPLFSYAVYPSYSWSVNVLTHEQGHNIASPHTHWCGWT
                     GGPIDGCADPDGTCAKGPTPPSGGGTIMSYCHHNVGINFANGFGPQPGTRIRARYNAA
                     NCLSTNCNDGITVPAGPSNCNDGIQNGSETGVDCGGSCAACLEVCANSVNLSQNKTTS
                     QSTNFDGGNSFPASRAVDGSLTNFSHTGQQLQPWWQVDLGNTYTVSSIKITNRVGCAP
                     CSGRLKRFKVFVSNTPPGAYTNSGEVHLYNNSSGLSDGQVYNIANLNASGRYVRIWAD
                     FTGYGNNYLHLAEVQVMGCDGVDPCEENEVPNQPTVSTVEVDFPRNGSFTVGAIASDP
                     DGSIARVEFYNGVTLLGSDNSNPYHWTVSPATASSYSITAKAFDNCGESATSGILTVN
                     TTISCSDGFQNGDEEGVDCGGSSCTDDCPQGCLSPVLLSSGKSTSQSSNFNGSNSYPA
                     SMVVDGSTSSASFNHTAAELQPWWQVDLATVSEVTSIEITHRTGCAPCAGRVKNFKVF
                     VTNSPASSFGSSTGVVYEYNNSTGLGNGQVINISDLAGGGRCGRLWVNNASANYLHLA
                     EVRVMGCATDPCSGNQSPSKPVLSSTSTSYTQNTSFSVNASSSDAGGIAEIRFYVGGV
                     LHSTDNTSPYSMTINPASASSYSITAMAVDDCNAQSVLSDPLVITTTNSCSDGVRNGD
                     ETSIDCGGSNCTACAGCTSPTNVSQGKSASQSTNFNAANSYPASMAVDGSTASSSFNH
                     TGAQTQPWWQVDLGSSHDVTSIQITHRTGCSACAGRIKKFRVFISGSPPSAFTNSGYV
                     YEYNNGAGMANGEVLTIGGDAVTGRYVRIWADYGTSSGYIHFAEVRVFGCASTAPDIL
                     LASTESPVKRFTESRMGLENPVDFEVNIFPNPVRDQLNYNFAIPPQSPVFARIFDLQG
                     RKVMEATLSGSRLDIGHLENGSYIIHIIYKDMRMVKKILKF"
     gene            11410..12531
                     /locus_tag="PKM27_16210"
     CDS             11410..12531
                     /locus_tag="PKM27_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNR08863.1"
                     /translation="MKVLLDYFIFDAAAIGGVYRYFRNLISRSQTSPQLEYQIACLFG
                     EHPEDWKALGLKPKPFHNLSSPILHKASTAFSRGSNILYSLLRMSGSNYDIFHPTYYS
                     LYYQQVVDRIRKPLAVTVYDMIHERYPEYMKKGNTTPQKKKLLCERADVVFAISEHTK
                     KDIVEMLNIPPDKIRVTPLSGGFENPRYNASLAAQLPKKYILYVGGRSGYKNFCLFFR
                     AIAPLLKQDPELTLVCTGGGFNPGERELIKSYALEGKAFAYFVLDQDMHTYYKCAELF
                     VFPSLYEGFGIPLLEAFSSGCPVAASNTSSLPEVGGNAAVYFDPKSESNIASAMQSIL
                     YNPDFKSELIQAGLKRAGQFSWDKTMEITLEGYRSILSR"
     gene            12664..13293
                     /locus_tag="PKM27_16215"
     CDS             12664..13293
                     /locus_tag="PKM27_16215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR08864.1"
                     /translation="MEGKKFLSGTIQNHLVKPITRRVAALRFPSVRFIPLEKTKFENT
                     VPTLSAWKGLEEIIPSILQQFHIPKNHCLEFGVEYGFSTAIFAQLFDEVIGVDTFEGD
                     AHAGYQDTYQKARENLKSYPNVTLVKSSYQEFIHRQDRQYDLCHVDIIHTYKDTFTCG
                     DWAASHCKCVLFHDTVSFPQVRQAVFDIARKHGKIFYNYPFHHGLGIIV"
     gene            complement(13382..>13851)
                     /locus_tag="PKM27_16220"
     CDS             complement(13382..>13851)
                     /locus_tag="PKM27_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08865.1"
                     /translation="KAYLANKSDIDFKKLEGHIDWLNKNKLKLKSKKVGFIEKNYAPK
                     KDDDVQPGERVFYTNSNAKIIDNVRNLIEEIPEPYKTFCLASLIVKASVHNNTSGVFK
                     GFHKKNGIGHFGGQGENALQRIKQEIVLDTPIFSDFECPLPEANTTQLTVQFS"
BASE COUNT         3700 a         3406 c         3091 g         3554 t
ORIGIN      
        1 ggatgaggag gggttcaatc ctcaatcctc aatcctcaat cctcaatcct caatcctcaa
       61 ccctcaaccc tcaaccctca accctaaaag atccaggaaa tcaaataaat caccgcaatg
      121 gcccccgtag ccaggatcag cgtacccagg ctatgagtct ggttgccttg tttgatattc
      181 atgccggtca tttgggtcat gcaccagaaa aaactgtcgt tggcatggga aatcgctatg
      241 ccgccggcgc cgatggccaa taccgcaagc actttcataa attcactgtc caggccaagg
      301 ggtaccaaca gaggggcgat gacggaggcg gtggtgacga tagccaccgt ggaagatccc
      361 tgggcggttt tgagagaaag agacatcaga aagggaagta gaagaccaag gttggcgctg
      421 cttaggttgc tgctcacgag gtccgcgatg cctgaattct gtagcatttt cccgaatatg
      481 cccccggccc ctgtgatcag gatgataggg gcggcggtga cgagggaatc cccgatccag
      541 ccggtggaag acagcagcct caattcaaat tttttgggca gccaaaaaga caggcccgca
      601 cccagcaaaa gcgccatgat cgggtttcct ataaatttca aaaaggccat caatcttcct
      661 tcgccgaagg gatgcgacgg gtaatcggcc acggatccga ggatgatcag gaggagggga
      721 atgatgatcg gtaaggaggc tttcgctgca gaaggcagat aggcatttgt attttcaacc
      781 ttttcgtcgg ccaatttagg atccaggtga aaccgggaga cataatgttt gacaaaataa
      841 taacagggga tcaaagccac tgaacttact ataattcccc agaaaatcac ccggcccagg
      901 tcagcgccca aaatccctgc agctgcaata ggccccgggg ttggcggcac cagggaatga
      961 gtcgaggaaa tgcccagggt aagcgcaatc gtggtggcgg caaagggcgc cttgctttta
     1021 aaagacaggg atttgttgat ggaattcatc atgatgaagg cgctgtcccc gaatactgga
     1081 atggacagga tccaaccggt gatcatcatc gccagcatga ccgctttctc gccaatgatg
     1141 gacagtactt tttgggcgat gaccagggct cctccggtct tttccaggaa ggtcccgata
     1201 atgatcccga gcatgatcac cagcccgatc cggcccacca cacttccaaa accgtcggag
     1261 atggaatcca ggatttgttt cccggacatt ccggcaagaa ggccataaac aatagcaccc
     1321 acaaacaggg ccaggaaggg gtgaatgtca tatcgtacga tggcaatgat gatcgcagcc
     1381 agcaccagga agaccaggat gaaagtaagc atgcttaggt tttgactttt aagcccaaaa
     1441 gataggaaat aaacggtgat gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnagaggcg aactcccaaa ccccagcctg aagactgggt tttgcaccaa tccaagacct
     1621 ctgtattatc ttcatcccct ggcttcagcc aggggcttgg cacctgcagt attaacgggc
     1681 tttagcccca ggctaaccca cccgaccacc gagactcccc ttaccccgtg tgctttcgaa
     1741 atcagtcccg gaccttagtt tggttcaaaa ttcgattatc aaaaaaattt acctttgcgg
     1801 cttaattcta cccctggtcc ggtagctcag ctggatagag caactgcctt ctaagcagta
     1861 ggtcactggt tcgaatccag tccggatcac tttggaaaga ctaggcaggg gttgtcgcag
     1921 gaaactaaga cagggggtga ctttggagac accccctgtc ttagactgcc cgacaccccc
     1981 tacccgcatc ctctcctcat tccttaatta aatcaaaaat aaataaccga tccctgcgtt
     2041 tggtgtattt ttgcgcctca tattttaatt cttggcatga atagatactt actaattctg
     2101 ggtcttgcag ctttggccag ttcctgcaaa aacaaagagc cagaatccct tcagcggttg
     2161 caccagctgg aagacgaata tgcgaaatcg aaagacccca agaccctgga aaccttgttt
     2221 gccgaataca tttccttcgt aacgggcacc gccggggata aaaaccagaa aattgaggtg
     2281 ctggagcggg cggagaaact gagctccgcc aacaaccgat attttgaatc cgtcaccttc
     2341 ctcaatactt tgattaaaga agatccggaa tccccaaaga ccggggaccg cctgctccaa
     2401 ctggcggatc tgatgaaaaa tgtgatccgc aacgaaaacg ccgccaatac cctctatgcg
     2461 ggattcctgg agaaatatcc cagccatccc aaggcggaag tggccagggc tgtagtcggg
     2521 gaccagccca ctccactgcg tacactcata gagaccctgg ccggcaaaat gtatgacgac
     2581 agcctgcacc agttcaacga aaccgccgcc acccagtatg tggacgccac ggaagcctat
     2641 gtgctgatca accccgacga tgccgacagc ccggaactgc tgaataaagc ggctgagaca
     2701 gcccgttccc tgaagaccta caataaagcc atttccctct acgattgggt gatcgaaaaa
     2761 taccctgcca gcaaagtggc ctcccaatcc ttgtttttaa aggcgtttac ctttgacaat
     2821 gagctgcacg atatcgaaaa cgcccggttt tattaccagg aattcatcaa gcggtatccc
     2881 aacaacgatt ttgcggacga cgccaaggtc ttgatggaaa acctgggaaa aacggaccag
     2941 gaattcctgg aaagcattca acagcaaaac aaataaccta tgaaatcaaa atttttcttt
     3001 ccggctctgt tactggccct tctgattgtt tcctgttccc ccaagacggc caagccaatg
     3061 gaagccgctg ccgcggcagc tccggctatc ccatacctcg gcgattggac ctacaccgta
     3121 cccggtactc ccctgggtga tgtcaccggt aaaatgattc tgaaatcgga caacggggtc
     3181 atcaaagcct tccttgaaac caacggagaa accaccgaac tctccgaatt taaactggaa
     3241 gacaaaaata tttcgggcat tttctactat tccggtaccc ccgtccggct ggaaggagtc
     3301 tttgaaagcc ctaacagcct caccggcaaa ctcaccgcag atggcgccgg ggtattttcg
     3361 atgaaagcga gcaggtagga agggagatac cagacgccag acgcgagata cgagaggcca
     3421 gatgccagac gcgagatacc agacgcgagg agcgagaagc gagatgctag caaacttgcc
     3481 aacttgctaa cttgccaact tgccaactaa atcctacatc cccttaatcc ccgtcaccgt
     3541 cgtatactta aaagacagtt ttttatccgg attgaggtgt ttgaaagccg actggcacat
     3601 cagcgtcgct tcgtggaatc cgcagaggat cagttttaat ttaccgggat aggtgttgat
     3661 gtccccgatg gcgtaaaccc ctggcacttc ggtgctgtaa tccagggtat tgacccggat
     3721 cgcctgctgt tcgatctgca ggccccagtt tgcaatgggg cccagcttgg gggtgaggcc
     3781 gaacaaaggg ataaaataat cggcatcgat actgatctcc ccttcccggt catgtttcac
     3841 aatgacttct tcaagcctgt tatccccggc aagcccgctg acctgggcct ccgtgatcag
     3901 cctgaattga tccgatttgg caagctccat tactttagcc acggaatcgg gcgcccccct
     3961 gaactccgtg cgccggtgga tcaggttcag ggtttttgcc ttgccggcaa ggaagatcgc
     4021 ccaatccagg gctgaatccc ctcccccggc aatgacgatg tttttgccgg aatatacctc
     4081 agggttcttg acaaaataat ccaccccgtg gtcctcatat ttttcgatgc ccgggatggg
     4141 gggcttcctg ggctcaaaac aacccaggcc tcctgcaatg gccaccacgc cggctttgat
     4201 ccgggtaccc ttgtgggtgg taatgacgaa tttaccgtcg tccccgcgga acaaggtctc
     4261 cgccctttcc ccaagggtaa atcccggccg aaagggctcg atctgtttca tgagcttgtc
     4321 caccagttca gaggcaagga tggaaggata cccggggata tcgtaaatgg gctttttggg
     4381 atagatttca ctcaactggc cacccggttg tggcaaaatg tccagcaggt ggcaatgcat
     4441 ctggagcagg cccgcctcaa aaaccgtaaa cagccccacc ggcccggccc cgatgatcag
     4501 gatatcggta tggatcataa aagggtgcaa agataggaac ttaccttgtg acaaagtgct
     4561 tcttaaagac acccattgtt ttcgacccac aacctactta ggtcgatgct taaccactca
     4621 tcactcacct ctcaccactc tccccttacc tctcacctct cacctctaac ctttttgacg
     4681 aagccttgct tcggccttat agtcattatt gaaaatatcg atctaacgtt ttccattctg
     4741 cagccactga ttcaagttta acctgactgg acgaagctta gctttgccct ggtagttaca
     4801 ttttaaggaa gtttcgccgt ggtccctcca atatcggcag tacactgtcc tccatccagc
     4861 accatcatcc aatgtaatag atttgacata attggttcct ctcagttttt ttattaataa
     4921 aagtcttttt ctatttttct aaacaccggg caatggtaac gagatgaaaa ggctttatgg
     4981 atttctgaat tatcacttcg aaagaaaaaa atttaaatat ctcagtaatg agttcaaaat
     5041 taaatacatg gtgatgcaac aatctatgat ttatgttatt cggcatttga tctagccaga
     5101 cggacatttc catcgattta tccaccatcg aaaaatcaaa caaccgcctt acttcttcaa
     5161 aatgggtttc atcctcttcc ccggtattac agtgatagtc cttcaacaaa tgggacatcg
     5221 tggtgaacgg tctcttacgg tcaaaagtat tttctttgtc aggcaatacg aggataaaat
     5281 atcctccctt tttaagcacc cgtttgcatt cttcaaccac tttcaatgga ttggcgctgt
     5341 gttcgaggct atgacaggat aaaataaaat cgtatttctt atccggtatt ttatttagat
     5401 gagtcgcttc acagatatat tgatacccgc gcttggaatg aaatttataa tttggtcctt
     5461 ctttaatctt gccttcccaa aaggtttccg tgctaaaatt gacaccatcc agtttttcgg
     5521 caaaaatata aatcgggaaa tgacctttca aattaaaaaa tgcgctgggc cctccgattt
     5581 caagccctga tctaccgtaa aacttcaggg cccaaagaat caattttcgt ttgccaggtc
     5641 ggaaaacaaa ggacacaagg gtgatcatta tatctctcaa actcatttaa cttatgtatg
     5701 atatcctatt ataaaccaaa taattgtggg aagcagacat aaaaatggtt gaatacgtca
     5761 tcccatccgg tatggaaaaa tggattttag ggcagatttc aatcgtttaa agatggatcg
     5821 ataggccact aacccggttt ttaattgttc atgatcagga gatacacact gataggaatt
     5881 taaatcaatg tatttcgctg gtccggaata atttttccgg gcaatcagta tagaatcgac
     5941 atctttttga ctgtaccagg atttaaattt tatacttggt gcacaattgg tatggtcatg
     6001 taaggtttcc aaactcacgt aacgagccaa tgcaactaaa ccatcggtaa aaaatctata
     6061 ataatccact ggatatcgat gttttttaaa accatttgga acaattatac acagcaatcc
     6121 gcctcttttt agaacccttg tcatttcacc cattgcaatc caaaaaaact ccatatattc
     6181 aaaagtcttt cctgaaataa caacatcaaa atgtatcaac gatcgaaaaa ttccggtctg
     6241 cggccacaaa ctcaagctca actgttcgga catcggtcat cacacaccag ccaccatcag
     6301 accctccctg aacctacaaa ctttccttgc cccccgtgac cacattgatc acctccatac
     6361 cccttagcaa ttcaagcctt gcgggtccga acagatagga caatgaaaaa tcttttgccc
     6421 tggcttccct gaacctggat tcgcggtaag ccttttccat agggacctct ttcacccgtt
     6481 cattcaacaa atcccggcgt acaaaatagg cattgttgcc ggccaggttg cctccgacca
     6541 gggcatatcc ctttctgttg gccaggtgct gcagggcagg tagagaagcc ccataataga
     6601 gatggctata atgggctttc ccgcggctaa agtttttatc ataaggtact gtcaggggcc
     6661 gcatggcgcc aaatacactg ttgtattcaa gaatcaggag gcaggggtta aggcgtgaaa
     6721 agtctatttc ttctaaaata tggtaatcat tcccatccag atcgatatgc agcaggccga
     6781 cctcctgaaa tcccgattct gcaagcaagg cattgatatt gtcccggtcg atgaaggcgg
     6841 cccgcgcacg gatatcgtga cgccagtacc agttccgttt ggtcatttcc cggactaatt
     6901 cttccgaacc gtccatgaca aagcccgacc agttgttatt catcaacagg aaacgggtgt
     6961 ttgattcttc gtaatcccct accccgaatt cgatgaaggt ttcattttta atccggaggt
     7021 ttcggatcag gtattgaatg atcccatcgt cgccccactg actgaagatc ctgaactcat
     7081 aatcattcag ggaacccgag tttatggcag cttgttgttt gcacagcatg gaacccaccg
     7141 ctaaaaggtt ggtttcgttg taaaataaac tgaggttttt aaaatgggtt ctcaggcgtc
     7201 ggcgcaggaa tgaaaatttc aaacaaggct tttatattat ttataatagt ttaaccggat
     7261 cgtatttcta ttcaaatatt atgattgtga gaccataaat cctctaaaat tttaggattt
     7321 ttttcaccat gcgcatatcc ttgtaaatga tgtggatgat atagcttccg ttttccaaat
     7381 ggccgatgtc aagccgggat cctgacaagg tggcttccat cacctttctc ccttgaagat
     7441 caaagatcct tgcaaaaacc ggtgactgcg gcggaatggc aaaattataa ttcaactggt
     7501 cacgcaccgg gttcgggaat atatttacct caaaatcaac cgggttttct aagcccatcc
     7561 tggactcagt gaacctttta actggtgact ccgtggaagc gagcaaaatg tccggtgcgg
     7621 tgctggcaca accaaacact ctgacctcgg caaagtggat ataaccggaa gaggttccgt
     7681 aatctgccca tatccggaca tatcttcccg tcacggcatc ccctccgatg gtcagcactt
     7741 ctccattagc cataccagcc ccattgttgt actcatatac atacccgcta ttagtaaatg
     7801 ccgatggagg agatcctgaa ataaagaccc tgaatttttt aatcctgcct gcgcatgctg
     7861 aacaaccggt acgatgagtg atctgaatac tggtgacgtc gtgcgaactt cccagatcca
     7921 cctgccacca gggttgggtt tgtgcaccgg tatgattgaa actcgaactg gccgtggaac
     7981 cgtccaccgc catggaagcg ggataagaat tcgccgcatt gaagttggtg gattgacttg
     8041 ctgatttgcc ctggctgaca ttggtcggac tggtgcatcc agcacaagcc gtacaattcg
     8101 aaccgccaca atctatggaa gtttcgtccc cgttacgaac cccatccgaa caagaattgg
     8161 tagtcgtaat caccagggga tccgacaaaa cggactgcgc gttgcaatca tcaacggcca
     8221 tcgcggtaat tgaataggaa gatgcggagg ctgggttaat ggtcatagaa tagggcgagg
     8281 tattgtctgt ggagtgtaac actccgccga cataaaacct tatttcagcg atcccacctg
     8341 cgtccgacga acttgcattc accgaaaaac tggtattttg agtatagctt gtcgaagtcg
     8401 aactcaaaac cggttttgac gggctttgat tccccgaaca cggatcggta gcacatccca
     8461 ttacccgaac ttcggccaaa tgcaggtaat tggctgacgc gttattgacc cataatcggc
     8521 cacatctccc tccgcccgca aggtcggata tgttaatcac ctggccattc cccagacccg
     8581 tggaattatt gtactcatag accaccccag tggaagaacc aaaacttgat gcgggactgt
     8641 ttgtgacaaa taccttaaaa tttttcaccc ggcctgcaca tggggcgcaa cctgttcggt
     8701 gtgtaatttc aatgctggtt acttcggaaa cggttgccag atccacttgc caccagggtt
     8761 ggagttcagc agccgtatgg ttgaaactag cgcttgaagt cgaaccgtcc acaaccatag
     8821 atgccgggta actattggaa ccattgaaat ttgaagactg actagttgat tttccagaac
     8881 ttaaaaggac cgggctaagg cagccttgag ggcagtcatc ggtacacgat gatcccccgc
     8941 agtccacccc ttcttcatcc ccattctgaa atccatcgct gcaacttatc gtggtattga
     9001 cagtcaggat tcctgacgta gctgattcac cgcaattatc aaatgctttt gcagtaatcg
     9061 aataactgga tgctgttgca ggacttactg tccaatggta tgggttgctg ttgtctgacc
     9121 ctaataaagt gactccatta taaaattcaa cccgggcaat cgaaccgtcg ggatctgaag
     9181 cgatggcacc aacggtgaaa ctgccgttcc gaggaaaatc gacttccacc gtactcaccg
     9241 tgggttggtt aggaacttca ttttcttcgc aaggatccac cccatcgcag cccatgacct
     9301 gcacttccgc gagatggagg taattgtttc catatcctgt aaagtccgcc catatcctaa
     9361 cataacggcc tgatgcattc aggttggcga tgttataaac ctggccgtct gacagaccgg
     9421 aggagttatt gtatagatgt acctctccgg agtttgtgta agccccaggt ggggtgtttg
     9481 agacaaatac cttaaatcgt ttcagccgtc ctgaacatgg agcacatcct acccgattgg
     9541 taatttttat actcgacacg gtataggtat ttccaaggtc aacctgccac cagggttgta
     9601 gttgttgtcc ggtgtggctg aaattggtca atgaaccatc aacagcccgg gaggcaggaa
     9661 aactgttccc accatcaaaa ttggtcgatt gactggtagt cttattttga ctaagattta
     9721 cagaattagc acacacctca aggcaagcgg cacaacttcc accacaatcg acacccgttt
     9781 cgctcccgtt ctgaatcccg tcattgcaat tggagggccc ggcaggaacg gtgatgccat
     9841 cgttgcaatt ggttgatagg cagttggctg cattatacct ggcccgaatg cgggtacccg
     9901 gttgtggccc gaaaccgttg gcaaaattaa tcccgacgtt atgatggcaa taggacatga
     9961 tggttcctcc ccccgagggt ggggtcggcc ctttggcaca ggtcccgtca ggatcggcgc
    10021 aaccatcgat aggtcctccg gtccatccgc accaatgggt atggggagaa gcgatattat
    10081 gcccctgctc atgagtgagt acattgacgg accaggagta ggagggataa accgcgtaac
    10141 tgaacaaagg ctcgctcacc gcaaagcggt aactgttgtc acataaaaca tccagccagg
    10201 cgattccggc caggctgcag cccccgttgc aaagttcacc cctgaccagg tggacaaaat
    10261 tggccgtaaa ggcggcatcg ttcaatcttc gtgctttcag ttgatccagg gcagtcccca
    10321 catcgtcgct gtatccgtcc tccgaggtcc agacaaaaat ggttttgatg ctgatgttga
    10381 cctgctcgtt cagatagagg gatttgaccg cggcaaaaag ggaggtcagc cagttggccg
    10441 cattattgat attgttttga tattgggaat agacttcgcg ggtcagctcg aaatccaccg
    10501 tgatgcaacc gtccgccctg ctttccaatt gctggtccac ctggctctgt aattgctcca
    10561 gttgcgaccg gtaaatggca ttaccgaggc tcgcgcaagt aaactccacg gactccttag
    10621 catcggattc attgtatacg atataatcct ccggagactg gtcccggatc ctccccaggt
    10681 tgagattccc aaaacgggcg ctggagatga ttccgtttac ttcatcttcg aatacggaca
    10741 gggtcaccag ggaagtcgta tcgtctttta tccggccttt gaaatacttg cctgcctggt
    10801 aagggacccc ttgactcccc gactggtcag tgagtagttt aaacgaagcg tcgaataagt
    10861 acaggggctc cagttccatt cttaactggc ccgattgata gggaatagct atggacaagg
    10921 ggctgttccg ccggcttttg atctcttgga aactggaagg attgagctta agcagggtat
    10981 agtctttgag atacctggca tgggcctgat tccggtaggc agcctgatcc ttttgaaaca
    11041 aaacaatggt tgaatcctgc gacagtctca atttacccag aaatggaaca ccgtaaattg
    11101 gagataaagc aatatttaat accgaaagga gagcaaacaa gggcacgaaa aacatatctt
    11161 ccgcttttaa tacaatataa aagattataa cttaatttta acaatcttta ttgtacttta
    11221 tttaacaaaa cattaataaa atcaaaacgc cttgtgtaaa gggaaagaag aagtcaaaat
    11281 tacgaaaatt tatccttttt atgcttgccc tgcggttgat ttgttggcac catcccaatt
    11341 tcctttgtgg tgccctaacg gttggttaaa ccgacaacta ttatatttgc ccggactgag
    11401 gacaagtttg tgaaggtact cctggactat tttattttcg atgctgcggc tatcggcggg
    11461 gtttaccgtt attttcgtaa cctgatcagc cggtcgcaaa cctcgccgca actggaatac
    11521 caaatcgctt gcctttttgg cgaacacccg gaggattgga aagccctcgg tttaaaaccc
    11581 aaaccctttc acaacctttc cagtcctatc ctgcacaagg catccacggc cttttcaaga
    11641 ggctccaaca tcctctattc cctgctccgg atgtccggat caaattacga cattttccat
    11701 cccacttatt attcattgta ttaccaacag gtggtcgacc ggatccgtaa accgcttgcc
    11761 gtcacagtgt acgacatgat ccatgagcgg tatcccgagt atatgaaaaa aggaaatacc
    11821 actccacaga aaaagaaatt gttgtgcgag cgggcggatg tcgtgtttgc gatttccgaa
    11881 cataccaaaa aagacattgt ggaaatgttg aatatccctc cggacaagat aagagtcacc
    11941 cccctttcag gaggctttga aaaccccagg tacaatgctt ccctagcggc tcaacttcca
    12001 aagaaataca tcctttatgt tggcgggagg tcaggatata aaaatttctg cttgtttttc
    12061 cgggccattg cccccttgct taaacaagac cctgaattga ccttggtttg taccggcggg
    12121 ggattcaacc ctggagaacg ggaattgatc aaaagttatg cattggaagg caaggcgttc
    12181 gcatattttg tccttgatca ggacatgcat acttattata aatgcgctga gctgtttgtt
    12241 ttcccatcgc tttatgaagg atttggaatc cctttgcttg aggccttttc cagcggctgt
    12301 ccagtggcag caagcaatac cagctctctc cccgaagtgg gtgggaatgc ggccgtttat
    12361 tttgatccaa aaagcgagag caacattgcc tcagcgatgc aaagcatctt gtataaccca
    12421 gattttaagt cagaactgat ccaggcaggc ttaaaaaggg cgggtcaatt ttcttgggat
    12481 aagactatgg aaatcacttt ggaaggatac cgttcaatcc tttcgaggtg aatcgggaac
    12541 cggccgttca ggaaaaagac tttcttgggc ttccgtaaat atattgaaat caaccatcat
    12601 ttattgattt gtaaatcagg catttaaagt atatatttga cctttataac catttagcca
    12661 ctcatggaag gcaagaaatt tttatctggg accattcaaa atcatttggt caagcctatt
    12721 actaggaggg ttgcagcgct gcggtttcct tccgttcgat ttattccact cgaaaaaaca
    12781 aaatttgaaa acaccgtgcc caccctttca gcctggaaag gcctggaaga aatcattcct
    12841 tccatccttc aacaattcca tattccaaag aaccactgtc tggaatttgg tgtggaatac
    12901 ggcttttcca ccgcaatttt tgcccaactc tttgacgaag taattggggt agataccttt
    12961 gagggtgacg cacatgcagg ctatcaggac acttaccaaa aagccaggga aaatctcaag
    13021 tcttatccga atgtcacgtt agtcaaaagt tcataccagg aatttattca tcggcaggac
    13081 aggcaatatg atttatgcca tgtagacatc attcacacct acaaggatac ttttacttgt
    13141 ggagattggg cagccagtca ttgcaaatgt gtattatttc atgacaccgt cagttttcca
    13201 caggtccgtc aggccgtatt tgatatagcc aggaagcacg gcaagatatt ttataattac
    13261 ccatttcatc atggacttgg aataattgtt tagttcagga tttgaccccg ttccctaagt
    13321 cttggatcct ggctcaaatc caagaagtaa tgaatgaagc acagggtcaa taaaggaatg
    13381 cctatgaaaa ttggactgtt agttgtgttg tattcgcctc aggaagggga cattcaaaat
    13441 ctgaaaaaat tggtgtgtct aaaacaatct cctgtttaat tctttgaagt gcattttctc
    13501 cttgtcctcc aaaatgtccg attccatttt ttttgtgaaa tcccttaaat actccagatg
    13561 tattgttgtg tactgaagcc ttaacaatga gagaggcgag acaaaaagtt ttgtaaggtt
    13621 ctggtatttc ttcaattaaa tttcgaacat tatcaataat ttttgcattt gagtttgtat
    13681 aaaaaactcg ttcgcctggt tgaacatcat catctttctt gggtgcataa tttttttcta
    13741 taaatccaac ttttttggat tttagtttca atttattttt gttcaaccag tcaatatgtc
    13801 cttcaagttt tttgaaatct atatctgatt tgttggctag atatgctttg t
//
LOCUS       DAOQXT010000115        13755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4940_length_13755_cov_6.868248, whole genome shotgun sequence.
ACCESSION   DAOQXT010000115 DAOQXT010000000
VERSION     DAOQXT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13755
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4940_length_13755_cov_6.868248"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1195)
                     /locus_tag="PKM27_16225"
     CDS             complement(260..1195)
                     /locus_tag="PKM27_16225"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNR08866.1"
                     /translation="MKTVSKWVGIILALVLVVGAIAAFYLKSKFENLSKRTLEPKVTA
                     VPILSDSASLERGKVLAVACRNCHGNDYAGLDFFNDPSIGYMSSPNLTGAKGSATENY
                     GDLDWTRTLRHGVNPSGKPLMVMPSEVIGQLSDQDLGSLIGYMKTVPEIEKPLGATQF
                     TFMAKILAGAGMFGNLYPCDIIKHDEVQSIPHVEISNSPEYGAYFVRYEGCKSCHGAT
                     LNGGVSPDPISPPGLNITPAGNIGKWTLDQFRETVRSGKTPEGKILDPKFMPWPGIGA
                     HNDIELEGLFNYLKSQPALANSPELDKKLKDMAKK"
     gene            complement(1318..1698)
                     /locus_tag="PKM27_16230"
     CDS             complement(1318..1698)
                     /locus_tag="PKM27_16230"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR08867.1"
                     /translation="MKKIILFAFIFLNGLHWGAPGNSLLAQDVHMTASNVYKLVADSL
                     GMRIFETVWTPGAKAAFHTHPDYAAYVVEGGTLDIIEADGKKTTVELVPGMALIAGTE
                     SHSAINTGKTTIKVVVVETTRPRK"
     gene            complement(1731..2180)
                     /locus_tag="PKM27_16235"
     CDS             complement(1731..2180)
                     /locus_tag="PKM27_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08868.1"
                     /translation="MKIKKGVRFILALSGWLFAWPGSAQTVSMLSSRSMESDILTPAS
                     PAPAAIEKTNFVVRDIFRLQYGHYRDAIALIDEAIKSNMFPPNGRILTDFTGEAYRLI
                     LEAGYHTLADFEKAINTELGQPGWRDWYEKFKVHVESSSREILRWVR"
     gene            complement(2207..2503)
                     /locus_tag="PKM27_16240"
     CDS             complement(2207..2503)
                     /locus_tag="PKM27_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08869.1"
                     /translation="MYIVRDIFNLRYGMFKEAMTSIRKAVQVGLLPVKDIRILSDFTG
                     DAYRLIMESEAESLAAYEKEMNEGMKDPRFQEFYSEFKTYVSSGHREILRRHQL"
     gene            complement(2605..3231)
                     /locus_tag="PKM27_16245"
     CDS             complement(2605..3231)
                     /locus_tag="PKM27_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR08870.1"
                     /translation="MKTEIVIYEDNAFLRQSIAELIQEDGRFRVTGDFDNCNQVISQL
                     QKLHPRVVLLDIGLPGLSGLEALKGIKAHFPEIDVIMLTVFEDNENIFEAICAGASGY
                     LLKKTPPQKIPEAIEEVLHGGAPMTSSIARKVLHLFQNNLRPSKELDRLGPREQEVLQ
                     LLTSGHSYKMIAQKCQITVETVRTHIKRIYEKLQVHSATEAAAKAFRK"
     gene            complement(3292..6306)
                     /locus_tag="PKM27_16250"
     CDS             complement(3292..6306)
                     /locus_tag="PKM27_16250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNR08871.1"
                     /translation="MFTVLELSGQPADLHFKHYAYPEGLTAPVRKIQQDLTGFIWIGT
                     EDGLNRFDGKSFTVYRNSPADTNSLTNNIVNDLNTDSQGRVWVANNGGLCYFSYRDHR
                     FHRITLPDNLEPDDRYRIHGVASNAEGEIWFATKNKIHLLDSSLQIHSSHQLPGDKNV
                     FIRDLFTDRKSQVWVGANYNGIFLFDSESGTFKKGSPHSTYAGIQNLTITTNPMTRLA
                     GDTLLAGTWYGGLQLLFPWQDHIMSREIPNPLEADHKKNIITSVCRWKDDLWWIGSHG
                     CGLFLFDAKRKIFVRQYRHQAGEARSLGNDFVGDVFVDRQGIVWIGTEGGLDQYDEKS
                     EQFSLVAIRTKPGETSVARLPRAIVEDPSLPDSYWVAVPGIGLLRFKHEGRLVEQPLP
                     SWAARLPDKNVYCIFYENPASLLIGTLTGVFRVYPGLDQIKAVSFPGNQVPVGVNHIL
                     RDKKQRLWFSTFSNGVFCFDSLSGQLKHFSQNPDQGPALPDNKIFCLLEDHAGNIWAG
                     TQNRGLCRIRADLADILTLEYRQYKNHGLPDNNIYDLYEEGEKRLWVATENGLACIRP
                     GGQPIQSFDMRDGLSNHVIYSITPGKAGHLWLATNNGLCDFDTRSYKSKNHFIRMGLP
                     NNRINGASLAARNGYLFFTTGSHILVCDPGKLEFSGSLPPTVVTGIRVFENSYPFIQN
                     TTGIGPIRLTYRQNNIGFEFTTLNFTNPESNQFAYKMEGLTEEWVNCGSRQMIGFTNL
                     SGGHYLFQARAIHADGKWTESLPVLIYIAPPFWLRAWFIGLVLTVLGCAAYGIMRFRI
                     NQLIRLQKIRLNIARDLHDDIGSTLSSIHMVSTMSEEKQKGRPEANAFATISKASQQA
                     MELMNEIVWSINPKNDTLDMMLIHMRQFTSEILEPLNIHCHFIQPEACNSLVIPLEKR
                     KDFYLIFKEAINNIAKYSGATKVTVELACHLKSLSLTITDNGRGFDPLGKHKGNGLKN
                     MEARARMIGGQLEIDTGPGKGTRTQLAFPLVP"
     gene            complement(6483..7082)
                     /locus_tag="PKM27_16255"
     CDS             complement(6483..7082)
                     /locus_tag="PKM27_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNR08872.1"
                     /translation="MKFSNLILILALTGPVTVLHSQKMKNIFNGKNLDGWKVPENNTW
                     YTASKGILNIQSSPDRKGSILWTSKDYRNFIMQADFKMGAGTVDSGIFLRGENDQIQI
                     GISGSLKRDMTGSPYIPVLRYPVEAKGVKEILDEKGWNTMKVKVVDKTYTVWLNGREV
                     MTYTSEKIPESGPVGLQLHPGNDMQIQYRKIRLGEISGQ"
     gene            complement(7128..8330)
                     /locus_tag="PKM27_16260"
     CDS             complement(7128..8330)
                     /locus_tag="PKM27_16260"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNR08873.1"
                     /translation="MCKFHHPGTGLLFLFSTVWIVSMDRLPAQADFRPGYLVYNSGDT
                     LRAWINVEKTHVCEFKLGREKKQLGPTEITGYGFENDRVFSSTVLSGRFLEVIVDGFL
                     SLYKSDNVYYIKKSMDTLIRLEEKQGTESIHWKGILRLVLKDCIDRPEDIATMRFSES
                     DLLKKVIAYNECKQENYIEYKNSLPSLRIRPGLGFGTGYSWFELRNGETVTVIPTKFR
                     SLDPIPVLLLNFYSPKVQNRISIQTEIQWKRLLTSSEIEFSQPPNKILNRETMINLSF
                     LSLPVYVKYTLPMDKKTMAFYFGFLYDILLHQKTKVLTAEYLDQTVNHTELIDPYDFT
                     LPQFGFSTGLSYGLLLGKISLSTHLKYNFQGLTADSLLRKDGKFAYHARRSSLWNRMS
                     LVLAIQMR"
     gene            8521..11919
                     /locus_tag="PKM27_16265"
     CDS             8521..11919
                     /locus_tag="PKM27_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR08874.1"
                     /translation="MKIRFTLLAFPAFIFLMLAACRKGQAPAAVDLLTITQDSASARK
                     MAEEIKSRSALKLAPGLQIQVWATDSLAPDPVAMDIDAQGNIYLTRTNRQKNSEFDIR
                     GYPHWMTASIALQSVEDRRKFLHETFAPEKSEENKWLTDLNQDSIHDWRDLAVEKEEV
                     WKLEDRDQDGLAEVSTRVINDFNDEVSDILGGILVREKDMFLAIGPDLWHMEDTNGDG
                     VMDKKTSISHGYSVHIGFSGHNMSGVIEGPDGKIYWNIGDIGASITDVQGNKYHHPNE
                     GMILRSNPDGSNFEVYASGLRNTHEFVFDEYGNLISSDNDGDHRGERERLVHIVEGSD
                     AGWRSNWQYGKYTDPKNNGYKVWMDERLSVPRWEGQAAYIIPPIQNFHNGPTGMVYNP
                     GTALGSEWLKKFFLVEFVGNPASSHIWAFSLKPKGASFDLDTDLDMVSGFLPTGIRFG
                     VDGALYAADWVNGWNTKNYGRVWKIDVAEDKNDLKVQRAETRKLIGLKYGTLDEARLE
                     TLLKYPDMRVRLKAQFELARRDKKGYPQFKNAIAQKESQLARVHGIWGIGQLLDKNKG
                     YAQDLIALLTDPDEEIIAQALKVLGDRRIESAGETMIPFLKHANPRIRFFAAQSLGRI
                     KHQLAQDALIELLRTNADEDVYIRHASVLALTRLGNASRLASLASDASRAVRIGGVLA
                     LRRLKSPEIRVYLKDQDEYVATEAARGINDDESIPAALPDLAEMVNQTAFKSEPLLRR
                     AINACLRVGEDRHLDMLIAFAQRSDISNVLKAEAMSTIGTWANPSVLDRVDGRYRGEI
                     KREETVVRQKIGPKLKDFLSSSDPDILIATSKMIAELQLAEHASDLESIFVKNDSADV
                     RVAALNALEQIKATTLAQLIPKGMKDPSPKVRAAALGMLDKIELSGAELGRTVNEFLS
                     KGSPREQQRLMGVLGKLPITKTESILSGLIEDLKAKKLPAALGLELKEAIEQSGSAVL
                     MDKLKAVRSGNDPMGEYTLALEGGNPFAGYRYFNESTTGQCIRCHTVNGAGSNVGPDL
                     SKIGSTLTREQILKSLVDPSARLAPGYGVVVLTLKDGSEVYGKLDQENEKELVIITSD
                     AEPLRIATERIRERKNIPSSMPVMTDKMTMREMRDVVEFLATRK"
     gene            complement(12051..13484)
                     /locus_tag="PKM27_16270"
     CDS             complement(12051..13484)
                     /locus_tag="PKM27_16270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR08875.1"
                     /translation="MLLIQLTHCTFCLGTQTMLSDFSWSLKAGQQWLVAGPSGSGKST
                     FFKALHGEFRLTGGRFLFKGAEDMEAFQKLKQATGFVFFQDKAPDPNQFYYQQRYNSQ
                     DADDTLTVRDYLFDGKPMEDREQEGSLHMFHVEDQLDKELIKLSNGENRKVRIIKALL
                     KNPEILVLDSPYTGLDKASRLELNDLIDRLAGAGIHVLLTAPHGQVPVSMTHVLQLEQ
                     SKITYAGTRQSFNRPGNPAQKTKIQIPFRPPENNDFDIAVRLNRVTIRYEETLLLDRI
                     NLTIRRGEKWLLTGPNGAGKSLIASLIYADHPQSYLNDIILFDHARGRGESIWDIKDR
                     IGFLSPEFHFYLDQEQTARQVAAGGLRDNPYAPKTLTPDTESLIKDLFRYYQMEDFID
                     APMYALSTGLQRLVLFIRVIVKNPAFLLLDEPFQHFDLGMIEKSKDLLDAFCEDRTLL
                     FISHEDAEVPGIVRKGKKMELSRGTAK"
BASE COUNT         3344 a         3407 c         3580 g         3424 t
ORIGIN      
        1 cgaagggtta ggtcatgctt tatattttct tggtggtctt cgtgtcttcg tgggcagcaa
       61 ctttgggtcg cggtgggaat aagccgctat gcggctttcc cgctaatatt tcccacgacg
      121 acacagcgaa gcgaaggcgc aaaggggaag gtgatccctt acattttctt ggtggtcttc
      181 gcttcgctgc gtctttgtgg gcgccaactt agtgtcaccg tgggaatgag ccgctacgcg
      241 gcgatcccgc taaatgcaat cactttttag ccatgtcttt cagcttcttg tccaattcag
      301 gtgaattggc cagggcgggc tgggatttga ggtaattgaa taacccttcc agttcgatgt
      361 cgttgtgcgc cccgatgccc ggccagggca taaacttggg atcaaggatt ttgccttccg
      421 gtgtttttcc ggaccgtacg gtttctctaa actggtccag ggtccatttg ccaatattgc
      481 ctgccggagt gatgttcaag ccgggaggag agatcggatc gggggagact cctccgttta
      541 gggtggcgcc gtgacaggat ttacagcctt catatcgtac gaagtaagcg ccatattccg
      601 ggctgttgga gatttcaaca tgaggaatgg attgaacttc atcatgtttg atgatatcgc
      661 aggggtagag attgccaaac atgccggcgc cggccaggat tttagccata aaggtaaatt
      721 gggtcgcgcc cagtggtttc tcaatttcgg gtaccgtttt catatatcca atgagactgc
      781 ccaaatcctg gtcgctcaat tgaccgatca cttcggaggg catgaccatc aagggtttcc
      841 ctgacgggtt gacgccatgc cgcagcgtcc gggtccagtc caggtcaccg taattttcgg
      901 tggcggatcc ttttgcaccg gtaaggttag ggctggacat atacccaatg gacggatcgt
      961 taaaaaaatc caagccagca taatcgtttc catgacaatt ccggcaggct accgccagca
     1021 cttttcctct ttcgagggag gcactgtcgg acaggatggg taccgctgtg actttgggct
     1081 caagagttcg cttggataaa ttttcaaatt tggatttcag atagaaagcg gcgatggctc
     1141 cgactaccag gacgagggca agaatgattc ccacccattt tgatacggtt ttcatattta
     1201 aggttttgtt gctacgaata tagcaacaac caaccgtttt ttaacacttc ctacctggcc
     1261 agatttgccc caagcattcg gtggcaattg gaaattcagg atttttcttg aggaaggcta
     1321 cttccggggc cgggtggttt ccaccactac caccttgatc gtagtcttgc cggtattgat
     1381 cgccgagtgc gattccgtgc ctgcaatcaa agccatcccc ggcaccagtt caacagtggt
     1441 ttttttccca tccgcttcga taatgtccag ggttcctcct tccactacat aagcggcata
     1501 atccggatgg gtatgaaagg cggctttggc accgggcgtc caaacggttt caaatatccg
     1561 cattcccagg ctgtccgcca cgagtttgta aacattcgag gccgtcatat gtacatcctg
     1621 ggccaggagc gaattgcccg gagcccccca atgcaggccg ttgaggaaaa tgaaagcgaa
     1681 taaaatgatt tttttcatat ttgggttttt aaggattatg acctggaaga ctacctcacc
     1741 cacctcagga tttcccggga tgaggattcg acatgcactt tgaatttttc ataccagtcc
     1801 ctccatcccg gctggcccaa ttcggtatta atcgcttttt caaaatcggc cagcgtatga
     1861 tatcccgctt caaggatcag ccggtaagcc tccccggtga aatcggtcag gatgcggcca
     1921 ttcggcggga acatgttgga ttttatggct tcatcgatca aggcgatggc atcgcggtaa
     1981 tggccatatt gcaatctaaa aatgtccctc accacaaagt ttgttttttc aatggcggcc
     2041 ggggcggggg aagcaggagt taaaatatca gattccatac tcctggacga aagcatgctt
     2101 accgtctggg cacttcccgg ccaggcaaag agccacccgg ataaagcaag aataaaccgg
     2161 actccttttt taattttcat gatttttggg gtttatggct taaagtttag agctggtgcc
     2221 tccgcaggat ttcccggtga ccggaagaca cataggtttt gaattccgaa taaaattcct
     2281 ggaaccgggg gtctttcatc ccttcattca tttccttctc ataggcggcc agggattcgg
     2341 cttcggattc catgatcaag cgataggcat cgccggtgaa atcactgagg atacggatat
     2401 cctttaccgg gagcaggccc acctggactg ccttccggat cgaggtcatc gcctctttaa
     2461 acattccata cctcagatta aaaatatcgc gtacgatata catggaaaag gttttttggg
     2521 gtttgccttt caggattaat gaacaggacg aaaatgagaa atccccccgg gccggtcaat
     2581 cccataagta tgggacaagc cggtttattt tctgaaggcc ttggctgccg cctcggtggc
     2641 tgaatgcacc tggagctttt cgtaaatcct cttaatatgg gttcgcacag tctctacggt
     2701 aatctggcat ttctgggcga tcattttata actatgacct gaggtgagca gctgcagcac
     2761 ttcttgttcc cgcggaccca agcggtcgag ctctttggag ggccttaaat tgttttggaa
     2821 cagatgcaat actttacggg caatggagga agtcataggc gctccgccat gcagcacttc
     2881 ttcaatggct tccgggattt tctgcggcgg tgtttttttc aacaggtatc ctgaagcccc
     2941 ggcacagatg gcttcaaaaa tattttcatt gtcttcaaaa acggtgagca taatgacgtc
     3001 gatttccgga aaatgggctt tgatcccttt taaagcttcc aggccgctga ggccgggcag
     3061 gccaatgtcc agcaagacca ccctgggatg cagtttctgc aactggctga tcacctggtt
     3121 gcaattgtca aaatcacccg tcacccggaa cctgccgtcc tcctggatca gttctgctat
     3181 actctgtctg agaaaagcat tatcctcata gattacaatt tctgttttca cgggcgggtg
     3241 caagttaact cataaaggta ttttgatcct ggatgcctgt caatcccacc attatgggac
     3301 aagcgggaag gccagttgag tccgggttcc ttttccaggc ccggtgtcga tctccagttg
     3361 tcccccgatc atccgtgcgc gggcttccat gtttttcaag ccgtttcctt tgtgttttcc
     3421 caggggatca aatccacggc cattgtcggt aatggttaaa ctcagggatt tcagatggca
     3481 ggccaattca acagtgactt ttgtggcgcc ggagtatttg gcaatattgt tgatggcctc
     3541 tttaaagatc aaataaaaat cttttctttt ttccaggggg atgaccaaag agttgcaggc
     3601 ttccggttga ataaaatggc agtggatgtt caggggttcc aggatttcag aggtgaactg
     3661 acgcatgtgg atgagcatca tgtccagggt atcgttttta ggattgatgg accagacaat
     3721 ttcattcatg agctccatgg cctgctgtga ggctttcgaa atggtggcaa aggcattggc
     3781 ttctggccgg cccttttgtt tttcctccga catggtgctc accatgtgta tgctgctcag
     3841 ggtggacccg atgtcgtcat gcaggtcgcg ggctatgttg agcctgatct tttgcaaacg
     3901 gatcagctgg ttgatgcgaa atcgcatgat cccataggcc gcgcagccca ggacggtcag
     3961 caccaggccg ataaaccagg cccggagcca gaacgggggc gcgatgtaaa tgaggacggg
     4021 aagggattcg gtccactttc catccgcgtg aatggccctg gcctggaaca ggtaatgtcc
     4081 tcccgaaaga ttggtaaaac cgatcatttg ccttgaaccg cagttgaccc attcttcggt
     4141 cagcccttcc attttatagg caaattgatt ggattccggg ttggtgaaat taagggtggt
     4201 gaattcaaag ccgatgttgt tttgccggta cgtcagcctt atcggtccaa tgcccgtggt
     4261 attctgaatg aaaggataag aattttcgaa tacccggatg ccggtcacta ccgttggggg
     4321 aagggatccg ctgaattcaa gtttcccggg atcacagacc aggatatgac tgcccgtagt
     4381 aaaaaacaaa tagccattcc gggcagccag ggacgccccg ttgatccggt tattgggcaa
     4441 gcccatgcgg ataaaatgat ttttggattt atacgaacgg gtgtcaaaat cgcacaggcc
     4501 gttattggtt gcaagccaga gatgtcccgc tttgcccggg gtgatggaat aaatgacatg
     4561 attgctgagg ccgtcccgca tgtcgaagga ttgaatgggc tgtccaccgg gccggatgca
     4621 ggccaggccg ttttccgtgg ccacccaaag acgtttttcc ccttcttcgt agagatcgta
     4681 gatattgtta tcggggaggc catgattttt atattgccgg tattccaggg taaggatgtc
     4741 cgcgaggtcc gcacggatcc tgcaaagccc ccggttttgg gtgccggccc agatgttccc
     4801 ggcatggtct tcgaggaggc aaaagatttt attgtcgggc agggcagggc cctggtccgg
     4861 attctgtgaa aaatgtttta attgcccgga aagggaatca aaacagaaaa cgccattgga
     4921 aaaggtggaa aaccacagcc tctgtttttt atccctgaga atgtggttga cgcccaccgg
     4981 cacctggtta ccggggaagg ataccgcttt gatttgatcc aaaccgggat agacccggaa
     5041 tacaccggtt agggtaccga tcaaaaggga agccgggttt tcataaaaga tgcaataaac
     5101 gtttttgtcg gggaggcgcg cggcccagga aggcaagggc tgttctacca gtctgccctc
     5161 atgtttgaac cggagcaatc caatacccgg aacagcaacc caatacgaat ccggaagcga
     5221 tgggtcttcc acaatggccc tcgggagacg ggctacggaa gtttctccgg gtttggtacg
     5281 gatggcgacc aggctgaatt gttccgattt ctcatcgtac tggtccagcc cgccttccgt
     5341 tccaatccac acgataccct gccggtcgac aaaaacgtcg cctacgaaat catttcccag
     5401 gctgcgggct tccccggcct gatgacggta ttgccggacg aatatttttc gcttcgcatc
     5461 aaaaagaaaa aggccgcagc cgtggctgcc aatccaccag agatcgtcct tccagcggca
     5521 aaccgaggtg atgatgtttt ttttgtggtc cgcttccaat gggttcggga tttccctgct
     5581 cataatgtga tcctgccagg gaaacaaaag ctgcaatccc ccgtaccagg ttccggcaag
     5641 gagagtatcc cctgcaaggc gggtcatcgg gttggtcgtg atggtcaggt tttgaattcc
     5701 ggcataggtt gaatgcgggc ttcccttctt aaaagtgccg gactcgctgt caaataaaaa
     5761 gatcccgtta taattggcgc ccacccatac ttgtgacttc cggtcggtga acagatcgcg
     5821 aataaataca ttcttatcgc ccggtagctg atggctggaa tgaatctgaa gagaggaatc
     5881 caacaggtga attttatttt tggtggcaaa ccagatttcc ccttctgcgt tggaagccac
     5941 tccatgaatc cggtaacggt catccggttc caggttgtcc ggcagggtga tccggtgaaa
     6001 gcgatggtcc cggtaggaaa aatagcatag ccctccattg ttggctaccc agacccgacc
     6061 ctggctatcg gtattcagat cgttgacaat attattagtc agggagttcg tatccgccgg
     6121 agaattccgg taaacggtaa aactttttcc atcaaaccgg ttaagaccgt cctccgtccc
     6181 gatccagata aagccagtca ggtcctgttg gattttacgt accggcgcgg taagaccttc
     6241 cggataagca taatgtttga aatggaggtc agccggctgc ccgctaagct ccaagacagt
     6301 gaacactgaa aaaatataac cgaccgagta ttttaatttc cagttcaaag ccgatgaacc
     6361 attttcaatt aaagggaatg aaaataaatc ttcccgccgt tatcccccct gacccctgac
     6421 ccctgacccc tgacccctga cccctgacca ctgactactg actactgact actgaccact
     6481 gactactgac cactaatttc tcccaacctt atcttcctgt actgaatttg catgtcgttg
     6541 cccggatgca attgtagccc gaccgggccg gactcgggta ttttctccga agtataggtc
     6601 atcacctccc ggccgttgag ccataccgta tacgtcttgt ccaccacttt tactttcatg
     6661 gtattccagc ctttttcatc gaggatctct ttcactcctt tcgcttccac cggataacgc
     6721 agtacgggga tatacggtga gcccgtcata tcccgtttga gggaaccgct gatcccgatc
     6781 tggatctggt cattctcccc gcggagaaag atgccggaat ccaccgtccc tgcgcccatt
     6841 ttaaaatcgg cctgcataat aaaatttcga taatccttgg aggtccagag gatcgaacct
     6901 ttgcggtccg ggctgctttg aatgttcaga atgcccttgg aagcggtgta ccaggtattg
     6961 ttttctggga ctttccaacc gtccaggttt tttccattaa agatgttttt cattttttgc
     7021 gaatggagga cggttaccgg gccggtcagg gcaaggatta agatcaggtt ggaaaatttc
     7081 atcgatttaa attacggaat ggttggaaga tgtggaagtt ttcgggctta tcgcatttga
     7141 attgccaaaa ccagactcat gcggttccat aaggatgaac ggcgggcgtg atacgcaaac
     7201 ttgccatctt ttctgagaag actgtcggcg gtgagcccct gaaaattgta tttgaggtgg
     7261 gtggacaggc tgatctttcc cagcaatagg ccataagaca gccccgtgct gaatccaaac
     7321 tgaggaaggg taaagtcata ggggtcaatc aattccgtgt ggtttacggt ttgatcaagg
     7381 tactctgcgg taagtacttt tgttttttga tgcaataaaa tatcgtataa aaatccaaaa
     7441 taaaacgcca tggttttttt atccatgggc agggtatatt tcacatagac cggcaggctc
     7501 aggaaagaga ggtttatcat ggtttcccgg tttaggatct tatttggggg ttgactgaat
     7561 tcaatttccg aactggtcaa taatcttttc cattgtattt ctgtctgaat cgaaattctg
     7621 ttttgtactt tgggagaata aaaattcaat aaaaggacag gaataggatc caggcttctg
     7681 aatttagtgg ggatcaccgt cacggtttcc ccattcctga gttcaaacca ggaataaccg
     7741 gtcccaaatc ccagccccgg gcggattctt agagaaggta agctgttttt gtattcaata
     7801 tagttctcct gtttacactc gttgtaggca atcacttttt ttaaaaggtc ggattcagaa
     7861 aaacgcatgg tggcgatgtc ctcaggacgg tctatacaat ctttcaacac caatcttaat
     7921 atccctttcc aatggatgct ttctgtccct tgcttttctt ccagacggat cagggtatcc
     7981 attgattttt taatataata gacattgtcc gatttgtata aactcaaaaa cccgtcaaca
     8041 atgacttcta aaaacctgcc gcttaaaacg gtggaggaaa atacccggtc attttcaaaa
     8101 ccatagcctg taatttcagt aggacccaat tgtttttttt cacgcccaag tttaaattcg
     8161 caaacatggg tcttttcaac attgatccat gcccggaggg tgtccccgga gttgtacacc
     8221 aaataacctg gtctgaaatc ggcttgcgcc ggcagccggt ccatgcttac aatccaaacc
     8281 gtggagaata aaaacaagag gccagtgccc gggtgatgga acttgcacat agaggttcag
     8341 ctttaagcta atttaggatt atttttatat aaagcaagga aggcgctgtt aaatctctag
     8401 aggaaggggc cgaggctaaa gcatgtaagg gtttttgctg atgcgtattg cacaaatcag
     8461 aatctggatt gctcacctct tttccttatt tttgatgcct ttgaaaaaaa agtcttctcg
     8521 atgaaaatcc ggtttaccct cttggccttt ccggccttta ttttcctgat gctggccgcc
     8581 tgtcgaaagg gtcaggcacc cgcagccgtg gatttgctca ccattaccca ggattcggct
     8641 tctgcccgga agatggcgga ggagataaaa tcccgctcag ccttgaaact ggctcccgga
     8701 cttcaaatcc aggtatgggc taccgactcc ctggccccgg atcccgtggc tatggacatc
     8761 gatgcccaag ggaatattta ccttacgcgg accaaccggc agaaaaattc ggaattcgac
     8821 atccggggat atccccattg gatgacggct tccattgcct tacaatccgt ggaagaccgg
     8881 cgcaagttct tgcatgaaac ctttgccccg gaaaaatccg aagaaaacaa atggctgacc
     8941 gacctcaacc aggacagcat tcacgactgg agggaccttg cggtagaaaa ggaagaagta
     9001 tggaagctgg aagaccgtga ccaggatggt ctggccgagg tttcaacccg ggtgatcaat
     9061 gattttaatg acgaggtttc ggatatcctg ggcggcatcc tggtaagaga gaaggacatg
     9121 tttctcgcca tcgggcccga cctctggcat atggaagaca ccaacgggga cggcgtcatg
     9181 gacaaaaaaa cctcgatctc ccacggatat tcggtgcaca tcggcttcag cgggcacaat
     9241 atgtccggcg tgatcgaagg gccggacggc aagatctact ggaatatcgg cgatatcggc
     9301 gcgagcatca ccgatgtgca gggcaataaa tatcatcatc ccaatgaggg catgatcctg
     9361 cgcagcaacc cggacggcag caattttgaa gtctatgctt ccggtctgag aaatacccat
     9421 gaatttgtat ttgatgaata tggcaacctg atttcgtccg acaatgacgg agaccatcgc
     9481 ggggaacggg aacgactggt ccacatcgtg gaaggctccg atgccggatg gcggtccaac
     9541 tggcaatacg gcaaatacac cgacccgaag aacaacggct ataaagtatg gatggacgaa
     9601 aggctttcgg tcccgcgctg ggaaggccag gctgcttaca tcatccctcc catccagaat
     9661 tttcacaacg gcccgaccgg catggtttac aacccgggta ccgccctcgg ctcggaatgg
     9721 ctaaaaaaat tcttcctggt cgaattcgtg ggcaacccgg cctcctccca tatctgggca
     9781 ttcagcctca agcccaaagg ggcttctttt gacctggata ccgaccttga catggtcagc
     9841 ggattccttc cgaccgggat ccgctttggc gtcgacggtg cactttatgc ggcagactgg
     9901 gtcaatggct ggaataccaa gaactacgga cgggtttgga aaatcgatgt cgccgaagac
     9961 aagaacgatc tgaaagtgca aagagccgaa acacggaaac tgatcggcct taaatacggc
    10021 accctggatg aggcccgtct ggaaactttg ctgaaatacc cggatatgcg ggtacgtctg
    10081 aaagcccagt ttgaactcgc ccgaagagat aaaaaaggat acccccagtt caagaatgcg
    10141 attgcacaga aagaaagtca attggcacgg gtgcatggca tttggggtat cggccagctt
    10201 ttggataaaa ataaaggcta tgctcaagac ctcatcgctc ttttgaccga cccggacgaa
    10261 gagatcattg cccaggcttt gaaagtgctg ggagaccggc ggatcgaaag cgccggggaa
    10321 accatgattc cattcctgaa acatgccaat ccaaggatcc ggttttttgc cgcccagtcc
    10381 ctgggccgga tcaaacacca gctcgcgcag gatgccctga tcgagctgct caggaccaac
    10441 gcagacgaag atgtttatat acggcatgcc tcggtcctgg ccctcacacg cctgggcaat
    10501 gcaagccggc tcgcatccct ggcttcggat gcctcccgcg cggtgaggat cggtggtgtg
    10561 ctggcgctca ggagactgaa aagccccgaa atcagggtct atctgaaaga ccaggatgaa
    10621 tacgttgcca cagaagcggc ccgcggcatc aatgatgacg aatccatccc cgctgctctg
    10681 cctgacctgg ctgaaatggt caatcaaacg gcctttaaat ccgaaccgct tcttcgcagg
    10741 gctatcaatg cctgtctccg tgttggtgaa gaccggcacc tggatatgct gatcgctttt
    10801 gcccagcgct ccgatatttc caatgtcctg aaggccgaag ccatgtcgac tattggcacc
    10861 tgggccaacc ccagtgtatt ggaccgggtg gatggccggt accggggcga aatcaaaaga
    10921 gaggaaacgg tggtcaggca aaaaatcgga cctaaactca aagatttctt atcgtcttcc
    10981 gatcctgata tcctgatcgc cacctcgaaa atgatcgccg aactccagct cgccgaacac
    11041 gcttccgacc tggaatccat attcgtcaaa aacgattctg cggatgtgag ggtggccgcc
    11101 ctcaatgccc tggaacaaat aaaggccacc acacttgccc aactgatccc caaaggcatg
    11161 aaggatccct cgcccaaagt ccgcgcggcc gctttgggta tgctggacaa gatcgaattg
    11221 tccggggccg agctggggcg cacggtgaat gaatttttat ccaaagggag cccgcgcgag
    11281 caacaaaggc tgatgggagt actcggaaaa cttcccatca caaaaacaga atccattcta
    11341 tccggactga tcgaggacct caaagcaaaa aaattaccgg ccgccctggg cctggagttg
    11401 aaagaagcca tagaacaaag cggttcggcc gttttaatgg acaaactcaa agctgtccgt
    11461 tccggcaatg atcccatggg agaatacact ttagccctgg aagggggcaa tccctttgca
    11521 ggctaccgct attttaatga aagcaccacc ggccagtgca tccgttgcca tacggtcaac
    11581 ggcgccggca gcaatgtagg tcccgacctt tcaaagatcg ggtccacact cacgcgggaa
    11641 cagatcttga aatccctggt cgatcccagc gcaagacttg cccccggata cggggtggtg
    11701 gtcctcacct tgaaggacgg ttctgaagtt tacggtaaac tggaccagga aaatgagaag
    11761 gaactggtaa tcatcacctc cgatgcggaa cccctgcgca tcgccacgga acgtatccgg
    11821 gaaagaaaaa acataccctc cagcatgccg gtgatgaccg ataagatgac catgagggag
    11881 atgcgggacg tggtggaatt tttggcgacc cggaagtgag cgggcggtga aaggataaac
    11941 ccagggtgga gctgaccgct gtctaagaaa agtggtcctg ccgataaaat ttaggagaac
    12001 cggctacgga gattctgcga cttaatgata accgttgaat cccgggataa tcatttcgca
    12061 gtaccccggg acaattccat ctttttgcct ttccgcacta tcccgggcac ctccgcgtcc
    12121 tcgtgcgata taaacaggag ggtcctgtct tcgcaaaagg catccaataa atctttcgat
    12181 ttttcaatca tccctaggtc aaaatgctgg aagggctcgt ccaggagcag gaaggccgga
    12241 ttcttcacga tcacccggat gaataaaacc aggcgctgca aaccggtgga gagggcatac
    12301 atgggcgcat cgataaaatc ctccatttgg taatagcgga acaggtcttt gataagggac
    12361 tcggtgtccg gggttagcgt tttgggcgca tagggattgt cccgcaaacc gccggccgcg
    12421 acctgacggg ctgtttgttc ctggtcaaga taaaaatgaa attcgggaga caagaaccca
    12481 atccggtcct tgatgtccca gatgctttcc ccccggccgc gggcatggtc aaacaggatg
    12541 atgtcgttca aataggactg gggatggtcc gcgtagatca ggcttgcgat cagggatttg
    12601 ccggctccgt tgggccccgt caacaaccat ttttctcctc ggcggatggt aaggtttatc
    12661 cggtcgagca gtagtgtttc ttcataacgg atcgtgaccc gatttaaccg gaccgcaata
    12721 tcaaaatcgt tgttttccgg cgggcgaaaa gggatttgga tttttgtctt ttgagcagga
    12781 ttgcccggcc ggttaaacga ttgccgggtg ccggcatagg taattttact ttgttccagt
    12841 tgaaggacat gggtcatgga caccgggacc tgaccatgtg gggcggtcaa aaggacatgg
    12901 atgccggcac cggcaagccg gtcgatcaga tcgttgagtt ccagccgcga ggctttatcc
    12961 aggccggtat aagggctgtc cagaacaagg atttcggggt ttttaagcaa ggctttgata
    13021 atccggactt tccggttttc cccattggac agtttaatga gttccttgtc cagctggtct
    13081 tccacatgaa acatatggag ggatccctcc tgctccctgt cctccattgg tttaccgtca
    13141 aatagataat cccgtacggt cagggtgtcg tcggcatcct ggctgttgta ccgttgctga
    13201 taatagaatt gattggggtc gggggccttg tcctggaaaa aaacaaagcc ggtggcctgc
    13261 ttcagttttt ggaaagcttc catatcctcc gctcccttaa ataaaaacct gccgccggtt
    13321 aatctgaatt caccatgcag ggctttgaag aaagtagatt ttccggagcc gctcggtccg
    13381 gccacgagcc attgctggcc cgctttcagg ctccaggaga agtcggagag catggtctga
    13441 gtacccagac aaaaggtgca gtgagttaat tgaatcagga gcatgggagc acgaatgtaa
    13501 atatagagat tgaggtaggt taagcaagtt tggaaatata ttctcacccc agtcccccac
    13561 ctcagctaaa attacattgc cattaggcgg gcaggcctta ggggagtcgt acgcttggct
    13621 gtcgtacact tgttttattc agctcgccct gtgcatattc aagtaagggc agagcggact
    13681 gtcaggttta agagtgctgc aggaatcttt ggttccccga cgggggcagg ggcaaggaag
    13741 gtttgaggtg ttccc
//
LOCUS       DAOQXT010000116        13724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_4959_length_13724_cov_6.531714, whole genome shotgun sequence.
ACCESSION   DAOQXT010000116 DAOQXT010000000
VERSION     DAOQXT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4959_length_13724_cov_6.531714"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="PKM27_16275"
     CDS             <1..1323
                     /locus_tag="PKM27_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNR08876.1"
                     /translation="DVLRIINEPTAASMAYGLGLQEGSNEIIAVYDLGGGTFDLSILH
                     IQDGVFEVLSTHGDTFLGGDDFDRAIMEYWIDRHGMDKEALLADKSRGQSLRLLAEQS
                     KKHLSLHESFSAVWNEYEFTLTRPEMEKLIDPIIQRTIQSCHQALRDAGLNPGDLSKV
                     IMVGGSTRVPAVTKAVKDLFGDKLYDKLNPDEVVALGAAVQANILSGKDQRFLLIDVT
                     PLSLGIETMGGLMDVIIPRNSKIPSKAGRQYTTSVDGQVNLKISIYQGERELVSHNRK
                     LGEFILRNIPPMPAGLPKIEIQFLINADGILTVRAKELRSGVHSEIEVRSQYGLSEEE
                     MARMLLESVKYARQDMQAKALQEAINEGKSIILATERFILQNEAHLSREETDALREKS
                     KYIDEMIPTGDKDRIIQGIEDLNQYSAPIAHRMLDLNIGQALQGTKAI"
     gene            1324..2382
                     /locus_tag="PKM27_16280"
     CDS             1324..2382
                     /locus_tag="PKM27_16280"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR08877.1"
                     /translation="MNRPAWLPAILFLPLCGLSAQPDPIRREVEKLIRYETNIRNKNL
                     PGFIVAMIEKDTTFVLPFGQRALEDPALVTRDDVFEIGGVSKIFTALLFEKFGEPYHL
                     DINRSINEFLEPDNHSFDSCRLYHLLTHTSGLPRTIPAWGEVESGTENPYAGFDEEDA
                     DHFFSNYLLTQKPDDRYEYSHINYLLLQRILTSVTELDFETLLQETLPPYGIHASCTR
                     ETTLPGYGRDLDARPAWTPDAFRGALGIRASLDDLVRLCRLTLDRETLFASLLLRYPM
                     TVGKEKGWVARGWQVLPIPKNRYVYAHTGRTGGHHVFIGLLPETYTAVVVLSNAAAGT
                     DPLGLSILNMMNRNWKRK"
     gene            2379..2741
                     /locus_tag="PKM27_16285"
     CDS             2379..2741
                     /locus_tag="PKM27_16285"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNR08878.1"
                     /translation="MKKKPLNDLADLRLVFSTKQENPAPEDLPTEVPASTPFAKQNLR
                     IRIDRRHRAGKEVTLLEGLDMNKEEMEALAKKLKGQCGIGGGVDADLIFLQGDQRKKL
                     PGLLQKMGFGKVKVVSNQ"
     gene            3211..4209
                     /locus_tag="PKM27_16290"
     CDS             3211..4209
                     /locus_tag="PKM27_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR08879.1"
                     /translation="MNSYIDLPGITARIQQESGFVDKIREQLAHTLVGQEIMVERLLI
                     GLLCKGHILLEGLPGLAKTLAIKSLANAIHASFQRIQFTPDLLPADIIGTMIFNPGSN
                     EFHVRKGPIFANFILADEINRAPAKVQSALLEAMQEKQVTIGKETYRLEEPFLVLATQ
                     NPIEQEGTYPLPEAQVDRFMLKVLIGYPSMEEERQIIRMNLGDEKSREVRPVVQPGDI
                     LRAREVLNSIYMDEKIEKYILDIVFATRKPEEYKLGQIKHFIQYGASPRASINLALAS
                     KAVAFIQKRGFVIPDDVRYIAKDVLRHRIGLTYEAEAENKNTDHIIGEIISKVEVP"
     gene            4209..5495
                     /locus_tag="PKM27_16295"
     CDS             4209..5495
                     /locus_tag="PKM27_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08880.1"
                     /translation="MKLLFLFHFVPCWMAGQVSQTGVAELFFKDKNLLWEKSYQGVWD
                     DVIPVKLELGYDGTACKGQMFFSDNKARFIVSGTLKDNDLLLEEQDLLGNVTGYLALK
                     ILGDQVRGTWYNAARTFNARIVIREGPPSTTLNYWTRSYALKSAPAETLLIQQKDFSD
                     QIYSRFYYGPLNKTLEGTSPIKHEEDFHQETVLFDPQNQNAGRLDTWRSGERKIEILY
                     KQGKTEWSRTLDLIAQVGMHQESYTDHWMTVDFVFPETDKPGINHWFKSLVDSFLILV
                     QDKKAGQAEQNQTLPANRMAYRLSIWPQVQFLTDQYLSGYVHAKTSWDESIQSQPFLF
                     DLKKGNLVREAELFQNYKSFLEVKELLIEHELRKLRESDGLMYEGLTAGAFTLFTLRK
                     EGLCYSAPFSLQYGFRQVIVPYKDVKDLISPRFISF"
     gene            5501..6376
                     /locus_tag="PKM27_16300"
     CDS             5501..6376
                     /locus_tag="PKM27_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNR08881.1"
                     /translation="MDTTELLQKVRKIEIKTRSISKHLFTGEYHSTFKGRGMSFSEVR
                     PYQYGDDIRNIDWNVTARQHEPFVKIFEEERELTLMLMVDISPSSYFGTHQQLKKEWA
                     TEICAVLAFSAISNNDKVGLILFSDQIELFIPPKKGKQHILRILRELINAEPRGKGTR
                     VAGALQFFNNILKKRSIAFILSDFWDRDYELPLRILANKHDLIGIQLYDRVEQQLPDI
                     GFVQSYDLETGQLQWIDTSSRSLRKEYEERFAKHLADTSEIFRKSSSDLIQLRTDHSY
                     IEALMAFFKKRAKAA"
     gene            6373..7299
                     /locus_tag="PKM27_16305"
     CDS             6373..7299
                     /locus_tag="PKM27_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08882.1"
                     /translation="MKKALLTGLLSVILACGWAQVDFISRVDSTSMLLGDQQNLVLRL
                     SSPAGVPPADIHTEALDTCTFLEIVHAGTWTRTEENQQVIQQKTIRFTIFDEGQYLIP
                     PCYAVLGSDTFRSYPIPMIVAGIEPDTTGLLPIKGIIREQACWTDYLAWILVLVTLLF
                     GYLAWRYYKNRRAKKTFEAAPVPEIQLLPHEIALQKLQVLKQNRIWEKGEVKEFHVQL
                     THILREYLEGRYLVPALESTSIEIIRDLRAKGLGQEMVQKANDLLHVADWVKFARGNP
                     EENANALALEHCLEWVRQTAPSEPEPLAPKPH"
     gene            7303..8307
                     /locus_tag="PKM27_16310"
     CDS             7303..8307
                     /locus_tag="PKM27_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNR08883.1"
                     /translation="MQALSSFRFVYPWLLVLLVLVPLVWWLYRRASRNPTRAVRFPAV
                     IKPSRTYWRNVVSDFLPWMRLLALALICLALARPQRIWKEEKIKANAIDIFMVIDLSS
                     SMLAQDFQPDRLSVSKQVAENFIDKRKYDRLGLSVFAGESFTRCPLTTDHAVLKNFLK
                     NLNVGNLEDGTAIGMGLSAAVNRLKNSTSKSKIIILLTDGVNNSGYIDPLTAAQLARE
                     FQIKIYTVGVGSMGEALTPVTRRGDGQYVFAMARVEIDEQLMTEVSEMTQGKYYRATD
                     EEALQKIYDEIDKLEKTEIEVTRFTRYTEIFHYFLGSALALLLLEFLVRNFVIRQLID
                     "
     gene            8309..9337
                     /locus_tag="PKM27_16315"
     CDS             8309..9337
                     /locus_tag="PKM27_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNR08884.1"
                     /translation="MFRFEHPGFLYLCGLVLVLAGLRYYLRKQQSHRLKKLAGESLWP
                     TLVPGFNPKTEALRSMLWLGGLFLIILALSNPQWGVRKEKVEIKSTDIYLALDISNSM
                     LAEDIKPSRLERAKLWSESLIMSLAGDRIGTILFAGNAFLQSPLTQDYGSATMFIRSA
                     YPDLISTQGTNLQDAIQTGLDHFSEDQEAQRVILILSDGEDHEESRQAALAAAAKARE
                     KNVALFTIGVGTESGGMIPVKTNQLEDYKRDETGKPIISRLQESVLKELAEASRGKYY
                     SMRDGDAIIPAIKNELANMEKQRLAERSYSEFESYFPYFLLPGLILLMLEFLIGIRWF
                     DRFKKPAL"
     gene            9334..10014
                     /locus_tag="PKM27_16320"
     CDS             9334..10014
                     /locus_tag="PKM27_16320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR08885.1"
                     /translation="MRMFLFMALSLAGICTRGQSNHQLLKRGDQAYLKQDFNRAEENY
                     RRALENKKDEKTTYNLGNAVIEQNRPDEAIPLYEDAAKKQNDPTLRARAWHNLGNAFY
                     LKKDYQNSVNAYKEALKVKPDDLDTKKNLVLAKKQLLKQQQQQQQQQNQDRQDNQDQQ
                     DQQPNPQGEDQQGQQQQAKEKMPSQPKEMSREEAEQLLQFTEREDQRVQKKLRNKNDK
                     TPPPKKDW"
     gene            10044..10502
                     /locus_tag="PKM27_16325"
     CDS             10044..10502
                     /locus_tag="PKM27_16325"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNR08886.1"
                     /translation="MKYPIFFLLSLVAGQLTGQAGDVRVSVSRDTVLLGNAFFVQYTI
                     NRNDLRFRTPEFQDGHLLSQSSTSNTSIQDGKVQFQSTHRFLIQPERAGVFTIPSISL
                     PGTTIPAVEIYVQDNPQGLLEDPETDMEWSKRDLFHDSPVKNPVRKTRKL"
     gene            10499..12208
                     /locus_tag="PKM27_16330"
     CDS             10499..12208
                     /locus_tag="PKM27_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNR08887.1"
                     /translation="MRKGLACILWVLSSGWSFAQITFTARTDQAEITQDQYLELAFVL
                     ENAQGSQFSPPRLDDWTVISGPSTSSQMTIVNGTRSSSFSYIYSLAPKRLGTLTIGSA
                     SIQVSGKTFSTDPVTIRVVKAAPSNNQGINRTPGKGDLFIQVTAKPEKAFVGQQIQVE
                     YKLFTTLDVDNYNISYTPDFKGFHLSHVPRFNFPTNRETLNGRTYMTKVIQAFAVYPI
                     QSGDYAIDPMHVRVSIVVDEDNAQSFFLLPTTKGINLMSNPLDIRVEALPAPVPAHFS
                     GAVGRFKMASRIDQLAIKTNEAAMITIYLEGDGDLNRVGKPFLQTDSSVFQVYEPKVT
                     REDTDYKVGGFISQREFSYPLVALAPGNYKIIPSFTYFDVDSNAYLTLSPEVFNMLVT
                     GQAVTSAPEPAGPAGPGGEILPLIANPAMHRGNQFVGSAGFFLLLLLPGLILLGQDIL
                     LRGRRRRERLGKSERLKLQARQQALQKLDSLLSSEGPKRMEEVNALLTGYLRTKWGLG
                     EQPYTLQEWHGIVDAQVIPPESKKEIHGLLHAFELAIYAGQPTADQFNEWVMKAKRVV
                     EGI"
     gene            12345..13070
                     /locus_tag="PKM27_16335"
     CDS             12345..13070
                     /locus_tag="PKM27_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNR08888.1"
                     /translation="MSGAAEKKEEKLGVMNLLILVLSIYVLLVLVIDTVFKLPEEVSQ
                     LLTYIDNVICLVFLYDFSLRFYRAENKLKFMRWGWIDLISSIPTLDYFRAGRALRLVR
                     LLRILRAYRSTKHLVEHIFQSRTQGAFSTVMIVAIIMLIFSSIAILQVETDPNSNIKN
                     AEDALWWAFVTITTVGYGDKFPVTTEGRVIAIALMMVGVGLFGTYSGFIASWFMDATK
                     KKQEEEDKKHEEEDRKEAEGEKS"
BASE COUNT         3491 a         3766 c         3437 g         3030 t
ORIGIN      
        1 gatgtgctgc ggatcatcaa tgaacctacg gccgcaagta tggcctatgg actgggcctg
       61 caggaaggct ccaatgaaat catcgcggtt tatgacctgg gcggagggac ctttgacctg
      121 tccattcttc atatccagga tggggtcttc gaggttttat ccacccatgg ggacaccttc
      181 ctcggcgggg acgattttga ccgcgccatc atggaatact ggatcgaccg gcatgggatg
      241 gacaaagaag ccctgctcgc cgataagagc cggggtcaat ccctccgcct cctagcagaa
      301 caaagcaaaa aacacctgtc cctgcatgaa tccttttccg cagtatggaa tgaatatgaa
      361 tttaccctta cccggccgga aatggaaaaa ctcatcgacc cgattattca aaggaccatc
      421 caatcctgcc atcaggccct ccgggacgcg ggtctgaacc ccggagactt aagcaaggtg
      481 atcatggtag gcggctccac ccgcgtgcct gcggtcacca aggcagtaaa ggatttgttc
      541 ggagataaac tttacgataa actcaacccg gacgaggtgg ttgcccttgg agccgccgtg
      601 caggccaata tcctttccgg gaaagaccag cgtttcctcc tgatcgatgt cacgcccctg
      661 tccctgggga tagagaccat gggcggattg atggatgtga tcattcccag aaattcaaaa
      721 atcccttcga aggccggcag gcaatacacc acctcggtgg acggtcaggt caatctaaaa
      781 atctcgattt accagggcga aagggaactg gtatctcaca accggaaatt gggcgagttc
      841 atccttagaa atattccgcc catgcccgcg ggccttccga aaatagaaat tcaattcctg
      901 atcaacgccg acgggattct gacggtcagg gccaaagagc tccgctccgg tgtgcacagc
      961 gaaattgaag tccggtccca atacggattg tcggaggaag aaatggcccg gatgcttctg
     1021 gaatcggtaa aatatgcccg ccaggacatg caggcaaaag ccctgcagga agccatcaat
     1081 gaaggaaaat ccataatcct ggccacggaa cggtttatcc ttcaaaacga agcccacctc
     1141 agccgggagg aaaccgatgc cctgcgggaa aaaagcaaat acattgatga aatgattccc
     1201 accggggaca aagaccggat catccagggc atcgaggacc tcaatcaata cagcgccccc
     1261 attgcccacc ggatgcttga cctcaacatc ggccaggccc tgcagggaac caaagccatt
     1321 taaatgaacc gccccgcctg gctgccggca atcctttttc ttcccctttg cggcctttcg
     1381 gcccagccgg accccatccg cagggaagtc gaaaaactca tccgttatga aacgaacatc
     1441 cggaacaaaa acctgcccgg cttcatcgta gccatgatcg aaaaagacac cacttttgtc
     1501 cttccctttg gccaaagggc gctggaagat cccgctctgg tcacccggga cgacgtcttt
     1561 gaaatcggag gcgtttccaa aatctttacc gccctgttgt ttgaaaaatt tggagagccc
     1621 tatcacctgg acatcaaccg gtccatcaat gaattcctgg aacccgacaa ccactctttt
     1681 gattcctgca ggctctacca tctgctcacc catacctccg gcttgccccg gaccattccg
     1741 gcctgggggg aggtggaatc gggtacggaa aatccctatg ccggatttga cgaggaggat
     1801 gccgatcatt ttttttcaaa ttatttattg acccaaaaac cggacgaccg atacgaatac
     1861 agccatatca actatcttct tttacagagg atcctgacgt cggttacgga actggatttt
     1921 gaaaccctgc tgcaggaaac cctcccgccc tacggcatcc atgcctcctg cacccgggag
     1981 accacgctcc ccgggtatgg ccgcgacctg gacgccaggc cggcctggac gccggatgcc
     2041 ttccgcgggg ccttgggcat ccgggccagc ctggacgacc tggtccgctt gtgcaggctg
     2101 accctggacc gggaaaccct gtttgcttcc ctgttgttgc gatatccgat gaccgtagga
     2161 aaagaaaaag gctgggtagc cagaggctgg caggtcctgc ccataccgaa aaaccgttat
     2221 gtttacgccc ataccggccg cacgggcggg caccacgttt ttatcggatt gttgcccgaa
     2281 acgtatacgg ccgtggtggt gctctccaat gccgcggccg gcaccgaccc gcttggcctg
     2341 agcatcctga acatgatgaa ccgaaactgg aaaagaaaat gaaaaagaaa ccgctgaatg
     2401 accttgccga cctccgcctg gtcttctcca ccaagcagga aaatccggcc ccggaagatc
     2461 ttcccaccga agtccccgcc tccactccct ttgcaaaaca aaacctgcgc attcgcatcg
     2521 accgcaggca ccgggcaggc aaagaagtca ccctgctgga aggcctggat atgaacaagg
     2581 aagaaatgga agccctggct aaaaaactga aaggtcaatg cggcatcggc ggcggtgtgg
     2641 atgcagatct tatttttctg caaggggatc agcggaaaaa actcccgggg ttactgcaga
     2701 agatgggatt tgggaaggtt aaagtagtca gtaatcagta gtcagtagtc agttagcaag
     2761 ttggcaagtt ggcaagttgg caagttggca agttggcaag ttggcaagtt agtgtgctcg
     2821 cagctcgttt ctcgcttctc gttgctcgca tctcgtttct cgcttctcgt tgctcgcatc
     2881 tcgtttctcg cttcttatgt gcggtcgact ccccgtgggg atggggtgtg cggatgaaat
     2941 cagtaatcag tagtcagtag tcagttagca agttggcaag ttagtctgct cgagctcgaa
     3001 gctcgtttct cgtttctcgc ttctcgcttc tcgtttctcg catctcgttt ctcgcttctc
     3061 gtttctcgtt tctcgcttct cgcttctcgt ttctcgcatc tcgcttctcg ttgctcctta
     3121 acgtctggtt aaacccgctg aaaaagcagg gcatgcccgt gacatccaat aaaaattaac
     3181 gatttttgcc cggcttcaac tactcaattt atgaacagtt atatcgattt gccgggcatc
     3241 accgcccgca tccagcagga atcgggcttt gtggataaga tcagggaaca attggcccat
     3301 actctcgtgg gccaggaaat catggtagaa cgcctgctga tcggtctgct ctgcaagggg
     3361 catattctcc tggagggcct tccgggcctg gccaaaaccc tggccatcaa atcgttggcc
     3421 aatgccatcc atgccagttt ccaacgcatt cagtttaccc ctgacctgct gccggccgat
     3481 atcatcggga ccatgatctt taacccgggc tccaatgaat tccatgtgcg caagggcccc
     3541 atttttgcca actttatcct ggcagacgag atcaaccgcg ccccggccaa ggtacagagc
     3601 gcgctgcttg aagccatgca ggaaaagcag gtgactattg ggaaagaaac ctaccgcctg
     3661 gaggagccct tcctggtcct ggccacccaa aacccgatcg aacaggaagg aacctacccc
     3721 ttgcccgaag cccaggtgga ccgttttatg ctcaaggtcc tcatcggtta tccctccatg
     3781 gaagaggaaa ggcagattat ccgtatgaac ctgggcgatg aaaaatcaag ggaggtccgg
     3841 ccggtggttc agccggggga tatcctgcgc gccagggaag tcctgaattc catctatatg
     3901 gacgaaaaaa tcgaaaaata catcctcgac atcgtgttcg ccacccgcaa gccggaggaa
     3961 tacaaactcg gccagatcaa acattttatc cagtatgggg cctccccgcg cgccagcatc
     4021 aacctagccc tcgcatccaa agcggtggcc ttcattcaaa aaagaggatt tgtcatcccg
     4081 gacgatgtgc gttatatcgc aaaggatgtc ctgcgtcacc ggatcgggct cacttacgaa
     4141 gcagaagcag aaaataaaaa tacggaccac atcatcggtg aaatcatttc aaaagtagaa
     4201 gtaccctgat gaaattgtta ttcctgttcc actttgtacc ctgctggatg gctggacagg
     4261 tcagccagac cggggtcgcg gagctttttt ttaaggacaa aaaccttctc tgggaaaaat
     4321 cgtaccaggg ggtatgggac gatgtcattc ccgtaaagct ggaactggga tacgacggca
     4381 cggcctgcaa agggcaaatg tttttcagcg acaacaaagc ccggttcatt gtttccggca
     4441 ccctcaaaga caacgatttg cttctggagg agcaggatct cctgggaaat gtcaccggat
     4501 acctcgccct gaagatcctg ggagaccagg tccggggaac ctggtacaac gcggcgcgga
     4561 ccttcaatgc ccggatagtc atccgcgagg gccctccgtc caccaccctg aattactgga
     4621 ccaggtccta tgccctcaaa tccgccccgg ctgaaacctt gctcatccag cagaaggatt
     4681 tcagcgacca gatctattcc cggttttatt acgggccgct gaacaaaacc ctcgagggga
     4741 ccagcccgat aaaacacgaa gaggacttcc accaggaaac ggtattgttt gatccccaaa
     4801 atcagaatgc cggccggctc gatacctgga gatcaggcga acgtaaaatc gaaatcctgt
     4861 acaaacaggg aaaaacggaa tggtccagga ccctggatct catcgcccag gtcggcatgc
     4921 accaggaatc ctatacggac cattggatga ccgtcgattt tgtttttccc gaaacggaca
     4981 aaccgggcat caaccattgg ttcaaatctt tggtcgactc cttcctgatc ctggttcagg
     5041 ataaaaaagc cgggcaggcg gaacaaaatc aaaccctgcc ggccaaccgc atggcttacc
     5101 ggctgagcat ctggccccag gtccagttcc tcaccgacca gtatttgtcg ggttatgttc
     5161 atgccaaaac ttcctgggat gaatccatac aatcccagcc ttttcttttt gacctcaaaa
     5221 aagggaacct ggtccgggaa gccgaacttt ttcagaatta taaatctttc cttgaagtaa
     5281 aagaactgct gatcgagcac gaattgagaa aactccgcga atccgacggc ctaatgtacg
     5341 agggcctgac cgcgggggca ttcaccttgt tcaccctgag aaaagaggga ctttgttatt
     5401 ccgccccatt cagcctgcag tatgggttca ggcaggtgat cgtcccttat aaggatgtaa
     5461 aggacctgat ttccccacga tttatttcat tctgaacctt atggatacca ccgaacttct
     5521 ccaaaaagtc aggaagatag agatcaagac ccggagtatc agcaaacatt tgttcaccgg
     5581 ggaataccac agcaccttta agggccgcgg catgtctttc agcgaagtgc gcccttacca
     5641 atacggggac gatatccgca acattgactg gaatgtgacc gcccggcagc atgaaccctt
     5701 tgtcaagatc tttgaagaag aaagggaact caccctgatg ctgatggtgg acatcagccc
     5761 ctcttcttat tttggcaccc atcagcaact gaaaaaggaa tgggcaaccg agatctgcgc
     5821 cgtcctggcc ttttcggcca tcagcaacaa cgataaagta gggctcatcc tgttttccga
     5881 ccaaatcgaa ctttttattc ccccaaaaaa agggaaacaa catatcctgc ggatcctccg
     5941 cgaactcatc aatgccgaac ccagggggaa aggcacccgg gtagcgggcg cccttcagtt
     6001 tttcaacaac attttgaaaa aaagaagcat tgccttcatc ctttccgatt tctgggaccg
     6061 cgattacgaa ttgcccctcc gcatcctggc taacaagcac gatctgatcg gcatccagct
     6121 ttatgaccgg gtggaacaac aattgccgga tatcggcttc gtccagtcct atgacctgga
     6181 aaccggtcaa ctgcaatgga tcgatacctc cagccggtcg ctgaggaagg agtacgaaga
     6241 acgttttgcc aaacacctcg cggacaccag cgaaatattc cgtaaaagtt cgtcggacct
     6301 catccagctt cgcacggatc attcctatat cgaagccctg atggcatttt tcaaaaaaag
     6361 ggccaaagcc gcatgaaaaa ggccctgctg accggactcc tttccgttat actggcctgc
     6421 ggctgggccc aggttgattt tatttcacgg gtggacagca cttcgatgtt gcttggcgac
     6481 cagcaaaacc tggtattaag gctgagcagt cctgcggggg tgcctccggc ggacatccat
     6541 actgaagccc tggatacctg cactttcctg gagattgtac atgcaggcac ttggacccgg
     6601 actgaggaaa atcaacaggt aattcaacaa aaaaccatcc ggttcaccat cttcgatgag
     6661 gggcaatacc ttattcctcc ctgttacgcg gtccttggtt cggatacctt tcgcagctat
     6721 cccatcccca tgatcgtggc cggcattgaa ccggatacaa cagggctcct gcccatcaaa
     6781 ggcattatca gggaacaggc atgctggacc gattacctgg cctggatcct ggtcctggtt
     6841 accctgctgt ttgggtacct ggcctggcgc tattataaaa accggagggc gaaaaaaacg
     6901 tttgaagccg cccccgttcc ggaaatccaa ctcctccccc acgaaatcgc cctgcaaaaa
     6961 ctccaggtcc tgaaacaaaa ccgcatttgg gaaaaggggg aagtgaagga attccacgtt
     7021 cagctcaccc atatcctgcg tgaatacctg gaaggccgct acctggtccc tgctttggaa
     7081 tccaccagca tagagatcat ccgggacctt cgcgcaaaag gtcttggcca ggagatggtg
     7141 caaaaagcca atgacctgct gcacgtagcc gactgggtta aatttgcccg cggaaatccg
     7201 gaggagaacg ccaatgccct cgcccttgaa cattgcctgg aatgggtgag acagaccgcc
     7261 ccgtctgaac ccgaacccct tgccccaaaa ccccattgaa ccatgcaggc cttatccagt
     7321 ttccgttttg tatatccctg gctgctagtc ctgcttgtcc tcgtcccatt ggtttggtgg
     7381 ctgtaccgcc gggcgtctag gaatcccacc cgggcggtcc gattccctgc ggtgatcaaa
     7441 ccctccagga cctattggag aaatgtcgta tccgacttcc tgccctggat gcggttgttg
     7501 gcgctggcgc tgatctgtct cgccctggcc cgcccccagc gaatctggaa ggaagaaaaa
     7561 ataaaggcca acgcgattga tatttttatg gtcatcgact tgtccagctc catgctggcc
     7621 caggattttc agccggaccg gttgagtgta agcaaacagg tggccgaaaa cttcattgac
     7681 aaaagaaaat acgaccggct cggtctgtcc gtctttgccg gcgaaagttt tacccgctgc
     7741 cccctgacta cggaccatgc ggtccttaaa aatttcctga aaaacctgaa tgtgggcaac
     7801 ctcgaggacg ggacggccat cggcatgggc ctctccgctg cagtaaaccg gctgaaaaac
     7861 agcacttcaa agagcaagat catcatattg ctgacggatg gggtcaacaa ttcaggatac
     7921 atcgatcctc tcaccgccgc ccaattggct agggaattcc agatcaagat ctataccgtg
     7981 ggcgtaggca gcatgggcga ggccctcacc ccggtgaccc gaaggggaga cggccaatac
     8041 gtctttgcca tggcccgggt ggaaatcgac gaacaactca tgaccgaagt cagtgaaatg
     8101 acccagggca aatactaccg cgccaccgac gaagaggccc tgcaaaaaat atacgatgaa
     8161 atcgacaagt tggaaaaaac cgaaatcgaa gtcacacggt ttacgagata tacggagatt
     8221 ttccactatt tcctgggatc ggccctggcc ctgttgttat tggaatttct ggtccgcaat
     8281 tttgtgatca ggcaattgat cgattgatat gtttagattt gaacatcccg gtttccttta
     8341 tttatgcggc ctggtcctgg tccttgccgg cttgcggtat tacctcagga aacaacaatc
     8401 ccaccgcctg aaaaaactgg ccggggaatc gttatggccc accctggtgc cgggctttaa
     8461 tcctaaaacc gaggccttgc gttcgatgct ctggctggga ggcctcttcc tgatcatcct
     8521 ggccctgagc aatccccagt ggggggtgcg caaggaaaaa gtggaaatca aatccaccga
     8581 catctacctt gccctggaca tttccaacag catgcttgcc gaagacatca aacccagccg
     8641 cctcgaaagg gccaaactgt ggtcggagtc cctgatcatg agcctggccg gggaccgcat
     8701 cgggaccatt ctctttgccg gcaatgcttt tttgcaatct cctcttaccc aggattacgg
     8761 atcggccacc atgtttatac gaagcgccta ccccgacctg atttccaccc agggcaccaa
     8821 tttgcaggac gcgatccaga ccggcctcga ccatttttca gaagaccagg aagcccagcg
     8881 cgtcatcctg atcctttccg acggagagga tcacgaagaa agcaggcagg ccgccctggc
     8941 cgccgccgcg aaagcccgcg aaaaaaacgt cgcccttttt accattgggg tagggacgga
     9001 atccggagga atgatcccgg taaaaaccaa ccagttggaa gactataaaa gggatgaaac
     9061 cggcaaaccc atcatcagca ggctccagga atccgttttg aaggagctgg ccgaagcgtc
     9121 gaggggcaaa tattattcca tgcgcgacgg ggatgcgatc atcccggcga tcaaaaacga
     9181 actggcgaat atggaaaaac agcgcctggc ggaaagaagt tattctgaat tcgagagtta
     9241 tttcccctat tttttattac cgggactgat tctgctgatg cttgaatttc tgataggcat
     9301 ccgctggttc gaccggttta aaaaacctgc cttatgagaa tgtttttatt tatggccctg
     9361 agtcttgcgg gaatttgcac ccggggccaa tccaatcatc aattgctcaa aagaggcgat
     9421 caagcctact tgaaacagga ctttaaccgt gcggaggaaa actaccgcag ggccctcgaa
     9481 aataaaaagg atgaaaagac aacctacaac ctgggcaatg ccgtgataga acaaaaccgg
     9541 cccgacgaag cgatccccct ctatgaagac gcagctaaga aacagaacga tcccaccctg
     9601 cgcgcgcggg cctggcacaa cctgggaaac gctttttacc tgaagaaaga ttatcaaaac
     9661 agcgtgaacg cctataagga ggccttaaag gtcaaaccgg acgacctcga taccaaaaaa
     9721 aacctggtgc ttgctaaaaa acaattgttg aaacaacagc aacaacagca gcagcaacaa
     9781 aaccaggacc ggcaggacaa ccaggaccag caggaccaac aacccaatcc ccagggagag
     9841 gaccagcagg gccagcaaca acaggcaaaa gaaaaaatgc cttcccagcc caaggaaatg
     9901 agcagggagg aagcggaaca attattgcaa tttacggaac gggaggatca gcgggttcag
     9961 aagaaactac gcaataaaaa cgacaaaacg cctcccccca aaaaagactg gtgagtaatt
    10021 ttgcgaacaa aaatgtgagg gccatgaaat acccgatttt ttttctttta agccttgtcg
    10081 ccggccagtt gaccgggcag gccggggacg tgcgggtatc cgtcagccgg gataccgtcc
    10141 tccttggaaa tgcctttttt gtacagtaca ccatcaaccg caacgatctc cggttccgca
    10201 cgcctgagtt tcaggacgga cacctcctca gtcaaagctc gacgagcaac acctccattc
    10261 aggatggaaa agtgcagttc cagtccaccc atcgctttct gatccagccg gaaagggcgg
    10321 gtgtgtttac catcccgtcc atttccctcc cggggaccac catcccggcc gttgaaatct
    10381 atgtgcagga caatccccag ggtttgttgg aagaccctga aaccgacatg gaatggtcca
    10441 aacgcgatct gttccatgac tcccctgtga aaaatccggt gcggaaaacc cgtaaattat
    10501 gagaaagggc cttgcctgta ttttatgggt tctttcctca ggctggtctt tcgcccagat
    10561 tacctttacc gcccgaaccg accaggcgga gatcacccag gaccaatacc tggaactggc
    10621 ctttgtcctc gaaaacgccc agggcagtca gttcagccct ccgaggctgg acgattggac
    10681 cgtcatcagc ggaccaagca cgtcttccca aatgaccatt gtaaacggga cccggagttc
    10741 gagtttttct tatatctatt ctctggcccc caagcggctg ggaacgctga ccattggttc
    10801 cgccagcatc caggtatccg gcaagacctt cagcaccgat cccgtcacca tccgcgtggt
    10861 gaaagcggct ccttccaaca accagggcat caaccggacc ccgggaaagg gagacctctt
    10921 tatccaggtt accgccaagc cagaaaaggc cttcgtaggt cagcaaatcc aggtggaata
    10981 caaactgttt accaccctgg atgtggacaa ttacaacatc agttataccc ccgattttaa
    11041 gggcttccac ctcagtcatg tcccccggtt taattttccc accaaccggg aaacgctgaa
    11101 tggtaggact tatatgacca aagtgatcca ggcctttgcg gtctatccga ttcagagcgg
    11161 cgactatgct atagatccta tgcacgtgag ggtcagcatc gtggtggatg aagataatgc
    11221 ccagtccttt ttccttctgc ccacgaccaa agggatcaac ctgatgagca acccgctgga
    11281 tatccgcgtg gaggccctgc ccgcgcccgt tcccgcccat ttttccggtg cggtcgggcg
    11341 attcaaaatg gcttcccgga tcgatcagct tgctattaaa accaatgaag cggcgatgat
    11401 caccatttac ctggaagggg acggtgacct gaaccgggtg ggcaagcctt ttttgcagac
    11461 cgattcctcg gtattccagg tctatgagcc caaggtaacc cgggaggata ccgactacaa
    11521 agtgggcgga ttcatcagcc agcgtgaatt ttcctatccc ctggtggccc ttgcgccggg
    11581 caattataaa attataccct cctttaccta ttttgatgtg gacagcaatg cctacctcac
    11641 cttgtccccg gaggtattca atatgctggt caccggacaa gccgtgacct ccgctcccga
    11701 accagccggg cctgccgggc caggaggcga gatcctgccc ctcatcgcca atccggcgat
    11761 gcatcggggc aaccagtttg taggttcagc gggctttttc ctgcttttac ttttgcccgg
    11821 gctgatcctt ttaggccagg atattttact tagagggcgt aggcgacggg aacgcctggg
    11881 caaatccgaa cgcctgaaac tccaggccag gcaacaagcg cttcagaaac tggacagcct
    11941 gctctccagt gaagggccca agcggatgga ggaagtgaat gcactgctga ccggatacct
    12001 ccgcaccaaa tggggccttg gagaacaacc ctataccctg caggaatggc atggcattgt
    12061 agatgcacaa gtcatccccc ctgaatctaa aaaggagatc catggcctgc tccatgcatt
    12121 tgaactggcc atctatgccg ggcagccaac ggccgaccag tttaatgaat gggtgatgaa
    12181 ggcgaagagg gttgtagagg gaatatgacg cgagatgcga gatgccagac gcgagatgcg
    12241 agactaataa actgataaac ttgctaactt gctaacttgc taacttgcca acttactaac
    12301 ttactaactt actaacttac caacttacta actaactttc acccatgtcc ggagcagcag
    12361 aaaaaaaaga ggaaaaactg ggggtcatga atctgctgat cctggtactt tctatatatg
    12421 tgctgttggt gctcgtgatc gacacggttt tcaaattacc agaggaggtg tcccagctgc
    12481 tgacatatat cgacaatgtg atctgcctgg tctttttata tgatttttca ctacgctttt
    12541 acagggcgga aaacaagctg aaattcatgc gatggggatg gatcgacctc atttccagca
    12601 tccccaccct ggattatttc cgggccgggc gagccctccg gttggtacgc ctgctgcgca
    12661 tcctcagggc ttaccggtcc accaaacacc tggtggaaca catctttcag tccaggaccc
    12721 agggggcttt ctccaccgtg atgatcgtgg ccatcatcat gttgatcttt tcctccattg
    12781 ccatcctcca ggtcgagacc gatcccaaca gcaatatcaa aaacgcggaa gacgccctct
    12841 ggtgggcttt cgtgaccatc accacggtgg gatatggcga caaattcccc gtcaccacgg
    12901 aaggaagggt gatcgccatc gccctgatga tggtcggcgt gggattgttc ggaacctatt
    12961 ccggtttcat cgcctcctgg tttatggatg ccacgaagaa gaagcaggaa gaggaagata
    13021 agaaacatga ggaagaggac cggaaggaag cggaaggaga aaaaagttga atcgtggttt
    13081 ataagccacg gattattgtc ttaccaccac atttattcat agttcgaacg gaggccacaa
    13141 aggtcacaca tattttgagt tagagttatt gaagtttcag aggagtttta atactggaaa
    13201 aaaactcgct tccgtgctct ccagatcccc gtgtgccttc ttgggtaggt ttatgatgcc
    13261 ttcggcatca taaatgtgtt tatggttcgc acggagttcc cggagggcac ggagatatta
    13321 agttagagta tttgaagttt cagaggagtt ttaatatggg aaaaaaactc gcctccgtgc
    13381 tctccagatc cccgtgtgcc ttcttgggta ggtttatgat gccttcggca tcataaatgt
    13441 gtttatggtt cgcacggagt tcccggaggg cacggagata ttaagttaga gtatttgaag
    13501 tttcagagga gttttaatat gggaaaaaaa ctcgcctccg tgctctccag atccccgtgt
    13561 gccttcttgg gtagttttta tgatgccttc ggcatcataa atgtgtttat ggttcgcacg
    13621 aagttcgcgg agggcacgga gatattaagt tggagtattt gagtttcaga gtaagcaccc
    13681 ggccaaccac atattgatca aggcaagagt ggggcataga ttta
//
LOCUS       DAOQXT010000117        13369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5120_length_13369_cov_6.635797, whole genome shotgun sequence.
ACCESSION   DAOQXT010000117 DAOQXT010000000
VERSION     DAOQXT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5120_length_13369_cov_6.635797"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PKM27_16340"
     CDS             complement(<1..1221)
                     /locus_tag="PKM27_16340"
                     /inference="COORDINATES: protein motif:HMM:NF027309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HNR08889.1"
                     /translation="MYRGYRLCRAIQAFIMLIFLNPLFAQSWLSSESGFPLHQAGAIV
                     IDPGDEALTLKTAELFRQDLEMVFGIHPEIISDADPARLAGIKNPVLLGTIAGSSLVK
                     AWAAQQHINLESLQGKWETYLVSVVDQSGSGQKSLLLAGSDRRGMAFGAFELCKQMGV
                     SPWYWWADVPVVKKEALYVKDKLLVTDAPKVKYRGIFINDEAPALSGWTREKFGGFNH
                     LFYQKVFELILRLKGNYLWPAMWGNAFYDDDFMNRAYADLYGIVIGTSHHEPLMRAHD
                     EWRRYGKRQKWNYDSTEAGLKEFWKGGMQRANHEKIVSIGMRGDGDEPMSRETATALL
                     ERIVRDQRQIIEEVTGKPAPETPQLWALYKEVQDYYDQGMRVPDDVTLLLCDDNWGNI
                     RRLPKTGDAPRKGGY"
     gene            complement(1251..3323)
                     /locus_tag="PKM27_16345"
     CDS             complement(1251..3323)
                     /locus_tag="PKM27_16345"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNR08890.1"
                     /translation="MIKNLGAWGIRITKAKGLCFLFIFPFFLFTHCKGSKNKKPLEGS
                     TSPYLLQHLDNPVLWHPWNEGALELAKKLDRPILISMGYASCHWCHVMEEESFMDTAV
                     ARLMNDHFICIKIDREERPDIDQLYMHALQLIQGSGGWPANVFALPDGRPFHAVTYME
                     KNQWTDLLSQITALYRAQPGKIRQQALLLEQDIRADPLVRLPAPADTAFRTGLYQALA
                     DSLIRHSDPDYGGLEGEPKFPMPSVWLTLLEYQSWKKDSTALEQVVRTLDAMASGGIY
                     DQLGGGFSRYSTDRFWQWPHFEKMLYDNAQLISLYSEAFQVTGKPGYAKIVRESLHFM
                     EKELKAPAGGYYSSVNADSEGEEGKYYLWTRKALRDLLSPEEFALFEKAYRISQDENK
                     DNVLYLDGAENFSASQKEMLESCRAKLVAARERRIAPATDTKIITSWNALAIGACLDA
                     FHALGDSLFLGQALQTASFLLQTQLKPDGRLLRTGSNYGFLDDYALAIKSLLALYETT
                     FDRQWLQQSDRLMKRVIVDFSGQNSALFYYSPEETSLFTRRKDHYDEALPSSNAVMAR
                     NLYKMGTLLQDSGYLSRAEAMVKEVLPGILAYPSSFSHWISLYGWIACGSHEIVILGD
                     QAPRVNQEMRRHYLPFTVYMGGREESLPLTKGKYKEGETTIYVCRDKVCKLPVSTALE
                     ALDQIESR"
     gene            complement(3320..6442)
                     /locus_tag="PKM27_16350"
     CDS             complement(3320..6442)
                     /locus_tag="PKM27_16350"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR08891.1"
                     /translation="MPQMFYILLAFIPFGLRSQNPAYFLSNPSLTPDGETVVFCFEGD
                     LWKADVQDGMATRLTAMQGYESSPRVSPDGQWIAFTGRQYGNGDVFVIPLKGGEVRTL
                     THHSANEEMRSWSWDSKTIYFNSYITGKVASHQVSAEGGTPVRVFGDQFFLYDHNLAE
                     HPVTGELFFNDTWESDNQLARKRYKGPFNPDIQSYHPKTKQYKKYTTWEGKDFGTTID
                     RKGNIYYISDQANGEYNLYTLNRNTPKALTSFSTSIKSPQVNAQGGRVVFEKDYQLWI
                     YEVRSGKARPLAISIHRNPILPKEKDFELKGQISAYDLSPDGKKMALVSRGELFVSDA
                     EGKFIRHLKRPTSERVKEVKWLSNNKTLLFNQTLDGYSNWFTMPADSLGAPVQVTGDK
                     KNNRSLALNSKRTLGVYLSGRDEVRLLELSTMESRTLVKDEIWGLQNSTPGFSPNDEY
                     VVYTAYKNFEQDLYAIHLKENKIINLTRTDVTETGPQWSSDGHYVYFISQRLKPAYPF
                     GMGDAKVYRLPLEKLDAPFKMDKYNELFKEEKKDTTKISPADSLKPILIDPDGIMERV
                     EQISPALGTQFLQALYQKGDQTSVLYSSNHGEGKYALWKTVIGPFEPNKTEKISGTDN
                     ASRFDIVEAGNKLYSLHAGNVHKLNLDGNKAELININYTFRKNLSDEFNQMFYEAWAQ
                     MEENFYDGQFHGLDWQKAREQYKAYLPHLNTRADLRVLLNDLLGELNSSHQGFGTFGD
                     DETIDLQNQTMETGIVFEPAHPYTVQRIVKRSAADKKGIDIRPGDVLIKVNEETVDPS
                     RDRHAYFTRPSRDQELSLVFSRQGQAVTARLHPQASLFNEYYEEWIDQNQKRVDDKSN
                     KRIAYGYMKNMGRGELDQFIIDMTGELNQKEALILDLRYNTGGNVHDEVLKFLSQRSY
                     LQWKYRDGKLSPQSNFAPSDKPLVLLTNEQSLSDAEMTAQGFKALKLGKIIGNETYRW
                     IIFTTGIGLVDGSFIRMPSWGCYTMEGKNLEQTGVQPDILIINGFVDKLNGRDPQLDR
                     AIDEILKLLK"
     gene            complement(6593..9901)
                     /locus_tag="PKM27_16355"
     CDS             complement(6593..9901)
                     /locus_tag="PKM27_16355"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNR08892.1"
                     /translation="MIRPGLFLLILITGFSCKTKQDPPVAESIKPVFKKLDSGETGIA
                     FNNVVDENYGNYFDAFAYVYNGGGVAAGDINQDGLPDLYFTGNEVENKLYLNLGNLQF
                     KDITEEAGVGGGPGWDNGVTMVDIDQNGLLDIYVCRGGWQDKDEQRKNLLYINEGNLR
                     FTEKAAEYGLDDAGYSMHAAFFDMDRDNDLDLYLTQRPDSFYLPLSKMISGKLNPPDK
                     NRDKLYKNVSGKFMDVSDAAGLGHGYGYALAVVTADLDRDGWQDLFVSNDYADQDYLF
                     INQRDGTFREAIKESTNHLSLFSMGADIADLNNDGWEDILVMEMLPENYKRSKVSMPR
                     MDVQGFWAIVDGGMHKQYMHNALHLNHGNGFFSEISQLAGIAKTEWSWSTLAGDFDND
                     GLRDIFVANGYRRDLFDGDIKNKLDAFLAANQEKYLSNEDFFTRGFKEFISLYDPIKV
                     VNYLYRNKGDLQFENVSAAWGFADSSFSNGAALADLDRDGDLDLIVNNLEAEAFLYEN
                     TNPGRNHFLKIQLNGPEGNRQGIGTKITLFLGNTIQYFEQKTVRGYLSSNDPVIHFGL
                     GKIQSVDSVRVQWPDGKENRIPATAANTLLQVNYGEAHMPAPAPEPAPVLFSLETKAL
                     STPFKHHETPLDEYKDQVLLPHEFSKSGPFIAAGDVNGDQNQDFYVGGAEGQAGCLYL
                     YSNQRFIKQNTPVFEQDKSYEDLGASFFDADGDGDLDLYVVSGGAGHPEGSGLYQDRL
                     YLNGGRGNYSKKELPKTLSSGSCVLPFDLDGDGDLDIFRGGQVVPNRYPFPALSYLWI
                     NDKGSFTDGSALFPHLSGLGMVNTAVHTDLNGDRSPELVISGEWMPLRIFEFKNGKME
                     EATEALGLGQTHGWWNRIVADDLDEDGDMDLIAGNLGKNYKFIASREKPFEVYATDFD
                     RNGTNDIFLAKYDHGVQVPIRGRECSSQQMPAILQKFPSFLSFAVSDIRGILGPGIDR
                     ALHLKAYEFQSALFINDQGVLRFKPLPVFAQLSTVNGIIVRDVDGDGHKDILIAGNRF
                     DVEVETSPADASPGMYLKGLGKNDFEAIGPLHSGFSVPYNVKDIQWLDSGNRRYVLVG
                     INDGPLLCYRVNPQ"
     gene            10050..11351
                     /locus_tag="PKM27_16360"
     CDS             10050..11351
                     /locus_tag="PKM27_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNR08893.1"
                     /translation="MKRLLTNLTFWVLTAITLGILLGHYYPEWVLKPILEKEWKGHFL
                     GQELKVGRTLAEFLGNGFISIVKLFINPIIFLTITLGIINMGDLKKVGKVGVKALIYF
                     EVVTTAALLIGVVMANIIKPGFGVIPAGQAGEVSRYADQAADFSWWQFFKDNSTIQIL
                     MLALFSGIVLNKFSFRHRVVDALVPVSKWVFRALYMVMLLAPIGAFGGMAFTIGKYGL
                     KALFPLGKLMLTVYCTMALFIFIVLWYILRYYQVNLWKFLKYIKEELLIVLGTSSSEA
                     GLPNLMVKLERMGCSKPVVGLVVPTGYSFNLDGTTIYLSMAILFLAQVYHVHLSAGQI
                     LSIIGILMVTSKGAAGVTGSGFIVLATTMAAVKVIPIEGLALLLGVDRFMSEARAITN
                     FIGNGVATIWIANNENEFDRNKCEVALNKGNPPPPRSSEEP"
     gene            complement(11397..12521)
                     /gene="bshA"
                     /locus_tag="PKM27_16365"
     CDS             complement(11397..12521)
                     /gene="bshA"
                     /locus_tag="PKM27_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNR08894.1"
                     /translation="MRIGIVCYPTFGGSGVVATELGLGLAKRGHEVHFITYKRPVRLS
                     HFMTNIYYHEVNALDYPLFEYTPYDTTLASKLVDVIKFEKIDLLHVHYAIPHATIAFI
                     VKKILETQGIRIPVVTTLHGTDITLVGADPSFAPVVEFSINQSDGVTAVSRDLKDDTL
                     RYFNISRDIRVIYNFIDFDRFRKIDKEHFKKALAPDGEKILIHTSNFRKVKRVDDVIH
                     IFQKVIQQVPCKLLLIGDGPERQRLEEICRKIKVCEHVRFLGKQEAIEELLAVSDLFL
                     IPSENESFGLAALEAMACHVPVISSNAGGLPELNLDGTSGFVHPVGDIEGMAASAIRL
                     LKDETLLDQYRKQAFERAREFDIDKILPQYEDYYMEVMKG"
     gene            12846..>13369
                     /locus_tag="PKM27_16370"
     CDS             12846..>13369
                     /locus_tag="PKM27_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR08895.1"
                     /translation="MKKELPGDLSYHGLHHTEDVLNAALKIAKTEKVAVKDVKLLRLA
                     SLLHDSGFIYTYKGHEEMGCKIAREILPGFHLPRHEIEVICGMIMATKIPQTPDTILE
                     RIICDADLDYLGRDDVFAIADTLFREIKLYINPISAEEWNNLQISFLEAHEYFTKFSK
                     TKRAPAKLIYLNHL"
BASE COUNT         3025 a         3398 c         3475 g         3471 t
ORIGIN      
        1 gtaacctccc tttctcggtg cgtcaccggt tttgggcagt ctgcggatgt tgccccagtt
       61 gtcgtcgcac agcaataagg tgacatcatc cggtacgcgc atgccttggt cgtaataatc
      121 ctggacttct ttatacaatg cccagagttg aggggtttcc ggggcgggtt taccggtgac
      181 ttcttcaatg atctggcgct gatccctgac gattctttcc agcaacgcgg tagccgtctc
      241 acgactcatg ggttcatccc cgtctccgcg catgccaatg ctgacgattt tttcatggtt
      301 tgccctttgc atcccccctt tccagaattc ttttaatcct gcttccgtac tgtcgtaatt
      361 ccacttttgc ctcttgccat accgcctcca ttcgtcgtgc gcgcgcatga ggggttcatg
      421 atgagaggta ccgatgacga tgccgtataa atcggcgtaa gcccggttca taaaatcgtc
      481 atcgtaaaag gcgttgcccc acatggccgg ccagaggtaa ttgcccttaa gccggaggat
      541 gagctcaaat actttttgat aaaacagatg attgaatcct ccgaattttt ccctggtcca
      601 tccggagagg gcgggggcct cgtcgttgat gaagatgccc cggtatttca ctttcggggc
      661 atccgtgacg agtaatttgt ccttgacata caaggcctcc tttttaacca caggtacatc
      721 cgcccaccaa taccaggggg aaacccccat ctgtttgcac aactcaaagg cgccaaaggc
      781 catgccgcgc cggtcgctgc ccgcgagaag caggcttttc tgtccggagc cgctttggtc
      841 caccaccgat accaggtagg tttcccactt cccctgaagg ctttcgaggt ttatatgctg
      901 ttgtgctgcc caggctttga ccagggatga accggcgatg gtgccaagca ggaccggatt
      961 ttttattccg gccaggcgcg ccggatccgc atcggatatg atttcgggat ggatgccgaa
     1021 gaccatttcc agatcctggc gaaacaattc ggcggttttc agggtgaggg cttcatcccc
     1081 ggggtcgatc acaatcgccc ctgcctggtg gagcgggaat ccggattcgg aactcagcca
     1141 ggactgggca aacaaggggt tcaaaaaaat aagcattatg aatgcctgga tggccctgca
     1201 caggcgataa cctctataca tatagaaata agtttaaata atgaatccgt ttacctggat
     1261 tcaatctggt cgagggcttc caatgcagta gagaccggca atttacaaac tttatcccgg
     1321 catacataga tggtggtttc gccttctttg tattttcctt tcgtcagagg cagggattcc
     1381 tcccgtcccc ccatataaac cgtaaaagga agataatgcc gtcgcatttc ctggttaacc
     1441 cgcggggcct gatctccgag gataacgatc tcatggctgc cgcaggcgat ccatccatac
     1501 agactgatcc aatggctgaa actggaagga taggccagga tgcccggaag cacttctttc
     1561 accatggcct ccgcacggga caaatagccg ctgtcctgaa gcagggtacc catcttgtat
     1621 agattgcggg ccatgaccgc gttggaggaa ggcagggctt catcatagtg gtccttccgc
     1681 cgggtaaata aactggtttc ctccgggctg taataaaaca aggcggaatt ttggccggaa
     1741 aaatcgacga tgacccgctt catcagccgg tccgactgct gcagccattg acggtcgaag
     1801 gtagtctcat acaaggccag cagggatttt atggccaggg catagtcatc cagaaaccca
     1861 tagtttgaac cggtgcgcaa aagacggcca tccggtttta gctgagtttg cagcagaaag
     1921 gacgccgttt gaagcgcctg gcccaggaag aggctatcgc ccagggcgtg aaaagcatcc
     1981 aggcaggcac cgatcgcgag ggcattccag gaagtaatga ttttggtatc ggtcgccggg
     2041 gcaatgcgcc tctcgcgggc agccaccagt ttggcccggc aggactccag catctccttt
     2101 tgggatgccg aaaaattttc agctccgtcc aggtaaagga cattatcctt gttttcatcc
     2161 tgtgaaatcc gataggcctt ttcaaacagg gcgaattctt caggacttaa gagatccctc
     2221 agcgctttcc gagtccacag ataatatttt ccttcctcgc cttcactgtc tgcattgacc
     2281 gaggaataat atcccccggc cggcgctttt aattcttttt ccataaaatg caggctttcc
     2341 cggacgattt ttgcataccc tggtttgcca gtgacctgaa aagcctcgct gtataaggaa
     2401 atgagctggg cattgtcgta caacattttt tcaaaatggg gccattgcca aaaacgatcg
     2461 gttgaatatc gggagaatcc tcccccaagc tgatcataga tccctcccga ggccatggcg
     2521 tccaaagtgc ggacgacctg ttccagggcc gtactgtctt ttttccagga ctggtattcg
     2581 agaagggtca gccatacgga gggcatggga aacttgggtt ccccctcaag gccgccatag
     2641 tccggatccg aatggcggat caggctgtcg gcaagggctt ggtatagacc ggtacggaag
     2701 gccgtatcgg ccggggccgg caaccgcacc agggggtcgg cccggatatc ctgttccagc
     2761 aagagggcct gctgcctgat ctttcccggt tgggcccggt acaaggcggt gatctgagac
     2821 aggaggtctg tccactggtt tttttccata taggtgaccg cgtggaaagg cctgccatcc
     2881 ggtaaggcaa agacattggc cggccaacct cccgagccct ggatcagttg cagggcgtgc
     2941 atgtacaact ggtcgatgtc cggcctttct tcccggtcga ttttaatgca gataaaatga
     3001 tcattcatca aacgggccac agccgtatcc ataaaacttt cctcttccat cacatggcac
     3061 cagtgacagg aagcataacc catgctgatg aggatgggcc gatccagttt ttttgccagt
     3121 tcaagggctc cttcgttcca gggatgccag aggacggggt tgtccaggtg ctggagcaga
     3181 taaggactgg tggatccctc cagaggtttt ttatttttgc tgcctttgca atgtgtgaac
     3241 agaaaaaacg gaaagatgaa tagaaagcac aggccttttg ccttagttat cctgatcccc
     3301 caggctccca aatttttgat cacttcaaca gctttagaat ttcatcaatc gcccggtcca
     3361 gctggggatc ccttccgttc aatttatcca caaaaccgtt gatgatcagg atatccggtt
     3421 gtactccggt ttgttccagg ttttttccct ccatggtata acaaccccag ctgggcatgc
     3481 ggatgaatga accgtcaacc aggccgatgc ccgtggtaaa gatgatccag cggtaggttt
     3541 cgttgccgat gattttaccc agtttcaggg ccttaaatcc ctgggcggtc atttcggcat
     3601 cgctcagcga ttgttcattg gtcaaaagga ccaggggttt atcgctgggg gcgaaattgg
     3661 actgtgggga taatttgcca tcccggtatt tccattgtaa atacgaacgc tggctcaaaa
     3721 acttcaatac ttcgtcatgg acatttcccc cggtattgta acggaggtcc aggatcaggg
     3781 cctctttttg attcaattcc ccggtcatgt cgatgataaa ctgatcgagt tctccccggc
     3841 ccatgttttt catatagccg tatgcgatcc gcttattgct tttatcgtcc accctttttt
     3901 gattctgatc aatccattcc tcataatatt cattgaataa agaagcctgc ggatgcagcc
     3961 tggccgtgac ggcctgtccc tggcgggaga aaacgaggct taattcctgg tcgcgggaag
     4021 gccgggtaaa ataggcatgg cggtcccggg aaggatcaac ggtctcctca ttcactttga
     4081 tcaacacatc cccgggccgg atatcgatgc cttttttatc cgccgcggac cgctttacga
     4141 tcctttggac ggtataggga tgggcgggtt caaacacaat gccggtctcc atggtttgat
     4201 tttgcaggtc gatggtttca tcgtccccaa aggtcccgaa gccctggtgg gaagaattta
     4261 attcacccag cagatcattc agcagcaccc gcaggtccgc acgggtattg aggtggggga
     4321 ggtaggcttt atactgctct ctggcttttt gccaatcgag gccgtgaaac tgcccgtcgt
     4381 agaagttttc ttccatctgg gcccaggctt cataaaacat ctggttgaat tcatcggaaa
     4441 ggtttttacg gaaggtataa ttgatattga tgagttccgc cttattgccg tccaggttca
     4501 atttgtggac attgcctgcg tgaagcgaat agagtttgtt cccggcttcc acgatgtcaa
     4561 accgggaagc attgtccgtg ccgcttattt tctccgtttt gttcggttcg aagggcccta
     4621 tgacggtttt ccaaagggcg tatttccctt cgccgtgatt gctggaatag aggactgaag
     4681 tctggtctcc cttttggtat aaagcctgta gaaactgggt ccccagggca gggctgatct
     4741 gttcgacgcg ttccatgatc ccgtccggat cgatcaggat gggtttgagg ctgtcggccg
     4801 gtgatatttt ggtcgtatct tttttttctt ccttaaacaa ctcgttgtac ttgtccattt
     4861 taaacggggc atccagcttt tccaggggca gccggtaaac cttggcgtcc cccatgccga
     4921 atggataggc cggcttcaaa cgctgggata taaaatagac gtaatgacca tcagacgacc
     4981 attggggccc ggtctccgtg acatcggtcc gggtcaggtt gatgatttta ttttccttga
     5041 gatgaatggc ataaaggtcc tgttcgaaat tcttataggc ggtgtaaacc acatattcat
     5101 cgttaggcga aaatcccggc gtgctgttct ggagtcccca gatctcatct ttgaccaggg
     5161 tcctgctttc catcgtggag agctccagaa gccggacttc gtcgcggccg ctgaggtaca
     5221 cgcccagggt ccgttttgaa tttaatgcca ggctgcggtt gtttttttta tccccggtga
     5281 cctggaccgg ggcgcccagg ctgtccgccg gcatggtaaa ccagtttgaa tatccgtcca
     5341 gcgtctggtt aaaaagcagg gtcttattgt tggagagcca ttttacttct ttcacccttt
     5401 cggaggtagg gcgttttaaa tgcctgataa atttgccttc cgcatcgctt acaaacagtt
     5461 ctccccggga taccagggcc attttctttc catcgggcga gaggtcgtag gcggaaatct
     5521 ggcctttgag ttcgaagtcc ttttctttcg ggaggatggg gttgcggtgg attgaaatgg
     5581 ccaggggccg tgctttgccg ctgcggactt catagatcca gagctgatag tccttttcaa
     5641 acaccacgcg gccgccctgg gcgttgacct ggggggattt gatggaggtg gaaaaggagg
     5701 tcagggcttt tggagtgttc cggttcaggg tgtacaaatt gtattctccg tttgcctggt
     5761 cggagatata atagatattt cctttccggt cgatggtagt cccaaagtct ttgccttccc
     5821 aggttgtata ttttttatac tgttttgttt tgggatgata ggattggata tccgggttga
     5881 atgggccttt gtaacgtttc cgggcaagct ggttgtcgct ctcccaagta tcgttgaaaa
     5941 acaattcccc ggtgaccggg tgttcggcca ggttatggtc atagagaaaa aattgatctc
     6001 caaacaccct gaccggcgta cctccttctg cgctgacctg gtgagaggcc accttccccg
     6061 tgatataact gttgaaatag atcgtttttg aatcccaact ccagcttcgc atctcctcat
     6121 tcgcgctgtg atgggtgagg gtccttacct ccccgccctt cagcggaatc acgaagacat
     6181 cgccgttgcc gtattgcctg ccggtaaagg caatccactg gccgtcggga gataccctgg
     6241 gggaggactc atatccctgc atcgcggtaa gccgggtggc cattccgtcc tggacgtccg
     6301 ctttccacag gtccccttca aaacagaaga ccaccgtttc cccgtccggg gtcaggcttg
     6361 gattggacag gaaataggca gggttttgag acctcaatcc gaaagggatg aaagccagga
     6421 ggatatagaa catttggggc atcagaaaaa atttggaata gaaaattaat acaaatccgc
     6481 ttgtgagtca gtgtaaaacg aatcccaaaa ccgaaccggt tttggggagc gcagggcagg
     6541 tcctattgtc caatttttga attcccgctt tgtacatgga aagcggatca gcctattgtg
     6601 gattgacccg gtaacaaagc aggggcccgt cattgatgcc caccaggaca tagcgtcggt
     6661 ttcccgagtc cagccattgg atgtccttta cattgtacgg cacggaaaag ccgctgtgca
     6721 gtggaccaat cgcctcgaag tcatttttgc ccaacccttt gagatacatg ccgggggagg
     6781 catccgcagg agaggtctct acctccacgt caaaacggtt tccggctatc aggatgtctt
     6841 tgtgaccgtc cccatccaca tccctgacaa taattccgtt gaccgtggat aactgtgcaa
     6901 aaacgggcag gggcttgaat cgcagaactc cctggtcgtt gataaaaagg gccgactgga
     6961 attcataggc tttgagatgg agggcccggt caatcccggg tcccaggatc ccgcgaatat
     7021 cgcttaccgc aaaggacagg aaggatggaa atttctgaag gatggcgggc atttgctggc
     7081 tgctgcattc cctgccccgg atgggtacct gtaccccgtg atcgtatttt gccagaaaaa
     7141 tgtcattcgt cccgttgcgg tcgaaatcgg tggcgtaaac ctcgaagggt ttttccctgc
     7201 ttgcaatgaa tttataattt ttgcccaggt tgcctgcgat caggtccata tccccatcct
     7261 cgtccaggtc gtccgcgacg atccggttcc accagccatg ggtttgaccc agtcctaaag
     7321 cttcggtggc ctcttccatt ttcccgtttt tgaattcgaa gatgcggagg ggcatccatt
     7381 cgcctgaaat gaccagttca ggggaccggt ccccgttcag atcggtatgg acggcggtgt
     7441 tgaccatgcc gaggccggac aaatggggga acagggcgct gccatccgta aagcttcctt
     7501 tgtcattgat ccacaaatag gagagcgcag gaaaagggta acggttgggg accacctgcc
     7561 cgcccctgaa aatatcgaga tccccgtcgc catccaggtc aaaaggcaat acacaggaac
     7621 cgctgctcag ggttttgggc aattcctttt tggaataatt tccgcggccc ccgttgagat
     7681 acaaccggtc ctgatacaaa ccggaccctt cgggatggcc ggccccgccg gaaacgacat
     7741 agaggtccag gtccccgtcg ccatcggcat cgaaaaagga agcgcccagg tcttcatatg
     7801 atttatcctg ttcgaatacc ggtgtgtttt gtttgataaa cctttgattt gaatacaggt
     7861 aaaggcagcc ggcctggcct tcggcgccgc ccacataaaa atcctggttt tgatccccgt
     7921 tgacgtcacc ggccgcgata aacggtccgc tctttgaaaa ttcatggggc agcaagacct
     7981 ggtccttgta ttcgtccagc ggggtttcat ggtgtttgaa tggggttgaa agggctttgg
     8041 tctccaatga aaacaggacg ggggcgggtt ccggagccgg cgccggcata tgggcttccc
     8101 cgtaattcac ctgcagcagg gtatttgcgg cggttgcagg gatccggttt tctttgccat
     8161 ccggccattg cacccggaca ctgtccaccg attggatttt acccaggcca aagtggatca
     8221 ccggatcatt ggaggacagg tatccgcgta cggtcttttg ttcaaaatac tgaatggtat
     8281 tccccaggaa cagggtgatt ttggttccaa tgccctggcg atttccttcc ggcccgttca
     8341 attggatttt aagaaaatga ttgcggccgg gattggtgtt ttcatataaa aaagcttccg
     8401 cttccaggtt gttgacgatg aggtccaggt ctccatcccg gtcgagatcg gctagggccg
     8461 ccccgtttga aaaactgctg tccgcaaatc cccaggccgc acttacattt tcaaattgca
     8521 gatccccttt attccggtaa aggtagttga ccaccttgat gggatcgtag agactgatga
     8581 attccttgaa tccccgggtg aaaaaatcct cattggacag gtatttttcc tggttggcag
     8641 caaggaaggc atcgagtttg tttttgatat cgccgtcaaa caagtccctc cggtatccgt
     8701 tggccacaaa aatgtcccgc aggccatcat tgtcaaaatc gccggccagg gtggaccagc
     8761 tccattctgt tttggcaatc cccgccagtt gggaaatctc actgaaaaac ccgttgccgt
     8821 ggttgaggtg gagcgcattg tgcatgtact gcttgtgcat gcccccatcc acgatggccc
     8881 aaaaaccctg aacatccatg cggggcatgg atacttttga gcgtttataa ttttccggca
     8941 acatttccat gaccaggatg tcttcccaac cgtcgttgtt gaggtcggcg atgtcggctc
     9001 ccatcgagaa cagggagagg tgattggtgc tttctttgat ggcctcccgg aaagtgccgt
     9061 cccgctggtt gataaaaagg tagtcctggt ccgcataatc gttggaaaca aagaggtcct
     9121 gccagccatc ccggtcgagg tccgcagtga ccacggccaa agcataacca tacccatggc
     9181 ccaggcccgc ggcatcgctg acatccataa atttgccgct aacgtttttg tagagtttat
     9241 cccggttttt atccggcgga ttgagttttc cggaaatcat ttttgacagg ggcaggtaaa
     9301 aggagtccgg ccgctgggtc aggtagaggt ccaggtcgtt gtcccggtcc atgtcaaaaa
     9361 atgcggcgtg catcgaataa ccggcatcgt cgagcccgta ttcggcggcc ttttcagtaa
     9421 aacggagatt gccttcgttg atatacaaca ggtttttcct ttgttcatcc ttatcctgcc
     9481 atcctccccg gcaaacatag atatccagga ggccgttctg gtcaatgtct accatggtga
     9541 ccccattgtc ccagccgggc ccgccgccca cccccgcttc ttccgtgatg tccttgaatt
     9601 gcagattgcc gaggttgagg taaagtttat tctccacttc gttgccggtg aaataaaggt
     9661 ccgggaggcc gtcctggttg atatcgcccg ccgccacacc ccctccgttg tacacatagg
     9721 caaaggcatc aaaataattt ccatagtttt catcgaccac attgttgaag gcaatccccg
     9781 tttctccgga gtccagtttt ttaaacacgg gctttatgga ttctgcaacg ggcggatcct
     9841 gtttggtctt acaggaaaat cccgtgataa ggattaataa aaataaacca ggtcggatca
     9901 tgtgtcaaat ctttaaaaag ttagcaagtt agcaagttgg taagttagca agttggtaag
     9961 ttagtaagtg ggtgggcggt tcaatgcaac cctggcagat aggcccttaa ctcttctgga
    10021 cctcattcgt ttcattatat ttaccgcgaa tgaagcgcct gctcaccaac ctcacttttt
    10081 gggtactcac tgccattacc ctgggtattc tcctgggcca ctattatcca gagtgggtgc
    10141 tcaagcccat tctcgaaaag gaatggaagg gtcatttcct gggccaggag ctcaaagtgg
    10201 gcaggaccct ggccgaattt ctcggtaacg gttttatatc catcgtcaaa ctgttcatca
    10261 atccgatcat tttcctgacc atcaccctgg ggatcatcaa tatgggcgac ctgaaaaaag
    10321 tggggaaggt gggggtcaag gcgctgatct attttgaagt cgtgaccact gcggccttgt
    10381 tgatcggggt ggtcatggcg aatatcatca agcccgggtt tggggtgatc cctgccggtc
    10441 aggccggaga ggtgtcccgg tatgcggacc aggcggcgga cttcagctgg tggcagtttt
    10501 tcaaggataa ttcgaccatc cagattttaa tgctggctct gttcagcggg attgtgctca
    10561 ataaattcag tttcaggcac cgggtggtcg atgctctggt cccggtatcc aagtgggtat
    10621 tcagggccct ttatatggtg atgctgcttg cgcccatcgg ggcatttggc ggcatggctt
    10681 tcacgatcgg gaaatacggc ctcaaagccc tgttcccgct gggcaaactg atgcttaccg
    10741 tctattgcac catggccctg tttattttta tcgttctctg gtatatcctc cggtactacc
    10801 aagtgaacct ctggaaattc cttaaataca tcaaagaaga attactgatc gtcctgggta
    10861 cctcctcttc cgaagcgggc ctgcccaacc tgatggtgaa gctggagcga atgggttgtt
    10921 caaaacccgt ggtgggtctg gtagtgccca caggatattc ctttaacctc gacgggacca
    10981 ccatttatct atccatggca atcctctttc tggcccaggt gtatcatgtc catctcagtg
    11041 caggccagat tttaagcatt atcggcatcc tgatggtcac ctccaaagga gcggccgggg
    11101 tgacgggcag cgggtttatc gttttggcta ccacgatggc cgcggtaaaa gtcatcccca
    11161 tcgaaggcct ggcattgttg ctgggagtgg accgttttat gtcagaagcc cgggccatta
    11221 ccaattttat cggaaatggc gtggccacca tttggattgc caacaacgaa aatgaattcg
    11281 accggaataa atgcgaagtt gctttgaata agggaaaccc cccaccgccc cgaagttccg
    11341 aagagcctta aatcgacacc acccaccaac caccaaccac tacacaccac ccacccttat
    11401 cctttcatca cctccatata ataatcctcg tactgcggaa ggattttgtc aatatcgaat
    11461 tcccgggccc gctcaaaagc ctgcttccgg tattgatcga ggagcgtttc atccttgagc
    11521 agccggatgg cactcgcggc catgccttcg atatccccga cggggtgaac aaatccgctt
    11581 gtcccgtcca ggttcagttc gggcaatccg cccgcattgg aagagatgac cgggacatga
    11641 caggccatgg cttccaaagc cgccagtccg aaactttcat tttcgctggg gatcagaaac
    11701 aggtcgctga cggcaagcaa ctcctctatg gcttcttgtt ttcccaggaa ccgtacgtgt
    11761 tcacagacct tgattttccg gcagatttcc tccagccgct ggcgctccgg gccgtcgccg
    11821 ataagcaaaa gtttgcaggg tacctgctgg atgactttct ggaaaatatg gatcacgtcg
    11881 tccacccttt tcaccttcct gaaattggag gtgtgaatca gtattttttc cccatcgggg
    11941 gcaagggctt ttttaaaatg ctccttgtcg atcttccgga agcggtcaaa atcgatgaaa
    12001 ttgtaaatca cacggatgtc ccggctgata ttaaaataac ggagcgtgtc gtctttcaga
    12061 tcacgggaaa cggccgtgac gccgtcggac tggttgatgc tgaattcgac caccggtgca
    12121 aaggaaggat cggccccgac gagggtgatg tccgtcccat ggagggtggt gaccacggga
    12181 atgcggatgc cctgggtttc cagtattttt tttacgataa aggcgatggt ggcgtgcggg
    12241 atggcatagt gcacatgcag caagtcgatt ttttcgaatt tgatcacatc gaccagtttg
    12301 ctcgccaggg tggtgtcgta gggggtgtac tcgaaaaggg ggtaatccag ggcattcacc
    12361 tcgtggtaat agatattggt catgaaatgg cttaggcgga ccggtctctt gtaggtgatg
    12421 aaatgcactt cgtggccgcg tttggccagg ccgaggccca attccgtggc cactaccccg
    12481 gaacccccga aggtggggta gcagacaatt cctatacgca tccctgcaag ttagaaattc
    12541 tgtgattaag ttgaagccca ggggtgtttt aaaggataaa gcggctaggt ttaataaata
    12601 gtttaatttt ttttgttatt tgtaactgat aggttatcaa tttaatataa cccaaaaccg
    12661 gcttttttaa tccggaattt ttcatttcct ttattttaac tgaaatttgg actcattttt
    12721 gtaatcattc agcaataacc gaaccgggat ttgcagggaa aatctatatt tatgaaattg
    12781 ccttgttttt tatgagacaa aaagctcctg aatataaaga agctgaagca gcgattcggt
    12841 taaaattgaa aaaagaactt cccggtgatc tttcttatca cggattgcac cacaccgagg
    12901 atgtgttgaa tgccgctctt aaaattgcca aaaccgaaaa ggttgccgtg aaggatgtca
    12961 aattgctgcg cctggccagt ttgcttcacg attcaggatt tatttatact tataaggggc
    13021 acgaagaaat gggttgcaag atcgcccggg aaatcctgcc cggctttcac ttgccgcggc
    13081 atgaaatcga agtgatttgc ggtatgatca tggccaccaa aatccctcaa acccccgata
    13141 ccattttgga acggattatt tgtgatgcgg acctggatta cctgggccgg gacgatgttt
    13201 ttgccattgc cgacacccta ttccgcgaaa taaaattata cataaacccg atttctgccg
    13261 aagaatggaa caatcttcag atcagtttcc tggaggcgca tgaatatttt accaaatttt
    13321 caaaaaccaa aagggctccg gcaaaattga tatacctcaa tcatttaaa
//
LOCUS       DAOQXT010000118        13305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5157_length_13305_cov_6.455245, whole genome shotgun sequence.
ACCESSION   DAOQXT010000118 DAOQXT010000000
VERSION     DAOQXT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5157_length_13305_cov_6.455245"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            721..4095
                     /locus_tag="PKM27_16375"
     CDS             721..4095
                     /locus_tag="PKM27_16375"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNR08896.1"
                     /translation="MRPFFYTLLLLILWIPLQQSCSPAGKGHVLVFSLTKGYRHASIE
                     KGQEAIKKLGAENGFEVTISEDPGLFTDESLQHFSAIIFLSTTGDILNSNQEAAFMRY
                     IQAGGGFVGIHAAADTEYGWPWYGKLVGAYFHSHPKQQNATISVFCDGHASCTHLPTE
                     WKRWDEWYNYRDVSPDIKVIASLNESSYEGGNMNGKHPISWYHEYDGGRSFYTGLGHT
                     DSSFVEEPFLKHLLGGIRYAMSKKKPDYSKSYTLDAPEESRLEKTVLASNLDEPMQMA
                     PMKDGSVLIAERKGNLKLYNPNTGLIEYVASMPVHSKFEDGLLGMALDPDYENNHYLY
                     LFYSPVGEKSVQHVSRFVFNNKELDLSSEKLILEIPVQREQCCHSAGYIKFGRDGLFY
                     ISVGDNTNPFASDGFNPIDERPGRIPWDAQKSSANTNDLRGKILRIKINPDASYDIPE
                     GNLFKPGTPKTRPEIFVMGCRNPFRFSIDARRNLVHWGDVGPDAGKDSVGRGPKGYDF
                     FGQAGTGGGFFGWPYIRGNGHQYHDYDFATGKSGEKFDPAHPVNNSPNNTGLNELPPY
                     KAPKVWYSYDQSPEFPFVGTGGKNPMAGPVYYSDDFKDAPNAFPSYYDGKLFIYEWMR
                     RWIYVVTLDKDHNFIKFDPFMPSTVFANPMDMEFGPDGSLYVLNYGTGWFSRNADAQL
                     FKINFNGGNRQPLAKMNLDKMTGAAPLTITASASESMDADQDNLTYQWSINGRPRATG
                     SQAKLVIETPGSYDITLVVTDEAGAKSTTHEKVLVGNEMPVVNIEVFGNKSFYWPGRK
                     IPYAITIRDKEDGSSETGTLSPQNIQIASTYIKEGQDMAQLAAEGHLAGMNRSPFETG
                     RLLIEKSDCKTCHTVDKAINGPSYTQISQRYQGKAGIEPVLVQKVITGGLGNWGDRAM
                     AAHPQLSEAEVKQMVSYILSLAAPQGGESAAVSNMLSGVIDVKESGGGTYVISAGYTD
                     RGYNGMAPLSKQTTYNLRSPVLAAHMPDFKSKGVSAYEGNGMKVSIVNSGGYIGFDDI
                     DFEDLKGMDLTYLASQAGRAMIYLDDPENGKLIGEAELVPSDIQAAPKVMTANLNLKF
                     TKDVHKLYIVYAPADSTAQGGMFIPSRIELKFGEGL"
     gene            complement(4259..5602)
                     /gene="argH"
                     /locus_tag="PKM27_16380"
     CDS             complement(4259..5602)
                     /gene="argH"
                     /locus_tag="PKM27_16380"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNR08897.1"
                     /translation="MKLWDKNKSTDQLVEVFTVGNDRELDLVLAPYDALASIAHVDML
                     AATGLLSKDEGLQIRKELQQIYHQALQGKFVIEPGAEDVHSQIEMELTRRLGETGKKV
                     HSGRSRNDQVLVALKLYFRAEIEQMVTGTQTLFDLLQELSTKYQEVLMPGYTHMQVAM
                     VSSFGLWFGAYAEALADDLRSMQAAFQLANQNPLGSAAGYGSSFPLDRTMTTRALGFQ
                     DMHYNVINAQMSRGKTELALGFAMATLAATINKLAMDVCLYAGQDFGFISFPDDLTTG
                     SSIMPHKKNPDVWELIRSHCNRIAALPNEVQLMIHNLPAGYHRDFQLLKEVLFPRIQA
                     MKSVLDLSSFMLRHITVQTGLILQEKYIYLFSVEEVNRLVLQGVPFRDAYKQVGLDIE
                     KGQYKPDYNIHHTHEGSIGNPCTSEIRAKMEGVLYGFDFETVKESLDKLLLPLSE"
     gene            complement(5691..6764)
                     /locus_tag="PKM27_16385"
     CDS             complement(5691..6764)
                     /locus_tag="PKM27_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNR08898.1"
                     /translation="MPIDDTELHQLTDQAIALLQALIPIPAYSREEHAKADKLEEELS
                     KLGLQPQRVLNNLILGRRDDSSRPVLLLNSHIDTVRPVETWTFNPHEPVLREDKIFGL
                     GTNDAGASVVCLAMAYSHLCKKQQAYQLIFAASAEEEISGQNGILRVLEETGPVQLAI
                     VGEPTGMQMAIAEKGLMVIDAVSKGQSGHAARDEGINAIYEALPDIEWIRNYRFEKVS
                     DHLGASKATVTVIHAGKQHNVVPDSCHYVIDVRSNELYTNEEMLHILQSGVKSVLTPR
                     SLRLKSSGIPLSHPVVQRGLSLGLSTYGSPTLSDQALMPNLSLKIGPGQSARSHTADE
                     FILIEEIKEGIRIYIELLDGLEI"
     gene            complement(6731..7540)
                     /gene="argB"
                     /locus_tag="PKM27_16390"
     CDS             complement(6731..7540)
                     /gene="argB"
                     /locus_tag="PKM27_16390"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNR08899.1"
                     /translation="MDASDPVLIIKIGGNVLDHPPDTHSFLNKFIGLPHKKILIHGGG
                     KLATDLSARLGMETKMVDGRRITDFETLKIATMVYAGWTNKSLVADLQAMGVNALGLS
                     GADLNLVQAQKRPVRDIDYGWVGDVSKIFIKRWSFLLENGILPVLCAITHDRQGHLLN
                     TNADTLASELACALVEKYPVKLIYCFEKRGVLLDQLDDQSVIPQLDKEAYLRLKEAGI
                     ISKGMIPKLENAFKASAKGIQDVYICAAQNIDQYQTSGTQICPSMIPNSTN"
     gene            complement(7637..10333)
                     /locus_tag="PKM27_16395"
     CDS             complement(7637..10333)
                     /locus_tag="PKM27_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNR08900.1"
                     /translation="MKSLFVFVFLFISVFASAQLQVHDLRVEQLVNPVGLEVAKPHFS
                     WKITSPAKNTMQTAYEITVASAPDFNKKSILWTSKQDSDQSTYIPYGGPMLSSNTRYY
                     WKLAVWDNHSNMAAGPGGQYWHTGLLNPAEWTASWIAAGPTADTAGPSPMLRRVFEIK
                     KDIISATLFISARGLYEAQINGQRVGQDYLTPGWTAYQKRIQYQAYDVTSMLQKGMNA
                     IGVTLGNGWFTGEIGFTKNNKFYGEDLALISQLYVTLKNGMVIRIDSDQAWQSSTGPI
                     RYSEIYHGETYDARKEQAGWSGPDFAATGWVPVVLKDHPKNLVGTYNEPVRKREKIKA
                     VKLIVTPKGEQVLDFGQNMTGWVEVKASGPAGTQIELIHAEVLDKFGNFYIENLRRAK
                     QSNKFVLKGAGMEVFEPHFTFQGFRYVKVMGYPGTINPENFTGIVLYSDMERTGHFEC
                     SNPLVNQLQHNIQWGQRGNFVDVPTDCPQRDERLGWTGDAQAFARTAAFNFGVQNFFA
                     KWLQDLAADQRPNGAVPFVVPNVLSPTAVASAGWADAATIIPWDMYQVYGDKGLLARQ
                     YESMKNWVKYMQDSSTQYLWNRGFHFGDWLFYSLGNDTDGRSAVTDKYLIAQCFFAHS
                     TQLVIRAAEVLGKNEDIAYYKDLLDKIKEAYRKEYVTANGRLVSSTQTAYVLALHFDM
                     LPETMRADAAKRLADNIRAYNHHITTGFLGTPYICHVLSRFGYTDLAYTLLLQETYPS
                     WLYPVKMGATTIWERWDGIKPDSTFQSAGMNSFNHYAYGAIGDWMYRVVCGIEVEEPG
                     YKKSILQPRLTDSLQHAAASTMTPYGPIYSGWKKQNGAVIFEVEIPANTTSTLIIPAM
                     GTSNIMVDDQPLPAQKEIQVLASGQGMVKLGLGSGKYRIKVG"
     assembly_gap    10775..10874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11316..12530
                     /locus_tag="PKM27_16400"
     CDS             11316..12530
                     /locus_tag="PKM27_16400"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNR08901.1"
                     /translation="MKRVYLDNNATTPCDPRVVEAMLPYFTEKFGNAASRSHPYGWEA
                     EEAVDYAREQIATLIHADPKEIIFTSGATESDNLALKGVFDMYRKKGNHIITATTEHK
                     AVLDTCKHLAEQGAEITYLKVNDKGLIDLAELEAAIKPTTILVSIMWANNETGVIQPV
                     KEIGEICAKKGILFMSDATQAVGKIDVNPREMGIHLMAFTAHKMYGPKGVGALYVSRK
                     NPRVKVTAQMDGGGHERGMRSGTLNVTGIVGFGKAAELARKEMDQDMARISKLRDRLE
                     TTLKNKLEEVYINGDPAHRMPHVTNMSFKHVEGEGLMMTFNQRMALSSGSACTSASLE
                     PSYVLVALGLGDDLAHSSLRFSLGRFTTDEDIDIAIQSIEEGVNHMRSLSPIWEMYKE
                     GVDLSQVTWSPH"
     gene            12577..12996
                     /gene="iscU"
                     /locus_tag="PKM27_16405"
     CDS             12577..12996
                     /gene="iscU"
                     /locus_tag="PKM27_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNR08902.1"
                     /translation="MAYSEKVLEHFQHPRNVGTLDKSKNNVGTGLVGAPECGDVMRLQ
                     IEVDEETGTIKDAKFKTFGCGSAIASSSLATEWLKGKTIDDALKIDNMDIVEELALPP
                     VKIHCSVLAEDAIKAAIKDYQTKNGIVAEQTAEAAKA"
     gene            13010..>13305
                     /locus_tag="PKM27_16410"
     CDS             13010..>13305
                     /locus_tag="PKM27_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNR08903.1"
                     /translation="MIFVSDTAKKRIQEIMAKEHLGADHFVRVSVTSGGCSGLSYKMD
                     FDDESRPNDQVFEDNGVKVVTDLKSFLYLFNTTLDFSGGLSGKGFYFENPNASRT"
BASE COUNT         3225 a         3543 c         3276 g         3161 t
ORIGIN      
        1 ggtaatatgt tttaaaatac catattacaa gtctaaagga agcttagctt cggccccaca
       61 tttagatttc gcccagaaaa catataatga tattgtctcg taaaattggg gatcccaatg
      121 gtgcctttag gcacgtcata tgcgtagctc atacgggcag gttatttcgt cccatccggg
      181 acgatatcaa atcccaaaat tatatgctac ccgtatgaaa tccctacggg atcaaatggc
      241 ccatgtttgg acgaagctta gcttccgtcc atccactcat ttaaaaattt tcctccgcga
      301 tctccctccc tccgtgtgaa ccatgttagg acgaagctca gcttctgccc atcacgccat
      361 ttaaaaattt tcctccgtga tctccgtttg gacgaagctt agcttcggcc tcacatttgg
      421 atttcgccca gaaaacatat aatgatattg tctcgtataa ttggggatcc caatggtgcc
      481 tttaggcacg tcatatgggt agctcatacg ggcaggttat ttcgtcccat ccgggacgat
      541 atcaaatccc aaaattatat gctacccgta tgaaatccct acgggatcaa atggcccatg
      601 ttaggacgaa gcttagcttc cgcccatcca ccactttaaa aattttcctc cgcgatctcc
      661 ctacctccgt gcgctcttga ccgataaaac cttattttta caacctttta aataaaaaat
      721 atgcgtccgt ttttttatac cctcctcctc ctgatcctgt ggattcccct acaacagtcc
      781 tgttcgcccg ctggcaaagg ccatgtcctg gttttttccc tgaccaaagg ttaccgtcat
      841 gcctccatag aaaaagggca ggaagcgatt aaaaaactgg gtgctgaaaa cggctttgaa
      901 gtcacgatca gcgaagaccc gggcttgttt acggatgaaa gccttcagca tttttcggcg
      961 attatattcc tcagtacgac gggcgatatc ctgaacagca accaggaggc cgccttcatg
     1021 cgctatatcc aggccggcgg cggttttgtg ggcatccacg cggcggccga cacggaatac
     1081 ggctggccct ggtacggcaa actggtcggg gcctatttcc acagccaccc caaacaacaa
     1141 aacgccacca tcagcgtatt ctgtgacgga catgcatcct gcacccatct ccctaccgaa
     1201 tggaaacgct gggatgaatg gtacaattac cgcgatgtgt ccccggacat taaagtcatc
     1261 gcttccttga acgagagcag ttacgaaggc ggcaatatga acggcaaaca tcccatctcc
     1321 tggtaccatg aatacgacgg ggggcgctcc ttttacaccg gcctgggcca taccgattcc
     1381 tccttcgtgg aagaaccttt cctcaaacac ctgttgggag gcatccggta cgccatgtca
     1441 aagaaaaagc cggattacag caagtcctat actttggacg ctccggaaga aagccgcctg
     1501 gaaaaaacgg ttctcgcgtc caacctggac gaacccatgc agatggcccc gatgaaggat
     1561 ggcagcgttt tgatcgccga acgaaagggc aacctgaaat tatacaatcc gaataccggc
     1621 ctgatcgagt atgtggccag tatgccggtt cacagcaaat ttgaagatgg acttctgggc
     1681 atggccctgg accccgatta tgaaaacaac cactacctct acctcttcta ttctccggta
     1741 ggtgaaaaat ccgttcagca cgtttcccgc tttgttttca acaataagga actcgacctt
     1801 tccagcgaaa aactgatcct ggagattccg gtccaacgcg aacaatgttg ccacagtgca
     1861 gggtatatca aattcggaag ggacggattg ttctacatct ccgtaggcga caacaccaat
     1921 ccatttgcat cggatggctt caaccccatc gatgaacggc cgggccgcat accctgggat
     1981 gcccagaagt cttctgccaa caccaatgac ctgaggggta aaatcctccg gatcaaaatc
     2041 aatccggatg ccagttatga tattcccgaa ggcaatcttt ttaaaccggg aacgcccaaa
     2101 accaggcctg aaatcttcgt gatgggttgc cgcaatccgt tccgtttttc catcgatgcc
     2161 agaaggaacc tggtccattg gggcgacgta gggccagacg cggggaaaga cagcgttggc
     2221 cgcggcccaa aaggttatga ctttttcggc caggccggca cgggcggggg ttttttcgga
     2281 tggccttaca tccggggcaa tggccaccag taccacgact atgattttgc aaccggtaaa
     2341 agcggagaaa aatttgaccc ggcccatccc gtcaataatt cacccaacaa caccggcttg
     2401 aacgaacttc ctccctataa agcccccaag gtctggtact cctacgacca gtcccctgaa
     2461 tttcccttcg tcggtaccgg gggtaaaaac ccgatggccg gcccggtata ttacagcgat
     2521 gattttaaag acgcgcccaa tgccttcccc tcttattatg acggcaagtt gtttatctac
     2581 gaatggatgc ggcgctggat ttatgtggta accctggaca aggaccacaa cttcatcaaa
     2641 tttgatcctt tcatgccctc cacggtattt gccaacccga tggatatgga atttggcccg
     2701 gacggttctt tatatgtact caattacggc accggatggt tttcacgcaa cgcggatgcc
     2761 caattgttta aaatcaattt taacggaggc aaccgtcagc cgctggctaa aatgaacctg
     2821 gacaagatga ccggtgcagc cccgctcacc atcaccgcca gcgcatcgga atccatggac
     2881 gccgaccagg acaacctgac ctatcaatgg tcaatcaacg gcaggccgcg ggctaccggg
     2941 tctcaggcca agctggtgat tgaaacgccc gggtcctatg acatcaccct ggtggtcacg
     3001 gatgaagcag gcgccaaatc cactacccat gaaaaagtgc tcgtgggcaa tgaaatgccc
     3061 gtagtgaaca ttgaagtatt tggcaacaaa tccttttact ggcccgggag aaaaattccg
     3121 tatgccatca ccatcaggga caaggaggac ggatccagcg agaccggcac actcagtccg
     3181 caaaatatcc agatcgcctc gacttatatc aaggaaggac aggatatggc acagctcgcc
     3241 gccgaaggcc acctggcggg aatgaaccgg tcccccttcg aaaccggccg cctcctgata
     3301 gaaaaatcag actgcaaaac ctgccatacc gtggacaagg ccatcaacgg cccctcctat
     3361 acccagattt cacagcgtta ccagggaaaa gccggtattg agccagtact ggttcaaaaa
     3421 gtaattacag gcggtctggg caattggggc gaccgcgcaa tggctgctca cccgcaactg
     3481 agcgaagcgg aagtgaaaca gatggtcagt tatatcctct cccttgcggc tccgcagggc
     3541 ggagaaagcg ctgcggtgtc caatatgctg agcggggtca tcgatgtcaa agagagcgga
     3601 ggcggtacct atgttatctc cgccggatac accgaccggg ggtacaatgg aatggcccct
     3661 ttgtccaaac aaacgaccta taatctccgg agccctgttc tggcagccca tatgccggat
     3721 ttcaagtcaa aaggcgtctc cgcatacgaa ggcaatggca tgaaggtttc catcgtaaac
     3781 agcgggggct atatcggctt tgacgatatt gattttgagg acctcaaggg catggacctg
     3841 acctacctgg ccagccaggc ggggagggcc atgatttacc tggacgatcc ggagaatgga
     3901 aaactcatag gcgaggcgga actggtgccc agcgatatcc aggccgcccc gaaagtcatg
     3961 acggccaatc tcaacctgaa gtttaccaaa gacgttcaca aactttatat cgtgtatgcc
     4021 ccggctgatt ccacggcgca gggaggcatg ttcattcctt cgcggattga gttgaagttc
     4081 ggcgaaggat tataggagtt tctttaggcg tcctcgccta aagttgtaca cattgattac
     4141 ttttttttgt acaagcctca ggcaggacac cctgaaacaa ccacaggaat tattgagtca
     4201 tgtatttcta aatcaattca acagttccgc cattccgcaa ttccacattg cattgagcct
     4261 attcagagag gggcagcaat aacttatcca ggctttcttt taccgtttca aaatcaaatc
     4321 catagagcac cccttccatc ttggccctga tttcgctggt gcagggattt ccaatacttc
     4381 cttcgtgggt atgatggatg ttataatccg gtttgtattg gcctttttca atgtcgaggc
     4441 ccacttgttt gtaagcatcc cggaagggta ctccctgcaa gaccagacgg ttgacttcct
     4501 ccacgctaaa caggtagatg tatttttcct gcaggatgag cccggtttgg acggtaatat
     4561 gccggagcat gaatgacgac aggtccagca cggatttcat tgcctggatc ctggggaaga
     4621 gcacttcttt cagcaattgg aaatcgcggt gataaccggc cggtaaatta tggatcatga
     4681 gttgcacttc attgggcaag gcggcaatcc gattgcaatg gctccggatc aattcccata
     4741 cgtccgggtt ctttttatgc ggcatgatgc ttgaccccgt ggtcaggtca tcgggaaaag
     4801 atataaatcc aaagtcctgc cccgcgtaaa gacatacgtc cattgccagt ttattgattg
     4861 tggccgccag ggtagccatg gcgaagccca gggccaattc cgttttgccc ctgctcatct
     4921 gggcgttgat aacgttgtaa tgcatgtcct ggaaacccag ggcccgggtc gtcatggtcc
     4981 ggtccagggg gaaagaagat ccataaccgg ctgcagatcc caacggattc tggttggcca
     5041 attgaaaagc agcctgcatg ctgcggaggt catcggccag ggcctcggca taggcgccaa
     5101 accataggcc gaatgaggaa accatggcta cctgcatatg ggtatatccg ggcatcagga
     5161 cttcctggta ttttgtgctc agttcctgga gcaggtcaaa cagggtttgg gtgcccgtca
     5221 ccatctgttc aatctctgcc ctgaaataaa gtttcaaagc caccagcacc tggtcgtttc
     5281 tggaccggcc gctgtgcact tttttgccgg tttcccccaa tctccgggta agttccattt
     5341 caatctggct gtgcacatcc tcggcccccg gttcgattac aaattttccc tgcagggcct
     5401 gatggtagat ctgttgaagt tctttccgga tttgcaggcc ctcgtccttg ctgagcaagc
     5461 cggtggccgc cagcatatcc acgtgtgcga tggaagccag cgcgtcgtat ggagcaagca
     5521 ccaggtccag ttcccggtcg ttgcccaccg tgaaaacctc aaccagctgg tccgtcgatt
     5581 tatttttatc ccaaagtttc atcttaagtc atttattttc cttctcctga agtactaggt
     5641 atcgaattct caaccctcaa ccctcaatcc tcaaccctca atcctcaact ctaaatttcc
     5701 aacccatcca acaattcaat ataaatcctg atcccctcct ttatttcctc aataagtata
     5761 aattcatccg ccgtatggga cctcgcggat tgccccggcc cgatttttaa ggacaggttg
     5821 ggcatcagcg cctggtccga aagggttggg gacccatagg tactcaatcc taaacttaaa
     5881 cccctctgca ccacaggatg gctcaagggg atccccgagg acttcagccg aagcgagcga
     5941 ggggtgagca ccgacttcac cccggattgc aggatatgca gcatctcttc gttggtgtac
     6001 aattcattgc ttcgcacatc gatcacataa tgacaggaat cgggcaccac attgtgttgt
     6061 tttcccgcat ggatgaccgt gacggtggct ttgctcgcgc ccaggtgatc cgatactttt
     6121 tcaaaccggt aattccggat ccattcaata tccggaaggg cctcatagat cgcattgatg
     6181 ccttcgtccc tggcggcatg ccccgattgt cctttggaca cggcatcgat caccatcagg
     6241 cctttttctg caatggccat ttgcatgccg gtgggttctc ccacaatggc cagctgcacc
     6301 ggcccggttt cttccaaaac cctgaggatc ccgttttgcc cggagatttc ttcttccgcc
     6361 gaggcggcga agatcagttg ataggcttgt tgttttttac agagatggga ataggccatg
     6421 gccaggcata ccaccgaagc gcctgcatcg tttgtaccca ggccaaagat tttatcctcc
     6481 ctcaaaaccg gctcatgtgg attaaaggtc caggtttcca cgggacggac cgtatcgata
     6541 tggctgttga gcaggaggac gggcctggat gaatcgtccc gccggcccag gatcagattg
     6601 ttgaggactc tttgtggttg aaggcccagt ttggacaatt cctcctcaag tttatcggct
     6661 ttcgcatgct cttcccggct gtaggccgga atggggatga gcgcctgcag gagggcgatg
     6721 gcctgatccg tcagttggtg gagttcggta tcatcgatgg gcatatctgt gtgcctgaag
     6781 tctggtattg atcgatattc tgggcagcac aaatataaac atcctggatg cctttggccg
     6841 aggctttaaa agcgttttcc agttttggga tcatgccttt ggatatgatg ccggcctctt
     6901 tcaaccggag atatgcttct ttatcgagtt gcgggatgac ggattggtca tcaagctggt
     6961 ccagcagcac tccccttttt tcaaaacaat aaatcagttt gaccgggtat ttttcgacca
     7021 gggcacaggc cagttccgat gccagggtat ccgcattcgt gttgagcagg tggccctggc
     7081 ggtcgtgggt aatggcacaa agcaccggaa gaatgccgtt ttcaagcaaa aaagaccagc
     7141 gtttgataaa aatcttggac acatcaccca cccagccgta atcgatgtcc cttaccggcc
     7201 tcttttgggc ctgtaccagg ttgaggtcgg ccccgctcag cccgagggcg ttgactccca
     7261 tggcttgcag gtcggccacc aggcttttat tggtccagcc ggcataaacc atggtggcga
     7321 tcttgagggt ttcaaaatcg gtgatcctcc ggccgtccac catttttgtt tccataccaa
     7381 gcctggcgga gaggtcggtg gccagctttc ctccgccatg gatcaggatt tttttatgcg
     7441 gaagcccgat aaatttattt aaaaaggaat gggtatcggg cgggtggtcc aatacattcc
     7501 ctccgatttt aatgattaaa acagggtctg atgcgtccat atcggatcaa aggttcatga
     7561 attaatcttt tcccggttta aagatcggaa aattccgggt caacggattt ccggttccca
     7621 caggtaattc cgctggtcaa ccgactttga tacggtattt accggatccc aggcccagtt
     7681 ttaccatccc ttgtccggaa gccagtacct ggatctcctt ttgagccggc aagggctggt
     7741 catccaccat gatattagac gtacccatgg caggaattat aagcgttgag gtcgtattgg
     7801 ccgggatttc aacttcaaag atcaccgctc cattctgttt tttccagccg gagtagatgg
     7861 gtccgtaagg cgtcatggtt gaggccgccg catgctgcaa agaatccgtc aaccggggtt
     7921 gaaggatgct tttcttatac cccggctcct caacctcaat accgcaaacc acccggtaca
     7981 tccagtcgcc gatggcccca taagcataat gattgaatga attcatacct gcggactgga
     8041 aggtggaatc gggtttgatg ccatcccagc gctcccagat cgtagttgcc cccatcttta
     8101 ccgggtaaag ccacgaagga taggtttcct ggagaagcag ggtgtaggcc agatcggtgt
     8161 agccaaacct ggaaagaaca tggcagatgt aaggggtacc cagaaatccg gtagtgatat
     8221 gatggttgta agctctgatg ttgtctgcaa gcctcttcgc cgcatccgcg cgcatagtct
     8281 cgggcaacat atcaaaatga agggccagga cataagcggt ctgggtactt gagaccaggc
     8341 ggccgttggc ggtgacatat tctttccggt aagcttcttt tattttatcc aggaggtctt
     8401 tataataggc gatgtcctcg ttcttaccca atacttcggc tgcccggatc accagctggg
     8461 ttgagtgggc aaagaaacac tgggcgatca gatacttatc ggttacggcc gagcgcccgt
     8521 ccgtatcgtt gccaaggctg taaaacaacc agtccccgaa gtgaaatccc cggttccaga
     8581 ggtattgggt actgctgtcc tgcatatatt tcacccagtt tttcatgctt tcgtactgcc
     8641 tggccagcaa acccttgtct ccatagacct ggtacatatc ccagggaatg atggtcgcgg
     8701 catccgccca tccggcggaa gctaccgcag tagggctcag tacattgggc accacaaaag
     8761 gcaccgcccc atttggcctc tgatctgccg ccaggtcctg gagccatttg gcaaaaaagt
     8821 tctgcactcc gaaattaaaa gccgcggtcc gcgcaaaggc ctgggcgtcc ccggtccagc
     8881 ccagcctttc atccctttgg gggcaatcgg tgggcacatc gacaaaattg cccctttgtc
     8941 cccactgaat attgtgctgc aactggttga ccaggggatt ggaacattca aaatggccgg
     9001 tccgctccat atccgaataa aggacgatcc cggtgaaatt ttcagggttt atggttcccg
     9061 gataacccat gactttaaca taacggaagc cctggaaagt aaaatgaggt tcaaagactt
     9121 ccatccctgc gcctttgaga acaaacttgt tggattgttt tgccctccgg aggttttcga
     9181 tataaaaatt accgaattta tccaggactt ctgcatgaat cagttcaatt tgagtccctg
     9241 cgggcccgct ggctttgact tccacccagc ccgtcatatt ctgcccgaag tccaggacct
     9301 gctctccttt gggggtgacg atcagtttta ccgccttgat cttctccctt ttcctgaccg
     9361 gttcgttata ggtgcctacc aggtttttgg gatggtcttt tagtacgacg gggacccagc
     9421 cggtggccgc gaaatccgga ccagaccagc cggcttgttc tttgcgggcg tcataggtct
     9481 ccccatggta aatttcggaa taacggatgg ggccggtgga ggactgccat gcctggtccg
     9541 agtcaatccg gatcaccatc ccgtttttga gggtgacata caactgactg atcagggcaa
     9601 ggtcttctcc gtagaactta ttgtttttgg taaagccgat ttccccggta aaccaaccgt
     9661 tgcccagggt gacccctatg gcattcatgc ctttttgaag catggacgtg acgtcgtaag
     9721 cctggtattg tattcttttc tgataagccg tccatcccgg cgtcaggtaa tcctgtccta
     9781 ccctctgacc attgatctga gcttcataca atccccgggc gctgataaac aaggtggcag
     9841 atataatatc ctttttaatc tcgaataccc tgcgcagcat agggctgggc ccggccgtat
     9901 cggcggttgg cccggcggct atccaggaag cggtccattc cgcgggatta agcaggcccg
     9961 tatgccagta ctgccccccg gggccggcag ccatattgga atggttgtcc caaacggcca
    10021 gtttccagta ataacgggta ttggagctga gcatagggcc gccataaggg atataggtgg
    10081 attgatctga atcctgttta ctggtccaga gtatgctttt tttattgaag tccggtgcag
    10141 aagccacagt gatttcataa gcagtctgca tggtattttt tgccgggctg gttattttcc
    10201 aactgaaatg gggtttggcg acttccaggc cgaccggatt gaccagttgc tccacccgga
    10261 ggtcatgcac ctgcagttgg gcagaggcaa agacggagat aaatagaaaa acaaaaacaa
    10321 ataaggattt catgatcata ttctggttag cggagtaaaa tattgattaa tcttaatccg
    10381 tgcaaaaatt agttggctac caaattcagg gctaaagccc aaatcagtaa acgtgaacaa
    10441 cccccggcct gaaggccggg gttgggtcaa tgtgtggact aagcccaggc tttagtcaga
    10501 ggtgccatcc acgtcgcctc gcccatggat ggtcaagggt tggactaaac ccaggcttta
    10561 gtcagaggtg ccatccacgt cgcctcgccc atggatggtc aagggttgga ctaaacccag
    10621 gctttagtca gaggtgccat ccacgtcgcc tcgcccatgg atggtcaagg gttggactaa
    10681 acccaggctt tagtcagagg tgccatccac gttgcctcac ccatggatgg tcaagggttg
    10741 gactaacccc aggctttagt cagaggtgcc atccnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnagtcag aggtgccatc cacgtcgcct cgcccatgga tggtcaaggg
    10921 ttggactaac cccaggcttt agtcagaggt gccatccacg tcgcctcgcc catggatggt
    10981 caagggttgg actaacccca ggctttagtc agaggtgcca tccacgttgc ctcacccatg
    11041 gatggtcaag ggttggacta accccaggct ttagcctggg gtttgcacaa aaggataatt
    11101 ttagggcttt agccccttga aggttacaga tgagtcaaaa aactctttcc agctaatctg
    11161 cctctgacct ctgacctcta acccctgacc tctaacctcc gaaaaacttt aaaaaatatt
    11221 ttaatctccc gtaatccttt tgaatgtatt tttgttctta cttagacata gtccaaataa
    11281 cgtttggatg agtattaatt tatatacgac tcaaaatgaa aagagtatat ctcgacaaca
    11341 acgccaccac tccctgtgat cccagggttg tggaagccat gctgccctat tttaccgaaa
    11401 aattcggcaa tgcggccagc cgtagccatc cttatggctg ggaagccgag gaagccgtgg
    11461 actatgcccg ggagcagatt gccaccttaa tccacgccga tcccaaggaa atcatcttca
    11521 cttccggggc aaccgagtcg gataacctgg ctttgaaagg ggttttcgac atgtaccgca
    11581 aaaaaggaaa tcacatcatt accgccacta ccgaacataa ggcggtgctc gatacctgca
    11641 aacacctggc cgaacagggt gccgagatca cctacctcaa ggtgaacgac aaaggcctca
    11701 tcgacctggc cgagctcgag gcggccatca aaccgaccac catcctcgtc tcgatcatgt
    11761 gggccaataa cgaaaccgga gtcattcaac cggtcaaaga aataggcgag atctgcgcaa
    11821 aaaaagggat cctttttatg agcgatgcca cccaggcagt cggcaagatc gatgtgaatc
    11881 ctcgcgaaat gggcatccac ttgatggcct tcactgcgca taaaatgtac gggcccaaag
    11941 gagttggcgc cttgtacgtc agccggaaaa atccccgggt caaggttacg gcccagatgg
    12001 atggcggtgg acatgaaagg ggcatgcgtt caggcaccct taatgtgacc ggcattgtcg
    12061 gttttggcaa agcggccgaa ctcgcccgca aggaaatgga ccaggacatg gcccggatca
    12121 gcaagcttcg cgaccgcctc gaaacgacac tcaaaaacaa actggaagaa gtttatatca
    12181 acggagatcc ggcccaccgc atgccccatg tgaccaatat gtctttcaaa catgtggaag
    12241 gggaaggact gatgatgacc ttcaatcaac gcatggccct ttcatccggt tcagcctgta
    12301 cctccgccag cctggagccc tcctatgtcc tggtcgccct gggactgggt gacgacctgg
    12361 cgcattcctc cctgcgtttc agcctgggca ggttcactac ggatgaagac atcgatattg
    12421 ccatccagtc catcgaggaa ggggtcaacc atatgcgcag cctcagcccg atctgggaaa
    12481 tgtataaaga aggggttgat ctttcccaag taacctggtc accccattaa tcaaaaacaa
    12541 aacctgtatt atttattaaa gtaaattcca atcattatgg catactcaga aaaagtcctc
    12601 gaacacttcc agcatccccg caacgtgggc accctggata aaagcaaaaa caacgtcggc
    12661 acaggcctcg tgggagcccc ggaatgcggc gacgtcatgc gcctccagat cgaagtggac
    12721 gaagagaccg gcacgatcaa agatgccaag ttcaaaacct tcggttgcgg ctcggccatc
    12781 gcttcctctt ccctggccac cgaatggctc aaaggcaaga ccatcgacga tgccctcaaa
    12841 atcgacaata tggatatcgt cgaagaactg gctctgcccc cggtgaaaat ccattgttcc
    12901 gtgcttgcgg aagacgccat caaagccgcc atcaaagatt atcaaaccaa aaacggcatc
    12961 gtggcggaac aaaccgccga agcggccaag gcctgacatt tccttaagca tgatttttgt
    13021 ttccgatacc gctaaaaaac gcatccagga aatcatggcc aaagaacacc tgggcgcgga
    13081 tcattttgtg cgggtcagcg tgaccagtgg cggctgctcg ggcctgtcct ataaaatgga
    13141 ttttgacgat gaaagcaggc ccaatgacca ggtcttcgag gacaatggag tgaaagtggt
    13201 caccgacctg aaaagttttc tatacctgtt taataccacc cttgattttt ccggcggcct
    13261 cagcgggaaa ggattttatt ttgaaaatcc caatgcgtcc cggac
//
LOCUS       DAOQXT010000119        13101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5249_length_13101_cov_6.563084, whole genome shotgun sequence.
ACCESSION   DAOQXT010000119 DAOQXT010000000
VERSION     DAOQXT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13101
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5249_length_13101_cov_6.563084"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1084)
                     /locus_tag="PKM27_16415"
     CDS             complement(242..1084)
                     /locus_tag="PKM27_16415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNR08904.1"
                     /translation="MKNILVPTDFSELSRVAARYAVRLIANQDIRIHLLHVKGVNPAS
                     HALINMHKLETEMDRAAEEDGRDLVRELSALGGNKLKIRLVNKVGNSLRKAIDEYAEK
                     QHIDLIVMGSGGADGLKKALVGSNAVTVINESRIPVIVVPANASFEGLQDIVYATDMN
                     HLATEMDQMSKLARWFGAKLHILHVIRDGKHEKIQVESILATLEKLVPGTHLEFHTAR
                     GSHIAEAIDHFVTHKKADLVALFTHKLEFYEKLFDTGVTRQMAYQNHIPMLAFNKTTH
                     AELN"
     gene            1236..3821
                     /locus_tag="PKM27_16420"
     CDS             1236..3821
                     /locus_tag="PKM27_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNR08905.1"
                     /translation="MNFHLKSIEDCLDHFQSGPQGLSAEEAALRLKEFGPNRLVETKK
                     KPAWLLFLRQFMDFMILVLILAAGISAFLGDLIDTIIILVIVLLNAIVGFVQEYRAEK
                     AIDALKKMATIQAQVLRDGAPVKISSEGLVPGDIVLLQAGNAVPADLRLIESHSLKIN
                     ESALTGESVAVDKSLAPIPGDNIPLGDRLNMAYKSTLVTNGRGKGLVVATAMKTEIGR
                     IAGLLQEEDAATPLQKRMTDFSRKLSYLILLICFLLFGIGLLRGEDPLQMMLLSISLA
                     VAAIPEALPALITIALAKGANRMARQNALIRNLPSVETLGSVNYICSDKTGTLTLNKM
                     TVIHIHKPDGVPETEKDLNYLDLAMALNQDVQEDGNKQQMSGDPTELALLEYFNKMKG
                     PGVYENCVNRFPRVAELPFDSERKCMSTIHRMDEGYLVFTKGAVESITARLHPESPEI
                     PPLADEWSREGLRVLAFAYRILPSLPAGDELDSLEHQMHFLGMAGMIDPPREEAKIAI
                     GQCKTAGIHTVMITGDHPETAAAIARQIGLADGPLQVMHGTELEQLPEQEFARRAEHI
                     QVYARVNPEQKLKIVKALQAGHNYIAMTGDGVNDAPSLKAANIGVAMGINGTDVSKEA
                     ADMILLDDNFATIVKAVREGRRIYDNIRKFVKYILTCNGAEIWTIFLAPLIGLPIPLL
                     AIHILWINLVTDGLPGLALASEKAERDIMTLPPRRAEESLFSGGAGYHIAWVGLLMAG
                     ITLGVQSWAISRELPHWQTMVFTVLSLSQLGHVLAVRSEREFLYSQGLFSNRPLLTAV
                     FFTFLLQLAIIYLPAANEIFRTQALTIKELAICIGLSAIVFHAVELEKWIKQRNRRRP
                     SGGYS"
     gene            3975..4715
                     /locus_tag="PKM27_16425"
     CDS             3975..4715
                     /locus_tag="PKM27_16425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HNR08906.1"
                     /translation="MSLQKLKNAPLKEVIFELHWQQTIELSGFPSDPGFEFAVGRFAE
                     ILKPDFPVHRKLPVADAPIKFIGVPLHQYWKGEMTYPVIQHGQCMLAVNEVEHGYIWS
                     ESFRPLILDSINKLMQSYEGTLNFNKVILKYIDAVDLDEISPHDFISQNLQTRILNDY
                     PIAGKFLDTQLVQAFELEDSSIMRLSIATGKNNKTQKKVVIWTTEVEKNKSLNLDEIY
                     WWIDHAHTHTSDMFKRMLNPEYYASLDR"
     gene            4696..5172
                     /locus_tag="PKM27_16430"
     CDS             4696..5172
                     /locus_tag="PKM27_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08907.1"
                     /translation="MQVSTDSYSINPPIWGASTQNFNNWLPRYLEPDSSTTINWGIPI
                     GINDKIHSLLEEYKNFEANWDGEGTEAPDKKVLICATALLRILDKFGQKVFHASPGPN
                     KEIMLDLRNNNQSKSLEILFYPSRSVYVKFQDANKPIQGEFNFEKLHELMTWLNNA"
     gene            5169..5531
                     /locus_tag="PKM27_16435"
     CDS             5169..5531
                     /locus_tag="PKM27_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08908.1"
                     /translation="MMSERTIVPGDEWLYRRSWPGKKYMNPDGSATSRVFKLREKDNG
                     ELSVNIKSLTTPETTVIDPSQFLLYEIHNKVVEENNLSTWHDPLPENKAHAVIIGMDI
                     DDDIIPGILARRSIRVRI"
     gene            complement(5660..6793)
                     /locus_tag="PKM27_16440"
     CDS             complement(5660..6793)
                     /locus_tag="PKM27_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08909.1"
                     /translation="MNRRLFLPGLFYCLCGLGLLQAQSSARVFPLPKIGDTLLVMQDN
                     LPKTVRHWPFRSDSTRWDLNGVRAPFIRKIMIQNAAADPMALRGANAWVETGFQSKEF
                     YWMDENSIKSIGVTGMDLFGDGRGFTGYWFPQRTVRQAVYTPGILPEARHQLIYPCTL
                     SDLPQTLRDQLPYQPDSIKIMTSVTEQARIQDPVSLDLHLYRHSALRRDLMTVRTTRI
                     ETKKAGLEWQDVTRFVRYPKIFRTDTLRETFFFSPSFPDPVATIRYKNPQEPDQITYK
                     APDIYKDLIVVEDFKPNLFFFPNPYTFGTLRIELLINDPGLYTLRLVNLIGKEVWREN
                     HYLEGNKTMDYSFTHLQKGTYFMIFQQDENHVIGTKRLLVLKP"
     gene            complement(6866..8665)
                     /locus_tag="PKM27_16445"
     CDS             complement(6866..8665)
                     /locus_tag="PKM27_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR08910.1"
                     /translation="MKTLLQFFPYLRPYRNKIFFAALFNLLLAVFTVITIPAFIPFFQ
                     ILFDMNSGPSSGGVLQEIRSFFENLILEEGKSRALVMVCLFLLATLFLKNLFRYLALY
                     VMSPLRSGIIRDIRTRLYEKLMDLPVSFYTNERKGDLMSRMTSDVHEIEWSILTTIEA
                     VFRAPVILLGSLAFMIYVSPALTAIVFVLMIFIGGVIGGISRKLRKQSKAAQDSLGLV
                     SSTLEESIGGMRIIKAFNAEPFMKSRFNEENENYRRISLHMLRRRDLSAPLSEFLGVT
                     AVIVLLYIGSRQVFAREIGPEVFLTFIFAFYSVIEPSKALSQAWFNLQKAGAALDRIN
                     HILKTPVVISSKTGAVEVQEFKEHIQFRNVSFRYHPEGPLVLDHINLTIARGTTVALV
                     GRSGSGKSTLADLLPRFYDVVEGEILIDGINVRDMDLQSLRGLFAMVTQEPVLFHGSI
                     EENILFGQEAGKEEVYEASRLAHAEEFITKSANGYQSQIGDRGLKLSGGQRQRLALAR
                     ALLRKAPILILDEATSALDSESEKLIQLALERVLPDKTAIVIAHRLSTIRKADLIVVI
                     EEGKIVEMGNHERLMATEGPYRRYIELQAQPEA"
     gene            complement(8711..10057)
                     /gene="accC"
                     /locus_tag="PKM27_16450"
     CDS             complement(8711..10057)
                     /gene="accC"
                     /locus_tag="PKM27_16450"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNR08911.1"
                     /translation="MFNKILIANRGEIALRVIRTCKEMGIKTVAVYSTADRDSLHVRF
                     ADEAVCIGPPASAESYLNIPKIISAVEITNADAVHPGYGFLAENARFAEVCTEYGIKF
                     IGPTADQIRAMGDKITAKETMIKSKVPVVPGSDGLLKDFKQATKMAKDIGFPIILKAT
                     AGGGGKGMRIVWEEGELENMWNIARNEAKAAFGNDGIYMEKYIEEPRHIEFQIIGDQH
                     GQVVHLSERDCSIQRRHQKLVEESPSPFMTDKLRKKMGEAAIAAGKSINYEGVGTIEF
                     LVDKYQNFYFMEMNTRIQVEHPVTEEVIDHDLIKEQIKVAAGVKLTGKNYTPQMCSME
                     CRINAEDPFNDFRPSPGKITSFHTPKGYGVRVDTHAFAGYTVPPYYDSMIAKLICRAH
                     TRQELLAKMSRALDEFIVEGVKTTVPFHQLLLNNEDFKAGKFHTGFLNTWDFRSLR"
     gene            complement(10076..10576)
                     /gene="accB"
                     /locus_tag="PKM27_16455"
     CDS             complement(10076..10576)
                     /gene="accB"
                     /locus_tag="PKM27_16455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNR08912.1"
                     /translation="MMDLKEIQELIKMVSKSGLAELKLKRGDFELVLRAESMNKDRAA
                     AGTPVLHPHHIPAHLAPQAPTSPPVVKQEPPAPQLAEPKTEATYLEVKSPMVGTFYRS
                     PSPDKPPYVKVGDVIKPGQVVCMVEAMKLFNEIESELAGTIVKILVEDAAPVEYDQVL
                     FLVEPA"
     gene            complement(10579..11145)
                     /gene="efp"
                     /locus_tag="PKM27_16460"
     CDS             complement(10579..11145)
                     /gene="efp"
                     /locus_tag="PKM27_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNR08913.1"
                     /translation="MAFTTEIRNGMCINHNNDLWSVIEFQHVKSARSAAFVRTKLKSM
                     TTGKTVEYSFPAGTRYDEVRMERRKYQFLYKDDQGYNFMNNENYDQVLIQENMIDGTE
                     FLKEGMDVDILFHAEKETPLSVTLPPQMILEVVYTEPGVRGDTATNAMKPARLETGAE
                     IKVPLFVNEGDKIKVDTQNRSYIERVKS"
     gene            11367..13037
                     /locus_tag="PKM27_16465"
     CDS             11367..13037
                     /locus_tag="PKM27_16465"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNR08914.1"
                     /translation="MRIVLAQINSHIGNFEANLNKILESMQQARSEGADLIVFPELAT
                     CGYPPRDFLEFKDFIHKSREVLERVRLESAGLAVLIGSPAPNPAAQGKDLHNAAFFIH
                     DGKILHQHNKSLLPTYDIFDEYRYFEPSYEFKVFEFKGYRIALTICEDLWNLGNANPL
                     YTVCPLDLMMDQDPDLIFNISASPFAYDHAAERIEVVRANVLKYGKPLFYCNTVGAQT
                     DVVFDGGSLVFSADGRLVDELPYFEEVLQAYEVEKTSGKLEVRRSGDQPKPPAGEQAK
                     EKYSLIHDALLLGIRDYFGKLGFKSAILGLSGGIDSALTATLAVRALGRENVNGLMMP
                     SRFSSKESVEDAVALAKNLGIRYEIIEIEPMYSKFLEQLSPILAGKPFNVTEENIQAR
                     IRGTLLMAASNKEGHILLNTTNKSEMSVGYGTLYGDLCGGLSVLGDVYKTEVYQLVNY
                     INKEREVIPRNSILKPPSAELRPGQKDTDSLPAYEQLDPILYHYIEKRLGPDEIISMG
                     YEPVLVRKILRMVNINEFKREQTAPVIRVSPKAFGMGRRMPIVGKYLG"
BASE COUNT         3240 a         3351 c         3312 g         3198 t
ORIGIN      
        1 gtaatcatcg taataattga cggtatagac ctcgatggaa gaggtgggga aagcctttga
       61 agtataaaaa tggcccgagg tggtcgcagt ttcatacaag gaatccgaaa cagaaggatt
      121 ggaaatggtc gtatgttttc ccgtgaggat cacccgtcct aagatatcgt atttggtgaa
      181 tatccgggag ttattcggat atgggaagtg tcacagttcg aaatcaaacg gggtcagatg
      241 atcagttcaa ctccgcatgc gtcgttttat taaacgccag catcgggatg tggttctgat
      301 aggccatttg tcgggtcacg cccgtatcga ataatttttc ataaaactcg agcttgtgcg
      361 taaacaaggc caccaggtcg gcctttttat gggtcacaaa atgatcgatg gcttccgcga
      421 tatgggatcc ccgggcggta tggaattcaa ggtgggtgcc gggcacgagt ttctccaggg
      481 tggccaggat gctttccacc tgtatttttt cgtgcttgcc gtcgcggatc acatgcagga
      541 tgtgcaattt cgcgccaaac caccgggcca acttgctcat ttgatccatt tccgtggcca
      601 ggtgattcat gtcggtggca tagacgatgt cctgaagtcc ctcgaaggag gcgtttgcgg
      661 gcaccacgat cacgggtatc ctgctctcgt tgatcacggt cacggcattg gaacccacca
      721 gcgctttttt cagtccgtcc gcgcctccgc tgcccatcac gatcaggtcg atatgctgtt
      781 tttcggcgta ttcatcgatg gccttccgga gcgaattgcc caccttgttg accaggcgga
      841 tttttaattt attcccgccc agggcggaaa gctcccggac caggtcccgg ccatcttctt
      901 ctgcggcccg gtccatctcc gtttccagtt tgtgcatatt gatcagggca tggctggcgg
      961 gattcacccc tttcacatgc aggagatgga ttcgtatatc ctgattggcg atcaacctga
     1021 ccgcataccg ggcggccacc cgggacagtt cagaaaagtc tgtgggaacg agtatgtttt
     1081 tcatttttaa acggtataaa catccttatt ccggcaaaaa taccagatga cccgtctcag
     1141 ggcaaacgat gatgaaggtc acggcaagct atgggacttt ggaagacctt gaagaatatc
     1201 gggaatgatg tttattttaa ccatgccagc cgtccatgaa ttttcacctc aaatcgatcg
     1261 aagactgcct cgaccacttc caatccggac ctcaagggtt gagcgctgaa gaagccgccc
     1321 tccggctgaa ggaatttgga cctaaccgcc tggtggaaac caaaaaaaag ccggcctggc
     1381 tgctcttcct cagacagttc atggatttta tgatcctggt cctgatcctg gccgccggta
     1441 tttcggcttt tctgggcgac ctcatcgaca ccatcatcat cctggtcatc gtcctgctca
     1501 atgccatagt gggtttcgtg caggaatacc gcgccgaaaa agccatagat gccctcaaaa
     1561 aaatggccac catccaggcc caggtcctga gggacggcgc gcccgtcaaa atttcttcgg
     1621 agggcctcgt gccgggcgat atcgtcctcc tccaggcagg caacgccgta cccgcagatc
     1681 tgaggttgat cgagtctcac agccttaaaa tcaacgaatc ggcactcacc ggggagtcgg
     1741 tcgccgtgga taaatcgctg gcccccatcc ccggcgacaa catcccgctc ggtgatcgcc
     1801 tcaacatggc ttataaaagc accctggtca ccaatggccg gggcaaaggc ctggtggtcg
     1861 ctaccgccat gaagaccgaa ataggccgga tcgccggcct gctccaggag gaagatgcgg
     1921 ccacccccct tcagaaacgc atgactgatt tcagccggaa gctttcttac ctcatactgc
     1981 tcatctgttt cctgctcttt ggcattggcc tcctgcgggg agaagacccc ctccagatga
     2041 tgctgctttc catcagtctg gccgtggccg ccataccgga agccctgccg gccctgatca
     2101 ccattgccct ggcaaaaggg gccaaccgga tggccaggca aaacgcgctg atccggaacc
     2161 tcccttccgt tgaaaccctg ggttctgtaa attatatctg ctccgacaaa acagggaccc
     2221 tcaccctgaa taaaatgacg gtgatccata tccacaaacc ggatggggtg cccgaaactg
     2281 aaaaggatct gaattatctg gacctggcca tggccctcaa ccaggatgtc caggaagacg
     2341 gaaacaaaca gcagatgagc ggcgatccca ccgaactcgc cctccttgaa tattttaata
     2401 aaatgaaagg acccggggtg tacgaaaact gcgtcaaccg ttttccccgg gtagccgaac
     2461 tcccttttga ttcggaacgg aagtgcatgt ccaccattca ccggatggat gaaggctatc
     2521 tcgtttttac caaaggcgcg gtcgaatcca taacggcccg attgcatcct gaaagcccgg
     2581 aaattccgcc cctggctgat gaatggtccc gggaaggctt gcgggtcctg gcttttgcct
     2641 atcgcatcct gccctccttg ccggcggggg acgaactgga tagcctggag catcaaatgc
     2701 attttttagg aatggccggc atgattgacc cgcccaggga agaggccaaa attgcgatcg
     2761 ggcaatgcaa gacggcgggc atccataccg tgatgattac cggggaccat ccggaaactg
     2821 ctgcggcgat tgcccgccag attggcctgg cggacggccc cctgcaggtc atgcacggca
     2881 ccgagctgga acaacttccc gagcaggaat ttgcgcgtcg cgcggaacac attcaggtct
     2941 atgcgcgggt aaacccggaa caaaaactga aaatcgtaaa agccctgcag gcaggccata
     3001 attatatcgc catgacgggg gatggagtaa acgacgcccc ctcgcttaaa gcggccaata
     3061 tcggggtggc catgggcatc aacggaacag atgtcagcaa ggaagcggcg gacatgattt
     3121 tgctcgacga taatttcgcc acgatcgtca aggccgtccg ggaaggccgg aggatctatg
     3181 acaatatccg gaaatttgta aaatatatcc tgacctgcaa tggggccgag atctggacca
     3241 tcttcctggc cccgctgatc ggcctgccca tccccctcct ggccattcat attttatgga
     3301 tcaacctggt gaccgatgga ttgcccggac tcgccctggc cagtgaaaaa gcggaacgcg
     3361 atatcatgac cctcccgccc cggagggccg aggaaagcct gttttcagga ggcgccgggt
     3421 atcatattgc ctgggtgggc ctgcttatgg cgggcatcac cctgggcgta caatcctggg
     3481 cgatctcccg ggaacttcca cactggcaaa ccatggtctt cacggtcctt tccctttcac
     3541 aactgggtca tgtgcttgcc gtccgcagcg agcgcgaatt cctgtacagc cagggcctct
     3601 tttccaaccg gcccttgctc accgccgtat tttttacttt cctgcttcag cttgcaatta
     3661 tatatctccc ggcggccaat gaaatattcc ggacccaagc cctcaccatt aaagaactgg
     3721 ccatctgtat cggactgtcc gcgatcgtct tccacgcggt ggaactggag aaatggatca
     3781 aacaaagaaa caggaggcga ccatccggtg ggtattctta gaaaaaatga atttttacag
     3841 aaaaagtact ttattgaata tcagccactt accatttaga ggataacttt gaaaatataa
     3901 ccacatatta acaaggaagt tttgcaattt taatcctctt tttaaacaca agctcgcacc
     3961 ttttaaatgt tcttatgagt cttcagaaac tcaaaaatgc cccattaaaa gaagtaatct
     4021 ttgagctaca ttggcaacaa acaattgaat tatccgggtt tccttctgat ccaggttttg
     4081 aatttgctgt cggacgattt gcagaaatcc ttaaacctga tttcccagta catcgaaagt
     4141 taccagtagc ggatgctcct ataaaattta tcggagtacc cttgcaccaa tattggaagg
     4201 gggagatgac ctacccggtc atccaacatg gtcaatgtat gctcgcggtt aatgaagtcg
     4261 aacatggcta catatggagt gaaagtttta gaccattaat tttagacagc attaataaac
     4321 tgatgcaaag ctatgaaggg acattaaatt tcaacaaggt aatccttaaa tacattgatg
     4381 ctgttgattt agatgaaata agtccgcacg acttcataag ccaaaattta caaacccgaa
     4441 tattaaatga ttatccaatt gccggcaaat ttctcgatac acaattggtc caggcctttg
     4501 aattggaaga cagctcaata atgagattaa gcatagccac tggaaaaaat aataaaaccc
     4561 aaaaaaaagt tgtcatttgg accacggagg tagaaaagaa taaatcgtta aacctcgatg
     4621 aaatttattg gtggatagac catgcgcaca cacatacctc tgacatgttt aaaagaatgt
     4681 taaatcctga atattatgca agtctcgaca gatagttata gtattaatcc accaatttgg
     4741 ggagcctcca cccagaattt taataattgg ttgcctcgct atcttgaacc tgattccagc
     4801 actacgatca attggggcat ccccattgga attaatgaca aaattcattc gctactggag
     4861 gaatataaaa attttgaggc caactgggac ggggaaggaa ccgaagcacc tgacaaaaaa
     4921 gttttaatct gtgcaacagc ccttttaagg attctagata aattcggtca gaaggttttt
     4981 catgcttcac caggcccaaa caaggagatc atgttggatt tgcgaaataa caatcagtcc
     5041 aaatccttgg aaattctatt ttatccttcc cggtctgtgt atgtaaaatt tcaggatgcc
     5101 aataagccaa tacaagggga atttaatttt gaaaaattgc atgaattaat gacctggtta
     5161 aataatgcat gatgtctgaa cgtacaatcg taccaggtga tgaatggttg tatcgaagga
     5221 gctggcctgg aaaaaagtat atgaatcctg acggttcggc cacttcacgc gttttcaagc
     5281 ttcgtgagaa agacaatggg gaactttccg taaatataaa aagcctgacc acccccgaaa
     5341 caacggttat cgatccatct caatttttgc tttatgagat tcataataaa gtggtagagg
     5401 aaaacaatct ttccacatgg catgatcctc ttcctgaaaa taaagcccat gccgtcatta
     5461 tcggaatgga tatcgacgac gatattattc ctgggatact tgcccgtcgc tcaataagag
     5521 ttagaattta accagcccca aattatttaa atcaaggaca cccggaatgg gtattttgaa
     5581 gatactccaa agtcgttacc agctcatcga tttaccaaga tttgacggaa atgacgaaat
     5641 ctactggaac tgatatgcct tatggcttca gcaccaataa cctctttgtc ccgatcacat
     5701 gattttcatc ctgttggaaa atcataaaat aggtgccttt ttgcagatgg gtgaaggaat
     5761 aatccatcgt cttgtttcct tccagataat gattttcgcg ccagacctcc ttgccgatga
     5821 gattgaccag gcgaagggta taaaggccgg gatcattgat cagcagctcg atgcggaggg
     5881 tgccgaacgt ataaggattg gggaaaaaaa agaggttggg cttgaaatcc tccactacga
     5941 tgaggtcttt gtaaatgtcc ggggctttgt aggtgatttg gtccggctcc tgcgggttct
     6001 tataccggat ggtggccacc ggatcgggga atgaaggact aaaaaaaaag gtttcgcgga
     6061 gggtgtccgt cctgaagatt ttcgggtaac gtacaaaacg ggtgacatcc tgccattcga
     6121 gacccgcctt tttggtttcg atgcgggtgg tccggaccgt catcaggtca cggcgcaggg
     6181 cggaatgcct gtacaaatgc aaatccaggg ataccgggtc ctggattcgc gcctgctccg
     6241 tcaccgaggt catgatttta atggaatcag gctgatatgg caattggtcc cggagcgtct
     6301 ggggcaggtc cgacaaggta cagggataga tcaattgatg cctggcttcg ggtaaaatcc
     6361 ccggcgtgta aacggcttgc ctgaccgtcc tttgcgggaa ccaatatccg gtaaaaccac
     6421 gcccgtcacc gaacaaatcc attcctgtga ctccaatcga ttttatcgag ttttcatcca
     6481 tccaataaaa ttcctttgac tggaaaccgg tttccaccca ggcgttggcg ccgcgcaaag
     6541 ccatggggtc cgccgcggca ttttggatca tgattttgcg gataaaggga gcccgcaccc
     6601 cattcaggtc ccagcgggtg ctgtcactcc ggaagggcca gtgccttaca gtcttgggca
     6661 ggttgtcctg catgaccagc agggtatccc cgatttttgg cagaggaaat acccgggcgg
     6721 acgactgggc ttgcaggagg cccaagccac aaaggcaata aaacaagccg ggaaggaaaa
     6781 gtcgtcggtt catggaaaac tccatccctg caaacataag ccatggcatg tatttgccct
     6841 tttgtgttaa caatccgttg caaattcagg cttcgggctg ggcctgcaat tcaatatagc
     6901 gtctgtaagg gccctccgtg gccatcaaac gttcatgatt gcccatttcc acgattttgc
     6961 cttcttctat gactacgatg agatcggcct tgcggatagt ggacaggcgg tgggcgatca
     7021 caatggccgt cttatccgga aggacccgct ccagggccag ttggatcagt ttttccgatt
     7081 cggagtccag ggcggatgta gcctcgtcca ggatcaggat tggcgcttta cggagcaagg
     7141 cgcgggccag ggccagcctt tgtctttgcc ccccactcag tttgaggccg cggtccccga
     7201 tctgcgattg atagccgttg gcggatttgg tgataaattc ttcggcatgt gcaagccggg
     7261 aagcctcata tacttcctcc ttaccggctt cctgaccaaa aaggatgttt tcttcaatgg
     7321 acccatggaa caggaccggt tcctgggtca ccatggcaaa aagtccgcgc agggactgaa
     7381 ggtccatatc ccggacgttg atcccgtcga tcaggatctc cccttccacc acatcataaa
     7441 acctgggaag aaggtccgcc agggtggatt tgccggaacc cgacctgccg acgagggcca
     7501 ccgtagtgcc ccgggcaatg gtcagattga tgtggtccag caccaggggg ccttccggat
     7561 gataacgaaa ggaaacattc ctgaactgga tatgttcttt gaattcctgc acctctacgg
     7621 ccccggtttt gctgcttatg acgaccgggg ttttcaggat atggttgatc cggtccaggg
     7681 cggcgcccgc tttttgcagg ttaaaccagg cctgggacaa tgccttggag ggctcgatca
     7741 cactgtaaaa agcgaagata aaagtgagga agacttcagg gccgatctcc cgggcgaata
     7801 cctggcggct gccgatataa agcaggacaa tgacggcggt cacccccagg aactcggaaa
     7861 gcggcgccga aaggtccctt ctccggagca tgtggaggct gatccggcgg tagttttcat
     7921 tttcttcatt gaagcgggat ttcatgaacg gctccgcgtt gaaggctttg atgatgcgca
     7981 tgcccccgat cgattcctcc agggtgctgc tgaccaggcc aagcgaatcc tgggccgctt
     8041 tggattgttt tctgagctta cgcgaaatcc cgccgatcac cccgccgata aaaatcatca
     8101 ggacaaaaac gatggcggtc agggccgggc tcacatagat cataaaggcg aggcttccaa
     8161 gcaggatcac cggcgcgcgg aagacggctt cgatcgtggt caggatggac cattctattt
     8221 catggacatc gctggtcatc ctcgacatca ggtccccctt tcgttcattg gtataaaacg
     8281 agaccggcag gtccatcagt ttttcataaa gccgggtgcg gatatcccgg atgatcccgg
     8341 accgcagcgg gctcatcacg tacagggcca ggtagcggaa caggtttttt aaaaacaggg
     8401 tagccagtaa aaaaaggcag accatgacca gggccctgga tttgccctcc tccaggatga
     8461 ggttttcaaa aaacgaccgt atttcctgca ggacccctcc ggaagaaggt ccggaattca
     8521 tgtcaaaaag gatctggaaa aagggaataa aggcggggat ggtaatcacg gtaaacaccg
     8581 cgagcagcag attaaagagg gctgcgaaaa aaattttatt ccggtaaggt ctgaggtagg
     8641 gaaagaattg caacagggtt ttcattacat caggaccgct tgatgcccgt gtcgggcagg
     8701 gtgagccgga ttatcgtaaa ctcctgaaat cccaggtgtt taaaaaaccg gtgtgaaatt
     8761 ttccagcttt aaagtcttcg ttattgagca gaagctggtg aaaaggtacg gtcgtcttca
     8821 ccccttccac gataaattca tccagggccc ggctcatttt agccagcaat tcctggcggg
     8881 tatgggcgcg gcagatcagt ttggcgatca tggaatcgta atacggcggg acggtgtatc
     8941 ccgcgaaggc atgggtatcc acccttaccc catacccttt tggagtgtgg aaggaggtga
     9001 ttttacccgg gctgggcctg aaatcattga aggggtcctc cgcgttgatg cggcattcca
     9061 tggagcacat ctggggcgtg taatttttcc cggtcagttt gacgcccgcc gccactttga
     9121 tctgttcctt gatgaggtcg tgatcgatca cttcctcggt cacgggatgc tctacctgga
     9181 tccgggtatt catctccatg aaataaaagt tctggtattt atccacaaga aactcgatgg
     9241 tccctacccc ttcataattg atggatttac cagccgcgat cgcggcttcc cccatttttt
     9301 tccggagttt atcggtcata aagggggaag gggattcttc cacgagtttc tgatgcctgc
     9361 gctggatgga gcagtccctc tcggagagat gcaccacctg cccgtgctgg tcgccgatga
     9421 tctgaaattc gatatgccgg ggttcttcga tgtatttttc catgtatatg ccgtcattgc
     9481 caaatgccgc tttggcttca ttccgggcga tattccacat gttttccagt tccccttcct
     9541 cccatacaat gcgcatgccc tttcccccgc cgccggcggt cgctttgaga atgatgggga
     9601 aaccaatgtc tttggccatt ttggtggcct gcttgaaatc tttcaaaagg ccgtcgctgc
     9661 cggggactac gggcaccttt gatttgatca tggtttcctt cgctgtgatc ttatcaccca
     9721 tggcccggat ctggtcagca gtgggaccga tgaatttaat gccgtattcg gtgcagactt
     9781 cggcaaaacg ggcgttctct gcaagaaatc cataaccggg atggacggcg tccgcattgg
     9841 tgatttcgac ggctgaaatg atcttgggta tgtttaaata ggattcggcg cttgcggggg
     9901 gcccgataca gaccgcttca tccgcaaacc gtacatgcag gctgtcgcgg tcggcggtcg
     9961 agtaaaccgc cacggtcttg atgcccatct ccttacaggt gcggatcacc cgcaaagcaa
    10021 tttcaccccg gttggctatc aatattttat taaacatatg gtctcactaa gggcttcagg
    10081 caggttcgac taaaaatagg acctggtcgt attccaccgg ggccgcatct tccaccagga
    10141 ttttgacgat ggtgccggct aattccgatt cgatttcatt gaacagtttc atggcttcca
    10201 ccatgcacac cacctggccc ggtttgatga catcgcccac cttgacatag ggaggtttat
    10261 ccggggaggg ggaacggtaa aaagtgccga ccatcgggga tttgacctcg aggtaagtcg
    10321 cttccgtttt tggttctgca agctgggggg ctggtggctc ttgttttaca accggggggc
    10381 tagtcggtgc ctggggtgcc aggtgcgccg ggatatggtg gggatgcagg accggggttc
    10441 cggctgcagc cctgtccttg ttcatggact ctgccctcag gaccaattca aaatcacctc
    10501 ttttcaattt caactccgca aggcccgatt tgctcaccat tttgatgagc tcctggattt
    10561 ctttgagatc catcattttt aactttttac ccgttcaata tacgacctat tttgggtatc
    10621 gactttgatt ttgtcccctt cattcacgaa gagcgggacc ttgatttccg cgccggtttc
    10681 caacctggcg ggtttcatgg cgttggtagc cgtatcgccc cggaccccgg gttctgtata
    10741 gacgacctcg aggatcattt gagggggcag ggttacgctc agcggggttt ccttttcagc
    10801 atgaaaaagg atatccacat ccatcccttc cttgaggaat tccgtaccat cgatcatgtt
    10861 ttcctggatc aggacctggt cgtagttctc gttgttcata aaattataac cctggtcgtc
    10921 cttgtaaagg aactggtact tcctcctttc catacgcacc tcatcataac gggtgccggc
    10981 cgggaaagaa tactccacgg tcttgcccgt cgtcatgctc ttcaatttcg tccggacgaa
    11041 agccgcactc cgggcgctct taacatgctg aaattcaata acagaccaca aatcgttgtt
    11101 gtgattgatg cacatcccgt tgcgtatctc cgtggtaaaa gccatatctt ttttaatttt
    11161 tgagggcgca aagataggaa tttagtttgt cagttgacca gtttgtaagt taaccagttt
    11221 gtcatccgcc tagggtgcat gtcaaatact cgaaacttgg acctcaccgc ccctaaattc
    11281 tatttcccag cttctaactt gccaacttgc caacttacta acttgccaac ttactaactt
    11341 gctaactaac tccctacctt tgccccatgc gcatcgtgct cgcccagatc aattcccaca
    11401 ttggcaattt tgaggcaaac ctgaataaaa tcctggaatc catgcagcag gcgagatcgg
    11461 agggagccga cctgattgta ttcccggaat tagctacctg cgggtatccg ccccgtgatt
    11521 tcctggaatt caaggacttc atccataagt cccgggaggt tttggaaagg gtgagactgg
    11581 aatccgccgg tctggcggtc ctgatcggat ctcccgcccc aaatcccgcg gcccagggca
    11641 aagaccttca caacgccgct tttttcatcc acgacggaaa gatcctccac cagcacaaca
    11701 aaagcctgct gcccacttat gacatatttg atgagtaccg ttatttcgaa cccagttatg
    11761 aatttaaggt ctttgaattc aaaggctacc ggatagccct caccatctgc gaagacctct
    11821 ggaacctggg caatgcaaac ccgctctata cggtctgccc cctggacctg atgatggatc
    11881 aggaccccga cctcatcttt aatatctccg cctccccctt tgcttacgat catgcggcgg
    11941 aaaggattga agtggtgagg gccaacgtcc tgaaatacgg gaaacctctt ttttattgca
    12001 ataccgtggg tgctcagacc gatgtggtct tcgacggggg atcccttgtg ttttctgccg
    12061 atggccgcct ggttgatgaa ttgccctatt ttgaagaagt cctgcaggcc tatgaggtgg
    12121 agaagacgtc cggaaaactc gaggtgcgga gatccgggga tcagcccaaa cccccggccg
    12181 gtgaacaagc taaagaaaag tatagcttaa tccacgacgc cctgctgctg gggatcaggg
    12241 attattttgg aaaactgggt tttaaatcgg ccatcctagg gctcagcgga ggcattgatt
    12301 ccgcgctcac ggccaccctt gcagtccggg cgcttgggag ggaaaatgta aacggattga
    12361 tgatgccgtc ccggttcagc agcaaggaga gcgtggagga cgccgtggct cttgccaaaa
    12421 acctgggcat ccgctatgaa atcatcgaaa tagagcccat gtattcaaaa ttcctcgaac
    12481 aactgtcgcc catccttgcc ggcaaacctt ttaatgtcac cgaggaaaac atccaggccc
    12541 gcatccgggg aacgctgctg atggccgctt caaacaagga ggggcatatc ctgctcaata
    12601 ccaccaataa aagcgaaatg tcggtgggtt acggcaccct ttatggagac ctttgcggcg
    12661 gcttatcggt gctgggcgat gtctataaaa cggaagttta ccaacttgta aattatatca
    12721 acaaagaaag ggaagtcatt ccccggaatt ccatccttaa gcccccgtcc gcggaactca
    12781 ggcccggaca aaaagacacc gacagccttc ctgcctatga gcaacttgat ccaatccttt
    12841 atcattacat tgaaaaaagg ctcggccccg atgaaatcat ttccatggga tacgaacccg
    12901 tcctggtcag gaaaatttta cggatggtaa acatcaacga attcaaaagg gaacagacgg
    12961 cgcccgtgat ccgggtgagc cccaaagctt ttggcatggg cagaagaatg cctattgtag
    13021 ggaaatacct ggggtaagag tcaagagtta atagtcaaga gtcaagagtc aataatcaat
    13081 agtcagaata ccaacagcta g
//
LOCUS       DAOQXT010000120        13078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5257_length_13078_cov_6.254012, whole genome shotgun sequence.
ACCESSION   DAOQXT010000120 DAOQXT010000000
VERSION     DAOQXT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13078
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_13078_cov_6.254012"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..606
                     /locus_tag="PKM27_16470"
     CDS             97..606
                     /locus_tag="PKM27_16470"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNR08915.1"
                     /translation="MNLSLLRQACCILVSLFLFIACNQSRHIISSHAEKDREFSQLRG
                     KKIDQLLLAAGGVLGKAYRLGSAGPRAFDCSGLTRFVFDKINMTLPRKAKDQAETGTA
                     IPLKLVREGDLLFFGHRRVHHVAIVSKVKRGNIYITHATTSRGVVEEKLEDSEYWSSK
                     LKMARRVIM"
     gene            825..1418
                     /locus_tag="PKM27_16475"
     CDS             825..1418
                     /locus_tag="PKM27_16475"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNR08916.1"
                     /translation="MINLILFGPPGSGKGTQAQRLVEKYDIAHWSTGDMFRFELSNHT
                     ELGQLAKSYMDKGQLVPDEVTISMLKKRMESGKATNGFILDGFPRNIVQAQALDALLE
                     EKNLKITALLSLEVDEEELINRLLNRGKVSGRADDINEEIIRRRLQVYKEETTPVYNY
                     YAEKQLAVQVNGMGTIDEIFEELCLLIDDRYTTQFVY"
     gene            1431..2426
                     /gene="obgE"
                     /locus_tag="PKM27_16480"
     CDS             1431..2426
                     /gene="obgE"
                     /locus_tag="PKM27_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNR08917.1"
                     /translation="MAEENFIDFVKIQCKSGKGGAGSIHFHRGKYIPRGGPDGGDGGK
                     GGNIIVTGNNQLWTLLHLRYTKHLFAGDGKPGSKANQTGHGGEDMEIQVPLGTVVKSV
                     ETGETLAEITHHGEQKTILEGGRGGKGNAFFKSATHQAPRFAQPGEPGVEAWLSFELK
                     ILADVGLVGFPNAGKSTLLAALTAAKPKIASYPFTTLVPNLGIVRYRDDKSFVMADIP
                     GIIEGAHEGRGLGLRFLRHIERNAVLLFIVPSDSENISKEFKILVNELNQYSPELMDK
                     PRLLAISKMDLVPEGQSASIKRKIPKGVPYCLISAASGQGLDELRDLLWKNIQGS"
     gene            complement(2512..3180)
                     /locus_tag="PKM27_16485"
     CDS             complement(2512..3180)
                     /locus_tag="PKM27_16485"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNR08918.1"
                     /translation="MRRTPFLYSFYFISLFHLLALVAAWEPAVFYSKPLLMPSLLLAL
                     IKSTGRLHGPFVAAAWALAFSTGGDVLLMMEKNAGPLFFYFGLGSFLIAQLFYIRAFL
                     QLGTIRIASTWSLLYALYYLVFIGSLFKAMDLPLLIAVALYGLCLTTMAWNSWRTSGG
                     NLMLSNLGVAGAILFVASDSLIAINRFAMPVPSAGFLIMSTYILAQYLIAEGISRKAA
                     LSKG"
     gene            complement(3180..3710)
                     /locus_tag="PKM27_16490"
     CDS             complement(3180..3710)
                     /locus_tag="PKM27_16490"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNR08919.1"
                     /translation="MTLDILAAAVAAYGFYLGFTKGIVKTLFGFVAVFIGLIAALKLS
                     PYLVKALEKVSGNDSPLIFLVGFALTFVLTIFLIRFMGNRFEDLLKAVKINFFNQLVG
                     GVALSLLFLLLFSYGVWFANQVKVIPESSKKASYSYHYLEKLPERSRLLFSKLKPLFS
                     EFSSKATETIERVKNQ"
     gene            3871..4365
                     /locus_tag="PKM27_16495"
     CDS             3871..4365
                     /locus_tag="PKM27_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08920.1"
                     /translation="MGLLIWLMGFSLSGTMQAAGRPGTGPVDTLPVVFSIGQYSDEAE
                     KLNLSHISLMEVCNDEVLLAYDKWLHMLAELNDEAADFGLDLKGTKFWFSVYWHHTGR
                     IKHFAYYPKPGSKPIDYKVFEDLLSHFIDSYTLPVSSPEDFYNYGSAAFPIPSYRKPV
                     ARQN"
     gene            4546..5148
                     /locus_tag="PKM27_16500"
     CDS             4546..5148
                     /locus_tag="PKM27_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR08921.1"
                     /translation="MDDHTLIREGLRTLLDQIDHFDWIAEFNHPSALMEALRARMPVD
                     IVLIDVFYNRENRMHDIGRMASQYLGIKWMILSAYESQALVQQAFSLGISAYLRKDVT
                     LEELKHALQRTWAGKKNLNYTGVSDGPAFAPQGDTEPLSVREKEIIGLIVKGRTEQQI
                     AESLFISKHTVHTHRKNILKKLGLHSNADILKYFIENNLG"
     gene            complement(5140..7005)
                     /locus_tag="PKM27_16505"
     CDS             complement(5140..7005)
                     /locus_tag="PKM27_16505"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNR08922.1"
                     /translation="MFRALFPGLVLILGNLRVCASNSLDWANRQLALSLEVYEDRNGS
                     LEFQDVGHLPPSAFRAGSVTQNFGFTLSAWWLRFHLHNPEEREEKFYLRLANGHIHSV
                     DFWLVRDGQAEQVHTGNALLSSTKPVRYTEYLFPVELPAGQSCTIYLRLEKKGAELYA
                     PLSILRGDYDPAGNFNTWNLLFGAAFLYILILFIASCIIRTRLIVFYFLYAASLCLYI
                     ATVKGIAPAMIWPNQPWIQINALELSKHLANIFYILFILKFIGKDHRSSTLIGFLKIC
                     IGLSALNLVCRLIYSTLGVIPDALMMRFVQLTALLLPAAAVCLAILLYRAWRTNKKSE
                     EFWLLFITTALLVPLAFLVCLHFGWIPAYPYYSNLLAFLFLVEIAGISAILVYRYYQL
                     YRKELFHLREITRLKRKAVENILLGQEEERIRIARDLHDGLGMSLAHIRMNLSLLDQY
                     VGPGRGKGLLNGLLGSVGDAAKEVRGISHNLAPLSLQHQELTKALEELVYRIELTDAT
                     LDIELHFAPDINTSLSPIVKQNIYQTAKELFNNIVKYASASQIRVRLHRDQDHYKLEL
                     EDNGMPYDLEKAMAESGGMGLSSIRSRATLLNGWFIALPGRDGGMIHQFSIPVSA"
     gene            7228..8133
                     /locus_tag="PKM27_16510"
     CDS             7228..8133
                     /locus_tag="PKM27_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08923.1"
                     /translation="MKMKYRFRIKWSKVLFALLSFGRAWLPAQTIDSYIGTYSGENKN
                     GYLRPVASVISSAFNTGQVTHTKLDSNFRVYFHLIGTASFILGDGLKYFDGTTPEGFQ
                     PSKTISAPTLLGPRESVTVEGINGTAYTFPAGMGIRMLSLVVPQLSLAYGKTELNFRF
                     AWGDTDDEIGRINYWGLGLRHDLGQYLMPDAPWLLSVGGMYQKIKVGTYANLSAYNAS
                     LTAGQEWRHVYYFFQAGYQAGNLKSQYNYFNGENKEVVKVDLSSDNPLFIGAGLGLKL
                     GFLQLQWQVQGYEPVIGSFALGFRF"
     gene            8151..8777
                     /locus_tag="PKM27_16515"
     CDS             8151..8777
                     /locus_tag="PKM27_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08924.1"
                     /translation="MKSLFFLSLLTVAWSSCNYITLRRHLNVNVDDVKTYDQSGAFEK
                     SYFINTEKIINAVTENVNTEGGSIEKLEIESIQITVGLLPNNTASSVHNVHVRLGNGF
                     VQAGESLVKLDTSRLPINSATVFLVNNFMIFKGVSILKTELVKKLATGQFEFFELKLA
                     GTIPAGQVFRGSMKLIIKASMDVVTCERVPIGLGPSECMIKTMVLPFD"
     gene            complement(8812..10812)
                     /locus_tag="PKM27_16520"
     CDS             complement(8812..10812)
                     /locus_tag="PKM27_16520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08925.1"
                     /translation="MKTILAFLTSTSTLTLTLTSTLTCPELRAQKPALHQVRVDPDGS
                     FTPRYIHLNALDTVEWLFSNRNQSVAPITLADSLSLQCDQFKPRVFADPNDFTGPAAS
                     LMPGIVALSPDGPGFEILSTGQNCPQNLLKAAAGNQFLCERGPDYATMDWTWKNPNIT
                     GVFIRLRWDEVHLGPGKYDWTFLDREITKAVENGKMYSLGFKAGLTGTPAWIFNPSVA
                     GNFTVKKLRLRDNEDEETCGTPGDMGSPSDVHYRKWYFDLWRDVAAHIKEKNAWYRAL
                     GYVKPSGMNLFTHENRLPKNCKDGCEVCNPEVWAKGGYTPNALYDFYTQQFNLLDTLF
                     PDKYMQYALIQAGFPLVNNKGEYTAPFTEPLPDGTEQTEKIMALGRTQHGLKFIVSHN
                     GLGPRPQDGNQPLAPCPNEGKHPAVRPFAQAATRCPNPYALREGEAGQITGWQSQNAR
                     TVNSPETMESTLRNAWDNSDGIYLEFYEERIWEAETAGPVLDPKGTGRTLRQWDSLLH
                     QRVKSFWGTKVEDPFPLKHRQVFKRTTANKNEAQVFYYVNPQSCKNGTKSFGVITLRA
                     ESSVPYGTEPEEEEQIQLALYPNPGIGPTFFRYFLPRPVEVRLEVYDPLGRRIAGLVR
                     DTRPSGWNEYIWPDNFQLAPGTYMVRLNAGKQTLTRRLMLMR"
     gene            complement(10878..11291)
                     /locus_tag="PKM27_16525"
     CDS             complement(10878..11291)
                     /locus_tag="PKM27_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08926.1"
                     /translation="MKNLLLFIGGLFLFFISCSPKPVAVAAIADGGWRFLGDKWVNFG
                     ADHDELFLGNIKDDFRQIRLRVTDGPLHVMDMKVHFDNGAVQDVPLRSVIRQGQQSRV
                     IDLEGGLRHLQKITFWYETVGYRKGRSRVAVWAYR"
     gene            complement(11416..12153)
                     /locus_tag="PKM27_16530"
     CDS             complement(11416..12153)
                     /locus_tag="PKM27_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNR08927.1"
                     /translation="MPLNPVLPLYTIAISQSGNCVTCPFRKFSLFDGLETKYLEILKS
                     HKSIVTYRAGEILYKEGNKIQGLICLKSGKLKEFKTAPSGNKLILSLKKPVEFVGLPD
                     LLLHSNHQTSVMALEDAEICIVEKEDFMQVFRNNSLFASRVSIYLARLLEESQNHLLQ
                     LTQKHMRGRLAYALLYLREYFGERPVDHFIDSTLKRSDLAALTNLTTANVIRTLSSFS
                     EESLIELHGRGIRILDHARIQQICELN"
     gene            complement(12330..>13078)
                     /locus_tag="PKM27_16535"
     CDS             complement(12330..>13078)
                     /locus_tag="PKM27_16535"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNR08928.1"
                     /translation="GGRFPFGGRFRTFATSEAQKLCPPPLAYSENPTSAFYAQALEVF
                     TLSNAGMPYEDRWISEFWSDDIEGQTFSPPSRPIAILNEIMVSEKINLEKAVESVAKI
                     GLALNDFAVACWHSKWVYNVVRPETYIQKLFDPKWEPILTNALSGDQGLTPPFPAYPS
                     GHSTFAGGSGVILAHLFGSNYEFTDLCHKGRIEFLGTPRTFNSFEDAMKENALSRITL
                     GVHFRMDCDAGLTLGQQTARRILKLPWKVQ"
BASE COUNT         3394 a         3129 c         3377 g         3178 t
ORIGIN      
        1 gttaaactat tgttatttaa ttagttatat agtttaacat ccattaacat catattaaaa
       61 gaaggcagga aacaagggtt ctatttttgc ctgtccatga acctctcctt attgcgccag
      121 gcatgctgta tccttgtcag cctttttctg tttatcgcat gtaatcaaag ccgtcacatc
      181 atatcctctc acgcggaaaa ggaccgtgaa ttttcacaac tcagggggaa aaaaatcgat
      241 cagttgctcc ttgctgcggg aggggtgctg ggaaaggcat accgcctcgg cagcgcgggc
      301 ccgagggcct ttgattgttc ggggctgacc cgttttgtat ttgataaaat caatatgacc
      361 ttgccgcgaa aagccaagga ccaggccgaa accggtacag ccattccatt gaagctggtc
      421 cgcgaaggcg atcttttgtt tttcgggcat cgccgggtcc atcatgtcgc catcgtcagc
      481 aaggtaaaaa ggggaaatat ttatattacc catgccacca ccagccgcgg ggtagtcgag
      541 gagaaactcg aagattctga atattggtct tcaaaattga agatggccag gcgagtgatc
      601 atgtagaatc aatagtcagc agtcagtagt cagtagtcaa tagtcaatag tcagcagtca
      661 gtagtcagcc atcagcagtc agccatcagc agtcagatcc tgatgctttc ggaacttccg
      721 ggcatcggtc agcggtaagc ctactgctga aaactgagag ctggctgctg aaagctccaa
      781 ttctttgtat ctttcgccgt attgaaacga tctaccttga agagatgatt aatttaattt
      841 tatttggccc tcccggatct ggcaaaggca ctcaggccca gcgtttggtg gaaaaatacg
      901 acattgccca ttggtccaca ggcgatatgt tccgttttga attgagtaat cataccgaac
      961 ttggccaact ggccaaatcc tatatggata aaggccaact ggtgccggat gaagtgacca
     1021 tctccatgct gaaaaagcgg atggaaagcg gcaaggccac caatggcttt atcctggatg
     1081 gctttccgag gaatattgtc caggcccagg ccctggatgc cctgctggaa gaaaaaaacc
     1141 tgaagatcac cgccctgctt tcactggaag tcgatgaaga agaactgatc aaccgcttgc
     1201 ttaaccgggg aaaggtatcg ggccgggcgg acgatatcaa tgaagaaatt atccgccggc
     1261 ggctccaggt ctataaggag gagaccaccc cggtgtacaa ttattacgca gaaaaacaat
     1321 tggccgtaca ggtcaacggc atgggcacga tagacgaaat cttcgaagag ttgtgcctgc
     1381 tcatcgacga tcgatatacg acccagtttg tatattgata cctgcagagc atggctgaag
     1441 aaaattttat tgattttgta aaaatccaat gcaagtctgg taaaggaggc gcgggctcca
     1501 ttcatttcca ccggggcaaa tacatcccca ggggagggcc tgacggagga gatggaggga
     1561 agggcggaaa tatcatcgtc accggcaaca accagttgtg gacccttctt cacctcagat
     1621 ataccaagca cctttttgca ggcgacggca agcccgggag caaagccaac cagaccggcc
     1681 atggcgggga ggatatggaa atccaggtcc cgctcggaac cgttgtaaaa agtgtggaga
     1741 ccggagaaac cctggccgag attacccacc atggagaaca aaaaaccatt ctcgaagggg
     1801 gtaggggggg caagggcaat gcctttttta aatccgccac ccatcaggca ccccgctttg
     1861 cccagcccgg tgaaccgggt gtggaagcct ggttgtcttt cgaactgaaa atcctggccg
     1921 acgtgggcct ggtaggattt cccaatgccg ggaaatcaac tctgctcgcc gctcttaccg
     1981 cggccaaacc taaaattgcc tcctatccct tcaccaccct ggtccctaat ctcgggattg
     2041 tccgttaccg ggatgacaaa tcctttgtaa tggctgatat ccccggaatc atcgagggtg
     2101 cccacgaagg aagaggactg ggcctgcgat ttcttcgtca catagaaaga aatgcggtac
     2161 tcctgtttat tgtcccttca gacagcgaaa acatcagcaa ggaatttaag atcctggtca
     2221 atgaactgaa tcaatacagc cccgaattga tggataagcc ccgcctgctc gccatcagta
     2281 aaatggacct ggtccccgaa ggccagtcgg cttctatcaa acgaaaaatt cccaaaggag
     2341 tgccttattg cctgatctcc gccgccagcg ggcagggact cgatgagctt cgggatctgc
     2401 tgtggaagaa tattcaggga agttagaagt tgggagctcg aagctcgagg ctggaagctg
     2461 ggatgccggg gaataagctg ttaaaatgca ggcctcaaac cattcaaccg tttatccttt
     2521 cgacaatgcc gcctttctcg atatgccttc cgcgatcagg tattgggcca gtatataggt
     2581 cgacatgatc aaaaaaccgg ctgaagggac cggcatggca aaacggttga tggcgatcag
     2641 gctgtccgaa gcaacgaata gaatggcccc ggccacgccc aggttggaca acatgaggtt
     2701 gccgccggag gtccgccagc tgttccaggc catagtcgtc aggcaaaggc cgtagagggc
     2761 taccgcgatc aataaaggca gatccatggc cttaaaaaga gatccgataa aaaccaggta
     2821 ataaagggcg taaagcaggc tccatgtgct ggcaatccgg atagttccca actggagaaa
     2881 agcccggata tagaaaagct gggcgattaa aaaggaaccc aggccaaaat aaaaaaagag
     2941 gggacccgcg tttttctcca tcatcagcag gacatcaccg cccgtgctga acgcaagggc
     3001 ccaggctgcc gcaacaaagg gaccatgaag ccggccggtg cttttgatca atgccagcaa
     3061 gagggagggc atcagcaagg gtttcgaata aaaaacggcc ggctcccagg ctgccaccag
     3121 ggccaggaga tggaacaggc tgatgaaata aaaactatat aaaaaggggg tccttcgcac
     3181 tattgatttt ttaccctttc gatggtttca gtcgctttgg aggaaaactc gctaaaaagg
     3241 ggtttcagtt ttgagaataa caagcgggat ctttccggga gtttttcgag ataatgataa
     3301 ctgtaactgg ccttcttgga ggattcgggg atgactttga cctgattggc aaaccagacc
     3361 ccgtaagaaa acaaaagcag aaagagaagg cttaaggcca ctccgccgac cagttggttg
     3421 aaaaaattga ttttgaccgc tttcagcagg tcttcgaacc gattgcccat aaaccggatc
     3481 aggaaaatgg tcaatacgaa ggtgagggca aatccgacca ggaaaatcag ggggctgtca
     3541 ttgcccgaaa ctttttcaag ggccttgacc aggtagggcg acagtttgag agcggcaatc
     3601 aaaccgatga acacagccac aaaaccaaac aaggttttga cgatcccctt ggtaaaaccc
     3661 aggtaaaagc cgtacgcggc caccgcggca gccaggatat ctaaggtcat gttccgctgg
     3721 tattaatttt tgaaatcaag cccaatattc gtttgattca acctgcccgt ttcttttttt
     3781 cgaccaatgg accgaaatat atgttaaaat tataatatag gaatagtttt tgtaatttcc
     3841 cttatccatc ttatttttaa ttggatgaga atggggctgc ttatatggct aatgggtttt
     3901 tccctatccg gcacaatgca ggctgcggga aggcccggga ccggtccggt cgataccctt
     3961 cctgtggtat tcagcattgg ccaatacagc gatgaagcgg aaaaactcaa tctgtcccat
     4021 atttccctga tggaagtatg caatgacgaa gtgctgttgg cttacgacaa atggttgcat
     4081 atgctcgcag aactgaatga cgaagccgcc gatttcggct tggaccttaa gggcactaaa
     4141 ttctggtttt ccgtgtattg gcaccatacc ggccgtatca aacattttgc ctattatccc
     4201 aagcccggat caaaacccat tgattacaaa gtcttcgaag atttgttgtc gcatttcatc
     4261 gattcatata cgctgccggt atcttccccg gaggactttt acaactatgg cagcgcagct
     4321 tttccaattc cctcataccg taaaccggtg gccaggcaaa actgatcgcc ggatctcgat
     4381 ttgtttacgc cctgaaacgc ctgaggtgtt caatgctgac ttccgaattg gaggggaagg
     4441 gtcatccggt gggaggacgg tacagtgctt ttggatacga gtggccggcc tgtaaatgct
     4501 gatttatttt tcttacatta gagtattgtg ccaaatgtag tctgtgtgga tgatcatacc
     4561 ctcatccggg aaggcctgcg caccctgctg gatcagattg accatttcga ctggatcgcc
     4621 gaatttaatc acccttcggc gcttatggag gccctccggg ccaggatgcc cgttgatatc
     4681 gtactcatcg atgtttttta taaccgggaa aacaggatgc acgacatcgg gcggatggcc
     4741 agccaatacc ttgggatcaa atggatgata ttgagtgcct atgagtccca ggccctggtg
     4801 caacaagcct tttcgttggg gatcagcgct tatttaagga aagatgtcac cctggaagag
     4861 ctcaaacatg cccttcagcg gacctgggca ggcaaaaaga acctgaatta caccggggtc
     4921 tcggacggac ctgcctttgc tccccaggga gataccgagc ccttgtctgt aagggaaaaa
     4981 gagatcatcg gccttattgt caaagggagg accgagcagc aaattgccga atctttgttc
     5041 atcagtaaac ataccgttca tacccacagg aaaaacatcc ttaaaaaact cgggcttcat
     5101 tccaatgcgg atattctaaa atatttcatc gaaaacaacc taggctgata ccggaatgct
     5161 gaactggtga atcatgcccc cgtcccgccc cggaagggca atgaaccatc cgttgagtaa
     5221 agtagcccgc gaacggatac tggacaatcc catccctccg gattcggcca tggccttttc
     5281 aagatcataa ggcatcccgt tgtcttccaa ttcaagcttg taatggtcct ggtcacggtg
     5341 aagccggaca cggatttggg aagcgcttgc gtatttcacg atgttgttga acaattcctt
     5401 tgccgtctga tagatatttt gttttacaat ggggctcagg ctggtattga tatccggcgc
     5461 aaaatgcaat tcgatatcca gggttgcatc cgtgagctcg atccgataga ccaattcttc
     5521 cagggctttg gttaactcct ggtgctgcag ggacagggga gccagattat gggaaatccc
     5581 cctcacctcc ttggccgcat cacccacgga acccagcaga ccgttcaaca gccctttacc
     5641 gcggcccgga ccgacgtact gatccagtaa ggacaaattc atgcggatat gggccagcga
     5701 catgcccagg ccgtcgtgca ggtccctggc gatccggatc cgttcttcct cctggcccaa
     5761 gaggatgttc tcgactgctt ttcttttcaa ccgtgtgatc tccctgagat ggaacaattc
     5821 tttccgatac aactggtaat accggtaaac gagaatggca ctgatacctg cgatttcaac
     5881 caggaaaaga aatgccagca ggttggagta atagggatag gcgggtatcc agccgaagtg
     5941 caggcaaacc aggaatgcga gagggaccag caaggcggtc gtaatgaaca ggagccagaa
     6001 ctcctctgat tttttattgg ttcgccatgc ccggtacaga aggatagcca ggcatacggc
     6061 agccgccgga agcaacaagg cggtcaattg aacaaagcgc atcattaaag catcgggtat
     6121 gactcccagg gtcgaataaa tcagccggca taccaggttc aaggcggaca atccgatgca
     6181 aattttcaaa aagccaatga gggtggatga ccggtgatct ttaccaatga atttaaggat
     6241 gaaaaggata taaaaaatat ttgcaaggtg cttgcttagt tcgagggcat tgatctgaat
     6301 ccagggctgg ttgggccaga tcattgccgg agcaatgcct ttcacagtgg caatgtatag
     6361 gcataggctg gcggcataga ggaaataaaa tacgatgagc cgggtccgga taatgcatga
     6421 agcaataaaa aggattaaaa tataaaggaa tgcggcgcca aaaagcaggt tccaggtatt
     6481 aaaattcccg gcaggatcat agtcgccccg gaggatgctc aggggcgcat aaagttcggc
     6541 accttttttc tccagcctta aataaatggt gcaggattga ccggccggca attcgacggg
     6601 gaacaggtat tccgtatacc ggaccggctt ggtcgatgac agtaaggcgt tacccgtatg
     6661 cacctgctcc gcttggccgt cccgtacgag ccagaaatcg accgaatgaa tatgcccgtt
     6721 ggccaggcgc agatagaatt tttcttcccg ttcttccggg ttatggagat ggaaacggag
     6781 ccaccaggcg gaaagggtga agccaaaatt ctgggttacc gatccggccc ggaatgccga
     6841 aggtgggaga tggccgacat cctgaaattc caggctgccg ttccggtctt cataaacttc
     6901 aaggctcaaa gccagttgtc ggttggccca atcgagggaa ttcgaggcac aaaccctcag
     6961 gttgcccagg atcaggacca ggcccgggaa tagggctcga aacaaaaagc gaaaggccat
     7021 tttgcgtaaa tcggttttcc aaattaattc cattcatccc ttcctccaaa gatccgggaa
     7081 ggattttact aaaaataccc tccagagggg aggggccggg ccattgccag ccgggtgaat
     7141 aaaaataccc tgtggagggg attgccggtg aggtttttgc cggctaaatt tgaaatggaa
     7201 gcattcatct ggtaaatcaa aacgcatatg aagatgaagt accgtttcag aattaaatgg
     7261 agtaaggtct tgttcgccct cctcagtttt ggcagggcat ggttgcctgc gcaaaccatt
     7321 gatagttata tcggaactta ttccggcgaa aataaaaacg ggtacctgcg gcctgtcgcc
     7381 tcggtgattt cttccgcctt taataccggt caggtgaccc acaccaaact ggacagtaat
     7441 ttcagggttt attttcatct cattggcacc gcttctttta ttcttggcga cggattaaaa
     7501 tattttgacg ggaccactcc tgagggattt caaccctcga aaacgatttc agcccctact
     7561 cttttgggac ccagggagtc cgtgacggtg gagggcatta acgggacggc gtataccttt
     7621 cctgccggca tgggtattcg tatgctgagc ctggtggttc cccagctatc cctggcttat
     7681 ggcaagaccg agctgaattt ccgctttgcc tggggggata ccgatgatga gatcgggagg
     7741 atcaactact ggggattggg attgcgtcat gatcttgggc agtatctgat gccggacgct
     7801 ccctggctgc tatcggtggg cggaatgtat caaaaaataa aggttggtac ttacgcaaat
     7861 ctttcagcgt ataacgcctc tcttacggcc ggtcaggaat ggaggcatgt ttattatttt
     7921 ttccaggcgg gttatcaggc cgggaacctg aaaagccagt acaactattt taacggggag
     7981 aacaaggaag tagtcaaggt ggatctgtcc agtgacaatc cattgttcat cggggccggt
     8041 ctgggcctga aattgggttt tcttcaattg caatggcagg ttcagggata tgaacctgtc
     8101 attggttcct ttgcactcgg cttccgtttt taatccttaa atgaattacc atgaaatctc
     8161 ttttttttct tagcctgctc acggttgcct ggtcctcttg caactacatc accctgagaa
     8221 gacacctcaa tgtgaatgta gacgatgtaa aaacctatga tcaatcaggg gcttttgaaa
     8281 aatcctattt catcaatacg gaaaaaatta taaatgcggt gactgaaaac gtgaataccg
     8341 aagggggcag cattgaaaaa ctggagattg aaagcattca aattacggtc gggttattgc
     8401 caaataacac agcaagttcc gtccacaacg tccatgtgcg cctgggcaat ggatttgtcc
     8461 aggcaggcga atcattggta aaattggata cttcccggtt acccatcaac agtgcgacgg
     8521 tctttctggt caacaatttt atgattttta aaggggtcag cattttgaag acggaattgg
     8581 tcaaaaaact ggctacaggc caatttgaat tttttgaact aaaactggca ggaactattc
     8641 ctgccggtca ggtattccgg ggaagcatga aactgattat taaagcatcc atggatgtgg
     8701 tgacctgcga acgagtcccc ataggattgg gccccagcga atgcatgatt aagaccatgg
     8761 tcctgccctt cgattaacca cgattgcctc ttcattcaaa acaaatatcc ttcatcgcat
     8821 caacatcaac cgccgggtca gggtttgttt tccggcgttt aaacggacca tataggttcc
     8881 aggggccaat tggaaattat cgggccagat gtattcattc cagccggagg gtctggtgtc
     8941 ccggaccagg ccggcaatcc ttctgcccaa tgggtcatac acttccagtc gaacctccac
     9001 gggccgagga agaaaataac ggaaaaaggt tggccctata cccggattgg gataaagggc
     9061 cagttgaatt tgttcttcct cctccggttc agtaccatag ggaacggatg attcagccct
     9121 gagggtaatg accccaaagg atttggtgcc gtttttacag gactgcgggt tgacataata
     9181 aaagacctgc gcttcatttt tattggccgt tgttcttttg aacacctggc gatgtttcag
     9241 ggggaatgga tcctccactt tcgtgcccca gaacgatttc accctttggt gtaagaggga
     9301 atcccactgc ctcagcgtcc gtccggtccc cttgggatca agcaccggcc ctgccgtttc
     9361 agcttcccag attctttctt cataaaattc aagatatatt ccgtcgctgt tgtcccaggc
     9421 attccggagg gtggattcca tggtttcagg gctgttgacg gttcttgcat tttgggattg
     9481 ccatcccgtg atctggccgg cttctccttc acgcagggcg taaggattgg gacagcgggt
     9541 ggcggcctgg gcaaagggcc tgactgcagg gtgtttgcct tcattcgggc agggggccaa
     9601 gggttggttc ccatcctggg gtcttggccc caggccattg tgggagacga tgaatttcag
     9661 tccatgctgg gtgcggccaa gcgccatgat cttctccgtc tgttcggtac cgtcaggcaa
     9721 aggctcggta aacggggcgg tatattcgcc cttattgttg accaggggaa agccggcctg
     9781 gataagggcg tattgcatgt atttatccgg aaacagcgtg tccagcaaat tgaattgctg
     9841 ggtataaaaa tcgtaaaggg cattgggggt gtacccgccc ttcgcccata cctcgggatt
     9901 acagacctcg caaccatcct tgcagttttt gggcagccgg ttctcatgcg taaacaaatt
     9961 cattccggaa ggttttacat atcccagggc ccggtaccag gcgttctttt ctttgatatg
    10021 agcggcaacg tcgcgccaga gatcaaaata ccatttacgg taatggacat ccgaagggga
    10081 tcccatgtcg ccgggtgtac cgcaggtctc ttcatcctca ttgtccctca ggcgcagttt
    10141 ttttaccgtg aagtttccgg cgaccgaggg attaaatatc caggccggtg taccggtcag
    10201 gcctgccttg aatcccaggc tgtacatttt gccattttca accgctttgg ttatttcccg
    10261 gtcgagaaag gtccagtcgt acttgcccgg tccgagatgc acttcatccc agcgcagccg
    10321 tatgaacacc ccggtaatat tggggttttt ccaggtccaa tccatggttg cataatccgg
    10381 gccgcgctcg cagagaaatt gatttcccgc cgcggctttc aacaggttct ggggacagtt
    10441 ttgccccgtg gaaaggatct caaagccggg gccatccggg cttagggcca cgatgcctgg
    10501 catgagcgaa gccgcgggtc cggtaaaatc gttggggtcc gcaaacaccc ttggcttgaa
    10561 ttggtcgcat tgaagggaaa ggctgtcagc cagggtgatt ggcgctacgg attgattccg
    10621 gttgctgaac aaccattcca cggtatccag cgcgttaagg tgtatatacc tcggcgtaaa
    10681 ggatccatcc ggatcaacac gaacctggtg taatgcgggc ttttgggccc ggagttccgg
    10741 gcaggttaag gttgaggtta aggttaaggt taaggttgag gttgaggtta agaaagcgag
    10801 gattgttttc atttcagctt ttgaattggt tggaatggaa tgattccaaa aggtttaatt
    10861 cctgtcgtca ttttggatta gcgataagcc caaaccgcta ccctggaccg gcccttgcga
    10921 tatcctaccg tctcatacca gaaagtgatt ttctgcaaat gccttaatcc gccttccagg
    10981 tcaatgaccc tgctttgctg accctgacgg atgaccgatc gaagaggcac gtcctggacc
    11041 gccccgttgt caaaatggac tttcatgtcc atcacatgaa gcgggccatc cgtgaccctg
    11101 agccggatct gccggaagtc atcttttata tttcccaaaa acagttcgtc atgatctgcc
    11161 ccgaaattca cccatttgtc cccgagaaat ctccacccgc catccgcgat agccgcgacc
    11221 gctaccggtt tgggagaaca ggagatgaaa aacaaaaaca agccgccaat aaaaagaagg
    11281 agatttttca taatacaggg tttaggattc cagcaaaatt aacctctttg tatagtttaa
    11341 acactacccc taggggtgaa ttgaagtgag aaacgagcag cgagaaacga gcagcgggaa
    11401 acggatccac taccctcaat tcaattcgca gatctgctgg atccgggcat ggtccaggat
    11461 acggatgccc ctgccatgga gttcgatcag ggattcttct gaaaacgagg acagggtgcg
    11521 gatcacattg gccgtagtca ggttggtcag ggcggccagg tcggagcgtt tgagggtgga
    11581 atcaataaaa tgatcgacgg gccgttcacc gaaatattct cgaaggtaga gcaaggcata
    11641 agccaggcgg ccacgcatat gtttttgagt gagttgaagc aagtgatttt ggctttcttc
    11701 aagcaagcgg gcaagataaa tgctcacccg gctggcaaac aggctgttgt tcctgaagac
    11761 ttgcataaaa tcttcctttt cgacgatgca gatttcagcg tcttccaggg ccatgaccga
    11821 tgtttggtgg ttggaatgga gcagcagatc aggcaatccg acaaattcca ccggtttctt
    11881 caaggagagg atcagtttat ttcccgaagg tgccgtcttg aattctttca atttgccgct
    11941 ttttaaacaa atcaatccct ggattttgtt tccttcttta tacaggatct ccccggcccg
    12001 gtaggtgaca atcgatttat ggcttttcag gatctcaaga tatttggtct ccagcccatc
    12061 aaaaagtgaa aatttccgga acgggcaggt cacacaattc ccggattggg agatggcgat
    12121 ggtatataaa ggcaagacgg gattcaaagg cataaaacag tatgatttta tcttattctg
    12181 gtaccaagat agatcataat gttgagccta tggaccggac aggccgggat gcggacattc
    12241 taattaaatt cttatgtccg gaaaaaacca ggagcacacc cactgttggg catgcccctt
    12301 gctatctatt tcaatttgaa cattaatttc tactgaacct tccagggcag ttttaatatt
    12361 ctcctggccg tttgctggcc caaagtcaaa cctgcatcac aatccatccg gaagtgcaca
    12421 cccagggtga ttcgcgacaa ggcattttcc ttcatagcgt cttcaaaaga attgaaagtg
    12481 cgtggagtgc ctaaaaattc gatccgtcct ttatgacaga ggtctgtaaa ttcataattt
    12541 gacccaaaca gatgcgcgag gatgacgcct gaaccaccgg caaaggtcga atgaccagaa
    12601 ggataagccg gaaatggtgg ggtgagaccc tggtctccgg aaagtgcatt ggtcaggata
    12661 ggttcccatt tggggtcaaa cagcttctgg atataagtct ccgggcgaac tacattgtat
    12721 acccacttgg aatgccagca ggccacagcg aaatcattca aagccaggcc gattttggct
    12781 accgattcca cagctttctc caggtttatt ttttcagaaa ccatgatttc attcaaaatg
    12841 gcaataggcc tggagggagg actgaaagtt tggccttcga tgtcatcact ccagaattca
    12901 gagatccacc ggtcttcata aggcatgccg gcattggata aggtaaatac ttccagggcc
    12961 tgggcataaa aggctgaagt cgggttttca ctataagcaa gtggcggcgg acagagcttt
    13021 tgggcctccg aggttgcaaa agttctgaat cttcctccga atgggaatct tcccccgt
//
LOCUS       DAOQXT010000121        12986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5304_length_12986_cov_5.998685, whole genome shotgun sequence.
ACCESSION   DAOQXT010000121 DAOQXT010000000
VERSION     DAOQXT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5304_length_12986_cov_5.998685"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1397)
                     /locus_tag="PKM27_16540"
     CDS             complement(177..1397)
                     /locus_tag="PKM27_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNR08929.1"
                     /translation="MKSKNTLIAVGILVSCWVFIAWKPNWGFFAHKRINRLAVFTLVP
                     DMMPLFKTNIEFITAHAVDADKRRYAVSDEAIRHYIDLDLWPRVPQDYLDALILYGDL
                     VVESPGGNLQSIFPEERIVRDQKKQWIRIGADSGKRVDWHAFRRFLKEELKVDQRGQD
                     LPALTTDSFNHYFGQTLLAGGQSLFWIDTFSRHGILPYHIQAMYQRLVNAFKTGEAPV
                     ILRLATDLGHYVADAHVPLHTTSNYNGQQTGQTGLHAFWESRIPELFADESYDYLVGK
                     ARYIRDVGKFSWDMIHQSHQLVDSVLGIEKRLSRTFPPDEQYCFDQRAGLTVRTQCPA
                     YAGAYQSAMKGMVENRMRDAILALGSLWYSAWVDAGQPVLSGRTDPVSDIHPVDSTLE
                     KAYRDKKILGRPED"
     gene            complement(1407..2333)
                     /locus_tag="PKM27_16545"
     CDS             complement(1407..2333)
                     /locus_tag="PKM27_16545"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNR08930.1"
                     /translation="MRINARLVILLIWLVGYGCKTDPARQETDFRISDTAAVHRIEIY
                     APDGEKQNLERRPDGWWINGKFPVRPDAIANIFRILPGVQVMFYPPDAAIAQMMQAIR
                     GHGVRLVFLDRGGDPIKSYRIGGMTNDERGTYALMDGFERPYVVHVPGFEGSLANRFT
                     MPVEDWRDRMLFRHRPESLTGLKLSYPDHPEEGFEIKRMEGRFELYDYGGRLMQTGES
                     GIRAYLDVFSGIGVESIENDFEGIPEVLASRPHAVLDLNFLPDSSRTLKFFPIYVEGQ
                     QEVERLFVYDGQDFFLAQTRILQKIFRSSAAF"
     gene            complement(2323..4746)
                     /gene="gldG"
                     /locus_tag="PKM27_16550"
     CDS             complement(2323..4746)
                     /gene="gldG"
                     /locus_tag="PKM27_16550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HNR08931.1"
                     /translation="MNAIIRKELQSYLSSWSLMLAMAAYFLLVGLWLWLFPGTSIPDN
                     KFATLQTLFDLAPWMFLFILPAMTMKSIAEEKITGTLDLLKSKPISVSSLILGKYFAI
                     LIVVAVLLLISQVYTWSVYQLGFPKGNLDLGGTLGSFIGWFLLAAAYTSIGILSSAMA
                     PNQMTAFLLAAGLSFLMFTGPSFLSTLPLFEGTLDYMIQWIGIQYHYQSLSRGVLTSQ
                     DLFYFIALNVLFLQLAVFAVQANGQTGLGSNRFQKWLLLPVGILLMSGLKLFSLDLTG
                     DKRHTITKESKELVASVRDNIYVKILFTGKFPAAFTRLKKTAVETLSSFNRINKKILF
                     EEVDPLQGDPELVRSNQESLGRDGIVPTRLTVYNGREQEQKIVYPYALFYFGERMIPV
                     SLLESMDPNISEDEVLNRSASLLEYKFANAIQKLQMSKNPVVVFTRGHGELPQINTAD
                     LERSLRPVYSTGRLTLDSVVQISPEIDVLVVAKPQRAFSDRDNFLIDQYIMNGGKVLW
                     FIDPLLVNTDTVNAANRSKLDFIPLPYDLNLEELLFKYGCRIQPNIVLDYECSTIPLL
                     AGYSNNTPQFEPRFWYYHPLVVPASNHPVVKNLDRISLFYPATVDTVRTKTAVKKTVL
                     LASSAYSRTQMSPSPVNFEMIRSLEKPEQFNRESQPLGLLLEGVFPSAFENRLTGPFQ
                     ATLSQIGQSFKTESKPTRMIVYSDGDLVANAVSSRGEPAPLGFNIYEQKTYPSNKNLV
                     LNSIEYLIDEHQVMQARNKELRLRMLDSARLSREADLWRWFNILFPAALVFLLAAVFN
                     YWRKKQYAH"
     gene            complement(4753..5679)
                     /locus_tag="PKM27_16555"
     CDS             complement(4753..5679)
                     /locus_tag="PKM27_16555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR08932.1"
                     /translation="MEILEADQLVKQYGPQTAVDQVSFRIQSGEIVGFLGPNGAGKST
                     TMKMISGILEPDEGSVKIKGSVLDKNHPSLKQSIAYLPENNPLYTDLYVRESLEFMAR
                     IHGVKNAKPVIDEAIRACGLSQEKHKKIGQLSKGYRQRVGLAQTLLHQPDLFILDEAT
                     TGLDPNQILEIRDLIRSLGKEHAVLMSTHILQEVESICTRCIVIHRGKLIADEPMVSL
                     KKKLQARHEVYVRFVGEVPVALLRKRWNQIVPGTEEQSFLIRDEDPLLSQKVYQWAVE
                     NHLVLNELKWVENKMEDLFHSLTGQTSGAAHA"
     gene            complement(5686..6453)
                     /gene="truB"
                     /locus_tag="PKM27_16560"
     CDS             complement(5686..6453)
                     /gene="truB"
                     /locus_tag="PKM27_16560"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNR08933.1"
                     /translation="MDLLNIKPVRLDDEFSDPAYWEEGRLLLIDKPLTWTSFDLVNKC
                     KLALRSGLNIRKIKIGHAGTLDPLATGLMLILTGKFTRLTPMIHEMDKTYEATLFLGA
                     TTPCYDAERPVDALYPTGHITPELIRETANRFTGRLVQHPPVFSAVKINGKKAYKSAH
                     RGREVKTRPRFIDVHRLDLEHIDLPYVSFEVTCSAGTYIRSLAHDMGKAMDSGAYLFS
                     LKRTRIGPWSLDEAMDIRAFSEKLNSAPASQPLCPNP"
     gene            complement(6544..6915)
                     /locus_tag="PKM27_16565"
     CDS             complement(6544..6915)
                     /locus_tag="PKM27_16565"
                     /inference="COORDINATES: protein motif:HMM:NF022741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNR08934.1"
                     /translation="MSKQTNPAKKIIKTETKKPEVFSGPSTPIAARRKTTTSLTNTPV
                     EFTFNKDNIRWLLISVGLVILGYALMMGGRMPSNEVWDEKLIYSFRRTVISPIVILGG
                     LVLAIYAIFRNKPDTKPSAES"
     gene            7129..7758
                     /locus_tag="PKM27_16570"
     CDS             7129..7758
                     /locus_tag="PKM27_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08935.1"
                     /translation="MHANRMLFRLRMGMVFILFCSYLYGQQQGVFNPGFEGEPQDATM
                     PIGWMGCEDGTTPDILPGPWGVFQPAYEGQTYMGLIVRKNGTWECVGQRLSESLKAGN
                     CYFFKLMLANAKTYNGYNKAAVLRVWGGDDRCEKKQKLFESAPVSHADWKPYTVRFKP
                     KQNISFLIIEAFYNTKQQHTYNGNILIDAMTSIQPCDRAELIVKSKPAF"
     gene            7879..9192
                     /gene="ahcY"
                     /locus_tag="PKM27_16575"
     CDS             7879..9192
                     /gene="ahcY"
                     /locus_tag="PKM27_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNR08936.1"
                     /translation="METVHTRLPYKVKDISLAEWGRKEIKLAEAEMPGLMALRQQYGL
                     SKPLKGARVAGCLHMTIQTAVLIETLAELGAEVSWSSCNIFSTQDHAAAAIAAEGIPV
                     YAWKGMSIEEYDWCIEQTLYAFKGGKPLNMILDDGGDLTNIVLDQFPELVKELTGISE
                     ETTTGVHRLYERVAKGTLPVPAINVNDSVTKSKFDNKYGCRESCVDAIRRATDLMLAG
                     KVAVVAGYGDVGKGSAASLRGAGCRVIITEIDPICALQAAMDGFEVKKMKNAIQEADI
                     VVTATGCLNVVTGEHFKMLKDKAVVCNIGHFDNEIDVAWLNKNYGNTRDQIKPQVDKY
                     TIEGKDVILLAEGRLVNLGCAMGHPSFVMSNSFTNQVLAQIELWTNKGKYENKVYMLP
                     KHLDEMVARLHLEKIGVELDELDHEQASYLGISQEGPYKPDYYRY"
     gene            9262..9849
                     /locus_tag="PKM27_16580"
     CDS             9262..9849
                     /locus_tag="PKM27_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNR08937.1"
                     /translation="MNSEFYTYFQLGLQHIADLNAYDHILFIVALCAIYRPEDWKKVL
                     ILVTAFTIGHSLTLAMAALDLIRFRQELIEFLIPVTILFTAALNLLPGAEKSRQHMPL
                     WKYLLPLVFGFIHGMGFSNYFKSLLGKEESVVKPLFAFNLGIEAGQIGIVVALMILSF
                     LVMGLARIKQPVWTRLVSGIAAVMALFLMWETKFW"
     gene            9870..10388
                     /locus_tag="PKM27_16585"
     CDS             9870..10388
                     /locus_tag="PKM27_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08938.1"
                     /translation="MIEGFLLNFILAMCYPTLVAGADLHDIHVSKCEIQLNEESSSLE
                     VATQIYLDDLQSILSKHGADNLHLCTERESKDADTFLKAYLEKKLQISADGKLLSHQF
                     VGKEPSEDQIAVWCYIELKVPPGFASLKVKYDVLMDLYDDQKNIVNIKARGKNGFMLF
                     STRHFEETIQYK"
     gene            10404..10667
                     /gene="yidD"
                     /locus_tag="PKM27_16590"
     CDS             10404..10667
                     /gene="yidD"
                     /locus_tag="PKM27_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNR08939.1"
                     /translation="MSDKIISFFTFLLKQLVILPIRLYQVLLSPLLGANKCRFQPTCS
                     HYMIGAIQEWGVLKGLWLGLKRISRCHPWGGFGEDPVPKKEKK"
     gene            10752..11282
                     /locus_tag="PKM27_16595"
     CDS             10752..11282
                     /locus_tag="PKM27_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08940.1"
                     /translation="MSALLNIQIQPKPEPHATLSGRFSPILQSRMQAFRGKKISGNEF
                     ESLYALPSVLEAVLVSIQLQQAIRTEGLAEAKIGLHADLSLNADQPVRPLDLEILDRI
                     LELGAPNTVVMSEPVAEQINNHPEFRTGKIGKIHHKAYAGGLNIYSLHGYGLSSLPAS
                     RFYERLKARLKIASLF"
     gene            11434..12327
                     /locus_tag="PKM27_16600"
     CDS             11434..12327
                     /locus_tag="PKM27_16600"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNR08941.1"
                     /translation="MSSTEKTVLTIVAGIQAPVEKVWACWTEPRHIIKWNAASPDWQC
                     PYAENDLRPGGKFMSRMEAKDGSYGFDFAGEYTRVDPLRLIEYTLGDGRKVQTTFVSS
                     GGETRIISHFEAENSNSLEMQQQGWQAILNNFKKHAESAGTETMQFEVKIQAPVEKVY
                     SNMLDDIQYRAWTSVFNPTSHYKGSWEKGSKILFIGTDQDGNTGGMVSRIRENIPNTF
                     VSIEHLGVVQGEQEITSGPQVEAWAGALENYTFRRDGTGTRLVVDLEANEEYKSYFEE
                     HWPKALDKLKSICEGYPASAN"
     gene            12365..12808
                     /locus_tag="PKM27_16605"
     CDS             12365..12808
                     /locus_tag="PKM27_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR08942.1"
                     /translation="MASVSTYLNFQRETEAAFNFYKSVFGGEFIGAVSRFRDIPPTEG
                     MPELPEADKDLIMNIALKILGGHLLMGTDAPESMGFKLRQGNNVYITLHPDTRAETKK
                     LFDALSDGGVVEQELQDMFWGDYYGSCRDKFGVQWMFNCSEKDNR"
     gene            complement(12739..>12986)
                     /locus_tag="PKM27_16610"
     CDS             complement(12739..>12986)
                     /locus_tag="PKM27_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08943.1"
                     /translation="DRLDREVVRSDPNGFRIFTKYDILGRVILTGKDTTMANPSGGEH
                     PDGVQEKMNTTALRNQRLSFSEQLNIHCTPNLSLQLP"
BASE COUNT         3200 a         3366 c         3379 g         3041 t
ORIGIN      
        1 gtaatatgtt ttaaaatacc atattacaag tctaaaggaa gcttagcttc ggcctccata
       61 tctcccacga agggcacaaa gccaccaggt tcggatgaag cttggcttcg gctcatcaga
      121 cattttgtgt tattgatctg aataaatcaa tctccatcgg cgagcctacc cttaactcaa
      181 tcctccggcc tccccaatat cttcttatcc cggtaggcct tctcaagggt tgaatctacg
      241 ggatggatat ccgatacagg atccgtcctt cccgatagca caggctgacc cgcatccacc
      301 caggcggaat accataaact gcccagtgcc aggatggcat ccctcatcct gttttcaacc
      361 atgcctttca tggcggactg gtaggctccc gcataggccg ggcattgggt gcgaacggtg
      421 aggcccgccc gctgatcaaa acaatattgt tcgtccgggg gaaaggtccg gctgagcctt
      481 ttttcaatgc ccagaacgga atccaccagt tgatggcttt gatggatcat gtcccaggag
      541 aatttgccga cgtcgcggat atacctggcc ttccctacca ggtaatcata ggattcatcg
      601 gcaaacaatt ccgggatccg gctttcccaa aaggcgtgca aacccgtctg gccggtttgc
      661 tggccgttgt aattactcgt ggtatgtagg gggacatggg catcggcgac atagtgtccc
      721 agatcggtgg ccagccggag aatgaccggg gcttccccgg ttttaaaagc attcaccagc
      781 ctctgataca ttgcctggat atggtaaggc aggatgccat gccggctgaa agtatcgatc
      841 caaaacaacg attgtccccc ggccagcaag gtttgaccaa aataatgatt gaagctatca
      901 gtagtaaggg caggaaggtc ctgacctctt tgatccacct tcagctcctc tttcaggaac
      961 cggcggaagg catgccaatc tacccgcttg ccggaatcgg cgccgatccg gatccattgt
     1021 tttttttgat cgcggacgat tctttcctcc ggaaatatag attgaagatt tccaccgggt
     1081 gattcaacca ccaggtcacc ataaagaatc agggcatcga gatagtcctg gggcacccgg
     1141 ggccaaaggt ccagatcgat ataatgccgg atggcttcat cggaaaccgc ataccgtctt
     1201 ttatccgcat ccacggcatg ggcggtgatg aattcaatat tcgttttgaa gaggggcatc
     1261 atgtccggga ccagcgtaaa gacggcgagc cggttgatgc gcttgtgggc aaaaaaaccc
     1321 caattgggct tccaggctat aaaaacccaa caggatacca atatgccaac ggcaatcagg
     1381 gtgttcttcg atttcatctg actttattaa aaagccgcgg atgacctgaa tattttttgt
     1441 aagatacggg tttgagcaag aaaaaaatcc tgcccgtcat aaacgaataa gcgctccacc
     1501 tcctgctggc cctctacata aatgggaaaa aatttgaggg tcctggatga atccggcagg
     1561 aagttcaagt cgagcacggc atgcggccgg gaagccagca cttcgggaat gccttcaaaa
     1621 tcgttttcga tgctctccac tccaataccg gagaacacat cgaggtaagc acggatcccg
     1681 gattcaccag tttgcatcaa acggccgccg taatcataca attcaaaccg gccttccatt
     1741 cttttaattt caaatccctc ttcgggatgg tccggataac ttaattttaa accggtcagg
     1801 ctttccggcc ggtgcctgaa cagcatccgg tccctccagt cttcgaccgg catagtaaac
     1861 cggttggcga ggctgccttc aaacccgggc acatgaacca cataaggccg ctcaaagcca
     1921 tccatcaagg cataagtgcc cctttcatca ttggtcatgc cgccgatgcg gtaagatttg
     1981 atcggatccc ctccacggtc caggaaaacc agcctgaccc catgcccgcg aatcgcctgc
     2041 atcatttgcg cgatggccgc atccggaggg taaaacatca cctgcacacc cgggaggatg
     2101 cggaaaatat tggcgatggc atcgggacgc acgggaaatt ttccgttgat ccaccaacca
     2161 tccggtctgc gttcaaggtt ttgtttttca ccatccggcg cataaatttc aatacggtgg
     2221 acggcggcgg tatctgaaat ccggaaatcg gtttcctgcc gggcagggtc cgtcttgcat
     2281 ccataaccca ccagccaaat cagcaggatc accagtctgg cattaatgcg catattgctt
     2341 ttttctccag taattaaata ccgcggccag gagaaaaacg agtgctgccg ggaagaggat
     2401 attgaaccat ctccagagat cggcctcccg gctcagcctg gctgagtcga gcatgcgcag
     2461 ccggagttcc ttattcctgg cctgcatgac ctggtgctcg tcgatgaggt actcgatgct
     2521 gttcaggacg aggttcttgt tggaaggata ggttttttgt tcgtagatat taaaaccgag
     2581 cggcgcgggt tcgccgcggc tgctgaccgc gttggcgacc aggtcaccgt cgctgtacac
     2641 gatcatgcgg gtaggtttac tttcggtctt gaagctttgg cctatctggc ttaaagtggc
     2701 ctgaaaaggc ccggttaacc ggttttcaaa ggcggatggg aatacccctt ccagcaaaag
     2761 gcccagaggc tgactctccc ggttgaactg ttccggtttt tccagactgc ggatcatttc
     2821 aaaattgacc ggcgaagggc tcatttgggt ccggctgtat gcactgctgg ccagcaggac
     2881 ggtctttttc accgctgttt tggtgcgcac ggtatccacg gtagccggat aaaaaaggct
     2941 gatgcggtcc aggtttttca cgaccggatg attcgaagcc ggcacgacca ggggatggta
     3001 ataccaaaac cggggttcaa actgcggtgt attgttggaa taacccgcca acaagggaat
     3061 ggtgctgcat tcataatcca gcacgatatt gggctggatc cggcatccgt atttaaacaa
     3121 cagctcctcc aggttcaggt cgtaaggcaa aggaataaaa tccagcttac tgcggttggc
     3181 ggcatttacg gtatccgtat tgaccagcag ggggtcaata aaccacaaca ccttaccccc
     3241 gttcatgata tattgatcga tcaggaaatt gtcgcggtcg ctgaatgccc tttgcggctt
     3301 agcaaccacc aggacatcta tttccgggga gatctgcacc acgctgtcga gggtcagccg
     3361 gccggtcgaa tacaccgggc gcagggaccg ttccaggtcg gcggtattaa tctgtggaag
     3421 ttccccatga ccccgggtaa ataccaccac gggattttta ctcatctgga gcttctggat
     3481 cgcgttggca aatttatatt ccagcaggct ggcagaccgg ttgagcacct cgtcttcaga
     3541 aatattgggg tccatgcttt ccaggaggct gaccgggatc atgcgctccc cgaaataaaa
     3601 aagggcatag ggatacacga ttttttgttc ctgctcccgg ccgttataaa cggtgagacg
     3661 cgtcgggacg atgccatccc gacccaggga ttcctggttg gaccgcacca gttcggggtc
     3721 gccctggagc gggtccactt cctcaaacaa gatcttttta ttgatccggt tgaaagaaga
     3781 caaggtttca acggccgtct tcttcaaccg ggtaaaggcg gccgggaact taccggtaaa
     3841 taagattttg acatagatat tgtcgcggac actggccacc agttccttcg attctttcgt
     3901 gatggtatgc ctcttgtccc cggtaaggtc cagcgaaaac agtttcagcc ccgacatcaa
     3961 taatataccc accggcaata agagccactt ttggaaccgg tttgagccaa gcccggtctg
     4021 accgttagcc tgaacggcga aaaccgccag ttgtaaaaac aatacgttga gggctatgaa
     4081 ataaaagaga tcctggctgg taagcactcc ccggctcagg gactggtagt ggtattgtat
     4141 gcctatccac tgaatcatgt agtccagggt gccttcaaac aaaggaaggg tgctgaggaa
     4201 ggacggtccg gtaaacatca aaaaactcag gccggcagcc agcaaaaacg cggtcatctg
     4261 gttgggagcc attgcggaag acaagatccc gatggaggta taggccgcag ccaataaaaa
     4321 ccaaccgatg aaggaaccca gggtgccgcc aaggtccagg ttgcctttgg gaaagcccaa
     4381 ttggtacacc gaccaggtgt agacctggct gatcagcagg aggaccgcca ccacgatcag
     4441 gatggcaaaa tatttaccca ggatcaaaga tgaaacagaa atgggtttgc ttttaagcag
     4501 gtccagggtg cccgtgattt tctcttcggc gatggacttc atggtcatgg ccggcaggat
     4561 aaaaagaaac atccagggcg ccaggtcaaa caaagtctgc agggtggcga acttattgtc
     4621 cggaatgctg gtacccggaa acagccacaa ccaaagcccc accagcaaaa aataggcagc
     4681 catggccagc atcaggctcc aggagctcag gtaggattgc agttctttcc ggatgatggc
     4741 attcatgggt gactaagcat gggctgcccc ggaggtttgg ccggttaatg aatgaaaaag
     4801 gtcctccatt ttgttttcca cccatttgag ttcattcaaa accagatggt tttccaccgc
     4861 ccattgatac actttctgac tgagcaaagg gtcttcatcc cggatcagga aggattgttc
     4921 ttccgttcct gggacgatct gattccagcg tttccgtaat aaagcgaccg gcacttcgcc
     4981 gacaaaacgc acataaactt catgcctggc ctgcaacttt tttttcagtg aaaccatggg
     5041 ttcatcggcg atcagttttc cccggtggat gacgatacag cgggtacaga tggattccac
     5101 ctcctgcagg atatgcgtag acatgagcac cgcgtgttcc tttcccaggg accggatcag
     5161 gtcccggatc tccaggatct ggttggggtc gaggccggtg gtcgcttcat ccaggataaa
     5221 aagatcgggc tggtgcagga gcgtttgggc gaggcctacg cgctggcggt aacccttgct
     5281 cagttgtccg atttttttat gtttttcctg gctgaggccg caggcccgga tggcctcgtc
     5341 aatcacaggt tttgcatttt ttaccccgtg gatcctggcc atgaattcca gggattccct
     5401 tacataaagg tcggtataca aggggttgtt ttccggcaga taggcgatgg actgttttaa
     5461 ggacggatgg tttttatcca gcacactgcc cttgatcttt acggaccctt catccggttc
     5521 cagaatgccg gagatcattt tcatcgtcgt ggatttaccc gccccgtttg gacccagaaa
     5581 gccgacgatc tccccgctct ggatccggaa agagacctgg tccaccgcgg tctgcgggcc
     5641 atattgtttt acgagctgat cagcttccag gatctccatg gggcattaag gattggggca
     5701 taagggttga gaggctggcg ccgagttgag tttttcggag aaggcccgga tatccatggc
     5761 ttcatccagg gaccagggcc cgatgcgggt ccgtttcaaa gagaaaagat aagcgccgga
     5821 gtccatggct ttacccatat cgtgggccag gctccggata taggttccgg cgctgcaggt
     5881 tacctcaaaa cttacatagg gcaggtcgat gtgttcaagg tccagccggt gaacatcgat
     5941 gaacctgggc ctggttttta cctccctgcc ccggtgcgcg gatttataag cttttttccc
     6001 gttgattttc accgctgaaa ataccggggg atgttgcacc aggcgcccgg taaaacgatt
     6061 ggcggtctcc cggatcaatt ccggagtgat atggccggtt ggataaaggg catcgaccgg
     6121 cctttcggca tcgtaacagg gggtggtggc gccaagaaac aaggtcgctt cataggtttt
     6181 gtccatttca tggatcatgg gggtcaggcg ggtgaattta cccgtcagga tcagcatcaa
     6241 gccggtggcc aggggatcca gggtgccggc gtggcctatt ttgatttttc tgatattcag
     6301 gcccgaccga agggccagtt tgcatttgtt gaccaggtca aaagaagtcc aggtcagggg
     6361 tttgtcgatc agcaggagcc tgccttcctc ccaataagcc ggatccgaaa actcatcgtc
     6421 aagccttacg ggttttatgt ttaacaggtc catgagaagg tataagtacc cgcaaaaata
     6481 gggctttggg agcggccgtg tggaaataga aaaagggccg ggatcttcag ttcatcgccg
     6541 ggatcaggat tccgcggatg gtttggtgtc cggtttgttt ctgaatatgg cataaatggc
     6601 caggaccagt ccgcccagga tcacgatcgg ggagatcacg gtgcgcctga aactgtagat
     6661 cagtttttca tcccagacct cgttgctggg catgcggccg cccatcatga gggcgtatcc
     6721 caggatgacc aggccgacgc tgatcaaaag ccaccggata ttgtctttgt tgaaggtaaa
     6781 ctctaccggc gtattggtga gggaagtcgt ggttttcctc ctggctgcga tgggagtgga
     6841 gggcccggaa aagacttcgg gctttttggt ctccgttttg atgatttttt tcgccgggtt
     6901 ggtttgtttg gacatgctca aattttgccc aaaattaact tttttgctat atataaaacg
     6961 aatttataca gggagggaaa taaaacatag gcacccgacc tattgttgtg ttgcagccgg
     7021 ataccgggca tcttttcgga ttcaggatct tcttgattcc ggatccggga ggacatttcc
     7081 tacaaatacc tcaaatttgg cttgaaattc gcttgtttaa tcttgtttat gcacgctaat
     7141 cggatgctgt tcagactgag gatgggcatg gtctttatcc tcttttgttc ctatttatat
     7201 gggcagcaac aaggagtctt caatccgggt tttgaagggg aaccccagga tgctaccatg
     7261 cccatcggct ggatgggctg tgaggacggc accactccgg atatccttcc cggcccctgg
     7321 ggcgtatttc agccggccta tgaaggacag acctatatgg gcctgatcgt gcgaaagaac
     7381 gggacctggg aatgtgtcgg gcagcgattg tccgaatccc tcaaggcggg caattgttat
     7441 tttttcaaac tgatgctggc caatgcaaaa acttataatg ggtataataa agcggccgtc
     7501 ctccgggtct ggggaggcga cgaccgttgc gagaaaaaac aaaaactttt tgaaagcgcg
     7561 cccgtcagtc atgccgactg gaaaccctat acggtccgct ttaaacccaa gcagaacatc
     7621 agcttcctca tcatcgaagc cttttacaat accaaacaac aacataccta caacggcaat
     7681 atccttatcg atgccatgac ttcgatccag ccctgcgacc gcgccgaact gatcgtaaaa
     7741 tcgaagcccg ctttctgacc ggaagcggcg tgccggtgcc tttgaatggc attgcaccgc
     7801 gatgcattta ttatgccgcc atatccatct atttgatacc tttgcgcccg aattcataac
     7861 atatattaaa aaatccgaat ggaaaccgta cacaccagac tcccctacaa agtcaaggac
     7921 atcagcctgg ccgaatgggg aagaaaagaa atcaaactgg cggaagccga aatgcccggg
     7981 ctcatggccc tgcgccagca atatggcttg tccaaacccc tgaaaggggc cagggtcgcc
     8041 ggatgcctgc atatgaccat ccagaccgcc gtgctcattg aaacgctggc cgaactcggc
     8101 gccgaagtgt cctggtcctc ctgcaatatt ttttctaccc aggaccacgc cgctgcggcc
     8161 attgcggccg agggcatccc ggtctatgcc tggaagggca tgagcatcga agaatacgac
     8221 tggtgcatcg aacaaaccct ttacgccttt aaaggaggca aacccctgaa catgatcctc
     8281 gacgacggag gggatttgac caatatcgtg ctggaccaat ttccggaact ggtgaaggaa
     8341 ttgacgggca tcagtgaaga aaccaccacc ggggtccacc ggctttatga gcgggtcgcc
     8401 aaaggcaccc tgccggtccc ggccatcaat gtcaacgatt ccgtgaccaa gtccaaattt
     8461 gacaataaat acggttgccg cgaaagttgc gtggacgcca tccgcagggc taccgacctg
     8521 atgctggctg gcaaggtggc cgtagtcgcg ggatacggag atgtgggcaa aggttcagcg
     8581 gcttccctgc gcggggccgg atgccgggtg atcatcaccg aaatcgatcc catctgcgcc
     8641 ctccaggccg ccatggacgg tttcgaagtg aaaaaaatga aaaacgccat ccaggaagcg
     8701 gatatcgtgg tgaccgccac cggttgtctc aatgtcgtga ccggagaaca tttcaaaatg
     8761 ctcaaggata aagccgtcgt ctgcaacatc ggtcatttcg acaacgagat cgatgtggcc
     8821 tggctgaata aaaattatgg aaatacgagg gaccagatca aaccccaggt ggataagtac
     8881 accatcgagg ggaaagacgt gatcctgctg gccgaaggcc gcctggtcaa cctgggatgc
     8941 gccatgggcc atccttcctt cgtgatgtcc aattccttta ccaaccaggt tttggcccag
     9001 atcgaactgt ggaccaataa aggcaaatac gaaaacaagg tctatatgtt acccaaacac
     9061 ctggatgaaa tggttgcccg gctccacctg gaaaaaatcg gcgtggaact ggacgaactg
     9121 gaccacgagc aggccagcta cctcgggatc agccaggaag gcccctacaa acccgattat
     9181 taccgctatt aataaggccg ggtatatccg gggtaaaagg acggtgatta taaagaaaga
     9241 tcaggatctt ttgaataagc catgaattct gagttttaca cctattttca actgggcctt
     9301 cagcacatcg cggacctgaa tgcctacgat catatcctct ttatcgttgc cctctgcgcc
     9361 atctaccggc ctgaggactg gaaaaaagta ctcatcctgg tgacggcctt taccatcggg
     9421 cattcgctga ccctggccat ggcggccctg gacctgatcc gtttccgcca ggaactgatc
     9481 gaattcctta ttccggtgac catcctcttc accgctgccc tgaacctcct gcccggcgcg
     9541 gagaaatccc gccaacacat gcccctttgg aaatatctgc tccccctggt cttcggcttc
     9601 atccacggca tgggtttttc caattatttc aaaagcctac tgggcaagga agaaagcgtg
     9661 gtcaaacccc tgtttgcctt caacctgggc atcgaagcgg gccagatcgg catcgtggtc
     9721 gccctgatga tactcagttt tttggtgatg ggtctggcaa gaatcaaaca gccggtatgg
     9781 acccggctgg tttcggggat cgccgccgtg atggcgctgt tcctgatgtg ggaaaccaaa
     9841 ttctggtgaa tttggttata gggaaacaga tgattgaagg cttcctattg aattttatcc
     9901 tggccatgtg ctaccccacc ctggtggcag gggcggattt gcatgatatc cacgtcagca
     9961 aatgcgagat ccagctcaac gaagaaagct cctccctgga agtggccacc cagatctacc
    10021 tcgatgacct gcagtccatt ctttcgaagc acggggcgga caacctccac ctctgcaccg
    10081 agcgggaaag caaagacgcg gatacctttc tgaaggctta tcttgaaaaa aaattgcaga
    10141 tttcagccga tggcaagctg ctttcccatc agtttgtcgg caaagagcct tccgaagacc
    10201 agatcgccgt gtggtgttat atcgaattga aagtccctcc cggttttgcc tcgctcaagg
    10261 tcaaatacga tgtcctcatg gacctgtacg acgaccagaa aaacatcgtg aacatcaagg
    10321 cccggggaaa aaacggattt atgcttttca gtaccaggca ttttgaagaa accatccaat
    10381 ataaataaag cagggcaacc ggtttgagcg ataaaatcat atcttttttc accttccttt
    10441 taaaacaact ggtcattctt cccatacggt tataccaggt tttgttgtcg cccctgctgg
    10501 gagccaataa atgccgcttc caacccacct gctctcatta catgatcggc gcgatacagg
    10561 aatggggggt cctgaaagga ctttggctgg gcctgaaacg gatctccagg tgccatccct
    10621 ggggcggatt cggagaagac ccggtcccta aaaaagaaaa gaaataaacc tcgggcaggt
    10681 aacgaaatcc ccggatgctt acactttaaa taaaggccaa acagcctctt gtattcaaat
    10741 catccgtagc catgtctgcc ctgttaaaca tccagatcca accaaaaccg gaaccgcacg
    10801 ccactttatc cgggagattt tctcccatcc ttcaatccag aatgcaggca ttccgtggaa
    10861 aaaagatttc cgggaatgaa ttcgaatccc tttatgcgct gccctccgtc ctggaagccg
    10921 tcctggtttc cattcaattg cagcaggcca ttagaacaga aggattggcg gaagcgaaga
    10981 tcggccttca tgcggacctg tccctgaacg ctgatcaacc ggtccggccg ctggacctgg
    11041 agatcctgga ccgtatcctt gaactgggcg cccccaatac ggtagtgatg tctgaacccg
    11101 tggcggaaca aataaataat caccctgaat tccggacggg gaagattggt aaaattcacc
    11161 ataaggccta tgcaggaggt ttaaacattt attcgcttca tggttatggg ctcagcagcc
    11221 ttccggcttc ccgtttttat gaaaggctga aagccaggct gaaaatcgcc tccctatttt
    11281 aatccaaaca gttgacctga cgaggtcccg aaaacagcat ttatggcggc ctgtcgcaaa
    11341 tgccccgtgc tttgtcgttt tttcactttt gcccgttctg agatatccgt taggtttgta
    11401 tgaaaataac tccataaacc aaaattgaat cttatgtcaa gcactgaaaa aaccgttttg
    11461 accattgtgg ccggcatcca ggctcccgtt gaaaaagtat gggcatgctg gacggaaccc
    11521 aggcatatca ttaaatggaa tgccgcctcc ccggactggc aatgccctta tgcggaaaac
    11581 gacctgcgcc cgggaggaaa attcatgtcc cgcatggagg ccaaagacgg gtcttatggc
    11641 tttgattttg cgggagaata cacccgggtg gatccacttc gtttgattga atataccctg
    11701 ggggatggcc gaaaggtcca aaccaccttt gtttcctcag gcggggaaac ccggatcatt
    11761 tcccacttcg aagctgaaaa ttcgaattcc ctcgaaatgc agcagcaagg ctggcaggcc
    11821 atcctcaata attttaagaa acacgcggaa tccgcgggta cggaaactat gcaatttgag
    11881 gtcaagatcc aggcgccggt tgaaaaagta tattccaata tgctggacga tatacagtac
    11941 agggcctgga cttcggtttt taacccgacc tcccattata aaggctcctg ggaaaaaggc
    12001 tccaagatct tatttatcgg caccgaccag gacggcaata ctggcggcat ggtcagccgg
    12061 atccgggaaa atatacccaa tacctttgtc agcattgaac acctcggtgt agtgcagggt
    12121 gagcaggaaa tcaccagcgg cccgcaggtg gaagcctggg caggcgccct ggaaaattat
    12181 actttccgcc gggacggaac cggtacccgc ctggtggtgg atctggaggc caacgaggaa
    12241 tacaagtctt atttcgaaga acactggccc aaagccctgg acaagctgaa atccatttgt
    12301 gaaggttatc ccgcttctgc taactgaacc acctattgat taaacgatta aaaaagctca
    12361 gcatatggcc agtgtaagca cctatttaaa cttccagaga gaaacggaag ccgcctttaa
    12421 tttttacaaa tccgtattcg gaggtgaatt catcggagcc gtctcccggt tcagggatat
    12481 tcctcccacc gaaggcatgc ccgaattgcc ggaagcggat aaagacctga tcatgaacat
    12541 cgccctgaag atccttgggg gccacctgct catgggcacc gatgcgccgg agtccatggg
    12601 tttcaaactc cgtcagggaa ataatgtata tatcaccctg catcccgata cgcgcgccga
    12661 aacgaaaaaa ctgtttgatg cgctttcgga cggtggcgtg gtcgaacagg aattgcagga
    12721 tatgttctgg ggggattatt acggcagctg cagggacaaa ttcggggtac aatggatgtt
    12781 caattgttcg gaaaaggata accgctgatt tcttagtgcg gtagtattca tcttctcctg
    12841 caccccgtca gggtgctccc cgcctgaagg attggccatg gtcgtatctt ttcccgtgag
    12901 gatcacccgt cctaagatat cgtatttggt aaatatccgg aaaccattgg gatcggatcg
    12961 cactacttcg cggtccaggc ggtcgt
//
LOCUS       DAOQXT010000122        12740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5413_length_12740_cov_6.335751, whole genome shotgun sequence.
ACCESSION   DAOQXT010000122 DAOQXT010000000
VERSION     DAOQXT010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5413_length_12740_cov_6.335751"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKM27_16615"
     CDS             <1..589
                     /locus_tag="PKM27_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNR08944.1"
                     /translation="LVKPEVRTLAAERGYEELAKKAESYEICFVPDNDYRGFLKRKRP
                     ELESALKGGLYVDQDGKVLGTHEGYPFYTIGQRKGLGVHLGQPMFVTDIQPDQNIVVL
                     GHEDDLQRSGMRVYKTNWQKKEEPQSMECIVKIRYRDPGTWGILDSGPNHSANVLFHV
                     PAKSITPGQSAVFYEGDDVVGGGIIQSGYTFDPSE"
     gene            586..1329
                     /locus_tag="PKM27_16620"
     CDS             586..1329
                     /locus_tag="PKM27_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08945.1"
                     /translation="MKHLGLMLFLLLPLSGCVSNETEETDILSDQKYFPLRKGKFIEY
                     VADSIVFRQGSLLDSTRFEAREEILTEGQDSLGPFYTILRSHRPGAGEPWVPYASYTA
                     RVFQNRAIVNEANVHFIKLVFPLLPGISWNGLGLVRPDQVFNVEGESVEIYRDWENFK
                     AGESPGGFTSGNLVFDDVVSILQTDEENLLAKRYSLEKYAAGTGLVYKEMIILDCNNN
                     VNDCAAVNLPWSQRATKGYIYRQTITRHN"
     gene            1371..1862
                     /locus_tag="PKM27_16625"
     CDS             1371..1862
                     /locus_tag="PKM27_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08946.1"
                     /translation="MRKTTGYQGKVLAEPYAGYEETWNHLSKCLIRVHGVWAPFFVLH
                     KEERRDGMEVLFDDLRDDKEAKSLVNEEIFVHPQDILNNESAEEEDDELAEWIGFTVL
                     DQTAGFTGRIEAIDEMPTQLLALVRSGEREYHIPLVEEFIDEVDLKNRILFMQLPPGL
                     LAL"
     gene            complement(1870..2217)
                     /locus_tag="PKM27_16630"
     CDS             complement(1870..2217)
                     /locus_tag="PKM27_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNR08947.1"
                     /translation="MKHKKNIIRILKKGGYSVIYSALLLYYAFRRKDLPRWARSIIIG
                     GLGYLLSPIDTIPDLTPLLGYTDDLGILSYALVLIAAYINDEVREKAKARFHKLVREP
                     MSEQAIHSVESFL"
     gene            2429..3220
                     /locus_tag="PKM27_16635"
     CDS             2429..3220
                     /locus_tag="PKM27_16635"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR08948.1"
                     /translation="MEKHILPDPVKGMIYVPPGPLDLIDPNSSTTLRVYVQGFFMDST
                     EATVAEFERFVKETGYKTDAEKFGNAGVFNMDDRTWGLVDGADWRHPRGKSKPAAGKS
                     EPVTQLSWYDARAFCAWQGKRLPHELEWEHAARNGRNDQDLYPWGGDWDSGGKIRGNV
                     WQGAFPEVFKNLDGFETTAPVGSFEPSPLGFLDLSGNVWEWCDNWRFEYDQLIQGAFT
                     LSTEKAMRGGSFLCAPNYCHGYKISSRSFTTPETSLFHIGCRCVD"
     gene            complement(3217..6528)
                     /gene="mfd"
                     /locus_tag="PKM27_16640"
     CDS             complement(3217..6528)
                     /gene="mfd"
                     /locus_tag="PKM27_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNR08949.1"
                     /translation="MTGSMESFCLVATILNHPGTHLIVARSKEAAAYLLNDLQGLAGS
                     QILIFFLPDSFRLPGKFEEIKQSQVVERTECINQLLSPGNTSSCIVTYPEALFEKVVA
                     PAALEKSRILIEKGHELDLDFALDLLTEYGFRRVDFVTQPGEFSIRGGIVDIFSFAND
                     YPYRVELADTEVETIRIFDPETQLSVRNLIKISILPNIHTEFSQVEKVSLLDILPKGS
                     VLWMDDLQFTLDRLQDCFDKSQAVRVFEKEEEQQTAFAKIFREKAIVYPREIMDKLPD
                     HSLIFYREKPSSELSGIPQLRFSSRPQPSFNRNFDLLIQDLKKEEASGFEPFIFSDNP
                     RQLDRLDAILRDLKAGVRLQPVPAAIHQGFIDTDLKLLCYTDHQIFERFHRYRLKEGF
                     QKDTAMTMRLLRDLQPGDYVTHIDHGIGRFSGLEKIEINGKTQEVVRLVFKNNDLLYV
                     SIHSLHKISKYMGKEGVEPVLNKIGSDTWSNLKRKAKRQVKDIAQELIKLYAQRRASK
                     GHAFAPDNYMQHELEASFIYEDTPDQLKATQEVKQDMEKEYPMDRMVCGDVGFGKTEV
                     AIRAAFKAILDGKQVAVLVPTTILALQHFKTFSERLKEFKVSVDFINRFRSTKEKNEI
                     LEKTKEGKIEILIGTHAILSQDVQFKNLGLLIIDEEQKFGVAAKEKLRKRKVNVDTLT
                     LTATPIPRTLQFSLLSARDMSIMRTPPANRQPVHTERMTFNEEAIRDAIYYEINRGGQ
                     VFFVHNRVASLPELTEMLREQCPGVEITMAHGQMEPKNLENALLDFIDRKTDVLVSTN
                     IIEMGLDIPNANTIFIHNAHQFGLSDLHQLRGRVGRSNKKAYCYLIAPPLSTLTSEAR
                     QRLKTIEELTDLGSGFYVSMRDLDIRGAGNMLGAEQSGFISEIGFDTYQKILDEAIQE
                     LKEEDFKEVFREELANKKEFVRDCNIDMDAELLIPDTYIQNTPERLSLYTELDAINDE
                     AGIEKFTVKLKDRFGPIPGQMLDLFEGLRIRWTGKRLGMERIIFKNNRLRCYFPGNAK
                     SSYYETPFFQELLKIISTTEYKKEWHLKQTSDSLILGRDGIKNVRTVKKVLEELLAGV
                     ESRLIPRPSPLTPHT"
     gene            6850..7569
                     /locus_tag="PKM27_16645"
     CDS             6850..7569
                     /locus_tag="PKM27_16645"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNR08950.1"
                     /translation="MKTKFFYLLALMSGLAFLPSCEIIEGEGPVVTEFRNKNNFDGIK
                     ISICGQVNFQVAPQYKVELQAPRNILDELETKVESRSLVVKWDNNVRIRDCEDVTVNL
                     AGPDLKYIFNSGSSDIRVTGAVKHPNLELIVSGSGSIDMDYAEIAQEIETQVSGSGHV
                     EIKDGSADESKLHTSGSGYIDMRNVEVKEADTHISGSGMITVNVEDYLEAHISGSGDV
                     YYLGEPRINSKVSGSGKVRKL"
     gene            complement(7639..8157)
                     /locus_tag="PKM27_16650"
     CDS             complement(7639..8157)
                     /locus_tag="PKM27_16650"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR08951.1"
                     /translation="MTLPFFQDYARYTIWANEEVCGWLEQISDEQWTRPVVSSFGTLE
                     ATVLHIAGAEKIWHDRLAGIKPASWLPAYFKGSKQEAIDTWRTHSRSFLDLITQLDPS
                     VLNSNLAFKRLSGEPDEMPYFQILTHVFNHSTYHRGQIILLLRQLGFDRVNSTDITAF
                     LRKKKKLEVPQS"
     gene            complement(8716..9807)
                     /locus_tag="PKM27_16655"
     CDS             complement(8716..9807)
                     /locus_tag="PKM27_16655"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNR08952.1"
                     /translation="METASLSTRAFMEKGFTKPYLIAGPCSAETEEQVMATAAQIAAE
                     GIKPDLFRAGIWKPRTRPGSFEGIGRPGLNWLKKVKEEYGWKTTTEVANTQHVFDALK
                     AGVDVLWIGARTTVNPFSVQEIADALVGVDIPVLVKNPINPDLSLWIGSLERLKKAGI
                     TRLGVIHRGFSAFGKSIYRNTPRWELPIELIRQYPDLFIICDASHICGNRPGLWEVAQ
                     RAMDLNYDGIMIETHCNPDAAWSDAAQQVTGSGLKNILDRLVVRQEKSADPKFIDNIE
                     DIRHQIDVIDNELLNILGERMKLADKIGMFKKMNNISILQSSRWNEIIQKSQEKGKAL
                     GLSEDFITKVLTAIHEESIEHQEKIMIES"
     gene            complement(9839..10684)
                     /locus_tag="PKM27_16660"
     CDS             complement(9839..10684)
                     /locus_tag="PKM27_16660"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNR08953.1"
                     /translation="MSRLCLIGVGHIGGSLALAGKKAGLFSKVTGVDVDPDNLEKALS
                     LGLIEEALPLAEAINGSDLVVLAVPVNVMVNLIPQVLDLVDDQLVTEVGSTKTAILKS
                     IAQHPKRKQFVASHPMAGTEFSGPESAQANLFTGKTCVLVDTADSDPEKVKQLENVYS
                     EINMRLVYLDSESHDLHAAYVSHISHISSFALALTVLAKEKDENQIFQLASGGFSSTV
                     RLAKSSPDMWIPIFEQNRDNVLDVLDEHISTLSRFRSLLIKRDFKTFRHLIEEANQIK
                     KILKT"
     gene            complement(10681..11853)
                     /locus_tag="PKM27_16665"
     CDS             complement(10681..11853)
                     /locus_tag="PKM27_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR08954.1"
                     /translation="MTIKPAHRINTVEEYYFSSKLREIAGMRAGGADVLNLGIGSPDL
                     PPHPSVIATLHEWALRPDAHGYQGYQGRPELRLAMANWYRLYFGVSLDPEKEVLPLLG
                     SKEGIMHISMAFINPGDRVLVPDPGYPTYSAATKLAGGIPETYDLLPENNWKPDMRAL
                     ENIAPGEIKLMWLNYPHMPTGQAADLKTMETLLNWARSRNILLVNDNPYSFILNRNYL
                     SILSLDGAREQSIELNSMSKAQNMAGWRVGMAISNPEFINHIIRFKSNMDSGMFLPVQ
                     MAAVKALELGQDWYDQLNEIYTGRKDLAAAILKAAGCTLQDNQQGLFLWGRIPDKYED
                     SYALSDRILRQAHVFITPGGIFGNNGNQYLRISLCSKPEVLQQGLDRIQKLKLQAS"
     gene            complement(11843..12715)
                     /locus_tag="PKM27_16670"
     CDS             complement(11843..12715)
                     /locus_tag="PKM27_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNR08955.1"
                     /translation="MTKLNTDQTKPETLRVAIQGYRGAFHDIAVQRYFKGHPLEVAET
                     ETFEEIVQLVEKKQVDFGAMAIENTIYGMLMPNYNLLNKARVSIHGEVYLRIQQNLIA
                     WPGQKPEDLKEVHSHPIAIEQCREYFKAWPHIKLIESVDTAWSVREVKEKKLKSRGAI
                     GSTLAAQLYGLEILVPSIETNKKNFTRFLIIERKGSSNPLPHSNKASVIFSLTHEVGS
                     LHQVLSILASQEANLTKIQSIPILGKPFEYAFWVDLTADHMQKLLTAIDSIAPMIHDL
                     KVLGIYPKGAHYDH"
BASE COUNT         2992 a         3033 c         3371 g         3344 t
ORIGIN      
        1 gctggtgaag cctgaagtcc ggaccctggc ggcagaacgg ggctatgaag aattggccaa
       61 aaaagcggag agttacgaga tctgttttgt gccggacaac gattaccgcg gatttttaaa
      121 acgcaaacgt ccggaactgg aatccgcttt gaaagggggg ctttacgtcg atcaggacgg
      181 gaaggtcctg ggtacgcatg agggctatcc tttttacacc attggccaga gaaaaggcct
      241 gggcgttcat ctcggacaac cgatgttcgt gacggatatt caaccggacc aaaatatcgt
      301 ggtcctgggt catgaagacg atctgcaacg cagcggcatg cgcgtttata aaaccaactg
      361 gcagaaaaaa gaagagcctc aatccatgga atgcattgta aaaataagat accgggaccc
      421 cggaacctgg ggtatcctgg acagcggacc gaaccattct gccaatgtcc tttttcatgt
      481 acccgccaaa tccatcaccc cgggacagtc cgccgttttt tatgaaggag atgatgtggt
      541 aggcggaggg atcattcaat ccggttatac ctttgatccc agcgaatgaa acacctgggt
      601 cttatgctgt tcctgttgct tcctttgtcc ggatgcgtca gcaatgaaac agaggaaacc
      661 gatattttat cggatcaaaa atattttccc ctccggaaag gcaagttcat agaatacgtg
      721 gccgattcca tcgtattccg ccaaggcagc ctgctggatt ccacccgctt cgaagcacgg
      781 gaagaaatct tgacagaagg ccaggacagt cttggtccat tttataccat tctgcgctcg
      841 caccgccctg gcgccggtga accctgggtc ccttatgcca gttatacggc cagggtattt
      901 caaaacaggg ctatcgtaaa tgaagccaac gtccatttca ttaaattggt cttcccgctc
      961 ttgcccggca tttcctggaa cggcctgggc ctggtccggc ctgaccaggt atttaacgtt
     1021 gaaggggaat ccgttgaaat ataccgcgat tgggaaaact tcaaagccgg tgagtccccg
     1081 ggcggtttta ccagcggtaa cctcgtgttt gacgatgtgg tgtctatcct tcaaaccgat
     1141 gaggaaaacc tgctcgccaa gcggtacagc ctggagaaat atgcggcagg aaccggcctg
     1201 gtctataagg aaatgatcat cctcgactgc aacaacaacg tcaacgattg tgcagccgtc
     1261 aacctgccct ggagccagcg ggcaaccaag ggatatattt acaggcaaac catcacccgt
     1321 cataattagt ggaaccttcc tttgaatctg atttaagcac tatcggtgtc gtgcgcaaaa
     1381 ccaccggtta ccagggcaaa gtccttgctg aaccttatgc gggttacgaa gaaacatgga
     1441 accatctgtc aaaatgcctg atccgggtcc atggagtctg ggcgcctttt tttgtccttc
     1501 ataaagagga acgccgggac gggatggaag tcttatttga tgatctcagg gatgacaaag
     1561 aagccaaaag cctggtgaat gaggagatct ttgtccatcc gcaggacata ttaaataatg
     1621 aatccgcgga agaagaagac gacgaactgg ctgagtggat cggttttacc gttttggacc
     1681 aaaccgccgg ttttaccggc cggatcgagg ccatcgatga gatgcctaca caattgctgg
     1741 cccttgtccg ctcgggggaa agagaatacc atatcccgct tgtggaagaa tttattgacg
     1801 aggtagacct taaaaacagg atccttttca tgcagttgcc acccgggctg ctcgctcttt
     1861 aaagatcttt taaagaaaac tttcgacgga atgaatggcc tgttcgctca tgggttcacg
     1921 gaccaattta tgaaaacggg cttttgcttt ttccctaacc tcgtcattga tataggccgc
     1981 gatcaggacc agcgcatagg acaatatgcc caaatcatcg gtatatccca agagcggtgt
     2041 aagatcagga atggtatcta tggggcttaa aagatagccc aaacctccga ttataatact
     2101 tctggcccat cgcggtaaat ccttgcgcct aaaggcatag tagagcaaca aagccgaata
     2161 aatcacagaa tacccgccct ttttgagaat ccggataata ttttttttat gcttcattta
     2221 atagtataac aaataaccat tggctgatag tttctcaaaa agcagtcgcc tggcttaatt
     2281 ttgtggaatg cttagccaga ctggtcgatt ttttttcgga tcgctgattt taacgatcct
     2341 gatagcatgt aaaaaaggcg aatcagaagg cttaaggccg gtccagggca tatttgaact
     2401 gccggttaca gtttcaatgg ttccctccgt ggaaaaacat attcttccgg accctgtcaa
     2461 aggcatgatc tatgtgccac ccgggcccct ggacctcatc gatccgaatt caagtaccac
     2521 cctaagggtt tatgttcaag ggttctttat ggacagtacg gaggcgacgg tggccgaatt
     2581 tgagcggttc gttaaggaaa cgggctataa gacggatgcg gaaaaatttg gcaatgcagg
     2641 ggtatttaat atggacgacc ggacctgggg attggtggac ggcgccgact ggagacatcc
     2701 gagagggaaa tccaaaccgg cagccggaaa atcagaaccg gtgacccagt tgtcctggta
     2761 tgacgcccgg gctttttgcg cctggcaggg gaagcgtctt ccccatgagc tggagtggga
     2821 acatgcggcc cgcaatggcc gaaacgacca ggatctctat ccctggggag gggattggga
     2881 ttcgggtggt aagatcaggg gcaatgtatg gcagggggct tttcctgaag ttttcaaaaa
     2941 cctggacggt tttgagacca ccgcaccagt aggcagtttt gagcccagtc ctttgggttt
     3001 tttggatctt tccggcaatg tttgggagtg gtgtgacaac tggcggtttg aatatgacca
     3061 gttgatccag ggtgctttta ccctctccac ggaaaaagct atgcgcggag ggtctttcct
     3121 ctgtgccccg aactattgtc atggatataa aatctcctca agatcattta ccacaccgga
     3181 aaccagtttg tttcatatcg ggtgcaggtg cgtggactag gtatgaggag tgaggggtga
     3241 ggggcggggg atgagccggc tctcgacacc agccaataat tcttcgagta cttttttgac
     3301 cgtccgcaca ttttttatcc catccctgcc gaggatcagg ctatcgctgg tctgcttaag
     3361 atgccattcc tttttgtatt ccgtggtgga aatgattttg agcagctcct ggaaaaaggg
     3421 agtttcgtaa taactggatt tggcattacc cgggaaataa caacgcagcc ggttgttttt
     3481 aaaaataatg cgttccattc ccagtctttt tccggtccaa cggatccgca ggccttcgaa
     3541 caaatcgagc atctggccgg gtatgggacc gaagcggtct ttcagtttta ccgtaaattt
     3601 ttcaatcccg gcctcatcgt tgatcgcgtc cagttcagta tagaggctga gtcgttcagg
     3661 cgtgttctgg atataggtat cagggatcag taattccgca tccatgtcaa tattgcaatc
     3721 ccggacgaat tcctttttat tggccagttc ttccctgaaa acttctttga aatcttcttc
     3781 cttcagttcc tggatggctt cgtccaggat cttctggtag gtgtcaaagc cgatctccga
     3841 aatgaagccg ctttgttctg ccccgagcat attgccggca ccccggatat ccaggtcgcg
     3901 catcgacaca taaaatccgg agccaaggtc cgtgagttcc tcaatggttt ttaatctttg
     3961 gcgggcttca gaagtcaggg tggacaaggg aggggcgatc aggtagcagt aggctttttt
     4021 attggacctg ccgacccgtc ccctcaactg gtggaggtcg ctgagcccaa actggtgggc
     4081 attgtgaata aaaatggtat tggcattggg tatgtccagg cccatttcga tgatattggt
     4141 gctgaccagg acatcggtct tccggtcaat aaagtccagt aaggcgtttt ccaggttttt
     4201 aggttccatt tgtccgtggg ccatggtgat ttccactccc gggcattgtt ccctcagcat
     4261 ttcagtgagc tcgggcaggg aagccacccg gttgtgcaca aaaaaaacct ggccgccccg
     4321 gttgatttca taatagatgg catcgcgaat ggcttcttca ttaaaagtca ttcgttcggt
     4381 gtgaacgggc tggcggttgg ccggcggggt tcgcatgatc gacatatccc gggccgaaag
     4441 gagggaaaac tgcagcgttc tcgggatggg ggtggcggtg agggtcaggg tgtcgacatt
     4501 cacctttctc ttgcgcaatt tctcttttgc tgccaccccg aatttttgtt cttcgtcaat
     4561 gatcaggagg cccaggtttt taaactggac gtcctggctg aggatcgcat gggtgccaat
     4621 gagtatctct attttcccct ctttggtttt ctcgaggatc tcattttttt ccttcgtgct
     4681 ccggaaccgg ttgataaaat ccacactgac cttgaattct ttgagccgct cgctgaacgt
     4741 tttaaaatgc tgaagagcga ggatggtcgt cggtaccaat accgccacct gttttccgtc
     4801 cagaatggcc ttgaaggctg cgcggatggc gacctcggtt ttcccaaatc ccacgtcgcc
     4861 gcagaccatt cggtccatgg gatattcttt ttccatgtcc tgttttacct cctgcgtggc
     4921 cttcagctgg tcgggcgtgt cctcataaat gaaggaggct tccagttcgt gttgcatata
     4981 attgtccggg gcaaaggcat gtcccttgct ggccctgcgc tgggcataga gtttgatcag
     5041 ttcctgggcg atgtctttga cctggcgctt ggctttcctt ttcaggttgg accaggtatc
     5101 gctgcctatc ttattcagca ccggttcgac cccctccttg cccatgtact tgctgatttt
     5161 gtgcagggag tgaatgctta catacaacag gtcgttattc ttaaacacca gccgcaccac
     5221 ctcctgggtc ttgccgttga tctctatttt ttccaatcct gaaaaacgcc cgatcccatg
     5281 atcgatatgg gtgacatagt ccccgggttg aaggtcccgg agcaggcgca tggtcattgc
     5341 ggtgtctttc tgaaatcctt ccttgagccg gtaccggtgg aaccgttcaa agatctggtg
     5401 gtccgtataa caaaggagtt tcaggtccgt atcgataaag ccctggtgaa tggctgcagg
     5461 aaccggttgc aggcggaccc cggccttaag gtcccggaga atagcatcca ggcgatccag
     5521 ttgccttgga ttgtctgaaa aaataaaagg ttcgaaaccg gatgcttcct ccttcttcag
     5581 gtcctggatc agcaagtcaa aattccggtt aaaactgggc tgcggccttg atgaaaaacg
     5641 aagttgcggg atacccgata actctgagga tggcttttcc cggtaaaaga tcagggaatg
     5701 gtccggaagt ttatccataa tctccctggg atagacaatg gctttttccc tgaaaatttt
     5761 ggcgaaggct gtttgttgct cctcctcttt ttcaaaaacc cggaccgcct ggcttttgtc
     5821 aaaacaatcc tgcagccggt caagggtaaa ctgcaggtcg tccatccaga gaacagatcc
     5881 tttgggcagg atgtcgagga gggagacttt ttccacctgg ctgaattcgg tgtggatatt
     5941 gggtaaaatg ctgattttga tcaaattccg gacagacaac tgtgtttccg ggtcaaaaat
     6001 ccggatggtt tccacctcgg tgtcggccag ttccacccgg tagggataat cattggcgaa
     6061 ggagaaaata tcaacgatcc cgccccggat ggaaaattca cccggctggg tcacaaaatc
     6121 tacccttctg aatccgtatt ccgtgagcag gtccagggca aaatccagat cgagttcatg
     6181 tcctttttcg atcaggatcc tgcttttttc cagcgcggca ggggccacca ctttctcaaa
     6241 cagagcttcc gggtaggtaa cgatgcagga ggaggtgttg ccggggctga gcagctggtt
     6301 gatgcattcc gtcctttcaa ccacctggct ttgtttgatt tcttcaaatt tgccgggcag
     6361 acggaatgag tccggcagga aaaaaataag gatctggctt cccgcaagtc cttgaagatc
     6421 atttagtaaa taagccgctg cctccttgga tctggccacg atgaggtggg tgcccgggtg
     6481 gttgaggatg gtggcgacaa ggcaaaagga ttccatcgag ccagtcatac cggagagata
     6541 taaccgccct ggcttcgttg ataaaacagt ccctacctcc cttatccggg cgtcctgccg
     6601 gtatatcgcc agcaagcggc tcaggttttc cacgggcgca aagataaaga agcggctggc
     6661 aggctgcagc caaaagaatg gaaatgaaat tgaaaaatgg agggccccgc ggtcgatcgg
     6721 cccatttcat tggtcgtctt tccggattct ttaaaccgtt taattttcat tccgtgcaac
     6781 taagccggca aatttgtctc tttattgatt gaaaattaat tcaaaactga tactgtttaa
     6841 attttaatta tgaaaacgaa atttttttac ctccttgctt tgatgtccgg actggccttt
     6901 ttgccttcct gtgaaatcat tgaaggggaa gggcctgtcg tgactgaatt ccgcaacaaa
     6961 aataattttg acgggatcaa gatcagcatt tgcggacagg ttaatttcca ggttgccccg
     7021 caatataagg tggaattgca ggctccccgt aatatccttg acgaattgga aacaaaggtg
     7081 gaaagcaggt ccctggtggt caaatgggac aacaacgtcc gtatccgcga ttgtgaagac
     7141 gtgaccgtca accttgccgg cccggacctg aaatacatat ttaacagcgg ctcatcggac
     7201 atccgcgtga ccggcgccgt taaacatcca aatctcgagt tgatcgtaag cggttccggc
     7261 agcatcgata tggactatgc agaaatcgcc caggaaatcg agacccaggt gagcggttca
     7321 ggccatgtgg aaataaaaga cggttcagcg gatgaaagca aactccacac cagcggttcc
     7381 gggtatatcg acatgcgcaa cgtggaagta aaagaggcgg acacccatat cagtggctcg
     7441 ggtatgatta ctgtgaatgt tgaagactat ctcgaagccc atatctccgg ctccggtgac
     7501 gtctattatc tgggagagcc caggatcaat tcaaaggtat ccggatcggg caaagtcagg
     7561 aaactttaat ttaagcactg gttcgaaggg cgagcgtcaa aagcccacgt cagggcgctg
     7621 tgaatattga gctggaagtc acgattgtgg gacttccagc ttcttttttt tcctgagaaa
     7681 tgcggtgata tcggtactgt ttacccggtc aaagcccaat tgcctcagca aaaggatgat
     7741 ctgaccccgg tggtaggtgg agtgattgaa tacatgggta aggatctgaa aatagggcat
     7801 ctcatccggc tccccactga ggcgtttaaa tgccaggttg gagttaagca ccgaaggatc
     7861 gagttgggta attaaatcga gaaagctcct ggaatgggtc ctccaggtat caatggcctc
     7921 ctgcttcgat cctttaaaat aggccggcag ccaggaagcc ggttttattc cggccagacg
     7981 gtcgtgccat attttttcgg ccccggcgat gtgcaaaacc gttgcttcca gggtgccaaa
     8041 actggatacc acgggcctgg tccattgttc atcgctgatt tgttcaagcc agccgcatac
     8101 ttcttcattg gcccaaatgg tatacctggc gtaatcctgg aaaaagggta gtgtcatgat
     8161 gcaatcgatt gaacggtaaa aatagatagg atttctagga tttctaggat gggtaggact
     8221 aggattggtg ggatttctag gatgggtaga ctaggattgg taggatttct aggatttcta
     8281 ggatgggtag actaggattg gtaggatttc taggatgggt aggactagga ttggtaggat
     8341 ttctaggatt tctaggatgg gtaggattag gattggtagg atttctagga tttctaggat
     8401 gggtaggatt aggattggtg ggatttctag gatgggtagg actaggattg gtaggattgg
     8461 taggatttct aggatttttg attggtagga ttccaaggat tggcagatta ggctgtaagg
     8521 attcggttca gtttttgctg aacaaatgtg agcacatccg accgccttag gaaccaggac
     8581 aggcttttta attaagccgc gcccaacgaa ataaaatctt acctactaat cctaccaatc
     8641 ctaccaatcc taccaatcct actaatccta ctaatcctat aaatcctata aatcctataa
     8701 atcctattaa tcctatcacg attcgatcat aatcttctcc tgatgctcaa tggactcttc
     8761 atgaatggcg gtgagcacct tagtgataaa gtcttcactc aatcccagcg ccttgccttt
     8821 ttcctggctt ttctggatga tctcattcca gcggctggat tgaagaatac tgatattgtt
     8881 catttttttg aacatgccaa ttttgtctgc cagtttcatc cgttcgccca ggatgttgag
     8941 caattcgttg tcgatgacat cgatctggtg acggatgtcc tcaatattat ctatgaattt
     9001 cggatccgct gacttttcct gccggacgac aagtcggtca aggatgtttt tgaggccgct
     9061 cccggtgact tgctgggcgg catcgctcca ggccgcgtcc gggttgcaat gggtttcgat
     9121 catgatcccg tcataattga ggtccatggc acgctgggcg acctcccaaa gtccgggccg
     9181 gttaccacaa atatggctgg catcacagat gataaaaaga tcggggtatt gcctgatcag
     9241 ttcgatcggc aattcccacc tgggggtatt gcggtagatg gatttgccaa aagcactgaa
     9301 tccccggtgt atgacgccca ggcgggtgat gccggctttt ttcaaacgtt ccagggaccc
     9361 gatccaaagg ctcaggtcag gattgattgg gtttttgacc agtacgggga tgtcgacccc
     9421 gaccagggca tcggcaatct cctggacgga aaatggatta acggtagtcc ttgccccgat
     9481 ccataaaaca tcgacccctg ctttcagtgc atcgaacaca tgctgggtat tggcgacctc
     9541 ggtagtcgtc ttccagccgt attcctcctt gacttttttc agccagttca atccgggcct
     9601 gccaatacct tcgaaactgc ccggacgggt gcgcggcttc cagattccgg cacggaacag
     9661 gtccggtttg atgccttctg ccgcgatctg tgcagcggtg gccatgacct gttcttccgt
     9721 ttctgcactg caggggccgg cgatcaggta aggttttgta aatccttttt ccatgaaggc
     9781 cctggtggat agacttgctg tttccatatt ttttgctttg tttatttgat aatgtatttc
     9841 aggttttgag tattttttta atctggttgg cctcttcgat caggtgccgg aaagtcttaa
     9901 aatccctttt gatgaggagg cttctgaaac gggacaaagt gctgatgtgc tcatccagca
     9961 cgtcgagcac gttgtcccgg ttttgttcaa aaatggggat ccacatgtcc ggtgagcttt
    10021 tggccagcct gaccgtagaa ctgaaaccac cgctggccag ttgaaaaatc tggttttcat
    10081 ccttctcctt agccagtacg gtgagggcca gtgcaaaaga actgatgtgt gaaatatgcg
    10141 acacataggc ggcatgcagg tcatgggatt cgctgtccag gtagaccagc cgcatattga
    10201 tctcggaata aacgttttca agctgtttta ctttctccgg atcgctgtca gccgtatcca
    10261 ccaggacgca ggtcttgcct gtaaaaagat tggcctgggc ggattcaggt cccgaaaatt
    10321 ccgtaccggc catagggtgg ctggccacaa attgttttct tttcggatgc tgggctatgc
    10381 ttttcagaat ggcggtttta gtagaaccca cttcggtgac cagctggtcg tcaaccaggt
    10441 caagcacctg ggggatcagg ttgaccatga cgtttaccgg cacagccagg acgaccaggt
    10501 cagaaccatt gatggcttcc gcaaggggaa gggcttcctc gatcagtccc agggatagtg
    10561 ctttttcgag gttatccggg tcgacatcca ccccggtgac tttagaaaac agcccggctt
    10621 tttttcccgc gagggcaagg gacccgccga tatggcccac cccaatgaga cataacctgc
    10681 tcatgatgcc tgcagtttaa gtttttgaat tcggtcgagg ccttgctgca gcacttccgg
    10741 tttgctgcag agagaaatcc tgaggtattg gtttccgttg ttgccaaaaa taccccccgg
    10801 ggttataaag acatgggctt gccggaggat ccggtcacta agggcataac tgtcttcata
    10861 cttatcgggg atcctgcccc agaggaacaa accctgctgg ttatcctgca gggtacatcc
    10921 ggcagccttc aggatggccg cagccaggtc tttccggccg gtatagattt cgttgagctg
    10981 atcataccaa tcctgaccga gttccagcgc tttcacggca gccatttgaa cgggtaaaaa
    11041 catgccgctg tccatattgc ttttaaaccg gatgatgtgg ttgataaact cagggtttga
    11101 aatggccatg cccaccctcc aacctgccat gttctgggct ttgctcatgg agttcagttc
    11161 aatcgactgc tcccgggccc catcaaggga aaggatggac aggtagttgc ggttgaggat
    11221 aaaactgtac ggattgtcgt tgaccaggag gatgttcctg gaccgggccc aattcagcaa
    11281 ggtctccatg gttttgagat cggcagcttg tccggtgggc atatgaggat agttgagcca
    11341 catcagtttg atttcaccgg gagcgatatt ttccagggct cgcatatccg gtttccagtt
    11401 gttttcaggc aggaggtcat aggtctccgg tattcctccg gcgagtttgg tcgcggcact
    11461 gtaagtagga tagcccggat ccggcaccag tacccggtcc cccggattga tgaaggccat
    11521 ggaaatatgc atgatccctt cctttgaacc caggagggga aggacttctt tttcaggatc
    11581 caggctcacc ccaaaataca accggtacca gtttgccatg gccagcctta gttccggcct
    11641 accctggtaa ccttgataac cgtgggcatc gggcctgagc gcccattcat gcaaggtggc
    11701 gatcacggaa ggatggggag gcaggtccgg cgatccgatg ccgagattaa ggacatccgc
    11761 gcccccggca cgcatgcctg cgatctccct gagtttggaa gaaaaataat attcttccac
    11821 ggtattgatc cggtgggctg gtttaatggt cataatgcgc tccttttgga taaatgccca
    11881 gcactttcag atcatggatc atgggtgcga tggaatcaat ggcggtgagt agtttttgca
    11941 tgtgatcggc cgtcaggtct acccagaatg catactcaaa aggtttgcca agaatgggaa
    12001 tggactggat tttggtgaga ttggcttcct gggaagccag tatggaaagg acctggtgaa
    12061 ggcttcccac ttcatgggtg agggaaaaaa tcacggaagc tttgttggaa tggggcaggg
    12121 gattcgaact gcctttccgc tcaatgatga ggaaacgggt gaaatttttc ttgttggttt
    12181 caatcgacgg tacgaggatt tccaggccgt acagctgtgc ggcgagggtg ctgccaatgg
    12241 ctccccggct tttcagcttt ttttccttca cctccctgac gctccaggcg gtatccacag
    12301 actcaatcag tttgatatgg ggccaggctt tgaaatattc ccggcattgt tctatggcga
    12361 tggggtggga atgcacctct ttgaggtctt ccggcttctg gcccggccag gcgatcaggt
    12421 tctgctgaat ccggagataa acttcaccat gtatcgatac cctggctttg ttgagcaggt
    12481 tgtagttagg catcagcata ccgtaaatgg tattttctat cgccatcgcc ccaaaatcca
    12541 cctgtttttt ttccaccagt tgcacgattt cttcaaaggt ttcggtctct gccacctcca
    12601 ggggatgacc cttaaaatag cgctggaccg cgatatcgtg gaaagctccc cggtagccct
    12661 ggatggctac ccgcagggtc tccggtttgg tctggtctgt attcaatttt gtcacaacag
    12721 tttcttaaaa cactgaaggg
//
LOCUS       DAOQXT010000123        12516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5522_length_12516_cov_6.961801, whole genome shotgun sequence.
ACCESSION   DAOQXT010000123 DAOQXT010000000
VERSION     DAOQXT010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12516
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5522_length_12516_cov_6.961801"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="PKM27_16675"
     CDS             <1..1524
                     /locus_tag="PKM27_16675"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR08956.1"
                     /translation="WTWSDRGNRGIYDQDKTNKLLEFYMNYGPKFGKYALDLMAGYSW
                     QRFYKQAHFQTTNFIGNKILTPENYNPAEYFLVSLFGRANLNIADRYLLTFTLRRDGT
                     SRFSEDTRWGLFPAGAFAWKILNNSQGALSNLKLRLGYGVTGQQSISDNDYYNYLPRY
                     LSSFENARYQFGNEFVTTLRPQGYDSNIKWEETTTYNAGFDFGLFKDRLTGTFDVYHR
                     VTNDLLNFIPVPAGTNLTNFLTTNVGDLENTGVELALNTIAFRKENQLLEIGVNATVN
                     RNKITRLTATNDPNYVGVFTGGIPGGVGNTIQVHSVGYPANSYWVYEQVYDHNNNPIE
                     GLYVDRNGDGLVTPSDQYRYKKPAPDFTLGLSASYTLGSFDFSTGARASFGNYIYNSL
                     QANGAFGNRTYGSTGILYNSVKDIWRLNLDNVQLFSDHFIQDGSFVRMDHFTAGYLFD
                     KVSFASGLRLSLTVQNPFMITKYKGLDPEVYNPDSPGVDGNIYPRARTFLLGVSASF"
     gene            1547..3742
                     /locus_tag="PKM27_16680"
     CDS             1547..3742
                     /locus_tag="PKM27_16680"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR08957.1"
                     /translation="MMKNINLKTGVMVVVLALTQASCFDDLNTIPLDKDEITSVEVYK
                     DPASYRKVLAKIYAGLALSGQQGPSGLADISGIDEGFSTYLRQYWKAQELSTDEAVIG
                     WNDGNIHDFHQQDWDANNEFITAMYNRIFYQISLCNEFLRETTEEKLASRGISGNILT
                     DISTYRSEVRFLRALSYYHALDMFRNVPFVTEANAVGNFFPDQINKEDLFSFIETEVK
                     EVEPSLKAPKTNEYGRADQAAAWMLLAKLYLNAEVYIGQNRYTDCITYCNKVIGAPFT
                     LSTNYANLFTADNHLSPEIIFPVLFDGINSKTWGGMTFVVHAAVGGSMNVNDFGIDGG
                     WGGTRTTSAIVKKFTAGKGGDVLISPIDPNPNYARIYAPGGYQGWDPAKAKTFSSPKN
                     DGQFQGYIYFDEPTEFKFTTGPNWDENYGDNGADGTLDQNGANFVIPEAGFYKVNADI
                     PGKKYSILKTSWGLIGSATPTGWDSDTDLKYDSKTNTWSLTINLVKGEVKFRANDGWD
                     LNYGDDGSNASLEEGGANIQIPSDGTYIIKLILDRPEYSYHLEKGSIDSRGMFYTTGQ
                     NLEIADISQFTEGYAVSKFKNVTSTGQPGSDKVFVDTDFPLFRLADAYLMLAEAVLRG
                     GAGSSRDQALQYVNALRSRAFKGASGNITDSELNLDFILDERARELLWECTRRTDLVR
                     FGRFSQSTYLWPWKGGVAEGRSTDSKYNVYPLPASDLGANPKLKQNPGY"
     gene            3777..4808
                     /locus_tag="PKM27_16685"
     CDS             3777..4808
                     /locus_tag="PKM27_16685"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HNR08958.1"
                     /translation="MNKLFPIIAVSILVLSSCEKKIDGPYLDFGSDPILFTPATNASF
                     VLAEENAKNVLTPIIWSKTWFGYPAQVTYEVQMDAAGNNFREPAILGTVNQPTLNTVT
                     VERLNAILLGRGFEPGKAASLELRVQAKVSNEVNILYSKASKITLTPYKASLNYPKLQ
                     VPGSHQGWNPADNNTVIYSLKSDDKYEGFLYFPANTEFKYTVGPKWDINYGDTGSDGV
                     LDQNGDNLKAPDAGYYRLRVDLNDKSHSHTLTHWGLIGSATANGWDADQDMTYNASTG
                     IWMITTSLKSGEIKFRANDAWGINFGDNGNNLSLEYDGANIPIGEAGNYEIQLILNTP
                     LYTYQVTKK"
     gene            4903..6798
                     /locus_tag="PKM27_16690"
     CDS             4903..6798
                     /locus_tag="PKM27_16690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNR08959.1"
                     /translation="MFRPLRLLILLTGLQTLSAQEKQHFTKSDTQQVEKLLKSAWSAA
                     RSDPHLSLATLHQADSFIKASRTDYFSSVLIYYYGVVYKNLGAYDSSEIYFDRYIEYS
                     KAKNDLRRLAPVFMAKANLYSDQNRYEKSMDAVTQSLRYFRELNDTAGIITGQSKMGY
                     LLSEIGRFDDAEKYHRFSLDLSTAIRDTVSIAIALTNLGLLFEKNQGYDSALVYFRQS
                     EALSVQLRDTYALADDRYNIGNTLMKQNRLYEAEPYLDQSVLLAQETGTPGQIISARL
                     LLATLRLAQGHREAGMALMEGVLKEFAEEISLKDRSETYRSLYEAYKEKGNISRALHY
                     LESWTSIKDSIFNQDVSRQMNQLEVQYQTTQKDQALQLLNSNHELAQSRLRAARYRIY
                     ALGSGLAAVIVLLFLLWRLWKKTRLQNALIQKSLTEKETLLREIHHRVKNNLQFISSL
                     LNLQARHEKDGQSRSVLREGQNRVKSMALIHQNLYQEHNLTGVEIKSYLENLIKSLFH
                     SYNVSPDLIRLKLDIEPIRLDVDTMVPLGLIINELVSNALKYAFPEGREGWIEVSLRE
                     EGGQLFLRVSDNGIGLKDKNPEKDGHSFGYRLIHAFQTQLNASLELKSDPGTTVTMRI
                     QDYRKAS"
     gene            6879..7343
                     /locus_tag="PKM27_16695"
     CDS             6879..7343
                     /locus_tag="PKM27_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08960.1"
                     /translation="MKIQTLLLGTLITLSACNKDDAPDFSGSYQAFRLRSECTETSKN
                     VSRTFDASTKELCNTVTGGQDCFSLSLTLNADLSYSLTSKTKQIRANGGLVTTKPATD
                     NGTYTVSGNELTLCVGGGSSCITLKTTSVTGELDWLSTNSSGCNYFYTFKKN"
     gene            7402..8025
                     /locus_tag="PKM27_16700"
     CDS             7402..8025
                     /locus_tag="PKM27_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR08961.1"
                     /translation="MSQIRILIIEDEIIIAEDIRDILTALGYEVAGIFTNGDKAVDYL
                     SFHSPDLVLCDISIKGNMDGIQVAEKIRSKKKIPFIFLTSLSDRSTLDRAKKALPYGY
                     IVKPFDERDLLAGIETGLYKFSQELEQLSITREKIDLLVTDPLTDQEYSIVKAMMEGL
                     KTEEICGKMFFSVNTLKYHVKNILSKFQAENRGEIMQKIIAGWMVAK"
     gene            8057..8191
                     /locus_tag="PKM27_16705"
     CDS             8057..8191
                     /locus_tag="PKM27_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08962.1"
                     /translation="MEDHEAEKEIDLKDNLLMASWILDWLRQMDTDLHQAGLDDPGPD
                     "
     gene            complement(8194..9648)
                     /locus_tag="PKM27_16710"
     CDS             complement(8194..9648)
                     /locus_tag="PKM27_16710"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNR08963.1"
                     /translation="MKTKTAIVVCGLFLFASLQCKPKGTTVDMPLKRGADNLAYQWGK
                     ISLELTANDTERFRPRPTVTSRMLGLTWTAIYDAWSRYDSTAKPVYLKQVERRPVSEF
                     TEKNKEITISYAAYEALKQFYFSDTIRLNEYMISLGMDPADASEDPSSPAGIGRLAAR
                     AVFEARKMDGSNQYGEMPDAPQTYQDYTGYQPLNTADDTKDLMRWAPKYFRDSVLGTY
                     APGCLTPHWGKVKTLILDSASQFRSPPPPALGSEQLKNEVREVVEMQARLTDEQRALV
                     EFMRDGPKSVQQAGHWLIFAQDVSVRDQHTLDQDVVMYFLVEAAAMDAFIACWETKMY
                     YDYARPYALVHEYFKNQTIDAWAGPEKGWTKIKGAEWRPYSPDNFLCPPFPAYVSGHS
                     TVSGACSEILRLYTGSDTFGIEVQRVAGALTETNRLGDTVTMRFPTFTNTADQAGISR
                     VLGGYHIQADNVEGLNMGRKVGNEVWNKYLANLK"
     gene            complement(9975..>12516)
                     /locus_tag="PKM27_16715"
     CDS             complement(9975..>12516)
                     /locus_tag="PKM27_16715"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNR08964.1"
                     /translation="FPFQNPDLTMEKRAEDLVSRLSLDQKIAQLMNQAPAIDSLGIPQ
                     YNWWSEGLHGIARAGLATVFPQAIGMAATWDEDLIHRVANVISDETRAKHHEFVRRNK
                     RFIYQGLTLWSPNINIFRDPRWGRGQETYGEDPFLTGTLGVQFIKGLQGDDPVYFKTI
                     ATVKHFAVHSGPEPERHSFDARANLKDMYETYLPQFEMGIREGKAYSLMCAYNRLNGV
                     ACCGSQGLLQGILRKDWNFDGYVVSDCDAIDDIALHHKITATPEEAVALAIKSGTDLE
                     CGRFFKHLRAAVDQNLITEKELDTALKRLFVARMKLGMFDDSSRVRWAQIPYAVVDQE
                     AHRQLALETARKSMVLLKNEGQTLPLKKDLKTVAVIGPNADQWLMLLGNYNGVPADPV
                     TPLRGIREKLPNTKVLFAQGCELAEGMPMFYTIPGEALFNGTSKGLKVEYFGNKDLSG
                     EPLATTTDPILDANWNDRAPRTDMDDDDFGVRWTAELRPQASGIYQLGVITSCNTQLY
                     FEDSVVAKTVYHFRNEYGDPRLRKSLPIHLEKGRSYKIRVDASETFADAQVQLVWAAP
                     KPNLKQEAVALAREADAVVLCMGITPRMEGEEMDIKIDGFRGGDRTKLALPGIQQDLI
                     RSIQALGKPVVLVLLNGSALAVNWEDENLPAILEAWYPGQAAGTAIADVLFGDYNPGG
                     KLPVTFYRSEEDLPPFDQYEITSQTYRYFKGRPLYPFGHGLSYTRFEFSDLEMDTVLA
                     KNQVVSISLKVSNKGGMDGDEVVQVYVKKPDAGEGQPILSLRAYQRVHLKAGESKVVQ
                     LNLRDDAFSQINEKGEKEYLPGNYEIFAGGGQPGYTDQGLRVTVRVLP"
BASE COUNT         3111 a         3389 c         3131 g         2885 t
ORIGIN      
        1 tggacctgga gtgaccgcgg aaaccggggc atttatgatc aggataaaac caacaagctc
       61 ctcgaatttt atatgaatta cggtcccaaa ttcgggaagt atgcgcttga cctcatggcc
      121 ggatattcct ggcagcgttt ttacaaacaa gcccatttcc agaccaccaa ttttatcggc
      181 aataagatcc tgacccccga aaactataat ccggctgaat atttcctggt ttccctattt
      241 ggcagggcca atctcaatat agctgaccgt tacctcctga cctttacctt aaggagagac
      301 ggcacctccc gtttttcgga agacacccgc tggggcttgt ttcccgccgg ggcctttgcc
      361 tggaaaattc tgaacaacag ccagggcgcc ctgagcaatc tgaaacttcg attgggttac
      421 ggggttaccg gccagcaaag catcagtgac aacgactatt acaattacct gccccggtac
      481 ctttccagtt ttgaaaacgc gaggtaccag tttggcaatg aattcgtcac cacccttcgc
      541 ccccagggtt atgactccaa catcaaatgg gaagaaacga ccacttacaa cgcaggcttt
      601 gatttcgggc ttttcaaaga ccgcctcacg gggaccttcg atgtgtatca ccgggtcacc
      661 aatgacctgc tcaattttat acccgtgcct gcgggaacca acctgaccaa ttttctgacg
      721 accaatgtgg gcgatcttga aaacacgggg gtggaactgg ccctgaacac catcgctttc
      781 cggaaagaaa accagttgct cgagatcggc gtaaacgcca cggtaaaccg gaacaagatc
      841 acccggctga ccgcaaccaa tgatcccaat tatgtcggcg tctttaccgg cggtattccg
      901 ggcggggtgg gcaataccat tcaggtccac agcgtgggtt atccggccaa ttcctattgg
      961 gtctacgaac aagtctatga tcacaacaac aatcccatcg aaggattata tgtggaccgc
     1021 aacggggacg gtttggtgac cccgagcgat cagtaccggt ataaaaaacc ggccccggat
     1081 ttcaccctgg gcctcagcgc ctcctatacc cttggttcgt ttgatttcag caccggggcc
     1141 agggccagtt tcggcaatta tatctataac agcctccagg ccaacggcgc ctttggaaac
     1201 agaacttatg gcagcaccgg catcctgtac aactccgtaa aggatatctg gagactgaat
     1261 ttggacaacg tgcagctttt cagcgatcat ttcattcagg atggatcctt tgtccgcatg
     1321 gaccacttca cggccggtta cctttttgat aaagtgagct ttgcgagcgg cttgcgcttg
     1381 tccctcaccg tgcagaaccc gtttatgatt accaaataca aaggactcga ccctgaagtt
     1441 tacaacccgg attccccggg agtggacggc aacatttatc cccgggcgag gacctttctg
     1501 ctcggagtaa gcgcttcatt ttaaaatatt cataaatcac aggaatatga tgaaaaatat
     1561 aaatctgaaa acaggggtga tggtggtcgt tttagccctc acccaagcct cctgttttga
     1621 tgatctcaat accatcccct tggacaaaga tgagatcact tccgtagaag tatacaaaga
     1681 cccggcctcc taccggaagg tccttgccaa gatctatgcc ggccttgccc tgagcgggca
     1741 acagggccct tcgggcctgg cggacatcag tgggatcgac gaaggctttt ccacctatct
     1801 ccggcagtac tggaaggcgc aggaattgtc caccgatgaa gccgtgatcg gctggaatga
     1861 cgggaatatc cacgactttc atcagcagga ctgggatgcc aataacgaat tcatcacggc
     1921 tatgtacaac cggatcttct accagatttc gctgtgcaat gaattcctgc gcgaaaccac
     1981 cgaagaaaaa ctggcttccc ggggcatttc cggaaacatc ctcacggaca tttccactta
     2041 ccgcagcgaa gtccgtttcc tcagggcgct cagttattat cacgccctgg acatgttccg
     2101 gaatgttcct tttgtgaccg aagccaatgc ggtcggaaat tttttccccg accagatcaa
     2161 caaggaggac cttttcagtt tcattgaaac cgaggtcaaa gaggtggagc cctccctcaa
     2221 agcacctaaa accaatgagt acggccgggc cgatcaggct gcggcctgga tgctcctggc
     2281 caaactgtac ctgaatgcag aagtttatat tggccagaac agatataccg attgcattac
     2341 ctattgcaac aaagtgatcg gcgccccctt taccctcagt acaaattatg caaacctttt
     2401 caccgcggac aatcatcttt ccccggaaat cattttcccg gtcctgtttg acgggattaa
     2461 ttcaaaaacc tgggggggta tgacttttgt ggtccatgcg gccgtgggag gcagcatgaa
     2521 tgtcaatgat ttcggcatcg acggaggatg gggcgggacg cgcaccacca gcgccatcgt
     2581 caaaaaattc acggcaggca agggaggcga cgtcctgatc agcccaatcg atcccaaccc
     2641 aaactatgcc aggatctatg cccccggagg ataccagggt tgggatcctg caaaggccaa
     2701 aacctttagc tccccgaaaa acgatggcca gttccagggt tatatctatt ttgatgaacc
     2761 gactgaattc aaatttacga ccggacccaa ctgggatgaa aattacgggg acaacggcgc
     2821 tgacgggacc ctggaccaaa acggggccaa ttttgtcatc ccggaagccg gattttataa
     2881 agtgaatgcg gatatcccgg gaaaaaaata ttccatttta aaaaccagct ggggcctgat
     2941 cggcagcgcc accccgacgg gctgggacag cgataccgac ctgaaatacg attccaaaac
     3001 gaatacctgg tcgctcacca tcaacctggt gaaaggggag gtcaagttca gggccaacga
     3061 cggctgggat ctgaattacg gagacgatgg aagcaatgcc tccctggagg aaggaggggc
     3121 caatattcag ataccatccg acggtaccta tatcatcaaa ctgatcctgg accggccgga
     3181 atattcctat cacctggaaa aaggaagcat tgattccagg gggatgtttt ataccaccgg
     3241 acaaaacctg gagatcgcag acatcagtca gtttacggag ggatatgccg tttcgaaatt
     3301 taaaaacgtt acctccacgg gacaaccggg ttcggataag gtctttgtgg ataccgattt
     3361 ccccctgttc aggcttgccg atgcttacct tatgctcgcg gaagccgtat taaggggagg
     3421 agccggatcc tccagggacc aggccctcca gtatgtcaat gccctccgct ccagagcctt
     3481 taaaggcgca agtggaaata ttacggacag tgaactcaac ctggatttta tcctggatga
     3541 aagggccaga gaactgctct gggaatgcac ccgcaggacc gatctggtcc ggtttggccg
     3601 attcagccaa tccacttatt tgtggccctg gaaaggcggg gttgcggaag gccggtccac
     3661 cgacagcaaa tacaatgtgt accccctccc tgcttccgac ctgggcgcga accctaaact
     3721 aaagcagaac ccgggctatt gatccacatt ttttaaacga ggaaagtgaa aaatatatga
     3781 acaagttatt tccaatcatc gcggtaagta tactggtcct ctcgtcctgt gaaaaaaaga
     3841 ttgacgggcc ttacctggat tttggctcag atcccatcct gttcactccg gccaccaacg
     3901 ccagttttgt actggccgag gaaaatgcca aaaatgtact tacccccatc atctggtcca
     3961 aaacctggtt tggttatccc gcccaggtaa cctatgaagt tcaaatggat gccgccggca
     4021 acaatttcag ggaacccgcc atccttggta cggtaaatca acccaccctc aacaccgtca
     4081 ccgtagaacg cctcaatgcc attttgctcg gcaggggatt cgagcctggc aaagccgcca
     4141 gccttgagtt gagggtccag gccaaagtga gcaatgaagt gaatatcctg tattccaagg
     4201 ccagcaaaat cacactcact ccttacaagg cctccctgaa ttatcccaaa ctccaggtcc
     4261 ccggaagcca ccagggttgg aatccggcgg ataacaacac cgtgatctat tccctgaaaa
     4321 gcgatgacaa atacgaagga ttcctttatt tcccggccaa taccgaattt aaatataccg
     4381 tcggaccgaa atgggatatc aattatggag atactgggtc agacggagtc cttgaccaaa
     4441 acggcgacaa cctgaaagct ccggacgccg gatattaccg tctgagggtc gacctgaatg
     4501 acaaatctca ttcccatacc cttacccact ggggactgat cggttccgca acagccaatg
     4561 gatgggatgc cgaccaggac atgacttaca atgcttctac cgggatctgg atgatcacca
     4621 cctccctgaa aagcggggaa atcaaattca gggccaatga cgcctggggc atcaatttcg
     4681 gggacaatgg caacaatctt tccctggaat acgacggagc gaacattccc atcggagaag
     4741 ccggcaatta cgaaatccag ctgatcctca atacgccgct ctatacctac caggtcacga
     4801 aaaaataaga agtaggccct cgactgcagg accggggatg attcataaat cccgcttccg
     4861 gatgggggaa accaaaatga agtttatctt cattaaaaaa acatgttcag gccccttcgg
     4921 ttactgatcc tgttgaccgg attgcaaacc ctgtccgccc aggaaaagca acactttacc
     4981 aagtccgaca cccagcaggt tgaaaaactg ctcaaaagcg cctggtcggc cgcccggagt
     5041 gatccccatc tcagcctggc caccctgcac caggccgact ccttcatcaa ggccagccgg
     5101 accgattatt tttccagcgt gttgatttac tattatggcg tcgtgtataa aaacctgggt
     5161 gcctatgaca gcagcgaaat atattttgac cgttacattg aatacagtaa agctaaaaac
     5221 gacctccgga ggctggctcc cgtattcatg gccaaggcca atctgtattc cgaccagaac
     5281 cgctatgaga aaagcatgga cgcggtcacc cagtctttgc gttatttcag ggaattgaac
     5341 gacacggccg gcatcatcac cggtcagagc aagatgggat acctgctctc ggaaatcgga
     5401 cgtttcgacg atgcggaaaa atatcaccgt ttttccttgg acctatcgac tgcaatcagg
     5461 gacacggtat ccatcgccat tgccctgact aaccttggtc tcctgtttga aaagaatcag
     5521 ggatacgaca gcgccctggt ttatttcagg caatccgaag ccctgagcgt tcaactgcgc
     5581 gacacctatg ccctggcaga cgaccggtac aatattggaa acacccttat gaaacagaac
     5641 cggctttacg aggcggagcc ctacctggac cagtccgttt tgctggctca ggaaactggc
     5701 actccgggtc agatcatttc ggcccggctg ctgttggcca cgctccgcct ggcccagggc
     5761 catcgggagg ccggtatggc cttgatggaa ggggtgctga aggagtttgc agaagaaatc
     5821 agcctgaagg accgcagcga gacctaccgt tcgctctatg aagcctacaa ggaaaaagga
     5881 aatatttcca gggccttgca ttacctggaa tcctggacca gcattaagga ttccattttc
     5941 aaccaggacg tctcccggca gatgaaccaa ctcgaggtgc aataccagac cacccagaaa
     6001 gaccaggccc tgcaattgct gaattccaat catgaattgg cccaatccag gctcagggcg
     6061 gcccggtacc ggatttatgc cctgggttcg ggactggccg cagtcatcgt cctgctgttt
     6121 ttactctggc gtctctggaa aaaaacaagg ctccagaatg cgctgatcca aaaatcgctg
     6181 acggaaaaag aaaccctgct ccgggaaatc caccaccgtg taaaaaacaa cctccagttc
     6241 atttcttccc tgctcaacct gcaggcccgt catgaaaagg atggtcaaag ccggtcggtg
     6301 ctgcgggaag gacaaaaccg cgtgaaatcc atggcgttga tccaccagaa tctttaccag
     6361 gaacataatc ttacgggagt cgagattaaa tcctatctgg aaaacctgat caaaagcctg
     6421 tttcattctt acaacgtctc ccctgatctt atccgcctca agctcgatat cgaacccatc
     6481 cggctcgatg tggataccat ggtgcccctc ggcctgatca tcaatgaact ggtcagcaat
     6541 gccctgaaat atgcatttcc cgaaggccga gaggggtgga tcgaggtaag cctcagggaa
     6601 gaaggcgggc aattgttttt gcgggtttcc gacaacggca tcggactgaa ggacaaaaac
     6661 ccggagaaag acggccattc cttcggatac cgcctcatcc acgcttttca aacccaactg
     6721 aatgccagtc tggagctgaa gtccgatccg ggtaccaccg taaccatgcg tatacaggat
     6781 taccgcaaag catcctgaaa tccaaactac ctcagggggt aggaaaaggc gattgggccg
     6841 gtacgacctt tggcccattc accagtaaat ccaaaatcat gaaaatccaa actcttctat
     6901 taggcaccct gattacgttg tcggcctgca acaaagacga tgccccggat ttttccggct
     6961 cctaccaggc tttccggctc aggtcggaat gcaccgaaac aagcaagaat gtttccagaa
     7021 cctttgacgc ttccactaaa gagttgtgca ataccgtcac gggcgggcag gattgttttt
     7081 ccttgagcct tacgctgaat gcggatctga gttattcgct gacctccaaa accaaacaaa
     7141 tcagggccaa cggagggctg gtcacgacca aaccggctac cgacaacggg acttataccg
     7201 tgtccgggaa tgagttgacc ctttgcgtcg gcggagggag ttcgtgcatc actttaaaaa
     7261 ccacttcggt gacaggggaa ctcgactggc tgtccacaaa cagcagcggc tgtaattatt
     7321 tttatacctt taaaaagaac tagaaatacc ttgcctggcc agccgaccca acgtatccgt
     7381 agattaaaag gtcaataaaa gatgagccag atccgtatcc tgatcattga ggacgaaatc
     7441 atcatcgcgg aagacatccg ggatatcctg accgcattgg gatatgaggt cgccggcatt
     7501 tttaccaatg gggacaaagc cgtggattat ctgagttttc acagcccgga cctggtactg
     7561 tgtgatatca gcatcaaagg caacatggat ggcatccagg tggccgaaaa aatcaggtcc
     7621 aagaaaaaga tccctttcat tttcctgact tccctgtctg accggtctac cctggatagg
     7681 gcgaaaaagg ccctgcccta tggatacatt gtaaaaccct ttgatgaacg ggacctcctg
     7741 gccgggatcg aaaccggact ctacaaattc agccaggaac tggaacagct ttccatcacc
     7801 cgggaaaaga tcgacctgtt ggttacggat cccctgaccg accaggaata ttccattgta
     7861 aaagccatga tggaaggttt gaaaacggag gagatctgcg gcaaaatgtt tttttcggta
     7921 aataccttga aatatcacgt taaaaacata ctctccaaat tccaggcaga aaaccggggc
     7981 gaaatcatgc aaaaaatcat tgccggatgg atggttgcaa aataaaaccg tccccaacta
     8041 aagcaggata aaaccgatgg aagatcacga agcagaaaag gaaatcgacc tcaaggacaa
     8101 tctcctgatg gcctcctgga tccttgactg gcttcgccaa atggataccg acctgcatca
     8161 ggccgggctg gatgaccctg gcccggattg agcctatttc aggtttgcga ggtatttatt
     8221 ccagacttcg ttgccaacct tccggcccat attgaggcct tcgacattat cggcctggat
     8281 atggtatccc ccgagcacac gggatatccc ggcctggtcc gccgtattgg taaacgtcgg
     8341 aaatctcatc gtcaccgtat ccccgagccg gttggtttcc gtcaatgcgc cggccaccct
     8401 ttggacctca atgccgaagg tgtccgagcc tgtgtacaat ctcaatattt ccgaacaagc
     8461 cccgctgacc gtactgtgac cggaaacgta agcgggaaac ggagggcaga ggaaattgtc
     8521 cggggagtag ggccgccatt cagctccttt gatttttgtc cagccctttt cgggtcccgc
     8581 ccaggcatcg atggtttgat tcttaaagta ttcatggacc agcgcgtaag ggcgtgcata
     8641 gtcgtaatac atcttggttt cccagcaggc aataaaggca tccatggcag ccgcttccac
     8701 caggaaatac atgaccacat cctggtccag ggtatgctgg tcgcgcacgg aaacatcctg
     8761 tgcaaagatc agccagtgcc cggcctgttg gacggatttg gggccgtccc gcataaattc
     8821 aacaagagct ctttgttcat ccgtcaaccg ggcttgcatt tccaccactt cccgcacctc
     8881 gttcttcagt tgttcggagc cgagggccgg cggtgggggc gatctgaact ggcttgcgct
     8941 gtccaggatc agggttttga ccttgcccca gtgtggggta aggcagccgg gggcataggt
     9001 cccgagcacg ctgtccctga aatatttggg agcccagcgc atcaggtctt tggtatcatc
     9061 cgcagtattc agaggttgat agccggtata gtcctggtaa gtctggggtg cgtcgggcat
     9121 ctctccgtat tgattggaac cgtccatttt tcgggcctcg aacacggccc gggcagccag
     9181 ccttcctata ccagccgggc tgcttgggtc ctcgctggca tcggcgggat ccatacccag
     9241 ggaaatcatg tattcgttca ggcggatggt atccgaaaaa taaaattgtt tcaaggcttc
     9301 ataggccgcg taactgatgg tgatctcctt gtttttttct gtgaattcag aaaccggcct
     9361 ccgctccacc tgtttcagat agaccggttt ggcggtggaa tcataccggg accaggcatc
     9421 atagatggcc gtccaggtca ggcccagcat cctggaagtc accgtaggac ggggacggaa
     9481 tctttcggta tcgttggcgg tgagctccag ggatatcttc ccccattggt aggccaggtt
     9541 gtccgcaccc cttttcagcg gcatatcgac agttgtcccc ttgggcttgc attgaaggct
     9601 tgcaaaaagg aacaaaccgc agacaacaat cgcagttttg gttttcacag tttctaagtt
     9661 tctgaatgca atttaggatg attttaagag aaagaatgac ggcagcggga cgaattatca
     9721 cagagggtcc gcccgatttt aaattcttat gactttctaa gttatggatg aatggcccga
     9781 ctaattgggc ctggcctgaa ggaatcagcc catcgggccg tgagcggcgt gatcatttct
     9841 aaagtttact ttaaatatgt cgttcaaatt taaagtatac ttaaaaggcc aatctggctt
     9901 tcatcaaacc acagattctt cctgttcgga gacaggggaa ccggcagccc actaccttct
     9961 ccaatcaccc ttcatcaggg aagcaccctc actgtgaccc tgagcccttg atctgtatag
    10021 cccggctgtc cgccccctgc aaagatctca taattgcccg gcagatattc cttttcgccc
    10081 ttttcattga tttgggaaaa ggcatcgtcg cggagattca attgaacgac cttcgattcg
    10141 ccggctttga ggtggaccct ttggtaggcc ctgagtgaaa ggatgggctg gccctcgccg
    10201 gcatccggct ttttgacata gacctgcacc acttcatccc cgtccatgcc ccctttgttg
    10261 ctgactttca ggcttatcga aacgacttga ttttttgcaa ggacagtatc catctccagg
    10321 tccgaaaatt caaaccgggt atatgacaat ccatgtccga aaggatacag gggtctgcct
    10381 ttaaaatacc gataggtttg gctggtgatc tcatattgat caaaaggggg taggtcctcc
    10441 tcggagcggt agaaagtcac cggcagcttg ccgcccgggt tgtagtcgcc gaaaagcaca
    10501 tcggctatgg cagtacccgc ggcctggcct gggtaccagg cttccaggat ggcgggaagg
    10561 ttttcgtctt cccagttgac cgcaagggcg ctgccgttca ggagtaccag caccaccggt
    10621 ttgcccaggg cctgaataga acggatgagg tcctgctgga tccccggcaa agccagtttg
    10681 gtgcggtccc ctccccggaa gccgtcgatt ttgatatcca tttcttcgcc ttccatcctc
    10741 ggggtgatcc ccatgcagag caccacggca tcggcctcgc gggcaagggc cacggcttct
    10801 tgttttaggt tgggtttagg ggcggcccat acgagctgga cctgcgcatc ggcaaacgtt
    10861 tccgaagcat ccaccctgat tttataggac cttcctttct ccaggtgaat gggtaaggac
    10921 tttcgaagtc tgggatcccc gtattcattc ctgaaatgat aaacggtttt cgcgaccaca
    10981 ctgtcttcaa aatataattg cgtattgcag cttgtgatca ctcccaattg atagatgccg
    11041 ctggcttgag gccggagttc agcggtccac cggactccga aatcgtcatc gtccatatcg
    11101 gtacgcggcg cccggtcatt ccagttggca tccaggatgg ggtctgtggt tgttgcaagc
    11161 ggttcaccgg acaggtcctt attgccaaaa tattccacct tcaggccttt tgaagttcca
    11221 ttgaacaagg cttctcccgg gatggtataa aacataggca tgccttcagc gagctcacag
    11281 ccctgggcaa acagcacttt ggtattgggc aatttttcgc ggattccccg caacggtgtc
    11341 accgggtcag cgggaactcc attgtaattt cccagaagca tcagccactg gtcggcattg
    11401 ggcccgatga cggcaaccgt ttttaaatcc tttttcaagg gcagggtttg gccttcattt
    11461 ttcagaagga ccatggattt ccgagcggtt tccagggcca gttgccggtg cgcttcctga
    11521 tccacgaccg catacggtat ttgtgcccag cggacccggc tgctgtcgtc aaacatgccc
    11581 agtttcatcc gcgcaacaaa aagacgcttc agggcggtat ccaactcttt ttcggtgatc
    11641 aggttttggt ccaccgccgc cctgaggtgt ttaaaaaagc gtccgcattc caggtcggtg
    11701 cctgatttga tggccagcgc gaccgcttct tcgggggtcg ccgtgatttt atgatgcagg
    11761 gcgatgtcat cgatggcgtc gcagtccgag accacataac cgtcaaaatt ccaatctttc
    11821 cggaggatgc cctgaagcaa tccctggctg ccgcagcagg ctaccccgtt gagccggttg
    11881 taagcgcaca tgagggaata ggctttgcct tccctgattc ccatttcaaa ctggggcagg
    11941 taggtttcat acatatcctt gagattggcg cgggcatcga acgaatgtct ttccggttcc
    12001 ggtccgctgt gcacggcaaa atgttttacg gttgctatgg ttttgaaata aaccgggtca
    12061 tccccctgca gccctttgat gaattgcaca cccagggtgc cggtcagaaa cggatcctct
    12121 ccatacgttt cctgccccct gccccagcgc ggatcccgga agatattgat attgggagac
    12181 cagagggtca gcccctggta aataaacctt ttatttctcc tcacgaattc atggtgtttc
    12241 gcccgggttt catccgatat cacattggcc acgcgatgga tcagatcctc atcccaggtg
    12301 gctgccatac cgatggcctg gggaaacacc gtcgccaagc cagcgcgggc gatgccatgg
    12361 aggccttccg accaccagtt gtattgagga atgcccaagc tgtcgatggc cggggcctgg
    12421 ttcatcaatt gggcgatttt ttggtccagg ctcaggcggc tgaccaggtc ttccgccctt
    12481 ttctccatgg tcagatccgg attttgaaaa gggaag
//
LOCUS       DAOQXT010000124        12186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5693_length_12186_cov_5.475723, whole genome shotgun sequence.
ACCESSION   DAOQXT010000124 DAOQXT010000000
VERSION     DAOQXT010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12186
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5693_length_12186_cov_5.475723"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1200)
                     /gene="mltG"
                     /locus_tag="PKM27_16720"
     CDS             complement(169..1200)
                     /gene="mltG"
                     /locus_tag="PKM27_16720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNR08965.1"
                     /translation="MKRTVRNLLIVGILAGGLVAYYYYDQLFSPVLFDPLQNTDLKIR
                     TGSTFEDVVFQLQQNKQLKRVNGFKWLSRKMGYSDKSVKPGRYVLTPGMNNISLVRLL
                     RSGNQTPVKVVIYEGRTPDEILRKTEDDIETDTRSMLDTLYSEHFLQTIGLTRDHAMC
                     IFIPNTYEVYWNISPRNFLLRMKQESDRFWSRNNRKSLASKKGLSPEEAYTLASIVEK
                     ETNHGPEKPTIAGVYLNRLKNGIRLQADPTVVFAKKAFTQQRVLHADLSFESPYNTYL
                     HDGLPPGPISIASIESIDAVLNAENHDYLFFCAKPGYTGQHAFAATHAQHSRNARLFH
                     EWMNQQDIR"
     gene            complement(1201..2016)
                     /gene="panB"
                     /locus_tag="PKM27_16725"
     CDS             complement(1201..2016)
                     /gene="panB"
                     /locus_tag="PKM27_16725"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNR08966.1"
                     /translation="MSIHKEIKRITTQVLQEMKAEKQKIAMLTAYDFSMARILDASGM
                     DILLVGDSASNVMAGHETTLPITLDQMIYHAASVIRAVRRALVVVDLPFGSYQGNSRL
                     ALESAIRIMKEAGAHSVKLEGGQEIENSVRRILSAGIPVMGHLGLTPQSIYKFGTYTV
                     RATEEEEANRLKEDAMLLESLGCFALVLEKIPASLAKEVTGSLHIPTIGIGAGPHTDG
                     QVLVSHDMLGLNKGFSPRFLRRYANLYETILNASQQYIRDVKSSNFPNEQEQY"
     gene            2389..3183
                     /locus_tag="PKM27_16730"
     CDS             2389..3183
                     /locus_tag="PKM27_16730"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNR08967.1"
                     /translation="MQAYLDLLRKILAEGNEKTDRTGTGTRSLFGYQMRFDLSKGFPL
                     LTTKKLHVKSIIHELLWFLKGDTNIAYLKAHGVRIWDEWADPKGDLGPVYGRQWRSWP
                     SPDGRRIDQIQQVLDTIRQNPDSRRILVSAWNVAELDQMALCPCHCLFQFYVSEGRLS
                     CQLYQRSADVFLGVPFNIASYALLTHMVAHVSGLKAGEFIHTFGDVHLYNNHLDQAME
                     QLSRTPKALPELRINPQVKDLFAFRYEDFEIIDYHPDPAIKAPVAV"
     gene            3368..4789
                     /locus_tag="PKM27_16735"
     CDS             3368..4789
                     /locus_tag="PKM27_16735"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNR08968.1"
                     /translation="MIYKMFRFSFLLAVCTMVVPGLLPAQGNPDEGKTLFANNCAQCH
                     ARDMKTKATGPALAGFLDRWAAYPQEDLYAWIRNSQALISQGHPRANELWKEWGPVVM
                     TGFPNLTDAQIQSIEAYINGVADGTYGAVAKAPGAGALPVETKAKGLSWIWYALLGIL
                     LVMAAILGRLISGLNQLSAQQAGEPLPRQKTVWEWLTSKSMITTLIVLAVILAGYTTT
                     KNGIAFGRQQNYQPDQPIKFSHATHSGLNKIDCQFCHDGARRSKHAVIPAMNTCMNCH
                     KAIVKGSQYGTAEISKIFASVGFNPNTGQYIPDYENMAEKDIEVIFKKWIGDNHMTDK
                     ELKAMDPEGERLMNEQWRGIKNSLTNPTKPKIQGPVEWVRIHSLPDHVYFNHSQHVAV
                     GEVACQTCHGKVENMEVVRQYATLSMGWCVNCHRETEVKSFSSNDYYLGKYEKYHQAL
                     KDGSKKTVTVEDIGGLECQKCHY"
     gene            4832..5857
                     /locus_tag="PKM27_16740"
     CDS             4832..5857
                     /locus_tag="PKM27_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08969.1"
                     /translation="MKDKNAGIWIGNRDLNQDEAYIHQADEQSRIRPDETGGLQSNRR
                     DFLRIMGFSVGTAALAASCKTPVRRALPYLTKPDSLVPGIANYYASSYVDGGDYCGIL
                     VKTREGRPIKIEGNSLCPLTGGGTSARVQALVLSLYDTHRIQGPMVRGENNWESSSWE
                     NLDERVALRMPATSRVRILSNTILSPTTKKAISDFKVKFPNTEVVSYDPISSAAILMA
                     NEASFGRKIIPSYHFDQARTIVSINADFLGTWISPIEFSRQYAKGRKISEVSGAKMSR
                     HFQVESYMSLSGSNADHRIQIRPSEQGAAIAFLYNAIAVKTGAASVVSPPLAEKAASA
                     LSNVPIS"
     gene            5854..7992
                     /locus_tag="PKM27_16745"
     CDS             5854..7992
                     /locus_tag="PKM27_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNR08970.1"
                     /translation="MIADKGASLVVAGSNNRDEQILVNKINEILGNTGTTIDLNTPSY
                     QRQGNETDLVKLVADMDGGQVDAVIVIDHANPAYDYPAADAFATAFAKVNTRISMSGT
                     LNETANLCNFIAPNHHLLESWGDAEPKQGLLSLIQPAISPLFNTRQAELSLLKWATSD
                     HIDWKSDAPYYEYLKSNWVQNYLNGSSWDKALHDGVFKYQAPDLASAAPVVIDLAALK
                     ISQPTNAEQEITLFETVNMGAGQHANNPWLQEMPDPVTRCVWGNYLAVPVMFDGVNKF
                     KGAFDLENGDFVNLSVGNTTIETASIQQFGQLSGTLALALGYGRTVTGAAGKHIGTNA
                     FAFCQQADGLTQYYNTVGSSPVKSRKEENLACVQYHHTMGVQDIDRKTGQKINADEAA
                     TVFFTYFGLAKQGFQGSLVERSVIRRANLSELESFVSELQAERAEFQHLNEQTLYPDY
                     SDLYERGHQWHMHIDLNACTGCGACAVACMAENNVPVVGKREVSRHHEMTWLRVDRYY
                     YGDVANPNVVFQPLMCQHCHNAPCENVCPVNATNHNSEGINQMVYNRCVGTRYCANNC
                     PYKVRRFNWLDYMKADLWPINEPALVEGEDSPFMTDNLTRMVLNPDVTVRARGVIEKC
                     SFCTQRIQEGKLNAKKAGRMLEDGDVKTACQTACPTGAITFGDINHTASSVHQLANNP
                     LNFQVLEEVNTRPAVTYKSRIINRDESIEA"
     gene            8035..9468
                     /gene="nrfD"
                     /locus_tag="PKM27_16750"
     CDS             8035..9468
                     /gene="nrfD"
                     /locus_tag="PKM27_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNR08971.1"
                     /translation="MSVVVSPFREPLVRGGKTYHQITEDLCAATERAPSLAWVLAFIA
                     AGSALAFGLFCIVWTIWVGIGSWNLNRTIGWGWDITNFVWWIGIGHAGTLISAILLLF
                     RQKWRTGVNRAAEAMTIFAVICAALFPGIHVGRIWVVYFFFPYPNTRGPLWPNFNSPL
                     LWDLFAISTYFSVSLLFWYTGLVPDFATVRDRAKGFRKKLYNFLSFGWTGSAKHWQRW
                     EALSLVLAGISTPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVQTL
                     MLMTRKVLKLEDYITVNHIESMNKVILLTGTIVGVAYLTELFIAWYSGYVYEQFAFFN
                     RALGPYWWSYFGMMACNVISPQLFWIKKFRRSIYWTFLMSIFVNIGMWFERFVIIATT
                     LARDYLPSSWSLYSPTWVEIGIFIGTLGIFFTCYLIFVRLAPVVAIAEVKHILKAAGD
                     QYVDKNNAYAREAALHPPVQHETAGHH"
     gene            9493..10014
                     /locus_tag="PKM27_16755"
     CDS             9493..10014
                     /locus_tag="PKM27_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNR08972.1"
                     /translation="MSSHKEVIYGLYNDEEDLLKAVKQANAMHLPIMDVFSPFPVHGL
                     DPALHLAESRLHAGGFVYGLLGLLTGFLGMTWIFTKDWPIIFGGKPYWSVPAFIPITF
                     EMTVLFAAWGMTITFYIVCGLGPGITNKVLDERITDDKFCIAFNKASLGDTGKVNEFF
                     RSTGAAEVNTKVL"
     gene            10018..10782
                     /locus_tag="PKM27_16760"
     CDS             10018..10782
                     /locus_tag="PKM27_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNR08973.1"
                     /translation="MKKYLYLSLMVAGLSLILACSPAGGNRTGHEFMPDMVHPVGYEA
                     NFYSYYYFNRWGTKEEYKKFATPRLPVKGTIARGYAGMAASAAGITPNGSVPYYYGDT
                     EDERARAAREIIANPFPITEKGLARGKQLYDINCGICHGEKADGGGYLAREGGKYPVA
                     PANFKLDTFYNSTNGRFYHAIMYGKNMMGSYADKLSYEERWQVIHYIRSLQAKEKKLA
                     YDEQVNTFTTADIPGSKWPPAEPQPSMPQPETNPKH"
     gene            10800..>12186
                     /locus_tag="PKM27_16765"
     CDS             10800..>12186
                     /locus_tag="PKM27_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08974.1"
                     /translation="MADLHHTSSAQTSSQRFELTTTARTVLLSGVGLGVLSLLLVFFN
                     DDPFHTRFWTNILLNTLFFTGIALFALFKYSAGVVAFGGWFVGFKRIWEAYSLFLIVS
                     LIILLVLAGGLYLGYHHLYHWNDAATASSDRIISGKSGFLNKNFFTFFTIVALGIWIY
                     LARKLRSISLDEDVRGDASYSHHKRLRHYSSLALPLIGFLSAAFFWLWLMSLDAHWYS
                     TMFAWYSSASVFVAMLALTIGIILYVKSKGYLQEVTAEHLHDLGKYTFAFSIFWTYLW
                     FSQYMLIWYGNIGEETIYFRTRFDHYKVLFYANLVLNFLLPFLILMRNDTKRKTGTLV
                     LVAILVFFGHWIDFFQMVKPGALHTSHELIENRSSMGQLEQLEAKPMEVSQEEESHAG
                     AAADTGHTGAVAGQNMDTAHSASEQAHEASTEGHASTGNHGEDTHGGSGFVAGFTLPG
                     LLDLGVMIGFLA"
BASE COUNT         2929 a         3142 c         3166 g         2949 t
ORIGIN      
        1 gtttttaagt gtttttcaac cttaaaaacg gtcaacctct gtcaggcatt gacaggtaag
       61 aggtaagagg taggaggcaa gaggtaagag gtaagaggta ggaagtgcgc acctcgaacc
      121 tcgcacctcg aacctctaac ctcttacctc taacctctta cctctaaact acctgatatc
      181 ctgctgattc atccattcat gaaacaacct ggcattgcgg ctgtgctggg catgggtggc
      241 cgcaaaggca tgttgtccgg tgtaccctgg tttggcgcag aaaaaaagat aatcatggtt
      301 ttccgcgttg aggaccgcgt cgatgctttc tatcgaagcg atggagatgg gccctggcgg
      361 aaggccatca tggagataag tattatatgg ggactcaaaa ctgaggtcgg catgtaaaac
      421 cctctgttgg gtaaatgctt ttttggcaaa gacgaccgtg gggtcggctt gcaggcggat
      481 cccgtttttc aggcggttga ggtaaactcc cgctatcgtg ggtttttccg gtccgtgatt
      541 ggtctccttt tccacgatgc tggccagggt gtaggcttcc tcagggctca gtcctttttt
      601 tgaagccagg cttttgcggt tgttgcggga ccagaacctg tccgattcct gtttcatgcg
      661 caataggaaa ttgcgggggc tgatattcca gtaaacttcg taggtattgg gaataaaaat
      721 acacatggcg tgatcgcggg tgagccctat ggtctggagg aaatgttcac tgtacagggt
      781 gtccagcatg gaccgggtat cggtttcgat gtcatcctcc gttttgcgaa gaatctcgtc
      841 cggggtgcgt ccttcataga tgaccacttt taccggggtc tggtttccgc ttcgaaggag
      901 ccggaccagg ctgatgttat tcatgcctgg ggtcaggaca tatctgcccg gtttgacgga
      961 tttatcggag taacccattt tacgcgacaa ccatttaaag ccgtttaccc tttttaactg
     1021 cttgttttgc tggagttgga agacgacatc ctcaaaagtg gatcccgtcc tgatcttcag
     1081 gtcggtattt tgcaaggggt caaacaaaac cggggaaaac aactggtcat aataataata
     1141 ggcgacaagc cctccggcca ggattccaac tataagaagg ttccttactg tacgtttcat
     1201 tcaatattgt tcttgctcgt tggggaagtt ggaagatttt acgtcacgga tatattgctg
     1261 gctggcgttc agaatggttt catacaggtt ggcatagcgc cggaggaacc ggggcgaaaa
     1321 tcctttattc aggcccagca tgtcgtggga aaccaggacc tggccatcgg tgtgcggacc
     1381 ggcgccgatc ccaatggtgg ggatatgaag ggagccggtg acttcctttg ccagggaagc
     1441 ggggatcttc tccaggacga gggcaaaaca gcccagggat tccaggagca tggcatcctc
     1501 tttcagccgg ttggcctcct cttcttctgt ggccctgacg gtataggtcc cgaatttata
     1561 aatggattgg ggagtgaggc caaggtgccc catcaccggt atcccggccg acaggattcg
     1621 cctgacgctg ttttcgattt cctgcccgcc ttccagtttt acggaatggg cgcctgcttc
     1681 cttcatgatc cggatggcgc tctcgagggc gaggcgggaa ttaccctggt aggatccgaa
     1741 tggaagatcc accaccacca gggcccggcg gaccgcccgg atcaccgagg cggcatgata
     1801 aatcatctgg tccagggtga tgggcagggt ggtttcatgg cctgccatca cgttggaagc
     1861 cgaatcaccc accaaaagga tatccatgcc ggaggcgtcc aggatcctgg ccatggaaaa
     1921 atcataggcc gtaagcatgg ctattttttg cttttcagcc ttcatttcct gaaggacctg
     1981 ggtggtgatg cgtttgattt ctttgtgaat ggacatggcg cctgttgagg gggtaaagtt
     2041 aaggcaccgc taaaaacccg ttcacggttt aatcggtttt taaattaaaa aacctgtttg
     2101 gccctgttat cccgattata ttgcgttgaa tatcagtcat cccgtcctgg acgggacccc
     2161 tccaggttct ttcgatttgc ctggtcttaa tcccgaccct gatcaggaca aaataataaa
     2221 tacttgaact cattcaggct tttcagaaat gcccttaatg cctccggcga tttcgaggta
     2281 aggactgttc tgagatttac cggagaaggt ttatctggtc ggaaaacccg ccaatccccg
     2341 gcatcatatt aactttattg ataatccaac atttcctact tttggcccat gcaagcatac
     2401 cttgacttac tccggaaaat cctggctgag ggcaatgaga aaacagaccg gaccggaacc
     2461 ggtacccgaa gcttgttcgg ataccagatg cggtttgatt taagtaaagg gtttcccttg
     2521 ctgacgacta aaaaactgca tgtcaaatcc ataatacatg aactcctttg gtttctgaag
     2581 ggggatacca atatcgccta cctgaaggcc catggggtca ggatctggga tgaatgggcc
     2641 gatcccaaag gggaccttgg gccggtctat ggccggcaat ggaggagttg gccctccccg
     2701 gacgggcgcc ggatcgacca gatccagcaa gtcctggata cgatccgcca gaacccggac
     2761 tccaggagaa tcctggtcag cgcctggaac gtggcggaac tggaccagat ggccctttgc
     2821 ccctgtcatt gccttttcca gttttatgtc agtgagggca ggctgtcctg ccagttgtac
     2881 cagcgttcgg cggacgtatt cctcggggtg ccttttaata ttgcctccta tgccctgctc
     2941 acccatatgg ttgcccacgt cagcggcctt aaggcgggtg agtttatcca tacttttggg
     3001 gatgtccatc tgtacaacaa ccacctggat caggccatgg aacagctgag ccggacccca
     3061 aaggccttgc ccgaactacg gatcaatccc caggtgaagg atctttttgc cttccggtac
     3121 gaggattttg agattattga ttaccatccg gatccggcga ttaaagctcc ggttgcggtg
     3181 taaatcaggg gattaagatg cagcaaaaca aaattaatag gtcaagttat gataaattca
     3241 attggtttat ttagacaata tctaaatagg aaaattacga gatgagaact gttaaatgaa
     3301 ttaaccattg tctatttttg cgacgctgat ttcagtaaat atggatcatt ttttaatata
     3361 agttttcatg atatataaaa tgttcaggtt tagctttttg ttagctgttt gcaccatggt
     3421 cgttccgggg cttcttccgg cgcaaggcaa tcccgatgaa ggtaaaaccc tgtttgcgaa
     3481 caattgcgcc caatgtcatg ccagggacat gaaaacgaaa gccacggggc ctgccctggc
     3541 aggatttttg gaccgctggg cggcttatcc gcaggaggat ctgtatgcct ggatcaggaa
     3601 ttcccaggcg ttgatttccc agggccatcc cagagctaac gagctgtgga aagagtgggg
     3661 gcctgtggta atgaccggtt ttcccaacct caccgacgcc cagattcaat ccatagaagc
     3721 ttatatcaat ggggtggccg atggtaccta tggcgccgtt gccaaggctc ccggagctgg
     3781 agccctgcct gtggaaacca aggcaaaagg cctcagctgg atctggtatg cgctgctggg
     3841 aatcttactc gtgatggcag cgatccttgg caggctgatc agcggtctga atcaattaag
     3901 cgcccaacag gccggagaac cccttcccag gcaaaaaacc gtctgggaat ggcttaccag
     3961 caaatccatg atcactaccc tgatcgtcct ggccgtgatt ctggccggat atacgaccac
     4021 caaaaacggg atcgcttttg gcaggcaaca aaactaccag ccggaccaac ccatcaaatt
     4081 ttcacatgcc acccattccg ggctcaataa aattgactgc cagttttgcc acgatggagc
     4141 cagaagaagc aaacacgcgg tcattccggc catgaatacc tgtatgaact gtcacaaagc
     4201 cattgtcaag ggttcccagt acggaactgc ggaaatcagc aaaatatttg cctccgtcgg
     4261 cttcaatccc aataccggtc aatacatccc cgattatgaa aacatggccg aaaaagacat
     4321 cgaggtgatc tttaagaaat ggattgggga caaccacatg acggataagg aactcaaagc
     4381 catggatccc gaaggggaaa gactgatgaa tgaacagtgg cgggggatca aaaacagcct
     4441 caccaatccc accaaaccca aaatccaggg ccccgtcgaa tgggtacgga tccacagtct
     4501 tcccgaccat gtttacttta accattccca gcacgtagcc gttggggagg tcgcctgcca
     4561 aacctgccac gggaaggtgg agaatatgga ggtggtcagg caatacgcaa ccctctccat
     4621 gggctggtgc gtaaattgcc accgggagac cgaggtgaaa agtttcagct ccaacgacta
     4681 ttacctgggt aagtatgaaa aataccacca ggcgctcaaa gacggatcaa agaaaaccgt
     4741 cacggtggaa gatatcggtg gcctggagtg ccaaaaatgc cattattaaa caagacgcag
     4801 cattcacata tataatacca atcatagcag tatgaaagat aaaaatgccg gaatttggat
     4861 agggaacagg gatctgaacc aggacgaggc ctatatccat caagcagacg aacaatcccg
     4921 tatccggccc gatgagaccg gcggccttca atccaaccgg agggactttc tgcgaatcat
     4981 gggattcagt gtaggcacgg ccgcccttgc cgcaagttgc aagacgccgg ttcgcagggc
     5041 tctgccttat ctgaccaaac ccgactccct ggtccccgga atcgccaact attatgcctc
     5101 ttcctatgtg gatgggggcg attattgcgg catcctggtt aaaacaaggg aaggccgacc
     5161 catcaaaatt gagggaaaca gtctttgccc gcttaccgga ggcggaacct ctgcgagggt
     5221 gcaggccctg gtcctgtccc tttacgacac gcaccggatt cagggcccga tggtcagggg
     5281 tgaaaacaat tgggagtcct cctcctggga gaacctggat gagcgtgtgg cgctccggat
     5341 gccggccacc tcaagggtca ggatcctatc caatacgata ttatcgccga ccaccaaaaa
     5401 ggccatctcg gacttcaagg tgaaatttcc gaataccgaa gtggtgagct atgatcccat
     5461 ttccagtgca gccatcctta tggccaatga agccagtttt ggccggaaaa tcattccttc
     5521 ctatcatttt gaccaggcca ggacgatcgt aagcattaat gcggatttcc tgggaacctg
     5581 gatttctccg atcgaattct ccaggcagta tgccaaaggc aggaagatca gtgaggtgag
     5641 cggagccaaa atgtcacggc acttccaggt ggagagttat atgtctctgt ccgggtccaa
     5701 tgcggatcac cggatccaga tccgcccgtc cgaacaaggg gctgccatag ctttccttta
     5761 caacgccatc gccgttaaaa ccggggctgc ttctgtagtc agcccgcccc tggcggaaaa
     5821 agcggcttcc gcactgtcca atgtgccgat cagttgattg ccgacaaagg ggccagcctg
     5881 gtggttgccg gttcaaacaa ccgggatgag cagatcctgg tgaacaagat caatgaaatc
     5941 ctcgggaata ccggaaccac cattgacctg aataccccat cttaccaaag gcagggcaac
     6001 gaaacggacc tcgtcaaact ggtggctgat atggacggcg gacaagtgga tgcagtaatc
     6061 gtgatcgacc acgccaaccc ggcctatgat tacccggcag cggatgcctt tgccaccgct
     6121 ttcgcgaagg tgaatacgcg catatccatg tcgggcaccc ttaatgaaac agccaacctc
     6181 tgtaatttta tagcccccaa ccatcacctc ctcgaatcct ggggtgatgc cgaacctaaa
     6241 caaggccttc tcagccttat ccaaccggcc atatccccct tgttcaatac ccggcaggct
     6301 gaactgagtt tgctaaaatg ggccacatca gatcatatag actggaagtc cgatgctcct
     6361 tattatgaat atcttaagtc aaattgggta caaaattacc tgaatggatc ttcctgggac
     6421 aaggccctgc atgacggggt cttcaagtac caggctcccg acctggcctc tgcggccccg
     6481 gttgtcatcg atctggctgc cctgaagatc agccagccca ccaatgccga acaggaaatt
     6541 acactttttg aaaccgtaaa tatgggtgcg gggcagcatg ccaacaatcc ctggctccag
     6601 gaaatgcctg acccggtgac ccgctgcgtg tggggaaatt acctggccgt ccccgtgatg
     6661 tttgacgggg tcaataaatt caaaggggcc tttgaccttg aaaacgggga ctttgtgaac
     6721 ctgagcgtcg gaaatacaac catcgaaacc gcctccattc aacaatttgg gcagctttcc
     6781 ggcaccctgg ccctcgctct tggatacgga aggaccgtta ccggcgccgc aggaaagcac
     6841 attggtacca atgcctttgc tttttgccag caggccgatg gactcaccca atattataat
     6901 acggtcggtt ccagccctgt gaaaagccga aaggaagaaa accttgcctg tgtgcagtac
     6961 caccacacca tgggggttca ggacatcgat cggaaaacag gccagaagat caacgccgat
     7021 gaagcggcaa ccgttttctt cacttatttt ggtttggcca aacagggctt ccagggatcg
     7081 ctggttgaac gatcggtgat ccgccgggcc aatctcagtg agctggaatc ctttgtatcg
     7141 gaactgcagg cggaaagagc agaattccag catctcaatg aacaaaccct ttatcccgat
     7201 tattccgatc tctacgagcg gggccatcaa tggcatatgc acattgacct caacgcctgc
     7261 accggttgcg gggcttgcgc cgtggcctgc atggccgaaa acaacgtgcc cgtggtgggc
     7321 aaacgggagg taagccggca tcacgaaatg acctggctga gggtggaccg ctattattac
     7381 ggggatgtag ccaatccgaa cgtggtcttc cagcccctga tgtgccagca ttgccacaat
     7441 gcgccttgtg agaacgtatg ccctgtgaat gcgaccaacc acaacagcga aggcatcaac
     7501 cagatggttt acaaccgttg tgtcggaacc cgatattgcg ccaacaactg tccttataaa
     7561 gtaaggcgtt tcaactggct cgattatatg aaagccgacc tttggccgat taacgaaccg
     7621 gctctggtag aaggagaaga ttctcccttc atgacggaca acctgacccg gatggttctc
     7681 aacccggatg tgaccgttcg tgcaaggggc gtgatcgaaa agtgcagttt ctgcacgcag
     7741 cgtatccagg aaggcaaact caatgcgaaa aaagcgggaa ggatgctgga agacggcgat
     7801 gtaaaaacgg cctgccagac cgcctgcccc acgggcgcca ttactttcgg agatatcaat
     7861 cataccgctt cgtccgtgca ccagctggcc aacaatcccc tgaatttcca ggtactggaa
     7921 gaagtcaaca ccaggcctgc agtgacctac aaatccagga tcataaaccg ggacgaaagc
     7981 atagaggcat gatcaaactg aacttattat taagcagcat ataaagcaat agatatgagc
     8041 gttgtagttt ctccatttcg ggaaccattg gtacgggggg gtaagaccta tcaccagatc
     8101 acggaagatc tgtgcgcggc caccgagcgg gctcccagcc tggcctgggt cttggctttc
     8161 atagccgccg ggtcggcctt agcctttggc ttgttctgta tcgtatggac catatgggtc
     8221 ggtataggaa gctggaacct caaccggacg atcgggtggg gatgggacat taccaacttt
     8281 gtttggtgga tcggtatcgg gcatgccggt accctgattt cagccatcct tcttcttttc
     8341 aggcagaaat ggcgcaccgg ggtcaaccgc gccgcggaag ccatgaccat tttcgccgtg
     8401 atttgcgcag ccctttttcc cggcattcac gtcggccgga tctgggtggt ctattttttc
     8461 ttcccttatc ccaatacccg cggcccgctg tggcccaatt tcaattcacc cctgctttgg
     8521 gaccttttcg ccatatcgac ttatttttcc gtttcccttt tgttttggta taccggcctg
     8581 gtgccggatt ttgccacggt ccgcgaccgg gccaaaggat tcaggaagaa gctttataat
     8641 ttcctttcct tcggatggac cggctcggcg aaacactggc agcggtggga agccctttcg
     8701 ctggttctcg ccggaatatc caccccgctg gtgctttccg tacatactat agtgagcttt
     8761 gactttgcca cctcggtgat ccccggctgg catacgacca ttttcccgcc ctatttcgtg
     8821 gccggggcca tcttctccgg ttttgccatg gtgcagacgc tgatgctgat gacccggaaa
     8881 gtgctgaaac tggaagacta catcaccgtt aatcacatcg aatccatgaa taaggtgatc
     8941 ctgctgaccg gtacgatcgt cggcgtggct taccttacgg aactcttcat agcctggtat
     9001 tccggttatg tgtacgaaca attcgcattc tttaaccggg ccctgggacc ctactggtgg
     9061 tcctatttcg gcatgatggc ctgcaacgtg atttcgccgc aattattctg gatcaagaaa
     9121 ttcagaagga gtatttactg gaccttcctc atgtccatat tcgtcaatat cgggatgtgg
     9181 tttgagcgct ttgtgatcat tgccaccacc ctggcaaggg attacctgcc ctcgagctgg
     9241 agcctttatt ccccgacctg ggtggagatc ggaatattca ttggtacgct cggaattttc
     9301 tttacctgtt acctgatctt tgtacggctc gccccggtgg tggccattgc ggaagtgaaa
     9361 cacattctga aagcagccgg cgaccagtat gtggataaaa acaatgccta tgcgcgggaa
     9421 gcggccctgc atccgccggt acagcatgaa accgccggtc accattaatt gaaaaataag
     9481 aaaccgataa acatgtcatc acataaagaa gtcatttacg gactgtacaa cgacgaagag
     9541 gatttgctca aagccgtgaa gcaggccaat gccatgcacc tgcccatcat ggatgtgttc
     9601 agcccttttc cggtacatgg tttggacccg gccctgcatc tggctgaatc caggttgcac
     9661 gccggaggat ttgtatacgg cctgctgggt ttgctgaccg gtttcctggg catgacctgg
     9721 atctttacca aggactggcc gatcatcttt ggcggaaaac cttattggtc cgttccggcc
     9781 ttcattccga tcacttttga aatgacggtc ctgtttgccg cctgggggat gaccatcacc
     9841 ttttatattg tttgcggtct gggacctggg atcaccaaca aagtgctgga tgagcggatc
     9901 accgatgaca aattttgtat tgcattcaac aaagcctcgc tcggcgacac cggtaaagtc
     9961 aacgaatttt tccgttcgac aggagccgct gaggtaaata ctaaagtgtt gtaaattatg
    10021 aagaagtatc tgtacctatc cctgatggtc gccgggctga gtttgatctt agcctgttct
    10081 ccggccggag gcaaccggac cggacacgaa tttatgcccg acatggttca cccggtgggt
    10141 tatgaggcta atttttactc ctattattat ttcaaccgtt ggggcacgaa ggaagaatat
    10201 aagaaattcg ctacaccccg gctgccggtc aaaggaacca ttgcccgggg atatgccggc
    10261 atggccgcct ccgcagccgg catcacgccg aatggttccg taccttatta ttacggggat
    10321 accgaagatg aaagggcgcg ggcggcgcgt gaaattattg ccaatccttt cccgatcaca
    10381 gagaaagggc ttgcccgggg caaacagctt tatgatatca attgcggcat atgtcatggt
    10441 gaaaaagccg acggcggcgg ttacttggca cgcgaaggag gaaaatatcc ggtagctccg
    10501 gcaaatttca agttggacac tttttacaat tccaccaacg ggcggtttta tcatgcgatc
    10561 atgtatggta aaaatatgat gggcagctat gccgataaac tgtcttatga agaaagatgg
    10621 caggtgatcc attatatccg ttccctccag gcaaaagaaa agaaactggc ctacgacgaa
    10681 caggtgaata cctttactac cgcggatatc cccgggagca aatggccccc cgcagaacca
    10741 cagccctcaa tgccccaacc tgaaaccaat ccaaaacatt gatcttataa cgaccagaca
    10801 tggcagatct tcatcataca tcgagtgcgc agacatccag ccagcggttt gagctgacta
    10861 ccaccgcacg gaccgtttta ttatccggag tgggactggg cgtcctttct ttgttgttgg
    10921 tttttttcaa tgacgacccc tttcacacgc ggttctggac caatattttg ctgaatacgc
    10981 tgttctttac cggaattgcg ttattcgcgc ttttcaaata ttccgccggg gtggtcgctt
    11041 tcggagggtg gtttgtaggg tttaaacgga tttgggaggc ttattccctg tttctgattg
    11101 tttccctgat tatcctcctc gtcctcgccg ggggcttata cctgggttat catcatcttt
    11161 accattggaa tgacgccgcg acggcgtcct cagaccggat catcagtggc aaatcggggt
    11221 ttttgaataa gaatttcttc actttcttta cgatcgtcgc cctggggatc tggatatacc
    11281 tggccagaaa attaagatcg atttcactgg atgaagatgt aaggggcgat gcctcgtatt
    11341 cccaccacaa acggttgagg cattattcgt ccctggcgct tcccctcatt ggatttttga
    11401 gtgcggcttt tttctggctt tggctgatgt ccctggatgc gcattggtac agcaccatgt
    11461 ttgcctggta tagttctgcc tccgtctttg tggctatgct ggccctgacc attggcatta
    11521 ttctttatgt caaatccaaa ggctacctcc aggaagtgac tgccgagcat ctgcacgacc
    11581 tgggcaaata cactttcgcc ttcagcattt tctggaccta cctctggttc tcccaataca
    11641 tgctgatctg gtatggaaac attggggaag agacgattta tttcaggacc cgctttgacc
    11701 actacaaggt cctgttctat gccaacctcg tactgaattt cctcctgcct ttcctgatcc
    11761 tgatgcggaa cgataccaag aggaaaaccg gcacccttgt tttggtggcc atcctggtat
    11821 ttttcggtca ctggattgac tttttccaga tggtaaaacc cggcgctctc catacttccc
    11881 atgaactgat cgaaaaccgg agcagtatgg gtcaactgga gcagctggag gctaaaccca
    11941 tggaggtgag ccaggaagaa gaaagccatg ccggcgcggc cgctgatacc ggtcacaccg
    12001 gtgcggtagc gggacaaaac atggataccg cccattccgc ctccgaacag gctcatgagg
    12061 ccagcacgga aggtcatgca tcgaccggaa accacgggga agacacacac ggcggatcgg
    12121 gatttgttgc gggatttaca cttcccggcc tgctggatct tggggtgatg attggattcc
    12181 tggcgg
//
LOCUS       DAOQXT010000125        11883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5869_length_11883_cov_5.316030, whole genome shotgun sequence.
ACCESSION   DAOQXT010000125 DAOQXT010000000
VERSION     DAOQXT010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5869_length_11883_cov_5.316030"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKM27_16770"
     CDS             complement(<1..693)
                     /locus_tag="PKM27_16770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR08975.1"
                     /translation="MKHIVILGTAYPFRGGLAAFNERLAEELIRMGHRVDLVTFTVQY
                     PSFLFPGKSQMSEDPSPPLTIYRWLHAFNPFNWILTGLKLRRLKPDLIICKFWLPLMG
                     PALSSVIRIAKTKNTLAISILDNVVPHEKRPGDKLFTRYFLACIDRFIYMSEQVGRDL
                     DDFKTGKPSRLIPHPIYDNYGQAVSRAEAVGHLRLDPDCQYVLFFGFIREYKGLDLLL
                     DAIALLPPCLDKL"
     gene            complement(690..1631)
                     /locus_tag="PKM27_16775"
     CDS             complement(690..1631)
                     /locus_tag="PKM27_16775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR08976.1"
                     /translation="MDISVVIPLYNEAESLPELEAWISRIMRENNFSYEILFVDDGST
                     DRSWEVIEKLAAFNENIRAFRFRRNYGKAAALNTGFHATRGHYVVTMDADLQDSPDEI
                     PGLVEKIRSEKLDLVSGWKKKRYDNALTKNLPSKLYNWTTSRMSGIRLHDMNCGLKIY
                     RQEVVKNIEVDGEMHRYIPVIAKWAGFAKIGEKVVAHQARKYGTTKFGMERFVNGFLD
                     LATIMFVGKFGKRPMHLFGTFGLLIFFIGFFILTYLTIGKFFFDFISIANRPIFYLGI
                     LFLIVGLQLFMSGFIAELVTRNAAVKHHYQIEKQIRA"
     gene            complement(1761..2294)
                     /locus_tag="PKM27_16780"
     CDS             complement(1761..2294)
                     /locus_tag="PKM27_16780"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNR08977.1"
                     /translation="MQETKPGPVILRFGLTGGLLSALIFLIFTVTGLSSPDKPTSGYV
                     SGFLTFAILVTLVVLAIRRQRDEVQGGYINLGQCILVGLGVVLLSTLISSLVSLVYTQ
                     FIDPGFTERMMAQIEDAWEAQGMSEEQIEAARSWTSFMKSPILTIAYNLVCFGIGGLI
                     LALITGLIMKRERPEIS"
     gene            complement(2332..3591)
                     /locus_tag="PKM27_16785"
     CDS             complement(2332..3591)
                     /locus_tag="PKM27_16785"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF024294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNR08978.1"
                     /translation="MEIILEQAKLIHPGHPGHGSRLNIRIRDGKIVSLKAGKDKTAKT
                     IKGKNLHVSIGWLDLGASVGEPGYEHRETVHSLTLAARDGGYTGVAVMPNTKPPLHSK
                     AEIEFITRKSHAADIDIYPIGAISKGCQGKELAEMIDMHTSGAIAFSDGLFPVEDNGV
                     MLRAMRYVNHFNGLIINHPEDQATAHLGQMHEGIVSQQLGLKGIPSIAESLMLQRDLY
                     LAAYADCRYLAHLVSTEESVKQFKAARKILPSAFSSVSYLNLLLEDASLLDFNSNFKQ
                     SPPLRTARDRKALIQGVKQKVIDIVCSNHRPLETEKKDLEFAYADPGTVGLQTAYAAL
                     ASEQVLEPEEWVLAVAFHSRRALKLALPAMELGAEANLTVFDPEAEWEFGPERVKSLS
                     KNTPFAGKRFKGRVLATVHKGIAYLNN"
     gene            complement(3606..5639)
                     /locus_tag="PKM27_16790"
     CDS             complement(3606..5639)
                     /locus_tag="PKM27_16790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNR08979.1"
                     /translation="MQFFHPWFLAALATLAIPVIIHLFYFRRYKKVYFPSVRFLKEVK
                     DETSARSKLKNLLVLAMRLLALAFLVMAFAQPFVRTSGVEKYSKNLVGLFVDNSFSMN
                     ALTEDIPLYSKAKQKARHIVEAYTGEDRFVMLTHEFQGKQLRLVHQDEALAMIDEIQP
                     TPHVHNLKDAQERIQSILRKETGNEHIYILSDFQRSITDLPVSADTLSKIHLLPLQSV
                     QEANLAIDSAWFDSPVQLLGQASLLKFRIRNYGNQPVEDARVSLVQNNQSKPLGTLRI
                     EPGASVVDTARIVVNQPGWQEVQVEITDFPVQFDDKIYLTFNVNARIDLTVISTDRIH
                     PDLLTALQSLPSANLRVQSLNNINYSTLAQNSLIILDGLREISSGLSNELQGALRQGS
                     NVLLFPGEAANLASYSGFVQALGGGEFQSFEKNVYPVTSINTEEFTFREVYNRVSSNM
                     RLPVVQGRYLKSRGGRLGEETIMGFADGRPFLSKLSAEGGTLFICSTPLAGEWNDLSR
                     NPEIFIPMLFRMAVHKKQADQISRFIGRDEIIETASTPLSAEEVVHLSKGGPAGQDYI
                     PYQRMAGSKVILDLKGSIQDAGIYQMTLRDTLVGKVAYNYNRKESDLRTFTLPELEEA
                     YGSAYEIVENAGGTDFESWIKNKYQGKTFWRWFVAATLLFLLFESLLLRFWKT"
     gene            complement(5734..6024)
                     /locus_tag="PKM27_16795"
     CDS             complement(5734..6024)
                     /locus_tag="PKM27_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08980.1"
                     /translation="MNTKSGLVVLVLFLFLVSCARSTNNLEPCISGHIYGFWGGLWHG
                     LIAPFDLLASLFDKEVYMYAPNNNGGWYALGFLFGSGGWGLLASKSTGKKRR"
     gene            6083..7105
                     /locus_tag="PKM27_16800"
     CDS             6083..7105
                     /locus_tag="PKM27_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNR08981.1"
                     /translation="MQKDIFKEQYAGINGYTFEKKENGSMNFVDYYQILGVSKSATTE
                     EIRKAYRKLARKYHPDMNQGNKEAERKFKEVNEANTVLSDPEKRKKYDTYGKDWEQAD
                     AFEQARRNQGGFGRGNGRSRQTRYTMDGEDVDPGMFSDFFQSMFFDQGDLFGSGSFRT
                     SGGTRQRSAPRGADYQSTLQLNLEDTLHEQSQVLEVNGKKIRIKIPAGVEHGQTIKVK
                     GQGGQHTPGGPVGDLFLSFEILPHPRFKTEGRNLLATHELDLYTAVLGGETDIRTLTG
                     NVKVKVKAGTQPGSKIRLKGLGLPYYKSDQKGDLILTYQVTIPQSLSQKEEELFRKLR
                     EIQSRT"
     gene            7139..7663
                     /locus_tag="PKM27_16805"
     CDS             7139..7663
                     /locus_tag="PKM27_16805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNR08982.1"
                     /translation="MTSKSYHGVEKKEPVIKEAMLSEYLGFPHAASLLFARLFEFIGI
                     PKPKGKNPLESDLIPLIRKGLVKKHLDKLMDTTGLDLASMAGILHVSSRTLHRYTEDS
                     VLNPDISERLLEIARLYARGQEVFGDLDTFKSWIQEPVPALGFKKPLEFLDTSIGIRL
                     LEAELGRIEHGVFS"
     gene            7663..8118
                     /locus_tag="PKM27_16810"
     CDS             7663..8118
                     /locus_tag="PKM27_16810"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNR08983.1"
                     /translation="MEVFRCSKCRYIRDLTGEGAYLYGGRWNSKGVRLLYTSSSAALA
                     LLETLVHTSRLLPAMDYCMLRLRIETDSMDTLPPSAMPDGWSDNPAPDELKRIGDRFV
                     KEGKYLMLRVPSAVLPMEWNYLINPGHDLFGKGKVDEVVEIRVDGRLIS"
     gene            complement(8442..9731)
                     /locus_tag="PKM27_16815"
     CDS             complement(8442..9731)
                     /locus_tag="PKM27_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR08984.1"
                     /translation="MKTRRQFLQKAGLLSGASLLAPLAGKAMQEKLDYAFRSRAGQSA
                     DALATDEDFWAMIQQAFTVNSNLINLNNGGVAPAPKSVQDAVERYARLSNEAPSYYMW
                     RILDQGREPLRKKLAALAGVSPEELALHRNASEALETAIFGLRLRQGDEVILCKQDYP
                     NMINAWKQREKRDGIKLVWLNFEFPMENKEAIINRYIGAITPATRVIHLTHIINWMGQ
                     IMPAKEIVREAHNRNVEVVLDAAHSFGHIQYDVADIGCDYFGTSLHKWISAPIGTGLM
                     IVKKEKIKNLYPLFAAGDPESEDIRKFENLGTRPFHIEQATGQAIQFHQMIGGERKQK
                     RLHYLKNYWASKANAIPGVRVATSLDPAYGCAIGLLQVEGKTPAQVDSFLFEKYKIHT
                     TGINWENIVGVRITPNVYTLPADLDVLVEGIEKCRKA"
     gene            complement(10341..11066)
                     /gene="istB"
                     /locus_tag="PKM27_16820"
     CDS             complement(10341..11066)
                     /gene="istB"
                     /locus_tag="PKM27_16820"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNR08985.1"
                     /translation="MNHQKTLELMRDMRLHGMYDAMQGLVQGKKLGSINADQLLAILL
                     QQEWDERNNRKIHRLTRSARFRYTASLQEIKADAKRNLSGEQLALISTCDWVNKAENL
                     MITGPTGVGKSHLASAIGHQCCQMGLKVAYHNTQKLYQALRLARLDGTHRRQMQLLAR
                     ADLLILDDFGLQRPDELARLDLLEMVEDRHGRRSMIIASQLPISIWYEIIAEPTIADA
                     ILDRIVSNSHRIELTGESLRKRK"
     gene            complement(11089..>11883)
                     /locus_tag="PKM27_16825"
     CDS             complement(11089..>11883)
                     /locus_tag="PKM27_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08986.1"
                     /translation="PKDKAKVERAAARSYLALFAPLRKQTFYSVQELNTALLEQSTLL
                     NQRMMQQYQCSREVLLERDERPALKALPNERYQIKQHLILTVQQNGHVFLSKSKRYFS
                     APYRYIGLKVHVIITPSLVRIYHQGDCVATHAADKPGKYHTISEHMPSHHRAVLAGMN
                     ETVLKERAASIGPPVLAVIDMVLKRSIHPEQAYKSCQGILSLAKKTSKEILIESCAIA
                     LEYQVCTYRNIERLAQGQYANRESLWPTKHAPIPDHPNIRGASHFN"
BASE COUNT         2733 a         2885 c         3246 g         3019 t
ORIGIN      
        1 cagtttgtcc aggcaggggg ggagcagggc gatggcgtcg agcaggaggt ccagcccttt
       61 gtattccctt atgaatccaa agaaaaggac atattggcaa tccgggtcca gccgcagatg
      121 gccgactgct tcggccctgg aaaccgcctg gccataattg tcgtaaatcg ggtgtggaat
      181 cagccggctg ggtttgccgg tcttaaagtc atccaggtca cgcccgactt gttccgacat
      241 atagataaaa cggtcgatgc aagctaaaaa atagcgggta aacagtttgt ctccgggcct
      301 tttttcgtgg gggaccacat tgtcgaggat gctgatggcg agggtgtttt tggtttttgc
      361 tatccggatc acgctggaaa gggctggacc catgagcggg agccagaatt tgcagatgat
      421 gaggtcgggc ttgagacggc gcagtttcag tccggtaagt atccagttga acgggttgaa
      481 ggcgtgcagc caccggtaaa tagtcagggg aggactggga tcctcactca tctgggactt
      541 cccggggaat aaaaaggaag gatattgaac ggtgaaggtg accaggtcga cccggtggcc
      601 catccggatc agttcttccg caagccgctc attaaaggcg gcgagtcctc cccggaaggg
      661 ataggcggtg cccaggatca cgatgtgttt catgccctga tctgcttttc gatctggtag
      721 tggtgtttga ccgccgcatt ccgggtcacc agttccgcga taaatccgga cataaacaat
      781 tgcaggccga cgatgaggaa aaggatgccc aggtaaaaga tcggccggtt tgcaatggaa
      841 ataaaatcaa aaaagaattt cccgatggtg aggtaggtca ggataaaaaa gccgataaaa
      901 aaaatgagga gaccgaaggt gccgaaaagg tgcatgggcc gttttccaaa tttcccgaca
      961 aacatgatgg tggccaggtc caggaaacca ttgacaaacc gttccattcc gaatttggtg
     1021 gtgccgtatt tcctggcctg gtgggcgacc actttttcac cgattttggc aaaaccggcc
     1081 catttggcga tcaccgggat gtagcggtgc atttctccat ccacttcgat gttcttgacc
     1141 acttcctgcc ggtaaatctt gagcccgcaa ttcatatcgt gcaagcggat gccggacatc
     1201 ctgcttgtgg tccagttgta gagtttggac ggaaggtttt tggtgagggc attgtcatac
     1261 ctcttttttt tccatccgga gaccaggtcc agtttttcac ttcggatctt ttccaccagg
     1321 cccgggattt catcggggct gtcttgcaga tcggcatcca tggtgaccac atagtggccg
     1381 cgggtggcat ggaaaccggt gtttagcgcg gcggcctttc cgtaatttct ccggaagcga
     1441 aaagcgcgaa tgttttcatt aaaagcggcc agtttttcaa tcacttccca ggagcggtcg
     1501 gtggagccgt catccacaaa caggatttca tagctgaaat tgttttcccg catgatcctg
     1561 gaaatccagg cttccagctc cggaagcgat tcggcttcat tgtataatgg aataacgaca
     1621 gaaatatcca aggcccctcg gtaaaatttt tacaaagcta atgattccgg ctattaaacg
     1681 gatagtaagg gattcggaaa caccgggggt gttttcaaga caggggatgt tttcgaaact
     1741 ctccctgttt taccaacaaa tcaagagatc tccggcctct ctcttttcat gatcaggccg
     1801 gtgatcaggg cgaggatcag gccgccgatg ccgaaacaaa ccaggttgta ggcaatggtc
     1861 aaaataggac tcttcataaa cgaggtccag gaccgggctg cttctatttg ttcttcgctc
     1921 atgccctggg cttcccaggc gtcttcgatc tgggccatca tcctttcggt aaaacccggg
     1981 tcgatgaact gggtataaac cagcgaaaca agacttgaaa tcaaggtgga cagcaggacc
     2041 acgccgagac ccacgaggat gcattgcccc agattgatat atcctccctg cacttcatcc
     2101 ctttgccgcc ggatagccag taccacaagg gtgaccagga ttgcaaaggt gagaaacccg
     2161 ctcacatagc ccgaggtggg cttgtcgggg ctgctcaatc ccgtaacggt gaagatcagg
     2221 aatatcagag cggaaagaag gcccccggta agtccgaatc tcaggatcac tggtcccggt
     2281 ttagtttctt gcatggaatc ggctgttttg ctgaaaatta gttaaataaa atcaattgtt
     2341 caaataagcg atgcctttat gcaccgtggc caaaaccctg cctttgaagc gttttccggc
     2401 aaaaggggtg ttttttgaaa gcgacttcac ccgttcaggt ccgaattccc attcggcttc
     2461 cggatcgaag accgtaaggt ttgcttccgc acccagttcc atggcaggca gggccagctt
     2521 cagggcccgt cgggaatgaa aagccaccgc gagtacccat tcttcaggct ccagcacttg
     2581 ttcgcttgcg agcgccgcat aagccgtttg taaaccaacg gtaccgggat cggcgtaggc
     2641 gaattcgagg tctttttttt ctgtttccag tggacggtga ttgctgcaga cgatatcgat
     2701 cactttttgt ttgacgccct ggatgagggc cttgcggtcg cgtgcggtgc gcaagggggg
     2761 cgactgttta aaattggaat taaaatcaag gaggctggca tcttccagca gcaggttgag
     2821 gtatgaaacg gatgaaaagg cggaaggcag gattttcctg gccgccttaa attgtttgac
     2881 tgattcttcc gtggaaacca ggtgggcgag atacctgcaa tccgcataag cggccaggta
     2941 cagatcccgc tgcaacatca ggctttcggc aatggatggg attcctttca ggcccagttg
     3001 ctgggaaacg atcccctcgt gcatctggcc caggtgggcc gtggcctggt cttcgggatg
     3061 gttgatgatg aggccgttaa aatggttgac atatcgcatg gccctgagca tcaccccgtt
     3121 gtcctccacg ggaaacaatc catcggaaaa agcgatggcc ccgctggtat gcatatcgat
     3181 catttccgcc aactctttgc cctggcatcc tttgctgata gcccctatgg gatagatatc
     3241 gatatctgcg gcatgggatt tcctggtgat aaattcaatt tccgctttgg aatgcagggg
     3301 gggcttggta ttgggcatca cggccacccc ggtatatccg ccgtcacggg ccgccagggt
     3361 tagactatgc acggtctccc ggtgctcata accgggctcc cccaccgagg cgcccaggtc
     3421 gagccagcct atggagacat gcaggttttt gcctttgatg gttttggccg ttttgtcctt
     3481 accggctttg agtgaaacga tcttaccgtc ccggatccgg atattcaatc tggacccgtg
     3541 ccccggatgt ccggggtgga tgagtttcgc ttgctccagg ataatttcca tgttgacgct
     3601 tatgatcagg ttttccagaa tctgagaaga agggattcga aaagtaagaa taaaagcgtg
     3661 gctgccacaa accacctcca gaaggtcttt ccctggtatt tgtttttgat ccagctttca
     3721 aaatccgtac cgcccgcatt ttccacgatt tcataagcgg acccataagc ttcctccagt
     3781 tcgggaaggg taaaggtcct gaggtcggat tccttgcggt tgtagttgta ggcaactttg
     3841 ccgacgaggg tatcccggag ggtcatttga taaatgccgg catcctgtat tgaacctttc
     3901 aggtccagga tcaccttgct gccggccatg cgttggtagg ggatatagtc ctgtccggcg
     3961 ggccctcctt tcgacaagtg gacgacctct tcagcgctga gcggggtgga ggccgtctcg
     4021 atgatttcat ccctgccgat gaagcggctg atctggtccg cttgtttttt atggacagcc
     4081 attcggaaaa gcatgggaat gaatatttcc ggattccggc tcaggtcgtt ccattcgccg
     4141 gcaagtgggg tggaacatat aaacaaggtg cccccttcgg cgctgagttt ggacaggaag
     4201 ggccgtccat ccgcaaatcc catgatggtc tcctcgccca ggcggccacc ccggcttttg
     4261 agataccggc cctggaccac cgggagccgc atattgctac tcacccggtt atagacttcc
     4321 cggaaggtga attcttccgt attgatcgaa gtcaccgggt aaacgttttt ttcgaaggat
     4381 tgaaattcac cccctcccag ggcctggaca aatccggagt aggaggccag gttggccgcc
     4441 tcgcccggaa acaaaaggac attgctgccc tgccggaggg ctccctggag ttcattggac
     4501 aatccgctgc tgatctcccg aaggccgtcg aggatgatca ggctgttttg agccagggtg
     4561 gagtagttga tattgttcaa ggactggacc ctgaggttcg cggatggaag gctttgcagg
     4621 gcggtcagaa ggtctggatg aatgcggtcg gttgagatta cggtaaggtc gattcttgca
     4681 ttcacgttga aggtcagata aatcttgtca tcaaattgca ctggaaagtc ggtgatctcc
     4741 acctggacct cctgccagcc cggctggttg actacgatgc gcgccgtatc cacgaccgat
     4801 gctccgggtt cgatccgcaa ggtgcccagg ggtttggact ggttgttttg aacgagggaa
     4861 acgcgtgcgt cttcgaccgg ctggtttcca tagttacgta tcctgaattt gagcaggctc
     4921 gcctggccca ggagttggac cggggaatca aaccaggccg aatcgatcgc caggtttgct
     4981 tcctgcacgc tttgcagggg gaggagatgg attttgctga gggtatccgc actgaccgga
     5041 aggtcggtga tcgaccgctg gaaatcggag aggatgtaga tatgttcatt ccccgtttcc
     5101 ttgcgcagga tggactggat acgttcctgg gcatctttca gattatggac atggggggtg
     5161 ggctggattt catcgatcat ggccagggct tcatcctggt ggaccagcct cagttgtttt
     5221 ccctggaatt catgggtgag catgacaaaa cggtcttccc ccgtataagc ttcgacgatg
     5281 tgcctcgcct tttgtttcgc tttggaataa agggggatgt cctccgtgag ggcattcatg
     5341 ctgaaggaat tgtccacaaa aaggcccacc aggttcttgc tgtatttttc cacaccggaa
     5401 gtccttacaa aaggctgggc gaaggccatg accaggaagg caagggccag caggcgcatg
     5461 gccaggacca gcaggttttt cagctttgag cgggcgctgg tctcatcctt tacttccttc
     5521 aggaaacgga cggaggggaa atagactttt ttatacctgc ggaaataaaa taggtggatg
     5581 atgaccggga ttgcaagggt ggcgagcgct gcgagaaacc acggatgaaa aaattgcata
     5641 cgtggcaaaa ataagagaaa gcagttaatc agttcaccag ttaggtaccg aaccagtcag
     5701 tcaattcaac gattcaacga ttcaacaaag gacttagcgg cgctttttac cggtgctttt
     5761 gcttgccagc aatccccagc cgccgctgcc gaataaaaat ccgagggcat accagcctcc
     5821 gttgttgttg ggggcgtaca tgtagacttc cttgtcaaag agggaggcca ggaggtcaaa
     5881 aggggctatc aaaccgtgcc agaggccgcc ccagaaacca taaatatgac ctgaaataca
     5941 gggctccagg ttgttggtgg accgggcgca ggaaaccagg aagaggaata agaccaagac
     6001 gaccaatccg gattttgtat tcattttaag aaaactttat ggaattttaa atttaacagc
     6061 aagtcccggg gaatggttcc tgttgcaaaa agatatcttc aaggaacagt atgcggggat
     6121 taatggttat acttttgaaa aaaaggagaa cggttctatg aattttgtgg attattatca
     6181 gattctcggg gtgtccaaat ccgccacgac ggaagagatc cggaaggctt atcgaaaact
     6241 ggccaggaaa tatcacccgg acatgaacca gggcaataaa gaagcggagc ggaaattcaa
     6301 ggaagtcaat gaagcgaata ccgtcctgag tgaccctgaa aaacgtaaaa aatacgatac
     6361 ttacgggaaa gactgggagc aggcagatgc ttttgagcag gcccgccgca atcagggagg
     6421 ctttggcagg ggcaacgggc gcagccggca aacccgctat accatggatg gggaggatgt
     6481 ggatcccggc atgttttccg attttttcca atccatgttt tttgaccagg gggatttgtt
     6541 cgggtccgga tccttccgga cttcaggggg gacccggcaa cgcagtgctc cccggggtgc
     6601 ggattaccag tccaccttgc aactgaacct ggaggacacc ctccatgaac aaagccaggt
     6661 actcgaagtg aacggcaaaa aaatcaggat aaaaatccct gccggggtgg agcatggcca
     6721 gaccatcaaa gtgaaagggc agggggggca gcatacgccg ggagggccgg taggcgattt
     6781 gttcctgagt tttgaaattc tgcctcatcc caggttcaag accgaaggcc gaaacctgct
     6841 tgccacccac gaactggacc tttataccgc cgtccttggc ggcgagacgg atatccgaac
     6901 cctgacgggc aatgtaaaag taaaggtcaa agccggtacc caacccggtt ccaaaatcag
     6961 actcaagggt ctggggttgc cttattataa gtcggatcaa aaaggggacc tgatccttac
     7021 ttaccaggtg accatacccc aatccctcag ccagaaggag gaagagttgt tcaggaaatt
     7081 gagggaaata caaagcagga cttgactatt ttgacaattg tcattaattt tgtgacagat
     7141 gacaagtaaa tcttatcacg gtgtagaaaa aaaggaaccg gtcataaagg aagccatgct
     7201 gtcggaatat cttggttttc cccatgccgc aagcctgttg tttgcccgct tatttgagtt
     7261 cataggcatc cccaagccca agggcaaaaa tcctcttgaa tccgacctta ttcccctgat
     7321 ccgcaaaggc ctggtcaaaa aacacctgga taagctcatg gacacgacag gccttgatct
     7381 ggcttcgatg gccggcattc tgcatgtgtc ctcccgtacc ctgcaccggt acaccgaaga
     7441 ttccgtactc aatcccgata tcagtgagcg cctgctggag attgcacggc tttatgcgcg
     7501 tggacaggaa gtcttcgggg atctggacac cttcaaaagc tggatccaag agcccgttcc
     7561 cgccctgggt ttcaaaaagc cgctggagtt tctggacacc tcgatcggca tccgcctgct
     7621 cgaagcggaa ttgggccgga tagagcatgg tgttttttcc tgatggaggt cttccgttgt
     7681 tcaaagtgcc gctatatccg ggacctgacc ggggaaggcg cttatttata tggaggccgc
     7741 tggaattcta aaggggtaag actgctctat acctcctcca gcgcggccct ggccttgctg
     7801 gaaaccctgg tgcatacttc ccgcctcctg cccgctatgg attattgcat gctcaggctc
     7861 aggatcgaaa cggattccat ggataccctc cctccctcgg ccatgccgga tgggtggtct
     7921 gataatcccg cgccggatga attaaaacgg attggggacc ggtttgtgaa ggagggaaaa
     7981 tatctgatgt tgcgggtccc ttcggcggtg ttacctatgg agtggaatta tttgataaat
     8041 ccggggcatg atctgttcgg taaggggaag gtggatgagg tggtggagat cagggtggat
     8101 gggagattga ttagttagca agttcgtcag ttagcaagtt cgtcagtcac ccagcactct
     8161 gcgcctcctc tgcgtccttg gcgctctttg cggttaaata tcattaccac gaagccaact
     8221 taccaactta accaacttac caacttgcaa acttagtaaa gcttcttcct cacgcccctc
     8281 gatcccctaa agggggctac cgcgcggttt acaatcaaac taaccaactt gctaacttag
     8341 taaagcttct tcctcacgcc cctcgatccc ctaaaggggg ctaccgcgcg gtttacaatc
     8401 aaactaacca acttgctaac ttactaactt gctaactaaa atcacgcctt cctgcatttc
     8461 tcaatccctt ccaccaatac atccaggtcc gccgggagcg tatagacatt gggggtgatg
     8521 cgcacgccga cgatgttttc ccagttgatc ccggtggtgt ggatcttata tttttcaaag
     8581 aggaaggaat ccacctgggc gggggttttg ccctccacct gcaataaacc gatggcgcag
     8641 ccataggccg ggtcaaggga ggtggccacc cggacaccgg ggatggcatt ggctttggag
     8701 gcccagtagt ttttgaggta atgcagcctt ttttgtttgc gttcgccgcc gatcatctgg
     8761 tgaaactgga tggcctggcc ggtggcctgt tcgatatgga aggggcgggt gccgaggttt
     8821 tcgaatttgc gtatgtcttc gctttccggg tctccggcgg caaagagagg gtagaggttt
     8881 ttgatttttt cttttttgac gatcatcagg cccgtgccaa tgggggcgct gatccatttg
     8941 tgcaagctgg tgccgaaata atcgcagccg atgtccgcga cgtcgtattg gatatgtccg
     9001 aaagagtggg ccgcgtcgag gaccacttcg acattgcgat tatgggcttc gcggacgatc
     9061 tctttggcgg gcatgatctg acccatccag ttgatgatat gggtgaggtg gatcacgcgg
     9121 gtggccggag tgatggcgcc gatatagcgg ttgatgatcg cttccttgtt ttccattggg
     9181 aattcaaaat tgagccagac cagtttgatg ccatcccttt tttcccgctg tttccaggcg
     9241 ttgatcatat tggggtagtc ctgcttgcag aggatcactt catcgccctg cctgagtcgg
     9301 agcccgaaaa tggcggtctc gagggcttcg gaagcgttcc ggtgcaaggc cagttcttcc
     9361 ggggatacgc cggcgagggc tgcgagtttt ttccggaggg gttcccttcc ctggtccagg
     9421 atccgccaca tataatagga gggggcttcg ttgctcaggc gagcgtagcg ttccacggcg
     9481 tcctgcacgg atttgggagc cggggccacg ccgccgttat tgaggttgat gaggttggaa
     9541 tttacggtga aggcttgctg gatcatggcc cagaaatctt cgtcggtggc cagggcatcg
     9601 gcggattggc cggcccggga ccggaaggcg tagtccagtt tttcctgcat ggctttgcct
     9661 gccagggggg cgaggaggga ggcgccgctg aggaggccgg ctttttgtaa aaattgtctt
     9721 cttgttttca ttggtaggat tgtgggtaca aattagggaa attgggcggg tggccaaggg
     9781 gcgggggacc aatcgctcat cctttttttg aggtccattt ttccgtgtaa tattctcacg
     9841 atctctattt cctgcgcctt cattacccga taaaagatga tgtgtttacc tacccgaaat
     9901 cctaatatct gtattgagat ttcaggatag ccccttccca ggtcaggact tttagatatt
     9961 tccttacagg tttcaatcag catttcatag gctgcaacgg cttgattttt cgaccaggtt
    10021 tcatacgtgt aatcccatat ctcagacaag tcttccaccg ctttattggt aagggaaaat
    10081 ttagccattt tttcttttgg cggccttcag ggtttctaaa tgttttttag gattaaagtc
    10141 tttcgctatt ccactgtcca ttcctttccg gatggcattt ttaagttcaa gcaccttgtg
    10201 tttattccgg cgaagctgac ccccattccg gcatattgac ccctctagcc aggagtactt
    10261 tcagccatct attccggcat attgaccccc ctgatgtcaa acgaggagtt ggagtgtaat
    10321 gtcaaagaac gaggataaag ttattttctt tttcttaaag attctccagt aagttcaatg
    10381 cgatgtgaat tggatacaat gcggtccagg attgcatccg cgatggtcgg ttctgctatg
    10441 atttcgtacc agatgctgat aggtaattgg gaagcaatga tcatcgatct acgcccgtgc
    10501 cgatcctcca ccatttccaa aagatccaat cttgccaatt catcgggtcg ttgtaagcca
    10561 aagtcatcca gaatcagtag atctgcacga gccagtagtt gcatttgcct tctgtgcgtt
    10621 ccatcgagcc gagctaagcg aagagcctga tatagttttt gcgtgttgtg gtatgccacc
    10681 ttcaacccca tttgacaaca ttgatgtcca atagctgatg ccagatgact tttccctact
    10741 ccggttggtc ctgtaatcat cagattttcc gctttgttga cccaatcaca ggtactgatt
    10801 aaggcaagtt gttctccaga tagatttcgt ttcgcatcgg ctttaatctc ctggagcgat
    10861 gccgtgtaac ggaatcgggc cgaacgtgtc aaacggtgta ttttccggtt attgcgttca
    10921 tcccattcct gttgcaacag tatggccagt aattgatctg cattgatgct tcccagtttc
    10981 ttgccttgaa cgagtccttg catcgcatca tacataccat gcaatctcat gtcgcgcatt
    11041 aattcaagtg ttttttgatg attcattgta catgtatttt taaagtgatt aattaaagtg
    11101 agaggcaccc ctgatattgg gatggtctgg gatcggagcg tgtttggttg gccacagaga
    11161 ctcccggttg gcgtattgac cctgtgccag ccgttcgata ttccggtaag tacagacctg
    11221 atactccaat gcgatggcac agctttcaat caggatttct ttggatgttt ttttagccag
    11281 tgacaagatg ccctgacaag atttgtatgc ttgttccgga tgaatacttc gtttcaatac
    11341 catatcgatg accgccagca ccggtgggcc aatagaagca gccctttctt ttaataccgt
    11401 ttcattcata ccggccaaca ctgcccgatg atgggagggc atatgctcac tgatcgtatg
    11461 gtacttaccg ggtttatctg ctgcatgggt ggccacgcag tcaccctggt gatagattct
    11521 taccagggaa ggagtaatga ttacgtgtac tttcagacca atgtaacgat aaggcgcact
    11581 gaagtatctt ttggattttg acagaaatac atggccattc tgctggacgg ttagaatcag
    11641 gtgttgcttg atttgatagc gttcattggg caatgccttg agcgcgggtc tttcatccct
    11701 ctccaacagt acttcccgtg aacattggta ctgttgcatc attcgttgat tgagcaaagt
    11761 cgattgttcc agcagggccg tattcaattc ctgcacgctg taaaaggtct gcttccgtaa
    11821 gggagcgaat aacgccaagt aggaacgggc cgcggcccgt tccacttttg ctttgtcttt
    11881 ggg
//
LOCUS       DAOQXT010000126        11739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_5937_length_11739_cov_6.320181, whole genome shotgun sequence.
ACCESSION   DAOQXT010000126 DAOQXT010000000
VERSION     DAOQXT010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5937_length_11739_cov_6.320181"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..709)
                     /locus_tag="PKM27_16830"
     CDS             complement(335..709)
                     /locus_tag="PKM27_16830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR08987.1"
                     /translation="MRTDILPGKYLVTLTDSKNCTGVASKEVFLRTATNCQTTSIQPD
                     AAPQPEIWPNPFLSKIRVSNPSGRGVSLRLIDMMGIALYGVKLTGIQTEIDLNWLPTG
                     IYVLILSDSNNQVIKLEKMVKR"
     gene            complement(673..3267)
                     /locus_tag="PKM27_16835"
     CDS             complement(673..3267)
                     /locus_tag="PKM27_16835"
                     /inference="COORDINATES: protein motif:HMM:NF021945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNR08988.1"
                     /translation="MRDLFYIAVIVTWTPALIHGQSILGSVRPNTNTALPASYGFMEY
                     VFPEPADQGVCRGQKNASTLLRGIFFAQTHRLSVDHPYFFLTGYRPALLQVAVTGQGP
                     SPDIQVEGLRNGNSLGGLCLKGPALLPGEIDLKVPGFEKYFTVTLPGEWIRPGTELIV
                     KYGDQSKKLSSDMLKIGPYTELNLVHYDLELLDYPGEPNKSIDHLLAEIASACPASVI
                     RMGKFPVKLEFPEVIANNDREQLVRLKSRRLMAGNNIFSDGYINSVATQFLYNLHRST
                     QDYLSTFYFGNTLNLKPGGWGGAKSFVGFDYDDVFIHEMGHAFSLPHWGLAYGIPNPA
                     EWDYIYPYGGDTGDGGGRGESWTFLQHLYEFVNPVCQYDARGKAGWEASDAMQRNNHC
                     LQARSYGPGPWDGFGDFSALAMSRYLAGSDELYQGTVSYRGNPIPFQFNRQPGFPILT
                     LENGQRTYRRDPLQPQAMVYEEQMLVLGNEKPNTPVYLIYGSAHETQTQANIIYKPVK
                     FTGTLPPVVDPTDPATFNKIKSASWRPFFEQPRDITLKMTYADGSVLHAVNPYHSYTR
                     TADYQWGYHIWRNDICHFSLVVPGNKELIKVELFNRPLVARDAGDPIRGNINNASQNI
                     TSGNFMNGATLQAVYPGERPLALNSIGDLVWHDLNRNGKEDFGEPGIPGVTLLLWEDS
                     DDDKILDYLGYKGSTVTDSGGRYRFEGLLPGHYLVFVWSLENWNQGQALHGMVSTKAV
                     SDPNTDIDSDNNGRPGIPFGLTGQDIVSGIIDLTADGEPLDDHDRPSGWFDYNPSGNM
                     SVDFGFYFPAGCPVINAYLLGTDSLCVQENGNLTVDPASGTGPFSYTWQDGSNTACVR
                     IYFQASTW"
     assembly_gap    3635..3734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4024..6258)
                     /locus_tag="PKM27_16840"
     CDS             complement(4024..6258)
                     /locus_tag="PKM27_16840"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNR08989.1"
                     /translation="MNSSNKINPKTGRGPKGLDRRSFLKLSGLAGPGLLIGIHLPDRA
                     MEVSGSAPANSFEPNVFLKIGIDGTIIIKAKNPEIGQGVKTSLPQIIAEELEVDWKQI
                     TVEQGDYDQRFQGQFAGGSTGIKDNFLPLRSAGAAAREMLIEAASRKWQVPVNQCRAA
                     SGFVHNQNNGEKYSYAALAEDAAKLPYRENPKLKDPKDFKLIGKALPGVDNPGITTGK
                     PLFGLDARPVGMLVAVIARCPVRGGKLKSFDASKSLAFPGMKEVLDLDLPQADPRVSV
                     HGVAIVADHTWAAIKGREALQVEWDFQGGEQENDERIQSAFETNIKNPGSIILRLDGK
                     PEEAFDKASRKWEAVYQVPFLSHAPMEPMNFIADVRADSCEVWGPTQVPGSVRRHALA
                     ITGLPADKIKVRMTRSGGGFGRRLSADYATEAILLSKKLGKPVQVIWTREDDMKNDLY
                     RPAGMYKVRGSLDEQGKLSSWQLNASTTSRYLYAGSTASPHTTEVFPDGFPAGFVPNF
                     KMEYTPVRTNIGTGAWRAPGHNATAFVDQSFVDEMAHLAGRDPVDFRLEILGDGDKIM
                     PYRDHGGPEYSTARLKGAIQLAVEKSDWYGPKREGIFKGFACHFMFGAYVAEVVIVSL
                     KGGRVKIEKVICALDCGLVVNPSGARNQVEGGIIDGLQTALSSAIHIEAGSSREKNFD
                     AYRMLRMNEIPDVDVYFVDSREHPEGLGEMSLPAVSAALCNAVFAATGKRIRKLPFDL
                     ASLV"
     gene            complement(6275..6727)
                     /locus_tag="PKM27_16845"
     CDS             complement(6275..6727)
                     /locus_tag="PKM27_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR08990.1"
                     /translation="MATYTLLINGNSHTFQSEPDRPLLWVLRDTLELKGTKYGCGIGF
                     CGACTVHIGETAVRSCSIPVSAVQGAVTTIEGLSRDGSHPLQLMWMEMDVAQCGYCQA
                     GQIMSAAALLHRNKNPNREEIHAAMQGNLCRCGTYLRIRQAIERVLLK"
     gene            6973..8025
                     /locus_tag="PKM27_16850"
     CDS             6973..8025
                     /locus_tag="PKM27_16850"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNR08991.1"
                     /translation="MPLNTAPDGGNKKASVSEQIGIVKINIHYSRPGVKGREGRIWGT
                     SVAHYGLQDLGHGTCYAAPWRAGANENTTFSFSHPVKIEGKDLPAGSYGFFIINEEKE
                     STLIFSKNSTSWGSFYYKPEDDALRVTVKNQPLEKSEEWLKYEFTDQTDSSATVSLSW
                     EKRRIPFRISARIHELQIAEFKKDLLTTRPPDDFVQAANYCLENNIELEQALAWIDRG
                     IYFRIMGQKTFRTLSTKAALLKKLNRDQEAELIMQEALPLGSMTDVHFYGRRLLAAKK
                     AEEALKVFQNNYDKYPNQFTTTMGMARGLSAIGNYNKALEFMKSALPMVPDAANKANV
                     ENAIIKLQKGMDFNIE"
     gene            8073..8510
                     /locus_tag="PKM27_16855"
     CDS             8073..8510
                     /locus_tag="PKM27_16855"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR08992.1"
                     /translation="MKLKNIWYLLCFFFFPCPISFSQINKDEAAILKVFQEEERTWNE
                     HDLEGYVALYAPLDSTRMILSKGVAAGKENILAFYKKYWPPEKMGNLKLTNESLERIS
                     KKYYYVTGYFQVSYPDGKMTHGRYSGLMKKWKGKWYIYTDHSG"
     gene            8666..9190
                     /locus_tag="PKM27_16860"
     CDS             8666..9190
                     /locus_tag="PKM27_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08993.1"
                     /translation="MKWSEEKGQDVIELLLNEDEGLAWIKGIEFKTGTIELRLKGIDV
                     YQHSFLGIAFHGVNDSTFDALYFRPFQFLAEDTVRKKRAIQYISLPQYTWSRLRETQN
                     GKFENQVVPAPDPNDWFHARFIISETEARVYVNDTPEPYLIVPLLGAKSGKIGLYTAY
                     RSGGSFANLRIIKG"
     gene            complement(9303..9764)
                     /locus_tag="PKM27_16865"
     CDS             complement(9303..9764)
                     /locus_tag="PKM27_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08994.1"
                     /translation="MKKIVFIDMDNVLVDFQSGIDRLTDEQKQEYTERYDEVPGIFSL
                     MDPVPGSIESVKTLSDHFDLYILSTSPWKNPSAWSDKLLWVQKYFGAEENSLFYKRLI
                     LSHHKNLIAGDFLIDDREKNGANEFKGELILFGLGKFPDWKKVTEYLLTVS"
     gene            10096..>11739
                     /gene="rmuC"
                     /locus_tag="PKM27_16870"
     CDS             10096..>11739
                     /gene="rmuC"
                     /locus_tag="PKM27_16870"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNR08995.1"
                     /translation="MTESTMFILIIVLQVLAIILIVVFRHKEDKALPAIQNKLSELQA
                     GLDKLEHNFKEDFRLNREENSGIAGNNRAELNATLLGIKAELTTLLHASGQQNQNALK
                     EINNTLADKVEALTLQIVNLNRNNREEFASGMNSFTKEQKGKFDELKTEQKELTARTT
                     ELLSKVSDKVEEKLAKLNDQNKSDNSLMRGELTASFRSFNEAFEKSIAAFSQLQKEKF
                     GEVEAKQNELIKNTESKLDLIRSTVEEKLEKTLSDRLGQSFDSVGKQLIEVQKGLGEM
                     QTMAADVGGLKKVLSNVKLRGGIGEVQLAMLLEQILAPSQYEANVATVEGSTERVEFA
                     VKLPGRNNDENSFIYLPIDAKFPKESYEQLITAYENAIPDDIEAATKNLENTIRKMAK
                     DIRDKYIGPDTTDFAIMFLPFESIYAEVIRRSALVDQLRNEYKITVAGPTTLMALLNS
                     LQMGFRAMALQKRSGEVWKVLARVKSEFEKFGGLLEKAQKNIQTGLGQLETASGIRTR
                     AIQRQLNTIESMPVPAISMGSSGEVQEDLTVDDEIWEENN"
BASE COUNT         3016 a         2796 c         2923 g         2904 t
ORIGIN      
        1 cgaagggtta ggtcatgctt tatattttct tggtggtctt cgtgtcttcg tgggcggcaa
       61 ctttgtgtcg cagtgtgaat gagccgctac gcggctttcc cgctatggtt tcccacgaag
      121 acacgaagga cacaaagggg aaggtgatcc cttacatttt cttggtggtc ttcgcttcgc
      181 tgtgtctttg tgggcgccaa cctttgtgcc gtcgtgggaa tgagccgcta cgcggcttac
      241 ccactgagat ttcccacgac gacacagcga agcgaaggca attttgctaa gcagactttc
      301 gccgcacggg aagagtcctg gaatccggtt ccctttaccg ttttaccatt ttttcaagtt
      361 taatgacttg gttgttgctg tcggataaga tcaaaacata tatccctgtg ggcagccagt
      421 tcaaatcgat ctcagtctga atgcccgtta gttttactcc atatagggca atgcccatca
      481 tatcgattaa acgcagggat accccccggc ctgagggatt ggaaacccgg atcttgctca
      541 agaaaggatt gggccagatc tcaggctgtg gggccgcatc gggctggatc gaagtggttt
      601 gacaattggt cgccgtacgc aggaacacct ccttgctggc tacaccggta caatttttgg
      661 aatccgtgag ggtcaccagg tacttgcctg gaagtatatc cgtacgcacg ccgtgtttga
      721 cccgtcttgc caggtataac tgaacggccc ggtgccactg gccgggtcca ccgtcaaatt
      781 gccgttttcc tgcacacata gcgagtcggt accgaggagg taagcattga tgaccggaca
      841 gcctgccggg aaataaaacc cgaaatccac ggacatattt ccggaaggat tgtaatcaaa
      901 ccagccggat ggacggtcat ggtcatccag gggctcaccg tcggcagtca ggtcaatgat
      961 gccggagacg atatcttgtc cggtcaatcc aaacgggatc ccggggcgtc cgttgttgtc
     1021 tgaatcgata tccgtgttgg ggtccgatac ggccttggtg ctgaccatcc catgtaaggc
     1081 ttgtccctga ttccagtttt caagactcca cacaaatacc aggtaatggc caggcaaaag
     1141 gccttcgaag cgatatcgcc cgccggaatc ggtgaccgtt gatcctttat aacctaaata
     1201 atccagaatt ttatcatcgt ccgaatcttc ccacagtagc aaggtcaccc ccggaatacc
     1261 gggttcacca aagtcttctt taccattacg gttcaggtcg tgccagacca ggtctcctat
     1321 cgaatttaga gcaagaggcc tttcgccggg atacacggcc tgtaaggttg cgccgttcat
     1381 aaaattgccg gacgtgatgt tctgggaagc attgttgata ttgcctcgaa ttggatctcc
     1441 ggcgtcccgg gccaccagtg gccgattaaa caattccact ttaatcaact ctttatttcc
     1501 cgggaccacc aggctgaaat gacaaatgtc gtttcgccaa atatggtaac cccactgata
     1561 atcggcggtt cgggtataag aatggtaagg attgacggca tgcagtacgc tcccgtccgc
     1621 ataagtcatt tttaaggtaa tatccctggg ttgttcaaaa aacggacgcc aggatgccga
     1681 cttgatttta ttgaaagtgg cggggtccgt ggggtcgacg accgggggaa gggtgcctgt
     1741 gaatttgacg ggcttataaa taatattcgc ctgggtttgg gtttcgtggg cgctgccgta
     1801 aatcaggtat accggagtat ttggtttttc attgcccaat accagcattt gctcctcata
     1861 aaccattgct tgcggctgca agggatctct gcggtaggtc cgctgaccgt tttcgagggt
     1921 gaggatggga aatcccggtt gacggttaaa ctgaaagggg atcgggtttc cccggtagga
     1981 gacggtcccc tggtagagtt catcgctgcc tgccaggtac cgggacatgg ccagggccga
     2041 aaaatcgcca aaaccatccc aagggccggg cccgtaactt cgggcttgaa gacaatggtt
     2101 attgcgttgc atggcatccg atgcttccca accggcttta ccccgagcat catactggca
     2161 taccgggttc acgaattcgt acaaatgttg caaaaaggtc caggattccc cgcggccgcc
     2221 gccgtcgccg gtgtctccgc catagggata aatgtagtcc cattcggcag gattgggaat
     2281 tccgtaagca agaccccagt gcggcagact gaatgcatga cccatttcgt gaataaatac
     2341 gtcatcgtag tcaaaaccca caaacgattt ggctccgccc catccgccgg gtttcaggtt
     2401 gagggtattg ccaaaataaa aagtggaaag gtagtcctga gtgctgcggt gaaggttata
     2461 taaaaattgg gttgcgacgg agttgatgta cccgtcgctg aatatattat ttccggccat
     2521 cagccttctg gatttcagcc ggaccagttg ctcgcggtcg ttattggcga tgacttcagg
     2581 aaattccagt ttaaccggaa attttcccat gcgtatgaca ctggccgggc aagccgaggc
     2641 tatctccgcc agaagatgat ctatgctttt attgggctcg ccgggataat ccagcaactc
     2701 caggtcataa tggaccaggt ttaattcggt atagggaccg attttaagca tgtcggagga
     2761 caattttttt gattggtcgc catattttac gatcaattcg gtccccggcc ggatccattc
     2821 cccaggaagg gtgaccgtaa aatatttttc aaagccgggc accttcagat cgatctcacc
     2881 gggaagcaag gcgggccctt tcaggcataa ccctcccagt gaatttccgt tgcgtaatcc
     2941 ttcaacctgg atatccggtg acggaccctg cccggtgacc gccacttgta agagtgcggg
     3001 gcggtacccg gtaaggaaaa aataaggatg gtccacggac agccgatggg tctgagcgaa
     3061 aaagatgccg cgtaaaaggg tagaggcatt cttttgacct ctgcagactc cctggtcagc
     3121 cggttccgga aaaacatact ccataaatcc gtacgaagcc ggaagcgccg tattggtatt
     3181 gggacgaact gagcctaata tggattgacc atggatgagc gccggagtcc aggtcacaat
     3241 aacggcaata taaaataggt ctctcatagt tatcattttt cactgattta tttccgcccg
     3301 gagtaaactt cgctgaataa tacgatgatg aatataccga taataattaa gtactgggtc
     3361 attaatgggt tatatttcgg cctctgataa tcctgtgcat tccgaaataa atttattgga
     3421 tccgtctctg tatttattcc tgtaagggca aggaagattg ttttgtttga gtatgctgcc
     3481 aaaatccttg gttcccccgt gggcttgccc caatagtaat atttgaaaat tagtaatcgg
     3541 ggggacaggg gtgttggaga tattaataag ttgaccccag cccctgaccc ctcaagggga
     3601 acctgacccc tggcttagga acgaatttta attgnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnngggggc ttgccccaac tgtaggattt gaggattagt cgtcgggggc
     3781 ttgccccaat agtaggattt gaggattagt cgtcgggggc ttgccccatt agtaatattt
     3841 gaaaattggt agtcaggggg gaaaggggtg ttggagatac taataggttt tctcaggccc
     3901 ctgacccctg aaaggggaac ctgacccctg gcattcagaa ataaatttaa ttgacttatc
     3961 tcagcgctca tttcggcaag ggcaaggaag attgttttgt ttgagtatgc tgccatgatt
     4021 gactcaaacc aggctggcca gatcgaacgg aagcttcctg atccttttac ctgtcgcggc
     4081 aaagacggcg ttgcacaaag cggcgctcac ggcgggcaat gacatttcac ccaggccttc
     4141 cgggtgttcc ctgctgtcca caaagtacac atcgacatcc gggatctcat tcatgcgaag
     4201 catcctgtag gcatcgaagt ttttttccct tgagctccct gcctcaatat ggatggctga
     4261 actcagggcg gtctgcaaac cgtcgatgat gcctccttcg acctggttcc tggctccgct
     4321 tggattgacc accagcccgc aatcaagggc gcagattact ttttcgattt tcacccggcc
     4381 gcctttgagg gaaacgatga ccacctccgc cacataggcg ccaaacataa agtggcatgc
     4441 aaatcccttg aagatgcctt ctcttttcgg gccataccag tccgattttt caacggccag
     4501 ttggatcgct cctttcaacc ttgccgtgct gtactcgggc cccccatggt cccgataggg
     4561 catgatttta tccccgtcgc ctaaaatttc aagcctgaaa tccacggggt cccgcccggc
     4621 caggtgtgcc atctcatcca caaaactctg atcgacaaag gcggtcgcat tatgccccgg
     4681 cgccctccag gcgccggtcc cgatattggt cctaacggga gtgtattcca ttttaaaatt
     4741 gggaacaaat cccgcgggga aaccatccgg gaatacttcg gtcgtatgtg gggatgcggt
     4801 gctcccggca tagagatacc tcgaggtggt gctcgcattc aattgccagg aggagagttt
     4861 accttgttca tccagggatc ccctgacttt atacatcccc gctggccggt acaaatcatt
     4921 tttcatatca tcctccctcg tccagatcac ctgcaccggc ttgcccagtt ttttggaaag
     4981 caggatcgct tcggttgcat aatccgcgct caacctcctt ccaaatcctc cgcccgaacg
     5041 ggtcatcctg accttgattt tatccgccgg caacccggta atggcgagcg catgccttct
     5101 gacggatccg ggtacctggg ttggtcccca gacttcgcag gaatccgccc tcacgtcggc
     5161 gatgaaattc atgggttcca tgggagcgtg cgaaaggaag gggacctggt ataccgcttc
     5221 ccatttacgc gaggctttat caaatgcttc ctccggcttg ccgtccaggc ggagtataat
     5281 ggatccgggg ttttttatat tggtttcaaa ggcggattga atgcgttcat cgttttcctg
     5341 ttcaccgccc tggaaatccc attccacctg cagggcttcc cttcctttga tagccgccca
     5401 ggtatggtct gccacaatgg cgactccatg aacggatacc cggggatccg cctggggcag
     5461 atccaggtcc aggacttctt tcatcccggg gaaggccagg gattttgatg catcaaagga
     5521 tttcagtttg ccaccgcgga ccgggcaccg ggctatgacc gccaccagca tgcccacagg
     5581 acgggcatcc aggccaaaga gtggctttcc ggtagtgatt ccgggattgt ccactccggg
     5641 cagggctttg ccaatcagct taaagtcctt gggatccttg agcttaggat tttcgcgata
     5701 ggggagtttg gcggcatctt ccgccagcgc tgcatatgaa tatttttcgc cattgttttg
     5761 attgtgcaca aaaccagagg cggcacggca ttggttgacc ggtacctgcc atttacggga
     5821 ggctgcttcg atgagcatct cgcgggcagc ggccccggca ctgcgaaggg gtaaaaaatt
     5881 atcctttatg ccggtgctgc ctcctgcgaa ttgtccctgg aacctttggt cataatcccc
     5941 ctgttccacg gtgatttgtt tccaatccac ctcaagttct tcagcgatga tttgagggag
     6001 tgaggttttt actccctggc cgatctctgg attttttgct ttgatgatga tggtcccgtc
     6061 gatgccgatt ttcaggaata cgtttggctc aaaggaattt gccggggcgg atcccgaaac
     6121 ttccatggcc ctgtccggaa ggtgaatgcc gatcagcaaa cccggccctg ccaatccgct
     6181 gagttttaaa aacgatcttc ggtcgagccc cttaggtccc cggccggtct ttggattgat
     6241 tttatttgat gagttcatag atcattgcct tcggttattt taaaaggacc ctttcaatag
     6301 cttgcctgat ccttaaatag gtgccgcagc ggcaaagatt gccttgcatg gccgcatgga
     6361 tctcttcccg gttgggattt ttattcctgt gcagcaaggc ggccgcggac atgatctgac
     6421 cggcctggca ataaccgcat tgggctacgt ccatttccat ccacatgagt tgaagcggat
     6481 gggaaccgtc ccgggaaagg ccttctatcg tcgtgaccgc gccctggacg gcggagaccg
     6541 ggatactgca ggagcgcacc gcggtctccc cgatatgcac cgtgcaggcg ccgcaaaagc
     6601 cgatcccgca cccgtatttg gtccccttca gttccagggt gtcgcggagc acccacaaca
     6661 agggcctgtc cggttccgac tggaaagtgt gggaattgcc attaatcaat aaggtataag
     6721 ttgccatagg cctggattga tcatcaggga aataaaagaa tgaatgattt tcgggagtaa
     6781 gtccaggggc taaattatgg tatttcaaaa tatgggtaat cccccccgga gggggaggag
     6841 gatccattac aaagtgttga attttgaccg gttcaattca atttaaactt ctgaaataaa
     6901 cctctctcaa tgaaaaaaat aatcctgttt atcctgggga tttattccgc ttccatggcc
     6961 cggacctaga tcatgcccct caatactgcc cccgatgggg gaaataaaaa ggcctccgta
     7021 agtgagcaga ttggaattgt aaaaatcaac atccattata gccggcccgg tgtaaaaggc
     7081 cgggaagggc ggatttgggg tacttccgtt gcccattatg gcttacaaga tctgggtcac
     7141 ggaacttgtt atgccgctcc ctggagggca ggggccaatg aaaacaccac tttttcgttc
     7201 agtcacccgg tgaagattga gggcaaagac ttgcctgcgg gcagctatgg attttttatt
     7261 atcaatgagg aaaaggaaag caccctgatt ttctcaaaaa attcaacgtc ctgggggagt
     7321 ttctattaca aaccggaaga cgacgccttg agagtgaccg taaaaaacca gcccctggaa
     7381 aaaagtgaag aatggcttaa atatgagttt acggaccaga cagacagttc ggccaccgta
     7441 tccctttcct gggaaaaacg caggattcct ttccggattt ccgcccggat ccatgagctt
     7501 caaattgccg agtttaaaaa agacctcctg actacgagac ctccggatga ttttgtacag
     7561 gccgccaact attgtctgga aaacaacatt gaactggagc aggccctggc ctggatcgac
     7621 cgcggcattt atttcaggat aatgggtcaa aagaccttcc gcacgctgag caccaaagcg
     7681 gctttgctga agaaactgaa ccgggatcag gaggccgagc tgatcatgca ggaagccctt
     7741 cctctcggca gcatgaccga tgtgcatttc tatggccgta gattgctcgc tgcaaaaaaa
     7801 gcggaagagg cgctgaaagt ctttcaaaac aattacgaca aataccccaa ccagtttacc
     7861 acaactatgg gaatggccag aggtttatca gctataggga attataataa ggccctggag
     7921 tttatgaaaa gtgctctgcc catggtaccg gacgcagcca ataaagccaa tgtggaaaac
     7981 gcaataataa aacttcaaaa ggggatggat ttcaatattg aataaatggc cttgtgataa
     8041 aggatttttt ctgaaatgcc caaaaattta aaatgaaact taaaaacatc tggtacttat
     8101 tgtgcttttt cttttttccc tgccccatat cttttagtca aattaataag gatgaagcgg
     8161 ctattctgaa agtttttcag gaagaagaac ggacctggaa tgaacacgac cttgaaggat
     8221 atgtcgcatt gtatgctccc ctggattcca cccggatgat cctgtcaaaa ggggtagcgg
     8281 ctggtaaaga aaatatcctg gccttttaca aaaaatattg gccccctgaa aaaatgggaa
     8341 acttgaagct taccaatgaa agcctggaac ggatctcaaa gaaatattat tatgtgaccg
     8401 gctattttca ggtgagttac ccggatggca aaatgaccca tgggcgttat tcgggtctga
     8461 tgaaaaaatg gaaaggcaaa tggtatatat acaccgatca ttcaggctga tgaggacatc
     8521 aataaataac ataagatact tcatactcgt gctgggcatt tttattggtt taccggtata
     8581 caaccaaaca taccaacaga aactaattga aatagacctt aaatcgcttt atgaaaaaaa
     8641 tgaattgacc ctggttaacc gtcaagtgaa atggtcggaa gaaaagggtc aggatgtcat
     8701 tgaattactg ctcaacgaag atgaaggttt agcctggatt aagggaatag aatttaagac
     8761 ggggaccatt gagctgcgtc taaaagggat tgatgtctac caacacagct tcctgggaat
     8821 agcctttcac ggcgtcaacg attcaacctt tgacgcgctg tatttcaggc ccttccagtt
     8881 tcttgcggaa gatacggtcc ggaagaagag agctattcaa tacatcagct tgcctcaata
     8941 tacctggtcc aggctccggg aaactcaaaa cgggaaattt gaaaatcagg ttgtcccggc
     9001 tcctgacccc aatgactggt ttcatgccag gtttatcatt tcggaaacgg aggccagggt
     9061 ttatgttaat gatacacccg aaccttacct tattgtccca ctattgggtg ccaaatcggg
     9121 taaaatcggc ctctatactg cataccgctc cggcggttca tttgcgaatt tgaggattat
     9181 caaaggatag ccggggcagg aagttaataa cctgaagatt ggaacaggag aagggaatcg
     9241 tcattttgcg ctttcaggac tcctggaaat ccgtaacggc ccggtagggg catcccggca
     9301 attcaactca ctgtcaataa atattccgtg acttttttcc aatccgggaa tttgcctaat
     9361 ccgaatagga ttaattcacc tttaaattca tttgctccat ttttctctcg gtcatcgatc
     9421 agaaaatcgc ccgcaatcag gtttttatga tgagatagta ttaacctttt gtaaaacaaa
     9481 ctgttctcct cagctccaaa atatttttgt acccataata atttatccga ccaggcagat
     9541 ggatttttcc agggtgaagt tgataatata tataagtcaa aatgatccga cagtgtttta
     9601 acagactcaa tggacccagg caccggatcc attaagctga atattccggg aacttcatca
     9661 taccgctcag tatattcttg tttctgttca tcagtcagcc ggtcaatacc ggattggaaa
     9721 tccaccagga cattgtccat atcaataaat actatttttt tcatgtaaaa tctgtttctg
     9781 attatgttat taatctttat tcagtgggcc acaaattact cttcccgatc cggatcttgg
     9841 cgagtcatgc ccttgggttc ttataagtaa ttgaattcca actatgtgta tatgatttca
     9901 gtcagtgatg tcctgggtca gactttttac gaatatccgt tctactgttt ccggatctgg
     9961 tattattaga gaagaaggca tcttcggtta ccagccctga cgaaaaggcg gaagcggtat
    10021 ggaacaggtc ccgatttata aaagtactcg tagaaaaacg aagtaacttt gatataacgg
    10081 cttataacaa taccaatgac cgagagcaca atgtttatcc tgatcatcgt tttgcaggta
    10141 cttgctatca ttttaattgt agttttcaga cacaaagagg ataaggctct gcctgccatt
    10201 cagaacaaat tatctgaatt gcaggccggc ctggataaac tggaacataa ttttaaggaa
    10261 gacttccgtc tcaaccgcga ggagaattcg ggcattgcgg gcaataacag ggccgaattg
    10321 aatgccaccc tgctcggcat caaggccgaa ctgaccacct tgctgcatgc ttccggtcag
    10381 caaaatcaaa atgccctgaa agagatcaat aacaccttag ccgacaaggt ggaggccctg
    10441 accttgcaaa tcgtaaacct caaccggaat aaccgggaag aatttgcctc cggcatgaac
    10501 tccttcacaa aagaacaaaa agggaaattt gatgaattga aaaccgaaca aaaggagttg
    10561 acggcacgga ccaccgaact tttgagtaag gtttcggaca aggtggaaga aaagctcgcc
    10621 aaactcaatg atcagaacaa gtcggacaac agcctcatgc gcggggaact caccgcttct
    10681 ttcaggagct tcaacgaagc ctttgagaaa agcatcgccg cgttcagcca attgcaaaag
    10741 gaaaaattcg gcgaggtgga ggcgaagcag aatgaactga taaaaaatac ggaatccaaa
    10801 ctggacctta tccggtccac cgtggaggaa aaactggaaa agaccctgag cgaccgcctg
    10861 gggcagagtt ttgattccgt cggcaaacaa ttgatagaag tgcaaaaagg cctgggcgaa
    10921 atgcagacca tggcggcgga cgtaggaggg ctgaagaaag tattgagcaa tgtaaaatta
    10981 agggggggca ttggggaggt gcaactggcc atgttattgg aacaaatact cgcgcccagc
    11041 cagtatgaag ccaatgtggc gacggtggaa ggcagcacgg aaagggtgga atttgccgtg
    11101 aaactaccgg gacggaacaa cgatgaaaac agcttcatct acctgccgat cgacgccaaa
    11161 ttccccaaag aatcctatga gcaattaata acggcctatg aaaatgcgat tcctgatgat
    11221 attgaagccg cgaccaaaaa cctggaaaac accatccgga aaatggcaaa agacatcagg
    11281 gataaatata tagggcccga taccaccgat tttgccatta tgtttctgcc gttcgaaagc
    11341 atttatgccg aagtgatccg gcgaagcgcc ctggtcgacc agctaagaaa tgaatacaaa
    11401 ataacagtcg caggtcctac caccttgatg gctttactga acagcctgca gatgggtttt
    11461 cgcgccatgg ccttgcagaa aagaagtggc gaagtctgga aagtattggc ccgggtcaaa
    11521 tcagaatttg aaaagttcgg cggcttactg gagaaagcgc agaaaaatat acaaaccggt
    11581 cttggccaat tggaaaccgc ctcggggatc aggaccaggg cgattcaacg gcagttaaat
    11641 actattgaat cgatgcctgt tccggcgatt tccatgggct cctccggaga ggtccaggag
    11701 gacttgactg tagatgacga aatttgggag gaaaataac
//
LOCUS       DAOQXT010000127        11382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6142_length_11382_cov_6.581354, whole genome shotgun sequence.
ACCESSION   DAOQXT010000127 DAOQXT010000000
VERSION     DAOQXT010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6142_length_11382_cov_6.581354"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..558
                     /locus_tag="PKM27_16875"
     CDS             55..558
                     /locus_tag="PKM27_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08996.1"
                     /translation="MTLKQFFDFISEHPSLILFYSVAVPLSAILTGLFSRGEGHLAPW
                     KYLYAFLVYFVCIPGIFAVTLDVYLFLFERQSIWESNLYTQFLPVLVMLITLMIIKRF
                     VRLESIPGFGRLTGLILMISAVLVAMWFLDKTHIFVFSYMPFYQVVLLLVVLFVVLRL
                     AWRKWAG"
     gene            648..1661
                     /locus_tag="PKM27_16880"
     CDS             648..1661
                     /locus_tag="PKM27_16880"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNR08997.1"
                     /translation="MKALKYIGLLLLTLLALFLVLGLVSTKKVHLERTTLIKAPVSTV
                     QDVVRRFSTQKAWNPWQEYDPEMKVTLEGEDGSVGSKYSWSGNKDVGKGYQMITNIAP
                     DYVGSELVFEEPWSSKATIGMKLTEEDGATRATWSFDSETPFPWNAMNLFMNMDKLLG
                     PDFEKGLNKLKTYIETEVDKKYRGYTINESEHPARFFVGMRKTVAIPQITSFFAEQFG
                     KIYAAIQKAGIEMDGMPAGLYFTYDETAGTTDMVAGIPVKGKASVPGFSTFEIPAGTE
                     LSLDYFGAYDKMGEAHYAMEDYIREKALTGRVPVIEEYLSDPELEPDTAKWLTRVIYP
                     VSK"
     gene            complement(1674..3134)
                     /locus_tag="PKM27_16885"
     CDS             complement(1674..3134)
                     /locus_tag="PKM27_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08998.1"
                     /translation="MKKPILFALTFSLSFWLSGQNASDALRYSLTDPVGTARSVAVGG
                     SMGAIGAEFGGLTENPAGLGAYHFSEFVFSPALFYNQVKSSLKGSAFLQENKYGAGID
                     NLGIVIVRNGYEGSSWKKVNFAIGFNRLNDYNRNLYFEGATTGSITDHWREQALHLNP
                     DDLDDFASGLAYETGAIFDSNNDGVYESDFMDHANKAVFKSQSVESRGHQSELNFALG
                     ANYKDRLMLGASLGIPLISFREEKSYEEADPENLNPVFENLGYTEFLKTEGSGINLKL
                     GALFKFHNLFRLGLSFQSPSFLEIDETYETSASYSYLDQGQIQSYEASSPQTGIFTYK
                     IATPLRAGASFGSVLGKNGFIGASVEYVDYTQSSFSMDREFRDFERELNQDIDKQFQT
                     ALNLRAGAEIAIDVLRLRGGVFLQGSPYANENEFTTTYSIGLGVRGGRFFSDVAMRHT
                     RYDDGYLPYTTASNDPQLVNNKHQLNRLVLTLGYKF"
     gene            complement(3230..4666)
                     /locus_tag="PKM27_16890"
     CDS             complement(3230..4666)
                     /locus_tag="PKM27_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR08999.1"
                     /translation="MKKSFLRVMILALATGAFSASAQYDDIYYSSGAARSRPKPVSNQ
                     ADYYGNERYDDQEAYYEEDYDAYDDEAYNYLDEYDYSYTHRIRRFHRPLIGRGFYDPF
                     YWDPWYFDPFYYSRGFGFITPRIGIGFFSFYDFNRWNRWQRFNSYRYWDPWAFSYWSY
                     DPFLFNRYNNFYWAGNAWCPTSWYSNNYYINNNFWGNRYNGTYQPRYYTDNNSFFGPR
                     RYGTTTTGDRGPSRSSTRIFSEPTGNSTGQPLRRTTPRDVETTVVRERNVPISRNQPK
                     AAEGSSDRISPRGNNESSRATQTEKPRSFRDFDNNTQDNNQRNAPRFNDPSNQERNRN
                     MESPRRNEPISTPQSPRSDGNNGSGSSRSGSVSPRNNDGLQSRQMDMAPRNHPYLETP
                     SGSTRMRLPERETPSYSAPRTNGNSNNSIFRGSGNSGSSGNRTFNTPSRSGNSGFSGS
                     SPSSGGSRSSGGSAPQSSGGGRTSPRNQ"
     gene            4866..6332
                     /gene="proS"
                     /locus_tag="PKM27_16895"
     CDS             4866..6332
                     /gene="proS"
                     /locus_tag="PKM27_16895"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNR09000.1"
                     /translation="MAKEITARAENYAQWYLDLVQKADLADHSAVRGCMVIKPRGYAI
                     WEKIQAKLDKRFKDTGHVNAYFPLFIPKSFLSKEAEHVEGFAKECAVVTHHRLKKDPG
                     GKGLIVDPEALLEEELVVRPTSETIIWNTYRDWIQSYRDLPLLINQWANVVRWEMRTR
                     PFLRTTEFLWQEGHTAHATAEEAIEETARIHEIYARFVEDELAMPVIKGRKTASERFA
                     GAVETYTIEALMQDNKALQAGTSHFLGQNFAKAFNVTFLTEKNQNEFVWATSWGVSTR
                     MVGGMIMTHSDDDGLVLPPRMAPLQVVIIPIPKPAPALDEKTEQILKALKDKGITASY
                     DKDERNRPGFKFAQNEMYGVPVRIGMGMRDLENNQVEVVRRDTREKVMVPIDGVAGYI
                     EELLVRIQHDLFEKALKFRSDNSHYIDSYEEFKERIEEPGGFFYCHWDGTPESEARLK
                     EETKATIRCIPVDQPEEKGRCLLTGRPSSGRVIMARAY"
     gene            6509..8119
                     /locus_tag="PKM27_16900"
     CDS             6509..8119
                     /locus_tag="PKM27_16900"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR09001.1"
                     /translation="MKKILTLLLVCSFVFSYKDILHAGPGLEYTKSGGGEYNRDQIHQ
                     LILMCDTLIQQGKFDKALSLAEEAERAARKTKSVELQAMALNRQGKALMAQNKKAESR
                     FEKSNDLLIKGNIKNQGLILDNLNQLRVLATLKNDKQALSEIDYAISKLRKGQSLEQL
                     GSQAENLKAEIVNIEAEKESIEAEKKRLAEEKENLNKALTEQLSLHNSLVTDQAKLRQ
                     ESRKIQQDLEKKKNEINLMSLEQMREELIRKEQQEMIVFMQSQDSLNMERLKNQDLML
                     SESRSKRNFYIATSIALLGLFGSSFYGFIKSRQNAKLLQEKNALIEAERQRSDQLLLN
                     ILPATVASELKNNGFTKASHFEEVTVCFADFVGFTTIAGVTDPQKLIGELNTCFKAFD
                     EIIAKYDLEKIKTIGDCYMFAGGLPEEGKGNQIVEMINAAKEIQVWLDAWNEEREGLG
                     KARMDIRIGIHMGPVAAGVIGSKKFAYDIWGDTVNMAARLEQSSEKGKINISGETYEK
                     VKDIFKCEYRGKIPAKNKGLVDMYFVEN"
     gene            complement(8263..8760)
                     /locus_tag="PKM27_16905"
     CDS             complement(8263..8760)
                     /locus_tag="PKM27_16905"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNR09002.1"
                     /translation="MKKIFLIALLGMFAFESMAQAAKASPAMSTENDLIKITYGAPSK
                     KGREIFGALVPYNAVWRTGANEATEITFKKDVDFAGKMVKAGTYTLFTIPTEKEWTII
                     LNSELKQWGAYGYDKIKGKNVAEVKVPAGMASEALELFTITATAKGFDILWDKTKVSV
                     PVKGV"
     gene            complement(8871..9311)
                     /locus_tag="PKM27_16910"
     CDS             complement(8871..9311)
                     /locus_tag="PKM27_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09003.1"
                     /translation="MKQFLILFFLILASGLNGQNSSTTWNTLGKITFKKEFDAALGFK
                     IDKPIFGEEIKALSGKIVEIKGYIIPTDGYKSHTEFILSAFPYNMCFFCGGAGPETVM
                     EIYAKTPVKFTSEAVTLRGKLELNENDVNRLIYAMTEAEQVDAK"
     gene            complement(9356..10261)
                     /locus_tag="PKM27_16915"
     CDS             complement(9356..10261)
                     /locus_tag="PKM27_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR09004.1"
                     /translation="MKRREFIRNSSFLMPAAWSPDALIKSFFADPWNIKMLTRDTGIF
                     TERGGTILFHISKQGMVVVDSQFPDQAGHLISELKKRGDEPFELLINTHHHGDHSGGN
                     IAFKDLVRRVLAHKNSKANQMKVAKERNLEAGQLYPSQTFENVWSEKIGRERITLHYF
                     GPGHTDGDSLIHLEKSKIVHMGDLMFNRRHPNVDRSAGASVQNWVRVLDRAIRTFHPK
                     TTYVFGHAAEAYPVTGTRQDLEAFKSYLQALLDFTADARAKGMTKEDFLKTTEIPGAG
                     EWKGDGIARPLGAAWDELASGSQAP"
     gene            complement(10353..>11382)
                     /locus_tag="PKM27_16920"
     CDS             complement(10353..>11382)
                     /locus_tag="PKM27_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR09005.1"
                     /translation="RVPRLRALQVHNSTVYRWNRPCYGISENGKPHLRIENRVFPSGP
                     TTVDEIANAAFWLGAMTGLAADQDDIRKKVSFADISDNFVKSAQFGIDSKFNWFEDQK
                     IPAVDLILKELLPYAHSGLRSQGVDEADIDKFLGIIEERAKKHMTGARWQLRAYTKLL
                     EQTNRTEALTVLTSSMLRQQKKNLPIHKWKLPELRDLRDYKPQELLVGDFMTTDLLTA
                     QKEDIIDFVAEMMQWKRIRYTPVEDQKGRLVGLVTSRLLLQYFTDKKKFPKTVKDIMI
                     RKPVTASPSTTIMEAMSLMRTHKIGCLPVVQDNELVGIFTEMDFLGISARLMERLSEQ
                     EKMTDDEA"
BASE COUNT         2815 a         2733 c         2956 g         2878 t
ORIGIN      
        1 agggacaggc cctgatcctg ttttgggatc aggcttgatg tagtatcttc gggcatgacc
       61 ttaaaacagt tttttgattt catttctgag cacccttccc tgatcttgtt ttattcggtt
      121 gccgtgcccc tgagtgcaat ccttaccggc cttttcagca ggggagaggg acatcttgcg
      181 ccctggaaat acctttatgc tttcctggtt tattttgtct gtattccagg catttttgcc
      241 gtcacgctgg atgtctattt attcttgttt gagcggcaaa gcatctggga gtccaacctg
      301 tacacccagt ttttaccggt gctggtcatg ctgatcaccc tcatgatcat caaaaggttt
      361 gtacggctgg aatccattcc gggttttggc cggctcaccg gcctgatcct gatgatttcg
      421 gccgtcctgg tggccatgtg gtttctggac aaaacccata ttttcgtttt ctcctatatg
      481 cctttttacc aggtggtcct gttgctcgtg gttttgtttg tggtccttcg gctggcctgg
      541 cgaaaatggg cgggctgatt tttctgcccg attgattacc tttaaggtcc aattataatt
      601 tccctcgcaa aaccttactc tgcatttctt aacccaaaaa tccctttatg aaagcgttga
      661 agtatatcgg attattgctc ctcaccctgc tggccctctt cctggtcctc ggcctggtaa
      721 gcaccaaaaa agtgcacctg gaaaggacca ccctgatcaa agccccggtg agcacggtcc
      781 aggatgtggt acgccggttt tcgacccaga aagcatggaa tccctggcag gaatacgacc
      841 ccgaaatgaa agtcaccctg gaaggggagg atggaagcgt gggctcaaaa tattcctgga
      901 gcggaaataa agatgtagga aaaggctacc agatgattac caatattgcc ccggattatg
      961 tgggttcgga acttgttttt gaagaaccct ggtccagcaa ggccaccatc ggcatgaagc
     1021 tgaccgaaga ggacggcgcc acccgggcca cctggtcctt tgacagcgaa accccctttc
     1081 cctggaacgc catgaacctc tttatgaata tggataaatt gctcggcccg gattttgaaa
     1141 aaggcctgaa caaactcaag acttatattg aaaccgaggt ggataaaaaa tacaggggct
     1201 acacgataaa tgagtctgaa catccggcca ggttttttgt cgggatgcga aagaccgtgg
     1261 ctattcccca gatcacttcc ttttttgcag aacaatttgg caaaatatat gccgcgattc
     1321 aaaaagcggg cattgagatg gacgggatgc cggcgggatt atactttacc tatgatgaaa
     1381 cggcgggtac tacggacatg gtggcgggca tcccggtaaa aggcaaagcc tccgtgcccg
     1441 gattctcaac atttgaaatc ccggccggta cggaattgag cctggactat ttcggggctt
     1501 acgataaaat gggggaggcg cattatgcta tggaagacta tatcagggaa aaggcgctga
     1561 ccggcagggt tccggtgatc gaagaatatt tatccgatcc tgaactggaa ccggatactg
     1621 caaaatggct gacccgggtg atttatccgg tgtccaaata accggtttct gacctaaaat
     1681 ttgtatccca gggtcagtac cagcctgttg agctggtgtt tgttattgac cagttgggga
     1741 tcattcgaag cggtagtgta gggtaaatac ccatcatcgt agcgtgtatg gcgcatggcc
     1801 acatcggaaa agaaacgtcc tccgcggact cccaggccaa tgctgtaggt cgtggtgaat
     1861 tcgttttcat tggcataagg cgaaccctgg aggaacactc ctccccgtaa acgcaggaca
     1921 tcaatcgcga tttccgcccc ggcccgcaga tttaatgcgg tctggaattg tttatcgatg
     1981 tcctggttga gttcccgttc gaaatccctg aattcccggt ccatggaaaa actggattgg
     2041 gtataatcca cgtactccac ggaagctccg ataaatccat tcttgccgag gacggaaccg
     2101 aaactggcgc ctgcacgcag gggggtggcg attttataag tgaatattcc ggtttggggg
     2161 gaagaggctt cataagactg gatttgcccc tggtcgaggt aactgtaact ggctgaggtt
     2221 tcatacgtct cgtcaatctc aagaaatgaa ggggactgga aggatagtcc cagtcggaac
     2281 aggttgtgaa atttgaacag ggcgcccagt tttaaattta tcccgcttcc ttccgtttta
     2341 agaaactccg tgtatcccag gttttcaaat acgggattga ggttttcggg atctgcttcc
     2401 tcatagcttt tttcctcccg gaaagaaatg aggggaatcc cgagggaggc gcccagcatg
     2461 agccggtctt tataattggc cccaagggca aagttgagtt ccgattgatg gcccctggat
     2521 tcaacagact ggcttttgaa caccgctttg tttgcatggt ccataaaatc gctttcatat
     2581 accccgtcat tgttggaatc aaagatcgct ccggtctcat aggccaggcc gcttgcaaaa
     2641 tcgtcgagat cgtccgggtt gaggtgcaat gcctgttccc gccaatgatc cgtgatgctg
     2701 ccggtggtgg ctccttcaaa atagaggttg cggttgtagt cattgagacg gttgaaacca
     2761 atggcaaaat tgactttttt ccaagaggat ccttcataac cgtttcgtac gatgacgatt
     2821 cccagattgt cgatgcctgc accgtatttg ttttcctgaa gaaaagccga tcctttgagg
     2881 gaagatttta cctggttata aaacaaagcg ggagaaaaaa caaattctga aaaatgatag
     2941 gcgccaagcc cggcgggatt ttccgtaagc ccgccgaatt cggcccctat ggctcccatg
     3001 ctgcccccga cagccacgga ccgggcggtc cctaccggat cagtcaggga gtacctgagg
     3061 gcgtcgctgg cattttgacc gctgagccag aaagacaacg aaaaagtgag ggcgaacaaa
     3121 atgggttttt tcatatatcc ttattctaaa tatgttaatt ttttcacttc cgggtataaa
     3181 aaatgggtgt aatccaaagg ataacaccca tttgggtttt tttatccaat cattgattgc
     3241 gtggggaagt ccttccgcct ccgctgctct ggggtgcaga acctccactg ctccgggagc
     3301 ctccggatga tggagaagat cccgagaacc cgctgttgcc ggacctggag ggggtattaa
     3361 aagttctgtt tcccgaactt ccgctgttgc cggaaccccg gaaaatcgaa ttattggagt
     3421 tgccattggt cctgggagcg gaataacttg gtgtttccct ttccggtaaa cgcatccggg
     3481 tagacccgct tggagtttcc aggtaaggat gattacgggg tgccatgtcc atttgccggc
     3541 tctgcaggcc gtcattgttc ctggggctca cagaaccact cctgctgctc ccgcttccgt
     3601 tgtttccgtc actcctggga gattgaggag tggaaatggg ttcattcctc ctcggacttt
     3661 ccatattgcg gtttctttcc tggttggaag gatcattaaa tctaggggcg tttctttgat
     3721 tgttatcctg ggtattgttg tcaaaatccc ggaaactcct gggtttttcg gtctgcgtgg
     3781 cccggctgga ttcgttattg ccccttgggc tgatacggtc tgaagatcct tcggcagctt
     3841 tgggctggtt acggctgatg ggtacatttc tttcccttac cacggtggtt tccacatccc
     3901 tcggagtggt gcgcctcagc ggttgacccg tgctgtttcc ggtcggttcg gagaatatcc
     3961 tggtggaaga tctgctggga ccgcggtccc ccgtcgtggt cgttccgtat ctccggggcc
     4021 cgaaaaagga attgttgtcc gtataatacc tcggctgata agtcccgttg taccggttgc
     4081 cccagaaatt attgttgata taatagttat tggagtacca gctggtgggg caccaggcgt
     4141 taccagccca ataaaaattg ttgtagcggt taaaaaggaa ggggtcgtaa gaccagtagc
     4201 tgaaagccca gggatcccag tatcggtaac tgttgaaccg ttgccacctg ttccaccggt
     4261 taaaatcata aaatgagaaa aaccctatac cgatccttgg cgtgatgaat ccaaatcccc
     4321 tggaataata aaagggatca aaataccagg gatcccaata aaacgggtcg taaaacccgc
     4381 ggccgatcag cggacggtga aagcgacgga tccggtgggt ataactgtaa tcgtactcgt
     4441 ccaggtagtt ataggcttcg tcgtcatagg catcgtaatc ctcctcgtaa taggcctcct
     4501 gatcatcata acgttcgttg ccataataat cagcctgatt gctcacgggt tttggcctgc
     4561 tccgggcggc cccggaactg tagtaaatgt catcgtactg ggcggatgca gaaaacgcac
     4621 ccgtggccag cgcaaggatc attactcgga gaaaactttt tttcattttg gctttttgtt
     4681 taattgtttg agcaatttgg agtacgctta tcttttcaac atctaaatta ttaattttgt
     4741 gcctcatatt atgttaaata gaattaataa tgattgtgcg gtacgttaaa agcaagccct
     4801 gacacaatat tcattttcat ataaatttga cggattcatc agcaaaaaag ttacctgggg
     4861 gaattatggc aaaggaaatc acggcaaggg cggaaaatta tgcccaatgg tatctggacc
     4921 ttgtacagaa agcagacctg gcggaccatt ccgccgtccg gggctgcatg gtcatcaaac
     4981 ccaggggtta tgccatctgg gaaaaaatac aggccaagct ggataaacgt ttcaaagaca
     5041 ccggccatgt caacgcttac tttcccttgt ttattccgaa aagttttttg agcaaggaag
     5101 cggaacacgt ggaaggcttc gccaaagaat gcgccgtggt cacccaccat cgcctgaaaa
     5161 aagatccggg ggggaaaggc ctcatcgtag atcccgaagc cctgctggaa gaagaactgg
     5221 tggtgaggcc gacgagtgaa accatcatct ggaataccta tagagactgg atacagagtt
     5281 accgcgacct gcctttgctc atcaatcaat gggccaacgt agttcggtgg gagatgcgga
     5341 cccggccctt tttgaggacc actgaatttt tgtggcagga agggcatact gcgcatgcca
     5401 ccgctgaaga ggccatagag gagaccgcga ggatccatga gatctatgcc cgttttgttg
     5461 aagatgaact ggccatgccg gtcatcaaag ggcggaaaac ggcttcggaa cggtttgccg
     5521 gagccgtgga aacctatacc atcgaagcgc tgatgcagga caacaaagcg ctgcaggcag
     5581 gtacctctca tttcctcgga caaaattttg cgaaggcctt taatgtgacc ttcctgaccg
     5641 aaaaaaatca gaatgagttc gtctgggcaa ccagttgggg ggtgagcacc cgcatggtgg
     5701 gaggcatgat catgacccat tcggacgacg acggcctggt gttgccgccc cggatggccc
     5761 cgctccaggt ggtcatcatc cccattccca agccggctcc cgccctggac gaaaaaacgg
     5821 aacagattct caaagcttta aaggacaagg ggattaccgc ctcttacgac aaggacgagc
     5881 ggaaccgccc gggctttaaa ttcgcccaga atgaaatgta tggagttcct gtccggatag
     5941 gtatgggcat gagggacctg gaaaacaacc aggtcgaagt ggtgcgaagg gataccaggg
     6001 aaaaagtgat ggtgccgatc gatggggtgg ccggttatat tgaagagctg ttggtgcgga
     6061 ttcagcacga cttgtttgaa aaggccttga agtttaggtc ggacaacagt cattatatcg
     6121 attcctatga agaatttaag gaaaggatag aggaacccgg gggctttttt tattgccatt
     6181 gggatggaac gccggagtca gaagccaggt taaaagagga gaccaaggct accatccggt
     6241 gtataccggt cgaccagccg gaagaaaaag gccgctgcct cctgaccggc aggccgtcct
     6301 caggaagggt gatcatggcc cgggcatact gatcctgccc tttacgctaa ggcctggttt
     6361 caaagggtca aaaaataatt taactaaacc aggggttaaa tttaaggacg ggtgcccgga
     6421 cacccgttaa gccttttaca ttaaatggtg actgcggttc aataaaaacc gatttaaaga
     6481 cgtaatttta taaacagaat ttcctgacat gaaaaagata cttacactgc tgcttgtatg
     6541 ttccttcgtc ttttcatata aagatattct tcatgccggc cccgggctgg aatacacaaa
     6601 atccggcggg ggcgaataca acagggatca gattcaccag ctcatcctga tgtgtgacac
     6661 gctgatccag cagggaaaat ttgataaagc tttatccctg gcggaagaag ccgagcgtgc
     6721 ggccaggaaa accaaaagcg ttgaattgca ggccatggcc ctaaaccgtc agggcaaggc
     6781 cctgatggcc cagaataaaa aagccgaaag ccgttttgag aaaagcaacg acctgctgat
     6841 caaagggaac atcaaaaacc agggcctgat cctggataat ttaaaccagc taagggtgct
     6901 tgcgacactg aaaaatgata aacaggccct ctccgaaatc gattatgcga tttccaaact
     6961 ccggaaaggc cagtccctgg aacaattagg ttcccaggcc gaaaacctga aagcggaaat
     7021 cgtcaatatt gaagcggaga aagaaagcat agaagcggaa aaaaagagat tggcggagga
     7081 aaaggaaaac ttaaataaag ccctgacgga acagctctcc ctccacaaca gcctggtgac
     7141 cgaccaggcc aaactcaggc aggaaagccg taaaatccag caagacctgg agaagaaaaa
     7201 gaatgagatc aacctgatgt ccctggaaca gatgcgcgaa gagttgatcc gcaaagaaca
     7261 acaggaaatg attgtgttta tgcaatccca ggacagcctc aatatggaga gactgaaaaa
     7321 ccaggacctg atgttgagtg aaagccgttc caaacgcaat ttttacatcg cgacatccat
     7381 tgccctcctg ggcttgttcg gatcgagttt ttacgggttc atcaaaagcc gtcagaatgc
     7441 caaactgttg caggaaaaaa atgccctgat cgaagccgaa cgtcagcgct cggaccaact
     7501 cctgctcaac atcctgcccg ccaccgtcgc ctccgagtta aaaaacaacg ggtttaccaa
     7561 agccagccat tttgaagaag ttacggtttg ttttgccgat tttgtaggct tcaccaccat
     7621 tgccggagtg accgatcctc agaaactgat cggggaactg aacacctgct ttaaggcctt
     7681 tgatgagatc attgccaaat acgatctgga aaaaatcaaa acgatcggcg actgttatat
     7741 gtttgccgga ggccttccgg aagaagggaa aggcaaccag attgtggaga tgatcaatgc
     7801 ggcaaaagag attcaggtct ggctggatgc ctggaatgaa gaacgcgaag gccttgggaa
     7861 ggcccgcatg gacatccgga tcggcattca tatggggcct gtggccgccg gggtgatcgg
     7921 cagcaaaaag tttgcctacg atatctgggg cgatacggtc aacatggccg caaggctcga
     7981 acaatcgagc gaaaaaggca agatcaacat ttccggcgag acctacgaga aggtaaagga
     8041 tattttcaaa tgcgaatacc gcggcaagat ccccgccaaa aacaaaggcc tggtggatat
     8101 gtattttgtg gaaaactgaa tcacccctca atcctcaatc ctcacccctc aatcctcacc
     8161 cctaaaaaaa aagcgacacg atctttccat cgcatcgctt atatttttcg gttactgatt
     8221 aactagttaa ctgattaact ggctaactga ctaactgact aactacactc ctttcaccgg
     8281 aacactcacc ttggttttgt cccagagaat gtcaaaccct ttggcggtag ccgttatagt
     8341 gaataattcc agggcttctg aagccatgcc ggccggcact tttacttccg caacgttttt
     8401 acctttgatt ttatcataac cgtatgcgcc ccattgtttg agctcagaat tcaggatgat
     8461 ggtccattct ttttcagtgg ggatggtgaa cagggtgtag gtgccggctt ttaccatctt
     8521 gcctgcaaaa tccacatctt ttttgaaggt gatttcggtg gcttcgttgg ctccggtacg
     8581 ccatacggcg ttgtaaggca cgagggctcc gaagatttca cggccttttt tggagggggc
     8641 tccgtaagta atcttaatga ggtcattttc ggtgctcatc gcagggcttg ctttggcagc
     8701 ctgggccatg ctttcgaaag caaacatgcc cagcagggca atcaggaaga tttttttcat
     8761 atgaatttta gttaatgaat aattcgcaaa aatatttgat ctcaaaacca tatcaggatc
     8821 tgttttgtta taagagtatt aaaatttaag aaaactagaa cgggctgagt ttattttgca
     8881 tcgacctgct cggcttccgt catcgcataa atcaggcggt tgacatcatt ttcattcagc
     8941 tccagtttcc ccctgagcgt gacggcttcc gaggtgaatt ttaccggggt cttggcataa
     9001 atttccatga cggtttccgg tcccgcgcct ccgcaaaaga aacacatatt gtaaggaaag
     9061 gcggacagga taaattcagt atggctttta tatccgtcgg tgggaatgat atatcctttg
     9121 atttcaacga ttttgccgga cagcgctttg atctcctcac cgaaaatggg tttgtcgatc
     9181 ttaaagccca gggctgcgtc gaattccttt ttaaaagtga ttttacccaa ggtattccag
     9241 gtcgtgctgc tgttctggcc gttaagtccg gaggccagga tcaaaaagaa taaaatcagg
     9301 aattgcttca tgacgcaaaa ttagggcatt agatccattc catgccgggg aaagtttagg
     9361 gtgcctggct gccggatgcc agttcgtccc aggccgctcc cagcggacgg gcaatgccat
     9421 cccccttcca ctctcccgct ccgggtattt ccgtggtctt cagaaaatct tctttcgtca
     9481 ttcctttggc ccgggcatcc gccgtgaagt caagcaaagc ttgcaggtat gatttgaatg
     9541 cctccagatc ctgacgggta cccgtgaccg ggtaggcctc cgcggcgtgc ccgaaaacat
     9601 aggtggtttt cgggtggaag gtccggatgg cccggtccag gacccggacc cagttttgga
     9661 cactggctcc tgcggaccgg tccacattgg gatggcgccg gttgaacatc aggtccccca
     9721 tatggacaat ctttgatttt tccaggtgga tgaggctgtc tccatcggta tgccctggcc
     9781 caaaataatg cagggtgatc cgctcacggc ctattttttc actccagacg ttttcgaaag
     9841 tctgtgaagg atagagctgg cccgcttcca ggttccgctc tttggctact ttcatttgat
     9901 tggcctttga attcttatgc gccagcaccc ttcttaccag gtctttgaat gcgatattgc
     9961 ccccggaatg atccccgtgg tgatgggtat tgatcagcag ctcaaagggc tcgtcgcccc
    10021 tttttttcaa ttcagagatc aggtggcctg cctggtccgg aaactgagaa tccacgacca
    10081 ccatgccctg tttggaaata tggaacagga tcgtgccgcc cctttccgta aatattccgg
    10141 tgtcccgggt gagcattttt atattccagg gatctgcaaa aaaacttttg atgagggcat
    10201 ccggagacca tgctgcaggc atcaggaacg aggagttgcg gatgaattcg cgtcttttca
    10261 tggtgtataa aatgatttac aaaagtagtg gaaatagcca gggtttgccg ggcgcttccg
    10321 aggggcaccg ggagtgctgt ccggattctt attcaggctt catcatcggt catcttctcc
    10381 tgttcgctga gccgctccat cagtcttgcc gaaatgccaa ggaagtccat ttcagtgaaa
    10441 atccccacca gttcgttgtc ctggactacc ggaaggcagc ctattttatg ggttcgcatc
    10501 aggctcatgg cttccatgat ggtggtggaa gggctggcgg tcaccggctt gcggatcatg
    10561 atatccttta ccgtctttgg aaatttcttt ttatccgtaa aatactgaag cagcaggcgt
    10621 gaggtcacga ggccaacaag gcggcctttc tggtcctcga ccggcgtata acggatccgc
    10681 ttccactgca tcatttcagc cacaaaatcg atgatgtcct ccttttgggc cgtgagcagg
    10741 tcggtcgtca taaaatcccc caccagcaat tcctggggtt tgtaatccct gaggtcgcgg
    10801 agttccggca atttccattt gtggatcgga aggttttttt tctgttgcct gagcatggaa
    10861 gaagtcagca ccgtaagggc ttccgtgcgg ttggtttgtt ccagcagttt ggtgtaggcc
    10921 cggagttgcc agcgcgcgcc ggtcatatgt tttttagccc tttcttcgat aatgcccagg
    10981 aatttgtcaa tatcggcttc atccacgccc tgggaccgca gcccgctgtg ggcatagggc
    11041 aggagttctt tcaggatcag gtcgacggca gggatcttct ggtcttcaaa ccagttgaat
    11101 ttggagtcga tgccaaactg tgcgctctta acaaaattgt ctgaaatgtc cgcaaaggag
    11161 actttcttgc gaatgtcgtc ttgatctgct gcgagcccgg tcatggctcc cagccaaaag
    11221 gccgcattgg cgatttcatc tacggtggtg ggtccgcttg ggaatacccg gttttcaatt
    11281 ctgaggtggg gcttgccgtt ttcactgatc ccgtaacagg gccggttcca gcggtaaacc
    11341 gtggaattgt gcacctgcaa ggcccggagc ctgggcacgc gg
//
LOCUS       DAOQXT010000128        11303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6196_length_11303_cov_5.963105, whole genome shotgun sequence.
ACCESSION   DAOQXT010000128 DAOQXT010000000
VERSION     DAOQXT010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6196_length_11303_cov_5.963105"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PKM27_16925"
     CDS             complement(<1..1043)
                     /locus_tag="PKM27_16925"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR09006.1"
                     /translation="MTFGKVLDKYRKYSFSEQDKGERFERLMKAYLLTDPKYAYKFKK
                     VWLWSEFPGKVDLGGWDTGIDLVALTIEGDYWAIQCKCYSESAIIGKPEVDSFLATSS
                     RKFKGEDLKTTGFAHRLWISTTNNWGTNAQEAIKNQHPPVSRINLFDLTSAPVDWEKL
                     EANISGEDARSTRKSLRPHQILALTKTHEHFKTRARGKLIMACGTGKTFNSLRIAEHE
                     TNGNGFILFLVPSISLLGQTLNEWSADALEPINAICICSDPEVSKKRTQLEDIDTSSV
                     IDLAYPASTNVKDILHQFQYLDKKRNKGLTVVFSTYQSIEVIAKAQKQLQKEQPEFGE
                     FDLIICDEAHRTTG"
     gene            complement(1997..2461)
                     /locus_tag="PKM27_16930"
     CDS             complement(1997..2461)
                     /locus_tag="PKM27_16930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HNR09007.1"
                     /translation="MRTTKPVPAKLKVLRGTYRKDRDGKGLKVPPGQEIPEPPDRFNK
                     DQKALWQTVCTTLRSVDLLQPVGIPLLIIYCDQSRLYQEAFDEVKRSGMITTTVTKYG
                     TVMKKNPALEVMNGAIKIMLAISDQFGFTPLAQSKIKGVARQEQEYNDGYDF"
     gene            complement(2574..3095)
                     /locus_tag="PKM27_16935"
     CDS             complement(2574..3095)
                     /locus_tag="PKM27_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09008.1"
                     /translation="MKIHLPSLPRVEYPPGFKEIWQKYRERAEQMPPHIFTGQPPAGP
                     LRPENSGIPEPEPETTPTITDLERLRRTQAIINKGIEDRIMQAEVDQDSLSPPLPCID
                     DWQDMERYFTSATIPPEPFRIGCVTVNDPIEAIRYHLSGLKAAPPESLVAKADVYHLR
                     VIKAALEALEPPP"
     gene            complement(3163..4290)
                     /locus_tag="PKM27_16940"
     CDS             complement(3163..4290)
                     /locus_tag="PKM27_16940"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR09009.1"
                     /translation="MSHYPDSELHKELPQDNGIPGNPEARKAEAARLQAGIATLENEY
                     KSKHPETTEPPRIEKHIGLFTINTASGWVTEAMNRSVPGMLFDTFWYEGELCILYATT
                     GVGKSVLAVQIADSISRGLAIPGFELGVTEQPVLYIDYELSDIQFAKRYVNAATGSKY
                     DFSPNLYRLEINPEADIASGFLEMEFMNSIKQAVTQTNAKIVIVDNLTYLKSGTEKAA
                     EALPLMKTLNSLKKKLKLSILCLAHTPKRDKSRPITENDLQGSKMLSNHCDCLFAIGE
                     SSRDSGMRYLKQIKTRNTEKVYDWDNVVLCTLEKPDNFLGFRFLDYGTEGEHLRVEMD
                     ADRDELKQKIKKMAELKMTQRDISKELGISLGKVNKMLKPD"
     gene            complement(4287..4640)
                     /locus_tag="PKM27_16945"
     CDS             complement(4287..4640)
                     /locus_tag="PKM27_16945"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR09010.1"
                     /translation="MSTILSPNVILSTIDPDYLIETISTRVTEKLSLYLPATANDRQT
                     DPKKPLRHSAAASYLGIATQTLYQYISEGKIKPHKPGKHNLFYKDDLDAFIRGEQPVQ
                     PDGSQFLKRNKKARI"
     gene            complement(4706..5590)
                     /locus_tag="PKM27_16950"
     CDS             complement(4706..5590)
                     /locus_tag="PKM27_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09011.1"
                     /translation="METDKKARLIFEIPKVDGYFVNPNFFKSNELIKTNINRPTVEEA
                     HWTVIHNGKKYKVFVAELYAILSEMAIPLPDGNTFNGRKFIKDYATGFNKGTDYFNSE
                     FALKIDTLYSSGSSGYVDNLKSCYFKKAPDIQHGWIYWASTYPLIINPTVIEEYGFYA
                     GIINAVNSLRIKHQTLFKDFESNESKSNIKEGPKPRGKAFAIHYVLAYIYDCIVTGVA
                     LPVASEKKRLEQICADREIDLAGDTFYRKYREARRIDLNSKKALTKKAGKNWGEMVIY
                     LSKNPDEVYKHLKDKKLV"
     gene            complement(5614..6876)
                     /locus_tag="PKM27_16955"
     CDS             complement(5614..6876)
                     /locus_tag="PKM27_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNR09012.1"
                     /translation="MNESRPKVYLTLDRRRPKNDGTFPVKLYIYDPATTRSKRYSTTL
                     SFAPEEFKSIWQTTRPRATYLAIREDLRAIISKAEKDLEGIHPFSFEAYEAKLFRPSA
                     EGQNALWHYDERIRSLKELKQIGTASWYKCSKKSLQQFLEHQKDRNIDRLNFDKITPK
                     WLRQYDFFMQESGKSKTTIAMYLRALRAVFVDAIKSGYIDRSQHPFGEGKYELKEGGR
                     VKKALSREELKLLYISEPANPEQQKAKDFWFFSYLCNGMNVKDIINFRYGQIGKDSFV
                     FERIKTKNTNRQSQPIQVFLNEHVRYVIQEYGNQSTGRPNQLVFPILSDTDSAERQHM
                     KTQAFTRFINQHIKKLAMANGITGEISTYFARHSFATGLIRNGKPIAFVQEALGHASS
                     KTTQNYIAGFLNDEKKEAAEATYQDLIS"
     gene            7257..7931
                     /locus_tag="PKM27_16960"
     CDS             7257..7931
                     /locus_tag="PKM27_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09013.1"
                     /translation="MYPQKTETFSACFRQFREIDPQAYHTIIRFFEEHAFIIKSLDFE
                     EYFEIMTAYVRSLFEAGAYQKHADMSDVIIEAAIINNLKVYRGEDIYEKTLFQKAASL
                     YHLQEFTKSDHILSELIKMNPGENTYRLFLIKNRLQVKPAYLHVLQGLSIFLYFLSIL
                     IISVQTFYIDPFRPEWTSLAFSARNTTFITASVLLILSDLVFRGVTWYGVHSLASEAR
                     EKLKRS"
     gene            7992..>11303
                     /gene="secA"
                     /locus_tag="PKM27_16965"
     CDS             7992..>11303
                     /gene="secA"
                     /locus_tag="PKM27_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNR09014.1"
                     /translation="MFDLLKSGLKKIFGGSKQDKDLARYQPLVDQALAYYSTLQEISN
                     DDLRDHTRRFRQTIEEGLAEINAQINETRQQANEEADPEQKEELFKEVDRLGKERDKL
                     LEEILLELLPQAFAVVKETCRRFTQNNQIRVTATEHDRNIAAQKAYVTIEGDEALWAN
                     EWVAAGGAIKWNMVPYDVQLIGATALHEGKIAEMATGEGKTLVSVLPAYLNGLSGLGV
                     HIVTVNDYLARRDSEWVGPIHEFLQLTVDCIDKYQPNSTARKKAYACDIIYGTNSEFG
                     FDYLRDNMVVSPDELVQGKHHFAMVDEVDSVLIDDARTPLIISGPASQGQEEQEYLDL
                     KPKVERVIEAQKRLATQFLTEARRLIAEGKTGNEEGEGGLSLFRSYRALPKNRALIKF
                     LSEEGMKVILQKTENYYMQEQSKNMHKADAPLYFTIDEKNRNVELTDKGGEYLSQGNE
                     DPGFFILPDIATSLTDIEKDADLDEAGRRESKEKIIQDYAIKSRRLHSVNQLLKAYTL
                     FERDEEYVVMDGQVKIVDEQTGRMMEGRRYSDGLHQAIEAKENVKVGDITQTYATVTL
                     QNYFRMYHKLAGMTGTAETEAKELWDIYKLDVVVIPTNKPCVRDDREDLVFKTAREKF
                     NAVIDEIVALSTAGRPVLVGTTSVEISEKLSRMLQLRGIKHNVLNAKQHQREAEIVAE
                     AGKRGNVTIATNMAGRGTDIKISDEIKALGGLAIVGTERHESRRVDRQLRGRAGRQGD
                     PGTSQFYVSLEDSLMRLFATERMAAWMDRLGHKEGEVIQHSMVSKSIERAQKKVEENN
                     FGIRKRLLEYDDVMNIQREAIYTKRRNALSGERLAVDLNRIFLTVAESLVESYKSAGD
                     FDGFREESMRTLGLDPEMDAADFKEGNAGEVLDRYLGMVRGKYDRKKEQITEVLLPII
                     RQVHQREGQRYRRISIPFTDGRSRMLPISADIELAVKTNGKSIMLDIEKAVNLALIDD
                     SWKEHLRNMDELKDSVQGASFEQKDPLVIYKMEAYGLFEGLVGKVNREVASYLMDGQL
                     MIQADRAPQEAKERKTDLSRVSTNRAAEEEQNRRRAAMQSAGEPQKVETIRRADKKIG
                     RNDPCPCGSGKKYKKC"
BASE COUNT         2795 a         2720 c         2690 g         3098 t
ORIGIN      
        1 cccgtggtac ggtgggcttc atcgcatata atcaaatcaa attcgccaaa ctccggctgc
       61 tctttttgta attgcttttg agctttggca attacctcaa tactttggta agtgctgaaa
      121 actaccgtta agcctttatt gcgtttctta tctaaatatt ggaattggtg cagtatgtct
      181 tttacattgg tggaggcggg ataggctaag tcaattacgg aggaagtatc aatatcctct
      241 aattgcgttc gcttttttga tacttccggg tcgctgcaaa tacatatagc attaataggc
      301 tctaaggcat cggcgctcca ttcgtttaac gtctgtccta ataaagaaat agagggcact
      361 aaaaataata taaagccatt cccgttggtt tcgtgttcgg ctatgcggag gctgttgaat
      421 gttttgcctg ttccgcaagc cattattaat ttgcccctgg cacgggtttt aaagtgttcg
      481 tgcgttttcg tgagggctaa gatttggtgc ggcctcaaac ttttacgggt ggatcgggca
      541 tcttccccgc ttatattggc ctccaatttt tcccaatcaa ccggggcgct ggtcagatca
      601 aagaggttaa ttcttgatac cggggggtgc tggttcttta tggcctcctg agcgttcgtc
      661 ccccagttgt ttgtggtgga aatccaaagg cggtgggcaa aacctgttgt tttcaaatcc
      721 tcccctttga acttgcggct gctggtggcc aggaatgaat ctacctccgg tttgccaatt
      781 atagcgctct ctgaataaca tttacattgt atagcccaat agtcgccctc aatggtcagg
      841 gctactaagt caatgccggt gtcccagcct cccaaatcaa cctttccggg aaactcactc
      901 catagccaaa cctttttgaa tttataggca tatttggggt ctgtaagcag gtaggctttc
      961 attaagcgct caaacctttc tcctttgtct tgttctgaaa aggaatattt gcggtatttg
     1021 tctaatactt tgccgaaagt catagctcta attcaatttt ttcggaggtt tcccgtttgc
     1081 gctggtcaat tattttggca taaatcttcc cggtgtccta gcattttcga tacaatttgt
     1141 taaatgtaag caactgttgc atttaaagcc tttggtaagt caaaggttct taattttaag
     1201 atttttacat acacgaatat attaaaaatt actttctacc tcccagaaga gcatatcaat
     1261 gctctttcaa gttctttgta acttatgctt tgggcttttt gtatttgctg cataagtttc
     1321 aaatactttt ttgtagcctt gcccgcatac tgttttttaa actgcttttt gtaaagttgt
     1381 tcaaaaagtt tgtcggtccg gaaatataca cccagcgttt tatctaaatc tctatacttt
     1441 ttactttgtg tttgcttttc gtacatacag cccctgaacg ctgaacggtg ataaaaatag
     1501 gtgttggcta aatataactt tcggcaacgc ttaccagtat aagggcatac gaaataccaa
     1561 acaacccctt tacctaaatt ggaaggggct gtaacaagaa atgctgacag tttttaggtc
     1621 atcatacaag gtcgggaaag agttcggctt tggtatctta aatttatttt ggctttcaat
     1681 gaatagagaa acatttgtta ccgtgctgta tgggttcagc tttacgggca atatcctgtt
     1741 cccagctttc aggctttgac tgttcgggct tattgaaata gtaaacgggt tcgagctgct
     1801 ccaacacttt ctcctcaacc catgcttgaa cggctggggt tggttcggta gcttcgagtt
     1861 taggtaaggc aaaatccttg taattgaact ttattaaata gtcggcaagg tcaaatcttt
     1921 agctccatat tgaaaataaa gcataagaaa aatttgcaac tggctggggg atagttttgg
     1981 actaaaatcg gccaatttaa aaatcataac catcattata ctcctgttcc tgcctggcca
     2041 cccccttgat cttgctttgt gccaatgggg taaaaccgaa ttggtcggaa attgcgagca
     2101 tgatcttaat agctccattc atcacctcca gagccggatt ttttttcatg accgttccat
     2161 atttggttac tgtggttgtg atcattccac ttctctttac ttcgtcaaag gcttcctggt
     2221 atagcctaga ttggtcgcaa taaataatta acagcgggat tccgaccggc tgcaacaaat
     2281 ccacggacct taaggtggtg caaacggtct gccagagggc tttctgatcc ttattaaagc
     2341 ggtcgggtgg ttccgggatc tcctggccgg gtggtacttt taatcctttt ccgtcccggt
     2401 ctttgcggta tgtgcccctt aaaactttca gtttcgcggg cacgggtttt gtagttcgca
     2461 ttgagtgtgc ttttaaatga taaaattaca agtttaaact tgacagcgca cgtgtacgat
     2521 cccaagcgtg ctcttcgggg acggttcatc caaggaatga atggcccctc cccctatgga
     2581 ggcggttcca gggcttccag ggcagctttt attaccctaa gatggtaaac gtccgcttta
     2641 gctaccaggc tttctggtgg tgctgccttt aagcctgaaa gatggtatcg gatggcttca
     2701 attgggtcat tcacggtaac gcagccaatt ctgaacggtt cgggtgggat ggttgcggat
     2761 gtgaaatacc gctccatgtc ctgccagtcg tcaatacagg ggaggggggg tgacaggctg
     2821 tcctggtcaa cttcggcctg catgatccgg tcttcgatgc ccttgttaat gatcgcctgg
     2881 gtacgtcgga ggcgttccaa gtcggtgatg gtgggtgtgg tttcgggttc tggttcagga
     2941 atcccggagt tctccggccg gaggggacca gccggaggtt gtccggtaaa tatgtgcggt
     3001 ggcatctgtt ctgcccgctc ccggtatttc tgccagattt ccttaaaacc tggtggatac
     3061 tcaacgcgcg gaagtgaagg aaggtggatt ttcatagtgt gtgttcattt tgttcatttt
     3121 gttcatggca tgaacagcgt gaacagcgtg aacaggaata ttttagtcgg gttttagcat
     3181 tttattcacc ttgcccaaac tgatacccaa ctccttgcta atatcacgct gggtcatttt
     3241 cagctcagcc attttcttaa ttttttgctt cagctcatcc ctgtcggcat ccatttctac
     3301 ccggagatgt tcgccctctg ttccataatc caagaatcga aagcccaaaa aattgtccgg
     3361 tttctcaaga gtgcagagta ccacgttatc ccagtcgtaa accttctctg tgtttctggt
     3421 tttgatctgt tttaaatatc tcatccccga gtcgcgagaa ctttcaccta tcgcaaataa
     3481 acaatcgcaa tgattggata gcatcttgga tccttgtaaa tcattttcgg tgatcggtct
     3541 cgatttatcg cgttttggcg tgtgggcgag gcaaaggatt gatagtttaa gttttttctt
     3601 taaagagttt agcgtcttca ttaatggcag ggcttcggct gccttttccg tcccggattt
     3661 caaataggta aggttgtcga caatcacaat ttttgcattg gtttgcgtta cagcctgctt
     3721 aatggaattc atgaattcca tttcgagaaa cccggatgcg atgtctgctt ctgggtttat
     3781 ttctaatcga tatagattag ggctgaaatc gtatttgctc ccggttgccg catttacgta
     3841 tctcttggcg aattgtatat ctgagagttc gtaatctata tacaatacag gctgctcggt
     3901 aactccaagt tcaaatcctg ggatcgcaag ccctcgactt attgagtctg caatttggac
     3961 agcaaggaca gatttaccaa ctccagtagt tgcatataaa atgcataatt caccctcata
     4021 ccaaaatgtg tcaaacagca ttccagggac cgatcggttc attgcttcag tcacccatcc
     4081 ggatgcggtg ttgatcgtaa atagcccgat gtgtttttcg attcgcggcg gttcggtcgt
     4141 ctcaggatgc ttagatttat actcattttc cagagtggcg atgcccgctt gaagtcgggc
     4201 tgcttcggcc ttgcgcgctt caggatttcc cggaattccg ttatcttgcg ggagttcttt
     4261 atgcagctcg gaatctgggt aatgactcat atccgggctt ttttattgcg tttcaaaaat
     4321 tggcttccgt ctggttgtac cggctgctct cctcgaatga acgcatcgag atcatcctta
     4381 tagaataaat tgtgcttgcc gggtttgtgc ggcttgatct tgccctcgct gatgtactgg
     4441 tacaacgttt gagtggctat tccaagatag gatgcggccg cagagtgccg gagtggcttt
     4501 ttgggatcgg tttgtcgatc atttgccgtc gctggaaggt atagagaaag tttttccgtg
     4561 acgcgggtag aaattgtttc gatcagatag tctggatcga ttgtgcttag aatgacgttt
     4621 gggcttagaa ttgtagacat aattttaaaa atttatgtaa caatcctacg cggggaattt
     4681 tggggatttt gtcctttttt tgcccctata ccagcttctt gtctttcaag tgtttatata
     4741 cttcatcggg atttttagaa agatatatta ccatttctcc ccaattcttc cccgcttttt
     4801 ttgtgagggc ttttttagaa tttaagtcga tgcgtcttgc ttctctgtat tttctataaa
     4861 atgtatcccc ggcaaggtct atttccctgt cagcgcatat ttgctccaac cttttctttt
     4921 cgcttgctac tggtaaagca actccggtca ctatgcaatc ataaatgtag gccaatacat
     4981 agtgtattgc aaatgctttt ccccgtggtt ttgggccttc cttaatattc gactttgatt
     5041 cattggattc aaaatcttta aataatgtct gatgcttaat ccttaggcta ttcacagcat
     5101 taatgatgcc agcgtaaaat ccgtattcct caattactgt tggattaata atcaaaggat
     5161 atgtacttgc ccagtaaatc catccatgtt gaatatcagg tgccttttta aaataacaag
     5221 atttcaaatt atccacatat ccggatgatc cactactata gagggtgtct atttttaaag
     5281 caaattcaga attaaaataa tctgtcccct tattaaatcc agttgcatag tctttaataa
     5341 attttcttcc attgaatgtg tttccgtccg gtaatggaat agccatttca ctaagaattg
     5401 catacagttc ggctacaaag accttatatt ttttgccatt atgaattact gtccaatggg
     5461 cttcttctac tgttggcctg tttatgttgg ttttaattag ctcattgctt ttgaaaaaat
     5521 ttgggttgac aaagtaacca tctactttag gtatttcaaa tattagcctt gccttcttgt
     5581 cggtttccat gtactttatg ctttccccta aagttaagaa atcaaatcct ggtaagtggc
     5641 ttcggccgct tccttctttt catcattgag aaaaccggcg atatagtttt gggtggtctt
     5701 gctggaggca tggcccaggg cctcctgaac aaatgcaatg ggtttgccgt tgcggattaa
     5761 tccggtggcg aaactgtgcc gggcaaaata ggtgcttatc tctccggtga tcccgttggc
     5821 cattgccagt tttttgatgt gctggttgat gaaccgggtg aatgcctggg tcttcatgtg
     5881 ttgccgctcc gcgctgtcag tatccgaaag aatagggaaa accagttgat tgggacggcc
     5941 ggttgactgg ttgccgtatt cctgaattac ataccggacg tgttcattaa gaaatacctg
     6001 gatcggctgg ctctgcctgt tggtgttctt ggtcttgatt ctttcaaata caaaactgtc
     6061 tttgccgatc tgtccatatc ggaaattgat aatatccttt acattcattc cattgcagag
     6121 gtaggagaaa aaccaaaagt cttttgcctt ttgctgttcc gggttggcgg gttcgctgat
     6181 gtataacagt ttcagttcct cccttgaaag cgccttttta accctccccc cctctttcag
     6241 ttcgtatttc ccctccccga aaggatgctg ggatcggtca atatatcccg acttgatcgc
     6301 gtcaacgaag acagcgcgga gggcccttag atacatggca atcgtggttt ttgatttgcc
     6361 ggactcttgc atgaaaaagt cgtattgccg cagccattta ggcgtgatct tgtcaaagtt
     6421 caacctgtcg atattccggt ccttctgatg ctccaggaac tgttgcaggg atttttttga
     6481 acatttatac caggatgcgg tgcctatttg ttttagctct ttcaggctcc ggatgcgttc
     6541 gtcgtagtgc cacaatgcgt tttgcccctc cgctgatggc ctgaataatt ttgcctcata
     6601 ggcttcaaaa ctgaatgggt gaattccttc caggtctttt tcggcttttg aaataatggc
     6661 cctcagatcc tccctgatgg cgaggtaggt cgccctgggc ctggtagtct gccaaatact
     6721 tttaaactct tctggggcaa aggaaagggt tgtagaatac cgttttgatc gggttgtagc
     6781 tggatcgtag atataaagtt taacgggaaa tgtaccgtca ttttttggcc tcctcctgtc
     6841 taaagtaagg tagaccttcg gccgggattc gttcatgtcc tttatgcttt tttggcaaat
     6901 tacggagctt cttgacgatt tgccaaaaaa tttgccaaaa agtcgataga tattcaataa
     6961 atatcaaaag cctggagata ctgtaaaatt aggcacttcc agcgtggcag taaggaaacc
     7021 gagataaaat actgaagatc aaaaatgcaa aaattctgcc ttacaagcag agggtcggcg
     7081 gttcgatccc gtcaactccc actgaagcct cgatccttct gattcggggc tttttttttg
     7141 aggataaggt cggtgtcctg ttgcgtgatt cgcggagatg ttaaatttca tcctaatcat
     7201 gcaaccttct aatttccctt gaaccatttt cccaaacgac ttaattttat caggtcgtgt
     7261 atccgcaaaa aacagagacc ttctccgcct gcttcaggca gttcagggaa atagaccccc
     7321 aggcctatca taccatcatc cggttttttg aagagcacgc cttcatcatc aaatccctgg
     7381 actttgaaga gtattttgag atcatgacgg cctatgtccg gtccctgttc gaggccgggg
     7441 cctaccagaa acacgccgac atgtcggatg tgatcatcga agccgccatc atcaacaacc
     7501 tgaaggtcta ccggggcgag gacatttatg aaaaaaccct gttccagaaa gcggcctccc
     7561 tatatcatct ccaggaattt accaaaagcg accacatcct ttccgaactg atcaaaatga
     7621 accccggcga aaatacctat cgcctcttcc tgatcaaaaa ccggctgcag gtcaaaccgg
     7681 cttacctgca cgtgctccag gggctgtcca tcttcctgta cttcctgtcg atcctcatca
     7741 tttcagtaca aaccttttat atcgatccct tccggcccga atggacgagc ctggccttct
     7801 ccgcccgcaa tacgaccttc attaccgcct cggtcctgct catcctttcc gaccttgttt
     7861 tccggggggt gacctggtac ggagtccata gtctcgccag tgaggcgaga gaaaaactaa
     7921 agaggtcctg agcgggtttt acactcccct atttcctgta cctttgcccg gcttagcata
     7981 cacattccac tatgtttgat ttattgaaaa gcggcctcaa aaaaattttc ggaggctcaa
     8041 aacaggataa ggacctggcc agatatcaac cccttgtaga ccaggcgctt gcctactact
     8101 ccaccctgca ggaaatctcc aacgacgact tgagggacca tacccgccgg ttccgccaaa
     8161 ccatcgaaga agggctggct gagatcaacg cccagatcaa cgaaacccgc caacaggcca
     8221 acgaggaagc cgaccccgaa caaaaagaag aactgtttaa agaagtcgac cggctcggga
     8281 aagaaaggga caaactcctg gaagagatcc ttctggaact cctcccccag gctttcgccg
     8341 tggtcaaaga aacctgccgg cgttttaccc aaaacaacca aatccgggtg accgccaccg
     8401 aacacgaccg gaacatcgcc gcccaaaaag cttatgtcac gatcgaaggc gacgaggctc
     8461 tctgggccaa tgaatgggtc gccgccggag gggccatcaa atggaatatg gtgccctacg
     8521 acgtccagct catcggggcc accgccttgc atgaaggcaa aatcgcggaa atggccaccg
     8581 gggaaggaaa aaccctggtc tccgtcctgc ccgcctatct caatggcctc agcggtctgg
     8641 gagtacatat cgtgacggtc aacgactacc tggcgcgccg ggacagcgaa tgggtcggac
     8701 ccatccacga attcctccag ttaaccgtgg attgcatcga caaataccag cccaacagca
     8761 cggcaaggaa aaaagcttat gcctgcgata tcatatacgg taccaacagc gaattcggtt
     8821 tcgattacct ccgggataat atggtggtct ccccggatga actcgtccag ggcaagcacc
     8881 attttgccat ggtggacgaa gtggattcgg tcctgatcga cgacgccagg acccccctca
     8941 tcatatccgg ccccgcctcc cagggtcagg aagaacagga atacctcgac ctcaaaccaa
     9001 aggtggagcg ggtcatcgaa gcccaaaaga gactggccac ccagtttctg accgaagcgc
     9061 gccggctgat tgccgaaggg aagacaggca atgaagaagg cgaaggagga ctgtccctct
     9121 ttcgttccta ccgcgccctg cccaaaaaca gggccctgat caaattcctc agcgaagaag
     9181 gcatgaaggt gatccttcaa aaaaccgaaa actattacat gcaggagcag tccaaaaaca
     9241 tgcataaggc ggatgctcct ctgtacttta ccattgatga aaaaaaccgc aatgttgaac
     9301 tgaccgacaa gggcggggaa tatctttccc agggcaacga ggaccctggt ttttttatcc
     9361 ttcccgatat tgccaccagt cttaccgaca tcgaaaagga tgcggacctg gatgaggccg
     9421 gccgcaggga gtccaaagaa aaaatcatcc aggattacgc catcaaatcc agacgcctgc
     9481 attccgtcaa ccagttgctc aaagcctata ccctttttga acgggacgag gaatacgtcg
     9541 tgatggacgg ccaggtgaaa atcgtggatg aacaaaccgg gcgtatgatg gagggccgcc
     9601 gctattcgga cgggctccac caggccatcg aagccaagga aaacgtaaaa gtgggcgaca
     9661 tcacccagac ctatgcgacc gtcaccctcc agaattattt caggatgtac cacaagctcg
     9721 ccgggatgac gggtaccgcc gagacggagg ccaaagaact ctgggacatc tacaagctgg
     9781 atgtggtcgt gatcccgacc aataaaccct gcgtgcggga tgaccgggag gacctggtct
     9841 ttaaaaccgc gagggaaaaa ttcaacgcgg tcattgatga gattgtcgct ttatccaccg
     9901 cgggaaggcc cgtgctggtg ggcaccactt ccgtggagat ctccgaaaaa ctgagccgca
     9961 tgctgcagct ccggggcatc aaacacaatg tgctcaacgc caaacagcac cagcgggaag
    10021 ccgaaatcgt ggccgaagcc gggaagaggg gaaatgtcac cattgccacc aacatggccg
    10081 gccggggaac ggacatcaag atctccgacg aaatcaaggc cctgggcggt ctcgccatcg
    10141 tgggcaccga gcggcacgaa tccaggcggg tggaccgtca gttgcgcgga cgggcgggac
    10201 ggcagggcga ccccggaacc tcccagtttt acgtctccct cgaagacagc ctgatgcgac
    10261 tttttgccac ggaaagaatg gccgcctgga tggaccgcct gggccacaag gaaggcgaag
    10321 tgatccagca cagcatggta tccaaatcca tcgaaagggc acagaaaaaa gtggaagaaa
    10381 ataatttcgg catccgcaag cggttgctgg aatacgatga cgtgatgaat atccagcggg
    10441 aagccatcta caccaaacgc aggaacgcct tatccggtga gcggctggcc gttgacctca
    10501 accgcatctt cctgaccgtc gccgaaagtc tggtggaatc ctataaaagc gccggggatt
    10561 ttgacggttt ccgcgaggaa tccatgcgca ccctgggcct ggaccccgaa atggacgccg
    10621 ccgattttaa ggaaggaaat gccggagaag tcctggacag gtacctgggc atggtgaggg
    10681 gcaaatacga ccgcaaaaag gaacagatca cagaagtcct gttgcccatc atccgccagg
    10741 tgcaccagcg cgaagggcag cggtaccgaa ggatctccat tcccttcacc gacggccgct
    10801 ccaggatgct cccgatctcc gccgacatcg agctggcggt caaaaccaat ggcaagagca
    10861 tcatgctgga cattgaaaaa gcggtcaacc tcgcgctgat cgatgacagc tggaaagaac
    10921 atctccgtaa tatggacgaa ctgaaagatt ccgtccaggg agccagtttc gaacaaaaag
    10981 acccgctggt catctataaa atggaagctt atggtttgtt tgagggactg gtaggcaagg
    11041 tcaaccggga agtggcttct tacctcatgg acggacagct catgatccag gcagaccggg
    11101 cgccccagga ggccaaggaa aggaaaaccg atctttccag ggtgtccacc aaccgggcag
    11161 ccgaagagga acaaaaccgg cgccgggccg ccatgcaatc cgcaggagag ccccagaaag
    11221 tggaaaccat acgccgggcg gacaaaaaga tcggacgaaa cgatccctgt ccctgcggca
    11281 gcgggaagaa atataagaaa tgc
//
LOCUS       DAOQXT010000129        11221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6240_length_11221_cov_5.457997, whole genome shotgun sequence.
ACCESSION   DAOQXT010000129 DAOQXT010000000
VERSION     DAOQXT010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11221
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6240_length_11221_cov_5.457997"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1104
                     /locus_tag="PKM27_16970"
     CDS             172..1104
                     /locus_tag="PKM27_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNR09015.1"
                     /translation="MRRLEEPGIREISKLELLANQVVEGFIVGLHQSPFHGFSVEFAE
                     HRIYNEGESTKNIDWKVFARTDRLYSKRFEEETNLRCQIVVDASSSMQFPKAASENPL
                     DSQNKFVFSALGAASLMSLLKRQRDAFGLDLFDETLKIHTEAKSSTAHYRLLLSHLEK
                     VLNDPGQHKGTRTAQVLHEIADRIHKRSLVIIFSDMMDDPSHIREVFSSLQHLKHNKH
                     EVILFHTVDHNKEIEFQYENRPHLFIDLETGEKVRLLPREVEKEYRRQMAALYDQIRM
                     TCLQYKIDLVEADIAKGYKPILQAYLAKRSRMAV"
     gene            1154..1933
                     /locus_tag="PKM27_16975"
     CDS             1154..1933
                     /locus_tag="PKM27_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HNR09016.1"
                     /translation="MHKLKLISTKAPKGMDKQATKTLLKHMTKEIGDYQHALYAEKKQ
                     SLLVIFQGVDASGKDGSTKKVFEECGTIGIDAFGFKKPTEEEFAHDFLWRVHQRAPAK
                     GYIQIYNRSHYEDIIIQRVHKWIDLKRVKQRMDAINAFEQLLEFDNNTKVLKFYMHIS
                     KEKQRIKLQERIDDPARNWKHNSVDWKEALHWDEYMKAYEYAINNSVIPWHVVPVDQK
                     WYRNFYIAKIVLQTLKKMNPQLPLLSAEDKRVMAEIQANLK"
     gene            1936..2352
                     /locus_tag="PKM27_16980"
     CDS             1936..2352
                     /locus_tag="PKM27_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNR09017.1"
                     /translation="MDQVRADKWLWSIRLFKTRTVAADACKEGRVYRGEQALKASSEI
                     KAGDLLRVHKDGFRLMIRVKDLLEKRVSAQLAREYYENLTPEEELNKYKSWFIGKASA
                     EVREKGAGRPTKKERREIDGYKDTAFDQEIEPWLEE"
     gene            2349..3296
                     /locus_tag="PKM27_16985"
     CDS             2349..3296
                     /locus_tag="PKM27_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09018.1"
                     /translation="MNSNLPYSVRFLSLFFIWCRPVSATAQEDPAAIYRHIHLDSVVI
                     TAERGGFDVLDFIELVLRDTSFYEAFRNLRFASYSFENKLVILDKSRQEKAGYRSLAH
                     QVYDSSCWQMKEEQRSVSGPFLDPKGDYRFYTAKLYDRLFFTHGKICHPAPADVQDTR
                     SKGMEKHVEELKTLIFSPGKIARVPLLGNKTAIFEKEMMAYYDYRISADSLSHRPCYK
                     FSVEAKPQFLHSPRTVIKQLHTWFEKGSLKVVRRTYTLQAKTLLYQFKVDMKIDVKQT
                     AGKYYPTAIDYEGRWKVVSKKAEDALFRIRFSEFKPGES"
     gene            3293..4147
                     /locus_tag="PKM27_16990"
     CDS             3293..4147
                     /locus_tag="PKM27_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09019.1"
                     /translation="MIAICDSGGTKGDWVFIREETVHKLVAPGFNPYTHEIPQYLETL
                     QSLLHDISPEQVSQVHFYGAGCREENQQEKVRKGLAGLFSRAEIEVNQDLLAAARATC
                     GHSRGIACILGTGANSGLYDGARFIDNIPTLGYLAGDEGSGAHFGKLLIKAYFYREMP
                     AAVRAAFDEFFPGRHEKILSHLYSKNTPNTFLASFMKFAVEHTGDPFIRTLLLKNFQD
                     FLDTQVSKYEGYRSLPVHFVGSVAHYFTDVLKECLERNGVRCGMILPRPMERLIEFHR
                     KKSHEDHP"
     gene            4131..4451
                     /gene="cutA"
                     /locus_tag="PKM27_16995"
     CDS             4131..4451
                     /gene="cutA"
                     /locus_tag="PKM27_16995"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNR09020.1"
                     /translation="MKTILEVQVTFPDETKAREICNQVIVEKLAACANIFPVQSRYIW
                     KENFLEETEYAALLKTDPEALVALTDRIKTLHPYEVPAITCWHTLAIPEYADWVYECC
                     RKPD"
     gene            complement(4912..5814)
                     /locus_tag="PKM27_17000"
     CDS             complement(4912..5814)
                     /locus_tag="PKM27_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNR09021.1"
                     /translation="MKYRLLFYCFILVASMPSALRAQEDLLSMIRDSASTEKQPVKSS
                     FKTTRVVNFQSLENTHARNLDIKISHRFGFLNLGIEELFGLDAATIRLGAEYGITDRL
                     MVGFGRSSFEKTLDTYFKWKTIQQTSGKGGMPVTVSLFGSAALRTFRVNTDLEDKLLN
                     RMYYTGQVIVGSKISEEFSLQLSPTIVHRNLTATTSEKNDVFGIGAGGRFKVSKRISL
                     NAEYMYLLPDQVAPGITNSLSIGFDIETGGHVFQLHLTNSTAMIEKGFITETTGDWGD
                     GDIHFGFNISRVFDFSRKNKTKAE"
     gene            complement(5847..6422)
                     /locus_tag="PKM27_17005"
     CDS             complement(5847..6422)
                     /locus_tag="PKM27_17005"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNR09022.1"
                     /translation="MQLKPVLIIAFLVSVTGSLPAQTGAGGRYFTRGAAIQFFSDSPL
                     EKIEASTNSGNCVLDLATGKLEAAVLINSFNFKKALMQEHFNENYMESSKFPKAVFKG
                     SLDKPQELTLSKDGTYKTMLKGEMTIHGITRPMQTEVVFRVGDGKIFAETAFDVTVAD
                     YGIRIPALMRDNIAKIVRLTVKSELQELKKT"
     gene            complement(6433..6858)
                     /locus_tag="PKM27_17010"
     CDS             complement(6433..6858)
                     /locus_tag="PKM27_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09023.1"
                     /translation="MKSKLTLLILILALSGVYIGYRMWNKPHLDIASAGPAASLTASE
                     LLAEYQSDESVANAKYLNKVISINGVIEAVSKEADQVSIRLATEDVMATVICNLDPFS
                     SHAETEFVTGRNINLKGVCSGILTDVVLDRCVVINPSFK"
     gene            7293..9848
                     /locus_tag="PKM27_17015"
     CDS             7293..9848
                     /locus_tag="PKM27_17015"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNR09024.1"
                     /translation="MQQIYFKPLFRAFLTAFGLCLAACSPVPADPSPPLSPEEAKDVF
                     QLEPGFEIQLVAAEPLVQDPVVIQFDEDGRLWVVEMRSFMPDIDGKGELEPNGRICIL
                     EDLDKDGLMDSSRVFLDSLILPRAMALVKGGLLVAENKALWLVEDQDGDLQPDRKLLL
                     DSTYATNGLPEHSDNGLWRGMDNWYYNAKSRLRYRLENGVWIRDSTEFRGQWGICHDD
                     EGRLYYNYNWSQLHADLVPPNYLSRNKNHIPGSGIDHGLTLDRRVYPVRPNPAVNRGY
                     IPGTLDSLGRLLEFTAACSPLVLRSAVFPPAFYQNVFVCEPSGNLVKRNILEENGFLL
                     SAHDPHPGKEFLASSDERFRPVYLASGPDGALYVADMYRGLIQHGAYVTPYLREQTLK
                     RNLVLPIHMGRIWRIVPEKHKRVSQVKMSAKTTDELVADLSHPDGWSRDMAQRLLVER
                     NDPACIPALVKLAEDGTQVLARNHALWTLDGLAWKDETILIRFIKDEQTLIANTALRL
                     LEPLAQKNNKVREVIEALFRETGSAPPAKKALQMALSAYVLDPEIRIKQLARLLDLHL
                     QQAVMRDAVISSLQGLEYRFLIYLLGLESWKTRDPAREIFIDLLTAAVIRHGKDHELK
                     GLLSLLDPGKGSFEWKQQAILNAMSIQGAAPPINPVKLSTAPALLTRSGLDQQNKNQM
                     EGAMQLFDWPGKIKKPTGGSGALLNDRDRQWFVKGRSLYLNSCAGCHGNDGAGINRFA
                     PTLRGSSWVTGNEKRLALLVLHGIEGPIEVNGKIYDAPEILPVMPSHSTMDDGDIQAI
                     LTYIRNEWGNQAGPVSRNLVGMTRHTTQGRVMPWTAEELNRHIADTLVVPENK"
     gene            9906..10967
                     /locus_tag="PKM27_17020"
     CDS             9906..10967
                     /locus_tag="PKM27_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNR09025.1"
                     /translation="MERREFIKNTGLAMAALPWFEIPGSLKVSSMGIVVHSYGIRWNS
                     KTESRNYPGFKNALDLLEHCQEIGAGGLQVMVRGWEKDFTQKIRDQREKYGLYLEGSI
                     GLPKTQAEIPAFETDILNAREAGASVIRTVCLPGRRYETFKSEEEFKTFRQNAIQSLQ
                     WAEPVMRKAGIKLAVENHKDWLAPELAGIIRQISSEWIGVTLDFGNSIALLEDPMEVV
                     KTLAPYAVSTHVKDMGVQEYEEGFLLSEVPLGKGILNLNEIVRICRQHNPGIRFSLEM
                     ITRDPLQIPCLMDKYWSTLPEVKANALANMLHLVRKNKYDGPLPAVSHLGPEEKLKAE
                     EDHVLACLAFSKTGLGLNP"
     gene            10987..>11221
                     /locus_tag="PKM27_17025"
     CDS             10987..>11221
                     /locus_tag="PKM27_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR09026.1"
                     /translation="MDKIKLPGIRQFDLTGQAAIVTGGSKGLGLAMAAGLASAGANVL
                     IVSRTAQEAREAAEGLQAEYGIRAEWFAADVTRQ"
BASE COUNT         2910 a         2909 c         2866 g         2536 t
ORIGIN      
        1 gtatggcgta agaagcatcc caacgcttct gtttttcaaa aacggtaagc tggtggatcg
       61 ccacgtgggt gcaaccagta aaaaggtcct cacggacaag ctggaagccc tcatgtctta
      121 agctttcctt accggaatca cccagatatt ctgcctatct tgcaggccgg gatgagaaga
      181 ttggaagaac ccggcatcag ggaaataagt aaactggaac tgcttgcaaa ccaggtggtg
      241 gaaggcttta ttgtgggcct tcaccagagc ccttttcacg gattttccgt ggaattcgcc
      301 gaacaccgga tctacaacga aggagaaagc actaaaaaca tagactggaa ggtgtttgca
      361 aggacggacc gtctttacag caagcgtttt gaagaagaga ccaacctccg ttgccagatc
      421 gtggtcgacg ccagttcttc catgcaattt cctaaagccg cttcggaaaa ccctctggac
      481 agccagaata agtttgtatt ctccgcactg ggcgccgcct ccctcatgag cctgttgaag
      541 cggcagcggg atgcctttgg gctggacctt tttgatgaaa ccttaaaaat ccataccgaa
      601 gccaaatcca gcacggccca ttaccggctc ctgctctctc accttgaaaa agtcctgaat
      661 gaccccgggc agcacaaagg cacccggacg gcacaggtgc ttcacgagat cgccgaccgg
      721 atccataaac ggtcgctggt gatcattttt tcggatatga tggacgaccc ctcccatatc
      781 cgcgaagtgt tttcctccct gcagcatctc aaacacaata aacacgaagt cattcttttc
      841 cataccgtgg accataacaa ggaaatcgag ttccaatacg aaaaccggcc ccatcttttc
      901 atcgacctgg aaaccggtga aaaagtcagg ctgctgccca gggaagtgga aaaggaatac
      961 cgccggcaaa tggcggccct ctacgaccag atccggatga cctgcctgca gtacaaaatc
     1021 gacctggtcg aagccgacat cgccaaaggc tataaaccca tcctgcaggc atacctggcc
     1081 aaaagaagcc ggatggcggt gtgagcaccc ctttatcctt tttatctttg atcccaatcc
     1141 aatccaaaca ttgatgcata aactcaagct gatctccacg aaagcgccga aaggcatgga
     1201 caaacaagct accaagaccc tcctcaaaca catgaccaaa gagatcgggg attaccagca
     1261 cgccctgtac gcggaaaaaa aacaaagtct cctggtcatc ttccagggag tggatgcgag
     1321 cggcaaggat ggctcgacca aaaaagtttt tgaggaatgc ggcaccatcg gcatcgatgc
     1381 ctttgggttc aagaaaccca ccgaagagga gtttgcccat gacttcctct ggagggtgca
     1441 ccagcgggcc cctgcgaaag gatatattca gatctacaac cgttcgcatt acgaggacat
     1501 cattatccaa cgggtgcaca aatggataga tctgaaaagg gttaaacaac gcatggacgc
     1561 gatcaacgcc tttgagcaat tgctggaatt tgacaacaac accaaggtgc tcaaattcta
     1621 catgcatatt tcaaaagaaa aacaaaggat caaactgcag gaacggattg atgatcccgc
     1681 ccgcaactgg aagcataaca gcgtcgactg gaaagaggcc ctgcactggg acgaatacat
     1741 gaaagcctat gaatacgcca taaacaacag cgtcattcca tggcatgtcg tacctgtgga
     1801 ccagaaatgg taccgcaatt tttatattgc caaaatcgtg ctccagaccc ttaaaaaaat
     1861 gaacccgcag ttgcccttgc tgagcgcaga agacaaacgg gtcatggccg aaatccaggc
     1921 caaccttaaa taaccatgga tcaggtcagg gcggacaaat ggctttggag catccggttg
     1981 tttaagaccc ggacggtggc cgccgatgcc tgtaaggaag gccgggttta ccgcggtgaa
     2041 caagcattaa aagcctcgag cgaaatcaag gccggtgacc tgctccgggt tcacaaagac
     2101 ggcttccggc tgatgatccg tgtgaaagat ttgttggaaa aacgggtcag cgcccagctg
     2161 gcgcgcgaat actatgagaa cctaaccccg gaagaggaac tgaataaata caaatcctgg
     2221 tttataggaa aagccagcgc tgaggtgagg gaaaaaggcg ccggaaggcc caccaaaaag
     2281 gaaagacgcg aaatcgacgg ctataaagac accgcctttg accaggagat cgaaccctgg
     2341 cttgaggaat gaattcgaat cttccatatt ctgtccgttt cctttccctg ttttttatat
     2401 ggtgccggcc cgtttcagcc accgcccagg aggaccccgc agcgatttac cggcacatcc
     2461 atctggactc cgtggtgatc accgctgaac gcgggggttt cgatgttctc gattttattg
     2521 agctggtgct gagggacacc agtttttatg aggcattccg caatctcaga ttcgcttcgt
     2581 attcctttga aaacaaactt gtgatcctgg ataagtcccg gcaggaaaag gcgggatacc
     2641 gaagcctggc ccaccaggtc tacgactcat cctgctggca gatgaaggaa gaacaaaggt
     2701 cggtctccgg gcctttcctg gatcccaaag gggattatcg attttatacg gcaaaacttt
     2761 atgaccggct tttttttacc cacgggaaaa tatgtcatcc cgcaccggcg gacgtgcagg
     2821 ataccaggtc gaaaggaatg gaaaaacacg tggaagaact aaaaaccctc attttctcac
     2881 cggggaaaat agcccgcgta cccttgctgg gaaataaaac tgctatattc gaaaaggaaa
     2941 tgatggccta ttacgattac aggatcagtg cggacagcct ctctcaccgg ccctgctata
     3001 aattctccgt ggaagccaaa ccgcaattcc ttcattcccc ccggaccgtc atcaaacaat
     3061 tgcatacctg gtttgaaaaa gggagcctca aggtcgtgcg tcgaacctat accctgcagg
     3121 ccaaaacctt gttatatcaa tttaaggtgg acatgaaaat cgacgtcaag caaaccgccg
     3181 gcaaatatta ccccaccgcc atcgattacg agggccggtg gaaggtggtt tccaaaaagg
     3241 cggaggatgc cctgttccgg atccgctttt ctgaatttaa acccggagaa tcatgatagc
     3301 catttgcgac agcggcggca ccaaaggcga ctgggtattc atccgggagg aaacggtgca
     3361 taaactggtt gcaccgggtt tcaatcccta tacccatgaa attccccaat acctcgagac
     3421 cctgcaatcc ctgctgcatg atatatcccc ggagcaagtg agccaggttc atttttacgg
     3481 agcaggctgc cgcgaagaga accagcagga gaaggtccgg aaaggactgg ccggtttgtt
     3541 ttcaagagcg gagattgaag tcaaccagga cctgctggct gctgccaggg ctacctgcgg
     3601 ccattcccgg ggcattgcct gcatcctggg gaccggggcc aactccgggc tgtacgacgg
     3661 ggcacggttc atcgataaca ttcccaccct cggctacctg gcgggagatg aaggcagcgg
     3721 cgcccatttt ggcaaactgc tgatcaaagc ctatttctac cgggaaatgc cggctgcggt
     3781 ccgtgccgca tttgatgaat ttttccccgg caggcatgaa aaaatcctgt cccaccttta
     3841 ttcaaaaaat acccccaata ccttcctcgc ctcttttatg aaattcgcgg tggaacatac
     3901 cggggaccct tttatccgca ccctgttgct caaaaatttt caggatttcc tggataccca
     3961 ggtaagcaaa tacgaaggat accggtcact gccggtacat tttgtcgggt cggtggccca
     4021 ttattttaca gatgtcctca aagaatgcct ggagcgaaat ggggtgcgtt gcgggatgat
     4081 cctgcccagg ccgatggaaa ggctgattga atttcaccga aagaaaagcc atgaagacca
     4141 tccttgaagt acaggtcacc tttccggacg aaacgaaggc acgcgaaatc tgcaaccagg
     4201 tcattgtcga aaaactcgcg gcatgtgcca atatctttcc cgtgcaaagc aggtatatct
     4261 ggaaagaaaa cttcctggaa gaaacggaat acgccgcctt gctcaaaacg gatccggagg
     4321 cactggtagc cctgaccgat aggatcaaaa ccctgcatcc ctatgaagtg ccggccatca
     4381 cctgctggca taccttagcc attcctgaat acgcggactg ggtttatgaa tgttgccgta
     4441 agccggatta aagcagcaac tggcaaattc aatggaaaac caggagaaaa taaactataa
     4501 taccaaggag gaagtcttaa agggcttacc acccaccaac cacctcccgc tggagctatg
     4561 gcccccggct aaagccgagg gtatcccgaa tgtaattcgc gcggaggaca cggggagcac
     4621 ggagaatgtt ttttcaactg aatatgtagg aagtgatctc cgtgtcctcc agccctccgt
     4681 gcgccaaggt accatttctc caaacccggc cttcaggctg gggttgggag gattcaagga
     4741 ggatttgggc tttagcccaa atcggttgga tggcttgctg ttggtaaagc gtacacgctg
     4801 ggccaaagcc cagaggcttc tctgatgcca actcccaggc gaaaaaccat gggattgact
     4861 atacttctag ctgcccccta ctgttccgaa attctccatg ctggataccc cctactcggc
     4921 ctttgtttta ttttttctgg aaaaatcaaa tactcttgaa atattaaacc cgaagtgaat
     4981 gtccccgtca ccccaatctc cggtcgtttc cgtaatgaat cctttttcaa tcatggcggt
     5041 ggaattggtg agatgcaact ggaagacgtg tccccccgtc tcaatgtcga agccgatgga
     5101 cagggagttg gtgatgcccg gagccacctg gtccggcaac agatacatgt attcggcatt
     5161 cagagaaata cgtttggata ccttaaacct tccccctgcg ccaatcccaa acacatcatt
     5221 tttttccgag gtcgtcgccg tcaggttgcg atgcactatg gtgggtgaga gctgaaggct
     5281 gaactcctct gaaattttgc tgcccacaat gacctgcccg gtataataca tacggttcag
     5341 caatttgtcc tcgaggtcgg tatttaccct gaacgtacgc agcgccgcgc tgccaaacag
     5401 ggaaaccgtg accggcatcc ctccttttcc actggtttgc tggatggtct tccatttaaa
     5461 gtaggtatcg agcgtttttt caaagctgct tcgtccgaat cccaccatca gccggtcggt
     5521 aatcccgtat tccgcgccga ggcggatggt ggcggcgtcc aggccaaaaa gttcttcaat
     5581 accaagattg agaaatccaa aacggtggct gattttgata tccaggtttc tggcatgggt
     5641 attttcaagg gattggaaat tgaccacccg ggtagtttta aaacttgatt ttacgggttg
     5701 tttttccgta gatgcggaat ccctgatcat ggacaaaagg tcttcctggg cacgaagcgc
     5761 tgagggcata gaagccacaa ggatgaagca ataaaagagt aagcggtatt tcataacatt
     5821 tttagattat tgaatgaata ggaggtttag gtttttttta attcctgtaa ttccgatttg
     5881 accgttaagc gaacaatttt agcaatatta tcccgcatca gggccgggat acggatcccg
     5941 tagtcggcca ccgtgacgtc aaatgcggtt tctgcaaata tcttgccatc tcccacccgg
     6001 aagactacct ccgtctgcat gggacgggtg atcccatgga tggtcatctc ccctttaagc
     6061 atggttttgt acgtcccgtc ttttgaaagg gtgagctcct ggggtttatc caggcttcct
     6121 ttgaaaacag ccttggggaa tttggaggac tccatataat tttcattaaa atgctcctgc
     6181 atcaaggctt tcttaaaatt gaaggaattg atcaggacgg cggcttcaag tttgcccgta
     6241 gccaaatcca gaacgcaatt tccgctgttg gtagaggcct ctattttttc aagcggactg
     6301 tctgaaaaaa actggatggc agctcctctg gtaaagtagc ggccccctgc gccggtttga
     6361 gccggcaacg atccggtcac ggataccagg aaggcgatta taaggacggg ttttaactgc
     6421 atatccccgg ttttatttga aagaagggtt tattaccacg cagcggtcta agaccacatc
     6481 ggttaaaatc ccggaacata ccccttttag gttgatattc cggccggtga caaactccgt
     6541 ctccgcatga ctggaaaagg gatccaaatt gcagatcacc gtagccatga catcctccgt
     6601 ggcaaggcgg atgctcacct gatccgcttc cttgctcacc gcttcaatga ccccgtttat
     6661 ggaaatgacc ttgttcaaat acttggcgtt ggcgactgac tcgtccgact gatattctgc
     6721 gagtaattcg gaagccgtca gggatgctgc cgggccggcg gatgcgatat ccaggtgagg
     6781 cttgttccac atccggtatc cgatataaac ccccgacagg gcgaggatca ggatgagcaa
     6841 ggtcagtttc gatttcatgg gtggacatgt ttattcaact tagccctgat tttctccttt
     6901 tcattgagat caggctataa tattccggaa taacaataat gaacaaagta aatatggaat
     6961 agtaacgatt cagcagactg gaattgactg aaaaaagcgt taatttccta tgatccccca
     7021 tggcttcggg tcaaatctca atcttttcct ccgcactacc tataaaaacc ggctttctaa
     7081 gccctttttt aggaatggct ttcctgcttc ctttcctaaa aggcacggga gttcaggtaa
     7141 atgtgaaggg atggtttgct ttggttccgt ggaacttcgt gtcgttgtgg gatctttgca
     7201 gtcaaatctc aactgtgaat caagctgcag ggcaatacca attccttaac tgtgaaaagc
     7261 aataaccatt ttcattgtaa cttcatgccc ggatgcagca gatttatttt aagccccttt
     7321 tccgggcctt tttgacggcg tttgggctgt gtttggccgc ctgcagccca gtccctgcag
     7381 acccttcgcc accactctcc cccgaagaag caaaagacgt ttttcagctc gaaccgggat
     7441 ttgaaatcca actggttgcc gctgaaccgc tggtgcagga tccggtagtg atccagtttg
     7501 atgaagacgg ccgcctctgg gtggtggaaa tgcgcagttt tatgcccgat atcgacggta
     7561 agggagaact ggaacccaac ggaaggatct gcatccttga ggacctggat aaggatggat
     7621 tgatggacag cagcagggtt ttcctggaca gcctcatcct gcccagggcc atggcccttg
     7681 tgaaaggagg actgctcgtg gctgaaaaca aagccctttg gctggtggaa gaccaggatg
     7741 gcgacctcca gccggaccgt aaacttttat tggattcgac ttacgctacc aatggcttgc
     7801 cggagcattc ggataacggg ctctggagag ggatggacaa ctggtattac aatgccaaat
     7861 ccaggctccg ataccggctt gagaacggcg tatggatccg cgacagtaca gaattcaggg
     7921 gtcagtgggg aatttgccac gatgatgaag ggcggcttta ttacaattac aactggtctc
     7981 aactccatgc cgacctggtc ccgccaaatt acctttcaag gaataaaaac catatcccgg
     8041 gcagcggcat tgaccatggg cttaccctgg accgaagagt atatccggtc agacccaatc
     8101 cggcggttaa caggggatac attcccggca ccctggacag ccttggccga ttgcttgaat
     8161 tcaccgctgc ctgttcccca ctggtattga gatctgccgt tttccccccg gcattttatc
     8221 agaatgtttt cgtctgtgaa ccttcaggca acctggtcaa gcggaacatc ctggaagaaa
     8281 acggtttcct tttatcggcc catgatccac acccgggaaa ggaatttctc gcttcctccg
     8341 acgaacgatt tcgcccggtc tacctggctt cgggacctga cggcgccctg tatgtagcgg
     8401 atatgtacag gggactcata caacacgggg cctatgtgac cccctacctg cgcgaacaga
     8461 ccctgaaaag aaacctggtc ctgcctattc atatgggacg gatctggagg atcgtccccg
     8521 aaaagcataa acgggtaagt caagtaaaaa tgtcggctaa aactacggat gaattggtgg
     8581 ccgatctatc ccacccggat ggttggtccc gggacatggc ccagcggttg ctggtggaaa
     8641 gaaatgatcc tgcctgcatc cctgccttgg taaaacttgc cgaagacggg acacaggtcc
     8701 tggcaagaaa tcacgcactg tggaccctgg acgggctggc ttggaaggat gagaccatcc
     8761 tgatccggtt tataaaggat gagcaaaccc tgatcgcgaa taccgccctc cgactgctgg
     8821 aacctttggc gcaaaagaat aataaagtca gggaggtcat cgaagcatta ttcagggaga
     8881 caggttcagc accgcctgcc aaaaaggccc ttcaaatggc cttatccgcc tatgtactcg
     8941 atccggagat caggatcaaa cagctcgccc ggctgctcga tcttcatctc cagcaagccg
     9001 tgatgcggga tgcagtgatc agcagcctgc agggtttgga gtaccggttc ttaatttacc
     9061 ttctgggcct tgaatcctgg aaaacccgcg atccggcaag ggaaatattt attgacctgc
     9121 tcaccgcggc ggtcattcgc catgggaaag accatgaact taaaggcctc ctctccctgc
     9181 tggatcccgg gaagggctct ttcgaatgga aacaacaagc catcctcaat gccatgtcca
     9241 tccagggggc cgcgccaccg attaatcctg taaaactcag cacggcgcct gccctcctta
     9301 caaggtccgg gttggaccaa caaaataaaa atcaaatgga gggggccatg caattatttg
     9361 actggcccgg aaaaataaag aaacccaccg gaggttccgg ggccttattg aatgatcgtg
     9421 accggcaatg gtttgtcaag gggcgcagtc tgtatttgaa ttcctgtgcc ggttgtcacg
     9481 ggaacgatgg ggccgggatc aaccggtttg cgcccaccct gcgcggttcc tcctgggtga
     9541 ccggcaatga aaaacgactc gctcttttgg tcctccatgg catcgaaggc cccattgagg
     9601 tcaatggaaa aatttatgac gccccggaaa tcctgcccgt gatgccctcc cattccacca
     9661 tggacgacgg agacatccag gccatcctga cctatatccg taacgaatgg ggcaaccagg
     9721 ccgggccggt gagcaggaac ctggtaggca tgacgcggca tactacccag ggcagggtga
     9781 tgccctggac ggcggaggag ctgaacaggc atatcgcaga taccctcgtc gttcccgaaa
     9841 acaagtaatt ttagcaggat gaaacacaga agatcaggat tttaaaagcg gatatttaca
     9901 tttttatgga aagaagagaa ttcattaaaa atacgggact ggccatggcg gccctgccat
     9961 ggtttgaaat ccccggctcg ctcaaagtga gttccatggg catcgtggtc cactcttatg
    10021 gtatccgctg gaactcgaag accgagagca ggaattatcc cggttttaaa aacgctctgg
    10081 acctcctgga acactgtcag gaaattgggg ctggcgggct tcaggtcatg gtcaggggct
    10141 gggaaaaaga ttttacccaa aaaatcaggg atcagaggga aaaatacggc ctttacctcg
    10201 aaggttccat cggccttccc aaaacccagg cggaaattcc ggcctttgaa accgatattc
    10261 tcaatgcccg ggaagccggc gcttcggtca tccgcacggt atgccttccg ggccgccggt
    10321 acgaaacttt taaaagtgaa gaggaattta aaaccttccg ccaaaacgcc atccagtccc
    10381 tgcagtgggc ggagccggtc atgcgcaaag cggggataaa gctggcggtt gaaaatcata
    10441 aagactggct ggcccccgag ctggccggca tcatcaggca gatcagcagt gaatggatcg
    10501 gggtcaccct ggatttcggc aacagcattg ccctcctgga agatcccatg gaggtcgtca
    10561 aaaccctggc gccttatgct gtgagcaccc acgtgaagga tatgggcgtg caggaatatg
    10621 aagaagggtt tttattatcc gaagtacccc tcggcaaagg catcctgaac ctgaatgaaa
    10681 tcgtccggat ttgccggcaa cacaatccgg gcatccggtt cagcctggaa atgatcaccc
    10741 gtgatccgct ccagatcccc tgcctgatgg ataagtactg gtctaccttg ccggaggtga
    10801 aggcaaatgc cctggcaaat atgctccacc tggtcaggaa aaataaatat gacggcccat
    10861 taccggcggt ctcccatctt gggcctgagg aaaaacttaa agcggaggaa gaccatgtgc
    10921 tggcctgtct cgctttcagc aaaacgggac tggggctcaa tccctgattt aataaaataa
    10981 caggatatgg ataaaatcaa actccccggt atccggcaat tcgacctgac gggtcaggca
    11041 gccattgtca ccggaggttc caaaggtctg ggccttgcga tggccgccgg gcttgcttcg
    11101 gccggggcca atgtactgat tgtttccaga accgcccagg aagcccggga agcggctgaa
    11161 ggccttcagg cggaatatgg catccgggcc gagtggtttg cagctgacgt gacccggcag
    11221 g
//
LOCUS       DAOQXT010000130        11192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6258_length_11192_cov_7.699470, whole genome shotgun sequence.
ACCESSION   DAOQXT010000130 DAOQXT010000000
VERSION     DAOQXT010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6258_length_11192_cov_7.699470"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..947)
                     /locus_tag="PKM27_17030"
     CDS             complement(78..947)
                     /locus_tag="PKM27_17030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR09027.1"
                     /translation="MQWNIPIYLLAVLSLASCKKSAPDFTEGQVSLEPGMKMSYYRCG
                     QGDTTLLFIHGWGINKSYWKAQLDAFCPQYQVVAIDLPGFGQSGKDRTDWTFDQYAGD
                     VKTLMDQLDLKKVILVGHSMSGDLILRVATIYPEPIIGLIGIDNLHQPGGPMDSAQVA
                     EAEGFFAMMLKDFDSTVVKTMKTNLFQPSTPDSIVQRVMNDVFAADSVIAVKVLMGMT
                     KMAQAEQAMMQQLQVPLCLVNSDVFPTDLESLNKYCAKGVKLYTVKGTGHYPMLEKPR
                     EFNQALQRAILRL"
     gene            1015..1491
                     /locus_tag="PKM27_17035"
     CDS             1015..1491
                     /locus_tag="PKM27_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNR09028.1"
                     /translation="MNLHFLFALSLIAACHTTGPKVPSDDHDLSSSQDSARAASWGAD
                     PYGMKQYVLVLLKAGPNRSQDSLTRANLQKAHMENITRLANEGKLILAGPMMDDGEIR
                     GIYVFDVRSLEEARALTETDPAIQAGSLVLEMHPWYCTAALMDLPRLHKLIAKKDI"
     gene            complement(1495..1809)
                     /locus_tag="PKM27_17040"
     CDS             complement(1495..1809)
                     /locus_tag="PKM27_17040"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HNR09029.1"
                     /translation="MNIEKNLNKDLILREKLALQRTALSNQTTFLAYIRTSLYFVVAA
                     LSIQNLLRIENGWYLELGFFVASGIILGLGLISYFHHRKRIEESKRHIGHYQDEFLDK
                     AQ"
     gene            complement(1847..2719)
                     /locus_tag="PKM27_17045"
     CDS             complement(1847..2719)
                     /locus_tag="PKM27_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09030.1"
                     /translation="MKKINAVTLLTFSIAVMAASATVSSIFFSEGPGPYEFLSIRGQT
                     VMIYGKGIYRHMSAEVAPQGIAQDYVTLFIALPLLLVSLFKSWRGSLAYRVLHTGCLA
                     YFLVTYLFYLMMAMYNGLFLVYAALLGSSFFAFIISLNAMDRPLTNRFESYPYRKFTG
                     GFLMFNALAIALLWMGIVVPPLMDGTLIPMETRHYTTLVVQGLDLAILLPSAFVLGWL
                     FWKKQKTGDLLTPVYYVFLSILLTALMAKIIAMGLLGYSIFPVVFIIPVFLTLTLVNT
                     GLILKQLVTKSRGS"
     gene            2828..3505
                     /locus_tag="PKM27_17050"
     CDS             2828..3505
                     /locus_tag="PKM27_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09031.1"
                     /translation="MSERVRIAIWISLLGFILSGPVGLVLVTWFGPQPEWTSAVIFAE
                     HYREIQALPYYFGFFLIGGMVMLVLAHFLDYRQKKDPIAWKPGLSLGLVLVFATLITF
                     NYICQTTFVRQMATHYQPLYEPLLAAFSMSNPFSLAWAIEMWGYGILGISLWLLSDQY
                     REKNRWIPPLLLLNMILSLASLAWTIVDARWVLTGVGLGLYLAWNVLMILILAMILKQ
                     SGKFSST"
     gene            3502..4623
                     /locus_tag="PKM27_17055"
     CDS             3502..4623
                     /locus_tag="PKM27_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09032.1"
                     /translation="MRILLFILILVHGLIHLMGFTKAFQLARLDQLSMPVSRFSGLIW
                     GLSAFLLVAAAVLYLFWLNAWWIPGTVGVLLSTGLILNYWKDSKWGMIPNLVLMTVLF
                     VAVAGYFFRHRFVKDARHVLSTLDSRPGNPLTLEDLAGLPDAVKNYIVYSGTVGKPRI
                     QSFRAVLDAQIRADRNAAWIPLEVEQYNRIGPLSRLFFMNGKMKGLPVSGYHRFKDGH
                     ATMDIRLFSLLKVQRQEGKEMDIAETVTFFNDLCILAPGALISKKIEWEEIAPLQVKA
                     KFTEGGTRIEALLFFNEQNQLISFESEDRYYISKETGPGKVRWVTPVSDYKDYHGYRL
                     ASRGEAIWYLPDGPLAYLRVELRDLRYNSPYHPVSREDL"
     gene            complement(4618..5025)
                     /locus_tag="PKM27_17060"
     CDS             complement(4618..5025)
                     /locus_tag="PKM27_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09033.1"
                     /translation="MAEAASLYSIPSRLRRLENMHIVFWLFKDISWCLDFRPLGVLMI
                     FPTLLVAIYITHKNRSIKSELAHNLAVIFWITANSTWMIVEFMGIDEQEIWKGVDGRH
                     LSVIPFAIGMLILAYYYLFQRKSELRGDREVTL"
     gene            5217..6116
                     /locus_tag="PKM27_17065"
     CDS             5217..6116
                     /locus_tag="PKM27_17065"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNR09034.1"
                     /translation="MKTLSARKKQYGAQLFTMPGMVSRDFRGTLELLSKIGYREIEFF
                     GPYPFSAPETIESWKPLRAQLGIQDNAFYGFSVKQTRDLLKEFGLSTPSIHLDLATLR
                     KNLKPMLDAAAELGVKYVTNPALWEEERRTLDDYKKRIDEFNEFGRQMKPYGIWFVYH
                     NHGYEEAKKEGKVPFHLLLENTDPGLVKFELDVFWMNASGASPMDYLKAWPGRFKLLH
                     LKDASEPIRFSGDGGSPDQWMSLFPKMTDPGDGVFNIPAIVRQALKSGAEHFFLERDL
                     APDPDKTLRHSFENLQAIEWPED"
     gene            6159..8507
                     /locus_tag="PKM27_17070"
     CDS             6159..8507
                     /locus_tag="PKM27_17070"
                     /inference="COORDINATES: protein motif:HMM:NF027641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNR09035.1"
                     /translation="MKPSLIIFLLGFLSFSVHSQKAIRFGQPGSYLEVEDHASLRLPV
                     MTLECWLKINQTGNPAFADGEQTLFDKRGNGGGFNLRLAGTRFPLPVFGFFDPEVQGH
                     LPALINQKRWIHLAYVLSSDSVFIYLNGNRQTAVKKQGNYQENSGSALRIGEFLGYPG
                     ASLGLQGDMDEIRIWNHVRSAAQIQADMHKTVQTSSGGLRLYLNFETISNQRILDQSG
                     NGNHAKVQGNAMLIDSKAPIGYVPLPAPAGLRMIGATANILLEWEPVQGASAYRIYRS
                     ESPEFSTEDSPVFAAVGNTGTYTDVSAQTGKTYFYTVTAVDANGREGLAGAVAAARRI
                     LPEDYTTGVYYYPWYIPEENHHPWPEQYIRYFLKPAQAPLLGHYSVKNPETVKKHFHW
                     MTASGIDFIVSSWWGPSSFEDLVLKNTLLQQLESSSLKFCVYYESAILGFGPNGIDIS
                     GNKENDLLDHFNYLEQTYFKSPAYLRIDNKPVVFLYLAGIYSGNYLQAFQKVRTAMAS
                     KGVSLFLIGDVDGWTQTDSVRLSFMDGISPYVTLGKTGYPVETNFPADVSMAHYHRQQ
                     ELINYNKLYIPNITPGFNNICVPCDKGFNWPRQISSTAPAESTLEYAIKAARPFIDPA
                     RKMILITSWNEWHEDSQIEPTVTAPPTSLDNSPGGMGYTRGYSYAGYGFRPLSLVAEL
                     LGNQKMVVPVNTIPAIAPGFNIYPSPARDKIVVEFYQALKKKTKIRITDLQGRTLSAA
                     ADPLNPVAEFKYVYSIQNLPPGLYTFYAEGYGSRKFIKVNQE"
     gene            complement(8547..9263)
                     /locus_tag="PKM27_17075"
     CDS             complement(8547..9263)
                     /locus_tag="PKM27_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR09036.1"
                     /translation="MTMNRNKILLVEDDTSLGPLLLDYLESEDYQVSWKRDGMTALDQ
                     LRKQAYDLCLLDIAMPGLDGFNLARQIKNQFKQLPFLFLTARSLKEDKLKAYELGAED
                     FITKPFDPDELACKLKVILRRKQQEHIPETMQVGQFTFHTSMQELTFGAKAIKLTEKE
                     NQILLMLSCNQNKIVRREDAVTRVYGKYDYFLGRSFDVFISRLRKILKDDPGIHIDNV
                     FRVGFILRVEERGLRKEERS"
     gene            complement(9260..10462)
                     /locus_tag="PKM27_17080"
     CDS             complement(9260..10462)
                     /locus_tag="PKM27_17080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR09037.1"
                     /translation="MRKVYTLIAASALALILLIFFQMRWIGTSKALIEEQFTNKVNLA
                     LCSAIEQLSESEDCRQTCLSMEGCSAKIQALAADSLFSSVLKSKLEFYQVDLPFEVNI
                     AVQDTAAGTAPPYSCSLMPLLAKDDHWLQLIFQGKKDYFNQRNGMMVTASMLILFGIG
                     ALFIYAAYWLVRQQKLSEANLQFFNHMTHEFRTPLTNIQLASRMMKKKSTSGSDPTYL
                     NIIDSQCDHLRHQVDNVLHMATLEKENFRLEMDEVDLRELAREVVDGMSMQIQDKKAS
                     VEITTSEQPCHIRGDRFHLRNVFHNLIENALKYSPALPRIEIGFFPFAQGWCARVRDH
                     GPGIRPEENEKIFQPFYRGGATESPIRGFGMGLAYVKKILDLHQGQIRVEAAPSQGTC
                     FEMYFPKA"
     gene            10680..11051
                     /locus_tag="PKM27_17085"
     CDS             10680..11051
                     /locus_tag="PKM27_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09038.1"
                     /translation="MKKLILFLPVWLAFVALPYQLDAQSCCQGAETSVKKTCSVSASQ
                     TLKPETLQAPASPSEASQEKSFSLVSAKPVLHLYSSLFASGIAALLSLVESCDPACCD
                     PADCDPSQCDPVDCLPLCEKK"
BASE COUNT         2780 a         2754 c         2790 g         2868 t
ORIGIN      
        1 tgcagctgcc ctctttggcc cgctaatgaa tatcaccggt tccctgcttg cgagttactg
       61 gagcaagaaa taacggatta tagccggagg atggcccgtt gaagggcctg gttaaattcc
      121 cggggttttt ctaacatggg ataatggccg gtgcctttca ccgtatacag ttttacccct
      181 tttgcacaat atttattcag gctttcgagg tctgtaggga agacatctga attcaccagg
      241 cagaggggca cctgcagttg ttgcatcatg gcctgttcgg cctgggccat ttttgtcatg
      301 cccatcagca ctttcaccgc aatgaccgaa tcggcggcaa atacatcatt cataaccctc
      361 tgtacaatgg aatccggtgt ggaaggctgg aacaaattgg ttttcatggt tttgaccacg
      421 gtggagtcga aatccttcag catcattgca aaaaagccct ccgcctccgc gacctgggct
      481 gagtccatgg ggccgccggg ctgatgaaga ttgtcaatgc ctataaggcc aatgatgggt
      541 tcgggataga tagtagccac ccggagaatc aggtcccccg acatggagtg ccctaccagg
      601 atcacttttt tgaggtccag ttgatccatc agtgttttta catccccggc gtattggtca
      661 aaggtccagt cggtacggtc ctttccggac tgcccaaagc cgggcagatc gatcgccacc
      721 acctggtatt gaggacaaaa agcatccagc tgggctttcc agtaagattt gttgatcccc
      781 catccgtgta taaacaggag ggtggtatct ccctgtccgc aacggtagta ggacatcttc
      841 atcccgggtt cgagagagac ctgcccttcc gtgaaatccg gagctgactt tttgcaggac
      901 gccaaagaaa gaacggccag gagataaatg ggaatattcc attgcataaa cgaaaacttt
      961 taagaatacc ggatatcaat aagccctccg gatatctttg tataaaaata cgatatgaac
     1021 cttcattttt tattcgcctt atccctgatt gcggcatgcc atactacggg gcccaaagtg
     1081 ccttccgatg atcatgacct ttcttcttcg caggacagcg cgagggccgc gtcctgggga
     1141 gcagaccctt atggcatgaa gcagtatgtg ctggtcttgc tcaaggcagg accgaaccgg
     1201 tcgcaggatt cccttacccg ggcgaacctt caaaaagccc atatggaaaa catcacccgt
     1261 cttgcaaatg aaggaaaact gatcctggcc ggtccgatga tggatgacgg ggagatccgc
     1321 ggaatttatg tctttgatgt gcgcagcctc gaggaagcca gggctctcac cgaaaccgat
     1381 ccggccattc aggcaggttc cctggtgctc gaaatgcacc cctggtattg tacggccgca
     1441 ctcatggatc tgccccgttt acataaactg atcgctaaaa aggatatatg atgctcattg
     1501 ggctttatcc aggaattcgt cctgataatg gccaatatgt cttttgcttt cttcaatcct
     1561 tttgcgatga tggaaataac tgatcagacc aagccccaat atgattccgg aggcgacaaa
     1621 aaatcccagt tccagatacc agccgttttc aattcgaagc aggttctgaa tactgagggc
     1681 tgcgactaca aaatagaggg aggtgcggat ataggctaaa aatgtagttt ggttggaaag
     1741 ggcggtcctt tgaagggcca atttctcccg gaggatcagg tctttgttga ggtttttttc
     1801 aatattcatg ggtttccggt ttctgtgttt tattgttcaa ttgagtttag ctcccccggg
     1861 acttcgtgac aagttgcttc aaaattaatc ctgtattgac cagggtgagg gtcagaaata
     1921 ccgggataat aaaaacaacg ggaaaaatgg aatagcccag gagacccatg gcaatgattt
     1981 tggccatcag ggcggtcagc aggatcgata aaaacacata atataccggg gtcagcaggt
     2041 cgccggtctt ttgttttttc caaaaaagcc aacccaatac aaaggccgag ggcaataaaa
     2101 tggccaggtc aagcccctgc acgacaaggg tggtatagtg ccgggtttcc atgggaataa
     2161 gggtgccgtc catcagcgga ggaacgacga ttcccatcca aagcagggct atggccaggg
     2221 cattaaacat cagaaaccca ccggtaaact tcctgtaagg ataggactca aaccggtttg
     2281 tcaaggggcg gtccatggcg tttaatgaaa tgataaaagc aaaaaaggaa gaacccagga
     2341 gggccgcata cacaagaaac aaaccgttgt acatggccat catcaggtaa aaaagatagg
     2401 tgaccaggaa ataagccagg caaccggtgt gcaagaccct gtatgccaaa gaacctctcc
     2461 aacttttaaa tagagaaacc aggagaaggg gcagggcgat aaaaagggtt acataatcct
     2521 gggctattcc ctggggagcg acttcggcgg acatgtggcg gtatatcccc ttgccataga
     2581 tcatcaccgt ctggcctcgg atggaaagga attcataggg tcccggcccc tctgaaaaaa
     2641 atatggagga tacggtagcc gatgccgcca tgacggcaat gctaaaagtg agaagggtga
     2701 ccgcatttat ctttttcacc gggattttaa aattatcagc caggactttg tccgcttctg
     2761 accaagggca tccttcggcc tgaccttact caccgggccc ttattccttt tttaacaaat
     2821 ttgagagatg tctgaacggg ttcggatcgc gatctggatt tcattattgg gatttatcct
     2881 ttccggaccg gttggattgg ttttagtaac ttggttcggg ccacagcccg aatggacctc
     2941 ggcggttatt tttgcagagc attaccggga aatacaggcc ctgccctatt atttcggttt
     3001 cttcctgatc gggggaatgg taatgttggt gctggcccat tttttggatt accggcagaa
     3061 gaaggacccg atcgcctgga aaccgggcct atccctgggg cttgtcctgg tttttgccac
     3121 cctgatcact tttaattata tctgccagac caccttcgtc cgccagatgg caactcatta
     3181 tcaacccctt tacgaaccgc tcctggcggc cttttccatg tccaatccct tttccctggc
     3241 ctgggccatt gaaatgtggg gctatggcat tctcggtatt tccctttggc tgctctcaga
     3301 ccagtaccgg gaaaaaaaca ggtggatccc gcctttgctc ctgctcaata tgatcctgag
     3361 cctggccagc ctggcctgga ctatcgtgga tgcccgttgg gtgctgacgg gagtgggcct
     3421 cggcctgtat ttagcctgga atgtgcttat gatcttgatt ctggcaatga tattaaagca
     3481 gtccggtaaa ttttcctcca catgagaatc ctgctattta ttcttattct ggttcatgga
     3541 ttgatccacc tgatgggctt tacaaaagcc tttcaactgg caaggctgga ccagctttcg
     3601 atgcctgttt cccggttctc cggattgatc tggggcttaa gcgccttcct gctggtcgcg
     3661 gcagctgttt tatacctgtt ctggttgaat gcctggtgga tccccggtac cgtaggggtt
     3721 ttgttatcca caggccttat cctgaattac tggaaggaca gcaagtgggg catgatccca
     3781 aacctggtcc tgatgaccgt gttgtttgta gctgttgcgg gatatttttt cagacaccgt
     3841 tttgtgaaag acgcgcggca tgtcctttca accctggact cccgcccggg aaaccctctg
     3901 acactggaag acctggccgg gctccctgat gcagtgaaaa actatattgt gtattccggc
     3961 acggtgggaa aaccaaggat tcaaagtttc cgggctgtgc tggacgcaca aatcagggcg
     4021 gaccggaatg cagcctggat tccgcttgaa gtcgaacaat acaaccgtat cgggccgctc
     4081 agccgcctgt tctttatgaa cggtaaaatg aaaggtttgc cggtttccgg ctaccaccgg
     4141 tttaaagacg ggcatgccac catggacatc cgcttgtttt ccctgttgaa ggtccagcgc
     4201 caggaaggga aggaaatgga tattgccgag accgtaacct ttttcaatga tctttgtatc
     4261 ctggcccccg gagcccttat ttcaaaaaaa atagaatggg aggaaatcgc ccccctgcag
     4321 gttaaggcca aattcaccga aggcggaacc aggattgaag cccttttatt ttttaatgaa
     4381 caaaatcagc tgatttcatt tgaatccgaa gaccggtatt atatttcaaa ggaaacaggg
     4441 ccgggaaagg tccggtgggt gaccccggta tcggattata aggattacca tggttaccgg
     4501 ctggcctcca ggggagaagc gatatggtat ttgccggacg gacctttggc atacctccgc
     4561 gttgaattaa gggacctgag gtataattcc ccgtatcatc cggtctcccg ggaagaccta
     4621 taaggtgact tcccggtcgc ctcttaattc gcttttccgt tgaaagaggt aataataagc
     4681 caggatcagc ataccgatgg caaagggaat cacggataaa tgcctcccgt ccactccttt
     4741 ccagatctcc tgctcatcga tacccatgaa ttcaacgatc atccaggtgc tgttggccgt
     4801 gatccagaag ataaccgcca ggttgtgggc cagttcggat ttaatgctcc ggtttttatg
     4861 ggtgatgtag atggcgacga gcagggtggg aaatatcatc aatacgccca agggcctgaa
     4921 atccaggcac cagctgatat ccttaaataa ccagaatact atatgcatgt tttccaaccg
     4981 cctcaacctg gaggggatgg aataaagaga ggcggcttca gccatgttta ttgaaaattt
     5041 gggcgtgaat ataatacaga gaggtggatg gtgggtggtc ttggagattt ggcagatccg
     5101 gcctaactta tcgtcttaag tcaaaaccaa atatgaaacc cggtcctttt acagcctctc
     5161 ggcggaattt tcttaaaaca tccggatggg ctgcgatcag tctcggttcc ctgccaatga
     5221 aaactttatc tgccagaaaa aaacaatacg gcgcccagtt attcaccatg cccggaatgg
     5281 tcagcaggga ttttcgggga accctggagc tgctcagtaa aatcggatac cgggaaattg
     5341 agtttttcgg gccctacccg ttcagcgctc cggaaaccat tgagtcctgg aagcccctgc
     5401 gtgcccaatt gggtatccag gacaatgcct tctatggatt ttctgtgaaa caaacccgcg
     5461 acttgctgaa agaatttggc ctcagcaccc cctccattca cctggacctg gctactttgc
     5521 ggaagaacct aaaacccatg ttggatgcag cggccgaatt gggtgtaaaa tacgtgacca
     5581 accccgctct ctgggaagag gagcggcgga ccctcgatga ttataaaaaa aggattgatg
     5641 aattcaatga attcggaaga caaatgaaac cctatggaat atggtttgtc taccacaacc
     5701 atgggtatga agaagcgaaa aaagaaggaa aggtcccctt tcacctgttg ttggagaata
     5761 cagatcccgg cttggtaaag tttgagctcg atgtattctg gatgaatgcc agcggggcca
     5821 gtcctatgga ctatctcaaa gcctggccgg gccggtttaa attattacat ctcaaagacg
     5881 cctctgaacc catccgtttt tcaggcgatg gaggaagccc cgaccagtgg atgagcctct
     5941 tccctaaaat gaccgatccg ggggatgggg tattcaatat tccggccatt gtccgtcaag
     6001 cccttaaatc cggggcggaa cattttttcc tggaacgcga tcttgcaccc gacccggata
     6061 aaacactccg ccattcattt gaaaatttac aagctattga atggcctgag gattaattag
     6121 attttcacca gccccgatcc atgactgcaa taacctactt gaaaccttct ttgatcattt
     6181 ttttgctggg tttcctgtct ttttcagtcc attctcagaa agccatccgt ttcggtcagc
     6241 ccgggtccta cctggaggtg gaggatcatg ccagcctgcg gctgccggtg atgacgcttg
     6301 aatgctggct gaagatcaac caaaccggca accccgcttt tgccgacggc gaacaaactt
     6361 tgtttgataa aagaggaaac ggaggtggtt ttaatctccg gcttgcaggg acccggtttc
     6421 ccctgcctgt atttgggttt tttgatccgg aagtacaggg gcacctcccg gctttgatca
     6481 atcaaaagag atggatccac ctggcttatg tgctttcttc agattcggtc tttatttatc
     6541 tgaacggaaa ccggcaaacg gctgtaaaaa aacaaggcaa ttaccaggaa aattccgggt
     6601 ccgccttacg tatcggggag tttctcggct atccgggagc ctctctgggc ttacagggag
     6661 atatggatga aatccggatt tggaatcatg tccgcagtgc tgcccagatc caggccgata
     6721 tgcataaaac cgtgcaaacc tcttccggcg ggctcaggtt gtatttgaat ttcgagacga
     6781 tcagcaatca aaggatcctg gaccagagcg gcaatgggaa tcatgcaaaa gtccagggca
     6841 acgccatgct gattgattcc aaagcgccca tcggttatgt cccgctgcct gcgccggcag
     6901 gattgagaat gattggagca acggcaaata tcctgctgga atgggaaccc gtccagggcg
     6961 cttccgctta ccggatctac cggagtgaat cccctgaatt ttccactgaa gacagtccgg
     7021 tgtttgctgc cgtggggaac accgggactt atactgacgt atccgcccaa accggtaaaa
     7081 cttattttta tacggtcacg gccgtcgatg ctaacggcag ggagggcctg gccggggccg
     7141 ttgctgcggc aaggaggatt ttgcctgaag actataccac gggggtctat tattatccct
     7201 ggtatattcc tgaggaaaat catcacccct ggccggaaca atacatccgt tattttctta
     7261 aacccgccca agccccgctt ctgggacatt attcggttaa aaacccggaa acggtgaaaa
     7321 aacatttcca ctggatgacc gcttccggga tcgattttat cgtaagcagc tggtgggggc
     7381 cttcttcctt cgaagacctg gtattgaaaa acaccctgct tcagcaactg gaatcgtcgt
     7441 cattaaaatt ctgtgtttat tatgaatccg ccatcctggg atttggcccc aatggcattg
     7501 atatcagcgg aaacaaggaa aacgacctgc tcgaccattt taactacctc gaacaaactt
     7561 atttcaaatc cccggcctac ctgcgcattg ataataagcc cgtggtcttc ctttaccttg
     7621 ccggcatata ctccggcaat tatctccagg cctttcaaaa ggtacgtacg gccatggctt
     7681 ccaaaggggt ctcgcttttc ctgatcggcg atgtggacgg ctggacccaa acggattcgg
     7741 tccggctcag tttcatggat ggcatctccc cctatgtcac cctgggcaaa acgggatacc
     7801 cggtggaaac caattttccc gccgatgtat ccatggccca ctaccacaga cagcaggagt
     7861 tgatcaatta caataaactc tacattccca atatcactcc cggattcaat aatatctgcg
     7921 taccctgtga taaaggattt aactggccaa ggcaaatttc ttcaacggcg cccgccgaat
     7981 ccaccctgga atacgcgatc aaagcggccc ggccttttat tgacccggcc cgaaaaatga
     8041 tcctcatcac cagttggaat gaatggcatg aagacagcca gatcgaaccc accgttactg
     8101 ctccccccac ctccctggac aacagcccgg gcggcatggg ttatacccgt ggttattcct
     8161 atgccggcta tggattcagg cccctgagcc tggtagccga attgttgggt aatcagaaaa
     8221 tggtggtgcc cgtaaatacc atccccgcaa tagctccggg atttaatatt tatccctctc
     8281 cggcccggga taaaatcgtg gttgaattct atcaggcttt aaaaaagaaa acaaagatcc
     8341 ggattaccga cttgcaggga agaacccttt ctgcggcagc ggatcccttg aatcctgttg
     8401 cagaattcaa atatgtttat tcgatacaaa atttaccccc cggtttgtat accttttatg
     8461 cggagggtta tggcagccgg aagtttataa aagtgaacca ggaataaact tcgatcagga
     8521 tcgctgacca ctgttcgcgg attcctctaa cttctttcct ctttcctcaa ccctctttcc
     8581 tcaaccctta aaataaaccc taccctgaac acattgtcaa tgtgaatgcc cggatcgtcc
     8641 ttcaggattt ttctcaatct cgaaataaat acatcaaagc tcctgccgag aaaataatcg
     8701 tatttgccat aaacccgggt tacggcatct tcccttcgca caattttatt ttggttgcag
     8761 gacaacatca aaaggatctg gttttctttt tccgttagtt tgatggcttt tgccccaaaa
     8821 gtcagttcct gcatggaagt atgaaaggta aattggccga cctgcatggt ttcaggtata
     8881 tgttcctgtt gttttctccg gagaatgact ttcagcttac aggccagttc atcagggtca
     8941 aaaggtttgg tgataaaatc ttccgctccc agttcatagg ctttcagttt atcttccttc
     9001 aggctgcggg ccgtaagaaa aagaaagggc agttgtttaa actgattttt gatctggcgc
     9061 gccaggttaa acccgtccag gccgggcatg gcaatatcga gcaggcagag atcataagcc
     9121 tgtttacgga gttggtccag ggcggtcatt ccatctcttt tccagctgac ctgataatcc
     9181 tccgattcca ggtaatccag gagcaaaggg ccgagcgagg tatcgtcttc caccaataaa
     9241 atcttattcc tgttcatggt catgctttcg gaaaatacat ttcaaaacag gttccctggc
     9301 ttggtgcggc ttctacccgg atctggccct ggtggaggtc cagtattttt tttacgtaag
     9361 ccaggcccat cccaaatccg cggatagggc tttccgtggc ccctccccgg taaaagggct
     9421 gaaatatctt ttcgttttct tcgggccgga tccccgggcc gtgatccctc acccgggcgc
     9481 accatccctg ggcaaaaggg aaaaaaccaa tttcgatgcg gggcagggca ggactgtatt
     9541 tcaaagcgtt ttcaatcagg ttgtggaaga cgtttcgcag atgaaaccgg tcgccccgga
     9601 tgtggcaagg ttgttcggat gtggtaattt ccacggaggc ttttttgtcc tggatctgca
     9661 tactcatgcc atccaccact tcccgtgcaa gttcgcggag gtcgacttca tccatttcca
     9721 accggaagtt ttctttctcc agggttgcca tatggaggac attgtccacc tggtggcgga
     9781 gatggtcaca ttgactgtct atgatgttga gataggtggg gtcggaaccg gatgttgatt
     9841 tttttttcat catccgggaa gcaagctgga tattggtaag aggggtccgg aattcatggg
     9901 tcatatggtt gaaaaactgc agattggcct cgctaagctt ttgctggcgg accagccagt
     9961 aagccgcata gataaaaaga gcaccaatgc caaacaggat cagcatggaa gccgtgacca
    10021 tcatgccgtt cctttgattg aaatagtcct ttttgccctg gaaaatcagt tgcagccaat
    10081 gatcgtcctt ggccagcaag ggcatgaggg agcaggaata gggtggagcc gtacccgctg
    10141 cggtatcctg aacggcaata ttgacctcga aaggaaggtc cacctggtaa aattcaagtt
    10201 ttgatttcaa cacggatgaa aacagggaat cggcagccag ggcctgaatt ttagcagaac
    10261 aaccttccat ggaaagacaa gtttgacggc agtcttcgct ttccgaaagt tgttcaatcg
    10321 ccgaacacag ggccaggttc accttattgg taaattgttc ttcgatcagg gccttggaag
    10381 tgccgatcca cctcatttga aaaaagatga gcaagatcag ggccagggcg gaggcggcaa
    10441 tgagcgtgta gactttccgc ataataggat ctgtgatgtg gtgtaaaggt cataagaatt
    10501 acctgatcta agaccgtgtt taacagccgt ttaacaagtg gctaacaggt cctcacaagt
    10561 ttatccggcc tgcgggctga cttttgaacc cgtgatcagt atgtatgaat cggggtgatt
    10621 tatggtcctg attgcccgta taccggcgga cccggtaatt ttttaaatcc tttttattta
    10681 tgaaaaaatt gattttgttt ttacccgttt ggctggcttt tgttgcgctt ccttatcaat
    10741 tggacgccca atcctgttgc cagggcgccg aaacttcggt caaaaaaact tgttcggtca
    10801 gcgcttccca aacgttgaag ccggaaaccc ttcaggctcc ggcaagtcca agcgaggcgt
    10861 cccaggaaaa atcgttttcc ctggtcagcg caaaacctgt ccttcacctc tattcttcgc
    10921 ttttcgcttc aggcatagcc gccctgctca gtttagttga gtcctgcgat ccggcctgct
    10981 gcgacccggc ggattgcgac ccgtctcaat gcgatccggt ggactgtttg cctctgtgtg
    11041 agaaaaagta aagagaagtc ggttagttag caagttggct agttagcaag taaattaagt
    11101 ggacaagggg tcttgtggaa cgctccctat tctatcgaac ttgctaactt gctaacggtt
    11161 gatggcaata taaaaatgag tcactgaaaa gt
//
LOCUS       DAOQXT010000131        11013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6382_length_11013_cov_6.473992, whole genome shotgun sequence.
ACCESSION   DAOQXT010000131 DAOQXT010000000
VERSION     DAOQXT010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6382_length_11013_cov_6.473992"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..807
                     /gene="fabG"
                     /locus_tag="PKM27_17090"
     CDS             67..807
                     /gene="fabG"
                     /locus_tag="PKM27_17090"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNR09039.1"
                     /translation="MQLLENKVALITGGSRGIGEALVRKFASLGASVAFTYSSSSEKA
                     TALAASLAPAKVMAYQSDAASFTQAEQLVKKVLADFGKIDVLINNAGITRDTLMLRMT
                     EEQWDEVIRVNLKSAFNLTKQVLKPMLGARAGSIINMTSVVGVFGNAGQANYAASKAG
                     MIGFTKSIAKELGSRNIRCNAIAPGFIETDMTHALDDKTRELYFSSLAIKRFGTAEEV
                     ADLAAFLAGDTSKYITGQVISICGGLNM"
     gene            810..2042
                     /locus_tag="PKM27_17095"
     CDS             810..2042
                     /locus_tag="PKM27_17095"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09040.1"
                     /translation="MKGPAWMLAFWFLVSCGALQREYIRDPGQLVTEVKDAKFNKPNP
                     FFQADPLDFLPDTQHLSHSPRRYLRVNFHFMDAADSSRNFDETQSRKFVKDLLQVMQI
                     LFDTNIKLWLPQDNDIPVIPVNLKYVLSGTGPGDDGIYHHYDDQLYYYLHMGQRRNNA
                     DWAVIEKYAVGLDTTLNIFIMPHLPDSVKSGTYKSGCVGIALGNAIKIAGIYENKLTE
                     WNVRGTFNHEVGHVLGLYHAWTDDGCPDTQEHPNDCWVIEQAGCAGRTSNNMMDYNAW
                     QSALSPCQVGTIHKNLSDLRNPVRKLLVRTWCNYLPENKILIRDTIRWSGARDLESDI
                     EILPGGSLTIQGRVSMPGGSKILVHPGATLVLEDCLLHNDCDQAWEGIEVLKKGKKHG
                     LVIKSGQVEMRNVGGSGG"
     assembly_gap    2093..2192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2310..2960)
                     /locus_tag="PKM27_17100"
     CDS             complement(2310..2960)
                     /locus_tag="PKM27_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNR09041.1"
                     /translation="MIPYHLPYKSPEQMERASRAFYEHISQRRSIREFSNVPVPAKII
                     ENLILSASSAPSGANKQPWMFCAISNPALKSEIRKAAEKEERENYRGRMGDEWLKDLA
                     PLGTNEHKEFLETAPWLIAVFKKSYDFENRVKRKNYYVQESVGIACGFLLIAIYDAGL
                     AALTHTPSPMQFLKKILMRPENESPFLLIPVGYPAEGATVPDIRRKNAQEVIEWYL"
     gene            complement(3114..3821)
                     /locus_tag="PKM27_17105"
     CDS             complement(3114..3821)
                     /locus_tag="PKM27_17105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR09042.1"
                     /translation="MRPKAVITGATKGIGRAVAFKLVQEGYDLAVCSRNQQDLEDLKQ
                     ELHRLNPVSEVWIRTCDVSKPEQVKAWCGELSAHWSKLEILVNNAGLFYPGSVLTEPD
                     EALPHQLGVNLYSAYHFTRSLIPLIRRNASGHIFNLCSIASKTAYGNGGAYTISKFAV
                     YGFSQCLREELKPTDIKVTAVLPGATWSDSWKDSGHDPEEMIRPEDIAELIWTCSRLS
                     KVAVVEELVVRPQGGDL"
     gene            3993..4733
                     /locus_tag="PKM27_17110"
     CDS             3993..4733
                     /locus_tag="PKM27_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR09043.1"
                     /translation="MRLLEDLGRFILLFSHALAKPPNWRMYGKELIRQMYDIGNGSLI
                     IIALIAVFIGAVTAVQFLYQLDGTLIPTWYIGYIVRDSTIIELAPTISCLVLAGKVGS
                     NMASEIGGMRQKEHIDAMEVMGVNTASYLIMPKILAALLVIPLLVILAAFISIIGGYL
                     ASVLGGFMNTEEYTRGVRAFFIDRNLWMMLVKAGVFAFILTSVSCFQGYYVKGGSIEL
                     GKASTNAVVISNILILVSDYLIALMFTT"
     gene            4735..5499
                     /locus_tag="PKM27_17115"
     CDS             4735..5499
                     /locus_tag="PKM27_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR09044.1"
                     /translation="MIRANQLIKSFGEQQVIKEVSLEFETGKTNLVIGRSGSGKTVLL
                     KMLVGLIRPDSGSVLYDGEDFYSLDKKELQHLRMKVGMLFQGSALFDSMTVEENVRFP
                     MDMFTHLTKLEKAKRVDYCLERVGLKGNNKKFPSEVSGGMQKRVGIARAIVLNPQYLF
                     CDEPNSGLDPKTALLIDELIHDITVENNITTIINTHDMNSVMEIGETVAFLSNGVLCW
                     RGSKADILKSENDNLHDFIFASPFIQKLLEDADILK"
     gene            complement(6092..7585)
                     /locus_tag="PKM27_17120"
     CDS             complement(6092..7585)
                     /locus_tag="PKM27_17120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR09045.1"
                     /translation="MTARHHLLILFLTLLTGIATHWKIFPTDLIGVHLWRQAQNQWNI
                     RNFHRHDPNILNPRIAAHNLGHEGNILRYEFPLMQWSIAQAQEVCGESIRVTRISMFL
                     LGFLGILGFYQLAWILYKDRVLAACGAWMFSFSPLFYYYTLNPLSDIPALSSQIWMMV
                     FSLKYYRDKNFKDLVWAAVCLCLAGLFKLPFAVFGIMPWTAVLLRFYRDGKQWGPFIK
                     SHIPLLLASLPVLAWYGYAIASWQSMGVLKGIFGNTGFREILNYFTYHLFQWLPRHLL
                     NPAALLFFIAGLVLLLKKRDVRGGYAAILGAGAGMGLLYYAFEINMIARVHDYYLLPF
                     LPWLHLLAVHGLQAFQDHRALKTVSVFLLGGVPWIAWSLVNDYWSMDRNGYNPDWFIY
                     KNELKHAAPQDSLCILLNDNTGVVFAYAVDKQGYVFDRDELPPMWIEDMILRRRARYL
                     YSDSRKVDTSHQIRPFLDSLLMYKGSVKLFRLVDPVKMARAKKVEDK"
     gene            complement(7764..8858)
                     /locus_tag="PKM27_17125"
     CDS             complement(7764..8858)
                     /locus_tag="PKM27_17125"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNR09046.1"
                     /translation="MNKFFPIPLFSFIKIVFQNGGITAKGLLNTPPWLLKTILFEPLR
                     WIELVTKSKKIEKHSITKPPIFILGYCRSGTTYLQQLFMQDDRLGFTSNFQVVFPEIM
                     LSFEKKLTPALESITRFFKIQNPVYRMPMSWYFPGEEDVAMTTSLNPGGAQWGYFFPK
                     KMYEYYEKYVFFEYIPEAEIRAWKESYIFLLKKISLANQNKPLVLKSPPNTARIKFLL
                     SHFPGAKFIFIHRNPYEVYASNKRLWKVINDRYTLGTTRSVDFNTIILDTYSKIMDRY
                     LRDKDLIPQGQLAELSFEDLVYQPVESMRQIYESLDLDEYDYCENKMRSFAFLQKNHV
                     ILNHTLPLEETRMVSEKWGPFFRQWNYPLL"
     gene            complement(8908..10014)
                     /locus_tag="PKM27_17130"
     CDS             complement(8908..10014)
                     /locus_tag="PKM27_17130"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNR09047.1"
                     /translation="METLKKETIRIKYDQNEDHRFYNRMHDRIDEYFKSRNLSRYGDW
                     RIRLKIFTLFTIYFCSYFLLYRPGNTPGVILLYAVIFGLTGIMIALNIVHDASHNALF
                     KEKKWNQLLLYSMNLLGSLGYLYKLNHVKIHHTFPNVIGIDADLNQPNPLVRVSPGAP
                     KHKFHRYQHIYAPFIYMFYTLFLIFKKDFEDFKFIPKKDSPLLDVKHPKKEYFLFFTT
                     KIFYLTYALILPMIFLKIVWWKIVLGFLFVNVLMSIVAVGVQVLIHTNDKAHFIETDE
                     KGIIHRDWAVHSLINTSDLMAENDLVTFCLGGLNTHAVHHLFPGICHIHYPQLTKILK
                     QTAEEFGLPYTNLSLGESLRSHFVFLKQLGKDPV"
     gene            complement(10021..10758)
                     /locus_tag="PKM27_17135"
     CDS             complement(10021..10758)
                     /locus_tag="PKM27_17135"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNR09048.1"
                     /translation="MIPAGLSYLYFYVIRRDQWKPRHIQKRYPQTKSVLREIKWSFST
                     VLIITLITCIALFSIQEGKTQVYFSIVEYGWYYLFFSILLYIGLHDTYFYWIHRLLHF
                     KPVFRWVHKTHHLSHTPTPFASLSYNPLEAFLQFGINLILIFIIPLHPVAIGILLTHN
                     ILLNTGGHTGFEVMPRCFFHHWFFKYSLTTTHHDMHHTKLNCNYGLYFNIWDRIMGTN
                     HREYEKTFMEVSSEAPDPGKCLTYAGK"
     gene            complement(10821..>11013)
                     /locus_tag="PKM27_17140"
     CDS             complement(10821..>11013)
                     /locus_tag="PKM27_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09049.1"
                     /translation="KAFQEGEVNEDNKRRTCKKIPMLTKDKMLTLKALLGQNQVLPDK
                     CLAWNIMIGEYRHIADDCR"
BASE COUNT         2950 a         2513 c         2627 g         2823 t
ORIGIN      
        1 tgcaccttac caggaactca atgataaacc ctcttaacgt atctttgagc aaaagaaaac
       61 gagattatgc agttactgga aaacaaagta gccctgatca ccgggggtag ccgtgggatc
      121 ggagaagccc tggtcaggaa gtttgcctcg ctcggcgctt ccgtggcctt tacctattcc
      181 tcgtcctcgg aaaaagccac ggccctggcg gcctcccttg cccccgcgaa agtcatggct
      241 taccaatcgg acgcggcttc ctttacccag gctgaacaat tggtcaaaaa ggtattggcc
      301 gatttcggga aaattgatgt tttgatcaac aacgcgggca ttacccggga taccctgatg
      361 ctccgcatga ccgaagaaca atgggacgaa gtcatccggg taaacctgaa gtccgctttt
      421 aacctcacca aacaggtact caagcccatg cttggcgcaa gggccggttc catcatcaat
      481 atgacttccg tggtgggcgt tttcggcaac gcaggccagg ccaattacgc ggcgtccaaa
      541 gccggcatga tcgggtttac caaatccata gctaaagaac tggggagccg caacatccgc
      601 tgcaatgcca tcgctccggg atttatagaa accgacatga cgcatgccct ggatgataaa
      661 accagggaac tgtatttttc ctccctggcc attaaacggt tcggcaccgc cgaggaagtc
      721 gccgacctgg ccgccttcct cgcgggcgat acctccaaat acatcacggg ccaggtgatc
      781 agcatttgcg ggggactcaa tatgtgagca tgaaaggccc ggcctggatg cttgctttct
      841 ggtttctggt ttcctgtggg gccctgcaga gggagtatat cagggacccc ggtcagttgg
      901 tgacggaagt caaggacgcc aaattcaata aacccaatcc cttcttccag gccgatcccc
      961 tggacttcct tcccgatacc caacatcttt cccacagtcc acgacgttac ctccgggtta
     1021 attttcattt tatggatgcc gccgacagca gccgcaattt tgacgaaacc caaagccgga
     1081 aatttgtgaa ggaccttctg caggtcatgc agattttgtt tgataccaac atcaaactat
     1141 ggcttccgca ggacaacgac attccggtaa tcccggtcaa tttaaaatat gttctcagcg
     1201 gaaccgggcc gggggacgat gggatctatc accattacga cgatcaattg tattattatc
     1261 tccatatggg ccagcgcagg aacaatgcgg actgggcggt gattgaaaaa tatgccgtag
     1321 gcctggacac caccctcaat attttcatca tgccgcatct tcccgacagc gtgaagtcag
     1381 gaacgtataa atccggttgc gtgggcattg ccctgggcaa cgccataaaa attgccggca
     1441 tttacgaaaa caaactgacc gaatggaatg tgcggggcac cttcaaccat gaagtgggac
     1501 atgtgttggg tctctatcat gcctggaccg acgacggttg cccggatacc caggaacacc
     1561 ccaacgattg ttgggtcatc gaacaagccg gatgcgccgg ccgcacctca aacaacatga
     1621 tggactacaa tgcctggcaa agcgcactgt ccccctgcca ggtcggtacg attcataaaa
     1681 atctttcgga tctccggaat ccggtgcgga aattactggt caggacctgg tgcaattacc
     1741 tgcctgaaaa caaaatcctg atccgggata ccatccgttg gagtggcgcc cgcgacctgg
     1801 aaagcgatat cgaaatcctg ccgggtgggt ccctgactat ccagggcagg gtctccatgc
     1861 ctggaggatc aaagatcctg gtccatcccg gcgccaccct ggtcctggag gattgcctct
     1921 tgcacaacga ttgcgatcag gcctgggaag gaatcgaagt cttgaaaaaa ggcaaaaaac
     1981 acggattggt gatcaaatcg ggacaagttg aaatgaggaa tgtgggtggg agcggaggtt
     2041 agaggtatga ggaaagaggt tagaggtcag tccactcgct cctcgcacct cannnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttagagg tatgaggaaa gaggttagag
     2221 gtcagtccac tcgctcctcg cacctcatac ctcaaacctc acacctcaca cctcaaacct
     2281 cacacctcac acctcacacc tcacacctac taaaggtacc attcaatcac ctcctgcgca
     2341 ttttttcttc tgatatccgg gacggtggct ccctccgccg gatagcccac ggggatcagc
     2401 aggaaggggc tctcgttttc cggccgcatt aatatttttt ttaaaaactg catggggctg
     2461 ggggtatggg tgagggcggc cagtcctgca tcgtatattg cgatcagtaa aaacccgcag
     2521 gcgataccca cggattcctg tacgtaataa ttttttcttt ttaccctgtt ttcgaaatca
     2581 tacgattttt taaagacggc aatgagccag ggtgccgtct ccaggaactc tttatgttca
     2641 ttcgtaccca gaggggccag gtccttcagc cattcatcac ccatccggcc ccgataattt
     2701 tcccgttctt ctttttccgc tgctttacgg atctccgatt ttaaagcggg attggatatc
     2761 gcacaaaaca tccagggctg cttgttagcc cccgatggcg ccgaagatgc tgaaagaatg
     2821 aggttttcaa taattttagc aggtaccggg acatttgaaa attcccgaat ggatcgcctt
     2881 tgactgatgt gctcgtaaaa ggcacggctg gcccgctcca tttgttccgg ggatttgtaa
     2941 gggagatggt agggaatcat ggtagaagtt agaagctgga agttagaaat tggaagccag
     3001 gagaacggca ctccccttta tctattgatt actgacctta gaatatatag gttaagggat
     3061 aacggttaag ggttaaagga ccacaattac tactgactac tgactactga ccactacaaa
     3121 tcccctccct gcggccggac caccagttct tccactacgg ccaccttgct caaccggctg
     3181 caggtccaga tcaattccgc gatgtcctcg gggcggatca tttcttccgg atcatgtccc
     3241 gaatctttcc agctgtccga ccaggtggcg ccgggcaata cggcggtaac tttgatatcc
     3301 gtgggtttga gttcttcgcg caggcattgg gaaaagccat aaaccgcaaa cttggagatg
     3361 gtataagcgc ccccgttgcc gtatgcggtc ttgctggcga tggaacagag attaaagata
     3421 tgcccgctgg cgttccgccg gatcaggggg atcagggacc gggtaaaatg ataggcgctg
     3481 tataggttaa ccccgagctg gtggggcagg gcttcatccg gctcggtcaa aacggaaccg
     3541 ggataaaaaa ggccggcatt gttgacgagg atttccagtt ttgaccaatg ggccgacaat
     3601 tctccgcacc aggcttttac ttgttccggt ttgctaacat cgcaggtccg gatccatact
     3661 tccgatacag ggttgagacg gtgcaactct tgtttcaggt cttccaggtc ttgctggttt
     3721 cgggaacaaa ccgccagatc atacccttcc tgaaccagtt taaaagcgac cgccctgcca
     3781 atgcctttgg tagccccggt gatcacagcc ttaggcctca tgatgatttt tttcaacaag
     3841 gtaaatattt aagcgattcc gggcccggtc cgggccaaaa acaggggggc tcgaagagag
     3901 ggggtgtctc gaagagaggg ggtgtctcga agacaccccc tctctttttg gttcaccctc
     3961 attccttatc tttgcggttt cggagtccat tcatgcgttt attggaagat cttggccggt
     4021 ttattttgtt gttcagccat gctttggcca aaccgcccaa ctggcgcatg tacggcaaag
     4081 agctcattcg ccagatgtac gacatcggca acggatcgct catcatcatt gcgctgattg
     4141 ccgtttttat cggagcggtg acggccgtgc agttcctgta ccagttggat ggcaccctga
     4201 tacctacctg gtacattgga tatatcgtcc gggactccac catcatcgaa ctggcgccga
     4261 cgatcagttg cctggtcctg gcgggcaaag tagggtccaa tatggcctcg gagatcgggg
     4321 gcatgcgcca gaaggaacat atcgatgcca tggaagtgat gggggtcaat accgcttctt
     4381 acctgatcat gccaaaaatc ctggcggctt tgctggtcat ccccctcctg gtgatcctcg
     4441 ccgccttcat cagcatcatt ggaggttacc tggccagtgt gctgggtggt tttatgaata
     4501 ccgaggaata tacccggggc gtgcgcgctt tttttattga ccggaacttg tggatgatgc
     4561 tggtcaaagc cggggtgttc gcttttatcc tcacttcggt atcctgtttc cagggatatt
     4621 acgtcaaggg aggaagcatc gagttgggca aggccagcac caacgccgtg gtcatcagca
     4681 atattcttat cctggtctcc gattacctca tcgccttaat gtttaccacc tgatatgata
     4741 cgcgccaatc agctcattaa atctttcggt gaacagcagg tgatcaagga ggtttccctg
     4801 gagtttgaaa ccgggaaaac caacctggtg atcggccgga gcggaagcgg caagaccgta
     4861 ttgctcaaaa tgctggtggg tttgatccgg cccgacagcg gctccgtttt gtacgacggg
     4921 gaggattttt acagcctgga caaaaaggaa ttacagcatt tgcgcatgaa agtgggcatg
     4981 ctgttccagg gttctgcctt gtttgattcc atgaccgtgg aagaaaatgt ccggtttccc
     5041 atggacatgt ttacccacct gaccaagctt gaaaaagcta agcgggtcga ttattgcctg
     5101 gagcgggtcg ggctcaaggg caacaataaa aaattccctt ccgaggtcag cggagggatg
     5161 cagaaaagag ttggcattgc ccgtgccatt gtcctcaatc cacaatatct tttttgtgac
     5221 gagcccaaca gcgggctgga tccaaagacg gccctcctga tcgatgagtt gatccacgat
     5281 atcacggtgg aaaacaacat cacgaccatc atcaataccc atgatatgaa ttcggtgatg
     5341 gagatcggcg aaaccgttgc ctttttaagc aatggggtcc tctgctggcg gggttcaaaa
     5401 gcggatatcc ttaaaagcga gaatgacaat ctgcacgatt ttatttttgc ctctcctttt
     5461 atacaaaaat tacttgaaga cgcggatatc ctgaaataaa aaacccggac ttgatcaaca
     5521 aatccgggca gaagactaag gtctcaaaat aagaaaacta catctttata acgaataccg
     5581 ggtgaaaatt gaaccccggt ttctattgat atgcttgttt tgtcctgctt ccaggaatcc
     5641 ggctgattga gaataaaatt ctgattatca accgttaata ttttaaatat agcaaaaatt
     5701 ctgccaaact ttcaatgact ttaactaaat cttacatata ttttctataa aaatttatat
     5761 attcttttta atcaacgtaa tacaatttaa atattgcttt aaagtatttt tttaaaagtt
     5821 aagaaaatac atattatact ttccttgata tatatttaaa ttcaactttt gctttaactt
     5881 aaagtgctta ccggggaaat aaaagctaat ccggtcatag tcagtgatat aaatttaaag
     5941 ggttgaatcg gccggccgga tggacttttt gaggtataaa tttatatttt accaatagtc
     6001 catttttatc tttcgacaaa tacacaggca ttgcttatgc catccggcca accaaaagat
     6061 cgtctggata gggatgcact cgcaactgat tttatttatc ctccactttt tttgcccgag
     6121 ccatcttcac cgggtcgacc agtcggaata gttttaccga tcctttatac atcagcaggc
     6181 tatccagaaa tggcctgatc tggtggctgg tgtcgacctt gcggctgtcc gaatacagat
     6241 acctggccct ccttcggaga atcatgtctt ctatccacat tgggggcaat tcatcccggt
     6301 caaacacata gccctgtttg tcgaccgcat aagcaaagac cacgccggtg ttgtcattga
     6361 gtaaaatgca taggctgtcc tgcggcgccg catgtttcaa ttcgttttta taaataaacc
     6421 aatccgggtt gtacccgttg cggtccatac tccaataatc gttgaccaga gaccaggcga
     6481 tccaagggac ccctccgaga agaaaaaccg aaaccgtctt taatgcccgg tggtcctgga
     6541 aagcttgcag accatgaacc gcaagcagat gcagccaggg aaggaaaggc aacaaataat
     6601 aatcatgcac cctggcaatc atattgattt cgaaagcata atacaataaa cccatacctg
     6661 cccccgcgcc gaggatggcc gcgtatccgc cccgaacatc cctttttttc aagagcagga
     6721 ctaggccggc aataaaaaac aacagggcag cggggttgag cagatgacgc ggcagccatt
     6781 gaaacaggtg ataagtgaaa taattcagga tctcccggaa cccggtattt ccgaaaatgc
     6841 ctttcaacac ccccatactt tgccaggaag ctatggcata tccataccag gccaggacag
     6901 gcagggaggc gaggagcaag gggatatgcg atttaatgaa gggtccccat tgttttccgt
     6961 cccggtaaaa ccggagaagc acggccgtcc acggcatgat gccaaaaacg gcaaagggca
     7021 atttaaacag cccggcaagg cagaggcata ccgcggccca taccaggtct ttaaaatttt
     7081 tatcccggta atatttcagg gaaaacacca tcatccagat ttgtgaagac agggcgggaa
     7141 tgtcggatag gggattgagg gtataataat aaaacaaggg agaaaaactg aacatccagg
     7201 caccgcaggc tgccaggacc cggtccttgt aaaggatcca agccaattgg tagaatccca
     7261 ggatccccag gaaaccaagc aggaacatgc tgatccgcgt gacccgaatc gattctccgc
     7321 agacctcttg cgcctgggca atagaccatt gcatcagcgg aaattcataa cgcaggatat
     7381 tgccctcatg ccccaggttg tgcgcagcaa tcctgggatt gaggatattg gggtcgtgac
     7441 ggtggaaatt gcggatattc cactggttct gggcttgccg ccagaggtgg acgccgatca
     7501 ggtcggttgg aaagatcttc caatgggtgg caatgccggt gagcagggtg agaaagagga
     7561 ttaggaggtg atggcgggcg gtcatgggcg ggagttatcg tgcactttac cgggcgttat
     7621 ttaaaatgat tttatttgtt taaggtattc gttgaaaaca aatatgtttc cctgaagcac
     7681 ggatccaggg cattgccctc ctgctcgccc tgagccgatc catttgggtt tttatcagca
     7741 taaggcctga gtataggatg aaattataaa agcgggtaat tccattgcct gaaaaaaggt
     7801 ccccattttt cggaaaccat tcttgtttcc tccagtggca aggtatgatt taatataaca
     7861 tgattttttt ggaggaatgc aaaggacctc attttatttt cacaataatc atattcatcc
     7921 agatcaagtg attcatagat ctgcctcatg ctttccacag gctggtatac caaatcttca
     7981 aatgacaatt cagctaattg gccctgggga atcaaatcct tgtcccgcag gtaacggtcc
     8041 atgatttttg aataggtatc caaaataatg gtattgaaat caacagaccg ggtggttcct
     8101 aaagtatacc tatcgtttat taccttccat aatcgtttat tggacgcata cacttcatag
     8161 ggattccggt ggataaaaat aaattttgcc ccggggaaat gggaaagtaa aaactttatc
     8221 cgtgccgtat tgggaggact tttcaggacc agtggtttgt tttgattggc gagtgaaatt
     8281 tttttaagca ggaatatata ggactctttc caggcccgga tttcagcctc ggggatatat
     8341 tcaaaaaaga catatttttc ataatattca tacatctttt tcgggaaaaa ataaccccat
     8401 tgcgcccctc ccggatttaa cgaggtggtc atagctacgt cttcttcgcc cgggaaatac
     8461 caggacatcg gcattcgata tacagggttt tgaattttaa aaaaccgggt tatggattcc
     8521 agggcggggg ttaatttttt ttcaaaactc aacatgattt cgggaaacac cacctggaaa
     8581 tttgaagtga atcccaggcg atcatcctgc atgaataatt gctgaagata agtggtgccg
     8641 ctgcgacagt aaccgagaat aaaaatcggt ggttttgtta tgctatgttt ttcgattttt
     8701 ttactttttg taaccaactc aatccagcgt agcggttcaa acaaaatggt tttaagtaac
     8761 caggggggag tatttaacaa gcctttagcg gtaatcccgc cattttgaaa tacgatctta
     8821 ataaaggaaa aaagggggat cgggaaaaac ttgttcatat tgctgaaaat gcggaaacag
     8881 taggtatgga accgatggga tctggtctta taccggatct ttcccaagtt gctttaaaaa
     8941 tacaaaatgg gacctgagtg attcacctag agaaagattg gtatagggga ggccgaattc
     9001 ttctgctgtt tgcttcagga ttttagtcaa ttgaggataa tggatatggc aaattccagg
     9061 gaataaatga tggacggcat gggtgtttaa tcccccaaga caaaaggtga ccaggtcgtt
     9121 ttccgccatg aggtctgaag tattgatcag ggaatgtacc gcccaatccc ggtgtatgat
     9181 ccctttttca tccgtttcta tgaagtgtgc tttatcgttc gtatgaatca gaacctggac
     9241 cccgaccgca acgatgctca tgagcacatt tacaaataaa aaccctaaaa ctattttcca
     9301 ccatactatt ttcaggaaga tcataggcag gatgagcgcg taggtcaggt aaaatatttt
     9361 tgttgtaaag aacaggaagt actctttttt gggatgtttt acatccagca aagggctgtc
     9421 ttttttcgga atgaacttaa agtcttcgaa atccttttta aatatcagga aaagggtgta
     9481 aaacatatat atgaatggag cataaatatg ctggtagcgg tgaaatttat gttttggagc
     9541 tcccggagaa acccggacca acggattggg ctggttgaga tccgcatcga tgccaattac
     9601 attggggaag gtatgatgaa ttttaacgtg atttaattta tacagataac ccaaactgcc
     9661 taacagattc atcgaataca acaacaattg gttccatttt ttttccttga atagggcgtt
     9721 gtgggaagca tcgtgaacaa tatttagagc gatcattatg ccggtcagtc cgaaaatgac
     9781 cgcatagagc aggatcacac cgggagtgtt gcctggccgg tacaacagga aataggagca
     9841 aaaatagatg gtaaacaagg tgaatatttt cagacggatt ctccagtctc cgtagcggga
     9901 cagattgcga ctcttaaaat attcatcaat ccggtcatgc atcctgttat aaaagcggtg
     9961 atcctcgttt tggtcgtatt ttatccggat tgtttccttt ttcagggtct ccataaataa
    10021 ttattttcca gcataagtta aacattttcc ggggtccggg gcttcggaac tgacctccat
    10081 aaaggtcttt tcatattcac ggtgattggt gcccatgatc cggtcccaaa tattaaaata
    10141 aaggccgtaa ttgcagttta atttggtatg atgcatatcg tggtgcgtgg tggtaaggct
    10201 gtatttaaag aaccaatgat gaaaaaaaca gcgtggcatc acctcgaagc ccgtatggcc
    10261 cccagtgttc agcaggatat tatgagtcaa taaaatacca attgcaacag gatgaagggg
    10321 tatgataaaa atcaatatca aattgatccc gaattgaagg aatgcttcca atgggttata
    10381 agatagtgaa gcgaaagggg tcggcgtatg agaaaggtgg tgcgttttgt gtacccagcg
    10441 aaatacaggt ttaaagtgca gcaaccggtg gatccaatag aaataagtat cgtgtaaacc
    10501 aatatataaa agaatgctga aaaacagata ataccaacca tactccacaa tcgaaaaata
    10561 cacttgtgtt ttaccctctt gaatggaaaa aagggcgatg caggttatta gagttattat
    10621 gagaacagtg ctgaatgacc attttatttc ccttaaaacc gatttggttt gaggatatct
    10681 tttttgaatg tgcctgggct tccattgatc acgccggatc acataaaaat agagataact
    10741 aagccctgca ggaatcatat aatacccaaa gagaatgatg caaaagacag acaaaatgaa
    10801 tggtatggtt tcaaatgcaa tcatctgcaa tcatctgcaa tgtgtctgta ttcaccgatc
    10861 attatattcc aagcaaggca tttatcgggc aaaacttgat tttgcccaag caaggctttt
    10921 aaagttagca ttttatcttt agttagcata gggatctttt tgcaagtcct tcgcttgtta
    10981 tcctcattga cttctccttc ttgaaaggcc ttt
//
LOCUS       DAOQXT010000132        10967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6414_length_10967_cov_6.809201, whole genome shotgun sequence.
ACCESSION   DAOQXT010000132 DAOQXT010000000
VERSION     DAOQXT010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_10967_cov_6.809201"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PKM27_17145"
     CDS             complement(<1..782)
                     /locus_tag="PKM27_17145"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNR09050.1"
                     /translation="MIDLPVTTDPTPARPKKPDWLRVKLPIGENYRMVRELVDTHKLH
                     TICQSGNCPNMGECWGEGTATFMILGNVCTRSCSFCAVKTGRPTEYDTDEPERVAMAI
                     SLMKVKHAVITSVNRDELADKGAEIWHRTVKAVKTLSPGTTIETLIPDTKADWKALEM
                     MISPGQEVVSHNMETVKRLYRPVRPQAKYERSLEQIARTKAYGKRTKSGVMLGLGETR
                     DEVIQILEDLAAHGCDVLTLGQYLQPTRLHHPVMEFVHPDQFA"
     gene            complement(898..3090)
                     /locus_tag="PKM27_17150"
     CDS             complement(898..3090)
                     /locus_tag="PKM27_17150"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNR09051.1"
                     /translation="MIRPLTLLPSFLLFIPCFVFAFEPAPAELRKWKEQAARVTIIRD
                     SFGIPHIYGRTDADAVFGLLYAQCEDDFKRVELNYIEKLGRLAELRGESSLFDDLYIR
                     MILDQDAAKKDYKNSPAWLRKLLHAFADGINYYLYRHPETRPALIQRFEPWYPLLWTD
                     GSIGAINTADVGPEDLKNFYTSGPMGLTHEKAENQGGGSNGFAIAPARSVSGNAMLYI
                     NPHVTFYFRPEVHMVSEEGLNAYGAVTWGQFFVYQGFNPHCGWMHTSCNVDVADVYLE
                     TTRPKGQGWTYRYEKKERPVTKKPISLKFKDGILVRTRTFDTWSTHHGPVMATRDGHW
                     ATVRSNNRSLDGLKQSWLRTKAKGFEEFKKTMDLRSNISNNTVYADAGGHIAYWHGNF
                     IPRRAAGINWSKPVDGSTGTTEWQGLHTVGESVHLYDPPNGWLQNCNSTPFTAAGPES
                     PSKANFPPYMAPDGENFRGINAVRVLSGRGKFDLDGLIRAGYDTYLSAFEVLVPALVE
                     AFENYGHGGDNKFDALEGPVNLLKNWDYRSGVSSVETTLATEWAQRLGPSIEKVYIDE
                     GEDDQVSRTRAFARSATEDQLLQPLLSAVEYLTNTYGTWETPWGDINRFQRLSGEVIQ
                     KPDDAKPSLPVGFASASWGMLPSYNSRRFEGTRKRYGVSGNSFICAVEFGPKIRAKSL
                     LAGGQSGDPASPHFKDQAERYTTGTFKEVLFYREEVEKQATKKYRPGE"
     gene            complement(3185..6211)
                     /locus_tag="PKM27_17155"
     CDS             complement(3185..6211)
                     /locus_tag="PKM27_17155"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNR09052.1"
                     /translation="MDTFRKQSNIAGWLVFGLAFLVYYLTAERMGSLWDCGEFILGAA
                     KLQVVHPPGAPLFILVGRLFAWVASLISSEPSAQAFAINLLSGICTAFAVAFVCWSTI
                     MLSKLAWLGKDQEPDAHQGLVLSAAGLVAGLTGAFCTSVWFSAVEGEVYAMSTFFTAL
                     TVWAGLKWYYLPSSAQNEKWLILALFVAALSIGVHLLSLLTFPVIGLLYYYKRFQKHD
                     LRGAFFALAGSIVVLGLYQKLVIVGLPKLWSLFDYMMVNGLGMPFHTGIFPFILVIGA
                     GIYYGLHLARKNGSAMLQNIVLTSLLVILGFSTVAVVVIRANANPPINMNDPSDAMRL
                     IPYLNREQYGDRPLLRGPAYNASAIDYTSEDRYGRVGDRYEVVDKKFTPIYRSSDQKF
                     FPRMGHSDRASLYQYWMNGKAKPEQADNVSFFIRYQVMWMYWRYFMWNFAGRQNFDQG
                     YFPWNLKSGHWFTGIKPIDELRLYNLDKEPSRMKNDPGRNRYFFIPLILGILGLLWHY
                     KKAKKEFIALLMLFVITGLGIIIYSNQPPNEPRERDYVLVGSFLTFAIWVGMGVIYLY
                     QFISDKLKGNKAVPLALAGGLGLLSPLVMVTQGFDDHSRRHLSGSRDYALNFLESCAP
                     NSILFTYGDNDTYPLWYAQEMEGIRTDVRVINLSLIAVDWYINQVRRKINLSDPVKLS
                     ISEENYRGDSRMQLVINAPENTTRNVNDVLKFINERHPLPLQSGQTMETYVPARKILI
                     PIDRNRALASGLISPTDTNIASQIEINLTGSYIMKDDLAVLDIIASNIYDRPIYFATT
                     ANPDRLIGLNNYSRLEGMAARIVPILTQPDGRFGQYGSGGVDGNRIYENVLKKFQWGG
                     FDKYQMFNFENSFNPSFSAMRIAFMRGIDRLIKDGEMEKAKSLTDKYFEVFPQKNFEY
                     NSQRLVFIEFYFQLSALDRVKEESLKLAAETAEYLEFYNSLSPEDLESGFQEDYNQYM
                     TVYPRLTTLAKEMQDPEFEKQINDILGKFSTAPVKK"
     gene            6305..6463
                     /locus_tag="PKM27_17160"
     CDS             6305..6463
                     /locus_tag="PKM27_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09053.1"
                     /translation="MLKLLLLAAIGYIVYRYVWRDPSIDKAQPRKPLRDSPFANDEIE
                     DTEYTEKK"
     gene            6460..7137
                     /locus_tag="PKM27_17165"
     CDS             6460..7137
                     /locus_tag="PKM27_17165"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNR09054.1"
                     /translation="MNPTGLKDGIIQSNHHFIVYNKPAGMAVQSVHHKEESLESKLAH
                     YCKTKLFLIHRIDQPCSGLVLFTKKKTGAGLINEQLKNQEVERIYLAVVQTKPEPEAG
                     TLHHFLFAHKKSNKSFVVDEGHKEGKAAQLHYQWLGTSGGNHLLMIRLKSGRHHQIRA
                     QLAAIGSPIKGDVKYGAKKYNEDGRIHLHAWKLKFSHPANGSPVSFEAPIPEEATWQS
                     LTAFVPS"
     gene            7167..8198
                     /gene="thiL"
                     /locus_tag="PKM27_17170"
     CDS             7167..8198
                     /gene="thiL"
                     /locus_tag="PKM27_17170"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNR09055.1"
                     /translation="MEEKKTDVNTLGEFGLIDHLTRNTVRYHRETLCGVGDDAAVLEG
                     SDQLTLVTTDLLIEGIHFDLMYTPLKHLGYKSVVVNVSDLCAMNGKARQITTSIALSN
                     RFSVEALDQIYEGIHLACKHYEVDLIGGDTSSSPKGLYLSSTAIGQVAPDQVVYRSGA
                     RPRDLICVTGDLGSAYLGLQLLEREKQIFQSAPGIQPELNEKYSPLIGRQLKPEARRD
                     VVESFHKLHVKPHAMIDISDGLSSEIMHICKQSGTGALIEENLVPIHPLATELALEFN
                     LNPITCALNGGEDYELLFTISEKEFERIKYLPGFYVIGEIRPAEEKILLRTSGGKYVP
                     LEAQGWRHV"
     gene            8320..9714
                     /locus_tag="PKM27_17175"
     CDS             8320..9714
                     /locus_tag="PKM27_17175"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HNR09056.1"
                     /translation="MKIIRIVFFTVIALNPAVGQTFFFGNDLSYVNEMEDCGVIYKEN
                     KKAKDPYRIFADRGCNLVRLRLWKDPSWYKSLNQGKIYSDLGDVMKSIGRANANQMQV
                     LLDFHLSDNWADPSKQLVPAAWEPVVNNLPVLKDSLYRYIYNTLQILFQADLLPEMVQ
                     IGNETNKGILLSPADNAKYTLDWPRNAELFNAAIAAVRDFEKSSKQKIKVAIHAAGPK
                     DSGWLIDQFVQNGVTDFDLIGISYYWAWHKPSSIEETGALIQSLKNKYRKEVMIFETG
                     YLWTTASQDQANNIISEVHPDYAPASPENQKKWLIDLTKEVMLKGGSGVIYWEPAWVS
                     SPCFTQWGQGSHQEHAAFFDFEHDLIEEGGIGWMSYDYLSTVPLESDHTLNGIRVVVD
                     SLAKKLQIETAGPETSIRRIRIYDMKGSLVLAESPGEAGRVYTLSLQSLGSSLYILEV
                     WDQSNKSFRQKIML"
     gene            9838..>10967
                     /locus_tag="PKM27_17180"
     CDS             9838..>10967
                     /locus_tag="PKM27_17180"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNR09057.1"
                     /translation="MKKLLLLALTLFGFTLADAQRTVTGTVFDNSTNEPLIGASVQVE
                     GSNQGVITDIDGSYSIQVPDGAVRLIVSYVGFTTRIVEVGNASTLDIVLEPGQQLQEI
                     VVIGYGTVKREDATGSIQVVTSEKFNKGAITAAPELLTGKIAGLQVNNSSEPGGGSVI
                     RIRGGSSLSASNDPLIVIDGVPVANDGISGDRNVLNIVNPNDIESVTVLKDASATAIY
                     GSRASNGVILITTKKSRTTQKIAVDYSGSYALSNRINEVEVLSADEFRTYVRTRYPDS
                     HPAPALLGNANTDWQDLIFQKGQTLDQNLSLSGRVGILPYRFSVGYTDRTGLLKTDKF
                     NRKTAALKLTPKFINNTLQVIFDVKGMLINNRFADWGAIGSAT"
BASE COUNT         2689 a         2874 c         2852 g         2552 t
ORIGIN      
        1 gcaaactggt ccggatggac aaattccatc acgggatgat gcagtcgggt cggttgcagg
       61 tattggccga gggtcaatac atcgcaacca tgtgcagcga ggtcctccaa gatttggatc
      121 acttcgtccc gggtctcgcc caggcccagc atgacgccgc ttttggtgcg ttttccatag
      181 gctttggtgc gggcgatctg ctcgagggac cgttcatact ttgcctgggg ccttaccggt
      241 cgatagagtc ttttgacggt ttccatattg tgggaaacca cttcctggcc gggactgatc
      301 atcatttcaa gagccttcca gtcggctttg gtatccggga tcagggtttc aatggtggtt
      361 cccggggaaa gggttttcac ggctttaacg gtacggtgcc agatttcagc ccctttatca
      421 gccaattcat cccggttgac actggtgatc accgcgtgct tcaccttcat caaggaaatg
      481 gccatcgcca cccgttctgg ttcatcggtg tcatattcgg tggggcgccc ggttttgacc
      541 gcgcaaaaac tgcacgagcg ggtgcacaca tttccaagga tcatgaaggt ggccgtgcct
      601 tcgccccagc attctcccat attggggcag ttgccgctct gacagatggt atgcagttta
      661 tgagtgtcca ccaactccct gaccatgcgg tagttttccc cgatgggcag ttttacgcgc
      721 agccaatccg gttttttggg cctggccggt gtaggatccg tggtgactgg caaatcaatc
      781 atgagggttc aaagataagg cggattggta gagcgatccc tatgattctt gcctccgaat
      841 cttcaagggc gtgaaccagg agggtgcaag atcccgtatt catgccacag gtcaaaacta
      901 ttctcccggc ctgtacttct ttgttgcctg cttttccacc tcttcccggt aaaacaggac
      961 ttctttaaac gtacctgtgg tatacctctc cgcctggtct ttgaaatgag gggaagcagg
     1021 gtcgccgctt tgcccgccgg ccaggaggga cttggccctg attttcgggc cgaattccac
     1081 ggcgcagata aaactgttgc cgctcactcc atacctcttc cgggtgcctt caaatctccg
     1141 gctgttgtag gatggcagca tcccccagga agcggatgca aaaccaacgg gaaggcttgg
     1201 ttttgcatca tccggttttt gtatgacctc acccgataag cgctggaacc ggttgatatc
     1261 cccccagggg gtttcccagg ttccataggt attggtaaga tattccacgg cactgagtaa
     1321 aggttgcaac aattggtcct ccgtcgcgga acgcgcaaaa gccctggtgc ggctgacctg
     1381 gtcgtcttcg ccttcatcga tatagacttt ctcgatggag ggtccaagtc tctgggccca
     1441 ttccgtggcc agggtggttt ccaccgagga cacgccgctg cggtaatccc aattttttaa
     1501 aaggtttacc ggtccttcca gggcatcgaa tttattgtct cccccgtggc cataattttc
     1561 aaatgcttcc accagggctg ggaccaatac ttcaaaagca gaaagatagg tatcgtaacc
     1621 ggcccggatc aggccgtcga ggtcaaattt tccccggccg gacaagaccc ttaccgcgtt
     1681 gattccacgg aagttttccc cgtcgggggc catgtagggc ggaaagttgg ctttagacgg
     1741 gctttccggc cctgccgcgg tgaatggggt ggaattgcaa ttttgcagcc agccgttcgg
     1801 ggggtcatag aggtggacgc tctctccgac cgtgtgcagg ccttgccatt ccgtagtccc
     1861 ggtacttcca tccaccggtt tgctccagtt gatgccggct gcccgtcttg gaataaaatt
     1921 gccatgccaa taggcaatat gaccgccggc atccgcatac acggtattgt ttgagatatt
     1981 gcttcgcagg tccatggtct ttttaaattc ctcaaatcct ttcgccttgg tccggagcca
     2041 gctctgtttc agtccgtcga gtgagcggtt gttggaacgg accgtggccc aatgtccgtc
     2101 gcgggtcgcc atgaccgggc catgatgggt agaccaggtg tcgaaggttc tggtgcggac
     2161 caggatcccg tctttaaatt tcagcgaaat cggttttttc gtgacgggcc tttccttttt
     2221 ttcataccgg taagtccagc cttggccctt tggacgggtg gtttccaggt agacatcggc
     2281 gacgtccaca ttacagctgg tgtgcatcca gccgcaatgc gggttgaagc cctggtagac
     2341 gaaaaactgt ccccaggtga ccgcgccata ggcattcaat ccttcttcgc tgaccatatg
     2401 gacttccggc cggaaataaa aagtgacatg agggttgata taaagcatgg cgttaccgga
     2461 aacggaacgg gccggggcaa tcgcaaaacc gtttgagccg ccgccctggt tttccgcttt
     2521 ttcatgggtc aggcccatag gtccggaggt ataaaaattt ttaagatcct ccggcccgac
     2581 atcggccgta ttgatggccc ctatgcttcc gtctgtccag agaagggggt accagggttc
     2641 aaaacgctgg atgagtgcgg gacgggtttc cgggtgtcgg tataaataat aattgatgcc
     2701 gtccgcaaaa gcatggagca gtttcctgag ccaggcgggg ctgtttttat aatccttttt
     2761 tgcagcatcc tggtcgagaa tcatccggat ataaaggtca tcaaacaggg aggattctcc
     2821 gcgcagttcg gccaggcgtc ccagtttttc aatataattc agttcgaccc gtttaaaatc
     2881 gtcttcgcat tgggcgtaaa ggaggccaaa gacggcatcg gcatccgtcc tgccatagat
     2941 atgcggaatg ccgaaagagt cacggatgat ggttacgcgg gcagcttgtt ccttccattt
     3001 cctgagctca gccggcgccg gttcaaaggc aaaaacaaaa caagggatga acaggaggaa
     3061 ggatgggagc agggtcaggg gccggatcat caccaaaaat atcatttaag gccaggatcc
     3121 cgtaaataaa aaaaccggct ggcccaaaaa gagtccaacc ggtttctgta tcgattaaag
     3181 tcttctattt ttttaccggg gcggttgaaa acttacccag aatatcgttg atctgttttt
     3241 caaattccgg gtcctgcatt tctttggcca gggtggtcag ccggggatag accgtcatgt
     3301 attgattgta atcttcctgg aaaccggact ccagatcttc gggggacagg gaattgtaga
     3361 actccagata ctctgctgtt tcagcagcca gtttcaggct ctcttccttc acgcggtcca
     3421 gggcgctcag ctgaaaataa aactcgataa agaccaggcg ctgggagttg tattcaaagt
     3481 ttttctgggg gaatacctca aaatatttgt cggtcaggct ttttgctttt tccatttccc
     3541 cgtctttgat cagcctgtcg atcccgcgca tgaaggcgat ccgcatggca gaaaaactgg
     3601 ggttgaagct gttctcgaaa ttgaacatct ggtatttgtc aaaacctccc cactggaatt
     3661 ttttgagcac attttcatag atcctgttgc cgtccacccc accggatcca tattgtccga
     3721 aacgtccgtc gggttgggtc aggatgggaa cgatccgtgc ggccatgcct tccaaccttg
     3781 aataattatt gaggccgatc agccggtcgg ggtttgccgt ggtggcaaaa tagatcgggc
     3841 ggtcatagat gttggaggca atgatatcca gaacggccag gtcatccttc atgatataac
     3901 tgccggtgag gttgatttcg atctggctgg caatgttggt atcggtggga ctgatcaggc
     3961 ctgaagccag ggccctgttc cggtcgatgg ggatcaggat tttccgggca ggcacatagg
     4021 tttccatggt ctggccgctc tgaagcggaa gggggtgacg ttcgttgatg aattttaaaa
     4081 catcatttac gtttctcgtg gtgttttcgg gcgcgttgat caccaattgc atccggctgt
     4141 ccccgcggta attttcttca gaaatcgaaa gtttgacggg atcggaaagg ttgattttcc
     4201 ttctcacctg gttgatatac cagtcgaccg cgataaggct caggttgatc acccggacat
     4261 ccgtgcggat gccttccatt tcctgggcat accagagggg gtaggtatcg ttgtccccat
     4321 aggtaaacag gatcgaattg ggggcgcagg attcaagaaa gttgagggca taatcgcgtg
     4381 aaccgctgag atgcctgcgt gagtggtcgt caaaaccctg ggtgaccatg accagggggc
     4441 tcagcaaacc aaggcctccg gccagggcca gcggaacggc tttattcccc ttgagtttat
     4501 ccgaaatgaa ctggtacaga tatatgaccc ccatccccac ccagatggcg aaagtgagga
     4561 aggaacctac caggacataa tccctttcac ggggttcatt gggcggctgg ttggaataaa
     4621 taataatgcc taaaccggtg atgacaaaaa gcatcagcag ggcgatgaat tcctttttcg
     4681 cttttttata atgccagagc aggcccagga tgcccaggat cagcgggatg aaaaaatacc
     4741 ggttcctgcc cggatcgttt ttcatccggc tgggctcctt gtccaggttg taaagccgga
     4801 gctcatcgat gggtttgatc cctgtgaacc aatgcccgct tttgaggttc cagggaaaat
     4861 agccctggtc aaaattctgc ctcccggcga aattccacat aaaatacctc cagtacatcc
     4921 acatcacctg gtaccggatg aagaagctga cattgtcggc ctgctcgggt ttggcttttc
     4981 cattcatcca gtattggtaa aggctggcac gatcggaatg tcccatccgg gggaaaaatt
     5041 tctggtctga ggaccggtaa atcggggtga attttttatc caccacttca tagcggtcgc
     5101 caacccttcc ataccggtct tcactggtat agtcgatggc agaggcatta taagccgggc
     5161 cccggagcag ggggcggtcg ccatattgtt cgcggttgag gtaggggatc aggcgcatgg
     5221 cgtccgaggg atcattcata ttgatcggag ggttcgcgtt ggcgcggatc acgacgacgg
     5281 ctaccgtgga gaagcccagg ataaccagga gggaggtgag cacaatgttt tgcaacatgg
     5341 cgcttccgtt cttgcgggcc aggtgaaggc cataatatat accggccccg atgaccagga
     5401 taaaaggaaa gataccggta tggaagggca tgcccagccc attgaccatc atataatcaa
     5461 aaaggctcca gagtttgggt aatcccacga tcaccagttt ctggtacaga cccagcacga
     5521 cgatggatcc cgccagggca aaaaatgcgc cccggaggtc gtgtttctga aaccgcttgt
     5581 aataataaag caagccgatc acggggaagg tcagcaaact cagcaaatgc acacctatgg
     5641 ataaagcggc tacaaataaa gccaggatca gccatttttc attttgggcg ctggagggca
     5701 ggtaatacca cttgagtcca gcccataccg tgagggcggt gaaaaaagtg gacatcgcat
     5761 acacctcccc ttctaccgca ctgaaccaaa ccgaagtgca aaaagctccg gtaaggccgg
     5821 caaccagtcc ggcggcggac aagaccagtc cctggtgtgc atccggttcc tgatccttac
     5881 ccagccaggc cagtttggac aacatgatgg tggaccagca gacgaaagcg acggcgaaag
     5941 cggtgcaaat acctgaaagc aggttaatgg cgaaggcctg ggcagagggc tctgaactga
     6001 tcagggatgc cacccaggca aacaggcggc cgaccaggat aaaaagaggg gctcccggcg
     6061 ggtgcacgac ctggagtttg gccgctccaa gaataaattc accgcaatcc cacaaactgc
     6121 ccatgcgctc ggcagtcaga taatatacca ggaaggccag gccgaatacg agccaacctg
     6181 caatattgga ttgctttcga aatgtatcca taatttcaga tgatgaaaat caattaattc
     6241 gctcagaacg gcgcaaagct aataaatttg caggtctgga ggaattgcat taagaaaact
     6301 ttatatgctt aaactactct tacttgcggc cattggttat atcgtttacc ggtacgtctg
     6361 gcgtgatcct tccatcgata aagcccagcc ccgcaaaccc ctccgggatt ctccctttgc
     6421 gaacgacgag atcgaggata ccgaatacac agagaaaaaa tgaaccccac cggcctcaaa
     6481 gacggaatca ttcaatccaa ccatcatttc atcgtttaca ataaaccggc cggcatggcc
     6541 gtacagtctg tccaccacaa ggaagagtcc ctggaatcca aactcgccca ttattgcaaa
     6601 acaaaacttt tcctgattca ccggatcgat cagccctgca gcggactggt tctcttcacc
     6661 aaaaagaaaa ccggggcagg actcatcaat gaacaactta aaaaccagga agtcgagagg
     6721 atctacctgg cggtggtcca gaccaaaccc gaaccggaag ccggcaccct gcatcacttt
     6781 ttattcgcac ataaaaaaag caacaaatcc ttcgtggtgg atgaaggaca caaggaaggg
     6841 aaagccgccc aactccatta tcaatggctg ggcacctccg gcggcaacca tctgctgatg
     6901 atccggttga aaagcggaag gcatcaccag atccgggccc aacttgcggc catcggcagc
     6961 ccgatcaagg gcgatgtcaa gtacggcgca aaaaaataca acgaggacgg ccggatccat
     7021 cttcatgcct ggaagctgaa gttttcgcat cctgccaatg gttccccggt cagcttcgaa
     7081 gcccccattc cggaagaagc cacctggcaa tccctcacag cctttgtccc ctcctgattt
     7141 taattttaat caaaccctcc ccgaatatgg aagaaaaaaa gaccgatgtc aatacgctgg
     7201 gcgaatttgg actgatcgac cacctcacgc gcaataccgt ccgttatcac cgggaaacgc
     7261 tttgcggagt cggtgatgat gcggcggttc tggaaggttc cgaccagctc accctggtca
     7321 ccacagacct tttgatcgaa gggatccact ttgacctgat gtacaccccg ctcaaacacc
     7381 tgggatataa aagtgtggtg gtcaatgttt cagacctctg tgccatgaat ggaaaggccc
     7441 ggcagatcac caccagcatc gccctttcca accgcttttc cgtggaagcc ctcgaccaga
     7501 tctatgaagg cattcatctg gcctgtaaac attatgaagt ggacctcata ggaggagata
     7561 ccagttcttc acccaagggc ctgtacctaa gcagcacggc catcggccag gtcgcacccg
     7621 atcaggtcgt ctaccgttcc ggcgcccggc ccagggacct gatctgcgtc accggggatt
     7681 tgggttccgc ttacctgggg cttcaactgc tcgagaggga aaaacagatc tttcagagcg
     7741 cgcccggcat ccaaccggag ttgaacgaaa aatacagtcc cctcatcggc cgtcagctca
     7801 aaccggaagc ccgccgggac gtagtggaaa gttttcacaa acttcatgtg aaaccacatg
     7861 ccatgattga tatcagcgac ggtttaagct ccgaaatcat gcatatctgc aaacaatccg
     7921 gtacgggcgc cctcattgaa gaaaacctgg tgcccatcca ccccctggcc accgaactgg
     7981 cacttgaatt taatctcaac cccatcactt gtgcgctcaa cgggggcgag gactatgaat
     8041 tgttgtttac gatttccgag aaagaatttg aaaggatcaa atacctgccc ggtttttatg
     8101 tcatcgggga gatccggccg gcggaagaaa aaatcctgct ccggacctcc ggaggcaaat
     8161 acgttcccct cgaagcccag ggctggagac atgtatgacc agaatacact gtctttctaa
     8221 gagctgtcag ctatcagcag catggtccaa ctacagtttt aaaaaagttc tgatccttac
     8281 ctttgcacgg ccaggacaga ggtactaaag acccaggcta tgaaaataat ccgcatcgtg
     8341 ttttttacgg taatagctct taacccggcc gtcggccaga cctttttttt cggcaatgat
     8401 ctttcttacg tcaacgaaat ggaggactgc ggagtgattt acaaagaaaa taaaaaagcc
     8461 aaagacccct accggatctt cgctgatcgt gggtgcaacc tggtacggct ccgactctgg
     8521 aaagatccaa gctggtacaa aagcctgaac cagggcaaaa tttattcgga cctcggtgat
     8581 gtgatgaaaa gcatcggccg tgccaatgcg aatcaaatgc aggtattgct ggattttcac
     8641 ctgtccgata attgggccga cccgtccaaa caactcgtac ctgctgcctg ggaacccgtg
     8701 gtgaataatc ttccggtttt aaaagacagt ttgtaccggt atatctataa cacccttcag
     8761 attttattcc aggcggacct attaccggaa atggtgcaga tcggcaatga aaccaataaa
     8821 ggcatcctcc tttccccggc ggataatgcg aaatacaccc tcgactggcc gcggaatgct
     8881 gaacttttta atgccgcgat cgcggcggtc cgggattttg aaaaaagcag caagcaaaaa
     8941 atcaaggtgg ccattcatgc cgccgggccc aaagactccg gctggctcat cgaccagttt
     9001 gttcagaatg gggtgacgga ttttgacctc atcggcatat cctattactg ggcctggcac
     9061 aaacccagtt ccatcgaaga aacgggggcc ctcatccaat ccctcaaaaa caaataccgg
     9121 aaggaagtga tgatctttga aaccgggtac ctctggacta ccgcgagcca ggaccaggcc
     9181 aataatatta tatccgaagt ccatcccgac tatgcaccgg cgagtccgga aaatcagaaa
     9241 aagtggctga tcgaccttac gaaggaagtg atgcttaagg gcggcagcgg ggtcatctat
     9301 tgggaaccgg cctgggtatc ctccccctgc ttcacccaat ggggccaggg atcccaccag
     9361 gaacacgcgg ctttttttga ctttgagcat gatttgatcg aagaaggggg gatcggctgg
     9421 atgtcttacg actacctttc gaccgtaccc ctcgaatccg accataccct aaacggcatc
     9481 cgggtggtcg tggacagcct tgcaaaaaaa ctgcaaatcg aaacggccgg ccccgaaaca
     9541 tccatacgcc ggatcaggat ctatgatatg aaaggaagcc tggtcctggc ggaatctccc
     9601 ggggaagcag gcagggtata tactttatca ttacaaagcc tgggcagcag cctctatatt
     9661 ctggaagtct gggaccaatc caacaaatcg ttcagacaga aaataatgct ttgaaataca
     9721 gttttgtttc atttttgtat aaataagtac taatatttta tccggacagg ttaaaatcgt
     9781 tttatctttt tttctacatt tggtcacgat tttttttaac actcgttctc ctattgtatg
     9841 aaaaagttac tgctgcttgc cctcaccctc tttggcttta cccttgccga cgctcaaagg
     9901 accgttacag gtaccgtttt tgacaattcc accaacgaac cgctgattgg agcttccgtc
     9961 caggtcgaag gctccaacca gggagtgatt acggatatcg atggcagcta ttccatccag
    10021 gtcccggacg gggcggtccg gctaattgta tcttatgtag ggttcactac tcgcatcgtg
    10081 gaggtgggca atgccagtac cctggacatt gtccttgaac cgggacagca actgcaggaa
    10141 atcgtggtga tcggatatgg caccgtcaaa cgggaagatg ccacgggatc catccaggtc
    10201 gtgaccagtg aaaaatttaa taagggggcc attaccgcgg ccccggaact cttgaccggc
    10261 aaaatcgcgg gcctccaggt gaacaacagc agtgaacccg ggggagggtc ggtgatccgg
    10321 atccggggcg gttcatccct cagcgcatcc aatgatccgc tgatcgtcat tgacggcgtg
    10381 cccgtggcca acgacggcat ttcgggcgac cgcaatgtat tgaatattgt caaccccaac
    10441 gacatcgaat ccgtcaccgt cctcaaggat gcttcggcta ccgccatcta tggttccagg
    10501 gcctccaatg gagtgatcct gatcaccacc aaaaaatcca gaacaactca gaaaatcgcc
    10561 gtagactaca gtggaagtta tgccctgagc aacaggatca atgaagtcga ggtgcttagc
    10621 gcggacgaat tcaggactta tgtgcggacc cgttatcccg attcacatcc cgcgccggct
    10681 ttgctgggca atgccaatac cgactggcag gacctgatct ttcagaaagg tcagaccctg
    10741 gaccagaatc tcagcttgtc cgggcgggta ggcatcctgc cctaccggtt ttcggtgggt
    10801 tataccgacc gtaccggcct gttaaaaaca gataagttca accggaaaac cgcggctttg
    10861 aaactcacgc ccaaattcat caacaatacc ctccaggtga tttttgacgt aaaaggaatg
    10921 ctgatcaaca accgtttcgc cgactgggga gccatcggat cagccac
//
LOCUS       DAOQXT010000133        10954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6424_length_10954_cov_7.202496, whole genome shotgun sequence.
ACCESSION   DAOQXT010000133 DAOQXT010000000
VERSION     DAOQXT010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6424_length_10954_cov_7.202496"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..2037)
                     /locus_tag="PKM27_17185"
     CDS             complement(19..2037)
                     /locus_tag="PKM27_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR09058.1"
                     /translation="MFIPFGRLVIVFSALAWTLASRAQDQVDTLSSLSANLPVIEINA
                     YSAKSDLVRIPAAVQLIRDTLHLFPQLSFAQAINQAPGVKLEERSPGSYRLSIRGSTL
                     RSPFGIRNVKVYLDGFNLSDGGGNTYLNLFEPGLVSKSEILKGPASSLYGAGTGGVLL
                     LSTDPTQNQLSLSYGTYDQFTESISLYKNFARWKVSVFQSHQQSEGYREQSAMRRDLL
                     YASQRYKSGRHNLIFVQAYGDLKYETPGGLTFDQQKSNPRQARPKTATLPGAVEQEAS
                     IFNKTLWTGAAYDYQINRSWSLNPNFTAWYTDFRNPFITNYEIRFEKNFSARPVLKYF
                     TTTPGGSFEWTNGLEYLFQGGRVKNYVNNKGEKGNLTGAADIDGIQNNWFSQINWNLK
                     RWQWLAGMSLNQQVYRYKTPGLEDDQRKESGPVFMPRASVSYSLGREDAVFISAARGY
                     SPPSLAEIRPSSGEYNQELLPEQGWNFELGIKGYRNRLQYSFNLYSLNLRDAIVRRND
                     AAGVEYFINAGGANIRGLEAWLQYNFSWLELYGSTAYQPYEFVDYKQRTDDFSGNRVT
                     GVPRAVHTFRLSLNPFAAMLLGGGWYYQSDMPLQDANTSFHPAYSLVNLFANYRLPIS
                     GQNISLSVAVDNLFNTAYSPGPDINAAFNRFYNPGLLRTVVVGLKWKM"
     gene            2208..4154
                     /locus_tag="PKM27_17190"
     CDS             2208..4154
                     /locus_tag="PKM27_17190"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HNR09059.1"
                     /translation="MINGQSRVFAAVLSPVVDGGKYSIRRVVGQWVHVDARIICDGHD
                     VIQAALQFKPAGSRTWQEHRIWGKAEDLWGGSFRVEAQGLWDYRVCGWVDHGLTWREG
                     LSKKANDGQFVKTELQDGLHVLKAILDMKKQSEEVVDFLTRLIDLIGDENQYQEALQM
                     ALSNDLQHILYTCPEKKFISYSETYQVYVDRPRALFSAWYEFFPRSASREPGKHGTFK
                     DCEQLLPRIKEFGFDVLYFPPIHPIGEVNRKGKNNSTVAAEGDVGSPWGIGSRNGGHD
                     AIHPELGTLADYKALIQKAREMDIEIAMDFALQCAPDHPWVKEHPAWFKWRSDGTVQY
                     AENPPKKYQDILPLYFETEDWRALWTAIRQVVLYWIEQGVTIFRVDNPHTKPFAFWEW
                     LIAEVKKQHPDVLFLSEAFTRSALMYHLGKIGFTQSYTYFSWKNSKYELIDYMNELTK
                     GSTGEVFRPNFWPNTPDINTFPMQNGNQTLYMIRIFLAATLSSSYGLYGPAYELLVHE
                     ALPGREEYYRSEKYEIYHWDWEARNALTMLITMLNKIRNAHPAFHETNNWENVNVQND
                     HLIAYYKQNADGSDKVLCVVNLNPYERQSASLQAPLHRIGVYPGQQFTVHDLISGNSW
                     IWNQEWNYVALDPGLPFHLFHIRQ"
     gene            complement(4221..6335)
                     /locus_tag="PKM27_17195"
     CDS             complement(4221..6335)
                     /locus_tag="PKM27_17195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR09060.1"
                     /translation="MALISKIRKHSWIIVASLALALLSFLIMDVVSNNTFGGQTQFTV
                     GKVNGVAIDYNDFSRTEQILYNNSAVDPYSRRTYLWNYFVEKILMEKTASKLGIGISP
                     TELEELQFGNNLSPVIQQRFLDPSTNMVDRDQLNQIKQGLESNTLRADLVTFWNEQKT
                     EIVKERLQAKYAGLVSKAIYSPTWMAEQRHFDATELVDFQYVKLPFDLIMDSISVTDQ
                     EIEKYIKDHKVGYTYPEEARFLEYVSFPVIPTAEDSAAIRTELNVLVDSFKNTREDSL
                     FVALNGGQLDPAYFRKDQLSLEFAESLFDLPKGGTYGPYIDNGVYRLAKMVNRKIIPD
                     SVDVRHILRSVQTIDQMVSAQKTIDSLKNLLESGAARFDSLAIRYSQDPGSGFNGGEL
                     GMTGHDVMVKPFNDVIFFQAEPGKYYSVTTQFGIHLIEVKKQVFGKNPAESVQLAYLD
                     RPIVPSQATQDRVEDEVTAINNANRTLDELKKALASRPELVFEKTGAVKKNDNTAGLL
                     PAGESTRSMIRWAFDKKASPGDVSPEIFLYEDAVNFVTNQINIAGLRSIQPKGLARVE
                     AVREDVTLTLTNKKKAERLISEIQGTPDIYALATKYNVKVDTATRVNFTTSFLQQMGN
                     EPKVIAAAFGTEQNATSKPIIGNGGVYVVKTTGRSPSIGEPDIAFFREQVSSVSRNNV
                     TSFLMESLKKRAKIKDNRFTFF"
     gene            complement(6454..8298)
                     /locus_tag="PKM27_17200"
     CDS             complement(6454..8298)
                     /locus_tag="PKM27_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR09061.1"
                     /translation="MSIRPGPPPEFDNQTRKGFWESLKALKLLPPFFRMIWRTSPSLT
                     LANILLRLVKSAVPLGQLWVGKLIIDQVIYCINHKGEGLDSLWTYVGIEFGLAVFSEI
                     FNRLISLTDSLLGDLYGNTSSVELMNKAATIDLSMYENPDFYDKLDRARRQTTGRVVL
                     MSMVLSQLQDLITIGFLSAGLIAFEPWLIVLLVVAVLPSFLSEAYFSRSSYSLVRSWT
                     PQRRELDYLRYIGASVETAKEVKVFGLDRFLADRFGKLAAEYYKLNKSLAIRRTLWGT
                     LLQIGGVLAYYGAYVLIVLRTISGALTIGDLTFLSGSFNRLQNQLQSLLSNFTRITES
                     ALYLQDYFDFLALEPQIRNLPDARPAPGKVTRGIRFENVGFKYPGTETWALRHLNFEI
                     RAGEKMALVGENGSGKTTLVKLVARMYDPSEGAIYLDGVDIRNFELESYRKMISVIFQ
                     DYVRFSFKAGENIAVGKIEDVRDLEQVMSAATKSLADAVIAKLPAGYDQMLGKRFAEG
                     LDLSGGEWQKIALARAYMRDADIVILDEPTSSLDARAEYEVFKRFSELAAGKTSIIIS
                     HRFSTVRMADRILILKNGEMLELGTHQELLDRKGLYHELFTLQAKGYQ"
     gene            complement(9036..9311)
                     /locus_tag="PKM27_17205"
     CDS             complement(9036..9311)
                     /locus_tag="PKM27_17205"
                     /inference="COORDINATES: protein motif:HMM:NF022840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR09062.1"
                     /translation="MEMKVAEITIKNIRGIKNANILLPRHGVLIGDNNIGKTTVLEAL
                     DLVLGPDRLNQQPPIDEHDFFKAEYTAVNATLMISSTIDETTDTVQN"
     gene            complement(10010..>10954)
                     /locus_tag="PKM27_17210"
     CDS             complement(10010..>10954)
                     /locus_tag="PKM27_17210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNR09063.1"
                     /translation="FLQENHYYPFGMEQGGWTVPSSPENKYKYNGKELNDDFSLGLYD
                     YGARWYDPVVGRWTQIDPLAEMMPGWSPFNYGFNNPIRFVDPTGMAPDDWVKGADGTI
                     RWDASANSQATTKQGETYLGKELTLTFNSFIDAKSWDGPNSKAPGDKLTSTITITGNE
                     DSKGELTSLSGSKSVQIGSTPIGTARDYYPGEGGSNNNFELKSTASGATLNFEQHASV
                     SKIEEIGLNALGFKVVDVAQKLNVDYNKKSGELSISAYTGLFPSATLSINGTQVMKYN
                     QPSFVNTHSAPIVGNSSPSSGSIPIRNFSYYPSKLYKQ"
BASE COUNT         2819 a         2644 c         2635 g         2856 t
ORIGIN      
        1 aacttgctaa cttactaact acatcttcca cttcaagcca accaccaccg tccttaacaa
       61 gcccgggttg taaaaccggt taaaggcggc attgatgtcg ggaccgggac tatacgcagt
      121 attgaataga ttatcgacgg ctacggacag gctgatgttt tgtcctgaaa tgggcaacct
      181 gtaattggca aacaggttga ccagggaata agcaggatga aaactggtat tggcatcctg
      241 gaggggcata tcggattggt aataccatcc gccgccgagg agcattgcgg caaagggatt
      301 aaggcttaat ctgaaggtgt gcacggcgcg gggcacgccc gtcacccggt tgccgctgaa
      361 atcatcggtc ctttgtttat aatccacaaa ttcataaggc tggtaggcgg tcgatccata
      421 gagttccagc caggagaaat tatactgaag ccaggcttcc aggccccgga tattggcgcc
      481 accggcattg atgaaatact ccaccccggc ggcatcgttg cggcggacga tggcatcccg
      541 taagttgagg ctgtataggt tgaaactgta ttgtaagcgg ttgcggtacc cttttatgcc
      601 cagttcgaaa ttccagcctt gttcaggcag caactcctgg ttgtattccc cggaagaagg
      661 cctgatttct gccagggagg gaggcgaata accgcgcgct gcagagataa atacggcatc
      721 ttcccggcca aggctatagg aaacggaagc cctgggcatg aacacggggc cgctttcttt
      781 ccgctgatca tcttcgagcc cgggcgtctt ataccggtac acctgttggt tgaggctcat
      841 tccggcgagc cattgccacc gtttcaggtt ccagtttatt tgagaaaacc aattattctg
      901 aattccgtca atgtccgccg caccggtgag atttcccttt tcgcctttgt tgttcacata
      961 attttttacc cgtccgccct ggaatagata ctcaagccca ttggtccatt caaaactgcc
     1021 gccgggggtg gtggtgaaat atttcagcac cgggcgtgcc gaaaaatttt tttcaaaacg
     1081 gatttcataa ttggtgatga aagggttcct gaaatcggta taccaggccg taaaattggg
     1141 attgaggctc caggaccggt tgatctggta atcgtaagcg gcacccgtcc agagggtctt
     1201 attaaaaatg ctggcttcct gttccacggc tccgggcagg gtggctgttt tgggcctggc
     1261 ctgccgggga ttggattttt gctggtcaaa ggtcaggccc ccgggggttt cgtatttcag
     1321 gtcaccataa gcctgcacaa aaatcaggtt gtgcctcccg cttttgtagc gctggctggc
     1381 atacagcagg tcccttcgca tagccgattg ttcgcggtag ccttcacttt gctgatgcga
     1441 ctggaagacc gataccttcc acctggcgaa gtttttatac agggatatgc tctcggtgaa
     1501 ttgatcgtaa gttccatacg acagggacaa ttgattctgc gtcggatccg tggataacaa
     1561 caggacccct ccggtgccgg caccgtataa gctggaggct ggacctttga ggatttcgga
     1621 tttactgacc aggccgggtt caaaaaggtt gaggtaggtg tttccacccc cgtccgaaag
     1681 attaaatcca tcgagataga ctttgacgtt ccggatccca aagggcgatc gaagggtgga
     1741 tccgcggatg cttaatcgat aactgcccgg ggatcgttct tccaatttta cccccggggc
     1801 ctgattaata gcctgggcga aagaaagttg gggaaaaaga tgcagggtat cccggatcag
     1861 ctggactgcc gcggggatgc gcaccaggtc gcttttggcg gagtacgcat tgatttcgat
     1921 cacaggcagg tttgctgaca aagaagacaa ggtatccacc tggtcctgtg cccgggaggc
     1981 cagggtccag gccagtgcag aaaatacgat aaccagcctg ccaaatggta taaacataaa
     2041 agatccgggc ttacatgaat gggaaacaaa aatacagtcc ggaaaggata tgtggaggcg
     2101 ggtgcgagca tggaggccgt tcacacctga cccaccgctc atggctcaaa gttccttccc
     2161 gtatttctcc ctaaattcgc ccacaaatct atcaaaccag aaaatctatg atcaacggtc
     2221 agagcagggt atttgcagcg gttttgtccc cggtcgtgga cggcgggaaa tacagcattc
     2281 gcagagtagt gggccagtgg gtccacgtgg acgcaaggat catttgtgat ggacacgatg
     2341 tgattcaggc tgctcttcag tttaaacctg ccgggagcag aacctggcag gaacaccgca
     2401 tctggggcaa ggcggaggat ctctgggggg gtagtttccg ggtggaggcc cagggccttt
     2461 gggattaccg ggtttgcggc tgggtggacc atgggctgac ctggagggaa ggcctttcca
     2521 aaaaggcaaa cgacggccag tttgtaaaaa cagaacttca ggacggcctg catgttttga
     2581 aagccatcct ggacatgaaa aaacaatccg aggaggtggt ggactttctg acccgcctga
     2641 tcgacctgat cggggatgag aaccaatacc aggaagccct ccagatggcc ctctccaatg
     2701 atcttcagca tatcctttac acttgcccgg agaaaaaatt tatttcatac agtgagacct
     2761 accaggttta cgtcgaccgg ccccgggccc tattcagcgc ctggtatgaa ttttttcccc
     2821 gttccgcctc ccgggaaccg ggaaaacacg ggacattcaa agactgtgaa caactcctgc
     2881 ccaggatcaa ggaattcggt tttgacgtgc tttattttcc gcctattcac cccatcgggg
     2941 aagtcaaccg gaaaggaaaa aacaattcaa cggtcgcggc agaaggcgac gtaggatctc
     3001 cctggggcat aggcagccgg aatggaggcc acgatgccat ccatccggaa ctgggaacgc
     3061 ttgcggatta caaggccctg atccaaaaag cccgggaaat ggatatagag atcgcgatgg
     3121 attttgccct tcaatgcgca ccggaccatc cctgggtcaa ggaacatccg gcctggttca
     3181 aatggcgcag cgacggtacc gtccaatatg ccgaaaaccc cccaaaaaaa taccaggaca
     3241 tcctgccctt gtattttgaa accgaagact ggcgggccct gtggacggcc atccgccagg
     3301 tcgtactgta ctggatcgaa caaggggtga ccattttcag ggtggacaac ccccatacca
     3361 aacctttcgc cttctgggaa tggctgattg ccgaagtgaa aaaacagcat ccggatgtac
     3421 tctttctctc cgaagctttt acccgttccg ccctgatgta ccatctgggc aaaatcgggt
     3481 ttacccaaag ctacacctac ttctcctgga aaaattccaa gtacgagctg atcgattata
     3541 tgaatgaact gaccaaaggg agcaccgggg aggtgttcag gcctaatttc tggccgaata
     3601 cccccgacat caatactttc ccgatgcaga atggcaacca gaccctgtat atgatccgca
     3661 tcttcctggc ggccaccctg agcagcagtt atggcttgta cggtcctgcc tatgaattat
     3721 tggtccacga agcccttccc ggccgcgaag aatattaccg gtcagaaaaa tacgagatct
     3781 accactggga ctgggaggcc cggaacgccc tcactatgct gatcaccatg ctcaataaaa
     3841 tcaggaacgc ccatcccgcc tttcacgaaa ccaacaactg ggaaaatgta aacgtacaaa
     3901 acgatcacct gatcgcttat tacaaacaaa acgcggatgg aagcgacaag gtcctttgtg
     3961 tggtcaacct gaatccttac gaaagacaat cagccagcct gcaggctccg ctgcaccgca
     4021 ttggcgttta ccccggccag cagtttaccg tccatgacct gatcagcggc aacagctgga
     4081 tctggaacca ggaatggaat tatgtagctt tggatccggg attgccgttc catttgtttc
     4141 atatacggca gtgatggtgg gtggtgagta gtggttggtg aatgcagaac ctctcgcttc
     4201 tcgcttctcg cttctcgctt ctaaaagaac gtaaaccggt tatccttgat ctttgccctc
     4261 tttttaaggg attccatcag gaaggaagtc acattgttcc tggaaacaga agaaacctgc
     4321 tcacggaaaa aagcaatatc gggttcgccg atggagggcg atctgccggt cgttttgacc
     4381 acataaactc ctccgtttcc tatgatgggt ttggaggtcg cgttttgttc ggtaccgaaa
     4441 gcggcggcta tgactttggg ttcattgccc atttgctgaa ggaaactcgt ggtgaaattc
     4501 acgcgggtcg ccgtatccac cttaacattg tacttggtgg ccagggcata aatgtccggg
     4561 gtgccctgga tttcactgat caggcgttcg gcttttttct tattggtcag ggtaagggtg
     4621 acatcttcac gcacagcctc cacccgggca aggcccttgg gctgaatgga ccggaggccg
     4681 gcgatgttaa tctggttggt gacaaaattg accgcatctt cataaaggaa aatctcgggc
     4741 gacacatccc cggggcttgc ttttttgtcg aaagcccaac ggatcataga gcgggtgctt
     4801 tccccggcgg gcaggaggcc ggcagtattg tcgtttttct ttacggcccc ggttttttca
     4861 aaaaccaatt caggccttga agcaagcgct ttcttcaatt cgtccagggt gcgattggca
     4921 ttgttgatgg ccgtcacctc atcttccacc cggtcctggg tggcctgcga aggcacaatg
     4981 ggacggtcga gataggccag ttgtacgctt tccgccggat ttttgccgaa tacttgtttt
     5041 ttaacttcga tgaggtggat gccaaactgg gtggtcaccg agtaatattt acccggttcc
     5101 gcctggaaga aaatgacgtc gttgaacggt ttgaccatga catcatggcc cgtcataccc
     5161 agctcgcctc cgttaaatcc cgaacccggg tcctgggaat accggatcgc cagcgaatcg
     5221 aaacgggcgg ctcccgattc cagtaaattt ttcaggctgt cgatggtttt ctgggcggaa
     5281 accatttgat caatggtctg gaccgatctc agaatatgtc gcacatcgac ggaatcggga
     5341 attattttgc ggttgaccat tttggccagc cggtacaccc cattgtcaat ataaggtcca
     5401 tacgttccac ctttgggcaa gtcaaacaag gactcggcaa attcgaggct gagttggtcc
     5461 ttcctgaaat aagcagggtc cagttgtcct ccgttgagtg ccacaaacag ggaatcctcc
     5521 ctggtatttt taaaactgtc caccaaaaca ttcagctcag tccgaatggc ggcgctgtct
     5581 tcggcggttg ggatgaccgg gaagctgaca tattcaagaa aacgggcttc ctcgggatag
     5641 gtataaccga ctttatggtc cttgatgtat ttttcgattt cctggtccgt gacgctgatg
     5701 ctgtccatga tcagatcaaa aggtagtttg acatattgga aatccacaag ctccgtcgcg
     5761 tcaaaatgcc tttgctctgc catccaggta ggtgaataaa tggctttgga gaccagcccg
     5821 gcgtatttgg cctgcaggcg ttccttgacg atctcggttt tctgctcgtt ccaaaaggtc
     5881 accaggtccg cacgcagcgt gttgctttcc agtccctgct tgatctggtt gagttggtcc
     5941 cggtccacca tgttcgtgct gggatccaga aaacgttgct ggatgacggg gctcaggttg
     6001 ttcccaaact gaagttcctc cagttcagtg ggactgattc cgattcccag tttggaagcg
     6061 gttttttcca tgaggatttt ttccacaaaa taattccaga ggtaggttct cctggaataa
     6121 ggatccaccg ccgagttatt gtaaaggatc tgttccgtcc ttgaaaagtc attgtagtcg
     6181 atggccaccc cgttgacctt gcccaccgta aactgggttt gtcccccgaa ggtattgttt
     6241 gacacgacgt ccatgatcag aaagctcaat aatgccaaag caagggaagc cacgataatc
     6301 caactgtgtt tgcggatttt actgattaaa gccattttaa atcaataatt tataatataa
     6361 taaaatattt aatacgagtg gtcgccggac cgcttgtaaa aaagcgaatg caaaagtaag
     6421 atttttgcat cattcccggc cttttttaaa agctcactgg taacccttgg cctggagagt
     6481 aaacagctcg tggtacagcc ctttccgatc aaggagttcc tggtgggtgc ccagttccag
     6541 catttccccg tttttgagga tgaggatccg gtcggccatg cgcacggtgc tgaaccggtg
     6601 ggagatgatg attgaggtct ttcctgccgc cagttcggag aaacgtttga acacttcata
     6661 ttccgcccgg gcgtccaggg aggaggtggg ttcatccagg atcacgatat cggcatcccg
     6721 catataggcg cgggcgaggg cgattttctg ccattcgcct ccgctgaggt ccaggccttc
     6781 ggcaaatctt tttcccagca tttggtcata accggcggga agtttggcaa tcaccgcatc
     6841 ggccagactt ttggtcgccg cggacatcac ctgttccagg tcccggacgt cttcgatttt
     6901 acccaccgca atgttttcac cggctttaaa actgaatctc acatagtcct ggaagatcac
     6961 actgatcatt ttgcgatagg attccagttc aaaattccgg atatccactc catccaggta
     7021 aatggcccct tcggaaggat catacattct ggctacgagt tttaccaggg ttgttttgcc
     7081 gctgccattt tcaccgacca gggccatttt ttccccggcc cggatttcaa aattcaggtg
     7141 ccgcaacgcc caggtttctg tgccagggta tttaaacccc acgttttcaa accggattcc
     7201 ccgggtcacc ttgcccggag caggccgggc atccggcaaa ttccggatct ggggttccag
     7261 agccaggaaa tcaaaataat cctgcagata taaggcggat tccgtgatac gggtaaaatt
     7321 ggataacagg ctttgaagct ggttttgaag gcggttgaac gaaccggata aaaaagtgag
     7381 gtccccgatg gtcaatgcgc ctgagatggt gcgcaggacg atgagcacat aggccccata
     7441 ataagccagc accccgccga tctgaaggag ggtcccccaa agcgtcctcc gtatggccag
     7501 ggatttgttc aacttatagt attcggcagc cagtttgcca aaacggtcgg ccaggaaccg
     7561 gtccagtccg aaaaccttca cttctttcgc cgtttccacg ctggctccga tatacctcag
     7621 gtagtccagt tctctccgct gcggggtcca gcttctcacc agggagtaac tggaacggga
     7681 aaaataagct tcgctcagga aggaaggcag taccgcgacc accagcaaaa caattagcca
     7741 gggttcaaaa gcgatgagcc ccgcgcttaa aaaaccgatc gtgatgaggt cctgcaactg
     7801 gctcagcacc atgctcatca aaacaacgcg gcccgtggtc tggcgtctcg cccggtccag
     7861 cttgtcgtaa aaatccgggt tttcatacat gctcaggtca atcgtggccg ccttattcat
     7921 caactcgacc gaactggtat tcccataaag gtcgcccagc agggaatcgg taaggctgat
     7981 cagccggttg aatatttcag aaaaaacggc caggccaaat tcaatgccga cataggtcca
     8041 cagggagtcc aagccttccc ccttgtgatt gatgcaataa atcacctggt caatgatcag
     8101 ttttcccacc cagagctggc ccagcggaac ggctgatttg accagtcgga gcaggatatt
     8161 ggcaagggtg agggagggcg aagtcctcca gatcatccgg aagaaaggag gcagcagttt
     8221 gagcgctttc aggctttccc agaacccttt gcgggtctgg ttgtcaaatt cggggggtgg
     8281 gcctggtcgg atggacatgg gggaaggttt gaggtgcgag attagaggga cgaggtacga
     8341 agttaggggt taatggtttt tcagctctaa caatgtccca tttcaggaca tcctcaccct
     8401 gccagggata gataggtatt actatcgggt aatgcatacc aaaaggaaaa cctcgccttg
     8461 ttttgaggct ctcgaatgcg tcgaaaaata aataggcatt ggcaggccta cacaatacaa
     8521 ttgaatcgac atcggtacaa aacataacct cagtcgaccc tcaccgctac aaagttctct
     8581 gacgagaaca attgatttct taacatagta aactatagtc tgactgatag ggggctgaac
     8641 cacactttca aacgcaatat aatatctaat aaactacctc atttcaggct tagggtcctc
     8701 aagccggtag gcagagatcg cagacacaga aaaccacaaa cttgtttatc ttttttcgaa
     8761 aatagttcgg gcttgtcgtc ctcagtgagg ctccggcaga agtatgttct tccctcaaag
     8821 ccatcctctg ctggatcgta ccagccgtga aatgttattc gtattgcttc cttgacattg
     8881 ctttggtgac acctgctgag ttggcttcgg tgtaaagcgt attcgatgtt gaatcccaaa
     8941 atttaattta atcgccaaat tttgccttct gttcttctgt taaatacgca atcgttacct
     9001 caatctcgat tcttgaagcc cttgcaacta tatcatcaat tttgaacagt atctgtggtt
     9061 tcatcaatag tactgctaat catcagtgta gcattcacgg cagtatattc tgctttgaaa
     9121 aaatcatgct catcgatggg tggttgttga ttgaggcggt caggaccaag aacaagatcg
     9181 agtgcctcta aaacagtggt ttttccaata ttgttgtcgc caatcagcac accatgcctg
     9241 ggtaatagaa tatttgcatt ctttattcca cgaatattct ttattgttat ctcagcaact
     9301 ttcatttcca ttccctaagt tgataagatt tattttacac ttacatttta caatgttata
     9361 caaatgagca tcacttcaat ttttgcttta aaaatattct catccaaaat taattattag
     9421 agcttgtatg tttgtctgaa aatattgcaa tttgcattga aatttaattt ttgctaaatt
     9481 acaaactcca aattaaaata ttcttggatt acatttattt atgagagaca aacgattaaa
     9541 catatttcaa gcttttaaag tgatgaagga attgttgtat aaattatttt tataaaaatc
     9601 cgaatttaat cattcttaaa tagccttaac cttagatatt tataataaac aaccacaaac
     9661 acaataagcc ctatacccaa attgtatata ccatttctcc agtctgattc ggaggcaagg
     9721 aatgaatata caaagtattc taaaataaaa actacactta aaagtattgg aaagcctttt
     9781 aaattattgt gtttaaataa ataatgtatt ggaatcgtaa ataaaacaat acttattgcc
     9841 ggaagtgata aaaatatcca taaataatac atcagatcag aaccatttct tattccaggt
     9901 tgaactacat aaaaatcctg gttctttatc attaagaata tatagaatat taagtatttc
     9961 agaataaaat aaatacttat tccggaaatt gaaaatttgt tcacccgaac tattgtttgt
    10021 ataatttaga aggatagtat gaaaaatttc gtattggaat actcccagaa gatggtgaag
    10081 aattcccaac aattggtgca gaatgtgtat ttacaaatga tggttgatta tatttcataa
    10141 cttgtgttcc gttgatactt aaagtggctg aagggaacaa tccagtgtat gctgaaatcg
    10201 aaagttcacc cgatttttta ttataatcaa catttaattt ttgagcgaca tcaacaactt
    10261 taaagcctaa tgcattcaat ccaatttcct caatctttga tacactggca tgttgttcaa
    10321 agtttaaagt ggctcctgat gctgtggatt ttaactcaaa attattattg ctaccacctt
    10381 caccaggata gtaatctctt gcagtgccaa ttggggttga accaatttga actgactttg
    10441 aaccacttaa gctagttaac tcacccttag aatcttcatt tccggtaatg gtaattgtag
    10501 aagttagttt atcaccaggg gctttactat taggtccatc ccagctttta gcatcaataa
    10561 aactattaaa tgttaatgtt aattctttac caagatatgt ttcaccttgc ttagtagttg
    10621 cttgagagtt tgcacttgca tcccatctaa tagttccatc agcgccttta acccaatcat
    10681 ctggagccat gccagttgga tctacgaacc gaattggatt attgaaacca tagttaaaag
    10741 ggctccaccc tggcatcatc tcagccaacg ggtcaatctg agtccatctt cccaccactg
    10801 ggtcatacca tctcgctcca taatcataaa gccccaggct gaaatcatca ttcaattcct
    10861 tcccattgta tttatactta ttctccgggc tggacggtac tgtccatcct ccctgctcca
    10921 taccaaatgg ataataatgg ttctcctgca ggaa
//
LOCUS       DAOQXT010000134        10857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6505_length_10857_cov_5.102759, whole genome shotgun sequence.
ACCESSION   DAOQXT010000134 DAOQXT010000000
VERSION     DAOQXT010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6505_length_10857_cov_5.102759"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /gene="glnS"
                     /locus_tag="PKM27_17215"
     CDS             complement(<1..874)
                     /gene="glnS"
                     /locus_tag="PKM27_17215"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNR09064.1"
                     /translation="MSESLNFIEEIIERDLEEGKHGGRILTRFPPEPNGYLHIGHAKS
                     ICLNFGVADKYGGQTNLRFDDTNPETEEVEYVESIMADVRWLGFDWGDRQFFASDYFD
                     QLYEFALLLIRKGKAYVDHSSAEAIADQKGSPTQPGIESPYRIRSVEENLDLFSKMKE
                     GVFEEGECVLRAKIDMASPNMHMRDPVMYRIKKAHHHRTGDKWVIYPMYDFAHGQCDS
                     IEKITHSICTLEFENHRPLYEWYIDQLGIFPSRQYEFARLNLSYTVMSKRKLMQLVNE
                     QYVKGWDDPRMPTIR"
     gene            complement(884..2722)
                     /gene="argS"
                     /locus_tag="PKM27_17220"
     CDS             complement(884..2722)
                     /gene="argS"
                     /locus_tag="PKM27_17220"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNR09065.1"
                     /translation="MIKELLQNQLAQAIEELYPVEWPASELLVVPTKKDFKGDYTLTV
                     FPVAGKLRKNPMTVAQEIGNKMLENRLITSFEVVQGFLNMTLDDAIWTRTLQHIQGDP
                     KFGHGKPKNQTVLVEFSSPNTNKPLHLGHIRNMLLGSSTARMLEISGYEVRRVQVIND
                     RGIAICKSILAWSKWAGGATPSTTGIKGDHFVGDYYVLFEKEFAKEYLAWQSTPEAHQ
                     EFENRKDKELDEPAFFREFKNQYFNAYSLLGREAREMLLLWENHDPATLELWSRMNQW
                     VYDGFNATYDRMGVRFDHTDFESTTYLLGKEAVRKGLEMGIFYSKPDQSVWIDLSDAG
                     MDQKVVLRSDGTSVYITQDLGTAQMRYEKFKMNKLIYVVADEQDYHFKVLFEILKRFK
                     EPYADGLYHLSYGMVELPEGKMKSREGTVVDADDLVAEIIREAEKTAEERGDLGDFDR
                     AKQEEIIGKIALGAIKFFMLKVHPKKRMLFDPKESLDLQGQTGPYVQNAYVRIQSVLR
                     KSGERSEAGYQDYKGLAEEEKELISLMETYPQTVAKAAEAYDPSEVANFSYNLAKAYH
                     RFYHEVPILRADDPVALQFRLVLSDCVGRVLEDSFNLLGIDMPERM"
     gene            complement(2892..3446)
                     /locus_tag="PKM27_17225"
     CDS             complement(2892..3446)
                     /locus_tag="PKM27_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNR09066.1"
                     /translation="MRIIAGRLKGRRLNPPAKNWPTRPTTDFSREGLFNILENRLDFE
                     SLHALDLFGGSGSHSFELISRGCKEVTYVDLFRGCIDFVKKTSQDWKVDDCLTIVQDD
                     AIKFIGRAQKTFNYIFAGPPYALKELGRIPDMIFENRLLSPGGVFILEHNPNYNFTKH
                     PCFKEERKYGSTLFSFFEWPVEEG"
     gene            complement(3434..4108)
                     /locus_tag="PKM27_17230"
     CDS             complement(3434..4108)
                     /locus_tag="PKM27_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09067.1"
                     /translation="MSWIVFDDIFFILKEGINPLTLYSRADLFPSLEEHAAQGGMRII
                     LDTPFQLVPESLYDEKSGLNLLQAILPYPGYGESAVRTETWPSTYSKIIYYLPAPFGP
                     YHASETLHWMTCLAAYAQAWFDRHHTGLFTLRIQDTMYNLVQVNKVLKEASKLRLPSP
                     DDSTYFTLKLVEKYRSHQQAFKVWTNDPSTAHLRILKKFVPEVSLLQPPGPEMIKEII
                     QGACGS"
     gene            complement(4154..4741)
                     /locus_tag="PKM27_17235"
     CDS             complement(4154..4741)
                     /locus_tag="PKM27_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09068.1"
                     /translation="MKTIINAVLILLSLSFLYMLYISIKEPLEFQAARDSRTNEVINR
                     LKTIRECQEMYRDIYGSYAATFEELANGLKTGQFRDIKVIGDPDDPEFTGKITYDTLF
                     TSAIDSIKRMGLNLDSLAFVPYANGARFDLKTDTIKQGNIMIDVIEVGIQRKAFMGKF
                     ADISYKKFDNRYDPESRVKFGDLNSPSVTGNWEGK"
     gene            4826..5866
                     /gene="tsaD"
                     /locus_tag="PKM27_17240"
     CDS             4826..5866
                     /gene="tsaD"
                     /locus_tag="PKM27_17240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNR09069.1"
                     /translation="MRKKVFLASLTRFFLPVILAIESSCDETSVAVIEDGRVLSNIIA
                     NQTIHYEYGGVVPELASRAHQENIVPATTRALEYAGKSLEDLDAVGITIGPGLLGSLL
                     VGVSFAKSLAYSLSLPLIQVNHMQAHVLAHFAEPPYPSFPFICLTVSGGHTQIVLVRS
                     PAQMEVVGETLDDAAGEAFDKAGKIFGLGYPAGPLIDRHAKNHPPEIHFPHPQVPGLN
                     FSFSGLKTAVLYHVQQHTAQNPDYVTKHIGVLCASLQQTIISILMKKLVQAAKELNIR
                     HVAIAGGVSANSGLRQALQDAGIQHGWETFIPKFEYCTDNAAMIGIAAHFKYQNGDFS
                     SLDITPSSRLPF"
     gene            complement(5923..6648)
                     /locus_tag="PKM27_17245"
     CDS             complement(5923..6648)
                     /locus_tag="PKM27_17245"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNR09070.1"
                     /translation="MMSLWQYAASSIVLMLGGALIAALMTRKPGRLIMQDLLSLSGAF
                     LFGVLILELMPDIFHEEGHSVAILFIAGFFLQTGMDYWTSGIEHGHLHVPPTAGKTFV
                     FSLFIGLGLHAILDGLPFAGIDFHGHQHGSIYLAVLLHKLVEGFTLFILLGMLGYTTR
                     VTWLHVSLFSFITPLGMVLFSSIPFLLDHYHQVLAFAGGSLLHVAVTILFESEHKHHH
                     GMPVRKLISILIGLLMAGLTVMG"
     gene            complement(6645..>7107)
                     /locus_tag="PKM27_17250"
     CDS             complement(6645..>7107)
                     /locus_tag="PKM27_17250"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNR09071.1"
                     /translation="NQYVWGPLYVFLVGFILFNYGKQGGLYLLLLICTLSVSDMVSSR
                     LIKKNVERLRPCNQVELEGQRRVLVRCGSGYSFTSSHATNHFTVASFMVASSFNFLGN
                     LRFLFLLWAACIAYAQVYVGVHFPLDILAGSLLGTFIGYTGSKISNFFLYR"
     assembly_gap    7108..7117
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7598..8638
                     /gene="galE"
                     /locus_tag="PKM27_17255"
     CDS             7598..8638
                     /gene="galE"
                     /locus_tag="PKM27_17255"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNR09072.1"
                     /translation="MPIKPILKVLVTGASGYIGSHTAIDLLQHGHEVIGLDNYVNSSP
                     RVFDRIEKISGKPCLSYQADLRDKDSLGRIFDAHPRLDAIMHFAALKSVDESVVHPDL
                     YYENNVSGLINLIHFQKKHRVPRLIFSSSCTVYGNADQLPVTESTPWKQAESPYGLTK
                     QLGEMILQDFTRHAGSMSCVSLRYFNPAGAHPGGHLGEAATKEVRNLIPLIMETGAGK
                     RPFLSIYGNDYPTRDGTNVRDFIHIMDLARAHTLAMDYLGNLPAGTYEVFNLGTGRGV
                     TILEAIEAFNRVRGEKLPVKWAGRRPGDVVAIYADYQKAKNLLGWQPAYTMEDIMAHA
                     WAWEKNKSELIS"
     gene            complement(8631..>10857)
                     /locus_tag="PKM27_17260"
     CDS             complement(8631..>10857)
                     /locus_tag="PKM27_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR09073.1"
                     /translation="IPVKFLGRWMRVIYFLILALHLQAQGGLKGWLVDEQKMPLEGAT
                     LAWKNMSAHAVSDSAGYFQIQRLRSDSILVIQYVGFKTLEYKIPEGQHEIRLTLSENI
                     VLQQVEVRAKANDSFTPLSSNMNKEVISMRELRKAPCCNLSESFETSAVVDANYTNAA
                     LGTREIEMLGIRGIYSQIMLDSRPTMYNFASPFAFDFIPGPWLKGIQVSKGAGTVANG
                     AEGLAGQINVDLVDPRDGPRLFVNAYANHRSRYEMNVVTNKRLGEKWSTGTLLHANQD
                     QHHQDKNEDAFLDAPQKKQINAMQRFHYYGLKWETQINVHGIWDKREGGQTRHPSPDH
                     QEELLLYEHEQKRLEIFGHVGYVGFKDPGRSIGFQWHLQGHEYDAAYARIANGSDRSA
                     YANLLYQEKFGSEKHSLLSGLSMRRSVTDEGFSDFLFNRKEFIPGLFSEYSYNADVTE
                     SIFGFTLGMRLDRYGDYGVHATPRFSMRYQVDEYGTLRASAGRAYRMPNIISDNLHFI
                     GMGRYFQVPSIGLDIANNYGLSYIQKIYLGGPTEWNISADFYHTFFKKQNIQDIESET
                     RYVINRYVEHGSRINYFLLQNQFQMNANFGFKLAYKYTDARYRLADGQDLISRLYLPK
                     HRALFTLDLNSNESVWLGNVTVQWVGPQRMLDISEYPTPGSAGQPDQSPSYLMVNFHI
                     SRNIGKWELYTGMENATNYVQEYQIVSPTATGSRYFDATRLYAPNMGMRWYGGIKWLL
                     N"
BASE COUNT         2599 a         2877 c         2699 g         2672 t
ORIGIN      
        1 cgcggatggt gggcatgcgg gggtcgtccc agcctttgac gtattgttca ttcaccagct
       61 gcatcagttt gcgtttgctc attacggtat aactcaggtt gagccgggca aattcatact
      121 ggcgggaggg aaagatgccc agctgatcga tataccattc gtacagggga cggtgatttt
      181 caaattcaag ggtgcagatg ctgtgcgtga tcttttcaat gctgtcgcac tgcccatggg
      241 caaaatcata catcgggtaa atgacccatt tgtcgccggt gcggtggtga tgcgcttttt
      301 tgatgcggta catcaccggg tccctcatat gcatgttggg cgaagccatg tcgatctttg
      361 cccggagcac gcattcccct tcctcgaaaa ccccctcctt cattttgctg aacaagtcca
      421 ggttttcttc cacgctccgg atgcggtaag ggctttctat accgggctgg gtgggactgc
      481 ctttctggtc cgcgatggct tcggcggagg aatggtccac ataagccttt cctttccgga
      541 tcaggagcag ggcgaattca tacagctggt caaaataatc cgaggcaaaa aactggcggt
      601 ccccccagtc aaagcccagc cagcgaacgt ccgccatgat ggattccacg tattccactt
      661 cttccgtctc gggattggta tcgtcgaagc gaaggttggt ctggccgccg tatttgtccg
      721 ccacgccgaa attcaggcaa atggatttgg catgcccgat atgcaaataa ccattgggtt
      781 cgggtgggaa ccgggtcaat atcctgccgc cgtgtttacc ttcttcgagg tcccgttcga
      841 tgatttcctc aataaaattc aatgactctg acatagctga atattacatt ctttcgggca
      901 tatcgatgcc cagcaaatta aacgaatctt cgagcacccg gcccacgcaa tcgctgagca
      961 ccagtcggaa ttgaagggcc accggatcgt ccgcccggag gataggtact tcatgataga
     1021 accggtggta tgccttggcc aggttgtaac tgaaattggc cacttccgat ggatcgtaag
     1081 cttcagcggc cttagccacc gtctggggat aggtttccat caggctgatc agttcttttt
     1141 cttcttcggc cagtccctta tagtcctggt aaccggcttc gctccgttcc cccgattttc
     1201 tcaggaccga ctggatgcgc acataggcgt tctgtacata aggcccggtc tggccctgca
     1261 ggtcaaggga ttctttggga tcgaagagca tgcgtttttt gggatgcact ttgagcataa
     1321 aaaatttaat ggctcccagg gcgatcttgc cgatgatctc ctcttgtttg gcccggtcaa
     1381 aatcacccag gtcccccctc tcttccgccg tcttttccgc ttcacggatg atttccgcca
     1441 ccaggtcgtc cgcatccacg acggtgcctt ccctggattt catcttgcct tcgggaagtt
     1501 ccaccatgcc gtaggaaaga tggtacaaac cgtctgcgta gggttctttg aatcgtttta
     1561 aaatttcaaa gaggacctta aaatgataat cctgttcgtc agccaccaca tagatgagtt
     1621 tgttcatttt gaacttttcg taacgcatct gggccgtgcc gaggtcctgg gtaatgtaaa
     1681 cggaagtgcc gtcgctgcgc aaaaccactt tctggtccat accggcgtcc gagaggtcta
     1741 tccagaccga ctggtccggt tttgaataaa agatgcccat ttccaggcct ttccgcaccg
     1801 cctcttttcc aagcaggtag gtagtggatt cgaaatcggt atggtcaaaa cggaccccca
     1861 tccgatcata ggtggcatta aatccgtcat agacccattg attcatccgg ctccagagtt
     1921 ccagggtcgc cggatcatgg ttttcccaga gcaacagcat ttcgcgggcc tccctgccga
     1981 gcaggctgta agcattaaaa tactggtttt tgaattccct gaaaaaagcc ggttcatcca
     2041 gctctttgtc cttccggttt tcaaattcct gatgtgcctc gggcgtggat tgccaggcaa
     2101 gatattcttt ggcaaattct ttttcgaaaa ggacataata atccccgaca aaatgatcgc
     2161 ctttgatccc cgtcgtggag ggggtggccc ctccggccca tttactccag gcgaggatgg
     2221 acttgcatat ggcaatgccc cggtcattga tcacctggac ccgccggacc tcatagccgg
     2281 agatctccag catgcgggct gtggaagagc ccagcagcat atttcggata tggcccaggt
     2341 ggaggggttt attggtattt ggggatgaaa actcaaccag gacggtttga tttttgggtt
     2401 tgccgtgacc aaatttcgga tcgccctgaa tgtgttgaag ggtccgggtc catatcgcgt
     2461 catccagggt catattgagg aaaccctgga ccacttcgaa ggaagtaatt aaacgatttt
     2521 ccagcatttt attaccgatc tcctgcgcga cggtcatggg gtttttcctg agtttcccgg
     2581 ccaccgggaa taccgtgagc gtataatccc ctttaaaatc cttttttgtc ggcacgacca
     2641 ggagctcgga agcgggccat tccacagggt agagttcctc gatggcctga gccagctggt
     2701 tttgcaataa ttctttgatc ataaaaaggt gggcaaaggt acaaaatcca ttgcaggatg
     2761 attttttgcg cttcctccga tctccaagaa ttcttgcccc cttaacctca acctcaacct
     2821 caacctcaac ctcaaccgca accttaacct taaccttaac ctcaacctta acctcaacct
     2881 taaccttaac ctcaaccctc ctcaacaggc cattcaaaaa aagaaaacag cgtggaaccg
     2941 tatttccttt cttccttaaa acaagggtgt ttggtaaaat tataattggg attgtgttcc
     3001 aggataaaaa cgccgccggg tgaaagcagt cggttttcaa aaatcatatc cgggatcctg
     3061 cccagttcct tcaatgcata gggggggcct gcgaatatat aattaaacgt cttctgcgcc
     3121 ctgccgataa acttgatcgc atcatcctgg acgatggtca ggcaatcgtc cactttccaa
     3181 tcctgggagg ttttttttac aaaatcaata caaccccgga acaaatcgac ataggtgacc
     3241 tctttacacc cccggctgat cagttcaaaa ctgtgggaac cgcttccccc aaacaggtcg
     3301 agggcatgga gggattcgaa atccagccgg ttttccagga tattaaacag cccttcccgg
     3361 gagaaatcgg tggtgggccg ggtaggccag tttttagcag gtggattcaa cctgcggcct
     3421 ttcaaccgtc ccgctatgat ccgcatgcgc cctggatgat ttccttgatc atttccggcc
     3481 ctggtggctg cagcaggctg acttccggaa cgaatttttt caatatccgg agatgggccg
     3541 tcgaggggtc attggtccag accttgaagg cctgctgatg gcttcggtat ttctctacca
     3601 gttttagggt aaaataggtg ctgtcgtccg gagaaggcag ccggagtttc gaggcttcct
     3661 tgagtacttt gtttacctgg acgaggttgt acatcgtatc ctgtatgcgc agagtaaaca
     3721 ggccggtatg atgccggtca aaccaggcct gggcataggc agccaggcag gtcatccagt
     3781 gcaaggtctc ggatgcatga tatggtccga atggagccgg gaggtaatag atgattttgg
     3841 aataggtgga cggccaggtc tcggtccgga ccgccgactc accgtatccg ggataaggca
     3901 gaatggcctg gagcaggttt aacccggatt tttcatcata aaggctctcc ggcaccagct
     3961 ggaatggagt atccagaatg attcgcatcc ctccctgggc tgcatgctct tccagggaag
     4021 ggaacaaatc cgcccgggaa taaagcgtca aggggttgat cccttccttt aaaataaaaa
     4081 agatgtcatc gaatacgatc catgacatct ttttacgggt taatggttta gtcggtatct
     4141 ccggatgttc cacttatttg ccttcccagt tgccggtaac acttggtgaa ttaaggtccc
     4201 cgaatttcac cctggattcc gggtcatacc ggttgtcaaa ttttttgtag gaaatatcag
     4261 caaacttgcc catgaaggct ttgcgctgaa tgcctacctc gatgacatcg atcatgatat
     4321 tcccttgttt tatagtatcg gtcttgaggt caaaacgggc cccgttggcg taaggtacaa
     4381 aagccaggga atcgaggttt aatcccatcc gtttgatgga gtcaatcgcc gaggtaaaaa
     4441 gggtgtcgta ggtgatttta ccggtaaatt ccggatcgtc cgggtccccg atcaccttga
     4501 tatccctgaa ttggccggtt ttgaggccgt tggccagttc ttcaaaagtc gctgcatagc
     4561 tgccatatat gtcgcggtac atttcctggc actcgcggat ggttttgagg cgattgatca
     4621 cttcgttcgt cctgctgtcc cttgcagcct ggaattcaag cggttctttg atgctgatat
     4681 agagcatata taaaaacgac aggctcaaaa gaatcaatac agcattgata atggtcttca
     4741 tattataaaa ttcttttttc tgaggtcaca agtatagcac tttttgaagg aaactaatat
     4801 cccaactgtt aacaaaatta aatagatgag aaaaaaagta tttttggcgt ctttaacccg
     4861 cttttttttg ccggtcatcc ttgcgataga atccagttgc gacgaaactt ctgtcgcggt
     4921 catcgaagac gggcgggtgc tctccaacat cattgccaac cagacgatac actatgaata
     4981 cggaggggtg gtgcctgagc tcgcctcccg ggcccaccag gaaaatattg tgcctgccac
     5041 cacccgggcc ctggagtatg ccggcaaatc cctggaagat ctggatgccg ttgggatcac
     5101 tatcgggccc ggcttactgg gttccttgct ggtcggggtt tcatttgcaa aaagcctggc
     5161 ttattcgctt tcccttcccc tcatccaggt caaccatatg caagcccatg tcctggccca
     5221 ttttgcagag cccccctatc cttccttccc ctttatttgc cttacggttt caggcggaca
     5281 tacccagatc gtcctggtcc gaagcccggc gcaaatggag gtcgtcggtg aaaccctgga
     5341 cgatgccgcc ggagaagctt ttgataaagc gggaaaaatc tttgggctcg gttaccccgc
     5401 cggtcccctg atcgaccgtc atgctaaaaa ccatccaccg gaaatccatt ttccccaccc
     5461 ccaggtaccc ggattgaact tcagtttcag tggattaaaa acagcggttt tgtaccacgt
     5521 ccagcaacat accgcgcaaa atccggacta tgtaacaaaa catatcggcg tgctttgcgc
     5581 ttccctgcag cagaccatta tcagcatcct catgaagaaa ctggtccagg ccgccaagga
     5641 actgaatatc cgccacgtcg ccattgccgg gggggtgtcg gcaaacagcg gcctcaggca
     5701 ggcgcttcag gacgcgggca ttcaacacgg atgggaaacc tttatcccga aatttgaata
     5761 ttgcaccgat aacgcggcca tgatcggcat tgccgctcat tttaaatacc agaatggaga
     5821 tttcagcagc ctggacatca ccccctcctc ccgcctccca ttttaatcac ccctcaatcc
     5881 tcaaccctca cccctcaatc ctcaacccac aatcctcacc cctcaaccca tcaccgtcaa
     5941 tcctgccatt aacaggccga tcaggatgct gatgagtttt ctgaccggca ttccgtggtg
     6001 gtgtttatgc tcggattcaa acaaaatggt tacggccaca tgcaggagag accctcccgc
     6061 aaaagcaagg acctggtgat aatggtcaag caggaaagga atggaggaaa aaagcaccat
     6121 gcccaaaggc gtgataaaag aaaataagga tacgtggagc caggtgaccc gggtcgtata
     6181 acccagcata ccgagcagga tgaacaaggt gaaaccctcc accagtttgt gcaataaaac
     6241 cgccaaatag atggatccat gctgatgtcc gtgaaaatcg atgccggcaa agggcaagcc
     6301 gtccaggatg gcatgcaatc ccagaccgat gaaaagggag aaaacaaaag ttttacctgc
     6361 cgtgggcggg acatgcagat gtccgtgttc gatgccgctc gtccaataat ccattccggt
     6421 ctgaaggaaa aaaccggcga taaacaggat ggccaccgaa tgtccttcct cgtggaaaat
     6481 atcgggcatc agttccagga tcagtacccc aaacaaaaat gcgccgctca gggaaagcag
     6541 gtcctgcatg atcaggcgac ccggtttccg ggtcatgagc gccgcgatca acgccccgcc
     6601 cagcatcagc actatggagg aggccgcgta ttgccagaga ctcatcatcg atacaggaag
     6661 aaatttgaaa ttttagaacc ggtataccct ataaaagtgc ccagcaggct gcctgccagt
     6721 atatccagcg gaaaatggac tccgacatat acctgggcat aggcgatgca ggctgcccat
     6781 agcagaaata aaaaacgaag gttacccagg aaattaaagc tggaagccac catgaaggag
     6841 gccacggtaa aatgattggt cgcgtgcgag gaggtaaaac tgtatccgga cccgcagcgc
     6901 acaaggaccc tgcgctgtcc ttccagttca acctggttgc agggcctcag cctttccaca
     6961 tttttcttga tcaggcggct gctgaccatg tccgagaccg aaagcgtgca gatcaacaac
     7021 aacaggtaca ggcctccctg cttaccgtaa ttgaatagga taaaacccac caggaagaca
     7081 tacaaggggc cccatacgta ttgattgnnn nnnnnnngca taaacttcat gaatacatcc
     7141 agccaggctg aggtccaaac ctggttgatc agatgaaaca accggtagtc gatattcagc
     7201 aattcctcca tgaagcaccg gcaaaattaa tttaaagttt tgcttccagg ggttttatcc
     7261 gcagatgggg gtatgaccat cccccttctt cgggagcggt tttgcatttt caaataaacc
     7321 aggcttgctc ctgaaagtgt gggatagtca tgcacacttc gactcattgt acggaaaacc
     7381 aattaatcgg aaatcactag atttgagtaa aatataaacc gcacggaacg ctgataaaaa
     7441 caggaggcac cgggactttt attcactttg ctcctctgtg tccttcttgc gatttctgca
     7501 ttctcttcgg tttagaattt ccgccgtata tgatctcttc cgttctcagc aatcattcaa
     7561 gacctgttgc catggcttct gcaaaacgtc aggtaccttg ccaatcaaac ccattttgaa
     7621 agtactggtc accggggcat cgggatatat agggagccat acggccatcg acctgctcca
     7681 gcacggccac gaggtgatcg gcctcgacaa ttatgtcaat tcctctcccc gggtatttga
     7741 ccggattgaa aaaatctccg gcaagccctg cctttcttat caggccgacc tcagggacaa
     7801 ggacagcctg ggtaggatct tcgatgcgca cccccggctg gatgccatca tgcattttgc
     7861 cgcactgaaa agtgtggatg aatcggtggt ccacccagac ctctattatg aaaacaatgt
     7921 cagcggcctg atcaacctga tccatttcca gaaaaaacat cgtgtgcccc ggttgatctt
     7981 ttcctcctcc tgcaccgtat atggcaatgc cgaccagctc cccgtcacgg aatccacccc
     8041 ctggaaacaa gccgaatccc cttatggtct gaccaaacag cttggtgaaa tgatcctgca
     8101 ggattttacc cggcatgccg gatccatgag ttgtgtatcc ctgcgatatt ttaatccggc
     8161 cggcgcgcac ccgggcgggc atttgggaga agccgccacc aaagaagtgc gcaacctgat
     8221 ccccctgatc atggagaccg gtgccggcaa aaggcctttc ctgagcattt acggcaatga
     8281 ctatcctacg cgcgatggaa ccaacgtaag ggattttatc cacatcatgg acctggcccg
     8341 ggcccatacc ctggctatgg attacctggg aaatctccct gcaggaacct atgaagtatt
     8401 caacctgggc acgggaaggg gcgtgaccat tttggaggcc atcgaagcct tcaaccgggt
     8461 aaggggagaa aaattgcccg taaaatgggc cggcagaagg cccggggacg tagtcgccat
     8521 ctatgcggat taccagaagg caaaaaatct cttgggctgg caaccggctt atacgatgga
     8581 ggacatcatg gcccatgcct gggcctggga gaaaaacaag tcggaattga tcagttgagc
     8641 agccacttga ttccaccata ccagcgcatt cccatattgg gtgcgtagag gcgggtggcg
     8701 tcaaaatacc gcgagcccgt ggcggttgga cttacgatct ggtattcctg gacataattg
     8761 gtggcatttt ccattcccgt ataaagttcc catttgccaa tattccggct gatgtggaag
     8821 ttgaccatca ggtaggacgg cgattgatcc ggctgacctg cactgccggg ggtcgggtat
     8881 tctgaaatat ccagcatccg ctggggcccc acccactgaa ccgtgacatt ccccagccac
     8941 acgctttcgt tcgaattgag atcaagggtg aataaggcac ggtgcttcgg taggtacagc
     9001 ctggaaatca ggtcctggcc gtcagccagc cggtaccggg catcggtata tttataagcc
     9061 agcttgaagc caaaattggc attcatctgg aactggttct ggagaaggaa atagttgatc
     9121 cgggaaccat gctccacata gcggttgatg acatacctgg tttccgattc gatatcctgg
     9181 atgttttgtt tcttaaaaaa agtatggtag aaatcggcgg atatattcca ttccgtcggg
     9241 cctcccaggt aaattttctg tatatagctc agaccataat tgttggcgat atccagacca
     9301 atcgagggca cctggaaata ccggcccatc ccgataaaat gaagattgtc tgaaatgata
     9361 ttgggcatcc ggtaagcacg tccggccgaa gcccgcaggg taccatactc atccacctgg
     9421 tatctcatgg aaaaacgggg cgtggcgtgg accccgtaat ccccgtagcg gtccaggcgc
     9481 atgccaaggg taaagccgaa tatcgattcg gtcacgtcgg cattgtagga atattcgctg
     9541 aacaggcccg gaataaattc cttccggttg aacaggaaat cggagaaccc ttcatcggtc
     9601 acgctccgcc tcatgctcag tccgctcagc aggctgtgtt tctctgaacc gaatttttcc
     9661 tggtacaaca ggttggcata ggccgaccgg tcgctgccgt tggctatcct ggcataggcg
     9721 gcatcgtatt catggccctg aagatgccat tgaaaaccga tggaacggcc cgggtcctta
     9781 aaacccacat acccgacatg gccgaaaatt tccagtcgtt tttgctcgtg ttcatagagg
     9841 agcaattctt cctggtggtc cgggctggga tgccgggtct ggcccccctc ccttttgtcc
     9901 cagatgccgt gcacattaat ctgggtctcc catttcaggc cataataatg gaaccgctgc
     9961 atggcattga tctgcttttt ctgcggcgcg tcgaggaagg catcttcatt tttgtcctgg
    10021 tggtgttggt cctggttggc atgcaggagg gtgcctgtgc tccatttttc accaagcctt
    10081 ttgttggtca ccacgttcat ttcatagcgg cttcggtgat tggcataggc gtttacaaac
    10141 aatctcggcc cgtccctggg atccaccagg tccacattga tctggccggc caggccttcg
    10201 gcgccattgg ctacggttcc ggctcccttg ctcacctgga tccctttgag ccaggggcca
    10261 ggaataaaat caaaggcgaa aggggatgcg aaattgtaca tcgtgggccg cgaatccagc
    10321 atgatctggg aatagattcc ccggatgccc agcatttcga tctcccgggt gcccagggcc
    10381 gcattcgtat aattggcatc cactaccgcg ctggtttcaa aactttcgga gaggttgcag
    10441 caaggcgctt tacgcagttc ccgcatgctg atcacctcct tattcatatt gctgctcagg
    10501 ggggtgaaac tgtcattggc tttggcccgg acttccactt gctgcaggac aatgttttca
    10561 ctcagcgtaa ggcgtatttc atgttgccct tcaggaatct tatattcaag cgttttaaag
    10621 ccgacatact ggatcaccag gatgctgtcc gatctgagcc tttggatctg gaaatatccc
    10681 gcgctgtcgg agacggcatg ggccgacatg tttttccagg caagggtagc cccttccagc
    10741 ggcatttttt gttcatcgac cagccatccc tttaatcccc cctgggcttg aagatgcaag
    10801 gccaggatca gaaaataaat aaccctcatc catctcccca aaaacttaac cggtatc
//
LOCUS       DAOQXT010000135        10660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6661_length_10660_cov_6.915134, whole genome shotgun sequence.
ACCESSION   DAOQXT010000135 DAOQXT010000000
VERSION     DAOQXT010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6661_length_10660_cov_6.915134"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..511
                     /locus_tag="PKM27_17265"
     CDS             119..511
                     /locus_tag="PKM27_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNR09074.1"
                     /translation="MKKQIKPTDSELAILQILWQKGPQPVRAVNEFLSRKSGGEIGYT
                     TTLKLMQIMTEKGLLRRNTDQRSHIYEAAVQEHETQQNLLGDFLQATYRGSASTLVMQ
                     ALGSYHASKDELRMIKALIDNLESNNKK"
     gene            523..2748
                     /locus_tag="PKM27_17270"
     CDS             523..2748
                     /locus_tag="PKM27_17270"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNR09075.1"
                     /translation="MFSNLFSNAPDLMQAWVWAMIHSVWIGLVLAFVFYSWLGLSPRA
                     SARSKYRVGMSLMLLMPAFVLLAFLLSIPEPQTAGLVQAVVGDPIHAVQAPVLENKAY
                     ASMASLWMSKIENHVQLIFLIWSLGVILFSLRMALGYHQIGRLRKEVQAIELPALVRQ
                     FSDLVRRSGIRKKITLASSLRVDVPVTLGHLKPVILLPIGLINHLSPEETKAILAHEI
                     AHIMRRDYLQNVLISTLEILFFYHPSVWWFSATIKTIREQCCDDLALELGAEPIALSK
                     ALIRLEEQATAPTFALAFAHKNQLLHRVQRLFKNPIRNEYRMSRGQAPLLVCGLALVW
                     LFSSPLESLGNYDNAMRLSKSFIWDTGRAAADTVKPKSRIEKISRDNGKQKVEISLEN
                     KEIKELKVDDKVIPPSEYEKYKGETDALKKELSEIEMPERRGRVFRDYEPGEYHFNYN
                     FNFDDSLGIVDIPTVFPKGSLHPRVYSIRPRVEVKPGERRAFTFRDRDGHERSLFFQD
                     GDRIRGMRHNGTKWIFEGDSSGMRLDGDRLIIEDKDGNIIIDLGKEKNWPGGRMRMFG
                     PGTFEFNLDKSSDLFHGRLEDLENLHLKIPDESMEKAMKHLDREKLRIKEGQHEMREK
                     LRMSEEQLKELMEDMGHGFREIPDFEWEEPLRRSGKVFSLGRKNNLQQAVERQLDRDN
                     LVEKGKSYEFKITSKDLRINGKKQDAQRYKQFKSLVEEHTGIELEEGSVIQFSGSLDQ
                     K"
     gene            2796..3509
                     /locus_tag="PKM27_17275"
     CDS             2796..3509
                     /locus_tag="PKM27_17275"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNR09076.1"
                     /translation="MKYLYLAIVLFALPLAAWSQEAKPKKEKKVVTIRKSTDKEGNEV
                     IEKKILEGDAATKESDELKSDGRTIRITIDSSGEKEEIIIDNEIRIGEDGDPDQIRIL
                     KESRTPYTERTVTVKKNPDGSVEIKEQSGAGLDPRIHIRVDSTEAHKPSIGVMLNEEL
                     SISGLVKGGAAEAAGLAKGDVLTHLDGTFIDDYDHLKQMISKKKVGEQVTLTYLRDRK
                     EMKTTLTLKGGSTRIYFNR"
     gene            complement(4162..5823)
                     /gene="rny"
                     /locus_tag="PKM27_17280"
     CDS             complement(4162..5823)
                     /gene="rny"
                     /locus_tag="PKM27_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNR09077.1"
                     /translation="MDWILALIAGLAGGSGAAYVILNNIFKKTRQKQLEQSNQKSDLI
                     LEQAKIAAKRITDEAEVNAEKALAKAESRNEQIKQKKIQEAKDHFNSKRAEFEKEKAA
                     HLVEMKEREMIVVNGENEYKRKMAEIKAKAEQVESKESELNALRENLEKQLKIVNRRK
                     EELDAATEKQVKALENIARLSEQEAKEQLIEAIRAKAESEAMSFVKDAAEQAKVNANK
                     EAKKIVIQTIQRMASEYTIENTVSVFHLENDDVKGQIIGREGRNIRALEAATGAEIVV
                     DDTPEAIVISSFDPIRREICRLALQRLVADGRIHPARIEEVVAKVKKQLEEQIREIGE
                     RTVIDLDIHGLDPYLVWMVGRMRFRSSYGQNLLKHSIETANLCATLSAELGLNTKLVK
                     LAKRAGLLHDIGKVSEEESELSHAILGMKLCEKYHEHPAIINAVGAHHDEIEMNSIIS
                     PIIQACDAISGARPGARREILESYLKRIGELEELAMSYDGVQKAYALQAGRELRVIVE
                     SEKVSDGFADELAYMISQRIQNEMQYPGQVRVTVIREKRATAIAK"
     gene            complement(5881..6156)
                     /locus_tag="PKM27_17285"
     CDS             complement(5881..6156)
                     /locus_tag="PKM27_17285"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNR09078.1"
                     /translation="MKTLQVMIEGRTYPLKVNEEDAASMLKIVEEVNESLKSLHVSYP
                     QKDRQDCMAMALLTYAVKLYESRADSLSPDIQEKLEVMDRLLDEVVL"
     gene            complement(6167..6457)
                     /locus_tag="PKM27_17290"
     CDS             complement(6167..6457)
                     /locus_tag="PKM27_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09079.1"
                     /translation="MKAVPAILDRMEEKIRVVSQKVELLQQENKLLHDENHKLNKELK
                     LREKLIREMEDQRTLFSDQEDTAKVSSRKMEQTLDQYIKQVDQCIDLVKNIG"
     gene            complement(6505..8952)
                     /gene="pheT"
                     /locus_tag="PKM27_17295"
     CDS             complement(6505..8952)
                     /gene="pheT"
                     /locus_tag="PKM27_17295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNR09080.1"
                     /translation="MNISYNLLQNFLPLSEEPEALAETLTNLGLAVDAYAPYESIKGG
                     LAGVVVGKVVECIRHPNADRLSLTRVDLGTGALHQIVCGASNVAAGQTVLVATPGTKL
                     YNTKGEPLEIRKGKIRGETSDGMICAADELGLSDDHSGIMVIPDHHPAGAPATDVISV
                     YRDTVFEIDLTPNRSDATSHLGVAKDLAAYFAVQTGKDPGFKAWGDGPLPELHLEKPV
                     RVIVEDPVRCPRYSGISLSGVTIRESPDWLKERLNAIGVRPISNVVDVTNWILHLYGQ
                     PLHAFDLDKIGGGMVRIKTLPRGTKFVSLDAVERSLHEDDLMICDADSQPLCIAGVFG
                     GIGSGISADTKNIFLESAHFSASSVRRTSMRHVLRTDAATRFEKGTDPNITIKALREA
                     ARLIVELAGGYLSSDFVDVYERPIEPVQVELSLNKVRSVIGQEIPDARIKKILDALDM
                     PVLGEHGDRLRIAVPTNKSDVKRDIDVIEEILRIHGFNAVPMPAKMEVSFAISSDASR
                     KEKLREGTAAFLSSLGFHEIMGMSLMESRILSGSGLDAKDLVLVNNTSNINLDVMRPD
                     MLRSGLQSIQYNQSRQQGMLKFYEFGFTYHRSPDAKWPFRETPSLAVFIYGPVDQEHW
                     DLKDARQGFFFLKGLIHQLLAKWQLGTSRENDLDHPDFEFAREISIEGTPVAVYGKVQ
                     ESLGKVFDLRADLYYAFFHWDKIWEGFNRPRPEKVQIPGKYPSVRRDLAFVVDKAMAW
                     QQIRGVLEDLKIPDLVHFDLFDMFENEDRIGPGKKSLAISLIFENPNQTLRDADLERS
                     MNLILQALIQKTRASVR"
     gene            complement(9138..10007)
                     /locus_tag="PKM27_17300"
     CDS             complement(9138..10007)
                     /locus_tag="PKM27_17300"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HNR09081.1"
                     /translation="MTYLSKTICLLAFSNFLAPVLAQEGSVNKEKYHWGIISQDTSYG
                     YAGVVRSGNTLYLSGVTAPGNFPTQVQSVYTTLENNLKRFGATFRNVVKENLFTTSID
                     SMKKYSDIRKPFYKNDYPAASWIGIKELFAPDRMLEVELVAHLPQPDPKFSLPASDWP
                     GLILPGTWIMNTLRGQILEKWESTSQDVLTGRSYRIRNQDTTLMETVRLIRENQEVFY
                     IPTVEGQNEGKPVRFRMTSSHNNTFVFENPDHDYPKRVVYDFKNREVIHAWIDAGESQ
                     KEMRSDYFFHRIH"
     gene            complement(10048..>10660)
                     /locus_tag="PKM27_17305"
     CDS             complement(10048..>10660)
                     /locus_tag="PKM27_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09082.1"
                     /translation="NALVPRLDGDYSPAGVLNHMILSLSRNKKTIPQYLRLALEDASV
                     HQPGHVSLAMSCFWEGEKRLGLLEGILGTEPGFIQGREVVRVRYDPDRISESKIVAEA
                     KKQDCADAVFTRDQPGFKPDRQPQYYLLHSPFKFVPMLPLQASRVNSALGLGKDPLEY
                     LSPRQVGFYHFFKSFPEKGNPQAYRQADFKTHWETASALLRQS"
BASE COUNT         2599 a         2649 c         2770 g         2642 t
ORIGIN      
        1 aaagggttaa tattttcata aaatacgaat ttgttcgtaa taaagattga cagggtgggt
       61 ccggcgttgt atcattgcat tacgaaagaa ttcgtattag ttattccaat aaaagccgat
      121 gaaaaaacaa atcaaaccaa cagacagtga actggcgatc ctgcagatcc tttggcagaa
      181 agggccgcaa ccggttaggg cggtgaatga attcctgtcc cgcaagagcg ggggagaaat
      241 cgggtatacc accacgctca agctgatgca gatcatgacg gaaaagggtt tgctcaggcg
      301 gaatacggac cagagaagcc atatttatga agcggccgtg caggaacacg aaacccagca
      361 aaacctgctc ggcgattttc tccaggccac ctaccgcggt tcggcgagta cgctggtgat
      421 gcaggccctg ggcagttacc atgccagcaa ggacgaactg cgcatgatca aagccctgat
      481 tgacaacctc gaatccaata ataaaaaata aaaactcctt tcatgttctc caatttattt
      541 tccaatgcac cggacctcat gcaggcctgg gtatgggcta tgattcattc cgtatggatc
      601 ggcctggttc tggcatttgt cttctattcc tggctggggc ttagtccgcg tgccagcgca
      661 agatccaaat acagggtggg aatgtccctg atgcttttga tgcccgcctt tgttctgctt
      721 gcctttttat tatccattcc tgaaccgcag acggccgggc ttgttcaggc ggtggttggg
      781 gacccaatcc atgccgtgca ggctccggtc ctggagaaca aggcctatgc atccatggct
      841 tcgctctgga tgtcaaaaat tgaaaaccat gtccagctga tattcctgat ctggagcctc
      901 ggggtgatcc ttttcagtct tcggatggct cttggatatc atcagatcgg gcgtttgcgc
      961 aaggaggttc aagccattga acttccggca ctggtccggc aattcagtga cctggtccgg
     1021 cgttccggaa tccggaaaaa aatcaccctg gcaagcagcc tccgggtgga tgtcccggtc
     1081 accctgggcc acctcaagcc ggtcatcctg ctgcctatcg gcctgatcaa ccacctgagc
     1141 cctgaagaaa ccaaggcgat cctggcccat gaaatcgccc atattatgcg cagggattac
     1201 ctgcaaaatg tgctgatctc caccctggaa atcctgtttt tctaccatcc gtcagtctgg
     1261 tggttttccg ccacgatcaa aaccatccgt gaacaatgct gcgacgatct ggctttggaa
     1321 ctgggcgcgg aacccattgc tttatccaaa gccctgatcc gcctggaaga gcaagctacc
     1381 gcacccacct tcgccctggc ctttgcccac aagaaccagt tgttgcaccg ggtgcaacgt
     1441 ttgtttaaaa atccgatccg aaacgaatac cggatgagcc ggggacaggc cccgctgctg
     1501 gtctgcgggc ttgccctggt ctggttgttt tccagtccgc tggaaagcct gggcaattat
     1561 gacaatgcta tgcggttgtc taaaagtttt atttgggaca ctggccgggc ggcagcggac
     1621 actgtaaaac ccaaatcgcg tattgaaaaa atatccaggg acaacggaaa gcagaaagtc
     1681 gagatcagct tggaaaataa ggaaatcaaa gagttgaagg tggacgacaa ggtgatcccg
     1741 ccctcggagt atgaaaaata caaaggggag accgatgcgc tgaagaaaga gctttccgaa
     1801 attgaaatgc cagagcgcag ggggagggtg ttccgtgatt atgaacccgg tgaataccat
     1861 ttcaattata atttcaattt tgacgatagc ctgggtatag tagatatccc aacggttttc
     1921 cctaaaggca gtttacatcc cagggtatat tccatcaggc caagggtaga ggtcaaaccg
     1981 ggtgaacggc gtgcgtttac cttccgggac agggatggac atgaaaggtc cctgtttttc
     2041 caggatggtg acagaatccg gggaatgcgc cacaatggta ccaaatggat ctttgaaggg
     2101 gactcctcgg gcatgaggct ggatggcgac cggctgatca ttgaggacaa agacgggaat
     2161 atcatcatcg acctgggtaa agagaaaaac tggccgggcg ggcgaatgcg gatgtttgga
     2221 ccaggaacat ttgaatttaa tctcgacaag tccagtgact tgttccacgg tcgcctggaa
     2281 gatctggaaa acctccacct caaaatcccg gatgaatcca tggaaaaagc aatgaaacac
     2341 ctggaccgtg aaaaactgag aataaaagaa ggccagcacg aaatgaggga aaaactgagg
     2401 atgagcgagg aacagctaaa agaactcatg gaagatatgg gacatggatt cagggaaatt
     2461 ccggattttg aatgggaaga accgctgcgg cgatcgggaa aagtttttag cctgggccgg
     2521 aagaataacc tgcagcaagc cgtcgaaagg caactggatc gggacaatct ggtggaaaaa
     2581 ggcaaatcct atgaatttaa aatcacgtcc aaggacctgc ggatcaatgg taaaaaacag
     2641 gatgcccagc gctacaaaca atttaaatcc ctggtggagg aacatacggg catcgaactg
     2701 gaagaaggat cggtgattca gttcagcggt tccctcgatc agaaatagcc ctgagtaaaa
     2761 acctttattt ttgtccaagc ttataaaaaa caaccatgaa atatttgtat ctcgcgatcg
     2821 ttctgtttgc cctgcctctg gctgcctggt cccaggaggc caaacccaaa aaggaaaaaa
     2881 aggtggtgac catccgtaaa agcacggata aggagggcaa tgaagtcatt gaaaaaaaga
     2941 tcctggaagg agacgccgcc acgaaagaaa gcgatgaact caaaagcgat ggccggacga
     3001 tccgaatcac catagacagc agcggggaaa aagaagagat catcatcgac aacgaaatcc
     3061 ggattgggga ggacggggac ccggaccaga tccgcattct gaaggaaagc cgaacgcctt
     3121 atacggaacg caccgtaacc gtaaagaaaa atccggacgg gagcgttgaa atcaaagaac
     3181 agtccggggc aggcctggat ccgcggatac acatccgggt ggacagcacg gaggcccata
     3241 aacccagtat cggggtgatg ctcaacgaag aactcagcat atccggcttg gtcaaagggg
     3301 gtgcagcgga agccgcaggc cttgccaaag gggacgtact gactcacctg gacggtacct
     3361 ttatcgatga ttacgatcat ctcaaacaaa tgatctcaaa gaaaaaagtg ggcgaacagg
     3421 ttaccctcac ttatttacgc gaccgtaagg aaatgaaaac caccctcacc ctaaaagggg
     3481 gatccacccg catctacttt aacaggtaat attaatgcaa ggttaaattt tataggaagt
     3541 cctgttaaga tgaatcagga tgcaaccttt gacagtaata ttacatctct atttcagtgc
     3601 tgacccatta gctaaataag accaaaaaca aatttactct aactggctcc gaagaataaa
     3661 atcttcgttc cagaatgcag acaaagcaga ccggattggt agtctttccg gggtgagctt
     3721 actttgcctg ttcctcctta cggatttagt gagtcttttc atatagccct ggtttttttc
     3781 taaagagaat aaatccgggg ttttttattt aattaaggat taaagaatta aaataattaa
     3841 agtgacaacc actagcaacg caggggtggc tctaactagg atgttttggg gagcttagct
     3901 ctgacctgga gaatatcatt cgctgataat taattatgcg atcgggtgag ctaaacttcg
     3961 acttaacgaa aataagcggg tggaagtcac ccaggaatcg tcccgtggct tcgcaaagtg
     4021 attgccgcat gctgggcttg gggggcaagc tcgaagtatc aaccactggc aacgctaaaa
     4081 tggctttcac ttaccaactt gccaactagc caactagcca actagccaac tagtcaactg
     4141 acaaactagc caactaactt cctacttcgc aatcgcagtc gcccttttct cccggatcac
     4201 cgtcacccgc acctgcccgg ggtattgcat ttcattctgg atgcgctggg agatcatata
     4261 ggccagttcg tcggcaaagc cgtccgaaac cttttcgctt tccacgatca cgcggagttc
     4321 gcggccggcc tggagggcat aagctttttg caccccgtca taagacatgg ccagttcttc
     4381 gagctcaccg attcgtttga gataggattc caggatctcg cggcgggcgc ccggccgggc
     4441 gccggaaatg gcatcgcagg cctggatgat gggcgaaatg atgctgttca tttcgatttc
     4501 atcgtggtgg gcgccaacgg cgttgatgat ggcgggatgc tcgtgatatt tttcacagag
     4561 tttcattccc aggatggcat gggacagttc gctttcctcc tcactcactt tgccgatgtc
     4621 gtgcagcagg cccgcccttt tggccaactt gaccagcttg gtgttgaggc ccagttcagc
     4681 gcttagggtt gcgcaaaggt ttgcggtttc gatggaatgt ttgagcaggt tctggccata
     4741 ggaggagcgg aaacgcatgc gtcccaccat ccacacgagg tatgggtcca ggccatggat
     4801 atcgaggtct ataaccgttc tttcaccgat ttcgcgaatc tgttcttcca gttgtttttt
     4861 aaccttggcc accacttctt cgatgcgtgc cgggtggatc cggccgtccg ctacgagccg
     4921 ttgaagggcc aggcggcaga tctcccggcg gatcggatca aaggaggaga tgacgatggc
     4981 ttccggggtg tcgtcgacga cgatctcggc gccggtggcc gcttccaggg cccggatatt
     5041 ccgtccttcc cggccgatga tctggccttt gacgtcatcg ttttccagat ggaatacgga
     5101 taccgtgttt tcaatggtgt attctgaggc catacgctgg atggtctgga tcacgatctt
     5161 tttagcttcc ttgttggcgt tgaccttggc ctgttctgcg gcatctttga cgaaagacat
     5221 ggcttccgat tcggctttgg cccggatggc ttcgatcagt tgttctttgg cttcctgttc
     5281 ggaaaggcgg gcgatgtttt caagcgcttt gacctgtttt tcagtagcgg catccagttc
     5341 ctctttcctg cggttcacga ttttcagttg tttttcgagg ttttcacgga gggcgttcag
     5401 ttcactctct ttggactcga cctgttcggc tttggctttg atctcggcca tttttcgttt
     5461 atactcattt tcgccattga cgacgatcat ttcccgttct ttcatttcca ccagatgcgc
     5521 ggctttttcc ttttcaaatt cagcccgttt gctgttgaag tggtctttgg cctcctggat
     5581 ctttttttgt ttgatttgtt cgttgcggct ttcggctttg gccagggcct tttcggcgtt
     5641 gacttccgct tcgtcggtaa tgcgtttggc ggcgattttt gcctgttcca ggatgaggtc
     5701 ggatttctgg ttggattgct ccagctgttt ctgccgggtc tttttaaata tattgttgag
     5761 aatcacatag gcggctccgc ttcctccggc cagccctgcg atcaatgcta atatccagtc
     5821 catggtatgg ttcggttttt aataagtaaa taaataaaag agaacaaaga ggaaagaaaa
     5881 tcagagtacc acctcatcca gcaaccggtc catgacctcg agcttttcct gaatatcggg
     5941 agacaggctg tctgccctgg attcgtagag tttgacggca taagtcaaaa gggccatagc
     6001 catacaatcc tgccggtctt tctgaggata agagacatgt aaagatttca gggattcatt
     6061 gacttcttcc acgattttca gcatcgacgc cgcgtcttcc tcgttgactt tcaacgggta
     6121 agtgcggcct tcgatcatga cttgaagtgt tttcatccct gaggtactat cctatatttt
     6181 tgaccaggtc tatacactga tccacctgtt tgatgtattg atcgagggtt tgttccattt
     6241 tccgcgaact cacctttgcc gtgtcttcct ggtctgaaaa aagcgtacgc tgatcttcca
     6301 tttccctgat gagtttttct ctcagtttca gctctttgtt caatttgtga ttttcatcat
     6361 gcagcagctt gttttcctgt tgcagtaact caactttttg agaaaccacc cgtatttttt
     6421 cctccatgcg atccaatatg gcaggaactg ctttcacaaa tatattatat ttatattata
     6481 tataaaatac taatcttctt tctactacct cactgaggcc ctggtttttt gtataagggc
     6541 ctgcaaaatc aggttcatag aacgttccag atcagcatcc cggagggtct gatttgggtt
     6601 ttcaaatata agacttattg ccaaagattt tttgcccggg ccgatccggt cctcattttc
     6661 aaacatgtca aaaagatcaa aatgcacgag atcagggatt ttaaggtcct cgagcacgcc
     6721 ccggatctgc tgccaggcca tcgccttatc caccacgaaa gcaaggtccc gccgcaccga
     6781 aggatatttt ccagggattt gcaccttttc gggacgggga cggttgaacc cttcccatat
     6841 cttgtcccag tggaaaaatg cataatacaa gtcggcccgc aaatcgaaaa ccttgccaag
     6901 ggactcctgc acttttccat aaaccgcaac gggggttcct tcgatggaga tttcccgggc
     6961 aaattcaaag tccggatggt ctaaatcgtt ttcccgcgag gtccccagct gccattttgc
     7021 caagagttgg tggatcaggc ctttgagaaa gaagaatccc tggcgtgcgt ccttaaggtc
     7081 ccaatgttcc tggtcgaccg ggccatagat aaagaccgcg agcgaaggcg tttcccgaaa
     7141 aggccacttg gcatccgggg accggtgata ggtgaagcca aattcataaa atttaagcat
     7201 gccctgctga cggctctggt tgtattggat gctttgaagg ccgcttctta gcatatccgg
     7261 ccgcatgaca tcgaggttaa tattgctggt attattgacc aagaccaggt cttttgcatc
     7321 caggcccgaa ccgctcaaaa tcctggattc catgagcgac atgcccatga tttcatgaaa
     7381 gcccaggctg ctcaggaaag ccgcggtccc ttcccgcagt ttttctttcc ggctcgcatc
     7441 cgatgaaatg gcaaaggata cttccatttt agccggcatg ggcacggcat taaaaccatg
     7501 gatccgcagg atctcttcga tgacatcgat gtcccgcttc acgtcggatt tgttggtggg
     7561 cacggcgatc ctcagccggt ccccatgttc gcccagcacc ggcatgtcca gggcatccag
     7621 tatttttttg atccgggcat cggggatttc ctgtccgatc accgacctca ccttattgag
     7681 gctcaattcg acctgcacgg gttctatcgg cctttcataa acatccacga agtcagagga
     7741 gaggtaccct ccagccagtt ccacgatcag cctggccgct tcccggagcg cttttatagt
     7801 aatattcgga tcggtcccct tttcaaaacg ggtcgccgca tcggtccgca atacatggcg
     7861 catgctggtc ctgcggacgg aagaagcgga gaaatgagcg ctttccagga aaatattttt
     7921 tgtgtcggcg ctgatgccgg agccaattcc tccaaatacc cctgcaatgc aaaggggttg
     7981 ggaatccgca tcacagatca tcaggtcatc ttcgtgcagg ctgcgttcga ccgcatccag
     8041 gcttacaaat tttgtacccc ggggcagggt tttaatcctg accatcccgc cgccgatctt
     8101 gtcgaggtca aaagcatgga ggggctggcc atacaggtgc aggatccagt ttgtgacgtc
     8161 caccacattg ctgattggcc ttacgccgat ggcattgagc ctttccttaa gccaatccgg
     8221 agattcgcga atggtgaccc cggacaggct gatcccgctg tatctcggac aacggacagg
     8281 atcttcgacg atcacccgga ccggtttctc caaatgcaat tccggcaatg ggccgtctcc
     8341 ccaggctttg aatccggggt ctttaccggt ttggactgca aaataggcgg ccaggtcctt
     8401 tgcgaccccc agatggctgg tggcgtcgga acggttgggc gtgaggtcta tttcaaatac
     8461 ggtatcgcga tacacggaaa tgacgtcggt ggctggggct ccggccgggt gatggtccgg
     8521 aatgaccatg atcccgctgt ggtcatccga caagccgagt tcatcagccg cgcagatcat
     8581 gccgtctgag gtttcacccc ggatcttacc tttacggatt tcaagcggtt cacccttggt
     8641 attgtacaat ttggtcccag gggttgccac gagcacagtc tgtcccgcgg ccacattgga
     8701 ggccccgcat acgatctgat gaagggcacc ggtgcccagg tccacccggg tcagggaaag
     8761 ccggtctgca ttgggatgcc ggatgcattc cacgaccttt ccaacaacca cgccagccaa
     8821 gccgccctta atactttcat aaggggcata agcatccacc gccaggccca gattggtcaa
     8881 ggtttccgcc agggcttcgg gctcctcgga caggggtaaa aaattttgca gaagattata
     8941 agaaatattc ataaaaaacg cggcaagata cggaggatag agctaaaccc aagtggataa
     9001 ctgggcatag gtagaacgag gtaataatta accgctaaaa acgcaaagag cgcaaagcga
     9061 tggccgagat aatagtaatg aacgctgcac acctattgcg tgcacgctcc ccctaagctt
     9121 aaaaaatgcg ataaggatca atgaattctg tggaaaaaat aatcggaacg catttctttc
     9181 tgactttctc ccgcgtcgat ccaggcatgg atgacctccc tgtttttgaa atcatagacc
     9241 actcttttgg gataatcgtg atccgggttt tcaaacacaa aggtgttgtt gtgggatgaa
     9301 gtcatccgga agcggacggg cttgccctca ttctgtcctt ccacggtagg tatataaaac
     9361 acctcctggt tttccctgat caggcggacc gtctccatca gggtggtgtc ttggttcctg
     9421 atccggtagg acctgcccgt caggacatcc tgggacgtgg attcccattt ttcgaggatt
     9481 tgcccgcgca gggtattcat gatccaggtc ccgggtaaaa taagccccgg ccagtccgag
     9541 gccggcaggg aaaatttggg atcgggctgg gggagatggg ccaccagttc cacttccagc
     9601 atgcggtccg gtgcaaacag ttccttgatt ccgatccagc tcgccgcagg gtaatcgttt
     9661 ttgtagaaag gcttgcggat atcactgtat tttttcatcg aatcgatgct ggtagtaaac
     9721 aggttttcct tgaccacatt gcgaaaggtg gccccgaacc gtttcaggtt gttttccagg
     9781 gtggtgtata cggattggac ctgggtggga aaattacccg gcgcggtcac cccggaaaga
     9841 taaagggtgt tgcccgaacg gacgacgccc gcataaccat aactggtatc ctgactaatg
     9901 atgccccaat gatatttttc tttgttcaca gagccctcct gggcgaggac gggggcaagg
     9961 aaattgctga aggccagcag gcaaatcgtc tttgacaggt aggtcataat ctcggtttta
    10021 ttggggtaag gaatatatta attcggatca gctctgacgg agcagggccg aagcggtttc
    10081 ccaatgtgtt ttgaagtccg cctggcgata ggcttgcgga tttccctttt ccgggaagga
    10141 tttaaaaaag tgatagaacc cgacctgcct cggggacaag tattccaggg gatctttacc
    10201 caggcccagg gcgctgttga cccggcttgc ctgcaaggga agcatgggca cgaatttaaa
    10261 aggggaatga agcaggtaat attggggttg gcggtccggt ttgaagccag gctggtcccg
    10321 ggtaaaaacg gcatccgcac agtcctgttt tttggcttca gccactattt tggactcgct
    10381 gatccggtcc gggtcatagc gtacccggac cacttcacgg ccttggatga atcccggttc
    10441 cgtgccaagg atgccttcca ggaggccgag tcttttttcc ccttcccaaa aacaagacat
    10501 agccaggctc acatgtcccg gctgatggac cgaggcgtct tccaaagcaa gccggaggta
    10561 ttgcgggatg gtttttttgt tccggctgag ggatagtatc atatggttta atacacctgc
    10621 cggggagtaa tcaccatcca ggcggggaac cagggcattc
//
LOCUS       DAOQXT010000136        10523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6775_length_10523_cov_6.998089, whole genome shotgun sequence.
ACCESSION   DAOQXT010000136 DAOQXT010000000
VERSION     DAOQXT010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10523
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6775_length_10523_cov_6.998089"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1484)
                     /locus_tag="PKM27_17310"
     CDS             complement(222..1484)
                     /locus_tag="PKM27_17310"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNR09083.1"
                     /translation="MIQILFILLTRFFTAPAQDQDFVPRNLDTIYSPDIATVRCHVAG
                     LPLSLPVLELDGGSTLSISFDDLRSGAHRYKYEVIHMDMNWKKDELEEYDYIDGFNRS
                     EIRNYAYSTNRNLDYTHYRFELPNNDLRLKISGNYLLHVYEELRRGQRTYLFTRRILV
                     VEPKVKVNTYFVRPTDGLKYRTHQEMDFEISVKGFQVYNSRQEITCAVMQNGRWATAI
                     EGLKQNRDKPEHLVFDYIDKVSFPAGKDFRFFDTRSLEFRTRFVKKITENQNEIIVTL
                     FDDKTREFFNFVDERDANGNFLIDNQDRPNANLSAEYVWVDFTLKSPEFFDQKVYLMG
                     AFTDWQCKEPYLMKYDAESQAYLGSALLKQGYYNYGYVVMGKGGDFSASPVDGDWYET
                     ENEYQVLVYYRPFGARYDRLIAFHTTRP"
     gene            complement(1592..3376)
                     /gene="glgP"
                     /locus_tag="PKM27_17315"
     CDS             complement(1592..3376)
                     /gene="glgP"
                     /locus_tag="PKM27_17315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNR09084.1"
                     /translation="MTSFQVLAGIPAPPYSRWKYRGFVSPEGVFPILPIFAAFQTAFD
                     MSFNNYKVPYTPGALYSSKVAYFSMEFAIHQALKIYSGGLGFLAGSHMRSAYELKQNL
                     IGIGILWKYGYYDQARNQDQTLQPVWYEKNYYFLEDTNIKFQITIHGAPVWVKAWYLS
                     PYIFRTAPVFFLSTDLPENDYISQTICHRLYDANPSTKLAQFILLGVGGATLLDHLGF
                     NPDFYHLNEAHAASAIFHLMKKLKSKEEIKKKLVFTTHTPEEAGNEKHDIYLCERMSY
                     FGDLSLDEVRELTGIHDEVFNHSLVALRFAKVANAVSQLHGKVSRKMWAPYEDICEIK
                     SITNAQNYNYWADKKLYAAMEEEDHGSFDERKRYLQKRSFDNVSDQCGKLYDPDVLTL
                     VWARRFANYKRPDLLTQDMDRFEALISSKKYPIQIIWAGKPYPQDYAAISTFNNLVNL
                     SKKYKNLAVLTGYELNLSKRLKQAADVWLNTPRVPREASGTSGMTAAMSGAVNFSTFD
                     GWICEFAEHGKNSFIVPPGEYENMKVHEQDMYDMEKIFDILESEILPAFYDRPHDWRA
                     IQYQGMKDVRYRFESKRMADEYYRIMYQ"
     gene            3426..4499
                     /gene="leuB"
                     /locus_tag="PKM27_17320"
     CDS             3426..4499
                     /gene="leuB"
                     /locus_tag="PKM27_17320"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNR09085.1"
                     /translation="MAEKHILIVPGDGIGQEVTTAGKNVLDKIAEKFGHRFTYDAALM
                     GHAAIEATGNPLPDETLAKMRASDAVLFGAVGHPKYDNDPTAKVRPEQGLLKMRKELG
                     LYANLRPIKLFDDLLGASSIKPEILRGSDILFFRELTGDIYFGEKGRKDNGDTAYDLA
                     QYSRYEVDRIARKAFDAARARKKKLCSVDKANVLETSRLWREVVQQVATEYPDIQVEH
                     QFVDSMAMMLIKDPRRYDVVVTANLFGDILTDEASQIAGSMGMLASASIGDGTGVYEP
                     IHGSAHDITGKGVANPLASILSAALMLEISFNLKPEAEAVVRAVDEVLKAGWRTRDIA
                     GPDTPPEKILGTEKMGAEVLRRI"
     gene            5362..6330
                     /locus_tag="PKM27_17325"
     CDS             5362..6330
                     /locus_tag="PKM27_17325"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNR09086.1"
                     /translation="MKYQSILLLSLLLGVYCITIPIHVQAQAVKPWWPHPQWGAGDQA
                     GASNWITPEKILQAVSLVRTGKTYEMGQVYENTMPTGNRTYKLVIPSFPTHGPIGEEK
                     VVFNDEFLSAEIGQVGTQFDGLGHVGMQMPRSDGSVTEVFYNGFTKEDMRNPYGLLKL
                     GIEHIKPIITRGLLIDIAGLRGVSTVPDSLELSLAEVRSALARQGIAESWIQPGDALF
                     FNFGWWRHWPGPEATHAGKRPKINMDVVRWLIDQKPSMVGSDAILDGTVFNVHTELTM
                     KQGVFNLEWMNFKEMSEDKAYQFLFIFTPIRFKGATGSPGRPVGIR"
     gene            6549..8516
                     /locus_tag="PKM27_17330"
     CDS             6549..8516
                     /locus_tag="PKM27_17330"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNR09087.1"
                     /translation="MRIPKDKVTAGTLLIALGIIYGDIGTSPLYAFKAILGEHVIEKM
                     LVYGGVSCIFWTLVIQTSIKYIWLTLRADNDGEGGIFSLYTLVRRFGKKLVIPTMIGA
                     AALLADGIITPPISIMSAVEGLEAIPGLQHIPIIPIVIFLISVFFFFQRFGTQKVGSL
                     FGPVMLVWFTMLLTLGLAQVVQHPDILRSLHPWYAYQLLSKYPGGFWILGAVFLATTG
                     AEALYFDLGHCGIKNIRITWVFVKTCLVFNYLGQAAWAMQHLDTGLDGRNPFYQLMPA
                     WFVMVGIVIATAAAIIASQAVISGSYTLVSEAVNLNFWPRVATRQPSETKGQIYVPSV
                     NLLLWIGCMLVVLYFQSSSKMEAAYGLAITIAMMMTTTLLNNFLLYRLKWNKIAVFLV
                     IGFFGLIELSFFAANLRKFPEGGFITVVIGSVFFAIMYIIYFGRKISNQLTRWVNLED
                     YNQSLKDLSEDRTISKFATHLIYLTKANTIQEIELKIMNSIFAKKPKRADVYWFLHLH
                     RTTSPYTLEYDIHELLDDKVIKININIGFRIQPLTELYFKQIVRDLVNNQELNLHIRP
                     DGSTRYNTDPDFKFIVIEKYISVENVFSFREGILLNGFFFLKNLGLKDENAFGLDKSD
                     VEIESIPLVFHPIENINLRRLVKEKEPVAES"
     gene            8660..>10523
                     /locus_tag="PKM27_17335"
     CDS             8660..>10523
                     /locus_tag="PKM27_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HNR09088.1"
                     /translation="MKYLSFFIFSLVMQAAGYADDGYRLWLKYDLIEDPALLKMYRSG
                     IRSIRLQAAETALTASAVRELTTGLMGLLGKAINSTNKPASSAQIILSLSPPGGVINH
                     EEGYRVVKDKNNIRIEALTDRGLLYGSFALLAYLQQARPLHALHLESSPKIKLRLLNH
                     WDNPNGTIERGYAGSSLWKWYDLPDQPDPRYRDYARANASIGINGTVVNNVNASARFL
                     SREYLVKVAALASIFRQYGIKTYLSIRFTAPTYLDGFKTADPLDPAVRQWWKEKASEI
                     YSLIPDFGGFLVKANSEGEPGPQDYNRTHADGANMLAEALATHGGIVMWRAFVYKANP
                     NNDRASESYNEFKPLDGQFLDNVLVQVKNGPIDFQPREPFHPLFGAMPRTPLMMEFQI
                     TQEYLGFATHWVYLANMFKEVLDSDTYSKGPGSTVARIIDGTVDRHKLNGIAGVANTG
                     SDRNWCGHIGNQANWYAFGRLAWDPDLQTGFIAEDWIRMTLTRNTADVDVIKKIMMDS
                     HEACVDYMTPLGLHHIMGESIHFGPQPWLARSGRADWTSTYYHRADSIGLGFDRTEGG
                     SIAIGLYAPEVQAQWKDPETIPLKYLLWFHHVNWNKKLNTGNTLWEELCKRYYSG"
BASE COUNT         2507 a         2670 c         2747 g         2599 t
ORIGIN      
        1 cgccaggtga aagccaaggg cggctgccag cagctgccgc gcgggctggc gctaggggaa
       61 gtttaattgg cttccacctg ctgtcgcgcg ggctggcgct aggtgaaagc caagatggct
      121 tccacctgct tccgcatggg ctggtgccag atgaaagtca aggtggcttc cacccgctgt
      181 cgcgcgagct agcgccaggt gaaagccacc tgggactgca gttagggtcg tgtggtgtga
      241 aaggcgatca ggcggtcgta ccttgccccg aagggccggt aatataccag gacctggtat
      301 tcgttttcgg tttcatacca atccccgtcc accggtgaag cgctgaaatc ccctcctttg
      361 cccatgacca catagccata attataatat ccctgtttga gcagggccga gccgagatag
      421 gcctggcttt ccgcatcgta cttcatgaga tagggctctt tgcattgcca atcggtaaaa
      481 gcccccatca ggtaaacttt ctggtcaaaa aactcaggac tttttaaggt aaaatccacc
      541 caaacgtatt cggcggagag gttggcattg ggccggtcct ggttgtcaat caggaaattt
      601 ccattggcat ccctttcgtc tacaaaattg aaaaattcgc gggtcttgtc gtcaaaaagg
      661 gtgacgatga tctcgttttg gttttcagtg atctttttta cgaagcgggt cctgaattcc
      721 agggaccggg tatcaaaaaa acggaagtcc ttgcccgcgg gaaaactgac cttgtcgata
      781 tagtcaaaaa cgagatgctc gggcttgtcg cggttctgtt tgaggccttc aatggcggtg
      841 gcccagcggc cgttttgcat cacggcgcag gtgatctcct gccttgaatt gtaaacctgg
      901 aatcccttca cggaaatctc aaaatccatt tcctggtggg tccggtattt caggccatcg
      961 gtaggccgga caaaataggt attgactttc actttgggct ccacgacgag gatgcgccgg
     1021 gtaaacaggt aggtgcgctg ccccctgcgc aattcttcat agacatgcag caggtagttg
     1081 ccagagatct tcaggcggag gtcgttgttg ggcaattcga aacgataatg ggtataatcc
     1141 aggttccggt tggtggaata ggcatagttc cggatctcag accggttgaa gccgtcgatg
     1201 tagtcgtatt cttccagttc gtccttcttc cagttcatgt ccatgtggat gacctcgtac
     1261 ttgtaccggt gggcaccgct gcgcaggtcg tcgaaggaaa tactcagggt ggagcccccg
     1321 tccagttcga gaacgggcaa actgagtggc aggcccgcta catgacagcg taccgtagcg
     1381 atatccgggg aatagatcgt gtccaggttt cgggggacaa aatcctgatc ctgcgcaggt
     1441 gcggtgaaga accgggtcaa caggataaat aaaatctgga tcaaatcagg tcctattaat
     1501 tcgtaaggca aagatccgca tatttcgggg atcctccggt aaaacggtgg ggttgtccaa
     1561 gaagttacac ccctggcctt ccggtctccg gttattgata catgatccgg taatactcgt
     1621 cggccatgcg tttgctttcg aagcggtaac gcacgtcttt catgccctgg tattgaatgg
     1681 ccctccagtc atggggccgg tcgtaaaagg cgggcaggat ctccgattcc aggatgtcaa
     1741 atattttttc catgtcgtac atatcctgct catgcacttt catattttca tattcaccgg
     1801 gcggaacaat aaaactgttt ttaccatgtt cggcaaattc gcagatccac ccgtcgaagg
     1861 tgctgaaatt caccgcgccg ctcatggccg cggtcatgcc gctggttccg ctggcttccc
     1921 ggggcacccg gggggtattg agccacacgt cggccgcttg cttgagcctt ttactcaggt
     1981 tcagttcata tccggtgagg acggccaggt ttttgtattt tttactcagg ttgaccaggt
     2041 tgttgaaggt gctgatcgcg gcgtaatcct ggggataggg cttccccgcc cagatgatct
     2101 ggatcgggta ttttttgctc gaaatcaggg cttcaaaccg gtccatgtcc tgggtcagta
     2161 gatcgggccg cttgtaattg gcaaagcggc gcgcccaaac cagggtgagc acatccgggt
     2221 cgtaaagttt tccgcactgg tcactgacgt tgtcgaagct ccgcttttga agataccgtt
     2281 tgcgttcgtc aaaactgccg tggtcttctt cctccatggc ggcataaagt tttttatcgg
     2341 cccagtagtt gtaattctgg gcattggtga tggatttgat ttcacagatg tcctcgtagg
     2401 gggcccacat tttacggctc accttaccgt ggagctggct tacggcattg gccaccttgg
     2461 caaaacgaag ggccacgagg gaatgattaa atacttcgtc gtgaatgccc gtcagttccc
     2521 gcacctcgtc gagggaaagg tccccgaaat aagacattct ttcacagagg tagatatcgt
     2581 gcttttcatt gccggcttct tcaggagtgt gggtggtgaa caccagtttc tttttaattt
     2641 cttcttttga cttcagcttt ttcatcaggt ggaatatggc gctggccgcg tgcgcttcgt
     2701 tgaggtgata aaaatccgga ttgaagccca ggtggtccag caaggtggcg ccgcccacgc
     2761 ccaggaggat aaactgagcc agtttggtcg aggggttggc atcataaagg cggtggcaga
     2821 tggtctggga gatgtagtcg ttctcgggaa ggtctgtact cagaaaaaat accggagcgg
     2881 tccggaaaat ataaggggag aggtaccagg ctttgaccca taccggggcg ccgtggatgg
     2941 tgatctggaa tttgatgttc gtgtcttcca ggaagtaata gttcttttca taccagaccg
     3001 gctgaagagt ctggtcctgg ttgcgggcct ggtcataata cccgtatttc cagaggatgc
     3061 cgatgccgat caggttttgt tttaattcat aagcgctccg catatgcgat cctgccaaaa
     3121 agccgaggcc cccgctgtaa attttgaggg cctggtgaat ggcaaattcc atggagaaat
     3181 aagccacttt gctggaataa agggccccgg gcgtataggg tactttgtaa ttgttaaaac
     3241 tcatgtcaaa agcggtttga aaggcggcaa atataggaag aatgggaaaa acgccttccg
     3301 gggacacaaa tccccggtat ttccacctgg aataaggggg ggctgggata ccggccagga
     3361 cttgaaagct ggtcaaatcc ttgtattttg cagcccttta aaaacgatac cgaaagaaat
     3421 accccatggc ggaaaaacac attctcattg tacccggcga tggcattggc caggaagtca
     3481 ccactgccgg aaaaaacgtg ttggataaaa ttgcggaaaa atttggccac cggtttacct
     3541 atgacgccgc cctgatgggt catgcggcta ttgaagccac cggcaatccg ttgcccgatg
     3601 aaaccctggc taaaatgcgg gcttccgatg ccgtgttgtt tggcgcggtc ggccatccga
     3661 aatatgacaa tgatcccacg gccaaagtca ggccggaaca gggcttgctg aagatgcgaa
     3721 aggaactggg gctctatgcc aatctacgcc cgatcaaact gtttgatgac ctcctgggcg
     3781 cttccagcat caaacccgaa atccttcgcg gatccgacat cttgtttttc cgggaactca
     3841 ccggggatat ttattttggt gaaaaaggaa gaaaggacaa cggggatacc gcttatgacc
     3901 tcgctcagta tagccggtac gaagtggatc gcatcgcccg caaagctttt gatgcagccc
     3961 gtgcccgaaa gaaaaaactt tgttcggtgg ataaggccaa tgtactggag accagccggc
     4021 tttggcggga agtggtgcag caggtcgcga cggaataccc ggacatccag gtggaacacc
     4081 agtttgtcga ttccatggcc atgatgctga tcaaggatcc tcgacgctac gatgtggtgg
     4141 tgaccgccaa tctcttcggg gatattctta cggatgaagc ttcccagatc gcaggatcca
     4201 tgggcatgct ggcctcggct tccatcgggg atggcaccgg ggtgtatgaa cccatacatg
     4261 gttcggcgca cgacatcacc ggaaaagggg tggccaatcc cctggcatcc atcttatcgg
     4321 ccgccctcat gctcgaaata tcctttaatc tcaaaccgga agcggaagcc gtggtccgag
     4381 cggtggacga ggttttaaag gcaggttggc gcacccggga tattgccggg ccggacacgc
     4441 caccggagaa aatactgggg acggaaaaga tgggggccga ggtgcttcgc cggatctgac
     4501 cggttcccac ggagcttagc cattcgtttt tcccacaacg acgcaaagaa cacaatctgt
     4561 gtgcgcatgt ctgtaaatag tcgtgttaaa tcccaaatca cttctgttcg gacgaagctt
     4621 ggcttcggcc catcccaccc gttcggtaga ccccttggat gtagcttagc ttcggtcccc
     4681 caattcggac ttcagcccag aagacatata tgatatcgtt ttgcaaaaat tgccctccaa
     4741 cattgtgtct ttaggcacgt caaatgggta gatcaaaagg gcaggtcatt tcgtcccatc
     4801 cgggacgata tcaaaatcta aaattttatg ctacccgtat gaaatcccta cgggatggaa
     4861 tatccggaca cagcctgcct caggtttggg tgaagcttta tatctcccgc gaagtttgag
     4921 gcagttgggt gaactgcaac gtcaggagag ataactttgg taaatggctg gtataagaag
     4981 ccttcttcgt aattggaaat ttaaatttct ccaccaaagt cttatttata taaattacag
     5041 attaccaatt gtttggcatt tagttatata ggagacacaa agaacaaaac ctaggtggtg
     5101 cgggcaagtc tgtaaatggt cttgttaaaa cccaaatcac ctaatagaaa gcctattgcc
     5161 cgttcgggtt aaatggtttc atgcacttag cttgggattt tcggaaaagt cgaggaagac
     5221 ttcatccctg cctttggctt cacactggta ttttaaaacg cattcgacag ggaatagcga
     5281 tttatccaat ttttacatta ttcggagatt ccggaaaatc gaatggtgaa aattttaaat
     5341 tcatatttaa atacgaggaa tatgaaatat caatcaatcc ttcttttatc tcttctgctg
     5401 ggcgtttact gtataacaat ccccatccac gtccaggcgc aggcggtaaa accctggtgg
     5461 ccgcatccgc aatggggcgc cggtgaccag gcaggggctt ccaactggat cacgcccgaa
     5521 aaaatactcc aggccgttag cctggtccgg acgggaaaga cctatgaaat ggggcaggtg
     5581 tatgaaaaca ccatgcccac gggcaaccgg acctataaac tcgtgatccc ttccttcccc
     5641 acccatggcc cgatcgggga agaaaaagtt gtgtttaacg atgaattttt atcagccgaa
     5701 atcggccagg tggggaccca gtttgacggc ctgggccacg tgggaatgca gatgccaaga
     5761 tccgacgggt ctgtcacgga ggttttttac aacggtttca ccaaggagga tatgcgtaat
     5821 ccttatggcc tgctcaaact gggtatcgaa cacatcaagc ccatcatcac ccggggactc
     5881 ctgatcgaca tcgccggatt gagaggggta tccaccgtgc ccgattctct cgaattgagc
     5941 ctggctgagg tccggtcggc cctggcccgg caggggatcg cggaatcctg gattcagccg
     6001 ggggacgccc tattttttaa tttcggctgg tggaggcatt ggcccggacc cgaggcaacc
     6061 catgccggca aacggccgaa aataaatatg gatgtagtcc ggtggctcat cgaccaaaaa
     6121 cccagcatgg tgggttccga tgccatcctc gacggcacag tctttaacgt tcataccgag
     6181 ctgaccatga aacagggggt cttcaatctc gaatggatga atttcaagga gatgtccgaa
     6241 gacaaagcct accagttctt attcattttt actcctatcc ggtttaaagg ggcaacgggc
     6301 tctccgggga gaccggtggg gatcagatag gaccccgcag gatatttatt aatttcgaat
     6361 tctatttcct gaaatcattc cgcgatttta ttcagagtga ttattttcgc cggccatgag
     6421 gtcaatcctt tcactaggat ccggttatcc cggtttttca catgaaaact atttctcatg
     6481 cattatcttt agacccctgg attcaaatga acccctgttt ttcaactttc ctgctttaat
     6541 tggttaccat gcgcattcca aaagataagg tgacggccgg caccctgctg atcgccctgg
     6601 gaatcattta cggagatatc gggaccagtc ctttatatgc ttttaaagcc atcctgggcg
     6661 aacacgtcat agaaaaaatg ctggtgtacg gaggggtgtc ctgcattttc tggaccctgg
     6721 ttatccagac ctccattaaa tacatctggc tgaccctgag ggcggataac gatggggaag
     6781 ggggcatttt ttccctttat accctggtcc ggcgctttgg taaaaaactg gtgatcccga
     6841 cgatgatcgg agcggcggcc ctgcttgccg acggcatcat caccccgccc atctcgatca
     6901 tgtccgcagt ggaaggactg gaagccatac cggggctgca acatatcccg atcatcccga
     6961 tcgtgatttt cctgattagc gtttttttct ttttccagcg gtttggcacg cagaaagtgg
     7021 ggtcgttgtt cggcccggtc atgctggttt ggtttaccat gttgctgacc ctgggattgg
     7081 cacaggtcgt tcagcatccg gatatactca ggagcctcca tccgtggtac gcttaccagc
     7141 ttctgagcaa atatccaggc ggtttctgga tccttggtgc tgtattcctg gccactaccg
     7201 gcgcggaagc cctgtatttc gatctcgggc attgcggcat caaaaatatc cggatcacct
     7261 gggtattcgt taaaacctgc ctggtgttca attatctcgg gcaggccgcc tgggcgatgc
     7321 aacatctgga caccggcctg gatggaagaa atccctttta ccagttgatg ccggcctggt
     7381 tcgtaatggt gggcatcgtg atcgctacag cagctgccat catcgcatcc caggcggtaa
     7441 tcagcggatc ttataccctg gtgagtgaag cggtcaacct gaatttctgg ccccgtgtgg
     7501 ccacacggca accctccgaa accaaaggcc agatctatgt gcccagtgtc aatcttttgc
     7561 tctggatagg gtgcatgctg gtcgtgctgt attttcaatc ttcttccaag atggaagcgg
     7621 cctacggact ggcgattacc atagccatga tgatgaccac caccctgctc aacaattttt
     7681 tattgtacag gcttaaatgg aacaagatcg ccgtattcct ggtgatcggg tttttcggcc
     7741 tgattgaact ttcgtttttc gcggccaatc tgaggaagtt tcccgaaggg ggttttatca
     7801 ccgtggtcat cgggagcgtg ttttttgcga tcatgtacat catttatttc ggccgaaaga
     7861 tcagcaatca gctgacccgg tgggtcaacc tggaagatta caaccagtcc ctgaaagacc
     7921 tgagcgagga ccgcaccata tctaaatttg ccacccatct gatctacctg accaaagcca
     7981 atacgatcca ggaaattgaa ttgaagatca tgaattcgat ttttgccaaa aagcccaaaa
     8041 gagcggatgt ctattggttt ctccacctgc accgcaccac cagcccctat acccttgaat
     8101 acgatattca tgaactgctc gatgacaaag tgattaaaat caatatcaat atcggattca
     8161 ggatccaacc cctcacagaa ctctatttca aacaaattgt ccgtgacctg gtcaacaacc
     8221 aggaactcaa cctgcatatc cggcccgacg gatcgacccg ttacaatacc gatcccgatt
     8281 ttaaattcat tgtcattgag aaatacattt ccgtggaaaa cgtgttttct ttccgcgaag
     8341 ggatcctgct gaacggcttt ttcttcctta aaaacctcgg cctgaaggat gaaaacgctt
     8401 ttggcctgga taagagcgat gttgaaatcg aaagcatccc tctcgtcttc catccgattg
     8461 aaaacattaa tctccggcga ttggtcaagg aaaaagaacc ggtggccgaa agctgattga
     8521 attcgtttcc ggcgtatctt ccttttttcg caggcagctt ttgccaaaat atccccggcg
     8581 tattttccga tatcgccgat tccttttaaa ttcgtttgcc cgccctaatc caggttttcc
     8641 aaatttcaac gatcccagca tgaaatattt gtcttttttt attttttccc tggtcatgca
     8701 agcagcaggc tatgcggacg acgggtaccg cctttggttg aaatacgatc tgatcgaaga
     8761 ccctgcattg ttaaaaatgt accggtccgg gatccgttcc atccggttgc aggcggctga
     8821 aacagccctt acagcgagtg cggtccgtga actgaccacc ggccttatgg gattattggg
     8881 aaaggccata aactctacga acaagcctgc aagttcagct caaatcatcc tcagcctctc
     8941 gccaccggga ggagtgatca atcatgaaga gggataccga gtggtgaagg ataaaaataa
     9001 tatccggata gaggccctga ccgaccgggg cctgttgtat ggcagctttg cgttgctggc
     9061 ctaccttcaa caggcgaggc ccctccatgc ccttcatctg gaatcgagcc caaagatcaa
     9121 gttgcggctc ctcaatcact gggacaatcc caacgggacc attgagcgag gatacgccgg
     9181 ttcctcttta tggaaatggt acgacctgcc cgatcaaccg gatcccaggt acagggacta
     9241 tgcccgggcc aatgcatcca tcgggatcaa cggcaccgtg gtgaataatg tcaatgccag
     9301 tgcccgtttt ttgagcaggg aatacctggt caaggtcgcc gccctcgcct ccattttcag
     9361 gcaatacggc attaaaacct atttatccat ccggtttacg gcccctacct acctggacgg
     9421 attcaagacc gcggaccccc tggatccggc cgtgcgccaa tggtggaaag aaaaagccag
     9481 cgagatctac agccttattc ccgatttcgg aggcttcctg gtaaaagcca attccgaagg
     9541 ggaaccggga ccccaggatt ataaccgcac ccatgccgac ggagccaata tgcttgcgga
     9601 ggccctggcg acccatggcg gtatcgtgat gtggagggct tttgtttaca aggccaatcc
     9661 caataacgac cgcgcttcag aatcttataa tgaattcaaa ccgctcgatg gtcagttcct
     9721 ggataatgtg ctggtccagg tgaaaaacgg gcccatcgat ttccagcccc gggaaccttt
     9781 tcatcctttg tttggggcca tgccccgcac ccccttgatg atggaattcc agatcaccca
     9841 ggagtacctg ggatttgcca cccattgggt ctatctggcc aatatgttca aggaagtatt
     9901 ggacagcgat acctattcca aaggacctgg atctacagtc gcgaggatca tcgatggcac
     9961 agtggaccgg cacaagttga acggtatcgc cggagtggcc aacaccggtt ccgaccgcaa
    10021 ctggtgcggg catattggca accaggccaa ctggtatgcc ttcgggaggc tggcctggga
    10081 tcctgatctg cagaccggct ttattgccga agactggatt cgtatgacgc tcacccggaa
    10141 taccgctgat gttgacgtga tcaaaaagat catgatggac agccacgaag cctgtgtgga
    10201 ctacatgacc ccccttggac tgcatcatat catgggtgaa agcattcact tcggccccca
    10261 gccctggctg gcccggagcg gtcgcgcgga ctggacctcg acctactacc accgggccga
    10321 cagtatagga ctgggttttg accgcaccga aggcggcagc atagccatcg gcctttatgc
    10381 cccagaggtg caggcccaat ggaaggatcc cgaaaccatc cccctgaaat acctgctttg
    10441 gttccatcac gtgaattgga ataaaaagct gaatacgggc aatacgctgt gggaagaact
    10501 ctgcaaacga tattattcgg gag
//
LOCUS       DAOQXT010000137        10467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6816_length_10467_cov_6.028717, whole genome shotgun sequence.
ACCESSION   DAOQXT010000137 DAOQXT010000000
VERSION     DAOQXT010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6816_length_10467_cov_6.028717"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..360
                     /locus_tag="PKM27_17340"
                     /pseudo
     CDS             55..360
                     /locus_tag="PKM27_17340"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
     gene            418..1299
                     /locus_tag="PKM27_17345"
     CDS             418..1299
                     /locus_tag="PKM27_17345"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNR09089.1"
                     /translation="MNRPKKIRKPFLCTSVIVLTMMASTHSFAQTLQVMSFNIRYDNP
                     QDGENRWDNRKKNLASMLRFYEVDLCGMQEALIHQIRDLKAALPEYEYIGKGRDDGRE
                     AGEFSPIFYRKDRIRVLESGTFWLSPSPDTPSRGWDAMLPRVVTWAKIKTIKGNKTAY
                     VFNTHYDHMGEIARRESSNLLLRKIAEMAGKSRVVVTGDFNAIPSQDPIRILLDTSNP
                     GHLTDTESLTRQEHFGPYSTFNGFEAMEQEGRHIDYIFLKNFKKMQVLRHGTLSNTWS
                     GRFASDHHAVLAELSLK"
     gene            complement(1309..4062)
                     /locus_tag="PKM27_17350"
     CDS             complement(1309..4062)
                     /locus_tag="PKM27_17350"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNR09090.1"
                     /translation="MKHFHLFCLLVLGFELGAQGIPLPEHPRPDFERDRWVNLNGPWE
                     FEFDSLDKGTEEKWFEAKKFSVAITVPFPWGSALSGVKDEADLAWYRKTITVPSSWEG
                     NRTFLTIGASDWETQVWFDGKWIGSHRGGYMPFSFDLSPYVRYGQPQNLVIRVDDKRR
                     MFTLYGKQGYGNARGIWQTVYLESRGQDYVESIQCIPDLGRKGVTIKVFLPSEAKTSI
                     PMKVKVKTPQGDQVQNLQVGVGNISAQTFLSIPQPRLWTLRDPFLYDMEVQLGGDRVR
                     SYFGMRKISVTSLPGTAIPYIALNDEPVYLQLALDQSYHPEGYYTFPSDSFMRQEIER
                     SKTIGLNGIRTHIKPDLPRKLYWADKLGLLVMADLPNSWGEPDELMRQESESTLREMI
                     KRDFNHPSIFSWIVFNETWGLTTRMEKESKPVQVYLPNTQHWVASMYYLAKSLDPTRL
                     VEDNSICCGRGHTETDIYSWHEYLAGWEWEDHLKKISDNNYPGSAYHFEPGFQQQSQP
                     NINSECGNVWGYEGSTGDVDWTYDYHRMINTFRKYPKVAGWLYTEHHDVINEWNGYWR
                     FDRSLKFTGLEDLVPGMSLLDLHSKYYVSTGNEICKTAMGGELVEVPLYLSIYDGGPV
                     PGNRLTLEYSLDFTDAGGETSEILAGTMPIDARPWDQKALKSLQLQMPDYRGLGILRL
                     VLKTESGEILHRNFMHFEVQSGQDPAGKLIFSARPDAFTAQQWSSKFWKVLDGKKVNG
                     AGTGYFEYRIPVDKKSKFQGIRSGYLVMEVSAKQLFDKDQEGAFDQNQNYMLGSRVSP
                     HKNPNAYPMTDEEKFPSEVQVWINGRMALRSKLEDDPADHRGVLSWYHQLKDKKLREA
                     GSYGYLIRVPLKKAEWQAARSRGYLSVKLQSSGKGGLAVYGSQFGRYPVDVSLVLDKS
                     AER"
     gene            4384..4854
                     /gene="greA"
                     /locus_tag="PKM27_17355"
     CDS             4384..4854
                     /gene="greA"
                     /locus_tag="PKM27_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNR09091.1"
                     /translation="MELTLMTSEGFAKVKAELDELKGNGRREAARAIAEAREKGDLSE
                     NAEYHAAKDAQGMLEFKINELEKKLANVKLIDDSQIDTSKVGILSRVTIRNKKTGKDV
                     IYKLVSEAESDIKSGKISVNSPIGQGLLGKNVGEIASVATPNGTIEFEVIRISI"
     gene            4862..5257
                     /locus_tag="PKM27_17360"
     CDS             4862..5257
                     /locus_tag="PKM27_17360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNR09092.1"
                     /translation="MASIFTKIVRGEIPAHKIAEDDHFLAFLDIQPLAAGHTLVIPKK
                     ETDYLFDLEDRDLGAMMVFSKKIAKALRAAVPCLRIGVCVIGLEVPHAHIHLIPLNHI
                     GDINFERPKLHPGTEELHAMAENIRRQLA"
     gene            complement(5254..5841)
                     /gene="ruvC"
                     /locus_tag="PKM27_17365"
     CDS             complement(5254..5841)
                     /gene="ruvC"
                     /locus_tag="PKM27_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNR09093.1"
                     /translation="MPVKSFGHRILGVDPGTQFLGFALIEPLSDEIRILAMDTIRLKD
                     AGDHQDKLREIFLQLQELIEAYRPGQMAIEAPFYGKNVQAMLKLGRAQGVAIAAGITM
                     GLEIHEYSPKRIKQAVTGNGNAAKEQVAAMLSHILKKPVQADRFDATDALACAICHHQ
                     QGHSHAPPPGRGKKYNSWKGFLDENPGRAAKPHRA"
     gene            6111..6962
                     /locus_tag="PKM27_17370"
     CDS             6111..6962
                     /locus_tag="PKM27_17370"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNR09094.1"
                     /translation="MMPVLKYPLFILFSLLSAGRPDGTKPEARSALKEPDFIPVHDSG
                     TFMAGEELTYKIYYNWNFIWLAAGEVVFKVEDLGDKYKLSARGRTYSSYEWFFKADDY
                     FESIVRKSDLLPVSFIRDIKEGKYRYFEKIEFDQQGLQLKTWTGKSPETASESTHRVN
                     KNMHDVLSLVYSMRTHSFADTQEGSVLPLNIFLDKKAYDLKLVYYGTKENKKVHRLGR
                     YTSHHLSPEVIAGSVFKENDRMHIWASADKNQVPLLIESPVSVGSVKAVLSKYKGLKY
                     EFYQPLP"
     gene            6962..7561
                     /locus_tag="PKM27_17375"
     CDS             6962..7561
                     /locus_tag="PKM27_17375"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNR09095.1"
                     /translation="MLKLRYLLYLALLAVIAGGLWWLSSEWVARSRVHTTQNATILLE
                     RIQKVAKLTTVEGYFSELYENRSFQYFDIYPFQKKVLVRVKAKVSAGFDLEQLSLETD
                     QAGKTIWINALPEPTILSIDHDLDYYDISEGLFTSFSEADYNKILSEAKDLIRKKASE
                     SSLLAEASAQGKDFLDMVRLIAESSGWQLKIRSRPEMKQ"
     gene            complement(7568..9916)
                     /locus_tag="PKM27_17380"
     CDS             complement(7568..9916)
                     /locus_tag="PKM27_17380"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNR09096.1"
                     /translation="MITLVLQSSLCWLFFMLIYRFFLQKETFFRFNRYYLLGTLLLGL
                     WIPLVRLFWEQSGGSEIPVYLGFQGSAQVLDLHLAEAGNPDSPDWLGILVKVYWGGVL
                     ICFLNLMRELAGILNWIRKYPKQRYPHYTLVQTDFEHLPFSFLHWVFISDKVKFRQKE
                     LRDILFHETSHVSGWHSLDVLLAEGIAALAWFSPMVYWYKRSIRNTHEYLADALVTRS
                     ENKDHYGRLLIDHAQRRLQIALANHFIYSQLKNRIHMLMKSESKPIRAWKYALVLPIM
                     FFLGVLFAYKSEALPAGYDGPMGDRMFSWSDTIHTQVDEMPVFPGCAHLTDLKERSNC
                     SMQKLMDYLVKNVQYPLAAKAKNVQGKALVSFVVNTDGSISDIELKESPGSGTGEEAL
                     RVVRRMNQLQENWIPGKENHKTVRVRMTLPILFKLQDEKIRDYPEQALYFLNGDRASL
                     DEIKNIFPDQILSMNVVKGETALEKYGEEGRQGVIEILTKPKYVVNGVETTKEEADKI
                     PADQIESVNVVKYGKKEAGVVDIKTKKDYSIEKGQDGTMDAFPLHKIEKGNAREIFKT
                     VEDLPRFPGCEHLTDKAARDQCAFQLMLEYIYKNIKYPKTAKEQGIEGRVVASFVVEP
                     DGRLSDIKIVKEVGSGCGDEVLRVVKSMDSMDQKWIPGKQNGQAVPVQYNLPVEFKLS
                     KEPVPVKEPAAVTAAPGLRGNTEFKLIPNPARGEVEIRWDRRGQVRIEIFDLNGKSLH
                     QSNHPDFNGFHKIDVRRFSPGTYIARVIQGTESQEQKLLIGQ"
     gene            complement(9918..10286)
                     /locus_tag="PKM27_17385"
     CDS             complement(9918..10286)
                     /locus_tag="PKM27_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNR09097.1"
                     /translation="MRKTLTKAEEDIMQIIWSKGRVLVSDILDELGDPRPPHSTISSI
                     VRILEKKGFVGHKAYGRTYEYFALLPKEEYSKHTVRNLIEEYFGGSANSLVSFLVKEK
                     DLSLKELAELMEVLRKNKNK"
BASE COUNT         2594 a         2690 c         2536 g         2647 t
ORIGIN      
        1 ctcctcgctt gactttcaaa ccatagacgg aaaggtcatt ggttcacaga ccaccctgaa
       61 cctgcacgca tacggccccg cccctttctc ctcttgccgg cctacgatga atacctgatt
      121 gggtttactg accgcagctt cgccatccac ccggagttta tcccggtagt catgacttcc
      181 aacggtatcc tccaccccac cctggtatac cggggaaagg tacttggcct ctggaaacgg
      241 aaaccgggat cgaaggtcca ggaggtgcaa tccaactggt ttttcaaacc aaatcaatcg
      301 cagaggaact tgtttaagca gtccattggc ctataccaac ggttctacag gcgtcattga
      361 cgtttaagtt gacctaaccg gacaggatgt acacaccgcc tcattatatt tgatggtatg
      421 aatcgaccaa aaaagatccg gaaacccttt ctctgcacat ccgtgatcgt cctgacgatg
      481 atggcatcca ctcattcctt tgcccaaacc ctgcaggtca tgtctttcaa tatccggtac
      541 gacaatccgc aggatggaga aaaccgatgg gacaaccgga aaaaaaacct ggcttccatg
      601 ctccggtttt acgaagtgga cctttgtgga atgcaggaag ccctgattca tcagatccgt
      661 gatttgaagg cggctttgcc cgaatacgag tatatcggaa aaggaaggga cgatggccgc
      721 gaagccgggg agttttcacc catcttttac cgtaaagacc ggatccgggt gctggaaagc
      781 ggcacctttt ggctcagtcc gagccccgat acgcccagcc ggggatggga tgccatgctc
      841 ccccgcgtgg tgacctgggc taagataaaa accattaaag ggaataaaac cgcttatgtt
      901 ttcaacacgc attacgatca tatgggtgaa atcgcccggc gggaaagctc aaatttattg
      961 ctcagaaaaa tcgcagaaat ggccggtaaa tccagggtgg tagtgaccgg ggattttaac
     1021 gcaatcccca gccaggaccc catccggatc ctcctggaca cctcaaaccc cggccatctg
     1081 accgataccg aaagtctgac gcgccaggaa cattttggtc catacagcac attcaatggt
     1141 tttgaagcca tggaacagga aggccggcac atcgattaca tcttcctgaa aaactttaaa
     1201 aaaatgcagg tgctcaggca cggtaccctc agtaatacct ggtcaggaag gtttgcatca
     1261 gaccatcatg cagtgctggc agaacttagc ttaaaataaa aaaagcgatc agcgttcagc
     1321 ggatttatcc aaaaccaggc tgacatccac cgggtatctt ccgaactggg agccatatac
     1381 tgccaaacct ccttttcctg aagactgaag tttgacggaa agatagcccc tggacctggc
     1441 cgcctgccac tccgcctttt tgaggggtac ccggataagg tagccgtatg aacccgcttc
     1501 gcgcagtttt ttatccttca actggtggta ccaggacaaa actcccctgt gatcggcggg
     1561 gtcatcctcc agctttgacc tgagggccat cctcccattg atccatacct ggacttccga
     1621 aggaaatttt tcctcgtccg tcatcggata ggcattggga ttcttatgtg gggacaccct
     1681 ggaacccagc atatagtttt gattttgatc aaaggccccc tcctggtcct tatcaaacaa
     1741 ttgtttagct gatacttcca tcaccaggta gccggaccgt atgccctgga atttggattt
     1801 tttatccacc gggatccggt attcaaaata gccggtaccc gccccattca cttttttccc
     1861 atcgagcact ttccagaatt tggaggacca ttgctgtgcg gtgaaggcgt ccggacgggc
     1921 actgaagatg agtttaccgg cagggtcctg accggactgc acttcgaaat gcataaagtt
     1981 ccggtgaagg atttccccgg actctgtttt taaaaccagc cggagaatac ccagcccgcg
     2041 ataatccggc atttgcagct gcaaggattt taatgctttc tggtcccatg gcctggcatc
     2101 aataggcatg gtaccggcca ggatttctga tgtctcaccg cccgcatccg taaaatcgag
     2161 ggaatactcc agggtcagcc gattgcctgg aacggggccg ccatcataaa tggaaaggta
     2221 aaggggtacc tccaccagct ctccgcccat ggctgtttta caaatctcat ttccggtgga
     2281 gacataatac ttgctgtgca ggtccaggag gctcattccg ggtaccaggt cctccagccc
     2341 tgtgaatttc aggctccgat cgaacctcca gtaaccattc cactcgttga tcacgtcgtg
     2401 gtgctcggtg tacaaccagc cggcgacctt tggatatttt cgaaaggtgt tgatcatacg
     2461 gtggtaatca taagtccagt ccacatcccc ggtgctccct tcatatcccc agacgttgcc
     2521 gcattccgaa ttgatattgg gctgggattg ttgctggaaa ccgggttcaa aatgataggc
     2581 gctgcccgga taattattat cggaaatctt tttcagatgg tcttcccatt cccatcctgc
     2641 caggtattcg tgccagctgt atatatcggt ctccgtatgc ccccgcccgc agcaaatgga
     2701 attgtcttcg accaggcggg tgggatccag ggacttggcc aggtaataca tggaagccac
     2761 ccaatgctgg gtgttgggaa gataaacctg gacgggtttg ctttcttttt ccatccgcgt
     2821 ggtcaatccc caggtttcat taaaaacaat ccaggaaaag atggaaggat gattaaaatc
     2881 ccgtttgatc atttcgcgaa gggtgctttc ggattcctgt cgcatgagct catccggttc
     2941 cccccagctg ttcggcaggt cggccatgac cagcaggccg agtttgtccg cccaataaag
     3001 tttcctgggg aggtccggtt tgatatgggt ccggatgccg ttgaggccga tcgtcttgct
     3061 cctttcgatt tcctgccgca tgaaagaatc ggaaggaaaa gtataatacc cttccgggtg
     3121 ataggattgg tccagggcga gttgaagata gacgggttcg tcattcagcg cgatataagg
     3181 aatggccgta ccgggcaggg aggttacgga aatcttccgc atgccgaaat acgaccgcac
     3241 cctatccccg cccaattgca cttccatatc ataaaggaag gggtcccgga gggtccataa
     3301 gcgtggctga ggaatggaca aaaaagtttg tgcactgata tttcccacgc ctacctggag
     3361 gttttgcacc tggtctccct ggggggtttt gaccttcacc ttcataggga ttgaggtttt
     3421 cgcttccgag ggtagaaaaa ccttaatggt tacccccttc cggcccaggt ccgggatgca
     3481 ttggatggat tcgacgtaat cctggccccg gctttcaagg tagaccgtct gccagatccc
     3541 gcgtgcattg ccatatcctt gtttgccata cagggtaaac atccgccttt tatcatcgac
     3601 ccggataacc aggttctgcg gctggccata gcgtacatac gggctgaggt caaaggaaaa
     3661 aggcatgtaa cctccccggt ggcttccaat ccactttcca tcaaaccaaa cctgggtttc
     3721 ccagtcactg gctccgatcg tcaggaatgt cctgtttcct tcccaggagg agggtacggt
     3781 gatcgttttg cgataccagg ccaggtcggc ttcgtccttt acgccggaaa gggccgaacc
     3841 ccatggaaag ggtacggtga tcgccactga aaattttttg gcttcaaacc atttttcttc
     3901 cgtccccttg tccagggagt cgaattcaaa ttcccaagga ccgttgaggt tgacccaccg
     3961 gtccctttca aaatcgggtc tcggatgctc cggaagaggt atgccctggg cgccaagctc
     4021 aaaacccagg accagcaggc aaaacaaatg gaaatgcttc atacaggtat ttggcgttaa
     4081 agatatgcat ccgggcggtg tccgggaccc ctgcacctct acgggatcat tataaaattc
     4141 cggaacggga agccggattt gttttacact ccgggaatta aaatattttg tcaggagcga
     4201 aatattgctt taactttgaa aatactaacc gactaaagaa cgtttttgga attactgaaa
     4261 acgttctttg tatttttata aaatcgtgat ggagtacctg gcttatccaa attcaatctg
     4321 cctgactgct gcaggccggg gtacgctctg atttattcac caaatttaac ctcattaatt
     4381 agtatggaat tgacactcat gacatcagaa ggttttgcaa aagtgaaagc cgaactggac
     4441 gaattaaaag gcaacggaag gcgcgaagca gccagggcca ttgcagaagc ccgggaaaaa
     4501 ggagacctgt ccgaaaatgc cgaataccat gccgccaagg acgcgcaggg catgcttgaa
     4561 tttaaaatca atgaactgga aaaaaagctg gccaatgtaa aactgatcga tgattcccag
     4621 atcgatacca gtaaagtagg tattctttcc agggtgacga tccggaataa aaaaaccggc
     4681 aaggacgtga tctataaact ggtatctgaa gcggaatccg atatcaaatc cggaaaaata
     4741 agcgtcaatt ccccgatcgg tcagggactt ttaggaaaga atgtgggcga aatcgcgtct
     4801 gtggccactc cgaacggcac cattgaattt gaagtgatcc ggatttccat ttaaccttcc
     4861 tatggccagc atttttacaa aaatagtcag gggtgaaatt cccgcacata aaattgcgga
     4921 agacgatcat tttctggcat tcctggatat tcaaccgctc gccgcgggcc ataccctggt
     4981 catccctaaa aaggaaaccg actacctctt tgacctggaa gaccgggatc tgggagccat
     5041 gatggtcttt agtaaaaaaa tagccaaagc gctccgggcg gccgtaccct gccttagaat
     5101 cggggtttgc gtcatcggcc ttgaagtgcc gcatgcccac atccatctga tccctttaaa
     5161 ccacattggg gatattaatt ttgaacgtcc caaactccat cccggcacgg aagaactcca
     5221 tgcgatggcg gaaaacatac gaaggcaact ggcttaagcg cggtgaggtt tagcagccct
     5281 tcctggattt tcatccagaa accccttcca ggaattgtac ttctttcccc tgccgggtgg
     5341 aggggcatgg ctatggccct gctgatggtg acatatggcg caggccaggg catccgtagc
     5401 atcgaagcgg tcggcctgga ccggtttttt aaggatatgg gaaagcatgg ccgcgacctg
     5461 ctccttggcc gcattgccat tgccggtaac cgcttgcttg attcgcttgg gagaatattc
     5521 atggatctca aggcccatgg tgatgccggc tgcgatggcc accccttgtg ccctgcccag
     5581 ttttaacatg gcctgcacat tcttgccgta aaaaggggct tcgatcgcca tctgcccggg
     5641 ccggtaggcc tcaatgagtt cctgcaactg caggaagatc tcccgcaatt tgtcctggtg
     5701 atctcctgcg tcctttaacc ggatggtgtc catggcaagg atccggattt catcggacag
     5761 cggctcgatg agggcaaagc ccaaaaactg ggtgccgggg tccaccccga gaatccggtg
     5821 gccaaaggat ttgactggca tatgccgcgc aaattagaac atttttatat tagataattg
     5881 attaatgtga ccggatcctt gaacgaatgg tctgacctgg ggtcattccg gagtctatca
     5941 ggggttaacg aatcactaaa attaggtggc aggataaggg cttgaagccg gtgaaagtat
     6001 ggtatggtat tggatatgaa tcttttaaga aaaaatggcg ttcaaaggca agggccgccc
     6061 ggcatagaaa tcccggaatt tctatagcta attttacgtt atttaccttt atgatgcctg
     6121 tgctgaaata tccattgttc attctttttt ccctgctcag tgcgggacgg cccgacggaa
     6181 caaaaccgga ggcccggtca gccctgaagg aacctgattt tatacccgtt catgatagcg
     6241 gcaccttcat ggcgggggaa gaacttacct ataagattta ttacaactgg aattttatct
     6301 ggctcgccgc cggcgaagtc gtttttaaag tggaagacct gggggacaag tataaacttt
     6361 ctgcccgggg caggacctat tcctcctacg aatggttttt caaagcagat gactattttg
     6421 aatccatagt cagaaaatcc gatttattgc cggtcagttt cataagggat atcaaggaag
     6481 gcaaataccg gtattttgaa aaaattgaat ttgaccagca gggcctgcaa ctcaaaacct
     6541 ggaccgggaa gtccccggaa acggcctccg aatccactca ccgtgtcaat aaaaacatgc
     6601 atgacgtcct ctccctcgtt tattccatga ggacacattc cttcgctgac acccaggaag
     6661 gcagtgtgct gcccctcaat atctttctgg ataaaaaagc ctatgacctt aagctggtgt
     6721 attacggaac gaaagaaaac aaaaaagtac accgcctcgg cagatacacc tcgcaccacc
     6781 tcagcccgga ggtgattgcc ggttccgtgt ttaaggaaaa tgaccggatg cacatctggg
     6841 cgagtgccga caagaaccag gtgcccctcc tgattgaatc acccgtttca gtgggctctg
     6901 taaaagcggt gctttcgaaa tacaaggggc tcaaatacga gttttatcaa cctttgccct
     6961 gatgctgaaa ttaaggtacc tcctttacct ggccctcctc gctgtgatag ccgggggatt
     7021 gtggtggctg agttctgaat gggttgcaag gagccgggtc cataccaccc aaaacgcaac
     7081 catccttctc gaaaggattc aaaaagtggc caaactcacc actgtagagg gctatttttc
     7141 cgaattatat gaaaaccgga gctttcagta ttttgatatc tatcccttcc agaaaaaagt
     7201 gctggtcagg gtcaaggcaa aagtgtccgc cggctttgac ctggaacaat tgtcccttga
     7261 aaccgatcaa gccggaaaga ccatctggat caacgccctt cccgaaccga cgatcctctc
     7321 catcgatcac gacctggatt attacgatat ttctgaaggg ctcttcactt ccttttctga
     7381 agccgattac aataagatcc tgagcgaagc gaaagacctg atacgaaaaa aggcctcaga
     7441 aagcagtctg cttgctgagg cctccgcgca gggcaaagat ttcctggata tggtccgcct
     7501 gattgccgaa tcttcgggct ggcagttgaa aatcaggtca aggcccgaga tgaaacaatg
     7561 accggattta ttggccaatc aatagttttt gttcctggct ttccgtgccc tggatcaccc
     7621 gggcgatata ggtacccggg ctgaagcgcc ggacatcgat cttatgaaat ccattaaaat
     7681 cggggtggtt gctctgatgc aatgatttgc cgttgaggtc aaagatttca atccggactt
     7741 ggcctctccg gtcccaccgt atttccacct ctccccgggc cggattggga atcaacttaa
     7801 attctgtatt gccacgcagg ccaggcgccg cggtcacggc agccggttct ttgacaggta
     7861 caggctcctt ggataattta aattccaccg gcaggttgta ttggactgga acggcctgcc
     7921 cattttgttt tccgggaatc catttttgat ccatggaatc catggatttg accacgcgaa
     7981 gcacttcatc cccgcaaccg cttccgactt ccttcacgat tttgatgtcg gataaacggc
     8041 catccggctc cactacaaag gaggccacta ccctgccctc aatcccctgc tctttggctg
     8101 ttttgggata tttgatgttt ttatagatat attcgagcat aagctgaaaa gcgcactggt
     8161 cccttgcggc cttatccgtg aggtgttcgc aacctggaaa acggggcaag tcctccacgg
     8221 ttttgaagat ctcacgggca tttcccttct ctattttatg caggggaaaa gcatccatcg
     8281 tcccgtcttg ccctttctca atgctgtagt cctttttcgt cttgatgtcc accactccgg
     8341 cttctttttt gccgtatttg actacgttta cagactcgat ctggtcagca gggattttgt
     8401 ccgcttcttc cttggtggtt tccaccccat tcaccacata tttgggtttg gtcagaattt
     8461 caatgacgcc ttgcctgcct tcctccccgt atttttcgag ggcggtttct cctttaacca
     8521 cgttcatgga caggatttga tcggggaata tattttttat ttcatccagg cttgcccggt
     8581 ctccatttaa gaaataaagc gcttgttccg gatagtccct gattttttca tcctgcagct
     8641 taaacaagat gggcagggtc atcctgaccc ggactgtttt gtgattctcc ttaccgggaa
     8701 tccaattttc ctgtaactgg ttcatcctac gcaccacccg aagggcttct tccccggtgc
     8761 ctgaacccgg ggattctttt aattcgatat cggatatgct tccgtccgta ttgactacaa
     8821 aactgaccag ggccttgcct tgcacattct ttgctttggc tgccaggggg tactgtacat
     8881 ttttgaccag gtaatccatc agtttctgca tggaacaatt ggatcgttcc ttgaggtccg
     8941 tgaggtgcgc acaacccggg aagaccggca tttcatcgac ctgcgtgtgt atggtatccg
     9001 accaggaaaa catccggtcc cccatcggcc cgtcataacc ggccggaagc gcctccgact
     9061 tataggcaaa caagaccccg aggaagaaca tgatcggcag taccagggca tacttccagg
     9121 cccggatggg tttagattct gatttcatca gcatgtgaat tctgtttttt aattgtgaat
     9181 aaataaaatg attggcgagc gcaatctgaa gcctgcgctg ggcatggtcg atgagcaatc
     9241 tcccgtaatg gtccttgttt tctgaacggg tgaccagggc gtccgcaagg tattcatggg
     9301 tgtttcggat agaacgctta taccagtata ccatggggct aaaccaggca agggcggcaa
     9361 tcccttcggc cagcaagacg tccaggctgt gccagccgct gacatggctg gtttcatgaa
     9421 acaggatgtc cctgagttcc ttctgccgga atttaacctt gtcgctgata aacacccagt
     9481 gcaaaaagga aaaaggaagg tgttcaaaat cggtctgtac cagggtatag tgagggtatc
     9541 gttgtttggg atacttccga atccagttaa ggatgccggc caactccctc atcaggttta
     9601 aaaagcaaat cagcacacct ccccaataaa cttttaccaa gatgcccaac cagtccggag
     9661 aatccggatt gcctgcttcg gcgaggtgga ggtccaggac ctgggcgctg ccctgaaaac
     9721 ccaggtaaac cgggatttcc gaacctcccg actgttccca gaataatcgg acaaggggga
     9781 tccataaacc caataacaag gtccccagta gataatacct gttgaacctg aaaaaagtct
     9841 ctttctgtaa aaagaaccgg taaatgagca taaaaaacag ccagcaaagg ctgctctgga
     9901 gaaccagggt aatcatctta tttattttta tttttcctga gcacttccat gagttctgcc
     9961 agttctttca ggctcaggtc tttttccttc accagaaagg ataccaggct gttggccgag
    10021 ccgccgaaat actcctcgat caggttccgc acggtatgct tgctgtattc ctccttcggc
    10081 aacagggcaa aatattcata agtcctgcca taggccttgt gtccgacaaa gcccttcttc
    10141 tccaagatgc ggactatgga agaaatggtg ctatggggag gcctggggtc tcccaactca
    10201 tccaggatgt cgctgacaag cacccggccc ttcgaccaga tgatctgcat gatgtcctct
    10261 tccgctttgg taagtgtttt tcgcatcggt ttctttttgt aaaatcaaat atacaactaa
    10321 ttttttcgtt tgtcaactaa ttaataaatt atttgacgta tatatacgtt ataagaagtc
    10381 ggctatcagc tatcagctgt aactattgac tattgactac tgactactga ctactgacta
    10441 ctgactactg actactgact actgact
//
LOCUS       DAOQXT010000138        10447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_6842_length_10447_cov_6.442167, whole genome shotgun sequence.
ACCESSION   DAOQXT010000138 DAOQXT010000000
VERSION     DAOQXT010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6842_length_10447_cov_6.442167"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..2142)
                     /locus_tag="PKM27_17390"
     CDS             complement(115..2142)
                     /locus_tag="PKM27_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNR09098.1"
                     /translation="MNPIGVFMLFIGIAIVFIPLVSVERKGIVALATVIGIAMVSSIP
                     AIASLTGNPFEYVLQGSQITGRIPVRIDALSGWFILVLNFTFITGAWYGLQYMKAYRQ
                     QREMLSLHAISYILVFAGLIAISALQHSMAFLIAWEIMALSSFFLIIFERHRAEVIKA
                     GINYLIQSHVCIILLTLGFIWVAMNENSYDFNAIAQFTEKRPSSVGLALFLCLFSGFA
                     IKAGFVPFHTWLPYAHPAAPAHVSGVMSGVIIKIGIYGILRMLLLVRTDFLVLGYILL
                     FLSVLSGIYGVMLAIIQHNLKKLLAYHSIENIGIIGMAIGLGCIGIGKGNPGLVALGF
                     SGALLHTLNHSLFKSLLFYAAGHVYQATHTMDIEKLGGLVKKMPHTTLLFLVASLAIC
                     GLPPFNGFVSEFLIYTGMFRALPGSDFALLSLLVLGFFGLALIGGLAMFCFTKAVGTV
                     FLGSPRHPFAHDPGEAGWAKLMPMYVIVVLLLGIGLFPGVFVSLLSKPVELYSFISSG
                     NGLPGIFPAVETLSGIGWGAAIFLVLAGLIFWLRTRWVMNRVQTYDQTWACGYEGDTG
                     KMQYTASSFVRAYRKLVEPVLSIHKKKKEITGIFPVTAGQETHPGDKIEEWFIDLPVK
                     GLKKFFNRFIFLQNGNLQFYILYGVVFITLVLVLPLVIEYFSALIQFLNSI"
     gene            complement(2235..>2889)
                     /locus_tag="PKM27_17395"
     CDS             complement(2235..>2889)
                     /locus_tag="PKM27_17395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR09099.1"
                     /translation="SSLEEIKPIQKWFASDSDFNRLFPASMQILAIKHWTPVVVARKA
                     AGFLTNNRAVRVLDIGSGIGKFCLTAAYYYPHARFVGVEQREDLVAYAEKAKEVLQLE
                     NASFIHGNFTDLDFREYDHFYFYNSFYENLFGKDKIDDRVSFSSELYNQYSRSLNQQL
                     ERMPEGTRVAAFHSLEYEAPKGYRIVNSEFEDRLLYFIKQVSLRGVFRTERAEGTEG"
     assembly_gap    2890..2989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3211..7125
                     /locus_tag="PKM27_17400"
     CDS             3211..7125
                     /locus_tag="PKM27_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HNR09100.1"
                     /translation="MKSYLPTSLIYLGLTLVLLAACGPQKNKEAAQEGPVVLLISKAG
                     DSNSLSVQRKGRMEILLTQNALPDFRPYLHPIAAPDGKGRLTEYSPSHHKHQTGLYWG
                     FTRVNGKGLPMDTVHKYFYQKDKYPEMADGIGRDFFHHPEGDYWKRVSLDILTPEGQD
                     VSWQTVYDMLDAAGKPILRETQIWTMKEKEGKFILNLEWQGKAITDITIGEFDYGGLF
                     LRMPWKEGMKGQAVNAARQRNERAEGQRAMWVDVGMQVEGRDDLAHVAIFDHPENAGF
                     PQPWRVDGQLGIGPVRARLGDWKIKEGETEIIRHQIVAYTGVLNDVTMNALWDDYIGS
                     NSMYSTASLWNIAQEEARSAKFLNPEEAVAEMTLADGFKVNAWAGEPMVTQPIAFCWD
                     DRGRLWAAENRDYESRGAGFSNSGDSRIVILEDTDHDGAADSRKIFLEGIPFPSAIAV
                     GFDGLFLGAPPNLLFVPDKNQDDVGEQEDIEVLLTGWGIRDRHETINSFHWGPDGWLY
                     GLEGFATPSKIRKPVGKGKIYRHKEPFPEDLLEADGVDINGGVWRYHPLKKRFEAVAH
                     GFSNPWGIDYDRKGQLFITACVIPHLFHVIPGGIYHRQGGQHFNPYVYSDITTIVDHR
                     HRSAHGGARIYQSDAFPPVHQGRLFMANIHEHAVLSDVLENKGSGFVARHGEDFLMAN
                     NAQWIGFSMEIGPAGDMYVLDWHDADICGKEVLNKETGRIYRISPEKSLAENWEGRYA
                     DLKKMTDEQLVALQTNTSDWHARRARVLLQYRASKGSLASASVSRLRDLFRDNKNPDH
                     RLRAMWTLHITNNLSPDDLIKSLSDKDEYVRAWAVQMLCEDQKAPAKALERMIGMSKK
                     DPSPVVRLYLSAALQRVDADTRWRMAAALISRDRDVEDHNIPKMIWYGMEGLVAQSPE
                     RAIKMAAAGKLPLIAEFTARRLVDANALDLLVTELGKRPKTLKALLQGMLDGLEGRSD
                     AVAPANWEAVYDRLKTDKVVAAQAQAIAQLFGDTEAAQAFLATLKDPSAPMEARLEGI
                     KNLAARQNKELLPLLPSLLRELPLRVEVIRAIASYDHEPLGQSLLDGFAKYDLREKGE
                     ALQTLSSRTSYGRLLTQAIKTGKINKNEVPAYVARQLRRVVGNGFVEVWGPIDQLSGN
                     AALAYKRYQHLLQDQTLAGADLEHGKLLFQRTCGACHKLYGEGGILGPDLTGSNRSNL
                     NYLLGNLIEPSAEIQDDYRMVVITSRDGRTYSGNVTAENDRQLTLRVVGQDQLVINKS
                     DIQSRETAAVSLMPEGLLQALSDQEVVDLVGYLRAGQEEKLQ"
     gene            7233..7685
                     /locus_tag="PKM27_17405"
     CDS             7233..7685
                     /locus_tag="PKM27_17405"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR09101.1"
                     /translation="MSSIIYRNAEDEDISHLAEIRANRSATKEYWFDRIQKYSIGLVN
                     PQEALPPRVIYLALDGNKVVGFVAGHLTRRLNCDGELQWIDTVDTYRKKGIASNLIGV
                     LAKWFIGQQAYKICVDPGDEIARNFYLKNGANNLNEHWLYWPDIRTII"
     gene            8001..8798
                     /locus_tag="PKM27_17410"
     CDS             8001..8798
                     /locus_tag="PKM27_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR09102.1"
                     /translation="MDGTIVSFLSVGKGSGVIVLPGVLSMASDYSAFASALANNFAVH
                     TLERRGRGRSGPQGDGYSIQKEIDDVLALQRETGASLLVGHSYGGLIALEVARNKEAF
                     TKIAVYEPGVSIDGSMPVHWMSGYEKKLAENRNLDALVEFTLADAPARLARIPAWLMK
                     LILRIFFVRYPDSKKMLGLLRQNLHEWREIARLDGNYKDYREISAVVLLLYGGRSDSR
                     AVDLVIERLPAVIPHCETKVFPKLDHFGIERTAPGEVAKAIGDFFSK"
     gene            9066..9614
                     /locus_tag="PKM27_17415"
     CDS             9066..9614
                     /locus_tag="PKM27_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09103.1"
                     /translation="MINKMFFLFFLILFGWSNILMSQPKPFVPSDFKIPDTLETSYFR
                     IRMLTVNDVVKDYDAVMTSIDHLQKMYQSTSWPTKDLTFEQDLIDLGWHQKEFQLRRS
                     FAYTVVSLDESQVIGCLYINPTTKSDYDASITMWVRTSVLDKGFDSILFDSVKQWIAK
                     KWPFKKVAYPGREINWEAWRSL"
     gene            9910..>10447
                     /locus_tag="PKM27_17420"
     CDS             9910..>10447
                     /locus_tag="PKM27_17420"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR09104.1"
                     /translation="MKSITLKSTGETITFVKTGKDTEGAFTEVICSIPAGQEGPPPHI
                     HPLQDETFEVIEGKLELSAKGKKMVIEEGQSFIVTAKTAHTFSNPLDRETKFRAIYKP
                     ALDIDYLLMQGFDSLNSQANPNKPSFQMMVDFDYILKQIHGQYKFAGAPGFIFTIFAA
                     IGRLFTKPKVKSLKDHRAS"
BASE COUNT         2887 a         2467 c         2584 g         2409 t
ORIGIN      
        1 ttcaggggct ggaaaatgcc gggccctttc ctgccggagg ttatactttt ggtccggata
       61 acgattccgg taaaaatcag gctcatcagg atgatcaaaa taaaactgat catattatat
      121 tgaattcaag aattgaatca atgcgctgaa gtattctatg accaggggca gtaccaatac
      181 tagggtgata aatacgacgc cgtagagaat gtaaaactga agattcccgt tttgcaaaaa
      241 aataaaccgg ttaaagaatt tcttaagccc ttttaccggc aaatcaataa accattcctc
      301 tatcttatcc ccgggatggg tttcttgtcc tgcggtgacc ggaaaaatgc cggtgatttc
      361 cttttttttc ttatgaatgg ataaaacggg ttccaccaat ttgcggtagg cccgtacaaa
      421 cgaactggcc gtatattgca tcttgcccgt atcgccttca taaccgcagg cccaggtctg
      481 gtcataggtt tgtacccgat tcatcaccca tcgggtacgc aaccagaaaa tcaggccggc
      541 caggaccagg aaaatcgcgg cgccccaacc aatgccggac agggtttcaa cggctggaaa
      601 gatcccggga aggccattcc ctgatgaaat aaaactataa agttcaaccg gttttgatag
      661 taaagagaca aaaaccccgg ggaacaaacc tatgcccaac aacaaaacca cgatgacata
      721 catgggcatt agtttagccc atccggcctc acccggatca tgggcaaaag ggtgcctggg
      781 tgatcccagg aaaacggtgc ccaccgcctt cgtaaaacaa aacatcgcca atccgccgat
      841 cagcgccaat ccgaaaaaac ctaagaccag caaggagagc agtgcaaagt cggaccccgg
      901 caaagcgcgg aacataccgg tgtaaatcag gaattccgac acgaaaccat tgaatggcgg
      961 aagcccgcag atggcaaggg atgccaccag gaagagcagg gtcgtatgag gcattttttt
     1021 aaccaatccc cccagctttt caatatccat ggtatgggta gcctggtata catggccggc
     1081 cgcataaaac aacagagact taaaaagcga atggttgagg gtatgcaaca aagccccgga
     1141 aaagcccagt gccaccaaac cggggtttcc tttcccaatc ccaatacagc caagcccgat
     1201 ggccattccg ataattccga tattctcaat gctgtgatag gccagtaatt ttttcaggtt
     1261 atgctgaatg atcgccagca tcactccgta tatcccggat aggaccgata agaaaagaag
     1321 gatgtacccg agaaccagga aatcggtcct gaccagtaac agcatgcgta aaataccata
     1381 aatgcctatt ttgatgatca cgccggacat gaccccggat acatgggccg gagcagccgg
     1441 atgggcatag ggcagccagg tatgaaaggg cacaaagccc gctttaatgg caaagccgct
     1501 gaataaacat aaaaacaagg caagccccac ggaagacggc cgtttttcgg tgaattgggc
     1561 aatggcattg aagtcatagg aattttcatt catggccacc caaataaaac cgagggtcaa
     1621 caaaatgatg cagacatggg actgaatcag gtagttgata ccggctttga ttacctccgc
     1681 ccggtgtcgt tcaaaaatga tcagaaagaa ggaagaaagc gccatgattt cccaggcaat
     1741 caggaacgcc atcgaatgct gcagggcact gatggcgatc aggccggcga ataccaggat
     1801 ataactgatg gcatgaaggg ataacatttc cctttgctgc cggtaggcct tcatgtattg
     1861 caagccatac catgcgcccg tgataaaagt aaaattgagt accaggataa accagcccga
     1921 taaggcatcg attcggaccg ggatccggcc tgtgatctga gaaccctgga gtacatattc
     1981 aaaagggttg ccggtcagac tggctatggc gggaatgctg ctgaccatgg caattccaat
     2041 caccgtagcc agggcaacta ttcctttccg ttccaccgat accaaaggaa taaacacgat
     2101 ggctatgcca ataaatagca tgaacacacc tattgggttc atcctataaa tttaaaaatt
     2161 gttatgaaga agcttcgaat aataaaaagg aaacaataaa gtgcacattc atgcggaatg
     2221 gggatgaccc tcttctaccc ctccgtgccc tccgcgcgct ctgtgcgaaa cactccgcgc
     2281 aaacttacct gcttaataaa atacagcagc ctgtcctcaa actccgagtt gacgatccga
     2341 taccccttcg gcgcttcata ctccagactg tgaaaggccg ccacccgcgt cccttcgggc
     2401 atacgttcca gttgttggtt caggctgcgg ctgtattgat tataaagctc actggagaag
     2461 gagacgcggt cgtcaatttt gtccttgcca aacaggtttt cataaaagga gttgtagaaa
     2521 tagaaatggt cgtattccct gaagtccagg tccgtgaaat ttccgtggat aaaagaggcg
     2581 ttttccagtt gcaggacttc ttttgctttt tccgcatagg ccaccaggtc ctctctttgt
     2641 tccacgccaa caaaccgcgc atggggataa taataagcgg cggtcagaca aaacttgccg
     2701 atgccgctgc caatatccag gacccggacc gcccggttat tggttaaaaa accggcggct
     2761 ttgcgggcga cgacgacggg cgtccagtgt ttgatggcca ggatctgcat ggaagcggga
     2821 aacaggcggt tgaagtcgct gtcggaggca aaccatttct gtatgggttt tatttcttcc
     2881 agggaggacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cccacaacga
     3001 cacgaagacc acaaagaaaa tatagcacag aggtagggat actttaattt ttgtggttcg
     3061 aacgtgtttt tgtgggaatc cataccatct ccgtgccctc cgagtgctcc gtgcgaaccc
     3121 accccgtgcg aaccatctcc acgtcctccc gtaagaaaaa ctcaaataat cttaacttgt
     3181 tccaccatga tccccaccaa tttaatgaca atgaaatcat accttcccac ctccttgata
     3241 tatcttggtc taactctggt cctccttgcc gcctgcggcc ctcaaaagaa caaggaagcg
     3301 gcacaggaag gccctgtagt attattgatc tcaaaagccg gagattccaa ctccctttcg
     3361 gtacaacgca aaggaagaat ggaaattctg ctcactcaaa acgccctccc cgacttccgt
     3421 ccctatcttc accccattgc cgctccggac ggaaaaggcc ggttgacaga atacagcccc
     3481 tcccatcaca aacaccagac gggtctgtat tggggcttta cccgggtcaa cggcaagggc
     3541 cttcccatgg acaccgtcca taaatacttt taccagaaag acaaatatcc cgaaatggcg
     3601 gatgggatcg gccgggattt ttttcatcat cccgaagggg actattggaa aagggtatcc
     3661 ctggatattc tcacccctga agggcaggac gtttcctggc aaaccgttta cgatatgctt
     3721 gatgctgccg gcaagcccat cctccgggaa acccagatat ggaccatgaa agagaaggag
     3781 ggaaaattca tcctcaacct ggaatggcag ggcaaagcca tcaccgatat caccattggc
     3841 gaattcgact acgggggttt gtttttgcgc atgccctgga aagagggcat gaagggtcag
     3901 gccgtcaatg ccgcccggca gcgcaatgaa agggcggaag gccagcgcgc catgtgggtc
     3961 gatgtcggta tgcaggtgga gggacgggac gacctggcac atgtcgccat ctttgaccat
     4021 cctgagaatg cgggtttccc gcagccctgg cgggtggatg gccaattggg cattggtccg
     4081 gtgcgcgccc ggctggggga ttggaaaatt aaagagggag aaacggagat cattcgccat
     4141 caaatcgtgg cctataccgg cgtattgaac gatgtaacca tgaatgcctt atgggacgac
     4201 tacatcggca gcaattccat gtattccaca gcttctttgt ggaatattgc ccaggaagaa
     4261 gcccgttctg caaaattcct caatccggaa gaggctgtgg cggaaatgac cctggcggat
     4321 ggatttaaag tcaatgcctg ggccggcgaa cccatggtca cccaacccat cgctttttgc
     4381 tgggatgacc gcggacgctt gtgggcggcg gaaaaccggg actatgaatc caggggcgcc
     4441 ggtttttcca attccgggga cagcaggatc gtcattcttg aggatactga tcatgacggc
     4501 gcagccgata gccggaaaat attcctggag ggcattcctt ttccttccgc catagccgtt
     4561 ggctttgacg gcttgtttct gggcgctccc cccaacctgc tttttgtgcc ggacaaaaac
     4621 caggatgatg tgggggaaca ggaggatata gaggtcttgc tcaccggttg gggcatccgc
     4681 gaccggcatg agaccatcaa tagttttcac tgggggccgg atggctggtt gtatggcctg
     4741 gaaggatttg ccactccatc caaaatccgg aaaccggtgg gcaagggcaa aatttatagg
     4801 cacaaggagc ccttcccgga agacctgctg gaagcggacg gtgtggacat caacggggga
     4861 gtatggcgtt atcatcccct caaaaaaaga tttgaagccg tagcccatgg cttcagcaat
     4921 ccctggggta ttgattatga ccgcaagggg cagttgttta tcaccgcctg tgtgatccct
     4981 catttgtttc atgtgatccc cggaggaata tatcaccgcc agggcgggca gcacttcaat
     5041 ccttatgtgt acagcgatat caccaccatc gtggatcacc gtcaccggtc cgcccatgga
     5101 ggggcgagga tctatcaatc cgacgccttt cctccggtcc atcagggacg tttgtttatg
     5161 gccaatatcc atgagcacgc ggttttgtcg gatgtcctgg aaaacaaggg ttcgggtttt
     5221 gtggcccgtc atggggaaga ttttttaatg gccaacaatg cccaatggat cggcttcagt
     5281 atggaaatcg gtccggccgg ggatatgtat gtattagact ggcacgatgc cgacatctgc
     5341 ggcaaggaag tgctcaacaa ggaaaccggc aggatctacc ggatcagccc ggaaaaatcc
     5401 ttggctgaaa actgggaagg acggtacgcc gatcttaaaa aaatgacgga tgaacagttg
     5461 gttgccctgc aaacgaatac cagtgactgg catgcccggc gggcaagggt ccttctacaa
     5521 tatcgggctt ccaaaggttc acttgcttcc gcatcagtta gccggctaag ggatttattc
     5581 agggacaata aaaatccgga tcatcggctg agagccatgt ggactctgca catcaccaat
     5641 aatcttagtc cggatgattt gatcaaatcc ctgtcggata aggacgaata tgtccgggcc
     5701 tgggcggttc aaatgctctg tgaggaccag aaggcccctg ccaaagctct tgagcggatg
     5761 atcgggatga gcaaaaaaga tccttctccc gtggtccggc tttatctgtc cgcggccttg
     5821 caaagggtgg atgccgatac ccgctggcgg atggctgcgg ctctgatttc gcgggacagg
     5881 gatgtggagg atcacaatat ccctaaaatg atctggtatg gaatggaagg acttgtggca
     5941 caatccccgg agagggctat aaaaatggct gccgcaggca aactgcccct cattgctgaa
     6001 tttaccgccc ggcggctggt ggatgcaaat gccctggact tgctggtcac tgaacttgga
     6061 aaaagaccca aaaccctgaa ggccctgctc caggggatgc tggatgggct tgaaggcagg
     6121 tccgatgccg tcgcccccgc caattgggaa gcggtttatg acaggctgaa aacagacaaa
     6181 gtagttgccg cacaggccca ggcgatagcc caattattcg gggatacgga ggcggcgcag
     6241 gccttcctgg ctaccctgaa agatccgtct gctcccatgg aagcccgcct ggaagggatc
     6301 aaaaatctgg cggcgaggca aaacaaggaa ctgttgccac tgcttccctc cttgctccgg
     6361 gaacttcctt tgagggttga ggtcatccgg gccatcgctt cttacgatca cgagcctttg
     6421 ggccaatccc ttcttgacgg ttttgcaaaa tacgacctcc gggagaaagg cgaggctctt
     6481 caaaccctgt cttcccggac atcctatgga cgcctgctta cccaggcgat caagacggga
     6541 aagattaata aaaatgaagt tcccgcctat gtggcgcgtc agcttcgaag ggttgtgggc
     6601 aacggttttg tggaagtctg ggggcccatc gatcaacttt caggaaatgc cgctcttgcc
     6661 tacaagcgtt accagcattt gctccaagac cagaccctgg ccggcgccga cctggaacac
     6721 ggaaagcttt tgttccagcg cacttgcggc gcctgccata aactgtacgg ggaaggcggc
     6781 atcctgggcc ccgatctcac cgggtccaac cgttccaatc tcaattatct cctgggcaac
     6841 ctgatcgagc ccagcgcgga gatccaggac gattaccgca tggtggtcat caccagccgg
     6901 gatggccgca cctacagcgg caatgtcacc gcggaaaacg accggcagct taccctgcgg
     6961 gtggtcggcc aggatcaatt ggtgatcaat aaatcggata tccaatcgag ggagacggcg
     7021 gccgtgtctc taatgccgga gggattgctg caagcgctga gtgatcagga ggtggtggat
     7081 ttggtgggat atttgagggc ggggcaggaa gagaaattgc aatagaatat ttttagtatt
     7141 ttttggaaga tataaggaaa tatacgcaat aaacttgcgt atataactat attgggcgcc
     7201 atgctactaa catattaacc gtaaacagaa taatgtcgag tataatttat aggaatgcag
     7261 aggacgaaga tatttctcac ttggctgaga ttagggcaaa cagatctgca acaaaagaat
     7321 actggtttga cagaattcaa aaatattcta taggattggt aaatccgcag gaagctttgc
     7381 cgcctcgtgt tatttacttg gccctcgacg gcaacaaagt ggtcggcttt gtcgcaggcc
     7441 atctgacacg acgtctaaat tgcgacggag agcttcaatg gattgataca gtggacacat
     7501 atcgaaaaaa gggcatcgcc tccaacttaa taggagtact ggcaaaatgg tttattggac
     7561 aacaagcata taaaatttgt gtagaccctg gagatgaaat tgcccgaaac ttctatctta
     7621 aaaatggcgc aaataattta aacgaacatt ggctttactg gcctgacatt agaacaatca
     7681 tttaaggatt tatttcaaac ccgtaataaa acacggcgcc caacaaaggg attggcgtca
     7741 aggcggcttt acgaacatat tttcatcgat ataacattac tcagcaacat atcaatctga
     7801 cgaataatgc ccacctttag aatatatttt gaacttcata tctttgatca gcttcaatcc
     7861 cacttacgta acacggaata tcgccacgtc gccaagcccg gacgttgggc gtcatgctta
     7921 gacaacatct caacttccaa ctgaccatga caaagacaaa ctcgcgggac cgaaaaggtg
     7981 aatcacgtac tgtgatctcc atggatggta cgatcgtcag tttcctctct gtgggcaagg
     8041 gatcgggagt gatcgtgctc cccggtgttc tttcaatggc gagcgactat tctgcctttg
     8101 cttcggccct tgcaaacaac ttcgccgtcc ataccctgga gcgccgaggc cgaggcagga
     8161 gtggcccgca aggcgatggt tacagcattc aaaaggaaat tgatgacgtg ctcgcactgc
     8221 agcgggagac aggcgcaagc ttattggtag gccacagcta cggtggatta atcgccctgg
     8281 aggttgcccg aaacaaagag gctttcacaa agatcgccgt gtatgagcca ggtgtgtcaa
     8341 tcgacggctc aatgccggtt cactggatgt cgggttacga aaagaagctg gcggagaata
     8401 ggaacctcga cgccttagtc gagttcacgc tggcggatgc tccggctcgt ctggcgagga
     8461 ttccggcctg gcttatgaag ttgatactac gtattttctt cgttcgttac cccgattcca
     8521 aaaagatgct tgggctccta cggcagaatt tgcacgaatg gcgtgagatc gccagactcg
     8581 acggcaatta caaagattat cgtgaaatat ctgctgtcgt tctgctcctg tatggtggcc
     8641 gcagtgacag tagggcggtc gatttggtaa ttgagcggct tcctgcggtt atcccacact
     8701 gtgaaacgaa ggtattcccc aaactcgacc atttcggcat cgaacggaca gcccctggcg
     8761 aggttgctaa ggcgattgga gacttcttct ctaaataggg gaagatcaga gccaggactt
     8821 atattataat tgagattaac aaaaaaagaa aattaagtcg aacatttttc aattaatcca
     8881 ccaaacgaga atggatttat tggacaatgg attacgaggt gaaaaatgaa ataaattaag
     8941 agcacgaacg cccaacaaag agtatgtgtt catgtcgggc aaaagcccgt cacaccaaat
     9001 acacaaacca ttgggcacaa ttctttcata aaaccttcat atcaataatt cataaattac
     9061 cagggatgat caataaaatg ttttttttat tttttttaat tttgtttggt tggtcaaata
     9121 ttctcatgtc gcagccaaaa ccatttgttc cgtctgactt taaaatacct gatactttag
     9181 agacttcgta tttcagaata agaatgctca cagtaaatga tgttgtgaaa gattatgatg
     9241 cagtaatgac cagcattgat catttgcaga aaatgtatca atccacaagt tggccaacga
     9301 aagacttgac attcgaacaa gatttaattg atttaggatg gcatcagaaa gaatttcaat
     9361 tgagaaggtc attcgcatat actgtagtta gtttagatga aagtcaggtc atcgggtgtc
     9421 tttacattaa tccgacaaca aagagtgact acgacgcaag cataaccatg tgggtacgga
     9481 caagtgtgtt ggacaaggga tttgattcaa ttttgttcga ttctgtgaaa caatggattg
     9541 caaagaaatg gccattcaag aaagttgcat accccggaag agaaattaat tgggaagcat
     9601 ggagatctct gtaataatag ctttatcctt ttttaaaata aaaaaatgaa tatcaaaaat
     9661 gcgcataaca atggtttgcg cacgatccgg gctcgagggt ttgatttaat tcaacagctg
     9721 gcgtaaattg gctgaaaaga taggtagcca atttcaccag cctggctcat gccggacccg
     9781 gaatgcggca agcccggtac gttatgtgca tgcttgcccg ccaatgctaa cattaattta
     9841 tctggaatag acgacaaaac tggaagttaa aaacaatacg tattaagcac agaattaatt
     9901 aagggtaaaa tgaaatctat aacactaaaa tcaactggtg aaacaattac ctttgtaaag
     9961 acaggaaaag acacagaagg tgcttttact gaagttatct gcagtattcc agctggacaa
    10021 gagggtccac cgccacatat tcatccattg caagacgaaa cttttgaagt tattgaaggc
    10081 aaacttgaac tttcggctaa aggcaagaaa atggttattg aagaaggaca aagttttatt
    10141 gttaccgcaa agacggcaca caccttttca aatccgcttg acagagagac aaagtttagg
    10201 gcgatataca aacctgcact tgacattgac tatttgttga tgcaaggatt tgactcatta
    10261 aacagtcaag ctaatcccaa taaaccgagt tttcagatga tggttgattt tgactatata
    10321 ctcaaacaaa ttcacggaca atacaagttt gctggtgcac ctggttttat attcacgatt
    10381 tttgcagcta tcggaagatt atttaccaaa cctaaagtga agtcgcttaa agaccaccgt
    10441 gcttcgt
//
LOCUS       DAOQXT010000139        10189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_7047_length_10189_cov_5.011841, whole genome shotgun sequence.
ACCESSION   DAOQXT010000139 DAOQXT010000000
VERSION     DAOQXT010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7047_length_10189_cov_5.011841"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2387
                     /gene="pnp"
                     /locus_tag="PKM27_17425"
     CDS             <1..2387
                     /gene="pnp"
                     /locus_tag="PKM27_17425"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNR09105.1"
                     /translation="FPLSVDYAERFYAAGRIPGNFFRREGKLSDYEVLISRLVDRAIR
                     PLFPDEYMNETQVLLNLLSGDVESLPDGFAALAASTALVLSDIPWQGPISEVRIAKID
                     GQYVTNPSRSQLKQASLEFIIAATEKDLLMVEGEANECDESELIEAIQVAHETIKLQC
                     KAQLELAEKAGLAAIPKRTVTPLVIDEELDKLVHLATDEKLYFIARNPTDKKTRKDNL
                     DALAASLKEFIIASKSEEYYTENSKIISGLFEKNKKKIIREVVLSEGKRLDGRSTDQV
                     RPIWTEIDVLPAAHGSAIFNRGETQALGAVTLASKADEMLVDTAFEPHDEGFIFHYNF
                     PPFSVGEVKPQRGPGRREIGHANLAARSVRKVMPVDNPYTIRIVSDILESNGSTSMAS
                     VCVSSMALMDAGVKIKSPISGVAMGLIKEGDRAAILTDILGDEDALGDMDFKVTGTEK
                     GICACQMDMKIDGLAYDLLEKALAQAKAGRLHILGEMAQTVSEAREDYKPHAPRIVEI
                     RIPKSLIGAVIGPGGKVIQDIQATTGTVINVEEEGDEGIVSVASPNKESIDKAIARIQ
                     SIVFVPEVGETYEAKVVEILPFGVVVDFSGKQGLLHVSEISYTRIESVEEVFSIGDEL
                     KVKLMDIDARSGKYKLSRKALMDRPAGMPEDSGNDRGGDRGRGGRSGGGSYRDGGSGR
                     RSDDRGPRRDSRDRGGRDDRGGRDDRGGRDDRGGRDDRGGRDRDRDSRDGGRDRDRGR
                     RNDGPDRRPSSPSSRPEREADREGPAPNPDTFQQPPVDKPNYDAPRPEGLNWED"
     gene            2555..3421
                     /locus_tag="PKM27_17430"
     CDS             2555..3421
                     /locus_tag="PKM27_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNR09106.1"
                     /translation="MRQLKITNKITNRESVSLEKYLNDIAKLSMLTPEQEAEYARRIR
                     EGDEEALEIMCKSNLRFVVSVAKQYQNQGLSLSDLINEGNVGLIKAAKRFDETKGFKF
                     ISYAVWWIRQSILQAIVEYSRIVRMPVNKVGSYNRLNEAYITFVQEFEREPTPEELAE
                     ALGVAVKDVNHVLKSNMRHVSFDAPIGTDEDDISMIDIYMHETEDSPDLALMKESLKK
                     EVEYGMTVLAPREMEVLSAYFGLDGNKPLTLEEIGDMFGLTRERVRQVKERAIRRLRR
                     SMHQNSLKSYLG"
     gene            3431..4102
                     /locus_tag="PKM27_17435"
     CDS             3431..4102
                     /locus_tag="PKM27_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09107.1"
                     /translation="MIARAFIFLFITFLIFSFARVISILDKLFSGFRPVIQKLRRDLR
                     LLQPELKSWAADNLVKWDRQELELLSLRQHRQVKKSMRFKISRGVFQSIYHEPLLIYI
                     QKMYRPEEGVLLVRNSNHEFAYIIHHKETEVFIDEEYFGKLDSQGRLWYNQRNVIASI
                     DRGENVLLEIKVNNKLAGNLLNEAKKESRNPRAFQYVAELDATEEKVFLAIAFFEMIR
                     RSPKK"
     gene            4204..5796
                     /gene="bshC"
                     /locus_tag="PKM27_17440"
     CDS             4204..5796
                     /gene="bshC"
                     /locus_tag="PKM27_17440"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNR09108.1"
                     /translation="MTVDTLPLSSFPLLSKVDQDLNREDSPLDQFLKWKPQLDVFGEI
                     IQNRDREVNREKLHQVLSEQYQNLGIEIKIRDRIDALLDARTFTVCTAHQPCLFTGPA
                     FVLSKAISTIKLAQLIQEALPAYRIQPVFVIGAEDHDLEELNHCFLFGKKITWETRQS
                     GPLGRFDLERIEACISEAESLLVHTPFGPELIRLLREAHAGGRSFAEAFQYMMHVLLG
                     EHGLLVINMDDRRLKEEFKPWILDEVLHSSSKPLVTGTQARLMQLGYEASTHVRDINF
                     FYFDKGYRDRIERENGSFHISNQGLKLDEKSMAAEIEQHPEKFSPNVILRPIYQELIL
                     PNLAFVGGGGELAYWMERKSQFEALGVPYPALVRRDSFLVIQPEQKNLLDQFGLKVAD
                     LAERTDLLMNRMTEKLSSHELSLQEEILGITAWMDKIRSRAESVDPTLGPSIEAEKVK
                     MAKSIEHLEKKILKAEKSKLEVKLNKIRKLKEKLLPENGLQERRENFMTFYSEHGPAF
                     IRTLVHDFNPLDRQFKLIQIEA"
     gene            complement(5807..6658)
                     /gene="accD"
                     /locus_tag="PKM27_17445"
     CDS             complement(5807..6658)
                     /gene="accD"
                     /locus_tag="PKM27_17445"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNR09109.1"
                     /translation="MGWFKRLREGIQTTTKNKKEAPEGLWHKCEVCRHTSTSTELREN
                     FYKCVKCNHHVRIGSLEYFEIIFDQEKYTILLDNLEAVDILGFEDLKPYSRRLEEARQ
                     KSGYNSAVNVASGKVNKKNLVVACMDFSFIGGSLSSVEGEKICKAIDLCIESESPLLI
                     ISRSGGARMMESAFSLMQLAKTSAKLTTLADARLPYFSLMTDPTTGGVSASYAMLGDI
                     NMAEPKALIGFAGPRVIKEAIKKDLPEDFQSAEFLLEHGFLDFIIDRAKLRTRLDELL
                     TIFQLSQ"
     gene            6743..7465
                     /gene="dapB"
                     /locus_tag="PKM27_17450"
     CDS             6743..7465
                     /gene="dapB"
                     /locus_tag="PKM27_17450"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNR09110.1"
                     /translation="MKISLIGYGKMGKEIEQLCIKRNHNIYSIADPALNTGLDVLTGS
                     DLAIEFTEPRSAANNIRYCIDRGIPAISGTTGWQQDLEPVKSYCLEKKGCFFYSSNFS
                     IGVNLFWKVSRLLARVMDKQESYSLGLHEIHHDQKKDAPSGTAVTLAQHMLLESSRFR
                     DWRQVDAFTHALRESIPVSSERTGQVPGTHIVEYRSPYDRIVLTHEAFSRTGFVEGVV
                     AVAEWLQGKQGVFGMDDFFGKD"
     gene            complement(7502..7693)
                     /locus_tag="PKM27_17455"
     CDS             complement(7502..7693)
                     /locus_tag="PKM27_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09111.1"
                     /translation="MDKIFKNFFIYFILLTSVLIAGTSCGVGTGCPAQQGDSTKFDRK
                     GKLKGTGGKTRLFPKNMKK"
     gene            7874..9817
                     /gene="dnaG"
                     /locus_tag="PKM27_17460"
     CDS             7874..9817
                     /gene="dnaG"
                     /locus_tag="PKM27_17460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNR09112.1"
                     /translation="MILKKSVDEVLQAARVEEVVGDFVNLKRSGANLLGLCPFHHEKT
                     PSFSVSPSKNIYKCFGCGKAGGPVQFIMEHESCTFPEAIRYLAKRYNLTLEERQEPRT
                     EQEYQEEMKRESWGLVNDYAARLYHEALFSHASGKTIALPYFKERGILESTIKKFQLG
                     YAPEQRDYLVQHAREQGYNLQLLRDLGLTNKNDFDFFNHRVIFPIHNLAGKVIAFSGR
                     KIGKGDFGPKYMNSPETPLYSKSKVLYGMHLAKTGIRANDECILVEGYTDVLALHQAG
                     IQQVVASSGTSLTVEQIRLIKRFTTNIKVLYDGDEAGIKATLRALDLILDENMNVRLV
                     ILPPEDDPDSYLQKNGSESFKKYITEHSVDFVLFKASLIKKEYKNDPVKKSEGIADII
                     QTIARIPDAIKRSTYIKICAQELDVPENILISETNKKIRKEIFQRRTGTPGVDSDQAQ
                     EGNRTDFPAEPQRLPATEWDEHQERSLIRVLVLYGHLPMDDGSEKTMAQYINENMDEL
                     YEAFENKLYEKVLKDYMHQLSENRPVDTHYFSSHPDADIRNLAIEFCTSPYVYSHNWA
                     DRWEMELQTQKPPDQNYIKDCFQSILRFKLKKVQKMIADNKKLLESPDLDEMHQLICL
                     KAQHELLNHRNELADLLGMRVFS"
BASE COUNT         2744 a         2638 c         2536 g         2271 t
ORIGIN      
        1 actttccact ctcggtggat tatgccgaac gcttctatgc cgccggacgc atccccggca
       61 attttttccg ccgcgaaggc aaactttccg attacgaagt cctgatctcc aggctggtag
      121 accgggccat ccgccccctc ttccccgatg aatacatgaa tgaaacccag gtgctcctca
      181 acctgctttc aggggatgta gagtcccttc cggacggatt tgccgccctg gccgcctcca
      241 ccgccctcgt attgtcagac attccctggc agggaccgat ttcggaagtc cgcattgcaa
      301 aaatcgatgg acaatatgtc accaatcctt cccgcagcca actcaagcag gccagcctgg
      361 aatttatcat tgcggctacg gaaaaagacc tgctcatggt cgaaggagaa gccaatgaat
      421 gtgatgaatc cgaattgatc gaggccatcc aggtcgccca cgaaaccatt aagctccaat
      481 gcaaggcgca actggagctc gctgaaaaag ccggcctggc cgccatcccg aaacggaccg
      541 tgacccccct cgtcatcgat gaggaactgg acaaactcgt ccacctggcc accgacgaaa
      601 aactttattt cattgcccgt aatcccacgg ataaaaagac ccggaaagac aatttagacg
      661 cgctcgccgc ttctctgaaa gaatttatca ttgcctccaa atcggaggaa tattataccg
      721 aaaacagcaa gatcatttcg ggactgtttg aaaaaaataa aaagaaaatc atccgtgaag
      781 tcgtgctctc cgaaggcaaa aggctggatg gacgctccac cgatcaggtg agaccgatct
      841 ggaccgaaat cgatgtcctg cccgcggccc acggttccgc tatcttcaac cgcggggaga
      901 cccaggcgct gggagcagtg accctggctt ccaaggcgga tgaaatgctg gtggacaccg
      961 cttttgaacc ccacgatgaa ggatttatct tccattataa ttttccgcct ttttccgttg
     1021 gggaagtaaa accccagaga ggccccggcc ggcgtgaaat cggccatgcg aacctcgccg
     1081 cgagatcggt ccggaaagta atgcccgttg acaatcccta taccatccgg atcgtctccg
     1141 acatcctgga atccaacggg tccacttcca tggcttcggt ctgtgtttcc tccatggccc
     1201 tgatggacgc gggggtgaaa atcaaatccc cgatctccgg ggtagccatg ggcctcatca
     1261 aggaaggcga ccgggccgcc atactcaccg acatcctcgg ggatgaggac gccctgggcg
     1321 atatggattt taaggtcacc ggcaccgaaa aagggatctg tgcctgtcag atggacatga
     1381 aaatcgacgg cctggcttat gatctcctgg aaaaagcgct ggcccaggcc aaagccggac
     1441 gtttgcatat cctcggtgaa atggcccaaa ccgtcagtga ggcacgcgaa gactacaaac
     1501 cccatgctcc tcggatcgtg gaaatccgca ttccaaaaag cctgatcggc gcggtgatcg
     1561 gacctggcgg caaggtcatc caggatatcc aggccaccac gggtaccgtg atcaatgtgg
     1621 aagaggaagg cgatgaaggc attgtcagcg tagcgagccc caataaagaa tccattgata
     1681 aagccattgc gaggatccag tcgatcgttt tcgtacctga agtcggagag acctatgaag
     1741 ccaaagtggt ggaaatcctg cctttcggtg tggtcgtgga ctttagcggg aaacagggtc
     1801 tgctccatgt gtcggaaatc tcctataccc ggattgaaag tgtcgaggaa gtcttcagta
     1861 tcggggacga actcaaggtc aaactgatgg acatcgatgc ccgctcaggc aagtataaac
     1921 tttcccggaa agctttgatg gaccggcctg ccggcatgcc cgaagactcc ggaaacgacc
     1981 gcggcggcga ccgtggacgg ggtggaagat ccggaggcgg atcttacagg gacggcggct
     2041 caggacgccg gtccgatgac cggggtccgc gccgcgacag ccgtgaccgt ggaggacgcg
     2101 acgaccgcgg aggacgcgat gaccgaggag gacgcgacga ccgtggagga cgcgacgacc
     2161 gcggaggacg cgaccgggac cgggacagcc gtgatggcgg acgcgaccgc gaccggggcc
     2221 ggcgaaacga tggaccggac agaaggccgt ccagcccatc atcccgtccg gaaagggaag
     2281 cggaccggga aggacctgct cctaatccgg ataccttcca gcaaccccct gtggacaagc
     2341 ccaactacga cgcccccagg ccggaaggac tgaactggga ggattgatcc gggccgcgtt
     2401 ttagacaggc agctactgaa aataaggttt gtattagcct tattcataat aaaaagttaa
     2461 atcaacaaaa gggagcgagc gttttgcccc cttttgtgtt tattagagaa acttttaatc
     2521 ctataaaggt gtaaggtata tagaggaata aattatgcgc caattaaaaa tcacgaacaa
     2581 aatcaccaac agggaatctg tttccctaga gaaatatctg aacgatatcg ccaaactttc
     2641 catgctcact ccggagcagg aagcggagta tgccaggagg atccgggaag gagatgagga
     2701 agcccttgaa atcatgtgca agtccaacct tcgctttgtc gtatccgtag ccaaacaata
     2761 tcagaaccag ggactctccc tcagtgacct gatcaatgaa ggcaatgtag gcctgatcaa
     2821 ggctgccaaa cgtttcgacg aaaccaaggg gttcaaattt atttcctatg cggtgtggtg
     2881 gatccgccaa tccatcctcc aggccattgt cgaatacagc cggatcgtcc ggatgccggt
     2941 caataaagtc ggctcctaca acaggctcaa cgaagcttat atcacctttg tccaggaatt
     3001 cgaacgcgaa cctacgcccg aagaactggc tgaagccctg ggcgtcgccg tgaaggatgt
     3061 aaaccacgta ctcaagagca atatgcgcca cgtctccttc gacgccccca tcgggaccga
     3121 cgaggatgac atttccatga tcgacattta tatgcacgag acggaagaca gtcctgacct
     3181 ggccctcatg aaagaatcgc ttaagaagga agtggaatac ggcatgacgg tcctggcccc
     3241 aagggaaatg gaagtgctgt ccgcctattt tggactggat ggcaacaaac ccctgacgct
     3301 ggaggaaatc ggggacatgt tcgggctgac ccgggaaagg gtgagacagg taaaagaaag
     3361 ggccatccgg aggcttaggc gttctatgca ccagaattcc ctgaaatcgt acctgggata
     3421 aaaccggccg atgatagccc gggcttttat ttttttattt atcaccttcc ttatttttag
     3481 ttttgcccgt gtgatttcta tactggacaa actcttttca ggcttcaggc ccgtcatcca
     3541 aaaactgcgc agggacctgc gcctgctcca accggaatta aaatcctggg cggccgataa
     3601 cctggtcaaa tgggatcggc aggaactgga attgctcagc ctccgccagc accgccaggt
     3661 aaaaaagtcc atgaggttta aaatcagccg cggggtcttc cagtcgattt accacgaacc
     3721 cctgttgatc tatattcaga aaatgtaccg gcccgaagaa ggcgtgcttt tagtccgcaa
     3781 ttcaaatcat gaatttgcct acatcatcca ccacaaggag acggaagtgt ttattgatga
     3841 agaatatttt ggaaaactgg acagccaggg tcgcctttgg tacaatcagc gcaacgtgat
     3901 cgccagcatt gaccggggag aaaatgtgct tctcgaaata aaggtgaata ataaactggc
     3961 cggcaacctg ctcaacgaag caaaaaagga aagccgcaat cccagggctt tccagtatgt
     4021 tgccgaattg gacgcgacgg aagaaaaagt attccttgcc atcgcctttt ttgaaatgat
     4081 ccgcaggtcg cccaaaaaat aaacgaccgc tccccttcgt acgggttata aacgttacca
     4141 attccttaag tagggtaacg gaaaattgtt aacatgtatc tttgtgagct tcattaagta
     4201 tagatgaccg tagatacctt accgctttcg tccttcccat tattgagcaa ggtggatcaa
     4261 gacttaaacc gggaagacag cccgctggac cagtttttaa aatggaaacc gcaactggac
     4321 gtatttggag aaatcatcca aaaccgggac cgggaggtca accgtgaaaa gttgcaccag
     4381 gtcctgtccg agcagtacca aaatttgggt atcgagatca agatccggga ccggattgac
     4441 gccttgctgg atgcccgtac gtttacggtc tgcaccgctc accagccttg cctctttacc
     4501 ggccccgcct ttgtcctttc aaaggccatc agcaccatca aactggcaca actcatccag
     4561 gaggccctgc ccgcttaccg catccagcct gtttttgtga tcggcgccga ggaccatgac
     4621 ctggaagaat tgaaccattg ttttttattt ggtaaaaaga tcacctggga aaccaggcag
     4681 tcaggcccct tgggtcgatt tgacctggaa cgcatcgaag cctgcatttc tgaagcggaa
     4741 tccctgcttg tacatacccc tttcggccca gagttgatcc gattgctccg tgaagcgcat
     4801 gcaggcggcc gaagtttcgc ggaggctttc cagtacatga tgcatgtttt attgggtgaa
     4861 cacgggctcc tcgtgatcaa tatggatgac cggaggctca aggaagaatt caagccctgg
     4921 atcctggatg aagtcctcca ctcttcctcc aaacccctgg tcaccggcac acaggcccgg
     4981 ttgatgcaac tcggctacga agcatctacc catgtccggg acatcaattt tttctatttt
     5041 gacaaaggat accgggaccg gatcgaaagg gaaaacggat cctttcacat ttccaaccag
     5101 ggcctgaaac tggatgaaaa atccatggcc gccgaaattg agcagcatcc cgaaaaattc
     5161 agtcccaatg tcatcctgcg acccatatac caggaattga tcctgcccaa cctggccttt
     5221 gtgggcggcg gaggcgaact ggcctattgg atggagagga aatcccagtt cgaagccctg
     5281 ggcgtgccct accctgccct ggtgcgccgc gattcattcc tggtcatcca gccggagcaa
     5341 aaaaacctgc tggatcagtt tgggctgaaa gtcgccgacc tggctgaacg caccgacctg
     5401 ctgatgaacc ggatgacgga aaaactgagc agccatgagc ttagtttgca ggaagagatc
     5461 ctcggcatca ccgcctggat ggataaaatc agatcccggg cggaatccgt ggaccctacc
     5521 ctgggccctt cgattgaagc ggaaaaagtc aaaatggcca agagcattga acacctcgaa
     5581 aagaaaatcc ttaaagcgga aaaatcaaag ctggaagtca aactgaacaa gatccggaaa
     5641 ctgaaagaaa agttattgcc tgaaaacgga ctgcaggaaa gaagggaaaa ctttatgacc
     5701 ttctactccg aacatgggcc agctttcatt cgcaccctgg tgcatgattt taatccgctg
     5761 gaccgtcagt tcaaactgat ccagatcgaa gcctgactat tgttccttat tgggacaatt
     5821 gaaaaattgt gagcaactca tcgagccggg tcctgagttt ggcccggtct atgataaagt
     5881 ccaggaaacc gtgctcgagc aaaaactcag cggactggaa atcctccgga aggtcttttt
     5941 taatcgcttc tttgatcacc ctcgggccgg caaaaccgat aagagctttg ggttcggcca
     6001 tattgatatc tccaagcatg gcataggatg ctgaaacccc tcccgtagtg gggtccgtca
     6061 tcagggaaaa ataaggcagc cgcgcatcgg ccagggtagt cagtttggct gaagtcttgg
     6121 ccagttgcat cagggagaat gccgattcca tcatccgggc ccccccggac ctggatatga
     6181 tcagcagcgg ggactccgat tcaatgcaga ggtctatggc cttgcagatt ttttcgccct
     6241 ccacgctgga cagggaacct cctatgaagc tgaagtccat acaggctacc accaggtttt
     6301 ttttattcac ttttccactg gccacattga cggccgaatt ataacccgat ttctgcctgg
     6361 cttcttccag gcgcctggaa tatggtttta ggtcttcgaa tccaaggatg tccacggctt
     6421 ccaggttatc cagcagaatg gtatattttt cctggtcaaa gatgatttca aaatattcca
     6481 ggctaccgat ccggacatgg tgattgcact tgacgcattt gtaaaaattc tcccgaagct
     6541 cggttgaggt gctggtatgt cggcagactt cacatttatg ccaaagacct tcaggagctt
     6601 ccttcttgtt tttggtggtg gtctgtatac cttcccgtaa tcttttgaac caacccatgc
     6661 aaatttcctt tccgctgtcg cggaaggcgg caaatataac gaatccactt aatttagcgg
     6721 ttcaatccta aaattccgtc gaatgaagat tagcctgatt ggttatggca aaatggggaa
     6781 agaaatagaa cagttatgca taaaaagaaa tcataatata tattccattg ctgatcctgc
     6841 attgaataca ggactcgacg tattaacagg ctcagatctt gccattgaat ttacggaacc
     6901 caggtccgcc gccaataata tccggtactg catcgaccgg ggtatcccgg cgatcagcgg
     6961 gactaccggc tggcagcagg acctggaacc ggtgaaaagt tattgccttg agaaaaaagg
     7021 gtgctttttt tattcctcca acttctcgat cggggtcaac ctgttctgga aggttagccg
     7081 cctgttggcc cgggtcatgg ataaacagga gtcctattcc ctcggcctgc atgaaatcca
     7141 ccatgaccag aaaaaagacg ctcccagcgg taccgcggtg accctggccc agcacatgct
     7201 gctggaatct tccaggttcc gggattggcg gcaggtggat gcctttaccc atgctttgcg
     7261 ggagagcatc ccggtctctt cagaaagaac cggccaggtc ccggggaccc atatcgtcga
     7321 ataccgttcc ccttacgacc ggatcgtgct tacccatgag gccttttcaa gaacaggatt
     7381 tgtggaagga gtggttgccg tcgccgaatg gctccaggga aaacagggtg ttttcggaat
     7441 ggatgatttt ttcggaaagg attaaaatgt tccctcaggg gctgacgcga ttatcaggat
     7501 cttatttttt catgttttta ggaaacaatc tggttttccc acccgtgcct ttcagtttgc
     7561 ccttccggtc aaatttggtg gaatcgcctt gctgggcggg gcaaccggta cccacaccac
     7621 aggaggttcc ggctatcaat acgctggtta acaaaataaa ataaataaaa aaatttttaa
     7681 atatcttatc catgtgcccg aaatttgtaa taaaaaaaaa ccccactgac aaggactgtc
     7741 agtgaggtat gaacacacta tgagaacacc ctattcttat gtcaaatttg atggtgataa
     7801 cttatttaat tatcactcta caaatttaaa acatatttaa tataatttgc aaactattct
     7861 cagttttttt tcaatgatat taaaaaaatc tgtcgatgag gtattgcagg cagcccgggt
     7921 tgaggaggtg gtgggtgatt ttgtcaacct gaagcggagc ggggccaatt tgctgggcct
     7981 ttgccccttt caccatgaaa aaaccccctc tttttccgtc tctccatcca aaaacattta
     8041 caaatgtttt ggttgcggaa aagccggagg gccggttcaa tttatcatgg aacatgaatc
     8101 ctgcactttt ccggaagcca tccgttacct ggccaaacga tataacctga cgctggaaga
     8161 aaggcaggaa cccaggaccg aacaggaata ccaggaagaa atgaaaaggg aatcctgggg
     8221 cctggtcaat gattatgcgg cccggctgta ccatgaagcc ttattcagtc acgcctccgg
     8281 caagaccatc gccctcccct attttaagga acggggcatc ctggagagca ccataaaaaa
     8341 attccagttg ggttacgcgc cggaacagag ggattacctg gtccagcatg cccgggaaca
     8401 ggggtataac cttcaattgc tccgcgacct gggactgacc aataagaatg actttgattt
     8461 tttcaatcac cgggtcattt tccccatcca taacctcgcc ggcaaggtga tcgctttcag
     8521 cggcaggaaa atcgggaagg gggatttcgg tcccaagtat atgaacagcc cggaaacccc
     8581 gctgtattcc aaaagcaagg tgctctacgg catgcatctt gccaaaaccg gcatccgggc
     8641 gaatgatgaa tgtatcctgg tcgaaggtta taccgacgtc cttgccctcc accaggccgg
     8701 aatccagcag gtggtggcct cctccggcac ctccctcacc gtggaacaga tccggttgat
     8761 caaacgattt accaccaaca tcaaagtcct gtacgacggg gacgaggcgg gcataaaagc
     8821 caccctgagg gcccttgacc tgatcctgga tgaaaacatg aatgtccggc tggtgatcct
     8881 gcctcctgaa gacgatcccg attcctacct tcaaaaaaac gggtccgaat cgtttaaaaa
     8941 atacatcacc gaacattcgg tcgattttgt gctcttcaag gcctccctga tcaaaaagga
     9001 atataaaaac gatcctgtaa aaaaatcgga aggcattgcc gacatcatcc aaaccatagc
     9061 ccgcattccg gatgccatca aacgttcgac ctatattaaa atctgtgcac aggaactgga
     9121 cgtccccgaa aatatcctga tctcggaaac caataaaaaa atccgtaaag aaatctttca
     9181 gcgccggacc ggcacgcccg gagtggattc ggaccaggcc caggaaggca atcgcacaga
     9241 cttccccgcc gaaccccaac ggttgccggc caccgagtgg gacgaacacc aggaaaggtc
     9301 cctgatccgg gtcctggtgc tttatggcca tttgccgatg gacgatgggt ccgagaagac
     9361 catggcccag tacatcaacg aaaacatgga tgaactctat gaagcctttg agaataaact
     9421 ttatgaaaag gtattgaagg attacatgca ccagttgagt gaaaacaggc ctgtagacac
     9481 ccattatttc agcagccatc cggatgcaga catccgtaac ctggccattg aattctgcac
     9541 cagcccctat gtttacagtc acaactgggc cgaccgctgg gaaatggaat tacaaaccca
     9601 gaaacctccc gatcagaatt acatcaaaga ctgctttcaa agcatcctcc gatttaaact
     9661 gaaaaaagtg cagaaaatga tcgcggacaa taaaaaactg ctcgaatccc cggacttgga
     9721 tgaaatgcac cagttaatct gccttaaagc ccagcatgaa ctgctgaacc accgcaacga
     9781 actggctgac ctgctgggga tgagggtgtt ttcgtagggt tgaggattga gggttgaggg
     9841 gtgaggattg agggttgagg tacgaggaca gaggtacgag gtcagaggta cgaggtctta
     9901 ggaataaaaa tgtactcccg taacaattcc gcaattcaac aattcagcaa ttcaacaatt
     9961 ttcatttttc acaagtatga ataccaggcc gaatagtcaa tagtcaattg tcaatagtca
    10021 atagtcaatg gtcagtggtc agtggaattg gtgtgtaata aattgatcct aggaataaaa
    10081 atgaagtccc aaaacaaatc agcaattcaa caattcagca attcagcaat tagtatccct
    10141 tcacccttcg cccttcacct ttctcctttt aactcctaca gccgcggga
//
LOCUS       DAOQXT010000140        10185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_7049_length_10185_cov_6.762981, whole genome shotgun sequence.
ACCESSION   DAOQXT010000140 DAOQXT010000000
VERSION     DAOQXT010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7049_length_10185_cov_6.762981"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PKM27_17465"
     CDS             complement(<1..1117)
                     /locus_tag="PKM27_17465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR09113.1"
                     /translation="MKIAIISSRFPFPIDKGDKLRLFHQIKELSSRHQIILISLSDET
                     VGSRARKTLEDYCQKIYVFRLSRMQILFNLFKGLAKGLPAQVAYFFNEGIYGAIQKIL
                     RLEKPDHLYCQLIRTAEYGKSHPGFKTLDYMDCFHLSMLKRALHAGALMRRVWMEESR
                     RLKKYEGEIYPAFHNHTVISHPDREAMGLGMGQPLTVVPNGLDPGYFDQTEMRQKDID
                     LLFLGNLGYFSNRLAVRFLLFDLLPLIDDLKSLRITIAGARPGRRLRQWIRQSPRVSL
                     IADPPDTRNIYQRARLFVAPVFAGTGQQNKILEALASGCAVLCTPDVQKALDLPSPSL
                     IRVAVSAKEFASQIRSLLSAGVLPEGQISSARAYLQKH"
     gene            complement(1139..2350)
                     /locus_tag="PKM27_17470"
     CDS             complement(1139..2350)
                     /locus_tag="PKM27_17470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR09114.1"
                     /translation="MRILQLCKKFPYPLKDGESIAVTHLSKALYQMGCELTLLSMNTD
                     KHRFDTRHLPEDYVYFKEIHTVDVNISLNNWDAFVNLIKGSSYHVSRFISQEFNDKLV
                     DLLKSNTYDVIQLETLYLAPYIDTIQQHSNAIIALRAHNIEHEIWERIIEHHPNNLRK
                     WYLRHLTKQLKKYEVEQFEKYDLLIAFTDRDMGKFRKLGYLNGAITSPIGIELNQYQA
                     SFSKAGLFSVTFIGALDWMPNSEALTWFLNKIWNKLHLNHSDFVLHVAGRNCPEHFKN
                     LKLPGVIVHGEVEDSNSFISKHPVMVVPLLSGSGMRVKILEGMALGKVIVTTSLGLEG
                     IPARHHEEVLIADTPEEFIASFEYLYNHPEEMIRIGKQAVQFVSERYDTYKIASGLMH
                     KYQSLIEMGYK"
     gene            2584..3255
                     /locus_tag="PKM27_17475"
     CDS             2584..3255
                     /locus_tag="PKM27_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR09115.1"
                     /translation="MDHPIIKASQIHKSYDGRVVLKALDLEVGKGELVAIMGKSGSGK
                     STLLHILGTLDRPDSGEVSIRGEYPFRLSKNQLSDFRNRNIGFVFQFHHLLPEFDLLE
                     NVKMPGHIAKTDKKELHRKALELIDYFGLTGVMRQKPAELSGGEQQRAAICRALINQP
                     GLILADEPTGNLDQEASEELHALFGKLRKAFNQTFVIVTHQADLALQCDKTYILQDGS
                     LHLKT"
     gene            complement(3260..4246)
                     /locus_tag="PKM27_17480"
     CDS             complement(3260..4246)
                     /locus_tag="PKM27_17480"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNR09116.1"
                     /translation="MIRGVITIFFFLFYWTGFTQSVPKYALVEHFTNTWCPICAGRNP
                     SLFNALSNHTGRVHHVSYHPPYPYSGCPLYQFNKKDNQDRADFYQVFGTPTVVLNGGN
                     KMGGSPLLTETVLQAELQKTSPVSVRVSESGQGQNRSATVTVKSGVAINGDLRLMVLA
                     AEKNLQFTASNGELEHYNVMRKFLTPSTGQKLTIYSAGEQTFTFNFRDTAGWKAEEIY
                     VLAFVQNATTREVLNSGTRFDELSTPVHSAVQPVGIKIYPTLVEREFNVDHAFTGKTD
                     LKVYSIRGQLVLEQALAEAPYHAVSVSRLAPGAYWIKIMDGQAVYNARFIKY"
     gene            4668..5534
                     /locus_tag="PKM27_17485"
     CDS             4668..5534
                     /locus_tag="PKM27_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNR09117.1"
                     /translation="MQFPDLASGDFWISLLTLTFLEIVLGIDNIIFITIVASRLHKKD
                     QKKARSIGLVIAMLFRVVLLFGITAFMSMQQSLFSFDSSLASGAISIQSLILVAGGLF
                     LLYKAASEIFEKLEGIEHPGTHMPGKKVSLGNAIIQISLINLVFSFDSILTAVGLTKD
                     VTIMILAVVISILIMMAFAGPVGNFVNKHPSIQMLGLSFLILIGFMLIAEGAHEAHLV
                     VVGAEIGSIPRGYLYFAIAFSLFVEFLNIRMRKRREPVQLHSASREALKKGLMDHTSD
                     KPHADHEAGQNG"
     gene            5640..6707
                     /locus_tag="PKM27_17490"
     CDS             5640..6707
                     /locus_tag="PKM27_17490"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNR09118.1"
                     /translation="MRKIGPRQVFYFILLSAACKNPLDQLAFYEGKTMGTTYHVRIYL
                     EDKPEGLEAQLKKATDSLLYKINMGISTYENNSLVSRFNHSGQEDMGFTDTDSLGGSL
                     THFIQCLTMSRKIFDLSRGAFDPTIMPLVNYWGFGYTGRHEVSQPDTQKINELLLHSG
                     LDKIHFTRDSLAGTYLFHKEDTLLELDFGGIGQGYGADQIALLLDNLGYTRYLVELGG
                     ELVARGTKPDGSLWTVGLNTPEETAGTRDVFSQVRLTGKALTTSGNYRNFYKSGNQTF
                     SHTINTKTGFPERSVLLSVTLIGNDCTSIDGMATACMALGMGPCREVLGGQDMEAYFI
                     YRDGDHLRVDMTPGFKQYLIP"
     gene            6704..7528
                     /locus_tag="PKM27_17495"
     CDS             6704..7528
                     /locus_tag="PKM27_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR09119.1"
                     /translation="MNPFDFIRKDLVFLDIEATGPNFLKDRIIQLAMIKYQPGRENPL
                     EWNELINPGIPVSREAYEVHGIDAQALARKPLFGQLAENIHAFIGDADLVAYNTYKLD
                     IPILLEEFARAGMEWDLKNRKIIDAQRIFHKMEPRTLKAALKFYCNKDLSNAHDAMED
                     TRAIVEIFNGQIARYENVNMTDEEGKEYPAPVRRDIRALHDFTNDQNMVDITHRLKYN
                     AEGVMVFNFGKYIGQPVIEVFRKDRNFYHWIQNKEFSIQVKNILKNIFREHIDPRA"
     gene            7578..8339
                     /locus_tag="PKM27_17500"
     CDS             7578..8339
                     /locus_tag="PKM27_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09120.1"
                     /translation="MKINYDFKLSQWVLVFILAAVIGSCDKTESGPEGKMELVFTPRF
                     GAETFNTLQPYAYGDIQSIKLSKFDFYITGISLISASGNTLLEDAGLIDITGSSASSA
                     TLSLDGIKTGNYTGIKFSIGVIPELNAKDPKDFKSANPLSSTSHYWEAWDSYIFSKIE
                     GVLDTGGAKTYDLGFAIHTGTDECLATLTVTKNFTISEEQTTGLGLDLDVRRVFQENG
                     QFFNLPNSPLNHNPANIGVLKLFASNLAQAIQIKN"
     gene            8342..9457
                     /locus_tag="PKM27_17505"
     CDS             8342..9457
                     /locus_tag="PKM27_17505"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNR09121.1"
                     /translation="MLPWKRLLAGFSILILVFGLGCDFDPSEPEVGDLSQIPYSPERF
                     KVQVPAAFPELEVPANNPMTKAGVALGRKLFFDPILSRDSTQSCSSCHHPDKNFTDGL
                     PTSKGVDLINGRRSAMALIDIAFQTKGLFWDGRARLLEDQALLPVEDPVEMHFTWPGV
                     IERLKRHPEYPADFRKAFGITHKDSITKFLAAKAMAQFERTLVSSGATRYDLALTPGS
                     GVFFSESELRGYEMYFDLSGGRLPDAECFHCHNAPLFSSLDYFNNGIQRVVHGNQFED
                     NGLGAITGNAAQNGMFKAPSLRNIAQSAPYMHDGRIKTLEAVIEHYNSGGHPADNKNP
                     FVRKLGLTVQQKADLLAFLHTLTDTSFAQKPDYQSPF"
BASE COUNT         2554 a         2435 c         2597 g         2599 t
ORIGIN      
        1 aatgtttttg aaggtaggcc ctggcgctgc tgatctggcc ctcgggtaac acccccgccg
       61 ataacaggga cctgatctgt gaggcaaatt cttttgcgct cacggccacc cggatcaagc
      121 tgggcgaagg caggtccaag gccttctgaa cgtccggggt gcaaaggacg gcgcatccgg
      181 aagccagggc ttcgaggatt ttgttttgct ggccggtgcc cgcaaaaaca ggcgcgacaa
      241 agaggcgggc ccgctgatag atgtttcggg tatcgggggg atccgcgatc agggagaccc
      301 tgggactttg acggatccat tgccgcaacc ggcggccggg acgggcaccc gcgatcgtga
      361 tccggagcga tttcagatca tcgatcaggg ggaggaggtc gaataataaa aaccgtacgg
      421 ccaggcggtt ggaaaaataa ccgaggtttc caagaaagag gaggtcgata tctttttggc
      481 gcatttcggt ttgatcaaaa taaccggggt ccaatccatt gggaacgacc gtgagcggct
      541 ggcccatgcc gaggcccatg gcttcccggt cggggtgcga gatgacggta tggttgtgga
      601 aggcaggata gatctctcct tcgtattttt tcaaacgcct gctttcttcc atccataccc
      661 ggcgcatcag ggctccggca tggagcgccc ttttgagcat gctgaggtgg aaacaatcca
      721 tataatccag ggttttgaag cccgggtggc ttttcccata ttccgcggtc cggatgagct
      781 ggcaataaag atggtccggc ttttccagcc ggaggatttt ttggatggct ccatagatcc
      841 cttcattaaa aaaataagcg acctgggccg gcagtccctt tgccagccct ttgaaaaggt
      901 tgaagaggat ctgcatccgg ctcagccgga atacataaat tttctggcag tagtcctcca
      961 gggtttttct ggcccgggaa cccaccgttt catcggacag gctgatcagg atgatttgat
     1021 ggcgggaaga caactccttg atctggtgga acaatctgag tttgtccccc ttgtcgatcg
     1081 gaaatggaaa tcttgaactg atgatcgcaa tcttcaccgt gcagggaatg cagaggccct
     1141 atttgtaacc catctcgatg agggactggt atttgtgcat caggccggag gcgattttat
     1201 aggtatcgta cctttccgaa acaaattgca cggcctgctt cccgatccgg atcatttctt
     1261 ccgggtgatt gtacagatat tcaaaactgg cgataaattc ctcaggggta tccgcaatga
     1321 gcacctcttc gtgatgccgg gcaggaatgc cctccagtcc gagggaagtg gttacgatca
     1381 ccttgcccag ggccatccct tccaggattt taacccgcat gccgctcccg gaaagcaagg
     1441 ggaccaccat gaccgggtgt ttgctgatga aggaattgga atcttcgact tcgccatgaa
     1501 cgatcacccc gggcaatttc aggtttttaa aatgttccgg gcaattgcgg ccggctacat
     1561 ggagtacaaa gtcggaatgg tttaaatgaa gcttgttcca gattttattg agaaaccagg
     1621 tcagcgcttc ggaattgggc atccagtcga gggctccaat aaaggtgacc gaaaacaaac
     1681 ccgctttgga aaaggaagcc tggtattgat tcaattcgat cccgatgggt gaggtgatgg
     1741 ccccgttgag atatcccagt tttctgaact tgcccatgtc gcggtccgtg aaggcgatga
     1801 ggaggtcgta tttctcgaat tgttcgacct cgtatttttt aagctgtttg gtcaaatgcc
     1861 gcaggtacca cttccggagg ttgttgggat ggtgttcgat aatcctttcc cagatttcat
     1921 gttcgatatt gtgcgcccgg agcgcaatga tggcgttgga atgctgctgg atcgtatcga
     1981 tatagggagc caggtagagt gtttccagct ggatcacatc gtaggtgttg gatttcagca
     2041 agtccaccag tttgtcattg aattcctgcg aaatgaaccg cgaaacgtgg taagaacttc
     2101 ctttgatgag gttgacgaag gcatcccagt tgttgagcga gatgttgacg tctactgtat
     2161 ggatttcctt aaaatagaca taatcctcgg ggagatggcg ggtgtcgaaa cggtgtttgt
     2221 ccgtattcat ggaaagcagg gtcagctcac aacccatctg gtataaagcc ttggagagat
     2281 gggtgactgc gatggattct ccgtctttaa gagggtaggg gaatttttta cagagctgta
     2341 gtatgcgcat aaatcagttt cgcgaccccg caaatatggt taaaataatc gaattggttg
     2401 tttctttcat ttgccctcgt ttgggattcc ggcaactccc aaaacaggga cagcggcaat
     2461 ccaaagccaa cttatgttag ttaaaacgta aagccccgcc ttatttatta gctgaaggcc
     2521 taaaaaatat ggatttgggc caattatttt tcgcggcgga taaaaaagcg gaaatttgcc
     2581 gcaatggacc atcccatcat caaagccagt cagattcata aatcctatga cggaagggtg
     2641 gttctgaaag ccctggacct ggaggtggga aagggggaat tggtggccat catgggcaaa
     2701 tccggatcgg ggaaaagcac cctcttgcat atcctgggga ccctggaccg tccggatagc
     2761 ggcgaggtgt cgatccgggg cgaatatcct ttccgcttgt ccaaaaacca attgtccgat
     2821 ttccgcaacc gaaatattgg ttttgtgttt cagtttcatc atttgttgcc cgagtttgac
     2881 ctgctcgaaa atgttaagat gccgggtcat attgcaaaaa cggataaaaa ggaacttcac
     2941 cggaaagcgc ttgaactgat tgactatttt ggactgacgg gggtgatgcg gcagaaaccg
     3001 gctgaacttt cgggagggga acaacagaga gcggccattt gccgggccct gatcaatcag
     3061 ccgggcctca tcctggccga tgaacctaca ggcaacctgg accaggaggc ttcggaagaa
     3121 ctgcacgccc tgtttggaaa attaagaaag gccttcaacc agacctttgt gatcgtaacc
     3181 catcaggccg acctggccct ccaatgcgac aaaacctata tcctgcagga cggcagcctg
     3241 cacttaaaaa cctgagggtt taatatttga taaaacgggc attgtagacc gcctgcccgt
     3301 ccatgatttt tatccaataa gcgccgggag ccaggcggga tacagagacc gcatgataag
     3361 gcgcttcagc aagggcttgc tccaggacca gttgccccct gatactatat actttcaggt
     3421 ccgtttttcc tgtaaaggca tgatccacgt taaattcccg ttcgaccagg gtggggtaaa
     3481 ttttaattcc tacgggctgc acagcagaat gcaccggggt cgataattca tcaaatcgag
     3541 tcccggaatt taatacttcc cgggtcgtgg cgttttgcac aaaggccaaa acatagatct
     3601 cttcggcttt ccagcccgcc gtgtctctga aattgaaggt aaacgtttgt tctccggcgg
     3661 agtaaatggt cagtttttgg cccgtcgatg gagtgaggaa ctttcgcatg acgttgtaat
     3721 gctccagttc tccgttggag gccgtgaatt gcaggttttt ttcggcggca agcaccatca
     3781 gcctgaggtc accgttgatc gccacgcccg atttgaccgt gaccgtggcc gagcggtttt
     3841 gtccctggcc gctttcgctg accctgaccg aaaccggact ggtcttttgc aactcggcct
     3901 gaagtacggt ttcggtgaga agaggggatc ctcccatttt attgcccccg ttcaagacta
     3961 cggttggagt accgaagacc tggtaaaaat cggcccggtc ctggttgtct tttttgttga
     4021 actgataaag gggacagccg ctgtatggat aaggcggatg ataggagaca tgatgcacgc
     4081 gcccggtatg gtttgacagc gcattgaaca gggatggatt gcggcccgcg cagatcgggc
     4141 accaggtatt ggtaaaatgt tccaccagtg cgtatttcgg tacggactgg gtgaaaccgg
     4201 tccagtaaaa aaggaaaaaa aatatcgtga taacccctct gatcatgaat ttgaatatgg
     4261 atggtaaaga taagaacgta tggattccac gcttggttta ccggtgctat aatttaagaa
     4321 tacaggaagt gaaaccaagc cgaagctaag cttcttgcaa acaaatcgtt tgggaggagc
     4381 ttggctccgg ccacaccccg caagtttggg aggagcttgg ctccggcccc ttgaaaaact
     4441 gtaaatcatc attattcgta ccgggccgaa gctaagcttc gtccgaacag attgtaccag
     4501 gccgaagcta agcttcgtcc gaacgggtgc caaagctaag cttcgtccga acgggtgccg
     4561 aagctaagct tcgtccaaac acagtggttt gcttgcacca actttttatc cggtcgcgga
     4621 tgaacggcaa tcaaatctaa ttaattttga ccctttaaat attctgcatg caattccccg
     4681 atttggcctc cggagatttc tggatcagtc tgctcaccct caccttcctt gaaattgtcc
     4741 tcggcataga caatatcatt ttcatcacca tcgtagcatc caggctccat aaaaaggacc
     4801 agaaaaaggc caggagcatc ggcttggtga tcgccatgtt attcagagtg gtcctgcttt
     4861 tcgggatcac cgctttcatg tccatgcagc aaagcttgtt ttcatttgat tccagccttg
     4921 cttccggggc catctcgatc cagagcctga tcctggtggc ggggggattg tttctgcttt
     4981 acaaggccgc ttctgaaatt tttgaaaaac tggagggcat tgaacacccc ggcacccata
     5041 tgcccgggaa aaaagtaagc cttggcaatg ccatcatcca gatttccctc atcaacctgg
     5101 ttttctcttt tgactccatc ctcactgccg taggtttgac caaggatgtc accatcatga
     5161 tcctggccgt ggtgatttcg atcctgatta tgatggcctt cgctggcccg gtgggcaatt
     5221 ttgtcaataa gcacccaagc atccagatgc tcggtttgtc tttcctgatc cttatcggtt
     5281 ttatgctgat cgcggaaggc gctcatgagg cccacctggt ggtggtcggt gcggaaatag
     5341 ggtctatccc ccgcggttac ctgtattttg caatcgcctt ttcccttttc gtggaatttc
     5401 tcaacatccg gatgaggaaa cgcagggaac cggttcaatt gcacagtgca agccgtgaag
     5461 ccttaaagaa agggctgatg gaccatacca gcgataaacc ccatgccgac catgaagcag
     5521 gacaaaacgg ctaatcccga accgggatcc ccgatttcca ctccccggcc gggtatatta
     5581 ttattaccgg tatactgccc ggattcattt tgcaggggga ttaaaagact caaagtcata
     5641 tgaggaaaat tggtccgaga caggtatttt attttatcct gctgtccgca gcctgtaaaa
     5701 accccctgga ccagttggcc ttttatgaag gaaaaaccat gggaaccacc taccatgtga
     5761 ggatctacct ggaagataag cccgaagggc tggaggcgca actcaaaaaa gcgaccgatt
     5821 ccctcttgta taagataaat atgggtattt ccacgtatga gaataactcc ctggtttccc
     5881 gcttcaatca tagcggtcag gaggatatgg ggtttacgga tacggattca ttgggcggct
     5941 ccctcactca ttttatccaa tgcctgacca tgtcccggaa aatctttgat ctcagccggg
     6001 gggcttttga tccgaccatt atgcccctgg tcaattattg gggatttgga tataccggca
     6061 ggcacgaagt cagccagccg gatacccaaa aaatcaatga attgttgctt cactccggcc
     6121 tggacaaaat ccatttcacc agggattcgc tggccgggac ttatcttttc cataaagagg
     6181 atactctttt ggaactggat ttcggaggga tcggtcaggg ttacggagcc gaccagatcg
     6241 ctttactgct ggataacctg ggatataccc gctacctggt ggagctgggc ggggaactgg
     6301 tagcccgcgg tacgaaaccc gacggcagcc tttggaccgt tgggctgaat accccggaag
     6361 aaaccgccgg cacgcgagat gttttttccc aggtgaggct taccggtaaa gcgctgacca
     6421 cttccggcaa ttaccggaat ttttacaagt ccggaaatca gaccttctcg cataccatca
     6481 ataccaaaac gggttttccg gagcgttccg ttctgctcag cgtgaccctc attggcaatg
     6541 attgcacttc catcgatgga atggcaaccg cctgcatggc tttgggaatg gggccctgcc
     6601 gggaagtcct gggcgggcag gacatggaag cttattttat ttaccgggac ggagaccacc
     6661 tgcgggtaga tatgactccc gggtttaaac aatacctgat accatgaacc cctttgattt
     6721 tatccgcaaa gacctggtct tcctggatat cgaggccacc gggccaaatt ttctcaaaga
     6781 ccggatcatc cagctggcga tgatcaaata ccaaccggga cgggaaaatc ccctggaatg
     6841 gaatgaactg atcaatccgg ggatcccggt ttcccgggaa gcgtatgagg tccatgggat
     6901 cgatgcccag gcgcttgcac gcaaaccctt gtttggccag ttggctgaaa atatccatgc
     6961 cttcatcggg gacgccgatc tggtggctta caatacctat aaactggata tccccatact
     7021 cctcgaagaa ttcgcccggg cggggatgga atgggacctc aaaaacagaa agatcatcga
     7081 cgcccagcgg atctttcaca aaatggagcc ccgcaccctg aaagccgcgc ttaaatttta
     7141 ttgcaataag gacttgtcca acgcgcacga tgcaatggaa gacacccggg ccattgtgga
     7201 gatttttaac ggtcagatcg cccgttatga aaacgtcaat atgaccgacg aggagggaaa
     7261 ggaatatccg gctcctgtgc gcagggatat ccgggctctg catgatttta ccaatgatca
     7321 gaatatggtg gacatcactc accggctaaa atacaatgcg gagggggtga tggtctttaa
     7381 ttttggcaaa tacattggcc agcccgtcat cgaggtcttt cggaaagacc gcaattttta
     7441 tcactggata cagaacaagg aattctccat acaggttaaa aatatattaa agaacatatt
     7501 ccgggaacat attgatcccc gcgcttaagc ccccattccc tgcttattta ccttaacttt
     7561 gtaatataaa ctatattatg aagataaatt atgattttaa actatcccaa tgggtcctgg
     7621 tttttatcct cgcggcggtg atagggtctt gcgataagac agagtccggt ccggaaggca
     7681 aaatggaatt ggtgtttact ccccgtttcg gagcggagac attcaatacc ctgcagccct
     7741 acgcttatgg ggatattcaa tccatcaaac tcagcaaatt tgatttttac atcaccggga
     7801 taagcctgat ttctgcctcc ggaaacaccc tactggaaga tgcgggactg atcgacatca
     7861 ccggttcgag tgccagttcg gctaccctta gcctcgatgg gattaagacg gggaattaca
     7921 ctggcatcaa attcagcatc ggggttatcc ccgaattgaa cgccaaggat ccaaaggatt
     7981 ttaaaagcgc caatccgctt tcctccacca gtcattactg ggaagcttgg gattcttata
     8041 tattttcaaa gatcgaagga gtgctcgata ccgggggtgc aaagacctat gacctgggtt
     8101 ttgccattca taccggcacg gatgaatgcc tggcgaccct gacggtgact aaaaatttca
     8161 ccatttcaga agagcagacg accgggctcg gcctggacct tgacgtccgg cgggttttcc
     8221 aggaaaacgg acaatttttt aacctgccaa acagcccgct caatcacaac ccggccaata
     8281 tcggggtgct taaacttttt gcctccaatc ttgcccaggc catacaaatc aagaactgaa
     8341 catgttgccc tggaaacgac tgctggccgg tttttccatc ctcattctgg tttttggcct
     8401 tggatgtgat tttgatccga gtgaaccgga agtaggcgac ctgagtcaga tcccgtattc
     8461 cccggagagg ttcaaagtgc aggtccctgc cgccttccct gaactcgaag tgccggcgaa
     8521 taatcctatg acgaaagcgg gtgtggccct gggtagaaaa ttattcttcg accccattct
     8581 ctcgcgggac tccactcaat cctgttcgag ttgccatcat ccggataaga attttaccga
     8641 tggccttcca acgagcaagg gtgtggacct gatcaatggc cggcgcagcg ccatggccct
     8701 tatcgacatc gcctttcaaa ccaaaggctt gttttgggat ggccgggccc gcctattgga
     8761 agaccaggct ttgttgccgg tggaggatcc ggtggaaatg cattttacct ggcctggggt
     8821 cattgaaagg ctgaagcggc acccggaata tcctgcggat ttccggaaag ctttcggcat
     8881 cacccacaag gattccatta ccaaattcct ggctgccaaa gccatggctc agtttgaacg
     8941 aactctggtc agcagcgggg ccacccgtta cgacctggcc ctgacgcccg ggtccggagt
     9001 ctttttttcc gaaagtgaat tgcgcggcta tgaaatgtat tttgatttat ccgggggccg
     9061 cctgcccgat gcggaatgtt ttcattgtca caatgcccca ttgttttcct ccctcgatta
     9121 tttcaacaac ggcatccaac gggtggtcca tggcaatcaa tttgaagaca atggcctggg
     9181 tgccatcacc ggaaatgccg cacaaaacgg catgtttaag gcgccttccc tcagaaacat
     9241 tgcccaatcg gcgccttata tgcacgacgg aaggataaaa accctggaag cagtgatcga
     9301 acattataat tctggagggc atcccgcgga caataagaac ccttttgtcc ggaaattggg
     9361 cctgacggtg cagcaaaaag cggatttatt agccttctta catacgctga ccgataccag
     9421 ttttgcccaa aagccggatt accagtcccc tttttaattt tttttgttaa aattttaccc
     9481 cgatcggtca aagttaatgt gaggatttaa atagaaatat ctcacattaa aatataatat
     9541 tatacgtaaa tatatgtata tcataatatt aattaaatat attgtttata ttaaataaat
     9601 ttatatataa caatatgcaa aaaatgtctt tgaaacgggc gatggaggtg gcgataattg
     9661 tatcatgatt ttgattcatt tcagaatcag gattaaatac aaagttattt agtcatcctt
     9721 tttaactaaa ggatatgttc atgggattat aatttgtcaa tatggagtta tacaaaagtg
     9781 tataactcca tatttttaaa agattccatc caaaattgaa aagttctagg ttcatttaat
     9841 atatgccaat gcgtgtttgg taattttctg taccaaatcg ctggatatgt tcatgggatt
     9901 ataattgtcg gatagtcagc ccttgcaaac gtgtaggagc tgactatttt tttttggggt
     9961 aggaggttag aggtaagagg taggaggtta gaggttagtg actactattc cctgattttc
    10021 ttacgggaaa cgagcttcga gttgcgagca gtgagtttga ttgctgattg gaaacgagaa
    10081 gcgagaaacg agcttcgagc cgtgacctca ccaacttgcc aacttactaa ctagtgtact
    10141 aattataaag ttgattaact aattgttagt gcgtttcctg tatac
//
LOCUS       DAOQXT010000141        10092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_7141_length_10092_cov_7.762678, whole genome shotgun sequence.
ACCESSION   DAOQXT010000141 DAOQXT010000000
VERSION     DAOQXT010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7141_length_10092_cov_7.762678"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1071)
                     /locus_tag="PKM27_17510"
     CDS             complement(25..1071)
                     /locus_tag="PKM27_17510"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HNR09122.1"
                     /translation="MARIPQPITATVEEQQELLVMFRSHKIEKRYSQRASIILLSLQG
                     KSLEEIIQDTGLSRPVVNKWRQRFRRYGIEGLKDGARSGKPSVITAQQKALVIKKACE
                     KPTGGYTNWSQKRIAEQVGISQSKVHQILRKADLKPHKIDYWCGKSPDPEFEAKMINI
                     VGLYLTPPENAIVLCVDEKTQIQALDRTQPVLPLKEKMPKRLTTTYKRNGTVALIASL
                     AVHSGEILAKPMKRNTADNFLQFLRKLDRHYTNKMLHIIVDNYSVHKNQKVNEWLKGK
                     RKIKLHFTPTYASWLNQVEIWFNMLSKDVLKGGVWKSDEQLVSQLLEYITTYNQTRAK
                     PFAWTYTGNALTIS"
     gene            complement(1149..2945)
                     /locus_tag="PKM27_17515"
     CDS             complement(1149..2945)
                     /locus_tag="PKM27_17515"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR09123.1"
                     /translation="MSKLKANFLLLFLALTLSGLAQKPTDKRLSGLDTVVARILKDWK
                     APGVSIAVVEKNKVVYTGGFGYRDLEKKLPVTEHTLFAIGSCTKAFTSSLLGMLVKEG
                     RVDLDKPVRNYLPELKFVNDQLNNHVTLRDMMCHRTGLPRHDYSWYGSTASRMELLRR
                     IEYLEPTAELRAKYQYNNFMFMSQGVVLEKLTGKSWEENIKERIFKPLGMDQTVTDVS
                     GMEKSGDRSLAYALIRDSVALAIPYRNIDAIGPAGSINSSARDMARWLITWIGGGKYE
                     GKEILPADYVRDAMTNQMATGGLPSTDNPDIQGIGYGLAWGLASYRGHYRVEHGGGID
                     GFISTTGFFPLDSIGIYVVSCNGQVTSSVRNWIADRMLGLPYRDWHKQSRDAYVKGRF
                     TAMMTPKPNDSTGRVFNTKPSLPLGDYAGKYTHKGYGSMEVTLVGQQLRAQYNGMSLP
                     LNHKHFDIFRGRPEGPQYEDGTDTELFFALSKEGKVKSLSTPLQPGLADMVFEKELIA
                     APLTRADLEKYAGEYLLGPQQISFVIRNNTTLFALLPPQPDCELVPSGKDEFKLKIMD
                     GFSVKFKLNDQGKAIEAAFVQPNGVFTAKRKE"
     gene            complement(3195..4238)
                     /locus_tag="PKM27_17520"
     CDS             complement(3195..4238)
                     /locus_tag="PKM27_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNR09124.1"
                     /translation="MKPSMKIDLVSDTVTKPTPGMMKAMMEAELGDDVFREDPTVRRL
                     EEKMAALFGHEAALFCPSGTMTNQIALKINTQELDEVIMDVTSHVYLFELGGYSFNSR
                     VAIHLLHGEDGKFTAEDVKNAIKPKLDWMPNSRLVVIENSSNRGGGNYYTLDEILPIA
                     SFCAKNGLKLHLDGARLFNVLAETGESTRDWGRPFDTISICLSKGLGAPVGSCLIGKA
                     EAISRARKIRKVMGGGMRQAGILAAAGIYALDHHVERLKEDNDRAKRIGACLKTLPMV
                     REVKPVKTNIVIFSMKEPLTAEWMLQELAKRGVRATTIGPGAIRFVTHLDFTGEMCDH
                     LIALLEEMGNLVV"
     gene            complement(4235..5368)
                     /locus_tag="PKM27_17525"
     CDS             complement(4235..5368)
                     /locus_tag="PKM27_17525"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR09125.1"
                     /translation="MRYLFFVVLLLFLGACKNAPGLQGNAVKIFLDTEEVLPDEDTIY
                     LTGNHAALGSWNPKGLAMTRRSETRWEKTFRVPKNTHLEFKFTLGSFDRESISNYGLI
                     PNNSQLDVVEDTEVYFVLEDWKNPLLLPRVSKGSVQLHMVSPHNGLAARKVTVWLPDE
                     YASAPERRFPVIYLHDGQNQFDSTQAFTGQEWQIDETVSRLSAEGKIQAPICVAIDNT
                     FIREEEYGVVGKESPYADFVAYQLKPMIDSLYRTFPDRAHTVTMGASLGGLIAFHLAW
                     YRDEVFSAAACLSPAFKFKQINLAPELKKYKGPKKDIRLYIDNGTGGIDAQIQPGVDE
                     VMAYLKESGYPVYYQLVKGADHNESAWARRTEVPLVRFFGINQ"
     gene            5425..7215
                     /gene="lepA"
                     /locus_tag="PKM27_17530"
     CDS             5425..7215
                     /gene="lepA"
                     /locus_tag="PKM27_17530"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNR09126.1"
                     /translation="MKFIRNFCVIAHIDHGKSTLSDRLLELTHTIAAREMQDQALDNM
                     DLEREKGITIKSHAIQMNYEHSDGQKYTLNLIDTPGHVDFAYEVSRSIAACEGALLLV
                     DATQGIQAQTISNLYMAINHNLEIIPVLNKIDMEAAMIDEVSDQIIDLVGCKKEEIIL
                     ASGKTGLGVPDILTAIVERIPAPKGDPKAPLQMLIFDSVFNSFRGIVAYFRIFNGTVR
                     QNEKVRFVATGKNYQVDEIGVLQLKQVKKPELSAGNVGYIITGIKDSKEIRVGDTITH
                     VDRPGEAIKGFEVVKPMVFAGIYPIDNDDYEDLKDSIQKLQLNDAALVFEPETSAALG
                     FGFRCGFLGMLHLEIVQERLRREFNQEVITTVPNVSYYAYTTRGTKLNIQNPHELPDA
                     SMISKVEEPYIDAQIISRPDFIGSIISLCMDKRGRLVKQHYLTPQRVELQFEMPLAEI
                     VFDFYDRLKSISQGYASFDYAPSVYRESDLVKLDIKINAENVDALSALVHRDKAEALG
                     RKMCKKLKDLLPRQQFVIAIQAAIGAKIIARETISALRKDVTAKCYGGDISRKRKLLE
                     KQKEGKKKMRQIGSVEIPQKAFLDVLKLDE"
     gene            complement(7456..8124)
                     /locus_tag="PKM27_17535"
     CDS             complement(7456..8124)
                     /locus_tag="PKM27_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09127.1"
                     /translation="MGQVVKIISIEHLTHDVIRIVAEKPVTLTYNPGQAVDLSINKPD
                     WEQELRPFTFTSLPGDEHIEFTIKTYPDHNGVTQQLLSLNNKDQLIIHDVFGDISYKG
                     EGIFIAGGAGVTPFIAILKQLEKTNRVGNNKLIFANKTKADIILEDHFHKLLGNNFIN
                     ILSEEERVGYEHGYITSDLIKKHTDEHTKYFYLCGPEPMMQAVEKHLSSLGVSDHFIV
                     KEAF"
     gene            complement(8131..8778)
                     /gene="folE"
                     /locus_tag="PKM27_17540"
     CDS             complement(8131..8778)
                     /gene="folE"
                     /locus_tag="PKM27_17540"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNR09128.1"
                     /translation="MHEIPIAAVSETATDTPLHDTTFKLTDIEKINKIEYHFAKIMHT
                     LGLNLYDDSLSGTPKRVAKMFVKEMFSGLNPDNKPEISLFENRYNYKKMLIEKDITLY
                     STCEHHFVPIIGKVHVAYIPAKHVIGLSKINRLAEYYAKRPQVQERLTVQILEALKEV
                     LQHHDVAVVIEADHLCVASRGIKDTNSKTVTASYSGQFEDNAVRQEFLSQIQNNK"
     gene            complement(8786..9433)
                     /locus_tag="PKM27_17545"
     CDS             complement(8786..9433)
                     /locus_tag="PKM27_17545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR09129.1"
                     /translation="MVQLEKLTETEVIGRVLNGEKALYEIIVRRFNPHLYKVGRSYNY
                     NHEDTQDLIQDTFMDAYKNLLQFEGRSDFKTWIIRIMMNNCFRKKRKSSFKNEIMQDV
                     NDNLKPMFTSANNDTDKIVQNRELRHIIEDALGKIPFDYRMAFSLREINGLNVSETAN
                     LLSINESNVKARLNRAKTMLRREVEKTYSANELFEFNLIYCDTIVENVMKKINES"
     gene            complement(9437..9820)
                     /locus_tag="PKM27_17550"
     CDS             complement(9437..9820)
                     /locus_tag="PKM27_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09130.1"
                     /translation="MESTIKKQSLAQTFNLLKFTFGIVPIVAGADKFTNLLTNWEQYI
                     NPSIADLLPFSPSVFMMIVGVIEIIAGIIVLKKAEIGGYIVAAWLTGIALTLLIGFNY
                     LDVAVRDLVMAISAFAMVKISKLIA"
BASE COUNT         2460 a         2435 c         2435 g         2762 t
ORIGIN      
        1 ctagtgtact aattataaag ttgattaact aattgttagt gcgtttcctg tatacgtcca
       61 cgcaaagggt tttgccctgg tctgattgta ggttgtaata tattcaagga gttgtgatac
      121 caattgttca tccgacttcc aaacacctcc tttcagtaca tctttgctaa gcatattaaa
      181 ccaaatttct acctgattta gccaggaggc gtatgtgggt gtaaagtgta gttttatttt
      241 tcgttttcct ttcagccatt catttacctt ttggttttta tgaaccgaat agttgtcgac
      301 gataatatgc agcattttat tggtgtagtg cctgtctaat tttctcaaaa attggagaaa
      361 gttatctgcc gtgtttcttt tcatgggctt tgctaaaatt tctccgctgt gtacggccaa
      421 tgaagcgatc aaggcaaccg ttccatttct tttatacgtg gttgtaagcc tcttgggcat
      481 tttttctttc aacggtaaaa cgggctgtgt tcgatccaaa gcttggattt gtgttttttc
      541 atctacgcac aatacaatgg cattttccgg aggggttaaa tataatccaa cgatattaat
      601 cattttggcc tcaaattccg gatctggact tttgccgcac cagtaatcaa ttttatgcgg
      661 ctttaaatcc gcctttctta aaatctgatg aaccttgctc tggcttatac ctacttgttc
      721 agcaatccgc ttctgagacc aattggtata tcctcccgtt ggcttttcac atgctttttt
      781 aataaccagg gctttttgct gcgcagtaat caccgatggc tttccactgc gagctccatc
      841 ctttaagcct tcaataccat accgtcggaa tcgctgacgc catttattga ccaccggtct
      901 gctcaggcct gtgtcttgaa tgatttcctc caggctttta ccctgtaaac ttaataggat
      961 tatcgatgca cgttgactat atcgtttctc gattttgtgt gaacgaaaca tcaccagcaa
     1021 ttcctgttgt tcttcaacgg ttgctgtaat gggttgtggt atccttgcca tgcagcaaag
     1081 atacaaataa taagttaatt aactaagtaa ttatcacact agcaagctag caaactagca
     1141 aactaagctc actcctttcg cttcgcagta aacaccccgt tgggctgtac aaaagccgct
     1201 tcgatggctt tgccctggtc attcaactta aacttcaccg aaaaaccatc catgattttc
     1261 agcttgaatt cgtcctttcc ggaaggtacg agttcacaat ccggctgggg tggcaggagg
     1321 gcaaaaaggg tcgtgttgtt tcggatgacg aagctgatct gttgagggcc caggaggtat
     1381 tctcccgcgt atttttccaa atcggcccgg gtgagcgggg cggcaatcag ttctttttca
     1441 aaaaccatat ccgccagacc gggctgcaag ggggtggata aggatttgac ctttccttcc
     1501 ttactcaagg caaaaaacag ttccgtatcc gtcccgtctt catactgggg gccttccggc
     1561 ctgccgcgga aaatgtcaaa atgtttgtga ttgaggggaa gggacatgcc gttgtattgg
     1621 gccctgagtt gttggccgac gagagtgact tccatggatc cgtaaccctt atgcgtgtat
     1681 ttaccggcat agtcccccag ggggagggat ggtttggtat taaaaaccct gccagtggaa
     1741 tcgttgggtt tgggggtcat catcgcggtg aacctgcctt tgacataggc atcccgcgat
     1801 tgtttatgcc agtcccggta gggtaggccg agcatacggt ctgcgatcca gtttctgact
     1861 gaactggtca cctgcccgtt gcaggatacg acgtaaatcc cgatgctgtc cagcgggaaa
     1921 aaaccggtgg tggatataaa cccgtcaatg cccccgccgt gttcgacgcg gtaatggccc
     1981 cggtaactag ccagtcccca ggccaggcca tatcctatcc cctggatatc cggattatca
     2041 gtggagggca gtcctccggt ggccatctgg ttggtcatgg catcccgcac atagtcggcc
     2101 ggcaggattt ctttgccttc atatttgccc ccgccgatcc aggtgatcag ccagcgggcc
     2161 atgtcccggg cgctgctgtt gattgagcca gcgggaccga tcgcgtcaat attccggtaa
     2221 gggatcgcca gggcgacgga atcccggatg agcgcataag ccagggatcg gtcgccggat
     2281 ttttccatgc ccgacacatc ggtgacggtc tgatccatgc cgaggggttt gaagatcctc
     2341 tccttgatat tttcctccca ggatttaccg gtgagttttt ccaggaccac cccctggctc
     2401 ataaacataa aattattgta ttgatacttg gcgcggagtt cggcggtggg ttcgaggtat
     2461 tcaatcctcc ggagcaattc catgcgggag gcggtggacc cgtaccagga ataatcatgc
     2521 cttggcagcc cggtccggtg gcacatcata tccctcaggg taacgtggtt gttgagttga
     2581 tcgttgacaa atttgagttc gggcaggtaa ttcctcaccg gcttgtccag gtcgacccgc
     2641 ccttctttga ccagcatgcc gagcagggag gaggtgaatg ccttggtgca ggaaccgatg
     2701 gcaaaaagcg tatgttccgt aaccggtaat tttttctcca ggtcccggta accaaagccg
     2761 cccgtatata ccactttgtt tttttcaacg actgcgatgg agacccctgg ggctttccaa
     2821 tccttgagga tgcgggccac cacggtgtcc aggccggaga gtcttttatc tgttggtttc
     2881 tgcgccagac cggacagggt cagcgctaaa aacaataaaa ggaagttggc ttttaacttg
     2941 ctcatcggtg atttttttac caagataaaa aatcaaggcc agattccttt ccctaataga
     3001 cctgggtcgg ggaatcgccg ataagattca tttaaaccgg gttattgtca caatcagcgc
     3061 ctgttctgca cgcccggcgg tttatatcat tttattcact gcggaggtgg gcggagcctt
     3121 tgttatgtta tcccctgcgc cagctgtgag cagtcgtgct cagcggtttg aatccttgcg
     3181 aacctaagtc accactaaac cacgaggttt cccatctctt ccaataacgc gatcaaatga
     3241 tcgcacatct ccccggtaaa atcaaggtgg gtcacaaaac ggatcgcccc gggcccgatg
     3301 gtggtagccc ttactcccct tttagccagt tcctggagca tccattccgc agtgagcggt
     3361 tccttcatgc tgaaaatgac gatattggtt tttacaggtt tgacttcacg aaccattggg
     3421 agtgtcttca ggcatgctcc gatgcgcttt gcccggtcat tgtcttcttt cagccgttcc
     3481 acatgatggt ccagagcgta aataccggct gccgccagga tccctgcctg cctcatgccc
     3541 ccgcccatga ctttccggat tttgcgtgcg cgggagatgg cttccgcttt tccaatgaga
     3601 caggagccga ccggggcacc caggcctttg ctcaggcaga tggagatggt atcaaaaggc
     3661 ctgccccaat cccgggtaga ttcaccggtt tccgcaagaa cattaaacag cctggcgccg
     3721 tccagatgga gcttcagtcc gtttttagcg caaaaggaag cgatgggcag gatctcatcg
     3781 agggtataat aattaccccc tccgcggttg gaggaatttt caatgaccac caggcgggaa
     3841 ttgggcatcc agtcgagttt gggtttaatc gcatttttca cgtcttccgc cgtgaatttg
     3901 ccgtcctccc cgtgcaggag gtgaatcgcc accctggaat taaaactgta cccgcccagc
     3961 tcaaagagat aaacatggga agtgacgtcc atgatcactt cgtccagttc ctgggtatta
     4021 attttgaggg cgatctggtt ggtcatggta ccggaggggc aaaacaaagc ggcttcatgg
     4081 ccaaaaagcg cggccatttt ttcctccagc ctgcgaaccg tagggtcttc ccggaatacg
     4141 tcatccccca attcggcttc catcatggct ttcatcatgc ccggggtggg tttggtcacc
     4201 gtatcgctga ccaggtcgat cttcatggaa ggtttcattg gttgatccca aaaaaacgga
     4261 ccagggggac ttcggtgcga cgggcccagg cagactcatt gtgatcagcc cctttgacca
     4321 actggtagta caccggatag ccggattctt taaggtaggc catgacctca tcgaccccgg
     4381 gctggatctg ggcatcaatg cctcccgtac cgttgtcgat ataaagccgg atgtcctttt
     4441 tggggccctt gtattttttt aattcgggag cgagattgat ttgcttgaac ttaaaggcgg
     4501 gagacaagca ggccgccgcg gaaaaaacct cgtcgcggta ccaggccagg tgaaaggcga
     4561 tcaggccacc caggctggcg cccatggtaa ccgtatgggc gcggtcaggg aaggtgcggt
     4621 aaagcgaatc gatcatgggc ttgagctggt aggctacaaa atccgcataa ggactttctt
     4681 tgccgaccac tccgtattct tcttccctga taaaggtatt gtcaatggcc acgcagatgg
     4741 gagcctggat cttgccctcc gcactcagcc gggacaccgt ttcatcgatc tgccattcct
     4801 gcccggtaaa ggcctgggtg gaatcaaact gattctgacc atcgtggaga taaatcaccg
     4861 ggaatcgcct ttccggggcg gaggcatatt cgtccggaag ccagaccgtg actttccggg
     4921 ccgccaggcc gttgtgcgga ctgaccatgt gcagttgcac ggagccttta ctcacccggg
     4981 gaagtaacag cggatttttc cagtcttcca gtacaaaata aacctccgta tcctcgacca
     5041 cgtcgagctg ggaattgttc gggatcaatc cataattgga gatcgattcc cggtcaaaac
     5101 tacccagggt aaatttgaat tcgagatggg tgtttttcgg tacccggaag gtcttttccc
     5161 accgggtttc cgaccggcgg gtcatggcca gtcctttggg gttccagctg ccgagggccg
     5221 catggttgcc ggtcaggtag atggtatcct catccggaag gacttcttcg gtgtccagga
     5281 agattttgac tgcatttccc tgcaggccgg gggcgttctt gcaagctccc aaaaagagca
     5341 gcaggaccac gaagaacaga tacctcatga tgggcaaagt taatacttag gcaccctctt
     5401 aaaaatggta tatttgcgcc cgccatgaaa ttcatccgca atttttgcgt cattgcccat
     5461 attgaccacg gcaaaagcac cctgtcggac cgtctcctgg agctgaccca taccatcgcg
     5521 gcgcgggaga tgcaggacca ggccctcgac aacatggatc tcgagaggga aaagggcatc
     5581 acgatcaaaa gccatgcaat ccagatgaat tatgagcaca gcgacggtca gaaatatacc
     5641 ctcaacctca tcgatactcc gggccatgtg gactttgcct atgaagtttc gcggtccatc
     5701 gcagcctgcg aaggagccct gctgctggtc gacgccaccc aggggatcca ggcacagacc
     5761 atctccaacc tctacatggc catcaatcac aacctggaga tcatccccgt tctgaacaag
     5821 atcgacatgg aagccgccat gatcgatgag gtcagcgacc agatcatcga cctggtgggt
     5881 tgcaaaaaag aagaaatcat cctggccagc ggtaagaccg gcctgggagt gccggatatc
     5941 ctcaccgcca tcgtggagcg cattccggcc ccgaaagggg atcccaaagc gcctctgcag
     6001 atgctgatct ttgattccgt attcaattct ttccggggca ttgtcgccta cttcaggata
     6061 ttcaacggca cggtccggca aaatgaaaaa gtgcgctttg tggccaccgg caaaaattac
     6121 caggtggacg agatcggcgt gctccagctc aagcaggtta aaaaaccgga actgagcgcc
     6181 ggcaatgtcg gctatatcat caccgggatc aaggacagca aagagatccg ggtcggcgac
     6241 accatcaccc atgtagaccg tccgggagag gccatcaaag gttttgaggt ggtcaagccc
     6301 atggtctttg ccgggatcta cccgatcgac aacgatgatt acgaagacct gaaagacagc
     6361 attcagaaac tccagctcaa tgatgccgcc ctggtctttg aaccggagac ctccgccgcc
     6421 ctgggattcg ggttccgatg cggattcctg ggcatgctcc atctggagat cgtacaggag
     6481 cgcctgcggc gcgaattcaa ccaggaggtg atcaccacgg tgcccaacgt ttcctattat
     6541 gcctatacca cccggggcac caaactcaat atacaaaacc cgcatgaact gcccgatgcc
     6601 tccatgatct ccaaggtgga ggaaccctat atcgatgcgc agatcatcag ccgcccggat
     6661 tttatcgggt ccatcatttc cctctgcatg gataagcggg ggcgcctggt caaacagcat
     6721 tacctcacgc ctcagcgggt ggaactgcaa tttgaaatgc ccctggcgga aatcgtcttc
     6781 gacttttacg accggctcaa gtccatttcc cagggttatg cctctttcga ttatgcccct
     6841 tcggtttacc gcgagtcgga cctcgtaaaa ctggatatca agatcaacgc ggaaaatgtc
     6901 gatgccctct cggccctggt acaccgggac aaggcggagg ccctcggccg gaagatgtgc
     6961 aaaaagctga aagacctcct gccccgccag caatttgtga tcgccatcca ggccgccatc
     7021 ggcgctaaaa tcatcgcccg cgagaccata tccgccctgc gcaaagacgt taccgccaag
     7081 tgttacggcg gggacatcag ccgcaagcgg aagctgcttg aaaaacaaaa agaaggtaaa
     7141 aagaagatga ggcagatcgg cagtgtggag atcccgcaga aggcgttttt ggatgtactg
     7201 aagctggatg agtaaggggt ggcttgtgtt ggctggatcc ctgaggagag acaaaattta
     7261 aatcagcaga atcgaatcag gggcgggtca atcccgaggt tccaatcctt gactccggat
     7321 tccctgaacg gagccaggaa ccctgggtca acatgaaggg aatttacaag aaagttcatt
     7381 tatgctgagt gatccgactg gaatgtagcc tttgtcggag gcatgtggca cggtctgaat
     7441 attagaaaga attgattaaa aggcttcttt tacaataaag tggtcggaaa ctcctaatga
     7501 agataaatgt ttttctacag cctgcatcat cggttctggt ccgcagaggt aaaagtattt
     7561 ggtgtgctcg tctgtatgtt ttttaataag gtctgaggta atataaccgt gttcgtatcc
     7621 tacacgttct tcttcagaaa gtatattgat aaaattattg cctaacagtt tatggaagtg
     7681 gtcttcaagg ataatatccg ctttggtttt gttggcaaaa atcagtttgt tatttccgac
     7741 ccgattggtc ttttcaagct gttttaaaat agcaatgaaa ggggtaacac ctgctccacc
     7801 tgctataaag ataccttctc ccttatagct tatatccccg aaaacatcgt gaataatcag
     7861 ttggtctttg ttgttaagcg aaagcaattg ttgcgtaact ccgttgtggt ccggatatgt
     7921 ttttatggta aattcaatgt gttcatcacc gggtaatgag gtaaaggtaa agggtcttaa
     7981 ttcctgttcc caatctggtt tattgatgga taggtcaact gcctgtccgg gattataagt
     8041 caatgtcaca ggtttttcag caactattcg gataacatcg tgggtaagat gttctatcga
     8101 tattatttta acaacttgtc ccactgtaaa tcatttattg ttttgaattt gcgataaaaa
     8161 ttcctgcctt acagcattat cttcaaattg ccccgaatag ctggcagtaa ctgttttgct
     8221 gttagtgtct ttgatgcctc ttgaagccac acacaaatgg tctgcttcta tcacaactgc
     8281 tacgtcatgg tgttgcaata cttcttttaa tgcttccaaa atctgaactg tcaaacgttc
     8341 ctgtacttgc ggtcgtttgg cataatactc tgctaatcgg ttgattttgg ataaaccaat
     8401 aacgtgttta gctggaatat atgcaacgtg gactttgcca attatcggta caaagtgatg
     8461 ctcacaggta gaatacagtg taatgtcttt ttcaataagc atttttttgt agttatacct
     8521 gttttcaaaa agacttatct caggtttgtt gtctggattt aatccgctga acatttcttt
     8581 tacaaacatt tttgccaccc tcttcggtgt tccgctcaaa ctgtcgtcat aaagattgag
     8641 cccaagcgtg tgcatgattt ttgcaaaatg gtattcgatt ttgtttattt tttcaatatc
     8701 agtaagttta aaagtagtat cgtgaagcgg ggtatctgtg gcggtttcgc tcacagcagc
     8761 aattggtatt tcatgcatag aataatcagg attcgtttat tttcttcatt acattctcta
     8821 ctatcgtatc gcaatagatt aaattgaatt caaacagttc gttggcagag tatgtttttt
     8881 caacttcccg cctcaacatc gtctttgccc tgtttaatct tgccttaaca ttgctttcgt
     8941 tgatgctcaa taaatttgcc gtttctgata catttagtcc gtttatttca cgtaatgaaa
     9001 aagccattcg gtagtcgaaa ggaattttgc caagagcatc ctcgataata tgccttaact
     9061 cacggttttg aactattttg tcagtatcat tatttgcgct ggtaaacatt ggttttaaat
     9121 tgtcgtttac atcttgcata atttcgtttt tgaaacttga tttccttttt tttctgaaac
     9181 aattgttcat cattatacgg ataatccacg ttttaaaatc cgaacggccc tcaaactgca
     9241 acaggttttt atatgcatcc ataaaggtat cctgtattaa atcctgcgtg tcttcgtgat
     9301 tataattgta tgacctgccc actttgtaca gatgtggatt gaaccgcctc acgataattt
     9361 catatagagc tttttccccg tttaaaaccc gtccgataac ttctgtttcg gttaattttt
     9421 caagctgaac cataggttat gcaatgagtt tagatatttt caccatagca aaagccgaaa
     9481 tagccattac aaggtcgcgc acagctacat ccaaatagtt gaaaccaatc aacagtgtta
     9541 aagcaatacc agtgagccac gcggctacga tatatccgcc aatttcggct tttttcagca
     9601 caatgatacc ggctataatt tcaataacac ccacaatcat cataaagacc gacggagaaa
     9661 acggcaatag gtcagctatt gaagggttaa tgtattgttc ccaatttgtc agcagattgg
     9721 tgaatttgtc ggcccccgca acgattggga caattccgaa tgtgaacttt aataggttaa
     9781 acgtttgtgc cagcgactgt tttttgatcg tactttccat tttttgtctt tttaagaagt
     9841 tactatatga ttagagtaaa cgaactttgg aaaggttaca attaatttag actggatgct
     9901 gaaattttaa cactggtgtc tttggcaatt actgccaaag gttacagcgg ggacatcagc
     9961 cggaaatgaa aactgcttga aaaacagaag gaaggcttaa agaagattcg gcagatcgga
    10021 agtgtggaga tcccgcagaa ggcgtttttg gatgtgctga agctggatga ttaggggagg
    10081 gctgatgtaa aa
//
LOCUS       DAOQXT010000142         9871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_7349_length_9871_cov_6.395884, whole genome shotgun sequence.
ACCESSION   DAOQXT010000142 DAOQXT010000000
VERSION     DAOQXT010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7349_length_9871_cov_6.395884"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKM27_17555"
     CDS             complement(<1..860)
                     /locus_tag="PKM27_17555"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HNR09131.1"
                     /translation="MNFPYHTKILRKAVFAYSFFFFCPGLFSQSYWSAAPPGVQDLPG
                     EKKTRFILDTFVFFENLRKAEAVSLPVGTNQFIESTLSPCSNFAPGLAAKYPSIQTYC
                     NSKAGATVRLDLYKQKLTAFILHEGRTYFIEPDSLPGFYVFSEEKAQASGLHQTFECS
                     TLEPVSDPTLLNPAKFRGVPGQNSSLKTYRLALALTGEFSRTHGNTVEAVLAALNSHL
                     SRINAVYVNENAIQFQLVANNDTLLFMDPDHDPYVNGNVVQMLEQNPKVLNDRIGLNN
                     YDVGHVFGTNA"
     gene            complement(951..>2156)
                     /locus_tag="PKM27_17560"
     CDS             complement(951..>2156)
                     /locus_tag="PKM27_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09132.1"
                     /translation="ANEWQFEQNWQLARNTLWAFTLSSRSLRFDKIVNPLSDKDTRSL
                     VLRHSLNTRTRKDFIRFSQTLESGSGQEPALEYTYLRVNKGQGYYAWIDINGDSAIQV
                     SEFDLAPFQDQGEYIRYAVTGNQFIRTRNHLFLQNLEIDPSRLSSNKGSLKWWQRFAL
                     VSNFNVGWKQTGDHGFRSPFHADSGLAGLQAQIRNQVFFNRNRPGFELQAGQLINQNK
                     QLLTTGFENRDLIEYFFRSRLLIHSRVSLESYLAKQQQKSELEFFDQRNYSITSHTME
                     PRIQIQASTAMRLTLAFRQKWSHNRMGSEKSNAREVRLEGLSQPLNKWSVRSSCSLVG
                     INYDGENNAWIEYSMLQGLRPGNNLLLSVNVDREIQKNTMLRLGYDGRKSEKSRFIQT
                     GRVQVIASF"
     assembly_gap    2157..2256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2257..4259)
                     /locus_tag="PKM27_17565"
     CDS             complement(<2257..4259)
                     /locus_tag="PKM27_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09133.1"
                     /translation="MKDRQPLRRSRPSGWLFLGISLSWYACFFKAEAQSSSTLRNGDY
                     LIRDSLRLRDSLYIIPGSVTIRQKNGNSFEGQYQVSGKLLFITRIHSDSLPVNVQYRV
                     LAYDPDLRVYAGDTAWLPAATAELPMSYKFSKSRPVMRQNPLDEVDYAGAFGRGLSFG
                     NNQSLVLNSNINLQVNGKLGNDIEIHAAISDENIPLQADGNTQQLNEIDQVFIEIKRK
                     NQSLLAGDQQLSPDGTYFMKYQKKYKGLRYSNEHQFGDGGRLSTQVHGSIARGNFKRQ
                     ILEIQEGNQGPYRLTGTSNELFVIVLSGSEKVYLDGLLLARGLEQDYVMDYNRSEISF
                     TSRRMLNRNSRVVVEFEYSNQSYLKSAWSLQSKYQQNDLTVQWNSYLEKDNKSSNILQ
                     DLSQEDIQILKDAGDQISQLKRPTLREIGQGSPADQVSYNLKDSLVSQVLYSGILVAA
                     SQANRGSHTAQFSYVGPGQGNYVLETGLFTNGRAYRWVSPDPVTKEKRGEYEPISSLS
                     APKQQSQHALRLEYTPQSGLFSRTELSLSDLDLNLYSTKDKKDDLGLAWKQELGLRKY
                     LGAGDLSWTSTFGLERINRRFSYFEPFRNPEFNRDWSLNTGLPSTANELLWSARSQLK
                     NKAVDVQYQYQHYGREQDYSGSRHSWNGQFHLKSTEISSYGSLA"
     gene            complement(4376..5272)
                     /locus_tag="PKM27_17570"
     CDS             complement(4376..5272)
                     /locus_tag="PKM27_17570"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNR09134.1"
                     /translation="MKRLVYFFFRGMIALLSVLPDRGLYMVSAVLRILLLNILQYRKK
                     VVDRNLMNSFPALSPSSLAAIRRKYYQNLADLLIESLASYGWTVDRIHRHFRYLNPEL
                     LQEYIAQGRMVIGVTAHTGNFELGAVLTQPVLGLPCYAVYRPLANPHIDRFIYKKRSG
                     QGITLRSDKKTGELIDMMAPPAILFLIADQNPSIISKAKWVPFLNQDTAFVHGPATIS
                     KMRNLPVIYFDVVRIRQGYYECTLSKLIENPAELTEQEILERYASKLESCLNTHKDDW
                     LWSHKRWKWKREGGTVVRNPVY"
     gene            5339..6292
                     /locus_tag="PKM27_17575"
     CDS             5339..6292
                     /locus_tag="PKM27_17575"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HNR09135.1"
                     /translation="MKNRRQFLKMAPTLAGLPLAGLDDKPTVQPPVIVSTWDFGKDAN
                     EAALRVLETQGSALDAVESGVHVPENDPANQSVGYGGLPDRDGIVTLDACIMDHRGRA
                     GSVSGLEHIRHPISVARRVMEKTPHVMLVGDGALQFALSEGFQKENLLTDKSKEAWEK
                     WRVTSKYQPQINIENHDTIGMIVWDHKRQFAGACTTSGLAYKMHGRVGDSPIIGAGLY
                     VDQAVGAATGTGLGEVVLRQCSAFLIVELMHNGKSPQKACEEAIRRIVLANANYRDFQ
                     VGFIAVNSKGQVGAYSIQPGFTYALYQNGKHQILPSKSYLV"
     gene            6289..6939
                     /locus_tag="PKM27_17580"
     CDS             6289..6939
                     /locus_tag="PKM27_17580"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR09136.1"
                     /translation="MTHTLSIIYPQPIFAAGLVKTIENHPVMSFQCVGQTSSAREALI
                     RASDWITDLVIIGIHSKGLEELKLIEAIRKKSKKVHILIIAGQEHLDVVRRGLVKGAN
                     GFIYAHSDPPVLLEAIEAIFQKQVFLPGGFRIHPVPVPAPNTLRISGRADFHITDREI
                     QVLKLICRGFNNKQISNDLFISVQTVAVHRKNLFRKLGVKNSISLIQLAMEKKMIG"
     gene            7122..>9871
                     /locus_tag="PKM27_17585"
     CDS             7122..>9871
                     /locus_tag="PKM27_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09137.1"
                     /translation="MSVLLIFSFTPIQGQPKNLIHGTNKARDSAQVFTGDKYFDSGGP
                     GGSKLTDQPGNYSNCSDPFNENANCTSYFTLCSNEDTVSVNFIAYQIVTGDRLRIFSG
                     SKPSGPLLFNSLTQGVSLNGMKLTTGTYIKSKAPDGCLTFEWFCTTIANSIGWEADII
                     VIGKNQPEDSLCNPSCKPLALVRIPLDTCYKLLGANELILDYHPTCAYSLQLYYPFGS
                     DQLSPPAVNATHLNNTFLYEIKSGDGPTCFGYIQVEQESLPVEICGRDTVDCTWWQKN
                     QDLKPEVSTCSGIRYSIRKTTFISLDCDDQFSGMVVREISFGGIPDSICIDTLFLIKP
                     FLDSLVCPSDLKVPCNLEGLDKDPAKLSPSHLMTILDMDGDRRMDADPARLLIPEWKG
                     NGALDTSGACGMVVSWNDFLIPGCGNSFKIRRQWTIGYKCGGRDTVCIQNISIVDESP
                     PELAAVTSMEFVADPSACKASVMLDSVPGIEDCNKVQQRLEITYPDPVNPGKQILISG
                     ALPIKLSLAPGGYKAKFIFSDPCFNAVRQEVCIQVADYQGPVLKLKQQDFFLEPQMCW
                     RRVYAGDLDSSSADLCCAKLHFAIAYEDTLSHYREQWLDWIAGNCQGTDGYPAHKDFY
                     DRLIDQWLTAFVFDDYLDLAACESRQVVVRAYEACRIPVPDPSFKGSGHQWFCYMTIP
                     EFRGWMNGGGRIGQYDLMCHSAWTAGEDLSFKYPSLEALNDSISCSALYDRENLNRIL
                     LQYDEKTVPLRIRDTSSPEIGSLPDLVVYVDGMPDANITCTGETYQAGSWPGAITCNC
                     DTGHFETFYGGPLRNDAVYDEQGQYHYPACQPYDPSIKPRPIYCSSLLKAEYTVPGSL
                     VPDSLFYQPVFGTVAQGRQFHITGTCTEGYTVSYQDVPTLDPCGAGTIIREWTITNAC
                     GQT"
BASE COUNT         2355 a         2410 c         2514 g         2492 t
ORIGIN      
        1 gcattggtcc cgaatacatg gccgacatcg taattgttca gcccgatccg gtcattcaaa
       61 acctttggat tctgctccag catttgtacg acatttccgt tgacatatgg atcatggtca
      121 gggtccataa agagaagggt gtcgttgttg gccaccagtt ggaactggat ggcattttca
      181 ttgacatata ccgcattgat cctggaaagg tggctgttga gggccgcaag cactgcttca
      241 acggtattcc catgcgtgcg cgaaaactca ccagtgagag ccagggccag gcgatatgtt
      301 ttcaggcttg aattttgacc ggggacgcct ctgaatttgg ccgggttcag cagggtgggg
      361 tccgaaacgg gctcaagggt actgcactca aacgtttgat gcagaccgga tgcctgagcc
      421 ttttcttctg aaaaaacata gaagcccgga aggctgtcgg gttcgataaa ataggtccgg
      481 ccttcatgga ggatgaaagc ggtcagtttt tgtttgtaaa ggtcaagccg gacggtggcc
      541 cccgcttttg aattgcaata agtctgaatg gacggatact tggcggccag cccgggagcg
      601 aaattggaac agggggaaag cgtggattcg ataaattggt ttgttcctac cggcagcgat
      661 accgcctcag ccttccgaag gttttcaaaa aatacgaaag tgtccagtat gaaccgggtc
      721 ttcttttcac ccggcaaatc ctggaccccg ggtggcgcgg cactccaata agattgtgaa
      781 aaaagccctg gacagaagaa aaaaaacgaa taggcaaaaa ctgcctttct caatattttg
      841 gtatggtatg gaaaattcat cggccgtatt caattccggg caaaggtaaa gtctccggaa
      901 atggcaaggc cgggacaaat actgcgagga ggataggggt ttggacttac ttaaaaactg
      961 gcgatgactt gtacccggcc ggtctggata aaccggcttt tttcactttt ccggccatcg
     1021 taacccagtc tcagcatggt gtttttctgg atttcccggt caacattcac actcagcaag
     1081 agattgttac ccgggcggag gccctgcaac atggaatatt cgatccaggc attgttttca
     1141 ccgtcgtagt ttatccctac caggctgcag gaggaccgga cgctccattt attcaatggt
     1201 tggctcaggc cttccaggcg gacctcccgg gcattggatt tttcactgcc catccggttg
     1261 tggctccact tttgtctgaa ggccagggtc aggcgcatgg cggtgctggc ctggatctgg
     1321 atccgaggct ccatggtatg gctggtgatc gaataatttc tttgatcaaa aaattccaat
     1381 tcgctttttt gttgttgttt cgccaggtag gattcgaggc tgaccctcga atggatcaga
     1441 agcctcgaac ggaaaaaata ttcaatgagg tcccggtttt caaaacccgt ggtcagcagt
     1501 tgtttgtttt ggttgatcaa ttggcctgct tgcaattcaa aaccgggccg gttccggtta
     1561 aaaaaaacct ggttccggat ctgggcctgc aaacctgcaa ggccgctgtc cgcatggaat
     1621 ggactcctga acccatggtc acccgtttgt ttccatccga cgttaaaatt tgaaacaagg
     1681 gcaaaacgct gccaccattt cagggaaccc ttatttgagg aaagccttga ggggtcaatc
     1741 tccaggtttt gtaaaaacaa atgattccgg gtgcgtataa attggttgcc cgtgacggca
     1801 tagcggatat attccccctg gtcctggaaa ggggcgaggt cgaattcgga aacctggatc
     1861 gccgaatcgc cattgatatc gatccaggca taatagcctt gtcctttgtt gactctcaga
     1921 taggtgtatt ccagggccgg ttcctggccc gaccccgatt ccagggtctg ggaaaagcgg
     1981 ataaagtcct tccgggtacg ggtattcagg gaatggcgaa ggaccaggga ccgggtatct
     2041 ttgtcggata ggggattgac gattttatca aaccgcaggg atctactgct cagggtaaaa
     2101 gcccaaagcg tattgcgggc aagttgccag ttttgttcaa actgccattc attggcnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcca gggacccata actgctgatt
     2281 tcagtggatt tgagatggaa ctgcccgttc caactgtgac gggagcccga atagtcctgt
     2341 tcccttccat aatgctggta ttgatattgg acatccacgg ctttattctt caactggctg
     2401 cgggctgacc aaagcaattc attggctgtt gagggaagcc ccgtgttcag gctccagtcc
     2461 ctgttgaatt caggattgcg gaacggttcg aaataactga aacgacggtt gattctttca
     2521 agcccaaagg tactggtcca gctcaggtca cctgcgccca aatacttccg gaggcccagt
     2581 tcctgtttcc aggcgagtcc gagatcatcc tttttatctt ttgtggaata gaggttgagg
     2641 tcgagatcgc tgaggctgag ttctgtcctg ctgaacaaac cggactgggg cgtgtattcc
     2701 aggcgcaagg cgtgctggct ttgctgtttg ggggcggaga ggctgctgat gggctcatat
     2761 tccccccttt tttccttcgt gacaggatcc ggggagaccc atcgataagc ccttccattc
     2821 gtgaacaatc cggtttcaag tacatagtta ccctgtcccg gcccgacata actgaactga
     2881 gcggtatggg aaccgcggtt ggcttggctc gccgcaacca gtatgcctga ataaagaacc
     2941 tgggaaacca ggctgtcctt tagattataa ctgacctggt cggccggtga gccttgcccg
     3001 atttcgcgga gggttggcct tttcaactgg cttatttggt cacctgcatc cttgagtatt
     3061 tggatatctt cctggctcag gtcctgcagg atattggagg atttattgtc cttttcgaga
     3121 taactgttcc attggacggt gagatcgttt tgctggtatt tggattgcag gctccaggct
     3181 gatttcagat acgattggtt ggaatattca aattcgacca cgacgcggct gttgcggttg
     3241 agcatccttc tcgaagtaaa actgatttca gaccggttat agtccatgac ataatcctgt
     3301 tccagtcccc gggccaggag caggccatcc aggtagacct tttcggaacc ggacagaaca
     3361 atgacaaaca gttcattgga ggttcctgtg agccggtatg gaccctggtt gccttcctgg
     3421 atttccagga tttgtctttt aaagttgccg cgggctatgg agccatgcac ctgggttgaa
     3481 agacgccccc catcgccaaa ctgatgttcg ttggaataac gcagcccctt gtatttcttc
     3541 tggtacttca taaaataggt gccgtccggt gaaagttgct ggtccccggc caacagggac
     3601 tggttttttc tttttatctc aatgaacacc tggtcgattt cattgagttg ctgagtattc
     3661 ccgtccgcct ggagggggat gttttcatcg ctgatagcag catggatctc aatatcattg
     3721 ccaagttttc cattgacctg caggttgatg tttgaattca gcacgagact ttgattgttg
     3781 ccaaaactca atccccggcc aaaggctccg gcgtaatcca cttcatccaa tgggttttgc
     3841 cgcatcacgg gccttgactt tgaaaactta taactcatcg ggagctccgc cgttgctgcg
     3901 ggaagccagg cggtgtctcc cgcatagacc cggagatcgg ggtcataagc gaggacccgg
     3961 tactggacgt tcacgggcag gctgtcggaa tggatccggg tgatgaacag taatttaccc
     4021 gatacctggt attggccttc aaagctgttc ccattttttt gcctgatggt caccgaacct
     4081 ggaatgatgt aaaggctgtc acgaaggcgg aggctatccc ttatcagata gtctccgttt
     4141 cgaagtgtac tggaactctg ggcctctgct ttgaagaaac aggcatacca ggacaggctg
     4201 atccccagga acagccaccc ggatgggcgg gagcgtctca acggttgcct gtccttcata
     4261 ccctctggtc gacgaagtta ggttcatttc agggaagccg gaacccggaa atgccccggg
     4321 tcgggaagcc tggaatgata aatctcaatg cgggcagaag ctgaactccg gatgttcaat
     4381 agactggatt tctgaccact gttcctcctt ccctcttcca tttccagcgt ttatgggacc
     4441 aaagccagtc atccttgtgc gtattcaggc aactttcaag tttcgaggcg tatcgttcga
     4501 ggatttcctg ttccgtaagc tcggccgggt tttcgatgag tttggacagg gtacattcgt
     4561 agtatccctg cctgatccgg acgacatcaa aataaatgac aggcaggttc cgcattttcg
     4621 atatcgttgc cgggccatga acgaaggccg tatcctggtt caaaaagggt acccattttg
     4681 ccttgcttat gattgaggga ttctggtctg caatcaggaa aagaatggca gggggagcca
     4741 tcatatcgat cagttcgccc gtttttttat ccgatctcag ggtgatgccc tggccggatc
     4801 tctttttgta aataaaccgg tcgatatgag gatttgccaa tggacggtat acagcatagc
     4861 agggcagtcc gaggacaggc tgggtcagga ccgctccgag ttcaaaattc ccggtatggg
     4921 ccgtgacccc tatcaccatc cgtccctggg caatgtattc ctgcaacaat tcgggattca
     4981 aatacctgaa atggcggtgg atccgatcga ccgtccagcc gtaagaggcc aggctttcga
     5041 tcagcaggtc ggccagattt tgataatatt ttcttcttat ggcagcaaga ctggagggag
     5101 ataaggccgg aaaggagttc attaaattcc ggtcgaccac ctttttgcgg tattgcagaa
     5161 tatttaataa aaggatcctt aatacggctg aaaccatata gagaccccgg tcaggaagga
     5221 cggaaagaag ggcgatcatc ccccggaaaa agaaatatac cagccgtttc atcagccgcc
     5281 aaaattaatt accttgcttg gtagaagtgt cattttagcc cggcagaacc cagaactaat
     5341 gaaaaacaga agacaattct taaaaatggc tccaaccctg gccggtcttc ccctggccgg
     5401 tctggacgac aaaccgacag tgcaaccgcc ggtcattgtt tccacctggg attttggaaa
     5461 ggatgcaaat gaagcagccc ttcgggtact cgaaactcag ggttcggccc tggatgccgt
     5521 agaatcagga gtgcatgtac ccgaaaacga tccggccaat caaagtgtgg gatatggcgg
     5581 attgccggat cgggacggca ttgtgaccct ggatgcctgc atcatggacc accggggaag
     5641 ggccggttcg gtcagcggtt tggagcatat caggcatccc atttccgttg cccgccgggt
     5701 gatggagaaa acacctcatg tcatgctggt gggtgacggg gctcttcaat ttgccttatc
     5761 cgaaggtttt caaaaagaaa atctgcttac cgataaatca aaggaagctt gggaaaaatg
     5821 gagggtcact tccaaatacc agcctcagat caatatcgaa aatcacgata ccattggtat
     5881 gatcgtttgg gatcataaaa ggcaattcgc aggggcatgc accaccagcg gccttgctta
     5941 taaaatgcac ggcagggtgg gagacagccc gattatcggc gcgggccttt atgtcgacca
     6001 ggccgtgggg gcggccaccg ggactggact gggtgaagtg gttttacgcc agtgttccgc
     6061 tttcctcatc gtggaactga tgcataacgg aaaatcccca caaaaagcct gtgaagaagc
     6121 catccgccgg attgtactcg ccaatgccaa ttaccgtgat ttccaggttg gatttattgc
     6181 tgtcaacagc aagggccagg ttggtgctta ttccattcag cccggcttca cctatgctct
     6241 gtaccagaat gggaagcacc agatcctgcc ttcaaaatcc tacctggtat gacccatacc
     6301 ctttccatca tctatccaca gcctatattt gccgccggtc ttgtgaaaac tattgaaaac
     6361 catcctgtta tgagttttca atgtgtcgga caaacttcat cggcacgtga agccttgatc
     6421 agggcttctg actggatcac cgacctggtc attataggaa tccattccaa aggacttgaa
     6481 gaactgaaac tcattgaagc catccgtaaa aaatccaaaa aggttcatat tctgatcatt
     6541 gccgggcagg aacacctgga tgtggtccgt cgcggcctgg ttaaaggggc aaacggattc
     6601 atctatgctc attccgaccc gcctgttctc ctggaagcca ttgaggctat ctttcaaaaa
     6661 caagtcttcc tccccggagg attccggatc catcctgttc ccgtcccggc tcccaataca
     6721 ctgaggatca gcggaagggc cgatttccat attacggacc gggagatcca ggtccttaaa
     6781 ctgatctgcc gcgggttcaa caataaacaa atttcgaacg atctgtttat ttccgtgcag
     6841 accgtagccg tgcaccgcaa aaaccttttc aggaaattgg gggtcaaaaa cagcatttcc
     6901 ctgatccagc tcgcgatgga aaagaaaatg atcggctgag gcaggaaaat ttaaccaaaa
     6961 aggggataaa tcccattgat aatcaagcta cttttgtccc gattgctcaa ttttgggtga
     7021 tccgaattta gaaccagaac ttagacaatt ggagtaaagt gcaatgcttt cactcatatc
     7081 ccttttttaa taaaagctta cataagcttt tagcgcctgg tttgtctgtt ttattgattt
     7141 tcagttttac cccaatacag ggccaaccta aaaacctcat tcacggaaca aataaagcga
     7201 gggacagcgc ccaggttttc accggcgata aatactttga ttccggaggg cccggtggaa
     7261 gtaaacttac ggatcaaccc ggcaattatt ccaactgttc cgatcctttc aatgaaaatg
     7321 ccaactgtac ttcttacttt accctttgtt ccaatgagga tacggtttca gtaaatttta
     7381 ttgcctacca gattgtaacc ggcgaccggc tcagaatttt ttcaggttca aaaccttccg
     7441 gccccctgct tttcaacagc ttgacccagg gggtttccct gaatggaatg aaactgacca
     7501 ccgggactta tatcaaaagc aaagcccccg acggctgcct caccttcgaa tggttttgta
     7561 ccaccattgc caattccatc ggatgggagg cggacatcat cgtgattggg aaaaaccagc
     7621 cggaagacag cctttgtaac ccttcctgta aaccgcttgc tttggtccgg attcccctcg
     7681 atacctgtta taaattatta ggagcgaatg aactgatcct ggattaccat cccacctgcg
     7741 cctattcgct tcaactgtat tatcctttcg gatcagacca gttgtcgcca cctgccgtaa
     7801 atgccaccca tctcaacaat accttcttat atgaaataaa atcgggggat gggccgactt
     7861 gttttggtta tattcaggta gaacaggaaa gtttgccggt tgaaatctgc gggagggaca
     7921 cggtggattg cacctggtgg cagaaaaacc aggatttgaa accggaggtc agcacttgtt
     7981 ccgggatccg gtattccatc aggaaaacta cgtttatctc tctggattgt gatgatcagt
     8041 tttccggaat ggtggtccgg gaaatcagtt ttggaggaat ccccgactcg atttgtatcg
     8101 ataccctctt cttgatcaag ccgttcctgg actcactcgt gtgcccatcc gatcttaaag
     8161 taccctgtaa cctggagggc ctggacaagg atccggcgaa actgtcgcca agccacctca
     8221 tgaccattct tgatatggat ggggaccgtc gaatggatgc ggatcctgcc cgcctgctca
     8281 tccctgaatg gaaaggcaac ggagccctcg atacttccgg tgcttgcggc atggtcgtct
     8341 cctggaacga ttttctgatt ccgggatgcg gcaatagttt taagatacga aggcagtgga
     8401 cgattggtta taaatgcggc ggcagggata cggtctgcat tcaaaacata agtatagtgg
     8461 acgaaagccc tcccgaactg gctgcggtca cttccatgga gtttgtggca gatccttcag
     8521 cctgcaaagc ctctgtgatg ctggattcgg tgccgggaat agaagactgt aacaaagtgc
     8581 agcaacgttt ggaaatcacg tatccagacc cggtaaatcc gggcaagcaa attcttatct
     8641 cgggcgccct gcccatcaaa ttatcgttgg ctccgggagg atataaagct aagttcattt
     8701 tttctgatcc ttgtttcaac gcagtacggc aggaagtttg tatccaggtg gccgattacc
     8761 aggggccggt attaaaatta aaacaacagg acttttttct cgaaccgcag atgtgctgga
     8821 gaagggttta tgccggggat ctggacagtt ccagcgcaga cctttgctgc gccaagctgc
     8881 attttgccat tgcctatgag gatacacttt cccattaccg ggagcaatgg ctggattgga
     8941 tcgcgggaaa ttgccaggga acggatgggt atccggcgca taaggatttt tacgaccggt
     9001 tgatcgacca atggctcacc gcatttgtat ttgatgacta cctggacctg gccgcctgtg
     9061 aatcccggca ggtggtggtc cgggcctatg aagcctgcag gattcctgtt ccggatccct
     9121 cttttaaagg cagcggccat caatggtttt gttatatgac cattcccgaa ttccgcggat
     9181 ggatgaatgg gggcggccgg attggtcaat atgatttgat gtgccattcg gcctggacgg
     9241 ctggtgaaga cttgtctttc aaatatcctt ccctggaagc cctaaacgat tccatttcct
     9301 gcagcgcctt gtacgaccgg gaaaacctca accgcatcct tttgcaatat gatgaaaaaa
     9361 ccgtacctct ccggatcagg gatacctcaa gccctgaaat aggcagcctt ccggacctgg
     9421 tggtctatgt tgacggtatg ccggatgcga atatcacctg caccggcgag acataccaag
     9481 caggaagctg gcccggagcc attacctgta attgtgacac gggccatttc gaaacctttt
     9541 atggaggccc gctccgaaac gacgcggtct atgatgaaca gggtcaatac cactatcccg
     9601 catgtcagcc ctacgatcca tcgatcaaac ccagacctat ttattgcagt tccttgttga
     9661 aggcagaata tacggttccc ggatccctgg ttccggattc cctgttttac caacccgttt
     9721 tcggcaccgt ggcccaggga agacaatttc acatcacagg gacttgtacg gagggttata
     9781 cggtttcata ccaggatgtc cccactctcg acccctgcgg agccggaacc attatcaggg
     9841 aatggaccat caccaatgcc tgcggccaga c
//
LOCUS       DAOQXT010000143         9861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_7357_length_9861_cov_6.046400, whole genome shotgun sequence.
ACCESSION   DAOQXT010000143 DAOQXT010000000
VERSION     DAOQXT010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7357_length_9861_cov_6.046400"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /gene="folK"
                     /locus_tag="PKM27_17590"
     CDS             complement(<1..369)
                     /gene="folK"
                     /locus_tag="PKM27_17590"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNR09138.1"
                     /translation="MATPKQVFLSLGSNLNQPVRQLKKACHLIESRIAGIQVSSSIYK
                     TQAWGHLEQPDYFNQVVCIASTRPASRILELLLQIESFMGRIRQGIWEPRIIDLDILF
                     YGREIIKTPKLIIPHPRLHER"
     gene            complement(443..2089)
                     /locus_tag="PKM27_17595"
     CDS             complement(443..2089)
                     /locus_tag="PKM27_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNR09139.1"
                     /translation="MNLTATILYSMVSLVVLIATVYFFFRVVSIPGKTIKSDTGWGSR
                     IGLVLAMAGNAVGLGNFLRFPVQAINNGGGAFIIPYLVCFLLIGIPLLFVEWSMGRFG
                     GTPYRNRPMGDHSVPFILQRVHAARWVKYIGVFGIFTNIAVAAYYCYIESWTMSYVFH
                     SITGTFRGMSQEGVSSFFDVYVDLGQSTLGLPYEPVVFYILCLALNVYILSRGLERGV
                     EKVAKIGMPLLILFAMLLAIQTLSLQPGEKGAVYAGTEGLRYLWTPDFSSIWKPSVWI
                     AAAGQIFFTLAVGMGTIQCFSSYLKKDDDTALNAMSAGWMNEFVEIVLGSAIIIPMAV
                     GYLGIDGMTDIVRNLGGYGLGFKTLPYLFQQWGPLLATFAGMMWFGLLFFAGVTSSLA
                     MGTPVIGFLKDEFNIPFSRGAWIFGLITLIMGMPTVIFYKEGVFTEYDDWAGTYSLVI
                     FAFAEIVLFAWIFGIDRAWEEITRGADIRVPVVYKYIIKFVTPVLLGIILFSNIPGMF
                     QRVLHPLNGYVSFAQIYLLALFLFIVYWVHKAHLKRLGLN"
     gene            complement(2660..5689)
                     /locus_tag="PKM27_17600"
     CDS             complement(2660..5689)
                     /locus_tag="PKM27_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09140.1"
                     /translation="MFWKTCLTHTGEILLALGIAWLLGYLWNRWFGQKKDPVDKKEYE
                     DQIRSLRERIKMQDEEIRMSKASQDHWASERAGFQHNLSSLQNEIDTLTNQFKEYVSP
                     SELSSLQNRFEESLKAAEEKSNALTAERKGLLSSLASVENQLQLASKKADESQARWKQ
                     AEADLSQSVSATDRLEQEISQLANRLNQQASDHKLQEDTISNLRDELENRDKQIRELS
                     EALTDAQQKNKETTAHFQDLLASKEQELGLLAQQQATKEETTPKPGETDQLLQEISEL
                     KSELARVRNEEQNTERRLQEYQVQHHQLELELSALKSEPNQLAQLSQKSSDLEKALVE
                     SNRKVQEWEARWKEAAMDAEHISAAHASLLKEKDNQSGRITELENELDQLRTASQERL
                     KEDAEWTTRQQDFIEKLQRGQDFEKAYASANEELNSIRQELAQKEQVLAELKISREEF
                     EAQQEEYEQKLKASQLQLENYEQSVQQQQQVLRELRQKLEQSQQELVHSKRQYQDQAS
                     QTGDWEQKLNSTQQALDAARAQLAENKTNQSVLETLISEWEKKYHSVQKELHQTEQQL
                     SSSLQTSNSQANSLITWETRFKELQDALEATQLQHSQSLKHQNTLEAIIAEWERKYYD
                     ARVDLQHAQEQLNEHLRAKETEAASIGEWEDRFLGLQSELATVQNNLNETLVERDRNG
                     ESLREAEKRFKELSDKFNIVQLQLADSQRQNSHLEEDIEKAKEYQLKYNQESRAWQKR
                     LQDLEKEHAERIAGIEARFNQEIKDISNEREILKVKLASPVPVPVRKPEPETKEPGPE
                     PTGVDWRAVSSLMGKKIKPDDLKIIEGIGPKISNLLRRNQVRSWSALSEQKVEDIQAI
                     LGKGGTSFTLADPTTWPEQARLAASGDWQKLKKLQEKLYAGRLKKPKKKKLPVLTPAD
                     FEAASEVFNRKIKQDDLQIIEGIGPKIAALLRKEDIRTWQALSATRVKTLQLILEQAG
                     NKFNLADPTHWPAQAKLAARGKWKKLKAFQDQLIAGK"
     gene            complement(5702..6508)
                     /locus_tag="PKM27_17605"
     CDS             complement(5702..6508)
                     /locus_tag="PKM27_17605"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR09141.1"
                     /translation="MRKTLILLATFLWLAFWWWWYTCKICTTCLCARKEVPVIAAPAQ
                     EQGVVLFNHGDSIALIQPGWPAFRDSLILAMAPAKVLEIEGHFLDEEPNPTGFANLGL
                     ARAAALSFLFPDSIRPRISRTSLRIDKREGMDLYPFRASSWNIKPAPGKIVETEGKIL
                     IYFQSNSNQRIKDPEIETYLSNLANEHRNARSVFEITGHTDDQGPEKLNTRLGLARAG
                     SVKNFLVGKGIEASRIRIFSKGESDPIAGNDTEEGRRLNRRTEIEIIQKL"
     gene            6587..7918
                     /locus_tag="PKM27_17610"
     CDS             6587..7918
                     /locus_tag="PKM27_17610"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNR09142.1"
                     /translation="MSAYPHSIFWFRRDLRLNDNHALYQALSQSDQVLPVFIFDTHIL
                     KDLRDPQDKRVQFIYQELCGINKKLEKRACAVRLYFGEPVKILEKLCTQFHIHAVFAN
                     EDYEPYGRTRDRDVAGMLNKKKIPFHLFQDHVICRPDEILKHDGKPYTIYTPYMNKWR
                     EGVGSKTIASYPSEKLLNKVTPGIQTPTLSLEAIGFQSIDCRYPDRRISGERLRSYAR
                     TRDRIPDESGTSHLGIHLRFGTISIRELFQQALSFRAEVFINELIWREFFIQILFHFP
                     RVVHSSFKPQYEGIPWRNDPGDFKLWCQGQTGYALVDAGMKHLNKTGQMHNRVRMLVA
                     SFLCKHLLIDWRWGEAYFAEKLLDYELASNNGNWQWAAGSGCDAAPYFRIFNPEEQRK
                     KFDPQSAYVLKWAPEYGEFNQPVPMVDHRFARERALMVYKRTLEESRQGLK"
     gene            complement(8320..>9861)
                     /locus_tag="PKM27_17615"
     CDS             complement(8320..>9861)
                     /locus_tag="PKM27_17615"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNR09143.1"
                     /translation="EQELELAKKLLPTIDLLEINLLPKKWIRDEGRVIIITGPDKPSL
                     SYPTEETILKMATEIKGMEIGPYEDQVSDEPLISRDFTAVEIISEKKDEKLGTTEFIL
                     KNGVTVILKKTDFKNDEILMAAYSPGGTSLYREEEDVDASLASELINNSGLGKLDQVQ
                     LQKRLTGKIVSVSPYIRELYEGLNGNASPEDLELMFQLIYLYFTEPRKDLEAFKTFID
                     KQKAFLKNIEANPQFYFMIESNRIKTQNHPRQQFPTVEALEKLNFENAFRIYQERFGD
                     ASDFTFLFVGNFEENHIRMLAREYLGNLPSTSRKETWKDLKILKPAGVVTKNFSRGEA
                     PKTYVDITYHGDFEWNDLNRFKLSSALDVLRIKLREALREDKGGVYGVSVNGGSALEP
                     SPKYTITISFNCDPPRTSELIQAAQDVLNKAREIGAEDKDLQKVSETQRQTRIRQLKE
                     NRFWMTQLQGSKQYRVNPANMLLESLEEKLKTLDAASIRDAMNHYLNEGNKITITMQP
                     EQKSN"
BASE COUNT         2272 a         2347 c         2451 g         2791 t
ORIGIN      
        1 cctttcgtgt aaacggggat ggggtatgat cagttttggg gtctttataa tttccctgcc
       61 ataaaagagg atgtccaaat caatgatccg gggctcccag atgccctggc gaatccgccc
      121 catgaaagat tctatctgaa gcaggagctc caggatccgg cttgcaggcc gggtggacgc
      181 gatacaaacg acctggttaa aataatcggg ttgttcaaga tggccccagg cctgggtctt
      241 gtatatcgag gaagacacct gtatgcccgc gatcctgctt tcaataaggt ggcaggcttt
      301 tttcaattgc cggaccggct gatttaggtt gctgcccagg gacaggaaaa cttgttttgg
      361 agtggccatt ggtggaccac aaaaatagac caaactcccg cttctcccat ataaaaacgg
      421 gagaatgttc aacttgggcg gttcagttca ggcccagcct tttcaggtgc gctttatgta
      481 cccaatacac aataaacaga aaaagcgcca acaggtatat ttgggcgaaa ctcacatatc
      541 cgttgagcgg atgaagtaca cgctgaaaca tgcccgggat attgctgaag aggatgatgc
      601 caagcaatac gggggtcaca aatttgatga tgtacttata gaccaccggt acccggatgt
      661 ccgcaccgcg ggtaatttcc tcccaggccc tgtcgatgcc aaaaatccag gcgaaaagta
      721 caatttccgc gaaggcgaaa atgaccaggg aataggtacc ggcccagtca tcgtattcgg
      781 taaagacgcc ttccttgtaa aagatgacgg taggcatgcc catgatgagg gtgatcaatc
      841 cgaaaatcca ggcgccccgg gaaaaaggga tattaaattc atccttgaga aagccgatga
      901 ccggggttcc catggccagg gaagaggtga cgcctgcaaa aaacaacaac ccaaaccaca
      961 tcattcctgc aaaagtggcc aaaaggggcc cccattgctg gaacaggtag ggcagcgttt
     1021 tgaaaccaag gccataaccg ccgaggtttc ggacgatgtc cgtcatgccg tctatcccca
     1081 ggtaacccac ggccatcggg atgatgatcg cactgcccag tacaatttcc acgaattcat
     1141 tcatccagcc ggcggacatg gcattcaggg cagtatcgtc gtcctttttc agataagagg
     1201 aaaaacactg gatggttccc attcccacgg ccagggtaaa aaatatttgt ccggcagcgg
     1261 caatccaaac ggagggtttc cagatgcttg aaaaatcagg ggtccataaa taccggagtc
     1321 cttccgttcc ggcataaacg gccccttttt cacccggctg cagactcaag gtctgaatgg
     1381 ccaggagcat ggcgaaaaga attaggaggg gcattccgat cttagccact ttttcgactc
     1441 cccgctcgag ccctctggac agaatgtaga cattgagtgc caggcacagg atgtaaaaaa
     1501 cgaccggttc gtaaggaaga cccagggtgg attggcccag gtccacatac acatcaaaaa
     1561 aactgctgac cccctcctgg gacatgcccc tgaaggtacc ggtgatggag tggaatacat
     1621 aggacatggt ccaggattcg atataacaat agtaggcggc gacggcgata ttggtaaaaa
     1681 tcccgaatac cccgatgtat tttacccagc gggctgcgtg gaccctttgg aggataaagg
     1741 gaacgctgtg atcgcccatg ggacggttgc gataaggggt tccgccaaat ctgcccatgg
     1801 accattccac aaacaaaagc ggtatgccaa tcagtaggaa acacaccaga taagggatga
     1861 taaaggcgcc ccctccgtta ttgatcgcct ggaccgggaa tctcaggaaa tttccaaggc
     1921 ccaccgcatt gccagccatg gccaggacca ggcctatcct ggaaccccag cccgtatccg
     1981 acttgatggt ctttccggga atggatacta ctctaaaaaa aaaataaacc gtggcgatca
     2041 aaacgaccag gctcaccatg gaatataata tcgttgcggt caggttcatt gagcttcgga
     2101 gattaggcgg tgaaaatatg caattgcaga tagaataccc agccctgaac cggaggggac
     2161 caaagcaaat ggacaccccg ggaaatcggt ggaaatggga gtgcgaaaga ggaaaagaaa
     2221 aaaaagccgg taaaaaatac cggctcccca ttgaattact aaaactaaga ttacaccgat
     2281 ttctcagtgc aatacaaaga taatacatat tacattattt cataatgtgt aattaattat
     2341 tttttaaaaa aaaatgagtt aatatttatt ataatgttta tatcaatgat ttacaattat
     2401 atataaatat ttatttaaaa ttattgtaat catcgcaaaa aaaaatattt acatatttga
     2461 attatatata atgtgtttta tatttgatac gtgtaagttt tggttttaat tgtaaaattc
     2521 gtttttttca tgaaagccta tgaaaagtca taggcttttt gattttatac caattttatt
     2581 ggggaataaa ccctggaata ccctgtcaag ggtccaacct gttgatcaaa ccattctttt
     2641 tctgcagatc cggcaactcc tattttcctg caatcaattg atcctgaaag gctttcagct
     2701 ttttccactt gcctctggct gcaagttttg cctgggctgg ccaatgggtg ggatccgcca
     2761 gattgaattt attgcctgcc tgttccagaa taagctgcaa ggtttttacc cttgtggccg
     2821 atagcgcttg ccaggttctg atatcttctt tccgaagcaa tgcggcgatt ttgggaccga
     2881 tgccttcgat tatttgcaaa tcatcctgtt tgatcttcct gttgaatact tcagaggcgg
     2941 cctcgaagtc ggcaggggtc aggacgggaa gtttcttttt cttaggcttc tttagccggc
     3001 cggcatataa tttttcctgg agttttttaa gtttttgcca atccccggat gcagccagcc
     3061 tggcctgctc cggccaggtc gtcgggtcgg ccagggtgaa ggaggtcccg ccttttccta
     3121 ggatggcctg gatatcttcc actttttgct ctgacagtgc agaccaggat ctcacctggt
     3181 tgcggcgaag taagttggaa attttaggtc cgattccttc gatgattttc agatcatccg
     3241 gctttatctt tttacccata agggaggaaa ccgctctcca gtccacaccg gttggttcgg
     3301 gcccgggctc tttggtttca ggctccggtt ttcggacagg aactggaacc gggctggcca
     3361 atttgacttt cagtatttcc ctttcatttg aaatatcctt gatttcctgg ttaaaacggg
     3421 cttcaattcc tgcaattcgt tcggcatgtt ctttttcaag gtcttgcagc cttttttgcc
     3481 aggcccggga ctcctggttg tatttcagct ggtattcctt tgccttttcg atgtcttcct
     3541 ccaggtgtga gttttgccgc tggctgtcgg ccagttgcaa ttgaacgatg ttgaatttgt
     3601 cactgagctc tttaaaccgt ttttctgcct cccgcaggga ttctccattc ctgtcgcgtt
     3661 ccacgagggt ttcattgaga ttattctgga cggttgcgag ttcggactga aggccgagaa
     3721 atcggtcttc ccattccccg atggaggccg cttcggtctc tttggcccgg agatgctcgt
     3781 tgagttgctc ctgggcatgt tgcaggtcga ccctggcatc gtaatatttc ctttcccact
     3841 cggcaatgat ggcttccagg gtgttttgat gcttgaggga ctgggaatgt tgcaactgtg
     3901 tggcttccag cgcatcctgc agttctttga acctcgtttc ccaggtgatc agtgaatttg
     3961 cctggctatt gctggtctgc aggctggacg agagttgttg ttcggtttga tgcagctcct
     4021 tctgtacgga atgatatttt ttttcccact ctgaaataag ggtttccagc acactttgat
     4081 tcgttttgtt ttctgccaat tgagcccggg cggcatccaa agcctgttgg gttgaattca
     4141 acttttgttc ccagtccccc gtttgtgaag cctgatcctg gtattgccgc ttcgagtgga
     4201 ccagttcctg ctggctctgc tccagttttt gccggagttc gcgcaggact tgttgttgtt
     4261 gctgtacgga ttgttcataa ttctccaatt gcaactggct ggctttcagt ttttgctcat
     4321 attcttcctg ctgcgcttcg aattcctcgc gggaaatctt cagttcggca aggacttgtt
     4381 ctttttgagc aagttcctgc cggatcgagt ttagttcctc attggcagag gcgtaggctt
     4441 tttcaaaatc ctgcccgcgc tgaagttttt caataaaatc ctgctgccgg gtagtccatt
     4501 cggcatcttc tttcaggcgc tcctggcttg ctgtacggag ctgatccagt tcgttttcca
     4561 gctcggtgat ccgtccggac tgattatcct tttctttcag aagactggcg tgggccgcgc
     4621 tgatatgttc cgcatccatg gcggcttcct tccaccgggc ttcccattcc tgtacctttc
     4681 ggttggattc aaccagggct ttttcgaggt ctgacgattt ttgagaaagt tgagccaatt
     4741 ggttgggctc cgatttcaag gcgctgagtt ccagctccag ttgatgatgc tgaacctggt
     4801 attcctgaag acgccgctcc gtattttgtt cttcattgcg gaccctggcc agttcggatt
     4861 taagttcaga gatttcctgg agcagttggt ctgtttcccc gggttttggg gtagtttcct
     4921 ctttagtggc ctgttgttga gccaagaggc ccagttcctg ttctttactg gcaagcaaat
     4981 cttggaaatg cgccgtggtt tccttgtttt tttgctgggc gtctgtgagc gcctcggaca
     5041 gttcccggat ttgtttgtcc cggttttcca attcatccct gaggttactg atggtatcct
     5101 cctgaagctt gtgatcggag gcttgctggt ttaaccggtt ggcgagttga ctgatttctt
     5161 gctccagccg gtctgtggcc gatacagatt gggacagatc ggcttctgcc tgtttccaac
     5221 gggcctggct ttcatcggcc tttttcgagg cgagctgcaa ttggttttcg accgaggcta
     5281 aggaggaaag gagccctttg cgttcagcgg tgagggcatt gcttttttcc tctgcagcct
     5341 tgaggctttc ttcaaaacgg ttttgcagtg agcttagttc ggaaggggag acatattctt
     5401 taaattggtt ggtgagggtg tcgatttcat tctgaaggga tgagaggtta tgctggaaac
     5461 ctgctctttc ggaggcccaa tggtcctggc tggcttttga catacggatc tcctcatcct
     5521 gcattttgat cctttccctc agggaccgga tctgatcttc atattccttt ttgtcgacgg
     5581 gatctttttt ctgaccgaac caccggttcc acaaataccc aaggagccag gcgatgccca
     5641 aggccaggag gatctctccg gtatgggtca aacaggtctt ccaaaacata agcggtattt
     5701 tttatagttt ctgaatgatt tcaatttcgg tccgccggtt caggcggcgg ccttcctccg
     5761 tatcgttgcc ggcaatcggg tccgattctc ctttggaaaa gatccggatg cgggaagctt
     5821 cgatgccctt tccgaccagg aagtttttga cgcttccggc ccttgccaat cccagcctgg
     5881 tatttagttt ttccggtcct tggtcatcgg tatgtccggt gatttcaaaa accgatcgcg
     5941 cattccggtg ttcgttggct aagtttgaaa gataagtttc aatttcaggg tctttaatcc
     6001 tctggttgga attggattgg aaataaatca ggatctttcc ttcggtttca acaatttttc
     6061 cgggagcggg ctttatgttc catgatgagg ctctgaacgg ataaagatcc atgccttcgc
     6121 gcttgtcgat gcggagactg gtcctgctaa tcctcgggcg gatggaatcc ggaaacaaaa
     6181 agctcagggc ggcggccctg gccaagccca ggttggcaaa tccggtgggg ttgggttctt
     6241 catccagaaa atgaccctct atttcaagga cctttgccgg ggccatggcc aggataaggg
     6301 aatcccggaa ggccggccat ccgggctgga tgagggcgat gctgtccccg tgattaaaaa
     6361 gcaccactcc ctgttcctgg gcgggggcgg caatcaccgg tacttccttc cgggcgcaca
     6421 ggcaggtagt acatatttta caggtgtacc accaccacca gaaagccagc cagaggaatg
     6481 ttgccagcag gattaatgtt tttcgcatga tgtccttata tcttcatccc ttttttaatt
     6541 ataaactaat atcaggagaa tttgttaata tttcccaatt aaagtcatga gcgcttatcc
     6601 ccattcgata ttctggttta ggagggacct gagactgaat gacaatcacg ccctgtacca
     6661 ggcattgagt caaagcgacc aggtcctgcc tgtttttatc ttcgatacac atatcctgaa
     6721 ggatctccgc gatcctcagg ataaaagagt gcaatttata taccaagaac tttgcggcat
     6781 caacaaaaag ctggagaaaa gggcttgtgc cgtccgatta tattttgggg agccggtaaa
     6841 aatcctggaa aaactgtgta cgcagttcca cattcatgcc gtttttgcca atgaagatta
     6901 tgagccgtac ggacggacaa gggaccggga cgtggccggc atgctgaaca aaaaaaagat
     6961 tcccttccat cttttccagg accatgtgat ctgcaggccg gatgaaatcc tgaagcacga
     7021 cggcaaaccc tatacgattt acacgcccta catgaacaaa tggagagaag gggtggggag
     7081 taaaaccata gcctcctatc caagtgaaaa attattaaat aaggttaccc ctggaattca
     7141 aaccccgact ttgtccctgg aggccatcgg attccaaagc attgattgca gatacccgga
     7201 ccgcaggata tcgggcgaac ggttgcggtc ctatgccagg acccgggacc ggattccgga
     7261 cgaatccggc accagccacc tcggcataca tcttcggttc gggaccatca gtatacggga
     7321 actgttccag caggccttga gtttccgggc ggaagttttt atcaatgaat tgatctggag
     7381 agaatttttt atccagatcc tttttcactt tccccgggtg gtccattcct cctttaaacc
     7441 tcaatatgag ggcattccct ggcgcaatga tcccggcgat ttcaaattgt ggtgccaggg
     7501 tcagaccggt tatgccctgg tcgatgccgg gatgaaacat ttgaataaga ccggacagat
     7561 gcacaaccgg gtcagaatgc tggtcgccag ttttctgtgt aaacacctcc tgatcgactg
     7621 gcgttgggga gaagcttatt ttgcggaaaa gttgctggat tacgaattgg catccaacaa
     7681 cgggaattgg cagtgggcgg caggttccgg atgcgatgcc gctccttatt tcagaatttt
     7741 taatccggaa gaacaaagaa aaaaattcga ccctcagtcc gcttatgtcc tgaaatgggc
     7801 gcctgaatac ggggaattca accagccggt ccctatggtg gaccaccgtt ttgcacgcga
     7861 aagagccttg atggtttata aaagaacctt ggaagaatcc aggcaggggc taaaataaca
     7921 aagtggccac gctccttgag gtgaccacct tgcttgaacc aacttatgta ccaaaatgct
     7981 tctttaactt tccctcctgt tgggaaatgg attatatcta tttattatcc ccggcacacc
     8041 ggggttaaag ggccatttta tttgtaatgt ttcaatgcat tggtttctct ttcgtgcctt
     8101 tgcattatgt atctttaaca ccgccttaac tgcgtttatt gcgaaccagg taaatttttt
     8161 tataattttt ttacactcct ggtccttcaa tagatccagc aatccattgt ttgcagattt
     8221 tcaattagtt acaaagggtt ccaagccgtc cggccatcta ttttttaaaa tcggagcctt
     8281 ccccaaccat tgaggcaggc cggagaagga aggcgaatat caattgcttt tctgttccgg
     8341 ctgcatggta atggtaatct tattcccttc attcaggtaa tggttcatgg cgtcacggat
     8401 cgaagcggca tccagtgttt tgagcttttc ttccagggat tcaagaagca tattcgccgg
     8461 attgacccgg tattgttttg aaccctgcag ctgggtcatc cagaacctgt tttcttttag
     8521 ttgcctgatc cttgtctggc gctgggtttc ggacactttc tgaaggtcct tgtcctccgc
     8581 cccgatctcc cgggccttat tgagtacatc ctgagcggcc tggatcagtt cagaggtccg
     8641 gggagggtcg cagttgaaag aaatggtgat ggtgtatttt ggacttggtt ccagggcgga
     8701 gccgccattc acggacaccc catatactcc ccctttgtct tcccggagtg cttcccggag
     8761 tttgatccgc agaacatcca gggcggagct tagtttaaac cggttgaggt cgttccattc
     8821 aaaatcgccg tggtaagtga tgtccacata agtctttggc gcttcacccc tggaaaagtt
     8881 cttggttacc accccggccg gtttgaggat tttgaggtct ttccaggtct ccttgcggct
     8941 ggtggaaggc aagttgccca ggtattccct tgcaagcata cggatatggt tttcctcaaa
     9001 atttccaaca aacaaaaagg tgaaatcact ggcatctcca aaacgttcct ggtagatgcg
     9061 gaaggcattt tcgaaattca atttttccaa cgcttccacg gtggggaatt gctggcgcgg
     9121 atgattttgc gttttgatcc ggttgctctc aatcataaaa taaaactggg gattggcctc
     9181 aatgtttttg aggaaggctt tttgtttatc gataaaggtc ttaaaagctt ccaggtcttt
     9241 tcgcggttct gtaaagtaaa ggtagatgag ttgaaacatc agctctaagt cttccgggga
     9301 ggcgttcccg ttcaagcctt catacaattc ccggatataa ggcgaaacgc ttacaatttt
     9361 tccggtcagc cttttctgca attgtacctg gtccagttta ccgagtccgg aattgttgat
     9421 caactcggag gcaaggctcg catccacatc ttcctcctcc cggtagaggg aggttcctcc
     9481 ggggctgtaa gcggccatga ggatttcatc gttcttaaag tccgtctttt tcaggatgac
     9541 ggtgacgccg tttttaagga taaactcggt cgtgcccagt ttttcatcct ttttttccga
     9601 aatgatttcc actgcggtaa aatccctgct gatcaggggc tcatcgctga cctggtcttc
     9661 atagggccct atttccatgc cttttatttc ggttgccatt ttgaggatgg tttcttcggt
     9721 ggggtaggag aggcttggtt tgtccgggcc ggtaatgatg atcactcttc cctcgtcccg
     9781 gatccatttt ttaggcaaca gatttatttc cagcagatca atcgtcggca atagtttctt
     9841 tgccagttca agttcttgtt c
//
LOCUS       DAOQXT010000144         9497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_7709_length_9497_cov_5.796971, whole genome shotgun sequence.
ACCESSION   DAOQXT010000144 DAOQXT010000000
VERSION     DAOQXT010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7709_length_9497_cov_5.796971"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKM27_17620"
     CDS             <1..664
                     /locus_tag="PKM27_17620"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNR09144.1"
                     /translation="KKLIRYLVRSHTYMQSAAVSADQLQKDPGNIWLSHAPRPRLSAE
                     VIRDQALAVSGLLSEKMYGPSVMPYQPEGIWKTVYSNAKWIESKGEDRYRRAIYTYIR
                     RTSPYPSMITFDAPSREFCVNRRIATNTPLQAMVTLNDPVYVEAANHLADLMDQHPGS
                     LEDKIGFGFEKAVFHKISSEALRTLVKLYNEPSPEAGGSITPMSQVAIAILNLDQFLT
                     KS"
     gene            668..2143
                     /locus_tag="PKM27_17625"
     CDS             668..2143
                     /locus_tag="PKM27_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNR09145.1"
                     /translation="MKGTELFTELRIRQLQMQTRRHFLQKCTTGLGALALGSLTGCMN
                     RNVPGHGKNPFATKSPPGFAKAKRVIYLHMAGAPSQLELFDYKPELAKLDGKPCPGSL
                     LEGRRFAFIQGVPKMLGPQYRFKPCGQSGTYLSELLPHFSNVVDDACVIKSMHTDEFN
                     HAPAQLFMHTGHGRLGRPSMGSWVSYGLGTENQDLPGFIVLVSGGKTPDAGKSVWGSG
                     FLPSVYQGVQCRSDGEPVLYLNDPKGMSRDQKGKIIEAINKINEESYKTFGDPETLTR
                     IEQFELAYRMQASVPEAMDITREPASMHALYGTTPGQSTFANNALLARRLAERGVRFI
                     QLFHWGWDAHGAGREEALNMGFKDRCKEVDRAMSALILDLKQRGLLEETLVVWGGEFG
                     RTPMRENRDGKVMTWIGRDHHTECFSIWMAGGGIRGGMTYGETDDLGYFGIQDRVHVN
                     DLHATMLHCLGIDHEKLTYSFQGRPFRLTDTGGKVLDKILA"
     gene            2168..4264
                     /locus_tag="PKM27_17630"
     CDS             2168..4264
                     /locus_tag="PKM27_17630"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HNR09146.1"
                     /translation="MFEKFLQILGHFHPLLVHIPIGTLVLTYILYRFNGHGNRRNMRH
                     AISTGLFISTGAAVLSAICGLLLARQGGYDEDLLSWHKYLGFVVVGFSLILWYIHQDI
                     YKRDKTFTSTAFRWLFNLCMIALVVGGHLGGSLTHGRGYLFKTEARAVNSPPIDPSTL
                     SQPDIGQRKVYEEIIQPILKDKCTSCHNPDKKKGDLILTDSKGLLAGGKNGPVVKMGD
                     PESSALYTNLQKPLEDDTHMPPDGKRQLTETEKELIAWWIKNHTSFDDKVADFPAFEG
                     KLAIISSSPGQNQFDFDLAAIRPADQKIIRNLQEDGYKIFSLAQDIPLLDVSLAFNKK
                     MDRKTLGQLKRVQGQVYKLDLSHTGLTDDLASGIPDLPYLEHLNLGNTAIGDASLDEL
                     DRFPRLKKLNLFQTNVGDAGLEKLKSLASLKQLYLWKTKTTPEGIEALQSAMPGVEIV
                     QGINMDTLVKMKLPLPGIRTETNLFNDSIVVEMTCFFKDAALHYTLDGSDPGLQSPRY
                     MQPLVLKEGADIRLIAFKDGWESSDTATLQVRKAGLKIKEVSLLKDPAKRYQADGPGT
                     LADLNLGSDPGDGKWLAYQGEDFIATLQLEALRPVSKVKINTIQNHGLWVFYPKSIEV
                     ELSADGQTFQPVAKNNYGSPQRADKGTTKLIEVAFPPAQAKYVKVILRNQGKNPSWHP
                     QRGKPSWIFCDEMMVE"
     gene            complement(4265..5437)
                     /locus_tag="PKM27_17635"
     CDS             complement(4265..5437)
                     /locus_tag="PKM27_17635"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNR09147.1"
                     /translation="MLYYLLRPLVRITLRIFFSKIYLHHLDRIPLNRPVLIASNHPSA
                     FLEACLLATHFPKSLHFLVRGDIFINRFVVALLEQLHLTPIYRFVDGFKNLRSNDATF
                     NACYDRLSNKEIIVVYAEGNTTQEKRLRPIQKGLARIAFGAMDKYPDMDLCVVPLGVN
                     YTHPNDFRSEVFVETGLPIELKEYYLRYKEDNNKAVQELTQAVEESMKPLVIHVEKNE
                     DLELAEKLWRISKAQANPPYLPVVELAGERFAKDKMISERVNHLTEEEKSSLNQTLDP
                     AAAKPVRHAESRSFAIPALLGKYINYPPFMIAMGIAASKVKRIEFYASVLIGLGMFLY
                     LFYMLAVFILFRAIGWSGLLGLAILWVSGICYLYYLYLKDREVNLALRETYPELPV"
     gene            5497..6402
                     /gene="miaA"
                     /locus_tag="PKM27_17640"
     CDS             5497..6402
                     /gene="miaA"
                     /locus_tag="PKM27_17640"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNR09148.1"
                     /translation="MIRKYLIVIAGPTAAGKSKLAVDLALHYRCEILSADSRQVYREL
                     NIGVDKPTPRQMAAVPHHLIGQVSIHETYNAGIYEKEALRILEELYTRMDLAILVGGT
                     GLYINAVLEGFDVFPEISPGISLRLKKTLEENGPEALQEELKQKDPEYYARVDIHNTH
                     RLIRALEVIESAGLPYSQLRQKRKKTRPFIPVPILLTAERSRIYQRINQRVDEMVKLG
                     LKDEALSLYPFRHLNSLQTVGYQEWFDHFEGKLTEAEAIDKIKQHTRNYAKRQWTWFR
                     RTGWPEFRAEESSKIIAYLDTMIKK"
     gene            6501..6929
                     /locus_tag="PKM27_17645"
     CDS             6501..6929
                     /locus_tag="PKM27_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNR09149.1"
                     /translation="MPDLFKYRPTLEQLNERFENTLAQTLGIEIIELGEDFIRATMPV
                     DHRTHQPMGLLHGGASAALAETLGSIGSLLCSDHPHAHPVGIEINANHLKSVTSGKVT
                     GKASPVKIGRNIHVWNIEIRNDKHELTCVSRLTVMMVTKP"
     gene            6963..9335
                     /locus_tag="PKM27_17650"
     CDS             6963..9335
                     /locus_tag="PKM27_17650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HNR09150.1"
                     /translation="MFWFGSMQAQDYNTRLLSNVRFTENCANIWGFVDQSGREYAIIG
                     RTSGTSIFDVTDGAKPEFLVHIPGAQGQWREIKSWQNQVYVVADQGQDGLLIIDMTEA
                     PHAINWRFDRRQFQVVDETLNINKTDFLHRAHSLFIDEKGFCYINGSNLHPGIVIFKL
                     NPGLQTPEYMGVINNNYCHDSYARNDTLWSADILAGEFTIWDLKDRTKPKKLGQQRTG
                     SAFTHNIWLSDDGKYAFTTDERANAFVEAYRVDDLSNIVRTDRYRSRTTQIRGTIPHN
                     THYHMGYLVTSYYTDGFTIVDATDPSHLVEVGAFDTYPAGEGDFHGCWGVYPYLPSGN
                     ILASDIEFGLYVVKPTYKRASYLRGTVRDSISGGPLANVKIRMGLAPFNEVLSAPDGS
                     FKTGGPYTGKVTIRLSKEGYLTKEVEVDLQTGQLTQTDLVLMPLNTHILNIRVIDSIT
                     GQSIPGASIRFVSEESSFDVETDGAGRTSQLMYEGKWTLGVAIWGFRFKTRSLILNGQ
                     TGEIDIPLTRGYEDNFLFNLNWTAQSDLFRGVWGRAEPRGAYIREEVINPEYDLTDDF
                     GDQCMITGNGSTSATGDDVDNGMTRLISPAMDLSAYKAPVIEFYAWTVRRNLTESVPG
                     VGSHKVYLAAGQDTILLLTLEPNMPAWTKYRIDLNPSLVKNKPCRLIFEASEPVVVDS
                     RNILEFGLDGFLVLENNSTPVRPEQPQPLDWKAFPSPFSHRITLSGPEKPFDRKVEVI
                     SVLGTKMLTLTWPKFLPAFQIDPGLPSGTYIFRLISPGKADEVLKVIRSD"
BASE COUNT         2422 a         2559 c         2395 g         2121 t
ORIGIN      
        1 aaaaaaactg atccgttatt tggtccgttc ccacacctat atgcaatccg ccgcggtgag
       61 cgcagatcaa cttcaaaagg atccgggcaa tatctggctg tcccatgctc cccggcccag
      121 gctcagcgct gaagtcatcc gggaccaggc cctcgcggta tccggccttt tatcggagaa
      181 aatgtatggt cccagcgtga tgccctatca accggaaggg atctggaaaa cggtgtacag
      241 caatgcaaaa tggattgaaa gcaaaggtga agacaggtac cggagggcta tatacaccta
      301 tatccggagg acgagcccct atccatccat gattaccttt gacgctccca gccgggaatt
      361 ttgcgtcaac cgcaggatcg ccaccaacac ccccctgcag gccatggtca ccctgaatga
      421 cccggtttat gtcgaagcgg ccaaccacct tgcggacctg atggatcaac atccgggcag
      481 cctggaggac aaaatcggtt ttggatttga aaaagccgtt tttcataaaa tttcctccga
      541 agccttgcgg acattagtga agctttataa tgagccttct ccggaagccg ggggatcgat
      601 aacccccatg agccaggtcg ccatcgccat tttgaacctt gaccaatttc ttaccaaaag
      661 ctgatcaatg aaaggaacgg aattattcac cgaactgagg atccggcaac tgcaaatgca
      721 gacccgtcgc cactttctgc agaaatgcac caccggcctg ggggccctcg ccctgggttc
      781 tttgaccgga tgcatgaacc ggaacgtacc cggtcatgga aaaaatccct tcgccacaaa
      841 aagccctccc ggttttgcca aagccaagcg ggtcatttat ctccatatgg ccggagcgcc
      901 ttcccagctt gaactgtttg attataaacc cgaactggcc aaactggatg ggaaaccctg
      961 ccccggctcc ctgctggaag gacgtcggtt tgcctttatc cagggggtac ccaaaatgct
     1021 gggcccccaa taccggttta agccttgcgg ccagtccggc acctacctgt ctgaactgtt
     1081 gccccatttt tccaatgtag tggacgacgc ctgtgtaatc aagtccatgc ataccgacga
     1141 atttaatcat gcccctgctc agttgtttat gcatactggg cacggaagac tgggaaggcc
     1201 cagcatggga tcctgggtat cctatggtct ggggaccgaa aaccaggacc ttccgggttt
     1261 tatcgtactc gtctcagggg gcaaaacacc ggatgcgggt aaaagcgttt ggggcagcgg
     1321 atttttacct tctgtttatc agggggtgca gtgccgcagc gacggggaac cggtactcta
     1381 cctgaatgat ccaaaaggga tgagccggga ccaaaaagga aagatcattg aggccataaa
     1441 taaaataaat gaagaaagtt ataaaacgtt tggagacccg gaaaccctta cccggataga
     1501 acagtttgaa ctggcatacc ggatgcaggc ctctgtgccc gaagccatgg acattaccag
     1561 ggaacccgcc tccatgcacg ctttgtacgg caccactcca ggccagtcca cttttgccaa
     1621 caacgccctc ctggcccgca gattggccga aaggggcgtc cgttttatcc agttgtttca
     1681 ctggggctgg gatgctcacg gcgcagggag ggaggaagcc ctcaatatgg gattcaaaga
     1741 ccggtgcaaa gaagtggatc gggccatgtc tgccttgatc ctcgacctca aacaaagagg
     1801 tttgcttgaa gaaaccctgg tggtctgggg cggtgaattc ggccgtaccc caatgcgcga
     1861 gaaccgggat ggaaaagtaa tgacctggat tggaagggac catcacactg aatgtttttc
     1921 catctggatg gccggaggcg gcatacgcgg gggtatgacc tacggggaaa ccgatgacct
     1981 gggatatttc ggcatccagg accgggtcca tgtcaacgat ttacacgcga ccatgctgca
     2041 ttgcctgggc atcgatcatg aaaaactaac ttactctttc cagggaaggc ccttccggct
     2101 gaccgacaca ggcggaaaag tattggacaa aatcctggct tgacttccct ttaaccgcta
     2161 tcgacttatg tttgaaaaat ttttgcagat cctggggcat tttcatcccc tcctggtcca
     2221 catcccgatc ggcacactgg ttctcaccta tattctgtac cggtttaatg gtcacggaaa
     2281 ccgtcggaat atgcggcacg ccatcagcac cggccttttt atttctaccg gagccgcggt
     2341 cctctcggcg atctgcgggc tcttgctggc caggcaggga ggatatgacg aagacctcct
     2401 ctcctggcat aaatacctcg ggttcgtggt cgtcggcttc agcctgatcc tttggtacat
     2461 ccaccaggat atttacaaac gggacaagac cttcacctcg accgccttcc ggtggctgtt
     2521 taatttatgt atgatcgcac tggtggttgg cggtcacctc ggcggcagcc taacccatgg
     2581 cagaggttac ctgtttaaaa cagaagcccg tgcggtaaac agccctccca tcgatccttc
     2641 caccctcagt caacccgata ttggccaacg aaaagtgtat gaggaaatca ttcaacccat
     2701 cctgaaggac aagtgcacct cctgccataa cccggataaa aagaagggag atctgatcct
     2761 caccgattcc aagggacttc ttgcgggcgg gaaaaacggg ccggtggtca aaatgggcga
     2821 tccggaatca tccgccctgt acaccaacct tcaaaaacca ttggaagacg atacccatat
     2881 gccccccgat ggcaaacggc agctcaccga aacagaaaaa gaactcatcg cctggtggat
     2941 aaaaaaccat accagctttg atgataaagt tgccgatttc ccggcctttg aaggcaagct
     3001 cgccatcatc tcctccagcc cgggtcaaaa tcagtttgat tttgacctgg ccgccattcg
     3061 ccccgcggat cagaaaatca tccggaacct gcaggaagac ggttataaaa tattttccct
     3121 tgcccaggac attcccctgc tggatgtttc cctggctttc aataaaaaaa tggataggaa
     3181 aacccttggc cagttgaaac gggtccaggg ccaggtgtat aaactggacc tctcccatac
     3241 cgggctgacg gatgacctgg cgtccggcat tccggacctg ccttacctgg aacaccttaa
     3301 cctagggaat accgccatcg gtgatgcctc cctggatgag ctggaccgtt ttccgcggct
     3361 gaagaagctg aacctgttcc agaccaatgt cggggatgcc gggcttgaaa aattaaaatc
     3421 cctggcttcc ctgaagcagt tatatctctg gaaaacaaaa accaccccgg agggaatcga
     3481 agcccttcaa tccgccatgc cgggtgtgga aatcgtccag ggaatcaata tggacaccct
     3541 ggtaaaaatg aaacttccct tgccgggtat ccgtactgaa accaacctgt tcaacgacag
     3601 tattgtggtg gaaatgacct gcttttttaa agatgccgcc ttgcattata ccctggatgg
     3661 ttccgatccg gggctccaaa gtcccagata tatgcagccc cttgtactga aggaaggagc
     3721 ggatatccgg ctgatcgcct ttaaagacgg atgggaatcc agcgataccg caacccttca
     3781 agtccggaag gctggcctga aaatcaaaga ggtgagcctg ctaaaagatc ctgccaaacg
     3841 ctaccaggcc gatgggccgg gtaccctggc cgatctcaac ctgggttcgg atcccgggga
     3901 tggaaaatgg ctggcttacc agggagagga ttttattgca acccttcaac tggaggcgct
     3961 gcgccccgta tccaaagtca agataaacac cattcaaaac cacggattat gggtttttta
     4021 tcccaaatcc attgaagtgg aactgtccgc cgatggccaa acctttcaac cggtggccaa
     4081 aaacaattat ggttcaccgc agagagcgga caagggtacg accaagctga tagaagttgc
     4141 tttcccgccc gcccaggcga aatatgtcaa agtcattttg cgcaaccagg gaaaaaatcc
     4201 ttcctggcat ccccagcggg gcaaaccgag ctggatattt tgtgatgaga tgatggtgga
     4261 atgatcaaac gggcaattcc ggataagttt cccgcaatgc cagattgacc tcccggtctt
     4321 tcaggtacag gtaatacagg taacaaatcc cggagaccca caggatggcc aatcccagga
     4381 gaccagacca tccgatggcc cggaacaaaa taaaaaccgc cagcatataa aacaaataca
     4441 gaaacatgcc caggcctatg agcacggagg cataaaattc aattcttttt accttgcttg
     4501 ccgcgatgcc catggcgatc ataaacggag gatagttgat gtatttgccc agcagggccg
     4561 gtatggcaaa cgatcgcgat tcggcatggc ggacaggctt tgcagcggca ggatcaagcg
     4621 tttgattcaa gctggatttt tcttcttccg ttaaatgatt tacccgttca ctgatcattt
     4681 tgtcttttgc aaaacgctct ccggccaatt ccaccaccgg taaatagggc gggtttgcct
     4741 gtgctttact gatacgccac aatttttctg cgagctccag gtcttcgttt ttctctacat
     4801 ggatgaccag gggtttcatg ctttcttcga cggcctgggt cagttcctgg accgccttat
     4861 tgttatcttc cttgtatctc aggtaatatt ctttaagctc gataggcaat ccggtttcca
     4921 caaatacctc gctccgaaaa tcattcgggt gggtgtaatt caccccgaga ggcaccacgc
     4981 acaggtccat atccggatat ttatccattg ccccgaaagc aatccgggcc aggcccttct
     5041 ggatgggccg gaggcgcttt tcctgggtgg tatttccttc cgcatagacc acaatgatct
     5101 ccttgttgct taatcggtcg tagcaggcat tgaaggttgc atcattggac ctcaggtttt
     5161 taaatccatc gacaaatcga taaatggggg tcaggtgcaa ttgttccagg agggcaacca
     5221 caaaccggtt gataaaaatg tccccgcgga ccaggaaatg cagcgatttg gggaaatggg
     5281 tggccaggag gcaggcttcc aggaaagcgg agggatggtt gcttgcgatc aggacaggcc
     5341 ggttgagggg gatccggtcc aggtgatgca ggtagatttt gctaaaaaaa atcctcaggg
     5401 tgatcctgac caggggccgg agaaggtaat acagcatgta aggttggcta gaaagcgatc
     5461 aaaaatatta ctttcacgca aattctgcgc aatcttttga tcaggaagta cctcatcgtc
     5521 atcgcaggcc ccactgccgc gggcaaatca aaacttgcgg tggatctggc cctgcattac
     5581 cgatgtgaga tcctgtccgc cgacagccgc caggtttacc gggagttaaa catcggagtg
     5641 gacaaaccca ccccccggca aatggccgct gtaccccacc atttaatcgg ccaggtctcc
     5701 attcacgaaa cttacaatgc cggcatctat gaaaaagagg ccctcaggat attggaggag
     5761 ctctataccc gtatggacct cgccattctc gtgggaggca ccggcttgta catcaacgcg
     5821 gtgcttgaag gatttgacgt gtttcccgag atcagccccg ggattagcct ccggctgaaa
     5881 aaaacgctgg aggaaaatgg cccggaagcc ctgcaggaag aattaaaaca aaaggacccg
     5941 gagtattacg cccgggtcga cattcacaat acgcatcggc tgatccgcgc cctggaggtc
     6001 attgaaagcg ccggactgcc ctattctcaa ctcaggcaaa aaaggaaaaa aaccaggccc
     6061 ttcattcctg tccccatcct gctcaccgct gaacgaagcc ggatttacca aaggatcaat
     6121 caaagagtgg atgagatggt gaaactggga ctcaaggacg aagcactgtc gctgtacccg
     6181 ttccgccacc tcaacagcct gcagacggta ggctatcagg aatggttcga tcattttgaa
     6241 ggcaaactta ccgaagcgga agctatcgat aaaataaagc agcacaccag aaattatgct
     6301 aaaaggcaat ggacctggtt tcgtcgcacc ggctggcccg aattcagggc cgaagaatcc
     6361 tcgaaaatca ttgcctacct cgacactatg atcaagaagt agtccctttc tccctttctc
     6421 cctttctcct ttctcctttc tcctttcgcc cttcaccttt tacctttccc cctttacctt
     6481 tctccgtaac ttgcaccctt atgcccgacc tctttaagta ccgccctacc ctggagcaac
     6541 tcaacgaaag atttgaaaac acccttgccc aaaccctggg catagagatc atcgagctcg
     6601 gggaggattt tatccgggcc accatgcccg tggaccaccg cacccatcag cccatgggac
     6661 tgctgcacgg cggggcctcc gcggcccttg cggaaaccct cggaagcatc ggctccctgc
     6721 tttgttccga tcatcctcat gctcacccgg tcgggatcga gatcaatgcc aatcacctca
     6781 aaagcgtgac ttccggtaaa gtgaccggta aggcgagccc ggtgaagatc ggacggaata
     6841 tccatgtctg gaatatcgaa atccggaatg ataaacacga attgacctgt gtaagccgtt
     6901 taaccgtaat gatggtcacc aaaccctgag cctgccggat gagatatctt ttattgattt
     6961 gcttgttttg gtttggatcc atgcaggccc aggattacaa cacccgttta ttgtccaatg
     7021 tccggtttac tgagaattgc gccaacatct ggggattcgt ggaccagtca ggaagggaat
     7081 acgccatcat cggccggact tccgggacct ccatttttga cgtgaccgac ggcgcaaaac
     7141 cagagttcct ggttcatatc ccgggagccc aaggccaatg gagagaaatc aaaagctggc
     7201 aaaaccaggt atatgtggtg gcagaccagg ggcaggatgg tctcctgatc attgacatga
     7261 ccgaagctcc tcatgccatc aactggaggt ttgaccgcag gcagttccag gtcgtagatg
     7321 aaaccctgaa catcaataaa accgattttc tccatcgcgc ccacagcctc tttattgacg
     7381 aaaagggatt ttgttatatc aatggaagca atctgcaccc ggggatcgtc atttttaaac
     7441 tcaatccggg cctgcagacc ccggaataca tgggcgtcat caacaacaat tattgccatg
     7501 acagttatgc gcggaacgat acgctctggt ccgcggatat cctggccgga gagtttacca
     7561 tctgggacct caaggaccgg accaaaccga aaaaattggg ccagcagagg acaggcagtg
     7621 ccttcaccca taatatttgg ctttccgacg acgggaaata tgccttcacc acggatgagc
     7681 gggccaatgc ctttgtagaa gcctaccggg tcgatgatct ttccaatatc gtccggacgg
     7741 accgctaccg cagcaggacg acccagatcc gcggcaccat tccccacaat acccattacc
     7801 acatgggata cctggtcact tcctactata ccgacggctt cacgatcgtg gacgccaccg
     7861 atcccagcca cctggtggaa gtgggagcct ttgataccta ccctgcaggg gaaggggatt
     7921 tccatggctg ctggggtgta tatccctacc tgccatcggg caatatcctg gcttcagaca
     7981 ttgaattcgg actttatgtg gtgaaaccta cctataaaag agcttcttac ctgagaggca
     8041 cggtgcgcga ttccataagc ggcggcccgc ttgcgaacgt aaaaatcagg atgggcctcg
     8101 ctccgttcaa tgaagttctg agtgcaccgg acgggagctt taaaaccggc ggtccctata
     8161 ccggaaaggt gaccatacgg ttgagcaagg aaggttattt gactaaagag gtggaagtag
     8221 acctgcaaac aggccagttg acccaaaccg atctggtgct catgcccttg aatacccata
     8281 tactcaacat ccgggtcatc gattccatta ccggccagag cattcccgga gcttccatcc
     8341 gtttcgtttc ggaagaaagc agtttcgatg tggaaaccga tggagcggga cgaaccagtc
     8401 aactgatgta tgagggcaag tggactcttg gagtagccat ttggggattc cgtttcaaaa
     8461 cccgttcact gatactgaat ggccaaaccg gcgaaatcga cattccgctg acccggggtt
     8521 atgaagataa tttccttttc aacctgaact ggaccgcgca aagtgatttg ttccgtggag
     8581 tatggggcag ggccgaaccc cggggcgctt atatccggga agaggtcatc aatccggaat
     8641 acgatttgac ggacgatttt ggggaccaat gcatgatcac cggaaacggt tccacttcag
     8701 ccacaggaga tgatgtggac aatggaatga cgaggctgat atcgccggcc atggaccttt
     8761 ccgcttacaa ggctccggtc attgagtttt atgcctggac cgtccggaga aatctcacgg
     8821 agtcagtgcc tggtgtgggc agtcacaagg tttatctcgc tgcgggacag gataccatac
     8881 tcctgctgac cctggaaccg aatatgccgg cttggacgaa ataccggatt gacctgaacc
     8941 cgtcactggt aaaaaacaaa ccctgccgcc tcattttcga agcgagtgaa ccggtcgtgg
     9001 tggattcgag gaatatcctg gagtttggcc tggatggttt tttggttttg gaaaacaaca
     9061 gcacgccggt ccgtcctgaa caaccgcagc cgttagactg gaaagcattc ccatcccctt
     9121 tcagccaccg gatcaccctg agcggacctg aaaaaccgtt cgaccggaaa gtggaagtga
     9181 tctcggtact gggtacgaaa atgctcaccc tgacctggcc taaatttttg cctgcctttc
     9241 aaatagatcc gggccttcct tccggcacct atatcttccg gctgatctcc cccgggaaag
     9301 cggatgaagt actgaaggtg atcagatcgg attaaatact tctggacggg aaggaaagcc
     9361 cggtcctgag gattgcaata cccgatccac ctaaagcagg caaaccgttc agatccttaa
     9421 gaatggttac attcaattca agtttaaccc aaatcatgca atagagtttc gtaatgaaaa
     9481 ccggaaggcc aataaag
//
LOCUS       DAOQXT010000145         9488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_7722_length_9488_cov_6.351638, whole genome shotgun sequence.
ACCESSION   DAOQXT010000145 DAOQXT010000000
VERSION     DAOQXT010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7722_length_9488_cov_6.351638"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..2906
                     /locus_tag="PKM27_17655"
     CDS             282..2906
                     /locus_tag="PKM27_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNR09151.1"
                     /translation="MSKKKAPPIIPVPKGAEGIKITSLTSMYESYFLDYASYVILERA
                     VPAIQDGLKPVQRRILHALYEIDDGRYNKVANVIGHTMQYHPHGDAAIRDALVNLGQM
                     ELLIDTQGNWGDARTGASAAAPRYIEARLTKFALEVLFNPKTTGWLLSYDGRRKEPAF
                     LPSKFPILLAQGVEGIAVGLSTKILPHNFIEIIDAAVKYLEGKKFKLYPDFQTAGSID
                     VSEYEDGQRGGRVKIRAKIEAVDKKNLLITEIPFGTTTSSLIESILKANEKGKIKIKK
                     ITDNTAAEIEIAIELQPGVSPDVTIDALYAFTDCQLSVSPNACVIVDQKPRFVCVADI
                     LRHNVDSTKNLLRQELEIKRDELTEVWHFANLEKIFIENRIYHEIEECESWEEVVETI
                     HLELKKYVATPSENPGPRDKRLKLWREITDEDVARLTEIRIKRISKYNTFKADELILE
                     TEKSLEQVHYDLRHLVEYTIAWFEHLRKKYGEGKERRTTITQFENIQAHTVVANNTKL
                     YADDKEGFIGWGLKKDQSRLVGECSDIDDIVAFRKDGKMIVSRIGEKVFMGKDLKYVN
                     VWKKNEDRTVYNVIYLDGESGKAFAKRFQIGGVTRDKEYPIATDHKESRLLYLSANPN
                     GESEIVGIQLSQGCTARIKQFDFDFAGLEIQGRGSKGITVTKYPVKRVDLKEKGKSSL
                     GAQAIWLDEVTGRLNTDERGKAIGEFDTGDLILSLWNDGSYQLNPVEMSLRYNADELI
                     WIGKFEPEIPIGAVYYDGDKGWTMVKRFLIETTTTGSRFKFITEHKDSRLLYAQAGED
                     PVIEYSWKAKNQKHSREIRLSEFIDVKGWKALGNRLSEYKLTGIRPKENTAGGSAPTG
                     KNAVEFEIVNGNGQKKLF"
     gene            complement(3095..3739)
                     /locus_tag="PKM27_17660"
     CDS             complement(3095..3739)
                     /locus_tag="PKM27_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09152.1"
                     /translation="MSHFRSLDQLHQAWISWPPAQLFTAFTRCILAIGFIPPSIPKIL
                     HRPFTSLPDENPVGHYFNALYGTGFYYDFIGWMQLLAAVLLLIPKTAHLGAFIQFPIM
                     INIAVLTTSVGFKGTWVIAILMALAGFYLLVWEYDRIKAFLFYTRSAPVSYLKKEGIL
                     LPVLFALGTALLLMVAISIGLANLDKVALLKLFPLVLGGAIFGGMVYWHHRGMQ"
     gene            complement(3944..4708)
                     /locus_tag="PKM27_17665"
     CDS             complement(3944..4708)
                     /locus_tag="PKM27_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNR09153.1"
                     /translation="MRIITYNVNGIRAAMNKGFADWAKSSKADIICVQEIKAKEDQID
                     AGALKKAGYDSYFFPAEKPGYSGVGILTRIKPDNIVTGSGMKAYDEEGRAIRMDLGEY
                     TLVNWYFPSGTSGEVRQDVKMKFLDEVLPWMTRLKEERKNLIVVGDYNIAHTELDIHN
                     PKGNKNTSGFLPEERAWLDKWFASGIHDSFRYLNPEKVEYSWWSVRFNARAQNKGWRI
                     DYQSVTDPVLPLLKEARHLNEVVHSDHCPVQLDLKL"
     gene            complement(4788..5732)
                     /gene="rsgA"
                     /locus_tag="PKM27_17670"
     CDS             complement(4788..5732)
                     /gene="rsgA"
                     /locus_tag="PKM27_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNR09154.1"
                     /translation="MPIQGTVVKSTGNWYQVSAGNKIYPSRIIGKFRLGDKLLTNPVA
                     VGDKVLIELESDGHGIIKTIFSRSNYITRQSPRQRHQLHLLAANVDQAIVVVTLVQPN
                     LKQGFIDRFILMTEPQNIPCSVVVNKCDLFGPEEEEQYQALKQLYESIGYPVMNTSAT
                     MGTGIDDLKKHLRDKITFIGGQSGVGKSTLINAIQPGLDLRTTEISDYTGKGTHTTTF
                     AEMHSLDFGGKIIDTPGIKTLSFNNLEPMDVAHNFREFFTVAQDCRYGDCLHRDEPGC
                     AVKKALDEGRVHPLRYANYCAILEEIEDQNYWERHKEM"
     gene            complement(5736..6509)
                     /locus_tag="PKM27_17675"
     CDS             complement(5736..6509)
                     /locus_tag="PKM27_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNR09155.1"
                     /translation="MIKRRKPLIYPSLVAARQNGRKQMAVLIDPDKLRLGNLDRLLKT
                     SIDSGVHYFFIGGSLIVNNMLDACLEIISDHCEIPLVLFPGNSFQLSDKADAILFLSL
                     ISGRNPELLIGNHVISAPFLKASPLEILSTGYMLVDGGVETTVAYISNTKPIPAHKDD
                     IAVCTALAGEMLGLKLIYLDAGSGAIRPVSESMIEQVSASVDIPVIVGGGIRSPEKAL
                     AQVRAGADLIVVGNALENDPSLAGEIASAIHGYSLQQIT"
     gene            complement(6566..6790)
                     /locus_tag="PKM27_17680"
     CDS             complement(6566..6790)
                     /locus_tag="PKM27_17680"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNR09156.1"
                     /translation="MKWSKIWSTPSELEALGARDLLVAHGIETQILNQKDSMYIFIGE
                     LRLYVPEYKQEEARKVLTINGYLNDPAFLN"
     gene            6856..7503
                     /locus_tag="PKM27_17685"
     CDS             6856..7503
                     /locus_tag="PKM27_17685"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNR09157.1"
                     /translation="MNFNKILTFLVPKNTKFFTQFKQASANLIEAADLLNKIFSKQDF
                     NDRMEFSAEMHKLETKGDDITHSIFSDLSSNFITPFDREDIHEMASTLDTVLDTMDGT
                     AKRVKMYKLDQVSDQMVQLAKLIKAAVLEIDAALHALENVTDPGRVREAIGRIDEIEK
                     HADNIHNEAIADLFQNEKDAIQLIKKKEILQNLEKAVDFCLDTANVIESILIKNS"
     gene            7513..8502
                     /locus_tag="PKM27_17690"
     CDS             7513..8502
                     /locus_tag="PKM27_17690"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNR09158.1"
                     /translation="MTTLLILIIILALLFDFVNGFHDAANSIATVVSTRVLTPLQAVL
                     MAGSANFIAYFIFELHVADTVAKTVHAEHINLMVILAGLIAATVWNLFTWWMGLPSSS
                     SHTLIGGFAGAGIANAGTFNAISLDKIRVILLFIVLAPLIGMVISYFFSLGTMYLCRK
                     LSPHEVDGAFRKLQVGSAAFFSLMHGGNDAQKVVGIIAAALIAQNQITDLKHIPNWVP
                     FSCYVAIGLGTMMGGWRIVKTMGHKVTKLTPFEGFAAETGGAITLLVTGKLGIPVSTT
                     HTITGSIIGTGMLRRVSAVRWGVTISLMWAWIFTIPISAMMGALFFYIGSHLG"
     gene            8580..9398
                     /locus_tag="PKM27_17695"
     CDS             8580..9398
                     /locus_tag="PKM27_17695"
                     /inference="COORDINATES: protein motif:HMM:NF015441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNR09159.1"
                     /translation="MDLRVSQLFIYPIKSLGGIALQDSEVTTRGLRHDRRWMLVDGSG
                     RFLSQREEARMALLEVSLQESSLQVYSKGNPEHRIEIPFLNKREVEFLPRMSVTIWQD
                     QCSPVVYPDAIGAWFSDFLDQTCHLVYMDESVHRPVDVKYAHDQEINSFSDAYPLMMI
                     GQGTLDDLNGRLTEPVPVNRFRPNLVFTGGDPFLEDQMHTFGIRGKVYKAVKPCARCI
                     IPTIDQATGLKGEEPTHTLSTYRLKDRKILFGQNVLVRSEGLIAVGDRLTIDVN"
BASE COUNT         2444 a         2433 c         2429 g         2182 t
ORIGIN      
        1 gtcctcatag agttttttct gggcccatcg ttcgttgcgc gagcaggctt tgataaactc
       61 ttcttcggat tgtaaaggaa tgataatatc catgaccctt ggatttaatc ctccaaatta
      121 aactaaaacg gcgagattca agcccttgct gctttgccgg tcgtcgattt taacgctttg
      181 gcaaaaaaag gggattcctt gcccgtcaac gggctcaggc ccatgccttg ttttcagatt
      241 attactaatt ttaatatgtt tgctccggaa aactacaagc catgtcaaag aaaaaagcgc
      301 cgcccatcat cccggtaccc aaaggggcag aaggcatcaa aatcacctcc ctgaccagca
      361 tgtacgagtc ctatttcctg gattatgcgt cttatgtaat cctggaaaga gccgtgcccg
      421 cgatccagga cggtctcaaa cccgtgcaaa ggaggatcct gcacgctttg tacgaaatcg
      481 acgatggcag gtacaataag gtggccaacg tgatcggcca taccatgcag tatcacccgc
      541 acggggatgc cgccatccgg gatgccctgg tcaaccttgg ccagatggag ctcctgatcg
      601 atacccaggg caattggggg gatgcacgca ccggggccag cgccgcggct ccgcgttata
      661 ttgaagcgcg gctgaccaaa tttgcgctgg aggttttatt taaccccaaa accaccggat
      721 ggttgttgtc ctacgacggc cgccgtaaag aacccgcctt cctcccgtcg aaattcccaa
      781 tcctgctggc ccagggcgtg gagggaatcg cggtgggcct ttccaccaaa atccttccgc
      841 acaattttat agaaatcatc gacgcggccg tgaaatacct cgaaggaaaa aaattcaaat
      901 tgtatccgga ttttcagact gcgggcagca tcgatgtttc agaatatgag gacgggcagc
      961 ggggaggccg ggtgaaaatc cgcgcgaaga tcgaagcggt ggataaaaaa aacctcctga
     1021 tcacggaaat tccctttggc acgaccacct cctcgctgat cgaaagcatc ctgaaagcga
     1081 atgaaaaagg gaagatcaaa atcaaaaaaa tcacggacaa taccgccgct gagattgaaa
     1141 tcgccatcga actgcaaccg ggggtttccc cggatgtgac catcgacgcc ttgtatgcct
     1201 tcaccgactg ccagctttcc gtatcgccca atgcctgcgt catcgtggac caaaagccga
     1261 ggtttgtatg cgtcgccgac attctccggc acaacgtcga ctccaccaag aacctgctga
     1321 gacaggaact ggaaatcaaa agggatgaac tcaccgaggt ctggcatttt gcaaacctgg
     1381 aaaagatctt catcgaaaac cggatctatc acgaaatcga agaatgtgag agctgggagg
     1441 aagtggtgga aaccattcac ctggaattga aaaaatacgt ggccacccct tctgaaaacc
     1501 ccggccccag ggacaagcgc ttaaaactgt ggagagagat caccgatgaa gatgtagccc
     1561 ggctcacgga aatacgcatc aagcggattt ccaaatacaa taccttcaag gccgatgaac
     1621 tgatcctgga gaccgaaaaa tcgctggaac aggtccatta cgacctgaga caccttgtcg
     1681 aatataccat tgcctggttt gaacacctcc gaaaaaaata cggggaaggc aaggaaagga
     1741 ggaccaccat cacccagttt gaaaacatcc aggcccatac cgtagtggcc aacaacacca
     1801 aattgtacgc ggatgacaaa gaaggattca tcggatgggg gctgaagaaa gatcaatccc
     1861 ggctggtcgg agaatgcagc gacatcgacg atatcgtagc ctttcgcaaa gacggcaaga
     1921 tgatcgtctc ccgcatcggg gaaaaggtct tcatgggcaa ggacctgaaa tacgtcaacg
     1981 tctggaaaaa aaatgaagac cgcaccgttt ataatgtgat ctatctggac ggggaaagcg
     2041 gcaaggcttt tgccaaaagg ttccagatcg gcggggtcac ccgggacaag gaatacccga
     2101 tagcgaccga tcacaaggaa agccggctct tatacctcag cgccaacccc aacggtgaat
     2161 ctgaaatcgt aggcatccag ctctcccagg gctgcaccgc acggatcaaa cagtttgact
     2221 ttgatttcgc cggcctcgag atccagggac gcgggtccaa ggggattacc gtcaccaagt
     2281 acccggtgaa acgggtggac ctgaaagaaa aagggaagag ctcactcggc gcccaggcca
     2341 tctggctgga tgaagtcacc ggccgcctga acacggacga aagaggtaaa gccatcggag
     2401 aatttgatac cggagacctg atcctcagcc tgtggaatga cggcagctat cagctcaacc
     2461 ccgtggaaat gagcctgaga tataatgcgg acgaactgat ctggatcgga aaatttgaac
     2521 cggaaatccc cattggggcg gtctattatg atggggacaa aggatggacg atggtcaaac
     2581 ggtttttgat cgaaaccact accaccggat cccgctttaa attcatcacc gagcataagg
     2641 attcccggct gctgtacgcc caggccggag aagacccggt gatcgaatac agctggaaag
     2701 cgaagaatca aaaacattcc cgggagatcc gcctttccga attcatcgat gtcaagggct
     2761 ggaaagccct gggcaaccgg ctgagcgaat ataaactgac cggtatccgg ccaaaggaaa
     2821 acacggcagg cggttccgcg cccaccggca aaaacgccgt tgaatttgag attgtcaacg
     2881 ggaacgggca gaagaaactg ttctgactta agtgaaaggg tgactcggag tcaccccttc
     2941 acttaagtca ccccttcact tgccttcatt tgggaggagc ttggctccga cctttcagtc
     3001 cccctgttgg cttgaagttt gctcagacca tccgttccgg atccatctgt cctgaaaact
     3061 aaaataacag aatttttata gtgatcgcca accctcactg catacccctg tgatgccaat
     3121 acaccatccc cccaaaaatc gctcccccaa gtacaagtgg aaacagtttg aggagggcga
     3181 ccttatccag gttggccagg cctatggaga tcgccaccat gagcaggagg gccgtaccca
     3241 gggcgaatag aacgggtaga agtatcccct cctttttcag gtaggagacc ggggcggaac
     3301 gggtataaaa caaaaacgct ttgatccggt cgtattccca aaccaatagg taaaacccgg
     3361 ccaaggccat caggatggcg atcacccagg tccctttaaa acccactgaa gtggtcagca
     3421 cggcaatgtt gatcatgatc ggaaactgga taaaagcccc caggtgcgcg gtcttcggga
     3481 tcagcaataa gacggccgca agcaattgca tccatccgat gaagtcataa taaaaacccg
     3541 ttccgtacaa agcattaaaa taatggccaa ccggattttc gtccggcagg gaagtaaaag
     3601 gacggtggag tattttggga atgctgggag ggatgaatcc aatagccaga atgcaacggg
     3661 tgaaagccgt aaaaagctga gccggcggcc aggatatcca tgcctggtgc aactggtcca
     3721 aactgcggaa atgcgacatt tcgaattgat tttgctgtaa atgtatatcg ctccatgccc
     3781 taagaagatt ttaatcgcca taagctggaa aggcatggac agacttcaac agggaggagt
     3841 ataaaggccc ggtacgcgta acgggtgttt gaaccggctg cggacgataa attgggtact
     3901 tgaaccaacc gctgcgtttg gattgcagga ttaaatccgc cggctacaat ttgagatcga
     3961 gctgaacggg acaatggtct gaatgcacca cttcattgag atgcctggcc tccttcaaca
     4021 acgggaggac cggatcggtc accgattggt aatcgatccg ccagcctttg ttctgggccc
     4081 gggcattaaa gcgcaccgac caccaactgt attccacctt ctcaggattg aggtagcgga
     4141 aactgtcatg gatgcctgaa gcaaaccatt tgtccagcca tgccctttcc tccggcagaa
     4201 atccactggt atttttattt cccttgggat tgtggatgtc cagttccgta tgggcaatgt
     4261 tgtagtcccc caccacaatc aggtttttcc tctcctcctt caaccgggtc atccagggca
     4321 gcacctcatc gaggaatttc attttgacat cctgcctcac ttccccgctg gtaccgctgg
     4381 gaaaatacca attgaccagg gtatactcgc ccaggtccat ccggatcgcg cggccttcct
     4441 catcataggc tttcatgccg ctgccggtga cgatattgtc aggcttgatc cgggtcagga
     4501 tgcccacccc gctgtacccg ggtttttcag ccgggaaaaa ataactgtca tacccggctt
     4561 ttttaagggc accggcatcg atctggtctt ccttggcctt gatttcctgc acgcagatga
     4621 tgtccgcctt actggacttg gcccaatcgg caaaaccctt gttcatggcg gcgcggatgc
     4681 cgtttacgtt gtaagtgatg atgcgcataa gcggcaaagg tagatgatct cccagaatgc
     4741 gctaaggatg aaataccggg aaagaaagat gcggaagcca ccccgcctca catctcttta
     4801 tgcctttccc aatagttctg gtcctctatt tcttcaagga tcgcgcaata attggcatac
     4861 cggaggggat ggacgcggcc ttcgtccagg gcttttttga cggcgcaacc cggttcgtcc
     4921 cggtgaaggc aatcgccata cctgcaatcc tgggcaaccg taaaaaattc cctgaaatta
     4981 tgggcaacgt ccatgggttc aaggttgttg aatgaaaggg ttttgatccc gggcgtgtca
     5041 atgatcttgc cgccgaaatc cagggaatgc atttccgcaa aggtcgtcgt atgggtgcct
     5101 ttgccggtgt aatccgaaat ttctgtggtg cgcagatcga gccccggctg aatggcgttg
     5161 atcagggtgg acttgcccac cccgctctgt ccgccgataa aggtgatttt gtcgcggaga
     5221 tgttttttca aatcgtcaat cccggtcccc atcgtggccg aggtattcat caccgggtaa
     5281 ccaatggatt catacaattg ttttaaagcc tggtattgtt cctcctcctc cggcccaaac
     5341 aggtcgcatt tgttgaccac aacggagcaa ggaatgtttt ggggctcggt catcaaaatg
     5401 aagcggtcga taaaaccttg tttgagattc ggctgtacca gggtgactac gactatggcc
     5461 tggtccacat tggcggccag gaggtgcaac tggtgtcgct gccgcgggga ctgccgggtg
     5521 atataatttg accttgaaaa aatggttttg atgattccat ggccatcgga ttccagttcg
     5581 atcaacacct tatcgcccac cgcgacgggg ttggtcaaca gcttgtcccc caatctgaat
     5641 tttccgatga tccggctcgg gtaaatcttg ttcccggcgg aaacctggta ccaattgccg
     5701 gttgatttga ccaccgttcc ctggataggc atggatcagg tgatttgctg gagggagtaa
     5761 ccatgaatcg cgctggctat ttcaccagcc agggaagggt cattttccag ggcgttaccg
     5821 accacgatga ggtcagcacc agcccggacc tgtgccaggg ctttttcagg gctgcggatg
     5881 ccgcctccaa cgattaccgg aatatccacc gaggcggaga cctgttcgat catggattcg
     5941 ctcaccgggc ggatggcccc gctgccagcg tccagataga tcaatttcag tcccaacatt
     6001 tctccggcca acgcggtgca aaccgcgata tcgtctttat gcgcagggat gggcttggta
     6061 ttgctgatat aggcaacggt ggtttcgacc ccgccatcga ccagcatata tccggtggaa
     6121 aggatttcaa gcggactggc cttgaggaag ggcgcggata tgacatggtt tccgatcagc
     6181 agttcaggat tacggcccga aatcaaagaa agaaaaagaa tggcatcggc tttatcgctg
     6241 agctggaagg agttgccggg gaaaagcacc agcgggattt cgcagtgatc ggaaatgatt
     6301 tccaggcagg catcgagcat gttattgacg atgaggctgc ccccgatgaa gaaataatgg
     6361 accccgctgt caatggaggt tttgagcagg cggtccaggt tacccagcct caatttgtcc
     6421 gggtcgatca ataccgccat ctgcttccgg ccattttgcc ttgcggccac cagcgaaggg
     6481 taaatcaggg gcttccgacg tttaatcatg gactaaaaaa accaaaggta gctgtaaaat
     6541 ctggattcaa gtcaaccgcg gactttcagt ttaaaaaagc cggatcattt aaatatccat
     6601 tgattgtcaa tactttgcgg gcttcctcct gtttgtattc cggtacatac aacctcaatt
     6661 ccccaatgaa aatatacatg gaatcctttt ggttcagaat ctgggtttca ataccatgtg
     6721 cgacgagcag gtctcttgca cccaatgctt ccagctcgga gggggtactc caaatttttg
     6781 accatttcat cccgaaaact taatattgag ttaacattga agatataatt ttgaacattt
     6841 ttaatccgac ccttcatgaa ttttaacaaa attctgacct ttctggtacc taaaaacacc
     6901 aaatttttca cccagttcaa acaagccagc gccaacctca ttgaagctgc cgacctcctg
     6961 aataaaatat tctcaaagca ggattttaac gaccggatgg agttttcagc agaaatgcac
     7021 aaactggaaa ccaaggggga tgatatcacc cattctattt tcagtgacct gagctccaat
     7081 ttcatcaccc cttttgaccg ggaggacatc catgaaatgg catccaccct ggataccgtg
     7141 ctcgacacca tggatggcac tgcaaaaaga gtgaagatgt ataaattgga ccaggtcagt
     7201 gaccagatgg tccagctggc caaactcatt aaagcggccg tcctggaaat cgacgctgcg
     7261 ctccatgccc ttgagaatgt aaccgatccg gggcgggtac gcgaagccat cggccggatc
     7321 gatgagattg aaaaacacgc ggacaatatc cataatgaag ccattgccga cttgttccag
     7381 aacgaaaagg acgcgatcca gctgatcaag aaaaaagaga tcctgcagaa cctggaaaaa
     7441 gccgttgatt tttgtctgga taccgccaac gtcattgaaa gcatattgat aaaaaattct
     7501 taaggcagga cgatgaccac cttgcttatc ctgattatca tccttgcctt attatttgat
     7561 tttgtcaatg gttttcatga tgcggccaat tccattgcca ccgtggtttc cacaagggtt
     7621 cttacccctt tacaggccgt tctcatggcc ggctcagcca actttatcgc ttattttatt
     7681 tttgaacttc atgtcgccga cacggtggcc aaaaccgtgc atgctgaaca catcaacctg
     7741 atggtcatcc tggccgggct gatcgcagcc accgtttgga atctttttac ctggtggatg
     7801 ggattaccca gcagttcttc ccataccttg atcggcggct tcgccggtgc cgggatcgcg
     7861 aacgcaggta ctttcaacgc catttccctg gataaaatcc gggtcatctt actgtttatc
     7921 gtcctggccc cactgatcgg catggtcatc tcctattttt tttccctggg cacgatgtat
     7981 ctctgcagga agctgtcacc ccatgaggta gatggcgctt tccggaaatt gcaggtcggt
     8041 tccgccgctt ttttcagtct gatgcatggc gggaacgacg cgcagaaagt ggtcgggatc
     8101 attgccgccg cccttatagc tcaaaaccaa atcaccgatc tgaagcatat tcccaactgg
     8161 gtccccttca gttgttatgt ggccattggc cttggcacca tgatgggcgg ctggcgcatt
     8221 gtaaaaacaa tggggcacaa agtcaccaaa ctcacacctt ttgagggttt tgccgccgaa
     8281 accggcggag cgatcactct tttggttacc ggcaaactgg gcatcccggt cagcaccacc
     8341 cacaccatca caggaagcat cataggaacg ggcatgctga ggagggtcag tgcggtaaga
     8401 tggggggtca ccatcagcct gatgtgggcc tggatcttca ccatcccgat ttcagccatg
     8461 atgggggcct tattttttta cataggaagt catttgggat aggagtgagg agtgaggagt
     8521 gagggttgag gagtgaggag tgagggttga ggagtgaggg ttgaaatcgc tttaagatta
     8581 tggatttacg ggtttcacag ttgtttattt atcctatcaa gtcattgggg ggtatcgcgc
     8641 ttcaagactc ggaagtcacc acccggggac tccggcatga ccgccgttgg atgttggttg
     8701 acgggtccgg aagattttta tcccaaagag aggaggcccg tatggctttg ttggaggtaa
     8761 gtcttcaaga atcctccctt caggtctact caaagggaaa ccccgaacac aggattgaga
     8821 tcccctttct gaataaaagg gaggtggaat ttctgccacg gatgtccgtg acgatatggc
     8881 aggatcagtg cagcccggtg gtttatcccg atgcgatagg cgcatggttt tcggatttcc
     8941 tggaccaaac ctgccacctg gtatatatgg atgaatcggt acaccggcct gtggatgtca
     9001 aatatgccca tgaccaggaa atcaactcat tcagtgatgc ttacccccta atgatgatcg
     9061 gccagggcac cctggatgat ctcaatgggc ggctgacgga accggttccg gtaaatcgat
     9121 tcaggccgaa cctggtattc accggaggcg accctttcct tgaggatcaa atgcacacct
     9181 ttggtatccg gggcaaggtg tacaaagcag tcaaaccctg cgcccgttgc atcattccga
     9241 ccattgacca ggccaccggg ctaaaaggtg aagaaccaac ccacaccctg tccacctacc
     9301 gtttgaaaga cagaaaaatt ctgttcggac agaatgtgct cgtcaggagt gaagggttga
     9361 tcgcagtggg agatcgactg actatagacg tgaattgatc cctggacttc gccttgccct
     9421 ccgaatccgc ctcacaggta attgccttta gacccaaact tttttcgctg catgtaatga
     9481 tatgcttc
//
LOCUS       DAOQXT010000146         9407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_7796_length_9407_cov_7.294803, whole genome shotgun sequence.
ACCESSION   DAOQXT010000146 DAOQXT010000000
VERSION     DAOQXT010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7796_length_9407_cov_7.294803"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..1937
                     /locus_tag="PKM27_17700"
     CDS             420..1937
                     /locus_tag="PKM27_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNR09160.1"
                     /translation="MSLFIRKPLSQLLAQAEDGEKSLKRTLTATSLVLLGIGAIIGAG
                     LFVRTAAAAGGHAGNAVTISFIIAAIGCALAGLCYAEFAAMIPIAGSAYTYSYATMGE
                     LVAWIIGWALVLEYALGAATVSIAWSQYLNKIFGGAIPFEWCHSPFEYLVNRNGIEVV
                     TTSLQAGEAAITNGIINLPALLILLLISLVLIKGTQESAIVNAVIVFIKVAIVLIFIA
                     IGWGFINPDNHTPYFIPQGDPGSAYEGFFNHGWGGVLRGAGIVFFAFIGFDAVSTAAQ
                     EAKNPSKDMPIGILGSLVICTVLYILFSHVLTGVAPYTDFVNAGKEASVAYAIQTYMT
                     GYSWLATAITIAILAGFSSVILVMLLGQSRVFYSMSKDGLLPGVFSELHSKFRTPYKS
                     NMILFVFVSIFGGFFPESWAGDLTSIGTLFAFVLVCIGVILMRRSDPDIPRTFKTPWV
                     PVVPILGAIVCAIMILGLPAGTQLTALGWMVIGLVIYFLYSARHSKLRNPAAAKK"
     gene            2068..3141
                     /gene="prfA"
                     /locus_tag="PKM27_17705"
     CDS             2068..3141
                     /gene="prfA"
                     /locus_tag="PKM27_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNR09161.1"
                     /translation="MQDKLEAIERRFYDLEEQMADPGVISDSQRFSKIHKEYKDLEPI
                     VRAFRRQTQCLAELKGAREMLQEGDEELRAMAKEEIAGLEEEMEALGDEIKKLLIPKD
                     PEDSKDIILEIRSGTGGDEASIFAGDLLRMYNRYFEKQGWKTELLYVNEGAAGGYNKV
                     VLEVTGEDVYGKMKFESGTHRVQRVPKTEAQGRVHTSAATVAVMPKLELEDVNINRAD
                     LRIDTFRSSGAGGQHVNKTESGVRFTHLPTGVVAESMDSRSQHKNREIALGRLIEKIQ
                     EARVQQHQDSIAAQRKSMVGSGDRSEKIRTYNFPQNRLTDHRINLTLYNLDSIMDGNI
                     DDVIEKLRLAENAERMNGLQQGV"
     gene            3286..3951
                     /locus_tag="PKM27_17710"
     CDS             3286..3951
                     /locus_tag="PKM27_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNR09162.1"
                     /translation="MASQTVENYIKAIFQLTEREGGSVSTSAISQQLATSAASVTDML
                     KKLAEQKLIAYVKYRGVHLTPGGKSMAVEMIRKHRLWETFLHDKLAIGWDAVHEIAEQ
                     LEHVQSATLIDQLDAFLGYPKFDPHGDPIPDANGKFTLRNQVLLNTLQEGDQAQVVGV
                     REHDADFLKYLDSRALHINASLLIKEKQSYDGSVRVETAGGAETLLSSKAAQNIYVKK
                     NLN"
     gene            3961..4959
                     /locus_tag="PKM27_17715"
     CDS             3961..4959
                     /locus_tag="PKM27_17715"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNR09163.1"
                     /translation="MIKSIICLLAGIPLFGQDEKPLILGSASIFMDMAKQVGGEHFRY
                     DAIVPVGGDPHIYEPIPRDVQKCLSASLILRNGLTFEGWLGELIENSGTKARTILITE
                     GIEAIESLKYKNSSDPHAWMTASNGVIYAENIRKGMLTLSPGDSASINRNFEAYKQKL
                     EELDRYIYQQVSSIPAEKRILITSHDAFQYYGRRYGIRLEAVLGTTTDADVQTGDILR
                     LNKVIRENQLPAVFVESTVNGKLLEQVARDNRIGIGGKLFSDSIGDEQSPAPTYYDML
                     KYNTDVIVAALTKPLDSQAGNTADSSKKTRRILLWSVFLLALLGGGIYWFNKRSEK"
     gene            4956..5696
                     /locus_tag="PKM27_17720"
     CDS             4956..5696
                     /locus_tag="PKM27_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNR09164.1"
                     /translation="MSTPNIQINGVSVTYDHKRVLSNIFLEIEAGKIYGLVGPNGAGK
                     STLFKAILGLVEIQSGLIRFGEQSVEEWRQKIAYVPQKDEVDWSFPATVLDIVLMGRY
                     PYKKWYARLDKKDHEMAMQVLKKVGMADFSRRQIGELSGGQQQRVFMARALCQGAAYY
                     FLDEPFAGVDITTEEKIIEILKELAMEGNTLLVVHHDLATVSRYFDRVILLNQRLIAA
                     GDIATTFTDENVRKTYGGQLSILHKLKN"
     gene            6083..7183
                     /locus_tag="PKM27_17725"
     CDS             6083..7183
                     /locus_tag="PKM27_17725"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNR09165.1"
                     /translation="METTIKPVFKKGSGTDIYARLRREATKLVEQLEPLRRNTILFKA
                     FLFPLAYCATWLLALVFAPSPGVYYFCFFLLGIQLVSIFLNIIHDAVHGTLFKGKKAN
                     RIMVYLFDLMGANSFIWRQRHIRFHHNYPNVLGWDTDVDQSRLFRLFPSAPHYPMNRY
                     QHLYLPLVYPLFLFNWLLLRDFRDFFNKRKMVHKLIRIPAAEYVKLMFFKVFFLAYTL
                     FIPKFGAGIPWRQAIAGFVMMVLTASVFSLITLLPPHANTENKFPIPDEKNEMPEDWF
                     MHMLETTNDVKESNGFTRFFLGNFNFHVVHHLFPQINHVYYPELTHLLEKYASEYHLP
                     YRKYPLFRTLANHYRLVRQNRVSVNALFGETM"
     gene            complement(7195..7545)
                     /locus_tag="PKM27_17730"
     CDS             complement(7195..7545)
                     /locus_tag="PKM27_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09166.1"
                     /translation="MNIKYFPALIFLSFVFACSYNSSELDCTAVSGARFSSSGGQMQA
                     LIQNKCSGNDCHSTGGQGSVHWTLGPYNDIKQHFDHMYEAVKSGSMPKAGSAPLTAEE
                     LDRFECWHQSGYPE"
     gene            complement(7687..8133)
                     /gene="rplI"
                     /locus_tag="PKM27_17735"
     CDS             complement(7687..8133)
                     /gene="rplI"
                     /locus_tag="PKM27_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNR09167.1"
                     /translation="MEIIMLQDVEKVGDKHTVVKVKDGYGRNFLIPKGLAIIANKANR
                     GRLEEMLRHEAAKEAKLVDTYREMAKKLVGQTLKIVVKAASTGKIFGSVSTVQIAQTL
                     KDQLGLEVERKKISLPEEVKTLGTYTANVNFHKDVPATVAFEVVEG"
     gene            complement(8159..8425)
                     /gene="rpsR"
                     /locus_tag="PKM27_17740"
     CDS             complement(8159..8425)
                     /gene="rpsR"
                     /locus_tag="PKM27_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNR09168.1"
                     /translation="MASQEEIKFLSNPTLGQQRKKYCRFRKFGIKHIDYKDPDFLIQF
                     VNEQGKLLPRRITGNSLKYQRRVSSAVKRARHLAMLPFSTDLLK"
     gene            complement(8447..8824)
                     /gene="rpsF"
                     /locus_tag="PKM27_17745"
     CDS             complement(8447..8824)
                     /gene="rpsF"
                     /locus_tag="PKM27_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNR09169.1"
                     /translation="MRQFEVTFIVDPVLSDDEIKSAAKNYSDMIVHEGGKIVHLDEMG
                     LRQLAYPINKRNTGVYFSLEFQSESGAFIPKMELSMKRDERILRFLTVRLDKYGVKYN
                     EDKRNGLIGKRKDWKKKKAEDEV"
BASE COUNT         2412 a         2220 c         2392 g         2383 t
ORIGIN      
        1 tggtaggatt ggtaggattg gtaggattgg taggattggt aggattggta ggattggtag
       61 gatttggcat tataataaaa aaaggaagat ttccggtccc cggccttcat tcccatctat
      121 ccaaaaaatc tgatgtttcc ataaacccat taaatcttag caatccatta aatcttataa
      181 atcctataaa tcctataaat cctataaatc ctattaatcc tagtcttttt tagcatagca
      241 tttaaggttt tcgtttttga atgcctgtcg tttgaagcgg cgggcatttt ttttagtagt
      301 cagtagtcag tagtcagcaa tcagcagtca gcagtcagca gtcagtaact actgatagct
      361 gaatactgaa agctgattac taaaattttc ctattttgac ggccctaaag aaaccataaa
      421 tgagtctgtt tatacgaaaa cctctcagcc agctcctggc ccaggcagag gatggagaga
      481 aatcacttaa aaggacgctg acggccacct ccctggtgct cctggggatc ggggccatca
      541 tcggggctgg gctgttcgtt cggacggcag ccgctgcggg agggcatgca gggaacgcgg
      601 tgaccatttc atttatcata gccgctattg gttgcgcact cgccggcctg tgttacgcgg
      661 agtttgccgc catgattccg attgcaggca gcgcctatac ctactcctat gccacgatgg
      721 gtgaactggt cgcctggatc attggttggg ccctggtatt ggaatatgcc ctcggagctg
      781 cgacggtgtc cattgcctgg agccagtacc tcaataaaat tttcggggga gcgatcccct
      841 ttgaatggtg tcattccccc ttcgaatatc tggtgaaccg aaatgggata gaggtggtga
      901 ccaccagtct tcaggccggg gaagcggcca tcaccaacgg aatcatcaat ttaccggcat
      961 tgctgatcct tttgcttatc agcctggtcc tgatcaaagg cacccaggaa tccgccatcg
     1021 tcaatgcggt cattgtgttt atcaaagtgg ccatcgtgct gatatttatt gccattggct
     1081 ggggatttat aaatccggac aaccataccc cctattttat tccgcagggg gaccctggca
     1141 gcgcttatga aggttttttc aaccatggct ggggaggggt gcttcggggt gcaggaatcg
     1201 tgttttttgc atttataggt tttgatgccg tatccaccgc agcacaggag gcgaaaaatc
     1261 ccagcaagga catgcccatt ggcatccttg gctcactggt gatttgtaca gtgttgtaca
     1321 tcctgttttc tcacgtcctg accggggtag ccccatacac tgattttgtg aacgcgggta
     1381 aggaagcctc ggtggcttat gccatccaga cctatatgac gggatacagc tggctcgcca
     1441 ccgccattac gatagccata ttggccggtt tttcatcagt aatcctcgtc atgctcctgg
     1501 gccaatccag ggtgttttat tccatgtcga aggatggctt gttgcccggg gtcttttcag
     1561 aactgcactc caaattcagg acgccgtata aatccaatat gatccttttc gtatttgtgt
     1621 ccatttttgg tggatttttc ccggagagtt gggcagggga tttgacctcg atcggcacct
     1681 tgttcgcttt tgtcctggtc tgcattgggg tgatcctgat gcgccgctct gatccggaca
     1741 ttcccaggac ctttaaaacc ccctgggtac cggtagtgcc gatactcggg gccatcgttt
     1801 gtgccatcat gatcctgggt ctgcccgcgg gcacccaact gaccgcactg ggttggatgg
     1861 tcatcggcct ggtcatctat ttcttgtata gcgccaggca cagtaaactc aggaacccgg
     1921 cagcggctaa gaaatagggc caaccatacc ctgctccttt taaagacccg ggacccaggg
     1981 tgattgccag agtgtgctat gcaacgcgta actttgtatt ttttaaatac atggcctcag
     2041 gctggttttt tttagcaagc gtttcaaatg caggataaac tggaggcgat cgaacgccgt
     2101 ttttatgacc tggaggaaca aatggctgat ccgggagtga tcagcgattc ccagcgattt
     2161 tcgaaaatac ataaggaata caaggatctg gaacccattg tgcgggcatt ccggagacag
     2221 acgcaatgcc tggctgaatt aaaaggcgcc cgggaaatgc tccaggaagg agacgaagaa
     2281 ctgcgtgcca tggccaagga agaaatcgcc ggcctggagg aagaaatgga agccctcggg
     2341 gatgaaatca aaaaattgct gatcccaaaa gatccggaag acagcaagga cattatactt
     2401 gaaatccgtt ccgggaccgg cggcgacgaa gccagcatct tcgcgggtga tttgctgcgg
     2461 atgtacaacc ggtatttcga aaagcagggc tggaaaacgg aactgcttta tgtgaacgaa
     2521 ggagcagcgg gcggctataa taaagtggtg ctggaagtca ccggagagga cgtctatggg
     2581 aaaatgaaat ttgagtccgg aacccaccgc gttcaaagag tccccaagac cgaagcccag
     2641 ggcagggtgc acacctctgc ggctacggtc gcggtcatgc ccaaactgga attggaggat
     2701 gtcaacatca accgcgccga cctccggatc gatactttca ggtccagcgg tgcgggaggg
     2761 cagcacgtaa ataagacgga aagcggggtt cggtttaccc accttccgac cggggtggtg
     2821 gctgaaagca tggacagcag gtcccagcat aaaaacaggg agatcgccct cggccgcctt
     2881 atcgaaaaaa tccaggaggc ccgggtacag caacaccagg attccattgc agcacagcga
     2941 aaatcaatgg tgggcagcgg cgaccgctct gaaaaaatca ggacctataa ttttcctcaa
     3001 aaccgcctca ccgaccaccg catcaacctc accttataca acctggattc catcatggat
     3061 ggcaatatcg acgatgtcat cgaaaaactg cgcctggcgg aaaatgcgga gaggatgaat
     3121 ggacttcagc aaggggttta gttttcgtcc cggatttctt atatgagctt tgggtgatct
     3181 ccgtgacttc tgtgaaagtc aaaaatcctg cataaaacca ggcaaccaat aaaataaatt
     3241 ttaaactatt aaaaaataaa tttagattta tctaaattta tttaaatggc aagtcaaact
     3301 gttgagaatt atattaaagc catcttccag ttgacggagc gggaaggggg ttccgtgagc
     3361 acatcggcca tctcccagca gctcgccacc agcgcggctt ccgtcaccga tatgttgaaa
     3421 aaactggccg aacaaaaact aatagcatac gtcaaatacc gcggggtgca cctcactccc
     3481 ggaggcaagt ccatggccgt ggaaatgatc cgcaagcacc gtctttggga gaccttttta
     3541 cacgacaaac tggccatcgg ctgggacgcc gtccatgaaa tcgccgagca actggagcat
     3601 gtgcagtcgg ccaccctgat tgaccagctg gacgccttcc tgggttatcc caagtttgac
     3661 ccgcacgggg atcccatccc ggatgccaat ggcaagttta ccctacgcaa ccaggtgtta
     3721 ttgaataccc tgcaggaagg ggatcaggcc caggtggtag gggtgaggga acatgatgcg
     3781 gattttttga aatacctgga ttcccgggcc ttgcacatca atgcaagcct gttgatcaaa
     3841 gaaaaacaga gttatgacgg atcggtgaga gtggagaccg ccggcggagc ggaaaccctg
     3901 cttagttcca aagccgccca gaatatttat gtgaaaaaaa acctgaactg aaccatgaag
     3961 atgataaaat caataatatg cctcctggcc ggaatccctc tctttgggca ggacgaaaag
     4021 ccactcatat tggggtccgc ttccatattt atggatatgg caaaacaagt tggaggcgaa
     4081 cacttcagat acgatgccat tgttccggta ggcggcgatc cgcatattta cgaacccatt
     4141 cccagggacg ttcagaaatg cctgagcgct tccctgatcc tgagaaatgg cctgaccttc
     4201 gaaggttggc tgggagagtt gattgagaat tcagggacca aagccaggac catcctgatc
     4261 accgaaggca tcgaagccat cgaatccctc aagtacaaaa actcctcgga tccccacgcc
     4321 tggatgacgg cgagcaacgg ggtgatttat gccgaaaata tccggaaggg catgctgacc
     4381 ctgtcgcccg gggacagtgc ctccatcaac cggaattttg aagcctataa acaaaaactc
     4441 gaagaactgg accgctacat ctaccagcag gtctccagca taccggccga aaaacggatc
     4501 ctgatcacct cccacgacgc gttccaatat tatggaaggc gttacgggat ccgcctggaa
     4561 gcggtcctcg gcaccactac ggatgccgat gtgcagaccg gcgatatcct tcggctcaac
     4621 aaagtcattc gggaaaacca gctaccggct gtcttcgtcg aaagcacggt aaacggtaaa
     4681 ttgctggaac aggtggcccg ggacaaccgg atcgggatcg gaggcaaact tttttctgat
     4741 tccatcggag atgagcaaag tcccgcccca acttattacg acatgctcaa atacaatacg
     4801 gacgtcatcg ttgccgccct gaccaaacct ctggatagcc aggcggggaa tacggcagac
     4861 agttcaaaaa agacccggcg catcctactt tggtccgtat tcctgctggc tctattgggg
     4921 ggcggtattt actggttcaa taaaaggtct gaaaaatgag tacccctaat atccagatca
     4981 acggcgtctc ggttacttac gatcacaagc gggtcctgag caatatattt cttgaaatcg
     5041 aagccggtaa aatatacgga ctcgtcggac ccaacggggc tgggaagtcc accttgttca
     5101 aagccatcct gggcctggtg gaaatccagt cgggacttat tcgttttggg gagcaatctg
     5161 tggaggaatg gcggcagaaa atagcctatg tcccccagaa ggatgaagtg gactggagtt
     5221 ttccggccac cgtcctggac attgtattga tgggaagata tccttacaaa aaatggtatg
     5281 cccggctgga taaaaaagat catgagatgg ccatgcaggt attgaaaaaa gtgggcatgg
     5341 ccgacttcag ccgcaggcag atcggtgaac tttccggcgg gcagcaacaa cgggtgttta
     5401 tggcccgcgc cctttgccag ggagccgcct actatttcct ggatgagcca tttgccggcg
     5461 tggacatcac gacggaagaa aaaatcattg agattttaaa ggaactggcc atggagggca
     5521 acaccctgct ggtcgtgcat cacgacctgg ccacggtcag ccgttatttc gaccgggtga
     5581 tcctgctgaa tcagcgtttg attgcggcgg gagatatagc gaccaccttt acggatgaaa
     5641 atgtgaggaa gacttatggg gggcagttga gtatactgca taaattgaaa aactgaagtc
     5701 aaaggttaag ggtcaacgga gaacggttaa aggagaaagg agacgggaaa cgggagacgg
     5761 agtgcccatg cctttcagcg taattttttt tatatttatt ttgattttaa tgaaccgttc
     5821 gaagggaatt acctgagaac cacatgcttt tttaaaaccc aaatcatgca atagagtttc
     5881 tcaatgaaag ccggaaagcc aatacagact ggcgctccag gactattttt atcgcaaaca
     5941 tggtcttccc tatggtgagg ataataatgg gaggccgacc ttcatactaa atcaacctgt
     6001 tcggccggga agtgccgtta cttgaaggca ttcggcttgt aatagaaaat cgattgcatg
     6061 gtttggatta aaatattggc ctatggaaac gaccataaaa ccagtgttca aaaaggggag
     6121 tggaacagac atctatgcac gactcagaag agaagccacg aagctggtgg aacagcttga
     6181 gccattaagg cggaacacga tcctcttcaa ggccttttta ttccctttgg cctactgtgc
     6241 gacctggctg cttgccctgg tcttcgcacc atcgcccggt gtgtattatt tctgtttttt
     6301 tttactgggg attcagttgg tctctatttt tctcaatatc attcatgacg ccgttcacgg
     6361 caccctgttt aaagggaaaa aagccaaccg gattatggtg tacctgtttg atctgatggg
     6421 ggccaacagt tttatatgga gacagcggca tatccggttc catcataatt atccaaacgt
     6481 cctgggctgg gataccgatg tggatcaaag ccgcttgttt cgcctctttc cctctgctcc
     6541 gcattacccc atgaaccggt accagcattt atacctgccc ctggtctatc cgctttttct
     6601 gttcaactgg ttgttgttgc gggatttccg cgactttttt aataaaagaa agatggtgca
     6661 taaactgatc cgtatcccgg ccgcggaata tgtgaagttg atgttcttca aagttttttt
     6721 tcttgcctac accctgttca ttcccaaatt cggggcagga attccgtgga ggcaggccat
     6781 tgccggcttt gtgatgatgg tcctgaccgc cagtgttttt tcacttatta ccctgctgcc
     6841 cccgcatgcc aatacggaga acaaattccc cataccggat gaaaaaaatg aaatgccgga
     6901 ggattggttc atgcacatgc tggagaccac caacgatgtg aaggaaagca atgggtttac
     6961 ccggtttttc ctcgggaatt ttaatttcca tgtggttcat catttattcc cccaaatcaa
     7021 ccatgtttat tacccggaac tgacccacct cctggaaaaa tatgcttcgg aatatcattt
     7081 gccttatcgg aaatatcccc tcttccggac cctggccaat cattatcgac tggtccgcca
     7141 gaaccgggtt tccgtaaacg cccttttcgg tgaaacgatg taggaaggat ggaattattc
     7201 cggatatccc gactggtgcc agcattcgaa acggtccagt tcctcggcgg tcaggggagc
     7261 ggagcccgct tttggcatgg atcctgattt tacggcctcg tacatgtggt caaaatgctg
     7321 tttgatatca ttgtagggtc ccagcgtcca gtggaccgag ccttgtcccc ctgtcgaatg
     7381 gcagtcattc ccggaacatt tattttggat cagggcctgc atctggccac ctgaggaaga
     7441 aaaccgggca cccgatacgg ccgtgcagtc cagctccgag gaattgtaag aacaagcgaa
     7501 gacgaaagaa aggaagataa gcgcaggaaa atattttata ttcatgatgc agcgggatat
     7561 ttagtcgtta acgatggaag catccgcttc gttaaccgat ccaaaatttt acacggcaaa
     7621 tatgaataaa aaaaaaccgc agccggtata atcgactgcg gtttgatata agattttgct
     7681 taaagcctaa ccttcaacca cctcaaaagc gaccgtcgcg gggacgtcct tatgaaagtt
     7741 gacgttggca gtataggtgc ccagggtttt gacttcctcg ggaaggctga tttttttcct
     7801 ttccacttcg aggccgagct ggtctttaag ggtctgtgcg atctgcacgg tacttacgct
     7861 gccgaaaatt tttccggttg aagcggcttt gaccacgatt ttcagggtct ggcctaccag
     7921 tttcttagcc atttcacggt aggtgtccac cagtttggct tctttggcgg cttcgtggcg
     7981 cagcatttct tcaaggcggc cccggtttgc tttattggca atgatggcca ggcctttggg
     8041 gattagaaaa ttgcggccgt atccgtcttt tacttttacc acggtatgtt tgtcgcccac
     8101 tttttcaaca tcctggagca tgatgatttc catattctga tcgattgacg gtgaaaaatt
     8161 attttaataa atcggttgag aatggcagca tggccaggtg ccttgcccgc ttaacggcgg
     8221 aagacaccct tctttgatac ttcagggaat ttcccgtgat gcgccggggc agcagtttgc
     8281 cctgctcatt cacaaactgg atgagaaaat ccgggtcctt gtaatcgatg tgcttgatgc
     8341 cgaatttgcg gaaacggcaa tattttttcc tttgctgacc cagggtggga ttggagagga
     8401 atttgatttc ttcttgtgaa gccatttttt ttcttttttc ggtgatttaa acttcgtctt
     8461 ctgctttctt tttcttccag tccttgcgtt tgccgatcag gccgttgcgt ttgtcttcgt
     8521 tgtatttcac gccgtacttg tcgagcctta cggtcaggaa ccgcaatatg cgttcatccc
     8581 ttttcatgga cagctccatt ttggggatga aggctccgga ctcggattgg aattcaagac
     8641 tgaaatagac cccggtgttg cgcttgttga tgggataagc cagctgacgc aggcccatct
     8701 catcaaggtg tacgatcttg ccgccctcat ggacgatcat atcggaatag ttttttgctg
     8761 ccgacttaat ttcatcgtcc gacagcactg gatctacgat gaaagtcact tcgaattgtc
     8821 tcattgcgaa atggttaatt aaattttggg ctgcaaaggt aataaagatt tgggaatttg
     8881 ggaagggagt ataggggaga atctgggaaa tgggttctca cgaagagtga ggttcccaca
     8941 aagacgcaaa gttcatgaag agtgaggttc ccacaaaggc gcaaagttca ctaagagtgg
     9001 ggttcccaca aagacgcaaa gttcgcgaag agtggagttc ccacaaagac gcaaagatca
     9061 cgaagatttt attcttttct tttgataatc aataatttgt gccctttgtg tcttagtggg
     9121 agaaattaag aggccgaagc gaagcttcgt gcccgcaaag agaatggtgt cccacaaaga
     9181 cgcaaagttc acgaagagtg gagttaccac aaagacgcaa agttcacgaa gatcttattc
     9241 ttttattttg ataatcaata atttgtgtct tcgtgtctta gtgggagaaa ttaagaggcc
     9301 gaaacgaagc ttcgtccaaa cagtaccttc ccgcaaagag aatggtgtcc cacaaagtta
     9361 taccgttaaa aaccaaatta ttttcaaaca atttttataa gtttgat
//
LOCUS       DAOQXT010000147         9404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_7799_length_9404_cov_5.260990, whole genome shotgun sequence.
ACCESSION   DAOQXT010000147 DAOQXT010000000
VERSION     DAOQXT010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9404
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7799_length_9404_cov_5.260990"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKM27_17750"
     CDS             <1..465
                     /locus_tag="PKM27_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR09170.1"
                     /translation="YHYLDKEKVRLVAVEAAGEGIHSGRSAATSILGSVGIIHGSKTL
                     LMQTADGQIIEPHSISAGLDYPGIGPLHAHLIRTGRAEVIAITDEEAVKAAFVLTRLE
                     GIIPALESAHALAALDQMQYRPGENIVICLSGRGDKDLATYMKYLDTYESIV"
     gene            449..1225
                     /gene="trpA"
                     /locus_tag="PKM27_17755"
     CDS             449..1225
                     /gene="trpA"
                     /locus_tag="PKM27_17755"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNR09171.1"
                     /translation="MSRLFDLFARKQGGILNIYFTAGFPGLEDTVPIIKALSDAGADL
                     VEIGIPYSDPLADGPTIQASGQAALKNGMTLDLLFRQVAQARAYTSMPFILMGYFNQV
                     LQFGEENFLSRAKESGVDALILPDLPVEEYELHYRSLFGKYGMDIAFLITPQTPEARI
                     RHIDQVSNSFIYMVSSNAITGAKAGIDDYQEAYFRRISAMGLKNPRLIGFGISNKATF
                     DQACRFAQGAIIGSAFINALAGSKDLASTVSQFIGQVRQG"
     gene            1502..2383
                     /locus_tag="PKM27_17760"
     CDS             1502..2383
                     /locus_tag="PKM27_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNR09172.1"
                     /translation="MQLLDGVLVAKKIKADLLQLVKAEQDKGKRVPHLAAVLIGDHPA
                     SASYIKNKIRSCAEAGYESTLIHRDSSITKEGLLEIVDGLNRNEAIDGYIVQLPLPRH
                     IDEQEILLAIDPKKDVDGFHPMNIGRMVIGLPAYVPATPLGILKLIEHYRIPTEGKHV
                     VVIGRSNIVGTPVSLLLSRKAYPGNATVTLCHSKSTDLAEMSRKADILIAAIGTPHFV
                     KADMIKDGAAVIDVGMNQIADPASKKGHRLVGDVDFEGVSHKAAYLTPVPGGVGPMTV
                     CALMINTWTAYTGKIYG"
     gene            2376..3194
                     /gene="prmA"
                     /locus_tag="PKM27_17765"
     CDS             2376..3194
                     /gene="prmA"
                     /locus_tag="PKM27_17765"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNR09173.1"
                     /translation="MDEINQNYYRLVFTSLPDEITLVHLYECGLEAVEEKETHIEGYL
                     PESLYSPALLDKLRSILPIRTSERLEPQNWNAIWESSFQPVWVDGKVHIRASFHEKSP
                     GGMDVVIDPKMAFGTGHHATTSMVIREMLDLDLEGRAVLDFGCGSGLLSIVAEKLGAG
                     RIKALDYDPWSVENTKENMKVNQSRHIEVKQADSLNEETGAYDLILANITRDVLIRNT
                     PDISRLLKPGGHAVYSGFLSGDRPILETEIRKYGMEPGKSAEESGWGVLVMTKT"
     gene            complement(3249..3761)
                     /locus_tag="PKM27_17770"
     CDS             complement(3249..3761)
                     /locus_tag="PKM27_17770"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNR09174.1"
                     /translation="MKTLIFSLFCTLCLSALSTAQEQSLNQFVRANQNRDGIRHFSIP
                     GFLVRLAGGIALREEDQWQREAMRPLVRNLGSISVMFSDGGEGFRDEDVRTLKKNLVA
                     ENYEPLVSLRDNGSRVEVFAWQKKDILRRVVFLIHEKHDESVLVSLRGHFTQDDISKM
                     LRSFEKEEII"
     gene            complement(3764..4300)
                     /locus_tag="PKM27_17775"
     CDS             complement(3764..4300)
                     /locus_tag="PKM27_17775"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNR09175.1"
                     /translation="MKSIITMKNFIFLPLLFSTILATAQSDAVTRFFKAYQDDERFTS
                     VYISPKMFQLVSKIKLDDMDPELRSLLQTIKGLHILSTEDSPQAFYKEAVGKINTQEY
                     EELMKVRDKGEDVLFLTKSNAANPDKIDELLMLVGGPDEFTLVSFVGDIDLSKVGRLG
                     RTLNIEGSEHLEKIQKKN"
     gene            complement(4306..4794)
                     /locus_tag="PKM27_17780"
     CDS             complement(4306..4794)
                     /locus_tag="PKM27_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09176.1"
                     /translation="MNNSKEYIKQLLDKYWAGESTLEDEKNLAAYFHAGQVDEAFKAF
                     QPLFDYFDQERQVTIDLEDQIMDRILHPRSETKIVRLTWKRALAVAASLILLVTAGIG
                     TYRYQHSQKDQLAIRDTFESPEEALEQTKAALLYLSNRMNRVADQTTKSLEKTENLDI
                     LN"
     gene            complement(4781..5290)
                     /locus_tag="PKM27_17785"
     CDS             complement(4781..5290)
                     /locus_tag="PKM27_17785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR09177.1"
                     /translation="MDPQEFRQFVKANQSKIFRYALHLLQKEEDAKDIVQDVLIKIWD
                     RRDELDRIENLEGWMFTVTRNLCIDRIRARKNHLDIGTQVQVQDSQDNPHEKTAKAQV
                     MNLIKKLIGEMPEKQKMVLRLRDIEGLSYDEIAEACSLSLAQVKVNLHRARLNLKEKL
                     IQSGLYEQL"
     gene            5480..7213
                     /locus_tag="PKM27_17790"
     CDS             5480..7213
                     /locus_tag="PKM27_17790"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNR09178.1"
                     /translation="MEILLGRLKKIDRLLITSFLPPMALSFMIALFVLTMQFFWLYID
                     EIMGKGIGLFQIIELISYLTVSFVPLALPMSVLLASVMVFGNLGERYELSSMKSAGIS
                     LIRIMRPLVVVTILVAGFSYLASNYIIPKANLKFFTRLNDIRRQKPTLALEEGIFNDD
                     FYGYSIYIGKKKNENIEDVIIYDQSKASDLILCTRAEKGRMYTAENGRRFVLELENGE
                     QVEEPTPRYLEGGRRNYPLMRTRFARYTKSFDLDEFAISATDESQYSQNQKMLSNRQL
                     LIQIDTIKKTIGDGERKVGLGISKLFNVSIQKQDSISVGPAPDSLDHPDSLAAPDRPD
                     TPDRQVQARPVRDSTSSQSISRTTLSSFKEQIAAMSVRMDSLQGNETGDTTLLKDSLT
                     AQSGKPRKDDPEVDLKPGLKVAHFYELLDSSRRSRWFRETGTAANLVQSEIINVLFSV
                     QNFKKKEILHWQQYYQKYTLALSCILFLFIGAPMGAIVRKGGFGYPLLIAIIFFIMYI
                     MASKLFEKLTDSMVLRVEIGMWMTTLIISAIGIYLTYRASKDMSSNILDVLTEKVVRL
                     FSRKKRLEVRG"
     gene            complement(7272..>9404)
                     /locus_tag="PKM27_17795"
     CDS             complement(7272..>9404)
                     /locus_tag="PKM27_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09179.1"
                     /translation="RRDEMLIATKVGFGRVAGPRVDRTTRGTYGMGILKSTDVGKSWK
                     QSLNLGSGLISGIQDMKFDPYDDYIIYAATTHGLYKSTNQGQSWSLIHNLPMAVDVEI
                     NPHNPEILFVSHGSFQDGATSGIYRSNNSGQTFTKLKNGLPATYSGKAKIDISKSNPD
                     IIYASVANAFKSIGLFMSIDGGNSWDLVNQKDVADVQGWYSHDIAIHSTDPDYIAYVG
                     QNAYISDNQGINFINVTSWDAGDMGRVPVGGPEGIGIYVHADIHAAYFHPKRPDEIYF
                     ATDGGIFVSTDKGMSFEGRNGGYVTTQFYANFSASRTDPEFAIGGMQDNGTAIYDGQD
                     AWIKVIGGDGMSTAINPFNENVIFGSFQYFGLNRSTDRGKTFEYLKPSHSIFLSEPKA
                     FNTPFEVVASSPNSMYAGAQKVYLNEGAGNPSLWKATHTSPLDPEATVLTLAVAPSDP
                     SIIFASTSPLNNNTVPKVFKSTDAGKQWTRLTNLPAKSAMDIAIDPTDARIVYIVFSG
                     FGAGTHVFKSFDGGTSWNIKENGLPDVPVNCVIIHPKNRNELFLGNDLGVFYSKNQGE
                     SWEKFMEGLPNAVMAMSLSIPSDAESIKLATHGNGVYESSLPLSLPVAELNNPFLKNF
                     SVSPNPAMVSAQARLHLEKPAKYRINIIDFSGKTLFDSPERQGQAGLNTTDLDLSPYA
                     SGTYLIGIRGHILENGSPFQRTVKLVKR"
BASE COUNT         2194 a         2350 c         2580 g         2280 t
ORIGIN      
        1 tatcattacc tcgacaagga aaaggtaagg ctggtcgctg tggaagccgc aggcgaaggc
       61 attcacagcg gccggagcgc agccacgagc atcctgggat cggtggggat catccacgga
      121 agcaaaacac tgctgatgca gactgcggat ggccagatca tcgaacccca ttccatttcc
      181 gccggcctgg attatccggg gatcggcccc ctgcacgccc acctgatccg gacgggccgc
      241 gcagaggtca tagccatcac ggatgaagag gccgtcaaag ccgcttttgt tttaacgagg
      301 ttggaaggca tcattccggc cctggaatcc gctcatgccc tggcggccct tgaccaaatg
      361 cagtaccggc cgggggaaaa tatcgtgatc tgcctttccg gccgtggaga caaggacctg
      421 gccacttaca tgaaatatct agatacctat gagtcgattg tttgatttat ttgcccggaa
      481 acaggggggc atcctcaata tctatttcac cgccggattt cccggcctcg aagatacggt
      541 ccccatcatc aaggccttgt ccgacgcggg tgcggacctg gtcgagattg gaatccccta
      601 cagcgatccg cttgccgacg ggcccaccat ccaggccagc ggccaggccg cgctcaaaaa
      661 cggaatgacc ctggacctgc tcttcagaca ggtcgcccag gccagggcct atacttccat
      721 gccatttata ctgatgggtt atttcaacca ggttttgcaa tttggagagg agaatttcct
      781 gtccagggcc aaagagagcg gggtagatgc gctgatcctg cccgacctgc cggtggagga
      841 atatgaattg cattaccggt ccctgtttgg gaaatacggc atggacatcg cctttctgat
      901 cacgccccag accccggaag cgcggatccg gcacatcgac caggtaagca acagttttat
      961 ctatatggtc tcttccaatg ccatcaccgg tgccaaagcg ggaatcgatg actaccagga
     1021 agcctatttc aggcggatat cggccatggg attaaaaaat ccaaggctta tcggcttcgg
     1081 tatatccaac aaggcgacct ttgaccaggc ctgccgtttt gcccagggcg ctatcatcgg
     1141 cagcgctttt atcaatgccc tggccggcag caaggatctt gcctcgacgg tttcgcaatt
     1201 tatcgggcag gtgaggcaag ggtagtgggg agagtcattc ttttttccca atcctaatca
     1261 aggagaaatg gagacaggag acgggaggaa gggaggaaag gttaacggaa aatggttaaa
     1321 ggttaaggga gcaagagttc cagggaacca cttaagctaa gcttttgtga gggaggaggg
     1381 ttaattttct tcttggtgaa ctgcgtatcg ttgtgggagc cctttttggt cagttccggg
     1441 tggaccttca aaccatacct tggccggggc cggatcttca cgcataatcg taaatttgca
     1501 tatgcaatta ctcgacggtg tcctggtggc aaaaaaaata aaggctgacc tccttcagtt
     1561 agtgaaagcg gagcaggaca aagggaaaag agttccacac cttgctgcgg tattgatcgg
     1621 tgatcatccg gcttccgcct cctatattaa aaacaagatc cggtcctgcg ctgaagccgg
     1681 ctatgaatcc actctgatcc acagggatag ttccatcacg aaggagggtt tgcttgaaat
     1741 cgtggacgga ctcaaccgca atgaggccat tgacggttac attgtgcaac tgcccctgcc
     1801 caggcatatc gacgaacagg aaatcctcct agccattgat cccaaaaagg acgtggacgg
     1861 cttccatcct atgaatatcg gccgcatggt gataggcctg ccggcttatg tgcccgccac
     1921 gcctctgggc atcctcaaac tgatcgaaca ttaccggata ccgaccgaag gaaaacacgt
     1981 ggtggtgatc gggaggagca atattgtagg taccccggtg tcgctcctgc tctcaagaaa
     2041 agcctacccc ggaaatgcca cggtcaccct ctgccattcc aaatcaaccg acctggcgga
     2101 aatgagccgg aaggcggata tcctgatcgc ggcgatcgga acgcctcatt ttgtgaaagc
     2161 ggatatgatc aaggatggcg ccgcggtgat cgacgtgggt atgaaccaaa ttgcggatcc
     2221 tgccagtaaa aagggccacc ggctggtagg ggatgtggac tttgaagggg tttcccacaa
     2281 ggccgcctac ctgactccgg tccccggcgg agtgggccct atgacggtat gcgccctgat
     2341 gatcaatacc tggacggcct ataccgggaa aatatatgga tgaaataaac caaaattatt
     2401 accgcctggt gtttacctcc ttaccggatg agatcaccct tgtgcattta tatgaatgcg
     2461 gcctggaggc cgttgaggaa aaagagacgc atatcgaagg ttatctgccc gaaagtcttt
     2521 acagcccggc cttgctggac aagctccgtt ccatccttcc catccggacc tcggagcgac
     2581 tggagccaca aaactggaat gcgatctggg aatcctcttt tcaaccggtc tgggtggacg
     2641 gaaaggtgca tatccgggct tcctttcatg aaaaaagccc cggggggatg gatgtcgtga
     2701 tcgatcccaa aatggccttc ggcacggggc atcatgccac caccagcatg gtcatccggg
     2761 aaatgctgga tctcgatctg gaaggccggg ccgttctcga cttcggctgc ggcagcgggc
     2821 tcctgtcgat cgtggccgaa aaactcgggg ccgggcgcat caaagccctg gactatgatc
     2881 cctggtccgt cgagaatacc aaggaaaata tgaaggtcaa ccaaagccgc catattgaag
     2941 tgaagcaggc cgatagcctg aatgaagaaa cgggggctta tgatctcatt ttggccaata
     3001 tcacccgcga tgtccttatt cggaataccc cggatatcag ccgcctgctc aaaccagggg
     3061 ggcatgccgt ttattccgga ttcttatccg gggataggcc catcctggag accgaaatcc
     3121 gcaagtacgg gatggaaccc ggaaaatccg ccgaagaatc aggttggggc gtcctggtca
     3181 tgaccaaaac ctgaagcaaa aaaaaagccc gtgcggaaag catgggcttt ttttgtgctg
     3241 tatttttttt aaatgatttc ttctttctcg aaagaacgca acattttgga aatatcgtcc
     3301 tgagtaaaat gtcccctgag gcttaccagg accgattcat cgtgtttttc atggatcagg
     3361 aagaccactc ttctcagaat gtcttttttt tgccaggcga aaacctccac cctcgaaccg
     3421 ttgtccctca aagaaaccaa tggttcatag ttctccgcca ccaggttctt tttcagggtg
     3481 cggacatcct cgtcgcggaa accctcgcct ccatcggaaa acatcacgga gatgctgccc
     3541 aaattgcgga ccaggggcct catcgcctcc ctttgccatt ggtcttcctc gcggagggcg
     3601 atgccgcccg cgagccggac cagaaaaccc gggatggaaa aatggcggat cccatcccgg
     3661 ttctgattcg cgcgtacaaa ctggttgaga ctttgttcct gggccgtgga cagggcggat
     3721 aaacacaggg tgcaaaacaa actaaaaatc aaagtcttca tggtcagttt tttttctgga
     3781 ttttttcaag atgttcagag ccttcgatat tgagggtccg gcccagccgt cctaccttgc
     3841 tcaggtcaat gtctccgaca aagctgacca gggtaaattc atccggaccg ccgacgagca
     3901 tcagcaattc gtcgatctta tccgggttgg ccgcattgct tttggtcagg aagagcacgt
     3961 cctcgccttt gtcccggact ttcatcagtt cttcgtattc ctgggtgttg atcttcccga
     4021 ccgcttcctt gtagaaagcc tgtggagaat cttcggtgct gaggatatgc aggcccttga
     4081 tggtctggag cagggacctc aattcagggt ccatatcatc cagtttgatc ttcgagacca
     4141 attggaacat tttaggactg atgtagacgg aggtaaaccg ttcatcgtcc tggtaggctt
     4201 taaaaaagcg cgtgacagcg tccgactggg cggttgccag aatggtgctg aacaaaaggg
     4261 gtaagaaaat aaagtttttc atggttatga tagatttcaa aagattcagt ttaaaatgtc
     4321 caggttttcg gtcttttcga gggacttggt cgtctggtcc gccacccggt tcatgcggtt
     4381 tgagaggtat aaaagggccg ccttggtttg ttcgagggct tcttcggggg attcaaaagt
     4441 atcccggatc gccagctggt ctttttggga atgctggtag cggtaggtgc cgatgcccgc
     4501 cgtgaccagc aggatcaggg aagccgcgac ggccagggcg cgcttccagg tgagccggac
     4561 gattttggtt tcggagcgcg gatggagtat cctgtccatg atctggtctt ccagatctat
     4621 ggtcacctgg cgttcctggt caaaataatc gaaaaggggc tggaaggctt taaaggcttc
     4681 atccacctgg ccagcgtgga aataggccgc caggtttttt tcatcctcga gggtggactc
     4741 accggcccag tatttatcca ggagttgttt gatatattct ttagagttgt tcatataatc
     4801 ccgattgtat aagtttttcc tttagattaa gcctggcgcg gtgcaggttg actttcacct
     4861 gggcaaggga aagcgaacaa gcctccgcga tttcatcgta ggacaaacct tctatatccc
     4921 gcaggcgaag caccatcttt tgtttttcgg gcatttcgcc gatcagtttt ttaatcaggt
     4981 tcatcacttg ggcttttgcg gtcttctcat gtgggttgtc ctggctgtcc tggacctgta
     5041 cctgggtacc tatatcgagg tggtttttcc gggcccggat ccggtcgata cacaggttcc
     5101 gggtgacggt gaacatccat ccctccaggt tttcgatgcg atcgagttcg tcgcgccggt
     5161 cccagatttt gatcaaaaca tcctgtacaa tgtccttggc gtcttcttct ttttgtaaaa
     5221 ggtgcagggc gtaccggaaa atcttgcttt gattggcctt tacaaactgg cggaattcct
     5281 gtggatccat gtacgagggg ttgacggctc ttaacaaata ttggttacag acccgggcaa
     5341 aaatgtttcc gatgtcccgg ttttcggtgg gtaaggccca aaattaagtt aaaatgcaat
     5401 gaattggcgg acttgaggca ttctgcccgg aatatccttt tacccccccg ctggcgccgg
     5461 aagtcttaat tttgccggga tggagatctt gctgggccgg cttaaaaaga ttgatcgttt
     5521 actgatcact tcttttttgc ccccgatggc cctgtccttc atgatcgcgc tttttgtgct
     5581 caccatgcag tttttctggc tgtatatcga tgagatcatg ggcaagggca tcgggctgtt
     5641 tcagatcatc gagttgatca gttatctcac cgtctccttt gtccccctgg ctctcccgat
     5701 gtcggttctc ctggcctcgg tgatggtctt cggcaacctg ggggaacgat atgaattgtc
     5761 gagtatgaaa tcggccggga tcagcctgat tcggatcatg aggcccctgg tggtggtcac
     5821 catactggtc gccggcttct cttatttagc ttccaattac atcattccca aggcgaatct
     5881 taaatttttt acccggctca acgatatccg gaggcaaaaa cccacccttg ccctggaaga
     5941 aggcattttc aacgacgatt tttacggtta ttccatctat atcggtaaaa agaagaatga
     6001 gaatatcgag gatgtgatca tttatgacca gagtaaggcc agcgatctga tcctatgcac
     6061 ccgggcggaa aaaggaagga tgtacaccgc agaaaacggc aggcgttttg tacttgaact
     6121 cgaaaacggc gaacaggtgg aggaacccac cccgcgatac ctggaaggag gccggcgcaa
     6181 ctatcccctg atgcgcaccc gctttgcccg ctataccaag tcctttgacc tggatgaatt
     6241 tgcgatcagc gccacggatg aaagccagta ttcccaaaac caaaaaatgc tgagcaaccg
     6301 tcaactgctg atccagatcg ataccatcaa aaagacgatc ggggacggcg aacgaaaagt
     6361 gggtctgggt atctccaaac tgtttaatgt gagcatccaa aaacaggaca gcatatcagt
     6421 gggaccggcc ccagatagcc tggatcaccc cgatagcctg gctgcgccgg accgcccgga
     6481 tacccctgac cggcaggttc aggctcgacc cgtccgggac agtacatctt cacagtccat
     6541 ttccaggacc accttaagca gtttcaagga acaaatcgcg gctatgagcg tccggatgga
     6601 cagcctgcag ggaaacgaaa cgggggatac cacgcttctg aaggacagcc tgacggcgca
     6661 aagcgggaaa ccacgaaagg atgatccgga agttgacctc aaaccgggat taaaagtcgc
     6721 ccatttttac gagttgctgg acagcagccg gaggtcgagg tggttcaggg aaaccggtac
     6781 ggccgccaac ctggtgcaat ccgaaatcat caatgtcctt ttttcggtgc agaactttaa
     6841 gaaaaaggaa atcctgcact ggcagcaata ttaccagaaa tacaccctcg ccctttcctg
     6901 cattctgttt ctattcattg gggcgccgat gggcgctatt gttcgtaagg ggggatttgg
     6961 ctaccccctc ctcatcgcca tcatcttttt tatcatgtac atcatggcgt ccaaactttt
     7021 tgagaagctc accgactcca tggtgctccg ggtggagatc ggcatgtgga tgaccacgct
     7081 gatcatttcc gccattggaa tctatctgac ataccgggcc agcaaagaca tgtcttcgaa
     7141 tatactggat gtgttgacgg agaaggtggt aaggttgttt tcgaggaaaa agaggttaga
     7201 ggttagaggt tagaggtttg attgaggttt gaggtttgag gtttgaggtt tgaggtttga
     7261 ggggaaatac cttatctctt caccaatttc acggtccttt ggaaaggcga tccattctcc
     7321 aggatatggc cgcggatacc tattaaatag gtgccggatg catagggcga aagatcgaga
     7381 tcggttgtat tgaggccggc ctggccttga cgttcagggc tgtcgaagag ggttttgccg
     7441 ctgaagtcaa tgatgttgat tctatatttg gccggttttt ccaggtggag cctggcctgt
     7501 gcactaacca tggcgggatt ggggctgacc gaaaagttct tcaggaaagg attattgagt
     7561 tcggccacag gcagggaaag aggcaggctg ctttcgtaga ccccattgcc atgggtcgcc
     7621 agcttaatgc tttccgcatc tgatggaatg ctcaggctca tggccatgac ggcattggga
     7681 aggccttcca taaatttttc ccaggactcc ccctggtttt tggaataaaa cacaccaagg
     7741 tcgttaccga ggaacaattc attccggttt ttcggatgga tgatgacgca attgaccggt
     7801 acgtccggca ggccgttttc cttgatattc caggaagtac cgccatcaaa ggatttgaat
     7861 acatgggtgc ctgcgccgaa tcccgaaaag acgatataaa cgatccgggc atccgtcgga
     7921 tcaatggcaa tatccatggc ggatttagcg ggcaggttgg tcaggcgggt ccattgttta
     7981 ccggcatcgg tgcttttgaa taccttgggt acggtattgt tgttgagcgg ggaggtgcta
     8041 gcaaaaataa tcgaagggtc agaaggggcc actgcgagcg taaggacggt ggcttcaggg
     8101 tccagggggc tggtatgcgt ggctttccat aaactggggt tgccggcgcc ctcattcaaa
     8161 tatactttct gggcgcctgc atacatggaa ttcggggagg aggcgacgac ttcaaagggc
     8221 gtattgaacg ccttaggttc ggatagaaat atcgaatggg atggtttgag gtattcaaag
     8281 gtcttgcccc ggtcggtgga tcggttcaat ccgaaatact gaaaggaacc aaagatcacg
     8341 ttttcattaa agggattgat ggccgtggac atcccgtcgc ccccgatcac tttaatccag
     8401 gcatcctgcc catcgtaaat ggccgtgccg ttgtcctgca tccccccaat ggcaaactcg
     8461 gggtcggtcc tggaagcgga aaaattggca tagaactggg tggtgacata tcctccgttg
     8521 cggccttcaa aactcatgcc cttgtccgtg gaaacaaaaa tccctccgtc tgtggcaaaa
     8581 taaatttcat cggggcgttt gggatggaaa taggccgcgt ggatgtctgc atgaacgtat
     8641 atccctatgc cttccggccc gccgacgggt acgcggccca tgtctcccgc atcccaggag
     8701 gtgacattga taaaattgat cccctggttg tcggagatgt aggcattttg ccccacgtag
     8761 gcaatgtaat ccggatccgt gctgtgaatg gctatatcgt gggaatacca gccctggaca
     8821 tcggccacgt ctttttggtt gaccaggtcc cagctgttgc ccccgtcgat gctcataaaa
     8881 aggccgatgg acttgaacgc attggccacg gaggcataga tgatatccgg attgcttttg
     8941 ctgatgtcga ttttggcttt gcccgaataa gtggcgggca ggccgttttt aagtttggta
     9001 aaggtctggc cagaattgtt tgaacggtaa atgccgctcg tggcgccatc ctggaaactt
     9061 ccgtgggaga caaaaaggat ctcgggatta tgcggattga tctccacatc caccgccatg
     9121 ggcagattgt ggatcagcga ccagctttgg ccctggttgg tggatttata caaaccatgg
     9181 gtggtggcgg catagatgat ataatcgtca taagggtcaa atttcatatc ctggattccg
     9241 ctgatcaggc cggatcccag gttgaggctt tgtttccagc ttttgcccac gtcggtggat
     9301 ttgaggatac ccatgccgta agtgccgcgg gtggtgcggt cgacgcgagg gccggcgacg
     9361 cgcccgaagc ccaccttcgt cgcgatcagc atctcgtcgc gacg
//
LOCUS       DAOQXT010000148         9196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_8024_length_9196_cov_5.075046, whole genome shotgun sequence.
ACCESSION   DAOQXT010000148 DAOQXT010000000
VERSION     DAOQXT010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8024_length_9196_cov_5.075046"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1053)
                     /locus_tag="PKM27_17800"
     CDS             complement(310..1053)
                     /locus_tag="PKM27_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNR09180.1"
                     /translation="MKLTLKVWRQADPQAKGRFETFSVNANEHMSFLEMFDVLNEELL
                     AEKKEPIAFDHDCREGICGACSMVINGQAHGPLGGTTTCQLHMRHFKDGDIIVVEPFR
                     ASAFPVIKDLVVDRSSFDRIIQAGGFISVNTGQAPDGNAIPVERDKAGAAFDAAACIG
                     CGACVAACPNASAMLFTAAKVSHLALLPQGQIERKTRVKNMVNQMDIEGFGKCSNTYA
                     CEAECPKEISVDYIARMNREYFAAVIAGE"
     gene            complement(1177..3171)
                     /locus_tag="PKM27_17805"
     CDS             complement(1177..3171)
                     /locus_tag="PKM27_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNR09181.1"
                     /translation="MELDSKIPPGPIAEKWTRFRSTVPLVAPQNKRRLEVIVVGTGLA
                     GASAAASLGELGYHVKCFTFHDSPRRAHSIAAQGGINAAKNYANDGDSIFRLFYDTIK
                     GGDYRAREANVHRMAEVSVNIIDQCVAQGVPFAREYGGLLENRSFGGVQVSRTFYARG
                     QTGQQLLLGAYSALSRQISLGSVKMYNRHEMMDLVLVDGKARGIIVRNLVTGKLERFG
                     AHAVILATGGYGNVYYLSTNAMGSNVSAAWKAVKRGALMANPCFTQIHPTCIPVSGDY
                     QSKLTLMSESLRNDGRVWVPKKMEDVRAIREKKKKATDIPEDDRDYYLERMYPSFGNL
                     VPRDVASRAAKKVCDEGRGVNATGLAVYLDFASAIERFGKSQANVKGIQNPDAATIRK
                     LGEAVISEKYGNLFEMYEKIVGEDPYKTPMMIYPAIHYTMGGLWVDYNLETNIPGLFA
                     LGECNFSDHGANRLGASALMQGLADGYFVIPYTIGTFLSKEIRTPATDTASPEFDKVE
                     KDVRDRIDHLLSVQGKQSVESLHRQLGKIMWDHCGMARNREGLEQARIQIRQLRDTFW
                     KEVYVPGKALEFNPELDKANRVADFMELGEMLVVDALDRNESCGGHFREEYQDSEGEA
                     LRRDDLYAYVSAWAWNDSNMELTKEALKFENVELKSRSYK"
     gene            complement(3179..3859)
                     /locus_tag="PKM27_17810"
     CDS             complement(3179..3859)
                     /locus_tag="PKM27_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNR09182.1"
                     /translation="MNWMVPFLKSSLGRKLIMSLTGLFLCLFLVVHLAGNFQLLKSDG
                     GEAFNTYTYFMTHNPLIKLISYFTYFFILLHTFQGLVLYFHNRSARGAAYLDKHPSKT
                     TFASRNMALLGSVIFLFLIVHMGDFWFKMKRGILAKVSYEGYDQIDDLYTRVTAAFDQ
                     WWIVLFYVAGMGFLGFHLQHGFQSAFQSLGLNHPKYTPWIKNTGIVFCILISAGFAII
                     PIIQYFSK"
     gene            4046..4546
                     /locus_tag="PKM27_17815"
     CDS             4046..4546
                     /locus_tag="PKM27_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09183.1"
                     /translation="MSSPLFQQFIDYFPTVELPVTLTLDSHRDFEEVNVLLPAAMSAA
                     FISSEEEMPGEEFTEYLPCFRLPGDQEFISLVYWKATLLRHDFIIATYTKSGIPISRQ
                     VIAGTSSDGKTIVKKVATLQPDDSIDVIASHQEADHIPFDPSQTQELNYELMPNGFIA
                     LLNENQ"
     gene            4533..6731
                     /locus_tag="PKM27_17820"
     CDS             4533..6731
                     /locus_tag="PKM27_17820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HNR09184.1"
                     /translation="MKTNNEKNKPGKKHHFDALKELTAGHLKKNPRKSYSARQLLKAI
                     QAGNDKSDIEQVLSRLVQTRRVTEVRKGQFQYGQGSGNRSSRGKTSGNGDIAIEGRLE
                     VIRSGAAYLLSDQSAQDVYIPAKYLQGAIHGDIVKVQVEGGRKKRPEGRVVQILKRET
                     HYFVGTFHHRRNIHIVYPDSEHHRLEIHVRNQDSMDARDGDKVIVHILAWRDEAHRFP
                     TGKVTQVLRADALIDNEMMGTLINHGFPLVFSDEALEIARKAAEGMQDGLAWPDRRDF
                     RQVTTFTIDPLTAKDFDDAISFQRLDNGHLEIGIHIADASHYLEEGTVLDKEAYAKST
                     SVYLVDRALPMLPEVLSSDICSLNPLCDRLTFSVVFTLDPSHYKITGTWIGKSVIHSN
                     HRFVYEDAQEVLDRGDGIFHEELNLLNKIAVHFRKKRFAEGSIGFESPEYRFDLDPVS
                     GKPVSVREKTRIDTHILVEEYMLMANRAVAEFINNKEKSQDSIPFVYRVHDLPDLDKL
                     SDLALFAKEMGFELRMNTPKEITRSLNALYEASATNDSLKILQPLAIRTMAKAEYSTN
                     NIGHYGLAFDLYSHFTSPIRRYSDILAHRILAKNLNQTFRTDKKRLEEQCKYISNQER
                     KAMEAERDSVKFFQLLYIADHVGQEFTGKISGLIERGIFTELDDNHVEGFIAFEEMDE
                     PFILAASRLKVMGRRSGTVLSMGDRIKVRIESAELSSKRVQMAFVGKVSQ"
     gene            6882..8141
                     /locus_tag="PKM27_17825"
     CDS             6882..8141
                     /locus_tag="PKM27_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HNR09185.1"
                     /translation="MMLSIFPLKNDAATFGVLISVLALIFWTTQSQRPFWKKFYTYVP
                     ALLLCYFIPALLNWPLGIITNENSKLYPFVSRYLLPASLLLFCISLDLKAIARLGPKA
                     LLVFLAGTVGVMIGGPLAFLVMVRFFPGFLPAPPSELWAGLATIGGSWIGGGANQTAV
                     KEIFQVPDSLFGTVVVVDIAVASLWMAILLYGAGIHHRINRWLKADFSQIDALRVKME
                     AFSASVARKPDLTQLMILMGLTFAGVGLAHWLTDGIIPFMNERQEWLDRYKLGAINSS
                     FFWIVVLSTSIGIALSFTRARNLEGVGASTWASIFLYLLVATIGMQMNLGEVAQNLGL
                     ITIGVLWMLFHVAVILLVARLLHAPFFYVAVGSMANIGGAASAPVVAAAFGPGLAPVG
                     VLLAVLGYAIGTYGGLVVAYLMQGIAG"
     gene            8279..9088
                     /locus_tag="PKM27_17830"
     CDS             8279..9088
                     /locus_tag="PKM27_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNR09186.1"
                     /translation="MEKKRILFVTQEMKPYTELGEMSDIVNQITQYVSANGYEIRVLM
                     PRFGTINERRHRLHEVVRLSGMNIIVDDDDFPLIIKVASLPGSRMQVYFLDNEEFFKR
                     KYIFKDAAEAQFDDNLDRMVFYSKGSIETVKKFGWVPDIIHCHGWMTSLIPLYLRTTY
                     KDEPIFRNAKIVYSVYNDSLDMPFDKKFITKASINNLDPKKLDDFYSGKHYRLHQGAM
                     KYADALISGSEKLDKEVITAKKSLGKKPQLDYLPEEGRNQAYLHFYETLLK"
BASE COUNT         2206 a         2466 c         2292 g         2232 t
ORIGIN      
        1 aacttgccaa cttgctaact taaatttctg cccacctacg cacacccctg tttcccctga
       61 aggggagctc ccgcacggac tacagaataa gcagtataga attgcataag cactttacta
      121 aactggctga ctggcaaact ggttaactgg ctaactggcc aactaatctg aataaataag
      181 tgtaaacggt aaagggttaa gggttaaagg ttaaaggacc aaaattacca aatgaccacc
      241 gactactgac tactgactac tgactattga ctactgacta ttgactattg actattgact
      301 attgactaac tactcccccg caattaccgc cgcaaaatac tccctgttca tccttgcgat
      361 ataatccacc gatatttcct tcgggcactc cgcctcgcag gcataggtgt tgctgcattt
      421 gccaaagcct tcgatatcca tctggtttac catgtttttt acccgggtct tcctttcgat
      481 ctgtccctgg gggagcaggg caaggtgaga gaccttggcc gcggtaaaga gcatggcgga
      541 ggcgttgggg caggcggcga cacaggcccc gcatccgatg caggcggcgg catcgaaagc
      601 ggctccggcc ttgtcccttt ccaccggaat ggcatttccg tccggcgcct ggcccgtatt
      661 cacagatatg aatcctccgg cctggatgat ccggtcgaag gacgaacggt ccaccaccag
      721 gtccttgatc accggaaaag cgctggcccg gaaaggttcg accacgatga tgtccccgtc
      781 cttgaaatga cgcatatgga gctggcaggt agttgtcccg cccagggggc catgggcctg
      841 gccattgatg accatagaac aggccccgca aatcccttcc ctgcaatcgt gatcaaaggc
      901 gatgggttcc tttttttcag ccagcaattc ttcattgagc acgtcaaaca tttcgaggaa
      961 agacatgtgt tcattggcat tcaccgaaaa ggtttcaaat cttccctttg cctgagggtc
     1021 tgcctgtctc cagactttta atgttaattt cataaaaaac agatttcagc gatcagcttt
     1081 cagatttcag caatcagctt tcagcttgca gcttgcattt cgggaaatat cctgtttgcc
     1141 ggctgaggtc tgacagctga cagctgatag ctgttcctat ttataacttc tagattttaa
     1201 ttccacattt tcaaatttca gggcttcttt ggtcaattcc atattgctgt cgttccaggc
     1261 ccaggctgaa acataggcgt acagatcgtc gcggcgcagg gcctcccctt cactgtcctg
     1321 gtattcctcc cggaaatggc caccgcaact ctcattgcgg tccagggcgt ccaccacgag
     1381 catttcgccc agttccataa aatctgccac ccggttggct ttgtccagtt cgggattaaa
     1441 ttccagggct ttgccgggta catagacctc tttccagaac gtgtcacgca gctggcggat
     1501 ctgaatacgc gcctgttcca ggccttcccg gttgcgggcc attccgcaat gatcccacat
     1561 gattttaccg agttgtcggt gcaggctctc cacggactgt ttgccctgga cactcagtaa
     1621 atggtcgatg cggtcacgga catccttttc caccttatcg aattccgggc tggccgtatc
     1681 ggttgccgga gtgcgtattt ccttcgataa aaaggtcccg atggtatagg ggatcacgaa
     1741 ataaccgtca gccagtccct gcatcagggc cgaggccccc agccggttgg ctccgtgatc
     1801 actgaaatta cattctccca gggcaaacaa tcccgggata ttggtctcca ggttataatc
     1861 cacccaaagg cctcccatgg tatagtgtat ggccgggtaa atcatcatgg gtgttttata
     1921 aggatcctcc cctacgattt tctcatacat ctcaaacagg ttgccgtatt tttcactgat
     1981 cacggcttcc cccagtttac ggatcgtggc ggcatcgggg ttttgaatgc ctttcacgtt
     2041 tgcctgtgat ttcccgaaac gttcgatggc cgaagcaaaa tcgaggtaaa ccgccaggcc
     2101 ggtggcatta accccacggc cttcgtcgca tacttttttg gctgcccggg aagccacgtc
     2161 ccggggcacc aggttgccga aggagggata catccgttcg aggtaatagt cccggtcatc
     2221 ttcgggaata tccgtcgctt ttttcttctt ttcccggatc gcgcgcacgt cctccatttt
     2281 tttaggcacc catacccggc cgtcgttgcg tagggattcg gacatcaggg tcagcttgga
     2341 ctggtaatcc ccgctcaccg gaatacaggt ggggtggatc tgggtaaaac agggattggc
     2401 catcagggct ccccgcttta ccgctttcca ggccgcgctc acattggagc ccatggcatt
     2461 ggtcgaaaga taatagacgt tgccgtagcc cccggtggcc aggatcacgg catgggcgcc
     2521 gaaacgttcc aatttgccgg taaccaggtt gcgcacgatg atgccccttg cttttccgtc
     2581 cacgaggacg aggtccatca tttcgtggcg gttgtacatc ttcacgctac ccagtgaaat
     2641 ctggcgcgaa agcgcgctgt aggcgccgag gagcaattgt tgcccggtct gccccctcgc
     2701 gtaaaaagtc cgcgatacct gcacgccgcc gaaggaccgg ttttcgagca aaccgccgta
     2761 ttcccgtgca aagggcaccc cctgggcgac acactggtca atgatgttga cactcacttc
     2821 cgccatacgg tggacattgg cttccctggc ccggtagtcg ccgcccttga tggtatcgta
     2881 aaacaaacgg aagatgctgt ccccgtcgtt ggcataattt tttgccgcat tgatcccgcc
     2941 ctgcgccgca atgctgtgcg cccgcctggg gctgtcgtgg aaagtgaaac attttacgtg
     3001 atagcccagc tctccaaggc tggctgccgc cgaagcgcct gccaggccgg taccgacgac
     3061 tatcacttcc agcctcctct tgttctgggg agccaccagg ggcacggtcg atctgaaccg
     3121 ggtccatttt tccgcgatgg gcccgggggg aattttggaa tccaattcca tatatttttt
     3181 atttagagaa atattgtata atcgggatga tggcaaaacc tgccgaaatg aggatgcaga
     3241 agacgatacc cgtgtttttg atccatggag tgtatttggg atggttcaaa cccagggact
     3301 ggaaagcgct ctgaaatccg tgttgcaggt ggaagcccag gaaacccatg ccggcgacat
     3361 agaacagcac gatccaccac tggtcgaaag ctgcggtgac ccgggtgtac aaatcatcga
     3421 tttgatcgta accctcataa ctgaccttgg ccagaatgcc ccgcttcatc ttaaaccaga
     3481 aatcccccat atggacgatc aggaaaagga agatcacact gcccagcaag gccatgttcc
     3541 ttgatgcgaa agtggttttg gaagggtgtt tgtccaggta agcggcgcct ctcgccgacc
     3601 ggttgtgaaa atacaggacc aggccctgga aggtgtgcaa caatataaaa aaataggtga
     3661 agtagctgat cagcttgatc agggggttgt gggtcatgaa gtaggtatag gtattaaaag
     3721 cttctccccc atccgatttc agcaattgaa aattgccggc aaggtggacc accaggaata
     3781 aacataaaaa caaccccgta aggctcataa tcagtttcct gcccagggag gattttaaaa
     3841 agggaaccat ccaattcata attctgtctg tgacgctttt ttccgaggtc aaaaataaca
     3901 aagccgttcc tcgtctgctt tatccggctc gcggtaaata tatttagaaa tggtctaaac
     3961 taaaaactat ccttgacttt gctttgttta gaggaagtcc gggtccgcag ccggttaaat
     4021 attttaaaat taactttgca gatgaatgag cagtccctta tttcaacaat ttattgatta
     4081 ttttccaacg gttgagcttc cggtcaccct taccctggac agccaccggg actttgaaga
     4141 agtgaacgtc ttgcttcccg cagccatgtc cgccgccttt atttccagtg aagaagaaat
     4201 gccgggggag gaatttacgg aatacctgcc ctgcttccgc ttgccggggg atcaggaatt
     4261 tatatccctg gtctattgga aagcgaccct gcttcgccac gattttatca tagccaccta
     4321 taccaagtcg ggcatcccga tctccaggca ggtcatagct ggaacctcca gcgacggaaa
     4381 aaccatagta aaaaaagtag ccacccttca acccgacgac agtattgatg tcattgcttc
     4441 ccaccaggaa gcggaccaca tcccctttga tcccagtcag acccaggaac tgaattacga
     4501 gttgatgccg aacggcttta ttgcattatt aaatgaaaac caataacgaa aaaaacaaac
     4561 cgggcaagaa gcaccacttt gacgccctta aagaattaac agccggtcat cttaaaaaaa
     4621 atcccagaaa atcgtattcc gcgcgccagc tgctcaaagc catccaggcc ggcaatgaca
     4681 agagcgacat agaacaggta ctcagccggc tggttcagac ccggagggtg accgaagtcc
     4741 gtaaaggcca gtttcaatac ggccagggtt ccggaaaccg gtcgtccagg ggcaaaacat
     4801 ccggaaacgg ggacatcgcc atcgaaggaa ggctggaagt catccgcagc ggcgccgcct
     4861 atctcctctc ggaccagagt gcgcaggatg tttatatccc cgccaaatac ctccagggcg
     4921 cgatacacgg cgatattgtg aaagtgcagg tggagggagg ccgcaaaaaa aggccggaag
     4981 gcagggtggt tcaaattctc aaaagggaaa cgcactattt tgtcgggacc ttccatcacc
     5041 gccggaatat ccatatcgtc tatccggaca gcgaacacca ccgcctggaa atccatgtca
     5101 ggaaccagga cagcatggat gcccgcgacg gagataaagt catcgtccac atcctggcct
     5161 ggagggatga ggcacaccgg ttccccaccg gcaaggtcac ccaggtcctg cgtgcggacg
     5221 ccctgatcga caacgagatg atggggacct tgatcaatca tggctttccg ctggttttca
     5281 gcgacgaggc ccttgagatt gcccggaaag cggccgaagg gatgcaggac ggcctggcct
     5341 ggcccgaccg gagagacttc cgccaggtga ccacctttac cattgatcca ttgaccgcca
     5401 aggactttga cgacgccatt tcctttcaac ggctggataa cggccatctg gaaatcggca
     5461 ttcacattgc agatgcctcg cattacctgg aagaaggaac cgttctggac aaagaggcct
     5521 atgctaaaag cacttcggtc tatctggttg accgggccct gcccatgttg cctgaagtat
     5581 tgtcctcaga catttgttcc ctgaatccgc tctgcgaccg gctcactttt tcggtcgttt
     5641 ttaccctgga tccttcccat tataaaatca caggcacctg gatcgggaag tcggtgattc
     5701 actccaacca ccggtttgtc tatgaagacg cccaggaggt tctggaccgg ggggacggaa
     5761 ttttccatga agagctgaac ctcctgaata aaatagcggt ccacttcagg aaaaaaaggt
     5821 ttgccgaagg cagcattggt ttcgaatcgc ctgaataccg ctttgacctc gacccggtga
     5881 gcggaaagcc ggtttcggtc cgcgaaaaaa cccggatcga cacccatatt ctggtggaag
     5941 aatatatgct gatggccaac cgggccgtgg cagagtttat taacaataaa gaaaaatccc
     6001 aggactccat accctttgta taccgggtgc acgacttgcc tgacctggat aaattgtccg
     6061 acctggcctt gtttgccaaa gaaatgggat tcgaactccg gatgaatacc ccgaaggaaa
     6121 ttacgcgttc actcaacgcc ctgtacgagg ccagcgcgac caatgattcc ctgaaaatcc
     6181 tgcagcccct ggccattcgc accatggcca aagccgaata ttctacaaat aacatcggcc
     6241 actacggact tgcttttgat ttgtactctc actttacttc cccgatcagg cggtattccg
     6301 atatcctggc ccatcgcatc cttgctaaaa acctgaacca aaccttccgg accgataaaa
     6361 aaaggctgga ggaacaatgt aaatatattt caaaccagga acgtaaagcc atggaagcgg
     6421 aacgcgactc ggtgaaattt ttccagttgc tgtacatcgc cgaccacgtc gggcaggaat
     6481 tcaccgggaa gatctccggc ctgatcgaac gcggtatttt taccgaactg gatgacaacc
     6541 acgtggaagg atttatcgct tttgaggaaa tggatgagcc ctttattctg gcggccagcc
     6601 gcttaaaagt catgggacga aggagcggga ccgtcttgtc catgggtgac cggataaaag
     6661 tcaggattga atcggcggag ctttcatcca aaagggtgca aatggctttt gtgggaaagg
     6721 tgagtcaata gtcaagagtc aagagtcaag agtctatctc gcttctcgta tctcgaatct
     6781 cgcttctcgt atctcgcttc tcgtatctcg cttctcgtat ctcgtatctg gattctggga
     6841 gatgatctta attcccaata cataattacc ttgtggcgac catgatgctt tccatctttc
     6901 cattgaagaa tgacgcggcc actttcgggg tgttgatatc agtcctggcc ctgatttttt
     6961 ggactaccca aagccaaagg ccgttctgga agaagtttta cacttatgtg ccggccctgc
     7021 tgttgtgcta ttttattccg gcgctgctca actggcccct gggtatcatc accaatgaaa
     7081 actcaaaact ctatccattc gtttcccgtt acctcttgcc ggccagtttg ttgttgtttt
     7141 gcatcagcct ggaccttaag gctatagcca ggctcggccc taaagccctc ctggtattcc
     7201 tggccggaac agtgggagtc atgatcggcg ggcccctggc cttcctggtc atggtcaggt
     7261 ttttcccggg attcctgccg gcgccccctt cggaattgtg ggccggcctg gccaccatcg
     7321 ggggaagctg gattggaggc ggcgccaatc aaacggccgt caaggagatt ttccaggtcc
     7381 cggattccct gtttggcacc gtagtggtgg tggatatcgc ggtggcaagc ctttggatgg
     7441 ccattctatt gtacggagcc ggcattcatc accggatcaa ccgctggctg aaggctgatt
     7501 tttcccagat cgatgccctg agggtcaaaa tggaagcctt cagtgcaagc gtcgctcgaa
     7561 aaccggacct gacccagttg atgatcctca tgggactgac ttttgcaggg gtcggcctgg
     7621 cgcattggct cacggatggc atcattccgt ttatgaatga acggcaggaa tggctcgacc
     7681 gatacaaact cggggccatc aactcttcct ttttctggat cgtggtcctg tccacttcca
     7741 tcgggatcgc cctttccttt acccgtgccc ggaacctgga aggcgtcggg gcttcgacct
     7801 gggcatccat attcctatac ctccttgtgg ccaccattgg gatgcaaatg aacctggggg
     7861 aagtagccca gaacctggga ctgatcacca tcggcgtgct ttggatgcta tttcatgtgg
     7921 ccgtcatcct tttggttgcc cgcttgctgc acgccccgtt tttttatgtg gcggttggaa
     7981 gtatggcaaa tattgggggg gccgcctctg cccccgtggt cgcggccgca tttgggcccg
     8041 gcttggcgcc ggtcggtgtg ctcctggccg tgctgggtta tgccatcggt acctatggcg
     8101 gcctggtcgt ggcttacctc atgcagggca ttgcaggata aaatttgcct gaccggggga
     8161 tagactgctg taaaacaatc agatatcccg gcaaatgggc tttttcagcc cccggattac
     8221 cataaaatcc gtacctttgc catccgaaaa agaataagta tctcgttttc gtctttatat
     8281 ggagaaaaaa agaatccttt ttgtcaccca ggaaatgaag ccttataccg aactgggtga
     8341 aatgtcagac attgtcaacc agatcaccca gtacgtttct gccaatggat atgaaatccg
     8401 ggtcttaatg cccaggttcg gcacgatcaa tgaaagaagg caccggttgc atgaagtggt
     8461 tcgcctttcc ggcatgaaca tcatcgtcga cgatgatgat tttcccctga ttatcaaggt
     8521 ggcctccctg ccgggttccc ggatgcaggt ttattttttg gacaatgagg agttttttaa
     8581 acgcaaatat attttcaagg atgccgcgga agcccagttt gacgacaacc tcgaccgcat
     8641 ggttttttac agcaaaggat ccatcgaaac cgttaaaaaa ttcggctggg taccggacat
     8701 catccattgc cacgggtgga tgacctccct catccccctg tatctgcgca ccacttataa
     8761 ggatgaaccg attttcagga atgccaaaat cgtgtattcg gtttacaatg acagcctgga
     8821 catgcctttt gacaaaaaat tcatcaccaa ggcatccatc aataacctgg atcccaaaaa
     8881 actggatgat ttttattcgg gaaaacacta ccggctccac cagggcgcaa tgaaatatgc
     8941 ggatgccttg atctcgggca gcgaaaaact ggataaagag gttataactg caaaaaaatc
     9001 gcttggcaaa aaaccacaac tggactatct ccctgaggaa ggacgtaacc aggcctatct
     9061 tcacttctac gaaaccctgc tcaagtaatt acttccaggg ccaagaagca ttcagaatga
     9121 gaaattcaat aatttccctg ttcaccctgt ttttaagcct gatttttatc aggtgcaacg
     9181 acccggccat cctcgg
//
LOCUS       DAOQXT010000149         9133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_8097_length_9133_cov_5.890394, whole genome shotgun sequence.
ACCESSION   DAOQXT010000149 DAOQXT010000000
VERSION     DAOQXT010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_9133_cov_5.890394"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..548
                     /locus_tag="PKM27_17835"
     CDS             75..548
                     /locus_tag="PKM27_17835"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNR09187.1"
                     /translation="MKRIKKILCPVDFSDMSEETLEIAIQLAEKYAAAFHIIYVLPRP
                     NFYDWTLTGMSNIVLDDWFEKTKKDVDKKIKAMVEMIRADHPFVEVTSEISDKMDPAE
                     GILESAKNTKAELIIMGSHGRKGLNRILMGSVAESVLRHAPCGVMIYKSKPTKKK"
     gene            681..839
                     /gene="ccoS"
                     /locus_tag="PKM27_17840"
     CDS             681..839
                     /gene="ccoS"
                     /locus_tag="PKM27_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNR09188.1"
                     /translation="MSVIIILLIASLSVALVFLLAYLWSVRDGQFDDVFSPGERILFE
                     DKKPIDKK"
     gene            854..2950
                     /gene="ccoN"
                     /locus_tag="PKM27_17845"
     CDS             854..2950
                     /gene="ccoN"
                     /locus_tag="PKM27_17845"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNR09189.1"
                     /translation="MDKFYYDNKIVKQFGVATLFWGIIGMLVGVLIAFQLAFPALNFG
                     IPYTSFGRLRPLHTNAVIFAFVGNAVMGGAYFILQRVLKTRLFSDLLSKIHFWGWQLI
                     IVFGAITLVAGYTTGKEYAELEWPLDILITLIWVVFGINMVGTILKRRERHLYVSVWF
                     FIGTWVTVAMLHIVNSFEIPFTWLKSYSLYAGVQDALVQWWYGHNAVAFFLTTPFLGL
                     MYYFVPKAAERPVYSYRWSIIHFWALIFIYIWAGPHHLLYTALPEWAQALGTVFSIML
                     ILPSWGGMLNGLFTLRGAWDRVREDPILKMLVVSLTCYGMATVEGPMLSLKNVNAYSH
                     YTDWTVAHVHIGALGFNGFMIFAMLYYLVPRMWNTTLHSRQLANVHFLISTIGILLYA
                     IPMYWAGWAQSLMWKKFTDSGQLQYQFLETVTHIVPMYIVRGIGGTLYWIGAFLAVYN
                     LYKTMKKGSFVDNEVAEALPLSAQPIPVKQHWHNAIERKPVQMLALSFVVVAIGGIVE
                     LVPTFLVESNVPTISSVKPYTPLELHGRDLYVREGCYTCHSQMIRPFRDEEMRYGEYS
                     KSGEFVYDYPFQWGSKRTGPDLHRIGGKYPDSWHYNHMRDPQETSPGSVMPPYPWLLE
                     KELNTRTTPAKIRAMQKLGVPYPEGYDKISNEELRKQAERIAAGLKGSKIEVSADKEI
                     IAMIAYLQRLGTDIKK"
     gene            2965..3123
                     /locus_tag="PKM27_17850"
     CDS             2965..3123
                     /locus_tag="PKM27_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09190.1"
                     /translation="MKFSNYLKSIEDVSIYPLITLVLFVLVFVSVVVYTFMISKQDMD
                     ERANIPLK"
     gene            3135..4277
                     /locus_tag="PKM27_17855"
     CDS             3135..4277
                     /locus_tag="PKM27_17855"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNR09191.1"
                     /translation="MKCLILKDSLVRSILTASVFITGIPGMAGLAAQTNGVAGPQAVS
                     YDAFVITRWILLGLSAILLVILLAMARSVLAVARLKIQNFTGNPNSPARSLAKPLSLL
                     GLMMISTSVSGQSGLAGAANSVPLDIYLLIAAILLELLIMLFLVRTLFQFVGIQKMKP
                     AVERVKKPSLFQRLNQTVAIDREAELDLNHNYDGIRELDNKTPAWWTYAFYISILFGA
                     IYLYRMFVSKSLPGQLIELAQENERAEILQSQYLKTAASAIDENNVTMLDPESVAKGA
                     MTFKVNCAVCHGVNGEGNVVGPNLTDDYWIHKGSIKDIFYTIKYGVPEKGMKSWKDDF
                     SPVQIAELASYVKSLHGTNPANAKEKQGELFVEESPAAAPDTTGGK"
     gene            4313..4762
                     /locus_tag="PKM27_17860"
     CDS             4313..4762
                     /locus_tag="PKM27_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09192.1"
                     /translation="MQDKKKILQQIRNHQEAMISELKATIQNLEHEADLPEGEVIDPE
                     DASFQSASMEMLSHYRTLLVNAENDLYKLNNLSEVETDRVAPGALVVTDEILLYIGIP
                     VKSIPMSGGRTLIGVSPDSEIVNQLEAKKTGDAFDFGDTTLVIRSIS"
     gene            4785..6173
                     /gene="ccoG"
                     /locus_tag="PKM27_17865"
     CDS             4785..6173
                     /gene="ccoG"
                     /locus_tag="PKM27_17865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNR09193.1"
                     /translation="MSKETTDFKDKITTADKAGKREWIYALQPKGKLYTWRTWLSYVY
                     LVVLVALPFIKINGESLFLLNVVEGKFIFFGKIFLPQDFILFGLAMLTLLLFIIVFTL
                     IFGRIFCGWICPQTIFMELVFRKIEYWIEGPANKQKILNEQPWTTDWYMRKGLKHLIF
                     FFVSFLISNVFLAYIIGIDALISIVSEPVTRHLVGFIAILAFSFVFYLVFAFVRELVC
                     TVICPYGRLQSVLLDKNSIVVAYDYVRGEPRARVSRTRSEEAGDCIDCDLCVKVCPTG
                     IDIRNGTQMECTNCTACIDACNLMMRNTGRPENLILYASENNIRNGEKPRLTYRVKAY
                     SAVLIALVGLMGFLLAERSMFDTTVLRVPGQVLQENADGTISNLYRMKIINKSSLTRD
                     YHLKLKNTDAELVYAGRQVDSLHSGQGVEELFFIKKPKSSITERKEILLMDVLSGNKK
                     VQTKKITFIGRY"
     gene            6179..6619
                     /locus_tag="PKM27_17870"
     CDS             6179..6619
                     /locus_tag="PKM27_17870"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNR09194.1"
                     /translation="MNKWFKSGNLILLGGGIFLLFIFSLIFGFMAQDLQLVTPDYYEQ
                     ELLYNQRKKAIRNAESIDSLLVLKKLEGVIELSVPPRLALQLKSGSAHFYCPADAKAD
                     RVMPLQANGEGKFILSTSGLKNCPYRLKVSLDDGDQKYFKEFYL"
     gene            6629..7336
                     /locus_tag="PKM27_17875"
     CDS             6629..7336
                     /locus_tag="PKM27_17875"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNR09195.1"
                     /translation="MSTTLVVSAFILGVGGSLHCLGMCGPLVMALPFGSTRTEHTALN
                     LSLYLVSKALMYGVLGLAVGAAGLGFKWITGQLILSVAAGIFVLAVTLWPWISRKMTL
                     SFDLSDKIRSRFQKLLSHPAWYSFVLYGIFNALLPCVMILVAFGASAATGHPLSGFAF
                     MFVFGLGTVPALLAAYFSGTMITRRFRLNLQWTSRVIAILLGVVLVIRGLNIHIPHSS
                     LPLINHVSKIISCPAPE"
     gene            7337..>9133
                     /locus_tag="PKM27_17880"
     CDS             7337..>9133
                     /locus_tag="PKM27_17880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNR09196.1"
                     /translation="MKPTLASLTCFHCGDRCPDDRIVYGDKHFCCAGCRTVYGILHEN
                     DLDQYYCLNETPGINARKTAPTLFSFLDDPEWASRFITFSSPSLVKAQFYLPQIHCSS
                     CLWLLEHLHKLHPGILDSRVNFMEKRVYLSFDPEKINLRGVAEQLTSLGYEPLIELNR
                     PGPPSSALDKTAIQIGISGFCFANIMLLSFPEYFGLNLRQDGSLVHFFRYLNLGLGIP
                     VFLIGLDVFFRNAWTGLKQKYLNIDGPVSLAILVTFIRSLVEILSGSGMGYLDSMSGI
                     VFFMLVGRAFQNRTNKSLQFSRDYESYFPISVQVKEGDQVSSRNIRDVKKGDILLLKN
                     GEILPVDGMLSIGHSLIDYSFITGESQPVAVSPGEWVYSGGRVEGSPVEILTVKPFSQ
                     SGFTQLWNNEVFRKEKNTGHYKTDLLAKYFTILVLGIAGTAFTYWQLREPSVSWNAFT
                     AVLIVACPCALLLSSSFTYGHLIRLFSNLQFFVKNAAVIETLPLINHLVFDKTGTLTH
                     SNAGTVSLRFMDWTPEEESMALSLMAGSSHPLSRSILSWARPAVDCPVASYREIPGGG
                     IEGWVMDRHIRIGNEKFTGHEGAGGPGSYPRPS"
BASE COUNT         2177 a         2360 c         2292 g         2304 t
ORIGIN      
        1 ttgtggtcat gcgggcacat attgataaaa atcaaatttg taatattgtt ctgctttaac
       61 aaaaaattaa aagtatgaaa cgcattaaaa aaatcctgtg tcccgtggat ttttccgata
      121 tgtcggagga aaccctggag atcgccatcc agcttgctga aaagtacgcg gctgctttcc
      181 atattattta tgtcttgccc cgccctaact tttatgattg gaccctgacc ggcatgtcca
      241 atattgtgct ggacgactgg tttgagaaaa ccaaaaagga tgtggataaa aaaatcaagg
      301 cgatggtcga gatgatccgg gccgaccatc cgtttgtcga agtgacctcg gaaatcagtg
      361 ataaaatgga cccggctgaa ggcatcctcg agtcggccaa aaataccaaa gcggaactga
      421 tcatcatggg ctcgcatgga aggaaagggc tgaaccggat tctcatgggc agcgtggccg
      481 aatcggtact acgccatgcc ccttgcggcg tcatgatcta caaaagcaaa ccaacaaaga
      541 aaaaataaga aatcgggctg tgaatatcct tttcctggta ggtctatagt aagaatataa
      601 agcaacacca aagggaagtt acttttaagc tatagtcggg tatcacagcc ttttttatgt
      661 aaccttaaaa agtccatccg gtgtccgtca tcatcatcct gctcatcgca agcctgtccg
      721 tagccctggt atttcttctc gcttatttgt ggagcgtcag ggacggccag tttgatgacg
      781 tattttcgcc gggtgaacgg atcctttttg aagacaaaaa acccattgat aaaaaataaa
      841 ttttgtgagt actgtggata agttttacta cgacaataaa attgtcaaac agtttggagt
      901 agccaccctt ttctggggca ttatcggaat gctggtgggc gttttgatcg ctttccagct
      961 cgcttttccg gcccttaatt tcggcatccc ttataccagt ttcggaaggc tgaggcccct
     1021 gcataccaat gccgtcatct tcgcctttgt gggcaatgcg gtcatggggg gggcatattt
     1081 tatcctccag cgggtattaa aaacccgcct gttcagcgat ctgctcagca agatccattt
     1141 ctggggctgg cagctgatca tcgtatttgg agccattacc ctggttgcgg gatataccac
     1201 cgggaaggag tatgccgaac tggagtggcc gctggatatc ctgatcaccc tgatctgggt
     1261 ggtttttggc attaacatgg tcggcaccat ccttaaaagg agggaaaggc atctatacgt
     1321 ctccgtctgg ttttttattg gtacctgggt cacggtggcc atgctgcata tcgtcaactc
     1381 gtttgagatt cccttcacct ggctcaagag ttacagcctc tatgccggag tccaggatgc
     1441 cctggttcaa tggtggtacg gacacaatgc agtggccttt ttcctgacca ctcctttcct
     1501 gggactcatg tattattttg tgcccaaagc ggccgaacgc ccggtatatt cttatcgctg
     1561 gagcatcatc catttttggg cgctgatctt catttacatc tgggccggcc cccatcacct
     1621 cttatatacc gcgctgccgg aatgggcgca ggccttgggt acggttttca gcatcatgct
     1681 gatcctgccc agctggggag ggatgctcaa cggcctgttc accctccgag gcgcctggga
     1741 ccgggtccgg gaagacccca ttttaaagat gctggtggtc tcgctgacct gttatgggat
     1801 ggccactgtg gaagggccga tgctttcact caaaaatgtc aatgcctaca gtcattatac
     1861 tgactggacc gtggcccacg tacatattgg agcactgggc ttcaacggat ttatgatttt
     1921 tgccatgctt tattacctgg tgccccgcat gtggaacacc accctgcact ccaggcaatt
     1981 ggccaatgtg cacttcctca tctccaccat aggtatcctg ctctatgcca tccccatgta
     2041 ttgggcaggc tgggcccagt ccctgatgtg gaaaaaattt acggatagcg gccagttgca
     2101 ataccagttc ctggagacgg tgacccatat cgtcccgatg tatatcgtgc gcggcatcgg
     2161 agggacttta tattggatcg gcgccttcct ggctgtgtac aatctgtaca aaaccatgaa
     2221 aaaaggaagt tttgtggaca atgaagtggc tgaggccctt ccgttgtccg cccagcctat
     2281 tcctgtcaaa caacactggc acaacgccat cgaaaggaaa cccgtgcaaa tgctcgccct
     2341 cagttttgtg gttgtggcca tcggcggtat cgtcgaactg gtgcccacct tccttgtgga
     2401 aagcaatgtg ccgaccatct cttcggtcaa accttatacc ccgctcgaat tgcacggccg
     2461 cgacctctat gtgcgggaag ggtgttatac ctgccattcc cagatgatcc gtcctttccg
     2521 ggatgaagaa atgcgttatg gcgagtacag caaatccggt gaatttgtgt acgattatcc
     2581 cttccagtgg ggcagcaagc gcaccggacc ggacttgcac cggattggcg ggaaatatcc
     2641 ggacagctgg cattacaacc acatgcggga tccgcaggag accagcccgg gatccgtgat
     2701 gcccccttat ccctggttgc tggagaaaga gctcaatacc cgcaccacac cagcgaaaat
     2761 aagggccatg caaaagctgg gggtccccta tccggaggga tatgataaaa tcagcaatga
     2821 agaactgcgt aaacaggccg aacggatcgc tgccggcctg aaaggaagca agatagaggt
     2881 gagtgcggat aaggagatca tcgccatgat cgcttacctg caacgcctgg gaaccgatat
     2941 taaaaaatga attaagtccg aatcatgaaa ttttccaatt atctcaaatc catagaggat
     3001 gtcagcatct atcccctgat cacccttgtt ctatttgtcc tggtctttgt gtccgtggtc
     3061 gtgtatacct tcatgatcag caaacaagac atggatgaac gcgccaatat tcccttgaaa
     3121 tgaaaatcag atgtatgaaa tgcttaatct tgaaggatag cctcgtccgc tccattttga
     3181 cggcttccgt ttttataacc ggcatacctg gtatggcggg actcgccgct caaaccaacg
     3241 gggtggccgg acctcaggcc gtctcttacg acgcttttgt gatcacccgg tggatcctgc
     3301 tcggcttgtc ggccatcctg ctggtgatcc ttttggctat ggcccgttcc gtcctcgccg
     3361 tcgcccgtct taaaatccaa aattttaccg ggaatccgaa tagccctgcc cgaagcctgg
     3421 ccaaaccgct tagcctgctt gggctgatga tgatatccac ttccgtttcc gggcaatcag
     3481 gtctggcggg cgcggcaaac tcggtgcccc tcgacattta cctgctgatc gccgccattt
     3541 tgctggaatt gctgatcatg ttattcctgg tgaggaccct cttccagttt gtcgggattc
     3601 aaaaaatgaa acccgccgtt gaaagagtga aaaaaccctc gcttttccaa aggctcaacc
     3661 aaaccgtagc gatcgaccgg gaagcggaac tggacctaaa ccacaactac gacggcatcc
     3721 gcgaactgga caacaagacc cctgcctggt ggacctatgc attttatatt tccatccttt
     3781 tcggagccat ttacctgtac cgcatgtttg tcagcaagtc ccttcccggt cagctgatcg
     3841 aactggccca ggaaaatgag cgggccgaga tcctgcaatc ccagtatttg aagactgcgg
     3901 ccagcgccat tgatgaaaat aatgtcacga tgctggatcc ggaaagcgtg gctaaagggg
     3961 ccatgacgtt taaggtcaat tgtgccgtct gtcatggggt gaatggggaa ggcaacgtgg
     4021 ttggccctaa cctgacggac gattactgga tccacaaagg atccatcaag gatatctttt
     4081 acaccatcaa atacggcgtt ccggagaaag ggatgaagag ctggaaggat gatttctcgc
     4141 cggttcagat cgcggaactg gcttcttacg tcaaatccct gcatgggacc aatccggcca
     4201 atgccaaaga aaaacagggc gaactgtttg ttgaggaaag cccggcggct gccccggata
     4261 ccaccggggg caaataacag gattcgtaca tgcgtttaaa tcaataacga ctatgcaaga
     4321 taaaaagaag atcctccagc agatccggaa tcatcaggaa gccatgatca gcgaattgaa
     4381 agccacgatc caaaacctcg agcacgaggc agatttaccg gaaggggaag tcatagatcc
     4441 tgaagatgct tccttccaga gcgcatccat ggagatgttg tcgcattacc ggaccttgct
     4501 ggtgaatgcg gaaaacgatt tatacaaact gaacaacttg tccgaggtgg aaaccgaccg
     4561 agtggcgccg ggagccctgg tggttaccga tgaaatcctg ctttacatcg gaataccggt
     4621 gaaatccatc ccgatgtccg gaggcaggac cctgatcggg gtttccccgg actccgaaat
     4681 cgtaaaccag cttgaagcca aaaagaccgg agatgccttc gattttgggg acactaccct
     4741 ggtcatccga agcattagct gacctttttt aaacacatca agttttgtca aaggaaacca
     4801 cggattttaa agacaagatc accacagcag acaaggccgg caagagggag tggatttatg
     4861 cgctccagcc aaagggcaaa ctttatacct ggcggacctg gctcagttat gtttacctgg
     4921 tggtcctcgt ggctcttcct tttataaaaa taaacggaga gtcgcttttc ctgctcaatg
     4981 tggtggaggg caaattcatc tttttcggaa agatctttct tccccaggat tttatccttt
     5041 ttggcctggc catgctcacc ctgctgctgt tcattattgt attcaccctg atcttcggcc
     5101 ggattttttg cggctggatc tgcccccaga ccatttttat ggagctggtt ttccgtaaaa
     5161 tcgagtattg gatcgagggt cctgccaaca aacaaaaaat cttgaacgaa cagccctgga
     5221 ctaccgactg gtatatgcgg aaaggcctga aacacctgat cttctttttc gtttccttcc
     5281 tgatttccaa tgtattcctc gcttatatca ttggcatcga tgcgctgatc agtattgtca
     5341 gcgagccggt gacgcggcac ctggtcggat ttattgccat cctcgccttc tcttttgttt
     5401 tctacctcgt atttgctttt gtccgcgaac tggtatgcac cgtcatttgt ccttacggca
     5461 ggcttcaaag tgttttgctg gataagaatt cgattgtggt ggcctatgat tatgtcaggg
     5521 gagaaccccg ggccagggtc agccggaccc ggtcggaaga ggccggggac tgcatcgatt
     5581 gtgacctctg tgtcaaggtt tgcccgacgg gtattgatat ccgcaacggc acccagatgg
     5641 aatgtaccaa ttgcaccgcc tgtatcgatg cctgcaacct gatgatgcgg aacaccggcc
     5701 gtccggaaaa cctgatcctc tatgccagcg aaaacaatat ccgcaatggg gaaaaaccca
     5761 gactgaccta ccgggtcaaa gcatacagcg ccgtcctgat cgccctggtg ggcctcatgg
     5821 gcttcctgct ggcggaacgt tccatgtttg acacgacggt attgagggtt cccggccagg
     5881 tattgcagga aaatgcggac gggaccatca gcaatcttta ccggatgaaa atcatcaata
     5941 aatccagtct gacgcgggat tatcacctga aactgaaaaa taccgatgcc gaactggtct
     6001 atgccggccg gcaggtggat tccctgcaca gcggccaggg ggtggaagaa ttgtttttta
     6061 tcaaaaaacc gaaatcgtcc atcacggagc ggaaagagat cctcctcatg gacgttttat
     6121 caggaaataa aaaagtacaa actaaaaaaa tcaccttcat cggaagatat tgaccactat
     6181 gaataaatgg tttaaaagcg gcaatttgat cctcctggga ggcgggattt tcctcctgtt
     6241 tattttcagc ctgatctttg gattcatggc ccaggacctc cagctggtca ctcccgacta
     6301 ttatgaacag gaacttcttt ataaccaaag aaaaaaagcg atccggaacg cggagagtat
     6361 agacagcctc ctggtcctca aaaaattgga aggggttatt gaattgtcgg taccaccccg
     6421 ccttgccctt caactgaaat cgggttcagc tcatttttat tgccctgccg atgcaaaggc
     6481 tgaccgggtc atgccccttc aggccaacgg ggagggcaaa ttcatcctga gtaccagtgg
     6541 attgaaaaac tgtccttacc gtctgaaagt ttcgctagac gacggggatc aaaaatattt
     6601 taaagagttt tacttataag caaggcccat gagtacaacc ctggtcgtat cggcatttat
     6661 cctcggtgtg ggcggaagcc tccattgcct gggtatgtgc ggtcccctgg tgatggccct
     6721 gcctttcggc tccacccgaa cggaacacac cgctttaaac ctcagcctgt acctggtttc
     6781 caaagcactg atgtatggag tccttggact ggccgtcggc gctgccggct taggtttcaa
     6841 atggattacc ggccagctta tattgtctgt cgctgccgga atctttgtcc tggcagtgac
     6901 cctgtggccc tggatcagca gaaaaatgac cctgtctttt gacctaagcg ataaaatcag
     6961 atccaggttt cagaaattat tgtcccatcc ggcctggtat tcatttgtat tgtacgggat
     7021 attcaatgcc ttgctgccct gcgtcatgat cctcgttgct tttggggctt cagcggccac
     7081 cggccatccg ctcagcggtt ttgcgtttat gtttgtgttc ggcctgggga cggtgcctgc
     7141 ccttctggcg gcttattttt ccggtacgat gattacgagg aggtttcgac tgaatctgca
     7201 gtggacatcc agggtcatcg ccatcctcct gggagtggtc ctggtcatcc ggggcttgaa
     7261 cattcatatt cctcattcca gcttgccgct gatcaatcat gtttcaaaaa ttataagttg
     7321 cccggcgcct gaataaatga aaccaaccct cgccagtctg acctgtttcc attgcggcga
     7381 tcgctgcccg gatgaccgta ttgtatacgg ggacaagcat ttttgttgcg ccggatgccg
     7441 cacggtctac ggcatacttc atgaaaacga cctggatcag tattattgcc tgaacgagac
     7501 tcccgggatc aatgcccgca aaacggcgcc caccttgttt tcgttcctgg atgatcccga
     7561 atgggcctcc cggtttataa ccttctccag ccccagcctg gtcaaggctc aattttacct
     7621 gccccagatc cattgcagtt cctgcctctg gctgctggaa caccttcata aactccaccc
     7681 gggcattcta gactcccggg taaactttat ggagaaaaga gtctacctgt cctttgatcc
     7741 ggaaaaaatc aatctccggg gcgtcgccga gcaacttact tccctgggtt atgaaccgct
     7801 cattgaactc aatcggcctg gccccccgtc atccgcactg gataaaacag ccattcagat
     7861 cgggatctcc ggcttttgtt tcgccaatat catgctcctg agtttcccgg aatactttgg
     7921 gctaaacctc cggcaggacg gatccctggt ccattttttc cgctatctca acctgggcct
     7981 gggcataccg gtattcctca ttggcctgga tgtctttttt cgcaatgcct ggaccggcct
     8041 gaaacaaaag tacctcaata ttgacggccc ggtatccctg gctatcctcg tgacttttat
     8101 aagaagcttg gttgaaatct tgagcggttc cggcatgggg tacctggaca gtatgagcgg
     8161 cattgtattt tttatgttgg tcgggagggc ctttcagaac agaaccaaca aatcccttca
     8221 gttcagccgg gattacgaat cctattttcc gatttccgtc caggtgaaag agggggacca
     8281 ggtaagttcc cggaatatac gggacgtaaa aaagggggat atccttttat taaaaaatgg
     8341 agagatcctt cctgtcgacg ggatgctcag cattggccac agcctgatcg attacagttt
     8401 tataaccggc gagtcccagc cggtggcggt aagtcccggg gagtgggtct attcaggggg
     8461 cagggtggag ggcagccccg ttgaaatcct taccgttaaa cctttttcgc aaagcggatt
     8521 tacccagttg tggaacaatg aggtattccg gaaagaaaaa aatacaggcc attacaagac
     8581 agacttgctg gcgaaatatt ttaccatcct cgtgctgggc attgccggga ccgcctttac
     8641 ttattggcag cttcgggagc cctcggtttc ctggaacgcc ttcacggccg tattgatcgt
     8701 agcctgcccc tgcgccctgc tgctgagttc atcctttacc tacggccacc tcattcggct
     8761 cttttcaaat ctgcagttct tcgtcaaaaa tgcagcggtg atcgaaacgc tgcctttgat
     8821 caatcacctg gtctttgata aaacgggcac cctgacccac tccaatgcgg gcactgtatc
     8881 gctgcgattc atggactgga caccggaaga ggaatccatg gcgctcagcc tgatggccgg
     8941 ctccagccat ccgctcagcc ggtctatcct gagttgggcc aggccggcgg tcgattgccc
     9001 ggtagcctcg taccgtgaaa ttcccggggg cggcattgaa ggctgggtga tggacaggca
     9061 tatccggatc ggcaatgaaa aattcaccgg acacgaaggc gccggggggc caggaagtta
     9121 tccacgaccg agt
//
LOCUS       DAOQXT010000150         8893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_8337_length_8893_cov_5.758090, whole genome shotgun sequence.
ACCESSION   DAOQXT010000150 DAOQXT010000000
VERSION     DAOQXT010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8337_length_8893_cov_5.758090"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1858
                     /locus_tag="PKM27_17885"
     CDS             155..1858
                     /locus_tag="PKM27_17885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR09197.1"
                     /translation="MKTSLLFIAGFLALSTFSRAQITQTTEEAVQTESIFIEALRNKM
                     IGRYDLAIGQYLEILKKQPDNHVVHYELAAAYLNTKKPNEALEAIKNAVKGAPDQIFY
                     LQLQADVYHTLQDESAEASVYKKLQSLAPSKELYYDKWSSLLMNLGQDAEALRVLDLM
                     EREKGINEIGSFRKTQILERQGKGKEAEAELVKLNEAFPKDLKYLHALAGFYKRTKRD
                     SKALEIYKMILVLAPQDEAANLALASTYKTAGQDSKYLEAISGLMSNPSISIDAKVLE
                     MIPYLQKAISQKDQALLEVLEKHAATLVASHPGEAKPFAFYADILLEKGRYRAAYEHY
                     RSALALEDRVAPVWEQFLSLSARFDPPASHLEMCEKAFDLFPNHPGLAFQYAHAFYQN
                     EQYPECLNLLNQAMLMAGEDKLLLEKIWCLKGVAELKSGRQNEAEASFASALQLNPSS
                     LAAKQMQAYALASVPESLDKAQELIQQAIRQSPDQYELEYTQAYILVKQKKTADARKW
                     FEASLNHGGSGHVVILEQYGDLLYQLKEFEQAVQTWQKALELSPDNIRLKKKIADRSA
                     E"
     gene            1855..2604
                     /locus_tag="PKM27_17890"
     CDS             1855..2604
                     /locus_tag="PKM27_17890"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNR09198.1"
                     /translation="MKIKPGLYLLLLIFGSASCSRKPLMSSGDKPDVRSVQSVISAVN
                     TKGIQSRWVQARAQVSLNGGGFLTSGTASIRSCKDSLIWISVSKLGFEVARGIILADS
                     AFWINSLENTYWKGSTAELNKKYRVPARLGDLQSFIFPAIDPTCEYALENKDQAWWLS
                     QPGQLQRRYEISPLSHLIQSISLSHEGTDLKFRYGDYKATENFWFPYEQTLYIRQNED
                     LHETRLKFNQVQAAQYLQTPFHIPSGFTPVD"
     gene            2643..3854
                     /gene="hflX"
                     /locus_tag="PKM27_17895"
     CDS             2643..3854
                     /gene="hflX"
                     /locus_tag="PKM27_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNR09199.1"
                     /translation="MKDQFNLNNKAAAYRQGYQEKAILVGVATGQQSESQVTEFLDEL
                     EFLASTAGAVVVKRFFQRLPHPDSRTFVGKGKLEEISQYVESNAIDLVIFDDDLSGKQ
                     TNTIEEALKVKIVDRSSLILDIFAKRAQTAQAKTQVELAQMQYLLPRLRGLWTHLERQ
                     RGGIGMRGPGEQEVETDRRLVKNKISILKEKLEKIDQQAFTQRRSRGELIRVALVGYT
                     NVGKSTLMNLLSKSDLLAENKLFATLDTTVRKVYFEDMPFLLSDTVGFIRKLPHHLVE
                     SFKSTLDEVRESDLILHVVDLSHPQYEDQIGTVNKTLEELGVSSKSILMVFNKMDAYR
                     EKYFDRLLSPEEKKELEDQLVQMLKSRYPVPMVVISAAKKENMEEFRRILTRMVKEQY
                     VVRYPYMVKSW"
     gene            3857..4966
                     /locus_tag="PKM27_17900"
     CDS             3857..4966
                     /locus_tag="PKM27_17900"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNR09200.1"
                     /translation="MHQKQLEAYSRLLLDYSLRLGRGDKLYIQTTTLALPLVRQLYEG
                     ALRRLAVPVIHMSFEGQEELQNQLAHEEFIRYINPAYAEAVEQFHAFLFIRAPFDDRG
                     FPPMDKGLQALRREVTGPLQKTYAIRTGNQSLRRTLCQYPTPAGAKEASMTTEEFGDF
                     IAAACFLDETRPEESWKQLGREQQAYVDFLNGVKQITFRNEKTEMTCSVDGRTWINSD
                     GKNNMPSGEVYTSPVEDSVEGRIYFDYPFTYEHHTIRGVSLRVEKGKIVEAGAEEGNE
                     KLQEILEIPGARSFGEIAIGTNTHIQRPTCNILFDEKMAGTVHMAIGQSYLQCGGKNE
                     SAVHQDMIADMRRGGQIWADGRLIYENGVFLEVGS"
     gene            5393..6091
                     /locus_tag="PKM27_17905"
     CDS             5393..6091
                     /locus_tag="PKM27_17905"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNR09201.1"
                     /translation="MKNKLNLLLLSSILALGACERDLGEYVIIPVNTGPFSQLRQDAS
                     TDIVLRQGPGLLVEFEGYEDIFYDLDFRVSGGKLIISQRGHSWSSGRSKIYITVPDLT
                     KIEITASGDVYGDNRFRINGDIELHSRASGDFDLNLDVDEVYTWIEGSGQVRLRGYAD
                     LLEAEIDGSGDLYAFPLNSRNTRITSYGSGDAEIYATHNLYVRLRGSGDIYFTGYPYV
                     DYLVTGSGRLIDAN"
     gene            6354..8357
                     /gene="rpsA"
                     /locus_tag="PKM27_17910"
     CDS             6354..8357
                     /gene="rpsA"
                     /locus_tag="PKM27_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNR09202.1"
                     /translation="MLEEKDEIQDMEKNIPPAKEETEDQTGSAEPEMAEEDGALIEVP
                     ELELELEAVQEENTAHDDFNWSAGNRNTRYYSDEEKKSYLNKYTTTLSTLGDHEIIKG
                     KVSSIHGSDVVLDINYKSDGLIPYDEFKDMGDLKVGDFVDVYVESQENAKGQLVLSRK
                     KAKLLKAWESLVDSFNNGTIIKGTVVSKTKGGLIVDASGLETFLPGSQIDIKPITDYD
                     QYVGKTMEFKVVKINETIKNAVISHKALIESDLAEQRDAIISGLEKGQVLEGVVKNIT
                     DFGAFMDLGGVDGLLYITDISWGRVNHPNEVLAMNQKLNVVVLDFDENKKRISLGLKQ
                     LQPHPWDVLGDTIKEGDVVKGKIVNIEDYGAFLEISPGIEGLIHVSEVSWSNQPVNSR
                     DYFQLGQEFEAKVVTIDKEERKMSLSLKQLSQDPWTTISEAYPVGSRHTGVVKNLTPY
                     GVFVELGEGIGGMIHISDLSWTKRFSHPSEFISIGEKIDIMVLEIDKDNRKLSLGHKQ
                     VEENPWDTFEQVFPVGSYHEATVIRKDDKGGAIVQLPYGLEAYAPQKHIKKENGSYAE
                     VDNVLTFKVIEFNRDDKRILVSHTRFLDDIRKEASKAVKGSGDQATDEAEKKAALKAA
                     KALTTKAEKTTLGELAEFSALQQKLKGNISPEPTDKSEEEKEK"
     gene            8527..>8893
                     /locus_tag="PKM27_17915"
     rRNA            8527..>8893
                     /locus_tag="PKM27_17915"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2453 a         2201 c         2300 g         1939 t
ORIGIN      
        1 caatagtcaa cagttagtag tcagtaatca gtagcagcaa tcagaagtca gcttcagcaa
       61 ttagggctga tggttcgaag ttgattttta tgaatccctt aaataaatac ctccttcggc
      121 cattgccagg ctccttacct ttgcacggga ttgtatgaaa accagtctgt tattcatagc
      181 agggttcctt gcgctcagca ccttcagccg tgcccagatc acccaaacca ctgaagaggc
      241 cgttcagacc gaatccattt ttattgaagc cctgcgcaat aaaatgattg gccggtatga
      301 cctggccatt ggccaatacc tcgaaatatt gaaaaaacaa ccggacaacc atgtggttca
      361 ttacgaactg gcggccgctt atctgaatac caaaaaacca aacgaagccc ttgaggccat
      421 aaagaacgcc gtcaaagggg ccccggacca gatcttttat ctccagcttc aggccgatgt
      481 ttaccacacc cttcaggatg aatcggcgga agcttcggtc tataaaaaat tgcagtctct
      541 cgccccttca aaagaattat actacgacaa atggtcttcc ctgttgatga acctgggaca
      601 agacgcggag gccctcaggg tccttgacct gatggagcgg gaaaagggga tcaatgagat
      661 cggctctttc cggaaaaccc agatcctcga aaggcaggga aaagggaaag aagccgaagc
      721 ggaactggta aaactgaacg aagcctttcc gaaggacctc aaatacctgc acgccctggc
      781 aggtttttat aaaagaacca aaagggattc caaagcgcta gagatctata aaatgatcct
      841 ggtcctcgca ccccaggatg aagccgccaa cctggccctc gcctcgacct ataaaacagc
      901 cggccaggat tcgaaatacc tcgaagccat aagcggcctg atgtccaatc cgtccatttc
      961 cattgatgct aaagtgctgg agatgatccc ttatctccag aaagcaatca gccagaagga
     1021 ccaggcgctg cttgaggtcc tcgaaaaaca cgcagcgacc ctggtggcca gtcatcccgg
     1081 cgaggcgaaa ccttttgcct tttacgccga tatccttttg gaaaaaggga ggtatcgtgc
     1141 agcttatgaa cattaccggt ccgccctggc cctggaagac cgggtggcgc ccgtctggga
     1201 acaattcctc agtctttccg cacgatttga tcccccggcc agccatctcg aaatgtgcga
     1261 aaaagccttc gacttgtttc caaatcatcc cggcctggcc ttccagtacg cccacgcttt
     1321 ttaccagaac gagcaatacc cggaatgcct gaatctgctc aaccaggcca tgctgatggc
     1381 cggggaagac aaactcctgc tggaaaaaat ctggtgcctc aagggtgtgg cggagctgaa
     1441 atcaggccgg caaaacgaag cagaagcttc gtttgcaagc gcgcttcagc tgaacccatc
     1501 ctccctggcg gcaaaacaaa tgcaggctta cgccctggcc tcagtacccg aaagcctgga
     1561 taaagcccag gaattgatcc agcaggcaat ccggcaatcc cccgaccagt atgaactgga
     1621 atatacccag gcctatattt tggtaaaaca gaaaaaaacg gccgatgccc ggaaatggtt
     1681 tgaagcctcc cttaatcacg gaggatccgg ccatgtcgtc atccttgaac aatacgggga
     1741 cttgctttat cagttgaaag aatttgagca ggcggtccag acctggcaga aggcgctgga
     1801 actcagtcca gacaatatcc gtttgaaaaa gaaaatcgcc gatcgttccg cagaatgaaa
     1861 atcaaacccg gtttatatct tctcttactg atcttcggtt cagcatcctg cagccgcaag
     1921 cctttgatga gttccgggga caagccggac gtgcgctccg ttcaaagcgt gatcagcgcg
     1981 gtcaatacca aaggaatcca atcccggtgg gtgcaggcca gggcgcaggt ttcgctgaat
     2041 ggcggcggat ttttaaccag cgggacggct tccatccggt cctgcaagga ttctttaata
     2101 tggatcagcg tatccaagct gggatttgaa gtggccaggg gcatcatcct ggccgattcc
     2161 gctttctgga tcaacagcct ggaaaatacc tattggaagg gcagtactgc cgaactaaat
     2221 aaaaaataca gggtgccggc ccgattgggg gatcttcaat cgtttatttt ccctgccatc
     2281 gatccgacct gtgaatatgc actggaaaat aaggaccagg cctggtggct cagccaaccg
     2341 ggccagttgc aacggcgtta tgaaatttca ccgctttctc acctgattca aagtatttcc
     2401 ctgagccatg agggcacgga cctgaaattc cgttacggcg attacaaagc cacggaaaat
     2461 ttctggtttc cttatgaaca aacgctgtat atccgccaga atgaggacct gcatgaaacc
     2521 aggctgaaat tcaaccaggt gcaggcggct cagtatcttc aaaccccctt ccatatccct
     2581 tccggtttta ccccggtgga ttagaaggcc tccgcaagga ccacccacga atattgaaaa
     2641 ccatgaaaga ccagtttaat ctcaataata aggccgctgc ataccggcag ggataccagg
     2701 aaaaggcgat cctggtaggt gtggccaccg gtcagcagtc cgaaagccag gtcaccgaat
     2761 ttctggatga gttggaattc ctggcatcca ctgccggagc cgttgtggtc aaacgctttt
     2821 ttcagcgcct gccccatccg gattcaagga cctttgtcgg caagggaaaa ctggaagaaa
     2881 tcagccagta tgtagagtcc aatgccattg acctggtgat tttcgatgac gatctcagcg
     2941 gcaaacaaac caataccata gaagaggccc tgaaggtaaa aatcgtggat cgttcatccc
     3001 tgatccttga catatttgcc aaaagggccc agacggccca ggccaaaacc caagtggaac
     3061 tggcccagat gcaatacctg ctgcccaggc tgcgcggact ctggacccac ctggaaaggc
     3121 aaaggggagg gatcggcatg agggggcccg gtgaacagga agtggaaacc gaccgccggc
     3181 tggtaaaaaa taaaatttca atcctgaagg aaaagctgga aaaaatagac cagcaggctt
     3241 ttacccagcg ccgcagccgg ggggaattga tccgggtagc cctggttggc tataccaatg
     3301 tgggcaaatc caccctgatg aacctgttga gtaaatcgga cctcctggcc gaaaacaaat
     3361 tatttgccac cctggatacc accgtgcgca aggtttattt cgaggatatg cccttcctgc
     3421 tttcggacac ggtcggtttt atccggaaac tgccccacca cctggtcgaa agtttcaaat
     3481 ccaccctgga cgaagtccgg gaaagcgatc tgatcctgca cgtggtcgat ctctcccatc
     3541 cccaatatga agaccagatc ggcacggtca acaagaccct ggaagaactc ggcgtttcct
     3601 caaaatccat cctcatggta ttcaataaaa tggatgccta ccgggaaaaa tatttcgacc
     3661 ggctgctcag tccggaagaa aaaaaggaac tggaagacca actggtacaa atgttgaaaa
     3721 gccgttatcc cgttcccatg gtggtgattt cagcagccaa aaaggaaaat atggaggaat
     3781 tccgcaggat cctgacccgg atggtaaaag aacaatatgt agtccgctat ccatacatgg
     3841 tgaaaagctg gtagggatgc atcaaaaaca attggaggct tacagccgcc tgctgctgga
     3901 ttattccctt cgcctcggcc gcggcgacaa actgtatatc cagaccacta cgctggccct
     3961 tcccctggtc cggcagcttt atgagggggc gctcaggcgc ctggccgtcc cggtgattca
     4021 catgagtttc gaaggacagg aagaacttca aaaccagctg gcgcacgaag aattcatcag
     4081 gtatatcaat cctgcttacg ccgaagcggt cgaacaattc cacgcttttt tgttcatccg
     4141 ggctccgttt gatgaccggg gctttcctcc catggacaaa ggccttcagg ccctccgcag
     4201 ggaggtgacg ggccctttgc aaaagaccta tgccatacga accggcaacc agtccctgcg
     4261 gcgcacgctt tgccagtatc ctaccccggc cggagcgaag gaagcttcga tgactacgga
     4321 ggaattcggc gattttattg ccgctgcctg ttttttggac gaaacccgtc cggaagaaag
     4381 ttggaaacag ctcggccggg aacaacaggc ttatgtcgat ttcctgaatg gggtgaagca
     4441 gattaccttc aggaatgaaa agacggagat gacctgctcc gttgacggac gtacctggat
     4501 caattcggat ggcaaaaaca atatgcccag cggcgaggtc tataccagcc cggtggagga
     4561 ttcggtcgag ggccggatct attttgatta tccgtttacc tatgaacatc atacgattcg
     4621 cggagtaagc ctcagggtgg agaaggggaa gatcgtggag gccggcgccg aagaaggaaa
     4681 cgaaaaactt caggaaatcc ttgaaattcc cggagcacgg agttttggcg aaatagccat
     4741 tggcaccaat acgcatattc aaaggccgac ctgcaatatc cttttcgatg aaaagatggc
     4801 gggcaccgtg catatggcca tcgggcaatc ctacctgcaa tgcggaggca aaaatgaatc
     4861 ggcggtccac caggatatga tcgctgacat gcgcagggga gggcagatct gggcggatgg
     4921 aaggctgatt tatgaaaatg gggttttttt ggaagttgga agttagaagc tagaagctag
     4981 aagttagaag ttagggcgtt ttggcctggc agttccggag gtttagaagt ccctcctcta
     5041 gcttccagct tccaagttag aagctagaag ttagggcgtt ttggcctggt agttccggag
     5101 gtttagaagt cccacaccta gcttcgagcc cccagcttcc agtttcgagc ttccagcttc
     5161 cagcccccag cccccagctt cgagcttcca gcttccagct tccagccccc agcttcgagc
     5221 ccccagcccc cagcttcgag cctccagccc ccagcttcca gcccccagcc cccagcttcc
     5281 agctcccagc ttccagcttc cagcttcgag cttccagttt taatacttcc ctaactaaca
     5341 ttcctgtttt ttccattgtc tttatcatat cagaccatta aaaccggatg ttatgaaaaa
     5401 caagttgaat ttgctgctcc tctcttcgat cctggcgctc ggcgcctgtg aaagagacct
     5461 cggcgaatat gtaatcattc ccgtgaatac aggcccattc agccaattgc gccaggatgc
     5521 gtctacggat attgtcctca ggcaaggtcc cggattgttg gtagaatttg aagggtatga
     5581 agacatcttt tatgacctgg atttcagggt ttccgggggc aaattgatca ttagccagcg
     5641 ggggcactcc tggtcttccg ggcgctccaa aatatatatt acggtacctg atctgaccaa
     5701 aattgaaatt acggcgtccg gagatgttta tggcgacaac cggttccgga tcaacgggga
     5761 tattgaactg cattcccgcg caagcggcga tttcgacctc aatctggatg tggatgaggt
     5821 gtatacctgg attgaagggt cgggccaggt ccggcttagg ggttatgcgg acctgcttga
     5881 agcggagatc gacggttccg gggacttgta cgcttttcca ctaaatagcc ggaacacccg
     5941 gatcaccagt tatggaagcg gggatgccga aatttatgcc acccacaacc tgtatgtaag
     6001 gctccgcggg agcggggata tctattttac cggttatccc tatgtggact atctggtgac
     6061 gggttcggga aggctgatcg atgccaattg atcccgtgtt aaatccatgc ccccgaaatc
     6121 tcaggcacac tgccccgttt acaaatcatt aatccggggc agtttcagta attaaattaa
     6181 ataataatat atacacccct taatatcaaa tattaggacc ctataccacg ggatttaaag
     6241 gggagtttag aaaagaaatg atttttttgc ggatatttcc acttgaataa gaaaaataaa
     6301 gtgtaatttt gccccgctca aaccgattgg ttcattaaag tcatctaata agcatgctgg
     6361 aagaaaaaga cgaaatccag gatatggaaa aaaacatacc ccctgccaag gaagaaactg
     6421 aggaccagac cggttctgcg gagcctgaaa tggcagagga agacggtgcc ctgatagaag
     6481 taccggaact ggagcttgag ctggaagccg tccaggagga gaatactgcg catgatgatt
     6541 ttaactggtc tgccggaaac cggaatacaa gatattattc ggacgaagag aaaaaatcgt
     6601 atttaaacaa atacaccacg accctcagta cgcttgggga tcatgaaatc ataaaaggta
     6661 aagtctcctc catccacggc tcggacgtgg tgctggacat caactataaa tccgacggcc
     6721 tcattcctta tgatgaattt aaggatatgg gggacctgaa agtgggtgac ttcgtagacg
     6781 tttacgttga atcccaggaa aacgccaaag gccagctggt cctttcccgt aaaaaagcca
     6841 agttgctgaa agcctgggaa agcctggtcg attctttcaa caatggcacc atcatcaaag
     6901 ggaccgtggt cagcaagacc aaggggggcc tgatcgtgga tgccagcggg ttggaaacct
     6961 tcctgccggg ttcgcagata gacatcaaac cgatcaccga ctatgatcaa tacgtcggca
     7021 agaccatgga attcaaggtg gttaagatca acgagaccat aaagaatgcc gtgatctctc
     7081 acaaagccct gatcgaaagc gacctggctg aacaaaggga tgcgatcata tccggtctgg
     7141 aaaaaggcca ggtactcgaa ggcgtggtca agaacatcac cgacttcgga gccttcatgg
     7201 acctgggcgg ggtagacgga ctattatata ttaccgatat ctcctggggc agggtcaacc
     7261 atcccaacga ggtattggcc atgaaccaaa agctcaatgt ggtggttctg gacttcgacg
     7321 aaaacaaaaa gaggatttcc ctgggcttga aacagcttca gcctcatccc tgggatgtgc
     7381 tgggcgatac catcaaagaa ggggatgtcg taaaaggcaa gatcgtcaat atcgaagact
     7441 acggggcatt cctggaaatc agtccgggca tcgaaggcct catccacgtt tcggaggtga
     7501 gctggtccaa ccagcctgtg aattcaagag attatttcca gcttggccag gaattcgaag
     7561 ccaaggtggt tacgatagat aaagaggaaa ggaaaatgtc gttgtcgctg aaacaactca
     7621 gccaggatcc ctggacgacg atttccgaag cctacccggt gggcagcagg cataccgggg
     7681 tagtgaaaaa ccttacgccc tacggtgttt ttgtagaact gggtgagggt atcgggggca
     7741 tgatccacat cagcgacctg agctggacca agcggttttc ccatccttct gagtttatca
     7801 gcatcgggga gaagattgac atcatggtcc tggaaataga caaggacaac cggaaattat
     7861 ccctgggcca caagcaggtg gaagaaaacc cctgggatac tttcgagcag gtgttccccg
     7921 tcggttccta ccatgaagcc acggtgatcc ggaaggacga caaaggaggc gccattgtgc
     7981 agctgcctta cggcctggaa gcctatgccc cgcagaagca catcaagaag gaaaacggat
     8041 cttatgcgga agtggacaat gtgctgacct ttaaagtgat tgaattcaac cgggacgaca
     8101 agcggatcct ggtttctcat acccgtttcc tggacgatat ccggaaggag gccagtaagg
     8161 ccgtaaaagg aagcggtgat caggcgacgg acgaggctga aaagaaagcc gcgttaaaag
     8221 ccgcaaaagc cttgacaaca aaggctgaaa agactacttt aggagaattg gcggagtttt
     8281 cggctctgca gcaaaaattg aagggtaata tttctccgga accgacggac aagtcggaag
     8341 aggagaaaga aaaataaata aaaatatttt tcagaaactt tttggattag taaaaagttt
     8401 tatatctttg cgtgccgcaa tcaaaaggag agcggttgag agatgaaaaa tctctgaaaa
     8461 aacaagttca ttgacaagta gggagaaaga ggaaggaaga gtactatacg aaatgaatga
     8521 gtcatgacaa tggagagttt gatcctggct caggatgaac gctagcggga ggcttaatac
     8581 atgcaagtcg aacggcaaca tgatgatagc aatattattg atggcgagtg gcgcacgggt
     8641 gagtaacacg tacataacct gccctttact ggaggatagc cttgggaaac tgggagtaaa
     8701 ccttcatagt gtataccgga cgcatgtctg gtataataaa ttcttcggga ggtaaaggat
     8761 ggatgtgcgg ctgattagct agttggtgag gtaacggctc accaaggcga tgatcagtag
     8821 ggggtgtgag agcatgatcc cccacacggg tactgagaca cggacccgac tcctacggga
     8881 ggcagcagta agg
//
LOCUS       DAOQXT010000151         8853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_8387_length_8853_cov_6.805979, whole genome shotgun sequence.
ACCESSION   DAOQXT010000151 DAOQXT010000000
VERSION     DAOQXT010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8387_length_8853_cov_6.805979"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..830
                     /locus_tag="PKM27_17920"
     CDS             72..830
                     /locus_tag="PKM27_17920"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HNR09203.1"
                     /translation="MLALQWIDRAKKVMDTIEASQLENIRKAAAAMADSIGAGRWVHT
                     FGCGHATIPVEEMYPRIGGFVGFHPIIELPLSFFTHIVGDMGIHQFLFLERTEGYGRE
                     IMKSYDFDSRDCMWIFSHSGINNLNVDVALESKKRGMTVIAYGSAEASRVKQPRHSSG
                     KSLFEVADIVVDSCAPLEDAAVSLKHHVDKVGPVSTLAFVTTVWLTIITVAEILEERG
                     VKLFIHPSHNVGDDTAMDRLHEALEEYRRKVKLL"
     gene            complement(856..1869)
                     /locus_tag="PKM27_17925"
     CDS             complement(856..1869)
                     /locus_tag="PKM27_17925"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HNR09204.1"
                     /translation="MNMQVSTLKNILVLASHLIGIPENKLLSSLGLNAGEFEDNNKHL
                     DWKKGAKALSKVALLARDPKLGIRIGTLTMLQVSGNELSNLIVHSPDYLAAVRNFCEF
                     SRIIGSMYHFTFTVKNDVCTIRFTHGIPEWKDEDLISYRLLTEISMTSMINLLKQLSQ
                     GRIRPLALEFDYTLDPEVRKGFKEMIDIPMLEDQKENALLLAKKELVLKNPNYSEVYY
                     KMALAEATDRLKEVEQGQEIPELVRVEIRNILKDPNENLKASLEEVAARMNRSSRTLQ
                     RELKKEGMSFEQLKTEVSKEFVDRLLQSRKKITPGELADKIKLSLTAFREWYKRNYGR
                     EFT"
     gene            complement(2167..3987)
                     /locus_tag="PKM27_17930"
     CDS             complement(2167..3987)
                     /locus_tag="PKM27_17930"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HNR09205.1"
                     /translation="MVLIKRRNKINLLLALFIAGWGVLRAEESIRFTVSMPDPSSHRF
                     HVRMQYEGYSIADSLEFHMPVWTPGYYQRLDFWKQVSHFKALDGLTGHALDWNQGQNV
                     WKVSTGRTGSLVLEYEVLTLRPFVAQPWLDTARGYVLPAGLCVYPAGKIRLPVLLEVE
                     PYGQWTTATGLKGIRGKTNTYYASDFDVLFDSPVLAGPIESLPSFRVRGVPHYFKGIQ
                     LGTFDKQKFIGDLKKIVETAVDLIGHIPYEDYTFLAIGPGAGGIEHLNNTTFSFNGNN
                     LHDRSAYLRMLFFLAHEYFHHYNVKRIRPIELGPFDYDQGNRTHSLWWSEGASVYYEY
                     LVVKRAGLCTEEEMLDALRKNIAAFEDKPGKAFQTLAQASWATWSDGPFGRVDDEINR
                     TISYYDKGPVVSMLFDFRIRRMTGNQKSLDDVMRDLYYRFYLNRKRGFSEFELKQSME
                     RVAGGRMDDLMDYVYTTRDLDYRASLAEAGLYMDDTLRQTRGGWTGMSTRRREDTLQV
                     FSVEYESPAWKNGFRRGMKILRVNDKVGFAIEPVMPELKPGETLKIEILEEGNLSDRI
                     LVLGQKTSKSYLLKPLPKPDGLQKAIYKSWMGQGGGRKGL"
     assembly_gap    4190..4289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4290..5842)
                     /locus_tag="PKM27_17935"
     CDS             complement(<4290..5842)
                     /locus_tag="PKM27_17935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR09206.1"
                     /translation="MNEFEKLGVDAQLTKAIQEIGFEKPTEIQEKAIPLLLTGTRDFI
                     GLAQTGTGKTAAFGLPLLQLTDPSKKHPQALVICPTRELCMQISKDFISYKKHKAGIQ
                     TCAVYGGASISEQLKEIKKGVQILVATPGRLIDVIDRKAIDLSHIHYIVLDEADEMLN
                     MGFREDIEYVLKHTPERQSSWLFSATMPPEVREVSKRFMKTPHEVTIGKVNMASSSID
                     HQYFLVLHSNRYEALKRLIDFNPGIYGLIFTRTKLDAQQITESLVREGYDVDALHGDL
                     SQPQRDKVMARFRTKQLQLLIATDVAARGIDVKGISHVINYELPDDPEIYTHRSGRTG
                     RAGLTGICMSIVTPKEKSRMDQIERFVKAPFHKLDIPSGTEVCRKQFFHFIDKMFAAD
                     ISHGEYETYLSALKERFENVDKEEIIKRMAALEFNRFLQYYENAADLNIRKEEKAGKK
                     KDKFKKGGEPGREGFAKGGKYKSLFINLGQKDGFYKASFLQFVLDSSNLRKEVLGKID
                     MKNLNTILE"
     gene            complement(6003..8483)
                     /locus_tag="PKM27_17940"
     CDS             complement(6003..8483)
                     /locus_tag="PKM27_17940"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNR09207.1"
                     /translation="MKFVFVPIVFAFCSVYCTAVHSKDSSPRELHAIKCEEVPVIDGV
                     LDDAAWKQAQEITDFTEFRPKIGDKEAHAERTVAYLMYNDIGIYFGGRCYEPSTDRIS
                     KELQGRDGFGANDYIGIIFDTYKDNLNGFEYFVTPLGEQWDAKMAPANNSNNGGEDFS
                     WNAVWNSAARIHDEGWDFEMFIPFSAIRFGSGDVQDWGLNITRRRRLTEEQFTWNPID
                     PTVNGFLTQEGYWKGIKNIKPPVRLQFSPYFSIYANHYPSQDPATRDWSSQVNGGLDL
                     KYGINQAFTLDAVLIPDFGQVQSDNQVLNLSPFEVKFNENRNFFSEGTELFNKGGLFY
                     SRRIGGTPLHHGRPGESLRENEYILKNPAETKLINAAKISGRSQSGLGIGFLNAITKP
                     QHAVIENRSSGETREETTNPLTNYNFMVLDQTLKNNSSVTLVNSSVMREGRDYDSNVS
                     AFLFDFNDKSNTWNVNGKVALSQYFQSGEENRPGTAYNLGFGKVSGQFSFRFWQEYAD
                     ERYSHNDLGYFTNNNFLSHGVYGSYRIIKPKGWYNRINFNFNTSISHLASRIGDIDAR
                     YQSANVNMNINAQTKKLLFVGLFTGYQPARNDFWEPRVTGQYFRRGPRAHAGLWLETN
                     SAKKYSFYTEVFGGRFINFYDINIFEGQLSQTLRFSSRFSLSHFISYEHVTNSVGFAT
                     QLSSGEVVMGRRNINTVSNIVSSKYNFNTKMGITLRVRHYQSGVDYNSYYHLMSDGSL
                     SPNESFRENVDQNVNFFNVDMVYTWQFAPGSFLNLVWKDAAFSSEDRNESRYVRNLNN
                     ILDSDHNNNLSLKVIYFLDYLQLKKRTG"
BASE COUNT         2061 a         2116 c         2279 g         2297 t
ORIGIN      
        1 tcgctgctct tccttttctt ccgaagccat aaagaaaata tatattcaca atcaaaaaac
       61 aatcaataaa aatgctggca ttacaatgga tcgaccgggc caaaaaagtg atggacacca
      121 tcgaagcttc gcaactggaa aatatcagga aggcggccgc ggcgatggcc gactccatcg
      181 gggcgggacg ctgggtgcat acttttggct gcggccatgc caccatcccg gtggaggaaa
      241 tgtatccgcg catcggcggt tttgtgggtt tccatccgat cattgaactt cctttgtctt
      301 ttttcacgca tatcgtcggg gatatgggca tccaccagtt tttgttcctg gagcgaacgg
      361 aaggctatgg ccgagagatc atgaagagtt acgattttga ttccagggat tgtatgtgga
      421 ttttttccca ttccggcatc aataatctca atgtcgatgt ggcgctcgag tccaaaaaaa
      481 ggggcatgac ggtgatcgct tacggctccg ccgaagcgtc cagggtaaag cagccccgcc
      541 attcttccgg gaaatccctg ttcgaggtgg cggatatcgt ggtggactcc tgcgcgcctc
      601 tggaagatgc ggccgtatcc ctgaaacatc atgtggataa ggtcgggccg gtttccacgc
      661 ttgccttcgt caccacggtc tggctgacga tcatcacggt agctgaaatc ctggaagagc
      721 ggggtgtcaa attgtttatc catccctcgc acaatgtggg cgatgatacg gccatggacc
      781 gtctgcatga agccctggag gaataccggc gaaaagtcaa attactctaa gcccagccag
      841 gaaatacaga ccctgttagg taaactccct tccataattc cgcttgtacc actcgcggaa
      901 ggcggtaagg gaaagtttga ttttatccgc cagttctccg ggagttattt ttttcctgct
      961 ctgtagaagc cggtccacaa attctttact gacttctgtt ttaagctgtt caaaactcat
     1021 tccttccttt ttcaattcgc gttgcaaggt cctggaagac cggttcatcc tggccgctac
     1081 ctcttccagg gaagctttga ggttttcatt cggatccttc aggatattcc tgatctcgac
     1141 gcggaccagc tccggaattt cctgaccctg ctccacttcc ttgaggcggt cggtagcctc
     1201 tgccagggcc attttataat aaacttccga ataattcggg ttcttcagga ccagctcctt
     1261 tttcgccagc aaaagggcat tttccttctg gtcctctaac atcggaatgt caatcatttc
     1321 cttaaagccc tttctgactt ccggatcgag ggtataatcg aattcaagcg ctaagggccg
     1381 gatcctgccc tgggacaatt gtttcaggag gttgatcatg gaggtcatgc tgatctccgt
     1441 aagcaggcgg taagagatca gatcctcgtc tttccattcc gggatgccat gggtgaaacg
     1501 gatggtacaa acatcgtttt taactgtaaa cgtaaagtgg tacatggaac caatgatccg
     1561 gctgaactcg caaaaattcc ttacggcggc caagtagtcg gggctgtgga cgatcaggtt
     1621 gctcagttca tttccggaga cctggagcat ggtgagcgtt ccgatgcgga tgcccagctt
     1681 tggatcgcgg gccaggagcg ctaccttcga aagggccttg gctccttttt tccagtccag
     1741 gtgtttgtta ttgtcttcaa attcgccggc attgagtccc aggctggaga ggagtttgtt
     1801 ttccggaatt ccaatgaggt gactggccaa caccagtatg tttttcagcg tggaaacctg
     1861 catgttcata ttatccgcat tgatataaaa ttatttattt tatgaaaaag ggtttgaaat
     1921 cgggtggacg aagaattaaa aattgtcacg aaaccgggat aggttgacgt aaaaccgtat
     1981 gctgaatatg acgaaatttg cggcctcaat tgaattaacc ctttatacta attcattggt
     2041 ggtcaatcag atacgatgtt tgaggagcag ctgaccactg agattggcta cggcggcaac
     2101 gtcctacttc agggggaaca aatagttcct gcttttaaag aaagaagttg ccgccgacac
     2161 accaatttac aggccttttc gtcctccgcc ttgccccatc caacttttat aaatcgcttt
     2221 ttgcaggccg tccggtttcg gaaggggctt taataaatac gattttgaag ttttctgacc
     2281 caagactaaa atccggtcgg aaaggttccc ttcttccaaa atttcaattt ttaaagtttc
     2341 ccccggtttc agttctggca tcacaggttc aatggcaaaa ccaaccttat cgtttacgcg
     2401 caggattttc attccgcgtc tgaacccatt tttccatgcg ggagattcgt attccacgga
     2461 aaacacctgg agggtatcct cccgtcgccg ggtactcatg cccgtccacc ctccccgggt
     2521 ttgacgcagg gtgtcatcca tgtacaatcc agcctccgca agactggccc ggtagtccag
     2581 gtcgcgggtg gtatagacat aatccatgag gtcatccatc cttccgccgg cgaccctttc
     2641 catgctttgt tttaattcga attccgagaa accgcgtttc cggttcaggt aaaaccggta
     2701 atacaggtct ctcatcacat catccaggga cttttgatta cccgtcatcc ggcgtatcct
     2761 gaaatcaaac agcatcgaaa ccaccggccc tttgtcgtaa taagaaatcg ttcggttgat
     2821 ttcatcgtcg acccttccga aggggccgtc gctccaggtc gcccagcttg cctgggccag
     2881 ggtttgaaag gctttacccg gtttgtcttc aaaagccgcg atgtttttac gaagcgcgtc
     2941 cagcatttct tcttcggtac agagccccgc ccttttgacg accaggtatt cataatagac
     3001 cgatgcccct tccgaccacc acagcgaatg ggtccggttg ccctggtcat aatcgaaagg
     3061 acccagttca atgggccgta ttcgttttac attgtaatgg tggaaatatt catgggccag
     3121 gaaaaacaac atccgcaaat aggccgaacg atcgtggaga ttgttcccgt taaaactgaa
     3181 agtggtgttg ttgaggtgtt caatgccgcc ggcgccggga ccgatggcca ggaaggtata
     3241 gtcttcataa ggaatatgtc ctatgaggtc caccgcggtc tccactattt ttttcaagtc
     3301 cccgataaat ttttgtttgt cgaaagtgcc caattgaatt cccttaaaat aatgaggcac
     3361 tccccggacc cggaaggagg gaagggattc gatggggccc gccaggacgg ggctgtcgaa
     3421 caggacatca aaatcggagg cataataagt atttgttttg ccgcggatgc ctttcagacc
     3481 ggttgccgtc gtccattgtc cataaggctc cacttcgagg aggacgggca ggcggatctt
     3541 tccggccggg tatacgcaaa ggcctgcggg aagcacatag cccctggccg tatccagcca
     3601 gggttgggct acaaagggac ggagggtcag cacctcgtat tccaggacca gggagcctgt
     3661 ccggccggtt gaaaccttcc agacattctg cccttgattc cagtccagag catggcccgt
     3721 aaggccatcc agagccttaa aatgactgac ctgcttccag aaatccaggc gctggtaata
     3781 cccgggcgtc cagaccggca tgtgaaattc caggctgtcc gcgatggaat atccttcgta
     3841 ttgcatccgt acatgaaaac gatgcgagga gggatccggc atcgatacgg taaaccggat
     3901 ggattcttcg gctctcagga ctccccaacc ggcaatgaac aaggccagta aaaggtttat
     3961 tttattcctt ctctttatta ataccataac cttggataaa caaacttccg gctaacttac
     4021 tcatttattt atttatgaca gaagcgagac acgagcagcg agaagcgaga agcgagacac
     4081 gagaaacgag aagcgagaga cgagcagcga gacacgagaa gcgagaaacg agataaactt
     4141 gcggacttat gaacctgcta acctgttgct gactactgac tactgactan nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tttccaggat cgtattcagg tttttcatat
     4321 ctattttccc caggacttct ttcctcaggt tgctgctgtc gagcacaaac tggagaaaag
     4381 aagccttgta aaacccgtct ttctggccga ggttgataaa taaggattta tattttcccc
     4441 ctttggcaaa tccttctctt cccggctccc cgcctttttt aaatttatct tttttctttc
     4501 cggctttctc ttccttacgg atattaaggt ctgcggcgtt ttcataatat tggaggaaac
     4561 ggttgaattc gagggcggcc atgcgcttga tgatttcttc cttgtccacg ttttcaaatc
     4621 tttccttcag ggcagacagg taggtttcat attctccgtg gctgatgtcc gcggcaaaca
     4681 tcttgtcgat aaaatgaaaa aactgtttgc ggcatacttc agtaccggag ggaatgtcca
     4741 gtttgtggaa aggggctttt acaaatcgtt cgatctggtc catccggctt ttttctttcg
     4801 gggtcacgat gctcatgcag atgccggtga ggccggcccg gcccgtacgc ccgctgcggt
     4861 gggtatagat ttcggggtca tccggcaatt cataattgat cacatggctg atccctttga
     4921 cgtcgatccc cctcgcggcc acatccgtgg cgatgagcag ttgaagttgt ttggtacgga
     4981 accgggccat gactttatcg cgctggggct ggctgaggtc tccatggagg gcatccacat
     5041 cataaccttc gcgcaccagg gattccgtga tctgctgggc gtccagtttg gttcgggtga
     5101 aaataaggcc ataaattccc gggttaaagt cgatcagccg cttaagcgct tcgtaccggt
     5161 tgctgtgcag gacgaggaag tactggtggt caatggagga gctcgccata ttgaccttgc
     5221 ctatggtgac ttcatgaggg gttttcatga atcttttgga cacttcccgc acctcgggag
     5281 gcatggtggc gctgaaaagc cagctgctct ggcgctcggg ggtatgcttg aggacatatt
     5341 ctatatcctc cctgaaaccc atattgagca tttcatcggc ttcgtccagg acaatataat
     5401 ggatatggct caggtcgatg gccttgcggt cgatcacgtc gatcagccgg ccgggggtgg
     5461 ccaccaagat ctgaactcct tttttgattt ccttcaattg ttcggatatg gacgcccctc
     5521 cgtacaccgc gcaggtttga attcccgctt tgtgcttttt atagctgata aagtccttgc
     5581 tgatctgcat acaaagttcc cgggtcgggc agatcaccag ggcctgggga tgtttttttg
     5641 aagggtccgt aagctggagc aggggcagcc cgaaagcggc cgttttgccg gtgccggttt
     5701 gggccaggcc gataaagtcc ctggtcccgg tcagcaggag tgggatggcc ttttcctgaa
     5761 tttcagtggg tttctcgaag ccgatttcct ggatggcttt ggtcaattgg gcatccacac
     5821 ccaatttttc gaattcgttc acaaataaga attgttggaa aacaacctgt ttccggctgc
     5881 aaaggtaaga attaaaggag gaagggagac gggaggaagg gagaaaggta aaaggtaaaa
     5941 ggtgaaggga gcaaaaggcg aaagggagaa aggtaaaagg agaaaaaagc ggaccaggta
     6001 gtctatccgg ttctcttttt caattgcagg taatccagga aataaatgac tttcagtgac
     6061 aggttgttgt tatggtctga atccaggatg ttgttgaggt tcctgacgta gcgggattca
     6121 ttccggtctt cgctggagaa ggcggcgtct ttccaaacca ggttgaggaa gctgcccggg
     6181 gcgaattgcc aggtatagac catatcgaca ttaaaaaagt tgacgttctg atccacgttt
     6241 tccctgaagg actcattggg ggaaaggctg ccatcggaca tcaggtggta ataggaattg
     6301 taatccaccc cgctttggta atgccttacc cgcagggtga tgcccatctt tgtattgaaa
     6361 ttgtatttac tgcttacaat attggaaacc gtattgatat ttctacggcc catgaccacc
     6421 tcgccggaag aaagttgggt ggcaaaaccg acactgttgg tcacgtgttc ataactgata
     6481 aagtggctga gggaaaaccg ggaagaaaaa cggagggtct ggctgagctg cccttcaaag
     6541 atattgatat cataaaagtt gatgaagcga ccgccgaaca cttcggtata gaaagaatat
     6601 tttttggcgg aattggtttc cagccatagt ccggcatggg ccctgggacc ccggcggaaa
     6661 tactgcccgg tgacccgcgg ttcccaaaaa tcattccggg caggctgata tccggtgaac
     6721 aaacccacaa aaagcagttt tttggtttgt gcattgatgt tcatgttgac atttgccgac
     6781 tggtaacggg catcaatgtc cccgatccgg ctggccaggt gggaaatgct ggtgttgaag
     6841 ttaaaattga tccggttgta ccagcctttg ggtttgatga tccggtagga gccatagacc
     6901 ccatgactga ggaaattgtt attggtgaaa tagccgaggt cattgtggct gtatcgttca
     6961 tcggcatatt cctgccaaaa gcgaaaactg aactgaccac tcactttgcc aaaacccagg
     7021 ttgtacgcag tgcctggccg gttctcctcc ccgctctgaa aatactggct cagggcaacc
     7081 ttgccgttga cgttccaggt attggatttg tcattgaaat caaagaggaa agcgcttaca
     7141 ttgctgtcgt agtccctgcc ttcgcgcatc acgctggagt tgaccagggt aaccgatgaa
     7201 ttattcttca gggtctggtc caggaccata aaattataat tggtgagggg attggtcgtt
     7261 tcttcccggg tttctcccga acttctgttc tcaataaccg cgtgctgggg tttggtgatg
     7321 gcatttaaaa aaccaatccc cagccccgat tgagagcgtc cggatatttt ggctgcgtta
     7381 atgagtttgg tctccgccgg gtttttcagg atgtattcat tttccctcag ggattctccg
     7441 ggccggccgt gatggagggg tgtcccgcca atcctcctgg agtaaaaaag tcctcctttg
     7501 ttaaacagtt ccgtaccctc gctgaagaaa ttcctgttct cgttaaactt cacttcaaaa
     7561 ggggagaggt tcaggacctg gttgtcgctc tggacctggc caaaatccgg aatgaggacg
     7621 gcatcgaggg taaaggcctg gttgatgccg tatttcaggt ccagtcctcc gttgacctgg
     7681 ctgctccagt ccctggtcgc cggatcctgt gaagggtaat ggttggcata gatggagaaa
     7741 taaggggaga attgcaggcg caccgggggt ttgatgtttt taatcccttt ccagtagcct
     7801 tcctgggtga ggaatccgtt gacggttgga tcgatcgggt tccaggtaaa ttgttcttcg
     7861 gtcaaccttc tcctgcgggt aatgttcagg ccccagtcct gcacgtctcc gcttccaaaa
     7921 cggatggcgg aaaatgggat gaacatttca aaatcccaac cttcgtcgtg gatccgcgcg
     7981 gcgctgttcc acacggcgtt ccatgaaaaa tcttctccgc cgttgttgct gttattggcc
     8041 ggcgccattt tggcgtccca ttgttcgccg agcggggtga caaaatattc gaaaccgttc
     8101 aggttgtctt tgtaggtgtc aaaaatgatg cctatataat cgttggcacc gaaaccatcc
     8161 cggccttgca attctttgga aatccggtcc gtggagggtt cgtaacagcg gccaccgaaa
     8221 tagataccga tgtcgttgta catgagatag gcgaccgtgc gctctgcatg ggcttccttg
     8281 tccccgatct tcggcctgaa ttccgtaaag tccgtgatct cctgggcttg tttccaggcg
     8341 gcatcgtcca acaccccgtc aataaccggc acctcctcgc attttatcgc atgaagttct
     8401 cgcggggagc tgtcttttga atggaccgcg gtgcagtaaa cggaacaaaa agcaaaaaca
     8461 atgggaacaa atacaaactt catgcgaccg cttaatttat tacagtaact ccaaagacca
     8521 aaaatagtta ccggggttgc atagctacaa tggttttgaa agatgtaaca gttctttaac
     8581 gggttttgag ctgtggtttc gaactgtttg aatttttaac cgcgaagaac gcggagtacg
     8641 cagaggatcc gcagaggatt aatgaaccgc ggagtgagcg tagcacgtta aggatttata
     8701 aaccgcgaag agcgcggagt acgcagcggt tccgcaaagg catgtattgg catgaagtcg
     8761 ctgagactgg ccccaagtgc aattccgcag cccaggggca gtcggaggcc ggaaggcctt
     8821 tactttgtgg ccttggtgtc ttggcgggat att
//
LOCUS       DAOQXT010000152         8829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_8417_length_8829_cov_5.265215, whole genome shotgun sequence.
ACCESSION   DAOQXT010000152 DAOQXT010000000
VERSION     DAOQXT010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8417_length_8829_cov_5.265215"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="PKM27_17945"
     CDS             complement(<1..1066)
                     /locus_tag="PKM27_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNR09208.1"
                     /translation="MSPTLPEQELIRRETLTEIREAGIDPYPAELIPVTGFTADILRL
                     FDDDPGRYQSVRLAGRLMLKRSMGKASFAELMDARGRIQLYLKKDELGEAYDPLFSKW
                     LDLGDFIGVEGYVFRTKMGEITVHVREIRLLSKSLKVLPVVKTDAEGKVHDAFTDPEL
                     RYRMRYVDLLVNPGIRDIFLKRSKLLSALRRHFSEKGWLEVETPILQSIHGGAAARPF
                     MTHHNTLDIPLYLRIANELYLKRLIVGGFDGVYEIGKMFRNEGMDRTHNPEFTMMEIY
                     VAYKDYYWMMEMVEELLETLALAVHGQTDIPYGEHLLQFKGPYRRLSMHDAIKEYTGV
                     DVSTMDEAALRQQCKAWHIET"
     gene            1650..6746
                     /locus_tag="PKM27_17950"
     CDS             1650..6746
                     /locus_tag="PKM27_17950"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR09209.1"
                     /translation="MFANRSECTADCERIISGIIGNCNFAIRYNPFELSTNTGAFILV
                     ENNNTGIAAESTGCQLYALNCEAPVIDVLPNVDSTACAIDGFELQPFTVDPVFSAKYF
                     TLPNRRGDSFNIGQIITQSIKLYAHAYADCNPALSVQDSINIIINQGPQIPFQSTQAC
                     GFHVLPPFAGTNLSPNVKYYTGDFGTGTQYLPGDIIRSSVATTIRLYAYDVDTKGCAA
                     EGGYFIQFLNPPTIVNPKDTTVPCRANYLFPAITGTALTPLRAYYTESMGGGNRIGQL
                     TPATMSGTYFVYDTFYNCFDEDTFRVTLIPVTQIPKDTIRNDTFCNQLILPDIQSASL
                     IYNTARNFTGVNSNPGDILNASTKLYVKATAMGCETIDSVQYTYRELDLDPITIDEPC
                     KPLLDALPPITGANLTAQVAYWTLPGGKGQRYLPGDSVLAAGAGGIYQSTTLYAFDSV
                     SYLSGSCTVERTVEIPFHTIVELTSISDTVLACGQTLEVPLVKPDQVYQGMVYPDRAL
                     TSALIPGSSIPQSGTYYVYASYLSCSDLDSFSVQFNTGPQYFNNIDLSDPCSEVTLLP
                     VNGTDFNPDSTFYFRGTFGTGDRFSPGMTVNQADTFYIFDLSQPMCLVQDTVVVSFNS
                     QQGFNLPASPWDACDSIRLPNDPTVGNLQYIVQNGDAVDIKFPGDWITSSMRVEAVGG
                     DPNCPRRETITIRIAQTPELVPMRDTLLCDPLRLPVLTGKNLADPHYHTLPNDGGESL
                     YAGDTVFQTGTYYPFSRNGACVDEDTVQITIVNRPRINKLTDQRVCEKYILPLPAGAN
                     LDSLYYYDDRSRQGERYAIGDTLRRSMKIYAYQDVPLCPAIDSMVITVLDSTTTEFML
                     SDTLFCVGTPLLANHTGDRGPETTFTWAFSGSTQVTFKGGNADQRWDLGPGNYNITLS
                     GETGSCDGPPTTHSVRIHDTLAALTGLNCTRGPDNIAFRWNQVPGALEYGIELLAGPT
                     GVRTPFEMLFSNLAPGQPVGIRVTPLGGIPCANGQADSLVCFPTVLCEPVTVEITQEG
                     PFCSGDGPIEPVALIRGASDPASYTRTWSGPGMVRDTFYPAQAGPGEHIITFTLTRDS
                     CIFTDTVVFRVGGGSVQILNDQGTICNPADQDQFNIRMFIDAFDRPYDVHYTYNGRPS
                     AFPSGSDTFTLSVRSGVIRIDSVVDASGCKLPINSMAGTDSFRLVQPLAPVDTQFICD
                     FGRRAWSYQVVINNPNPAEPMLVLSGGGTFQDSLYTSPPVPFGQRHLARISRDAACDT
                     LVFDLTFSCDCTPHRDTVPTAVQCEGDTVVIGNRTYTAGNPFHIDTIPPAVIGACDTF
                     RVVNITFLPRGRQVLNPVFCPGDTLRVGNVVFGHGNPIGEVYLPGMAANGCDSLVVVQ
                     GLALNPVTVIIRDTICAGDTLRLSGIEFHNGHRADSVRLAGGAANGCDSVVYFFIHVE
                     TLQAEVTQESSGCGALTRRSILVRNVTGGSFPYSFSTNTVMDLDMNQFPFRTNLFGGD
                     SFDLVIRSAFGCESITPIGFTPISSNQVMSLGPDREIILGDTAHLGIQSNYTIGSLRW
                     LSQNSLSCTNCLNPVASPLRSSLYILEASDANGCPKRDSLWILVDPDINIFVPNLINT
                     SSTDDRNRLLFIHPPAQVQSVDRFTLFDRWGTLIMSHDQLGPGGQPIEVWDGRFRNED
                     APPAVYVYKLEYTTFDGKQKVKYGDVTLIR"
     gene            complement(6843..7139)
                     /locus_tag="PKM27_17955"
     CDS             complement(6843..7139)
                     /locus_tag="PKM27_17955"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNR09210.1"
                     /translation="MLHITQVIYLHPGPEAEQSFLEFESRVLPLMSNHGGELLLRLRP
                     QKEQKIEGSLEPPFEVHLVSFPSEESLAAYLNDPERLKYLHLKEQAIREAVVYR"
     gene            complement(7489..8613)
                     /locus_tag="PKM27_17960"
     CDS             complement(7489..8613)
                     /locus_tag="PKM27_17960"
                     /inference="COORDINATES: protein motif:HMM:NF024662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4056 domain-containing protein"
                     /protein_id="HNR09211.1"
                     /translation="MPLALPEHKLAAAPPRIIRTCCAFGVNMKMTGLPFVRYNDITGM
                     ERIGPHTYLGSKEEGNGILYTRNGGFVDIGHVRDQADWTAFLYSLIKKYQSEGSRGFT
                     HKLGYEGGPKLLQVSLPQSLKDEDLMVLAGRIAYDLSVWHEIGTWNGTSLIPMLPERY
                     SAFSIEDGYSNMLGILLGIQALKSDLPFEEAMNQVLQESLTRLGALPTLEESRRAMLS
                     VEGDWWSNRIRLPSGKVVKKRQFGIYPCIEPVLLPQYVVGQNGPLVLSLPDLPSQADP
                     NQFYSIYIQLNNKFPVKEIFGDTVRIITQRDFPALIDYAARESEKKYGDLVYKEPRRN
                     RAYGSGQEPEPGISLTGRPKVQAPLGPDGLKGRHGRGGKR"
BASE COUNT         2003 a         2371 c         2281 g         2174 t
ORIGIN      
        1 cggtttcgat atgccaggct ttgcattgct gccgcagggc ggcttcatcc atggtgctga
       61 cgtcgacgcc cgtatattct ttgatggcat cgtgcatgct gagcctccgg taaggccctt
      121 tgaactgaag gaggtgctcg ccgtagggga tatcggtttg cccatggacg gccagggcca
      181 gggtttccag gagttcctcc accatctcca tcatccagta ataatccttg taagccacgt
      241 agatctccat catcgtgaat tccgggttgt gcgtgcggtc catgccttcg ttgcggaaca
      301 ttttcccgat ctcgtagacc ccgtcgaagc cgcctacgat cagccttttg aggtaaagct
      361 cgttggcgat gcggaggtag agaggaatat cgagggtatt gtgatgggtc atgaaaggcc
      421 tggctgccgc ccctccgtgg atggattgca ggatgggggt ttccacttcg agccaacctt
      481 tttcgctgaa atgacggcgc agggcgctga ggagttttga tcttttaaga aagatgtcgc
      541 gtatgcccgg attcaccaaa aggtccacat aacgcatacg gtaacgcagt tccgggtccg
      601 tgaaggcatc gtgcaccttg ccttccgcat ccgttttgac cacgggcaat accttcaggg
      661 atttagaaag cagccggatc tcccggacat ggacggtgat ctcacccatc ttggtgcgga
      721 acacataacc ttcgacgccg ataaaatcac ccaggtcgag ccatttggag aagaggggat
      781 cataggcttc gcccagttcg tcttttttga ggtaaagctg gatcctgccg cgggcgtcca
      841 tgagttccgc aaaggaggct ttacccatgc tccttttcaa cataagccgg ccggccaggc
      901 gcaccgattg gtatctcccg ggatcgtcgt caaaaagcct gaggatgtcg gcggtgaagc
      961 cggtcaccgg aatcaattcc gcgggataag gatctatgcc ggcttccctg atttcagtga
     1021 gagtctcgcg ccgtattagt tcctgttcag ggagtgtcgg tgacatgtgg ttgatttcga
     1081 aggctaaagg tataaaatcg gaaccgaaaa atgatctgtt aaatgaagga tttaacaagg
     1141 aataaaaacg ggattatata tacttttagg ccctgtattc gactgggtca atgaagaaac
     1201 tacatattta tttttttgcc ttcgtggcct ggctgctggg gagtgaaagg gttttggggc
     1261 aggttggatg tccggaaata gtgatggtgc aggtggatgc ctgtaccgcg gatgaaaaat
     1321 taggggaata ctttcagttt gtgacaggag acgtcccact caatttaaat atcagttccc
     1381 tctctattgc aactcatttt acaaggaaca gttggcaatc ggctgcttcg tttatcaata
     1441 ctttgaatgc atctgccaat tgtccaaatg gtccaaaatt tgttgatcca tatgcggctc
     1501 catattttgg aatcattccg gccggtaaaa tggtattggc ctttatcgat gcaaaccccg
     1561 tgatattctc ctcggctgca aggctaagat cactttgtga taaggatgtg atctttgtta
     1621 tcagaggagg tacaagcgat cctctcccaa tgtttgccaa cagatcggaa tgtacagcag
     1681 actgtgaaag gattatcagc ggaatcattg gcaattgcaa ttttgccatc cgatataatc
     1741 catttgaact ttcaaccaac actggggcgt ttatactcgt tgagaataac aatacaggga
     1801 ttgcagcgga atccacggga tgtcagttgt atgcattgaa ttgtgaagcc cctgtcattg
     1861 atgtgcttcc gaatgtggac agtaccgcct gtgcaataga tggatttgag ttgcaaccat
     1921 tcaccgtgga ccccgtcttc agcgccaaat attttacgct cccaaacagg cgtggtgatt
     1981 ccttcaacat tggacaaatc atcactcaat ccattaaact ttatgcgcat gcctatgcgg
     2041 attgcaatcc tgctttaagc gttcaggatt cgattaatat cattataaac caagggcctc
     2101 aaattccttt tcaaagtact caggcttgtg gttttcatgt gctgccccca tttgccggaa
     2161 caaatctttc tcccaatgtt aagtattata cgggtgattt cggaaccggc acccaatacc
     2221 ttccgggtga tattatccgc tccagtgtgg caaccaccat tcgcctgtat gcttatgatg
     2281 tggataccaa agggtgcgca gccgaaggag gatactttat tcagttttta aatcccccaa
     2341 ctattgttaa tcccaaagat accaccgttc catgccgggc aaattatctg ttcccggcca
     2401 ttaccggcac agccttgaca ccactgcgag cttattatac agaaagcatg ggcggaggta
     2461 accgtattgg acaactgaca ccggccacta tgagtgggac ttattttgtc tatgatactt
     2521 tttacaattg cttcgacgaa gacaccttcc gcgtaaccct catccccgtc acccaaatcc
     2581 ccaaagacac catccggaat gatacttttt gtaatcaact cattttacct gatatccaaa
     2641 gcgccagcct catctacaat accgcgagga attttacggg agtcaattcc aatcctgggg
     2701 atattctaaa cgcctccacc aaactatacg tcaaagcgac ggccatgggc tgcgaaacca
     2761 tcgacagcgt gcaatacacc taccgggaac tggacctgga tcccatcacc atcgatgagc
     2821 cctgcaaacc cctgctcgat gccctccccc cgatcaccgg cgccaatctt acagcccaag
     2881 ttgcttattg gaccttgccg ggaggcaaag ggcagcggta cctgccaggg gattccgtgc
     2941 ttgctgcagg ggcgggagga atttatcagt ccaccacgct ttatgccttc gacagcgttt
     3001 catatctctc cggatcctgt acggtggaaa gaaccgtaga gatccccttt cataccattg
     3061 tcgaactcac atccatcagc gacaccgtcc tcgcatgcgg ccagaccctg gaagtacccc
     3121 tggtcaaacc cgatcaggtt taccagggta tggtttatcc tgaccgggcg ctcaccagcg
     3181 ctttaatccc aggcagctca atcccgcaat ccggtactta ttatgtctac gccagttatc
     3241 tgtcctgctc ggacctcgac agtttttccg tgcagttcaa tacagggcct caatatttca
     3301 acaatattga cctcagtgat ccctgcagcg aagtgacgct tctgccggtc aacggtactg
     3361 attttaatcc ggattccact ttttatttca ggggaacctt cggaacgggg gaccgtttca
     3421 gccccgggat gacagtcaac caggctgata ccttttatat tttcgacctg agccagccga
     3481 tgtgcctggt ccaggatacc gtcgtagtga gttttaattc acagcagggc ttcaaccttc
     3541 cggcctcgcc ctgggatgcc tgcgattcca tccggttgcc caatgacccc acggttggca
     3601 acctgcaata tattgtgcaa aacggagatg ccgtcgatat caagtttccc ggtgactgga
     3661 tcacttccag catgcgtgtc gaggcggtcg gcggagatcc aaattgccct aggcgggaaa
     3721 ccatcaccat tcgtattgcc caaaccccgg aactggtccc catgcgggac accctgctct
     3781 gtgatccctt gcgtttgcct gtcctgacgg gtaagaacct ggcggacccc cattatcata
     3841 ccttgcccaa tgacggcggc gaatccctgt acgccgggga taccgtattc caaaccggca
     3901 cctattatcc cttcagccgg aacggggctt gtgtggatga ggacaccgta caaatcacca
     3961 ttgtcaatag gcccaggata aataagttaa ccgatcagag ggtttgtgaa aaatatatcc
     4021 tgcccctgcc tgccggagcc aatttggatt ccctttatta ttatgacgat cgctccaggc
     4081 agggagagcg ttatgccatc ggcgacaccc tccgccgaag catgaaaata tatgcttacc
     4141 aggatgtgcc attatgccca gctattgatt ctatggtcat caccgtgtta gacagcacga
     4201 ccacggaatt catgctctcc gataccttgt tttgtgtggg cacacccctt ttggccaacc
     4261 ataccggcga ccgcggccct gaaactactt ttacctgggc tttttccgga tccacccagg
     4321 tgacctttaa aggaggtaat gcggaccaac gctgggacct cggcccgggg aattacaaca
     4381 tcaccctttc cggggaaacc ggcagttgtg atggcccgcc caccacacac agcgtccgca
     4441 tccacgatac tcttgcagcc ctcaccgggc tcaattgcac ccgcggaccg gacaatattg
     4501 ccttccgctg gaaccaggtg cccggagccc ttgaatacgg gatcgagttg ctggccggac
     4561 cgacaggggt aagaactccc tttgagatgt tgttttccaa cctggcgccc ggccaaccgg
     4621 tgggtatccg ggtgacccct ttgggaggca ttccctgtgc caatggccag gcggattcgc
     4681 tggtctgctt tcctacggtt ttatgtgagc ctgtgaccgt agaaataacc caggaaggcc
     4741 cattttgttc cggcgatggt ccgatcgaac cggtggcatt gatccggggc gcttcagacc
     4801 cggcttccta cacacggaca tggtccgggc cgggaatggt ccgggatact ttttatccgg
     4861 cccaggcggg tcccggagaa catatcatca cctttaccct cacccgggac agttgcatct
     4921 ttaccgacac cgtggtattc cgggtcggcg gtggttcggt acagatcctc aatgaccagg
     4981 gcaccatctg caatcctgcg gatcaggacc agttcaacat ccggatgttc atagacgcat
     5041 tcgacagacc ctatgatgtc cactatactt ataatgggcg gccttccgct tttccttccg
     5101 ggtccgacac ttttaccctt tcggtaaggt ccggtgtgat ccggatcgac agcgtggtgg
     5161 atgcttcggg ttgcaagctg cctataaaca gcatggctgg aaccgacagt ttccgcctgg
     5221 tgcaacccct ggcccccgtg gacacccagt ttatctgtga ttttggccga agggcctggt
     5281 cttaccaggt ggtgatcaac aaccccaatc ctgctgaacc gatgctcgtg ctttcagggg
     5341 gtggaacgtt ccaggactcg ctgtacacct ctccgccggt gcctttcggg caaaggcatc
     5401 tggccaggat cagccgggat gcggcctgtg ataccctggt tttcgacctg actttcagtt
     5461 gcgactgcac cccgcaccgg gataccgtgc ctaccgcggt ccaatgcgaa ggggataccg
     5521 tagtcatcgg caaccggacg tacacggccg gcaacccctt tcatatcgat accatcccgc
     5581 ctgcggtgat tggagcctgc gacaccttcc gggtcgtgaa tatcactttt ttgccccgcg
     5641 gacgccaggt gctcaatccc gtgttttgcc cgggggatac ccttagagtg ggcaatgtag
     5701 tttttggtca tggcaaccca ataggcgaag tgtacctgcc gggtatggcg gccaatggct
     5761 gcgacagctt ggtggtcgtg caggggctgg cattgaaccc ggtgacggtc atcatcaggg
     5821 atacgatctg cgccggcgat accctccggc tttcgggcat tgaatttcat aacggccacc
     5881 gggcggattc ggtgcgcctc gcgggcgggg ccgccaacgg atgtgattcc gtggtttatt
     5941 ttttcattca tgtggaaacg ctccaggccg aggtgaccca ggagtcttcg ggttgcgggg
     6001 cattgaccag gcggtccatc ctggtgcgca acgtcacagg cggttcattt ccctattcct
     6061 tcagcaccaa taccgtgatg gacctggaca tgaaccaatt cccgttccgc accaacctct
     6121 tcggcgggga cagctttgac ctggtcatcc ggtccgcatt cggttgtgaa agcatcactc
     6181 ccattggttt tactcccatc agttccaacc aggtgatgag cctggggccc gaccgggaga
     6241 tcatcctcgg cgacacagcc catttgggca ttcagagcaa ttataccatc ggttccctgc
     6301 gatggctcag ccaaaacagc ctttcctgca ccaattgcct caatccggtg gcctcgcccc
     6361 tgcgttccag cctttatatc ctggaagctt cggatgccaa cggttgcccc aaaagggatt
     6421 ccctgtggat cctggtcgac ccggacatca atattttcgt acccaacctg atcaatactt
     6481 caagtacgga tgaccgcaac cggcttcttt ttatccatcc cccggcccag gttcagtcgg
     6541 tggaccgctt caccctcttt gaccgctggg gcaccctgat catgagccat gaccagctcg
     6601 gccccggtgg ccagccgatc gaggtatggg acgggcgttt ccgcaacgag gatgcccctc
     6661 ccgccgtcta tgtgtataag ctggaataca cgacgtttga cggaaaacaa aaagtgaagt
     6721 atggggatgt gacgttgatc aggtgagagg agtgaggatt gaggggtgag gtttgaggat
     6781 tgaggattga ggtacgggtg attactgagt attgttgtca ctcaccaccc accacccacc
     6841 acctacctgt acaccaccgc ttccctgatc gcctgttcct tcagatgcag atatttcaaa
     6901 cgttcggggt cattgaggta ggcggccagg gattcttcgg aaggaaaaga taccaggtgg
     6961 acttcaaagg gcggctccag gctcccttct attttttgtt ctttttgggg ccttagcctc
     7021 agcaacaatt cacctccatg gttcgacatc aatgggagta ccctggattc aaactcgagg
     7081 aaggattgtt cggcctcagg gccgggatga agatagatga cctgagtgat atgcaacatg
     7141 gtatttggga tatgaagctg caaaggtcgt aaagagtgca aagacccggc aatggaatga
     7201 tgggttattt tctattatag attcaaacat cgctcgcagc ggtgaatcaa gatgatgcac
     7261 cagtatttag cgcaaaggcg caaaggcgca aagaagctgt aatggaaggg aggtaatgta
     7321 tttatacgga ttcccactgc actctcaacg cccacagtga taactaaaga aatgatgcac
     7381 cgcaatgatg cccaaggact caaattccga tatcagccga gtgagtactt acaaacttgc
     7441 caacttgcta actgactaac tgactaactg actaactgac taactacatc accttttccc
     7501 accccttcca tgcctccctt tcaacccgtc cgggcccagg ggcgcctgta ctttcggccg
     7561 gccggtcagg gaaatgccgg gctctggttc ttggcccgag ccataagccc tgtttctcct
     7621 cggttccttg taaacgagat ctccgtattt cttttcgctt tcgcgggccg cgtaatcgat
     7681 gagggccgga aaatcgcgct gggtgatgat gcggaccgta tctccaaaaa tttctttgac
     7741 cgggaattta ttattcagtt ggatataaat ggaataaaac tggtttggat cggcctggga
     7801 gggcaggtcc ggcaggctca gcaccagggg gccgttttgg ccaacaacat actgcgggag
     7861 gagaacgggt tcaatgcagg gatagattcc gaattgtctt tttttgacca ctttaccgct
     7921 agggagccgg atccggtttg accaccagtc cccttccaca gagagcatgg cccgcctgga
     7981 ctcttccagg gtaggcagtg cgcccagcct ggtcaggctt tcctggagga cctggttcat
     8041 ggcctcttca aagggaaggt cgcttttaag ggcctgtata ccgagcagga tacccagcat
     8101 attggaataa ccatcctcaa tggaaaaggc ggaataccgt tccgggagca tggggatgag
     8161 ggaggtgccg ttccaggtgc cgatttcatg ccatacggaa agatcatagg cgattcgccc
     8221 ggccaggacc atcaggtctt cgtctttcag ggattggggt agggatacct ggagcagttt
     8281 ggggcctcct tcgtaaccga gcttatgcgt aaatcccctg ctgccttcgg actggtattt
     8341 ttttatcagg ctgtacagga aggccgtcca gtccgcctgg tcccggacat ggccaatatc
     8401 cacaaatcct ccgttgcggg tgtacaggat cccgttgcct tcctccttgc tccccagata
     8461 agtatggggc ccgatccgtt ccatcccggt gatgtcgttg taccgcacaa aaggcaaacc
     8521 ggtcatcttc atattgaccc caaaagcgca acaggtgcga atgatgcggg gcggggccgc
     8581 ggccagtttg tgttcaggca gcgctaaagg catggatcgt acaggagtca gaatccagat
     8641 ggaaataata atgaggaaca gccttgtctg catgaaaaag aaggcaaaaa tagtaataaa
     8701 tgaatgaaag tcagtggggt agattcttaa tgggaggtta atttagttag taagttggca
     8761 agttagctag ttagctagtg ccgcgccaac tattattgcc taaaaacagc taatggtttt
     8821 cggttgctg
//
LOCUS       DAOQXT010000153         8793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_8449_length_8793_cov_7.002060, whole genome shotgun sequence.
ACCESSION   DAOQXT010000153 DAOQXT010000000
VERSION     DAOQXT010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8449_length_8793_cov_7.002060"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /gene="kdsB"
                     /locus_tag="PKM27_17965"
     CDS             complement(<1..627)
                     /gene="kdsB"
                     /locus_tag="PKM27_17965"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNR09212.1"
                     /translation="MKIIGVIPARYGSTRFPGKPLALIRGKSMIEHVYRGCGQSTKLS
                     QLVVATDDRRIADHVLDFGGKAIMTSSGHVSGTSRCEEACRLLGGADWCINIQGDEPL
                     IRGKLLDDFIGKFAHFPAEAVLTLVRQLREEHLIYSPNTVKCVRDRNDKALYFSRSVI
                     PHPRSQTTPAYYQHIGIYAYPIPVLKAITGLEPTPLEQAESLEQLRWME"
     gene            704..1837
                     /locus_tag="PKM27_17970"
     CDS             704..1837
                     /locus_tag="PKM27_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNR09213.1"
                     /translation="MLRSFFIGLLVTKMAGFPIQVKANDSWIKDTIYVKLDDTYGKYV
                     EHRVEKKQTLFSLAKAYGIDLYDVYDYNPALKTRILAVNDLLHIPISSDHIITNESQL
                     DSNKKYRPVYYIIKPKDNLYRISKFYFNMAFDDVVTRNRLTSHTIRVGQKLMIGWFDP
                     SKKESGNPDYKPNIIKNEQITLEQKWTEANPSAFTVEYKAIDLTSSEWASTYGKKENS
                     SDRLIEIEKPIAEISLKERKTEAATSPEAPALKTAEASKLTGAGHEASLSNHETVKSA
                     TKTKVLKRRMVTQNGVAQWAKSGNQVQDLYVLHPTAPVNSVVELTNPMTHRKIYAKVL
                     SHMPPKLYPDDVSVVVSPGVANLLGAIDSRFYVKLRFVEETIQ"
     gene            1899..3263
                     /locus_tag="PKM27_17975"
     CDS             1899..3263
                     /locus_tag="PKM27_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR09214.1"
                     /translation="MKYAENILMTIGNTPLVRLHKVCNELEARVFAKVETFNPGHSIK
                     DRMALKMIDDAEAAKLLSPGGTIIECTSGNTGMGLALVAAVRGYKCIFTTTDKQSAEK
                     INLLKAFGAEVIVCPTNVESSDPRSYYSVAERLSKQIPNSFWCNQYDNLSNTQAHFET
                     TGPEIWDQTDGKITHLIAGVGTGGTISGVGRYLKSKNPGIKIWGVDTYGSALKKYHET
                     GILDPKEIYPYITEGIGEDIIPKNVDFSVIDHFEKVTDKDAALAARRLAREEGLLMGY
                     SAGSALAGLLQMKDSLTKNDVVVIIFHDHGSRYVGKIYNDDWMRERGFLDSDLKVKDL
                     VRAKTDGQFFFVTCSAKVRDALHLMKANDISQLPVSRDDQFIGAVTETRVLSYILENP
                     VSHLEDNIEQIMGDPLPHVPFDMSIQDVSRYFDRKIPAVITRDTAGNWHVITQHDIIR
                     SIRG"
     gene            3287..4345
                     /locus_tag="PKM27_17980"
     CDS             3287..4345
                     /locus_tag="PKM27_17980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNR09215.1"
                     /translation="MALFKRIARYGILLLILVHFKVNAQDRHFSQFHASPLQLNPALT
                     GMINGTFRVNMNYRNQWNKVIGNNFQTIAVSGDMNIKSPIKSQGKDLVGLGLSIFSDK
                     VAGYDLNHTELAATGAYHKSTGAGQFLSLGLKMGLSQRSLNYENLRFEDQYVEGQGYT
                     NTTLENLPENNLSMMELALGLQYTLSGARDQGLNIGLSLHHITSPDFSFFNLNPPDPI
                     APPKKPLYGLLSFHAGGSLALSPTQSLLPRLWFLSQGPHYLFNIGTLLRTPLNQDNTS
                     ALHAGGFVRIVNGKKGPSPESFIPMIGFELGNVNIGLSYDANLRRLAPSYFQQAIFEL
                     SLSILGSHDNEGFFCPKL"
     gene            4355..5140
                     /locus_tag="PKM27_17985"
     CDS             4355..5140
                     /locus_tag="PKM27_17985"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNR09216.1"
                     /translation="MKIGISLSGGGAKGIAHVGVLQALEDQGIEVDIIAGTSAGAIVG
                     ALYSSGMSPADMMSAIRNTSLLRIFGFQWPDRGLGEMTYLRRLLARQIGIDSFSALKK
                     PLYVTLTNIVTGEVEIKCSGDLFDVVCASSSVPLLFKPVEINGHYYLDGGVIMNMPVR
                     CLIGKCDLILGVNLVNHQQVAEKYLSSWREILSRVFDLSVFNNVKPEMELCDIVIEPT
                     DIHKYSRFNFNQAEPLYKAGYEGAMLQMPAIKALVQEGQRSSE"
     gene            5160..5489
                     /locus_tag="PKM27_17990"
     CDS             5160..5489
                     /locus_tag="PKM27_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09217.1"
                     /translation="MENRLVVVAYKPKPVKESELENLVQGHHARLLEEGLVTAKPPFI
                     GKAKDGTLLEIFEWISPEAIQAAHTNPAVLTLWNEFEAVCEYLPAGQVPELQQMFSEF
                     SAVKLEL"
     gene            complement(5537..6865)
                     /locus_tag="PKM27_17995"
     CDS             complement(5537..6865)
                     /locus_tag="PKM27_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNR09218.1"
                     /translation="MKGGMVLLFLVPVLIHAQNRPLILPERDRARVINEVLAHRFEKV
                     LPGLMRREGIDLWILISREYHEDPVMKTMLPAEWISARRRTVFVFFDKGADTPSVEQL
                     AIARYDVGLLKASWNLNVYPDQWDALMEIIRVRQPKKIGINFSPRYAHADGLSHFEYD
                     QFISRLPGEFKPRVVSAETLAVAWLETRSSMEMTLYEHIGRIAHQILREGLSDQVIQP
                     GITTTEDLVWWYRQKVTELGLETWFHPTVDVQRPDTGRFDHLRTFSKRPGPELIQPGD
                     LLHVDFGISYLRLNTDMQEHAYVLKPDEREVPGYLQEAFAKAHRLQDILTAHFKTGRT
                     GNEILATALAQAKGEGIEGAIYTHPIGSHGHAAGPTIGMWDNQGRVTGPGDYPMFPNT
                     AYSIELNASVTLPEWGGKTIRIMLEQDGFFDGKTFRYIDGRQEEIYPIQK"
     gene            7136..7669
                     /locus_tag="PKM27_18000"
     CDS             7136..7669
                     /locus_tag="PKM27_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNR09219.1"
                     /translation="MRKIIYYVAVSLDGFIAGPNDDISGFTAQGNGVDQYFADLKAFD
                     TVIMGRNTYEFGYKYGLIPGQPAYPHMDHYIFSNHLQFEKQHRKVKIRKPDLEEIIRL
                     KESNGTDIYLCGGGQFAGWLLDHQQIDLLKIKLNPFIQGEGVPLFGKSCKNYKARLLD
                     SAPYEHGLQIMTYEILY"
     gene            7796..8539
                     /gene="bla"
                     /locus_tag="PKM27_18005"
     CDS             7796..8539
                     /gene="bla"
                     /locus_tag="PKM27_18005"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HNR09220.1"
                     /translation="MNLNIFSILTVAFYFASCAPALRQRAFTGSHLRILPLSPRTYVH
                     LSNLETETFGRVSCNGLVYIDRHEALILDCPVNDTAARELIRWIEEDTRSRITGVVIN
                     HFHNDCLGTLDLFHEKGIESWANETTLLLARKTGVPENKIPRNAFNQSLSLQNGKGRV
                     INAFFGEGHTRDNIVSYIPGERVLFGGCLVKALQASKGYLGDADTAAWSGTVSKVKAA
                     YSEVRYVVPGHGDYGDRSLLDYTMSLFQQ"
BASE COUNT         2223 a         2385 c         2099 g         2086 t
ORIGIN      
        1 ttccatccat cgaagttgtt caaggctttc cgcctgttca aggggcgtgg gttccaggcc
       61 ggtgatggct ttcaaaacag ggatggggta agcataaatg ccaatatgct ggtaataggc
      121 cggggtggtt tgactccgcg gatgaggaat tacgctccgg ctgaaataca gggctttatc
      181 gttgcggtca cggacacatt ttacggtatt ggggctgtag atcagatgct cctctctgag
      241 ctgccgtacc agggtaagta cagcctctgc cggaaagtgc gcaaattttc caataaaatc
      301 atcgagcagt tttccccgga tcagcggttc atcaccctgg atgttgatgc accagtccgc
      361 accacccaga aggcggcatg cttcttcgca acgcgaggtg ccgctgacat gaccggagct
      421 cgtcataatc gcctttcccc caaaatccag gacatggtct gcaatgcgcc ggtcgtcggt
      481 ggctaccaca agttgggaca attttgtact ttggccgcaa cccctgtata catgttcgat
      541 catcgatttg cccctgatca gggcaagcgg ttttcccggg aaccgggtcg acccataacg
      601 ggcgggtatg accccgataa tcttcatgtt aaaaccgtta aaacgttaac taacatatta
      661 atattaattt taatactttt gccattcata ttaaagtgat tatatgctga ggtcgttttt
      721 tatcggattg cttgtgacca aaatggctgg atttccgatc caggttaaag ctaatgattc
      781 ctggataaaa gataccatct atgtaaagct ggatgatact tatggcaagt acgtggaaca
      841 ccgggtggaa aaaaaacaaa cccttttttc cctggccaaa gcctatggca tcgacctgta
      901 tgacgtgtat gattacaacc ctgctttaaa aacccggatc ctggccgtga atgacctcct
      961 ccacatcccc atatccagcg atcacatcat cactaatgaa tctcaacttg attccaacaa
     1021 aaaataccgg ccggtatact atatcatcaa acccaaagac aatctctacc ggatttccaa
     1081 attctacttt aatatggcct ttgacgatgt ggtcacccgc aaccggctca cttcccacac
     1141 catccgggtg ggtcaaaaac tgatgatcgg atggtttgat ccttcgaaaa aggaatcggg
     1201 aaatccggat tacaaaccga atattattaa aaacgaacag atcaccctgg aacaaaaatg
     1261 gacggaggcc aatcccagtg cattcaccgt ggaatataag gccatcgacc tcacctcctc
     1321 cgaatgggcc agtacttacg ggaaaaagga aaattcctcc gaccggctga tcgaaattga
     1381 aaagcccatt gcggagatca gcctcaaaga gcgaaaaacc gaagcggcaa ccagtcccga
     1441 ggcccccgcc cttaaaaccg cagaggcttc caaattgacg ggagccggcc acgaagcaag
     1501 cctgagcaac cacgaaaccg tgaaatccgc tactaagacc aaagtgttga aacgcaggat
     1561 ggtgacccaa aacggggtcg cacagtgggc gaagtcgggt aaccaggtcc aggacttgta
     1621 cgtattgcat cccaccgccc cggtcaattc ggtggtggaa ttgaccaacc ccatgaccca
     1681 ccggaagatt tatgccaaag tgctgtccca tatgcctccc aaactttatc ccgacgacgt
     1741 cagcgtggtg gtctccccgg gtgttgcgaa cctcctggga gccatcgaca gccgctttta
     1801 tgttaagctc cggtttgtgg aagaaaccat ccagtaaatt tgttgtccgg attggggttt
     1861 gtttctcatg gtattcccct ccctgaattc agaaagaaat gaaatacgct gaaaatatac
     1921 tcatgaccat cgggaacacc cccctggtcc ggctccataa agtatgcaac gaactggaag
     1981 cccgggtttt cgccaaggtg gaaaccttta atcccgggca ttcgatcaag gaccggatgg
     2041 ccctgaaaat gattgacgat gcggaagctg cgaaattatt gtctccggga ggcaccatca
     2101 tcgaatgcac ttccgggaat accggcatgg gacttgcgct cgtggccgca gtgcgcggat
     2161 acaaatgcat attcaccacc accgataaac aatcggctga aaaaataaat ctcctgaaag
     2221 cctttggggc tgaagtcatc gtctgtccca ccaatgtgga atcgtccgat ccaaggagtt
     2281 attattccgt ggccgaaaga ctgtccaaac aaattcccaa ttccttctgg tgcaatcaat
     2341 acgacaacct ttccaacacc caggctcact ttgaaaccac aggacccgag atctgggacc
     2401 agaccgatgg aaagatcacc cacctcattg ccggagtggg caccgggggc accatttccg
     2461 gcgtaggccg ctatcttaaa tccaaaaatc ccgggatcaa aatctggggt gtggatacct
     2521 atggttcggc ccttaaaaaa tatcacgaaa ccggcatcct cgaccctaag gagatctatc
     2581 cctatatcac ggaaggaatc ggagaagaca tcattcctaa aaatgtggat ttttccgtca
     2641 tcgaccattt tgaaaaagta acggataagg acgccgccct cgccgcgcgc aggctggccc
     2701 gcgaggaggg gctgctgatg ggctattcag ccggctcggc cctggccgga ttgttgcaaa
     2761 tgaaggattc gttgaccaaa aacgatgtgg tggtcatcat ctttcacgat cacggttccc
     2821 gctatgtggg caagatctac aacgacgact ggatgcgcga gcggggattc ctggattcgg
     2881 accttaaagt caaggacctg gtccgtgcga agaccgacgg tcaatttttc ttcgtcacct
     2941 gctccgccaa ggtgagggat gccctccacc tgatgaaagc caacgacatt tcacaactcc
     3001 cggtatcccg ggacgatcag ttcatcgggg cggttaccga aaccagggtc ttgtcctata
     3061 tcctggaaaa cccggtaagc cacctcgaag acaatattga gcagatcatg ggcgatccgc
     3121 tgccccatgt tccttttgac atgagcatcc aggatgtcag ccgatatttt gaccggaaaa
     3181 tccctgcggt gattacccgg gacaccgcgg gcaactggca tgtcatcacc cagcacgata
     3241 tcatccgttc gatccgtgga taaaaaaagg acttacattt gtaagaatgg ccctgtttaa
     3301 gcggattgca cgatacggga ttttattact catattggtt cattttaagg tgaatgcgca
     3361 agaccgccat ttttcccagt ttcacgcatc ccccctccag ctcaaccccg ccctcaccgg
     3421 catgatcaac gggaccttcc gggtcaatat gaattaccgc aaccaatgga ataaggtgat
     3481 tggcaacaat ttccaaacga tcgccgtctc gggggatatg aatatcaaat cccccattaa
     3541 atcccagggg aaagatctgg taggcctcgg cctctccatt ttttccgata aagtcgcggg
     3601 ctacgacctg aatcacacgg aattggccgc tacgggggcc tatcataaat ccaccggtgc
     3661 cggccagttt ttgagcctgg gcctcaaaat gggactttcc cagcgcagtc tcaattatga
     3721 aaacctgagg tttgaagacc agtatgtgga aggccagggt tataccaaca ccaccctgga
     3781 aaacctcccg gaaaataatt tatccatgat ggagctcgcc ctgggccttc aatatacctt
     3841 gtcgggtgcc cgggaccagg gattgaacat aggccttagc ctccaccaca tcacttcgcc
     3901 cgacttttca tttttcaacc tcaaccctcc cgacccgatc gccccgccaa aaaaaccctt
     3961 atacggcctg ttatcatttc atgccggcgg gtccctggcc ttatcaccga cccagtccct
     4021 gctgccccgc ctgtggttcc tcagccaagg cccccattac ctttttaata ttggtaccct
     4081 gctccggact cctttgaacc aggacaacac cagcgccctc cacgccggcg gattcgtgcg
     4141 catcgtcaac ggtaaaaaag ggccctcccc cgaatccttt atcccaatga tcggctttga
     4201 actgggcaat gtcaatatcg ggctcagtta tgacgccaac cttcgccggc tggccccttc
     4261 gtatttccaa caggccattt tcgaattgtc cctctccatc ctcggcagcc atgacaacga
     4321 aggatttttt tgtcctaaac tttaattccc aatcatgaaa atcggcatct cactgagtgg
     4381 aggcggggcc aagggaatag cccatgttgg ggtcctgcaa gccctggaag accaggggat
     4441 cgaagtggat atcattgccg gtaccagtgc gggggccatc gtcggcgctc tgtattcctc
     4501 cggcatgtcg ccggccgaca tgatgtcagc catccgcaat acgagcctgc tgcgcatatt
     4561 cggttttcaa tggccggacc ggggcctggg ggagatgacc tatctccggc gcctgctggc
     4621 ccggcagatc ggcatagact ctttcagcgc attaaaaaaa ccgctttatg tgacgctcac
     4681 caatattgta accggggaag tggaaatcaa atgttcgggt gacttgtttg atgtggtttg
     4741 cgcttcgagt tcggtacccc tgctgtttaa accggtggag atcaacggtc attattacct
     4801 cgacggagga gtcatcatga acatgccggt ccggtgcctg atcggaaaat gtgatctgat
     4861 cctcggggtc aacctggtca accatcaaca ggtggccgaa aaatacctga gcagctggag
     4921 agaaatttta tcacgggtgt ttgacctgtc cgttttcaac aatgtcaaac cggaaatgga
     4981 gctctgtgac atcgtgatcg aacctacgga tattcataaa tacagccggt ttaatttcaa
     5041 ccaggccgag ccgctgtata aagccggtta tgagggggcc atgcttcaaa tgcccgcgat
     5101 aaaagccctg gtccaggaag gccagcgctc ctcggaataa ttttctaaac ccgaatccaa
     5161 tggaaaacag attggtcgtc gttgcctaca aaccaaagcc cgtaaaagaa tccgaacttg
     5221 aaaatctggt ccagggacac catgcccggc ttcttgaaga aggcctggtc accgccaagc
     5281 ctcccttcat cggaaaagcg aaagacggta ccctactgga aatttttgaa tggatttctc
     5341 cggaggccat ccaggccgcc cataccaacc ctgccgtcct gaccctgtgg aatgaatttg
     5401 aagccgtctg cgagtatctc ccggccggcc aggtcccgga attgcagcag atgttttcgg
     5461 aattctctgc agtgaaactg gagctttgaa ccttcactcc cttcgccttt cgcccttaac
     5521 ccttcaccct acccgcctac ttctgtatcg gataaatctc ttcctgcctc ccatcgatat
     5581 acctgaaggt tttaccgtca aaaaatccat cctgctccag catgatccgt attgttttac
     5641 cgccccattc cggaagggta accgaagcgt ttaattctat ggaataagcg gtgttgggga
     5701 acatcgggta atccccaggt cctgtcaccc tgccctggtt gtcccacatc ccgatggtgg
     5761 gtccggccgc gtggccgtgc gatcctatgg gatgggtata aatggccccc tcgattcctt
     5821 ctccttttgc ctgggccagg gcggtcgcca gaatttcatt tccggtcctc ccggttttaa
     5881 aatgcgcggt gagaatatcc tgcaaacggt gcgctttggc gaaagcctcc tggagataac
     5941 cgggcacctc cctttcgtcc ggtttaagca cataagcatg ttcctgcata tcggtgttga
     6001 gcctgagata acttattcca aaatcaacat gcagcaggtc tccgggctga atgagctcag
     6061 gccccggcct tttggaaaaa gtacgcaaat ggtcaaagcg ccccgtatct ggcctctgga
     6121 catcgacggt aggatgaaac caggtttcca gtcccagttc cgtcactttt tgccggtacc
     6181 accagaccag gtcttccgta gtcgtgatcc ccggctgaat cacctggtcg cttagtcctt
     6241 ccctcaggat ctgatgcgca atgcgcccaa tatgttcata gagagtcatc tccatggacg
     6301 agcgcgtttc cagccaggct acggccaaag tttctgcgga aaccaccctc ggtttgaatt
     6361 caccgggcag acggcttata aactgatcat attcaaaatg gctcaggccg tcggcgtgag
     6421 catacctcgg cgaaaaatta atccctattt ttttaggctg gcggacacgg atgatttcca
     6481 tgagcgcatc ccattggtcc ggatatacat taaggttcca ggatgcttta aggaggccaa
     6541 catcataacg ggcgatggcc agctgttcca cggagggagt atccgctcct ttgtcaaaaa
     6601 atacaaaaac cgttcttcgg cgggcggaaa tccattcagc gggaagcatg gttttcatga
     6661 ccgggtcttc atgatactcc cgcgaaatca ggatccacag atcgatgcct tcccgacgca
     6721 tcaggcccgg caatactttt tcaaaccggt gcgcaagcac ctcgttgatc accctggccc
     6781 tgtctctttc aggcaagatc agaggccggt tttgcgcatg gattaaaacc gggaccagaa
     6841 acaataagac cattcctcct ttcattgggc aaatatagtt ggagggaatt gcaagtcgca
     6901 tccgggagaa cagggggtgt atgacaaaat gcagttttac attttctgga taaacgcgac
     6961 ctggaaggtt ttacttcgtt tttatgcgag ttgaaacctt tcaggtcttg ttatcaagca
     7021 aaaagacctg cattgtaaca tgcacccaga acagggggac ctcgacgtat ttctataaat
     7081 ttcgcgaaat taacccttca aaaaaccgga tcttcatagt cccccaaacg gaaccatgag
     7141 aaaaataatc tattacgtag cggtatccct ggacgggttc atcgcagggc ccaacgatga
     7201 catttcggga ttcaccgccc agggcaacgg tgtggaccaa tattttgcgg acttaaaagc
     7261 atttgacacc gtgatcatgg gaaggaacac ctatgaattt ggatataaat acgggcttat
     7321 cccgggccag cccgcatatc cccacatgga tcactacatt ttttccaatc accttcaatt
     7381 tgaaaaacaa caccggaaag tcaagatacg caaacccgac ctggaagaaa tcatccggct
     7441 gaaagagtcc aatggaacgg acatctattt atgcggcggc ggccagtttg cgggatggtt
     7501 gctggaccat cagcaaatcg acctgctcaa aatcaaactc aaccccttca tccagggtga
     7561 aggcgtgccc ctttttggaa aatcgtgtaa aaattataaa gcccgcctgc tggattcagc
     7621 tccctacgag catggcctgc aaatcatgac ctatgaaata ttgtattgag gaatggtcga
     7681 attgtggaat tgtggaattg tagaattgtt tagtgatttc tggatccagg gtatgccctt
     7741 ttctcctttg cttcgcggag catctttttc aacttcagtc tcaaattttg ctcaaatgaa
     7801 cctgaatatc ttctccatct tgacggtcgc tttttacttc gcaagctgtg ccccggccct
     7861 tagacaaagg gctttcacgg gaagccacct ccggatcctg cccctcagtc ccaggaccta
     7921 tgtccacctg tccaacctcg aaaccgaaac ctttggcagg gtctcctgca acgggctggt
     7981 ctatatcgac cggcacgaag ccctgatcct ggactgtccg gtcaacgata ccgccgcgcg
     8041 ggaactgatc cggtggatag aggaggatac ccggtcccgg atcaccggag tggtcatcaa
     8101 ccattttcac aacgattgtc ttggcaccct ggatttgttt catgaaaaag gcattgaatc
     8161 ctgggccaat gaaaccaccc tcttactggc caggaagacc ggtgttccgg agaataaaat
     8221 ccccagaaat gccttcaacc aatcccttag cctccaaaac ggcaaaggcc gggtgatcaa
     8281 tgcctttttc ggtgaaggcc atacccgcga caatattgtg agttatatcc ccggagagcg
     8341 ggtccttttc ggcggctgcc tggtcaaagc cctgcaggcc tccaaaggct atctcggaga
     8401 tgcggatacg gcagcctggt ccgggactgt ttcgaaagtg aaagccgcct attctgaagt
     8461 cagatatgtg gtccccggcc acggagatta tggtgaccga agcctcctgg attacacgat
     8521 gagtctgttt cagcagtgag ttttgagcga tcagctttca gcgatcagct atgagcaatc
     8581 agcttggaac tgacccctga ctactgacta actgaccact agctaactga caaactgcct
     8641 atgctaaacc ctgaatcccc tatctttgtt acacctctat gttgaattgg ttttttggta
     8701 aaaaggaacc ggagatggaa ggggacagcc cttttgacaa actcttcaaa ttatttaagg
     8761 agctgctcct taataccagc ggggacgtca acg
//
LOCUS       DAOQXT010000154         8481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_8829_length_8481_cov_7.703537, whole genome shotgun sequence.
ACCESSION   DAOQXT010000154 DAOQXT010000000
VERSION     DAOQXT010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_8481_cov_7.703537"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1013
                     /locus_tag="PKM27_18010"
     CDS             90..1013
                     /locus_tag="PKM27_18010"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HNR09221.1"
                     /translation="MKQTFRGNGKLLITGEYAVLDGALALAVPTKAGQTMNVRDGRGA
                     EINWKAYDSTGKVWFEAHFSLYDFSPLKTNDPEKAKKISKLLKECTRQNSEFLSAWKG
                     QTVETYLDFPLDWGLGSSSTLIYCLSQWADANPYYLLFNTCGGSGYDIACAYAEGPVF
                     YQMGEENIRIDEVYFNPPFKDSLYFLHLGKKQDSSAEVKEYKIKSKPSQEILDKISAI
                     SEEMVASKDSKQFQKLMASHEDLVSGLLDRPPIKKQLFPDFKGAIKSLGAWGGDFALV
                     CSDEPASSVRSYFESAGYPTLIPFTELVLNE"
     gene            1021..1761
                     /locus_tag="PKM27_18015"
     CDS             1021..1761
                     /locus_tag="PKM27_18015"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNR09222.1"
                     /translation="MRAHWYTGIVTGIHELAPQVRSFTLEIPEVDRFEFKAGQFVTFD
                     LPVSEKRINRWRSYSIASPPDQGNKIELCIVHFEGGIGSSYFFNEINLGSELKFKGPE
                     GGFILPEVLDHDLVMICTGTGIAPFRSMLLDIKQRGVGHQGLHLIFGTRKQEDILYRE
                     EWSSLERELPGFRFDIALSREFPGPDWASHMHQGYVHPVYQKAYTPYRPDVHFYLCGW
                     TKMIDEAIANLFAGMRYPREQIHYELYG"
     gene            complement(1818..3722)
                     /locus_tag="PKM27_18020"
     CDS             complement(1818..3722)
                     /locus_tag="PKM27_18020"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNR09223.1"
                     /translation="MAEVKYTEDDIKSLDWKEHIRLRPGMYIGKLGDGAAPDDGIYVL
                     IKEVIDNCIDEFTMGYGKQVDITCSGGSVEIRDYGRGVPLGKVVECVSRINTGAKYDS
                     KAFKKSVGLNGVGTKAVNALSDLFFIQSFRDGKSKLAEFRKGVIVKDPPLSGTSEENG
                     TLVRFTPDASIFKHYEYHLPHIENQLWNYAYLNAGLKIRFNNKVMVSKNGLLDLLGRK
                     TEGEELRYPIIHLKGEDIEIAMTHGNEYGEQYYSFVNGQNTTQGGTHLAAFREAIVET
                     VRNHFKKEYDPKDIRASIIAAVSVRIEEPVFESQTKTKLGSQAIAPEGQSLKSFVSEF
                     ITSQLDNYLHKHPDAASALQARIQQSERERKEIAGVRKLANERAKKANIFNKKLHDCR
                     VHFSDKADEELRLATTLFITEGDSASGSLTKARNVQTQAVFSLRGKPLNCYGMSKKIV
                     YENEELNLLQHALDIEDGIENLRFNQVVISTDADVDGMHIRLLLLTFFLQFFPDLVRN
                     GHLFILETPLFRVRDKQQTFYCYSIREKEEAIAALRGKPEITRFKGLGEISPNEFGRF
                     IGNDMRLEPVMLQEGTAIEHILEYYMGKNTPDRQEFIIENLRTGLDELRPEEVMVNEE
                     AVEAVETLEG"
     gene            3876..5765
                     /gene="acs"
                     /locus_tag="PKM27_18025"
     CDS             3876..5765
                     /gene="acs"
                     /locus_tag="PKM27_18025"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNR09224.1"
                     /translation="MKITSLEEYQKTYKRSIEDPEGFWAEIASQFTWKKKWNKVLDWN
                     FTEPRVKWFEGGQLNITENCLDRHLADKPDRTALIWEPNDPAESTRRYTYKQLYQEVC
                     RTAEMLAAHGVKKGDRVCIYMGMIPELAMAALACARIGAVHSVIFGGFSAQSIVDRVQ
                     DARAEYIITCDGANRGAKEISLKSIVDDALEDLDIIKHVFVHRHTGSETRMIPGRDIW
                     WHEARNESINDYPAEIMDSEDLLFILYTSGSTGKPKGAVHTSGGYMVYTTYTFINVFQ
                     YQPDDIFFCTADIGWVTGHSYIVYGPLCAGATSLMFEGIPTWPDAGRFWDIVDKHKVN
                     ILYTAPTAIRSLMSYGLQPLEGKDLGSLKVLGTVGEPINEEAWFWYHDHVGKNRCPIV
                     DTWWQTETGGILISNLAGVTSARPSYATLPMPGVQPVLVDEKGNEVLGNPAAGNLCIK
                     APWPSIIRTTYRDHERCRLTYFHSYPGLYFTGDGALRDEDGNYRITGRVDDVLNVSGH
                     RIGTAEVENAINEHEGVVESAVVGFPHDIKGQGIYAFVICPEKEGNDQLRKAINATVD
                     LVIGPIARPDRIQFVTGLPKTRSGKIMRRILRKIAEGEMHQLGDTTTLLDPNVVEEIK
                     KGRIE"
     gene            complement(6011..6976)
                     /locus_tag="PKM27_18030"
     CDS             complement(6011..6976)
                     /locus_tag="PKM27_18030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNR09225.1"
                     /translation="MKATSASWGYILTFTGAILFSTKAIMVKLAFLQTTADPVSLLAL
                     RMLFSLPFFLFTGWWAAKKYRAEPLSLKQWIYIAALGLMGYYLSSLLDFVGLQYITAG
                     LERLILFLYPTFTVIINAVFFKESFSRKQVTALVLSYLGIGIAYSHEFALDVNKSGFL
                     LGSGLILLCSVTFAGYIVGSGRLLKKIPAMQYTSLAMLSATLGVMLHFILAGNFSILH
                     LDRQMIRFGLLLAIVATVIPNFMVSAGIQRIGSNNSAIVSAIGPVSTIVLAYFFLGER
                     MSGYQITGTCFVIAGVLLIGWRGGFPRLTWPTKWMAHEDTKYTKN"
     gene            7516..7597
                     /locus_tag="PKM27_18035"
     tRNA            7516..7597
                     /locus_tag="PKM27_18035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7550..7552,aa:Leu,seq:gag)
     gene            complement(8126..8422)
                     /locus_tag="PKM27_18040"
     CDS             complement(8126..8422)
                     /locus_tag="PKM27_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09226.1"
                     /translation="MEHQILTVHRVKFILGIALLLCLLPMPYGYYQLVRFMAMCGFAW
                     LAYQVRDEHEQKMWLYIALAFLFQPFVKIALGRGLWNIVDLVVGIWLIYEVRRK"
BASE COUNT         2115 a         2204 c         2172 g         1990 t
ORIGIN      
        1 acttaattcc ttctatttat ttcttttaca gatatataca acttctataa tactttttta
       61 tatttgtttg cacgaaacct tgaaacacca ttaagcaaac attcagaggc aacggaaaac
      121 tactcatcac aggtgaatac gctgtgctgg acggcgcatt ggcgcttgca gttccaacca
      181 aggcgggtca gacgatgaac gttcgggacg ggcggggcgc ggagatcaac tggaaagcct
      241 acgattccac cggcaaggtt tggtttgaag cccatttttc cctgtacgat ttcagcccgc
      301 tcaaaaccaa cgatccggaa aaagcgaaaa aaataagcaa actgctcaag gaatgcaccc
      361 ggcagaactc ggagtttctg tccgcctgga aaggccagac cgtggaaacc tacctggatt
      421 tcccgctgga ctggggactg ggttcgagca gcaccctgat ctattgcctt tcccaatggg
      481 cagacgccaa tccctattat ctcctgttca atacctgcgg gggctcgggc tacgatatcg
      541 cctgcgccta cgccgaaggc ccggtgtttt accagatggg cgaagaaaat atccggatcg
      601 atgaagtgta cttcaatccg cctttcaagg actcccttta tttcctgcac ctggggaaaa
      661 aacaggacag ctccgcagag gtaaaggaat acaaaatcaa atccaaacca agccaggaga
      721 tcctggataa gatttccgcc atcagtgagg aaatggtcgc ttcgaaagac agcaaacaat
      781 tccaaaaact catggcttcc cacgaggacc tggtgagcgg tttgctggac cgtccgccca
      841 tcaaaaaaca attgttccct gactttaaag gtgccatcaa atcactcggc gcctggggcg
      901 gggactttgc cctggtatgc agcgatgaac cagcttcttc cgtacgctct tattttgaat
      961 ccgcgggata tccgaccctc atccccttca ccgagctggt tttaaacgaa tgaccggatc
     1021 atgcgggcgc actggtacac gggcatagtc accgggatcc acgaacttgc cccccaggta
     1081 agatccttca ccctggaaat tcccgaagtc gatcgcttcg aatttaaagc cggccagttc
     1141 gtcaccttcg acctgccggt cagcgaaaaa aggatcaacc gctggcgcag ttattccatt
     1201 gcctcccctc cggaccaggg aaacaagatc gagctctgca tcgtgcattt tgaaggaggc
     1261 atcggcagtt cctatttttt taatgaaatc aatctgggtt ccgaactaaa atttaaaggg
     1321 cctgaaggcg gttttatact tcctgaagtc ctggaccacg acctcgtcat gatctgtacc
     1381 ggtacgggga tcgctccctt ccgcagcatg cttttagata tcaaacaaag aggggtcggg
     1441 catcaggggc tgcatctgat cttcggcacc cgtaaacagg aagatattct ttatcgggaa
     1501 gaatggtcaa gccttgaaag ggaacttccc ggcttccgtt ttgacatcgc cctgtcccgg
     1561 gagtttccgg gcccggattg ggcatcccat atgcatcagg ggtatgtgca cccggtctat
     1621 caaaaggctt ataccccgta ccggcccgat gtccattttt atctctgcgg ctggaccaaa
     1681 atgatcgatg aggccatcgc caacctgttt gcgggaatgc ggtatccgag ggaacagatc
     1741 cattatgagt tgtatgggtg aaaggggtag atatgccaat ctatgagcaa ttggtctgag
     1801 atttggcata tctgatgcta cccttccaat gtttcaaccg cctccaccgc ctcttcattc
     1861 accatcactt cctccggcct cagttcatcc aggccggtgc gcaggttttc gatgataaat
     1921 tcctggcggt cgggggtgtt cttgcccata tagtattcga ggatgtgttc gatggcggtg
     1981 ccttcctgga gcatgacggg ctccagcctc atgtcgtttc cgataaaccg gccgaattca
     2041 ttcggggaga tttcacccag gcccttaaag cgggtgatct ccggtttacc ccgcaaggcg
     2101 gcaatggcct cttccttttc ccttatggaa taacaataga acgtttgttg tttgtcccgc
     2161 acacggaaca aaggagtttc caggataaag aggtggccgt tgcgcaccag atcgggaaaa
     2221 aactgcagga agaaggtcag taaaagcagg cggatatgca tgccgtccac gtcggcatcg
     2281 gtgctgatca cgacctggtt gaacctcagg ttttcgatcc cgtcttcgat atccagggca
     2341 tgttggagca ggttgagttc ctcgttttca taaacgattt ttttgctcat gccgtaacaa
     2401 ttcaagggct tgccgcggag gctgaacacg gcctgggtct gcacgttgcg ggccttggtc
     2461 agggatccgc tggccgaatc cccctcggta atgaagaggg tggtcgcgag cctcaattcc
     2521 tcatcggcct tatcagaaaa atgcacgcgg caatcatgaa gttttttgtt gaaaatattg
     2581 gcttttttgg ccctttcgtt ggccagtttt cgcaccccgg cgatttcttt tctctccctt
     2641 tccgattgct ggatcctcgc ctggagggcg ctggccgcat ccggatgttt atgcaggtaa
     2701 ttgtccagct ggctggtgat gaattcactc acaaaggatt tgaggctttg cccttcggga
     2761 gcgatggcct gggaacccag cttggttttg gtctgggatt caaacaccgg ctcttcaatg
     2821 cggacgctca ctgcggcgat gatggaggcc cggatgtctt tggggtcgta ttccttttta
     2881 aaatggttgc gcaccgtttc cacaatggct tcccggaagg ccgcgagatg ggtcccgccc
     2941 tgggtggtgt tttgcccgtt gacgaaagaa taatattgct ctccgtattc attcccgtgc
     3001 gtcatcgcga tctcaatatc ctcgcccttg aggtggataa tggggtacct cagttcttcc
     3061 ccttccgttt tgcggccgag caggtccagc aatccgtttt tggataccat caccttgttg
     3121 ttgaacctga ttttcaagcc ggcattgagg taggcatagt tccacaattg gttttcaata
     3181 tgaggcaggt ggtattcgta gtgtttaaat atggaggcgt cgggggtgaa gcgaaccagg
     3241 gtgccgtttt cttcggaggt gccggacagg ggtgggtctt tcacgatcac tcctttgcgg
     3301 aattccgcca gcttggactt gccgtcgcgg aaggactgga taaaaaacag gtccgaaagg
     3361 gcgttgacgg ccttggtgcc gaccccgttg aggcccaccg attttttaaa ggctttggaa
     3421 tcgtatttgg cgccggtatt gatccgggac acgcattcga cgaccttgcc tagcgggacc
     3481 ccgcggccat aatcccggat ttccaccgag ccgccggagc aggtgatgtc gacttgtttg
     3541 ccatagccca tggtaaactc atcgatgcag ttgtcaatca cctccttaat gagcacgtat
     3601 atcccgtcgt ccggggcggc gccgtcaccc agcttgccga tgtacatgcc gggccggagc
     3661 cggatgtgtt ctttccagtc gagggacttg atatcgtctt cggtatattt tacttctgcc
     3721 atgtttgatt ggtttgagcc ggctaaagat aagacatatt ataaaaatca ataatctgag
     3781 aggggccggc ccaaaattct gatttaaaca aataatttat aaacttgtgc aaaattgccg
     3841 gtttttaagt catcaccatc agaaacatta tatctatgaa gattacttca ctggaagagt
     3901 accagaaaac ctataaacgc agcatcgagg acccggaggg gttttgggcg gagatcgcct
     3961 cgcagtttac ctggaagaaa aaatggaata aggtcctgga ctggaatttc acggaacccc
     4021 gggtaaaatg gttcgaagga ggccaactga acattactga aaactgcctg gaccgtcacc
     4081 tggctgacaa accggaccgg acggccctga tctgggagcc caatgatcct gcggaatcca
     4141 cacggcgata tacctacaag caattgtacc aggaagtctg ccgtacagcc gaaatgctgg
     4201 ccgcccatgg agtaaaaaag ggagaccgcg tatgcatcta tatgggcatg atccccgaac
     4261 tcgccatggc cgccctggcc tgcgcccgca ttggagcggt acacagcgtg attttcgggg
     4321 gattcagtgc ccagagcatt gtggaccgcg tacaggatgc gcgcgccgaa tacatcatca
     4381 cctgcgacgg agccaaccgc ggcgccaagg aaatcagcct caaaagcatc gtggacgacg
     4441 ccctggaaga cttggatatc atcaaacacg tatttgtgca ccggcatacc ggttcggaaa
     4501 cccggatgat cccgggcagg gatatctggt ggcacgaagc gcgcaacgaa tccatcaatg
     4561 attatccggc ggagatcatg gacagcgaag atctcctgtt catcctttac accagcggca
     4621 gcacgggcaa acccaaaggg gccgtgcaca cctccggggg ctacatggtg tacaccactt
     4681 atacttttat aaatgttttc cagtaccagc cggacgatat ctttttctgc accgccgata
     4741 tcggatgggt caccggccac agttatattg tctatgggcc gctttgcgcc ggggccacca
     4801 gcctgatgtt tgaaggcatt cccacctggc ccgacgccgg ccgcttttgg gatatcgtgg
     4861 acaaacacaa ggtcaatatc ctgtatacgg cgcccactgc gatccggagc ctgatgagtt
     4921 acgggctcca gcctctcgaa ggcaaagacc tcggcagcct gaaagtattg ggtaccgtcg
     4981 gggaaccgat caacgaagaa gcctggttct ggtaccacga ccatgtggga aaaaaccgat
     5041 gccctatcgt ggacacctgg tggcagacgg aaaccggggg tatcctgatc agcaacctgg
     5101 ccggggtaac ctcagcgcgt ccttcgtatg cgacgctgcc catgcccggc gtccagcctg
     5161 tattggtgga tgaaaaaggc aatgaggttc tgggcaaccc ggcagccggc aatctctgta
     5221 tcaaggcccc atggccatcc atcatccgga ccacttaccg cgatcatgaa cgttgcaggc
     5281 tgacttattt ccattcctat cctggactct attttaccgg ggacggcgcc ctgcgcgatg
     5341 aagacggcaa ttaccggatc accggccggg tggacgatgt cctcaatgtc agcggccacc
     5401 gcatcggtac cgctgaagtg gaaaacgcca tcaatgaaca cgaaggcgtg gtcgaaagcg
     5461 ccgtggtcgg tttcccccac gatatcaaag gtcaggggat atacgctttt gtgatctgcc
     5521 ctgaaaaaga aggcaatgac cagttgcgca aggccataaa cgccaccgtc gacctggtga
     5581 tcggacccat tgcacgtccg gaccggatcc agtttgtgac cggtcttccc aaaacccgca
     5641 gcggaaagat catgcgccgg atcctcagaa aaattgccga aggggaaatg catcagttgg
     5701 gtgataccac taccctgctg gacccgaatg tggtggagga gattaagaag gggaggattg
     5761 agtaaaatgg cgaagggcga acggtgaaag gcgaaaggag caaaaaggtc catacctggt
     5821 ctaatgtcta atcataagta gttacgaggc attcagtttt aaagaaattt acttttgctc
     5881 atggtgccgg tgagggaaag catcaccatt ttttggtgta cttggccttc gtggggcgct
     5941 cccccaggga tccaacttta aaaaattact cccgcacttc gcttcgctgt gccgttatgt
     6001 caaaccctca ctaattcttt gtgtacttag tgtcttcgtg ggccatccac tttgtgggcc
     6061 atgtgagccg agggaaccca ccgcgccaac cgatcaacaa cacccccgcg atcacaaaac
     6121 aagtacctgt gatctgatac ccagacatcc tctcccccag gaaaaaataa gccagcacga
     6181 tggtgctcac cggaccgatg gccgatacga tggccgagtt gttggaacca atacgctgga
     6241 tacctgccga gaccataaaa ttgggaatca ccgtggctac tatggcgagt aataaaccaa
     6301 accggatcat ctggcggtcc aggtgcagga tggaaaaatt accggccagg ataaaatgga
     6361 gcatcactcc aagggtggcg ctcaacatgg cgagagaggt atattgcatg gcgggaatct
     6421 ttttgagcag acgacctgaa ccaacaatat atccggcaaa agtgacggaa cacaaaagga
     6481 tgagcccact gcccagtaaa aacccggatt tattcacatc gagggcaaac tcatgcgaat
     6541 aggctatccc tatccccagg taggagagga ccagggcggt cacctgtttc cggctaaagg
     6601 attctttgaa aaacaccgcg ttgatgatca cggtgaaggt gggataaaga aagaggatca
     6661 gccgctccag gcccgcggtg atgtattgga ggcccacaaa atccagcagg ctgctcaggt
     6721 aatagcccat gaggcccaag gcggcaatat aaatccattg tttcaggctc aggggttccg
     6781 cccggtattt tttagctgcc caccagcccg taaacaggaa aaaaggcagg gaaaaaagca
     6841 tccgcagggc caggagggat accgggtcgg ccgtggtctg caggaatgcc agtttgacca
     6901 tgatggcctt ggttgaaaat aaaattgccc ccgtaaaagt gaggatatag ccccaggaag
     6961 cggacgtggc tttcattgcc ttaaaggtca gaatttaagt gcctggaatt caatgaattt
     7021 taaggcaaac tccggtggca aacagcaatt gtaaagattt tcataggcca ggctgcgtat
     7081 gacaattcta caattccacg attgagcaat tcgacaattc cactatcttt ccccccccta
     7141 aacgcccggg tggtgaaact ggtatacacg tcctgacgac gccggtggcg tgtcaggatg
     7201 ctgaccgaag cgtcagcata cgtttgaggt cctccccaat cacccactct tctaacattt
     7261 taacccattt tgcttctttg ttcaggaaaa tataaattct gcagaaagta tatctttgca
     7321 ttcctaaacg cccgggtggt gaaactggta tacacgtcct gacgacgccg gtggcgtgtc
     7381 aggatgctga ccgaagcgtc agcatacgtt tgaggtcctc cccaatcacc cactcttcta
     7441 acattttaac ccattttgct tctttgttca ggaaaatata aattctgcag aaagtatatc
     7501 tttgcattcc taaacgcccg ggtggtgaaa ctggtataca cgtacgtttg aggtgcgtat
     7561 gccgaaaggt gtgcaggttc gagtcctgtt ccgggcactt tgttctttgg caggtcctga
     7621 cgccccgtag ggggtcagga tgctgaccgg ctttggccgc gtcagcatcc gagtcctgtt
     7681 ccgggcactg gaagccatcg agagatggct tttgtatttt gatgacacac tatacctaca
     7741 tactttattc ttccaagttc gaccgttatt acattggtca aactaatgat atccacaagc
     7801 ggcttcaacg acataacaat gggactgaac cggcgacaaa accctatata ccctgggaat
     7861 tattatggtt tacctcaaaa agtagcagac aagagtcaat ggctttggag aaaaaattaa
     7921 aaaacctatc aaaagcacga atcagaacat tcatagctaa gtaccctagt gcagttcctg
     7981 acgccctaaa ggggtcagga tgctgaccgg ctttggccgc gtaagcattc aagtcctgtt
     8041 ccgggcactg gaagccatcg aaagatggct tttgcatttg tatgagaccc ttttcctaaa
     8101 ctaacggctc actgcccctc cccactcact tcctcctcac ctcataaatc aaccatatcc
     8161 ccaccaccag gtcaacgatg ttccaaagtc ccctgcccaa ggcaatttta acaaatggct
     8221 ggaaaagaaa ggccagcgcg atatataacc acatcttctg ttcatgttca tccctgacct
     8281 ggtaggccag ccaggcaaag ccgcacatag ccataaaacg taccagttgg taataaccat
     8341 agggcatggg caaaaggcag agcaggaggg caatgcccag gataaattta acccggtgta
     8401 cagtaaggat ttgatgttcc ataagttaga attcaaatat accaaatccc agggcatgtt
     8461 taaaatcagc ttccccaaag g
//
LOCUS       DAOQXT010000155         8403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_8940_length_8403_cov_6.556181, whole genome shotgun sequence.
ACCESSION   DAOQXT010000155 DAOQXT010000000
VERSION     DAOQXT010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8940_length_8403_cov_6.556181"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKM27_18045"
     CDS             <1..363
                     /locus_tag="PKM27_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09227.1"
                     /translation="LGSQSLVLSAKGGWLAHWIRFQNPGNTFIPIQGEYRAGYDRFSS
                     GFALGEQLAYRHLAKNRLINFEIALQFSQAFTRLKRNIQLDNPEVKPINRFDGLAGLQ
                     ARWIIPFYKKKDPGTLYY"
     gene            375..1586
                     /locus_tag="PKM27_18050"
     CDS             375..1586
                     /locus_tag="PKM27_18050"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNR09228.1"
                     /translation="MHFLYNTAIRIFALALPFLSLLNKKWNKFYRGRKNQRAPRFKTS
                     PVWVHCASLGEFEQGRPVIEAIKKEFPGIPLVLTFFSPSGYEIRKDYPFADWVAYLPL
                     DTPSKAAGFVDHIRPRLAIFVKYEYWFNHLLALEKADVPFVFIANVWRRHYFPLKPWA
                     AWFLQVIRKGSCFFAQDQLTMDLLSEKGFERVVQAGDTRIDRVLQLVENKKILPEVEK
                     WKGSKTTLIAGSTWPSDERLIAGFLERHPEITLILAPHEPSPSRIEDIRKGFAEFLPI
                     RYSEWKGEDARVIVVDSMGLLNALYAYGDAAYIGGGFGRGIHNTLEAAVYGIPVFFGP
                     RHQNFKEAIDLIGLGLAFEVNTVNELVQQWEQIVPSELPRIKGQVRDYFDQNRGATAK
                     IIDYLRPLIQH"
     gene            1588..2556
                     /locus_tag="PKM27_18055"
     CDS             1588..2556
                     /locus_tag="PKM27_18055"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HNR09229.1"
                     /translation="MSVTEALDDFESKKILVIGDIILDIYIHGTVERISPEAPIPIVN
                     HQHTRHALGGAANVAANLKGLGADVTLVSATGEDREAEYLGVMLDLEEIAHDLIAVKD
                     RPTTSKSRIIAAGHQMLRIDHEMTEELSPEQSKLLLEKILDRLRQEPPAALVLEDYNK
                     GLLTEEVIHTVLDKARSLAIPVIVDPKQSHFFAYSGCRVFKPNFKECARQLPFPLKPE
                     MESLQQADAYLRGRLHHGISMITLAEHGIFISDGHRSHLLPTEIRQVSDVSGAGDTVT
                     AIATFGIVQSLSLELIASLANKAAGLVCARPGVYAIRKEELIQNHN"
     gene            2590..4860
                     /locus_tag="PKM27_18060"
     CDS             2590..4860
                     /locus_tag="PKM27_18060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR09230.1"
                     /translation="MLRNLILLTFLGFAVGLNAQTVFRVTANGITTEYGFEVAEFGGQ
                     LPDSVNSYDLILANNGVNNSQGCDTILPVTGKIVLVDRGTCSFIQKALKAQRAGAVMA
                     IICNNQGGDDIFPPGGFDPAVTIPTIMVGQNDCIKIKMNLSGSKAKFIFEDPTNDTQI
                     HYYEDFDSGPDGWTTKGLSNASDTFVWTKNGVSNGVISYVGGLGSLIIDAPSKNNGSM
                     LFDAAFLTTQGDTNNIPGGPAPYARRYGELISPVINCSTFNSVTLKFFEAIATLNSFD
                     NGTSSYATSIDGGATWSNPVEINTDLQPNETTPRGKFLKIELPQLANQAQARIKFIFD
                     SDFYAWIIDDVYLLGKSPVDIEVQDKQFFFPFNYATPASQITTDTSTFSANLANLGSL
                     DISNLTLRAQLFSSTGALLHNDSLVIANLPSGARDTTFSLPKSFVPGKLDPGVYFIVY
                     SVEPPPGSPDDADPTNNVRIQEFIVTTNLYSKDDGLNTSQGIRAANGGDYFFGNLYYT
                     STDWNANDKFSASNVTFGAFMVQADGMLKGKSVTIYLAELLPSVDRDFNNFDVNKAIT
                     DNPDQIKIVGFATHEFTEDQSEIAEETLLDFVNFTDNVPLKKGTRYLLGVSYKDAASK
                     AYHFIESDIQYYYIGTLLYAGGQWGGSSDAPVMRMELEISTPVDEVALPEQTLQLMPN
                     PASDYIRAQVNFDKLTNANFVIADINGRVLQLHTKKGVLKETFEFDTHNLAPGTYLMR
                     ISTDDGTKTKKFIIQR"
     gene            complement(5047..7713)
                     /locus_tag="PKM27_18065"
     CDS             complement(5047..7713)
                     /locus_tag="PKM27_18065"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNR09231.1"
                     /translation="MELASRYQPAEIEDKWYAHWMERGYFNSVPDERESFSIVIPPPN
                     VTGVLHMGHILNNTIQDVLIRKARMEGKNACWVPGTDHASIATEAKVVRWLREEKGLK
                     KGDIGREEFMQYAWQWTEKYGGIILTQLKKLGASCDWNRTAFTMDESRSEAVYKVFVD
                     LYRKGKLYRDYRMVNWDPEAKTVLSNEEVLYQEENAKLYYVSYPIEGRPGEFITIATQ
                     RPETIMGDTAVAVHPEDDRYLHLEGFKAIVPLVDRPVPIIFDEYVDPAFGTGALKVTP
                     AHDTNDYELGKKHKLETIDTLNPDGSLNEAARFFVGMDRFEARKKVVEKLDELGLLQK
                     AEDYQTNIGRSERTNAVVEPRLSLQWYVDMKKLAAPALSAVMTDEILFIPEKYKNVYR
                     HWMENIRDWCISRQLWWGHQIPAWYLSDSSHEQPALSQEPFVAESIEEALDMAKQRTG
                     NHSLTIQDLQRDEDVLDTWFSSWLWPITVFDGFRSRKDLEYYYPTSVLVTGWDIIFLW
                     VARMIMSGYEWEGKMPFKKVYFTGMVRDKLRRKMSKSLGNSPDCLQLIADYGADAVRY
                     GILASSPAGGDLLFDEKLLENGRNFCNKIWNATRLVKGWQVDPEAEIQPVQQLALRTF
                     AHKWNKCKSEINEDIRQYRLSEAVTALYSFIWDDFCSWYLEMVKPEAGKGMAAIVREE
                     SIGFFEELLSALHPFLPFVTEELWHQLRDRKPGDDCILSPYPGPETYDESLISTTGSL
                     QEIIRSIREIRNKNGVSMKDSLVLKIFHSEEGKSLFDEEPQVRFVSKFGNLSAVEWVS
                     EEVPGAASFINGTTKYFLLLNKQVDPVAEKTRLEKELEHARGFVASIRKKLENERFVQ
                     NARPEVIENERKKLDDGLERLKGIEQSLHSFQ"
BASE COUNT         2055 a         2218 c         2005 g         2125 t
ORIGIN      
        1 ctgggcagcc agtccctggt cctgagcgct aaaggcggct ggttggccca ctggatccgc
       61 tttcaaaatc ctggaaatac cttcatcccc atacagggag aataccgggc gggatacgac
      121 cgtttttcct cgggctttgc ccttggggaa cagctggctt accggcatct ggccaaaaac
      181 cggcttatca attttgaaat tgcccttcaa ttcagccagg cattcacccg gctgaaaaga
      241 aatattcaat tggacaatcc cgaggtcaag cctataaacc gttttgatgg cttggccggc
      301 ttgcaggccc gatggatcat tcctttttac aagaaaaaag acccgggcac cctttattat
      361 tgacttttcg attcatgcat ttcctctaca atacggccat tcggattttt gcccttgcct
      421 tgcccttcct ctccctcctg aataaaaaat ggaataaatt ttacaggggc agaaaaaatc
      481 aacgcgctcc ccggttcaaa acatccccgg tatgggtgca ttgcgcttcc ctgggagaat
      541 ttgaacaggg ccggccggtc atcgaagcca ttaaaaagga gtttcccggc attcctttag
      601 tcttgacttt tttctcccct tccggttatg aaataaggaa agattaccct tttgcggact
      661 gggtggctta cctgcccctg gatacccctt cgaaggcggc cggtttcgtc gatcatatcc
      721 gtccgcgact ggccatcttt gtcaaatatg aatactggtt taatcatttg ctggccctgg
      781 agaaggcgga cgttcccttt gtattcatag ccaatgtctg gcgccggcat tatttcccgc
      841 tcaaaccctg ggccgcctgg tttttacagg tgattcgaaa gggaagctgt ttttttgccc
      901 aggatcagct gaccatggat ctcttaagcg aaaaaggttt tgaacgggtg gtccaggccg
      961 gggatacccg aattgaccgg gtccttcagc tcgttgaaaa caaaaaaatc ctgcctgaag
     1021 tggagaaatg gaagggttct aaaaccaccc tgatcgccgg gagcacctgg ccctccgatg
     1081 aacggcttat agcgggcttc ctggaaaggc atccggagat caccttgatc ctggctcccc
     1141 atgaaccctc cccttccagg atcgaagaca tccggaaggg tttcgctgaa tttttgccga
     1201 tccgctattc cgaatggaag ggggaagatg cccgggtgat cgtggtggac agcatgggtt
     1261 tgctcaacgc gctttatgct tatggcgatg cggcatatat cggcggagga tttggccggg
     1321 gcatccacaa tacgcttgaa gccgccgtgt atggaattcc tgtttttttt ggtccaagac
     1381 atcagaattt taaggaagcc atcgacctga tcggcctcgg tctggccttc gaagtcaata
     1441 cggtgaatga actggttcaa caatgggagc aaatcgtacc ctcagagctg ccccggataa
     1501 aaggacaagt ccgcgattat tttgatcaaa accggggagc tactgcaaaa atcattgact
     1561 accttcgccc cctcatccag cattaaaatg tcggtaacgg aggctttaga cgatttcgag
     1621 agtaaaaaaa tactggtgat cggagatatc atcctggata tctacattca cggtaccgtc
     1681 gaaagaattt ccccggaagc gcccatcccg atcgtcaacc accaacatac ccgccatgcc
     1741 ctgggaggcg ccgccaatgt ggctgccaac ctcaaaggtc tcggcgcgga tgtgaccctg
     1801 gtatccgcca ccggggaaga ccgggaagcg gaatacctgg gcgtcatgct ggacctggag
     1861 gaaattgctc atgacctcat cgcggtaaag gaccgcccca ccacctcaaa aagcaggatc
     1921 atcgccgcgg gacaccagat gctgcgaata gaccatgaaa tgaccgaaga actttctccg
     1981 gagcagagta agctattgct tgaaaaaatc ctggaccgcc tgcggcagga gcctccggca
     2041 gccctggtcc tcgaagacta caataaagga ctgctcaccg aagaagtgat tcacaccgtc
     2101 ctggacaaag caagatccct tgctatcccg gtgatcgtcg atcccaagca gagccacttt
     2161 ttcgcctatt ccggttgccg ggtattcaag ccgaatttta aggaatgcgc ccgccaattg
     2221 ccctttccct taaagcccga aatggagagc ctgcagcagg ctgatgccta tctcaggggc
     2281 cggcttcatc acgggatcag catgatcacc ctggccgaac atggaatctt tatttcagac
     2341 ggccacagga gtcacctgct gcctaccgaa atccggcagg tctccgacgt cagcggagcc
     2401 ggtgataccg tcaccgccat tgcaacattt ggcatcgtcc agtcgttatc tcttgagctt
     2461 atcgccagcc tggccaacaa ggctgccgga ctggtttgcg cccggccggg tgtgtatgcc
     2521 atccgcaaag aagaacttat tcaaaaccac aattaatact atttttgaat cctaaaacct
     2581 aattttctga tgctccggaa tttaatctta ttgacatttc ttggctttgc agtgggcctc
     2641 aatgcccaga ctgtattcag ggtgactgct aacgggatca ctaccgaata tggatttgaa
     2701 gttgcagaat ttggtggtca acttcctgat tccgtgaatt cctatgatct tattcttgca
     2761 aataatggcg tcaacaactc ccaaggttgc gataccattt taccagtcac cggcaaaatt
     2821 gtcctggtgg atcggggcac ctgttcattc atacaaaaag ccctaaaagc ccaaagggca
     2881 ggggcagtga tggcaatcat ttgtaacaac cagggcgggg atgacatttt tcctccaggt
     2941 gggtttgatc ccgcggtcac gatccctaca attatggtgg gtcaaaatga ttgtatcaag
     3001 atcaaaatga acctttcagg aagcaaggca aaattcatct ttgaagatcc caccaatgac
     3061 actcaaatcc attattatga agattttgac agcggtccgg acggctggac cacgaagggg
     3121 ctatccaatg catcggatac ttttgtgtgg acaaaaaatg gggtgtccaa tggagtaatt
     3181 tcttatgtcg gaggcttagg ttcacttatc attgatgccc ccagtaaaaa caatgggtct
     3241 atgctgtttg atgcagcatt cctgactacc cagggcgata ctaataatat cccaggcggt
     3301 ccagccccat atgcaagacg ttacggagaa ttgatctcac cggtcatcaa ctgcagcacc
     3361 ttcaattcgg tcactttgaa attttttgag gccattgcta ctttaaattc atttgataat
     3421 ggaaccagtt catatgctac ttccatcgat ggaggggcta cctggtcgaa ccccgtggag
     3481 ataaacacgg atcttcaacc caatgaaacg acgcccaggg gaaaatttct taagatcgag
     3541 ttacctcaac tggccaacca ggcccaggcc aggatcaagt tcatttttga cagcgacttt
     3601 tatgcctgga tcatcgatga tgtttacctg cttggtaaat ccccggtgga tattgaggtc
     3661 caggacaaac aatttttctt ccctttcaac tatgctactc cggccagcca gatcacgacc
     3721 gatacctcca ccttcagcgc caacctggcc aacctgggat cgttggatat cagtaatttg
     3781 accctgcgtg cccaactgtt cagcagtacc ggcgccttgt tgcacaacga ttccctggtc
     3841 attgccaacc tgccttccgg cgcccgggac acgacgttct cacttcccaa gtcctttgta
     3901 cctggcaaac ttgaccctgg cgtttatttt atcgtttatt ccgttgaacc tccgcccgga
     3961 tcgccggacg atgcggaccc gaccaataat gtgcggatcc aggaatttat tgtgaccacc
     4021 aatctctatt ccaaggatga cggtttgaat acttcccagg gaatcagggc cgccaatgga
     4081 ggcgattatt tttttggtaa tctctattac acttccacgg actggaatgc caatgacaag
     4141 ttcagcgcca gcaacgtcac tttcggagct tttatggtac aggccgacgg catgctcaaa
     4201 ggcaaatcgg tcaccatcta cctggctgag ttgctgccga gcgtggaccg cgatttcaac
     4261 aattttgatg ttaataaggc tatcacggac aatccggacc agatcaagat cgtgggtttc
     4321 gccacccatg aatttaccga agaccaaagc gaaatcgctg aagagaccct gcttgatttc
     4381 gtaaatttca cggacaacgt tcctcttaaa aaaggcaccc gatacctatt gggcgtctcc
     4441 tacaaagatg ccgccagcaa ggcttatcat tttatagagt ccgacatcca gtattattat
     4501 atcggtacct tgctgtacgc aggcggccaa tggggaggat cttcggatgc gcccgtcatg
     4561 cggatggaac ttgaaatttc caccccggtg gacgaggtgg ccctgccgga gcagaccctg
     4621 caactcatgc ccaatcctgc aagcgactat atccgcgccc aggtcaattt cgataaatta
     4681 accaatgcca attttgtgat cgccgacatc aacggaaggg tcctgcagtt gcatactaaa
     4741 aagggtgtcc tgaaagagac ctttgaattt gatacccaca atctggctcc gggcacctac
     4801 ctgatgcgga tctctacgga tgacggaacg aagacgaaga agtttattat acagaggtaa
     4861 ttcgtagata tcagctgtca gcaatcagct gtcggctatc agctgtcagc tatcagctat
     4921 cagctatcag cgatcagcca tcagctatca gccatcagct atcagccatc agctatcagc
     4981 tagcagctat cagctgaaag ctgacaacta aatgctgact actgaaagct gaatgctgaa
     5041 agctgactac tgaaagctgt gcaggctttg ttcgatgcct tttagcctct ccagcccatc
     5101 gtccagtttc ttcctctcat tctcgattac ctcgggcctg gcattctgga caaaacgttc
     5161 gttttcgagt tttttacgga tggaagccac aaaaccgcgg gcatgttcca gttctttttc
     5221 caggcgggtt ttttccgcaa cgggatctac ttgtttattg agtaataaga aatatttggt
     5281 cgttccgttg atgaaggagg ctgcaccggg cacctcttcg gatacccatt ccacggcgct
     5341 caggttaccg aatttgctta cgaagcggac ctgaggttcc tcatcgaaca aggacttgcc
     5401 ctcctccgaa tggaagattt tgaggaccag ggagtctttc atgctgactc cgtttttatt
     5461 ccggatctcc cggatcgacc tgatgatctc ctgtaaagaa cctgtggttg aaatcaggga
     5521 ctcgtcatag gtctccggtc ccgggtaagg gctcaggata caatcgtcac cgggtttccg
     5581 gtcccggagc tggtgccaga gttcttccgt gacaaaagga aggaagggat gcagggcgga
     5641 caacaattct tcaaaaaatc cgatgctttc ttccctgacg atggctgcca tgccttttcc
     5701 ggcctcgggt tttaccatct cgagatacca gctgcagaaa tcgtcccaga tgaaggaata
     5761 cagggccgta acggcttcag acaatcggta ttgacggata tcctcattga tttccgattt
     5821 gcatttgttc catttatggg caaaggtccg gagggcgagt tgttgaaccg gttggatttc
     5881 cgcctccgga tccacctgcc atcctttgac caggcgggtc gcattccaga ttttgttgca
     5941 gaaattgcgg ccgttttcaa ggagtttttc atcgaataaa agatcgccgc ccgcagggct
     6001 ggaggccagg atgccgtatc gcaccgcatc tgccccgtag tccgcgatca gttgcaggca
     6061 atccggacta ttgcccaggg acttgctcat cttccgcctc agtttatccc tgaccatgcc
     6121 cgtgaaatac acttttttaa agggcatctt tccctcccat tcataaccgc tcatgatcat
     6181 ccgcgccacc cataaaaaga tgatatccca gccggtgacc aatacggaag tcggataata
     6241 atattccagg tccttgcggc tcctgaatcc gtcaaacacc gtgatcggcc acaaccagct
     6301 cgaaaaccag gtatccagca catcttcatc ccgttggaga tcctggatag tcagggaatg
     6361 atttccggtc ctttgttttg ccatgtccag cgcttcctca atcgattccg ctacaaaggg
     6421 ctcctggctc aaggctggct gctcatggct cgaatctgaa agataccagg ccggaatctg
     6481 atgcccccac cacaattgcc tggagatgca ccagtcccgt atgttttcca tccagtggcg
     6541 gtagacgttc ttgtattttt cggggataaa cagtatttca tccgtcatca ccgctgaaag
     6601 ggccggggcg gccagttttt tcatatccac ataccactgc agggaaagcc ggggctccac
     6661 tacggcattg gttctttcgg aacggccgat attggtctgg taatcttcgg ctttttgtaa
     6721 caagccgagc tcatcgagtt tttccaccac ctttttacgg gcttcgaacc ggtccatacc
     6781 gacaaaaaag cgggcggctt cattcaggct gccatcggga ttgagggtat cgatggtttc
     6841 cagtttgtgt tttttgccca attcataatc gttcgtatcg tgggccgggg tcaccttgag
     6901 cgcccccgta ccgaaagccg gatcgacgta ttcatcaaaa atgatcggga caggacggtc
     6961 aaccagcggg acaatggcct taaagccttc cagatggagg tacctatcgt cctcaggatg
     7021 cacggccacc gcggtatccc ccatgatggt ttccggccgc tgggtcgcta tggtgatgaa
     7081 ttcacccggt cttccttcga tcggataaga aacgtaatac agtttggcat tttcctcctg
     7141 gtagaggact tcctcattgc ttaaaacggt tttggcctca gggtcccagt tgaccattcg
     7201 gtaatcccgg taaagtttcc ctttccggta cagatcgaca aaaaccttgt aaaccgcttc
     7261 acttcgcgat tcatccatgg taaaggccgt ccggttccag tcacaacttg ctcccagttt
     7321 tttcaattgg gtcaggatga tgcccccgta tttttcggtc cactgccagg catattgcat
     7381 aaattcttcc cggccgatat cgcctttttt aagccctttt tcttccctga gccagcgtac
     7441 caccttagcc tcagtcgcta tagaggcatg gtccgtaccc ggcacccagc atgcgttctt
     7501 cccttccatc ctagccttcc ggatcagcac atcctggatg gtattgttca gaatatggcc
     7561 catgtgcaaa accccggtga cattgggcgg ggggatcaca atgctgaaag actcccgttc
     7621 atccggtacg gaattaaaat aaccccgctc catccaatgc gcgtaccact tgtcttcaat
     7681 ctcggccggc tgatatcttg acgctagttc catggggggc aaagataagg aacggctgac
     7741 agcgacgaga tgcgagatgc gagatgcgag atgcgagatg cgagatgcga gaacggtcaa
     7801 cggtgaaagg cgaaaggcga agggcgaagg gagcaaaatt acatttgaaa aggttagggg
     7861 ttcggaagct ccaaaatata acttactaat aatcaaccga cttcgtgttc ttcgcttcgc
     7921 tgcgtctttg tgggaaacat ctttgcggga cacctttttt gattaatttt taactgcgaa
     7981 gggtgcggag tacgcagagg tcccacagaa aaatggtgtc ttagtggaaa accaccaccg
     8041 tggctttgtg gaaaaccttt taaagttaag atctcaagca tcgaagatgc tccacgtttt
     8101 taatactcat tcaaagcaaa accccggctt cggagaagcc cgacatcttc gaacataaat
     8161 atttaaaggt cttatcggaa accccaccca aacgatggcc accgccctca tctttatcga
     8221 aaaaaagaat gaaaattttt ttattgggac tgcaatagtt gggcttctcc gaagccattg
     8281 ttttgttctt attttttacc aataaggtca tgcatctccg atgccggaag aattaattcc
     8341 ttgctccgcg aaccctgagt ccctctgcgg ttcagaaaat ggaattaaat cgccgggcag
     8401 cga
//
LOCUS       DAOQXT010000156         8287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_9103_length_8287_cov_5.829932, whole genome shotgun sequence.
ACCESSION   DAOQXT010000156 DAOQXT010000000
VERSION     DAOQXT010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9103_length_8287_cov_5.829932"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="PKM27_18070"
     CDS             <1..1324
                     /locus_tag="PKM27_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09232.1"
                     /translation="TIASMKAGEEIVINFIQEQAFLTDSKALNLDRLNLPGFVKRFER
                     GAHLVCRILSYNAGEKKLFVEVLRYAPDYSAFTEYQDMVADALDGVESVVFRSIDTQK
                     LLSCMKGPEPVLRDGEGRGEHRKPNRAIPEESEWSYLMPGFPDEPGTPDGQEGPAGFP
                     GPAEKQTLQEEYGFFWKDLRFAFGAVQVLCKSAKLGRRVEVKIYNDHLRPEFDAIKNY
                     FGKILKAKKIKVRVRYELEDHRIVHLQAYSPEIARIDRDLIDSVKLEWVKALPKKKIA
                     AEVDKSLFTMDEYFEAATDQKIKPGLFYQDEKELLQDLLDISSSKHYKQLRFLSSNHA
                     HRIMKLRFILHPFSFLFLIEGEKKYHVVWETLDTAEATYVWHIEKDLSELKRTMQKVE
                     KIIETIKVQGKTAYIQNADEHYHRIYHDYSALVDGFVRWKGELEAVLS"
     gene            complement(1463..2701)
                     /locus_tag="PKM27_18075"
     CDS             complement(1463..2701)
                     /locus_tag="PKM27_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNR09233.1"
                     /translation="MNFNALSRQREIYVHGVAGIKPLIPIDFPSLELAAKQKLSSEAF
                     AYIAGGAGLEFTMQANRSAWDKYAIVPRMLKSVGEATLSRTLLGHSYPLPFFLSPIGV
                     LELAHPEGDLAVARACKETGVPMMVSNQASHSMEEISRVLAGSPWFFQLYVSRSDELV
                     SSFVKRAEQAGANGLVITLDTTMLGWRPRDLNLAYLPFLQGKGIAQYTSDPVFQKLCR
                     ELPPDQNKKKINLHSIAGLLRLAAKVPGPYFENLRGKALDQVRTFTSIYSRPDLDWEA
                     IEKIKSSTRLPVILKGIQHPDDAVRAMNAGIDAIYVSNHGGRQVDGAIGSIDALAAIS
                     PVVKNKIPVLFDSGIRCGADILKALALGASMVGIGRPYAYALALAGARGVEELILNLA
                     ADFELTMALAGGQEPMVNGQ"
     gene            complement(2705..3562)
                     /locus_tag="PKM27_18080"
     CDS             complement(2705..3562)
                     /locus_tag="PKM27_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09234.1"
                     /translation="MGILDPLKKLLFGVKGVTKHQAEKAVDFTKEKGEELFDKTSEFV
                     KEAGEKIGKTGAKILEKVDDLWDTTPKPETPFENKPEEPAKQPASEFVEPIRKTMGQA
                     GEKIAEGASNLKEEAKELGGKVVEATDDFWKKAEQFSENITDKAKTKGSELYEKAKTV
                     VEEQARSINQKIDEFIEKEKALEAKEPKGEFGEKPISENKNLTDPLMGKHEDFFDKAK
                     KFLDDQDKKGQKPINVEVKKVEPAPAEEKPLDPKDQKQLNDLLSNSTTPKPDDHIEDA
                     DIVDEGPKT"
     gene            complement(4200..5594)
                     /locus_tag="PKM27_18085"
     CDS             complement(4200..5594)
                     /locus_tag="PKM27_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09235.1"
                     /translation="MDSIAEEIIHTDEFERQPVPPSRWKDWKSFLGMYAGEHVAGTEF
                     MIGPLFLTSGVSAFDLIVGLLLGNFMAVICWRFLTAEIAVKNRLTLYYQLEKICGKNL
                     VTFYNIANGVLFCFLAGAMITVSATAVGIPFNMEMPKLEDTLPNSLTWVIICLVLGAL
                     VSIIAAKGYNTVAKAANWMAPFIVIAFMACGIVALGQLGVKNFSDFWAIWGTGSEPFP
                     GQIKYTFWHVTLWSWFCNAAMHIGMSDLSVLRFAKKASVGWTTAAGMYVGHYLAWIAA
                     ALLYAVYLKSPEAQGMLSAGVAPPVAPGPLAYNALGVIGSLTVILAGWTTANPTIYRA
                     GLAFQAIIPRASTFWVTILAGAIATIAGIFPAFAMKLLGFVALYGFILAPVGAVIVFE
                     HFFAGKADIETNYAEKKGIHFNLAVLLAWGLSFGIFYSISVSQGVFLSFLTLPAWLLC
                     GLLHLGISRYMQKR"
     gene            complement(5607..6758)
                     /locus_tag="PKM27_18090"
     CDS             complement(5607..6758)
                     /locus_tag="PKM27_18090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNR09236.1"
                     /translation="MTWSYNTNYPGIDDLRNAARRRIPRFAFEYLDGGCNEDVNLGKN
                     TSDIREVELKPYYLGAYTPSDLKTELFGQVYDAPFGISPIGLQGLVWPNSPEILARAA
                     TEHNIPFTLSTVSTSSIERIAEITGGKAWFQLYYPSAEEVRTDILRRVEAAQLPVLVL
                     LSDVPTFAFRPRDIKNGLALPPRMTLRNIFQILGKPNWALRTLYHGAPNFATMKQYMP
                     GGLNMKQLGQFMNMTFSGRLSEERIGPIRDRWKGKLVIKGVASEEDTETAIRLGVDGI
                     IISNHGGRQLDAGESTLHSLVALQKKYKGKIKIMLDSGVRTGPDIARVLASGADFVFL
                     GRTFMYGVAALGNEGGNHTISILKIQLKQILEQVCCTHPSELYRHLIPK"
BASE COUNT         2019 a         2035 c         2175 g         2058 t
ORIGIN      
        1 gactattgct tccatgaaag cgggagagga gatcgtgatt aattttatcc aggagcaggc
       61 ttttctcacc gactcaaaag cgctgaacct ggaccggttg aatctgcccg gttttgtcaa
      121 acgatttgag cggggcgccc acctggtttg ccgcatcctt tcttataatg cgggagagaa
      181 aaaactgttt gtggaagtcc tccggtacgc gcccgattat tcggcgttca cggaatatca
      241 ggacatggtc gcggatgccc tggacggggt ggaaagtgtc gttttccggt ccatcgatac
      301 gcaaaaatta ttgtcctgca tgaagggacc tgaaccggta ttgcgcgatg gggaaggaag
      361 aggtgaacat cgcaaaccaa accgtgcgat ccctgaagaa tcggaatggt cgtatttgat
      421 gccgggtttt ccggacgagc ccggaactcc ggatgggcag gaaggtcccg caggattccc
      481 gggacccgct gagaagcaaa cgctgcagga ggaatatggc tttttctgga aggacctgcg
      541 cttcgccttc ggggccgtcc aggtgttgtg caaatccgcc aaacttgggc gccgtgtgga
      601 agtaaagatc tacaacgatc atctcaggcc cgaatttgac gccatcaaaa attatttcgg
      661 aaagatcctc aaggccaaaa aaataaaagt ccgggtccgg tacgaactgg aagatcaccg
      721 gatcgtccac ctgcaggcct attcccctga aatcgctcgc atcgaccggg acctgatcga
      781 ttcggtaaaa ctggaatggg tgaaggccct cccgaaaaaa aagatagccg ccgaggtgga
      841 caaatcgctt ttcaccatgg atgaatattt cgaagcggcc accgatcaga aaatcaaacc
      901 cggccttttt taccaggacg aaaaagagtt gcttcaggac ctgttggaca tttcctcttc
      961 aaaacattat aaacaactcc gtttcctgtc ctccaaccat gcccaccgga tcatgaaatt
     1021 gcgttttatc ctgcatccct tttctttcct ttttctcatc gaaggcgaaa aaaaatacca
     1081 tgtggtctgg gagaccctgg acaccgccga agccacctat gtctggcata ttgaaaagga
     1141 cctgtcggaa ttaaagcgga ccatgcagaa ggtcgaaaag atcatcgaaa ccattaaagt
     1201 gcagggtaaa acggcctata tccagaacgc cgatgagcat taccaccgca tttaccatga
     1261 ttattcggca ctggtggatg gatttgtgag atggaagggg gaattagaag ctgtactgag
     1321 ttaggcctga tcttctaatt actgaccttt aggtatatgg gtgaacggtt aagggtcaat
     1381 ggttaaggga gcaaaaatac ttgatgagat acgagatgcc agtagcgaga tgccagcagc
     1441 gatatgcgag attgactatt gactattgac cattgaccat tggctcctgc ccccccgcca
     1501 gggccatggt caattcaaaa tccgcggcca ggttcaggat caattcttca acgccccttg
     1561 ctccggcgag cgccagtgca taagcataag gcctgccaat gcccaccatc gaggcgccca
     1621 atgcgagtgc cttgaggata tctgctccgc agcgaatacc gctgtcaaat aaaacaggga
     1681 ttttattttt caccaccggg ctgattgctg ccagggcatc tattgatcca atggcgccat
     1741 ccacctgcct tcccccgtga ttggacacgt agatggcgtc gatcccggca ttcatggccc
     1801 ggactgcatc atccggatgt tggatgcctt tgaggatgac gggaagacgg gtgcttgatt
     1861 taattttttc gatcgcttcc caatcaaggt ccggccggga gtaaatggaa gtaaaggtcc
     1921 gcacctgatc cagcgctttc cctctgagat tttcaaaata agggccggga actttagcgg
     1981 ccagtcgcag caggccggcg atgctgtgca ggttgatctt ctttttgttt tgatccggtg
     2041 gcagttccct gcataatttt tgaaaaacag gatcactggt atattgcgca atgcctttgc
     2101 cctgaaggaa gggcaggtaa gccaggttga ggtcacgggg ccgccagccc agcatggtgg
     2161 tatcgagggt gatcaccaat ccatttgccc cggcctgttc ggcccttttt acaaaactgc
     2221 tcaccaattc atcggaacgg ctgacataca actgaaaaaa ccagggactg ccggccaata
     2281 cccttgaaat ctcttccatg gagtgggagg cctggttgga aaccatcatg ggcacccccg
     2341 tctctttgca ggcgcgggct acggcgaggt ccccttccgg atgggccagt tccaggacgc
     2401 cgatggggct gaggaagaag ggcaggggat aggaatgccc gagtagtgtc cttgaaagtg
     2461 ttgcttctcc caccgatttc aacatgcgcg ggacgatggc atatttatcc caggctgagc
     2521 gattggcctg catggtaaac tcgaggccgg caccgcccgc gatgtaggca aatgcttctg
     2581 aggaaagctt ctgttttgcg gccagttcca gggaaggaaa atcaatagga ataaggggtt
     2641 tgatcccggc caccccatgg acataaattt ccctttgcct ggaaagggca ttaaaattca
     2701 tgactcaggt tttaggccct tcatccacga tatcggcgtc ttcgatgtga tcatccggtt
     2761 ttggggtcgt ggaattggac agcaggtcat tgagttgttt ctggtctttg gggtcgagcg
     2821 gtttttcttc cgccggtgca ggctccacct tttttacttc aacattgata ggcttttgtc
     2881 ctttcttgtc ctgatcgtcc aggaattttt tggctttgtc aaaaaagtcc tcgtgtttac
     2941 ccatcagcgg gtcggtgagg tttttgtttt ccgaaatggg tttttccccg aattcgcctt
     3001 tgggttcctt ggcttccagg gctttttctt tttctataaa ctcgtcaatt ttctgattga
     3061 tactccttgc ttgttcttcc accaccgtct tggctttttc atacagttcg gagccttttg
     3121 ttttggcttt atcggtgatg ttttcggaaa actgttccgc ttttttccag aaatcatccg
     3181 tggcttcgac caccttgccg cccagttctt tggcttcttc ctttaggttt gaggcccctt
     3241 ctgcgatctt ttctccggcc tggcccatgg ttttccggat gggttccaca aattcagagg
     3301 cgggttgttt ggcgggctcc tccggtttgt tttcaaaggg ggtttcgggt tttggggtgg
     3361 tatcccagag atcatccact ttctcgagga tctttgctcc ggtcttccct attttttcac
     3421 ctgcttcttt tacaaactcg gaagttttgt caaacaattc ttcccccttt tctttggtga
     3481 agtccaccgc tttttcagcc tggtgcttgg tgacgccttt gacgccgaaa agaagttttt
     3541 tgagaggatc gaggataccc atgatgagtg attttgtatt tccaaagtta agataaaaag
     3601 gttagaggtg cgaggttaga ggttagaggt aagaggtaag aggtcagagg tcagaggtta
     3661 gaggttagag gacataggtg agagataggg taacttaact taaaggatct gtaacgcccc
     3721 tgtcccccac gggggaaaca agcttctgaa tgcatcctaa attaaaaaca accggacttt
     3781 gcccttgtca gaataagtac agggataggt caattaaatt tattccgaaa tgtcgagggt
     3841 cgggttcccc ttcagggcct gccccaaatt taggatttga gaattagcag gtgggggaca
     3901 ggggtcggag tactacctat taaaaactcc aacgcccctg acccctaaag ggggaccaag
     3961 gatcctggca gaatacctag ataaaaacaa tcatccttgc ccttgtcaga ataagtacag
     4021 ggataggtca attaaattta ttccgaaatg ccaggggttg ggttcccctt taggggtcag
     4081 gggtcggaga actaatacta aaaggaactt cacatagagg atcttcaaca ctcagagatt
     4141 agaggtgcga ggtcagaggt tagagtgaca cccaccatcc accacccacc atccaccatt
     4201 caccgctttt gcatatacct actaatccca agatgcagca agccgcacaa caaccaggcc
     4261 ggcagagtca ggaaagaaag aaataccccc tgggataccg aaatggagta gaagatccca
     4321 aaactgagcc cccaggccag gagtacggcg aggttgaagt gaatgccttt cttttccgcg
     4381 taattggttt ctatatcggc tttacctgcg aaaaaatgct cgaagactat gacggccccg
     4441 acgggagcga ggataaaacc ataaagagct acaaaaccca ggagtttcat ggcaaaggcg
     4501 gggaagatcc cggcgatggt ggcgatggcg ccggcgagga tggtcaccca aaaggtggac
     4561 gccctgggga tgatcgcctg gaaggccagg cctgcccggt agatagtagg attggcggtg
     4621 gtccagccgg cgagaatgac ggtgagggag ccgatcacgc caagtgcatt ataggccagg
     4681 ggcccaggag caacgggtgg cgcgacgccg gcagaaagca tgccttgggc ctccggtgac
     4741 ttgagataga cggcatacag gagcgccgca gcgatccagg ccaggtaatg tcctacatac
     4801 atgcccgccg ccgtagtcca gcctacgctg gcttttttgg caaagcgaag tacggagagg
     4861 tcggacatgc cgatatgcat ggccgcattg caaaaccagg accataaggt aacgtgccag
     4921 aaggtatatt ttatttggcc gggaaagggc tctgagccgg tgccccagat ggcccagaaa
     4981 tcggaaaagt ttttgacccc cagctggccc agggcgacta tgccgcaggc cataaaagca
     5041 atgacgataa agggggccat ccagttggcg gctttggcca cagtattata tcctttggcg
     5101 gcgatgatgg acaccagggc gcccaggacc aggcaaataa tgacccaggt gaggctattg
     5161 ggcagggtat cttccagttt gggcatttcc atattgaaag gaatgcccac ggccgtggcc
     5221 gatacggtga tcatggcccc ggcaaggaaa caaaacaaga ccccgttggc gatattgtaa
     5281 aaggtcacca ggtttttacc gcagattttt tccagttgat aatagagcgt aagcctgttt
     5341 ttcactgcaa tctcagccgt caggaatctc cagcaaatga cggccataaa attgccgagc
     5401 aacaagccga cgatgaggtc gaaagcactc acccctgagg tcaggaataa aggcccgatc
     5461 ataaactccg ttcccgccac atgttccccg gcatacatgc ccaggaaact tttccagtcc
     5521 ttccagcggg aggggggaac gggctggcgt tcaaattcat cggtgtgaat gatttcctct
     5581 gcgatgctat ccatgttttt acgatttcat tttgggatca ggtgccggta aagttcggaa
     5641 ggatgggtgc aacagacctg ttccaggatt tgtttgagct ggattttcag aatcgagatg
     5701 gtatggttcc ctccttcatt gcccagggct gccaccccat acataaaggt cctgcccagg
     5761 aatacaaaat cggcgccgga ggccaggacc cgggcgatat caggccccgt gcgcaccccg
     5821 ctgtcgagca tgatcttgat tttgcctttg tacttctttt gcagggcgac cagggaatgc
     5881 agggtggatt ccccggcatc cagttgccgg cctccgtgat ttgagatgat gatgccgtcg
     5941 acgccgagcc ggatggcggt ttcggtatct tcttcactgg ccaccccttt gatcaccagt
     6001 ttacctttcc agcggtcccg gatcgggccg atgcgttctt cgctgagtcg accggaaaaa
     6061 gtcatattca taaactgccc gagttgtttc atattgaggc cgccgggcat atattgtttc
     6121 atggttgcga aattaggcgc cccatgatac agggtccgca gcgcccagtt cggtttaccc
     6181 aggatctgga agatattcct cagggtcatc cggggtggga gggcgagtcc gtttttgata
     6241 tccctcggcc ggaaggcaaa ggtgggtaca tcactcaaca acactaatac aggcagttgg
     6301 gccgcttcaa cccggcgaag gatatccgtc ctcacctcct ccgcgcttgg ataataaagt
     6361 tgaaaccagg cctttcctcc ggtgatctcc gcgatccgtt cgatgctgga ggtgctcacc
     6421 gtgctcaggg tgaatggaat attatgttcg gtcgccgccc gggccaggat ttcaggactg
     6481 ttgggccata ccagtccctg gaggccgatg ggggagatcc caaagggggc gtcatagacc
     6541 tggccgaaga gttctgtttt caggtcggat ggggtatagg cgcccaagta gtaaggtttg
     6601 agctccactt cccggatatc cgaggtattt tttccgaggt tgacgtcttc gttgcatccg
     6661 ccgtcgaggt attcaaaagc gaagcggggg atgcgtcttc tggccgcatt gcgcaaatca
     6721 tcaatgccgg ggtagttagt attgtaagac caggtcatat cggtttttta ggacattccc
     6781 gataaaggcg ctggcccttt aagggaatgg gctgggaaaa tattaaaatt ttttggattg
     6841 gaaggtctgg gaaatgattt accgggggac tttatctacg gttgctcgag gtcgtcctcg
     6901 cctggagcgg gggtttattt gaatgtggag ccaaagatct ttacggtata cctataattg
     6961 attataaata tctttaacgc ccctgtcccc tgaaggggca ccaaggatcc tggcagtata
     7021 tcaaataaaa acagtcatcc ttgcccttgg taaggagtac agagatgggt cagaaatttt
     7081 atataaattg tttttcatcg tccctgcccc ccctgactta cttgactgag tacagagatt
     7141 ttttgaacgc ccctgtcccc taaaggggga accatggatc ctggcagtat gaggtcagag
     7201 gtcagaggtt agaggtcaga ggtgaggact tcatataaaa tatcttcaaa gcctctgatt
     7261 cccctgaagt atttaattga gtatatagat ttctttaacg cccctgtccc ctaaagggga
     7321 accaaggatc ctgacagtat atctaaatta aaacaattgt cctttccctt gtcggaatga
     7381 atacaggggt aggtcaatta aattattccg aaatgccaga ggtctggttc cccttgaggg
     7441 gtcaggggtc ggagaataat ccaaaaagga atcttcatat taatatttcc aacgcccctg
     7501 tcccccctga ctaccaattc tcaaatgcag tccggggcag gcccgcctga ccaccttttc
     7561 tcaaaaaata ctggtcgggc aggcctgaag ggggaccaag gatcctggca gtaagaggtc
     7621 agaggtcaga ggtcagaggt gaggacttca cataaaatat cttcaacgcc tctgatcccc
     7681 catatcttca acgcctctgc ctcccctgac ttccttgatt gagtacagag atttcttcaa
     7741 cgcccctgcc ccccctgact tctaattctc aaatgccttc tgggggcagg cctgaagggg
     7801 aacgaaggat cctggcagtg tactcaaata aacaatcttt cttgccctta ccggaataag
     7861 cacagagatg gatcaaataa aatttatttc ggatgccaag ggtctggttc cccttgaggg
     7921 gtcaggggtc ggagaactaa tactaaaagg aacttcatat taatatttcc aacgcccctg
     7981 tcccctgaag gggcaccaag gatcatggca gtatgcctaa attaaaacaa tcttccctgc
     8041 ttttggtaag gagtacagag atgggtcaga aattttatag aaaatgtttt caacgcccct
     8101 gtcccccctg acttacttga ttgagtacag agatttcttt aacgcccctg tcccccctga
     8161 cttcaaattc tcaactgcct tctgggggca ggccccctta ctaccaattc tcaaatgctg
     8221 cccggggcag gccccctgaa acagccccct aaattttcag acatagttac aaaaaaattt
     8281 tagtaat
//
LOCUS       DAOQXT010000157         8277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_9120_length_8277_cov_6.271224, whole genome shotgun sequence.
ACCESSION   DAOQXT010000157 DAOQXT010000000
VERSION     DAOQXT010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9120_length_8277_cov_6.271224"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKM27_18095"
     CDS             complement(<1..532)
                     /locus_tag="PKM27_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09237.1"
                     /translation="MDFRRLVLWGVLIGTAACKHEPEYKGPGRVTEAEIEGEQGEQSG
                     AGLALEAWYQARFADPGQVNPTSLMPAYEKLKQHTRSPDKANWRSIGPKNIGGRTLCL
                     AFHPTDPNVIYAGSASGGLWKTSSSGIGYHGWVQVPIDLPVYSVASILISPRNPNLMF
                     IGTGEVYNKAAAAPGIV"
     gene            670..4023
                     /locus_tag="PKM27_18100"
     CDS             670..4023
                     /locus_tag="PKM27_18100"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNR09238.1"
                     /translation="MIEGKESIATLIYQELEDIQQQGFEEGAKSITRLYQALNYFFNR
                     VTANDKIYFSTLFARMVYAGIQYRIPRRDLSLLHHFRKISYQQIYHTQAPGKLSTEQL
                     LKLGIYSLNRVVQYLCETASPLVFMGWEQKSWESLFEEPRFDKTIANCRVLITHADPE
                     EQELTGVSEEYPHHAVRIRYHVSGINDTQTELLQAYLQLRPLPVSIQLIDIEVEAAGA
                     WIPHQMVFEPDYLVDVTAVAECFKDFGVLTWSYLLNKYKPKPSSPSILLGNAANFFLD
                     EIMVNPDADFPALMKQFFKLYPLECTVLEDREVIDLNVKAKTHFNNLRRVTREEFSQI
                     GIQAEGVFLEPTFISAIYGLQGRLDLYHETPERNLAHIVELKSSKPYKTNAYGINPNN
                     YIQTLLYDLLIRSVFKNKTRLSSYILYSQQEQKPLRFAPPVKAQQIEALAVRNELVIL
                     ELRLSRSEELDHLLGQLRPSLLPTPQGFIANDLQGFESAYQAMSGLEKAYLREFTGFI
                     AREHHLAKVGEHSDESNNGMASLWLNNRLEKEDNFAILSHLVFKSAEVRGDDEFLTFE
                     RTSESNELANFRQGDIGVLYPHTWSGASALEHQIFKCSIIDIRANQLMIRLRNKQRRK
                     NIFSEWKYWNLEHDLIDSSFHILYRNLFEFLTGDEEKRQLLFNLRSPGLPEEGAGEIS
                     LPADLNDEQKTILQKILRTRDYFLLWGPPGTGKTSIMLRSLSQHLYTNTQESILLLAY
                     TNRAVDEICEALESASLDFLRIGARHTAQKKFWKHFLDEQASGLERRSEFKHLIENHR
                     IITGTLTSINGKPELFQLKKFDWVIVDEASQILEPMLAGILPRVKKWVLIGDHRQLPA
                     VVVQRKDDSAVQSPELKEIGLINLRNSLFERMMNACKTNQWTWAFDILREQGRMHRSI
                     MQFPSRYFYDDALRVLQQPDIRLELEQPLGYKTDPAWKGLIQDLARKRMLYLPSPADN
                     QGKSKINRMEAGLVSDCIEAIRRIYAFNGLDLHAQSIGVITPYRAQIAQIRKTLQDHD
                     LDPDLLTIDTVERYQGGARDIIIFSLCTNRFSQIQNMISLSDDGVDRKLNVALTRARR
                     QMIIIGNEEILREYPVYKDLIEYCNQEGI"
     assembly_gap    4336..4435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4775..5377)
                     /locus_tag="PKM27_18105"
     CDS             complement(4775..5377)
                     /locus_tag="PKM27_18105"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HNR09239.1"
                     /translation="MNFSMNNDKNNKNDLAQEIVKTHLLLAVGGGLIPVPLVDFAAVT
                     GVQINMIRSLAKLYGVDFNEQVGKSIVSSLVGTSLARLAASAVKAIPLIGSILGAASM
                     SVLSGASTYAVGQVFIQHFGKGGTLSDFNAEQAKSKYQEEFERGKEFSVRAEKEQKSS
                     SSQSREEIFAALERLNDLKNRGVLSEEEYNSQKEKLLGRI"
     gene            complement(5554..6180)
                     /gene="queE"
                     /locus_tag="PKM27_18110"
     CDS             complement(5554..6180)
                     /gene="queE"
                     /locus_tag="PKM27_18110"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HNR09240.1"
                     /translation="MYLVKEIFYTLQGEGVHTGRPAVFLRFAGCNLWNGLESHRASAI
                     CKFCDTDFVGLDGLHGGKYDAEGLVGVIRSLWPDPEIPIYLVCTGGEPALQMDQALVD
                     AFHREGIIIAIETNGTLPLPEGIDWVCVSPKSDTALAITRGNELKLVYPQEEALPERF
                     EFLDFDHFSLQPMDGPLQKINTAKCLEYCRNHPKWKLSLQTHKILEIP"
     gene            complement(6189..6602)
                     /locus_tag="PKM27_18115"
     CDS             complement(6189..6602)
                     /locus_tag="PKM27_18115"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNR09241.1"
                     /translation="MNRLHISSGAKWEDLVGYSRAIRIGDLIEVSGTTAMKDGKLVGK
                     DNLYLQTRTCLEIIESALQRAGSSMHDVIRTRIYVTDITRWEEAGKAHGEFFGKIKPA
                     TAMVQVAALIDPDMLVEIEATALVSQGNADPGTGV"
     gene            complement(6599..7144)
                     /locus_tag="PKM27_18120"
     CDS             complement(6599..7144)
                     /locus_tag="PKM27_18120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR09242.1"
                     /translation="MTKKKKTGKALFLDRDGVINRRLPGDYVKTWKEFDFLPGTLRAL
                     GLLRPYFDRILVVTNQQGIGKGKMTEADLANIHRNMKKVIRKSGGHIDRVYHCPDLES
                     RQANCRKPNPDMGKWAKADFPEIDFDRSVMVGDTDMDLLFGKNLGMTTILIAASPAGS
                     KDLAEHSFRDLMDFYLNFVKK"
     gene            complement(7141..7833)
                     /locus_tag="PKM27_18125"
     CDS             complement(7141..7833)
                     /locus_tag="PKM27_18125"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNR09243.1"
                     /translation="MIAIVLAGGLGTRLRPVTGDLIPKALVPVQGKPFLHWQLNYLKK
                     QGIKKIILAVSFHAGQVIEYFGTDFQGLPLEYSVEKKALGTGGATKRAMTLTGNKPVF
                     VCNGDTWFKVNLEKMRQKHRLAGHAVTLALKKMNRFDRYGSVSLGSKDRIVHFEEKKW
                     LETGYINGGIYYFNPGLLRDYPPDTAFSLEKDFFEKMVKEIKIGGYRSRASFIDIGIP
                     EDYRKIQSLDLR"
     gene            complement(7833..>8277)
                     /locus_tag="PKM27_18130"
     CDS             complement(7833..>8277)
                     /locus_tag="PKM27_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNR09244.1"
                     /translation="KILFCGNGGSAADAQHLAAEFSGRFYKDRPPLYSEALHVNTSYL
                     TAVGNDYGYDQVYSRMIKGIGKKGDILIGLSTSGNSQNIIHAFEAAHALGMICIGFTG
                     ATGGKMAVLSDLLINVPSTDTPRIQESHITIGHILCELVEKSLFP"
BASE COUNT         1957 a         2125 c         2075 g         2020 t
ORIGIN      
        1 cgacaatgcc tggagctgcc gccgctttgt tatatacttc accggtcccg ataaacatga
       61 ggttggggtt ccgggggctg atgaggatgg aagccacgga atacacggga agatcaatcg
      121 gaacctggac ccagccgtgg taaccgatgc ctgaactgct ggtcttccac aaacctccac
      181 tggcggaccc tgcgtatatg acgttgggat ccgttggatg gaaagccagg cagagggtcc
      241 ggcccccgat attttttggc ccgatcgacc gccagtttgc cttgtcggga ctgcgagtgt
      301 gttgttttaa tttttcataa gcgggcatca ggctggtagg attgacctgt cccgggtcgg
      361 caaaccgggc ttggtaccag gcctcaagcg cgaggccggc accgctttgt tcaccttgtt
      421 ctccttcaat ttcagcttca gtaacgcggc cgggaccttt atattccggt tcgtgtttgc
      481 aagccgcagt ccctatcaaa actccccaca aaaccagcct tctgaagtcc atgagatatt
      541 aaagtaattc ttaatataaa tcgcaaatat aataaatcga ttatacctga aaaacccctt
      601 gtgatttctt aacaacatat tccgtgccat ccgtggacaa atccttcaaa tgccctattt
      661 ttacttcgaa tgattgaagg aaaagagtcg attgcgacct tgatctatca ggaacttgag
      721 gacatccagc agcaggggtt tgaagagggc gccaaatcca taacccggct gtatcaggcc
      781 ctgaattatt ttttcaaccg ggtcacggcc aacgacaaaa tttatttttc caccctcttc
      841 gcgaggatgg tttatgccgg gatccagtac aggattccga ggcgggacct ttccttgctg
      901 catcatttca ggaagatttc ctaccagcag atttatcata cccaggcccc gggcaaactg
      961 agcacggaac aattgcttaa actcggtatc tatagcctaa accgggtggt gcaatacctc
     1021 tgtgaaaccg ccagccccct ggtcttcatg ggctgggaac aaaaaagctg ggaatccttg
     1081 tttgaagagc cccggtttga caaaaccatt gccaactgcc gggtattgat cacccatgcc
     1141 gatccggagg agcaggagct tacgggggtt agtgaagaat acccccatca cgcggtccgt
     1201 atacggtatc atgtatccgg gatcaacgac actcaaaccg agttgcttca ggcttacctc
     1261 caattgcggc ctttgccggt ttccatccag ttgatcgaca ttgaggtgga agccgcaggc
     1321 gcctggatcc cccaccagat ggtttttgag ccggattacc tggtggacgt gaccgccgtt
     1381 gccgaatgtt ttaaggattt cggcgtgctg acctggtcct atcttctcaa caaatacaaa
     1441 cccaaaccat ccagtccgag tatcctgctg ggaaatgccg ccaacttttt tcttgatgaa
     1501 atcatggtga accccgacgc ggatttcccc gctctcatga aacaattttt caagctctat
     1561 cccctggaat gcacggtcct ggaagaccgg gaggtgatcg acctgaatgt caaagccaag
     1621 acccacttca ataacctgcg gcgggtcacc cgggaagaat tcagccagat cggcattcag
     1681 gcggaagggg tattcctgga acccactttc atttcagcca tttacggatt gcagggcagg
     1741 ctggatcttt accatgaaac cccggaacgg aacctggccc atatcgtgga actgaaaagt
     1801 tccaaaccct acaagaccaa tgcctatggg atcaatccca ataattatat ccagacctta
     1861 ttgtacgact tgctcatccg gagcgtgttt aaaaacaaaa cccgcctttc ctcctatatt
     1921 ctctattccc agcaggaaca gaaacctttg cgattcgctc cgccggtcaa ggcccagcag
     1981 atcgaagcgc ttgcggtccg caacgaactg gtgatcctgg aactccggct cagccgcagc
     2041 gaggaactgg atcacctgct cggacaattg aggccttccc tgcttcccac cccccaggga
     2101 tttatcgcca atgatcttca gggttttgaa tcggcctacc aggccatgtc cgggctggaa
     2161 aaagcctatc tcagggaatt tactggcttc attgcccgcg aacaccattt ggccaaggtg
     2221 ggggagcaca gcgatgaatc aaacaacggc atggcttccc tctggctcaa caaccggctg
     2281 gagaaggaag ataattttgc cattctcagc cacctggtat tcaagtccgc cgaggtccgc
     2341 ggggacgacg agtttttgac ctttgaacgc acatcggaat ccaatgaact ggccaatttc
     2401 aggcaggggg atatcggagt attgtatccg catacctggt cgggggcatc cgccctggaa
     2461 caccagatct tcaaatgttc catcatcgac atccgggcta accagctcat gatccggctg
     2521 aggaataaac aacgccggaa aaatattttt tcggaatgga aatactggaa cctggagcac
     2581 gacctgatcg acagcagttt ccatatttta taccgcaacc tctttgaatt cctcaccggg
     2641 gatgaagaaa aaaggcaact cctgttcaac ctgcgctcgc cgggcttacc tgaagagggg
     2701 gcgggggaaa tatcattgcc tgctgacctg aacgatgaac aaaagactat tctgcaaaag
     2761 atcctgagga cccgtgacta tttcctgctt tggggcccgc cgggtacggg caagaccagt
     2821 atcatgctgc ggtcgctgag ccagcacctt tacaccaata cgcaggaatc catcctcttg
     2881 ctggcctata ccaaccgtgc cgtggatgaa atttgcgaag cgctggaatc cgcttccctg
     2941 gattttctgc ggataggggc caggcatacg gcccagaaaa agttctggaa acattttctg
     3001 gacgagcagg ccagcggcct ggagaggagg tcggagttta aacatttgat tgaaaatcat
     3061 cgcatcatca ccgggaccct gaccagcatc aacggcaagc cggagctttt tcagcttaaa
     3121 aaattcgact gggtcatcgt ggatgaagcc tcccagatcc tggagcccat gctggcaggc
     3181 atcctgccca gggtaaaaaa atgggtgctg atcggcgacc acaggcaatt gcccgcggta
     3241 gtggtccagc gtaaggacga cagcgcggtc cagagtcccg agctgaagga aatcggcctg
     3301 atcaacctgc gcaattccct cttcgaacgg atgatgaatg cctgcaaaac aaatcagtgg
     3361 acctgggcct ttgatattct ccgtgaacaa ggacggatgc acaggtccat catgcagttt
     3421 cccagccggt atttttatga tgacgccctg cgggttcttc aacagccgga tatccgcctg
     3481 gaactcgaac agccccttgg atataaaacc gatcctgcct ggaaaggact catccaggac
     3541 cttgcccgga aaagaatgtt gtacctgccc tccccggcgg acaaccaggg taaatcaaaa
     3601 attaaccgga tggaggcggg cctggtcagc gattgtattg aagccatccg ccggatctat
     3661 gccttcaacg gcctggacct tcacgcgcag tccatcggcg tgattacccc ttaccgggcg
     3721 cagattgcgc agattcggaa gaccctgcag gaccatgacc tggatccgga cctcctgacc
     3781 atcgataccg tggagcgtta ccagggcggg gccagggata tcattatctt ttcattgtgt
     3841 accaaccggt ttagccagat ccagaacatg atctccttgt cggatgacgg ggtggaccgg
     3901 aaactaaatg ttgccctcac gagggccagg cgccagatga tcatcatcgg gaacgaagaa
     3961 atactcaggg agtacccggt gtacaaggac ctgattgaat attgtaacca ggaagggatc
     4021 tgaccaaaaa gtccggataa agttgttgta atgtgcccgg cgcataaagg gttgtttcag
     4081 agctaccgtt ccgaaaactc ggaatctcgg tattcacctg aaacggaaaa gtaaaatgct
     4141 agaagttgaa agtatcgtat tacctcaggc atcctgctaa aaagcttcag gcgggacgca
     4201 ctgaagcaac cacgcttttg tttggcagta tactggggtt ttagcaaaat ggtagaaatt
     4261 gaaagtactg gcctacctag ggcatcctgc taaaaagctt caggcgggac gcactgaagc
     4321 aaccacgctt ttgttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggta
     4441 gtatactgtg gttttagaaa aatggtagaa attgaaagta ctggcctact tagagcattc
     4501 tgctaaaaaa cttcaggcga gacgctctga agcaactaag ctggtaaacc actttgggct
     4561 gactaaaaat tccagttgtt gtttatgttc ctgggcgcat aaggggttgt ttcaggcgtc
     4621 ctcgcctgaa acgggaaagc agaatgttat atattggaag tactggttta ccaaggcatt
     4681 ctgctaaaaa gctacaggcg agacgcactg aagcaaccac gcttttgttt ggcgatatcc
     4741 attctgctaa aaaactagca aaatgttctc tatctcagat cctgcccagc agtttttcct
     4801 tctggctgtt gtattcttct tcggagagga ctccccggtt tttgagatcg ttcagtcttt
     4861 caagggcggc aaagatttcc tccctggact gggacgaact tgatttttgt tctttttcgg
     4921 cgcggacgct gaattctttt cctctttcaa actcctcctg gtatttggat tttgcttgtt
     4981 cggcgttgaa atcggaaagg gtgcctccct taccaaaatg ctgaataaac acttgtccga
     5041 ccgcataggt gcttgcaccc gacaagaccg acatggaagc cgcgccaagg atggaaccga
     5101 tcaggggaat ggctttcacc gcggaggccg ccagtctggc cagtgaagtc cctaccagcg
     5161 aagagacgat gcttttgccc acttgttcat taaaatcgac cccatagagt tttgccaggg
     5221 agcggatcat attgatttgt accccggtga cggctgcaaa atccaccagg ggtacgggga
     5281 taaggcctcc ccctaccgca agtaataaat gggtttttac tatttcctgg gccaggtcat
     5341 tcttattgtt tttgtcgttg ttcatgctga aattcatttt tttactaaaa ttataggcct
     5401 gctttccgcc tttgaataaa attcgaccaa tatccgtgta tatcgacatg taagtagtac
     5461 aaattctgtc taaacttagt tccggttttg ggcatcctta ccgggtggta taggatcaat
     5521 gagcctttca agcctcccgg gtcactcttt caatcagggg atctccaaaa tcttatgggt
     5581 ctgaaggctg agtttccatt ttggatgatt cctgcagtat tccaggcatt ttgcggtgtt
     5641 gatcttctgc agagggccgt ccatgggttg caggctgaaa tggtcaaaat ccaggaattc
     5701 aaatctttcc ggtagggcct cttcctgggg ataaaccagt ttcagttcat tgcccctggt
     5761 gatggcgagg gccgtatccg atttggggct cacacaaacc cagtctatgc cttccggaag
     5821 gggcagggtc ccattggttt ctattgcaat gatgatcccc tcgcggtgaa aggcatccac
     5881 cagggcctgg tccatttgta atgccggttc ccctcccgta cagaccaggt aaatggggat
     5941 ttcaggatcc ggccaaagcg aacggattac accgactagg ccttccgcgt cgtattttcc
     6001 tccgtgaagc ccgtccaggc ccacaaaatc cgtatcacag aatttacata tggcggaggc
     6061 gcggtgcgat tcgagtccat tccaaaggtt acatccggca aaacgcagga acaccgcggg
     6121 gcggccggta tgcacgcctt caccctgcag ggtataaaat atttctttta ccaggtacat
     6181 ggatagatct aaacaccggt gcccgggtcc gcgttcccct gactgaccag ggcggtagct
     6241 tcaatttcca ccaacatatc cggatctatc agggcagcca cctggaccat ggccgtggcc
     6301 ggttttattt ttccgaaaaa ctctccgtgg gccttgcccg cttcttccca ccgggtgatg
     6361 tccgtgacat agatgcgtgt ccggatcacg tcatgcatgg aagatccggc gcgttgcagg
     6421 gccgattcaa tgatttcaag acaggtcctg gtctgcaggt aaaggttatc cttgcccacc
     6481 agttttccat ccttcatggc cgtagtgccg gaaacttcga tcaggtcccc gatgcggatc
     6541 gcccggctgt agccgacaag gtcttcccat ttggcccctg aactgatgtg taaacgattc
     6601 atttttttac aaagttaagg taaaagtcca tgaggtcccg gaagctgtgt tcagccaggt
     6661 cctttgatcc tgcagggctt gcagcaatga gtatggtggt catgccgagg tttttcccaa
     6721 aaagaaggtc catgtcggtg tcgcccacca ttaccgagcg gtcaaaatcg atttcgggga
     6781 aatcggcctt ggcccatttc cccatgtccg gattgggctt cctgcagttg gcttgccggg
     6841 actccaggtc cggacaatga tagacacggt cgatatgtcc tcctgatttg cggatgactt
     6901 ttttcatatt ccggtggatg tttgcaaggt cggcttcggt catcttcccc ttgccgatgc
     6961 cctgctggtt ggtgaccacc aggatccggt caaaataagg gcgcaggagg cctaaagccc
     7021 ggagcgtccc gggcaggaag tcaaattctt tccatgtttt tacatagtcg ccgggcagcc
     7081 tccggttgat caccccgtcc cggtccagaa acaaggcctt gcccgttttt ttctttttgg
     7141 tcatcgaagg tcgagggatt gaatcttgcg gtaatcttca ggaatgccga tatcgataaa
     7201 ggaagcgcgt gaacggtatc ccccgatttt tatttccttc accatttttt caaaaaagtc
     7261 cttttccagg gagaaggcgg tatccggggg ataatcccgg agcaggcccg gattgaaata
     7321 atagatccct ccgttgatat aaccggtttc gagccatttt ttttcttcaa aatgaacgat
     7381 tcggtctttt gatcccaggc tcaccgaacc gtaccggtca aagcggttca tctttttcag
     7441 ggcgagggtg accgcatggc ccgcgagtcg gtgcttttgt ctcatctttt cgaggtttac
     7501 cttgaaccag gtgtccccgt tgcatacaaa taccggtttg tttccggtga gcgtcatagc
     7561 ccgcttagta gctcctccgg ttcccagggc ttttttttcc acggagtatt ccagcggaag
     7621 tccctggaaa tcggtaccaa aatactcgat gacctggccg gcatggaaag aaacggccag
     7681 gatgattttt ttgattccct gttttttcaa ataattcaac tgccagtgca ggaaaggttt
     7741 gccctgcacg gggaccaggg cctttgggat cagatccccg gtgaccgggc gaagccgggt
     7801 gcccagtccg cctgcaagga caatggcgat catcagggaa acaaggattt ttcgaccagt
     7861 tcacaaagga tatgtccgat ggtgatatgg ctttcctgta tgcggggagt atccgtggag
     7921 ggcacgttga ttaaaaggtc gctcaggact gccatctttc ccccggtggc ccctgtgaaa
     7981 ccaatgcaga tcatgccaag ggcatgggcc gcctcaaaag catggattat attctgggaa
     8041 ttgcctgagg tgctcagccc gatgaggata tctcctttct tcccgatccc ttttatcatc
     8101 ctggaataca cctgatcata accgtagtcg ttcccaaccg cggtgagata ggaagtattg
     8161 acatgaaggg cttccgaata aaggggcggg cggtctttat aaaatcggcc ggaaaattca
     8221 gcggccaggt gctgggcatc cgccgcactg cctccattgc cgcaaaacag gattttg
//
LOCUS       DAOQXT010000158         8107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_9351_length_8107_cov_6.267014, whole genome shotgun sequence.
ACCESSION   DAOQXT010000158 DAOQXT010000000
VERSION     DAOQXT010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9351_length_8107_cov_6.267014"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="PKM27_18135"
     CDS             complement(<1..2270)
                     /locus_tag="PKM27_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNR09245.1"
                     /translation="MKKFFTYILFAGFALVSFRYTTPKYSQQQNGPFTLVQNKGGATL
                     GYSPASGVKIITVDRLAFKDLNRNGKLDPYEDWRLTADLRAKDLASRMSVEQIAGLML
                     YSGHQAIPGGGMRGGSTYEGKAFDPKVHVASALTDQQKEFFEKDHLRHVLLTRVQSPQ
                     IAAEWNNNAQAFVEGLGLGIPANNSSDPRHGTTSDAEYNAGAGGNISMWPGSLGLAAS
                     FDPALVKKFGDIASREYRALGIATALSPQIDLGTEPRWNRFRGTFGESPQLAADLARA
                     YVDGFQSTRGKGWGMESVNAMVKHWPGGGPEEGGRDGHYGYGAYAVYPGNNLKDHIHV
                     FREGAFKLNDGTGSASAVMPYYTISYGQGHQGENIANAYSSYIIQDLLRTKYGYDGVV
                     CTDWGVTGIVTTVDEFRGKPWGVEHLSEAERHYKVLMAGCDQFGGNNVAGPVIEAYQM
                     GVKEHGEQKMRARFEQSAVRLLKNIFRVGLFENPYLDVALSKTTVGNPEFMKAGYEAQ
                     LKSLVLLKNKNKTLPLAKGKKVYIPQRYVPESRGFFGPPTPAKWVTPAKKEIVEKYFQ
                     ITDDPAMADFALVFAESPKSGVGYDKKDKEKGGNGYLPISLQYQPYTATMARQKSLAG
                     GSPFESSADRSFYGKSVETANVKDLEVLLDTRKKMGNKPVVLSINLANPAVMAELEPA
                     ADAILVDFEAQHQAIFEVLSGNHQPSALLPFRLPADMSTVETQSEDLPFDMKVYRDGE
                     GHAYDFGFGMNWAGVIS"
     gene            2496..2750
                     /locus_tag="PKM27_18140"
     CDS             2496..2750
                     /locus_tag="PKM27_18140"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNR09246.1"
                     /translation="MLTTTISEFRKDIKKYLDQVTEGYDTLILNRGKDSGVVIMSLEE
                     YNSLTATHHELSSKKNEMRLDSAIEKLKKKDSFERELLEP"
     gene            2747..3004
                     /locus_tag="PKM27_18145"
     CDS             2747..3004
                     /locus_tag="PKM27_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNR09247.1"
                     /translation="MKFVFVAESWEDYLYWQKTDKKMMFRINLLLKDISKNPFSGLGK
                     PELLKYKYKGFWSRRIDGEHRLIYQVNGDEILIAKCRFHYD"
     gene            3020..3565
                     /locus_tag="PKM27_18150"
     CDS             3020..3565
                     /locus_tag="PKM27_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09248.1"
                     /translation="MNLSSIESKNYIEAHLDFLYYVGKRTGILKNSMNFTAFQNLGLK
                     VKFQCRDRFLKNPKLLDDYLTLYFNDLEPEKIKILEGFRKRISGDFVILKYLKNHAVF
                     IDLTTSKIYLVKALNDRLDQMIPGTPKMVKATILPFMDKIIYDGFLEPYTIIIGKGIK
                     NDLSHLYKKAKEKKELISEIK"
     gene            3579..6038
                     /locus_tag="PKM27_18155"
     CDS             3579..6038
                     /locus_tag="PKM27_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNR09249.1"
                     /translation="MRFYLFTISLLLVFDCTAQQNKALVLWYRQPSGEKWENALPIGN
                     GRLGAMVYGNPEEEIIGLNEHTVWSGGPNRNDNPLALASLPEIRALIFQGKHKEAETL
                     ANRTIISKKSHGQMFQPVGNLHLSFPGHKNVGQYYLELDIEKAVATTRYSVDGVEYSR
                     EAFASFADRVIVLKLSSGKPGSLSFTARYSSPQMNKKYSVSGGKDLEITGVTSDHEGV
                     AGQIAFKGLCRIKLNGGFMTQTDTSLLVQGANTATIFISIATNFNSYKDLGGDEKKRA
                     LVYLDKASGKPYETLKKAHITAYQKYFNRVHLDLGVTEAASLPTDERLKHFATANDPQ
                     LVALYYQFGRYLLISSSQAGGQPANLQGIWNHRINPPWDSKYTININTEMNYWPAEKT
                     NLSEMHEPLLQMVKELALTGQETARVMYGARGWMAHHNTDIWRATGAVDAAFWGMWTS
                     GGAWLSQHLWERYLYTGDKKFLTAAYPALKGAALFYVDFLIEYPGKNWLVVSPGNSPE
                     NAPPAHQGSSLDAGVTMDNQIVFDVFNTAIRAAEILKTDEAFRDTLRQMRSKLPPMHV
                     GRHNQLQEWLDDIDDPNDRHRHVSHLYGLFPSNQISPYRTPELFAAARTTLLHRGDVS
                     TGWSMGWKVNWWAKMQDGNHAYQLIRSQLSPLSGEQGSGGTYNNLFDAHPPFQIDGNF
                     GCTSGITEMFLQSADGAVHLLPALPEAWPSGSAGGLKARGGFEIVNMEWKDYQLVKLV
                     VKSSLGGNLRLRLPNEMKTGNGKALNAAVGKNSNPFYYLEETAPAILSEKANLKLLEL
                     KPTLLVDIPTKKGKVYTFSGH"
     gene            6097..7044
                     /locus_tag="PKM27_18160"
     CDS             6097..7044
                     /locus_tag="PKM27_18160"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNR09250.1"
                     /translation="MNRLQRLSAILIQLQSGHRVRAGDMAERFGVSLRTVYRDIQSLE
                     ATGVPVIGEAGSGYRLVDGYRLPPVHFTRGEIHALLTAQKLIEKQSDQGLDADFKAAL
                     FKIKAILRNPEKDLLSSLEENIEVLHNPYLPAPDRGNPFLPKLIQSISDKTRVYLDYT
                     NRQDVKSSRRETEPIGLFYLLGKWHLVAYCHLRKDYRDFRLDRIRELGLTSKPFAKRH
                     PTLKKYLSQLTREEKLNTIEIRMNKEDVHYLGEQKYYNGYVSERPDGDQVTLTFLTSS
                     LTGFAHWYMMIGTRAEILKPEILKEQVHRMATELLKNIK"
     gene            7108..7602
                     /locus_tag="PKM27_18165"
     CDS             7108..7602
                     /locus_tag="PKM27_18165"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR09251.1"
                     /translation="MISFIESFKKELEQEAVQTRRMLQIVPADKMDWRPHPKSMTIKA
                     LASHLAEIPLMITMALDHDKWDFANHPYAPVDMNNATELVNRFEQCLDEARASLNRAT
                     DDILQKKWVMCAGDAIYMTCEKWEAVRHAFGQNAHHRAQLGVFLRLLDIPIPGPYGPS
                     ADEM"
BASE COUNT         2127 a         2082 c         2020 g         1878 t
ORIGIN      
        1 gagatcactc cagcccagtt cattccaaaa ccaaaatcat aagcatgccc ctcgccgtcc
       61 cggtatactt tcatatcgaa gggcaggtct tccgattggg tctcgaccgt ggacatatca
      121 gcaggcagcc ggaacggcag aagtgcggag ggctggtggt tcccgctgag cacttcaaaa
      181 atggcctgat gctgcgcctc aaaatcgacc aggatggcat cggctgcagg ttcaagttcc
      241 gccattacgg ccggattcgc caggttgatg gagagcacta ccggtttgtt tcccattttt
      301 ttgcgggtat ccagcaagac ctccaggtct ttaacgttgg ccgtttctac ggacttcccg
      361 taaaaggagc ggtcggccga ggattcaaat ggacttcccc ccgccaggct tttttgcctc
      421 gccatggtgg ccgtataggg ttggtattgc aggctgatgg gcaggtaacc gttgccgcct
      481 ttttccttat cctttttatc ataacccact cctgacttcg ggctttctgc aaaaaccagg
      541 gcaaaatccg ccattgcagg atcatcggtt atctgaaaat atttttccac aatttccttt
      601 ttggcgggag tcacccattt ggcaggcgtc ggcgggccga aaaatcccct ggattccgga
      661 acatagcgtt gcgggatata gacttttttt ccttttgcca aaggcagggt tttgttttta
      721 ttcttcagca gcaccaggga tttgagctgg gcttcataac ccgctttcat aaattccgga
      781 tttccgaccg tggttttgga caatgccaca tcgaggtagg gattttcaaa taatcctacg
      841 cggaaaatat tcttcaagag gcgtaccgcg gactgttcaa aacgcgcacg cattttttgt
      901 tcaccgtgtt ctttcacgcc catttggtat gcttcgatga ccggaccggc tacattattt
      961 cctccaaact ggtcacaacc ggccatgagt accttgtagt gccgttccgc ttcggaaaga
     1021 tgttcgaccc cccagggttt gcccctgaac tcatccaccg tggtcacaat gcccgtgacc
     1081 ccccaatccg tgcacacgac cccgtcgtaa ccgtatttgg tcctcaagag gtcctggatg
     1141 atgtaagaac tgtaagcgtt cgcaatgttt tcaccttgat gtccctgtcc gtaggagatc
     1201 gtataataag gcattaccgc agaggcggaa cccgtgccat cgttcagttt gaaggctcct
     1261 tccctaaata catggatatg gtctttcagg ttgttgccgg gatagaccgc ataagcgccg
     1321 tatccataat ggccgtcacg ccccccttct tccgggcctc cacccggcca gtgtttgacc
     1381 atggcattga cgctttccat tccccatccc ttaccccggg tggactgaaa gccgtccaca
     1441 taggcgcgtg caaggtctgc ggccaattga gggctttctc caaaagtgcc cctaaatcta
     1501 ttccaacggg gttcggtgcc caggtcgatt tgcggcgaaa gtgccgtagc tattcccagc
     1561 gcgcggtatt cgcgggatgc gatatccccg aattttttga ccagggccgg atcgaaggaa
     1621 gcggcgaggc cgaggcttcc gggccacatc gagatattac cccccgcgcc tgcattgtat
     1681 tccgcgtcgc tggtggtacc atgacgggga tcggaactgt tgttggcagg gatgcccagc
     1741 cccagtcctt cgacaaaagc ctgggcatta ttgttccatt ccgcggcgat ttgcgggctt
     1801 tgtacccggg tcagcaggac atgcctgaga tggtctttct cgaaaaactc cttttgctgg
     1861 tcggtcaggg cggatgcgac atggaccttg ggatcaaaag ccttgccttc ataggtggag
     1921 ccgccccgca tacctcctcc agggatggcc tggtgcccgc tgtataacat caggcccgca
     1981 atttgttcga ccgacattct ggaagcaagg tctttggccc gcaagtccgc cgtcagcctc
     2041 cagtcctcgt aaggatccag ctttccgttt ctattgaggt ctttgaaagc cagccggtcc
     2101 accgtgatga ttttcacccc tgaggcgggt gaatacccca gggtcgcccc tcctttgttt
     2161 tgtaccaacg taaaagggcc gttttgttgc tggctatact tgggtgttgt ataccggaag
     2221 gaaaccaggg caaaaccggc gaagaggata tatgtaaaga attttttcat tgatacgatt
     2281 ttatccccta aaagtacagg attaaccggg ccgggattcc agcctgacgg atgatacgta
     2341 taatatttgg ggctatttgt agaactttac tggctccgga ccctaatcaa tgctttagcg
     2401 ccggactcaa gcaaaaacat tgaaaaaaaa ggaagaatca tctaaaaaaa tgtacacata
     2461 tttgtacata attaagtaca aaaaatattt attctatgct gaccacaacc atttcagaat
     2521 ttcgaaagga tataaaaaaa taccttgacc aagtcaccga gggctatgat accctgattt
     2581 taaaccgtgg gaaagacagt ggggttgtaa tcatgtcgct ggaggagtat aattcgttga
     2641 ccgccactca ccatgaattg tcctcgaaga agaatgaaat gaggcttgac tctgccattg
     2701 agaaactgaa aaaaaaggat tcttttgaac gggaattact cgaaccatga aatttgtctt
     2761 cgttgctgaa tcatgggaag attacctgta ctggcaaaag accgataaaa aaatgatgtt
     2821 cagaataaac cttcttctga aggatatttc aaaaaatcca ttttcaggac ttggaaaacc
     2881 tgagctccta aaatacaagt ataagggatt ctggtcccgg agaatagatg gagaacatcg
     2941 tttgatttac caagtgaatg gcgatgaaat tcttatagcc aaatgcaggt tccattacga
     3001 ttgaatatgc ctgggataga tgaacctttc cagtatagaa tctaagaact acattgaagc
     3061 ccatttggac ttcttatact acgtaggaaa gagaactggt atattgaaaa actccatgaa
     3121 cttcacggca tttcaaaatc ttggccttaa ggtgaagttt caatgcaggg atcgttttct
     3181 gaaaaatcca aaattactgg atgactacct gacactgtat tttaacgacc tggagcctga
     3241 gaaaataaaa attctggagg gatttagaaa aaggatatcg ggggattttg tcatcctgaa
     3301 atatcttaaa aaccatgccg tatttataga cctgactacc tcaaaaatat accttgtcaa
     3361 agctttgaac gaccgtcttg atcaaatgat tccggggacc ccaaagatgg taaaggcaac
     3421 cattctgccc ttcatggata aaataattta tgacgggttt cttgaacctt acactataat
     3481 aatcggaaaa ggtattaaaa acgacctaag ccatttgtac aaaaaagcga aagagaaaaa
     3541 ggaattgatc tccgaaataa agtaaagcaa aatcagccat gcgattctac ctttttacga
     3601 tttcccttct attagttttt gactgcacgg cccagcaaaa taaagccctg gtcctttggt
     3661 accgccagcc ttccggagaa aaatgggaaa acgccttgcc catcggcaac gggcgattgg
     3721 gggccatggt ttacggaaat ccggaggaag aaatcatcgg actgaatgaa catacggtct
     3781 ggagcggagg cccgaaccgg aacgacaatc ccctggccct ggcttccctg cccgaaatcc
     3841 gcgccttgat tttccaggga aaacacaagg aagcggagac cctggccaac cggaccatca
     3901 tcagcaaaaa atcacacgga cagatgtttc agccggtggg aaaccttcac ctttctttcc
     3961 ccggacataa aaacgtgggc cagtattacc ttgaactgga tatcgaaaaa gccgtcgcga
     4021 ccacccgtta ttcggtcgat ggggtcgagt atagccggga agcttttgcc tccttcgcgg
     4081 accgggtgat cgtgctaaaa ttgtcttccg gcaagcccgg cagcctttct tttacggccc
     4141 ggtacagctc ccctcaaatg aacaagaagt attcggtaag cggcgggaag gaccttgaaa
     4201 tcaccggtgt gaccagcgac catgaagggg tggccggtca aatcgctttc aaaggcctat
     4261 gccggataaa attgaatggc ggtttcatga cccaaaccga tacttcactc ctggtacaag
     4321 gcgcaaacac cgcaacgatt tttatctcca tcgccaccaa tttcaacagt tataaggacc
     4381 tgggcggcga tgaaaaaaaa cgggccctgg tttacctgga caaggcttcc ggcaaacctt
     4441 acgagaccct aaaaaaagcc catatcaccg cttaccaaaa atatttcaac cgggtacatc
     4501 ttgacctggg tgttacagag gcggcctccc tgccaacgga cgaaagatta aaacattttg
     4561 cgacagccaa tgaccctcag ctggtcgcgc tgtactatca attcggccgt tatctgctca
     4621 tttcctcttc gcaagccggc ggccaacctg caaacctgca gggcatctgg aaccatcgca
     4681 tcaacccgcc ctgggacagc aaatacacga tcaatatcaa tacggagatg aactattggc
     4741 cggccgaaaa aaccaacctc agcgaaatgc acgagccttt gttgcagatg gtcaaagaat
     4801 tggctctgac cgggcaggaa accgccaggg tcatgtacgg cgcccggggc tggatggccc
     4861 accacaacac cgatatctgg cgggccaccg gtgctgtgga cgcggctttc tggggcatgt
     4921 ggacgtcggg cggggcctgg ctcagccagc acctgtggga gcgatatctc tataccgggg
     4981 ataaaaaatt tttaacggcc gcttacccgg cattaaaggg cgcggccctt ttttatgttg
     5041 attttttaat agagtatccc gggaaaaact ggctggtggt gagcccgggc aattcgcctg
     5101 aaaacgcgcc ccccgcccac cagggatctt cgctcgacgc cggggtcacc atggacaatc
     5161 agatcgtctt tgacgtattc aatactgcca tccgtgcggc tgaaatcctg aagacagatg
     5221 aggccttcag ggataccctg agacaaatgc gttccaaact tcctccgatg catgtcggcc
     5281 ggcataatca actgcaggaa tggctcgatg atattgacga ccccaatgac cggcaccggc
     5341 atgtttctca tttatacggc ttgttcccat ccaaccaaat ctcgccttac cgcacaccgg
     5401 aactgttcgc ggccgcccgg accaccctcc tgcaccgcgg cgatgtatcc acgggctgga
     5461 gcatgggctg gaaagtcaac tggtgggcaa aaatgcagga cggcaatcac gcctaccagc
     5521 tgatccggag tcaactgagt cccctgagcg gcgagcaagg cagcggaggc acttacaaca
     5581 acctgtttga tgcccatccg cctttccaga tcgatggaaa ttttggttgc acttcgggca
     5641 ttaccgaaat gtttcttcag agtgccgacg gagccgttca cctgctgccc gctttacccg
     5701 aagcctggcc atcggggagt gcgggaggct tgaaagcgag aggaggtttt gagatcgtga
     5761 atatggaatg gaaagactac caactggtaa agctggtggt aaaatcctcc ctcggcggga
     5821 atctgaggtt gcgtttgccc aatgaaatga aaacgggcaa tggaaaagct ctcaacgctg
     5881 cagtgggcaa aaattccaat ccattctatt atctggagga aaccgccccg gccatcctct
     5941 cagaaaaagc aaatctaaaa ctgcttgaac tgaaacccac ccttctggtg gatatcccca
     6001 ctaaaaaagg caaggtttat accttttcag gtcattaata aaaatattac cgcaatttgt
     6061 catccattca accattagga aaataactta ttactcttga accggcttca gcgcttatcg
     6121 gctattctga tccagctcca gtccggccac cgggtgaggg ccggcgacat ggccgaaaga
     6181 ttcggggtca gcctgcgcac cgtttaccgc gatattcaat ccctggaggc taccggcgta
     6241 ccggtgattg gggaggcggg ttccgggtac cggctggtgg atggctaccg tcttcccccg
     6301 gtacatttta cgagggggga gatccatgcc ttgctcaccg ctcagaagct gatcgaaaaa
     6361 caaagcgacc agggcctgga tgccgatttc aaggctgcgc tgttcaagat caaagccatt
     6421 ttgcgtaacc cggaaaagga ccttttgtcc agcctggaag aaaatattga ggtactccac
     6481 aacccttatt tgccggcccc ggaccgcggc aacccctttc tgccaaaatt gatccagtcc
     6541 atttccgaca agaccagggt gtacctggac tataccaacc gtcaggacgt caaaagcagc
     6601 cggcgggaaa ccgagcccat tggccttttt tacctgcttg gaaaatggca cctggtagcc
     6661 tattgccatt tacgcaaaga ttaccgcgat ttccgcctgg accggatccg ggagcttggg
     6721 ctcacttcca aaccctttgc aaaacgacat cccaccttaa aaaaatacct cagccagttg
     6781 acccgggagg aaaaactgaa caccattgaa atccggatga ataaagagga tgtccactat
     6841 ctcggggagc aaaaatatta taacggctat gtctcggaac ggccggacgg ggaccaggtg
     6901 acccttacct tcctgacctc ttccctgacg ggtttcgccc attggtacat gatgatcggt
     6961 acccgggcag aaatcctgaa acccgaaatc cttaaagaac aggtgcaccg catggcaacc
     7021 gaacttttaa aaaatatcaa atagccggtc gctcctgaca tacccttgtc accccccggg
     7081 ccttactttg taaaaaattc aaaaaacatg atctcattta tcgaatcttt caaaaaggaa
     7141 ctggaacaag aggcggtcca gacccggcgt atgctgcaaa tcgtcccggc ggacaagatg
     7201 gactggcgcc cccatcccaa aagcatgacc atcaaggctc ttgccagcca cctggccgaa
     7261 atccccctca tgatcaccat ggccctggac catgacaaat gggattttgc aaaccacccc
     7321 tatgccccgg tggacatgaa caatgccacg gaactggtaa accgttttga acaatgcctc
     7381 gatgaggcca gggcttccct gaaccgggcc accgacgaca tcctgcagaa aaaatgggtg
     7441 atgtgcgcag gagacgccat atacatgacc tgcgagaaat gggaggcggt gcgtcacgcc
     7501 tttggtcaga atgcacacca ccgggcccag ttgggcgttt tcctccgatt gctggatatc
     7561 ccgataccgg ggccttatgg gccgagtgcg gatgagatgt aaaagggcta aaggttaacg
     7621 gcgaagggcg aagggagcaa attattggcg aaaggcgaaa ggttaaaggc gaaaggagca
     7681 taattattgg ggaaaggtca agggcgaagg acgaagggag caaaattcta atcgctaatc
     7741 ctttatttgc tttttgggcc tggtgggctt tgtgggagaa ggaatcccgc agccgtggga
     7801 ggcaaagggc gaaaggtaaa cggcgaaggg cgaagggagc ataattattg gagaaaggga
     7861 ggaagggaga caggagaaag ggaggaaggg aaaacctcta acctctgacc ttatatgtga
     7921 gggaggaatg gagacgggag gaagggagaa agggaaaatc actaacctct gatggctgac
     7981 gctgaaagct gacggctgat acctgatccc tgactactga ctacttcttc tttctcggaa
     8041 tcgacaagct ctcccaaggt ccatttttca attcctttgc gatatggacg atctgcccga
     8101 catggta
//
LOCUS       DAOQXT010000159         8075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_9397_length_8075_cov_6.073317, whole genome shotgun sequence.
ACCESSION   DAOQXT010000159 DAOQXT010000000
VERSION     DAOQXT010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9397_length_8075_cov_6.073317"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="PKM27_18170"
     CDS             complement(<1..1670)
                     /locus_tag="PKM27_18170"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HNR09252.1"
                     /translation="MKPVLTLLIFLLLLACARQEKPAGELPELVGYNTHIRPIINQKC
                     ISCHGGVRKKGGLSFLFPEEMFLPLEDSIPAIIRGDAENSKIMQRILHHDPEERMPQK
                     ADPLSSHEIDLIRKWVDQGAHWETHWAFIPPQHPAIPDVDDKENIGNPIDHFIRARLQ
                     TQGLRANPVAKKEDLIRRLSLDLTGLPPSISDVQAFVSGSLSYDTLVDRLLQSPRFGE
                     RWTAMWLDLARYADTRGYEKDAHRDMWKYRDWLIDAFNADMPFDSFTLRQLAGDLLPG
                     AGPSDLIATAFHRNTMNNDEGGTDNEEFRIAAVIDRVNTTYEVWQGVTMGCVQCHSHP
                     YDPILHEEYYKSFAIFNNTADADRDDQAPTMKVYTHLDQPKADSLKAYLTGVKTRPRP
                     LLPKENLDQMQAYELPIMQELQGAQKRVTRVFERGNFLLKGEVVKAGAPEILQAGKAI
                     EINDRLQFAQWLVSPSNPLTARVMVNRIWAQLFDQGLVTTLEDFGSQGNKPSHPELLD
                     HLAMRFSRDMHWSLKKLIRYLVRSHTYMQSAAVSADQLQKDPGNIWLS"
     gene            1859..2713
                     /locus_tag="PKM27_18175"
     CDS             1859..2713
                     /locus_tag="PKM27_18175"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNR09253.1"
                     /translation="MKSSIILFFVFTACMASLDAQIRTPAASPGAKLEQAVGLSTITV
                     DYSRPSAKGRKVFGDLVPYGQFWRTGANAGSKITFSDDVKISGKEVKKGKYSLFTIPG
                     EMEWTIVLHKNADLNVPGGDGYKMEDEALRFTAKAEKMAKMKETFTIEFDNLKDESVD
                     LFIAWEYTRVAFNIDVNTDTKVVASINAVMSGPSANDYMAAANYYMNSGKDINKAVEW
                     AAKGVSMGANQFWNLRAYSLILAKAGKTSEAIAAAKQSLAKAKEANNNDYVKMNEKSI
                     ADWSAMKK"
     gene            complement(2792..4312)
                     /gene="gpmI"
                     /locus_tag="PKM27_18180"
     CDS             complement(2792..4312)
                     /gene="gpmI"
                     /locus_tag="PKM27_18180"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNR09254.1"
                     /translation="MEVYKLALIILDGWGLGPDYNADAIARANTPFFDQAWQNHPHST
                     LITFGEEVGLPEGQMGNSEVGHLNLGAGRIVYQELARINRAIRTGELKINSELQALLR
                     RCKEKGNTLHLIGLLSDGGVHSHIDHLLALCDIIEPWQLKAVRIHAFTDGRDTDPKMG
                     KTYVSKLLDHLRGKNAALASMVGRYYAMDRDKRWERVKKAYDLLVHGKGELVSDPLTA
                     MENAYKNGVTDEFIEPIAVSGLPNSTIRDGDAVLFFNFRTDRPRQITEALHQKDFPEQ
                     GMHKLDLDYVTMTEYDASYRDVHILFKTDNLNNTLGEVISRLGISQLRMAETEKYPHV
                     TFFFSGGREHPFDLEQRILIPSPKVPTYDLLPQMSAPELTAALLKHWESFSPGFICLN
                     FANTDMVGHTGVFSAGMIAAETVDHCLAQLVEAGLKKGYQFLVLADHGNADCMVNPDG
                     SPNTAHTKNPVPLIYIGDRSLHLAPGKLGDIAPTILQLMGLEIPEEMTGKVLSEEL"
     gene            4434..4820
                     /locus_tag="PKM27_18185"
     CDS             4434..4820
                     /locus_tag="PKM27_18185"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNR09255.1"
                     /translation="MRAISLIFLLAFGIPLGAQSIRTTVDALVRGDREYFNKNFTPEV
                     SLTIEDQEQVVSKPEAIQKIMDFVQSMSIRSGKTIHEGVSKGKDSSMGIGSLLADKSK
                     YRVYVSFKTVEGAKLIHELRFEKDIL"
     gene            complement(4837..5580)
                     /locus_tag="PKM27_18190"
     CDS             complement(4837..5580)
                     /locus_tag="PKM27_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNR09256.1"
                     /translation="MDAWVDQFESPGFIPQDPVSVPHAFQHPAEIEISGLFAAVFSWG
                     LRKTILMKSNRLMDLMDRAPLDFILHHHESDLDKFKDFRHRTFQYEDLLYFIRFLNFH
                     FQHFPSLESAFLPGGKFTDMETSLEYFHHYFFSLEEAPKRTRKHIPHPGSGSTCKRLL
                     MYLRWMVRPADRGVDFGLWRSIRPRDLLIPLDVHVHRVAARLGLIDDKNKNWKTVLEL
                     TGMLKELEPDDPVKYDFALFNLGQLKQPF"
     gene            complement(5619..8036)
                     /gene="infB"
                     /locus_tag="PKM27_18195"
     CDS             complement(5619..8036)
                     /gene="infB"
                     /locus_tag="PKM27_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNR09257.1"
                     /translation="MKAPKKEEEISRAVPETPRLKVVDKIDLEARKKKKPAEAEEPVP
                     AEPVAPQAEEKPRTAAPEEPAVEEAATEEMHRAETPQLKGLKILGKIDTDKFDKAKKK
                     KEEPAATTPADAAKKKLVASSDEELRKKRKRKRKKIETGTPGETDQRGAAAGARPGRT
                     GRDENKEISQKEIDDQIRATMARLGGGARSKRQKIRRDNRSRIREKIEMEEQAQTDAD
                     IQVTEFMTVSELASLLNVKPAQVIMTCMNLGVIVSINQRLDAEIIELVAEEFGHKVIF
                     ISAEESTDSEEEEEDATEDLVPRAPIVTVMGHVDHGKTSLLDYIRSANVVSGEAGGIT
                     QHIGAYEVKVGDKQITFLDTPGHEAFTAMRARGTKMTDVAIIIVAADDNVMPQTKEAI
                     SHAQAANVPIVFAINKIDKPGANPDKIKQELASMNLLVEEWGGKIQSQDISAKTGLNV
                     DKLMEKILLEAEVLDLKANPNRLATGVVIEASLDKGRGYVTKLLVQNGTLHVGDALLA
                     GEHFGRVKAMFNEWGQKTSAADPSSPVLVLGMAGAPQAGEKFKVYEDEAEAKELGLRR
                     AQINRELATRTNKRISLDEIGRRLALGTFKELKVIVKGDVDGSVEALSDSLIKLSIEK
                     IQVSVIHKAVGGITESDVLLASASDAIIIGFQVRPSAAARKLAEKEGVEIKTYSIIYE
                     VINEIKSAMEGMMEPTREEKVIAMVEVREVFKISKVGTVAGCFVTEGKIMKNTPIRLI
                     RDGIVVYPARESNTAKIAALKRFKEDVKEVKSGMECGISIENFSDIKVGDVIEGYEIS
                     EIKAKLN"
BASE COUNT         1799 a         2050 c         2120 g         2106 t
ORIGIN      
        1 tgggacagcc agatattgcc cggatctttt tgaagttgat ctgcgctcac tgcggcggat
       61 tgcatatagg tgtgggaacg gaccaaataa cggatcagtt tttttaagga ccaatgcatg
      121 tcccggctaa aacgcatcgc caggtggtcg agcaattccg ggtgactggg tttattgcct
      181 tgcgaaccaa aatcttcgag ggtggtgacc aaaccctggt caaacagctg ggcccagatc
      241 cggtttacca tcaccctggc ggtaagtgga tttgagggag agaccagcca ttgggcaaat
      301 tgaagccggt cgttgatctc gatggctttg ccggcttgaa ggatttccgg cgccccagct
      361 tttaccactt ctccctttaa aagaaaattg ccccgttcaa aaacccgggt gaccctcttt
      421 tgggcgcctt gcagttcctg catgatgggc agttcgtagg cctgcatctg atccaggttt
      481 tcctttggta acaagggccg gggccttgtt ttgaccccgg tcaagtaagc ttttaaagaa
      541 tcggctttgg gttgatcgag atgcgtgtag accttcatcg tgggcgcctg gtcatcccgg
      601 tccgcatcgg cggtattgtt gaaaatggcg aaggacttgt aatattcttc gtggaggatt
      661 ggatcataag gatggctgtg gcattggaca caacccatgg taaccccctg ccaaacttca
      721 taggtggtgt tcacccgatc gatgaccgcg gcgatccgga attcttcatt atccgtaccg
      781 ccttcatcat tattcatggt gttgcggtgg aaggcagtgg ctatgaggtc ggaggggccg
      841 gctccgggca gcaagtctcc tgcaagctgg cgaagggtga aactgtcgaa cggcatgtcc
      901 gcattaaagg cgtcgatcag ccaatccctg tatttccaca tgtcccggtg cgcatccttt
      961 tcgtaacccc gcgtatctgc gtatcgggcc aggtcgagcc acatggccgt ccatctttct
     1021 ccaaagcggg gagattgaag cagccggtcc accagggtat cgtaggacag ggatccggat
     1081 acaaaagcct gcacgtccga aatcgagggt ggcaatccgg tgaggtcaag gctcagcctg
     1141 cggatcaggt cttccttttt ggcaaccgga tttgcccgaa gtccctgggt ttgcagacgg
     1201 gcacggataa aatggtcgat cgggttgcca atattctcct tgtcgtccac atccgggatg
     1261 gcggggtgtt gagggggaat gaaggcccaa tgcgtttccc aatgcgcccc ctggtcgacc
     1321 cattttctga tcaggtcgat ttcatggctt gaaagcggat ccgctttctg gggcatcctt
     1381 tcttcgggat cgtgatgaag gatcctctgc atgattttgc tgttttccgc atcgccccgg
     1441 ataatggccg ggatactgtc ttcaaggggc agaaacattt cttcagggaa taaaaaactc
     1501 aagccccctt ttttccggac acccccatgg caactgatgc atttttgatt gatgattggc
     1561 cggatatggg tattgtaacc caccaattcg ggcaattccc cggccggttt ttcctgcctt
     1621 gcacaagcca aaagcaataa aaaaatcaac agtgtaagga ctggtttcat ctccgcatga
     1681 aattacaaaa acaggataaa atttgagtca tcccttcact taagtgatcc tctcacttaa
     1741 gtcatcccct cactttaaca tatatttaaa ttatccgtca agcgtaatcc acaggctgtt
     1801 aacggttatt tttagttaat tttgcgcttt aattttctaa acaataaact cattcaaaat
     1861 gaaaagttcg atcatcctgt tttttgtgtt taccgcctgc atggccagcc tggacgccca
     1921 gatccgtacg ccggctgcca gccccggtgc gaaactcgag caagcggtgg gcttgtccac
     1981 catcacggtt gactatagcc gtccaagcgc caaaggccgt aaagtattcg gcgacctcgt
     2041 tccttatggc caattttgga ggaccggcgc gaatgcaggt tcaaaaatca cttttagcga
     2101 tgatgtgaaa atcagcggta aagaagttaa aaaaggcaag tattccttgt ttaccatccc
     2161 cggcgaaatg gaatggacca tcgtccttca caaaaacgcc gacctgaacg taccgggtgg
     2221 agacggctat aaaatggaag acgaggcact ccgctttaca gctaaagccg agaaaatggc
     2281 caaaatgaaa gaaaccttta ccatcgagtt tgacaacctg aaagatgaaa gcgttgacct
     2341 gttcattgcc tgggaatata cccgtgtggc cttcaacatc gatgtcaata cggataccaa
     2401 agtcgtggcc agcatcaatg cggtcatgag cgggccttct gccaatgatt acatggccgc
     2461 cgccaattat tatatgaaca gcggcaaaga catcaataaa gccgtagaat gggctgccaa
     2521 aggggtaagc atgggtgcca accaattctg gaacctgagg gcatacagcc ttatcctggc
     2581 caaggccgga aagacttctg aagccatcgc cgctgccaaa caatccctgg ccaaagccaa
     2641 ggaagccaat aataacgact atgtcaaaat gaatgaaaaa tccattgcgg attggagcgc
     2701 tatgaaaaaa taagccttca gacgaactta aagaaaaggc cgttgtcccc gacagcggcc
     2761 ttttttattg ctttaaactt taacatggaa ctcacagttc ctcactcaga acctttccgg
     2821 tcatttcttc cggaatttcc aatcccatca gttgcaggat ggtcggggcg atgtctccca
     2881 gcttgccggg cgccaggtgc aaggaacggt cgccgatata gatcaggggc actggatttt
     2941 tggtatgggc ggtattcggg gaaccatcgg gattgaccat gcagtcagca ttgccgtgat
     3001 cggccagtac cagaaactgg tatccttttt tgaggccggc ctccaccagt tgggcaaggc
     3061 agtggtccac cgtttctgcg gcgatcatgc ccgcgctgaa aacaccggta tggcccacca
     3121 tatcggtatt tgcaaaattg aggcagataa aaccgggact gaacgattcc caatgtttga
     3181 gcagggcggc cgtaagctcc ggagccgaca tctggggcag caggtcgtaa gtggggacct
     3241 tgggagaagg aatgaggata cgctgctcca gatcaaacgg atgttcccgg ccaccggaaa
     3301 agaaaaaagt gacatgggga tatttttcag tctccgccat ccggagttgg gatatgccca
     3361 gccgggatat tacttcccca agcgtgttgt tgagattgtc cgtcttaaaa aggatatgta
     3421 cgtccctgta gctggcatcg tattccgtca tcgtcacata atcgaggtcc agtttgtgca
     3481 tgccctgttc aggaaaatcc ttttgatgca gggcttccgt gatttgcctg gggcggtcgg
     3541 tccggaaatt gaaaaataaa acagcgtccc cgtcccggat ggtggagttg ggtaggccgc
     3601 tgaccgcgat gggctcaatg aattcatccg tgacgccgtt tttataggcg ttttccatgg
     3661 cggtcaaggg atcggaaacc aattccccct tgccatgaac caggagatca taggcttttt
     3721 tgacccgttc ccaccttttg tcacggtcca tagcataata ccttcccacc atggaggcca
     3781 gcgccgcatt ttttccacgg aggtggtcca ggagctttga gacataggtt ttgcccattt
     3841 tagggtcggt atcgcgtcca tcggtgaagg catggatccg gacggctttg agctgccagg
     3901 gctcaatgat atcgcaaagg gcaagcaaat ggtcgatatg ggaatgtacg cctccatcgg
     3961 aaagcaggcc aatcaggtga agggtattgc ctttttcctt acagcggcgg agcagggcct
     4021 ggagttcgga atttattttg agttctccgg tacggatggc gcggttgatc cgggccaatt
     4081 cctggtatac gattcgccct gcaccgaggt tgagatgtcc tacttctgaa tttcccatct
     4141 ggccctctgg gaggccgact tcttcaccaa aagtgatcag ggtgctgtgg ggatgatttt
     4201 gccaggcctg gtcaaaaaac ggggtattgg cccgggcgat ggcatccgca ttataatccg
     4261 ggcccaggcc ccagccatcg agaatgatca gggccagttt atacacttcc attctgaaaa
     4321 tttttgcaaa attagggttt gccgttcatt ggtaacttta tattttaact attaattagc
     4381 taaatatttg aacaaagaag cgtctgttaa aggttttaaa taaaatgatg aaaatgagag
     4441 caatctcttt aatattcctg cttgccttcg gtattcctct aggggcccaa tccatccgga
     4501 cgacggtgga tgccctcgtc agaggggaca gggaatattt caataaaaac tttacgccgg
     4561 aagtttcgct caccattgaa gaccaggagc aagtggtgtc caaaccggaa gccattcaaa
     4621 aaataatgga ttttgtacaa tccatgtcca tccggtccgg gaagactatt catgaagggg
     4681 tcagcaaagg gaaggattcc agcatgggca tcggaagcct gttggctgat aaatccaaat
     4741 accgggtata cgtttcattc aaaaccgtgg aaggagcaaa attgatccat gagctccgtt
     4801 ttgaaaaaga catcctttaa aaaatcatct ccccggtcag aaaggctgtt tcaactggcc
     4861 caggttaaaa agggcaaaat catacttgac cggatcgtcg ggttcgagtt ccttgagcat
     4921 cccggttagt tccagaactg ttttccagtt tttgtttttg tcatcgatca gtcccaaccg
     4981 ggcggcaacc cggtggacat ggacatccag ggggatgagc aggtcccggg ggcgaatgga
     5041 ccgccagagg ccgaaatcca caccccggtc ggctggcctg accatccagc gcagatacat
     5101 caatagcctt ttgcaggtcg atcccgagcc ggggtggggg atgtgcttcc gggtccgctt
     5161 cggggcctcc tccaggctga aaaaataatg atggaagtat tcaaggctgg tttccatatc
     5221 ggtaaatttt cctcccggca ggaaggcgga ttccagggaa ggaaagtgct ggaagtgaaa
     5281 gttcaggaac cggatgaaat aaagcaaatc ctcatattgg aaggtacggt gcctgaaatc
     5341 tttaaattta tcaagatccg attcgtggtg gtgcagtata aaatccagag gggcccggtc
     5401 cataaggtcc atgagcctgt tgctcttcat gaggatggtt tttcgcaggc cccatgaaaa
     5461 aacggcggca aacaggccgc tgatctcgat ctcggcggga tgttgaaaag catggggtac
     5521 ggataccgga tcctgcggga tgaatccggg cgattcaaat tgatccaccc aggcatccat
     5581 cctggatttt aaagttccgg ggtccggaga ttgcccgatc agtttaattt ggctttgatt
     5641 tcactgattt catacccttc aatgacatcc cctaccttga tatcgctgaa attttcaatg
     5701 gatatcccgc attccatccc gcttttaacc tctttgacat cttctttgaa ccgtttgagg
     5761 gccgcgatct tggcggtatt gctttcacgg gctggataaa ccacgatgcc gtcccggatg
     5821 aggcggatcg gggtattttt catgatcttc ccttccgtaa caaaacaacc ggctacggtg
     5881 cccactttgg aaattttgaa gacttcccgg acttcaacca tcgcgatcac tttttcctcg
     5941 cgggtgggtt ccatcatgcc ctccatggct gatttgattt cattgatgac ttcgtagatg
     6001 atggagtaag tcttgatctc gaccccttcc ttttcagcca gtttccgggc ggccgccgaa
     6061 ggcctcacct ggaaaccgat gatgatggca tccgaagcgg aagccaggag tacatcggat
     6121 tccgtgattc cgcccactgc cttgtggatc accgatacct gtattttttc tatggaaagt
     6181 ttgatcaggg agtcagacaa agcttccacg gaaccatcca cgtcgccttt gacgatgact
     6241 ttgagttcct tgaaagtacc cagggccaga cgtcttccga tttcatccag ggagatccgt
     6301 ttattggtac gggttgccag ttcccggttg atctgtgccc ggcgcagacc cagttctttg
     6361 gcttcggctt catcttcgta gactttaaat ttttctcctg cctggggagc gccggccatt
     6421 cctagtacga gcaccgggct ggaaggatcc gcggcggacg ttttctggcc ccattcatta
     6481 aacatggcct tgacccgtcc gaaatgttct ccggccagta aggcatcgcc cacatgcagc
     6541 gtcccgttct gaacgagtaa tttggtcaca tagcccctgc ctttgtccag cgaagcctcg
     6601 atgacgaccc ccgtcgcgag ccggttggga ttggctttca gatcgagcac ttcggcttcc
     6661 agcaggatct tttccatgag tttgtctacg ttcaggccgg tttttgcaga gatgtcctgg
     6721 gactggatct taccgcccca ttcttccacc aggaggttca tggatgcgag ttcctgtttt
     6781 attttatcgg ggttggcccc gggtttatct attttgttga tggcgaagac aatgggcaca
     6841 ttagcagcct gggcgtgcga aatggcttcc ttggtctggg gcatgacgtt gtcgtccgca
     6901 gccactataa taatggctac gtcggtcatc ttggttcccc tggcccgcat agcggtaaac
     6961 gcctcgtggc cgggggtatc caggaaggta atctgtttgt ctccgacttt tacctcatag
     7021 gcaccgatgt gctgggtgat gccgccggct tccccggaaa ccacgttagc actccggata
     7081 tagtcgagga gggaagtttt cccgtgatcg acgtggccca tgaccgtgac tatgggcgcc
     7141 cggggaacca gatcctccgt tgcatcttct tcctcctctt ccgaatcggt ggattcttcc
     7201 gcagaaataa agatgacctt gtgtccaaat tcttcggcca cgagttcaat gatctcggca
     7261 tccaggcgct gattgatgga tacgatcact cccaggttca tgcaggtcat gatcacctgg
     7321 gcgggtttta cattaagcag ggaggcaagt tctgaaacgg tcatgaactc ggtgacctgg
     7381 atatcggcat ccgtttgggc ctgctcttcc atttcgattt tttccctgat ccgcgaacga
     7441 ttgtccctcc tgattttctg gcgtttgctt cgggcgcctc cgcccaggcg ggccatggtc
     7501 gcccggatct ggtcgtcaat ctctttttgg gagatttctt tattctcatc ccttccggtt
     7561 ctgcctggcc gggcccctgc ggccgctcct ctttgatcgg tttcgcccgg ggtacccgtt
     7621 tcgatctttt tacgtttgcg tttccttttc tttcttaatt cctcatcaga ggaggccacc
     7681 agttttttct tggcggcatc ggcgggagtg gttgcagcgg gttcttcctt ctttttctta
     7741 gccttatcaa atttgtcggt atcgatcttg cccaggatct taagtccttt caattgcggg
     7801 gtttcggccc ggtgcatttc ttccgtggcg gcttcttcca cggcgggttc ttccggtgcg
     7861 gcggtcctgg gtttttcttc agcctggggt gctaccggct ctgcagggac cggttcttcc
     7921 gcttccgccg gctttttctt tttacgcgct tccaggtcaa ttttatccac caccttcaac
     7981 ctgggtgttt cggggaccgc gcggctgatt tcctcttctt tcttcggggc cttaatctcg
     8041 gcaggggctt cctggacggg tttggccggc ctggg
//
LOCUS       DAOQXT010000160         7996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_9497_length_7996_cov_7.292029, whole genome shotgun sequence.
ACCESSION   DAOQXT010000160 DAOQXT010000000
VERSION     DAOQXT010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9497_length_7996_cov_7.292029"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..3539
                     /locus_tag="PKM27_18200"
     CDS             486..3539
                     /locus_tag="PKM27_18200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR09258.1"
                     /translation="MNNKPLLCIAVCMLFCLQLFAQDRQVSGTVKDGTGSGLPGVTVL
                     QKNSTLGTITDVDGNFSMSVPNNQTVLVFSYVGFSTMEVAVGEQSQLSIVLSEDVSTL
                     SEIVVTGYGTQRKSQLTGAISSISAKQISELPITNLAQALTGRATGVNVTQSGSKPGS
                     VPKILIRGRRSFNAGNDPLYVVDGIPLSAGYEDINPNDIQSLEVLKDATATAIYGARG
                     ANGVVLVTTKRGSSKKGKTTITLDTYAGRSDALDKIELFSGPEFAEYVREAYRATGGY
                     KDAAGNPVPTGVADAAADAKVAVLGGDPEVAAGILANRNTNYQDLILKPGLMQNHTLG
                     VQGGNERTAFYLSAGFFQDKGITKGLDYTRYSLRANIDHTINNFLNVGLSSYMMFSDR
                     NGENLNPYSFTLQQNPLGRPYDDNGNLIFSPTNDALLTNPLFEIIPGAQVDNTKRYRI
                     FNSIYAEAKITNGLKYRVNFGPDFTLSRWGRFIGSLTNARKGGDAQALNENRFGFDWT
                     LENIVTYEKAVGRHQFNFTGLQSIQKDRFELYRTEVTGVPAETQKFFSLGTAGAVQAI
                     RSDLVEWTINSYMGRLNYAFDDKYLLTLTLRRDGSSRFGENTKYGNFPGVAFGWNIAH
                     EAFMKSVNWVDALKLRAGWGKVGNQAVSPYQTQGLLSRTTYAWGTTAAFGYRPSTIGN
                     PDLRWESSATANLGLDFSIFKGRLQGTIEVYKTDTESLLLSDFLPGSTGFNAVTRNVG
                     HTRNKGIEFNITSTNINTNSFKWETDLAFSKNTEAIVELYNGKVDDLGNRWFIGKPLN
                     TYFDYKKIGIWQTNEADLAKSFGSEVGQIKIQDTNGDGRINAADRVILGSDIPDFQAG
                     LTNRFSYKGFDFNFFLYASIGQKIVSGFHQNNNALAGRYQQIKVDYWTPNNPTNEFPR
                     PKSTQEFPVLNTSLIYFDGSFIKLRNVNFGYTFPASWSKKLRAESVRVFSSIQQPFIW
                     SEFRSKYNGVDPEATITSSANGITAVNNGITPATRVVTFGFNFKF"
     gene            3562..5244
                     /locus_tag="PKM27_18205"
     CDS             3562..5244
                     /locus_tag="PKM27_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR09259.1"
                     /translation="MKNSLKNILKTSLMAGLLLTSFSCGKDLLDEEVISNIGNDYMNT
                     PKGYEDAVRAAYSTLRYFYGTQQGLTLTEYGTDIYATGADGGYKGFHFYDAQLQPTVD
                     YLINTWDELYRGINTCNAIIERAPNVAGLSDAVKKLRVAEAKFLRAHYYYILHQQYGG
                     VHLKLSETLSPSKSTSRASDAEMYAAIIKDCNEAIADLENKGHSSNYGRATKATAETL
                     LAKVLLAKAYGPSKAGDDFQKAADLCTNIINNYGFKLLDDFASVHDENNQVNAEVVFA
                     VQYTTDQLANTTNNTGAANGGNNLHLFFGMQYDVQPGMKRDVFYGRPFKRLRPTAYCL
                     NTVFGERTNDSRYNKTFRTVWLSNNPGTNLNSSFDNSKAKLSFAAGDTCIVIPGYEMS
                     KEERATKKYQVLVPSAYNEALFPTLTKFFDTKRADLTDERGSRDYIVWRLADIYLMQA
                     EALLQLGKISEATAAINVVRVRAGYPGKKDLMKITEAQMTMDFLIEERARELAGEQMR
                     WMDLKRWGLLVDRVKKYNPQASAISDKHNVRPIPQTQIDRSEAGSFAQNPGY"
     gene            5571..6551
                     /locus_tag="PKM27_18210"
     CDS             5571..6551
                     /locus_tag="PKM27_18210"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNR09260.1"
                     /translation="MERRDFIQQGFAFSMIPGSAASRFKSEAAPAEQVVIERPVSGQP
                     HKGKVLAVIQPHCDDISLFAAGTVAKLIKEGYEGYMIRTTNDDHAGRGETVGDVVKNN
                     LVSNAMVAKALGLKKVFDLGYRNHMMDNYSMQDIRGRLIFLFRVLQVDTLICYDPWGS
                     YEENPDHYITARAVEAARWMAGGGRDYPEHFEAGLKPKGVAERYYFARGPQLFNRVVD
                     ITEVLEKKIDANLANFSQGPAGEAGAVLRRKLADQGKYLPLLGQNDNEANRNYIKHFV
                     LDMDSLYLRGVPSDRQIGEAYQLGWAERFHYIGPEPSVLPEYLEKNVRNK"
     gene            complement(6694..7806)
                     /locus_tag="PKM27_18215"
     CDS             complement(6694..7806)
                     /locus_tag="PKM27_18215"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNR09261.1"
                     /translation="MIKTMISFCLALVLSGSIHSEEVKTLEIGASAPDFSLKGVDGKM
                     YTLTSFSKADILVILFSCNHCPTAQAYEDRMIKFVNDYKSKGVTMVVISPNSDAAVRF
                     DELGYTDLSDSYEEMIMRAKDKNYNFPYLYDGATQEVSKKYGPVATPHAFVFDKSRKL
                     RYQGRIDDNEHIGREKVHDLKNAVDALLKGAPVAVQTTKVFGCSVKWAEKSASKVSEV
                     QGWAKEPVALEKASLEQVKQLVKNEGTGKYRLINVWATWCGPCVREFTGLVESHHMYR
                     RRDFEFVSISMDAPKNYDKALALLKEKLASNKNYIYDGDDKYAFIEAVDPNWQGALPY
                     TLLISPEGKVVYNQMGLLDIVKLRKHIADHIGRYYD"
BASE COUNT         2146 a         1817 c         2004 g         2029 t
ORIGIN      
        1 tgaaaaagca ggaggctaaa gttgaaaacg gtcaaccaaa ttcaggcatg gacagacctc
       61 taacctctaa cctctaacct ctaacctcct actctgattc gtattaaata ttctcaggaa
      121 aggtttaaag cgcgtcattg gctcggggca gtcgtggatt tacagaggtt cttgcagtat
      181 atgaaagcca aaattctact ggccgggaat accggtggat tgtctggtgg ataccggatt
      241 caaatcatga aatatcggaa acggtaccgg tatcaggtgc ttggtgatct acctgagcag
      301 cccatattta aatattcgtt gattatcaat aaattgagta aaaaaaatta aaaattttcg
      361 ggcgacaaaa cgttgagttt aaaaccggat gttcctcaat ccaaaacatc caggagttat
      421 aaattatgaa taagaaattt tagcttcatc cgggattact caaatttctt aaaactattt
      481 tttgtatgaa taacaaaccc ctattatgta tagctgtgtg catgctattt tgccttcagc
      541 ttttcgccca ggacagacag gtttcgggta cggtcaagga cgggactggc tccggcttac
      601 ctggagtcac cgtgcttcag aaaaattcaa ccctcggaac catcacagat gttgatggga
      661 atttttcgat gagcgtaccc aataatcaga ctgttttggt attcagttat gtgggattta
      721 gcactatgga agtggctgtc ggagaacaaa gccagttaag catagtgctt tccgaagatg
      781 tcagtaccct ttcggagatc gtggtcacgg gatatggtac gcagcgaaag agtcaattga
      841 caggcgctat ttcttccata tcggcaaaac aaatttccga acttccgatc acaaacctgg
      901 cacaggcgct tacgggtcgc gctaccggag tcaacgttac ccaatcgggt tctaaaccgg
      961 gctctgtccc aaagatcctg atccggggcc gtcgttcctt taatgccggg aatgatccgt
     1021 tgtacgtagt cgatggtatt ccgctttctg ccggatacga ggatatcaac cccaatgaca
     1081 ttcaatcatt ggaggtcttg aaggacgcca cggccacggc aatctacgga gcaagggggg
     1141 ccaacggagt ggtgctggtc actacaaaaa gaggttcttc caaaaagggt aaaaccacga
     1201 ttaccctgga tacctacgcc gggcggtctg atgctttgga taaaatagag cttttttcag
     1261 gcccggaatt tgcagaatat gtaagagagg cttaccgggc aacgggcgga tacaaagatg
     1321 cggctgggaa tccggtccct acgggtgtgg ccgatgccgc agcggatgcc aaggtggccg
     1381 ttttaggcgg tgacccagaa gtggccgcag gcattttagc caatcgcaac accaattacc
     1441 aggacctgat attaaaaccc ggattaatgc agaatcacac ccttggagtg caggggggta
     1501 atgagaggac tgccttttat ctctcggcgg gattttttca ggataagggg ataaccaaag
     1561 gcctggatta cacccggtat tcattaaggg caaacattga ccatacgatc aacaattttt
     1621 tgaatgtggg tctttcttct tacatgatgt tttccgacag aaacggagaa aaccttaacc
     1681 cttattcatt caccctgcaa caaaatccgt tgggacgtcc ttacgatgat aacggtaatc
     1741 ttattttttc tccaacgaac gatgcgctgc ttacgaatcc gctgtttgaa atcattcctg
     1801 gagcccaggt ggacaatacc aagagataca ggatcttcaa ttcaatttat gcagaagcaa
     1861 aaattaccaa tgggcttaaa taccgggtaa attttggacc ggactttacc ctgtcgcgtt
     1921 ggggtagatt cattggtagt ttaaccaatg cgcgaaaagg cggagatgcc caggcattaa
     1981 atgaaaacag atttggattt gactggacat tggaaaatat tgtgacctat gaaaaagcgg
     2041 ttggaagaca tcaattcaat tttaccggac ttcaatccat tcaaaaggac cgttttgaat
     2101 tgtaccgtac ggaagtgacg ggcgtccctg ccgaaacaca gaaatttttt agcctgggta
     2161 cggccggcgc cgttcaggcc atccggtcgg acctggtaga atggacgatc aattcataca
     2221 tgggccgttt aaactatgct tttgatgata aatatttgtt gacgcttacg ctccgccggg
     2281 atggatcgag ccggtttgga gaaaatacca aatacggtaa ctttcccgga gtggcttttg
     2341 gatggaatat tgcccatgag gcatttatga aatccgtcaa ctgggttgat gcattgaaac
     2401 tccgggccgg ctggggtaaa gtgggcaacc aggcggtctc tccctatcaa acccaaggct
     2461 tgttaagccg gaccacctat gcctggggga ccacggcggc ttttggctac aggcccagta
     2521 cgatcggaaa tcccgatttg agatgggaat cttcggctac tgcaaattta ggactggact
     2581 tttccatttt taagggaaga ttacaaggga caatcgaggt gtataaaacg gataccgaat
     2641 ccctgctgtt gtctgacttt ttaccgggtt ctaccggttt taatgcggtc acccgcaatg
     2701 tggggcatac ccggaataag ggcatcgaat ttaacattac ttcaacgaac ataaatacca
     2761 acagttttaa atgggaaacg gatttggcct tttccaaaaa cacggaagca attgtagaat
     2821 tgtataatgg aaaggtggat gatttgggaa accgttggtt tatcggaaag cccctgaata
     2881 cctattttga ttacaaaaaa atcgggatct ggcaaaccaa tgaagcggat ctcgctaaat
     2941 cgttcggttc cgaagtgggg cagataaaaa ttcaggatac gaatggggac ggcagaatca
     3001 atgcagcgga ccgggtcatc ttaggcagtg atatccctga tttccaggct ggtttgacca
     3061 accgtttttc gtacaaggga ttcgacttca attttttcct ttacgccagc attggtcaaa
     3121 agatcgtatc cggtttccac caaaacaata acgcactggc cgggcgttac caacagataa
     3181 aagtggatta ctggactccc aacaacccaa ccaatgagtt tccccggccc aaaagcaccc
     3241 aggagttccc cgttttaaat acgtcgttga tttattttga cggaagcttt attaaattgc
     3301 ggaatgtcaa ttttggatat acctttccgg cttcgtggtc taaaaaattg agggcagagt
     3361 ccgttcgggt attctccagc atccaacaac cttttatctg gtctgagttc agaagcaaat
     3421 acaatggggt ggatccggaa gctacgatta ccagctcagc gaacggtatt acggccgtta
     3481 ataatggaat caccccggca acccgggtgg tgacctttgg atttaatttt aagttttagc
     3541 gtttaaaatt cattttttaa catgaaaaat tcattaaaaa atatattaaa aacaagcctg
     3601 atggcaggcc tgttgctgac ctccttctct tgtggaaaag atcttttgga tgaagaggtg
     3661 atctccaata ttgggaatga ttatatgaat actccgaaag gttatgagga tgccgtccga
     3721 gcggcctatt cgaccctcag gtacttttac ggtacccagc aggggctgac cctgacagag
     3781 tatggcacgg acatctatgc cacgggggcc gacggagggt ataaaggatt tcacttttac
     3841 gatgcccaat tgcagcctac cgtggattat ctgatcaata cctgggatga gttgtaccgg
     3901 ggcatcaata cttgcaatgc catcattgaa agggccccga atgtcgcggg actttcagac
     3961 gcggttaaaa aattgagggt tgccgaagct aaatttttac gcgcacatta ttattatatt
     4021 ttgcatcagc agtatggggg ggtgcatctt aaactgagcg aaaccctgtc gccctccaaa
     4081 tcaacgagcc gggcatcgga cgcggagatg tatgccgcca tcattaaaga ttgcaatgaa
     4141 gccattgccg atcttgaaaa caagggtcat tcatcaaatt acggaagggc aaccaaggcc
     4201 acggcggaaa ccttgctggc caaggtgctt ctcgccaaag cctatggacc ttccaaggcc
     4261 ggtgatgatt ttcaaaaggc ggcggactta tgcacgaaca tcataaacaa ttacggattt
     4321 aaattgctcg atgattttgc ttccgtgcat gatgagaaca accaggtcaa tgcggaagtg
     4381 gttttcgccg tgcaatacac cacggatcaa ctggccaata cgacaaacaa tacgggcgcc
     4441 gcaaatggag ggaacaacct gcatttattt tttgggatgc aatacgatgt tcagccggga
     4501 atgaaaagag atgtatttta tggccgcccc tttaaacggc tgcgtcccac cgcctactgc
     4561 ctgaatacgg tattcggaga acgcaccaac gattccaggt ataataaaac cttccggacg
     4621 gtttggctgt ccaataaccc ggggaccaat ctcaattcct cctttgataa ttctaaagct
     4681 aaactcagtt ttgctgcggg ggatacatgt attgttatcc cagggtatga aatgagcaag
     4741 gaagaaagag cgacgaaaaa ataccaggta ttggtgccca gtgcatacaa tgaagccctt
     4801 tttccgacct taaccaagtt ttttgacacc aaacgggccg accttaccga tgaaaggggg
     4861 tcgagggatt atatcgtgtg gagattggcc gacatctatt taatgcaggc ggaagcttta
     4921 cttcaattgg gaaagatcag tgaagccacg gctgccatca atgtggtccg ggtccgtgcc
     4981 gggtatccgg gtaaaaagga tttgatgaaa attactgaag cccagatgac catggatttt
     5041 ttgattgaag agcgtgcacg tgaactggct ggagagcaaa tgcgttggat ggatctgaag
     5101 cgctggggtt tgctggtgga cagagtaaaa aagtataatc cccaggcttc tgctatttcc
     5161 gataaacaca atgtaagacc gattccacaa acccaaattg atcgctccga ggcaggatct
     5221 tttgcgcaaa acccgggtta ttgataccgg tcctgtttaa attatagttt agagaatgga
     5281 taaggccccg gtcaggggcc ttatctttta gcatgggttt gattcccttt aaaggaatcc
     5341 atggtcagga ttggggaatc cctcgattaa actcaggata aatggccatc acgaaccaaa
     5401 cgttccccgt tatacagaaa atttgattct ggatatacgg accaacagaa catggccggg
     5461 caggaaactt accggtcacc cccgccctcc tggatttcag gggcaaaaca acgtgggctg
     5521 ccgggtcttc tttttaatga ttaacttaac gcggttcaat aattacttga atggaaagaa
     5581 gagactttat acagcaggga tttgcctttt ccatgatccc tggatcggcc gccagccgtt
     5641 ttaaaagcga ggccgcaccg gccgaacagg tcgtgatcga gaggcccgtt tccggtcaac
     5701 cccataaggg aaaagtcctg gcggtgatcc agccccattg cgacgatatt tccctattcg
     5761 ccgcgggcac ggtggccaag ctgatcaaag aagggtatga gggctatatg atccggacca
     5821 ccaacgatga tcatgccgga agaggggaga ccgtggggga tgtcgttaaa aacaacctgg
     5881 tttccaatgc gatggtggcc aaggccctgg gtttgaaaaa agtatttgac ctcggctacc
     5941 gcaatcatat gatggacaat tacagcatgc aggacatccg gggcaggctg atctttttat
     6001 tccgggtctt gcaggtggat accctgatct gttacgatcc ctggggttcc tacgaggaaa
     6061 acccggatca ctacatcacc gcccgggcgg tggaagccgc gcgctggatg gccggtggag
     6121 gccgtgacta cccggaacat tttgaagccg ggctcaaacc caaaggggtc gccgaacgct
     6181 attattttgc ccgcggccct caattgttca accgggtggt ggacatcacc gaggtcctgg
     6241 agaaaaagat cgatgcaaac ctggccaatt tttcacaggg cccggcgggg gaagccggag
     6301 ccgtcctgcg gcgaaaactg gcagaccaag ggaaatatct tcccctcctc ggtcaaaacg
     6361 ataacgaggc caaccgcaat tatatcaaac attttgtact ggacatggat tccctctatc
     6421 tccggggtgt gccctccgac cgacagattg gggaggctta ccagctcggc tgggccgaac
     6481 gcttccatta tatcgggcct gaaccttctg ttttgcctga gtatttggag aaaaatgtga
     6541 ggaataagta gtcagcagtc agaaatcagc ggtcagtagt cagcggtcag tagtcagcag
     6601 tcagcagtca gcggtcagta gtcagtagtc agtagtcagt tgcccgaaag ctgaaatctg
     6661 aaatctgaaa gctgatcgct gatcgctgat cgctcaatca taatatctcc cgatatgatc
     6721 cgcgatatgt tttctcaatt ttacgatatc caacagcccc atttgattgt agaccacctt
     6781 gccttccggg gaaatcaata aggtataggg aagggcgccc tgccagttgg gatccacggc
     6841 ttcgatgaaa gcatatttat catcgccgtc gtagatgtag tttttattgg atgccagttt
     6901 ttccttgagc agggcgaggg ccttgtcgta gttttttggg gcgtccatgg agatggacac
     6961 aaattcaaaa tcacggcggc ggtacatgtg gtgggattcc accaggccgg tgaattcgcg
     7021 aacgcaggga ccgcaccagg tggcccacac gttgatcagg cggtatttgc ccgtgccttc
     7081 attttttacc agttgtttta cctgttcgag gctggctttt tccagcgcca cgggttcttt
     7141 ggcccagccc tggacctcgg aaaccttgct ggcggacttc tcagcccatt taacggagca
     7201 gccaaatacc ttggtggtct gaacggccac cggggcccct ttaagcaggg catccaccgc
     7261 atttttcaag tcatggactt tttccctgcc gatgtgttcg ttgtcatcga tcctgccctg
     7321 gtatctcagt tttcgggatt tgtcaaaaac aaaggcatga ggagtcgcca cgggcccgta
     7381 ttttttggaa acctcctggg tggctccatc atagagataa gggaagttgt agtttttatc
     7441 ctttgccctc atgatcatct cctcataact gtcgctcaga tcggtatagc cgagctcatc
     7501 gaaccggacc gcagcatccg aattcggact gatgaccacc atggtaaccc ctttggattt
     7561 ataatcattg acgaatttga tcatccggtc ctcataggcc tgggccgtcg gacagtgatt
     7621 gcatgaaaac aaaatgacga ggatatccgc tttggaaaaa ctggtcaggg tatacatctt
     7681 cccatccact cctttcaggc tgaagtcggg cgcggaggcg ccgatttcca gggtttttac
     7741 ttcttccgaa tggatagagc cgctcagcac caaggccaaa cagaaggaga tcatcgtttt
     7801 aatcatatct tataatcctg gtttaaaaat aaatttaaag gaatacccat gcacctgcaa
     7861 gggattgaag gaattcttca aaggatggat aagattgagc aggggaatgg aggttagagg
     7921 ttagaggtta gaggttagag acctgtccat gcctgaattt ggttgaccgt tttcaacttt
     7981 agcctcctgc tttttc
//
LOCUS       DAOQXT010000161         7861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_9681_length_7861_cov_7.346785, whole genome shotgun sequence.
ACCESSION   DAOQXT010000161 DAOQXT010000000
VERSION     DAOQXT010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9681_length_7861_cov_7.346785"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PKM27_18220"
     CDS             complement(<1..1171)
                     /locus_tag="PKM27_18220"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNR09262.1"
                     /translation="MTDKSFSQESLSSSGPGSYFRRLGEDGWAVWIAGVLIGGVLGFA
                     LLNPDYKFVSPAYAWKDGGELGSRVFSASNLTTLGILGILYLALAWFLVRKFAGFLVL
                     FLLACISLVLAGNKTVNAYGVEYVVFGLLTGLLLSNLNLAPAWLREAARSEFFIKTGL
                     VILGSSVLFTDIVKAGLPGILQAILVVLVVWFFALWLCRKMKVDDEFGVILASAVSIC
                     GVSAAIVASGAIQGDKKKLSYITTLVLLVAIPMLVLQPWAIRYFNIPELVGGAWLGGT
                     LDTTASVTAAAQLVGPLATKAGVIVKFSQNVLMGVAAFLIAGWWAYSKNKVQEARGLS
                     LKTIWQRFPVFVLGFIAVSLVFSFLLSGETTKQVSGFLNALRTLWFALAFVSIGLE"
     assembly_gap    1303..1402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1508..4063)
                     /locus_tag="PKM27_18225"
     CDS             complement(1508..4063)
                     /locus_tag="PKM27_18225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR09263.1"
                     /translation="MKSFIIITIFFLGLGSRSFAQTTLEGVIKSPDGEPLEGATVLVK
                     GTNRYGIADADGKFILSGLPQTPYTLETSFLGFRKSEIQITNIPSTPVEIILAEDNLL
                     AEIVITARRRQETAQEVPVAISVVSGVFAEQAGAFNVNRIKEYIPSVQLYTSNPRNTG
                     INIRGVGSPFGLTNDGLDPGVGFYVDGVYYARPAAATFDFIDIEQIEVLRGPQGTLFG
                     KNTTAGAFNITSRKASFIPGADFELSYGNYGYIQAKTSVTGPLGNRFAGRLSFSGTQR
                     DGVIENIRTNKFINDQNNLGVRGQLLFKSSENTSLTLAVDASRQRPDGYAQVAAGVAP
                     TKRAAYRQFNAIIADLNYSLPSLNAFDRKIDHDTPWNSGNELGGVSLNLDSKIGPGTL
                     TATTAWRYWNWDPSNDRDFTGLQALEKSQNPAKHRNWSQEIRYAGEVNSRLSGVLGLY
                     FIDQEVKINGTEQSGRDQWRFSQSSTSPLWKTAGLFEGYGISTKASIQSISAAAFANV
                     DWEILEGLHLQPGLRFNIDKKDVSYKRTAFGGLQTNDPALLALKNGIYTSQSYEADAD
                     ESNLTYLVTLAYRASKRLNAFASYSTSFKPVGVNVAGLPTISGKPATELAVIKPEYLK
                     HIEIGLKTSPVENSTLNLTFHHTDIDDYQTNVQSPELGVNRGYIANAEKVRVFGVELD
                     GNIKVNNNFSFYTALAYTEGKYVSFKNAPLPLEETGQTIDGVQVAFKDVSGGDLPGLS
                     RWAASLGGEYTRPAKFLSKAGHFFIALDSYYRSGFSSSPSPSAYLNIDGYTLLNARAG
                     IRVLTGTSIFIWSRNLLNKNYHEQLLPAGGNAGLYASVLGDPLTFGLTLKHAF"
     gene            complement(4406..4834)
                     /locus_tag="PKM27_18230"
     CDS             complement(4406..4834)
                     /locus_tag="PKM27_18230"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNR09264.1"
                     /translation="MMSMKCKYALKALIQLGMGYDKGYIMASTISEAENIPKKFLEQI
                     LLELKHAGFVGSKQGYGGGYYLKRKPAEISVADVYRLFDGAISLVPCVSLNFYEKCDD
                     CKSEKVCLLKPEFLKIREGARVIMSKTTIQTFLNLAKKTN"
     gene            complement(4884..5798)
                     /locus_tag="PKM27_18235"
     CDS             complement(4884..5798)
                     /locus_tag="PKM27_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR09265.1"
                     /translation="MDKWHWGIDLGGTKIEGVIFKILDRKPEILVRKRIPTESQGGYD
                     HILNRISLLLEELGKESGMQPNRLGIGTPGTYDPVTFIHKNSNTTCLNGKPFKSDLET
                     RLGLPLFMANDANCFAVAEATLGAVPQQVPGARVVFGIIMGTGVGGGVVVDGRVIHGR
                     HGIGGEWGHIRTEENDIVCYCGHTGCNENVMSGPALERYYQGLSGKFLPMKEIVQAWR
                     QGPDPHAQATRDRLLSNFGLAASYIVNVLDPDAIVIGGGLGQIDELYESGQGEILKHM
                     FNDRCETPLLRPLLGDSAGVIGAALLAR"
     gene            complement(5773..7722)
                     /gene="glgB"
                     /locus_tag="PKM27_18240"
     CDS             complement(5773..7722)
                     /gene="glgB"
                     /locus_tag="PKM27_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNR09266.1"
                     /translation="MPNRVLPYSRFSDFDIGLFRAGKHFRLYEHMGSHLVEHEGVAGV
                     YFAVYAPAARMVTVVGDFNYWAGDEHLLYVRWDSSGIWEGFIPGMKQGDKYKYRIYSN
                     LYGIVTEKSDPFARHCETPPYTASIVWPIDYTWRDESWMESRQEKQALSAPMSVYEVH
                     LGSWRRPWHEDRPFSYREHIEQLVPYVKEMGFTHVEFLPVMEHPYTPSWGYQITGFFA
                     PTSRFGTPQDFMQLVDAFHEAGIGVILDWVPSHFPEDAHGLGKFDGTAVYEHPDRRKG
                     YHPDWKSLIFNYERNEIRSFLISSALFWLHHYHVDGLRVDAVASMLYLDYSREAGGWE
                     PNIYGGNENLAAISFLKDFNEAVYRDFPGVQTIAEESTSFARVSRPVFMGGLGFGLKW
                     MMGWMNDTLRYFKTDPFFRKYHQNTLTFSMMYAFTENFTLPLSHDEVVHGKASLINKM
                     PGDHWQQFANLRTLFTYMFTHPGSKLLFMGAEIAQYHEWKFDSSLDWHLLSHHSHLGV
                     QSLIRDLNKLYRSEGALFENQYHSEGFEWIDCNDAESSVISYVRKAKDPKDFLLIVCN
                     FTPVPRHDYRIGTPPCKTMEEVFNSDQTRYWGSGQVNAGILKVESVPQHFRSHSLVIN
                     LPPLGVTVFKPLYSTEENGQMALGH"
BASE COUNT         1947 a         2012 c         1942 g         1860 t
ORIGIN      
        1 cctccagtcc gatcgaaaca aaagcaaggg caaaccacag ggtccgcagt gcgtttaaaa
       61 atccactcac ctgtttggtg gtttcaccgc tgagcaggaa agaaaagacc agggatacgg
      121 caataaaacc caggacaaaa acagggaacc gctgccaaat ggtttttaaa cttagccccc
      181 gggcctcctg gaccttgttt ttggagtagg cccaccatcc cgcaatcaaa aaggcggcca
      241 cccccatcaa tacattctgt gaaaatttta cgatgacgcc cgctttggtt gccaggggcc
      301 cgaccagttg ggctgcggcc gtcaccgatg ccgtcgtatc caaagtgccc ccgagccagg
      361 ctccgccgac cagttcggga atattgaaat accggatggc ccagggctgc aggaccagca
      421 tgggaatggc caccaggaga accagggtgg tgatataaga caattttttc ttgtctccct
      481 ggatggcgcc gctcgccaca atggccgccg acacgccgca gatggaaacc gccgaagcca
      541 ggatgacccc gaattcgtca tccaccttca tcttccggca aagccataag gcaaagaacc
      601 aaacgaccag caccacgaga atggcttgca ggataccggg aaggccggct ttgacaatat
      661 cggtaaagag aacactgctc cccaggatca ccaaaccggt tttgataaaa aattcggacc
      721 gggccgcctc cctgagccag gcgggagcca ggttgagatt gctcaataac aaaccggtca
      781 acaaaccaaa aaccacatat tccacgccat aggcattgac cgttttgttg cccgcgagga
      841 ccagggatat acaagccaat aaaaaaagaa ccagaaatcc tgcaaacttc cggaccagaa
      901 accaggccag ggccaggtag aggatcccaa ggatcccaag ggtcgtcagg ttggatgcgg
      961 aaaacaccct cgagcccagt tctcccccat ccttccaagc ataagcaggg ctgacaaact
     1021 tatagtccgg attcaggagt gcaaaaccca ggactccgcc aatcaggacc cccgcgatcc
     1081 aaaccgccca gccatcttca cccagtcttc tgaaataaga gccgggtccg gaggaggaca
     1141 gggattcctg agagaatgat ttatcagtca tggtttataa gcattgaaaa atataaaaat
     1201 agtattctcc ccggaatgag agtccatgtc ctggtcaatc aaatattact ctggaatccg
     1261 gttaaagcaa aactgttata aagatcggta aagggattgc agnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnccgaacca ggtttgttct tcgtgatgga tattcttttg
     1441 agcgcccaat atcccaaagc ataatatcag gcacaagccc gtattcagta tcctcctcat
     1501 accggcatca aaaagcatgc ttcagggtaa gcccaaaagt gagcggatcc cccagcacgc
     1561 tggcgtaaag gcctgcattt ccccctgcgg gcaataattg ttcatgatag tttttgttca
     1621 acagattacg ggaccagata aagatggaag tgcccgtcaa cacccggata cccgcccggg
     1681 cattgagcag ggtgtacccg tcgatgttca ggtaggctga gggagatgga ctggatgaaa
     1741 acccggaccg gtagtaagaa tccagggcaa taaaaaaatg acctgcctta ctcagaaatt
     1801 ttgccggcct ggtatactct ccgcccagtg aagccgccca tctggaaaga ccgggaaggt
     1861 cgccgcctga aacatcctta aacgccacct gcacgccgtc tatggtctgg ccggtttctt
     1921 caaggggaag cggcgcattt ttaaagctga cgtatttccc ttctgtgtag gcaagggccg
     1981 tatagaaaga aaagttattg ttgaccttga tatttccgtc cagttcgact ccgaacaccc
     2041 ttactttttc cgcgttcgcg atataacccc ggttgactcc cagttccggc gactggacat
     2101 tggtttgata atcatcaatg tcggtatggt ggaaagtgag gtttaaagtt gaattttcaa
     2161 ccggcgatgt tttcaggccg atttcaatat gttttaaata ctccggtttg attaccgcaa
     2221 gttcggtcgc aggctttccg cttatggtgg gcaatccggc cacattgacc ccaaccggct
     2281 tgaagctggt ggaataggat gcaaaagcat tgaggcgctt actggctcgg tatgccaggg
     2341 tgaccaggta ggtcagattg gattcatccg catccgcttc ataggattgg ctggtgtaaa
     2401 tcccgttttt taaagcaagc agagccggat cgttggtttg caggccgcca aaggccgtgc
     2461 gcttatagga aacatctttt ttgtcaatgt tgaaacgaag tcccggctgg aggtgcaggc
     2521 cctccagaat ctcccaatcc acattggcaa aggccgctgc gctgatggat tggattgaag
     2581 ccttggtgga tatcccgtat ccttcaaaaa gcccggctgt cttccagagc gggctggttg
     2641 aactttgcga aaacctccac tggtcccggc cggattgctc cgttccattt attttcacct
     2701 cctgatcgat gaaatataat cccaggaccc cgcttaatcg gctgttgact tcccctgcat
     2761 aacgaatttc ctgggaccaa ttcctgtgtt tagccgggtt ttgggatttt tccagggctt
     2821 gcaagccggt aaaatcccgg tcattggaag gatcccaatt ccagtaacgc catgccgtgg
     2881 tggcggtcag agttcccgga cctattttag aatccaggtt gagggataca ccgcccagtt
     2941 cattgcccga attccaggga gtgtcgtgat cgattttccg gtcaaaggcg ttcaggctgg
     3001 gcaaactgta attcaggtct gcaatgatcg cgttgaactg ccggtaggct gcccttttcg
     3061 tcggcgccac acccgcagcc acctgggcat acccatccgg acgctggcgg gatgcatcaa
     3121 ccgcaagcgt cagggaagtg ttttccgagg atttaaacaa cagttggccg cgaactccca
     3181 ggttattctg gtcattgata aatttattgg tacggatatt ttcaatgacc ccgtcccgtt
     3241 gagtgccgga aaaggataaa cggccggcaa atcgattacc caacggaccg gttaccgaag
     3301 ttttggcctg aatgtatcca tagttgccat aactcagttc aaaatccgcc ccggggataa
     3361 aacttgcctt acgggaagtg atgttgaaag cgcctgcggt ggtgtttttc ccgaatagag
     3421 ttccctgcgg tccacgcaat acctcgattt gttcaatatc gataaagtca aaggtcgcgg
     3481 ccgcaggcct ggcataataa acgccgtcta cataaaatcc cactcccggg tcaaggccat
     3541 cattggtgag cccaaagggt gaacccacgc cgcgaatatt aattcccgta ttcctcgggt
     3601 ttgaggtata aagctggacc gacggaatgt attcttttat ccggttcaca ttaaaagctc
     3661 cggcctgttc ggcaaatacc ccggatacca cagatatcgc gacaggaacc tcctgggccg
     3721 tttcctgcct gcggcgtgcg gtgatcacga tctcggcaag gaggttgtct tccgccagga
     3781 tgatttccac cggcgttgac gggatattgg ttatttggat ttcgcttttc ctaaaaccca
     3841 ggaaactggt ttccagggta taaggagttt gaggaaggcc ggaaaggatg aattttccat
     3901 cggcatctgc aatgccgtac cggttggttc ctttaaccaa caccgtcgcc ccctccaggg
     3961 gctcgccgtc agggctttta attacccctt ccagggtggt ctgggcaaaa gagcggcttc
     4021 ccaggcccag aaaaaatata gtaatgatta tgaatgattt cataggattt gaattatgaa
     4081 tagagtgatg aatattggag cggaccttgt tttttcccgc tgcctataaa gaaatagagt
     4141 tgtgaagttt tggggggtct ttcgcttttc agcgtgagtt tatcatccgg ctgccttggt
     4201 ttgcagggat gccgttttta tacaaacgct tccgcaacaa caacggccgt tggtaaacac
     4261 cttcctccag taaacggggt cgaactgttt catcgtccca caaagataga ataattatac
     4321 aaactctatc aacttagtag agttttataa aaaaaattcc gtccggcttt gtctttttca
     4381 agcctacccc tcttgtggtt tttcctcaat ttgtcttttt ggccaggttg aggaaggttt
     4441 gaatggtggt cttgctcatg atcacccggg ccccttcccg gatctttaag aattcgggct
     4501 tcaggaggca aaccttctcg cttttgcagt cgtcgcattt ttcataaaaa ttgagcgata
     4561 cgcagggcac cagggaaatc gccccgtcaa acaggcgata cacgtccgcc acgctgatct
     4621 ccgccggctt tcttttcaaa taatacccgc ctccatatcc ctgtttgctg ccaacaaagc
     4681 cggcatgttt cagttccaac aggatctgct cgagaaattt tttggggatg ttttcggctt
     4741 ccgaaatggt cgatgccatg atatagccct tatcgtaacc cattccaagc tggatcaggg
     4801 cctttagagc gtacttgcat ttcatcgaca tcatatcctg taaaagtaaa aaatttccct
     4861 caggagggcc tgggccaaca ggtctacctg gccaacaggg cggccccgat cactccggcg
     4921 ctgtctccca acaggggccg gagcaagggg gtctcacaac gatcgttgaa catgtgcttg
     4981 agaatctccc cttggccgga ttcatacaat tcatcgattt gtccgagtcc cccgccaatg
     5041 acgatggcat ccgggtccag gacgttgacg atatacgatg cggccaatcc gaaattagac
     5101 agcaggcggt cacgggttgc ctgggcatgg ggatccggcc cttggcgcca ggcctgaaca
     5161 atttccttca tgggtagaaa tttgccgctc aatccctgat aatatctttc cagggccggg
     5221 ccggacatca cattttcatt gcaaccggta tggccgcaat aacaaacgat gtcgttttct
     5281 tcggtccgga tatgccccca ttcccctccg atcccatgcc tgccatggat cacccgccca
     5341 tccaccacca cgcctccccc gacaccggta cccatgatga tcccaaaaac cactcgtgcc
     5401 cctggaacct gttgcggtac ggctccgagc gtcgcttccg ccacggcaaa acaattggcg
     5461 tcattggcca tgaagagagg cagtccgagc ctggtttcca ggtcggactt gaagggcttt
     5521 ccgttgagac aggtggtatt ggaattttta tgaataaagg tgaccgggtc ataggttccc
     5581 ggagtgccga tccctaagcg gttcggttgc atgccgcttt cctttccgag ttcttctaac
     5641 agtaaggata tgcggttcag gatatgatca taacctccct gactttcggt gggtatgcgt
     5701 ttccggacca ggatctccgg tttgcggtca aggattttga atatgactcc ttcgatttta
     5761 gtgcctccca ggtcaatgcc ccaatgccat ttgtccattt tcttccgttg aatacaaggg
     5821 tttgaaaaca gtgactccca aaggtggcag gttgatcacc agggaatggc tgcggaaatg
     5881 ttgaggtaca gattccactt tgagtatccc tgcattgacc tgcccgcttc cccaatagcg
     5941 ggtttggtcc gaattgaaga cttcttccat ggttttgcag ggaggggttc caatccggta
     6001 atcgtgccgc ggcaccggcg taaaattgca cacgatcagc agaaaatctt tggggtcctt
     6061 ggccttgcgc acataggaaa tgaccgaaga ctctgcatcg ttgcaatcga tccattcaaa
     6121 accttcggaa tggtattggt tttcgaaaag ggccccttcc gaccggtaca gtttattgag
     6181 gtccctgatc agcgactgga cgccaagatg cgaatgatgg gacaacaaat gccagtcgag
     6241 gctgctgtcg aatttccatt cgtgatactg ggcaatttcc gcgcccatga acagcaattt
     6301 ggaacccgga tgggtaaaca tataagtgaa cagggtcctc aggttggcaa actgctgcca
     6361 atggtccccg ggcattttat tgatgagcga agctttgcca tggaccactt catcgtggga
     6421 aaggggaagg gtaaagtttt ccgtgaaagc atacatcatg ctgaacgtaa gcgtattctg
     6481 atggtatttc cggaaaaagg gatcggtctt gaaatacctg agggtgtcat tcatccagcc
     6541 catcatccat ttcaggccga aacccaaacc gcccatgaat acggggcgcg ataccctggc
     6601 gaaggaagtg gactcttcgg caatggtttg cacacccgga aaatcccggt aaacggcttc
     6661 attaaaatct ttcaggaagg agatggcggc caggttttca tttcccccgt aaatattggg
     6721 ttcccagcct ccggcttccc tggaataatc aagatacaac atggaggcca ccgcatccac
     6781 acgcaatccg tccacatgat aatgatgcag ccagaaaagg gcgctggaaa tcaggaaaga
     6841 ccggatctca ttgcgttcat aattaaagat caggcttttc cagtccggat gatacccttt
     6901 gcgacggtcg gggtgttcat acaccgcagt gccgtcaaat ttacccaggc cgtgcgcatc
     6961 ctcgggaaaa tgagaaggga cccagtccag gatcactccg atcccggcct catgaaaagc
     7021 gtcgaccagt tgcataaaat cctggggagt cccgaaacgg ctggtcggtg caaaaaaacc
     7081 ggtgatctgg taaccccagc tcggcgtgta aggatgttcc atgacgggca ggaattcgac
     7141 atgggtaaag cccatttcct ttacataagg aaccagttgt tcaatatgct cccggtaaga
     7201 aaaggggcgg tcttcgtgcc agggccttct ccagcttccc aggtgcactt cgtaaacgct
     7261 catgggtgcg gacagagctt gtttttcctg cctggattcc atccacgatt catccctcca
     7321 ggtataatca atgggccata cgatcgaagc ggtataagga ggggtttcac aatgcctggc
     7381 aaaaggatcg cttttttcag tgacgatccc ataaaggttt gaatagatcc tgtatttgta
     7441 tttatccccc tgtttcatac cggggataaa accttcccaa atccccgagg aatcccacct
     7501 cacatataac aaatgttcat cgccggccca atagttaaag tccccgacga ccgtgaccat
     7561 cctggcggcc ggggcataga ccgcaaaata aacgccggcc acgccttcgt gctcgacgag
     7621 atgggaaccc atatgctcat acaacctgaa atgtttgccc gccctgaata atccgatgtc
     7681 aaaatctgaa aaacggctgt aaggaaggac acggtttggc atgggtccaa ggtaaggaaa
     7741 agttgaggta tgaggttagg ggtaggaggt tagaggtacg aggttagagt acacctctgt
     7801 cttagcgaga gtattcagaa aagtgtgtca aagtttaaaa ttgatttatt aacaaattaa
     7861 a
//
LOCUS       DAOQXT010000162         7818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_9743_length_7818_cov_5.272575, whole genome shotgun sequence.
ACCESSION   DAOQXT010000162 DAOQXT010000000
VERSION     DAOQXT010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9743_length_7818_cov_5.272575"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /locus_tag="PKM27_18245"
     CDS             <1..1771
                     /locus_tag="PKM27_18245"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNR09267.1"
                     /translation="AIISSAALLNALLVVGKKIEDVRIVVSGSGASAISCTRLYIKLG
                     ARRENIVMLDSTGVLNRKRTDLNKQKQEFVTARDIDTLAEALTDADVFLGLSVAGVLK
                     KEMLLSMAKDPIVFALANPDPEITYEDATSTRDDVIMATGRSDYPNQVNNVLGFPYIF
                     RGALDVRATVINDEMKLAAALALANLAKLPVPEEVNSAYGGSNLHFGPEYIIPKPVDP
                     RLITTVAIAVAKTAIATGVARKTIEDWEAYGEELTKRLGLYKPLIRQIRSKARSNPKR
                     VVFAEAESYKILKAAEICIEEKLAKPILLGNEENIKKKIEEFDLSLENAVIIDPRSEN
                     QQERRARFANTYFNKRNRKGVTLRSAQNTMFEWNYFGLSLIEHGEADAMVSGLTANYP
                     EFMRPALRLFGQAQNVHIVAGMDILMTKRGPLFFADTMVNLDPDARTIVNITLQTADM
                     VRRFNIVPKIALLSYSNFGSVKGVVPTKMREAVSILHRDYPDLLVDGDIQGNFAINSE
                     MVRETFPFSKLVDGPANTLIFPNLASAHIAFKLLKEGGNLDSIGPVLTGLKKSVHVLS
                     LGSTVTEIVNMVSIAVADAQDKA"
     gene            1815..2894
                     /gene="aroC"
                     /locus_tag="PKM27_18250"
     CDS             1815..2894
                     /gene="aroC"
                     /locus_tag="PKM27_18250"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNR09268.1"
                     /translation="MAGNQFGEIFRISTFGESHGKAVGVIIDGCPAGLLIQSDFIQKE
                     MDRRRPGQSAIVTQRKEADEIEILSGVFENRTTGAPISMMLRNVDQHSADYDHIKDKF
                     RPSHADFTYQNKWGLRDHRGGGRSSARETAARVAAGAVAKLFLHQLDIHIHGFVSQVG
                     PIRYEPDLDRFDPAEVEANPVRCPDPETAEKMIELIREVRKDGDTIGGVVTCLVRGCP
                     PGLGEPAFDKLHACLGHAMLSINACKGFEIGSGFGGVSMRGSRHNDVFYKEGDDIRTR
                     SNHSGGVQGGISNGMDIYFRVAFKPVATIVPAQESVDLEGNSVTVTGKGRHDPCVVPR
                     AVPIVEAMTALVLADHVLRHRASRI"
     gene            complement(2963..>3387)
                     /locus_tag="PKM27_18255"
     CDS             complement(2963..>3387)
                     /locus_tag="PKM27_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNR09269.1"
                     /translation="DMLRIKSGGSLRPPSEETVAHREHFVWQDGRVVFKAAVKGMAEA
                     VKEVISRAGYQSADIDWIVPHQANYRIIKSVAEMLDYPLEKVMINIQKYGNTTAATIP
                     LCLWEYEPQLKEGDKLVLTAFGGGYTWGAILLTWAYQK"
     assembly_gap    3388..3487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3488..4116)
                     /locus_tag="PKM27_18260"
     CDS             complement(<3488..4116)
                     /locus_tag="PKM27_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNR09270.1"
                     /translation="MSGDITVAITGVHAFVPEDVLTNKDLEKLVDTNDAWIMERVGIK
                     ERHILKDPDKASAFMGAEAVKGLLQKTGTDPEDVELLICATVTGDYQVPDTANQICKL
                     SGLSRAYGFDLNAACSGFLFALATGAQFIKSGTHRKVIVVGSDKMSSITDYTDRSTCI
                     LFGDAAGAVLLEPSINEFGLEQIALYGDAAGKDMLRIKSGGSLRPPSEET"
     gene            4313..5263
                     /locus_tag="PKM27_18265"
     CDS             4313..5263
                     /locus_tag="PKM27_18265"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR09271.1"
                     /translation="MKATKSLFILLAGLAFTLPAQAEDSVVFYKGTVEGAREKARKES
                     KYYFIEFYAKWCEPCKWMDEYTYKNEELSDYVSQHYVPVKVDVENLDGYVWKQKFKVQ
                     YLPTIIVLNSNGTMLGKYEKSLTAPDLIRILKNHRSLPGEAGPAQLSNETDGVVAVPV
                     KTNQENAAYPAFIEVNREPGLPKANEVNNPAKKAAVKPATNPPSANPARAASNPATVA
                     RRPVTSPASKAGTGEEYRIQVGVYSDATNVFNQVNQLKKTFSQVVQVFNSKNKETNSV
                     SYRITLGKFDSREEAAQFAQTLKAKGITGVIKHIRELEQK"
     gene            5504..>7818
                     /locus_tag="PKM27_18270"
     CDS             5504..>7818
                     /locus_tag="PKM27_18270"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09272.1"
                     /translation="MNFTRKAQLAIVFLFGILSTQAQRPEVVFIEGNARLSNQRRALG
                     CPGAEAGTITITPNANNKSSNIQYLCYGDRLNIVHNRNAVFQDPKPLTTPGTGYVFYN
                     CRPTVDGPTLTAVNQDPCVNRTSPIIVNGFPVHQLQGLWLAKGNNGIGDIEFNNDGFL
                     QNAYNGGKPVQFWFAPITLDQFSTQSGVPQFETDANNIPGPCIDVNIDAAFSVVYLNE
                     VLVSDITGVNGGPGNYTGTLTITGGLPEFDPNANYPTVHIENTQDPNILGMLTNGPAT
                     HGKPMTFSVPQPGRYFITVEDGNGCPTERYTVNIPADDGLLKLMCQGGPIGQEICIPI
                     NISKMPELAAGQFTLLFNPQAFDFVGARNLNPTLNTSPENVIVDNQKVAGILKFLWFD
                     LNLAAHDFSMETRLVEFCFRPKGPPGNYDFRITGSPVALEFADIGGNPYPLNPGSGSV
                     LTCASMISPASNLDAYYSQCGQTMFVSVFAAQTPISVKWEQEGVPGNTRIINLLAAGG
                     RDTLFSNQPPARYFISVTDNTGINIRDTITFSGGIAPLTLDLTTRENASCKNNDGRVD
                     PQVLGGTPGYRYEWSNGSVDAVQTRLSVGTYRLTVTDAQGCKLEDSVRINQSIVKAEI
                     TIQTRPTCPGIDNGVLQPNFSAITGIPPFTVIWEGQGTQIGNAFRNVGTRSTRLIVTD
                     FRGCSDTAVIISTAQKQINIVAEVDNPRCFGNADGRLRISRSFSDGSQMTNEMVRLTR
                     YSSGITSNDALRPAIFNDLNADRYNILVTDAS"
BASE COUNT         1937 a         2086 c         1948 g         1747 t
ORIGIN      
        1 ggccatcatc tcctcggcgg ccctgctcaa cgccctcctg gtggttggaa aaaaaatcga
       61 agacgtccgg atcgtagtga gcggttccgg cgcctccgcc atctcctgca cccgcctgta
      121 tatcaagctg ggcgcccggc gcgaaaacat cgtaatgctg gacagtaccg gagtgctcaa
      181 ccgcaaaaga accgacctca ataaacaaaa acaggaattt gtcaccgccc gggacatcga
      241 taccctcgcg gaagccctta cggatgccga cgtcttcctg ggcctgagcg tggccggggt
      301 cctgaaaaaa gagatgctgc tgtccatggc caaggacccc atcgtatttg ccctggcaaa
      361 cccggacccg gaaatcacct acgaagacgc aaccagtacc cgggatgacg tgatcatggc
      421 caccgggagg tcggactatc ccaaccaggt caataacgtc ctcggttttc cctatatttt
      481 ccggggagcg ctcgacgtcc gggctaccgt catcaacgac gaaatgaaac tggccgcggc
      541 cctggccctg gccaacctgg ccaaactccc ggtacccgaa gaagtgaatt ccgcctatgg
      601 gggatccaac cttcatttcg gccctgaata catcatcccc aagccggtcg accccaggct
      661 gatcaccacg gtggcgatcg ccgtggccaa aaccgccatt gcgaccggag tggcccgcaa
      721 aaccatcgag gattgggaag cttacggaga agagctcacc aagagactgg gcctttacaa
      781 acccctcatc cgccagatcc ggtcgaaagc caggtccaat cccaaaaggg tggtctttgc
      841 cgaggccgaa tcctacaaga tcctgaaagc cgccgagatc tgtatagaag aaaaactggc
      901 caaacccatc ctcctgggca atgaagaaaa cattaaaaag aaaattgagg agtttgacct
      961 ctccctggaa aatgcggtga tcatagatcc ccgctcggaa aaccagcagg agagaagggc
     1021 ccggtttgcc aacacctatt ttaacaaacg caaccggaag ggggtcaccc tccggtccgc
     1081 gcaaaacacc atgtttgaat ggaattattt cgggctcagc ctgattgaac acggggaggc
     1141 ggatgccatg gtcagcgggc ttacggccaa ttacccggaa ttcatgcggc cggccctccg
     1201 gctgtttggc caggcccaaa acgtgcatat cgtggcgggc atggatatcc tcatgaccaa
     1261 aagaggtccg ctattttttg cggacaccat ggtcaacctc gatcccgatg cacggaccat
     1321 tgtaaacatc accctgcaaa ctgccgacat ggtgcgccgg tttaatatcg tgcccaagat
     1381 cgccctgctc tcctacagca attttggttc ggtgaaaggc gtcgtgccca caaagatgag
     1441 ggaagccgtg tccatcctgc accgggatta tcccgatctg ctggtggacg gggatatcca
     1501 gggcaatttt gccataaaca gcgaaatggt ccgggagacc ttcccctttt caaaactggt
     1561 ggatggtccg gccaataccc tgattttccc caacctggcc tccgcccata tcgcatttaa
     1621 attgttaaag gaagggggga atctcgattc catcggcccg gtgctcacgg gtttgaaaaa
     1681 atcggtgcat gtcctgtccc tgggcagtac ggtgaccgag atcgtcaata tggtctccat
     1741 cgccgtggcc gacgcccagg acaaagcctg accgcatctg ggccggactg cgtattttta
     1801 ccagtaaaaa gcaggtggcc ggtaatcagt tcggagaaat attcaggatc agcacttttg
     1861 gcgaatccca cgggaaggcg gtcggcgtca tcatcgacgg atgtccggcc ggtttgctga
     1921 tccagtcgga ttttatacaa aaagaaatgg accgccggag gccggggcag tccgccatcg
     1981 tcacccaacg caaggaagcc gacgaaattg aaattctgtc gggggttttt gaaaaccgta
     2041 ccaccggcgc ccccatcagc atgatgctcc gcaatgtgga ccagcattcc gcggactatg
     2101 atcatatcaa agacaaattc cgtccgtccc atgctgattt tacctatcag aacaaatggg
     2161 gccttcgcga ccaccggggc gggggtcgtt cctccgcccg cgagaccgcc gccagggttg
     2221 ccgcgggggc ggttgccaaa ttgtttttac accagcttga tattcatatc catggattcg
     2281 tcagccaggt gggccctatc cgttatgaac cggatctgga ccgtttcgat cccgcagagg
     2341 tggaagccaa tcctgttcgt tgtcccgacc cggagactgc agagaagatg atcgagctga
     2401 tccgggaggt gcgcaaggat ggggatacga tcggcggggt agtgacctgc ctggtcaggg
     2461 gctgcccgcc gggcctgggc gagccagcct tcgacaaatt gcatgcctgc ctgggccatg
     2521 ccatgctcag catcaatgcc tgcaaaggtt ttgaaatcgg gtccgggttt ggaggggtaa
     2581 gcatgcgggg ctcccggcat aacgatgttt tttataagga aggggatgac atccgcaccc
     2641 ggagcaacca ttccggggga gtccagggcg gcatatccaa cggcatggat atctatttcc
     2701 gggttgcttt caaacccgtg gccaccatcg tcccggcaca ggaatccgtt gatctcgaag
     2761 gaaattccgt caccgtcacc ggcaagggca ggcacgatcc ctgcgtggtc ccccgagccg
     2821 tgccgattgt ggaagccatg acggccctgg tactggccga ccacgtgctg agacatcggg
     2881 catccagaat ttaattaaga attaaaaaat taagaattaa gggcagggat ctgctttaat
     2941 ttattaattc ttaattcctt aattatttct gataggccca ggtcaaaagg atcgctcccc
     3001 aggtatatcc tcccccgaag gcggtaagca cgagtttatc cccttctttt aattggggtt
     3061 cgtattccca aaggcagagg ggtatggtgg cggccgtggt gtttccgtat ttctggatat
     3121 tgatcatgac tttttccagg gggtagtcga gcatttcggc cacggatttg atgatccggt
     3181 aattggcctg gtggggaacg atccagtcaa tatcggcgga ttgatagccc gcccggctga
     3241 tgacttcttt gacggcttcg gccatgcctt tcaccgcggc cttaaaaacg acccgcccgt
     3301 cctgccagac aaagtgctcg cgatgggcga cggtttcctc tgaaggcgga cgcagggatc
     3361 ctccggattt gatgcgaagc atgtcttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnngtt tcctctgaag gcggacgcag ggatcctccg gatttgatgc gaagcatgtc
     3541 ttttccggcc gcgtcgccat aaagggcgat ttgttcaagg ccgaattcat tgatggacgg
     3601 ctccagtaac acggcgccgg cggcatcccc aaagaggatg caggtggacc ggtcggtata
     3661 gtcggtgatg gaggacattt tatcgctgcc caccacgatc actttccggt gggtaccgga
     3721 tttgataaac tgggcgccgg tcgcgagggc aaataaaaat ccggaacagg ccgcattgag
     3781 gtcaaaacca taagcccggc tcagtccgct gagtttgcag atctggttgg ctgtgtcggg
     3841 cacctggtaa tccccggtga cggtggcgca aatcagcaat tccacgtctt ccggatccgt
     3901 gcccgttttt tggagaaggc ccttgacggc ttccgctccc atgaaggcgc ttgccttgtc
     3961 cggatctttg aggatatgcc tttctttgat gccgacccgt tccatgatcc aggcgtcgtt
     4021 ggtatctacc agcttttcaa ggtctttgtt ggtcagcacg tcttcaggta caaaggcatg
     4081 cacgccggtg atggccaccg taatgtctcc actcatggcg caaatgtata aaattttcgg
     4141 attcaggagg agtaagccaa gaatcatata aaatattttt attactcata gtagtataaa
     4201 aatcaatgaa atacagtttt attaaatatc gtataactaa atctggtatg ggaattgctt
     4261 tatttatacc gttgcacaga actattaatt aacctaaatt ctggaggtaa ctatgaaagc
     4321 gacaaaaagc ctgtttatcc tgctggccgg cctggccttc acacttcccg cccaagcgga
     4381 agactcggtg gtcttttata agggcacggt tgaaggagcc cgggagaaag cccgtaaaga
     4441 atcaaaatac tatttcattg agttttatgc caaatggtgt gaaccctgca aatggatgga
     4501 cgaatacacc tataaaaatg aggaattgtc cgattatgtt tcccagcact acgtcccggt
     4561 aaaagtcgat gtggaaaacc tggacggcta tgtctggaaa caaaaattca aagtccagta
     4621 tctgcctacc atcatcgtac tgaactccaa cggcacgatg ctgggtaaat atgaaaaaag
     4681 cctcacggct cctgacctga tcagaatatt aaagaatcac cgatccttgc ctggggaggc
     4741 tggtccagcc caactgtcca atgaaacaga tggggttgta gctgtgcctg tcaaaaccaa
     4801 ccaggagaat gccgcttatc ccgccttcat cgaggtgaac cgggagcccg gccttcccaa
     4861 ggcaaatgag gtgaacaatc ccgcaaaaaa agcggcggta aaacccgcca caaacccgcc
     4921 atcagcaaac cccgcaagag cggcctccaa tcctgctacc gttgccagac ggccggttac
     4981 aagcccggcc tccaaagcgg gcaccggcga ggaataccgc atccaggtgg gcgtttactc
     5041 cgatgccacc aatgtcttca accaggtcaa tcaactgaaa aaaacctttt cccaggtagt
     5101 ccaggtcttt aattcaaaaa acaaggaaac caactccgtt tcttaccgca ttacactggg
     5161 caaattcgat tcccgggaag aagccgccca atttgcccaa accctgaagg caaaagggat
     5221 tacgggggtg atcaaacata tccgggaact ggagcagaag tagtgaatgg tggatagtga
     5281 atggtggatg gtggttggtg gcagccaact tgctacctta ctaacttgca aactaactaa
     5341 ctagcaaact aactaactaa ctacattaat cttctgtgaa tcaatactaa tcccgcattt
     5401 cagaccgttc attcgataat tgtgttaaaa ttgaaccgga tgatgggcct tgcttaaatt
     5461 tgcagctcga atcccataag ccgattcaat aatttataat ccgatgaatt tcaccagaaa
     5521 agcgcagtta gcaatcgttt ttttatttgg cattttaagt acacaggctc aacggccaga
     5581 ggtggttttc atcgagggca atgcccgtct ttcaaatcaa aggagggctt tgggttgtcc
     5641 cggcgcagaa gccggaacca ttaccatcac tccgaacgca aacaacaaat ccagcaatat
     5701 ccaatacctg tgctatggcg accggttgaa tattgtacac aaccggaacg cggtattcca
     5761 ggacccgaaa ccattgacta ccccgggtac aggatatgtt ttttataatt gcagacctac
     5821 tgtggatggt cctaccctca ccgcggtcaa ccaggacccc tgtgtaaacc ggacctcacc
     5881 cattattgtc aacggattcc cggttcacca attgcaggga ttgtggctgg ctaaaggcaa
     5941 caatggaatc ggtgatattg aattcaataa cgatggtttt ctgcaaaacg cctacaacgg
     6001 cggcaaaccg gttcaattct ggttcgcccc gatcaccctc gatcagttca gcacccagtc
     6061 cggagtaccc caatttgaaa cggatgccaa caacattccc gggccctgta tcgacgtaaa
     6121 cattgacgcg gccttttcgg tggtctacct taatgaagtc ctggtatcgg acatcaccgg
     6181 ggtcaacggg ggcccgggca attacaccgg caccctgacc atcaccggcg gtttgcccga
     6241 atttgatccc aacgcgaatt atccgaccgt tcacatcgaa aatacccagg accccaatat
     6301 cctgggtatg cttaccaatg gtcctgcgac ccatggcaaa ccaatgacct tttccgttcc
     6361 ccaacccggc aggtatttta tcacggtaga agacggcaat ggttgtccca cggagcgata
     6421 taccgtaaat atcccggcag atgacggcct tttaaaactt atgtgtcagg gtggtcctat
     6481 cggccaggaa atctgcattc ctattaatat ttcgaaaatg ccggagcttg cagccggcca
     6541 gtttaccctg ctgtttaatc cgcaggcctt cgattttgtt ggggcccgca acctcaatcc
     6601 taccctgaat acctccccgg aaaatgtgat tgtcgacaac cagaaggttg cagggatatt
     6661 aaaatttctc tggttcgacc tgaacctggc agcccatgat tttagtatgg aaacccggtt
     6721 ggtggagttt tgtttccggc ccaaaggccc tccgggcaat tacgatttca ggatcaccgg
     6781 atctcccgtt gcccttgaat ttgccgatat cggaggcaat ccataccctc tcaaccccgg
     6841 cagcggcagc gtcctaacct gcgctagtat gatttctccc gcttcaaacc tcgacgctta
     6901 ttattcccag tgcggacaaa ccatgtttgt ttccgtattc gcggcccaaa ccccgatatc
     6961 ggtaaaatgg gaacaggagg gagtacctgg aaatacccgg atcatcaatc tcctggcagc
     7021 cgggggaaga gatactcttt ttagcaatca acctcccgca agatatttta tctccgtcac
     7081 ggataataca ggaataaata tcagggatac catcaccttc agtggaggca ttgctccgct
     7141 cacccttgac ctgacgacca gggaaaatgc cagctgcaaa aacaacgatg gccgggtgga
     7201 tcctcaggtc cttggcggta ctccaggata ccgttatgaa tggtccaatg gttctgtgga
     7261 tgctgttcaa acccgcttga gtgtaggtac ttaccggctc accgtcacag atgcacaagg
     7321 gtgtaaactt gaggacagtg tcaggatcaa tcaatccatt gtaaaagcag aaataaccat
     7381 acaaacccgc cctacctgcc cggggatcga taatggtgtc ctccaaccca atttttccgc
     7441 cattactgga atacccccct tcactgtgat ttgggaagga caaggaacac aaataggcaa
     7501 tgccttcagg aatgtgggca caaggtccac ccgtctcatc gtgaccgatt tcaggggttg
     7561 ttcggatacg gcagtaatca tatctaccgc tcaaaaacaa ataaatatcg tggccgaggt
     7621 ggacaacccc cgttgttttg gaaatgccga tggccgtctc cgtatttccc gatctttttc
     7681 ggatggcagc caaatgacca atgagatggt caggcttacc cggtattcaa gcgggatcac
     7741 cagcaacgat gccttaaggc ctgccatttt taatgactta aatgctgacc ggtacaatat
     7801 tcttgtgacc gatgcctc
//
LOCUS       DAOQXT010000163         7589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_10082_length_7589_cov_5.917308, whole genome shotgun sequence.
ACCESSION   DAOQXT010000163 DAOQXT010000000
VERSION     DAOQXT010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10082_length_7589_cov_5.917308"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKM27_18275"
     CDS             <1..203
                     /locus_tag="PKM27_18275"
                     /inference="COORDINATES: protein motif:HMM:NF021926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HNR09273.1"
                     /translation="AVIMFSCISRHLSFGVMTGEEIDRVIQVWKAPFIGFFSYGEFGK
                     SRRGKHEFHNNTCCVVTLKEIR"
     gene            203..1351
                     /locus_tag="PKM27_18280"
     CDS             203..1351
                     /locus_tag="PKM27_18280"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNR09274.1"
                     /translation="MKAKSIKGKSPQEIRTTLGNARSDGFKPTLAFVFISIQNDLKAV
                     SELLDKEGIQIFGATTGGEFIDGNIESGSVAILLLDMKPEYFALLLEDYRDRDPEAVA
                     RQMSAKAKETFKNPSFIISASINARENSEALGEPLIKAMESVTGKETNIWGGRAGDDF
                     VFNDTVVFNNNLSTERGILMLVLDGDKMIARGEAASGLKAVGTEKIITKASGNWVYEI
                     DHQPAAEMVLKYLGLKLSQEEAETFFPKEGIMFSVSRDKGDPVIRAVGVFNWKDKSFS
                     TLGSIREGDRIRLTLPPDFELVEELSRNAEIISRQELPGTEALLMFSCVGRLGQFGPL
                     IGDEIEGIRKAIGVPLAGFFTYGEFGRTRNGNNEFHTSTCCWVALKEK"
     gene            1401..2528
                     /locus_tag="PKM27_18285"
     CDS             1401..2528
                     /locus_tag="PKM27_18285"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNR09275.1"
                     /translation="MKAKSIKGKSPEEIRKTLGNAMSDGFKPNLAFVFLTEVRDADAV
                     TALMDAAGIAVFGASTSSKFTEEGIEPDGIVILLLDMNPAYFSIVLKDFKTSGSVYQA
                     ACEVGDAGKNSFDHPAFIISTTDISTPGEEVIKGILDKGGIDVPVIGGGAGEPINFTG
                     IVFTKDAKSSSGIITLILNEDKVDVKGVAVSGWKPVGTEKRITKSNGNWIYTIDDEPA
                     LDVIMKFLGKEMLAVDKSSEIDSYPLQLRRESGKPVMRPVVLWKQEDHSVMLGGPVKE
                     GALFRFSLPPDLEVIDEVIDSTKTIRENDLPQADAMIVFSCIGRLSNLGPLVASEIEG
                     LAAAWGGPMAGFFSLGEYGKLDDTRPEFHGTTVSWVALREK"
     gene            2577..3767
                     /locus_tag="PKM27_18290"
     CDS             2577..3767
                     /locus_tag="PKM27_18290"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNR09276.1"
                     /translation="MKAKTIKGKSINEIQPALDQCMTGRFKPTLAIVFMSVKQDREAL
                     SRLFDEKGIQIFGATTAGEFMDGEIEEGSMVAMLLDLNPAYFKLEFIKTSKQTALEDA
                     KKLGEAGKEAFKNSAFIIATGGVDLGGEEIVEGIVEGYRNAGSRLDKEVTLFGGKAGD
                     DLGLEATFVFTNGKSSDHALVALILDEDKMDVRGITTCGWKAIGTTKTVTKSQANIVY
                     TIDDKPALDTLLNYLGVEIKQEGDKALVTFLSSWYYPLQVEQENVEPVIRTAMFANRE
                     DRSLICSGNVPLGSKIKFSLPPDFDSIEKVVAECKAIKDDAQQQADALIMFSCVSRYL
                     SFGMVMKEELEQVQKIWDAPMAGFFTYGEYGKSLSALQAGEAGKNQYHNNCCCLVAIR
                     EKET"
     gene            3827..4966
                     /locus_tag="PKM27_18295"
     CDS             3827..4966
                     /locus_tag="PKM27_18295"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNR09277.1"
                     /translation="MQSKSIKGRSAEDIQNALLQSMADGFKPTLAIVFLSVSQDRKAV
                     RDILDKAGIRIFGATTNGEFIDENPDKFSTAILLLDMDPGHFRILFSEYREKNYREVA
                     RALAAETKRFFHKPAFLISGTHLETDAEQLLLGFEDEVGKEVNVFGGMAGDDYTFSDQ
                     FVFTSDRETGKGMVVLALDEDKIKIRGRATCGWKAVGTEKTVTRSEGNHVFTVDDVPV
                     LDLTSKYGGLENVTPENKDLLLEIAANLPLQLQRPVGDPVMRPGLVVDWKDRSFYCSG
                     SVPQGSKVRFSVPPDFDVMEKVIRGVQELKQTQMPEADAVVVFSCAGRILSLGPLMNQ
                     EIEGVKKVWNVPMVGMFSNAELARATGGNLEMHNLTTCVVALKEK"
     gene            4967..>7589
                     /locus_tag="PKM27_18300"
     CDS             4967..>7589
                     /locus_tag="PKM27_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR09278.1"
                     /translation="MNQSLSHISAAVEGSETLSAEQKKSILDWIKEVDKYLSIVEFKL
                     DRTEKVKRTTAILLEETIQELEQKRKAVEAQNRELEIESSLERVRTAAMSMKKPDDLL
                     FICDVLFKELKSLGFTELRNTLIHTYVDEESYFIDYDYSDYTGGGISHIPYKGVPFIE
                     NYIFEIRKSKEAFTEMVVTGEELEVFKEFRKTTGQLEDLRLDHASALYYYNYSIGVGD
                     VGISTFSPISEEKREVLKRFRNVFDFAYRRYMDVAQAEMQAREATIEASLEKVRGEAM
                     AMHNSSDLSSTILLAYSELKKLGFTFLHFGVGLINKETRKAQWYSVNPIQSGDDLAMT
                     SGILFEGHPVFEAMYDSWIKLEDYFPVLEGEPLTSYYDLLTKGLSVPSLPMGQTGEKK
                     YGLFSPHPVSCLYAWSENAFKEAEIKVLKRFASIIELTFRRYLELQQSEAGAREAVKQ
                     SSLDRVRAEIASMRTVEDLNSITPIVWRELNILGVPFFRCGVFIINVEERLVHVYLST
                     PEGKSLAVLHLGFDGAEITINSVNHWLQQKVYIEHWKREQFMAWVQSLKVQGQVESEQ
                     TYQAGQEPPETMTLQFVPFAQGMLYIGSTEPLNEDQLQLVKELSNAFSVAFARYEDFK
                     KLEEAKTKIETTLVELKAAQAQLIQSEKMASLGELTAGIAHEIQNPLNFVNNFAEINR
                     ELIEEIREERLMLKAEGGKTTEDLILETMKENQDKILQHGKRADAIVKSMLEHSRVHS
                     RKKEPTDLNALCEEYLRLAFHGYRAKDRDFNAHLELNLDPDLPHVNVLPQDIGRVLLN
                     LINNAFYAVQELNKSKKTKGELYSPTVAVTTKSVEKAIEIRVKDNGPGIPQEVVGKIF
                     QPFFTTKPTGQGTGLGLS"
BASE COUNT         2199 a         1546 c         2020 g         1824 t
ORIGIN      
        1 atgcggtcat tatgttttcc tgcatcagcc gtcatctgtc atttggggta atgaccggtg
       61 aagaaattga cagagtgata caggtatgga aggccccttt catcggcttt ttcagttatg
      121 gtgaatttgg aaaatcccga agaggaaaac atgagtttca caacaatacc tgttgtgtgg
      181 tgacgctaaa agaaatacga taatgaaagc aaaatcaata aaaggaaagt caccgcaaga
      241 aatcaggact acgctgggaa acgctaggtc ggatgggttc aaacccaccc tggcttttgt
      301 atttatttcc atccaaaacg acctgaaagc cgtgtcggag ttgctcgata aagagggcat
      361 tcaaatattt ggcgcaacga ccggcggaga atttattgat gggaatattg agagcgggtc
      421 ggtcgctatt ctcctattgg atatgaagcc ggaatacttc gcccttctgc ttgaggatta
      481 ccgggataga gatcccgaag cggtggcaag acaaatgtct gccaaagcaa aagaaacctt
      541 taaaaacccg tcttttatca tctcagccag tatcaatgcc cgggaaaact cggaggccct
      601 gggagaaccg ctgatcaaag ccatggaatc ggtgaccgga aaggaaacaa atatctgggg
      661 aggccgtgca ggggatgatt ttgtttttaa tgatacggtg gtattcaata ataatctgtc
      721 aacggaaaga ggaatcctca tgctggtatt ggatggggat aaaatgatcg caagaggcga
      781 agcggcttcg ggattaaagg cggtgggaac tgaaaaaatc attaccaaag ccagcggcaa
      841 ctgggtatac gagattgacc accaacccgc agcggaaatg gtgttaaagt acctgggtct
      901 taaattaagc caggaagaag cagaaacctt tttccccaag gaaggcatca tgttcagtgt
      961 ttcccgcgat aagggggatc cggtgatcag ggctgtgggg gtgttcaact ggaaagacaa
     1021 atcgttctcc acgcttggca gtatccggga aggagaccgg attcggctga cgcttcctcc
     1081 tgattttgaa ttggtggaag aattgagcag gaatgctgaa ataataagcc ggcaagaact
     1141 accgggaacc gaagccttgc tgatgttttc ctgtgtgggc cggctggggc aattcgggcc
     1201 attgatagga gatgagatag aaggaatcag aaaggccatt ggggtccccc ttgcgggttt
     1261 tttcacttat ggagaatttg gaaggacccg gaacgggaac aatgaatttc ataccagcac
     1321 ctgttgctgg gtggcgttga aagaaaaata atccttcgcg gtccggcaaa atcacggctc
     1381 aaagggacaa aaaaatatag atgaaagcaa aatcaataaa aggaaaatca ccggaagaaa
     1441 tcaggaaaac gctgggaaac gctatgtcgg atgggttcaa accaaacttg gcgtttgtat
     1501 tcttaaccga ggtgagagat gccgatgccg ttacggccct gatggatgca gcaggcattg
     1561 ccgtattcgg cgccagtacc tcttcaaaat ttacggaaga gggtattgaa ccggatggca
     1621 tcgttatatt attgctggat atgaatccgg cttatttcag cattgtttta aaagatttta
     1681 aaacttctgg ctctgtctat caagcggcct gcgaagtagg ggatgcgggc aaaaatagtt
     1741 ttgatcaccc ggcctttatt atctcgacta cggatataag cactccgggc gaagaagtga
     1801 tcaaaggtat tttagacaaa gggggaatag acgttccggt gattgggggt ggagccggag
     1861 aacctataaa ctttacaggt atcgttttca ccaaggatgc aaaatcaagt tcagggataa
     1921 taacactgat tctgaacgaa gacaaagtgg atgttaaagg agtcgctgta tcaggttgga
     1981 aacctgtcgg gacggaaaaa aggatcacta aatccaatgg aaactggata tataccatcg
     2041 acgatgagcc ggctttggat gtgatcatga agttccttgg aaaagagatg ctggccgtgg
     2101 ataagtcatc agagattgac agttatccgt tgcagctcag gcgggagtcg ggaaaaccgg
     2161 taatgaggcc tgttgtcctt tggaagcagg aggaccattc agttatgctt ggaggacccg
     2221 taaaggaggg tgccctgttc cgcttttccc ttccacccga ccttgaagtg atagacgagg
     2281 tcattgacag cactaaaaca atcagagaga atgatcttcc gcaggcagat gccatgattg
     2341 tgttttcctg cataggtagg ctttccaatt tgggtccttt ggttgcttca gaaattgaag
     2401 gattggcggc agcctggggt ggtccgatgg cgggcttttt ttctctcggt gaatatggta
     2461 aactggatga cacccgcccc gaatttcatg gaaccacggt gagttgggtg gccctgagag
     2521 aaaaataatc ccgggtcagt tcatctgaac atggccagtt aagataaaat ggatatatga
     2581 aagccaaaac gataaagggg aaatcaataa atgaaattca accggctttg gaccaatgca
     2641 tgaccgggag gttcaaaccg actttagcca ttgtattcat gtctgttaaa caggaccggg
     2701 aagccctttc cagattattt gacgaaaagg gcattcagat ctttggagcc accacggcag
     2761 gtgaatttat ggatggagaa attgaagaag gcagcatggt cgcgatgctg cttgacctca
     2821 atccggcgta tttcaaactg gaatttatta aaacaagcaa acagacggcc ctggaagacg
     2881 caaaaaaatt aggggaagcc ggaaaagagg cctttaaaaa ttcagcgttt atcattgcga
     2941 caggcggagt ggacctgggt ggggaagaaa ttgttgaagg tatcgttgaa ggctatagaa
     3001 atgccggttc ccgattggat aaggaagtca ccctgttcgg aggtaaggcg ggagatgatt
     3061 tgggactgga ggccaccttt gtttttacca acggaaagag cagcgaccat gccctggtgg
     3121 ccttgattct ggatgaagat aagatggatg tcagaggcat caccacctgt ggctggaagg
     3181 ctattgggac caccaaaacg gttactaaaa gccaagccaa tattgtatat accattgacg
     3241 ataaaccggc actggacacc ttattgaatt acctgggagt ggagatcaag caggaaggcg
     3301 ataaggccct ggtgaccttc ctgagttcct ggtattatcc attgcaagtg gagcaggaaa
     3361 atgtggaacc cgtcattcga acagccatgt tcgccaaccg ggaagaccgt tctttgattt
     3421 gttccggcaa tgtgcccctg gggtctaaaa taaagttttc gctgccgccg gattttgatt
     3481 ccattgaaaa ggtcgtggcg gaatgtaagg ccataaagga tgatgcacaa cagcaagcgg
     3541 acgccctgat catgttttca tgtgtaagca ggtatttatc ttttgggatg gtgatgaaag
     3601 aagagttgga acaggtacaa aaaatttggg atgcgcccat ggccggcttt tttacctacg
     3661 gtgaatatgg aaaatccctg tctgcattgc aggccgggga agccggtaaa aatcagtacc
     3721 ataacaattg ctgttgcctg gtagcaataa gggaaaaaga aacctgaatc gggtgaactt
     3781 aaaaaaaatc tattcaacaa aaccggatta tttatttcaa taaattatgc aatcgaaatc
     3841 aattaaagga aggtcggcgg aggacattca aaatgcgctg ttgcaaagta tggcggatgg
     3901 ttttaaacct accctggcga tcgtgttcct gtccgtaagc caggacagga aggccgtgag
     3961 ggatatcctg gataaggcag gcatacggat tttcggcgcc accaccaatg gggagtttat
     4021 tgatgaaaac ccggataaat tttctacggc gatcctgtta ttggatatgg atcccggcca
     4081 tttcaggatc ctgttttctg aataccggga aaaaaattac agggaggtgg ccagggccct
     4141 ggctgctgaa acaaaacggt tttttcataa gccggccttc ctgatctcgg gcacccattt
     4201 ggaaacggat gcggaacaat tgttattggg atttgaggat gaagttggga aagaagtcaa
     4261 tgtttttggc ggcatggcgg gcgacgatta taccttcagc gaccaatttg tttttaccag
     4321 tgaccgggaa accgggaagg gcatggttgt gctggctctg gacgaagata aaatcaagat
     4381 cagaggcagg gccacgtgcg gttggaaggc cgtaggcacg gaaaaaaccg tcacccggag
     4441 tgaaggcaat catgtattta ccgtggatga tgtcccggta ttggatctca cctccaaata
     4501 cggcggcctg gaaaatgtga ctccggagaa taaggacctt cttttggaaa ttgcggccaa
     4561 tctccccctg cagttgcaaa gaccggtagg cgatccggtg atgcggcccg gcctggtcgt
     4621 ggactggaaa gaccggtcat tttattgcag cggttcggtc cctcagggct ccaaagtacg
     4681 gttttccgta ccgccggatt tcgatgtcat ggaaaaggta atccggggtg tgcaggaatt
     4741 aaaacagacc caaatgccgg aagcagatgc tgtggtggtc ttcagttgcg ccggccgcat
     4801 tctttccctg gggcccctga tgaaccagga aattgaagga gtgaaaaaag tatggaatgt
     4861 acccatggtg ggcatgtttt ccaatgcgga gttggccagg gcgacgggcg gcaacctgga
     4921 aatgcataac ctcacgacct gcgttgtggc tttaaaggaa aaataaatga accaaagttt
     4981 atcccatatc tcagccgctg tcgaaggaag tgaaacctta tccgctgaac aaaaaaaatc
     5041 catcctggat tggattaagg aagtggataa atacctctcc attgtggaat ttaaactgga
     5101 ccggactgaa aaagtaaaaa ggacgacggc catccttctt gaggaaacga tccaggaact
     5161 ggaacaaaaa agaaaagcag tagaagcgca aaacagggaa ctggaaattg aatcgtccct
     5221 cgaaagggta agaaccgcgg ccatgagcat gaaaaaaccc gatgacttgt tgtttatctg
     5281 tgatgttttg ttcaaagaac ttaagtcgct gggttttacc gaactcagga atacgttgat
     5341 tcatacctat gtcgatgagg aaagttattt tatcgattac gattactccg attataccgg
     5401 tgggggcatt agccacattc cctataaggg ggtgccattt attgaaaact atatttttga
     5461 gatacgcaaa agtaaagaag cgttcacgga aatggtggtc accggtgaag agttggaagt
     5521 ttttaaggaa ttcagaaaaa ccaccggcca gttggaggat ttgcgattag atcatgcttc
     5581 ggccctgtat tattataatt attccatcgg agttggagat gtgggaattt ccacttttag
     5641 cccgatttct gaagagaaac gtgaagtgct gaaacgattc agaaatgtat ttgactttgc
     5701 ctaccggaga tatatggatg tagcccaggc agagatgcag gcgagggagg cgaccatcga
     5761 agcctccctg gaaaaggtcc gcggggaagc catggctatg cacaacagca gtgacctgtc
     5821 ttccaccata ctattagcct attctgaact aaaaaaactg ggttttacct ttctgcactt
     5881 cggggtagga ttgatcaata aggagacccg caaagcccag tggtattcag ttaatccaat
     5941 ccaaagtggg gatgatttgg ccatgaccag tgggattcta tttgaaggac acccggtgtt
     6001 tgaagccatg tatgattcct ggattaaact ggaggactat tttccggtat tggaagggga
     6061 accattaacc tcatattatg acttgctcac caaaggtctt tcagtcccct cacttccaat
     6121 gggtcagaca ggagaaaaaa agtatggact tttttccccc catcctgtca gttgtttata
     6181 cgcctggtca gaaaatgcgt ttaaagaagc tgaaataaag gtgctgaagc gatttgcatc
     6241 catcattgag cttaccttca ggcggtacct cgaacttcaa caatctgagg cgggcgcccg
     6301 ggaagctgta aaacaatctt ccctggaccg ggttcgcgct gagattgctt cgatgcgaac
     6361 ggtcgaggac ctcaattcca tcactcccat agtttggcgc gaactaaata tccttggcgt
     6421 accttttttc cgttgcggcg tctttatcat caatgtggag gagcgactgg tccatgttta
     6481 cctttccaca ccggaaggga aatccctggc ggtgcttcac ctgggttttg atggagccga
     6541 gatcaccatc aattcggtga atcattggct tcagcaaaag gtttatattg aacactggaa
     6601 aagggaacaa ttcatggcct gggtacagtc gctaaaagta caaggccagg tggaatctga
     6661 acagacctat caggctggac aggagccccc ggagacaatg actttacaat ttgttccttt
     6721 cgcccagggc atgttgtata tcggcagtac cgaaccattg aatgaggacc aacttcaatt
     6781 ggtcaaagaa ctgtccaacg ctttttcagt agcttttgcc cgatatgaag atttcaaaaa
     6841 actggaagag gccaaaacca aaattgaaac cactttagtt gaactaaaag cggcacaagc
     6901 ccaattgatc caatctgaaa agatggcctc tctcggtgag ctgaccgccg gcatcgccca
     6961 tgaaattcag aatccgttga atttcgtcaa taatttcgct gaaattaaca gggaactgat
     7021 cgaagaaatc agggaagaaa ggttaatgct taaggctgaa gggggcaaaa ccacagagga
     7081 cctgatattg gaaacgatga aggaaaatca ggataaaatc ctgcagcatg gaaaaagggc
     7141 ggatgcgatt gtcaagtcca tgctggagca ttcgagggtc catagtagaa aaaaagaacc
     7201 tacggacctg aatgcccttt gtgaagagta cctccggctt gcttttcatg gttaccgggc
     7261 taaggacagg gacttcaatg ctcacctgga gctgaacctg gacccggatc tgccccatgt
     7321 gaatgtactt ccccaggata tcgggcgggt attgctcaat ctgatcaaca atgcctttta
     7381 cgccgtgcag gaattgaata agtcaaaaaa aacaaaggga gagctctatt cacctaccgt
     7441 tgcggtgact acaaaatcgg tggaaaaggc catagaaatc agggtgaagg acaacggtcc
     7501 gggcataccc caggaagtgg tgggtaaaat ctttcaaccg ttttttacta ccaaacctac
     7561 agggcaagga accggattgg gattgagtt
//
LOCUS       DAOQXT010000164         7562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_10132_length_7562_cov_6.897296, whole genome shotgun sequence.
ACCESSION   DAOQXT010000164 DAOQXT010000000
VERSION     DAOQXT010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10132_length_7562_cov_6.897296"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..2263)
                     /locus_tag="PKM27_18305"
     CDS             complement(128..2263)
                     /locus_tag="PKM27_18305"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNR09279.1"
                     /translation="MRGSLFAMGIAVLFFVFSAGKKGMPETDGSKEAVILHTVMQVLE
                     QAHFRPQQLNDDFSKAVYKSHLQDLDGSKRFFTQNEIGQLKPFELRLDEEVKGRSLEF
                     HYLAARLFKSSLTRAQGYYEEAIKHNYDFKGNETIELDPDKMQYAKNEAELKAYWKKL
                     IKYEIASKVADRLDEQDKDQSGAPKKSLADLQAEAREKVKDTYDDYFSRLGKLDDKVW
                     FESYLNAITGYFDPHSNYFSPKDKEDFNMQMSGKYEGIGARLGQEKDYVKVTSIIPGG
                     PAAKNKELEVDDLIIRVTQDGAEPKDVIGMRVDEVVTFIRGKKGTGVTVSVKKKDGTS
                     KEIHLVRDEVILEEGWAKSAILEIPGMTDKIGFISLPSFYSDFEDNNGKSCSRDVANE
                     IKKLQSQGVRGIILDLRFNGGGSLTEVVDMVGQFIETGPVVQVKSKSGNPTVMQDRDA
                     SVVYDGPLVVMTNSYSASASEILAAALQDYKRAVVVGSKTSFGKGTVQRFFPLDRVVN
                     GLQAYKPLGEIKVTVQKFYRINGGSTQLRGVQPDIELPDTYKYLEIGEREYDHAMEWT
                     ELPKLSYQQNVFTIDNLDQLRTLSKNRTDTSKVFNLIDENALRLKSLRDQTTASLNLD
                     QYRKDESRRHVESEKFSMLNDRNTRLEPSPSASFTASDLALTDVKTLEQRQEEWFRNI
                     KKDVHLEESVLILQDLIRTNEGKVAKSKN"
     gene            complement(2387..3148)
                     /locus_tag="PKM27_18310"
     CDS             complement(2387..3148)
                     /locus_tag="PKM27_18310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR09280.1"
                     /translation="MKVLIVEDEALLSKRLQNLLQEIRPEYEVCGITRSVRETTDWLK
                     QHPEPDLIFMDIELGDGKSFEIFNKNRVDAPVIFTTAYDEYAIQAFKVNSIDYLLKPI
                     NKEELSIALRKFERTVHASGQGAGKLANLIREMIPRKTEYRERFLVKKGQKWISVEAG
                     NTALFMVSHGVPQLLSRDKQKYILAYTLEEIENQVDPDEFFRANRQFLISRQAIKAVH
                     PWFNSKLKVEMEPDPEEEILVSRERAKAFKTWLGE"
     gene            complement(3145..4191)
                     /locus_tag="PKM27_18315"
     CDS             complement(3145..4191)
                     /locus_tag="PKM27_18315"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNR09281.1"
                     /translation="MNKKAGLKDTWFRLLGIPFIALMAHLIFYNRNESGDQRFGFWGI
                     YFFSLLETFAVWESIRLVILYFRRRFPALNQTPKRIAWTLAGTVPVTVFIRWFNLWIY
                     DKTALWSEYPFPLEAYLEAIFVALLFVIIVTGIYEALYFFMKWKEAVVEAEELKARNL
                     QTQLESLKNQINPHFLFNSLGSLSSLILEDPNKAVQFVDDLSAVYRYLLQANEKISAT
                     LREELKFLKAYFQLFKNRFGDGISIDLRIDEAWMDYKLPTLTLQPLVENAIKHNIILP
                     EKPLKIELSVTPDGILTVSNNLQRKNNIGLSNKVGLRNIMTKYKLMHQKDVEIVETED
                     RFMVHLPLIPGGRA"
     gene            complement(4224..5411)
                     /locus_tag="PKM27_18320"
     CDS             complement(4224..5411)
                     /locus_tag="PKM27_18320"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR09282.1"
                     /translation="MKIYLLFPIWLLVFILLSCTREDIPGNPGSCENLRVFNTNHPMK
                     DSLQAILAKYVAKGLPGAEVMLKNKDGWFFATRGYARLESRSAFEPCTPSWQFSITKT
                     FTAVLVLKQVEKGNLDLDRNIKDYLPSRIWPKVPSVEKITLRMLLNHSSGLVNVTELP
                     EFMVGQLNDPLHQPSPDQMVDMIAGLPLLYEPGTDFTYSNTNYLLMHAILEEISQKSY
                     ATLLREEILEPTILQDAWYGLSAEQTESLGFPDYYFDRYANEQLENCKTWNGALARAS
                     NAFGGLAASPEAVIRFYTALLNGRLVNESSLQEMKKWFTGKASGLAEYGLGLECYHYG
                     GQLQYGHEGDGIGNSTQFIYFPGNDTYLYIQTNVGRQLFGPYLFKITDLKNELCNYVA
                     KFN"
     gene            complement(5420..6007)
                     /locus_tag="PKM27_18325"
     CDS             complement(5420..6007)
                     /locus_tag="PKM27_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09283.1"
                     /translation="MKKMKLSILLAMGTMTLSWLRAQSPASSWQVDLAAGTHSVYAPV
                     KNLKYDPTGLITQFGVYHHTGSRQVFSMGFQFGLSGSKSMGDASFAQLVARFTPVIAK
                     HLELGLGTGIGYRLSGYPGSTLTWDGQDWQKGKKFKGLFQVPLQLSLGYRSVKIQNIR
                     LSPYLAYQVQGVFGYSPDLSFLPLSSYSFGLKINK"
     gene            complement(6688..7317)
                     /gene="rsmG"
                     /locus_tag="PKM27_18330"
     CDS             complement(6688..7317)
                     /gene="rsmG"
                     /locus_tag="PKM27_18330"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNR09284.1"
                     /translation="MPALIEQYFPNLSEIQKSRFDSLEPFYREWNEKINLISRKDMEQ
                     FMLHHVLHSLTLGRFMNFAPGTEILDLGTGGGFPGIPLAILWPEVRFHLIDGTGKKIK
                     VVEAAVQHLRLENVTASHIRVEDLSSQYDYVVARAVTSLADLLRLSRKRIRTRHINAR
                     PNGLIAYKGYPLKEEHKTIKSVPHELFMIHEFFNEEYFRGKCIVYIPVY"
BASE COUNT         1736 a         1850 c         1980 g         1996 t
ORIGIN      
        1 gtttttaagt gtttttcaac cttaaaaacg gtcaacctct gtcaggcatt gacaaccctg
       61 aagcctcggg agagcttggt cttaccaact tactaactta ctaacttggt aacttgccaa
      121 ctaagctcta attcttactc tttgccacct tgccttcatt cgtacggatc aggtcctgga
      181 ggatcagcac gctttcttcg agatgcacat cctttttaat attccggaac cattcttcct
      241 gcctttgttc gagggttttt acatcggtga gggccaggtc ggatgcggta aaactggctg
      301 aaggactggg ttccaggcgg gtgttgcggt cgttgagcat gctgaatttt tcggattcga
      361 catgacggcg ggattcatcc ttgcggtatt ggtcgaggtt gagcgaggct gtcgtctggt
      421 ccctgaggct ttttagccgg agtgcgtttt catcaataag gttgaatacc ttgctggtat
      481 ccgtcctgtt tttactcagg gtgcgcaact ggtccaggtt gtctatggtg aacacatttt
      541 gctgatagga gagtttgggc agttcggtcc attccatcgc atgatcgtat tccctttccc
      601 cgatctcgag gtatttatag gtatccggca gttcgatgtc cggctgcacg ccgcggagct
      661 gggtggagcc gccgttgatc cggtaaaatt tctgcacggt cactttgatt tcgcccaggg
      721 gtttataggc ctgcagaccg ttgaccacgc ggtccagggg gaaaaaccgt tgtacggtac
      781 ccttgccgaa agaggttttc gacccgacga cgaccgctct tttgtaatcc tggagggcgg
      841 cggcgagaat ctccgaagcg gaggcgctgt atgaattggt catcacgacc aaaggtccgt
      901 catagaccac ggaggcgtcg cggtcctgca tcacggtggg atttcccgat ttggatttga
      961 cctggacgac cgggccggtt tcaataaact gcccaaccat atccaccacc tcggtgaggg
     1021 accctccccc gttgaacctg aggtcgagaa tgatgccccg gactccctgg ctttgcagtt
     1081 ttttaatttc attggccaca tccctggaac agctcttgcc gttgttgtct tcaaaatcgg
     1141 aatagaaact gggcaggctg ataaatccga ttttatccgt catgcccggg atctccagga
     1201 tggcagactt ggcccagcct tcttccagga tcacttcatc gcggaccagg tgaatttctt
     1261 tggaggtgcc gtcttttttc tttacgctca cggtgacccc ggttcctttt ttgccccgga
     1321 tgaaggtgac cacttcatcg acccgcatcc cgatcacatc cttaggttcg gcgccgtcct
     1381 gggtaacccg gatgatcagg tcgtccacct ccagttcctt gtttttggct gccgggcctc
     1441 cgggtatgat gctggtgact ttgacataat ctttttcctg tcccagcctt gcgccgatgc
     1501 cttcgtactt accggacatc tgcatattga agtcctcctt gtctttgggt gaaaaataat
     1561 tggagtgcgg gtcaaaatat ccggtgatgg cattgaggta agattcaaac cagaccttgt
     1621 cgtccagctt gcccaggcgg gaaaaataat cgtcataggt gtctttcact ttttcacggg
     1681 cttccgcctg caggtcggcc agggactttt tgggggcgcc ggactggtct ttatcctgct
     1741 catccaggcg gtcggctacc ttggaggcaa tttcatattt gatcagtttt ttccagtacg
     1801 ctttgagctc ggcctcgttt ttggcatact gcattttatc cgggtccagc tcaatggtct
     1861 cattcccttt aaaatcatag ttatgtttga tcgcttcttc gtaatacccc tgggcccggg
     1921 tcagggagga cttaaaaagc cgggcggcca ggtaatgaaa ttccaggctg cggcctttca
     1981 cttcttcatc cagccggagt tcaaaaggct ttaattgacc gatttcgttt tgggtaaaaa
     2041 aacgtttgga cccgtcgagg tcctggagat ggcttttgta aaccgccttg gaaaagtcgt
     2101 cgttcagctg ctgggggcgg aaatgggctt gttccaggac ctgcatgacc gtatgcagga
     2161 tcacggcttc cttggacccg tcggtttccg gcataccctt tttaccggct gagaaaacaa
     2221 aaaacaaaac ggcaatgccc atggcaaaca aagaacctct cataatccat ttagatttta
     2281 aactcatgct aataaaccga aaacccggct gtaaggctgt taagatttaa ataaatgttt
     2341 aaaacaagag gataaatata agggaattgg caaaagagcg gcggggtcat tccccaagcc
     2401 atgtcttaaa cgccttcgcc ctttcccggc tcaccagaat ctcttcctcc gggtcgggtt
     2461 ccatttcaac cttcagtttt gaattaaacc aggggtgcac cgccttgatg gcctgccggg
     2521 agatcaaaaa ctgccggttg gccctgaaaa actcgtccgg atccacctga ttttcaattt
     2581 cttccagggt ataggccagg atatattttt gtttatcccg gctcaacaac tgggggaccc
     2641 catgacttac cataaagagg gcggtattac cagcctcgac cgaaatccat ttttgaccct
     2701 tttttaccag gaatcgttcc cggtactcgg ttttccgggg gatcatttcc cggatgagat
     2761 tcgccagctt ccccgcacct tgaccggaag catggaccgt tcgctcaaat ttccgtaggg
     2821 cgatggacaa ctcctccttg ttgatgggtt tgagcaaata atcgatgctg ttgaccttaa
     2881 aagcctgaat ggcatattcg tcataagcgg tggtgaagat gacgggggca tctaccctgt
     2941 ttttgttaaa aatttcaaac gacttgccgt cacccagttc tatatccatg aatatcaggt
     3001 cgggttcagg atgctgcttc agccagtcgg tcgtctcccg gacgctccgc gtgatgccgc
     3061 agacctcata ttccggccgg atttcctgta gcaaattctg caatcttttg ctcagcaggg
     3121 cttcgtcttc cacgatgagc actttcatgc gcgccctccc ggaattaagg gtaaatgaac
     3181 catgaaccgg tcttccgtct ccacgatttc gacgtctttc tgatgcatga gtttgtattt
     3241 ggtcatgatg tttcggagcc cgactttgtt ggacaagccg atgttgtttt tacgttgcaa
     3301 attattgctc acggtcagga tgccgtccgg tgtgacggag agttctattt ttaatggttt
     3361 ttccggcagg ataatattgt gtttgatggc attctcgacc aggggctgga gggtcagggt
     3421 cggaagttta taatccatcc aggcttcgtc aatgcgaagg tctatcgaaa ttccgtcccc
     3481 aaaccggttt ttgaaaagtt gaaaataagc ctttaggaat ttcagttctt cccttaaggt
     3541 cgcggatatt ttttcgttgg cctgcagcag gtaccggtac acggccgaca ggtcatccac
     3601 aaactgcacc gctttgttgg ggtcttccag gatcagggag gaaagggagc ccaggctgtt
     3661 gaataaaaag tgcgggttga tctggttttt cagggattcc agctgggtct gcaggttcct
     3721 tgctttaagt tcctcggcct ccacgaccgc ttctttccac ttcatgaaaa aatagagggc
     3781 ttcgtaaatg ccggtcacga tgatgacaaa cagcagggcc acaaaaatgg cttccagata
     3841 ggcctccagc ggaaagggat attcggacca aagcgcggtt ttatcataaa tccagaggtt
     3901 gaaccagcgg atgaatacgg tcacggggac cgtgccggcc agcgtccagg ctatccgttt
     3961 cggggtttgg tttaatgcag ggaaccgcct ccggaaatag aggatcacca accggatgct
     4021 ttcccagacc gcgaaggttt caagcaggga gaaaaaataa atgccccaga acccaaatcg
     4081 ctgatcgccc gattcgttcc tgttgtaaaa gatcaggtgg gccatcaggg caataaaagg
     4141 gatacccagc aaccggaacc aggtatcctt caagcccgcc tttttattca tgtcttcctg
     4201 atttcatttc aaggccggaa gtttcaattg aatttagcca catagttaca caattcattt
     4261 ttcaggtcgg tgattttaaa caaataaggg ccaaacaact gacgtcccac attggtctgg
     4321 atgtaaaggt aggtatcgtt gccggggaaa tagatgaact gggtactgtt gccaatgcca
     4381 tccccttcgt gtccgtattg aagttggccg ccgtaatggt aacattccag gcccagcccg
     4441 tattcagcca ggccggaggc tttaccggtg aaccattttt tcatttcctg aagagaagat
     4501 tcattcacca gtctcccgtt taacagggcg gtgtagaacc ggatcaccgc ttccggactt
     4561 gcggcaagcc ccccgaaagc attgctggcc ctggccaaag cgccgttcca ggtcttgcaa
     4621 ttttcaagct gttcattggc ataacggtca aaataataat ccggaaagcc gagggattcc
     4681 gtctgctcag ccgacaggcc gtaccaggca tcctgcagga tcgtgggctc caggatttcc
     4741 tctctcaata aggtggcata ggatttttgg ctgatttctt ccaggatggc atgcatcaac
     4801 aggtagttgg tgttggaata agtgaaatcg gtcccgggtt catacaacaa gggtagcccg
     4861 gcaatcatat cgaccatttg gtcgggactg ggttggtgca ggggatcatt aagttggccc
     4921 accataaatt caggcagttc cgtaacgttg accagtcctg aggaatgatt gagcagcatg
     4981 cgcagggtga ttttttcaac cgatgggacc tttggccaga tcctggaagg caggtaatct
     5041 ttaatgttgc ggtccaggtc gagatttcct ttttcgacct gcttcaggac cagcaccgcg
     5101 gtaaaggtct tggtgatgct gaattgccag gaaggggtac agggttcgaa ggccgacctg
     5161 gattccaggc gggcatatcc acgcgtggcg aaaaaccagc catccttgtt tttcagcatg
     5221 acttccgcgc cgggcaggcc tttggcgaca tatttggcga gtatggcctg gagggaatct
     5281 ttcatcggat gattggtatt gaacaccctt agattttcac aggaaccagg attgcccggt
     5341 atgtcctccc gggtacagga caaaagaata aaaactaaaa gccagatggg gaaaagaaga
     5401 taaattttca tgacagaatt tatttattga tttttaaacc gaaagaataa ctggacaaag
     5461 gcaaaaagga aaggtcgggg ctgtagccaa acacaccctg tacctggtag gccaggtaag
     5521 gactcagcct gatattctga attttgaccg accgatagcc caggcttaac tggaggggca
     5581 cctggaacaa gcccttgaat tttttgcctt tctgccagtc ctgtccatcc caggtaaggg
     5641 tggaaccagg ataacccgac agcctatacc caatgcccgt acctaaaccc aattcgaggt
     5701 gtttcgcgat caccggagta aaccgggcga ccaactgcgc aaaagacgcg tcccccatgg
     5761 atttgctccc ggacaatcca aactggaaac ccatggaaaa tacctgcctg ctgcccgtat
     5821 gatgatagac cccgaattgg gtaatcagac ccgtaggatc atatttaaga tttttaaccg
     5881 gtgcatatac ggaatgggta ccggccgcca gatcgacttg ccaggaggat gcaggggatt
     5941 gcgcccgcag ccatgagagg gtcatcgtac ccattgccag gagaatggat aacttcattt
     6001 ttttcatttc aattgatttt tatttccctg caaattggga ggaggaagga gggagtgaaa
     6061 gtggttttag ggtcaaacgg agaaatgggg gtgtgaaacg gagaaattag gagttagaag
     6121 ctgggagtta gaagctgggg gaggccttgt gcccggcatt cctgcgaatt caaagtcggt
     6181 cccgtattca attggcgtag ctgggaagcg gtttgggcga gcgctcccat cttccatctg
     6241 ccagctccca ggagttagaa gctagaagcc agggaggcct tgtgcctgga gattcctgca
     6301 aattcaaagt cgttcccgta ttcaattggc ttagctgcga agcggtttgg gcgagcgccc
     6361 tagctcctag ctcccagctt ccagctccca ggagttagaa gctaggagtt agaagtcagg
     6421 gaggccttgt gcctggggat tcctccaaat ttaaagtcga tccctgtatt caaatggcct
     6481 ggctgggaag cggtttgggc gagcgcccta gcttccagct tccagctccc agcttcctgc
     6541 ttccagcttc cagcagccag gagttagaag ctagcgaggc cttgcgcctg gggattcctg
     6601 caaattaata gtcggtccct gtgttcaatt ggcgtagctg tgatgcggtt ttggcgggcg
     6661 ccctagcttc cagcttccag ctccctgcta gtacaccggt atatacacaa tacacttccc
     6721 cctgaaatac tcctcgttga aaaactcatg aatcatgaac agttcatggg gtacactttt
     6781 tatggtcttg tgttcctctt tcagcgggta acctttgtag gcgatgaggc cattgggtcg
     6841 ggcattaata tggcgggtcc ggatgcgttt tcgggagagc cggagcaggt cggccaggct
     6901 ggtgaccgcc cgggccacca cataatcgta ttggctgctc aggtcttcga cccggatatg
     6961 actggccgtc acgttttcca atctcagatg ttgtaccgca gcctccacca cttttatttt
     7021 tttcccggta ccgtctatca ggtggaagcg gacctcgggc cagaggatgg ccagcggaat
     7081 gcctggaaaa ccacccccgg tgcccaggtc gaggatttct gtgcccggcg caaaattcat
     7141 aaaacggcct aacgtgaggc tatggaggac atggtgcagc atgaactgct ccatatcttt
     7201 tcgggagatc aggtttattt tttcattcca ttccctgtaa aagggttcca gggagtcaaa
     7261 ccgggatttc tggatctccg aaagattggg gaagtactgt tcgataagtg caggcatgat
     7321 ggtggcaagt tacatatatt ggggtgagca tgtattgcct tttatggata gaaataacat
     7381 gattttagat ttgattcgca gtgagaaagt cattattaac cgcaaaggac gctgagggcg
     7441 caaagaatac gcagaggaga tttatgattg cattttctct gcggccctct tcgcgattca
     7501 ttgtgcgctt tgcggttgag aaagtgatta ataaccgcag agggcgctga gagcgcgaag
     7561 at
//
LOCUS       DAOQXT010000165         7321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_10556_length_7321_cov_6.311726, whole genome shotgun sequence.
ACCESSION   DAOQXT010000165 DAOQXT010000000
VERSION     DAOQXT010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10556_length_7321_cov_6.311726"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKM27_18335"
     CDS             complement(<1..202)
                     /locus_tag="PKM27_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNR09285.1"
                     /translation="MPDKNEVIDDLDFKILSKLVVNADRPYTDIAKELYVSGGTIHVR
                     IKKLKKLGIIKRALLDIDYHKLG"
     gene            240..1724
                     /locus_tag="PKM27_18340"
     CDS             240..1724
                     /locus_tag="PKM27_18340"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR09286.1"
                     /translation="MPDKGNPKKYEIAQPLLLACTVVLGMLLGRKMEQSNQPEQKNIP
                     RPNTATRVHSGLIDEAVRYIDARYIDSVGTGHLTDLAIESMLENLDPFSVYLPAGTVP
                     KDSIESFEEAYGFSPEYVRNQWVIGSPDRKFGSLQNGLAAGDQIVSVSNKPPDRDIWL
                     NWKADSLHLRVRKPETREIKEVLVRRASGVPIIPRSMALALNARLAYIRIPHFGDQAY
                     DEFITAVDSFYNKHKIRHYVIDLRGNSGGYLEECSRILNQLFKENNLLLVSTKGRTVR
                     QTEYKTDGRQLFDLGQVVVLIDGGSASASEAFAGAVQDLKRGRVVGQASYGKGLVQEQ
                     YMLSNGSALRLSVARFYLPSGRTLDHEGKYEPSFEELKAVQGKKQLVHRSIIPDVRVA
                     RDTTMELPAWLNLKKLVRVLALESLLDLREGRKASEPEARIFRTLNTNSAPLVRNLVR
                     SNPSLVKTMIRQEWLAANMQFTQAEKEDLLAQKEIQTAVQLIRN"
     gene            complement(1914..2483)
                     /locus_tag="PKM27_18345"
     CDS             complement(1914..2483)
                     /locus_tag="PKM27_18345"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNR09287.1"
                     /translation="MKLSQEIENLVDCLKSGGVALYPTDTIWGLGSDMNSKAAVDRIY
                     AIKKRSKDKPLILLVSGLEMLKNYVPSIHPRLETLLSHFDKPLTVVYPKVAGIPSWLR
                     SEKGEVAIRVVRDEYCQQVIDRLGNPLTSTSANQTGQPFPKHFGEIQSDIMQQVDFIS
                     NYRREDKTAREPSVIIRCLPNGEFEFLRE"
     gene            2540..4801
                     /locus_tag="PKM27_18350"
     CDS             2540..4801
                     /locus_tag="PKM27_18350"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNR09288.1"
                     /translation="MFKEPASHLIRFLKYLLPPLAFLTASNKLLAEHLVGGTMTYRCI
                     GDGSIPNTKNYRVRLTLTRDCSQFSNFSSTIEIGLYRFNGSQYVFSRKLSGAIQPERM
                     IPQDQKSCSFLQYTVCVKEAYYEIDFQDVPVIDQTYLFAFQECCRNMTISNITEPGRT
                     GYTIMVQVTAEAQRICNQSPSSDLIPPLVLCANQEMNLDLASTDAEGDQLTYELCAPL
                     AGGGPAGLNSVGDPVSCNGIRPNPALCPPPLTAVNYSSAGTTAQNPFPSAIPITLING
                     ILRALPNMVGQYVFGICIKEYRNGILLSVQQREMQINIDVCSSPTASIQADRTSNDTA
                     YIRICDPDEEFRIINTTADTQLVKSISWEFTPPSGANWSDSTFDFTRTLADTGLYTGL
                     MILNKGNQCSDSIHIRLKVNPKLEVRPDAVYDSCRFDPIQFGVQVISGQPVSFEWEFG
                     DSTRSDLRDPVHAYASPGEYMARLTTQDAQGCSDVQFIHLVLFPAPPDFDLSIPDSII
                     CYPDSILLGIAGISDPSYQYRWEVSNGIILTGPSPKLGLNVGGRFSIKATLISKTGCR
                     SEKTFSILSLESPVAEFDLVSDQLNLRQPDLMVINRSRNASSYRWDFGDGQFSTQVNP
                     SYAYQGAGSYTVQLVAYHVNGCSDTVQRPLSVSAASDFYLPNIFTPNGDGQNDIFKPE
                     GIEGFFSGYDLSIFDRWGSLLFFSEDQSQGWDGTRGATNKMALDGVYIYVVRFATEDG
                     EKKIVKGSVTLIR"
     gene            4798..7095
                     /locus_tag="PKM27_18355"
     CDS             4798..7095
                     /locus_tag="PKM27_18355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNR09289.1"
                     /translation="MRKFAPVFALAALLMLLGGRVQARHIIGSDFYYVCLGEGRSSGT
                     KRYGFHLDVYRDCSTNILFNPNAAFGIYKFSESRGYTFVNQFNVPHGTISNVKPDQNP
                     CIIIPPNVCVETTDYEFSIDLPTIDETYVIFYIQCCRNRTILNIPDPGSTGATFFIEI
                     SPTAQRTCNNSPRFNTFPPIVVCADFPLDFDHSARDDEGDSLVYEFCPLLAGGGAGGG
                     GPFGGGGFPGGGLGGCTTPTPDPRICPPPFNTLRSVSGYSYEAPMGPGVLNLNAETGR
                     LTGRPVNLGQYVVGVCVKEYRNGVLIGSIHRDFQFNVAICEQSVYAKIKADSTSLIDK
                     KFKITYCGDNLIKFKNESYTEEFIKDYYWEFRPKNNPTSSERLTSLDKDASIAFTRPG
                     QYTGVMIVNRNALICNDTAFIELNIIPSDIKADFEFEYDKCSTLPIKFTDRSGGVITP
                     VKTYEWNFDDGVRSAARSPSHLFQKPGNYAIKLQVTDGKLCRSEMVKTLNYFPAPQLL
                     DILPDKFRACVPATINFRNLSIPVDETYQVEWDFGDGNKFTGIHASHTYTRPGTYTIG
                     LSLKAPSGCVTKETFPGFVRVQEGPEADFRFSPDDPNLLNPTVQFNNLSRDAVEYAWD
                     FGDESGGTEKNPIHSYSDTGNYLVNLSVRHENGCRDSAVRLVRVGLYITYFLPNAFTP
                     NNDGVNDLYFGTGSLPGMQDFNLSIFNRWGELIFTTEDPTEGWNGRKQNKGPMEPNGV
                     YICIVKYKNDRGDPREVSGFATLIR"
     gene            7130..>7321
                     /locus_tag="PKM27_18360"
     CDS             7130..>7321
                     /locus_tag="PKM27_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09290.1"
                     /translation="MTGLKDFRNLFKKLIFFTLVASSGITAYARHIIGSDFYYDCNGP
                     GRGGNTKNFSFNLTIYRDCS"
BASE COUNT         1827 a         1967 c         1782 g         1745 t
ORIGIN      
        1 atcccagttt gtggtaatca atatccagca gggcccgctt gatgatgccc agctttttaa
       61 gtttttttat ccgcacatgg atggtcccgc ccgaaacata caattccttg gcgatatccg
      121 tgtaaggccg gtcggcattc accaccagtt tggacagaat cttaaaatca aggtcgtcta
      181 tgacctcatt cttatccggc atcgaccaat aactttttaa ataatagctt tgcagaaata
      241 tgcctgataa aggtaatcca aaaaaatatg aaattgccca gcccctcctg ctggcctgca
      301 cggtggtcct gggtatgttg ctcgggcgga aaatggaaca aagcaatcag cccgaacaaa
      361 agaatattcc aagacccaat acggctaccc gggtacactc cggcctgatc gatgaggccg
      421 tccggtatat cgatgcccgg tatattgatt ccgtgggcac gggccacctg accgacctgg
      481 ccattgaatc catgctggaa aacctggatc ctttttcggt ctacctgccg gcaggcacgg
      541 tcccgaaaga ttccatcgaa tcctttgaag aagcttatgg cttcagcccc gaatacgtca
      601 gaaaccaatg ggtcatcggc agtcccgacc gaaagttcgg atccctgcaa aacgggctgg
      661 ccgccggcga ccagatcgtg tccgtcagca acaaaccccc ggaccgggat atatggttaa
      721 attggaaagc ggactccctc cacctccggg tacgtaaacc ggagacccgg gaaataaaag
      781 aggtcctggt ccgtcgcgca tccggagtgc ctataatccc gcgatcgatg gccctggctt
      841 tgaatgcccg gctggcctat atccgtattc cgcattttgg cgaccaggcc tatgatgaat
      901 tcatcacggc ggtggacagt ttttacaata agcacaagat ccggcattat gtgatcgacc
      961 tcaggggaaa ttcgggagga tatttggaag aatgttccag gatcctcaac cagcttttta
     1021 aggagaataa tctcttgctg gtgagcacca agggcaggac cgtacgacaa acggaatata
     1081 aaacggacgg gagacaattg tttgacctcg ggcaggtcgt agtccttatt gacggcggtt
     1141 cggcttcggc cagcgaggcc tttgccggag ctgtccagga cctgaaacgg ggccgggtgg
     1201 tcggccaggc ctcttatgga aaaggactgg tccaggaaca atacatgttg tccaatggct
     1261 cggcccttcg gctttccgtc gcccgctttt atctgccttc cggccgcacc ctggaccatg
     1321 aaggaaaata tgaacccagt tttgaagaat taaaagcagt ccaggggaaa aaacaattgg
     1381 tccaccgctc gatcatcccc gacgtacgcg ttgccaggga tactacgatg gaattaccgg
     1441 cctggcttaa cctgaaaaaa ctggttcggg tgctggccct ggagagcctc cttgacctcc
     1501 gggaaggcag gaaggcgagc gaaccggagg ccaggatttt tcgtaccctg aacaccaatt
     1561 ccgcacccct tgtccgcaac ctggtcaggt caaatcccag cctggtcaaa acgatgatcc
     1621 gccaggagtg gttggcggcc aatatgcagt ttacccaggc ggaaaaagaa gacctcctgg
     1681 ctcagaaaga aatccagacg gcggtgcagc tgatcagaaa ttaagggcac ctctaaatac
     1741 cctgaatgag tttgaaaatt agctattttg aatttatact aggcgaatcg aaggagcata
     1801 gcgagctctc tgtgactgag attcaacgca gtataaattc aaaagagcaa tttaggtttt
     1861 tgatataata aaccgatcaa accatgaagg gtgtttagaa gtgcccttaa ggatcattcc
     1921 cgtaaaaatt caaactctcc attggggaga caccggatga tgacggacgg ctcccgggcg
     1981 gttttatctt ccctgcggta attcgatata aaatcaactt gttgcatgat atcggactgg
     2041 atttctccga aatgttttgg gaagggttga ccggtctggt ttgcagaggt agaggttagg
     2101 ggattaccca gccggtcaat gacctgctgg cagtattcat ccctcaccac ccgaatggcc
     2161 acttctccct tctccgagcg gagccaggag ggaatccccg ctaccttggg gtaaactaca
     2221 gtaagcggtt tgtcaaaatg actcagcagg gtttccagcc tgggatggat ggaaggaaca
     2281 taatttttta acatttccag tccggatacc aacaggatca ggggtttgtc tttggaccgt
     2341 ttttttatag cataaattcg gtccaccgcg gccttggaat tcatatcaga acccagtccc
     2401 cagatggtat cggtaggata cagggctacc cccccgcttt tcagacaatc caccagattt
     2461 tcaatttcct gagatagttt cattcgggct gcaaaccaaa caaaaaataa taaattaaac
     2521 taatttgcag ttgttattat tgtttaaaga accagcaagc catttgatcc ggtttttaaa
     2581 gtatttgttg ccccccctgg cctttttaac cgccagcaat aaactgcttg cggagcattt
     2641 ggtaggcggt acgatgacct accggtgtat aggcgacggt tcaataccta ataccaaaaa
     2701 ctaccgcgtt cggctgaccc tgaccaggga ctgcagccag ttttccaatt tcagttccac
     2761 tattgaaatc ggcttgtatc gttttaatgg ttcacaatat gttttctcgc ggaaactctc
     2821 cggggcgatc caacccgaac gcatgattcc ccaggaccag aaatcctgca gttttttgca
     2881 gtataccgtc tgcgtgaaag aagcttatta cgaaatagac tttcaggatg tgcccgtcat
     2941 cgatcaaacc tacctctttg ccttccagga atgttgccgc aatatgacga tcagcaacat
     3001 cacggaaccg ggaaggaccg gctataccat catggtccag gtgaccgcgg aagcgcagcg
     3061 gatctgcaac cagagccctt cctctgacct gatccctcct cttgtgctct gtgccaacca
     3121 ggaaatgaac ctggacctcg cctccacgga cgcagaaggg gaccaactga cttatgaact
     3181 ttgcgcgccc ctcgccggag gcggacccgc ggggctgaac agcgtcggcg atcctgtcag
     3241 ttgcaacggc atacgcccga atccggccct ctgccctccc ccgctgaccg ccgtcaatta
     3301 cagcagtgcc gggaccactg cccagaaccc ttttccttct gcgatcccga tcactttaat
     3361 caacggaata cttcgtgcct tgcccaatat ggtcggccag tatgttttcg gcatctgcat
     3421 caaggaatac cgaaacggga tcctcctgag cgtacaacaa agggaaatgc agatcaatat
     3481 cgatgtatgc agttctccga ccgcctccat tcaggccgac cggacttcca acgacacggc
     3541 ttatatcaga atatgcgacc cggatgagga attccggatc atcaatacga cggcggatac
     3601 ccaattggtc aaatccatct cctgggaatt cactccccct tccggtgcaa actggtcgga
     3661 cagcactttt gattttaccc gaacgctcgc cgataccggg ctctataccg gtcttatgat
     3721 cctgaacaag ggcaaccaat gttcggattc catccatatc cgattgaagg tgaatccgaa
     3781 attggaggta aggccggatg cagtgtatga ttcatgcagg tttgacccga ttcaattcgg
     3841 cgttcaggtg atcagcgggc aaccggtatc cttcgaatgg gagttcggag actctacgcg
     3901 gtcagacctc cgggacccgg tgcacgccta cgcctctccg ggagagtata tggcccggtt
     3961 aactactcaa gacgcccagg gatgttcaga cgtccaattt atacacctcg tcttgttccc
     4021 agcacctcct gattttgact tatccatacc ggattccatc atatgttatc ccgacagcat
     4081 tctccttggc atagcaggca tttctgaccc ttcctatcaa tatcgctggg aagtaagtaa
     4141 cgggatcatt cttacgggac catctccaaa acttggcctg aatgtgggcg gccgcttcag
     4201 cataaaggcc acgcttattt caaaaacagg gtgccgcagt gaaaaaacct tttcaatcct
     4261 cagtcttgaa agcccggtcg cggaatttga cctggtttcc gaccaactga acctgcgcca
     4321 gccggacctg atggtcatca atcgttccag gaatgcttct tcctatcgat gggactttgg
     4381 cgacgggcag ttttctaccc aggtaaaccc ttcctatgca taccagggag cgggcagtta
     4441 tacggttcaa ctggtcgcct accatgtaaa tggatgcagc gatacagtgc aaagaccgct
     4501 tagcgtttcg gccgcttcgg atttttacct gcccaatatt tttacaccga acggggacgg
     4561 ccaaaacgac atcttcaagc cggaaggcat cgaaggtttt ttctccggtt atgatttgtc
     4621 cattttcgat cgctggggct ccctcctgtt cttctcggag gaccagtctc aaggctggga
     4681 cggcacccgg ggagccacaa acaaaatggc cctcgacggc gtttatattt acgtggtccg
     4741 gtttgcgacc gaagacggtg aaaagaaaat tgtaaaaggc tctgtaactt taattcgatg
     4801 aggaaatttg ccccggtatt tgcgctcgca gccctgctta tgcttttggg cggcagggta
     4861 caggcccggc acatcatcgg aagcgatttt tattacgtat gcctgggtga gggccggagt
     4921 tccgggacca agagatatgg atttcacctg gacgtgtacc gggattgcag caccaatatc
     4981 ctgttcaatc caaacgcggc tttcggcatc tataaattca gcgaaagcag ggggtacacc
     5041 tttgtcaatc aattcaatgt accccacggc acgatttcaa acgtaaaacc ggaccagaac
     5101 ccctgcatca tcatcccgcc caatgtctgt gtggaaacca ccgattacga attttcaatt
     5161 gatctcccga ccattgacga aacttatgtc attttttata tccagtgctg cagaaaccgg
     5221 accatcctga atattcccga tccgggatcc accggggcca cttttttcat cgaaatcagc
     5281 ccgaccgctc agcggacctg caacaacagt ccgcggttta ataccttccc gcccatcgtc
     5341 gtctgtgccg actttccgct cgattttgat cacagcgcca gggatgacga aggggattcc
     5401 ctggtttatg aattttgccc cctgctggcc ggtggaggcg cgggcggcgg aggtcctttc
     5461 ggaggcggag gttttccggg tggcggtctg ggcggctgca ccactcccac cccggatccc
     5521 cggatctgcc cccctccctt caataccctc cggtccgtat ccggatattc ctatgaggct
     5581 cccatgggtc cgggggtgct caatctgaat gcggaaacag gcaggttgac cggaaggccc
     5641 gtcaatctcg gacaatatgt ggtgggggtc tgtgtgaaag aataccggaa cggggtcctg
     5701 attggctcca tacaccggga tttccagttt aatgtcgcca tctgcgaaca aagcgtttat
     5761 gccaaaatca aggccgattc gaccagcctc attgataaaa aattcaaaat cacctattgc
     5821 ggtgacaacc tgattaagtt taaaaatgaa agttatacgg aagaatttat caaggactat
     5881 tattgggaat tccggccaaa aaacaatccc acttcctctg aacggctgac cagcctggac
     5941 aaggatgctt ccattgcatt cacccggccg gggcagtata ccggagtaat gatcgtaaac
     6001 cgcaacgcac tgatttgcaa cgataccgct ttcatcgaac tcaatatcat tccgtccgat
     6061 attaaagccg attttgaatt tgaatacgat aagtgttcca ccctgcccat aaagttcacc
     6121 gaccgatccg gcggagtcat caccccggtc aaaacatatg aatggaactt tgacgatggg
     6181 gtccgctctg ctgcccgcag cccaagccat ctgttccaga aaccgggaaa ttatgcgatc
     6241 aaacttcagg tcacggacgg caaactctgc cggtccgaaa tggtcaaaac actcaattat
     6301 ttccccgcgc cccagttatt ggatatcctt ccggataaat tcagggcatg cgttccggct
     6361 acgatcaact tcaggaacct ttccatcccg gtcgatgaaa cctaccaggt ggaatgggat
     6421 ttcggggacg gcaacaagtt taccggcatc catgccagcc atacctatac ccgcccggga
     6481 acctatacga tcggcctttc cctgaaagcc ccgagtggct gcgttacgaa ggaaaccttc
     6541 ccaggctttg tccgggtcca ggaagggccg gaagccgatt tcaggttttc ccctgacgat
     6601 cccaacctgc tcaacccaac ggtgcagttt aacaacctca gccgtgacgc ggtggaatat
     6661 gcctgggatt ttggcgacga atccggaggt actgaaaaaa atcccattca ttcctattcc
     6721 gataccggaa attacctggt gaacctttcc gtcaggcatg aaaacgggtg cagggattcc
     6781 gcggtccgtt tggtccgggt cggtctctac atcacctatt ttctccccaa tgcgtttacc
     6841 ccaaacaacg atggggtcaa cgacctctat ttcgggaccg gcagtttacc gggcatgcag
     6901 gatttcaatc tgagcatttt caaccgctgg ggagaactca tatttactac ggaagacccc
     6961 accgaaggct ggaacggaag aaaacaaaat aaaggcccca tggagcccaa cggcgtatac
     7021 atttgtatcg tcaaatataa gaacgaccgg ggcgacccca gggaagtctc cggatttgct
     7081 accttgattc gataaaggaa cctgtttatt tgaacatttg ggctctgaca tgactggctt
     7141 gaaggatttt agaaacctat ttaaaaaact gatttttttc accttggtcg cctctagcgg
     7201 gatcacggcc tatgcccggc atattatcgg cagtgacttt tattacgatt gcaatgggcc
     7261 tggcaggggg ggcaacacca aaaatttcag ttttaacctg accatttacc gggactgcag
     7321 c
//
LOCUS       DAOQXT010000166         7245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_10695_length_7245_cov_6.032962, whole genome shotgun sequence.
ACCESSION   DAOQXT010000166 DAOQXT010000000
VERSION     DAOQXT010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10695_length_7245_cov_6.032962"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..1482)
                     /locus_tag="PKM27_18365"
     CDS             complement(280..1482)
                     /locus_tag="PKM27_18365"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNR09291.1"
                     /translation="MQPFGQVPDQRIHALDVFRGMTIFLMVFVNEVAGIKNLPGQFYH
                     LPAEADGMTMVDWVFAGFLFIVGMSIPFSINQRIRKGDTLFQLFSHILIRTLGLLILG
                     VFMVNASTGHHEASMALPIALWSLLIYPAAILIWNQYRGVPATRAWILRSLGILLLIA
                     LALLYRGGQDGSKGLSPQWWGILGLIGWAYLFSSLLYLLSRGRLLVLIAGLAFCTLIY
                     TVGHTELSNQLAFLSWTRDSGGHATHTCIALCGMVLSLILFDTGNKWLGSRRVYPALG
                     WAVLLLIAGMLLRPYFHIGKIPATPSWGFYSSFFSVLAFLLIKWISDLKKSTSWASFF
                     EPAGSNPLLTYIIPFILWAFYRLFDFYPLPDEYRTGLTGVLFCILYSSAILWLVRILN
                     RMNIRLQL"
     gene            complement(1482..2606)
                     /locus_tag="PKM27_18370"
     CDS             complement(1482..2606)
                     /locus_tag="PKM27_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HNR09292.1"
                     /translation="MKPLLPLLVCLGGLMLNSCSTKAPAPPQKAIIGYVPGFRGELKD
                     LNIDATKMTHINYAFVDVRDSMAWLTNMETDTVNFKYLVSLREKNPQLKILISIGGWS
                     WSENFSDAVLTPSSRKKFAATSVDIIHRHGLDGVDIDWEYPGFKGEDNVFRPEDKENF
                     TLMFQAIREELNRLTAQTGKPYLLTTAIPCFQEFIDRTEMGKLQEYLDFVNLMSYDFY
                     VAGDTAGHHTNLYPSTSYEYNESAQKAFEAYTAAGVPASKLVMGIAFYGRSWIMKSND
                     NWGIDRPVDSVARGGGYSYIKDSLSVRPGFVRHWDDKAKAPVLFNETTRQMVSYDDEE
                     SVMHKCNYVKEHQMGGVMFWQYASDPKLYLLDAINQNLRP"
     gene            complement(2652..5060)
                     /locus_tag="PKM27_18375"
     CDS             complement(2652..5060)
                     /locus_tag="PKM27_18375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNR09293.1"
                     /translation="MKKSYLVLWLLCTAYFSGAQKLDHVQGEILVQVQKESDIATILS
                     RYTALRRSGTVRALAPQARIYSLRFDFAKYHEGELLEKVRKHPKTLAAQFNHLITPRQ
                     NIPDDPSFSLQWQWLNNGVNGKTKGIDTKAYLAWNKSTGGMTRQGREIVVAVIDDGTE
                     LTHPDLMPNTWVNEDEIPSNQLDDDQNGYVDDYLGWNILSGNDSIGEGEHGVSVNGLI
                     GAAGNNQTGVSGINWNVKMMNLKYDVRQGIKESEVISGYLYVLNQRKIYNASQGQKGS
                     FVVATNASWGIDNGKPEDAPLWCGIYDSLGKVGILNVAATDNRTQVNIDSLGDLPCLC
                     PSDFLLAVTAIGSDGKRTGAYGVNNIDLAAPGDNVYTTRPMGMYGFDSGTSFAAPVIA
                     GAIGYLYSNSCNQLESLALTDPSAATLQVREAILSSVEKLESLAGLVHSGGTLNLLRA
                     LDRIQIACTACTSRGLESRIYIDSLILDGMKFRSRDNFGYGDFTAVDSLRPVINLDGQ
                     ILLKVYPYLKDSLTQYYLRVWLDRNHDHDFSDTAELVWDSGPTRLTGNLVSNIHLSPT
                     LINSTGISTLRISLKAAVNIGDTALPEPCDFFNAGEVEDYAIQIRPKSFDCPDVLELD
                     STAVYETSAIIRYQQILPKLFYLVRYREASSAKWDTLPTRDTMVRLDKLKECTEYIVE
                     SKTVCDSDTTRFKNMFRFRTLGCTVPVREISPFSQVNIFPNPFSTRFTVRFSTERPLE
                     NVVFRVVDIRGSVLQTRALGRLGSGTHDIAGENYSGLSMPSGVYFVQLLSQEGAISRK
                     ILKF"
     gene            complement(5078..5659)
                     /locus_tag="PKM27_18380"
     CDS             complement(5078..5659)
                     /locus_tag="PKM27_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNR09294.1"
                     /translation="MLGLKLATDPRWVNLAEKSLEEILTDHAYCEQKAASSCISLIQQ
                     YPDKKDMVEALAPVVTEEWGHFRMVLRKLDEKNLKLGYQRNDEYVNALLRFQAKGGSR
                     ESRLVDRLLICAMIEARSCERFRLLSLHLAEQELRDFYHTFMVSEAGHYRLFIDLAKQ
                     YGNPEKVQTRWQELLDYEASVLEGFALRGDRMH"
     gene            complement(5659..6762)
                     /locus_tag="PKM27_18385"
     CDS             complement(5659..6762)
                     /locus_tag="PKM27_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09295.1"
                     /translation="MSSLNRFIGTGMVLLASLIWPYQPDAQVTAKGLWGPHPQEFVQS
                     MATQASLDREKIQQALRSEKQNHDQLQEQLKVSKGAAASSLRTDIKNSSNKLEQLQEE
                     LAAIIKRGKEFKDLIGQPDKTVIRKLEKMDPDVFASRSKNPEQKKAEPTKPVAVIAPP
                     DKITNKTSQAETRPTEEPTVQPKGNLPASSPAFPNALPLPSSSVQQDCLFQSAGSDNK
                     PGLALRPEILLKYTPDVLRKYMGGQAYITGRAFIATEPGITYLQLHLEFATEQALRQY
                     GNLGKSILLIRLINGKEVRLVANRFDQGMADPELKKSTMSGIYYVDKPGERLLADTPV
                     DRIRLIFATGYEDFVIYNVDFFTRQLACINAQK"
BASE COUNT         1738 a         1777 c         1980 g         1750 t
ORIGIN      
        1 gggtagcata aaatttaggg atttaacatc gtcccggatg ggacgaaata acctacctga
       61 tttgggttac ccacttgagg tgcctaccgg cactattaac cgtaaaatgc cggattgtga
      121 ttttcccaat acagtatgtt gttgcgactg ccgggccgta gtccgaaaaa ggggatattt
      181 aaatttccaa ttacgaagga ggcttctgat acccggattt ttttctcatg ggccgaagca
      241 aagcttcgtc cgaacctggc ttcgtccgaa caaagggatt tacaactgca atctaatatt
      301 catccggttc aaaatccgga cgagccaaag aatggccgaa gaatacaaaa tgcaaaaaag
      361 cacaccagtc agccctgtcc tgtattcgtc gggcagagga taaaaatcaa agaggcggta
      421 gaatgcccag aggataaaag gaataatata agtcagcaac ggattggagc cggcaggttc
      481 gaaaaaggag gcccaggagg tggacttctt caggtcgctg atccatttaa tcaataaaaa
      541 ggccaggacg ctgaaaaacg aggaataaaa gccccaggaa ggagtcgccg gaattttgcc
      601 gatatggaaa tatggccgca gcagcatacc tgcaatcagg aggaggaccg cccagccgag
      661 ggcaggatat acccggcgac tgcccagcca cttattgccg gtatcgaaga ggatgagtga
      721 caggaccatg ccgcataagg ctatgcaggt atgggtggca tggccgcctg aatcgcgggt
      781 ccaggataaa aaggcaagct ggttggataa ttcagtatgc ccgaccgtat aaatcagggt
      841 gcaaaacgcc aggccggcta tcagaaccaa aagcctgccc cggctgagga gatagagcag
      901 gctggaaaac aggtatgccc agccgatgag tccaaggatc ccccaccatt gagggcttaa
      961 tcccttactg ccgtcttgac cgccccggta aagcaaagcg agggcaatca gaagcaaaat
     1021 gcccaggctg cgcaggatcc aggcccgggt ggccggcacc ccgcggtact ggttccagat
     1081 caggatggca gccgggtaga tcagcagcga ccagagtgcg atggggaggg ccatggacgc
     1141 ttcgtggtgg ccggtggagg cgttcaccat aaaaaccccc aggatcaaca agcccagggt
     1201 gcgaatgagt atatggctga agagctgaaa aagcgtgtct cctttccgga ttctttgatt
     1261 aatagaaaag gggatggaca tgcctacgat gaataagaag ccggcaaata cccagtccac
     1321 catggtcatg ccatccgcct ccgcggggag atgataaaac tggccgggca ggttttttat
     1381 gccggccact tcattgacaa aaaccatcaa gaagatggtc ataccccgga atacatccag
     1441 agcatggatc cgctggtccg gaacctggcc aaatggctgc atcaggggcg gaggttttgg
     1501 ttgatggcat ccaggaggta gagtttgggg tcgctggcat attgccaaaa catgacccct
     1561 cccatctggt gttctttcac ataattgcat ttatgcatca ccgattcctc atcgtcataa
     1621 gacaccatct ggcgggtggt ttcattgaat aaaacagggg ctttggcttt atcgtcccaa
     1681 tgccttacaa agcccggcct caccgaaagg ctgtctttga tataactgta tcccccgccc
     1741 cgggctactg aatcgaccgg acggtcaatg ccccagttgt cattgctttt catgatccag
     1801 ctcctgccgt aaaaggcaat gcccatgacc agtttggacg cgggcactcc cgcggcggta
     1861 taggcttcaa aagccttttg ggcggactca ttatactcat aggatgtgga aggatacagg
     1921 ttggtatgat gacccgccgt atccccggcg acgtaaaaat cataggacat caggttgaca
     1981 aaatccaggt attcctgcag tttgcccatt tcggtccggt ctataaattc ctggaaacag
     2041 gggatcgccg tcgtcagcaa atatggtttc ccggtctggg cggtcaggcg gttcagttcc
     2101 tcccggatgg cctggaacat cagggtgaaa ttttccttgt cttcaggccg gaatacgttg
     2161 tcctcccctt tgaaacccgg atattcccag tcgatgtcga ctccatcgag gccgtggcgg
     2221 tggatgatgt cgaccgaagt ggccgcgaat tttttcctgg atgacggggt gaggaccgca
     2281 tcggaaaaat tttcactcca ggaccagccc ccgatggaga tcaggatttt gagctgggga
     2341 tttttttccc tgagcgaaac caggtatttg aaattaacgg tatcggtctc catattggtc
     2401 agccaggcca tggaatcccg gacatcgaca aaagcatagt tgatatgcgt cattttggtc
     2461 gcatcgatat tcaggtcctt caactcgccc cggaagccgg gtacatatcc tatgatggct
     2521 ttttggggcg gggcaggggc tttggtggag caggagttga gcatgagacc acccaagcaa
     2581 accaaaaggg gaaggagagg tttcaaggaa ttaaagaatt aaagaattaa gaattaaaga
     2641 gaagggcttg atcagaactt caggatcttt ctgctgatcg cgccttcctg gctgagcaat
     2701 tgcacaaaat aaactcctga gggcatggaa aggccggaat aattttcacc ggctatatcg
     2761 tgcgtgccgg accccagcct gcccagcgcc cgggtttgca ggacggaccc ccggatgtcc
     2821 accacccgga agaccacatt ctccaggggt cgttcggtgg aaaaccggac ggtgaatcgg
     2881 gtactgaaag gattggggaa tatgttcacc tggctgaaag ggctgatctc ccggacaggt
     2941 acggtgcatc ccagggtcct gaaacgaaac atgtttttga acctcgtcgt atcggaatca
     3001 catacggttt ttgattctac aatatactcg gtacattctt tgagcttgtc caggcgcacc
     3061 atggtatccc tcgtgggcaa ggtatcccat ttagcggagg aggcttcgcg ataccggacc
     3121 aggtaaaaca atttaggaag gatctgctgg taccggatga tggcggaggt ctcatacacc
     3181 gccgtggaat cgagttcgag cacatccggg caatcaaagg atttgggccg gatctggatg
     3241 gcatagtctt ccacttctcc cgcgttgaaa aaatcacagg gttcaggaag tgccgtatcc
     3301 cctatgttta cggcggcttt gaggctgatc cgcagggtgg atatgccggt gctgttgatg
     3361 agggtgggcg agaggtggat attggacacc aggttgccgg tgaggcgtgt tggcccgctg
     3421 tcccagacga gttcggcggt gtctgaaaaa tcatggtcgt gattgcggtc aagccatacc
     3481 cgcagataat attgggtcag gctgtccttg agataaggat agactttgag caggatctgt
     3541 ccatcgagat ttatgacggg ccgcaggctg tccacggcgg taaaatcgcc gtatccgaaa
     3601 ttgtcccggc tcctgaattt cataccatcc aggataaggg aatcgatata gatcctgctt
     3661 tccagacccc ggctggtgca tgccgtgcag gcgatctgta tccggtccag ggcccgcaaa
     3721 aggttaaggg ttcccccgct atgcacgagt ccggccagcg attcaagttt ttccacggac
     3781 gataagatgg cttccctgac ctggagggtg gcggcggaag gatccgtgag agccaggctt
     3841 tccagttggt tacaggaatt gctgtacagg taaccgatcg caccggcgat caccggagcg
     3901 gcaaacgaag tgcctgaatc aaagccgtac atgcccatgg gccgggtcgt gtacacatta
     3961 tctcccggtg cggccagatc aatattattt actccgtacg cgccggtcct tttgccatcg
     4021 gagccaatgg ccgtgacggc caggagaaaa tcactcgggc agaggcaggg caaatcaccc
     4081 aggctgtcaa tattcacctg ggtccggttg tccgtggcgg ccacgtttaa aatgcccact
     4141 ttgcccaggg aatcatagat gccgcaccag aggggtgcat cttccggttt gccgttgtcg
     4201 attccccagg aagcattggt ggctaccacg aaactacctt tctgaccctg gctggcattg
     4261 taaatttttc gctggttcag cacatacaaa tacccggaga tcacctcgga ctctttgatg
     4321 ccctggcgta catcgtattt caggttcatc attttgacat tccagttgat cccgctgacc
     4381 ccggtctggt tgttcccggc agcgccgatc aggccgttga cggatactcc gtgctccccc
     4441 tcgccgatgc tgtcattgcc ggaaagaatg ttccacccca aataatcatc cacataaccg
     4501 ttctggtcgt cgtccagctg gttggagggg atttcatctt cattgaccca ggtattgggc
     4561 atcagatcgg gatgggtcag ttccgtaccg tcatcgatga cggcgaccac tatttctctg
     4621 ccctgccggg tcatgcctcc cgtggacttg ttccaggcga gataggcttt ggtatcgatg
     4681 cctttggttt taccatttac tccattgtta agccattgcc attgcaaaga gaaagaggga
     4741 tcatcgggta tgttttgcct gggtgtgatc aggtggttga attgtgcggc aagggttttg
     4801 ggatgttttc tcactttttc cagcaattcc ccttcgtggt acttggcaaa atcaaaacgg
     4861 agactgtaaa tccgggcctg gggtgccaga gccctgaccg tcccggacct gcggagggcg
     4921 gtatagcggg agaggatggt ggcgatatct gattctttct gcacctgcac caggatctcg
     4981 ccctggacat ggtccagttt ctgggcaccg gagaaataag cggtgcaaag caaccataaa
     5041 accagatacg attttttcat agatattctt ttgaagatta atgcatccgg tctccccgga
     5101 gggcaaaacc ctctaaaacg gaagcttcgt aatccaataa ttcctgccat cgtgtttgta
     5161 ctttttccgg gttcccgtac tgctttgcaa gatctataaa aagtcggtaa tgtccggctt
     5221 cggacaccat gaaagtatga taaaaatccc gtaattcctg ttcggccagg tgcagcgaga
     5281 gcaggcggaa cctttcacaa ctgcgcgctt cgatcatggc gcaaatcagc aggcggtcga
     5341 caagccggga ttccctgctg cccccctttg cctgaaaacg aagtaaggcg ttaacatatt
     5401 cgtcgtttct ctggtacccg agctttaagt ttttttcatc cagtttgcgc aggaccattc
     5461 ggaaatgacc ccattcttcg gtcaccacgg gagccagggc ctccaccatg tctttcttat
     5521 ccgggtattg ctggatgagg gagatgcagg aggaggctgc cttttgctca cagtaggcgt
     5581 gatcggtcag aatttcttcg aggctttttt ccgccaggtt tacccatcgg gggtcagtgg
     5641 ccagtttcag tcccagcatt atttttgagc attaatgcag gcgagttgcc gggtgaaaaa
     5701 atccacgtta tagatcacaa agtcctcata cccggtggca aagatcaacc ggatccggtc
     5761 gaccggggta tctgcgagca atcgttctcc aggcttatct acgtaataaa ttcccgacat
     5821 cgtacttttt tttaattccg ggtcagccat tccctggtca aacctgttgg cgaccagccg
     5881 gacttccttc ccattaatca atctgatcag caaaatgctc ttccccaggt tgccatattg
     5941 ccgcaaagcc tgctccgtgg caaactccag gtgcagttgc aaataggtga taccgggttc
     6001 cgtggcgatg aatgcccggc ccgtgatata agcctgtccc cccatgtatt tccggagaac
     6061 atccggggtg tattttaaaa gaatttccgg ccggagtgca agcccgggct tgttgtcgct
     6121 gcccgcagac tggaacagac aatcctgctg gacagaggat gagggcagag gtaaagcgtt
     6181 tggaaatgca gggcttgaag caggcaggtt tcctttgggc tgcacggtcg gttcctctgt
     6241 gggcctggtt tctgcctgac tggttttatt ggttatttta tccggtggcg ctataacggc
     6301 aacgggtttg gtaggttccg cttttttttg ttccgggttt ttggatcgcg aggcaaaaac
     6361 gtcaggatcc attttttcca gtttacgaat caccgtttta tccggctgac cgatcaggtc
     6421 tttgaattcc tttcctcttt ttataatagc agcgagttct tcctgcagtt gttccagttt
     6481 atttgaactg tttttaatat ctgtccggag agagctggct gccgcgcctt tggagacttt
     6541 caattgctcc tggagttgat catggttttg tttttctgac cgcagggctt gttggatctt
     6601 ttcgcgatca agggatgcct gtgtggccat agactggaca aattcctggg ggtgaggtcc
     6661 ccagagccct ttggctgtaa cctgagcgtc gggctggtac ggccagatca gggaagccag
     6721 gaggaccata ccggttccga tgaaacgatt caaactgctc atgaatgcaa aatggagata
     6781 aaattacatc ccgggcttga attccgtggg aatccgcctc atttgaactg taaaagcatt
     6841 gattttaact taatgaaaaa ccggctggac gaatgccggt ctttccacgg gtgctttcga
     6901 tccagtgcct gatggagcag atttacatta gtgaataaac gagggcttcc aggtcttgtt
     6961 atatggctaa accagccata gggaaccaag gatcctggcg gtcagaggtc agaggtaaga
     7021 ggatggaggt aagaggtaag aggtcagagg tcagaggtta gagctgggag gtatgaggta
     7081 gggtagatta acctaaaata tcttcaacgc tcctgactcc aatgggggaa ccaaggatcc
     7141 tggcagtata ttcaatcaaa acagtcgtcc ttgctcttgg gaaggagtac agaaatgggt
     7201 cgaaaaagga cttcgtataa aatagtatca acacccctga ccccc
//
LOCUS       DAOQXT010000167         7197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_10788_length_7197_cov_5.468636, whole genome shotgun sequence.
ACCESSION   DAOQXT010000167 DAOQXT010000000
VERSION     DAOQXT010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10788_length_7197_cov_5.468636"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKM27_18390"
     CDS             complement(<1..380)
                     /locus_tag="PKM27_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09296.1"
                     /translation="MVLIEDEIFEAYKLDRDPQPVRFGNGLINDTYRVRINGREGLLQ
                     RINHQVFKDVPALMNNLKKVSQHVAQHFPSEIFVSLYKTREGADYMLSGGNYWRVLKF
                     IPDTHSFEALREAGQAFEAGRVIGR"
     gene            complement(371..1282)
                     /locus_tag="PKM27_18395"
     CDS             complement(371..1282)
                     /locus_tag="PKM27_18395"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNR09297.1"
                     /translation="MKPSLIILAAGMGSRYGGLKQLDQFGPNGEAIIDYSLYDALAAG
                     FGKIVFIIRESFKADMMKAFAHKLDGKVEYHFVNQDLSMIPPGIPVHPERTKPWGTAH
                     AVWVAREVIREPFGVINADDFYGAESYQTLADFLKGLEPDDQRSYCTVGYYLRNTLSE
                     HGTVNRGICSVDEDNNLLAVHERTKIKRFPDGIIRYDSESEHPQPLKDDDFASMNMWG
                     CVPHYFEVMDKHFRIFLQEHGMDPKSEYYIPTLIDYMIAEQLATVKVLPSESTWFGVT
                     YPEDKPAVMAALNKLIGEGKYPSSLWS"
     gene            complement(1299..2654)
                     /locus_tag="PKM27_18400"
     CDS             complement(1299..2654)
                     /locus_tag="PKM27_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNR09298.1"
                     /translation="MKRKTFIKSSSAALAGSYLLPWLSACKSQPMNSLNWAGNYTYKA
                     KRRLTPDSVDELTELVRKERAAKALGTRHCFNDIADSPVSQISTANLNRIRDLDASRL
                     TVTVESGIKYGQLCPYLEERGYALHNLASLPHISVGGAVATATHGSGIKNGNLSSAVR
                     AMELVNASGEVVPISPDSQPEAFYGGVVGLGGLGIVSALTLCIQKSFRMQQRVYQYLP
                     LQSLKENFLPILSAGYSVSLFTDWMKQEVSQVWVKSVYDPMNTGITDFYGAKPATKNL
                     HPIEALDAVNCTEQLGEAGTWYDRMPHFKMGFTPSSGEELQAEYFVPLENGLDALLAL
                     EKKKELIYPLLQISEIRTIDADPFWISPCSDKPSLAIHFTLKPDWPSVQKLLPVIEAE
                     LAPFGAKPHWGKLFTLDAGTLQSRYEKMGDFVHLLRTFDPEGKFRNHFLDTFIYSFEH
                     K"
     gene            2800..4584
                     /locus_tag="PKM27_18405"
     CDS             2800..4584
                     /locus_tag="PKM27_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNR09299.1"
                     /translation="MVLGWLDWAIIIAFLLFSLWIGIWASKSAGKSADEFFLSGRNMP
                     WWLLGFSMVATTFSCDTPNLVTGLVRKEGVAGNWAWWVFLITGMTTVFIYSKLWRRSK
                     VVTDLEFYEMRYAGKMATFLRGFRSIYLGVVFNIIIMGTVSLAVIKMGSIMLGLEGWK
                     TLLILNVITVIYSAIGGLKGVLYTDFFQFIIAMIGSIGAAYYVTTLPEIGSLSNLLNH
                     PNVSGSLNLLPDFSNRELMIGILIIPLLVQWWSVWYPGAEPGGGGYIAQRMLAAKDER
                     NAMGATLLFNIMHYAVRPWPWIIVALASMIIYPEVSDIHNAFPQLPEGKLGDDLGYPA
                     MLTKLPTGLLGIIVASLVAAYMSTISTQLNWGSSYVCNDFYRRFINKHASEKQLVAAG
                     RWTTVILMIFATIMGLMLQDANQGFDLLLSIGAGTGSIFLLRWFWWRVNSYSELAGMV
                     ISFAYALFNSFVLANPLTGSINILVGVLITTAGWLIVTFLTRPEPQETLVRFYKFIKP
                     AAFGWKPVINLAVKEGIIEEDQVETGQLPLEITCVLLGTVTVYSALFSVGFLIYGRIP
                     SFLIALVLTLAGGWYLSRNWGRLKADVL"
     gene            complement(4824..5203)
                     /gene="ssrA"
                     /locus_tag="PKM27_18410"
     tmRNA           complement(4824..5203)
                     /gene="ssrA"
                     /locus_tag="PKM27_18410"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5374..6717)
                     /locus_tag="PKM27_18415"
     CDS             complement(5374..6717)
                     /locus_tag="PKM27_18415"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR09300.1"
                     /translation="MNELKLNTSFSDILRFAIPISFSILIPQINYIVNNIFLSRLGEL
                     ELGTAGITGVYYLVFAVIGFGFNSGLQSLLSRSAGSEKRSDIGRLISQALRMILFTAV
                     FGIAVTYLLAPFVLKSQVSSQEVLQSAIDFIRIRIFGLPFLYAYQVGNSFLISTNNTR
                     FLVIGSVCEAATNILLDYAFIFGHFGFPAMGFNGAAWASVCAEAIGMAVVHSFLWYRR
                     LPQTFSLLAFRRWDWASAWRIVDRSAPLIFQVLISIISWLLFYLWIEHLGQRALAISN
                     TMRNVFGIFGVAIWALASTSNNMVSNLIGQERLDEVIPLIKKIGTLSFLISLPFCLIL
                     NLFPGYFFQIYHSSTGFTHDAIPVIRVVSLAILIMSQGAIWLNAISGTGLTRINLGIE
                     ILAVVVYVIYVFKVVQFEHYPLIWAWGSEFFYWGILFTFSWIFFNWYPWKLKYKK"
BASE COUNT         1766 a         1804 c         1829 g         1798 t
ORIGIN      
        1 cgcccgatca cccgccctgc ttcaaaagcc tggccggcct cccgcaacgc ttcaaaacta
       61 tgggtatctg gaatgaattt cagcaccctc cagtaattgc cccccgaaag catatagtcg
      121 gccccctccc tggttttgta aaggctgaca aagatctccg aagggaaatg ctgggccaca
      181 tgctggctta cctttttcag gttgttcatc agggccggaa catccttaaa cacctggtgg
      241 ttgatccgct ggagcagccc ttccctgccg tttatcctga cccggtaggt atcgttgatc
      301 aggccattgc caaaacgaac cggctgcggg tcccggtcca gtttataagc ttcgaatatt
      361 tcatcttcga tcaggaccat aggctggaag ggtattttcc ttccccgatg agtttattta
      421 aagcagccat cactgcaggc ttgtcttcgg gataggtcac tccaaaccag gtagattcgg
      481 atgggagcac cttgacggtc gccagttgtt ccgcgatcat gtagtcgatc agtgtgggga
      541 tataatattc ggatttggga tccattccgt gttcctggag gaagatcctg aaatgtttat
      601 ccatgacctc aaaataatga ggcacacaac cccacatgtt catggacgca aaatcatcat
      661 ccttcagggg ttggggatgc tccgattcgc tgtcataccg gatgatgccg tccggaaaac
      721 gtttgatttt ggtgcgctcg tggacggcaa gcaggttgtt gtcttcatcc acagaacaga
      781 tcccccggtt gaccgtgccg tgttcggaca aggtatttct caagtaatat ccgactgtgc
      841 aataagaacg ctggtcatcc ggttccaatc ctttaagaaa gtcggccagg gtctgataag
      901 attccgctcc gtaaaaatca tcggcattga tcacgccaaa aggctcccgg atcacctcgc
      961 gggctaccca gaccgcatgg gcagtccccc agggtttggt ccgttccgga tgcaccggga
     1021 tgcccggagg gatcatggaa aggtcctggt ttacaaaatg gtactcgact tttccgtcca
     1081 gtttgtgcgc gaaagccttc atcatgtctg ccttgaaact ttcccggatg ataaacacaa
     1141 ttttgccaaa gccggccgcc agggcgtcgt acaaggagta atcgatgatg gcttcaccat
     1201 tgggcccaaa ctggtccaat tgtttcaatc cgccgtaacg gctgcccata ccggcggcca
     1261 ggataatcaa tgatggtttc attcttttat agctttaatt atttgtgttc aaaagaatag
     1321 ataaaggtat caaggaaatg gttcctgaac tttccttccg ggtcaaaggt ccggaggaga
     1381 tgcacaaaat cccccatttt ttcatacctg gactgcaaag tcccggcatc cagggtaaag
     1441 agtttccccc aatggggttt ggctccaaat ggcgcaagtt ccgcttcgat caccggcaga
     1501 agtttctgca cactgggcca gtccggtttc agggtaaaat ggatggcgag gctgggttta
     1561 tcactacaag ggctgatcca aaaggggtcg gcatcgatgg tcctgatttc cgaaatctgc
     1621 agtaaaggat agatcaattc cttttttttc tccagggcca gcagggcatc caggccgttt
     1681 tcgagaggta caaaatactc cgcctggagt tcctccccgc tgctgggtgt gaaccccatt
     1741 ttaaaatgcg gcatccggtc gtaccaggtc ccagcttctc ccaattgttc ggtgcagttc
     1801 acggcatcca gggcttcgat cgggtgcagg ttttttgtgg ccggctttgc cccataaaaa
     1861 tcagtaatgc cggtattcat gggatcatag actgatttaa cccatacctg gctcacttcc
     1921 tgtttcatcc agtcggtgaa gaggctcaca ctgtatcctg cagacaatat gggtaaaaaa
     1981 ttttccttca aggattgcag gggaaggtat tggtacaccc gttgctgcat cctgaaggat
     2041 ttttgaatgc ataaggtcag ggccgagaca atgcccaggc cacctaatcc caccacccca
     2101 ccataaaaag cctcgggttg ggaatccggt gatattggaa cgacttctcc agatgcgttg
     2161 accagttcca tggcccggac ggcggaggaa aggtttccgt ttttaatacc cgatccgtgg
     2221 gtggccgtgg ccacggcccc gccaaccgag atatggggca aggaggcaag gttatggaga
     2281 gcatagcccc tttcttcgag gtaggggcat aattggccgt atttgattcc cgattcaacg
     2341 gtcacggtca agcgggatgc atccaggtcc ctgatccggt taaggtttgc ggtggagatc
     2401 tggctcacgg gggaatcggc aatgtcatta aaacaatgcc tggtgcccag cgctttggca
     2461 gccctctctt tgcgaaccag ttcggtcaat tcatccacgg agtctggggt caaccgcctt
     2521 ttggctttat acgtgtaatt tccggcccag tttaaagagt tcatgggttg ggatttgcag
     2581 gcagacaacc agggaaggag gtaactgccg gcgagggcgg cagatgagga tttaataaat
     2641 gttttccttt tcataccttc cggcccggtt tcattgaatg ataaaatggc aatgttcctg
     2701 attttacaac cgttcaggat ggaaaatctt ttttaattca atgtaaataa ctggaatatt
     2761 tcaataaatt catttttcaa tttaaaataa ttttctctca tggtattagg ctggctggat
     2821 tgggcgatca tcattgcatt tttattgttt tcgctttgga ttgggatctg ggcttcaaaa
     2881 agcgcaggaa aaagcgcgga tgagtttttc ctttcaggca ggaatatgcc ctggtggctc
     2941 ctcggtttct ctatggtggc caccaccttc tcctgcgata ccccaaacct ggtcaccggc
     3001 ctggtccgga aagaaggcgt agcaggcaac tgggcctggt gggtgttcct gatcaccggg
     3061 atgaccacgg tttttattta ttccaaactc tggagaaggt cgaaagtggt caccgacctc
     3121 gaattttatg aaatgcgtta cgccggtaag atggcgacct tcctgagggg attccgttcg
     3181 atctacctgg gtgtggtatt caacatcatc atcatgggga ccgtatcgct ggccgtgatc
     3241 aaaatggggt ccatcatgct tggactcgaa ggctggaaga ccctgctcat cctcaatgta
     3301 atcacggtca tttacagtgc catcggtggg cttaaaggag tgctttacac ggatttcttc
     3361 caattcatca ttgctatgat cggatccatc ggagcggcct attatgtaac taccctgccc
     3421 gaaatcggga gcctttccaa cctgcttaat catcccaatg tctcgggatc cctgaatctg
     3481 cttcccgatt tttcaaaccg cgaactgatg atcggcattc tcatcattcc cttgttggta
     3541 caatggtggt cggtctggta ccccggcgcc gaacccggag gaggcggata cattgcgcaa
     3601 cggatgctgg cagccaaaga cgaacgcaat gcgatgggcg ccaccctgct gttcaacatc
     3661 atgcattacg cagtaaggcc ctggccatgg atcattgtcg ccctggcctc catgatcatc
     3721 tatccggaag tgagcgatat tcacaacgcc ttcccgcaat tgccggaagg taaacttggc
     3781 gatgacctgg gttatccggc catgctgacc aaactgccca ccggcttgct gggcatcatc
     3841 gtcgcttcac tggttgccgc atatatgagt accatatcga cccagctcaa ttggggttca
     3901 tcatacgtct gcaatgattt ttaccggaga ttcatcaata agcacgcttc agaaaaacaa
     3961 ttggtcgccg caggcagatg gactacggtc atcctgatga tttttgccac catcatgggc
     4021 ctgatgttgc aggatgccaa ccaaggattc gatcttttat tgtccattgg cgcgggtacc
     4081 ggatcgatct ttctgttgag gtggttttgg tggagggtca acagttatag tgaattggca
     4141 ggtatggtga tttcatttgc ctacgccctg tttaattcct tcgtcctggc caatccgctc
     4201 accggttcca tcaatattct cgtgggcgtt ttgatcacca ccgccggttg gttaatagtc
     4261 acatttttga cccgtcctga gccccaggaa accctggtcc gtttttacaa attcatcaaa
     4321 ccggccgctt ttggctggaa accggtaata aatcttgccg tcaaagaagg aattattgaa
     4381 gaagaccagg tggagacggg gcaattgccc ctggagatca cctgcgtgct cctgggcacg
     4441 gtcaccgtct attccgccct cttttccgta ggctttctaa tctatgggag aataccaagt
     4501 ttcctgatcg cgctggtctt aaccctggca ggaggatggt atctatcgcg gaattggggc
     4561 aggttgaagg cggatgtgtt gtgaaggtgg ttggtggata gtgggtggtg ggtggtactg
     4621 ccctctcccg ggtcctcaat cctcaatcct caatcctcaa tcctcaatcc tcaatcctca
     4681 ataagtggag ctggggagat tcgaatggcg aggctgcgct cgccatcgcg ggcgtaagcc
     4741 ccgcgactcc catagtggag ctggagggat tcgaatggcg aggctacgct cgccatcgcg
     4801 ggcgtaagcc ccgcgaccct cgtgagtgga gctggggaga ttcgaactcc cgtccagaca
     4861 aagcgctcat cagctttcta catgcttagt ttccagtttg aatctcggtg cagggcagga
     4921 aagaaaacgt tcccatgtct tcacccagtc tccggttaat ttcatccttt cgccggtgac
     4981 atcgcgaaag gactaccctg agctcccgaa ggagggtttg aatgcgactg gccctgtatc
     5041 aggggtcaga ccggcggcat aaagcggact aattcactga attaggctgc gatagcataa
     5101 gtgttgccac ttaaaggttg aaagctattt ttaacggagt aaactctcat gctccggcat
     5161 gcttactgac cagcaaacct tcctgtcgat tccagtcagc cccatatttc aatgagctat
     5221 cagctatcag ctatcagcta tcagctatca gctatcagct atcagctatc agcttaaaac
     5281 caaattattt cattcagagt tcaatggttc agaataaatc taacccattc tttaggacaa
     5341 ggtattcatt ctattgatac acaagggaat actctacttt ttgtatttca gcttccaggg
     5401 ataccagttg aagaaaatcc aggagaaggt gaaaaggatc ccccaataaa agaactccga
     5461 accccaggcc cagatcaggg gataatgctc aaactgaacc actttaaaaa catagatcac
     5521 atataccacc acggccagga tttcaatgcc cagattgatc ctagtaaggc cggtcccgct
     5581 gatggcattg agccagatgg ctccctggga catgatcaga atggccaggg atacgacccg
     5641 gatgaccggt atggcgtcat gggtaaagcc ggtcgatgaa tggtagatct ggaaaaaata
     5701 gcccgggaaa aggtttaaaa tcagacaaaa aggaagggag atcagaaaac tgagggtccc
     5761 gatttttttt atcaggggaa tgacctcgtc cagcctttcc tgaccgatca gattgctgac
     5821 catattgttg ctggtggagg ccagcgccca gatcgccacc ccgaaaatcc caaacacatt
     5881 ccgcatggta tttgaaatgg caagggcccg ctgaccgagg tgctcgatcc aaagataaaa
     5941 gagcagccag ctgatgatgc tgatcaggac ctggaagatc aggggtgccg agcggtcgac
     6001 gatacgccag gcggatgccc agtcccagcg cctgaaagcc agaagactga aggtctgagg
     6061 taatctcctg taccacagaa aactatgcac gactgccata cctatcgctt cagcacatac
     6121 tgaagcccag gccgccccgt taaaccccat ggccggaaag ccaaaatggc cgaaaatgaa
     6181 ggcgtagtcc agcaggatat tcgtagcggc ttcacacacc gaaccgatca ccaggaaccg
     6241 ggtattgtta gtactgatca ggaatgaatt accgacctgg taggcataca ggaaaggaag
     6301 gccgaaaatc cggatccgga taaaatcgat ggccgattgc aggacctcct gggaggatac
     6361 ctggctcttg agcacaaaag gggccagcaa ataggtcact gcgataccga aaaccgcggt
     6421 aaaaaggatc attcgtaagg cctggctgat cagccggccg atatcgctcc gcttttcaga
     6481 accggcactc ctggacagca gggattgcag tccactgttg aacccaaatc cgatcaccgc
     6541 aaaaaccagg taatagaccc cggtgatgcc cgccgtaccc aattccagct ctcccaggcg
     6601 gctcaggaaa atattgttga cgatataatt gatttggggg atcaggatgg agaaagaaat
     6661 gggaatagca aagcgcagga tgtcagaaaa agaagtattt aatttgagtt cgttcatggc
     6721 gctccggtca tccggcgggg ggcaaatatg agaagaaatc ttcgaaagat ctgactccgg
     6781 aactaagtcg accgtcttgc ccgccaatca agatcttcct ttgttcagtg ggaatattat
     6841 cgcaccagtg gccgaccagg ccaccccttc gtgttcttcg cttcgctgtg tcgtagtggg
     6901 aaatagatgg gccggctggc taatcggtac aagactatcc ttgcttcggt ttatactatt
     6961 tcataaccgc agagtggccg aacaggccac cccttcggcc ttatgtaccc tcttatataa
     7021 tctaagtcca aacaatttga taatcagcat tttaaaataa agggaggtta ataagcacta
     7081 cgtgactttg tgtcgttgtg ggaaattatt gatgtcgttg tgggaaaatt ttttgtgtcc
     7141 ttcagtaagc acccggccaa ccacatattg atcaaggcaa gagtggggca tagattt
//
LOCUS       DAOQXT010000168         7079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_10998_length_7079_cov_6.153474, whole genome shotgun sequence.
ACCESSION   DAOQXT010000168 DAOQXT010000000
VERSION     DAOQXT010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10998_length_7079_cov_6.153474"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..760)
                     /locus_tag="PKM27_18420"
     CDS             complement(164..760)
                     /locus_tag="PKM27_18420"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNR09301.1"
                     /translation="MNLIDLFQGQLSPEVLQQIGNQIGVQDTRQIAAASNGIASTLMA
                     ALNKNTANPSGLNALVSALDRDHDGGILDDLMGYVTGQRQPANASTANGSGILSHILG
                     GMQTNVSDMISRVSGLDRSKVAQLATILAPIILGLLGKQRQQSNVSVGGIGDLLKQTV
                     QTTSQPQMGLLGKFLDRNGDGSVIDDIARMGMDFFLKK"
     gene            944..1762
                     /locus_tag="PKM27_18425"
     CDS             944..1762
                     /locus_tag="PKM27_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNR09302.1"
                     /translation="MTKPQILRNILIGIGALIFAMLIFSNVFVTIQPGHKGIVFRPFG
                     KGLIKDRIFNQGFHIVAPWNRMYVYDVRIQEAFESMEVLSRNGLGIKVDLSFRFSPVQ
                     EKIGYVHDEIGTSYADKIIKPEIRSATREVIGKYLPEELYSSKREAIQDEIFTRSLEE
                     IAKKNLLLDAILIRQVELPQTLRDAIERKLKEEQSSLEYEYRLDKERKEAERILIEAN
                     GKAKANQVLSNSLTDKILTDKGIEATLQLANSPNTKVIVLGSGKDGLPLILNDK"
     gene            complement(1859..3301)
                     /locus_tag="PKM27_18430"
     CDS             complement(1859..3301)
                     /locus_tag="PKM27_18430"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNR09303.1"
                     /translation="MTRMKITGLSTLFALLITQTINAQVGRPFIHDPSTIAECAGKYY
                     TFGTGGGGLISEDGWTWYGGGVRPGGGAAPDVIKIGGRYLVIYGATGGSGNHKGAILT
                     MWNKTLDPKSPDFKYSEPVVVATSDGYEENDAIDPGVMLDPVTGRLWLTYGTYFGFIR
                     LIELDPKTGKLKAGNLPVDVAIACEASTLVYRDGWYYLLATHGSCCDGANSTYNVVVG
                     RSKKITGPYLDNVGRNMLEGGGKMVVATRGGLIGPGHFGHLILEKGVEKMSIHYEADL
                     EQGGRSVLGILPLVWKDGWPVAGEKFKEGTYEIESVRRGYGLELAVDFTRMAGGPRGF
                     FPATNEPIKPVAAQQLGEVMKNWPTGNINLRIGDYMSRPHQKWTITDAPDTTGYLGGP
                     YYKIVLAGTDRALAATADAEIITVPAYTGAPEQLWRIDQLTDGTYRIMPKAVPNSEKK
                     WALVSSGDSTPTLAEFDFNSDNSKWNFRSH"
     gene            complement(3322..>4094)
                     /locus_tag="PKM27_18435"
     CDS             complement(3322..>4094)
                     /locus_tag="PKM27_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09304.1"
                     /translation="KDSAMNRKHKFLWPVIFFIVLSGLQTGGINVFAQGPGPGQRLPL
                     PPIPIKGDTTHTLSKHFTPSSAVKKAPDAKGFIQRWLVLEPVKKDIVRNNIFTDDYLK
                     TAFSTDNFSADYTTIPKNGEMVKLGNHELKWYALDSKAFNFNLYHFTYAINKPNYGIL
                     VWLVTVINCTEEIKNVRLAAGCNSGCQWWLNGREALMLSGDRDMIADNGTSNRLTLKK
                     GKNILRGAVINGPGMANFCVRFIDEKGTPVKNFSISYE"
     assembly_gap    4095..4194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4195..5474
                     /locus_tag="PKM27_18440"
     CDS             <4195..5474
                     /locus_tag="PKM27_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HNR09305.1"
                     /translation="FDWAIADGKIIKAWGFNHQLPGPVLRAKKGDSLVIKVKNELDEP
                     TVIHWHGIRLPASMDGTGEVQKPIQPGETFEYRFVVPDAGTFWYHSHHNETVQMERGM
                     YGALIVEEDTGLLTDADRVLMIDDMKLNRDNSFKKGNFISNWVERHDGRQGDTLLING
                     EENPVIDMHAGQTERWRFINSSSARYFKLNLDGKAFKMIGTDGGLLEQPVAMTQVLLT
                     PGERVDILAGPFTEGETFAMESLPYDRVTILKAKREPFATVQVGPMKPSLAVIPEVLR
                     KIEPLAPAHAAVTRKIRLSVDPSLKHIIDFQVNGGMHTMDKPVYVGDLQVWEIYNSSL
                     MDHPFHLHGFFFQVLEENGKVPGYLAWKDTINLPPRSKAKIAWMPDNRPGRWMYHCHI
                     LEHHEAGMMANFEVVDPGMGEAFAGQDHHHCKG"
     gene            complement(5519..6613)
                     /locus_tag="PKM27_18445"
     CDS             complement(5519..6613)
                     /locus_tag="PKM27_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNR09306.1"
                     /translation="MAKYIYQRKNWTNFSWQDKVINVVFGEVRLMQGKIIGQMNALGF
                     SAKEEATLTALTLDVVKSSEIEGEQLDYDQVRSSIARRLGINTAGLVPGNRHIEGIVE
                     MMLDATQRYTLPLTEKRLCGWHAALFPMGYSGPYKIESGRYRTGIMQVVSGPMGKEKV
                     HYEAVEPKFVKAEMDKFLDWFNYDHTLDPVLKAAIAHFWFIIIHPFDDGNGRIARALT
                     DLLLARAENSSERFYSMSSQILIERKQYYAILQKVQYSAGDITEWLAWFLHCLKNAML
                     ATENTTQKILHKADFWKRHEHTAINERQRLMINKMIDGLEGKLQTSKWAKITKTSTDT
                     ALRDIKDLVEKGILQQTKEGGRSANYILTG"
     gene            complement(6855..>7079)
                     /locus_tag="PKM27_18450"
     CDS             complement(6855..>7079)
                     /locus_tag="PKM27_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09307.1"
                     /translation="DWRSPSKDFDNVAYLQKTLPWAKGMSYRNQPTEELTAKMIKMAR
                     EGGYSGWWGIESSGREEIKQGIGLLKKYLV"
BASE COUNT         1677 a         1713 c         1710 g         1879 t
ORIGIN      
        1 gaggcccgat tcagggaatt atttcaactc caggggggaa ggcccgccct tgcatttatt
       61 ggggcccagg catttaacat actgtggacc ctgatctggg cttttcttct tttcggaggc
      121 cttttattgc cggcacccga tatccggtga aaggcggtaa ggcttatttt ttcaggaaaa
      181 aatccatgcc catccgggct atatcatcga tcacacttcc atccccgttg cggtcaagaa
      241 attttccgag caggcccatt tggggctggc tggtagtctg aacggtttgt ttcagcagat
      301 cgccgatacc cccgacgctg acattggatt gttgcctttg tttgccgagc agccccaaga
      361 tgatgggggc caggatggtt gccagctggg cgactttgga acggtccagc ccgctcaccc
      421 tgctgatcat atccgatacg ttggtctgca tgccccccag gatgtggctg aggatgccgc
      481 tgccgttggc ggtggaagca ttggcgggtt gccgctgacc cgtgacataa cccatgaggt
      541 catccaggat tcccccgtcg tggtcgcggt ccagcgctga aaccagggca ttcagtccgg
      601 agggattggc cgtattttta ttcagggccg ccatcagggt ggaggcaatg ccgttggagg
      661 cggcggctat ctggcgggtg tcctggacac cgatttgatt gccgatttgc tggagcactt
      721 cagggctcaa ttgcccctgg aaaaggtcga tcagattcat atatgctaaa tgagaaaatt
      781 agtttgaata atgctttaca cgggcgattg gggctgtggc aaagatatca aaaattatgt
      841 tgcgggcgat ggattcataa gaccgccttg cccggtttcg ggagaaatgc ttccggatgc
      901 aaccatcccc cgtactttgg tgttagttta ctaaatttcc atcatgacca aaccccagat
      961 cctgcgcaat atcctgattg gcatcggcgc tctcattttt gccatgctga tcttctccaa
     1021 tgtatttgtc accatccagc cgggccataa aggaattgtt ttccgacctt ttgggaaggg
     1081 actgattaag gacagaattt tcaaccaggg tttccatatc gtggccccct ggaacaggat
     1141 gtatgtctac gacgtccgga tccaggaagc atttgaatcc atggaagtgc tttcccggaa
     1201 cggcctgggc atcaaggtgg acctgtcttt ccgcttcagt ccggtccagg agaagatcgg
     1261 ttatgtccac gatgaaatcg gcacttctta tgccgacaaa atcatcaaac ccgaaatccg
     1321 gtcggccacc cgggaagtca tcggcaaata ccttcccgaa gaactttact ccagcaagcg
     1381 ggaagccatc caggacgaga ttttcacccg gtccctggaa gaaattgcca aaaagaacct
     1441 gctgctggat gccatcctga tccgccaggt ggaattgcct cagacccttc gggatgccat
     1501 tgagcgcaaa ctgaaggagg aacaatcttc actggaatat gaataccgcc tggataaaga
     1561 acgtaaagaa gccgagcgaa tactgatcga agccaatggc aaagcaaaag ccaaccaagt
     1621 gctgtccaac agcctgacgg acaagatcct taccgataaa gggatcgaag ccacgctcca
     1681 actggccaat tcacccaata ccaaggtgat tgtgttggga agcgggaagg atgggctgcc
     1741 gctgattttg aatgataagt gagaagcgag aagcgagaaa ccaaacttta tctgaagaga
     1801 agaacccgaa agctacacat ctctatccta ttacataaac attgtgttgg aggtggtctc
     1861 agtgagacct gaaattccat ttagaattat cgctgttaaa atcaaattct gccagggtgg
     1921 gtgtgctgtc cccggaagag accaaagccc acttcttttc tgaattgggc actgccttag
     1981 gcattatccg gtaagtacca tcggtcaatt gatcgattcg ccataactgt tcgggggcgc
     2041 cggtgtatgc cggtacggtg attatttccg catctgccgt tgcagccaat gcccggtctg
     2101 ttcccgctag cacaatttta taataaggac ctcctaaata cccggtggta tccggagcgt
     2161 ccgtaatcgt ccatttttgg tggggacggg acatgtagtc gcctattctt aaattaatat
     2221 tgcccgttgg ccagtttttc ataacctcgc ccaattgctg agccgcaacg ggttttatcg
     2281 gttcattggt ggccgggaag aatccccgcg gtccccctgc cattcttgtg aaatcaacgg
     2341 ccagttctaa tccgtatcct cttcttacgg attcaatttc ataagtgcct tccttaaact
     2401 tttctcccgc aaccggccat ccgtctttcc agaccaacgg gagtatgcct aatacgctcc
     2461 ggcccccttg ctctaaatcc gcctcataat ggatggacat tttttcaact cctttctcaa
     2521 gaataaggtg tccgaaatgc ccggggccta ttaatcctcc gcgggtagca accaccattt
     2581 taccgccccc ttccaacatg tttcttccta cattatcgag gtaagggcct gttatttttt
     2641 tggaacgacc caccaccaca ttgtaggtag agttggcgcc atcgcaacaa ctgccatgcg
     2701 tggccagcag gtagtaccaa ccgtcccgat agactaaagt gctggcttca caggcgatgg
     2761 ctacatcgac cggtaggtta cctgccttga gttttcctgt tttggggtct aattctataa
     2821 ggcgaatgaa tccaaaataa gtgccgtagg tgagccaaag ccttccggtt acaggatcca
     2881 gcatcacccc ggggtcgatg gcgtcgttct cttcatagcc atcggacgtg gccacaacca
     2941 ccggttcaga atacttaaaa tcaggggact tcgggtccag ggtcttgttc cacatagtaa
     3001 gaatggcacc tttgtggttg cctgaacccc cggttgcccc gtatattaca aggtaacggc
     3061 cgcctatttt gatgacatcc ggggccgctc caccacccgg tcttaccccg ccgccatacc
     3121 acgtccaacc atcttcggaa attaatcccc caccgcccgt gccaaaggtg taatacttgc
     3181 cggcgcattc agcgatggta gagggatcat ggataaatgg tcttccaacc tgagcattta
     3241 tggtttgggt gatcaataag gcgaataagg tgcttaggcc ggttattttc attcttgtca
     3301 tgaaatgata ggttttaaat attattcgta actgatgctg aagtttttta cgggcgttcc
     3361 tttttcatct ataaaacgaa cacagaaatt ggccataccc ggtccgttga tgactgcccc
     3421 gcggaggatg ttctttccct ttttaagcgt taatcggttg gaggtgccgt tatctgcaat
     3481 catatccctg tccccggaca gcattagtgc ttcccggcca ttcagccacc attgacaccc
     3541 ggaattgcat ccggctgcca gtctgacgtt tttgatttct tccgtgcaat tgatcacggt
     3601 gaccaaccag acaagtattc cgtagtttgg cttgttaatg gcgtaggtaa aatggtataa
     3661 attaaagttg aaggccttgc tgtccaaagc ataccatttc agttcgtggt ttcccagttt
     3721 taccatttcg ccgtttttgg gaatggtggt gtaatcggcc gaaaaattat cggttgaaaa
     3781 ggcggtttta aggtagtcgt cagtgaaaat attgttcctg acaatatctt tttttacggg
     3841 ttccagcacc agccaacgtt gaataaatcc cttggcatcc ggtgcttttt ttacggcgga
     3901 ggacggcgtg aagtgtttgg atagggtgtg ggtcgtatct ccttttatag gtatgggagg
     3961 gaggggaagt ctttggcccg gccccgggcc ctgtgcaaaa acgttaatcc cccctgtttg
     4021 taatcccgat aagacgataa aaaatataac cggccacaag aatttgtgtt ttctgttcat
     4081 agcagaatcc ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncatttg
     4201 actgggcaat tgccgatggt aaaattataa aagcctgggg ttttaatcac cagctgcccg
     4261 gaccggtact aagggcaaaa aaaggagata gcctggtcat taaagtaaaa aacgaactgg
     4321 atgagcctac cgtgattcac tggcatggga tcaggctgcc ggcttcgatg gatggaacgg
     4381 gtgaagtgca aaaacccata cagccgggag aaacgtttga gtaccgtttc gtcgtgcccg
     4441 atgccggaac cttttggtat cactcccatc ataatgagac ggtacagatg gaaaggggca
     4501 tgtacggagc cttgattgtg gaagaggata ccggtctgct taccgatgcc gaccgggtat
     4561 tgatgataga tgatatgaaa ctgaacaggg acaacagctt taaaaagggc aactttatca
     4621 gcaactgggt tgaaagacat gatggccgcc agggggatac cctcctgata aacggcgagg
     4681 aaaatcccgt tattgatatg catgccggcc aaacggaaag atggcgtttt attaattcat
     4741 ccagcgcccg gtattttaag cttaacctcg atggcaaggc ttttaaaatg ataggcaccg
     4801 acggcggatt gctggaacaa ccagtggcga tgacccaggt attgctgacg ccgggagaac
     4861 gggtggatat cctggcagga ccttttaccg aaggagaaac ctttgcaatg gaatcgctgc
     4921 cttacgacag ggttaccatt cttaaagcga agcgggaacc ctttgccacg gtgcaggttg
     4981 gccccatgaa accatcttta gccgttatac ctgaagtttt gagaaagatc gaaccgttgg
     5041 ctcctgccca tgccgccgtc accaggaaga tccggctttc ggtggacccc agtttaaaac
     5101 atatcattga tttccaggtg aacggaggca tgcatactat ggacaaaccc gtgtatgtgg
     5161 gtgacttgca ggtatgggaa atctataaca gcagcttgat ggatcaccct ttccatttgc
     5221 atggtttctt tttccaggtg ctggaagaaa atggcaaggt ccccggctac ctggcctgga
     5281 aagacacgat caacctgccc cccaggagca aagccaagat tgcctggatg ccggacaacc
     5341 gtccgggcag gtggatgtat cattgccata ttcttgaaca ccacgaagca ggcatgatgg
     5401 caaactttga agtggtagac cccggtatgg gggaagcttt tgcagggcaa gatcatcacc
     5461 attgcaaagg gtaaataata aatgaatccg gtgatgccgt cttcatgggg atgaggtgtt
     5521 aacctgttaa tatataattt gcacttcttc ccccttcttt tgtttgttgc aaaatgcctt
     5581 tctccaccag gtccttgata tctcttaaag cggtgtcggt tgaggttttg gttattttgg
     5641 cccatttgga agtttgcaat ttaccttcca atccgtcaat cattttgttg atcatgagtc
     5701 gttgcctttc gtttatggcg gtatgctcgt gccttttcca gaagtcggct ttatgcagta
     5761 ttttttgtgt ggtgttttcc gtagcaagca tggcgttttt caggcaatgt aaaaaccaag
     5821 caagccattc cgtgatgtcg cctgcgctgt attgtacttt ttgtaatatc gcgtaatact
     5881 gtttgcgttc tatcaggatt tggctggaca tgctgtaaaa acgttcactg ctgttttcag
     5941 ctcgggcaag caacagatcc gtgagggctc ttgcaattct tccgtttccg tcatcaaacg
     6001 gatgaatgat gataaaccaa aaatgggcaa tggcggcttt tagtacgggg tcaagggtgt
     6061 ggtcataatt aaaccaatct aaaaacttgt ccatttccgc ttttacgaat ttgggttcta
     6121 cggcttcgta gtgcactttt tccttgccca ttgggccgga aacgacttgc ataatgcctg
     6181 tccggtaccg acctgattct attttgtatg gaccgctgta tcccatagga aaaagagctg
     6241 cgtgccagcc gcaaagacgt ttttcagtta aaggcaaggt gtatcgttgg gtagcatcga
     6301 gcatcatttc aacaatgcct tctatatgtc ggttacccgg taccaatcct gctgtgttga
     6361 tgcccaaacg ccttgcaatg gatgaacgca cctggtcata gtcaagctgt tccccttcaa
     6421 tttccgatga ttttactacg tctaaggtta aggcggtaag ggtggcttct tctttggcgg
     6481 aaaagcccaa cgcattcatt tgtccgatga ttttaccctg catcaaccgt acttcgccaa
     6541 atacgacgtt tatgaccttg tcctgccacg aaaaattggt ccagtttttc cgctggtaaa
     6601 tgtatttcgc catttcccca aacccaatat gcggtaaaca tactcatttt tcaccgcaaa
     6661 tttgcggtaa tcgtttgagt ttttcaccgc agaatttaca acccgttgaa agtgactccc
     6721 tgttccggtt gcactgttta agcctcgagg gtggtaaaag gctttacctc cgaagttgga
     6781 ttgaaatatt cgatcttcta ttcgccccgg ctttccttca atgcctgatt ttgaactgga
     6841 tatattaaaa acccctacac caaatacttc ttcaacaatc caatcccctg cttaatctcc
     6901 tcccttcccg aactctcaat tccccaccaa cctgaatatc caccctccct ggccatcttg
     6961 atcatcttgg cggtcagttc ctcggtgggc tgattgcggt aggacatgcc tttggcccag
     7021 ggcagggtct tttgcagata ggcgacattg tcaaaatcct tgctgggact gcgccagtc
//
LOCUS       DAOQXT010000169         6973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_11209_length_6973_cov_6.311362, whole genome shotgun sequence.
ACCESSION   DAOQXT010000169 DAOQXT010000000
VERSION     DAOQXT010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11209_length_6973_cov_6.311362"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..780
                     /locus_tag="PKM27_18455"
     CDS             115..780
                     /locus_tag="PKM27_18455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNR09308.1"
                     /translation="MTIPPPDRKLISYQYICIEGNIGAGKTTLCEMLAEEIDCKLVLE
                     EFAENPFLPYFYKEPQRFALPMELFLMAERFKQLQNKIMHRELFNEYILSDYSFVKTL
                     LFAKNNLPEEEYRLFHDMFEVLSNQIPKPDLMVFLHRSIDNLKDHIVQRARPYEQNIT
                     KDYLQGLQNMYLEYFRNELSFPILLLDIDRINFVENRSHFQEIKNLLSESLGPGLHRI
                     SFH"
     gene            861..3872
                     /locus_tag="PKM27_18460"
     CDS             861..3872
                     /locus_tag="PKM27_18460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNR09309.1"
                     /translation="MKYLLPIVAVFLAWTACKPGPASDQGAASSADSASIHEPGMALK
                     GLETMDSLEVSLFASEPMLINPTNIDIDHRGRVYVCEAYNYRPQFSGVNTRFEGDRIV
                     ILEDVDGDGNADSSKVFYQGSEVNAPLGICVLGNEVLISQSPYIWKFTDTNFDDRADL
                     KEILFQGIQGIQDDHGAHALALGPDGRYYFTLGNAGKTISDKNNKPIMTLSGKTIHPD
                     HFRQGMILRGRLDGTGFEVVGHNFRNNYESAVDAFGNIWQSDNDDDGNRGTRLNYILE
                     YGNYGYRDELTNASWQAYRANIEDSIPLRHWHQNDPGVVPNLLQTGSGSPAGIAVYEG
                     QLIPSLEGTILHAEALHHVIRAYSVSGKGAGKFATIRELMKNSRDTWFRPVDVCVAPD
                     GSVFVADWYDPGVGGNYAGDQARGRIYRIAPKNTGYFRDTFSRGRYPTAPSDLKSPNL
                     STRSLAQLHWRRQGMVAIKALLPLARDTNPVFRARALWILSAADPAFVDTALADHDEN
                     IRIAGLRMARSLGPEATSKAIQRLLTTDHSYSVWRECAIALRELPAIQRISAWNQLAQ
                     KYLVPDRWYLEALGIGADLNWDPIFTAWLKNNPDPIATEAQRDIVWRSRSSMALPLLK
                     QLATEGGVPLKSRLRYFRALDFNPSPLKNKILMEILEERGDSLITDLVVRALDPSILK
                     QNKKANATFIQYLSGLSGGPYLDLVEKFKPVNQNDRLFAMMGDSLAGNSGGRAARAII
                     QMSGWKYLEEKIRQSAEPQLYFNLKAIEGIGTRESLALLESFAKNEAQSQRIRKQAYR
                     SLGKSWAGEDYVLSLLARGEIPDDFLEDAISGPGNAWRLPVRNKALEYSKSSAKSLEW
                     SLASLENLSPDPENGQNIFEQLCTACHRIAQKGTSFGPDLDDIGSKYGKEAILTSITE
                     PSRAINFGYEGIQVILLNGSIILGIRQNETAESLTVKTPGGYLQTIEKKTIRESTPVK
                     QSLMTPDLYKHLSAQELTDLLEYLSLQKKTL"
     gene            3902..4798
                     /locus_tag="PKM27_18465"
     CDS             3902..4798
                     /locus_tag="PKM27_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNR09310.1"
                     /translation="MKNKSRRSALKKLGAGASFMALNPLTLPVRPGSKDQSGSGGPLV
                     LKGNINHSVCRWCYRNIPLEDLAIASKKMGITSIELVGPAEWHILKKHGLHCAMPNGA
                     EIGLDKGFNHKQYHEELFKRYSELIPKVAEAGYDQLICFSGNREGIGEEYGLEQSAAG
                     LRPLLPIAEKHKVTLVMELLNSKVNHKDYQCDHTAWGVALCDKLGSDRFKLLYDIYHM
                     QIMEGDIIATIRKYHSYFSHYHTGGVPGRNEIDESQELNYPVIMKEILKTGFKGYVAQ
                     EFIPARPDALDSLEQGVKICDV"
     gene            complement(4801..5490)
                     /locus_tag="PKM27_18470"
     CDS             complement(4801..5490)
                     /locus_tag="PKM27_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09311.1"
                     /translation="MKSRNAYAARLKRLIRHILFPWMDPYLAALLAYPVGWGLALIHY
                     IAELPLLVLDCLGMPEFFGYLQARFKKDARPLLARECAEATPIFSGLVSLQKIRIDED
                     SRVGTHNGKYAYVSYYCINCLGRLSIPVLMHEIVHVLQFEQAGSSYLFRNLMAHLSKA
                     KYNYGGVKRLRQLLKNPRKLHLLNYEQRADIVSDYYLLLDGMRPEWGSATKEDLPVYR
                     EAILELFGKPG"
     gene            complement(5492..6709)
                     /locus_tag="PKM27_18475"
     CDS             complement(5492..6709)
                     /locus_tag="PKM27_18475"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNR09312.1"
                     /translation="MNSWSIAQRLMHYRSGSFVRFILKLAYLSTALSLSVMIIAVAVI
                     SGFNKEITNKIFGFWGHIQVNSIHSSQLNLSTPIVYDSTFTHTLDTLSGIRHYQRFAY
                     IPAIVTAGEEMEGLIFKGVDPDFDWSFFEKFLVNGSIHELRDSLDRQLLISKSLASRL
                     DLEVGDRCRLHFVIDQDVVSRNFRITGIYNTGLEEYDNKLAIGALAEIRQLAGWTENQ
                     IGGYELFVDRLDEIDALSDQIYENHLGTELYVETIRQKFPSIFDWLNLQSTNQYVIQA
                     LMLLVAVINLVTVLLILILERTRMIGTLKALGMRTWSLKKIFLFHGIQILVRGLIWGN
                     AIGLLVCLIQKYAKVIKLNEADYYLRVAPIHLNPWILLLLNLGTIAVTVLVLWIPSGL
                     VSRISPLKAIGYR"
     gene            6755..>6973
                     /locus_tag="PKM27_18480"
     CDS             6755..>6973
                     /locus_tag="PKM27_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNR09313.1"
                     /translation="MNFIETLRWRVMLQDYTPALPEALDRGMVTGYTGFDPTAPSLTI
                     GNYVQIMLLKHFQLHGHKPIVLMGGATGR"
BASE COUNT         1799 a         1811 c         1783 g         1580 t
ORIGIN      
        1 cctggtcccg ctggcagaaa tcgcaccaaa ttgggtgcat ccgggcctga aaaaaacggt
       61 cgcggaactt cttatggaat gcaaggacaa ccttaaagtt aaaaaatgga gggcatgacc
      121 ataccgccac cggaccgcaa gctgatctct taccagtata tctgcattga aggcaatatc
      181 ggggcgggaa aaaccaccct ctgcgaaatg ctggccgaag aaatagattg caaactcgtc
      241 ctggaggaat ttgccgaaaa ccccttcctc ccctattttt acaaggaacc ccagcggttc
      301 gcactgccca tggagctttt tctcatggcc gaacggttca aacagttgca gaacaaaatc
      361 atgcaccggg aactgttcaa tgagtatatc ctttccgatt attcctttgt caagaccctg
      421 ctttttgcta aaaacaacct tccggaagag gagtaccgcc ttttccatga tatgttcgag
      481 gtgctttcaa accagatccc caaaccggac ctgatggttt ttctccaccg ctccatcgac
      541 aacctgaaag accacatcgt tcaaagggcc aggccttatg aacaaaacat caccaaggat
      601 tatctccagg gccttcaaaa catgtacctg gaatactttc gcaacgaact gtccttcccc
      661 atcctcctgc tggatataga ccgtatcaat tttgtggaga acaggtccca ttttcaggaa
      721 attaaaaacc ttctttccga gtccctgggt cccgggctgc atcgcatcag ctttcattga
      781 cgggtgtgcc ggatttaaag gcctgcgttt aaaggccgcc tgtttttctt attttaggct
      841 ttttaatccg ccctgaataa atgaaatatt tattgcccat tgtcgcagtc ttcctcgcgt
      901 ggacggcatg taagcccggt cccgcctcgg accagggggc cgcatcctct gcagattccg
      961 ccagtatcca cgaaccgggt atggccctca aagggctcga gaccatggat agcctggagg
     1021 tcagcctttt tgcctctgag ccaatgctca tcaacccaac caatatcgac atcgatcacc
     1081 ggggcagggt ctatgtttgt gaagcctata attacagacc ccagttttca ggagtaaata
     1141 cccggtttga gggcgaccgg attgtaatcc tggaggatgt ggatggagac ggcaatgccg
     1201 attcctccaa agtcttttac cagggcagtg aggtcaatgc cccattaggg atctgtgtcc
     1261 tgggcaatga agtcttgatt tcccaaagcc cttatatctg gaagtttacg gataccaatt
     1321 tcgacgaccg ggcggacctc aaagaaatcc tgttccaggg gatccagggc attcaggacg
     1381 accatggggc ccatgccctg gcgctggggc cggatggacg gtattatttc accctgggca
     1441 atgcggggaa gaccatttcg gataaaaaca acaaacccat catgaccctt tccggcaaaa
     1501 ccattcaccc ggatcatttc aggcagggca tgatcctgag aggaaggttg gatggcaccg
     1561 ggtttgaggt cgtcggtcat aatttccgga ataattatga atccgccgta gatgcctttg
     1621 gcaatatctg gcaatcggac aacgatgatg acggcaaccg cggcaccaga ttaaattata
     1681 ttctcgaata tggcaactat ggttaccggg acgaactcac caatgccagc tggcaggcct
     1741 acagggccaa tatagaggac tccatccccc tgaggcactg gcaccagaat gacccggggg
     1801 tggtgcccaa ccttttgcaa accgggtccg gatccccggc aggcattgca gtgtatgaag
     1861 gtcagttgat cccatccctg gaaggcacga tccttcatgc cgaggcgttg catcatgtca
     1921 tccgcgctta ttccgtatcg ggtaaaggag ccggaaaatt tgcaaccatc cgggagctaa
     1981 tgaaaaattc cagggatacc tggttccgtc cggtcgatgt ctgtgtggct ccggatggtt
     2041 ccgtgtttgt agcggattgg tatgacccgg gggtcggagg caattatgca ggggatcagg
     2101 cgcggggaag gatataccga atcgcaccga aaaacaccgg ctatttccgt gataccttta
     2161 gcaggggccg gtatccaacc gctccatcgg accttaaaag tccaaacctg agtacccgca
     2221 gcctggctca gttgcactgg agaaggcagg gcatggtcgc catcaaggcc ttgctccccc
     2281 tggccaggga taccaaccca gtgttccggg caagggccct ttggatcctt tctgccgcag
     2341 acccggcatt tgtggatact gccctggctg accatgatga aaacatccgc attgcgggac
     2401 tgcgcatggc caggagcttg ggcccggaag cgaccagcaa ggccattcaa cgattgctga
     2461 ccacagatca ttcctattcg gtgtggcggg aatgcgccat tgctttaaga gaactccctg
     2521 caattcagag gatatccgca tggaaccagc tggcccaaaa atacctggta cccgatcgtt
     2581 ggtacctgga agccctcggt ataggagctg acctgaactg ggaccccatc tttacggcct
     2641 ggctaaaaaa caatccggat cccatagcca ccgaagccca gcgagatatc gtatggaggt
     2701 caaggtcgag catggccctg cccctgctga aacaattggc tacggaaggc ggggtgccct
     2761 taaaatccag gctgcgttat ttcagagcct tggattttaa tccgtcccct ttgaaaaaca
     2821 agatattgat ggagatcctg gaagagcggg gggatagcct cattaccgac ctggtcgtcc
     2881 gcgcgctgga cccatccatt cttaaacaaa ataagaaagc caatgccacc ttcatccaat
     2941 acctgtccgg cctttccgga ggaccttatc ttgacctggt tgagaaattc aaacccgtga
     3001 atcagaatga ccgcctgttt gccatgatgg gtgacagcct ggcgggcaac agcgggggaa
     3061 gggcggccag ggccatcatc cagatgtcgg gctggaaata cctggaggaa aagatccggc
     3121 aatctgccga accgcaattg tatttcaatc tgaaagccat tgaagggatc ggcacccggg
     3181 aatcccttgc cctgcttgaa tccttcgcga aaaatgaggc tcaaagtcaa aggatccgaa
     3241 aacaggctta ccggagcctg ggcaaaagct gggcaggtga agattatgtg ctcagcctgc
     3301 ttgcgcgggg agaaatcccg gacgattttc tcgaagatgc gatatcgggg cctggcaatg
     3361 cctggcggct tcccgtgcgg aataaggccc tggaatattc aaaatcttct gccaaatctc
     3421 ttgaatggtc cctggccagc cttgaaaacc tcagccctga tccggaaaat ggccaaaaca
     3481 tatttgaaca actttgtacg gcctgtcacc ggattgcaca aaaaggaact tcgtttggtc
     3541 ccgatcttga tgacattggg tcgaagtatg gaaaggaggc catccttacc tccatcaccg
     3601 aacccagcag ggccatcaat tttggttatg aaggaatcca ggtcatcctg ctcaacggtt
     3661 ccataatcct cggcatcaga cagaatgaga ccgcggaatc cctgacggtg aagaccccag
     3721 gcggatattt gcagaccatt gagaagaaaa ccattcgtga aagcaccccg gtcaaacaat
     3781 ccctgatgac ccctgatttg tataaacacc tgagcgccca ggaacttacc gacctgcttg
     3841 aatatttaag tttacagaaa aaaaccctgt aaattggcat atcataatta cgcttgtccc
     3901 tatgaaaaat aaaagcagaa ggtctgccct gaaaaaactc ggcgccgggg cttcctttat
     3961 ggccctgaat cccctgaccc tcccggtaag accaggatct aaagaccaat ccggttccgg
     4021 gggacctctg gtattaaaag gcaatatcaa ccattcggta tgccgctggt gttaccggaa
     4081 tattccgctg gaggatcttg cgattgcctc caaaaaaatg ggcatcacct ccatcgaact
     4141 ggtggggccg gcggaatggc atatcctgaa aaaacacgga ttgcactgcg ccatgcccaa
     4201 tggcgccgag atcggcctcg acaaaggatt caaccataaa caataccatg aagaactgtt
     4261 caaacggtac agcgaattga ttccaaaggt ggcggaagcg ggatatgatc aactgatctg
     4321 tttttccgga aaccgggaag ggatcgggga agaatatggg cttgaacaat cggcagccgg
     4381 cctccgtccc ctgctcccca tcgccgaaaa acacaaggtc accctggtga tggagttgct
     4441 caacagcaag gtcaaccaca aggattacca gtgtgaccat acggcctggg gagtggccct
     4501 ttgcgacaaa ctgggttcgg atcgtttcaa actgctctac gacatctatc acatgcaaat
     4561 catggaaggg gacatcatcg ccaccataag aaaatatcac tcctattttt cacattacca
     4621 caccgggggc gttccgggaa gaaatgagat cgatgaaagc caggaactga attaccccgt
     4681 catcatgaaa gaaattctga aaacgggctt taaaggttat gttgcccagg aatttatacc
     4741 tgcccggccg gatgcgctgg acagcctcga gcaaggggtg aaaatctgcg atgtctgaac
     4801 tcagcctggt ttcccaaaaa gttccaggat ggcctcccgg tagacgggta aatcctcctt
     4861 cgtagcgctt ccccattccg gacgcatccc atccaacaac aggtaatagt ccgagactat
     4921 gtccgccctt tgttcgtagt tgagaaggtg caatttcctt ggatttttca acagttgcct
     4981 cagtctttta acgcccccat agttgtattt ggctttgctc aaatgagcca tgaggttacg
     5041 gaagaggtaa gaagagccgg cctgttcaaa ctgaaggaca tgtacgattt catgcataag
     5101 cacgggaatg ctgagtcgtc caaggcagtt gatgcaataa taactgacat aggcatattt
     5161 gccattatgc gtccctaccc tgctgtcttc atcgattcta attttttgca aagaaaccag
     5221 gccgctgaaa attggggtgg cctctgcaca ttccctggct aggagcggcc tagcgtcctt
     5281 tttgaatctc gcctggaggt atccaaaaaa ttcgggcatc cccaggcaat ccaggaccag
     5341 caaaggtaac tccgcgatat aatgaatgag ggcaaggccc caaccgacag gataagccag
     5401 caaggcggcc agataggggt ccatccaggg aaaaagaatg tgacggatca aacgtttcag
     5461 gcgcgctgca tatgcattcc gtgatttcat gctaccggta gccgatggct ttcaatggag
     5521 agatccggct gaccagcccc gaagggatcc agagaaccag gaccgtgacg gcgatcgtac
     5581 ccagattaag cagtaaaagg atccaggggt ttagatgtat cggggccacc cggaggtaat
     5641 agtccgcttc attgagtttg atcactttgg catatttctg aatgaggcag accaggagac
     5701 cgatcgcgtt tccccagatc aggcctctga ccagtatttg tatgccatgg aaaaggaaga
     5761 tttttttcag ggaccaggta cgcatgccca gggctttgag ggtaccgatc atgcgggtcc
     5821 tttccaggat caggatgagt aaaacggtga cgaggttgat gaccgccacc aataacatca
     5881 aagcctggat gacgtattga tttgtggatt gcaggttgag ccagtcaaaa atacttggga
     5941 atttctgccg gatggtttcc acatacaatt ccgtgcccag atggttttca tagatctggt
     6001 cggagagggc atcgatttca tccagccggt caacgaacaa ttcatatcct ccgatttggt
     6061 tttcagtcca accggccaat tgccggattt ctgccaaagc cccgatcgcc agcttattgt
     6121 catactcttc gaggcccgta ttgtaaatcc cggtaatgcg gaagttgcgg ctgaccacgt
     6181 cctggtcgat gacaaaatgc agccggcacc ggtctcccac ctccagatcc agccgggagg
     6241 caaggctttt ggaaatcaat aactgcctgt cgagcgaatc cctgagttca tgaatgctgc
     6301 cgttgacgag aaatttttca aaaaaggacc agtcaaaatc ggggtcgacc cctttgaata
     6361 tcaatccttc catttcctcg ccggccgtga cgatagcagg gatatatgcg aaacgctggt
     6421 agtgccggat tccggaaagg gtatcgaggg tatgcgtaaa ggtgctgtcg tatacaatgg
     6481 gcgtactgag gttgagctgg gaactgtgga tgctgttcac ctggatatgc ccccaaaagc
     6541 caaaaatttt gttggtaatt tctttgttga agccggagat gaccgcgacg gcgatgatca
     6601 tcacggagag ggacagtgcg gtgctgaggt aagccagttt cagaataaag cggacaaaac
     6661 tccccgaccg gtagtgcatc aggcgctgcg cgatggacca gctattcatc tggggcaaat
     6721 gtcctacttt tgcctcattc aatctggaaa taaaatgaat tttatagaaa ccctgcgctg
     6781 gagggtcatg ctgcaggact acactcccgc tctgccggaa gccctcgatc ggggaatggt
     6841 cacgggatat accggatttg acccgaccgc cccttccctc acgataggca attatgtgca
     6901 gatcatgctg ctcaaacact ttcaattgca tgggcacaaa cccatcgttc tgatgggtgg
     6961 cgccaccggg cgc
//
LOCUS       DAOQXT010000170         6867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_11443_length_6867_cov_6.671609, whole genome shotgun sequence.
ACCESSION   DAOQXT010000170 DAOQXT010000000
VERSION     DAOQXT010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11443_length_6867_cov_6.671609"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1216)
                     /locus_tag="PKM27_18485"
     CDS             complement(89..1216)
                     /locus_tag="PKM27_18485"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNR09314.1"
                     /translation="MEVGYDAKRLFHNHTGLGYYSRTLIQSLSEADQDFKATLFDARP
                     VATAQTELFYDPEKYRIIKPGSPAWYYRSVRINKYLDQTRLNLFHGLSNELPYRPLPA
                     SIPAVVTIHDVLFKSFKADFPWHDRWVYQWKTRVALERADLVVAISQATREDLLRHFK
                     VDEKKIRVIYQSYDPVFDQPVSDSEVAEILANYRLPSEYLLYVGSVTHRKNLMVILRA
                     LQAMEEKNRIPLLVAGKGKDYQKQAVEYIRRHGLESRVFFLPDLPRYVLRILYAGAQM
                     LIYPSLGEGFGLPVLEGIAANIPVITSNRSSLPEAGGEVAHYFNPEDPGELKELIEKV
                     SGTANSTKSDHRRIEHLQKFNKNRIARQYLQEVYGVLVGRR"
     gene            complement(1235..3961)
                     /locus_tag="PKM27_18490"
     CDS             complement(1235..3961)
                     /locus_tag="PKM27_18490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNR09315.1"
                     /translation="MTTQTALPRLVFKASDIDIPYKRYVLSNGLRLIVHEDFKAPIAA
                     VNVWYHVGSKNEKPGKSGFAHLFEHLMFNGSENFNTDYFQALEAIGATDFNGTTNNDR
                     TNYFQNVPIGALDQVLWLESDRMGHLTGAIDQAKLDEQRGVVINEKKQGENQPYARED
                     DLFTEAMFPKGHPYSWTVIGSVEDLEGASVEDVHEWFKNFYGAANACLVIAGAIRAEE
                     ALEKVKKYFGHIPCGPTIARHESNLPKRTGHTRQEYEDHVPEAKVSMAWNVPQWGTRE
                     AAYLDLLAAILSTGKTSRLYKKMIYDQQLATSVSAFNYSREIAGNFYIECKVKTGHTV
                     DEVESTIQSILQELLDQGPSEEELLKVKSQYFAGFIKGIERIGGFGGKSDVLAESEIF
                     GDHPEFYKTYNGFIEKATVQDLKDTANAWLRDGSHTVIARPFPQYATEPTSVDRSRLP
                     DLATTPPSAFPTLQKAQLSNGLRVVLARRKESPTVVFSLMMPGGFSSDQLSGKPGLAS
                     IAMNMLDEGTSSLSALEISEKLSLLGASISSFADLDQSYLTLNTLLPSLRESLDLFAD
                     ILLNPSFPVPDFERLKSLQIAGIQREKTNPIHMALRVMPKFLYGDRHPYSNPMSGSGY
                     EQSVSSITLEDVKQYYDQWIRPGDATLSVVGDLEMPELLGLLETRLAGWKSGKPVIKP
                     STQAPAQKGRALYLMHKPETQQSVLMAGYTISPYGGISEIAREALVNILGGDFTSRIN
                     LNLREDKHWTYGAGVFIRDAILERPFITYTQVQIDKTRESIEEIIKEFDWINGGKPVS
                     REEFEKTRNNQIMALPGIWETNAAVSASLSEQIRFGLPDDYWQSYSERVQALELAEVH
                     ELGKELIRSSWLNWFVVSNKDVVLPSLKELGFDEIILVDADGNRCEPESRLD"
     gene            complement(4039..4509)
                     /locus_tag="PKM27_18495"
     CDS             complement(4039..4509)
                     /locus_tag="PKM27_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNR09316.1"
                     /translation="MLTNKHPNHLRLEAFYKAFALKDADSMNQIYHEKATFYDPVFSN
                     LPPGEVMKMWRMLCSRGKDLIIDFQVLEADEQEGKAKWTAHYTFSATGRKVVNLINSR
                     FLFENGKVTTQKDTFDFYAWARQAFGLKGLLLGWTPWFKNKVRKGALKGLSRFE"
     gene            4626..5045
                     /locus_tag="PKM27_18500"
     CDS             4626..5045
                     /locus_tag="PKM27_18500"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HNR09317.1"
                     /translation="MSRFLHKIKAIFGHRCPRCEKGKMFTEGLFNFSKFLEMPEKCPV
                     CGLHFTPEPGFYMGSMFVSYAVFSWGMLALVAVFYLVFHIGINFSIFLAVLGGLISFT
                     WVIRFARVVALHMVRSFDPNTKAKVEAEVEAEVEAKG"
     gene            complement(5449..5862)
                     /locus_tag="PKM27_18505"
     CDS             complement(5449..5862)
                     /locus_tag="PKM27_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09318.1"
                     /translation="MNSVFPGITGISFLLLVFSCRPAPKHYLDHRWSGDDGNQIMIFD
                     GDSSLQWIFHEPTLSDTFLVRYRLDQSATPNRLDLYNFSSGILKGKILAGIVQPLSPD
                     SIQLDFEPAETWAEADSVRPRTFDPEQRRVFVKVK"
     gene            complement(6413..>6867)
                     /locus_tag="PKM27_18510"
     CDS             complement(6413..>6867)
                     /locus_tag="PKM27_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09319.1"
                     /translation="SCNQKSDDIKVLQTRVDSLEKKLANTYIPGFGEFMSSIQAHHSK
                     LWFAGQYENWKLADFEVHEIMEGIEDIQKFQTERKESQWVGMINPALDSISQAIQMKN
                     PALFKSCYTLLTNTCNQCHREVEFDFNMVKIPDHQPFSNQDFKVHESN"
BASE COUNT         1536 a         1683 c         1825 g         1823 t
ORIGIN      
        1 cttaactacc atggtttgcc gaatatcgct caaatgtaat caaagaggaa gaaatagagg
       61 cgcctcgtga cctcttacct cttacctctt acctccgccc aaccaatact ccataaacct
      121 cctggagata ttgcctcgct atcctgttct tattgaactt ttgcaggtgt tcgatccggc
      181 ggtggtcgct tttcgttgaa ttggccgtcc cgctgacctt ctcaatcaat tccttcaact
      241 ccccggggtc ttcaggatta aaataatggg ccacttcacc accggcttca ggcagggacg
      301 atcggttgga ggtgatcacc gggatattgg cggcaatgcc ttccagtacc ggaaggccga
      361 aaccctcacc cagggaggga tagatcagca tttgagctcc ggcgtatagg atgcgcagca
      421 cataccgtgg tagatccggc aaaaaaaata cgcgggactc cagcccgtgc cggcggatat
      481 actccaccgc ctgtttctgg tagtccttgc cttttccggc aaccaaaagc gggatcctgt
      541 ttttttcttc catcgcctgg agggcgcgga ggatcaccat caggttttta cggtgggtga
      601 ccgaaccgac ataaagcaag tattccgagg gtagccggta attggccagg atctccgcca
      661 cctcactatc cgataccggt tggtcgaata ccggatcata ggattggtag atcaccctga
      721 tttttttttc atcgactttg aagtgccgga gcaggtcctc tcttgtagcc tggctgatgg
      781 cgacgaccag gtcggccctt tcgagagcga ccctggtctt ccactggtag acccaccggt
      841 cgtgccaggg gaaatcggct ttgaaagact tgaacagcac atcgtggatg gtcaccaccg
      901 ccggtatgga tgcgggcagg ggacggtatg gcaactcatt gctgagtcca tgaaaaaggt
      961 tgagcctcgt ttgatcgaga tatttattga tccggacact ccggtaatac caggccggcg
     1021 agcccggttt tatgatccgg tatttttcag ggtcgtaaaa caattcggtc tgggcggtcg
     1081 ccaccggtct ggcgtcaaac agcgttgctt tgaaatcctg atcggcctcg ctcaggcttt
     1141 ggatcagggt gcggctataa tagcccagtc ccgtatggtt gtgaaacaat cgtttggcat
     1201 catatccgac ctccatgaca ggtttatacc gttttcagtc cagccgggat tcaggctcgc
     1261 aacggttccc gtccgcatcc accaggatga tttcgtcaaa gcccagttct ttgagggaag
     1321 gcaggaccac gtccttgttg ctcaccacaa accaattgag ccaggacgac cggatcaatt
     1381 cctttcccaa ttcatggact tcggccagtt ccagagcctg cacccgctca ctgtaggatt
     1441 gccagtaatc atccggaagg ccgaaccgga tctgttcgga gagggaggcg gaaaccgcgg
     1501 cgttggtctc ccagatcccg ggcagggcca tgatctggtt gttccgggtt ttttcaaatt
     1561 cttcccggga aacgggtttc cctccgttga tccagtcaaa ttccttgatg atctcttcaa
     1621 tggattcccg ggtcttgtcg atctgaacct gggtataggt gataaacggc ctttccagga
     1681 tggcgtcgcg aataaaaacg ccggcgccat aggtccagtg tttgtcttcc ctcaggttga
     1741 ggttgatcct tgaagtaaaa tcccccccga gtatattgac cagcgcttcc ctggcgattt
     1801 ccgatatgcc gccgtaaggg gaaatggtat aaccggccat cagcaccgat tgctgggttt
     1861 ccggtttatg catgaggtag agggcgcggc ctttttgggc cggagcttgg gtactgggtt
     1921 ttatcacagg cttgccggac ttccaaccgg ctaacctggt ttccagaagg cccagcagtt
     1981 caggcatttc caggtcaccc actacagaga gggtggcgtc ccctggcctg atccattgat
     2041 cataatattg tttaacatct tccagcgtaa tggaggatac ggattgttca tagccggaac
     2101 cggacattgg attgctgtaa ggatgccggt ccccgtataa aaatttgggc atgacccgga
     2161 gggccatgtg gatggggttg gttttttcac gctggattcc tgcaatctgg agggatttga
     2221 gccgttcgaa atccggtacc ggaaaggaag gattcaagag gatatcggca aacaaatcca
     2281 ggctttccct caggctgggc aaaagggtat tcagggtgag gtaggactgg tcgaggtccg
     2341 caaaagaact gatcgaagct cccagcaggc tgagtttttc gctgatttcc aaagcgctga
     2401 ggctgctggt gccctcatcc agcatattca tggcgatgga agccaggccc ggttttccgc
     2461 taagctggtc gctgctgaag cctccgggca tcatcaggct gaaaaccacg gtgggcgatt
     2521 ctttccgcct ggcgagcacc acacgaaggc cattgctgag ttgtgctttt tgaagggtag
     2581 ggaaggccga gggcggggtc gtggccaggt ccggcagcct ggaacggtcc acgctggtag
     2641 gttccgtggc atactggggg aagggcctgg cgatcaccgt atgggagcca tcccggagcc
     2701 aggcattggc cgtatccttc aggtcttgca cggtggcctt ttcaataaac ccgttataag
     2761 ttttataaaa ttccgggtgg tccccgaaga tctcgctttc ggccagtaca tccgattttc
     2821 ccccaaagcc gccgatccgt tcaatgcctt tgatgaagcc ggcaaaatac tgggatttga
     2881 ctttgagcag ttcctcctca gaaggaccct ggtccaggag ttcctgcaga atggactgga
     2941 tggtggattc cacctcatcc accgtatgcc cggttttgac tttgcactcg atataaaaat
     3001 tacctgcgat ttccctggaa taattgaatg cgcttacgga agtggccagc tgttgatcat
     3061 agatcatttt tttatacaac ctggacgttt ttcccgtgct taatatggcc gcgaggagat
     3121 cgaggtacgc ggcttcacgg gtgccccact ggggtacgtt ccaggccatg gacaccttgg
     3181 cttccgggac atggtcttcg tattcctggc gggtatggcc ggtacgtttg gggaggttgg
     3241 attcatgccg cgcaatggtg gggccgcagg ggatgtggcc aaaatatttt ttcacttttt
     3301 ccagggcttc ttcggcccgg atggcgcccg cgatcaccag gcaggcgttg gccgcgccgt
     3361 aaaagttctt aaaccactcg tgtacgtcct ccacggatgc cccttccagg tcttccacag
     3421 atccgatcac cgtccaggaa taaggatgtc ctttggggaa catggcctcg gtaaacagat
     3481 cgtcttcgcg ggcgtaaggc tgattttcgc cctgcttttt ttcattgatc accactcctc
     3541 tttgttcgtc gagtttggct tggtcgatgg ccccggtgag gtggcccatg cggtccgact
     3601 ccagccagag tacctggtcc agggcgccga tggggacgtt ctggaaataa ttggtccggt
     3661 cgttgttggt ggtcccgttg aagtcggtgg ccccgatggc ctcgagggcc tggaaataat
     3721 cggtattgaa attttcgctc ccgttgaaca tgaggtgttc aaacaaatgg gcgaatccgc
     3781 ttttgccggg cttttcgttt ttggatccca cgtggtacca gacgttcacc gcggcgatgg
     3841 gggctttaaa atcttcatgt acgatcagcc tcagtccgtt ggacaggacg taccgtttat
     3901 agggaatatc gatgtctgag gctttaaata caagtcgggg aagggctgtt tgagtagtca
     3961 tacctgcaaa aaaaatgcaa aaaaaccgga tttatgggaa ttattcccgc ttaagttcac
     4021 ttccggattg cgaatctctc attcaaaccg gctcaggcct ttcagggccc ccttcctgac
     4081 tttattctta aaccagggcg tccagcccaa taaaaggcct ttcagaccaa aggcttgacg
     4141 ggcccaggca taaaaatcaa atgtgtcttt ttgggtggtc actttgccgt tttcaaagag
     4201 gaaccgggaa tttatgaggt tgaccacttt cctcccagtt gcggaaaaag tatagtgtgc
     4261 cgtccatttc gcttttccct cctgttcatc cgcttccagc acctggaagt cgatgattaa
     4321 gtctttgccc cggctgcaca gcatgcgcca catcttcatg acctcgccgg gaggaagatt
     4381 tgaaaatacc ggatcgtaaa aggtggcttt ctcatgatag atctgattca tggaatcggc
     4441 gtctttcaag gcgaaggctt tgtagaaagc ttccagcctc aggtggttcg gatgtttgtt
     4501 agttagcaag taggcaagtt agcaagttgg tggaaaaggt agtacagaaa acgtgcagat
     4561 gttcttgctg actagctaac tagctaactg actaactgat taactgaatc cctatctttg
     4621 caccaatgtc ccgcttcctc cataaaatta aagcaatatt cggtcatcgc tgtccccgtt
     4681 gtgagaaggg gaagatgttt acggaaggcc tgttcaattt ttcaaaattt cttgagatgc
     4741 cggagaaatg cccggtatgc ggcctgcatt ttacccccga gcccgggttt tacatgggat
     4801 ccatgtttgt gtcctacgca gtcttttcct ggggtatgct ggccctggtg gccgtgtttt
     4861 acctggtctt tcatatcgga atcaactttt ccatcttcct ggctgtactt ggtggcctga
     4921 tcagctttac ctgggtgatc cggtttgccc gggtggtggc tctgcatatg gtccggagtt
     4981 ttgatccgaa tactaaggct aaggttgagg ctgaggtgga ggcggaggta gaggctaaag
     5041 gttgaggctg aggttgaggc tgaggtggag gtagaggcta aaggttgagg ctgaggttga
     5101 ggttgaggtt gagggccgtc ctgtaataat gagtcatttc gccttgaacc tggcttgatt
     5161 gatcacagag cgagaaccat aaaggatttt tatcaatgtc aaacttttga ctccccttta
     5221 gggggtgggg gcggagataa atgcaagtcc aaaactttac ccgataattc cttttcgact
     5281 gtctagcccc ctgaccccca cggggggacc aaagcctttg gtagtcctgt aaaaggcgaa
     5341 tcaattcgcc ctgcattcct ccttgattta ccacagagca gctactgaat tttccgtttc
     5401 gctcacttcg ccctttaccc ttcgcctttc gccctttacc cttcgccctt atttcacctt
     5461 gacaaaaacc ctcctttgct ccggatcaaa cgttctgggc cgcacgctat ccgcttccgc
     5521 ccaggtttcc gccggttcaa aatcgagctg gatgctgtcc ggggataaag gctgcacaat
     5581 cccggccagg attttccctt tcaggatccc gctgctgaaa ttatagagat cgaggcggtt
     5641 gggggtggcg ctttgatcca gccggtaccg gaccaggaag gtatcagaca gggtgggttc
     5701 atggaagatc cattggaggc tgctgtcccc gtcaaagatc atgatctggt tgccgtcatc
     5761 gccggaccac ctgtggtcga ggtaatgttt gggcgcggga cggcaggaga atacgagcag
     5821 caggaaggat attccggtga tcccggggaa aacggagttc attttttgac gggatgaaat
     5881 tacaaaaggc aggtacaagg accgaaaaat tattataaaa taaaatttct cctgacgtta
     5941 taggtaccgc atttccaccg gtgttggcaa gcgcaccggc cggagtcgtc ccggcaaatg
     6001 gcaggaaggc ctgtggcgga aattctgtag aacctagggg attaagcata attcccgata
     6061 actttagcaa agaatcaaaa acccaaactt tatgaataat tttttcagag gactgattgc
     6121 gtcctacggc gcttataaac tgggaggcgg ctgtatcgga acaattgtgg tttttatcat
     6181 tatttgggtg gcgctgggat attgcaactg atgcggattc ggtggtataa tgaataaccg
     6241 aaaaagagat cactcccccc ccgtatatcc atttaaaaac cggttcaatc ctttctgctt
     6301 taacagccac acaaggtcct gcaatgcaat agggattaaa ttattaaaaa caggttttga
     6361 ccgtgccata aacggttcat ctcatacaaa gtggataatt ataagttaca gattagtttg
     6421 actcatgcac tttaaagtct tggttgctga agggttgatg gtcgggaatc tttaccatat
     6481 taaaatcaaa ttccacttcc ctatggcatt ggttgcaggt gttggtgagc agggtgtaac
     6541 agcttttgaa caaggctggg tttttcattt gaatagcctg gctgatgctg tctaaagcgg
     6601 gattaatcat tccgacccat tggctttcct ttcgttctgt ttggaatttc tgaatgtctt
     6661 caatgccttc cattatttca tggacttcaa agtcggccag tttccagttt tcgtattgcc
     6721 cggcaaacca cagtttggaa tgatgagcct gtatgctgct cataaattcg ccaaaaccag
     6781 gtatataagt atttgcaagt tttttttcta aactgtcaac gcgggtttgt aaaaccttta
     6841 tgtcatccga cttttgattg caggaaa
//
LOCUS       DAOQXT010000171         6743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_11693_length_6743_cov_7.634420, whole genome shotgun sequence.
ACCESSION   DAOQXT010000171 DAOQXT010000000
VERSION     DAOQXT010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11693_length_6743_cov_7.634420"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PKM27_18515"
     CDS             complement(<1..1143)
                     /locus_tag="PKM27_18515"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNR09320.1"
                     /translation="MQAKSIKGNSTGEIQSALQQCITNDFKPTLAIVFMSVKQDREAV
                     CSLLNEQGIQIFGSTTSGEFISSEISEGGIAIMLLDPDPALFQLVFLETGGSSAYEVS
                     KQLGVLGKNSFAQAAFLVASGWLHTDGERIIKGIEEGYQGEPAIYGGMAGDDLALSGP
                     IVFTQGKSSITGLVAIIIDTDRVEVSGIATCGWKPIGITKTVTKSEGNVVYTMDDQPA
                     LDLVIKYLGLNMDDMNAITNTVFNLGAYYPLQLEREDAPPVMRTAMLGNKEDRSLICA
                     GNVPQGSKVRFSLPPDFDVIDTVVNECSDLKTGNRNGVDAVIMFSCISRYLSFGVMTS
                     EEIERVMGVWNAPFIGFFSYGEYGKSRRGKHEFHNNTCCVVTLKEIR"
     gene            complement(1150..1452)
                     /locus_tag="PKM27_18520"
     CDS             complement(1150..1452)
                     /locus_tag="PKM27_18520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HNR09321.1"
                     /translation="MIKGTVLYGHPKDAAAFEKYYVEKHLPLVAKTQGVLKLELTKFL
                     PNPDGSAPAYYRMAELYFDNAAEMQQALGSPEGQAMAADLANFATGGLTIVFGAVE"
     gene            complement(1464..2642)
                     /locus_tag="PKM27_18525"
     CDS             complement(1464..2642)
                     /locus_tag="PKM27_18525"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNR09322.1"
                     /translation="MKAKSIQGNSLEEVRYALEDILDLTARSGDQEFRPELAFVFVSI
                     KQDWVTLRDLLHRKGLEVLGATSSGEFTEAGQSEGGISVLLLELPKADYAIFYEPIGE
                     RTMEDAAMNLARRALQTFDCPAFILLSTLLSADGKMLEGETLIRSIEKVTGPQVTLFG
                     GMAGDDISFTGTYVFTHDQSTDYGLVALILNEEKVSLSGMAISGWKPVGVARTITKSR
                     GNLLYTIDGQPALDMYLRFLGMERSSAEDQVRFFDTIGVHYPFQIEREGREPMMCNPI
                     GYNKEEGALICESDIREGSRFRFSTPPDFDILETVVGKANELKMQTHAKADALLIFSC
                     AGRLSALGPLARDENEKLAGVWNVPMAGFYTYGEFGKGPNGKHEFHSTTNSWVVLKEK
                     "
     gene            complement(2759..3028)
                     /locus_tag="PKM27_18530"
     CDS             complement(2759..3028)
                     /locus_tag="PKM27_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09323.1"
                     /translation="MEAVFRNRSGRKYFPEGMPLAQPFFTTLPIVIGTTGQATGLGLS
                     LAYDIVKAHGGDIKVISNYFPPSGELGMEKEDGSGSEFIIRLPVG"
     gene            complement(3168..6599)
                     /locus_tag="PKM27_18535"
     CDS             complement(3168..6599)
                     /locus_tag="PKM27_18535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNR09324.1"
                     /translation="MALRICVALAFIFLLNTAVPGQKELNFTYYKETQGVEIWEVLSD
                     QFGRIWLGTSDGLMRFEGYEFKRFVYDPKDSSSIKGAVISTLYEDALGNIWISAFDER
                     LYRFDPRSGSFKTYPFFQLIGKTMENNDFGISTISMDAGGRLYFGANSVSPLPVSLLI
                     YDESGDRIRPFPLPDSANPGGFYLARPDHAKNTLFLGLDNQLFFFKEGKSLSRLGPYT
                     ELQNLANQTSVLDFIFDQEDRLWFMSDQGSLAVFDKLDGAPLKTYSFRHLLPDPSKPL
                     QSCILKLDQNKNLWIGLDKGLIRFDLKNEKSEFFKLPKDHRGKNYEVRSLNLDSFNNL
                     WIGTQNGLLKHEDKPVFINYTSKNDDTASTLKGYAVSLTQAGKAKILVLSYEVGTSIP
                     VLNELDLQTNTIKKHHIGSIFPKGSMVFGVHSTGTDTLTFGTDHGVYRLNLDNKRAQK
                     VSLKGVPNIHPGILQFYTDAFGNEWLGTLSYLYKKPKGSEEFVPMDLSRQPGGTERSN
                     MVRVEDGKSKGLWLLTDNGLYLYDFHTDSITRHGYDKKTGDVFLNQSIYAVVQEDSSD
                     IVWVGMQRGGLNRYNSRDKKIKTYTTEDGLADISVYAILHDEKNRALWLGTGQGISRF
                     DLQSEKFTNFSTEDGLDSKRYYTALKTTDGRFFFSGEELIQFNPDEISLTAVPPLISF
                     GDVKVSIWTLALGWGTRIEKGLTDDASLTLNHDENNITIEYLGGHYSNPAKNRFSYIL
                     ENYDDRWIDVGSQQAAYFTNLPPGKYVFRVKAANSNGIWNEQGIKLNIQILYPWWRTY
                     WAYALYLVGLAGMASVANRYFKQRVLKRERDRTQLKELEQARLIEKAYRELESSHQAL
                     KATQSKLIQSEKMASLGELTAGIAHEIQNPLNFVNNFAEVNQELIGEIEGERTKQKED
                     RNEQLETQLLQTLKENEQKIHQHGQRADAIVKSMLQHSRTSSSKKEPTDLNTLCDEYL
                     RLSYHGLRAKDPDFKAKYELDPDPELPRIDLVPQDMSRVLLNLINNAFYAVNEKRKRL
                     GTAAVNERAKQNISGYEPLVRISTRRNEKGVEIRVKDNGPGIPEQVREKIFQPFFTTK
                     PTGQGTGLGLSLPYDIVKAHGGDIKVISNYFPPSEELGVETDPGSYTEFIIHLPV"
BASE COUNT         1672 a         1695 c         1493 g         1883 t
ORIGIN      
        1 tcgtatttct tttagcgtca ccacacaaca ggtattgttg tgaaactcat gtttgcctct
       61 tcgggatttc ccatattcac cataactgaa aaagccgata aaaggagcat tccagacacc
      121 catcactctt tcaatttctt cacttgtcat tacaccaaaa gaaagataac ggctgatgca
      181 ggaaaacata atgaccgcat caacaccgtt cctgttgcct gttttcaaat ccgaacattc
      241 gttgacaacc gtatctataa catcaaaatc gggcggcagg gagaagcgga ctttggaacc
      301 ttgcggtaca ttccctgcac agatcaaaga cctgtcttcc ttattcccca acatggctgt
      361 tcgcatcacc ggcggtgcat cttctctttc cagttgtaac gggtaatagg cgccaagatt
      421 aaaaacggta ttggtaatgg cattcatatc atccatattc aacccgaggt atttgatcac
      481 aagatccagg gccggctgat catccatggt gtacaccaca tttccctcac ttttcgtcac
      541 cgttttagta atgccgattg gtttccatcc gcaggtggct attcccgata cctctaccct
      601 gtccgtatct ataataatgg ccacaaggcc ggtgatactg ctttttccct gtgtaaatac
      661 gattggtccg ctcaatgcaa ggtcatcgcc ggccatgccg ccataaatgg ctggttctcc
      721 ctggtaacct tcttcaattc ctttgatgat ccgttcgcca tcggtatgca accagccgga
      781 agcaaccaaa aatgcggctt gggcaaaact gtttttaccc aatacaccca attgttttga
      841 aacttcataa gctgaactgc cgcccgtttc caaaaacact aattggaaaa gagcaggatc
      901 gggatcaagc agcataatgg ctattccgcc ttcactgatc tcgctgctga tgaattcgcc
      961 ggatgtagtg gaaccgaaga tctgtatccc ctgttcattc agtaaggaac atacggcttc
     1021 ccggtcctgt ttcaccgaca tgaatacaat ggccaatgtt ggtttgaaat cgtttgttat
     1081 gcattgttgt aacgccgatt gaatttcccc ggttgaattt cccttgattg atttggcttg
     1141 catgtttaat tattcaactg ctccgaaaac gatggtaagc ccacccgttg cgaaattggc
     1201 cagatcggca gccatggctt gcccctccgg ggaacccaat gcctgctgca tttctgccgc
     1261 attgtcaaaa taaagttctg ccatgcggta ataagccgga gccgacccat caggattggg
     1321 caaaaatttc gtcagttcca atttaagcac accctgggtt tttgctacaa gcggcaagtg
     1381 cttttctaca taatacttct caaatgctgc agcgtccttg ggatggccat acagtactgt
     1441 tccttttatc atatcgattg attttatttt tcttttaaaa ccacccagct gttggtggtg
     1501 gaatgaaatt cgtgtttgcc attgggcccc ttcccaaatt cgccataggt gtaaaacccc
     1561 gccatcggaa cattccagac cccggccagc ttttcatttt catcccttgc caggggtccc
     1621 agagcgctca accttcccgc gcaggaaaag ataagcaggg catcggcctt cgcatgggtt
     1681 tgcattttta attcattggc tttccccact acggtttcga ggatgtcaaa atcaggcggc
     1741 gttgaaaacc ggaacctgga cccttcccgg atatcggact cgcagatcag ggccccttct
     1801 tccttattat agccgatggg attgcacatc atgggctcgc gaccctccct ttcgatctgg
     1861 aaagggtaat gcaccccgat ggtatcaaaa aaccggacct ggtcttctgc cgaagacctt
     1921 tccataccca agaagcggag gtacatgtcg agggcgggct ggccatcaat ggtataaagc
     1981 aggttgcccc tgcttttggt gatggtcctt gcaaccccca cgggcttcca tccggaaata
     2041 gccattccgc tcaggcttac tttctcctcg ttcaggatga gggcgaccag tccataatcg
     2101 gtggactgat catgggtaaa cacataggtg cccgtaaaac tgatgtcatc cccggccatg
     2161 cctccgaata gggtgacctg ggggccggtc actttttcta tactccggat gagggtctcc
     2221 ccctccaaca ttttgccatc cgccgacagc agggtgctga gcaggataaa agccggacag
     2281 tcaaaggttt gcaaggctct ccgtgccagg ttcatggcgg catcctccat ggtccgctcc
     2341 ccgatcggct cataaaatat ggcataatct gctttgggca attccagcaa caatacggaa
     2401 atcccccctt cgctttgtcc cgcctcggta aattcgccgg aagaggtggc ccccaggacc
     2461 tcaaggcctt tccggtgcag taaatcccgc aaagtaaccc agtcctgttt gatggagacg
     2521 aaaacaaagg caagttctgg cctgaactcc tgatccccag acctggctgt aagatccaat
     2581 atgtcttcca gcgcgtaccg gacctcttcc aatgaatttc cctggatcga tttcgccttc
     2641 attccccctg tataataatt cccggcatct ctacagtctg ttctcaaagc tacctaaatt
     2701 aatccggcgg agaggcaaaa gacaggaggt gttttgataa gcccttcggt ctttaatttc
     2761 atccgaccgg taaccggata ataaactcac tccctgatcc atcctctttc tccatcccca
     2821 actcccccga aggcgggaaa taattactga ttactttaat atccccaccg tgcgctttga
     2881 cgatatcata agccagggac aggcccagcc cagtggcttg cccggtcgtc ccgatgacta
     2941 ttgggagggt ggtaaaaaaa ggttgcgcca aaggcatccc ttcgggaaaa tattttctcc
     3001 cggatctgtt ccggaatacc gcttccattt tttttcactt taatttcaac ccccttttta
     3061 gttcagatat agtctcgaaa atctcgcaaa gtaacgttca aatcaggcga acagctaaaa
     3121 ccagaggcgt tttgaaaaca tccctggtct ttgcccatcc gacttcatca gacgggcaag
     3181 tgaataataa actcagtgta ggatccggga tccgtctcca cccccaactc ctccgaaggt
     3241 gggaaataat tgctgattac tttaatatct ccaccatgcg ctttgacgat atcataaggc
     3301 agggaaaggc ccagccccgt gccttgcccg gtcggtttgg tagtgaaaaa cggctggaaa
     3361 attttctccc ggacctgttc cggaattcct ggtccattgt ctttaaccct gatttcaact
     3421 cctttttcat ttcgtcttgt actgatgcgc accaaaggtt cataaccgga tatgttttgt
     3481 tttgcccttt cgtttactgc agccgtacct aatctttttc ttttctcatt tacggcgtag
     3541 aaggcattgt tgatcagatt gagtaaaacc cggctcatat cctgtggcac caggtctatc
     3601 cggggcagtt ccggatccgg atccaattca tattttgcct tgaaatccgg gtcttttgcc
     3661 cttaaaccat gataagaaag acggaggtat tcatcgcata aggtgttcag atcggtgggt
     3721 tcttttttgc tgctgctggt ccgtgaatgt tggagcatgg atttcactat ggcgtcggct
     3781 cgttgtccgt gttgatgtat tttttgttcg ttttctttaa gggtctgaag gagttgcgtt
     3841 tccagttgct cattcctgtc ttctttctgc tttgtccttt ctccttcaat ttcgccgatc
     3901 aactcctgat tgacctcggc aaaattattg acgaaattga gcgggttctg gatttcatgg
     3961 gcaatgccgg cggtaagttc acccagcgag gccattttct cggattgaat gagtttagac
     4021 tgggtggcct tgagggcctg atgggaagat tccagttctc tgtaagcttt ttctatgagc
     4081 ctggcttgtt cgagttcctt gagttgagtc ctgtcccttt ccctttttaa tacccgctgt
     4141 ttaaaatacc ggttggcgac cgaagccatt cccgcaaggc ccaccaggta taaggcgtat
     4201 gcccaataag tcctccacca gggataaaga atttgtatat tcagttttat gccctgttca
     4261 ttccagatcc cgttgctgtt ggccgcctta accctgaaga catattttcc gggagggaga
     4321 ttggtaaaat aagcggcctg ttggctgccc acgtcgatcc atcggtcgtc ataattttcc
     4381 agaatataag aaaaccggtt ttttgctgga tttgaataat gccccccgag gtattcaatc
     4441 gtgatattgt tttcatcatg atttagggtc agggaagcat cgtccgttaa acccttttca
     4501 atacgggtac cccagcctaa agccaaagtc cagatggaaa cctttacgtc tccgaatgaa
     4561 atcaaaggcg gtaccgctgt caatgatatc tcatccggat tgaactggat caattcttca
     4621 ccgctgaaaa aaaaacggcc atcggtagtc ttcaatgccg tgtaataacg ttttgaatcc
     4681 aatccgtctt ccgtcgaaaa gttggtaaat ttctccgact gcaaatcgaa acggctgatc
     4741 ccttgtcctg tacccagcca caaggccctg tttttttcat cgtggaggat ggcatataca
     4801 gaaatatcgg ccaatccatc ctcggtcgta tacgttttta tttttttatc ccgggaatta
     4861 tacctgttta acccccctcg ttgcatgccc acccaaacga tatccgaact gtcttcctgc
     4921 actaccgcat agatgctttg attcaaaaac acatcgccgg tttttttatc atagccatgc
     4981 cgggtgattg aatccgtatg gaaatcatac aggtagagcc cgttatccgt taacagccaa
     5041 aggccctttg acttgccgtc ttccacgcgc accatattgc ttctttcagt tcctcccggt
     5101 tgcctgctga ggtccatggg cacaaattcc tccgagcctt tcggtttttt atataaataa
     5161 ctgagcgtgc ccagccactc attgccaaag gcatcggtat aaaactggag gataccggga
     5221 tggatattcg gaaccccttt caatgaaact ttttgcgctc ttttattatc caggtttaag
     5281 cggtacaccc cgtggtctgt cccgaatgtt aaggtatccg tcccggttga atggacaccg
     5341 aaaaccatgg atcccttggg gaaaatggat ccgatgtggt gttttttaat ggtattggtt
     5401 tgtaaatcca gttcgttcag gaccgggatg gaagtaccga cttcataaga aagtaccaga
     5461 atttttgctt tcccggcctg agtcaaagac acggcatatc ccttcagggt ggaggccgtg
     5521 tcgtcgttct ttgaagtata gttaataaat accggcttat cttcatgttt cagcaagcca
     5581 ttttgtgtac cgatccaaag gttattaaag gaatcaagat tcaggctccg cacttcataa
     5641 ttttttccgc ggtgatcttt gggcaactta aaaaattcag atttttcatt tttaaggtca
     5701 aaacggatca atcccttgtc caggccgatc cacagattct tattttgatc caatttcaga
     5761 atacaggact ggaggggttt ggagggatcc ggtaaaaggt gccggaaaga atacgttttt
     5821 aacggagccc catccagctt gtcaaagacg gcgaggctgc cctggtcaga cataaaccaa
     5881 agccggtcct cctggtcaaa aatgaaatcc agtacgctgg tttgattggc caggttctga
     5941 agctccgtgt aggggccaag cctggacaat gattttcctt ctttaaaaaa aaacaattgg
     6001 ttatccaaac ccaagaacag ggtatttttg gcatgatccg gtcttgccag ataaaacccg
     6061 cctggattgg cactgtcggg taagggaaaa ggccttatcc ggtccccgga ttcatcataa
     6121 atcaataacg aaacaggaag cggggatacg ctgttggccc caaaatataa acggcccccg
     6181 gcatccatgg atatggtgga tatgccaaaa tcgttgtttt ccatggtttt accgatcaat
     6241 tgaaaaaagg gataggtttt aaaggaaccg gagcgcgggt cgaaacgata aagccgctca
     6301 tcaaaagcgc tgatccatat attgcccagc gcgtcctcgt acaatgttga gatcaccgct
     6361 cctttgatgg atgaggaatc cttcgggtca tatacaaatc ttttaaattc atacccttcg
     6421 aacctcatga gtccatcact ggtgcccagc cagattcgcc caaattgatc ggaaagaacc
     6481 tcccaaatct ccaccccctg ggtttcctta taataggtga aattcagttc cttttggccc
     6541 gggacggccg tgttcaggag gaaaataaaa gccaaagcca cacaaatccg taaagccatt
     6601 ccggtgcagg gtatgtataa ttaataaaga aacaagataa taaaattggg ggtaggatta
     6661 ataggattaa taggattggt aggattaata ggattggtag gattaatagg attggtagga
     6721 ttggtaggat taataggatt ggt
//
LOCUS       DAOQXT010000172         6491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_12281_length_6491_cov_5.196240, whole genome shotgun sequence.
ACCESSION   DAOQXT010000172 DAOQXT010000000
VERSION     DAOQXT010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12281_length_6491_cov_5.196240"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKM27_18540"
     CDS             <1..570
                     /locus_tag="PKM27_18540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNR09325.1"
                     /translation="IEPLLIRKPFNRFDFFIAFLIVPGMALVVQNIDAERYGGVLAGL
                     FSAFLAAVFSSLNKKYITRASVWNISWIEMTSALLTLSILLWIYQAMAGPLPWWPAKA
                     DWAYLLILGVLCTTIAHAMSLFALHHVSAFALNLVINLEPVYGILLAMVLLKENKSLN
                     AGFYTGAAVILIAVLSYPYLQKRLSHVAR"
     gene            557..1204
                     /locus_tag="PKM27_18545"
     CDS             557..1204
                     /locus_tag="PKM27_18545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNR09326.1"
                     /translation="MWPDERILDYCDRYSFELPDYLNQLERETGLSTVNPFMSCGPVM
                     GRFLIQLSTWLKPKSILEIGSFTGYSALCLAQGLDEEGRITCIEVNEEYEEVIRKYFH
                     LADKSRQLDLVIGDARSRLQDLHGPFDLVWIDANKQFNEELYESVLPLLSRAGTILVD
                     NVLWYGNVLDSRPDRETRAIQAFNEKIKSDPRVKAMILPLRDGITIIQKVSLAAS"
     gene            complement(1205..2962)
                     /locus_tag="PKM27_18550"
     CDS             complement(1205..2962)
                     /locus_tag="PKM27_18550"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNR09327.1"
                     /translation="MKNGSSFILFSLLAVNLFGQDPAKLALWLDDHPVISIHRTKDEA
                     ANRLLQVIDSFQTQGYLACHYEVLAESDTLLEVRIQAGSKYVWAKVDMSGIPLPFLIN
                     SGILRRKDVDQDWNLDQCRSVMQQLAAHLAEEGYPYAVVFLDSSRLEEQKINARMNLV
                     TGPRVTLDTLEWDDGLKISPSLLLRILGIQSGSPFNKKKIDQGVQALAAYSFVEPYAD
                     PDITLSRGLARVRLYLRTRRTSQVDALIGILPSSERGGKLKFNGNFRADLLNQLGRGE
                     RFTFDFQSLQSSSQELRIRAGAPYLFQLPFHPSFQFNLYRRDSLFLDVFAELGATLPV
                     GERGEIRFFIGQKSSSLLSPDLRQILISHRLPDALDLRLNHLGAALRFRRLDFERNPR
                     RGYSFSLYAHGASRKIIPNSSITSLRDPADSSFSFAGLYDQVKKNGYAFDVQVEAEKY
                     TVLGRYGTWHSWLRAASRQASGLLQLNERWRLGGFKLLRGFDEESIFASAYSMWTNEA
                     RLLTGEWSYIQVFSDLALVNQYTGEQYQNAVFWGLGAGLTFNTSLGNLSVSAAAGRRF
                     PASFDLRGIKLHIGYLTVF"
     gene            2961..4127
                     /gene="hemW"
                     /locus_tag="PKM27_18555"
     CDS             2961..4127
                     /gene="hemW"
                     /locus_tag="PKM27_18555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNR09328.1"
                     /translation="MTNSPRKYRKPVFCPFTGMAGLYIHIPFCKQACTYCNFHFSTNL
                     KYTDELVTALHRELAQRKTFLPGRRISTIYFGGGTPSLLRAGQIKSLISDIQAWYEVD
                     PEPEVTLEANPDDLNPLYLHQVREAGINRLSIGVQSFFDEDLKFMRRAHHSRQALDSI
                     RMSREAGFENITIDLIYGIPGSSMDQWRQNLFQAFELEVPHLSCYALTVEPRTILSDW
                     IKKGKAAVPEEEETIRQFEFLMEEAERRGWRHYEISNFAKAGFESRHNSSYWEGTEYL
                     GIGPSAHSYREGERQWNVANNHLYLKALAEGLPHYESEQLSRENIYNEYILTRIRTAA
                     GLDLFSVPESFRAHLVRCADKHLQQGHLEQEGNFYRLTRKGKFIADRVTVDLFL"
     gene            complement(4147..6192)
                     /gene="scpA"
                     /locus_tag="PKM27_18560"
     CDS             complement(4147..6192)
                     /gene="scpA"
                     /locus_tag="PKM27_18560"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNR09329.1"
                     /translation="MKGKGIRFREVPFEPSMKGTPVPPVTGFPPFRGGPYATMYVSQP
                     WTIRQYAGFSTAEESNAFYKKNLAAGQRGLSVAFDLATHRGYDSDHPRVRGDVGKAGV
                     AVDSVEDMKILFDGIPLHKMSVSMTMNGAVIPILAFFIVCAEEQGVPSEQLTGTIQND
                     ILKEFLVRNTYIYPPAASMRLVTDIFKYSSARLPRFNSISVSGYHMLEAGAPPPLELA
                     FTLADGYEYLQYGIKAGLHIDEFAPRFSFFFGIGMDGLREIAKLRAARILWARLVKAA
                     GSEAPRSQVLRTHCQTSGWSLTQQDPFNNIARTALEALAATWGGTQSLHTNSLDEAVS
                     LPTAATSAIARETQLFLQKEAGLCHWPDPVGGSIHIESLTDEMVDESWKIIKEVLSLG
                     GMTRAIQDGYPKKRIEEAAALKQARIDSGEEKIIGLNLYPPDQELEIPVLQIDHEEVL
                     QIQLKKLETLKKNRSQSRVRQALQHLSKACKDEKENILERAIEAARNRATLGEISSAI
                     EKVFGRYHAPAGGLSKIYAAHSMNNRYFKKACKLVLDFEKKFGRRPRILIAKMGQDGH
                     DRGARIIASGFADLGFDVDMGPLFQTPAETARHAMENDVHFIGVSSLAGAHQALIPEL
                     IGLLKEKGRDDIAVIAGGIIPDTDARALFDKGVLAVFGPGTNLAKAAVELMKLSLRG"
     gene            complement(6189..>6491)
                     /locus_tag="PKM27_18565"
     CDS             complement(6189..>6491)
                     /locus_tag="PKM27_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09330.1"
                     /translation="HDLMKDALGFIPWSDPASYTSMYRWMVFLLISLAGWMVYLYKKN
                     PHPFVLLGLLKLSLVVFYHFIPIPYTYLFHTSVWVSMAIFFVYSGHREENVYNFTE"
BASE COUNT         1558 a         1723 c         1694 g         1516 t
ORIGIN      
        1 atcgagcccc tcctgatcag aaaaccattc aaccgctttg atttttttat cgcctttttg
       61 attgtccccg gaatggctct cgtcgttcaa aacatcgatg ccgaaaggta cggcggggtg
      121 ctcgcgggac ttttctccgc gttcctggcg gctgtttttt cgagcctgaa taaaaaatac
      181 atcacgcggg ccagcgtttg gaatatttcc tggatagaaa tgaccagcgc cttgctcacc
      241 ctcagtatcc tcctttggat ttaccaggcc atggcagggc cccttccctg gtggcccgcc
      301 aaagcggatt gggcctatct cttgatcctg ggtgtgctat gcaccaccat tgcccatgcc
      361 atgtcccttt ttgccttaca tcatgtttct gcctttgccc tcaacctggt catcaatctc
      421 gaaccggtgt acggcatctt gcttgcgatg gtcctcctga aagaaaataa atcactgaat
      481 gccggttttt ataccggtgc cgcagtgatc ctgatagccg tactgagtta cccctacctg
      541 caaaaaagat tgtcccatgt ggcccgatga acgcatcctg gattattgcg accggtacag
      601 tttcgaactt ccggactacc tcaaccaact cgagcgggaa accggacttt ccacagtcaa
      661 cccctttatg tcctgcggtc ccgtcatggg ccggtttttg atccagctca gcacctggtt
      721 gaaacccaaa agcatactgg aaataggcag ctttacgggt tattcggcgc tctgtctggc
      781 ccaggggctt gacgaagagg gccggatcac ctgcatcgaa gtaaacgaag aatatgagga
      841 ggtcatccgg aaatattttc atctcgcgga caaaagccgg cagctggacc tggtcatcgg
      901 ggatgcccgt tcccgcctcc aggatttaca tgggcccttc gatctggtat ggatcgacgc
      961 caacaaacaa tttaacgaag aactttacga gagcgtgctg ccgttgctca gcagggccgg
     1021 gaccattttg gtggacaacg ttctctggta tggaaatgta ttggatagcc ggcccgaccg
     1081 ggaaaccagg gccatccaag cttttaatga aaaaataaaa tccgatccgc gggtaaaggc
     1141 gatgatcctg cccctgagag acggaattac gattatccag aaggtaagcc tcgccgcaag
     1201 ctgatcaaaa aaccgtgagg taaccaatat gcaatttaat tccccggagg tcaaaggaag
     1261 ccggaaaccg ccggcccgcc gcagcggata ccgataaatt acccaatgaa gtattgaagg
     1321 tcaaacccgc tcccaggccc cagaataccg cgttctgata ttgttctccg gtatattgat
     1381 tgaccagggc caggtcggaa aatacctgga tataagacca ttccccggtg aggagccgcg
     1441 cctcattggt ccacatggaa taagcgctgg caaaaatgga ttcttcatcg aaacccctaa
     1501 gcaatttaaa accgcccaat ctccatcttt cattaagctg cagtaaaccg gatgcctggc
     1561 ggctggccgc gcgcagccag ctgtgccagg tgccatacct gccgaggacc gtgtattttt
     1621 ccgcctccac ctgaacatcg aaggcatacc catttttttt gacctggtca tacaagccgg
     1681 caaaagaaaa actggaatcc gccgggtccc tgaggctcgt aatggacgaa ttaggaatga
     1741 ttttgcggct tgcgccatgg gcatacagcg aaaaggaata tccccttctt ggattccttt
     1801 caaaatccag tcgcctgaat cgaagggcgg caccgagatg attcaagcgc aagtccaggg
     1861 catccggcaa acgatggctg atgaggattt gccgcaaatc gggtgacaac aaagaggagg
     1921 atttctgccc gataaaaaac cggatttctc cccgttcccc gaccgggagt gtcgcgccca
     1981 gttcggcgaa gacatccagg aacagggaat cgcgacggta gaggttaaat tgaaaagagg
     2041 gatggaacgg aagttggaag agataaggtg cgcctgccct gatcctcaat tcctggctgc
     2101 tggattgcag gctctgaaag tcaaaggtga accgctcgcc ccttccgagt tgattcagca
     2161 ggtccgccct gaaattacca ttgaatttta atttccctcc gcgttcgctg gacggcaata
     2221 taccgatcag ggcatccacc tggctggtgc ggcgcgtccg gaggtaaagc ctcacccggg
     2281 caagacccct cgacagggtt atatccggat cggcataggg ctccacgaag gaataagccg
     2341 ccagggcctg caccccttgg tctatttttt tcttattgaa tggagaaccg ctctgaatgc
     2401 caaggatgcg taacaggagg gaaggggata ttttcaatcc atcgtcccat tccagggtat
     2461 ccagggtcac cctaggtccg gtgaccaggt tcattctggc attgatcttc tgttcctcca
     2521 aacggctgct gtccagaaaa accaccgcgt agggatatcc ctcctctgca aggtgagccg
     2581 ccaattgttg catgacagac cggcattggt ccaggttcca gtcctggtcc acatccttgc
     2641 gccggagtat acccgaattg attaaaaaag gaagtggaat gccgctcatg tccactttgg
     2701 cccagacgta ttttgaaccg gcctggatgc ggacctcgag cagggtatcg gattcagcca
     2761 ggacctcata atggcaggcc aggtatccct gggtctgaaa agagtcgatc acctgcagga
     2821 gcctgttcgc agcctcgtct ttggttctat gaatggaaat cacggggtga tcatccagcc
     2881 acagagcgag cttcgccggg tcctgtccaa acaggtttac cgccaagaga ctgaaaagaa
     2941 tgaacgatga cccatttttc atgaccaatt ccccgcgaaa atacaggaag cctgtatttt
     3001 gcccttttac cggcatggca ggcctctata tccatattcc tttttgcaaa caggcatgca
     3061 cctattgtaa ttttcatttt tctaccaatc tcaaatacac cgatgaattg gtcacagccc
     3121 tgcacaggga acttgcccag cgtaaaacct ttttgcccgg aaggcgtatt tcgaccattt
     3181 attttggcgg gggtacccct tcccttctcc gcgccggtca aataaaatcg ttgatctcag
     3241 atatccaggc atggtatgag gtagaccctg aacccgaagt gacccttgag gccaacccgg
     3301 atgatctgaa tcctctttat ctccatcagg tccgggaggc ggggatcaac cggctgagta
     3361 tcggcgtcca atcctttttc gatgaggacc tcaaattcat gcgccgcgcg catcattccc
     3421 ggcaagcgct ggattccatc cgcatgagca gggaggcggg attcgaaaac atcactattg
     3481 acctgattta cggcataccc ggctcgtcca tggaccaatg gaggcagaac cttttccagg
     3541 cttttgagct ggaggtgccg catttgtcct gttacgcact cacggtagaa ccccggacca
     3601 tcctttcgga ttggataaaa aaagggaaag ccgcagtccc ggaggaggaa gaaaccatcc
     3661 ggcaatttga attcctcatg gaagaagccg aacgccgggg ctggaggcat tatgaaattt
     3721 caaattttgc caaggccggt tttgaatccc ggcacaacag cagttattgg gagggcactg
     3781 aatacctggg catcgggcca tccgctcatt cctatcgcga aggagaacgt caatggaacg
     3841 tagccaacaa ccatctgtac ctgaaagccc tggctgaagg acttcctcat tacgaatcgg
     3901 aacaactcag ccgtgaaaac atctacaacg agtacatttt aacccgcatc cgcacagctg
     3961 caggactgga tcttttcagc gtgcctgaat cgttcagggc ccacctggtc cgatgcgcgg
     4021 acaaacatct tcagcaagga cacctggagc aggaaggaaa tttttaccgg ctgacccgga
     4081 aaggaaagtt catagcggac cgggtaacgg tggatttgtt tctgtgaaaa aagaggcctc
     4141 ttcggttcat cctctgagac ttagtttcat caattcgaca gccgctttgg ccagattggt
     4201 cccggggccg aagacggcga gcactccttt gtcaaacagg gcccgggcat cggtatccgg
     4261 aataataccg cctgcgatca cggcgatatc gtcccggcct ttttccttaa gcaatccgat
     4321 cagctccgga atgagggcct gatgggctcc ggccagggaa gataccccga tgaaatggac
     4381 atcgttttcc atggcatgcc tggccgtctc ggccggggtt tggaagaggg ggcccatgtc
     4441 gacatcaaaa ccaaggtcgg cgaaaccgct ggcaatgatg cgggcaccgc ggtcatggcc
     4501 atcctggccc atttttgcga tcaggatcct tggccttctt ccgaattttt tctcaaaatc
     4561 caggaccagc ttgcacgctt ttttgaaata tctgttgttc atgctgtgtg ctgcatatat
     4621 tttggacaat ccccccgcag gggcgtgata ccggccgaag accttttcaa tagccgaaga
     4681 aatctctcca agggtagccc tgttccgggc cgcttcgatg gcccgttcca gaatgttttc
     4741 tttttcatcc ttacaggctt ttgacaggtg ctggagtgcc tggcgcaccc tgctttgact
     4801 gcggtttttt ttcagggttt ccagcttctt taactggatt tgcaatactt cctcatgatc
     4861 gatctggagg acgggaattt caagttcctg atcaggagga taaagattga gtccaatgat
     4921 tttctcttcc ccgctgtcta tccgggcttg tttcaatgcg gcagcttctt caattctttt
     4981 tttgggatat ccgtcctgta tggccctcgt catcccaccc agcgaaagga cctctttgat
     5041 gattttccaa ctctcgtcca ccatttcatc ggtcagggat tcaatatgga tggaaccacc
     5101 gaccggatcg ggccaatggc agagtcctgc ttccttctgc agaaaaagtt gggtttcccg
     5161 ggcgatcgcc gaggtggctg cagtcggaag ggataccgct tcatccaggg aattggtgtg
     5221 cagggattgt gtgccgcccc aggttgccgc aagggcttcc agggcggtcc gtgcaatatt
     5281 attgaaaggg tcctgctggg tcagcgacca gccggaagtc tggcaatggg tgcgaaggac
     5341 ctgggacctt ggagcttcgc tgccggcggc ttttacgagc cgggcccaga gaatacgggc
     5401 tgcccgcagc ttggcgattt cacgaagacc atccatgccg atgccgaaaa agaaagaaaa
     5461 acgcggcgca aattcatcaa tgtggagacc ggctttgatc ccgtattgaa ggtattcgta
     5521 tccgtctgcc agggtaaatg ccagttcaag cggaggggga gcgccggctt cgagcatatg
     5581 gtagccgctc acgctgatgg aattgaaccg gggaaggcgg gcagaggaat atttgaagat
     5641 atcggtcacc agcctcatgg aggccgcagg gggataaatg taggtattgc ggaccaggaa
     5701 ttctttcagg atatcatttt ggatcgtgcc cgtaagttgt tcactgggca ccccctgttc
     5761 ttctgcgcag acaataaaaa aagcaaggat gggtatgacg gccccgttca tggtcatgga
     5821 cacggacatc ttatgcagag gaatcccgtc aaacaggatt ttcatatcct ccacgctgtc
     5881 cacggcgacc ccggcttttc ccacatcgcc cctgaccctg ggatgatccg aatcataccc
     5941 ccggtgggta gccaggtcaa aggccacgct caatccccgc tggcctgcag cgagattttt
     6001 tttgtaaaag gcattgcttt cttcagcagt ggagaagccc gcgtattgcc ggatggtcca
     6061 gggttgtgaa acatacatgg tcgcgtaagg tccgcctcgg aatggaggga aaccggtcac
     6121 cggaggcacc ggggtccctt tcatggaggg ttcaaaaggg acttcgcgaa atcggatacc
     6181 cttgcctttc attccgtaaa attatacaca ttctcttccc ggtggccgct atacacaaag
     6241 aagatagcca tggaaaccca cacagaggta tgaaaaaggt aggtataggg tatgggaata
     6301 aaatgataaa acaccaccag gctgagtttg agcaggccca ggaggacaaa cgggtgcgga
     6361 ttttttttat acaggtacac catccagccg gcgagggaga tcaataaaaa gaccatccac
     6421 cggtacatgg atgtatagga agccgggtcg gaccaaggga taaatcccag tgcatctttc
     6481 atgagatcat g
//
LOCUS       DAOQXT010000173         6448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_12384_length_6448_cov_7.006257, whole genome shotgun sequence.
ACCESSION   DAOQXT010000173 DAOQXT010000000
VERSION     DAOQXT010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12384_length_6448_cov_7.006257"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..1277)
                     /locus_tag="PKM27_18570"
     CDS             complement(354..1277)
                     /locus_tag="PKM27_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09331.1"
                     /translation="MALWIAGSCSLKHGRTLDPSGPEMASSFKISMGDLTAGFVDNSA
                     IPPFHRAGYNGLASLQHRLDDSTFFVPQYAGFNLEHIFGGDSLISLFELRRHPMTLYR
                     KSDSEVLLYQTPTPISRVESLTAFKLVPPHYIDIRFTCQLNDLSFFRHGYAGLFWASY
                     IQAPEDKKIYFLSTGSLSKGESWINTYSETHGVNSTHRGRKDDHQLFFAGNFNASLAS
                     HFSRFRFVQPFYFGLCRKMVFAQLFDSKEIIRFSQSPTGGGPANPAWDFQYIIPEPKA
                     GKRYSFKARLIYKPFVSAEDIRLEYVKWKAG"
     gene            1604..3025
                     /locus_tag="PKM27_18575"
     CDS             1604..3025
                     /locus_tag="PKM27_18575"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR09332.1"
                     /translation="MKNILLILVLTVAAGYRLVAQNPWSLERCVNHARDNSIAIKQSR
                     LGIERAQVSAEQALHNRYPRLNASLNYGLNFGRTIDPVTNDFETQSITTNSLGLNTGM
                     VLFNGGSVRNTISQSRINVQSATLDAQQFSDDLALNVVQYYLAILFAEDRLVNARLQL
                     ETTTQQLDQMDRMIAVGTRPEGDRLEIVAQQARDEQSIATIENTVNTNKLLLKQLLRL
                     TIDEPFDIARPPDDALQPSIISENLVAVYNHALNNRADIRSGDFKVQSAEYGIKLAKA
                     NYLPTISIGGNLNSLFSSLGRRITGYNDINVPQEIIFNGQSATIEIPTTVPLTGKNPY
                     FSQLNENIGYGVGLGLSIPIYNNYSAKAQVRQAEIAVEAAKLEADQTKETLKNNIQTA
                     LNELRATANTYEVSKRATAASRLAYDNTKIKFDLGSANTFELLSSKNRLDIAVTEELL
                     SRYDYIFRSKVVDYYLGRPIRLN"
     gene            3071..4480
                     /locus_tag="PKM27_18580"
     CDS             3071..4480
                     /locus_tag="PKM27_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR09333.1"
                     /translation="MEPRTSKGKKKGNQWLIWALLALFVALMAFAYFKNKKKPKGEKV
                     TTEKVGRRTIRETVSASGKIFPEKEIKISSDVSGEIVELEVREGDSIRAGQLLARINP
                     DTYESAVERGNASLNNARSQVSISQAQVDAAKAQIEQIEANLANATKIHNRNIELRKQ
                     GVISQADFDASLANVHALEANLKSAKSNLNSSIQGTRAAEFTVKSAEAGLKELRTSLS
                     RTSIYAPSSGIISKLNVEEGERVVGTIQMAGTEMMRIANLNQMEVQVDVSENDILRVR
                     LGDTAEIEVDAYLDRVFKGYVTEIANSASNTGTTTIQTLSSDQVTNFVVKIRILEETY
                     ADMMTGGRFPFRPGMSAAVEIFTNTKEDILCVPIAAVTTREDENKDSLVARQRAFEFK
                     EYVFVYSSDTAKMVRVRSGIQDDNYIEVLEGLEGGEEVITGPYATVSRKLKSGMKLQR
                     EEKKEESKKNGASVSVTVD"
     gene            4561..5166
                     /locus_tag="PKM27_18585"
     CDS             4561..5166
                     /locus_tag="PKM27_18585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNR09334.1"
                     /translation="MEQALIIFIKNPVAGKVKTRIASESGDSAALDIYKSLMEHTRLM
                     SLKVDATRMLFYSDRVERNDSWPEKKFSKNVQLGEDLGTRMLNAVKQASEYDKKILIG
                     SDCPGITPQIIEQAYTYLDFHDVVIGPTYDGGYYLIGMNQIVPELFQHIPWSTDQVLA
                     ETIKVLQQKRLLYKMLPTLRDIDTLEDWEAEKNKLVREQTI"
BASE COUNT         1721 a         1696 c         1574 g         1457 t
ORIGIN      
        1 ctagcgccag cccgcgcggc agctgctggc agccgccctt ggctttcacc tggcgtccgc
       61 ccgcgcggaa gcgggtggaa gccgccttgg ctttcaccta aggctagccc ctgcggaaga
      121 aggtggaagc caattaaact ttcccctagc gccagcccgc gctgaagctg ctggcagccg
      181 ccttggcttt cacctagcgc cagcccgtgt ggaagcgggt ggaagccaat taaacctcac
      241 ctggcgctag ctcgtgcgac agcgggtgga agctaccttg gctttcacct agcgtcagcc
      301 cgtgcggaag cgggtggaag ccaattaaac tttcaagtga tttcagtacc ccattaccca
      361 gccttccatt ttacatattc caaccggata tcttccgcgg atacgaatgg tttgtagatc
      421 agcctggctt taaaagaata gcgtttcccg gcctttggct ccgggatgat gtactgaaag
      481 tcccaggccg gattggctgg accgcccccg gtaggagact gggaaaaccg gatgatttct
      541 tttgaatcaa agagttgggc aaaaaccatt ttcctgcaca ggccaaaata aaacggttga
      601 acaaaccgga atctggaaaa atggctggcc agggaagcgt taaaattccc tgcaaaaaaa
      661 agttggtggt cgtccttccg gccccggtgc gtgctgttta ccccatgcgt ttccgaatag
      721 gtgttgatcc agctttcacc cttggaaagg ctgcctgtgg acaggaaata tattttttta
      781 tcctccggtg cctgtatgta tgatgcccag aacaatccgg catagccatg cctgaaaaag
      841 gataggtcat tcagctggca ggtaaaacga atatcaatat aatgaggagg caccagctta
      901 aatgcggtga gcgattccac cctggaaatg ggggtcggcg tttgatataa caaaacttcc
      961 gaatcacttt ttcggtacaa agtcatggga tgcctgcgta gttcgaacaa ggagataagg
     1021 ctgtcaccac caaaaatatg ctctaagttg aagcctgcat actggggcac aaagaaggtc
     1081 gaatcgtcca gccggtgctg cagggaggcc agtccgttgt aacccgccct gtgaaacgga
     1141 ggaattgccg aattatccac aaatccggca gtcagatctc ccatggaaat tttaaaactt
     1201 gaagccattt caggtcctga aggatccagg gtccggccgt gtttcaggct gcatgaacct
     1261 gcaatccaga gagccaaaag gcatatattc ctatgactct gaaagaaaca catattaaag
     1321 aaattttctg attgaagata aggtaccggc ctgaagtacc tggatttatt ttaaccatgg
     1381 ctccccgggc cagggaggct cccatccgaa gcctgaaatg ggcgtacttt tgcaccctgg
     1441 taagagggta aggtgaggga atcagccggc ctgctcccgg gtccaaaatg gatcgggtca
     1501 ggaacagccg gttcatcgat agtctggacg gtttaataga aaaaatgaaa caacttcctg
     1561 attttcatat aatcttgttt gttcaatttt aaaaagtttt ggcgtgaaaa atattctttt
     1621 aatcctggtt ctgaccgtgg ctgcaggata caggctggtg gctcaaaacc cctggtctct
     1681 ggagcgctgc gtcaatcatg cccgggacaa cagcattgcc atcaaacaat ccaggctcgg
     1741 tatcgagcgg gcccaggtga gcgctgaaca agccctgcat aaccggtatc cgcggttgaa
     1801 tgcctcactc aattatggtc tcaatttcgg ccgcaccatt gacccggtca ccaacgattt
     1861 tgaaacccag tccatcacga ccaacagcct gggtctcaat accggcatgg tcctgttcaa
     1921 cggcggttcg gtgagaaata ccatcagcca gtccagaatc aatgttcaga gcgccaccct
     1981 ggatgcccag caattttccg atgatctggc cctcaatgtg gtccagtatt acctggccat
     2041 tttgtttgcg gaagaccggc tggtcaatgc ccgccttcaa ctggaaacca ccacccagca
     2101 actcgaccag atggaccgga tgattgccgt aggcacccgg ccggagggag accgtttgga
     2161 aatcgtcgcc cagcaggcca gggatgaaca aagcattgcc accattgaaa acaccgtcaa
     2221 taccaataag cttttactca aacagctcct gcgattgacg attgacgaac cgtttgacat
     2281 cgcccgccca cccgatgatg ccctgcaacc ttccatcatt tcagaaaacc tggtcgctgt
     2341 gtacaatcac gcgctgaaca accgggcgga tatccgttcg ggggatttta aagtgcagag
     2401 tgccgaatat ggaatcaaac tggccaaggc taattatttg cctacaatct ccattggtgg
     2461 caacctgaat tccctgtttt caagcctggg gcggcgcatc accgggtaca acgacatcaa
     2521 tgtaccccag gaaatcatct tcaatggcca gtctgcgacc atagaaatcc cgaccacggt
     2581 acccctcacg ggaaagaacc cctatttctc ccaattgaat gaaaacatcg gatacggcgt
     2641 tggcctgggg ctgtccatcc cgatctataa caattattcg gccaaagccc aggtacgcca
     2701 ggctgaaatc gccgtggagg ccgccaagct cgaagccgac caaaccaaag aaaccctgaa
     2761 aaacaatatc cagacggctc tgaatgaact cagggccacc gccaatacct acgaagtatc
     2821 aaaaagggcc accgccgcct cccgcctggc ttatgacaat accaaaatca aatttgacct
     2881 gggctccgcc aacacttttg aattgctcag ctccaaaaac cgcctggaca tcgcagtcac
     2941 cgaagaactg ctttcccggt acgattatat tttcaggagt aaggtggtgg attattatct
     3001 tggaaggccc atccggctca attaatattg aaaatcattc actcaatcac atttaaacag
     3061 aagcaggaat atggaaccgc gtacctcaaa agggaagaaa aaaggaaacc aatggttgat
     3121 atgggcgctg ctggccttgt ttgtggcttt gatggccttt gcctatttta aaaataaaaa
     3181 gaaacccaag ggggagaagg tcaccactga aaaagtaggc cgccggacca tccgggaaac
     3241 cgtgtcggcc agcggtaaaa ttttccctga aaaggagatc aagatcagct cggacgtctc
     3301 cggtgaaatc gtggaactgg aagtaaggga aggggacagc atccgggcgg gccagttgct
     3361 ggccaggatc aatccggata cctatgaatc cgccgtggaa aggggaaacg cttccctcaa
     3421 taatgcccgc agccaggttt ccatcagcca ggcccaggtg gatgccgcca aggcccagat
     3481 cgaacagatc gaagccaacc tggccaacgc aacgaaaatt cataaccgga atattgaact
     3541 ccggaaacag ggagtgattt cccaggccga ttttgacgct tccctcgcca atgtccacgc
     3601 cctggaagcc aatctcaaat ccgccaaatc caacctcaac agttccatcc agggtacccg
     3661 ggccgctgaa tttacagtca agagcgccga agccgggctt aaggaattga ggacttccct
     3721 ttcccgcacc tcgatctatg ctccttcttc cgggatcatt tccaaactga acgtggagga
     3781 aggtgaaagg gtggtgggta ccatccagat ggcgggtacc gaaatgatgc gcatcgccaa
     3841 cctcaaccag atggaagtcc aggtggacgt ttccgaaaac gacatcctcc gggtaagact
     3901 gggcgacacc gctgaaatcg aagtggatgc ctacctggac cgcgtgttca aaggttatgt
     3961 cacagaaata gccaattccg cttccaatac cggaaccacc accatccaga ccctgagttc
     4021 cgaccaggtg accaattttg tggttaagat ccgcatcctg gaggaaacct atgccgacat
     4081 gatgaccggg gggcgctttc cattccggcc gggcatgtcc gccgccgtag aaatatttac
     4141 caacaccaag gaagacatcc tctgtgtgcc aatcgccgca gtgaccaccc gggaggatga
     4201 aaacaaagat tccctggttg cacgccaaag agccttcgaa ttcaaagaat acgtctttgt
     4261 ttacagctct gataccgcca aaatggtccg cgtgaggtcc ggcatacagg atgataatta
     4321 tattgaggtc ctggaggggc tggaaggggg tgaggaagtc atcaccggcc cctatgccac
     4381 tgtgagccgt aagctcaagt caggcatgaa acttcagcgt gaagaaaaga aagaggaatc
     4441 aaagaaaaac ggggccagcg tctctgtgac cgtagattag tctgaatccc tccctttttc
     4501 cttattgtct tatgcattaa ttttgcaaaa ttcaaggatc cacctcaagc atgaaggctt
     4561 atggaacaag cactcatcat tttcattaaa aacccggtcg cgggcaaggt caaaacacga
     4621 atagcttcag aatcagggga ttccgctgcc ctggacattt ataaaagcct gatggaacat
     4681 acccggctga tgagcctcaa ggtcgatgcc acccggatgc tcttttattc cgaccgggtg
     4741 gagcggaacg acagctggcc ggaaaaaaaa ttcagcaaaa acgtccaact tggggaagac
     4801 ctgggcaccc ggatgctgaa tgctgtcaaa caagcctccg aatacgataa aaaaatcctc
     4861 atcggcagcg actgcccggg catcaccccg caaatcatcg aacaggccta tacctacctg
     4921 gattttcacg atgtagtcat cgggccaacc tatgacggag gctattatct catcggcatg
     4981 aatcagatcg tcccggaact cttccagcac atcccctgga gcaccgacca ggtcctggcc
     5041 gaaaccatca aagtcctcca gcaaaaaagg ttgctgtata aaatgctgcc caccctccgg
     5101 gatattgata ccttggagga ctgggaagcc gagaagaaca agctggtccg ggagcagacg
     5161 atatagcgtc tccgcaccct ccaacctttc gcctgatttc acagaagaag gggtctgtat
     5221 taatggtacg cgcggagttt acagggtcga cggagaagtt tcttttcaaa atttattctt
     5281 ctgtgtcttt gtgggagata ttgagcttca cccaaacatg gggcaggctg tgtcgggaca
     5341 ctagatcctg tatggatttc gtacgggtag cataaaattt taagattaaa tatcgtcccg
     5401 gatgggacga aataacctta ttcgtatgac gtgcctaaag gctccatttg agatcgcgat
     5461 ttatacgaaa ctatatcatt atatgttttc tgagccgaag tccgaatgga gggatgggcc
     5521 gaagctaagc gtcgtcctaa catgggtgcc tgttgtgtcg ttgtgtgcaa catcgccgcg
     5581 tcgttgtgga aattaacccg taccctttgt gtcgttgtgg gaccatcgcc gcgtcgtcgt
     5641 ggggaaaact tttgtgccct tcgcttcgct gtgtcgttgt gggcaaccca tagtgccctt
     5701 gtgggaaacc tctcaagcat cgaagatgct cgacgttttt aatactcatt caaagtaaaa
     5761 ccccggcttc ggagaagccc aacattcgtc gaacatctac gatacaagac ctcaccgaac
     5821 ccctaccaaa acgatgacca ccccaaccga ctatatcgaa aaaaataatg gccttttaat
     5881 tgagacttcg attgttgagc ttctccgaag cccttgtttt gttcttattt tttaccaaaa
     5941 acgtcatgca tctccgatgc cggaagaaac cgggggaatc aaatccttgc tcataaatgc
     6001 acctagcctt gcatgaactc ttcaatgctc tttgtgacgt ggtgggaacc acttcctgtg
     6061 tgggccaaag cccgaatggg agcggccgaa gctaagcttc gtccgaatgg gggctcttcg
     6121 cttcgctgtg tcgttgtggg aattaaaccg tgcccttcgc ttcgctgtgt cttagtggga
     6181 aacctttagg atccttgtgg gaaacctctc aagcatcgaa gatgctccac attattacta
     6241 ctcaatcaag taaaaacccc ggcttcgcag aagcccaaca tcttcggcat caacggagca
     6301 agttctcacg caaccccacc ccaatgaaag caccactatc gacaaaaaaa taatattaat
     6361 tttttaatcg ggactgcaat cgttgggctt ctccgaagcc atttcttatt cttatttttt
     6421 accaataagg ttatgcatct ccgatgcc
//
LOCUS       DAOQXT010000174         6433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_12413_length_6433_cov_5.722640, whole genome shotgun sequence.
ACCESSION   DAOQXT010000174 DAOQXT010000000
VERSION     DAOQXT010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12413_length_6433_cov_5.722640"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..642)
                     /locus_tag="PKM27_18590"
     CDS             complement(100..642)
                     /locus_tag="PKM27_18590"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR09335.1"
                     /translation="MDHRRQYLQQLDQLDKTINVWYEKLQPVTALQLRYQAAPGKWSI
                     LHVLAHLVKTEEVVLQYCRKKVQAADLRRVSPMTSLRASLLPLLVGKRLYRYKAPRVV
                     APAPDLEYTGLEDLFTEWEKVRKDWKQFIEQLPEDKLDKEVYRQPNLGRLGWKGTFIF
                     FENHMKRHWKQIEEIVVSSQ"
     gene            678..2270
                     /locus_tag="PKM27_18595"
     CDS             678..2270
                     /locus_tag="PKM27_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR09336.1"
                     /translation="MIRKIPGLFLVLLFVSALKAQVKKAPEIREGEGPYTQLIIRGVT
                     LINGNGAPPIGPVDVVVENNRIRQIKTVGYPGVPIDPADRPKLGPQGRELDASGMYLL
                     PGFIDMHGHIGGRAQGADAEYVFKLWLAHGITTVRDPSAGNGLDWVLDQKTKSNKNLI
                     TAPRILAYTAFGMGSKEPITTAEQARQWVRDNAAKGADGIKFFGANPEIMQAALEENK
                     KLGLRSACHHAQMDVARWNVVQSARAGLTTMEHWYGLPEALFDDRTVQDYPPDYNYAN
                     EQDRFSQAGNLWKQAAQAGSKKWEEVLDELLRLDFTLDPTFNIYEANRDLARARLQEW
                     HQEYTMPSLWRFYTPSRISHGSYWHNWGTEQETQWKENYRIWMQFVNAFKNRGGRVTV
                     GTDSGFIYQLYGFSYPRELELLREAGFHPLEIIRSATLNGAQALGLDKELGTVEVGKL
                     ADFVILDQNPLKNFQVLYGTGAVSLNEKNEVVRIGGVKYTVKDGIIYDAKKLLEDVRI
                     MVAREKSDSGQTIVQPGASKKS"
     gene            2305..3546
                     /locus_tag="PKM27_18600"
     CDS             2305..3546
                     /locus_tag="PKM27_18600"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNR09337.1"
                     /translation="MQFHPLSPATYQAHRNALGKDLDPEGIAILHSNDRMPRTADQYF
                     PFRQNSDLLRLCGIYQGETVLVLFPDHPDPEKRELLFIQRTSEHMQTWDGEALSKVEA
                     IRISGIEQVHWTDQFENLVYPLVARASQVYLNSNEKADYPQNVLNRNERLGLELLRQF
                     PFHTVRRLQPLLKKLSLVKSPEEIEAIRAAARLTGQTWKKISRLIKPGMYEFELAAEI
                     TYQFEKNGAVHAFDPIVASGPSACTLHYKSNHQKIRAEDLILVDFGAELNGYSADMTR
                     CKAVDGTMNARQFAVYDQVVNLFQQARLMLKPGARIQEVNEKIQLWASDALLQLGLAS
                     PVEARDHKIIRKYLPHGISHFLGLDVHDYGDRYLALEPGMVLTCEPGLYIREEGIGIR
                     LENDLLITETGNEDLMADYEI"
     gene            3568..4089
                     /locus_tag="PKM27_18605"
     CDS             3568..4089
                     /locus_tag="PKM27_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR09338.1"
                     /translation="MEKGIKLETERLILIPLTYDQVQKYLRNDLSLEMELGVETQPRE
                     ISPALKEALEETFLPMLADPAKNYLYCTLWTIVLKSENRMVGDLCITGEPKDGEIEIG
                     YGTYTAHQGRGYMVEAVGGLVNWALGQQEVKSVYASTEKTNLASRHILLKNRFKLIRE
                     NGDLYEFRIEAPI"
     gene            complement(4172..5329)
                     /locus_tag="PKM27_18610"
     CDS             complement(4172..5329)
                     /locus_tag="PKM27_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR09339.1"
                     /translation="MNKKSNRRSFFRSSLGALTGMALVKSGTANPIPSTLNQPEFYIN
                     HKLKETIRFSAIGLNHGHIYGQTNALLRAGGELVSFYTKETDLAEAFLKRFPQARLAR
                     SEDEILEDQNIKLVLSASIPLERAPLGIRVMEHGKDFMADKPGIITLEQLAEVKKVQK
                     KTGRIYSIMYSERFENRATVKAGELVQAGAIGQVVQTIGLGPHRMNIASRPPWFFDKK
                     YYGGIICDIGSHQCDQFLYFTGSTKAEIVSSQVGNVHYTRYPEFEDFGDMTVRGNKGT
                     GYIRVDWFTPDGLATWGDGRLTILGTEGYIDIRKNIDIGGKPGGSHLFLVDKKQTQYV
                     DCSKVELPYGAQLIQDVLQRTETAMSQEHCFLATEIALRAQKEAGKLVIEQ"
     gene            complement(5556..5804)
                     /gene="atpC"
                     /locus_tag="PKM27_18615"
     CDS             complement(5556..5804)
                     /gene="atpC"
                     /locus_tag="PKM27_18615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNR09340.1"
                     /translation="MNLIVLTPEQELYHGPIKSVKVPAINGQFEILANHAPIVAALEK
                     GSIRVIDDKGAKSVFSIENGYVEVLQNSVVILVNGVNK"
     gene            complement(5816..>6433)
                     /locus_tag="PKM27_18620"
     CDS             complement(5816..>6433)
                     /locus_tag="PKM27_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNR09341.1"
                     /translation="MPSAVGYQPTLATEMGLMQERITSTKRGSITSVQAVYVPADDLT
                     DPAPATTFAHLDATTVLSRKISALGIYPAVDPLDSTSRILDPLIIGEEHYQCAQRVKN
                     ILQRYNELQDIIAILGMEELSEEDKLIVLRARRVQRFLSQPFFVAEQFTGMKGVLVPI
                     EETIRGFNMIMDGEVDEYPEAAFNLVGTIDEAIEKGKRLIAEAKG"
BASE COUNT         1565 a         1673 c         1640 g         1555 t
ORIGIN      
        1 accgcttccc gaagctaaga ccttctttta tcagatatca tggaatctga ttactgacta
       61 ttgactactg actattgact attgactatt gactattgac tattgactac tgactactat
      121 ttcttcaatc tgcttccaat gccttttcat atgattctca aaaaatataa aggttccttt
      181 ccaacccagc ctgcccaggt tgggctgccg gtaaacctct ttgtccagct tatcttccgg
      241 tagttgctcg atgaattgtt tccaatcctt cctcactttt tcccattcgg tgaataaatc
      301 ctccaggccg gtgtattcca ggtccggggc cggggcaacc actctggggg ccttgtatcg
      361 gtaaagacgt tttccaacca gcagaggcag taaagatgcc ctcagggaag tcatcgggga
      421 aaccctcctc aggtccgctg cctggacttt cttcctgcaa tattgaagga ccacttcttc
      481 cgttttaacc aggtgggcca gcacatgcaa aatggaccac tttcccgggg cggcctggta
      541 cctgagttgg agggccgtaa cgggctgcag cttttcatac cagacattga tagttttatc
      601 caattgatcc agttgctgga ggtattgcct tcgatgatcc attttacaaa atatacctaa
      661 tttggaaaaa aaatgaaatg atccgtaaaa tacccggcct cttcctggtt ttattgtttg
      721 taagtgccct gaaagcccag gtaaaaaagg ccccggaaat ccgcgaaggg gaaggccctt
      781 acacgcaact catcatccgc ggagttacct tgatcaatgg caacggggca cctcccatag
      841 ggcccgtgga tgtcgtggtg gaaaacaacc ggatccgcca gattaaaacg gttggttatc
      901 ccggcgtgcc gatcgatccc gcggaccggc ccaaactggg ccctcagggg agggagttgg
      961 atgccagcgg catgtacttg ttgccgggtt ttattgacat gcacggacat atcggcggca
     1021 gagcccaggg agccgatgca gaatatgttt ttaagctttg gttggcccac gggattacca
     1081 cggtgaggga tcccagtgca ggcaatggac tcgattgggt cctggaccaa aaaacaaaat
     1141 caaataaaaa cctgatcacc gctccccgta tcctcgccta taccgctttt ggcatgggat
     1201 caaaggagcc catcaccacc gcggaacagg cccggcaatg ggtcagggac aatgccgcca
     1261 aaggggcaga cggtatcaaa ttttttggag ccaatccgga aattatgcag gccgctttgg
     1321 aggaaaacaa aaaactgggt ctccgctcag cctgccacca tgctcaaatg gatgtggcca
     1381 gatggaacgt ggttcagtcc gcccgggccg gcctcaccac catggaacac tggtatggcc
     1441 ttcccgaagc gctttttgac gaccgtaccg tccaggatta cccgcccgat tacaattatg
     1501 ccaatgaaca ggaccggttc agccaggctg gcaatctttg gaaacaggcc gcccaggccg
     1561 gaagtaaaaa atgggaagag gtgttggatg aactgctccg gctcgatttt accctggacc
     1621 caactttcaa tatttacgaa gctaaccggg accttgcccg ggccaggctg caggaatggc
     1681 accaggaata caccatgccg agcctttggc gcttttatac cccaagccgc atctcccatg
     1741 gctcttattg gcacaattgg ggtaccgagc aggaaaccca atggaaggag aattaccgca
     1801 tctggatgca atttgtcaac gccttcaaaa accggggtgg ccgggtgacc gtaggcacgg
     1861 actccggttt tatataccag ttgtacggct tttcctatcc ccgggaacta gaactccttc
     1921 gcgaagcggg cttccatcct cttgagatca ttcgcagcgc gaccttaaat ggggcgcagg
     1981 cccttggcct ggacaaggaa ctcggaactg tagaagttgg caaattggcc gattttgtaa
     2041 tcctggatca gaatccgcta aagaacttcc aggttttgta tggtaccggg gcagtctccc
     2101 tgaatgaaaa aaatgaagtg gtccggattg ggggcgtgaa atacaccgtc aaggacggaa
     2161 ttatctatga tgcaaaaaaa ctgctggaag atgtgcggat catggtagcc cgggagaaat
     2221 cggacagcgg ccaaaccatt gtacagcccg gtgcatccaa aaagagttga agcggcctga
     2281 tttttgtctt tattccattg attcatgcag tttcatcccc tttcaccggc tacctaccag
     2341 gcccatagaa acgcgttggg caaggacctg gatccggaag gcattgccat ccttcattcc
     2401 aatgaccgca tgccccggac ggcggaccag tatttccctt tccggcaaaa cagcgatttg
     2461 ctgagactct gtgggatata ccagggcgaa accgtcctgg tcttgtttcc tgaccacccc
     2521 gaccctgaaa aaagggaatt gttgttcatc cagcgtacca gtgaacatat gcagacctgg
     2581 gacggggaag cactgagtaa agtggaagcc atccgcatct cgggaatcga acaagtacac
     2641 tggacggatc agtttgaaaa tttggtttat ccactggtcg ccagggcaag ccaggtctat
     2701 ctcaacagca atgaaaaagc ggactaccct caaaacgtcc taaaccggaa tgaaaggttg
     2761 ggactggaac tgctccgtca attccctttc catacggtcc ggcgtttgca gcccttgctt
     2821 aaaaaactca gcctggtgaa gtcaccggaa gaaatcgaag cgatccgggc cgcggcccgg
     2881 ctgaccggcc agacctggaa aaagatctcc cggctgatca agcccggcat gtatgaattt
     2941 gagctggcgg ctgaaatcac ctatcagttt gaaaaaaacg gcgcggtcca cgcttttgat
     3001 cccattgttg cctcgggccc ttcggcctgt accctgcatt acaaatcgaa ccatcaaaaa
     3061 ataagggcag aggatctcat cctggtggat tttggcgccg aactgaacgg ttacagcgcg
     3121 gatatgaccc gctgcaaagc ggttgacggc actatgaacg cgcgccagtt tgcagtctac
     3181 gaccaggtcg taaacctgtt tcagcaggca agattaatgt tgaaacccgg cgcccggatc
     3241 caggaggtca atgaaaaaat ccagctttgg gcttcggatg cccttttaca attgggtctg
     3301 gcctctccag tggaagcccg ggatcataaa ataatccgga aataccttcc acatgggata
     3361 tcccattttc tcgggctcga tgtacacgat tatggcgacc gctacctggc cctggaaccg
     3421 ggcatggtcc tgacctgcga gcccggcctg tatatccgtg aagaaggcat cggcatccga
     3481 ctggaaaatg acctcctgat cacggaaacc ggcaatgaag acctcatggc agattacgaa
     3541 atttgagatt gactttcttg gtgcgttatg gaaaaaggga ttaaacttga aacggaacgg
     3601 ttgattttaa ttccacttac ctatgatcag gttcagaaat atctccgcaa tgacctctcc
     3661 ctggaaatgg agctcggggt agaaacccaa ccgcgggaga tcagcccggc attgaaagag
     3721 gccctggagg aaaccttcct gcccatgctt gcagatccgg caaaaaatta tctgtactgc
     3781 accctttgga ctatcgtatt gaaatccgaa aaccggatgg tgggtgatct gtgcatcacc
     3841 ggtgaaccga aggatggcga gattgaaatc gggtatggca cctataccgc ccaccaaggc
     3901 cgaggttata tggttgaagc cgtcggtggc ctggtaaact gggccctcgg tcagcaggaa
     3961 gtcaagtccg tatacgcctc cacggaaaaa accaatttag cctcccggca tatcctttta
     4021 aaaaatcgat ttaagctcat tagggaaaac ggggatcttt atgaatttcg aattgaagcc
     4081 ccaatttagg caaaggaatc ctcatatttc caggagcact atccgtattt cattactcgc
     4141 agctcatgtc tcgcagctcg tggctcgcag ctcattgctc tattaccaat ttccccgcct
     4201 ctttctgagc ccttaatgca atttccgtgg caaggaaaca atgctcctgg ctcattgcgg
     4261 tttcggtgcg ctggaggacg tcctggatga gttgcgctcc gtaaggaagt tcgactttgg
     4321 agcaatccac atactgggtt tgttttttat ccaccaggaa aagatggctg ccgccgggct
     4381 ttcccccgat atcgatgttt ttacggatat caatataacc ctcggtccca aggatggtaa
     4441 gccttccatc gccccaggta gccaggccgt ccggcgtgaa ccagtccacc cgaatatatc
     4501 ccgtgccttt atttcccctt accgtcatat ccccgaaatc ctcaaattca gggtaccggg
     4561 tataatgtac gttcccaacc tgggacgata caatctccgc tttcgtggat ccggtaaaat
     4621 ataaaaactg atcgcattga tgggacccga tatcgcagat gattccgcca taatattttt
     4681 tatcaaagaa ccagggggga cggctggcta tgttcatccg gtgaggtccc agtccaatgg
     4741 tctgcaccac ttgtccgata gcccctgcct ggaccagttc cccggcttta acggttgccc
     4801 ggttttcaaa ccgttcactg tacatgatgg aatagatccg gccggttttt ttctggactt
     4861 ttttcacttc agccagttgt tccagggtga tgatccccgg cttgtcggcc ataaaatcct
     4921 tgccatgttc catcacccgg atacccaggg gtgcccgttc cagcgggatg gaggcgctta
     4981 aaaccagttt gatgttttga tcttccagga tctcgtcttc cgaccgggcc aatcgggctt
     5041 gcgggaaccg tttgagaaaa gcttcggcga ggtccgtttc ctttgtatag aaggaaacca
     5101 gctcaccgcc cgccctcaac agggcattgg tctgcccata gatatgcccg tggttgagcc
     5161 cgatggcgga aaagcggatg gtttctttca atttgtgatt gatataaaat tctggctggt
     5221 tcagcgtcga tggaatcggg tttgcagtac ccgatttaac cagggccatg ccggtaaggg
     5281 ctccaagaga ggaccggaaa aaacttcgcc ggtttgattt tttattcatg ttcaatgagg
     5341 ttgtggcatt aaagatatac cggatttcga aaaaagggag ggtcaaagga tggggcgtgg
     5401 gtgaaagccc cttactcagt ggcaaaagta ttatgccttt agacatgtgg gaacccgtcg
     5461 agggtcaaat acccagcaat attgccccct tagacttaaa acacctaaag cgattcatcc
     5521 gggcggcctc gcttacgacc gggcaaaaga ccgttttatt tattcacgcc gttgaccagg
     5581 atgactacgc tgttctggag cacttcaaca tatccgtttt caatgctgaa aacggatttc
     5641 gcccctttgt catcaatcac ccggatcgat cctttttcca gggcggctac gatgggtgcg
     5701 tgattggcga ggatctcgaa ttgcccgttg atagcgggca ccttaacgga tttaatgggt
     5761 ccgtggtaca attcctgttc cggtgtgagg acaatcagat tcatatggtt gcggcttaac
     5821 cttttgcttc ggcgatcagt cttttgcctt tttcaatggc ctcatcaatg gttccgacca
     5881 ggttgaaagc cgcttcggga tattcatcca cctctccgtc catgatcata ttaaacccgc
     5941 ggatggtttc ttcaatgggg accagcactc ctttcattcc ggtaaactgc tcggcaacaa
     6001 agaaaggctg ggacaggaac cgctgtacgc gccgtgcgcg caatacgatc agtttgtctt
     6061 cctccgacaa ttcttccata cccaggatgg cgatgatgtc ctgcaactca ttgtaacgct
     6121 gtaaaatatt tttcacccgt tgcgcgcatt gataatgttc ctccccgatg atcagggggt
     6181 cgaggatcct ggacgtggaa tccagcgggt ccaccgcggg gtaaataccc agcgcggaga
     6241 ttttgcggct taatacggtg gtggcatcga ggtgggcaaa agtggtggcc ggcgcagggt
     6301 ccgtaaggtc atcagcgggt acatagaccg cctggacgga ggtaatggag ccccgcttgg
     6361 tcgaggtgat ccgctcctgc atcaagccca tctcggtggc cagcgtcggt tggtaaccta
     6421 ccgcggaagg cat
//
LOCUS       DAOQXT010000175         6343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_12630_length_6343_cov_7.177322, whole genome shotgun sequence.
ACCESSION   DAOQXT010000175 DAOQXT010000000
VERSION     DAOQXT010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12630_length_6343_cov_7.177322"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..2256)
                     /locus_tag="PKM27_18625"
     CDS             complement(91..2256)
                     /locus_tag="PKM27_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNR09342.1"
                     /translation="MNRLFLMLLTLGVFPSFAQRQNSNYRYPMKRIDQAMIIDGHEGD
                     AAWKSLDIATDFYMVLPMDTSKAKVRTDVRMCYDAKNIYLLVEAYHAIDKNYQVESLK
                     RDFAFGKNDNFLLFMDPYDDGTNGFSFGSNAAGGQWDGTMYEGGKVDLSWENRWFGVT
                     KNYEDKWVFEAAIPFKSIRYKKGITEWGINFSRLDIRTTEKSSWAPVPRIFPTASLAY
                     TGILVWDQPPPDPGLNVSFIPYALTGYSRNFEKNTKAHFRKEIGGDAKISLGSALNLD
                     LTVNPDFSQVEVDQQVTNLDRFELFFPERRQFFLENGDLFANLGNSTLRPFFSRRIGL
                     GIPIDFGARLSGKLDQNWRIGILDMQTRKNEFQPGYNFGMFALQRKVFSRSNITVFAV
                     NKQAVGYDKLSAELQKTNSQYNRNLGFEYNLASANNRWTGKFMYAKSLDSKKSPDSWS
                     HVGNLAYATRTLSMTWQQEYVSKNYTAEVGYVPRREYWRISPMVSYLFYPAKSKVLTH
                     GPSLGTFAYFTPGLKTTDRTVFLSYNFNFRSQAKASVWVGDDWVRLLQPFDPTNTRKD
                     TLARGTEHKWRSTGADFYSQPQKLFTYSFSYRVGGYYQNGNRTNFATELGYRFQPYVK
                     LALTANYNNISLPSPWYRTVFWLVGPRADVTFTNNLYFTTFVQYNEQVNNININTRLQ
                     WRFKPASDLFIVYTDNYLPAPFNAKDRAVVIKMNYWWNM"
     gene            2363..4807
                     /locus_tag="PKM27_18630"
     CDS             2363..4807
                     /locus_tag="PKM27_18630"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNR09343.1"
                     /translation="MRYFNDRSPSTHHPPSTIHLLPLLAFLVMCKSVHLPAPVRLPNG
                     WSVSPAGKSHSLGDLPLNMAISPDGQFMVATNSGQGEHSLQLIDLQEEKVVDNFIVPV
                     TWVGAAFSGHRDFYISGGTTNKVFHYEIVNKKIKYLDEIALGDPWPKAIWPGGLSIDP
                     VRKLLYTVTKEDKSLYIVDLAAKKVNHRVELGAEGYTCILSKDFSILYISLWGKDEVL
                     SFDTRSNQVIQSIPVGNHPNDMCISQDGHFLYVANANDNSVSILNLADQKVIETLNAA
                     LYPGSITGSGTNGVALDPDQKTLYIANADNNCLAAFDVSQPGRSKSLGFIPTGWYPTS
                     VKVVKNKIWVANGKGFQSAANPQGPSPVNPNENVTYQQGDTTKPDEIQYIGGLFRGNL
                     SIIDIPDTKTLAQYTQRVIQNIPYKKETELVANGRTGNPIPKKVGDPSPIKYVFYIIK
                     ENRTYDQVMGDVKEGNGDSTLCLFPEPVTPNQHALAREFVLLDNFYVDAEVSADGHNW
                     STGAYATDYVEKTWPTSYGGRGGTYDFEGTRKLTWPQKGYIWDHCKRAGVSYRTYGEY
                     GDRGKATIPALEGHVSPGFTGWNMSVRDTTRFSQWKHDFDSLLARNAVPEFNSIRMGN
                     DHTEGLRKGRPSPFAHVADNDLAVGLFVEHLSKSRIWNESVVFILEDDAQNGPDHVDA
                     HRSIAFVAGGFVKRGFVDHTMYSTSSMLRTIELILGIPPMSQYDASATPMWRCFSSKA
                     DNRPYQHRIPDADLDEKNTAMNKWQKISETFDLSKEDAVHDMLFNEVLWYAVKGERHA
                     YPGPRRAAFLTVEEDE"
     gene            4978..6306
                     /locus_tag="PKM27_18635"
     CDS             4978..6306
                     /locus_tag="PKM27_18635"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNR09344.1"
                     /translation="MTQLNQRITLYGLTMIAVGSSIGSGIFVTPGTIAQEIPNAWLIL
                     LVWLLGGLVGLCGALTFSELGSLFTRSGGVYVYLRHAYGPLAGFLYGWVMLVVINTGS
                     LAGLCVAFAEYLKFFLPGLSQGMKMAVAAGTMVILTGINILGVHYSQWLSNVLTTLKV
                     GAILIIVIIGLFAGNEEVIRQNFNLAAPVPGHLFTALFSGMIGVFFSVGGWHHATYVA
                     GEVINPGKNVARAMILGTGVITLAYVTINLAYLRLLPVESIAGTQTAASDAVSTVFPW
                     AGRLVAIGIALSIFGTISIYTMSAPRIYFAMAEDGLFFKPMARVHPRWRTPVTAMLVQ
                     AVWALCILFFFQAYFDRIITFVTFMDALFFALAAASVFVFRNKLKGEPRPYRVWGYPV
                     IPLVFIILYTVFAMNIFFQKPQQAIPGLVLMGLGIGLYYWFSRIKREIRE"
BASE COUNT         1556 a         1613 c         1593 g         1581 t
ORIGIN      
        1 ccaatcctat taatcctacc aatcctacca atcctattaa tcctaccaat cctactaatc
       61 ctaccaatcc tattaatcct attaatccta tcacatgttc caccaataat tcatcttaat
      121 caccactgcc cggtccttgg cattgaacgg agcaggaagg taattgtccg tataaacgat
      181 aaacaggtcc gaggccggct taaacctcca ttgaaggcgg gtattgatat tgatgttatt
      241 gacctgttcg ttgtattgca cgaaggtggt aaaataaagg ttgttggtga aggtgacatc
      301 cgccctgggg cccaccagcc agaataccgt gcggtaccag ggggagggca ggctgatgtt
      361 gttgtaattc gccgtcaggg ccagctttac atagggttgg aagcgatagc ccagctcggt
      421 ggcaaaattg gtgcggttgc cgttctggta atatccgcct acccggtaag aaaacgaata
      481 ggtgaagagc ttttgaggct gggaataaaa atcagctccc gtcgatctcc atttgtgttc
      541 ggtgcccctg gcgagggtat ctttccgggt attggtagga tcaaagggtt gcagcaagcg
      601 gacccagtca tcaccaaccc agacggaagc cttggcctgg ctcctgaaat taaaattata
      661 actcaggaat accgtacggt cggtcgtttt caatcccggg gtaaagtagg caaaggtgcc
      721 aagggaaggc ccgtgggtca acactttact tttggctgga taaaacaggt aagagaccat
      781 ggggcttatc cgccagtatt cccggcgggg cacatatccc acctcggcgg tataattttt
      841 actgacatat tcctgttgcc aggtcatcga aagggtccgg gtggcatagg ccaggttgcc
      901 aacatgggac caggagtcgg gggatttttt tgaatccagg gatttggcat acatgaattt
      961 tccggtccac cggttgttag cagaagccag gttgtattca aagcccaggt tgcggttgta
     1021 ttgcgaattg gttttttgca gttccgcgct tagcttgtca taacctaccg cctgcttatt
     1081 cactgcaaag acggtgatgt tggagcggct gaagaccttg cgctgaaggg caaacattcc
     1141 aaaattgtat ccgggctgaa attcgttttt ccgggtctgc atatccagga tgccgatacg
     1201 ccaattctgg tccaacttcc cgctcaagcg tgcaccgaag tcaataggaa tgcccaggcc
     1261 gatacgcctg gagaaaaagg gccggagggt gctgttgccc aggttggcga agagatcccc
     1321 gttttcaaga aaaaactgcc ttcgttccgg gaaaaacaat tcaaagcggt cgaggttggt
     1381 cacctgctgg tccacttcca cctgtgaaaa atcgggattt accgtgaggt ccaggtttag
     1441 ggcgctcccc agggatatct tggcatcccc cccgatctct tttcggaaat gggccttggt
     1501 attcttctca aaattcctgg aataccctgt cagcgcatag ggaataaaag atacgttcag
     1561 gcccggatca gggggcggct ggtcccatac gagaatgccg gtataagcga gggaagccgt
     1621 cggaaagatc ctgggcaccg gcgcccagct cgatttttcg gtggtccgga tatccagccg
     1681 ggaaaaattg atgccccatt cggtaatgcc ttttttgtac cggatgcttt tgaaaggaat
     1741 ggccgcttca aatacccatt tatcctcgta atttttggtc accccaaacc agcggttttc
     1801 ccagctcagg tccaccttgc ccccttcata catggtgccg tcccattgcc cgcccgccgc
     1861 attggaaccg aatgaaaagc cattggtgcc gtcatcgtag ggatccatga agaggagaaa
     1921 gttgtcgttt ttgccaaagg cgaaatcccg cttgagcgac tccacttggt aatttttatc
     1981 aatggcatgg taagcttcca ccaggaggta gatgtttttg gcatcgtaac acatccgcac
     2041 gtccgtacgc actttggctt tgctggtgtc catgggcagt accatataga agtcggtggc
     2101 aatgtccagg gatttccagg cggcatcccc ttcgtgacca tcaatgatca tggcctggtc
     2161 aatccttttc atggggtacc ggtaattgga attttgcctt tgcgcaaagg aagggaagac
     2221 ccccaaagtc agcagcatca aaaacaatct attcatcatc ttgggattgc aaaaataggg
     2281 tttctgacta tgcctctctc tttgattttc atattgttcc aaaagagcct gaatatttga
     2341 tcacattatg tatttttaac ctatgcgcta ttttaacgac cgttctccct ctacccacca
     2401 cccaccatcc accatccacc tcctccccct ccttgccttt ctcgtgatgt gcaaatccgt
     2461 ccatctgccg gctccggtca gattgcccaa cggatggtca gtcagccctg ccggtaaaag
     2521 tcattccctg ggcgatctcc ctctaaatat ggccatctcc cccgatggac aatttatggt
     2581 ggcgaccaac agcgggcaag gtgaacacag cctgcaactc atcgacctcc aggaagaaaa
     2641 agtcgtcgac aattttatcg taccggtcac ctgggtcggg gctgcatttt ccggtcatcg
     2701 cgatttttac atcagcgggg ggaccaccaa caaagtattt cattatgaga tcgtcaataa
     2761 aaaaataaag tacctggatg aaattgccct gggagatccc tggccaaaag cgatctggcc
     2821 cggaggttta agtattgacc ccgtcagaaa attgttgtac acggttacca aagaagataa
     2881 aagcctttac atcgtcgacc tggccgccaa aaaagtcaat caccgggtgg aactgggagc
     2941 cgaagggtat acctgtatac tgtcaaaaga tttcagcatc ctgtacatca gtctttgggg
     3001 caaagacgaa gtattgtcgt ttgatacccg gtccaatcag gtcatccaat cgatcccggt
     3061 ggggaaccac ccaaacgaca tgtgcatcag ccaggatggt cattttcttt atgtggccaa
     3121 cgccaatgac aacagcgttt ccatccttaa cctggcggac caaaaagtca ttgaaacgct
     3181 gaacgccgct ttataccccg ggtcaatcac gggaagcggt accaacgggg tagcccttga
     3241 cccggatcaa aagacccttt acatagccaa tgcggacaat aattgcctgg ccgcgtttga
     3301 cgtcagccag cccggaagaa gtaaaagcct gggttttatt cccacgggtt ggtatcccac
     3361 ctcggtcaaa gtggtgaaaa ataaaatctg ggtggccaat ggcaaaggat ttcaatcggc
     3421 tgccaatccc caaggcccca gtccggtcaa ccccaatgaa aatgtcacct atcagcaagg
     3481 ggacacgacc aagcccgatg aaatccagta catcggaggt ttattcaggg gaaacctcag
     3541 catcatagat atcccggata ctaagaccct ggcacaatat acccaaaggg tgatacagaa
     3601 tatcccctat aaaaaagaaa ctgaactggt cgccaatgga cgcaccggga atcccatacc
     3661 taaaaaagtg ggggatccct ccccgatcaa atatgtattt tatatcatca aggaaaaccg
     3721 aacttatgac caggtcatgg gcgatgtaaa agaaggaaac ggggattcga ccctttgttt
     3781 atttccagaa ccggtaaccc caaaccagca tgctctggcc agggagtttg tattgctgga
     3841 taatttttat gtcgatgcgg aggtcagcgc cgacgggcac aattggagca ccggggccta
     3901 tgccactgat tatgtagaaa agacctggcc cacgagttat gggggacggg gaggcactta
     3961 tgattttgaa ggcactcgaa aattaacctg gccgcagaaa ggttatatct gggatcattg
     4021 caaacgcgcg ggggtcagct accgcactta tggcgaatat ggagaccggg gaaaagctac
     4081 cattcctgct ttggaagggc atgtaagccc gggatttacg ggttggaaca tgtcggtaag
     4141 ggataccacg cggttcagcc aatggaaaca tgattttgat tcattattgg caaggaacgc
     4201 ggtcccggaa ttcaacagca tccgcatggg caatgatcat accgaagggc ttcgcaaagg
     4261 caggccaagc ccttttgccc atgtagcgga caacgacctt gccgttgggc tgtttgttga
     4321 acatctgagt aagagcagga tctggaatga atcggtcgtg tttatcctgg aagacgatgc
     4381 ccagaacggc ccggaccatg tagatgcaca ccggagcatc gcttttgtag ccgggggctt
     4441 tgtaaaaaga ggatttgtgg accataccat gtattccaca tcctcgatgc tcagaactat
     4501 tgaactcatc ctcggaatac cacccatgag ccagtatgat gcttccgcca cccccatgtg
     4561 gcgctgtttt agttccaaag cagataaccg tccttatcaa caccggatac cggatgcgga
     4621 tctcgatgaa aaaaatacgg ccatgaacaa atggcaaaag atttccgaga cctttgattt
     4681 gtccaaagaa gacgcagtgc atgatatgtt gttcaatgaa gtcctttggt atgcggtgaa
     4741 aggggagcgt catgcctatc ccggaccacg aagggcagcg ttcttgacgg tggaggaaga
     4801 tgaatgaaaa ttaaaaaatt aagaattaag aattaaggcc cccctcttaa ccttaacctt
     4861 aatcttaacc ttaaccttaa cctcaacctc aaccttaacc ttaacctcaa cctcaacctt
     4921 aaccttaacc tctttacatc tttgcgcctt tgcgagaaat aaacttaccg agaaaccttg
     4981 acccagctaa accaacgcat caccctctac ggcctcacca tgatcgccgt gggttccagc
     5041 atcggttccg gcatttttgt cacgcccggc acgatagccc aggaaatacc caatgcctgg
     5101 ctgatcctcc tggtctggct cctgggggga ctggtggggc tttgcggggc cctgaccttt
     5161 tcggagctgg gaagtttatt tacgcgttcc ggaggcgttt acgtttacct caggcatgcc
     5221 tacgggcctt tggcaggatt tctgtatggt tgggtcatgc tggtggtgat caatacggga
     5281 tccctggcgg gtttatgtgt ggcctttgcg gaatacctga agttttttct tcccggtctt
     5341 tcacagggaa tgaagatggc cgtggcggcg ggtaccatgg tcatcctgac cggaatcaat
     5401 atcctggggg tgcattacag tcaatggttg tccaatgtcc tcaccacgct caaagtgggc
     5461 gccatcctca tcatcgttat aatcggttta ttcgccggga atgaggaggt gatccgccaa
     5521 aactttaacc ttgcggcccc tgtacccggg cacttgttta ccgccctctt cagcgggatg
     5581 attggggtat ttttttcagt gggaggctgg caccacgcca cctatgtcgc cggcgaggtg
     5641 atcaacccgg gcaaaaacgt agcccgcgcc atgatcctcg gcaccggcgt catcaccctg
     5701 gcttatgtga ccatcaacct ggcctacctg aggttgctgc cggtggagtc catagccggc
     5761 acccagaccg ccgcgagcga tgccgtgtcc accgtatttc cctgggcggg ccggctggtg
     5821 gccatcggga tcgccttatc cattttcggc accatcagca tatacaccat gagcgccccc
     5881 aggatctatt ttgcgatggc ggaggatggt ttgtttttca aacccatggc cagggtgcac
     5941 ccgcggtggc ggacccctgt caccgccatg ctggtgcagg ccgtatgggc tttatgcatc
     6001 ctgttttttt tccaggccta ttttgaccgg atcatcactt ttgtcacttt catggacgcc
     6061 cttttcttcg cgctggccgc ggcctcggta tttgttttca ggaataaatt aaaaggggag
     6121 cccaggcctt accgggtctg gggctatccg gtcatccccc tggtttttat cattttatat
     6181 acggtttttg ctatgaatat cttttttcag aaacctcaac aagccattcc ggggctggtt
     6241 ttgatgggtt tggggattgg gctgtattat tggttcagta gaataaaaag ggaaattcgg
     6301 gagtaaggtc attttttaga ccgaccgaat tttattttct tgt
//
LOCUS       DAOQXT010000176         6296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_12744_length_6296_cov_6.090530, whole genome shotgun sequence.
ACCESSION   DAOQXT010000176 DAOQXT010000000
VERSION     DAOQXT010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12744_length_6296_cov_6.090530"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..2565)
                     /locus_tag="PKM27_18640"
     CDS             complement(130..2565)
                     /locus_tag="PKM27_18640"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNR09345.1"
                     /translation="MGEFFKSIGLAVLFILFHHEVPGQDPLRDIKAYLASNAPALQAE
                     PVDLEDLVISDSHEDAGTGLKYVYVQQRYHGIPIENALLNFTFREGKLVFANTDRLVH
                     GISSKIKSTRFSISPASAAATALARSVGDSYLRSLPRLVREESGNKYVFQKESNIQED
                     IPVALIYKTDAEGHLGLYWKAGVHESSGDYWNNYIDARTGKIESRTNLVLSCFSHEDH
                     PAGAPVAPTLPYLPFNPLATQYKVWPLPISAPNAGSPSLIADPMDLAASPLGWHDDGV
                     QKFQITRGNNVHAYADPDSNMITANDEPNGGAGLSFDFPFNPNGTIASNKNAAVVNLF
                     YMNNVMHDFAWHYGFTEEAGNFQAKNLSGKGRGNDWVIALSQFGANSNRHRNNADFLA
                     PGDGSNGRMRMFVWNQSGSKLFKVIAPASLAREFETGSAEFGPNISTVPLSGKLVLVS
                     DGSNNPTLGCKSLVNAAAVKGNIALINRGDCFFQEKVCFAQAAGAIGAIIGNYENTPI
                     TMGDVNPAPCSVNIPSLSVSSLDFAFLKNNIQTITVSFQAPATVNVDKDASFDNGIIA
                     HEYGHGISIRLTGGPSNSGCLGNDEQMGEGWSDFFTLATTVKAGDNRNTQYGIGTYPL
                     QQDVSGRGIRKYPYSLDFGINNLTYEDILTTETPHSLGEVWTMMLWELYWAMSETYGW
                     DANLYKGTGGNNKAIRLVFEGLKLQPCNPGFVDGRDAILQADKLLYNGENECLIWKAF
                     AKRGLGFSAKQGSSSDRSDGVEAFDLPPSCIPTVKISKKMTPLIKAGDDIAVNFDHMQ
                     GGQLCQQGAGNRT"
     gene            2689..3420
                     /gene="rlmB"
                     /locus_tag="PKM27_18645"
     CDS             2689..3420
                     /gene="rlmB"
                     /locus_tag="PKM27_18645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNR09346.1"
                     /translation="MIYGKHPVLEALKEEVPIEKIMLLQGTHGELEKSLRETCRERSI
                     PLQQVPREVLFKLAKGNNHQGIAAIISPVEYQDLGGLLPFILSQPTVPLFILLDRVTD
                     VRNLGAIARSAELLGVDAIVLSAKNRARIDEEAIKASAGALLKLAVCRENSLSAAIDL
                     LHKEGIKIAAADSHTGMPIREVDFTQPCALLLGSEDEGVHPSLLEKSDYRFRIPQLGT
                     LDSFNVSVAAGIALYEIYNQRYKTN"
     gene            3500..4453
                     /locus_tag="PKM27_18650"
     CDS             3500..4453
                     /locus_tag="PKM27_18650"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNR09347.1"
                     /translation="MKKIFRSTLYLSMALGLVQFTACSGARSTVSAQSGQAAAYVPLE
                     KSLLWEISGNGLTKPSYLFGTIHLIDKSEFFFPATLEEKLKACERATFEIDMDEMSSM
                     ESMFKIIGKAFMKDGLTLRDLLTEAEYKVVEDHFSEKGLPLQFLDRMKPMLLATFASL
                     DLGQGGLSDTKSYEMEIFARVNEQNKPSDGLETVDDQLSIFDSIPYQTQAKMLMESIK
                     SSDTENEEYKKLIELYKKQDIETLVKISVGEGDGGVGEHADLFLYQRNRNWIPHMSRM
                     MKEKPTFFAVGAGHLGGPQGVINLLKKAGFKVSAVKMGLGA"
     gene            4502..5635
                     /locus_tag="PKM27_18655"
     CDS             4502..5635
                     /locus_tag="PKM27_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNR09348.1"
                     /translation="MPPYHPDTQAIHAGYRSDDFYGAVAMPIILTTTFERGPDGKFVE
                     GRDIYARSSNPNRRMLEDKLTLLEGGQGAAAFSSGQAATMSIFHALGAGSHILIPDDI
                     YYGTRVLLEKLYGPWGASFTAVDMTDIDRVKAAIQDNTRLIWIESPSNPSLKVTDIEA
                     VASLAREKNILTGCDNTWASSFFCRPLSVGVDVVMHSSTKYFGGHSDLTGGCVIWGSN
                     AALSQKIRDFQTLGGAVPSAFDSWLLFRSLSTYPLRMKKHGENALQLAEFLESHPAIE
                     KVHYPGLPSDRFHTLASKQMHGGYGGMVSILVKGGAEEALHLASRLQLVCHATSLGGV
                     ESLVDHRQTAEGVHSLSPPNLLRISVGLEHISDLIADFKQALG"
     gene            complement(5813..6229)
                     /locus_tag="PKM27_18660"
     CDS             complement(5813..6229)
                     /locus_tag="PKM27_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HNR09349.1"
                     /translation="MAIKRTSSARWEGNGKDGKGTVSTPSGVLNDTFFSYKTRFEDAI
                     GTNPEELVAAAHAGCFSMKLAFGFQAENLVPESIETTSTVVLDEGWIKEAHLVTRVKV
                     SGIDHEKFLSLVQFAKENCPISKSLKAEITIEAEMV"
BASE COUNT         1491 a         1637 c         1679 g         1489 t
ORIGIN      
        1 cccatgtcca aacacattgc ccgcaaacgc ttcggtcaaa attttctgca agacccccgc
       61 accatcgccg acattgtcga agccgtccgc cctgcttacg acgacctcgt gattgaaatt
      121 ggccccggct taggtgcgat tacccgcccc ctgctggcac aactggccac cctgcatgtg
      181 gtcgaaattg accgcgatat catcgcccgc cttgatcagg ggggtcatct ttttactgat
      241 tttgacggtc gggatgcagg atggcgggag atcgaaggct tccactccgt cgctccggtc
      301 agaggaggac ccttgtttgg cgctaaatcc aagtcccctc ttggcaaagg ctttccagat
      361 caggcattcg ttctcgccgt tatagagcag tttgtccgcc tggaggatgg cgtcccttcc
      421 atccacgaat ccgggattgc aaggttggag tttaaggcct tcaaagacca gccgtatggc
      481 tttattgttt ccaccggttc ccttgtaaag gttggcatcc cagccatagg tttcagacat
      541 ggcccagtac aattcccaga gcatcatcgt ccatacttca cccagcgaat ggggagtttc
      601 cgtagtcagg atatcctcat aagtcaggtt gttgatacca aaatccagcg aatagggata
      661 tttgcggatt cccctgcccg atacatcctg ctggagcgga taggtaccga tgccgtactg
      721 ggtattgcgg ttatccccgg ctttcaccgt ggtcgccagg gtgaaaaaat cactccatcc
      781 ttctcccatc tgttcgtcat tacccaggca gccggagttg gaggggccgc ccgtgagccg
      841 gatggagatc ccatgtccgt attcatgcgc aataattcca ttgtcaaagg aggcatcctt
      901 gtccacattg acggtcgccg gcgcctgaaa actgaccgta atggtttgaa tattgttctt
      961 taaaaaggcg aagtccagcg aactgaccga caaggacggg atgttgacac tacagggtgc
     1021 gggattgacg tcgcccatgg ttatgggtgt attttcataa ttgccgatga tggccccgat
     1081 tgccccggcg gcctgggcaa aacatacctt ttcctggaag aaacaatccc cccggttgat
     1141 gagcgcgata tttcctttca cggcggccgc attgaccagg cttttgcagc ccagggtggg
     1201 attgttggat ccgtcggata cgaggaccag tttgcctgag agcggtacgg tggagatatt
     1261 tggtccaaat tcagcagacc cggtctcaaa ttcccgggcc agagatgccg gggcgatcac
     1321 tttaaacagt ttggacccgc tttgattcca gacaaacatg cgcatcctgc cgttggatcc
     1381 gtccccagga gccaggaaat ccgcgttgtt ccggtgcctg ttactgttgg ccccaaattg
     1441 ggagagggcg atcacccaat catttcccct gcccttgccg ctgaggtttt tagcctggaa
     1501 attcccggct tcttccgtaa aaccgtaatg ccaggcaaaa tcatgcatca cgttgttcat
     1561 gtaaaacagg ttgaccaccg cggcgttttt attggatgca atggtgccgt tggggttaaa
     1621 aggaaagtca aacgaaagac cggccccccc gttgggttcg tcgttggcgg tgatcatgtt
     1681 tgaatcgggg tcggcatagg cgtgaacatt gtttccgcgg gtgatttgaa acttctgcac
     1741 cccatcgtca tgccatccca atggggaggc ggccaggtcc ataggatccg cgatcaggga
     1801 cggactgccg gcattgggcg cgctgatggg caggggccat accttgtact gggtcgcaag
     1861 ggggttgaat ggaaggtaag gaagtgttgg tgcgactgga gcgccagccg gatgatcctc
     1921 gtgcgaaaaa caggacagca cgagattggt tctggattca atcttcccgg tgcgggcatc
     1981 gatatagttg ttccaataat cgccggagct ttcatggacc ccggctttcc aatataaacc
     2041 cagatggcct tccgcatcgg ttttataaat gagggccacg gggatatctt cctggatatt
     2101 ggactctttt tggaaaacat atttattccc ggattcttcc cggaccagtc ggggcaggga
     2161 gcggagataa gaatcaccca ccgatcgagc cagggctgtg gctgccgctg aagccgggga
     2221 gatcgaaaac cgggtgcttt tgatcttgga cgaaatgccg tgaaccagtc ggtccgtatt
     2281 ggcaaatacg agtttccctt ccctgaaggt gaaattgagc aaggcatttt ctatgggtat
     2341 cccatggtag cgctgctgaa cgtaaacata ttttaagccg gtccccgcat cttcatggga
     2401 atcgctgatc accaggtctt ccaggtcaac gggttcagcc tggagcgcag gggcattcga
     2461 agccaggtaa gccttgatgt cacggagcgg atcctggccc ggaacctcgt ggtgaaataa
     2521 aataaagagc actgcaagac ctatgctttt gaaaaattca cccatgaaaa aattgtagtt
     2581 ttatacgcaa atttaaagcc tccggggatg gaactgcttc atcggtgcaa aagtcgtttt
     2641 ttcgtatctc taaattaagc taatattttg ttaaataact caggtcaggt gatctacgga
     2701 aagcatccgg tgctggaagc tttaaaagaa gaggtgccta ttgaaaagat catgttgttg
     2761 cagggaaccc atggcgagct tgaaaaatcc ctgcgtgaaa cctgccgtga acggtccata
     2821 cccctgcagc aagtgccccg ggaagtattg ttcaaactgg caaaaggcaa caaccaccag
     2881 gggattgcag ccatcatttc cccggtggaa taccaggacc tgggaggact gctccccttt
     2941 attctttccc agcctaccgt ccccctgttt atcctgctgg accgcgtcac cgatgttcgc
     3001 aacctggggg ccatagcccg cagtgccgag ttgctgggcg tagatgcgat cgtgctctct
     3061 gccaaaaacc gcgcaaggat agatgaagaa gcgatcaaag cttctgccgg ggcccttctg
     3121 aaactggcgg tctgccgtga aaacagcctc agcgcggcca tagacctcct ccataaagaa
     3181 ggaataaaaa tcgcggcagc ggattcccat accggcatgc cgatccggga ggtggatttt
     3241 acccaaccct gcgcactgtt attgggctcc gaagacgaag gcgtgcatcc ctccctgttg
     3301 gaaaagtcgg attaccggtt caggatcccc caactgggca ccctggattc attcaatgta
     3361 tccgttgccg cgggcattgc actttatgaa atatataacc aacgctataa aaccaattga
     3421 cgtttaattt gcattttaaa acaggtgcag ggcatggagg acgtgttttt ccagtggatc
     3481 tgtgccggga aaaccaaaca tgaaaaaaat attcagatcc accctttacc tgtccatggc
     3541 ccttggcctg gtccagttta ccgcctgttc cggcgccagg tctacggtta gcgcgcagag
     3601 cggccaggcc gcggcctatg tcccgcttga aaagtccctg ttgtgggaaa tctccggaaa
     3661 tggactgaca aaaccttcct acctgtttgg taccatccac ctcattgaca aatcggaatt
     3721 tttcttcccc gccaccctgg aagaaaaatt gaaggcctgt gaacgcgcca ccttcgagat
     3781 cgatatggac gaaatgtcca gcatggagtc gatgttcaag atcatcggca aagcttttat
     3841 gaaagacggc ctgaccctga gggacctctt gaccgaagcg gaatacaaag tggttgaaga
     3901 ccatttcagc gaaaaaggcc ttccgctcca gttcctggac cgcatgaaac ccatgctgct
     3961 ggccaccttt gcttccctgg acctgggaca gggcggcctg tcggacacca aatcctatga
     4021 aatggaaatc tttgcccggg tcaacgagca gaacaaacct tccgacggac tggagaccgt
     4081 ggatgaccag ctttccattt ttgattccat cccttatcag acccaggcga aaatgctgat
     4141 ggaaagcatt aaatcttcgg acaccgaaaa cgaagaatac aaaaaactga tcgagctgta
     4201 taaaaagcag gatatcgaaa ccctcgtgaa aatatcggtg ggtgaaggag acggaggggt
     4261 aggcgaacat gccgatttat ttttgtatca gcgaaaccgc aactggatcc cgcacatgtc
     4321 caggatgatg aaggaaaagc ctactttttt tgctgtagga gccgggcact taggagggcc
     4381 tcagggcgtc attaacttat taaagaaggc aggcttcaag gtcagcgcgg taaaaatggg
     4441 cctgggggcc tgagcgtatc ggaacagata acttgaggga aatccgttta aaaaagtaaa
     4501 tatgccacct tatcatccgg atacgcaagc catccatgcc ggataccgga gcgatgattt
     4561 ttatggggcg gtcgccatgc ccattattct gaccaccacc ttcgaacggg gaccggatgg
     4621 caaatttgtg gaaggccgtg acatctatgc ccgcagttcc aaccccaacc ggagaatgtt
     4681 ggaagacaag cttaccttgc ttgagggggg acagggcgct gccgcctttt catccgggca
     4741 ggcggctacg atgagcatat ttcatgccct gggtgcggga tcccatatcc tcatcccgga
     4801 cgatatctat tacggtaccc gcgtattgct ggaaaaatta tacggtccat ggggggcctc
     4861 gttcaccgcg gtggatatga ccgatatcga ccgggtgaag gcagccattc aggacaatac
     4921 ccggttgatc tggattgaaa gcccatccaa tccttccctt aaggtgaccg atattgaagc
     4981 ggttgcttcc ctggcgcgtg aaaaaaatat cctcaccggc tgcgacaaca cctgggcaag
     5041 ttcttttttc tgccggcctc tttccgtggg ggtggatgtg gtgatgcatt ccagcacaaa
     5101 atattttggg ggccacagcg acctcacggg aggttgtgtg atctggggat caaacgctgc
     5161 cttaagtcag aagatcaggg attttcagac cctcgggggc gccgtccctt cggctttcga
     5221 cagttggctc ctgtttcgaa gcctgtccac ttacccgctg cgcatgaaaa aacacgggga
     5281 gaatgccctc cagttggctg aattcctgga atcccatccc gccatcgaaa aagtccatta
     5341 ccccgggctg ccttccgacc gttttcatac cctggcttca aaacaaatgc acgggggata
     5401 tgggggaatg gtttccatcc tggtcaaagg aggagccgaa gaagccctgc acctggcctc
     5461 caggctccaa ctggtttgtc atgccacttc gctcggagga gtggaaagcc tcgtcgatca
     5521 ccggcagacc gcggagggcg tacacagcct cagtccacca aacctgctgc gtatctcagt
     5581 ggggctggaa cacatcagtg atctgattgc ggactttaag caggcgctgg ggtaggggtt
     5641 ggaagcaaga agctgggagt tagaagctgg aagctggtgc gtttgcgcaa accacctcac
     5701 cgctacgcct cttaagcaca gcgaccgact ttaattttgc aggaatacaa ggagcagggc
     5761 cccctagctc ctagcttcta gcttctaact tctaactcat agctgaaagc ccctaaacca
     5821 tttccgcctc tatcgtaatc tccgccttta aggatttgga gatcgggcaa ttctctttgg
     5881 caaattgcac caggctgagg aatttttcgt ggtcgatgcc ggagaccttc acgcgggtga
     5941 ccaggtgggc ttccttgatc cagccttcat cgaggacgac ggtggaagtg gtttcaatgg
     6001 attccgggac caggttttcg gcctggaatc cgaaagccag tttcatggaa aaacaacccg
     6061 catgggccgc ggccaccagt tcttccggat tggtgcctat ggcatcctca aaacgggttt
     6121 tgtaagaaaa aaaagtatcg ttcaatactc cgctgggcgt gctgaccgtg ccttttccgt
     6181 ctttgccatt gccttcccaa cgtgcgctcg aagtcctttt gatagccata tttttgattt
     6241 tttagttgat ggttttataa aattcatgca aaggtaagga aagtggtgga tggtgg
//
LOCUS       DAOQXT010000177         6163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_13054_length_6163_cov_5.281434, whole genome shotgun sequence.
ACCESSION   DAOQXT010000177 DAOQXT010000000
VERSION     DAOQXT010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13054_length_6163_cov_5.281434"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1556
                     /locus_tag="PKM27_18665"
     CDS             147..1556
                     /locus_tag="PKM27_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNR09350.1"
                     /translation="MKQRFFEPSLYHTRRNKLAQALQDGLIVFPSNEAAGMNYRDNTY
                     PYRQDSSFLYFCGIDRPGLFLFIDSQSGESILAGDESTMDDVVWTGPVESLHELSEHA
                     GLDRVITLAEAKNWISNLIERNIRIHYLPVYRGDQAFRLAALLDTGAEAISGNASIPL
                     IEAVVSLREIKEEPEIKELSDAINISRQMHITMMKSACAGMTEAEVMARVRAECLKHD
                     LDIAYPIILTVNGQTLHNHAYHNILEHGQLLLGDFGSESSMHYAGDITRTIPVSRHFT
                     PLQKDIYSLVLQTLSSSTAAIREGIRYLDVHLQAARIIAGGLKDLGFLNGNPDDLVAE
                     GVHALFFPHGLGHALGLDVHDMENLGEKYTGYESGLERSTQFGLRSLRMAKTLKAGMV
                     LTVEPGIYFIPELVALWKKNHSYPGLVNYDRIERNLPFGGIRIEDNVLVTPDRAQVLG
                     DPIPKEIADIEQYRELAYS"
     gene            1580..2371
                     /locus_tag="PKM27_18670"
     CDS             1580..2371
                     /locus_tag="PKM27_18670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR09351.1"
                     /translation="MKTFLSEYRHDYNTYTFGYTIYCVCEERSELDDIYDQGFLPYTG
                     DLGLKYDLFYKSRGIRVRLADFKDSSENRRVNRKMESLGITFEMIPVESYERIEQFVE
                     FARKYSEERIGEDKMPLQRIRYILGRRYLTHILPFTSAGVIIGYVLAVVTDRQFHYWF
                     SFYDTRFLDQNKPLGKWLMWKCIHIARELGKEHIYLGNGYLEKSLYKTRDFKSVEFYD
                     GNSWSGDLKRLQQLCLSDAVLPELDAFKLNDRRNEWIAAEMARKK"
     gene            complement(2375..3379)
                     /locus_tag="PKM27_18675"
     CDS             complement(2375..3379)
                     /locus_tag="PKM27_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNR09352.1"
                     /translation="MNKLFLGLLLFTAVQLAHGQATIGKIHIVKPAGEMLIDPQAPIE
                     VLADGFDWSEGPLWWKEENAVLFSDVPANTVYIWRPNGEGALPYIKPSGYTGVGKYSR
                     EPGSNGLTRDLNGNLLLCEHGDRRISYLPLNKPGGKRTLTDNHNGKRYNSPNDVIVDS
                     KGRVYFTDPPYGLPNQDKDASRETEAFGVYLVDQGKTSLVVNDLVRPNGLALSPDEKF
                     LYVGQSDGAKPHIMKYPVMANGLLGKGTLFFDCSDLAKQGLRGAPDGMKVDDQGNVWS
                     TGPGGVLVISPKGDLLARIETGQATANCAWGDDGSTLYMTADRYLCRVKTKVKGVPFR
                     "
     gene            complement(3392..4741)
                     /locus_tag="PKM27_18680"
     CDS             complement(3392..4741)
                     /locus_tag="PKM27_18680"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNR09353.1"
                     /translation="MRIHVIAMGGAVMHNLSIALHLAGHQVSGSDDEIYNPAKDRLQA
                     HGLLPAPGWDPDKLDAGVELVILGMHAKKDNPELAKAQELKLRILSYPEFIALHATNK
                     RRVVVAGSHGKTTTTAMIMHVLRHLGTAFDYLIGAPLEGFDHMIRLSDAPLMVIEGDE
                     YLSSPIDPVPKIIHYRPHFTVITGIAWDHINVFPTKNDYLEVFRNYLSQLPEQAIVWY
                     YQGDEDLCRVVEACPRTDLAFLPYQTPYHEIREGLSILVRDGKEYPMQVFGQHNMQNA
                     EAALQVCLALGCRETDVLSALTSFRGAAKRLQALYASGQTRIYLDFAHAPSKVRATVQ
                     AIRTQYPDSRLVAILELHTFSSLNKNFLSQYRNTLDPADEAVIFYNKHTLEIKRMPDL
                     DPEEIKSQFDRPDLFIFTENAQLENYLNTLTKTQAVILLMSSGTFNGVDIKSWAQKE"
     gene            complement(4834..>6163)
                     /locus_tag="PKM27_18685"
     CDS             complement(4834..>6163)
                     /locus_tag="PKM27_18685"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNR09354.1"
                     /translation="DGVITGYGLIHGRLVYVFAQDFTVFGGSLSETHARKICVLMDMA
                     LQNKAPMIGLNDSGGARIQEGVDSLAGYAEVFYRNTLASGVIPQISAILGPCAGGAVY
                     SPAITDFILMTDHTSYMFVTGPNVVKTVTNESVTSESLGGALSHAGKSGVSHLTAAND
                     MECLEMIKKLLFYLPSHHEAPSPQYPFDLGDEYRPALDGVLPESNHLPYDIKTVIREV
                     VDRDSFFEIHELFAQNVVVGFATLAGKSIGIVANQPQVLAGVLDVNASRKAARFVRFC
                     DSFNIPLIVLVDVPGFLPGTDQEWNGIIVHGAKLLYAFSEATVPRLTVIVRKAYGGAY
                     DVMNSKHIGADFSFAWPGAEIAVMGARGASEIIFKKEIESAADPVAKVLEKEKEYAET
                     FLHPYRAAARGYVDEVILPSETRRKLIKALASLSKKAVARPSKKHGNIPL"
BASE COUNT         1463 a         1652 c         1555 g         1493 t
ORIGIN      
        1 gtacctccct tcctcccttc ctccatttca tcctcccgcg gctgcgggac tcccttcctc
       61 cctttgaatc ctaggaatag ttttattttt gtttcccatg gaccccatta acaaatttga
      121 cttttaatcc cctcaacctg ttcatcatga agcaacggtt tttcgaacct tccctttatc
      181 atacccgcag aaacaagctg gcccaggccc tgcaggatgg tttgatcgtg ttcccctcca
      241 atgaagcagc cggaatgaat taccgggaca atacctatcc gtaccggcag gacagttctt
      301 tcttatattt ctgcggcata gacaggcccg gattattcct gttcattgac agtcaaagcg
      361 gggaatccat tttggccggt gatgaaagca ccatggacga tgtggtctgg accgggcccg
      421 tggaatccct gcatgaactt tcggaacatg ccggccttga ccgggtcatt accctggcgg
      481 aggcaaaaaa ctggatctcc aacctgatcg aacgaaacat caggatccac taccttccgg
      541 tataccgggg agaccaggcc tttcgactgg ctgcattact ggataccggg gccgaggcca
      601 tctccgggaa tgcatccata cccctcatcg aagcagtggt gagcctgcgt gaaatcaagg
      661 aagaaccgga aatcaaagaa ctgagcgatg ccataaacat cagccgccag atgcatatca
      721 ccatgatgaa gtccgcgtgc gcagggatga ccgaggcgga agtcatggcc cgggtaaggg
      781 cagaatgtct caaacatgac ctggatatcg catatcccat catcctcacc gtaaacggac
      841 agaccctcca caatcacgcc tatcacaata tcctcgaaca tgggcaactg ctccttggtg
      901 attttggatc tgaatcgtcc atgcattatg cgggggacat cacccggacc ataccggtga
      961 gcaggcattt taccccactc caaaaggata tttactccct tgtcctgcag acgctgtcct
     1021 cttccaccgc agcgatcagg gaaggcatcc gatacctcga tgtacatctt caggcggcca
     1081 ggatcattgc cggtggattg aaagatcttg gttttctgaa tggaaatccc gatgacctgg
     1141 tcgctgaagg ggtgcatgcc ttattttttc cccacggatt gggccatgcc ctgggtctgg
     1201 acgtgcacga tatggaaaac cttggcgaaa agtacaccgg ttatgaatcc ggtctggaaa
     1261 ggagcacgca gtttgggctg cggtccctgc gtatggccaa aacgctgaaa gcagggatgg
     1321 tcctgacggt tgaaccgggg atctatttta tacctgaact ggtcgctttg tggaaaaaga
     1381 accattctta tcccggcttg gtcaattacg accggatcga acgaaacctc ccgtttggcg
     1441 gcatccggat cgaagataat gtcctggtca cacctgaccg ggcccaggtc ctgggagacc
     1501 ctatccccaa agaaatcgcc gacatcgaac aataccgtga actggcctat tcctgaccgg
     1561 ttggcttggg gctgaatgca tgaaaacttt cttatcagaa taccgccacg attacaatac
     1621 ctataccttc gggtatacaa tctattgtgt ctgtgaagaa aggtcggaac tggatgatat
     1681 ttatgaccag ggctttctcc cctacaccgg agaccttgga ctcaaatacg atttatttta
     1741 caaatccagg gggatccggg tccgtcttgc ggatttcaaa gattccagcg aaaaccgcag
     1801 ggtcaaccgg aaaatggaat ccctgggcat caccttcgaa atgatcccgg tcgagtccta
     1861 tgaacggatc gaacaatttg ttgaattcgc ccgaaaatac agcgaagaaa ggatcggaga
     1921 ggacaagatg ccattgcagc ggatccgcta tatcctgggg cgcaggtacc tcacgcatat
     1981 tcttcccttt acctcagccg gggtgatcat cggatatgtc ctcgcggtgg tcaccgaccg
     2041 ccagtttcat tattggtttt ccttctatga tacccgattc ctggatcaaa acaaacccct
     2101 tggaaaatgg ctgatgtgga aatgcatcca tatcgcccgt gaactgggca aggaacacat
     2161 ctacctgggt aacggctacc ttgaaaaatc cttgtataag acccgggatt ttaaatccgt
     2221 ggaattttat gacggcaact cctggtccgg cgacctgaaa cgacttcaac aactctgcct
     2281 ttccgatgcg gtcttgccgg aacttgatgc gtttaaatta aacgacagac gcaatgaatg
     2341 gatcgcggcc gagatggccc ggaaaaagta aaacctacct gaaagggacc cctttcacct
     2401 tggtttttac ccggcacaag taacggtcgg ccgtcatgta taaggtgctg ccgtcatccc
     2461 cccaggcaca attggcggtg gcttgtccgg tttctatccg ggccagcagg tcgcccttgg
     2521 gggaaatcac caataccccg cccgggcccg tactccacac gttgccctgg tcatccactt
     2581 tcatgccatc cggcgcaccc ctcaatcctt gtttcgccag gtcgctgcag tcaaagaaaa
     2641 gcgttccttt tcctaaaagc ccgttggcca tcacggggta tttcatgatg tggggtttgg
     2701 ccccgtccga ttgtccgacg tataaaaatt tttcgtccgg agacaaagcc agcccattgg
     2761 gcctcaccag gtcattgact acgaggctcg tttttccctg gtcaaccagg taaaccccaa
     2821 atgcctccgt ttccctgctg gcgtctttgt cctgattggg cagaccataa ggggggtcgg
     2881 taaaatatac cctccctttt gaatcgacga tgacgtcatt tggcgaattg taccgtttgc
     2941 cgttgtggtt atccgtgagg gtgcgtttcc cacccggctt attgagaggc aggtaagaga
     3001 tcctccggtc cccatgttca cagagcagga ggtttccatt cagatcacgg gtaagtccgt
     3061 tgctgccggg ttccctgctg tattttccaa ccccggtata gccgctgggt ttaatataag
     3121 gcagggcgcc ttccccgttc ggcctccaga tgtaaaccgt attggccggc acatcgctga
     3181 ataaaacggc gttttcttcc ttccaccata aggggccttc tgaccagtca aagccgtcag
     3241 caagcacctc aatgggtgcc tggggatcga taagcatttc accggcgggt tttacaatat
     3301 ggatcttgcc gatggtcgcc tggccatggg ccagctgcac ggccgtaaaa agcaataatc
     3361 ccagaaataa tttgttcata gatcttgtta tttattcctt ttgcgcccag cttttgatat
     3421 ccactccatt gaaagtgccg ctgctcatca gtaaaatgac ggcctgcgtt ttcgttaagg
     3481 tattcaggta gttttccagc tgcgcgtttt cagtaaaaat aaacaaatcc ggacggtcaa
     3541 attgggattt tatctcctcc ggatcgaggt cgggcatacg cttgatttca agggtgtgct
     3601 tgttgtaaaa aatcaccgcc tcatccgccg gatccagggt attccggtat tgggataaaa
     3661 agtttttgtt caggctgctg aaggtgtgga gttccaggat tgcgaccaac cggctgtccg
     3721 ggtattgggt gcgaatagcc tggaccgtcg cccttacttt tgacggggca tgtgcaaaat
     3781 caaggtaaat cctggtttgc ccggaggcgt aaagagcctg gagccgtttt gccgcacccc
     3841 ggaaagaggt cagagcggaa agcacatccg tttcacgaca acccaaggcc aggcaaacct
     3901 gcagcgccgc ctcggcattc tgcatattgt gctggccaaa gacctgcatc gggtattcct
     3961 tcccatcccg gacaaggatg gacaagcctt ccctgatttc atggtaaggg gtttgataag
     4021 gaaggaaggc caggtccgtc cgtgggcagg cttccacgac gcggcataag tcttcatctc
     4081 cctggtaata ccaaacgatg gcttgttccg gaagctgaga caaataattt ctgaatacct
     4141 ccagatagtc attctttgtt gggaaaacat tgatatggtc ccaggcaatg ccggtaatga
     4201 cggtgaagtg aggcctgtaa tggatgattt tagggacagg atctatgggt gagctgagat
     4261 attcatcccc ttcgatcacc atcagggggg catccgacaa acggatcata tgatcaaatc
     4321 cttccagggg cgctccgatc aggtagtcaa atgcagtccc cagatgcctg agcacatgca
     4381 tgatcatggc tgtggtcgtg gtcttgccgt ggcttccggc gactaccacc cttcttttgt
     4441 tcgtagcatg cagggctata aattccgggt aagacagaat ccgcaacttc agctcctggg
     4501 cttttgccag ctcaggattg tctttttttg catgcatgcc gaggatcacc agttcaaccc
     4561 cagcatccaa tttgtccgga tcccatcctg gtgcgggcag gaggccatgg gcctggagcc
     4621 ggtctttggc gggattataa atttcatcgt ctgatccact gacctggtgt cccgccaggt
     4681 gaagggcaat gctcaggtta tgcatgaccg ctccacccat ggctattaca tgaattcgca
     4741 tgcggcaaaa ttggaaaaaa taaaggtcat attaaacttt ttttgcatct gaatggggcg
     4801 gatccaggat cagggggtca atccaataag ggattaaagg gggatgttcc cgtgtttttt
     4861 cgaaggcctg gccactgcct ttttcgatag gctggccaat gctttaatga gttttcgccg
     4921 ggtctccgaa ggaaggatca cctcatccac ataaccccgg gccgccgccc ggtatggatg
     4981 caaaaaggtc tcagcatact ctttttcttt ttccagtact ttggcgacgg ggtctgcggc
     5041 agattcgatc tctttcttaa atatgatttc gctggcgccc ctggccccca tgactgcgat
     5101 ttctgccccc ggccaggcaa agctgaaatc agccccaatg tgttttgaat tcatcacgtc
     5161 ataggctccc ccgtaggcct ttcttacgat caccgtaagc ctgggcaccg tggcttcgct
     5221 gaaagcatac aacaacttcg ccccatgtac gatgatgccg ttccattcct gatccgttcc
     5281 gggtaaaaat ccgggaacat ccaccagtac gatgagaggt atattgaatg aatcgcaaaa
     5341 gcggacgaac cgggcggcct ttcttgaagc gtttacatcg aggaccccgg ccaggacctg
     5401 gggctgattg gcaacgatac caatcgattt tccggccagg gtggcaaatc ccacgaccac
     5461 attctgagca aaaagttcgt ggatctcaaa aaacgaatcc cggtcaacga cttcgcgaat
     5521 gaccgtcttg atgtcatatg gcaaatggtt ggattcgggc aagacaccgt ccagtgcggg
     5581 ccggtactca tcccccaggt cgaatggata ttgcgggctg ggcgcttcat gatgggaggg
     5641 aaggtaaaac aaaagttttt tgatcatttc aaggcattcc atgtcgttgg ctgccgtgag
     5701 atgcgagacc ccggatttcc ccgcatggct cagcgcaccg cccagggatt cgctggtgac
     5761 ggattcattc gtcaccgttt tcaccacatt cggcccggta acaaacatat aggaagtatg
     5821 gtccgtcatc agaataaaat cagtgatggc cggtgaatac acggccccgc ccgcacaggg
     5881 accaaggatg gccgaaatct gtggaatgac cccggaggca agggtattcc tgtaaaagac
     5941 ctccgcgtaa ccggccagcg agtctacccc ctcctggatc ctggcacccc cggaatcatt
     6001 caaaccgatc atgggagcct tgttttgaag ggccatgtcc atgaggacac agattttccg
     6061 ggcatgggtt tcagacaagg aacctccaaa aacggtaaaa tcctgggcaa aaacataaac
     6121 caagcgcccg tgaatgagtc cgtagcccgt gatgacccca tcc
//
LOCUS       DAOQXT010000178         6110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_13196_length_6110_cov_5.022791, whole genome shotgun sequence.
ACCESSION   DAOQXT010000178 DAOQXT010000000
VERSION     DAOQXT010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13196_length_6110_cov_5.022791"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1409)
                     /locus_tag="PKM27_18690"
     CDS             complement(204..1409)
                     /locus_tag="PKM27_18690"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNR09355.1"
                     /translation="METLGNPTTTKVQKDLEKTAREIGPEISKYVEEEETNRRLSPSV
                     VRTLRNAGFFKMFQPEAVGGLETDPLTAARVIEEVARHNTAAAWSLMVTNTSPIMLVK
                     LPDDGINAIYGNHPDVFVAGSVHPPMSAIPVKGGYSITGRNPLFSNVHEAHWLVVLAF
                     VMENGQMKMQHGHPEIIAAVMKASDCEIIDTWHTISMKGTDSNDVSAKDVFVPDYLSL
                     PLNPELAPNPRFQGPLYRFPASGANIACLFPPVSLALARNAINELKSLAEKKTPLGSM
                     VSIKQKGAMQRKLGIAEAMVQSSRAYLYEAIEECWEKVKAGNEVSREEKARLLLAATH
                     TNQTCTKAVDLMYSAAGSTAIYTRNKIAHYFTDAQVLKQHGFVNDSRYETAAQIYFGL
                     PADLPLVEF"
     gene            complement(1839..2213)
                     /locus_tag="PKM27_18695"
     CDS             complement(1839..2213)
                     /locus_tag="PKM27_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNR09356.1"
                     /translation="MQRLPDLYIAHAGLKGRGVFTAHDIPAGSLIEICPLILIPGTQA
                     PLIDRTEIYNYYFVWNEGRLALALGYGSLYNHDPAPNARVVCDYESDEIQIEAIRDIG
                     PGEEITIHYRDEEGFKGKLWFE"
     gene            complement(2215..3093)
                     /locus_tag="PKM27_18700"
     CDS             complement(2215..3093)
                     /locus_tag="PKM27_18700"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNR09357.1"
                     /translation="MPLKIKRDDASFLLIVLGCIWGSSFILIKRILPCFNPVQFALLR
                     IILTGLCFTPWFLSSFKKINRKQFSWLLLLGLMGYFFAYALMGLSQTHISSSLAGILA
                     SLNPLATFIIGIAFFRQSFRWKNFWGIALGLLGSLVLILFDRGHGDISVGFYSLFMIG
                     SVIFSSITINITQNHLAGLSPYVIASVSFVGMALPALVLFASTADWTSLAAHPQILTC
                     LMAAATLALVGTVIGSLLYYYIISKAGALYTSTVTYIIPLVAILWGLIDGELVHWNSL
                     LGLGLIIGGIKLIRST"
     gene            complement(3115..>4131)
                     /locus_tag="PKM27_18705"
     CDS             complement(3115..>4131)
                     /locus_tag="PKM27_18705"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNR09358.1"
                     /translation="SLDQSLPDLTPQDLSIAGELRRGESMHATLRIQNIGNWTMAAIR
                     DTVYLSLDSLFSNDDLPVALASLPELAKNQAAQVDINLDIPDQARSGTQYLLLTVNRN
                     SLLAEKNKSNNSIYKKINIPNRKADLNLRGLQLTKNTLAAGDTLYITDFMENIGETAA
                     GNYRVLHSIKKSPQDQVPLVTFQAILYSGLEPAGSTRLASFAQIPEQLKPDTYYLISC
                     LDADGVIDEIRKDNNCGQLLFTVQKKEQTTPMKNDRIFTTFKSFPNPVIHELQIQGTF
                     IQSLSSIRLTIRNYAGGEVYRRNLPPGRDLREKIDLSQLPAGVYYFDLASEKGSWKQP
                     FIKL"
     assembly_gap    4132..4231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4232..>6110)
                     /locus_tag="PKM27_18710"
     CDS             complement(<4232..>6110)
                     /locus_tag="PKM27_18710"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNR09359.1"
                     /translation="RQQTSFTYNLPGNSKSGTYYFITCADQTAGVPEFSEGNNCRSTS
                     IELKLKDAPSIYPDLVPALLSSMPQTVTTGQKLVLDGKVSNQGSSYANAHILEMVLSS
                     DDRLSGNDLSLFKETLGDLNPAQSVNFKASLTIPSSLELDHYYLLICSDPSNNIQESS
                     ESNNCLSFALTIFSPRPDLLIKNTSINPNPVPSGVPFTVQFTTSNDGTVNSPVSRTAL
                     YLSKSNNIKDVNDVLLGQISLNQGLAAGAGRSDRFTVSNLIAPGNYFILICADNQEVI
                     KETNEANNCTAISVSITTPMPDLVTDLVTVPDKFYIEDSHDVKIVVRNVGAANASATR
                     GQLTLFSAELPPLLLKTFNIPALGPNESVTLLEKVAVRDLAWLGQKKLSTCVDHERQV
                     TESQETNNCREINLSLIEPLPDLQLGSSALFDEVLVTGTHLTLPFEVTNPGQRPAPVS
                     FTGIYYSRKEMVIDSTSLALLPDSLPSVSPGDTLRVLKEVLIPEPLKPGTWYLTLCLD
                     SRKEVKELSEQNNCQSFRLELRNPMADLAVDTFNASADSVFTSLPFPVKAIISNLGEI
                     PAENFVVEVYLASGSAAGLAASFKLEELNPGTPWDTTLFLVLPDSLFGQKTDISIHLD
                     PG"
BASE COUNT         1432 a         1378 c         1717 g         1483 t
ORIGIN      
        1 gtcagtagtc aatagtcagt agtcaatagt caatagtcag tagtcaatag tcagtagaca
       61 atagacaata gacaatagac agggtatttg ttcttgaatt tttaattcgg gacaccattt
      121 cttttccact ctccgagcta tgacaaaccg tacatttagt tcctttcctt ataccgtgat
      181 ttaattaaca ctcgcgcaac gctctaaaac tcaacgagag gcaaatcagc gggtaatcca
      241 aagtaaatct gggcggcagt ttcgtagcgg ctgtcgttga cgaatccatg ctgcttcagg
      301 acctgcgcat cggtaaaata atgggcaatt ttattccggg tataaatggc cgtgcttcct
      361 gcggcggaat acattaaatc cacagctttg gtgcaagttt ggttggtatg cgtagcggct
      421 aaaagcaacc tcgctttttc ttcacggctg acttcattgc ccgctttgac cttttcccag
      481 cattcttcga tagcctcgta aaggtaggcg cggctggatt gtaccatggc ttctgcgata
      541 ccaagtttac gctgcatcgc tcctttttgt ttgatggaca ccatggagcc gaggggtgtt
      601 ttcttttccg ccagggactt taattcattg atggcatttc tggccaaggc aagcgatacg
      661 ggagggaaaa ggcaggcaat attggcgccg ctggctggaa accggtagag cggcccctgg
      721 aaacggggat tgggtgcaag ttccggattg agggggagcg ataaataatc cggcacaaat
      781 acatccttgg cagaaacgtc attgctgtcg gtccccttca tactgatggt gtgccaggtg
      841 tcaatgattt cgcaatcgct tgccttcatg acggcggcga ttatttccgg atggccatgc
      901 tgcattttca tctggccatt ttccatgaca aaagccaaca cgaccagcca atgagcttca
      961 tgcacattgc tgaaaagagg gtttctgccc gtaatggaat aaccgccttt gaccgggatt
     1021 gcgctcatag gaggatggac tgagccggca acaaaaacat cagggtggtt tccgtaaatg
     1081 gcgttgatgc catcgtctgg taatttgaca agcatgatgg ggctggtatt ggtgaccatc
     1141 agcgaccatg cggctgccgt gttgtgcctg gccacttctt caatcaccct ggcagcggta
     1201 agggggtcgg tttccagacc tccgacggct tcgggctgga acatcttgaa gaagccggca
     1261 tttctcaagg tccttacaac agacggggaa agccttcggt tcgtctcctc ttcttcaacg
     1321 tacttgctga tttcaggtcc tatttcacgc gcggttttct caaggtcttt ttgcaccttg
     1381 gttgtggtgg gatttcctaa agtttccatg tgaatataaa tttgattaaa caatacgtat
     1441 acaatcggca aaaagggtac ccccctaata gcagcgccta ttgatgaacc gcacaatcct
     1501 tgcaaggaaa ggaatgttcc atatgcctgg ggtttgactg tctaaagtta ataattacgg
     1561 gattcgaaaa tcaagggaat aattttcctt gaatttttaa ccttttgtgt tcggtaccgg
     1621 ttgacacttt aagacgcgaa taaaaactct acccattctg ttgaattcac cgggcctttg
     1681 cgatttttct tcgcgaccat tgcgttctcg gcggttaaaa atgtttagat taacccgcaa
     1741 agaccaccag tagcgcgacg gttccgcaaa ggaattaggt gaaactagcc aactgaccac
     1801 tgattgctga ctgctgatta ctgacttctg accactgtct actcaaacca caatttccct
     1861 ttaaacccct cttcatcccg gtaatgaatc gtgatctctt ctccgggacc gatgtcgcgg
     1921 atcgcttcga tctggatctc atcgctttca taatcacaca ctacgcgggc attgggcgcc
     1981 gggtcgtgat tgtacaagga accgtaaccc agagcgaggg cgagccgtcc ttcgttccat
     2041 acgaaataat aattgtaaat ctctgtgcgg tcgataagcg gagcctgagt acccggaatc
     2101 agaatgaggg gacagatttc gatcaggctg ccggcgggaa tgtcgtgggc ggtgaagact
     2161 ccccgtcctt tgagcccggc gtgggcgatg tagaggtcgg gcaggcgttg catatcaggt
     2221 ggaacggatc agtttgatgc ccccgatgat caggcccagc ccaagcaggc tgttccagtg
     2281 gaccagttcc ccgtcgatca ggccccagag aatggcaact aaaggaatga tgtaggtgac
     2341 ggtgctggta taaagggctc ctgccttgct gatgatgtaa taatacaaaa gcgaaccgat
     2401 tacggtgcct accagcgcca aggtggcggc ggccatcagg caggtcagga tctgcggatg
     2461 ggcggccagg ctggtccagt ccgccgtcga ggcaaagagg accagggccg gcagggccat
     2521 gccgacaaag gagacggagg caatcacgta aggcgataat ccggccaggt gattttgggt
     2581 gatgttgatg gtgatggatg aaaagatcac ggaaccgatc ataaacaggc tgtaaaagcc
     2641 gacggaaata tcgccatggc cccggtcaaa caggatcagc accagggagc ccaacaggcc
     2701 cagggcaatg ccccaaaagt ttttccatcg gaaggattgc cggaaaaaag cgataccaat
     2761 gataaaagtg gccagggggt tgagggaagc caggataccg gccaggctgc tgctgatatg
     2821 ggtctggctc aggcccatca gggcataggc aaaaaaataa cccatcaggc ccagcagtaa
     2881 aagccaggag aattgtttcc ggtttatttt tttaaaagaa gataaaaacc agggggtaaa
     2941 acaaaggccg gtgaggatga tccgcagcag ggcaaactgg acgggattga agcagggaag
     3001 gatccttttg atcaggatga atgaactgcc ccaaatgcaa cccaatacga tgagcaggaa
     3061 agaggcgtca tcgcgtttga tttttaaagg cattgccaag ggtaccgggc ggaattacag
     3121 tttgatgaaa ggttgtttcc agcttccttt ttccgaagcc aggtcaaaat aatagacgcc
     3181 ggcaggcagt tggctgagat caatcttttc cctgaggtcc ctgcccggcg gaaggttgcg
     3241 ccggtacact tccccgcccg cataattgcg gatggtcagc cggatcgagg acagggactg
     3301 gatgaaggtc ccctgtatct gaagctcatg gatgaccggg tttggaaagg atttgaaggt
     3361 cgtgaagatc cggtcgttct tcatcggggt ggtctgttcc tttttttgga cggtaaaaag
     3421 aagttgcccg caattattgt ccttccggat ctcgtcgatg acgccatcag cgtccaggca
     3481 tgaaatcagg taataggtat ccggtttcag ttgttccggg atctgggcaa aggaggccag
     3541 ccgggtggaa cccgccggtt ccaaacctga ataaaggata gcctggaagg ttaccagggg
     3601 aacctggtcc tggggtgatt ttttaatgga atggagcacc cggtaatttc cggctgccgt
     3661 ctccccgatg ttttccataa aatcggtaat gtatagagta tcgcctgccg ctagggtgtt
     3721 tttggtcaac tggaggcccc tgagattcag gtccgctttg cggttgggga tgtttatttt
     3781 tttataaatg gaattattgg atttattttt ttctgccagc aggctattgc ggttgaccgt
     3841 cagcaaaagg tattgagtgc ccgacctggc ttggtccgga atgtcaaggt tgatatcgac
     3901 ctgggcagcc tggttcttgg ccagctcggg aagtgaagcc agggctacag gaagatcatc
     3961 gtttgaaaac aggctgtcca aagagagata aacggtatcc cggatggccg ccatggtcca
     4021 gttgccgata ttttgtatcc gaagggtggc gtgcatcgat tccccgcgtc tcagctcgcc
     4081 ggcgatcgac aagtcctggg gcgtgagatc cggcaggctc tggtcaaggc tnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncctggatcg aggtggatac tgatatcggt
     4261 tttctgcccg aaaaggctgt cggggaggac caggaacagg gtagtgtccc aaggggttcc
     4321 tggattcaat tcctctaact tgaaggaagc ggccagcccg gccgcggaac ccgaggccag
     4381 atagacttca acgacaaagt tttcagcagg aatttcaccc aggtttgaaa ttatggcctt
     4441 gaccgggaag gggaggcttg taaatacgga atccgcactg gcattgaagg tgtcgacggc
     4501 caggtcggcc atggggtttc tgagttccag ccggaaactc tggcaattgt tttgttcgga
     4561 tagttctttg acttccttcc ttgaatccag gcaaagggtc aggtaccagg tgcccggctt
     4621 cagtggttcg ggaatcagca cttctttgag gaccctgaga gtatcgccag gggaaacgga
     4681 aggcaggctg tcggggagta gggccaggga ggtggagtct atgaccattt ccttcctgga
     4741 ataatagatt ccggtgaaag agaccggggc cggacgctgg cccggattgg tcacttcaaa
     4801 gggcagcgtg aggtgggtac cggttaccag gacctcatcg aacagggctg aagatcccag
     4861 ctgcaggtcg gggaggggtt ctatcaggga aagattgatt tccctgcaat tatttgtttc
     4921 ctgggactcc gtgacctgcc tttcatgatc gacgcaggtg cttagttttt tttgtccgag
     4981 ccaggccagg tcccggaccg cgaccttttc cagcagggtt acggattcgt tggggcccag
     5041 ggcagggata ttgaaggttt tcagaagcag gggtggaagt tcggcgctga aaagagtcaa
     5101 ttgacctctg gtggcggatg cattcgctgc ccccacattc ctgaccacga tcttcacgtc
     5161 atggctgtct tcaatgtaaa acttgtccgg aacggttacc aggtcggtta ccaggtcggg
     5221 catgggtgtg gtgatggata cggagatagc ggtacaattg tttgcctcgt tggtctcctt
     5281 gatgacttcc tggttgtctg cacaaataag gataaaataa ttgcctggag cgatcaggtt
     5341 gctcacggta aaccggtcgc ttcggccggc accggcggcc aggccctggt tgaggctgat
     5401 ctggccaagc aggacgtcgt tgacgtcctt gatattgttg ctttttgaca aatagagggc
     5461 agtcctggag acggggctgt ttaccgtccc gtcattgctg gtggtaaatt ggaccgtgaa
     5521 cgggaccccg gaaggcacgg gattgggatt gatgctggta tttttaatca ataagtccgg
     5581 tctgggcgaa aaaatggtca gggcgaagga caaacaattg ttggattcgg aggattcctg
     5641 aatgttattg gaaggatcgc tgcagatcaa taggtaataa tgatccagtt cgagactgga
     5701 agggatggtc agggaggctt tgaaattaac ggattgggcc gggttcaggt cccccaacgt
     5761 ttccttgaac aggcttaggt cattgcccga tagccggtca tcggaagaca ggaccatttc
     5821 caaaatatgg gcattggcat aagaactacc ctggttggaa actttgccgt ccagcaccag
     5881 cttttgaccc gtggtcaccg tttggggcat gctggataga agggccggga ccaggtcggg
     5941 gtatatggac ggggcatcct ttaacttgag ttcaatactg gtggaccggc aattgttgcc
     6001 ttctgaaaac tcaggaacgc ctgccgtctg atcggcacaa gtgataaaat aataggttcc
     6061 tgacttgctg tttccgggaa ggttataggt aaaactggtt tgttgccggc
//
LOCUS       DAOQXT010000179         6024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_13433_length_6024_cov_6.077232, whole genome shotgun sequence.
ACCESSION   DAOQXT010000179 DAOQXT010000000
VERSION     DAOQXT010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13433_length_6024_cov_6.077232"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKM27_18715"
     CDS             <1..810
                     /locus_tag="PKM27_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09360.1"
                     /translation="RLPGNKLSIYKQKINDGWIPLTQGQGRNVHIDVYDYSGNKSQVK
                     FYLQGNGSAPEQKIRASHPIAYDQPFTEDREDLKLLFPANSLYTDIDLQLDRSVRKES
                     WSAVYRVHDPSTPLHTSYVISLKPEGIPSRLKNKCVIGLFNGQSVTNCGGTWNGAWLE
                     TKINAFGQFGVVVDTLPPVITPVSFKTDMKSSSLMKFRITDNFEVAGQADRLTYNATI
                     DDQWILMELDSKTDILTHRFDERTAKGTHRLRLQVKDDRGNERNFVQTFVK"
     gene            812..1273
                     /locus_tag="PKM27_18720"
     CDS             812..1273
                     /locus_tag="PKM27_18720"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNR09361.1"
                     /translation="MARLKKGRPLPAFQSRLHDGSAISNRDLSSGKVILFFYPQDNTP
                     TCTEEACNLRDHYEDFIKQGYRVFGVSPDSIRKHQNFIKKYRLPYPLISDPALDLINK
                     FGVWGPKKLFGREYDGVLRTTFILRDGIVERVIDEVKAREHSEQILHSPLE"
     gene            complement(1289..2275)
                     /locus_tag="PKM27_18725"
     CDS             complement(1289..2275)
                     /locus_tag="PKM27_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNR09362.1"
                     /translation="MDKTSEINLTFEGVEPLELLGENNLKLNLLRKAYPDVQITSRGN
                     VLKLVGEKKNTQEVKAKFESMVRYLKEHQELTAHTVEDLLAGSNLFESRIQAYTEENI
                     ILHGREGRPIRAKTRNQKILVEASEQHDIVFAVGPAGTGKTYTAVALAVRALKNRLVK
                     KIVLTRPAVEAGESLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLAQFIESRIIEIA
                     PLAYMRGRTLDNAFIILDEAQNATPLQIKMFLTRLGPTAKCIITGDLSQIDLPRNQKS
                     GLAQAIYLLAHINGIAKVELTTEDVVRHRLVKEIIKAYSKDQNLERHQDEQH"
     gene            2370..3149
                     /locus_tag="PKM27_18730"
     CDS             2370..3149
                     /locus_tag="PKM27_18730"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNR09363.1"
                     /translation="MQWITLISDFGLADASAGRLKGAMLKQDPNLKFLDITHEIKPHD
                     IVTAGWVLKNSYRDAPEGSIHVISVLNNYNNTHSYLAFEFQGYYFIGPNNGIFSLAFE
                     KLPMDIFEYTDPAGNSFSVKEVLSHMVGHIISGKPLSELGPPVRNLIMRIQLQPVITR
                     HMIRGSVIYIDRYGNVVVNITRDQFEKTRAGREFSIYMKRNDTIHQISDNYQEVAVGE
                     TLCLFNSGNYLEIAIHAGNAAGMLGLSVDETIQIDFFDDKI"
     gene            3146..3445
                     /locus_tag="PKM27_18735"
     CDS             3146..3445
                     /locus_tag="PKM27_18735"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNR09364.1"
                     /translation="MITRIVKMVFHPEHTGRFLDLFGKHALNMQEVAGCLSLQLQQDA
                     NDPRIFFTLSAWQDLQSLEAYRQSELFRKIWTLVKPLFSERAQAWTTHTIYDGKN"
     gene            3432..4469
                     /gene="aroB"
                     /locus_tag="PKM27_18740"
     CDS             3432..4469
                     /gene="aroB"
                     /locus_tag="PKM27_18740"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNR09365.1"
                     /translation="MEKIDLKEYSIFLGNPAESLSSLLAQNPGVRLFVLTDHNTKFHC
                     LPLVEPLLRDRSWSVFSVAPGELNKNIHSCIHIWEAMTQAGLDRQAIMINLGGGIIGD
                     MGGFCASTYKRGIRFIQLPTTLLSQVDASIGGKLGIDFEGYKNHIGVFRDPLAVLIHP
                     DFLQTLSDRELKSGYAEVIKHCLIADGGHWQELSGIFDLRAASWQPIIRRSLEVKRKI
                     VSEDPFESGMRKLLNFGHTIGHALESHYLRTDHPLLHGEAIAAGMIYETLLSNRHTGL
                     TTDKKEAITGYIESLYGPPPAFDPDLLLPLMKQDKKNKEGKISFTLLRDIGESVWDVM
                     LTEEEMRLIKK"
     gene            complement(4756..5634)
                     /locus_tag="PKM27_18745"
     CDS             complement(4756..5634)
                     /locus_tag="PKM27_18745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNR09366.1"
                     /translation="MPPAPSPRIYLLTGAACISLSPILTKLTEVPGLTSAFYRVTLAM
                     VVLLPVVLYKKHYKLSPGKIGMALLCGLFFAMDLACWNMSLMISNTAVATVLGNLAPV
                     WTGLLLLIFRNQRPSAYYWVGVGITLCGVIYMIGWSRIIHFNLTAGNLLALAASFFYA
                     IYIVITRLTRSGISTMTFMFYSMLGYLLFSFLFAWLAGAPLSGFSSRSWLMLVLLAMV
                     PQLLGWLCVNHALGHLASTEVSMVLLGQIVIASLLATVVFDEILSLAQILGGLIVVLG
                     IAVTYLNRGFLKSVLE"
     gene            complement(5669..>6024)
                     /locus_tag="PKM27_18750"
     CDS             complement(5669..>6024)
                     /locus_tag="PKM27_18750"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR09367.1"
                     /translation="KNSASDSVRSLVRVWEHLSFNPHDKGGIRRYFDQATSETKRFEM
                     HVTTLNGGLRSHDPHTHKAEEIVLMIEGDTEMQIGDRFFKGRSGDVYFLGTQVPHAIR
                     NTGDKPCTYFAFQWQ"
BASE COUNT         1554 a         1573 c         1487 g         1410 t
ORIGIN      
        1 cggctgccgg gcaataaact ttcaatctac aaacaaaaaa taaacgatgg ctggatcccg
       61 cttacacagg gccaggggcg gaacgtgcac atcgacgttt acgattattc gggcaataag
      121 tcccaggtga aattttacct ccaaggcaat ggatccgccc cggaacaaaa aatcagagct
      181 tcccatccca tagcctatga tcagccgttt accgaagacc gggaagacct caaattgctg
      241 tttcccgcta attcccttta tacggacatt gacctgcaat tggatagatc ggtaaggaaa
      301 gagtcctggt cggcggttta ccgggttcac gacccgtcca ctcccctgca cacctcttat
      361 gtgatctcgc tcaaaccgga gggcataccc tcccggctaa aaaacaaatg cgtcatcggg
      421 ctttttaacg gccagtcggt gaccaattgc ggtggaacct ggaacggggc ttggttggag
      481 accaaaatca atgcttttgg ccagtttggg gtagtggtcg atactcttcc tccggtgatc
      541 accccggtgt ctttcaaaac cgacatgaaa agcagcagct tgatgaaatt caggatcacc
      601 gacaattttg aggtcgcggg ccaggccgac cgcctcactt ataatgcaac catcgatgac
      661 cagtggatcc tgatggaact ggattccaaa accgatatcc ttacgcaccg gtttgatgaa
      721 cggaccgcca aaggcacgca ccggctgcgc ctacaagtca aagatgaccg tggaaatgag
      781 cggaattttg ttcaaacctt cgtaaaataa aatggcccgt ttaaaaaaag gccgccccct
      841 tcctgccttc caaagccggt tgcacgatgg cagcgccatt tcaaaccggg acctttcttc
      901 cggtaaggtc atcctgtttt tttaccccca ggacaatacc cccacctgca ccgaggaagc
      961 ctgcaatctc agggatcatt acgaagactt tatcaaacag ggataccggg tatttggagt
     1021 cagccccgac tccatccgca aacaccagaa ttttattaaa aaataccggc ttccctatcc
     1081 cctgatctca gatcccgccc tggacctgat taataaattt ggagtatggg ggcctaaaaa
     1141 actgtttggc cgtgaatacg acggagtctt gcggactacc tttatcctcc gggacggcat
     1201 cgtggaaagg gtgatcgatg aagtgaaagc ccgggaacat tcggaacaga tcctccattc
     1261 acccttggaa taattcagag agccggtctc aatgttgttc atcctggtgc ctttccaggt
     1321 tttgatcttt gctgtaggct ttgatgattt cctttaccaa ccggtgcctt accacatctt
     1381 cggtggttaa ttccactttt gctatgccgt tgatatgggc gagcaggtaa atggcctggg
     1441 ccaggccgga tttctggttc cggggcaaat caatctggga taagtctccg gtgatgatgc
     1501 atttcgccgt tggcccaaga cgggtgagaa acatcttgat ctgcaggggg gtggcatttt
     1561 gtgcttcatc caggatgata aaagcattgt ccagggtgcg tccccgcata taggccaggg
     1621 gcgcgatctc aatgatcctg ctttcgatga attgggcaag cttgtccgcc gggatcatat
     1681 cgtcgagggc atcgtaaagc ggcctcagat aaggatcgat tttttccttc aggtccccgg
     1741 gtaaaaatcc aaggctctcg ccggcttcca ccgccggccg ggtgagtacg atttttttta
     1801 ccagcctgtt tttgagggcg cgaacggcaa gggcaaccgc cgtataggtt ttgcccgtcc
     1861 cggcaggccc gaccgcgaat acgatatcgt gttgttcgga ggcttccacg agtattttct
     1921 gattgcgtgt tttggcccgg atggggcggc cttcccgtcc atggaggatg atgttttcct
     1981 cggtataagc ctggatgcgg ctttcaaaaa ggtttgatcc cgccaacagg tcttcaacgg
     2041 tgtgcgcagt cagttcctgg tgttctttca ggtaccggac catcgattca aattttgctt
     2101 tgacttcctg ggtgtttttt ttctcaccga ccagcttaag cacatttcct ctcgatgtga
     2161 tctggacatc gggataggct ttacgaagaa gatttagttt caggttattt tcccctaaga
     2221 gttccagcgg ctccactccc tcaaacgtga ggttgatttc acttgttttg tccaatagtt
     2281 taatggatta aagatattaa agctttaaat aatatgtatt tttgaggcat tcttaattca
     2341 aaattacgta aaaacaggca ggcaagccca tgcagtggat tactttaatt tctgattttg
     2401 gcctcgcgga tgccagcgcc ggccggttga agggggccat gctgaagcaa gaccctaacc
     2461 tgaaatttct ggatatcact catgaaatca aaccccatga tatcgtcacg gccggctggg
     2521 tgttaaaaaa cagttaccgg gacgccccgg aaggttccat ccatgtcatc agcgtgctga
     2581 acaattacaa caacacccat tcttacctgg cttttgaatt ccaggggtat tattttatcg
     2641 gtcccaacaa cggcattttc agcctcgcct ttgagaaatt gcccatggac attttcgaat
     2701 ataccgaccc ggccggaaat tcattttcag tcaaggaggt gctcagccat atggtgggcc
     2761 atatcatctc cggcaaaccg ctgtccgaac tgggtccgcc cgtccggaac ctgatcatgc
     2821 ggatccagtt gcagcccgtg atcacccggc acatgatccg cggttcggtg atctacatcg
     2881 accggtatgg caatgtggtg gtgaatatta cccgcgacca gtttgaaaaa acccgcgcag
     2941 gccgggaatt cagcatttac atgaaacgca acgacaccat tcaccagatc agcgacaatt
     3001 accaggaggt ggccgtagga gaaaccttat gcctttttaa ttccggcaac tacctggaaa
     3061 tagccattca tgccggaaac gccgcgggca tgctcggcct gagcgtggat gaaaccattc
     3121 agattgactt tttcgacgac aagatatgat cacccggatt gtaaaaatgg tattccaccc
     3181 cgaacacacc ggccgtttcc tggatttgtt tggcaaacac gccctgaaca tgcaggaagt
     3241 ggccggttgc ctttcccttc aattgcagca agatgccaac gatcccagga ttttttttac
     3301 cctgagcgcc tggcaggacc tccaatccct ggaagcttac cggcaatccg aacttttcag
     3361 gaagatctgg acattggtaa aacccctgtt ttccgaacgg gcccaagcct ggaccaccca
     3421 cacgatctac gatggaaaaa attgacctta aggagtattc catttttttg ggaaacccag
     3481 cggaaagcct cagctccctg ttggctcaaa atccgggagt ccgccttttt gtcctgaccg
     3541 atcacaatac caaattccat tgtctccccc tggtcgagcc cctgttgaga gatcgctcgt
     3601 ggtcggtttt cagtgttgca ccgggagaat tgaataaaaa catccacagc tgcattcata
     3661 tttgggaagc catgacccag gccggccttg accgccaggc catcatgatc aacctcggag
     3721 gaggcataat cggggatatg ggtggatttt gtgcttccac ttataaaaga gggatccgtt
     3781 ttatccaatt gcccaccacc ctcctgagcc aggtggacgc ctccatcgga ggaaaactcg
     3841 gaatcgattt tgaaggctat aaaaatcata ttggggtgtt ccgggatccg ctcgccgtcc
     3901 tcatccatcc ggatttcctt caaaccctat ccgaccgtga acttaaaagc ggttacgccg
     3961 aagtgatcaa acattgcctt atcgcagacg ggggtcactg gcaggaactc tccgggatct
     4021 tcgatttgag ggcggcgtcc tggcagccca tcatccggag gtccctggaa gtcaaaagaa
     4081 aaatcgtttc ggaagatccc tttgaatccg ggatgcgcaa acttctcaat ttcggccaca
     4141 ccatcgggca cgccctcgaa agccattatc tgcgcaccga tcaccctctc ctgcatggtg
     4201 aagccatagc cgccggcatg atttatgaaa ccctgctttc caaccggcat accggcctta
     4261 ctacggataa aaaggaagcc atcaccgggt atattgaatc cctttacggc cctcctcctg
     4321 cttttgaccc ggacctcctc cttcctttga tgaaacagga caaaaaaaac aaggaaggaa
     4381 aaatcagttt caccctgctc cgggatatcg gggaaagtgt gtgggatgta atgctgacgg
     4441 aggaagaaat gagattgatc aagaaatagc tatcagcaat cagcaatcag ctatcagcta
     4501 tcggctatca gctatcggct atcagctatc ggctatcagc tatcagctat cagctatcgg
     4561 ctatcagcaa tcagctataa gctataagct ataagaataa aaggctgacg gctgaatgct
     4621 gaccgctgaa tgctgacggc tgaatgctga ccgctgaatg ctgaccgctg aatgctgacg
     4681 gccgaatgct gacggctgaa tgctgaccgc tgaatgctga ccgctgaatg ctgaccgctg
     4741 aatgctgaaa gctgactact ccaaaactga tttcaggaac ccacggttca gataagtgac
     4801 cgcgatcccc aacacgacga tcaagccgcc caggatctga gccaggctaa ggatttcatc
     4861 aaagacgacg gtggccagga ggctggcgat cacgatctgg cccagcaata ccatagacac
     4921 ttcggtcgag gcgaggtgtc caagggcatg gttgacgcaa agccagccca acaattgcgg
     4981 gaccatggcc agcaggacca acatcagcca ggagcgggag gaaaaaccgg aaaggggcgc
     5041 tcccgccagc caggcaaata aaaatgaaaa aagcagataa cccagcatgg aataaaacat
     5101 gaaggtcatg gtagaaattc cggaccgggt aagccgggtg atcacgatat aaattgcata
     5161 aaaaaaactg gctgcgagag ccaggagatt tcctgccgtc aggttaaagt gaataatcct
     5221 ggaccagcca atcatgtaaa tcactccgca aagggttatt cccaccccaa cccagtaata
     5281 ggccgaaggc cgttgatttc ggaagatcag caacaagagg ccggtccata ccggggccag
     5341 gttgcccaaa accgtcgcca ccgccgtatt ggaaatcatc agcgacatat tccaacaggc
     5401 caggtccatg gcaaaaaata aaccgcagag tagggccatt ccaatttttc ccggggacag
     5461 tttataatgc tttttatata aaaccaccgg caagagcacc accatggcca gagtcacccg
     5521 gtaaaaagca gaagtcaatc ccggcacttc cgtcaacttg gtcaaaatcg gcgaaaggga
     5581 tatgcaggcc gctccggtga gtaagtagat ccggggagaa ggggcaggag gcaagttggt
     5641 aagttagtaa tttggcaagt tagcaagttc attgccactg aaaagcaaaa tacgtacaag
     5701 gtttgtcccc ggtattccgg atggcatgag ggacctgggt gccgagaaaa taaacatccc
     5761 ccgatcttcc tttaaaaaaa cggtccccga tctgcatttc ggtatcgcct tcgatcatga
     5821 ggacgatttc ttcggcctta tgggtatggg gatcatggct gcgaagccct ccattcaggg
     5881 tggtcacatg catttcgaac cgtttggtct ctgaggtcgc ctggtcaaaa taacggcgga
     5941 tacccccctt gtcgtgggga ttgaaactca gatgctccca aacgcgcacc agggaacgaa
     6001 ccgaatcgga ggcggaattt ttca
//
LOCUS       DAOQXT010000180         5980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_13553_length_5980_cov_7.098059, whole genome shotgun sequence.
ACCESSION   DAOQXT010000180 DAOQXT010000000
VERSION     DAOQXT010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13553_length_5980_cov_7.098059"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            706..1074
                     /locus_tag="PKM27_18755"
     CDS             706..1074
                     /locus_tag="PKM27_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNR09368.1"
                     /translation="MKSLYTILLLLASNAFMTMAWYGHLKYSEWKGMHKMTLISIILL
                     SWGIAFFEYCLMVPANRIGYKANGGPFTLVQLKVIQEVITLLVFSVFTVILFKNESLK
                     WNHGVAFLLLVLSVYFVFRK"
     gene            1144..1497
                     /locus_tag="PKM27_18760"
     CDS             1144..1497
                     /locus_tag="PKM27_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09369.1"
                     /translation="MDDDWQFDFDWLQTRHRIKDTLRVEKLPDLNAILLFIGVHELGR
                     WKSTKFKKEEKQDLMHVAVCTLLSRDGYYEWDGLDSDGWPHFKPLRVFQEKGLETQEK
                     YLKKKIIQYFNEQSS"
     gene            1502..2146
                     /locus_tag="PKM27_18765"
     CDS             1502..2146
                     /locus_tag="PKM27_18765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR09370.1"
                     /translation="MNLKSYNPIILSILSLFLFACAQSGPQRAIIETDMGEIEIELYD
                     QTPQHRDNFIKLVKSNFYDDLLFHRVMPNFVIQGGDPESRNAPSETLLGGGGPGYQIK
                     AEIGAPHFRGTLAAARNQNPEKMSSGSQFYIVLGNKLTEQELDQWERTKGIKYNPAQR
                     AKYLAEGGLPSLDGDYTVFGEVTRGMEVAVKIMNQPTNQANRPLQDVKMKIRLK"
     gene            2228..2827
                     /locus_tag="PKM27_18770"
     CDS             2228..2827
                     /locus_tag="PKM27_18770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR09371.1"
                     /translation="MSTAQDTIQIQVETPLGTMKGFLYNETPLHRDNFSKLAGEGYYD
                     SILFHRVIPGFMIQGGDPDSKNAKPGAALGMGGPGYQVPAEFVDSLAHVKGAIAAARN
                     NNPEKKSSGSQFYIVSGRPVAPTELTLQEFKFRFKYPESVKKEYAALGGASFLDQDYT
                     VFGRITEGLDIIDKIAAAPADSRNRPLENIWMKITIIAK"
     gene            2824..3582
                     /locus_tag="PKM27_18775"
     CDS             2824..3582
                     /locus_tag="PKM27_18775"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR09372.1"
                     /translation="MTNAVKNKIILGIDIGGTGIKAGLVDISNGSLQSEVLKVKTPRP
                     SNPENVLKAVREVITAMNYKGPVGCGFPAIVHHAVVQNAANIDKKWIGTNSEKLFNRI
                     RGCKFHVANDADVAGMAEMHFGLGKGKKGTVIFITIGTGLGSALFRDGVLIPNTEFGH
                     LNYREADYEFYASNTAREKHKMSWKAWGSQFNHFIEHLRFIFSPDLIILGGGLSKRWV
                     DYKGQITSKVKVIPARLQNHAGIIGAAVFAQSMD"
     gene            3621..4268
                     /locus_tag="PKM27_18780"
     CDS             3621..4268
                     /locus_tag="PKM27_18780"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNR09373.1"
                     /translation="MKSAALFLGFSLVLLFACKSDSSSTASPQNGTDPLKTTTSSADV
                     NDFEKLDHNFNITPEGSRLFFKGFFQNQLTDGAMTINKGTLHIAGGLVSEANLNIDFR
                     TIEMIANRNEGREAFMKGSSAFDMGKYPNGNFTLEECLKAVNDQDATHIFKGKLQMKG
                     KTIPVNVRVRIDYRPKYITILSDQIVIKASDLGIKVADPSQENIYFSLTINGEIF"
     gene            4252..5799
                     /locus_tag="PKM27_18785"
     CDS             4252..5799
                     /locus_tag="PKM27_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNR09374.1"
                     /translation="MGKFFKGTVVLNKKLGVKKNNPAYSTGLEWGGFSSTKILSLSPV
                     DGKGIASVYQTTEADYETVVRQATQAQKTWQTWPAPKRGEVVRQLGDAFRSHKEALGQ
                     LVSYEMGKSLQEGLGEVQEIIDICDFATGLSRQLYGLTLPSERPAHRMFEQWLPLGVV
                     GIISAFNFPVAVWSWNAMIALVCGNSTIWKPSEKTPLCAVAVMHIVRETLRSNRVPEG
                     VLCMIQGDRTAGEWISRDPRISLVSATGSVRMGREVAREVASRLGKALLELGGNNAII
                     ITPTANLKIVLAGTVFGAVGTAGQRCTSTRRLIIHESIYDQVVQALIKAYKQLKIGNP
                     LDPSNHCGPLIDAGAVHAYLAALEKIRQSGGKLLTPSGSLKGKGFESGCYVRPCIAAV
                     VPSMEIVQTETFAPILYVMKYKTLEEAIAIQNGVPQGLSSAIMTNELREAEMFLSAQG
                     SDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTSTINYSESLPLA
                     QGIRFEV"
BASE COUNT         1546 a         1502 c         1526 g         1406 t
ORIGIN      
        1 attactaaaa tttttttgta actatgtctg aaaatttagg gggctgtttc aggggcctgc
       61 cccgggcagt atttgagtat tggcagtcgg ggggacaggg gtgttgaaga tattttatat
      121 gaaattcctt ttttgtagta gttctccaga aattttaata gaccatttct gtgctccttc
      181 ccaagggcaa ggaaaatggt tctatttagg tatactgcca ggatccttgg ttcccccgtg
      241 ggcctgcccc caggagccat ttgagaattg gtagtcaggg gggacagggg tgttgaagag
      301 gtttatatga attttctttt ttagtaatta ttctccggcc cctgtcccct caaggggaac
      361 ctaacccttg gcgttccgaa ataaatttaa ttcacccatc tctgtacata ttccggcatg
      421 ggcgaggaag attgttttag ttgagtacat cgccaggctc cttggttccc ctgcaggctt
      481 gccccaattg tgatatttga aaactagtac tcgggggact gccccaatag tgatatttga
      541 aaattggtag tcacgggagg acaggggtgt tgaagatatt ttatatgaaa ttcctgtttc
      601 ttattgtatt ccgacccgca ggcgcgggat tgacatgtac cacataactc accccccatt
      661 tcagaatacc acttatttcc taattttccg ccactatttg aatctatgaa atcgctttac
      721 accatcctcc tcttactggc cagcaatgca tttatgacca tggcctggta cgggcatttg
      781 aaatacagcg aatggaaagg catgcacaaa atgaccctga tctccatcat cctcctgagc
      841 tggggcattg ccttttttga atattgcctc atggtgcctg ccaaccgcat cggatacaaa
      901 gcaaacggag gcccgttcac cctggtccag ctcaaggtca tccaggaagt gattacgctt
      961 ctggtgtttt cggtcttcac cgtaatcctt ttcaaaaatg aatccctgaa atggaatcac
     1021 ggagtggcct tcctcctcct ggtcctgtcc gtgtattttg tctttcgcaa gtaatgcgtg
     1081 accggggcat ttacgattcc gcggggaagc ccgcgattgc tttcatatcc ttatttttgc
     1141 gtgatggatg atgactggca gtttgatttt gattggttgc agaccaggca ccggatcaaa
     1201 gatacgctcc gggtcgaaaa acttcctgac ctcaatgcca ttttgctttt tattggggtt
     1261 catgaactgg gccgttggaa atccaccaaa tttaaaaaag aggaaaaaca ggacctgatg
     1321 catgtcgcgg tctgcaccct gttgagcagg gacggttatt acgaatggga tggcctggac
     1381 agcgacggct ggccccattt caagcccctt cgggtattcc aggaaaaagg cctggagacc
     1441 caggaaaaat acctgaagaa aaaaatcatc caatatttta acgaacaatc ctcctgatct
     1501 aatgaactta aaatcttaca atccgataat cctgtcaatc ctatcccttt tcctatttgc
     1561 ctgtgcccaa tccggtcctc aacgggccat cattgaaacc gatatgggcg agatagagat
     1621 tgaactttac gatcagaccc cccagcaccg ggacaatttt ataaaactgg tgaaatccaa
     1681 tttttacgac gacctgctgt ttcaccgggt gatgcccaat tttgtgattc agggcggtga
     1741 cccggaatcc cgcaacgccc cttcggaaac cctcctgggc ggaggaggcc cgggttacca
     1801 gatcaaagcg gaaatcgggg ccccgcattt caggggaacg cttgcggccg cccgtaatca
     1861 aaacccggaa aaaatgtcct ccggttccca gttctatatt gtactgggca acaaactgac
     1921 cgaacaggaa ctggatcaat gggagcgtac caaaggaatc aaatacaacc cggcgcagcg
     1981 agccaaatac ctggcagaag ggggactgcc ctccctggat ggagactata ccgtttttgg
     2041 ggaggtcacc cgcggcatgg aggtggcggt aaaaatcatg aaccagccga ccaaccaggc
     2101 aaaccgcccc ctccaggatg tcaagatgaa aatacgtctc aaataatgat ttatcccgat
     2161 gatttccatc tcccgataat ccttgtacat gaaacagatc ttcttaattt taatcaccgt
     2221 cattaatatg agtacagcac aggatacgat tcaaatccag gtagaaaccc cgctggggac
     2281 catgaaaggt tttctttaca atgaaacgcc cttgcaccgg gacaatttca gcaaactggc
     2341 cggagaaggg tattacgaca gcatcctgtt ccaccgggtg attcccggat ttatgatcca
     2401 gggaggcgat cccgattcaa aaaatgcaaa accgggcgcc gcgctgggaa tgggaggacc
     2461 gggttaccag gtcccggccg agttcgtgga ttccctggcc catgtcaagg gggccattgc
     2521 cgcggcacgc aataacaacc cggagaaaaa atcttccggc tcccagttct atatcgtttc
     2581 tgggaggccg gtcgccccga ccgaattgac cctccaggag ttcaaattcc gttttaaata
     2641 tccggagagt gtgaaaaaag aatatgctgc cctgggcggc gcttcctttc tggaccagga
     2701 ttatacggtc tttggccgga tcaccgaggg cctggacatc attgataaaa tagcagccgc
     2761 accggcagac agccgaaacc ggccgcttga aaacatctgg atgaaaatta caattattgc
     2821 caaatgacca acgcagtaaa aaataaaatc atactcggca tcgacatcgg aggcaccggc
     2881 atcaaggcgg gcctcgtgga catctccaac ggaagtctgc agagcgaagt gctaaaggtg
     2941 aaaacacccc ggccttccaa ccctgaaaac gtgctgaaag ccgtgcggga agtgatcacc
     3001 gccatgaatt ataaagggcc ggtaggttgc ggattcccgg ccattgtcca ccatgccgtc
     3061 gttcaaaatg ccgccaacat cgataaaaaa tggatcggca ccaattccga aaagctgttt
     3121 aaccggatcc gcggatgtaa attccacgtg gccaacgacg ccgatgtggc aggcatggcg
     3181 gaaatgcatt tcggactggg caaagggaag aaaggtaccg tgatcttcat caccattggg
     3241 acagggctcg gttctgcctt gttccgcgac ggagtgctga tccccaatac ggaattcggc
     3301 cacctcaact accgggaagc ggattatgaa ttttatgcct ccaacacggc ccgtgaaaaa
     3361 cataaaatga gctggaaagc ctggggctcg cagtttaatc attttatcga acacctccgg
     3421 ttcatctttt ccccggacct gatcatcctg ggcgggggat tgagtaaacg atgggtggat
     3481 tacaaaggcc agatcacctc gaaggttaaa gtgatcccgg ccaggctgca aaaccacgcc
     3541 ggcatcatcg gtgcggcggt ctttgcacaa agcatggatt gaaggatttt cctatttttg
     3601 catcctcaaa ttttccattc atgaaatcag cagctttatt cctgggattt tccttggtcc
     3661 ttttgtttgc ctgcaagagc gattcttctt cgacggcgtc tccccaaaac ggcaccgacc
     3721 cgttaaaaac cacgaccagc agcgcagacg tcaatgattt tgaaaaactc gaccataatt
     3781 tcaacatcac tccggaagga agccggctgt tttttaaggg ctttttccaa aaccagctca
     3841 cagacggagc catgaccatt aataaaggca cgctccatat cgccggcggc ctggtctccg
     3901 aggccaattt gaatatcgat ttccggacca tcgaaatgat tgccaaccgc aatgaaggac
     3961 gtgaagcctt tatgaaaggc agttccgcct tcgatatggg aaaatatccc aatggcaatt
     4021 ttacactgga agaatgcctc aaagctgtca atgaccagga tgcaacccat atttttaaag
     4081 gcaaactgca aatgaaggga aagaccattc ccgtgaatgt gagggtgagg atcgattacc
     4141 ggccgaaata catcaccatc ctttccgacc agatcgtgat caaggcgtca gatctgggaa
     4201 tcaaggtcgc cgatccatcc caggaaaata tttatttttc cctgacgatt aatggggaaa
     4261 ttttttaagg gtaccgtggt attaaataaa aaactcggag taaaaaaaaa caatccggca
     4321 tactctaccg gcctggaatg ggggggcttc agcagtacta aaatcctctc cctctcccca
     4381 gtggacggta aggggatcgc ctccgtttat cagaccaccg aggccgatta tgagaccgtg
     4441 gtcaggcagg ccactcaggc acaaaaaaca tggcagacct ggcctgctcc caaaagaggt
     4501 gaggtcgtca ggcaattggg ggatgccttt cgcagccata aagaagccct cggccagctg
     4561 gtttcttatg agatggggaa aagcctgcag gaaggcctcg gagaagtgca ggagatcatt
     4621 gatatctgtg attttgcgac cgggctttcc cgccagctct acgggctgac cttgcccagc
     4681 gagcgccctg cgcaccggat gtttgaacag tggctccccc tgggtgtggt gggcatcatt
     4741 tccgccttta actttcccgt ggccgtctgg agctggaatg ccatgatcgc cctggtatgc
     4801 ggcaattcca ccatctggaa acccagtgaa aaaaccccat tgtgcgcagt ggccgtcatg
     4861 cacattgtcc gggagacgct ccggtccaac cgggttccgg aaggagtcct ctgcatgatc
     4921 cagggcgacc gcaccgccgg agaatggatc agccgggatc cccggatttc tctggtgagc
     4981 gccaccggga gcgtacggat gggcagggaa gtggcccggg aagtggcttc cagactgggt
     5041 aaggccttgc ttgaactggg cgggaataat gccatcatca ttaccccgac cgccaatctt
     5101 aaaatagtac tcgcaggtac cgtgtttgga gccgtaggca ctgccgggca acgatgcact
     5161 tccacccggc ggctgatcat tcatgaaagc atttatgacc aagtggttca agccctgatc
     5221 aaggcctaca agcaactgaa gatcggtaat ccgctcgacc catccaacca ctgcggccct
     5281 ctgattgatg caggagcggt ccatgcctac ctggcggccc ttgaaaaaat caggcagtcc
     5341 ggcggaaagc ttctgacccc ttccgggtcg ttgaagggca aagggtttga aagcggctgt
     5401 tatgtcaggc catgcatcgc ggccgtggtt ccgtccatgg aaatcgtgca gacggagact
     5461 ttcgctccca tcctgtatgt aatgaagtat aaaacgctcg aagaagctat agccattcag
     5521 aacggggtgc ctcagggact ctcctccgcc atcatgacca atgaactccg ggaagcggag
     5581 atgtttttat ctgcccaggg ttccgattgc ggtatcgcca atgtgaacat cgggaccagc
     5641 ggtgcggaaa tcggaggcgc attcgggggc gaaaaagaga ccgggggagg ccgggaaagc
     5701 ggttccgatg catggaaagc ctatatgcga aggcagacca gtaccatcaa ttactcggaa
     5761 tcattgcccc tggcgcaggg gatcaggttt gaggtttgag gattgaggtt tgaggtttga
     5821 gggagctggg agctgggagc tggaagttgg gagccagggg agggtctaca acaaatttta
     5881 aagaaaaaga atttactaaa ctgctaagcc agtgcgtccc tagcttctag tgccgtaaca
     5941 actaatattt gctaataatt attaaagatt gtaatttgag
//
LOCUS       DAOQXT010000181         5923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_13739_length_5923_cov_6.709441, whole genome shotgun sequence.
ACCESSION   DAOQXT010000181 DAOQXT010000000
VERSION     DAOQXT010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13739_length_5923_cov_6.709441"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKM27_18790"
     CDS             <1..442
                     /locus_tag="PKM27_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR09375.1"
                     /translation="LIYRGHNVMKGYYKKPEASAEALRNGWMHSGDVAVRDQDGFYFI
                     VDRTKDMIIRGGFNVYPREVEELLLHHPAVSMAAVIGVPDERLGEEIQACVVLKTGTR
                     VMEEELIQWARERISDYKFPRSIRFMTALPMSATGKILKKDIPR"
     gene            complement(503..2221)
                     /locus_tag="PKM27_18795"
     CDS             complement(503..2221)
                     /locus_tag="PKM27_18795"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNR09376.1"
                     /translation="MIKASLSSCLLAAVFFLAIHACKSGSGIDTRGIARDPESINQGM
                     AIFNMQCSPCHSFRQDGIGPQLGGLTLERETEWIKKFILNPAALFASGDVTAKQLVEK
                     YKVIMPAFALKPEELDDLLAFMHTHPKPSFADTSGLVALENPIPDTIRHSDLVVDLEL
                     VTRFPASSDSGKLPLTRITKMDPHPNTGESYVLDLRGKLYRLEKNKPVVYMDMAKFRP
                     KFINQPGLATGFGSFAFHPEFHHNGLLYTTHTEPPGTATADFAFPDSIRVLLQWVLME
                     WKTPAPGEKEFRGSGRELFRVNMESGIHGVQEIIFNPLSKPGYEDYGMLYIGIGDGGA
                     VEHGYPFIAHNLKTVWGNILRIDPAGNNSRNGKYGIPASNPFAKNPDALGEIFATGFR
                     NPHRITWSRTGQMLVSNVGHYNIESLNLVEAGLDFGWPLTEGRFLVNAYADLKKVYAL
                     PPDHGMPLTYPVVAYDHDEGKAITGGYEYTGEAVPPLKGKFLFGDIPTGRLFYVNMAD
                     IQSGSEATIKEWSVAHQGKVQSLRELCGTDRVDLHFGRDAAGEIYIMTKPDGKLYKLV
                     KAEEIN"
     gene            2504..3895
                     /locus_tag="PKM27_18800"
     CDS             2504..3895
                     /locus_tag="PKM27_18800"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HNR09377.1"
                     /translation="MHDADKNPRRDFLQKLSLGGLSLGTMTMAPIEERLEYTTQKVNR
                     YSAPSDLKITDMRIAEISGVVFRTPIVRIYTNQGIIGHGDVRDGAAKEYALFLKSRLL
                     GENPCNVERLFKIISQFGHHARQGGGVSGVEMALWDIVGKAYNVPVYQLLGGKYRDQV
                     RVYCDTTTSRDPQEYAKRMQERVDKGYTALKMDIGIGLLRDIPGTVINAPDGKLNPYQ
                     YEYRKYNMTKHPFTGVQVTDKGIEKLCEFVEVMRNQIGYEIPMGTDHWGHFGLNEAIK
                     ITRAMEKYNLAYTEDLIPWQYTDQWREITQSTTTPTQTGEDIYMLEGGFKEIIDKHAV
                     DIVHPDPNTAGGILETKRIGDYASLHGIGFMHHHAAGPVSFLGSVHSAAATENFLWLE
                     HHAVDSPKFEDLVTGIEKPFVKNGYVKVPEKPGIGVELNEDVVKEFIIKGQQMFAPTP
                     EWNEIRAWDRLWS"
     gene            complement(4048..5580)
                     /locus_tag="PKM27_18805"
     CDS             complement(4048..5580)
                     /locus_tag="PKM27_18805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR09378.1"
                     /translation="MHYTVKSFLFILFSSVLVSLLESSCKNVEPKTELTLDSARVALQ
                     HLLARADSLELDTPYEKVPGDALSHQAAGFAKIMCSAVFISKLDPEFAAANLGGFVCS
                     FEERKNFDIPRVDYQKKSVSVFLPDRSVRTAVYLGDQSQGCICLPQGEDKLHFNPTKV
                     VPNLPEASKTPWPMGDLVAEEPWPAEVDKSKIDQAVQAAFEGDGMTAAFVVTYKGMII
                     AEKYGEHITRTTPLESWSMGKSLTATLMGILIQAGDYALDQAAPIPEWQKQGDERSKI
                     KIQDILHMSSGLRFRAPQDPDYEASLGYPDHLYVYTGGINSFTWAATRPQQWPPNSVG
                     RYRNSDPLLINYLIRLGVEKKGIDYHRFPQGALFDKIGIRTMVMETDPYGNFLTQGYE
                     LASARDWIRLGNLYLQDGVWNDERILPEGFVKFVSTLAEPWVKDGRPIYGGFFWINGD
                     GKRPIPKEAYMMLGAGGQSTTIIPSHGLVIVRMGHYRGASAGQVSLDNAYKLILEAVP
                     AL"
     gene            complement(5681..>5923)
                     /locus_tag="PKM27_18810"
     CDS             complement(5681..>5923)
                     /locus_tag="PKM27_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09379.1"
                     /translation="GLAEFYRDDDLLMMKINGQIMEALTYKGDNTFEGGLAMVRAKFA
                     LSPKGASVEISTGDFDSGDLSRVKVSKGEKILKYGG"
BASE COUNT         1510 a         1517 c         1463 g         1433 t
ORIGIN      
        1 actgatctac cgcggacaca atgtgatgaa aggatattat aaaaaaccgg aagccagcgc
       61 ggaagccctg cgcaatggct ggatgcatag cggggatgtg gcggtcaggg accaggatgg
      121 tttttatttc atcgtggacc gcaccaagga tatgatcata cgcgggggtt ttaatgttta
      181 tccgagagag gtagaggagt tattgctcca ccaccctgct gtatccatgg ccgctgtgat
      241 cggtgtaccc gatgaaaggc tgggtgaaga aatacaggcc tgtgtagttc tgaagacagg
      301 aacgcgggtc atggaggaag aattgatcca atgggccagg gaaaggatct cagactataa
      361 attccctcgt tcgattcggt ttatgacagc attgcctatg agtgctacgg ggaaaatttt
      421 gaagaaggat attccaagat gattccacct gctatgcaag tgagtacctt cattcttaat
      481 ttccttaatt ccttttattt ccttaattta tttcttccgc ttttacaagt ttataaagtt
      541 tcccgtccgg tttggtcatg atataaattt ctccggcggc atcccgcccg aaatggaggt
      601 ccacccggtc cgtcccgcag agctcccgca agctctgaac cttgccctga tgggctactg
      661 accattcttt gatcgtcgct tcgcttcccg attggatatc agccatattc acataaaaca
      721 aacggcctgt gggaatatct ccaaataaaa attttccctt gaggggaggt acggcttctc
      781 cggtatattc gtatcccccg gtgatggcct tgccttcatc gtggtcatag gccaccaccg
      841 gataggtgag cggcatacca tggtccgggg gaagggcata caccttttta aggtccgcgt
      901 aggcattgac cagaaaccgg ccttccgtga ggggccagcc aaaatccaat ccggcttcca
      961 cgagattgag ggattcaata ttatagtggc ctacattgct caccagcatc tgtccggtcc
     1021 ggctccaggt gatccggtgc ggattgcgaa agccggtggc gaaaatctct ccgagggcat
     1081 ccgggttctt tgcaaaaggg ttggaagcgg ggatcccgta tttcccattc ctgctgttgt
     1141 tgcctgcagg atcgatgcgc aaaatatttc cccataccgt tttgagatta tgggcaatga
     1201 aaggatatcc atgttccacc gctcccccat cgccgatccc gatataaagc attccataat
     1261 cttcgtaccc gggttttgaa aggggattga aaatgatctc ctgtaccccg tgaatgccgc
     1321 tttccatatt aacccgaaac aactccctgc cggaacctct gaactccttc tccccgggtg
     1381 caggcgtctt ccattccatc aggacccatt gcaacaaaac gcggattgaa tccggaaaag
     1441 cgaaatctgc cgtcgccgta cccggaggtt cggtatgagt tgtgtacaga agcccgttat
     1501 gatgaaactc cggatgaaaa gcaaaacttc cgaatcccgt agccaggccc ggctggttga
     1561 tgaacttcgg ccggaatttg gccatatcca tatagaccac cggtttattt ttttccaggc
     1621 gatagagttt ccccctgaga tccaggacat acgattcacc ggtgttcggg tggggatcca
     1681 tcttggtgat cctcgtaaga ggcagtttac cgctgtcact cgaagccgga aaccgggtga
     1741 ccagctcgag gtcaacgacc aggtccgaat gcctgatggt atccgggatg ggattttcca
     1801 gagcgaccag gccgctggta tctgcgaaac ttggctttgg atgagtatgc ataaatgcca
     1861 ggaggtcatc gagttcttcc ggtttgaggg caaaggcagg catgatgact ttgtatttct
     1921 ccaccagttg ttttgcagta acatctcccg aagcaaacag ggctgccgga ttcaggataa
     1981 attttttaat ccattcggtt tcacgctcaa gggttaggcc tcccagttgt ggaccaattc
     2041 cgtcctgcct gaaactgtgg cagggactgc actgcatatt aaagatggcc attccctggt
     2101 ttatgctttc agggtccctg gcgatcccgc gagtgtcgat cccggaaccg gatttgcagg
     2161 catgaatggc aaggaaaaaa acagcggcga gaaggcagct gctcagcgaa gctttaatca
     2221 tacggaaaga ggtcaaaaag tctacccaaa gctaattctt tcgtccaagg cattctccct
     2281 gaaatccaat aaaattcgga tacgctcccg gaataagggc attgttgggg gttcgaaatt
     2341 aaaatcagac taggaataaa acggtaatcc ccaatcaatt caacaattcg acaattcgac
     2401 atttcaacat ttcttagaat cggtcccaca atcacgaaat tggtgttaca tttgaaaaaa
     2461 acaatgttta aactttatta ttcactcaaa aagatatcaa accatgcatg atgctgacaa
     2521 aaatccacgc agagattttc tgcagaaatt gagcctgggc ggactgagcc tgggcacgat
     2581 gactatggca cccatcgaag aacgtttgga atacacgacg cagaaggtaa accggtattc
     2641 cgccccttcc gacctgaaaa tcaccgatat gcgcatcgcc gaaatcagcg gggtggtatt
     2701 caggaccccg attgtccgca tctataccaa ccagggcatc atcggccatg gagacgtgcg
     2761 cgacggggcg gcaaaggaat acgctttgtt cctcaagagc aggctgctgg gtgaaaaccc
     2821 ctgcaatgtc gagcgcttgt ttaaaatcat cagccagttt ggccatcacg cacgccaggg
     2881 aggcggggtc tcgggagtgg aaatggcgct ctgggacatc gtaggcaagg cctacaacgt
     2941 accggtttat cagttgctcg gggggaaata ccgggaccag gtcagggtct attgcgatac
     3001 gacgacatcc agggatcccc aggagtatgc caaacgcatg caggagcggg tcgacaaggg
     3061 ttataccgcc ctcaaaatgg acatcggcat cggcctgctg cgcgacatcc cggggactgt
     3121 gatcaatgct ccggacggaa aactgaaccc ctatcaatat gagtaccgga aatacaatat
     3181 gaccaaacat ccatttaccg gagtccaggt gaccgacaaa ggaattgaaa aactttgtga
     3241 atttgtggaa gtcatgcgca accagatcgg ttatgaaatc cccatgggta cagaccattg
     3301 gggccatttc ggattgaacg aagccatcaa gatcacccgg gccatggaaa aatacaacct
     3361 ggcctataca gaggacctca tcccctggca atacaccgac cagtggaggg aaatcaccca
     3421 gtcgacgacc acgcccacgc agaccggcga agacatctat atgctggaag gaggatttaa
     3481 ggaaatcatc gacaaacatg ccgtcgatat tgtccatccc gaccccaaca ctgccggagg
     3541 aatcctggaa accaagcgga taggcgatta tgcttccctg catgggatcg gttttatgca
     3601 ccaccatgcg gcaggacccg tctccttttt gggatccgtc cacagcgcag cagccacgga
     3661 aaatttcctt tggctggaac accatgccgt agatagtccc aagtttgaag acctggtgac
     3721 cgggattgaa aagcctttcg tgaaaaacgg gtatgtcaag gtgccggaaa aacccggaat
     3781 tggcgttgaa ctcaacgagg acgtggtgaa ggaattcatc atcaaaggtc agcagatgtt
     3841 cgccccgacg cccgaatgga acgagatcag ggcttgggac cggctttgga gttgatcttt
     3901 attatacgtt ccgcttatct ggattttaac ttgcctggaa ataaaaccgc tgttataaat
     3961 taacggcggt ttttttattc cttgaatcct gagaattcag caggattaat cctgaatgat
     4021 tccgttcagg acgaataaat tcgtccgtta aagagcgggt accgcctcca aaatcaattt
     4081 atacgcatta tccagggata cctgacctgc actcgcacct ctgtaatggc ccatgcggac
     4141 aatgaccaga ccatgcgaag ggatgatggt agtggattgc ccacctgcgc cgagcatcat
     4201 ataagcctct ttgggtatgg gccgttttcc atccccgttg atccagaaga aaccgccata
     4261 gatgggtctg ccatccttta cccagggttc tgccagggtg cttacaaact ttacaaatcc
     4321 ttccggcagg atccgttcgt cattccatac cccatcttgt aagtaaagat tacccagtct
     4381 gatccagtcc ctcgccgaag ccagttcata accctgggtc aggaaattgc catagggatc
     4441 agtctccatc accatcgtgc ggatgccgat tttatcaaag agggctcctt gggggaaacg
     4501 gtgatagtcg atcccttttt tctccactcc aagccggatc aggtaattga tcagcagcgg
     4561 atcgctgttg cggtaccggc ccacgctgtt tggcggccat tgttgcggcc gcgttgccgc
     4621 ccaggtaaaa gaattgatgc ctccggtata aacataaaga tgatcgggat aacccaggga
     4681 ggcctcgtaa tcaggatcct gcggcgcccg gaaacggagc ccgctggaca tgtgaaggat
     4741 gtcctggatt ttgatttttg acctttcgtc tccctgcttc tgccattccg gtattggagc
     4801 cgcctggtcc agggcgtagt cacccgcttg aatcagaata cccatgagcg tcgcggtcag
     4861 gcttttcccc atcgaccagc tttcaagcgg cgtcgttcgg gtaatatgct ccccatattt
     4921 ttctgcgatg atcattccct tgtaggtgac cacaaaggcg gcggtcatgc catccccttc
     4981 aaaagcagcc tgcacagcct gatcgatctt ggatttgtcc acttctgcag gccagggctc
     5041 ttctgctacc agatcaccca tgggccatgg ggtctttgac gcttcaggca gattgggcac
     5101 caccttcgtg ggattgaaat ggagtttgtc ctccccttgt ggcaggcaaa tacatccctg
     5161 gctttggtct ccaagataaa cagccgtgcg tactgaacgg tccggcaaaa agacagagac
     5221 cgatttcttt tgataatcaa ccctggggat atcaaaattt tttctttcct caaaagaaca
     5281 gacaaatccc ccaaggttgg ccgcggcaaa ttccggatcc agcttcgaaa taaaaactgc
     5341 tgaacacata attttggcaa atcccgcagc ctgatgggat aaagcatcac cgggtacttt
     5401 ttcgtaaggg gtatccaatt caagggaatc ggctcttgcc agcaaatgct ggagagcgac
     5461 ccgggccgaa tccagagtca gctctgtttt tggttcaaca tttttacaag aggattccag
     5521 gagcgaaacc aacacactgg aaaataggat gaacagaaat gactttaccg tataatgcat
     5581 gatgtgatta cattaatttg aaaatggcca ccgcagtcca aatactgatt acagactatt
     5641 gactattgac tattgactac tgattactga ctactggcta ctatcccccg tacttcaaaa
     5701 tcttctcccc tttgctcacc ttcaccctgg acaagtctcc ggaatcaaaa tcacccgtcg
     5761 aaatttcgac cgaggcacct ttggggctca gggcaaattt tgctctgacc attgccaggc
     5821 ctccttcaaa agtattgtcc cctttatagg tcagggcctc catgatctgc ccattgatct
     5881 tcatcatcag caggtcgtcg tcccggtaga attcggccag tcc
//
LOCUS       DAOQXT010000182         5836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_13998_length_5836_cov_5.686040, whole genome shotgun sequence.
ACCESSION   DAOQXT010000182 DAOQXT010000000
VERSION     DAOQXT010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13998_length_5836_cov_5.686040"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKM27_18815"
     CDS             complement(<1..181)
                     /locus_tag="PKM27_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNR09380.1"
                     /translation="MDNISCIRQQADIKQINRCKDRVSELNGTFDYLSQGLELAGNNV
                     RLKILYLLYEEKQLCV"
     gene            complement(462..1433)
                     /locus_tag="PKM27_18820"
     CDS             complement(462..1433)
                     /locus_tag="PKM27_18820"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNR09381.1"
                     /translation="MKIFIFPIETRGQKHIGIRTEHFDPACNALIKKIPRSHWNPGTK
                     SWCIPYEKEVWRRCQESLAAFEIVKTDTRAPADKSAERNLPSDLQAEFDTYYTQLYVQ
                     RYSLNTIRTYCSSFKYFLYQCRSMHPKDWTLEDFKRWLRSEIEKNKWSEAYQNTIINA
                     LKFYFEKIRNEARAFWEVRPRRPIKLPGTLSLDEVNRLLNSCENLKHRTMLTLIYACG
                     LRISELIKLRKADIDRGQKRILIKAGKGKKDRYVILPDRMALLMQKYLEIYPVRYGLL
                     KARTEAPTVPGVCKPSFTGHCNVQELMHTLQCTPSGTPTPPICLKQE"
     gene            complement(1718..3139)
                     /locus_tag="PKM27_18825"
     CDS             complement(1718..3139)
                     /locus_tag="PKM27_18825"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNR09382.1"
                     /translation="MELTRREFIGKMRLATLAGLSTGVSGLPFLAGCKTGRHSLPSTA
                     DSVILLWMAGGMAHTETFDPKKYSPFVKGMDSASVFSTFPSIPTRLDGIRFSEGLERI
                     ADTMHLGTLIRSYRAADLGFILHTRHQYHWHTCYPPPQSIQPPHIGAWIAKELGPKQE
                     VMPAFIDIGQRFTVGEGEELKAFHSAGFLGSEFGPFSIPDPVNGLESISPPAGMDLAH
                     FERRQKLYRELVKAGPLANAHEYQQESLMRSMERSYALLKSPEAKAFDLHEEPKEIYD
                     QYNTGRFGLGCLMARRLVERGARFISVTTEYEPFLGWDTHENGHTRALEMKKQVDQPI
                     HQLITELHERGLLDRTLVILASEFSRDMLIEGRPDQKVLDQVDVPEKVEELVHYGMHR
                     HFTEGCSMIFWGGGVKKGQVIGETAPERPFSAITEPIVIDQVHQSIYHALGISPDTHY
                     EVEGRPVYTTPDGQGKYIRELFS"
     gene            complement(3143..5608)
                     /locus_tag="PKM27_18830"
     CDS             complement(3143..5608)
                     /locus_tag="PKM27_18830"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNR09383.1"
                     /translation="MINRILFLANLLLIGTLSFLRLEEKPEDPELTRIIRDIKKQGKK
                     ADTALLGALNIKVRAIWTNRCFQCHSSEKRKGMLALDTYEAVLAGGEEGPVLVPGNAS
                     KSEMIRRLKLPRSNREAMPKKGQALSPEQVKAIETWIDFGAYWKDSAVQLFYEAPLSL
                     EKPIFPEYDGGHSHPVDRFTASYFKTYKIKWPKSLSDAEFLRKVHLDITGLLPSPDSL
                     AAFLANEDPDKRNHIINRLLADQENYALHWLSFWNDLLRNDYSGTGFITGGRKQISSW
                     LYSSLYEDSSYTRMVKSLVNPHPASEGFIAGIQWRGDVNSSQRVEMQAAQNISQSLLG
                     TNLKCASCHNSFVNNLSLAQSYGFASVFSKLPLEMYRCDVATGTIAEPAFIYPQLGPI
                     TADSLPDRLVQLAEVMTSAQNGRLYRTLVNRIWARLMGRGIVGKVDDMDQKPWSQELL
                     DYLAADFVEKGTGLKDLIRLITSSKIYQSRSIDYGSEEEIRNSDFIFRGPAKKRIQAE
                     QMADILSEVIQPVYSGVSFDPGAYHVPAAWIWYPEKEFDRIVLPRPDTVYFRKILNLP
                     AMARAELLVTADDEYELFINGKPVTSGKDYRYFQPVDPGEALIKGKNCIAVKAINKGY
                     LANPAGLLFHMRIHLPDKDTLQIYSDTSWLVSKTVSGESWKQVKYKDRDWVKIHRYGR
                     YSSSHWGMLRHFNFDAKEQPPFSRASLVSADPFLKALGRPTRENVTTARSQEAGLLQA
                     IALQNDPLLHQRVREGSANWVNKYQGKESDMIDRLYLHLLGRIPSAKEKTKLMEYYKG
                     HPGKESVADIIWALLMSPEFQMI"
BASE COUNT         1365 a         1417 c         1471 g         1583 t
ORIGIN      
        1 aaacgcaaag ctgtttttct tcataaagca gatacaaaat tttcagtctt acgttgttac
       61 ctgccaattc aagtccttgt gataagtagt caaaagttcc attgagttct gaaaccctgt
      121 ctttacagcg attgatttgt ttaatgtccg cctgttgtct aatgcaagaa atgttgtcca
      181 tacggcaaag ttagtcaatt tgtttattta agcaaatgct aaaatacaaa atgtcaaaat
      241 aaacccgatt ttgtctgtcg ggttgtgtcg tcttggcctt gccactaaca tttgtgttta
      301 cgcaatattt cacaaagcta cctgtatcat tcagtccata cgtatttcca ttttatctat
      361 cgggctgtgc atcctgaatt tgtttgcatc ggtaatatgt gtatatattt ctgtggtttt
      421 cagtgaatta tggcccaatg cagcttgaat ttgccttaga tctactcctg cttcaagcat
      481 atgggtggcg taggagtgcc ggagggtgtg cactgtagcg tatgcatcag ctcctgaacg
      541 ttgcaatgcc ctgtgaaaga cggcttgcac actccgggca ctgtaggggc ctccgtccgg
      601 gccttcaaca aaccatatcg aaccggatat atttccaggt atttttgcat taacagggcc
      661 atcctgtccg gcagtattac atatcggtct tttttccctt taccggcttt gatcagaatc
      721 ctcttctggc cgcggtcaat atcggccttg cgaagtttga tcaattcact gatcctgagt
      781 ccacaggcat agattagcgt caacatggtc cggtgcttga ggttctcaca agaattgagc
      841 agccggttca cctcatccaa actcagcgtc ccaggtaatt ttattggtct tctcggacgg
      901 acctcccaaa aggcacgagc ctcattcctg attttttcaa aataaaattt taaggcatta
      961 attatagtat tctgatacgc ttcagaccac ttgttttttt cgatttcgct ccggagccac
     1021 cgtttgaaat cttccagggt ccagtccttt ggatgcattg accggcactg gtacagaaag
     1081 tatttaaagg aactgcaata ggtacggata gtattgaggc tgtaacgctg tacatataac
     1141 tgagtataat aggtgtcgaa ttccgcctga agatccgaag gtaaattacg ctccgcggat
     1201 ttatccgcag gtgccctggt atccgttttg actatttcaa aggcggccaa agattcctgg
     1261 caccgtctcc acacctcttt ttcataagga atacaccagc ttttggtacc ggggttccaa
     1321 tgactccggg gtattttttt aatcaatgca ttgcaggccg gatcaaaatg ctcagtccgt
     1381 atacctatat gtttttggcc ccgggtttcg ataggaaata taaagatctt catagtggtt
     1441 atttttttat aagtatgaca aaggcccgcc tgaatcaccg ggtgttcaat cttccattta
     1501 gctaaaataa aacctaaacc tataattttc aagacattag cttatttctt ttgacggatg
     1561 gctatattaa ccatttgtag gcgggtatta atcgtttgtt gtcgaatgta gccggatgta
     1621 tccgtttgta ggcggaaata aaagatgatt ggtaaagtgt ccgccaggac cacagatcca
     1681 ctgtccaaac tggcaaactg cctaactgat aaactgacta actaaataac tctctaatat
     1741 acttcccctg accatccggc gtcgtataca ccggccttcc ctccacctcg taatgggtgt
     1801 ccggggagat gcccagggca tggtagatgg actgatggac ctggtcgatg acaatgggct
     1861 cggtgatggc gctgaaaggc ctttcgggag ccgtttcacc gatcacctga ccttttttca
     1921 caccgcctcc ccagaagatc atgctgcatc cttcggtaaa atgacggtgc attccgtaat
     1981 gcacgagctc ctccaccttt tccggaacgt ccacctggtc aagcactttc tggtccggac
     2041 gtccttcgat cagcatgtcg cggctgaatt cactggccag gatcaccagg gttcggtcca
     2101 gcagtcctct ctcatgcagt tcagtgatca attggtgaat gggttggtcc acctgttttt
     2161 tcatctccag ggcgcgggta tgcccgtttt catgggtgtc ccagccgagg aagggttcat
     2221 attccgtggt gacactgata aaccgcgctc cgcgttcgac cagtctccgg gccatcaggc
     2281 aacccaggcc aaagcggccg gtattatatt gatcgtatat ctctttgggc tcttcatgca
     2341 ggtcaaaggc tttggcttcc ggggatttta gcagggcata ggatctttcc atgctgcgca
     2401 tcagggattc ctgctgatat tcatgtgcat tggccagggg gccggccttg accagttccc
     2461 ggtagagttt ttgccttctt tcaaaatggg cgaggtccat gccggcgggc ggggaaatgc
     2521 tctccaggcc attcaccggg tcggggatgc tgaagggtcc gaattccgat cccaggaatc
     2581 cggcggaatg aaaagcttta agttcttcgc cttctcccac ggtaaacctc tgcccgatat
     2641 caataaatgc gggcatgact tcctgttttg ggcccagttc tttggcgatc caggcgccga
     2701 tatggggtgg ctggatgctc tgcggcgggg ggtaacaggt atgccaatgg tattggtgcc
     2761 gggtatgcag gataaaacca aggtccgcag cgcggtagga tcggatcagg gtacccaggt
     2821 gcatggtatc ggcgatcctt tccaggcctt cggaaaaacg gataccgtcc aggcgggtgg
     2881 gtatggaagg aaaggtactg aatacggatg cgctgtccat gccttttacg aaggggctgt
     2941 atttttttgg atcaaaagtc tccgtatggg ccattccacc agccatccaa aggaggatca
     3001 ccgaatctgc agtggatgga agggaatgcc tgcccgtttt gcatccggcg agaaagggaa
     3061 ggccggagac tccggtagac aggccggcca gggtggccag tctcattttc ccaataaatt
     3121 ctcttctcgt cagttccatg gttcagatca tttggaattc aggggacatc agcagggccc
     3181 agatgatatc cgccacgctt tccttcccgg gatgcccttt ataatattcc atcagttttg
     3241 ttttttcttt cgcggaggga attcttccaa ggaggtgcag gtagaggcgg tcaatcatat
     3301 ctgattcctt gccctggtat ttatttaccc agtttgcaga accttcccga accctttggt
     3361 gcaacaaggg atcgttttgc agtgcgatgg cctgtaacaa gccggcctcc tggctcctgg
     3421 ccgtggtcac gttctcccgg gttggacggc ccagggcttt caggaaaggg tctgcactga
     3481 ccagggaagc cctcgaaaac gggggctgct ccttagcatc gaaattaaaa tgacgcaaca
     3541 tcccccagtg gctgcttgaa taccttccat agcggtgaat tttcacccaa tcccggtcct
     3601 tgtatttgac ctgtttccag gattcgccgg aaactgtttt ggaaaccagc caggaggtat
     3661 cggaataaat ctgcaaagta tccttgtccg gcaaatgaat gcgcatgtga aaaagcaagc
     3721 cggctggatt ggccagataa cccttgttga tcgccttcac ggctatacaa ttttttcctt
     3781 tgatcaaagc ctcgccggga tcgacgggct gaaaatagcg atagtctttc cccgaggtga
     3841 ccggtttgcc attaataaac aattcatatt catcatccgc agtgaccagg agttctgccc
     3901 tcgccatggc tggcaggttc aatattttcc ggaaatacac cgtgtccggc cggggcagca
     3961 cgatccggtc aaattctttt tcagggtacc agatccaggc tgccggaaca tggtaagccc
     4021 cggggtcgaa tgaaaccccg ctgtagacgg gttgtatgac ttcgctgagg atatcggcca
     4081 tctgttctgc ctgaattcgt ttttttgcag gtcccctgaa tataaaatca gaattcctga
     4141 tttcctcctc gcttccataa tcgatggaac gggattgata aatttttgaa ctggtgatca
     4201 gccggatcag gtctttcagg ccggttccct tttccacgaa gtccgcagcc aggtaatcca
     4261 gcagctcctg gctccagggt ttctggtcca tatcgtctac cttgccgacg atgccccggc
     4321 ccataagccg ggcccagatc cggttgacca gggtccggta cagccgcccg ttttgggctg
     4381 aggtcatcac ctcagccagt tgtacaagtc ggtccggcag actatcggcg gtaatcgggc
     4441 caagctgagg atagataaaa gccggttcgg cgatcgtccc ggtggcgaca tcgcaacggt
     4501 acatttccag cggcaatttg gaaaaaacgc tggcgaaacc ataggattgg gccaggctca
     4561 ggttgtttac aaaactgttg tgacaggaag cgcatttcag attggtgccc agcaggctct
     4621 gggatatatt ttgggcagcc tgcatttcga cgcgctggct cgaattgaca tcccctctcc
     4681 attgaatgcc tgcaatgaac ccttccgaag ccgggtgggg attgaccagg cttttaacca
     4741 tgcgggtata ggaactgtct tcataaagcg agctgtaaag ccaggacgat atttgttttc
     4801 tccccccggt gataaagccg gttcctgaat aatcattccg gagcaggtca ttccagaaac
     4861 tgagccaatg cagggcatag ttttcctggt ccgccagcag tctgtttatg atatgatttc
     4921 ttttatccgg gtcctcattg gccagaaagg cggccaggct gtccggtgag ggcaagagtc
     4981 cggtgatatc caggtggact tttcgaagga attcggcatc cgaaagggat ttgggccatt
     5041 ttattttata cgttttgaag taggacgcgg taaaacgatc gactggatgg ctatgccccc
     5101 cgtcgtattc gggaaatatg ggtttttcca ggcttagggg agcctcatag aataattgca
     5161 ccgcgctgtc tttccagtaa gcgccgaagt caatccaggt ttcaatagcc ttgacctgtt
     5221 ccgggctgag cgcctggcct tttttaggca tggcctcccg gttgctcctt ggcaatttga
     5281 gcctcctgat catttcgctt tttgaagcat ttccgggtac caggactggc ccttcttcgc
     5341 ctcctgcaag gaccgcttca taggtatcca gtgcaagcat gccttttctt ttttctgaac
     5401 tgtggcattg gaaacaccgg ttggtccaga tggccctgac ctttatattg agggcaccga
     5461 gcagggcggt atctgccttt ttaccctgct ttttaatatc ccggatgatc cgggtcagtt
     5521 cgggatcttc cggcttttct tccagacgca ggaaggagag ggtcccgatc agaagcaggt
     5581 ttgcaagaaa aaggatccgg ttaatcattg aattcaaaag gcggtcaaat tagaaaaaaa
     5641 agataaacaa tttatgaagg aggaagggag gaaggcgaaa ggcgaaaggt caaaggcgaa
     5701 agttcaaagg cgaaaggcga aaggcgaaag gcgaaaggag cacaagtaca cattgatcat
     5761 ggctgaaagc cgatagctca aagctgtgca tgttgcacaa ctcataaaaa acacattaaa
     5821 ttattcggtt atgtga
//
LOCUS       DAOQXT010000183         5779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_14192_length_5779_cov_5.058351, whole genome shotgun sequence.
ACCESSION   DAOQXT010000183 DAOQXT010000000
VERSION     DAOQXT010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14192_length_5779_cov_5.058351"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1309
                     /locus_tag="PKM27_18835"
     CDS             <1..>1309
                     /locus_tag="PKM27_18835"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNR09384.1"
                     /translation="IFNDLNADRYNILVTDASGCVASLDTLLVQPPLFDTLSVIVTPE
                     SCFPGRDGRIEINMTGGTAPYQFIWDSAFANTNIVTGLSSGNYRVTIRDANNCISNFD
                     TSNVLSFPFILPTVPVSIDTTPILCNGQGATLTARPGPGYTISAYAWSDGQSTAVAQN
                     IKADTLTWVEVTDDKGCKGRDSVVLSQPPALAWIDSITQLTDPPCPDIDQGRILVEAT
                     GGTGPYNYTLSNGTSNNFGVFDRLLAGRYTVTVVDANNCPGLVLDTLLTDPPRIVAVF
                     DSAAFRNTLCATPGDCSGQARLLLSGGTDPLNNFLVQWESGEQSLGNVVSLADSLCSG
                     QQLVRIFDGNGCRTQLNFTINAPEAISRDAVRTMTRDITCFGSNDGNINYLAFGGTSP
                     FRYLWSDNDTNSVRTGLAAGRYRVTVTDLNNCMFTDSLTLSQPP"
     assembly_gap    1310..1409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1410..1949
                     /locus_tag="PKM27_18840"
     CDS             <1410..1949
                     /locus_tag="PKM27_18840"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNR09385.1"
                     /translation="FNIELGDDTRIRLGDSLRVTVISNNPVNRVQWVNIDGLTCVTGD
                     CSVVLLSPKKKTTFSATAFDGNDCAAFDNITLDVDLKRAVFIPNIFRPDQLSANQEFK
                     LFANAGIRVIHSGKIFNRWGDLVSDVQNLGPDPGGVVIWDGRFRSTEAQPGVYVYVID
                     VEFTDGERLIYKGDVTLLR"
     gene            2223..3446
                     /locus_tag="PKM27_18845"
     CDS             2223..3446
                     /locus_tag="PKM27_18845"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HNR09386.1"
                     /translation="MNRPYLFCLVMLGLAYLLPGQDLDLLIKNGHVLDPKNNIDGIMD
                     VGVKGGKITEVARSISKTAKKTIDATGLYVTPGLIDIHGHHFFGTKSNAYLSNSYTAL
                     PPDGFTLRTGVTAVVDAGGSGWRNYEDFKNQTIGHSKTKVYAFLNIVGAGMSGGAYEQ
                     NLADMDAKLTAMTARSNKDRIVGIKLAHYGSRDWTPALRAAEAGRLADIPVMIDFGGC
                     DPPLSLDTLLNHVLRPGDIFTHCYAQVAGRESVVKDGKVMPWALKAQQRGIIMDVGHG
                     GGSFVYAQAIPAIQQGLKPNTISTDLHTGSMNAGMKDMAMTMSKLINIGMSMKEIVEA
                     STWKPAQVIKKTELGHLSAGAAADIALLKIEKGNFGYYDVRQKKMPGDKRIVCEMTIL
                     DGEVVWDLNALSFTE"
     gene            3462..5192
                     /locus_tag="PKM27_18850"
     CDS             3462..5192
                     /locus_tag="PKM27_18850"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNR09387.1"
                     /translation="MIPLNGQGTAVSLCKTCLLLACVFLFSRCKKPQDLFVPDGFEVT
                     VVADSIPGGARHIAVSENGDLYVKLLQTKKGGNAALRDTDGDGKMDSIATFGGYPEVG
                     RYGTSMRIYQGYLYFSTELVVYRMKLSPGSLVPEGEPEVILTDDHPHRSHEHIAKPVT
                     FDDQGHIYIPFGAPSNACQEFNRTPFSVGMDPCGMLEDHGGVWRFDAHKTNQTQKDGY
                     KYATGLRSLVGIEWNPLDKKLYAVMHGRDDLLRLWPNLFSPWQSAVLPAEEFLRIEEG
                     SHAGWPYCYYDQMQGKKVLAPEYGGDGKIVGRCDSFYNPIVGFPGHWAPNDLFFYNGD
                     QYPEHYKNGAFIAFHGSTNRAPYPQAGYFIGFMPYRDGEFKGPVEVFADGFARLDTIV
                     NVSDAVYRPMGIAMGPDGSLYFAETEKGRVWKVSYKGNKEKFGPAQLASMAQRALTAN
                     HIRTPDEVLDNLDRGKKADGEKAYRVYCGACHQNNGLGASGRFPPLASDWVSGDKNRL
                     INVVLQGLEGDILVNNEIWNGTMPRHDFLKDEDIATILTYIRSSFGNQADAVTPEEVK
                     AQRDQLLEKK"
     gene            5349..>5779
                     /locus_tag="PKM27_18855"
     CDS             5349..>5779
                     /locus_tag="PKM27_18855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNR09388.1"
                     /translation="MNTNRRSLFKKLSLGLAGFLGIKTVSAAEESTSTATPHNEITMD
                     QNIPLFSGARTFGKLLFIAGKGAHFEGDIRAHTKHVLDEVQKELEKNGSSMNKVLKVN
                     VYLNDLKDYQAMNETYRGRFGDNPPVRTTIAAAGGIPGNSLV"
BASE COUNT         1407 a         1513 c         1460 g         1299 t
ORIGIN      
        1 ccatttttaa tgacttaaat gctgaccggt acaatattct tgtgaccgat gcctccggct
       61 gtgttgcaag cctggatacc ttattggtac agccgcccct cttcgatacc ctatctgtga
      121 tcgtcactcc cgaatcctgt tttccgggtc gggatggcag gatcgaaatc aatatgaccg
      181 gggggacagc accctaccaa tttatctggg attctgcctt cgcaaatacc aatatcgtta
      241 ccggtctgag cagcggcaat taccgggtta ccatcaggga tgccaacaat tgtatttcca
      301 atttcgatac ttccaatgtg ttgtccttcc ctttcattct tcctacggta cccgtaagta
      361 ttgacaccac ccccatcctc tgcaacggac aaggggcgac cctcaccgcc aggcctggac
      421 cgggatatac gatctccgcc tatgcctgga gcgatggaca atccactgcc gtagcccaaa
      481 acatcaaagc agataccctg acctgggtag aagtcacgga tgataaggga tgcaagggca
      541 gggacagcgt cgtattgtcc cagccacccg ccctggcatg gatcgactcg atcacccaac
      601 tgaccgaccc gccctgtccc gatattgacc aagggaggat tttggtggaa gccacgggag
      661 gtacaggtcc atacaactac accctgtcca atgggaccag caataatttt ggagtctttg
      721 accggctttt ggctggcaga tacacggtca ccgtggtgga tgccaataat tgtccgggcc
      781 tggtactcga caccttactg acggacccgc cccggatagt cgccgttttc gacagcgcgg
      841 ccttcagaaa taccctctgc gccacccccg gagattgcag cgggcaggcc cgcttattgc
      901 tttcgggagg gactgatcca ttgaataatt ttctggtgca atgggagtcc ggggaacaaa
      961 gcctgggtaa tgtggtctcc cttgccgaca gtctgtgctc cggccagcaa ctggtacgca
     1021 tattcgacgg caacggctgc cgcacccaac tgaactttac catcaatgcc ccggaggcca
     1081 tatcccggga tgccgtccgg acgatgaccc gggacatcac ctgttttggt tccaacgatg
     1141 gaaacatcaa ttacctggct ttcggtggca cctcgccatt ccgttacctc tggagcgaca
     1201 acgataccaa ttctgttcgt accgggctcg ccgcgggcag gtatagggtg acggtgaccg
     1261 acctcaataa ttgtatgttc accgattccc tgaccctcag ccaacccccn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tcaatatcga attgggcgat gatacccgga
     1441 tccggctcgg cgacagtctc cgggtcaccg tgatcagcaa caatcccgtc aaccgtgtcc
     1501 aatgggtcaa tatcgatggc ctcacctgcg tgaccgggga ttgttcggtg gtgctccttt
     1561 ccccaaagaa aaaaactact ttttcggcca cggcttttga cggcaacgat tgtgcggctt
     1621 tcgacaatat caccctcgat gtggacctta agcgggccgt atttatacct aacattttcc
     1681 gtccggacca gctcagcgca aaccaggaat tcaaactgtt tgccaatgcc ggcatccgag
     1741 tcatccactc cggaaaaatt ttcaaccgct ggggtgacct ggtttccgat gtacaaaatc
     1801 tcgggccgga tccgggtgga gtggtcattt gggacggccg tttccggagt accgaagccc
     1861 agcccggcgt gtacgtctat gtgatcgacg tggaatttac ggatggggaa aggctgattt
     1921 acaagggcga tgtgactttg ttgagatagg gtagcagcca tcagcaatca gcaatcagcc
     1981 atcagcagtc agccttcagc agtcagcctt cagcagtcag ccttcagcag tcagccttca
     2041 gcagtcagcc ttcagccatc agccatcagc agtcagccac ctatgctgaa agctgaaaaa
     2101 ctgaaagctg aaagctgaat actgaatgct cttttttact tattttactc ccggtaaggg
     2161 taatgaggtt ttattgcctt tcaagtccaa ttattgactc taacttcgta agtaaaaaat
     2221 gtatgaatag accttatttg ttttgcctgg tcatgctggg cctggcttat ctccttcccg
     2281 gacaggacct ggaccttttg ataaaaaacg gccatgtctt ggatccaaaa aacaatattg
     2341 acgggattat ggatgtcggg gtgaagggcg gcaagatcac tgaagtggcc cggtcgatct
     2401 ccaaaacagc caaaaaaacc atcgatgcga ccggcctcta tgtaacgccg gggctcatcg
     2461 atattcacgg ccaccatttt ttcgggacta aatcgaatgc ctacctgagc aacagctata
     2521 cggccctgcc cccggatgga tttaccctcc ggactggggt caccgcggta gtggatgccg
     2581 gaggctcggg ttggcgcaat tatgaagatt tcaaaaacca gaccataggg cattccaaaa
     2641 caaaggtgta cgcgttttta aacatcgtcg gtgcggggat gagcggtggc gcctacgaac
     2701 aaaacctggc ggacatggat gccaagctga ccgccatgac ggcccggtcc aataaagacc
     2761 ggatcgtagg tatcaaactc gctcattacg gatcccggga ctggacccct gcactcaggg
     2821 ctgcggaagc agggcgcctg gcggatattc cggtcatgat cgattttgga ggttgtgatc
     2881 cacctttgtc cctggacacc ttattaaatc atgtgcttcg tcccggggat atatttaccc
     2941 attgttacgc ccaggtggcg ggccgcgagt ccgtggttaa agatggaaag gttatgccct
     3001 gggctttgaa agcccagcaa aggggaatca tcatggatgt gggccacgga gggggcagtt
     3061 ttgtctatgc gcaggccatc ccggcgattc agcaggggct caaacccaat accatcagta
     3121 cggacctcca tacgggaagc atgaatgcgg gcatgaaaga catggccatg actatgtcca
     3181 aactgatcaa tattggtatg agcatgaaag aaatcgtgga ggcgagtacc tggaagccgg
     3241 cacaagtgat caaaaagacc gaattgggac acctcagtgc cggagcggcg gccgatatcg
     3301 cattgctgaa aatcgaaaaa ggcaatttcg gttattacga tgtgcggcag aaaaaaatgc
     3361 cgggggacaa aaggatcgtg tgtgaaatga ctattctcga cggtgaagtg gtctgggacc
     3421 tgaacgccct ttcctttact gaatgatttt cctaggttaa catgatacca cttaatggcc
     3481 agggaactgc cgtttccctt tgcaaaacat gcctgttgct tgcatgtgtc ttcctgtttt
     3541 cccgctgcaa aaagccgcag gacctcttcg tccccgatgg atttgaagtg accgtggtgg
     3601 ccgacagcat tcccggcggc gcgaggcata tcgcggtgag tgaaaacggg gacctatatg
     3661 tcaaactgct gcagaccaaa aaagggggta atgcagcgct ccgagatacc gacggcgatg
     3721 gaaagatgga cagcatcgcc acctttggtg gttatccgga ggtcggccgt tatgggacct
     3781 ctatgcgtat ttaccaggga tatctttatt tcagcacgga attggtcgta taccgcatga
     3841 aactcagtcc cggcagcctg gtgcccgaag gagaaccgga agtcatcctg accgatgacc
     3901 acccccacag aagccatgag catattgcca aacccgtgac ctttgacgac cagggccata
     3961 tctatatacc ctttggcgcc ccttccaatg cctgccagga attcaaccgg accccgtttt
     4021 ccgtgggcat ggacccctgc ggcatgctcg aagaccatgg gggggtgtgg cgttttgatg
     4081 cccacaagac caatcagacc cagaaggatg ggtataaata tgccaccgga ctgcgcagcc
     4141 tcgtaggcat tgaatggaat cctttggata aaaagttgta cgcggtgatg catggccgtg
     4201 acgatttgtt gcgtctctgg cccaatcttt tcagcccctg gcaaagcgcg gtgcttccgg
     4261 cggaagaatt tttacggatt gaagaaggca gccatgccgg atggccctat tgttattacg
     4321 accagatgca gggcaaaaaa gtgcttgccc ccgaatacgg cggggatgga aagatcgttg
     4381 gccgctgcga ttctttttat aacccgatcg tcggttttcc gggccactgg gcgcccaacg
     4441 acctgttttt ttataacggg gatcaatacc ctgaacatta taaaaacggc gccttcatcg
     4501 cctttcacgg ctccaccaac cgggcgcctt atccccaggc cggatatttt attggcttca
     4561 tgccctatcg cgatggggag tttaaaggac cggtggaagt ctttgccgac ggttttgcca
     4621 ggctggacac cattgtcaat gtgagcgatg cggtctaccg cccgatgggc atcgccatgg
     4681 gccccgacgg atcgctttac tttgcggaaa cggaaaaagg ccgggtctgg aaggtgagtt
     4741 ataaagggaa taaggaaaaa ttcggccctg cccaattggc gtccatggcc cagagggcgc
     4801 tcaccgccaa tcatatccgg acgccggatg aggtgctgga caacctggac cgcgggaaaa
     4861 aagccgacgg agaaaaggct tatcgtgtgt attgcggagc ctgccatcaa aataacggcc
     4921 tcggggccag cggaagattc cctccccttg cttccgattg ggtgtccggg gataaaaacc
     4981 ggctcatcaa cgtcgtcctt cagggattgg aaggagatat cctggtcaac aacgaaattt
     5041 ggaatggcac tatgcccagg catgactttc tgaaagacga agacattgcc accattctga
     5101 cttatatccg cagcagtttc ggcaaccagg ccgatgccgt aaccccggaa gaagtaaaag
     5161 cccagcgtga tcaactcctt gaaaaaaaat gattatttta ctaacttctt atgttaaaat
     5221 gattccttaa ctctattaaa tcccattaat cctacaaatc ctatcaatcc taataatcct
     5281 aataatccta ataatcctaa taatcctacc aatcctaata atcctataaa tcctataaat
     5341 cctataatat gaatacaaac agaagatcat tgttcaaaaa attgtccctc ggcctggccg
     5401 gtttcctggg tatcaaaacc gtaagcgccg cggaagaaag cacctcgacc gcaactccgc
     5461 acaacgagat caccatggac cagaacattc ccctcttttc aggggcaagg accttcggca
     5521 agctgttgtt cattgccggc aaaggcgcgc attttgaagg ggatatacga gcccatacca
     5581 aacatgtatt ggatgaagtg cagaaggaac tggaaaagaa cggttcctcg atgaataaag
     5641 tgctcaaggt gaacgtctat ctcaatgacc tgaaagatta ccaggccatg aatgagacct
     5701 atcgtggccg ctttggggac aatccccccg tccgcaccac catcgccgcg gcgggaggca
     5761 taccgggcaa ttccctggt
//
LOCUS       DAOQXT010000184         5727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_14337_length_5727_cov_5.154443, whole genome shotgun sequence.
ACCESSION   DAOQXT010000184 DAOQXT010000000
VERSION     DAOQXT010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14337_length_5727_cov_5.154443"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKM27_18860"
     CDS             complement(<1..228)
                     /locus_tag="PKM27_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09389.1"
                     /translation="MIKRLLLSLFCLSGTAFVFGQNGYYFPGATGFNTSIPNPEQFLG
                     YPIGSHHTRHDKVVEYIRELDRLSDRMTTRII"
     gene            complement(367..>2111)
                     /locus_tag="PKM27_18865"
     CDS             complement(367..>2111)
                     /locus_tag="PKM27_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNR09390.1"
                     /translation="QYADLTDLKGGEQVMDFEEIKNGPGIPNEENEALELEISDNPQE
                     VVAGNIQNLAEEDQKAQQELRYASLEPYDPDAELSKFKLPSVELLQDYTDQNFEIDRA
                     ELEANKDQIIETLMHYKIEITKIRATVGPTVTLYEIIPAPGVRISKIKNLEDDIALNL
                     SALGIRIIAPIPGKGTIGIEVPNKKKQIVSLKEVLVSEKFQSSKMDLPIALGRTIANE
                     VFVADLAKMPHLLIAGATGQGKSVGINAILMSLLYRKHPSQVKLVLIDPKKVELFPYA
                     KLENHYLAYLPDQDEPIVTETNKVIYTLNSLCIEMDTRYDLLKKAEARNIKEYNKKFT
                     ARQLNPEKGHRYLPFIILVIDEFADMIMTAGKEVELPIGRLAQLARAVGIHLIIATQR
                     PSVNIITGVIKANFPARLAFKVSSKVDSRTILDTGGADQLIGSGDMLLSVGGDMIRLQ
                     CAFVDTPEVENVLHFIHDQRGFPQPYFLPEYTGEEGASGTEKAVLEDLDDMLDEAARL
                     VVQNQHGSTSMIQRRLKLGYNRAGRIMDQLELLGVVGAGEGSKPREVLVSSEVELNRI
                     LDRIRGGKERDTLF"
     assembly_gap    2112..2211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2212..3056)
                     /locus_tag="PKM27_18870"
     CDS             complement(<2212..3056)
                     /locus_tag="PKM27_18870"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNR09391.1"
                     /translation="MAKKKNSKPFWKRINPRDERWIKLAGIFTLFFSILLFVSICSYL
                     FTWKYDQDKVLNFSWKIFLQKDLAMANTLGRFGAFLSHQAIYWSFGLPAFAFVYIGLL
                     YAWKWIRNGRYRLMGRTLGILWVMLYTSVLLESFLGRLAFPFGGAIGEWIAYYLQNFL
                     GLAGLIGLLLLVVILLAVWNFNPDFSKLRLPSYQFSGEGAFSGGTASFLNYFRKPNKD
                     PLPEDRERESILSETGVERIIERDFKDPEENDLHESPTLELEVYKPQYADLTDLKGGE
                     QVMDF"
     gene            complement(3344..4849)
                     /locus_tag="PKM27_18875"
     CDS             complement(3344..4849)
                     /locus_tag="PKM27_18875"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HNR09392.1"
                     /translation="MITGNIVGFEVLGDGQTLQGVNPASQELLEGRFSIATQNEIDLA
                     LDKAERAFPIFRKWSPEKRAGFLDLIAKNIIDLGDVLVKRAMEETGLAEARILSERGR
                     TTGQLNMFAHAIRKGQWAEPSIDHAIPDRQPLPKADIRKRLVPLGPVVVFTASNFPLA
                     YSTAGGDAASALAAGCPVIVKAHESHPGTNALVAEAILMAAKEMEAPDGVFSTLYGYG
                     PELGQALVKDPRVKAVGFTGSYGAGRAIMDSAAARPEPIPVYAEMGSLNPVLVTRSAL
                     EKRAVSIGEQLGASMTLGAGQFCTKPGLFLVQDHPQFESFIQGLEQKILAATATPMLN
                     ARIHSNFLKSISAFSSELSLLNLNKAEGPLVSPAIAMVSGQEFIDHPKWHHEVFGPFA
                     IVIKCNGPEQMITILEMLGGQLTATIWSESGDPDNSSLLEAMEQTAGRLVWNGVPTGV
                     EVCESMMHGGPFPATSHPHFGAVGVSALKRWTRPVAYQNFPDEYLPEILKG"
     gene            complement(4846..>5727)
                     /locus_tag="PKM27_18880"
     CDS             complement(4846..>5727)
                     /locus_tag="PKM27_18880"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNR09393.1"
                     /translation="ALTTKMKEDASLDLPAFQKHLDSQIREGIHGAIIGGSLGEASTL
                     RPEEIIQLLVAAKETASGRIPVLVNIAESSTDNAVNAARAAEAHGADGLMLLPPMRYK
                     ADDDETVVYFKTVAQNTSLPIMIYNNPYDYKIAVTIDMFEQLAAYSNIEAVKESSRDI
                     TNITRMINAFGSRFKILGGVDTLALECLASGADGWVAGLVNAFPAETVAIYQWVKTGR
                     LQEALTVYRWFMPLLELDIHAKLVQYIKLAEAATGLGTEHVRPPRLPLSGLERERILT
                     IIHDAIQSRPQIKKHKP"
BASE COUNT         1292 a         1408 c         1515 g         1412 t
ORIGIN      
        1 gatgatccgg gtagtcatcc ggtcactaag acggtccagt tccctgatgt attccactac
       61 cttatcgtgc cgggtatgat ggctgccaat gggataaccc aggaattgtt ccgggttggg
      121 gatggaagta ttgaatcccg tcgccccggg gaaataatat ccgttttggc cgaaaacaaa
      181 agctgtcccg gaaaggcaaa acagggataa aaggagtctt tttatcattg gtaagaatat
      241 aagattctca agataagggg ttggatggaa atgccaaatc gaaataaaat cagcacttta
      301 tcgtgaggaa aagtgcatat ccccaacttg ctaacttacg aacttgctaa cttgttaact
      361 tgctaactaa aacaaagtat ccctctcctt accaccccgg atccggtcca ggatcctgtt
      421 gagttccact tcgctgctga ccaggacttc tcgcggtttt gaaccttctc ccgcaccgac
      481 cacacccagc agttcaagct ggtccataat gcggccggcc cggttgtagc cgagtttgag
      541 cctgcgctgg atcatggagg tgctgccgtg ctggttttgc accaccaacc gggcggcttc
      601 gtccagcata tcgtccaagt cttccagcac cgctttctcg gtgcctgaag cgccctcctc
      661 gccggtatat tcaggaagaa aatagggctg ggggaagccc ctctgatcgt gaatgaaatg
      721 caatacattt tccacttccg gagtatccac gaaagcgcac tgaaggcgga tcatatcgcc
      781 cccgacgctc agaagcatgt ccccgcttcc gatcaactgg tcggcgcccc cggtatccag
      841 gatggtgcgg gagtccactt tggaagatac tttgaaagcc aggcgggccg ggaaattggc
      901 cttgatcacc ccggtgatga tgtttacgga aggcctctgg gtggcaatga tgaggtgtat
      961 gcctacagcc ctggccaact gggccagtcg accgatgggc aattccactt cttttcccgc
     1021 ggtcatgatc atatcggcaa attcgtcgat gacgaggatg atgaaaggca ggtaccggtg
     1081 acccttttcc gggttgagtt gacgggccgt gaatttttta ttgtattcct tgatgttcct
     1141 cgcttccgct tttttgagca ggtcataccg ggtatccatc tcgatgcaga gcgaattcag
     1201 ggtataaatc accttgttgg tctccgtcac gatgggttca tcctgatcgg gcaggtaagc
     1261 caggtaatga ttttccagtt tggcatatgg aaacaattcc acttttttgg gatcgatcag
     1321 gaccagcttc acctgggagg gatgtttgcg gtacaggagg gacatgagga tggcattgat
     1381 gcctacggat ttgccctggc cggtggcccc ggcaataagg aggtggggca ttttagccag
     1441 gtcggccaca aagacttcat tggcaatggt acgtccaagg gcgatgggca gatccatttt
     1501 ggaggactgg aatttttcag agaccagcac ttccttgagg gagacgatct gttttttctt
     1561 gttaggcacc tcgatcccga tcgtgccttt tccgggaata ggcgcaatga tgcgaatgcc
     1621 cagggcggaa aggttcaggg cgatatcgtc ttccaggttt ttgattttgg aaatccgtac
     1681 gccaggggcg gggatgatct cgtacagcgt cacggtaggt cctacggtgg ccctgatctt
     1741 ggtgatttca atcttatagt gcatcagggt ctcgatgatc tgatctttgt tggcttcgag
     1801 ttcagcccgg tctatttcaa aattctgatc ggtataatcc tggaggagtt cgacggaggg
     1861 aagtttaaac ttggacaatt cggcatcggg atcatagggt tccaggctgg cgtaccggag
     1921 ttcctgctgg gccttctggt cttcctcggc caggttttgg atattgcctg ccaccacctc
     1981 ctgaggattg tcactgatct ccagttcaag ggcttcgttt tcttcattgg ggatacccgg
     2041 cccgttttta atttcctcaa agtccatcac ctgttctccc cccttcagat cggtgagatc
     2101 ggcatattgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcaaagtcc
     2221 atcacctgtt ctcccccctt cagatcggtg agatcggcat attggggctt atatacttcc
     2281 agttccagtg tcggcgattc atgcaaatca ttttcctccg gatctttaaa atccctttct
     2341 atgatccttt caacacccgt ttcggagagt atggattccc tctcccggtc ctccggcaaa
     2401 gggtctttgt ttggtttcct gaaataattt aaaaaggaag cagtgccccc ggagaaagcg
     2461 ccttcgccgg aaaattgata cgagggtaaa cgaagctttg aaaaatcggg attaaaattc
     2521 cagactgcaa ggagtatgac caccagtaaa aggaggccga tgagcccggc caggcccagg
     2581 aagttctgaa ggtaataggc gatccattcg ccgatcgccc caccaaacgg aaaagccagg
     2641 cggcccagaa aactttccag cagcaccgag gtatagagca tcacccagag gatacccagg
     2701 gtcctgccca tgaggcggta acggccattg cggatccatt tccaggcata gagcagacca
     2761 atatagacga aagcgaaagc aggcaggccg aaactccagt aaatggcctg atgtgaaagg
     2821 aaggccccga accggcccag ggtattggcc atagccaggt ctttctgaag gaatattttc
     2881 caggagaagt tcaatacctt gtcctggtcg tatttccagg tgaagagata ggaacaaatg
     2941 ctgacgaaaa ggagaatgga aaaaaacagg gtaaaaatgc cagcgagttt gatccacctt
     3001 tcatccctgg gattgatcct tttccaaaaa ggtttactgt tttttttctt agccatgcgg
     3061 attcaataat tgtcggtacc gcggtaaaaa taggaagttt tattaaatat taaaataaaa
     3121 tgtaatttat attacactga ctttcagtta tgaatattct tatctatagg aatttgagaa
     3181 acagggattc atgtatagga attttaatga tcaacggagg ggccggccct ccggagtcag
     3241 ggatcaggtg gatatcacca ggcttaggac ctctggaaaa acatttcgtg aagggtaaag
     3301 cacaacacgg caatgataag aacccggaac cgggccttcg aatctatcct tttaaaattt
     3361 ccggtaaata ctcgtccgga aaattctgat aggccaccgg gcgggtccag cgttttaatg
     3421 cactgacccc caccgcccca aaatgaggat gactggtggc ggggaagggc ccgccatgca
     3481 tcatgctctc gcatacttct accccggttg gtactccgtt ccacaccagg cggccggccg
     3541 tttgttccat ggcttcgagc agggatgaat tgtctggatc gccggattcg ctccagatcg
     3601 tggcggtcag ttgtcccccc agcatttcca ggatggtgat catctgttcc ggaccgttgc
     3661 atttgatcac tatcgcgaaa ggaccgaaaa cttcatgatg ccacttggga tggtctataa
     3721 actcctgtcc actcaccata gcgatggcag gactgaccaa ggggccttct gctttattca
     3781 ggtttaacag ggaaagctcg gaggaaaaag cggaaatcga ttttaaaaaa ttgctgtgta
     3841 tccgtgcatt gagcattggt gtggcggtgg cggcaaggat tttttgttcc agaccctgga
     3901 taaaagattc aaattgggga tggtcctgta caaggaagag gccaggtttg gtgcaaaact
     3961 ggccggcacc cagggtcatt gaggctccaa gttgttctcc gatagagacc gcgcgtttct
     4021 ccagggccga ccgggtgacc aggacagggt tgaggctgcc catctccgca tagacaggga
     4081 taggctcagg cctggcggcc gccgaatcca tgatggcccg tccggcacca taagaaccgg
     4141 taaaacccac tgccttgacc ctgggatcct tgaccagggc ctggccgagt tcaggcccgt
     4201 aaccgtacag ggtagaaaat accccatcgg gcgcctccat ttcttttgcc gccataagga
     4261 tggcttccgc taccagggca ttggtgcccg gatgggattc gtgggcttta acgataaccg
     4321 ggcaaccagc agccagggcg ctggctgcat cccctcctgc agtggaatag gccaggggaa
     4381 aattgcttgc cgtgaagacc acgaccggtc caaggggcac cagtcttttt cggatatccg
     4441 ccttgggtaa gggttgccgg tcaggaatgg cgtggtcaat gctgggttcg gcccactggc
     4501 ctttgcggat ggcatgggca aacatattca actgtcccgt agtgcgtcca cgttctgaaa
     4561 ggatccgtgc ctccgccagt cccgtttcct ccatcgccct ttttaccagg acatcaccca
     4621 ggtcaatgat gtttttagcg atgagatcca aaaaaccggc tcttttttca ggactccatt
     4681 tcctgaatat gggaaaagcc cgttctgctt tgtccagggc cagatcgatc tcgttttgtg
     4741 tcgctatgga aaacctgcct tccagcaatt cctggctggc gggattgact ccctgaaggg
     4801 tttgtccgtc acccaacact tcaaagccca ctatattgcc tgtgatcatg gtttatgttt
     4861 ttttatttga ggtctggatt ggatggcgtc atggatgatg gtcaggatcc tttcccgttc
     4921 aagccctgaa aggggcaggc ggggtgggcg gacgtgttcg gttccaaggc cggtcgccgc
     4981 ttcagccagt ttgatgtatt gcaccagttt agcatggatg tccagctcca ggaggggcat
     5041 aaaccagcgg tagacggtca gggcttcctg gagccggccc gtttttaccc attgataaat
     5101 ggccacggtt tccgccggga aggcattcac cagtcccgca acccagccat ccgctcccga
     5161 agccaggcac tccagggcaa gggtatcgac ccctccgagg attttgaaac ggctgccaaa
     5221 ggcattgatc atgcgggtga tgttggtgat atccctgctg ctttctttca ccgcctcaat
     5281 attggagtaa gcggccagtt gttcaaacat atcgatggtc accgcgattt tataatcata
     5341 gggattgttg tagatcatga taggcaggga ggtgttttgc gccacggttt taaaatacac
     5401 cacggtttca tcatcatccg ctttatacct catgggagga aggagcatga ggccgtcggc
     5461 cccatgggcc tcggctgctc tggccgcgtt gaccgcatta tcggtggagg attcagcgat
     5521 attgacgagg accggtattc gtccggaggc cgtttctttg gccgccacaa gaagttggat
     5581 gatttcttcc ggcctgaggg tactggcttc cccaaggctt cctccgatga tcgccccatg
     5641 tattccttcc cggatctggg agtcgagatg tttttgaaag gcaggaaggt ccagggaagc
     5701 gtcttccttc atttttgtgg tcagggc
//
LOCUS       DAOQXT010000185         5601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_14744_length_5601_cov_5.614497, whole genome shotgun sequence.
ACCESSION   DAOQXT010000185 DAOQXT010000000
VERSION     DAOQXT010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14744_length_5601_cov_5.614497"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1347)
                     /gene="porV"
                     /locus_tag="PKM27_18885"
     CDS             complement(145..1347)
                     /gene="porV"
                     /locus_tag="PKM27_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNR09394.1"
                     /translation="MKKFYGAVLFSLSWFALADLSAQCRYDPATGKMVDALTGRECIN
                     TIVTAVPFLRITPDSRAGGMGDAGIAVESDAGSIYNNASLLAFAEKPRSISTTYSPWL
                     RALGLTDVYMAYVAGYTKIGDLQAIGGSLRYFSLGNIDYTDDNGAPLGSGKPNEFEVT
                     GTYSRKLSDQLAAAVSLKFIYSFLASDLNIGGNDIKPGIAGATDISFSYKKPVGQNFF
                     TAGLALTNLGTKISYTKANQEYIPANLGIGAAYTWNFDDYNKFTLTADINKLLVPSPY
                     ASTHPDYDKNGDQIPDYKQYNVVSSWFKSFGDAPNGFKEELQELMFSFGTEYWYNNQF
                     ALRAGYFRENPYKGNRQYLTLGLGVKYNVFGMNLSYLVPTARAATNRDPLANTLRFSL
                     IFDFEEQE"
     gene            complement(1428..4862)
                     /gene="porU"
                     /locus_tag="PKM27_18890"
     CDS             complement(1428..4862)
                     /gene="porU"
                     /locus_tag="PKM27_18890"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNR09395.1"
                     /translation="MSRFFQVVILTFILLCGSSFSVMENPQVRSVLAEGEIYKISVDR
                     AGIFKMDYAFLKNELGIAIDQKDPRNIRLYGFGGGMLPETIKNGVDLEMPEIPISVHG
                     EGDGKFDANDYILFYSPGPDQSYWDPADSMFLVRRNLYSRSAVFFLKMGNGPGLRMAA
                     PAPFPTVSTDTLDAFLDLAHFEEEKTNLLAGRTSTYGSGKNWYGDYFKTLRKKDYSGQ
                     FDLSGGIVNSRIKTKIGFAARSDVYTTAILQFYQSKFEIRLNPTNTADVEDDVARETK
                     TTFSLPYQNQPANLVLDYPATVSPSEGWLDFISINVWKPLNFDGSPVIAYHPGNPGGQ
                     SVFRIETQKTGFEVWEVLDSNLPKRLALESRPEGAYFPGKPGGTPGIYVLFDPAGNLP
                     RPNRLGKIPNQNLAALSGAKSVILYHPDFKEAAEKLAAHRASFSGISTEAIAVDEVFN
                     EFGSGQADPTALRNFARHLYLNDPTFEYLTLFGDGSYDYLGLNKELAKNENFIPVFET
                     EESLSPLYAFPSDDYFGLLEEGEGGPDLTGDLDIGVGRIPVRSKEEAGILVDKIIHYD
                     THSDTYGDWKLRVAFSADDEDANIHLEQTESISNQLQASQPLLNLQKIYLDAFKQEAG
                     SGGELIPGANQSLNQNIFQGLLVFNYLGHGGPKGLSQEGLLRNTDVEVWSNKSRLPLV
                     ITATCSFAPYDDPAVPSTGEALFRSPYGGAIALLTTVRNVYSSSNEILTRAVFEQLFV
                     RQADGKPQTLGEVMKKAKNRISGNGFDRLNARKFALLGDPAQHLSLPALQVKTTAING
                     KRVQEGDLDTIRSLQLLSVQGEITDAANRLLSGFNGTVSVTLFDKKSTVKTLGQNSDS
                     YPKSFQVQNSILFKGNARVENGRFEIRFVVPKDINYTFGPAKLSYYASSGEGLEASGY
                     FDRLIAGGSLAQLSDQEAPLVRPYINHPGFRDGGKTHPDPLLLVDLYDENGINATGNS
                     IGHDLVAILDRDSRKQYVLNAFYESAEGDYKRGLVTFPFSDLSPGFHHLEIIAWDVAN
                     NSGRGEITFEVTDPAAGGHILDIQAVPNPAQGSFRLLLVHDLQDLNEAKLKLQVLDYT
                     GRVLFGKEEVLSPSPVIYWSHGFDSGLPAGLYLIQAEIWSGGRRVDVRGTKVLRIP"
     gene            4940..>5601
                     /locus_tag="PKM27_18895"
     CDS             4940..>5601
                     /locus_tag="PKM27_18895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNR09396.1"
                     /translation="MKKALFILFLFSGLLSLAQDPVLSQFYSAPLLLNPAFAGNTLAP
                     RFALNYRNQWPFLQQAYRTYAISYDQFFEQYNSGLGISLLADDAGKGLYKTVKLSGIY
                     AYRLKVGANTYIKTGLEMAGVQAHVNWDKLIFPDQLDPLYGETGPGGSPIPSQELRPD
                     QTNRLYLDLTMGTMIYNPYFYGGIALNHVNSPDVRFIRISDNTSSGLPVRLTIHGGSQ
                     VEL"
BASE COUNT         1319 a         1422 c         1475 g         1385 t
ORIGIN      
        1 aaaattttaa tatgttccta agtctcaact ccttaggcta ctcgtgaact tccctcaata
       61 gtcaatagtc aatagtcaat agtcaatagt taagctgact actcgtttct cgtttctcgt
      121 ttctcgtttc tcgtttctag ctgactactc ctgttcctca aaatcaaaaa tgagcgaaaa
      181 cctcagggta tttgcaagag ggtcccggtt ggttgcggcg cgcgccgtgg gaaccaggta
      241 tgaaagattc atgccgaaca cattgtattt tacgcccagg ccgagggtca gatactggcg
      301 gtttcctttg taggggtttt cgcggaaata accggcccga agcgcaaact ggttattgta
      361 ccagtattcg gtgccgaaag aaaacatcaa ttcctgcaat tcttccttaa agccgttggg
      421 ggcgtctcca aatgacttga accaggagga tacgacgttg tattgtttat aatccgggat
      481 ctggtccccg tttttatcgt aatccggatg ggtggatgca tagggcgacg ggaccagcaa
      541 tttattgatg tccgccgtca gggtgaactt attataatcg tcaaaattcc aggtataggc
      601 cgcgccgatt cccaggttgg ccggaatata ttcctggttg gccttggtat aggaaatttt
      661 ggtgcccagg ttggtcaggg caaggcctgc ggtgaaaaaa ttctggccga ccggtttttt
      721 ataggaaaaa ctgatgtcgg tggcccccgc aatgccgggt ttgatgtcgt tgccgccgat
      781 gttcaggtcc gaagcaagaa aggaatagat aaacttcagg cttaccgcgg cggcaagctg
      841 gtcgctcagt ttacgggaat aggtccctgt cacttcaaat tcattgggtt tgcccgaacc
      901 cagcggtgcg ccgttgtcat cggtataatc gatattcccc agtgaaaaat agcggaggct
      961 cccgccaatg gcctgaaggt ctccgatctt ggtataaccg gcaacatagg ccatgtatac
     1021 gtcggtgagc ccaagagccc tgagccaggg agaataagtc gtggagatcg accttggttt
     1081 ttcggcaaaa gccagcaggg aggcattgtt gtatatggag ccggcatcgg attccacggc
     1141 gatcccggca tctcccattc ctcctgccct cgaatccgga gtaatcctca ggaagggtac
     1201 ggcagtgaca atcgtattga tgcattccct tcccgtcagg gcatccacca tttttccggt
     1261 ggcgggatca tatctgcatt gggcacttaa atcagccagt gcaaaccagg acaggctaaa
     1321 aaggacagcg ccgtaaaatt tcttcatctg aaaatgattt aaaaaatcgc acaaatatac
     1381 gaatacctca ttaatgggct aaaagccaaa ttattacaaa ggttgtctta aggtattctt
     1441 aagactttgg tcccccggac atctaccctc cggcctcccg accagatttc cgcctggatg
     1501 aggtacaatc ccgcagggag cccggaatca aagccatggg accaatagat cacgggggat
     1561 ggggaaagga cctcttcctt gccgaacagg acccggccgg tgtaatccaa tacctgcaac
     1621 ttcaattttg cctcattgag atcctgcaga tcatggacca gcaggagcct gaaactgccc
     1681 tgggctgggt tgggcaccgc ctggatatcc aggatatgtc ctcctgcggc cggatcggta
     1741 acttcgaaag tgatttctcc tcttccggaa ttgtttgcca catcccaggc aatgatttcg
     1801 agatgatgga agcccgggga taaatccgaa aaagggaagg tgaccagccc ccttttatag
     1861 tcgccttcgg ccgattcata aaatgcattc aatacatact gtttgcggct gtcccggtca
     1921 aggatggcta ccaggtcatg gccaatgctg ttccctgtgg cattgattcc attttcgtcg
     1981 taaaggtcta ccagcagcag gggatcgggg tgggttttac ccccatccct gaatccggga
     2041 tgattgatat agggtctgac caggggggcc tcctggtcgg aaagctgggc cagggacccg
     2101 cccgcgatga gccggtcaaa ataaccggaa gcctcgagcc cttcgccgct gctggcgtaa
     2161 taactcagtt tggcggggcc aaaagtataa ttgatgtcct ttgggacgac aaaccggatc
     2221 tcaaacctgc cgttttccac ccgggcattg cccttaaaca ggatgctgtt ctgcacctgg
     2281 aaggatttgg ggtagctgtc tgaattctgg cccagggttt taaccgtgga ttttttatcg
     2341 aataaggtca cggaaaccgt gccgttgaat ccggacagga gacgatttgc cgcatcggtg
     2401 atttctccct gcacggataa aagttgaagt gagcggatgg tgtccaggtc cccttcctga
     2461 accctctttc cattgatggc cgtggttttg acttgcaagg caggcaggga aagatgttgg
     2521 gccggatcgc cgagaagggc aaatttgcgc gcattgagcc ggtcgaagcc attgcccgaa
     2581 atccggtttt tggccttttt cattacctcg cccaaggttt gcggttttcc gtcggcctgc
     2641 ctgacaaata actgttcaaa taccgcccgg gtgagaattt cattggagga agaataaaca
     2701 tttctcaccg tcgtcagaag ggcaatggct cctccataag gactgcgaaa cagggcctcc
     2761 ccggtggaag gcactgcggg gtcatcataa ggggcaaaag aacaggtagc ggtaatgacg
     2821 aggggtaagc gtgatttatt ggaccagact tcgacatcgg tattgcgcaa taggccctcc
     2881 tgggacaagc cttttggacc gccgtgcccc aggtagttaa agaccaggag gccctggaaa
     2941 atattctggt tgaggctttg atttgccccc ggtatcagtt ctcctccgga acccgcttcc
     3001 tgtttaaacg cgtccaggta gattttttga agattcagca ggggttggct ggcctgaagc
     3061 tgattgctga tgctctccgt ttgttcgagg tggatattgg cgtcctcatc atccgccgag
     3121 aaagctaccc tgagtttcca gtctccatag gtatcggaat gggtatcgta atgaatgatc
     3181 ttgtcaacca gaatgccggc ttcttctttt gatctgacgg ggatgcgtcc tacccctata
     3241 tcgaggtccc cggtcaggtc tggcccccct tcgccctcct ccagcaggcc aaaataatcg
     3301 tcggagggga aagcatacag cggggacagg gactcttcgg tttcgaagac cggaatgaaa
     3361 ttttcatttt tagccagttc cttatttaat cccaggtaat cgtaggagcc gtccccgaac
     3421 aaggtcaggt attcgaaggt cgggtcatta agatagaggt gccgcgcgaa gttgcggagc
     3481 gcggtgggat cggcctggcc gctgccgaat tcattaaaaa cctcgtcgac tgcaatggct
     3541 tccgtcgata tgccggaaaa ggaagcccgg tgggcagcga gtttttcggc agcctcttta
     3601 aaatccgggt ggtacaggat tacggatttt gcccctgaga gcgcggccag gttttgattt
     3661 gggattttac ccaagcggtt gggccggggc agattgcccg ccggatcaaa caggacatag
     3721 atgcccggcg tccctcccgg tttgcccggg aaataggccc cttccggacg ggactccagg
     3781 gccagccgtt ttgggagatt ggaatccagg acttcccaga cttcgaatcc tgtcttttgg
     3841 gtttctatcc ggaatacgga ttgccccccg ggattcccgg gatggtaagc aatgaccggg
     3901 gagccgtcga aattcaaggg tttccaaaca ttgatggata taaaatcaag ccagccttca
     3961 ctcggactca cggtggccgg gtagtccagg accaggttgg ccggttgatt ctgataagga
     4021 aggctgaagg tggtcttcgt ttccctggcg acatcatcct ccacatccgc cgtattggtt
     4081 ggattgaggc gaatttcaaa tttggattga taaaattgaa ggatagccgt ggtgtacaca
     4141 tcagaccttg ccgcgaatcc gattttggtt tttatcctgg aatttacaat acctccggaa
     4201 aggtcaaact gaccggaata atccttcttc cggagggttt taaaataatc cccgtaccag
     4261 tttttccctg aaccataggt cgaggtcctg ccggccagca aattggtttt ttcttcttca
     4321 aaatgagcca ggtccaggaa ggcgtcgagg gtatccgtgc ttaccgtcgg gaagggagcg
     4381 ggagccgcca tgcgtaaacc tggcccattg cccattttca ggaaaaaaac ggcggatctg
     4441 gagtacaggt tcctgcgcac gagaaacatg ctgtctgcag gatcccaata ggattggtcc
     4501 ggccccggag aatagaacaa gatatagtcg ttcgcatcaa attttccgtc cccttccccg
     4561 tgaacggaga tcggaatttc cggcatttcc aggtcaaccc cgttcttgat ggtctccggc
     4621 agcatgcccc cgccgaaacc atagagccgg atgtttcgcg ggtctttctg gtcaatggct
     4681 atgcctagtt catttttaag aaaagcgtaa tccattttga aaataccggc gcggtctacg
     4741 ctgattttat agatttcacc ctccgccagt acggaccgga cctgtggatt ttccatcacc
     4801 gaaaaggaac tgccgcacag cagtataaaa gttaaaatca caacctggaa gaaccgggac
     4861 atcgtgttat caaagttaag tctatattta atatcactaa aacgtttaaa cttgtaaacc
     4921 ttatgcgccg gagcggggaa tgaaaaaagc actattcata ttatttctgt tttccgggct
     4981 tttatccctg gcccaggacc cggtgttgag ccagttttac agtgcgcccc tgctcctgaa
     5041 tcccgctttt gccggaaata ccctggcgcc ccgctttgcc ctgaattacc ggaatcaatg
     5101 gccttttctg caacaggctt accgtaccta tgccatctcc tacgatcagt tttttgaaca
     5161 gtataacagc ggcctgggaa tatccctcct tgccgatgac gccggcaaag gtttatacaa
     5221 aaccgtcaaa ctctccggca tatatgccta ccgtctcaag gtcggcgcaa acacctatat
     5281 caagaccggc ctggaaatgg cgggggttca ggcccatgtc aactgggaca aactgatttt
     5341 tccggaccaa ctggatcctt tatatgggga gaccggtccc ggaggcagtc ccatcccgag
     5401 ccaggaactg aggcccgatc agaccaaccg gctttatctt gaccttacga tgggcacgat
     5461 gatctacaat ccctatttct acggaggaat cgccctgaac catgtgaata gcccggatgt
     5521 ccggtttata cggatcagcg acaatacgag cagcggcctt ccggtgcggc tgaccatcca
     5581 cggaggcagc caggtcgagc t
//
LOCUS       DAOQXT010000186         5452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_15263_length_5452_cov_6.263480, whole genome shotgun sequence.
ACCESSION   DAOQXT010000186 DAOQXT010000000
VERSION     DAOQXT010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15263_length_5452_cov_6.263480"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PKM27_18900"
     CDS             complement(<1..878)
                     /locus_tag="PKM27_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR09397.1"
                     /translation="MDINRRDFIQQSAIAAGSLMINPIPSPAARTMAKKRLALVGTGS
                     RGAGFWGKTILDNYKDLVEFVGLCDINPGRLEYAKSYIGADCRTYSDFDLMMKESKPD
                     MVIVTTVDATHHEFIVKALQYGAEVITEKPMTTDEVKCQQILDAEKRSGKKVTVGFNY
                     RHNPHVTKIKELLTNQRVGKLSSVDFHWYLNVYHGADYFRRWHGFINKGGSLWVHKAT
                     HHFDLLNWWLDSDPVEVMAFGQLDHYGKNGAVRGKTCRECDHKATCKFYYDINKNKEY
                     VNLYVKNEHHDGYYRD"
     gene            complement(1252..2220)
                     /locus_tag="PKM27_18905"
     CDS             complement(1252..2220)
                     /locus_tag="PKM27_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR09398.1"
                     /translation="MQKESLNDVQAVDTLGQAYKTLKSEVGKVIIGQDEVVKAVIISL
                     FSNGHSLLVGVPGLAKTLLVSTIAQVLDLEFNRIQFTPDLMPSDITGSEILDEERRFR
                     FNRGPVFANIVLADEINRTPPKTQAALLEAMQERSVTVSGQKHLLPKPFFVLATQNPI
                     EQEGTYPLPEAQLDRFMFNILLDYPSFEEEVVVVKNTTSMHRPELRLVMNSEDISFYQ
                     ELIRKIPIADNVLQYAVNLASKTRPGTSRATEKVNQYISWGAGPRASQFLALGAKVHG
                     AINGKYSPDIEDVQAIAKLVLRHRIVRNYKAEAEGISEDKIIEGLM"
     gene            complement(2446..3936)
                     /locus_tag="PKM27_18910"
     CDS             complement(2446..3936)
                     /locus_tag="PKM27_18910"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR09399.1"
                     /translation="MMIKPLAFLSLLGFAASPTQARILHIGSGHAWTSPSSIQGNVVA
                     GDTVLIHAGSYTGTYFISNLNGNAASPILIKAAPGETVTLSGGSESFHFSDISYVILE
                     DLIIQGQTGNGMNIDDAGSFGTPSHHILIRNCIFRNISASGNNDLLKLSGVDDFEVRD
                     CEFLNGSAGGSGIDMVGCHRGLFQGLQFDQMGSNCIQIKGGSSDITIRRNRFVNGGAR
                     ALNIGGSTDLEFFRPQNATFEAASVEVYANVFEGAQTPFAFVGAVDCKVYNNTIFRPT
                     RWAMRILQETVDPGRFEACGRNTVQNNIFVITTVVTTEVNIGPDTAPNTFIYSNNLWF
                     KEGQSGWSGPVLPVADPAQLVANPQVNATNYMIGSTSPAYQKGKAVSPPVTDYYGKNY
                     KSPPSIGAVEFEGTLALRPAQSKDKKQGQAARVKLSPNPAVSHLRVEAPETAIYGIVD
                     VYGQSWMQGVLQKGENRIGIHHLGAGAYYLVIQFRYYNLSRRWIKL"
     gene            4052..4780
                     /locus_tag="PKM27_18915"
     CDS             4052..4780
                     /locus_tag="PKM27_18915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNR09400.1"
                     /translation="MDKRTFLKTGLLGSLAAPLASWDFPLAAGRTTILFQGDSITDAG
                     RDKAQYYANSASGMGTGYVHMAASTLLEKYPDQGLRIYNRGISGHKVFQLAARWEDDA
                     LNLKPDVLSILIGVNDFWHMLNGQYDGTAAVYENDLRALLARTREALPQVKLILGEPF
                     VVRGGTAVGSPRWNTEFPAYQAACKKVAGEFKAAFIPYQSLFDGVLNKGGAAYWCPDG
                     VHPSLAGARLMANAWVTAYAEVKG"
     gene            5128..>5452
                     /locus_tag="PKM27_18920"
     CDS             5128..>5452
                     /locus_tag="PKM27_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09401.1"
                     /translation="MNRKEMHAHESSANSSRSPTSEVYDHFLNRKARNESRRGAAMKM
                     QSFISSVDSSRAQRPPRFMITFLTANRAMNRKEDPQRNACTRILCGIFALSAPSAVIN
                     HFLNRK"
BASE COUNT         1301 a         1430 c         1361 g         1360 t
ORIGIN      
        1 ctgtcgcggt aataaccgtc gtggtgttcg ttttttacat acagattgac gtattcttta
       61 tttttattga tgtcgtaata aaacttgcaa gtggccttgt ggtcgcattc ccggcaggtt
      121 tttccgcgga ctgcgccgtt tttaccgtaa tggtccaatt ggccgaaagc catgacttcc
      181 accggatcgg aatccagcca ccagttaagc aagtcgaagt ggtgggtggc cttatgcacc
      241 catagggaac cgcccttgtt gatgaaccca tgccatcgcc ggaaataatc cgccccgtga
      301 tacacattga ggtaccagtg gaaatccacg gagctcagtt ttcccaccct ttggttggtc
      361 aggagctctt tgatcttcgt gacatgggga ttgtgccggt aattgaatcc aacggtcact
      421 tttttgccgg atcttttttc cgcgtccagg atttgctgac atttgacctc gtcggtggtc
      481 atcggttttt cggtgatcac ctcagcgcca tactgcaggg ctttgacaat aaattcatgg
      541 tgggtggcat ccaccgtagt gacgatgacc atatccggtt tgctttcctt catcatcagg
      601 tcaaaatcgg aataggtcct gcaatccgcc ccgatatagg atttggcata ctccaggcgg
      661 ccggggttga tatcgcacaa acccacaaat tcgaccaggt ctttataatt atcgaggatc
      721 gttttacccc agaagcccgc gccccggctc ccggtaccta ccaacgcgag cctttttttt
      781 gccatggtcc tggccgcagg acttgggata gggttgatca tcaggctgcc ggcagcgatc
      841 gccgattgtt ggataaaatc ccttctgttg atatccatgg ttgaattttt tggtgtatcg
      901 tccaaagata ataaaaggcc ggcttcattt ggctgcctcg ataaaagtca aataatccgg
      961 aaaatccgga ttctggtggc atgccaacca gaatgtattt ccatcactaa atcatacaga
     1021 tcattccgct ccgaacatta acattcggaa ctccaaacca tgcaacgcat tgttgaaatc
     1081 gtaaaaggaa tttgccatag tcccagaata ctgaaatatg ggtgaacggt aaagggttaa
     1141 gggttaaggg ttaagggtta aaggaccaaa attacaaatg ataaactggc taactgtcca
     1201 actcgctaac ttgcaaactt gctaacttgc taactgataa actggttaac gtcacatcaa
     1261 tccttcaata atcttatcct ctgaaatccc ctctgcctcc gccttgtaat ttctcacaat
     1321 ccggtgacgg aggaccagct tggcgatcgc ctgtacatcc tcaatatccg gggaatattt
     1381 tccattgatc gctccgtgga ctttagcgcc cagggccaaa aactgggagg ctcgggggcc
     1441 tgcaccccag gagatgtact ggttgacttt ttcagtggcg cgggaagtgc ccggacgggt
     1501 tttgctggcc aggttgacgg cgtattgcag cacattgtcc gcaatcggga tcttccggat
     1561 cagttcctga taaaaactga tatcctccga attcatcacc agccggagtt cgggacggtg
     1621 catggaggtg gtgtttttta cgaccacgac ttcctcttca aaagaaggat aatctagcag
     1681 gatattgaac ataaaacggt cgagctgggc ctcgggcagg gggtaggtcc cttcctgctc
     1741 aatggggttt tgggtggcca ggacaaaaaa gggtttggga agcaggtgtt tctggccgct
     1801 caccgtgact gaccgttcct gcatggcttc caaaagggcc gcctgggtct tggggggagt
     1861 ccggttgatc tcatcggcca gcacgatatt ggcaaagaca ggcccccggt tgaaccggaa
     1921 ccttctctcc tcatccagga tttcggaccc cgtaatgtcg ctgggcatca ggtcgggagt
     1981 aaactggatc cggttgaatt ccaggtccag gacctgggca atggtggata ccaggagggt
     2041 tttggccagg ccgggaacac cgaccagcag gctgtggccg ttggaaaata aagatatgat
     2101 caccgctttc accacttcgt cctgaccgat gatcactttg cccacttccg atttgagtgt
     2161 tttatatgcc tggcccaggg tgtctaccgc ctgaacatca ttcaacgatt ctttctgcat
     2221 aactaatgat tgcgcacaaa aatagcataa ttagagcagg gtgtaagatt tgggaggagg
     2281 gggcagcatt aaggaaatgt tatcggtcgg gccacccggt gaccggtatc tgttttgcca
     2341 agaataaaat ctgctggatt atcgcttctg gaggacctgg ctcagtccac aatcgctaag
     2401 ccgcacaaaa acccttaatt cccttaattc ctttaattct cttaattaca atttaatcca
     2461 tctccggctc aggttgtaat accggaattg aatgaccaga taataagcac ccgcccccag
     2521 gtgatgaatg ccaatccggt tttccccctt ttgaagcacc ccctgcatcc aggattgacc
     2581 ataaacgtcc acaattccgt aaatcgccgt ctcaggcgcc tccaccctca ggtgacttac
     2641 cgcggggttg ggagataatt tgacccgggc agcctggcct tgctttttat ccttggactg
     2701 cgccggccta agggccaggg tgccttcaaa ttcaaccgca ccgatggacg gcggcgattt
     2761 ataatttttg ccgtaataat cggtcaccgg cggactcaca gcctttcctt tctggtatgc
     2821 cggactggtc gaaccgatca tataattggt ggcattcacc tgcggattgg ccaccagttg
     2881 ggccggatcc gccaccggaa gcaccggacc tgaccatccg gactgccctt ccttaaacca
     2941 gagattattg gaatagataa aagtattggg tgcggtatcc gggccgatat tgacctcggt
     3001 ggtcaccacg gtggtgatca cgaaaatatt attttggacc gtattgcgcc cacaggcttc
     3061 gaaacgaccg ggatctacgg tttcctgcag gatgcgcatg gcccagcggg tcggcctgaa
     3121 aatggtattg ttatacactt tgcaatccac cgcgccgaca aatgcaaaag gcgtctgggc
     3181 tccttcgaat acattggcat aaacttcgac gctggccgct tcaaaggtcg cattctgggg
     3241 acggaaaaac tccaggtcgg tgctgccccc gatattgagg gcacgggcgc ccccgttgac
     3301 aaagcggttt ctccggatgg tgatatcgct gctcccccct ttgatctgta tacaattgct
     3361 gcccatctgg tcaaactgaa gtccctggaa aagaccccgg tggcatccga ccatatcgat
     3421 gccgctgcct ccggccgacc cattcaaaaa ctcgcagtct ctcacctcaa aatcatccac
     3481 cccggacaat ttaaggaggt cgttattgcc tgaggcgctg atattccgga aaatacaatt
     3541 acggatgagg atatgatggg aaggagtccc aaaacttccg gcatcgtcga tattcatgcc
     3601 gttgccggtc tgcccctgga tgatcaggtc ttccaggatc acataagaga tgtcggaaaa
     3661 atgaaaggat tcactccctc ccgatagcgt tacggtttca cccggtgccg ctttgatcag
     3721 gatgggtgag gccgcattgc cgttgaggtt ggaaataaaa tacgtaccgg tataactgcc
     3781 tgcatgaatg agcacggtat cccctgcgac cacatttccc tggattgaag atggggaggt
     3841 ccaggcatgt ccactcccga tatggaggat acgggcttgc gtgggggagg ctgcaaaacc
     3901 aagcaaggac aggaaagcca ggggtttgat catcatatcc attttgatta attcttatct
     3961 aaaccggaat ctgcctccgg aaattgtgaa aaatactgtt aaagaaactt cctaacttgc
     4021 caacttacta acttgctaac ttgccatcga tatggataaa cgaacctttc tgaagaccgg
     4081 gctccttgga agcctggctg cacccctggc ctcctgggac tttcctttag ctgcaggcag
     4141 gactaccatc ttgttccagg gagacagcat caccgatgcc ggccgggaca aagcccaata
     4201 ttatgccaac agcgcttcgg gcatgggcac cggatatgta catatggctg cctccaccct
     4261 gctcgaaaaa tatccggacc aggggctccg catttacaac cgtgggatct cgggacataa
     4321 ggtattccaa ctggccgccc ggtgggaaga cgatgccctc aacctaaaac ccgatgtgct
     4381 cagcatactc atcggggtca atgatttctg gcatatgctc aatggccagt atgatggtac
     4441 agctgcggtt tatgaaaacg atctgagggc cctcctggca aggaccaggg aagccctgcc
     4501 ccaggtgaaa ctgattctcg gcgagccttt tgtggtccgg ggcggaaccg ctgtcggaag
     4561 cccaaggtgg aataccgagt ttcctgccta ccaggcggcc tgtaaaaaag tggccggtga
     4621 attcaaagcg gcctttatcc cctatcaaag tttgtttgat ggggtcctga ataaaggagg
     4681 tgcggcttat tggtgccccg atggggtgca tccttccctg gcaggtgcca gactgatggc
     4741 gaatgcctgg gtgactgctt atgctgaagt gaaagggtaa cctcgagtca ccccctctct
     4801 tcaagtaaaa gaaatgcaat cctttcctct gcggattctt cgcgccctca gcggccttcg
     4861 cggtttttga tctttttctc aaccgcaaag cttacaatga atcgtgaaga ggggccgcaa
     4921 tgaaaatgca atcattcatt tcctctgcgg aatcttcgcg ctctcggcgg cctcctcggt
     4981 ttatgatcac ttccttaacc gcaatgcgtg ccatgaatcg cgaagggagc cgctaagaaa
     5041 atgcaatcat aaatctcctc tgcggattct tcgcgatctc ggcgacctct gcggttaata
     5101 atcactttct taaccgcaaa gcgcgcaatg aatcgcaaag aaatgcatgc acacgaatcc
     5161 tctgcgaatt cttcgcgctc tccgacctct gaggtttatg atcacttcct taaccgcaaa
     5221 gcgcgcaatg aatcgcgaag aggagccgca atgaaaatgc aatcattcat ttcctctgtg
     5281 gattcttcgc gcgctcagcg ccctccgcgg tttatgatca ctttcttaac cgcaaatcgc
     5341 gcaatgaatc gcaaagagga tccgcaaaga aatgcatgca cacgaatcct ctgcggaatc
     5401 ttcgcgctct cagcgccctc tgcggttatt aatcactttc tcaaccgcaa ag
//
LOCUS       DAOQXT010000187         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_16094_length_5234_cov_7.299479, whole genome shotgun sequence.
ACCESSION   DAOQXT010000187 DAOQXT010000000
VERSION     DAOQXT010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16094_length_5234_cov_7.299479"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1429)
                     /locus_tag="PKM27_18925"
     CDS             complement(134..1429)
                     /locus_tag="PKM27_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09402.1"
                     /translation="MRVKGRYSLIALSVLVLIQAFFIRQDQFLASILLFGAGFGFYLL
                     AGTARLQDDPITNRLNLFLKIILLFAFPQGSDDLYRFFWDGHLLLQGFHPYALRPDQV
                     LGQLEPFQEVIYDHLNSPAYYSVYPPPLQFLFGLCALASFKSIFLFSIGWKFLLLLAD
                     QWLLRYLTKFPHLDPGTAFWYRWNPLILSEIAGNGHPEGLMIPALVLSLYLISHQRTA
                     LSSFTLALAAAIKLFPIALLPSFARHLGFRKAISHIPLTLMVFVVLIFPVWPHGDHFF
                     SSLKLYFQKFEFNGSFYEIWKSIEFRRHGHNNIAFIGKMIPVLFVAFAGFVLILQRPN
                     HFQSLIRSCLLIWTGYLLLSTTVHPWYVLPLIPLGLLSGFIFPLAWSGAVFLSYSWYD
                     PGLGMGWKGAFQAMEYAILAGAVVVDLVKFRKQGLEAKS"
     gene            complement(1435..2913)
                     /locus_tag="PKM27_18930"
     CDS             complement(1435..2913)
                     /locus_tag="PKM27_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR09403.1"
                     /translation="MIILAYILAAIYILALVFITVYAILEFQLLYYYLKKYKKIRAYT
                     PPALKDWPMVTVQLPVFNEMYVIDRLIDDICQIDYPREKLEIQVLDDSTDETREIIHK
                     KCAHYAARGFDIKHLHRNNRAGYKAGALKEGLASAKGEFIAIFDADFLPRPDFLKKCI
                     PYFQDPKIGVVQTRWDHINEGYSLLTMLQAFQLNVHFTIEQSGRQSGDYFLQFNGTAG
                     TWRKKAIEEAGGWHTDTLTEDLDLSFRAQLKGWEIYYLQEIGSPSELPAEMNGIKSQQ
                     ARWMKGGAEVARKLIPVIWASKLDFWQKVHAIMLLMGSSIFLVVLALALTSVPLAFLL
                     VRFGIPAKLFAFGLAGTLSILLVHYTANVIIAWQNRNPLISFLRFIFLFPLFLTFSMG
                     LSLHNSVAVIQGWRGKKSPFIRTPKYNIRNIRDRISNHKYLSHRLSWITVFEGLLATY
                     FFYAFYYGMRTHQDAFLLFHLALGLGFSGIFLFTLYHLSLKN"
     gene            complement(2918..3625)
                     /locus_tag="PKM27_18935"
     CDS             complement(2918..3625)
                     /locus_tag="PKM27_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR09404.1"
                     /translation="MIPLRPVMAITDVIIPAFNEEKSIGLVLKEIPQDKVRQVVVCNN
                     HSTDRTREVALAHGAMVVDEMNKGYGYACLRAMEHIRHSRPEPDLIVFLDGDHSDYPE
                     QLPELTAPILHDGYDLVIGSRALGHPEKGSMTLPQRFGNWLATHMIHWLYGYRFTDLG
                     PFRAIRYKKLLELNMCDKTYGWTVEMQVKAAKLGFRCTEVPVKYRRRIGVSKVSGTIK
                     GTILAGFKIIWTILKLL"
     gene            3680..5179
                     /locus_tag="PKM27_18940"
     CDS             3680..5179
                     /locus_tag="PKM27_18940"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR09405.1"
                     /translation="MIFRARTFPVLKYLVVTFLAFWLGGCYRERFYEEGDAILRFSVD
                     TLRFDTVFTRVGSATRSFKVFNDQSLPVKVASIKLQQGLSSVFRLNVDGQAAPVKELQ
                     GIEIGAGDSIYLFLEVTIQPDQPLSVSPFVIEDKILFNTNGKEQQVMLEAWGQNANYI
                     PSRYSQGGIALLTCDLLDETWDDPKPYVIYGILAVDSCTLRIPAGARVYVHGGLVRNS
                     GNIYNDGILYVLDQGKLLVEGTKERPVIFSTDRLEPELKEEWGAWSGIRFGPGSRGNR
                     INYAEIRSAFVGLWADSSSEVTVRNTRIYNTASSGIVGVHARVIAENCLFHSNGGHGV
                     QFAFGGNYRLEYCTLASYDNDAEALSMNNAILRDPETRRIELFPLIATVRNCILFGSG
                     KDELDLSDLTDPKDPLFFQYSFDHCYIKAEELLAEKNYPKFFEKCASCIQAQSSDTVF
                     LNVAKLDFRLDTFSVVNDKARPLEHIPFDLLGVKRDAGTPDIGCFELLQ"
BASE COUNT         1340 a         1268 c         1407 g         1219 t
ORIGIN      
        1 cgggcaaccc attgtccata taatactaat attattaaat aatcattaca ctagggcgta
       61 ttggccgggc atttccggga gttttagtgg tccagcccct agcttccagc tcccagcttc
      121 cagctcccag cttctagctt ttagcttcca gtccttgttt cctgaatttt accaaatcca
      181 ccacaacagc tcctgccagg atggcatact ccattgcctg gaaagccccc ttccaaccca
      241 tacccaggcc ggggtcgtac caggaataag acagaaaaac ggcccccgac caggccagcg
      301 gaaatataaa tccggacagc agtcccagtg ggatcagggg caagacatac cagggatgta
      361 ccgtcgtgct gagcaggagg tatccggtcc aaatcaggag gcaggatcgg ataaggcttt
      421 ggaaatgatt gggccgctga aggatcagaa caaagccggc gaatgcaaca aacaggacag
      481 ggatcatttt accgataaac gcgatattat tgtgcccatg cctcctgaat tcaatggact
      541 tccagatttc ataaaagctg ccgttgaatt caaatttttg aaaatacaat tttaaagagg
      601 aaaagaaatg atctccatga ggccagaccg gaaagatcag cacgacaaag accatgaggg
      661 taaggggaat atggcttatg gccttccgaa aaccgaggtg acgcgcgaat gagggcagga
      721 gggcgatggg aaacagtttg attgcggctg cgagggcgag ggtgaaagag gacaaagcgg
      781 ttctttggtg cgaaataaga taaagggaaa ggaccagggc cgggatcatc agcccttcgg
      841 gatgtccgtt gcctgcaatt tcggaaagga tcagcggatt ccacctgtac cagaaagcgg
      901 tccccggatc cagatgagga aacttggtga ggtaccggag taaccattgg tcagccagca
      961 gcagcaggaa tttccacccg atggagaaaa ggaaaatgga tttaaagctg gccagggcac
     1021 agaggccaaa aaggaattgc agcgggggtg gatatacgct gtaataagcc ggagaattca
     1081 ggtggtcgta aatcacctcc tggaagggct ccagttgtcc aagcacctga tccggccgga
     1141 gggcataggg gtggaagccc tgcaacaaca ggtggccatc ccaaaagaaa cggtaaagat
     1201 catcagaccc ctgagggaaa gcaaacagga ggattatttt cagaaataag ttcaacctgt
     1261 ttgtgattgg atcatcctgg agccttgccg ttcccgccag caggtagaat ccgaaacctg
     1321 cgccaaagag gaggatggag gccaggaact gatcctgccg gatgaaaaaa gcctgaatga
     1381 gcaccagtac actgagtgcg atcaggctat atcggccttt gacccgcata tttttcagtt
     1441 tttgaggctc aaatggtaga gcgtaaacag gaagatgccg ctaaaaccca atcccagggc
     1501 caggtgaaag aggaggaagg catcctggtg ggtgcgcatg ccataataaa acgcgtaaaa
     1561 aaaatacgta gccaatagcc cttcgaacac ggtgatccag gacaggcggt gggacaggta
     1621 tttgtgattt gaaatccggt cccggatatt gcggatatta tattttggag tgcggataaa
     1681 tgggcttttt ttccccctcc aaccctggat tacggccaca ctgttgtgaa gggaaagccc
     1741 catggagaag gtgaggaaga gcgggaacag gaaaataaaa cgtaaaaaag aaatcagcgg
     1801 attgcggttt tgccaggcga taatcacatt ggcagtataa tgaaccagga ggatggacag
     1861 ggtacccgcc agcccgaaag caaacagttt tgcggggatg ccgaaacgga ccagcagaaa
     1921 agccaaaggg acactggtaa gggccagcgc cagcaccacg aggaaaatgg agctgcccat
     1981 cagcaacata atggcatgca ctttttgcca aaaatcgagt tttgaagccc agatcaccgg
     2041 gatcagcttg cgtgccacct cggcgccacc tttcatccac ctggcctgct gggacttgat
     2101 cccattcatt tcagcaggta attcagaggg agaaccgatt tcctgcaaat aataaatctc
     2161 ccatcctttt aattgggccc ggaaggaaag gtccaggtct tcggtgaggg tgtcggtatg
     2221 ccatcctccg gcctcttcga tggccttttt tctccaggtg ccggccgtgc cgttgaattg
     2281 cagaaaataa tccccggatt gccgtcccga ttgttcgatg gtgaaatgaa cgtttagttg
     2341 aaaagcctgc aacatggtca gcagggaata cccttcgttg atatggtccc accgggtttg
     2401 caccacaccg attttcgggt cctggaaata agggatgcat tttttcagaa aatctggacg
     2461 cggtaaaaaa tccgcgtcaa agatggcgat gaattcgcct ttcgcgctcg caaggccttc
     2521 cttgagcgcg ccggccttgt aacccgcgcg gttgttccga tgcaggtgtt tgatatcgaa
     2581 acccctggct gcataatgtg cgcatttctt atggatgatc tccctggttt cgtcggtact
     2641 gtcatccagc acctggatct ccagtttttc ccgcggataa tcgatctggc agatatcatc
     2701 gatcagccgg tcgatgacat acatttcatt aaaaacgggc aattgaacgg tgaccatagg
     2761 ccagtccttc agggccggag gggtgtaggc gcgtattttt ttatattttt tcaggtaata
     2821 atacaaaagc tgaaattcaa ggatggcata tacggtgatg aaaacgaggg ccagaatata
     2881 gatggcggcc agtatatatg caaggatgat cacgccctca cagcaatttt aatatcgtcc
     2941 agataatctt gaaccctgct aatatagtac cttttatggt gccgctgacc ttggatacgc
     3001 cgatcctgcg acggtacttc accggcactt cggtgcaacg gaaacccagt ttggccgcct
     3061 tcacctgcat ctctaccgtc catccgtagg tcttatcgca catgttcagt tccagaagtt
     3121 ttttataccg gatggcccgg aaaggtccca ggtcggtaaa ccggtatccg tagagccagt
     3181 ggatcatatg ggtggccagc cagttgccaa aacgctgagg aagggtcatg gagccttttt
     3241 caggatgtcc gagggccctg gacccgatca ccaggtcata accatcgtgg aggatgggtg
     3301 ccgtgagttc aggcaactgc tccggataat cgctgtggtc accgtccagg aatacgatca
     3361 ggtcgggttc gggccggcta tgccggatgt gttccatcgc ccgcaggcag gcataaccat
     3421 agcctttgtt catttcatcc accaccatgg ctccgtgggc cagggccact tcccgggttc
     3481 ggtccgtgga atggttgttg cagaccacca cctgccggac tttgtcctga ggaatttcct
     3541 tcagcaccaa tccaatggat ttttcttcgt tgaaagccgg gatgatgaca tcggtgatgg
     3601 ccatgaccgg gcgtaaaggt atcaagacct gtcacaaaat ccggcggacc cccttcctca
     3661 aatggattat ttttgcggca tgatattcag ggcccggact tttccggttt tgaaatacct
     3721 ggtggttacc ttcctggctt tttggctcgg gggctgttac cgggaacgct tttatgagga
     3781 gggagacgcg atcctcagat tttcagtgga tacccttcgt ttcgacacgg tattcaccag
     3841 ggtgggttcg gccacccgtt catttaaagt gttcaatgac cagtccttgc ctgtcaaggt
     3901 ggccagcatc aaactgcagc aggggctgtc ctccgtattc cggctcaatg tggatggcca
     3961 ggccgctcct gtaaaggaat tgcagggaat tgaaataggg gcgggagaca gcatctacct
     4021 ttttcttgaa gtgaccattc agcccgatca gcccctgtcg gtaagcccat ttgtgattga
     4081 ggacaagatc ctgtttaata ccaatgggaa agaacagcag gtcatgctgg aagcctgggg
     4141 tcagaatgcc aattatatcc cctcacggta ttcccaggga ggcatcgccc tgttgacctg
     4201 cgatttgctg gatgaaacct gggatgaccc caaaccctat gtgatttacg ggatcctggc
     4261 ggttgacagt tgtaccctgc gcattccggc cggcgcgagg gtctatgttc atgggggatt
     4321 ggtccgaaat tccggcaata tatacaacga cgggatactc tatgtcctgg accagggaaa
     4381 gctcttggtg gaaggcacca aagaaagacc ggtgattttc tccacggacc ggttagaacc
     4441 cgaattaaaa gaggaatggg gggcttggtc aggcattcgc tttggtcccg gcagcagggg
     4501 caaccggatc aattatgccg aaatcaggtc cgcttttgtg gggctatggg ccgacagttc
     4561 cagcgaggtg acggtccgga ataccaggat ttacaatacc gcgagttcag ggatagtggg
     4621 agtgcatgcc cgggtcatcg cggagaattg cctcttccat tccaatggtg gccacggcgt
     4681 acagtttgcc tttgggggca attaccgttt ggagtattgc accctggcga gctatgacaa
     4741 cgacgcggaa gctctttcca tgaacaatgc catactgagg gatcccgaaa cccggcggat
     4801 tgaacttttt ccgctgattg cgacggtccg taattgcatc ctctttggtt cgggcaagga
     4861 tgaactggac ctgtctgacc tgaccgatcc gaaagaccct ttgttttttc aatactcctt
     4921 cgaccattgt tatatcaaag ccgaagaatt gctggctgaa aaaaattatc ccaagttttt
     4981 tgaaaaatgc gcgagctgca tccaggctca atcgagcgat acggtcttcc tgaatgtggc
     5041 aaaacttgat ttcaggctgg ataccttttc cgtggtcaat gacaaggccc gccccctcga
     5101 acatattccc tttgacctgc tgggcgtaaa gcgggatgcc ggcacacccg atatagggtg
     5161 cttcgagctt ctacaataag ttaaactatt gttatttaat tagttatata gtttaacatc
     5221 cattaacatc atat
//
LOCUS       DAOQXT010000188         5140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_16452_length_5140_cov_5.903048, whole genome shotgun sequence.
ACCESSION   DAOQXT010000188 DAOQXT010000000
VERSION     DAOQXT010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16452_length_5140_cov_5.903048"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="ricT"
                     /locus_tag="PKM27_18945"
     CDS             complement(<1..940)
                     /gene="ricT"
                     /locus_tag="PKM27_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNR09406.1"
                     /translation="MACAGCSTGSKDKPSGCKSNGGCSTGGCNRMNTYDWISVLEIDD
                     PLPFPWVEVSFKNGSHKEFFRKDSHHEIETGDWVVTETASGYDVGKVTLSGELVRLQM
                     KKKNIAEDRVIYKIIRPASDRDIEHMHQARNMEKLALVKGRVIARSLGLDMKLGDIEY
                     QADLRKATFFYTADRRIDFRELVKLYAREFRVKIEMRQIGARQETARIGGIGSCGREL
                     CCSTWLSDFKTVVTSAARYQNLAINQAKLSGQCGRLKCCLNYELDVYMESLDRFPMDI
                     ESIRTSLGTANLLKTDIFRGIMYYELESTGKNKEAQV"
     gene            complement(965..2095)
                     /locus_tag="PKM27_18950"
     CDS             complement(965..2095)
                     /locus_tag="PKM27_18950"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09407.1"
                     /translation="MTFSEIPGLSIEKENILFLIRQGRLPHALLIYGPEGGGKLALSL
                     ALCQYLQCEKPGPSDSCGLCSPCQKSRKLVHPDTHFSFPVSGTNVLSDQLLQEWRQAV
                     IQNPYLNISMWQEKSEQENKLSNISAAECRNMLKKLSLTIYEGKIRILLVWLPEYLGK
                     EGNMLLKLIEEPPPDSLILLVAENRDLLLPTILSRCQSLGIPAFRDDDVQFMVQQSGI
                     QDAGLARNIVRTAEGNMMEALRMVADQVHPHSEKLVRWLRLCYKGDAAEILSWVESFS
                     VQGRDVHRQFLKFGLRFIGQVLSYKISPEEEDTFNEDEWKGIKGLAARLEIGEIQDLA
                     TLFDEHIYFVERNANPKILFTALSLNLRDVLNHREIKTIHLI"
     gene            2252..2662
                     /locus_tag="PKM27_18955"
     CDS             2252..2662
                     /locus_tag="PKM27_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNR09408.1"
                     /translation="MKAAIYRWGHFNAAHRLYNPKWSEEKNKEVFGICTNPNYHGHNY
                     EVVVKVIGEVDPETGFVINLFDLKNLIEKEIEDRFDHKNLNLDCPEFRELLPTAENVC
                     YVIWTLLRNKLDEKYNILVRLYETQRNFVEYPAE"
     gene            complement(2663..3238)
                     /locus_tag="PKM27_18960"
     CDS             complement(2663..3238)
                     /locus_tag="PKM27_18960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNR09409.1"
                     /translation="MAVKSTDLDVAAKASLKNNSDDTLLLKWTRKIENITQGWSSAVC
                     DKNFCYVPTVDTMHLLLAPGEISNMDVHVYPMGIVGTARVTLKIEQMGKPENTLTGTY
                     LFNITTPVHASDRDAIQLYPNPAMEYFHVNAPVVLGKIELYNITGEKAKVFYAYKNKR
                     YYIDDIPSGIYLVRLLNLQEEIVKTLVLKKK"
     gene            complement(3596..3985)
                     /locus_tag="PKM27_18965"
     CDS             complement(3596..3985)
                     /locus_tag="PKM27_18965"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR09410.1"
                     /translation="MRLITYNPFLTRWADDTGAAKSVSVNIVEKDNGFYVDLVSPGFD
                     KSDFKIELHKNQLTVSAEKKNETETRENDKIWRREYTLNSFKRSFHLPETVEGDAIEA
                     NYEQGILKLYIPKKAEAIPAVKTIEIA"
     gene            complement(4006..4437)
                     /locus_tag="PKM27_18970"
     CDS             complement(4006..4437)
                     /locus_tag="PKM27_18970"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR09411.1"
                     /translation="MYKNFKLPFLHPELEQAVQDFVKVWNVDNTKDSGWAWTRPLANI
                     EESDTHFQIVLAAPGLQKNDFKMTLDKEVLTVSVDKEKLETGNKKVVTEFSYHKFTRR
                     FRISPEVDQQGIHASYENGILTITLPKKEEVIRGPKTININ"
     gene            complement(4661..>5140)
                     /locus_tag="PKM27_18975"
     CDS             complement(4661..>5140)
                     /locus_tag="PKM27_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNR09412.1"
                     /translation="KTTFHLVKPGETMESISQLYGIQVKYLYKRNRIETGSQPAVGSK
                     IYLTRKSKFAPNIASPETMENPLPAETPMIHPAPAPQPSPIPLPTENDNKTIQYVRTP
                     LEQSLNGRESHPEFYTVQEKDTLFGIARKFNMSVSSLKELNQLTTDTIQIGQQLKIK"
BASE COUNT         1216 a         1151 c         1339 g         1434 t
ORIGIN      
        1 aaacctgggc ttctttattt tttccggtgg attccagttc gtaatacatg attccgcgga
       61 agatgtctgt cttgagtaaa ttggcggtgc ccagggaagt acggatcgat tcaatgtcca
      121 tcgggaaacg gtccagggat tccatataca catccagctc ataattgagg cagcatttca
      181 ggcggccgca ctgacccgaa agtttcgcct ggttgatggc gaggttttgg tatcgggcgg
      241 ctgaggtgac tacggtttta aaatcggaca gccaggtcga acaacacaat tccctgccgc
      301 aggagccaat gccgccgata cgcgcggttt cctgcctggc tccgatctgc ctcatttcaa
      361 ttttgaccct gaactcccgg gcataaagtt tcaccagttc cctgaaatca atgcgccggt
      421 cggcagtgta gaagaaggtg gctttgcgca ggtcagcctg gtactcaata tctcccagtt
      481 tcatgtccag tcccaggctg cgggcaatga ccctgccctt gaccagggcc agtttctcca
      541 tgttccgggc ctggtgcata tgttctatat cccggtcaga agcgggtcgg atgattttgt
      601 agatgacgcg gtcctcggca atgttctttt tcttcatctg caagcgaacc agttccccgc
      661 tcagggttac cttgcccacg tcatagccgg aggcggtctc cgtcaccacc cagtccccgg
      721 tttcgatttc atgatgggaa tcctttcgga aaaattcttt atgagagccg tttttaaaac
      781 tgacttccac ccatggaaag ggcaggggat cgtcgatctc cagcaccgag atccagtcat
      841 aggtattcat tctgttacag ccgccggtcg aacaacctcc gttgctttta caccccgaag
      901 gtttgtcttt tgatcccgta ctgcatcctg cacaagccat atcgcaaatt tacatcttga
      961 gtgttcaaat gaggtgaatt gtttttattt ccctgtggtt caacacgtca cgaagattga
     1021 gcgagagggc ggtgaaaagg atttttggat ttgcatttct ttccacaaaa tagatatgtt
     1081 catcaaatag ggtggccagg tcctggattt caccaatttc cagccgggcc gcgagtccct
     1141 tgatgccttt ccattcgtct tcgttgaagg tatcctcctc ttccggagag atcttatagc
     1201 tcaggacctg gccgatgaac cggagcccga atttcagaaa ctgccggtga acatcccggc
     1261 cctgaacaga aaaactctcc acccaggaca ggatttctgc cgcatcccct ttgtaacaaa
     1321 gacgcagcca gcggaccagt ttttcactgt gcgggtgaac ctggtcggca accatgcgca
     1381 gggcttccat catattccct tccgccgtgc gcacgatgtt ccgggcaagg ccggcgtcct
     1441 ggatcccgga ctgctgcacc atgaactgga catcatcatc ccggaaagcc ggaatcccca
     1501 gggattgaca acgggacagg atggtgggga ggaggagatc ccggttttcg gcaaccagca
     1561 ggatcagact gtccggtggg ggttcttcga tcagtttcag caacatattg ccttctttcc
     1621 caaggtactc gggaagccag accagcagga tgcggatttt tccctcgtag atggtcagcg
     1681 acagcttctt caacatgttt ctgcattccg ccgcactgat attggataat ttattttcct
     1741 gctccgactt ttcctgccac atggaaatat tgaggtaggg attttgaatg acggcctggc
     1801 gccattcctg gagcagttgg tcggataaga cattggtccc ggataccggg aaagaaaaat
     1861 gggtgtcggg gtgaaccaat ttgcgggatt tctggcaggg gctgcacagg ccgcaggaat
     1921 ccgagggtcc cggtttttca cattgcaggt actggcacag ggcaaggctg agggccagtt
     1981 tacctcctcc ttccgggcca tagatcagga gggcatgggg cagtcggccc tgccggatta
     2041 aaaacaggat attttctttt tcaatgctga ggccgggaat ttctgaaaag gtcatattga
     2101 atccaaatgc aagaaacgga atccccccga catttgacct gcattggttg aaggggattc
     2161 ccagcggtct atggcttaaa ggggggtatg ggcatattcc cggggatatg agcggaaagt
     2221 ttaggaactt tgccggtgaa aaaagaaaaa aatgaaggca gcgatatacc ggtggggcca
     2281 ttttaatgcc gcccacagat tatacaaccc caaatggtcg gaggagaaaa ataaagaggt
     2341 ttttggcatt tgtaccaatc ccaattacca tgggcacaat tatgaagtgg tggtcaaagt
     2401 gatcggcgag gtggacccgg aaaccggatt tgtgatcaac ttgttcgacc tgaaaaacct
     2461 gattgaaaaa gaaattgaag accggtttga ccacaaaaac ctcaatttgg attgccccga
     2521 gttcagggaa ctcctgccca ccgctgaaaa cgtgtgttat gtgatctgga ccttattgag
     2581 gaataaactg gatgagaaat ataatattct cgtcaggtta tatgaaacgc agcgaaattt
     2641 tgtggagtac cccgcggaat gattattttt tcttgaggac cagggttttg acgatttctt
     2701 cctgcaggtt caggagccgg accagataga tgccagaagg gatgtcgtcg atataatatc
     2761 ttttgttttt gtaggcatag aagaccttgg ctttttcgcc ggtgatgttg tacagttcaa
     2821 tcttgcccag gaccacgggg gcgttgacat gaaaatattc catggccggg ttgggataga
     2881 gctggatggc atcacggtct gaagcatgga ccggtgtggt gatgttgaag agataggtgc
     2941 cggtcaaggt gttttccggc ttgcccattt gttctatttt aagggtcacc ctggcggtac
     3001 ctacgatccc catgggatag acgtgaacgt ccatattgct gatttcaccg ggcgccagga
     3061 ggaggtgcat ggtgtcgacc gttgggacgt aacagaagtt tttatcgcac accgcgctgg
     3121 accagccttg ggtaatattc tcgattttcc tggtccattt gagcaggagg gtgtcgtccg
     3181 aattattttt gagggaggct ttagcggcca catccaggtc ggtggattta acagccatac
     3241 tgctgggatt gggccccagg ctaagggtga cctggcccgc caaccggctc acctgcaata
     3301 agcataatat caaaatcaat ggcttcaatg ggtactcgtt gattacatat taaacaaaaa
     3361 tataacgctg ttaacaattt ttaccagcgg gtcctgttaa aacttcaaat atttttttat
     3421 catattaatg tattaacaat tattcgttgc cctggtgtta aaacatgcat aagataacag
     3481 aaatggtgga catgcgggcg actttcggcc atatgccggt acggccaggg ccgaacaaaa
     3541 aaagggatcc tgaaattcag gatccctttt gatgatttta aaccgggaat cggatttagg
     3601 cgatttcaat ggtttttacg gcaggaatgg cttcggcttt tttgggaata taaagcttga
     3661 ggatcccctg ttcgtaattg gcttcgatgg cgtcgccttc aacggtctcg ggcaggtgga
     3721 aactcctttt gaaagaattt agcgtgtatt ccctgcgcca tatcttgtcg ttttccctgg
     3781 tttccgtttc atttttcttt tcggcggaaa ccgttaactg gtttttatgc aattcgattt
     3841 taaaatccga tttgtcaaaa cccgggctca ccaggtctac gtaaaatcca ttgtcttttt
     3901 caacgatatt gaccgatacg gatttagccg cgccggtatc gtcagcccag cgggtcagga
     3961 aaggattata tgtgattagt ctcatggtta aaagttattt atgcgttagt taatattgat
     4021 cgttttgggt ccccggatca cttcctcttt tttgggcagg gtgatcgtca ggatgccgtt
     4081 ttcgtaagag gcatggatgc cttgctgatc gacctccggg ctgatcctga aacggcgggt
     4141 gaatttgtga taagaaaatt ccgtaaccac ttttttattg ccggtttcca atttttcctt
     4201 gtccacagag acggtaagaa cctctttgtc cagggtcatt ttgaaatcgt ttttctgaag
     4261 acccggggcg gccaggacga tttgaaaatg ggtgtcggat tcctcaatat tggccaacgg
     4321 acgggtccag gcccagccgg aatctttggt attgtctacg ttccagactt ttacaaaatc
     4381 ctgaacggcc tgctccaact cagggtgcag aaatgggagt ttgaaatttt tatacataat
     4441 atcactttaa gcaatattga tcaaaccaca taccattgaa aaatatgcgc caaaatggct
     4501 tatatttcct ataagtcatg ccattatgac gtaatattcc cgtttaccgg aaaaactggc
     4561 aaatgattca ttaaaaaagg ccttcactgg gggtggaggc ctgaggttgg tgagaaaata
     4621 cctttcgtct tgggaaaggc attaatcttc tatagcgggg ttattttatt ttcaattgtt
     4681 ggccgatctg gatggtatcg gtagtcagtt ggttcagttc tttcaggctg cttacgctca
     4741 tattgaattt ccgggcgatc ccaaaaaggg tgtctttttc ctggacggtg taaaattcag
     4801 ggtgggattc cctgccattg agggattgtt ccagcggggt tcttacatat tgtatggttt
     4861 tattgtcatt ttcggtggga agggggatgg gggagggctg cggtgccgga gccgggtgga
     4921 tcatcggggt ttcagccggg agcgggtttt ccatagtttc agggctggcg atattcgggg
     4981 caaattttga ttttctggtt aaataaattt tggagccaac ggcgggttgg ctccctgttt
     5041 caatgcggtt tcttttatac aaatatttta cttgtattcc gtacaactgg gagatggatt
     5101 ccatggtttc acccggtttg accaggtgaa aagtggtttt
//
LOCUS       DAOQXT010000189         4694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_18551_length_4694_cov_5.380254, whole genome shotgun sequence.
ACCESSION   DAOQXT010000189 DAOQXT010000000
VERSION     DAOQXT010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18551_length_4694_cov_5.380254"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PKM27_18980"
     CDS             complement(<1..363)
                     /locus_tag="PKM27_18980"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR09413.1"
                     /translation="MKLKISLALILTLTGIPLIAQNPVELGTVKWQRDLQQSVTLSKQ
                     SGKPVFILFQEIPGCLTCRRYGSEVLSHPLLVEGLEQEFIPLAIYNNKGGEDARVLKQ
                     FGEPAWNNPVVRIVDAGLN"
     gene            complement(374..1075)
                     /locus_tag="PKM27_18985"
     CDS             complement(374..1075)
                     /locus_tag="PKM27_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09414.1"
                     /translation="MKFLRFIFLCLPLWSLAQTGTIKGAWQLSGQGEPGETATLIAAD
                     QYLSVAVYKETPPQFVRTYGGSYRLEDGKCILHLEFDTKDTTAIGTDIHYKMTLSGNT
                     LTLENQAKVSWTRVDEGEAPLAGCWRITARAGNDGNMNPMQPGPRKTLKINSATRFQW
                     VAINPATKQFFGTGGGKYSYAEGKYTEFIGFFSRDNSRVGSQLGFDAKVEEGKWYHSG
                     KSSTGNPVSELWEKQ"
     gene            1209..2105
                     /locus_tag="PKM27_18990"
     CDS             1209..2105
                     /locus_tag="PKM27_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09415.1"
                     /translation="MLKPAIYIISGLGLLAACKSPTSREEMVQQALDTPMVKTHKLIQ
                     KWETDTLLKTPESVLYDSKNQVLYVANMDGDAWAKDGKGSISKVGLDGTILKAEWVKG
                     LNAPKGMGLWNGRLFVADLTQIAVINIATGLIEGAINVTGSKGLNDLSIDASGVIYVT
                     DSQGKRLYRVENNSTTLFLDSLKGPNGVLVRGADLYILDAGAMYKVNKNKSLSLITDG
                     LEGGADGIENVKGDEFILSCWAGTIWYLNPNGTKEMVFDGKSLGKNTADIGLDPVSKM
                     VYVPTFFKNSVIAFEIGEISGQ"
     gene            2273..>4694
                     /locus_tag="PKM27_18995"
     CDS             2273..>4694
                     /locus_tag="PKM27_18995"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR09416.1"
                     /translation="MKSFLTFFFWTAGIWWLPAQMISPGADWFIREVRLQNEVIPIEA
                     KPAISFEVNNTYYESGDPKIPIQLRWMTGDSGTGIRFINSGKDTLTLKNVLPLGQNKD
                     LVYITGSGDHALSRTHLYVPGCSPVNVIVPDNAWNLGYASMPLNSRLSIYAFTRRKSQ
                     DKALRRRFETVLYPGGSVYYEYHADTFSGAWQQGLRACFRDRKLYDLATFDDSLYRRP
                     DLQWIRSAKMMHLIMGWDQKLYDRKQGKYVLRDFIHKSKAWYGGDDVIGIWPTWPALG
                     IDQRNQWDLFRDLPGGIKALHKLSEDCQREGVRFFISYNPWDESTRKEDHLKGMADLV
                     YQTDADGVVLDTRGSSSKELQAAADRVRPGVIMYSEGMAVPADMENILAGRVHNALYY
                     PPVLNLNKLIQPEFGIFRVAELFMEPVRREIHTSLFNGYGIEFNIFHPGNPEGLEEQY
                     KYIGRSVRILREHTSLFNGRDWTPLHPSFKDGVLINRWPGTMKEIYTVYNSLPSGYRG
                     HLFEAPEGPGHWLDLWNHKELDPESIEGKVRISLELEPYPQSWQGTNNEGSMGVVARF
                     DDLLTVRLYRGDLLDLSASSGSRILVWAGAPSYSKSPSRLDIRSQTLNLTTLFPGHEG
                     DYVIQLFDSLELIDERIVSIAPATARYIAVTGRSSRHDLGPEMVEIPADTFTFYSSHG
                     DDFIPYPSWSEGGHHAMPSFHMDRHPVTNKEFETFLVKSKYKPRYKSNFLKHWENGKL
                     PKGLENHPVVYVSREDIQAYCAWAGKRLPTEIEWQYAAQTSKRNPWPWGEEAGIQGVK
                     SEIITGTLT"
BASE COUNT         1136 a         1199 c         1247 g         1112 t
ORIGIN      
        1 attcaggcct gcatccacaa tccggaccac gggattgttc caggcgggtt ctccgaattg
       61 tttgagcacc ctggcgtctt cccctccttt gttattgtat atggccaggg ggatgaattc
      121 ctgttcgagg ccttcgacaa ggaggggatg cgacaaaact tcggagccgt atcgccggca
      181 ggtgaggcaa ccgggaattt cctggaaaag gataaaaaca ggtttgcccg actgtttaga
      241 cagggtgact gactgctgga gatcccgttg ccattttaca gtacccaatt ctaccggatt
      301 ttgggcaatc agagggatac cggtcagggt gaggatcaag gccagggaga tcttcaattt
      361 catggataag agcttattgt ttctcccaaa gttcacttac cggattgccg gttgaacttt
      421 tgccgctgtg ataccatttg ccttcttcga ccttggcgtc gaaacccagt tggctgccta
      481 cccggctgtt gtcgcgggag aaaaagccga tgaattccgt gtatttgcct tcggcataag
      541 aatattttcc ccctccggtt ccgaagaatt gtttggtggc agggttgatc gccacccatt
      601 ggaagcgggt cgcgctgttt attttcaggg ttttcctggg gcctggctgc atcgggttca
      661 tgtttccatc attgccggcc ctcgccgtga ttctccagca accggccagc ggggcttctc
      721 cctcatccac ccgggtccag ctgaccttcg cctggttttc cagggtcagg gtatttccgg
      781 aaagggtcat tttataatgg atatctgtgc caatggccgt cgtgtctttg gtgtcaaatt
      841 caaggtgcag gatgcatttc ccgtcttcga ggcggtagct gcccccataa gtgcggacaa
      901 attgtggagg cgtttcttta taaacagcga ccgaaaggta ttggtcagcc gcgatcagag
      961 tggccgtttc accaggttct ccctggccac tcagttgcca tgccccttta atagtgcctg
     1021 tttgggccag ggaccacagg ggcaaacaaa ggaaaatgaa tctcaggaat ttcatgggtt
     1081 caatggtatg ctgaaaatgg gataaaatat tgctaaagtt atgggaaatt atccaacgat
     1141 ttgaaaatgc agcttccggt caatccttgg cttagactct ttgtttaatt tgcggaaaat
     1201 tcaataccat gttaaaacca gcgatatata ttataagcgg cctgggactt ttggcggctt
     1261 gcaaatcccc cacatccagg gaagaaatgg tgcagcaggc cctggatacc ccaatggtca
     1321 aaacccataa acttattcag aaatgggaaa cggataccct tttaaaaacg ccggagtctg
     1381 tgctttacga ttccaaaaac caagtcctgt acgtagccaa tatggatggg gatgcctggg
     1441 ccaaagacgg gaagggttcc atttcgaaag tcgggctcga cggtaccatt ctcaaggcgg
     1501 aatgggtgaa aggcctcaat gcccccaaag gcatgggcct gtggaacggc cgcttgtttg
     1561 tggctgacct gacccagatc gccgtcatca atattgccac cggcctcatc gaaggagcca
     1621 ttaacgtgac gggatccaag ggactgaatg acctttccat tgatgcctca ggagtcatct
     1681 acgtcacaga ctcccagggc aaacggctgt accgggtgga aaacaattcc acaacccttt
     1741 tcctcgattc cctgaaagga cccaatgggg tgctggtccg gggtgcggac ctttatatcc
     1801 tcgatgccgg agcgatgtac aaagtcaata aaaacaagtc cctctcgctg atcaccgacg
     1861 gattggaagg gggtgccgac gggatcgaaa acgtaaaggg cgatgaattt atcctttcct
     1921 gctgggccgg caccatctgg tacctcaatc ccaacggcac aaaggagatg gtctttgacg
     1981 gcaaatccct ggggaagaat actgccgata tcggcctgga tccggtgtcc aaaatggtct
     2041 acgttcctac tttttttaag aactcggtga tcgcttttga gattggtgaa atcagtggtc
     2101 agtaatcagg gatcagggat cagtacggcc cttgacaact tgcacagaga agcgagaagc
     2161 gagaaacgag aagcgagaaa cgagctgcga gaagcgagaa gccagaagcg agatagactt
     2221 gctaacttgc taccgattac tgactactga ctactgactc ctgattattg atatgaaatc
     2281 ctttttgact ttttttttct ggactgcagg catttggtgg ttgcctgccc aaatgatcag
     2341 ccctggcgca gactggttta tccgggaagt caggctgcag aatgaggtga ttccaataga
     2401 ggccaaaccc gcgatcagtt ttgaagtgaa caacacctat tacgaatccg gcgatccgaa
     2461 aattccaata caactgagat ggatgaccgg agattccggg acggggattc ggtttatcaa
     2521 ttcaggaaaa gatactctga ccctaaaaaa tgtacttccc ctgggccaga acaaagacct
     2581 ggtttatatc acggggtcgg gggaccatgc gctttcaagg acccacctct atgtacccgg
     2641 ctgctctccg gtcaatgtga tcgtcccgga taatgcctgg aacctgggat atgcctccat
     2701 gcccctgaac agccggttaa gcatctatgc tttcacccgc aggaaatcgc aggacaaggc
     2761 cttgcggaga cgatttgaaa ccgtgcttta ccccggtggt tcggtgtatt acgaatatca
     2821 tgcggacact ttttccggtg cctggcaaca ggggctcagg gcttgtttcc gggaccggaa
     2881 actgtatgac cttgcaacgt ttgacgattc cctctatcgg cgcccggacc ttcaatggat
     2941 ccggtctgcg aaaatgatgc acctcatcat gggctgggat cagaaactgt atgaccgaaa
     3001 gcaaggcaag tatgtgctcc gggactttat ccacaaaagc aaggcctggt acggcggaga
     3061 tgacgtgatc ggcatctggc ctacctggcc ggccctgggt attgaccaac gcaatcaatg
     3121 ggaccttttc cgggacctgc ccggcgggat caaagccttg cacaaacttt cagaggactg
     3181 tcagcgggaa ggagtgcgct tttttataag ctataatccc tgggacgaaa gcacccggaa
     3241 ggaagaccac ctcaagggca tggccgacct ggtatatcaa accgatgccg acggagtggt
     3301 gctggacacc agggggagca gcagcaaaga actccaggct gccgcggacc gggtgaggcc
     3361 cggagtgatc atgtacagcg aaggcatggc ggtgcccgcc gatatggaaa acatccttgc
     3421 cgggcgggtg cacaatgctt tgtattaccc cccggtttta aaccttaata aactgatcca
     3481 gccggaattc ggaatattcc gggtggccga attattcatg gagccagtgc gccgggagat
     3541 ccatacttcc ctgtttaatg ggtatgggat tgaattcaac atctttcatc cgggcaatcc
     3601 cgaaggcctg gaggaacaat ataaatatat aggccgttcg gtgcgcatcc tccgcgagca
     3661 cacaagcctg ttcaacggcc gggactggac cccactccat ccttccttca aagatggggt
     3721 cctcatcaac cgttggccgg ggaccatgaa agaaatctat accgtttaca acagtttgcc
     3781 ttccggctac cggggccatc tctttgaagc acccgaaggc ccgggccatt ggcttgatct
     3841 ttggaatcat aaggaactgg accctgagtc catcgaggga aaagtccgga tttcccttga
     3901 actggagccc tatccccaat cctggcaggg caccaacaat gaagggtcca tgggggtggt
     3961 cgcccggttt gacgatttgc tgacggtcag gttgtaccgc ggagacctgc ttgatctatc
     4021 ggcctcctcc ggttccagga tcctggtttg ggcaggtgcg ccatcctatt ccaaaagccc
     4081 ttcaagactg gatatccggt cacagaccct caacctgacc actttgtttc cggggcatga
     4141 aggggattat gtgattcaac tattcgattc cctggaactg atcgatgaaa ggattgtgag
     4201 catcgctccc gctacggccc gttatattgc ggtgaccggc cgatccagcc gccatgacct
     4261 cggtccggaa atggtggaaa tccctgcgga tacctttacc ttttattcca gccacggcga
     4321 tgacttcatt ccttatccat cctggtccga aggcggtcat catgccatgc cttctttcca
     4381 tatggatcgt catcccgtca ccaataagga gtttgagacc ttcctggtta aatcaaaata
     4441 caaaccccgg tacaagagta attttttgaa acattgggaa aacgggaaat tgcccaaggg
     4501 cctggaaaac catccggtgg tttacgtatc ccgggaagat atccaggctt attgtgcctg
     4561 ggcgggcaag cgccttccca cagagatcga atggcaatac gcagcccaga cttctaaaag
     4621 aaatccatgg ccctggggtg aagaggccgg cattcaaggc gtgaaatcgg agatcatcac
     4681 cgggaccctt accc
//
LOCUS       DAOQXT010000190         4643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_18850_length_4643_cov_4.784220, whole genome shotgun sequence.
ACCESSION   DAOQXT010000190 DAOQXT010000000
VERSION     DAOQXT010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18850_length_4643_cov_4.784220"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="PKM27_19000"
     CDS             <1..2134
                     /locus_tag="PKM27_19000"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNR09417.1"
                     /translation="DAGIFSARGIGQTTREAIKIRQINAPLESSGQSLNTAFKTLEAE
                     ARAELRRFRVLNENIRIGRRMIYLRFKGQDQVIAINWVKGMDILSEFRAEYRRIYQST
                     LAFEVEIEKILVRVIEKSGDDEIKNIRINRKKSPPASGQKKFIRWAELEPGCRLTGPC
                     TVSSNQASVLVAEGWKLQVQDNKDLVLIPERNRHEVQNWAPAINLELFQNRFRSIAEQ
                     MGAQLQYSAFSVNVKERLDFSCAILDDQARLLVNAPHIPVHLGSLGISARLVLRDFEL
                     GPGDILLSNHPSYGGSHLPDLTLLKAVYDDHQRLIGYVTNRAHHAEIGGMTPGSMPAF
                     ATRLDEEGVIFKPTYIYRGGRADWKQVRKCLTSARYPSRNPDLNILDLRAAIAALLAG
                     EKRLQSLAKTYGREYLRKQMRRILYQGKEQIGEYIRKHEGARLRAMEYLDDGHCIRLV
                     LSIKKGKMTLDFSGSSGVHPGNLNANPAIVQSVVLYTLRLLCDRDISLNEGILHQVKI
                     ILPQGFINPLFPASDADSPAVVGGNTEVSQRLTDCLIKAFGLAACSQGTMNNFLFGNK
                     KYTYYETIGGGTGAGPGFHGRSAIHQHMTNTKITDPEELEWRYPVRLEKFEIRRGSGG
                     SGRWQGGNGICRQIRFLEPMTVTILAQHRKEKPYGLEGGFPGKCGKEMLFSKNQASVL
                     NSNQSFEVKSGDMVKIETPGGGGWGKLK"
     gene            complement(2165..3397)
                     /locus_tag="PKM27_19005"
     CDS             complement(2165..3397)
                     /locus_tag="PKM27_19005"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNR09418.1"
                     /translation="MVDGEVISIPNLSASGRYVRIWVDNTGYANNYLHLAEVRVLGCC
                     TSTDANALVLSSNASEYIQGSSFAVDATMEGDPSQFSRVEFYNNGSLLSTDLSFPFSH
                     LVSPASAGSYLFHAKAIGTCSEINSNFLNITTTKNCNDGYKNGVETGIDCGGDCTPCS
                     QTCLSTVNLAQGMTASQSSNFNSSNSYPASRAVDGSIASASFNHTNAELQPWWEVDLG
                     ASKWVTDLEIVHRTGCSACAGRVKKFKIFLTETRVSGFGASEGMIYLYDNPVGLANGA
                     VITIPGLSASGRYLRIWADNGNAANYLHFAEVKVMGCTPIAALVNEPAIPDPQKNNDL
                     ARIRIYPNPVDDFINLQFDFSPAPGTLFQIIDINGKLLQTGLLSDPKIDFRPFPAGSY
                     FLRILTREKTYPFKIIKL"
     gene            complement(3379..>4643)
                     /locus_tag="PKM27_19010"
     CDS             complement(3379..>4643)
                     /locus_tag="PKM27_19010"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNR09419.1"
                     /translation="STVSSGLSIGILPSCSDGIKNGDEEGVDCGGTYCDPCLPACNAS
                     FNLSLGKQVSQSSNFNASNSYPPSFVNDGNLSNFNHTGLELQPWCQIDLGASYQVSEI
                     EIVNRKDCCGDRLKRFRVFVSEEPVQSFQSNDYIFEFNNSKVLSNGEVINIQNINKAG
                     RYVKVWMDNTNFVNNYLHLTEIKVMGCCLNSNTPTVDFEPAPSTYIQGENITVTAVAE
                     DLGGTVSKVEFYNGMVLLGTDFTPPYSHTIIKAMSDMYQLTAKAYDNCDGATVTEPWI
                     IKTSAECDDGFQNGAETGVDCGGVCKPCLLTCTSPVNISQGKTASQSSNFNSSNSYPA
                     SQSIDGNIASSSFNHTGAEVQPWWQVDLGQVFSVSTIEIHNRKGCSACSGRIINSGFL
                     SVMQRLVVIPQKVMYFNTTIRLEWWMAK"
BASE COUNT         1263 a         1119 c         1141 g         1120 t
ORIGIN      
        1 ggatgccggc attttcagcg cccgtggcat tgggcagacc acccgggagg ccatcaaaat
       61 caggcaaatc aatgccccgc ttgaatcctc cggccaatcg cttaataccg cctttaaaac
      121 actggaagcg gaagccaggg ccgaattgcg ccgatttcgt gtactgaatg aaaatatacg
      181 aataggaaga aggatgatct atctccgttt caaaggccag gaccaggtca ttgccataaa
      241 ctgggtgaaa gggatggata tcctttctga attcagagcc gaataccgcc gtatatacca
      301 gtccaccttg gctttcgagg tggagattga aaaaatcctg gtccgggtga tcgaaaaatc
      361 cggggatgac gaaatcaaga atattcggat caacaggaaa aaaagtcctc ccgcatctgg
      421 tcaaaaaaaa ttcatccggt gggctgaact ggagccggga tgccggctca ccgggccttg
      481 tacggtatcc agcaaccagg ccagcgtact ggtagcggaa ggttggaaac tacaggtcca
      541 ggacaacaag gacctggtgt tgatacctga acgtaatcgc cacgaggttc aaaactgggc
      601 cccggccatc aacctggaat tgttccaaaa ccggttccgg tccattgccg aacaaatggg
      661 ggcccaactg caatacagtg cgttttcagt gaatgtcaaa gaaaggctgg acttttcctg
      721 cgccatcctc gatgaccagg caaggctgct ggtaaatgcc ccgcatatcc ccgtgcacct
      781 gggcagcctg ggtatcagtg caagactggt cctccgggat tttgaacttg gccccgggga
      841 tatcctgctg agcaatcatc ccagctacgg gggatctcat ctgcctgatc tcaccctgct
      901 caaagcggtt tacgatgacc atcaacgatt gattggatat gtcaccaacc gggcccacca
      961 tgcggagatc ggtggaatga ccccgggatc gatgcccgcc tttgcaacac ggctggatga
     1021 agaaggggtc atttttaagc ccacttatat ctatcgcgga ggcagggcag actggaagca
     1081 ggtgagaaaa tgcctgactt cggcaagata tccttccagg aacccggacc tgaatatcct
     1141 ggacctccgt gctgcgatag ccgccctcct ggcgggagaa aaaaggcttc aatcccttgc
     1201 caaaacctat ggcagggaat acctccgcaa acaaatgcgc agaatattat accagggtaa
     1261 ggaacagatc ggggaatata tccgcaaaca cgaaggcgcc cggttaaggg ccatggaata
     1321 cctggatgac gggcactgca tccggctggt cttgtcgatt aaaaagggta aaatgaccct
     1381 ggacttttca ggcagttcgg gagtccatcc gggcaatctc aatgccaatc ccgccattgt
     1441 tcagagcgtg gtcctttaca cgcttcgttt attgtgtgac cgggatatca gcctgaatga
     1501 aggcatattg catcaggtga aaatcatcct cccgcagggg ttcatcaatc ccctgtttcc
     1561 cgcttcggat gcagacagcc ctgcggtggt aggcggaaac acagaggtca gccagcgcct
     1621 tacagattgc ctgatcaaag cctttggact ggcggcctgc agccagggca ccatgaacaa
     1681 ttttttgttt ggaaataaaa agtacaccta ttatgaaaca attggaggcg gaaccggagc
     1741 cggccccggt tttcacggca ggtccgccat ccatcagcat atgaccaata caaaaatcac
     1801 ggacccggaa gaactggagt ggcgctaccc tgtccggctg gaaaaatttg agatcaggag
     1861 ggggtcggga ggctccggcc gatggcaggg gggaaatggc atttgccggc agatccgttt
     1921 cctggagccc atgacggtca ccatcctcgc gcaacaccgg aaagaaaagc cttatggctt
     1981 ggagggagga ttccccggaa aatgcggcaa ggaaatgcta tttagcaaaa atcaggcctc
     2041 ggtgctgaat tcaaatcaat cctttgaggt gaagtccggg gacatggtaa agattgagac
     2101 gccgggcgga ggaggatggg gaaagttgaa gtaaatcctg tcaacaagta tatgttattt
     2161 gctattacaa tttgatgatt ttgaatgggt aagttttctc ccgggtcagg atccgcaaaa
     2221 aataagatcc tgcggggaat ggcctgaaat cgatttttgg atcactcaac aaacctgttt
     2281 gaaggagttt tccattgata tcgattattt ggaacagggt tcccggtgca ggtgaaaaat
     2341 caaattgaag gtttataaaa tcgtccaccg gattgggata tatccggatt cgagcaagat
     2401 cgttattttt ttggggatcg ggaattgcag gttcattcac taatgctgca ataggggtac
     2461 agcccatgac ttttacctcc gcaaaatgca agtagtttgc agcatttcca ttatcagccc
     2521 atatccttaa gtagcgtccg gaggcactca ggccaggaat agtaatcaca gcaccgtttg
     2581 ctaagccgac cgggttgtca taaagataaa tcatcccctc tgacgctccg aaaccactga
     2641 ccctggtttc tgtcaaaaat atcttgaatt tcttaactcg acccgcgcaa gctgaacaac
     2701 ctgtccggtg aacaatttcg aggtctgtaa cccatttgga tgctccgagg tcaacttccc
     2761 accaaggttg taattcggcg ttggtatgat taaaacttgc ggaagcaatg gaaccatcta
     2821 cggccctgga agcaggatag gaattagagg aattaaaatt gctcgattga ctggcagtca
     2881 tgccttgggc gagattgacc gttgacaaac aggtttgtga acatggggta cagtccccac
     2941 cacaatctat accggtctcc actccgttct tgtaaccatc gttgcaattt ttggttgtcg
     3001 ttatgttcag gaaatttgag tttatttcac tgcaagtccc aatggccttt gcgtgaaaca
     3061 ggtagctgcc tgctgaagcg ggagaaacca agtggctgaa tgggaaagaa agatcggtgg
     3121 acagcagact tccattgttg taaaattcaa ctcttgaaaa ctgggatggg tcgccttcca
     3181 tggttgcatc cacggcaaaa gaactacctt ggatatactc ggatgcattt gacgaaagca
     3241 cgagggcatt ggcatctgtg gaggtgcaac aacccaaaac cctcacttct gcaaggtgga
     3301 ggtaattgtt tgcatatcct gtattatcca cccaaatcct gacatatcgt ccggaagctg
     3361 ataaattggg gatggaaatc acttcgccat ccaccattcc agccggattg tcgtattgaa
     3421 atacataact ttctgaggaa taactaccaa ccgctgcatt actgataaaa accctgaatt
     3481 tataattcgg cctgaacaag cagaacaccc cttcctgttg tggatctcaa tggtgctgac
     3541 agaaaatacc tgtccaaggt caacctgcca ccaaggttga acctcggcac cggtatgatt
     3601 aaaactggaa ctagctatat tcccatcgat ggactggctg gctggataag aattggatga
     3661 attgaaatta ctggactggc tggcagtttt accctgactt atattgaccg ggctggtaca
     3721 ggtgagaagg caaggtttac aaacaccccc gcaatccaca ccggtctcgg ccccattttg
     3781 aaacccatca tcacattccg ctgaagtctt gataatccaa ggctcagtaa ctgtagctcc
     3841 gtcacaatta tcatacgctt tggcagtcag ttgatacata tcagacatag ctttgatgat
     3901 ggtatggctg tacggtggag tgaagtcagt acccaataat accataccat tatagaactc
     3961 aacctttgaa accgttccac ccaagtcctc ggccaccgca gtcaccgtta tgttttcacc
     4021 ctggatatag gtggacggag ccggctcgaa atcaacggtt ggtgtgttgc tgttgagaca
     4081 acaacccatc accttgatct cagtcaggtg caagtagtta tttacaaaat ttgtattgtc
     4141 catccatacc tttacatatc ggccggcttt gttaatattt tggatattaa tcacttcccc
     4201 gttggacaaa acctttgaat tgttgaactc aaagatataa tcgttactct ggaatgattg
     4261 gaccggttct tctgaaacaa atacccggaa tcttttaagc ctgtcgccac aacaatcttt
     4321 tcggttgaca atttcgattt ccgaaacctg gtagcttgcc cccaaatcaa tctggcacca
     4381 gggttggagt tctagaccgg tgtggttgaa attggaaagg tttccatcat taacaaagga
     4441 aggaggataa gagttagatg cattaaaatt gctggactgg ctgacctgtt ttcctagact
     4501 caggttaaat gaagcattac aagccggcaa acatggatcg caataagttc ctccgcaatc
     4561 caccccctcc tcatccccgt tcttgattcc atcgctacaa cttgggagga ttccaatact
     4621 cagcccggaa gaaactgtac tga
//
LOCUS       DAOQXT010000191         4624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_18959_length_4624_cov_6.171153, whole genome shotgun sequence.
ACCESSION   DAOQXT010000191 DAOQXT010000000
VERSION     DAOQXT010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18959_length_4624_cov_6.171153"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKM27_19015"
     CDS             <1..872
                     /locus_tag="PKM27_19015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR09420.1"
                     /translation="RIWEKFKSRIREKMPDQRLDLLLRIPLIGALIKKRVRNKLGFSK
                     TVLFYSAAAPISESTLVFFDKLGITIRQVYGMTEDSALSHLIPPGENRIGSVGRKMRG
                     VETRISENGEIRVKSQALMKGYYKEPELTALMFDEEGYLKTGDQGEYDPEGYLFLTGR
                     IKDQFKTDKGKYISPAAIEMKLLTHPDIEMACVVGAGIPQPVALLNLTEAAAGKPRAE
                     LARSLSAFLQGVNAGLDNFERLKKLIVTPAWTIGNGLITPTLKVKRNELEKRYAPKYA
                     QWYEERATVIWEE"
     gene            complement(902..1570)
                     /locus_tag="PKM27_19020"
     CDS             complement(902..1570)
                     /locus_tag="PKM27_19020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR09421.1"
                     /translation="MELNQTMTEEEILDHTTAFVQHELRESERGHDWWHVLRVYQNAM
                     KIVESEPGDPFIIRLAALLHDIADAKFFGGDESIGPSKARDFLTKLGVRPSVTDQVIT
                     IIENISYKSNLDSRAIRSPELDIVQDADRLDALGAIGIARAFHYGGYKNREIYNPDLP
                     PETELTGEKYKKSTAPTINHFYEKLLRLKDLMNTPTGKRMAVERHRFMEEFLHRFYKE
                     WEGG"
     gene            complement(1561..1848)
                     /locus_tag="PKM27_19025"
     CDS             complement(1561..1848)
                     /locus_tag="PKM27_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transporter permease"
                     /protein_id="HNR09422.1"
                     /translation="MNTLFWMGWYLVLGATLVAFYFFFAGLADGSVSQSNMGIWMWLV
                     LGLPALLGITWWLKGQDRIKTALGLLSIPSLLVIAYLIFIAAVLFGNNKWN"
     gene            complement(1845..3692)
                     /locus_tag="PKM27_19030"
     CDS             complement(1845..3692)
                     /locus_tag="PKM27_19030"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR09423.1"
                     /translation="MLVFRLCLIVLAFYQTAYNQADPAIRQSYEDALSRVVVLKNEQQ
                     SIPVQDLTRTFTLISSGPDRSEILDKIKLYAPVKTLRPQDLDSMKGPVPGWVLAEIDL
                     ADAGQPGLTAWSEGLERIKGEYLLILRHGKPGSLPQGLFRKAKGILFSWGDSGYLPIQ
                     LVQVLFGAAAATGTLPFTLNAEYVSGSGLRTRSLQRLTYGPPECLGIDGAALHEKLNR
                     AISDAIESKAFPGANLLIAKDGMVLYHEAFGHPTFESSQPLKKDDLYDMASITKVLAA
                     TLASMVLHSEGKFSSLRTVADYLPEFRNSNKADLLWKDILTHRARLPGFIPFYRYTVK
                     GPDKYVPRSLKKTFSRKYNIKINDHLYLHRRYPAYMMKGIKKVPLLEKEGYVYSDLSM
                     ILMYKTVEAISRMPFDQFLNERIYKPVGAYETGFQPSRRFDPARIVPTERDSFWRRAL
                     VHGYVHDENAAMLGGISGHAGLFMHANDMAKLAQLLLNKGSYGGREYIRPETVDLFTS
                     YQYPEIGNRRGLGFDKPMLRFSHNASYVTQEASPESYGHSGFTGTLLWVDPRYQLTYI
                     LLTNRVYPTRANNKIGQWSLRPCIQQIIYDSILNPGPAADRSASTTTCE"
     gene            3691..>4624
                     /locus_tag="PKM27_19035"
     CDS             3691..>4624
                     /locus_tag="PKM27_19035"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNR09424.1"
                     /translation="MKHFFALNLPFLHQLSSVMHKCLLFCLFIGFWIVQTGCSAAYKL
                     KKTSRAITDSVRYNPIFSKHFTGFVLYDPEEGKVLADLNGSRYFTPASNTKILTLYTA
                     LSLLPDPLPSFRYRRSGDSLFVRGMGDPSLLHPLWPDHEGLKLMKTAGSLFLTEDGGM
                     DPALGPGWAWDDFADAYQAEKSPLPVYGNLARFSPAVQGGGYSAFPSYFQSRWVQADT
                     TAMAGQARRGFLRSTFENEFILPPADTFILNIPFRSGAETIKSLLADTLHVPVKSGWS
                     PPGLSWNTAYGVDADSVYARMMKVSDNFIAEQLIL"
BASE COUNT         1086 a         1162 c         1258 g         1118 t
ORIGIN      
        1 cgaggatctg ggaaaagttc aaaagcagga tcagggaaaa aatgccggac caacgtcttg
       61 acctcctgct ccgtattcct ttaatcgggg ccctgatcaa aaaaagggtc cgtaacaaat
      121 tgggtttctc caaaacggtt ttgttttaca gcgctgcagc tccgatatca gagagcaccc
      181 tggtattttt tgacaagctc ggcatcacca taaggcaagt ctatggcatg accgaggatt
      241 ccgccctgtc ccacctgatc cctcccgggg aaaaccgcat cggttccgta ggaagaaaga
      301 tgcggggagt ggaaacccgg atttcggaaa atggagagat ccgggtgaag agccaggccc
      361 tgatgaaagg ttattacaag gagcccgaat tgaccgccct gatgtttgac gaggagggct
      421 atttaaaaac gggagaccag ggcgaatacg atccggaggg ttatcttttt ctgaccggcc
      481 ggattaaaga ccagttcaag acggacaagg ggaaatacat ttctcctgct gccattgaaa
      541 tgaaattatt gacgcatccc gatattgaaa tggcctgtgt ggttggagcc ggcatcccgc
      601 agccggtcgc cctcctcaac ctgactgaag cggctgccgg aaaacccagg gcggaattgg
      661 caaggtccct ctcggccttt ctgcaaggcg tcaacgccgg cctggataat tttgagcgcc
      721 tcaaaaaatt gattgtgacc ccggcctgga ccatcggcaa cggcctgatc actcccaccc
      781 tgaaggtaaa aagaaatgaa ctggaaaaac gttatgctcc gaaatacgcg caatggtatg
      841 aggaaagggc gaccgtaatc tgggaagaat aatcatgaac aaaaagccca tgggttttcc
      901 atcagcctcc ttcccattct ttataaaaac gatgcaggaa ttcttccatg aaccggtgcc
      961 tttcgacggc cattcgtttc ccggtaggag tgttcatcag gtctttcagg cgcagcagct
     1021 tttcataaaa atgattgatc gtgggggctg tgctcttttt atatttttcg ccggtcagtt
     1081 cggtttccgg aggaaggtcc gggttgtaga tttccctgtt tttgtatcct ccgtaatgga
     1141 aggccctggc aatccctatg gctcccaggg catccaggcg gtcggcatcc tgaacgatgt
     1201 ccagttccgg actgcggatg gcccggctat cgaggtttga tttataggag atgttttcta
     1261 tgatggtgat cacctggtcg gtgacggatg gcctcacgcc cagttttgtc agaaaatcgc
     1321 gggctttgga tggcccgatg gattcatctc ctccgaagaa ttttgcatct gcaatatcgt
     1381 ggagaagggc agccaggcgg atgatgaaag gatcgccggg ttccgattcg acgattttca
     1441 ttgcattttg atagacgcgt aagacatgcc accaatcatg tcctcgctcg ctttcccgga
     1501 gttcatgttg tacaaaagcg gtggtatgat ccaggatctc ctcttccgtc atggtttggt
     1561 tcagttccat ttgttgtttc cgaataaaac cgcggcgata aatatcagat aagcgatgac
     1621 caacagggag gggatgctca gaaggcccaa tgccgtttta atcctgtcct gacccttcag
     1681 ccaccaggtg atgcccagaa gggcgggaag gcccagcacc agccacatcc aaatgcccat
     1741 attggattgg gaaaccgaac cgtccgccag cccggcaaaa aagaaataaa aagcgaccag
     1801 ggtggctccg agtaccaggt accaacccat ccagaataag gtattcattc gcatgtggtg
     1861 gtggatgcgg accggtcggc cgccggcccc ggattaagga ttgaatcgta aatgatctgc
     1921 tgaatacagg gcctcaggga ccattgaccg attttgttgt tggcgcgggt gggatagacc
     1981 cggttggtca ggaggatata agtcagttgg tagcggggat cgacccatag cagggtgccg
     2041 gtaaaaccgg aatgaccata actttcaggg cttgcctcct gagtgacata actcgcattg
     2101 tgggaaaacc tgagcatggg tttgtcgaat cccagaccgc ggcggtttcc gatttcggga
     2161 tactgatagg aggtaaacaa gtccacggtt tccggccgga tgtattccct gcccccatag
     2221 ctgcccttat tcaggaggag ctgggcgagt ttggccatat cgttggcgtg cataaacaaa
     2281 ccggcatgcc ccgagatgcc cccgagcatg gccgcatttt catcgtgcac atatccgtgg
     2341 accagggccc gcctccagaa tgaatccctt tcggtgggca ctatgcgggc cgggtcaaac
     2401 cggcgactgg gctgaaatcc ggtttcgtaa gctccaaccg gtttgtagat ccgttcgttc
     2461 aggaactggt caaagggcat acggctgatg gcttccacgg ttttgtacat caggatcatg
     2521 gaaaggtctg aataaacata cccttctttt tccaataagg gaactttctt gattcctttc
     2581 atcatatacg cgggataccg acggtgcagg tacaggtgat cgttgatttt aatattgtat
     2641 ttccggctga aggtcttttt cagggaacgg ggaacatatt tgtccggtcc cttcaccgtg
     2701 tatcggtaaa aagggataaa acccggaagc ctggcccggt gggtgaggat atctttccac
     2761 aacaaatcgg ctttattgct gttgcgaaat tcaggcaaat aatccgccac ggtgcggagg
     2821 gaggaaaatt tgccttcgct gtgcaggacc atggaggcaa gggtggctgc caggaccttg
     2881 gtgatggaag ccatgtcata gagatcatcc ttctttaaag gctggctgct ttcaaaggtc
     2941 ggatggccaa aggcttcatg atataaaacc atcccgtctt ttgcaatgag gagattggcc
     3001 ccgggaaagg ctttactctc aatggcatcc gatatcgccc ggtttaattt ttcatgcaag
     3061 gcggctccgt caatgcccag gcactccggg ggtccgtaag tcagtcgctg caaggaccgg
     3121 gtgcgcaaac cgctgccgga gacatactcg gcattcaggg taaagggcag ggtcccggtt
     3181 gctgcggcag ccccaaataa cacttgcacc aactggatgg gcaaataccc ggagtcaccc
     3241 catgagaaaa gaatgccttt tgctttcctg aaaaggccct ggggcaggga accaggtttg
     3301 ccgtgcctta gaatgagcag atattctccc ttaattcgtt cgagtccttc agaccaggcg
     3361 gtgagccccg gctgccctgc atcggccaga tcgatttcgg caaggaccca accggggacc
     3421 gggcccttca tggagtccag gtcctgtggt cgtagggtct ttaccggagc atataatttt
     3481 atcttatcca aaatctcgct gcgatccgga ccggaactga tcagggtgaa ggtccgggtc
     3541 aggtcctgga ccgggatgga ttgctgctca ttttttaaaa ccaccaccct ggaaagggca
     3601 tcttcataac tttgcctgat cgcgggatcc gcctggttgt aggcggtttg ataaaaggcc
     3661 agcacgatca agcataaccg gaatacaagc atgaagcatt tttttgcgct gaatttacca
     3721 tttttacatc aactgagttc cgtcatgcat aaatgcctcc tgttttgtct gtttatcgga
     3781 ttttggattg tccagacggg atgttccgcc gcttataagt tgaaaaagac cagccgggcc
     3841 atcacggaca gcgtgcggta caatcccatc tttagcaaac attttacagg ttttgtgctc
     3901 tatgatcctg aagaagggaa ggtgcttgca gatctcaatg gctcccggta tttcaccccg
     3961 gcttccaata ccaaaatact caccctgtat accgcgctct ccctgttgcc cgaccctctg
     4021 ccttcgttca gatatcgccg gtcaggggac agcctttttg tgcgcggtat gggtgacccc
     4081 agcctgctcc acccgctgtg gcccgatcat gaggggctga aattaatgaa gactgcagga
     4141 agtttgttcc ttacggaaga tggggggatg gacccggcat tgggaccggg atgggcctgg
     4201 gatgattttg cggatgcata ccaggctgaa aaatccccat tgccggtgta tggcaatctg
     4261 gcccggtttt cacctgccgt ccagggtggg ggatatagcg ctttcccttc ctatttccag
     4321 tcccgatggg tccaggccga taccactgca atggcagggc aagcccggag gggcttcctc
     4381 cggtcaacat ttgaaaatga attcatcctt cctccggcgg ataccttcat cctgaatatt
     4441 cctttcaggt ccggtgccga aaccattaaa agcctgttgg cggatacctt gcatgtcccg
     4501 gtaaaatcgg gctggtcccc gccgggtttg tcctggaaca ccgcttatgg ggtggatgcc
     4561 gattcggtat atgcacggat gatgaaagta agcgacaatt ttattgcgga acaactcatc
     4621 ctgc
//
LOCUS       DAOQXT010000192         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_19271_length_4568_cov_6.341015, whole genome shotgun sequence.
ACCESSION   DAOQXT010000192 DAOQXT010000000
VERSION     DAOQXT010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19271_length_4568_cov_6.341015"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PKM27_19040"
     CDS             complement(<1..569)
                     /locus_tag="PKM27_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09425.1"
                     /translation="MTCLRAFTQGNLVPNPGFEERISCPAHLSDFTGIVKYWFTPTSG
                     TPNFYHSCASQPEVGVPDNYFGHKTAQEGQAYAGIMTSNAYREYISVQLTTPLAAGKM
                     YKLSFYTSIISKSNCNSQGLDALFTPHRPTAEQANDNLNARASLNLTVQYSGDQWIKT
                     EACYRANGGERYLTLGDLTFPQARHNCADG"
     gene            774..935
                     /locus_tag="PKM27_19045"
     CDS             774..935
                     /locus_tag="PKM27_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09426.1"
                     /translation="MKTFKLVLLIVVLSATTWLMAEGVRINLLANDPHNQETVMTQPK
                     AVKTVKALR"
     gene            963..2168
                     /locus_tag="PKM27_19050"
     CDS             963..2168
                     /locus_tag="PKM27_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNR09427.1"
                     /translation="MNKRKAALGFIFVTLLIDVVGLGIIIPVMPSLLSGMTGGTISDA
                     SKYGGWLMFSFAIMQFLFSPVLGALSDQYGRRKVLLISMFGFCLDYLLMAWAPTLSWL
                     FLGRIIAGITGASFTTAAAYIADISTDENRAQNFGMIGAAFGLGFIIGPTLGGILGNI
                     GTRLPFMVSAGLTFINWLYGYFILPESLDEQHRRPFDIRRANPAGSLVQLKKYPTVFG
                     LVGSLVFIYIAAHSVQSTWTYFNMYAFHWNEATVGYSLGFVGLLIALVQAVLIRYTIP
                     TFGPRRSLYLGLLFYTLGLVLFAFATRSWMMFVFSIPYCLGGIAGPALQSTISGQVPR
                     NAQGELQGALTSLMSLTSIIGPLLMTYVFYYFTRKDAWLYFPGSAFLLGAVLILLSTW
                     IAYRKLGKP"
     gene            complement(2213..3277)
                     /gene="selD"
                     /locus_tag="PKM27_19055"
     CDS             complement(2213..3277)
                     /gene="selD"
                     /locus_tag="PKM27_19055"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNR09428.1"
                     /translation="MESVRLTQYSHGAGCGCKISPKNLEVILKSSGTEAPDPRLLVGY
                     DSRDDAAVYDIGHGLATISTTDFFMPIVDDAFDFGRIAATNAISDVYAMGGKPIMAIS
                     VLGWPVNKIPPEIAREVVEGGREVCRNAGIPLAGGHSIDSPEPIFGLAVTGLIQKSKI
                     KRNDQARPGDLLFLTKALGTGILSTAQKKGILEAEDQKMMIDTMCTLNRVGTDLAELE
                     GVHSMTDVTGFGLLGHLLEMARGSGLTAHVDTQRIRLLPNVRNYLEASSHPGGTARNW
                     ESYGHEVGPVDEFWRLVLADPQTSGGLLIAVHPASAAEVADLLNRHGLYCHPVGKMEM
                     ETEGEKRIWIDSKDIFLPSF"
     gene            complement(3249..4304)
                     /gene="mnmH"
                     /locus_tag="PKM27_19060"
     CDS             complement(3249..4304)
                     /gene="mnmH"
                     /locus_tag="PKM27_19060"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNR09429.1"
                     /translation="MLKSLDAEEWLQQESAVIFDVRSPAEFARGHVPGAFSLPLLEDE
                     ERAAVGTAYKQAGKEEAYSLALNLAGPRIKDLVSRARRLAPGPRVNIHCWRGGMRSGS
                     MAWLLHAMGFEEVNVLHRGYKAYRQWVLSRFEPDYPVMVLGGRTGSAKTEVLKQLATR
                     NEKVIDLEKLAHHKGSAFGWIGEAPQPTQEQFENDLAGELNLARESPVLWLEDESEHI
                     GRVRIPRPLFLQVRKAPVFFIDIPKSVRVPHLVHTYARYGDEKLEQSILKIAKRLGGL
                     NTKLALEALKRQDYERVADLALDYYDKTYDTGTGHRDPSTVIRIHTETIDPVHNAGLV
                     LEYKHKPLHGISKTYAV"
BASE COUNT          965 a         1209 c         1226 g         1168 t
ORIGIN      
        1 ccgtccgcgc aattgtggcg ggcctgaggg aaagtcagat cgcccagggt aaggtaccgt
       61 tcacctccat tggcccggta acaggcttcc gtttttatcc actggtcccc ggaatattgc
      121 accgtcaaat tcagggaagc gcgggcattc aggttgtcgt tagcctgttc tgccgtgggc
      181 cggtgggggg taaacagggc gtccagtccc tgggaattgc aattcgattt ggaaatgatg
      241 gaggtgtaaa agctcaattt atacatttta ccggcggcca agggcgtggt caactggacg
      301 gagatgtatt cccggtaggc attgctcgtc atgatcccgg cataggcctg cccttcttgg
      361 gcggttttat gcccaaaata attgtccggg actccgactt ccggctggct ggcacagctg
      421 tgataaaaat tgggagtacc ggaagtgggt gtaaaccaat attttacgat gcctgtgaag
      481 tcgctcaggt gggcagggca gctgatccgc tcttcaaaac ccggattggg gaccagatta
      541 ccctgggtaa aggcccgcaa acaggtcatt gtaagaaaaa agtatgaaag gaccgacttc
      601 aagggtacta aattacacgg ataagggtac ggatcattcc ggagaacgaa aaaccatgga
      661 atccttaaca aaagcacggt tacgtcggat tttttttccc gctcgtcgcc tttacttggg
      721 tcgagccatg cactgcatag atctttgttt cataaaataa taacaaaaga aaaatgaaaa
      781 ctttcaaatt ggtccttttg atcgtggtcc tgtccgccac cacctggctg atggcagaag
      841 gggtcagaat caatcttctg gccaatgatc cccataacca ggaaacggtg atgacccagc
      901 ccaaagcggt caaaaccgta aaagccctca ggtaaatttg ggctatttta gcgctgccac
      961 agatgaataa acggaaggca gcgctcgggt tcatttttgt tactcttctg atagatgtgg
     1021 ttggcttggg gatcatcatt ccggtgatgc cttccctcct ttccggcatg actggaggaa
     1081 ccatttccga tgccagcaaa tacgggggct ggctgatgtt ttcctttgca atcatgcagt
     1141 tcctgttttc accggtattg ggagccttaa gcgaccagta tgggcggagg aaagtgctgt
     1201 tgatttccat gtttggattt tgcctggatt acctcctcat ggcctgggcc ccgacccttt
     1261 cctggttgtt cctgggacgg atcattgcgg gcatcaccgg ggcaagcttt actacggccg
     1321 ccgcttacat cgccgatatc agcaccgatg aaaaccgggc gcagaatttt ggcatgatcg
     1381 gcgccgcctt tgggctggga tttattatcg gacctaccct gggaggtatt ctgggaaata
     1441 tcgggacccg gcttcccttc atggtctccg cgggcctgac ttttatcaat tggttatatg
     1501 gttatttcat cctcccggaa tcgctggacg aacaacaccg gcgcccattc gatatccgga
     1561 gggccaatcc ggccgggtcc ctggtccagc ttaagaagta tcccacggta tttgggctgg
     1621 tgggttccct ggtgtttatt tatatcgcgg cccattccgt ccagagcacc tggacctatt
     1681 tcaatatgta tgcctttcac tggaatgagg ccacggtggg ttattccctt ggatttgttg
     1741 gcctcctgat cgccttggtc caggcggtcc tgattcgcta taccattcca acattcggcc
     1801 ccaggcggag cctttatctc ggattgctgt tttataccct gggccttgtt ttgtttgcct
     1861 ttgccaccag gagctggatg atgtttgtgt tcagtatccc ctattgtctc ggcggtattg
     1921 cagggccggc tttgcagtcg accatatcgg gtcaggtacc ccggaatgct cagggcgaat
     1981 tgcagggcgc cctcaccagc ctgatgagcc tgacgtccat catcggtccg ctcctgatga
     2041 cttatgtttt ttattatttt acgaggaaag atgcctggct gtacttcccg ggttcggctt
     2101 ttttacttgg tgctgttttg attttattga gcacctggat cgcctatagg aaattgggga
     2161 agccatgaac agttcgttag tcagcaagtc agcaagttgg caagttggca agttagaagg
     2221 agggaaggaa gatatccttc gagtcaatcc aaatacgttt ttcaccttcc gtttccattt
     2281 ccattttacc cacaggatgg caatatagcc cgtgccgatt aaggaggtcc gccacttccg
     2341 cggcggaagc cggatgcacg gcaatcaaca gtccgccgct ggtctgcgga tcggccagca
     2401 ccagtcgcca gaattcatcc accgggccga cctcatggcc ataactctcc cagttccggg
     2461 cagttccccc cgggtgactg gatgcttcaa ggtagtttct tacattgggc agcagccgga
     2521 tcctttgtgt atccacatgc gccgtcaggc cgcttcccct cgccatttcc agcagatggc
     2581 ccaacaagcc gaaaccggtg acatcagtca tggaatgcac cccttccagt tccgccaaat
     2641 ccgtgcctac ccggttgagc gtgcacatgg tatcgatcat cattttttga tcttcggcct
     2701 cgaggatccc ttttttctgt gccgtgctga ggatgccggt gcccagggct ttggtcagga
     2761 agagaaggtc gccgggacgg gcctggtcgt ttcgttttat ttttgatttt tggatcagcc
     2821 cggttacggc aagcccgaat atgggttcgg gactgtcaat gctgtgccct ccggccaggg
     2881 ggatccctgc attccggcac acttcgcggc ctccttccac cacttcgcgg gctatctccg
     2941 gcgggatctt attcaccggc cagccgagca cggaaatggc catgatcggt ttaccaccca
     3001 tggcatagac atcactgatg gcattggtgg cggcgatcct gccgaagtca aacgcgtcat
     3061 ccacgatggg cataaaaaaa tccgtcgtac tgatggtcgc cagcccatgc ccgatatcgt
     3121 aaacggcggc atcgtcccgg gagtcgtatc ctaccaggag cctgggatcg ggggcctcgg
     3181 tacccgatga ttttaatatg acttcgaggt ttttgggaga gattttgcat ccgcaacctg
     3241 cgccgtgact atactgcgta agtcttactg attccatgaa gaggtttgtg tttgtattcc
     3301 agcaccaggc cggcattgtg aaccgggtcg atcgtttccg tatgaatcct tatgacggtg
     3361 gaggggtccc ggtgaccagt accggtatca taggttttgt cgtaatagtc cagggccagg
     3421 tccgcgaccc gctcgtaatc ctgccgcttt aaagcttcca gggcaagttt agtattcagg
     3481 ccacccagcc ttttggcgat ttttaaaata ctttgttcca gtttttcatc gccataccgc
     3541 gcgtaggtat ggacgagatg gggcacccgg accgatttgg gaatatcaat aaaaaaaacc
     3601 ggggccttcc gcacttgtaa aaacagaggc cggggtatcc tgaccctgcc gatgtgttcg
     3661 ctttcatctt ccagccatag cacagggctc tcccgggcca ggttcagctc tccggccagg
     3721 tcattttcga attgttcctg ggtaggctgg ggcgcctccc cgatccagcc aaaagcagag
     3781 cctttgtgat gggccagttt ttcgaggtca atgacttttt cattccgggt ggccagttgt
     3841 tttaaaactt ccgtcttggc gctgccggtc ctccctccca gcaccatgac cggatagtcc
     3901 ggttcaaacc gggaaaggac ccattgccgg taagctttat atcctctgtg cagtacgttt
     3961 acttcttcaa aacccatggc atggagcagc caggccatgc ttccgctgcg catgccccct
     4021 ctccagcaat ggatgtttac cctgggcccc ggagcaagcc gccttgccct ggataccagg
     4081 tctttgatcc ggggtccggc caggttaagg gccagggaat aagcttcttc tttcccggct
     4141 tgtttataag cggtgcctac ggccgccctt tcctcatctt ccaacagggg aagggagaag
     4201 gctccgggga catgtccgcg agcaaattca gcaggggagc ggacgtcgaa aatgaccgcg
     4261 gactcctgct gcagccattc ttccgcatcc agtgatttga gcattggact gcaaagataa
     4321 agaagtgttc agggtgaagg ggtgtgaggt ttcccactag gacactatgg gttgcccaca
     4381 acgacacagc gaagcgaagg gcacccatgt ttggacgaag cttagcttcg gcccccccat
     4441 tcggacttcg actcagaaaa catagaatga tattgattcg gaaaaaccgc gatccaacat
     4501 tgtgccttta ggcacttcat acgggtagca tttaagagca ggatatttcg tcccatccgg
     4561 gacgatat
//
LOCUS       DAOQXT010000193         4411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_20230_length_4411_cov_5.625574, whole genome shotgun sequence.
ACCESSION   DAOQXT010000193 DAOQXT010000000
VERSION     DAOQXT010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20230_length_4411_cov_5.625574"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKM27_19065"
     CDS             complement(<1..670)
                     /locus_tag="PKM27_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR09430.1"
                     /translation="MKKKLIALFFLNFLFLTWSCTDLDEEILDETNASGLTDKQAADG
                     ILAPVYARLPDVFLHTAYFAIQEITTDEAILPYRGGTDWGDNGIYIELHKHTYTSTNA
                     NVRNTWNVVLQGISRAITAINSLPSLNDPSVNTYIAEARGMRAYYSMLTLDLFGLVFV
                     KDDLGATSKILRGTEAFNYVRDEFLAVEPQLDPSVGPGRLSKGAVWGLLARLYLNGAV
                     YRAPY"
     gene            complement(689..3724)
                     /locus_tag="PKM27_19070"
     CDS             complement(689..3724)
                     /locus_tag="PKM27_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR09431.1"
                     /translation="MMKILFLNLHFIKYIKNSLIRGICLLWIFMPGRAWAHAALPSET
                     DVLAWVVKGRVTDANTSEPLIGCTVQIKGSGQGVVTDFNGDYSIDVQDQNAVLVFNYI
                     GYQEIEEPVNGRTEINITMSQSVSELSQVVVIGYGSATKKDMTGAVKSLKAAEFNKGI
                     INSPEQLLQGKVAGVNVTSASGEPGSIQSITIRGPGGVRSGSTPLFVVDGVALDNSST
                     GGATNPLNFLNPQDIESIDVLKDASATAIYGARGANGVVLITTKRGKAGTSILNYSGS
                     AGLSTMANPLEVFSADQYRIEVPKVGGVLEDLGADTDWQKEISRRALTHNHNLSLSGG
                     ADKLTYYGSLGLQSQEGILRKSQLNRYTGRININQKFLDDRLDLDLNLNATNTFNYRP
                     PIGALIGGAISANPTYPAYDAQGNPYRYAAGTNPLITLNLEKDVTTINRVLGNISPSL
                     RITKNLRYKLNFGIDNSTSARDIQSLANAVPQQDGRLETVNIYNRNKLIENYFTYTLT
                     KTDYNLSAVVGHSYQKIFLQGRATSINKFPISNIEPQYNPGLGQELTLANNKPSGYAI
                     VNELQSYFSRVNFQFLDKYLVTATVRADGSSKFGENNKYGVFPSFSVGWRLNEEPFFE
                     SLPFSEFKLRAGWGQTGNQEIPSKITQPLFTSQVSASTSYPLANTGPYPAGTSYSRLA
                     NPDIQWEVSTQTDIGLDFGLFNGALAGAIDYFNKKSGKILLEVIPADPVQPAGTVWTN
                     VADMTVTNSGLELDLQYRKTLTRGLSYYLGGNITFIQNNVENSPYTVIPSGSASGSGL
                     TSATINGYVNGNPIGTFFLKEYIGLDDKGLSRFRDVDQDGIITDKDRVALGSALPKQM
                     FNLNGGFSWKAFDFSANLNGVSGNKVYDNTANSNFYKLRLSKGINTTAEAIAEPKESI
                     NNSAPVSSRYLKDGSFVRLNNLSLGYQINTEKLGIRKWVSALRVSLTGENLLLWTKYD
                     GYDPEVNTDRNINGVNSYGIDYLSYPKAKSFIFSLNLTL"
     gene            complement(4028..>4411)
                     /locus_tag="PKM27_19075"
     CDS             complement(4028..>4411)
                     /locus_tag="PKM27_19075"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR09432.1"
                     /translation="EEAGIQGVKSEIITGTLTHTTFTGFDSTLANPGNGKLDPVGSYP
                     KGENPFGLTDLVGSVWQMTADHYRSGSYEYLILKGGSYYKPTASWWYVQGGPQALAWK
                     QMLLLVDQGFERKGTVGFRCVRDKE"
BASE COUNT          989 a         1077 c         1215 g         1130 t
ORIGIN      
        1 aatagggggc ccggtaaacg gccccgttca ggtagagcct tgccagcaat ccccagactg
       61 ctcctttact gagacggccg ggcccaacgg agggatccag ttggggctcc accgccagaa
      121 attcatctct gacgtaattg aaggcttcgg taccccgaag gattttggaa gtggcgccca
      181 ggtcatcttt cacaaacacc aggccgaata aatcgagggt cagcatggaa taatatgccc
      241 gcatccccct ggcttcggcg atataggtgt tcaccgaagg atcattgagc gatggaaggg
      301 aattgatcgc ggtgatcgcc cgggaaatgc cctgcagaac tacattccaa gtattccgga
      361 cattggcatt tgtactggta taggtatgct tgtgcaattc aatgtagatc ccgttatccc
      421 cccagtccgt accgccccgg tagggcagaa tggcttcgtc cgtggtgatt tcctggatgg
      481 caaaataggc agtatgcaag aatacgtccg gcaagcgggc gtagacggga gccaaaatgc
      541 cgtcagcggc ttgtttatcg gtcaggccgg aggcgttggt ctcatccagg atctcttcat
      601 ccaggtcggt gcaactccag gtgaggaaaa gaaaattgag aaaaaacaag gctataagtt
      661 tctttttcat gacgggtctt gttttttatt ataaggttag atttaaactg aaaataaatg
      721 atttggcttt gggataggac aggtagtcga ttccataaga attgactccg ttgatattcc
      781 ggtccgtatt gacttcggga tcgtatccgt cgtacttggt ccaaagcagg aggttttcgc
      841 cggtaaggga gaccctcagc gccgataccc atttccggat cccaagtttt tcggtattga
      901 tctgatatcc gagggacaag ttgttgagcc tgacaaaaga tccgtctttg agatacctcg
      961 aggatacggg agcggagtta ttgatggact ccttgggctc cgcgatggct tctgccgtgg
     1021 tattgatccc tttcgacaag cggagtttgt aaaaattgga attggcggta ttgtcatata
     1081 ctttatttcc ggacaccccg ttgaggttgg ctgaaaagtc aaaggctttc cagctgaatc
     1141 caccgttgag gttgaacatt tgtttgggca gggcgctgcc cagggccacc cggtccttgt
     1201 ccgtaatgat cccgtcctgg tccacgtcgc ggaatcggct caggcccttg tcatccagcc
     1261 cgatgtattc tttgaggaaa aaggtgccga taggatttcc gttgacatat ccgttgatcg
     1321 tagccgaggt caggcccgaa ccggatgccg aaccggaagg aatcacggta tagggtgaat
     1381 tttcaacatt gttttggata aaagtgatat tgcctccgag gtaataggac aatccgcgcg
     1441 tgagggtttt ccggtattgg aggtccagtt caaggccgct gttggtcacg gtcatatcgg
     1501 ccacgttggt ccataccgtg cccgcaggtt gcacggggtc tgccgggatg acttcgagca
     1561 ggatcttgcc tgattttttg ttgaaataat caatggcgcc ggctaaagcc ccgttgaaca
     1621 aaccgaaatc aagtccgata tcggtttggg tggatacctc ccactggatg tcgggattgg
     1681 ccaggcggga ataggaggta cccgcaggat atgggccggt attggccaag ggataggacg
     1741 ttgaggcgga cacctgggag gtgaacaaag gctgagtgat tttggacggg atttcctggt
     1801 tgccggtctg gccccatccg gctctcagtt taaattcact gaaaggcagg gactcgaaga
     1861 acggctcttc attcaatcgc catccgacgg aaaaggatgg gaagacccca tatttattgt
     1921 tttccccaaa tttcgaggaa ccgtccgccc tcaccgttgc cgtgaccagg tacttgtcca
     1981 gaaactggaa gttgacgcgt gaaaaataag actgcaattc gttgacgatg gcatagccgg
     2041 aaggtttgtt attggccagg gtcagttcct ggcccaggcc gggattgtat tgaggttcaa
     2101 tattggagat gggaaatttg ttgatgctgg tagccctccc ctgtaaaaag atcttctggt
     2161 aggaatggcc cactacggcc gacaggttat aatcggtttt ggtcagggta taggtaaaat
     2221 aattttcgat caatttgttc cggttataga tgttgacggt ttccagacgc ccgtcctgtt
     2281 ggggtaccgc gttggcgagg gactggatat cgcgcgcgga ggtggaattg tcgatgccga
     2341 aattcaattt gtaccggaga tttttggtga tgcgaaggga gggggaaata tttcccagaa
     2401 cgcggttgat cgtggtcacg tccttttcca ggttgagggt gatcagcgga ttggtcccgg
     2461 cagcataccg gtagggattg ccttgggcat cgtaagccgg ataagtggga ttggcggaaa
     2521 tggctcctcc gataagcgct ccgatcgggg gacggtaatt aaacgtgttg gttgcgttga
     2581 ggttgaggtc gaggtcgagg cggtcgtcca ggaatttctg attgatattg atccggccgg
     2641 tgtagcggtt tagctggctt tttctcagga tgccttcctg ggactgaagg cccagggagc
     2701 cgtaataagt cagtttatcc gctcctccgg ataaagaaag gttatggttg tgggtgagcg
     2761 ctctgcggct tatttctttt tgccagtcgg tatcggcacc caggtcttcc aatactcctc
     2821 cgactttagg cacttcaata cggtattggt cggcggaaaa gacctcaagg ggattggcca
     2881 tggtagagag gccggcggaa cccgaatagt tgaggatgga agtccccgct ttgccccttt
     2941 tggtggtgat cagcactacg ccattggcac cgcgggctcc ataaatcgcg gtcgccgagg
     3001 cgtccttgag cacatcaatg gattcgatgt cctggggatt gagaaaattg agcggattgg
     3061 tcgctcctcc ggtggaggaa ttgtccaagg ctaccccgtc gactacgaac aaaggcgtgc
     3121 tcccggaacg aaccccgcca ggcccccgga tggtgatgct ttgtatgctg ccgggctccc
     3181 cgctggccga ggtgacattt acacctgcca ccttgccctg aagcaattgc tccggtgaat
     3241 tgatgatccc tttattaaat tcagcggcct ttaaagactt taccgcgccg gtcatgtctt
     3301 ttttcgtcgc gctcccatac ccaatgacca cgacctggga caattccgaa acggattggg
     3361 acatggtgat attaatctcc gtcctcccat tcaccggctc ctctatttcc tgatacccga
     3421 tatagttgaa taccagcacg gcattttggt cctggacatc aatgctgtaa tccccgttaa
     3481 aatcagtgac tacgccctgg ccgcttcctt tgatctgcac ggtacaaccg atcaggggtt
     3541 ccgaggtatt cgcatcggtc accctccctt tgaccaccca cgccaggaca tctgtttcag
     3601 aaggcagggc agcatgggcc caggcccttc cgggcataaa aatccataat aaacagatgc
     3661 cccggatcag gctgtttttg atatacttaa taaaatgcag attcaggaag aggatcttca
     3721 tcatgattga aattgagtta ggttaaatga ctacaggggc taaagtgaaa caatttttgg
     3781 tttctggcat ccgggccgga ttatatattt acgaaaccga tttcgttttg cggaaccgga
     3841 ttgcttccgt ccaccgggct tccgaaatgt tttaatccca accgatttgc tcttgctgct
     3901 gcgaaactac ggttgccagg ttaagtgtaa atgaagccaa ggttagcccg cgattaatgt
     3961 tcctaccatt gtaatttttc acccttcacc cttcgccctt cgcccttcac cctctacctt
     4021 tcctccttta ctccttgtcc ctcacacacc tgaacccaac cgtccccttc ctttcaaatc
     4081 cctggtcgac caacaacagc atttgtttcc aggcaagggc ctgtgggcca ccctgtacat
     4141 accaccagga agcggtgggt ttgtaataac tgccgccttt gagtatgagg tattcgtagg
     4201 aaccgctgcg gtaatggtcg gcggtcatct gccagactga acccacgagg tcggtgagcc
     4261 caaagggatt ctctcccttg ggataagagc ctaccggatc aagctttcca ttgcccggat
     4321 tggccagcgt ggagtcgaaa ccggtgaaag tggtatgggt aagggtcccg gtgatgatct
     4381 ccgatttcac gccttgaatg ccggcctctt c
//
LOCUS       DAOQXT010000194         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_22054_length_4131_cov_5.309127, whole genome shotgun sequence.
ACCESSION   DAOQXT010000194 DAOQXT010000000
VERSION     DAOQXT010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22054_length_4131_cov_5.309127"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..853
                     /locus_tag="PKM27_19080"
     CDS             230..853
                     /locus_tag="PKM27_19080"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNR09433.1"
                     /translation="MPQILHHDDATYHLGVWKIEEPESFFVDRLHEDQRQLPFLPDQR
                     RLQSLAARLLLNRMMESPQVILKNESNKPFLRDDDRYISISHSFEVATVLVARRLCGV
                     DIEKNLDRIERIAAKFLFPGEMDRLQGSQYRRHLYRIWGAKEAMFKAYGLGGIDFKKD
                     LWIDLDEILNNNEVFSSRLLNHEREIRFEMRWLELLPDYILVYGSEI"
     gene            837..1298
                     /locus_tag="PKM27_19085"
     CDS             837..1298
                     /locus_tag="PKM27_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09434.1"
                     /translation="MAPKFDGLMALGFIFLLFACKSSPRQQENFPLPRIHAFDSFYTS
                     FHSDSAYQMRHILFPLEGLPEAMDTARYGAVYYWDKETWRLHRPFDPADTTFVRQFQI
                     IDSTLIREVIAHLMSGYRLERRYSFTGKDWQLIYYAPLRQPVRIEVEIPDR"
     gene            complement(1373..2116)
                     /locus_tag="PKM27_19090"
     CDS             complement(1373..2116)
                     /locus_tag="PKM27_19090"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNR09435.1"
                     /translation="MRWSEKYGLMRSWLMYYAKPFNRRRLTRFYRQFLQAGDLAFDIG
                     AHLGNRSRAFLDLGAWVVAVEPQPLCVQNLVERFGHQDRFELLPYAIGREEGTIDFRI
                     SRRSPTLSTARSTDWQNQINHYSRIKATWDETIQVRQHTLDQLIEWHGLPRFCKIDAE
                     GFEWEIIQGLNYSLPALSIEFLDFDTSRILCCIDRLSRLAPYRMNFSPGESQRWLWKT
                     WQRPETVTDILLHEGLPVRFGDLYFKAEN"
     gene            complement(2103..2849)
                     /gene="aat"
                     /locus_tag="PKM27_19095"
     CDS             complement(2103..2849)
                     /gene="aat"
                     /locus_tag="PKM27_19095"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNR09436.1"
                     /translation="MQQGTAPLFIPDSGKVIDFCSYPVESISGLDGLVAIGANFLPGT
                     LLTAYRCGLFPWFRYKGFAYWYCPDPRMVLMPSDLHISGTMQQVLRRELFKVTCDQAF
                     VEVMKACASMRRKHEDSTWIDQEYIEAYGRVHRQGFAHSFECWQEGKLVGGLYGLGMG
                     RIFFGESMFSFEKNASKTAFIQAVKFLEKSGFLLIDCQVPSAHLQSLGAVPISKKDYL
                     SKLKSLVASGPLDRQLWAGLFKPLKQDEMV"
     gene            complement(2859..3167)
                     /locus_tag="PKM27_19100"
     CDS             complement(2859..3167)
                     /locus_tag="PKM27_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNR09437.1"
                     /translation="MDNFSKTKWDEDILLDEGQVDLDDPALLVVYNDEVNTFDWVIQC
                     LMEVCSHTFEQSEQLSLLIHFKGKATVKTAPFDDLRPMKDALVERGLSAVIEGVKQTH
                     "
     gene            complement(3187..3651)
                     /locus_tag="PKM27_19105"
     CDS             complement(3187..3651)
                     /locus_tag="PKM27_19105"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNR09438.1"
                     /translation="MQIGSIAPHIALYNTERKKIDLNDYQGKKIVLFFFPAAWTGTCT
                     AEMCSIRDDYSYYEGLRAEVFGISVDSPLALKKFKEEQQIGFQLLSDFNKEVIRAYGV
                     YRQDFVCEMHGVAERSVFIIGSDGTIQYREVLENPGNLPDFPALKRALETIN"
     gene            3756..>4131
                     /locus_tag="PKM27_19110"
     CDS             3756..>4131
                     /locus_tag="PKM27_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HNR09439.1"
                     /translation="MSPLVRAYILLHLAVMLFGFTAILGALISLPALSLVWWRVGIAS
                     ISLLFFTRGFKFLTTLPSKQILRFSGIGAIIAVHWVSFFASVKLAGASVCLVCMATTC
                     FFLSLIEPLLIRKPFNRFDFFIA"
BASE COUNT         1020 a         1091 c          982 g         1038 t
ORIGIN      
        1 ggtggacatc gtaactattg gtcccgaggc aatccctgcg gaaaggatct atcaggatca
       61 atcctttttc aatagcttcc agaattttga ggtcagagag gatcatattt aaaaggctgc
      121 aaaaataggt gaatcttggg catcggatcc aaaagcagct tgatggccct gccacggcca
      181 ggagcgaaag cggaagcagt ttgcaagatt gccctaattt tacgccgcca tgccccagat
      241 cctccatcac gacgatgcca cgtaccacct cggggtctgg aaaatagaag agccggaatc
      301 ctttttcgtg gaccggctcc acgaagacca aaggcaattg cccttcctcc cggaccagcg
      361 acgccttcag tccctcgcag cccggttgct gctcaaccgg atgatggaaa gcccgcaggt
      421 gatccttaaa aacgaatcca acaaaccctt tttgagggat gatgaccggt atatctcgat
      481 tagccattcc tttgaagtgg cgaccgtact ggttgcccgc cggctctgcg gggtggatat
      541 cgaaaaaaac ctcgaccgga tagaacgtat agcggccaaa tttttattcc ccggggaaat
      601 ggaccggctc cagggcagcc agtaccgccg ccacctctac cgcatttggg gagcaaagga
      661 ggccatgttt aaggcctatg ggctgggagg tattgatttt aagaaagacc tgtggatcga
      721 tctggatgag atcctgaaca ataatgaagt attttcatcc cgtttactga atcatgaaag
      781 ggaaatccgt ttcgaaatgc gctggctgga gttgttgccg gactatatcc tggtatatgg
      841 ctccgaaatt tgacgggctg atggccttgg gttttatttt tctcctgttt gcatgcaaaa
      901 gctccccccg ccagcaagaa aattttccct taccccgcat acatgctttt gattcctttt
      961 atacctcttt ccattccgac agtgcttacc aaatgaggca tattctcttt cccctggaag
     1021 gcctgccgga agccatggat acagcccgct acggcgccgt ttattactgg gacaaagaaa
     1081 cctggcgtct acaccgtcct tttgatccgg cggacaccac ttttgtgaga cagttccaga
     1141 tcatcgactc caccctcatc cgcgaagtga tcgcgcacct gatgagcgga taccgcctgg
     1201 aaaggaggta ttccttcacc ggaaaggact ggcagttgat ttattatgca ccattgaggc
     1261 agccggtacg cattgaggtt gaaataccgg atcgttgaag tggatcagcg tgccggcgga
     1321 tgccaaaagt gaccaatccc gcgtgatccc attgcggcaa tctggaccct cttcaattct
     1381 cggccttgaa atataaatcc ccaaacctca ccggcaatcc ttcatgtaag aggatatccg
     1441 tcacggtttc gggcctttgc caggttttcc acaaccagcg ctggctttcg cccggtgaaa
     1501 aattcatccg gtagggcgcg agccgactca atctatcaat acaacaaagg atccgggagg
     1561 tatcgaaatc gagaaattcg atggacaagg cgggtaagct ataattcaag ccctggatga
     1621 tctcccattc aaatccttcg gcatcgatct tgcaaaaccg gggcaggcca tgccactcga
     1681 tcaattggtc cagggtatgt tgtctgacct ggatggtctc gtcccaggtc gccttgatcc
     1741 ggctgtagtg gttgatttgg ttttgccagt ccgtgctgcg ggcggtggaa agggtcgggc
     1801 tcctgcggct gatccggaaa tcgatggtcc cctcttccct tccgatggca taaggaagca
     1861 attcaaaccg gtcctgatgt ccaaatcgtt caaccagatt ttgcacacag agtggttgtg
     1921 gctcaacagc cactacccag gcacccaggt ccaaaaaagc ccggctgcgg ttgcccaggt
     1981 gggcccctat atcaaatgcc aggtcaccgg cttgcaggaa ctgcctgtaa aagcgggtca
     2041 gcctccggcg gttaaaaggt ttggcatagt acatgagcca ggacctcatc aacccatatt
     2101 tttcagacca tctcatcctg cttcaaaggt ttgaataacc ctgcccacaa ttgcctgtcc
     2161 aggggaccgg acgcaaccag cgatttcagt ttggacaggt aatctttttt actgattggg
     2221 acggcgccca ggctttgaag atgagcagag ggcacctgac agtcgatcaa cagaaagcct
     2281 gatttttcga ggaattttac ggcctgaata aaagccgttt tggaagcatt cttttcaaag
     2341 ctgaacatcg attccccaaa aaatatcctg cccataccca ggccgtataa tccgccaacc
     2401 agttttccct cctgccagca ttcaaaagaa tgggcaaatc cctggcgatg cacccggccg
     2461 taagcttcaa tatattcctg gtcgatccag gtcgaatctt catgctttcg ccgcatggag
     2521 gcgcaggctt tcatgacttc cacaaaggcc tggtcacagg tgaccttaaa aagctcacgc
     2581 ctgaggacct gctgcatcgt ccctgaaata tggagatctg aaggcatcag caccattctg
     2641 ggatctgggc agtaccagta agcgaaccct ttgtatctga accaggggaa aagaccacaa
     2701 cgataggccg tgagcagagt acccggtaaa aaattggccc caatagccac caagccatcc
     2761 agcccggaaa tcgactctac cgggtatgaa caaaaatcaa tgaccttgcc ggaatccgga
     2821 ataaacagcg gtgcagtgcc ttgctgcatt caggcccttc aatgggtttg ttttacccct
     2881 tcgatcactg ccgacaggcc cctttccacc agggcgtcct tcatgggccg cagatcgtcg
     2941 aagggcgcgg ttttaaccgt ggctttgccc ttaaaatgga tgagcaggga caattgttcc
     3001 gattgttcga aagtatggct gcatacctcc atgaggcatt ggatcaccca atcaaaggtg
     3061 ttgacctcat cgttgtaaac caccaggagg gcggggtcat cgagatccac ctgtccttcg
     3121 tccagcagga tgtcctcgtc ccattttgtt tttgagaaat tatccatttg accttagttg
     3181 agtaatttaa ttaatagttt ccaaagcccg tttcagcgcc ggaaaatcag gcaggttacc
     3241 cggattctct aacacctcac ggtattgaat ggttccatcc gaaccaatga taaacacgga
     3301 cctttccgcg accccatgca tctcacaaac gaaatcctgg cgatataccc cataagcccg
     3361 gatgacctct ttgttaaaat cgcttaaaag ctggaaaccg atttgctgtt cttccttaaa
     3421 ctttttcaaa gcaagtgggg aatccaccga aatcccaaac acttccgccc ggaggccttc
     3481 ataataagaa taatcatccc tgatggaaca catttcagcc gtacaggttc cggtccaggc
     3541 tgcaggaaaa aaaaacagga cgattttttt accctggtaa tcgtttaaat ctatcttttt
     3601 ccgttcggta ttgtataagg cgatatgggg tgcgatcgaa ccgatttgca tatgttctgc
     3661 aagtaaggca caaaattagg aaaatataat tcaaacgcct ttaattaaca tcttgcacaa
     3721 aagcatatat ttagtcgcct catcttgctt aatacatgtc accattggtc cgcgcctata
     3781 tccttcttca cctggccgtt atgttgtttg gtttcacggc catactcggg gccctgatca
     3841 gcctgccggc gctcagcctg gtctggtggc gtgtggggat tgccagcatc agccttcttt
     3901 tttttacccg gggattcaaa ttcctcacta cccttccgag taaacagata ctgcgatttt
     3961 cagggatagg ggcgatcatc gctgtgcact gggtaagttt ttttgcttcc gtaaaattgg
     4021 cgggagcctc ggtttgcctc gtctgtatgg cgaccacctg tttttttttg agtttgatcg
     4081 agcccctcct gatcagaaaa ccattcaacc gctttgattt ttttatcgcc t
//
LOCUS       DAOQXT010000195         3952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_23443_length_3952_cov_6.040801, whole genome shotgun sequence.
ACCESSION   DAOQXT010000195 DAOQXT010000000
VERSION     DAOQXT010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23443_length_3952_cov_6.040801"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1286
                     /gene="ribB"
                     /locus_tag="PKM27_19115"
     CDS             162..1286
                     /gene="ribB"
                     /locus_tag="PKM27_19115"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNR09440.1"
                     /translation="MSPNNLKLNTIEEAIHEIREGKVLIVVDNEDRENEGDFVCAAEC
                     ITPSIINFMATHGKGLICAPIDERRADELDLPLMVSANTAFHETAFTVSIDLIGHGCT
                     TGISAHDRATCIKALTDPGSKASDFARPGHIFPLRAKSGGVLRRNGHTEAAVDLARMA
                     GLYPAGVLVEILNQDGSMARLPELLEIARLHQLKIITIQDLVSYRLRHERLIERSFEK
                     EIHTPLGDFRVLIYRQSNGDEHLAIVKGFFDGEEAVPVRVHSSSESGDIYSVLFEGYA
                     HQIEKTMEFIHKEGKGVFLNLFHGEKKRPLSESIRNHGQPKSLAVEENEQQRDIGVGA
                     QILKDLGIRKIRLITSQPKRRIGLKAFDLEIVGHLDPNLL"
     gene            1390..2139
                     /locus_tag="PKM27_19120"
     CDS             1390..2139
                     /locus_tag="PKM27_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09441.1"
                     /translation="MLHQFIIAIGALAISKDFILSHRLWDNWKSMRWVHTASIIAMFY
                     FGIRMFGVIVSLFSGLGSLASGDPGQLKMGFNAGFFNSDFSFFDDDIYKYLFLFCSMS
                     LVYHFGGKTMEILYQVPYRPTFKTFIRSQARGFAFVFMCYILESVITGISSGLLSAFH
                     IGWFQPVFKFLVQSFFFGAVIMDALHEERGWNFKASIKHSLNHYSGIALVCGTVGLAL
                     SHVPVLGALLVGSVVSVATMIAMKRIETTRF"
     gene            complement(2220..3161)
                     /locus_tag="PKM27_19125"
     CDS             complement(2220..3161)
                     /locus_tag="PKM27_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNR09442.1"
                     /translation="MMVEAQLTDRLLMIRPANFGFNADTAATNAFQSPEQPSGKVPVA
                     QLARAEFDRLVELLRQEGVEVLVFEDTDFPVKPDAVFPNNWITTHADGSLITYPMHAP
                     NRRAERREDILEQLESRFEVSQRYAFESFEDQGLVLEGTGSMILDRVNHIVYACLSPR
                     TDIGLLDKFCLLKQYKKVVFHALDPEDKPVYHTNVIMALGTDTAVVCLDCIPDESEKK
                     ELSESLTRTGKTILEIGWDQVCCFAGNMLQVRNQDGKALWVMSETAKQSLTSAQRDLL
                     GLQSKLLASPIPTIEICGGGSVRCMLAEVFLREKNNR"
     gene            3388..>3952
                     /locus_tag="PKM27_19130"
     CDS             3388..>3952
                     /locus_tag="PKM27_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNR09443.1"
                     /translation="MAKVQINMKEGRAVHDENLIVGIDLGTTNSLVAYILDGQPVTVK
                     TNDREEALVPSIISIEEGQVLVGEAAKQRLVSNPGQTVYSVKRLMGKSLNDVLHIQPY
                     FSYDLKRDDPDALVRIRIGENYFTPIELSAEILKQLKSLAENHLKKNVTQAVITVPAY
                     FNDAQRQATRDAGRLAGLDVLRIINEPT"
BASE COUNT          937 a         1090 c          970 g          955 t
ORIGIN      
        1 cctccacact ggagggtctt tttataaaca cggtaagaac agcaaaatcc cctcactgag
       61 caggcgctca gatttttcct gcctgtccac ttttgcgccc ccgatgcctt ggcgggagcc
      121 ttttatggga aacaaatccg gtatccttac ctttgcagga aatgagccca aacaatctga
      181 aactgaatac catcgaagaa gcgatccacg agatccgcga aggaaaagta ctgatcgtcg
      241 tggacaatga agaccgtgaa aacgaaggcg actttgtctg cgccgcagaa tgcatcaccc
      301 cctccatcat caattttatg gccacccacg gcaagggcct gatctgtgcc cccatcgacg
      361 aacggagagc cgacgaactg gatttaccac tcatggtttc cgccaatacc gcttttcatg
      421 agacggcatt caccgtttcg atcgacctga tcgggcacgg ctgtaccaca ggcatatccg
      481 cccatgaccg tgctacctgc atcaaagccc tgaccgaccc ggggtccaag gccagcgatt
      541 ttgcccgtcc aggccatatt ttccccttgc gggccaagtc aggcggggtc ttgcggagaa
      601 acggacatac cgaagccgcg gtcgatctcg cccggatggc ggggttatac ccggcagggg
      661 tgctggttga gattctcaat caggacgggt ccatggccag gcttccggaa ttgctggaaa
      721 tagccaggct gcatcaattg aaaatcatca ccattcagga tctggtctcc taccggctcc
      781 ggcacgaacg cctgatcgaa agaagttttg aaaaagaaat tcatactccc ctgggcgatt
      841 tcagagtcct catctaccgg cagtccaatg gggacgaaca cctggcgatc gtaaaaggtt
      901 tttttgacgg agaggaagcc gtcccggtga gggtacactc ttcgtcggaa tccggggata
      961 tttacagtgt cctgtttgaa ggatatgccc atcaaattga aaaaaccatg gaattcatcc
     1021 ataaagaagg caagggcgtg ttcctcaacc tttttcatgg cgaaaaaaaa cgacccctct
     1081 cggaatccat ccggaaccat gggcagccga agtcgctggc cgttgaagaa aacgaacaac
     1141 aaagggatat cggggtcgga gcccagatcc tcaaggacct gggtatccgg aaaatccggc
     1201 tgatcaccag ccagcctaag cgccggattg gactcaaagc ttttgacctg gagatcgtcg
     1261 gccatctcga ccctaacctc ctctgaaagc atccggcctt tgtccgggat tttaatggct
     1321 tgatcgatat tgttattctc cggggaattt cttaaattac tttgcaggtt agtagattaa
     1381 cctgttgcta tgttacatca gtttataatt gccatcgggg cgctcgccat cagtaaagat
     1441 tttattctta gccaccgtct ttgggataac tggaagtcca tgcggtgggt gcacaccgcc
     1501 tccatcattg ccatgtttta tttcggcatc cgtatgttcg gggtgatcgt cagccttttc
     1561 agcggactgg gctccctggc ctcgggagac ccgggccagt tgaaaatggg ttttaatgcc
     1621 ggcttcttca attccgattt ctccttcttt gacgacgaca tatacaaata cctgttcctg
     1681 ttctgcagca tgtccctggt ctatcatttc ggcggcaaaa ccatggaaat cctctaccag
     1741 gtcccttacc gtcccacctt taagaccttt atccggtccc aggcgagggg cttcgccttt
     1801 gtttttatgt gttatatatt ggagagtgtg atcaccggca tttccagcgg attgttgtcg
     1861 gccttccaca ttggatggtt tcagcctgtt ttcaaattcc tggttcagag ttttttcttc
     1921 ggcgccgtga ttatggatgc cctgcatgaa gaaaggggct ggaatttcaa ggccagcatc
     1981 aaacattccc tcaatcatta ctccggcatc gccctggtct gcggcacggt cggacttgcc
     2041 ctttcccatg tccccgtcct gggagccctg ctggtaggtt cggtggtttc cgttgccacc
     2101 atgatcgcca tgaaaaggat cgaaacgacc aggttctgaa acccctgcag tttatactat
     2161 ttgtacaccc tgaaaattgt accccctcag ccaattccac aattggacaa ttcgacaatt
     2221 caacggttgt tcttttctct caaaaacacc tccgccagca tgcaccttac gctcccccct
     2281 ccacatattt ctatagtcgg tattggggat gccaggagtt ttgattgaag gccaagcagg
     2341 tcacgctgtg ctgaagtcag ggattgtttt gccgtttcgg acatgaccca aagggccttc
     2401 ccatcctggt tgcggacctg cagcatattg ccggcaaaac aacaaacctg gtcccaacca
     2461 atttccagga tggttttgcc ggtgcgggtg agcgattccg acaattcttt tttttcgctt
     2521 tcgtccggga tgcagtccag gcagaccacg gcggtatccg taccgagcgc catgatcacg
     2581 ttggtatggt aaaccggttt gtcttccggg tccagggcat ggaaaacaac ttttttatat
     2641 tgtttcaaca ggcagaattt atctagcaat ccgatatcgg tcctcgggct gaggcaggca
     2701 tatacgatgt gattcacccg gtccaggatc atgcttcccg tcccttccag gaccaagccc
     2761 tggtcttcaa aggattcaaa agcataacgc tggctcactt caaacctgga ctccagttgt
     2821 tccaggatgt cctcccgcct ttccgcacgc cggtttggcg catgcatcgg gtaagtgatc
     2881 aggctgccgt cggcatgcgt ggtgatccag ttgttgggga aaacggcatc gggttttacc
     2941 gggaagtccg tgtcttcaaa taccaatacc tccaccccct cctgacgcaa cagttcaacc
     3001 aggcggtcaa attcagcccg ggccaactgg gccacaggaa cctttccgga gggctgctcc
     3061 ggcgattgaa aggcattcgt agccgccgta tcggcattga atccaaaatt cgccggccgg
     3121 atcatcagca gccggtcagt caactgtgct tcaaccatca ttcacttgaa attagtgctg
     3181 tacataaatt ttacccgccg gtttggagca aggcgggtcg ccccttatga ttgaaagata
     3241 caaatgaatg cgtacaaacc caggatttgg ccgaagccag tgcttcaaac taaccactcc
     3301 ctgtaatgtg ggggtaggtt ttaactccgg cccccggcaa aggcggacga aatgcttaat
     3361 tcccccgatc tttgcaaaaa atttctaatg gcgaaagtcc agatcaatat gaaagaaggc
     3421 cgtgccgtgc acgatgaaaa tctcatcgtg ggcattgacc tgggcaccac caacagcctg
     3481 gtagcttata tcctggacgg ccaacccgtc accgtcaaaa ccaatgaccg ggaggaagcc
     3541 ctggtgccct ccatcatcag catagaggag ggacaagtcc tggttgggga ggccgctaaa
     3601 caacgcctgg tctccaatcc cggccaaacg gtttattccg taaaacggct catgggcaaa
     3661 agcctgaatg atgtgttgca tatccagcct tattttagtt atgatctgaa acgggatgac
     3721 cccgatgccc ttgtgaggat ccggatcggt gaaaactatt tcacccccat agaactcagc
     3781 gccgaaatcc tgaagcagct taaatccctg gccgaaaacc acctcaaaaa aaatgtaacc
     3841 caggccgtga tcaccgttcc cgcctatttc aacgacgccc agcgccaggc cacaagagac
     3901 gcgggccggc tggccggttt ggatgtgctg cggatcatca atgaacctac gg
//
LOCUS       DAOQXT010000196         3859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_24273_length_3859_cov_5.563091, whole genome shotgun sequence.
ACCESSION   DAOQXT010000196 DAOQXT010000000
VERSION     DAOQXT010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24273_length_3859_cov_5.563091"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1192)
                     /locus_tag="PKM27_19135"
     CDS             complement(197..1192)
                     /locus_tag="PKM27_19135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR09444.1"
                     /translation="MNNILINSLPFILAVVLIQCKAGNGEGPGDQGPPELLGLNEDIL
                     THPFEDSLYFIRGAYYYQNENYEEAVRDLEYAVSLDSLQPAYHHLLADAYLDGNRSYE
                     ALQTLKKASALFPQRIPTLLKLCEFQFLLKQYEASLASCQQIRQLDPTNAEAWFMAGL
                     NYRDLADTAQAISSLQRATTLDDRHTDAWIVLGQLWQNRDDELALNAFENALHTDSTN
                     LQALHSLATYWQDLGRFERALELYLHIIRLDPGYTGAFIQSGLIYFQQDSFSKAFEYF
                     DIACKQEPTLAAGYYYRGTSREALGDPEGAKSDYRQALRLDPDYPEATKALDLIN"
     gene            1228..1749
                     /locus_tag="PKM27_19140"
     CDS             1228..1749
                     /locus_tag="PKM27_19140"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR09445.1"
                     /translation="MRWRRPAEGEIEGHFINYADLIWEEDALQVLKEARHASRELMFS
                     LSLEQWDYAYQEGKWTIKEVWQHILDSERIFAYRALRIARNDQTPLPGFEQDDYILPS
                     KARERSVAGIIHEYDAVREATITLFEYMPAEALEHTGTASNKKLSARALAFIIAGHER
                     HHIRGLRENYGVK"
     assembly_gap    1979..2078
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2079..3073)
                     /locus_tag="PKM27_19145"
     CDS             complement(<2079..3073)
                     /locus_tag="PKM27_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNR09446.1"
                     /translation="MSKLKNVKGPAIFLAQFMGDQAPFNSLETVCRWAADLGYLGIQI
                     PTWDGRLIDLEKAAGSKAYCDDLKGKVSAHGLEITELSTHLQGQLVAVHPAYNELFDG
                     FAPDAVKGNPKARTDWAVQTLKHAAQASRNLGLKAHATFSGALIWHTVYPWPQRPKGL
                     VDAAFKELASRWLPILNEFDTCGIDLCYEIHPGEDLHDGISFERFLAATGNHKRCNIL
                     YDPSHFVLQHLDYLEYIDLYHPYIKMFHVKDAELNPSGRSGVYGGYADWVDRAGRFRS
                     LGDGQVDFKSIFSKLAQYGYEGWAVLEWECCIKDSGQGAKEGAPFIRDHIIRVTE"
     gene            complement(3126..3827)
                     /locus_tag="PKM27_19150"
     CDS             complement(3126..3827)
                     /locus_tag="PKM27_19150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR09447.1"
                     /translation="MRHSKILLFLFTVVVPFSCKVRQPEPPPMPSVVDSTPPRETIRT
                     DIPEVAPSLMNRVAWQKPDLILDKIGQDLAGKVVADIGAGPTGFFSLKLAREGARVIA
                     IDIDPAALSYIEKEKAKLDSAQKSLIQTRLARVDNPRLQKEEIDAILIVNTVAYLGNK
                     QTYLANLKNNLKPGGRIVIVDFKMKRLPEQIAPPKSERIYVDVVEEYLYRAGYKNIQV
                     DDQSLAYQYIITAEK"
BASE COUNT          863 a          933 c         1061 g          902 t
ORIGIN      
        1 caaagggata ttctttgagg gggtgggcat tcaccggcct ggcttttttt tcagcggagg
       61 cgaccggcgc cggggcttcg ggcgccgcgg ccggtttgca tgaaaagcca tacaggaata
      121 aaatcaacaa gcctaacagt cttaacatat tggatcagtt ttcaaaaata aggcaaattc
      181 cctgccgatt ccggatttag ttgatcaggt ccagggcttt ggttgcttcc gggtaatccg
      241 ggtccaatct caaggcctgc ctgtagtcgg actttgcgcc ttccggatca cccagggctt
      301 cccggctggt cccccggtaa taatacccgg cggccagggt gggttcctgt ttgcaggcga
      361 tgtcaaaata ttcaaaggct ttggaaaaag aatcctgctg gaaatagatc aggcctgact
      421 ggataaatgc gccggtatat ccaggatcaa gccggatgat gtgcaggtac aattccagag
      481 cccgttcaaa acggccaagg tcctgccaat aggtcgccag gctgtgcagg gcctggaggt
      541 tggtgctgtc ggtatggagc gcattttcaa aggcattcag agccagttcg tcatcccggt
      601 tttgccatag ttgtccaagg acgatccagg catccgtatg ccggtcatcc agcgtggtgg
      661 ccctctggag ggaggaaatg gcctgggcag tgtccgccag gtcgcggtaa ttaaggccgg
      721 ccataaacca ggcttcggca ttggtgggat ccagctggcg gatctgctgg cagcttgcca
      781 gggaggcttc gtattgtttg aggaggaatt gaaattcgca aagcttgagc agggtgggaa
      841 tcctttgcgg gaaaagagcc gaggcttttt tcagggtttg cagggcctca taagaccggt
      901 tgccgtcgag gtaggcatcg gccaggagat gatggtaggc aggctgaagg gaatccaggg
      961 aaacggcgta ttccaggtcc ctgaccgcct cttcataatt ttcattttga taataataag
     1021 cgccgcggat aaaatagagg ctgtcttcaa atggatgggt taggatgtct tcattaagcc
     1081 cgaggagttc aggcggtcct tgatctcccg gcccttctcc attgccggct ttgcattgaa
     1141 tgagcacaac cgccaggata aaggggagtg aatttatcag gatattgttc aaattgatgc
     1201 aatttagcgg ctacaaaatt aatggatatg agatggagac gtcccgctga aggggaaatc
     1261 gaaggccatt ttatcaatta tgccgacctg atctgggaag aggacgccct gcaggtcctg
     1321 aaagaggccc gccatgcatc ccgcgagctg atgttcagcc ttagcctgga gcagtgggat
     1381 tatgcctacc aggaaggcaa atggaccatc aaggaagtct ggcaacatat cctggattcc
     1441 gaacgcatct tcgcctaccg ggccctgcgc attgccagaa acgaccagac ccctttaccc
     1501 ggttttgaac aggatgatta tatccttccc tcgaaagcca gggagaggag tgtggccggg
     1561 atcatccacg aatacgatgc ggtccgtgaa gccaccatca ctctctttga atacatgccc
     1621 gctgaagccc tggaacatac cggtacggcc agcaataaaa agctctccgc ccgggcgctc
     1681 gccttcatca tcgcgggaca tgaacggcat catatccggg gactgaggga aaattatggg
     1741 gttaagtagt taagtagtta atcagttaat cagttaatca gttaagagca ttacaattca
     1801 acaattcaac aattccacaa ttcaacattc cgatgcatcg gaacagcaat tccgcctcat
     1861 aatcccatca cccgcttgtt gaacgcatcg tccgcaccgc ctcctgcaaa atcatcaaaa
     1921 gctctttcgg tcaccctgat gatgtggtcg cggatgaaag gcgcgccttc cttggctcnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ttcggtcacc ctgatgatgt
     2101 ggtcgcggat gaaaggcgcg ccttccttgg ctccctgtcc ggaatctttg atgcagcatt
     2161 cccattccag taccgcccag ccttcatagc cgtattgagc cagcttgctg aaaatggact
     2221 tgaaatcgac ctgaccatcg cccagggacc tgaacctgcc ggcacggtcc acccagtcgg
     2281 catagcctcc gtaaaccccg ctgcgccctg aaggattcag ttcggcgtcc ttgacgtgga
     2341 acattttaat atagggatga tataggtcta tatactccag gtaatccagg tgttggagca
     2401 caaaatggct ggggtcataa aggatattgc atcttttatg attgccggtc gctgccagga
     2461 agcgctcaaa actgatgcca tcatgcaggt cttcccccgg atggatttca taacaaaggt
     2521 ctatcccgca ggtatcaaac tcattgagga taggcagcca gcgggaagcc agttctttga
     2581 aagcggcatc gaccaggccc ttgggccgct ggggccaggg ataaacggta tgccagatga
     2641 gagcgccgga gaaggtagcg tgggctttga ggccaaggtt cctgctggct tgcgcagcgt
     2701 gcttaagcgt ttgaacggcc cagtccgtcc gggctttggg attgcccttg acggcatccg
     2761 gagcgaagcc gtcaaacaac tcgttgtagg caggatgtac agcaaccagc tggccctgga
     2821 ggtgggtgga cagttcggtg atttcgagtc cgtgagcaga taccttaccc ttaaggtcgt
     2881 cgcagtaagc cttgctgccg gcggctttct cgaggtcaat cagccttccg tcccaggtag
     2941 ggatctggat gcccaggtat ccgaggtcgg ccgcccaccg gcagacggtt tccaatgaat
     3001 tgaatggagc ctgatctccc ataaactggg ccaggaaaat ggccgggccc tttacatttt
     3061 tcagtttgct catattcgat gaatgtgcgg atgattctgc cgataaatat aaaacatgtc
     3121 acggattatt tttcagccgt gatgatgtat tgataagcca gggattggtc atcgacctgg
     3181 atatttttgt agcccgcccg gtaaaggtat tcttcgacga catccacgta gatccgttcg
     3241 gatttgggtg gggcgatctg ttcaggtaac cgcttcattt tgaagtccac gatcacgatc
     3301 ctgcctcccg gtttcaggtt gttttttaga ttggccaggt aggtttgttt atttcccagg
     3361 taagcgacgg tattgacgat gaggatagca tcgatttctt ctttttggag cctggggtta
     3421 tccacccggg ccagccgggt ttggatcagg cttttctggg cgctgtccag ctttgctttt
     3481 tctttttcaa tataggaaag ggcagccggg tcaatgtcga tggcgattac ccgggctcct
     3541 tccctggcga gtttcaggct gaaaaatccc gtggggccgg ctccgatgtc tgcgaccacc
     3601 ttaccggcaa ggtcctggcc gatcttatca aggatgaggt cgggtttttg ccaggctacc
     3661 cggttcatca ggctgggggc gacctccggg atatccgtcc tgatggtctc cctgggcgga
     3721 gtactgtcca caacagaggg cattggaggc ggttccggtt gccttacctt gcaggagaag
     3781 gggaccacga cggtgaaaag aaaaaggaga atttttgaat ggcgcatgag agagggtcaa
     3841 aagtacaaac agaatggaa
//
LOCUS       DAOQXT010000197         3733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_25519_length_3733_cov_6.303970, whole genome shotgun sequence.
ACCESSION   DAOQXT010000197 DAOQXT010000000
VERSION     DAOQXT010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25519_length_3733_cov_6.303970"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKM27_19155"
     CDS             complement(<1..560)
                     /locus_tag="PKM27_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09448.1"
                     /translation="MKKSIYTALLCLLSLGLAAQETIYPAPAHQGVIALTGGTIHIGN
                     GSIIERGTVLIRNSRIEAVGPNVSVPSGAKVIPVAGKQVYPGLIAASSNLGLTEIASV
                     RATSDFGELGAYNPSVKAIMAYNAESKVIPTLRSNGVLLAQVTPRGGVFSGTSSVVQL
                     DAWNWQDAAYKMNNGIHLNFPALVNRP"
     gene            complement(593..3631)
                     /locus_tag="PKM27_19160"
     CDS             complement(593..3631)
                     /locus_tag="PKM27_19160"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR09449.1"
                     /translation="MKRFSFFLPFLLFGAGLWAQETFPKNDIDFTKRECYAFTGATLV
                     RAAGQAPLTNATLVIRKGRVEAAGQNVSIPADAIVVDCSDKHIYPSFIDIYSDYGMPA
                     VPRPQQRGFGGPTQLDNDAKYPAGWNAAIRAENAAARVFQVNDARARDYRNNGFGLVN
                     SHIPDGIVRGSAALVSLANAKENLVMIRDRTTAHFSFSKGSSTQSYPTSLMGSIALLR
                     QTFLDAQWYKTKPASEGTNLSLAAFNELLNLPMVFESGDKWNDLRADKIAREFGIRFI
                     LLAGTNEYQRIAEIKASEAKLIVPLNFPSAQDVEDPNDARLVSLADMKHWEMAPGNPG
                     ALQNAGVSFALTMYGLRSDGPGAGSFTANLSKALKNGLTEEKALAALTSEPAAMLGIS
                     DLVGSLEAGKLANFLITSGPLFQDKTVIHQNWIQGSPYIIRQEGWADYRGDYTLAIND
                     ESYKLKITGDPGRLSAQLIGKDTIKANLKLTDKLVQLSFSQKADSSKITRLSGVITGD
                     QWNGTGQDAKGQWVRWKMNKAGDAVASSSSSRGESGRKQEEKPRVMYPFQGYGWTEMP
                     STQDLLIKNATVWTSERDGILKQTDVLVRGGKIARIGKNLSSDNARVIDGTGKHLTAG
                     IIDEHSHIGATGSINECSQSVTSEVRIADVINPDDINIYRQLSGGVTAVHVLHGSCNT
                     IGGQTQLIKLRWGRNAEEMKFAPWDGFIKFALGENVKRSSTTQNNRFPDTRMGVEQVL
                     DDAFLRATQYDKAGKDKRRDLELDALLEIINKQRFITCHSYVASEILALMSVAEKHGF
                     KVNTFTHILEGYKVADRMKQHGAAASSFSDWWAYKMEVQDAIPQSPYLMSRLGLNVAI
                     NSDDAEMARRLNQEAAKSIKYAGMAEEEAIKMVTINPATMLHVGDRTGSIKTGKDADL
                     VIWNEHPLSIYARAEKTIVDGIIYFDREKDAEMQARISEERGRLVQKMISAKNGGERT
                     ERPTPSFEEEAQCDLDHYHGKGLWHRLEQRMVEEVHTHED"
BASE COUNT          721 a          975 c         1081 g          956 t
ORIGIN      
        1 gggcgattga cgagggccgg gaaattgagg tgtatcccat tgttcatttt gtaggccgca
       61 tcctgccagt tccaggcatc gagctggacc acgctgctgg tcccgctgaa tacccctccc
      121 ctgggagtga cctgggccag caagaccccg ttgctccgaa gggtgggaat taccttggat
      181 tcggcattgt aggccatgat tgcttttacg ctgggattat aggcgcccag ttcaccaaag
      241 tctgaggtag ccctcacact ggcaatttca gtcaggccaa ggttggagga tgccgcgatc
      301 aggccgggat atacctgttt gccggccacc gggatcactt tggcacctga aggcacggag
      361 acgttcggtc ctacggcttc aatcctggaa ttccggatca ggacggtccc tctttcgatg
      421 atgctgccat tgccgatatg gatggtgccg ccggtaagcg cgatgactcc ctggtgggcg
      481 ggcgcgggat agatggtttc ctgggccgcc agtccaaggc taagcaggca aagcagggcg
      541 gtatagattg attttttcat tggattccgt ttaacttttt tttctcaagg gtttaatctt
      601 catgggtatg aacttcttcg accatccgct gttccagccg gtgccaaagg cctttgccgt
      661 ggtagtggtc gagatcgcat tgggcttctt cctcaaaaga cggagtgggg cgttccgtac
      721 gttctccccc attcttcgct gaaatcattt tctggaccag ccggcccctt tcctctgaga
      781 tccgggcctg catttccgcg tccttttccc ggtcaaagta gatgatcccg tccacgatgg
      841 ttttttcggc ccgggcatag atgctcaggg gatgctcatt ccagatcacc aggtccgcgt
      901 cttttccggt ctttatgctt ccggtgcggt cgccgacatg gagcatggtc gccgggttga
      961 tggtcaccat tttaatggcc tcctcttcgg ccatgcccgc atattttatg cttttggcgg
     1021 cctcctggtt gaggcgccgg gccatttctg cgtcgtcgct gttgatggct acgttgagcc
     1081 caagccggct catgaggtag ggactctggg gtatggcgtc ctgcacttcc attttatagg
     1141 cccaccagtc ggaaaaactg gaggctgccg ccccgtgttg tttcatcctg tcggccacct
     1201 tgtacccttc caggatatgg gtgaaggtat tgaccttaaa cccgtgtttt tcggctacgg
     1261 acatcagggc caggatttca ctggctacgt aggaatgaca ggtgataaaa cgttgtttat
     1321 tgatgatctc caggagggca tcgagttcca ggtcccttct tttatccttt ccggctttgt
     1381 catattgggt agcccggagg aaggcatcgt ccagtacctg ctccactccc atccgggtat
     1441 cgggaaaacg gttgttctgg gtagtactgc ttcgtttgac attttcaccc agggcaaatt
     1501 tgataaagcc gtcccagggc gcaaatttca tctcttccgc gttgcggccc catctcagct
     1561 tgatcagctg ggtctggccg ccgatggtat tacaacttcc atggaggacg tggacggccg
     1621 taaccccccc gcttagttgc cggtaaatat tgatatcgtc cggattgatc acatccgcaa
     1681 tccggacttc ggaggtgaca gattgggaac attcgttgat ggaacccgtg gcgccgatgt
     1741 ggctgtgttc gtcgatgatg ccggcggtca ggtgtttgcc cgtgccatcg atcacccggg
     1801 cattgtcgga ggaaaggttt ttcccgatgc gggctatttt acctccccgg acgaggacat
     1861 cggtttgttt caggatccca tccctttcgc tggtccacac ggtcgcattt tttataagga
     1921 ggtcctgggt tgacggcatt tcggtccagc cataaccctg gaaaggatac attacccggg
     1981 gcttttcttc ctgcttgcgg ccggattccc ccctggatga tgatgatgac gcgaccgcat
     2041 ctccggcctt gttcattttc caccggaccc actgcccttt cgcgtcctgg ccggtcccat
     2101 tccattggtc accggtgatc accccggaga ggcgggtgat tttggagctg tcggcttttt
     2161 gagaaaaaga aagctgtacc agtttatccg tcagtttaag gtttgccttt atggtatcct
     2221 tgccgatgag ttgagcggac aggcggccgg gatccccggt aattttcagt ttataggatt
     2281 cgtcgttgat ggccagggta tagtctcccc ggtaatcggc ccagccttcc tggcggatga
     2341 tgtaggggga tccctggatc cagttttgat gaatgaccgt tttgtcctgg aagagcgggc
     2401 ctgaggtgat caggaaattg gccagtttac cggcttcgag gctgccgacc aggtcggaga
     2461 tgcccagcat cgccgctggc tccgaagtca gggcggccag ggctttttct tcggtgagcc
     2521 cgtttttgag ggctttggac aggttggcgg taaaacttcc ggcgcccggg ccatcggaac
     2581 gcaggccata catggtgagg gcaaaagaga ccccggcatt ctgcaatgcg ccgggattac
     2641 cgggcgccat ttcccagtgt ttcatatcgg cgagggagac caggcgggca tcgtttgggt
     2701 cttccacgtc ctgggcgctg ggaaagttga ggggaacgat caatttggct tccgaagcct
     2761 tgatttccgc gatccgctgg tattcattgg taccggccag gagaataaag cgtattccga
     2821 attcacgggc tattttatcc gcccgcaggt cattccattt atcaccggat tcgaacacca
     2881 tcggcagatt cagtaattca ttgaaagccg ccagggacag gttggtgcct tcgcttgcag
     2941 gtttggtttt ataccattgg gcatcgagaa aagtctggcg aagaagggca atggaaccca
     3001 tcagcgaggt gggataactc tgggtggaac ttccttttga aaaagaaaaa tgggccgtgg
     3061 tccggtcccg gatcatcacc aggttttctt ttgcattggc caggctcacc agggcggctg
     3121 agccgcggac gatgccgtcc gggatatggc tgttgaccag gccaaacccg ttgttgcggt
     3181 aatccctggc ccttgcatca ttgacctgga ataccctggc cgccgcattt tccgcccgga
     3241 tggccgcatt ccagcctgcc gggtatttgg catcgttgtc caattgggtg ggtccgccaa
     3301 aacctctttg ctggggcctg ggcacagcgg gcatgccgta gtccgaatag atgtctataa
     3361 aggagggata gatgtgttta tcgctgcagt ccaccacgat cgcatcggcg ggtatgctca
     3421 cattctggcc tgcggcttcc accctgcctt tgcggatgac cagggtggca ttggtcagcg
     3481 gagcctgtcc ggcggcccgg accagggtag ccccggtaaa ggcataacat tctcttttgg
     3541 tgaaatcgat gtcatttttc ggaaaggtct cctgggccca aagtcccgct ccgaagagta
     3601 aaaaggggag aaaaaaggaa aatcttttca tatggtttca ataaaagata aatataggca
     3661 atatggtaga tacgagatac gagatacgag atgccagatg cgagatacga gatgcgagat
     3721 gcgagatgcg aga
//
LOCUS       DAOQXT010000198         3718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_25659_length_3718_cov_6.059514, whole genome shotgun sequence.
ACCESSION   DAOQXT010000198 DAOQXT010000000
VERSION     DAOQXT010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25659_length_3718_cov_6.059514"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="PKM27_19165"
     CDS             complement(<1..1542)
                     /locus_tag="PKM27_19165"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNR09450.1"
                     /translation="MKSEVQVILCFAAIFAVSCKKAGDHATPRQPKVYSIEQFYKTTN
                     VGAVEFNAEESALLVHSNVSGIFNGYELPVNGDSARALTSSSSESIFMIGYVPGSPGY
                     LYSADKGGNENDHIYLQVPGEEVRDLTPGEKVKAGFLQWARDRKSLFYTSNERDPRYF
                     DVYEMDITTWKPKLFYQNDLGYNIGSVSEDKNFLSLTESITTSNSNIHLVNLNSGKTT
                     NITPHEGDQANQALFFGKEGKYLFLLSDQDSEFQYLLSYDLVTGEKTPVYKPNWDVMY
                     AYESESGKYRVVGVNEDGKTAIHIFQQPENTELKNLDLGSLSINNVFFSKSDGKMAFG
                     AGSSSSPTNIFVHEFAENKTTQLTRNLSPEVDPANLVDASVIRFKSYDGLEIPCIQWI
                     PKQAHEGNKVPALVFVHGGPGGQTRQSYSSLIQYLVNQGYLVLGINNRGSSGYGKTFF
                     KMDDRKHGDADLRDCIAAKSYLASLPEVDTNKIGIIGGSYGGYMVMAALTFAPDAFEV
                     GVNIFG"
     gene            complement(1532..2173)
                     /locus_tag="PKM27_19170"
     CDS             complement(1532..2173)
                     /locus_tag="PKM27_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNR09451.1"
                     /translation="MKKRPVLIGITGGSGSGKTTFIRELRSMYTPEEVCIISQDDFYH
                     PRQEQKKDREGVINFDLPSSVDLKKFAESIRALMKGKTITLQEYHFNNKSKKPRTLQF
                     IPAPVIVVEGLFIFHIPALKSMLDLKIYIEAKENLKVIRRIKRDQVERNYPLDDVLYR
                     YEKHVLPAFERFILPHKDDVDLIINNNTSFSPALKVLQGYINQTIIKNKNHEK"
     gene            2313..3593
                     /locus_tag="PKM27_19175"
     CDS             2313..3593
                     /locus_tag="PKM27_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNR09452.1"
                     /translation="MDLLSILRALFGISVLLGICYAMSDHRKRIDWKLVASGLVLQLV
                     IAIAMLKVPFLRVVFEAVANFFVLMINSAGQSAKFLFGDLANGSPFGFAFLVLPTIIF
                     FSALSSVLYYYGILQRIVYGFAWVMNRTMRLSGAESLAAAANVFIGQTEAPLVVKPYL
                     EKMTRSEIMCLMTGGFATIAGGVFGAYVAMLGGDSLESQRYFGLHLLTASVISAPAAI
                     VAAKMIMPETEPEKVSSKIQISRDQAGANALDAISRGTTDGLKLAVNVGAMLLAFMAF
                     IYLFNHFFDWIGAITHLNETIKNATNGSYSGLSIQYMLGMICAPIAWLIGVPAQDMVA
                     VGQLIGEKTALNEFVAYSSLAQMKDTLAPASVVIATYALCGFSNFASIGIQIGGVGSI
                     APGQRQNLISLGLRAMIGGTIACLLTAAIARLLV"
BASE COUNT          865 a          912 c          917 g         1024 t
ORIGIN      
        1 gccaaaaata ttgaccccca cctcaaaggc atcgggggcg aaagtcaggg cggccatgac
       61 catgtacccg ccgtaagagc caccgatgat ccctatttta ttggtatcca cttcaggaag
      121 gctggccaga taggatttgg cggcgatgca atccctgagg tcggcatccc cgtgtttccg
      181 gtcatccatt ttaaaaaaag ttttgccata gccgctggat ccccggttat tgatcccaag
      241 gaccagataa ccctggttga ccaggtactg gatgagggaa gaataagact ggcgcgtctg
      301 gcctcccggg cctccatgaa caaataccag cgcaggcacc ttattgccct cgtgcgcctg
      361 tttagggatc cattgtatgc atgggatttc aagtccgtca taggatttga atctgatcac
      421 tgaggcatcg accaggttag ccggatcgac ctccggggac aggttgcggg tcagttgggt
      481 ggttttgttt tctgcaaatt cgtgtacaaa aatattggtt ggggagctcg aactgccggc
      541 tccaaaagcc atcttcccat cggatttaga aaaaaacaca ttattgatgc ttagggatcc
      601 caggtcaagg tttttaagtt ccgtgttttc aggttgttga aaaatatgga tggccgtctt
      661 gccatcttca tttaccccga ccacccggta tttcccggat tccgattcat aggcatacat
      721 cacatcccag ttgggcttat agaccggtgt tttttcaccc gtaaccaggt cataactcaa
      781 gaggtattga aactcagaat cctggtccga aagtaaaaac aaatatttgc cttccttgcc
      841 gaaaaacagg gcttggttgg cctgatcacc ctcgtgtgga gtgatattgg ttgtttttcc
      901 ggaattcaaa ttaaccaggt gaatattgct gttgcttgtg gtaatgcttt cggtcagact
      961 caggaagttt ttatcctcgg aaaccgaacc aatgttgtag cccaggtcat tttggtaaaa
     1021 cagtttcggc ttccaggtgg tgatatccat ttcataaaca tcaaaatagc gggggtcccg
     1081 ttcatttgaa gtatagaata aacttttcct gtccctggcc cactgaagga atccggcttt
     1141 gactttttca ccaggggtga ggtccctcac ttcttccccg ggaacctgca aataaatatg
     1201 gtcgttttca tttcctccct tatcggcaga ataaaggtat cccgggctcc cgggtacgta
     1261 accgatcata aaaatagatt cgctcgaaga ggaggtaagg gcccgtgccg aatctccgtt
     1321 gaccggcaat tcatacccgt tgaaaatgcc ggaaacattg gaatgcacaa ggagggcgga
     1381 ctcctctgcg ttaaattcca ccgctccaac attggtggtc ttatagaact gctcaatcga
     1441 atatactttg ggctgacggg gcgtggcgtg gtcaccggct tttttgcagg acaccgcgaa
     1501 gatggccgca aaacacagga tcacctgtac ttcacttttc atggttttta tttttgatga
     1561 tggtctggtt gatatatccc tgcagcactt taagggcggg agagaaggag gtattgttgt
     1621 tgatgatgag atccacatcg tccttatggg gcaggataaa ccgctcaaaa gccgggagga
     1681 catgcttctc ataacggtat aatacgtcgt ccagggggta atttctttcg acctggtccc
     1741 ttttgatcct gcggatgacc ttcaggtttt ctttggcctc gatatatatt ttcaggtcca
     1801 gcatggattt gagggcggga atatgaaaga taaacagccc ttccaccacg atgaccggag
     1861 cgggaataaa ctgaagggtc ctgggctttt tggacttatt gttgaaatgg tattcctgga
     1921 gtgtgatggt tttgcctttc atgagggccc ggatgctttc cgcgaatttt ttgagatcta
     1981 cggaggaggg cagatcaaaa ttgatcactc cctcccggtc ttttttttgc tcctggcggg
     2041 gatggtaaaa atcatcctgt gaaatgatac agacctcctc aggagtgtac atcgatctca
     2101 attccctgat aaaagtcgtc ttgccggaac cgctgcctcc tgtaatccca atcagtactg
     2161 gtcttttttt catgcccccc gaaggtatta aaaatgggca ggagaaccct ccggaaagct
     2221 ggaaatatat tttcaacatt tcgggggctg tcttccggag aattccggtg aatctggcct
     2281 tattttctat ctttcaagct caaaatgcaa tcatggatct actctccata ttaagggccc
     2341 tcttcggtat cagcgttttg ctgggtattt gttacgccat gagcgaccac cgcaaacgta
     2401 ttgactggaa actggtggcc agcggcctgg tgctccaact tgtaatagcc atcgcgatgc
     2461 tcaaagtccc cttcttgagg gtggttttcg aagcggtggc caattttttt gtgctgatga
     2521 tcaacagcgc cggccagtcc gcgaaattcc ttttcgggga tttggccaac ggctcgccct
     2581 tcggatttgc cttcctcgtc cttccaacca tcatcttttt ttcggccttg tcttcggtcc
     2641 tgtattatta cgggatcctt caaagaatcg tgtatggctt tgcctgggta atgaaccgga
     2701 ccatgcggct ttcaggggcc gaaagtcttg cagcagccgc caatgttttt atcggccaga
     2761 cggaagcccc cctggtcgta aaaccctacc tggaaaagat gacccgttct gaaatcatgt
     2821 gtttgatgac cggtggtttt gctaccatcg ccggcggggt atttggagca tacgtggcca
     2881 tgctgggcgg ggacagcctg gaatcccagc gttattttgg actgcacctg ctcaccgctt
     2941 cggtcatttc cgccccggcg gcgatcgtag ccgccaaaat gatcatgccg gaaacagagc
     3001 ccgagaaggt gagttcaaaa attcagatca gccgggacca ggcaggagcc aatgccctgg
     3061 acgcgatatc caggggcacg acggatggtc tcaagctggc agtgaatgtc ggggcgatgt
     3121 tgctggcttt tatggccttt atttacctgt tcaatcattt ttttgattgg atcggggcca
     3181 ttacccacct caatgaaacg atcaaaaatg caacaaacgg ctcctattcc ggtctcagca
     3241 tccaatatat gctcggcatg atttgcgctc cgatcgcctg gctcatagga gttcctgccc
     3301 aggacatggt cgccgtaggc caattgattg gagaaaaaac cgccttaaac gaatttgtag
     3361 cttattcctc ccttgcgcag atgaaagaca ctttggctcc cgcatccgta gtgattgcaa
     3421 cttacgctct ctgcggcttt tcaaatttcg cgtccatcgg catccagatc ggcggcgtgg
     3481 gttccattgc tccgggacaa agacaaaatc tcatcagcct gggattgcgg gccatgatcg
     3541 gcggtactat tgcctgcctt ctgaccgccg ccatcgcacg attgctcgtc tgatcaagga
     3601 aattttcctt ttagctccta aaaaaactag tgtattgatt cacccaacca ctgactactg
     3661 attactgatt attgactact gactattgac tattgactat tgactattga ctattgac
//
LOCUS       DAOQXT010000199         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_25854_length_3700_cov_5.029630, whole genome shotgun sequence.
ACCESSION   DAOQXT010000199 DAOQXT010000000
VERSION     DAOQXT010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25854_length_3700_cov_5.029630"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..1412
                     /locus_tag="PKM27_19180"
     CDS             327..1412
                     /locus_tag="PKM27_19180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR09453.1"
                     /translation="MIPSGKPFLPDLKIYEEYLSGIWARNHLTNHGPLVAELEQRLKA
                     FFGVKHFFYVNNGTLALQIAIKALDLKGEIITTPFSYVATTSSIVWSDLTPVFVDIDP
                     LDFTLDAERIESAISPVTSAILATHIYGIPCATNRIQEIAFRHNLKVIYDAAHAFGVE
                     IGGKGVLNFGDISTISFHATKLFHTVEGGGIATNNDELAHRINYMRNFGHQGYEDFYG
                     LGINGKNSEFHAAMGLCLFPHISTLLNRRKIQSEHYDKQLTLASSHLQKPRLPSGIRY
                     NYAYYPLIFETEETMLRVRDALENEAIYPRRYFYPALTRLPYMKTQPMSVAESVSGKV
                     LCLPLYHDLTLNQIGDICDIIASQLIR"
     gene            1416..2027
                     /locus_tag="PKM27_19185"
     CDS             1416..2027
                     /locus_tag="PKM27_19185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNR09454.1"
                     /translation="MVIAGAGGHAREILTEWCQLGDPSPLWLYDPTRKKPLNIYGFEV
                     LFEEDALRKALETDNRFILGVGDPLIREKFFRHFTALAAHPHALISKHALISPFEVVL
                     GQGLNIMANAYISASVSIGDGCLVNAQAHMHHGSRIGRFCTLSPGCKVLGQAIIDDFT
                     EIGSNAVVLPGLQIGRRVRIGAGAVVTKDVPGGVTVKGIPGRW"
     gene            2039..3187
                     /locus_tag="PKM27_19190"
     CDS             2039..3187
                     /locus_tag="PKM27_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09455.1"
                     /translation="MKHLFKKAVRRARFLFRFVVYSKARQNFYREQGIRAHQDQLLPS
                     RYPKSLKKLIVFTIPGGHWSKGEFISGGIISIVTFCEETAALFPLHRSATILCTLRNS
                     YLVSNFKNFDNQTDVYRFEQLNAQFPNLTSLLIHIPEYAVKKFRDDLTAGDWKWLKKI
                     PEVHLNVMNQNIRQMPSPGVIGGLSSITRNITVTTAHLKYCNQEQRDKIGMPLHHLSV
                     WISPEQYVFTPWEEKENILVLSPDQHPMKDAILKKLAFLPDLQIMTIHNLRYREYKAL
                     ISKAKWALTFGEGLDGYFIESIFSGAIGFAVFNHEFFTPDFGALETVYASYDQLEKKL
                     PGDLFSLNQKDVFTRYQQKQFALCSKYYNIQKYRENISLFYSGKYSFP"
BASE COUNT          955 a          884 c          879 g          982 t
ORIGIN      
        1 tggtggatag tgggtggtgg atggtggatg gtgggtggtg gggacgaagc ttggcttcgg
       61 ccgtgtggac actatgtgta gaggtcggag ctgagctcct cccgaacaag ttattggcag
      121 caatccaccc ctcccttgct tccaaatatt acacggccat agccaagttt tgtctaaaca
      181 tagaagagtt gaagacggaa ctgggctcat acacggccga atctaagctt cgttcaaaca
      241 gttaagcttc gtccagacaa gagcaattgc acaataaatt caggcccctt ggccggattg
      301 gatttatttc agacctttag atatcaatga ttcccagtgg taaacctttc ttaccggacc
      361 tcaaaatcta tgaagagtat ttgtccggca tatgggcaag gaatcattta accaaccatg
      421 gccccctcgt cgctgaactg gaacaaagac taaaggcatt ttttggggtc aaacattttt
      481 tttatgtaaa caacgggacc ctggctcttc aaatagccat caaagccctg gatttgaaag
      541 gagaaatcat caccactcca ttttcatacg tcgccaccac ttcctccatt gtctggtcag
      601 acctgactcc tgtatttgtg gatattgacc ctttggactt taccctggac gcggaacgca
      661 tcgaatctgc catttcacca gttacttccg ccattttggc cacccatatt tacggcatcc
      721 cttgcgcgac aaaccggata caggaaatcg cctttcgtca taatctgaag gtcatctatg
      781 acgccgccca tgctttcggc gtggaaatcg gcggtaaagg cgtcctcaat ttcggggata
      841 tatccactat cagttttcac gcaactaaat tgtttcatac ggttgaagga ggaggcattg
      901 ccacaaataa cgatgaactg gctcaccgga taaattacat gcgcaatttc ggccaccagg
      961 ggtacgagga cttctatggc cttggcatca acggtaaaaa ctctgaattt catgccgcca
     1021 tgggattatg tctctttccc cacatctcca cccttttgaa caggcgtaaa atccagtcgg
     1081 aacattatga caaacaactg acccttgcct caagccattt acaaaaacca aggctgcctt
     1141 caggcatccg gtataattac gcctattatc ccctgatctt tgaaacggaa gaaacaatgc
     1201 tgagggtgcg cgatgccctt gagaacgagg ctatttatcc aaggaggtat ttttatcccg
     1261 cattaacccg tttgccctat atgaagacac agcccatgtc ggtggcagag tccgtttccg
     1321 gaaaggtact ctgccttccc ctgtatcatg atctgaccct caatcagatc ggggatattt
     1381 gtgatattat tgcctctcaa ctgattcgat agattatggt tattgccggt gcgggtggcc
     1441 atgccaggga aattttaacg gaatggtgcc aacttggtga tccttctccc ctttggttgt
     1501 atgatccaac ccggaaaaag ccgttaaata tctatggttt tgaggttctg tttgaggagg
     1561 atgccttgcg gaaagccctt gaaacggaca atcgtttcat cctgggggtt ggcgatcccc
     1621 tcattcggga aaagtttttc aggcatttta ccgcattggc tgctcatccg catgccctga
     1681 tctcaaaaca cgcattaatc agtccgtttg aggtggtcct tggacagggt ctgaatatta
     1741 tggccaatgc ttacatttca gcttcggtct ccattgggga cggctgtctc gtgaatgcac
     1801 aagcccatat gcatcatggc agcaggattg ggcgattctg tacgttgtca ccgggttgca
     1861 aagtgctggg tcaggccatc attgatgatt ttacggaaat cgggagcaat gcggtggttt
     1921 tacccggcct gcagataggc aggagggtaa ggattggggc cggtgcggtg gtcaccaagg
     1981 atgtacccgg cggagtcacc gtaaaaggaa taccaggaag gtggtaaatg cataccctat
     2041 gaagcacttg tttaaaaaag ctgtgcggcg ggcaaggttc ctgttccgct ttgtggttta
     2101 ctccaaggca aggcaaaatt tttacaggga gcagggaata cgcgcccatc aagaccaatt
     2161 gctgccatcc agatatccga aatccttaaa aaaactcatt gtttttacca tacccggggg
     2221 gcattggtcc aaaggagaat ttatttccgg agggatcatt tccatcgtaa ccttttgcga
     2281 agaaacagct gcactatttc ccctgcaccg gtctgcaacg attctatgca ccctgaggaa
     2341 ttcttattta gtttcaaatt ttaagaattt tgacaaccag accgatgttt accgttttga
     2401 acagttgaat gctcaatttc ccaacctgac ctcgctcctg atccatatcc ccgaatatgc
     2461 agtaaaaaaa ttccgggatg acctgaccgc cggggattgg aaatggttga aaaaaatccc
     2521 tgaggtgcac ctcaatgtga tgaaccaaaa catccgccag atgccttccc ccggggtcat
     2581 tgggggcttg agttccatta cccggaatat aacggttacc accgcacatt taaagtactg
     2641 caaccaggaa caaagggata aaatcgggat gccgttgcat cacctgtccg tatggatcag
     2701 cccggaacaa tatgtcttta caccctggga agaaaaggag aacatcctcg ttctttctcc
     2761 ggatcagcac ccgatgaaag acgccatatt aaaaaaactg gccttcttgc ccgatttgca
     2821 gatcatgacc atccacaacc tcagatatcg ggaatacaag gccctgatct caaaggccaa
     2881 atgggccctg acctttggag aaggtctgga tgggtatttt attgaatcca tattcagcgg
     2941 cgccattggt tttgcggttt ttaaccatga attttttact cccgatttcg gagcattgga
     3001 aacggtgtat gcatcctatg accaactgga aaagaaattg cccggcgatc tattttcgtt
     3061 gaaccaaaag gacgttttca cccgatacca gcagaaacaa ttcgccttat gttcgaagta
     3121 ctataatatt caaaaataca gggaaaatat ttccttgttt tattcaggca aatacagttt
     3181 tccataggtt ggtgggtggt ggatggatag gacgaagctt tgcttcggcc gtgtagcaac
     3241 tgttgggacg gagtttaact tcggcccttg cagaaaccac aaatacggcg attggacgaa
     3301 gcttgacaaa ggcctcactg tttggacgaa gctgggcttc ggccgagtgg actcgtgtgt
     3361 aggatagccg aagctaagct tcgtccgaac aagggtcccg tcaggggccg gatatgggta
     3421 gaaaatttat tgtaggtaca tttcgtccca gccgggacga gattcgtctt catttcaatc
     3481 tctaccaata tgatgcctcg tggcacaaat cctgtccttt cccctttttc aaccttgctg
     3541 gggaggagct tagctccgac ccccggcttc acccctgttt tgacccggaa atattcgatt
     3601 gatgtattcg tcaaataccg gatggcctaa gctaagcttc gtccaaacaa gggtcccgtc
     3661 aggggccgga tatgggtaga aaatttattg taggtacatt
//
LOCUS       DAOQXT010000200         3634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_26527_length_3634_cov_7.153954, whole genome shotgun sequence.
ACCESSION   DAOQXT010000200 DAOQXT010000000
VERSION     DAOQXT010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26527_length_3634_cov_7.153954"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..1442
                     /gene="solA"
                     /locus_tag="PKM27_19195"
     CDS             300..1442
                     /gene="solA"
                     /locus_tag="PKM27_19195"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HNR09456.1"
                     /translation="MHYDVIVAGLGTMGAATCSYLAGRGLKVLGIEQFDIPHDLGSHS
                     GFTRIIRKAYYEHPDYVPLLQRSYDLWRQQEQKTGQTLYHETGILYLGEPSSSVLRGS
                     LESSRLYDIPVEKWEGGKVRARYPQFHFPAHWDVLWEPGAGYLDVNKSITGFCNTATE
                     AGAEIHTREKIEAWKDQGDHILLKTSRSHYTASRLILTAGPWTRDLTAGLPLRVTRQV
                     LAWLQLPDLERYDYPAFPCWFLHDPDQGMYYGFPVSRTGDPSEPRGIKMALHMPGDTG
                     DPDRLDREIRKEDKAIFRYFLDRYMPRANPVSIHYKTCMYTYCPDEHFLVDYLPGTDR
                     KVVVACGFSGHGFKFAPVIAEILTGLVLGETPNPSAAFLSLKRLDL"
     gene            1439..1795
                     /locus_tag="PKM27_19200"
     CDS             1439..1795
                     /locus_tag="PKM27_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09457.1"
                     /translation="MKRTIPDLEHKSQPLAHRRVFLRRIFSALGLALGILALLLFIGT
                     AGYHYLGDLGWIDAVYNASMILGGMGPVDRLTSPAAKLFASGYALLSGIVFIGLIGLL
                     LGPLFHRIMHRLHIEK"
     gene            1795..2973
                     /locus_tag="PKM27_19205"
     CDS             1795..2973
                     /locus_tag="PKM27_19205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNR09458.1"
                     /translation="MNLPSVQLHPGREASVLRNHHWIFSGAIRKLDGEVKDGDLVMVL
                     GSSGVILGYGHYSPSNITVRMLQFGPEPLADTFWKDKIASALELRRNLDLAGHPDTNA
                     YRLVHGEGDGLPGLVIDWYDGHAVIQCHSLGMYRAAGEIANALRELYGSELKTIYNKS
                     RESLGDAIGEGNAFLYGDTEGALVRENGHEFMVNWVTGQKTGFFLDQRENRKLCGALS
                     RGRTVLNLFAYTGGFSVYALSGGAAQVDSVDVSQTAIGLLQQNADRLPESSAIHRSFS
                     VPVNEFLKDYGLYDLIVCDPPAFAKSLGKRHQALIGYKNLNAKVLRKVKPGGFLLTFS
                     CSQVMDRELFQQTIMAAALESGRKVRILQHLGQGPDHPVNIFHPEGAYLKGLLLYVNE
                     "
     gene            3024..3311
                     /locus_tag="PKM27_19210"
     CDS             3024..3311
                     /locus_tag="PKM27_19210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR09459.1"
                     /translation="MTEEKIHLEQDGPKGRFFLLVGPDTAAEMFISFPGSNRLVIEHT
                     EVLPGHEGKGYGKRMIEHAVNYARENKLRVIPFCLYAREVLRKTKEYQDVL"
BASE COUNT          875 a          938 c          922 g          899 t
ORIGIN      
        1 ggaatcgaac ctgtatcaaa agtttaggaa acttctattc tatccattga actacagggc
       61 ctttttgcat ttcggagtgc aaatatcctt ttccttttgc agttttccaa tattttgatc
      121 ggtaatactg caccctggaa tggttaccct ttacccattc caccatattc caatcatcca
      181 aacttgctaa ctagctaact agctaacttg ccaacttgcc aacttgctaa cttgctaact
      241 tgctaacttg ccaactagct aactagctaa cttgctaact agctaactaa ctattccaca
      301 tgcattacga tgtcatcgtc gccggactgg gcaccatggg ggccgccacg tgcagttatc
      361 tggcaggccg gggacttaaa gtccttggga tagagcaatt tgacattccc cacgacctgg
      421 gttcgcacag cggtttcacc cggatcattc gcaaggccta ttatgaacac cccgactatg
      481 tgcccctcct gcaaagaagt tatgatctct ggaggcaaca ggaacaaaag actggtcaaa
      541 ccctttatca tgaaaccggc atcctctacc tgggtgaacc atcaagcagc gtgctccggg
      601 gaagcctgga atcatcccgt ttgtatgata tccctgttga aaaatgggaa ggcggaaaag
      661 tccgcgcccg gtatccgcaa ttccatttcc cggctcactg ggacgtcctt tgggaacccg
      721 gggcgggata cctcgatgtg aataaaagca ttaccggatt ttgtaatacg gctacagaag
      781 ccggggcaga gatccatacc agggaaaaga ttgaagcctg gaaggaccag ggagatcata
      841 tcttgctgaa gaccagccgc agccactata cggcctccag actgatcctc actgctgggc
      901 cctggacccg ggacctgacc gccggcttgc ctcttcgggt cacccggcaa gtacttgcct
      961 ggcttcaact gcctgatctt gagagatatg actacccggc cttcccctgt tggtttctgc
     1021 acgatccgga ccagggcatg tattacggct ttcccgtttc ccgtaccggt gatccttctg
     1081 aaccgcgggg catcaaaatg gccctgcata tgcccggaga taccggcgat cctgaccggc
     1141 tggaccgtga aatcagaaag gaagacaagg ccatattccg gtatttcctg gaccgttata
     1201 tgccccgggc caatccggtt tcgatccact ataaaacctg catgtacacc tactgccctg
     1261 atgaacattt tttagtcgat taccttccgg gaacagaccg gaaagtggtc gtggcctgtg
     1321 gtttcagcgg ccatggtttt aaatttgccc cggtgatcgc ggaaattttg accggcctcg
     1381 tactcggcga aaccccgaat ccttccgcgg ccttcctgag cctaaaacga ttggaccttt
     1441 gaaaagaacg atacccgatc tggaacacaa atcccaaccc ctggcccatc gccgggtatt
     1501 tttaaggagg attttttctg cccttggcct ggcgctgggt atcctggctt tattgctgtt
     1561 tatcggaacg gcgggttacc attacctggg cgatctcgga tggatcgacg ccgtttacaa
     1621 cgcatccatg atcctgggag ggatgggacc ggtggaccgg ctgaccagtc cggcagctaa
     1681 actgtttgcc tctggttatg ccctactttc aggtattgta tttattggcc tgattggttt
     1741 gttgctgggt ccgctgtttc accgtatcat gcaccgctta catattgaaa aataatgaat
     1801 cttccttcag tccaacttca tcccggaagg gaagcctctg tgctccggaa ccaccactgg
     1861 atcttttccg gggcgatccg aaaacttgat ggggaggtca aagacgggga cctggtcatg
     1921 gtgctaggat cttccggagt gatcctgggc tatgggcatt acagcccttc caatatcacg
     1981 gtccgtatgc tgcaatttgg cccggaaccc cttgcggaca ccttctggaa agacaagatt
     2041 gcctcggccc tcgagctgag aaggaatctg gacctggccg gtcatccgga taccaatgct
     2101 taccgcctgg tccatgggga gggggacggc ctgcccggcc tggtgatcga ttggtatgac
     2161 ggacatgccg taatccaatg ccatagcctg ggcatgtaca gggcggcagg ggaaatcgca
     2221 aacgccttgc gtgaactgta tggtagtgaa ttaaaaacca tatataataa aagcagggaa
     2281 tcgctgggtg atgccatcgg ggaaggcaat gcctttttat atggggatac ggaaggcgcc
     2341 ctggtccgtg aaaacgggca tgaattcatg gtcaactggg tcactggcca aaagaccggc
     2401 tttttccttg atcagaggga aaacaggaaa ttatgcgggg cgctcagccg tggccggacc
     2461 gtgctcaacc tctttgccta taccgggggt ttttccgttt acgctttgtc cggtggagcc
     2521 gctcaggtgg attctgtgga tgtttcccag accgccatcg gcttgctcca acagaatgcg
     2581 gaccggctgc ctgaaagttc agcaatacac cggtcgtttt cagtgcctgt aaatgaattt
     2641 ctcaaagact acggccttta cgacctgatc gtctgtgatc caccggcttt tgccaaaagc
     2701 ctgggtaaaa ggcaccaggc gctgatcggg tataaaaacc tcaatgcaaa agtcctgcgg
     2761 aaggttaaac ccggcggctt cttactgacc ttctcctgct cccaggtcat ggaccgcgaa
     2821 ttgtttcaac aaaccattat ggccgccgcg ctggaatcgg ggcgcaaagt caggattctc
     2881 caacacctgg gacaaggccc ggatcatccg gtcaatattt ttcacccgga aggggcttat
     2941 ttgaaaggtt tattgctgta tgtgaatgag taaagggacg actggaagtc atcccttcac
     3001 tattccttct taacttcgag accatgactg aagagaaaat acatttggaa caggatggcc
     3061 ccaagggccg ttttttcctc ctcgttggcc cggataccgc tgcggaaatg ttcatttctt
     3121 tcccgggctc caaccggctc gtcatcgaac atacggaagt gctcccggga cacgaaggaa
     3181 agggttatgg taaaagaatg atcgagcatg cggtaaacta tgcccgtgaa aataaattga
     3241 gggtcatccc gttttgcctt tatgcgcgcg aggttttaag gaaaacgaag gaataccagg
     3301 atgtcctgta atcaatactg cacgcaacga cacaaagtcc gacagaccgc ctctttgtgt
     3361 ccttatgtcc ttgtggaaaa agattgacgt ttttttaacc gcgaagagcg caaagttcgc
     3421 agaggatgcg cagagggatg ctgaaggtgg catattatat taatacttca tcttagtgtc
     3481 cttagtgtct ttgcgggaaa aattgactaa ttcataaccg cgaaggacgc gatgttcgca
     3541 gaggatgcgc agagggatgc gaatgacaca cacctcatgt gtaatagcca ctatttgatc
     3601 tgacagtcgt cagaaaatta ataatcagtc attg
//
LOCUS       DAOQXT010000201         3629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_26580_length_3629_cov_6.681030, whole genome shotgun sequence.
ACCESSION   DAOQXT010000201 DAOQXT010000000
VERSION     DAOQXT010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26580_length_3629_cov_6.681030"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1346)
                     /locus_tag="PKM27_19215"
     CDS             complement(300..1346)
                     /locus_tag="PKM27_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HNR09460.1"
                     /translation="MHFLAVRFRLMLALCPIFFLLLLSCKQEASPVITLEQYHQPHEA
                     GVRTGGVKMIPIKTTQGEFKVWTKRIGNNPRIKVLFLHGGPSSTHEYWECAESFFPSE
                     GIEFILYDQLGSFYSDQPTDSSLWTIDRFVEEVEQVRAALALDSTNFFLVGQSWGGIL
                     ALEYALRYQQHLKGLVISNMMSDCRAYDLYADEVLAPGMDPKVVDSVKWMEANGLYTD
                     PRYMELLMPNFYKKHLCRLEEWPDPVNRSFAHLNPSIYVLMQGPSEFGISGRLEHWDR
                     SADLNKIRVPAMTIGATYDTMDPEHMRWMSTQMLKGRFLLCPNGSHMAMWDDQEVYFE
                     GLIRFIRDVDLGRM"
     gene            1454..1792
                     /locus_tag="PKM27_19220"
     CDS             1454..1792
                     /locus_tag="PKM27_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09461.1"
                     /translation="MSNRILFTWVCIFALTMPQCDKNEALFKYQGEITGLDFRKCACL
                     TPRCGCCGAWVIEINQSAYYFMELPKESEISLSQDSSFPIPVRLNYITDPESCGPQGH
                     YILITEIERR"
     gene            1948..2598
                     /locus_tag="PKM27_19225"
     CDS             1948..2598
                     /locus_tag="PKM27_19225"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNR09462.1"
                     /translation="MRIRFLAISVGILLAAQGLAQGFVDSNLVQFSGMVVTDQKNEPL
                     PIPYAHVGVEGTRRGTYADRNGFFSLVVVKGENLIFSAVGFKKAYYKVPEDLPDNRYS
                     IFQILYADTLTLPETVIYPWPSRENFKMEFLALDVSDKMREIAAKNLADNAMANLRNS
                     LPRDGRETYGTYQQQVVQNYYYAGQYRPINLFNVVAWKKFFDAWKEGKFKKKKEED"
     gene            3141..3599
                     /locus_tag="PKM27_19230"
     CDS             3141..3599
                     /locus_tag="PKM27_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNR09463.1"
                     /translation="MQSKASRISGYLAELPEDRRQLVDRLRKIIKKNLPKGFQEVMSY
                     GMIGYVIPHKIYPAGYHCDPSLPLPFINLASQKNFVALYHMGLYSDKKLLDWFTGEWE
                     KATTSRLDMGKSCIRFKKPESIPYDLIGQLASKMTPEDWIKKYEAAKRNN"
BASE COUNT          987 a          839 c          852 g          951 t
ORIGIN      
        1 attatgcata gaaataaata tcataaaaga tttggaggat cgtagaaatt cgtcctaaca
       61 tgggaacacc cggtaattat tcggcgggaa tacttgaatt ggattttgat atcgcaggca
      121 tcaaaatgat ctcacaggat ttttgaagac tcaaatggca caaacaaatc acacggagga
      181 gaggagagca cggaggaaat atttaattca aattttaagc tcttttgata cctcatccct
      241 ccgtggcctt cgtgggctcc gtgcgcacat ccccttcgtg agctcagttc gaaaatccct
      301 cacatcctcc ccaaatccac atcccgtata aaccggatca gcccttcaaa ataaacttcc
      361 tgatcgtccc acatggccat atggcttcca ttgggacaga gcaaaaaccg gcctttgagc
      421 atttgggtgc tcatccaacg catgtgttcc gggtccatgg tatcgtaggt agcgcctatg
      481 gtcatggcag gaacgcggat cttattcaga tcggcggagc ggtcccaatg ttctaatctg
      541 ccgctgatgc cgaattcgga gggaccctgc atgagtacgt agatcgaagg gttaaggtgg
      601 gcaaaggatc ggtttaccgg gtccggccat tcttccaggc ggcaaaggtg ttttttataa
      661 aaattaggca tgagcagttc catatacctg gggtcggtat acaatccgtt tgcttccatc
      721 cattttacgg aatcgaccac ttttgggtcc atgcccgggg ccagaacctc atccgcataa
      781 aggtcgtagg cccggcagtc cgacatcata ttggaaatga ccaggccctt gaggtgttgc
      841 tggtaacgaa gggcatattc cagggcaagg ataccgcccc aactctgtcc aaccaggaaa
      901 aaattggtac tgtccagggc caaggccgca cgcacctgct cgacctcctc tacaaaacga
      961 tcgatggtcc agagcgagct gtcagtgggc tggtcggaat aaaatgagcc cagctgatca
     1021 tagagtataa attcaatgcc ttccgatggg aaaaaacttt cagcgcattc ccagtattcg
     1081 tgggttgagg acggccctcc gtgcaaaaac aggactttta tcctgggatt attcccgatg
     1141 cgtttagtcc aaactttaaa ttccccctgg gtggttttga ttgggatcat tttgactccc
     1201 ccggttcgaa ctcccgcttc gtggggctgg tgatattgtt ccagggtgat gactggactg
     1261 gcctcctgct tgcaggaaag gaggaggagg aagaaaatag ggcaaagggc cagcataagg
     1321 cggaaccgga cggctaagaa gtgcatgatc tgttgtttta accaaattta acacgcattc
     1381 tccgttaaga gcaaaggatg tccaacgatt tacagaggga taccgtcttt attgattgtc
     1441 aaagcatcaa aacatgagta accgtatcct ttttacctgg gtttgcattt ttgccctgac
     1501 catgccgcag tgcgacaaaa atgaggcttt atttaaatac cagggagaaa tcaccggcct
     1561 tgatttcagg aaatgtgcct gcctgacccc gcgatgcggt tgttgcggcg cctgggtcat
     1621 tgaaattaac caatcggctt attatttcat ggaattgcca aaggaaagcg aaatcagcct
     1681 gagtcaggac agcagcttcc ctatcccggt gcggctcaat tacatcaccg acccggaatc
     1741 ctgcggaccc cagggccact atatcctgat cacggagatc gaacgacgct gaaaggattg
     1801 tatcgaactg ctgcagccct ttattctttt taactccctg caatttgccg gcccccgtta
     1861 aataaccgtt aaacgcattt tttatcatga tcccgctgac caggcccatt ggcagatatg
     1921 gattattccg agtaatttgt agccgaaatg aggatcaggt ttttagcaat atctgttggg
     1981 atcctgttgg ccgcccaggg cctggcacag ggctttgtgg acagcaacct ggtgcaattc
     2041 agcggcatgg tggtgaccga tcagaaaaat gaacccctgc ctatccccta tgcccatgtc
     2101 ggggtggaag gaacgcgccg gggcacctat gccgaccgca atggtttttt ttccctcgta
     2161 gtggtcaaag gggaaaacct gattttttca gcagtgggtt tcaagaaagc ctattataaa
     2221 gtgcctgaag atttgccgga taaccgctat tccatttttc agatccttta cgccgatacc
     2281 cttaccttac ccgagacggt gatctatccc tggcccagcc gggaaaattt caaaatggaa
     2341 tttctggccc tcgatgtttc cgacaaaatg cgggagattg ccgccaaaaa ccttgcggac
     2401 aatgccatgg ccaatcttcg caattccctg ccccgcgatg gtcgggagac ctatggcacg
     2461 taccagcaac aggtggtcca gaattattat tacgcgggcc agtaccgccc catcaatctc
     2521 ttcaacgtgg tggcctggaa aaaattcttc gatgcctgga aagagggcaa gtttaaaaag
     2581 aagaaggaag aggattagtt cataagttca catgttaaca agttaacaag ttagcaagtt
     2641 ggcaagcacc aggatggttt cttgacaagg gggccattgc gataagattt cgttcctttt
     2701 tagctttaga gaagtcttcc ccaagccctt gagacttcaa tttaattaaa tctagctttt
     2761 taatataata ggctgttaat tccattaaca ccaaattaat attttgtttt tatagatatt
     2821 aacatttgat ttaaaattta attctaaaat atgcaacctt cgggcctaga tttgcgtctt
     2881 taatatcgtt atgaaatacc tctttttaca ggatttaaga tggatcaagt tggtcctctt
     2941 attcgccaca gttatgctga tggcttatgc cgtcgtacag gccaaaatct aaagtaaatc
     3001 ttttgggatt ctgagtaggg atgaagtgag gggatcctca cctctcctta cccacctgta
     3061 taatggtctt atccggccgg cctgcttttt ggactctgaa acccgaaaat ggtaaatttg
     3121 atttgtcaaa tcaaagacct atgcaatcca aagcaagtcg aatttccggg tacctggccg
     3181 aattgccgga agaccggcgc cagctggtcg atcggttgag aaaaatcatt aaaaaaaacc
     3241 tacccaaagg ttttcaggaa gtcatgtctt acgggatgat cggatatgtg attccccaca
     3301 aaatctaccc ggccggctat cattgtgatc cgtccctgcc gctgcccttt atcaacctgg
     3361 cttcccagaa aaattttgta gccctttacc acatgggatt gtattcagat aaaaaactgc
     3421 tggattggtt tacaggggaa tgggaaaaag ccacaacatc cagattggat atgggtaaaa
     3481 gttgtatccg gtttaaaaaa cccgaatcca ttccttatga tctgattggt caattggcga
     3541 gtaaaatgac cccggaggac tggattaaga agtatgaagc ggccaagaga aataattaaa
     3601 aattaaaaat taattaatta agggatgga
//
LOCUS       DAOQXT010000202         3284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_30726_length_3284_cov_5.159492, whole genome shotgun sequence.
ACCESSION   DAOQXT010000202 DAOQXT010000000
VERSION     DAOQXT010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30726_length_3284_cov_5.159492"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKM27_19235"
     CDS             <1..293
                     /locus_tag="PKM27_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09464.1"
                     /translation="DVEDAFGDWILGSGILYTSRMEAIDAIFELVISGLKAFREVHKG
                     YTVMDLRIARKWKPFTFNLILKNLLNEEYSSRPALMDAPRNITFKMDYRIEK"
     gene            complement(335..802)
                     /gene="lysM"
                     /locus_tag="PKM27_19240"
     CDS             complement(335..802)
                     /gene="lysM"
                     /locus_tag="PKM27_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNR09465.1"
                     /translation="MGLFSFLKGAGEKLFGKKKESAPAVDPAAVERERKISAMESYLM
                     GLGIEVDNLDLDMEGDKVTVYGQVKHQADREKLILALGNIEGVGSVDDRISVLVSEPE
                     AEFYEVKSGDSLSKIAKHFYGDPMRYPEIFEANKPMLKHPDKIYPGQMLRIPK"
     gene            984..>3284
                     /gene="secD"
                     /locus_tag="PKM27_19245"
     CDS             984..>3284
                     /gene="secD"
                     /locus_tag="PKM27_19245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNR09466.1"
                     /translation="MLGKGLVKFFFVLLAVVCIMQYIFMLPTGRIERKADKYALAAVD
                     SLPDSLDAHTVQKAARARFLDSISDEVVFSIPGIKKYTYQELKQQQLALGLDLKGGLN
                     TILQVDLKDFLLRLSRNSQDPDFLAALDNAEKRLQSDNRNFITIFGEEFQKVAKGKSL
                     ASIFQRDPGLRTEINLSTSDAAVLNILRTRANETVKLTFDRLVNRIDKFGVVQPNVSL
                     DAARDQISVELPGIENPERARRLLQSTAKLEFWDVYRAGDPGTTSPSILNTLVDADVL
                     LRKRMGEDTMSMDSTTRVDTLITPVYDSIGNVIDTTFTYDTVTVAADPLAGRGPLLRL
                     FTPNFVNQDRTSFTFSPAIMGVAEGKDRERVMALLTDPDIKALFPRELEFRWGAKPES
                     LTSTTDGENSNMYFLYAIKKKRGSDEPPLEGDRIVNAQPSTNRTGQTTVTLKMDNAGA
                     KVWADMTTKAAQDNNREVAICLDDEVVSAPSVNGPITGGDTEIMGAFTVQEATDLANI
                     LQVGKLPAQTKIIQESLIGPSLGQENIRNSLWAMGIGFGLVLIFMVYYYGRGGIISIL
                     ALVANLFFIIGALASLGTVLTLPGIAGIVLTMGMAVDANVIIYERIKEELRAGKSLLT
                     SISEGFKHSMSAILDSNVTTLLIGFVLIYFGIGPIKGFGTVLIVGIVFTVFTALLLSR
                     LMVDYWTSKGRQIDFWTKATQNAFSHFNVDWIGMRKKAYLISGVIILAGIISIFTRGF
                     DLGVDFKGGYSYNLNLGSTPVKSEDLR"
BASE COUNT          809 a          867 c          821 g          787 t
ORIGIN      
        1 tggatgtcga ggatgccttc ggcgactgga tcctggggag cggcatattg tacaccagcc
       61 ggatggaagc cattgatgcc atctttgaac tggtcatcag cggtctgaaa gcgttccggg
      121 aagtgcataa ggggtatacg gtcatggacc tgcgcattgc caggaaatgg aagcccttca
      181 cctttaacct gatcctgaaa aatctattaa atgaggaata ttcaagccgt ccggccctga
      241 tggacgcccc aagaaatatc acgttcaaaa tggattacag gatagaaaaa taaaaaaggc
      301 cccgggtggg gccttgatta tgcagtggct ggcatcattt tggtatcctg agcatctgtc
      361 ccggataaat tttatcggga tgtttaagca tgggtttgtt ggcctcgaaa atttccgggt
      421 aacgcatggg gtcaccatag aaatgcttcg ctattttgga taaggaatcc ccggatttta
      481 cttcataaaa ttccgcctca ggctcgctga ccaggaccga gatgcggtca tccaccgatc
      541 ccacgccttc aatattgccc agggcgagga tcagtttctc ccggtcggcc tggtgtttga
      601 cctggccata tacggtgacc ttatcccctt ccatatcaag gtcgaggttg tccacttcga
      661 tacccaggcc catcaggtaa ctttccatgg ccgaaatctt tctttccctt tccacggcag
      721 cagggtctac cgccggggct gattctttct ttttaccgaa aagcttctct ccggcacctt
      781 ttaaaaatga aaataatccc ataacactaa aattttattg tttaaaaaat ctaccctaaa
      841 gttaatcaca tttttggccc aaaccggcct tttgcttatc ccttatcttt accaaaaaaa
      901 gagcgggtcc cccgaccggg gtattcaggt aaaagcacta tatttgtgcc gctttttaaa
      961 tatatatcta caataattat aatatgttag gtaaaggttt agtgaagttt ttctttgtcc
     1021 tcctcgcggt ggtatgcatc atgcaatata ttttcatgct gccgaccgga cggattgaaa
     1081 gaaaagcaga caaatatgcg ctggccgccg tggattcatt gccggatagc ctggatgccc
     1141 acaccgtgca gaaagcagcc cgtgcccgct ttctggattc catctccgat gaagtggtgt
     1201 tcagcatccc ggggatcaaa aaatatacct accaggaact taaacaacag caactggcgc
     1261 tggggctgga cctgaaagga ggtttgaata ccatattaca ggtggatctc aaggactttc
     1321 ttctccggct gtccaggaac agccaggacc ctgacttcct cgccgccctg gataatgcgg
     1381 aaaaaagatt gcaaagcgat aaccggaact tcatcaccat tttcggggag gagtttcaga
     1441 aagtagccaa gggcaaatcc ctggcctcca ttttccagcg ggatcccgga ctccgcactg
     1501 aaatcaacct aagcacttcc gacgccgccg tgctcaatat tctgaggacc cgggccaatg
     1561 aaaccgtaaa actgactttc gaccgcctgg tcaaccgtat cgataaattc ggggtcgtcc
     1621 agcctaacgt ctctctggat gcagcccggg accagatttc cgtggaactg cccggcattg
     1681 agaatccgga acgggcccgc cgcttgcttc aaagcacggc taaactggag ttttgggatg
     1741 tataccgcgc cggtgacccg ggcacgacat ctccaagtat cctgaatacc ctggtggatg
     1801 ccgacgtgct gctcaggaaa aggatgggag aggatacgat gtccatggat tccaccacca
     1861 gggtggacac cctcatcacc ccggtctatg attccatagg caacgtaata gataccacct
     1921 tcacctatga tacggtcacc gtggccgcag atccgcttgc cggaagggga cccctgctgc
     1981 gcctgtttac tccaaatttt gtcaaccagg accgcaccag ttttactttc tccccggcca
     2041 tcatgggagt ggcggaaggc aaggaccggg aaagggtcat ggccctgctg accgatccgg
     2101 acatcaaagc cctgttcccc cgggaactgg aattccgttg gggggcaaaa ccggaaagcc
     2161 tgaccagcac cacggatgga gaaaattcaa atatgtattt tctctatgcc atcaagaaaa
     2221 aaagaggctc cgacgagccc cccctcgaag gggaccggat cgtaaatgca cagcccagca
     2281 ccaaccgcac cgggcagacc acggtcaccc tcaaaatgga caatgcgggc gccaaggtat
     2341 gggccgacat gaccaccaaa gccgcccagg acaacaaccg ggaggtcgcc atttgcctgg
     2401 atgacgaagt ggtatccgcc ccaagcgtca acggccccat caccgggggc gatacggaaa
     2461 tcatgggtgc tttcaccgtc caggaagcca ccgacctggc caatattctc caggttggaa
     2521 aattgcctgc ccagaccaaa atcatccagg agtcgctgat cggcccctcc ctgggacagg
     2581 aaaacatccg caatagcctt tgggccatgg ggataggttt tggcctggta ttgatcttca
     2641 tggtctatta ttacggcagg ggtggtatca tttccattct ggccctcgtg gccaacctgt
     2701 ttttcatcat cggcgccctc gcttccctgg gtacggtact cacccttccg ggtattgcag
     2761 gtatcgtatt gaccatgggt atggcggtgg atgccaacgt catcatctat gagcggatca
     2821 aggaagaact cagggcggga aaatcgctgc ttacttccat ctcggaagga ttcaaacatt
     2881 ccatgtccgc gatcctggac tccaacgtca cgaccttact gatcggtttt gtattgatct
     2941 attttgggat cggtcccatc aaaggattcg ggacggtatt gatcgttggt atcgtgttca
     3001 ccgtatttac agccttgtta ttgtccaggc tgatggtgga ctactggact tcaaaaggaa
     3061 ggcagatcga tttctggacc aaagccaccc aaaatgcgtt ctcgcatttt aatgtggatt
     3121 ggatcggcat gcggaaaaaa gcttacctga tctccggggt catcatcctc gccgggatta
     3181 tatccatttt cacccggggc tttgacctgg gcgtggattt taagggagga tattcttata
     3241 atctcaatct ggggagcacc cccgtgaaga gtgaggattt gcgt
//
LOCUS       DAOQXT010000203         3239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_31351_length_3239_cov_5.273869, whole genome shotgun sequence.
ACCESSION   DAOQXT010000203 DAOQXT010000000
VERSION     DAOQXT010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31351_length_3239_cov_5.273869"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            826..1227
                     /locus_tag="PKM27_19250"
     CDS             826..1227
                     /locus_tag="PKM27_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR09467.1"
                     /translation="MKQHLAHIALLVNDYDEAIQYYTQQLHFTLKEDTPLSETKRWVV
                     VAPPGSGQTSLLLARAADDYQAGFVGNQSGGRVFLFLHTDDFWRDYREMVVKNIEFVR
                     PPSVESYGTVAVFRDLYGNLWDLIQPAGTDD"
     gene            1389..3089
                     /locus_tag="PKM27_19255"
     CDS             1389..3089
                     /locus_tag="PKM27_19255"
                     /inference="COORDINATES: protein motif:HMM:NF008183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNR09468.1"
                     /translation="MPPTQKSSNATPYTSDPAWWKEAVVYQIYPRSFKDTDGDGIGDL
                     KGIIEKLDYIKSLGIDMVWLNPVYASPNKDNGYDISDYRDIMEEFGSMADFDSLLRGF
                     HERNIRVFMDLVVNHCSNQHAWFQSASRDRNSPYYRYFHWWPAEKGKPPYRWSIFDEK
                     GDAWEYNAATSSYYLHYFSDFQPDLNWENPALRQEIYKMMRFWLDKGIDGFRLDAIAF
                     CSKDTTWPALPEEYHGNWPLYYASGPHLHDYMQEMNREVFSKYDIATVAEAMGDVERV
                     MKFVDPARKELNMAYSFEAIDFGYLPNEYKMPDPKGFDLVQWKKIHAKWTDAFSERGW
                     GTIYLGNHDQPRMLSRWGDDRPEYRVLSSKMLTTFILSMRGTPYYYYGDEIGMDNIRF
                     DKIEDYKDIELLTNYAQVKAKGGDLKRFLEGMKISSRDNGRTPMQWNDKEQGGFTAGR
                     PWLKVNPNYPAVNVSVQENDPNSILNHFRKMVQLRKHNPVLIYGDWKLVDPENPSIFA
                     YSREWEGKKVLVVLNFKNTRATLRTDLDLSHARVWTGNYPDPPGKKELRPYEAVIWEL
                     "
BASE COUNT          877 a          749 c          791 g          822 t
ORIGIN      
        1 tggccaaaca aaggcgaggt tgcttcagtg cctgcccgac tgatcaataa gataacggtg
       61 tggtcaggcg ggcgtcccgc ctgaagcgtt ttagcagtat gtcataagag agtttaaaat
      121 acaatttcat attgcatttc tgatggcatt aaaatattgc gaaccagttt caggcgagga
      181 cgccctgaaa caacctcgtt tgtgctgatc attctataca ctaactggac aatatcttga
      241 atttttagac cccccaacca tagtccgcct cgccatccaa ggacggcaag ggaggtcaac
      301 ctaaatgggc acagatggaa accgggctta gatccttagc ccatggatgg atggcttttt
      361 cgattttggc tcctcgtctt gccgttgaaa attttgatcc ctgcgcctat tgtcacaaga
      421 gggatggttt gagtgatgcc agcgttgcca cttgggtaac gttgagtgct tcgaaatggc
      481 tgaagaaagc gctgatatcg taaacgtggt taaattcgat ttcaccgata ccataaaaat
      541 ccaacaaggt cttcctgatt ttctttgaca atctgacggt tcctcagttg gttcctttag
      601 gcgtcctcgc ctaaaggagt ataattatta accggtttca ggcggggacg ccagaaacaa
      661 gcattcaacg accttttaat ttaaactcag tacttaaagg ttctgatggt gccctgaagc
      721 tgtaattttg cctttatagt ttactgtttt aactgcaaac ggctattgta acttttctgt
      781 taccaaaaaa tcaaagcatc gctttaaaat gaatttgaaa taagcatgaa acaacatttg
      841 gcccatatcg ccttattggt aaatgactat gatgaagcca tacaatacta tactcagcag
      901 ctccacttta ccttaaagga ggataccccc ttgagtgaaa ccaaacgctg ggtagtggtg
      961 gctcctccgg gatccggcca aacttctttg ctcctggccc gggccgccga tgactaccag
     1021 gcgggctttg taggcaacca atccggcggc cgggtctttc tgttcctgca tacggacgat
     1081 ttctggaggg attaccggga gatggtggtt aaaaacatcg agtttgtaag acccccttcg
     1141 gtggaatcct atggtaccgt ggccgtgttc agagatcttt atgggaacct ctgggatttg
     1201 atacaaccgg ccggaaccga tgattgagaa aaccggattg aaataaatga aaagtctcca
     1261 atccatccca gctccgtgta tctcataaac agatggtatt tttagatatg aaatcattgc
     1321 cccggggaat tgctctttta atcatatcag gctgtctttt cccggcgtgt aaaccacccg
     1381 acccgaacat gcctccgacg caaaagtcat ccaatgcaac tccgtatacc agcgaccccg
     1441 cctggtggaa agaagccgtc gtgtaccaga tctatccccg aagctttaag gatacggacg
     1501 gagatggcat aggcgatttg aaaggcatca ttgaaaaact ggattatatc aaaagcctgg
     1561 gcattgacat ggtctggctg aatccggtct atgcctcccc caataaggac aacggatacg
     1621 atatcagcga ttaccgggat atcatggagg agtttggcag catggccgat ttcgacagtc
     1681 tgttgagggg ttttcatgaa cggaatatcc gggtattcat ggacctggtc gtcaaccatt
     1741 gcagcaatca acatgcctgg tttcaatccg ccagccggga ccgcaacagc ccctattacc
     1801 ggtattttca ctggtggccg gcggaaaaag gcaaaccgcc ttaccggtgg agcatatttg
     1861 acgaaaaagg tgatgcctgg gaatacaacg cggcgaccag ctcctattac ctgcattatt
     1921 tttcggattt tcaacccgac ctcaactggg agaacccggc tttgcggcag gaaatttata
     1981 aaatgatgcg tttctggctg gacaagggca ttgacggatt ccgtctggat gccattgcct
     2041 tttgttccaa agacacgacc tggccggcct taccggaaga atatcatgga aactggccct
     2101 tgtattatgc cagcggcccc catctgcatg actatatgca ggagatgaac cgcgaggtgt
     2161 tcagtaaata cgatatcgcc acagtggctg aagctatggg cgatgtggag cgggtgatga
     2221 aatttgtgga tcctgcacgg aaagaactga atatggccta ttcctttgaa gccatcgatt
     2281 ttggttatct gcccaatgaa tacaaaatgc ctgacccgaa aggatttgac ctggtgcaat
     2341 ggaaaaaaat tcatgccaaa tggacggatg ctttttcgga aaggggttgg ggcaccattt
     2401 acctgggcaa tcacgatcag ccgcgcatgc tcagccggtg gggcgacgat cgtcccgaat
     2461 atagggtcct ttcttctaaa atgctcacca ctttcatcct gagcatgcgg ggcacgccct
     2521 attattatta cggggacgaa atcggcatgg acaatatccg ctttgataaa atagaagatt
     2581 acaaagatat cgagctgctt accaactatg cccaggtgaa agcaaaagga ggcgacctga
     2641 aacggttttt ggaaggcatg aaaatttctt cccgcgacaa cggacgcacc cccatgcaat
     2701 ggaatgataa ggaacagggc ggattcaccg caggcaggcc ctggttaaag gtgaatccca
     2761 attatccggc cgtaaatgta tctgtacagg aaaacgaccc gaattcaata ctcaaccatt
     2821 tcagaaaaat ggtgcagttg cggaaacaca atccagtact gatctatggg gattggaaac
     2881 tggtcgatcc tgaaaaccct tctatttttg cttacagccg ggagtgggaa ggaaaaaaag
     2941 tattggtcgt tttgaatttc aaaaatacca gggccacgct ccggacggac ctggatctga
     3001 gtcatgccag ggtctggacc ggaaattatc cggatccgcc cggaaaaaag gaattgaggc
     3061 cctatgaggc ggtgatctgg gagctttaaa aggtatgaag acctggtccc gcatccggag
     3121 ggtttaaatc gttcattcgc atcccctgat ttttatatat tgtcttgaaa taacctttta
     3181 tacgaatgtc gatcatcaaa actccggatc agcgggtcag ggtatttatc agttctacg
//
LOCUS       DAOQXT010000204         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_31634_length_3218_cov_5.604173, whole genome shotgun sequence.
ACCESSION   DAOQXT010000204 DAOQXT010000000
VERSION     DAOQXT010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31634_length_3218_cov_5.604173"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..678
                     /gene="dcd"
                     /locus_tag="PKM27_19260"
     CDS             142..678
                     /gene="dcd"
                     /locus_tag="PKM27_19260"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNR09469.1"
                     /translation="MILSDLKILEAIEKGLIRIDPFRRDCLGTNSYDVHLGRYISVYA
                     DEELDARRHHRVKEMEIGPEGFLMQPGRLYLGVTEEYTETHHTVPFLEGKSSIGRLGI
                     DIHATAGKGDVGFCNYWTLELSCVQPVRIYAGMPIGQLIYFTVEGEIDRPYDKKIDAK
                     YNTRNPRPVESMMWKNKF"
     gene            complement(738..1034)
                     /locus_tag="PKM27_19265"
     CDS             complement(738..1034)
                     /locus_tag="PKM27_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNR09470.1"
                     /translation="MDIQVKELKMKMERGDDFVLIDVREPYEHEEFNIGGRLIPLGEL
                     PLVMESLPEDQEMEIVVYCRSGNRSGMAKYLMEQAGYKKVRNLLGGMLAWQEGL"
     gene            complement(1042..1854)
                     /locus_tag="PKM27_19270"
     CDS             complement(1042..1854)
                     /locus_tag="PKM27_19270"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNR09471.1"
                     /translation="MDTKTPLLKDAFVWRNILAIFLFLFGLLLLTAWWMKWYTRHGQK
                     VQLADFTGKSLQEATKLARKHDFQLIAMDSVFRVDRPGSIILQQTPEAGSFVKEDRKI
                     YVVVTKHNADLIPLSSLPTLYGRSFEVKKAELEKGFDIRSNVAGYVYDAGPVNYIMGV
                     IYQGDTILSASLEKKDARIAKGSVLDFILSTDTGAEIPVPDMVCMTYSAALFLLTSLG
                     LNIQEVEEDVIDDKNASFVIHQEPVFDPGVKMKSGDTMRVFLHSRKPDTCPN"
     gene            complement(1980..>3218)
                     /locus_tag="PKM27_19275"
     CDS             complement(1980..>3218)
                     /locus_tag="PKM27_19275"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HNR09472.1"
                     /translation="DYKIELSLDPGFRSLVWSRLVTGDTSAVPETELESNQIYFWRIL
                     PLQSCSSARLPFSVFHTLSLACKTYSAGDTPRLISATGTPTVTSNILVGDQIQLKQVR
                     IPNLAGTHEFVGDLSLWLKAPSGDSIILWARQCNNLSNFNLSLDDLAPIGITCPLTDR
                     KAHRPQDSLTKLTQSNTMGTWTLRVRDFNNGAGGSLDSWSLELCGSIPLSSGPIATTN
                     LPLDLFELRSAMLSTSRLNFTDPDSDPLNIRILLLQDPELGSLLKGSDTLVAGDFFTL
                     ADLQTGLVTYSAGLVPADTTERISLIAFDEKKNWSGRFEFKINLLNDVSIPIREESPE
                     SPFRLFPNPTNGNIYLENQGPEQAFYRWFDPAGRMIRSGIIPGNSRILLPTDSSFTSL
                     SFIQLLQHNKVRVFKVVHLR"
BASE COUNT          811 a          781 c          855 g          771 t
ORIGIN      
        1 ggaggatctg gggcatggcg gcgtaaaatt agggcaatct tgcaaactgc ttcctctttc
       61 gctcctggcc gaggcggggc cttgaagctg cttttggatc cgatgcccaa agattcttct
      121 atttttgcag ccttttaaaa gatgatcctc tctgacctca aaattctgga agctattgaa
      181 aaaggattga tccgaataga tccctttcgc agggattgcc ttgggaccaa tagttacgat
      241 gtccacctgg gacgctatat ttcagtatat gccgatgaag agctcgatgc acggcgtcac
      301 cacagggtaa aagaaatgga aatcggcccc gaaggattcc tgatgcagcc gggcaggctt
      361 tatctgggag ttaccgaaga atataccgaa acgcaccaca cggttccttt ccttgaaggc
      421 aaatccagca tcggccgtct tgggatcgac atccacgcga cggcgggcaa gggggatgtg
      481 ggcttctgca attattggac cttggagtta tcgtgcgtgc aaccggttcg tatctatgcc
      541 ggaatgccga tcggccagtt gatctatttt accgtggaag gggaaataga ccggccctat
      601 gataaaaaaa tcgatgccaa gtacaatacg cgaaatccga gaccggtgga atcgatgatg
      661 tggaaaaaca aattttaatt gaatagctaa gcccgtttct gaaagcgaaa accctcccaa
      721 ggcagggcat cgggagctta aagcccctcc tgccaggcca gcatgccgcc gagcaggttc
      781 cttacttttt tatacccggc ctgttccatc aggtattttg ccattccgct tcggttgccc
      841 gaccggcaat aaaccacgat ttccatctcc tggtcctccg ggaggctttc catgaccagg
      901 ggcaattccc cgagtgggat cagccgtccg ccgatattaa attcttcgtg ctcataaggt
      961 tcgcgaacgt cgatcaggac aaaatcatcg ccgcgctcca ttttcatttt aagttccttt
     1021 acttgaatat ccataaggcg atcagttggg acaggtatcc ggttttctgg aatgaaggaa
     1081 aacacgcatg gtatccccgc ttttcatctt gaccccggga tcgaataccg gttcctgatg
     1141 aatcacaaag ctggcatttt tatcgtcgat cacatcttct tccacctcct ggatattgag
     1201 accgaggctg gtaagcagga aaagggcggc ggaataggtc atgcatacca tgtctggtac
     1261 gggaatctca gccccggtat ccgttgaaag gataaaatcg aggacagaac ccttggcaat
     1321 ccgcgcatct tttttctcaa ggctggccga aagaatcgta tccccctgat aaatgacgcc
     1381 catgatataa tttaccggcc cggcgtcgta aacatatccc gcaacattgc tccggatgtc
     1441 aaaacccttt tccaattcgg cttttttcac ctcaaagctt ctgccataca gggtgggcag
     1501 ggaagaaagt gggatgaggt cagcattgtg tttggtgact acgacataaa ttttgcggtc
     1561 ttccttcacg aaagaacccg cctccggggt ttgctgcagg ataatggatc ccggccgatc
     1621 taccctgaac accgaatcca tcgcgatgag ttgaaaatca tgtttgcggg cgagtttcgt
     1681 ggcttcctga agcgatttac cggtgaaatc ggccaattgg actttctggc catgacgggt
     1741 ataccacttc atccaccagg cggtgagcaa aagcaagcca aataaaaata aaaagatggc
     1801 taggatattg cgccagacaa aggcgtcttt cagcagaggg gtcttggtat ccacgaactt
     1861 caaatcaggc cacaaaagta accacgtata tcggatttaa gcataagatt taatttaatg
     1921 taatgcaaat gcatggcatg ctgcaacctg tccttgggcc cggcatggca agaccgggat
     1981 catcgcaggt gaaccacctt gaataccctg actttattat gctgcaggag ttggatgaaa
     2041 gataaggaag taaaggagct gtccgtaggg agcagtatcc tgctgtttcc cgggataatt
     2101 ccggaccgga tcattctgcc cgccggatca aaccatcgat agaaggcttg ctccggccct
     2161 tggttttcaa ggtaaatatt gccgttggtc ggattgggaa aaaggcggaa gggggattcc
     2221 ggggattcct cccggatcgg gatgctcaca tcattgagga ggtttatttt aaattcgaac
     2281 ctgcccgacc aatttttttt ctcatcaaaa gcgatcaggg aaatccgttc agtggtgtct
     2341 gcagggacca ggcctgctga ataggtgacc aagccggtct ggagatcggc cagcgtaaaa
     2401 aaatcccctg caacgagggt atccgacccc ttcagcaaac tacccagttc aggatcttgc
     2461 aacaacagga tcctgatgtt tagaggatct gaatcgggat cggtgaagtt caacctggaa
     2521 gtgctgagca tcgcggacct caattcaaat aggtccaggg gcaggttggt cgttgcaatg
     2581 ggccctgaac tgaggggaat cgacccgcac aattccaagg accagctatc caggctgcct
     2641 cccgccccat tattgaagtc ccgcacgcga agggtccagg tacccatggt attggactgg
     2701 gtcagtttag tcagggaatc ctgcggccgg tgagcctttc ggtcagtcag agggcaggtg
     2761 ataccgattg gggcaaggtc gtcgaggctg aggttgaaat tgctcaggtt gttgcattgc
     2821 cttgcccaaa ggatgatcga gtcgccggat ggagccttga gccataagga aagatcgccc
     2881 acaaattcat gggtcccggc aagatttggg atccggacct gtttcaattg gatctgatcc
     2941 ccgaccagga tgtttgaagt cacggtaggg gttccggtgg ctgagatcag cctgggggtg
     3001 tccccggcag agtaggtttt acaagccagg gagagcgtat gaaagacgga aaaaggcagt
     3061 ctcgcgctgg aacaggattg cagcgggagg attctccaaa aatagatttg attgctttcc
     3121 agttccgttt caggtacggc tgaggtatct ccggtgacca gccgggacca taccagactt
     3181 ctgaaaccgg gatcaaggga taattcgatc ttataatc
//
LOCUS       DAOQXT010000205         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_33022_length_3127_cov_5.874674, whole genome shotgun sequence.
ACCESSION   DAOQXT010000205 DAOQXT010000000
VERSION     DAOQXT010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33022_length_3127_cov_5.874674"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            537..1802
                     /locus_tag="PKM27_19280"
     CDS             537..1802
                     /locus_tag="PKM27_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNR09473.1"
                     /translation="MDIFERIRANMGPLGQYADIAEGYYIFPKLTGEIGSRMMFNGQE
                     VICWSINNYLGLANHPEVRKIDAEASAEWGLAYPMGARLMTGETDQHIRLERELAAFV
                     KKEAGCLLNYGYQGMTSCIDSLVSRRDVIVYDAESHGCIIDGVRLHLGKRIAFEHNNI
                     ESFEKQLVRAVKMAEEGGGSVLVITEGVFGMRGDQGKLKEIVAFKSKYPFRLLVDDAH
                     GFGTLGPNGEGAGVEQGVQDEIDIYFGTFAKSMASIGAFIAGPPDIIKFLKYNMRSQV
                     FAKSLPMPLVVGGLKRLELIRTNPSLKQQLWKNVNALQNGLREAGFDIGITNTPVTPV
                     FLKGHPMEASQMVHDLRANHRIFCSMVIYPIIPKGEIILRLIPTAVHTQEDIDLTLAA
                     FKAISGKLQRGEYKAEVVNPLMQQEAAGA"
     gene            complement(1808..>3127)
                     /locus_tag="PKM27_19285"
     CDS             complement(1808..>3127)
                     /locus_tag="PKM27_19285"
                     /inference="COORDINATES: protein motif:HMM:NF036499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HNR09474.1"
                     /translation="YVGGPRNYKWVNTNPLPRVWEQMNLAWEYHARQIWIVNVGDIKP
                     MELPISFFLDHAWNPEAFGPRDVQPYIENWYSAFFGARHAKEMADLTSAYLKINGRRK
                     PELLDSRTNSLENYNEFQRVSDEYQDLLRRAEILNADLPASYRDAYFQLILHPVRACS
                     NLYQLYLASAKNNWYAGRKSLLANEWADRVKQFYKTDSLITLEYHKLGNGKWNHMMSQ
                     THIGYTYWQQPPRNRMPRVIYVDRDSAVSFEGKTPWTENAPPMTRQLKNKKHTEARDG
                     VISLAADAYTRAIHAPGAQWTLLPDHGRTGNAMTTFPVMAADIALNKGSPHLRYRVRV
                     RDTGTFTLYWYFSPTLNFHNDGGLKCAVSIDREAPQILDLNSEDHKVRTWEAWVANNI
                     ILKSSRHTISRKGKHTIKFWMMQPGPVLQKWVLDLGGMKASYLGPPE"
BASE COUNT          740 a          790 c          870 g          727 t
ORIGIN      
        1 aggttttgtt aagattgggt agtgcaatca tcctaaaaaa aaactccgtg gcctctgcgg
       61 actccgtgcg atccgacctg tctgagttgt ttcgcacgga ggaaaggagg gcaaggagac
      121 aggttttgtt aagattgggt agtgcaatca tcctaaaaaa aaactccgtg gcctccgcgg
      181 gctccgtgcg aaccgacctg tccgagttgt ttcgcacgga ggaaaggagg gcacggagac
      241 aggttttgtt aagattgggt agtgcaatca tcatacaaaa aaaactccgt ggactccgcg
      301 gactccgtgc gaaccgacct atccgaggta tttcgcacgg agacaggttt tgttaagatt
      361 gggtagtgca atcacataca aaaaaaaact ccgtggactc cgtgaactct gtgcgcacaa
      421 aaagccccag cggaccccgt gcgaacaaaa ggcctcagcc cgccccgtgc gaacagccat
      481 gagcgaaaaa cctgaccatt tcttattacc tttggccaaa atttttgagc ttaacaatgg
      541 atatttttga aagaatccgt gccaatatgg gcccgctcgg gcagtatgcg gatatcgccg
      601 aaggatatta tatcttccca aaactgaccg gtgaaatcgg ttcccgcatg atgttcaacg
      661 ggcaggaggt catctgttgg agtataaaca attacctggg cctggccaac catcccgagg
      721 taaggaaaat cgatgccgag gcctccgcgg agtggggttt ggcctatccc atgggggcac
      781 gactcatgac gggggagacc gaccagcaca tccggctgga gcgggaactg gccgccttcg
      841 tgaagaaaga agcgggttgc ctgctgaatt acggctacca gggcatgact tcctgcatcg
      901 actccctggt gagcaggcgc gatgtgatcg tctatgacgc cgaaagtcat ggttgcatca
      961 ttgacggggt caggctccac ctgggcaaac gcatagcctt tgaacacaac aatatcgaaa
     1021 gttttgaaaa acaactggtc cgggcggtca agatggccga agaaggcggc ggatccgtcc
     1081 tggtcatcac cgaaggggtt ttcggcatgc gcggcgacca gggcaaactc aaagaaatcg
     1141 tagcgttcaa gtccaaatat cccttccggc ttctggtgga cgatgcccat ggcttcggca
     1201 cccttggacc caacggggag ggcgccgggg tggagcaggg cgtgcaggat gagatcgata
     1261 tctatttcgg cacttttgcc aaatccatgg cttccattgg ggcctttatt gccggccctc
     1321 cggatatcat caagttcctc aagtacaata tgcggtccca ggtctttgcc aaatccctgc
     1381 ccatgccttt ggtcgtaggc gggctcaaac gcctggaact catccgcacc aatccctcgc
     1441 ttaaacaaca actctggaaa aatgtcaatg cccttcaaaa cgggctccgt gaggccggtt
     1501 ttgacatcgg gatcaccaat acccctgtca ctccggtatt cctgaaagga caccccatgg
     1561 aggcgtccca gatggtccat gacctcaggg ccaatcaccg gatattttgc agcatggtca
     1621 tttacccgat cattcccaaa ggtgaaatca ttcttcgcct gatcccgacg gcggtccata
     1681 cccaggagga tatcgacctg accctcgcgg cattcaaagc gatcagcgga aaattgcaaa
     1741 gaggtgaata caaggcggaa gtggtgaacc ccctgatgca gcaggaagct gcaggagcct
     1801 gaacagctta ttcaggcggc cccagataac ttgccttcat tcctcccagg tccagtaccc
     1861 atttttgtaa gaccggccct ggctgcatca tccagaattt aatggtatgt tttcctttcc
     1921 tggaaatggt atgcctgctt gacttgagga tgatgttgtt ggccacccaa gcttcccagg
     1981 tacggacttt gtggtcttcc gaattgagat ccaggatctg aggcgcttcg cgatcgatgg
     2041 agacggcgca tttcagaccg ccatcattgt ggaaattgag ggtgggtgaa aaataccaat
     2101 aaagggtaaa cgtgccggta tcccggacac gtacccggta ccggagatga ggcgatcctt
     2161 tattcagggc gatatccgcc gccattaccg ggaaggtggt catggcattg ccggttcgcc
     2221 catgatccgg aagaagggtc cactgggcgc ccggagcatg aatagccctg gtataggcgt
     2281 ccgctgcaag ggaaatcacg ccgtcccggg cttccgtatg ttttttgttt ttgagttgcc
     2341 tggtcattgg gggcgcgttt tccgtccatg gggttttacc ttcaaaggat accgccgaat
     2401 cccggtcaac atagatcacg cggggcatac ggttgcgcgg aggttgttgc cagtaagtat
     2461 aaccgatatg cgtctggctc atcatatggt tccatttgcc attgcccaac ttatggtatt
     2521 caagggtgat cagggaatcc gttttataaa attgtttgac ccggtctgcc cattcattgg
     2581 ccagcagtga ttttctgccc gcataccagt tgttctttgc cgatgccagg taaagttgat
     2641 agaggttgga acaggcccgg accggatgaa ggataagttg gaaataggcg tcccggtagg
     2701 aggcagggag gtctgcgttc agtatttcgg cgcgccggag caggtcctgg tattcatcgg
     2761 acacccgctg aaattcattg tagttttcca ggctgttggt gcgggaatca aggagttcgg
     2821 gtttccgtcg gccgttgatt ttcagataag cggaggtgag gtctgccatt tctttggcgt
     2881 gacgggcccc gaaaaaagcg gaataccaat tttcaatata aggctgaaca tccctggggc
     2941 caaaggcttc tggattccag gcatggtcca ggaaaaagga aatgggcaat tccatgggtt
     3001 tgatatcgcc cacattgacg atccagattt gccgggcatg atattcccag gccaggttca
     3061 tttgttccca aacccggggc aggggattgg tgtttaccca tttgtagttg cggggcccgc
     3121 ccacata
//
LOCUS       DAOQXT010000206         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_33525_length_3096_cov_6.348898, whole genome shotgun sequence.
ACCESSION   DAOQXT010000206 DAOQXT010000000
VERSION     DAOQXT010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33525_length_3096_cov_6.348898"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PKM27_19290"
     CDS             <1..438
                     /locus_tag="PKM27_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09475.1"
                     /translation="VSQNNNASGQTSREARLRANNLRNVARLDGNTLYVNDYLELNPH
                     EKIRNQHVSVTVSVPVGKSVKISKELHPVDGRLDETKNYNWWEMPGKLWTMTKNGLVC
                     PECEATESKPIGQEVHEAVKQALEESGNAVEEVKERINEQNQN"
     gene            664..1605
                     /locus_tag="PKM27_19295"
     CDS             664..1605
                     /locus_tag="PKM27_19295"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNR09476.1"
                     /translation="MNLQQLEYAVTLAREKSFSKAASRCFVTQATLSMMVKKLEKEIG
                     VVLFDRSRLPVSVTPFGQSIIQQAQKVLAEIKTITALAAEIRGEVDSEIRIGIIPTLA
                     PYLLPLFIKPFCDQYPNLKVDIKDLITDDIIDHLKEGSLDFGLCATPLHEAGIHEVPL
                     FYEEFLVYASSNEFLPNKKYVLPEHINPQHLWLLEEGHCMRNQLLNLCQLQEDHHYRI
                     QFHYRAGSIETLINLVDRCEGITVIPKLTTLGLSKEQMAKVREFASPKPVREISLVSH
                     QHNPKAKLMLQLKTSIRQLISSTVPIQHKGLRIVQIE"
     gene            1851..2291
                     /locus_tag="PKM27_19300"
     CDS             1851..2291
                     /locus_tag="PKM27_19300"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNR09477.1"
                     /translation="MTDQFYMEKALEQALAAYEADEVPVGAVLVCGDRIVGRGHNLTE
                     HLNDITSHAELIALSAASEFLGSKYLPDCTLFVTVEPCVMCAGALKWAQIGRLVYGAA
                     DEKYGFMKFGREILHPKTKVEYGLLEEPCSKLIKQFFKKKRDQA"
     gene            2396..2938
                     /locus_tag="PKM27_19305"
     CDS             2396..2938
                     /locus_tag="PKM27_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09478.1"
                     /translation="MKPFFYFLCLLGIASCAPGLSPYTQKLHEQYDWQESDLKKVQFY
                     LSDDIVLRRKKRDGKAEISGGQIRIIDGNRYEVVRFRRGTPGVLLFSPKENRLAISFE
                     DGRRSDSYLMFGPNPNANNRYVLLAADWTRQSGEVTYQGLKWETDARCAFAGLLVDLR
                     TAENHDSEARVVKGRTVKDK"
BASE COUNT          888 a          718 c          767 g          723 t
ORIGIN      
        1 gtcagccaaa acaacaatgc ttccgggcaa accagccggg aagccaggtt gagggctaat
       61 aatctgcgga acgtcgcacg cctggatgga aatacccttt atgtcaatga ttacctggaa
      121 ctgaaccctc acgaaaaaat ccggaatcag cacgtatcgg tcaccgtttc ggttccggta
      181 ggcaaaagcg taaaaatcag caaggaattg cacccggtgg atggccgcct ggatgaaact
      241 aaaaattata actggtggga aatgcccggt aaattatgga cgatgaccaa aaacggcctg
      301 gtctgtccgg aatgtgaagc cactgaatcc aaaccgatcg ggcaagaggt ccatgaagcg
      361 gttaagcagg ccttggagga aagtggtaac gcggtcgagg aagtcaagga aagaattaac
      421 gaacaaaatc agaattaaag accccggatg ccttttccaa ggtcctttta ggctgaatta
      481 tttagattgg tcttatcata tcaaaggttc tccggtcatg ccggagagcc tttttttatg
      541 cttgtttgag gcattcaacc cagccagccg ggtaaaccct tcaagaaaag gtaaaaggtc
      601 cgggaccttt caatcataaa tattatcaat gtttaatagc tttgtataaa cagcattgat
      661 aagatgaacc ttcaacaatt ggaatatgca gtcaccctgg cccgggaaaa aagtttcagc
      721 aaagcggcat cccggtgctt tgttacccag gccacgctca gcatgatggt aaaaaaactg
      781 gagaaagaaa tcggggtggt ccttttcgac cgcagcagac tgccggtgag tgtcacccct
      841 ttcgggcagt cgattatcca gcaggcccaa aaagtcctgg cggagataaa aaccatcacg
      901 gccctggccg ccgaaatccg gggagaggtg gatagcgaga tccggatcgg catcataccc
      961 accctggccc catacctcct gcccctgttt ataaaacctt tttgcgacca gtaccccaac
     1021 ctcaaagtgg atattaaaga tctgatcacg gacgatatca ttgaccacct taaagaagga
     1081 agtctcgatt tcgggctttg cgccacgccg ctgcacgaag ccggtatcca tgaggtgcct
     1141 ttattttatg aagaattcct ggtgtacgct tcctcaaacg aatttctgcc caataaaaaa
     1201 tatgtgcttc cggaacacat caacccccag cacttatggc tgctggaaga aggccattgc
     1261 atgagaaatc agttgctcaa cctgtgccag ttgcaggagg accaccacta ccggatccag
     1321 tttcattacc gggcgggaag catcgagacc ctcatcaacc tggtggaccg gtgcgaaggg
     1381 attaccgtta tccccaaact gaccaccctg ggcctgagca aagaacaaat ggctaaagtc
     1441 cgggaatttg catccccaaa accggtccgg gaaatcagcc tggtcagcca tcagcacaat
     1501 cccaaagcca aactcatgct ccagctcaaa accagcatca ggcagcttat ttcctcaacg
     1561 gtacccattc aacataaggg cttgagaata gtccagatag aataggatgg ataggattta
     1621 taggatttat aggatttata ggatgaatag gatttatagg atgaatagga tttataggat
     1681 ggataggatg gataggatgg ataggaactt aggattatta ggattgatag gaacttagga
     1741 ttattaggat tgataggatg ggtacaatgt aattttttga taccatttta taccttaatt
     1801 gacctcaatc acctaatttt caaatcctac taatcctact aatcctacta atgaccgatc
     1861 aattttatat ggaaaaagcc ctggagcagg ctttggctgc ctatgaggcg gacgaagtgc
     1921 cggtgggtgc ggtgctggtc tgcggtgacc gcatcgtagg cagggggcat aacctgacgg
     1981 aacatctgaa cgacatcact tcccatgcgg aattgatcgc cctcagcgct gcatcggaat
     2041 ttcttggatc caaatacctg cccgactgca ccctctttgt cactgtagaa ccctgcgtga
     2101 tgtgcgcggg tgccctgaaa tgggcccaga tcggaagatt ggtctatggg gctgccgacg
     2161 aaaaatacgg atttatgaaa ttcgggcggg aaatcctcca tccgaaaacc aaggtggaat
     2221 acggcctgct ggaagagccc tgcagcaagc tgattaaaca gttttttaag aagaagagag
     2281 atcaggcttg acccctcagc ccttaaaatt cgttaaatcc ctggttaaag agtattaata
     2341 cggtatcccc cggtcaaatt gaaagtctat tttgtaaaga cgattagaaa tgtatatgaa
     2401 accctttttc tacttcctgt gccttttggg tatagcttcc tgcgcgcccg gtttgagccc
     2461 ttatacccaa aagctgcacg agcaatacga ctggcaggaa tccgacctga aaaaagttca
     2521 gttctatctg agtgacgaca ttgtcctgag aaggaaaaaa cgggatggca aggctgaaat
     2581 ttcaggcggc cagatcagga tcattgacgg caaccggtat gaggtggtcc gtttcagaag
     2641 gggcacaccg ggtgtattgc ttttcagtcc gaaagaaaac cggctggcca tcagtttcga
     2701 agatggaaga aggtcagaca gttacctgat gtttggcccc aacccgaatg ccaacaaccg
     2761 ttatgtcctg ctggcagccg actggacccg gcaaagcggc gaggtgacct atcagggcct
     2821 gaaatgggaa accgatgccc gctgcgcttt tgctggcctt ttggtggacc ttcggaccgc
     2881 ggaaaaccat gattcagaag ccagggtggt caagggaagg acggtgaagg acaaataaaa
     2941 attaaggaat taaagaatta aaaattaagg ggaagccttt aattcattca ttctttaatt
     3001 ctttaattta ttttaaccag cggtccaggt aatcaacata tgtctgccac aaacggattt
     3061 ggctcaccgc gctgtaggct accccgtggt ggccgg
//
LOCUS       DAOQXT010000207         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_34131_length_3058_cov_5.438561, whole genome shotgun sequence.
ACCESSION   DAOQXT010000207 DAOQXT010000000
VERSION     DAOQXT010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34131_length_3058_cov_5.438561"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1972
                     /locus_tag="PKM27_19310"
     CDS             332..1972
                     /locus_tag="PKM27_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNR09479.1"
                     /translation="MKKTLIFFLLWSWCLPAVLMAQAKSALPLQTGEDVAVATTTSGK
                     VRGYIHNGVYTYKGIPYAQAKRFQAPQKPASWEGVRSSMTYGPVSPLMNPTTQVNDEV
                     EFVFHHDWGYTNEDCLRLNVWTKGLNDGKKRPVMFWIHGGGYTAGSSQELPSYDGEAI
                     SQKGDVVLVSINHRLNILGFLDLSDFGDQYAGSANASFYDLLAALQWVKENISSFGGD
                     PDNVTIFGQSGGGGKVSTLMSSPLAKGLFHKAIVQSGVWSDFQDQATSKRIGAAVLAE
                     LGLNASTVDSIQNIPYEKLVAAGNKALQKVREQLAAEGKPVGPGLRFGWGPTLDGKLL
                     PYHMSDPRALENCSDIPLIIGSTKNEFMASLRNPALKNANETEVKEFLKKQWGDKTEA
                     YISAVKKAYPKDTRPNDLMDIDAFFRPGMVAQANAKSAKGSAPVFLYLFTWQSPVMDG
                     AYKALHCMEIPFVMNNIARCEEMTGGGKEAYALADKMSQAWIQFARTGNPSHPGLPTW
                     PKYNEAVGATMMFDNVSVIRNHPDKEVLEMTMAVPARF"
     gene            2415..2489
                     /locus_tag="PKM27_19315"
     tRNA            2415..2489
                     /locus_tag="PKM27_19315"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2450..2452,aa:Val,seq:tac)
BASE COUNT          860 a          694 c          723 g          781 t
ORIGIN      
        1 gaatatttta acaatctggg aaacctgaaa aaattgcata aaacaaacat ataagggcgc
       61 caaggacgca gaggaggcgc aaagaaatac atacacacca atactctgcg ggatctttgc
      121 gttcttcgcg tcctttgcgg ttaatgatca ttttcttaac cgcaaagcat gcaatgaatc
      181 gcaaagaagg agccacaaag aaatacatgt acaccaatct tctgcgggaa ctttgcgctc
      241 ttcgcgtcct ttgcggttaa tgattgcaac ctgttttctg aaattccagg ttctgcttaa
      301 tttcacgaca attcttaata atattattga tatgaaaaaa acattaatct tttttctcct
      361 ttggtcatgg tgccttcctg cagtcctgat ggcacaggcg aaatccgcac tccctctgca
      421 aaccggagag gacgtagcgg ttgccacgac gacatcggga aaagtaaggg gctacatcca
      481 caatggagtt tatacctata aaggcatccc ttacgcccag gccaaacggt ttcaggcgcc
      541 tcaaaaaccg gcttcctggg aaggggtgag aagttcgatg acctatggtc cggtaagccc
      601 cctgatgaat ccgaccaccc aggtcaacga tgaggttgaa tttgtttttc atcacgactg
      661 gggctacacc aatgaagatt gcctgcggct gaatgtttgg accaaaggtt taaatgacgg
      721 caagaaaaga ccggtgatgt tctggataca tggtggcggg tataccgcag gttcttcaca
      781 ggaactgcct tcctacgatg gggaagcgat cagccaaaaa ggagacgtgg tcctggtgtc
      841 catcaatcac cggttgaaca tcctgggctt cctggacctt tccgatttcg gagatcaata
      901 cgccggatcg gccaatgcca gtttttatga cctcctcgcc gccctccagt gggtaaagga
      961 aaatatctcc agtttcggag gcgatcccga taatgtcacc atcttcggcc aatccggcgg
     1021 cggaggcaag gtctccaccc tgatgagttc acccctggcc aagggtttgt ttcataaagc
     1081 catcgtgcaa agcggggtat ggtcggattt ccaggaccag gctacctcca agcggattgg
     1141 agctgcggtg ctggcggaat tgggactgaa tgcatccacc gtcgactcca ttcagaatat
     1201 accttatgaa aaactcgtgg ctgccggcaa caaagccctg caaaaagtaa gagaacaact
     1261 ggccgccgaa ggtaagcccg tgggtcccgg actccgtttt ggctggggac cgaccctgga
     1321 cggtaaactg cttccttatc atatgagtga tcccagggct ctggaaaact gttctgatat
     1381 cccactgatc attgggtcca ctaaaaatga atttatggct tcccttcgca acccggctct
     1441 gaaaaacgct aacgaaacag aagtaaaaga atttttaaag aaacaatggg gagataaaac
     1501 cgaggcttac atatccgccg ttaaaaaagc ctatcccaag gatacccgtc ccaatgacct
     1561 gatggatatc gatgcttttt tcaggccggg catggtggcg caggccaatg ccaaatccgc
     1621 caagggttcc gctccggtct ttctttacct cttcacctgg caatcccccg tcatggatgg
     1681 cgcctacaaa gccctgcatt gtatggaaat cccctttgtc atgaacaata tcgcccgttg
     1741 tgaagagatg acgggaggag gcaaggaggc ttatgccctg gccgataaaa tgagccaggc
     1801 ctggattcag tttgcccgaa ctggtaatcc cagtcacccg ggtttaccta cctggccaaa
     1861 atacaatgag gctgttggtg ccacgatgat gtttgataat gtatcggtga tccggaatca
     1921 tccggacaag gaggttttgg agatgacgat ggcggtgccg gcgaggtttt gacagaatga
     1981 taaaatctat ttctcaatta ttccgaaagc cgcctttcca aaccttcagt ctcctctata
     2041 caattgggat cagtttcctc aataatatag tatataaacg taccgcaaca taatgctaaa
     2101 ctaacaccca aaaaaactac ctccatcagg ataaatccct atatttgccg ctcattttaa
     2161 gggagtttag ctcagccggt ttagagcatc ccgactgaag cgtcgggagg gtcggcggag
     2221 gtagaccccg gaaaagtaaa taatagggag tttagttcag ccggtttaga gcatcccgac
     2281 cgaagcgtcg ggagggtcgg cggaggtaga ccccggaaaa gtaaataata gggagtttag
     2341 ttcagccggt ttagagcatc ccgaccgaag cgtcgggagg gtcggcggag gtagacccgg
     2401 aaaagtaaat aatagggagt ttagctcagc cggtttagag catctgcctt acaagcagag
     2461 ggtcggcggt tcgatcccgt caactcccac tttgattatc agatggttat gaggattacc
     2521 acttcataac cattttttat ttgcaaacat tttgcaaaca atttagaaca ttcttatcat
     2581 gctcttcgta acagaaatat tctttttgtg acaggtgtga cggctgggac agggttttta
     2641 ttaaaacacc tggctgaata tccctcttca tttttcacta atacatgggg gtaccatttt
     2701 taaatatttg atgtcacaag tgtcacaggt gtcacagatt cagagcgaaa aggcatccat
     2761 tagctccaaa tcggatgatt tttcacaaaa atttaagact caattgaaat tctatatatc
     2821 tatttgatta ctaatggaat atgtaaataa ttattcctac ttgaaacttg acttaagttt
     2881 ataaaagatt ttttaatcaa tccaaaatct tttgatttaa cggtcaaaaa ttggaaagta
     2941 gaaccacatg ggggggtatt cgaaaaatcg gattgaccgg aggggaggag agatgtatct
     3001 tcctaaaacc gagcttatga atattttaaa gataattgta tagtacttgc ctttggat
//
LOCUS       DAOQXT010000208         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_34182_length_3055_cov_6.474333, whole genome shotgun sequence.
ACCESSION   DAOQXT010000208 DAOQXT010000000
VERSION     DAOQXT010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34182_length_3055_cov_6.474333"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2474)
                     /locus_tag="PKM27_19320"
     CDS             complement(<1..2474)
                     /locus_tag="PKM27_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09480.1"
                     /translation="MKVNAAFVITGLVSWMFLGFIGWYTNSDRYAGLLVKNAQNRLDQ
                     MAGLAREELGYGFRDAGDKLKEKDIILLAYHGDSIIYWNENQLEPEMVIGQSLSSFRE
                     FNGILYYFEKKAWDSLALLTGIPLRGISQNKPLVKSQAINLSSPDRKVSCLAFIERRL
                     PLWLEWTACLLGLLGLTTLTAAVVRKSLSLVSTQRLITALLVFCGGVFVLRILSLILM
                     DQVEITRIPFFDDSVKPSLLSPTLGDMIMNIGLLCLLIWFIRAHIRPSYFKRGQAISS
                     FGIAVINYWMVYVTAYLMVHIFKQLVMGSGLSFDFEYIFYLDIQSLVALAGILILAIA
                     LFVLDLHLMRIIRHLGMSPLYRLSANLAGLAGILPLIHLLPSHLPLLMLLALAGYLTL
                     LDLFVDHIRPHATWFLTWMVTGSILVSSLLYQFHIDKELQQRVALARQLKEEVLRVNP
                     DKDHLDQALQNQLSRLQPASPYLALYTVRYQPYRLPSGFEPLGAGLFEYKEPGSDTHS
                     VYVPLDPQAGFYLTKKSNQLFKAFSFFSYIFILFNLILLVFHLFNQRMRWFILPVFSF
                     FPSRFTLRYRIQLSMLGMSLGSFILIAIVIIFYLRRTSEGLALPGSVISYQSLQHEIS
                     RALNTNMAFNPQIKIYNPAGRQINSAVPERMPYPEYEAIQALDRLPYHTNSYHGYTKI
                     TLDGKSYFLGKNKTYTETNTKRRLNDILGTLLNVYVFLLILIVLISIVVANSITRPLT
                     ILSDKLKQIQIGGSNQKLEWNHDDELGGLIRNYNEMIQELDRSTRMLALTERETAWRE
                     MAKQVAHEIKNPLTPMKLITQHLQNS"
     gene            2635..3053
                     /gene="rnpB"
                     /locus_tag="PKM27_19325"
     ncRNA           2635..3053
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKM27_19325"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          777 a          682 c          895 g          701 t
ORIGIN      
        1 atggaattct ggaggtgctg ggtgatcagt ttcatggggg tcagcgggtt tttgatctca
       61 tgagccacct gcttcgccat ttcccgccag gcggtctctc tttccgtgag ggcaagcatc
      121 cgggtgctgc ggtccagctc ctggatcatt tcattgtaat tgcggatcag gccgcccaat
      181 tcatcgtcgt ggttccattc gagtttctgg ttgctgcctc cgatctggat ctgtttgagt
      241 ttgtcgctga ggatggtcag gggacgggtg atcgaattgg cgaccacgat ggagatgagc
      301 acgatcagga tcagcaggaa aacatataca ttgagcaggg tacccaggat atcgtttaac
      361 ctgcgcttgg tattggtctc cgtataggtt ttgttttttc ccaaaaaata ggactttccg
      421 tcgagggtga ttttggtgta cccgtgatag gaattggtat gatagggcag gcggtcaaga
      481 gcctggatgg cttcatattc gggatagggc atccgctccg gtacggcgct gttgatttgc
      541 cggccggcag gattgtatat tttgatttga ggattgaaag ccatattggt attgagcgcc
      601 cggctgatct catgttggag ggactgatag gagatcacgg atcccggcag ggccaggcct
      661 tccgaagtcc tcctcaggta gaaaatgatc acgatcgcga tcaggatgaa ggagcccagg
      721 ctcatgccca gcatgctcag ctggatccgg tagcgcagcg taaaccgcga aggaaaaaaa
      781 ctgaacaccg gtaatataaa ccaccgcatg cgctgattga acaagtggaa aaccagcagg
      841 atcaggttaa aaaggatgaa gatgtaggag aaaaaagaga aggccttaaa tagctggttt
      901 gattttttgg taaggtagaa tccagcttgc ggatcaagag ggacatagac cgaatgcgtg
      961 tcggagccgg gctccttgta ttcgaaaagc ccggcgccca gcggttcgaa tcccgatgga
     1021 agccggtagg gctggtacct caccgtataa agggcgaggt atggggaagc cggttgaagc
     1081 cgtgaaagct ggttttgaag ggcctgatcg aggtgatcct tatcgggatt gaccctgagc
     1141 acttcttctt tcagctggcg tgcaagggca accctttgtt ggagttcttt atcgatatga
     1201 aactggtaca gcagggaaga aaccaggatg gacccggtca ccatccaggt caggaaccag
     1261 gtggcatgcg gacggatatg atccacaaag aggtctagca gggtgaggta tccggccagg
     1321 gccagcaaca ttaaaagggg aaggtgcgaa ggcagcagat ggatgagggg taagatgccg
     1381 gccagcccgg ccaggttggc tgataagcgg tacaaggggg acatccccag gtgtctgatg
     1441 atccgcatca ggtgcaggtc caggacaaag agggcaatag ccaggatcaa aatgccggcc
     1501 agggcaacca ggctctggat atcgaggtaa aagatgtatt caaaatcaaa ggaaaggccc
     1561 gaacccatta cgagttgttt gaaaatatgg accatcaggt aggcggtcac atagaccatc
     1621 cagtaattga tcacggcaat gccaaaggac gagatggcct gcccgcgttt aaaataggag
     1681 ggcctgatat gcgcgcgtat gaaccagatc agcaggcaaa gcaaacctat attcatgatc
     1741 atgtccccga gggtaggact gagcagggaa ggtttaacgg aatcgtcgaa aaaagggatc
     1801 cttgtgattt caacctggtc catgaggatc aggctgagga ttcttaaaac aaagaccccg
     1861 ccgcagaaaa caaggagggc ggtgattaat ctttgagttg aaaccaggga aaggcttttt
     1921 ctgaccactg cggccgtgag ggtggtcagg cccagcaggc ccaggaggca ggccgtccat
     1981 tccaaccaca gcggcagcct tctttcaata aaagcaaggc aggatacctt tcgatcgggt
     2041 gaactcagat tgatcgcctg ggattttacc aaaggtttat tttgtgaaat cccgcggaga
     2101 gggatgccgg tcagcaaggc caggctgtcc caggcctttt tttcaaaata atacaggatc
     2161 ccgttgaatt ccctgaaact ggagagggac tggccaatga ccatctcggg ttcgagttgg
     2221 ttttcgttcc agtaaataat ggagtcccca tggtaggcga gcaggatgat gtccttttcc
     2281 ttcagtttgt cccccgcgtc ccggaaccca tatcccaatt cttcccgggc caggccggcc
     2341 atctggtcga gccggttttg cgcatttttg accaagaggc ctgcataccg gtccgaattg
     2401 gtataccatc cgatgaatcc caggaacatc cagctgacca atccggtgat gacaaaggcg
     2461 gcgttgactt tcatagggag gcaaaagctt cacaatatat tagttattaa tgatatgtgt
     2521 ttatatttac taatcaataa cttatatttt aattttgtat ataaaaaaaa tgcatgcatc
     2581 aaaagggggc cggaaagcaa cctttccggc caggcagtgg ttacctttgt tttggataca
     2641 ggccggtcta tcgctgcggg gcttgccccg gggaggaaag tccggacaat gcagggcgtc
     2701 acaccaccta atccgtgggt tccggagcag tccggaaaca gatcgtgtca cagaaaacaa
     2761 ccgccctggg gtgacccggg gtaagggtga aaacgtgcgg taagagcgca cgcgggaggg
     2821 atgcaaatcc gtcccgggat aaaccttgtg aattgaaatg ccatgtaaac cctggttcct
     2881 gctttccgga gctaagactt cggagggatg gagttgagcg gcccgttcaa ctgatttttc
     2941 atccagggag ggtaggcagc tggattcctg gttgtgaaat caggaactag ataaatgata
     3001 ggccctttct ttcggggaag gaacagaatc cggcttacag gcctgtgtct ttttt
//
LOCUS       DAOQXT010000209         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_34325_length_3047_cov_7.162767, whole genome shotgun sequence.
ACCESSION   DAOQXT010000209 DAOQXT010000000
VERSION     DAOQXT010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34325_length_3047_cov_7.162767"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKM27_19330"
     CDS             <1..358
                     /locus_tag="PKM27_19330"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNR09481.1"
                     /translation="VWSPKAAKNWEQFTGRVEQHFNRFDLYGWNIARSLYDAVATVRK
                     SGAKLMCELKTEMPGAEIHYTLDNTFPDAFTPRYTGAFEIPEGDVTLRFVTVKNGKIL
                     GRMVVVPRKDLAARAR"
     gene            358..1389
                     /locus_tag="PKM27_19335"
     CDS             358..1389
                     /locus_tag="PKM27_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNR09482.1"
                     /translation="MHTPDSPTFPVPDSALWLEKVKTELKGRTLSTADYTWDEVRISP
                     FEALPEGARSISERKNNMWKTGSYIDPATESPAALMKALNEGASVLYIEFSKGENWKN
                     WFKDVRLDWISLHLVFENTGMLDSFLEFPDRATRLKISGSVRVKQVDPVSLYKKYAQR
                     LPAMRFLGFDLEDLKTAKSMASLLVRLADTMENVSPEVKPGRLEEQVVIHVYGQNDML
                     LNISVLRALRLLWRQLFLSAGIENPSGLLLAGHIRIDKTASYDPRIRSSVIAASMLLG
                     GIDELFIEGTDEASSRYGRMVQHIFREEALLGVFPDPLSGSHVVEKLTGTIAEKVWEE
                     YLRLRFKLK"
     gene            1529..2593
                     /gene="meaB"
                     /locus_tag="PKM27_19340"
     CDS             1529..2593
                     /gene="meaB"
                     /locus_tag="PKM27_19340"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNR09483.1"
                     /translation="MLKILNEVKRELSGKINPKASKMTGKPAVEITIPSWAKKIKSGD
                     PVALARAITLVESSLPSDQEKASQLLSACSPGSGGSMRLGITGPPGVGKSSFIEYMGN
                     ALLARDSLKIAVLAIDPSSPAQGGSILGDKTRMQVLAQQEGVFIRPSPSRPGGGGIHD
                     QTLHSIQLCEYAGYDLIVVETVGIGQSELNIHDITDLTLLLIPPGGGDELQGIKRGIT
                     EIADLILVHKNDSGLQNLARATQNHYQQALKLPGGRGIQVFTVSSLTQKGFDRVWEQI
                     LKLAAERKASGEWKKNRTRQRIRWFHSLIKRQWAAELNRYHAGKPLQAVEKKIGSTTG
                     YIGALAQAYYRALKKKALKP"
     gene            2725..>3047
                     /locus_tag="PKM27_19345"
     CDS             2725..>3047
                     /locus_tag="PKM27_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09484.1"
                     /translation="MANFILIWWFVRLFGPSTTEFEYRAPLRVVGPPLEPAALFNSPV
                     HRQIALSGTFGELRPNHFHTGLDIKSQNGSTGEPVYAASKGFVSRIKISAYGYGRALY
                     IQHPNG"
BASE COUNT          802 a          781 c          771 g          693 t
ORIGIN      
        1 ggtatggagc cccaaagccg ccaagaactg ggaacaattt acaggcaggg tcgaacaaca
       61 tttcaatcga tttgaccttt acggatggaa tatcgcaaga tccctgtatg atgccgtcgc
      121 caccgtcagg aaatcagggg ctaaactgat gtgtgaacta aaaacggaaa tgcccggcgc
      181 cgaaatccat tacaccctgg acaatacgtt cccggatgcc tttaccccac gatataccgg
      241 cgcctttgaa atcccggaag gggatgtcac cctgcgattt gtcacggtca aaaacggtaa
      301 aatcctgggc cgaatggttg tagtaccaag aaaagacctg gctgcaaggg caaggtgatg
      361 catacgcccg attcaccaac ctttcctgtt ccggattccg ccctgtggct ggaaaaagtt
      421 aagactgaac tgaagggcag aaccctttcg accgcagatt atacctggga tgaagtccgg
      481 atctcccctt ttgaagccct gccggaaggg gcccgcagca tcagtgaaag aaaaaacaac
      541 atgtggaaga cgggcagcta catagatcct gcgaccgaaa gccccgccgc cctgatgaaa
      601 gccctgaatg aaggggcctc tgtcctgtat attgaatttt ccaaaggaga aaactggaaa
      661 aactggttta aagacgtgcg cctggattgg atcagcctcc acctggtttt tgaaaatacc
      721 gggatgctgg attccttttt ggaatttccg gacagggcta cccgcttaaa gatcagcgga
      781 tcggtccgag tcaaacaggt cgatccggta agcctttata agaagtatgc ccagcgcctc
      841 ccggccatgc gttttcttgg ttttgacctg gaggacttaa aaactgcgaa atccatggcc
      901 tccctcctgg tccgcctggc tgatacgatg gaaaacgtat cccctgaagt aaaaccgggc
      961 cgcctggagg agcaggtggt catccatgtt tacggccaga atgatatgct cttgaatatt
     1021 tcggtcctca gggccttgcg gcttttgtgg agacaattgt ttttatccgc cgggattgaa
     1081 aacccatccg ggctattgct tgccgggcat attagaattg ataaaacggc aagctacgat
     1141 ccgcgaatcc gttcctcggt catcgcagcc agcatgctcc tgggcggaat tgatgaactg
     1201 tttattgaag gaaccgatga ggcttcatcc aggtacggca gaatggttca gcatattttc
     1261 cgggaagaag cattattggg ggtttttccg gatcccctgt ccggatctca tgtggtggaa
     1321 aaactcaccg ggaccattgc cgaaaaagta tgggaagaat atttacggtt gagatttaaa
     1381 ttaaaataag gccggctgcg atgtaagcgt acatgcaaat tacccgaatc gttatcgagt
     1441 agccggcctt tctcccattg cttaaacata caatcaaaaa atatcgcgat tttactcccc
     1501 gattaatgga attaaccata tttatttttt gcttaaaata cttaacgaag tgaaaaggga
     1561 gctttccggc aagatcaatc caaaggcttc taaaatgacc ggaaagccgg ctgttgaaat
     1621 caccattcca tcctgggcaa aaaaaataaa atcgggggac ccggtggccc tcgccagggc
     1681 catcaccctc gtggaaagtt ccttgccttc cgaccaggaa aaagccagcc aactgcttag
     1741 cgcgtgcagc cccggttccg gcggcagcat gcgactgggg atcaccgggc cgcccggagt
     1801 gggcaaaagt tcctttattg aatatatggg caatgctctg ctggccaggg attccctgaa
     1861 aattgcagta ctggccattg acccgtccag ccctgcccag ggcggaagca tccttggtga
     1921 taaaacccgg atgcaggtcc ttgcgcagca agaaggggtc ttcatccgcc cctccccgtc
     1981 caggcccggc gggggaggca tccatgatca gaccttgcac agcatccagc tctgtgaata
     2041 cgccgggtac gacctcattg tcgtggaaac cgttgggatt ggtcagtcgg aattgaacat
     2101 tcacgacatt accgatctca cgctgttgct catcccaccg ggaggcgggg atgaactgca
     2161 gggcatcaag cgcggaatca ccgaaattgc cgatttgatc ctggttcata agaacgattc
     2221 cggcttgcaa aacctggcca gggcaaccca gaatcattac cagcaggcct taaagttgcc
     2281 tggcggccgg gggatccagg tattcacggt gtccagcctc acgcaaaaag gatttgaccg
     2341 ggtttgggaa cagattctga aactggccgc agaacgcaaa gcatcgggcg aatggaaaaa
     2401 aaaccggaca cggcagagaa tccgctggtt ccattccctt ataaaacgtc aatgggccgc
     2461 tgaactgaac cgctatcatg ccggaaaacc cctgcaagcc gtagaaaaaa aaatcggatc
     2521 gaccacaggc tatatcggag ccctggccca ggcttattac agggccttga aaaaaaaggc
     2581 cttgaagcct tgaagaccgg gcatcacgca tcaatgcctc atgaaccaag gaattttatt
     2641 ttaattttac aggcaacccc aggaatgtta aaaacacatt aaaaatattt ataatatatc
     2701 tttgccctct cgagggattt aaatatggcg aattttattc tcatctggtg gtttgtccgg
     2761 ctgttcggac cttcaacaac ggaatttgaa tatcgcgccc ccctgcgtgt ggtcggtcct
     2821 cccctggaac cggctgcctt attcaattcg ccggtccatc gtcaaatcgc tctctccgga
     2881 acttttggcg aactgaggcc taatcatttt cacaccggcc tggacatcaa gtcgcagaat
     2941 ggatccacgg gggaaccggt gtatgctgcc tccaaaggat ttgtcagccg catcaagatt
     3001 tctgcttatg gttatggaag ggcgctgtac atccagcatc cgaatgg
//
LOCUS       DAOQXT010000210         3044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_34369_length_3044_cov_7.112078, whole genome shotgun sequence.
ACCESSION   DAOQXT010000210 DAOQXT010000000
VERSION     DAOQXT010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34369_length_3044_cov_7.112078"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..951)
                     /locus_tag="PKM27_19350"
     CDS             complement(112..951)
                     /locus_tag="PKM27_19350"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR09485.1"
                     /translation="MKSILSLVLLLLFLCPVYGQQEISLWPNGAPGFESRKNEPTQAK
                     DWWVKNIHNPSVTLYQPSTVPVNGTSVLICPGGGHRELVFNAEGRDAAAYFNKLGITA
                     FVLKYRLAREENSPYTLDIHTRQDVNRAMRLIRSRAGEWALDPDKIGIMGFSAGGEVA
                     AMVAYTGAAQPEATDPTDRLRSKPDFQILIYPGPYGLPERVPSDAPPTFMLVANDDMC
                     CSQPVINLLTRYREAGRPIEAHIYAKGSHAFNMGLRSKLRTISSWPDRLTDWMVDNEI
                     IKN"
     gene            complement(953..2314)
                     /locus_tag="PKM27_19355"
     CDS             complement(953..2314)
                     /locus_tag="PKM27_19355"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNR09486.1"
                     /translation="MDPYLITNAYIKEPPTTLWGRFKFLGPGFILSASIVGSGELIAT
                     TILGAKAGFAAFWIIILSCLIKVAVQLQYGKHAIWTGETTVRAFNLLPGFRIGRGSWA
                     VWTVFFLQFLKIVQLGGMVGGTAIVLRMLFPGGSVFIWTVVVGLSAAILIYKDYYPIV
                     EKGSLVMIALFTLLTMASLYLLKNTEYAFTWMEVLRDLRLKLPPDQVMVAIGAFGITG
                     VAADETIAYNYWCIEKGYAAYTGPKGTGEKEISDRRRRAEGWVKVMYTDAIVAMIIYT
                     VVTAAFYLLGSAILYQRGEVPEGNALIEKIALIYTESLGPEVKSAYLVGAFFVLFSSV
                     FASLAAWTRVFSDIFGQLGWIRFSDQGQRKKTVAFLSFLFPSLWIFMFYTINLPVLMI
                     LSGGFVGSVMLLVVGFGAVHFRFFRGYEEQNGAGASLFFWLSLLSILFVAVYGMYSIV
                     QQL"
     gene            complement(2463..2966)
                     /locus_tag="PKM27_19360"
     CDS             complement(2463..2966)
                     /locus_tag="PKM27_19360"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNR09487.1"
                     /translation="MNPSSWWIGEPLEIISSGLKVRYQGPGSPGSILIRMPDGEIREA
                     PLKDIRESGEDQEEENGYEELPLPEDDRKTDRGEFGDQLDLHYEALIQNHTPTPGKSI
                     LEFQLEICRQFILESIQRKRPHIRIIHGRGSGVLKKEVEALLKNHPEVSIISFNPFSA
                     SVDVVLS"
BASE COUNT          785 a          783 c          750 g          726 t
ORIGIN      
        1 tttttaagtg tttttcaacc ttaaaaacgg tcaacctctg tcaggcattg acagtcagag
       61 gtcagaggtc agaggtcaga gcaaatcttc cgtttaattt ctttaattcc tttaattttt
      121 aattatttca ttatccacca tccagtccgt caatcgatca ggccaggaag agatggttct
      181 gagtttgctc cggagtccca tattaaatgc atgactgcct ttggcataga tatgggcttc
      241 aatcggcctt ccggcttccc ggtaccgcgt aagcagattg attacgggct gggagcaaca
      301 catatcgtca ttggccacca gcataaacgt cgggggcgca tcggagggca ctctttcagg
      361 taagccgtaa ggacctggat aaatcaggat ttggaaatca ggcttgctgc ggagccggtc
      421 ggtgggatcg gttgcttcgg gttgggctgc tcccgtatac gctaccatgg ccgctacttc
      481 gccgcctgcg gaaaaaccca taatgccaat cttgtccggg tccagtgccc attcaccagc
      541 ccggctgcgg atcagccgca tggcccggtt gacgtcctgc cgggtatgta tatccagggt
      601 atagggggaa ttttcttccc ttgccaggcg atattttaag acaaatgccg ttattccgag
      661 tttattgaaa tatgctgcgg catccctgcc ctctgcatta aaaaccagtt cacggtgccc
      721 tccgcccgga caaataagca ccgaagtacc gtttacgggt acggtcgaag gctgatataa
      781 agtcaccgag gggttgtgta tattttttac ccaccagtcc ttggcctgcg tgggttcatt
      841 cttgcgtgat tcaaagccag gggcgccatt gggccacaag gaaatttcct gttgtccata
      901 gacaggacag agaaagagga ggaggaggac aagggagagt attgatttca tattaaagtt
      961 gttggacaat actatacatg ccgtaaaccg ctacgaagag gatggatagt aggctgagcc
     1021 aaaaaaacaa actggctccg gccccatttt gttcttcgta accgcggaaa aagcggaaat
     1081 ggaccgcccc aaaacctacg actaaaagca tgacggaacc tacaaatccg ccagaaagga
     1141 tcatgagcac gggaagattt atggtgtaaa acataaaaat ccagagcgaa gggaataaaa
     1201 aagagaggaa ggccaccgtt ttttttcgct ggccctggtc ggaaaaacgg atccagccca
     1261 attgaccgaa aatatcgctg aagaccctgg tccacgcagc cagggaggca aagacgcttg
     1321 aaaacagtac gaagaaagcc cccaccaggt aagccgattt tacctccggc cccagggatt
     1381 ccgtatagat cagggcaatt ttttcaatca gggcatttcc ttccgggact tcaccccgct
     1441 ggtagaggat cgctgaaccc aggaggtaga aggcagcggt caccacggta tagatgatca
     1501 tggccacgat ggcatcggtg tacatcacct tcacccagcc ttcggccctc cgtctccgat
     1561 cggagatttc cttttccccc gttcctttcg gaccggtata agcggcatag cctttttcaa
     1621 tgcaccagta attgtaggca atcgtttcat cggcagccac cccggtaatg ccgaaggcgc
     1681 caatggcgac catgacctgg tcaggtggca gcttcaaccg gaggtcgcgg aggacttcca
     1741 tccaggtaaa agcatattcc gtatttttca acagatacaa agaagccatg gtcagcaggg
     1801 tgaataaggc aatcatgacc agggagccct tttccacgat cggataataa tccttataaa
     1861 tcaggatggc cgcagacagg ccgacgacca cggtccagat aaaaaccgat cctcccggga
     1921 ataacattct tagcacgatg gctgtacctc ccaccattcc acccaactga acgattttca
     1981 ggaattgaag aaaaaataca gtccataccg cccaggatcc gcggcctatc cggaaaccgg
     2041 gaagaagatt aaaagcccgc acggtggtct ctccggtcca gatggcatgt tttccatatt
     2101 gcaactgcac agccactttg atcaggcaac tcaggatgat gatccagaat gccgcaaaac
     2161 ccgccttggc cccaaggatg gtggtggcga tcaactcccc agagccaaca atggacgcgg
     2221 aaaggataaa acccggccct aaaaatttaa atcttcccca aagagtcgtg ggtggttctt
     2281 tgatgtatgc attcgtaatg aggtatggat ccatgaattc gccggcagac aaaagctaaa
     2341 agtagggatt cgggctctaa taaatttatc ccttactaat actttaatga tcagtaagcc
     2401 acggaccctc cgctttgagt tccctttctc ccttctccgt tctcccttct cctctaaaac
     2461 ccttagctta aaaccacatc caccgatgcc gaaaacggat tgaaagagat gatgctgact
     2521 tccggatggt tttttaataa ggcttccact tcttttttca gaactcccga tccacggcca
     2581 tgtatgatac ggatatgagg acgtttcctt tggatcgatt ccaggatgaa ttgcctgcaa
     2641 atctccagtt gaaactccag gattgacttg cccggagtcg gggtatggtt ttgaataagg
     2701 gcttcgtaat gaaggtccag ttgatctcca aactcccccc ggtcagtctt ccggtcatct
     2761 tctggcagcg gaagctcttc atacccgttc tcctcttcct gatcttcccc gctttcgcgg
     2821 atatccttca agggcgcctc cctgatctcc ccatccggca tcctgatcag gatactaccc
     2881 gggctgccag gcccctggta cctgaccttc aggccggagg aaatgatttc caggggttct
     2941 ccgatccacc aggaagaggg attcaaggtt gaggttgagg ttgaggttga ggttgaggtt
     3001 aaggttaagg ttgaggttga ggttgagaag aggcaaagaa ccct
//
LOCUS       DAOQXT010000211         3044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_34371_length_3044_cov_6.134828, whole genome shotgun sequence.
ACCESSION   DAOQXT010000211 DAOQXT010000000
VERSION     DAOQXT010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34371_length_3044_cov_6.134828"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKM27_19365"
     CDS             complement(<1..726)
                     /locus_tag="PKM27_19365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNR09488.1"
                     /translation="MKLFSRSTGQGPPLVILHGLFGSSDNWQTFAKMLSDHYAVITVD
                     LRNHGLSPHTEEFSFEWIGRDLYQFAEDQGLREFYLMGHSLGGKAAAFFALRYPDRVK
                     KLIILDIATKAYPPHHEKYFEAMLSLDFNLIRNRNEADAWLQRSISSLPVRQFLLKNL
                     VRDAEGRFRWKFNLRGLYEQYDAINVAIESETPFDKPVLVLAGEHSSYIQPEDKEPMR
                     ALFPRASFKTIPGAGHWIHADQPE"
     gene            complement(734..2434)
                     /locus_tag="PKM27_19370"
     CDS             complement(734..2434)
                     /locus_tag="PKM27_19370"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNR09489.1"
                     /translation="MGEILKTGRNPFFYVCLRARTVLWMFLIGAQLSFGQSAPFQSSN
                     LPIFIINTQGNTIVDEPKILADLEIIYNGPGKRNSLTDSSRYFRGSIGVEYRGSTSQT
                     LSPKKPLGFETRKEDGESNLNVSLLGMPEENDWILLAPYSDKSLMRDVLIHHVARQIT
                     PYSSRTAFCELVTNGQYMGVYALMEKIKRDKNRVNIRDLEPKDVSGDKLTGGYILKID
                     KTTGSNPVGGFDSKFRSGNGNNSQSTFYQYEHPEVDVLTDEQKSYIRNLVHAFEASFL
                     QPDYTDPVNGYRKWVDEESMIRFMILNELANNVDGYRLSTFLYKDADNRNKKIHFGPI
                     WDFNLGFGNANYCEGGSSEVLAFEFNNRCPADAWQVPFYWHYLTQDSLYNTNLYKRWS
                     EWRKTILSEQNLMAAIDSMAALLEEAQVRNFQRWNILSQHVWPNAYVGGSYSNEIQYL
                     KNWLSKRLVYLDGAFKQATLPKYYPERYFEPVVFPNPALQGQEVTFKYYIHDYDQIRI
                     EVHDILGRRMFHIEDGSHYSGTNQVKSTSLRLPSGIYVYNIYIEGQKKAFQTGKLLIR
                     "
     gene            complement(2419..>3044)
                     /locus_tag="PKM27_19375"
     CDS             complement(2419..>3044)
                     /locus_tag="PKM27_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR09490.1"
                     /translation="EEDMNYKQAIWRTDPKQSGIAGAMGDIGTHAENLAEYITGLRIE
                     ALCADISIFVPGRLLDDDGNVLLHLDQGAKGILFASQISAGEENNLRIRVYGELGGLD
                     WSQQEPNSLIVKWHGKPMEIYRTAVGDLYPETKAHYRIPAGHPEGYLEAFANIYRNFA
                     YAVIAKKEGKSPDPIYDFPTVQDGVRGMQFIQRVIESGKSNQKWVKF"
BASE COUNT          684 a          775 c          790 g          795 t
ORIGIN      
        1 ctccggttga tccgcatgga tccaatgccc cgccccgggt atggttttaa aagaagcccg
       61 tggaaacaag gcccgcatcg gttctttgtc ttcaggctgg atataggacg aatgttcacc
      121 ggcaaggacg agtaccggtt tgtcaaaggg ggtttcggat tcaatggcta cgttgatggc
      181 atcgtattgt tcgtacaatc ccctcaaatt gaatttccag cggaagcgcc cctcggcatc
      241 ccgcaccagg tttttgagca gaaactggcg gaccggcagg ctggatatgc tcctctgcag
      301 ccaggcatcc gcttcattcc ggttgcggat gaggttaaag tccaggctca gcatggcttc
      361 gaaatatttt tcgtgatgcg ggggataagc ctttgtagcg atatcgagga tgatcagttt
      421 tttcacccgg tcgggatacc ggagtgcaaa aaaggctgcc gccttgcctc ccagggaatg
      481 tcccatcagg taaaattccc ggaggccctg gtcttccgca aactggtata aatcgcggcc
      541 gatccattcg aaactgaact cttccgtgtg cggggacaag ccgtggttgc gcagatctac
      601 ggtgatcacc gcgtagtggt cgctcagcat tttagcaaag gtctgccagt tgtctgaaga
      661 accaaacaaa ccatgcagga tgaccagggg cgggccctgt ccggtggacc ggctgaataa
      721 tttcaccact ttattaacgg attaaaagct tgcccgtctg aaaagccttt ttttgtccct
      781 cgatatagat attatagaca tagatgcctg aaggcagccg caaagaggtg cttttcacct
      841 ggttggtccc gctgtaatgg gatccgtctt cgatatggaa catcctccgt cccaggatgt
      901 cgtgaacctc tatccggatc tggtcgtaat cgtggatata atatttgaag gtgacctcct
      961 ggccctgcag ggccggattg ggaaacacta ccggttcaaa ataacgttcc gggtaatatt
     1021 tgggcagggt cgcctgttta aatgccccgt ccaggtaaac gagcctcttt gaaagccagt
     1081 ttttgaggta ttgtatttca ttggaatatg accctcctac ataggcattc ggccatacgt
     1141 gctgggagag gatattccac cgctgaaaat tgcgaacctg ggcctcctcc aacagggcgg
     1201 ccatcgaatc aatcgccgcc atcaaatttt gctccgataa aatggttttc ctccattcgg
     1261 accagcgttt gtacaggttt gtattgtaca gggaatcctg ggtgaggtag tgccagtaaa
     1321 aagggacttg ccaggcgtcc gccggacacc ggttattgaa ctcaaaggcc aatacttcac
     1381 tggacccgcc ctcgcaatag ttggcgttgc cgaatcccag gttaaaatcc cagatgggcc
     1441 cgaaatgtat ttttttattc ctgttgtcag cgtctttata caaaaatgta ctcagccggt
     1501 atccatccac attattggcc agttcattca atatcatgaa ccggatcatc gattcttcat
     1561 ccacccattt gcggtagccg ttgaccggat cggtgtaatc cggctgaagg aaggaggcct
     1621 caaaagcatg caccaggttg cggatataac tcttttgttc gtcggtcagt acatcgactt
     1681 cgggatgttc atattggtaa aaggtggact gggaattatt cccgtttccg gaacggaatt
     1741 ttgaatcgaa gccgcccacc gggttgctgc ccgtggtctt gtcgatcttc aggatatacc
     1801 cgccggtcag tttgtcaccg ctgacatctt tgggttctag gtcccggata ttgacccggt
     1861 ttttatccct tttaatcttt tccatcaggg catagacacc catatactgg ccattggtca
     1921 ccagttcgca aaaagcggtc cgggaagagt aaggagtgat ttgtctcgcc acatgatgga
     1981 tcaatacgtc gcgcatcagg gatttgtcgc tgtatggcgc cagcagtatc cagtcgtttt
     2041 cttccggcat gcccaggagg ctgacattta gattggattc gccgtcctcc tttcgggtct
     2101 caaaccccag tggttttttc ggggaaaggg tctgcgaagt ggaaccccgg tattccaccc
     2161 caatgctccc acggaaataa cggctggagt cggtaagcga attccgtttg ccgggaccat
     2221 tgtatataat ctccagatcg gccaggatct tgggttcatc gacaatggta ttcccttgag
     2281 tgttgatgat gaaaatggga agattggagg actggaaggg tgcagactgt ccaaaactga
     2341 gttgcgcacc gatcaaaaac atccaaagga cagtcctcgc ccgcaggcag acataaaaaa
     2401 aaggattcct tccggtcttt aaaatttcac ccatttctga ttgctttttc cactctctat
     2461 aacgcgctgg atgaactgca tgcccctcac gccgtcctga accgtgggaa aatcatagat
     2521 gggatccggt gacttgcctt ctttctttgc tattacggcg taggcaaaat tgcggtagat
     2581 attggcgaag gcctcgaggt agccttccgg gtgccccgca ggtatccggt agtgggcttt
     2641 ggtttccggg tacaggtctc caaccgcagt acggtagatt tccatgggtt ttccatgcca
     2701 ttttacaatc aggctgttgg gctcctgctg agaccagtcg aggcccccaa gttccccata
     2761 gacccggatc cggaggttat tttcttcccc tgcggatatc tggctggcaa acaaaatacc
     2821 tttcgcaccc tggtccagat gcaacaatac atttccatcg tcatccagga ggcggccggg
     2881 tacgaagatg ctgatgtcgg cgcacagggc ttcgatccgg agcccggtga tgtattcggc
     2941 caggttttcg gcatgggtcc cgatatcgcc catggcgccc gcgatccctg attgtttggg
     3001 gtcggtacgc cagatggcct gtttataatt catatcctct tcct
//
LOCUS       DAOQXT010000212         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_35445_length_2977_cov_6.460986, whole genome shotgun sequence.
ACCESSION   DAOQXT010000212 DAOQXT010000000
VERSION     DAOQXT010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35445_length_2977_cov_6.460986"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PKM27_19380"
     CDS             complement(<1..886)
                     /locus_tag="PKM27_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNR09491.1"
                     /translation="MSVDKIIPVDIEEQMKSAYIDYSMSVIISRALPDVRDGLKPVHR
                     RVLYAMSDLGLDYNKPFKKTARIVGEVLGKYHPHGDSSVYEAMVRMGQPWSMRYTLVD
                     KQGNYGNIDGDGPAAMRYTEGRLTRLSSEMLDDLDKETVDFRLNFDDSLEEPVVLPSK
                     APNLLINGASGIAVGMATNMPPHNLNEVVAGIIAQIDNPEITIPELMAHIHGPDFPTG
                     GIIYGTEGIREAYETGRGRVVLRGKTDIETVHGHESIIITEIPYQLSKSTLIAKINEL
                     RADDKIEGIHDVRDESARD"
     gene            1086..1793
                     /gene="ubiE"
                     /locus_tag="PKM27_19385"
     CDS             1086..1793
                     /gene="ubiE"
                     /locus_tag="PKM27_19385"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNR09492.1"
                     /translation="MIKPYHSEDSKKEQVKKMFDGIAGTYDFLNRFLSLGIDIAWRKK
                     SLQMLTPYRISHLLDIATGTGDFAIMGASILQPEKITGLDLSANMLKIARQKSPSSRF
                     EFVQGDSERLQFADDSMDAATVAYGVRNFEDLDAGLREIRRVLKPGSPFLVLEFSRVE
                     RFPLKQLFFIYSRYIMPVIGKLFSLDPNAYRYLHESMHAFPSGIDFVKRLESAGFRFI
                     RLKKFSFGICTAYLSEK"
     gene            1766..2473
                     /locus_tag="PKM27_19390"
     CDS             1766..2473
                     /locus_tag="PKM27_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNR09493.1"
                     /translation="MYSLSLRKITGALILLGLSIQLRGQDKYTGNNYRQFAGKNYYFG
                     LSLSFNQSTYKLYRSNYFLNQDSLLVTESLKNPGFSVGIISNVKFGNHFDFRFIPTFS
                     FANRNIRYSDGVAEIDRRIESTFLELPLHVRFTSLPYKDMRVFVVGGIKYAYDISNKS
                     RTKKFASLIKLAPNDFTAEIGAGMQFYFPYFIFSPEIKFSQGIGNILIYNGNNSESSV
                     LEKILSRALTISFNFEG"
     gene            complement(2457..>2977)
                     /locus_tag="PKM27_19395"
     CDS             complement(2457..>2977)
                     /locus_tag="PKM27_19395"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNR09494.1"
                     /translation="LKPSEQRSLPPSVREGIRLHLFIDRTVDEDPDYRESIRMIRATQ
                     GKYAPVVADIYYDYLLHLHWDRYSDHSFPVFRQDAYRTLIRHLPENVNENLAGRVRQM
                     IERDFLESYSSPEHMPGTFSMLKRRVHFENALDRAAGDFERLLPQLIRHFNLVFPRIQ
                     AASEQYRLSLQS"
BASE COUNT          709 a          780 c          718 g          770 t
ORIGIN      
        1 cgtcccgcgc cgattcatcc cggacatcat gaatgccttc tattttatca tccgcccgga
       61 gttcgttgat ctttgcgatc agggtagact tgctcaactg gtatgggatc tcggtgataa
      121 tgatcgattc atgcccatgg accgtttcga tatcggtctt tcccctcagc actaccctgc
      181 cccggccggt ctcataagct tcccggatgc cttcggtacc gtaaatgatc cctccggttg
      241 gaaaatccgg gccatggata tgggccatga gttcggggat ggtgatttcc ggattgtcga
      301 tctgggcaat gatcccggcc acgacctcgt tgaggttgtg cggaggcata ttggtggcca
      361 tgcccacggc tatgcctgaa gcgccgttga tcagcagatt cggggctttg gagggcaata
      421 ccaccggctc ttccagggag tcgtcaaaat tgagcctgaa atcaacggtt tctttgtcca
      481 gatcatcaag catttccgag ctcagccggg taaggcgacc ttcggtgtac cgcatggcgg
      541 caggaccatc cccgtcaata ttgccataat tgccttgttt gtccacgagg gtataccgca
      601 tggaccaggg ctgacccatg cgaaccatgg cttcgtaaac ggacgagtcc ccgtgaggat
      661 ggtatttccc caggacttcg cccacgatgc gcgcggtttt cttaaatggt ttattgtaat
      721 cgaggcccag gtcggacatg gcatataaaa ccctccggtg gaccggtttg agcccatccc
      781 gcacatcagg cagcgccctg gagatgatca ccgacatcga ataatcgatg taggcggatt
      841 tcatctgttc ttcaatatct actggaatga tcttgtctac ggacatatag tattcaaaag
      901 cgtccgcaaa attagctaaa tatggccaga ttttccaaaa aatttagaaa aaaggagtat
      961 tcataattct cacattttca agtaaatata ttttattttt ctttgaggac ggtgggcctg
     1021 gcctctttgg ccggggggac cctgcgtaag aatggtattt ttgggtttca agcggccgtt
     1081 ttagcatgat caaaccctac cacagcgagg attcgaaaaa agaacaggta aaaaaaatgt
     1141 tcgacgggat tgcgggtacc tatgatttcc tgaaccgttt tttatccctg ggcatagaca
     1201 tcgcctggcg caaaaagtcc ctgcagatgc tcacccccta ccggatcagc catctgcttg
     1261 atattgctac gggaacgggt gattttgcca ttatgggggc ctccatcctg caaccggaaa
     1321 agataaccgg cctggacctc tcggccaata tgttgaagat cgcccgccaa aaatcccctt
     1381 cctcccgttt tgaattcgtg cagggcgatt ccgagcggct gcaatttgcc gacgacagca
     1441 tggacgccgc gacggtggcc tacggggtgc gcaattttga agacctggat gccggcttga
     1501 gggagatccg gagggtcctg aaacccgggt cccctttcct ggtcctggaa ttcagccggg
     1561 tggaacgttt tccgctgaaa caattattct tcatttactc tcgttatata atgcccgtga
     1621 tcggaaaact tttttcgctg gacccgaatg cctaccgtta cctgcatgaa tccatgcatg
     1681 cttttccatc cggcattgat tttgtaaaaa gactggaatc cgccgggttc agattcatcc
     1741 ggttaaaaaa attcagtttc ggtatatgta cagcctatct ctcagaaaaa taaccggagc
     1801 actgatcctc cttggcctct ccatccagct gagaggccag gataagtata cgggcaataa
     1861 ttatcgtcag tttgcgggta aaaactatta tttcggactt tccctcagtt ttaaccagtc
     1921 cacttataaa ctataccgtt ccaattattt tctcaaccag gacagcctgc tggtcaccga
     1981 atcccttaaa aatccgggct tctcggtcgg gatcatatcc aatgtgaaat tcggaaatca
     2041 tttcgatttc cggtttattc ccaccttttc ctttgccaac cgcaacatcc ggtacagtga
     2101 tggcgtagcg gaaatagacc gccggataga atccaccttc ctcgaacttc ccctccatgt
     2161 ccggtttacc tccctccctt acaaggacat gcgggtcttc gtggtcggag ggatcaagta
     2221 tgcctatgac atttccaata agtcgaggac aaaaaaattc gcctcgctga tcaaactggc
     2281 ccccaacgat ttcacagcgg aaatcggcgc cgggatgcag ttttattttc cttattttat
     2341 tttttccccg gagatcaagt tttcccaggg gatcggcaat atcctcattt ataacggcaa
     2401 caattcagaa tcttccgtgc tggagaaaat cctctccaga gccctgacca tttccttcaa
     2461 ctttgaaggc tgagccggta ctgctcgctt gccgcctgga tccttggaaa aacgagatta
     2521 aaatggcgga tcaactgagg caataacctt tcaaaatccc cggcggcgcg gtccagggcg
     2581 ttttcaaaat gaacccttcg tttcaacatg gaaaaggtgc ccggcatgtg ctccggggaa
     2641 gaataggatt ccaaaaaatc gcgttcgatc atctgcctga ccctgccggc cagattttca
     2701 ttgacatttt caggcaaatg ccggatcagg gtccggtaag catcctgcct gaacactgga
     2761 aaagaatggt cgctgtaacg atcccagtgc aggtgcagta aataatcgta atagatatcc
     2821 gcgaccaccg gggcatattt gccctgggtg gcccggatca tccggatcga ttcccggtaa
     2881 tccggatcct cgtccaccgt ccggtcgata aacaggtgca gcctgatccc ttcccggacg
     2941 gaaggaggca aggatctctg ttctgaaggt ttgagaa
//
LOCUS       DAOQXT010000213         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_35703_length_2961_cov_5.769443, whole genome shotgun sequence.
ACCESSION   DAOQXT010000213 DAOQXT010000000
VERSION     DAOQXT010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35703_length_2961_cov_5.769443"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKM27_19400"
     CDS             complement(<1..284)
                     /locus_tag="PKM27_19400"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNR09495.1"
                     /translation="MKALKTWDYVYVSVQLVLFILYAFPPFRWTFHLGILLQYLFLAG
                     SFFGLIVLFLAMAQLNTNLTPWPSPKSGGTLVSSGLYRFVRHPIYTGIMV"
     gene            complement(301..930)
                     /locus_tag="PKM27_19405"
     CDS             complement(301..930)
                     /locus_tag="PKM27_19405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNR09496.1"
                     /translation="MSTIPAFDYLFEPALIKEMEKEGRRVHLDAGETLLEIGQPVQTV
                     PIVLAGNVKVSRRDQEGRELLLYYINPMESCAMSINCCVQKVPSEIKATAEEEVDVLY
                     IPIQKMDEWMMKYVTWKNFILKTISIRFNELLRTIDQIAFQKLDERLIRYLKEKSKVT
                     GSTVLNLSHEQIAHELATSRVVISRLLKVLENQQKVLLYRNQIKLLKNL"
     gene            complement(1024..2373)
                     /locus_tag="PKM27_19410"
     CDS             complement(1024..2373)
                     /locus_tag="PKM27_19410"
                     /inference="COORDINATES: protein motif:HMM:NF018583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNR09497.1"
                     /translation="MVTVIIPALNEEEHIGYVVDFARQHPLVSEVLVVDDKSLDNTVA
                     IARAHGARVITSTRLGKGISMREGVLCAVNEILVFLDADIEPYPAGTIDALLAPILSG
                     EADFTKSKFNRNAGRVTELVAKPLMSIFFPELLEFEQPLSGMIAGKKSAFRQIEFNDD
                     YGVDVGILIDLHKLKVPSRQVEIGYIENKSKPWEALGKMSKEVARTILQKAGYPLPSA
                     SEEDPSLLSQIRSQLEFAAEDKLSTLRKLVVFDLDNTLIKGCFMDYFAGAHNLIDQLN
                     QIRRSEKNPIVKAKMIAQLLLGKSIHEIIQVVDHIPIVSGAVEVIAELREKGYMVGII
                     SDGFDFIANHIKNKLGLDFAIANELEFSNSVCTGEVRIPSYFLKGPESVCGHLYCQTN
                     AMLRVLEKFDISKEDALSIGQSRHYSCLLAESGNGPELKARLIKGKSAKGPKDQKEG"
     gene            complement(2588..2836)
                     /locus_tag="PKM27_19415"
     CDS             complement(2588..2836)
                     /locus_tag="PKM27_19415"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNR09498.1"
                     /translation="MRKQIIDVRTPEEFSAGHVKGSINIPLQDIPYQVQKIKTIKQPL
                     VLCCASGNRSGQAAVFLKNQGIDCENGGSWLDLRYQMN"
BASE COUNT          715 a          673 c          788 g          785 t
ORIGIN      
        1 aataccatga tcccagtgta gatcgggtgg cgtacgaaac ggtacaagcc agaacttacc
       61 agggtgcccc cggatttggg acttggccag ggggtgaggt tggtattaag ctgggccatc
      121 gcgaggaaga ggacgatcag cccgaaaaag gatcccgcaa gaaagagata ttgtaaaagg
      181 atccccaggt ggaaggtcca ccggaagggg ggaaatgcat aaaggataaa caggacaagt
      241 tgaacggata catacacata gtcccaggtt ttgagcgctt tcatatttgc ggacaggggc
      301 ttagaggttt ttgagcaact tgatctggtt gcggtacagc aatacttttt gttggttttc
      361 caacaccttg agcaagcggg agatcaccac cctcgaagtg gcgagttcat gggcaatttg
      421 ttcgtgggaa agattgagta cggtggaccc ggtgaccttt gatttttcct tcaggtaacg
      481 gattagcctt tcatccagtt tctggaaggc gatctggtca atggtccgga gcagttcgtt
      541 gaaacggatg ctgatggttt tgaggataaa atttttccag gtgacatact tcatcatcca
      601 ctcgtccatt ttctggatcg ggatgtaaag gacatccact tcctcctccg ccgtggcctt
      661 gatttcactg ggaacttttt gcacgcaaca attgatggac atcgcgcaac tttccatggg
      721 gttgatgtaa taaagcaaga gttccctgcc ttcctggtcc ctccggctca ccttgacatt
      781 gcctgcaagg acaatgggga ccgtctgcac cggttgcccg atttcaagca gggtttctcc
      841 ggcatccagg tgaacgcggc gcccttcttt ttccatttct ttaatcaggg cgggttcaaa
      901 caaataatcg aaggccggaa tggtactcat ggatccgaaa attacactta aagttcagaa
      961 cgcgtgaatc cggaaggtat aaatccggag gtgggtccat ttgacccgtc gcagcaaggg
     1021 acattacccc tccttttgat cctttggccc ttttgcggat ttccctttga tgagccgggc
     1081 cttgagttcc ggtccattcc cggattcggc aaggaggcag gaatagtgcc tggactgacc
     1141 gatcgagagg gcgtcctcct tgctgatgtc gaatttttcc aggacccgga gcatggcatt
     1201 ggtctggcaa tacaggtgcc cgcaaacact ttccgggcct ttcaggaaat aggaaggaat
     1261 cctgacttcc ccggtacaaa cactgttcga gaattccagt tcgttggcga tggcgaagtc
     1321 caggcccagc ttgtttttaa tgtggttggc aataaaatca aatccatccg agatgatgcc
     1381 gaccatgtaa cccttttccc tgagttcggc aatcacttcc acggcgccgg agacaatggg
     1441 gatgtggtcg accacctgga tgatttcatg gatcgatttt ccaagcaaca attgggcgat
     1501 catttttgct tttacgattg ggtttttttc tgaacgcctg atttggttca gttgatcgat
     1561 gagattatga gcgcctgcga aataatccat aaaacaccct ttgatcaggg tattgtccag
     1621 gtcaaagacg accagtttcc gcagggtgga taatttgtcc tctgcggcaa attcaagctg
     1681 ggaccggatc tgactcagca gagacgggtc ttcttcggat gcagacggaa gagggtatcc
     1741 ggctttctgc aaaatggtgc gcgccacttc cttgctcatt tttccaaggg cctcccaggg
     1801 tttgcttttg ttttcgatat aaccgatttc cacctggcgg ctggggactt ttagtttatg
     1861 caggtcgatc aggatgccga catcgacccc gtaatcatcg ttgaattcta tttgtcgaaa
     1921 ggctgatttt tttcctgcga tcatcccgct gaggggttgt tcaaactcga ggagttccgg
     1981 gaaaaaaata ctcatcaggg gtttggcgac cagttcggtc acccggcctg cgttccggtt
     2041 gaatttggat ttggtgaaat ccgcttctcc tgacaggatg ggagccagga gggcatcgat
     2101 ggtcccggct ggataaggtt cgatatccgc gtcgaggaaa accaggattt cattcacggc
     2161 gcaaaggaca ccttcgcgca tggaaatccc ttttccaagc cgggtgctgg tgatcacccg
     2221 ggctccgtgg gcacgggcaa tggctaccgt attatccagg gatttgtcgt ccacgacgag
     2281 cacttcactg accaggggat gttgcctggc gaaatcgacg acgtatccta tgtgttcttc
     2341 ttcatttaag gcaggtataa tgacggtgac cataatccaa agcgtttaaa tgatgagata
     2401 ataaaagaac atcccggtct ttccttgacc ggtaatcaaa ttaacaggat tatcacgaag
     2461 gattcaaggg gtatagtatc tgagggagaa ttttaatggt gggtggtggg tggtgggtta
     2521 gtgggcggaa ataaaaaagc cggaagcaag ggccaccggc ttcaaaatct ggttaggatc
     2581 ttgactatta attcatctgg taacgaaggt ccagccagct gcctccattt tcacaatcga
     2641 ttccctgatt tttcagaaaa accgctgcct ggccgctccg gtttccactg gcgcagcaaa
     2701 gtaccaatgg ttgtttgatg gttttgattt tttggacctg atacggaata tcctgcaagg
     2761 ggatattgat gcttcccttc acatggcctg cggaaaattc ctcgggagtc ctcacgtcga
     2821 tgatctgttt tctcatttat tatgtttttt tgaagccaca aaagtggtag gagaaaacat
     2881 gccgtgtcag taactaatgt tacagtagtc aagagtcaag agtcaagagt caagagtcaa
     2941 gagtcaagag tcaagagtca a
//
LOCUS       DAOQXT010000214         2890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_37045_length_2890_cov_5.087478, whole genome shotgun sequence.
ACCESSION   DAOQXT010000214 DAOQXT010000000
VERSION     DAOQXT010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37045_length_2890_cov_5.087478"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1676
                     /locus_tag="PKM27_19420"
     CDS             177..1676
                     /locus_tag="PKM27_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR09499.1"
                     /translation="MTALVIFGIIALLAIVVVQIGKLSDLRSKIMGEEDIQYQNNDIT
                     GKLLVLFMVGFLIFCVVSALYYKNWMLGYGPLKSASEHGGSIDGLFNTTLFFTGIVFV
                     VTQVLLFWYAYKYRSQRGRKAIYFPGSTKLELVWSIVPSIVLVILVANGLIAWNDIMA
                     DTQPEDGALEIEATGYQFAWNIRYPGPDGELGAKNYKKITGLNGLGQDWTDIKNLDDF
                     NADEIVLPVGKKVRVRITSKDVLHNFYLPHFRVKMDAVPGLPTHFVFTPIKTTEEFRE
                     GLRQYKEYQAPSDPNDPASEPLWKTFNYELGCAELCGIGHYSMRRLVRIVSEQEYESW
                     LASQKSTYMTSIRNSDEDPFKGQLLDVEVKARRDAFFSEVDKIIKDDNLIGKEALRLE
                     NIQFETGSDKLTAFSKYELDNLIELMVNNSTMTIELSGHTDNTGDAAANLDLSNRRAA
                     AVRDYLVSRGIQAERMKSVGYGQTRPVEPNDTEAGRQKNRRTELRILSK"
     gene            1699..>2890
                     /locus_tag="PKM27_19425"
     CDS             1699..>2890
                     /locus_tag="PKM27_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNR09500.1"
                     /translation="MAEVLENKVNLPDVDQLIREQGYDDHFHEHHHGDKYQSGFISKY
                     IFSMDHKIIARQFLITGMIWAVIGGLMSLIFRLQLGFPEENFAWLRPLIGRWVTVNDA
                     GVGTITPEFYYAMVTMHGTIIVFFVLTAGLSGTFSNLLIPLQVGARDMASPFLNMLSY
                     WFFFLSSIVMLLSLFLSTGPFSGGWTAYPPLSALPQASDGSGAGMTMWLVSLTLFVVS
                     VLLGGINYITTVLNLRTKGMSMWKLPLPIWAFFTTAVLGLLSFPVLVSGFLLLFFDRS
                     LGTSFYLSEIFVGGQALDFAGGSPILYQHLFWFLGHPEVYIIILPAMGIVSEVLSVNS
                     RRPIFGYRFMVYSILAIGFLSFIVWAHHMFMSGVNPFISNFFVIFTLIIAVPSAVKVF
                     NWLTT"
BASE COUNT          665 a          762 c          717 g          746 t
ORIGIN      
        1 ggatttacac ttcccggcct gctggatctt ggggtgatga ttggattcct ggcgggtttt
       61 cttttgttcg tattccggga aatggggaaa gccccgctgg tacctaaaaa cgatccttat
      121 tattcagaaa gtttgcatca ccatgtgatg tgatcagata aaatgtaaaa aatattatga
      181 ccgctttagt catttttgga atcattgccc tgttggccat cgtcgtggta cagatcggga
      241 agttgagtga tctccgttcc aagatcatgg gggaagaaga catccagtat caaaacaatg
      301 atatcaccgg taaacttttg gtcctgttta tggtgggatt cctcattttc tgcgtggtgt
      361 cggcccttta ttataaaaac tggatgctgg gttatggccc cctcaaatcg gcgtccgaac
      421 acggaggcag catcgatggc ttgttcaata ccaccttgtt tttcaccggg atcgtatttg
      481 tcgtcaccca ggtccttctg ttctggtatg cctataagta ccggagccag cgcggccgca
      541 aggcgatcta tttccccggc agcaccaaac tcgaactggt ctggtccatc gtgccctcca
      601 tcgttctggt catcctggtc gccaacggac tcatcgcctg gaacgatatc atggcggata
      661 cccaacctga agacggtgca cttgaaatcg aagccaccgg ataccagttt gcctggaata
      721 tccgctatcc cggcccggat ggcgagctgg gcgccaaaaa ttataaaaag atcaccgggc
      781 tcaacggatt ggggcaggac tggacggaca ttaaaaacct ggatgatttt aatgccgacg
      841 aaattgtatt gccggtagga aaaaaagtgc gcgtccggat tacttccaaa gatgtcctgc
      901 ataacttcta tttgcctcat ttccgggtca aaatggatgc cgtgcccggc cttcccaccc
      961 attttgtatt caccccgata aagacaacgg aggaattccg ggaaggcctc cggcagtata
     1021 aggaatacca ggccccctcg gaccccaacg acccagccag cgaaccgctc tggaaaacct
     1081 tcaattacga gctcggctgc gctgaattgt gcggcatcgg ccactattcc atgaggaggc
     1141 tggtgcgcat cgtttccgaa caggaatacg agtcctggct cgccagtcag aaatccacct
     1201 acatgacttc catccgcaac agcgatgagg atcctttcaa agggcaattg ctggatgttg
     1261 aagtcaaagc ccggagggat gcctttttca gtgaagtgga taaaatcatc aaggatgaca
     1321 acctgatcgg caaggaagcc cttcgcctgg agaacatcca gtttgaaacg ggcagcgata
     1381 aactgaccgc attttccaaa tatgaactgg acaaccttat tgagctcatg gtcaacaatt
     1441 caaccatgac cattgaactc agcggtcata ccgacaatac cggcgatgcc gccgccaacc
     1501 tcgatctctc caaccggcgc gccgccgcgg tcagggatta cctggtcagc cgcgggattc
     1561 aggctgaacg catgaaatcc gtcggttacg ggcaaacccg tccggtggaa cccaacgata
     1621 cggaagccgg aaggcaaaaa aaccgcagga ccgaattgcg aattttatct aaataacaaa
     1681 aaataaaact tcaacccaat ggcagaagta ttggaaaata aagtaaatct cccggatgta
     1741 gaccagttga tccgggagca gggttatgat gatcattttc atgaacatca tcatggggac
     1801 aaataccagt ccggcttcat cagcaagtat attttcagca tggaccataa gatcatcgcc
     1861 cgtcaattcc tgatcacggg tatgatctgg gcggtcatcg ggggcctgat gtccctgatc
     1921 ttccggcttc agctgggctt ccccgaggaa aattttgcct ggctccggcc actgattggc
     1981 cggtgggtga ccgtcaacga tgccggggtg ggtaccataa ctcctgaatt ctattacgcc
     2041 atggtcacca tgcatggaac gatcatcgtt ttcttcgtat tgaccgcggg gctcagcggc
     2101 accttcagca acctgttgat tcccttgcag gttggcgccc gggacatggc ttccccgttt
     2161 ttaaatatgc tttcctactg gtttttcttc ctgtccagca tcgtgatgct tttatccctt
     2221 ttcctgagta ccgggccctt ttccggcgga tggaccgctt atcctccgct cagcgccttg
     2281 ccgcaggctt cggacgggtc gggcgcgggt atgacgatgt ggctggtgag cctgacctta
     2341 tttgtggtat cggtcctgct gggcgggatc aattacatca ccaccgtgct taaccttcgc
     2401 acgaaaggaa tgagcatgtg gaaactgcct cttccgatct gggcattttt tactacagca
     2461 gtcctcggcc ttttatcgtt cccggtattg gtatccggat tcctgctcct gttttttgac
     2521 cgcagcctcg ggaccagttt ctacctcagc gaaatttttg tcggagggca ggcccttgat
     2581 tttgcggggg ggagcccgat cctttaccag cacctgtttt ggttcctggg acatccggag
     2641 gtttatatca tcatcctgcc cgctatgggt atcgtttcag aagtactgtc cgtaaacagc
     2701 agacgaccga ttttcggata tcgttttatg gtatactcca tcctggccat tggttttctg
     2761 tccttcatcg tatgggccca tcacatgttt atgtccgggg tcaacccatt tatctccaat
     2821 ttctttgtca tattcaccct catcattgca gtcccctcgg cggtaaaggt cttcaactgg
     2881 ctgactacgc
//
LOCUS       DAOQXT010000215         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_37485_length_2869_cov_6.365672, whole genome shotgun sequence.
ACCESSION   DAOQXT010000215 DAOQXT010000000
VERSION     DAOQXT010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37485_length_2869_cov_6.365672"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /gene="groEL"
                     /locus_tag="PKM27_19430"
     CDS             <1..778
                     /gene="groEL"
                     /locus_tag="PKM27_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNR09501.1"
                     /translation="AMLEDIAILTGGTVISEEKGYKIENVELDLLGQAEKITVDKDNT
                     TIVNGRGDTKMIQARIGQIKQQIETTTSDYDKEKLQERLAKLSGGVAVLYVGAATEVE
                     MKEKKDRVDDALHATRAAIEEGIVPGGGVALVRGIPAMDKMKGANEDETIGIQIVKKS
                     LEAPLRIIAENAGVEGSVVFQKVLSSKGALGYNARTDVYEDLKKAGVIDPTKVTRIAL
                     ENAASIAGMILTTECVVNEKPKDKEPGHAHPPGGGMDGMM"
     gene            946..>2869
                     /gene="pbpC"
                     /locus_tag="PKM27_19435"
     CDS             946..>2869
                     /gene="pbpC"
                     /locus_tag="PKM27_19435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNR09502.1"
                     /translation="MLKGLRIKRTLVIGLVILVAGLALGLWTIPFPSQGFSLALFSAD
                     GRLLGARLSDDQQWRFNPGNALPEKYIQCLLTYEDRYFYRHPGINPVSLIKALERNLR
                     AGKVVSGGSTLTMQDIRMRRGPRSRNLWNKSLEMFWAISLELKWSKKKILEDYASRAP
                     FGSNVVGLEAACWRYFDKSPNQLSWAEAACLAVLPNNPSMIHPGRNRSSLQSKRDRLL
                     ESLLRIRILDSLTYRLALMEPVPMAPRPLPDLAPHALDYLAKRGPSNPRLTTTLDYSI
                     QQMAREIAKWGHDRLSAQYIQNIALLIAETRTGKVLAYCGNAPNTALSPFVDHIQAPR
                     STGSILKPFLFASALYQGTLLPGTLMDDIPVVIDGFQPQNFSGHYTGMIPMDQALIRS
                     LNIPFVHLLKAYGLESFHTKLRQLGQRHILRPPSHYGLSLILGGAESSLWDLSGAYAS
                     MARVLQHYHEADRRYFSEDIHPLRLLEVENEEPRSTGVKDPVLLSAASIYQTLEVLKT
                     LNRPDESGQWASFKQSRDIAWKTGTSYGFRDAWAIGLDPDYTVGVWVGNADGEGRPGL
                     LGIKAAAPLLFDLFSRLPRSGQSWFEPPLDELTPVTVCRSSGMKATGICPATDTLLMD
                     RHHDLTPLCNLHTSIWI"
BASE COUNT          682 a          805 c          761 g          621 t
ORIGIN      
        1 agctatgctg gaagatattg ccatcctcac cggaggaacg gtgatctccg aagaaaaagg
       61 ctataaaatc gaaaatgtgg aactggacct gcttggccag gctgaaaaaa tcaccgtgga
      121 caaagacaat accactattg tcaacggccg gggcgacacc aagatgatcc aggcccggat
      181 cggccagatc aaacagcaaa ttgaaaccac cacctccgat tacgacaagg aaaaattgca
      241 ggaaaggctg gctaaattgt ccggtggtgt tgccgttctg tacgtaggcg ccgccacgga
      301 agtggaaatg aaggagaaaa aagaccgcgt ggacgatgct ttacatgcca cccgtgccgc
      361 tattgaagaa ggtattgtac ccgggggagg cgttgcgttg gtgagaggca tacccgctat
      421 ggataaaatg aaaggagcca atgaagatga aaccatcgga atccagatcg taaaaaaatc
      481 cctcgaagcc cccttgcgca tcatcgccga aaacgccggg gtagaaggca gcgtggtgtt
      541 ccaaaaggta ctcagcagca aaggcgccct gggttacaat gcccggacgg acgtctatga
      601 agacctgaaa aaagccgggg tcattgatcc tactaaagta acccgcatcg ctctggaaaa
      661 tgcggcttcc atcgcaggga tgatcctgac caccgagtgc gtagtcaacg aaaaaccgaa
      721 agacaaggaa ccgggccatg cccatcctcc cggcggaggc atggatggca tgatgtaatc
      781 ggcacctctt ttccgtttaa aaataaaagg cagtcgttcc ggctgccttt tttatttgcc
      841 ttcgtggatt accgggaatc ccgtaagccc cattcaccgg tttccagtcc ggatttcaag
      901 attagtttgg tggcggggcc tgaaagcaaa taaatttggg atttcatgct gaaaggcctt
      961 cggataaaaa gaaccttagt catcggcctg gtgatcctgg tcgccggtct tgccctgggc
     1021 ctgtggacca tccccttccc ttcccagggg tttagcctgg ccctgttttc cgccgatggg
     1081 cgccttctgg gagcccggct ttccgatgat cagcaatgga ggttcaatcc cggcaatgcg
     1141 cttccggaaa aatatatcca atgcctgctt acttatgaag accggtattt ttacaggcat
     1201 ccgggcatca acccggtcag tttaatcaaa gccctggagc gaaacctccg ggccgggaag
     1261 gtcgtcagcg gcgggagtac cctcaccatg caggatatcc ggatgcggcg gggtcccagg
     1321 agcaggaatc tttggaacaa gagcctggaa atgttctggg cgatctccct cgaattaaaa
     1381 tggtccaaaa aaaagatact ggaagattat gcctccaggg cccctttcgg ttccaatgta
     1441 gttgggctgg aagccgcttg ctggagatat tttgacaaat caccgaacca gctttcctgg
     1501 gcggaagcag cctgcctggc cgtcctgccc aacaaccctt ccatgatcca tccgggcaga
     1561 aatcggtcct cgctccagtc aaagcgggat cggctcctgg aaagcctcct caggatccgt
     1621 atactggatt ccctgactta ccggctggcc ttgatggaac cggtgcccat ggccccccgc
     1681 ccgctgccgg acctggctcc ccacgccctg gattatctcg ccaaacgggg accttccaac
     1741 ccacggttga caacgaccct ggactattcc attcaacaga tggccaggga aattgccaag
     1801 tggggacacg accggctgtc cgcccaatac attcaaaata ttgccttgct gatagcggaa
     1861 acccgaaccg gaaaagtgct ggcctattgt ggaaatgccc cgaataccgc cctcagccct
     1921 tttgtggacc atatccaggc tccccggagt acgggaagca tcttaaaacc attcttgttc
     1981 gcctcggccc tctaccaggg taccctcctg cccggaaccc tgatggatga catccccgtg
     2041 gtcatcgatg gatttcagcc ccagaatttt tctggtcatt acaccggcat gatccccatg
     2101 gaccaggcgc tgatccggtc cctgaacatc ccttttgtcc atttgcttaa agcctacggc
     2161 ctggagtcct ttcataccaa attgaggcag cttggacaaa ggcatatcct ccggccgcct
     2221 tcgcattacg ggctcagcct gatcctggga ggggccgaat cctccctctg ggacctttca
     2281 ggagcttatg catccatggc ccgtgtcctt cagcattacc acgaagcgga tcgcaggtat
     2341 ttttccgaag atatccatcc attgcgcctg ctggaggtgg aaaatgagga accccgttca
     2401 acaggagtga aggatccggt ccttttgagt gcagccagta tttaccagac cctggaggtc
     2461 cttaagaccc tgaaccgccc cgatgaatcc gggcaatggg cttcctttaa acaatcccgc
     2521 gacattgcct ggaagaccgg taccagctat ggttttcgtg acgcctgggc tatagggctc
     2581 gacccggact ataccgtggg agtctgggtg ggcaatgcgg acggggaagg ccggccgggc
     2641 ctgctgggca tcaaggcagc agcaccgctt ttattcgatc ttttttcgag attgccacga
     2701 agcgggcagt cctggtttga accgccgctg gatgaactga ctccggtgac ggtttgccgg
     2761 tccagcggta tgaaagctac cggtatttgc ccggccaccg ataccctgct gatggaccgg
     2821 catcatgacc tcactccttt atgtaatttg catacctcca tctggatca
//
LOCUS       DAOQXT010000216         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_37500_length_2868_cov_5.168859, whole genome shotgun sequence.
ACCESSION   DAOQXT010000216 DAOQXT010000000
VERSION     DAOQXT010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37500_length_2868_cov_5.168859"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1312
                     /locus_tag="PKM27_19440"
     CDS             98..1312
                     /locus_tag="PKM27_19440"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR09503.1"
                     /translation="MTKRRDVLKGAMGLPLLGTILPGLQQKPEEQIGDFERDYFKELG
                     LRTFINAAGTYTALTASLMHPEVIKAINYARLQFVRLEDLQDKVGERLSELLHCEGAM
                     VTAGAASAITLGTAAIVTGGDQDNIERMPVDMTGMKTEVISQKTHRVGYDHAMRNCGL
                     KVVEVETVKELEAAINEKTAMMWFLNYSNFAGKIRDEEYLAIAKKHNIPTLIDCAADV
                     PPVEMLWKHTKMGYDLVCFSGGKGLRGPQSAGLLLGRKNLIQAARKNAPPNGNTIGRG
                     MKVNKEEILGMLAAIEVYLSRDHKKDWALWESQVDLIAKAASSIPGVTPEKHVPEIAN
                     HVPSLRIRIDPSRVKISPEQLRLALRNGHPSIETMGGSETVDITTWMMIPGEERIVAK
                     RVKDILLQASVE"
     gene            1323..2051
                     /locus_tag="PKM27_19445"
     CDS             1323..2051
                     /locus_tag="PKM27_19445"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNR09504.1"
                     /translation="MAEWLADQSGISRILAALILFLPMLSNGQTRSPYEVHRIPAGKM
                     TLTGRGDHPLWRKAGPLTDFIYPWNEGRPPAMSFQAVHDKDWLYGLFRVHDPKKILVY
                     RDKDSKLETLRSDRVEIFFRQDARMDPYYGLEMDAEGRVYDFQTSFPNKSNPAWFWPA
                     GHLKIASAYTSEGYTLEFAVSKKSLKELGLLKKNKIEAGLYRGECISIEEGKAEFKWI
                     SWVRPDTETPQFHTPSSFGLLMLR"
     gene            complement(2204..>2868)
                     /locus_tag="PKM27_19450"
     CDS             complement(2204..>2868)
                     /locus_tag="PKM27_19450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR09505.1"
                     /translation="KNPLTPMKLITQHLQNSMSRAGQEEIPNLVKRVTQTLIEQIENL
                     SKIASEFSNFAKLPAPENEKLVMNEVVTSTHDLFRKREDMDIHLRVPIDEIYIFADKN
                     HVIRVLTNLIKNAIQAIPVGRRGKIEIVLYKQDEKAIVQVTDNGCGIPESMKDKVFYP
                     NFTTKNSGTGLGLAIVRDIVDCCNGAIYFTTAENKGTSFFVEFPLMHMEDNFKVVERV
                     SL"
BASE COUNT          710 a          721 c          766 g          671 t
ORIGIN      
        1 cccccgtccg caccaccatc gccgcggcgg gaggcatacc gggcaattcc ctggtggaaa
       61 tcgattgtat tgcatatata gattaaaatc aaggaccatg accaaaagaa gagacgtcct
      121 caaaggcgcc atgggattgc ccttactggg gaccatcctg ccaggcttgc agcaaaaacc
      181 ggaagagcaa attggtgatt tcgaaaggga ctattttaag gaactggggc ttcgcacctt
      241 tatcaatgcc gccggtacct atacggccct gacggcatcc ctgatgcatc ccgaggtgat
      301 caaagccatc aattacgccc ggctgcaatt tgtccggctg gaagacctcc aggacaaggt
      361 tggggagcgg ctttccgaat tgcttcattg cgaaggggcc atggtgaccg cgggtgcggc
      421 atcggccatc accctgggga ccgccgccat cgtgaccggg ggcgaccagg ataatatcga
      481 gcgcatgccg gtggatatga cgggcatgaa gaccgaagtg atcagccaga agacccaccg
      541 ggtgggatat gatcatgcga tgcgcaactg cgggctgaaa gtggttgaag tggaaaccgt
      601 gaaagaactg gaagcggcca tcaatgaaaa aacggcgatg atgtggttcc tgaattacag
      661 caattttgcc ggtaaaatca gggatgagga gtatctggct attgccaaaa aacataatat
      721 tccgacgctc attgattgcg cggcggatgt gcccccggtg gaaatgttgt ggaagcatac
      781 caagatgggt tacgacctgg tttgtttttc cggaggaaaa ggcctgagag ggccgcaaag
      841 cgccggcctc ttgcttggcc gtaaaaacct gatccaggct gcccgtaaaa acgccccgcc
      901 gaacgggaat accattggcc ggggcatgaa agtcaacaaa gaagagattc tcggaatgct
      961 ggctgccatt gaagtctacc tgtcccgtga tcataaaaaa gactgggcgc tgtgggaaag
     1021 ccaggtcgac ctgatcgcaa aggccgcgtc ttccattccg ggggtgactc ctgaaaagca
     1081 tgtgcctgaa atagccaatc acgttccctc cctgcgcatc cgcatcgacc catccagggt
     1141 aaaaatcagc cccgagcaat tgcgcctggc cctgcgtaac gggcatcctt cgattgaaac
     1201 catgggaggt tccgaaaccg tcgacatcac cacctggatg atgattcctg gagaagaaag
     1261 aattgtagct aagcgggtga aggatatcct cctgcaggct tccgttgaat aaattccggt
     1321 gaatggctga atggcttgca gaccagtccg ggatttcccg aatcctggcc gctttgatcc
     1381 tattcctacc catgctttca aacggacaaa ccaggtcacc ttatgaagtc caccggatcc
     1441 cggcgggcaa aatgaccctg acgggcaggg gcgaccatcc cttatggagg aaagccgggc
     1501 cgctgactga ttttatttat ccctggaatg aaggacgacc tccggcaatg tcctttcagg
     1561 ctgtgcatga taaggattgg ctgtacggcc tgttccgggt gcacgatcca aaaaagatcc
     1621 tggtttaccg cgataaggat tcaaaactgg aaaccctccg gtctgaccgc gtggagatct
     1681 ttttcaggca ggatgcgagg atggatccgt attatggcct ggaaatggat gccgaaggcc
     1741 gggtatatga tttccagacc agttttccca ataagtccaa cccggcctgg ttttggccgg
     1801 cgggacattt gaaaatcgct tcggcctata cttctgaagg ctataccctt gaattcgcgg
     1861 tcagcaaaaa atcactgaaa gagctgggtc tcctgaagaa aaataaaatc gaggcgggat
     1921 tgtaccgggg cgagtgtatc agcatcgagg aggggaaagc cgaatttaaa tggatcagtt
     1981 gggtccgccc ggatacggaa acccctcaat ttcatacgcc ttcatcattc ggtttgctga
     2041 tgcttcgcta aatcctcttt tgctcccttt gccattgacc gtttaccttt cgccataagg
     2101 agaaaatgca aatctcgtcc cgtccgggac gaatgccccc cttgcgaatg atgctaccca
     2161 tattgaatcc ctgacgggat ccaatgaaaa accatttgtt ctgctataaa ctcacccttt
     2221 ccactacctt aaaattgtcc tccatatgca tgagagggaa ctccacaaaa aaggaagtgc
     2281 ctttattttc ggccgtggta aaatagatgg caccgttgca acaatccacg atgtccctca
     2341 cgatcgcaag tcccaggccg gtacctgagt ttttggtggt aaaattggga taaaagacct
     2401 tgtctttcat ggattcgggg atcccacaac cgttgtccgt gacctggaca atggcttttt
     2461 catcctgctt gtacaagaca atttcgatct tgcccctgcg gcctacaggt atcgcctgga
     2521 tggcattttt gatcaggttg gtcaggaccc ggatcacatg gtttttatcg gcgaaaatat
     2581 aaatttcatc aatcggcacc cggagatgga tatccatgtc ttcccgtttc cggaacaggt
     2641 catgggtgga ggtgaccact tcattcatca ccagtttttc attttcagga gcgggtagtt
     2701 tggcaaaatt ggaaaactcc gaagcgatct tggacaagtt ttcgatctgt tcgatcaggg
     2761 tctgggtgac tctttttacg agattcggga tctcctcctg cccggccctg ctcatggaat
     2821 tctggaggtg ctgggtgatc agtttcatgg gggtcagcgg gtttttga
//
LOCUS       DAOQXT010000217         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_37523_length_2866_cov_5.281750, whole genome shotgun sequence.
ACCESSION   DAOQXT010000217 DAOQXT010000000
VERSION     DAOQXT010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37523_length_2866_cov_5.281750"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKM27_19455"
     CDS             complement(<1..619)
                     /locus_tag="PKM27_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HNR09506.1"
                     /translation="MEKKFSYEEINPLNSIEEWEEDVLVRYPDPEKIATSKATEAYRN
                     YETPVRDTVREFYRLNHTYQTYDFVVGKKNDFLKFDKKEMPVWDAFQFLNQLVDDSDP
                     DTDLDQLQHLLQTSEAIRRDGHPDWMVLTGLMHDMGKVLCLFGEPQWAVVGDTFPVGC
                     AYSDKVVYPEYFSLNPDYTNNNYNSTLGVYEKGCGLRNVHMSWGHD"
     gene            771..1802
                     /locus_tag="PKM27_19460"
     CDS             771..1802
                     /locus_tag="PKM27_19460"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR09507.1"
                     /translation="MALLTIKQLAEQLGLAPSTVSKALRDSYEISAETKQRVLEAARQ
                     YHFTPNPYASSLRRKNSRTIAIVVPEVEDSFFSTAINGIEFVAHSADYHVLIYLTHED
                     QALEESILRELSNGRVDGILISVATGSASARHLLDYNMSGKPLVFFDRVLDEVPVHKV
                     ITDDFEAGYLASKHLLENGCTHISFLSIGDGLNIMKQRFDGGAKAVREFRPGTPYHYV
                     NCSPDPNTARILIQKLLTSANQPDGIIGAVEKLAIATYLECKSLEIRIPEDLRVLAFS
                     NLPTASLLKPGLSTIRQPAYEIGKKAASLLFESLNKNKPPFTPKSIVVPSVLEPRAST
                     LWKEWQDHQ"
     gene            complement(1796..>2866)
                     /locus_tag="PKM27_19465"
     CDS             complement(1796..>2866)
                     /locus_tag="PKM27_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNR09508.1"
                     /translation="WGLLARLYLNGAVYRAPYSATFAFPQEDMDKVIEYCDKIIGSNL
                     YTLSRDYFSIFNDENHTNKELIFAVDQRADLNGHNRLAYFSLSGDQFPLPAFPAANGT
                     DGPGITSDFYQTWAQAYAPADPSVDPRFYQQNLSIYSNPADSCVDGASFRINRGILRG
                     QQYGLIRRNGVFLKCADGKFKVGPLFHDTRNKPTLAVDFTERIDFTVEGSNYNTGYRV
                     EKYEFSKKSASGRNFGEADIVIVRLADIYLMRAEAKLRKSGDAASALADVNTVRASRN
                     LTTPPPALTSMSLDLLYRERGFEFYWEMLRRTDMIRFGKYEGTWTEKDNTDKNKRVFP
                     IPQTAIDGASNIPGYLVQNPGY"
BASE COUNT          678 a          782 c          728 g          678 t
ORIGIN      
        1 cgtcatgacc ccaggacatg tgtacattgc ggagcccgca gcctttttca taaacgccga
       61 gggtggagtt gtagttgttg ttggtataat ccgggttgag agagaaatat tcgggataaa
      121 ccaccttgtc cgaataagcg cagccgaccg ggaaagtatc cccgactacc gcccactggg
      181 gctcgccaaa aaggcaaagc accttgccca tgtcgtgcat cagcccggtg agcaccatcc
      241 agtccggatg gccgtcccgg cgaatggctt ccgaggtctg aagcaaatgc tgcaactggt
      301 cgaggtcggt atccggatcg gaatcgtcca ccagttggtt gagaaactgg aaagcatccc
      361 ataccggcat ttcctttttg tcaaatttca ggaaatcatt ttttttgccc accacaaaat
      421 cataagtctg ataagtatgg ttcaagcggt aaaactcccg tacggtatcg cgaaccgggg
      481 tttcataatt acggtaggct tccgtcgctt ttgaggtagc gattttttcc gggtcgggat
      541 aacgcaccag cacgtcttct tcccattctt caatgctgtt caggggattg atttcttcgt
      601 agctgaattt tttttccata acctaaatgc ttgattgcca aaaataaggt ttcccggggg
      661 aaatattcag tccggcttcc cgggcaacac aggttcacag gtcaatctgc gaaatcgttt
      721 tcgattaaac cgattttggt tcgtaatctt gcaatctccg aagccccaaa atggccctcc
      781 tcacgataaa acaactggct gagcagcttg gtcttgctcc ctcgaccgtc tccaaagcgc
      841 tgagagacag ctacgagatc agcgccgaga ccaaacaaag ggttctggaa gctgcccggc
      901 agtaccattt tacacccaat ccctatgcca gctcccttcg ccggaaaaac agccggacca
      961 tagccatcgt cgttcccgaa gtggaagaca gctttttttc aacggccatc aacggcatcg
     1021 aatttgtcgc ccattcggcg gattaccatg tgctgatcta ccttacccat gaagaccagg
     1081 cgctggagga atccattctc cgggaattgt ccaacggcag ggtggatggc atcctgattt
     1141 ccgtcgccac gggcagtgcc tccgcccggc atctcctgga ctacaacatg tccgggaaac
     1201 cgctggtctt ttttgaccgc gtattggatg aggtcccggt ccacaaggtc attaccgatg
     1261 attttgaggc gggctatctg gcttcgaagc atctgctgga gaatggatgc actcatattt
     1321 cttttctttc catcggggac ggcctgaaca tcatgaaaca gcgctttgac gggggagcga
     1381 aagccgtccg cgagttccgg ccgggtaccc cataccatta tgtcaactgc agtcccgatc
     1441 ccaataccgc ccgcatcctt attcagaaac tgttgacctc agcaaaccag cctgacggca
     1501 tcattggcgc ggtggaaaaa ctggccattg ccacctacct ggaatgcaaa agcctggaaa
     1561 taaggatacc tgaagactta cgagtccttg ccttttcaaa cctgcctacg gcttccctcc
     1621 ttaaacccgg attgtcaacg atccggcagc cggcctatga gatcggaaaa aaggccgcaa
     1681 gccttttgtt tgaaagcctg aataaaaaca agccgccctt cacccctaag tccattgtag
     1741 ttccttccgt tctggagccc agggcatcca cattatggaa agaatggcag gaccatcaat
     1801 aacccgggtt ctgaacgagg tagcccggaa tgtttgaggc gccgtcaatg gcagtctgcg
     1861 ggatcgggaa aacccttttg tttttatccg tattgtcctt ttccgtccag gtcccttcgt
     1921 attttccaaa gcggatcata tcggtccgtc tcagcatttc ccagtaaaac tcgaatcccc
     1981 gctcgcggta taagaggtcc agcgacatgg aggtcagggc aggaggcgga gtggtcagat
     2041 tgcgggatgc ccggaccgta ttgacgtcgg ccagggcgga cgcagcgtcc ccgcttttcc
     2101 gaagtttggc ctcagcccgc atgagataaa tatccgccag ccttacgatg acgatatcgg
     2161 cttccccgaa attcctgccg ctggctgatt ttttactgaa ttcgtatttc tccacccggt
     2221 agccggtgtt gtaattgctg ccctccacgg taaaatcgat tctctcggtg aaatccacag
     2281 ccagggtggg tttgttccgg gtatcgtgaa agagcgggcc gactttaaat ttcccatcgg
     2341 cgcatttcag aaaaacccca tttctacgga tcaggccata ctgctgtccc cggaggatac
     2401 cccggttgat ccggaaactg gctccgtcga cacaggaatc ggcaggattg ctgtagatcg
     2461 acaggttttg ctgataaaag cgggggtcca ccgaaggatc ggccggagca taagcctggg
     2521 cccaggtctg gtaaaaatca gaggtaatgc ccggcccatc cgtaccattt gccgccggga
     2581 aagccggcag gggaaactgg tcgccggaaa gggaaaaata agccagacgg ttgtgcccgt
     2641 tgaggtcggc cctctggtcc accgcaaaaa tcaattcctt attggtgtga ttttcatcat
     2701 tgaagatgga aaaataatcc cgcgacaggg tgtagaggtt cgaaccgatg attttatcgc
     2761 aatattcgat caccttgtcc atatcctcct gcggaaaagc aaaggtcgcc gaataggggg
     2821 cccggtaaac ggccccgttc aggtagagcc ttgccagcaa tcccca
//
LOCUS       DAOQXT010000218         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_37774_length_2854_cov_5.999643, whole genome shotgun sequence.
ACCESSION   DAOQXT010000218 DAOQXT010000000
VERSION     DAOQXT010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37774_length_2854_cov_5.999643"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PKM27_19470"
     CDS             <1..1225
                     /locus_tag="PKM27_19470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR09509.1"
                     /translation="LTGRKRQTLVSILGVMFGITVFIFQAGMITGLQNYFVEKTIQST
                     PHIQISNETTASEKPVAAKAYPENRDIWFSIRNQKDRLEEDKIKDGLQIASLLENHPE
                     VYGVSPSLTSQAIFKLGVLDVSAIVHGVDILRENRLFNLEKDIVQGSVIRLETVNNGL
                     ILGSGLAEKLGANLNDNLNIVSPNGVSLPMKVVGILSTGLRDLDDQRAYSSLRNAQKL
                     MKAQSSHITDISIKLRNIDKAPELADEFENRFGYRAVDWKEKNAAVFSVFKIQNIVTY
                     LVILSILLVSGFGIFNILTMMIYEKMTDIAILKAIGYTDKDIRRIFLSEAMLIGLAGG
                     VLGLITGLVISIIAASIPFRIEGFVSSERLNINFNPLFYTIAFIFGLLTTAIAGLLPA
                     RKAAKVDPLHIIRNQ"
     gene            1225..1899
                     /locus_tag="PKM27_19475"
     CDS             1225..1899
                     /locus_tag="PKM27_19475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR09510.1"
                     /translation="MPEKTILEARHLDKYFYDPVKFHVLKDINLRVEEGEFVSIVGSS
                     GSGKSTLLYTLSSLDTDYEGEVIIDQTALRSLSNDDLARLRNRSIGFIFQSHYLLADF
                     TAIENILLPGLKLGKRSKPDLLRAALEKMDLLGIRDQADKAVNTLSGGQQQRVAIARA
                     LINDPKIIFCDEPTGNLDSGNARIVFDLLKDLAHNYKQTILMVTHDESYQTDRQIRLH
                     DGRVVD"
     gene            2485..2844
                     /locus_tag="PKM27_19480"
     CDS             2485..2844
                     /locus_tag="PKM27_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09511.1"
                     /translation="MASILNEYINKRLGLLDLQNELQRLIKLYNKHTGRYMFIYAADI
                     NKARSRGIDVSMVQDDFYNIQDILRESKEKKIDFYIETPGGSGEAAEEIAKFLRKQFD
                     EVNLYPPGQVHILGLAV"
BASE COUNT          828 a          672 c          660 g          694 t
ORIGIN      
        1 ccttaccggg cggaagaggc aaaccctggt atccatactg ggagtgatgt ttggcatcac
       61 ggtctttatt tttcaggcgg gaatgattac cggcctccag aattattttg tagaaaagac
      121 catccagtcg accccgcaca ttcaaatctc caacgaaacc acggcttcag aaaaaccggt
      181 cgctgccaaa gcctatccgg aaaacaggga catctggttt tccatcagaa atcaaaagga
      241 ccggctggag gaggataaga ttaaagatgg cttgcaaatc gcgagcttat tggaaaatca
      301 cccggaagtg tatggcgtat ccccctctct caccagccag gccatcttta aattgggggt
      361 gctggatgtt tcggccatcg tacacggcgt ggacatccta agggagaacc gcctcttcaa
      421 cctggaaaaa gatatcgttc agggttcggt gatccgcctg gaaaccgtca acaatgggct
      481 catccttggt tccggacttg cggagaaact gggggcaaac ctgaacgaca acctgaatat
      541 cgtatccccc aacggggtca gcctcccgat gaaggtggtc ggaatccttt caaccggcct
      601 gagggacctg gatgatcagc gggcctattc cagtctgcgc aatgcccaaa aactgatgaa
      661 ggcccagagc agtcacatta ccgacatcag catcaaactg aggaatatcg ataaggcccc
      721 ggaactggcc gacgaattcg aaaaccggtt tggctaccgg gcggtagact ggaaagagaa
      781 gaatgccgcg gtgttcagcg tttttaaaat ccagaatatt gtgacctacc tggtgatcct
      841 ttccatcctg ctggtctccg gtttcgggat cttcaatatc ctcaccatga tgatctacga
      901 aaaaatgacc gacatcgcca ttttaaaggc cataggctat accgacaaag acatccggcg
      961 gatttttctg tccgaagcga tgctcatcgg gcttgcagga ggggttttgg gtttgatcac
     1021 gggcctggtc atttccatta ttgccgcgag catccccttc aggattgaag ggttcgtaag
     1081 ttcggagcgg ctcaatatca attttaatcc gctgttttac accatcgcct ttattttcgg
     1141 cctgctgact acggccattg caggattatt gccggcccga aaagcagcca aagtcgatcc
     1201 actccacatc atccggaatc aataatgcct gaaaagacca tcctggaagc aagacacctg
     1261 gacaaatatt tttacgatcc ggttaaattc catgtgctga aagacatcaa tctcagggtg
     1321 gaggaagggg aatttgtttc cattgtgggc agttccggga gcggaaaatc aacgcttttg
     1381 tacacgctct cttccctgga caccgattac gaaggcgagg tcattattga tcagacggcc
     1441 cttcgctccc tcagcaacga tgacctggcc cgattgcgca accggtccat cggttttatc
     1501 tttcaatcac attatctgct ggcggacttt acggctattg aaaacatcct tttgccgggc
     1561 ctgaaactgg gcaagcggtc aaaacccgac ctcctccggg ctgccctgga aaagatggac
     1621 ttgctgggca tccgggacca ggcagacaaa gcggtcaata ccctttccgg agggcaacaa
     1681 caacgcgtgg ccatcgcccg ggccctgatc aatgatccga aaatcatatt ttgcgacgag
     1741 cccaccggga atctcgattc aggcaatgca cggatcgtat ttgacctgtt gaaggacctc
     1801 gcccacaact acaagcagac gatcctgatg gtcacccacg atgaatccta tcaaaccgat
     1861 cgccagatcc ggttgcacga tggacgggtg gtagattgag ttaaacactc cgagggggca
     1921 ggccccaagg ggggcagggc aagctcaccg gtataaagaa cagttcatca gtttaacagt
     1981 tcaccagttg gacagctcga cggtctgtgg cagagcttgc gaaaagccag ttcaaacggc
     2041 tacaaacggc ttcacccgca tacaaccgga ttcaaatgac tacaaccgta ttaagacttt
     2101 aatatcctaa acggacataa gccttctcac ctaaaattat ataaacactt gatttattta
     2161 tacttattat tattttgaag gcataatttt atatcgggac ttcgaagcaa ccaaagagga
     2221 tttatttact tctacaatac gcatgaacaa cataaattat tcatatagaa tcctttatac
     2281 ccaatttgaa tggactttac tggctaagtc gtttgtggaa ttcaaatccc ggattcaacg
     2341 ccggcatcgc atttagtgat gatatataat atttaacgca atattgcgta aaagcagatg
     2401 ttggcggtca tgctatgacg acagtgtaat tttgaaacat ttgacaaata aatattttaa
     2461 cttttacaaa aataatttca gacaatggca agcatcttaa acgagtacat taacaagcga
     2521 ctgggattgc ttgacctaca aaacgagttg caaagactga taaaactata taataaacat
     2581 acaggacgtt acatgtttat ttatgcagcc gacattaaca aagcacgaag cagaggaatt
     2641 gatgtaagta tggttcaaga cgatttttat aacatacaag atattttacg agagtctaaa
     2701 gaaaagaaaa ttgatttcta tattgagaca cctggaggca gtggagaggc ggctgaagaa
     2761 attgcgaaat ttctcagaaa acaatttgat gaagtaaatt tgtatccacc cggccaagta
     2821 catatactcg gattagcggt atgaataaag aagg
//
LOCUS       DAOQXT010000219         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_39497_length_2772_cov_4.677586, whole genome shotgun sequence.
ACCESSION   DAOQXT010000219 DAOQXT010000000
VERSION     DAOQXT010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39497_length_2772_cov_4.677586"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKM27_19485"
     CDS             <1..229
                     /locus_tag="PKM27_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09512.1"
                     /translation="AFKYFRQESDGSFTPQRFRDSNRGKWMTMAKADLDRDGDQDIVL
                     GSFILNTDGVTGTREELQNYALVYLENRGRR"
     gene            758..831
                     /locus_tag="PKM27_19490"
     tRNA            758..831
                     /locus_tag="PKM27_19490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:792..794,aa:Pro,seq:ggg)
     gene            853..926
                     /locus_tag="PKM27_19495"
     tRNA            853..926
                     /locus_tag="PKM27_19495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:887..889,aa:Pro,seq:cgg)
     gene            985..1059
                     /locus_tag="PKM27_19500"
     tRNA            985..1059
                     /locus_tag="PKM27_19500"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1020..1022,aa:Val,seq:gac)
     gene            1079..1723
                     /locus_tag="PKM27_19505"
     CDS             1079..1723
                     /locus_tag="PKM27_19505"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNR09513.1"
                     /translation="MYKAIKQYCDLRNVELLVVSKMRSDEEIMQVYREGQRLFGENRV
                     QDLLLKKDRLPADIQWHLIGTLQRNKVKYIAPFISMIQSVDDLPLWQEIHRQAVKTNR
                     VIPCLLQVKIAREESKEGFDPHELHRYLADGLWRQWNGVSVRGLMGMASLTPDQEQWK
                     REFRTLRAEFESIRDNYFQGPEFNILSMGMSADYKIAIEEGANLVRVGSAVFAS"
     gene            complement(1724..>2772)
                     /gene="tyrS"
                     /locus_tag="PKM27_19510"
     CDS             complement(1724..>2772)
                     /gene="tyrS"
                     /locus_tag="PKM27_19510"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNR09514.1"
                     /translation="FNDSVDNIYLTEEELDRNLAFQQSQFLKYLDFEQGPNAAIFVNN
                     YDFYKELNVLDFLRKVGKHLTVNYMLSKESVQKRLETGISFTEFSYQLLQGYDYYCLL
                     KKFGCTLQMGGSDQWGNITAGTEYIRRTTDGVEVHALTSPLLTKADGKKFGKSESGNI
                     WLDPKMTSPYKFYQFWINADDQDLPRFLRTFSLKDMETILHLESLISSDPRTVKKELA
                     EELTRRIHSDGDFEAAMQVSDLLFNPKVDADFLNRLTDNQYSDISEEIPSYFLSGKNL
                     NGLPVIDLLSGATTICQSKSMARQAILNQAISINKVKVNGLDEVLFSDQPIRGKWLLL
                     ENGRKNKYLVVIRD"
BASE COUNT          727 a          601 c          720 g          724 t
ORIGIN      
        1 cgcattcaaa tatttcaggc aggagtccga tggaagcttt accccgcagc gctttcgcga
       61 cagcaaccgg ggcaaatgga tgacgatggc caaagccgac ctggaccggg acggggacca
      121 ggatattgtc ctggggtcat ttattttgaa tactgatggg gtgaccggga cccgggaaga
      181 attgcaaaat tatgctttgg tttacctgga gaatagaggt aggaggtagg aggtaagagg
      241 ttagaggtaa gaggttagag gtaagaggtt agaggtagga ggtgagagga cttgggaaaa
      301 taaaaaggca acatattttt caatatgttg cccaaaaagt tgggtttaca tttacatctc
      361 aggttgaatt tcgatgaaaa taagaaacgg ttttacccgt ttcaaataaa cccatccggt
      421 actgacaacc agaacttgtc aggttgtatc caggcagtgc gcaaatgccc aaaggtatct
      481 gtgcataacc aagttcgata caagggtttt ctgtctggga tgctagatat agaccggaga
      541 cctaccttta cttataaagg aagaccttga atcgacaatt aaagttatac taaattcttt
      601 cgaaaagaca agtcataagt gttaaaattt caatatttaa tatatgggaa aaatatatat
      661 gaagccttcc ggccatacgg ccgggggtta atctgtagtt tttcaataag aaggcgggca
      721 aactgaaaaa tatattttat ttttgcgggt cattagacgg ggtgtagcgc agtccggtag
      781 cgtatcagca tggggtgctg gtggtcgctg gttcgaatcc agtcaccccg actaaatttt
      841 taatgatctg tgcgggatgt agcgcagccc ggtagcgtgc ttcgttcggg acgaagaggt
      901 cgctggttcg aatccagtca tcccgactga ttatatttat gtattaccgg ccttcatcaa
      961 cggatgcggc cgggtatgtt tgaagggtgt ttagctcagt tggttcagag cgctactttg
     1021 acagagtaga ggtcactggt tcgagcccag taatacccac ttttcctaaa ttaaaacatt
     1081 gtacaaagcg ataaaacaat attgcgattt gcgcaatgtt gaattactgg tggtatccaa
     1141 aatgaggtcc gatgaggaaa tcatgcaggt gtaccgcgag ggccagaggc ttttcgggga
     1201 aaaccgggtc caggacctgc ttttaaaaaa agaccggctg cctgccgata tccaatggca
     1261 cctgatcggg accctgcagc ggaataaagt aaaatacatc gcccccttca tcagcatgat
     1321 ccaatccgtg gatgaccttc cgttatggca ggagatccat cgtcaggccg ttaaaacaaa
     1381 ccgggtcatt ccctgtcttc tccaggtcaa aatagcccgg gaagaatcca aggaaggatt
     1441 cgacccgcat gaactgcacc ggtacctggc agatggactc tggcgtcaat ggaacggggt
     1501 atccgtccgg ggactgatgg gcatggccag cctgaccccg gatcaggaac aatggaagcg
     1561 ggaattcaga accttgcgtg cggagtttga atccatcagg gataactatt ttcaggggcc
     1621 ggaattcaat atcctgtcca tgggtatgtc tgccgactat aaaatagcca ttgaggaagg
     1681 ggccaacctg gtaagggtag gcagtgcggt ttttgcttcc tgatcaatcc cggatcacca
     1741 cgagatattt gtttttccgg ccattttcaa gcaacaacca tttcccccgg atgggctgat
     1801 cgctaaacaa tacttcgtcc agtccgttta ctttcacctt atttattgaa atggcctggt
     1861 taaggatagc ctggcgggcc atggatttgg attggcaaat ggtggtggcc ccggacagca
     1921 ggtcaatcac cggaagacca ttgaggtttt tccctgaaag gaaatacgaa gggatttctt
     1981 ccgagatgtc gctatactgg ttgtccgtga gccggttcaa aaagtctgcg tccactttag
     2041 ggttaaacaa caggtcgctg acttgcatcg ccgcttcgaa atccccatcc gaatggatcc
     2101 tgcgggtgag ttcttccgcc aattcttttt taacggtcct cggatccgat gaaatcaggc
     2161 tctcgaggtg aagaatggtt tccatgtcct tcagggaaaa ggtcctgaga aagcggggca
     2221 ggtcctggtc gtcggcattg atccagaatt ggtagaattt ataggggctg gtcatcttgg
     2281 gatcgagcca gatattaccc gattccgatt ttccgaattt tttaccgtcc gccttcgtca
     2341 gcagcgggga agtgagggca tgcacttcca cgccatcggt cgtccgtcgg atatattccg
     2401 tgccggcagt gatatttccc cattgatcgg atcctcccat ctggagggtg catccgaact
     2461 ttttcaataa acaataataa tcatagccct gaagcagctg gtagctgaat tccgtaaagg
     2521 agatgcccgt ttccagcctc ttttgtacgg attccttgga cagcatataa tttaccgtca
     2581 ggtgtttgcc caccttcctt aaaaagtcca ggacattcag ttctttataa aagtcataat
     2641 tgttcacaaa tatggctgca ttgggcccct gttcaaaatc aagatatttc aggaactggg
     2701 attgctggaa agccaggttc cggtccagtt cttcttcggt caggtatatg ttatcaacgg
     2761 agtcgttgaa ca
//
LOCUS       DAOQXT010000220         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_39856_length_2756_cov_6.340244, whole genome shotgun sequence.
ACCESSION   DAOQXT010000220 DAOQXT010000000
VERSION     DAOQXT010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39856_length_2756_cov_6.340244"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..422)
                     /locus_tag="PKM27_19515"
     CDS             complement(93..422)
                     /locus_tag="PKM27_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR09515.1"
                     /translation="MGLTKTDFFTDQQNRLATLLKALAHPARIAIIQHLIKDQHCICS
                     NLVDELGLAQATISQHLKELKSIGIIKGCIEGNSMRYCIDENVWKQIQHEWDAFFGSY
                     QSLKAGP"
     gene            complement(476..955)
                     /locus_tag="PKM27_19520"
     CDS             complement(476..955)
                     /locus_tag="PKM27_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR09516.1"
                     /translation="MSATEAYLQTWKEARTRFSNLLEGLQEEDLPKKLINTKNSAGFL
                     IRHISDVELLFAKNVFGASALAVHAKTLIAQRDTGEWTNLKELMDYQIFAYESLKSIL
                     EKVSTEDWLQTITTREFGTKTKAEALGRIISHTAYHAGQLALTIRYGSDATGRFPTV"
     gene            complement(961..1557)
                     /locus_tag="PKM27_19525"
     CDS             complement(961..1557)
                     /locus_tag="PKM27_19525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR09517.1"
                     /translation="MSEFWNERYREKDYVYGEEPNVFFKEQIQKLTPGTVILPCEGEG
                     RNAVYAASLGWQVHAFDSSAEGRRKALLLAEKKGQAIEYAAEDALTAHYPDESAEVVA
                     FIYAHFVPQVRKHILPESVRWLKSGGRIILEGFNPLQLKNHSGGPKEPAMLYTEDMLR
                     EDFAGLKIEWLQSLQITLKEGKYHQGLADVVQFVGIKG"
     gene            complement(1580..2581)
                     /locus_tag="PKM27_19530"
     CDS             complement(1580..2581)
                     /locus_tag="PKM27_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09518.1"
                     /translation="MLKKMLLGILAVLVVAFIGFYTYVSLNWNKKYDWPGPALKSSTD
                     SAVIARGNYLVNGPAHCRSCHISSFEDLVASDEGKEVDLKGGIGFPMGPLGTIYTRNL
                     TPDKKTGIGRYSDEQLFRMMRHGIRPDGQASLPLLMPFWNMADDDLVAIVSYLRSLKP
                     VEHSVPENQYTFMGKAVRSLTSTFKPIEKNQAPAVAPPMAATVERGEYLARYVTNCVG
                     CHTSRDLMTYEATGPEFAGGMEFEPWPELHKFMKADPEEWLRTPNITPDPDGALAKFK
                     TPEEFIQRFRLGRVIPHSPMDWGPFSRMSDEDITALWMYLNSLAPVKNEVGAIQFKK"
BASE COUNT          656 a          707 c          638 g          755 t
ORIGIN      
        1 ccagcttttt cctttccatg tttgttgaag tttgttaacc tatatcatat attggtttat
       61 tgcaatattg cgatggttta tgggcaaaaa aatcagggac ctgcttttaa agattgatat
      121 gaaccaaaaa atgcgtccca ttcatgctgt atctgtttcc acacgttttc atcaatacaa
      181 taacgcatac tattgccctc aatacaaccc ttgatgatcc cgatgctttt caattccttt
      241 aagtgctggg aaatggtcgc ctgcgccagg cctaattcat cgaccaggtt gctgcaaata
      301 caatgctggt ctttgatcag gtgttggata atggcaatcc gggccgggtg cgcgagggct
      361 ttcagcaagg tggccagcct gttttgttga tcagtgaaaa aatccgtctt ggtcaagccc
      421 ataacatcgc aatattacga tattgcaggc tttccaccaa atccatctcg atcaattaaa
      481 ccgtcggaaa tctcccggta gcatccgaac catacctaat tgtcagagcc agttgcccgg
      541 catggtaagc ggtatgtgaa atgatcctcc ccagcgcttc ggctttggtt ttggtcccaa
      601 attcccgggt ggtaatggtc tgcagccagt cttcggttga gactttttcg agaatcgatt
      661 tcaggctttc ataagcgaaa atctggtaat ccatcaattc tttcaagttg gtccattcac
      721 cggtatcccg ctgggcgatc agggttttag catgaaccgc aagagctgag gctccgaata
      781 cattttttgc aaacagcagt tccacatccg aaatatggcg aatgaggaat cctgcgctgt
      841 tttttgtatt gattaacttt ttgggtaaat cctcctcctg caggccttcc agcaggtttg
      901 aaaaccgggt cctggcctct ttccaggttt gcaggtaagc ttccgttgca gacatttgaa
      961 tcaacctttt attccgacaa attgaaccac atcggccaat ccctggtgat atttcccttc
     1021 tttcagggta atttgaagag attgaagcca ttcgatcttc aatcccgcaa aatcctcacg
     1081 cagcatatcc tcagtataca acatggccgg ttcctttggt ccgcctgaat ggttttttaa
     1141 ttgcagggga ttaaagcctt ccagaatgat ccggccgccg cttttcaacc atcggacaga
     1201 ctccgggagt atgtgtttcc ggacctgggg aacaaaatgc gcataaataa aagcgacgac
     1261 ctccgcgctt tcatccgggt aatgcgccgt caaggcgtct tcggccgcat attcgatggc
     1321 ctggcccttt ttttcagcca acaggagggc ctttctcctt ccctccgcac tggaatcaaa
     1381 agcatgcacc tgccagccca gggaggccgc atatacggca ttcctgccct cgccttcaca
     1441 gggcaaaatg acggtgcccg gtgtcaactt ttggatttgt tccttaaaaa atacgttggg
     1501 ttcttcaccg taaacgtaat ccttctcccg gtatcgttcg ttccaaaatt cgctcaaggg
     1561 ctacactgaa ttgacccgtt tattttttaa actgaatggc ccctacctcg tttttcaccg
     1621 gagccaggct attgaggtac atccataaag cggtgatgtc ttcatccgac atccgcgaaa
     1681 aaggccccca atccataggg gaatgaggaa tgacccttcc cagcctgaaa cgctgaataa
     1741 attcttcggg cgttttaaat ttagccagag ctccatccgg gtccggggtg atattcgggg
     1801 tccggagcca ttcctcggga tccgctttca taaacttatg caattcaggc cagggctcga
     1861 actccatgcc ccctgcaaac tcagggcctg tggcctcata agtcatcagg tcccggctgg
     1921 tgtggcagcc tacacaattg gtcacataac gggccaggta ctctccccgt tccacggtag
     1981 ccgccatcgg cggtgcaacg gcaggagcct ggttcttttc aatcggcttg aaggtggaag
     2041 tcaggctgcg caccgcttta cccataaacg tatattggtt ttcagggact gagtgttcca
     2101 ccggtttaag cgaacgcagg taagagacga tggctaccag atcatcgtcc gccatattcc
     2161 agaaaggcat cagcaagggc aggcttgcct ggccgtccgg ccggatgccg tgacgcatca
     2221 tccggaaaag ctgttcgtcc gaataccggc cgatcccggt ctttttatcg ggagtaagat
     2281 ttcgggtgta aatggtacct aaggggccca ttggaaaacc tatccctcct ttaagatcta
     2341 cctcttttcc ttcatccgag gctactaaat cttcaaagct tgaaatatgg caggacctgc
     2401 agtgggcggg tccgtttacg aggtaatttc cgcgggcgat caccgccgaa tcggtactgg
     2461 acttcaatgc aggtccgggc caatcgtatt ttttattcca gttgagggag acataggtgt
     2521 aaaatccaat gaacgccacg acgaggacgg ccaggatgcc aaggagcatt tttttgagca
     2581 tgattaagtt tggagttttt cgatcagcag cttcaccggc tggaattcag caatccggcc
     2641 tttgtctgtt gaaggtaagg aaaccggcct tttccgccaa atcccattaa aaaccttgtt
     2701 cggacgaatt tctacgatcc tccaaatctt ttatgatatt tatttctatg cataat
//
LOCUS       DAOQXT010000221         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_40268_length_2737_cov_4.824385, whole genome shotgun sequence.
ACCESSION   DAOQXT010000221 DAOQXT010000000
VERSION     DAOQXT010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40268_length_2737_cov_4.824385"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..602)
                     /gene="rpsO"
                     /locus_tag="PKM27_19535"
     CDS             complement(339..602)
                     /gene="rpsO"
                     /locus_tag="PKM27_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNR09519.1"
                     /translation="MSKEKVQEIMTQYGGRPENTGSVEAQIALFTFRVNQLQEHLNQN
                     RKDHSCRRSLLTIVGKRKQLLNYLAKQNLSKYRELIEQLGIRK"
     gene            794..2071
                     /locus_tag="PKM27_19540"
     CDS             794..2071
                     /locus_tag="PKM27_19540"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNR09520.1"
                     /translation="MMKFDRKVLGNGLTVLLHQDESTPLASVNVHYFVGSSDDPPDQT
                     GMAHLFEHLMFSGTRNVPNYDQAVQNAGGENNAFTSSDSTNYHITLPSENLEMALSLE
                     ADRMQGLILNRGIFNREKQVVLEEYKETCLNKPYGDSFHLVSGLAYHRHPYRWPTIGL
                     NEKMISHLKLTVARKFYNTYYQPANAVLSIASNRPPEQLFPLVEKHFGPVKSKGRPVK
                     KHTKEPDQKKSRVLTAAKKAPAEALYLAFHMEGRGHPDFYVADLISDVLSNGQSARLF
                     RRLVRGKKLVSMIDAYITATLDPGLFMIEAKAIPGQPIKKVLKAIWEELRLLKTERVP
                     AGELNKMKNTIEASLEFSEINGLNKAITLGYFEMLGDANRANLEYGIYKKVSPEDILR
                     VARKLFRHSKASTLFYKKANDGQGYFDEEEEED"
     gene            complement(2077..>2737)
                     /locus_tag="PKM27_19545"
     CDS             complement(2077..>2737)
                     /locus_tag="PKM27_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HNR09521.1"
                     /translation="GQDYKILPVAKDYKKIGEADRGPNTGGMGAISPVSFVDPAVMEK
                     VESRIIRPTIRGIHQRGLKYQGFVFFGLIEVDGEPLVIEYNCRMGDPETEVVMPRLEN
                     DLLELLVATHRHQLGSQVIRESQLYAATVMLVSGGYPGDYEKGKPIGLPDFTHDSILF
                     HAGTTTDTRSGLVTAGGRVMAVTSMAVDKNTALAMSYSIIPHIHFEGKNFRRDIGFDV
                     E"
BASE COUNT          687 a          694 c          694 g          662 t
ORIGIN      
        1 cataatctat tgagagtgga aaatagtcca gctcaggctt gggttccttg ctggatacca
       61 ccgtggccag gaggatgatg ttgccgcaat acaatacgac ggagccatgc gcttgcgtgg
      121 ccattttgcc agtttccaac cggatttctc taccgtccgg gagattaagt gaaactgaaa
      181 caggagtttt ttttcccata aattttttag aataattaag gttaaactga aaaggctcta
      241 acgggtagag cattcgaaca acagaacatg gaaataatcg aaggaaggtg ggcaggcggg
      301 caagcaagcc ggttttttcc ggattggttg cacctgggtt atttacggat acctaactgc
      361 tcgatcaatt cacggtattt actcagattt tgcttggcca ggtagtttag caattgttta
      421 cgttttccta cgatggtcag gagggatctg cggcaggaat ggtctttcct gttctggttg
      481 agatgctctt gtaactgatt gactctaaat gtgaaaagag caatctgggc ttcaacagaa
      541 cctgtgtttt caggcctgcc cccgtactga gtcatgattt cttgtacttt ttctttggac
      601 atgtaagccg gcatacgatt ttttggcttt tacttttaat taatttgtta tttcgggcgc
      661 aaagatatag tttttaatga actgattctt tgaattttac atttttttta agccgggcgg
      721 ccgggttttt ggggatggat ggcgggctcg gggaggattt ggcaaaaccg ccctattttg
      781 cagccttgat tttatgatga aattcgaccg taaggtactt ggcaatggcc tgaccgtcct
      841 cttgcaccag gacgagtcaa cgcctctggc ctccgtcaat gtccattatt ttgtaggttc
      901 gagcgacgat cctcccgacc agaccgggat ggcccacctt tttgagcacc tcatgttcag
      961 cggtacccgg aacgtgccca attatgacca ggcggttcaa aacgcggggg gcgaaaacaa
     1021 tgccttcacc tcctctgaca gcacaaacta tcacatcacc ctgccatccg aaaaccttga
     1081 aatggccctt tccctggagg ccgaccggat gcaggggctg atcctcaacc gggggatttt
     1141 caaccgggag aaacaggtgg tcctggagga gtataaggag acctgcctga acaaacccta
     1201 tggggacagt tttcacctgg tttccggcct cgcttaccac aggcatcctt accgctggcc
     1261 gacgattgga ttgaatgaaa aaatgatcag ccacctaaaa ctcaccgtgg cccgcaaatt
     1321 ttacaatacc tactaccagc cggcaaacgc ggtcctgtcc atagccagta accggccccc
     1381 ggaacaactt ttccccctgg tcgaaaaaca tttcgggcca gtgaagtcca agggcaggcc
     1441 ggttaaaaaa cataccaaag aaccggatca aaagaaatca agggtactta ccgcagccaa
     1501 aaaggcgccc gcggaagccc tttacctggc atttcacatg gaaggaaggg gacatcctga
     1561 cttttacgtt gcggacctca tcagtgatgt actatccaac ggccagtccg caagattgtt
     1621 cagaagattg gtccgcggta aaaaactggt cagcatgatc gatgcctaca ttaccgccac
     1681 cctggacccc ggactgttta tgatcgaagc caaggcgata ccgggccaac ccataaaaaa
     1741 ggtgctgaag gccatctggg aggaactccg tctcctgaaa accgaaaggg taccggccgg
     1801 cgaactcaat aagatgaaaa atacgatcga ggcttccctg gaattcagcg aaatcaatgg
     1861 cctgaacaaa gccatcaccc tgggttattt tgaaatgctg ggcgatgcaa accgggccaa
     1921 cctggaatac ggcatctata agaaagtgag cccggaagat attctgcggg tagcccgtaa
     1981 acttttccgc cattccaagg ccagtaccct gttttataaa aaggccaatg acggacaggg
     2041 ttattttgat gaagaggaag aagaggattg acggacctat tccacgtcaa aaccaatgtc
     2101 cctgcgaaaa tttttcccct caaaatggat gtgagggatg atggaatagg acatggccag
     2161 ggcggtattc ttgtccacag ccatagaggt aacagccatc accctgcccc cggccgtgac
     2221 caggccggac cgggtatcgg tcgtcgtacc ggcatgaaac aggattgaat cgtgcgtaaa
     2281 gtcaggcagc ccgatcggct tgcccttctc atagtctccg ggataacctc cactcaccag
     2341 catgacggtg gccgcgtaca attgagattc ccggatcact tgtgacccaa gctgatgccg
     2401 gtgggtcgcc acgagcagtt ccaggaggtc gttttcaagt cggggcatga ccacttcggt
     2461 ttccggatcc cccatgcggc agttgtattc gatcacgagg ggttcgccgt ccacttcgat
     2521 caggccgaaa aatacaaagc cctggtattt aagacccctt tgatggattc cccggatggt
     2581 tggccgtatg atccggcttt ccaccttttc catgacggca gggtccacaa aggaaaccgg
     2641 ggatatggcc cccatccccc ccgtattggg tccgcggtcg gcttcgccga tctttttata
     2701 atccttggca accggcagaa tcttgtagtc ctggccg
//
LOCUS       DAOQXT010000222         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_43206_length_2614_cov_5.338413, whole genome shotgun sequence.
ACCESSION   DAOQXT010000222 DAOQXT010000000
VERSION     DAOQXT010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43206_length_2614_cov_5.338413"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PKM27_19550"
     CDS             complement(<1..417)
                     /locus_tag="PKM27_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HNR09522.1"
                     /translation="MTVDRLIDKALRREFLSIGEGAFLYRSASLGILMAAAHRLRREL
                     VPGDAVTWIIDRNSNTTNVCVANCKFCNFYRVPGHEEAYITSLDEYRVKIEELFRYGG
                     EQLLLQGGHHPDLGLDYYCNLFRELKRMYPRLKLHAL"
     gene            580..1050
                     /locus_tag="PKM27_19555"
     CDS             580..1050
                     /locus_tag="PKM27_19555"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HNR09523.1"
                     /translation="MIEETVTAWLNEKFTEESFRDCFIVEVQFKQRRLAVYLDSDKGL
                     NLDRCAAISRWLGHKIEEIDLIPDAYTLEVSSTGLDRPLQQYRQYLKNIGREVTVHLE
                     SGKTLEGKLTEVRDGLIVLTSEVVEKEKNKKIKKNVETLIHLSDVKKTFIQIKF"
     gene            1054..2307
                     /gene="nusA"
                     /locus_tag="PKM27_19560"
     CDS             1054..2307
                     /gene="nusA"
                     /locus_tag="PKM27_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNR09524.1"
                     /translation="MINLVETFSEFKAGKNIDRPTLMRVMEDVFRTLIKKKYGTDENF
                     DVIVNTQKGDLELWHVRQIVPDGEVSDPNKEISVSEAREIDPDYDIGEECYAKLELED
                     FGRRSIMAARQTLISRILELEKDEVFQKYAERKGEIILGEVNQVLPREILVIDDTTGN
                     ELVLPKSEMIKADHFRKGDMVRGVIKDVVMKNNLPSIIISRTDNAFLEKLMEHEVPEI
                     EDGLITIKNIVRIPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDIV
                     NYTNNLTLYIQRALTPAKVTKIEIDEQNKRASVFLKPDQVSLAIGKGGSNIRLASKLT
                     GFEIDVYRDTEEGAEIDDVDLDEFADEIEAWIIDTLKGIGCDTARSVLALSREELIRR
                     SDLEDETVDEVLRILSSEMEEESGS"
     gene            2351..>2614
                     /locus_tag="PKM27_19565"
     CDS             2351..>2614
                     /locus_tag="PKM27_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09525.1"
                     /translation="MAKNLVKAASEFNVGTSSIVEHLQKKGFAIDNKPNAKISDEMYD
                     ELIKEFSSSMAVKEKAEALVIGKSGSAHGPSRKEAATPPPPPPP"
BASE COUNT          742 a          645 c          670 g          557 t
ORIGIN      
        1 cagggcatgc agtttcaacc tgggatacat ccgtttgagc tcccggaata aattacaata
       61 ataatccagc ccgagatcag ggtggtggcc cccctggagc aggagttgtt cacccccata
      121 ccggaacagt tcttcgatct ttacccggta ttcatccagg ctggtaatat aggcttcttc
      181 atgcccgggc acacggtaaa aattgcaaaa tttgcagttg gccacgcaga cattggtggt
      241 attgctgttc cggtcgatga tccaggtgac ggcatcgccc ggcacgagct cccggcgcaa
      301 gcggtgggcg gcagccatca ggatgccaag ggaggcagac cggtaaagaa aggctccttc
      361 cccaatactc aggaattccc tgcgcagggc tttatcgatc aggcggtcca cggtcatcgt
      421 gcaaaggtaa tggtccggcc cgttttaagg aaaattggtg tacagaacca ttcggaagga
      481 aacctcttta ttcggaatat tttccttatc tttgcgccgt gctgatttag agttttggaa
      541 gggaacgaaa agttcccttc ttcatttaaa ccaacccgat tgattgaaga aacggtgacg
      601 gcctggctca atgaaaagtt tactgaagaa agcttcaggg attgttttat agtcgaagtt
      661 cagttcaaac aacgtcgtct ggccgtttac ctggacagcg acaagggcct gaaccttgac
      721 cgttgtgcgg ccatcagccg gtggctgggt cataaaattg aggagattga cctgatcccg
      781 gatgcctata ccctggaagt ttcttccaca ggtcttgacc gaccgcttca gcaatacagg
      841 cagtacctga aaaatatcgg acgggaagtc acggtacacc tggagtccgg gaagacgctg
      901 gaaggaaaac tgaccgaggt ccgcgatggc ctgatcgtat tgaccagtga agtggtggaa
      961 aaagaaaaga ataagaaaat taaaaaaaac gttgaaaccc tgattcattt atccgatgtc
     1021 aaaaaaacat tcattcaaat aaaattttga tccatgatca atttagtgga aaccttttcc
     1081 gaattcaaag cgggcaaaaa tattgaccgg ccaaccctga tgcgggtgat ggaggatgtc
     1141 ttccgtaccc tgatcaagaa aaaatacggt acggatgaaa actttgatgt gatcgtgaat
     1201 acccagaagg gggacctcga actctggcat gtgcgccaga tcgtcccgga tggcgaagta
     1261 tccgatccca acaaggaaat ttccgtttcc gaagccaggg aaatcgatcc ggattacgac
     1321 atcggggaag aatgttatgc caagctcgaa ttggaggatt ttggccgccg gtccatcatg
     1381 gccgcccggc agaccctcat atccaggatc cttgaattgg aaaaggatga agtattccag
     1441 aagtatgccg aacggaaagg ggagatcatc ctcggggagg tcaaccaggt cctgccccgt
     1501 gaaatcctgg tcatcgacga caccaccggc aacgaactgg ttttacccaa aagtgagatg
     1561 atcaaagccg accacttccg caaaggcgat atggtccgcg gggtcatcaa ggacgtggtg
     1621 atgaaaaaca accttccttc catcatcatt tcccgcaccg acaacgcctt cctggaaaaa
     1681 ctgatggaac atgaggtacc cgaaatcgag gatggcctga tcaccatcaa aaacatcgtg
     1741 cgcatcccgg gagaacgggc caaggtcgcc gtggagtctt acgatgaccg gatcgacccc
     1801 gtaggcgcct gtgtcgggat gaaaggatcc cgcatccacg ggatcgtccg cgaactgcgc
     1861 aatgaaaaca tcgacatcgt taattatacc aataacctga ccctgtatat ccaaagggcc
     1921 ctgacccctg ccaaagtcac caaaattgaa atcgacgaac agaataaaag ggcctctgta
     1981 tttcttaaac ccgaccaggt atccctggcc atcggcaaag ggggttccaa tatccgcctt
     2041 gccagcaaac tgaccggctt tgagatcgat gtctaccgcg ataccgaaga gggtgcggag
     2101 attgacgatg tcgacctcga tgaatttgca gacgagatcg aggcctggat cattgatacc
     2161 ctcaagggca tcggttgcga taccgccaga agcgtccttg ccctcagcag ggaggaactg
     2221 atccgcaggt cggacctgga agacgaaacc gtcgatgaag tattgaggat attatccagt
     2281 gaaatggagg aggaaagtgg aagctgagcg gcatccgggt ggatcgaatt tggtatttga
     2341 ataaaaatat atggctaaaa atttagtaaa agcagcttca gagtttaacg tcgggaccag
     2401 ttccatcgta gagcacctgc aaaaaaaggg ctttgccatt gacaacaaac ccaacgccaa
     2461 aatttctgat gaaatgtatg acgaactcat caaagagttc agttcatcca tggctgtaaa
     2521 agaaaaagca gaggcattag tcatagggaa atcaggcagc gcccacggac cttcgcgtaa
     2581 agaggccgct accccacccc cccccccccc cccc
//
LOCUS       DAOQXT010000223         2585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_43994_length_2585_cov_5.319763, whole genome shotgun sequence.
ACCESSION   DAOQXT010000223 DAOQXT010000000
VERSION     DAOQXT010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43994_length_2585_cov_5.319763"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /locus_tag="PKM27_19570"
     CDS             complement(<1..1694)
                     /locus_tag="PKM27_19570"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNR09526.1"
                     /translation="MKPVKAALAFILLILWIYLLDHPISFKSTTLPAIGNFLSPFTGF
                     WQNAVAPDVRSGTFQSGALKDKTQVLWDDRLVPHVFAEDERDAFYVSGYLVASQRLWQ
                     MDMLARSAMGRLAEVLGGERLIARDKEQRRMGMLFGAENAVRGWKQDSVHFGYIEAYC
                     SGVNDYIRQLKSRDLPLEYKLMGFTPEPWTPLKTAAIVKYMAQSLCSREYDLEAANTK
                     AFLGDSLYNLLYPDFPEGLSPVIPPGTEWGFSADTSGNATESVLDQLESLYLRPNTLP
                     PPGQGSNNWAVSGSRTQSGYPILCNDPHLRLTLPSIWFENQIKCPEFNAYGVSVPGIP
                     GILIGFNENIAWGETNVGHDVADWYRIKWADENRDAYILDGERIPVTVKIEEIRVKNA
                     KTIYDTVRYTGWGPVPVEDASSPRYGLAYHWIAHEIPQSFEMLVFLNLMKAKNYEDYY
                     RALNGYKSPAQNFVFASKDGDIALTVNGQLPIKKPGQGKYVQDGSVSANAWQGYIPYE
                     NNPRTKNPARGFVSSANQHSTDPSYPYYYNGDFEYFRGRTLNRLLTSKDRFSGEDM"
     gene            complement(1706..2467)
                     /gene="yaaA"
                     /locus_tag="PKM27_19575"
     CDS             complement(1706..2467)
                     /gene="yaaA"
                     /locus_tag="PKM27_19575"
                     /inference="COORDINATES: protein motif:HMM:NF002542.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNR09527.1"
                     /translation="MMMLISPAKKLDFKPVELREATRPRFDKETGELVKCMKKKGSRD
                     LMALMDISKALAELNVKRFDQFEAEPHEKDTKQALLAFNGDVYLGLHASDFDKKDLAF
                     AQKHLRILSGLYGLLRPLDRIQAYRLEMGSMLQVGKHKSLYSFWGDKISTLLNEDMKE
                     VKDRVLINLASEEYFHVVHPGKIEAKIIQVRFEEKRGGAFRVISFSAKKARGLMARYI
                     VKNRLSLPSGLKNFDLERYAFSPDRSSDNLYTFVR"
BASE COUNT          584 a          663 c          672 g          666 t
ORIGIN      
        1 tccatgtcct ctcccgagaa ccggtctttt gaagtaagca ggcggtttaa ggttctgccc
       61 cggaagtatt caaaatcccc attgtaataa tagggatacg aaggatcggt gctgtgctgg
      121 tttgcgctgc ttacaaagcc cctggccgga tttttggtcc tgggattgtt ttcatagggg
      181 atgtaaccct gccaggcatt ggcgcttacg gagccgtcct ggacatattt gccctgccct
      241 ggttttttta tgggcagctg tccgttaacc gtgagcgcga tatccccatc ctttgaggca
      301 aacacaaagt tttgggccgg gcttttatac ccgttcaaag cgcggtaata atcttcgtaa
      361 tttttagctt tcatcaggtt gagaaagacg agcatctcga agctctgcgg gatctcgtgg
      421 gcaatccagt gataggccag tccataccgg gggctggacg catcttccac cggtacggga
      481 ccccatcccg tgtatctcac cgtatcatag atcgttttgg cattcttaac ccggatttct
      541 tctattttga ccgtcaccgg tatccgctca ccatccagga tataggcatc gcggttttca
      601 tcagcccatt ttatccggta ccagtcggct acatcatggc ccacattggt ttctccccag
      661 gcaatgttct cgttaaaacc aatgaggatg cccgggatgc cgggcacgct tactccatag
      721 gcattgaatt cagggcattt tatctgattt tcaaaccaga tggagggtag ggtgagccgg
      781 agatgcggat cattgcagag gatcgggtag ccggattggg ttcggcttcc gctgactgcc
      841 cagttattgc tgccctggcc cggagggggc agggtattgg ggcggagata aagcgattcc
      901 aactggtcca aaacgctttc agtggcattt ccggaggtat ccgcagaaaa accccattcg
      961 gtaccgggag gaattaccgg gctaaggcct tcgggaaagt ccgggtacaa caaattataa
     1021 agggaatccc ccaggaaagc cttcgtattg gcggcttcca gatcgtattc cctgctgcac
     1081 agggattggg ccatatattt tactatggcg gctgttttaa gcggggtcca gggctcgggt
     1141 gtaaaaccca tcagcttata ctcgaggggc aggtccctgg atttcaactg acggatataa
     1201 tcattgaccc cggagcaata ggcttcaata tacccaaaat gcacactatc ctgcttccac
     1261 cccctcaccg cattttcagc gccaaagagc atgcccatcc ggcgttgttc cttgtcccgg
     1321 gctatcaggc gttctccacc gagtacttcg gccagccttc ccatggcgga tctcgccagc
     1381 atgtccatct gccacaatcg ctgcgaagcg acgaggtatc cggaaacgta aaaggcgtcc
     1441 cgctcatctt cggcaaaaac atggggtacc aaccggtcgt cccaaagcac ctgggttttg
     1501 tccttcaggg ctccggactg gaaggttcct gaacggacat ccggggccac cgcattttgc
     1561 cagaatccgg taaacgggct caggaaattc ccgatggccg ggagggtggt gcttttaaag
     1621 gaaatgggat gatcgagcag gtaaatccaa aggatcagca ggataaaggc aagggcagct
     1681 ttgacgggtt tcatccggta atgacttatc ggacaaaggt atacaagtta tcggaagacc
     1741 ggtcgggtga aaatgcgtac cgttccaggt caaagttttt caggccggag ggaaggctaa
     1801 gcctgttttt tacgatatac cgggccatca ggcccctggc ttttttagcg ctgaatgaaa
     1861 tcacccggaa cgcccctccc cgtttttctt caaaacgaac ttggatgatc ttcgcttcta
     1921 ttttccccgg atggaccacg tgaaaatatt cctcggatgc gaggttgatc agcacccggt
     1981 ctttcacctc tttcatgtcc tcattcagca gggtcgagat tttgtccccc cagaaagagt
     2041 acaggctttt atgttttccg acctgcaaca tcgaacccat ttccaggcga taggcctgga
     2101 tgcggtccag ggggcgcaac aagccataga ggccggaaag gatcctgagg tgtttctgtg
     2161 caaaggccag gtcttttttg tcaaaatcgg aagcgtgcaa tcccaggtaa acgtcgccgt
     2221 tgaaggccaa cagggcttgt ttggtatcct tttcatgggg ttcggcttca aactggtcaa
     2281 accgtttgac attcaactcg gccagggctt tgctgatgtc catgagggcc atcaggtccc
     2341 tgcttccttt ctttttcatg catttgacca gttccccggt ttctttgtca aaacgcggcc
     2401 tggtcgcctc cctgagctcc accggcttaa aatccaattt tttggccggc gagattaaca
     2461 tcatcataga ataaaattag gacgatttgt tgatttacta tggctgtgca tgctaaattg
     2521 ggtgatctgg ctgtcaatgc ctgacagagg ttgaccgttt ttaaggttga aaaacactta
     2581 aaaac
//
LOCUS       DAOQXT010000224         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_45452_length_2532_cov_6.073072, whole genome shotgun sequence.
ACCESSION   DAOQXT010000224 DAOQXT010000000
VERSION     DAOQXT010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45452_length_2532_cov_6.073072"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..>1289)
                     /locus_tag="PKM27_19580"
     CDS             complement(111..>1289)
                     /locus_tag="PKM27_19580"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNR09528.1"
                     /translation="PLSRQSMPFHFSSARNAVMNYAAQINDFSYLDGKLIFLSGSPET
                     SMAGNIPAGRLSGLVNRHIPALFKRSQLPSYATNCIEDWDTKVDAIIGECLGHDLRLI
                     SGIPPWVQMFAERILQKTGKSHIREVFPNLKLLVSGGVNFKPYESHFARLFGPDMPRV
                     DTYPASEGFIGFQYEPGDPALLLNTHAGVFYEFITIEELTKGGSHRIPLEEVKAGITY
                     AILLSTNAGLWTYNIGDTIEFVSVRPYRFVVNGRVSQYISAFGEHVIEKEVETALLAT
                     CEKHQCTVEEFTVAPQVNPDDSGKPYHEWFIDFSQTPEDMQAFARDLDAAMCLQNIYY
                     LDLIKGNILGLLRLRQVRPHGFREYMRDLGKLGGQNKIPHLRNDRLVADEMERYIVKG
                     "
     assembly_gap    1290..1389
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1468..>2532)
                     /gene="thrS"
                     /locus_tag="PKM27_19585"
     CDS             complement(1468..>2532)
                     /gene="thrS"
                     /locus_tag="PKM27_19585"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNR09529.1"
                     /translation="KRGYQPVITPHIGRKELYVTSGHYAKYGKDSFQPIHTPSEGEEF
                     LLKPMNCPHHCEIYGHKPRSYKELPIRIAEFGTVYRYEQSGELHGLTRVRGFTQDDAH
                     LFCTPDQLKQEFIGVIDLTMFVLKKLGFNQFTAQISLRDPDNKEKYIGSDENWQKAEA
                     AIIEAAAEAGLETVTVLGEAAFYGPKLDFMVKDALGRNWQLGTIQVDYNLPERFKLEY
                     IGADNQAHQPVMIHRAPFGSMERFVGVLIEHCGGNFPLWLSPEQFVVLPISDKFNDYA
                     HSVKTELLAKGFRGWVDDRSETIGKKIRDNELKKIPYLLIVGEKEADNHSISLREHGK
                     GDQGAMTLDAFAEKLDAQMT"
BASE COUNT          531 a          599 c          666 g          636 t
ORIGIN      
        1 tggtcagtga aggggaattg gtggggctgg tgactccaaa cgatattttg aggggcttgg
       61 ttaagtgaaa gttacgagtg aaaggtacga gttgaaaggt tgactctgag tcaacctttc
      121 actatatatc gctccatttc atccgccacc aggcggtcat tgcggagatg gggtatttta
      181 ttttgtcctc ccagtttgcc caggtctcgc atatattccc ggaagccgtg agggcgcacc
      241 tgcctcaagc ggagcaaacc aaggatattg cctttgatga ggtccaggta ataaatattc
      301 tgcaggcaca tggcggcatc caggtccctg gcgaaggcct gcatatcctc gggggtttgg
      361 ctaaaatcga taaaccattc gtgatagggt tttccgctat cgtccggatt tacctgggga
      421 gcgaccgtaa actcttccac ggtgcattgg tgcttttcgc aggttgccaa taaagcggtc
      481 tccacttctt tttcaatcac atgttcgccg aaagcggaaa tatactggct tacgcggcca
      541 ttgacgacaa accggtaggg cctgacggaa acaaactcaa tggtatcccc gatattgtaa
      601 gtccacagtc ccgcattggt ggagagcagg atggcataag tgatgcctgc tttcacttct
      661 tcgagtggga tccggtggga gcctcctttt gtcagctctt cgatggtaat aaattcgtaa
      721 aagactccag cgtgcgtatt cagcaggagg gcgggatcac cgggttcata ttgaaatcct
      781 ataaatcctt ccgaagccgg ataggtgtcc accctgggca tgtccggtcc aaacaagcgg
      841 gcgaaatggg attcataggg tttaaaattg actcccccgc tcacgagcag tttcaggttt
      901 ggaaagacct ctctgatgtg ggatttaccg gttttctgca aaatccgttc agcaaacatc
      961 tgtacccagg gtgggattcc gctgatgagg cggaggtcat gacccaggca ttccccgatg
     1021 atggcgtcca ccttggtgtc ccagtcctcg atgcagttgg tggcataact gggcaactgg
     1081 ctccttttga ataaagcggg gatatggcgg ttcaccaggc cagaaaggcg accggcgggg
     1141 atattgccgg ccatcgacgt ttcggggctg ccggataaaa aaatcagttt gccgtcgagg
     1201 taagaaaaat cattgatctg ggcggcgtaa ttcatcaccg cgttgcgggc gctgctgaag
     1261 tgaaaaggca tgctttgcct ggacaggggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnng ccatcgattt cacagcctgg tgtataagct gtccaaacgg ctttacaatc
     1441 gatgacttcc aagctgccat atcccgatca ggtcatttgt gcgtccagtt tttccgcgaa
     1501 agcatcgagg gtcatggcgc cctggtcccc cttgccgtgc tcgcgcaggg aaatggaatg
     1561 gttgtccgct tctttttccc ctacgatcag caggtaaggt atttttttga gttcgttgtc
     1621 ccggattttt ttgccaatgg tttcactgcg gtcgtccacc cagcccctga aaccctttgc
     1681 gagcagctcg gttttgaccg aatgggcgta gtcgttgaat ttatcgctga tcggaaggac
     1741 tacaaattgt tcggggctca gccaaagcgg gaaattcccc ccgcaatgtt cgatcagcac
     1801 acctacaaaa cgttccatgg acccgaaagg cgcccggtgg atcatgaccg gctgatgggc
     1861 ctggttgtcc gcgccgatat attccagttt gaaacgttcg ggcagattgt aatccacctg
     1921 gatggtaccc agctgccagt ttcgcccgag ggcgtcttta accataaagt ccagtttggg
     1981 cccgtaaaaa gcggcttccc ccagtacggt gacggtttcc aggccggctt cggcggcagc
     2041 ctctatgatg gcagcttcgg ctttttgcca gttttcatcg ctgccgatgt atttctcttt
     2101 attgtccggg tcccgaaggg aaatctgggc ggtgaattgg ttaaaaccca gttttttcag
     2161 gacaaacatg gtaaggtcga tcaccccaat gaattcctgt ttgagttggt ccggcgtgca
     2221 gaacaaatgc gcgtcatcct gggtaaatcc cctcacgcgg gtgagcccat ggagttcccc
     2281 gctttgttca taccggtaaa ccgttccgaa ttcagcgatg cggatgggta attccttata
     2341 cgaacggggt ttgtgcccgt agatttcaca gtggtgagga cagttcatgg gtttgagtaa
     2401 aaattcttcc ccttcgctgg gcgtgtgaat gggttggaag ctgtccttac catatttggc
     2461 ataatggcct gaggttacat acaattcttt cctcccgata tgaggagtga tcaccggctg
     2521 gtatcccctt tt
//
LOCUS       DAOQXT010000225         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_45995_length_2513_cov_6.681855, whole genome shotgun sequence.
ACCESSION   DAOQXT010000225 DAOQXT010000000
VERSION     DAOQXT010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45995_length_2513_cov_6.681855"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKM27_19590"
     CDS             complement(<1..190)
                     /locus_tag="PKM27_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09530.1"
                     /translation="MNRLLLFLLIISTASPSFSQKNTGPKPLPNGKVKDQNGKVVDLN
                     TYTKAGKITIVSFWATWCG"
     gene            252..1892
                     /locus_tag="PKM27_19595"
     CDS             252..1892
                     /locus_tag="PKM27_19595"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNR09531.1"
                     /translation="MCIRINVLLTVLLIALQSVAQDETSSGSGVFNAYLTTQANVFMR
                     DSNIGASGTPQYDHQLFGAETWLDARYSNWGFDLGLRFDLYHNSNLINPQASFNQQGL
                     GRWFIKRDISKLGFEAGYIYDQIGSGIIYRAYEQRNLGLDNALYGLKLQYRFNDYWQV
                     KAFSGKQKKQFDTYESLIKGFAIDGFVQGKDSTNWSWAPGFGFTNRTLDDGSMTNLLS
                     TLATYQSEDQFLPKYNNYAFTFFNQVQWGGLGLFLETALKSRDILNDPNGMRRNAAGE
                     IITGPKFIHKAGQVLYGSLSYSKPGWGISAEGKITRYFGIRTRPQEEANNGLIHFVPA
                     MSRQNSYRLTSFYQPATQDLGEKAFQLEGHFSPAGDWTIILHHSNINDLDGNKLYREY
                     LLESTWRKAESYTLIAGIQHQYYNQRVYEGKPLAPIVKTFIPYLDYTRQFDERRALRM
                     NVEYMHNQQDMGSWIFMLTEYSLAPRWIFTLSDMFNFKPVQGRKNNYYTAGVVYTRDA
                     ARAGINLVRQRAGIVCTGGICRYEPAFNGIKFNLESRF"
     gene            1896..>2513
                     /locus_tag="PKM27_19600"
     CDS             1896..>2513
                     /locus_tag="PKM27_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09532.1"
                     /translation="MKTIILFLTGLIFLLPACDEVPVSIPSAPDPGGQVYPQNVLIEE
                     YTGVRCQNCPAGAQLLEELKRIHGDRLIILSIHGGFFAQPTNAENKFRLDNAFGAELI
                     RLFNEPLGYPSAMISRKIFDGQSSRFLGGGFWAGYVAEELDQEPLIGLELEIVPNPDP
                     LALKIKVHVLALEVQSQVLSLSVALTENNIRDAQLTPTGVDTNYVH"
BASE COUNT          639 a          634 c          645 g          595 t
ORIGIN      
        1 ggccgcacca ggttgcccag aagctgacga tggtgatttt acccgctttg gtataagtgt
       61 tcaaatcgac cacctttccg ttttgatctt tgaccttgcc gttgggtagg ggtttgggcc
      121 ctgtattttt ctgtgaaaaa gaagggctgg ccgtggaaat gatcaggagg aagaggagca
      181 gtcgattcat ggtacgatac ttgataaaat gaggatacaa aactaagctt aaatttgttc
      241 aaaaccgccc catgtgcatc cggattaacg tccttttaac tgtattactg attgccttac
      301 aatcagttgc acaggacgaa acctcatccg gaagcggggt attcaatgct tacctgacca
      361 cccaggccaa tgtgtttatg cgggacagca atattggggc cagcggcacc ccacaatatg
      421 accaccagct tttcggggcg gaaacctggc tggacgcgcg atacagcaat tggggttttg
      481 acttgggact ccggtttgat ttataccaca acagcaatct catcaatcct caggcctcct
      541 tcaatcaaca gggccttggc cgctggttta tcaaaaggga tatttccaaa ttggggtttg
      601 aagcagggta tatctacgat cagatcggtt caggcatcat ttaccgggcc tacgaacaac
      661 gtaaccttgg cctggacaat gcactgtacg gcctgaaact ccagtaccgt tttaatgatt
      721 actggcaggt caaggctttt tcagggaaac agaaaaaaca atttgatacc tacgaatccc
      781 tgatcaaagg gtttgccatc gatggttttg tccaggggaa agattcaaca aactggtcct
      841 gggcaccggg ctttggattt accaaccgga ccctggatga cggcagcatg accaacctgc
      901 tgtccaccct ggctacctat caaagcgaag atcagtttct tccaaagtac aacaattatg
      961 catttacttt tttcaaccag gtacaatggg gcgggctggg ccttttcctg gagaccgcac
     1021 tcaaaagcag ggatatcctc aacgatccca acggcatgcg gagaaatgcg gccggggaga
     1081 tcattaccgg ccccaaattc atccacaagg cgggccaggt tttgtatggc tccctcagtt
     1141 acagcaaacc cggatggggg atctcggcgg aaggaaaaat aacccggtat ttcgggatca
     1201 ggacccgtcc ccaggaagaa gccaataacg gcctgatcca ttttgtgccc gccatgtcca
     1261 gacagaattc gtaccggctg acctcgtttt atcagccggc aacccaggac ctgggcgaaa
     1321 aagcgtttca actggaaggt catttttccc ccgccgggga ttggacgatc atcctgcatc
     1381 acagcaatat caatgacctg gatggaaata agctctaccg ggagtatctc ctggaatcga
     1441 cctggagaaa agcggaaagt tatacgctga tcgccgggat ccagcatcag tactacaacc
     1501 aacgggtata tgaaggcaag ccactggctc caatagtaaa aaccttcatc ccttacctgg
     1561 actatacccg ccagtttgat gaaaggcggg ccctgcggat gaatgtggaa tacatgcaca
     1621 atcaacagga catgggcagc tggatattta tgctgacgga atacagcctg gctccccggt
     1681 ggattttcac cttgtcggac atgtttaatt ttaaacccgt ccaggggcgg aaaaacaatt
     1741 attatacggc cggcgttgtt tatacgcggg atgccgcgcg ggccgggatc aacctggtga
     1801 ggcaaagggc cggcatagtc tgcaccggag gcatttgcag gtatgaaccc gcctttaacg
     1861 gaataaaatt caacctggaa tcgagattct gaaagatgaa aacaatcatt cttttcctta
     1921 ccggtttaat tttcctgctc ccggcttgtg atgaagtacc cgtcagtatt ccttccgctc
     1981 ctgatcccgg agggcaggtt tatccccaaa atgttttgat tgaagagtat acgggagtcc
     2041 ggtgccagaa ctgccccgcc ggcgcccagt tgctggagga actgaaacgg atccatggag
     2101 accggctgat catcctttcc attcacgggg gctttttcgc ccagccgacc aatgctgaaa
     2161 ataagttcag gctggacaat gcttttgggg cagaattgat ccggctcttc aatgaaccgc
     2221 tgggctaccc atccgccatg atcagccgta aaattttcga tgggcaatct tcccggtttt
     2281 tgggaggggg attctgggcg ggatatgtgg ctgaggaact ggaccaggaa ccgctcatcg
     2341 gcctggaact ggagattgta cccaacccgg acccgcttgc gctcaagatt aaagtccacg
     2401 tcctggcgct ggaggttcag agccaggttt tgtccttaag cgtcgcgctg acggaaaata
     2461 atatccggga tgcccagcta accccaaccg gtgtggatac caattatgtc cac
//
LOCUS       DAOQXT010000226         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_46618_length_2491_cov_3.583333, whole genome shotgun sequence.
ACCESSION   DAOQXT010000226 DAOQXT010000000
VERSION     DAOQXT010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46618_length_2491_cov_3.583333"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="istA"
                     /locus_tag="PKM27_19605"
     CDS             complement(<1..521)
                     /gene="istA"
                     /locus_tag="PKM27_19605"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNR09533.1"
                     /translation="MWEEYKREYPGGYQYSQFCYYLQLYDRSQQATFIRDHEPGDKIY
                     VDFTGDKLHYVDRNSGEIISCEAFVATLGYSNYTAVVAAHSQKIEEVVAAFGSVLEYI
                     GGSPRAVVPDNLKSAVHQAHRYEPRINEVFLDMANHYGMAVIPARPGKPKDKAKVERA
                     VAISYQRIFAPLR"
     gene            complement(2030..>2491)
                     /locus_tag="PKM27_19610"
     CDS             complement(2030..>2491)
                     /locus_tag="PKM27_19610"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HNR09534.1"
                     /translation="SPPRWDLSNQLADAEAAYAMILSRAAEWGVDPSRLGMIGFSAGA
                     GLTMHCTLQSKTMKLAFIGPIYGGMGPVEVPKDAPPMFNVIATDDFLFRGQFGVIDSW
                     FKAGRPVEFHLYQTGGHGFGLGNPNRTSNRWFEAFTHWLDVNGFLKAKAGK"
BASE COUNT          613 a          611 c          595 g          672 t
ORIGIN      
        1 ttccgtaagg gagcgaatat cctctggtag gaaatggcca cagcccgttc cacttttgct
       61 ttgtctttgg gtttcccggg tctggcggga atcacggcca ttccatagtg attggccata
      121 tccaggaaga cttcattaat ccttggttca taacggtgag cctggtgaac ggcagattta
      181 agattgtccg gcacaacagc ccgggggctt cctccaatat attccaatac agacccaaac
      241 gctgccacca cttcttcgat cttctgagaa tgggccgcta ctactgccgt ataattactg
      301 tatcccagcg tggctacaaa ggcttcacac gaaatgattt ccccactgtt acggtctaca
      361 taatgcaatt tgtcaccggt aaagtctacg taaatcttat ctcccggctc atgatcccga
      421 ataaaagttg cttgctggct tcgatcatag agctgcaaat aatagcaaaa ctgggagtat
      481 tgatatccac cgggatactc ccttttgtat tcttcccaca acagttgtta ggtgaggtga
      541 gggtgcctca gctcctggta aagtttatcc aggcgaagca tgaaatcctg atgtcgctct
      601 ctttcagtat ttaagggagc tcgcaatata tactcaatcc gaacatcctc aagctctaag
      661 aggtcctcca gatttaattc gcggcttttt atcgtccgga aatacccctt aatggtattg
      721 cgggaaatgc ctgtttcccg gacgattcct ttgatcggaa tgccctgcaa aaagaggcgt
      781 aggatttgtt tgagttgact catacgaatt gaatttcctg ccataatgtt gtgctttgtt
      841 tgagcaaagc acgcttgaca ttattacaat ccaaattcca gtatgaaatc aaggggtcag
      901 tatcccggaa tgacacaaat ttgagggggg tcagtatccc ggaaccaata ccgtaaaggg
      961 gggtcagtat cccggaatga cttttctata ccagggggtc agtatcccgg aacaaggggt
     1021 cggtatccgc cggaataggg ggtcagcatg acccggaata tacaccttgt tttcttcctc
     1081 ttccaaaagc ctcagtccgg ctcttattac ttcgcttgcg tttttatatc ttccctgggt
     1141 gattcttttt tcaacaaaat cttcaaaata ctctcctaag gatacagatg tgtttcgtcc
     1201 catgacttta aaaagtttaa ataaaattac caaattttgg taatttatca aattcccaaa
     1261 acagggagta tcaaagatcc cgcctgtttt ccatgcacaa ttttctttgt ggtccttgtg
     1321 tctcctgtat aatttgcggg ggccgaagct aagcttcgtc cgaactaatc ccgtagggac
     1381 ttcattactg gtagcataaa attttaggat ttaatatcgt cccggatggg acgaaataac
     1441 ctgcccgtat gagctaccca tatgacgtgc ctaacggcac attgttggat tgcgattttt
     1501 gcaaaacgat atcattatat gtcttttggg ctgaattctg aatggggggc cgaagtccta
     1561 acctgggttt cccactacga cacaacgaag tgaagggcac gaaggaatta tttgtgtgct
     1621 ttatggcatt cgtggggccg aagtccgaac atgggcatcg gagatgttcc acgtttttac
     1681 tactcatcca aagtaaaaac ccggcttcgg agaagcccaa catcgcccat caatgatatt
     1741 atccccgtta atttattaat tgggactgca tttgttaggc ttctccgaag ccattgtatt
     1801 gtcttatttt ttaccaataa ggtcaggcat ctccgatgct ggtaaatccc gggctgccag
     1861 gcagacttcc atgtctcgtt tatcagaaaa tgtaaaactg ctccagcagg cactggattg
     1921 tcataaaccc aaaacaggga atcacaaaga acccgcctgt tttccctgca cttttttctt
     1981 ggtggtcctt gtgtcttcgt gggagccctt gatgggaact ctgcgggacc tattttcccg
     2041 ccttagcctt caaaaatcca ttcacatcca gccagtgggt aaaggcctcg aaccaccggt
     2101 tgctggtccg gttgggattg ccgaggccga agccgtggcc tccggtctgg taaaggtgga
     2161 actcgacggg ccggcccgct ttgaaccagg aatcgatcac cccaaactgg ccgcggaaga
     2221 gaaaatcgtc ggtggcgatc acattgaaca taggcggagc atccttcggc acctcgaccg
     2281 ggcccatgcc gccatagatc gggccgatga aggccagttt catggttttg gattggaggg
     2341 tgcagtgcat ggtgaggccg gcgccggccg aaaagccgat catgcccagg cgggaaggat
     2401 caacccccca ttccgcggcc cggctgagga tcatggcata ggcagcctcc gcatcggcga
     2461 gctggttgga aaggtcccaa cggggcggac t
//
LOCUS       DAOQXT010000227         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_48098_length_2443_cov_5.221524, whole genome shotgun sequence.
ACCESSION   DAOQXT010000227 DAOQXT010000000
VERSION     DAOQXT010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48098_length_2443_cov_5.221524"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKM27_19615"
     CDS             <1..375
                     /locus_tag="PKM27_19615"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNR09535.1"
                     /translation="YTKAGKITIVSFWATWCGPCKKELDAYNAHYADWAKKYGTQLLA
                     ISIDDIRGQAKIKPMVSERKWPYTILVDANNDLQRQLGFNSIPQLYLLDKKGNIVHQA
                     TGYFPGSEKELEDKMAALAKSL"
     gene            complement(466..1614)
                     /locus_tag="PKM27_19620"
     CDS             complement(466..1614)
                     /locus_tag="PKM27_19620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR09536.1"
                     /translation="MDMHAHFFYEQGDHRNTNEKELKTMLANGLTTVRIMAGHPSYLE
                     AREKVSRGEWNGPKLYVASPQMVGRWPWSPKFENFGLVTTPEEARDKVFRFKKEGYDE
                     IKITFMVKKEVYDAIFEAAREAGIKVTGHVGPMVKLPWALKQKGQIEHMDEFIDMLLP
                     DSSYNHGESVSDMNLWRQNAWATVPHLMEDKIPSVAQEVKKAGIYVTPTNYFFLSCFG
                     SEQTEQYIKYKPDYPYIPEELLAERWQIRQRRLDMRIPESSLEKYRRIRHRLVGELWK
                     AGVPLMAGSDSPEWFLVPGFSIHDELESFVKSGLSPYAALQTATVNTSRYLGTPGSKG
                     MIDKDFDADLILLDGNPLEDIRNTRSIHGVLKEGKWYDRKTLDEFLGK"
     gene            complement(1614..1850)
                     /locus_tag="PKM27_19625"
     CDS             complement(1614..1850)
                     /locus_tag="PKM27_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09537.1"
                     /translation="MKRIGIVVFYLLSGLIARAQDLTVIKEVNVISMKDSGVLRNKSV
                     WIERGKITGINNRVPGNNRVCRVIDGKGKYLIRA"
BASE COUNT          621 a          677 c          522 g          623 t
ORIGIN      
        1 tataccaaag cgggtaaaat caccatcgtc agcttctggg caacctggtg cggcccctgt
       61 aaaaaggaac tggatgccta caatgcccat tatgccgact gggcgaagaa atacggcacc
      121 caactgcttg ccatcagcat cgacgacatt cgcggccagg ccaagatcaa acctatggtc
      181 agcgaacgga aatggcctta taccatcctg gtcgatgcca acaatgactt gcagcgtcaa
      241 ctcggattca acagcattcc ccagttatac ctcctcgata aaaaaggaaa cattgtgcac
      301 caggccaccg ggtatttccc cggaagcgag aaagaactgg aggataaaat ggcggctttg
      361 gctaaaagcc tgtaaaagct ctgatgagcc gcttcaaacc ggcattccgc agtttcattt
      421 gcaatcttaa aaacagggta aaaaagtccg atgaattttc cgggtttatt tacccaggaa
      481 ctcatccagg gtcttccggt cgtaccattt cccttccttc agcaccccgt gaatggagcg
      541 ggtattccgg atatcttcca gggggttccc gtccaggagg attaggtctg catcaaaatc
      601 cttatcaatc atgccttttg agccaggggt accgagataa cgcgaagtgt tgaccgtcgc
      661 ggtctgcaag gctgcataag ggctcagccc ggactttaca aaactttcga gttcatcgtg
      721 aatcgaaaat ccgggaacca aaaaccactc cggggaatcc gaaccggcca tcaggggtac
      781 accggccttc cagagttcgc ccaccaggcg gtgcctgatc ctccggtatt tttccaggct
      841 gctttccgga atcctcatat ccagcctgcg ttgcctgatc tgccagcgct cggcaagcag
      901 ttcctccggg atgtatgggt agtcgggctt gtattttatg tattgttccg tctgttcact
      961 tccgaagcag gaaagaaaaa aatagttggt cggagtcaca taaattcctg ccttttttac
     1021 ctcctgggct acggaaggaa ttttatcctc cattaaatgg ggaacggtcg cccaggcatt
     1081 ttgcctccaa aggttcatat ccgaaacgct ctctccatgg ttgtaactgc tgtccggcag
     1141 aagcatatcg ataaactcat ccatatgctc gatctgccct ttttgtttca gggcccaggg
     1201 cagcttgacc atcggcccca cgtgaccggt taccttgatg cccgcttccc tggccgcttc
     1261 aaaaatggca tcgtagactt cctttttgac catgaaagtg atcttgatct catcgtatcc
     1321 ctccttttta aaacggaaca ctttatcccg ggcttcctcc ggggtagtga ccaggccgaa
     1381 gttttcaaac ttgggtgacc agggccagcg cccaaccatc tgcgggctgg ctacatagag
     1441 ttttggacca ttccattctc ccctggagac cttctccctt gcttcaagat aggaagggtg
     1501 acccgccatg atgcgcaccg tcgtcaggcc gttggccagc atggttttta attccttttc
     1561 attggtattc cggtggtcgc cctgctcata gaaaaaatga gcatgcatgt ccatcaggcc
     1621 cgtatgaggt atttgccctt gccgtcgatg acccggcaaa cgcggttgtt ccccggaacc
     1681 cgattgttga tccccgtgat cttgcccctt tcaatccata ccgatttatt ccttaaaacc
     1741 ccggagtcct tcatgctgat gacattgacc tccttaatga cggtcaggtc ctgagcgcgg
     1801 gcgatcaaac ctgaaagcaa gtaaaaaaca acaatcccga ttcttttcat aaaatgaaga
     1861 tcttttcagg aacgaaatta gctgttgctg tcggttcggt tttcacccgg atagggtgat
     1921 ttaatccaga atggtttaag cgtataattt tgcagaacgt ccttcttacc tcttacctct
     1981 gacctctgac ctctgacctc tgacctctga cctcttacct caaacaaaaa agcccatcag
     2041 cattcaataa ccattcactg accgggcaaa taaaaaaccc ctgtggggca aattaaaaat
     2101 cttattcttt tcttttaatg aaacaaagat gccgggctta tgtaaactat atattaaggc
     2161 tgtattatgc ctgtattaaa cccaaaccat atctaaaaag gttaaccttt gcctgctgga
     2221 aagaaagggt cagcccccaa gaactccatt accctcgtgt gccaaaaact caatctccat
     2281 taactccgtt actccgtgcg ccaaaaaact caatctcccc aaaaactcca tttctccgtg
     2341 cactaaaact cattctccca aaaaccccac cacctccgac ctcaataact ccggttgatc
     2401 cgcatggatc caatgccccg ccccgggtat ggttttaaaa gaa
//
LOCUS       DAOQXT010000228         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_51933_length_2325_cov_6.626432, whole genome shotgun sequence.
ACCESSION   DAOQXT010000228 DAOQXT010000000
VERSION     DAOQXT010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51933_length_2325_cov_6.626432"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKM27_19630"
     CDS             <1..359
                     /locus_tag="PKM27_19630"
                     /inference="COORDINATES: protein motif:HMM:NF016165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HNR09538.1"
                     /translation="LENALVQLKNSAEEFQKSSGAALSLSPDKQKELNQLLYQIEKSL
                     LQPEGLPRRPWYKHQIYAPGYYTGYGVKTMPGVREGIEQRNFREAQQNIEILGKTLTG
                     YADEINKTLTLLKAKS"
     gene            389..1903
                     /locus_tag="PKM27_19635"
     CDS             389..1903
                     /locus_tag="PKM27_19635"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR09539.1"
                     /translation="MKKKKSINRRQFIGKASTASLSAMAFHIVPRHTLGGPGFLAPSD
                     KLNLAYIGCGTQGLREMCQLITNPKLRITTVCDPNKLSTNYLDWSAHGIRNGIRKVLG
                     DDSWGEHFKGIPGGRDIGKELVDKYYNKVNNTSDNNCSSYEDFRELLEKEKDIDAVKI
                     MTPDHLHGYISVAAMKKGKHVVIHKPLANRMHEARLVIQTAKETGVSSHLLAWSRRTG
                     LETINQWISQGRIGTLKEIHNWSNRPQWPQWTSNPKDSPPLPEGFNWDLWLGCVPDRP
                     YHPNYTHNVFRGWYDFGGGSIADMGHYSLWPLFLTIGIETAPYSVEAMGTTTCTVEDQ
                     VCKTVQNDVAFPYSCSIRFRFPSQSRLPAFDLYWYDGGIRPATPDELGDGVLEREGML
                     IVGDQGKILAGFRGERPRLLPEEKMKLAANGQPAPTESTTRGDDVWLDAFKNKVQSPG
                     SFLYAGPVTETIQLGAVALRARKKLLYDSAAMKITNNEDANKYFYREYRKGWEM"
     gene            1991..>2325
                     /locus_tag="PKM27_19640"
     CDS             1991..>2325
                     /locus_tag="PKM27_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09540.1"
                     /translation="MKRRTFLETTALGAGSFALTGNPLTYQSPITNHHSPMKDDISLA
                     QWALVEEMRAGKWKNLDFPRIAREDFGLNGIEFVNTLFEVPTEGYLKKLKKNAADQGV
                     TMVLIMVDD"
BASE COUNT          635 a          634 c          583 g          473 t
ORIGIN      
        1 acctggaaaa tgccctggtc caattaaaaa acagcgccga agaatttcag aaatcatccg
       61 gtgccgccct gagcctttca ccggataaac aaaaagaact gaatcaattg ctgtaccaga
      121 tagaaaaaag ccttctacaa ccggaaggcc ttccccgccg gccctggtac aagcaccaga
      181 tctatgcacc gggctattac acgggttatg gggtcaagac tatgccgggc gtgcgggagg
      241 gcatcgagca aaggaatttc cgggaagccc agcaaaatat tgaaatcctg ggaaagaccc
      301 tgaccggata cgccgatgaa atcaataaaa ccctaacttt attaaaagca aaatcctgac
      361 ccatttaatt catccctatt tatcaaatat gaaaaaaaag aaatccatca accgccggca
      421 atttataggc aaagcctcca ccgcatccct cagcgccatg gcttttcata tcgtgccccg
      481 gcataccctt ggaggacccg gattcctggc gcccagcgat aaactcaacc tggcttatat
      541 cggttgcggt acacagggcc tgagggaaat gtgccaactg atcaccaatc ccaaactccg
      601 gatcactacg gtctgcgatc ccaataaatt aagcaccaat tacctcgact ggtcggcgca
      661 cgggattcgg aacgggatcc gcaaagtatt gggggatgat tcctggggtg agcattttaa
      721 aggcattcct ggtggccggg atatcggcaa agaattggtg gacaaatatt ataacaaggt
      781 caataacacc agcgataaca actgttcttc ttatgaagac ttcagggaac tgcttgaaaa
      841 agaaaaagat atcgatgcgg tcaagatcat gactcccgat cacctgcacg gttatatttc
      901 cgtggcggcg atgaaaaaag gcaaacacgt agtgatccac aagcccctcg ccaaccggat
      961 gcacgaggcc cgcctggtga tccagaccgc caaagagacc ggggtcagca gccacctcct
     1021 ggcctggtcc cgaaggaccg gcctcgaaac catcaatcaa tggatcagcc agggccggat
     1081 cggtaccctg aaagaaatcc acaactggtc caaccggccc caatggcccc aatggacttc
     1141 caatccaaag gacagtcctc ccctgcccga gggtttcaac tgggatctct ggctgggatg
     1201 cgtaccggac cggccctatc atcccaatta tacccataat gtattccgcg gatggtatga
     1261 tttcggaggg ggcagtatcg cggatatggg acattacagc ctttggccct tgtttttgac
     1321 cattggtatc gagaccgccc cctattccgt ggaggccatg ggcacaacga cctgcactgt
     1381 ggaggatcag gtgtgcaaaa cggtacagaa tgatgtggcc ttcccctatt cctgttccat
     1441 tcggttcagg ttcccttccc aaagccgttt gccggccttt gacctttatt ggtatgacgg
     1501 cggcatccgg cccgccaccc ccgatgaact gggtgatggc gtcctggaaa gggaaggtat
     1561 gctgatcgta ggcgaccagg gcaagatcct ggccggattc cggggggagc ggccccgtct
     1621 cctgccggaa gaaaaaatga aattagccgc caatggccag cccgctccaa cggaatcaac
     1681 cacccggggt gatgacgtat ggctcgatgc atttaaaaat aaagtccagt ccccagggag
     1741 ttttctttac gccggcccgg tgacagagac gatccagctc ggcgccgtcg ccctgcgcgc
     1801 caggaaaaaa ctgttatatg attccgccgc gatgaaaatc acaaataatg aggacgcaaa
     1861 taagtatttt tacagggagt acaggaaagg atgggaaatg tgagtggcga gcagcgagaa
     1921 tcgagctgcg agttgcgagt tgcgagccgc atccccaaac ttccagcttc caacttctaa
     1981 ctcctaaaca atgaaaagaa gaactttttt agaaaccacc gccctgggcg ccggatcttt
     2041 tgcactgacc ggcaatccat tgacttacca atcaccaatc accaaccacc actcacccat
     2101 gaaagacgat atttccctgg cccaatgggc cctggtcgaa gaaatgcgcg ccggaaaatg
     2161 gaaaaacctg gattttcccc gcattgcccg ggaggatttt gggctcaacg gcatcgagtt
     2221 tgtgaatacc ttgtttgagg tccccaccga aggctatctg aaaaagctga agaaaaacgc
     2281 cgccgaccag ggggtcacca tggtgctgat catggtggac gatga
//
LOCUS       DAOQXT010000229         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_53969_length_2267_cov_7.091320, whole genome shotgun sequence.
ACCESSION   DAOQXT010000229 DAOQXT010000000
VERSION     DAOQXT010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53969_length_2267_cov_7.091320"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKM27_19645"
     CDS             complement(<1..696)
                     /locus_tag="PKM27_19645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR09541.1"
                     /translation="MQEKIPELEKKIEFFQAIADNTYDWEIFRDANNKLIYCSPAIEN
                     LLGYTADEYLQGLPQEQIFHAEDLPKAIHDFNRALKGETVPSCIVRMITKEKRIIWVE
                     IASKPVYSKSGEFIGFRSSVRNISHLKKIEQELNESKEMLKLAFHYSNDWELYRDKNM
                     KPVYCSQSIEKIVGYTPEEYLSEKVKPKDVIHPEDWPKFQTIYDRILNGETVNSLILR
                     YLHKNGHLVWVDLS"
     gene            930..1757
                     /locus_tag="PKM27_19650"
     CDS             930..1757
                     /locus_tag="PKM27_19650"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNR09542.1"
                     /translation="MKKISLFGLVFFMAFSLTAQDKVGVQLYSFRNQFKTDVPGTLKA
                     IREMGITYVEGGDSYGLPIKDIKMLLAENKFKVVSVGCDYNELKTDPMSVVEKAKAYG
                     ASFAMCPWIPHPEGPLTIELAKEAVMVFNTAGKVLKDNGIDLVYHAHGYEFSPYEDGT
                     VFDYMAKNMDPKVANFEMDVYWISHPGQDPVKLLQKYPGRFLLMHLKDRKPGTPGNML
                     GRADVETNVVLGKGDLGIEAIFKAGRKAGVKYYFIEDESSRSMEQVPQSLAYLKQIK"
     gene            1838..2209
                     /locus_tag="PKM27_19655"
     CDS             1838..2209
                     /locus_tag="PKM27_19655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR09543.1"
                     /translation="MSTLERINNFKQRTKNLALKTIKLYQSLPKRDETLIVGKQLLRS
                     STSVAANYRAACRARSGPEFFSKISIVVEEADETLFWLEILQESGISGGTDVNLLMNE
                     YLEVLKIVNTIRHNRKVRSEE"
BASE COUNT          587 a          497 c          594 g          589 t
ORIGIN      
        1 cgagaggtcg acccatacga gatgcccatt cttatgcagg taccggagga tcagggaatt
       61 tacggtttcc ccgtttaaga tccggtcgta gatcgtttgg aatttgggcc agtcctcggg
      121 atggatcacg tctttgggtt taaccttttc ggaaaggtat tcttcaggcg tatatccgac
      181 gatcttttcg atggattggc tgcaatagac cggcttcatg tttttatcgc ggtacagctc
      241 ccagtcgttt gaataatgaa aggccagttt cagcatttcc ttgctctcgt tcagttcctg
      301 ctcgatcttc ttcaggtggg atatgttgcg gacactggac cggaagccga taaattcccc
      361 ggatttggag taaaccggtt tggaggcgat ttcgacccag atgatccgtt tttctttggt
      421 gatcatgcgc acgatgcacg agggaaccgt ttcccccttc aaggcccggt taaaatcatg
      481 gatcgctttg ggcaggtcct ccgcatgaaa gatttgttcc tggggaagtc cctgcaggta
      541 ttcgtcggcg gtatatccta acaggttttc aatggctgga ctgcaataaa tcagtttgtt
      601 gtttgcatcc ctgaatattt cccaatcata agtattatcc gcgatggcct ggaaaaactc
      661 aatttttttc tcgagttctg gtattttctc ctgcatcttg acggcattta atggatctcc
      721 ggggagccgt atccagacac atggttttga ttgatcgaat ggggtgacag ctccaattcc
      781 tcgggtaata tatcaggcta agttaggaaa tcaatagttc tctggccaat ttttcattgg
      841 ccaggcttag gcttgtttgc aagtaaaata tttaccatct aatccgccat ttctcacaac
      901 tttgcattga aactaaaaac aagagtataa tgaaaaaaat atccttattc ggcctggtct
      961 ttttcatggc tttcagcctg accgcccagg acaaggtcgg cgtgcagctt tacagtttcc
     1021 ggaaccagtt taagacggat gtgcccggca cgctcaaagc catccgggaa atgggcatca
     1081 cctacgtgga agggggcgac agctacggcc tgcccatcaa agacatcaag atgctcctgg
     1141 cggaaaacaa attcaaggtc gtcagcgtgg gttgcgatta caacgaactg aagaccgatc
     1201 ccatgtccgt ggtggaaaaa gccaaagcct acggcgcttc ctttgccatg tgtccctgga
     1261 tcccgcatcc cgaaggcccg ctcaccatcg aactcgccaa agaagccgtc atggtcttca
     1321 ataccgccgg caaggtgctc aaggacaacg gcatcgacct ggtctaccat gcccatgggt
     1381 atgaattttc accctatgag gacggcactg tattcgacta catggccaaa aacatggatc
     1441 ctaaagtggc caatttcgaa atggatgtgt attggataag ccatcccggc caggacccgg
     1501 tcaaattgct acagaaatat ccgggccgtt tcctgctgat gcacctgaaa gaccgcaaac
     1561 ccggcactcc gggcaatatg ctcggccgcg ctgatgtgga aaccaatgtg gtcctgggta
     1621 agggcgacct cggcatcgaa gccatcttca aagcgggccg taaagccggc gtgaaatact
     1681 attttatcga agatgaatct tcacgctcaa tggagcaggt gccgcagagt ctggcgtacc
     1741 tcaagcagat aaaataggat tgaggattga ggattgagga gtgaggggtg aggattgggg
     1801 gtgagggttg aggattgaga aataacacac ttaatatatg agcacacttg aacggatcaa
     1861 taattttaaa caacggacta agaatttagc gcttaagacc ataaaactat atcagtctct
     1921 ccctaaaagg gacgagacac taattgttgg gaaacaactt ttgcggtctt caacttctgt
     1981 agcagcaaat tacagggctg cctgccgtgc acgttcaggt ccggagtttt ttagtaagat
     2041 ttcgattgtt gtggaagaag cggatgaaac tcttttttgg ttggaaatcc tgcaagagtc
     2101 tggtatttcc gggggtacgg atgtaaactt attaatgaac gaatacctgg aagttttgaa
     2161 aattgtcaat acaatcagac ataataggaa ggtgaggagt gaggagtgag gagtgaggag
     2221 tgaggagtga ggagtgagga gtgaggagtg aggagtgagg agtgagg
//
LOCUS       DAOQXT010000230         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_54358_length_2257_cov_4.811081, whole genome shotgun sequence.
ACCESSION   DAOQXT010000230 DAOQXT010000000
VERSION     DAOQXT010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54358_length_2257_cov_4.811081"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..>1066)
                     /locus_tag="PKM27_19660"
     CDS             complement(32..>1066)
                     /locus_tag="PKM27_19660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR09544.1"
                     /translation="YVPSYGPVSNPNPDLKWEKKGETNVGLEFGIWDYKLTGSIDVFN
                     RKVNDLLYNFPVPVPPNPFGRTLANVGELTSSGLEALVNLNDLKFGQLKYTPSLNATF
                     YLNNELVSLSEGDLKFGSGGILDLAGVGSPGQNNYTMIRVAEGEKIGQIYGPVFDGVD
                     ENGNPKFKDLNKDGKVESAGADREVLGNGLPNLTLGLNNTLSLGDWSLNFFLRGAFGH
                     SLVNQYRVFYEDYNAGSIKNYNRILTKLSDTKIKNAQYSSIHVEKADFLRLDNMQLGY
                     SLPVKGAFSKAFVYVASNNLFTISGYTGIDPEVRFVDDEDGNPLAPGIDRRTNYFTSR
                     SFSLGLNLGF"
     assembly_gap    1067..1166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1167..>2257)
                     /locus_tag="PKM27_19665"
     CDS             complement(<1167..>2257)
                     /locus_tag="PKM27_19665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNR09545.1"
                     /translation="SITSPAQLLQGKVPGLIIAKPNGDPNGGFNIRLRGLSTVGGNTE
                     PLIIIDGVPGASLNTVDPMDIETMDVLRDGSAAAIYGTRGSSGVILITTKKGKEGRTV
                     VDYNGIASVESLAKAPSVMTSDEYKKAGGIDQGSSTDWFDVITRSALQQQHNLSLSGG
                     ARGMGYRVSFNYKDIQGIQLNTGFKQLNGRVSLNQKALNDKLNLGFDLAATNRKQDIN
                     FRDAFRYATYFNPTAPVYNAANTVNGGYFEPGGFDYFNPLAIVEQNSNTGEFTNVLAT
                     IRGEYEFIKDLKLNVAYTYGRDNGHSQQYFSKQSQYGTGLGRNGLATQNTGFNLNDYL
                     STTLKYNFNTIGVDWSVLGGYDYQKFTGEG"
BASE COUNT          488 a          542 c          564 g          563 t
ORIGIN      
        1 tgttcatatc gctatgattt atattttttt attaaaaacc aaggtttaag cccagcgaga
       61 aagaacggct ggtaaaataa tttgttctgc ggtcaatacc cggggcgagc gggttgccat
      121 cctcatcatc cacaaaacgg acttccggat cgatcccggt atacccggat atggtaaaca
      181 ggttgttgct ggctacgtac acaaaggctt ttgaaaaggc cccttttacc ggcaggctgt
      241 aacccaactg catgttgtcc agccgcagga aatcggcctt ctccacgtgg atgcttgaat
      301 actgggcgtt tttgatcttc gtatcggaaa gtttggtaag gatccggttg tagttcttaa
      361 tggagcccgc attgtaatct tcatagaaaa cccggtactg gttgaccagg ctatgcccga
      421 atgcccctct caggaagaag ttcagggacc agtcgcccag gctcagggta ttgttaagac
      481 ccagggttag gttgggtaag ccattgccca atacttcccg gtcagccccg gcggattcca
      541 ccttgccatc cttgttgagg tccttgaatt tcgggtttcc gttttcatcc actccatcaa
      601 aaacgggacc atagatctgg ccgatttttt cgccctcggc tacccggatc atggtatagt
      661 tgttttgtcc gggagaacct actccggcca ggtcaagaat ccctcctgag ccgaatttga
      721 ggtctccttc ggaaagactg accagttcgt tattcagata gaaagttgcg ttcaggcttg
      781 gggtatattt caactggcca aatttgaggt cgttcaggtt gaccagggct tccaggccag
      841 agctcgtcaa ttccccaaca ttggccaagg tgcgcccaaa cggattcggg ggcacgggga
      901 ccgggaaatt ataaaggagg tcattgactt tacggttgaa cacgtcgatg gaaccggtca
      961 acttataatc ccagattcca aactcgaggc ccacattggt ctcgcccttt ttctcccatt
     1021 tcaggtcggg attggggttg gataccggtc cataagaagg tacatannnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnncctt ccccggtgaa tttctgataa tcgtatccgc
     1201 cgaggaccga ccaatccact ccaatggtat taaaattgta cttcagggtg gtggacaggt
     1261 aatcattcag gttaaaaccg gtattctggg tagccagacc gttgcggccc aaaccggtac
     1321 cgtactggct ttgcttgctg aaatactgtt ggctgtgccc attgtcccgg ccgtaggtat
     1381 aggccacatt cagtttgagg tccttgataa actcatattc ccccctgatg gtggccagga
     1441 cattggtgaa ttcaccggtg ttggaatttt gttcgacgat ggccagggga ttaaaatagt
     1501 caaatccgcc gggttcaaaa tagccgccat tgaccgtatt ggcagcattg tatacagggg
     1561 cagtgggatt aaaataggtg gcataacgaa aggcatcccg gaaattaatg tcctgtttac
     1621 ggtttgtggc cgccaggtca aagcctaaat tgagtttatc gttaagggcc ttttgattca
     1681 ggctgaccct gccgttgagt tgtttaaacc cggtattcaa ctggatgccc tggatgtcct
     1741 tgtagttaaa ggaaacccgg tatcccatac cccttgctcc gccggaaagg gagaggttgt
     1801 gttgttgctg gagagcgctc cgggtgatca cgtcaaacca gtcggtactg ctgccctggt
     1861 ctataccacc ggctttttta tattcatcgg aggtcatcac gctcggcgct ttggcgaggg
     1921 attctaccga ggcaatgccg ttgtaatcaa ccacggtcct gccttccttg ccttttttgg
     1981 tagtgatcag aatcacccca ctggaaccac gggtaccata aatggccgcc gccgaaccat
     2041 cccgcagcac gtccatggtt tcgatatcca taggatccac ggtattgagg ctggcgcccg
     2101 gtaccccgtc aatgatgatc aggggttcgg tattcccgcc caccgttgac aaaccgcgaa
     2161 ggcgaatgtt aaatccaccg ttggggtccc cattgggttt ggcaatgatc aggccgggga
     2221 ccttgccctg gagcagctgg gccgggctgg tgatcga
//
LOCUS       DAOQXT010000231         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_59103_length_2141_cov_5.973154, whole genome shotgun sequence.
ACCESSION   DAOQXT010000231 DAOQXT010000000
VERSION     DAOQXT010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59103_length_2141_cov_5.973154"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            581..1909
                     /locus_tag="PKM27_19670"
     CDS             581..1909
                     /locus_tag="PKM27_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HNR09546.1"
                     /translation="MPKFISFRQGSSSVLFWSVISAAFIGPGTVTTCSKAGASFEMSL
                     LWALLFSTLATILLQELAARVNMATGKNLGEIMSIKYQSKKASSRIPGFLFLAVSFGC
                     AAYQAGNILGAVSGLRLLSPFPSFAFTLVIGILCFILLWKGTVGQVARFMGALLVIMG
                     IIFLYIAVQVGPALGGLLKGSLLPTIPAGSGSLIIGLIGTTIVPYNLFLASGVQKKQS
                     LSSMRLGIAIAVGIGGLISMAILISGTLLSGEYSYALLSGAIQEHTGKMGSVFFALGL
                     FAAGFSSAITAPLAAAVCGQSFFRTASPESWSENGKYYKMTWIAVLLVGVLFGFMNVS
                     PVPAILAAQGINGLLLPLVCIAIYLATRDQGMMPMEYRHRPWLDKVYLFIIWVTCFLG
                     LYNIQKSTLNIFGIQGLTETKLILISASLGAAFVLLLNQDQERSRHQAGN"
     gene            complement(1886..>2141)
                     /locus_tag="PKM27_19675"
     CDS             complement(1886..>2141)
                     /locus_tag="PKM27_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09547.1"
                     /translation="PFLRKGFFDPAGTLPHSLFHVAHVREQCVRFLTDHLQGDLYYHQ
                     PFTGFNLKAVQVQQEFIRMLRRKRAWMEERWAGLVAGLVP"
BASE COUNT          485 a          558 c          511 g          587 t
ORIGIN      
        1 ggatgaagat tagcagcttt ttcatttctt tcctgaaagt gaaatctaaa ttacagacta
       61 ttctcatttt cccggttatt gttgcagggt agttgaatcc tatattgcag tttacagttc
      121 attaaagatg ataaataacg gcttgaaagg cccctatttc cccagcattg ggcaaacttg
      181 cccatgctgg ggataagaag aaatccaatc gccctgtaag ggcattattt tttcatgtac
      241 cctgccttgt ttataatccg gaaacatcaa tatgcatcag attgcttaaa ttgatatgaa
      301 tggtgccggg gatgatcttg taatgatgcc ctttcagggc gacccactcc gacattcccg
      361 agcaccgggc tattcgcccg atgctcggga aatgatgccc cttcagggca aattcatacg
      421 agtaagtgtc ttagatcatg ttaggattga ctgaatggcc cctgcaggcc aaattcatac
      481 aagaaagtgt ccattcttca tagaatacct tttgggtttg aaactttgaa tattccggaa
      541 caaccaaaga tcaatgatgc acacttacct ttgcccccga atgccaaaat tcatttcctt
      601 ccggcaagga tcttcctccg tgctcttctg gtccgtcatt tccgcggctt ttatcggccc
      661 gggcaccgtg acgacctgct ccaaggcagg cgcttctttt gaaatgtccc tgttatgggc
      721 cctcttgttt tcaacgttag cgaccatcct gctgcaggaa cttgccgcaa gggtcaatat
      781 ggctaccggt aagaaccttg gcgaaatcat gtccatcaag taccaaagta aaaaagcttc
      841 ctcccggata cctggatttt tattcctggc ggtctctttt ggatgcgccg cttaccaggc
      901 cggtaatatc cttggagcgg tgtccggact tagactgtta agccctttcc cctcctttgc
      961 tttcaccctg gtcatcggca tcctttgttt tatcttgttg tggaaaggca ctgtaggcca
     1021 ggtggcccga ttcatgggag ccctgttagt catcatgggg attatatttc tctacatcgc
     1081 agtacaagtt gggcctgcac tgggtggatt gctgaaaggc agcctccttc cgaccatccc
     1141 cgccgggagc ggaagcctga tcattggttt gatcggtacg accatcgtgc cttataattt
     1201 gttccttgca tccggagtgc agaaaaaaca aagcctttcc tcgatgcggt tgggcattgc
     1261 cattgccgta ggtattggcg ggctgatcag catggccatt cttatcagcg gcaccttatt
     1321 gtccggagaa tattcctatg ccttgctctc cggcgcgatt caggagcaca ccgggaaaat
     1381 gggttctgta tttttcgcgc tgggtttgtt tgccgcggga ttcagcagcg ccatcactgc
     1441 ccctttggcg gcagcggttt gtggccaatc tttctttcgg accgcaagtc ctgaaagctg
     1501 gtctgaaaac ggaaaatact acaagatgac ctggatcgcg gtcctgctgg ttggggtcct
     1561 cttcggtttt atgaatgtaa gtccggttcc ggccatcctc gctgcccagg gcatcaacgg
     1621 gctcctgctc cccctggtat gcatcgcgat atatcttgcc accagggacc agggcatgat
     1681 gcctatggaa taccggcacc ggccgtggtt ggataaggta tacctcttca tcatctgggt
     1741 cacttgtttt ctcggccttt acaatatcca gaaatcgact ctaaacattt ttgggataca
     1801 aggcctcacc gaaacaaaac tcatcctgat ctccgcctcc ctgggagcgg ccttcgtatt
     1861 attgctcaac caggaccagg aacggtcaag gcaccaggcc ggcaactaat ccggcccacc
     1921 gttcctccat ccatgctctt tttctccgga gcatccggat aaactcctgc tgaacctgca
     1981 ccgccttcag gttgaatccg gtaaatggct ggtgataata aagatctccc tgaagatggt
     2041 ccgtgaggaa acgcacacat tgctcccgga catgcgcaac atggaataag gaatgaggga
     2101 gagttcctgc cgggtcaaaa aatcctttgc gcaggaaagg t
//
LOCUS       DAOQXT010000232         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_61505_length_2088_cov_4.528283, whole genome shotgun sequence.
ACCESSION   DAOQXT010000232 DAOQXT010000000
VERSION     DAOQXT010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61505_length_2088_cov_4.528283"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKM27_19680"
     CDS             complement(<1..602)
                     /locus_tag="PKM27_19680"
                     /inference="COORDINATES: protein motif:HMM:NF037030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09548.1"
                     /translation="MGLKKWINILSRPLREARANRNSPIQFHNFWEFRSPSELWFYKF
                     IQHRNLLEKANLKEIHFYSVFRQVNTPFRERYFRNRENIFSIFYTGENVDNYPAFSDH
                     LLDKVDLSLGFEYIEHPRYLRFPIWIWFTIDPTWDLEKIKNQLDQQFENRRFQKRDGF
                     CCMVSSHDRNGMRYRLMKAVEKYGKVDSGGKKYNNTNALK"
     gene            complement(604..>2088)
                     /locus_tag="PKM27_19685"
     CDS             complement(604..>2088)
                     /locus_tag="PKM27_19685"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNR09549.1"
                     /translation="LSFTGSPDYMGAIICLLFLFGLFWVKGPVKWGMAAATILLVLMS
                     LGKNFELLNRFLFENLPLLNKFRTPNSIHNVTSCLIPLFAIYTLWQVIDLDKGRKAVL
                     GLFVKVLAGFVGFVLLFGIGGGLFFDFTAAGDARYEPTLVNVLKEARAIYLRQDAFRT
                     LFFVLAGGILLYLFLREKLKTGYLLAGLTILTFLDLFGVARRYLSPDDWQAKTSITQG
                     NPKRPVDQQILEDPQLYYRVLDLGGDPFQNASPSFYHKSVGGYHPAKLRRYQDIIDAY
                     LVKGDQKMLNMLNTKYIITQDQQAQLNAGAMGNAWLVGDIRKVATPDEEIAAIRDIDP
                     AQTAIVLDQEFPGYLTNGSFSRQGSITLTKYEPNALTYASQVEGDQFAVFSDIWYGPD
                     KGWQAYVDGQPADHIRVNYVFRGMKIPSGNHTIEFKFIPVKVLRSIKLGFWLNNLAGL
                     LLLGLCGWLFYSWYKTEASSPVAAPQAQPVVKPGKPGIKKGKKP"
BASE COUNT          553 a          512 c          494 g          529 t
ORIGIN      
        1 tgtttcaggg cattggtatt gttgtatttt tttcctccgc tgtccacctt tccatatttt
       61 tcgaccgctt tcatcaaccg gtatctcatt ccattacggt cgtgactgga aaccatacaa
      121 caaaatccat ctcttttttg gaaccgtctg ttttcaaact gctgatccaa ttggtttttg
      181 atcttttcca aatcccaggt tggatctatc gtaaaccaga tccatattgg aaaccgaagg
      241 tatcgcggat gttcaatata ttcaaatccc aaagacaagt ctaccttgtc cagcaaatga
      301 tcgctgaacg cgggataatt gtccacattt tcacccgtat aaaagatgga aaaaatgttt
      361 tcccgattgc gaaaatacct ttcccggaaa ggagtattta cctgcctgaa cacagaatag
      421 aaatggatct ccttcaaatt ggctttttcg agcaaattcc ggtgttgaat aaacttataa
      481 aaccaaagtt ctgacgggga acggaattcc caaaaattat gaaactggat aggagaattt
      541 cgattggccc gcgcctcccg cagaggccgg gaaagtatat tgatccactt ttttaagccc
      601 attttatggc ttctttccct ttttaatccc gggtttgccc ggcttgacaa caggctgcgc
      661 ttgcggagcc gcaaccggtg aagaggcttc cgttttgtac cacgaataaa acaaccaccc
      721 acacaatcca agcagcagca gtcctgccag attattcaac caaaagccca gtttgatgga
      781 acgcagcacc ttaaccggga tgaatttaaa ttcaatggta tggttacctg aaggtatttt
      841 cataccccgg aaaacataat tgacccggat atgatcggcc ggctggccgt ccacataggc
      901 ctgccatccc ttgtccgggc cataccagat atcggaaaat acggcaaact ggtctccttc
      961 cacctgggag gcataagtca gcgcattggg ctcatatttt gtaagggtta tgctgccttg
     1021 ccttgaaaac gagccgttgg tgaggtaccc cgggaattcc tggtccagca caatcgcagt
     1081 ctgggccgga tcgatatccc ggatcgcggc aatttcttca tcaggtgtgg ccaccttgcg
     1141 gatatcgccc accagccagg cattgcccat ggccccggca ttcaactggg cctgctggtc
     1201 ctgggtgatg atgtatttgg tattgagcat attgagcatc ttctggtccc ctttgaccag
     1261 gtaggcgtca atgatgtcct ggtatctccg gagtttggcg ggatggtagc cccccacgga
     1321 tttatgataa aaggagggcg atgcgttctg aaaagggtcg ccgcccaggt cgaggacccg
     1381 gtaatagagt tgcgggtctt ccaggatctg ctgatccaca ggccgttttg gatttccttg
     1441 tgtaatggag gttttggcct gccaatcatc cgggctgagg tatctgcggg ctaccccgaa
     1501 aaggtccagg aaagtgagaa tggtaaggcc ggcaagcagg taacctgttt taagtttttc
     1561 tctcaaaaat aaatagagga gaattcctcc ggccaggaca aaaaacaggg tcctgaaggc
     1621 gtcctggcga agataaatgg ccctcgcttc cttgaggaca ttgaccaggg tgggttcata
     1681 ccgggcatcc cctgccgcag tgaaatcaaa aaacaaacct cctccaattc caaacagtaa
     1741 tacaaaacca acaaaacccg caagcacttt tacaaaaaga ccgaggactg cttttctgcc
     1801 tttgtcgagg tcaatcactt gccaaagggt ataaatggca aagaggggga tgaggcaact
     1861 cgtaacgttg tggatggaat tgggtgtcct gaatttattg agcaggggca gattttcgaa
     1921 aaggaaccgg ttgaggagtt caaaattttt acccagggac attaatacca ggagtatcgt
     1981 cgcggctgcc attccccact tcaccggccc tttaacccaa aaaaggccga acaggaaaag
     2041 caaacagatg atggctccca tataatccgg cgaccccgta aaactcag
//
LOCUS       DAOQXT010000233         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_62960_length_2058_cov_6.601098, whole genome shotgun sequence.
ACCESSION   DAOQXT010000233 DAOQXT010000000
VERSION     DAOQXT010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62960_length_2058_cov_6.601098"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1824
                     /locus_tag="PKM27_19690"
     CDS             118..1824
                     /locus_tag="PKM27_19690"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HNR09550.1"
                     /translation="MELRSRNWFGKTGKDGFIYRAWMKNQGIPPEEFKGKPVIGICNT
                     WSELTPCNAHFRELAESIKHGVIQAGGFPVEFPVMSLGETLIKPTAMLYRNLVSMDVE
                     ESIRANPVDGVVLMCGCDKTTPSLVMGACSVNIPTLVVSGGPMLAGKFRGRDIGTSDI
                     WRFGEAYKLGQMDDQDLEEAESAMCRSRGHCAVMGTASTMACMVESLGLSLPGNASIP
                     AADARRKVLAQRSGLRIVEMVKENLQLSDVLTKKAFENAIRVNAAIGGSTNFVLHMIA
                     IAGRIGVELKLEDFDRCSKGIPLLANLQPSGEFFVEDLYYAGGLPAVMKEMESFLHGD
                     ALTVNGKTVHENISSAKCYNPAVIGTLDKPFKTETGLAILRGNLCEGGCVIKPSAASS
                     HLMKHRGRAVVFEDIEDYKKRIDLPDLDVDETCILVLKNVGPKGYPGMPEVGNMALPK
                     KILQKGIHDMVRISDGRMSGTGFGTVVLHASPEAAAGGNFAVVKTGDYISLDVDARSL
                     MLEVSEEELALRKASWIAPKPLADRGYVSLYIRHVNQAHEGADLDFLVGNSSSVVTRD
                     SH"
BASE COUNT          519 a          486 c          588 g          465 t
ORIGIN      
        1 cctggcccgg tccaggaaat tctggaagga attattgtcc tgaacgtgtg caccggtata
       61 tccggtaccc ggcaaaatga ttagtttagt cccctaaaat tcacaatagt aaaaagtatg
      121 gaattgcgca gcagaaattg gtttggaaaa accggaaaag atgggtttat ctaccgggcc
      181 tggatgaaaa accagggcat tcctccggaa gaatttaaag gcaaaccggt gatcgggatt
      241 tgtaatacct ggtcggaact aaccccctgc aacgcgcatt tcagggaact tgccgaatcc
      301 atcaaacatg gggtaatcca ggcagggggt ttcccggtgg aattcccggt catgtcgctg
      361 ggcgagaccc tgatcaagcc tactgcgatg ttataccgga acctggtgag tatggacgtc
      421 gaggagtcca tccgcgccaa cccagtggat ggggtcgtat tgatgtgtgg ctgcgacaag
      481 accaccccct cgctcgtgat gggggcctgc agcgtgaaca tacccaccct cgtagtcagt
      541 ggtggcccga tgctggccgg taaattcagg ggcagggata tcggcacctc ggacatctgg
      601 cgtttcggtg aagcttacaa attgggacaa atggacgacc aggacctgga ggaagcggag
      661 tcggccatgt gccgcagccg gggccattgc gccgtcatgg gcaccgcctc taccatggcc
      721 tgcatggtcg agagcctggg tctttccttg ccgggcaatg catccattcc cgcagcggat
      781 gcacggagaa aagtattggc ccagcgcagc ggcctccgga tcgtggaaat ggtgaaggaa
      841 aacctgcaac tgtccgatgt attgaccaaa aaagcgtttg aaaatgccat ccgggtcaat
      901 gcagccatcg gaggctccac caattttgta ctccacatga ttgccattgc gggccggatc
      961 ggagtggagc tcaagctgga agactttgac cgttgttcca aaggaattcc tttgctggct
     1021 aacctgcagc cctcaggaga attttttgtg gaagacctgt attatgcggg cggcctgcca
     1081 gccgtaatga aagaaatgga aagttttttg cacggcgatg cactgacggt aaacggcaag
     1141 acggtgcatg agaatatatc cagtgccaaa tgttacaacc ctgcggtgat cggcaccctg
     1201 gacaaaccgt ttaaaacgga aaccggcctg gctatcctga gagggaatct ttgcgaaggc
     1261 ggttgtgtga taaaacccag cgccgccagt tcccacctga tgaaacaccg gggccgggcg
     1321 gtggtctttg aggatattga agattataaa aaaagaattg acctgccgga cctggatgtg
     1381 gatgaaacct gcattttagt gcttaaaaat gtgggcccta aaggatatcc cgggatgccc
     1441 gaggtgggca atatggccct gcctaaaaaa attctccaaa agggtattca cgatatggtt
     1501 cgcatctccg acggccgcat gagcgggacc ggatttggca ctgtcgtgct ccacgcttcc
     1561 ccggaagccg cggccggcgg caattttgca gtggtgaaaa cgggggacta catctctctg
     1621 gatgtggacg ctcgcagcct catgctggaa gtttcggagg aagaattggc gctccgcaaa
     1681 gcatcctgga ttgcacccaa acctcttgcc gacagggggt atgtttcatt atatatccgt
     1741 cacgtgaatc aggcccacga gggggctgac ctggacttcc tggttggaaa ttcttcgagc
     1801 gtggtgacaa gggatagtca ttgaagaatc aatagtcaag agtcaagagt caataatcaa
     1861 gagtcaatag tcagtagtca gtagtcagtg ttcagcaatc agcaaatagt aggctgactg
     1921 ctgattactg attgctgact actgtgcttg ttgcactaca gccttttcag ctttagtttt
     1981 taggataaag gtgccaaatg gggccctaca aatattgatt gtacaggtag agggtaatcc
     2041 tatatggagg atggtatg
//
LOCUS       DAOQXT010000234         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_66215_length_1995_cov_6.718557, whole genome shotgun sequence.
ACCESSION   DAOQXT010000234 DAOQXT010000000
VERSION     DAOQXT010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66215_length_1995_cov_6.718557"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1778)
                     /locus_tag="PKM27_19695"
     CDS             complement(72..1778)
                     /locus_tag="PKM27_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNR09551.1"
                     /translation="MQINTTPSGQVYDAIVIGSGISGGWAAKELCEKGLKTLVLERGP
                     MVRHGEYPTANWHPWEVPLRGNMPKDDVEANPVINRCYAFNETTEHFFVRDRQHPYVQ
                     EKPFDWIRGYQVGGKSLLWARQTQRWSNYEFESPLRDGYAVDWPIRYKDLEPWYGHVE
                     RFTGICGDRDGLDNLPDSEVMPGFALNCVEKHIQEAIHKKYNLSRRVIIGRCAHLTHP
                     QDIHLQQGRGQCQMRTQCERGCPYGGYFSSVSSTIPWAEKTGNLTILPDSVVHSIVYD
                     EDLGKASGVRVVDAVSRDLREYRAKIIFVNAATLNTNLILLNSKSNRFPNGLGNDHGL
                     LGHFITFHNYRGNIAAAYDGFEDQYYYGRRPTQAFIPSFRNVYLRESGFLGGYMAYYV
                     ASRAGWGRDHRQDGIGADLKNALSDPGPWRISMGMQGETVPVYENHVRLSDLTDPWGI
                     PQLVTSIGYTENDDKMVQDFLEAGRDMLESAGCKILGAWDNHQAPGLDIHEMGGVRMG
                     RDPKTSMLNDWNQIHLCKNVFVTDGACMTSTGNQNPSLTFMAMTARAANRAVEELKRG
                     NI"
BASE COUNT          445 a          542 c          506 g          502 t
ORIGIN      
        1 gtaatatgtt ttaaaatacc atattacaag tctaaaggaa gcttagcttc ggcctggtat
       61 aaatccaaag atcagatatt cccccttttc aactcctcaa ccgcccggtt tgcagctctt
      121 gccgtcatcg ccatgaaggt gagggaagga ttctggtttc ccgtggaagt catgcaggcc
      181 ccatcggtca caaatacatt tttgcagagg tggatttggt tccagtcgtt gagcatagag
      241 gttttgggat cacggcccat gcgcactccg cccatctcat ggatatccag ccccggtgcc
      301 tggtgattgt cccaggcccc gagtattttg caaccggcgg attccagcat atcccggccc
      361 gcttccagga agtcctggac cattttatcg tcattctccg tatacccgat ggaagtgacc
      421 agttgcggga taccccaagg gtcggttaga tcgctgaggc ggacatggtt ttcatataca
      481 ggcactgttt cgccctgcat gcccatggag atccgccagg gtcccgggtc ggaaagggcg
      541 tttttcaaat ctgctccgat accgtcctgg cggtgatcgc ggccccaccc tgcccgggag
      601 gccacataat aagccatata accacccaga aagccgcttt ccctgaggta gacgttccgg
      661 aaagaaggaa tgaaggcctg ggtcggacgc ctgccgtaat aatactggtc ttcaaatcca
      721 tcgtaagcgg cagcgatatt gccccggtaa ttatgaaagg tgataaaatg gcccaataaa
      781 ccatggtcgt tgcccagccc gtttggaaac cggttcgatt ttgaattcaa caggatcagg
      841 ttggtattga gggtggcggc attgacaaaa ataattttag cccggtattc tctcaggtcc
      901 cggcttacgg catcgaccac ccggaccccg cttgcctttc ccaggtcttc atcatagacg
      961 atggaatgca ccaccgagtc cggcaggatg gtcaggttac ccgttttttc ggcccagggt
     1021 atggtggaag ataccgaact gaagtatcct ccatagggac aaccgcgctc gcactgggtg
     1081 cgcatctggc actggcccct gccctgttgg agatggatgt cctggggatg ggtgagatgg
     1141 gcgcagcggc ctatgatcac cctccgggaa agattgtatt tcttgtgaat ggcttcctgg
     1201 atatgtttct ccacacagtt gagggcaaaa cccggcatga cttcgctgtc cggcaggttg
     1261 tccagcccat cccggtctcc gcaaatgccg gtaaacctct ccacatgacc gtaccagggc
     1321 tcgagatctt tataccggat cggccagtct accgcgtaac cgtcccggag gggggattca
     1381 aactcataat tgctccaacg ctgggtctgt cttgcccata acagggattt cccgccgacc
     1441 tggtatccgc ggatccagtc aaaaggcttt tcctggacat agggatgctg gcggtcacgt
     1501 acaaaaaaat gctcggtggt ttcattaaaa gcataacacc ggttgatcac gggattggct
     1561 tcgacatcat ccttcggcat gtttccgcga agcggcactt cccagggatg ccagttggcc
     1621 gttggatatt ctccatggcg gaccatgggc cctctttcca aaacgagggt cttcaaaccc
     1681 ttttcacaaa gttctttagc cgcccacccc ccgctgatcc ctgaaccgat gacgatggca
     1741 tcgtaaacct ggccggaagg agtggtattg atctgcacga cgcaatttat gtggaattgc
     1801 ggaattgtgg aatcgttgaa ttgcggaatt gtgaaattga ggaattgttt ccggagtaca
     1861 tttttattcc tctgatcaat ttactatacc ccgatttctc tgacctctta cctctaacct
     1921 atgactaccg accactgacc actgactact cctatgtatc tttttcggtc tcttcctggg
     1981 aaacgtgtcc catat
//
LOCUS       DAOQXT010000235         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_70941_length_1914_cov_5.082302, whole genome shotgun sequence.
ACCESSION   DAOQXT010000235 DAOQXT010000000
VERSION     DAOQXT010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70941_length_1914_cov_5.082302"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="PKM27_19700"
     CDS             complement(<1..1269)
                     /locus_tag="PKM27_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09552.1"
                     /translation="MSKKNNTPEKKPLKKEREFNYPWTLILFGMTFILYLATSRFDFT
                     TEDSRLISKNDLVRQGWKGIPRLFTDLPVDTEGRPTGDYTPASQSLFALEGGLFGMDP
                     RPMHFLQVIYYCLLVVLAYRFIAMILTAWPKPVSLAIAFLFAFHPLHSEGVASLHHRD
                     EILAALGILSALLICLGTTKGTVFRSVMVFIVSCLAFFSKGQAWVLLLLAPLTVWFFQ
                     RNRLKNQALNFTAIGLAALLSFIIKYGVAGVTAPILSLEDNALLIFSGMERTWAAFAM
                     AGHVVRLFLFPVGLQADYSYNQLNLSAWSDPWAAIGFLLLGAGIYFMAKGLKARSIYS
                     YALFFCLAFYLPVSNLFLMNARTFTEAQLLLPSLELLILAVPVFLRLAEKYQLGRNGR
                     RAVLLAVLGLYALQVFLRIPDWRNNKTIYTV"
     gene            1380..>1914
                     /locus_tag="PKM27_19705"
     CDS             1380..>1914
                     /locus_tag="PKM27_19705"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNR09553.1"
                     /translation="MKLLSVVLLVFSMFLARGQGHSIIPAPASWTEGTGTFNLSAKTG
                     IVIIGYPVEAQKVADFFRAMPLVKNLNMSGKVTGAGNIRLELLDSADSGLGKEGYDLD
                     ISGGGIQLRANSAAGLFYGVQTIHQLIPVTGTITPNSTLRIPACKIRDTPRLGWRGLM
                     LDVSRHFFTVDEVKQYID"
BASE COUNT          524 a          489 c          508 g          393 t
ORIGIN      
        1 aacggtatag atggttttat tattacgcca gtccgggatc ctgagaaaga cctgaagcgc
       61 ataaagaccc aggacggcta gcaagactgc ccggcggcca ttgcgcccca gctggtactt
      121 ttcggcaagc cggaggaaaa cgggaaccgc caggataagc aattcaagcg acggcagcag
      181 caactgcgct tctgtgaacg tcctggcatt cattaaaaaa agattggaaa cgggcaggta
      241 aaacgcgagg caaaaaaaga gggcatagct gtaaatcgat ctggccttca acccctttgc
      301 cataaaatag attcctgcgc ccaaaagaag aaatccaatg gctgcccagg gatcggacca
      361 agccgaaaga ttcaactggt tgtaagaata atccgcctgg aggcccaccg ggaataaaaa
      421 caagcggacc acatggcccg ccatcgcaaa agccgcccag gttctttcca tgcccgaaaa
      481 gatcaacagg gcattgtctt ccaggctgag gattggggcg gtcactccgg ctaccccgta
      541 tttaataata aaagagagca aggccgcaag tcctattgcg gtaaaattca aagcctggtt
      601 tttgagccgg tttcgttgga aaaaccagac agtaagcggg gccagcaaca gcagcaccca
      661 cgcctggccc ttggaaaaga aggcaagaca ggaaacgata aataccataa cggatcggaa
      721 gaccgtgccc ttggtggtgc ccaggcaaat gaggagggct gaaaggatgc ccaaggccgc
      781 aaggatctca tcccggtggt gtaggctggc caccccttcg ctgtgcaggg gatgaaaggc
      841 aaacaaaaat gcaatggcca gcgaaacagg tttaggccat gccgtcagga tcatggctat
      901 aaaccggtag gccaggacga ccagcaagca ataataaatc acttgcaaaa agtgcatggg
      961 cctcggatcc atgccaaata agccgccttc caaggcaaac aggctttggc tggcaggagt
     1021 atagtccccg gtaggccggc cttccgtgtc caccggcagg tccgtaaaca aacggggtat
     1081 ccctttccat ccttgccgga ccaggtcatt ttttgaaatc aggcgggaat cttccgtagt
     1141 aaaatcaaac cggctggtcg ccaggtaaag gataaaggtc ataccgaaca ggatcagggt
     1201 ccaggggtaa ttgaattccc gctctttttt caacggtttt ttttccggtg tattattttt
     1261 ttttgacata ccaatgaata cgggcggcaa gatagggatg gagaatgaat cggatcggga
     1321 ttcataccag ccgcctcccc tattttccct aattttgtgc tctcaaaaca ttaatccaaa
     1381 tgaagctatt gtctgtcgtt ttattggtct tttccatgtt cttggcccgg ggtcaggggc
     1441 attccataat tcccgcaccg gcatcctgga ctgaaggaac cggcactttc aacctcagtg
     1501 ccaaaaccgg aatagtgatc ataggttatc cggtggaagc gcaaaaggtc gccgattttt
     1561 tccgggccat gcctttggtc aagaacctga acatgagcgg caaggtgacg ggagccggaa
     1621 acatccggtt ggaactcctg gacagcgcgg attccggact gggcaaggaa ggatatgacc
     1681 tcgacatcag tgggggcggg atccaactgc gcgccaacag cgccgccgga cttttttacg
     1741 gagttcagac catacaccag ttaatcccgg ttaccggcac catcactcca aactccacgc
     1801 tgagaatccc tgcctgcaaa atccgggaca cgccccgcct gggatggagg ggcctgatgc
     1861 tcgatgtcag ccgccatttt tttacggtgg atgaagtaaa acaatatatc gatg
//
LOCUS       DAOQXT010000236         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_71361_length_1907_cov_6.291037, whole genome shotgun sequence.
ACCESSION   DAOQXT010000236 DAOQXT010000000
VERSION     DAOQXT010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71361_length_1907_cov_6.291037"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKM27_19710"
     CDS             <1..634
                     /locus_tag="PKM27_19710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNR09554.1"
                     /translation="KLAAEAWEQGRFRDEVVKVEVDAGKGKTSVVDKDEEYARVNFEK
                     IPLLKPAFNKEGTVTAANASTLNDGAASILLVSGEMLKEFGLKPLARIVSFADAEQEP
                     KWFTTTPTVAAPLALRKAGLELKDMDYFEVNEAFAVVALAFIQLLHLDPDRVNIHGGA
                     VALGHPLGASGARILVTLAHILRQKKARYGLAAICNGGGGASAVILENMQ"
     gene            902..1456
                     /gene="msrA"
                     /locus_tag="PKM27_19715"
     CDS             902..1456
                     /gene="msrA"
                     /locus_tag="PKM27_19715"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNR09555.1"
                     /translation="MDQNTKYEIATFGGGCFWCTEAIFLELEGVVKVESGYSGGHVDN
                     PTYKEVCTGNTGHAEVIQITFDPAKVSFEEVLLVHFTTHDPTTLNRQGNDVGTQYRSV
                     IFYHDEAQKAAAEKAKAEVAPALWANKPITTEIVPFEKFYKAEDYHQNYLEENPNQPY
                     CIYVVNPKVEKFRKAFKDKLKKHE"
     gene            1449..1817
                     /locus_tag="PKM27_19720"
     CDS             1449..1817
                     /locus_tag="PKM27_19720"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HNR09556.1"
                     /translation="MNEGFNPLTPEEEYVIVHKGTERPFSGEYDQFSGQGTYICRRCN
                     APLYRSEDKFHSGCGWPSFDDEIPGAVLKKPDPDGRRTEILCANCQGHLGHVFFGEGF
                     TPRNTRHCVNSLSMRFVGKK"
BASE COUNT          496 a          489 c          500 g          422 t
ORIGIN      
        1 taaactcgca gcggaagcct gggaacaggg acggttcagg gatgaagtgg tgaaggtgga
       61 agtggatgcc ggaaaaggca aaacctccgt ggtggacaag gacgaggaat atgcccgggt
      121 caattttgaa aagatacctc tcctcaagcc ggccttcaac aaggaaggga cggtcaccgc
      181 ggcaaatgca tccacgctca acgatggggc ggcatccatt ctgctggttt ccggggaaat
      241 gctgaaagag tttggactga agcccctggc caggattgtt tcttttgccg atgcggaaca
      301 ggaacctaaa tggtttacca cgaccccgac tgtggcggct cccctggccc tgcgcaaagc
      361 cggcctggaa ctgaaggaca tggactattt tgaagtgaac gaagccttcg cggtcgtggc
      421 ccttgccttt attcaactgt tgcacctgga tccggaccgg gtgaatattc acggcggagc
      481 cgtggccctg ggtcatcccc tgggcgcctc aggtgcccgg atcctggtga ccctggctca
      541 tattctccgg cagaagaaag ccaggtatgg gcttgcggcc atctgcaacg gaggcggggg
      601 agccagcgcg gtgattctgg agaatatgca ataaggggat aaaacagggg ctgtctcgaa
      661 gttaccagaa aacaggaggt gtctttgaag acaccccctg ttttaacttc gagacaaccc
      721 ctgttttatc tccgacgcct cctggtttat cccgaacttt tgtattttga aactgtttat
      781 taaaaataac aagccccttc ctatgaaaac attttccctc ttattcctgg tttttgggat
      841 ccaggcttgc ccaaatccat ccgggaattc aatcaagact gaaccaacta cttcaattcc
      901 tatggaccag aatacgaaat acgaaatagc cacttttggc ggcggatgtt tttggtgcac
      961 cgaagccata ttcctggaac tggaaggtgt ggtcaaggtg gaatccgggt attccggagg
     1021 ccatgtcgat aatcccacct acaaggaagt ctgcacgggg aataccggcc atgccgaagt
     1081 catccagatc acttttgatc cggccaaagt cagtttcgag gaggtgctgc tggttcattt
     1141 caccacacac gaccctacga ccctcaaccg gcagggcaac gatgtaggca cccaataccg
     1201 gagtgtgatt ttctaccacg atgaagccca gaaagcggcg gcggaaaagg ccaaagccga
     1261 agtagccccc gccttgtggg ccaacaagcc catcaccacc gagatcgtcc cctttgaaaa
     1321 gttctacaaa gccgaggatt accatcagaa ctacctcgaa gaaaacccaa accagcccta
     1381 ttgcatctat gtggtcaatc cgaaagtgga aaaattcagg aaagccttta aggataaatt
     1441 gaaaaaacat gaatgaggga ttcaaccccc tgaccccgga ggaagaatac gtcatcgtgc
     1501 ataaaggcac ggaaaggcct ttttccggtg aatacgacca attcagcggg caagggacct
     1561 atatctgcag gaggtgcaac gcccccctct accggtctga ggataaattc cacagcggtt
     1621 gcggatggcc cagttttgac gatgaaatcc ccggcgctgt attaaaaaaa ccggacccgg
     1681 atggacgccg tacggaaatc ctctgcgcca attgccaggg ccatctcggt catgttttct
     1741 ttggggaagg attcacaccc aggaatacca ggcattgtgt gaattcactg agtatgaggt
     1801 ttgttggtaa gaagtaaatc agttagcaag ttagcaagtt agcaagtttg ttagttgcat
     1861 gctcgcatct cgcatctcgc atctcgcatc tcgcatctcg tatctcg
//
LOCUS       DAOQXT010000237         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_72560_length_1888_cov_6.511184, whole genome shotgun sequence.
ACCESSION   DAOQXT010000237 DAOQXT010000000
VERSION     DAOQXT010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72560_length_1888_cov_6.511184"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKM27_19725"
     CDS             <1..695
                     /locus_tag="PKM27_19725"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNR09557.1"
                     /translation="FMLSLLDSTGNADPERQMTIRALRNWNFHFGPDAPEATFFNTWY
                     RKMYRLTFDELYGSKDSSQLQYPKMYFTSMLLKDEDHSIFDLKSTADLSETARDIVNL
                     AFNQAWQEVDRDPDGKILPWSQASKSKVQHLANLPGFSRENIKIGGIGDALNAMANGS
                     GPSWRMIVELGEKPRARVIFPGGQNGNPGSRYYDNMIDDWASGKYQEAHFVKQAEEVK
                     ALYTQHFSAGHE"
     gene            688..1041
                     /locus_tag="PKM27_19730"
     CDS             688..1041
                     /locus_tag="PKM27_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09558.1"
                     /translation="MNNKILYGLLVFLVLGYLMQFFLPWYSLAFAGIAAGYMSKGKPA
                     QSLALFFLAGVIFWGLAAYLKDSASSSILSARIGILFGDQPPGALLGLTALIGGITGG
                     LGGWCGALFGKYIRQ"
     gene            1130..1561
                     /locus_tag="PKM27_19735"
     CDS             1130..1561
                     /locus_tag="PKM27_19735"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HNR09559.1"
                     /translation="MDKDTVTYLKAEKRVKEMKSFYRFLFTWIVLSSFFMALNFFSSP
                     GDFWAIYPTLAIGLAVAIRYIRVFGWPGFGKEWEERKFYEEVERIKEREERIKYMLDH
                     GRSSRPLPKAKDPNQAAPDIDKLELKEIKEIRKQWRDSEFV"
BASE COUNT          482 a          463 c          462 g          481 t
ORIGIN      
        1 ccttcatgtt atccttgctg gacagcacgg gcaatgcgga ccctgaaagg cagatgacga
       61 tccgtgccct gagaaactgg aattttcatt ttggaccgga tgctccggaa gccacctttt
      121 ttaatacctg gtacagaaag atgtaccggc tgacttttga tgaattatat ggcagcaaag
      181 actcttccca acttcagtat cccaagatgt atttcaccag tatgctcctg aaggacgagg
      241 accactctat ttttgatttg aagtccactg cagacctgtc cgaaaccgcc agggacatcg
      301 taaacctggc tttcaaccaa gcctggcagg aggtggaccg ggatccggac ggaaaaattc
      361 tgccctggag ccaggcgtcc aaatccaaag ttcagcacct ggccaacctt cccggttttt
      421 caagggaaaa tataaagatc ggagggattg gcgatgccct caatgcgatg gccaacggtt
      481 ccggcccttc ctggagaatg attgtggagt tgggggaaaa accccgggcc cgggtgatct
      541 tccccggggg ccagaatggg aaccccggct cccggtatta tgataatatg atcgatgact
      601 gggcctccgg aaaataccag gaagcccatt ttgtcaaaca agccgaagaa gtcaaggcat
      661 tatacactca acatttttct gccggtcatg aataataaaa tcctttacgg tctgctggtt
      721 tttttggtcc tcggatatct gatgcagttc ttcctgccct ggtacagcct ggcctttgcg
      781 ggtattgctg ccggatatat gtccaaagga aaaccggccc agagcctggc gctctttttc
      841 cttgccgggg taatcttttg gggcctggcc gcctacctaa aagacagcgc atcctcttcg
      901 attttgtcag ccaggatcgg catcttattt ggggaccagc ctccgggagc cttgctgggc
      961 ctgacggccc tgattggagg catcacgggt ggcttgggag gctggtgcgg tgcattattc
     1021 ggaaaataca tacgccagta agtcactttg gtcgatcatt tcgggttcct tttcaggaaa
     1081 tcttgcatgg aaccggcatt ttcctcaatt ttgataaaac tccggaccaa tggataaaga
     1141 caccgtcacc tatctcaagg cagaaaagag ggtgaaagaa atgaaatctt tctatcggtt
     1201 tcttttcacc tggattgtct tatccagttt ttttatggcg ctgaattttt tcagcagccc
     1261 gggtgatttc tgggcgattt atccaacgct ggccatcggc ctggcggtcg cgatccggta
     1321 tatccgcgtt ttcggatggc ccggcttcgg aaaagaatgg gaggagcgta aattttatga
     1381 ggaagtggag cgcattaagg aaagggaaga aagaataaaa tacatgctgg accacggccg
     1441 cagctcccgt cccctcccga aagccaaaga ccccaatcaa gccgccccgg atatcgacaa
     1501 actggaactg aaagaaatca aagaaatccg gaagcagtgg cgggactccg agtttgtctg
     1561 agggcggaga gcgtgggatg aggcctgaag tcctgccacc tcctgcgtgc tacctctaac
     1621 ctctaacctc taacctctaa cctcttaccc ctaacctcca tttcacaaat attaaaaaac
     1681 tctatattcc ttgtacaaat gtgagtttaa tctatatttg cacaggccat acaaggttat
     1741 tctttttcaa gacgacaatt ataattccaa tgacgtgtat ggcctttttt tatttcagca
     1801 atcagcaatc agcattcagc attcagcaat cagtagggaa gttcacgagt agcctaagga
     1861 gttgagactt aggaacatat taaaattt
//
LOCUS       DAOQXT010000238         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_72723_length_1885_cov_5.731694, whole genome shotgun sequence.
ACCESSION   DAOQXT010000238 DAOQXT010000000
VERSION     DAOQXT010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72723_length_1885_cov_5.731694"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..850)
                     /locus_tag="PKM27_19740"
     CDS             complement(77..850)
                     /locus_tag="PKM27_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR09560.1"
                     /translation="MKIALFSDIHANLPALEACLKSIGEQKPDAVYCLGDLVGYNIWP
                     NEVIQEIRKRGIPTIAGNYDQGIGLQSDDCGCAYKTESEKEMGKISISYTNSVVKPDE
                     RAYLRTLPAHIRVEFQLNRDKLNLLLVHGSPRKINEYLFEDRDEKSLLRIMEQADADI
                     LCFGHTHKPYHRILPEGQAEGSHYRHAVNLGSVGKPKDGNPKGGYVMLTILENSSITN
                     KNSIQAEFIRFDYDIEKAARAVEESPLPNEYAEMLRRGF"
     gene            complement(847..>1885)
                     /gene="arsB"
                     /locus_tag="PKM27_19745"
     CDS             complement(847..>1885)
                     /gene="arsB"
                     /locus_tag="PKM27_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNR09561.1"
                     /translation="DRHLTLWIFMAMAAGVSIGYFFPEAAGFINSYSSGTTNIPLAIG
                     LILMMYPPFTKVRYDKLKEVFRNTKVLSVSLLLNWIIGPIVMFLLALVFLGNHPEYMA
                     GLILIGLARCIAMVIVWNELAGGSREYAAGLVALNSIFQVLMFSVYAYVFITVLPPVL
                     GFSGLQVDITIGQIAESVAIYLGIPFLAGFLTSIFLSKWKGEAWYRSKFIPFVSPVTL
                     IALLFTIVIMFSLKGDLIIQIPLDVLRIAAPLLLYFVIMFLVSFVAGKKMGADYSTSA
                     SIAFTAAGNNFELAIAVAIGVFGINSGQAFAGVIGPLVEVPALIALVNVAYWIQKRFY
                     PSVQHSKYLSK"
BASE COUNT          513 a          475 c          434 g          463 t
ORIGIN      
        1 tattattcac aacaatctgc aggcgggcct gcccgacttg ctatccttaa caatcttcag
       61 gcgggccatg gcttaatcaa aatccccttc tcagcatctc cgcatattca ttgggcaaag
      121 ggctttcttc caccgccctg gccgcctttt caatatcata atcaaagcgg ataaattcgg
      181 cttggatcga atttttattt gtaatgctgc tgttttccag tatagtcagc atgacataac
      241 ctcccttggg attgccgtct ttgggttttc ccacagagcc taagttgacg gcgtgccggt
      301 aatgactccc ttcagcctgg ccttcgggca gtatccggtg gtagggtttg tgggtatgcc
      361 caaaacaaag gatgtcggca tctgcctgtt ccatgatccg caggaggctt ttttcatccc
      421 tgtcctcaaa caggtattca ttaatcttcc tcggacttcc atgaacaagc aaaagattca
      481 atttgtcccg attgagctgg aattcaaccc tgatatgggc cggcagggtc cggagatagg
      541 ctctttcatc cggttttacc actgaattgg tgtaggaaat ggagattttg cccatctctt
      601 tttctgattc cgttttataa gcgcagccgc agtcgtcgct ttgcagccct ataccctgat
      661 cgtaatttcc ggcaatggtt ggtatcccgc gctttctgat ttcctggatg acctcattgg
      721 gccagatatt gtaaccaacc agatcaccca ggcaatagac cgcatccggt ttttgttccc
      781 caatactctt taaacaggct tccagggcag gcaggttggc atgaatgtcg gaaaataagg
      841 cgattttcat ttggacaggt attttgaatg ttgaacagac ggatagaaac gtttttggat
      901 ccaatatgcc acattgacca aagcgatcag ggcaggcact tccaccaaag ggccgataac
      961 gcccgcaaaa gcctgtccgc tgtttatgcc gaacacccca atggcgaccg caatggccag
     1021 ttcaaaatta ttccctgcag cggtaaaggc gatggaggca ctggtggaat aatcggctcc
     1081 catttttttt ccggctacaa aactgaccag gaacatgatg acaaaataca gaaggagcgg
     1141 ggccgcaata cgcagcacat ccagggggat ctgaatgatc aggtctcctt tgagactgaa
     1201 catgatcacg atggtaaaca gcaaagcgat cagcgtgacc ggggatacga agggaatgaa
     1261 ttttgaccga taccaggcct cccctttcca ttttgacaag aatatactcg taagaaaccc
     1321 cgcgaggaaa ggaatgccca ggtaaatggc caccgattcc gcaatctgtc caatggtgat
     1381 gtcgacctgc aaaccggaga atcccaaaac cggtggaagc acggtaatga atacataagc
     1441 atatacactg aacatcaata cctgaaagat gctgttgagc gccaccaaac ccgcggcata
     1501 ttccctgctc ccacccgcca gttcattcca aacgatgacc atggcaatac accgggctag
     1561 gcctatgagg atgagcccgg ccatatattc aggatggttt cccaggaaaa cgagagccag
     1621 taaaaacatg acgattggcc caatgatcca attgagcaat aaagaaacgg ataaaacctt
     1681 cgtgtttcgg aaaacctcct tcagtttatc ataccggacc tttgtaaaag gaggatacat
     1741 cattaatatc aacccaatgg ccaatggaat attggtggtc ccggatgaat aggaattgat
     1801 aaatccggca gcctccggga aaaaataacc aatggatacc cctgcagcca tggccataaa
     1861 tatccacagt gtgagatgtc ggtcc
//
LOCUS       DAOQXT010000239         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_73920_length_1867_cov_6.904525, whole genome shotgun sequence.
ACCESSION   DAOQXT010000239 DAOQXT010000000
VERSION     DAOQXT010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73920_length_1867_cov_6.904525"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="PKM27_19750"
     CDS             <1..1774
                     /locus_tag="PKM27_19750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNR09562.1"
                     /translation="TSFGWYDFYTYLKLAPGTDPGRLQEKFPAFCDRYINSLEWAQKN
                     NYRNEIHLIPLTDIHLYSNVNQEAEINGNGQAVSFLFLIAFLIIGIAWINYINLATAR
                     SMERAREVGVRKVAGAQKANLVTQFLMESLMLNAVALLFALGFTALLTPWFNRLTGFD
                     SSQSGIFNTSYLGYFLLVFTMGALLSGLYPAFVLSGFRPVTVLKGLFKNSPGGLMLRK
                     GLIVTQFVTSVALIAGTIIVYQQVKYMRSQSLGANIDQTLVLQGAESPADSGYQEIFQ
                     PFKNQLLQQTGIQSITASSSVMGQEIYWTSGIRALRPGSQSVTHYILGVDQDFVPSFG
                     LKLAAGRNFSKEFPSDSRAVLLNEKAVELLGFESPEKALNESVIRGDTFKIIGVLGNY
                     HHLGLQKEINPMLVLLRPNARNYYSVKINTGDLGSAIETIGKTWNSFFPSDPFSYYFL
                     DASFDQQYRADRRFGEVFGIFSLLAILIACFGLLGLSAYNILQRTKEVGIRKVLGAPV
                     ANILYILSRDFMILVGFAFLLAVPLCWWLMETWLKDFAYRIDISWWVFAVAGFITLII
                     AFATVAGQALHAAHAKPVKSLRME"
BASE COUNT          433 a          464 c          471 g          499 t
ORIGIN      
        1 gaccagcttc ggctggtatg atttttatac ttacctgaaa ctcgccccgg gtacggaccc
       61 cggacggttg caggaaaaat ttcccgcttt ttgcgaccgc tacatcaaca gtctggaatg
      121 ggcgcaaaaa aacaattacc ggaatgagat ccacctgatc ccgcttaccg atatccacct
      181 atattccaat gtgaaccagg aagccgaaat aaacggaaac ggacaagccg tatctttcct
      241 ctttctgatt gctttcctca taatcggcat cgcctggatc aattacatca acctggctac
      301 ggcccgatcc atggaaagag cccgggaagt gggcgtccgc aaagtggccg gagcccaaaa
      361 ggccaacctg gtcacgcagt ttttaatgga aagtttaatg ttgaacgcgg tggctctttt
      421 atttgcccta ggatttaccg ccttgcttac tccctggttc aaccggctta cgggttttga
      481 ttccagtcaa agcgggatat ttaatacgtc ctacctgggg tattttctgc tggtatttac
      541 gatgggagcc ctgttgtccg gtttgtaccc ggcctttgtt ttgtccggct tccgcccggt
      601 caccgtgctg aaaggcttgt tcaaaaacag tcccggggga ctgatgcttc ggaaaggcct
      661 gatcgtcacc cagtttgtga cttccgtggc tttgatcgcg ggtacgatca tcgtctatca
      721 acaggtgaaa tatatgagaa gccagagcct gggggccaat attgatcaaa ccctggtctt
      781 gcaaggagca gaatcgccgg cggattccgg ctaccaggaa attttccagc cattcaaaaa
      841 ccaattgttg cagcagaccg ggattcaaag catcaccgct tcatccagcg tcatgggaca
      901 ggaaatatat tggaccagcg gcatcagggc cctccggccg ggaagccagt cggtgaccca
      961 ttatattctt ggtgtggacc aggattttgt accctccttt ggcctgaaac tggctgccgg
     1021 aagaaacttt tccaaagaat ttccatcgga cagccgtgcg gtgttgttaa atgaaaaggc
     1081 tgttgaattg cttggatttg aatctcccga aaaggcgctg aatgaatcgg tgatccgggg
     1141 ggataccttt aaaatcatcg gagtgcttgg caactatcat cacctgggtc tgcaaaagga
     1201 aatcaatccc atgctggttt tgctgaggcc caatgcccgg aactattact ccgtcaagat
     1261 caataccggt gacctgggtt cagcgataga aaccatcggg aagacctgga attctttttt
     1321 cccaagtgat ccgttcagtt attatttcct ggatgcttca tttgatcagc agtaccgggc
     1381 cgaccgccgc tttggggaag tctttggcat cttcagcctg ctggccattc tgatcgcctg
     1441 ttttggactg ttggggcttt cggcttacaa tatcctgcag cggaccaagg aagtcggcat
     1501 caggaaggtc ctgggcgccc cggtggccaa tatcctgtat atcctctccc gggattttat
     1561 gatcctggtg ggatttgcat ttctattggc cgttcccctt tgctggtggt tgatggaaac
     1621 ctggctgaag gattttgcct accgcatcga tatcagctgg tgggtattcg ccgtggcggg
     1681 ttttattacc ctcatcattg cttttgccac cgtcgctggg caggccctcc atgcagccca
     1741 tgccaaaccg gtgaaaagcc tcaggatgga atagtagtca gtagtcaata gtcagtagtc
     1801 aatagtcagt agtcagtagt gcttgttgca caactctgaa aataatcata ttaaaatatt
     1861 tgttata
//
LOCUS       DAOQXT010000240         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_77400_length_1816_cov_6.587166, whole genome shotgun sequence.
ACCESSION   DAOQXT010000240 DAOQXT010000000
VERSION     DAOQXT010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77400_length_1816_cov_6.587166"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1423)
                     /locus_tag="PKM27_19755"
     CDS             complement(212..1423)
                     /locus_tag="PKM27_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR09563.1"
                     /translation="MLLKTAIIGGGAAGYFAAIRYKSLHPGHKTVIFEKTGKVLGKVK
                     ISGGGRCNVTHACFVIKDLIGYYPRGARELIGPFHRFQPADTMKWFEDRGVALKTEPD
                     GRVFPVSDKSQTIIDCLTGEAERLGIELHTSQDLESIGLGESGGFQLQFAGKPGHVFA
                     DRLIVTTGSSEKVWKILETMGHRILPPVPSLFTFNIRDERLLDFQGLTWPDVEVRDLD
                     FGHRATGPLLITHWGLSGPAVLKLSAIGALDFHKAGYRFEIAVNFMFPDKEDVVAAVF
                     QKLKQNHPNKLVSQSPPGPFTARLWQSWSSLWGFGNKTWQEVSMKEIGFMARQITEAK
                     FKVDGKSTFKEEFVTCGGVDLKEVDLKTMESKKIPGLYFAGEVLNIDALTGGFNFQAA
                     WTTGWLAGSHG"
     gene            complement(1428..>1816)
                     /locus_tag="PKM27_19760"
     CDS             complement(1428..>1816)
                     /locus_tag="PKM27_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR09564.1"
                     /translation="VNIFGVTNWLRTLKSIPPYWESFRKALYDEMGDPYSADSVRLFN
                     ISPLFHAARVTKPLMVLQGANDPRVLQVESDEIVEAVRKNKVPVEYVLFPDEGHGFLK
                     KENEIKGYGQVGVFLDQYLKKEQVKN"
BASE COUNT          434 a          504 c          406 g          472 t
ORIGIN      
        1 aattttttgt gtccttcgtg cctttgcggg aaacctatag tgtcctggta ggaaatctct
       61 taaagcaatt atcccaagca tcgaagatgc tccacgtttt taacactcat tccaggacat
      121 cctcggcttc gctgtgccgt tgtgggaaat tctttgtgac tttgcgtcgt cgtgggaaaa
      181 ttttgtgtcc ttcgtgcctt tgcgggaaaa tctagccatg gctccccgcc aaccaccccg
      241 tggtccaggc cgcctggaaa ttaaagccac cggtaagcgc atcgatgttc agcacttcac
      301 cggcaaaata caaacccggg atttttttac tttccatggt cttcaagtcc acttctttca
      361 agtccacccc cccgcaagtc acaaattctt ccttaaacgt actcttgcca tcgaccttga
      421 atttcgcttc cgtgatctgc ctggccataa atcctatttc tttcatggat acctcctgcc
      481 aggttttatt gccaaaaccc caaaggctgc tccaggactg ccataacctc gcggtaaaag
      541 gacccggagg agactggctg acgagtttat taggatggtt ttgttttaat ttttgaaaaa
      601 cagcggctac cacatcctct ttatctggaa acataaaatt cacggctatc tcaaaacggt
      661 acccggcctt atggaaatca agagcgccaa tagcggagag ttttaacacc gcggggccgc
      721 tcagccccca gtgcgtaatc agcaatggcc ccgtggcccg gtgaccaaaa tccaggtccc
      781 tgacctcaac atcaggccag gtcagtccct gaaaatcgag cagccgttca tcccggatgt
      841 taaaagtaaa gagcgaagga accggaggca ggatccgatg acccattgtt tcgagtattt
      901 tccatacttt ttcgctggat ccggtggtga cgatcagccg gtccgcaaaa acatgccctg
      961 gctttccggc aaattgaagt tgaaagcctc cgctctcacc aagccctatg gattccaggt
     1021 cctgcgatgt gtggagttct attccaagcc gttcggcctc cccggtgagg caatcgataa
     1081 tggtttggga tttgtccgac accgggaaca cccttccatc cggttccgtc tttaaggcaa
     1141 cgccccggtc ttcaaaccac ttcatggtat ctgccggctg gaaccggtga aaagggccga
     1201 tcagttccct ggcgccacgt ggataatacc cgatcagatc tttgattaca aaacaggcat
     1261 gggtgacatt gcacctgcct cccccggata ttttaacttt gcccaggact ttgccggtct
     1321 tttcgaatat caccgtcttg tggccaggat ggagcgactt ataccggatg gcggcaaaat
     1381 aacccgcggc tcctccccct atgatagcgg tcttcagaag caatggatta atttttcact
     1441 tgttcctttt tcagatattg gtcaaggaag acccctacct ggccatatcc tttgatttca
     1501 ttttcctttt tcaaaaaacc atggccctca tcgggaaaga ggacatactc caccggtacc
     1561 ttgttttttc ttaccgcctc tactatctca tcggattcga cctgcaggac cctcggatca
     1621 ttggcgccct gcaagaccat cagaggcttt gtgacccggg cagcatgaaa caggggggaa
     1681 atattaaaca accgtacact gtctgctgaa tagggatccc ccatctcatc atataaagcc
     1741 ttacggaaag actcccaata aggcggtatg gatttgaggg tgcgcagcca attggtcacg
     1801 ccaaaaatat tgaccc
//
LOCUS       DAOQXT010000241         1772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_80542_length_1772_cov_4.605708, whole genome shotgun sequence.
ACCESSION   DAOQXT010000241 DAOQXT010000000
VERSION     DAOQXT010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80542_length_1772_cov_4.605708"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="PKM27_19765"
     CDS             complement(<1..1698)
                     /locus_tag="PKM27_19765"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNR09565.1"
                     /translation="MRGLWLPFFFIQIILGFGQTWQQKADYRIHCRLDDQAHQLQARM
                     DLVYHNRSPHTLGQLKLHTWMNVFKTPKSAYSKQLNALGMDQRLGLKKKDEGAYTSLA
                     ISQEGKPLNYSYSDPAAEILVLKLADPIPPGQAGTITIEYVLKLPSNISRGGHLGQSY
                     QLTQWYPKVALYDTSGWRVMPYLELGEFYNEFGDYEVTVELPANYIVAATGQLENPEE
                     TEFLSRYADSCAAKLPSTGPALLSVPSASHYKSLKYKATNVTDFAWFADKRFLVNRKL
                     FELGDRKVEGWTFYLPGRNPVWKSAISHLASATRYFDSLVGPYPWPLISMVQCERHGT
                     DAMEYPMIALIDAGYEQPGSLEEVIVHETGHQWFQGILATDERKQTWMDEGLVTYYEH
                     RYFRERGAPGLLADLMLGPPTDYDRLPDFDWYLQANRRRDAPSGADPLKFSLRGYVQS
                     VYEKPAKGLLFMEKSLGTQAFDRMIQSWFRQWRFRHPGASDFQSLAAAHGASWFYPLY
                     ISGTTKADAAIEPDTRDGVFLVNHNLDFPIPLEYAGFRNGNRLFTKQLPLLLGRDTLR
                     "
BASE COUNT          428 a          440 c          485 g          419 t
ORIGIN      
        1 cctgagagta tcccggccca ggaggagtgg cagttgtttt gtaaaaagcc tgtttccatt
       61 ccggaatccg gcatattcca atggaatagg aaaatcaagg ttatggttga ccaggaaaac
      121 accatcccgg gtatccggtt cgatggcagc atcggccttc gtggtccccg aaatataaag
      181 aggataaaac caggaagcac catgggctgc ggccagggat tgaaaatctg aggctcccgg
      241 gtgcctgaag cgccattgcc tgaaccagga ctggatcatc cggtcaaagg cctgggtgcc
      301 caaagatttt tccataaaaa ggaggccttt cgccggcttt tcataaacgg attggacata
      361 tccgcgaagg ctgaatttta gagggtcagc tcctgaagga gcatcccgcc ggcgattggc
      421 ctgaaggtac caatcgaaat ccggcaagcg gtcataatcc gttggcgggc caagcatcag
      481 atccgccaac aggcccggag cgccgcgttc ccggaaatag cggtgttcat aataggtgac
      541 caggccttca tccatccagg tctgttttct ttcatcggtg gccaggatgc cctgaaacca
      601 ttggtgtccc gtctcatgca cgatgacttc ttccaggctt cccggctgct cgtaaccggc
      661 atcgatgagt gcaatcatcg ggtactccat ggcatcggtt ccatgcctct cgcattgcac
      721 catgctgatc agcggccagg ggtaggggcc aaccagactg tcgaaatacc gggtagctga
      781 ggccaggtgg gaaatagccg atttccaaac cgggtttcgt ccgggcaggt aaaaggtcca
      841 tccttccact ttgcgatcgc caagctcaaa aagcttacga tttaccagga accgtttatc
      901 ggcaaaccag gcaaaatcgg tcacattagt cgctttatac ttcagggact tgtaatggct
      961 ggcggaggga accgaaagca aagccggtcc ggtcgaaggg agctttgcag cgcaggaatc
     1021 ggcataccgg gatagaaatt cagtttcttc cgggttttcc agctggccgg tagccgccac
     1081 gatatagttt gcaggtaatt cgacggtgac ttcgtaatcc ccgaattcat tgtagaattc
     1141 tccgagttcc agataaggca tcaccctcca gccggaagta tcatacagag cgactttggg
     1201 ataccattgc gtcaattggt aagactgacc caggtggccg cctcttgaaa tattggaagg
     1261 aagctttaaa acatactcta tggtaatggt tccggcctgt cccggtggga tgggatctgc
     1321 cagtttgagg accaggattt ccgctgcagg atcggaataa gagtagttca aaggcttgcc
     1381 ttcctgggaa atagccaggg aagtataggc cccttcgtcc ttttttttaa gcccgagtcg
     1441 ctgatccata ccgagcgcat tcaattgttt tgaataggcg gactttgggg tcttaaaaac
     1501 gttcatccag gtgtgtaatt tcaattgacc cagggtatgg ggtgaccgat tatggtaaac
     1561 caaatccatc cgggcctgca attggtgggc ctggtcgtca agccggcagt ggatgcggta
     1621 atcggctttt tgttgccagg tctggccgaa tcccaggatg atctgaatga aaaaaaacgg
     1681 gagccagagg cctctcatgg gctaaaattc gtcattccgg ttcgccgtca aagcggttga
     1741 ttcaatttcc gtaattttgg accatgctac cg
//
LOCUS       DAOQXT010000242         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_90268_length_1652_cov_5.950532, whole genome shotgun sequence.
ACCESSION   DAOQXT010000242 DAOQXT010000000
VERSION     DAOQXT010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90268_length_1652_cov_5.950532"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..799)
                     /locus_tag="PKM27_19770"
     CDS             complement(113..799)
                     /locus_tag="PKM27_19770"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNR09566.1"
                     /translation="MKRSRAGNSIRNTLLILAAIWTGFFIQNLPFFNTYRWGIIPRYT
                     ESLKGIITAPLMHGDWAHLISNSAPMAVLSMMLFYFYRKIAWVSFTLIWVLCGLAVWL
                     FARGSSVHIGASGVIYGLVAFIFWNGIFRRDLKSIILLLIVTILYSGMMFGILPHQEG
                     ISWESHLFGGIVGIFIAYFMRRFSIKEEDDIELEEPKSLEEGDKEYYFDRDVFEKTLR
                     EKERGLRGEE"
     gene            complement(872..>1652)
                     /locus_tag="PKM27_19775"
     CDS             complement(872..>1652)
                     /locus_tag="PKM27_19775"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNR09567.1"
                     /translation="GGTILQSARSETFRTPSGRRRAYNQLKKAGIEGLVAIGGDGTFK
                     GASLLGQEFGVPIVGVPGTIDNDLFGSDFTLGYDTAVNTAMTCIDNIKDTANSHNRLF
                     FVEVMGRDAGFIALRSGIASGAEAVLIPEAKTNIDDLIKILARNYKSKKSSSIVVVAE
                     GERTGGAFEIADMVKQKVPHYDMRVTVLGHLQRGGRPSCFDRVLASRLGMEAVKALRD
                     GKKDVMVGQIHTRIEYTPFEKACKHNQTISPVMLDMVRILS"
BASE COUNT          393 a          451 c          411 g          397 t
ORIGIN      
        1 tttaatttgt taataaatca attttaaact ttgacacact tttctgaata ctctcattaa
       61 aaattaagga attaaggccc tttttcactc ctcaaccctc actcctcaac cctcactcct
      121 cacccctcaa tcctctttcc ttttccctca aggtcttctc aaaaacatcc cggtcaaaat
      181 aatattcctt gtccccttcc tccagggatt tgggctcttc cagttcgata tcgtcctctt
      241 cctttatgct gaacctccgc ataaaatagg cgataaaaat gccgacaatg ccgccgaata
      301 aatggctttc ccaggaaatg ccttcttggt gtggaaggat gccgaacatc attccgctgt
      361 ataagatcgt gacgatgagc aggaggatga tcgatttgag gtcccggcgg aaaatgccgt
      421 tccagaagat aaaggcgacc agtccgtaga tgaccccgct cgcgccaata tgcaccgaag
      481 agccccgggc aaagagccag acggcgaggc cgcacaatac ccagatcagg gtaaaactca
      541 cccaggcgat tttccggtaa aaataaaaca acatcatgct gagcacggcc atgggggcac
      601 tgttggagat caaatgagcc caatccccat gcatcagggg ggcggtaatg atccctttca
      661 ggctctcggt atatcggggg atgatgcccc agcggtaggt attaaaaaaa gggaggttct
      721 ggataaaaaa accggtccag atggccgcaa ggatcagcag ggtgttccgg atgctgttgc
      781 ccgctcgtga tcgtttcatt attcaaaata atgagaaatt gaaacacccg gggcgggaga
      841 tcgacggatt agggtttgca gacggcgaag gtcaggaaag gatccggacc atgtccagca
      901 tgacggggct gatggtctgg ttgtgtttac aggctttttc aaaaggcgtg tattcgatcc
      961 gggtatggat ctggccgacc atgacgtcct tttttccatc ccttagggct ttgaccgctt
     1021 ccatgccgag ccgggaagcc aggacccggt caaaacagga aggcctcccg cctctttgca
     1081 aatggccgag tacggtaacc ctcatgtcgt aatgcggcac cttctgtttc accatatcgg
     1141 cgatttcaaa agcgcccccg gtccgctcgc cttcggccac cacaacgatg ctggatgact
     1201 ttttgctttt ataattcctg gccaggattt tgatcaggtc atcgatattg gttttggctt
     1261 ccgggatgag caccgcttcc gcgccggagg caatgccgct tcgcagggcg atgaacccgg
     1321 catccctccc catgacttcc acaaaaaaca gccggttgtg cgaattggcg gtatccttga
     1381 tattgtcgat gcaggtcatg gcggtattga ccgccgtatc gtaacccagg gtgaaatcgg
     1441 acccaaaaag gtcattgtca atggtgccgg gcactcctac aatgggcacg ccgaattcct
     1501 gtcccagcag ggaagcgcct ttaaaagtcc cgtccccccc gatagccacc aggccttcga
     1561 tgccggcttt tttgagctgg ttgtaggccc ttcttctccc ggaaggggtc cggaaggttt
     1621 cgctccttgc agactgcagg atggtgcccc cc
//
LOCUS       DAOQXT010000243         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_92340_length_1630_cov_3.047619, whole genome shotgun sequence.
ACCESSION   DAOQXT010000243 DAOQXT010000000
VERSION     DAOQXT010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92340_length_1630_cov_3.047619"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKM27_19780"
     CDS             <1..296
                     /locus_tag="PKM27_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR09568.1"
                     /translation="KGELQEKELVDLNALPKSSDEAEDWLVRAYAVGVKNLLQNKNEE
                     AIRVFKTILSTGFWNQLPFIAAEGELARLGGVEIKQPEVIELNTQDKKKPVKF"
     gene            406..1239
                     /locus_tag="PKM27_19785"
     CDS             406..1239
                     /locus_tag="PKM27_19785"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNR09569.1"
                     /translation="MKSENVLKPISGWGPLILAIVMPATFFLLRHPVYWATGGLLWLL
                     LLSGFIIVNPNFSKVLILFGKYVGTIREDGFFWANPFLVKKGISLRANNFDSERLKVN
                     DKRGNPIQISIILVWRVRDTFKAAFEVEDLYNFVKVQSDSAVRKLAGQYAYDHVDDHD
                     EVTLRSSSEEVNETLERELQERLEIAGIEVMEARIGYLAYAPEIAAAMLKRQQAEAIV
                     SARYKIVEGAVGMVENALHQLKERGIADFDDREKSRIVGNLMVVLCSDKETSPVIDMN
                     G"
BASE COUNT          428 a          378 c          430 g          394 t
ORIGIN      
        1 acaaaggcga acttcaggag aaggaactgg ttgacctaaa tgcactgccg aaatccagcg
       61 acgaagctga agactggctg gtacgggcct atgcagtggg cgtaaaaaac ctcctccaaa
      121 ataaaaacga ggaagccatc cgcgtattca agaccatcct gtccaccgga ttctggaacc
      181 agctgccctt tatcgctgcg gagggtgaat tggcccgact gggcggcgta gagatcaagc
      241 aacccgaggt tatcgagctc aacacccagg ataagaaaaa gccggtcaag ttctgatcgg
      301 tttatttttg agattggacg gatttaatga tcatttaatc gggaaaactg gttggtcggg
      361 aactttatgt tttgcaggat agttggttag gcaaaacgaa gttctatgaa aagtgaaaat
      421 gtactgaaac ccatttccgg ctggggtcct ttgatcctgg ccattgtaat gcccgctact
      481 tttttcctct tgcgtcatcc cgtatactgg gcaacaggag gattgctctg gcttttgtta
      541 ttgagtggat tcatcatcgt caatcccaat ttttccaaag tcctgatcct gtttggtaag
      601 tacgtcggga ccatccgcga ggacggattc ttttgggcca atcctttttt ggtcaaaaag
      661 ggcatctccc tgcgcgccaa caactttgac agcgaaaggc tcaaggtcaa cgacaaaaga
      721 ggaaatccca tccagatctc catcattctg gtctggaggg taagggatac cttcaaggcg
      781 gcctttgagg tcgaggatct gtacaatttt gtcaaggtcc agtccgattc agcggtccgc
      841 aagctggccg ggcaatatgc ctacgaccat gtggacgacc acgacgaggt gaccctgcgc
      901 tcatccagcg aagaggtgaa cgagaccctg gagcgcgagc tgcaggaaag actggagatc
      961 gccggcatcg aggtgatgga agcccggatc ggatacctgg cctatgcgcc tgaaatagcc
     1021 gcggccatgc tcaaaagaca gcaggccgag gccatcgtct ctgcacggta taagatcgtg
     1081 gaaggagcag tggggatggt ggaaaatgcc ttacatcagc tgaaggaaag gggcatagcc
     1141 gattttgacg accgggaaaa gtccaggatc gtgggcaacc tgatggtggt cctgtgttcc
     1201 gacaaggaga ccagcccggt gatcgatatg aacgggtagg cagccaaatc acgtttttac
     1261 cattttttca ggcgtattca tcatgaaaat tcctcatttg aaccatttct ttacaaaagg
     1321 cttttcactc accttatctt tgaatcgtaa atacgggact ggataacccg aatttttcat
     1381 caagattgag tttaagttaa ggataagaaa gggcttcgga cgaagccctt ttttttgccg
     1441 ttcccgggtg atcctgacct taggttgaaa gcaggtgcaa ttccctatat ttgcattcca
     1501 ccaaaatgca aaagaatggc ttacatcaac tggattgcgg ttattatttc actggccagc
     1561 agtctgatcc tcgcttacat ctggtacggg accggctgga tccataaaaa gggacaacag
     1621 gcggctcagg
//
LOCUS       DAOQXT010000244         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_1029
            NODE_101220_length_1542_cov_5.550101, whole genome shotgun
            sequence.
ACCESSION   DAOQXT010000244 DAOQXT010000000
VERSION     DAOQXT010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852372
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,920
            Genes (coding)                    :: 3,912
            CDSs (with protein)               :: 3,912
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101220_length_1542_cov_5.550101"
                     /isolate="AMR_MDS_1029"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1542
                     /locus_tag="PKM27_19790"
     CDS             <1..>1542
                     /locus_tag="PKM27_19790"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNR09570.1"
                     /translation="DRIGLNNYDVGHVFGTNAGGVALLGTACGANKGAGVSSTFGIYS
                     GTRFYHIPCHELGHQFSATHTFNFCDNENETTSSAYEPGSGSTLMSYAGASDCGTNYV
                     QGISDPYFHGYSLEQIKTYSRAGIGSLCGTLTDTPNDEPMAMAISPGNITIPVLTPFE
                     LEGTGIDDSETITYTWEEMDLGQKSPLGMPAGTAPLFRSVPPGNSGTRIFPRIGALLS
                     NTQESTEILPSTSRPLKFRLTVRDNDPQAGGVNWTELTLQSDVGSGPFRVTAFNTPDT
                     FSKGGMIYLTWDVKNTDRAPVACQWVDIWLSEDGGFSFNLPLKLKTANDGGEWIRLPD
                     HPVTNGRIKIKPVQHVFFDVNDADLVIRNDYPPRLEIGFIPDIQTLCAGDRGEYEVIS
                     SPSSSGDSVLIQISNPFQPFFTIAPSKSFIGPLDNIRLEVSVDPAAPPGQYLIPVLAT
                     GADTLSLNFTLRIVSANIAPIYPKDNEDQVPVLPIFRWEKLPAQDDYKIELSLDPGFR
                     SLVWS"
BASE COUNT          375 a          450 c          371 g          346 t
ORIGIN      
        1 atgaccggat cgggctgaac aattacgatg tcggccatgt attcgggacc aatgccggcg
       61 gggtggccct gctcggcact gcctgtggcg ccaacaaagg cgctggagtg agcagcacct
      121 tcggcatcta ttccgggacc cggttttatc acattccctg ccatgaactg ggccatcaat
      181 tcagcgccac ccataccttt aatttttgcg acaacgaaaa cgaaaccacc tccagcgctt
      241 atgaaccggg aagcggatcc accctcatga gttatgccgg agcgagcgat tgcggtacca
      301 attatgtaca gggcatctca gacccctatt ttcacggcta ttccctcgaa cagatcaaaa
      361 cctattcgcg tgcggggatt ggttccctct gtggaacgct tacggacacc ccaaatgatg
      421 aaccgatggc catggccata agcccgggca atatcacgat cccggtgctt acgcccttcg
      481 aactggaagg cacgggcata gacgactcag aaacgatcac ctatacctgg gaggaaatgg
      541 acctcggcca gaaaagtccc ctcggcatgc ctgccggtac agcgcccttg ttcagatcag
      601 tgcctcccgg caattccggc acccgcattt tccccaggat cggagccctc ctatccaaca
      661 cccaggaaag tacggaaatc ctgccttcca ccagccgacc actgaaattc aggctgaccg
      721 taagagacaa tgatccccag gccggagggg tgaattggac agaactgacc ttgcagagcg
      781 atgtgggttc aggcccattc agggtcaccg catttaatac cccggatact ttttcgaaag
      841 gaggaatgat ttatctcacc tgggacgtaa aaaacacaga ccgggcgccg gtagcctgcc
      901 aatgggtgga tatttggtta tccgaagatg gtggattcag tttcaatctg cccttaaaat
      961 taaaaacggc gaacgatggg ggcgaatgga tccggcttcc cgaccatccg gtcaccaatg
     1021 gaagaataaa aatcaagccc gttcagcatg ttttttttga tgtcaacgac gcggacctgg
     1081 tcattcgaaa cgattacccg ccaaggctgg aaatcggctt tattccagat atccaaaccc
     1141 tctgcgccgg ggacagaggg gaatacgaag tcatatcctc tccctcttca tccggtgatt
     1201 ccgtcctgat ccagatatcc aatccgtttc aacctttttt caccatcgcc ccttccaaat
     1261 cctttattgg accgctggac aacatcaggc tggaagtgtc tgtggatccc gccgcgccac
     1321 ctggccagta cctcatccct gtgttggcca ccggtgccga taccctttcc ctaaacttta
     1381 ccttgcggat cgtttccgca aatattgccc cgatctatcc aaaagacaac gaagatcagg
     1441 tgccggtcct gcctatcttt cgttgggaaa aactgcccgc acaggatgat tataagatcg
     1501 aattatccct tgatcccggt ttcagaagtc tggtatggtc cc
//
